LOCUS       DAOQJL010000001        23985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_928_length_23985_cov_5.073046, whole genome shotgun sequence.
ACCESSION   DAOQJL010000001 DAOQJL010000000
VERSION     DAOQJL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23985
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_23985_cov_5.073046"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLG78_00005"
     CDS             complement(<1..478)
                     /locus_tag="PLG78_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE21515.1"
                     /translation="MRPSVIASFLLIAAAFTCSRPPVDFREWMLSQMEFNRAATDHLL
                     DELEKTGKADEALAWRAGTNRAPLGWQLMHLAASEDRQATRLGAQSLISEKYAEDFKS
                     GKPAVSFIPKLPYVRQYLKDSRASLQKAIRDFDMSKMDQKVPETDNTFRKLFQIILW"
     gene            566..886
                     /locus_tag="PLG78_00010"
     CDS             566..886
                     /locus_tag="PLG78_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21516.1"
                     /translation="MTKQTTFTLLLLVMLSTPALAGPEDQWYRSTFPNGAASLKYHCK
                     KHGNGKPCDQYTRDAISFCKSNLKSARPMTLKDGTPAIKIRRGTVGGYFKENCSQIVT
                     FWYN"
     gene            905..1231
                     /locus_tag="PLG78_00015"
     CDS             905..1231
                     /locus_tag="PLG78_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21517.1"
                     /translation="MNQKLHHLKESFALLKSMPFPSSIDDALLGDLHAELAEYDGYVV
                     GILETVLSSGQPGKEEVDSAPQLTKALESTQDARVVPLRDYLKQIKACLKAAEEAGLI
                     HFSRGK"
     gene            complement(1275..2159)
                     /locus_tag="PLG78_00020"
     CDS             complement(1275..2159)
                     /locus_tag="PLG78_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21518.1"
                     /translation="MLLLIDDNLTICEVVKKLFSGVYDLRITHTAAEGWAALEANAAS
                     VKAILLDWELPDQNGIDFLRKLKQDSRFRLTPVLMLTGRTDEKDIEAGIAAGALYYVT
                     KPFDHRILSALVETALRDFSSAHKTSTVRLSDYESLLTELSFRFSTVSQAGALSQLLA
                     EYCPSPDAARLGLHELLVNAIEHGNLEISHAEKSKLLGEGTLPDEMARRQNVAPYANR
                     SASVRMIRTSEEVRFIIRDQGPGFNYKAFLNLSADRAFAQHGRGIALARSLSFDHLSY
                     VPPGNEVHGRITVPRSAS"
     gene            complement(2163..2981)
                     /gene="pyrF"
                     /locus_tag="PLG78_00025"
     CDS             complement(2163..2981)
                     /gene="pyrF"
                     /locus_tag="PLG78_00025"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNE21519.1"
                     /translation="MSTFYDRFLARRGALRSVLCVGIDPDPAAIPNSDSGIEGTFSFL
                     REIIQATAPYACAYKPNVAFFEVLGPAGWDLFGRIVQCIRREAPEALIVADAKRGDLA
                     NTARFYAETFFKTYNCDGLTVNPYMGMESLAPYDQFSDRGVFALCLTSNPGAIDFQMQ
                     GSPPLFEQVARSVQERNAITRNYWMVAGATRDADSLSRIKSIAPSVPLLIPGVGAQGG
                     ELAVVLRNCGADVLVNASRSILYAYKEHSGVSRGEAAARSAKNVVDEMRSLLSF"
     gene            3072..4955
                     /locus_tag="PLG78_00030"
     CDS             3072..4955
                     /locus_tag="PLG78_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE21520.1"
                     /translation="MYREAAQLFGNLPAFGTRQKDKTWKQISYAELYETGVNLATALI
                     DLGVQQREHVALLADNRVEWIIADYGCQLAGAADVPRGTDVTDADIQYILPHSDAKVC
                     FVEHKEMLSKVQKNLDKCPNIKTLIVMDQGATASGGNVLSMYDLIAKGKELRAKGDKR
                     VEERIESTKPDDLFTLIYTSGTTGAPKGVMLMHSNMISQTQNIPIPITTADRILSILP
                     VWHIFERVFEMMAVSQGACTYYTNIRNIGDDMKNVKPTFMGSAPRLWENIYLKILNNV
                     KGAPPIRRALFNAAYFCSLMFQGSLFFLTGRSLDMEGRNPLVSIAMGILHILRLTLFA
                     IPYFLLDVIVLKKLRGATGGALKGSVSGGGALPLHVDQFFNYIGIPVLEGYGMTETCP
                     VLAVRSWKKLVIGSVGPIFPGTQIRLMDLNTGDVIYPNDKRRAMGRGIKGEIHAKGPQ
                     VMKGYYKNPEATAKVLKDGWMNTGDMGMITFNDCVKIMGRSKDTVVLQNGENVEPVPI
                     ENKMCESVFIDQVMVVGQDQKFLAALVVPSVEEFKKFGIPAETVEELARNPEVLRVIK
                     EEIARLVSGENGFKAFERMQDARVVPKAFEVGDEMTNLFKLKRHIITDKYKSLIDDIY
                     KEK"
     gene            4984..5322
                     /locus_tag="PLG78_00035"
     CDS             4984..5322
                     /locus_tag="PLG78_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE21521.1"
                     /translation="MMTKAEFSGNTLHLSFHVERLDMSMVRALRDEVRGHLVGRPTPV
                     FVDMSNVTYMDSSGMGTLKLIMDDVTAYGGQLVLRKINQPQMMLLKLSKLDSYFQFEE
                     IKKQPGLHDM"
     gene            5373..6698
                     /locus_tag="PLG78_00040"
     CDS             5373..6698
                     /locus_tag="PLG78_00040"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21522.1"
                     /translation="MRRRNKVPASEKYRPANVARIVYNLVFFAVFVYFKLTVVYPLSV
                     LHLVFAVSWITLIELEILIEKEHWWSGYIPATADLLWISVMVYVTGNISSFVIIGYFI
                     SISLSSMTDSRNYGIYNVILSSVLYAGVGASVYAGLLPAVNILMDPVRPPLLPLVIAS
                     VSMLGSFYIVNQVVSRLFLSLNGEVRTRKKAEDRLRRDLELAHNVQQSIIPAGNRFPV
                     RSEFRFGSCYIALDSVGGDLYDVIPLTGNRFGFLIADVSGHGVSAALVTTMAKASFSN
                     AAPANTLAHEVCATVNRDMFKFIGDSVNYLTAFYCVVDLNTGSVNYCNAGHHPALLVR
                     GSTCQELKSENTFPIGVWAEFDFESGWVSLAPGDRILLFTDGITEASNPAFAQYGESR
                     FQDFIAQSASLNASDFVENLILDLKTFCAGTPIHDDRAVLCIDYLRDSD"
     gene            6730..6945
                     /locus_tag="PLG78_00045"
     CDS             6730..6945
                     /locus_tag="PLG78_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21523.1"
                     /translation="MEEKFENIRKNTTCSECGGKEMAFGYLFEGTGAVKKKGSLLGLD
                     AFSGVGNMIMIVCQGCGFVAKSFVIAR"
     gene            complement(6972..7643)
                     /locus_tag="PLG78_00050"
     CDS             complement(6972..7643)
                     /locus_tag="PLG78_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21524.1"
                     /translation="MEHSDTQSIAKIDWIVHSLDEEKTHASLWYYGFTGLYATSTVAS
                     FTIASTSHDPIVRATQRVSGWQSLIATGGMLLGPVPSAWAADDIRSMPAGTEAEIKSK
                     LDAAESALRTSAETQSFNRSWINHALGVAVAGGGAAVIRYTYGHKIDKAGGGGRSVRE
                     ATVNFWSSLIVGELQIWTMPVLSMDRWDEYSKKKASESLSIESVRLVLVPANDGLRAG
                     IAMRF"
     gene            7808..8998
                     /locus_tag="PLG78_00055"
     CDS             7808..8998
                     /locus_tag="PLG78_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE21525.1"
                     /translation="MEQRGNRKWLVLAAFAGTALVSQMLWLNFATIAITIQNRYTVDE
                     STANLLTLVFPLLYLVFSIPSGILIDRKGYRVSVLWGSFMQAAASCIRIYDESFYALL
                     AGQVGIAVAQPFIVNGISKLAADWFDEDEGAAANGIGTVGMFAGMALALGLTPVFVEH
                     WGLRTAMIIFSLVSVAAAAIYALIDRKSISASKESTSSISFGPVLKDKRLQLLFFLAF
                     LGLGFFNGLTAWLEQILAPRGISAIDAGIIGSALIVGGIVGAAIVPNFSDKFKKRKPF
                     LLSCVIAAVCLTYPLGMGSRFSILVTLAAALGFFFLPAFALLLEMCSEVAGEKHTGAA
                     TGILMLTGNAGGVVVMLAMDAIRFGSPSYDNAVYLLVVLLAIALFASVFLPETYGAKP
                     IEDK"
     gene            9059..9721
                     /locus_tag="PLG78_00060"
     CDS             9059..9721
                     /locus_tag="PLG78_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNE21526.1"
                     /translation="MMPNTTLSRAYYALAIACILDVIAIAAGASIPDFYGRPVLLLSF
                     AVVVFKNAAHNSHRLISLGMVAAALVEAMQMLGIGFAWIASATAVYYLAYSVAFALRG
                     GKIKSKLEATGFAVYALYSIVMFLWLEPYGFLRMASILYTAVVILFGGLSSRPVFARK
                     EGAAWSRLLAGAVALLVISDSMRAISIFKEPVSEALILAPYWAGQFGLALWPIFDKKK
                     QL"
     gene            9796..11880
                     /locus_tag="PLG78_00065"
     CDS             9796..11880
                     /locus_tag="PLG78_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline ceramidase"
                     /protein_id="HNE21527.1"
                     /translation="MNKFKTSYTIGLLLFAIYCGADKEKGPMIAPIPGASASNHNDLT
                     TAGAFTGTCFTADKFNVGRGIYDITGSAAELGMMGYAMVDQKTAGIHMRLRARAFLIE
                     SPCNGKRLVYVSADLGQVFQSVKQGVVKKLVAAYGDMYSDENVILSATHTHSGPGGYS
                     HYALYNLTILGYDQQNYDAIVNGIFQAIVRAHNNAKPATIELATGDVNDASMNRSPIA
                     YAANPAAERAKYSADIDKKMTLLKFRGTDGKELGLLNWFAVHATSIGNKNHLISGDNK
                     GYAAYRFEKAKGTNYASADTFVAGFANANEGDVSPNLWGVPDLVHDFDRTYTIGNRQY
                     ETANTLYNQGGKVLSGGVDYRHAYVNYSQVTVDAQWTGGAAKSTCKAAIGVSFAAGST
                     EDGPSGVPLITEGMTYGGASWPMFTLVPDDQACHEKKIILLPTGRMTPYPWTPEVLPV
                     HIARIGSLAIVAVPFECTTMCGRRLRDTVGAELKADGVTDVVIAGLADAYAGYVATKE
                     EYSVQHYEGASTHFGPNQLGAMQQEYAKLARAMRNGETVNPGPTPRDLSNKQSSLITG
                     VVFDDVPLFKSFGDVYQDAAASYSRGTVASAIFWGAHPRNNLRTQGTYLEVQRWNGSS
                     WVTVASDWDPETKYEWARDGIANSKVTVKWSIPATAATGTYRLRQYGNWKSGWTGAIS
                     AYTGTSRSFTVN"
     gene            11930..13012
                     /locus_tag="PLG78_00070"
     CDS             11930..13012
                     /locus_tag="PLG78_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNE21528.1"
                     /translation="MDSGKLVSVLTIDDSATVIAYLRTLLKERQIMTDGCGSLAEARS
                     ILAQKKFDLIFLDLVLPDGSGIDLLKEIRAKDKTTPILMLTGRGDVKTVVSAMALGAD
                     GFVEKQDLFLDDFMLAVDRAVEHRHGILAREQLEQLKRDMYAMITHDLRNPIGNAGAA
                     ADLLLTMTPDDPDHKQVLSIIRTSIDSALGLMEQYLDYEKINAGFLELHKSKCNLADL
                     VTASVNNVGMVARKRKQELTVDAQAFEAAVDPLRMQQVMENLISNAIKYTQEGGKISV
                     RLYGTNGSIIFEVEDNGFGIPPAELETIFGKFKQLTNGTKLSRGFGLGLMIAHEIVEA
                     HGGTIKAESGGSIAKGSRFTVRLPAV"
     gene            complement(13057..14091)
                     /locus_tag="PLG78_00075"
     CDS             complement(13057..14091)
                     /locus_tag="PLG78_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE21529.1"
                     /translation="MKVLVTGGCGFLGSHVCELFRKEGWEVVSYDSMTKYELAKTGYG
                     TEKTRNFNWDYLKALGCEMVKADVCDEQAMKDHSSGCNFIVHTAAQPAMTISWEDPRL
                     DFNTNVLGTFNVLDAARKHRIPVVNTSSIHVYGNSINDTLKEGATSYERTPVAIDEKH
                     PVMVGQLSPLHASKMAAEHYVRTFTDMLQTPGASFRFTGIYGPRQFGGEDHGWVANFA
                     IRAVFGLPLRVFGTGKQSRDILYAGDGAQSYLAYFKNPIPGVYNIGGGPEHKISLIEC
                     IAMIGEILGTKPVIEFHPERPGDMRYFICDITEARKFGFNPKVKPREGVEKLIRWVEE
                     NKSVFNITGK"
     gene            complement(14120..15277)
                     /locus_tag="PLG78_00080"
     CDS             complement(14120..15277)
                     /locus_tag="PLG78_00080"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNE21530.1"
                     /translation="MSKIISQAKAGQGRLFVQFGGQGAPWYKELAKYYKEPSMKKFFD
                     VVLRAIEEERPHVDGTVGLPHGLDARAWLEDESKIPSEDYLACAAVSIPMIQVTQLAH
                     FENTVNKGISRKDLVAATEATSGHSQGLIPASLVALDLDGDAYYAAAAKYVKYLLYLG
                     VRAQEVYPFFAPTADEIARSEKAGNKAPAPMMAVVGDSHETIQAMVDATNKELPADQK
                     IYVSLYNSPSNRILSSYRASLVRFTEMHGAALAEKKVKAVYLRTSCPFHCPLMDPIRP
                     LFEADIKRIGFSYPGSALKVPVYSFYDGRNLQGDADIAIGMYLDMAINPLYWDKSMKP
                     VADSSKITTIVDFGPGKTSQRLSEDTLQGLGVEKNVLACAVPKDLEALLAP"
     gene            complement(15288..15851)
                     /gene="efp"
                     /locus_tag="PLG78_00085"
     CDS             complement(15288..15851)
                     /gene="efp"
                     /locus_tag="PLG78_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE21531.1"
                     /translation="MMDPIDLRKGQVIKVDNDLYTVTFSQFVNPGKGSAFVRTKLRNL
                     KRGTVVERTFKAVEKIDDVDLEKRYMTFLYKDGDHLVFMDKDDYEQLSIPMVLVEDIV
                     PFMKEEMPFEITFYEGNPVGVTPPNFAELEVTYAEDAIKGDTVGTSRKKVTVETGGEV
                     LVPLYVKQGDRIKIDLREISFVERVSK"
     gene            15850..17001
                     /locus_tag="PLG78_00090"
     CDS             15850..17001
                     /locus_tag="PLG78_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNE21532.1"
                     /translation="MRIEIYKKGRAVKQNYDWKEELRSRISSADELLEFMPDLPESPL
                     FRRLYASVEDFRKVAEAMAGEFRFSVTPYYLSLANLENARCPILLQILPRREELEDQV
                     FQFPDPLAEDSHHPVDGLTHRYPDRVLWYLSHNCAVYCRFCMRKRKVSRSNSAPDRSS
                     YSAALDYIRNHKEIKEVILSGGDPLSHSDQTIQFLLTELRTISHLSSLRIHSRMPVTL
                     PSRITDQLTDVMRQAFPVTLVTHFNHPVEITEEATSAVRRLRMNGVTVLNQTVLMRDI
                     NDSTQTIEELMLGLLRIGIKPYYLHQCDEVHGVSHFRANIETGLQILRDLQGRNPGIA
                     IPRYVIDLPGGGGKVPLENQYLRRRTDDGLEFENWSGLKFTTVQDREDK"
     gene            17042..17719
                     /locus_tag="PLG78_00095"
     CDS             17042..17719
                     /locus_tag="PLG78_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE21533.1"
                     /translation="MKQSELSQKLQIKSGMRIGTMNAPDMFARKLAPVPEGSELSPVA
                     PYDHLTVFVNRKAELDRFALGAMKSMRPGGILWFAYLKKASGAKSDVTRDDGWDALTR
                     AGWGPVSLIAIDGTWSALRFRPLTKEEQEDRANRKVPLDEGIEKKPSKPKPSVARKPP
                     AVPADLTAALAKNKKAKQFFESLAPSHAKEYIVWITEAKKAATRETRIAKTIEKLLQK
                     KKNPTIK"
     gene            17716..18408
                     /locus_tag="PLG78_00100"
     CDS             17716..18408
                     /locus_tag="PLG78_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNE21534.1"
                     /translation="MNLHCILLSGGTGERMAAGKPKQFIELLGIPVLRWSALTLSNFS
                     KRRLTVVAPANLLAETRSILEGIDSVVVPGGSTRHDSTLCGIRSIAKEIHLQSDIILI
                     HDAARPVIRHAELSRLCDLFKDETILCSSLVGPIVETIVQSRDGKTSDGIADRDQLRA
                     VKTPQALRANALRALLEVEGNFTDLLSWAHATGLKAGLALAEETNVKLTSQGDLDLML
                     SLLNRLKIDGPS"
     gene            complement(18377..18871)
                     /locus_tag="PLG78_00105"
     CDS             complement(18377..18871)
                     /locus_tag="PLG78_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE21535.1"
                     /translation="MPTHYKGSDAEVRALDAFIKLARASSSLASRLEGSMHLEGLTEP
                     QLGVMETIYHLGPMCQKELGAKQLSTGGNITLIVDNLEKRGLVERVRNTEDRRYITVH
                     LTEEGKKLMKRVFPRHAKAIAEEFAVLSPDEQTMLSSLCKKLGLGKPEYEEALTKVRR
                     SSDG"
     gene            19003..19638
                     /locus_tag="PLG78_00110"
     CDS             19003..19638
                     /locus_tag="PLG78_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE21536.1"
                     /translation="MNILVIGASKGTGAICVQNALERGHSVTAFSRSPEKLALNHSRL
                     RLYPGSFHDAANVQAAVPGHDAVIITASASSMQAFKENPNYFSNGTAIVIDAMKKHRV
                     PRLVVLSALGVGESGKLMPWPLRKIVVSWLLKLPFADHERQETMVQSSGLDWVIARPG
                     RLTDGPAKKQYQKKSQIESIPTSISRADVADFLVEACEPGEWVRKAVQIGG"
     gene            complement(19639..20343)
                     /locus_tag="PLG78_00115"
     CDS             complement(19639..20343)
                     /locus_tag="PLG78_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE21537.1"
                     /translation="MWFDYSAHPGKKPWASNILNFIGVLFGILFFKVKIEIDPDAQKN
                     GPVLILSKHSAEFDIPLGYHAMKRAFGRHAWCIMKASLVRPRYLGFFWKIGGIPLERE
                     DPQQSKNYLVYARKVLYEKDITGLKDGDGNLMVLFPEQTTYWRQMGEGKLAGFRFLAG
                     KPARPLACNSVGFRYKPGFPRTSVVIRFGPTRYFTADMDHGLFLHERMKEIAELSALE
                     YSFPAPRSRKAEAVVG"
     gene            20434..21234
                     /locus_tag="PLG78_00120"
     CDS             20434..21234
                     /locus_tag="PLG78_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNE21538.1"
                     /translation="MKIPMYQVDAFTQKVFAGNPAAVCPLETWLPESTMQAIAAENNL
                     AETAFFASAANDFQIRWFTPEAEVALCGHATLASAVVARHLNLFQDRICFHSKSGPLF
                     VDRKDDLYTLNFPLRTIGPEIQVSPAIAECLGTPPVRLFRSEPNYLAVFSSEEEVRRL
                     NPDFRKMKEIAGHGIIATARGTEFDFVSRFFAPAIGVDEDPVTGSAHCTLIPYWSAVL
                     NKTRMVAYQASRRGGVLNCELKDERALIGGYGALYSEGFIFISEAGTR"
     gene            21507..22307
                     /locus_tag="PLG78_00125"
     CDS             21507..22307
                     /locus_tag="PLG78_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21539.1"
                     /translation="MKFESSANRNEKMHKPFDLRLDLNAKTMSFDGKTIPLEKFNFQS
                     ASYHTMGDYGFESEMLFVSGKGKNREFLIKTRSAPEHEDEFLYFTSSRNEIHPIQATH
                     WRYVLDPKAGGETHFAQLHADVAIPGQGPAQIKLDVVRTPATDVGFWLLRKNKSPTRF
                     WQTNDSNTRRFRFCANSSASDEAGCSVLTIANGTGNPACSGVEIGTIVVMLNQGKAQI
                     LSDDQKGHLLDEDCKVVYSLEYREEINAVSIKGSEGPAKVFVEKQPDP"
     gene            22340..23182
                     /locus_tag="PLG78_00130"
     CDS             22340..23182
                     /locus_tag="PLG78_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE21540.1"
                     /translation="MKWIKTTFLLLALCGPLLAEPTVVRGSRLLDIKKSGKIKIGIQN
                     EYRPFRIENPKPGYPGIDVELANALGEALEVEVEISYLPLDQLLIETNEGKLDVSLGG
                     ISSTLERARKVNFSDPYMKTTPAGLLARDSLPPESESVNYARRQFKSLSDLKYLGPIT
                     IGVRAGTANETLLRTDPDFQKHKIKVFQDRTIALAALESREIDLLVADDVYLRALLLK
                     RPELMNRFLPLLDKYLEDHISMAIPPGDPELWNYLNFFIKEMIRTGRMQTIINKYFQS
                     GAWF"
     gene            23186..>23985
                     /locus_tag="PLG78_00135"
     CDS             23186..>23985
                     /locus_tag="PLG78_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21541.1"
                     /translation="MRRISPSRLTGLALVIASSLILAFSSRPLHAQESSTPIADRFSY
                     RGDMFIRGIYLARDLPLKSQSYNTTSRNREEQDFYSARLRLDLAFRASQYVDVLYGLE
                     VGELTFGRDNASRAGPGSGGKGAGAANLETRELILKFRNYPESLSFSAGILNFDTPRG
                     LVTGTSGAGAKFSAEMRALHSSVDAYYIRSEDNSTLDGDSNGFSDSNFRNVNMGLISW
                     KFSGIPGMRSEFYGMYRQDDDPSLTAGDSGETSRVYWGGIFTQYKAGQ"
BASE COUNT         6189 a         6083 c         5883 g         5830 t
ORIGIN      
        1 gccacaggat aatttgaaat agtttacgaa atgtattgtc tgtttccgga actttctgat
       61 ccattttgct catatcaaag tcacgaattg ctttttggag agacgcacgc gaatctttca
      121 aatactggcg cacgtagggc agcttcggaa tgaacgacac ggctggtttg ccgctcttaa
      181 aatcctccgc atacttttca gatataagag actgggcccc caggcgagtg gcctgtctat
      241 cctcggacgc ggccaggtgc atcaattgcc acccgagagg tgcccggttg gtccctgcac
      301 gccaggcgag agcttcatct gctttgccag tcttttcaag ttcatcgaga agatgatccg
      361 tggccgctcg attgaattcc atctgcgaaa gcatccactc tctaaaatcg acgggtggtc
      421 tggagcaagt gaatgccgcg gcgatcaaaa ggaaagaagc gatgacagat gggcgcatgg
      481 aaaacacgaa aacaatgagc tggcttcagg ctatgaattt tctgattgca cccaatcgtt
      541 ttagagttct ttttgcccgg gtatcgtgac aaagcaaaca accttcactc ttctacttct
      601 ggtgatgcta agcactccag cgctcgcagg gccggaggac caatggtaca gatccacttt
      661 tccgaatggt gctgccagtc tcaaatatca ctgcaaaaaa cacggaaacg gaaagccgtg
      721 tgatcagtat acgcgcgacg caatttcatt ctgcaaatcc aatttgaaaa gtgcgcggcc
      781 catgactctc aaagacggaa ctccggccat caagattcgc cgcggaacgg taggcggata
      841 cttcaaagaa aactgcagcc agattgttac cttctggtac aattagttta gatcaactgt
      901 cgccatgaat caaaagttac atcatctcaa agaatcattt gctctcctca agtcgatgcc
      961 atttccatcc agcatcgatg atgcattgct cggagatctt catgcggaac tggcggagta
     1021 tgacggctat gtcgtgggca tcctggaaac cgtcctttcc tcagggcaac ccgggaaaga
     1081 agaagtggac tctgctcctc agcttaccaa agcattggaa tccacacagg atgcgcgagt
     1141 cgtcccgctt cgcgactatc taaaacaaat caaggcctgc ctgaaagcag cggaggaagc
     1201 aggcctgatc cacttttcgc gggggaaata gaaaactgag ccgctgctcg gagacatcaa
     1261 cgcccggcaa gacttcacga agcacttcgc ggaaccgtta tgcgaccatg aacctcgttt
     1321 ccgggaggca cataagaaag atgatcaaac gacaaagaac gagccagggc aatgccgcga
     1381 ccgtgctgtg caaaagctct gtccgctgac aaattgagga atgctttata attaaagccc
     1441 ggtccctgat ctcgaatgat gaagcgtact tcttcgcttg tgcgaatcat ccgaacgctg
     1501 gcagacctgt ttgcataggg tgcgacgttc tgcctcctcg ccatttcgtc aggcaaagtt
     1561 ccttctccca gcaacttaga tttttcagcg tgggagattt ctagattccc gtgctcaatc
     1621 gcattcacga gtaactcatg tagaccgagc ctcgctgcat cgggtgaagg acaatactca
     1681 gctaagagct gagacaacgc gcccgcctgg ctcactgtgc tgaatcgaaa cgacagttcc
     1741 gttagaagcg actcatagtc tgaaagccga acggtgctgg ttttatgagc agaactaaaa
     1801 tctcggagcg cggtttccac cagcgcgctg agaatgcgat gatcaaacgg ctttgtaaca
     1861 taataaagcg ccccggccgc aatccctgct tctatgtcct tttcgtccgt gcggccggtc
     1921 agcatcagaa caggggtcag cctgaaacga gaatcctgtt ttaattttct taaaaaatct
     1981 ataccatttt gatccggaag ttcccagtcc aggagaatcg ccttgacgct tgctgcgttt
     2041 gcttccaggg ctgcccagcc ttccgcagcc gtgtgcgtaa tgcgcagatc atatacgccc
     2101 gagaagagct ttttgacgac ttcgcaaatc gtcagattgt catcgatgag cagaagcaca
     2161 gcttaaaagg aaagaagcga tctcatttcg tcgaccacgt ttttggccga acgagcagcc
     2221 gcctcacctc tcgagacacc tgaatgttcc ttgtatgcgt agagaatgga ccggctcgca
     2281 ttcacaagaa catctgcgcc gcaattgcga aggaccactg cgagctcacc gccctgtgca
     2341 ccaactccag gaatcagaag tggaacggac ggagcaatcg atttgatacg agaaagagaa
     2401 tccgcatctc tcgtcgctcc tgccaccatc cagtagttgc gagtgattgc gtttctctcc
     2461 tgtacagatc gcgcgacttg ttcaaaaagt ggaggtgacc cctgcatttg aaagtcaatt
     2521 gcaccaggat ttgatgtgag acataatgcg aagactccgc gatctgaaaa ctgatcgtat
     2581 ggtgcaagcg actccattcc catgtacgga ttgaccgtga gcccgtcgca attgtaagtc
     2641 ttgaaaaatg tctccgcgta gaagcgtgcc gtatttgcga ggtctcctct cttcgcatcc
     2701 gccacaatca gcgcctccgg cgcctctctt cgaatgcact gcacaattct accgaacaaa
     2761 tcccagccag ccggacccag aacctcaaag aatgcaacgt tcggcttgta tgcacacgca
     2821 tagggtgcag tagcttgaat gatctctctc agaaaagaaa acgttccctc aatacctgaa
     2881 tcagaatttg gaatagctgc gggatccgga tcgattccaa cacaaagaac cgagcgcaat
     2941 gcacccctgc gggctaaaaa acgatcataa aaggttgaca tccagacttc cgtacaattc
     3001 tttttctgag cctacttttc ggggggagga ctcacgtgcc aacaaggatt actgcgaaaa
     3061 cactgcctgc gatgtatcgc gaggcggctc agttgttcgg aaatcttcca gctttcggga
     3121 caagacaaaa agacaagaca tggaaacaaa tcagctacgc agagttatat gaaactggcg
     3181 taaatcttgc gacagcgctc attgatctgg gagttcagca gcgcgaacac gttgccctcc
     3241 tcgcggacaa ccgcgtggaa tggatcatcg ccgattacgg ttgccagctg gccggagcag
     3301 ctgacgttcc tcgcggaaca gacgtaacag acgctgatat tcagtatatt cttccgcatt
     3361 cagatgcgaa agtgtgcttt gtagaacata aggagatgtt gtccaaagtt cagaagaatc
     3421 tagataaatg cccaaacatc aaaacactca ttgtcatgga ccagggagca actgcgtccg
     3481 gcggcaacgt tttgagtatg tatgacctca tcgcaaaagg aaaagagctt cgcgcgaaag
     3541 gagacaagcg agtagaagag cggattgaaa gtacaaagcc agacgatctg ttcacgctga
     3601 tctacacatc tggaactacg ggtgcgccaa agggcgtaat gctcatgcac tcgaacatga
     3661 tttcgcagac tcagaacatc ccgatcccaa ttacaactgc ggacaggatt ctatccattc
     3721 tcccagtatg gcatatcttc gagcgtgttt tcgaaatgat ggcggtaagt cagggtgcct
     3781 gtacctacta tacaaacatc cggaacattg gcgatgacat gaagaacgtt aaaccaacgt
     3841 tcatgggatc tgccccgcgt ctctgggaaa acatctacct gaaaatcttg aacaacgtaa
     3901 agggtgcacc tccaattcgt cgcgcgctct tcaatgcggc atacttctgt tcattgatgt
     3961 tccagggctc cttgttcttc ctcacaggaa gatctcttga tatggaagga agaaacccgc
     4021 tggtttccat cgcaatgggg attctgcaca ttcttcgcct tacgctcttt gcaattccgt
     4081 acttcctcct cgacgtcatc gtcttgaaga agcttcgcgg agcaactggc ggcgcgctca
     4141 agggttctgt atccggtggt ggagcacttc ctcttcatgt ggatcagttc ttcaactaca
     4201 ttggaattcc agtcctcgaa ggctatggca tgacagagac ttgtcctgtt ctcgccgttc
     4261 gttcgtggaa gaaactggtg atcggtagcg ttggacctat tttccctggc acccagattc
     4321 gcttgatgga tctgaacact ggagatgtga tttatccaaa cgacaagcgt agagctatgg
     4381 gccgcggaat caaaggcgaa attcacgcca aaggcccgca agtaatgaaa ggctactaca
     4441 agaacccgga agctaccgcc aaggtactca aagacggctg gatgaacacc ggtgacatgg
     4501 gaatgatcac cttcaacgat tgcgtgaaga tcatgggtcg ctcgaaggac actgtggttc
     4561 tgcaaaacgg tgaaaacgtg gagccagtcc caattgaaaa caaaatgtgc gaatctgtgt
     4621 tcatcgacca ggtgatggtt gtaggtcagg atcagaagtt cctggccgcg ctcgtcgttc
     4681 cgtcggtaga agaatttaag aaattcggaa tccctgcgga aacagtagaa gaacttgcga
     4741 ggaatccaga agtccttcgc gtaatcaaag aagaaatcgc aagactggtc tcaggtgaaa
     4801 acggtttcaa agcatttgaa cgcatgcagg acgcacgtgt ggtcccaaag gctttcgaag
     4861 tcggtgatga aatgaccaac ctcttcaaac tgaagagaca catcatcacg gacaaataca
     4921 agtcgctcat cgacgacatt tacaaggaaa agtgagaaga atgaagacgg agaaccaggc
     4981 ccaatgatga caaaggctga attctccggc aacactctcc acctatcctt tcacgtggaa
     5041 cgcctggaca tgagcatggt ccgggcgttg cgcgatgagg tgcgcggcca cctcgtggga
     5101 agaccgactc ccgtatttgt agacatgtcc aatgttacgt acatggactc ttccggtatg
     5161 ggcacgctca agctcatcat ggatgacgtt acagcgtacg gcggccagct agtgttgcga
     5221 aagatcaacc agcctcaaat gatgctgttg aaactttcca aactggattc gtacttccaa
     5281 tttgaagaaa tcaagaagca gcctggcctt cacgacatgt aatcgccgtt agggcttgac
     5341 agcttcttcc gtttccatca cactccagca tcgtgcggcg gcggaacaaa gtaccagcgt
     5401 ccgagaaata ccgcccggcc aatgtagctc gcatcgtcta taacctggta ttcttcgcag
     5461 tctttgtcta cttcaagctt acagtcgttt acccactttc cgttttacat ctggtcttcg
     5521 cagtttcctg gatcacgctg attgaactag aaattctgat cgaaaaagaa cactggtgga
     5581 gcggctatat tccggccact gcggacctac tctggatcag cgtgatggtg tacgtgaccg
     5641 gaaatatttc ctcatttgta atcattggct attttatttc catttcgctc tccagcatga
     5701 ccgattcgcg caattatggc atctacaatg taatactgag cagcgttttg tacgccgggg
     5761 ttggcgcgtc tgtatacgct ggccttctac ctgcagtcaa cattttgatg gatcctgttc
     5821 gaccgccgct cttgccgctt gtgattgctt ccgtctccat gctgggctct ttctatatcg
     5881 taaaccaggt agtatcgcga ctttttcttt cgctcaatgg agaggtccgc acgcgaaaga
     5941 aagcagaaga cagactcagg cgagacctcg aattggcgca taacgttcag caaagcatca
     6001 taccagcagg aaatcgcttt ccggttcgat ccgaattcag attcggatcc tgctacattg
     6061 cactcgatag tgttggtgga gatctgtatg atgtgattcc tctcaccggg aatcgatttg
     6121 gatttctcat agcagatgtt tctggtcacg gtgtttcggc ggcgctcgtc accaccatgg
     6181 ccaaagcatc attcagcaac gcggctcctg caaatacact ggcacacgag gtttgcgcga
     6241 cagtcaacag ggacatgttc aaattcattg gagacagcgt aaactacctg actgcgttct
     6301 attgtgttgt agatctcaac actggcagcg tcaactactg caatgccgga catcatccag
     6361 cactgcttgt tcgaggttcc acctgccagg aactgaagtc tgaaaacacg tttccgatcg
     6421 gagtgtgggc agaattcgat ttcgaatcag gctgggtttc tcttgcgcct ggagatcgca
     6481 tcttgctctt cacagatggc attactgagg catccaatcc tgctttcgct caatacggag
     6541 aatccaggtt tcaagatttt attgcccaat ccgcttcatt gaatgcttct gattttgttg
     6601 agaatctgat cctcgattta aaaacttttt gcgcgggcac gcccattcac gatgatcgtg
     6661 ccgttctgtg catcgactat cttcgcgatt cagattgacg cacaataaac atatgccgtc
     6721 actcatgata tggaagagaa attcgaaaac ataagaaaga atacgacgtg ctctgaatgt
     6781 ggcggaaagg aaatggcctt tggctatctg tttgaaggaa ccggagcagt gaagaagaaa
     6841 ggatcgctac tcgggctcga tgcgttttca ggcgttggca acatgatcat gattgtgtgc
     6901 cagggatgcg gatttgttgc aaagagcttt gtgatcgccc gctgagtttt caaagaatac
     6961 ttacgcaatc tctagaagcg catcgcaatt ccagcgcgaa gaccatcgtt tgcgggaaca
     7021 agaaccagtc gcacactctc tatggaaaga gactcggagg cttttttctt cgaatactca
     7081 tcccatctat ccatggagag aaccggcatg gtccagatct gcagctcacc tacaatcaga
     7141 ctgctccaga aattcacggt ggcttccctt acagatcttc caccgcctcc tgctttgtcg
     7201 attttgtgcc cataggtata acgaatcaca gcggcgccac ctcccgctac agccactcca
     7261 agagcgtgat tgatccaact ccgattgaat gattgagtct ccgcactcgt tcgaagcgcg
     7321 ctttctgccg cgtcgagctt gcttttgatc tccgcctcag ttcctgcggg catactgcga
     7381 atgtcatctg ctgcccacgc agaaggaaca ggaccaagca gcatacctcc cgtcgcaatc
     7441 aaagactgcc acccgctgac cctctgcgtg gcgcgaacaa tgggatcgtg tgaagtagac
     7501 gcgattgtga agctggccac agtacttgtt gcatacaatc cagtaaaacc gtagtaccag
     7561 agcgaagcgt gagtcttctc ttcatccagc gagtgtacaa tccaatcgat cttcgcgatg
     7621 ctctgtgtat ctgaatgctc cacaggttcg gcgtgaaacg ggtgcgccaa acaaaccgtt
     7681 aacaaaacga gtgaaatcca gcggaacaaa gtcatgattg tctctagaag aagcaatcga
     7741 tgacgccaca cattttataa attttgaaaa aggattgagt gcactcagtc gtaacccacg
     7801 aacgggcatg gaacaacgcg gaaacagaaa gtggctggtg ctcgcggcat ttgccggaac
     7861 agctctcgtc agccagatgc tatggttgaa ttttgcaacc atcgcaatca caattcaaaa
     7921 tcgttacacc gtagacgaat ctacagcaaa tcttctgact ctcgtttttc cccttctgta
     7981 cctggttttc tccattccgt caggcattct gatcgatcgc aaaggatatc gtgtttctgt
     8041 tctgtggggc tccttcatgc aggcggccgc ttcctgcatt cgcatctacg acgaatcctt
     8101 ctacgctttg ctcgcaggcc aggttggaat tgcggtagct cagcctttca tcgtaaacgg
     8161 aatttccaaa ctggcggcgg actggtttga cgaagatgaa ggagcggcgg ccaacggaat
     8221 aggaacagtc ggaatgttcg caggaatggc tcttgcactc ggactgactc ccgtctttgt
     8281 agaacactgg ggactgagaa ccgccatgat catcttttct ctggtctccg tggcagcggc
     8341 agctatctat gcattgatag acaggaagtc catttccgca tctaaagaat ccacttcgag
     8401 tatttctttt gggccagttc tcaaagacaa acgcctgcaa cttcttttct tcctggcgtt
     8461 cctgggactg ggcttcttca acggtctcac tgcatggctg gaacaaattc tagcacccag
     8521 agggatcagt gccattgacg ccggaatcat tggaagtgcc ttgattgtgg gtgggatcgt
     8581 tggcgcggcg attgtcccca atttttcaga taagttcaag aagcgtaagc cattcctgct
     8641 ttcgtgcgtc atcgcagctg tctgcctcac gtatcctctc ggaatgggaa gtcggttcag
     8701 cattctcgtg actctggcgg cagcactcgg tttcttcttt ttacctgctt tcgcgctttt
     8761 gcttgagatg tgttccgaag tggcaggcga aaagcatacg ggtgcggcca ccggcattct
     8821 catgctcacc gggaacgcag gaggagtggt tgtaatgctc gcgatggatg caattcgctt
     8881 tggttcaccc tcttacgaca atgcagtata cttgcttgtg gttcttcttg cgatcgcgct
     8941 cttcgcgtcg gtgtttcttc cggaaaccta cggcgcgaaa cccattgaag ataaatgata
     9001 tttttggttg caaatattta gaactcaaat agcgtaaggc cccggcaaac ggggttttat
     9061 gatgccaaac accacacttt ctcgcgcgta ctatgcactg gccatcgcat gcattttgga
     9121 tgtgatcgcc atagctgcag gcgcatctat tccggatttt tatggtcgcc ctgtgctgct
     9181 cctgtcattc gcagtggtag tcttcaagaa cgcggcacac aactctcaca ggctgatttc
     9241 gcttggaatg gtggccgcgg ctctggtaga agcaatgcaa atgctcggca ttggatttgc
     9301 ctggattgca tctgcaactg ccgtttatta cctcgcgtat tccgttgctt tcgctttgcg
     9361 aggtggtaag atcaaaagta aactggaagc caccggattt gcagtctacg ccctctactc
     9421 catcgtcatg tttctgtggc tcgaaccgta tggatttctt cgcatggcct ccattctcta
     9481 cactgcagtc gtaatcctct tcggcggtct ctcttcgcgt ccggtcttcg caagaaagga
     9541 aggagccgcg tggtccagac tgctcgccgg cgcggtggct cttcttgtga tttcagacag
     9601 catgcgggcc atttctatct tcaaagagcc tgtgtctgaa gctctgattc tggcgcccta
     9661 ctgggcagga caatttggcc tcgcactctg gcctattttc gataaaaaaa agcagttata
     9721 aggggcatat aaggggaaag taagcgaatc gcaaggttcg cgtttatgct gagaacatag
     9781 cagggggaat ttactgtgaa caagttcaag acaagctaca caatcgggct gttgctcttc
     9841 gccatctatt gcggtgcaga caaagagaag gggcccatga tcgctccgat tccgggagcg
     9901 agtgcatcca accacaatga tctgaccacg gccggggcct ttaccggtac ctgtttcact
     9961 gcagataagt tcaacgttgg aaggggaatc tacgacatca ctggatctgc ggccgaactc
    10021 ggcatgatgg gctatgccat ggtagatcaa aaaacagctg gaatccacat gagactgcgt
    10081 gctcgcgctt ttctcataga aagtccgtgc aacggcaaac gcctggtcta cgtgagtgcc
    10141 gatcttggac aggtcttcca atccgtcaaa cagggtgtgg tcaagaaact cgttgcggcc
    10201 tacggagata tgtactcaga cgaaaacgta attctaagcg ccacgcatac ccacagtgga
    10261 cctggcggat actctcacta cgcgctctac aatctcacta tccttggcta tgaccagcag
    10321 aactacgacg caatcgtcaa tggaatcttt caggccattg ttcgcgcgca caacaacgca
    10381 aagccggcaa cgatcgaact tgcgactggt gatgtaaacg atgcaagcat gaaccgatct
    10441 ccgattgcat acgctgccaa tccagccgca gagcgtgcga aatattccgc ggacattgac
    10501 aagaagatga ctctgttgaa attccgtgga acagatggaa aggaacttgg attgctcaat
    10561 tggtttgcag ttcacgccac cagcatcgga aacaagaatc atttaatcag tggcgacaac
    10621 aaaggctatg ccgcctatcg tttcgaaaaa gcgaaaggca caaattacgc atccgctgat
    10681 acctttgttg ccggatttgc aaatgcaaat gaaggagacg tttctcccaa tctctggggc
    10741 gttccagatc tggttcacga ctttgatcgc acatacacaa ttggaaatag acaatacgag
    10801 actgcgaaca cactctacaa tcagggtggg aaagttcttt cgggcggagt ggattacaga
    10861 catgcttacg taaactattc gcaggtgact gtggatgctc agtggacggg cggtgctgcg
    10921 aaatccacgt gcaaagcagc catcggcgtt tcgtttgcag ctggttccac agaagacggc
    10981 ccgtccggag ttccattgat tacggaaggc atgacgtacg gtggagcaag ctggcccatg
    11041 ttcacgctgg ttccagacga ccaggcatgc catgaaaaaa agatcattct gcttccaacc
    11101 ggtcgcatga ccccgtatcc ctggactcca gaagtactgc ctgtgcacat tgcacgcatc
    11161 ggaagtctgg cgattgtcgc agttcccttt gagtgtacta cgatgtgcgg gcgtagactt
    11221 cgcgatacgg ttggcgcaga gttgaaggca gacggagtta cagacgtagt gattgccgga
    11281 cttgcagatg catacgcagg atatgttgca acgaaagagg aatactcagt gcaacactac
    11341 gaaggtgctt ctactcattt cggaccaaac cagctcgggg caatgcagca ggaatacgcg
    11401 aaactggctc gtgccatgcg aaacggagaa acagtcaatc ctggccctac accgcgcgac
    11461 ctgagcaata agcaatcatc gttgatcacg ggcgttgtgt tcgatgatgt accgctgttc
    11521 aaaagctttg gggatgttta ccaggacgct gcggcttcct attcgcgtgg tacggtggcc
    11581 agtgctatat tctggggcgc tcacccacgc aacaatttgc gtactcaagg aacctacctg
    11641 gaagtacaaa gatggaacgg ttctagctgg gtcacagttg catccgactg ggatccagaa
    11701 acgaaatacg aatgggctcg agatggaatt gcgaactcaa aagtaacagt caaatggtcc
    11761 atcccggcaa cggcggccac aggtacatat cgcctgcgcc agtatggaaa ctggaaatca
    11821 ggctggacgg gagccattag cgcctacacc ggaacgtctc gcagtttcac agtcaattga
    11881 gtgcagttag tgatttacag tttgccgggt tccgaacagc ctttcgttcg tggactccgg
    11941 gaaactggtt tcagttctga ccattgacga ctctgcgacc gtaattgcct acctccgtac
    12001 tctgctcaaa gaaagacaga tcatgacgga cggatgcggc agcctcgcag aggctcgttc
    12061 cattcttgct caaaagaaat tcgatctgat atttttggat ttagttcttc cggatggaag
    12121 tgggatcgac cttctaaaag agattcgcgc gaaagacaaa accactccca tcctcatgct
    12181 caccgggcgc ggagatgtga agaccgtagt ttccgcaatg gctctgggcg cagatggttt
    12241 cgtggaaaag caggatttgt tcctggatga tttcatgctg gccgtggatc gagcagtgga
    12301 acatcgacat ggaattcttg cacgcgaaca attagaacaa ttgaagagag acatgtacgc
    12361 gatgatcacc catgacctgc gcaatccaat tggaaatgcg ggtgctgcgg cagatcttct
    12421 gctcaccatg acgccggatg accccgacca caaacaagtc ctgagcatta tacgcacgtc
    12481 catcgattcc gcactcggac tgatggaaca atatctagac tatgaaaaaa tcaatgccgg
    12541 ctttctcgaa ctacataaga gcaaatgcaa tctcgcggat ttggtcacag caagcgtaaa
    12601 caacgttgga atggttgcgc gaaaacgcaa acaggaactg accgtggacg cacaagcgtt
    12661 tgaggcagca gtggatccac tgcgaatgca gcaggtgatg gagaatctga tctccaatgc
    12721 gatcaaatat actcaagagg gtggtaagat ttcagtgcga ctgtacggga ccaatggatc
    12781 cattatcttt gaagtagaag acaacggctt tggaattccg cccgcagaat tggaaacgat
    12841 ttttggaaaa ttcaaacagc ttacgaatgg aactaaactc tccagaggtt ttggcctcgg
    12901 gctaatgatc gcacatgaaa ttgtagaagc tcacggagga accatcaaag cggagtctgg
    12961 tggctcgatc gcgaagggct cgcggttcac cgtccgatta cctgctgttt gatgaagagg
    13021 gctggattca gacggcgaca gaatcgccgc cacttctcac ttccctgtga tgttaaatac
    13081 gctcttgttt tcttccaccc agcgaattag cttttccaca ccttcgcgag gcttaacctt
    13141 tggattgaat ccaaacttac gtgcttccgt gatgtcgcag atgaagtatc gcatatctcc
    13201 aggacgctct ggatgaaatt caatcactgg ctttgttcca agaatttctc cgatcattgc
    13261 gatgcactcg atgagtgaaa tcttgtgttc tggtccgccg ccaatgttgt acacaccagg
    13321 gataggattt ttgaaatacg cgagataact ctgcgctccg tcgccggcat acaggatgtc
    13381 gcgcgattgc tttccagttc caaacactcg caaaggaaga ccaaagaccg cgcgaattgc
    13441 aaaattcgca acccagccat gatcttcgcc gccaaactga cgaggaccat aaatgcctgt
    13501 aaaacggaag cttgccccgg gtgtctgaag catgtcggta aaagtgcgca cgtaatgttc
    13561 tgctgccatc ttggatgcat ggagcggaga aagttgccct accataacag gatgcttttc
    13621 atcaatggca acgggtgttc tttcgtaaga agtggcaccc tctttcagag tgtcgttgat
    13681 tgagtttcca tacacgtgaa tgctcgaagt attcacaacg ggaatcctgt gctttcttgc
    13741 agcgtccaga acgttgaaag tacccaggac attggtgttg aagtccaggc gtggatcttc
    13801 ccaggagatg gtcatggcag gctgcgcggc tgtatgaaca atgaagttgc atccggatga
    13861 atgatctttc atggcctgct cgtcgcagac gtccgccttt accatttcgc agccaagagc
    13921 tttgaggtag tcccagttaa aatttctggt cttttcggtg ccgtatccgg tcttcgcaag
    13981 ctcgtatttt gtcatgctat catagctaac aacttcccag ccttctttgc gaaaaagctc
    14041 acacacatgc gaaccaagaa aaccacatcc accagtaacc agcactttca tcgcataact
    14101 cctattagtt gatcaagaat cagggcgcca gcagcgcttc tagatccttt ggaacggcgc
    14161 atgcaagcac gttcttctcc acgccgagcc cctgcagagt atcctctgaa agtcgctggc
    14221 tcgtttttcc tggcccgaag tcaacgatcg tggtgatctt gctcgagtct gcaaccggct
    14281 tcatggactt atcccagtaa aggggattga ttgccatatc cagatacatt ccaatcgcaa
    14341 tgtctgcgtc tccctgaaga tttcttccat catagaagga atacacaggc accttgagcg
    14401 cgctgcccgg ataagagaag ccaatgcgct tgatgtccgc ttcaaacaat gggcgaattg
    14461 ggtccatgag agggcagtga aatgggcaac tggtacgaag gtaaactgcc ttcacttttt
    14521 tttcagcaag agccgcaccg tgcatttcag tgaaacgaac gagagaggca cgatacgaag
    14581 atagaatgcg gttggacggc gaattgtaga gactcacata aatcttctga tccgctggca
    14641 gctctttgtt ggttgcatct accattgcct gaatggtctc gtgcgaatct cctacaaccg
    14701 ccatcatggg cgctggagct ttgttgcctg ctttttcaga tcttgcgatc tcatctgctg
    14761 tgggcgcgaa gaatggatat acttcctgcg cgcgcacacc cagatatagt aagtatttca
    14821 catattttgc cgcggcggcg tagtaagcat ctccatcgag atccagtgca accagagacg
    14881 caggaatgag gccctggctg tgaccgctgg ttgcttctgt ggctgcaacg aggtccttgc
    14941 gagagattcc cttgttcact gtgttttcaa aatgagcaag ttgtgtaacc tgaatcatgg
    15001 gaatcgacac agcagcgcac gcaagataat cctcagatgg aatctttgat tcatcttcga
    15061 gccaggcacg tgcgtccaga ccatgcggca gacccactgt cccatccaca tgaggacgct
    15121 cttcttcaat tgcgcgaagc acaacatcga agaatttctt catagaaggt tctttataat
    15181 attttgcaag ttccttgtac cagggcgcac cctggccgcc aaactgaaca aacagccggc
    15241 cctgtccggc tttggcctga gaaatgatct ttgacatata cctatcctta cttgcttacg
    15301 cgttccacga aggaaatttc gcgcaggtcg atcttgatgc gatcaccctg ttttacatac
    15361 aatggcacaa gtacttcccc gccggtctcc acggttactt tctttcgtga agttccaacc
    15421 gtgtcccctt tgatcgcgtc ttccgcatac gtaacttcta gttctgcaaa gttagggggc
    15481 gtgacaccaa ccggattgcc ttcgtagaaa gtgatttcaa aaggcatctc ttccttcatg
    15541 aaaggaacga tgtcttccac gagcaccatt ggaatgctta gttgctcata gtcatctttg
    15601 tccatgaaca caaggtgatc accgtcttta tatagaaaag tcatgtagcg cttttcgaga
    15661 tcaacatcgt cgatcttttc aaccgctttg aaggtacgct caactacagt cccacgcttc
    15721 aggtttctga gctttgtgcg gacaaatgcg ctgccttttc cagggttgac gaattgagag
    15781 aaggtaacag tgtagaggtc gttgtctact ttgatgacct ggcctttacg caggtcgatt
    15841 ggatccatca tgcgaatcga gatttataaa aaaggcaggg cagtcaaaca gaattacgat
    15901 tggaaggaag agctgagatc tcgcatcagt tcggcggatg aactcctcga attcatgccg
    15961 gatttgccag aatcgccgct ctttcgccgt ttgtatgcat ctgttgaaga tttccgaaaa
    16021 gtggcggaag ccatggcggg agagtttcga ttctctgtaa ctccttacta tctgtccctg
    16081 gccaatctgg agaatgccag atgccccatc ctccttcaaa tccttcctcg ccgggaagag
    16141 ctcgaagacc aggtgttcca gttcccggat cctctggcag aagattctca tcatcccgtg
    16201 gacggcctca ctcatcggta cccggatcgt gtgctctggt atctttcgca caactgcgca
    16261 gtctactgtc gattctgcat gcgaaaacga aaagtatcca ggtcaaacag cgcaccggat
    16321 cggtcttcgt attctgcggc acttgactac attcgcaatc acaaagaaat caaagaagtg
    16381 atcctgtccg gcggagatcc actttcgcat tcggatcaaa ccattcagtt cttgcttacg
    16441 gagcttcgca caatcagcca tctctccagc ctccgcatcc acagtagaat gccggtcact
    16501 ctgccgtctc ggattacaga tcaacttaca gacgttatgc gacaggcctt ccctgttact
    16561 ctggtgactc atttcaatca tccagttgaa attacggagg aagctacctc ggcagtgcga
    16621 agattgagaa tgaacggtgt gaccgtattg aaccagacag tcctcatgcg cgatatcaac
    16681 gactccacac agacaataga agaattgatg ctaggcttgc tccgtatagg cataaagccc
    16741 tactatctgc atcagtgcga tgaagtgcac ggggtttcgc actttcgcgc gaacatagaa
    16801 actggtttgc aaatattgcg cgatcttcag ggaagaaacc cgggcattgc aattccgcgc
    16861 tacgtcattg atcttcctgg cggcggaggg aaggtgcccc tggagaatca atacctcagg
    16921 cgcaggacgg atgatggcct ggaatttgag aactggagcg gtttgaaatt cactacagtc
    16981 caggatcgcg aagacaaata aaatgctcgc cgtgtgcccg ggcccggtcg acttctagta
    17041 cgtgaagcag tcagaactat cacagaaact gcagatcaag tctggcatgc ggattgggac
    17101 catgaatgcg ccggatatgt ttgccagaaa gctcgcgccc gttccagagg gatcggaact
    17161 ttcgccggtt gcaccctatg atcatctgac tgtatttgtg aatcgcaaag cggaactgga
    17221 tcgtttcgct cttggtgcga tgaaatctat gcgcccgggt ggaattctct ggtttgccta
    17281 tttgaaaaaa gcatcaggcg caaaatcgga cgtaacgcga gacgatggat gggacgctct
    17341 tacgcgggca ggatggggcc cggtatctct cattgccatt gacggcacct ggtctgctct
    17401 tcgcttccgg cccctcacta aggaagaaca agaagatcgt gccaaccgca aggttcctct
    17461 ggacgaagga atagaaaaaa agccatccaa acctaaaccg agcgtcgcac gaaaacctcc
    17521 cgcagtgccg gccgacttaa cagcggccct tgccaaaaat aaaaaagcta aacaattctt
    17581 tgaaagtctg gcgccatctc acgcgaaaga gtacattgtc tggatcacag aagcaaagaa
    17641 ggctgccact cgcgaaacta gaatcgccaa aaccatcgaa aagttgctcc aaaaaaagaa
    17701 gaacccaacc ataaaatgaa tctacattgc atcctgctct cgggaggaac cggagagcga
    17761 atggcagccg gaaaacccaa acaattcatc gaattattgg gtattcctgt tctgcgctgg
    17821 agcgctctga cactttcaaa cttttcaaaa cgccgcctga cggtggtagc tccggccaac
    17881 ctccttgcag aaaccagatc catactggaa gggatagaca gcgtagtagt tccaggtgga
    17941 tccactcggc acgattctac gttatgcggc attcgatcca ttgcaaagga aatccatctt
    18001 cagagcgata tcattctcat ccacgatgcc gcaagaccag tcatcagaca cgctgaactt
    18061 tcgcgcctct gtgatctctt caaagacgaa acaatcctat gctcttccct tgtgggtcca
    18121 atcgtggaaa cgatcgttca atcgagggat gggaaaacat ctgatggaat tgcggacaga
    18181 gatcaactcc gcgcggtaaa aactccgcag gcattgcgcg caaatgctct tcgcgcactt
    18241 ctggaagtgg aaggaaattt tacggacctt ttgagctggg cgcacgccac gggattgaag
    18301 gcaggtctgg ctctggcaga ggaaacaaat gtgaaactaa cttctcaagg ggatctggat
    18361 ttgatgttga gcttgctcaa ccgtctgaag atcgacggac cttcgtaaga gcttcctcgt
    18421 actccggttt tcccagtccc agtttcttgc agagagagga aagcatggtc tgttcatccg
    18481 gactgagaac cgcaaattct tctgcgattg cttttgcgtg tcgagggaat actctcttca
    18541 tgagtttctt cccttcttct gtgaggtgaa cggtaatgta gcgacgatcc tctgtgtttc
    18601 gaactctttc cacgaggccg cgcttctcca ggttgtccac aatcagagtg atgttcccgc
    18661 cagtggaaag ctgctttgct cccaattctt tctggcacat aggacccaga tggtagattg
    18721 tttccattac accgagttgc ggctccgtga gtccttcaag gtgcatggaa ccttcgagcc
    18781 ttgaagcaag agacgaagat gcacgcgcca gtttgatgaa cgcgtcgagt gctctgactt
    18841 ctgcgtctga tcctttgtaa tgcgttggca tagcgcgctc ttgatcttaa gatgctacaa
    18901 aaagtcaaaa tgtcaaggtc attccgactc gaaacgagaa atgtagcgac tgaacgaagc
    18961 gaattgctcg cctcaatctc agaattctgc ttctttaatg cgatgaacat tctcgtcatt
    19021 ggcgcttcca aaggcactgg agccatctgc gtgcaaaacg ctctcgaacg cggtcattca
    19081 gttactgcct tctctcgctc tcctgaaaaa cttgctctca accactctcg tcttcgcctt
    19141 tacccgggca gttttcatga tgcggcaaac gttcaagccg cagtgcccgg gcatgacgct
    19201 gttatcatca cagcttctgc atcctcgatg caggcgttta aagaaaatcc gaactacttc
    19261 tcaaatggaa ctgcaattgt tatcgatgcg atgaagaaac atagggtgcc tcgcctggtc
    19321 gttctttctg ccctgggcgt gggtgagagc gggaagctca tgccatggcc actacgaaag
    19381 atcgtagtat cctggcttct caagcttccg ttcgcagatc acgaaagaca ggaaacaatg
    19441 gttcaatcca gtggactcga ttgggtgatt gcgagaccag gacgtctcac agatgggccg
    19501 gcaaaaaaac aataccagaa gaaatctcaa atagaatcga tcccaacttc catttcgcgg
    19561 gcagatgttg ccgatttcct ggtggaagct tgcgaacccg gcgaatgggt gcgaaaagca
    19621 gtacagatag gcggctagtt atcccacgac agcttccgct tttcgcgaac gcggtgcggg
    19681 aaaagaatat tcaagtgcgg acaactctgc gatctctttc atgcgctcgt gcaagaagag
    19741 accgtgatcc atgtctgcgg tgaagtaacg cgttggccca aagcgaatca caacgctcgt
    19801 tctaggaaat cctggtttgt aacgaaatcc aactgaattg caggcaagag gtctggctgg
    19861 tttgccagca aggaatctaa agcccgcgag ctttccttct cccatctgtc gccagtaggt
    19921 ggtttgctcc ggaaataaaa ccatcaagtt tccatctccg tctttcaatc cagtgatgtc
    19981 cttttcatag agaactttgc gcgcataaac gagatagttc ttactctgct gaggatcttc
    20041 tctctccagg ggaattccgc ctatcttcca gaagaatccc agataacgag gacgaaccag
    20101 gctcgccttc atgatgcacc aggcatgtcg cccgaaagcg cgcttcatcg catggtatcc
    20161 gagtggaatg tcaaattcag cgctgtgctt ggatagaatg agtacaggtc cgttcttctg
    20221 tgcgtcagga tcgatttcga tcttcacctt gaagaagaga attccaaaca gaactccaat
    20281 gaaattcaaa atgttggaag cccagggttt ctttccgggg tgggcactgt aatcgaacca
    20341 catcgtgaac gatcgcgcga aatgcgcagc gcggtaaacg aaaaatcatt gagcatccct
    20401 acaatgcacg aatgtgtggg gctggagata tcggtgaaaa tcccaatgta tcaggtagat
    20461 gcgtttacac agaaggtatt cgcaggcaat cccgcagcag tctgcccact ggaaacctgg
    20521 cttccggaaa gcacgatgca ggcaattgca gcagagaaca atctcgccga aactgcgttc
    20581 ttcgcctccg ctgcgaatga ttttcaaatc cgctggttca caccggaggc ggaggtggct
    20641 ctctgtggac acgccacact tgcatctgcc gttgtagccc gacatctgaa cctgttccag
    20701 gacagaatct gttttcactc caaaagcggt cctctcttcg tggatcgaaa agacgatctc
    20761 tacactttga attttccact gcgcacgatt ggcccggaga ttcaagtttc tcccgccatc
    20821 gcagagtgcc ttggaacccc tccggtcaga ttattcagat cagaaccaaa ctatcttgca
    20881 gtcttttcgt ctgaagaaga ggtgcggcgt ttaaatcctg actttagaaa aatgaaagag
    20941 atagcgggac atggaatcat tgcaacagcc agaggcactg aatttgattt tgtatcacgc
    21001 ttctttgcac ctgccattgg cgtagatgaa gatcctgtga ccggctctgc acactgcacg
    21061 ctcatccctt actggtctgc ggttctaaac aagaccagaa tggtggcgta tcaagcatcc
    21121 agaagaggcg gagtattgaa ctgcgaattg aaagacgagc gagctctgat tggcggatac
    21181 ggtgcactct attcagaagg attcattttc ataagtgaag cggggactcg atgaagagga
    21241 ttgtttgcct gacacttcta gtatgcgcaa cagtttcgca cgcacagaac gttccggccg
    21301 gtatttttca cagcatacat ggggaggatc catccaccga gaatgaattt tatctttctg
    21361 tttcaagaaa aggaacgtta accgccatta ctctgatttg tgcgacgtca tgcctgccca
    21421 ataaaccaaa accttacgtc ttttcagctc aatcctacaa gggcgaaaag atcaaacaaa
    21481 ttgaagcgaa tgtatggcag gttgctatga aattcgaatc ttctgcaaat cgaaacgaaa
    21541 aaatgcataa gccctttgat cttcgcctcg atctgaatgc gaaaaccatg tcctttgatg
    21601 gcaaaactat ccctcttgaa aaattcaatt ttcaatccgc gtcctaccat acgatgggag
    21661 actatggatt tgaatctgaa atgctctttg tatccggcaa ggggaagaat cgcgaattct
    21721 tgatcaagac acggagcgca ccggaacacg aagacgaatt tctatatttc acatcttcca
    21781 gaaatgaaat ccatcccatt caggcgactc actggcggta cgttctagat ccaaaagccg
    21841 gcggggaaac tcacttcgcg cagctacacg cagacgttgc cataccagga cagggcccgg
    21901 cccaaatcaa actcgatgtt gtgcgcactc cagcaacgga tgtgggcttc tggctcttgc
    21961 gcaaaaacaa atctccaacg cgattctggc aaacgaatga ttcgaatact cgcagatttc
    22021 ggttctgcgc gaattcctcc gccagtgacg aagctggctg ttctgttctc acgatcgcaa
    22081 acggaacggg gaaccctgcc tgctccggtg ttgaaattgg aaccatcgtt gtaatgctga
    22141 accaggggaa agcgcagatc ctctcagacg atcagaaggg acatctgctt gacgaagatt
    22201 gcaaggtcgt ttactcgttg gaatatcggg aagaaataaa cgccgtctcc atcaaaggat
    22261 ctgaagggcc ggcaaaggta ttcgtggaaa aacagccaga tccctgatcc cagaagaaac
    22321 agaaacgaac tcattcaaaa tgaaatggat caaaacaaca ttcttgcttt tggcactatg
    22381 cggtccactg cttgcagaac ccacagtagt acgcggatca cgacttctcg atatcaaaaa
    22441 atccgggaag atcaagatcg ggattcaaaa cgaatacaga ccctttcgca ttgaaaatcc
    22501 aaaaccagga tatcctggca ttgatgtgga actggcaaac gcactgggag aagctctgga
    22561 agtagaagtt gaaatttctt atctacctct ggaccagctc ctgatagaaa cgaacgaggg
    22621 taagctggac gtaagtcttg gtggaatctc ttctacactg gaacgtgcgc gaaaagtaaa
    22681 tttcagcgat ccgtatatga agacaacacc cgccggcctt ctggcgcgcg attctttgcc
    22741 cccggaatct gaatccgtaa attatgcgcg cagacagttc aaaagcctgt ctgacctgaa
    22801 atacctgggt ccgatcacaa tcggagtgcg cgcaggtact gcaaacgaaa cccttttgag
    22861 aacagatcct gatttccaaa aacacaagat caaagtcttc caggatagaa ctatagccct
    22921 ggcggcactg gaatccaggg aaatcgatct tctggtcgcc gatgacgtgt atttacgcgc
    22981 tcttcttctc aaacgaccag agttgatgaa tcgttttctt ccactgctgg acaagtatct
    23041 ggaagaccac attagcatgg caattccacc aggagatcca gagctctgga attacttaaa
    23101 cttctttatt aaagaaatga ttcgtacggg gcgaatgcag acaatcatca acaaatattt
    23161 tcagagcggt gcgtggttct agcaaatgcg taggatttca ccatcccgcc tgacgggtct
    23221 ggcgctggtt atcgcttcct ctttgattct tgccttttct tcgcggcccc ttcatgcgca
    23281 ggaatcaagc acaccgatcg cagatcgttt cagctatcgc ggagatatgt tcatacgcgg
    23341 gatttatctg gccagagacc tcccgctcaa gtcgcagtcc tacaatacaa catccagaaa
    23401 tcgagaagaa caggatttct attccgccag actcagattg gatctggctt ttcgcgcatc
    23461 gcaatatgtg gacgtactct acggccttga agtaggcgaa ctaacatttg gaagagacaa
    23521 tgcgtcgaga gccggacctg gatcaggtgg aaagggtgct ggagctgcga acctggagac
    23581 cagggaattg atcctaaaat ttcgtaacta tccagaatct ctttcattca gcgcgggaat
    23641 tctaaacttt gatacaccca ggggactggt gacaggcaca agcggagccg gtgcaaaatt
    23701 ttcagctgaa atgcgtgccc ttcattcatc tgtggacgct tactacattc gatctgagga
    23761 caattccacc ctggacggag attcaaatgg tttttcagat tcaaattttc gcaacgtcaa
    23821 tatgggactc atttcctgga aattttccgg aattcccggc atgcgaagcg aattctacgg
    23881 aatgtacaga caggacgatg acccatctct gactgcagga gacagcggcg aaacatcgag
    23941 agtctactgg ggcggaatat tcactcagta caaggcagga cagtt
//
LOCUS       DAOQJL010000002        23639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_952_length_23639_cov_4.740629, whole genome shotgun sequence.
ACCESSION   DAOQJL010000002 DAOQJL010000000
VERSION     DAOQJL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23639
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_23639_cov_4.740629"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLG78_00140"
     CDS             <1..205
                     /locus_tag="PLG78_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21542.1"
                     /translation="DRHIRFAEGFHRRWMGSNEAKLKWCERVSSMKINMLCPQHGAIY
                     TGENVARFISWFAELPVGSGNVP"
     gene            complement(255..1610)
                     /locus_tag="PLG78_00145"
     CDS             complement(255..1610)
                     /locus_tag="PLG78_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21543.1"
                     /translation="MILRMSLNICLTGIGTSQIWPRVPQSGTKRRIHDLQKIHARRWK
                     RGFDLLWLHISLEAQNPTEEDKRNNVTQPAPATPAPAPDAIPDGAVDVQLKFTSNFLD
                     RGEDRGRNRSVQRGKAYNSKEDPWFFQPSAKIKVPGSGLYFNAAGSFALSNRNDKDVD
                     QKLEDGPTGEQTLLTDSVNPLVNTARLPASTYIVDQLTMLPTWPTTPIHSLSVYKMYK
                     DANGLKRGDQIDLSFGHQSETKLGNFDFGINASTRTDPKGKSNPFGDGGANYQKTEMY
                     VTYAPPFLTELSFTILADVVNSDQYMKLAYTKEFEIKENLKADVSAGLGYMSLGTSGQ
                     SDSFHGLADSPFGAGISWNGFRFGLEATYRHSYDIYDSQVDGDPRTSRALYIDGGSTA
                     GDGLVTDPSRTKGFGNQLVNSLITSRVRAMTNNTSYTYTPRQKVPRWLYAVNVSYSTS
                     L"
     gene            1669..2820
                     /locus_tag="PLG78_00150"
     CDS             1669..2820
                     /locus_tag="PLG78_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNE21544.1"
                     /translation="MQKQLLIIPQAILITISCLKPQPVSEAAVVRDYSLLVHASYLQA
                     RKDAQAMQKSVSDFLAAPGDAGLKSARDSWIKARVSYSQTEAFRFYGGPIDRPKSQGK
                     EEGPEALLNSWPVNESYIDYVKGNPGAGIINDPRIQINATTLTGKNQQKDEADVATGF
                     HAIEFLLWGQDFNPAGPGDRPVSDYTTKSNHERRKQYLRVTTDMVVTQLRSLEEDWAP
                     GKENNYAAEFQKLNSKVALGHILTGLVTLAGFELASERTATALKSGDQEDEQSCFSDN
                     THNDFINNIQGILNVYEGRVGDYSGIGLDAWIKSASPEMADQILEALRSTKDKAISLP
                     VPVDSILASPKNSDSRQKLESLSHALAQDAKLFQKAGILLGVDARLIAE"
     gene            2829..4208
                     /locus_tag="PLG78_00155"
     CDS             2829..4208
                     /locus_tag="PLG78_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNE21545.1"
                     /translation="MCKGFPIVLVLLSLSECRRARDPGLQYSGGETTVFVTSSLAFSS
                     PAANLDVDKMPAFTFGNRVFNTNWVTAPSSTKNFDGLGPFFNRVSCSSCHLLDGRGQP
                     PETPKDTMDSMLVRVSLPGESEVGGPRPHPIYGDQINDRAILGVKPEGRITVRYVEIA
                     GNYPDGKHYDLRRPVYSFRLNYGDAPGILFSPRVAPAIHGLGLLEAIPEAAILAIARL
                     QASSGIQGKPNYVFNALTGGKSLGRFGWKANVPTLRQQNAGAALGDIGLTSEIFPREN
                     ITAIQKDNKALASVAGLGGDSVDLKTEFLDDLTFYTQTLGVPARRNHKDPEIRKGEQI
                     FSQIGCSGCHVPTFETGEHPAGIKEVSHQVVHPYTDLLLHDMGSDLADGRPDFQASGS
                     EWRTPPLWGIGLVEAVNRHTTLLHDGRARNLEEAILWHGGQAARAREAFKNMSEKERT
                     LLLRFLKSL"
     gene            complement(4224..5270)
                     /locus_tag="PLG78_00160"
     CDS             complement(4224..5270)
                     /locus_tag="PLG78_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE21546.1"
                     /translation="MNTRALLLKVAIFVTLSAAVACKKDQVDLQGAAITALQGSVSVT
                     DNKGTNKTISPSSLYTKDGILMPGYVIQTGRDGQVDLQFSPTMKLRMGPDTTLKIESA
                     RILASKGFEQLQLRLEKGRVFLASEKLAAASRINVVTPTAIASVRGTEWVVKEENGKS
                     QTMVKEGKVEVADDTLKDSQVVEENKKAEVQSDGKITVSTQTDSDKKEVADLGRDLAS
                     ITENGRNEIQSLIQQFQEQKILIQQTIEEQKKSNEEIMQQQKDSNQELMKDQHKKDLE
                     NLREQIQRDREEMDKLKSQLGADKDAMTQGGREEKDKISNQGRDEINQMKQNSPLSTQ
                     DQNKAELERLRKGQ"
     gene            5370..5882
                     /locus_tag="PLG78_00165"
     CDS             5370..5882
                     /locus_tag="PLG78_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNE21547.1"
                     /translation="MKKILTFVDDIYEDLELWYPKLRVEEAGWSVDLAGPEIKKYSGK
                     HGYPASSNIKISDVQEDYYQALLIPGGFMPDKLRRDARVLSITKNFFDSGKLVAFICH
                     GGWIPISAKILNGKKATGSRGIKDDLENAGAIWVDEPVVRDGNLISSRTPVDLPAFGR
                     AITEYLADHS"
     gene            5879..6511
                     /locus_tag="PLG78_00170"
     CDS             5879..6511
                     /locus_tag="PLG78_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNE21548.1"
                     /translation="MKDSYLRLLENNKIWAAEKIKENPKYFQHLAELQTPEFLWIGCS
                     DSRVPANEITGTDPGEIFVHRNVANLVVHTDLNMLSVLQFSVEVLKVKHVIVCGHYGC
                     GGVKYAMDTQDHGLVNKWLRNIKEIHVKYDEQLSKLTDVRARADRLVELNVIEGVRSL
                     AKTSIVQKAWKTRSLQIHGWVYGLENGLIKDLNVMVNGIADLEPIFRLDV"
     gene            complement(6685..7350)
                     /locus_tag="PLG78_00175"
     CDS             complement(6685..7350)
                     /locus_tag="PLG78_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE21549.1"
                     /translation="MANMLDFQFEGMRLKYIKKGSGRPLLLFHANGFSAGSYAPLTDH
                     LANHGFHVHALNFMGHGGSDSTTDFHNWEFFGRQAAAFAEHLGYERFFAVGHSIGGAS
                     LLLATPSMEGRLVAGALLDPTVFSPLTSRFASLIPNPLAALAEKRRREFKDLRVVSRS
                     FRMNPLFKQWHPASFEGYLETAFEATNAGYRLCLDQKLRRKYFEAFMRASGESFALSR
                     NPF"
     gene            7518..9542
                     /locus_tag="PLG78_00180"
     CDS             7518..9542
                     /locus_tag="PLG78_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE21550.1"
                     /translation="MNQSDNAENVLERILSACIHATHAESGSIMLIDEDGENLTIHVS
                     QGLAPESAHLKLRIGEGITGNVARTGKARIVGDATSESDYIPVKEGLISELAVPMAVR
                     GEVIGVISVDSSGKNAFDRDHEHFLSIMSNLAAGIFVNIKDNQLLKSRDRFHTVLHEI
                     SRIVSASLRLEDVFREIMTITEKAFKLHRSTLLLYDRQQDVLAIVASVGFSKDELVDV
                     TYAPGEGVTGSVFVNKKPVYIPSVSNEPGFLNRLKTYGETGDMGFFCCPIFYGTEVVG
                     VFSTFTRQQGLDAESMIEFLEILGSMISQSITIQRLVKEETRVIASENIELKRELGSR
                     YKFGSLIGKASSMIRLFEKVRIIADSRASVLLTGESGTGKELIASAVHYNSPRRDQPF
                     VKINCAAIPENLLESELFGHRKGSFTGAIADKKGKFETADGGTIFLDEIGELDLNLQS
                     KLLRVLQEREIEPVGGRTRQVDIRIIAATNADLEAGIADKKFRADLYYRLNVINLKIP
                     ALRERKDDILLLVNHFIEKYTKENGKSLTGISHAALKMLEDYEWPGNVRQLENAIERA
                     VVLSQNKILDVDDFADTTLPFSAPPQNAGVPAVQAVAVSSPGALELPDEEQFHHMDGH
                     VYDSVIQEVEKRLILHALKKFKYTKTQAARYLGINRNTLDKKIRELSIDY"
     gene            complement(9664..10128)
                     /locus_tag="PLG78_00185"
     CDS             complement(9664..10128)
                     /locus_tag="PLG78_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE21551.1"
                     /translation="MKAEYVNPFLQGAVLVFRDLLQEEIIRGRTLVKKTPAPSHEIAI
                     LLNVTGSVHGEVIYSMNAESAYRIAEKLMPGTDRSSIEKEYRDVLGELGNMITGNALN
                     IFLNSQKDLDMSVPYVIDMRTQTLKVQDKTTLGLHLYSRLGMLELNIALQES"
     gene            complement(10167..11375)
                     /locus_tag="PLG78_00190"
     CDS             complement(10167..11375)
                     /locus_tag="PLG78_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21552.1"
                     /translation="MMRVWPMGSVDIIKKFGSVLIIVILGVMLALTFSKTPLSTITDI
                     LSGAGRVGSFDGEEIQPRIYLMAEDNCRQNMSAFGQIPEYILKNCIANQIRQLYVLPL
                     LAHRLGVAPSEKFMQDELMKGAQEMYEAQKNAAPDDRLTVKEIYHKQLASFPMDIRLR
                     MRAAQQAYEVISSMGEALPTARAEAAADSISMNARIIRFTTLDLTEKSGGNIQVTEEE
                     IKAKHEKEQSIFEPAQRKSYESQKEFVKNRVLTEKRQLASAGLKQQLSQLGKDFKLED
                     IERITGKRASQVNIKLSQLKGVALPSGETVKLDGPEVLGALGGDGVKKIGPIQDGEAT
                     VYVEISGVQSVADAGLETRAQKIAEEKRNEAGRLYFELLLKKESERGRFKLKSEVKDT
                     GPVSHPDDSF"
     gene            11442..12098
                     /locus_tag="PLG78_00195"
     CDS             11442..12098
                     /locus_tag="PLG78_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21553.1"
                     /translation="MYFIGIGLILLGLISLAVLFFSVSTERHQEEPGHIMPVVTRAPD
                     LRDESVRPAPLAPVHPEDAPVSHSQAVAEPSAEPSPSILSHAAVRAVSPVAPAVSDFR
                     PAAKRKDPGQLAVFGTLFLDHARNLSLLSSRGTEDLPPRMFSDFKRVGKGSLVCEGEG
                     FFFKSGNSVHAYSTGDLEQIVFLSSGVALVPGSTQSPIAIFLTDESDRVKAFIRENAA
                     "
     gene            12099..13142
                     /locus_tag="PLG78_00200"
     CDS             12099..13142
                     /locus_tag="PLG78_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21554.1"
                     /translation="MRNYESNTSSRKPLAIASLTLFLIGGAIVAVFYGDQVVAFASGQ
                     ILGSADTRIQKRLALFEQEYKKAGRSPAELLDVVESTRKIVQLMEREKPASAEVYYYY
                     SLLNYYEILVRVPLNGPSLLRMTGRGYLPDPPEGMNLAPVSISALAQETASLARKSLA
                     IDPKPAFASRVRAALILGELMATGRTDPNLLQTTLAIKKEELTPLTQQTTDWMRWALF
                     AMQGKSLELEQEFTERKMLVDPVDGSKPDPDSLDANEARLLLSHACFNAKDYIRALNY
                     ARQVKYNPNTETIWKVDAARLEGEIFLVQSGPIAARPFFEEALKLNGGKDKFLEDRIA
                     SLSPTRPVRTQAP"
     gene            13223..14245
                     /locus_tag="PLG78_00205"
     CDS             13223..14245
                     /locus_tag="PLG78_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE21555.1"
                     /translation="MMFDRVFSLFSNDMGIDLGTANTLVYVKGQGIVLSEPSVVAVQS
                     STGKVLAVGNEAKRMLGKTPGDIVAIRPMRDGVIADFETVEKMIRYFIQKVHKRTTFV
                     KPRMVIGVPSGITEVEKRAVRESAEQAGAREIFLIEEALAAAIGADIPIHEPAGSMIV
                     DIGGGTTEIAVISLGGMVISDSIRIGGDEFDEAIVRYLRTQYNLVIGDRTAEDVKITI
                     GNAFPEKKTEVRELKGRDAISGLPRVLEIDSNEIRKALQEPIEQILEAVKSTLEKTPP
                     ELAADIVERGIVLAGGGCLLKGLDKYISKETGVPVFRAPNPLICVALGTGKYLDELKN
                     INRGLK"
     gene            14322..15332
                     /gene="mreC"
                     /locus_tag="PLG78_00210"
     CDS             14322..15332
                     /gene="mreC"
                     /locus_tag="PLG78_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNE21556.1"
                     /translation="MIWQRNPLARTVGFFGKVADRASGAMNSGLSATGRFWVEIDKFR
                     ELEKRYAQAEKQIEEYKLEKDKFDALKKENDTLRKTLGFAIPAEFPEVRAEVLGVRLN
                     SISPRVIIGKGEETGIRPFMPVIARSHDADNNTIRSVVGIVVSADNGTSVVQPLIHPG
                     FQLGVRLPDSGAWAMLSGNSGNPTQALLTVISGDGSLDGKKPIGQVVVTSGDGGVFPK
                     GIPVGTIVKEGPRQGQARTFFVKPFAQLMQLDSVTVVIKKPAAWARKNDEDKLEEFLK
                     TEFGAPEYPETLMRLDSGRDRGPKQTQDPGEQTTGTDQQPATQGQQNEPRRIQNVNPQ
                     TR"
     gene            15334..15852
                     /gene="mreD"
                     /locus_tag="PLG78_00215"
     CDS             15334..15852
                     /gene="mreD"
                     /locus_tag="PLG78_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNE21557.1"
                     /translation="MFLNFAVVAIGILVSFFLKGINFLSLDLESVGGGILYPDFLLIL
                     VIFFALRRGDFSALWVGFFSGLLEDSGLLSFADKTGEFVPIIGTHALVYCLIGFILGK
                     VHKLMDKDRVPTIILIVFVTSVVERIIVWLLVGVLEDFNKSFSVLGPSVYTALFAPIW
                     IAGLSWVYKMQE"
     gene            15853..17760
                     /gene="mrdA"
                     /locus_tag="PLG78_00220"
     CDS             15853..17760
                     /gene="mrdA"
                     /locus_tag="PLG78_00220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE21558.1"
                     /translation="MSRSASEFRLEQQFQTRVYIFTGFMILVGTVLTLQMGNLQLLHG
                     AENRRMAKKFVSRQEFTVAPRGLILDRTTDPANPLVKNLITTDFVVFPSRFENLEEGK
                     GYIREFCRIMGRNYANYEQYLTPEAWKVLVRRNESITLLTRMDTAEHERLGELRMVTE
                     YGSFVTNHLRYYTMGPAAAHVTGYIGLPSRSELDRGLALPYQMLGKGGIEARYDSDLR
                     GTDGVRIRHRFVDEEEQLAESEQGNSLVLTIDRRAQAAAYNSLKNSGRRGAVVAMRPS
                     TGEILALVSYPAFDPNILSSGSADQRSRHTKTVQDYEGFLNLAIQAKFPPGSTFKPLV
                     AIAALENSAENHVTEDTSFHCAGSWLLPSSVAGVPPARFNCWNAGHGTNNLIGAIAQS
                     CTVYFYQLGYKIGPNAMIHFAKATGLDKKTGVDLPGEIQGFVPDQRWKQITWSSRWYD
                     GDTVNLAIGQGFLEVTPIELAVLYSAMANRGKIYRPYLIKEVRDPVSNRVLRQVRPDL
                     IHEVPISQKSLDIVQEGMRAVVSRGTAQHLNRPDYPPIAGKTGTVQTRSRIKAANHAW
                     FASFAPYGAPIEEQIVVVVFVEFGMGGSAAAAPIAGDVFHAVFPDWTPTVHPQIKPDR
                     KEETPPNGKPQ"
     gene            17757..19283
                     /locus_tag="PLG78_00225"
     CDS             17757..19283
                     /locus_tag="PLG78_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE21559.1"
                     /translation="MINRYKLDVYLLGAAVVVVLAGIFTLYTQEYQLAEGSGRWYKQL
                     IFFGLGLIICLGIRRMNYQVLEAYAVPAYLGSLFLLMLVLVFGATIKGAKSWFRLGPI
                     NVEPSEIARLATILLLAKYLQLKERDMGKIPTLLIAFGICLLPMLLIVVQPAFGDAFA
                     FTPILLSMLFIAGADMYHIGSVVVFFSVAISVPLYIEYHNITLVDPLVGHLSDLGKTD
                     LLPAVRLLKTDIWKFAAKGKIPVGIEGADKDYLVGLAGNQNLRASLVEAVNSVRSDSG
                     GLLLRLLENDTLLLALGIFFALSALVLLILRLTQGTSLANLRKLYIPFGVIGMSLLSA
                     VAVHTTFSFKYHQIARVTAFINPDRFPRDLAYQTRASRAAIGSGELSGRGIFQGDMTT
                     GERPLVPEAYTDFIFTAWCERTGFLGGTLILLLLLSIPLRGMLVSFESRDRFASLLSA
                     GISFMFFFHILMNVGIAVGLLPVTGLPLSFMSYGGSHLITAMVSLGILLSVARRRFAN
                     "
     gene            19351..20037
                     /locus_tag="PLG78_00230"
     CDS             19351..20037
                     /locus_tag="PLG78_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21560.1"
                     /translation="MSSERYEQLHDLLAGYESSILKLESDLQECQGALPGMETTAAEM
                     TPLLPDRAGFARERSIRLMHRTARLHKLFGEFRKEFQILHRHMDKIRRDARLTLIPLP
                     EERKEVRKADYTLFRLGDMSFCVMDRPVRVEQDVSVSNKIADATLDLFPGRDTISIFA
                     PGKSRLGLLYFGEGKTEQAIWFEEMLEPVIESSHMVQLQPAGVAHPRVRGKFRFRGVD
                     YYVVTLSDTE"
     gene            complement(20041..20472)
                     /locus_tag="PLG78_00235"
     CDS             complement(20041..20472)
                     /locus_tag="PLG78_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNE21561.1"
                     /translation="MASNDLILAMGSGFRGAMDIVRENMQKKDKQSTLALSILIHVER
                     QPGISQGELGRMLRRDPMTMSQAVRALQNSSLITSQADGEDRRIKRLNVTKKGKTFAQ
                     SMNGGESKLINALGKEWGKGRVNQFAKDLAEFNEFLTTYNS"
     gene            20734..>23639
                     /locus_tag="PLG78_00240"
     CDS             20734..>23639
                     /locus_tag="PLG78_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNE21562.1"
                     /translation="MAIVENTSRAQGLYDPGLDKDSCGVGFVVDIQGRKTRRIVDLGL
                     QVIHNLEHRGAVGADPLTGDGAGILLQMPDKFCRRAAKEVGIDLPPEGRYAVGFMYLP
                     QNRTQRKAVETIIEKVTVDEGQEFLGWRDVPVQPEFTGEGARSTLPQFRQCFIAASDD
                     IKDTLEFDRRLYLIRRVIDRRVRAEHKLDRSRYYVASFSSRTIIYKGMLLADQLSRFY
                     VDLQAPDMESALALIHMRFSTNTFPTWDLAHPFRMIAHNGEINALRGNINWMAARQMM
                     MESPLYGKDLKRMLPIIMEGQSDSATFDTVLELLVMAGRSMPHAMMMMVPEAWSKNPD
                     MEESRRAFYEYHATMMEPWDGPAAIAFTDGTQIGATLDRNGLRPARYIVTKDDLVILA
                     SEAGTLPIEASNIARSGKLQPGKMFLIDLNEKRIIEDEEIKRQVMTKQPYGKWVEQNM
                     IRMNELPDPTFVHQPDHLTILERQRVFGYTSEDINATIRPMSVTGEEAVGSMGVDAAL
                     AVLSDRPQPLFRYFKQLFAQVTNPPIDPIREELVMELTAYIGPEGNLLDETPAHAHRL
                     ELEHPVLTNNDLEKIRTIARGHFQAVTLNILFDPEAKSGMRNRLDQVCDEAANAVRSG
                     VNLIILSDRGVGPNVAPIPSLLAVAAVHHFLIRAGLRTKTGIVLESGEPREVSHFALL
                     LGYGANAINPYLAFETLADLIEEGGMPEIKSFKDARKNYIKAIGKGLFKIFSKMGIST
                     LQSYCGAQIFEAVGLDSELINAYFTGTPSRVEGCSAEMIEEEVLRRHAQAYDATFHPG
                     LLDPGGVHYWRKEGEAHLYSPTTIHKLQLATETGDYKVFKEFSKAIDDQSKHRITLRS
                     LFDFDTNAQPVPIEEVEPVSTIVKRFATGAMSFGSISWEAHTTLAIAMNRLGGKSNTG
                     EGGEDSIRYAKLENGDSMRSAIKQVASARFGVTTNYLVNADELQIKMAQGAKPG"
BASE COUNT         5662 a         5745 c         6118 g         6114 t
ORIGIN      
        1 cgatcgtcac atccgatttg cggaagggtt tcaccgccgc tggatgggtt ctaacgaagc
       61 caaactcaaa tggtgtgaac gcgtttcctc tatgaagata aacatgctgt gcccccagca
      121 cggagccatc tacacgggag agaatgtggc gcgatttatt tcctggtttg cggagctacc
      181 ggttggatcc ggaaatgttc cgtaataaac tcgcgcgacg tagtgcgccg cgcgagtctt
      241 gccaaagcga attcctaaag cgaagtgctg tacgatacat tcactgcata cagccaccgc
      301 ggaacttttt gtcgcggagt gtacgtgtaa gatgtattgt tcgtcatggc tcggacgcga
      361 gatgtgatca aactatttac aagttgattc ccgaatccct ttgtcctgga tggatcagtc
      421 acgagtccgt cgcccgcagt ggagccacca tctatgtata gcgctctgga tgttctagga
      481 tctccatcta cctggctatc ataaatgtcg tagctatggc gatatgtagc ttccaggcca
      541 aagcgaaatc cgttccagga aatgcctgcc ccaaatggag aatcagcgag gccgtgaaat
      601 gagtcgcttt gtccactcgt gccaaggctc atatatccaa ggccggctga aacatcagct
      661 ttgagattct ctttgatttc aaattctttc gtatacgcca gtttcatata ttgatctgag
      721 ttcactacat ctgcaaggat tgtaaagctg agctcagtca gaaatggagg cgcgtaagta
      781 acatacattt ccgttttctg ataattggct ccgccgtcgc cgaatggatt ggattttccc
      841 tttgggtccg tcctcgtgga agcattgata ccaaaatcaa aattcccgag ttttgtttcg
      901 ctctgatgtc caaagcttaa atcgatttga tctccgcgct tcagaccatt tgcatctttg
      961 tacattttgt aaacgctcag gctatggatg ggtgtagtgg gccaggtagg gagcatcgtg
     1021 agttgatcta caatgtaagt agacgcaggc aatcgagccg tgttgacaag agggttcacg
     1081 gaatcggtca gaagggtttg ctctcctgtc ggaccgtctt cgagtttctg atccacgtct
     1141 ttgtcgtttc tattcgaaag agcaaaagat cctgccgcgt tgaaataaag accggatcca
     1201 gggactttga ttttcgcaga tggctgaaag aaccaggggt cttcttttga attgtaggcc
     1261 tttcctctct gcacgcttct gttgcgccct ctgtcttctc ctctatccag aaagttcgac
     1321 gtgaacttca gctgcacgtc cacggctcca tccgggatcg catctggtgc cggcgcaggt
     1381 gtagccgggg cgggctgggt tacattgttc cgtttgtctt cctccgttgg attctgcgct
     1441 tcgagcgaaa tgtgaagcca gagaagatca aagccacgct tccacctgcg cgcatgaatt
     1501 ttttgtaaat catggattct ccttttggtc ccgctttgcg ggactctggg ccatatctgg
     1561 gaggtgccga ttccagtcaa acagatattg agactcattc gcaatatcat ttgaccgccc
     1621 agggctggcc gattctctgg tcaaaatcca tccatccagg agcaaacagt gcaaaagcaa
     1681 cttctgatta ttcctcaagc gattctcatc acaatttcgt gtcttaagcc tcagcctgtc
     1741 agcgaggctg cagtggtacg tgattatagc ctccttgtgc acgcttctta tcttcaggct
     1801 cgcaaagacg cgcaggccat gcagaaatca gtcagcgact ttctggctgc tccgggcgat
     1861 gcaggattga agtctgcaag agattcgtgg atcaaggcga gagtctccta cagccagacg
     1921 gaagcgtttc gtttttacgg cgggcccatt gatcgcccta aatctcaggg aaaagaagaa
     1981 ggcccagaag ctcttctcaa ttcatggccg gtgaatgaat cctatattga ttacgtgaag
     2041 ggcaatccag gagcagggat catcaacgat cccaggattc agatcaatgc aaccacgtta
     2101 accggcaaga atcaacagaa agatgaggcg gacgtggcca ctggatttca cgcgattgag
     2161 tttctactct ggggccagga tttcaatccg gcgggtccag gggatagacc tgtgagtgat
     2221 tacactacga agtcgaatca tgagcgtcgc aagcagtatc ttcgcgtaac cacagacatg
     2281 gttgtaacgc agctgcgatc tctggaggag gattgggcgc ccgggaaaga aaacaactat
     2341 gccgctgaat tccaaaaact gaactcgaaa gtcgctctcg gtcatatact gactggactt
     2401 gtgactctgg ccggctttga gcttgcgtcc gagagaacgg ccacggcact gaagagtgga
     2461 gatcaagagg atgaacaatc ttgcttcagc gataacacgc acaatgattt cataaataac
     2521 attcaaggaa tattgaacgt ttatgagggt agggtgggcg actactccgg aataggactc
     2581 gatgcgtgga tcaaatctgc ttctccggaa atggcagatc aaatcttaga agcacttcgc
     2641 tccacgaaag acaaagcaat ttctctcccg gttcccgtag actcaattct cgccagtcca
     2701 aaaaactcag actccagaca gaagcttgaa agtctatcgc atgcgcttgc tcaagatgcg
     2761 aaactgtttc agaaggccgg tattcttctt ggagtagatg ctcgtttgat agccgaataa
     2821 ccatggttat gtgcaaagga tttccgattg tactggtcct tttatctttg agcgaatgcc
     2881 gccgtgccag agatccggga ctacaataca gtgggggcga aaccactgtt tttgtcacat
     2941 cctcgcttgc ctttagttct ccagccgcga atttagacgt ggacaaaatg cccgcgttca
     3001 cgtttggcaa tcgcgtcttc aatacgaact gggtcactgc tccctcctca acaaagaact
     3061 tcgatgggct cggacctttt ttcaatcgag tttcatgttc gagctgtcat ctattggatg
     3121 gccgcggtca acctccggaa acgccgaaag acacgatgga ttcgatgctc gtccgcgtga
     3181 gcttacccgg agaatccgag gtgggaggac ccaggcctca tcctatctac ggagatcaaa
     3241 tcaatgatcg cgccatcctg ggagtgaaac cggaaggtcg cataacggtt cgatatgtgg
     3301 agatcgccgg aaactatccg gatggaaagc actacgatct tcgtagaccc gtatattcat
     3361 tccgattgaa ctacggggac gcgcccggga ttctattttc accgcgagtt gcgcctgcga
     3421 ttcatgggct tggtctcctg gaagcaattc cggaggccgc gattctggcc atcgctcgtc
     3481 tccaggcttc ctccggaatc cagggcaaac ccaattatgt tttcaatgct ctcacaggcg
     3541 gaaaatctct cggacgattt ggctggaagg ccaatgttcc tactttgaga cagcagaatg
     3601 cgggagcagc gttaggcgac attggtctga caagtgagat attccctcgc gaaaatataa
     3661 ccgccatcca aaaggacaac aaggctctgg cgtctgtggc cggtttgggt ggcgattcgg
     3721 tagatttgaa aacagagttt ctggatgatt tgactttcta tacacagacg cttggtgttc
     3781 ctgcgcggcg aaatcataag gatccggaaa tccggaaggg agagcagatc ttttctcaga
     3841 tcggatgctc tggttgtcac gtgcccactt tcgagactgg cgagcatccg gccggaatca
     3901 aagaagtcag tcaccaggtg gttcatcctt acacggatct attgttgcac gatatgggat
     3961 cggatctggc agacggcagg ccggactttc aggccagcgg cagtgaatgg cgcacgccgc
     4021 ctctctgggg cattggactt gtggaggccg tcaatcgcca cactactctt ttgcatgatg
     4081 gacgtgcccg gaatctggaa gaagccattc tctggcacgg cgggcaggca gcccgagcca
     4141 gagaggcttt caagaatatg tcagaaaagg agcggactct ccttcttcga ttcctgaaaa
     4201 gcctgtaatg gaatgcgaaa gggttattgc ccctttcgca acctttctag ctccgccttg
     4261 ttctgatcct gcgtactgag tggagagttt tgtttcattt gattgatttc atcgcgtccc
     4321 tgattcgaaa tcttgtcttt ttcttcgcgg ccgccttgag tcattgcgtc tttgtctgca
     4381 cccagttgtg atttgagttt gtccatttcc tcgcgatcgc gctggatttg ttcgcgaaga
     4441 ttttcgagat cctttttgtg ctggtccttc attagctcct gattgctatc cttttgctgt
     4501 tgcatgattt cttcattgga tttcttttgt tcttcgattg tttgttgaat taggattttc
     4561 tgctcctgga attgctggat cagactctga atttcattgc gaccgttttc agtgatggat
     4621 gccaggtcgc gccccaggtc tgccacttcc ttcttatcag aatctgtctg cgtggaaaca
     4681 gtgatctttc cgtctgattg cacttctgct tttttgttct cttcaacaac ttgcgaatct
     4741 ttgagtgtgt catcggcgac ttcaactttg ccttctttga ccatggtttg actcttgcca
     4801 ttctcttctt tcacaaccca ttctgtcccc cgaacggaag caatcgcagt cggagtgact
     4861 acgttgatac gggaagcagc ggcgagtttc tcacttgcga ggaacaccct tcctttctca
     4921 aggcgcagtt gcagttgttc aaatcctttg ctggcaagaa tgcgcgcaga ttcgatcttc
     4981 agtgttgtgt ccggacccat gcggagtttc atggttggtg aaaattgcag atcgacctgt
     5041 ccgtcccggc cggtttggat gacgtatcca ggcatcagga ttccatcttt ggtatagaga
     5101 gaagaagggg agatggtttt gttcgttcct ttgttgtctg tgactgacac ggatccctgc
     5161 agtgccgtaa tggccgctcc ctgtagatcc acttgatctt ttttgcacgc gactgcggca
     5221 gatagcgtca cgaagatagc aactttcagt aggagagctc gagtattcat tgacgcaggg
     5281 gatcattcgg ccttcgcttt gacgacaaaa aaactcccgg gcgtttgttc aaaatcaatc
     5341 tttgtcagag ataaccgttg gttgaaatca tgaagaaaat acttacattt gttgatgata
     5401 tttatgaaga tctggagctc tggtatccga agcttcgggt cgaagaggct ggatggagtg
     5461 ttgaccttgc gggtccagag attaagaaat attcagggaa gcacggatat cctgctagct
     5521 cgaacatcaa gatttctgac gtgcaggaag attattatca agcgcttttg attccaggcg
     5581 gcttcatgcc ggacaagttg cgccgcgatg cgagagtact ttccatcaca aagaactttt
     5641 ttgattcagg caaactggtc gctttcatct gtcatggtgg ctggattcct atttctgcga
     5701 agattcttaa cggaaagaaa gcgaccggat cccgcggaat caaagacgat ctggaaaatg
     5761 cgggcgctat ctgggtggat gaacctgttg tgcgtgatgg aaatttgatt tcaagccgaa
     5821 cccccgtgga tttgcctgcc tttggacgag ctatcacaga atacctggcg gaccactcat
     5881 gaaagattct tacttaagat tgctagaaaa taacaagatc tgggccgcgg aaaagataaa
     5941 agagaatcca aagtattttc agcatctagc ggaactgcag accccagaat tcctctggat
     6001 tggttgttcg gatagccgcg tacctgccaa tgaaatcacc ggaacagatc cgggagaaat
     6061 ttttgttcat cgaaatgtcg ccaaccttgt cgtgcataca gatttaaaca tgctcagcgt
     6121 actgcaattc agcgtggaag tgttgaaagt gaagcacgtg attgtgtgcg gacactacgg
     6181 atgtggtggt gtgaagtatg cgatggacac tcaggatcac ggacttgtga ataagtggct
     6241 cagaaacatt aaagaaattc acgtgaagta cgatgagcag ctttcgaaat taaccgatgt
     6301 gcgagcacgc gcggatcgtc tggtagaatt gaacgtgatt gaaggcgtgc gcagtctggc
     6361 aaagaccagc atcgtacaga aagcgtggaa gacgcgcagc ctgcaaatcc acggttgggt
     6421 gtacggtctg gagaatgggc tgatcaaaga cctcaacgtg atggtgaacg gaatcgcgga
     6481 ccttgaacct atcttccgtc tcgatgtttg agatttattc ctgcgcgtca aagaatcgcg
     6541 caatctctct accaacccct tccgggtctt ccatcgggaa acagtgagtg ccggagtgtt
     6601 tgatccatct ggatttggta tgagatttca gcaagttctt tacagcacta ttgggtgtga
     6661 catctgactt gtgcgcagta ataatcagaa ggggtttctt gacagtgcga aagactctcc
     6721 actggcccgc atgaaagctt cgaaatattt tcgcctcaat ttttggtcca gacatagacg
     6781 atagccggca ttcgttgctt caaatgcagt ttccaggtag ccctcgaagg acgcaggatg
     6841 ccactgcttg aacaatgggt tcatgcgaaa gcttcgacta acaacgcgaa ggtctttgaa
     6901 ttctcttctt cttttttctg caagggcggc cagaggattt ggaatcaaag aggcaaaccg
     6961 ggaggttaga ggagagaaca ccgttgggtc aagaagcgcg ccggccacaa gccttccttc
     7021 catcgacgga gtcgccagaa gcaaagaagc gccgccgatc gaatggccaa ccgcgaagaa
     7081 tcgttcgtag cccaggtgtt ctgcaaatgc agccgcctgc ctgccaaaga attcccagtt
     7141 gtgaaagtct gttgtcgaat cggagccccc gtgtcccatg aagtttagag catggacgtg
     7201 aaagccgtgg tttgccaggt gatcggtcag aggggcatag gatccagccg aaaatccatt
     7261 cgcatgaaag agcagtaaag ggcgaccgct cccctttttg atgtacttca gccgcattcc
     7321 ctcaaactgg aaatccagca tattggccat gggccagggt cgccagagaa cggtcccgga
     7381 ccagaaaaaa tcgtttcccg gctcaggctc tgctcagcgg gtaccattat tgctaatata
     7441 ttaagcaaag gatccgcatg cccaatcaga taacaaaatc cgaagcattg cgcttcatca
     7501 gcgacattac gcacgccatc aaccagagcg acaatgccga aaacgtatta gagcgtattc
     7561 tttcagcatg tatccacgct acgcatgctg aatctggaag cattatgctt atagatgagg
     7621 atggcgaaaa cctgaccatc catgtttccc aggggttggc ccccgaaagt gctcatttaa
     7681 agttaagaat tggtgagggg atcactggca atgtggctcg gactggcaag gccaggatcg
     7741 tcggggacgc gacctctgag tcggactata tccccgtcaa agaaggtttg atttctgaac
     7801 tcgccgtccc tatggccgtt cgcggcgaag tcattggagt catatctgta gactcaagcg
     7861 gcaaaaacgc attcgatcga gatcacgaac acttcctctc catcatgtcc aatctggcgg
     7921 ccggaatctt tgtaaacatc aaagacaacc aactgttgaa gagtcgcgat cgatttcaca
     7981 ctgtgcttca tgagatttcg cgcattgtat ctgcgtccct tcgtcttgag gatgtgttcc
     8041 gcgaaatcat gacgatcaca gaaaaggcgt tcaagcttca tcgcagtacg cttttgctct
     8101 atgatagaca gcaggatgtt ctggcgattg tcgccagtgt tggtttttca aaagatgaac
     8161 tggtagacgt cacttacgca ccaggcgaag gagtcaccgg ttccgttttc gtgaacaaga
     8221 aaccggtgta tattccaagt gtttctaatg agccaggttt tctgaatcga ctgaagacct
     8281 acggcgaaac gggggacatg ggattcttct gctgtccgat tttctacgga accgaagtgg
     8341 tgggagtttt ctctacgttt acgcgtcagc aagggctcga tgcggagtct atgatcgagt
     8401 ttctggaaat tctcgggagt atgatctccc aatccataac gattcaacgt ctggtcaaag
     8461 aagaaacgcg tgtcatcgca tctgagaaca ttgagctaaa gcgagagctt ggatcgagat
     8521 acaagtttgg aagtctgatc ggcaaggcgt ccagcatgat tcgactgttt gagaaagtcc
     8581 gaatcattgc ggattctaga gcatcggtgc ttctgactgg cgaatccgga actggaaagg
     8641 aactcatcgc atccgcagtt cactacaata gtccgcgaag agaccagcca tttgtaaaaa
     8701 tcaactgcgc ggcgatccct gagaaccttt tggaaagcga gttgttcggc catcggaaag
     8761 gatcttttac cggcgcgatt gcggacaaaa aaggaaaatt cgaaacagca gacggcggca
     8821 caatctttct ggatgagatt ggcgaactgg atctgaatct gcagagcaag ctacttagag
     8881 tgctccagga aagagaaatc gaacctgtgg gaggccgcac tcgccaggta gacattcgaa
     8941 tcatcgcagc cacgaatgct gatctggaag cgggaattgc ggataagaaa tttcgcgctg
     9001 atctctacta tcgtttgaat gtaatcaact tgaagattcc tgcgcttcgc gaacgtaaag
     9061 acgacattct tttgcttgtg aatcacttca tcgagaaata cacgaaggaa aatggcaaat
     9121 ctctaactgg catttcgcac gctgcactca agatgctcga agattacgaa tggccgggga
     9181 atgttcgcca gctggagaac gcaattgagc gtgcagtcgt tctttcgcag aacaagatac
     9241 tggacgtgga tgatttcgcg gacacgactc tacccttttc tgcaccgcct cagaatgctg
     9301 gagttcccgc agttcaggca gtggctgtgt cgtctcctgg cgcgcttgaa cttccggatg
     9361 aagagcagtt tcatcatatg gacggacatg tatacgatag cgtgattcag gaagtggaga
     9421 agcggttgat tctacacgct ctcaagaagt tcaaatatac caagacgcag gcagcgcgct
     9481 acctcggaat caatcgcaac acactggata aaaagatccg tgagttgagt atagattact
     9541 gagtttgtgt aactcaggcg gagagtcgtt tagaaaaatg cgggtcccgt tccggggacc
     9601 cgcggagtta cacgaatgac cttttaatca tcggtctttc gcgcaaatcg cgcttaaaaa
     9661 atattacgac tcttgcaatg caatgttgag ctccagcatt ccgagtctgg agtacaaatg
     9721 cagaccgagt gtagttttgt cctgcacttt gagagtttga gtgcgcatat ctattacgta
     9781 aggcacactc atatccaggt ccttctggct gttgaggaag atgttgagcg cgttgcccgt
     9841 aatcatattt ccaagttcgc caagcacgtc cctgtattct ttttctatag aggaacgatc
     9901 tgtgccaggc attagcttct ccgcaatgcg atatgcactt tccgcattca tagaatagat
     9961 gacttctccg tgaacacttc cggtcacatt caagaggatt gcaatttcgt gagaaggggc
    10021 tggagttttc ttaacgagag ttcggccgcg tatgatctct tcctgaagca gatccctgaa
    10081 aaccaggacc gctccttgaa ggaatggatt cacatattca gccttcactc ccctttttta
    10141 tcggcaggtt ttgtgccgtc cagtgtttag aatgaatcat ccggatgaga gaccgggcct
    10201 gtgtctttga cttctgactt gagtttgaaa cggccacgct ctgattcctt cttcaaaaga
    10261 agctcaaagt acaggcgccc tgcctcattc cttttctctt ccgcgatttt ctgtgccctg
    10321 gtttccagtc cagcgtccgc cacggattga acgcccgaga tttccacata gactgtggct
    10381 tctccgtcct gaattggacc aatttttttt acgccatctc cgccgagcgc ccccagaact
    10441 tccgggccat ccagtttcac ggtctctccg gaaggcagag ccactccctt gagctgggac
    10501 agtttgatgt tcacctgcga ggcacgtttc cccgtgattc gctcaatatc ttccagtttg
    10561 aaatctttgc ccagctgaga caattgctgc tttaggcctg cacttgctag ctggcgcttt
    10621 tcagtgagaa cgcggttttt cacaaattcc ttctggcttt cgtaggattt gcgttgtgcc
    10681 ggttcaaaga tcgattgctc tttctcgtgc tttgctttga tttcttcttc cgttacttga
    10741 atgttcccgc ctgatttttc agtgagatcc agagtcgtga aacgaatgat ccgcgcattc
    10801 atcgagatag aatctgcggc tgcttccgca cgcgctgtag gcaaagcctc tcccatagat
    10861 gaaattactt cgtaggcctg ctgtgccgcg cgcatgcgaa ggcggatgtc catcgggaaa
    10921 cttgcgagct gtttgtggta tatttccttt accgttaggc gatcatccgg agctgcattc
    10981 ttttgcgcct cgtacatttc ctgcgcgcct ttcatgagtt catcttgcat gaatttctca
    11041 gatggagcca cacccagcct gtgagcaagc aaaggcagca cgtagagctg tcggatctgg
    11101 ttggcaatgc agttcttgag aatatattcc gggatctggc cgaaggcgct catgttctgt
    11161 ctgcagttgt cttccgccat caggtagatc cgaggctgga tttcctcccc gtcaaagctc
    11221 ccgactctcc cggcccctga gagaatgtca gtgattgtgc tcaagggagt ttttgaaaaa
    11281 gtaagggcca gcatgactcc gagaatgacg atgatcagca cggagccgaa ttttttaatg
    11341 atatctactg aacccatggg ccatacgcgc atcatggaac caattccgcc acaaaatatt
    11401 cctcaagagt caggccgatt gttgagataa tcaagcaggg catgtatttc atcggcatag
    11461 gtcttattct gctcggcctc attagcctgg ctgtactgtt cttttctgtt tcgactgagc
    11521 gtcatcagga agagccgggc catatcatgc ccgtcgtaac ccgtgcgccg gatttgcgcg
    11581 acgaaagcgt tcgccccgcc cccctggcgc cagttcatcc agaagatgca cccgtttccc
    11641 attctcaagc cgtcgcggaa ccttctgcag aaccgtctcc ttccattctg tcgcacgccg
    11701 ctgtgcgcgc cgtttctcct gtggcgcctg ccgtgagcga ttttcgccct gcggcgaagc
    11761 gaaaagatcc ggggcaactt gctgtgttcg gcactctgtt tttggaccac gcacggaatc
    11821 tttctcttct ttcctcgcgt ggcacagaag acttgccccc gcgcatgttc agcgatttca
    11881 aaagagtggg caaagggagt cttgtttgcg aaggagaggg gttcttcttc aagtccggca
    11941 attcagttca tgcatattcc accggcgacc tggagcagat cgtctttttg agttctggag
    12001 tggcgctggt tccaggctcc acgcaatcgc cgatcgccat tttcttgacg gatgaatcgg
    12061 atcgcgtgaa ggctttcatt cgagagaatg cagcctgaat gagaaactac gagtccaata
    12121 catcgtcccg taagcccctt gccattgcca gtctcacgct cttcctgatc ggcggcgcca
    12181 ttgtggccgt cttctatgga gaccaggtag ttgcgtttgc ctcggggcag attctcggca
    12241 gtgcggacac tcgaattcag aagcggctcg cactctttga gcaggaatac aagaaggctg
    12301 gaagaagccc ggccgaactt ctggatgtag ttgaatccac tcgcaaaatc gttcagttga
    12361 tggaacgcga aaagccggcc agcgcagaag tttactatta ctatagtctt ctcaactatt
    12421 acgagattct ggttcgtgtg cctttgaatg gcccttcctt gcttcgcatg actggcagag
    12481 ggtatcttcc tgatccaccc gaaggaatga atctggctcc ggtctctatc agcgcgctcg
    12541 cccaggaaac cgcatccctc gcacgcaaat ccctggcgat tgatccaaag cccgccttcg
    12601 cttcgcgcgt aagggcagct ctcattcttg gtgagctaat ggcgacgggt cgcacggacc
    12661 cgaacctgct gcagaccact ctggccatta agaaggaaga actgacgccg ctcacccagc
    12721 agactacgga ctggatgcgc tgggcgctat ttgccatgca ggggaagtcc ctcgagctgg
    12781 agcaggaatt tacagaaaga aagatgctcg tggaccccgt ggatggatcc aaaccggacc
    12841 cggattcact cgatgcgaac gaggcgcgcc tgctcctcag ccatgcctgt tttaacgcga
    12901 aagactacat tcgggctcta aattacgcca ggcaggtaaa atacaatccc aatactgaaa
    12961 cgatctggaa ggtggacgcg gcccgccttg aaggcgaaat cttcctggtc cagagtggcc
    13021 cgattgcggc acgtcctttt tttgaggaag ccctgaagct caacggaggg aaggataagt
    13081 tcctcgaaga tcgcatcgcc agtctgtctc ctacccgccc ggtgcgaact caggcgccct
    13141 gatgggcatt ttttgccctc attttaaatt gaaatggcca aaatgaaggc actttgtaaa
    13201 tcagcccaca acagcaatta tcatgatgtt tgatcgagtt ttcagtttat tttccaatga
    13261 catgggcatc gacctgggta cggcaaatac cctggtgtat gtgaaaggac agggtatcgt
    13321 tctttcggaa ccctcggtcg tcgcggtgca gtcttctacg ggtaaggtgc tcgctgtcgg
    13381 aaacgaagca aagagaatgc ttggaaaaac tccaggtgac atcgtcgcca ttcgccccat
    13441 gcgagatggt gtgatcgcgg atttcgaaac cgtggaaaag atgattcgct atttcattca
    13501 gaaagttcac aagcgcacta ctttcgtaaa gccgcgcatg gtgatcggtg ttccatccgg
    13561 aattacagaa gtagaaaagc gcgcggtgcg cgaatctgca gaacaggccg gcgccagaga
    13621 aatctttctg attgaagaag cgctcgctgc agccatcgga gcggacatcc cgattcacga
    13681 accagctgga agcatgattg tagacatcgg tggtggaacc actgagatcg cggtcatttc
    13741 tctcggtggt atggtgattt cggattcgat tcgcattggc ggagacgaat tcgacgaagc
    13801 gattgtccgc tatctgcgca ctcagtacaa tctagtgatt ggagatcgca ccgcggaaga
    13861 tgtgaagatc acaatcggaa acgcattccc ggaaaagaaa acggaagtgc gcgaactcaa
    13921 aggtcgcgat gcgatcagcg gtttgccacg tgtactcgaa attgattcca atgaaattcg
    13981 caaggctcta caggagccaa tcgaacagat tctagaagct gtaaagagca cactggagaa
    14041 aactcctcct gaactcgcag cggacatcgt agagcgcggc atcgtgcttg cgggtggcgg
    14101 ctgtctactc aaaggtcttg ataagtacat cagcaaagaa accggggttc ccgtgtttcg
    14161 cgctcccaat ccgctgatct gcgtggctct cggtaccggc aagtatcttg acgagctcaa
    14221 gaatatcaac cgcggcctga agtaacatgc tgtgggagat actctggaga aataagaact
    14281 ccttcagtct caccttctgt atactttttt caactgtttg catgatctgg cagcgcaacc
    14341 cgctcgccag aactgttggt ttctttggca aagtagcgga tcgagcatct ggtgcgatga
    14401 actcgggtct gtctgctacc ggtcggttct gggtggagat cgataagttt cgcgaactcg
    14461 aaaaacgtta tgcgcaagct gaaaagcaaa tcgaagaata caaactagag aaagacaagt
    14521 ttgacgcact caagaaagaa aacgatactc tcagaaaaac attaggcttc gcaatccccg
    14581 cagaatttcc agaagtccgc gccgaggttc ttggcgttcg actcaattca attagccctc
    14641 gcgtaatcat cggcaaaggc gaagagaccg gcattcgtcc gtttatgcct gtgattgcaa
    14701 gatcgcatga cgctgacaac aatacgattc gatctgtggt tggaattgta gtgtctgcgg
    14761 ataacggaac atccgtcgtg cagcctctca ttcatcctgg atttcagctc ggcgttcgac
    14821 ttcctgattc aggtgcatgg gcaatgcttt ctggaaattc aggaaatcca actcaggctt
    14881 tgctcactgt gatttccgga gacggttctc tagatgggaa aaaaccaatc ggacaggtag
    14941 tggtcacaag cggagatggt ggagtatttc caaaaggaat tccggtgggc acgattgtga
    15001 aagaagggcc gcgccagggc caggctagaa cgttcttcgt gaagcccttc gcacaactca
    15061 tgcagcttga ttcagtcaca gttgtaatca agaagccagc ggcgtgggcc cgtaagaacg
    15121 atgaagacaa actcgaagaa tttttgaaga cagagtttgg tgcgccagag tatccagaga
    15181 cgctcatgcg tcttgattcc ggtcgtgacc gcggaccaaa gcagacacag gaccccggag
    15241 aacagaccac tggcacagac cagcagcccg ccactcaggg gcagcagaat gaacccagaa
    15301 ggattcagaa cgtgaatccg cagacgcgct gatatgtttc tcaatttcgc agtagttgca
    15361 attggtatac ttgtatcttt cttcttgaaa gggatcaatt tcctgagcct ggatctggag
    15421 tccgttggcg gtggaattct ctatcctgat ttccttctga ttctcgtgat cttctttgcc
    15481 cttcgtcgcg gggatttttc tgctctgtgg gttggctttt tttcggggct tctggaagat
    15541 tcaggtctgt tgagtttcgc ggataagacc ggggaatttg ttccgattat tggaacgcat
    15601 gcgctcgtgt attgcctgat cgggtttatc ctgggcaaag ttcacaagct tatggataaa
    15661 gatcgcgtgc cgaccatcat cttgattgtc tttgtaacaa gtgtggtcga acgaatcatt
    15721 gtctggcttc tggttggagt gttggaagat tttaacaaat cgttttcagt gctcggtccg
    15781 agtgtttata ctgcattgtt cgctccaatc tggattgcag gcctgagctg ggtctacaag
    15841 atgcaggaat agatgagtcg ttccgcctca gagtttcgtt tagagcagca atttcagacg
    15901 cgcgtgtata ttttcacggg cttcatgatt ctggttggaa cggttctgac tctgcagatg
    15961 ggaaatttgc agcttctcca cggcgcggaa aacagacgca tggcaaagaa atttgtcagc
    16021 cgtcaagaat tcacagtcgc tcctcgcggg ttgattctag atcgcacgac agatcctgcc
    16081 aatccacttg tgaagaactt gatcaccact gatttcgtgg tgttcccttc tagatttgaa
    16141 aatctggaag aaggcaaagg ctacatacgc gaattctgca gaatcatggg tcgcaattat
    16201 gcgaactatg agcaatacct tacgccggaa gcgtggaaag tactcgtaag aagaaatgaa
    16261 tccattactc tcctgactcg catggacaca gcggagcatg aaaggttagg cgagcttcgc
    16321 atggtaacag agtacggatc tttcgttacc aatcatttgc gttattatac aatgggcccg
    16381 gctgccgcac acgtaactgg atacattggt cttccaagca gatcggaact ggataggggt
    16441 ctggcacttc cctatcagat gctcggcaaa ggtgggattg aagcgcgtta cgactctgat
    16501 cttcgcggaa cggacggcgt tcgcattcgc catcgcttcg tggatgaaga ggagcagctg
    16561 gcagaatccg aacagggaaa cagcctggtt ctgacgatcg atcgtcgtgc gcaggccgcg
    16621 gcttacaatt cactcaagaa ctcaggccgt cgcggcgccg ttgttgccat gcgcccatct
    16681 acaggtgaaa ttctggccct ggtatcctat cctgcttttg atcctaacat tctatccagc
    16741 ggttctgcag accagcgctc gcggcatacg aagacggttc aggattatga aggtttctta
    16801 aatcttgcga ttcaggcaaa gtttccgccc gggtctacgt tcaaaccgtt agtcgctatt
    16861 gctgcacttg aaaactccgc tgagaatcat gttacggaag atacttcttt tcattgtgcg
    16921 ggaagctggc ttcttccttc ttctgtagct ggagtgccgc ctgctagatt caactgctgg
    16981 aatgcgggtc acggaacgaa caatctgatc ggcgcgattg cgcaatcatg cacagtgtac
    17041 ttctatcagc tgggttataa gattggcccg aacgcgatga ttcattttgc aaaggccacc
    17101 ggtctggata agaagaccgg cgtagacttg cctggagaga ttcagggatt tgttccagac
    17161 cagagatgga aacagatcac ctggtccagt cgctggtatg acggagacac agtgaacctg
    17221 gcaatcgggc agggctttct ggaagttacg ccaatcgaac tggcggttct gtattctgcg
    17281 atggccaatc gcggaaagat ttacagacca tatttgatca aagaagtgcg cgaccctgtg
    17341 agcaatcgtg tcctcaggca ggttcgccct gatttgattc acgaagttcc tatttcgcag
    17401 aagagcctgg atattgttca ggaaggaatg cgagcggtgg tttcgcgcgg aaccgcccag
    17461 catttgaacc ggcctgatta tcctccgatc gccggaaaga ctggaaccgt gcagacgcga
    17521 agcagaatca aagcggccaa ccatgcgtgg tttgcatctt tcgcacccta cggcgcaccg
    17581 atcgaagagc agattgtagt ggttgtgttt gttgaatttg gtatgggtgg atctgcggct
    17641 gcagcaccta tcgccggtga cgtatttcac gcggtatttc ctgattggac gcccacggtt
    17701 catcctcaaa taaaacctga tcgaaaagaa gaaacgccgc ctaacggtaa accgcaatga
    17761 tcaatcgcta caaactggat gtgtatttac tgggcgcggc tgttgtagtt gttctggccg
    17821 gtattttcac tctctatacg caggaatacc agctggcaga gggttctgga cgctggtaca
    17881 aacagctgat cttctttggg ctcggtttga tcatttgcct tggaattcgc agaatgaact
    17941 atcaggtact cgaagcgtat gcggtgcctg cgtatcttgg atctctgttt ttgctcatgc
    18001 tggtgcttgt attcggggcc acaatcaagg gagcaaaatc ctggtttcgt cttgggccaa
    18061 tcaacgtgga accgtctgaa attgcgcgcc tcgccaccat cttgctttta gcaaagtatc
    18121 tgcaattgaa agagagagac atgggaaaaa ttcccactct actcattgcc tttggtattt
    18181 gtcttttgcc aatgcttctg atcgtggtcc agcctgcgtt cggtgatgct ttcgcattta
    18241 cgccgattct actcagcatg ctttttattg cgggtgcgga tatgtaccac attggctctg
    18301 tggtggtgtt tttctcggtg gccatttctg ttcctctcta cattgaatat cataacatta
    18361 ctcttgtgga tcctctcgtg ggacatctgt ctgaccttgg aaagacagat ctattgcccg
    18421 cagtgcgtct tcttaaaacg gatatctgga aatttgcagc aaaaggaaag atccctgttg
    18481 gcattgaagg ggcagacaag gactacctgg ttggtcttgc cggaaatcag aatcttcgcg
    18541 catccctggt agaggctgtg aattctgttc gatctgattc aggcggattg cttcttcgcc
    18601 ttctagagaa cgatacactt cttctggctc ttggaatttt cttcgcactc agtgcgctgg
    18661 ttctactcat tcttcgtctc actcaaggaa cttctctcgc caatttgcgc aaactctaca
    18721 ttccttttgg tgtaattgga atgtccctct tgtctgctgt tgcggttcac accacattct
    18781 ctttcaagta tcatcagatt gctcgagtga ccgcgttcat caacccggat cgatttccgc
    18841 gagacctggc gtatcagact cgcgcatctc gcgcggccat tggatccggc gaattgtctg
    18901 gtcgaggaat ttttcaggga gatatgacta cgggagagcg gccgcttgta cccgaagcgt
    18961 atactgactt catctttact gcctggtgtg aacgtacagg tttcctgggc ggcacactga
    19021 tccttcttct gcttttgagt attccgcttc gggggatgct cgtaagtttc gaatcgcgag
    19081 atcgttttgc ttccctccta tctgcgggca tttcattcat gtttttcttc catatcctga
    19141 tgaacgttgg cattgctgtg gggctcctgc ctgtcacggg gttacccctc agtttcatga
    19201 gctatggtgg ctctcacctc attactgcca tggtttccct tggaattttg ctcagcgtcg
    19261 cgcgtcgtcg ctttgcgaac tgagcattgg atgtccaaag aggcctgttt tgggctggtt
    19321 tggggatcgt cgaatgccga aaataggagt gtgagttcag agcgttacga acagcttcat
    19381 gacctgctgg ctggatatga gtcctccatt ctgaagctgg agtctgattt gcaggaatgc
    19441 cagggcgctc tcccgggcat ggaaacaacg gcagcagaga tgactcctct gttgccggat
    19501 cgcgccggtt tcgcgcgtga gagatcgatt cgcctgatgc atcggacggc gcgtcttcac
    19561 aaactatttg gagaatttcg taaagaattc caaatattgc acagacacat ggacaagatt
    19621 cgtcgggatg cgcgcctgac gttgattcct ttgccagaag aaagaaagga agtgcgcaag
    19681 gcagactaca ctttgtttcg cctgggcgat atgagtttct gtgtcatgga ccgtcctgtt
    19741 cgagtagagc aggatgtttc cgtctcaaat aagattgcgg atgctacatt ggatctcttt
    19801 ccgggcagag atacgatttc gatttttgca cccgggaaaa gcaggctcgg tcttttgtat
    19861 tttggagaag gtaaaactga acaggcgatc tggtttgaag agatgcttga gcccgtgatt
    19921 gaatcctctc acatggtgca gctccagccg gctggcgtag cccatccacg cgtgcgcgga
    19981 aaatttcgtt tccgcggcgt ggactactac gttgtgaccc tctccgatac ggagtagcgt
    20041 ttaactgttg taagtcgtca ggaactcgtt gaactcagcg aggtcttttg caaactgatt
    20101 cacgcgacct ttgccccatt cttttccgag agcgttgatg agcttgcttt caccgccgtt
    20161 catggactgc gcaaaagtct ttcctttctt tgttacattc agacgcttga tgcggcgatc
    20221 ttctccatct gcctggcttg tgatcaagga agaattttgg agagcacgca ctgcctggct
    20281 catggtcatt ggatcacgac gcagcatgcg tccaagttcg ccctgagaaa tgccaggctg
    20341 tcgttcaacg tgaataagaa tagaaagcgc aagagtggac tgtttgtcct ttttctgcat
    20401 attttcgcgc acaatgtcca tcgcgccgcg aaatccggag cccattgcaa gaattaaatc
    20461 gttcgaagcc ataaatccaa gaaaacaaga aagggatcag agtcaatata aatagtctaa
    20521 accactcgcg aaggggaaaa atggtatagg tttctcaaat aaacattttg gagtgtgtgg
    20581 tgaaagatcg cgcaaacatt tttgcgacaa cagaggccgt cgaatgtttg aattttctta
    20641 ttgaacttgc ctgcagaatc cgcgtgactc ggcatcattt ttgctttaag tacaggccga
    20701 cccgcagttt tttggcaatt cggggctcag gatatggcta tagtagaaaa cacatcgcgg
    20761 gcacagggtc tttatgatcc aggcctggac aaagactctt gcggcgttgg cttcgttgtc
    20821 gacattcagg gccgaaaaac gcgtagaatc gtggatcttg ggctccaggt cattcacaat
    20881 ctagagcatc gcggtgccgt gggtgcagat cctctcacag gggacggcgc gggaattctt
    20941 ttacaaatgc cagacaagtt ttgcagacgc gctgcaaagg aagttggaat tgatctgcca
    21001 ccagaagggc gctatgccgt aggcttcatg tacctgcctc aaaatcgtac acagcgaaag
    21061 gctgttgaga cgatcataga gaaagttacg gtggatgaag gccaggaatt tttgggctgg
    21121 agagacgttc ctgttcaacc tgaatttacg ggagaagggg ctcgctctac tcttcctcaa
    21181 tttcgtcagt gtttcattgc agcatcagac gatatcaagg acactcttga gttcgatcgc
    21241 agattgtatc tgattcgacg cgtcatcgat cgtcgtgttc gcgcagaaca caaactggat
    21301 cgcagtcgtt actacgttgc ttctttttca agtcgcacca ttatttacaa gggcatgctt
    21361 ctggcggatc agctctccag gttttacgtg gatctgcagg ctccggatat ggaatctgct
    21421 ctggcgctta tccacatgcg cttttctacg aatacgtttc ctacctggga tcttgctcat
    21481 ccattcagaa tgattgcgca taacggtgag atcaatgcgc ttcgcggaaa catcaactgg
    21541 atggctgccc gtcaaatgat gatggagtca ccgttatacg gcaaagatct caagagaatg
    21601 cttccgatca tcatggaagg gcagagtgat tctgccactt ttgatacggt tcttgaactt
    21661 ctcgtgatgg caggcagaag catgcctcat gcgatgatga tgatggttcc agaagcctgg
    21721 tcaaagaacc cggacatgga agagagtcgt cgcgcattct acgagtatca tgctaccatg
    21781 atggaaccgt gggatggtcc agcggctatc gcatttacag atggaactca aattggtgcg
    21841 actctggaca gaaacgggct gcgtcctgcg cgctacatcg ttacaaaaga tgatcttgtg
    21901 atccttgcgt ctgaggcggg aactctaccg attgaagcgt cgaacatcgc gcgcagtgga
    21961 aaactccagc cgggcaaaat gtttctcatt gatctgaatg agaaaagaat catcgaagat
    22021 gaagaaatca aacgtcaggt catgacaaag caaccttacg gcaaatgggt tgagcagaac
    22081 atgattcgca tgaacgaact gccggatcca acgttcgttc accagccgga ccatcttacc
    22141 attcttgaaa gacagcgcgt gtttggttac acatcagaag atatcaatgc caccattcga
    22201 cctatgtctg tcacgggcga agaggcagtc gggtccatgg gtgtggacgc agctcttgct
    22261 gttctgtctg ataggccgca gcctctgttc cgctatttca aacagctttt cgcacaggtt
    22321 actaaccctc cgatcgatcc gattcgcgaa gagcttgtga tggagctaac ggcatacatt
    22381 ggcccggaag gaaaccttct cgatgaaacg cctgcgcatg cgcatcgtct cgaactcgaa
    22441 caccctgttc tcacgaacaa tgatctagaa aagattcgca cgattgcacg cggtcatttc
    22501 caggcagtga ctctgaacat tctctttgat ccagaagcaa aaagcggcat gcgcaatcgg
    22561 ctggatcaag tgtgcgatga agcagccaat gcggttcgca gcggtgtaaa cctgatcatc
    22621 ctttctgaca ggggagttgg accgaacgtg gctcctatcc ccagtctgct tgcagtggcc
    22681 gcggttcacc atttcttgat tcgcgccggt cttcgcacga agactggcat tgtacttgag
    22741 tcaggggagc cacgagaagt ctctcatttc gcgctgcttc tgggttacgg cgccaacgcg
    22801 atcaaccctt atcttgcttt cgagactctt gcagacttga tcgaagaagg cggaatgcct
    22861 gaaatcaaga gcttcaaaga cgctcgcaag aattacatca aagccattgg caagggattg
    22921 tttaaaatct ttagcaaaat ggggatttct actctgcagt cctattgcgg cgcgcaaatt
    22981 tttgaagcgg tgggtctgga ttcagagctg atcaacgcat acttcacagg cacgccaagt
    23041 cgcgtagagg gttgtagcgc tgaaatgatc gaggaagaag tacttcgtcg gcacgctcag
    23101 gcatatgatg caaccttcca tcctggcttg ttagatcctg gcggagttca ctactggaga
    23161 aaagaagggg aagctcatct atattctccc acgaccatcc acaagctcca gcttgctact
    23221 gaaacaggcg actacaaagt attcaaagag ttttcaaagg caattgacga tcagtccaag
    23281 catcgcataa cactgcgtag cctttttgac tttgacacga acgctcagcc agtgcccatc
    23341 gaagaggtgg agccggtaag tacgatcgtg aagcgatttg caacaggtgc gatgagcttc
    23401 gggtctattt cctgggaagc gcatacaact cttgccattg cgatgaatcg cctgggcgga
    23461 aaaagcaata caggtgaggg tggggaagac tccatccgct acgcgaagct tgaaaatggc
    23521 gatagcatgc gaagcgcaat caagcaagtc gcatctgctc ggtttggcgt tacgacaaat
    23581 tatctggtca atgcagacga actgcagatc aagatggcac agggcgcgaa accaggaga
//
LOCUS       DAOQJL010000003        18472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1400_length_18472_cov_4.293207, whole genome shotgun sequence.
ACCESSION   DAOQJL010000003 DAOQJL010000000
VERSION     DAOQJL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18472
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_18472_cov_4.293207"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLG78_00245"
     CDS             complement(<1..1213)
                     /locus_tag="PLG78_00245"
                     /inference="COORDINATES: protein motif:HMM:NF039926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNE21563.1"
                     /translation="MTLADLLQKFPWPERYRSLGRPLEWFWNFRVAIPRAKLWPLIMD
                     TSRFNRLMELPAMNFEEKDGILYGSSRNAGFHLEWKEIPWSWTSEHTLTNAREYSKGF
                     AHVVRAVYVLEEESAEAVNLTVYFGWIPRNFFGRVLLFFAGSSIKRSYAKALAAIEAS
                     VNAPGATNPFVPPAPTLSPSSLARLNEIRTTLRSSGVDEAISARLVDYVQNGDDLDLY
                     RIRVGALARIWNVDRRALLRGCLHATRAGLLTMSYDIICPHCRGVRQEVSTLGDVPER
                     GNCEVCEIDFTNESEGIVEITFHVHPSIRSVPRVFFCSAEPAQKRHIKLQQYLEPGSK
                     LDAELPLATGRYRLRVKGFSKPGLLMVGPDPEGFVEWTAEDREFRAESGQMSRFSIKN
                     PTEKGQTFTLET"
     gene            complement(1299..1640)
                     /locus_tag="PLG78_00250"
     CDS             complement(1299..1640)
                     /locus_tag="PLG78_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21564.1"
                     /translation="MNAISRKDFLKKGAVAGAVGAAAVALAYCKKKGGEEASAPSAGG
                     CSDVSGLTQAEKDTRAQLKYMDKSDQEGKACNGCALFVPAAAGAACASCNLVKGPISP
                     EGYCTSWVKKG"
     gene            1802..2509
                     /locus_tag="PLG78_00255"
     CDS             1802..2509
                     /locus_tag="PLG78_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNE21565.1"
                     /translation="MEFAILSKQGLYFLLKWSHFIFGIAWIGMLWYFNFVQGPFFAET
                     DANTKTAATQKLVPRALWYFRWGAAFTLLSGLLLIGLNFHLSGATAGEWATGSWLAKI
                     YPGIIMGIVMAANVWFVIWPRQKKVIAAANGEKVENLPVLARRAFLASRTNTMFSIAL
                     LFFMGAGGSKLPGAFNGANVGAWYGVIALIIGLLEINALVATNGITTKPIEKPVGVIH
                     MGLVLAIVLYVILVAML"
     gene            2561..2887
                     /locus_tag="PLG78_00260"
     CDS             2561..2887
                     /locus_tag="PLG78_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE21566.1"
                     /translation="MKNVLFIAVMALLFHCKQSTENLTPEEKFKAEGRSLFVANCIAC
                     HSANPDQDGSVGPSLRASSFDLLKEKLINGKYPDGYKGKRPISGSMPKFAFTDEQIRS
                     IEAFLK"
     gene            2884..3240
                     /locus_tag="PLG78_00265"
     CDS             2884..3240
                     /locus_tag="PLG78_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21567.1"
                     /translation="MKAVFSFLRAMRSPALAAALLMIATCSSSTSANTELAEAPKLTE
                     IEGTIQVKGSASAPITSIDLPGGLSYTLQGKLEKMVRGMYRGKNLKIAGKIITDAKPG
                     KPGVFEVKEIVVILTQ"
     gene            3282..3647
                     /locus_tag="PLG78_00270"
     CDS             3282..3647
                     /locus_tag="PLG78_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21568.1"
                     /translation="MFPHPEETRDPINSVGYHFDDFALQQVVDALPDPVMARITHMCD
                     LKLQPRFLAALGKRIDRMGISLERETEPDRQKDEIVQKAVTKVVNQLMKQGRIVSTGM
                     KYAGKKLTPAGQTPISRPL"
     gene            complement(3644..6133)
                     /locus_tag="PLG78_00275"
     CDS             complement(3644..6133)
                     /locus_tag="PLG78_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21569.1"
                     /translation="MKYPSSNRLLFAATCTLFVLIATVHCGHPNQDSIDLSGEWKRKE
                     ANLTTVDPMVDSSSWESVHVPGSVVKGLPEKFGILWVKHDFTVPAEILRNENALYMGR
                     VAIAEEVYINGVYIGTGGRWPPKGIVNTSDFFSGWNTPRLYHIPGSLLREGKNEIAVR
                     LYYNNEGWINDAVMIGPVQDLAVLHARTSFLLNDLNRILAFILIAVAVYHTLLFVRRR
                     QDLENIFFAFLCVVWAIQSMNFALPPSLPVDYLSKERVVMGIGPFIPLSFALFFRFFF
                     HLNSKFHKIATWALVLFFVPAFFFIVFSFNLQSIRWKYNLVMPVGGVVILYITVCIVQ
                     AWRAGDRNARLISASFLIIVAMTVNDLVIILSSTRGAIFLMGYAFPTLLFSIALVLAN
                     DFVRTRNQVEELNESLEAKVLERTRELQTSHDKIQELKNQQDGDYFLTSLLTKPLNGN
                     FVSGGPLSLEIFSRAKKQFHFRKWDSEIGGDLCAAHRIELNGRQYVCFLNGDAMGKSM
                     QGAGGALVLGTVFKMIITRTQENVSSLNRYPELWLKECLAELQNVFISFDGSMLVSCM
                     LGLLDEENGFVYYILAEHPAPVIYRHSRAEFPVAVEQIRKIGTQGVEGNLRINTLQLE
                     PGDILVVGSDGRDDLEKILPGGERAINEDETLFLRIVEKARADLPTIVEEIYKNGTVI
                     DDLSLMRMGYLEDAAAPTENAMDVIRELKTALAKAREEKDYVRAQEIARNICERFPSD
                     TDALFLASGLIKQAYSSKPEMLAVARSFGERCKLRDPLHLRNLINLADIYRLMGDSAK
                     AQETWKRAETLDPDHATVQKLKTLLGAGRSG"
     gene            complement(6318..6836)
                     /locus_tag="PLG78_00280"
     CDS             complement(6318..6836)
                     /locus_tag="PLG78_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21570.1"
                     /translation="MLIRLSCMRAWRASVFVISFLTCSLSLDALPLRVFKVSEIVRDF
                     GTLRGQEITVEGRLVRAGYMLRSDGSILFETRTCNLMMCGKDECCNACTADVSLVEDG
                     SFGIALSGRFGGREIGCSGNECAMNCNPEIQKHFRITGLLRVEEDHGGPVRLVLEVRS
                     IGPIEKHAKGST"
     gene            complement(6885..8084)
                     /locus_tag="PLG78_00285"
     CDS             complement(6885..8084)
                     /locus_tag="PLG78_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21571.1"
                     /translation="MSERLYFPTFTRHLSILLVVQSLAGSTSAVAFLVSGFAGTSLAP
                     VRILATLPMSAFVIGVAISSSLAAWTMSRAGRKTGHLIGASIGFSGAACACVALTIHS
                     FWGYSASCVMLGVGTAFNNQVRFTAAEGAPPAQKGLVHSWILMCGIFAAILGPAVAAY
                     GRKMDLFGGAAEYAGSYVLLMVMLLASISLLAFLPSLGTTTQTASDSGKISFRHVLSK
                     PVFWVAATSGTTSFAVMTLLMSATPMQMTMIEHFSQESAVFTIQSHIVAMFLPSLFSG
                     FFVRALGLRRLIYCGLLIFALCVPVAYFAGSLHGYWWALVLLGIGWNFLFLAGSTLLS
                     LSFSGPDRFAAQGLNDTMVFGTQAMASLAAGWLLYTVGWQSMVLIPIPFLVLVLALVI
                     GFGRKVI"
     gene            complement(8089..9096)
                     /locus_tag="PLG78_00290"
     CDS             complement(8089..9096)
                     /locus_tag="PLG78_00290"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNE21572.1"
                     /translation="MQFFSPGAHCPVHLEERKVLLNRASIRGICRGWRDKEWWYTGVL
                     DPESQVYVSWYFLRVNVADAFVFSVFDPSRPGPEHLVKQIFYLEKPPESSREGTYLVR
                     DRRGFFVSYTGRGDGPEPGWSFLLKTKSIEAEIRIEPRRPSFTKFDNAVQDNYGLLNF
                     FGASASGKVQAGGHTYELNSAVAYYDHCFGRIPEQTGWHWIAVQGDKFSLDSLINYGA
                     YAQCYTQGWHSIEGVPRPGQWVRLEQAVSFENSVSFDDKDYLSWKKPWRLTSTDLDLN
                     IEILQHKTDLTRVPPILPFLVKLHHTEAFVRVSGKARIDGVWTQTGDMFGVFEQHYGR
                     W"
     gene            9144..12326
                     /locus_tag="PLG78_00295"
     CDS             9144..12326
                     /locus_tag="PLG78_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21573.1"
                     /translation="MTTCTRLAAIPGALVALFLTTQCGAPVEKLGASKGSARISIRDG
                     SIVTLDGEWEAAWNQIIPPDSAHHGSSFFKLPGYWNDVEPLAAGQPSGHRYATFILRT
                     KGDLPPDTAVYIKEARTAYALYCAKNLIASTGRVGSTWEPDMADLTPRLGKLPTDCLR
                     DPEFTLQVSNFDYSLGGPGSPIRIGNHDLLRIAQIRRDALSFALAGILFVMTLYHIVW
                     WLVRIQDLRNIWFSALSFLCMLRIIGTENAIPRLLGPGTFEMQMKVEFLAFYLMIPVS
                     SFFFQSLFPREIHRIFPRIQAVMAVPFIAATIFLPTRIYSEKNWIYLLAAAIMIVYMI
                     YGIALAAIRKRDGAITVLGGASIAGAAGMNDILVAAGVIPGDFLTPVGFFVLIFSQAT
                     VLARVSAKAFATAEHLSQNLREEVRQQTDELRQASEKLLQADKEKTAFFQNISHELRT
                     PLTLIYGPVVDAVRRKEGIATEELGTVLSNARRLLRLVNQLLDLQKIAAGKMELRLRS
                     MNLGEMLGSISEAFQPYANGKRIQIETQIDGDIPRVAADPDMIEKCVYNYLSNAVKFT
                     PAGGRVRICAHRKDNGVEVAVHDSGAGISDENQAGLFTRFGRSEPSLTRDQEGTGLGL
                     SLVRELIALHGGHVSAKSVPGNGASFYFWLPEISEILPEHDSSVSVMADIELADEVAA
                     DDASAHVQASGTTILLVEDNRGMRGYISGILRRSGYTVVETTDGQHGWTAVRSRRPSL
                     IITDLMMPRMSGIDLIANVRADPELKTLPVILLTAKADTRTRQEAHTAGADNYLAKPF
                     NELELLSVVSNLLTLKSTEARLVREIELARKIQNTLLPAKIPELPGLRIACRYKPMER
                     IGGDLYDFCVYPDGSLGILIADVSGHGIAAAMIASMTKLAFALYGPTAESPADLLQQI
                     NSALLGRTGGNFITAQYAMIPANRKTVTVASAGHPAPLFIRDGETRPMLTRGPMLGIL
                     PSIQLMQFTLEIKSGDRLLLYTDGITEVMNAQKQLFEEKRLVDTLTKTANLPLEASML
                     TLIEEASAFAGTYVFPDDLTLVCFEFDAGI"
     gene            complement(12323..13789)
                     /gene="purF"
                     /locus_tag="PLG78_00300"
     CDS             complement(12323..13789)
                     /gene="purF"
                     /locus_tag="PLG78_00300"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNE21574.1"
                     /translation="MPIRSDLRDRFQDDHQIPREECGIYGISGCKEAANFTYLGLYSL
                     QHRGQESAGIVTSDGQRLFRYAGMGQVAQVFTEEKLRDLSGSSAIGHNRYSTTGASFL
                     RNAQPIRVESNLGGFALAHNGNLVNAWSLRSELERSGSIFQTTIDSEVIVHLMARSGK
                     TDFLDALIYALRQVEGAYSLLILTRDALYAVRDPNGFRPLVMGRRQDGSIVFASETCA
                     FDITDTTYERDVHPGEIVCVDGKGITSYHPFEKRAEALCIFEHIYFSRPDSRIFEQSV
                     YDVRKRLGQVLARAHPCDGDVVVPVPDSSTVAAIGFAEESGIPYTMGLIRSHYIGRTF
                     IEPEQRIRDFGAKIKYNVIRSAIEGKRVVVIDDSIMRGTTSRKLIKMFRRNGAKEVHL
                     RISAPPTRFPCYYGIDIPTRGELIAATHSVEEIIRYLNVDSLGYMSLEEAHSAMMDGT
                     RDRKWCDACFSGTYPLKLDQNDNSNQKDLFPEYLVEER"
     gene            13872..14510
                     /gene="pdxH"
                     /locus_tag="PLG78_00305"
     CDS             13872..14510
                     /gene="pdxH"
                     /locus_tag="PLG78_00305"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNE21575.1"
                     /translation="MPIDPSSLRKEYTAAGLLEESAGFDPFALFDRWFHEAVAAEVRE
                     PTAMTLATSNRQGSPSARIVLMKGYDERGIIFYTNYESHKANDLAENPQAALLFFWAE
                     LERQVRIEGSITKTSRKESDEYFQSRPLESRIGAHASNQSKKIESREVLTQKVREIEA
                     KFGSSVPIPDQWGGYILNPKKMEFWQGRPARLHDRILFSKSDAGWLRERLSP"
     gene            complement(14487..15392)
                     /locus_tag="PLG78_00310"
     CDS             complement(14487..15392)
                     /locus_tag="PLG78_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21576.1"
                     /translation="MDSQVLIVDDDPLIVDLVLQIFADSSHRVFKAANATEAENLVHS
                     ENISLIILDWEMPGRSGIDLLRDLKRDRKYKYLPVIMLTSRSDEADVKAGIGAGAFYY
                     LTKPFDKRILMALVDSATNDFKAIRIREQPDPSVIYSCARSSVFEFRTPEEAVELAKW
                     LARMCPDAQGAQLGLQEILVNAIEHGNLGIAYEEKTQLVQKQALYAEMRRRLLIEPYA
                     SRRARVTFEKRESSYSFFIEDQGEGFDFDMFLSLSPERAFSEHGRGIAIARYLCFDEL
                     DYVAPGNKVHAVIRQKHESRGQSFA"
     gene            complement(15392..17305)
                     /locus_tag="PLG78_00315"
     CDS             complement(15392..17305)
                     /locus_tag="PLG78_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE21577.1"
                     /translation="MPDLTLPTYTNYWIIQKSAEAYPDSIAQMFKEGGSEYRSITFRQ
                     MYDNIKAIAMGLKEAGAAKGDKIGLIADVGQRWIWTSLGVTTIGCVDVPRGTDATEQD
                     LRYIFHHAECKIIFLENAKVFKKVEKFLNEFPGLKTIVFFDDPGSISAPGIQIVTLAS
                     LMENGKKAMGGSDAQFHQIGQSIKEDDLATIIYTSGTTGNPKGVMHTQKSLSWEVDHA
                     IRGIRVPPNGVTMGFLPPWHIAERLIEHVAIRTITAVAFTSVPTLAKDLAVARPTFLL
                     SVPRVWESFYNRVMDNVKKASSFNRGMFMFSSWTAMHFSSYKDILLGLQYRLKKEFIG
                     ITLLRKLGALIALIFLFVPNLIAQALLGRVRQILGGRVDFAISGAGALPEYIDRFFYS
                     IGVPIVETYGMTETVGVSARRKYPGTVIGTVGATFPGVEIKLVDDKGNIVTEPNVKGV
                     AYHRGPHIMLGYYKEPEKTRAVLSEDGWLNSGDILVWTVGGQLKFAGRAKDTIVLLGG
                     ENVEPQPIEDALAQSDLIAQIVVVGQDKKTLGALIVPNAESVKKALMLEGKQAPESIE
                     KWNEDPAIVSMFKNEIKALNSDKAGFKNFEKVTAFTLLSKEFQPGDELTQTLKVKRNV
                     VFDKYQKEIERMY"
     gene            17388..18467
                     /locus_tag="PLG78_00320"
     CDS             17388..18467
                     /locus_tag="PLG78_00320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21578.1"
                     /translation="MRIRKFAVGLWIFIAITAVSGSAYGWSRAAQNESWVWIAFSFIF
                     VCSITGAVVATNSGYRNVNRETDAGARAANAEERARLLIEGSSDLIFTTLPNLQITSA
                     NAAAIAALGDDLNGKNFFDLISFDDISGVGPVLIMGELAELTEKKSKYEKRFRLFGNR
                     GRTPEFVFRMEFLRLPHSEEILIRGTEPGEDPLIHNLDFEELHYSLDSDLFKVETMAS
                     RITRNLAGRLDDELVFGASLGLREMLSNAIEHGSLGITYDEKTVAMTEDRYLALIEQR
                     RKAREYAARKINVRYRLSGEAVEYEIEDQGTGFDHQTQLARRPAATESHGRGIHIARN
                     FFDRVEYNDKGNCVKLRKALASRRI"
BASE COUNT         4849 a         4795 c         4457 g         4371 t
ORIGIN      
        1 tagtttccag agtgaacgtt tgtccttttt ctgttggatt cttgatagaa aagcgagaca
       61 tctgacccga ttccgcgcga aattccctgt cttctgcggt ccattccaca aatccttccg
      121 gatccggccc caccatcaaa agaccgggct ttgaaaaacc tttcacgcga aggcgataac
      181 gaccagtggc cagaggcaat tcagcgtcca gtttggatcc tggttccaga tactgctgca
      241 atttgatatg acgtttctgc gccggctctg cactgcaaaa aaatacacgt gggacactgc
      301 gaatagaagg atgaacatga aacgtgatct ccactatacc ttcagactca ttcgtaaagt
      361 cgatctcgca cacttcacaa tttccgcgct ctggaacgtc gcctaacgtg gatacttctt
      421 gtctaactcc tctgcaatgt gggcagatga tgtcatagct catggtcaat agcccggccc
      481 tcgttgcatg aagacagcct cgaagaagcg cgcgtcgatc cacattccag atgcgcgcca
      541 gagctccgac tcgaattcta tagagatcaa ggtcgtctcc attctgaacg taatcaacca
      601 atctggcaga gatggcttca tccactccgc tggatcgaag cgtggtgcga atttcattca
      661 atctggccaa agaagaaggc gatagagttg gcgcgggtgg cacaaaggga tttgtggcgc
      721 ctggagcatt gacagaagcc tcaatagcgg cgagcgcctt cgcataactt ctcttaatcg
      781 atgaccctgc aaagaaaaga agtactcttc caaagaagtt tcttggaatc cagccaaagt
      841 acactgttaa attcacagct tccgcggatt cttcctccag aacgtacacg gcgcggacta
      901 catgagcaaa accttttgaa tattcgcgag cgtttgtgag agtgtgttca gaagtccagg
      961 accagggaat ctctttccat tcgagatgga agcctgcgtt gcgagaagat ccatagagaa
     1021 tgccatcctt ctcttcaaag ttcatggctg gaagctccat caaacgattg aagcggcttg
     1081 tatccatgat cagtggccat agctttgcgc gcggaatggc aacgcgaaag ttccagaacc
     1141 attcgagggg acgacccaga gagcgatatc gttcaggcca gggaaatttt tgaagtaagt
     1201 cggccagagt catagactcg aaaaaatcaa atgccgacgc aatggcgaat atttttggaa
     1261 ggagaggctg cgcatacgaa tgcgcagccg taagaaactc agcccttttt cacccaggaa
     1321 gtgcagtagc cttccgggct gattggacct tttaccagat tgcaggacgc acacgctgcg
     1381 ccagctgctg ctggaacgaa caatgcacaa ccgttgcagg ctttgccttc ctggtcgctc
     1441 ttgtccatgt acttcaactg tgctcttgtg tctttctctg cctgtgtgag gccggatacg
     1501 tctgagcaac cgcccgctga tggagcagat gcttcttcgc cgcctttttt cttgcaataa
     1561 gccagtgcaa cagccgcagc acccacagcg ccggcaactg cgcctttctt taaaaagtct
     1621 tttctagaga tcgcgttcat ggatcctccg tttaagctac cagaacggct tccctggaag
     1681 gcctaacgaa tcttttttgc ctgactgctc cgaatctggc gcgatttgat actcggtctc
     1741 aaaattttcc tttaactgtg atgagaatgc cagtcactgg cagaaaatct cggaggaatc
     1801 aatggaattc gcaattctga gcaaacaggg actctatttc ctgctcaaat ggagccattt
     1861 tatctttggc atcgcctgga ttggcatgct gtggtatttc aatttcgtac agggtccttt
     1921 ctttgctgaa acggacgcaa acaccaagac agctgctacc cagaaactgg taccaagagc
     1981 gctgtggtac tttcgttggg gcgcggcctt cactcttctc agcggccttc ttttgatcgg
     2041 cctgaacttc catctcagcg gcgcgactgc tggcgaatgg gccacaggta gctggcttgc
     2101 aaagatttat ccaggcatca tcatggggat cgtcatggca gccaacgtgt ggtttgtaat
     2161 ctggcctcgc cagaaaaaag tcatcgctgc tgcgaatggt gagaaagtag aaaatcttcc
     2221 tgtgctcgct cgcagagctt ttctcgcgtc cagaaccaac actatgttct cgattgcact
     2281 gctcttcttc atgggagccg gcggatcgaa acttcctggt gcattcaacg gagcaaacgt
     2341 tggcgcatgg tatggagtga tcgcactcat cattggcctg cttgaaatca atgctctcgt
     2401 tgcaaccaac ggtatcacca caaaaccaat tgaaaaacct gtgggagtaa tccacatggg
     2461 acttgttctt gcaattgttc tctacgtaat tctcgtagca atgctgtaag acttcagggg
     2521 gcggcaaacg cccccatctc acaaactgga atccaaattt atgaagaatg tgttgttcat
     2581 cgccgtgatg gcactcttgt ttcactgcaa gcagagcact gaaaacctca cgccagaaga
     2641 aaaattcaaa gcagaaggcc gcagtctatt tgttgccaac tgcatcgcat gccacagcgc
     2701 aaatccggat caagacggaa gcgtcggccc gtcgcttcgc gcgtcgagtt ttgacttgct
     2761 caaagaaaag ctaatcaacg gtaagtatcc ggacggatac aaaggcaaac gcccgatcag
     2821 tggttctatg cccaaattcg cattcacgga tgaacaaatc cgcagcatcg aggcatttct
     2881 caaatgaaag cggtcttttc ttttcttcgc gcgatgcgaa gcccggctct cgcggctgca
     2941 cttctaatga tcgccacatg ctcttcttcg acatctgcaa acacggaact tgcggaagct
     3001 cctaagctca ctgaaataga aggaaccatt caggtgaagg gttctgccag cgctcccatc
     3061 acttccattg atttgcccgg tggtttgtcc tacactctgc agggaaaact ggaaaaaatg
     3121 gttcggggca tgtatcgcgg caagaacctg aagattgcag gaaagattat tacagacgcc
     3181 aaaccaggaa aacctggagt ctttgaagta aaagagattg ttgtgatcct cacgcagtga
     3241 ctcaatcccg aacgcattca gaccgaaggt aaatcataca tatgttccca catccggaag
     3301 aaacgcgcga cccaatcaat tcagttggtt atcattttga cgatttcgca cttcaacaag
     3361 tggtagatgc tcttccagac ccggtcatgg cgcgcatcac tcatatgtgc gatctgaaac
     3421 ttcagcccag attcctcgca gctcttggaa aaagaatcga tcgcatggga atttcgctcg
     3481 aacgcgaaac ggaaccggat agacaaaaag acgaaattgt gcagaaagca gtcaccaaag
     3541 tggtgaacca gttgatgaaa cagggtagaa tcgtttctac gggaatgaaa tacgctggaa
     3601 agaaactgac gccggcgggt cagactccga tcagtcggcc gctctaaccg gatcgtccgg
     3661 cacccagaag agtcttcaat ttctgtacgg tcgcatgatc gggatcgagt gtttctgccc
     3721 gcttccacgt ttcctgtgct tttgcggaat ctcccatgag cctgtaaata tccgcgaggt
     3781 tgatcaagtt gcgcaaatgc aatggatcac gcagtttgca tctttctcca aaagatcgcg
     3841 ccacagcaag catctcgggt ttagaggagt aagcctgttt gatcaagcca gatgcgagaa
     3901 acaaagcatc tgtgtctgac ggaaagcgtt cgcatatatt ccgtgcaatc tcttgcgcgc
     3961 gaacgtaatc cttctcctct cttgccttcg cgagcgctgt cttcaattca cgaatcacat
     4021 ccattgcatt ttcagtaggg gcggccgcat cttccagata gcccatgcgc atgagggaaa
     4081 gatcgtcaat gactgttccg ttcttataga tttcttccac aatggtgggc aggtccgctc
     4141 tcgctttctc cacaattcgc agaaacagag tttcatcttc gttgatggca cgctctcctc
     4201 caggaaggat tttctcgaga tcatcgcggc catcagatcc aaccaccagg atatcccccg
     4261 gctccaattg caaagtattg atcctgagat ttccttccac tccctgcgtg ccgatcttgc
     4321 gaatctgctc caccgcgaca gggaattctg cacgcgaatg acgatagatc accggagcgg
     4381 gatgctccgc caggatatag tacacaaaac cgttttcctc atccagaagt cccagcatgc
     4441 aagatacaag catgcttcca tcaaaggaaa taaaaacatt ctgaagttca gcaagacatt
     4501 ccttgagcca caattccgga taacggttca aagaactcac gttctcctgc gttctggtga
     4561 tgatcatttt gaaaactgta cccaggacca gcgccccacc tgctccctgc atggattttc
     4621 ccatggcatc tccattgagg aagcaaacat attgcctacc gttcaattcg attctatgcg
     4681 cagcacaaag gtctccgccg atctcagaat cccacttgcg aaagtgaaac tgcttcttcg
     4741 cacgagagaa gatttccagg ctcagaggac cgccggacac gaagttgccg ttcaaaggtt
     4801 ttgtcaatag cgaagtaagg aagtagtcgc catcctgctg atttttcagt tcctgaattt
     4861 tgtcgtgact ggtttgcagt tctcgcgtac gttctagcac ttttgcctca agcgattcat
     4921 ttaattcttc tacttgattt ctcgtacgca caaagtcatt ggccagaacc agagcaatgc
     4981 tgaagaggag tgttgggaaa gcgtatccca tgagaaaaat cgcgccgcgt gttgatgaga
     5041 gaatgatgac aagatcattc actgtcatag caacaatgat gagaaaagaa gcggagatca
     5101 gtcttgcatt tctatctccc gcacgccacg cttgcacgat gcaaacagtg atgtatagaa
     5161 tcacaacacc acccacaggc atcacaagat tgtacttcca acgaatcgac tgcagattga
     5221 aggagaatac tataaagaag aaggcgggga caaagaacaa cacgagggcc cacgtcgcaa
     5281 tcttgtgaaa tttcgaattc agatgaaaaa agaaccgaaa gaaaagcgcg aagctcagag
     5341 gaatgaaagg cccgatcccc ataaccactc tctctttgct cagatagtcc acgggcaaag
     5401 aaggaggaag agcaaagttc atactctgga tggcccagac cacgcataag aatgcgaaga
     5461 agatgttctc cagatcctga cgccttcgca caaaaagcaa ggtgtggtaa accgcaactg
     5521 caatcaggat gaatgcgagg atgcgattga gatcattgag cagaaaactt gtgcgagcat
     5581 gcaagaccgc cagatcctgc accggaccga tcatgactgc gtcattgatc catccctcgt
     5641 tgttgtagta gagtctaacg gcgatttcat tttttccttc gcgcaaaaga gatccaggaa
     5701 tgtgatagag cctgggagtg ttccatccag aaaagaaatc ggaagtattg actattccct
     5761 tgggcggcca ccttcctccc gttccaatgt agactccgtt gatgtatact tcttccgcaa
     5821 tcgccactcg gcccatgtag agcgcattct cattgcgaag aatctctgcc ggcacagtga
     5881 aatcatgttt cacccataga attccaaatt tctccggcag ccctttgacg accgatccag
     5941 gcacatgcac agactcccag gaggaagaat ccaccatggg gtcgactgtt gttagattcg
     6001 cttcctttct tttccattct ccggaaagat caatggaatc ctggtttgga tgtccgcagt
     6061 gaacggtggc aatcagcaca aatagcgtac aagtcgcggc aaaaagaagc ctgttgctcg
     6121 aagggtattt cacaggcctg caggatttaa gatcgagact ttcaggcaat cacaaatagc
     6181 gattcgcggg gcttctcaaa taagattgta gccttatttt tttgcgcccc cttccccgat
     6241 gaagggaatt ttggccgcgc gaaattcctt ccaatgacgc tcgccgcatc gtatttcgaa
     6301 aaagcatacc acaggggtca ggtggaacct ttcgcatgtt tctctatggg tccaatggac
     6361 cgcacttcaa gaaccagccg caccggacca ccgtgatcct cttcgacacg cagtagaccc
     6421 gttatgcgga agtgtttctg tatctctgga ttacaattca ttgcgcattc atttccggaa
     6481 catccgatct ctcttccgcc aaatcttcct gagagagcta tcccaaagga tccatcttcc
     6541 accagagaca catccgcagt gcatgcattg caacattcat cttttccgca catcatcaaa
     6601 ttgcatgtgc gtgtttcaaa aagaatcgat ccgtctgatc gaagcatata gccggctcga
     6661 acaaggcgcc cttctactgt gatctcctga ccccgaagag taccaaagtc ccgaacgatt
     6721 tcgctcacct tgaaaaccct gagcggcaac gcgtctagag aaagactgca ggtgagaaac
     6781 gagattacaa agacggaagc tctccaggca cgcatgcagg atagacgaat gagcacgtgc
     6841 tagcgttgtt ttttattgtg ctcttagata aacgatcacg ccctctaaat cacttttctt
     6901 ccgaacccga tgacgagtgc cagaaccagg acgagaaatg gaataggaat caggaccatg
     6961 gattgccaac caaccgtata caatagccac ccggccgcaa gactcgccat agcttgcgtg
     7021 ccaaacacca ttgtatcatt taagccctgt gcggcaaaac gatccgggcc tgaaaaacta
     7081 agagagagta aagtagatcc cgccagaaac agaaagttcc agccaatccc cagaagcacg
     7141 agcgcccacc agtagccatg caaagaaccg gcgaagtacg cgacggggac gcacagagcg
     7201 aagatcaaga gaccgcaata gatgagacga cgaagtccca gagctctcac aaaaaatccg
     7261 ctaaacaaag aaggaagaaa catggcgacg atgtggctct gaatggtaaa cacagcactc
     7321 tcttgactga aatgctctat catcgtcatc tgcatgggtg tggcagacat aagaagagtc
     7381 atcactgcga aggacgtcgt gccggatgtg gcggcaaccc agaacactgg ctttgacaga
     7441 acatgcctga agctgatctt cccggaatct gatgccgttt gcgtggttgt tccgagagac
     7501 ggaagaaatg cgagaaggct gatcgatgcc aaaagcataa ccataagtaa tacgtatgaa
     7561 cctgcatact ctgccgcgcc tccgaagaga tccatctttc tgccatacgc tgccacagcc
     7621 ggcccaagaa tggctgcaaa gatcccgcac atcagaatcc aggaatgcac aagacccttc
     7681 tgcgctggag gcgcgccttc cgccgcggta aacctgactt gattgttgaa ggctgttccc
     7741 acgccgagca tcacgcacga ggcagaatac ccccagaacg aatgaatcgt cagtgcgacg
     7801 caagcgcagg ctgctccaga aaatccaatg ctcgcgccaa tgagatgccc ggtttttcta
     7861 ccagccctgg acatggtcca ggcagccaga gaactggaga tcgccacccc aatgacaaac
     7921 gcggacatgg gcagagtagc caggattctt acaggagcta gactggttcc tgcaaatccg
     7981 ctcacgagaa acgcaaccgc agaagtagag ccggccagag attgaactac aagcaggatg
     8041 ctcaggtgcc ttgtgaaagt gggaaagtac agtcgttcgc tcaaggcatc accatcttcc
     8101 gtagtgctgc tcaaagacgc caaacatgtc tccggtctgt gtccacactc cgtctattct
     8161 agctttgccc gaaactcgaa cgaaagcctc cgtgtgatgg agtttcacca aaaacggaag
     8221 tatgggcgga acgcgcgtaa ggtcagtctt gtgctgcaat atttcaatat ttaaatccag
     8281 gtccgtggac gttaggcgcc atggcttctt ccaggacaga tagtctttat catcaaaaga
     8341 aacactgttt tcaaaagaga ctgcctgctc cagacgcacc cactggccag ggcgtggaac
     8401 accttcgatc gaatgccatc cctgcgtata acactgcgca tacgctccgt agttaatgag
     8461 cgaatccaga gaaaacttat ctccctgaac agcgatccag tgccacccgg tctgttcggg
     8521 aatccggcca aagcaatgat catagtacgc aacagcagaa ttcaattcat atgtgtgtcc
     8581 gccagcttgc acttttccgg atgcactggc tccaaaaaaa ttcaacagac catagttgtc
     8641 ctggacggcg ttgtcgaatt tggtgaatga aggtcttcga ggttcaatgc gaatctccgc
     8701 ctcaatcgat tttgttttca gtaagaacga ccagccgggt tctggaccgt ctccgcggcc
     8761 agtgtaagat acaaaaaaac cacgcctgtc gcgaaccagg tatgttcctt ctctagacga
     8821 ttcaggtggt ttttctaaat agaagatctg cttgaccagg tgctctggcc caggccgcga
     8881 aggatcgaaa acggaaaata caaacgcatc cgcgacgttg acgcgcaaaa aataccagct
     8941 gacatacact tgagactctg gatccaaaac ccctgtatac caccactctt tgtcgcgcca
     9001 tcccctgcag attcctctga tgctcgcacg gttcagaagg acctttctct cttccagatg
     9061 cacggggcag tgcgccccgg gagagaagaa ctgcaatccc aaaaaatgct gggttcgcca
     9121 gcgcgctgtt tcaatatccg tcaatgacga cgtgcaccag gctcgcggcg attccaggtg
     9181 cgctggtagc attgtttctc acaactcaat gcggcgcacc ggtggaaaag ctaggggcaa
     9241 gcaaagggtc agcaagaatt tccatccggg atggaagcat tgtaactctg gacggagaat
     9301 gggaagcagc ctggaatcag atcattcctc cagacagtgc gcaccacgga tcttcgtttt
     9361 tcaagctacc tggatactgg aatgacgtgg aacctcttgc ggcggggcaa ccttccggcc
     9421 accgttatgc gacattcatt ctgcgaacaa aaggagatct gccgccggac accgcagttt
     9481 acataaagga agcaagaact gcgtatgcac tttactgcgc aaagaatcta atcgcaagta
     9541 ccggaagagt tgggtccacg tgggagccgg atatggcaga tctcactcct agactgggta
     9601 aactcccaac agactgcctc agagaccctg aattcacact ccaggtgagc aacttcgact
     9661 attccctcgg gggcccgggt tctcctattc gcatcgggaa tcatgacctg cttcgcatag
     9721 cacagattag acgagacgcg ctgagtttcg cactggctgg catcctgttt gtcatgacgc
     9781 tctaccacat tgtatggtgg cttgtgcgta ttcaagacct gcgcaacatc tggttttcgg
     9841 cactttcctt tctttgcatg ctgcgcatca tcgggacaga aaacgcaatt cctcgccttc
     9901 tcgggcccgg cacatttgag atgcaaatga aggtggaatt cctggccttc tatctaatga
     9961 ttcccgtttc gtcatttttc tttcaatccc tgttcccgcg tgaaattcac aggatcttcc
    10021 cgcgaatcca ggccgtaatg gcggtgcctt tcatcgcagc cacgatcttc cttcccactc
    10081 gcatttactc tgagaaaaac tggatctatc tattggccgc tgcaatcatg atcgtttaca
    10141 tgatctacgg cattgctctg gccgccattc gcaagagaga tggagcgatc acagttctcg
    10201 gcggtgcaag cattgccggt gcagccggga tgaatgacat acttgtggcc gccggcgtca
    10261 ttcccggaga tttcctcacc ccggttggat tttttgtttt gattttttct caggccactg
    10321 tccttgcacg cgtttccgca aaagcatttg caactgcgga acatctgtcc caaaatttgc
    10381 gcgaagaagt gcgacagcaa acggatgaac tcagacaggc gtccgaaaaa ctacttcagg
    10441 ctgacaagga aaagactgct tttttccaga acatatcgca cgagctccgc actcctctca
    10501 ctttgattta cggtccggtc gtggatgcag tcagaagaaa agagggaatc gcaacagaag
    10561 agctgggcac agttctttcc aatgcgcgaa gacttcttcg tctcgtaaat caactcctgg
    10621 atttgcagaa aatcgccgcg ggtaaaatgg aactgcgact acgatccatg aacctgggcg
    10681 aaatgctcgg atccatcagc gaagcattcc agccttatgc aaacgggaaa cgaatccaga
    10741 tcgaaacgca aatagatggg gacattccac gagtggcagc agacccggac atgatcgaaa
    10801 agtgcgtgta caactatctg tcgaatgctg tgaagttcac acccgccggc ggtagggttc
    10861 gcatttgcgc gcatagaaaa gacaatggtg tggaagtggc agtgcatgat agtggagctg
    10921 gaatttcaga cgaaaaccag gccgggctct ttacacgatt cggaagaagt gaaccgtcgc
    10981 ttacgcgcga tcaagaagga actggacttg gcctttccct ggtaagagaa ctgatcgcgc
    11041 tgcacggcgg ccatgtatca gctaagagtg ttcccggcaa cggagcctct ttctatttct
    11101 ggcttcctga gatttccgaa attctaccgg aacacgattc aagcgtttcc gtcatggcgg
    11161 acattgaact tgcagatgag gtggcagcgg atgatgcgag cgcacatgta caggcttcgg
    11221 gaactaccat ccttcttgta gaagacaata gaggaatgcg tggatacatc agcggaattc
    11281 tacgaagaag cggatacaca gtagtagaaa caacggatgg ccagcacgga tggactgcag
    11341 taagatccag acgcccttcc ctcatcatca cagatctcat gatgccgcgc atgtccggaa
    11401 tcgacctgat tgcaaacgtg cgggcggacc ctgaactaaa aactctaccg gtaatattgc
    11461 tcaccgcgaa agcggatacg cgaaccaggc aggaagctca cacggcaggc gcagacaatt
    11521 atctcgcaaa accgttcaat gagctggagc ttctgtctgt agtgtccaat ctgctcactc
    11581 tcaaatccac agaggcaaga cttgtgcgcg aaatcgaact ggcgcgcaag attcaaaaca
    11641 ctttattgcc tgcgaaaatt cctgaactcc cgggccttcg cattgcatgc agatacaagc
    11701 ccatggaaag aatcggcgga gatttgtatg acttctgcgt atatccggat ggaagcctgg
    11761 gaattttaat cgctgatgtt tcaggccatg gaattgccgc ggcgatgatt gcatccatga
    11821 ctaaacttgc cttcgcgctc tacggaccaa ccgcagaaag cccggcggac ttactccaac
    11881 aaatcaatag cgcactcctg ggcagaacgg gaggcaattt catcaccgct caatacgcga
    11941 tgataccggc gaacagaaaa actgtaaccg tggcttctgc agggcatcca gctcctctat
    12001 tcattcgcga cggcgaaacc agacccatgc tgacgcgcgg acctatgctc ggaattttac
    12061 cgtccattca attgatgcag tttactctag aaatcaaatc gggcgacaga cttcttcttt
    12121 atacagatgg aatcacagaa gtaatgaatg ctcaaaagca actgttcgaa gaaaaaagac
    12181 tggtagatac tctcacgaaa acggcaaatc ttccactcga agcgtccatg ctcactctca
    12241 tcgaagaagc aagcgcattt gcgggcacat atgtctttcc agatgatctc actctggttt
    12301 gttttgaatt tgacgcgggg atttagcgtt cttctaccag gtactctgga aacaaatctt
    12361 tctgattgga attatcgttt tgatccagct tgagcgggta tgtacctgag aaacacgcat
    12421 cgcaccattt gcgatcacgg gtgccgtcca tcatcgcaga atgagcttct tccaggctca
    12481 tgtatcccag tgaatctaca ttcaaatatc gaatgatctc ttccacagaa tgcgtggctg
    12541 cgatgagctc tcctcttgta ggaatatcaa tgccatagta acagggaaac cgggtaggtg
    12601 gagcgctaat tcgaagatgg acttcttttg cgccatttct tctgaacatt ttgatgagct
    12661 tgcgcgatgt ggttccgcgc atgatcgaat cgtcaatcac cacaacgcgt tttccttcaa
    12721 tcgcagaacg aatcacattg tacttgatct tcgcaccaaa gtcgcggatg cgctgttcgg
    12781 gctcaatgaa ggtgcgacca atgtaatgag atcgaatgag tcccatggtg tatggaattc
    12841 cggattcttc cgcaaaacca atggcagcta ccgtcgacga atcaggaaca gggaccacca
    12901 catctccatc gcaaggatga gcgcgtgcaa ggacctggcc aagacgcttt ctcacatcat
    12961 agactgactg ctcaaaaatg cgagaatcgg gacgagaaaa atagatgtgc tcgaatatgc
    13021 agagtgcttc cgcacgtttt tcaaaaggat gataactggt aattcccttc ccatccacgc
    13081 atacgatttc acccggatgg acgtcgcgct catacgtagt gtctgtaata tcaaaggcgc
    13141 acgtttcaga tgcaaaaacg atcgaaccgt cctgccttct tcccatgacg agaggacgaa
    13201 atccgtttgg atcgcgcacc gcatagagcg cgtcgcgtgt caaaatcaag agactgtagg
    13261 caccttccac ctggcgaagt gcatagatga gtgcgtccag gaaatcagtc ttcccactgc
    13321 gcgccatgag gtgaacaatc acttctgagt cgatcgtagt ctgaaagatc gatcctgatc
    13381 tttcaagctc actgcgaagc gaccaggcat tgaccaggtt tccgttgtga gcgagagcaa
    13441 atccacccag attggattct acgcgaatgg gttgcgcatt tcttagaaaa gacgcgcccg
    13501 tggttgaata gcggttgtga ccgatggcgg aagaacccga tagatctctg agtttttctt
    13561 ccgtaaatac ctgtgccacc tggcccatgc cagcgtagcg aaacagtctc tggccgtctg
    13621 aggttacgat gcctgcactt tcctggcccc ggtgctgcag agagtacaga ccgagatagg
    13681 tgaagttggc tgcttccttg cagcctgaga ttccatagat cccgcactct tcgcgcggta
    13741 tctgatggtc gtcctggaac cgatcacgca gatcggagcg gatgggcata cagctacgtt
    13801 ttcacaaggg gctacggggg aaatgttttt ttttctgatt gaacgcttga aagtgcacgt
    13861 tttgttgaac catgcccatt gacccttcca gcctccgcaa agagtacaca gcggccggtt
    13921 tactcgaaga atccgctgga tttgatccgt ttgctctctt tgaccgatgg ttccacgagg
    13981 ctgtggcggc ggaagttcgc gagcccacgg ctatgacgtt ggccacctca aatcgccagg
    14041 gcagtccctc tgcgcgcatc gtcctcatga aagggtatga cgaaagaggg attatcttct
    14101 atacaaacta cgaatcacac aaggcaaacg atctggcaga aaacccgcag gctgcacttc
    14161 tgttcttctg ggctgagctg gaaagacagg tccgaatcga aggaagcatc acaaagacaa
    14221 gcagaaagga gtcggacgag tatttccaga gtagacctct ggaaagcaga atcggagcgc
    14281 atgcatccaa tcaaagcaag aagattgaaa gtcgcgaagt acttacgcaa aaggtacgcg
    14341 aaatagaagc gaagtttggt tccagtgttc ctatcccgga tcaatggggt ggatacatac
    14401 tcaatccgaa aaaaatggag ttctggcaag gcagacccgc tcgcctgcac gatcgaattc
    14461 tctttagcaa gagtgacgcg ggttggttac gcgaaagact gtccccttga ttcgtgcttc
    14521 tgtcgaatga ctgcgtgcac cttgttcccg ggcgcaacgt agtccagttc atcaaaacac
    14581 aaataacgcg cgatggcgat ccctctgccg tgctctgaga acgcgcgctc cggtgaaaga
    14641 gaaagaaaca tgtcaaaatc aaagccttca ccttgatctt ctatgaagaa agaataactg
    14701 gactctctct tttcaaaagt aacgcgcgct cgccgactgg cgtacggttc tatcaagagc
    14761 cggcggcgca tctccgcgta cagagcctgc ttctgcacga gttgcgtctt ctcttcgtat
    14821 gcgatgccca ggttgccatg ttcaattgca ttgaccagga tttcctggag gcctaattgc
    14881 gctccctgag catcaggaca catgcgcgca agccacttcg caagctctac cgcttcctcc
    14941 ggagtgcgaa attcaaatac agaagagcgc gcgcaggaat aaattacgga tggatccggt
    15001 tgctctctga tgcgaattgc tttgaagtcg ttagtcgcgc tatctacgag cgccatcagg
    15061 attcgcttgt cgaaaggttt ggtcagataa taaaatgcac ccgcaccaat gcccgccttt
    15121 acatcagctt catctgatct gctggtgagc atgatgaccg ggagatattt gtatttcctg
    15181 tcccgcttca gatcccgcaa taaatcaatg ccagatcttc ccggcatctc ccagtccagg
    15241 atgatgagcg atatgttttc cgaatgcacc aggttctctg cttccgttgc gttggccgcc
    15301 ttgaatacac ggtgacttga atcggcgaag atctgtaaga ctagatccac aatcagcgga
    15361 tcgtcatcta caatgagaac ctgcgagtcc atcaatacat acgttcaatc tctttctgat
    15421 acttgtcaaa tacaacgttt cgcttcactt tcagagtttg cgtcagctca tctcctggct
    15481 gaaactcttt ggagagcaag gtgaatgctg tgaccttctc aaagttcttg aatccggcct
    15541 tgtccgaatt gagggctttg atttcgtttt tgaacatcga tacaatcgcc gggtcttcat
    15601 tccatttctc aatggattcc ggagcctgct tgccttcgag catcagagct tttttcactg
    15661 attctgcatt gggaacgatc aatgctccca gagtcttctt gtcttgaccc acgaccacaa
    15721 tctgagcaat caaatcgctc tgtgccagtg catcttcgat tggttgtggc tccacgtttt
    15781 ctccaccaag gagtacaatc gtatccttgg cgcgaccggc gaacttcagc tgccctccca
    15841 cggtccagac caggatgtca ccggagttca accagccgtc ttctgaaaga actgcgcgcg
    15901 ttttttcagg ctccttgtag tagccaagca tgatgtgcgg accgcgatga taagctacgc
    15961 ctttcacatt tggttccgtt actatatttc ctttatcatc taccagcttg atttctactc
    16021 caggaaatgt agcgcccaca gtaccaatca ccgtgccagg atacttgcga cgcgcgctca
    16081 ccccgaccgt ttccgtcatt ccatacgttt caacaatcgg aacgccaatg gagtagaaaa
    16141 accgatcaat gtattctgga agtgcaccgg cacctgaaat cgcgaaatca actcgaccac
    16201 ccagaatctg tctgactcga ccaagcaaag cctgcgcgat gagattggga acaaataaga
    16261 atatgagcgc aattaatgcg ccaagctttc taagcaatgt gatcccaatg aattctttct
    16321 tcagtctgta ctgtagacca agaagaatgt ctttgtagga agagaagtgc attgcagtcc
    16381 aggacgaaaa catgaacatc ccgcgattga agctgctcgc tttcttcaca ttgtccataa
    16441 cacgattgta gaaactctcc cacacgcgag gcacagacag aaggaatgtt ggacgtgcca
    16501 cagcgagatc cttcgcaaga gtaggaacag aagtaaacgc aactgctgtg atggtgcgaa
    16561 tggccacgtg ttcaatcaag cgctctgcaa tatgccaggg gggaagaaac cccatggtta
    16621 caccgttagg cggcactcga atcccgcgaa tcgcatgatc tacttcccag gaaagactct
    16681 tctgcgtgtg catcacaccc tttggattgc ccgttgtgcc tgacgtgtag atgatggttg
    16741 ccagatcgtc ttccttaatg gactgaccga tctggtgaaa ctgcgcatcg cttccaccca
    16801 tggccttctt tccgttttcc atcaacgacg caagagtcac aatttgtatg cccggtgcac
    16861 tgatggatcc aggatcatca aaaaagacga ttgttttcaa acctggaaat tcgtttagaa
    16921 atttctccac tttcttaaac actttcgcgt tctcgagaaa gatgattttg cattctgcgt
    16981 gatgaaagat gtagcgcaaa tcctgttctg tcgcgtctgt tccacgaggc acatccacgc
    17041 aaccaatggt cgtgacacca agactggtcc agatccatct ctgaccaacg tctgcaatca
    17101 agccgatctt atctcccttc gcggcacctg cctccttgag tcccatggca atggccttaa
    17161 tgttatcata catctggcga aacgtgatgg agcggtattc tgagccaccc tccttaaaca
    17221 tttgcgcgat ggagtctgga taggcttctg cggacttctg gatgatccag tagtttgtat
    17281 aggtgggcag tgtcaaatct ggcatgccat acctgccagg cctcatagcg gaatgtcaat
    17341 tcaatttgtt ctgattgacc caggattcag agcatgcccg attcggcatg cgcattcgta
    17401 aatttgcagt cggcctgtgg atcttcattg caatcactgc cgtttctggt tccgcctatg
    17461 gctggagcag agccgcgcaa aatgagagct gggtctggat tgcgttctca ttcatttttg
    17521 tgtgcagcat cactggagct gttgtggcga ctaacagtgg ttatcgcaac gtcaatcgtg
    17581 aaacggatgc aggtgcccgg gcagcaaatg cagaagaaag agcaagactc ctcatcgaag
    17641 gatcctctga tttgatattc acaacgcttc ccaatttgca gatcacgtcc gcgaatgcag
    17701 cggcaatcgc agcgctgggc gatgatttga acgggaagaa cttctttgat ctcatcagtt
    17761 tcgatgatat ttcaggtgta gggcctgtct tgatcatggg agaactcgct gaactcacag
    17821 aaaagaaatc aaaatacgaa aaacgatttc gcctctttgg caatagaggc cggactccag
    17881 aatttgtatt tagaatggaa ttcttgagac ttcctcattc ggaagaaata ctcatccgag
    17941 gaacagaacc cggagaagat ccgttgattc acaacctcga ctttgaagaa ttgcactact
    18001 ctctagacag cgatctattt aaggtagaga cgatggcaag caggatcact cgaaacctcg
    18061 caggcaggct ggacgacgag ctggtttttg gcgcaagcct tgggctcaga gagatgcttt
    18121 ccaatgcaat cgaacatggc agtctgggaa ttacctacga tgaaaagacg gttgccatga
    18181 cggaagaccg ttatctggca ttgatcgaac agaggcgaaa ggctcgcgag tatgcggcca
    18241 ggaagatcaa cgttcgctac agactgtccg gcgaggcagt ggaatatgaa atcgaagacc
    18301 agggaactgg atttgatcat cagactcagc tggcccgacg tcctgctgcc accgaatcac
    18361 acgggcgcgg cattcacatt gcgcgaaatt tcttcgatcg agtggaatac aacgacaaag
    18421 gaaactgcgt gaaactgaga aaggcgctgg cttcgaggag aatttgagaa ga
//
LOCUS       DAOQJL010000004        17719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1508_length_17719_cov_4.442595, whole genome shotgun sequence.
ACCESSION   DAOQJL010000004 DAOQJL010000000
VERSION     DAOQJL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17719
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_17719_cov_4.442595"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLG78_00325"
     CDS             complement(<1..837)
                     /locus_tag="PLG78_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE21579.1"
                     /translation="MKSLILCAALWFAANLYASPLEEIIDQHPLVQSQSATAGASRHA
                     ARSTMFGYPDPEIEISRSRGREDMIQALPDQMVDRKKITGSEFEVRQPIPFPGKGTVE
                     SLRLGAKADEETLRIRLIKNRMAREFLMLRTRERLIQARIKLNSQTQERLRTLETLAR
                     IRYETGKTPLVDLSMVHVRESEAALAVNQAQSEYASVHRAVEYYTTSSGETHNMSDDQ
                     VDSELAELETRVRNLAAKFEEHSLEMKMAESQIKQGDLDDTRAWMNYLPDFEVFAGYG
                     KEN"
     gene            complement(873..1196)
                     /locus_tag="PLG78_00330"
     CDS             complement(873..1196)
                     /locus_tag="PLG78_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21580.1"
                     /translation="MSSLRRAVAIPLLPAVVLKAGLCMFLFAAKPAADVPPCHKSQKQ
                     EQCLNCCKVIPGVEVDAFSMPAIVPLASVRLEAPRWLSVQVATKALVHEDRGKTYLRT
                     RSLLI"
     gene            1289..2182
                     /locus_tag="PLG78_00335"
     CDS             1289..2182
                     /locus_tag="PLG78_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE21581.1"
                     /translation="MFFNVGAGHHHGPPTVTRAFVLGVIINSAFVVMEAAAGLFLGSI
                     ALLTDAGHNLADVGSLLVGLVAARLAEKRSSSVYTYGLGKSTVLAALINATFLLVSTG
                     AIGYAAIVRLTSPVPIAGEKVALVAFVGIIINGASALLFFKDRHHDLNVRAAYLHLLA
                     DAGISLGVVVAGFVVYLTGWMWFDPAVGILVGVAILYGAFSLMRDALRLSLDGVPPAL
                     ELSTVRDYLLSVDRVCDVHDLHVWAMSTTETALTAHLVTDDTASLSFEEINEELFHRF
                     HISHATLQVEPVGGADCRQRC"
     gene            complement(2186..2452)
                     /locus_tag="PLG78_00340"
     CDS             complement(2186..2452)
                     /locus_tag="PLG78_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21582.1"
                     /translation="MKNSILISAALAGILAGGTCKEAPKAQVSDEGKCYGVNSCKGTG
                     SCASKEHGCAGLNTCKGKAWLKMKSKECADKSGKFEPVEDGKYM"
     gene            2538..3101
                     /locus_tag="PLG78_00345"
     CDS             2538..3101
                     /locus_tag="PLG78_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HNE21583.1"
                     /translation="MESSTFWFGIVPVIAFVLLESFAGKRMAIVIALVLACAELVFSL
                     VVYGAIDELTILAFVIVFIAGAISVKTGNDLYFKLQPALVSVATAAAFFIYYYLLDRP
                     LLTFMMHKYLPSMLAQLPADRQTLFEGTMRVLSRDLIFWFLLHAAATAYAAMRLSKWW
                     WFAIRVPGLYLMLFLATFIAVRGVIPA"
     gene            3185..4156
                     /locus_tag="PLG78_00350"
     CDS             3185..4156
                     /locus_tag="PLG78_00350"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE21584.1"
                     /translation="MKQDIQTTDSALPERLRPPQTLNLVLSVASVFAWIGLMYAASRA
                     ESMWVVALIVLPFSFCILTIYSLIHECEHGLFHTDRRINEVAGILLSVFFPGSFSFLR
                     YCHVGHHSRNRSECELFELYYPHENRLKKAVFFYFLYLGGFWFSVPFASVVLLVLPWF
                     SRTWLVQRSVSASAMINGIPLRALTTIRLEGLFVIAAHAALIYFLDLNPARYAILMLA
                     QGVNWSCQQFLPHAHSPLDVIDGAYNLKVHRFYQALLLNFNLHLAHHQHPRVPWNYLD
                     RFDDPGRRRPGYFEAFARFWRGPQPISEFQAEVGVRRDSSVIRPSSM"
     gene            complement(4099..4794)
                     /locus_tag="PLG78_00355"
     CDS             complement(4099..4794)
                     /locus_tag="PLG78_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE21585.1"
                     /translation="MNVLTATNLTKSFGTGESRATVVQNIHLSVRAGETVLISGPSGS
                     GKTTLLTMLGCMARPDGGEIQIQNVNVNSLSQAGLTSFRLRHIGFIFQAFRLIPSLTA
                     IENVQLTMNLAGLHSGSRKACMLLLEELGIAAKADKKSEVLSGGEKQRVAIARAIALN
                     PGLILADEPTGSLDGASGKAATAALVKTAKEKGAGLIIVSHDPRIELFADRTLHMEDG
                     RITEESRRTPTSA"
     gene            complement(4791..5861)
                     /locus_tag="PLG78_00360"
     CDS             complement(4791..5861)
                     /locus_tag="PLG78_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE21586.1"
                     /translation="MSKFLITLIGVGLTVALVLFLLAVYQGIKEAAVVYIERSPAEVW
                     VSQKNANNLLKVSSFLRADMQRELAATQDVREVSGILKVLSATTIRGSSITMVLIGFE
                     PQSEMGIPRTIIAGTSRIHNGEIILDSAFSKRHKIQLGDKLRIRDTDFLVRGICDKTN
                     ATIAQFSFITLSDARDLIGVDGIVSFFLVGFNHGTPGQNAAVLGEKFPNVSVFAKPAF
                     INNNIVELNTGLLPVVRTISIFGTIISAVVITLMMYGSVVEKRELYAIMKAIGASTWF
                     LVGLVVKQALFASVGGALLGLCIALSVAPLLSKFVPEVALAFVYEDVLFVLAGSVLIG
                     LVSAVAPLRSISRIYPAEAFRA"
     gene            complement(6155..6506)
                     /gene="ssrA"
                     /locus_tag="PLG78_00365"
     tmRNA           complement(6155..6506)
                     /gene="ssrA"
                     /locus_tag="PLG78_00365"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6623..7000
                     /locus_tag="PLG78_00370"
     CDS             6623..7000
                     /locus_tag="PLG78_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21587.1"
                     /translation="MEQLISLVSGKVARTVFGLIIALFGVFHLMYANMMAGAVPVPGG
                     VIWVYFTGFCLIAGAVGIITNFKGQGRNAAFLTGVLVLVFAFGVQLLGVMRGGENAMM
                     YQTIFLKDIMIAMGAWVIAGTFR"
     gene            complement(7221..7667)
                     /locus_tag="PLG78_00375"
     CDS             complement(7221..7667)
                     /locus_tag="PLG78_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE21588.1"
                     /translation="MIASIFSTPEDIALTVLRVTLAVVILPHGLQKLVGAFGGYGFKG
                     TMGYLTGTVGLPSVIAFLVIFIESFGAAGVLLGVGTRVAALGVAAVMLGAAWTAHRGN
                     GFFMNWTGAQKGEGFEFHILAIGLAIVLILRGGGAFSVDQILARLF"
     gene            7857..9212
                     /gene="hemN"
                     /locus_tag="PLG78_00380"
     CDS             7857..9212
                     /gene="hemN"
                     /locus_tag="PLG78_00380"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNE21589.1"
                     /translation="MEIELLKQYSKPGPRYTSYPAATAFTPDVGAEDYSMLLRDIDPA
                     RAISLYYHIPFCRNICYFCACNVIYTKNKEKAEPYIAALTREMDLVAEQTQARLKVEQ
                     LHWGGGTPSFLSHEQMNMLMAQIYRRYDFVPGAEISIELDPRETSPEQIGTLADLGFN
                     RASLGVQDFDPSVQTAVNRIQPEDITEQAYNHLRARGFRSINFDLIYGLPLQTVDTFA
                     KTMEKVVGFRPDRISIFNFAYVPWLKKHQLRMNKEDLPSTETRLQILSYAVDRLQKAG
                     YVHIGMDHFALPDDELTVSLRDGSLRRNFQGYSTRGGTELIGFGVTSIGEFNSGYAQN
                     TKSLVDYYRAIEAGRLPTERGLILTQDDRLRKAVIRQLITGLELKFETIEQAFDVRFE
                     EYFARELEAVGEFVDQGMLRFANRSLCVTEQGRFVIRNICMLFDAYLLGQNQGRYSNT
                     V"
     gene            9214..10332
                     /locus_tag="PLG78_00385"
     CDS             9214..10332
                     /locus_tag="PLG78_00385"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNE21590.1"
                     /translation="MRENPYRLFFPLSWLGAFALAFVWIPAVLIERHVLTIPGWNAYP
                     RDLHSTLAAGVFVLPAMTGFLLTAAPRMTGSSPASMIWVVALFFCEILIPPSVLLGGT
                     AIALLGACAILSAFLIATARNAENVPSYFWIIVSGTLSGVLGAILWVLLPAERIGSNI
                     IFYGMFPQILLGASTMLVVPISGSAKRMEWRTKMLSIRLFEAAGLAGLFWVSNALYFF
                     SVNAAGMPAAIAATVWILTRVHAATPGEFHGILAKMFWTACILLCIGLIGRAFPWEFS
                     VHAAHVYWIGGLVMFLLSVSVQVLLSHGGHAMIPLPSRRTLVALLILVLLTVVTRVSA
                     PVVPAMYISHLSYAAFVFLIVWILWLLRFARFLGSAKS"
     gene            10385..12103
                     /locus_tag="PLG78_00390"
     CDS             10385..12103
                     /locus_tag="PLG78_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21591.1"
                     /translation="MRRNLFYAFSFLFCAGMILSRIWVTEDAYISFRAIENLYQGYGF
                     VYNPGERVEVTSHPLWAAILIVLRSMGLSLPMTSAVLGLFFTGLAIYQLIKRSMHADA
                     FPFAAIALCSISGFRDFSTSGLEFSLAFFLMTMFYIEVQEHGLLERPMYLAALLNLLY
                     LDRPETALLYAYYSVFFVLDLWRSTPRLASKIIPAIQWGLPAILITGSYHAFRYLYFG
                     ALFANPYYAKAGLDTYYSHGLKYLAHSLIFAPAITFALLLAASFLVLHRLRSKQKPLV
                     VGRAVRDLGAFALLTFYMIRVGGDFMAFRFWLPALAILFITVDRMFISVPRFPLPLLS
                     SRPVWTDWGALLFFAALSFWPVPMTRGFIADERPAFFSIGAGSVFGSKDNAWAARGRA
                     FRKLQDCLSYEDFWIANSQAHAHCLRGVGLGYVGVEAGPLVRIFDEQGLSDGSVARAR
                     VLMRFRPGHEHYTTLAQVVERGVLFCSSGEPGYDRVMATQAGIVIRLDPDLLATLPDA
                     RARMQELVRLKAEGSEFIPRLEKRYGVRVEDLLAQSSQWESDRLMQSKQACWKSFEGG
                     PDTYFY"
     gene            12167..12240
                     /locus_tag="PLG78_00395"
     tRNA            12167..12240
                     /locus_tag="PLG78_00395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12201..12203,aa:Arg,seq:acg)
     gene            complement(12253..13824)
                     /locus_tag="PLG78_00400"
     CDS             complement(12253..13824)
                     /locus_tag="PLG78_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21592.1"
                     /translation="MGRGLILSALTGSIALSYEILWARFYSFVSGAASNSFGILLASY
                     LFGLGLGALVAGIICRNVEGHSPELNRKLGSFVLFANLFGYIIAPAASWIVLFIPYLW
                     TLPLIVLSAGLLGAAFPLISHITIPADARAGLRLSFVYLANIAGSSIGSLLTGFVLLD
                     YFSFSTISVILVIAGILLATLISEYRDPDNTKATVPARGSDFRRYGVLLAVLLVILLS
                     KGKLFDGMYERLQWKKDMFAKGLDYRFARLYESKSGVIAIGPDGSVHGGGMYDGFLDY
                     RLKLGGWLLRPYSLSLFHPHPRRVLIVGVSGGAWTAVVNGHPQVESITAVEINKHYRQ
                     VMEDEPKMRHLLTSPKLNLVFDDGRRWLNRNPEEHFDAVVMNTTYHFRTYVSNLLSRE
                     YMTLVSSHLSPGGVFLFNTTDSKEAMRTALEVFPHCMMILNATICSKEPLEFNAKRWL
                     SVLDSYRINGEQILSGEEGRKTRAWLETLTTEIDQKDALYEREGFRSQKRMLEDCSGA
                     RIVTGDNMATEYGDH"
     gene            complement(13826..14449)
                     /locus_tag="PLG78_00405"
     CDS             complement(13826..14449)
                     /locus_tag="PLG78_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21593.1"
                     /translation="MIRSLLIFVVFFVSGISALTYQLAWQRKLFSIFGTNIESITVVV
                     TAFMMGLGLGSLAGGILSQRKNFRVLPAFAIVELGIGVYGLLSMRLMESISNASSMSV
                     AMTGIVSFALVLVPTLLMGATLPLLVTHLVRGNQNVGQSVGLLYFANTIGAAFGALIG
                     ATIWFRFYGLSGSVHIAVILNFASAIVVGLLAITEKSISGKLEAEGK"
     gene            complement(14466..14789)
                     /locus_tag="PLG78_00410"
     CDS             complement(14466..14789)
                     /locus_tag="PLG78_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNE21594.1"
                     /translation="MLGTIPDKIEYDERELRILWKDGKECRFDLLSLRKNCPCANCRG
                     GHELTARRTTGHIQSIGLTSWKKVGRYALQLSWSDNHDLGIYTYDMLREACDKGTSYM
                     PPDER"
     gene            15140..16399
                     /locus_tag="PLG78_00415"
     CDS             15140..16399
                     /locus_tag="PLG78_00415"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21595.1"
                     /translation="MLLGPAFLDFVFCFAGVRNRAFSMVAWITGLFWVSVFLFFPGLV
                     ISQTPTGFRMPLHFYAYTNYYPVFLANLFGIITYALFELLRNGLRMPVRASLMRFVLP
                     ACILWAACGFWDTTLSLLFRIPLPLSWAGGVVVNVAFLAFVTRRSQEAFRAQEKHRSF
                     MRDVGHAREIQSGLITTEFPSMHRIKIVGKYMPMQELGGDFYNVRRLDEHRLSIFISD
                     VTGHGIASAFITAMIKISLDSLPMNVLIHPDKVLNHLNEALLDKIMDRFITGIYGILD
                     EDTMEFHFCSAGHHPPALHFKAASGQVEELMARGRLLGAFDSLELVTESIPLKSGDRI
                     LLATDGLYEPFDDKGEMFGLARLKEIFHRELTHSLDPLFLAIHRYTKGARQADDMAGL
                     LILVDPVPTTRPVPSAHSQSADSSSTR"
     gene            complement(16317..17651)
                     /gene="argG"
                     /locus_tag="PLG78_00420"
     CDS             complement(16317..17651)
                     /gene="argG"
                     /locus_tag="PLG78_00420"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNE21596.1"
                     /translation="MSRIYRELPPKGTRIGIAFSGGLDTRAAVAWLAEQGMEVCAYTA
                     DLAQPDETDIASIPQIALDHGAKSARMVDCRDALAAEGLTAIQCGAFHIHTGGKKYFN
                     TTPLGRAVTGTHIVKAMREDDVHVFGDGSTHRGNDIQRFYRYGILVNPDLRIYKPWLD
                     QAFVNAFGGRKEMSDYLQKIGKPYRASAEKAYSTDCNMLGATHEAKDLEFLDRGMEIV
                     EPIMGKAFWKSDVSIEPEMVRVDFEHGVPVAINNTRKSGHDLILLANSIAGKHGLGMS
                     DQIENRVIEAKSRGIYEAPGMALLHICYERLLTSIHNENSLDQYYNHGRRLGRLLYEG
                     RWYDPEAMMMRESFRWISYPVTGSVVIELRRGDDYTIRKTESANATYHPEKLSMEKTE
                     SAFTPSDRIGQLEVQNLNISDNRQLLTEYLKNPQIANAQKELDEWLEQDRRG"
BASE COUNT         3971 a         4441 c         4478 g         4829 t
ORIGIN      
        1 gttttccttt ccataaccgg cgaacacttc aaaatcagga agatagttca tccacgcacg
       61 cgtgtcgtcc agatctcctt gtttgatctg cgactcggcc attttcattt ccagagaatg
      121 ttcctcaaac tttgcagcca gatttcgcac gcgtgtttcc aattcagcga gctcggaatc
      181 tacctgatca tcggacatgt tgtgtgtttc cccggaggac gttgtatagt attcgaccgc
      241 gcgatgaaca gacgcatatt cactctgtgc ctggttcacc gcgagagcag cttctgattc
      301 tcgaacatga accatggata gatccaccag aggtgtcttt cctgtttcgt atcgaatgcg
      361 cgcaagggtt tcaagagtgc gaagcctttc ctgcgtctgg gaattcagtt tgattcgcgc
      421 ctgaatgagc ctttcgcgcg tgcggagcat caggaattcc cgcgccatcc gattcttgat
      481 caaccggatt cgaagtgttt cttcgtcagc cttcgcaccg aggcgaaggg attctactgt
      541 gcccttacca ggaaaaggaa ttggttgacg cacttcaaat tcagatccag tgatcttctt
      601 tcgatccacc atctgatcag gaagggcctg aatcatatcc tctctgccgc gactgcgcga
      661 gatttcaatt tctgggtccg gatacccaaa catcgtgcta cgggccgcat gtctcgacgc
      721 tcctgcagtc gcagactggc tctgcaccag cggatgttga tctatgattt cctcaagcgg
      781 tgaagcatag aggttcgcag cgaaccataa cgctgcgcaa agtattagcg atttcatgtt
      841 tcctccttcg cgttttgcag gcgaaggaga ttctagatca ggagggaacg cgttcgaaga
      901 tatgtttttc cacgatcttc atgaaccaga gcttttgtgg caacctggac ggacagccag
      961 cgcggagctt ccagccgaac ggacgccagc ggaacgatag ctggcattga aaatgcgtcc
     1021 acttccacgc caggaatcac tttgcagcaa ttgaggcatt gttcctgctt ctggcttttg
     1081 tggcacgggg gcacatctgc cgcgggtttg gccgcgaaca aaaacatgca gagccctgct
     1141 tttagcacga ccgctggaag aagtggaatg gccacagctc ttcgcaagga actcatacct
     1201 tacctaatga gtcggatcaa acgatcgtca actaaaaaag ggagagcgcg attgtgcaaa
     1261 aaagcgtgac gcagcgcgaa gcaatggaat gtttttcaat gtgggcgccg ggcatcatca
     1321 tggacctcca acagtcacgc gagcgtttgt gctgggcgta atcatcaaca gtgcattcgt
     1381 tgtaatggaa gccgcggccg gattgtttct aggatccata gcccttctga cagacgcagg
     1441 acataatctt gcggacgtag gtagccttct cgtagggctc gtagccgctc gcctggcaga
     1501 gaagagatcc agttctgtat acacctacgg tctgggcaaa agcacagtac tggccgcttt
     1561 gatcaatgct actttcctgc tcgtttccac gggagccatt ggctatgcgg cgattgttcg
     1621 cctaacgtca ccggtaccga tcgctggaga gaaggtggct ctcgttgcgt ttgttggaat
     1681 catcatcaac ggagcgagcg ctctcttgtt tttcaaagat cgacatcacg atctgaacgt
     1741 gcgtgccgcg tacttgcatt tgcttgcgga tgctggaatt tcactgggag tcgttgttgc
     1801 tggcttcgtg gtgtatctaa ccgggtggat gtggtttgat cctgcagtcg gtattctggt
     1861 tggggttgcc attctttatg gagctttttc tttgatgcga gatgcgcttc gtctgtctct
     1921 ggacggtgtc ccgcctgcac ttgagctttc aacggtgcgc gattatcttc tttctgtaga
     1981 tcgagtttgt gatgttcacg atcttcacgt ctgggctatg agtactacgg agactgctct
     2041 gacagcacat ctggtaacag acgatacggc gtcgctttcc tttgaagaaa tcaatgaaga
     2101 gctatttcac cgatttcata tttcgcacgc aacgctgcag gtagagcctg ttggcggggc
     2161 ggactgccga cagcgttgct aagtattaca tatatttccc gtcttccact ggctcgaact
     2221 ttccactctt gtctgcgcat tctttgcttt tcatcttcag ccaggctttg cctttgcaag
     2281 tgttcaaccc ggcacaaccg tgttctttgc ttgcgcagga accagttcct ttgcaggagt
     2341 tcactccata gcatttgcct tcgtcactga cctgtgcttt cggtgcctct ttacaggtgc
     2401 caccggcaag gatgcccgcc agcgcggccg aaatcaaaat cgaattcttc atgcattcaa
     2461 ttcagtgaat gcgcctcttc cgtaaagtga aatacacccg ggattgacag cacgcgcggg
     2521 gcttttcacc tctggttatg gagtcctcta cattctggtt cggcatcgtt cctgtgattg
     2581 cgtttgtgtt actggaatcc ttcgccggaa agaggatggc gattgtgatc gcactggtcc
     2641 tggcctgcgc ggaactcgtg ttctctctgg ttgtatacgg cgcgattgat gaacttacga
     2701 ttctggcgtt tgttattgtt ttcattgccg gtgcgatttc agtcaagacc gggaatgatc
     2761 tatattttaa attgcaaccg gcattggtga gtgtcgcgac cgccgctgct ttctttatct
     2821 attactatct tcttgatcga ccattgttga ccttcatgat gcacaaatac ttgccatcga
     2881 tgcttgcgca attgccggcg gatcggcaga cattgtttga aggaacgatg cgagtcttgt
     2941 ctcgcgacct tatcttctgg ttcctgttgc atgcagcggc cacagcgtat gctgcgatga
     3001 ggttgtctaa atggtggtgg tttgcgattc gcgtgcccgg cctctacttg atgctgttcc
     3061 tggccacatt cattgcagtt cgtggagtga tcccggcctg aggtgattct ctgttcgatt
     3121 ccctcacgcg taaaaaaaat ctgctggaag aaatccctgg gccagtctcc catgaaacag
     3181 aagcatgaag caagacatcc agacaacaga tagtgcactg cctgaacgtt taaggccgcc
     3241 tcaaacatta aaccttgttt tatccgttgc gtctgttttt gcctggattg ggttgatgta
     3301 tgccgccagt cgggcagagt ctatgtgggt ggttgcgctg attgttttgc ctttttcttt
     3361 ttgcattcta actatctatt cgctcatcca tgaatgcgag catggcctgt ttcatacgga
     3421 tcgtcgtatc aatgaagtcg cgggtatttt gctttctgtc ttctttccag gatcattttc
     3481 atttctacgc tattgccatg tgggccatca cagcaggaat agaagtgagt gtgaactttt
     3541 tgaactctac tatcctcatg agaatcgact gaagaaggct gtatttttct atttcttgta
     3601 cctgggcgga ttctggttca gcgttccgtt cgcatcagtc gtgcttctgg ttttgccgtg
     3661 gttttctcgc acctggttgg ttcaacgttc tgtttccgca tctgcgatga tcaatggaat
     3721 accattgcgg gctctcacta cgattcgcct tgaagggctc tttgtgattg ccgcacatgc
     3781 agcattgatc tactttctag atttgaatcc cgcacgttat gcgatattga tgcttgcaca
     3841 gggagtgaac tggtcctgtc agcagtttct gcctcatgct cattcccctc tcgatgtcat
     3901 tgacggtgcg tacaatttga aggtgcaccg gttttatcag gcactgttat tgaatttcaa
     3961 tcttcatctg gcccatcatc agcatccgcg agtaccgtgg aactacctgg atcgatttga
     4021 tgatcccggt cgtcgtagac cgggctactt tgaagccttt gccaggttct ggcgtggtcc
     4081 gcagcccatt tctgaatttc aggctgaagt gggggtccgg cgtgattctt ctgtgattcg
     4141 accatcttcc atgtgaagtg ttcgatctgc aaagagttcg atgcgtgggt cgtgactcac
     4201 gatgatcaat cctgctccct tttcttttgc agtctttaca agtgcggccg ttgcggcttt
     4261 ccctgatgcg ccatccagac tacccgtagg ctcatctgcc agaatcaaac ctggattgag
     4321 cgcaatcgcc cgagcaatag cgactctttg tttctctccc ccgcttagaa cttcagattt
     4381 tttgtccgcc tttgcggcaa taccaagttc ttccagaagc aacatgcatg cttttcgcga
     4441 cccggaatgc aggcccgcta gattcattgt gagttgtaca ttttcaattg cggttagcga
     4501 tggaatcaaa cgaaatgctt ggaaaataaa accaatatgg cgcaggcgaa atgaagtaag
     4561 gccagcttga gacagcgagt ttacatttac attttgaatc tggatttctc ccccatcagg
     4621 tctggccatg catccgagca ttgtgagaag tgttgttttt ccgctgccgc ttgggccgga
     4681 tataagtacc gtttcaccgg cgcgaacgct gagatgaatg ttctggacaa ccgtagcgcg
     4741 agattctcct gtcccaaagc tctttgtaag attggtcgct gtcaacacgt tcatgcgcgg
     4801 aatgcctccg ccggataaat gcgcgaaata cttcggagcg gagcaaccgc gctgacgagt
     4861 ccaatcaaaa cggatccagc gagcacaaag agaacatcct catatacgaa agcgagggcc
     4921 acttctggca cgaacttgga caaaagcggt gctacagaca gagcaatgca caggccgaga
     4981 agagctcctc cgacggatgc gaacaaagcc tgcttcacca caagtccaac cagaaaccag
     5041 gtagaggctc cgattgcttt catgatcgca tatagttcgc gcttttcaac gacgctcccg
     5101 tacatcatga gtgtgatgac tactgcgctg atgatcgtcc caaaaatcga aatcgtgcgt
     5161 actacgggta ggagacctgt gttgagctcg acaatattgt tgtttatgaa tgcgggtttt
     5221 gcaaatacag aaacgttagg aaacttttcg ccaagcactg cggcattttg ccccggtgtt
     5281 ccatggttga atccaaccaa aaagaaactc acgatgccat ctacgccaat cagatctctc
     5341 gcgtctgaaa gagtgatgaa cgagaattga gcgatcgttg cgttggtttt atcacagatt
     5401 cctcttacaa gaaaatcggt gtcacgaatt cggagtttgt ctcccagttg aattttgtgg
     5461 cgctttgaaa acgcgctatc tagaatgatt tcaccgttgt gaattcgtga ggttccggca
     5521 atgatcgtgc gagggattcc catttccgat tgtggctcga acccgatcag caccatggtg
     5581 atcgaacttc cccgaatcgt agtggcagag agcactttca agatgccgct aacttcgcgt
     5641 acgtcctggg ttgcagctaa ctcccgttgc atgtctgctc gcaggaagct tgaaactttc
     5701 aggagattgt tcgcattttt ttgactgacc cagacttctg ctggactcct ctcgatatag
     5761 acgacggccg cttctttgat gccctggtag actgcaagca ggaacaaaac cagagcgacc
     5821 gttaatccca caccgatcag agtgatgaga aacttactca aatcgcctga aatgaatctt
     5881 cgaacgattg ggatcaaatc tgtttaccca tctgcatccg ccttctcctg agagtggatg
     5941 cggtgatttt tctgtctacg cattctgttt tcggcagcgg tacacattac gaactcgaac
     6001 gatagtttct ggtatccgaa agctgagacg cggcgggacg attccgagcc tccatacgag
     6061 cgggcagggg cacgaaagcg aagccaggag ggcgaagcgc accacggctc gggcacggac
     6121 gctcgcgccg tggtggcgtg ccccctgccc gttcctatgg agacggcggg agtcgaaccc
     6181 gcgtcctacg gtgcgaagca tgtgcttcta cgtgcgtatc cagcaattaa taatcccggc
     6241 cgatgcctgc tggaggggcc tgtccgagat gctcaatctg tattagtttg ccgtaccgca
     6301 gattgaaccg gtccgactca tcctcccaaa gtttgacact cttctacaat ccggaaggag
     6361 ggactgcagg gagcgctggt cgcttaaatt aagcggctag agccatttcg ttgttggcag
     6421 ttacaagttt gaagttttta agagctcttc gactctgcac gcaacacaca ccccgaaaac
     6481 acagcagtcg aagccaattc gtccccgtgg tgtatcgtat gcattataag gcattcatgg
     6541 aagcaaagca agcgaaattt tcgcagtttg ctttgacgcg aaacacataa cagagtatcc
     6601 ttcaaccaaa aggagacatt gtatggaaca gttgatttct ctggtgtctg gcaaagtggc
     6661 gcgtacagtt ttcggactga tcatcgcttt gttcggcgtc tttcacttga tgtacgcgaa
     6721 catgatggcg ggcgcagtcc cggttccggg cggagtgatc tgggtgtact tcacagggtt
     6781 ttgtctgatt gctggcgcgg tgggtatcat cacgaacttt aagggacagg gccgcaatgc
     6841 agcgttcctg accggtgtgc tcgtgctggt gtttgcgttt ggcgttcaac tactcggtgt
     6901 tatgcgaggc ggggaaaatg cgatgatgta tcagaccatt tttctcaaag acatcatgat
     6961 cgccatggga gcgtgggtca tcgcgggaac cttccgctag gacgtgcgtg atctgtcgcc
     7021 ctgattttgt ctctagaatc agggcgattc ctatttcccg actggtacat tggatatcgt
     7081 cgcaagactc gcaaagcaag cataccggag catcgtatgc aattgatttg gatcgtcaag
     7141 cgatgccata aggtggagcc aggaatagct tgcgccggat tcgtacggcg caaaatatct
     7201 gttaggtgaa gattattgtt ttagaagagc ctggccagga tctgatctac gctgaaggcc
     7261 cctccgcctc gaaggatgag cacgatagca agtccaatcg caaggatatg aaattcaaat
     7321 ccttctcctt tttgtgctcc cgtccagttc atgaagaaac catttcctct atgcgcggtc
     7381 caggcagctc caagcattac agcggctaca ccgagagctg cgacccttgt gccaacaccg
     7441 aggagcactc cggccgctcc aaatgattcg atgaaaatga cgagaaatgc gatcacagag
     7501 ggcagtccaa ctgtgcccgt cagatagccc atggttcctt tgaacccgta accaccaaaa
     7561 gcacctacta gtttctgcaa gccatggggc aaaatcacaa cggccagagt gacgcgcagg
     7621 acagttagag cgatgtcctc cggcgtcgaa aagattgaag cgattatcat aatgtaaacc
     7681 ttccttttat gaagctcaaa ataatgcttt aatgttcaat cgtcaaggtt taatgggatg
     7741 aaatattcct tttttctttg gatttgatgt cagtcaattg ccgcgccgct agaatcaact
     7801 ttcaggtatg agaaatatca tagcaggaac tgccgccgag ggggcatact agcgacatgg
     7861 aaattgaact gttgaaacaa tacagtaaac cgggaccgcg ttatacaagc tatcctgcgg
     7921 ccactgcttt cacgcccgac gtgggggcag aggattactc gatgcttcta cgtgacattg
     7981 acccggcccg cgcgatttct ctttactatc acattccttt ctgtcgcaat atctgttact
     8041 tctgtgcctg caacgtcatc tacacgaaga acaaggaaaa ggcagagcca tacatcgcgg
     8101 ctctcactcg cgagatggac cttgttgcgg agcaaaccca ggcacgactg aaggtagaac
     8161 aacttcactg gggtggtgga actccgtcgt ttttgtcgca cgagcagatg aacatgctga
     8221 tggcgcagat ctacaggcga tacgatttcg ttccgggcgc ggagatcagc attgaactgg
     8281 accctcgcga aacatcacca gaacaaatcg gcacgctggc cgatctcgga ttcaatcgcg
     8341 cctcactggg cgttcaggat tttgatcctt ccgtacaaac cgcagtcaat cgaattcaac
     8401 ctgaggatat aacggagcaa gcatacaacc atctgcgcgc acgcggattt agatccatca
     8461 acttcgatct catctatggg cttccccttc aaaccgtcga cacgtttgca aagacgatgg
     8521 agaaggtagt tggatttcgg ccggatcgaa tttcaatttt taacttcgca tatgttccgt
     8581 ggctgaagaa gcatcagctc cgcatgaaca aagaagacct cccatctaca gagactcgcc
     8641 tgcagattct ttcgtatgct gtagatcgtc ttcaaaaagc aggatacgtt cacattggca
     8701 tggaccattt cgcactgccg gatgatgaat tgaccgtgtc cctcagggat ggcagcctgc
     8761 gaagaaactt tcagggctat tctactcgag gcggcacaga gctcattggg tttggggtca
     8821 cctccatcgg agagtttaac agtggttatg cgcaaaacac gaaaagtctt gtggattact
     8881 atcgtgccat tgaggctggc cgactaccaa cggagagagg tttgattcta actcaggatg
     8941 atcgcttgcg caaagccgtg attcgccagt tgattacggg ccttgagctg aagttcgaga
     9001 caattgaaca ggcttttgac gttcgctttg aagaatattt tgccagagaa ctggaagccg
     9061 ttggcgaatt tgtagatcag ggaatgctca ggttcgcgaa tcgatctttg tgtgtgaccg
     9121 aacagggtcg atttgtgatt cgcaatattt gcatgctgtt cgacgcctac ttgttgggcc
     9181 aaaaccaggg tcgatatagc aacacggttt agaatgcgag aaaatccata ccgtctgttc
     9241 tttccattgt cctggctggg agcatttgcc ctggcttttg tatggatccc ggcagttttg
     9301 attgagcggc acgtcctgac gattccaggg tggaatgcat atccccggga tttgcacagt
     9361 actcttgccg ctggtgtgtt cgtattgccc gcgatgacag ggttcctgct tacagcggcc
     9421 ccgaggatga caggctcttc gcccgcatcc atgatctggg tcgttgccct gttcttttgt
     9481 gaaatattga ttccaccttc ggtcctgtta ggtggaactg cgatcgcgct tctgggagct
     9541 tgcgcgatat tgtctgcgtt tttgattgcg acagccagga acgcggaaaa tgtcccttct
     9601 tatttctgga tcattgtatc ggggacccta tccggagttc tgggagcgat cctctgggtt
     9661 ttgcttccgg cagagcggat tggctcgaac atcatctttt acggtatgtt ccctcagatt
     9721 ctactcgggg cttccacaat gctcgtcgtt cccatcagtg gctctgcgaa gagaatggaa
     9781 tggcgcacaa agatgttgag cattcgattg tttgaggcag cggggctggc tggccttttt
     9841 tgggtttcaa acgcgttgta ctttttttcg gtaaatgccg caggaatgcc ggcagcgatt
     9901 gcagccacgg tgtggatcct gactcgggtg catgccgcca ctccgggtga gtttcatgga
     9961 atcctcgcaa agatgttttg gacggcgtgt attctgttat gcattggctt gattggcaga
    10021 gcctttccgt gggaattttc ggtgcatgcg gctcatgttt actggattgg gggcctggtc
    10081 atgtttctcc tttccgtatc tgttcaagtc ttgctttcgc acggcggtca cgccatgatt
    10141 cctcttccct cgcgcagaac gttagtcgct cttctgattc tggtattgtt gaccgttgtt
    10201 acccgcgtgt cggcgccagt cgttcccgca atgtacatct ctcacttgag ttatgccgct
    10261 tttgttttct tgattgtatg gattctctgg ttgctgcgat tcgcgcgatt tctgggcagt
    10321 gccaaaagct gacccggagt ttatttttta ctttgcacga tcctgtcccc tcttaagtca
    10381 agctgtgcgt cgtaacctct tctatgcatt cagctttctg ttctgcgcag gaatgatttt
    10441 aagcagaatc tgggtaacag aggatgcata catctccttc cgggccatcg agaatcttta
    10501 tcagggctac ggatttgtat ataatccggg agagagagta gaagtaacca gtcatcctct
    10561 gtgggctgcg attctcattg tcttgcgcag catgggactc tcgctgccta tgacttctgc
    10621 agtcctcgga ctctttttta cgggtctggc catctatcaa ttgatcaaaa gatcaatgca
    10681 tgcggacgcg tttccatttg ctgcgattgc actttgttcc atctccggat tcagagattt
    10741 ctctacgtcg ggcctggagt tttcgctcgc gttttttctg atgaccatgt tctacatcga
    10801 ggttcaggag catggactgc tcgaaagacc catgtatctg gccgcactgc tgaatctgct
    10861 ttatctggat agaccagaga ctgcgcttct atatgcgtac tattctgtat tctttgttct
    10921 cgatctgtgg agatccacgc ctcgcctggc ctctaaaatc attccggcaa ttcagtgggg
    10981 actccctgcg attctgatca caggatctta tcatgcgttc cgctatttgt atttcggagc
    11041 gctttttgcc aatccatact acgccaaagc cggcctggat acgtactatt cccacggtct
    11101 aaagtatctg gcacattcgc taatatttgc tccggccatc accttcgcgt tgttactcgc
    11161 tgcttcattt cttgtgctgc acaggctgcg atccaaacaa aagccattgg tggttgggcg
    11221 tgcggtcaga gacctggggg cttttgcact cttgactttc tatatgattc gcgtgggcgg
    11281 agattttatg gcgttccggt tctggttgcc tgcccttgcg attttattta tcactgtgga
    11341 tcgtatgttc atttctgttc cgcgatttcc actgcctcta ctgtcgtcgc gtcctgtctg
    11401 gactgactgg ggagccctgc tgtttttcgc agcgttgagt ttttggccgg tgccaatgac
    11461 cagaggtttt atcgcagatg aaaggccggc cttctttagc attggagcag gcagcgtgtt
    11521 tggctcaaaa gacaacgcat gggccgcaag aggtcgcgcc tttcgcaaac tgcaggattg
    11581 tctttcttac gaagatttct ggatcgcaaa cagtcaggct cacgcgcatt gcttgagggg
    11641 cgtaggtctg ggttatgtgg gagtagaggc gggacctctt gtacgtatct ttgatgaaca
    11701 gggattgagc gatggttctg ttgcacgagc gcgagtgctc atgcgttttc gtccgggcca
    11761 cgaacattac acaaccctgg cacaggttgt ggaaagagga gtattgtttt gcagttcagg
    11821 agaacctgga tatgatcgcg tgatggccac gcaggcaggg attgtcattc gcctggatcc
    11881 tgatctgctc gcaaccttgc cggatgccag agcgagaatg caggagctgg ttcgcctcaa
    11941 agcggaagga agcgaattca ttcccaggct tgagaagaga tacggcgtgc gagtagaaga
    12001 tttgctggct cagtcttctc agtgggaatc agacagactc atgcagagca agcaggcatg
    12061 ttggaaatct tttgaaggtg gtccggacac ttacttctat taggcttgcc tctcacagtc
    12121 gaaaaccgtt cgacactaaa acgcaggtcc gaaaaatggc cctcacgcgc ccatagctca
    12181 gctggataga gtgcctggct acgaaccagt aggtcggagg ttcgaatcct tctgggcgca
    12241 aatttttttc tcttaatgat ctccgtactc cgtagccatg ttatcgcctg tgacgatgcg
    12301 tgcaccagaa caatcctcta gcattcgttt ttgacttctg aatccttctc tctcatagag
    12361 cgcatctttc tgatcaattt cagtcgtcag agtctcaagc catgcgcgtg tctttcttcc
    12421 ttcttcaccc gaaagaatct gttccccgtt gatcctgtaa ctgtccagaa cagacaacca
    12481 tcgcttagcg ttgaattcaa gtggctcttt gctgcaaatg gtcgcgttta gaatcatcat
    12541 gcaatgagga aatacttcaa gcgctgtgcg cattgcttct tttgagtccg tagtattgaa
    12601 aaggaaaacc ccgcctggag atagatgcga actcacaaga gtcatgtatt ctctggagag
    12661 taagttactc acgtacgtgc gaaagtgata cgttgtgttc ataaccactg cgtcgaagtg
    12721 ttcttccgga tttcgattga gccatcgacg accatcgtcg aagaccagat tgagtttggg
    12781 gctggtgaga agatgccgca tcttaggttc gtcttccatc acctggcgat agtgcttgtt
    12841 gatttctact gcagtgatgg attctacctg cggatgtcca ttgacaacag cagtccacgc
    12901 gcctcctgac actccaacga tcaaaacgcg acggggatga ggatgaaaca aagaaagaga
    12961 ataaggtctc agtaaccagc ctcccagctt caagcggtag tccagaaaac cgtcatacat
    13021 tccgccgcca tgaacagaac catcggggcc aattgcgatt acgccgctct tgctttcgta
    13081 gagtcgcgcg aagcgatagt ccagaccttt cgcgaacatg tccttcttcc actggaggcg
    13141 ttcgtacatt ccatcaaaga gtttcccttt tgacaaaagg atcacaagta gtacagcaag
    13201 caagactccg tagcgtctga agtcggatcc gcgcgcaggg accgttgctt tcgtgttgtc
    13261 cggatctctg tattctgaaa tcagcgtggc aagcaagata cccgcaatca cgaggatgac
    13321 tgaaatagtt gagaaactaa agtaatcgag cagcacaaat cccgtcagca agcttccaat
    13381 ggaggatccc gcgatgtttg cgagatagac aaagctcagg cgaagcccgg ctctcgcgtc
    13441 tgccggaatc gtaatgtgac tgatcagcgg aaatgcagca cccagcaacc ctgcgctgag
    13501 tacgatgaga ggcagagtcc acaggtacgg aatgaataaa acgatccagc ttgccgccgg
    13561 ggcgatgata tatccaaaca gatttgcaaa cagaacaaac gatcctagct ttctgtttag
    13621 ctcgggcgaa tgtccttcca catttctgca aatgattccc gcaaccagtg cgcccagtcc
    13681 cagaccaaaa agatagctgg ccagaagaat tccaaaactg ttggatgcag caccggaaac
    13741 aaaagagtag aatctggccc agagaatttc ataggatagt gcaatggaac ctgtgagagc
    13801 ggatagaatc aatcctctgc ccatattact ttccttccgc ttccagtttt ccggagattg
    13861 acttttccgt tatggccaat aatcccacga ctatggcaga cgcgaaattc aagatcactg
    13921 ctatgtgcac agatccactc agaccataaa agcgaaacca gatggtggca ccaatgagag
    13981 ctccaaatgc agccccgatt gtatttgcga agtaaagtaa ccctacggac tgacctacat
    14041 tttgatttcc tcgaactaga tgagtcacga gaagtggaag tgtggcgccc atcaagagtg
    14101 tgggaaccag caccagtgca aatgatacga tgcccgtcat cgcgacgctc atgctagagg
    14161 cattgctgat tgattccatc agtcgcatag agaggagccc gtatactccg atgccaagct
    14221 caacgattgc aaatgcaggc agtacgcgaa aattctttct ctgcgaaaga atcccgccgg
    14281 ccagactccc cagccccaat cccatcatga atgcagtcac tacaacggta atggattcga
    14341 tattcgtacc aaagatggaa aatagttttc gctgccatgc aagctggtag gtgagcgcgg
    14401 atatcccgga gacaaaaaag accacaaaaa tcaacaacga tctgatcatg tttcttctct
    14461 tcacatcatc tctcatccgg aggcatgtaa gacgtaccct tgtcgcaagc ctctcgcagc
    14521 atatcgtagg tgtagattcc aagatcgtgg ttgtccgacc aggagagctg taaggcgtat
    14581 ctacccactt tcttccacga agtcaggcca atcgactgga tgtgcccggt tgtccttcgc
    14641 gctgttagtt catgtccgcc ccgacagttc gcgcacgggc agttctttcg caggctgagt
    14701 agatcaaatc ggcattcttt tccatctttc cagaggatgc gcaattcgcg ttcatcgtat
    14761 tcaatcttgt ctggaattgt tccaagcatg agggccagtt agcccctggt gcactctcag
    14821 tcaatgaatc acgcagaaat cattttgtaa aactgcttaa actgcttgac cgtcatcgtg
    14881 aatgcgtcat aaaaacggcg tcgatggagc taatcacact ggcgtctttc ggatttgcat
    14941 gggcttccgg agccgcgttt ctcattgcgg tgtttgccgc attcatgtcc acgggtcgga
    15001 gcgaaaatga gctctggagg agattcgctt ttctttcggc agtcaatacc gttttctgcg
    15061 cggtaagtgt tgtccagaac catccagaga atttggaatc catggcgttt ttcacgcggc
    15121 tcaatctggc cggtgcggaa tgcttctggg gcctgcgttt ctggacttcg tgttttgttt
    15181 cgcgggggtt cgcaatcgag ctttttccat ggttgcgtgg atcaccggcc tgttctgggt
    15241 ttctgtgttt ttgttttttc ccgggcttgt catttcacaa actccgaccg gatttcgcat
    15301 gccccttcat ttctatgcat ataccaacta ttatcccgtc ttccttgcaa acctctttgg
    15361 aatcataaca tacgctctct ttgaacttct tcgcaacggt cttcgtatgc cagtgcgcgc
    15421 atcgctcatg cgatttgttt tgcctgcctg tattctctgg gctgcatgcg gtttctggga
    15481 tacaaccctg tctctgcttt tcagaattcc cttaccgctc agctgggccg gtggagtagt
    15541 tgtgaatgtg gcgttccttg ctttcgttac gcgtcgcagc caggaagcgt tccgggctca
    15601 ggagaaacat cgttcattca tgcgcgatgt gggtcatgcg cgcgagattc aaagtggatt
    15661 gattacaact gaatttcctt ccatgcatcg catcaagatt gttggaaaat acatgcccat
    15721 gcaggagctc ggaggagatt tttataacgt taggcgactg gacgagcata ggctgagcat
    15781 cttcatcagc gacgtaacgg gacacggcat tgcaagcgca ttcatcactg caatgatcaa
    15841 gatttccctc gatagtctcc caatgaatgt actgattcac ccggacaagg tcttgaatca
    15901 tctaaacgaa gcgttgctcg ataagatcat ggatcgattc atcacgggaa tctacggaat
    15961 cctggacgaa gacacgatgg aatttcactt ttgctctgcc ggacaccatc ctcctgcttt
    16021 gcatttcaag gccgcctcgg ggcaggtgga ggaactgatg gccagagggc gattgctggg
    16081 tgcgttcgat tcactggagc ttgtgactga atcgattcca ctgaaatcag gggatcggat
    16141 tttgctcgcg acagatgggc tctacgaacc atttgatgac aaaggggaaa tgtttggcct
    16201 cgcgcgatta aaggaaatct ttcatcgcga actaactcat tctctggatc cactttttct
    16261 ggcgatccat cgttatacga agggcgccag gcaggcagac gacatggccg gtcttcttat
    16321 cctcgtcgat cctgttccaa ccactcgtcc agttccttct gcgcattcgc aatctgcgga
    16381 ttcttcaagt actcggtgag caattgtctg ttgtcagaga tgttcaggtt ttgaacttcc
    16441 agttgtccga ttcgatctga aggagtgaac gcactttccg ttttctccat agaaagtttt
    16501 tccggatggt aggttgcatt cgcggattcc gtcttgcgaa tcgtataatc atctccgcga
    16561 cgaagttcaa tgactacgga cccggtcaca ggataagata tccagcgaaa ggattcgcgc
    16621 atcatcatag cttccggatc ataccagcgt ccttcataaa gaagtctgcc caggcggcgg
    16681 ccgtgattgt agtactggtc cagtgagttt tcattgtgaa tggaagtgag tagtctttcg
    16741 tagcaaatgt gcagaagtgc cattcctggc gcttcgtaga tccctctgct ttttgcttca
    16801 atgacacggt tttctatctg gtccgacatc ccaagtccgt gcttgcctgc gattgagttt
    16861 gcaagaagga tgagatcatg tccgctcttt cgcgtgttgt tgatagcgac gggcacaccg
    16921 tgttcgaaat cgactcgcac catctctggt tcaatggata cgtccgactt ccagaacgct
    16981 ttgcccataa tgggctctac aatttccatc cctcggtcca ggaattccag gtcttttgct
    17041 tcgtgagtgg cgccgagcat gttgcaatcc gtggagtatg ctttctccgc actcgcacgg
    17101 tatggttttc caattttttg caagtagtcc gacatttctt ttctaccgcc gaacgcattc
    17161 acgaatgcct ggtccagcca gggtttgtag attcgcaggt cgggatttac caggatgcca
    17221 tatctgtaga atctctgaat gtcattgccg cggtgagtac ttccatcgcc gaacacatgc
    17281 acgtcatctt cgcgcattgc tttgacgatg tgtgtgcccg ttacagccct tcccagtggc
    17341 gtcgtattga agtatttttt tccgccggtg tgaatatgaa aagcaccgca ctgaatcgca
    17401 gtgagacctt ctgcagccag tgcatcgcgg cagtccacca tgcgcgcact ctttgctccg
    17461 tgatccagag cgatctgcgg aatagaagcg atgtctgttt catcgggctg cgcaagatct
    17521 gcggtgtacg cacagacttc cattccctgt tctgccagcc acgcaacggc ggccctggta
    17581 tcaaggccgc ccgagaaagc gattccgatt cgtgtacctt ttggcggaag ttctctgtaa
    17641 atgcggctca tgagccatta ttttcatttg ttcaaagaac tcaaattctt tcgcatcacg
    17701 gaagaattcg gactattgc
//
LOCUS       DAOQJL010000005        17660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1515_length_17660_cov_4.365408, whole genome shotgun sequence.
ACCESSION   DAOQJL010000005 DAOQJL010000000
VERSION     DAOQJL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17660
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_17660_cov_4.365408"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLG78_00425"
     CDS             complement(<1..474)
                     /locus_tag="PLG78_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21597.1"
                     /translation="MARPREFDSDDALQASVNLFWEKGYHATSITDLTEAMGVLRGSL
                     YKAFGDKHTLFIKSLKAYSENLLEGMRKNLETGKPVEALNRTLDIVVGTAHGKAARKG
                     CMIANSALELVPEDPVTAVCVNKHYDAMESLLTRALEKARTDGKLKTEMSPTEAAQ"
     gene            complement(557..1507)
                     /gene="sigJ"
                     /locus_tag="PLG78_00430"
     CDS             complement(557..1507)
                     /gene="sigJ"
                     /locus_tag="PLG78_00430"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HNE21598.1"
                     /translation="MTHTSLYTEHRRLLFSIAYRMTGSSSDAQDILQEGYLKFQNVAL
                     NAVNNSRAFLITLITRMCIDFLDSAYERRRTYIGPWLPEPITESPEEDYVRMESLSTA
                     FLLMLERLSPVERAVFLLRDVFDVDYAEIASMLEKSEENCRQISSRARKHVSSEAIRF
                     ETPPQKKDQLFRAFLRAVQKGDVHSLGKLLCEDARMISDGGGKVNAARNVVSGAAKCI
                     RGILGVAKKQPPDAWPVLVEGGGEPALVLLTGGKVYTILNLSYRNGQIDAVYSILNPD
                     KLQNVKVPPLARVASIVFSYGLIPFRTVFGLVARALSRAK"
     gene            complement(1504..2724)
                     /locus_tag="PLG78_00435"
     CDS             complement(1504..2724)
                     /locus_tag="PLG78_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE21599.1"
                     /translation="MKDSIVIGGGLAGLIAANIAARAGLSVTVVEKSKSLGGRARSAV
                     RDQFIFNQGPHALYIRGNLARTLSDLGIKVTGKKGAPRGTYIGRESEFASMPTNVLGL
                     LTHPWFSRKGRVDMIRFYGRAIMGRMNGSALAFELDQIKDAKAKGFASSLVRLSTYNA
                     EPERIVAHAAADQLKLATGGVLYLDGGWQTIVDGLENSARALGVEFLTGQKVEQIESS
                     RDEVRLHTTDRIIESRTAILAADPESENAWLGTKIGSGKKLRAACLDVALDRLPEPDH
                     IFAQDIAEPLYLSVHSASANLGPGQVIHVARYLKEGEEAGEGTKKQLRDFLARFQPGS
                     EDRIVQERFLPDMIVAHDFQDTTEQPGVEIDSAKPLFRAGDWVRGSGMLSDRSAFTAV
                     TAAEKAMARIRAKS"
     gene            complement(2803..3267)
                     /locus_tag="PLG78_00440"
     CDS             complement(2803..3267)
                     /locus_tag="PLG78_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21600.1"
                     /translation="MRVLPTVVGQDKCKEDPVVGTWIALTTHGERKQHLTLRIDENKA
                     GKLRGLLLNLSYSPNLNPSQPPSHCENPDHIIRFKLPGDGTLSGNDFFFRTIELTGTE
                     RICMDPTQPDWNYEMDRFKGRLDKKAGTITVIWDDDVYVIHKPIIFRRVSCT"
     gene            complement(3326..5137)
                     /locus_tag="PLG78_00445"
     CDS             complement(3326..5137)
                     /locus_tag="PLG78_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE21601.1"
                     /translation="MKPFQSFALSAILTAFVYSIGCNPAQSRVPGEFSWTQFDEILRF
                     ARQNYIDPDNIKDNRAYVGAAESALRSLPNPLILYPRKYYQDLRAKQPEDKKIPGNIV
                     EIEKGAAYVVLAPDYSAWDKKQDALAAKERERRKKISSKQRQEEVEKQRKEIMAEQET
                     LEKAWNDVGFGRKDFETVIAWIEKNKGQYSTLPPSYKGENPYRDEPFGIQHVFFASAN
                     GFLQTMDPHSAVMDMESWQKILKESEDSSFEGIGAMLRGGGNQEVVVETPLPGSPAHN
                     AGIRSGDVIVKVDGNSIENMVLGEVVKRIRGPKETFVTLEIERKTEVANLIIRIKRGV
                     IVQKSVSSSYIEDGKIGVIKLSSFLFSEKRPTELIRHEYESLVKKAGGRLGGLVLDLR
                     GNPGGDLDEAIRIAGLFVPENRVVVQIKRRGDKEERRADESRMIPADLPMIVLINAGS
                     ASASEIVASALMDHNTALVLGERSFGKATVQSLQPRGKILIKLTTARYYAPNGYTIQV
                     YGVQPDIAVSEEEDNGFPPRFREEDMWKHLPELTARDRNPAREAWIQKIKAAVGNNEK
                     AEQEIKLHKGDALKPDYMMLRALSYFNALKQQPSPTE"
     gene            5166..6110
                     /locus_tag="PLG78_00450"
     CDS             5166..6110
                     /locus_tag="PLG78_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21602.1"
                     /translation="MGSFMQGGSLASILFILFCGCSTAPVQDRPVHDRLDAYELAFHL
                     YFREERKENAIDLLSWACRSLEDGRYISCYDLGQLLLREGRKDDAARAFAESYRISPN
                     GPALSALYDLWATGTGPTPPPAPALVVISKTVERFCRAGDRQAAMIALKSSPADIPRE
                     LFAQPFFQECLFNQPEYAPFIAGRTPRPVPIDEIYRQRAAVHPLHAVLDVAPYLKAEY
                     LAFESNHPVNVSWKLFLNAVRARNQVAAESALANFYAALEKQRVPGGAADSQILAMKR
                     AAGTLIAGDIFFASIRGSARIQKMVRDTLPGSGIFASK"
     gene            6145..6306
                     /locus_tag="PLG78_00455"
     CDS             6145..6306
                     /locus_tag="PLG78_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21603.1"
                     /translation="MKRPHNRIEKITNNPKVVSLALYRIKKGLKKEGFELITDEQGKL
                     KLVMRKNKS"
     gene            6375..6860
                     /locus_tag="PLG78_00460"
     CDS             6375..6860
                     /locus_tag="PLG78_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE21604.1"
                     /translation="MKIEKHKVVSIDYTLKDDQGNIIDSSRGGEPLAYIQGVGNLIPG
                     LESALEGGSKGDRLSVSVPPERGYGVLEDHLIHVLRRDQFEGVEELEVGMQFHAHADN
                     GAVQTVVVTKIDGDNVTIDGNHPLAGKNLHFDVAVVEVRDATAEELEHGHVHGPGGHH
                     H"
     gene            complement(7000..7359)
                     /locus_tag="PLG78_00465"
     CDS             complement(7000..7359)
                     /locus_tag="PLG78_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21605.1"
                     /translation="MNDQETAQRMIDAVKSVNLDAIEGARDAVNRSILEILLPEFEKL
                     TDDYQRAAFAMLICDLMDPITLPVMRKVLSLPDTEDIFNIARATALDHLAGKFGTAPD
                     WTRKEDLNRAVLEALWS"
     gene            complement(7383..8048)
                     /locus_tag="PLG78_00470"
     CDS             complement(7383..8048)
                     /locus_tag="PLG78_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21606.1"
                     /translation="MPKPEILRAKRAPASPRKAAVKSYHHGDLRSAVLDEADRLLEKH
                     GIENLSLRDVAQNLGVSHAAPYRHFPRKLDLLLALAEKGFSELGDAMEKAFTAKSPEE
                     RFQSSGLLYVKLAVAHPVRTQLMFSKTIQCDDAPDSLQQAGQRAFQGLVSIIEDGQKA
                     GEFKTNSDAMTLAVSAWSMVHGIAGLISGGQLAEAAGKEDGFVRTILSQLYSGVRKEK
                     RGR"
     gene            8190..9749
                     /locus_tag="PLG78_00475"
     CDS             8190..9749
                     /locus_tag="PLG78_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21607.1"
                     /translation="MRRIFFLLFTCLCLAQVSAEIHPPQSEKTPDKSGVTSKVTFSPD
                     LYSLEFIQQEDGSFKPSTENVTITFERKDPGTFCTKIKEVSASVWGPATEYVTVQDEQ
                     RGMWVANGNRMEFRGKTYKTSTGKSPGFCNFDGARIVIPAFEILSHMGTSSMNSSDRL
                     KSADVCGRYDENPVTHVPDFLFPRPVYIDTGFNSENAMDSYTRESRRILVSTRCRRAK
                     PEGAREVILRAAGKYLGVDPSRGFEMRSDRSSAGDTERFWLRSGASENWIRLQSTVGG
                     KYACQNDQNGQVYAAYNFDGKGKGCMDPRLEIVSPGRVRITGNVWVTTGSQTAAQKRY
                     WVPLDGLVKGSANATPETVFEILDANTRKPIAFSSAAGAKNSFSMGLADGSLPGAKLT
                     WKLVRVSDGMELNMPAPNQGSLIIEDFVGTEYRMDFFVSYQGKEYRPGDKPTVRQNEM
                     QPAVTGIVNANVQGSDEKPAAPPENRTFSLLEGDRKSHWYKVSFKEYLNVDTNKIRPN
                     KPDLLKPGPNR"
     gene            9867..10808
                     /locus_tag="PLG78_00480"
     CDS             9867..10808
                     /locus_tag="PLG78_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21608.1"
                     /translation="MKRFSDLLRMNPPLVLTVVANLGLFCVLLPLAFLDTRLVTGQPV
                     WIKPLKFAISVSLYSGTLMWLLGYVKKQVLARRVAWIVAVTLLIEMILIVLQAARGVA
                     SHFNVDAPVDGAIFSIMGLSIAILWIAHLVTSVSLLRDRTWTGPLSSAVRGGMIVAAL
                     GMVVAFVMTAPGFNGPVQFSPNGQPYPTGHIIGAPQDAPGLAILGWSTTGGDLRVSHF
                     VGLHALQILPLFALLLSAFKISAESRILLVRALSVVYGAVVVILLLQALRGIPFFRMD
                     SLTLGSYAVCAIFFAGVVLFVLFRGQSRGMDSKLIQE"
     gene            10813..11256
                     /locus_tag="PLG78_00485"
     CDS             10813..11256
                     /locus_tag="PLG78_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HNE21609.1"
                     /translation="MNASLIFQIANPAATIAWLVLVLFPGWKYVRVFTVTIMSSLVFA
                     GVYAFALGTSMAAGGSGNFSSLEGVSMLFQNPYALLAGWVHYLAFDLFVGSWISFHAQ
                     KLGISRWIILPSQFLTFMFGPIGLFTFLLIRALMRRESLATDPFA"
     gene            11339..12649
                     /locus_tag="PLG78_00490"
     CDS             11339..12649
                     /locus_tag="PLG78_00490"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNE21610.1"
                     /translation="MFIRLALILILLIAQACRRPIDFGPDNPGGLATVSATGESAFSL
                     PAANIDPRWIVKFARGNRSFKTPWSVQAGAEQPGLGPLFNHTSCAGCHPRDGRGQPPK
                     NPGDLDHGMLIQLGDSGKNHPLYGDTLQTRAIQGHNPEPSFRIRYEEIKGEYADHTPY
                     SLRKPIVEFNVQSETIPAFSARTAPALIGLGLLESISESEILIRSQRVKDVNPAIGGR
                     MNHVRNGDGDEIPGRFGWKASVARIEDQIARAAFRDMGVSSRLLPASLDSATAGNASE
                     PEMKDDVLANITFYIRSIGVPARRDPWNPDVQRGEEVFLRLGCANCHAETFVIGRTSL
                     DHLTYQVIHPYTDLLLHEMGPGLADHRSDGEATGSEWRTAPLWGIGLVQTVNGHEFFL
                     HDGRARGFEEAILWHGGQAEASRNGFLSLAARDRALLLQFLRSL"
     gene            complement(12652..15444)
                     /locus_tag="PLG78_00495"
     CDS             complement(12652..15444)
                     /locus_tag="PLG78_00495"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNE21611.1"
                     /translation="MRRHLATVFDRLFFVVGILILAVSAGLISVAQVLKRPWMYAWMV
                     SEDSLTVGRAETRTRQKFSEPHATYRWWQLRTTGKRYFQAERFADGLAHVQIDREMGY
                     IDTKGKPAFTARFNDGEQFHNGRAVVRVGYHYGVIDRQGEFVIAPVHFAISHLLTPDR
                     RLSFFSVSNSEYGAPQLLDANGKLLLDAAYRVTIGPRSLIAQGNKKVGADYEDFFAIY
                     DHAGRIISEQIGDFYNSRGIFVQNHLWGVMNDQGEITVAPSFNYLQFLQNGNIYGETE
                     CKPSLIGRDGTRTESMPLFKGTREENPNGHVYCEYMQDASRCPCVYSGGIITCNINDT
                     TKFRKPLNYIGPFSRGLAIAKRTANEPFGFVNESGEFVIAPQFDSAIEPDACFAYARK
                     DNAVFAIDPVTLQIRPALLSNPGRLQFKDGIACGEMESAQGQGLYANSNRTGCVNESG
                     TIQVPPRFWRIGEFSEGVAFAQTLGGNSGLIDINGKWISVLPALDIEPFSSDLAQFTD
                     STGYHGYIDRRGNVSLPPVYESAGNFNDGVAPVRRRSEKTTYIRKDGSRAFQNDFELG
                     YDFSEGLAAVSWSTNEAFIDRTGKVVFGGKYRYGSRFSEGLVSYSDSGSHLMGYVDRS
                     GNVQIPARYTAAYDFKEGLAAVKQACGTENCPFGYVDQSGQVRIAPHSNLTGAPFYNG
                     RATIQNDKMKFGLIDPSGAIIVSPIYDSLEPAGAGLYRARREGKAGFIDRNGKIISGK
                     FYQDARLFREGLAAVQSDSGFYDFIDPQGNKVIETGFSDVGNFSCGLALFGFTGSGYN
                     QKPPDAFERFYYPSKGSFFSKGEGKGQKADGGDYFRLDRYEAQVRYGFLNKNGEIQIP
                     PMFERAATFGIENEFEDRGEMDSETRKCISHVTAPDRKNAYYMDVHGDRIGDRPDYYP
                     YILYLLGQYQDWLTKP"
     gene            15457..15879
                     /gene="arsC"
                     /locus_tag="PLG78_00500"
     CDS             15457..15879
                     /gene="arsC"
                     /locus_tag="PLG78_00500"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258191240.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNE21612.1"
                     /translation="MAESKYPVNHSGFVIARLVRIISMHANMQIYHNPRCTKSRETLE
                     LIQDSGAKVEIVEYLKNPPTATELSAILDKLGMKAEDLIRKGETVYAEKYKGKTLTEK
                     QWIQAMVENPILIERPIVVSGKKAVLGRPPENVKKLLR"
     gene            15884..17386
                     /locus_tag="PLG78_00505"
     CDS             15884..17386
                     /locus_tag="PLG78_00505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21613.1"
                     /translation="MKNRYYLSMLGLFPFIIVIDGIYFVYHQSITIFLGLGFVHLLLY
                     GLINYIGARLLYRPVDRLFEGTGDLPAARKRIEQLTWQSTAWIFLIGNIYVAIALLPL
                     YFFPRIFQNPEDFVIDKIPPQFFFFSIVPGIYFVYALFPSFIAYFLINDFILDLKETV
                     SARFKMLFDAGSKGIGRTLFFVFLFLVFLPSLLVILELKVAFQVEDKYAQFTKLSPLQ
                     TVLIDRFIVFLGTIVAVVLVTRTFTKPIRSLIKAINRVREGDYSTQAAVITEDEIGLV
                     TQEFNGMVRGLQERELIRDTFGKYVTRDVATVILEEKIDLAGEVRTATILVTDIADYT
                     TISETLTPREIVMMLNEYFSELVEIIHAHRGVVNKFIGDAIFAMFNVPLDDPNHAANA
                     IRAALAIEKVTSSRTFGKDRKLRTRIGINTGVVVAGNIGSADRLEYTVIGDDVNIAAR
                     LEQLNKEHGTSVLLGENTYELAKDQFKFKELGGFQLKGKEKIIQVYRVSD"
     gene            complement(17368..>17660)
                     /locus_tag="PLG78_00510"
     CDS             complement(17368..>17660)
                     /locus_tag="PLG78_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HNE21614.1"
                     /translation="KLYLRMMPPWLPAQKAMNYISGAAEVFLGIGLVPLVTRAWAAWG
                     IIALLVAVFPANVYMLTSGKFKLPKPLLILRLPLQLVLIWWAYQYTGQSLTR"
BASE COUNT         4036 a         4409 c         4540 g         4675 t
ORIGIN      
        1 ctgtgccgct tccgtagggc tcatttcagt tttgagtttt ccgtctgtcc tggccttttc
       61 aagggcccgc gtgagcaggc tttccattgc atcgtagtgc ttgttgacgc acaccgcagt
      121 gacaggatct tccgggacca attctaacgc gctatttgcg atcatgcaac cctttctggc
      181 cgcttttccg tgagctgtgc cgactacaat gtccagggtt cggttcaacg cttccactgg
      241 tttgccggtt tccaggttct ttcgcattcc ttccaggagg ttttctgaat atgccttcaa
      301 ggatttgatg aagagagtgt gtttgtctcc aaacgctttg tagagactgc ccctcaggac
      361 tcccattgct tccgttagat cggtgatgga tgttgcatgg tagccttttt cccagaacaa
      421 attcacggaa gcctgcaggg catcgtcact gtcgaattcg cgcggtcggg ccatgtggtg
      481 agtacgcgaa aatgacgttt cgcagtccat aaatttttta taacttcgca cgactttcgc
      541 ctacgcttcg cacgctctat ttcgcgcgcg aaagtgctct cgcgacgagt ccgaagactg
      601 tgcgaaatgg gattagccca tacgaaaaca cgatcgacgc gacgcgagcc agtggtggca
      661 ctttcacgtt ctggagtttg tccggattca ggatgctgta gacagcgtcg atctgcccgt
      721 tgcggtacga gagattgagg atcgtgtaga ctttgccgcc tgtgagcaaa acgagtgcag
      781 gctctcctcc tccttctacg agcacaggcc aggcgtcagg cggttgtttt tttgcaacac
      841 cgagaatgcc gcgaatgcat ttggcggcac cactgaccac gtttcgcgcc gcgttcacct
      901 taccgccgcc atctgaaatc attctcgcgt cttcgcacag cagcttaccc agagaatgga
      961 catctccttt ctgaacggcg cgaaggaacg cgcgaaaaag ctgatctttt ttctgaggag
     1021 gagtttcaaa ccgaatggct tccgaggaaa cgtgtttgcg cgcacgcgaa gagatttgtc
     1081 tgcaattttc ttctgatttc tcgagcatcg aggcgatttc tgcgtagtcc acatcgaata
     1141 cgtctcgaag tagaaacaca gcgcgttcca caggactgag cctttcgagc ataagcaaga
     1201 aggccgtgga tagactctcc attcgcacgt aatcttcttc gggactttcc gtgattggtt
     1261 cgggaagcca gggcccaatg tatgtgcgac gcctttcata ggcagaatcc agaaaatcga
     1321 tgcacatcct ggtgatgaga gtgatgagga aggcccgcga gttatttact gcattgagcg
     1381 ctacgttctg aaatttcaga tagccttcct ggagaatatc ctgcgcatca gacgagctcc
     1441 ccgtcatcct gtaagcgatg gagaaaagaa gccgtctgtg ttcggtgtat agacttgtgt
     1501 gggtcatgac tttgcgcgaa tgcgagccat tgctttttct gcggccgtta ctgcagtgaa
     1561 agcacttcga tcggagagca tgcctgatcc gcgcacccag tccccggcgc gaaacagtgg
     1621 tttggcggag tcaatttcta ccccgggctg ttcggttgta tcctggaaat cgtgtgccac
     1681 aatcatgtcc ggtaaaaatc gttcctgtac gattcgatct tcgctgcctg gctggaacct
     1741 ggcaagaaaa tcgcgtaact gcttcttcgt cccttcgccc gcttcttctc cttctttgag
     1801 atagcgcgct acatggatga cctgacccgg gccaagattg gcgctggcgg aatggaccga
     1861 tagataaaga ggttcagcaa tatcctgtgc gaagatatga tccggctccg ggaggcggtc
     1921 aagtgccacg tcgagacacg cagcgcgaag tttcttgcca cttccaatct ttgttccaag
     1981 ccaggcgttc tccgattcag gatcggcggc caggatagcg gtcctgcttt ctatgatgcg
     2041 gtctgttgtg tgtaagcgca cttcatctct gctggattct atttgttcca ctttctggcc
     2101 tgtcaggaat tccacgccca gagcacgcgc agagttttca agaccgtcta cgattgtttg
     2161 ccagcctccg tccagataga gtacgccgcc cgtggccagt ttcaattgat ccgcggccgc
     2221 atgtgcaacg attctttccg gctctgcatt gtacgttgac agtctcacga gagaagaggc
     2281 gaatcccttt gcctttgcgt ctttgatttg atccagttca aacgcaagtg cgcttccatt
     2341 cattctaccc atgattgctc ttccatagaa tcgaatcatg tccactcttc cttttctgga
     2401 aaaccatgga tgagtgagaa gtccaagtac gtttgttggc atagaggcga attcgctttc
     2461 gcgaccgatg tacgttccgc gcggtgctcc ttttttccct gtcactttga ttcccagatc
     2521 agacagagtg cgcgccagat ttccgcgaat gtagagagcg tgcggtccct gattgaatat
     2581 gaattggtcg cgcaccgcac tcctggctct tcctcccagt gattttgatt tttcaaccac
     2641 cgtcacgctg agtcctgctc tggctgcgat atttgcggcg atcaggcccg cgaggcctcc
     2701 gcctattact attgaatctt tcattgtttc ctcccgcggt cgaaccgctt caaaggggag
     2761 acggggggca ggtgtggttt gtgacagagg gtctgaattt ttttacgtac aggaaacgcg
     2821 cctaaagatg atgggtttgt ggatcacata gacgtcatcg tcccagataa ctgtgatcgt
     2881 acccgccttc ttgtccagtc tgcccttgaa tcgatccatt tcatagttcc agtctggctg
     2941 tgttggatcc atgcatatac gttcggtccc tgttagttcg attgtgcgaa agaagaaatc
     3001 attgccgctt aacgttccat cccccggcag cttgaagcga atgatatgat ccggattctc
     3061 gcagtgtgaa ggcggttggg atgggttcag attgggcgaa tagcttagat tcaagagcag
     3121 gccgcgcaat ttacccgctt tgttttcgtc gatgcgaagg gtgaggtgct gcttgcgttc
     3181 tccgtgcgtt gtcagtgcga tccaggtccc gacaaccgga tcttctttgc acttgtcctg
     3241 acccacaact gtgggcagga cacgcaccgg gaacaaaaat agaactacgg gcagaacaaa
     3301 taggaccgag aatgccgtcc gggcattact cggtcgggct cggttgttgt ttgagcgcat
     3361 tgaaataact cagcgcgcgc agcatcatat aatctggttt gagcgcatcg cctttgtgca
     3421 gtttgatttc ttgttctgcc ttctcgttgt ttccaaccgc ggctttgatc ttctgaatcc
     3481 aggcttctcg cgcaggattg cggtcgcgcg ccgtgagttc aggaagatgc ttccacatat
     3541 cttcttcgcg gaaacgtggt ggaaacccgt tgtcctcttc ttctgagact gcgatgtctg
     3601 gctgcacccc gtacacctgg atcgtgtaac cattcggagc atagtagcga gcagtcgtga
     3661 gtttgatcag gatcttgccg cggggttgca gactctgtac ggttgctttt ccgaaacttc
     3721 tttctccgag cacaagagct gtgttgtggt ccatgagagc ggatgccaca atctcagaag
     3781 cagaggctga gcctgcattg atcagcacga tcatgggaag gtcagccggg atcatgcgag
     3841 actcatctgc gcgtctttct tctttgtctc cgcgcctttt gatctgcacc accactctgt
     3901 tttccggaac aaagagtccg gcgattcgaa tcgcttcatc cagatctccg cctggatttc
     3961 cacgcagatc aagaaccagt ccgcccagtc tgccgcctgc ttttttcaca agtgattcgt
     4021 attcatggcg aatcagttct gtaggacgtt tctcagagaa aagaaatgag ctgagcttga
     4081 tgaccccaat ctttccatct tctatataag aagagcttac tgatttttgc acgatcactc
     4141 cgcgcttgat gcgaatgatc agattggcca cttccgtctt gcgttcaatt tcaagagtga
     4201 cgaaagtctc ttttggtccg cggattcgtt tgactacttc tccaaggacc atgttctcga
     4261 tggagttgcc gtctactttt acgattacgt ctccggagcg aatgcctgcg ttgtgagcgg
     4321 gtgatcccgg cagaggagtc tctactacta cttcctgatt tccgccgccg cgaagcatgg
     4381 cgccaattcc ttcaaaggaa gaatcttcag actcttttag aatcttctgc catgattcca
     4441 tatccatcac agcactgtgc ggatccatgg tttgcagaaa tccattggca gatgcgaaaa
     4501 acacatgctg gattccaaaa ggttcatcgc ggtaaggatt ttctcccttg tagctgggag
     4561 gaagagtgct gtactggcct ttgttctttt cgatccaggc aatcactgtt tcgaaatctt
     4621 ttctaccaaa tccaacatcg ttccaggctt tctccagagt ttcctgttct gccatgattt
     4681 ctttgcgttg tttttccact tcttcctggc gttgtttgga agagatcttc ttgcggcgct
     4741 ccctttcctt tgcagcgagc gcatcctgtt tcttatccca ggcagaatag tcgggggcca
     4801 gaacaacgta agcggctcct ttttctattt caacaatgtt gccaggaatc tttttgtcct
     4861 ccggctgctt tgctcggagg tcctggtagt atttgcgtgg ataaaggatg agaggattgg
     4921 gcagagaacg aagtgccgat tctgcagcac ccacatacgc gcggttgtct ttaatattgt
     4981 ccggatcaat gtagttctgg cgcgcgaagc gcagtatctc atcgaattga gtccaggaga
     5041 actctcctgg aacgcggctc tgcgccggat tgcagccgat actatagaca aaggcggtca
     5101 gtatggcaga cagcgcaaag gactggaagg gtttcacgaa aaataacctc agggtataca
     5161 cggttttggg atcgttcatg cagggtggtt ctcttgcgtc gattcttttc attcttttct
     5221 gtggatgcag taccgcgcct gttcaggatc ggccagtcca tgatcgttta gacgcatacg
     5281 agctcgcatt tcacctatat tttcgagaag aaaggaaaga aaacgcaata gatttgctgt
     5341 cctgggcttg caggagcctg gaggatgggc gctatatcag ctgttacgac ctgggccagc
     5401 tcttgctccg agaaggcaga aaagacgatg cggcgcgcgc atttgcggaa tcgtacagaa
     5461 tttcgccgaa cggacccgct ctgtctgccc tctacgatct ctgggcgacc ggtacaggtc
     5521 caactccgcc tccagctccc gcactcgttg tgatttccaa gacagtggag cgattttgcc
     5581 gggccgggga tagacaggca gccatgatcg cccttaaatc ctccccggcg gacataccca
     5641 gggagctttt cgcacagcca ttttttcagg agtgcttgtt caaccagcca gagtatgctc
     5701 ctttcatcgc tggtcgaact ccacggccag tgccgatcga tgagatctat cgccagagag
     5761 cggctgttca tcctctacat gcagtgctcg atgttgctcc ttatctgaaa gcggaatatc
     5821 ttgcgtttga atcgaaccat ccagtgaacg tttcgtggaa attgtttttg aatgcggtgc
     5881 gcgcaagaaa ccaggttgcg gcggaaagcg cgctggcgaa cttttatgct gcgctcgaaa
     5941 aacagcgggt gccaggcggc gccgccgatt cacaaattct cgccatgaag cgtgcggccg
     6001 gaactttaat agcaggagac attttttttg cgtctattcg cggttcagcg cggattcaga
     6061 aaatggtgag ggatactctg ccaggctccg gaatcttcgc gagcaagtga attttcacca
     6121 tgcgatgatc cgatcatgga gtagatgaag cgcccccata atcgcatcga gaaaatcacg
     6181 aacaatccca aagtagtgag tcttgctctg tacaggatca agaaggggct gaagaaagaa
     6241 ggctttgagc tgatcacaga cgagcaggga aaattgaagc tcgtcatgcg taagaataag
     6301 agctaaaaaa cagttgcccg gcgtaaatcg agtcctaatt tgattggata acggctcgaa
     6361 cgtccggagg aactgtgaaa atcgaaaaac ataaagtcgt cagcattgac tacacgctga
     6421 aagacgatca aggcaacatc attgattcat cgcgcggagg agaacctctc gcatacatcc
     6481 agggagtggg caatttgatt cctgggcttg aatccgctct cgagggcgga tcaaagggag
     6541 atcgtctcag tgttagcgtt cctccggaaa gaggatatgg agtcctcgaa gatcacctaa
     6601 ttcatgttct gcgacgcgat caatttgaag gcgtagaaga gctagaagtt ggaatgcagt
     6661 ttcacgcgca tgcggacaac ggcgcggttc agacagttgt agtcacaaag atagacgggg
     6721 acaatgtaac gatagacggc aatcatccac ttgctggaaa gaatcttcat tttgatgtgg
     6781 ctgtcgtgga agtgcgggat gctactgcag aagaactcga acacgggcac gttcacggtc
     6841 caggcgggca ccatcactag aagctagcgt gcttgaatga atccgcgaga gcggattcag
     6901 gtctgcttgc aagaagtagc gtggtactgc gcaatcacat cggttctgtc atccagaatc
     6961 cgcaggaggg tacgttatac gatttttctc aaccttccgt taggaccaga gcgcttccag
     7021 aactgccctg ttcagatctt ccttgcgtgt ccaatcgggc gcggtaccaa atttcccggc
     7081 taaatggtcc agcgctgtcg cccgcgcaat gttgaaaatg tcctcggtat ccggcagact
     7141 cagtactttg cgcatcacgg gtagagtgat cggatccatc aggtcacaaa ttagcatagc
     7201 aaaggccgcg cgctggtagt cgtccgtgag cttctcgaat tcaggcagca atatttcgag
     7261 gatgcttctg ttcaccgcat cccgcgcgcc ctctatcgca tccagattca cgcttttcac
     7321 agcatcgatc attctttgag cagtttcctg gtcgttcata tcgcgtattt cgtataaccg
     7381 tctcagcgcc ctctcttttc ttttcgcacg cctgaataaa gctgcgatag aatggttcgc
     7441 acaaatccgt cctctttgcc cgccgcctcc gccagctgtc cgccggaaat caaaccggcg
     7501 attccgtgaa ccatgctcca cgcggaaaca gccagagtca tcgcatcaga atttgttttg
     7561 aactctccgg ctttttgtcc gtcctctatg atcgaaacca gtccctggaa cgctctctgg
     7621 ccggcttgct gcaaagaatc gggagcatca tcgcactgaa ttgtttttga aaacatcaac
     7681 tgggttcgca caggatgagc gactgcaagc ttcacgtaca gcagccccga cgattgaaag
     7741 cgttcttctg gactttttgc cgtgaacgct ttttccattg cgtcgccgag ttcactgaat
     7801 cccttttctg cgagagcaag aagtagatcg agtttgcgtg ggaaatgtct atagggtgcc
     7861 gcatgactca ctccgagatt ctgagcaacg tcgcgaaggc tcagattctc gattccgtgt
     7921 ttctcaagca atctgtctgc ctcgtccagt actgccgagc ggaggtcgcc atgatgatac
     7981 gattttactg ctgctttgcg ggggctggca ggagcgcgtt tcgcgcgcaa aatttccggt
     8041 ttcggcatgt atacagtgtc tacattttcc gaatgtacgt caagcagaac gcggccgctt
     8101 cgcccgcctg cgtcgcactc cgggcgatat ccaggttcta aaattttttt aatattgatc
     8161 aaatcgctac cgtactgcat ctaaaccgca tgcgaagaat atttttcctt ctgtttacat
     8221 gcttgtgcct ggcgcaagtt tctgcggaga tccatcctcc ccagagcgag aagactcccg
     8281 ataagagcgg agtaacttca aaagttacct tttcgcctga tctttattct ctggaattca
     8341 tccagcaaga ggacggttcg ttcaaacctt ctacagagaa tgtaaccatc acttttgagc
     8401 gcaaagatcc cggaacgttt tgtacaaaga taaaggaagt cagcgcatcc gtttggggtc
     8461 cggccacgga atacgtcact gttcaggacg aacagcgggg catgtgggtt gccaatggaa
     8521 atcgaatgga gttcagaggc aagacataca aaacatccac tggcaagtca ccagggttct
     8581 gcaattttga cggcgccaga atagtcattc ccgcgtttga aattctcagc cacatgggaa
     8641 caagttccat gaattcgtcc gatcgtttga agagtgcgga tgtgtgcggt agatacgacg
     8701 aaaatccagt cacgcatgtg ccggatttct tgttcccgcg acctgtctac attgacacag
     8761 gattcaactc agaaaacgcg atggattctt atacccgcga gtcacggcga attctggtgt
     8821 ccactcggtg cagacgtgcg aagcctgaag gtgcgcgtga agtgattctg cgcgccgcag
     8881 gaaagtacct gggcgtggac ccttcgcgcg gttttgaaat gcgttctgat cgttcgagtg
     8941 caggcgacac agagcgtttc tggctgcgaa gcggtgcttc cgaaaattgg attcgcctgc
     9001 agtccaccgt aggcggaaag tatgcatgcc aaaatgacca gaatggtcag gtgtatgcag
     9061 cgtacaattt tgacgggaaa ggcaagggtt gcatggaccc gcgacttgaa attgtaagcc
     9121 ctggtagagt gcgaatcacc ggaaatgtat gggtgaccac cggaagtcaa accgccgcac
     9181 agaaacgata ctgggtgccg ttggacggcc ttgtgaaggg gtctgcgaac gcaactccag
     9241 agacagtctt tgaaatcctg gatgcgaata cgcgcaaacc aatcgctttt tcgagcgctg
     9301 cgggtgcgaa gaattctttt tcaatgggcc ttgcggacgg ttctctgcct ggagcgaagc
     9361 tcacctggaa attggttcgc gtttcagatg gaatggaatt gaacatgccc gctcccaacc
     9421 agggaagtct tattatagag gatttcgttg gaacggagta tcggatggac ttctttgtct
     9481 cttaccaggg caaagagtac agacctggag acaagcccac cgtccgccag aatgaaatgc
     9541 agcccgcggt tacagggatc gtgaatgcaa atgttcaggg cagtgatgag aagccagccg
     9601 caccgcccga aaacaggacc ttctccctcc tcgagggaga ccgtaaatca cactggtaca
     9661 aggtttcttt caaggaatat ttgaatgtgg acacgaacaa gattcggccg aacaaacccg
     9721 atctattgaa accgggtcca aatcgttagt ttcgcaagtc tgtggaatgt agacactgac
     9781 tacttttttt gttgacccgc ccccggggta tgtatacagt gtctacattc aataacggct
     9841 gctcaatttt gctgccggga gactatatga aaagattttc tgatctattg cgaatgaatc
     9901 cgcctctcgt tcttacggtc gtggccaatc ttggtttgtt ctgtgtgctt ctacctctcg
     9961 cattcctgga caccaggttg gtcacggggc agccggtgtg gatcaagcca ctgaagttcg
    10021 ctatttctgt ttcactctat tcaggaacgc ttatgtggct tcttggatat gtgaagaagc
    10081 aagtactggc cagacgcgtt gcctggattg tagctgtgac tcttttgatc gagatgatcc
    10141 tgattgtttt gcaggctgcg cgaggagtgg ccagtcactt caatgtggat gctcctgtgg
    10201 acggagcgat tttctctatc atgggtctca gcattgccat cctctggatc gcgcatttag
    10261 tcacttcggt ttcgcttctt cgtgatcgca cgtggactgg gccgctcagc agtgcggtgc
    10321 gcggaggaat gattgtggcg gcccttggaa tggtcgtggc ttttgttatg actgctccgg
    10381 ggttcaacgg gcctgtccag ttcagtccga atggccagcc gtatccaacc ggacacatca
    10441 ttggcgcgcc tcaagacgca ccaggtcttg caattctagg gtggagcacc accggcggag
    10501 atttgcgggt cagtcatttt gttggtcttc acgcgcttca aattttaccg ttattcgctc
    10561 ttcttctgag cgcatttaag atatccgcgg aaagtcgcat tcttctggtt cgggctttga
    10621 gcgtcgtgta cggcgcagta gtagtgattc tgctcttaca agcactgcgc ggcattccat
    10681 tttttagaat ggattctttg acactgggtt cctacgctgt ctgtgcgatc ttctttgctg
    10741 gagtagtact gtttgttttg tttcgcgggc aatcgcgagg catggattct aaattgattc
    10801 aggagtaaaa aaatgaatgc atctctaatc tttcaaattg caaatccggc cgcgacaatt
    10861 gcgtggcttg tcctggtgct gtttccaggg tggaaatacg ttcgtgtttt cacggtgacc
    10921 atcatgagct ctctagtatt tgcaggcgtc tatgcattcg cgctgggaac gagcatggct
    10981 gcgggcggaa gcggaaattt cagcagcctc gaaggagtgt ccatgctgtt tcagaatccg
    11041 tacgcacttc tggccggctg ggtgcactat cttgcgtttg atttgtttgt aggaagctgg
    11101 atttcttttc atgcgcaaaa gctgggtatt tctagatgga tcattcttcc gtctcagttt
    11161 ctgacgttca tgtttgggcc gatcggtctg tttactttct tattgatccg cgcacttatg
    11221 aggcgcgaat ccctggccac ggatccgttc gcttgagaca aatcagatcg gcgcgctctt
    11281 gagatgagcg cgcccccttt gtgattgcgt cctgggcgga actttcctaa tcaggatcgt
    11341 gttcatccga cttgcgctga ttctgattct gctcatcgct caagcatgca ggcgccccat
    11401 tgattttggc ccggataacc cgggcggact ggccaccgtc tcggccactg gagaaagcgc
    11461 attttctttg cccgccgcca acatcgatcc acgatggatt gtaaagtttg cccggggaaa
    11521 ccggagtttt aagactccat ggtcggtcca ggctggcgcc gagcaaccag gcctcggacc
    11581 tttgttcaac catacttcat gcgccggatg tcatccgcga gatggtcgtg gtcaacctcc
    11641 gaaaaatccg ggtgacctgg atcacggaat gttgattcaa ctgggagatt ccggaaagaa
    11701 ccatccgtta tacggcgaca ctctccagac tcgtgcgatc cagggtcata atcctgaacc
    11761 gtcttttaga attcgctacg aggaaatcaa aggagaatat gcagatcaca ctccgtattc
    11821 gcttcgtaag ccgatcgttg agttcaatgt gcagtcagaa acgattcctg ctttttcagc
    11881 gcgcacagct cccgctttga tcggactggg tctactggaa tccatcagcg agtctgaaat
    11941 cttgattcgt tctcagagag tgaaggatgt aaatccggcg atcggcggca gaatgaatca
    12001 tgtgcgcaac ggtgacggag acgaaattcc aggtcgtttt gggtggaagg caagtgtggc
    12061 gagaattgag gatcagattg cccgcgcggc ctttcgtgac atgggtgtat catcccggct
    12121 gttgcctgct tccctggatt ctgcgactgc cgggaatgcg tccgagcccg agatgaagga
    12181 tgatgtactt gcgaatatta cgttttacat acgatctatc ggagtgcctg cgcgaagaga
    12241 cccctggaat cctgacgtgc aacgaggcga ggaagtgttt cttcgactgg gctgtgcgaa
    12301 ttgccatgcg gaaacatttg tcattggtag aacttcactt gatcatctaa cctatcaggt
    12361 gatccacccg tacacggatt tgttgctgca tgaaatgggc ccggggcttg cggatcatcg
    12421 cagcgatgga gaagcaacgg gtagcgaatg gagaacggct cctctctggg gaattggtct
    12481 ggtgcagacg gtcaatggcc atgaattttt tcttcatgat ggtcgtgcgc gtggctttga
    12541 agaagcgatc ctgtggcacg gcggtcaggc ggaagcgtct cgaaacggtt ttctttcttt
    12601 ggccgcgcgc gatcgagcac ttctcctgca attcttgcgc agtctctagg tctaaggttt
    12661 tgtcagccag tcctgatact ggcccagcaa gtagagaatg taagggtagt agtcaggtcg
    12721 atccccgatc cgatctccgt gcacatccat gtagtacgca ttttttctgt caggcgcagt
    12781 cacgtgagag atgcattttc tggtttctga atccatttcc cctctgtctt cgaattcgtt
    12841 ttctatgccg aaagtggctg cgcgttcgaa cataggcggg atctggattt ctccattctt
    12901 attcaggaag ccataccgca cttgtgcttc atatcgatcg aggcgaaagt aatcacctcc
    12961 atctgccttc tgccctttgc cttctccttt tgagaagaat gatcctttgc tcgggtagta
    13021 aaagcgttcg aacgcatccg gcggcttctg attgtagccg gatccggtga acccaaacag
    13081 ggcaagtccg cacgaaaaat ttccaacatc tgagaagcct gtttcaatga ctttgtttcc
    13141 ctgtggatct atgaaatcgt aaaagccaga gtctgattgc actgctgcca gaccttctct
    13201 gaacagacgc gcatcctgat aaaattttcc agaaatgatt ttcccattcc tgtcgatgaa
    13261 tcccgctttt ccttcgcgcc ttgccctgta caatccagca cccgccggct ccagtgagtc
    13321 ataaatcgga gatacaatga tcgcgcccga tggatcgatc agtccgaatt tcattttatc
    13381 attttgaatg gtcgcccttc cgttataaaa gggagcacct gttagattgg aatggggtgc
    13441 gattcgaacc tgaccactct gatcgacgta gccaaaaggg caattttcag ttccacaggc
    13501 ttgcttcaca gcggcgagtc cctccttgaa atcatatgcc gcagtgtaac gtgcgggaat
    13561 ctgtacgttt ccggatcgat ccacgtatcc catcaaatgt gagccagaat cagaataact
    13621 tacaagtcct tccgaaaagc gagacccata gcgatatttt ccaccgaata cgactttgcc
    13681 tgtgcgatcg atgaaggctt catttgtaga ccatgaaact gcggcgagac cttcgctaaa
    13741 gtcataaccc agttcgaaat cgttctggaa tgcgcgactt ccgtctttgc gtatgtaggt
    13801 ggtcttttct gatctgcggc gaaccggtgc gactccgtcg ttgaaattcc cggcgctttc
    13861 atacacagga gggaggctca cgtttcccct gcgatcgata tatccgtgat atccggtgga
    13921 atcagtaaac tgcgcaagat ccgatgaaaa tggttctata tccagcgcag gtaagacaga
    13981 aatccatttt ccgtttatgt cgatcaatcc tgaattgccg cctaacgtct gcgcgaaggc
    14041 tacgccttcg ctaaattcgc cgattctcca gaagcgaggc ggcacttgaa tggtgcctga
    14101 ttcatttacg caaccggttc gattggagtt ggcatagaga ccctgtcctt gagccgattc
    14161 catttcgcca catgcaattc cgtctttgaa ttggagtcgt ccgggattgg atagaagtgc
    14221 agggcgaatt tgtagtgtta caggatctat cgcgaagacc gcgttgtctt tgcgtgcgta
    14281 ggcaaagcag gcgtctggtt ctatggcaga gtcgaattgg ggagcgatta caaattcacc
    14341 tgactcgttc acgaatccga aaggttcgtt tgccgtgcgt ttcgcgatgg cgagccctcg
    14401 cgaaaatggt ccaatgtagt tcaaaggttt ccgaaacttc gtagtatcgt tgatattaca
    14461 ggtaatgatt ccgcctgaat aaacacacgg gcatctggat gcatcctgca tgtattcgca
    14521 gtacacatgc ccatttggat tttcttctcg cgttcctttg aatagcggca tggattcggt
    14581 tcgcgttcca tcccttccaa ttagtgaggg tttacactca gtctctccat agatatttcc
    14641 gttttggaga aattgcagat agttgaatga aggggctact gtgatctcgc cttgatcgtt
    14701 catgactccc cataagtggt tttggacgaa aattccgcgg ctgttgtaaa aatctccgat
    14761 ttgttccgaa atgattctgc ccgcgtgatc atatatggca aagaagtcct catagtctgc
    14821 acctaccttc ttgtttccct gtgcgatcaa agagcgaggt ccaattgtga cacgatacgc
    14881 ggcatccagg agtaatttgc catttgcgtc taacagctgc ggggcaccgt actcggagtt
    14941 ggagacagag aagaaggaga gacgacggtc gggcgtgaga agatgagaaa tggcaaagtg
    15001 caccggcgca atgacgaatt cgccctgtct atcaatgacg ccgtagtggt aaccgactcg
    15061 gaccacagcc cttccattgt ggaactgctc tccgtcgttg aagcgcgcgg taaatgcagg
    15121 ctttcccttc gtgtcaatgt agcccatctc acggtctatt tgcacatgcg ccaggccatc
    15181 tgcaaaacgt tccgcctgaa aatagcgttt ccccgttgtg cgcaattgcc accaccgata
    15241 ggtggcgtga ggttctgaaa acttttgacg tgttcttgtt tctgctctac ccactgtgag
    15301 cgagtcttcc gaaaccatcc aggcatacat ccagggtcgt tttaaaacct gtgcgacgga
    15361 aatcagccct gcgctgactg caagaatcag aataccaaca acaaagaata gccggtcgaa
    15421 tacggttgca aggtgtcggc gcaaagtagt gcatctttgg ctgagtcaaa atatccagtc
    15481 aatcattctg gctttgtgat tgcacggctc gttcgcatta tttccatgca tgcaaatatg
    15541 cagatctatc acaaccccag gtgcacaaaa agtcgcgaaa ccctggaact cattcaggac
    15601 tccggcgcaa aagtagaaat agttgaatat ctgaagaatc cgcccactgc caccgagctg
    15661 tctgctattc tcgacaaact tggaatgaaa gcagaggatc tgattcgcaa aggggaaact
    15721 gtttacgcag agaaatacaa aggcaaaact cttacggaaa aacagtggat ccaggcaatg
    15781 gtggaaaacc cgattctaat cgagcgtccg attgtagtgt ctggaaaaaa agcagtcctt
    15841 ggaaggccgc cggagaatgt gaaaaagctt ctgcgatgat cgaatgaaaa atcgctacta
    15901 tctttcgatg ctgggtctct ttccgtttat catcgtgata gacggaatct attttgtcta
    15961 tcatcaatcc attacgattt ttctcggact tggctttgtg catttgcttt tgtacgggct
    16021 tattaattac atcggcgcgc gtctactcta cagacctgtg gatcgtttgt ttgagggaac
    16081 tggagatctg cccgcagcca gaaagagaat cgaacaactg acctggcaat ctactgcgtg
    16141 gatttttctg attggaaaca tttatgtggc gattgctctt ttgcctctct acttttttcc
    16201 gcgtatcttt caaaatccgg aagactttgt catagataaa atcccgccgc agtttttctt
    16261 tttctccatt gtccctggaa tttattttgt gtacgctctc tttccgtcgt tcattgcgta
    16321 tttcttgatc aacgatttca ttctcgattt aaaagagact gtaagcgcgc gctttaaaat
    16381 gctctttgat gccgggagca aagggattgg acggactttg tttttcgttt ttctgtttct
    16441 tgtgtttctt ccatccttac tggtcatact cgaactcaag gttgcgtttc aggtcgagga
    16501 taagtacgcc cagtttacaa agctcagccc tctacaaaca gttctgatcg atcgcttcat
    16561 tgtatttctg ggaaccatag ttgctgtagt gcttgttaca cgcaccttca ccaaaccaat
    16621 ccgatccctc atcaaagcaa tcaaccgggt acgcgaagga gactattcta ctcaggccgc
    16681 cgtcatcacg gaggacgaaa ttggccttgt aacccaggaa ttcaacggga tggttcgagg
    16741 cctgcaggag cgtgagctca ttcgagatac attcggaaaa tatgtgactc gagatgttgc
    16801 gaccgtgatc cttgaggaaa aaattgatct ggcaggtgaa gtgcgcacgg ccacaattct
    16861 ggtgaccgat attgcagact ataccacgat ttcggaaacg ctcactccgc gcgaaatcgt
    16921 aatgatgctc aacgaatact tttctgaact tgtagaaatc atacacgctc acaggggagt
    16981 ggtcaataag ttcatcgggg atgccatctt tgccatgttc aatgtgccac tggatgatcc
    17041 caatcacgca gccaatgcga ttcgcgcagc tcttgccatt gagaaggtaa cgtccagtcg
    17101 cacgtttggc aaagacagaa agctcagaac tagaattgga atcaatacag gagtggttgt
    17161 agccggcaac attggatcgg cggatcgcct cgaatacaca gtcatcgggg atgacgtcaa
    17221 tatagcagcc agactggagc aactgaacaa ggaacacggc acttccgttc tactcggaga
    17281 gaacacatac gaactggcaa aagatcagtt caaattcaaa gagctgggtg gcttccagct
    17341 caagggaaaa gaaaagatca ttcaggtcta tcgcgtaagc gattgaccgg tatactgata
    17401 ggcccaccag atgagaacaa gctggagtgg gagtcgcagg atcagaagcg gctttggcaa
    17461 tttgaacttt ccgcttgtga gcatgtagac gttagccgga aacaccgcaa ccagaagtgc
    17521 aatgattccc caggcggccc atgccctcgt tacaagtggt accaggccaa ttcccagaaa
    17581 aacttctgcc gcgccgctga tgtaattcat agccttttgc gctggaagcc agggaggcat
    17641 cattcttaaa taaagtttag
//
LOCUS       DAOQJL010000006        17447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1538_length_17447_cov_4.918066, whole genome shotgun sequence.
ACCESSION   DAOQJL010000006 DAOQJL010000000
VERSION     DAOQJL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1538_length_17447_cov_4.918066"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLG78_00515"
     CDS             <1..191
                     /locus_tag="PLG78_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21615.1"
                     /translation="SKADGLIPWKHSEELHALYNGPKKLWITETGEHAAVWNTHKEEY
                     ERALTDFLNQPPIIAPRR"
     gene            complement(172..3591)
                     /locus_tag="PLG78_00520"
     CDS             complement(172..3591)
                     /locus_tag="PLG78_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE21616.1"
                     /translation="MDKIVVQVPSDLSELIPGYLKNLSKYADQLKELISAGDLAAAGR
                     IGHNLKGSGGGYGLDEISTIGKLIEEKAHASDASAVKEALQKLNDYLSRLHVENGLSS
                     PRFHADLSISTMGKFAKSCGLVCLCLFSSCGQYDAVTGYFAVTFLNPAFFRSGGTPGF
                     TIRQSTPCITSEGGLNHTFTVETNTTPGALVTVPVSSLLTTEGVVSPSSVSLSVAGGT
                     QTITVSGVDDGSVDGNQSYTIALGNASSYDPSYNNLSVGSVSCVNLDDEQRRVSAWPL
                     TGLTVAESGTTATFSVLLSSAPASNVIVDVTSLDTTEGTVSPASLTFTPANWSTAQTV
                     TVTGVNDFLQDGSIAYNVALGINAASNAAWVATGTTDTVAVTTTDDDTAGVTVTPTSL
                     STVESGVAKTFSVVLTSQPTSSVTISAASADTTEGTVAPPSRTFTAGNWNVPQSFTVT
                     PQADAILDGTITYNVTLGPVVSADANYSGMIVSPVSVANADAGIPDIVVSPTSGLVTG
                     EGGVNATFTVVLTQQPSSNVVVGISSSDTTEGTVAPATLTFTNANFATPQTVTVFGVN
                     DAILDGNIAYTIVTAPAVSADPNYSGLNPPDVSVTNNDNDQNLVNISSSTNNLYTTEG
                     GGQVILKVVLSAQPLQDVDVGPITVDDGTEASVSPGTLTFTSANWNVAQDVTVTGLPD
                     AVVDGTIIYHVDLGTTTSTDLNYNGITPTDYNGGAAGQVTIRNADINGATKIVRCDSN
                     SLTTTEAGGTALMFYTLTQAPSANVDIPFSSTDTTEFTVSPATLTFTPANWNTCRQVT
                     LTGVDDAVDDGNQTRTLTIGAATSGDAAFNGIDLADPNVTNNDNDTAGYTVSAISGNT
                     TEAGGTATFTIRLNSKPTNNVTVNLSSSNVAEGTVSPASVTFDATVGACMGGGDWCTN
                     QTVTVTGVNDTAKDGNIGYSIITAQSTADPVYSLINPADVAVTNVDNEKYIFITASTY
                     DGAFDFNASLAGGANSAVNGDGNPRQEMDNACQTDANKPASGTFRALAVDASHRRASL
                     TANLGDSQISWVLSPLYDYIRYPGGATLFTSDANSIFVFGSLTNNFGAGSTYWTGLNG
                     DWTTGSHCTSWTDNGIAVSGSVGDGSSTGSNAISITTNTCDTLKPLICVEQ"
     gene            complement(3609..6047)
                     /locus_tag="PLG78_00525"
     CDS             complement(3609..6047)
                     /locus_tag="PLG78_00525"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNE21617.1"
                     /translation="MEPVSQSHWEDLGNSIKRHIVFVLGNDFQPPRKDTYYSGLAYCV
                     RDRLIERWLSGQRLAYETHPKRVYYMSMEFLPGRFLKNFIINLGMEAESREALKESGF
                     ALEELEEQEPDPGLGNGGLGRLASCYLDSMACLNIAAHGYGIRYDYGIFHQKIVNGWQ
                     VEQCDNWLQFGNPWEITRRHFLYTVKLFGRSEPYHDERGNLRYRWVDTDTVDAMACDI
                     PIPGYNNETVNNMRLWAAVSSRDFNLDFFNHGDYVRAMEEKVLSENISKVLYPSDEKE
                     QGRELRLKQQYFFVAATFEDLIRRFKKDHKSFQALPDRVAVQLNDTHPTIGVAELMRI
                     LVDEEGIPWDEAWAISTKVFAYTNHTVLPEALETWPVGLLGRVLPRHLEIIFEINHRF
                     LDWVRKERPGDEGLLSRVSIIEEGPVKKVRMANLAIVGSHSVNGVAALHSEILKKSLF
                     RDFHELYPGKFKNITNGITQRRWLLQANPLLSDLITKTIGPDWIVDLYKLEKLLPHAE
                     DKAFRTEWMKVKRANKERFSTYVYEQTGVRPDPDAMFDMQTKRIHEYKRQVLKVLHVI
                     TLYNRIREGKTIAPRAVFIGGKSAPAYYMAKLTIKLVNSVANVINHDPAMHGKLQLVF
                     LPNYCVSQAEKVMPAADLSEQISTAGMEASGTGNMKFSLNGALTIGTLDGANIEIMEE
                     VGPENFFVFGLKSEEVDHMRATGYRSRDYYERDPELKAALDMIGSGAFSPEQRDLFLP
                     IVQGLLDRGDRFFVLADYRSYITAQESVGMLYRDLDAWGRKSVLNVAKMGKFSSDRSV
                     REYAEKIWNIPTTR"
     gene            complement(6141..6719)
                     /locus_tag="PLG78_00530"
     CDS             complement(6141..6719)
                     /locus_tag="PLG78_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE21618.1"
                     /translation="MKEKQGPRERIVSAAGSLFYRRGFGTGINEILGESGAHKLSLYQ
                     YFPSKDDLGREYVERQKEQILGFLAKLIQERPDPRDFIKSWVQVIKRQARKKEFFGCP
                     FANFAAQTLEKKPEFRDQLGGVLLEWQQMFSDYFAKWQASQSRSGRVSPAQLARRTLA
                     IYEGNVQLYLISGDAGYLDAIEADFVEMIRST"
     gene            6838..7722
                     /locus_tag="PLG78_00535"
     CDS             6838..7722
                     /locus_tag="PLG78_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNE21619.1"
                     /translation="MRKISILLFLAATSCAHQGFVFRSVHGFSPATNAALEKFLEENK
                     SHKGRKVAVFDGDGTVLGQVPHYLADECLYRYAKAHPDRRPEIIARMKHKSNVSIPYV
                     QDRVFYLAGMTLDEVRQMGTQCYESDYRGKIYTPMKDLIELLKDNDFEIWIITASPEG
                     LYQEFLSHELQISPTHIVGVKSVIRGGIITDRIVEPIPQDAGKKEAVETFVQTTPLLA
                     AGNSRGDREMIESSSGIRMIINPDEHLESGEKISIAQYAKDHNWLVEKINDVEESGFP
                     AVSSKSYGIRKNRAHPVP"
     gene            complement(7726..8616)
                     /locus_tag="PLG78_00540"
     CDS             complement(7726..8616)
                     /locus_tag="PLG78_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE21620.1"
                     /translation="MFSSRALKWIVGLPAAIATLLVAVAWYFSSLVLYPPVHCNPDHH
                     VYCGNPSEIGLAFEDVQIPTSDGLKMQSWYIPAERSTKAIVMVHGHGGLKNEGLRFSR
                     ALHDAGYNLLLLDLRRNSGGSATMGYLESRDVIRALDFLSTEKKNEKLGIFGFSMGAA
                     TSILSMAADQRVRAGLFSSGYASAVDVLSEAAARDYHLPYYPLFPVMLFVGDHRAHIE
                     LEAVRPVDVIGGISPRPISIFHCDKDDYVESSHAQRLFAAAREPKEIWTPPCNRHERI
                     WNVHREEAERRAVAFFKKNL"
     gene            complement(9028..11211)
                     /locus_tag="PLG78_00545"
     CDS             complement(9028..11211)
                     /locus_tag="PLG78_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE21621.1"
                     /translation="MRAYVKSGDVWGGLAAMLVALPQSIAFGITAFSALDPGLLAQGA
                     MAGIIGAIAVGLVAPIFGGTPRLISSPCAPAAAFLAGLASEGWMKSAFAGGPGPAIAL
                     VILVTFSAGLLQLLFAFLGGGKVIKYIPYPVVAGYMSSVGIIIVIGQIPRILGSTPLA
                     EFEHGVSPVHLAAAIIATATIAAMFIVPRITSVMPAPIFGLGAGLVAYGLFALAFPEY
                     RSLEGNVLVVGPLHGEFSFDVFFSRFRSYATLQGDYLREAVLAGGTLAVILSIDTLKS
                     CVVVDTVTRSRSDSNRELLGQGLGNITSAFCGGLAGAGTLGATLVNVSSGGVTRISGV
                     LAGLFALIALVFLSPVFAWVPIPALSGILIVVGLRMIDWKLAALLRRKATALDFFVIL
                     TVIGVALKFSLMAAAGAGVALSALFFMREQMRGSVVHKKTYGSEISSKRSRLPAEQKV
                     IRASGQRAVLYELQGSLFFGNTDRLFTLIHADLEESQFVIISMRRVQSIDSTAIHMLL
                     QINTLLEDRGGRLFITDLPESLEEGQAPEEFLHDAGLLGPASGIVLFDEFYYALEWVE
                     DYILKENDLYHSHLERPLELGEIEVFENVDAEDLPLIQQCLESRSFSAGNTIFSSGDT
                     SDNLFFIRRGVVRIMLPINSHRQHHLASFGRGDFFGDMAFVDHEVRSAHAIAETDCDL
                     YSLSRNVFDSLLAKDPQIGRQFYENLVRVLAHRFRAVHLELRSLE"
     gene            11308..11664
                     /locus_tag="PLG78_00550"
     CDS             11308..11664
                     /locus_tag="PLG78_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21622.1"
                     /translation="MKRVLIVDDSMIMRFVLTTHLKGLPIEIVAEATNGREALELFSK
                     HKPDIVTMDITMPEMDGLTSMKKMIQILPETKVIVVTAVSDPAVALDAMEKGAYAVIH
                     KPVTAAQMQEIMSTLV"
     gene            11669..14113
                     /locus_tag="PLG78_00555"
     CDS             11669..14113
                     /locus_tag="PLG78_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21623.1"
                     /translation="MMESLSFAGPENRVFWAVVLIALIAVAILVGVLSRKRFGMKLGL
                     AFTLLSVTVTSVSVFFYYNAMYDSLYASTTARIKDIGRTGLFLFDDEIKETILRIHRE
                     SEQKSKTRDAAFLSKITRGNSDNSLSDEDIAAILQDKGFIRLVQVLRKIKNGTREHVV
                     LTAPIPQEPLDPNDLPVIRYANVFVPIPESPSVEHVKWIADTDCERLDANKNGKIDDD
                     EQASSPGMIYNYGDASAEIKGALAGEVTATKGYIFDTYGVWVSAFFPIYHEGKVIAVH
                     SMDLDARREVNLVRKLWFQSLGIIALSFVLSIVFSILMSKYLARPIMQMSQAARRIRA
                     HDYSTRITMRRKDEFANLKDAFNEMAAEVGAYAQTLEQRVAERTSELEQARKETGRIL
                     ATVSDALFLLTPEGTLRGNYSRAMEDIFPGQRIAGANLMDLLRPVIAPQTLLSVQTYL
                     KLMFDSSRPDVTLKGLNPLARIQFSDGSGIWVSSNFQRIHSDGKVTELLGIFSDITPQ
                     IRLQEELGQEKRRSEIQTKLVLDLLRVAPKLLRMFLSELEADLPEVKQSISDQNRLTV
                     MRRLHTLKGNAASLGLDMFAEALHVAEEAAEKSSSDPTPYSAAIATVEQIHKESSALS
                     EKIRDAATIQVDENFIVQAIRGIFERRGHKIDLVTTNFDAKLIASKHERMIRDALIQF
                     ARNTIAHGWESPQERESNGKQSEMIITLSPRELNGHIEVTYSDDGKGLDVEKIRKRGI
                     SLGLLSETDPVDGARAVELIFHPGFSTRTSSEISIDAGRGVGLDLVRAAVEEAGGHIR
                     TASNNQRTEFTIELPV"
     gene            14126..15253
                     /locus_tag="PLG78_00560"
     CDS             14126..15253
                     /locus_tag="PLG78_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21624.1"
                     /translation="MKLAFLLLSAAWLALVVAAAAAFYRFRSSLGKLRKSAEAIGRGD
                     FSISVLTDERGHLGDFARVFDGMRVDLAQAALAIREVAGDRDATQERYKIIYERTGDP
                     IFTILPNLQITAANPAAARLIGTQKGALKGKNFFDLVSTENQGSLAPVILMKEFAEFI
                     ENRGSMARRMVFRRLDGKETPEYDVRLEFLNVEGVDEIIARAVPVRTDALLHGLVKES
                     LSFEMKSDLLFVDTLAQRLSRNVPRYCAEEESFNVTLGVREMLINAIEHGNLGITHSE
                     KSAAMSQDTYLDLLQERARNPAYGSKVVWVEYELDAQKVRYRITDQGRGFDHKSFTKQ
                     QMPDVRRPHGRGILITSECFDSVTYNETGNSVELIRFFPTA"
     gene            complement(15306..15704)
                     /locus_tag="PLG78_00565"
     CDS             complement(15306..15704)
                     /locus_tag="PLG78_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21625.1"
                     /translation="MKKNLLVSLALVSLTLSLPLFAQTPPPERKPSPECKAMHEKMME
                     GRKARDAKLEKMLATLKTATGQKKVDVMSDILTELLTERKEMQAHMQQMMEMRKNGNG
                     CEMMGPGHPMMDHRSGPGQDRPAPDHSEHH"
     gene            complement(15829..16947)
                     /locus_tag="PLG78_00570"
     CDS             complement(15829..16947)
                     /locus_tag="PLG78_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21626.1"
                     /translation="MAESENDILTRRESIVVTVDDDPAILDLIRHYLGHAGIQPVTFN
                     NAEEALQHLPDLHPDCILLDFSLPGMSGIQTCMKIREHASMRETPIIFLSADEDKKRV
                     VQALTAGANDYITKPVHEQELIVRIETHIELYLQKQRMLEQRRLLERRNEENIILNTR
                     SLMLQEVIRQFTPRSTWEKADLAAGEGMIGIADEEIDMTYFFLDIRSFTRFSDTHPAQ
                     EVIHSLNEILAPVTDIIYEEGGDVDKFIGDSIFGTFSDPLSAARAAIRSRDAIAKINA
                     NRRASGKAELGARIGMNSGRAIRGNVGSTIRKENTLIGDAVNVASRLEHACEPGRVMI
                     SAVLYERVRGKVTVSRPYLLKAKGKSEAIRVYYLEAIQ"
     gene            complement(17013..>17447)
                     /locus_tag="PLG78_00575"
     CDS             complement(17013..>17447)
                     /locus_tag="PLG78_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21627.1"
                     /translation="RLSGLVLLVVVLFGLEACHRARESAPVDSPVVQANPDTLPGEYS
                     GDREWSLARDRWFKETYGPCMKSAGLRMSCGGCASIYIKFVFTTNEDGRIISMEKYKE
                     NVCGAPPTAELERCMTAPYQGRVFSKLRSRKFRATLGTGLSC"
BASE COUNT         4497 a         4511 c         4253 g         4186 t
ORIGIN      
        1 acagcaaagc ggatggactc attccgtgga aacattcaga ggaattgcat gctctgtaca
       61 atggaccaaa aaaactctgg atcacggaaa cgggagagca tgcagctgta tggaacactc
      121 acaaagaaga gtacgaacga gcattgactg atttcttgaa tcagccaccc atcattgctc
      181 cacgcagatg agtggcttga gcgtatcgca cgtgttggtg gtaatggaaa tcgcattgga
      241 acccgtcgag gatccatctc cgacgcttcc actcactgcg attccattgt ccgtccaact
      301 cgtacagtgc gatccagtgg tccagtctcc attgaggcct gtccagtaag tgcttcctgc
      361 tccaaaattg tttgtaagac taccaaaaac aaaaatggaa ttcgcatctg atgtgaacaa
      421 tgtcgcccca cctggatatc gaatgtagtc gtaaagagga gaaagcaccc aggatatttg
      481 ggagtctcct agatttgcag tcagagacgc acgcctgtga cttgcgtcta ctgcgagagc
      541 tctgaatgtt ccactcgctg gtttgttcgc gtcagtctga catgcattgt ccatttcctg
      601 tcttggattg ccgtctccgt tcactgccga attcgccccg ccggcaagag atgcattgaa
      661 atcaaatgca ccatcatacg tactggcggt gatgaagatg tacttctcat tgtccacatt
      721 tgtaactgca acatctgctg gattgatcaa tgagtacaca ggatccgctg tggactgagc
      781 tgtgatgata gagtaaccga tgtttccgtc cttcgcggta tcgtttacgc cggtcacagt
      841 cacagtctga ttcgtgcacc aatcgcctcc gcccatgcac gctcccacgg ttgcatcgaa
      901 tgtgacgctt gctggagaca ctgtgccttc ggcgacattc gaactggaaa ggttgactgt
      961 aacattgttt gtaggtttag aattcaaacg tatcgtgaaa gtagccgtgc cacccgcttc
     1021 tgttgtgttt ccgctgatcg cagatactgt gtagcccgca gtgtcattgt cattgttcgt
     1081 aacattcgga tccgccagat caattccatt gaaagcagcg tccccactcg tggctgcacc
     1141 aatcgtcaaa gttcgcgtct ggttgccatc atccacagca tcgtctacgc ctgtgagagt
     1201 gacctgtctg caagtgttcc agtttgcagg cgtgaaagta agtgtagctg gagaaactgt
     1261 gaattctgtt gtatctgtgc ttgagaaagg aatatccaca ttcgccgaag gcgcctgcgt
     1321 gagagtgtag aacatcagcg ccgttccgcc cgcttccgtt gttgtgaggg agttggaatc
     1381 acatcgcacg atttttgtag caccgtttat gtccgcattg cgaatggtga cctgtcccgc
     1441 agccccgccg ttataatccg taggcgtaat tccattgtag ttcaaatcag tgctcgttgt
     1501 ggttcccaga tccacatggt agatgatggt gccatccacc actgcatccg ggaggccggt
     1561 cactgttaca tcctgagcaa cattccagtt ggcgctggtg aaagttaagg tacctggaga
     1621 aacagaagct tccgttccat catccactgt gatcgggcca acgtccacat cctggagtgg
     1681 ctgagcggat agaactactt tcaagattac ctgaccgccg ccctctgtcg tatacagatt
     1741 gtttgtactg gaagaaatat tcaccaggtt ttgatcgttg tcattgtttg tgacgctcac
     1801 atccggaggg ttgagtccac tgtaatttgg atctgcactg actgcggggg ccgtcacaat
     1861 cgtataagca atgtttccat cgaggatcgc gtcgttgacg ccgaatactg tgacagtctg
     1921 cggagttgcg aaattcgcgt tggtgaaagt caaagtcgcg ggagcaactg ttccttctgt
     1981 tgtgtctgaa ctggaaatcc ctactacaac gttagacgat ggctgctgag tgagaacgac
     2041 cgtgaaggta gcgttgactc caccttctcc agtgaccaga ccgctggtcg gactgacaac
     2101 aatgtccgga atccctgcgt ccgcattggc tacactcacc ggagacacga tcatgccgct
     2161 gtaattcgca tcggcactca ccactggtcc cagagttaca ttgtaagtga tggttccatc
     2221 cagaatcgca tctgcctgcg gcgtgactgt aaaggactgc ggcacgttcc aatttccggc
     2281 agtgaatgtc cgactcggag gtgcaacggt tccttctgta gtatccgcac tggcagcaga
     2341 aatcgtaaca ctggaagttg gctgcgaagt gagcacgaca gaaaaagttt ttgcgacacc
     2401 gctttcgacg gtagacaaac ttgttggagt cacagtcacc cctgcagtgt catcatccgt
     2461 agtggtaacg gccacagtgt ccgtggtccc ggtagccacc caggccgcgt tagacgctgc
     2521 attgatgcca agagcaacgt tatacgctat tgatccatct tgtaagaaat cgttcacacc
     2581 tgtgacagtc accgtttgcg cggtggacca gtttgcggga gtgaatgtaa gactcgcggg
     2641 actgactgtt ccttctgtag tatcaagcga agtaacgtcc acaatcacgt tagaggcagg
     2701 agcggaactg agtagaacgg aaaaagtggc agtggttccg ctttcagcta cagtcaatcc
     2761 agtcaaaggc catgcggaca ctcttctttg ctcgtcgtcg agattgacgc agctcacaga
     2821 accgacagac aaattgttat aggaaggatc ataactcgat gcattgccca gcgcaattgt
     2881 gtagctctgg tttccatcta ccgatccatc atccacacca gaaacggtaa tcgtttgcgt
     2941 tccgccggca acagatagac tcacgctgct cggcgaaacc acaccttccg tggtcagaag
     3001 agaacttaca ggaacggtga ccagggcacc gggagttgta tttgtctcca cggtgaaagt
     3061 gtgattcaat cctccttcgg atgtgataca aggcgtggat tggcgaatcg taaaccctgg
     3121 agttccgccg ctgcgaaaga aagcagggtt caggaaagta acagcaaagt agccggtcac
     3181 ggcatcatac tggccgcaag aagaaaagag acataagcaa accagaccgc agctctttgc
     3241 aaactttccc atggtgctaa ttgaaagatc ggcatggaac ctggggcttg acaagccatt
     3301 ttctacatgc agtcgagaca gataatcatt cagcttctgc aaagcttcct ttaccgcaga
     3361 agcatcgctt gcatgtgctt tctcttcaat caatttgcca atggtagaaa tctcatccag
     3421 accatatccg ccaccggaac ccttcagatt gtgaccgatt cgtccagccg ccgccaaatc
     3481 ccccgccgag attagctctt tgagctggtc agcatatttg ctgagattct tgagataccc
     3541 gggtatcagc tcagaaagat ctgaaggcac ttgaactacg atcttatcca tggcttccct
     3601 ctctcatttc aacgagtggt cggaatattc cagatttttt ccgcatactc tctcacggat
     3661 cggtcactgg agaattttcc catttttgcg acgttgagaa ccgacttgcg accccaggca
     3721 tccagatcgc gatacagcat ccccacactc tcctgagctg tgatgtaaga acgatagtct
     3781 gccagcacga agaaacgatc cccgcgatcc aacaggccct gaacgatagg caagaataga
     3841 tctctttgct caggcgaaaa tgcgcctgaa ccaatcatat ccagtgccgc tttgagttca
     3901 ggatctcttt cgtagtagtc acgtgatcga tagccagtgg cgcgcatgtg atccacttcc
     3961 tctgatttga gtccaaacac aaaaaagttt tccggtccca cttcttccat gatttcaata
     4021 ttcgcgccgt ccagtgttcc aatggtcaga gcaccgttca gcgaaaattt catgttccct
     4081 gttccggatg cttccatccc ggctgtagaa atctgttccg ataggtccgc cgccggcatt
     4141 actttctccg cctgcgaaac acaatagttc ggaagaaaca cgagctgaag tttaccgtgc
     4201 atcgcgggat catgattgat gacgtttgcg actgaattca cgagcttgat cgtcagcttg
     4261 gccatgtaat aggcaggtgc agattttcca ccaatgaaca cagcgcgcgg tgcgattgtt
     4321 tttccttcgc gaatcctatt gtaaagagtg atgacgtgca gcactttcag aacctgccgt
     4381 ttgtattcgt ggattctttt tgtctgcata tcgaacatgg catccggatc tggacgcact
     4441 cctgtctgct cgtacacata ggtgctaaat ctttccttgt ttgctctttt gaccttcatc
     4501 cactcagtgc gaaacgcttt gtcttccgcg tgggggagaa gtttttccag tttgtacagg
     4561 tccacgatcc aatccggccc aatggttttt gtgatcaggt ctgacagaag tggatttgct
     4621 tgaagcagcc agcggcgctg cgtaattcca tttgtaatat tcttaaactt tcccggatac
     4681 agctcatgaa agtctcgaaa cagactcttt ttcaagattt cagaatgaag tgccgcgact
     4741 ccatttactg agtgacttcc aacaattgca agattggcca tccgcacttt ctttacagga
     4801 ccttcttcaa tgatagacac gcgcgaaagc agcccttcat cccccggtct ttctttgcga
     4861 acccagtcga gaaatctgtg attgatctcg aaaatgattt caagatgcct tgggagcact
     4921 cgcccaagca agccaaccgg ccaggtctca agtgcttccg ggagaacagt gtgattggtg
     4981 tatgcgaaaa ctttcgtact gatggcccag gcttcatccc agggaattcc ctcttcatcc
     5041 accagtatgc gcatcaactc tgcaacgcca atggtgggat gagtgtcatt caattgaacg
     5101 gcaactcgat ccgggagagc ctggaaagat ttgtggtcct tcttgaatct tctaattaaa
     5161 tcttcaaaag tagccgccac aaagaagtac tgctgcttga ggcgaagctc tcggccctgt
     5221 tccttttcat cgcttggata cagcaccttc gagatgttct ccgaaagaac tttctcttcc
     5281 atcgcgcgaa cgtagtcccc gtgattaaag aaatccagat tgaaatcacg actggatacg
     5341 gccgcccaca agcgcatgtt gtttacagtt tcgttgttgt atcctggaat gggaatgtcg
     5401 caggccatcg catccacggt atcggtgtcc acccagcgat agcgaagatt tcctctttca
     5461 tcatgatatg gttctgacct gccaaatagt ttcacagtat acagaaagtg tctgcgcgtg
     5521 atttcccacg gatttccaaa ctgaagccag ttgtcgcatt gctccacctg ccagccgttc
     5581 acaatcttct gatgaaaaat cccgtagtcg tatcgaattc catatccatg cgctgcaatg
     5641 ttcaagcagg ccatggaatc cagatagcag gatgcgagac gacccagacc accgttgccc
     5701 agaccaggat ccggctcctg ctcttccagt tcttcaagtg cgaaacctga ttccttcagc
     5761 gcttcgcgcg attcagcctc catgccaagg ttgataataa agtttttcaa gaatctgcct
     5821 ggaagaaact ccatggacat atagtaaacg cgcttgggat gtgtttcgta agcgagtctt
     5881 tgtccagaaa gccagcgctc aatgagtcta tcacgcacgc agtaggcaag accactgtag
     5941 taggtgtctt tgcgaggagg ctgaaagtca tttccaagta caaaaacaat atgacgtttg
     6001 atgctattgc ctagatcttc ccagtgcgat tgcgatacag gttccataac tcatgatgcg
     6061 ccaggtttgc aaaaggaagc aaccgtaaaa ccaggaggcg tcagacgaaa aatgtcatgg
     6121 ttcttctgtc gaaagttact tcaggtgctc cttatcattt caacgaaatc agcttcgatt
     6181 gcatccagat aaccagcgtc tcctgaaatc aagtagagct gcacattgcc ttcataaata
     6241 gcaagagtcc gcctggcaag ttgagcgggc gaaactcgac cagagcgaga ttgagaggcc
     6301 tgccacttcg cgaaataatc agaaaacatt tgctgccatt ccaacagtac cccgccgagt
     6361 tgatctcgaa attcaggttt cttttccaga gtctgagccg caaaattggc gaatggacaa
     6421 ccaaagaact cctttttcct ggcctgcctt ttgatcacct gcacccagct cttgatgaaa
     6481 tcacgtgggt ccggacgttc ctggatgagc ttcgcaagaa aaccgagtat ctgctctttc
     6541 tgcctttcta catactcacg acccaggtca tccttggacg gaaagtactg atacaggctg
     6601 agcttgtgtg cgccagattc tcccagaatc tcgttgatac ccgttccaaa cccgcgcctg
     6661 taaaaaaggg aaccggccgc gctgacgatg cgttcccggg ggccttgctt ctccttcata
     6721 gcctccgaca aaaagaaggc tggaccgatc tgtctagtcc aattcggctc gaatcgccaa
     6781 aaaccgcgat gcatttttgc tcgacgcgct cgaatgcaca ctcccaccat caactcaatg
     6841 cgcaagattt ctattcttct gttcctggcc gcaacttcct gtgcacacca gggttttgtg
     6901 tttcgcagcg tccacgggtt cagtcccgca acaaatgcag cgcttgaaaa atttctggaa
     6961 gagaacaaat cgcataaggg tcgtaaggtg gccgtatttg acggagatgg aacggtgctc
     7021 ggacaggtcc ctcattacct ggcggacgaa tgtctgtatc gctatgcgaa agcgcatcca
     7081 gatagaagac ctgaaatcat cgcacgcatg aaacacaaat cgaacgtttc tattccgtat
     7141 gtgcaggacc gcgtttttta tctggcaggg atgactctgg acgaagtaag acaaatgggc
     7201 acgcaatgct acgagagtga ttatcgcggc aaaatatata ctcccatgaa agatctgatt
     7261 gaactattaa aggataacga ctttgaaatc tggattatca cagcaagtcc agaaggattg
     7321 tatcaggaat tcttaagtca cgaactgcag atttcgccaa ctcacatcgt tggtgtgaag
     7381 tctgtcattc gaggcggaat cattacagat cgaatcgtgg aacccattcc gcaagatgcg
     7441 gggaaaaaag aagctgtaga aacttttgtc cagaccaccc ctctcctggc cgccggtaac
     7501 agtcgagggg atcgcgaaat gatcgagtcg agcagcggaa tccgtatgat catcaatcca
     7561 gacgagcatc ttgagtccgg cgaaaaaatc agcatcgcac agtacgcgaa ggatcacaac
     7621 tggctagttg aaaagatcaa tgatgtagaa gaaagcgggt ttcctgccgt atcttcgaag
     7681 agttacggca tcagaaaaaa cagagcccac cctgttccat agcatctaaa gattcttctt
     7741 gaagaaagca accgcgcggc gttccgcctc ttcgcggtga acgttccaga tgcgttcatg
     7801 tcgattgcat ggtggagtcc agatctcttt gggttccctg gcagcggcaa aaagcctctg
     7861 cgcatgagac gattcgacat agtcgtcttt gtcgcaatga aagatggaaa ttggacgcgg
     7921 actgatgcct ccgatcacat ccacgggacg caccgcttcg agttcaatgt gagcgcgatg
     7981 atctcctaca aacaacatca caggaaacag aggatagtac ggaaggtgat agtcacgggc
     8041 tgccgcctct gacagaacat ctactgcgct cgcataaccg ctgctaaaga gcccggcgcg
     8101 aactctctga tctgctgcca tggataggat gctcgtggca gcacccatgg aaaaaccaaa
     8161 gatacccagc ttttcgttct tcttctctgt cgaaagaaaa tcgagggctc gaatcacgtc
     8221 tctggattca agatatccca tggtggccga tccaccgcta ttccttcgca gatcaagcaa
     8281 gagtaaattg tagcccgcat catggagcgc gcgcgaaaag cgaagacctt cgttcttcag
     8341 tccaccgtgg ccatgcacca tcacgatggc ttttgtagaa cgctctgccg ggatatacca
     8401 gctctgcatc ttcaacccgt ctgaggtagg aatctgcacg tcctcaaagg cgaggccaat
     8461 ttcagaagga ttgccacaat aaacgtgatg gtccggattg caatgcaccg gagggtacaa
     8521 caccagagac gaaaaatacc aggcaacggc gacgagaagt gtggcgatgg ccgccggcag
     8581 acccacgatc catttcaaag cgcgtgatga aaacatcgcc gaaagtcatt gcgattgaaa
     8641 tatttgcaaa ccgattttat tcctcgggag gatctaccag ttcgcaattc ctgatcacgt
     8701 aaaccccgcc gtctgtaccc tgactgattt caccattttt ttttgtcgcg gcgaacgcat
     8761 ccgtgtactc gagggttcct cggaccgtta tgcgctttcc atccaacgtc tttttccacg
     8821 agggacgatt gagacagtag acactcaatt ccggcgactc cacatgggcg gaaattttgg
     8881 cgttcccggc aatgccagtc aagacgatgg gcttcccgag ggacctgtgc cttccccctg
     8941 cctgagacga actgcccgca caggcggcga tcaggcccag cgccagggtt ggcgcaaaga
     9001 gacttcggat gcaaagtcga ctcatattca ctccagcgat cgaagctcca gatgcaccgc
     9061 tctgaaccga tgagccagaa cgcgcacaag gttttcgtag aactgtcttc caatctgtgg
     9121 atcttttgca agcaaagaat caaatacgtt acgggaaaga ctgtacaaat cgcaatccgt
     9181 ttcagcgatt gcatgcgctg aacggacttc atgatccaca aaagccatat ctccgaaaaa
     9241 gtctccgcga ccaaaagagg ccaggtgatg ttgcctgtga ctgttgatgg gcagcatgat
     9301 tcgcactaca ccgcgtcgaa tgaaaaacaa attgtcgctt gtgtctccgg atgagaagat
     9361 cgtattgccc gcgctaaatg atctagattc cagacattgt tgaatcaagg gcaaatcttc
     9421 cgcatccaca ttctcaaaga cttcgatttc gcctaattca aggggccgct caagatgaga
     9481 atgatacaaa tcgttctctt tcaaaatgta gtcttccacc cattccagtg cgtagtaaaa
     9541 ttcatcaaac aatacgatgc ctgaagcagg cccgagaagc ccggcatcgt gcaaaaattc
     9601 ttccggcgcc tgtccctctt ctagagattc cggcagatcc gtgatgaaaa gtcgtccacc
     9661 gcgatcttcc agtagagtgt tgatttgcaa aagcatgtga atggcggtag aatcgatgga
     9721 ctgaactcga cgcatactga tgattacgaa ttgcgattct tcaagatctg catgaatcag
     9781 agtgaacaat cgatctgtat ttccaaaaaa cagactgccc tgcaattcat acagcaccgc
     9841 acgctgcccg ctggcccgaa tcactttctg ttctgcggga agtctgcttc tcttcgaaga
     9901 tatttcgctt ccgtatgttt tcttatgcac gaccgatccg cgcatttgtt ctctcatgaa
     9961 aaagagcgcg gataacgcga cgcccgcacc tgccgccgcc atcaagctga acttcagtgc
    10021 cacgccgatg acggtcagga tcacgaagaa atccagggca gttgcttttc tgcgaagaag
    10081 cgccgccagc ttccagtcaa tcattcgcaa accaactacg atcagaattc cagacagagc
    10141 gggaatcgga acccatgcaa aaactggaga aagaaataca agagcgatga gcgcaaagag
    10201 tccggcaaga acgccagaga ttcgtgtgac accgccgctg gaaacattga ccagtgtagc
    10261 tcccagagtt cccgcaccgg ccaggccacc gcagaacgca gaagtgatat ttcccagacc
    10321 ttgcccgagc agttctctat tagaatcgct gcgagatctg gtcaccgtat ccaccacaac
    10381 gcaggacttg agagtatcga tggacagaat gactgccaga gttccaccgg caagaaccgc
    10441 ctcccgcaaa taatctccct gcaatgtcgc gtaacttctg aacctggaaa agaagacatc
    10501 gaaagagaac tctccgtgca gtggtccaac caccagcacg tttccctcca atgatcgata
    10561 ctccggaaaa gccagagcga acagaccgta ggccaccaga ccggcgccaa gaccgaagat
    10621 cggcgccggc ataacagatg ttatgcgagg aacaatgaac atagccgcaa tggtggctgt
    10681 agcaatgatt gctgcggcca gatgaaccgg cgaaacaccg tgctcgaact cagcaagtgg
    10741 agtggacccg agaattcgcg gtatctggcc aattacaatg atgatcccca cactgctcat
    10801 gtaacccgca acgactggat atggtatgta ttttataact ttccctccgc caagaaaagc
    10861 aaagaggagc tgcaacaacc cggctgaaaa agtgaccagg atcacaagag caatggcagg
    10921 ccccggtcca ccggcgaaag cggatttcat ccacccctcg gacgcgagtc ctgctagaaa
    10981 tgcagccgca ggagcacagg gagaagaaat caagcgggga gttccgccaa agatcggagc
    11041 aaccaggcca actgcgatcg ccccaatgat tccagccatc gcgccctgtg ccaggagacc
    11101 aggatcaagc gcggagaatg cagtaatacc gaatgcaatg gattgcggaa gcgcaaccag
    11161 catcgcagcc agaccgcccc acacatcccc tgattttaca taagctcgca tgtccgtgcg
    11221 ctctatgacg aatgtttccc tcgaaggacg ggctgcgagg acaaaattct gtttactgtt
    11281 ctctgcgaaa cgaaaggctg agcagagatg aaacgggtct tgatcgtaga cgattcgatg
    11341 atcatgcgat tcgtcctgac cacacatttg aaaggcctcc ccattgaaat tgtcgcggaa
    11401 gctacgaatg gccgcgaagc cctggaatta ttctcaaagc acaaaccaga tattgtcacg
    11461 atggacatca ccatgccgga aatggacggg ttgacttcca tgaagaaaat gattcagatc
    11521 ttgccggaaa caaaagtcat cgtggtgact gcggtgtcag acccggccgt ggccctggac
    11581 gcgatggaaa aaggcgctta cgctgtgatt cacaaaccag tcaccgcagc acagatgcaa
    11641 gaaatcatgt ccactctggt ataaggaaat gatggagtct ctttctttcg ccggcccgga
    11701 gaatcgcgtt ttctgggctg tcgtgttgat tgcactcatt gcggtcgcca tccttgtggg
    11761 cgtcctttcg cgaaaacgat tcgggatgaa actcggtctt gccttcaccc ttctatctgt
    11821 gaccgtcaca tctgtcagcg ttttcttcta ttacaacgcg atgtacgatt ctctctacgc
    11881 ttccacgacg gcgcgaatca aagacatagg tcgaaccggg ctatttttgt tcgacgatga
    11941 aataaaagaa accatccttc gcatccaccg cgagagcgag caaaaatcaa aaaccaggga
    12001 cgctgctttc ctgtccaaaa taactcgagg caattcggac aattctctgt ctgatgaaga
    12061 tattgctgcc attttacagg acaaaggatt cattcgcctg gttcaggtgc ttcgaaaaat
    12121 caagaacgga accagagagc acgtagtctt aacagcaccc attccccagg agccactcga
    12181 tccaaatgat cttccggtga ttcgctacgc caacgtattt gttcccatcc ctgaatcgcc
    12241 gagcgttgag catgtgaaat ggattgctga tactgattgt gaacgattag acgcgaacaa
    12301 gaacgggaag atagacgatg atgaacaggc atcttctcca ggaatgatct acaattatgg
    12361 agacgcttcc gcggaaatca aaggagctct cgcgggagaa gtcactgcca caaaaggata
    12421 tatattcgat acgtatggcg tctgggtcag cgcgtttttc ccgatctatc atgaggggaa
    12481 agtcattgcc gttcattcca tggacctgga cgcgcggcgc gaagtcaatc tggtgcgtaa
    12541 gctctggttt caatccctcg gaatcattgc gctttccttt gttctttcga ttgttttttc
    12601 cattctcatg agcaagtatc tggcaaggcc aatcatgcag atgagtcagg ctgccaggag
    12661 aattcgggca catgattatt ctacccgaat cactatgcga agaaaagacg aattcgccaa
    12721 tctcaaagat gcattcaacg agatggcggc agaagttggc gcatacgccc agacgctcga
    12781 acagagagtg gcagaaagaa catcagaact tgagcaagcg cgaaaggaaa ccgggagaat
    12841 tctcgcgact gtatcagacg ctctcttcct tcttacgccg gagggaaccc ttcgcggaaa
    12901 ctactctcgc gccatggaag atattttccc gggccaaagg atcgcaggtg cgaatctgat
    12961 ggaccttctt cgtcctgtca ttgcgccgca aactcttctc agcgtgcaga cgtacttgaa
    13021 actgatgttt gacagctcac gtccggatgt aacactcaaa ggactcaacc cactcgcgag
    13081 aatacagttt tctgatggat ctggaatatg ggtgtcctcg aacttccagc gcattcacag
    13141 tgatgggaaa gtcactgaac tgctcgggat cttctcggac atcaccccac agattcgcct
    13201 ccaggaagaa ctgggacagg agaaaagacg cagcgaaatc cagaccaaac tggttctcga
    13261 tttgttgcga gtcgctccca aattgctgag aatgtttttg tcagaactgg aagcggatct
    13321 tcctgaagta aagcaatcga tctcagatca gaatcgccta acggttatga gaaggttgca
    13381 cacattgaaa gggaacgcag cgtcccttgg tcttgacatg tttgcggaag cactgcacgt
    13441 cgcggaagaa gccgcagaaa aatcatccag tgatccaact ccgtactccg cggccatcgc
    13501 gaccgtggaa caaatccaca aagaatcgag cgctctgtcg gagaagatta gagacgcagc
    13561 cacgattcaa gtggatgaaa acttcattgt ccaggcaatt cgcggaatct tcgaacgcag
    13621 agggcataaa attgatctgg tcactacgaa ttttgacgcg aagctcattg cttcaaagca
    13681 tgaaagaatg attcgagatg cgctcatcca gttcgcccgc aatacaatcg cgcatggctg
    13741 ggaaagcccg caggaacgcg aaagcaatgg caagcagagt gaaatgatca ttactctatc
    13801 ccccagagaa ttaaacggtc atattgaagt tacatatagt gatgacggaa aagggctaga
    13861 tgttgaaaaa atcagaaaac gcggaatctc tctagggctc ctgtcagaga ccgatcccgt
    13921 ggatggagct cgcgctgttg agctgatttt tcaccccggg ttttccacgc ggacaagctc
    13981 cgaaatttca atagatgcag ggcgaggagt cggcctggac ctggttcgag cagcagtcga
    14041 ggaggcggga gggcacatcc gaactgcatc aaacaatcag cgtacggagt ttacaatcga
    14101 gcttccagta taaaactgcg cacagatgaa gttggccttc ctgctgctct ccgctgcatg
    14161 gcttgcactt gttgtggccg ccgctgctgc gttctatcgt tttcgttcca gcctgggcaa
    14221 gcttcgcaaa tctgcggaag cgattggccg gggtgatttc agcatttcgg tactcacgga
    14281 cgaaagaggt cacctcggag atttcgcccg tgtgtttgac ggaatgcgcg tggatcttgc
    14341 gcaagcggct cttgccattc gagaagtggc gggcgatcgc gatgcaacac aggaacgcta
    14401 caagatcatt tacgaaagaa cgggggatcc tattttcacg attctcccga atcttcaaat
    14461 tacagcggcg aacccggcag cagctcggct catcggaacg cagaaaggcg cactgaaagg
    14521 aaaaaatttt tttgatctgg tgagcacaga gaaccaggga tcgctggcac cggtcatact
    14581 catgaaagaa ttcgcggagt tcatagaaaa tcgcggttcg atggctcgtc gtatggtctt
    14641 tcgtcgttta gacggaaaag aaactccgga gtacgacgtg cgtctggaat tcttgaatgt
    14701 agaaggagta gatgaaatca tcgcgcgcgc ggtccccgta agaacagacg cgcttctgca
    14761 cggcctcgtg aaagaaagtc tctcctttga gatgaagagc gatcttctat tcgtggacac
    14821 tcttgcgcag aggctctcgc gtaacgttcc ccgctattgc gcggaagaag aaagcttcaa
    14881 tgttacactg ggtgtgcgcg aaatgctcat caatgcaatt gaacatggaa atcttggaat
    14941 cacgcactca gaaaagagtg cggccatgtc tcaggacacg tacctcgatc tacttcaaga
    15001 acgcgcaagg aatcctgcgt acgggagtaa ggtcgtctgg gtggaatacg aactggacgc
    15061 acagaaagtc agatatcgca tcacggatca gggccgcggc tttgaccaca aatcatttac
    15121 aaaacaacag atgcctgatg tgcgcagacc gcacggtcgc gggattctaa ttacaagcga
    15181 atgctttgat agtgtgactt acaatgaaac tggaaactcc gttgagttga ttcgcttttt
    15241 tccaactgca tagaaagact cgcttcgccc acggaccatc cgcgggcaaa gcaaaacatc
    15301 aggaatcagt gatgctcaga atgatctggt gccgggcgat cttgacccgg acccgaacga
    15361 tgatccatca taggatgacc ggggcccatc atctcacatc cgtttccatt cttgcgcatt
    15421 tccatcatct gttgcatatg cgcttgcatt tctttacgct cagtcagaag ttcagtgagt
    15481 atgtccgaca ttacatctac tttcttttga ccggttgcag tcttcagagt tgcgagcatc
    15541 ttttcaagct tcgcatcccg agccttgcga ccttccatca tcttttcatg cattgccttg
    15601 cattcaggac tcggctttct ttcagggggt ggagtctgcg caaataaagg caacgaaagt
    15661 gtaagagaaa ccagggccag agataccaat agattctttt tcatacaccc agttagacga
    15721 tcagcaatgg atcgcaccta cattcctgcc tcacgtcttg cttctaaaag atgatttttg
    15781 tcagcaaaag ccattggatg taaagcgaaa gtagcgcaaa cttttctgct attgaatggc
    15841 ttcgaggtag tagacccgga ttgcctctga tttacccttc gctttgagta agtacggtcg
    15901 ggaaactgtg actttacccc tcactctttc atagagtacg gcagaaatca tgactctccc
    15961 cggttcacat gcatgttcca gtctgctcgc tacattcacg gcatctccaa tcaaagtgtt
    16021 ttccttccga atggtgctgc ccacatttcc gcggatggct ctcccggaat tcataccaat
    16081 tcgcgcgccc agttccgctt ttccggaagc gcgtctgttc gcattgatct tcgcgatcgc
    16141 atccctggag cgaatcgcag cgcgagcggc tgacagtgga tctgaaaatg tgccaaagat
    16201 agaatctccg ataaacttat ccacatctcc gccttcttcg tagatgatgt ccgtgactgg
    16261 agcaagaatt tcattcaaag aatggatcac ctcttgcgct ggatgagtat cactgaagcg
    16321 tgtaaacgaa cgaatatcga gaaagaaata cgtcatgtcg atttcttcat ctgcaattcc
    16381 gatcattcct tctcctgcag caaggtccgc tttttcccag gtgctccgtg gagtaaactg
    16441 gcggatgacc tcttgcaaca tcaaggacct tgtattgagt atgatgtttt cttcgttgcg
    16501 acgctccagc agtcgccgtt gctccagcat tctttgcttc tgtaaataca gctcaatgtg
    16561 cgtttcaatt cgaacgatga gttcttgctc atgcaccggt tttgtgatgt aatcgtttgc
    16621 gcctgcagtg agtgcctgga ccactctctt cttgtcttcg tccgcggata aaaagataat
    16681 gggagtctct ctcatagatg catgctcacg aatcttcatg catgtctgga ttccggacat
    16741 acctggaaga ctgaagtcga gcagaatgca gtcaggatgg agatctggta gatgctgcaa
    16801 ggcttcttct gcgttgttga atgttactgg ttgaatccct gcatgtccaa ggtagtgtcg
    16861 gatcagatcc agtatggcag gatcatcatc cacagtcaca acaatcgatt cgcggcgagt
    16921 caggatgtca ttttcagatt ctgccatggc gcacgaatgg aacagcaatg cgccgccgct
    16981 agcattttat cgtcaggtga agtgagtcaa agtcagcaag aaaggccagt gcccagggtg
    17041 gccctgaatt tcctggagcg cagctttgag aatactctgc cttgataagg tgcagtcatg
    17101 cagcgttcta gctcagccgt tggaggggcc ccgcatacat tctctttgta cttttccata
    17161 gaaatgattc gcccgtcttc attcgtagtg aacacaaact tgatgtagat gctggcacat
    17221 ccgccgcagg acatacgcaa accggcgctt ttcatacatg gaccatacgt ttctttgaac
    17281 caccggtccc gtgccaggga ccattcgcga tctcccgaat attcaccggg aagagtgtcg
    17341 gggtttgcct gaacaaccgg cgaatccaca ggcgcgcttt cgcgcgctcg atggcatgct
    17401 tccaggccaa atagaaccac gaccagtaga actaaccctg aaagacg
//
LOCUS       DAOQJL010000007        17433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1542_length_17433_cov_4.659685, whole genome shotgun sequence.
ACCESSION   DAOQJL010000007 DAOQJL010000000
VERSION     DAOQJL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_17433_cov_4.659685"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLG78_00580"
     CDS             <1..981
                     /locus_tag="PLG78_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21628.1"
                     /translation="DIEALRLFCMRMATILQITRDPLTSRDVASTGKYREDIAYQWST
                     VFTENLEALIHPTGPDWDSMGIATARAVTNAPPQAPPVGAAAYRLNAGGTQSWNNSAY
                     YYFTYDPKLMEGERARLTNIVHIDTHMGADQNVMRSERILKYTRKVAHPDVHENANVE
                     LEYTGFLHSFFNLVIEISTLNMGIPQKTRDLFLFHLGPQSFYYLTRRFLQELDTGTIH
                     RKSGRGTSMEKFVPGELLKKIILEWWQAHILNVVGMERDDLSQYRMICKLVKDTYDRL
                     SAKAMAEYDQLPENEKVGKNRAEIIRENLNKWLGPTNIIVFRRFLKSGTP"
     gene            complement(941..2725)
                     /locus_tag="PLG78_00585"
     CDS             complement(941..2725)
                     /locus_tag="PLG78_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21629.1"
                     /translation="MESLSSILKKASIPFSLGLIAFGLLVGGWKIQRDWTEFAACPNR
                     DVLTWDANLRMLQTLDVRDDFVHGKSFRAIGNILESPTWPPLHGLIALVAYLFTSPST
                     VAEAGIGMLFFALTIALLAAMAAYIGKSEDMMISASLSFVASVIILSHTRELAAYATS
                     AMLETQGMFFLALTMLALYRSFRNGKGITLLTFSALGLFLTKYPYGHILVMAVVPVAI
                     AAHFESIGPMISFLRKRYRGAALILPGALMLLIAALAMARFLPEGILKVKGFKYVFYG
                     LVVLLWVDLNWKFYQARAELNWIPPVWKTIYISVVTPILFLTFLNPDRFSSTVGTQLH
                     EQDSARSFLLSFFDLTDSPVPLFAFAGLLVVSIVFAAIKRKSKLTGDPLFAMLLVLIM
                     HVLILEFLTANKQLRHLYHLLPAILLSGMVFALREIRYPIRIIAGFILLGSASILIAS
                     KGSVLKAEHYMFRYHCWTGVEANMYEPVRTLVSQLPAQKRYMLINRFHEPDAPLPGRL
                     YATDFDLLIRMKPGTSARNDSRYQWKSWSEFDSVLLLQRECNDPVGENLLFARAQTVG
                     SVVNPGKQWTVPLLCIKEYPISKNGGTQ"
     gene            complement(2701..3261)
                     /locus_tag="PLG78_00590"
     CDS             complement(2701..3261)
                     /locus_tag="PLG78_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21630.1"
                     /translation="MIYKKLILIVSLLALFSTECRTVRPRDMIPGLERGRVIYSEGAR
                     KPTEAELTAVDLLLKQLLSMVQRKDWSDLPGLVSKKKGIYVDLKGFRSFAQLEADVRD
                     RGSYLYVFYHDTDTLRAFTHDKDQIAVREVLMSGNSIRVDYYMEEGSQECELALHATG
                     NEKEDYRLNHPVFIREDGVWKVYRLF"
     gene            complement(3258..4817)
                     /locus_tag="PLG78_00595"
     CDS             complement(3258..4817)
                     /locus_tag="PLG78_00595"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNE21631.1"
                     /translation="MVIVSVIIGLAIAGVAAYYLLFTDRRDVYERALNMAAEGHFVDA
                     RGLIRAKIEGHPDNSIGHYTMARVYALEGKTEDEKRHLLEVRRINAYGADVHPVSVLN
                     RIGDIEYSDGDYRASVDAYLATLQFSPQNEEALARVAFLTVGRSKFDIAERYFRTLVR
                     IAPNTLEYRIGRGVGLAMLKSKDAIREFETAAALAPNDPTPTFFLAFQHYRQREFDRA
                     REAIERTLPLVMDPIVLHLANRLATVIYFHVKDYPRAISCANQCLATAIESDLKREEY
                     DARLSLAILGIASSNLELANDQLLELELVNPSDLTVQKLADFRMDLEEGSAALDRISP
                     RGFDFNAQIQDWLRNRFPEDMIYRLSGLEMEDEFNIDDYFTSEGEVQKKKVESTIDPA
                     LMIERFNTLTDVPFQNACAKIISLQGFQLEKTLPYREKDGGDFVAANKNDKKVKALFR
                     FRKWKNQPISDIYLREMQNLMNEMKMNQGFFVAGTRLTPGAESALANLKKITVLHDIE
                     LAEILFKVLGQ"
     gene            complement(4819..5559)
                     /locus_tag="PLG78_00600"
     CDS             complement(4819..5559)
                     /locus_tag="PLG78_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21632.1"
                     /translation="MKLLLLTGIQEELYPMIPEFRLVFSKETGLYQSANYPELFAATT
                     GPGVKKRKELREIFKTIIPDIIVNAGLIGTLTDDPLPAPGDLAKISSVVEFETHLVFP
                     VQPHGGTLLTVRKPVFEPREKWLLSRDYQARFCDMEASRILQLLREVDEVREDTSVVF
                     CKVVGDTPAQYDLYQHEELVRGWHRENWFGKMRIGLKFPGGPMRLRRLLALKEKSLDG
                     LTEHTRRTVKALLAGANPDKMGAVYAPA"
     gene            5652..6797
                     /locus_tag="PLG78_00605"
     CDS             5652..6797
                     /locus_tag="PLG78_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE21633.1"
                     /translation="MDFSLSKELVDFRESVRQFAIQHVKPTAEQRDLDERWDPDIWRK
                     MGEFGLLGLCFPEEYGGSGADCLTTTVATEAFAEGATDGGLTLAWGAHTIIGTMPIVL
                     CGTKEQKERYVPKLASGEWVAGLGLTEPGSGSDAAGSMETRAEKKGDRYILNGAKMFI
                     TNGPIGDVFTVMAVTDKKKGPLGISVFLVEKNFPGFRVGKKLKKMGMRSSTTSELIFE
                     DCEVPAENMIEKPNSGFLRVGKATLEWERTVLVASAIGSMHHMLDESIRYAKARRQFG
                     EPIARFQAIQEKISRVRMKLDASRLLLYKSAVMKDQGKPAPIESSLAKVHATEAVIEA
                     AYEMGQIHGGYCFIHEYPVERAYRDCRLGTLGGGTSEVMRSIIAANL"
     gene            6826..8040
                     /locus_tag="PLG78_00610"
     CDS             6826..8040
                     /locus_tag="PLG78_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE21634.1"
                     /translation="MDFELNQEDKEFAEHFRRFCEKEIGAVAGEVDESGSVPRSHYEA
                     LGAIGYNGLLHRREFGGQEASIVQATVAQMILAEACGSTFFSVGASCGLFGGPVASYA
                     SAAQKKSILPTLLAGKLIGCLAVTEPDTGSDVSAIRTRAKRSANGFVLNGQKTFITNA
                     PIGDYAVVLARLGEENADSKGSLSCFLVDLHLPGVTRGKAMRKMGLRASPTGELFFDN
                     VELAAESLLGAPGQGFRMVMEAFAMERLALAAYCVGTMKACFDDSRRYSRERKAFGRP
                     IAKHQSVAFMLADIKTKLEAAYLLLMETAWLVDRQRAEAVGGKSPSRLMHNGYVIDIN
                     ARAAETKLLASTYAREVTNLAVQVHGGAGYMEEYRVCRLYRDIKIAEIGGGTSEIQKQ
                     IVAHAEIKRVGV"
     gene            8040..9053
                     /locus_tag="PLG78_00615"
     CDS             8040..9053
                     /locus_tag="PLG78_00615"
                     /inference="COORDINATES: protein motif:HMM:NF028249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNE21635.1"
                     /translation="MIRTCAFAIWTSLIFASAAHLSAETSITVSMDRTWNDGETVFEI
                     GSKQPDITGIAGGSRITYPRDFAYTRMALGFYSGKVGIDLDVGATGHAVRSGTGRDED
                     FFLYSTSRVDGAKVSLQDGRFSDRTRVFSGGRNWADSSGKTALVEYRARLITNYFPWS
                     DASPFHRDSGTFFALGFEHIYSKYRIYDSIQYNVVSVTPTNPYPFNLTPIGSGLSFTN
                     VSGELMLGAGVALYPWEAVGFSFSLSPLVGYANSRDYHSLRGITFIMENAGSGFLYQM
                     ETMIFPADRFLVRLGVTGHRFYASGTVKAHGLDLLYNVLPPQQMFLNTKEWGAKISLE
                     YLL"
     gene            complement(9055..9858)
                     /locus_tag="PLG78_00620"
     CDS             complement(9055..9858)
                     /locus_tag="PLG78_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21636.1"
                     /translation="MKFGELKEDFRKRWPEWSARIIQTVRELPSTIRQAAKDSWPVIQ
                     SEARALPARLKAVPGHVVGFFAKLASVDGGPLRKLTFLWCFALGFSAVCILGRTNPFA
                     LLIPVWGVTRPASDSRSAVSLRMFPAEGDAIISVTRKLRLNDSLEHNLEALARTVSET
                     AGIHDTSAPVANLPDYAFAIRKVWVTKEKSCILDFRSETLRVETEQYTRNRTKIGEKA
                     VLLDRYFQSLTESIFASAIGCKSVRYLLDGKVDQVKEMKFDLKVERTPG"
     gene            9910..10653
                     /locus_tag="PLG78_00625"
     CDS             9910..10653
                     /locus_tag="PLG78_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21637.1"
                     /translation="MSVEKSVKEIRATGLLVVFAALCTALSAGIITAVAGSPKPNPGS
                     AFQNAIVEFELAVNSREVQAVLSQPTEELARARRTQFNYLNYADYLFMVCYSALNATI
                     FLYVAALNRSRFRERFVGNAFIRFGLLLSFLMLAGDALENVQLLRLTSMPEPEMSSTI
                     KALIIFTRIKWGALFTAAILLGLAYASYSGRTGLLFVGFLYATAGSIGIMSLIVLSGR
                     PLCEWGTLLLAVAWLVSAVHAGVRTFRRA"
     gene            10702..10774
                     /locus_tag="PLG78_00630"
     tRNA            10702..10774
                     /locus_tag="PLG78_00630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10735..10737,aa:Thr,seq:tgt)
     gene            10778..10860
                     /locus_tag="PLG78_00635"
     tRNA            10778..10860
                     /locus_tag="PLG78_00635"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10812..10814,aa:Tyr,seq:gta)
     gene            10910..11974
                     /locus_tag="PLG78_00640"
     CDS             10910..11974
                     /locus_tag="PLG78_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE21638.1"
                     /translation="MLGIRYIKTRPTEYVMLFRGGKLRKHGAGLAFFYYAPSSSVVIL
                     PAESRDIPFIFNEVSNDFQEITIQGQATYRVRDPEKLAALLDFSVDVFSNYTGDGAEK
                     LGVRVTNSVQLAIREKFKEMSLRDALAGASALVQHAAGKLKSSELLSALGIEILDLSI
                     LKIAPTPDMSRALEASAREKLYKEADDAIYERRNSAVEQERRIKENELQTQIAIEEKN
                     RKIREEQMNAEIAVQEKQRAVEEEKMKTVESVERKKAEIEDQKMLSRTALEEKKRKLV
                     EVEVQNNIQYAKARAESMRLELEAVMKLPPDVLEVLMANQMQSRKLISRAMRDLAKNA
                     QKIGTLNITPDLLQNLLKDE"
     gene            11971..12903
                     /locus_tag="PLG78_00645"
     CDS             11971..12903
                     /locus_tag="PLG78_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HNE21639.1"
                     /translation="MTTVEKAVVVTRPTRLQESVKRYNTKAQAKFYVEKRGQSFQDYE
                     LEEDIYEHSRDHVLKAIPRDLKIQIVDRNFLPNYLFSPKDLIICLGQDGLVVNTAKYL
                     EGQPVIAINPDPDRFDGVLLPFLPDQTEAAVQAVMNGKFQARNLTMARVDMNDGQKLY
                     AFNDLFIGPRSHITARYTIHDGERSERQMSSGILVATPAGSTGWISSLFNQTRGIARF
                     QGDGSPLAGMRLEWEDQKLVYMVREPFASKWSKTEMVAGVIEGTNSISVESHMSESGV
                     IFSDGMESDFIQFNTGSVARIGIAEKSTTLVVKF"
     gene            complement(12921..13838)
                     /locus_tag="PLG78_00650"
     CDS             complement(12921..13838)
                     /locus_tag="PLG78_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21640.1"
                     /translation="MRPQPFLLAIGYLLLAVPVMGEPWTQFKVKKAFISPETYQENKD
                     TFSDPEFGWNVFCENDLIMRAGLYDLLFSPPCKGKIVLYFVERDVVWDDKITPFPVAL
                     QPEKQRLVDGEDEFEIQLSSYNPDSNSGPDSITPRILKDGEVVKDHVSFLDGDRTDTF
                     ACPAGITLLMRSSLSVQTEASALWTDDSQKLSRIDSKDNVRILSPPGFAVSDYTIACA
                     GNTSIMLQTLSKLASEDKGLFRSARQLIDSNAEAQKYCNSNLLEPVCIYRQYMSQKGD
                     RIRLENMRLPPAARAACNQAREDATYLEN"
     gene            complement(13835..15079)
                     /locus_tag="PLG78_00655"
     CDS             complement(13835..15079)
                     /locus_tag="PLG78_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNE21641.1"
                     /translation="MQDHLFSPAAPLPQRLRPSSFEEFHGQQSLIDQLAGKKPHSMIL
                     YGPPGCGKTTLALLLAKKATLPFRSLSAVASGVKELRDVIEEARKTGRILLFVDEIHR
                     FSKSQQDALLHAVEEGWLVLIGATTENPSFEVISPLLSRCQVYKLGALSSEDLDRILE
                     RALMQDSLLKGKTIEGEAREALIQFAGGDARKLLRVVEIAAEEPDIITLEALNKSIQN
                     FARQYDRAGENHYDVISAFIKSMRGSDPDAALLYLAIMIDAGADPLFIARRIIIFASE
                     DVGNAAPQGLQVAVSAFQALERVGMPEGRIILAQAATFLASCPKSNAAYRGIDAALEF
                     IQGKRISVPNHLRNAPTTTHRKEGAGKGYKYPHEYSGHYVEQEYMPDDIRAQFYFPTR
                     EGQEVRLLERLRMLSRKDRKYE"
     gene            15159..15578
                     /locus_tag="PLG78_00660"
     CDS             15159..15578
                     /locus_tag="PLG78_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21642.1"
                     /translation="MSQSEQKETIEELAASLEKISQKKLKYDIVRHWFQGREPYFDLF
                     VNTLNDTLVWFQFTFRGHSICWSQQDPIIRTGVTTDFALQDVSYYSATKTVKLDEEID
                     PQFLAFCKKLAGCRATEEPFRSILKVLSSPPDLTANQ"
     gene            complement(15572..16246)
                     /gene="nth"
                     /locus_tag="PLG78_00665"
     CDS             complement(15572..16246)
                     /gene="nth"
                     /locus_tag="PLG78_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNE21643.1"
                     /translation="MDFEITGDAALKQRDDAEEFVKNAYKLLQKKFGDVKCPLTYGKP
                     HELCIAVILSAQCTDAQVNKTTPALFARFPNPGDYISAPLSEVENLVHSTGFYRNKAK
                     NIQGFCHSLVHEHGGVVPRTLAELTAMPGVGRKTANVVLQELYGIPSGMVVDTHVARL
                     SRIFGLTKSKNAVQIERDLMQIVPERMWMNWSLYMIFLGRSNCTARTRDCANCILKKI
                     CPTANY"
     gene            complement(16213..17241)
                     /locus_tag="PLG78_00670"
     CDS             complement(16213..17241)
                     /locus_tag="PLG78_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE21644.1"
                     /translation="MRFAVTGSHRFDRKTAVLIAYGGFLVGTLLCALSQEYSQLMAAR
                     VLAGAFGGVLGALVLSMVSDAVPESRRGQAVGIIMASFSVATIAGVPAGLLLNEVLNW
                     HVPFYFITAIGAIIWPIAFLHLPAFRGHIAPVKRSALETLKSMGSMITHGSHLRAMVL
                     TVLLMFSGFTVIPYIAPYLTYNVGLEKKYLALVYLCGGLATIFSSRYAGRLADRHGKQ
                     KVFSIIGLFSIIPIVALTSLPPLPMAAILGVTTIFMIFVNGRFVPAMAMITTSARPKD
                     RGSFMSVQSSVQHLFSGVSAAIGGALLTKGPEHTLIHYEWVGYFASAMTIVAIAWVWT
                     LKSQETPR"
BASE COUNT         4304 a         4320 c         4508 g         4301 t
ORIGIN      
        1 gacattgaag ctttgcgtct gttctgcatg cgcatggcca cgatccttca gatcactcga
       61 gatcccctga catcgcgcga cgttgcgagc acaggaaagt atcgtgagga cattgcctat
      121 cagtggtcca ccgtattcac cgagaatctg gaagcgctca tccatcccac cggtcctgac
      181 tgggattcca tgggaatcgc cacagctcgc gcggttacga atgcacctcc gcaggcgcct
      241 cctgttggag ccgccgctta tcgtctgaac gcgggtggca cccagagctg gaacaattct
      301 gcgtattact actttaccta tgatccgaag ctcatggagg gagagagagc gcgccttacg
      361 aatattgtgc acattgacac tcacatgggt gcggatcaga acgttatgcg atcagagagg
      421 attctgaagt atacgcgcaa agtggctcat ccggacgtgc acgaaaacgc gaacgtggag
      481 ttggagtaca ctggttttct tcacagcttc tttaatctcg taatcgaaat cagtaccctc
      541 aatatgggca ttccgcaaaa aactcgagat ctgtttttgt ttcacctcgg gccccagtca
      601 ttctattatc tcactcgtcg ttttcttcag gagcttgata cagggaccat tcaccgcaag
      661 tccggacgcg gaacaagcat ggagaagttt gttccaggtg aattgctaaa aaagatcatc
      721 ctcgaatggt ggcaggcgca tatcctcaac gtagttggaa tggagcgaga cgatctttct
      781 cagtacagaa tgatttgcaa attagtaaaa gatacttatg atcgtctgtc tgcaaaagca
      841 atggctgaat atgatcagtt gcccgagaac gaaaaggtcg gtaagaatcg cgcagaaatc
      901 attcgcgaaa acctgaacaa gtggttgggc ccaacgaata tcattgtgtt ccgccgtttt
      961 ttgaaatcgg gtactcctta atacaaagga gaggcacagt ccactgcttg cctggattga
     1021 ctacactacc cactgtctga gcccgcgcaa atagcaagtt ttcaccaact ggatcgttgc
     1081 attctctttg cagcaagagc acgctgtcaa attcgctcca ggatttccac tggtagcgtg
     1141 aatcgtttcg agcgcttgtg ccgggtttca tccgaattaa gagatcgaag tcggtcgcgt
     1201 agagccttcc cggaagaggt gcgtccggtt catgaaagcg attgatgagc atgtatctct
     1261 tttgtgctgg aagttggctt acaagtgtgc gaacaggttc atacatgttc gcttccacgc
     1321 cggtccagca atgatagcga aacatataat gctctgcctt taagacactt ccttttgaag
     1381 ctatcaaaat ggatgcgctc ccaagcaaaa tgaatccagc gatgatgcgg atcggatacc
     1441 ggatttccct gagtgcaaag accatcccac tgagaaggat cgcggggagc agatggtata
     1501 agtgacgcag ctgcttgttc gcagttagaa attcgaggat caacacgtgc atgatgagca
     1561 cgaggagcat tgcgaacaat ggatctccag taagtttgga ctttcttttg atcgcggcaa
     1621 atacgatcga cacaacaagc aagccggcaa aagcgaataa aggcaccgga gaatctgtaa
     1681 gatcgaagaa actcagtaaa aagcttcgtg cggagtcctg ttcgtgcaac tgtgtcccta
     1741 cggtgctcga aaaccggtcg gggttcagga atgtcagaaa gagaatgggt gtaacaacag
     1801 agatatagat cgtcttccag acggggggaa tccagttcaa ctccgcgcgc gcctgataga
     1861 atttccaatt gaggtcgacc cagagcaaga ccacgagtcc gtaaaagaca tacttgaagc
     1921 ctttcacttt tagaattcct tccggaagaa aacgcgccat ggcgagagct gcgatcagaa
     1981 gcataagagc gccgggcaga attagcgccg caccccgata gcgttttctg agaaaggaga
     2041 tcatcgggcc aatcgattca aaatgcgcag cgattgctac aggaacgact gccattacaa
     2101 ggatatggcc ataaggatac ttcgtgagaa agaggccaag ggcactgaac gtcagcagtg
     2161 tgattccttt gccgttgcga aaactcctgt aaagtgcgag catcgtcagt gctagaaaga
     2221 acatgccctg agtctcgagc atggcggaag tagcataggc ggcgagttcc cgcgtatgac
     2281 tgagaatgat cacactcgcc acaaagctca atgaagcgga gatcatcatg tcctctgact
     2341 ttcctatgta cgcagccata gctgcgagca aggcgatggt aagagcaaag aataacatcc
     2401 cgatgcctgc ttccgccact gtagacggag acgtgaacaa gtaagcgacc agggcgatca
     2461 agccgtgaag gggaggccag gtaggggatt ccagaatgtt gccaatggct cgaaatgatt
     2521 ttccgtgcac aaagtcatcg cgaacatcca gagtctggag cattctcaga ttggcgtccc
     2581 acgtgagcac atccctgttc gggcaagctg caaattctgt ccaatccctt tgaatcttcc
     2641 atcctccaac taacagccca aacgcgatca agcccagaga gaacggaatc gatgcctttt
     2701 tcagaataga cgatagactt tccaaactcc gtcctcccga atgaacacag gatgattcaa
     2761 gcggtagtct tctttctcat ttccggtagc gtgcagagca agttcgcatt cctgactgcc
     2821 ttcttccata tagtaatcca cgcgaataga gtttcctgac atgagcacct ctcgcacggc
     2881 aatctggtct ttgtcatgag tgaaggctcg cagtgtgtct gtatcatgat agaacacgta
     2941 caggtagcta ccgcgatctc ggacgtcggc ttccaattgc gcaaacgatc taaagccttt
     3001 tagatccacg tatataccct tcttcttgct gaccagtcca ggcagatcgc tccagtcctt
     3061 tctctgcacc atggacagaa gttgtttgag tagcaaatct accgctgtaa gttccgcttc
     3121 cgttggcttt cgcgcaccct ctgaatagat aacgcgtccc ctctcgagcc cggggatcat
     3181 gtcgcgcggg cgaaccgttc ggcactcagt cgagaataga gcaagaagcg agactatgag
     3241 tatgagcttt ttgtaaatca ctggcccagg accttgaaga ggatctctgc cagttcaatg
     3301 tcgtggagaa cagtaatttt tttcagattc gcgagcgcgg attctgcacc tggagtcagg
     3361 cgcgttcctg ccacaaagaa gccttgattc attttcatct cgttcatcag gttctgcatt
     3421 tctctcaaat agatatcaga gatgggctgg ttcttccact ttctaaagcg aaagagcgct
     3481 ttcacctttt tgtcattctt gtttgccgcg acaaagtctc cgccatcctt ttcgcggtaa
     3541 ggcaaagtct tctcgagctg aaatccctgg agagaaatga tcttagcgca tgcattttga
     3601 aagggaacgt ccgttagagt gttgaatcgc tcgatcatca acgccggatc tatggtcgat
     3661 tccaccttct tcttttggac ttccccttcg gaagtgaaat agtcgtctat gttgaattcg
     3721 tcttccatct cgaggccgga cagtcgatag atcatatctt ccgggaatcg attgcgaagc
     3781 cagtcctgaa tttgtgcatt aaaatcaaag ccgcggggac tgattcgatc gagcgcggca
     3841 ctgccctctt ctaaatccat gcgaaagtca gccagctttt gcactgtcag gtcggacggg
     3901 tttacaagct caagttcaag caactgatca ttggcgagtt ctagattgct cgaagcgatc
     3961 ccgagaatgg caagactcaa gcgtgcgtca tactcttccc tttttagatc tgactctatg
     4021 gctgtggcca gacactggtt cgcgcacgat atggctcgcg gataatcttt cacatgaaag
     4081 tagattacgg tggccaaacg attcgccagg tgcaaaacaa ttgggtccat gaccagaggt
     4141 agagttctct caatcgcctc ccgagctcta tcgaattccc tttgtctata gtgttgaaat
     4201 gctagaaaga atgttggggt gggatcattt ggtgccagag ctgcggcagt ttcgaattcg
     4261 cggatcgcat ccttgctttt gagcatcgcc agtcccactc cgcgtcctat gcgatattcc
     4321 agagtgttag gtgctatgcg aacaagagtg cgaaagtatc tttccgcaat atcaaacttg
     4381 gatctgccca cagtcaggaa tgctacgcgt gccagggcct cttcgttttg tggtgagaat
     4441 tgcagtgtgg ccagataagc gtctacactg gcgcgatagt ctccgtctga atattcaata
     4501 tccccgattc gattcagaac actgaccgga tgcacatcgg ctccgtatgc gttgattcgc
     4561 cgcacttcta ggagatgtct tttctcatcc tctgtctttc cctcgagtgc gtaaacccgg
     4621 gccattgtgt agtggccgat ggaattatcc ggatgtcctt caatcttcgc gcgaatcaaa
     4681 ccacgcgcgt ctacgaaatg accttcggct gccatgttga gagcgcgctc gtacacgtcg
     4741 cggcgatcgg tgaacaacaa atagtatgct gcaacacctg cgatggcaag accaatgatg
     4801 acagatacga tgaccatact aagccggcgc gtacactgcg cccattttgt ctggattggc
     4861 tccggcgaga agtgctttca cggttctgcg cgtatgctcc gtcagaccat ccagggattt
     4921 ttctttcaag gccagcaatc tgcggagccg catgggcccg cctggaaatt ttaaaccaat
     4981 ccgcatcttc ccgaaccagt tctctctgtg ccatccccgc acgagttctt catgttgata
     5041 gaggtcatat tgggccggtg tatccccgac gaccttgcaa aatacaacag aggtgtcctc
     5101 acgcacttca tccacttctc gaagaagttg caggattctg gatgcctcca tatcacagaa
     5161 gcgagcttga tagtcgcggg aaagcaacca tttctctctg ggttcaaaga ccggtttacg
     5221 aacggtcagg agtgttcctc cgtgtggctg caccggaaac accaggtgcg tttcaaattc
     5281 aacaaccgat gagatcttcg cgagatcccc cggagccggc agaggatcat ccgtgagagt
     5341 tccaataagc cccgcgttca cgatgatgtc cggtatgatc gttttgaaga tttcgcgtag
     5401 ctctttgcgc tttttgacac cgggtcccgt ggtcgcggca aacagttcgg ggtagttggc
     5461 cgattggtag aggccggtct ctttcgaaaa caccaggcga aactcaggaa tcatcggata
     5521 gagctcttcc tggatgcctg tgaggagtaa caatttcata tcggccggga tgtcttataa
     5581 tacggagatt ttccatctgc tctgcacaga aaaagcctgg cgtcacccgc taggaaaaag
     5641 caatcggtgt tatggatttt tctttgtcca aagagctcgt cgattttcgc gaatcggtgc
     5701 ggcaattcgc catccagcac gtgaagccca cagcggagca gcgcgacctg gacgagcgct
     5761 gggatcccga tatctggaga aaaatgggtg agttcggcct acttgggctg tgctttccag
     5821 aggaatatgg cggctcaggt gccgattgcc tgacaaccac agtggccaca gaggcattcg
     5881 cagaaggcgc cacggacgga ggactcactc ttgcctgggg tgcgcatacg atcattggca
     5941 ctatgccgat cgttctgtgc gggaccaaag aacaaaagga aagatacgtg cctaagctcg
     6001 ccagcggcga atgggtggcg ggcctgggtt tgacagaacc aggatccggt tcagacgccg
     6061 cgggaagcat ggagactcgt gcggaaaaaa agggggatcg ttatattctt aacggcgcca
     6121 agatgttcat cacaaacgga cccattggag atgtatttac cgtgatggcg gttacggaca
     6181 agaagaaagg accactgggg atctccgtct ttctcgtaga gaagaatttt ccaggctttc
     6241 gcgttgggaa gaaactcaaa aagatgggaa tgcgatcgag cacaaccagc gaacttattt
     6301 ttgaagattg tgaagtgccc gcagaaaaca tgatcgagaa acctaactcc ggatttctca
     6361 gagtgggcaa ggccactctg gaatgggagc gtacggttct cgtcgccagt gccatcggat
     6421 ctatgcatca tatgctcgat gaatcgatcc gatatgcgaa agcccgccgt caatttggtg
     6481 aacccatcgc gcgctttcag gcaattcaag aaaagatctc cagggttcgc atgaaactcg
     6541 atgcgtcaag attgcttcta tataagagtg cagtgatgaa agaccaggga aagcccgcgc
     6601 ccatcgaaag ttcactcgca aaagtacatg cgaccgaagc agtgattgaa gccgcctacg
     6661 agatgggaca aatccacggg ggctattgct ttattcatga atatccggtg gagagagcat
     6721 accgcgattg tagactgggc actctgggtg gcggaacttc tgaagtgatg agatctatca
     6781 tcgcggccaa cctgtgagac attcaaacgg aattgtggtg taattatgga ctttgaactg
     6841 aatcaagaag acaaagaatt tgcggagcat ttcagacgct tctgcgaaaa agagattggc
     6901 gctgtagccg gtgaagtgga tgaatctgga agtgtgccgc gttcgcatta cgaggcgtta
     6961 ggtgccattg gctacaatgg tctcctccat cgccgtgaat tcggtgggca agaagcttcc
     7021 attgtgcagg cgaccgttgc gcagatgatt ctcgcggaag cttgcggctc tactttcttc
     7081 tctgtgggtg cctcctgcgg cctcttcggt gggccagtgg cgtcctatgc atctgcagct
     7141 caaaagaaat ccattctgcc aaccttgctt gcgggaaaac tcattggatg cctcgcggtt
     7201 actgagccag acactggttc ggacgtaagt gcaattcgca cccgtgccaa acggtcggcc
     7261 aacggatttg ttttgaatgg gcaaaaaaca ttcattacga acgcgcccat cggggattac
     7321 gcagtggttc tggcgcgcct tggtgaggag aatgcggatt ctaagggatc cttgagctgc
     7381 tttctggtag accttcacct tcccggtgtt acgcgaggca aggctatgcg aaaaatgggg
     7441 ttgcgtgcct ctcccacagg agagttgttt tttgacaatg tggaacttgc ggccgaatcc
     7501 ctcctgggtg caccaggcca gggctttcgc atggtcatgg aagcgttcgc catggaacgg
     7561 cttgctttag cggcttattg cgttggtacc atgaaagctt gctttgacga ttcacgtcgc
     7621 tattcgcggg aaagaaaggc tttcggacgg cccattgcga aacatcaaag tgttgcattc
     7681 atgctggcag acatcaaaac aaagctggaa gcagcttatc ttttgctcat ggaaacagca
     7741 tggctcgtgg accgacagag ggcagaagca gtgggtggaa agtcaccgtc cagactcatg
     7801 cacaacggtt acgtgataga catcaatgct cgcgctgcgg aaacaaagct tcttgcttct
     7861 acttacgcgc gtgaggtgac caatctcgca gtgcaggttc acggaggtgc aggctacatg
     7921 gaagagtatc gggtgtgcag attgtatcgc gatatcaaga tcgcggaaat tggtggaggc
     7981 acttcagaga tacagaagca gatcgtcgct cacgcggaaa tcaagcgcgt cggtgtttga
     8041 tgattcgcac gtgcgcattt gcgatatgga cctcactcat cttcgcatct gccgcgcatc
     8101 tctctgcgga aacttccatc accgtttcca tggaccgcac gtggaatgac ggcgaaactg
     8161 tttttgaaat tgggtctaag caaccggaca tcactggaat cgctggcggt tctcgcatca
     8221 cgtatcccag agattttgca tacacgcgga tggctcttgg tttctactca gggaaagtcg
     8281 gcattgatct cgatgtagga gccacagggc acgcagtgcg atccggcaca ggtagagatg
     8341 aagacttctt tttgtattca acgtctcgtg ttgatggcgc caaagtctca ctgcaggatg
     8401 gacgattcag tgacaggact cgtgtttttt caggcggaag aaactgggcc gactcatctg
     8461 gcaagaccgc gctcgtcgag tatcgagcac gactgatcac aaactacttt ccgtggtctg
     8521 acgccagtcc ctttcaccgc gattcaggaa ctttttttgc actcggtttt gagcacatct
     8581 actccaagta tagaatctat gattctatac aatacaacgt agtgtccgtc actcccacca
     8641 atccatatcc ctttaattta accccgattg ggagtggtct ctcgtttaca aacgtaagcg
     8701 gagaactgat gctgggtgcg ggcgtggcgt tgtatccatg ggaggcagtc gggttttctt
     8761 tttctctttc gcctttggtt ggttatgcga acagccgcga ctatcattct ttgcgcggca
     8821 tcacattcat tatggagaat gctggatctg gttttctgta tcaaatggag acaatgattt
     8881 ttcccgctga ccgttttctt gtgcgattgg gtgtgaccgg gcataggttc tatgcaagtg
     8941 gaaccgtgaa ggcgcatgga cttgatttgc tgtacaatgt gctgccaccg cagcagatgt
     9001 ttttgaacac gaaggaatgg ggtgcgaaga tctctttgga atatctgctt tagatcagcc
     9061 cggtgttctt tccactttca aatcaaattt catttctttg acctggtcta ctttgccatc
     9121 caaaagatag cgcacacttt tgcatccgat tgcggatgcg aagatagatt ccgtgagcga
     9181 ttgaaaataa cggtccagca atactgcctt ttcgcctatc tttgtacgat tgcgagtgta
     9241 ctgttctgtt tctacgcgaa gagtttccga acgaaagtcc agaatgcaac ttttctcttt
     9301 cgtgacccat actttcctga tcgcgaatgc gtaatcggga aggttggcaa ccggagcaga
     9361 agtgtcgtga attcctgccg tttccgaaac tgttcgtgcg agtgcttcta gattgtgttc
     9421 cagcgaatcg ttcaggcgaa gcttccgcgt aaccgaaatg atggcatccc cttctgctgg
     9481 aaacatgcgc agtgaaaccg cactgcggga gtcggaagca ggcctggtga ctccccagac
     9541 tggaattaga agagcgaaag gatttgtgcg cccgagaatg cagactgcgc tgaaacccag
     9601 ggcaaagcac cagagaaagg ttagcttgcg caatggcccg ccatctacgg aggccagttt
     9661 agcaaagaaa cccaccacgt gaccgggaac ggctttgagc ctggcaggga gggctcgcgc
     9721 ctccgattga ataacaggcc acgaatcctt cgccgcctgg cgaatggtag agggcaattc
     9781 acggaccgtc tgaatgattc ttgcggacca ttctggccag cgtttgcgga aatcttcctt
     9841 gagttcgcca aacttcatgc tcttgtaaac tttattgtcc gcatttgtgc gtcgaccaaa
     9901 ttgcgtctca tgtccgtcga aaagtctgtc aaagaaatcc gcgctaccgg gctcctggtg
     9961 gtcttcgcag ccctgtgcac tgccctttct gccgggatca tcaccgccgt ggctggatcg
    10021 ccaaagccaa atcctggaag cgcattccag aatgccatcg ttgagtttga gctggctgtg
    10081 aattcgcggg aagtgcaggc cgttcttagc cagccgactg aggaactggc acgagccaga
    10141 cgaactcagt tcaactacct aaattacgca gactatctgt tcatggtatg ctacagtgct
    10201 ctgaatgcca cgatctttct gtatgtggcg gcgctcaatc gttcgcgttt cagagagcgg
    10261 tttgtaggaa acgcattcat ccgatttgga ctgcttttgt ctttcctgat gctcgcaggt
    10321 gatgcgcttg agaacgttca acttctaaga ctcacttcta tgcccgaacc tgagatgagt
    10381 tccacgatca aggcattgat cattttcacg cgcatcaagt ggggcgccct atttactgcc
    10441 gcgatcttgt tgggcctcgc gtatgcttct tactccggaa ggacgggttt gcttttcgta
    10501 gggtttcttt acgcgaccgc cgggtccatc ggcattatgt ccctgattgt tttgagcgga
    10561 aggccgctat gcgaatgggg aaccttgctg cttgccgtgg cctggctggt gtccgcagtg
    10621 cacgcaggtg tcagaacgtt tcgccgcgct tagtaccatt tgcttgaccc tgggggcctg
    10681 attgcatccc ttggtttcta cgctgcctta gctcagctgg tagagcaact gatttgtaat
    10741 cagtaggtcg tgggttcgaa gcctacaggc agcatatggg cagattcccg agtggctaaa
    10801 gggagcagac tgtaaatctg gcggcattcg ccttcggagg ttcgaatcct cctctgccca
    10861 gattccaaaa cgggtttact ttttttccac atgaagctat aagagccgaa tgcttggaat
    10921 tcgctacatc aagactcgcc ccacagaata cgttatgctc tttcgcggtg gtaagctgcg
    10981 aaaacacgga gctggtcttg ccttcttcta ctacgcgcct tcttccagtg ttgtgattct
    11041 tccggcagag agccgtgata ttccgttcat tttcaatgaa gtctcaaacg acttccagga
    11101 aatcacaatc cagggtcagg ctacctatcg cgtacgcgat ccagaaaagc tcgcagcact
    11161 tcttgacttt tccgtggatg tgttttcgaa ttatacaggc gacggagcag agaagcttgg
    11221 cgtgcgcgtt acgaattccg ttcagctggc cattcgcgaa aaatttaaag agatgagtct
    11281 gcgcgatgcc cttgcaggtg cgtccgcgct cgttcaacac gctgcgggta agctcaagtc
    11341 cagtgaactt ctgtcagccc ttggaattga aatacttgat ctttctatac taaagatcgc
    11401 gcccactcct gatatgtcca gggccctgga agcctccgcg cgcgaaaagc tctacaaaga
    11461 ggcagacgat gcgatttatg aacgtcgcaa ctcagcggtg gagcaagaac gccggattaa
    11521 ggaaaacgaa cttcaaactc agattgctat cgaagaaaag aatcgcaaga ttcgagaaga
    11581 acagatgaac gctgagattg cggtacagga aaaacagcgc gccgtagaag aagaaaagat
    11641 gaagaccgtg gaatccgtgg aaagaaaaaa agcggagatt gaagaccaga agatgctatc
    11701 gcgcactgca ctcgaggaaa agaagcgcaa gctcgtggaa gtggaagtgc aaaacaacat
    11761 tcaatacgcg aaagctcgtg ccgaatcaat gcgactagaa ctcgaagcag tgatgaagct
    11821 gccgccagat gttcttgagg tattgatggc caatcaaatg caatcgcgca aactgatcag
    11881 tcgcgccatg cgcgatcttg ccaaaaatgc tcagaagatt ggcacattga acatcacgcc
    11941 ggatttactg cagaacttgc tcaaagacga atgaccactg ttgaaaaagc cgttgtagtc
    12001 acgcggccca cgaggctgca ggaatcagtc aagagataca acacaaaggc gcaggccaag
    12061 ttttacgtag aaaaacgcgg ccaatccttc caggactacg aactcgaaga agatatctat
    12121 gagcattcgc gagatcatgt actcaaggca attccgcggg atctaaaaat ccaaatcgtg
    12181 gatcgaaact tccttcccaa ctatcttttt tctccaaagg atttgatcat ttgtctggga
    12241 caggatgggc tggtggtcaa cacggcgaaa taccttgaag gccaacccgt gatcgccatc
    12301 aatccagacc ccgatcgctt tgatggagtt cttttaccct ttttgccgga tcagacggag
    12361 gcggcagtgc aggccgtcat gaatggaaag tttcaggcga gaaacctcac catggcccgc
    12421 gtagatatga acgacgggca gaagttgtac gcattcaacg atctttttat cgggcccagg
    12481 tcgcacataa cagctcgtta tacgattcat gatggtgaac gctcagaaag acaaatgtca
    12541 agcggcattc tcgtggccac tcctgcgggc agcacgggct ggatcagttc gctcttcaac
    12601 cagaccaggg gcattgctcg atttcaaggg gacggttccc ccctggcggg aatgcgtctg
    12661 gaatgggagg atcagaagct ggtctacatg gttcgtgaac ctttcgcaag caaatggagt
    12721 aagactgaaa tggtggccgg agtcattgag ggcacaaaca gcatcagtgt ggaaagtcac
    12781 atgtctgaat ccggtgttat cttctccgat ggaatggaat ctgatttcat tcaattcaac
    12841 acgggctccg tggcgcgcat tggcattgcg gaaaaatcca ccacgcttgt tgtgaagttc
    12901 tagtctggct tacgtattct tcaattctcg aggtaggttg cgtcttcgcg cgcttgattg
    12961 caggcagctc tcgctgcagg aggaagtcgc atattttcca ggcgaatccg atctcctttc
    13021 tgactcatgt attgtctgta aatacacacg ggctccagca gattggaatt gcagtatttt
    13081 tgagcctctg cgtttgaatc gatgagctgt ctggcagagc gaaacaaccc tttgtcttcc
    13141 gacgcaagtt tgctaagagt ctgaagcata atcgatgtat ttccagcgca tgcaatggtg
    13201 tagtcggaca ccgcaaatcc aggagggctc aaaatacgca cgttgtcctt cgagtctatt
    13261 cgggaaagtt tctgtgaatc gtctgtccag agagcggacg cttctgtctg aacagacaga
    13321 gagcttcgca taagcagtgt tatgccggca ggacaagcga atgtgtctgt gcgatcaccg
    13381 tccagaaaag acacatggtc tttgacgact tctccgtcct tgagaattcg cggagtgatt
    13441 gaatccgggc ctgaatttga atctggattg taagaggaga gttgaatttc gaattcgtct
    13501 tccccgtcaa cgagtcgttg tttctccggt tgcaaggcca ctggaaaggg cgtaattttg
    13561 tcatcccaga ccacgtccct ctcaacgaag tacagtacga tcttgccttt gcacggagga
    13621 ctgaagagca gatcgtagag acccgcccgc atgatgagat cattttcgca aaatacattc
    13681 catccgaatt caggatcact gaaagtatct ttattttcct ggtatgtttc cgggctaatg
    13741 aaagctttct tgactttgaa ctgagtccag ggttccccca tgacgggcac tgccaggaga
    13801 agatacccaa tggccaggag aaacggttgc ggcctcattc gtatttcctg tcctttcgcg
    13861 aaagcattcg cagtctctct aagagcctca cttcctggcc ttcccttgtt ggaaaataga
    13921 attgagcgcg aatgtcatcc ggcatgtatt cctgttcgac gtagtgacct gaatattcgt
    13981 gcggatattt gtaacctttt cctgcgcctt cttttctgtg cgtagtggtg ggagcatttc
    14041 gcaaatgatt tggaactgag attctttttc cttgaatgaa ctcaagggcc gcatcgattc
    14101 cacgataggc ggcattgctc ttcggacacg aggcgagaaa tgtcgcggcc tgtgcaagaa
    14161 tgattctgcc ttccggcatt cctactcttt ccagggcttg aaaggcagac acagctactt
    14221 gcagcccttg cggtgcagca tttcccacat cctccgaggc aaatatgatg attctgcgag
    14281 cgatgaaaag aggatccgca ccagcatcga tcatgatcgc cagatagagg agcgccgcgt
    14341 ctggatccga accgcgcatg gatttgatga aagcgctgat tacgtcgtaa tggttttcac
    14401 ccgcgcgatc atattgcctc gcaaagttct gtatggattt gttcagagcc tcgagagtga
    14461 tgatgtctgg ctcttctgcc gctatttcaa ccactcgaag tagcttgcga gcatcccctc
    14521 ccgcaaattg aatgagagct tcccttgcct ctccctctat agtttttcct ttgagcaacg
    14581 aatcctgcat aagggctcgc tccaggatcc gatccaggtc ttcactcgaa agcgctccca
    14641 gtttgtagac ctggcagcga gaaagcagag gagaaattac ttcgaacgac ggattctctg
    14701 tggtggctcc aatcaatacc agccagcctt cttccaccgc atgcagaaga gcatcctgtt
    14761 gtgacttgct gaatctgtgg atttcatcta caaacagtag aattcttccg gtcttcctgg
    14821 cctcttcgat cacatctctc aattctttga caccagacgc caccgcggac aggcttctga
    14881 acggcagcgt tgcttttttg gcgaggagca aagcaagagt tgtcttcccg catcctggag
    14941 gtccgtaaag gatcatcgag tgtggttttt tcccggcgag ctggtctatg agagattgct
    15001 ggccgtggaa ttcttcaaaa ctagatggcc gcagtcgctg aggaagtggc gcggccggtg
    15061 aaaaaagatg atcctgcatg ttctactaaa tatgaattgc gctatttacc gatattttta
    15121 acgtcatacg accatccagg ctaatcctgg cgagcagaat gagtcagtca gaacaaaaag
    15181 aaaccataga agagctggcg gcctctcttg agaaaatcag tcagaaaaaa ctgaagtatg
    15241 atattgtccg ccactggttc cagggccgcg agccctattt tgatctgttc gtgaatacgc
    15301 tcaatgacac tctcgtctgg tttcagttca ccttcagggg tcattccatt tgttggagcc
    15361 agcaggatcc aatcattcgc actggagtga ctacggactt tgcgctccag gacgtttctt
    15421 actattcagc tacaaagact gtgaaactcg atgaggaaat cgatccgcag tttttagctt
    15481 tctgcaaaaa actcgcaggt tgcagggcga cggaagaacc attccgatcc attctgaaag
    15541 ttctgtcctc tcctcctgat ttaacggcaa atcaatagtt cgcggtcggg cagatcttct
    15601 ttaaaatgca gttcgcgcag tcgcgcgtgc gagcagtgca attcgatcgc cccagaaaga
    15661 tcatgtagag agaccaattc atccacatcc tctctggaac gatttgcatc aggtctcgtt
    15721 cgatttgcac agcgtttttg cttttcgtaa gtccgaagat tcgagacagc cgcgctacat
    15781 gagtatccac aaccatcccg ctggggatcc catacaattc ttgaagaact acgtttgctg
    15841 ttttcctgcc gaccccgggc attgcagtga gctctgcgag agtgcgagga acaacccctc
    15901 catgctcgtg aacgagtgaa tggcaaaatc cctggatatt cttcgctttg tttctataga
    15961 aaccagtgct gtgaaccagg ttctcgactt cactcaaagg cgcagatatg tagtccccag
    16021 gatttgggaa gcgcgcgaag agcgccggcg tagtcttgtt tacctgagca tccgtacact
    16081 gtgcgctcaa gattaccgcg atgcacagtt cgtgaggttt cccataggtc agcggacatt
    16141 ttacatcccc gaactttttc tggagcaatt tgtaagcgtt ctttacaaat tcttctgcat
    16201 catcgcgttg tttcagcgcg gcgtctcctg tgatttcaaa gtccacaccc aggcaattgc
    16261 aacgatggtc atggcggatg caaagtagcc cacccattca taatgaatca gtgtgtgctc
    16321 gggacctttt gtgaggagtg caccgccaat tgctgcagat actcccgaaa acaaatgctg
    16381 cacagaggat tgaacgctca tgaacgagcc tcgatctttt ggtctagcac tggttgtgat
    16441 cattgccatg gccggaacaa atctaccgtt cacaaagatc atgaatattg tagtcacacc
    16501 aagaatggcg gccatcggaa gagggggcag actcgtgagc gctacaatgg gaatgatgga
    16561 gaacagacca atgatggaga aaactttttg ttttccatgt ctatccgcga gtcttcctgc
    16621 gtagcgagaa gagaagatcg ttgcgaggcc tccgcacaaa tagaccagag ccagatattt
    16681 tttttccagt cctacattgt aagtgagata aggagcaatg tatgggatca ctgtgaagcc
    16741 gctaaacata agaagtaccg tcaacaccat ggcgcgaagg tgagaaccat gcgtgatcat
    16801 ggagcccatc gacttgagag tctcgagcgc agatcgttta accggtgcta tgtgaccgcg
    16861 aaaagcaggg agatgtaaaa aagcgatggg ccagatgatc gcgccgatcg ctgtgatgaa
    16921 ataaaacgga acgtgccagt tgaggacttc gttcagaagc aatccagccg gaactccagc
    16981 gatggtcgcc actgaaaaag aagccatgat gatacccacg gcctgtcctc gacgagattc
    17041 tggaacggcg tcgctgacca tggaaagcac gagggctccc agaacacctc caaatgcacc
    17101 ggcgagaacg cgagcggcca tcagctgcga gtattcttgc gaaagcgcgc agagtagagt
    17161 tccaaccagg aatccaccgt aggcaatgag cactgcagtc tttctgtcga atcgatggga
    17221 ccctgtcacc gcgaatcgca ttgaccagcc tacattcatc tcttttgcct gtagagactt
    17281 tccactgggc aaatgcgcac gcatgggata ccgaccatgg accaaagctg cgacctgcac
    17341 cgggtctggc tccaagcgta gttgtagtga agtggcattg accgatcatc atcaagcgtg
    17401 tttgagaatg atgcgtgcgg actattgcgg tac
//
LOCUS       DAOQJL010000008        16580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1666_length_16580_cov_4.588381, whole genome shotgun sequence.
ACCESSION   DAOQJL010000008 DAOQJL010000000
VERSION     DAOQJL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_16580_cov_4.588381"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2218)
                     /locus_tag="PLG78_00675"
     CDS             complement(<1..2218)
                     /locus_tag="PLG78_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE21645.1"
                     /translation="MLKHDYPDASEIRRMIAEGQFHRTLQIEAIPPLVEVRSHQRGPV
                     LILERAAGHLLPTILEVGRLNTPRFLHLASSIARAVADLHGANVIHRGLSPELLLCDE
                     NKRKCYILDLSPASRLSRMNREIEKSIPMTGNPGYIAPEQTGRINRSVDHRTDLYSLG
                     IIFYQMLANRHPFVAHAPAEWAHAHIARAAPSIDRKDIPPMLLRIVTKLISKNAEDRY
                     QSALGLIEDLDRCSDELLRKGEITEFTPGEKDVSSLLHMSEKLYGRAHELDILLRAFE
                     ASRTGPLQMVLVTGSAGVGKTALIGELQKKIAESKGLFVEGKFEQYERHSPYKAWVAA
                     SQKLVERTNADAPTLAARTRERIKENVGGALGVLMESIPALRAWFNTVQEPVELPPAE
                     SRNRFTDTFVNFTTAFDQTDRPLVIFLDDVQWADAGSLDLLRSFYSIASDARILIILA
                     CRDTDVDTISSFASTLEELKKQKTFRIAKMSPLLREDVNQLIADSLLLSSDRTSALAS
                     WVHEQTEGNPLYATQLLHTLHQEGTLYFDQTAARWLWNESRLRELRDNVDLAEFLARK
                     IGGLGKDTREILSVAACLGNRFDFAILRACSGMAEEDVKAGLASALNEGLLLASEALP
                     TASAGPEGFTYQFAHDRVQQVSYQLCSDALREKIHLAAARHIQSKPDGTSTENIFALV
                     DHLNQARSLIKDRAERLEASRLNLKATRAAKANAAWAVALNTSLAAIEFLPSDSWKE"
     gene            2411..3511
                     /locus_tag="PLG78_00680"
     CDS             2411..3511
                     /locus_tag="PLG78_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE21646.1"
                     /translation="MNSSITDPVSFFLLSSGAVFALVWALGQCLERRTGSLSYLSAAT
                     LASAGLWLLSGAHFFSGLYQAFPHLLLVHLPVVACIGPCLYFYYRKLIHPSIPIRPRD
                     VAHFVPAILVAVALIPFYSLSPGEKIEIATGIRKDLLAKWFGVLNAFPKLSAIGYLSA
                     FAIRNRFLLSLRAPLNHVQLFVILFGCWIVFGTGLGLFGMILQTPSLQRWSAWTLPLI
                     IFALYLVSKRYPEFISELQVEVKRAPYGKSTLTRLDVDRIIENLQSYMKSERPFLDED
                     LKLADVADAMHITPHQLSEILNERLGRNFARFVNQYRIEEARRLLLEESDRTVLSIAL
                     AVGFNSKSAFNGAFLDFCGVTPSQFRSGQSPK"
     gene            3618..5489
                     /locus_tag="PLG78_00685"
     CDS             3618..5489
                     /locus_tag="PLG78_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE21647.1"
                     /translation="MNYKNLAEMFEASSVRFSNRPAFAWYDAHNQLHEKSYGDLYRDA
                     TYLARALVVLGVESREHVGLLSDNRYEWMVADWAVIFCGAADVPRGADITDSEITYIL
                     SHAQIKILFLENEKLFERIQNLRHSLPLLKSVIVMDPGARENPEFTRMQDLIIRGADL
                     PPESVEERVAQIRPEHLFTLIYTSGTTGAPKGVMLTHANMCSQISRMPFVPRPGDRIL
                     SILPVWHIFERVFEMIAIAGGACTYYTNVRNLREDLKRVKPSFMASAPRLWESIYSGI
                     QSNVQKASGFTRFLFRSAIFLAREVRGGIRFLRGGELDLRGRNPLISFVRALWTLFKI
                     VCLMLPFAVLDFLVLRKIRAATGGHLRGSVSGGGALPLHIDTFFNDIGIPVLEGYGMT
                     ETSPVIAVRTWKSLVPGTVGPLYPDTHIRIVDPASGARVFDSQNPKVGRGKKGEIHVR
                     GPQVMAGYFKNPEATAGVLSDGWLNTGDLGMMTFNNCLKITGRSKETIVLMGGENVEP
                     TPIESRLLQSAFIDQVMVVGQDRKTLGALIVPNRSAFDNEEYERIVASMEAQATIRKE
                     VQSLINAESGFKSFERVVVFSLVPGPFVVGDELTAKLSLKRHRIAEKHAELIESMYS"
     gene            complement(5539..6294)
                     /gene="tpiA"
                     /locus_tag="PLG78_00690"
     CDS             complement(5539..6294)
                     /gene="tpiA"
                     /locus_tag="PLG78_00690"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNE21648.1"
                     /translation="MRKKLIAGNWKMFKKIGEAQSLIKEIAQGAGDLGGVELLVCPPY
                     TALNAASEARKGTQIKVGAQDLHWEEQGAFTGKISWDMIRDTGAEYVIIGHSEQRTYF
                     HETDDTVNKKTKTALKGGLIPVICVGETLAERDGNRTMDVVSTQTKKAFEGITKEDAT
                     KCVIAYEPVWAIGTGRNATPEQAQEVHAGIRALIQSLYDASVAEGMRILYGGSMKPDN
                     AKSLLTQKDIDGGLIGGASLKADSFLAIARAGL"
     gene            6444..6656
                     /locus_tag="PLG78_00695"
     CDS             6444..6656
                     /locus_tag="PLG78_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21649.1"
                     /translation="MLHEYEDMDENYRSANFLGRKSLIATVKSDTLLPENGLRARSLL
                     SEAQFARMEVIRQRTLNKVYARFERD"
     gene            complement(6688..7884)
                     /locus_tag="PLG78_00700"
     CDS             complement(6688..7884)
                     /locus_tag="PLG78_00700"
                     /inference="COORDINATES: protein motif:HMM:NF020588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HNE21650.1"
                     /translation="MAHPIASCAEAEAFFFKYVSANDVGATGSHQAGFYMPRDSWKMF
                     FHEPGERGENRDTWLHVQWDHGTTKSRFVWYGKKTRSEYRLTNGFQFLSPDNVGDLLV
                     LAKQETNYYGFILQSDESIDEFLASFGLSPSDSGTVQFRRPSDDASLEEVIHPWARTF
                     GGEFPSGEMLSSKAREFVRPLLAPPKSEIPVTAQSKGLDLDSLLLQWLDYEFRIFRII
                     EREKYGSLLARPFESVEDLIQISHTILNRRKSRAGNSLENHLRAIFQFLGIRFSFQET
                     TEGSRRPDFTFPGKYYYDNPAFPSSALRFLGVKTTCKDRWRQILNEADRIPRKHLFTL
                     QQGVSSTQLSEMWDCGVILVVPQKHKPFFPPAFQSKIMSLREFLDELPEIPEGAEMLS
                     KEYKLF"
     gene            complement(7884..8825)
                     /gene="dcm"
                     /locus_tag="PLG78_00705"
     CDS             complement(7884..8825)
                     /gene="dcm"
                     /locus_tag="PLG78_00705"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNE21651.1"
                     /translation="MKSFRFIDLFAGIGGIRMALESVGGTCVFSSEKDKFARITYKEN
                     FGEEPHGDIYDVNVTDIPDHEVLAGGFPCQPFSIAGVSKKKSLGREHGFRDRTQGTLF
                     FKIAEILEARRPRAFLLENVKNLLSHDQGRTFQIIRETFEELGYTLFHKVLDAQGYVP
                     QHRERIFLVGFDSNVYPAPVFSFPDPPQQIVQLKSILEKSVHQKYTLSAHLWKWHQDY
                     AAKHKKNGNGFGYGLVNGDSVARTLSARYYKDGAEILVSQNPVPRRLTPRECARLMGF
                     PDSFRIAVSDTQAYKQFGNSVAVPLVTAVARSLSRAL"
     gene            complement(8822..9637)
                     /locus_tag="PLG78_00710"
     CDS             complement(8822..9637)
                     /locus_tag="PLG78_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21652.1"
                     /translation="MILSKILCSICLVLCVTVASLNAQSVPGDTNRTTFWAFEATIAR
                     GFFLHSPGAADVLGYDSSDRKPVGDYTMRYGTSFEVLRNSYAYKTAPQTDFDSSDEFR
                     LSASRKILPWLHLGLDAEHVSYRIANMHMNVRQYTNPYNQFNILTFGVISFPTLRDAA
                     VNGELLDPILLHNGWPINTITAGTHVAFHFSEGNIDPFLKVTVAVGKDLYRPLSTFEY
                     GASGGLRVFISERVFVTTEAGIRLVQFNGHAGGRTVSALLREPSFRAGAGFTR"
     gene            complement(9634..10482)
                     /locus_tag="PLG78_00715"
     CDS             complement(9634..10482)
                     /locus_tag="PLG78_00715"
                     /inference="COORDINATES: protein motif:HMM:NF027222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNE21653.1"
                     /translation="MRPVFMHPVFLLITWFILSCRPDYTKSQSDAAIQSLLFAGLSSN
                     VVTCAQPYTGQASVDTIIDQIKSTYGVQPCVNLGPLPLFTLGTVTSSDYANLQTALTT
                     LQTEFARYPIELVLRSRLRMISFPTSIGVPYGGTAGGFAPTELDFIMVRLQVSNIPFL
                     KATIHHEFFHHLDEEKNGPRYYTDPTWRSYNYTYYSYGCNRSNTSAGVISPPAAGFLN
                     TYSTCEVVEDKAEVYSNQFILPNKTQIQTLIANGDTAVAQKLDYVQRVAANFSSSINQ
                     TFWNNL"
     gene            complement(10514..11125)
                     /locus_tag="PLG78_00720"
     CDS             complement(10514..11125)
                     /locus_tag="PLG78_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21654.1"
                     /translation="MNYWKTNSVFYVNDSGGVFTGAGWTEKSAKQILWKAATLTMAEF
                     TAVVPADHRLPYSGGLQEFKKQSVPIYLSKKSRTLMLEQWKDLNLDMSSFGSWKEIPA
                     AEPDHIFDGSMEFLGGSVIVREIDAGQRSSAIAAYFPAERILFLGSALSDPPYFYTYD
                     RKALRSSITELMKLPFEIAISGHGKAIRNRAFVEDQLRQLNAD"
     gene            11267..12289
                     /gene="pyrC"
                     /locus_tag="PLG78_00725"
     CDS             11267..12289
                     /gene="pyrC"
                     /locus_tag="PLG78_00725"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNE21655.1"
                     /translation="MTIAMPDDFHVHLRDGVMLEGIVSHTAASFARAIVMPNLKPPIA
                     TTEEARLYRDRILAVAPGFTPLMTLYLTDRTVPDEVNRAKDSGFIHGIKLYPSGATTN
                     SESGVHDLEKLYPVLARMEQMDLPLLVHGEVTDTSVDVFDREKVFLDRHLKQLPRTFP
                     GLRIVFEHITTREAASFVMQSPKNVAATITPHHLLLNRNSMFRGGIRPHNYCLPVLKR
                     EEDRVALLDAATSGNQKFFAGTDSAPHERSTKENSCGCAGIYNAHAAVELYAEAFEEV
                     GKLDRLESFLSHHGADFYKLPRNKSTIRLAKKTQTVPDYYDFGGQKVVPMRAGETISW
                     SIERDS"
     gene            12279..13115
                     /locus_tag="PLG78_00730"
     CDS             12279..13115
                     /locus_tag="PLG78_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HNE21656.1"
                     /translation="MIRETIEMTTRVSDLDTQRHVTSRTYESFCLEGRHRLLEKQGYS
                     VGRLLDEGIHLVPTHSYVRFMYQQYAGAVLVIHTTAASDSKKGIIWDQKVEQLDGKPV
                     CHIQLRTEFFQGEDRLTLMDAGDAEFEILYEDLAEFKGGAQQIESSYTPLYCERDIFG
                     SYSPSLLWKVFEEGRWLFCARTGLTYEKFVEMDTTSFYMGGVFNFFGRMIAGKPLRIR
                     TWIDSLEKIRFYFRQDVFDGDRLLMSLRDEQLIVSLKNARPRKAPTEFISFIESYIEK
                     SK"
     gene            13112..14179
                     /locus_tag="PLG78_00735"
     CDS             13112..14179
                     /locus_tag="PLG78_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE21657.1"
                     /translation="MNSSNWLLYGATGFTGELIVEEAVRQGMKPVIAGRSEARIREMA
                     EKFGLEWKVFSLVDPQSIRAGIRGFACVLHAAGPFIYTAEPMMHACIDEGAHYLDITG
                     EIPVFQIATRLGSRAEDTGIALIPGVGFDVVPTDCLARYVADASPGSENLEIAFAAIS
                     NPSQGTAKSAIEMMPRGFLTRRNGDLQRISPGSGGKKVQFSDKERFVLPVSWGDLETA
                     FYSTGIANITTYTSLSRGASMFLRVFGPVMTLLFQFSPVRRSAKKFVDLFVRGPGARE
                     QKSGRSYVYVKVSSAAGKSREAWLETIEGYVFTAKSALLCVKEVIEKSPKGFKTPSVA
                     FGYDLVLRIPETRILDSIGNQ"
     gene            complement(14173..14946)
                     /locus_tag="PLG78_00740"
     CDS             complement(14173..14946)
                     /locus_tag="PLG78_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21658.1"
                     /translation="MPILANKTPDELEPIILNALDKGQILSVQTYYLSDYGEKVLNLV
                     TSAILKKYGREDLIDVVYTSAKELVINATKSNLKRVLFARIGLDLFNPENYEQGMKHF
                     KEELKEENIRKYKSLFREFKFTVTITFYYSPDVLNIKIKNNTQLIPQEEERIRQKFAQ
                     AVSYANLFDFYLEHGDDTEGAGMGLTLVGILLNQSGIDKHQFTLYSSPHYNETTARLE
                     IPLSQEYTSRRERFEKERESSGLSREEFRKHFDSKKEFH"
     gene            complement(15038..16015)
                     /locus_tag="PLG78_00745"
     CDS             complement(15038..16015)
                     /locus_tag="PLG78_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE21659.1"
                     /translation="MRILLLLVSFTFAFCGRKENALLNVSYDPTREFFHEYNELFQTH
                     WKKVSGEDIQVTQSHGGSGKQARAVIDGLPADVVTLGCSYDIDRIAARGLLPADWQQK
                     LPNNSSPFYSTIVFVVRKGNPRNIHDWPDLVAPGVTVILPNPKTGSSARWAYLAAYGQ
                     AKKTGGKAAADEYIRKLIAAVPIMDTGARAALTTFAERGMGDVFLSWENDAYLALHEH
                     PDLQIIVPPVSIRAEPAVAVVEKNAGPRLKAAQEYLTYLYSEEAQDLAGRHHYRPRSA
                     AAASRYASTFPNLILLDLTEVAGSWAQADKEHFADGASFDQAYQKRSRP"
     gene            complement(16060..>16580)
                     /locus_tag="PLG78_00750"
     CDS             complement(16060..>16580)
                     /locus_tag="PLG78_00750"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21660.1"
                     /translation="IVATGAASTPREILLHLNDVLIKQIRPGELTSFATAVLLLIDPV
                     EMSVQMARCGHELPIFWNPGNREMREIKTSGFMLGVSPSSMMSGVLNETSVQLARGDA
                     IYIYSDGVTEAANVDGEFYGLSRLKSAIRAFGSGPAQELLDVLLSDIRAFSGIAPQHD
                     DITLVIVRLLGP"
BASE COUNT         3939 a         3985 c         4263 g         4393 t
ORIGIN      
        1 cctccttcca ggaatcagaa ggcaaaaatt caatcgccgc aagactggtg ttgagtgcga
       61 ctgcccatgc tgcatttgct tttgctgcgc gagttgcttt gaggttgagt ctagatgctt
      121 cgagtctttc tgcgcgatct ttgatgaggg atcgtgcttg attgagatga tctaccagag
      181 caaaaatatt ttccgtggaa gtgccgtccg gtttggattg aatgtgcctt gctgccgcca
      241 ggtgaatttt ttcgcgtaaa gcgtccgagc atagctggta ggaaacctgt tgaacgcgat
      301 catgagcgaa ttgatacgtg aagccctccg gaccggcgga ggcagtaggc agagcttcgg
      361 atgcaagcaa caagccttcg ttgagtgccg aggcaaggcc cgctttcaca tcttcttctg
      421 ccatcccgga acacgcgcgc aaaatagcaa aatcgaaacg gtttcccagg caggccgcca
      481 cagatagtat ctctctcgta tctttgccaa gccctccgat ctttcgcgcg agaaattctg
      541 caagatccac attgtctcgc agttccctga gcctggattc attccatagc cagcgcgccg
      601 cggtttgatc aaaatagaga gttccttcct ggtgcagagt gtgcagaagc tgcgtcgcat
      661 agagaggatt gccttctgtt tgttcgtgga cccagcttgc cagagcagac gtgcgatcgc
      721 tcgagagtag aaggctatca gcgatcagct ggtttacgtc ttcgcgcaag agaggggaca
      781 tctttgcgat gcgaaaggtt ttctgtttct tgagttcttc cagagtggat gcgaaactgg
      841 aaatcgtatc cacatctgta tctctgcatg cgagtatgat gagaatgcgc gcatcggatg
      901 cgatcgagta aaaggagcga agtagatcca atgatccggc atccgcccat tgcacgtcgt
      961 cgaggaagat caccagaggc ctgtccgttt gatcgaacgc agtcgtgaag ttcacaaacg
     1021 tatctgtaaa acggtttctg gattctgcag gcggaagctc cactggttcc tggactgtgt
     1081 tgaaccacgc tctgagcgct ggaatgctct ccatcagaac tcccagagcg ccgcccacgt
     1141 tttctttgat cctctctctg gtccttgcag caagcgttgg cgcatctgcg ttggttcttt
     1201 ccaccagttt ttgagaggcg gccacccacg ccttgtaagg cgaatgtctt tcgtattgtt
     1261 cgaacttgcc ctctacaaat agaccttttg actccgcaat cttcttctgc aattctccaa
     1321 tgagtgccgt cttcccaacg cccgcgctac ctgtgaccag aaccatttgg agtgggccgg
     1381 ttcgcgaagc ctcgaaagcg cgaagaagta tgtccaattc atgtgcgcga ccgtacagtt
     1441 tttcagacat gtgaagcaaa gaggatacgt ctttttctcc cggagtaaat tctgtaattt
     1501 ctccttttcg cagaagttca tcggagcagc ggtccaggtc ttcgattaat cccagtgcgg
     1561 actgatagcg atcctccgca ttttttgaaa tcagtttcgt gacgattcgc agaagcatgg
     1621 gaggaatgtc tttgcgatcg atagatggcg cggcgcgtgc aatgtgagcg tgcgcccatt
     1681 ccgcaggagc gtgtgctaca aaaggatgcc tgttggccag catttgataa aagatgatgc
     1741 caagtgaata gagatctgtg cgatgatcca cactgcgatt gattctccct gtttgttcgg
     1801 gagcgatgta tccaggattt cctgtcatcg gaatgctctt ttcaatttcg cgattcattc
     1861 gcgagagtcg cgatgccgga ctcagatcca ggatgtaaca cttgcgttta ttctcgtcgc
     1921 ataacagaag ttcggggctg agtccgcgat gaataacatt ggctccatgc aaatctgcca
     1981 cggcgcgcgc gatcgaagaa gccagatgca aaaatcgcgg cgtattcaat cggcccactt
     2041 cgagaatggt tggcagtaga tgccccgcag ccctctccaa tatgagaacc ggtccgcgct
     2101 ggtgagatcg gacttctaca agagggggaa tagcttctat ctggagtgtg cgatgaaact
     2161 ggccttctgc aatcatacgc cggatttcgg atgcatccgg atagtcatgc ttcagcatct
     2221 tgatgagtac tggctttctt tgatctttga ggaatccttc ataaatgatg gctttctcta
     2281 attctgtaag aagttcgccg gtgatgatgc caggaaaatt cagaaggacc tccccaaaag
     2341 ttcagcctgt ttcaaattca caaatgcaca gcggttgacg aaaagtgtcc accatagttg
     2401 gcttgtccca ttgaattcgt ctatcacgga ccctgtcagc ttttttcttc tgtcaagtgg
     2461 tgcggttttc gcgctcgtct gggctctcgg acagtgtctg gagcgtcgca ctggctcgct
     2521 gtcttatctt tctgccgcta ctctggcttc tgccgggtta tggcttttgt caggagctca
     2581 tttcttttct ggtttgtacc aggcttttcc tcatctcctg ctcgttcatt tgcccgttgt
     2641 agcctgcata ggtccttgtc tgtattttta ctatcgcaag ttgatccacc cttctattcc
     2701 gattcgtccc agggacgtgg cccatttcgt tcccgcaatt ctggtagcgg tggcgctcat
     2761 tcctttttac tcgttgagtc cgggagaaaa gattgaaatc gcgaccggaa tcaggaagga
     2821 tctgcttgcg aagtggttcg gggttttgaa cgcgtttcca aagctttcgg ccatcggcta
     2881 cctttctgca tttgcgattc gcaatcgatt tctcctcagt cttcgcgcgc cattgaatca
     2941 tgtgcagctg tttgtcatcc tgtttggatg ctggattgtg tttggaacag gtctcggttt
     3001 gtttggaatg attttgcaga cgccttccct gcagcgttgg agtgcctgga ctttgcctct
     3061 gatcattttt gctctttatc ttgtgtccaa aagatatcct gagtttatca gcgaactgca
     3121 agtcgaagtg aagcgcgctc cttatggaaa gtcaacgctg accaggctcg acgtggatcg
     3181 cattatcgag aatctacaaa gctacatgaa gagtgaacgg ccttttctgg atgaagatct
     3241 caaactggca gacgtggcgg acgcgatgca cattacacct caccagcttt ccgaaatttt
     3301 gaacgaacgg cttggccgta attttgcgcg tttcgtcaat cagtacagga ttgaagaggc
     3361 aagaagactt ttgttggagg aatcagacag aactgttctt tcgattgcac ttgctgttgg
     3421 attcaattcc aaatctgcat tcaacggagc tttccttgat ttttgcggag ttactccctc
     3481 gcagtttaga tctggccaat caccgaagta gcgaatgata aaaccaaacc tctacgcgta
     3541 gttcggatag atcaatcagg acgacttcgg ttcttccgag tgttaaaatt ttctccagga
     3601 tgattcctgg aggggatatg aattacaaga atcttgcgga gatgtttgag gcttcgtccg
     3661 ttcgcttttc gaatcgaccg gcgtttgcct ggtatgacgc acacaatcaa ctgcacgaaa
     3721 aaagctatgg cgacctttat agagatgcaa cgtacctcgc tcgtgcgctc gttgtgcttg
     3781 gtgtcgagtc tcgtgaacat gtcggtctat taagtgacaa tcgatatgaa tggatggtcg
     3841 cagactgggc agtcatcttt tgcggtgcag cggacgttcc acgcggcgca gacatcacgg
     3901 actcggaaat cacttacatt ctgtcccatg cacagatcaa gattctattc ctggaaaatg
     3961 agaagctctt tgaaagaatt cagaatctga ggcattcgct tccattgctt aagtccgtga
     4021 tcgtaatgga tccgggcgcg cgcgaaaacc ctgagttcac gcgcatgcag gatttgatta
     4081 tacgtggtgc cgatttgcct ccagaatctg tggaagagcg agtggcacaa attcggccgg
     4141 aacatttgtt cactctcatt tatacgtcgg ggacaaccgg tgcacccaaa ggagtgatgc
     4201 tcacgcatgc aaatatgtgt tcgcagattt cgagaatgcc ctttgttccc agaccagggg
     4261 atcgcattct ctccatcttg cctgtgtggc atatatttga gcgcgttttt gaaatgatcg
     4321 ccattgcggg tggagcctgc acgtactaca cgaacgtgcg aaatttgcgc gaagacttga
     4381 agcgagtgaa gccatccttt atggcgtcag cgcctcgtct gtgggagagt atttattccg
     4441 gcattcagtc gaatgtgcag aaagcgtccg ggttcactcg cttcctattt cgctctgcga
     4501 tctttcttgc acgtgaagtc cgcggcggca tccgattctt gcgaggcggc gagttggact
     4561 tacgtggacg aaaccctttg atctcctttg tgcgggcgct gtggaccttg tttaagatcg
     4621 tatgtctcat gctgccgttt gctgttctcg atttcctggt tttgcgaaag attcgcgcgg
     4681 ctacaggagg tcacctgcgt ggttcagttt ctggcggagg agccttgccc ttacacatag
     4741 atacattctt caacgatatt gggattccgg ttttggaagg ttatggcatg acagagactt
     4801 cgcctgtgat tgcagttcgc acgtggaaat ctctggttcc agggaccgtt gggcctttgt
     4861 acccagacac acacattcgc attgtagatc ccgcgtccgg ggctcgcgtt ttcgattcac
     4921 agaatcctaa agtggggcgc ggcaagaaag gcgaaatcca tgtcagaggt ccgcaggtga
     4981 tggccggata tttcaagaat cccgaggcaa cggccggagt gctttccgat ggctggctca
     5041 acacgggaga tctgggcatg atgactttca acaattgcct gaagatcaca ggacgttcca
     5101 aagagacgat tgtgcttatg ggcggtgaga atgtggaacc cactcccatc gaaagcaggc
     5161 ttcttcaatc tgccttcatt gatcaggtta tggttgtagg tcaggatcga aaaacactgg
     5221 gagcgctcat cgtgcccaat cgatctgcct ttgacaacga ggaatacgag agaatcgtcg
     5281 caagcatgga agcccaggcc accattcgaa aagaagttca gtctttgatc aatgctgagt
     5341 ccggcttcaa gtcatttgag agagtggtgg tgttttcgct tgttccaggt ccattcgtgg
     5401 ttggagacga actaacagcg aagctttctc tgaaacgtca tcgcattgca gagaagcatg
     5461 cggaactcat tgagtctatg tattcatgag aaatcggagc gtcctcctga cggggaagcc
     5521 cgtcaggcga cattcaaatt aaagacccgc gcgtgcaatg gccaggaagc tgtccgcttt
     5581 cagggaagca cctccaatga gaccaccatc aatgtctttt tgagtcaaaa gtgacttagc
     5641 attgtccggc ttcatgcttc cgccgtagag gatacgcatt ccctctgcaa cggatgcgtc
     5701 atagagagac tgaatgagcg cacgaattcc tgcatgcact tcctgagcct gttctggagt
     5761 tgcatttcta cctgtgccaa tggcccagac cggttcataa gcgatcacgc acttagttgc
     5821 atcttcttta gtaattcctt cgaatgcttt tttcgtctgt gtggagacaa catccatggt
     5881 tctgtttccg tctctttccg cgagagtttc ccctacacag atcaccggaa tgagtccgcc
     5941 tttgagagct gtcttggtct tcttgtttac tgtatcatct gtttcgtgaa aatacgtgcg
     6001 ctgctctgag tggccgatga ttacatattc tgcgccggtg tcgcgaatca tatcccagga
     6061 aatcttcccg gtaaatgcgc cctgttcttc ccagtgcagg tcctgcgcgc cgaccttgat
     6121 ttgagtgcct tttctggctt cgcttgctgc gttgagtgca gtgtatggcg ggcagaccag
     6181 gagttccacg cctccaagat ctccggctcc ttgcgcaatt tccttgatca gactttgcgc
     6241 ctccccaatt ttcttgaaca tcttccagtt tcccgcgatc agtttctttc tcatgccccg
     6301 agcgtgggaa aatagacgcg ggagtaaagg aggttttttt tcagcagagt gcaaaaaaga
     6361 ctgaatggtc cagcaaaaca gtcgatagta ctatcaggcg gtatatacac ggaagagcag
     6421 ccgccttctg cacggaggca gaaatgttac acgaatacga agacatggat gaaaactatc
     6481 gttccgcaaa tttcctgggt cgcaaaagcc tcatcgcaac ggtgaagagc gatacgttgc
     6541 tacccgaaaa cggattgcgc gcaagatctc ttttgagcga agcacagttc gcaagaatgg
     6601 aagtgattcg ccagcgcact ctcaacaaag tctacgctcg ttttgaaagg gactgaacag
     6661 gcagccaccg tccgccaggg tccagcgtca aaaaagctta tattctttgc tcagcatttc
     6721 ggcgccttcc ggaatttccg gaagttcgtc gaggaattct cgcaaagaca taatcttgct
     6781 ttgaaatgcg ggcgggaaaa acggcttatg cttttgtggg accaccagga tcactccgca
     6841 atcccacatc tcagagagtt gcgtagacga gactccctgt tgtagagtga acaaatgctt
     6901 tcgcggaata cgatccgctt cgtttagaat ctggcgccat ctatccttgc atgtggtctt
     6961 taccccaagg aaacgcagag cagaagatgg aaaggctgga ttgtcgtagt aatactttcc
     7021 tggaaaagta aaatccggtc tcctcgatcc ctctgttgtt tcctgaaaag aaaaacgaat
     7081 tccgagaaat tgaaaaatcg cgcgaagatg gttctcaaga gaattgcccg ctctggattt
     7141 tcttctattt aagattgtgt gagatatctg gatcaaatct tccacggatt caaaaggacg
     7201 agccagcagg ctcccgtatt tttctctttc tatgattcga aaaattcgaa attcgtagtc
     7261 cagccactgc aataagagtg agtccaggtc caggcctttg ctttgcgcag ttacggggat
     7321 ttctgattta ggcggtgcca ggagcggtct gacgaattcg cgcgctttgg agctgagcat
     7381 ttctcctgat ggaaactctc ccccgaacgt tcgggcccac ggatgaatga cttcttctaa
     7441 agaggcatcg tcggatggtc tgcgaaattg aacggttcct gaatcagaag gggaaaggcc
     7501 aaaggatgcc agaaactcat cgatggactc atctgattgc aaaataaacc catagtaatt
     7561 tgtctcctgt ttcgccagaa ccagaagatc tcccacgtta tccggcgaaa gaaattgaaa
     7621 tccatttgta agtctgtact cggatcttgt ttttttgccg taccatacga atctgctttt
     7681 tgtggtccca tggtcccact gtacgtgaag ccaggtatct ctgttttctc ccctttctcc
     7741 tggctcatga aaaaacattt tccatgaatc gcgcggcatg tagaatcctg cctggtggga
     7801 accggtagct ccaacgtcgt tggcactgac gtacttgaag aagaatgctt ccgcttccgc
     7861 acacgatgca attgggtgag ccactagaga gccctcgaaa gactgcgcgc aacagcagtg
     7921 accaggggaa cggcaacgga gtttccgaat tgcttgtaag cctgcgtatc tgaaaccgca
     7981 atccgaaacg aatccggaaa gcccatcaat cgcgcgcatt ctcttggcgt gagtcgacgt
     8041 ggaacaggat tttgagatac cagaatttcc gctccgtctt tgtagtatcg cgcgctgaga
     8101 gttctggcta ctgagtctcc gtttacgagt ccatacccaa aaccatttcc gtttttctta
     8161 tgtttggcgg cgtaatcctg atgccatttc cacagatgcg cagataaagt gtatttttga
     8221 tgtaccgatt tttctagaat gcttttcagt tgcacgatct gctgcggtgg atctggaaat
     8281 gaaaagacag gagcaggata tacattggaa tcaaagccta cgagaaagat tctttctcta
     8341 tgctgtggaa cataaccctg cgcatcgagt actttatgaa acagagtata gccgagttcc
     8401 tcaaaggttt cgcgaatgat ctggaatgtg cgtccttgat cgtgcgaaag caaattcttt
     8461 acattctcca gtagaaatgc acgcggacgc ctggcttcca gaatctcggc gatcttaaag
     8521 aagagcgtgc cctgggttct gtctcgaaaa ccatgctctc tacccagact cttctttttg
     8581 gatactccgg cgatggagaa tggctggcat ggaaaacctc cagccagaac ttcatggtcc
     8641 ggaatgtctg tcacgttgac atcgtaaata tcgccgtgcg gttcctcccc gaagttctct
     8701 ttgtacgtga ttcgagcgaa tttgtccttt tcagaggaaa agacgcatgt tcctcccaca
     8761 ctctccaggg ccatgcgaat gccgccaatt ccggcgaaca aatcaatgaa tcggaaagac
     8821 ttcaacgagt gaatccggcg cccgcacgaa aagatggttc tctcaaaagg gccgaaactg
     8881 tacgcccgcc agcgtgtcca ttgaattgga ccagtcgaat gcctgcctcc gttgtaacga
     8941 atacgcgctc gctgatgaag acgcgcaatc cgccggatgc accgtattca aatgtgctga
     9001 gtggtcgata tagatctttg cccactgcaa ccgttacttt tagaaatgga tctatgtttc
     9061 cttctgagaa gtgaaatgca acatgagttc cggctgtgat tgtgttgata ggccatccgt
     9121 tgtgaagtag aatcgggtcg agcaactcac cgtttacagc agcatcgcga agagtgggga
     9181 acgaaatgac tccgaaggtt agtatgttaa actgattgta tggatttgta tactgtctaa
     9241 cattcatatg catgtttgca attcgatagc ttacatgctc tgcatccaga cccaggtgaa
     9301 gccacgggag aatctttctg gaagcagaaa gtctaaattc atcggaggaa tcgaaatctg
     9361 tctgaggagc tgttttgtag gcgtagctgt ttcgcaatac ttcaaaagaa gtaccgtatc
     9421 tcatggtgta atcgcccact ggctttcggt cagatgaatc gtagccaagc acgtcagctg
     9481 cccctggcga atgaaggaag aagcccctgg cgatggttgc ttcgaatgcc cagaatgttg
     9541 ttcggttcgt gtcgcccggc accgattgcg cgttcaggga cgctacagtc acacaaagta
     9601 ccagacaaat tgaacaaagg atcttactga ggatcatagg ttattccaga aagtttggtt
     9661 gatgctggag ctgaaattcg ccgcgaccct ctggacgtag tctagcttct gtgcgaccgc
     9721 tgtatctcca ttcgcgatga gtgtttggat ttgcgtcttg tttggcagga tgaattgatt
     9781 ggaatagact tctgccttgt cttccaccac ttcacatgtg gagtacgtat tgagaaaacc
     9841 ggcggctgga ggagagatca ctcctgcaga tgtattggat cgattgcagc cgtaggaata
     9901 gtaagtataa ttataggatc tccaggttgg atccgtatag tagcgagggc catttttctc
     9961 ttcatccagg tggtgaaaga attcatgatg gatggttgct ttgaggaagg ggatgttcga
    10021 cacctgaagc cgaaccatga tgaagtccag ttcggtgggc gcaaatccgc ctgcagttcc
    10081 tccgtacgga actccaatag aagttggaaa agaaatcatt ctgaggcgag agcgaagcac
    10141 gagttcaatg ggatatcgtg cgaattcagt ctgcaatgtg gtgagtgctg tttgtagatt
    10201 tgcgtagtcg cttgaagtaa cggtacccag cgtgaaaaga ggaagcgggc ccaggttgac
    10261 gcatggctgt actccgtacg tagatttgat ctggtcgatg atcgtatcta cggatgcctg
    10321 gcctgtatat ggttgtgcgc atgtgacaac attggagctg agacctgcga agagcaaaga
    10381 ctgaatggct gcgtctgatt gactcttcgt gtaatccggg cggcagctca ggatgaacca
    10441 ggtgatcaat aggaagactg gatgcatgaa gacggggcgc atagccaacg atcgcatgcg
    10501 ctgcgataca cggtcaatcc gcattcaatt gccgcagctg atcttccacg aaagctcgat
    10561 tgcggatggc cttgccgtgt ccgctgatgg cgatttcaaa tggaagcttc attagttctg
    10621 tgatcgagga ccgaagcgcc ttacgatcgt acgtgtaaaa gtaaggcgga tcggaaagag
    10681 cgctgcctaa aaagaggatg cgctctgccg gaaagtaggc tgcaatcgca gaagatctct
    10741 gtcctgcatc tatttctcgc acaatcacag agccgcccag aaactccatc gaaccatcaa
    10801 agatgtgatc cggctccgcg gctgggattt ctttccagga cccaaagctg ctcatatcca
    10861 ggttcaagtc cttccattgc tccagcatca acgtcctgga ttttttggaa agatagatgg
    10921 ggacagactg cttcttgaat tcttgcagcc caccgctgta aggcaatctg tggtctgctg
    10981 gaacaacggc ggtgaattca gccatagtca gtgtggcggc cttccataaa atctgcttcg
    11041 ccgatttttc agtccatccg gcgcccgtga agactccacc ggaatcattc acatagaaga
    11101 ctgaatttgt tttccagtaa ttcaagtctt ccaccaggta aacaggcccg cggatccgag
    11161 taattcggat cggacgatcg gacgcgtcga ctggctcctt gattgggccg catgacgcaa
    11221 ttatcattgc actcagccag acgacgaaaa gcctgagcat gtgcagatga ccattgcaat
    11281 gccagacgac tttcatgtac accttcgcga cggggtgatg cttgagggaa ttgtttccca
    11341 tactgcggca agttttgcgc gggcgattgt aatgccaaat ctgaaaccac ccattgcgac
    11401 gaccgaagag gcgcgcctct acagagatcg catcctcgct gtggcccccg ggttcacacc
    11461 tctgatgact ctctatctga cggaccgtac tgtgccggat gaagtcaatc gcgcgaaaga
    11521 ttcgggtttt atccacggaa tcaaactcta cccgtccgga gccaccacca attcagaatc
    11581 cggagtccat gatctagaga aactctatcc agtgcttgcc cggatggaac agatggatct
    11641 gcctttgctg gttcacggcg aagtgacaga tacatccgtg gatgtgtttg atcgagagaa
    11701 agtctttctg gatcggcact tgaagcagct tccacgaaca tttccaggcc ttcgcattgt
    11761 attcgaacac atcacgactc gtgaagccgc atcttttgtg atgcagtctc cgaaaaatgt
    11821 ggccgctacc attacgccac accatctgtt attgaatcgc aattctatgt ttcgcggagg
    11881 aattcgtccg cataactact gtttgccggt actgaaaagg gaagaggatc gagttgcgct
    11941 tctggatgcg gccacgtcgg gcaatcagaa attcttcgcc ggaacagaca gtgctcccca
    12001 cgagcgatcc acaaaagaaa attcgtgtgg gtgtgccgga atctacaacg ctcacgcggc
    12061 agttgaactt tacgcagagg catttgagga agtcgggaag ctggatcgtc tggaatcgtt
    12121 tctgtcccat catggtgctg atttctataa actgccccgc aacaaaagca ccatccgact
    12181 ggcgaaaaaa acgcagacgg tgccggatta ctatgacttt ggcggccaga aggtagtgcc
    12241 tatgcgcgcc ggagagacta tttcttggag catagagcgt gattcgtgaa accatagaaa
    12301 tgacaacgcg tgtttcagac ctggacacgc agcgtcacgt gaccagtcgc acgtatgaaa
    12361 gtttttgcct ggaaggaagg catagactgc tcgaaaagca gggctattca gtcggacgac
    12421 ttctggacga aggaattcat cttgtcccca ctcattcata tgttcgattc atgtaccagc
    12481 agtatgctgg cgctgttctt gtgattcata ccactgcagc ctccgatagc aagaaaggaa
    12541 ttatctggga tcagaaggtg gagcagttag acggcaaacc tgtctgtcat attcaactgc
    12601 gtacggaatt tttccaggga gaagatcgtc taacgttgat ggatgcaggg gatgcagaat
    12661 ttgaaattct gtatgaggat cttgctgaat tcaagggagg agcgcagcag attgaaagtt
    12721 cgtacactcc tctgtattgt gaaagagata tttttggttc ttacagtccc tcgcttctct
    12781 ggaaagtctt tgaagaaggt cgatggttat tttgcgcacg cactggcttg acctatgaga
    12841 aatttgtaga aatggacacc acatccttct acatgggggg agtgtttaac ttcttcggaa
    12901 gaatgatcgc tggcaaacct cttcgcattc gtacctggat tgattccctg gagaaaatcc
    12961 gattctattt caggcaggac gtatttgatg gagacaggct tctcatgtct ttacgcgacg
    13021 aacagttgat agtttctctc aagaatgcca ggcctcgcaa agctcccaca gaatttatat
    13081 ccttcattga atcatacatt gagaaaagta agtgaattcg agtaactggt tgttatacgg
    13141 agctaccggt tttaccggtg agctcatcgt ggaagaagcg gtccggcaag gaatgaagcc
    13201 tgtgatcgcc ggccgatcgg aagccagaat ccgcgaaatg gcggaaaaat tcggactgga
    13261 atggaaggtc ttttccctgg ttgatccgca gtccattcgc gccggtattc gtggatttgc
    13321 atgcgtactt catgcggccg gtccttttat ttataccgcg gagcccatga tgcatgcctg
    13381 catcgacgag ggcgctcatt acctggacat tacaggagaa attcctgtgt ttcaaatcgc
    13441 cacgaggctt ggttcgcgcg cagaagatac aggcattgct ctcattcctg gagtgggatt
    13501 tgacgtagtg cccacagatt gcctcgccag atatgtggcg gacgcaagtc ctggatcaga
    13561 aaacctcgag atcgcttttg ctgctatttc caatccaagt cagggcacgg ctaagtctgc
    13621 catagagatg atgcctcgcg gctttttgac cagaagaaat ggcgatctgc agagaatctc
    13681 tccgggaagc ggcggaaaga aagttcaatt ttcagacaaa gaaagattcg tgctccccgt
    13741 ttcctggggc gatctggaaa ctgcttttta ctctacaggc attgcaaaca ttacaactta
    13801 cacttcgctt tcgcgcggtg cctcgatgtt tctcagagtg tttggtccgg tgatgacgtt
    13861 gttgttccag ttttcgcctg tgaggcgatc ggccaaaaaa tttgtggatc tattcgtgcg
    13921 aggacccggc gcaagagaac agaaaagtgg aagatcgtat gtatatgtca aagtttcttc
    13981 tgctgcgggt aaaagcaggg aggcgtggtt ggaaactata gaaggctatg tgtttacagc
    14041 gaagagcgca ctcctttgcg tgaaagaagt gattgaaaag agcccgaaag gattcaagac
    14101 accgtccgtc gctttcggtt atgatctcgt gcttcgcatt ccagaaacca gaatactgga
    14161 ttcgattggg aatcagtgaa attctttttt gctatcgaag tgcttacgaa actcttcgcg
    14221 gctcagtccg cttgattctc tttctttctc aaatctttcg cgtctgcttg tgtactcctg
    14281 ggacaaagga atctcaagtc tagcagtggt ttcgttgtag tgtgggcttg agtatagagt
    14341 gaattgatgt ttatcgattc cactttgatt aagtagaatg cctaccagag tcagacccat
    14401 gcctgcgcct tctgtatcat ctccatgttc cagatagaaa tcaaagagat tcgcgtaaga
    14461 aaccgcctgt gcaaactttt ggcggatgcg ttcttcttcc tgtggtatca attgagtgtt
    14521 gttcttgatt ttgatgttta gaacgtcagg cgagtaataa aacgtgatgg tcactgtgaa
    14581 cttgaattcg cggaaaaggg atttgtattt tcgaatgttc tcttctttga gttcttcttt
    14641 aaaatgtttc atcccctgtt catagttttc tggattgaat aggtccaggc ctatgcgggc
    14701 aaaaagaact ctcttcaaat tagacttggt cgcgttgatg acgagttctt ttgcggacgt
    14761 atacacaacg tcgatgaggt cttcgcggcc gtacttcttc agaatagcgg aggtgaccag
    14821 attgagtact ttttcgccgt aatcgctgag gtaataggtc tgaacggaaa ggatctggcc
    14881 tttgtccaga gcattgagaa tgatgggctc aagctcatcg ggtgttttat tggccaggat
    14941 gggcatgtta gtgaaatgag tgcctcgggc agaacctgac agcctctcct attgtcgtcc
    15001 agtcaatacc gggcatgtag caaaaacaac tacgatttca cgggcgtgat cgcttctggt
    15061 aggcctgatc aaagctggcc ccatccgcaa aatgctcttt gtccgcctgc gcccagctgc
    15121 cggcaacttc ggttaggtcc agtagaatca ggttcgggaa cgtggaggca taacgagatg
    15181 cagctgcagc tgaccgcggg cgatagtgat gcctgccagc cagatcctga gcctcttcgg
    15241 aatatagata cgtcagatac tcctgcgctg ccttcagcct gggccctgcg ttcttctcca
    15301 caacggctac agctggttcc gcgcgaatgc ttacaggtgg aacgatgatt tgaaggtctg
    15361 gatgttcgtg taacgccaga tatgcgtcat tttcccacga caggaacacg tctcccatac
    15421 ctctctccgc aaatgtggtg agagcggctc ttgcgcctgt gtccatgatg ggaacagctg
    15481 cgatcagttt gcggatgtac tcatctgccg cagccttccc tcccgttttc tttgcctgcc
    15541 cgtaggccgc gagataagcc catcgcgcgc tacttcctgt ttttggattg gggagaataa
    15601 cggttacacc aggggcgacc aggtctggcc agtcatgaat gttccgcgga tttcctttcc
    15661 tcactacaaa cacaatcgtg gaataaaaag gagaagagtt gttcgggagt ttttgttgcc
    15721 agtccgctgg aaggagtcct cgcgcggcga ttctgtctat gtcgtaggaa catcctaacg
    15781 taactacatc tgcagggagt ccatcaatca cggcgcgagc ctgttttcct gaaccgccgt
    15841 gcgactgagt cacctgaatg tcttcaccac tcactttctt ccagtgagtt tgaaacagct
    15901 cgttgtattc atggaagaac tctcgcgtgg gatcgtaaga tacattgaga agggcgttct
    15961 cttttctccc acaaaatgca aaagtaaatg aaactaacag aagcaggatt ctcacaggag
    16021 catgccgtcc tgtttgcgcg aatccgtcca ggtttttttc tacgggccga gcaagcgcac
    16081 gatcaccagc gtaatgtcgt catgctgtgg agctattcca gaaaatgcac gaatgtcaga
    16141 tagaagaacg tcgagcaact cctgtgcagg tccgctgccg aatgcgcgga tagccgattt
    16201 caatctggaa agaccgtaaa attctccgtc cacgttggct gcttccgtca ctccgtccga
    16261 atagatgtag attgcatctc cgcgagccaa ttgcactgaa gtttcattga gcactccgga
    16321 catcatcgaa gaaggagaca ctccgagcat gaagcccgat gtttttattt ctctcatttc
    16381 ccgattgccc ggattccaga agatcgggag ttcatgtccg cacctggcca tttgaacgct
    16441 catttccaca ggatcgatca gaagcaagac ggctgtcgcg aatgaagtga gttcccccgg
    16501 acgaatttgt ttgatcagga cgtcattcaa gtggagcagg atttcgcgtg gagtggaggc
    16561 cgctcccgta gcaacaatgg
//
LOCUS       DAOQJL010000009        16397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1691_length_16397_cov_4.707625, whole genome shotgun sequence.
ACCESSION   DAOQJL010000009 DAOQJL010000000
VERSION     DAOQJL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16397
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_16397_cov_4.707625"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2697
                     /locus_tag="PLG78_00755"
     CDS             <1..2697
                     /locus_tag="PLG78_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNE21661.1"
                     /translation="GRPFTDGSVGFHFAFEGYNIGTATQVNLSPAQCAWTPATQAPAS
                     ISGQVDNAANVAAASGLTVKLISSGGSVVAQTTTDGSGAFTLSASSVAGGIYRIEISG
                     SAYNTQNASLLFVPLATGASTSVNAGSISVIPKASNIAGTIRDAATSAGIAGAVISFR
                     PAASQPVSKLTYLLTDPVLGAAAARWIAEKNGTGSYSTYLTKSYNGPDAGGNLYMTAV
                     AGKWAYYVSAAGYQSSTAGEITLNGADETANISLTSDPKRSRISGQAVVVDTLIDGSK
                     QSYGATVAGYTNQGYALSGLIVVMLNNRNNAGDAVAHVTTTSAAGTFTFTAQHVVLPA
                     GLTDDSARVAYAASQFAGGNVTTANISGTDSVSDSITTDGTNYWFKSNSYSIYIIDPR
                     GHMSGSLTTATNGSVASNTPTAATTLSVGAIAAHLPRGKISGVVTDAFSTEAISGALV
                     QLGRDTNPDPSLISFSPARKDSNPIANVSRLSNSADVQVADVTTPSNGSYSFDNIDPG
                     DYVLQISKNGYVTGTIPVTVPSNGGSVSVNPPIVVDGPKGNLTGYVKLPGGFNFTDPY
                     SLEVTHPASGIHPPAGVIPASLASGPSQFANAPQYSIFALPAGVWKLKFVSAGYKTVE
                     GIVTIQGNATTNFDIITMVPGSHPPASIGGRALNALNNQPISSLTVRLRPGVGVTTGA
                     YAIDANGATLPAVVTGSNGAFVVANVPAGNYTLEVSGSGYSTTYRTVISAGTDTPAAQ
                     DILVSPVLGANEVRIVLSWNATPKDLDSHLEYGSGWPNQVVWNDRTKLSGDLLLDWDV
                     VTGYGPETVTMKGTVWTQPRRGYSVYNWSRGKNRDTVLTIGQSGATVKVFKSQGLVRS
                     VAAGPSQVSDWWQMFCFSSAQGLIDLGQPGCEVNTFFNVPAN"
     gene            2697..3419
                     /locus_tag="PLG78_00760"
     CDS             2697..3419
                     /locus_tag="PLG78_00760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE21662.1"
                     /translation="MDSEFRLKIPFFERRRRIRLPWTILIFGVIFLSLPVMNYIALGQ
                     RLSIPWKYPRTLLGALNPFEIVLLLLPIVIGVGLLMVKRWAWWLFLAYATTLLAYNAA
                     ILALSRQLYNVNALVQTAIFVSAVFYFLRRDISAPYLRMYPRGWRLQKRKPIVVEVIV
                     DGIRRTTLDVSDTGIYVDWEDCYRSPGEEVHLTFRIEGHEYKCRAGVVRVDEDGAGIA
                     FRGVTASFRKELARDLESLAHG"
     gene            complement(3428..5749)
                     /locus_tag="PLG78_00765"
     CDS             complement(3428..5749)
                     /locus_tag="PLG78_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNE21663.1"
                     /translation="MARNLIIIGGGTLQMPLIETAKAMGLFTIVFDMTLEAPGMKAAH
                     LPVTMSTRDIDGCVREAKRLRETMQIHGVVTAGTDASRAVAAIAGALELPGIRYADAE
                     AASNKVLMRKRLKQQGVPGPDFRSVWSIKEAREAMDELTFPLVLKPADNMGARGVIKI
                     KTRDEIYSAFRHAKKYSPTGEMILEEFMEGPELSIDALAWKGHIRLTGVADRIIAREP
                     YFVELGHNMPSSLPASVLEEAEEVMKAGMRALGIHTGAGKGDIKVARLPAGQKNSRHA
                     TPYDDYAVKVGEIAARLSGGFMSSHTFPLHSGINLHKAAIQIALGDEPSSLEPTRNLV
                     AIERGILSSPGKILELNGVAEMSQVKGIEHIFFTKKTGEIIQEATSNLDKVGHVIAVA
                     PSLEEAEAAAQECLRRVQITVDDTFSVDWKAVEEKARTRFTDQVCWVCKSCVGNCASA
                     VPGMGGVGNMTTFRDNSNALGEIRVIPRYIRGQIQPDTTFEFLGRKFELPIMSAPMTG
                     TATNMNAAISEYDLARILIHGARESGSIAWVGDGATPDRYKTIFRALSEADSFGIAIC
                     KPRADEAALRERFKAAEELGLIAVGMDIDAISFRTMELKKLGATARNYEALRMLRDSV
                     KLPFILKGIMSPEDAEEALRAGVSAIVVSNHGGRVLDDMPGTARVLPEIVRAVGGRIP
                     VLVDGGVRSGQDTFKMLALGASAVLVGRPLGIAAVGGDIAAVKYVFSRYREELARTMN
                     LCGTEKLTEIDSGFIRTYTTEREEADRSSHTEG"
     gene            complement(5824..6768)
                     /locus_tag="PLG78_00770"
     CDS             complement(5824..6768)
                     /locus_tag="PLG78_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNE21664.1"
                     /translation="MNGSQEITWTLHKENPLIAPPWPSPIIADPSLLLPETSPDGKWH
                     LIAHSILGLHSFTSEDGIHWSRPSFLFANAMRPYLYQEGETYYLFYERYRSFQLPFSW
                     VPFPKWKSRIEVRRSKDLKHWSKPHTVLLPHLSWHESKYGKSISNPCLIKVEKIYRLY
                     YSASLERIEDCGFCEPRYIGYAESEQITGPYTSATEPMITPEVSDPHCNLAAGAMKVV
                     EHNGQYLGFQNGIYVDERSVSGSAILLLKSADGITWSRPTEPILKPTSGWMRSHVYAL
                     DVKRRSKTWYLYFNARDDWHWTRGKEKIGLLLGEEAVP"
     gene            complement(6781..8079)
                     /locus_tag="PLG78_00775"
     CDS             complement(6781..8079)
                     /locus_tag="PLG78_00775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE21665.1"
                     /translation="MRIHKICLLSCFFLLCSVIQSLHCETRDSFLVVQTGWTTAADTI
                     SQEELRRSFCAGSVRSSASAWPAASALFGCKGAEPAKVEAFYPASREKLILLDLSETT
                     PRMKALKVDGLSPFADGEKYPLRGKSLPRFTHFILTGVSALTRYTGKSADANGVSVLS
                     EQVRPFFQSADFVHVSNEVSFTSPCVFEKGTRFCSKEAHFQVYRDLKVNVVELTGNHN
                     RDYGNEPFFRTYDWFHAHGMNTFGGGKTEASANEPLVLNANGTRIVLIGFNELCPLNE
                     CAQGSTPGANRFEAEKAHRTVTDIRKKYPDAFVIATVQYGEVSAYKPTESQKRISYAL
                     LDSGADLAYGSQAHQVQQMEFRKGKVILHGLGNFLFDQTHTFGLRQGYFMNLYFQGNR
                     LAGMEPVFTWIDEKFRPVPATPAQAREIKSAIYSDDLLYK"
     gene            8254..10581
                     /locus_tag="PLG78_00780"
     CDS             8254..10581
                     /locus_tag="PLG78_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21666.1"
                     /translation="MTYSEPIELEGEWGFFRNRFLPPTEDAAADRTMTVPGVWPSHGE
                     PGTGFATYRLKVLLPPGLRRPALFFAHTETAYSIYADGRLLGSAGTPGETEATTTPEW
                     RPRVFEIPSDSSEVVLVIHLANFHHARGGIGYGAYIGSMEHITLMRERELGRDAFLVG
                     ILLVLALYHGIIFFLRRKDPAPAYFALLCLNLAARAAFTGSTPILLGLDSIPYNVHAR
                     IVYLSFYLAAPAFAAYIAAIYSDTILIWSQRATVAIALLFAFSVLFLTPLHFTRFIDY
                     YYIVVGLASVFLLFGIARGLFKKVQGVGPVGLGLLLMVAAYANDVLYNKNIINTALLS
                     PYGIIVFMFVQAVGLSKRFSRAFALSESLSENLTRTNAELVSWKNRLEERVAERTREI
                     ETARSIAENSSQAKAEFLAHMSHEIRTPMNGIMGMLRLLDDAKLGEDEQRYLSTIRAS
                     SETLLTILNDILDISKIESGAMTLTDEPVVIKDLITSLTAMTEPAAQRKGLQLLVIFP
                     LNMPQAILTDRTRVYQILLNLLSNAVKFTDSGTITLKVDRDNGMIRFQVKDTGIGIPP
                     DKFGLLFQSFTQLDSSPARRVGGTGLGLAISLQLAHLMGGEITAESIPGGGSSFILIL
                     PERPTTVDTTRVDTPAAPSDLLPEGLQVLAAEDNEINRIVFQKIGERLRMTVDTAEDG
                     LTAVDMSLLKRYDIIFMDIEMPGLDGVGALKKIQEKLGESAPPIVAVTANAMAGVREK
                     LLTAGFKAYISKPYSPEDIVDAVLAVIRPSSVQKS"
     gene            10616..11503
                     /locus_tag="PLG78_00785"
     CDS             10616..11503
                     /locus_tag="PLG78_00785"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21667.1"
                     /translation="MFRSSVLQAIGHSLTTDQMDILAHEVNESFNLGVVSGFGSSIRV
                     PLQVAVRCIGDFFAQDKDLLAFLAHVLAREGKFVAGSQVLLKGKAALLELLEAESWHY
                     DREMRQFVRDQAMERTADWGHMAEGREYHLTFASVDVVGSSQFGHENAQVFAQMREYV
                     RNYAEVWNGRLWNWMGDGGMAAFHGADSVNRCVISMLAVLMNLPVFNIKYLAQGPELR
                     IRVGIHYGTATYKQPVNEIFSSDMRVAQKLESEVSEPNRMAISGSVYTFLKPEMYPCF
                     VAGPRFDHLETYRLTEIPL"
     gene            11500..12426
                     /locus_tag="PLG78_00790"
     CDS             11500..12426
                     /locus_tag="PLG78_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21668.1"
                     /translation="MTHERSTIVIVDDDPLILEVLQRGLASQPDMSVLIFPNGESYLE
                     FFASTKPDLLLLDAGLPGISGLEVLARIRARYSRTELPVIFISSDSESAQVVSVLEAG
                     ANDFVGKPVDLPVLIARMKVHLSLPGSRSPTPSQPSSTFVEGQTYPLAFLYCQVVVDQ
                     KFSVSRQGSEIQSSLGVLFDMYSRTAQRYNGQLWLRKDDAGFFVFHKQDAVSVVMCAI
                     ELMTLNVLHGALQDRSRDFGINIGLDLGDTVYRADPSILQSAALNKSAHLAKEDGRGR
                     LRISQSIWAQLSPAARRYFDEGPDCWIYRSIF"
     gene            complement(12440..12622)
                     /locus_tag="PLG78_00795"
     CDS             complement(12440..12622)
                     /locus_tag="PLG78_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21669.1"
                     /translation="MKTIAIALFAVFAASCGLIDRKIAGYTGYSKICIDGVRYIQFTS
                     GASVKYTKDGRVETCD"
     gene            complement(12769..13959)
                     /locus_tag="PLG78_00800"
     CDS             complement(12769..13959)
                     /locus_tag="PLG78_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE21670.1"
                     /translation="MKRIVILCLFGPAWILARDLEKRPGRFVPRAEVVSVQPEKNRLC
                     AETVEIPSLKGKDASSVRSFFFEKEKNMILLTEDGSEFGTLTIDRVEPGKPHLICGEF
                     ALNAEQSPRPGDLAGVTLQPANYRTQNLRGPLPVSPPERIRNQADKKMMVRVEWDYVV
                     FGQGEDPAMDNFNPHFFDRDPSVTPKVETFYMDEYEVTNREYLFFTRQTGHRLPESWE
                     KTGSYPEGMEDHPFILASYEDAAAYARWSGKRLPTEIEWEMAARGGLKLLRNGTGVNG
                     ITRSPRVFPMGEWKSEVCNTREMWQDEPRTVSVYTLKDRSPYGIVGMCGSAAEWTETW
                     YNPYPGASKFPMNLAGKQFRVIRGGSFHQDRDAARADSRDYGGFESPENQKRAGIRLV
                     ISAK"
     gene            14228..14446
                     /locus_tag="PLG78_00805"
     CDS             14228..14446
                     /locus_tag="PLG78_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21671.1"
                     /translation="MIVSKLESPLYHRQKREGLPGRGYEAPVAANRKSFDQYLLEAFQ
                     GEVVQEGKRFSSGISPLQRENLIKLGRV"
     gene            complement(14427..15044)
                     /locus_tag="PLG78_00810"
     CDS             complement(14427..15044)
                     /locus_tag="PLG78_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21672.1"
                     /translation="MIQTRNIVTVLKQAAEFEKDFARASFVGALQMARHVALEIKHTV
                     TNLSVFHLSYYLASCVLILVALAPWIEYQIDFGTPESVDVGSSAKLIFLLPGLVGLLV
                     ATFELPFRVQAYRISAGLAVLVWIGGLIFPNPVHTSIHAGEFHLRWWLYLYGPLLLAQ
                     GTLSLQGLTEASIHLDRFQDRLFPPPAAPEGKAPSRRKGAKPGRA"
     gene            complement(15056..15505)
                     /locus_tag="PLG78_00815"
     CDS             complement(15056..15505)
                     /locus_tag="PLG78_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE21673.1"
                     /translation="MITKRIQEAGLILPQVNPPIASYVPAVRSGSTIFTSGQLPMKEG
                     QLLMTGIMTASRSLEEAQSAMKQCFLNAIAAALLVAREDEIKGVLKLGAFVASAPDFT
                     DQHKVANGASDLAKLIFGDAGVHARFAVGVASLPVNATVELEVIFSV"
     gene            complement(15545..16012)
                     /locus_tag="PLG78_00820"
     CDS             complement(15545..16012)
                     /locus_tag="PLG78_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNE21674.1"
                     /translation="MKRAILIFLLVSALKAQTTDVADRKVTLYDGQGITELAQKYLGV
                     PYRFGGQSPDGFDCSGLTGYVYKKAGYQLPRDAGDQFKALRPIKTPRPGDLVFFKIDG
                     KSIGHVGIYLGNLRFIHAPSTGKTVSIADMRISYWKTRYAGARTIFGQAEAAR"
     gene            16119..>16397
                     /locus_tag="PLG78_00825"
     CDS             16119..>16397
                     /locus_tag="PLG78_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21675.1"
                     /translation="MRAGVIFFALFLAACNNNPYGQFPPGKVIMSNLPDDPRTLDPAR
                     VGDTQSNAIASNLHDTPFEYYYLKRPLEIKPAMAVSMPRTGAAVSGGTQ"
BASE COUNT         3670 a         4156 c         4275 g         4296 t
ORIGIN      
        1 ggtcgtccat ttactgatgg atctgtcggg tttcattttg ccttcgaagg ttacaacatt
       61 ggaacagcca cgcaggtgaa tctttctccc gcccagtgcg cgtggactcc tgcaacgcag
      121 gcaccagcat ccatcagtgg gcaggtagac aatgcggcta acgtggcggc tgcatccggt
      181 ctgactgtga agctcatttc ttctggcggt tctgtggtgg cgcaaactac tacagacgga
      241 agcggggctt tcactctgtc tgcatcttct gttgccggcg gcatttatcg aattgagatc
      301 tccggctccg cctacaatac tcagaatgct tctttgctct ttgttcctct cgcaaccgga
      361 gcatctacga gtgtgaatgc tggatccata tctgtgattc ctaaggcttc taacattgcc
      421 ggaaccattc gggatgcggc tacaagcgcg ggcattgcag gtgccgtcat tagctttaga
      481 ccggccgcat ctcagcctgt ttctaagctc acctatcttc ttacagaccc ggtgctcggt
      541 gcggcagccg caagatggat tgcagaaaaa aacggcacgg gaagctattc gacttacctc
      601 acaaagtcgt ataacgggcc tgatgcgggt ggaaatctat acatgactgc ggtggccgga
      661 aagtgggctt actatgtaag cgcggccgga tatcagtctt ctacagccgg agaaatcacc
      721 ctcaatggtg cggacgagac ggcgaacatt tcccttacat cggaccccaa gagatcccgt
      781 atatctggtc aggcggttgt ggtggacact ctgatcgatg gatcaaagca atcttacggt
      841 gctactgtgg ccggctacac gaatcaaggg tatgcccttt ccggtttgat tgtagtcatg
      901 ctaaacaatc gcaacaatgc gggggatgca gtggcacacg tcaccactac ttcagcggcc
      961 ggcacgttta cattcacggc tcagcacgta gttctgcctg caggtttgac agatgattct
     1021 gcccgggtgg cctatgctgc ttctcaattc gcgggcggca atgttacgac ggcgaatatt
     1081 tcgggaactg actctgtttc ggactccatc acaacagatg gaacgaacta ctggttcaag
     1141 tctaacagct attccatcta catcatagac ccgcgcggtc atatgagcgg atctctgacg
     1201 actgccacca acggttctgt cgcaagcaac actcctactg ctgccactac cctgagcgtg
     1261 ggcgcaatcg ccgcgcacct gccccgtggc aaaatttccg gagttgtaac ggatgcattc
     1321 tcgacagagg ctatttccgg agccctcgtt cagctcggac gcgatacaaa tccggatccg
     1381 tctctgattt ctttttcgcc tgctagaaag gattcaaatc caatcgcgaa cgtgagcaga
     1441 ctcagcaatt cagcggacgt tcaggttgct gacgtaacca ctccttcgaa cggatcttat
     1501 agttttgaca atattgatcc aggcgattac gtgctgcaga tttcaaaaaa tggatatgtt
     1561 acgggtacaa taccagtcac agtgccgtct aacggtggtt ctgtgtccgt gaatccaccg
     1621 atcgtcgtgg atggacccaa aggaaatttg accggctatg tgaagcttcc gggcggattt
     1681 aatttcaccg atccgtattc acttgaagtc actcacccgg cgtctggaat tcatccgcca
     1741 gccggagtga ttccggcaag cctggcatcc gggcccagcc agtttgcaaa cgcgccacag
     1801 tattccatct tcgcactacc tgcgggagtc tggaaattga agttcgtttc tgccggatac
     1861 aaaacagtag aaggaatcgt cacgattcag ggaaatgcaa ctacgaactt tgacatcatt
     1921 accatggttc caggatcgca tcctccggct tccattggtg gcagagcact gaacgcactg
     1981 aacaatcagc cgatctcttc tctgactgtg cgtcttcgcc caggagttgg agttacaacg
     2041 ggtgcttacg caatcgatgc gaacggtgct actcttccag ccgtggtgac aggttccaac
     2101 ggagctttcg tagttgcgaa tgttccggcg ggcaactata ctctggaagt atcaggttca
     2161 ggctattcca ctacgtatcg taccgtgatc tctgctggaa cagacacacc tgcagcgcag
     2221 gatattctcg tatctccagt gttaggtgcc aatgaagtga gaattgtttt atcatggaac
     2281 gcaacgccca aagatctaga ctcccacctg gagtatggat caggctggcc caatcaagtt
     2341 gtctggaatg atcgtacgaa gttgagcggc gatctgcttc tggactggga tgttgtcaca
     2401 ggatatggtc cggaaacagt gactatgaaa ggaactgtgt ggacacagcc gcgaagaggg
     2461 tacagtgtct acaactggag ccgtggcaag aaccgtgata ctgtgctcac catcgggcaa
     2521 tcaggcgcga ccgtgaaagt ctttaaatcg cagggacttg tgcgctcagt ggcagctgga
     2581 ccttctcagg tcagcgattg gtggcagatg ttctgtttca gcagtgctca gggattgatt
     2641 gatctgggac agcccggatg cgaagtgaat acattcttta atgtgccggc aaactgatgg
     2701 attcagaatt tagactaaag atacctttct tcgaacgccg tcgcaggatt cgtctgccat
     2761 ggacaatctt gatctttgga gtgatctttc tgtcgctgcc agtgatgaat tacatcgcac
     2821 tgggccagcg actgagcatt ccatggaagt atcctcgcac gttgcttgga gctttaaatc
     2881 cgtttgagat cgtacttctc ttgcttccga ttgtaattgg cgttggacta ctgatggtga
     2941 agcgctgggc ctggtggttg tttcttgcct acgcgaccac gctccttgct tacaatgctg
     3001 ccatcctcgc actttcgcgt cagctttaca acgtgaatgc tctcgtgcag actgcgatct
     3061 ttgtttcagc ggttttctat tttctgcggc gcgatatttc tgcgccttat cttcgcatgt
     3121 atccgcgcgg atggcgtctc cagaaaagaa agccaatcgt tgtggaagtc attgtggatg
     3181 gaattcgcag gactactctc gatgtcagcg atacagggat ctacgtcgac tgggaagatt
     3241 gttatcgatc gccaggcgaa gaagttcacc tgacgtttcg cattgaaggc catgagtaca
     3301 aatgcagggc aggtgtagtc cgagtggacg aagacggcgc aggcattgca tttcgcggag
     3361 tgactgcttc ctttcgcaaa gaactcgcca gggatctaga gtctctcgct cacggctgat
     3421 cgcctggtca cccttccgta tgcgaagatc gatctgcttc ttcgcgttct gtagtgtagg
     3481 ttcggatgaa gccagagtcg atttcagtta gcttttctgt tccacacagg ttcatggttc
     3541 gggccagctc ttctcgatag cgagagaaga catatttcac agcagcaatg tctccgccta
     3601 cagccgcaat tccaagcggg cgtcccacca ggacagcgct ggcacccagg gccagcatct
     3661 taaaggtatc ctgaccgctt cgcactccac catccacgag tacaggaatt ctaccaccaa
     3721 ctgctcttac aatttcaggc aacacacgcg ctgtgcctgg catatcatcc agaacgcgac
     3781 ctccatggtt gctcactaca attgcagaaa ctccagcacg cagtgcctct tctgcgtctt
     3841 ccggtgacat gattcccttc aatataaagg gcagtttgac tgagtctctc agcattcgaa
     3901 gtgcttcgta gtttcgcgca gtggcaccca gcttcttcaa ttccattgtg cgaaacgaaa
     3961 tcgcgtcaat gtccattcca acggctatca gccccagttc ttctgctgct ttgaatcgtt
     4021 cgcgaagagc agcttcgtct gcccttggct tacaaatcgc aattccgaat gaatctgctt
     4081 ctgatagtgc tcgaaagatt gttttgtatc gatcaggagt ggcaccatcg cccacccagg
     4141 cgatgcttcc gctttcgcgt gcgccgtgaa tcaaaatgcg agccaggtca tattcagaga
     4201 tcgcagcatt catgtttgta gcagtgccag tcatgggtgc ggacataatg gggagctcaa
     4261 actttcttcc aaggaactca aacgttgtgt ccggctgtat ttgaccgcga atgtacctgg
     4321 gaatgacgcg aatttctcca agtgcgttcg agttgtctct gaatgtagtc atgttgccta
     4381 caccgcccat tccaggaaca gcgctcgcgc aatttcccac gcatgatttg caaacccagc
     4441 agacctggtc tgtgaatctg gttcgtgctt tttcctctac ggctttccag tccacgctga
     4501 atgtgtcgtc gactgtgatc tgaactctgc gcaaacattc ctgggctgcg gcctctgctt
     4561 cttccagaga cggagcgacg gcaatcacat ggccgacttt gtccagattg ctggttgcct
     4621 cttggatgat ttcgcctgtc ttctttgtga agaagatatg ttcgattcct ttcacctggc
     4681 tcatttccgc tacgccgttg agctcaagaa tctttcctgg agaagataga atgcctcttt
     4741 caatcgcaac gaggttgcga gttggttcca gagaagaagg ttcatctcca agcgcaatct
     4801 ggatggccgc tttgtgtaga tttatgcctg aatgaagcgg gaaagtgtgc gaagacatga
     4861 aaccgcctga caaacgtgcg gcgatctcgc cgacttttac cgcataatca tcgtaaggtg
     4921 tcgcgtgacg tgaattcttt tgtccggctg gaagtctcgc caccttgatg tcgccttttc
     4981 ctgcgccagt atgaatgccg agcgcccgca tcccggcttt cattacttcc tccgcctctt
     5041 caagtacgct tgcgggcaaa gacgaaggca tgttgtgtcc gagttccacg aagtatggct
     5101 cacgagcgat gatccggtca gctactccgg tgaggcgaat gtgacctttc caggcgagtg
     5161 cgtctataga aagctctggc ccttccatga attcttcgag gatcatttct ccggtaggac
     5221 tgtacttctt tgcatgacga aatgctgagt agatttcatc gcgagttttg attttgataa
     5281 cgccgcgcgc gcccatgttg tctgcaggct tgagtacgag tgggaaggtg agctcgtcca
     5341 tggcttcgcg cgcttctttg atggaccata cggatctaaa atcaggacca ggaactccct
     5401 gttgcttcaa tctttttcgc atgagtactt tgtttgatgc tgcttctgca tctgcgtagc
     5461 gaatgcctgg aagttccagg gcacctgcga tcgcggcaac agcccggctt gcgtctgtcc
     5521 cggctgtcac cacgccgtga atttgcatgg tttcgcggag gcgtttggct tcgcgcacgc
     5581 atccatctat gtcgcgtgtg ctcatagtca ccgggagatg cgccgctttc attccagggg
     5641 cttccagggt catatcaaag acaattgtaa aaagccccat agcctttgcg gtttcgatga
     5701 gaggcatttg aagcgtgcca ccgccaatga tgatgaggtt tcttgccatg aaatggtcct
     5761 ccgggtctct tataaaggtc ggcaggaacg gcatttatat gaggagaaat ggcttctggc
     5821 gggttacggg accgcctctt cgcccagcag gaggcctatt ttttctttgc ccctggtcca
     5881 gtgccagtcg tctcgtgcgt tgaagtagag gtaccatgtc ttgctgcgac gctttacgtc
     5941 cagggcatac acgtggcttc gcatccagcc actggttggt ttgaggatcg gctcggtggg
     6001 cctggaccat gtgattccgt ctgccgattt caggagtaga atggcggatc cagagacact
     6061 gcgctcatcc acataaatgc cgttttgaaa acccagatac tgcccgttgt gctccactac
     6121 tttcatggct ccagccgcga gattgcaatg tggatcggat acttccggag tgatcattgg
     6181 ctctgtggcg gaggtgtatg gtcctgtgat ctgttcgctt tctgcatatc caatgtatcg
     6241 cggttcgcag aacccacagt cttcgattct ttccagggat gcactgtagt acaatctata
     6301 aattttttca acttttataa gacatgggtt agagatagat tttccatatt tcgactcatg
     6361 ccatgaaaga tggggtagca agaccgtgtg cggtttgctc cagtgcttca ggtctttcga
     6421 ccttctcact tcaatgcgtg atttccattt tggaaaagga acccaggaga aaggaagctg
     6481 gaaagatcga tatctttcgt agaatagata gtacgtttcc ccctcctgat acagatacgg
     6541 ccgcattgca tttgcgaata gaaaagaggg ccgactccaa tggattccgt cttcggatgt
     6601 gaatgaatgg aggcccagaa tggaatgggc gatcagatgc cactttccgt cgggtgatgt
     6661 ctccggaaga agaagagaag gatctgcgat gataggcgaa ggccagggcg gtgcaatgag
     6721 cggattttct ttatggagag tccaggtgat ttcttgagag ccgttcacaa aacaccgagc
     6781 ctatttgtac agtaaatcgt cggagtagat ggcggatttg atttcgcgtg cttgagccgg
     6841 agtggctgga acaggcctga atttttcgtc aatccaggta aacactggtt ccattcctgc
     6901 gaggcggttc ccttgaaagt ataaattcat aaagtatccc tgtctgaggc caaacgtgtg
     6961 tgtctggtca aacaggaaat ttccgagtcc gtgaagaatg actttacctt ttcgaaactc
     7021 catttgttgg acctgatgtg cctgggaacc gtatgccaga tctgcgccag agtccagcag
     7081 cgcgtagctg atgcgttttt gtgattccgt tggtttgtat gcgctgactt ctccgtactg
     7141 tacggttgca atgacaaacg catccggata cttctttcga atatcagtta ctgtgcgatg
     7201 ggctttttcc gcctcgaatc gattggcgcc cggagtggat ccctgggcgc attcattcag
     7261 cggacagagt tcgttaaaac caatcaatac aattctcgtt ccgttcgcgt tcaacaccag
     7321 tggttcgttt gcggatgctt ctgtttttcc gccgccgaaa gtgttcatgc cgtgagcgtg
     7381 aaaccagtcg tatgtgcgaa agaatggttc gtttccgtag tcgcggttgt ggttacccgt
     7441 aagctcaacc acgtttactt tcaggtctct gtatacctgg aagtgagctt cctttgagca
     7501 aaacctggtt cctttttcaa acacgcaggg agatgtaaaa cttacttcgt tgctcacgtg
     7561 aacaaaatct gcgctttgaa agaatggtct gacctgttcg gagagaaccg acactccgtt
     7621 tgcatccgcg ctcttaccag tgtatcgagt gagcgcgctt acgcctgtta gaataaaatg
     7681 ggtgaaccgc ggtagggact taccgcgaag aggatatttt tcaccgtcag cgaacggcga
     7741 aagaccgtcc actttcagtg ctttcatacg gggagttgtt tctgaaagat cgaggaggat
     7801 gagtttttcg cgcgaagcag gatagaacgc ctctactttc gcgggttctg ctcctttgca
     7861 gccgaacagt gcgctcgctg ccggccatgc ggatgcagaa gagcggacgg atccagcgca
     7921 aaaactgcgt cgaagttctt cctgcgaaat agtgtccgcc gcagtggtcc accctgtctg
     7981 tacgaccagg aatgaatctc tggtttcgca gtggagactc tggatgacgg aacacagtag
     8041 gaagaagcaa gagaggaggc agattttatg gatgcgcaca tgcgattctt gaattccccc
     8101 tcatccattc aagcaacatc cttcttgcgt tccaccggag gacctgcacg atcggttatg
     8161 cggtgtgcga ttttgttcgt tttcattgct gccgcctgtt ctcctcgaac gggtttgcct
     8221 gttgctgcgg gcggcgtcct ggacctgagc aaaatgactt actcggagcc catagagcta
     8281 gagggcgaat ggggattttt tcgaaatcgt tttcttcctc caacggagga tgctgctgca
     8341 gatcggacca tgacagttcc cggagtgtgg ccctctcacg gagaaccggg gacaggcttt
     8401 gccacgtatc gtttgaaagt ccttctcccg cccgggctgc gcaggcccgc tctctttttt
     8461 gctcacactg aaacggctta ctcgatttac gcggatggta gattgctcgg atcggcggga
     8521 actcctggag aaacagaagc aactacaaca ccggagtggc ggccgcgcgt tttcgagatc
     8581 ccttccgatt cttcggaggt ggttcttgtg atccatcttg cgaacttcca tcacgcgcgc
     8641 ggaggaattg gctacggggc ctacataggc tccatggaac atatcaccct catgcgagag
     8701 cgcgagctgg gtcgtgatgc atttcttgtg ggtattttgc ttgttcttgc cttgtatcac
     8761 ggaatcattt tttttcttcg ccgaaaggat cctgcgcccg cttactttgc ccttctgtgt
     8821 ttgaatctgg ccgcgcgtgc agcttttacc gggtcaactc ccattctgct tggactggat
     8881 tcaattccgt ataacgttca tgcaaggatc gtttatcttt ctttctacct ggcagctccc
     8941 gcgtttgccg catacattgc cgccatctat tcagatacaa ttctgatctg gagtcagaga
     9001 gcgaccgtcg ccattgcact cttatttgcg ttctctgttt tgtttcttac tccgcttcat
     9061 ttcaccaggt tcattgacta ttactacatt gtggtcgggc ttgcttctgt ttttttattg
     9121 tttggaattg cgcgcggact gtttaagaaa gtgcagggtg tgggccccgt aggtctgggt
     9181 ctgctgctta tggtggcagc atacgcaaat gatgttcttt ataacaaaaa catcatcaac
     9241 acagctcttc tttctcccta tggaatcatt gtattcatgt ttgtgcaggc ggtggggctc
     9301 agcaagagat tctcgcgcgc tttcgcgttg tctgaatccc tttcagagaa tttgacgcga
     9361 accaatgcag aacttgtgag ctggaaaaac aggctcgaag agagagtggc tgaaagaacc
     9421 agagagattg agacagctcg ttccattgcg gaaaattctt cgcaggcgaa agccgagttc
     9481 ctggctcaca tgagtcacga gattcgcact cctatgaatg gaattatggg tatgcttcgg
     9541 ctgttggatg atgcgaagct cggggaagac gaacagagat acctttctac tattcgcgct
     9601 tcttctgaaa cgcttctgac tattctcaat gacattctgg acatttccaa aattgaaagt
     9661 ggtgcgatga ctcttacgga cgagccggtt gtgataaagg atttgatcac gagcctcact
     9721 gccatgacgg agcctgcagc gcagaggaaa ggtttgcagt tgcttgtgat ttttccgtta
     9781 aacatgccgc aggcgatttt gaccgatcgc acccgcgtgt atcaaatact attgaacctt
     9841 ctttccaatg ctgtcaaatt caccgattcc ggcacaatca ctctgaaggt agaccgcgac
     9901 aacgggatga ttcgctttca ggtgaaagac actggcattg gaataccgcc ggacaaattc
     9961 ggtttgcttt tccaatcatt tacgcaactc gactcttcgc ctgcgcgccg agtaggtgga
    10021 acggggcttg gccttgcgat cagccttcaa ctggctcacc tcatgggagg agaaatcaca
    10081 gcagaatcca ttccaggcgg gggtagctca ttcatactga ttctgcctga aagaccaacg
    10141 accgtggaca ctactagagt ggacactccc gctgctcctt ccgatctgct tccggaaggt
    10201 cttcaggtgc tggctgcgga ggacaacgaa atcaatcgga ttgtgtttca gaagattgga
    10261 gagcgtctgc gcatgactgt ggatacagca gaggacggct tgactgcagt ggatatgagc
    10321 ctcttgaagc gctacgatat catcttcatg gatattgaga tgcctggtct cgatggtgtt
    10381 ggtgcattga aaaaaatcca ggaaaaactg ggagaatctg ccccgccaat tgtggcggtc
    10441 acagccaatg caatggccgg ggttcgagag aagctgctca ctgccggttt caaggcatac
    10501 attagcaagc cctattcgcc agaagacatt gtagatgcag ttcttgctgt aattcggcct
    10561 tcttctgttc aaaaaagttg actgcaattg cgggcgcttt tttattgtgt ctctcgtgtt
    10621 tcgttccagc gtattacagg ccataggtca ttctcttact acagatcaga tggacatact
    10681 cgcccatgag gtgaatgaat cgttcaacct gggagtggtt tccggttttg gatcgagcat
    10741 tcgtgtaccc cttcaggtcg ccgtgcgttg tatcggggat ttctttgcgc aggacaagga
    10801 tttgctcgca ttccttgctc atgtactcgc acgcgaaggg aaatttgttg caggttctca
    10861 agtattacta aaaggaaaag cagctctcct cgaattgctc gaagcagaga gctggcacta
    10921 cgacagagag atgcgccagt tcgtgcgaga ccaggcaatg gagcgcactg cggactgggg
    10981 acacatggca gagggccgtg agtaccatct gacttttgct tcggtagatg tagtgggcag
    11041 ttcgcagttc ggtcatgaga acgcgcaggt atttgcacag atgagagagt acgtgcgaaa
    11101 ctatgcggag gtgtggaacg gtcgtctctg gaactggatg ggagacggag gcatggccgc
    11161 ttttcacggt gcggattcag tgaatcgatg tgtgatttcc atgctggctg ttctcatgaa
    11221 tctaccagtc ttcaatatca aatacctcgc gcagggcccg gaacttcgga ttcgcgttgg
    11281 cattcactac ggaacagcaa cgtacaaaca accagtcaac gagatcttct ccagtgacat
    11341 gcgtgtggct caaaagctgg aatccgaggt cagcgaacca aatcgcatgg cgatttcggg
    11401 atctgtgtac acttttttga agccggaaat gtatccgtgt tttgtagcgg ggccacgctt
    11461 tgatcatctt gaaacgtatc gcctgacgga gatcccactt tgacccacga acgctctacc
    11521 attgtgatcg tggacgatga tcctctgatc ctggaagttc ttcagcgagg ccttgcttcg
    11581 caaccagaca tgtcggtttt gatttttcct aacggggaaa gctatcttga gttcttcgcc
    11641 tccaccaagc ctgatttgct gctgctcgac gccgggttgc caggtatctc aggcctggaa
    11701 gtgcttgctc gcattcgcgc ccgttattcg cgcacagagc ttcctgtgat ctttatttca
    11761 tcagattcgg aatccgcgca agtcgtgtct gtgcttgaag cgggtgcaaa cgacttcgtc
    11821 ggcaagcctg tcgatcttcc tgtcctcatt gccagaatga aggttcatct ttctcttcct
    11881 ggctcaagga gtcccactcc gtctcagccc tcctctacgt tcgtggaagg tcagacttat
    11941 ccgctcgcat ttctctattg ccaggtggtt gtggatcaga agttttccgt ttccaggcag
    12001 ggttctgaga ttcagtcaag tcttggcgtg ctcttcgata tgtattcgag aaccgctcag
    12061 cgctacaatg ggcagctctg gcttcgcaaa gacgacgctg gcttctttgt gtttcacaag
    12121 caggatgcag tttctgtagt catgtgtgct atagagctga tgacattgaa cgtactgcat
    12181 ggcgctttgc aggatcgcag ccgcgatttt ggaatcaaca tcgggctgga tctaggagac
    12241 actgtttaca gagcagatcc ctctatcctg cagagtgcgg cgctcaataa gagcgctcat
    12301 cttgcaaaag aagacggacg tggccgcctt agaatttccc agtcaatctg ggcgcagctc
    12361 agtccagcag cgcgccgtta cttcgatgaa ggacccgatt gctggatcta cagatccatt
    12421 ttctaggcat tcgcctcaat caatcacacg tttctacgcg gccgtctttc gtatatttca
    12481 cggaagcacc tgaagtgaat tgaatgtaac gaactccgtc aatacagatt tttgagtatc
    12541 cagtatatcc ggcgatcttg cggtcaatga ggccgcagct tgcggcgaag actgcgaaaa
    12601 gagcaattgc aattgttttc atgagaactc ttttcaaaca actattcgcc gcgcaagcga
    12661 ttatactgcc ccttgcatag gcgagcgaaa gatttgccgg gccaaacatg gatgaggcga
    12721 tccgctgcga atgccggcgc gcacaattcc ttattgaatg atactcgact attttgcaga
    12781 gataacgagc cgaatccccg ctctcttttg gttttcagga gattcgaatc cgccgtagtc
    12841 gcgagaatcc gcgcgggctg catctctgtc ttgatgaaac gaaccaccgc gaatgactct
    12901 gaattgctta cccgctagat tcattggaaa tttgctagca cctggatatg gattgtacca
    12961 cgtctctgtc cactcagcag cactgccaca cattcccacg attccgtagg gacttctgtc
    13021 tttcagtgta tacaccgata cagtgcgcgg ttcatcctgc cacatctctc ttgtattgca
    13081 gacttcgctt ttccattctc ccatggggaa tacgcgcggg ctccgcgtga ttccatttac
    13141 tccagtgccg tttcgaagca gttttagtcc accgcgcgct gccatttccc attcaatttc
    13201 tgtgggcaat ctctttccac tccatcgcgc gtaagcagct gcatcttcgt aactcgccag
    13261 tatgaaagga tggtcctcca ttccttcagg atacgatccc gttttttccc aggactccgg
    13321 caatctgtgt cctgtttgac gtgtgaagaa aagatactcg cgattcgtta cttcatattc
    13381 atccatatag aaggtttcca cttttggcgt cacggaagga tcgcgatcaa agaagtgtgg
    13441 gttgaaattg tccatggcag gatcttcgcc ctggccaaac acaacgtagt cccattccac
    13501 tcgaaccatc atcttcttgt ccgcctggtt tctgattctc tcgggaggcg aaacgggcaa
    13561 gggaccgcgc aaattttgag ttctgtagtt agcaggttgc agtgttacac cggcgaggtc
    13621 tcccggtctg ggcgattgtt ccgcgtttaa tgcgaattcg ccgcagatga ggtgaggctt
    13681 ccctggttcc acgcggtcaa tggtgagggt gccgaattcg gatccatctt ctgtgagcag
    13741 aatcatgttc ttttcttttt caaaaaagaa ggacctgaca gaagaggcgt ctttgccctt
    13801 gagagatggg atctccacgg tctccgcaca gagtctgttt ttctcaggct gcacggaaac
    13861 cacttctgct ctgggtacga atcgcccggg gcgcttttcc aggtctcggg ccaggatcca
    13921 ggcgggacca aacaggcaca gaatgacgat tcgcttcatt ttcttagaat cggcccgcca
    13981 gttccaattt tttgggtttt ttgcaaaggg gggaggtttt tgtcccaggg ggcgggtaaa
    14041 aagatacaca agcgagaaga aaaaaagagg cgcccaacac aagaatgctt gacttttgtt
    14101 taggtattct ttgtatagtg ttgtcgggca aaaaactctt gtattttagg ctcgtgggta
    14161 gtaaattttg tatgagacat aaactttgtc gggaaaaggc cgtcaaagga tgtgtgtgga
    14221 ggccagtatg atcgtaagca agctcgagag tcccctctac catcgtcaaa agcgggaagg
    14281 gcttcccgga cgtggctatg aggctcctgt tgcagcaaac aggaagagtt tcgatcagta
    14341 cctgctagaa gcgtttcagg gggaagtggt ccaggaaggc aaaaggtttt cttccgggat
    14401 cagtcctctc cagagggaga acctgatcaa gctcggccgg gtttagcgcc tttgcggcgg
    14461 gagggggcct tcccctccgg cgcggcgggc ggcgggaaca acctgtcttg aaatcgatca
    14521 agatggatgg aggcctctgt cagcccttgc agtgagagag ttccctgggc caggagaagc
    14581 ggcccgtaca aatacaacca ccatctcaag tgaaattcgc ctgcatgaat gcttgtgtgg
    14641 actggattcg ggaaaatgag cccgccaatc cagaccagaa ctgcgagccc cgcactgatt
    14701 ctgtaggcct gcacccggaa tggcagctca aaggtggcca ccaacaatcc aaccagacca
    14761 ggcaagagaa atatgagttt tgcagatgaa ccaacgtcta cagattctgg cgtcccaaaa
    14821 tcgatctgat actcgatcca cggtgccaga gccactagaa tcagaacgca ggacgcaagg
    14881 tagtagctca gatgaaacac gctcagattt gtcactgtgt gtttgatttc taaagcaaca
    14941 tgccgcgcca tttgtaacgc ccctacgaag gaagcgcgag cgaagtcttt ttcaaactcc
    15001 gcagcctgtt tgaggactgt gacgatgttt cttgtctgaa tcatcagctc cccgctcaga
    15061 cggaaaagat cacttccagt tccacggtgg cgtttaccgg gagggaagca actcccactg
    15121 cgaatcgggc atgaacgcct gcatctccaa agatgagctt tgcaagatcc gacgcaccgt
    15181 ttgctacttt gtgctgatct gtaaaatctg gcgcgcttgc cacaaaagcg cccagcttga
    15241 gcacaccttt gatttcatct tccctggcga ctagaagtgc ggccgcgatt gcattgagaa
    15301 agcattgctt cattgcactt tgtgcttctt ctagactgcg cgatgcagtc atgatgccag
    15361 tcatcaaaag ctgtccctct ttcataggga gctggccgct ggtaaagata gtggacccgg
    15421 atctgacggc cgggacatac gatgcgatgg gtgggttcac ctgcggaagg atcagccctg
    15481 cttcctggat gcgctttgtg atcacagagc aagaatgcgc agacacagcc tcgcgaaaag
    15541 taatttatct cgcagcttcc gcctgaccaa agatcgtgcg agcacctgcg tacctggttt
    15601 tccagtatga gatcctcatg tctgcgatgc tcacggtctt tccagtggag ggtgcatgga
    15661 tgaatcgtag attgcccaga tagattccaa cgtgcccgat cgacttgccg tctatcttga
    15721 agaacacgag gtctcccgga cgaggagttt tgatggggcg aagcgctttg aattgatctc
    15781 ctgcatccct ggggagctgg tagcccgcct ttttataaac gtatccggtc agaccggaac
    15841 aatcaaagcc atccggcgat tgtcccccaa agcgatatgg tacacccaga tacttttgcg
    15901 caagttctgt gattccctgg ccgtcgtaca gagtgacctt gcgatcggcc acgtccgtgg
    15961 tctgggcctt gagggcagac acgagaagga agatcagaat cgctcgcttc atattcttag
    16021 tatcggacga aatgggatcg aatccagttc gaaccccctt tagaaacgca atcctgcata
    16081 agcagttgcc agcattttct gtggcctgat atcgggtaat gcgcgcaggc gtaatttttt
    16141 tcgcactttt tttggccgca tgcaacaaca acccgtacgg ccagtttcct cccggtaaag
    16201 tgatcatgag caatttgccg gatgatccgc gaacgctgga tccagcgcgc gtaggcgata
    16261 cacaatccaa tgcaatcgct tcgaatctac acgacactcc tttcgaatac tactacctga
    16321 agcgacctct tgaaatcaag cctgcgatgg cagtatcgat gcccaggacc ggggcggcag
    16381 tttctggagg aacgcag
//
LOCUS       DAOQJL010000010        16118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1720_length_16118_cov_4.270373, whole genome shotgun sequence.
ACCESSION   DAOQJL010000010 DAOQJL010000000
VERSION     DAOQJL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_16118_cov_4.270373"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PLG78_00830"
     CDS             complement(<1..137)
                     /locus_tag="PLG78_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE21676.1"
                     /translation="MIRYIEHPTSEEFDLAVIGGGISGACVAYEAASRGLSVALFEKN
                     D"
     gene            complement(139..825)
                     /locus_tag="PLG78_00835"
     CDS             complement(139..825)
                     /locus_tag="PLG78_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE21677.1"
                     /translation="MGTEILSFYRFEDQKDPEGCARALHDPAQERNLRGTVLTAGEGW
                     NVTLFGESADLDGYLSDLSGNAHEPEALEIKRMPLEGNPFRRLMYKVKSEIIKIGVPV
                     DVSEGRGTFITPQQLHNMLENHPEEVLMLDVRNAYEKQAGTFINAVDYNIETFSDFPE
                     AARQKPLPADRTVVTFCTGGIRCEKAVPYLRSIGYTDVLQIEGGIWRYLEKYPAGFFQ
                     GGCFWFDERE"
     gene            complement(828..1442)
                     /locus_tag="PLG78_00840"
     CDS             complement(828..1442)
                     /locus_tag="PLG78_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6521/LA_1396 family lipoprotein"
                     /protein_id="HNE21678.1"
                     /translation="MKLSFLRVCALIFLGAVIVDCRSTVTRTYTKPGLENAELKYVKR
                     VGIVVDKASVAPVHADLRDRMLSYRTREHLTHHSEYIVYPVKTPGPVVDVCKANKKLN
                     GVILLRVLEEKKVGGDLSLNLMSTLLDCKSGDRLWEAEGKRNFEMNDPDFGTLVSGDV
                     KRFGNDARTYSAPFYRITGALYEKMPMPELTDSERIEKIDADAD"
     gene            complement(1444..2049)
                     /locus_tag="PLG78_00845"
     CDS             complement(1444..2049)
                     /locus_tag="PLG78_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE21679.1"
                     /translation="MKQLSIAVTIFLFAFSIHAEEESEARVIAPIKKLIGSVRYSEKA
                     STSAEKKTLEDRALEYIGSAQMADFLLGDFSAKATPEQKEKFASQIREYTRYKAFPLV
                     NKYFKDIDLTYEPPVINGDKARVQSSVVYAGSERLTFSWVLTRVGDNYVITDFLDEKG
                     TSTMQTNKDRQVQPTLKAKGIPGLLATMEGAVNQVKKAAGR"
     gene            complement(2124..2897)
                     /gene="cmoB"
                     /locus_tag="PLG78_00850"
     CDS             complement(2124..2897)
                     /gene="cmoB"
                     /locus_tag="PLG78_00850"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HNE21680.1"
                     /translation="MNYTVEAGVVTLGPPESELDSLRQRALSLGPWRKGPFRVGGLLI
                     EGHWKSDLKWNRAIRAAGDIRGKTVIDVGCNNGYYLFRMREAGASHAIGFDPVAQFEN
                     QFLFLNEFARDPSIDFIRSGYQEMGGATADVVFCMGVIYHQKNPMDVLEKLLGALRPG
                     GRIILETMGLDIGENCLVPSGRYAGSSGIWFVPGPGAVANWCKRTGLKCTLADVWDAR
                     DEQQKTQFAELPLTTDFYHGEKTSEGYPLPLRYLFLVER"
     gene            complement(2894..3814)
                     /locus_tag="PLG78_00855"
     CDS             complement(2894..3814)
                     /locus_tag="PLG78_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNE21681.1"
                     /translation="MDAVGQLVFTAFAIYVLIKFALSIRIVPAQQVFLVERLGKFHKQ
                     LGAGFHSMIPFLDRVTYKLNLKEEAIDVPAQTCFTGDNVQIQVDGVIYMKVEDPYRAA
                     YNVTNYHYALIQLAQTTMRAALGHLELDKTFEERESLNAQIVKVVDEAAEPWGINIMR
                     YEIQNITPPKSILQSMEKQMTAEREKRATIARSEGEKTARINTSEGLKQELINRSEGE
                     KQRTINQAEGQAAEIRAIALATAAGLKKVADAVAAPGGAEAMRLSLVQEYLRMMEGLA
                     KSDTKVVLPVNLADLKETLGGLGLKSKAQE"
     gene            complement(3825..4769)
                     /locus_tag="PLG78_00860"
     CDS             complement(3825..4769)
                     /locus_tag="PLG78_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE21682.1"
                     /translation="MMFLAAVGLILFLLFLKLTLVIVPHQHAYIKERLGSFAGVLNSG
                     WHILIPIADRIAYKHLLMEEVIDVPPQICITKDNVQVEVDGILYIRVLDPKLASYGIQ
                     DYKFASIQLAQTNMRSEVGKLDLDRTFVEREKINDAIIRSLDHASEPWGVKVVRYEIK
                     NIIPPKTILVTMEKQMTAERDKRADIAHSEGERAARINHSEGEKLEAINLSEGEKQKR
                     INEAAGNAAQIELVASATADGLREVAGAMQHPSGARAVQMRIVEDYIKQFGAVISQAQ
                     TSVVPVGLANIQGIMAGMSELLSKGQVVTPPPAPSKDA"
     gene            complement(4822..5256)
                     /locus_tag="PLG78_00865"
     CDS             complement(4822..5256)
                     /locus_tag="PLG78_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNE21683.1"
                     /translation="MHWLVWIVGGLVVAFAELAIPGLIALFLGGGAVVTGVLVYFGWL
                     DSISSQLSAWICISLALFLLFRDQVMKRFPSLEKRDAITEDQVMIGKIVPVIEQITPE
                     KPGRVSVHQSSWKAVSSSTIEKGENARIIARDQLVLTVEKAN"
     gene            complement(5256..6458)
                     /gene="ftsZ"
                     /locus_tag="PLG78_00870"
     CDS             complement(5256..6458)
                     /gene="ftsZ"
                     /locus_tag="PLG78_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE21684.1"
                     /translation="MMQLVEDGTSPALIKVVGVGGGGMNAVNRMIDAHLRGVEFLVIN
                     TDEQVLRKSRVETRIAIGQKTTRGMGAGGDPEIGLRAAQEDRDRLLQSLKGADMVFIT
                     AGMGGGTGTGAAPIIAEVARELKALTVGVVTVPFEVEGKRRRELALKGLAALRDKVDT
                     LIVIKNDSIFKVIDQNTPVDVAFRIVDEVLLGAVRGISDLVNEAGLINVDFADVRSVM
                     AEKGEAVMGAGEGMGENRVVEAVSQAINNALLEEHSVEGATGVLINVCGGEDMTMKEW
                     RDVSELITQRVDPGANTIIGMTIDPALRDRIRVTVIATGFKRNAAQGAPRKNSIFPES
                     KKPEEPPVRRPYFPAATAAPLKKAVPDEHLAEVQAEPEAEEIPFSIGGKIDLNNMSVP
                     AYLRKQQR"
     gene            complement(6530..7747)
                     /gene="ftsA"
                     /locus_tag="PLG78_00875"
     CDS             complement(6530..7747)
                     /gene="ftsA"
                     /locus_tag="PLG78_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNE21685.1"
                     /translation="MKEFASIDIGTSYTRVLMGRMGDDGVIQINGAGVSPSQGVRNGG
                     VVNIEQTVQSIQTAAREAELMSGLVVEEAVVNITGKHLKGENSRGVVAVTNRDRVVTE
                     SDVLRVIEGAQNIRIPADQEILHVLSREFVVDDQSGIRDPIGMTGVRLESEVHIVTAG
                     KTALTNLRKAVNAAGIRVTNLVMSSLASSEAVLTSGEKDLGVAVVDIGSGVTDIIVYV
                     DGGVFSSATLPWGGSHVTQDLSFGLKIPMEAAELVKRNSGCVQIAAVDPIEKIELPAM
                     SGRPPRWVLRQEIAAIMEPRMREIFELVDQELMRSGKKNALSGGVVITGGGSRVEGAA
                     SLAEEVFSMAVRKGNPLPLGGFFDRVDGPEFATSVGLLIGAAQSTDTARKAPRESGSG
                     WMQKLRSWVSENL"
     gene            complement(7744..8466)
                     /locus_tag="PLG78_00880"
     CDS             complement(7744..8466)
                     /locus_tag="PLG78_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNE21686.1"
                     /translation="MLEQNSSRRQVYVVAGLVSVLLIGAGTGLVFYYGKTYTNVKHIR
                     VAGNVMLSASEVLAAAGIDLARPFTRGDMYSAQNRLLSHPAVASATLQEEGEEVIIRI
                     RERACLAVLRVDGSMIDVDETGRILFYDGRCSASPLVSGAFRIQDNRVHGEGMADVLS
                     ALRALRDGRPDLENRVSEIHLRKGYYTIYVVRPAIQIDVPDLSQENIERITASLAFFE
                     KEGQHRGRIDLRGKNALYLPKT"
     gene            complement(8542..9861)
                     /locus_tag="PLG78_00885"
     CDS             complement(8542..9861)
                     /locus_tag="PLG78_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNE21687.1"
                     /translation="MKVYFIGMGGSGMLPLARLAREFGHTVAGSDPRGAADLDVFSNV
                     DPSRIDAFDSVVYSSAIPPEHPELQYARSRGEFVKVYHRMDFLTELMRYSEVRIGIAG
                     THGKTSTTSLLGWVLLDQGLDPTIFAGGKPLYLKEGMRKGNGPAVFETDESDGSFLRS
                     NANFKICLNVDEDHLEHYGTYRHLIDAFRSFASSGTVVLNGLDSSLPNIPGAIYFAAL
                     EAGQESRADYTGRFLDETDALVVSARGTEVGQLSIPLPGRHFASNALGVFALLDTMIQ
                     QGLLSGLNRKGILESMSRFPGVERRMERIGTAQGSPVFDDYGHHPSEIAAVIRALRVR
                     YPDKRVKIVFQPHRYTRTRDHASAFAEVLKLADEASILPLYSAGEKPIDGIDSEMIAR
                     QTQARLIQEADIPELLDRAGPGDVLLFQGAGDISAMVRRAIASRKST"
     gene            complement(9858..10892)
                     /locus_tag="PLG78_00890"
     CDS             complement(9858..10892)
                     /locus_tag="PLG78_00890"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNE21688.1"
                     /translation="MRVLIVAGGTGGHISPGVALVQEFVRRGVETAILTLRKNEKYPD
                     IGSAGVPVLLYNAPPFPSSILSLIRYPFKMLGAFLYAFRALRTFDCVIGLGGYPTIPG
                     LLAALTRRKQIYLCEQNAVPGQVTRLFSRFASAVFLSFPIERRIPRSELVGNPVRSQL
                     IANRKRNAQNGLILVLGGSQGARQINQMLTALLLDESRPWTKEATWLIQCGESHLESM
                     REMLGARDNVTLFGFHSSIQELYAKAAVLISRSGAGILTEGTLFGLPLVLIPLPTSKD
                     DHQRANARVAEAAGAAITIDQNGADHQRLADVLGKLLTSPEILEDMSKKSAALFAADA
                     ASRIAERVLA"
     gene            complement(10889..11992)
                     /locus_tag="PLG78_00895"
     CDS             complement(10889..11992)
                     /locus_tag="PLG78_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNE21689.1"
                     /translation="MTAKRDVVLFALILLLAGMGLFALHPASSLQARIDQGDGDYYFT
                     RQMVFALPGLVGLFFFAYVPLDFLRRYSLLFLAGCAFLLLLVFVPGIGQKVSSRSESF
                     HRWIAFGPVRFQPSEFAKVSLIIYAAHILGKFNLASPDADLRKLIWPFALMGILLFLI
                     LIGPQYGTTICTLSVLAVMIYLSGFPMLRLMLVGLASLPLIGLGIFLWSYRLERFRVW
                     LDPYSFRHEGGYQLVTAFRAFHDGGLTGVEIGTGFAHRYLTFGHTDFILALFAEDFGI
                     LGLSLLFLLYVAFVWRSVSLLRRVNHPFYFMLGAGCLVMIIVQSLLNIAVVTGVVPTT
                     GVSLPFFSYGGSSLIATFCMTGLLLNACGQVDS"
     gene            complement(12028..13365)
                     /gene="murD"
                     /locus_tag="PLG78_00900"
     CDS             complement(12028..13365)
                     /gene="murD"
                     /locus_tag="PLG78_00900"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNE21690.1"
                     /translation="MDLKGKRILVMGASGKSGRSAALCYAKMEATVLLADADAKELPD
                     LAGVVGIQDMRPRDDAGLLDSSVDLIVTAPGVPLSLDIFSSARERGIPVFGENDLGAH
                     LISTSWNPPPFVCAVTGTDGKSTTTALIDHMARSVGISSVACGNFGTPLSSLVFDPPV
                     RMLSLECSSFQLEPARFLRAHVAMILNLSQDHMDRYATLEDYLHAKLNVTMNQTAEDL
                     FIAPKWILDRSTTPARKIALESLQYVRDEIWFSGKAVARLADYSLPGAHNRINLNFAL
                     AALENASRRLGISVSAESIRSAIASFRGLPHRMESVVTVRGIEFVNDSKATTVQAVCS
                     GLTAFAGRSVALLCGGYDKGLDYSELAKFEGVVLLPFGEAGPKIARDTGAARVYATLE
                     EALRAGADLCKSQGQGVVYLGPACASYDAYNSYEERGEHFRSLAMGLAKEWNA"
     gene            complement(13365..14471)
                     /gene="mraY"
                     /locus_tag="PLG78_00905"
     CDS             complement(13365..14471)
                     /gene="mraY"
                     /locus_tag="PLG78_00905"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNE21691.1"
                     /translation="MFEFLYSGGHMPGFFRLFGYVSFRALLAGLFAMAFCFLFGAPII
                     AWLRRLKFGEQIRGDGPQSHLSKAGTPTMGGIMVLASMTAACLLFGNLKNTSFVVTLL
                     CTLSLGAIGFTDDYSKVVLKKKGGMNARMKMLLTLAVALFFCLAYNFMVPAPSVSAKI
                     EYSKTGLFLPFIKGQVIDLTPWFALPFWMVVMVGSAHAVNLTDGLDGLAIGNVCIVAA
                     TLGVLAYITGTPRVANYLNVPAADGAHEIAVFLAALVGAGVGFLWFNAAPAQVFMGDT
                     GSLSLGGAIGMSAILLKKEVLLAIMGGVFVAEALSVMLQVASFKSRGKRIFKMAPLHH
                     HFELSGWPETRVVIRFWLIGVILALISLSTLRIQ"
     gene            complement(14464..15858)
                     /gene="murF"
                     /locus_tag="PLG78_00910"
     CDS             complement(14464..15858)
                     /gene="murF"
                     /locus_tag="PLG78_00910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNE21692.1"
                     /translation="MRDFTQAELESLFGPASHAYRAAIGVSTDSRTALPGNLFVCLQG
                     DAFDGHNFVAQALEKGVSAIIASEQKRIALQPVCDQSSVPFYFVSDTLRALQHLAEMA
                     RSSLKCKVLAVAGSNGKTSTKDMLFTGASAVRQNVHATAGNLNNHIGLPLTLTNIPSS
                     AGLVILELGMNHKGELAELSKIARPDHAIVTSIAEEHAEFFENIEEIAEAELEVAAGM
                     NGGILLYHAQSAGQEVARRIAESHGLSLKIFSAESPSVDASGIHFTFRGNRLTNSNYL
                     NLVMAENLTGALELLAAAGFTGEELTAAAVAAHPGAARRFEVFRRVSGGREILLIDDS
                     YNANQASFEAGISSLRAVLPHGRLALFAGEMAELGHKSDEAHAQVGIAAARAGYTMLF
                     ACGRREAGLMASAYMKHKPDGKVVRGEKPAELLLAFSADDYDGILVKGSRRARMDIVS
                     DEIKRTGFMGRSYV"
     gene            complement(15851..>16118)
                     /locus_tag="PLG78_00915"
     CDS             complement(15851..>16118)
                     /locus_tag="PLG78_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21693.1"
                     /translation="KEEPALIRSQIRAGVRVLDRSYVIEVPGRREAIEHAIVLAKEKG
                     NSMVVVAGKGHEQVQILEDRTEEFSDREEILRAVSRITQGKHHA"
BASE COUNT         4030 a         4491 c         3736 g         3861 t
ORIGIN      
        1 aaatcatttt tttcaaacag cgcaacagaa agccccctgc tcgcagcctc gtaggccaca
       61 caggcaccag agatccctcc cccaatcacc gcaagatcaa actcttcgga tgtggggtgc
      121 tcgatgtagc ggatcatgct attctctttc gtcgaaccag aaacatccgc cctgaaaaaa
      181 tccagccgga tacttttcaa gatagcgcca gatgccacct tctatctgga gaacgtccgt
      241 ataaccaata gatctcaagt acggcacagc cttttcgcag cgaataccac ccgtacaaaa
      301 ggttactaca gttctgtcag ccgggagagg tttctgccga gccgcttccg gaaaatcgct
      361 gaaagtttca atattatagt ccacagcatt gatgaacgtc cccgcctgct tctcatatgc
      421 attgcgcacg tcgagcatca gcacttcttc tggatgattc tccagcatat tgtgcagctg
      481 ctgcggagtg ataaacgttc cacgaccttc gcttacgtcc acaggaacac ctatcttgat
      541 gatttctgac ttcactttat acatcaaacg ccggaatgga ttgccttcta gaggcatgcg
      601 cttgatttcg agcgcctcag gttcgtgcgc gtttccagac aaatcggaaa gatatccgtc
      661 caggtccgcc gattcgccga ataacgttac gttccaaccc tctcctgcag tcaggacagt
      721 tccacggaga tttctttcct gggccggatc gtgcagtgca cgcgcgcatc cttccggatc
      781 cttctggtct tcaaaacgat aaaatgataa aatctcagtt cccacgatca atctgcatct
      841 gcgtcaattt tttcaattct ctctgaatcc gtcagctccg gcataggcat cttctcatac
      901 aatgctccgg taattcgata gaagggagca ctgtacgttc gcgcatcgtt tccaaaacgc
      961 ttcacgtcgc cagaaaccag ggttccgaag tcgggatcat tcatctcaaa attgcgtttc
     1021 ccttccgcct cccacaaccg atctcccgac ttgcaatcca gaagagtgga catcagattc
     1081 aaagaaagat ccccgcccac cttcttttct tcgagaacgc gaagtaggat cactccgttc
     1141 agtttcttgt ttgctttgca aacgtcaacc accggacccg gtgtcttcac tggatagaca
     1201 atgtattcag aatgatgagt tagatgctct cgcgtgcgat atgaaagcat gcgatctcga
     1261 aggtcagcat gaacgggggc cacggacgct ttgtccacga cgatacccac tctcttcaca
     1321 tacttgagct ctgcattctc tagacccggc ttcgtgtatg tgcgagtcac ggtggaacgg
     1381 cagtccacta tgactgcccc cagaaagatc agagcgcata cgcgaagaaa ggaaagcttc
     1441 atctcagcgt cccgccgcct ttttcacttg attgactgca ccttccatgg ttgccagtag
     1501 cccgggaatt ccctttgcct tcagagtagg ctgcacctga cgatctttgt tcgtctgcat
     1561 ggtggatgtt cccttctcat ccaggaagtc agtgattaca taattatctc caactcgagt
     1621 aagaacccag gagaatgtga gtctctccga tccggcgtac acgacagaag actgaacgcg
     1681 cgctttgtct ccgttgatga ctggtggttc ataggtgaga tcaatgtctt tgaaatactt
     1741 gttcacgagt ggaaatgctt tgtaacgagt gtattcgcgg atttgagaag cgaacttttc
     1801 tttttgctcc ggggtggcct ttgcagaaaa atccccgagc agaaaatctg ccatctgcgc
     1861 ggacccaatg tattccagag cgcgatcttc tagagttttc ttttccgcac tcgtgcttgc
     1921 cttttcgctg tatcgcacac tgccgatcaa cttcttgata ggtgcaatga cgcgtgcctc
     1981 gctctcttct tccgcatgaa tggaaaatgc gaagaggaaa attgtaactg caattgagag
     2041 ttgtttcata gtgtttggac ggacagactg gatttttggc tcccttattt tagtgccaaa
     2101 gtacctttga caaccaattt cctctacctt tcgacgagga aaagatagcg cagcggcaat
     2161 ggatagcctt cgctcgtttt ttcgccatga taaaaatcag tagtgagcgg aagttcagcg
     2221 aattgtgtct tctgctgttc atctcttgca tcccagacgt ctgccagagt gcatttcagg
     2281 ccagttcgct tgcaccagtt tgcaaccgct cccgggcccg gaacaaacca gatgccagaa
     2341 ctccccgcat aacgcccgga tggaaccaga cagttttcac cgatgtcaag tcccatggtt
     2401 tccaggatga ttcgcccacc aggacgcagc gctccgagta gtttctcgag aacatccatt
     2461 ggatttttct ggtgatagat tacacccatg cagaacacaa cgtctgccgt tgcaccgccc
     2521 atctcctgat agccggatcg gatgaagtcg atcgaagggt ccctggcaaa ctcgtttaga
     2581 aacaggaatt gattttcaaa ttgcgcgacc ggatcaaagc ctatcgcgtg agacgctccc
     2641 gcctctcgca tgcgaaacag gtagtatccg ttattgcagc caacgtcgat gacggtcttg
     2701 ccgcgaatat ctccggcggc acgaatggcg cgattccatt ttaaatcaga cttccagtgg
     2761 ccttctatca gaagaccacc cacgcgaaac ggaccctttc tccagggtcc cagagagagg
     2821 gctctttgcc ggagagaatc aagctctgac tcgggcgggc caagcgttac tacgccggcc
     2881 tccaccgtgt aattcattcc tgagctttgc tcttcagacc aaggccgcct aacgtttctt
     2941 tcaagtcggc aagattcact ggcagaacta cttttgtatc gctctttgcc agaccttcca
     3001 tcatcctcag gtattcctgg acgagactca aacgcattgc ctccgcgcca ccaggagccg
     3061 ccacagcatc cgccactttc tttaatccgg ccgcagtggc tagagcgatc gcgcgaattt
     3121 ccgctgcctg accttccgcc tgattgatcg tacgctgctt ttcaccttca gaacgattga
     3181 tcaattcctg cttgagaccc tctgatgtat tgatgcgcgc cgttttctcg ccttctgatc
     3241 tggcaatcgt cgcgcgcttt tctctttccg cagtcatttg tttttccatg ctctgcagaa
     3301 tggatttagg aggagtgatg ttctggattt catagcgcat gatattgatg ccccatggtt
     3361 ccgccgcttc atccacaacc tttacaattt gtgcattcaa tgattcgcgt tcttcaaatg
     3421 tcttgtccag ttcgagatgg ccgagcgccg ctctcattgt ggtctgtgcc agctgaatca
     3481 atgcatagtg ataattggtt acgttgtagg cagcacgata cggatcttct actttcatgt
     3541 agatgactcc atccacctgg atctgcacgt tatctcccgt gaaacaggtt tgtgcgggaa
     3601 cgtcgatggc ttcctctttc agattgagtt tgtaagtcac tcgatccaaa aaaggaatca
     3661 tagaatgaaa gccggcgccc agttgtttgt gaaatttgcc cagcctttct acaagaaaca
     3721 cctgctgggc cggaacgatc cgaatcgaca gagcaaattt gatcaggacg taaattgcaa
     3781 aagcggtaaa tactaattgt ccaacagcat ccatgatttt ctccttatgc gtccttagaa
     3841 ggtgccggag gtggagtgac cacctgccct ttgctcagta gctccgacat tccggccatg
     3901 ataccctgaa tgtttgccag accaacaggc acaacagatg tctgcgcctg ggatatgaca
     3961 gcgccgaatt gcttgatgta gtcttccaca atgcgcattt gtacggcgcg agctccggaa
     4021 ggatgttgca tcgctcccgc tacttcgcga aggccatctg cagtcgcaga cgcgaccagt
     4081 tcgatctgag cggcattccc cgcggcttcg ttgattcttt tttgcttctc tccttctgac
     4141 aggttgatcg cttcgagttt ttcaccttca gagtgattga tgcgagccgc ccgctctcct
     4201 tctgaatgag cgatgtccgc gcgcttgtcg cgctctgcag tcatctgctt ttccatggtt
     4261 acgaggattg ttttgggcgg gatgatgttt ttgatttcgt atcgaacgac tttcacaccc
     4321 catggttcgc ttgcgtggtc gagactgcga atgattgcat cgttgatttt ttcgcgttcc
     4381 acgaaggtgc gatccagatc cagtttcccg acttcggaac gcatgttggt ctgagccagt
     4441 tgaatggacg caaacttgta atcctgaatt ccgtagcttg caagctttgg atcaagaact
     4501 cgaatgtaga gaattccatc cacttctacc tgcacgttgt ctttggtgat acaaatctgg
     4561 ggtggaacat cgatcacttc ttccatgaga agatgtttgt acgcgatgcg atctgcgatt
     4621 ggaattagaa tgtgccaacc agaattgagc acgccggcaa aactgccgag tctctctttg
     4681 atgtacgcgt gctggtgcgg cacaatcacc agtgtgagct ttaagaacaa tagaaataga
     4741 atcagtccaa cggcagcaag aaacatcata ttctccgaag cgtgttctgt ggacgcccga
     4801 tgaatcgtca attgcttttg gttaatttgc tttttcgacc gtcagaacaa gttgatctct
     4861 tgcgatgatg cgagcgttct cacccttctc aattgtagaa gaagaaacag ctttccagga
     4921 actctgatga acggaaacac gcccgggctt ctccggcgtg atttgctcta tcacaggaac
     4981 gattttcccg atcatcacct gatcttctgt gatggcgtct ctcttctcca gagacggaaa
     5041 acgcttcatc acctgatcgc gaaagagtaa gaagagcgcc agagaaatgc aaatccacgc
     5101 agaaagttga gaagaaatgc tatccagcca tccaaagtag acgagcactc ccgtgaccac
     5161 agcaccaccg cccaggaaca gggcgatcaa tccgggaatc gcgagttccg cgaacgctac
     5221 taccagcccg ccaacaatcc aaacaagcca gtgcattatc tctgttgctt acgaagatat
     5281 gcgggcacgc tcatgttgtt caagtcgatt tttccaccga tagaaaacgg aatctcttct
     5341 gcttctggtt cagcctgaac ttcagccaga tgctcatctg gcactgcttt cttaagcgga
     5401 gcggccgtag ctgcaggaaa gtatggcctg cgaacgggtg gctcttctgg tttctttgac
     5461 tcgggaaaaa tggaattctt tctgggcgca ccctgagctg cgtttcgctt aaaaccggtg
     5521 gcgatgactg taacgcgaat ccggtcgcgc aatgctggat caatggtcat tccaatgatt
     5581 gtattggctc ccggatccac tctttgagtg atgagttcgc ttacgtcgcg ccactctttc
     5641 atggtcatat cttcgccgcc gcaaacgttg atcaaaacac ctgtcgcgcc ttccacactg
     5701 tgttcttcga gaagagcatt gttgattgcc tggctcactg cttctactac acggttttca
     5761 cccatgcctt cgccggcacc catcactgct tctcccttct ccgccatcac actgcgaaca
     5821 tccgcgaaat ctacattgat caggcccgct tcattgacca gatcagaaat gccgcgaaca
     5881 gcacccagga gcacttcatc tacaatgcga aacgcaacat ccactggagt gttttgatca
     5941 atcactttga agatagaatc gtttttgatt acaatcagtg tgtctacttt gtctcgaagt
     6001 gcagccaggc ccttaagagc cagctctcgt cttcgctttc cttctacttc aaagggaact
     6061 gtgaccacac cgacagtcag cgcctttaac tcgcgagcta cttccgcgat gatcggagct
     6121 gcacccgttc cagtgccacc tcccattccg gcggtgatga ataccatgtc tgctcccttc
     6181 agagattgca gcaacctgtc tctatcttcc tgggccgcgc gaagaccgat ctcaggatct
     6241 ccacctgcac ccatgccgcg ggttgtcttt tgtccaatgg cgattctggt ttccaccctg
     6301 gattttctga gaacctgctc atccgtgttg atgaccagga attccacgcc gcgcagatga
     6361 gcatcaatca tgcgattgac tgcattcatg ccgccaccgc cgactccaac cactttgatc
     6421 aatgccggtg atgttccatc ttccacaagc tgcatcatat ctcattcctc tatatatatt
     6481 gtatctattt ctatactttc aaatttcttt atgattgcgt ttctctgttt cacaaatttt
     6541 cggagaccca ggagcgaagt ttctgcatcc atccagagcc agattctcgc ggtgctttac
     6601 gagctgtgtc cgtagactgg gcggctccga tcaaaagtcc cacagacgtt gcaaattctg
     6661 gcccgtccac tctgtcaaaa aatccaccca gaggcaaagg attgcctttt cgcacggcca
     6721 tagagaaaac ttcttcagcg aggctggccg ccccttctac tctggagccg ccacccgtga
     6781 tcacaacacc gccagaaaga gcgttcttct tgccagatcg catcaactcc tggtccacca
     6841 gctcaaagat ctctcgcatg cgcggctcca tgatggccgc aatttcctga cgcagtaccc
     6901 agcgtggtgg cctgccggac atcgcaggca gctcaatttt ttcaattgga tctaccgctg
     6961 caatctgcac gcatcccgag ttgcgcttca ctagctccgc ggcttccatt ggaatcttca
     7021 gaccaaaaga aagatcctgc gttacatggc ttccgcccca gggcaaagtt gcagaagaaa
     7081 agactccacc gtccacatac acaatgatgt ccgtgacgcc cgaaccgatg tccaccacag
     7141 cgacacccag gtctttttcc cccgacgtaa gaactgcttc tgaactggca agagaactca
     7201 ttaccagatt tgtaacacga atgccagctg cgttgactgc cttgcgaaga ttggtgagcg
     7261 cggtctttcc ggcagtgact atgtgcactt cgctttcgag gcgaacgcct gtcattccaa
     7321 ttggatctcg aattccggat tgatcatcca ctacaaattc gcgcgaaaga acatgcaata
     7381 tttcctggtc tgctggaatg cgaatgttct gcgcgccctc tatgactctt agcacgtccg
     7441 actctgtaac aactcgatcg cgattcgtga cagcgaccac tccccgcgag ttttcgcctt
     7501 tgagatgttt gcctgtgatg tttacaactg cttcttctac tacaagcccg ctcatgagct
     7561 cggcttcgcg cgccgcggtt tgtatggact gaacggtttg ctctatattc acaacgccac
     7621 cgtttcgcac tccctgcgaa ggagacacgc ccgcgccgtt gatctggatg acgccatcat
     7681 cgcccattcg ccccatcaaa acgcgagtgt atgaagtgcc tatgtcaatg gatgcaaatt
     7741 ctttcatgtt tttggaagat acagtgcatt tttgccgcga agatcgattc tgccgcggtg
     7801 ctgcccttct ttttcgaaga atgcaaggga tgccgttatg cgctcaatgt tctcctgtga
     7861 caaatccggc acgtcaattt gaatggccgg ccttactacg taaattgtat agtaaccttt
     7921 gcgaagatga atctcagaaa ctcgattttc gagatcaggc cttccatctc gcagtgcgcg
     7981 aagagcggac aaaacatccg ccataccttc gccgtgaact cgattgtcct gaatgcgaaa
     8041 cgctccactg accaggggcg aagcactgca acgaccatca tagaacagaa tccgtcctgt
     8101 ctcgtccaca tcgatcatag atccgtccac tctgaggaca gcaagacacg cccgttcacg
     8161 aattcgtatg attacttctt cgccttcttc ctgtaaggtg gcgctggcaa ctgctggatg
     8221 agagagcaaa cgattctgcg cgctgtacat atcgccgcgc gtgaatggtc tggccagatc
     8281 aatgcccgcc gcagcgagaa cttcggatgc agagagcatg acattcccgg ccacgcgaat
     8341 gtgcttaaca ttcgtatagg tcttgccgta gtaaaaaaca agcccggtgc cggcaccgat
     8401 caacagaacg gatacaagac cagcaaccac gtagacctgt cttcttgagc tgttttgctc
     8461 tagcatgcgt tcggaaaata ttatgtcgca ttgcatgcgc aaccaaaaaa accagtttgc
     8521 cctgaaaggg cctggaaaaa ctcaagtgct cttgcgactt gcgattgctc ttcgcaccat
     8581 agcggagata tctcctgctc cctggaacaa aagaacgtcg cctggccccg cacgatcgag
     8641 caattctggt atatctgcct cctggatcaa acgagcctgt gtttgtctgg caatcatttc
     8701 tgaatcaatc ccatcgattg gcttttctcc cgcagaatag agaggcagaa tagaagcctc
     8761 atctgccagt ttcagcacct ctgcgaaggc agaagcatgg tctctcgtgc gcgtataacg
     8821 gtgaggttga aatacgatct tcactcgctt gtctggatat cgaaccctga gtgcgcgaat
     8881 aacggctgca atttcggaag gatgatgccc atagtcgtca aaaacaggag aaccctgagc
     8941 tgtgccgatc cgctccattc ttctttctac tccggggaaa cgagacatgg attccaggat
     9001 gccttttcta ttgaggcctg agagtagccc ctgctggatc attgtgtcga gcaaagcgaa
     9061 cactccgaga gcattcgatg caaagtgccg accgggaagc ggaatagaaa gctggcccac
     9121 ttccgtccct cgcgcagaca ctacgagtgc atctgtctca tctaaaaagc gcccggtgta
     9181 gtcggccctc gactcctgcc cagcctcgag cgctgcgaaa taaatcgccc ctggaatgtt
     9241 tgggagagaa gaatccagcc cattcaaaac tacggttccg gaagaagcaa aggatctaaa
     9301 cgcatcgatc aaatgacgat aggtgccgta gtgttccagg tggtcttcgt ccacattcaa
     9361 acatatcttg aaatttgcat tggatctgag aaaggaacca tccgactcat ccgtctcaaa
     9421 cacagcgggg ccattccctt tcctcattcc ttctttcaag tagagaggtt ttccgcccgc
     9481 gaaaatggta ggatcaagcc cttggtccag tagaacccag cctagtaagg aagtggtaga
     9541 ggtctttcca tgcgttcccg caattccaat gcgaacttcc gaatatcgca tgagttccgt
     9601 taaaaaatcc attcgatggt aaacttttac aaattctccg cgagaccgtg cgtattgcaa
     9661 ttccggatgc tctggcggaa tcgcagaact gtacaccaca gagtcaaatg cgtctatgcg
     9721 cgagggatca acattcgaaa acacatccag atctgcggct cctctgggat ccgaccctgc
     9781 cacagtatga ccgaattcgc gcgcgagcct tgcgagagga agcatgccgg atccgcccat
     9841 tccaatgaag tacactttca cgctaagact ctctccgcga tgcgcgaagc tgcatccgca
     9901 gcaaacagtg ccgcactctt tttggacata tcttccagga tttctggcga cgtgagcaat
     9961 ttcccgagca catctgccag cctctgatgg tccgctccat tctggtcgat cgtaatggca
    10021 gctccggcag cctcagccac gcgcgcattc gcacgctggt gatcgtcttt ggacgttgga
    10081 agaggaatca gaaccagagg aagtccaaac aacgttcctt ctgtcagaat gcctgcacct
    10141 gatctggata tcaaaaccgc cgccttcgca tacagctcct gaatcgaaga atgaaagccg
    10201 aataacgtca cattgtccct ggctcctagc atttcgcgca tggattccag gtgagactcc
    10261 ccacattgaa tgagccaggt tgcctctttt gtccatggcc tgctctcatc cagtagaaga
    10321 gccgtgagca tctggttgat ctgacgcgca ccctgacttc caccgagtac tagaatcaga
    10381 ccgttctgtg cgtttctttt tcgattcgca atcagctgag atctaactgg atttccaacc
    10441 agttcagaac ggggaatgcg gcgctcgatc ggaaaagata gaaacaccgc actcgcaaat
    10501 ctagaaaaca gtcttgtgac ttgaccagga actgcgttct gttcacacaa atagatctgc
    10561 tttcttcgcg tgagcgcagc gagcaagcca gggatcgttg gatatccgcc cagaccaatc
    10621 acacaatcga atgtccgaag cgctctaaat gcgtacagga atgcgccaag cattttgaaa
    10681 gggtaacgaa tcagagagag tatggaagac ggaaagggag gagcattgta tagaagcaca
    10741 ggaacgccag cggacccaat gtcaggatat ttctcatttt tgcgaagggt gaggatggct
    10801 gtttccactc cgcgtcgaac gaattcctga accagagcaa cgccaggtga aatatgcccg
    10861 ccggttcctc cagccacaat caatactctc atgaatccac ctgcccgcat gcgttgagta
    10921 aaagtccagt catacagaaa gttgcaatca gcgaacttcc accataactg aaaaatggaa
    10981 gactcacgcc tgtggttgga accacgcccg tcacaactgc tatattcaaa agactctgca
    11041 caatgatcat gaccagacaa cccgcaccca gcatgaaata gaaaggatga ttcacacggc
    11101 gaagaagcga aacagatctc cagacaaatg caacatacag tagaaaaagc aaagaaagcc
    11161 caaggattcc gaaatcctct gcaaacaaag ccagaataaa atccgtatgg ccgaatgtca
    11221 gataacggtg tgcaaatcca gttcctatct ccactccagt gagccctcca tcgtgaaagg
    11281 cgcgaaaggc ggtcaccagc tgatagcctc cttcgtggcg aaaggaataa ggatccaacc
    11341 acactctaaa tctttccagt ctatagctcc acagaaagat tccaaggcca atgagcggca
    11401 aggacgcaag accaacgagc atgaggcgaa gcatgggaaa gccggagaga taaatcatca
    11461 cagcgagaac cgaaagcgta caaatggtgg tcccatactg tggcccgatc aaaatcaaaa
    11521 agagtaaaat ccccatcaac gcaaaaggcc agatcaattt acgcaaatcc gcatccggac
    11581 tcgccaggtt gaattttccc aggatatgcg ccgcatagat gatcagactt accttcgcaa
    11641 actcggatgg ctgaaatcgt acagggccaa acgcaatcca tctatgaaaa ctctccgatc
    11701 tggaggatac cttctgcccg attccaggaa caaagacaag caggagcaaa aacgcgcatc
    11761 ccgccaggaa caagagcgaa taacgtctca ggaaatcgag cggcacgtag gcaaaaaaga
    11821 agagccctac gagcccgggc aaagcgaaca ccatctggcg cgtgaagtag tagtctccat
    11881 ctccctgatc aatgcgagcc tgcagcgaag aagcaggatg aagagcaaac aatcccattc
    11941 ccgccagaag caaaatcaga gcgaataata cgacgtcgcg ctttgcagtc acggccgctt
    12001 caatttcgga aaatgagggc ccccacttca agcgttccac tcctttgcaa gacccattgc
    12061 cagagaacga aagtgctccc ctctttcttc ataagaattg taggcatcat acgaagcaca
    12121 ggctggcccg agataaacca ctccctgccc ttgacttttg cacaaatctg cacccgcgcg
    12181 aagtgcttct tcaagagtcg cataaacgcg agcggctcca gtatcgcgcg caattttagg
    12241 gcccgcttct ccaaatggca aaagcaccac tccctcaaat ttcgcaagtt ccgaatagtc
    12301 gagccctttg tcgtaaccgc cgcacaaaag agccacagat cgccccgcaa aagcagtgag
    12361 accggagcac acagcctgca ctgtcgttgc tttggaatcg ttgacaaatt ctattccccg
    12421 cactgtgacc actgactcca ttctatgtgg caatccgcga aaagacgcaa tggcagatcg
    12481 aatgctttcc gccgaaacgc tgatacccag acgccttgat gcattctcca gtgcggcgag
    12541 agcaaaattc aagttgatcc gattgtgcgc gcccggcaaa gaatagtccg cgagtcttgc
    12601 caccgctttt ccagaaaacc agatctcatc gcgcacgtac tgaagagact caagggctat
    12661 cttgcgcgct ggcgtcgtgg atctgtccag gatccatttc ggagcaatga atagatcttc
    12721 cgcggtctga ttcatagtca cgttcaactt cgcatgcaga tagtcctcca gtgtcgcata
    12781 acggtccatg tgatcctgac tcagattcaa aatcatggca acatgagcgc gaagaaaacg
    12841 agccggttcc agttgaaacg aagaacattc cagagaaagc atccgcactg gcggatcaaa
    12901 cacaagagaa gaaagggggg tgccgaagtt cccgcaggcg acgctggaaa ttccaacaga
    12961 ccgggccatg tgatcgatca atgcagtggt agtggatttg ccatccgtac ctgtcacagc
    13021 acatacaaac ggaggcggat tccaggaagt ggagatcagg tgcgctccca gatcattctc
    13081 tccgaatacg gggattcctc tttcgcgtgc gctagaaaag atatcaaggc tgaggggaac
    13141 accaggtgcc gtgacgatga gatccactga ggaatcaagc agtcctgcat cgtcgcgggg
    13201 acgcatgtcc tggattccaa ccacgcccgc caggtccggc aactcctttg cgtccgcatc
    13261 cgccaggaga accgttgctt ccatcttcgc ataacagagc gcagctgatc ttccggattt
    13321 accggacgcg cccatcacca gaattctttt tccttttaga tccattactg gatgcgcaaa
    13381 gtagaaagtg aaatcaaagc cagaatcacg ccaatgagcc agaatcgaat gaccaccctg
    13441 gtctcaggcc agccagacaa ttcaaaatga tgatgaagag gtgccatctt gaagattctt
    13501 tttcctctgc ttttgaagct cgccacctga agcattacgg acaacgcctc tgctacaaac
    13561 acgccaccca tgatagcaag tagaacttcc ttcttgagca aaatcgcaga cattcctatc
    13621 gcaccgccca gagacaacga acctgtatct cccatgaaga cctgtgccgg cgccgcgttg
    13681 aaccacaaaa atcctacacc cgcacctacg agtgcggcca gaaacacggc gatctcatga
    13741 gctccgtccg cggcaggaac gttgaggtag ttggccactc tgggtgttcc ggtgatgtaa
    13801 gcaaggacgc ctaacgttgc cgcaacaatg caaacgtttc caatggcaag accgtccagt
    13861 ccgtctgtga gattcacagc atgggccgag ccaaccatca ctaccatcca gaaaggaaga
    13921 gcaaaccacg gtgttagatc aatcacctgt cctttgatga aaggcaaaaa caatcctgtc
    13981 tttgagtatt caatcttcgc acttacactg ggagcgggaa ccatgaagtt gtatgccaga
    14041 cagaagaaca atgccacggc cagagtgagc aacatcttca tgcgagcatt catcccacct
    14101 tttttcttga gtactacctt gctgtaatcg tccgtaaagc caatcgcacc gagagataga
    14161 gtgcaaagca aagtcacgac gaaagaagtg ttcttcaagt ttccaaacaa gagacaggca
    14221 gcagtcatac tggccaaaac catgatgcct cccatagtgg gagtgcctgc tttgctcaga
    14281 tgactctgcg gcccatcgcc tcgaatctgt tctccgaact tcagtctgcg aagccaggcg
    14341 atgataggag cgccaaacag aaagcaaaag gccatggcaa aaagcccggc gagaagagcc
    14401 cggaagctaa cataaccaaa aagacgaaag aagcccggca tatggccgcc ggagtataga
    14461 aattcaaaca taagacctgc ccataaaccc tgtgcgtttg atctcgtcgc ttacgatgtc
    14521 cattcttgcg cgacgcgacc ctttgacgag aattccatca taatcgtcgg cagaaaaggc
    14581 aaggagcaat tcagctggtt tttctcccct cactactttt ccgtccggct tgtgcttcat
    14641 gtaagcagac gccatcaacc ctgcttcgcg tctcccgcag gcaaaaagca ttgtgtatcc
    14701 tgcccgggcc gcggcaatcc cgacttgtgc gtgagcctcg tcactcttgt ggccgagctc
    14761 tgccatttcc ccggcaaaca gtgcgagtct tccgtgaggc aaaactgcgc gcagagaaga
    14821 aattcccgcc tcgaagcttg cctggtttgc attgtaggaa tcgtctatga gcaggatctc
    14881 gcgcccgcct gagactctgc gaaacacttc aaaccggcgc gctgctcctg gatgagcggc
    14941 cacagctgcc gcagtcagtt cttccccggt aaacccggct gccgcaagca actctagagc
    15001 acccgtcaaa ttctctgcca tcacaagatt caaatagttc gaattggtaa ggcgatttcc
    15061 cctgaacgta aaatgaattc cagatgcatc tacagacgga ctttcagcgg aaaagatctt
    15121 gagagacagc ccatgcgact cagcaattct tctggccact tcctgccctg cactctgtgc
    15181 gtgatagagt agaatgccac cattcattcc cgcagctact tctagctctg cctccgcgat
    15241 ctcttcgatg ttctcaaaga attctgcgtg ctcctccgca atggaagtga cgatcgcgtg
    15301 gtccggccgc gcgattttcg aaagctctgc aagctcccct ttgtgattca ttcctagttc
    15361 tagaatcacc agtcccgcag aagacgggat attggtcagg gtcaatggca gtccaatgtg
    15421 attgttcaaa ttgccggcag ttgcgtgcac attctgcctc acagcggaag cacctgtaaa
    15481 tagcatatct ttcgtagaag tctttccgtt ggatcctgcc actgcgagca ctttgcattt
    15541 caaagaggat ctcgccattt cagcaaggtg ctggagtgcg cgaagagtgt cagaaacaaa
    15601 ataaaacgga acgctggact gatcgcatac tggctgcaag gcgatcctct tctgttcact
    15661 tgcgatgatg gcactgactc ctttttcaag agcctgagcc acaaaattgt gcccgtcaaa
    15721 tgcatctccc tgaagacaaa caaagagatt ccccggcaaa gcagtccttg aatccgtgga
    15781 aacgccaatc gctgcgcggt atgcatgcga tgcaggacca aacaaagatt ccagttccgc
    15841 ctgcgtaaag tcacgcatga tgttttccct gtgtaatgcg cgaaacagcg cgcagtattt
    15901 cttcgcggtc ggaaaactct tctgttctat cttctagaat ttgaacctgt tcgtgccctt
    15961 tcccagcgac tacgaccatg ctatttcctt tttcctttgc cagaacaatc gcatgctcaa
    16021 ttgcttctcg cctgccaggc acttcgatca cgtaggatcg gtcgagcact ctgactccgg
    16081 cgcgaatctg ggaacggatc aatgcgggct cttctttt
//
LOCUS       DAOQJL010000011        15704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1776_length_15704_cov_3.904531, whole genome shotgun sequence.
ACCESSION   DAOQJL010000011 DAOQJL010000000
VERSION     DAOQJL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1776_length_15704_cov_3.904531"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLG78_00920"
     CDS             complement(<1..575)
                     /locus_tag="PLG78_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE21694.1"
                     /translation="MPGHNSIQHAASAALARFGLSEFRPGQLEAIERILSGKDVMVIL
                     PTGGGKSLIYQLPAVMRETGLTLVISPLIALMKDQADSMRAIGVAAEYCNSTQDELEQ
                     RRVLSHCVTGKVKILYVSPERALSGDFLAILPRMNVNLIAVDEAHCISQWGHDFRPEY
                     RALHKLRIPGVPVAALTATATARVTRDVISSL"
     gene            complement(683..940)
                     /locus_tag="PLG78_00925"
     CDS             complement(683..940)
                     /locus_tag="PLG78_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNE21695.1"
                     /translation="MAQCVKCGNNMEKVLVAGGTEIDCCDHHGVWLDLGELDTIVQYY
                     QKKRESSQPGLAGTFIDSAARGAGFGAGASLARGLIQKLLS"
     gene            1028..1627
                     /locus_tag="PLG78_00930"
     CDS             1028..1627
                     /locus_tag="PLG78_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21696.1"
                     /translation="MIRYSPVLFALLALLQQPLHSENSGHSVRNSSFLPAEPFVLNFL
                     YFPSPRNWTWNYAPVDGNHTLVFSLPKNEKLGTSIAIRFREDETGEEFDRFQKVMQDL
                     QKSGAKPPEQLPADILEQTATKKDAFMVYMANGDIEFLTLYYFKNYKQFVAMRLIFSP
                     KPALSSEELSQVREMAFQFIQSVKKIEVVNTMKKSVCEK"
     gene            1690..3510
                     /locus_tag="PLG78_00935"
     CDS             1690..3510
                     /locus_tag="PLG78_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE21697.1"
                     /translation="MLIKRLLVALSFLILASSAQAQTLHFIITADTKDPSIGSSVAVD
                     VANLKTMAGQIADATGMTLKLQLLTGSKFTESSFESMLSNLKPGSNDTVFYYNSSHGY
                     RTSRKSSQWPYIYVPDSSKDWDLGAIHDTLAKKGARLTIVMGDQCNSYSDRAMPKDMP
                     RIGKNPLEKGNYKALFLRTKGSIIVTASSPGEYSWGAAQGGEYTIAWREALADAVTED
                     HASWKKLLNNATRVIHAGTPEKQTPLYKTYSLVEGITSTTTSEVPPLASYTLDGGTFK
                     RLSKDDWVLNWNAKQYNMSTYRPSTARYSYLYDKSNDDWYCVDVVDDKLWYWRKTSKE
                     WEIWREIKRAEKPNLLSYTLDGGTFVQNSANQWTLDWSGKRYVMAPYREGTARYTYLY
                     VKDADDWYYVDKTESKLYYWDKDQDDWRMWRTLKKKVADAGSSSYPALACATEEEMQL
                     HKLIMEYRAKNGLAAIPLSKSLTHVARTHVRDLASSKPAGTCNMHSWSIGNQAWTGCC
                     YTPDHAQSRCMWKKPGELTSFKGNGYEIAYGTSGYSATPAGALAGWKSSSGHNNVILN
                     KDKWGSHPWQSVGVGMYGGYAVVWFAEESDTDGKASVCSE"
     gene            complement(3557..6280)
                     /locus_tag="PLG78_00940"
     CDS             complement(3557..6280)
                     /locus_tag="PLG78_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE21698.1"
                     /translation="MARKNLGDAGSFLIFLLGILLFFSLATYTENSAPTGNLIGRVGT
                     YTSSFLFFLLGRTAFIVPVCLLYMGGFALYRGELVEPFVRLTSMIVLSVTAAIWIGLL
                     SQLSTTGWIGETLGGMLSFAFGRWGGFIVSFGVFIPATLLSTGATMQQAGRLIASLYT
                     KLENRPEKPESGGGISAAGLAGMAGLAGLAYTWLSKNKSAKESAEDDVKFLKKESMGD
                     MPLFERKIESQAPAQTTYPSIVESHEIPRREQSFVSTPASSVSRVSDESARIFASASV
                     GQMRTDSIGAAYDGHFTTDQSRFVFRKNTRPISNSSWIDRLPTAASVISSRAEAMKQE
                     RISLSPGPAVIDLVQVPIMNLEPAALSEEVTHTESVDDFVSTEEAEDAGELSVDHPSL
                     SEPSVAHEQMRSIEKVEEETEAELEEDSTDDATQTLEYLPTGVPSIRRRGGRYSLPTD
                     ILNLSNPIPVQDAADEVKETTEKLEKVLRDFGIPGSVVRTQRGPIITLFEVLLDSGVR
                     LNKILGIEGEIRMHLSVPSVRIVAPLPGKPTVGLEIPNRVRQTVTLGELARKDRDFFS
                     KHRLGIVLGKDISGKNLYLDLTRAPHLLIAGASGQGKSVYLNSAIASLLYKYSPEEVR
                     FIMIDPKMVELKLYEGIPHLLLPVITDPRQAARALAWIIQEMEYRYAHLSRVKCRDIL
                     SYNERMKTQGKTERIPFIVIVIDELSDLMLIAPKEVEDALWRLSQKARAVGIHMIMAT
                     QRPSVDVITPHIKANAAARVAFHVAQKVDSRIILDQAGADALLGGGDMLYKSPDSTGI
                     TRVQAPLITEEEIASIVQETRRYGEAVFIELGTEEEEETMEDDGSVDAELLDQAWKII
                     LESGKPTTSYIQRRMRIGYNRAANIIETLEMKGYLGPAIGTSRREILRKQP"
     gene            complement(6375..6824)
                     /gene="dut"
                     /locus_tag="PLG78_00945"
     CDS             complement(6375..6824)
                     /gene="dut"
                     /locus_tag="PLG78_00945"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNE21699.1"
                     /translation="MARIRIALKQGARIPARSTDGAAGYDVCALLPPDTEIIIAPGQR
                     MLVPTGIFLDIPEGYFVTLRPRSGLALKNGITLLNSPATIDWDYRGELQVILANLGQD
                     PFTIRSGDRIAQFLVEKWERADFEEISVESLTGTSRGAGGFGSTGLA"
     gene            complement(7029..9128)
                     /gene="pnp"
                     /locus_tag="PLG78_00950"
                     /pseudo
     CDS             complement(7029..9128)
                     /gene="pnp"
                     /locus_tag="PLG78_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            complement(9179..9457)
                     /gene="rpsO"
                     /locus_tag="PLG78_00955"
     CDS             complement(9179..9457)
                     /gene="rpsO"
                     /locus_tag="PLG78_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNE21700.1"
                     /translation="MTELQEKKDRTKTLIREFGKNERDTGSTEVQIALLSERIKALTD
                     HFKINTRDHASRRGLLMLVGQRRRLLEYLKGRDQDKYKDLINKLGLRK"
     gene            complement(9531..9806)
                     /locus_tag="PLG78_00960"
     CDS             complement(9531..9806)
                     /locus_tag="PLG78_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21701.1"
                     /translation="MLNSLQIYERLRHARVDTRAAREIASVFGDLMDHQIAGKRDLAE
                     TETRVKAEIKVEIHRAKSETLKWMILWVSGLLAAQTGMIMAAIRFVR"
     gene            complement(9911..11161)
                     /locus_tag="PLG78_00965"
     CDS             complement(9911..11161)
                     /locus_tag="PLG78_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE21702.1"
                     /translation="MRRILLALSIGILWFGSPSCLSRCIGGAHDFKEGSKYSSLSPRA
                     QELIRLAFSDLDAKKLMDYHLHIVSTGTTCKECFINPNFYSSLHPIKRMRFKVYVSAA
                     GVTDLTRTDEQYVERLLALIDNMEEHGRFLILAFDKHYTEQGEVDSESTDFYVSNDYS
                     MQVASRSRFLVPAVSVHPARKDALVELERVAKKGARVVKWLPNAMGIHPDDPSYKPFY
                     AMMKKYDMILLSHAGEERAVDAQEYQELGNPLYLRAPLDAGVKVIVAHCASLGQAQDL
                     DAPGHPLVSNFDLFVRLFSEKKYTGRLFADISAMTQFNRLGSHPEPEPLKYILAHPEM
                     HDRLVNGSDYPLPAINIVIRTKALMQNGFISTEERESLNEIYKFNPLLFDFVVKRTLH
                     HPEHADWKLSPGIFLEKKELRPFQ"
     gene            complement(11228..13591)
                     /locus_tag="PLG78_00970"
     CDS             complement(11228..13591)
                     /locus_tag="PLG78_00970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21703.1"
                     /translation="MGLRARITLFTGVVLLIVVSVLLAAVSREQERLFEVRVQRETSP
                     YFLPVRKLVTESAHFQKNMVRLSEARFVNENLDSKLAGKGGLKARFVFNEDYFKTAES
                     RIRSLLPDILEKKGDEWISLSRSAALKKGTPEFADYARYLENRLLLQQNFGEVYLKSL
                     TGLDRRVFRIQALDSAGQIRAAGAGADTQAISDTYNDAWINKLTLNADPPVSALYTIQ
                     SSEVQADGHKFLAFTSAIARNEEIGFRGRALLSAAGTRKLTSLIALDRKYCVALKDAG
                     QKVLAREDALVSQETPVLPAYDAEYKKLSDAYAALLRERQDASVRALKRISADDEESR
                     GALEDAVKNLRGAVLDANLFIRFDYSSYARGYSGREDWRTQFENRYNYMRKWILDGGV
                     EVYYPYAGAGEHLLFNRRNALERMITIDTTPIDDLASQALREESAGFVRIFVDPAKFQ
                     EDRAQERDRILDTALAFGLRIFFVAMLLSAFLVRRIQEIISGARQVGVGNLAVTFETR
                     GSDEVTDLARSLNQMVDGLREREAMRGEMSAAEEIQRRLLPDRMPDNMAEALSFGTFY
                     KAMMGVGGDYFDLMEAGPASMVFCIADVSSHGSGPAIVMTMLRSYLRAAVKRTRDLRK
                     IVLDLNARMFEETPSHMFITMFLGLYDRDKHTIEAVNCGHNKSFIYKYSSGAIEEFES
                     TSLPLGAVDSDLFATAVTTQTIKLSQGDLFFQYTDGLNEGMNAAGEEFGLLRVKDILR
                     ENGRKKPVILLEMIAKAAEEFTGKKIFVPGPSEQKDDIAMIAFRRMK"
     gene            complement(13591..14001)
                     /locus_tag="PLG78_00975"
     CDS             complement(13591..14001)
                     /locus_tag="PLG78_00975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21704.1"
                     /translation="MAAAVHEFKSALEELDRFRAVIDDFVSTDLGEIEKNRVILALDE
                     AFVNVITHGYAQDQNKPIELKMDRKGDGFEFTLLDRAPLFDPLKAVSPDLAGHAESGK
                     NHGLGIHLYTTIMKTTHSARPGGGNILTMFKAAT"
     gene            complement(14003..14341)
                     /locus_tag="PLG78_00980"
     CDS             complement(14003..14341)
                     /locus_tag="PLG78_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE21705.1"
                     /translation="MDLQITSQAAGSVTVLKMVGKLDAQSAPAANDQLKALIGSGKTK
                     VVLDLDGLTYISSAGLGILNANQAEARKAGGMIRLACVKPQVKDVFDLLKFTLLFPMF
                     GTVQDALKDF"
     gene            complement(14343..15263)
                     /locus_tag="PLG78_00985"
     CDS             complement(14343..15263)
                     /locus_tag="PLG78_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21706.1"
                     /translation="MLETGLHKVPVAAAESFAKTVRLLALSGRRAFTTYLVHPLDEAG
                     WKRNAAVDSTKKMLVRIKAASMDPSAVHTVAPHCKRLISQALNETLSAVGDCTIFFLD
                     MMLAHTPVSTAPEAIDFALILDRPLEKFQKESETKNTNLFTDALSSMSKEDFKKAFAP
                     VDLGKTEQQVIVKQEAAILFNKIQIAHRRDDLPACRKLIARYMVQNAESQDQNLSIVR
                     DLIKAFDTRVPGFRAELNQFMAIHLHYDVVAGISQGDIKKAIRGIRKYAHIFQGNPEV
                     PYHMEIDRMEGKLYEIISQKGLWEELKKGE"
     gene            complement(15263..15565)
                     /locus_tag="PLG78_00990"
     CDS             complement(15263..15565)
                     /locus_tag="PLG78_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21707.1"
                     /translation="MRIHLRAFSISLTTIFSLLILILTIWTRLSSSFGSDFMSAFQSI
                     HPNPYGANHSDLTLGEQALGSMFDLFYAATDSLVFALGFGSLYNFLVNKLSKSDSE"
BASE COUNT         3910 a         4096 c         3800 g         3898 t
ORIGIN      
        1 agagacgaaa ttacatctct ggttacgcgc gccgttgcag ttgcagtgag tgcggctaca
       61 ggaacaccag gaattctcag cttatgaaga gcacgatact ccggcctgaa atcgtgtccc
      121 cactgcgaga tgcaatgagc ttcatctact gcaatgagat tcacattcat gcgtggaagg
      181 atggcaagaa aatctccaga taaagcgcgc tccggcgaaa cataaagaat cttaactttc
      241 ccggtaacgc aatggctgag cactcgcctc tgctcgagtt cgtcctgtgt ggaattgcag
      301 tactccgcgg caacgccgat agcccgcata gaatccgcct ggtccttcat gagcgcaatc
      361 agcggcgaaa tgaccagagt cagaccggtt tctcgcataa cagctggaag ctgatagatc
      421 agagattttc cgccgccggt cggcagaatg accatgacgt cttttcccga gaggattctt
      481 tcgattgctt ccagctggcc cggacgaaat tcacttagcc cgaaccgggc gagcgcggcg
      541 gacgctgcgt gctggataga attatgtccc ggcatctgtc cagaataaca ctatacgcat
      601 gttagtcaat tctgttttct tcctgtatgc ttcaaagaaa gcatgccgcg tctttggcgc
      661 ggcctcagac gttaggcgtc gtttagctca acaacttctg aatcaaccca cgcgccaggg
      721 atgctcctgc gccaaagccg gcccctctag cggcgctgtc tatgaatgtc cccgcgagtc
      781 ctggttgcga agattctcgc ttcttctgat agtactgaac aatggtgtcg agctctccta
      841 gatccagcca cactccatga tggtcgcagc agtctatctc tgttccacct gccactagca
      901 ccttctccat gttatttcca cacttcacgc actgagccat tctgcctcct gttgaatcaa
      961 aaaccgcctg agtgacgacg cactcaaatt tgctggaccg cagtttatac atcgtaagat
     1021 gcaagccatg atccgttatt cgccagtcct ttttgctcta cttgctcttt tacagcaacc
     1081 actccattca gaaaattcag ggcactctgt tcgcaactcg tcattcttgc ccgcagagcc
     1141 ctttgttttg aattttttgt atttcccatc tcccaggaat tggacctgga attatgcacc
     1201 ggtagacgga aatcacactc tggtgtttag cctgcccaaa aatgaaaagc tgggaacatc
     1261 cattgccatc cgctttcgcg aggacgaaac gggagaggaa tttgacaggt tccaaaaagt
     1321 gatgcaggac ctgcaaaaat ccggcgcaaa acccccggag cagctgcccg cagacattct
     1381 ggaacagact gccacgaaaa aggatgcatt tatggtttat atggccaatg gagacattga
     1441 atttctaaca ttgtattact ttaaaaatta taaacaattt gttgcaatga gactcatctt
     1501 ttctccgaaa cctgctcttt catctgaaga actttcgcaa gtccgcgaaa tggctttcca
     1561 gttcattcaa tccgttaaga aaattgaggt ggtcaacacg atgaagaaat ccgtctgcga
     1621 aaaatagaaa atcttgtaag ggctctaaaa attgtttgac ccggcacgaa ggatcagcgt
     1681 caaaccttca tgctcatcaa acgactctta gtcgctctat cttttttgat cctcgcatcc
     1741 agcgcgcaag cccagactct gcatttcatc atcaccgcag acacgaagga tccaagcatc
     1801 gggtcaagtg tggccgtgga cgttgcgaat ctaaaaacaa tggccggtca gatcgcagat
     1861 gcgactggta tgacgctcaa actgcagcta ctgactggaa gcaagttcac tgaatcgagc
     1921 tttgaatcca tgctctcaaa tctaaagcca ggatccaatg acactgtatt ttactataac
     1981 tcaagccacg ggtacagaac ctccaggaaa tcttcgcagt ggccatatat ctatgttcca
     2041 gactcttcca aagattggga cctgggggcg atccatgaca cactcgccaa aaaaggcgca
     2101 cgattgacga ttgtaatggg agatcaatgc aacagttatt cggatcgcgc gatgccaaaa
     2161 gacatgcctc gaattgggaa aaaccctctc gaaaaaggaa actacaaggc actctttctt
     2221 agaacaaaag gatccatcat cgtcactgct tccagtcccg gcgaatactc ctggggcgcg
     2281 gcgcaaggcg gagaatacac gatcgcctgg agagaagcgc tcgcagatgc ggtgacagaa
     2341 gaccatgcaa gctggaaaaa gttactaaac aacgcaaccc gcgtcattca tgcaggaacg
     2401 ccggaaaaac aaacaccgct gtacaaaact tattccctgg tagaaggaat cacatcaacg
     2461 acgaccagtg aagtgccacc tcttgcatct tacactctag acggcggcac tttcaaaagg
     2521 ctttcgaaag acgactgggt tctgaactgg aacgcaaagc aatacaacat gtccacgtat
     2581 cgcccaagca cagcgcgcta ctcctatcta tacgacaaat caaacgatga ttggtattgt
     2641 gtggacgtag tggatgataa actctggtac tggagaaaga cttctaaaga atgggaaatc
     2701 tggcgtgaaa tcaaaagagc tgagaaacca aatctactga gctacactct ggacggcgga
     2761 acctttgtac agaactctgc gaaccagtgg actctggact ggagtggcaa gcgctacgta
     2821 atggctccct atcgcgaggg cacggcgcgt tacacatatc tgtatgtaaa ggatgcagat
     2881 gattggtact acgtagataa aacagaaagc aaactctact actgggataa agatcaagac
     2941 gactggcgca tgtggagaac actcaagaag aaagtcgcag acgcagggtc ctccagctac
     3001 ccggcgctag cctgcgccac cgaagaagaa atgcaactcc acaaactcat catggaatac
     3061 cgtgcgaaga atggtctagc tgccattccg ctttccaaat cgctcactca tgtagcgcgc
     3121 acgcatgttc gagaccttgc atcgagcaaa cctgccggca cctgcaatat gcacagctgg
     3181 tccattggaa accaggcatg gaccggttgc tgctatactc cggatcacgc ccagtccaga
     3241 tgcatgtgga agaaaccagg agagctcacg agcttcaaag ggaatggata cgaaatcgct
     3301 tatggcacgt ccggctattc tgcgacgccc gcgggagcgc tggcaggatg gaaaagcagc
     3361 tcaggccaca acaatgtgat cctgaacaaa gacaagtggg gcagtcatcc ctggcaatcg
     3421 gtcggggttg gaatgtatgg gggctacgca gtcgtctggt ttgcagaaga atcggacaca
     3481 gacggcaaag catccgtgtg ctccgaatag attttcgcgc ccgctctaca aaacgggccg
     3541 attctcagat gcgcgatcac ggctgctttc ttagaatttc gcggcgactc gttccgatcg
     3601 cagggcccag atagcccttc atttctaaag tttctataat attcgcagcg cggttgtatc
     3661 caatgcgcat ccttctctga atgtagcttg tggttggctt tccgctttcg aggatgatct
     3721 tccaggcttg atccagaagc tctgcatcca cgctgccatc gtcttccatg gtctcttctt
     3781 cttcctcggt acccagctca atgaacactg cttctccgta tctgcgggtt tcctgaacaa
     3841 tgcttgcgat ttcttcctca gtgatgagtg gagcctgcac acgagtgatg ccagtagagt
     3901 ccggcgactt atacagcatg tccccaccgc ccagaagtgc atccgcgcca gcctgatcga
     3961 gaatgatgcg agaatctact ttttgggcca cgtgaaatgc aacacgcgcg gcagcgttcg
     4021 ccttgatatg aggagtgatc acatccacgg atggacgctg tgttgccata atcatatgaa
     4081 ttccaaccgc acgtgccttt tgcgaaagcc tccacagagc atcttccact tctttgggag
     4141 caatcagcat caggtctgac aactcatcga ttacaatcac aataaatgga attctttctg
     4201 ttttcccctg agttttcatg cgttcattgt aagaaagaat gtcgcggcac ttcactctgg
     4261 acaaatgcgc ataacggtat tccatttcct gaatgatcca cgcaagcgcg cgcgctgcct
     4321 gtctgggatc cgtaatgact ggtaagagaa ggtgcggaat accctcatag agtttgagct
     4381 ccaccatctt cgggtcaatc atgatgaaac gaacttcttc ggggctgtac ttatacaaaa
     4441 gagatgcaat ggcggagttg agatacacgg atttaccctg cccggaagcc cctgcgatca
     4501 gaagatgagg cgcccgagtg agatccagat agagattctt tcctgaaata tcttttccaa
     4561 gaacgattcc gagcctgtgc ttggagaaga agtctctgtc tttgcgcgca agctcgccca
     4621 gggttacggt ctggcgcact cgatttggaa tttcaagacc aacggttggt ttacctggaa
     4681 gtggcgctac aatgcgaaca gatggcacgg agagatgcat gcgaatctcc ccttcgattc
     4741 ccagaatttt gttcaaacga actccagaat caagaagcac ttcaaacaac gtgatgatag
     4801 gaccgcgctg cgtgcgaacc acgcttccag gaattccgaa atcgcgaaga accttttcta
     4861 gcttctctgt agtctctttt acttcatccg ctgcatcctg cacgggaatg gggttagaca
     4921 aattcagaat atctgtggga agagagtatc tgccgccgcg cctgcgaatc gaaggaactc
     4981 cagtaggaag atactcgaga gtttgcgttg catcatccgt ggagtcttct tcgagctcag
     5041 cctcagtctc ttcttcgact ttttcgatgg aacgcatctg ttcgtgtgcg acggagggct
     5101 cactgagaga tgggtgatcc acagaaagct cgcctgcgtc ctctgcctct tctgtagaaa
     5161 caaaatcatc cacagattct gtatgcgtca cttcttcgct gagagccgct ggttccaaat
     5221 tcatgatggg aacctgcacc agatcgatca cagccgggcc cgggctcaga gaaatgcgct
     5281 cctgcttcat ggcttccgct ctagaagaga tcacactggc cgcagttggc aggcgatcga
     5341 tccatgagga attggaaatg ggtctggtat tctttctaaa tacaaaacga gattgatctg
     5401 ttgtgaaatg accatcgtag gccgcgccaa tggagtccgt tctcatctga cccacggacg
     5461 cggaagcgaa aatgcgcgca ctctcgtctg aaacgcgaga gacggaggac gcgggtgtag
     5521 aaacaaaact ttgctcacga cgcggaatct cgtgcgattc tacaatggat ggatacgtcg
     5581 tctgggcggg agcttgcgat tctattttcc tttcaaagag aggcatgtca cccatcgatt
     5641 ctttctttaa aaatttcacg tcatcctccg cggattcttt cgcagattta tttttgctga
     5701 gccaggtgta tgcgagtcct gcaagccccg ccatgcctgc gagaccggca gcgctgatgc
     5761 cgcctcccga ctccggtttt tcaggacgat tctcaagctt cgtatacaga ctcgcaatca
     5821 acctgcctgc ctgttgcatg gtcgcgccag tggaaaggag tgtggcagga ataaaaacgc
     5881 cgaacgatac aatgaagccg ccccatcttc caaacgcgaa cgatagcatg ccgcctaacg
     5941 tctcaccaat ccatccggtg gtagaaagct gagaaagaag tccgatccag atcgccgccg
     6001 tgacagaaag cacaatcatg ctggtaagcc gcacgaaagg ctccaccagc tctccgcgat
     6061 agagcgcgaa acctcccata tagagaagac aaactggaac aatgaatgca gtgcggccca
     6121 ggagaaagaa caagaacgaa gaagtgtaag tcccgactcg accgatcaga tttccagtgg
     6181 gcgcagagtt ctccgtatac gtggccaggg aaaaaaacaa aaggattccc aggaggaaaa
     6241 tgagaaacga tcccgcatcc cccaggtttt tgcgcgccat gccttatttt cggcgtttct
     6301 gcggcagatt gtttctattt ttgcgccccg gaatggagcg agagagcgaa taggccgtcg
     6361 gccagggccc tgcttcaggc caggccagtg cttccaaaac cgcctgcacc ccgactggtg
     6421 ccagtgagcg attctactga aatctcttcg aaatcagccc tttcccattt ttccacgagg
     6481 aactgtgcga ttctatcccc ggatcgaatg gtaaaaggat cctgccccag gttggccaga
     6541 atcacctgaa gttctcctcg gtagtcccag tcaatggtag cgggagaatt gagtagagtg
     6601 atcccattct tcagtgccag gccggacctc gggcgaagag tcacgaaata gccttctgga
     6661 atgtccagga aaatgcctgt aggaacgagc atcctttgcc ccggtgcgat gatgatttca
     6721 gtgtctggtg gaagaagcgc gcaaacgtca tagcctgccg cgccgtctgt agaacgcgcg
     6781 ggaatgcgcg cgccctgttt cagtgcgatg cgaatgcgag ccacaggcga ttacctgtga
     6841 tcgcgtccgc cgcctctcga accgccgccg ccacgaggac cgccgcgtcc gcgatcgcca
     6901 ccgcgaggtg gaccacgatg gccgcgatct ccgccatatc cgccgcgatc acgatctccg
     6961 cggtctcctc cgcgatcgcc accaccttct ccttcatcat aacgagaagg acgagtgttt
     7021 ccagcgccta ccaggtcttt tctacccagt tcaattttgc cctgacgatc tacgcctgtg
     7081 actaccactt ctaccatgtc gccttcgttc atcacgtcgc gcacagagtt aatgcgagta
     7141 ggagccattt tggaaatgtg aaggagtcct tctttgcctg gaaggatttc aatgaatgca
     7201 ccaaaatcag cgattcgttt tacagtgccg ttgaattgcg cacctggctc cacgtctgcg
     7261 aagagatcca tgatgagctt tttggcatac tcgtttgctt ctccgccgga agatgcaatg
     7321 gtaacggttc cgctgtcttc tacattgatt tctgattttg atttttcgat gatcgcacgg
     7381 attacttttc cgcccggacc aatcagttca ccaattcttt ctggatcaat ctggatggtt
     7441 gtgatgcgag gtgcattgac agaaacgctc gagcgcgctt ctgcaagagc cttgttcatg
     7501 attccaagaa tgtgagcgcg gcccttggtt gcctgctcaa tggcagaatc gagaatatcc
     7561 agagtcacac ctgtgagttt gatatcgagc tggaatgcag tgattccttt ttcagttccc
     7621 gcaactttga agtccatatc gccaaagtga tcttctagac ctgcaatgtc agagagaact
     7681 acgaaatcgt ctttatctcc tagaatgagt cccatggcga tgccggacac accgcgcggt
     7741 atgggaacac ccgctgccat caaagctaaa gatccggagc atacggatgc catagaagag
     7801 gagccgttcg attccaggat ttccgataca gtacgaatca cgtacgggaa tgtttcctgt
     7861 ttaggcacca tcactttcaa agcgcgctct gccaggtttc catgaccgat ttcgcggcgg
     7921 cctggacctg agtagcgttt cacttcgcct gtagagaaag gcggaaagtt gtaatgaagc
     7981 atgaactgtt tgctctggag gccactcagg ttttcgtatt tctgagcgtc gtttcctgtt
     8041 cccagagtca caacaccgag ggactgggtc tgtccgcgag taaacactgc agatccgtga
     8101 acagatggga gaacatcgag ttcaacggaa atatcgcgaa tctcatcgag cttgcggcca
     8161 tcgtaacgga ttcctttttt gaaaatgccg tcgcgaacga cttcatattc gagcaaatac
     8221 agatgttctt tgatttcttt gagtgaattg gctatgctct tttcgtcttt tccttctgcg
     8281 cgaagctttg cttcgaaatg agctttggta tctttgatga ctgcatcgat ttcgttctgt
     8341 ctgttcagct tgtcttttgt gctgtttgcc gcagccatct tgtctgtagc aaaagcgtag
     8401 acttcttttt tcagttccag atcaggcagg cgaagcttga tatcgcgctt tgtgcgattg
     8461 actttctttg ccattgcttc ctggagcttg atcttttctt tgatagactt gtgtccaaat
     8521 gcaattgcat ccaggatgtc ttgcttggaa tattcttcgc acacaccttc aatcatcaca
     8581 atgtgagtga gagatccggc cactgtcagc tctatgtctg acttcgcaag aagtgaagga
     8641 cccggatctg caacaaactg cccgtctaca cggccaacaa ctgcagctgc aatgggacca
     8701 tgaaatggaa tgtctgaagt gtgaatggcg gcagacgccg cagtgactgc gtggccttct
     8761 actgcaatgt ctggatccgc agaaagtaca gttacgaata cttgaacttc tgcaaagaat
     8821 ccttccggga acaaaggacg aattggacga tcgatgatac gagagatgag tgtttcatgc
     8881 tcttgaggac gagtttcgcg tttgaagtag ccacctggaa atcttccggc tgcatacgtc
     8941 ttctcgcgat attcaacagt cagaggaaag aaatcctggc cttcttttgc ttctgattct
     9001 ccgcagacag tggcaagcaa caccaggttg cctgcgcgat aaacaatggc gccgtctgct
     9061 tgtttggccc aattgccggt ttcgagtgat acttttttgc catcaattgt ggcttcttct
     9121 ttgaacatag attttcctcc cgagagttac aggcggggcg atgagaagct cggctggatt
     9181 atttccgcag gccgagcttg ttgattagat ctttgtactt gtcttgatcc ctgcctttca
     9241 ggtattcaag caggcgtctt ctttgaccaa cgagcatcag gagccctctg cgcgatgcat
     9301 ggtcccgagt gttgatttta aagtgatcgg tgagcgcctt gatgcgctcg gaaaggagtg
     9361 caatctgcac ctcagtggaa cccgtgtcgc gttcgttctt gccgaattcg cggatcaggg
     9421 tctttgtacg gtcctttttt tcctgtaact ctgtcatttc tcttctttac tctctggttt
     9481 gggccaatct ctggaccctg gccctcaggg aaagcaaaat ccggcgaact ctagcgaaca
     9541 aagcgaattg cagccatgat catcccggtt tgggccgcaa gcagtcccga aacccacaaa
     9601 atcatccatt tcaaagtttc gctctttgcg cggtgaatct ccactttgat ttccgctttc
     9661 acccgcgttt cggtctccgc cagatcgcgc ttgccggcga tctggtgatc catcagatcc
     9721 ccgaaaacag aagctatttc ccgagctgcc cgggtgtcca cccgcgcgtg cctgagcctt
     9781 tcatagattt gaagagaatt cagcatgatt tcttgcattc agagaattct aaagtcgcgg
     9841 tgcgacaagc cttttaggca tcctatttgc cgaaccgcca ggacttccgt gttcgtttcc
     9901 gatcccagct ctactggaac ggcctcaatt ccttcttttc gagaaaaatc ccgggagaaa
     9961 gcttccaatc agcatgctcc ggatggtgca gagttcgctt cacgacaaaa tcaaagagaa
    10021 gaggattgaa tttatagatt tcattgagac tctctctttc ctcggtactg atgaatccgt
    10081 tctgcatgag tgcctttgtt cgaatgacaa tgttgatcgc aggcagaggg taatcacttc
    10141 cgttcacgag gcgatcatgc atttcaggat gagcaagaat gtatttcaaa ggctccggtt
    10201 cgggatgaga tccaagacga ttgaactgag tcatcgcaga tatgtcagcg aataatcgcc
    10261 cggtatattt tttctcgctg aatagacgca cgaacaaatc aaaattggaa acgagcggat
    10321 gaccgggcgc atccagatcc tgcgcctggc ccaggctcgc acaatgtgca acaatcactt
    10381 tgactcctgc atcgagtggc gcgcgaagat aaagaggatt ccccagctcc tgatattcct
    10441 gcgcgtctac tgcacgctct tctcctgcat gcgagagaag aatcatatcg tacttcttca
    10501 tcatcgcata gaagggtttg taggatggat catccggatg aattcccatt gcattgggca
    10561 gccatttcac tacgcgagcg cctttctttg ccactctctc tagctcgacg agcgcatctt
    10621 ttcgcgctgg atgaacagac accgcaggaa ccagaaacct ggaacgagag gcaacctgca
    10681 tgctataatc attggatacg taaaaatcag tactttccga atccacctct ccctgctctg
    10741 tgtaatgttt atcaaaagca agaatcaaaa atcggccgtg ctcttccatg ttgtcgatca
    10801 aagcaaggag cctttctaca tactgctcgt ccgttcgagt gagatctgtc acgccagcgg
    10861 cagaaacgta cactttgaaa cgcattcttt tgattggatg aagagatgaa taaaagttag
    10921 gattgataaa gcattctttg caagtggttc cggtagaaac aatgtggaga tgatagtcca
    10981 tcaacttctt cgcatccaaa tccgaaaacg cgaggcgaat gagttcctga gcacgtggac
    11041 tcagactgga gtacttgctg ccctctttga aatcgtgcgc tcctccaatg catcggctaa
    11101 gacaggaggg agagccaaac catagaatgc caatcgaaag tgccaggaga atgcgtcgca
    11161 cggcgttcga tgacacgagc ccaaaaatgt gtcaagccac aacaatcgat tcccggcccg
    11221 cggaaactta cttcatgcga cgaaacgcaa tcatcgcaat gtcgtctttc tgctcggatg
    11281 gcccgggcac aaagatcttc ttgcctgtga attcctccgc cgctttcgca atcatttcca
    11341 gaagaatcac cggcttcttg cggccgtttt cgcgcaggat gtccttcact cgaagaagtc
    11401 caaattcttc tccggcagcg ttcattcctt cattgagtcc gtccgtgtat tgaaagaaca
    11461 aatctccctg ggaaagttta atagtctgcg tggtgaccgc agttgcgaac aaatcagaat
    11521 ccactgctcc cagtggcaac gaggtgctct caaattcctc gatggcgcca gaactgtatt
    11581 tgtagatgaa ggacttatta tgcccgcagt tcacagcttc gattgtgtgt ttatcccgat
    11641 cgtacagtcc tagaaacata gtgatgaaca tatgagaagg agtttcctca aacattctcg
    11701 cgttcaaatc caacacaatt tttcgcaagt cgcgcgtgcg ctttacggct gcgcgaagat
    11761 acgaccgaag catggtcatt acgatcgcag gtccagatcc gtggcttgaa acatcggcga
    11821 tgcagaacac catactcgca ggccccgcct ccatcagatc aaagtaatct ccgcccaccc
    11881 ccatcattgc tttataaaaa gtgccaaagg agagggcttc cgccatattg tccggcatcc
    11941 tgtctgggag aagacggcgc tggatttctt ctgcagcgga catttcgcct cgcatcgctt
    12001 cgcgctcgcg aagaccgtcc accatctggt tgagtgaacg agctagatcc gttacctcat
    12061 cagacccgcg tgtttcaaat gtgacggcca gatttccgac tcctacctgt cgtgcgccag
    12121 aaatgatttc ttgaatcctt cgcaccagaa acgcactcaa cagcatggcc acaaaaaaga
    12181 tgcgaagtcc aaacgcaaga gctgtatcca gaattctgtc tctttcctgc gctctgtctt
    12241 cttgaaattt agccggatcc acaaagatgc gcacaaatcc cgcggactct tctctgagtg
    12301 cctgagaagc aagatcgtct atcggcgttg tgtcgatcgt aatcatccgc tccagtgcat
    12361 ttcttctgtt aaagagaaga tgctctcccg ctcctgcata gggatagtat acctctacgc
    12421 cgccgtctaa aatccacttt cgcatgtaat tgtaacgatt ttcaaactga gttctccagt
    12481 cttcgcgtcc agaataaccg cgcgcatacg atgagtagtc aaagcggatg aacaaatttg
    12541 catcgagcac ggcgcctctg agatttttga ccgcatcctc gagtgcaccc cgcgattcct
    12601 catcatctgc cgagattcgc ttgagtgcgc gcacagatgc atcctgtctt tctcgcagga
    12661 gtgccgcata cgcgtcggag agtttcttat attcggcgtc ataagcagga agaacaggag
    12721 tctcctgaga cactagcgcg tcttcgcgcg cgaggacctt ctgtcctgcg tccttcaacg
    12781 caacgcagta tttacggtcc agtgcaatga gtgaagtcag cttgcgagtg cctgcggcgg
    12841 acaaaagagc tctaccgcga aagccgatct cttcgttcct tgcgatggca ctcgtaaatg
    12901 caagaaactt gtgtccgtct gcctgaacct cactcgactg gattgtgtac agagcggaaa
    12961 caggtgggtc cgcgttgagt gtgagtttgt tgatccatgc atcgttgtag gtgtcactga
    13021 ttgcctgtgt gtctgcgcct gctccagccg cgcgaatctg gcctgccgaa tccagtgcct
    13081 gaatgcgaaa cacgcgtctg tccagtcctg tcaaagactt cagatacact tcaccaaagt
    13141 tttgctgcaa gaggagccga ttctccaaat accgcgcata gtccgcaaac tcaggagttc
    13201 cttttttcaa agcagccgat ctagaaagac tgatccactc gtctcccttc ttctcgagaa
    13261 tatccggcaa aagactcctg attctagatt ctgccgtttt gaaatagtcc tcattgaaaa
    13321 caaaacgtgc tttgagcccg cctttgcccg caagcttaga atccagattc tcattcacga
    13381 atcgagcttc tgaaagtcga accatgttct tttgaaaatg tgctgattct gtaacgagct
    13441 ttcgcacagg aagaaagtac ggagatgttt ctctctggac tctcacttcg aacaaacgtt
    13501 cctgttctcg cgaaacggcc gccagcagca cagaaacgac gatcaaaaga acgacgccag
    13561 tgaacaacgt tatgcgcgca cgcaatccca tcaggtggcc gccttgaaca tggtgagaat
    13621 gttcccgccg cccgggcgag cagagtgcgt tgttttcata atcgtagtgt agagatgaat
    13681 gccaaggccg tgattctttc ctgactctgc atgtcccgcg aggtccggac tcacagcttt
    13741 cagaggatca aagagaggtg cccggtcgag cagagtaaat tcgaagccat caccctttct
    13801 gtccattttg agttcgattg gcttattctg gtcttgcgca taaccgtgag tgatcacatt
    13861 cacaaacgct tcgtccagag cgagtatgac gcgattcttt tcgatttctc ccaggtctgt
    13921 ggaaacaaaa tcgtcaatga ctgcgcgaaa tcggtccagt tcttccagcg cgctcttgaa
    13981 ctcgtggacc gcggctgcca tttcaaaaat ccttcagagc gtcttgtaca gtgccaaaca
    14041 tcggaaacag gagagtgaat tttagaagat caaacacatc tttcacttga ggcttcacac
    14101 aggccagccg gatcatccct cctgctttgc gtgcttccgc ttgattcgca ttgaggatgc
    14161 ccagtcccgc agaagaaatg tacgtgagac cgtccaggtc aagaaccact tttgttttcc
    14221 cagaaccaat cagtgctttt agttgatcgt ttgctgccgg tgccgattgt gcatccagtt
    14281 ttcctaccat cttcagtaca gttacggagc ctgccgcctg tgatgtaatt tgtaagtcca
    14341 cgttattctc ctttcttcaa ttcttcccac agacctttct gcgaaatgat ttcgtataac
    14401 tttccttcca tcctgtctat ttccatgtga taaggaactt ccggatttcc ctggaagatg
    14461 tgggcgtatt tgcgaattcc gcgaattgct tttttaatgt ctccctgact gattccggca
    14521 actacatcgt aatgtagatg aatggccatg aactggttga gttctgcgcg aaatcctgga
    14581 acgcgcgtat caaaggcctt gattagatca cggacaatgg aaagattctg atcctgactt
    14641 tccgcgtttt gaaccatgta cctggcgatg agctttctgc acgcaggaag gtcatctctg
    14701 cgatgagcaa tctgaatttt attgaagaga atggccgctt cctgttttac tatgacctgc
    14761 tgttctgttt tacccagatc cacgggcgcg aatgcttttt tgaaatcctc tttgctcatg
    14821 gatgagagag catccgtgaa caggtttgtg ttttttgttt cagactcttt ctgaaatttc
    14881 tcaagaggcc tgtccagaat cagtgcgaag tcaattgcct ccggtgcggt cgaaacaggt
    14941 gtatgagcaa gcatcatatc cagaaagaag atcgtgcagt ctcctactgc agaaagagtc
    15001 tcgttgagcg cctgcgaaat gagacgcttg caatgaggtg ccacagtgtg aacagcagaa
    15061 ggatccatgg acgcagcttt gatgcgaacg agcatcttct ttgtagagtc aaccgcagcg
    15121 tttcgcttcc agcctgcttc atcgagtggg tgcaccagat acgttgtgaa tgcgcgcctc
    15181 ccggaaagag caaggagccg caccgttttg gcgaacgact cagccgccgc aaccgggact
    15241 ttgtgcaaac ccgtttcgag catcactcag aatccgattt cgataatttg ttcacaagga
    15301 agttgtacag gcttccgaat ccgagtgcaa agacaaggga atcggtggca gcgtagaaca
    15361 agtcaaacat agaaccaaga gcctgctcgc ccagggtcaa atcagaatga ttggctccgt
    15421 atggattggg atgaatggac tgaaatgcgc tcatgaagtc ggagccaaag ctgctcgaga
    15481 ggcgagtcca gatggtgaga atcaagatta aaagcgaaaa gatagtagtg aggctgatgg
    15541 aaaaagcacg cagatggatt ctcatacgct tcgacatcaa gacgcgcccg cagttgagtc
    15601 aagcccattt ggatctttaa aaatgtgaca ggcgctccag ggagtgtcta tctcaagcgc
    15661 gacgccctgc gcgcagaagg gaaaagaaaa aaggatagat ctcc
//
LOCUS       DAOQJL010000012        15671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1785_length_15671_cov_4.244493, whole genome shotgun sequence.
ACCESSION   DAOQJL010000012 DAOQJL010000000
VERSION     DAOQJL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1785_length_15671_cov_4.244493"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLG78_00995"
     CDS             <1..901
                     /locus_tag="PLG78_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002751910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE21708.1"
                     /translation="KERLLGMLEKLKDRVIGQDHALVAVSEAIQRSRSGLSDPNRPIG
                     VFMFLGPTGVGKTETARALSQFLFDDENAMVRIDMSEYMEKHSVARLIGAPPGYVGHD
                     EGGQLTEAVRRRPYQVILFDEVEKAHPEVFNVFLQIFDDGRLTDSKGRTVDFKNTIIV
                     LTSNIGSHILMEPGSQEEKESRIKKELHNFFRPEFLNRLDDVIFFQPVGLDSLLSIVR
                     IQVDKLVSRAKEQALKIKVDPAVLEWLAKRGYDPQFGARPMKRLIQKDVGNLLSRVIL
                     KGEFNTEKEYTLSLKDDHPVLKG"
     gene            919..1350
                     /locus_tag="PLG78_01000"
     CDS             919..1350
                     /locus_tag="PLG78_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE21709.1"
                     /translation="MQKFIKKILVPVDGSDSSKKALEQAIAVAEAANATITVLEVVED
                     FGPLPGYYEAAPKGEDRVKWISEQRFEKVHPPLDNTKVKWDRKIVEGYPAYEICKVAQ
                     EGSYDLIIIGSRGLNAVTRFLIGSVSDRVVHHAPCTVAVVR"
     gene            1353..2003
                     /locus_tag="PLG78_01005"
     CDS             1353..2003
                     /locus_tag="PLG78_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNE21710.1"
                     /translation="MQDQRSFDEFVEALLGCRKCSTVVGPPVCGSVRSSKIIAIGQAP
                     GPHEARFGRPFAYTAGRTLFKWMASIGISEDVYRANVNMSAVLRCFPGKAKSSSAKSA
                     GDRVPSPAEIENCKEHLEFEFTFLKPRLVIPIGKLAIGVTMPEAARSPLADVIGRAFQ
                     VSAFGARFDCIPLPHPSGLNVWNNVEPGKSLLVKALSLIKEHPAAKELIKKSSAAS"
     gene            2044..2466
                     /locus_tag="PLG78_01010"
     CDS             2044..2466
                     /locus_tag="PLG78_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HNE21711.1"
                     /translation="MKKLIICAVLATAFACSKAPEPEAPKVEAPSKRVFFIEPANGAS
                     VSSPVKVKMGVEGMTVEPAGKIVEGTGHFHIIIDAPASEEGKVVPADDSHKHYGKGQK
                     EADLTLKPGKHKLVLQFADGEHKSYGEKLTDTIEITVK"
     gene            complement(2516..3115)
                     /locus_tag="PLG78_01015"
     CDS             complement(2516..3115)
                     /locus_tag="PLG78_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE21712.1"
                     /translation="MSFRVLTSSTVSKGTTFHTPDHHRHAPVTVSSPALEVMTDLKKT
                     TAITIHPQESLDAAHQKMIHRGVRMLLVVDYNDELVGIITASDTLGEKPIQFVQKNGG
                     QARDLKVSDIMTGQSDIDALSIKDVSNARVGDIIETLKHHHRQHGIVVDNQGPRNEIT
                     LRGIFSLTQIARQLGVQIQTYDVAHNLAELAHFMQKVHP"
     gene            complement(3112..4701)
                     /gene="serA"
                     /locus_tag="PLG78_01020"
     CDS             complement(3112..4701)
                     /gene="serA"
                     /locus_tag="PLG78_01020"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNE21713.1"
                     /translation="MKHTILIADKFDAEGVSKLKAHADLEVIYSGGTKRDELLKIIGN
                     VHGLIIRSATTVDAEVLAAAKNLKLVVRAGVGVDNIDIPEASRHGVVVMNSPGGNSVS
                     TAEHAISLMMSMARNVPQANASTRGGKWEKSKFLGVELTGKTLGVVGLGRIGKEVVKR
                     AKGLRMRVIGYDPFIPPANLANLEIDIVSREEILKQSDFVTVHTPLTETTKDFVSKDN
                     LSQIKKGIRLINCARGGIYNEDALAQGLSDGTIAAAALDVFVQEPVAADHPLLKLENC
                     IVTPHLGASTGDAEFAVAMDTVEQVIDFFTKGIARYALNFPTMEPETLDFLAPYFKGG
                     EKIGRLLTQFLEGDVNSIEIEYAGEIEKFMTDPVKAAILRGALTPALGDEVNFVNAPF
                     LAKDRGIRVTETSEKTCEGYSSAITVKFSSSKTTAGLKFTALQSKPMVVGFQNLPIEF
                     EPEGILLIVQNRDVPRVVGTLGMYLGDQNVNIAHLELGRDSRGGTARCVITTDELLSA
                     DALEKMKRLENILSVVQVDLR"
     gene            complement(4786..5778)
                     /locus_tag="PLG78_01025"
     CDS             complement(4786..5778)
                     /locus_tag="PLG78_01025"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE21714.1"
                     /translation="MVLSILVLLVASIFILGHFGLPEPARPMPEPIDPRQIMPGAEPY
                     RFEGTSGVAFLICHGFEDSPLTTRPLGEFLHGLGHTSIGILLPGHGTKIEDLTRVRFY
                     HWQDYLETLFREERSKYRKVFLVGFSMGGTLMLDLAGRFADTYRPAGVLTISSPVFFN
                     GFFNGKLIIHQPMTMFTGIMRIFTSILRIEGKKKTNSMQRLNPWLGYDATYALDTLHS
                     FKRSLSGVRRVLPRISVPYCSIMAANDRTVSAENQIYIYNNIGSREKRALQFILPPDM
                     TNMHTLLTHKKAQKRVLRFIQEFVDDTLKDFVPETPRRGFFARLFRRTEQSVEN"
     gene            5865..6083
                     /locus_tag="PLG78_01030"
     CDS             5865..6083
                     /locus_tag="PLG78_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNE21715.1"
                     /translation="MAELTRRLQILLDPDQMESLQAIAQKRRKSVAELIREAVDIQYR
                     PRTEVRRIQAIESLRSFSSIHISTDDLK"
     gene            6110..6508
                     /locus_tag="PLG78_01035"
     CDS             6110..6508
                     /locus_tag="PLG78_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21716.1"
                     /translation="MEKWFLSFDAILVLASDLPAKSALLDQLSEFLKSGILVTSSHSV
                     QRCAEFFEHRGEKSFRSFYSRLRPALDHVLEVTERDQSYALDLAESSNLSLALALEAA
                     VAIHSGASGFVSSNSKIDSLSELPRIAVAI"
     gene            6505..7671
                     /locus_tag="PLG78_01040"
     CDS             6505..7671
                     /locus_tag="PLG78_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE21717.1"
                     /translation="MSQEQVVELTEEESSTQELARRISNQDLAPMADELDRHEQFAHP
                     VFQKLGEAGLLGIVIPEEFGGADGSFLSAVLIMEELARNCGGTALSFGAHAFIGAYNL
                     FKFASDSQRKKYLPDLCSGKKYAAFALTEPENGSDAMGLRTRAEKRGASYILNGAKTF
                     ITNGSVADTFVVFARTGDAGAKGISAFIVERSFPGFASGKPMEKMGMRASPTTELYFE
                     NCEVPAENLLGAEGQGGYFALEGLDMERTLFSGLPIGLIQGAFDVALKYSTERSQFGS
                     KIAGFQLIQAMVADIGMNLFVSRLLAYTAARKLQEKKRVTLNASFSKLFASEASVKST
                     LDCIQMLGGYGYIREFKVERLMRDAKLVEIGGGTSQIQRLIIAREIYKSHDLSP"
     gene            7725..8066
                     /locus_tag="PLG78_01045"
     CDS             7725..8066
                     /locus_tag="PLG78_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21718.1"
                     /translation="MPDLNTDQSRGQVQNLPSASSGPRAIEPVVVPETELPPATGAQR
                     IFWIIIAILCAGWIFIPNFFIIPIPFFIDEGIAAIILMTALSKLGIRIPILHWFFEKR
                     MGKSIALKTKR"
     gene            complement(8067..9374)
                     /locus_tag="PLG78_01050"
     CDS             complement(8067..9374)
                     /locus_tag="PLG78_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE21719.1"
                     /translation="MEDAKLILDNQEYHLPVVQGTDGSRSIHADSLFEKTGLRIFDPR
                     LNNVNITKSAITYIDPVSGRLLYRGYRIEDLVEHSSFVEVSFLLNEGELPNQEQYANY
                     SNELCKHSMIHESMRYFFDAFPGDAHPIAILATMVTALSAYYPSTYEENLKKGVDIRA
                     RLLAKVRTLAAWSYKKSVGEPVVYPRDEMPYCTNFLNMLFAVPAETYQVPKSHDRVLN
                     QLLILYSDHEQNTATTTMRLVASSQANLFACINAAMCAMWGSREFGANIPPIPMLDQM
                     TKRRQSPEEYFEKFIKGQEIIRSNGLGHSSYRTQDPRAIIARKLFHEFRKTNEIAAVD
                     PLFEKALEVEEFTLGHPYFKEKGLYPNLDYYSALIFRMIGLPMTMNNVIRTIGKLSGW
                     LAHWMEQHDEGVRGAYRPGQVYTGVAERPFPTDRQGTHWGRET"
     gene            complement(9429..11402)
                     /locus_tag="PLG78_01055"
     CDS             complement(9429..11402)
                     /locus_tag="PLG78_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE21720.1"
                     /translation="MIRALLVLLFALPLYAQTHDDVLSGDWHVITRDDVVNADPLFDD
                     TSYESVRLPANIADKAKGSGAVWLRKRFNLVRSESNLALLLGAVYDRDRVYLNGELIG
                     EKSSEKYGYGRPRVYPLPASILRQGENTIAIRLEGSFKDFMGITSGPVRIDTIDRAEA
                     TVWKTALKSLVLSGIYIATGIFFIVLFLRVGGMRDYFYYSLFTISFALQQLMRNELRF
                     QIADWFYAFKAAEQMLYIACATSFFFFFVSFFKLKLNRLVYLYPAFNGVVALILPFLG
                     SPVRIDAVMTIWFPLNLPFFAWYAYHACQRALRRDSDAMILAGGMIIMLVATLLFFLS
                     ERGFIKWGGGVFDSSVFIFIFALSFILIFRLIRLHLDVERRRTRLDSVNALRDRVFRY
                     IDGILRGPAQAIVNLTLAARNADLREKEKKELLDKIDTDLGVLQAEMDDILELSRLEV
                     IQEPESFENVNFKDFITAVIPQGAITSYINVDPDIEIKTSLELVNSMVIRLIDFPGFR
                     EFKHIDLIITSDLSENIHFRFLLFHNDFRQTRKLHELITSLNPERGTLWAKWAIVREI
                     IRILDGSMAISIINRKFLRIDIKLKSERVEVQKNVTGEIRIVPIASAIAAEPAMQTAE
                     AVPVPDSMPAPKLSGNMSLGEFAAYIKHKLKRK"
     gene            complement(11399..13252)
                     /locus_tag="PLG78_01060"
     CDS             complement(11399..13252)
                     /locus_tag="PLG78_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21721.1"
                     /translation="MRSWLALAFFCLSHAVYAECDREWVPGNSLDLSGSWKYAQAGEA
                     SSPELDDSGWKRTSVPGLWGKNPALANYRGELWMRCHVTLNGEKPENVALNLGLVEDM
                     DEAYFNGNLVGRTGSFARNTVDIETERLYALPAALWREGDNVIAVRIRGSSTESGIHD
                     QVRIENEQMAARRNFLKNVPAIVCSVLYILTSGFFLLFYVFFRHRYEHMFFALFSAFL
                     GFYHLIRTNLRLDIFTSFEFSYQVELALLFCLPGLFINYLIYLLKLKRPPIVWALMGF
                     SLILVIGALLSGAGKWPIKQAWQILILANLAGLAASMIVILWIVGKNYKERREELRYI
                     VYGFLVLTPAVLHDMLVTLKVYEAPRATVYAFLLFLGFISLQLSDSILDLYRNMKHQE
                     NDLKLLEKKKTTSILNVSSEFKAISEQFEAGVKELRAGKDPESSQLTGASLHLEKFVH
                     DSRLLHMLEEGEYSIRRIRFNLRKLCEDTVQEALSVTGQSKKRLQMEFPDAEILADPD
                     LLSTALYHLIENGLLYTKGRVEAVGEMQGGMVHFAVRDEGPGLNPEMQKQVFLKFERA
                     VDENSDIPGSGIGLAIVSLIAERLGGKVSLEGGGFFSTYRFQFPLSWEGGA"
     gene            complement(13260..14315)
                     /gene="rsgA"
                     /locus_tag="PLG78_01065"
     CDS             complement(13260..14315)
                     /gene="rsgA"
                     /locus_tag="PLG78_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNE21722.1"
                     /translation="MSAESGSFEAVVSSVFGAYYDMLVLPDCLETRRGRLRGRLRLAG
                     RKNEESWDHIPERHLILVGDRVHCVQENDESLIESRRERKNVLFRSSRFEQQGLGANI
                     DRAVVVMSFAYPDVSQGFIDRFLASCHAGGVEPVILFTKSDLTDDELREGCLELYRGL
                     GYSAFAVNLLDPKGEDWESICSLLSNGISLFSGRSGTGKSTLLNRLLGNENMATGAVS
                     ESTKKGRHTTTNSTMVRDPKTGALYIDTPGVKEWGVMNLDRKDIFESFPEIRAAATLC
                     KFRDCLHETGAEGCAVAEKLEKPGEFHPGRILSLKSMLDSLETGERIRKGDYIKATGR
                     MRQGSKYALKNRPPEHR"
     gene            14343..>15671
                     /locus_tag="PLG78_01070"
     CDS             14343..>15671
                     /locus_tag="PLG78_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE21723.1"
                     /translation="MSKVKNTFRQERRRLEDFKRSMIELRATRDVCRAVLEFLTEALD
                     SEKAGIYIWKEEEGTFSVWPDQPGVEPRFSLMDPAIAYFGEHPQVRHVETISSDEDLT
                     DDARELLHRFFTLTNSRVLIPLALNESLVGVIFLAPPPSVKLGRRETALIEEVRSLGV
                     MSLSNSILYARLEGILGHLEEKVRERTQELESTQSQLVQSEKMASLGVMVAGIAHEIN
                     TPASVIQAAAENVEKNISYLLTNLEMLRRVEAKTEESAGSGAADRILHAIESLLKRPD
                     AGPIKDLFLRKRDMTRRMEERAIDHARELSALFAENGLDVDDSLLGDLAAIFQAGKDS
                     GAFLTFLSEAANAARNLKNLKTSIRAIARIVKALKSYSHLDSGAFQLADVHESLDNTI
                     VLLGSVLKTSLHIEKRYAELPDILCNSDELAQVWTNLIQNSAQAMKGNADS"
BASE COUNT         3843 a         3769 c         4050 g         4009 t
ORIGIN      
        1 aaaagagcgt ctgcttggaa tgctcgaaaa actcaaagat cgagtcattg gtcaggatca
       61 tgctctcgta gcagtttctg aagcgattca aagatccaga tctggccttt cggatccgaa
      121 tcgccccatc ggagttttta tgttcctcgg gccaacaggt gttggtaaaa ctgaaacagc
      181 cagagcgctg tctcagtttc tattcgatga tgaaaacgca atggttcgaa tcgatatgtc
      241 tgaatacatg gagaagcaca gcgtggctcg tctcattgga gctcctccag ggtatgtggg
      301 tcatgacgaa ggcggtcagt taacggaagc tgtgcgaaga cgtccatatc aagtgattct
      361 ttttgatgaa gtggaaaaag cgcatccaga agtattcaat gtatttctgc agatctttga
      421 tgatggtcgt cttactgact cgaaaggaag aactgtagat ttcaaaaata caattattgt
      481 tctgacttcc aacattggtt cgcatatttt gatggaaccc ggctcgcaag aagagaaaga
      541 atctcgcatc aaaaaagagc ttcacaattt ctttcgcccg gagtttctca atcgactgga
      601 cgatgtgatt ttctttcagc cagtcggact ggattctctt ctttctattg tgcgaattca
      661 agtggacaag ctcgtaagcc gcgcgaagga acaggctttg aaaatcaaag tggatccggc
      721 tgtgctcgag tggcttgcga agagaggata cgatccgcag tttggtgccc gtccaatgaa
      781 aagattgatt caaaaagacg tgggcaactt attgtcgcga gtgattctca aaggtgaatt
      841 taatacagag aaagagtata cactgagctt gaaggacgat catcctgtcc tcaagggctg
      901 atgcctggga gtgcatatat gcagaagttt attaaaaaga ttcttgttcc agtagatgga
      961 tcggacagtt caaagaaagc tctggagcag gcgatcgctg tggcagaggc tgcgaatgca
     1021 acgatcaccg ttctagaagt agtagaagac ttcggaccgc tgcccggtta ctacgaagcc
     1081 gcgcctaaag gcgaagacag ggtgaaatgg atttcagaac agcgctttga gaaagtgcat
     1141 cctcctctgg acaatacgaa agtgaagtgg gaccgtaaga tcgtggaagg ttatcctgcg
     1201 tatgaaatct gcaaggtggc gcaggaaggg tcttacgatt tgatcatcat aggcagtcgt
     1261 ggtctcaatg cagtcactcg cttcttgatt ggatccgttt ctgatcgcgt cgttcatcat
     1321 gctccctgca ctgttgcagt agttcgctga ccatgcagga ccagcggagc ttcgatgagt
     1381 tcgtcgaggc tctgcttgga tgtcgcaaat gcagcactgt tgtgggtcca cctgtctgcg
     1441 gatccgttcg gagttctaaa atcatagcca ttggtcaggc acctggaccg cacgaagcaa
     1501 gattcgggag gcctttcgcg tacactgcag gccgcactct atttaagtgg atggcctcca
     1561 tcggaatcag tgaagatgtt tatcgcgcga atgtgaatat gagtgcggtt cttcgctgct
     1621 ttccaggaaa agcaaaatct agttccgcca aaagcgcggg agatcgcgtt ccttctcctg
     1681 ccgaaataga aaactgtaag gaacatctgg aatttgaatt tactttccta aaaccgcgac
     1741 tcgtcattcc cattggcaag cttgccattg gtgtgaccat gcctgaggcg gctagatctc
     1801 cgcttgcaga tgtgattggt cgggcgtttc aagtgtctgc attcggcgcg cgatttgact
     1861 gcattccact gccgcatccc tccgggctca atgtctggaa caatgtggag cccggaaagt
     1921 ctctcctggt gaaagctctt tctctaataa aagaacatcc ggctgcgaaa gaattgatca
     1981 aaaaaagctc tgcggcttca tgaatcagtt gcccccatcc tggatgagtg cgaatcatga
     2041 tccatgaaaa aattgatcat ctgtgcagtg cttgcaacgg cattcgcatg cagcaaggca
     2101 cccgaaccgg aagcgccaaa agtagaagcg ccttccaaac gcgtattctt tattgagcca
     2161 gcaaacggcg cgtctgtttc ttctccagtc aaagtaaaga tgggagtaga aggaatgacc
     2221 gtagagcccg caggcaagat cgtagaaggc acggggcact ttcacatcat cattgatgca
     2281 ccagcttcag aagaagggaa ggtagttccg gcggatgatt cgcacaaaca ctatggcaaa
     2341 ggccagaagg aagcggatct gactctcaag ccgggcaaac acaaactggt tcttcagttt
     2401 gcagacggtg agcacaaatc ctatggcgaa aaattgacgg acaccatcga gatcacagtg
     2461 aagtagttca cattcttctc agatgggaag gcggcttttg gccgcctttt tgtttttaag
     2521 gatgcacttt ttgcataaag tgagcgagtt cggccaggtt gtgcgccaca tcgtaggtct
     2581 ggatttgaac gcccagctgt cgcgcaatct gagtcagtga gaagataccg cgcagagtga
     2641 tttcattgcg tgggccctgg ttatccacca cgattccatg ctgtctgtgg tgatgcttga
     2701 gtgtttcgat gatgtctccg actctcgcat tgctcacatc tttgatgctc agagcgtcta
     2761 tgtctgactg gcccgtcatg atatcggata cttttagatc tcttgcctgt ccgccgttct
     2821 tttgaacaaa ctggattggc ttttcgccca gagtatccga agcggtgatg attcccacga
     2881 gctcatcgtt gtagtcaaca accagaagca tgcgaacacc gcgatgaatc atcttctggt
     2941 gagctgcatc cagcgattcc tgtggatgaa tggtgatggc tgttgtcttt tttaagtcag
     3001 tcatcacctc cagcgccgga gaacttacag ttacaggcgc gtgcctgtga tgatcgggcg
     3061 tgtgaaatgt agttccttta gaaacagtgg aagaagtcag tacgcgaaag ctcatcgtag
     3121 gtccacctga acgacgctca aaatattctc aagtcttttc atcttctcga gggcatctgc
     3181 ggacagcaat tcgtctgttg taatcacgca tctcgcggtg ccccctcttg aatcgcggcc
     3241 aagttcgagg tgagcaatat ttacgttttg atctcccaga tacatgccca gagtgcccac
     3301 tacgcgcggt acatctctgt tttgcacgat caaaaggatg ccttccggtt caaattcgat
     3361 gggcaggttt tgaaaaccta ctaccattgg cttactctgc agtgccgtga atttcagacc
     3421 ggccgttgtc ttgctggatg aaaatttcac ggtgatcgcg ctggaataac cttcgcatgt
     3481 cttttcgctg gtttcagtga ctcgaatgcc gcggtctttt gccaggaaag gtgcgtttac
     3541 aaaattcact tcatctccga gcgcaggcgt gagtgctccg cgaagaatgg cagccttcac
     3601 aggatcggtc atgaattttt caatttcgcc tgcgtattcg atctcgatgg aatttacatc
     3661 cccctcaagg aattgagtga gaaggcgacc aattttttct ccgcctttga aatatggtgc
     3721 cagaaaatcc agcgtctctg gttccatggt ggggaagttc aaagcgtatc gtgcaattcc
     3781 ttttgtgaag aaatcaatca cctgttctac tgtgtccatg gcgactgcaa actcggcatc
     3841 tcctgtggac gctccgaggt gaggtgttac tatgcagttc tcaagcttca agagaggatg
     3901 gtctgcagca acaggctcct gtacaaacac atcgagtgcg gcggccgcaa tcgttccatc
     3961 tgaaaggccc tgagcgagcg catcttcgtt gtaaatcccg ccgcgcgcgc aattgatgag
     4021 acggattcct ttcttgatct gagaaaggtt atctttactt acgaaatcct ttgttgtttc
     4081 tgtaagaggt gtgtgcactg ttacaaagtc tgattgtttg agtatctctt cgcgagatac
     4141 aatgtcaatt tccaggttgg ccagatttgc aggtggaatg aaaggatcat agccaatgac
     4201 tctcatgcga agtcccttcg cgcgctttac aacttctttg ccaattctgc caagccctac
     4261 aactccgaga gtttttccag tcagctctac tccgaggaat ttgctttttt cccatttgcc
     4321 gccgcgagtg gatgcatttg cctgtgggac gtttctcgcc atgctcatca tcaaactgat
     4381 ggcatgttcc gctgtggaaa ctgagttacc tccgggagaa ttcatcacaa ccacaccgtg
     4441 acgcgaagct tctggaatat caatgttgtc tacgcctaca ccggcgcgaa ctacgagctt
     4501 caaattcttt gccgcggcca gcacttctgc gtctacagtg gtggcagatc gaatgatgag
     4561 gccgtgcaca tttcctatga tcttgagtag ttcgtcgcgt tttgttccgc cggagtaaat
     4621 tacttctaaa tccgcatggg ctttgagttt ggagacgcct tctgcgtcga atttatcagc
     4681 aatgagtatg gtatgcttca tatgagccat gttttaggag ggcacccttc gctaaaagtg
     4741 atttagcgga gcgaaagccg cagttttcgc acggcccaga gacggtcaat tttcgacgga
     4801 ctgctctgtt ctcctgaaaa ggcgagcaaa gaagccccgc cggggcgtct ccgggacaaa
     4861 gtctttgaga gtgtcgtcga cgaattcctg gatgaaacgc agtactctct tctgcgcttt
     4921 tttgtgggtc aaaagagtat gcatgtttgt catatcagga ggcagaatga actggagagc
     4981 ccgtttttct ctgcttccaa tgttgttgta gatgtagatt tgattttccg cagacacggt
     5041 tcgatcgttt gcagccatga tggagcaata aggaacgctg atgcgaggca gaactcttct
     5101 cactccggag agagatctct tgaaactatg gagagtatcg agcgcgtagg tggcgtcata
     5161 tccaagccag gggttcagtc tttgcatgct attggttttc tttttccctt ctatgcgaag
     5221 aatagaagta aagatgcgca tgattccagt aaacatggtc atgggctggt ggatgatgag
     5281 ttttccgttg aaaaacccat tgaagaaaac gggagatgaa atggtcagca ctccagcggg
     5341 gcgatacgta tctgcgaatc tgcccgcaag atcgagcatc agtgtgccac ccatagaaaa
     5401 accaaccagg aatacttttc tgtatttgct tctctcttct ctgaatagag tctccagata
     5461 gtcttgccag tgatagaatc ttacgcgcgt tagatcttcg atttttgtcc cgtggcctgg
     5521 aagaagaatg ccgatcgaag tatgtccgag tccatgcagg aattctccca gcggtcgagt
     5581 ggtcagaggt gaatcttcaa atccgtggca gatgagaaaa gccactccgc ttgtgccttc
     5641 gaatctgtac ggttccgctc ctggcatgat ctgcctcggg tcgattggct caggcatggg
     5701 ccgggccggt tcaggcaatc caaagtggcc aagaatgaat atagaagcga cgagaagaac
     5761 gaggatcgac aggaccacgt cgcgatgttc gccgagtcgc taattccggc aaatgaaatt
     5821 cccggcacga gttgacgcag agcacgggga tgcagtcatg agtcatggcg gaactcacgc
     5881 gcagactgca aatcctgctc gatccggatc aaatggagtc attgcaggca atcgcgcaaa
     5941 agcggcgtaa gtctgtggcg gaactcatcc gcgaagcggt cgacattcag tacagacctc
     6001 gcactgaagt aagacgcatt caggccatag aaagtcttag atcgttctct tcgatccaca
     6061 tttccacgga cgatttgaag taatctgaaa attttgaagt agtctgaaaa tggaaaagtg
     6121 gttcctcagt tttgatgcca tcctggttct cgcctccgat ctacccgcga agtccgcgct
     6181 tctcgatcaa ctgagcgaat ttctaaaatc aggcattctg gttacatcct cgcactcagt
     6241 gcaaagatgc gccgagtttt tcgaacacag aggagagaaa tcgtttcgat ccttctattc
     6301 ccgtctgagg cccgctctgg atcatgttct ggaagtgacg gaaagagatc agtcttacgc
     6361 tctggatctg gccgaatctt ctaacttgtc tctggcgctg gctcttgaag cggctgtggc
     6421 gattcattca ggcgccagtg gcttcgtatc atcgaattca aaaattgact cgctttcgga
     6481 acttccgcgc atcgctgttg ccatatgagc caggaacagg tagtagaact gacagaagaa
     6541 gagtcttcca cgcaggagct cgcgcgtcgc atttcaaatc aggacctggc tcccatggca
     6601 gatgagctcg atcgccatga gcagttcgcg cacccggtgt ttcagaaact cggagaagct
     6661 ggattactcg gaattgtcat tccggaggag tttggagggg cagatggatc gtttttgtcc
     6721 gcagttctta ttatggaaga acttgcgcga aattgcggcg ggactgctct gagttttgga
     6781 gcgcacgcat tcattggcgc ttacaacctt ttcaaattcg caagtgattc gcagcgaaag
     6841 aaatatctgc ctgatttgtg ttctggtaag aagtacgccg cctttgctct cactgagccg
     6901 gagaacggat cggatgcgat gggtcttcgc accagggcag agaagcgtgg agcatcttac
     6961 attctcaatg gcgccaaaac tttcatcaca aacggaagcg tcgcagacac atttgtagtc
     7021 ttcgcgcgga cgggagatgc aggcgccaaa ggaatttccg cattcattgt ggagagatct
     7081 tttccgggat ttgcatcggg taaacccatg gaaaagatgg gaatgagagc gagtccaact
     7141 acggaattgt atttcgaaaa ctgcgaagtg cccgcggaga atttgctcgg tgccgagggg
     7201 cagggcggtt actttgccct ggaaggtctg gacatggagc gcactctttt ttcaggcctg
     7261 ccgattggac tgatccaggg agcattcgac gttgcgctca agtacagcac ggaaagatct
     7321 cagttcggtt caaagatcgc tggctttcag ctgattcagg ccatggtggc agacattgga
     7381 atgaatttgt tcgtgtccag gcttcttgcc tacacagcag ccagaaagct ccaggaaaaa
     7441 aagagagtca ctctcaacgc aagtttctcc aaactcttcg catcggaagc aagtgtgaaa
     7501 agcactctcg actgcatcca gatgctgggc ggttacggct acattcgtga attcaaagta
     7561 gaaaggctga tgagagatgc aaagcttgtg gagataggcg gaggcacttc tcagatccag
     7621 cgtcttatca tcgctcgcga aatctacaaa tcgcacgatt tgagccctta gaacattttt
     7681 tgtttttact tgatctctgg cgcagtcggg tcgtctaatc gattatgcca gatcttaaca
     7741 ccgaccaatc ccgcggacag gtgcagaacc ttccatccgc atcgagcggg ccacgcgcca
     7801 ttgaaccggt tgttgttcca gaaacagaac ttccgcctgc cacaggcgca caaagaatct
     7861 tctggatcat cattgcgatt ttgtgcgcag ggtggatctt cattccaaat ttcttcatca
     7921 ttccgattcc ttttttcata gatgaaggaa tcgcggcgat cattctgatg accgcactct
     7981 cgaagcttgg aatccgcatc cccattctcc actggttctt cgagaagaga atgggaaaga
     8041 gcattgctct gaagactaag cgctgatcag gtttcccgac cccagtgtgt cccctggcga
     8101 tctgttggaa atggtctttc cgcaacgcca gtgtacacct gtccgggtct gtatgctcca
     8161 cgcactcctt cgtcgtgctg ttccatccag tgcgccagcc agcctgaaag ttttccaatc
     8221 gtgcgaatca cgttgttcat agtcatgggc aggccgatca ttcgaaagat gagcgcggag
     8281 tagtaatcca ggttcggata cagacccttc tctttgaaat agggatggcc cagggtgaat
     8341 tcttctactt ccagagcttt ttcgaagaga ggatctacgg ccgcaatttc gtttgtcttc
     8401 ctgaattcgt gaaagagttt cctggcaatg atcgcacgag gatcctgagt cctgtaactc
     8461 gaatgaccga ggccgttgga acggatgatt tcctgccctt ttataaactt ttcaaagtat
     8521 tcttccgggc tctggcgacg ttttgtcatc tggtcgagca tggggatagg cggaatgttt
     8581 gctccgaatt ccctggatcc ccacatcgca cacatcgcgg cgttgatgca ggcaaacaga
     8641 tttgcctgtg agcttgccac gaggcgcatc gtggttgtag cagtgttctg ttcatgatcg
     8701 ctatagagaa tgagcaactg attgagcact cgatcgtgtg atttaggaac ttgatatgtt
     8761 tctgccggaa cagcaaagag catgttcaag aagtttgtgc aatacggcat ttcgtcgcgc
     8821 gggtatacta ctggctctcc tacagatttc ttgtaagacc aggcagccag tgttcgcact
     8881 ttggcaagaa gcctcgcgcg aatatctact ccctttttca aattctcttc atacgtagac
     8941 ggatagtatg cggacagagc agttaccatg gtggccagga tggcgattgg atgggcgtct
     9001 cctggaaaag catcgaagaa gtagcgcatg gattcgtgaa tcatggaatg cttgcacaac
     9061 tcgtttgaat agtttgcgta ctgttcttga tttgggagtt ctccttcatt gagcagaaag
     9121 gaaacttcta cgaagctgga gtgctctacc aggtcctcaa tgcgatagcc acgatagagt
     9181 agccttccgc tcactggatc aatgtatgtg atggcgctct tcgtgatgtt gacgttgttc
     9241 aagcgcggat caaaaatgcg aagccccgtc ttttcaaaga ggctgtctgc gtgaattgaa
     9301 cgcgagccat ctgtaccctg cacgacgggc aggtggtatt cttgattgtc tagaatgagt
     9361 tttgcgtctt ccatagcctg gcatgattcg gcgccaggcg ggcatggttg caagcactat
     9421 ctctcgggtt atttccgctt cagtttgtgc ttgatgtagg ctgcaaattc acccagagac
     9481 atgttcccgg atagctttgg cgcgggcata gaatctggca ccgggactgc ctctgcagtc
     9541 tgcatggccg gctcagctgc aatggcagat gcaatcggca caattcgaat ttcacccgta
     9601 acattcttct gcacttccac tctctccgat ttcagtttga tatcaatgcg caagaacttg
     9661 cgattgatga tcgagatggc catggagcca tccagaattc gaatgatttc gcgaacgatc
     9721 gcccatttgg cccagagagt gcctcgttcc ggattcaaac tcgtgatgag ttcgtgaagc
     9781 ttgcgcgtct ggcgaaagtc gttatggaaa agaagaaagc gaaagtgaat gttttctgaa
     9841 aggtcagatg tgatgatcag gtcaatgtgc ttgaactcgc gaaaccccgg gaagtcgatc
     9901 aggcgaatga ccatcgagtt gaccagttcc agggatgttt tgatttcaat gtcaggatcc
     9961 acgttgatgt aggaagtgat cgctccctgc ggaatcaccg ctgtgataaa gtccttgaaa
    10021 tttacatttt caaaagattc cggctcctgg atcacttcga gtctcgatag ttccaggatg
    10081 tcgtccatct ccgcctgcag cacgcccagg tctgtgtcga ttttatccag cagttccttt
    10141 ttttctttct ctctgaggtc cgcgtttcgc gcggcaagag tcaggttgac gattgcctga
    10201 gcgggcccgc gaagaatccc gtcaatgtat ctgaacacgc ggtctcgcag cgcgtttaca
    10261 gaatccagac gagtacgccg tctctccacg tccaggtgaa gccgaatcaa acggaaaatc
    10321 aggatgaaag aaagcgcgaa aatgaaaatg aatacgctcg aatcaaagac tcctccgccc
    10381 cacttgatga atccacgttc gctgaggaag aatagaagtg tggccacgag catgatgatc
    10441 atgccaccgg cgagaatcat tgcatctgag tcccggcgaa gtgctcgctg gcatgcatga
    10501 tacgcatacc atgcaaaaaa gggaaggttc agcgggaacc agatggtcat cactgcgtcg
    10561 attcttacag gcgagccaag gaatggcagg atcagagcca caactccgtt gaaagccgga
    10621 tagaggtaaa ccaggcgatt gagcttgagt ttaaaaaagc taacaaagaa aaagaagaag
    10681 gaagtcgcgc atgcaatgta gagcatttgc tctgctgcct tgaatgcata aaaccagtct
    10741 gcgatctgga aacgcagttc gtttctcatc agttgctgga gagcaaagga aatagtaaat
    10801 aaactatagt aaaaatagtc tcgcatcccg cccacgcgga ggaatagcac tatgaagaag
    10861 atacctgtgg caatgtagat tccggaaaga accaggcttt tgagggcagt tttccagaca
    10921 gttgcttccg ctcgatcaat cgtgtcgatt cgcactggcc cggaagtgat tcccatgaag
    10981 tctttgaaag aaccttccag gcgaattgca attgtgtttt cgccctgacg aagaatagaa
    11041 gcggggagtg ggtacactcg cggtctgcca tacccgtatt tctcggatga tttttctcca
    11101 atgagttccc cattcaaata cacgcgatcg cgatcataga ctgctccaag gaggagcgcg
    11161 agatttgatt cgcttcgcac aaggttgaat cgttttctga gccagaccgc tccacttcct
    11221 tttgctttgt ccgcgatgtt tgcggggagg cgcacagact cataggaagt gtcatcgaag
    11281 agtgggtctg catttactac atcgtccctt gtgattacat gccagtctcc agaaagcacg
    11341 tcgtcatgcg tctgtgcata gagaggcagt gcgaaaagaa gaaccagtag agcgcgaatc
    11401 atgctccgcc ctcccagctc agcgggaact gaaagcgata cgtagaaaaa aatccgccgc
    11461 cttccaggga aactttgcca cccagtctct ctgcaatgag acttacgatc gccagaccaa
    11521 ttccggagcc tggaatgtcg ctgttctcat ctactgcgcg ttcaaacttt agaaagacct
    11581 gcttttgcat ttctggattg agtcctggcc cttcatcgcg cactgcaaaa tgcaccattc
    11641 caccctgcat ttcgccgact gcttcgactc gcccttttgt atacagaaga ccgttttcta
    11701 tgaggtgata gagtgcagtg ctgagtagat ctggatctgc gagtatttca gcatctggaa
    11761 attccatctg cagtctcttt ttggactgac cggtcacaga caacgcttcc tggacagtgt
    11821 cttcgcagag cttgcgaaga ttgaaacgaa ttctgcggat ggagtattcc ccctcttcga
    11881 gcatatggag caatcgcgag tcatgaacga atttttccag atgaagagat gcgcccgtta
    11941 gctggctgga ctctggatct tttcctgcgc gaagttcctt aacgcccgct tcgaattgct
    12001 cagaaatcgc cttgaattcg gaagaaacat tgagaataga agttgtcttc tttttctcca
    12061 gaagtttcaa atcgttttcc tgatgcttca tgttgcgata aaggtccagg atggaatcag
    12121 aaagctgcag cgaaatgaat ccaaggaaca atagaaatgc gtagaccgtg gcgcgcgggg
    12181 cctcatatac cttgagagtg accagcatat cgtgcagaac cgcgggtgtc agaaccagaa
    12241 atccatacac aatgtaacgc agctcttctc ttctctcttt gtaatttttc ccaacgatcc
    12301 agaggattac aatcatggag gcagcaaggc cggcgaggtt tgcaagaatc agaatttgcc
    12361 acgcctgttt gataggccac ttgcctgcac cggacaagag cgctccaatc acgaggatca
    12421 gactgaaccc catgagagcc catacgatcg gtggacgctt gagcttcagc aaatagatga
    12481 gatagttgat gaacagtcct ggaaggcaga agaggagggc cagttctacc tgataggaga
    12541 actcaaacga tgtgaaaatg tcgagcctca ggttggttct gatcagatga tagaatccta
    12601 gaaaggcaga gaacaatgcg aagaacatat gttcgtatct gtgccggaag aaaacgtaga
    12661 agagcaggaa gaagccagaa gtaagaatgt aaagcacaga gcaaacgatc gccggaacat
    12721 ttttcaggaa gtttcttcgc gcagccatct gctcgttttc aattcgcacc tggtcatgga
    12781 tgccggattc agtagagctt ccgcgaatgc gaactgcaat tacattgtct ccctcacgcc
    12841 agagcgctgc tggaagtgca tagagtcttt ctgtttcgat gtctaccgtg tttcgcgcga
    12901 atgagccagt ccttcctacc aggtttccat tgaagtaggc ttcgtccatg tcctctacca
    12961 gacccaggtt gagtgctacg ttttctggtt tttcgccgtt gagtgtgacg tggcagcgca
    13021 tccacaattc gccgcggtag ttcgccagtg cgggattctt tccccacagt ccagggacac
    13081 tcgtgcgctt ccagcctgag tcgtctagtt caggcgaaga cgcttctccc gcctgggcat
    13141 acttccagct gccgctcagg tccagtgaat tgccgggcac ccattcccga tcgcactctg
    13201 cgtagactgc gtgggaaaga caaaagaatg ccagggctag ccagctccgc atagattcat
    13261 catcggtgct caggggggcg attcttcaag gcatacttgc ttccctgtcg cattcttccc
    13321 gttgctttta tgtagtctcc cttacggatt ctttccccgg tttccaggga atcgagcatg
    13381 gatttcaggc tcaggattct gccagggtga aattcaccgg gtttttcgag cttttctgcc
    13441 acggcacaac cttctgcgcc agtttcgtgg aggcagtctc tgaatttaca tagagtggcg
    13501 gccgcgcgaa tttcaggaaa actttcgaag atgtcctttc tgtccaggtt cattacgccc
    13561 cattccttca cacccggagt gtcaatgtag agagcgcccg tttttggatc tcgcaccatg
    13621 gtggagttgg tagttgtgtg cctgcctttc tttgtggatt ctgataccgc gcccgtggcc
    13681 atgttttcgt ttccaagaag gcgattgaga agagtcgact tgcccgtgcc tgatctaccg
    13741 gagaaaagtg aaattccatt ggaaaggaga gagcaaatag actcccagtc ttctcccttt
    13801 ggatcgagca agttcactgc gaaggcggag tagccaagac cgcggtagag ctccaggcat
    13861 ccttcgcgaa gttcatcgtc tgtaagatct gattttgtaa agagtatgac aggttctact
    13921 ccaccggcat ggcagctggc gaggaatcga tcaatgaacc cctgggacac atccgggtag
    13981 gcgaagctca tcactacaac tgcgcggtca atgtttgcgc ccaggccctg ttgctcgaat
    14041 ctagacgatc gaaacagtac gttttttctt tcgcgtctgc tttctatgag gctttcgtca
    14101 ttttcctgca cgcagtgaac acgatcgccc acgaggatca ggtggcgttc tggaatgtga
    14161 tcccaggact cttcgttttt gcgccctgcc aggcgaagcc gtccgcgaag tctgcccctc
    14221 ctggtttcga ggcaatcagg gagtacgagc atgtcatagt aagccccgaa tacactggaa
    14281 acaactgcct caaagcttcc ggattcggcg ctcacaggtc cgatatacta agagcacgcg
    14341 gcatgagcaa agtaaaaaac acattcaggc aggaaaggcg caggcttgag gattttaaac
    14401 gatccatgat cgagcttcgt gcgacccgtg atgtatgccg ggccgtgctc gaatttctaa
    14461 ccgaggcgct cgattccgag aaagcgggca tctacatctg gaaagaggaa gagggaacat
    14521 tttctgtgtg gccagatcag ccaggtgtgg agcccagatt ttctttgatg gatcctgcga
    14581 tcgcttattt cggagaacat ccccaggttc gccacgtaga gaccatttcc tccgatgagg
    14641 atcttacaga cgatgctcgt gaattgttgc atcgcttttt tacacttacg aattcaagag
    14701 tgctcatccc ccttgctctc aatgaatctc tggttggagt catttttctt gcaccacctc
    14761 catccgtgaa gcttggccgc cgggagacag ctctcataga agaagtcaga agcctcggag
    14821 tgatgtctct ttccaattcg attctgtatg ccaggctcga agggattctg ggtcatctag
    14881 aagagaaagt acgcgaaaga acccaggagt tggagagcac gcaatcacag ctggtccaat
    14941 ctgagaagat ggcatctctc ggggtgatgg tggcgggcat tgcacatgag atcaacacgc
    15001 ctgcatctgt catccaggca gcggctgaaa acgtagagaa gaatatttcg tatttactta
    15061 cgaatctgga gatgctcagg cgagtggaag caaagacgga agaaagtgcc gggtcgggtg
    15121 cggcagacag aattcttcac gccatcgaat cccttctcaa aaggccggac gcaggcccaa
    15181 tcaaagatct gtttttgcga aagcgcgaca tgaccagaag aatggaggaa cgagccattg
    15241 accacgcacg tgagctcagc gctttatttg ctgagaacgg actggacgtg gacgactctc
    15301 ttttgggtga tctggctgcc atctttcagg caggaaaaga ttctggagcg ttcctgacgt
    15361 ttctttctga agcggccaac gctgcgcgca acttgaagaa tctgaaaact tccatacgtg
    15421 cgattgcgcg cattgtaaaa gcactcaagt cttattctca tcttgattct ggagcattcc
    15481 agctcgcaga cgttcatgaa agtctggata ataccattgt gcttctggga agtgtgctca
    15541 agacttcttt gcatattgag aaacgttatg cggaattgcc ggacattttg tgcaacagtg
    15601 acgagctagc acaggtgtgg accaacctca ttcagaattc agcgcaggca atgaaaggaa
    15661 atgcggactc t
//
LOCUS       DAOQJL010000013        15649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1787_length_15649_cov_4.962614, whole genome shotgun sequence.
ACCESSION   DAOQJL010000013 DAOQJL010000000
VERSION     DAOQJL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1787_length_15649_cov_4.962614"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLG78_01075"
     CDS             complement(<1..344)
                     /locus_tag="PLG78_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21724.1"
                     /translation="MKRCRLTLLLIALLMVASCKGKNAEPLLSSLLGMNSKTVLILKG
                     TYASDSPLEFTELNNNNLYVDAEDTSVDLVGVPRYSQLPIYLDIGEIRLSSKDYGNGL
                     WSIDTAKDAKAF"
     gene            complement(352..990)
                     /locus_tag="PLG78_01080"
     CDS             complement(352..990)
                     /locus_tag="PLG78_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21725.1"
                     /translation="MRKSRILICIVLLAAGTAASSLHAQEQQSASRVKHEIGLWIGAS
                     NPLPGTELDAALDSNLGGGLFYRMTWPWILYTEFGTSYSNYFSRETQSATVIPIYAAL
                     SYPIQLPIRMQVFLKAGGGTAYVLVRPINRHGWEPLGYLGTEFSILAGRRFRIGLRID
                     YNLVYEKHLKDPEQPLLPITTGTTDPRYQKTQYFKKRNGHFFHFGLMTSFIL"
     gene            complement(977..1828)
                     /gene="truA"
                     /locus_tag="PLG78_01085"
     CDS             complement(977..1828)
                     /gene="truA"
                     /locus_tag="PLG78_01085"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNE21726.1"
                     /translation="MVSYDGTKYAGFQYQKSGTSIQELLENALQIVVREHTRVDCAGR
                     TDSGVHATGQVATFLSPVAPDPGKLTYSLNSLLPFDVSVQKSLNMPRNFHPRFSCIAR
                     EYEYMYHIGARSPFWRGRSWHRKKGFEIEEINQELAAICGHRDFIALTKTLYEGKGTV
                     RYLDSAVLYRKEDPWTGHGDMIVLRVRANAFLHNMIRILAGTIVDRAAGKLSSITDAV
                     LSGSRFKTGQTAPSEGLYFRHAFYVPGTAPGLKELALPDDHPLMRIHAGRMRSSREGD
                     QENHAKE"
     gene            complement(1848..2756)
                     /locus_tag="PLG78_01090"
     CDS             complement(1848..2756)
                     /locus_tag="PLG78_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNE21727.1"
                     /translation="MRIKQPEISKAKAISYRVKDKSTCKVCGFEFNREQLHSGGGRLI
                     AGKLTQELRRLYEVSKRYGKVYPLAYSILTCPQCLFSSFPTDFALLPPDEVEKLKKAT
                     LDRRTSVEKIVGPVDFADDRNLVSGAASYLLAIDCYQMRGMGIAPTPKKAICALRGAW
                     LFQDMAEEFPKYGFEKLSEFLYLKSVSWYIPTLEIMTNGREPHEQFINLLGPDTDNNW
                     SFDGVIYINGYLTRKYLNQLAPTKEKKLEYLDRSKRYLGKLYGMGRASKSKPSVIIDM
                     AKELYEELQKEIDTINGVPPTAPAAS"
     gene            2873..4021
                     /locus_tag="PLG78_01095"
     CDS             2873..4021
                     /locus_tag="PLG78_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21728.1"
                     /translation="MKRRLAFLLLLCLPVAIHAESVAEKATEKRNELIRYLREIRTMA
                     YNYPCDPFPDCQAALPQGDDKKAPATPAGTTAQPERIKLFGDIKRIYQEGMVYLYEGN
                     FVNSYNRFLDSQARVERMLEGLSQLYLDRAEAMMRDSIEKKDPTDPADMTATDISIDY
                     GQKSDKRRDFARNREIPRDSRTYDQKEVHYAHNKYEIERNIEMGYKHLGLAREARRRA
                     LIVDTNLAPNQRMEPHHRRARIEYYLDCIRMARLAKLNGERIYQLKYPYDNYALISPF
                     GKTEIVRGDHPKEAKFPVIAGKERDWSTDENTADANKKADKSNPYIYPANLNPVFDLR
                     IPEKYRRDSSDARDQIYADQIDINVDFRYIPEKRNKPTADSTTPAKQP"
     gene            complement(4097..4996)
                     /locus_tag="PLG78_01100"
     CDS             complement(4097..4996)
                     /locus_tag="PLG78_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21729.1"
                     /translation="MEIVRRIRTLNEEGKRTSIPFSDTIYRKLLSDLCESEDRMRFYL
                     RVLSDAHYLFTIRLVEPDERLLIHGLEAYVVCDVPVIMRLKEVSYSELELSYEGQYYK
                     RKQATLILRELIGNARQFNNTPLGKSMNLAMMLQQYEQFMATRFAEFSEGWKQKKLNE
                     LLPEVGFDIGSGSATASSAKTEAQPRRAIDSEHLQKIEEMNRSGKWGEAVDKFGVQFL
                     VRIHLRKYEFEQIKTLIKQHKIATEKDLRFLRDTLKMMEGRFEEDPELVKHKEEMIEL
                     RRLAQLKMNQVLIARKEFESQGN"
     gene            complement(5014..5754)
                     /gene="kdsB"
                     /locus_tag="PLG78_01105"
     CDS             complement(5014..5754)
                     /gene="kdsB"
                     /locus_tag="PLG78_01105"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNE21730.1"
                     /translation="MKVLGVIPARYGSQRFPGKVIAPLGKKPMVQWVYEAASRCKDLD
                     HLVIATDDERVRDAVKAFGGEVLMTSEDHKAGSDRLAEVSESYKDYQIVVNIQGDEPG
                     IEPDLISGVVQLLKDHPEWEVTTAARPFEDAEDPLIPNRVKVTLTKKGKVLYFSRSLI
                     PFPRNKTGHPVFLHLGIYAYRRDFLLRFRTLPGSGLEDTESLEQLRVLENDYSMGAHI
                     VKGSLPGVDTPEDLQNITAIFRKKGMLE"
     gene            complement(5792..6481)
                     /gene="queC"
                     /locus_tag="PLG78_01110"
     CDS             complement(5792..6481)
                     /gene="queC"
                     /locus_tag="PLG78_01110"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNE21731.1"
                     /translation="MQDVSTKAEGAVVLLSGGLDSATVLQIAKSHGYSLIALSFDYGQ
                     RHKFELERARELAIRANAKHLVLRIDQELFKNTALVPGGPAVPEKRQIDDSIPVTYVP
                     ARNILFLSHALAVCESNDIGNIFIGVNALDYSGYPDCRPEFIQAFEQMAALGMKRGVE
                     GRPIIIHAPLMQWNKARIIQEGMKLGVDYSRTSSCYQPSASGKPCGLCDSCVLREKGF
                     AEAGLIDPLKS"
     gene            complement(6456..6860)
                     /gene="queD"
                     /locus_tag="PLG78_01115"
     CDS             complement(6456..6860)
                     /gene="queD"
                     /locus_tag="PLG78_01115"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNE21732.1"
                     /translation="MQQEFDSLHKPAESRVVEIVKQFRFEAAHFLPRVPEGHKCGRMH
                     GHSFRFEIKLNGEVNPQTGWLMDFGDITSVVKPLLEKYLDHNLLNEVPGLENPTSEEV
                     AIWLWKRLKPSMPFLTEIIVHETCNARCIYKG"
     gene            complement(6844..7497)
                     /locus_tag="PLG78_01120"
     CDS             complement(6844..7497)
                     /locus_tag="PLG78_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE21733.1"
                     /translation="MSQDKNIRIIETYLSVQGEGMHAGLPCFFIRTAVCDIRCSWCDT
                     PQALGNGPSISFEDLLARVPTSVRLIQITGGEPLVQKDQVIALCEALHARGKKILLET
                     GGHRSLDGIPDYVHIVMDIKLPGSGQAHHPFEKNFEWLKKSDEIKFVIQDRIDFDAAL
                     SWIRSHNLENVCHLLLSPVWGSVAPATLTQWLLEENIDARMQIQLHKIIWGANATGV"
     gene            7541..8743
                     /locus_tag="PLG78_01125"
     CDS             7541..8743
                     /locus_tag="PLG78_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21734.1"
                     /translation="MLVVRFAQIVFLAGLVAGIVFLFWSWRRGVRFIQIERARLRRVD
                     TVEWNPSIPRDFLNEVLSPDRIELCSIAQDASLVRICQWMAEQSLSVAAIPARGPSGS
                     GVCILRPGALQDGFVWRLFFDRNLPRPSTLEAVVARMDLFVRSGEKAESPASAPGETA
                     EYRLSQLRALELSVQRIAHDIRIPVASLSLIGENLGVEWESIRRTEASVHMDGLLIRL
                     RRQIANLELFARGFLDLEKGAQPASEVKTLDVGALWLSLLDAHAEEMKKKSLRLKIAG
                     DMTGQMVRCAESDLVRILENLLSNALKFSLPGGCIWTSVRGEESDIVLDLEDCGPGFT
                     ASTEMDPFDLARRNLGRTGTGYGLGLAAALDLAESMNGKLSLANPLHGAGARFLLVLP
                     GAELSLDT"
     gene            8740..8967
                     /locus_tag="PLG78_01130"
     CDS             8740..8967
                     /locus_tag="PLG78_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21735.1"
                     /translation="MTGIHLRNTTGKRSGSFIRYEYGEDLFGYVYLDVIRGRRHRAQK
                     VWTALFTSKIDFLITLDMDLERKENLNYVHA"
     gene            9032..11440
                     /locus_tag="PLG78_01135"
     CDS             9032..11440
                     /locus_tag="PLG78_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE21736.1"
                     /translation="MENSVFVLGSGPLGLILSSNLSEKASSVTLCWHDSAEAARMRRD
                     GGTRLLNQDFFFPDNVEIADRTAYLPDDCILIVAISSRELEETMEAMLASFKDRSGLI
                     VLFTKGLPSYTARKKYNARTFCEYMEKFSHDRGIHQLKFAAVNGPSLLSELYHGSYSF
                     LNVGCADTDAGKRIAELLSNDRIQCGYTSDTIGVEMGGVLKNPIAIASGIATNLAECG
                     GNFAGELIQHGFQEMLSLSLAIGARQETLIGRSGIADLMTTCLSKESRNRAYGEDFIK
                     RLQSGSEEHGVMDRIETFLNPARVIQKDAAQGQDVVEGAYAIASIVEIAAELKVEVPL
                     YNALYEILSRRSPPNALISAITGAPVRASEPQVLHRRQGLHLAAGTDFVEVLSSRVFH
                     SVQVTKGMQSRIKKQATQVLTQLDKRLQKAKKVKNRMDLQKIPHERDLWLSFSSSIGE
                     SESRALRHLIQFYAEEIADSYTPGMRETLIRLLAPVRFAASGFKLGSAMPKIGGNTEE
                     LKNLAAHYNVMYTPTHRSHLDSVEVAFGLSWLSVPLPRYAAGINLMADQFQSWLLRSF
                     GAYAVDRERTRNFLYLECLSSYACLMLESGIPSLVYPEGTRSRDGKIVGIKTGLLSTA
                     VSAYQNTGREVVVMPLALSYESIPEDSFYCGLGEEPGLKEFINKRGSAYLDICDPIRV
                     SRHMSADDPTLSISMAIVEQWKKHLRILPNQILARILSDNGHAVPRAGLPALVEEFVL
                     RNPGNYLTRDAEAIVEQGIASLRERNIAKEDGVQLASVTPALASYYGGMVPESGGTIL
                     DQDA"
     gene            11549..11710
                     /locus_tag="PLG78_01140"
     CDS             11549..11710
                     /locus_tag="PLG78_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21737.1"
                     /translation="MSWLWPGPRSDNFKNLHKTQSYKASDFTCAAFEDAELLAKAKLL
                     EQEAAEEKQ"
     gene            complement(11719..13002)
                     /gene="xseA"
                     /locus_tag="PLG78_01145"
     CDS             complement(11719..13002)
                     /gene="xseA"
                     /locus_tag="PLG78_01145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNE21738.1"
                     /translation="MDAPARNIFSVSRVNSLIRDILQNELHLQNIWMEGEITNLVHHT
                     SGHIYFSLKDEQSTISCTFFKYANQKVREIKLANGMRIQARGGISVFAPRGSYQFNVQ
                     QVLPAGEGLLRLQIEQLKKKLHAEGLFDLSRKRPLPPFPFTLGVASAPTGAAIQDIIR
                     VARSRFPGINILLAPCVVQGPGAVESIVYAIQLLNKPEFNVDVIIAGRGGGSFENLLP
                     FNDERVARAFAGSRVPIVSAVGHEIDHPISDLAADDFAPTPSAAVLKVLPLMSEITER
                     IEECALRLRLSLVHSHTRGRRRLLQILNSKIHKKPAAMLEIWQMRMDQMHRSFNLGMQ
                     SRLHSNSRRLEKFDHFGMHFKAGLKRNEERFKLLEERLTNFSPLATLRRGYSLVRDKD
                     KKVIRSAAQVERGDEIEIMLSQGRLKAKVTEALKE"
     gene            complement(13005..14108)
                     /locus_tag="PLG78_01150"
     CDS             complement(13005..14108)
                     /locus_tag="PLG78_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE21739.1"
                     /translation="MDRPGFLNRETAFPYLSIVLMVGSSLLLVWLFSSFVWPVFLALV
                     LYVAFEGIHKKFQSITGKYKGLAATLSMLFVMIIIFGPILLLIRLLVLEAIDVTFRLK
                     DILASDDVLKMVPLLPSFADPLTSDPFFWVDLQDTVLHVVDRYTSFLDPDKLGSLLGN
                     AGSFVFGSMAVTVTIVVNLLFALILLFFLFRDGRVFYEFIEEVLPIPQDRLKIFVERL
                     GESLRAVVRGNLFISILQGCALGLGVYFCGFSRGLLFGAVAAIFSLIPVVGTAVVWLP
                     AALYLGFVENSYGLAAFMAIYGPAVYLVLENIVKPKILDRQLGIHPIFLFFAILGGLA
                     EFGITGVIIGPIMVTVFVTLWSIMPRRAPASAT"
     gene            complement(14110..14562)
                     /locus_tag="PLG78_01155"
     CDS             complement(14110..14562)
                     /locus_tag="PLG78_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21740.1"
                     /translation="MKRATLRIAFIIASMSFLSAGCGDNTSQRVVRFKDFRITQNPTD
                     LPAGLPAGFRYPDARAVLSAGYEPESYASSQEGSIEFESRDDVQHIEAYYNNAFREQG
                     WKVIQSRSEEKALLLMAESAYSEVVTVIARSGPPSRIRVYMRRLGRDG"
     gene            complement(14559..>15649)
                     /locus_tag="PLG78_01160"
     CDS             complement(14559..>15649)
                     /locus_tag="PLG78_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNE21741.1"
                     /translation="LGRKGLNLAKSGKIKQIDLKDTENKFLRPDIALFMEKSQIYQIE
                     LLLPLMRQMDIVRVKRDDVVLRNDYESILEMDPFDLQKRIMNEITAGRERRVRYEDVF
                     EPMYVPFYDKAVFDECVSAVQKRGRVLYFVILATMIREKLILSKTFRVKNFQQELADL
                     RKEVTSAFFYLQLMGLINVDYPDRKIEVSELGNNIFRGKSLVRDDQKGGIILNPDMSL
                     IAIPEKLSIRGLYLLKCFLELKSFDNVYTFQLTRDSFLEGLLLKRNPQELIDFLRTTC
                     RSDLPQNLLFSIEEWSRSVPLVTITDECVLVQTQDASHMDLLLGQISGRKFVTQKISP
                     TSILIESDKIAETIQISEKLNLIVRLIR"
BASE COUNT         3784 a         3803 c         4010 g         4052 t
ORIGIN      
        1 cagaaagcct tcgcatcctt cgcagtatca atgctccaga gtccgtttcc gtaatccttt
       61 gaagagaggc gaatctcgcc aatgtccagg taaataggca gctggctgta tctgggcacc
      121 ccgactagat ccacagaagt atcttctgcg tccacgtaga ggttattgtt gtttagttct
      181 gtgaattcga gcggagagtc ggatgcgtac gtgcctttca ggatcaatac agtcttgctg
      241 ttcatcccca ggaggctgga cagaagcggt tccgcattct tacccttgca tgatgccacc
      301 atcaggagtg cgatcaatag taacgttaag cggcatctct tcatataaac ctcacagaat
      361 aaaagaagtc atcagcccaa agtggaagaa atgaccgttt cgcttcttga aatattgcgt
      421 cttctggtac cttggatctg ttgttccagt tgtgatgggt aagagaggtt gctctggatc
      481 tttcagatgt ttttcgtaaa ccagattgta gtctatgcga agaccgattc tgaatctgcg
      541 cccggcaagg atcgaaaact cagtgccaag gtatccaaga ggttcccagc catgtctgtt
      601 tatagggcgc accagtacgt atgctgttcc accgccagcc tttaagaaaa cctgcatacg
      661 aatcgggagc tggattggat aactcagcgc tgcgtaaatt gggatgacag ttgcgctttg
      721 agtttcgcgc gaaaaatagt tcgaataaga cgtcccgaat tccgtataca gaatccaggg
      781 ccaggtcatg cgatagaaca gacctccacc caggttggaa tcgagtgctg catccagttc
      841 agtgccaggg agggggttgg atgctccgat ccacagccca atctcatgct tcacgcggga
      901 agcagactgc tgctcctggg catgaaggct gcttgcggct gtgcccgctg cgagcaaaac
      961 gatgcagatc aggattctac tctttcgcat ggttttcctg gtcaccttct cgggaactcc
     1021 tcattctgcc agcatgaatc ctcatgagcg gatgatcatc cggaagagcc agttccttga
     1081 gtccgggggc tgttccgggg acataaaacg catgtctgaa atacaagccc tccgacgggg
     1141 ccgtctgtcc tgtcttgaaa cgggatccag acagaactgc gtctgtaata gaggaaagct
     1201 ttcctgctgc tcgatctaca attgttccgg cgaggattcg aatcatgttg tgcaggaatg
     1261 cattggcgcg aacacgcaga acgatcatat ctccgtgccc ggtccatgga tcttcttttc
     1321 tgtagagaac ggccgaatcg agatatcgaa ccgttccttt tccttcgtag agtgtttttg
     1381 tcagagcaat gaagtctcta tgaccgcaaa tcgccgcaag ctcttgattg atttcttcta
     1441 tttcaaagcc tttctttctg tgccaggacc ttccgcgcca gaatggactt cgcgcgccaa
     1501 tgtgatacat gtattcatat tcgcgagcga tgcaagaaaa ccgaggatga aagttgcgcg
     1561 gcatgttcag cgatttctga acggaaacat caaaaggcag cagcgaattc agggaataag
     1621 tgagcttccc aggatccggc gcaacaggag ataagaatgt ggccacctgg cctgttgcgt
     1681 gaactccgct gtctgtcctt ccagcgcaat ccactcgcgt atgttcgcgt acaacaatct
     1741 gcaaagcatt ttccagcaac tcttgaatgc ttgttccgct cttctggtat tggaagcccg
     1801 catattttgt tccgtcgtac gaaacgataa gcgcagtgcg gtgataatca ggatgcggct
     1861 ggagcagtgg gcggaacccc gttgattgta tctatttcct tctgcaattc ttcatagagt
     1921 tctttggcca tgtctatgat gacggaaggt ttgctcttgg atgcgcggcc catgccgtat
     1981 agttttccga ggtagcgttt gcttcggtcc aggtattcga gcttcttttc ttttgtgggt
     2041 gcaagctgat tgaggtactt gcgcgtcagg tacccgttga tatagataac accatcaaaa
     2101 ctccagttgt tgtccgtatc cggaccaaga aggttgatga attgctcatg aggttcgcgg
     2161 ccattcgtca tgatttcgag agtggggatg taccagctga ctgacttaag atagaggaat
     2221 tcgctcagtt tctcaaaacc gtactttgga aattcttccg ccatgtcctg gaaaagccag
     2281 gcaccccgaa gtgcgcagat tgcttttttg ggagtgggtg cgattcccat cccccgcatt
     2341 tgatagcagt ctatggccag cagatagctg gccgcgccgg aaaccagatt tcgatcgtct
     2401 gcgaaatcaa cgggccccac aatcttctcg acagacgtac gcctgtccag agtggccttc
     2461 tttagttttt ctacttcatc cggcggaaga agagcaaaat cagttggaaa agaggagaac
     2521 aggcactgtg gacaggtcag gatggaataa gccagtggat acaccttgcc atatcttttg
     2581 ctcacctcgt agagccggcg aagctcctgg gtgagtttcc cggcgatcaa gcggcccccg
     2641 cccgaatgaa gttgttcccg gttgaattcg aagccacaga ccttgcaggt gctcttgtct
     2701 ttgacccgat aggaaatggc tttagccttg gatatttcgg gctgtttgat gcgcatcggg
     2761 ttttacacgt tggcttgccc gtgccggcgt gtaaatttta ttttggcctg cgtcaggttt
     2821 gaacttgcat ccaccgatag gaataagagc ttactcgagg gttcttctca ttatgaagcg
     2881 cagactggca tttttactac ttttatgtct ccccgtggcg atccatgcgg agtcggtggc
     2941 tgaaaaagcc accgaaaagc gaaacgagct gattcgctat ttgcgcgaaa tccgcacgat
     3001 ggcttacaac tatccctgcg atccattccc ggattgccag gccgcccttc ctcaggggga
     3061 tgacaagaag gctccggcaa cacctgcagg aaccactgcg cagccagaga gaatcaagct
     3121 ctttggagat atcaagcgca tctatcagga aggcatggtc tacctgtatg agggcaattt
     3181 tgtgaattca tacaatcggt ttctggattc tcaggctcgc gtggagagaa tgctcgaagg
     3241 tcttagccag ctctacctgg atcgtgcgga agcaatgatg cgtgattcta tagaaaagaa
     3301 agatccaacg gatcccgcag acatgactgc gactgacatt tccattgact acggtcagaa
     3361 gtcagataag cgtcgcgatt tcgcgcgcaa tcgtgaaatt cctcgcgatt cgagaacgta
     3421 tgatcaaaaa gaagtgcact acgcccataa caaatacgag atcgaacgca atattgaaat
     3481 gggatacaag cacctgggcc tcgcacgcga agcacgccgt cgcgctctga tcgtggatac
     3541 caatctagct cccaatcaac gcatggaacc gcatcatcgt cgtgcacgaa tcgaatacta
     3601 cctggattgc attcggatgg ctcgtcttgc gaaactgaac ggagaacgta tctatcagtt
     3661 gaagtatccg tacgacaact atgcattgat tagccctttc ggaaaaacag aaatcgtgag
     3721 aggggatcat cctaaagaag cgaagttccc tgtcattgcg ggtaaagaga gagactggtc
     3781 tacagatgaa aacactgcag acgcaaacaa gaaagcagac aagtccaatc cttacatcta
     3841 tcctgcgaat ttgaaccctg tatttgatct tcgcattcca gagaagtatc gtcgcgattc
     3901 gtctgatgcg cgcgatcaaa tctatgcaga tcaaatagat atcaatgttg atttccgcta
     3961 cattcctgaa aagaggaaca agcctactgc ggactcaacc acgccggcca aacaaccttg
     4021 atttggattg cgctgcccgt gtgaacggca gcgcatcatt cattcgattg agaagccccg
     4081 ccatggcggg gcttttttaa tttccctgcg attcgaactc tttgcgcgca atcagcacct
     4141 ggttcatttt cagttgagcc agtcgacgca gttcgatcat ttcttctttg tgtttaacaa
     4201 gttcgggatc ttcttcaaat ctaccttcca tcattttcag tgtatctctc aggaatcgaa
     4261 gatctttttc tgtagcgatt ttgtgctgtt tgatcagcgt tttgatttgc tcgaattcgt
     4321 atttgcgaag gtggatgcga accagaaatt gaacaccgaa cttatccacc gcttctcccc
     4381 actttccaga gcggttcatc tcttcgattt tttgcagatg ttccgagtca atcgctcgac
     4441 gtggttgtgc ttccgttttt gcggacgagg cagttgcact gcctgatcca atatcaaagc
     4501 ccacttcggg aagtagttcg ttcagtttct tctgtttcca gccttccgag aattctgcga
     4561 atcgagttgc catgaactgc tcgtattgct ggagcatcat ggccaggttc atgctttttc
     4621 caagaggggt attgttgaac tggcgagcgt tgccaatcag ttccctcaga atcaacgttg
     4681 cctgttttct tttgtaatac tggccttcgt aggagagctc cagttccgag taagatactt
     4741 ccttcaatcg catgatgact ggaacatcgc agacaacgta tgcttctagt ccatgaatga
     4801 gaagtcgctc atctggttcc acgagtctga ttgtgaaaag gtagtgcgca tctgaaagca
     4861 cacgaagata gaatctcatg cgatcttcgc tttcgcacaa atcgctcagc agctttcggt
     4921 agattgtgtc tgaaaacgga atcgatgtcc gctttccttc ctcgtttaaa gtccgaattc
     4981 ttcggacgat ctccatgagg cgttcgccaa gggtcactct aacattccct tcttgcgaaa
     5041 gatggcggtg atgttctgca gatcttcggg tgtatccact ccaggtagcg aacctttcac
     5101 aatgtgtgcg cccatcgagt aatcgttctc aagtactcgg agctgctcga gagattctgt
     5161 atcttccaga ccagaaccgg gcagcgtgcg aaagcgaagc agaaaatccc gacgatacgc
     5221 gtagattccc agatggagaa aaacaggatg tcccgttttg tttcttggaa atggaatgag
     5281 cgatcttgaa aaatagagca ctttaccttt cttggtgagt gttaccttga cgcggtttgg
     5341 aatgagcgga tcttccgcat cttcaaaggg gcgagcagct gtagttactt cccattcagg
     5401 atggtctttt aagagctgaa cgactccaga gatcaggtct ggttcgatgc ccggttcgtc
     5461 tccctgaatg ttaacgacaa tctgatagtc tttgtatgat tccgaaactt ccgcgagtct
     5521 gtctgagccc gccttgtggt cttcagatgt catcagcact tctccgccaa acgccttcac
     5581 tgcatcccgc actctttcat cgtctgttgc gatgacaaga tgatcaagat ccttgcaccg
     5641 cgatgcggct tcgtagaccc actgaaccat tggctttttg ccgagcggtg caatgacctt
     5701 acccggaaat ctctgagaac cgtatcgcgc tggaatgacg ccaagtactt tcatctaaat
     5761 ttcaggctgt gagagggggt tggtacgtca atcagctctt cagtgggtcg atcaggcccg
     5821 cctccgcaaa tcccttttcg cgcaaaacac aactgtcgca caatccgcac ggttttccag
     5881 aagcggaagg ctggtaacag ctagaggtcc tggagtagtc tactcccagt ttcattcctt
     5941 cttgaatgat ccgcgccttg ttccattgca tgagtggcgc gtggattatg atgggccttc
     6001 cttccacacc ccgtttcatt cccagtgctg ccatttgttc gaatgcctga atgaattctg
     6061 gtctgcaatc agggtatcct gaataatcca gcgcgttgac cccgatgaaa atattcccta
     6121 tgtcattgga ttcgcatacc gcgagagcgt gagagagaaa tagtatgttt cgcgcgggca
     6181 cataagtgac agggatagaa tcatcgattt gacgtttctc gggaacggca gggcctcccg
     6241 gcacgagagc cgtatttttg aagagttctt gatcaatacg tagtactaaa tgttttgcgt
     6301 tcgccctgat ggcaagctcc cgcgctcttt ccagttcgaa tttatgccgt tgtccgtagt
     6361 caaaggataa tgctatcaag gagtatccgt gcgatttcgc aatctgcaat acagttgcag
     6421 agtcgagacc gccactcaga aggactactg cgccttcagc ctttgtagat acatcttgca
     6481 ttgcacgttt cgtgaacaat aatttccgtc aaaaatggca tggaaggttt gagtctcttc
     6541 cagagccaga tcgccacttc ttcgctcgtt gggttttcga ggcctggaac ttcattgagt
     6601 agattgtggt ccagatactt ctcaaggagc ggctttacaa cggaagtgat gtctccgaag
     6661 tccatgagcc atccggtctg cgggttcact tctccattga gttttatttc gaagcgaaac
     6721 gagtggccgt gcatgcgtcc gcatttgtgt ccttctggaa ctcgtggcag gaagtgagct
     6781 gcttcaaacc gaaactgctt cacgatttcc actacgcgtg actccgcggg tttatgcagt
     6841 gaatcaaact cctgttgcat tcgctcccca gatgatctta tgaagctgga tctgcatgcg
     6901 tgcgtcaatg ttttcttcaa gcaaccattg cgtgagagtt gctggagcca cgcttcccca
     6961 tacaggggaa aggagtagat ggcacacgtt ctctagattg tgagatctga tccaggaaag
     7021 tgcggcgtca aaatcgattc tatcctgaat cacgaacttg atttcgtccg acttcttgag
     7081 ccactcgaaa tttttctcga atggatggtg agcctgtccc gatcccggaa gcttgatatc
     7141 catgacaatg tggacgtagt ctggaattcc atccaggctt ctgtgtccgc ccgtctctag
     7201 aagaatcttt tttcctctgg catgcaaagc ttcgcagagc gcaatcacct ggtccttttg
     7261 caccagtggc tctccacctg tgatctggat caggcgaacg gatgtgggga ctcgtgcgag
     7321 taagtcttca aaggagatgg aaggtccgtt tccaagcgcc tgcggagtgt cacaccagga
     7381 gcatcgaatg tcgcaaacag cggtacgaat gaagaagcac ggtaatcctg catgcattcc
     7441 ttctccttga acggaaaggt aagtctcaat gatgcggata tttttgtcct ggctcatgct
     7501 aaaagggttg agcacagaga atgcgccgta gcctcacgtt gtgctggttg ttcggttcgc
     7561 acagatcgtt ttcctggcgg gtcttgtcgc gggaatcgtt tttctattct ggtcctggcg
     7621 tcgtggagtt cgtttcatcc aaatagaacg cgccagattg aggcgagtgg atacggtaga
     7681 atggaaccct tccattccgc gtgattttct gaatgaggtt ctatcacccg atcgcatcga
     7741 attgtgctct attgcgcagg atgcgtctct cgtgcgaatt tgccagtgga tggccgagca
     7801 gtcattgtct gtggcagcga ttccggcgcg tgggccgagt ggatcgggtg tttgcattct
     7861 tcggccgggt gccctgcagg atgggtttgt ctggcggctt tttttcgatc gtaaccttcc
     7921 gcgaccttcc actctggaag cagtagtagc caggatggat cttttcgtga gatccggtga
     7981 aaaagcggaa tcacccgcga gcgcgcctgg cgaaacggcg gaatacagat tgagtcagtt
     8041 gcgcgcactg gagctgtccg ttcaacgcat cgctcatgac attcgcattc cagtggcctc
     8101 actgtcattg attggcgaaa accttggcgt agagtgggag agcatccgac ggacggaagc
     8161 ttccgttcat atggatggac tcttgattcg tctcaggcga cagattgcga atctagaatt
     8221 gttcgcgcgc ggctttctag atctggagaa aggggcccag ccggcgtcag aagtgaagac
     8281 actggatgtg ggggctctgt ggctctcgct cctggatgca catgcggaag agatgaagaa
     8341 gaagagtctt cgattgaaaa tcgcgggaga tatgacagga caaatggttc gatgcgcaga
     8401 aagcgatctg gtccgaattc ttgaaaacct tctgtccaat gctttgaagt tctctttgcc
     8461 gggtggttgc atctggactt ccgtgagggg tgaggaatcg gacattgtgc tggatttgga
     8521 ggattgtggg ccaggattca ctgccagtac cgaaatggat ccttttgatc tggctcgcag
     8581 aaatctggga agaacaggga caggatacgg gcttggcctc gctgcagctc tcgacctggc
     8641 agaatcaatg aatgggaaac tttccctcgc caatcccctt cacggcgccg gcgccagatt
     8701 tttgcttgta cttccgggcg cggagttgtc tctggacaca tgaccggcat ccaccttcgc
     8761 aacacgacgg gcaagagatc aggttccttt attcgctacg aatacgggga ggatttgttt
     8821 ggctacgtgt atctcgacgt gatccgcggt cgtcgtcatc gtgctcaaaa ggtttggact
     8881 gcactgttta ccagcaaaat cgatttcctg atcactctgg atatggacct ggaaagaaaa
     8941 gagaacctga actacgtcca cgcctgacct gctggatttt tcctaacaaa tcgcacttga
     9001 aaaaaaatga cccgccattc acggtcgggt cgtggaaaac agtgttttcg tacttggctc
     9061 cgggcccctt ggtttgatcc tttcttctaa tctatctgag aaggcttctt ccgtcactct
     9121 atgctggcat gattctgcgg aagcggcgcg aatgcgccgc gacggtggca cgcgcttgct
     9181 caatcaggat ttctttttcc cggacaatgt ggagatagcg gatcgaactg cctatcttcc
     9241 agacgattgc attctgatcg ttgcgatctc ttctcgcgaa ctcgaagaaa ccatggaggc
     9301 catgcttgca tctttcaaag atcgctcagg tctgatcgtt ctgtttacta aaggacttcc
     9361 atcttacact gcacgaaaaa aatacaacgc gcgcactttc tgtgaataca tggaaaaatt
     9421 ttcgcatgat cgcggcattc atcagcttaa atttgccgcc gtcaacggac ccagtttact
     9481 ctccgaactc tatcatggat cctacagctt tctgaacgta ggctgcgccg acacagacgc
     9541 ggggaaacga atcgcggagt tgctttctaa cgatcgcatc cagtgcggtt acacctctga
     9601 tacaatcggc gtggaaatgg gcggcgtgct caaaaatcca atcgcgattg catctggcat
     9661 tgcaacgaac ctggcggaat gcggaggcaa cttcgcaggc gagctcatac aacacggctt
     9721 ccaggaaatg ctttcccttt ctctggcaat tggcgctcgc caggagactc tcatcggaag
     9781 atcgggcatt gcagacttaa tgacaacgtg cttgagcaag gaaagtcgca atcgggctta
     9841 tggtgaagat ttcatcaaac gcctgcaatc tggttctgaa gaacatgggg tgatggatcg
     9901 cattgaaaca tttttgaatc cagcgcgggt gatccagaag gatgcggctc aaggtcagga
     9961 tgtagtagaa ggcgcctatg cgattgcttc tattgtagag attgcagctg agttaaaagt
    10021 agaggttccg ctttacaacg cgttatacga gattctgtcg cgcagaagtc ccccgaacgc
    10081 actcatatcc gcgattacag gtgcgcccgt tcgagcaagt gagccgcagg ttctgcatag
    10141 aaggcagggt ttgcacctgg ccgccgggac agatttcgta gaggtactct cttcgcgtgt
    10201 ttttcatagc gttcaggtga ccaaaggaat gcaatctcgc atcaagaagc aagcgacgca
    10261 agtactcact caactggaca agagacttca gaaagcaaag aaagtgaaga atcgtatgga
    10321 tcttcagaag attccgcacg aaagggattt gtggctgtct ttctcttctt cgataggtga
    10381 atcagaaagc cgcgctctac gacatttgat tcaattctac gcggaagaga tcgcagacag
    10441 ctacactcca ggcatgcgcg agactctcat tcgtcttctt gcccccgtgc gatttgctgc
    10501 gagtggtttc aagctgggat ctgccatgcc aaaaattggt ggaaacacgg aggagttgaa
    10561 gaacctcgca gcacactaca acgtgatgta cacccccaca catcgatcac acctggattc
    10621 agtagaagtt gcctttggac tttcctggct ttctgttcct cttccgcggt acgccgcagg
    10681 aatcaatttg atggcggatc aattccaatc gtggttgctg cgttcttttg gagcctacgc
    10741 cgtcgatcgg gaacggacgc gaaattttct ctatctcgaa tgtctttcgt cttacgcttg
    10801 tttgatgctt gagtctggca ttccatccct ggtttatccg gaaggaacgc gaagtcgcga
    10861 cggaaaaatc gttggaatca agacaggtct tttgtctact gctgtctcag cctatcagaa
    10921 cacgggccgc gaagtcgttg taatgcccct tgctctttcc tatgaaagca tccccgaaga
    10981 ttcattctat tgtggactcg gcgaagaacc tggcctgaag gaattcatca acaaaagagg
    11041 aagtgcatac ctggacattt gtgatccaat tcgagtgtcc aggcacatgt ctgcggatga
    11101 tccaactctc tccatatcca tggcgattgt agaacagtgg aagaagcatt tgcgtatctt
    11161 acccaaccag attctcgcac gcattctatc tgacaatggc cacgcggtgc cacgtgccgg
    11221 cctccccgct cttgtggaag agttcgtgct gcgaaatcca gggaactatc tgacgcgcga
    11281 tgcggaggcg attgtggagc aaggcattgc atcgctccgg gagcgcaata ttgcgaagga
    11341 agatggtgtt cagctggcat ctgttacgcc cgctctcgcc tcgtattacg gcggtatggt
    11401 gccggaatcc ggaggaacga tcctggacca ggacgcgtga ttttttggcc acattgattc
    11461 gcaaaagtac ttgcaatctt ttacaattcg ctgacactgt gtcagggaat tcgctttccc
    11521 agggggactc aaatgaaaca gcttttaggt gtcgtggcta tggccaggtc ctcgcagtga
    11581 caatttcaag aatttgcata agacgcagtc ttataaggca agcgatttca catgtgctgc
    11641 ctttgaagac gcagagctgc ttgcaaaagc aaaactcttg gagcaagagg cggcggaaga
    11701 aaagcaatag aagggtggtt attctttcaa ggcttccgtt actttcgctt tcagtctgcc
    11761 ctgagagagc atgatttcaa tttcatcgcc gcgttccacc tgcgcggcgg atcgaatgac
    11821 ttttttgtct ttgtcccgaa ccagagaata gccgcggcgc aatgtggcca gaggactgaa
    11881 gttggtcagt ctctcttcca gtaatttgaa tcgttcttcg tttcgcttca gtccggcttt
    11941 gaagtgcatc ccgaagtgat caaatttttc cagtctgcga ctgttgctgt gcaatctgct
    12001 ttgcataccc aggttgaacg atcgatgcat ctgatccatt ctcatttgcc agatctcaag
    12061 catggccgcc ggttttttgt ggattttgga gttgagaatc tggagcaatc ttcttcttcc
    12121 gcgtgtatgc gaatgcacga gggaaagcct caatctcaga gcgcattctt ctatgcgttc
    12181 tgtgatttct gacatgagcg gaagaacctt gagaactgca gcgctgggcg ttggcgcaaa
    12241 atcatctgct gcgagatcgg atattggatg atcgatttca tgtcctactg cggatacgat
    12301 gggcactcgc gacccggcaa atgctcgtgc cactctttcg tcgttgaagg gcaaaaggtt
    12361 ttcaaaggat ccaccgcctc tgcctgcgat gatcacatct acgttgaact ccggtttatt
    12421 caatagttga atcgcatata cgatggattc cacagcgccc gggccctgca ccacacaggg
    12481 cgcaagcaag atgttgattc ccggaaaccg agatcgcgca acgcgaatga tgtcctggat
    12541 ggctgcccct gttggagcag aagcaactcc cagtgtaaaa ggaaaaggag gcagcggcct
    12601 ttttctcgac agatcgaaca agccctccgc atgcagtttt ttcttcaact gttcgatttg
    12661 aagacgaagc aaaccttcgc ctgcaggaag tacttgctgc acgttgaatt gataagaacc
    12721 gcgaggagcg aatacagaaa ttccaccgcg tgcctggatt cgcattccat tggccaattt
    12781 gatttcgcgc actttctgat tcgcgtactt gaaaaatgtg cagctaatgg tggactgttc
    12841 gtcttttaga gaaaagtaaa tgtgaccact tgtgtgatgg accaggtttg tgatttcgcc
    12901 ttccatccag atgttctgca ggtgaagctc attctgcagt atgtcccgaa tgagagaatt
    12961 tacgcgcgaa acgctgaata tgtttcgagc aggtgcgtcc atgtttaagt cgctgaggcg
    13021 ggagcgcgtc tgggcatgat gctccagagc gtcacaaata cagtgaccat gataggtcct
    13081 atgatcacac cggtaattcc aaactcagca agtccgccaa ggatggcgaa aaagagaaag
    13141 atgggatgga tgccaagctg acgatccagg atttttggct tcacaatgtt ttcgagaacg
    13201 aggtaaacag cagggccgta gatggccatg aacgctgcga ggccgtaaga attttctaca
    13261 aaaccaaggt agagagccgc cgggagccat actacagcag ttccgaccac tgggatgaga
    13321 gagaagatgg ctgctacggc gccgaataaa agtccgcgcg aaaatccgca gaaataaaca
    13381 ccgagtccaa gggcgcatcc ctgcaagatt gagatgaaca aattcccgcg aacgacggcg
    13441 cgaagcgatt cgccaaggcg ttctacgaaa atcttgagtc tatcctgcgg gatgggcagg
    13501 acttcctcaa tgaattcgta gaaaactctt ccatctcgaa atagaaaaaa caaaagtatg
    13561 agcgcaaaca gtagattcac tacgatcgtt acagttacgg ccatgcttcc gaaaacaaaa
    13621 ctgcctgcgt tgccaaggag gctgcccagt ttgtccggat ccaaaaagct agtgtaacga
    13681 tccactacat ggagcactgt gtcctgcaaa tcaacccaga agaaaggatc cgatgtgagt
    13741 ggatcggcga aggaaggaag tagcggaacc atcttcagta catcatctga agccagaatg
    13801 tctttcaggc gaaacgtaac atcaatcgct tccaggacaa gcagtcgaat caaaagcaag
    13861 atggggccaa agatgatgat catcacaaag agcatgctga gagtggccgc aagccctttg
    13921 tattttcccg tgatgctttg gaacttcttg tgaattcctt caaaggccac gtagagaacg
    13981 agcgcgagaa agaccggcca gacaaaggaa ctgaataacc agacaagaag gagggacgag
    14041 cctaccatca gcacaatgga tagataggga aatgcagtct ctcgattgag aaaaccgggc
    14101 cgatccatgt tagccgtctc tacccaggcg tctcatgtag actcgaattc tggaaggtgg
    14161 gccggatctt gcgatcacgg tgaccacttc gctgtaagcg gactctgcca tgagaagcaa
    14221 tgccttttct tctgacctgc tctggattac tttccagccc tgctcgcgaa atgcgttgtt
    14281 gtaatacgct tcaatgtgct gcacgtcatc gcggctttca aattcaatgc tgccttcctg
    14341 ggaagatgca taggattctg gttcataccc cgcagacagt acagcgcgtg cgtcaggata
    14401 tcgaaacccc gctggaagtc cggctggaag atctgtcggg ttctgtgtga ttcgaaaatc
    14461 tttaaagcga acgactctct gtgaagtgtt gtcaccgcat cctgcggaca aaaatgacat
    14521 gctcgcgatg atgaacgcga tccgtagagt ggcgcgtttc accggatgag tctcacgatc
    14581 agattcagtt tctcagagat ttgaatcgtc tctgcaattt tgtcactttc gatgagtatg
    14641 gaagtggggg atatcttttg agtgacgaac ttgcggcctg agatttgccc gagcagaaga
    14701 tccatgtgtg acgcatcctg agtttggacc agtacacatt cgtccgtgat ggtcacaaga
    14761 ggaacagacc gagaccattc ttcaatggag aataggaggt tttgcggcag atcgctgcga
    14821 caggtagtgc gaaggaaatc aatgagctcc tgaggattgc gcttgaggag caagccctcg
    14881 aggaaggagt cgcgtgtaag ctggaacgtg tatacattgt caaagctctt gagttcgagg
    14941 aaacacttca atagatagag gccgcgaata gagagttttt ccgggattgc aatcagactc
    15001 atgtctggat tgagaatgat accacctttt tgatcatcgc gcacgaggct cttgccgcgg
    15061 aagatgttgt tgcctagttc agaaacctcg atcttgcgat ccgggtagtc tacattgatg
    15121 aggcccatca attgaagata gaagaatgcg cttgtcactt ctttgcgaag atctgccagt
    15181 tcttgttgaa agttcttcac gcgaaaagtt ttcgagagaa ttagcttttc gcgaatcata
    15241 gttgccagga ttacgaagta aagtacgcgt ccgcgtttct gaactgcaga aacgcattcg
    15301 tcgaacactg ccttgtcata gaaaggaacg tacattggtt caaacacatc ctcgtagcga
    15361 acgcgtcttt ctctgccggc tgtgatctcg ttcatgattc ttttttggag atcgaacgga
    15421 tccatttcca gaatggattc gtaatcgttg cgaagcacca catcgtcgcg cttgacgcgg
    15481 acaatgtcca tctggcgcat caaaggcagc agcaattcaa tttgatagat ctggctcttt
    15541 tccatgaaaa gagcaatgtc cgggcgaagg aatttgtttt ctgtatcttt tagatcgatt
    15601 tgtttgatct tgcctgactt tgccaggttc aatcctttgc gtcccaaat
//
LOCUS       DAOQJL010000014        14978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1898_length_14978_cov_4.645581, whole genome shotgun sequence.
ACCESSION   DAOQJL010000014 DAOQJL010000000
VERSION     DAOQJL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_14978_cov_4.645581"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLG78_01165"
     CDS             complement(<1..935)
                     /locus_tag="PLG78_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21742.1"
                     /translation="MSRAKSILFLTLLPSVIVLVLFAYTYAKKWTQKDEIVATATDGT
                     QIHGIRAMSGAVSGSSQSLAVFLPDPAFDRSLNSRSIETNAGAELEALVLKRGIHLLR
                     YDSRGTGRTPGVSRFVPPGILADDLMKAGAGATTVIAHGDSCATAALAWKKGMHAKRW
                     IFVSCAYSGNPVDEWAARTFYSMENSLMSEKLIQTCRKEWSTFRKDLRQTVSSKSGPP
                     PVVPAKEGESPDLMVFRGAMREMAYEQKAWTREALDFDVMRTIVQMNRKEDLVWFVPE
                     FDTVTVPSEVEAMKKAGVTPRPLFQTDYFLLQTDSP"
     gene            complement(936..2027)
                     /locus_tag="PLG78_01170"
     CDS             complement(936..2027)
                     /locus_tag="PLG78_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE21743.1"
                     /translation="MKLKLFFFPIVLFLGAFILDKQLFIGRFEDTFLRTGTFLNFDHK
                     VDMLDELAAYLIAPARKKTLVIFGNSRTMSFENSYIESKYKDWIWFNFSVPGGTTDYF
                     LFLIQEMKRKGIRPDAVVLTVTPQGFNTAPAVPMDEVLISGLSFPFIARYANRYTTDD
                     LMNYAAKKTFWTYRYRPKAEVILHRLEHDGLQMRIFRQFQFDTATRLKVSRGSVPFQF
                     QGIPAQDDEFLMAHAKNTWQDFFTPYKLSEGQFAFTDDSLRILTDARIPVILLWARVQ
                     KNLRQMKDTVVVTPRGEKPETVRSLWAPRMTALAAKYQVPFYDMNYGNSIACDHFFDS
                     SHLASVCFPEFSDYIMRAVLDQKPEGQKP"
     gene            complement(2035..3561)
                     /locus_tag="PLG78_01175"
     CDS             complement(2035..3561)
                     /locus_tag="PLG78_01175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE21744.1"
                     /translation="MLFNSFLFLIFFGFVYILYWALRGRARIAFLIGASVLFYSAWGL
                     QSEGWSGLRWTLHFVVITFLNYLLIHAMFRYPLRKKSIITAIIVIDILNLATFKYFAF
                     FRDSLHNLSLRLPEKLDALNLFLPLAISFYTFQLIAYAMDVYRGVIKEKVPVGRFFLF
                     FLFFPHFIAGPIMRASDLMPQIDKPWVDRNRMMGACWLILGGLVKKVLLADPMGQIVA
                     PVFREPDAYSGLSIILGGACFSLQVYCDFSGYTDIARGCAFLLGFDIPENFSAPFFAR
                     SARELWQRWHITLATWLRDYLYIPLGGSRRSEVRNHLNLIITFTLGGFWHGADISYIL
                     WGFFWGALLSLERFLENNMGWKTTPEKSRAGMVLKGMFMFLLFSIGALFFRTQPVRFA
                     DHSYTTWQMLGHVFGGIFTNTDGELLSTIRAAGMDPAQIGMIFGPSALELRRIGPMDT
                     IIWMFLALGFFHWVQYSPARFARFKEQPWLLGAVAALLGGILIPSLVSGTGQFIYFVF
                     "
     gene            complement(3574..4560)
                     /locus_tag="PLG78_01180"
     CDS             complement(3574..4560)
                     /locus_tag="PLG78_01180"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNE21745.1"
                     /translation="MRDPALSVLLERVLEEDKLLLTTHRTPDPDGLGAELAMQAMLQW
                     RGKDAIIVNPEPAPERIRFVDPERRALDMRNMKDRSLLDGRAIVILDNSDLRRIDDLQ
                     SYVLEDKSNLIIIDHHDGMQADQKSIFAFPNCGSSCEIVYELLEIAGFTMNPAIAAAL
                     YAGIVADTGNFRYRKTTARTHEIAAKLLRFGVNPADMAEKIFENTPLARLLLKKKLYS
                     NLIIEQGRLAYFKVRKEDLEALGLSMEDTEGLVNELIESESILTGILFTERQPQLTKV
                     SVRSRGDTNMLAAVQKFGGGGHKNACGATLYMDIDAAVKEFVPEALRCLDEC"
     gene            complement(4557..5768)
                     /locus_tag="PLG78_01185"
     CDS             complement(4557..5768)
                     /locus_tag="PLG78_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE21746.1"
                     /translation="MPGLLCGNSLRYVRANLPVLVFHCTDDELAKARITTQAELPGLE
                     KISIQGSAVHLIHRKNGAEHRWIFRKPEYFSTIDTLVYQAGVWSGSVEAMEDLALGRI
                     RLAPGVPLDACHAIRLAAEAALLDFQIEEELLGAAKQAIASREEEPTDEFLGRVFRDV
                     VSGAKPSTGFVALRDMGALSLMVPELAEGQNLSQNQYHEFDIFFHSIFACDALKEQNL
                     ALRLAALLHDVGKSKTRRVGSDGQATFHNHEVVGARQTDRLLRRLGFESDLIARVRFL
                     VRHHMFHYTPDWTDHAVRRFMRKVNVDQMRDLIAVRIADRKGSGKRAGVPRPVYDFLA
                     HMERIRAEDAQLKVTDLTLGGNELMEMGIPAGRGMGDVLKRLLEEVKSGALVNEKEAL
                     SARVREYGGVH"
     gene            5914..6978
                     /locus_tag="PLG78_01190"
     CDS             5914..6978
                     /locus_tag="PLG78_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNE21747.1"
                     /translation="MNRTDRIRTALPGFEVPAQLLQFIETASPEDLFRVNLVTLSTKI
                     NVPLDTLLSAFLKMVEAGLFNLSWEYHCTHCNAIPDFKHSFSELKAESYCPLCDLGFR
                     NTLDKNVEVTFTAHNSFVEIPSEIPERIKAEAIAAVKEKRYQMPEHFLSGLQCLVNET
                     FHQLFGEQVLSSEESLAVETITLLFTDIKGSTQMYSDLGDTKSYEIVREHFKILFQAI
                     VQNGGVVVKTIGDAVMGSFLKPVNAIRAAVQVHDDFNKREWTSIGYLQIKMGIHTGHA
                     IVVNLNDTVDYFGNTVNLAARVQASVKNHDIRFTKRVLEDSEVVSYLKERKAILHKKF
                     EMFKGINDPVEVYRFTPGAP"
     gene            complement(6979..8181)
                     /locus_tag="PLG78_01195"
     CDS             complement(6979..8181)
                     /locus_tag="PLG78_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNE21748.1"
                     /translation="MMEEVIVIGSGHLANRIRQVLEKKSYSVCHLSAEGIESSPDYSV
                     ARLQSIKTQLKHAGAERARAIYVVDSEDRRNIEFALAAMSFERDEPIFLALFNEKIAP
                     HFQINCKNLFIMNPARLAASTFADAVTQVRQAPLPAMAQKPEEGEPDSGIFNWLRSNV
                     LLTVLLSAFLLLYTAGAIFFRYSENLRWIDAFYFITTVITTTGFGDIHLRYSSDEAKL
                     FVICTMLTSVSFFSIIFALVVDKLMERRSQVLLGRKTHRLKGHVILCGLGRLGYQIAL
                     ELRRRGFQIVVIESNEHNRFLNTFRARGIKILYGDATLLRNLEMAGLLHAVALFSVIN
                     DDLTNLEIGLHARSLDPSARLILRIYDRETAEAVRRRLNIEFAYSTSAIAADEMVRAL
                     ESIELARN"
     gene            complement(8178..9017)
                     /gene="panC"
                     /locus_tag="PLG78_01200"
     CDS             complement(8178..9017)
                     /gene="panC"
                     /locus_tag="PLG78_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNE21749.1"
                     /translation="MKIIRSLAELRETLSVRDKVRSLVPTMGYLHEGHASLIRDARRE
                     SEQVCVNIFVNPLQFNNARDFETYPVSEDHDRAICEEAGADIVFVPTASEMLGAGPAL
                     QMAMPALTKNLCGPFRPGHFEGVLLIVAKLFHLFLPHKAYFGKKDYQQFRVIQRMVED
                     LSFPIDIIGCETIREADGLAMSSRNKNLKPEHREQAALMIRGFRIAEKAFNNGNANPA
                     DLAEIAQDVMLTGPDNRVEYTQVVDPETLEVLQEIEGDFVIAAAMHCGEVRLIDNLLI
                     RGG"
     gene            complement(9017..12301)
                     /locus_tag="PLG78_01205"
     CDS             complement(9017..12301)
                     /locus_tag="PLG78_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE21750.1"
                     /translation="MKFLSRASGITEKEWPRARSMMLLCFAIMAALAFGRAARDAFFI
                     KNAGADNLPIVYLLNGILVAGIAAVYSRVVHRLSLSRFMGLLCAAFVLAIVVLRLLIS
                     SGNVVMPYAVWSLAEVYNILMLMHFWTYANKVFDPREGKRMFPIVGAAGLVGMIVGSS
                     VTPMFVNAFGSNNLLLGWAGMVILAIPPLRLSAAAARKSGVILAGTDDSGETVAENWR
                     SLFQIPLVRTLAMIAFPMWITAWLVDYQFFHALDEVYKEQDSLAGFLGVFNGLSSLAG
                     LILQFFVTGRLLQTLGVGGTSILYPGVVAASAVGLCARSMMPVVPGKVYEPRSILGIA
                     GKFSDETLFGSVHDSAIAVLFSSLPDENRARARALLNGAMEPMATAVAGILLLVLGRF
                     HAPPIVFASLTLAMCLVWIGLALRIKKDYLRALVANLNSRNIDLQRNAFSLLSTNRDL
                     ETREILLAALSSNDEDMALFALELLDHPDLATALRLSAVLPHTTPRLKTALLGYLGRA
                     GFSETRSAIRTEVSDSDGPVRAAAVRALSLVGSREDVRSMARLIKDTHANVAAEAVIA
                     QLTRPGGQRTVGTKGLDRLLRSREEPDRFQAARILREVPLPAKAKSLVRLMEHSTADV
                     RNEAVRAMGMLKTREALRLLAAYLREDDVYLSAKEGLLQYGESALSALHAQLAIETEA
                     EAKISIVACIGEISSLRSIPVLAKLIVRQPILVENAAAEALTNIRMKFLVETGQDVSR
                     ADEYFTREVKDRIRACYFSIVENIEKDRQNVAILQKASDPTAVLLILDAVQRFARQRL
                     EVALKFLELIGDPRTIRALTADLQFGDARGRAEALEALEGSCTEAGVLVRVLEDDPAQ
                     ASRGTVDEVLRHILWKNHPPWITSCAVHAAGLLNMKMLEPELFSQLDAPDEMVRINAS
                     IALRKLKSKTFRLAVQKERNHKQLERERKKMDTRMERFLFLRSVPLFSEVDGVDLHWI
                     NEITHEQKFRARANIFREGDPGESLYIIISGSVRIFKGQKGKELTIDILQERDCFGEM
                     AILDQEPRSASVVAVRNTRLLVINRSDFQRLLIARPRISFALFKTMSRRVREANARLI
                     QLQKVV"
     gene            complement(12298..13752)
                     /locus_tag="PLG78_01210"
     CDS             complement(12298..13752)
                     /locus_tag="PLG78_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE21751.1"
                     /translation="MFLDQNRAPLLDALIAHHAEGVHPFHVPGHKHGVGARDLLSLFG
                     QTTLSFDLNAMHDLDDACNPVGVIDEAQRLAAHLFGADHAHFLVNGNTQGIHSMMLSA
                     VGPGEQIVLPRNCHRSAITGLILSGGLPIYVPVEIDAHLGVATGPTVQSVRATLEKNP
                     FVCALFVVNPSYYGFATDIEGMVRAAHDHRAAALVDEAHGTHLSFHPDFPANAMSVGA
                     DMSAVSMHKTGGSLTQSAILLSKGERVPHDILKESIDLIRSTSASYLLMVSLDTARRS
                     LAQDAPGQFERVLQLARFARTEINLIEGLYSPGKEICRPDSSVFNYDESKLLIHCASA
                     GFTGFEMERLLRDRFSIQVELADMNNVLALITIGTREEDILHLVGSLKTIVLGNNRKH
                     SRPTVPPPLMPDVISSPRQAFYSPKKFMPLSGSAGQISGEMLMSYPPGIPIICPGERI
                     TQDIIDYVRVLKEQGSLLQGTADPLAENIRVLGA"
     gene            13866..14618
                     /gene="gntF"
                     /locus_tag="PLG78_01215"
     CDS             13866..14618
                     /gene="gntF"
                     /locus_tag="PLG78_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis pre-guanitoxin forming
                     N-methyltransferase GntF"
                     /protein_id="HNE21752.1"
                     /translation="MSSEITLDYNEFSPQDYLNEYYTEVSDENESLLQFFHDTYSKLG
                     PVDSMLEVGGGPTIYQIISASRVAKHIDFSEYLPANLAMVRAWLDQKPGSFNWDIYFK
                     RVLEIEGRPASRENIDALAGTVRGAVRRLTQYDVFREPPLVEGEPLYDIIASNFCIEG
                     IQTDRQVFERQFGRMTSYLRPGGLLALCMVKNATYYRVGKRRFPVVPVNETIMENVLK
                     NAGLTLTHIQSIKAVDPDQGYEGMLMIRATRG"
     gene            14622..>14978
                     /locus_tag="PLG78_01220"
     CDS             14622..>14978
                     /locus_tag="PLG78_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21753.1"
                     /translation="MRFPFAVKLALSISLLAVSVTSLSVVFLYTDTRSMVIRLMAGRL
                     KDVGRTAAYMLESQDREGMKKMATVVRSKIKITAELRKTIADAKVGDTIPSPLTQEEI
                     DKIIQSPDYLEMVQFLR"
BASE COUNT         3990 a         3949 c         3571 g         3468 t
ORIGIN      
        1 ggactgtccg tttgcagtaa gaaataatcc gtttgaaaga gcggcctggg agtcactcct
       61 gcttttttca ttgcttccac ttctgacgga acagtaaccg tgtcaaattc aggtacaaac
      121 cacactaaat cttcttttcg attcatctgc acgattgtcc gcataacgtc aaaatccaat
      181 gcttcgcgcg tccatgcctt ctgttcatat gccatttctc tcatggcacc tcgaaatacc
      241 atcaggtccg gtgattctcc ctcctttgcc ggcacaacgg gtggcggacc agatttgctt
      301 gaaacagtct ggcgaagatc ctttctaaaa gtagaccact ctttgcggca tgtctgaatc
      361 aacttttcag acatgagact attttccata ctatagaagg tgcgggcggc ccattcatcc
      421 acagggtttc cactgtacgc gcacgacaca aagatccatc tcttcgcgtg cattcctttc
      481 ttccatgcaa gcgccgccgt ggcgcatgaa tccccatgag cgatgaccgt tgtagcaccg
      541 gcccccgcct tcatcaaatc atccgcaaga atcccgggag gaacaaatcg cgagactcca
      601 ggagttctac cggtgccgcg cgaatcgtag cgcaataaat gaattcccct ctttagaacg
      661 agagcttcca attctgcgcc cgcattcgtt tcaatggagc gactgttcaa ggaacgatca
      721 aaggcaggat ccggtaagaa cacagcgaga gactgcgaag agccagagac cgcaccggac
      781 atggcgcgaa tgccgtgaat ttgtgttccg tctgtggctg tcgcaacaat ttcatccttc
      841 tgagtccatt tcttcgcgta cgtatacgca aagagaacga gcacaatcac ggacggcaga
      901 agcgtcaaaa agagaatgga ctttgcgcga ctcattcagg gcttttgtcc ctccggcttc
      961 tggtccagaa cagcgcgcat gatataatca gagaattccg gaaagcacac ggatgcgaga
     1021 tgactggaat caaagaaatg atcgcatgca atggaattgc cgtagttcat atcatagaac
     1081 ggcacctgat actttgccgc gagcgctgtc atgcgaggag cccacagaga acgaaccgtc
     1141 tctggcttct ctccacgcgg agtgaccaca acggtgtctt tcatctgcct tagattcttt
     1201 tgcacgcggg cccagagcaa aatcaccggg attcgagcgt ccgtcaaaat gcgaagagaa
     1261 tcatccgtaa aagcaaattg tccctctgaa agcttgtagg gagtaaaaaa atcctgccat
     1321 gtattcttcg cgtgcgccat caagaactcg tcatcctgcg ccggaattcc ctggaactga
     1381 aatggaacgg atcccctgct taccttcaaa cgagtggcgg tatcaaattg aaactgtctg
     1441 aaaatcctca tctgtagtcc atcgtgctcg aggcgatgca ggatcacttc cgcctttggt
     1501 cgataacgat atgtccagaa tgtcttcttg gccgcgtaat tcatcaaatc gtctgttgtg
     1561 tagcgattgg cgtatcgcgc gatgaacggg aaagataaac cactgatcaa cacttcgtcc
     1621 atgggcacag caggagcggt gttgaaacct tgaggagtta cagtcaatac gactgcatcc
     1681 ggtcttattc cttttctttt catttcctga atcaaaaaca ggaagtagtc tgtggtccca
     1741 ccaggtacgg agaaattgaa ccagatccag tctttgtatt tggattcgat gtagctgttc
     1801 tcgaaagaca ttgtgcgaga atttccaaaa atcacgagag tcttctttct agcgggcgca
     1861 atcaggtatg cggccagctc atcgagcata tccactttgt gatcaaagtt cagaaatgtt
     1921 cctgttctca gaaacgtatc ttcgaaccgt ccgataaaca gctgtttgtc gaggataaac
     1981 gctccgagga aaagtacgat agggaaaaag aataatttca gcttcataaa tccctcagaa
     2041 tacaaaatag ataaactgcc ccgtcccgct caccagagac ggaataagaa ttccaccgag
     2101 caaggccgcc accgcgccca gaagccaggg ctgttcttta aagcgtgcaa aacgcgccgg
     2161 agaatactgc acccagtgaa agaatcccag agcaagaaac atccagatga ttgtgtccat
     2221 cgggccaatg cgacgcaatt cgagcgcgga aggaccaaag atcattccaa tttgagccgg
     2281 atccattccc gcagcacgaa ttgtggatag aagttcgccg tctgtatttg tgaaaatccc
     2341 tccaaataca tgtccaagca tttgccacgt tgtgtagctg tgatccgcga aacgaacggg
     2401 ctgcgtgcga aagaaaagcg ctccaatgga aaacaaaagg aacatgaaca ttcctttcaa
     2461 aaccataccc gcgcgcgatt tctccggagt agtcttccag cccatgttgt tttcgaggaa
     2521 gcgctccaga gacaacagcg caccccagaa gaatccccag agaatgtagc tgatatctgc
     2581 gccgtgccag aaaccaccca gagtaaaagt aataatcaaa ttcagatgat ttcttacttc
     2641 actgcgtcta cttccaccca gaggaatgta gagataatct cgcaaccacg tagccagtgt
     2701 tatgtgccag cgctgccaga gttctctggc acttctcgca aagaaaggcg ctgaaaaatt
     2761 ttccggtata tcaaacccaa gaaggaaagc gcaaccgcga gctatatctg tgtatcctga
     2821 gaaatcacag tagacctgca aagaaaagca agcaccgccc agaatgatgg aaaggccgga
     2881 gtaagcatct ggctctcgaa atacaggcgc gacgatctga cccattggat ctgcaagcag
     2941 caccttcttc acaagaccgc ccagaatcag ccagcaggcc cccatcatgc gattgcgatc
     3001 cacccagggc ttatcaattt gcggcatcag atccgatgca cgcataatgg gtccggcaat
     3061 gaagtgagga aaaaacaaaa agaacaaaaa gaatctgccc accggcactt tctctttgat
     3121 cactccgcga tacacgtcca tcgcatacgc aatcagctga aaggtgtaga aggaaatcgc
     3181 gaggggaaga aacagattca gtgcatccag cttttctgga agtctaagag aaaggttgtg
     3241 gagtgaatct cgaaagaatg cgaagtactt gaacgtagcc aggttcaaga tgtcgattac
     3301 gatgatcgcg gtgatgatgc tcttcttacg aagagggtat ctgaacatcg cgtgaatcaa
     3361 caaatagttc aggaaagtga tgactacgaa atgtaaggtc cagcgaagac cactccagcc
     3421 ttcactctgc aaaccccacg cagaataaaa aagaacggat gcaccaatta gaaacgcgat
     3481 tcgggcacga ccgcgcaaag cccagtatag aatgtagacg aagccaaaaa atatcagaaa
     3541 taaaaacgaa ttaaagagca tcgcaggtca aaatcagcat tcgtccagac aacgcaacgc
     3601 ttctggaaca aattccttca ccgccgcatc gatgtccata taaagcgtcg caccgcacgc
     3661 attcttgtgc ccgccgccgc caaacttctg cacggctgcc agcatgtttg tatctccgcg
     3721 cgatcgcacg gagactttgg tcaactgagg ctggcgctct gtaaaaagaa tcccggtcaa
     3781 aatgctttcc gattcaatca attcattgac caggccttcc gtatcttcca tgctcaagcc
     3841 gagcgcctca aggtcttctt tcctcacttt aaaataagca agccttccct gttcgatgat
     3901 tagattgctg tagagtttct tctttaagag aagtcgcgca agcggagtat tctcaaaaat
     3961 cttttcagcc atgtccgcag gattgacacc gaaacgaaga agcttggccg cgatttcgtg
     4021 tgtgcgcgct gtagtcttgc gatatctgaa attccccgta tccgcaacaa ttccggcgta
     4081 gagcgcggca gcaatggccg ggttcatggt gaatccagca atctcaagca actcataaac
     4141 gatttcgcag cttgatccgc aattgggaaa agcgaagatt gatttttggt ccgcttgcat
     4201 tccatcatga tgatcgatga tgattagatt ggacttatcc tccagaacat agctctgcaa
     4261 atcgtcaatg cgcctcaggt cagaattgtc cagaatcaca atggcccgac cgtcgagcaa
     4321 cgaacgatcc ttcatgtttc gcatatcgag tgctctgcgc tccgggtcta caaagcggat
     4381 tcgttccggc gctggttctg gattcactat gatcgcatct tttccgcgcc actgcagcat
     4441 tgcctgcatg gccagttccg ctcccaatcc atctggatca ggagttctgt gcgtggtcag
     4501 aagaagcttg tcttcttcca ggacccgttc gagcaatacg gaaagagcag gatctctcaa
     4561 tggacgcctc cgtattctct tactcgcgcg ctgagggctt ctttttcgtt tacaagcgcc
     4621 cccgacttca cttcctcgag gagccttttc agcacgtctc ccattccgcg acctgcagga
     4681 attcccatct ccatcagttc atttccgcct aacgttaagt ccgtgacttt gagctgtgcg
     4741 tcctctgcac gaattcgctc catatgggca aggaagtcat acacgggtct tggtacgcct
     4801 gcccttttac cacttccttt gcgatccgct atgcgaactg caatcagatc ccgcatttga
     4861 tccacattca cctttctcat gaatcgcctg accgcatggt ccgtccaatc cggagtgtag
     4921 tgaaacatat gatgacgcac aaggaaacgc actctcgcga tcaaatcaga ctcaaatcca
     4981 agacgacgaa gcaatcgatc tgtttgcctg gcgcctacta cttcatgatt gtgaaacgtt
     5041 gcctgcccat cggagccaac gcgacgagtt tttgattttc ctacatcgtg caagagggct
     5101 gccaggcgca gtgcgaggtt ctgttctttg agcgcgtcgc atgcgaaaat actatggaag
     5161 aatatatcga attcgtggta ttgattctgc gaaagattct gcccttccgc gagctcagga
     5221 accataagag aaagagctcc catatcacgc agcgcaacaa atccggtgga aggctttgct
     5281 cccgacacaa cgtcgcgaaa gactctgcca agaaattcgt ccgtcggttc ttcttcgcgc
     5341 gatgcgattg cctgctttgc tgccccgagc agttcttcct cgatttgaaa atccaggaga
     5401 gccgcttccg ctgcaaggcg aatcgcatga catgcgtcga gaggtacgcc tggcgcaaga
     5461 cgaatccgcc ccagtgccag gtcttccatt gcctcgacag atccgctcca aacgccggct
     5521 tgataaacca gagtgtctat tgtggagaaa tactcaggct tgcgaaagat ccatctgtgc
     5581 tctgcgccat tcttgcgatg aatcaaatga acagctgagc cctggatgga aattttttcc
     5641 agcccgggaa gttccgcctg ggtagtgatc ctcgctttcg cgagttcgtc atccgtgcag
     5701 tggaaaacga gtacgggcag gtttgcgcga acatagcgaa ggctgttccc gcaaagcaaa
     5761 ccgggcatat gtagctcctg ggccagtaga gctgagtggc tttctgaaga tggagcctgc
     5821 ataccgttgt actgaaagat atgaacctgc aagtaccggt gtaaagcaga aacgttgcgc
     5881 ttgcgccagc ggaacggggg tccaaaaaag cccatgaatc gcacagatcg cattcgcacg
     5941 gctctcccgg gatttgaggt gccggcccaa ttgctccaat tcatagaaac tgcgtccccg
     6001 gaagatcttt ttcgcgtaaa tctggtcaca ctgagcacaa aaatcaatgt tcccctggac
     6061 acgcttcttt ccgcgttttt gaaaatggtg gaggcaggat tgttcaatct atcctgggaa
     6121 taccactgca ctcactgcaa tgcgatcccg gactttaagc acagtttcag cgaactgaaa
     6181 gcagaatcct actgcccact gtgcgacctg ggatttcgca acactctaga caaaaacgtc
     6241 gaagtcacat tcacagctca taattcgttc gtcgaaatcc cgtcggaaat accggaacgc
     6301 atcaaggcgg aagcgatcgc tgcagtgaaa gaaaaacgct atcagatgcc ggagcatttt
     6361 ttgtccggtc ttcaatgcct ggtgaatgaa acatttcacc agctcttcgg agaacaggtg
     6421 ctatcctcag aggaaagcct ggccgtagaa acgatcactc ttctcttcac ggacataaag
     6481 ggctccactc aaatgtattc agatcttggc gacacgaagt cctacgaaat cgtgcgagag
     6541 catttcaaaa tccttttcca ggcgattgtt caaaatggcg gagtcgtagt aaaaacaatc
     6601 ggcgacgctg tgatgggaag ttttctcaag ccagtgaatg caattcgcgc agccgtccag
     6661 gtgcatgacg atttcaacaa gcgagagtgg acctccatcg gctaccttca gatcaaaatg
     6721 ggaattcaca ccggtcacgc cattgtagtc aacctgaacg acactgtaga ctactttggg
     6781 aacactgtga acctggctgc gcgtgtgcag gcatccgtaa agaatcacga catccgtttc
     6841 acaaaaagag tgctcgagga ttcagaagtt gtttcgtatc tcaaagagag aaaggcaatc
     6901 ttgcacaaga aatttgaaat gtttaaagga atcaatgatc cagtcgaagt ctatcgcttc
     6961 acgccgggcg ctccttgatc aattgcgcgc caattcaata gattcaagag cgcgaaccat
     7021 ttcatctgca gcaatcgcag acgtactgta cgcaaactct atgtttaatc gtctgcgaac
     7081 tgcttctgca gtctctctat cataaattcg cagaatcaag cgggcagacg ggtcgagtga
     7141 ccgcgcatgc aggcctatct ccaggttcgt gagatcatca ttgatcacgg aaaacagagc
     7201 aacagcatgc agcaacccgg ccatttccag atttcgaagc aacgtcgcat ctccgtacag
     7261 tatcttgatc ccgcgggcgc gaaatgtatt caagaatcgg ttgtgctcat tgctctctat
     7321 gacaacgatt tgaaaacccc ggcggcgcaa ctccagagct atctgatatc ccagtctacc
     7381 cagtccgcaa agaatcacat ggcctttgag acgatgtgtt ttcctcccca gcagcacctg
     7441 gcttctcctt tccatgagct tgtctactac cagtgcgaag atgattgaaa agaaagaaac
     7501 agaagtcagc attgtacaga tcacgaagag ttttgcttca tcagacgagt agcgaagatg
     7561 aatgtccccg aacccggtgg tagtgatcac agtggtgatg aagtaaaacg catcaatcca
     7621 gcgaagattt tctgaatagc gaaagaaaat cgcgccagct gtatagagaa gcagaaacgc
     7681 cgaaagcaaa acggtcagga gaacgttgga acgaagccag ttgaaaatgc cactgtcagg
     7741 ttcgccctcc tccggtttct gagccatcgc tggcagtgga gcctgtcgca cctgagtcac
     7801 ggcatccgcg aaagtggaag cggcgagtct tgccggattc atgataaata aattcttgca
     7861 attgatttga aaatgcggcg ctatcttctc attgaacaac gccaggaaaa taggctcatc
     7921 tctctcgaaa ctcatggccg caagggcgaa ttcaatattc cttctatctt cgctatccac
     7981 gacatagata gcgcgtgcgc gttcagctcc tgcgtgtttg agctgggttt tgatgctttg
     8041 aagacgggcg actgaataat ccggggagct ttctattcct tccgcgctca agtgacacac
     8101 ggagtaagac ttcttttcca gcacctgtct gatgcgattc gccaggtgac cggatccaat
     8161 tacaatgacc tcttccatca tccgcctcga atcagcaggt tgtctatcaa ccgaacttct
     8221 ccgcagtgca tcgcggctgc gatgacaaaa tcgccttcaa tttcctgcaa tacctcgagg
     8281 gtctcaggat ctaccacctg agtatattcc actctattgt ctggccccgt cagcattaca
     8341 tcctgggcga tctctgccag atccgccggg ttcgcgttac cattgttaaa cgctttttcc
     8401 gcaatgcgaa atccgcgaat catcaaggct gcttgctcgc ggtgttctgg tttcaaattc
     8461 ttgttgcgag aagacatggc aagaccgtct gcttcgcgaa tcgtctcgca acctatgata
     8521 tcaatcggga aagaaaggtc ttccaccatt ctctggatca cgcgaaactg ctggtagtcc
     8581 ttcttcccga aatatgcttt gtgaggcaaa aacagatgaa agagcttcgc aacaatcaac
     8641 aatacgcctt caaaatgacc cgggcgaaac ggtccgcaca gattctttgt taacgctggc
     8701 atggccattt gaagagcagg acccgcaccc agcatttccg atgcagtcgg aacaaaaact
     8761 atatcagcgc ctgcctcttc gcaaatcgct cgatcgtgat cttcgctgac cgggtacgtt
     8821 tcgaaatcac gcgcattgtt aaactgcagg ggattgacaa agatattcac acatacctgt
     8881 tcactctcac gtcgagcatc tcggatcaaa gaagcgtggc cttcgtgtaa atatcccata
     8941 gtcgggacga ggctgcgcac tttgtctctt acagaaagtg tttcgcgaag ttctgccagt
     9001 gaacggatta ttttcatcaa accacctttt gcaattgaat caatctcgcg ttggcttccc
     9061 gaactctgcg cgacattgtc ttaaaaagag caaaagaaat tctggggcga gcgatcagca
     9121 ggcgttgaaa atcagatcga ttgatcacaa gtagacgagt attcctcact gcgaccaccg
     9181 aggcggatct gggttcctga tccaggatcg ccatttcacc gaagcaatcc ctttcctgta
     9241 aaatgtcaat cgtgagctct tttcctttct gtcctttgaa gattctcacg ctgccggata
     9301 tgataatata cagcgattct ccgggatctc cttcgcgaaa gatattggct cgggccctaa
     9361 atttttgctc gtgcgtgatc tcattgatcc agtgtaagtc caccccgtcc acttcagaaa
     9421 aaagcggaac gctgcgaagg aaaagaaatc tctccatgcg tgtgtccatt ttcttgcgct
     9481 ccctttctaa ttgcttatga ttgcgttcct tctgtactgc caggcgaaat gtttttgatt
     9541 tcaacttacg cagtgcaatg cttgcgttga tgcgaaccat ctcgtccggt gcgtcaagct
     9601 gactgaacag ctcaggctca agcatcttca tgttgagcaa tcccgcggca tgaaccgcac
     9661 acgatgtgat ccacggcgga tgattcttcc atagaatgtg ccggagaacc tcatccactg
     9721 tgcctcgaga tgcctgggca ggatcgtctt cgagtacgcg aaccagaacg cctgcctctg
     9781 tacaacttcc ttcaagagct tccagcgctt ctgcccttcc acgcgcgtcg ccaaattgca
     9841 gatctgcggt gagtgcgcga attgttctag gatctccaat gagttccaga aacttcaacg
     9901 ccacctcaag tctctggcgt gcaaatctct gcactgcgtc tagaatcaag agcacggctg
     9961 taggatcaga agccttctgc aaaatggcaa cgttctgcct gtctttctct atattttcca
    10021 cgatcgaaaa atagcacgcc cgaatgcgat ccttcacctc acgcgtaaaa tactcatccg
    10081 cacgagatac atcctgtcct gtttcgacga gaaacttcat tcgaatgttt gtgagagctt
    10141 ccgccgccgc gttttccaca agaatcggct ggcgaacaat cagtttcgca agcacaggta
    10201 tgcttcgcag gctggagatt tcgccaatgc aagcaacgat gcttattttt gcttctgcct
    10261 ctgtttcaat ggccaattgc gcatggagtg cagacaaagc agattcgcca tactgcaaaa
    10321 gaccttcctt tgcggacagg tacacatcgt cttcgcgcaa gtaagcggcc aacaaacgaa
    10381 gtgcttccct ggtcttgagc atccccatcg cacgaactgc ttcgtttcgc acgtcagcag
    10441 tggagtgctc catcaaacga accaggcttt tggctttcgc gggcagaggc acttcccgga
    10501 ggatacgggc tgcctgaaag cgatccggct cttcgcggga tcgcaataaa cgatccaggc
    10561 cttttgttcc gactgtacgc tggcctccgg gccgtgtcag ctgagcgatc acggcttctg
    10621 ccgcgacatt tgcgtgagta tctttgatca gccgtgccat gcttcgcaca tcttcgcggc
    10681 tgcctacaag tgaaagtgcc cgaacggccg ccgcgcgcac aggtccatca gaatctgaga
    10741 cttccgttcg aatggcggag cgtgtttcag agaatccggc tcgtcccaga tacccgagca
    10801 aggctgtttt caagcgcggt gtagtgtgag gaagaacggc gctgaggcga agagcagtgg
    10861 ccagatccgg atgatcgagc agctcaagag cgaataacgc catatcttcg tcgttggagg
    10921 agagtgcggc cagcagaatt tccctggttt ccagatcccg atttgtactc aagagagaga
    10981 aggcgttgcg ctgaaggtcg atgttacgcg aattcaagtt tgcaacgagt gcccgcaagt
    11041 agtctttctt gatacgaagt gcaaggccaa tccacacaag gcacatcgcg agagtgagcg
    11101 aagcaaatac gatgggtgga gcatgaaatc gtccaagtac aagaagcaaa ataccagcca
    11161 cagcagtggc catgggctcc atcgcaccgt tcaaaagagc acgagctctc gcacgattct
    11221 catccggcaa tgaactgaac aaaaccgcaa tggcagaatc atgaacagac ccaaagagag
    11281 tctcatccga aaattttcct gcaatgccaa gaatggagcg cggctcataa acttttccgg
    11341 gcacgacggg catcatactt ctagcacaaa gccccactgc acttgctgcg actactcccg
    11401 ggtaaagaat cgaagtgcca cccactccca gcgtttgaag tagtcttccg gtcacgaaga
    11461 actgaagtat caatcctgca agagaagaga gcccgttaaa aacaccaaga aagccagcga
    11521 gagaatcctg ctctttgtac acttcatcta aagcatgaaa gaattgataa tcaaccagcc
    11581 acgctgtgat ccacattgga aaggcgatca tcgccagtgt gcgaaccagc ggaatctgaa
    11641 acagcgatct ccagttctcg gccacagttt cgccgctgtc gtccgtgcct gcgagaatga
    11701 ctccgctctt tcgcgcagct gctgcagaaa ggcgaagtgg tggaatcgca agaatcacca
    11761 tgcccgccca gccgagtagc agattgttac taccaaacgc atttacgaac atgggagtta
    11821 ctgaacttcc aacgatcatg cccaccagac cggctgcacc tacgatcggg aacatgcgct
    11881 tcccttcgcg cggatcaaaa accttgtttg cgtaggtcca gaagtgcatg agcatcaaaa
    11941 tgttgtacac ttccgcaagt gaccagaccg cgtatggcat tacaacattg ccggatgaga
    12001 tcagtagcct gagaacaacg atggcgagaa cgaaagcagc acacagtaaa cccatgaatc
    12061 tactgagaga caatcgatgc acgacgcgcg aatacacggc cgcaatcccg gcaaccagga
    12121 tgccattgag aagatagacg atcggtagat tgtccgctcc cgcattcttg atgaaaaatg
    12181 catctcgcgc agctcgaccg aatgcaagag ctgccatgat tgcgaaacac aaaagcatca
    12241 tgctgcgagc acgaggccat tctttctcag taatgccaga agctcgcgaa agaaatttca
    12301 tgcacccagc acccgaatgt tctccgcaag cggatctgcc gtaccctgaa gcaagcttcc
    12361 ctgttcttta agcacgcgaa cgtaatctat aatatcctga gtgattcttt ctcctggaca
    12421 aataatggga atgccaggtg ggtaggacat caacatctct ccgctgattt gtccagccga
    12481 tccagataga ggcatgaact tcttgggcga atagaaagcc tggcgcggag aagaaattac
    12541 gtctggcatc agaggcggag gcacagttgg ccttgaatgc tttcgattgt tcccgagcac
    12601 gattgtcttc agacttccga ccagatgcag tatatcttcc tcgcgcgttc cgattgtaat
    12661 gagagcaaga acattgttca tgtccgcaag ctcaacctga atcgaaaagc gatctcgcag
    12721 caatctctcc atctcaaaac cggtgaaccc ggctgaagca caatgaatga gcaacttact
    12781 ttcgtcgtag ttgaagacag aactatccgg cctgcaaatt tcctttccgg gcgaatagag
    12841 accttcaatc aaattgatct ctgttcgtgc gaaacgagcc agctgaagca cgcgttcaaa
    12901 ctgaccgggc gcatcctgtg caagagatct tcgcgcagta tccagagaaa ccatcagcaa
    12961 ataagacgca cttgtggatc gaatcaaatc gatcgactct ttgagaatgt catgcggcac
    13021 tcgttcacct ttggaaagga gaatcgcgct ttgcgtgaga gatccccccg ttttatgcat
    13081 gctgactgca ctcatgtctg cacccacgct catggcattt gcaggaaaat ccggatgaaa
    13141 agaaagatgc gtgccgtgcg cctcgtctac gagagctgca gcccgatgat cgtgcgctgc
    13201 gcgaaccatg ccttcaatgt ccgtcgcaaa accgtagtaa gacggattta ccacaaacaa
    13261 cgcgcaaacg aatggatttt tttccagagt cgcgcgaacg ctctgaaccg ttggacctgt
    13321 ggccacacca agatgagcgt caatctccac gggaacgtaa ataggaaggc cgccagataa
    13381 gatcaaacct gtgatcgcgg aacgatgaca atttcgcgga agtacaattt gctcacctgg
    13441 cccaaccgca gaaagcatca tcgaatgaat tccctgagtg tttccgttta caagaaagtg
    13501 agcgtgatcc gcgccgaata aatgagccgc aagtctctgt gcttcgtcaa tgacccccac
    13561 cgggttgcac gcgtcgtcga gatcatgcat tgcattcagg tcgaacgaaa gagtggtttg
    13621 cccgaacaga ctcaggagat cgcgtgcgcc cactccgtgc ttgtggccag gcacgtgaaa
    13681 tggatgcact ccttccgcat ggtgtgcgat caacgcgtcg agaagaggag ccctgttttg
    13741 gtctagaaac ataggtcgaa ccagtcggta caagatcggc acgcggagag tcaaaatcgt
    13801 ctgaacacgc gtagaactgc agttcggaat tgaattttga gcctcgcgct ggccgatact
    13861 gagacgtgtc ctcagaaatc actttagact acaatgagtt ctcgcctcaa gactacttga
    13921 acgagtatta cacagaagtt tcagacgaaa acgaaagcct gcttcagttc ttccacgata
    13981 cctattcaaa gctcgggcca gtagacagca tgttagaagt cggcggcggt ccgaccattt
    14041 atcagatcat cagcgcgagc agagttgcca aacacataga cttttcagag tatctgcccg
    14101 cgaatcttgc catggtccgc gcctggctag accaaaaacc aggatcattc aactgggaca
    14161 tctacttcaa acgcgttctt gaaatcgaag gaagacctgc ttcgcgcgaa aacatagacg
    14221 cactcgcagg tacggttcgc ggcgcagtga gacggttgac ccaatacgac gtgtttcgcg
    14281 agcctccact ggtggaaggc gaaccgttat acgacataat cgcgtccaat ttttgcattg
    14341 aaggcatcca gacagaccgg caagtttttg aaagacagtt tggtcgtatg acatcctatc
    14401 tcaggccagg cggtctcctg gctctgtgca tggtgaagaa tgcgacatat tatcgcgtag
    14461 gcaaaagacg atttccagtc gtaccagtaa atgaaacgat tatggaaaac gtgttgaaga
    14521 atgcgggcct tactctgact catatccagt cgatcaaagc ggtggatcca gatcagggtt
    14581 acgaaggaat gctgatgatc cgtgcgacga ggggttaaga cgtgagattt ccattcgcag
    14641 taaaacttgc actttcgatt tcactgctcg ctgtttctgt tacgagcttg agcgttgtgt
    14701 ttctatacac ggacacacgc agtatggtga ttcgactcat ggccggacgc ctcaaagacg
    14761 tgggaagaac tgcggcttac atgcttgaat cgcaggaccg cgaaggcatg aaaaaaatgg
    14821 ccacggttgt tcgcagcaaa atcaaaatca cagcggaact cagaaaaaca atcgcggacg
    14881 cgaaagtggg cgatacaatt ccaagccctc tcactcagga agaaatagac aagatcatcc
    14941 aatctccgga ctacctggaa atggttcagt tccttcgc
//
LOCUS       DAOQJL010000015        14943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1908_length_14943_cov_4.808033, whole genome shotgun sequence.
ACCESSION   DAOQJL010000015 DAOQJL010000000
VERSION     DAOQJL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_14943_cov_4.808033"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLG78_01225"
     CDS             <1..655
                     /locus_tag="PLG78_01225"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNE21754.1"
                     /translation="KDSTMEKLADKGNGNYAYIDNFSEARKVLVEQAGGTLVTVAKDV
                     KIQVEFNPAQVSAYRLIGYENRLLRNEDFNNDQKDAGDIGAGHSVTALYEIVRVGKPI
                     QTPGVDPLRYQKPGTAVTNLNGELLQVKLRYKQPDSDTSTLLSQTLSSESPGSTENLR
                     WASGVAMFGMVLRESPYKGNATIESAYSLASKSTGNDPGGYRSEMLRLLRAAEALKR"
     gene            complement(708..2846)
                     /locus_tag="PLG78_01230"
     CDS             complement(708..2846)
                     /locus_tag="PLG78_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE21755.1"
                     /translation="MRNIGRLIPLLILALTIGCDKKILIGVSLPLSGPNAETGKEMLQ
                     AVQLHVDSINEKGGIGGKKVGLVIKDDEDDPAKVKKAAEELAGNSNVVGVIGHLNSPT
                     VIAAAEVYDKTDLVAITPVSTNTDLSTKTKNIFMLNRDGVYQGQFMVDYLKGAMGKSH
                     PIVFVQESRYGLRHWKGFEERASELSITPVKVIRFKNEDLGTEAFAANLTQDPTADSI
                     ILLTDDNADVGLKLLTIIDNSKVAPEDTVMLAPESYLNPKYTTKEAERYTDTLYVCTP
                     FLWEIANQTASAMREAYEAKYKVQPGLGGAMAFDAAMLLTTAIAEKGTDRKLVHEYLL
                     EQKRKDLQAKRKDLLVGATAELVFGEGRFADRDLFVAKVEHGRYKVAFTQLATVKEPY
                     VIAQLPERIANGSVIDLAGTPYHVVDVIFMGLDILKLNSVNTKDMRFEVEFFMWYKWA
                     SKAVDPSQIDAVNGAGDLKTTLLKERTKKGVTYRALRCKGTFNTQMDFSQFPFDRQGL
                     EMSFVNKVKHSAHLFIVPDTRHMTHDPLKGLPLDWTFIQKKNYTGLLRYDSTFGDPDY
                     RMGAGYKSRILFSTIGFRLDVQRVPTGFAFTMFVPLVVILVASFFVISAPIEELKGRL
                     PLGLTALLTVVLSRMALASQTPSVAYIMRSDYFYLTAFLYVMGLIAVLSGVIWLMAKK
                     NDKGARLLHKFFVMVVSPLVAVVLLLQFIL"
     gene            2938..3741
                     /locus_tag="PLG78_01235"
     CDS             2938..3741
                     /locus_tag="PLG78_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE21756.1"
                     /translation="MRLAFCFAFLISSSLLADESLHPGPAFVFGTQVNVREKGATTAA
                     VKFMLPIGAKVTIETATQEKVIQGARSDFWYQIKTADGRNGFVFGSLLTALGEELGDG
                     RLLLVGWAENAKDSFEFRIVQKGSLVSSRAFALLGYSQDPAAANYCHTMELHRNLFGQ
                     GNHAISLLSFAGSEMDAGFSNGAYAFILRGNTIDLVSQGQGGADAPAYADYTFSFRPG
                     IIEEKFEEVGDIQDGIEIGEGYTRQFKLVAGKIAASEKQIYKIRRPVPQ"
     gene            complement(3742..5172)
                     /gene="lpdA"
                     /locus_tag="PLG78_01240"
     CDS             complement(3742..5172)
                     /gene="lpdA"
                     /locus_tag="PLG78_01240"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNE21757.1"
                     /translation="MQCEMLVLGGGPGGYSAAFRAADLGLNTIIVERYATLGGVCLNV
                     GCIPSKALLHVAAVIEEASHIQVAGVQFAKPQVDLDKLKAHKNGVVGKLTNGLSGMAK
                     ARKVEVVRGYGSFISANHLEVEITQGTAQDKTGTKKVIEFKQCIIAAGSAAVHLPFIP
                     IDPRVVDSTGALELRFVPGKMLVIGGGIIGLEMATVYSTLGSRIDIVEMLGAIMAGPD
                     RDLVKAWEKQNMHRFDQVMLNTKTVSVEAKPDGLWVKFEGENAPKEPVRYDMILQSAG
                     RSPNGKKIAAEKAGVQVNERGFIPVDLQMRTNVSHIFAIGDIVGSPMLAHKAVHEGHV
                     AAEVAAGQKSAFDAKVIPGVAYTHPEVAWVGVTEDQAKAEGRAVQTAKFPWAASGRAI
                     ANAAEYGFTKLIFDAETHRLIGGAIVGPSAGDMIGEVCLAIEMGCDSVDIGKTIHPHP
                     TLGETVGLTAEVAHGSCTDLMPVRKK"
     gene            complement(5347..5565)
                     /gene="aceF"
                     /locus_tag="PLG78_01245"
                     /pseudo
     CDS             complement(5347..5565)
                     /gene="aceF"
                     /locus_tag="PLG78_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="E2 component of pyruvate dehydrogenase multienzyme
                     complex; in Escherichia coli AceF contains three
                     N-terminal lipoyl domains; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
     gene            complement(5582..6916)
                     /locus_tag="PLG78_01250"
     CDS             complement(5582..6916)
                     /locus_tag="PLG78_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNE21758.1"
                     /translation="MIEIKVPDIGNYHDIPVIDVCVKPGDTIAVDAALVTLESDKATM
                     DVPSSTAGTVREIKVKLGDRVSEGTVVAIIEASDSASQALREGSSASATASSATTASA
                     SAAASATSGSAQSASSAGASASTANASSPSAGPRATQSPGAPFTLSGKAHASPSVRFF
                     ARELGVDLSKVHATGPKDRILKEDVMAYVKGVMTSGGMGGGLDLLPWPKVDFTKFGPI
                     ETKPLSKIKKISGQNLSRNWVMIPHVTYHEEADITELEEFRVATNKENAATGVKVTVL
                     AFLIKASAMALKKHPEFNSSIDGDNIVFKHYFHIGFAADTPAGLVVPVIRNVDQKSVT
                     EIAAETSVLAKKAREGKLTPAEMSGACFTISSLGGIGGTSFSPIINAPEVAILGVSKS
                     AMKPVWNGKEFIPRLMLPLSLAADHRIIDGALATHFNVLIASLLSDVRRLVL"
     gene            complement(6936..9611)
                     /gene="aceE"
                     /locus_tag="PLG78_01255"
     CDS             complement(6936..9611)
                     /gene="aceE"
                     /locus_tag="PLG78_01255"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNE21759.1"
                     /translation="MAEPQLHSANTDIDPNESQEWREALDGVIAREGPDRAHYLIGQM
                     IAQAREAGIDIPYSATTEYINTVPVEKQPKYPGNAAMEIKIHNYIRWNAMAMVVRANK
                     STNVGGHIASFASAAALYDVGFSWFWRAPSENHGGDLVFYQGHSVPGIYARAHLLGRL
                     TDEQMDNFRQEVDGKGISSYPHPWLMPDFWQFPTVSMGLGPLQAIYAARFMRYMESRG
                     FIPAQDRKVWAFLGDGETDEVESLGAIGMAGREDLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELESEFRGAGWNVIKLVWGTHWDALFARDKKGVLKKRMMEVVDGEYQTFKAKDGA
                     YVRKYFFNTPELEALIADWTDEDVWALNRGGHDIFKIFAAYKSAVEHRGQPTVILAKT
                     IKGFGMGQAGEAMNISHQQKKLDHEAIKRFRDRFSLPVSDEDLDHLPYLKFAQGSPEL
                     AYMQERRANLGGHLPTRRQKAPALEIPPLSDFDALLKATGEGREISTTMAIVRLLNIL
                     LKDKKIGRHIVPIIPDESRTFGMEGLFRQIGIWNQEGQKYTPEDKDQLMFYKEAKDGQ
                     VLEEGINEAGAMADWIAAGTAYSTHGVQMIPFYICYSMFGFQRTMDLCWAAGDSRTRG
                     FLIGGTAGRTTLNGEGLQHEDGHSLVLAGLVPNCIAYDPTFSFEIAVIFQDAMRRMYA
                     EQEDIYYYITVMNENYEHPDMPAGTERDIIAGMYLFKKGAEAPGKPRVQLLGSGTIFR
                     EVIAAAELLRNDWGVESDLWGCPGFNQLAREGQDALRYNMLHPGETPRISHVEKCLSG
                     TTGPVIAATDYVKQYADQIRAFVPRKYVVLGTDGFGRSDTRTKLRHFFEVDRHWVTIS
                     ALSALADEGKIDRKKVAEAISKYGINPGKPGPLTA"
     gene            complement(9674..12829)
                     /locus_tag="PLG78_01260"
     CDS             complement(9674..12829)
                     /locus_tag="PLG78_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21760.1"
                     /translation="MFFRREILLLLCFTSAPAFARMPVALENLRWSAREGFVESYRQG
                     LPSGTGTRSVDGFPISMFTLFPCPTDGFTHHFTISAPFELEPDDFSFQEPAGIFLRGL
                     GDNWELYVNGTLVHSEMNLNGGGQIAIRKTSMGKLIPFEKAILHKGTNTIVLHLAGTA
                     SIASAVNPAVPGLFFSKDYTMDRLSSLQHRYSERIDLALLAVYLFFGLYHLVLFLGRR
                     KDKYNLYFALFTISLAVYGFSRSYAIYDSIEDSSLVTRVENASVALLIPLLIVFLSDY
                     FYQKWIHPRLNWIICGFNFLAAALFCVVPFDFIAHVGWIWKTAAIPVILIYAPFIVVE
                     SVIKKARDAKILATALAIFVLVAIFDLVRDALKLFSMEPLFQYAFFGLVLSIAGILAH
                     RFVTVFNESDRLNQELAEKNETLRELDRVKDEFIGTISHELRTPLNGIVGLAEALTSG
                     RANDADIETGRTLGLIVDSGRRLSRLVGDLIELVRLKNRDLKVNPLPIRIADSVDVVL
                     SLIAPLVESRPITISSEIKPETPAVFADPDRLQQILFNLIWNAAKFTDKGRVTIRARP
                     SIKQPGFVTIEVEDTGIGIPAEKQRVIFEAFRQADSSIARPYSGAGLGLSITRYLVEL
                     HGGSISVTSTPGKGSIFCFTLPASTGLPLAAGSTATIEPIRYTTIRAPQTKKDRAKSS
                     ILAVDDEPINLEVLRTQLSLEGYDVTTAENGERALQMIEEDPPDLVVLDIMMPRISGY
                     QVCEEIRKHYSIHELPVLLLTARNQLTDLLRGFDSGANDYLTKPFEARELSARVRTLL
                     AVRHASQEKESHTALAKELAIARDIQRAVLPRTAPHVRGLTLATRFRPRDSVGGDFFD
                     FYAGEKTLDLIMADVSGHGVPAALIAAMIKIAFSHQRGVTDPAALLFGLNRALWGNLD
                     RHFVTAIALSIDLERRLLCLASAGHPPLLIHRAPNTSFYSARGRVIGLVEEAYYSKLE
                     VQLEPGDMLVVYTDGLTEARAPSGDLYGEDRLSAFIETHSHLSSDDLAEALEQDVVQF
                     AGETLDDDLALIVIQIDPKVN"
     gene            12885..13643
                     /locus_tag="PLG78_01265"
     CDS             12885..13643
                     /locus_tag="PLG78_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNE21761.1"
                     /translation="MTLFRALLILLLAVQSTYSQPPTASSIYGPVSREDYLLGRFRVD
                     QHPAFIKLSSTAIPTRGREQFLRKEAAEALGRLLGDLKRDHPQAEFWVVSSTRNYYDQ
                     KGIWESKWNGGTLVEGKALNKSIQDPAKRAQKILEFSSMPGTSRHHWGTDFDMNVLTN
                     VYYDSGNGKIVYDWFKKNASKYGFCQPYTAGRKKGYYEERWHWSYLPISRLLLADWKT
                     LFDHHTEKLKGFEGFAASGHLAPTYVESINPDCQ"
     gene            complement(13647..14099)
                     /locus_tag="PLG78_01270"
     CDS             complement(13647..14099)
                     /locus_tag="PLG78_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21762.1"
                     /translation="MSESRCDPSGRIGTCRIEKPGTVIFVHHYPPALKENLQPTCEKF
                     GGTLYAPGEDFINDMRPVEKRLERCNDLIDLLRKLYEGAPASPQESSCSEYGKMIQLR
                     LTEAPGLDAEQRSAVTMIGRTLCTSKQNGTMSFAAFEAEFRKKRACPK"
     gene            complement(14715..>14943)
                     /locus_tag="PLG78_01275"
     CDS             complement(14715..>14943)
                     /locus_tag="PLG78_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21763.1"
                     /translation="PLYDPSVCALVFSGYWVDVPGLHNERFPPFSRKEMEIEDWIDQA
                     YLWKWEELPPDVLKHNKALSEYIAKHLKSPP"
BASE COUNT         3463 a         3807 c         3768 g         3905 t
ORIGIN      
        1 gaaagattct accatggaga aactggcgga caaaggaaac ggaaactatg cttacattga
       61 caatttttcc gaagcacgca aggtacttgt cgagcaggcg ggtggaactc tggtcacggt
      121 cgcaaaagac gtgaagattc aagtggagtt caatccagcg caggtgagcg cctatcgttt
      181 gattggatat gaaaatagat tgttgcgtaa tgaagacttc aacaatgatc agaaagatgc
      241 aggcgacatt ggtgccgggc attccgtgac ggccctctac gaaatagtgc gagtgggtaa
      301 gccaattcaa actccgggag tagatccact tcgttatcag aagccgggaa cggctgtgac
      361 caatcttaac ggagaacttc ttcaggtaaa gctgcgttac aaacaacctg attctgatac
      421 aagcactctg ctgagtcaga ccctttcctc tgaatcgccg ggatccactg agaatcttcg
      481 ctgggcttca ggtgttgcta tgtttggtat ggtgcttcgc gaatcacctt acaagggaaa
      541 tgcgacgatc gagagcgcat actctcttgc cagcaagtcg acaggaaacg atccaggtgg
      601 ctacagatca gaaatgctcc gcctattgcg cgcggctgag gcattgaaac gctgattgtg
      661 gacggaactt cgcggatagc tccgcgaagt tcttctacct gagagaatta gagaatgaac
      721 tgaagtagca gaaccacagc caccagagga gatactacca ttacaaagaa cttgtggagc
      781 agtcttgcac ctttgtcgtt cttctttgcc atgagccaga taaccccgga aaggacggcg
      841 atcaatccca tcacatagag aaaagcagtc agatagaaat agtccgatcg cataatgtag
      901 gctacggatg gcgtttgcga agcaagtgcc attctggaca gtacaacggt gagaagtgca
      961 gtgagtccca gaggaagacg tcctttcagt tcttctatcg gtgcggagat tacaaaaaag
     1021 gatgcaacga gaatcacgac cagaggaaca aacatcgtga aggcgaagcc ggttggcact
     1081 cgctgcacat ccaggcgaaa ccctatagta gaaaatagaa tccgactttt gtatccagcg
     1141 cccatcctgt aatccgggtc cccgaaagtg gaatcatatc tcaaaaggcc agtgtagttc
     1201 ttcttctgta tgaacgtcca atcgagcggc aggcctttca gaggatcgtg agtcatgtgt
     1261 cttgtatcag ggacaatgaa tagatgtgcg gaatgcttta ctttgtttac gaaagacatt
     1321 tccagtccct gtctatcgaa tggaaactga ctgaagtcca tttgagtgtt gaaagtgcct
     1381 ttgcatctga gcgcgcgata agtgacacct ttctttgtcc tttctttgag aagcgtagtt
     1441 tttagatcac ccgcgccgtt gactgcgtca atctgagaag gatccactgc tttgctggcc
     1501 cacttgtacc acatgaagaa ctctacttcg aagcgcatat ccttcgtgtt gacggagttg
     1561 agcttcaaaa tgtccagacc catgaaaatt acgtctacaa catgatacgg tgttcctgcc
     1621 agatcaatga cggagccatt ggcgatccgc tccggcaact gtgcgatcac gtaaggctct
     1681 ttgactgtgg ccagttgagt gaacgcaact ttgtatcttc cgtgctccac tttcgctaca
     1741 aaaagatctc tatccgcaaa acggccctca ccaaacacca gttctgcggt tgctcctacg
     1801 agcagatctt ttctttttgc ctgcagatcc tttctctttt gttccagaag gtattcgtga
     1861 actagcttac gatctgttcc tttttctgca atcgccgtgg tcaaaagcat ggcggcgtca
     1921 aaagccatgg ctccacccag gcccggctgc actttgtatt ttgcctcgta ggcttcgcgc
     1981 atggcagatg cagtttggtt tgcaatttcc cataggaagg gagtgcaaac gtaaagagtg
     2041 tccgtataac gttccgcttc tttggttgtg tactttggat tcaagtaact ttcaggcgca
     2101 agcatcacag tgtcttctgg agccactttg ctattgtcta tgattgtgag aagcttgagg
     2161 cccacgtccg cattgtcgtc tgtaagaaga atgatggaat ctgcagttgg atcctgggtg
     2221 agattggccg caaatgcttc tgtacccaga tcttcgttct taaagcgaat gactttgacg
     2281 ggcgtaatgg aaagttcgct tgcgcgctct tcaaatcctt tccaatgcct gagcccgtag
     2341 cgcgattcct gtacaaatac gattggatgc gatttgccca tggctccttt cagatagtcc
     2401 accatgaatt gtccctggta aacaccatcg cgattgagca tgaatatgtt ctttgtttta
     2461 gtacttagat ccgtgtttgt gctaactggt gtgatggcca ccagatccgt cttgtcgtaa
     2521 acttccgccg ctgcgatgac agtgggcgaa ttcaaatggc caattacccc aactacattc
     2581 gagtttcccg caagttcttc cgcggccttc ttcaccttcg caggatcgtc ttcgtcgtcc
     2641 ttgatgacca atccaacttt ctttccgcca attccgcctt tttcgttgat agaatccacg
     2701 tgcagctgta ctgcctggag catttctttt ccagtctctg catttggtcc gctgagaggc
     2761 aatgaaacgc cgatgagtat ttttttatcg catccaatgg tgagtgcaag gatgaggagt
     2821 ggaatgagcc ggccgatatt tcgcatggtt ttttgtgaga aacggtagtg tggcagaacg
     2881 tcaagcgcaa ccatattcct tcttgttttt ttagcatccc tggacaggat gagagccatg
     2941 agactggcgt tctgttttgc tttcttgata tcctcttctc ttctggcgga cgaaagcctt
     3001 catcccggtc ctgcatttgt gtttggaacc caggtcaacg tgcgagagaa aggcgcaacg
     3061 actgctgccg ttaagttcat gttgccgatt ggtgcgaagg tgaccattga gactgcaacg
     3121 caggaaaagg tgatccaggg cgccagaagt gatttctggt atcaaatcaa aactgcagac
     3181 gggcgaaacg gttttgtatt tggatcgctt ctgaccgcgc ttggcgaaga actcggcgac
     3241 ggtcgcctgc ttcttgttgg ctgggcggag aatgcgaagg actcgtttga gtttcgcatt
     3301 gtgcaaaaag gaagtctggt ttcatcacga gcgttcgcgc tccttggtta ctctcaagat
     3361 ccagctgccg ccaattactg tcataccatg gagttgcatc gcaatctatt cggtcagggc
     3421 aatcatgcga tttctctctt gagttttgca ggctctgaga tggacgctgg attctcaaac
     3481 ggggcttacg cattcattct gcgcggaaat acaatcgacc tggtttcgca gggtcaggga
     3541 ggagcggatg ctcctgcgta tgcggactat acgttttctt tccgtcctgg aatcattgaa
     3601 gaaaaatttg aagaggtggg agacatacag gatggaatcg aaatcgggga aggatacacg
     3661 cgtcagttca agctcgtggc gggaaagatt gcggcttctg aaaagcagat ctacaaaatt
     3721 cgcagacccg ttccccagta actatttctt tctcactggc attagatccg tgcagcttcc
     3781 gtgagccact tccgcagtta gtccaacagt ttctcccagg gttggatgcg gatggatggt
     3841 tttgccaatg tctactgaat cgcatcccat ttcgatggca aggcacactt ctccaatcat
     3901 atctcctgcg gatggcccga cgatcgctcc gccgatcagg cggtgagttt ctgcatcaaa
     3961 gatgagcttt gtaaatccat actccgccgc gtttgcaatc gcgcgtccgg atgcagccca
     4021 cgggaatttc gcagtctgca ccgcacgccc ttctgccttt gcctggtctt ccgtgacacc
     4081 cacccacgct acttcaggat gagtgtatgc cacgcctgga atcactttcg catcaaacgc
     4141 gctcttctga ccggccgcca cctccgctgc cacatggcct tcgtgcactg ctttgtgagc
     4201 gagcatgggg gagccaacaa tgtcgccgat tgcgaatatg tgcgaaacat ttgtccgcat
     4261 ttgtagatct acaggaatga atcctctttc gtttacctga acgcctgctt tctctgcagc
     4321 aatcttctta ccgttaggcg atcttccagc gctctggagg atcatgtcgt atcgcaccgg
     4381 ctccttcggg gcattttctc cttcgaattt cacccagagt ccgtctggct ttgcttctac
     4441 tgaaacagtt ttcgtgttca acatcacctg atcaaagcga tgcatgtttt gcttttccca
     4501 cgccttgacc agatcgcggt ccggacctgc catgatcgct ccgagcattt ctacaatgtc
     4561 aatgcgagag cccagggtag agtacacagt cgccatttcg aggccgatga ttccaccacc
     4621 tatgaccagc atcttgcctg gaacaaatct gagctcgagt gctcccgttg aatccacgac
     4681 gcgcgggtcg atcggaataa atgggaggtg aaccgcagcg ctcccggctg ctatgatgca
     4741 ctgtttgaat tcaatgactt tcttggttcc cgttttgtcc tgggctgtgc cttgcgtgat
     4801 ttctacttcc agatggttgg cacttataaa gcttccgtaa cctctcacta cttccacttt
     4861 gcgagccttt gccataccgc tgagcccgtt tgtgagtttg cccacgactc cgttcttatg
     4921 tgctttgagt ttatccagat ccacctgagg tttagcgaat tgtactccgg cgacctgaat
     4981 atgagatgct tcttctatga cggctgcgac gtgcaaaagt gctttggacg gaatgcatcc
     5041 tacgttcaag catactccac ccagtgtcgc ataacgttcg actatgattg tgttcagccc
     5101 cagatctgct gcgcgaaagg cagctgaata gccacccggt ccgcccccaa gaaccagcat
     5161 ttcgcactgc atgtccaccg tgccgctgta ggtggaagct gtggctgcgg aagaaggtgc
     5221 cgggccttgc gtgggtgcag gtgctgtaga agaggaggcg gaagaggcca gagtgggatt
     5281 cgcagatgag gaagagggcg cgggcgatgt ggcgctggag gtgctcgatg cctcaatgac
     5341 cgcaaccgca tgtccttctg aaacccgatc gcccactttt actttaattt ctttaacaac
     5401 tccggccaca ggactcggga tgtccatggt tgctttttca gattctacgg tgagcagagc
     5461 gtcttccgct tttaccgtat ctcccgcctt cacgcataca tctattacgg ggatatcttt
     5521 gtagtttcct atgtccggga cttttactgt aattaattcg ctcatggtat ccctcgtagc
     5581 cttacagtac caggcgtcgc acatcgctta gaagcgatgc gatgagcacg ttgaaatgag
     5641 tggcaagcgc tccatctatg attcgatgat ctgcggccag agagagagga agcatgaggc
     5701 gcggaatgaa ttcctttcca ttccacaccg gcttcatggc ggatttgctt acgcccagga
     5761 tcgccacttc tggcgcattg atgataggcg aaaacgaagt accaccgatt ccgcccagac
     5821 tcgaaatggt gaagcaagcc cccgacattt ctgcaggagt gagttttccc tctcgagctt
     5881 tcttcgcaag aaccgaagtc tctgccgcga tttccgtaac cgatttttga tctacgttgc
     5941 gaatcacagg cacgaccaga cccgcaggcg tgtctgcggc gaatcctatg tgaaagtagt
     6001 gtttgaatac aatgttgtct ccgtcgatgg agctgttgaa ttcaggatgt ttcttgagag
     6061 ccatcgcact tgctttgatt agaaatgcaa gtaccgtgac cttcacgcct gttgcagcgt
     6121 tttccttatt ggttgcgacg cgaaattctt ccagttccgt gatgtccgct tcctcgtggt
     6181 aggtgacgtg tggaatcatc acccagttgc gcgaaaggtt ctgtcccgat atttttttga
     6241 tttttgaaag tggtttggtt tcgatcggac caaacttcgt aaagtctact tttggccatg
     6301 gcagaaggtc cagtccacca cccatgccgc ctgacgtcat cacgcctttt acataggcca
     6361 tgacatcttc ttttagaatt ctatcctttg gtccggttgc atgaactttg ctcagatcta
     6421 ctcccagttc acgtgcgaag aatcgcacag atggacttgc atgagctttt ccactcagtg
     6481 tgaatggcgc tccgggggat tgagttgctc gcggccccgc agaaggcgaa gaagcattcg
     6541 cagtactggc agaagctccg gcagaagagg cagactgtgc agatcctgat gttgcggagg
     6601 cggcggcaga agcagatgca gttgtagcag acgatgctgt cgcagaagcg ctcgagcctt
     6661 ccctgagcgc ctgcgacgcc gagtcggatg cctcgatgat cgcaacgact gttccctccg
     6721 aaactctgtc tcccagcttc actttgattt cgcgcaccgt gcctgctgta gaagaaggaa
     6781 cgtccatcgt ggccttgtct gattccagag tgaccagagc cgcatccact gcgattgtat
     6841 ctcccggttt cacgcagaca tcaatgacag ggatgtcgtg atagtttcca atgtctggaa
     6901 ctttgatttc aatcatttga cccatgcgat gcctctcaag ctgttagcgg gccaggtttg
     6961 cctggattga ttccgtattt tgaaattgct tccgcaactt tcttgcgatc gatctttcct
     7021 tcatccgcaa gcgcggagag agcagaaatc gtaacccagt gccgatccac ttcaaagaaa
     7081 tggcgcaact ttgtgcgagt atctgatctt ccaaagccat ccgttccaag cacaacatac
     7141 ttgcgcggaa cgaatgctct gatttgatcc gcatattgtt ttacatagtc cgtggctgca
     7201 atcacagggc ctgtcgtgcc agagaggcat ttctccacat gagaaatgcg aggtgtttct
     7261 ccaggatgaa gcatgttgta cctcaaagca tcctggcctt cgcgtgcgag ctgattgaat
     7321 ccaggacaac cccagagatc cgattccact ccccagtcat ttcgcagcaa ttcagccgca
     7381 gcgatgactt cgcggaagat ggtgccagaa ccaagcagct gtactcttgg ttttcctggc
     7441 gcttccgcac ctttcttgaa tagatacatc cccgcgatga tgtcgcgttc tgttccggca
     7501 ggcatgtcag gatgttcgta gttttcgttc atcactgtga tgtagtagta gatgtcttct
     7561 tgctccgcat acattcttcg cattgcatcc tggaagatga ctgcgatctc aaaagagaaa
     7621 gttggatcgt acgcaatgca atttggaacg agtcctgcca gcaccagaga atgtccgtct
     7681 tcgtgctgca atccctctcc gttcaaagtg gttctacccg cagtcccgcc gatcagaaat
     7741 ccgcgtgtgc gcgaatcccc tgcagcccag catagatcca tcgtgcgctg gaatccaaac
     7801 atcgaataac agatgtaaaa cggaatcatt tgcacgccat gagtggagta tgcggtgcct
     7861 gcggcgatcc agtccgccat cgcacctgct tcattgattc cttcttctag aacctggccg
     7921 tcttttgctt ctttatagaa catcaactga tctttgtctt ctggtgtgta cttctgcccc
     7981 tcttgattcc agattccaat ctgtctgaac aaaccttcca ttccaaaggt gcgcgattcg
     8041 tccggaatga tggggacaat gtgcctgccg atcttcttat cttttagaag aatgttcagc
     8101 aatcgtacga tggccatggt agttgaaatt tctctacctt ctccggtggc tttgagcaat
     8161 gcgtcaaaat cagagagcgg aggaatttca agtgccggcg ccttctgtct tctggtagga
     8221 agatgtccgc ccaggttcgc acgcctttcc tgcatgtagg cgagctctgg actgccctgt
     8281 gcgaatttca aatacggcag atgatccagg tcttcatcac tgacaggaag gctgaatcga
     8341 tcgcggaatc gtttgattgc ttcatgatcc agtttctttt gctggtgtga aatgttcatg
     8401 gcttctccag cctgtcccat tccaaagcct ttgattgttt ttgctagaat gactgtgggt
     8461 tgccccctgt gttctactgc ggatttgtac gcagcaaaga ttttaaagat gtcatgtccg
     8521 cctcgattga gtgcccagac atcctcgtct gtccaatctg cgatgagtgc ttccagttct
     8581 ggagtattaa agaaatactt acgaacgtat gcgccgtctt ttgctttgaa agtctggtat
     8641 tcgccgtcca cgacttccat cattcttttc ttcagcacgc ctttcttatc gcgtgcgaag
     8701 agcgcatccc agtgcgttcc ccataccagt ttgattacgt tccagcccgc tccgcgaaat
     8761 tcactttcca gttcctggat gatctttccg tttccgcgaa ccggtccgtc cagtctctgt
     8821 agattgcagt tgattacaaa gatgaggttg tccaggtctt ctcgtcctgc catgccaatg
     8881 gcaccgagcg attctacttc atctgtttct ccgtctccta gaaacgccca tacctttcta
     8941 tcctgtgctg gaatgaatcc gcgactttcc atatagcgca tgaatcgcgc agcatagatt
     9001 gcctgcagag gaccgagccc catggatacg gttgggaatt gccagaagtc aggcatcagc
     9061 catggatgcg gataagacga gattcctttt ccgtccactt cctggcggaa gttgtccatt
     9121 tgttcgtccg tcaggcgacc aagtaaatga gctcgtgcat aaattcctgg tacagagtgc
     9181 ccttgataga agacgagatc tccgccgtgg ttttcagagg gagcgcgcca gaaccaggaa
     9241 aacccaacat cgtagagagc tgctgcggat gcgaaagaag caatgtgtcc gccaacgtta
     9301 gtcgacttat tggcgcgcac aaccatggcc attgcgttcc agcgaatgta gttgtgaatc
     9361 ttgatttcca tagcagcgtt gccgggatac ttgggctgct tttctacggg cacggtgttg
     9421 atgtattccg tagtcgcgct gtaaggtatg tcgattccgg cttcgcgcgc ctgtgcgatc
     9481 atctggccaa tgagatagtg agccctgtct ggaccttctc ttgcaatgac accgtccagt
     9541 gcttctcgcc actcttgcga ttcgtttgga tctatatctg tatttgctga atgcagctgg
     9601 ggctctgcca tttatgcctc ctccccggaa caacgcgtgg cgcgtcagac tgtgggagcc
     9661 tgcctggcgg acgtcaattg acttttggat cgatttgaat tacgatgagg gccagatcgt
     9721 cgtccagtgt ttcccccgca aactgaacca cgtcctgttc cagggcttcc gcaaggtcgt
     9781 cgctggaaag gtggctgtgg gtttcaatga aagcgctgag gcgatcttct ccataaagat
     9841 cgccagacgg agcacgggct tctgtcaggc catctgtata tacgaccagc atgtctccag
     9901 gctcaagctg gacttctaac ttgctgtaat aggcctcctc aaccagaccg atgactcttc
     9961 ctctggcgga atagaaactg gtgttaggag ccctgtggat cagtagaggt ggatgaccgg
    10021 cagacgccag acaaagcagc cttctctcca ggtcaatgct cagagcaatg gcagttacaa
    10081 aatgacgatc cagatttccc caaagcgcgc gattcaatcc aaacaaaagg gccgccggat
    10141 ctgtgactcc tctctggtgt gagaacgcaa tcttgatcat ggcagcaatg agcgccgctg
    10201 gaactccatg tccggacaca tctgccatga tgagatcaag agtcttttca cctgcgtaga
    10261 aatcaaaaaa gtctccgcct acactgtctc tggggcggaa gcgagtggcc agtgtaagac
    10321 cacgcacgtg aggcgccgtt cgcgggagca ctgcgcgctg aatatcgcga gcaatggcca
    10381 gttcttttgc aagcgcagtg tgtgattctt tttcctggga agcatgcctc acggcaagta
    10441 gagtgcgcac tctagcactc agttcgcgcg cctcaaaagg ttttgtcagg tagtcgtttg
    10501 cgcccgagtc aaaaccgcga agcaagtccg tcagctgatt tcgtgcggtc aaaagaagta
    10561 caggaagctc atggatcgag taatgtttgc gaatctcttc gcacacttga tatcctgaaa
    10621 tacgcggcat catgatatcc agaaccacca ggtccggcgg atcttcttct atcatttgaa
    10681 gagcccgctc gccattttcc gcggtggtta catcatatcc ttcgaggctc agctgcgtgc
    10741 gaagaacttc gagattgata ggttcatcat ctacggccag aatgcttgat ttcgcgcggt
    10801 ctttctttgt ctgaggagct cgaatggtcg tataacggat tggttcaatg gtggctgtgc
    10861 tacccgctgc gagtggcaat cctgtgctgg ccggaagagt gaaacagaag atggatcctt
    10921 tccctggtgt agatgttacg gagatagaac caccgtgcaa ttccaccaga tagcgagtga
    10981 tgctcagacc cagacctgct cccgaatagg gacgcgcaat gctggaatct gcctggcgaa
    11041 acgcttcgaa aattacgcgc tgtttttctg cgggaatccc aattcccgtg tcttccactt
    11101 cgatggttac aaaacctggt tgtttgatgc tcggtctggc gcgaatcgtc actctccctt
    11161 tgtccgtgaa tttcgccgcg ttccagatca ggttaaaaag aatttgttgc aggcgatctg
    11221 gatccgcgaa tactgcgggc gtttccggtt tgatttcaga tgaaattgtg attggtctgc
    11281 tttctacgag aggtgctatg agcgacagca ccacgtctac agaatccgcg atgcgaatcg
    11341 gcaaggggtt caccttcaga tcgcggtttt tcaggcgtac gagttcaatg aggtctccca
    11401 ccagccgaga aagtctgcga ccgctgtcca cgatcaatcc aagagtacga cctgtttcta
    11461 tatcagcatc attggctctg ccagaagtga gcgcctccgc aagacctaca atgccgttta
    11521 acggtgtgcg aagttcgtga gaaatcgttc caatgaattc atcttttacc cgatccagtt
    11581 ctctcagcgt ttcgtttttt tctgccagct cctggttcag tcgatctgat tcattgaata
    11641 cggtgacgaa tctatgagcg agaattcctg caatggatag aaccagacca aagaatgcgt
    11701 attggaaaag aggctccata gagaagagct tcagcgcgtc tctgaccaga tcgaatatgg
    11761 ccacgagcac aaagattgcc agcgcagtgg ccaggatttt tgcatccctg gctttcttga
    11821 tcacggattc tacgacaatg aatggcgcgt agatgagaat gactggaatg gcagctgttt
    11881 tccagatcca tcctacgtgt gcgatgaaat caaatgggac tacgcagaac aaggcggctg
    11941 caagaaaatt gaatccgcaa atgatccaat tgagtctggg gtgaatccat ttctggtaga
    12001 agtaatcgct taaaaaaaca atcaaaagag ggatgagaag ggccacgctt gcattttcga
    12061 cgcgagttac gagagaggaa tcttctatag aatcgtaaat ggcgtaggag cgagagaatc
    12121 cgtacactgc aaggctgatt gtaaaaagag cgaagtacag attgtactta tcctttcttc
    12181 tacccaggaa aagaaccaga tgatacaatc cgaagaatag atacactgca aggagagcca
    12241 gatcgattcg ttccgaataa cggtgctgca aagaggacag gcggtccatc gtgtaatctt
    12301 tgctaaagaa caaaccagga acggcgggat tgaccgcgga tgcgatggac gccgttcctg
    12361 cgagatggag cacaatcgta tttgttcctt tgtgcaggat tgctttttcg aatgggatga
    12421 gtttgcccat cgacgttttt cgaatggcga tttgtccgcc cccattcaga ttcatctccg
    12481 agtgcaccag ggttccgttc acgtagagtt cccagttgtc tccgagcccg cgcagaaaga
    12541 tcccggcagg ttcttgaaac gagaagtcgt ctggctctag ctcaaagggg gcggaaattg
    12601 tgaaatgatg tgtgaatccg tccgttggac agggaaaaag agtgaacatg ctgatcggga
    12661 agccgtctac tgaacgtgtc cccgttccag acggtagacc ttgcctgtat gattccacaa
    12721 atccttctcg tgcggaccag cgaaggtttt cgagtgcaac cggcatgcgc gcgaaagctg
    12781 gagcggatgt gaagcaaagc agaaggagga tttcgcggcg aaagaacatc gaagatgtga
    12841 ggatttacga atatgcttca atttagacga gaaaaaaatt gcccatgaca ttgtttcgcg
    12901 ctctgctgat tctccttctg gcagtacaat cgacatactc ccaaccgccc accgcctcat
    12961 cgatctacgg accagtgtca cgagaagact atctgctcgg tcgctttcgc gtggaccagc
    13021 atcctgcatt catcaaactt tcgtctacgg ccattcccac tcgaggtcgc gaacaatttc
    13081 ttagaaaaga agctgcagaa gcgctcggtc gccttcttgg cgatttgaaa cgcgatcatc
    13141 cccaggctga attctgggtt gtgagctcta cccggaacta ttacgatcaa aagggaatct
    13201 gggaaagcaa atggaacgga gggactctgg tagaaggaaa agcactcaat aagtccattc
    13261 aggatcctgc gaaacgcgcc cagaagatac tggagttttc atccatgccc ggaacatctc
    13321 gccatcactg gggaactgat tttgacatga acgttctcac caatgtttac tatgattctg
    13381 gaaacggaaa gatcgtctat gactggttca agaagaacgc gtctaaatac ggattctgtc
    13441 aaccatacac tgcaggaagg aagaaaggat actacgagga aagatggcac tggtcgtatc
    13501 ttcctatttc ccgcctcctc cttgcagact ggaagacgtt attcgaccat catactgaaa
    13561 aactaaaagg tttcgaaggc tttgctgcgt ccggtcacct cgcgcccaca tacgtggagt
    13621 ccatcaatcc ggattgtcaa taggagtcat ttcgggcagg cccttttctt tctaaattca
    13681 gcttcgaaag ccgcaaagga catggttcca ttttgtttgg aagtacacag tgtgcggcct
    13741 atcatggtga ctgcgcttcg ctgctctgcg tcgaggcccg gcgcttctgt cagacgcaat
    13801 tgaatcatct ttccgtattc gctgcagctc gattcctgcg gtgaagcagg tgctccttcg
    13861 tagagtttgc gcaataaatc gatcaaatcg ttgcaacgtt ccagtctttt ttccacgggg
    13921 cgcatgtcgt ttatgaaatc ttctcccggt gcatacagag tccctccaaa cttttcgcac
    13981 gttggttgca ggttttcttt gagagcgggg ggatagtgat gaacaaaaat gacagtgccc
    14041 ggtttttcta ttctgcacgt gccgattctg ccggagggat cgcagcgcga ttctgacact
    14101 tttcctccaa tcgcgcagta gtcttctgca acactgcgtg tggcttccct tcgcatccgg
    14161 tgaacacctt ctgatggcac tcttgtcccc tcccggctta tccgggagtc cgtcttcttt
    14221 tggagatccg gcgctttctt tccaggagga tacttccttt gtttcctcaa gcaacttgcc
    14281 aggatcaggc tgctttcttg gagaggggcc cagtgtcagc gtataaccag agttaggtgc
    14341 gtggtatttt cctctctgcg cgcaaaactc aggaagcccg gatccgttcc gctgcggaga
    14401 atagtaatga accagaaaag ggccccgttc tgattgcatt tcgcagctcc ccactcgatt
    14461 gcgaagcgcg cacggaatgt aattcacagt catgtcttcc ccgcagtaga acccgggacg
    14521 ccctgattct gcaggaaatt ctacgcacac tccgaacacg ttcggagtgc ccctcccatc
    14581 catggaacag ctcatgagat tttcgcgtgc agggagggat gcaaaagaag cgagtgcgga
    14641 cgcgaacaca gccgaaagtg caaagacgct gactgcgcgg gtacgcacgt gccgattgcc
    14701 cttcagctca tggatcacgg cggactcttc agatgttttg caatgtattc tgaaagcgct
    14761 ttgttgtgct taagaacgtc aggcggcaat tcctcccatt tccacaaata agcctgatcg
    14821 atccagtctt cgatttccat ttctttacgc gaaaacggcg gaaatctctc attgtggagg
    14881 ccgggaacgt ctacccagta tccactgaac acaagtgcac atacggaagg atcatagagc
    14941 ggg
//
LOCUS       DAOQJL010000016        14794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1937_length_14794_cov_4.990298, whole genome shotgun sequence.
ACCESSION   DAOQJL010000016 DAOQJL010000000
VERSION     DAOQJL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1937_length_14794_cov_4.990298"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLG78_01280"
     CDS             <1..399
                     /locus_tag="PLG78_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21764.1"
                     /translation="AYYAGFFVRMNLLSPQDYGGATQAKKEAEAARLRAKEARATEVA
                     QARQLTSQEAILRQNIKLLDDSLIMNQEQIYVSERLFVSGAINALQMAEILSRRADLI
                     SARYESEAELIRTRAARFILSGKLPSGETK"
     gene            396..3551
                     /locus_tag="PLG78_01285"
     CDS             396..3551
                     /locus_tag="PLG78_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE21765.1"
                     /translation="MKSAQPVKEPNPGFAGSLARNFLHSHLTPIIAVASLVLGIASVY
                     LTPKEEEPQISVPMIDIRTNLPGYEAHEVEQKVTEVIEKAVWGLEGVEYVYSASSPHS
                     SLVTVRFKVGEPVEPSLVKVHHKIMEIRSSLPQEASQPLVKSYSIDDVPFLVLTFQSS
                     TRDDFSLRSVVSPLARDLAGTPDISRVDIYGGRKQSIRVVVDPARSSAAGVSILDVAH
                     ALKANHEGVLSGKNWGKDNVVSVEAGGLLSTAQDVARTGVGQRAGLVVRVGDVATVTD
                     GPEERSRLSYVMDHTGKSNAVSLSFAKRKGTNAAELSVDVMKRAERFASTLPSDIHMT
                     VMRNYGQSAKDKSSELIEHLIIATISVGVLIAITMGLRASAVVSLTIPVTLALTLGIY
                     YFMGYTLNRVTLFALIFSIGILVDDAIVVVENVERHLRESPHLSAAQATLRAVAEVGN
                     PTILATFTVIAAILPMMFVRGLMGPYMKPIPVGASFAMILSLLVAFIVTPWAAVRLLK
                     KHTHSETKASKLDALYDKVMRTLLSKKKSALVFGGVTVFLLLASMGLIASKAVKVKML
                     PFDNKNEFQVLLDYPASTPLEKSSAESVLLAQELLKHPDVERVQIFAGESAPYSFSGL
                     VKHTFLRAQDYKTDLQVVLKDKDHRKKKSHEIIEEIRPRIADFARAHNALSKVLEIPP
                     GPPVMATLVAEVYGPDNLSREKAARTVQQIFAAEKSVVDLDYSWRPERDRRVYNFNVN
                     GGALLGINASDASQASLLTFSETPIVSLAESNSPEIKTVDLSLAQSARSGASPFRGQT
                     IATREAGTANLDQVFGNPQIKTFETLHRKNLKPVSYVVSELSGAEEAPVYGIMSLGPK
                     IPFQLQTVDVPWNMDQPVVKWDGEWYITYEVFRDLGLAFAAVLVLIYVLVTGWFKSYA
                     VPLVIMAPIPISLIGILPGHAAFGAYFTATSMIGFIAGAGIIVRNSIILVDFIEHQIE
                     EGAELKEAVIQAGITRFRPMLLTAAAVVVGTAVILFDPIFQGLALSLMFGEVAATLIS
                     RIAVPVLYHWFIGKSRAAIRA"
     gene            complement(3541..3825)
                     /locus_tag="PLG78_01290"
     CDS             complement(3541..3825)
                     /locus_tag="PLG78_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNE21766.1"
                     /translation="MKQVASTDELQQLVNRLSRAQGQLEGIKKRLVAGAQPDCKQTIH
                     QLKAAHRAITEFAEAYVLHYFEQCVTESDQPPDRKVEKNLREAISAAFTL"
     gene            complement(3888..4388)
                     /locus_tag="PLG78_01295"
     CDS             complement(3888..4388)
                     /locus_tag="PLG78_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE21767.1"
                     /translation="MDPLLDEKVVLTLGRAFTEHMKNTLFVSAEKEAYGPSKNEGICA
                     ESCSGIGFTGELNGMMYLCMDGYTKMKLLPRIAERYQIDAGQRGMADSVLLEFSNQIA
                     SNILSELRDGGYHLELQPPESLNHKIVPIDLKRNRQYILIFFLRDRRMKRYLGRATLV
                     LTLEKY"
     gene            complement(4388..5512)
                     /locus_tag="PLG78_01300"
     CDS             complement(4388..5512)
                     /locus_tag="PLG78_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21768.1"
                     /translation="MKFQLPDLSPLAAIQALAERVGAVSFAIQTSWGPFFVNLDRIVI
                     ISIFLILAGAALLILWAVMRSYAPAPETEPEIESIATAEPVEAPAEIPELQTISRQDA
                     LRIRPEFFMRKVSDELSRHLSFASSIDELLRAFLIHASSVCDGILTVFLRNEQGDMVP
                     RLRSQGKIVLGGDFSRVLSETNVGHTGFEILASGKPVMSEDGRQAAFSIDSPTEIAGF
                     LLLESEHASDLKVLSRLHDMCYQFSLFMYEKIHQIENQAHSGTSGRLQLESALRVLSQ
                     ASAAFSLTICEFSEGKPDISGDLIFCEGNRLYVLGGVESERTLDARFASWIHRCGPAS
                     IGIAVSMGQEEPAKIFDRALRALENAKAAGLNHYRILQAA"
     gene            complement(5615..6325)
                     /locus_tag="PLG78_01305"
     CDS             complement(5615..6325)
                     /locus_tag="PLG78_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21769.1"
                     /translation="MHRAILVLTIACCSCNSVKHECNPLPPAPHSVAVLGDSRIFDPT
                     PKNFAGFANYLNQDATFKYFRNTTFTSEDSDPRTSTRTPGSDIYSSVGNTTAYALHNL
                     PRVLAGPHDAMLIHLGVNSTGDPIGASRDLAAIAESARAKSWQVYIVTVGPWRGYPTW
                     TQLYQDGTDGINRWIRDQGVRYSVIDVQPVVEDREHLQYLRADYTWDKLHYTSIAHSA
                     IAADWISHIKTKGCGWDL"
     gene            complement(6331..7467)
                     /locus_tag="PLG78_01310"
     CDS             complement(6331..7467)
                     /locus_tag="PLG78_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE21770.1"
                     /translation="MEFLKNPFLVRPFIILGIVLGLDKLLLLQAVREYTTSYMHIEDH
                     FYDTREDLLNHLKEHYTARKAAGEKLGLIFGTSRSGEFDSAEISKIAPKTYTYNFSAP
                     QAGHSYHAYWLQRILEQNIRPDFVLLESDPITLSPNANRFPIKHSLDISFLLKHIDFD
                     SDASPWRNGGGFSTEHFERFLLSRMFAAYRFPVDLRAAARNRESHIMPVGPGIQMVTG
                     LEIRRAMLAQMKEASIRNLGGIPNPHALELPENLMNRDAETMASLHLPRPYHTGRAQT
                     RLFREMLEGLARESIPTVVFWPPVVSRFREIMRETGAQDMARSGTTEIIERLGKDYPK
                     AKLRFVEADSKGMQCRIFFDSIHIAGKCYPELTKLLLKEIQSDL"
     gene            complement(7477..8913)
                     /locus_tag="PLG78_01315"
     CDS             complement(7477..8913)
                     /locus_tag="PLG78_01315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE21771.1"
                     /translation="MRFTTLYFLFFFALVYVVFWIIPRRFRPYLIFVASLGFYAAWSW
                     QFAVHLLLVLLVNYLALIRLLGRPRRSTLALIVLLNVVNLVFFKYFYFILGTASQAVG
                     VIGPERLDAWLEHNLGVSHIVLPLAISFYTFQLIALQVDAYRGKIPERPSIIEYLMFS
                     LYFPHFVAGPIMRHSDFFYQLRNLKPDETRMLDGLLLVGAGLVKKVVIADNVDFVLKP
                     VFANPGGYDWSTNLMAALGFAARIYCDFSGYTDIARGSSKMLGLDIPENFKAPFLSAS
                     VGEFWNRWHVTLMMWLRDYVYIPLGGSRRSPARNYANLILTMALSGLWHGASFTFLLW
                     GLWNGVFVSIEKLLGVRTEFEPGFIRDGFVSSVARLAFRFGGMVYAYLVFALGLILFN
                     SQSLQSAMNMYGQIFFLRSGAEWPHTEYVFNIVAITLLFNIVQSRKQFHIPVGWKSYP
                     VLGALGLVVLLLLGSFPPGTTDFIYFQF"
     gene            9082..9417
                     /locus_tag="PLG78_01320"
     CDS             9082..9417
                     /locus_tag="PLG78_01320"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE21772.1"
                     /translation="MIKASSAHFSEQVRDIARFGRLLSHPARPRILKLLAKREFMTCG
                     EIVEALPFAQATVSRHLRELTSGGLIRLDVEGLRSFYSLDNRAASRMQTCYQEFFRMI
                     ASKETGGSR"
     gene            complement(9470..10258)
                     /locus_tag="PLG78_01325"
     CDS             complement(9470..10258)
                     /locus_tag="PLG78_01325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE21773.1"
                     /translation="MKRTLLALLLVFAFVACQKNHVRGEEVTYKFGDQTLKGLLAYAP
                     EQTGKRPAVLVVHEWWGNNDYSRKRAMQLAELGYVALAIDMYGDGKIAANPDEAGKMA
                     GGVMKDFEKGKGRFAAALDFVKQQPQVDPTRIAAIGYCFGGGVVLNMARQGADLKGVV
                     SFHGGLDPVKPAKKGEVKARILVAHGGADSFVPMEKVDAIKKEMSEAGADLTVTVYPG
                     ATHVFTNPDADKYAKEFKMPIAYNAEADKKSWEEMANFLSSVLK"
     gene            10382..10873
                     /locus_tag="PLG78_01330"
     CDS             10382..10873
                     /locus_tag="PLG78_01330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE21774.1"
                     /translation="MPATESKELVQGQDSTGSEIFHKMFDSFLEETAAAGVQIPPNSI
                     LESGAVFLEYESRTRLVSEFPAQEKHANPLGMLQGGIIGALIDDTMGPLSFAAVRGPT
                     TTLNLSINYLRSVKCPDRVRVEARVTGRGRRVLHLEADVFDSRGRLVAKSTSTVLSLK
                     EGG"
     gene            10873..11379
                     /locus_tag="PLG78_01335"
     CDS             10873..11379
                     /locus_tag="PLG78_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNE21775.1"
                     /translation="MVRRSAKEIPDSRDLRLAWLSCLALNLKRTARLVSAAYDDAVRP
                     SGLRSTQFSILATVAVNPYVSIAQLADWVDADRTTVQRSIGIMQRNLWITVEKAEQGN
                     LRRLKLSAKGQKKLAEAYKLWEQAQKQMVDILGTEASRRLLHDLSRARKKTREKLVTQ
                     SESVASIS"
     gene            11472..11912
                     /locus_tag="PLG78_01340"
     CDS             11472..11912
                     /locus_tag="PLG78_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNE21776.1"
                     /translation="MNPAPHLKPEERVVLFDGVCVLCTWWTRFLTRYDVNHKFLLCSI
                     QSPEGLDILAWAGIPVDDVNTMLYVSNNTAYTRSDAFIEVMGQLGLPWSLARILKIVP
                     RFFRNACYNFIAHNRYRFFGRSDVCMMPTPENQSRFLKSQTAKS"
     gene            12007..12375
                     /locus_tag="PLG78_01345"
     CDS             12007..12375
                     /locus_tag="PLG78_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21777.1"
                     /translation="MRTLLLALTILAIVHCGPNKKDSYYCDITSQSLDTCDGKTIKVT
                     ANHSDRIQQHPVISPDKQSYWDVNGRDWIFVSKNAIDCKTGIVVTGRLDAKVGPCDPT
                     AQNHNQYCGTAVYVDNWKCQ"
     gene            12427..>14794
                     /locus_tag="PLG78_01350"
     CDS             12427..>14794
                     /locus_tag="PLG78_01350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE21778.1"
                     /translation="MEQPGLDQFLPAASSWFEQTLGQPTRIQERSWEALKSGAPALIS
                     APTGSGKTLAAFFSILDDLFRKSLAGNLAQESTVLYISPLKALSNDIEKNLRIPLDGI
                     ARELAARGTPLSSPIEVMVRTGDTTPAERARMMKQKPHIIVTTPESFYVMLTSERGRS
                     MLSTVDTLIVDEIHAIVSSRRGSHLALSIERLAALTRKPLQRIGISATVKPLDRVAEF
                     LGAKKEVRILDEGHNRPMDVKMITPDVALDAVMTQEGWAQIHEKLIELMKSARTTLIF
                     VNNRRLCERLARQLSDLIGQDLVGAHHGSLAHDRRRNAEQALKAGTLKVMVATSSLEL
                     GIDIGSVDLVVQIASPRSIHGFLQRIGRAEHHKDGVSRAVLVPLTRDDLVECAALLRA
                     IKQGKLEEIEIPQAPLDILAQQLVAELACRSMTADQAFDLVRSANPYKTLERNKFEQI
                     LEMLSDGYSLRFGRRSRYVFYDRALGTLEARRGARLSAITNGGAIADNFEYEVVLADE
                     GAFLGTVHEDFAIESMAGDVFQLGNQFWKIDRITTGKVLVSHAPNSTPTMPFWIAEAP
                     GRSDELSVAVSNLRSDSQPRMQEGLEGFAEWLEEETGLGGAAATTLFEYLREGVLVLG
                     QMPTRNKIVLERFFDEAGDSHIVLHSPYGTRINRAWGLALRKRFCRKFNFELQAAATD
                     DAVLFSLSKTHSFALGEVFSYLKSEIAEKILVQALLAAPMFEIRWRWNASRALAILRQ
                     YGSRRTPPQIQKSQAQDLVALVFPDQIACAENLAGDREVPDHPLVEQTI"
BASE COUNT         3882 a         3779 c         3673 g         3460 t
ORIGIN      
        1 gcatattacg ccggcttctt tgtgcgaatg aatctgctgt ccccgcagga ttacggaggg
       61 gcaacacagg caaagaaaga agcagaagcg gctcgcctcc gcgcaaaaga agcgcgcgca
      121 actgaagtgg cgcaagcaag acagctcacg tcccaggaag caatactgcg acagaacatc
      181 aaacttctcg atgatagttt gattatgaac caggagcaga tttatgtttc ggagagactc
      241 tttgtttcgg gtgcaatcaa tgcactgcag atggcagaaa ttctttcgcg tcgagccgac
      301 ctaatttcag ccagatacga atctgaagca gaactcatcc gtacacgcgc cgcacgcttc
      361 attctgagcg gcaaacttcc ttccggagaa acaaaatgaa atcagcacag cctgtgaaag
      421 aaccaaatcc tggattcgcc ggatcgctcg cgcgaaactt ccttcactct cacctcacgc
      481 cgatcatcgc cgtggccagc cttgtgcttg gaattgcgtc cgtctacctc acaccaaaag
      541 aagaagaacc gcaaatctcc gtgccgatga tcgacattcg caccaatcta cccgggtatg
      601 aagcgcatga agtagagcaa aaagtaacag aggtgattga aaaggcagtc tggggtctag
      661 aaggagtgga gtatgtttac tccgcgtcca gccctcattc cagtctagtc actgtgcgat
      721 tcaaggttgg tgaacctgta gagccgtcgc ttgtaaaagt gcatcacaag atcatggaga
      781 tccgtagctc actcccacag gaagcatctc aacctcttgt gaaatcttac tccatcgatg
      841 atgttccatt cctggttctc acatttcagt ctagcactcg cgatgacttc tcattgcgat
      901 ctgttgtctc tccgctggcg cgagatctgg ctggcacacc tgacatcagt cgcgttgata
      961 tctatggcgg aagaaagcaa tccatccgcg ttgtagtaga tcctgcacga agttcagcgg
     1021 cgggggtctc catcctcgac gtcgcacacg cgctcaaggc gaatcatgaa ggagttctat
     1081 ctggaaaaaa ctgggggaaa gacaatgttg tttctgttga ggcgggagga ctcctgtcta
     1141 ctgctcagga cgtggctcga accggtgtag gccagcgggc cggcctggtt gtgcgagtcg
     1201 gagacgtggc gacagtcaca gatggcccgg aggaacgatc tcgcctgtcc tacgtcatgg
     1261 accacactgg aaaatcgaac gcagtctctc tctcattcgc gaagagaaaa ggaaccaacg
     1321 cagccgaatt gtccgtagac gtgatgaagc gcgctgaacg attcgcgtcc actcttccat
     1381 cggacatcca tatgaccgtt atgcggaatt acggacaatc agcgaaggac aaatcgagtg
     1441 aactgataga acacttgatc attgcaacca tttccgtggg cgtactgatc gcaattacca
     1501 tgggactcag ggcttccgcc gtggtctcgc tcacaattcc ggtaactctt gctctaacac
     1561 ttggaattta ctatttcatg ggctatacat tgaatcgagt cacgttattc gctcttatct
     1621 tctctattgg tattctggtg gatgacgcca ttgtagtggt cgaaaacgtg gagaggcatc
     1681 tacgggaaag ccctcacctt tctgcggccc aggctaccct gcgcgctgtg gcagaggtgg
     1741 gaaatccaac aattctcgca acattcacgg tcattgcggc gattctgccc atgatgtttg
     1801 ttcgcggtct gatgggaccc tacatgaaac caattccagt tggtgcaagc tttgccatga
     1861 ttctttctct gctcgttgct ttcattgtta ctccatgggc cgccgtgcga cttttgaaaa
     1921 agcatacgca ttctgaaaca aaggcaagca aactggacgc tctttacgac aaagtgatgc
     1981 gaacactgct gtctaaaaag aagagtgcgc ttgtttttgg aggtgtgact gttttcctac
     2041 tgcttgcgag catgggactg attgcaagca aagctgtgaa agtaaagatg ctcccctttg
     2101 ataataagaa tgaattccag gttcttctgg actacccggc gtctactccg ctcgaaaaat
     2161 cttccgcaga gtctgtgttg cttgctcagg aactgctgaa gcatccagat gtggaacgcg
     2221 ttcaaatctt cgccggagaa agtgctccgt attcattctc cggcctggta aagcacacct
     2281 tccttcgcgc gcaggattac aaaacggatc tgcaagtagt tctgaaagac aaagatcatc
     2341 gcaaaaagaa gagccacgaa atcatcgagg aaatccgccc gcgaatcgcc gatttcgcgc
     2401 gcgcgcacaa cgcactatcc aaggtcctcg aaattcctcc tggtccgccg gtgatggcaa
     2461 cgctcgttgc agaagtctac ggtccggaca atctctctcg tgaaaaagca gcacgcaccg
     2521 tgcagcagat ttttgctgcg gagaaatcgg tcgtggatct ggactactcc tggagaccgg
     2581 agagagaccg gcgcgtgtat aacttcaatg taaatggagg agcactcctc ggaattaacg
     2641 caagtgacgc atcccaggcc tctcttctta cgttttctga aacgcccatt gtttcgctcg
     2701 cggaatccaa cagtccggag atcaaaacgg tagatctctc tctcgcgcaa tctgcgcgct
     2761 ccggtgcttc tccgtttcgc ggacagacaa ttgctacaag agaagcggga accgcaaatc
     2821 tagaccaggt gtttggaaat ccacagatca aaacatttga aaccttgcat cgcaagaatc
     2881 tcaaacctgt ttcgtatgtg gtctcagaac tttccggagc ggaagaagct cctgtgtacg
     2941 gaatcatgag cctgggtcca aagattccct tccagcttca gacagtagac gtgccctgga
     3001 acatggacca gcccgttgta aaatgggatg gagaatggta catcacctac gaggtgtttc
     3061 gcgatctggg ccttgccttc gcagctgtgc tcgtcctgat ttatgttctc gtgaccgggt
     3121 ggtttaagag ttatgctgtt ccgcttgtga tcatggcacc gattccaatc agcttgatag
     3181 gaattctgcc gggccacgca gcatttggcg cctacttcac cgcgacttca atgattggat
     3241 tcattgcggg ggccgggatc atcgtgcgaa actcgatcat acttgtggat ttcatagaac
     3301 accaaatcga agaaggagcg gagctgaaag aagcggtcat tcaagcaggc atcacgcgat
     3361 ttcgtcccat gcttttaaca gctgcagctg tggttgtggg aacagccgtg atcctgtttg
     3421 atccaatctt tcaggggctc gcactgagtt tgatgttcgg agaagtggct gcgactttga
     3481 tcagtcgcat tgcggtcccg gtactatacc actggtttat aggaaaatca agagcggcga
     3541 tcagagcgtg aacgccgcgc tgattgcttc gcgcagattt ttttctactt tgcgatccgg
     3601 tggttgatcc gactcagtga cgcactgctc gaagtagtgt agaacatatg cttctgcaaa
     3661 ctccgtgatc gcgcgatgag ctgctttgag ctgatgaatg gtctgcttgc aatccggttg
     3721 ggcgccggct accaggcgtt ttttgatgcc ttccagttgc ccctgcgcgc gactcaatcg
     3781 attgacaagt tgttgcaatt cgtctgtgga tgcgacctgt ttcatataca atacacagta
     3841 aggtatacaa aaggaacgtc aagcgctttt gagaaatgtt gtcgttttca atatttctcg
     3901 agagtcaaaa ccagagtcgc ccgtcccagg tatcgtttca ttcttcgatc tctcaggaag
     3961 aatatgagaa tgtactggcg attgcgtttg agatcaatag gcacaatctt atgattgaga
     4021 ctctctggcg gctgaagttc aagatgataa ccgccatcac gaagttcgct tagaatgtta
     4081 gaagcgatct ggtttgaaaa ttcgagtaga actgaatctg ccatgcctcg ttgtcccgca
     4141 tcgatctggt atctctccgc aatgcgagga agcagcttca tcttcgtata tccgtccata
     4201 caaagataca tcatgccatt gagttcgccg gtaaaaccga ttccgctaca gctctctgcg
     4261 cagattcctt cattcttcga aggcccgtaa gcctcctttt ctgcactcac gaacagagtg
     4321 ttcttcatgt gttcggtaaa tgcgcgtccg agagtcagaa cgaccttctc gtctaacaac
     4381 ggatccatca ggcagcctgg agtatacggt agtggttgag tcctgcggct ttcgcattct
     4441 ccagggcacg cagcgctcgg tcaaaaattt tcgcaggctc ttcctggccc atagaaacag
     4501 caatcccaat actcgcagga ccgcatctgt gaatccagga tgcaaatctg gcgtcaagag
     4561 tacgttccga ttcaactccg ccaagcacat acagacgatt gccttcgcag aaaatcaaat
     4621 caccggatat atcgggcttc ccttcagaaa attcgcagat tgtaagagaa aatgccgcgg
     4681 atgcttgcga aagaacgcga agcgcagatt caagttgaag tcgaccgctt gtcccgctat
     4741 gagcctggtt ttctatttga tggatctttt catacatgaa gagactgaac tgatagcaca
     4801 tgtcatgcag cctggatagt accttaagat cagatgcatg ttcagattcg agaagtaaaa
     4861 acccggcaat ctccgttggt gaatcgattg agaatgccgc ctgacgtcca tcttcagaca
     4921 tcacgggctt gccgcttgcg agaatctcaa atccggtatg acccacgttt gtttcagaaa
     4981 gcacacgcga aaaatcgcca ccgagaacta tcttgccctg gcttctcaaa cgaggaacca
     5041 tatcgccctg ttcgtttcgc agaaaaacag ttagaatgcc atcgcatacg gaactggcgt
     5101 gaatcaagaa agctcgcagc aattcatcaa tgctggacgc gaaggaaagg tgtctggata
     5161 gttcgtctga aacctttctc atgaagaatt caggtcgaat tctcagtgca tcctgtctcg
     5221 aaattgtttg aagctcagga atttcagcag gggcttcgac tggttctgca gtcgcgatcg
     5281 attctatttc tggttctgtt tcaggtgctg gtgcataaga tcgcataacg gcccatagaa
     5341 tcaatagtgc agccccggct agaatcagaa agatagaaat gatgacaatg cggtccaggt
     5401 ttacaaagaa aggaccccag cttgtctgga tggcaaacga aactgcaccc acgcgctccg
     5461 cgagggcttg gatggctgca aggggagaca ggtcgggcaa ctggaacttc actgtttgta
     5521 ttttcggagc gggggcatgc cgatttgaag gcaatcgggg cgaaaggagt gaaaaactga
     5581 gttttccggg ctaaaaccag acctggcgaa cccttcagag gtcccaccca cacccctttg
     5641 tcttgatgtg tgaaatccaa tccgccgcga tcgcgctgtg cgcaatcgat gtatagtgga
     5701 gcttgtccca ggtatagtcc gcgcgcaagt attgaagatg ttccctgtct tccaccacag
     5761 gctgcacatc aatcacagag tatcgcacgc cctgatctcg aatccatcga ttgatgccgt
     5821 ctgttccgtc ctggtaaagc tgagtccacg ttggataccc tctccatggc ccaactgtta
     5881 caatgtatac ctgccaggac tttgcacgcg cagattccgc aatggctgca agatcacgcg
     5941 aagcgccgat tggatccccg gttgaattca ctccaagatg aatgagcatc gcatcgtgag
     6001 gaccggccag aacgcgcgga agattgtgaa gagcatatgc tgttgtgttc cccacactgc
     6061 tgtaaatgtc gctgccggga gttctcgtgc tcgtgcgagg gtctgaatct tcagaagtaa
     6121 aggtagtatt cctaaagtat ttaaaagttg cgtcctgatt caggtaattg gcaaaccccg
     6181 caaaattctt tggagtggga tcaaagatgc gagaatcccc tagaacagca accgagtgcg
     6241 gcgcaggcgg aagaggattg cattcgtgtt taactgagtt gcacgaacaa caagcaatgg
     6301 tcagtaccag gatcgcacgg tgcatttttc ctacaaatca ctctggatct ctttgagcaa
     6361 gagtttcgta agctcggggt agcatttacc cgcaatgtga atagaatcaa aaaagattct
     6421 acactgcatg cctttcgaat ccgcctccac gaaacgcagt ttcgctttcg gatagtcctt
     6481 ccccagccgc tcgatgatct ccgtcgtgcc ggaccgggcc atgtcctgag ctcccgtttc
     6541 tctcataatt tcgcgaaatc ttgagactac cggcggccag aacactacag tcggaatgga
     6601 ttcgcgagcc aacccttcca gcatttcgcg aaacaacctg gtctgcgcac ggccagtatg
     6661 atacggccga ggcaggtgaa gcgaagccat ggtttctgca tccctgttca tgagattctc
     6721 tggaagttca agcgcatgtg gatttgggat tccgcccaga tttcgaatgc tggcctcttt
     6781 catctgagca agcatggctc ttcgtatctc aagacctgtg accatctgga ttcctggtcc
     6841 aaccggcatg atgtgcgatt cgcgattcct ggcagcggca cgcaaatcga ccgggaatct
     6901 gtaggccgca aacatacggc tcaacagaaa tctctcaaaa tgttcggtag aaaatcctcc
     6961 tccgttcctc cacggagacg cgtcagaatc aaagtcaatg tgcttcaaca aaaaagaaat
     7021 atccagagag tgcttgatcg gaaaacgatt cgcgttaggc gacaatgtaa ttggatctga
     7081 ctccagaaga acaaagtccg gacgaatatt ctgctccaga atcctctgca accagtacgc
     7141 atggtaagaa tgaccagcct gaggcgcgct aaaattatac gtataggtct ttggtgctat
     7201 tttcgaaatc tctgcgctat caaattctcc ggatcgtgac gttccaaaaa tcaaacccag
     7261 cttttcgccg gcggcttttc tggcagtata gtgttccttc aagtgattga gtaagtcttc
     7321 gcgcgtgtca tagaagtgat cttcaatgtg catgtaagat gtagtgtatt ctcgcacggc
     7381 ttgcaatagg agaagtttgt ccaatccgag cacaattccc aggatgatga aaggtcgaac
     7441 gaggaaaggg ttcttcagaa attccatgtc gcatcctcaa aattgaaagt agataaagtc
     7501 cgtggttccc ggcggaaaag aacccaacaa tagcaataca accagaccca gagccccaag
     7561 caccggataa ctcttccatc caaccggtat gtgaaactgc ttccgagatt gtactatgtt
     7621 aaacaaaaga gtgatggcga cgatgttgaa cacatactcc gtgtgtggcc attccgcgcc
     7681 actacgcaaa aagaaaatct gtccgtacat gttcatcgca ctttggagac tttgcgaatt
     7741 aaacaggatc aaccccaacg cgaatactaa gtaagcatat accattccgc caaaccggaa
     7801 tgcaagtcgg gcgacgcttg aaacaaatcc atctctgatg aatccgggtt cgaactctgt
     7861 gcgcacgccc agaagcttct caatactcac gaatactcca ttccagagcc cccagagcag
     7921 aaacgtaaaa cttgctccat gccagaggcc tgaaagagcc atggtcaaaa tcaagttcgc
     7981 ataattacgc gcaggcgatc tcctggaccc acccagagga atataaacgt agtctcgcaa
     8041 ccacatcatc agagttacat gccagcgatt ccagaactct cctaccgacg cagacaaaaa
     8101 gggtgcttta aaattttccg gaatatccag gccaagcatc ttgctggaac cacgcgcgat
     8161 atccgtgtag ccagaaaaat cgcagtagat gcgcgcagca aagccaaggg ccgccatcaa
     8221 attcgttgac caatcgtatc cgccaggatt tgcaaacact ggcttcagaa caaaatcaac
     8281 gttatccgca atcactactt tctttaccag acctgcgcca acgagcaaga gaccgtcaag
     8341 cattctggtt tcgtcgggct tgaggtttcg aagttgatag aagaaatccg agtgcctcat
     8401 gatgggcccg gctacgaaat gcgggaaata gagactgaac atcaaatact caatgatgga
     8461 tggcctctca gggatctttc cgcggtaagc atccacctgg agagcgatca gctgaaatgt
     8521 ataaaagctg atggccaatg gcagtacaat gtgagacaca ccaagattgt gttccagcca
     8581 cgcgtccaga cgctccggcc cgatcactcc cactgcttgc gatgctgtcc ctagtatgaa
     8641 atagaaatac ttaaagaaca ccaggtttac aacgttcagt aatacgatca aagccagagt
     8701 ggaacgccgt ggacgaccca ggagccggat caaggccaga tagttcacca gcaatacgag
     8761 cagtagatgg acagcgaatt gccatgacca ggcggcataa aaaccaagag aagcgacgaa
     8821 gattaaatac gggcgaaatc tgcgcggaat gatccagaaa acgacgtaga ccagcgcgaa
     8881 aaagaagaga aaatagagcg tcgtaaatct cattgccgta atcccttaca atcctgaata
     8941 gttgaggaac tatatattct cgataaattc tttccaataa atgcgtttcg cgcaatcagt
     9001 ttttgactgt tctgtcagga aaaaatactt tacgaaagaa attctgattg ttgaaaacaa
     9061 tatatcgcat atagtcgata tgtgattaaa gcatcgtcag ctcatttctc agaacaagtc
     9121 cgcgatattg cacgcttcgg tcggcttttg tcgcaccctg cgagacccag gattttgaag
     9181 cttcttgcga agcgcgaatt catgacatgc ggagagattg tggaagcact tcctttcgcg
     9241 caggcaaccg tctcacgtca cttgagagaa cttacttcgg gtggcttgat tcgcctggat
     9301 gtggaagggt tgcgctcctt ctattccctt gacaatcgtg ccgcgagcag gatgcaaact
     9361 tgctatcagg aattctttcg aatgatcgcg tcaaaggaga caggaggatc caggtaagcg
     9421 cgagattcag gcgcgcgaat gcgcgcctga cgttgaaaat cggagaggat tattttagaa
     9481 cggaggaaag gaaatttgcc atttcttccc aggatttttt gtctgcctct gcgttgtagg
     9541 caattggcat tttaaactct ttcgcgtact tgtccgcatc tggatttgta aacacatgcg
     9601 tcgcacccgg atagaccgta acggtcaaat cggcacctgc ttcgctcatt tccttcttga
     9661 tcgcgtccac tttttccata gggacaaagc tgtccgcgcc gccatgagca acgagtatgc
     9721 gagctttcac ttctcctttt tttgcaggtt tcacaggatc gagcccgcca tgaaagctaa
     9781 cgacaccctt gaggtctgcg ccctggcgag ccatgttcag gacgacacct ccaccaaagc
     9841 aatacccaat cgcagcaatg cgagttgggt ctacctgagg ctgctgtttg acaaaatcga
     9901 gagctgcggc aaatctgcct ttgccttttt caaaatcttt catcacacca ccggccatct
     9961 tcccggcctc gtccggattg gccgcgatct tgccgtctcc atacatatcg atggccagcg
    10021 caacatatcc aagctcagca agctgcatgg cgcgcttgcg agagtaatca ttgttgcccc
    10081 accattcgtg cacaacaagt acggccgggc gttttccggt ttgttccgga gcataggcga
    10141 gcaagccctt caaggtctga tcgccaaact tataagtcac ttcctcacct cgtacgtggt
    10201 ttttctgaca cgcgacgaat gcgaaaacaa gcaacaatgc taagagggtt cttttcatca
    10261 cccttactgg cctctctatg aaattgcgtc aaatccaact cggccgatga aaaaatactg
    10321 gacgggccag ccgacatagt gtatatacat catatagtgc atatgcactc gggagggcat
    10381 tttgcctgct acagaatcaa aggaacttgt ccagggacag gatagcacag gatccgaaat
    10441 ctttcacaag atgtttgact cctttttgga agaaactgca gccgcaggag tccagattcc
    10501 gccgaactcc attcttgaat ccggtgcggt atttcttgag tatgaatcgc gcaccagact
    10561 cgtttctgaa ttccccgctc aggagaaaca cgccaatcct ctgggaatgc tccagggagg
    10621 aattatcggt gctctcatag atgatacgat gggcccgctc agctttgcgg cagtacgcgg
    10681 accaaccact accctcaacc tatccataaa ctatctgcga tcggtaaaat gcccggaccg
    10741 tgtgcgagtg gaggctcgcg taaccggacg tggaagaaga gtgcttcatc tggaagcaga
    10801 tgttttcgat tcgcgcggca gacttgtggc aaaatcgacg tccaccgtac tgtcattgaa
    10861 agaaggcggc tgatggttag aagaagcgcg aaagaaatcc cagacagccg cgacctgagg
    10921 ctcgcctggt tgagctgcct tgcattgaat ttgaaaagaa ctgcgcgact tgtgagcgct
    10981 gcatacgatg atgcggtcag accgtccggg ctcaggtcca ctcagttctc gattcttgca
    11041 accgttgcag tgaacccgta cgtgagcatt gcgcaattgg cagactgggt ggatgcggat
    11101 cgcacgacgg tgcagagaag tataggaatc atgcaaagaa acctgtggat cacagtggag
    11161 aaggcagagc aaggaaacct gcgcagactg aagctatccg cgaaaggaca gaagaaactc
    11221 gcggaagcat acaaactctg ggaacaggct caaaaacaaa tggtggatat cctcggaacg
    11281 gaagcctcga gacgtctgct gcatgacctt tcgcgtgcgc gaaagaaaac cagagaaaag
    11341 ttggtcacgc aatctgaatc cgttgcctcg atttcgtgaa gcatcaaagc tctgaacatc
    11401 cttcactcga atgaaaagtt tttcatggaa aagagttgca ctctcctcat gaaagcaatt
    11461 gattcagaga gatgaatccc gcaccgcatc tgaaaccaga ggagcgcgtc gtgctctttg
    11521 acggagtctg cgtgctatgc acctggtgga ccagatttct gactcgatac gacgtaaatc
    11581 ataagtttct actttgcagc attcagagtc cggaaggact cgatattctt gcctgggctg
    11641 gcattcccgt agatgacgta aataccatgc tttatgtttc caataacaca gcgtatacca
    11701 ggagcgacgc tttcatcgaa gtgatgggtc agcttggcct tccctggtcg ctagcgcgaa
    11761 ttctaaagat cgtcccgcgc ttctttcgca acgcatgtta taacttcatc gcacacaatc
    11821 gttacaggtt ctttggaaga agcgatgttt gcatgatgcc cactccagaa aatcaaagca
    11881 gatttctaaa aagccagaca gcgaagtcct gatgatcggc gtgggccagt cgccgcaggc
    11941 aaatcagttt ttttgattgc ctgtccacca acgaaggagc ccctgaacca aatctcagga
    12001 gttcccatgc gaacattact gcttgctcta accattctgg ccatcgtgca ttgcggccca
    12061 aacaaaaagg attcctacta ctgcgatata acaagccaga gtctggacac ctgcgatggc
    12121 aaaacaatca aagtcaccgc aaatcattct gacaggattc agcaacatcc tgtgatcagt
    12181 ccagataagc aatcctactg ggacgtcaat ggccgcgact ggatcttcgt gagcaaaaac
    12241 gcaatcgatt gcaaaacagg catcgtggtt acgggtagac tggacgcaaa agtaggacca
    12301 tgcgatccaa ccgcccagaa tcacaaccag tattgcggaa cagcagtata cgtggacaac
    12361 tggaagtgcc aataaaattc gaaaaaaatc atttctattc aacggccggg gcgttagctt
    12421 atccagatgg agcagcccgg actggatcag tttctaccgg cggcttccag ctggtttgaa
    12481 caaacgcttg gccaacccac gcgcattcag gaacgctcct gggaggcact gaagtcagga
    12541 gctcctgctc tcatttctgc acccactgga tcaggcaaga ctctcgccgc cttcttttcc
    12601 atcctggacg atctttttag aaagagcctc gccgggaatc tcgcgcaaga atccaccgta
    12661 ctttacatca gccctctcaa agctctctcc aatgacattg agaaaaatct gaggataccg
    12721 ttagacggca ttgcgagaga gttggcagcc aggggcactc ctctttcttc gccaatcgaa
    12781 gtcatggttc gcactggaga tacgactcca gcagagcgtg cgcgcatgat gaagcagaag
    12841 ccgcacatca ttgtaaccac tccagaatcc ttctacgtaa tgctcacgag cgaacgcggg
    12901 cgcagcatgc tctcgaccgt ggacacgctc attgtggatg agatccatgc aatagtctca
    12961 tctcgcagag gctcgcacct ggcactatct attgaaagac tggccgcgct gacgcgtaaa
    13021 cctctccaga gaatcgggat ctccgccact gtgaaacctc tcgaccgtgt agcggagttt
    13081 ctgggcgcga aaaaagaagt acgaattcta gacgaaggcc acaaccgacc catggatgtg
    13141 aagatgatca ctccggatgt tgctctcgac gcggtgatga cgcaagaggg atgggcgcag
    13201 atccatgaaa agctaatcga actcatgaag tccgccagaa ccaccttgat ttttgtaaac
    13261 aatcgacggc tctgcgaaag actcgcacga caattgagcg atctgatcgg tcaggatctg
    13321 gtaggagcgc atcatggatc tctcgcacat gatcgcaggc gaaatgcaga gcaggctctg
    13381 aaagcaggaa ccctcaaagt gatggtggct acgtcttccc tggaactggg gatcgacatt
    13441 ggatctgtgg acctggtagt gcagatcgcc tctcccagat ccattcacgg atttttacaa
    13501 cgaattgggc gcgcagaaca tcacaaagac ggcgtatcgc gagccgttct tgttccttta
    13561 accagagatg acctggtaga atgcgcagct ctccttcgcg ccatcaagca gggaaaactg
    13621 gaagaaatcg aaatcccgca agcaccgctc gacattctgg cgcaacagct agtggcagag
    13681 cttgcgtgta gaagcatgac agcggatcag gcatttgatc ttgtgcgaag cgcgaaccct
    13741 tacaagacac tggagcgcaa caagttcgag caaatactcg aaatgctcag tgatgggtac
    13801 tcccttagat ttggaaggcg atcgcgctat gtgttttacg atcgagctct aggcacactg
    13861 gaagcccgga gaggcgctcg cctcagtgcg atcaccaatg gcggagcgat tgcagataat
    13921 tttgaatacg aagtagttct ggcagacgaa ggcgcgttcc tgggaacggt gcatgaggat
    13981 ttcgcgatcg aaagtatggc cggagatgtt tttcaacttg gaaaccagtt ctggaaaatc
    14041 gaccgcatca ccacaggcaa agtcctggta tcgcacgctc cgaacagtac tcccactatg
    14101 cctttctgga tcgcagaggc accgggcagg agcgatgaac tttccgtggc agtttccaat
    14161 ctgcgctccg actcgcagcc gcgcatgcag gaaggtctgg aaggtttcgc agaatggctg
    14221 gaggaagaga cgggcctcgg tggcgctgct gccactacat tgtttgaata tttgagagaa
    14281 ggcgtgctgg ttctcggaca aatgcccacg cgaaacaaga ttgttctaga acgatttttc
    14341 gatgaggcag gagacagtca catcgttcta cattcgccgt acggcacgcg catcaatcga
    14401 gcgtggggcc tggcgttaag aaagagattc tgcagaaaat ttaatttcga acttcaggcg
    14461 gcggccacag atgacgcggt acttttttct ctgagcaaga cacatagctt cgcgcttggt
    14521 gaagtcttct cctatttaaa aagtgaaatt gcagagaaga tcctggtgca ggcactactt
    14581 gccgcaccca tgtttgaaat tcgctggaga tggaacgcat cacgtgctct tgccatcctc
    14641 agacaatacg gaagcaggcg cactcctcct caaattcaaa aaagccaggc gcaggacctg
    14701 gtcgcacttg tcttcccgga tcaaatcgca tgcgcagaaa acctggcggg agacagagaa
    14761 gtgccggatc atcccctggt agaacagacc attc
//
LOCUS       DAOQJL010000017        14772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1945_length_14772_cov_4.463070, whole genome shotgun sequence.
ACCESSION   DAOQJL010000017 DAOQJL010000000
VERSION     DAOQJL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_14772_cov_4.463070"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..697)
                     /locus_tag="PLG78_01355"
     CDS             complement(8..697)
                     /locus_tag="PLG78_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE21779.1"
                     /translation="MAKLPHHPGKTYWPSRIVTWLGQNFMRLFFKIKVEGHGNFPESG
                     PLLILAKHQRLEDIPLGLGAVCTAMKRRDQWCVMKHSLADPKYFGFFLKCGGIPIDRR
                     DPQKSKGFLLLARHKLHEGNTVVIFPEQTFFPSKMGKGKVPGFRFVAGRPDSPIQVGC
                     IGFTYKKGIIRTEVTMRLGKTTTPYTKHDDAEHFLHDRMLEIAELSSLHYPHPRPEGR
                     KAGADMEEEEN"
     gene            774..1709
                     /locus_tag="PLG78_01360"
     CDS             774..1709
                     /locus_tag="PLG78_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21780.1"
                     /translation="MLLWAGVAIILVHSVLMLLLKPALNRIAPGQFHIDFTGPGWIFP
                     LFYFRARKFSLMLRGGEKRDRVYLEADHVQFWVSPFDLLRGRLVLWRMRLIHPVLEYV
                     NRQDSSEKNKLLPAPGRFLIKRGRIRHGRIKVTDETRTPRYELELSKIAVKNGELDVG
                     VPAEVFFTLSQGKASLGDGTLEVRSSKKSGWLVIEGTLGDITSMQGMPFFGSRFYLEL
                     AHTRDMETGRTLIEGQISGSPHDRNPAPFEFEIDWNDYRLTMDLGIQKLIEQVIKNAR
                     PQGISIKSGVILSSRRFFDLIKKDYGDAEATGGGA"
     gene            complement(1673..2794)
                     /locus_tag="PLG78_01365"
     CDS             complement(1673..2794)
                     /locus_tag="PLG78_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21781.1"
                     /translation="MRRSLISLLAVAGLLSCHVDRPFEEPKPGDRIVHCNGRAYYLDA
                     ICEDEKEYLDWATKAYPEYPKFSNLTRDVLQKITVETKDVDKAASLFYERSLRDRNNI
                     EMMEFIGVKEKELAKRVPDYTGEKVLLALVPGMFWMDNPEVGAAGEQLRELADQMGMT
                     SSIVPVEQTGTVEKNGKDICEFLQQQTDVKSIIVASISKGGGDMKKALQYCGNTPAFR
                     KVRAWFNIAGITAGSQLVNGINDTIAYKAEARAYFCFKGYNWDGMMSMRAANDAPLEF
                     EVSVPKHIMVVNVIAVPLFRYVSSRAKPYYQYMIKYGPNDGITLLADSYVPGGSTYIS
                     FRNDHYFRNPISEQKMQAFLVYIMSKPFQAPPPVASASP"
     gene            complement(2794..4002)
                     /locus_tag="PLG78_01370"
     CDS             complement(2794..4002)
                     /locus_tag="PLG78_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNE21782.1"
                     /translation="MLSSKLFRFILGFSILLPAASLTAQADVPFLAEYKASYLQMSAK
                     ARANPDSVGAQLMLTAPGPTVTTAFGHVALRVYTKLNDDTQDFYVDFGNYEESVAFVR
                     KFLSGSALFYTKAIPTTMAIDQWAVSGRGVITYKLVMTREEKARFLAAVEDAIKKTEG
                     GYTYHNFRQNCVTYIRDILEKTYATPIELNAAHLAEGDTWRSRVVKYSRAISWLQLGE
                     NYWFNYLTDQKKNGRDVVYLPYDLYQAVVDGGHINGDRLVLKDRWAVRFDRQDWSGWL
                     FIVVFAIGFLLQLAPGSWGTRGRASVFIFSGFTGLVVWVVTLFTEYDFMARNALFFAV
                     CPFDFALLSNRVMEKIRPWLKPFAFARVGLWILALVICLILPQKSFTALALSGLAWSC
                     FLISVLRGER"
     gene            complement(4083..4484)
                     /locus_tag="PLG78_01375"
     CDS             complement(4083..4484)
                     /locus_tag="PLG78_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNE21783.1"
                     /translation="MARIASLIFAMAIAGTIACSGGSGAPIVAAPGSSGMGGGSIMNN
                     TKQNANCTLGAEDCIGETYREQGTKGAVVGAKQGRSCANSILGLIASGDVSIKGAAAA
                     GQITKITSVDQEDNSLLSSVLVERCILVNGE"
     gene            complement(4580..5374)
                     /locus_tag="PLG78_01380"
     CDS             complement(4580..5374)
                     /locus_tag="PLG78_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21784.1"
                     /translation="MTKPVINPRAVSQKAAAFFCHRLAVYGALSFISFCVAPQKTRLP
                     DPIWGFYDENAAERIRSAYEVVSPNDVRALRALSEKECKLDRAVRGSFLGHDAYTIGG
                     FAEEKNPGLVCNWNEARELIFYGRFNDKHQPIIAVNWFLNQYLRSVTIRLDQKNVTVF
                     RAWSISHEKGAWRSRLVHVDIKQNAQPHTLRFVFNRLDGGLLERSEFLGDEPSIEYVG
                     LFFTSALKRGEPNCLFYRRGIKTEILSREACKIPDTVDPELVRVLP"
     gene            5373..5933
                     /locus_tag="PLG78_01385"
     CDS             5373..5933
                     /locus_tag="PLG78_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21785.1"
                     /translation="MHIRVIFLALLVSTITGCWSSRISRGNYREYPFYELPPYSSSRD
                     ALFVLTLPPCAKESQIREVLSANFRMGYEMDYLTSQKDLPENIRVSRSPGTVYYTFPV
                     RVKGAGVVGIQLEASFRKRDLSTEGVVFRISPKEYPAGQVHIFELACPAEKKITLHYE
                     TESVEKFRKRPDAIPDNIRGVLKNPQ"
     gene            6008..6874
                     /locus_tag="PLG78_01390"
     CDS             6008..6874
                     /locus_tag="PLG78_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21786.1"
                     /translation="MIRSTSAHKEDRLIELLDQGGNAKLRDKSGEPIISLAIRSGSDI
                     RAIESLLSHGASADEANSYGKTPLMWAAETNRVDVMKLLLAKGAHVDARAREGWSALF
                     YAAQADKVEAAEFLLSKGADFKTPDSAGRTCLMNAANVGSERLVNKFLDLGADIKART
                     ASKRNAFVWAADERQTNIMLLLLRRGTPINEADIGGMTPLYYATALPSMEVTLDLLNR
                     GANPNIASSMGRTPLMNVARMGRLDIVKLLVERGADVNMRDWFRKSAIVLARNEGHLD
                     VVQYLRSKGAVD"
     gene            complement(6886..7209)
                     /locus_tag="PLG78_01395"
     CDS             complement(6886..7209)
                     /locus_tag="PLG78_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNE21787.1"
                     /translation="MKAIFAFSLALFLIQCSTNQSGGYVAPSGSVMQNTTLNRDIPAV
                     TAVGGRTGEACVTAYFNIISSGDASIKAAAAIGGISTVKSVDYRIENLLGSVYAKTCT
                     IANGD"
     gene            7199..7990
                     /locus_tag="PLG78_01400"
     CDS             7199..7990
                     /locus_tag="PLG78_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21788.1"
                     /translation="MAFKEYLTKFFLKQGDGWSFMCNQKTGPRILACALSFLCACTTT
                     RVTHENRSPASGPDPVQLYKLKVHYEDLPAAIMQRVRARALANCRFEEATKTEFLGRA
                     QYRSGPFEGNVPVGKICLWNFERELFQEGAYDRQGALAGTATHWYGERLRQYRRRIGA
                     TEISRSWYWTANGGKWTSVLVELSIVDHAKNRRIDYVFSRDGSFLVSRRETLGEKKSG
                     LQYNAVDSRGKSCVFVDALGSEREIALPCPLPPSDVISGGESFED"
     gene            complement(7987..8514)
                     /locus_tag="PLG78_01405"
     CDS             complement(7987..8514)
                     /locus_tag="PLG78_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21789.1"
                     /translation="MRKIISVLLILQVLASCATVVTVKTTPVGAGVSIIYEGKERGVG
                     EATFETGSSIFADALLTFQKGGEMVTQQRMERRFSVGYFLLGYVSLFVTWLWIAPFKE
                     TQIVDLAPFLKNRPGYVAQPVLTGFTDTVTLKNGQVFAGVKAAVTADSIVMTTRDGKT
                     LVFGKADVLSIKKGN"
     gene            complement(8557..9495)
                     /gene="fliH"
                     /locus_tag="PLG78_01410"
     CDS             complement(8557..9495)
                     /gene="fliH"
                     /locus_tag="PLG78_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNE21790.1"
                     /translation="MATGKLVFKPAQIAAARDLVELEIPDKYKKFQGIEDTDEYEVDS
                     EGNVIEQYHGPTIEEIEAELERYRKETEEKIQKMLEEAHERARAIEEEGKSVAFNLIQ
                     ESKEKAEREEETARVNAEQLVERAKLEVERLIKEADMRVNEIEHEAYTKGYDAGREVG
                     FKRGQSEVRRLIDRIGTIVGQAIDVREEIIAASEKQMVDMILMVARKVIKDEVAERKE
                     VVLNNIREALKRIKDRDRVDIRVNFADLELTTAHKDELIKMMESLRKVNIYEDSRIDR
                     GGCIIETDVGSIDARISTQLKEIEEAIRNAQPMTGK"
     gene            9654..10427
                     /locus_tag="PLG78_01415"
     CDS             9654..10427
                     /locus_tag="PLG78_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE21791.1"
                     /translation="MSLLIEERIEHTIRLRLNRPDALNAMSRELLALLETRIQALSVD
                     PAIRALVLTGSGRSFCTGADLKERASMSPPEVRDFLIRVGHIFQAIESFPCPVICGIN
                     GFALGGGLELALACDFRIAQTDALLGLTETSLGIIPGAGGTQRLPRLIGVSRAKALIF
                     TARKVGASEALSIGLVDSVVNAEKLDEACVELASSISKNAPIAVRSAKFAINAGANVD
                     LSTGLAIERNAYEITIPTKDRIEALHAFREKRAARFTGE"
     gene            10464..10997
                     /gene="dcd"
                     /locus_tag="PLG78_01420"
     CDS             10464..10997
                     /gene="dcd"
                     /locus_tag="PLG78_01420"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNE21792.1"
                     /translation="MILTGPEIVRRMGTEIKIEPFDASLINPNSYNLRLNDELMIYKE
                     RALDMKKPAQTEMIKIPPEGIVIEPGTLYLGRTVEYTETFNLVPMLEGRSSIGRLGLF
                     VHVTAGFGDVGFRGFWTLEISAIQPIRIYPFVPICQIFYHTIEGEVLEYKSGKYQNNT
                     GIQPSLLFKELEKNNAT"
     gene            10987..11583
                     /locus_tag="PLG78_01425"
     CDS             10987..11583
                     /locus_tag="PLG78_01425"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE21793.1"
                     /translation="MRLRIVEDVRSIFRNDPAARGLEFLLYPCLHAIFWHRLVCHPLY
                     NVGLRFFARFFSQMSRFFTGIEIHPGAKIDGGFFIDHGMGVVIGETAIIGRDCMMFHG
                     VTLGGTGKHKGRRHPIIGNDVLIGTHATLLGPIHVGDHARIGAETVIINRDVPANCTV
                     VGTPGKIVKQGNKKLKRPESLPISQYRVEELEAEKNGT"
     gene            11620..12327
                     /locus_tag="PLG78_01430"
     CDS             11620..12327
                     /locus_tag="PLG78_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21794.1"
                     /translation="MSGMLEFYRGNSETPSGSLLFYCRVRGPNPFSPDAKLLVCHVVV
                     SYLGVRNNNFPVVVFPPSALTSEEEFFELLDLDEDSDVVRLEDFEIPEGKPEADYMSE
                     RLERFNDRVYEYVEIYRKQLDRKRKKHGQQRLPLFPEQPLLIQAPDMDDAHAIATIEQ
                     AITDGLQSKESIQAAVEFLKHQHPQLDMINLESAVNQEDLKLSALYLDKFKAILEEKY
                     EDAARIQVQIRNFNPAG"
     gene            12324..13856
                     /locus_tag="PLG78_01435"
     CDS             12324..13856
                     /locus_tag="PLG78_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE21795.1"
                     /translation="MSSGKRAEDLHVGLMEGAATKEGTARYFLDLKTQFAEYRDHSWF
                     RFQTPSHLALSRIGFGCYRVSAGARANVDALREAILTGVNVIDTAANYSDGSSEQMVG
                     DVIRQLVEAKRIERNQVAVVTKIGYIQGRNLGLLRTAPPPEVVKVRDDIWHCIHPDYI
                     KSQLQLARYRLGLRTIDFLLLHNPEYFLIDAQTRGVLADEARAQYVDRLRRAFEMMEE
                     MANRGIIQYYGISSNTLAHSSSSYTATRLDEVLSVAGPRFAAVQFPANLIENDFRYNR
                     SAGGGTVTSVALAHGLWTLGNRPFNANGPRGLIRLARLVDAPPDGGDSGIAEFQKMQG
                     RLMELEAQLHDLFGSAFQFDKTAPAFSDILERYRDRFTTTDQLRASVEQITHLFSPTA
                     RRIQTAVQTKNQEIGFNQYLKVVNAVIHYWERYVEILVHQRMEGLENLLGEHPALQGK
                     PLALQVLLILLSGDVPATALVGMRKIPYVRQLVQAFAEPSPPEREMLSLVGRALDLIE
                     KF"
     gene            13891..14112
                     /locus_tag="PLG78_01440"
     CDS             13891..14112
                     /locus_tag="PLG78_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE21796.1"
                     /translation="MLTKQQEEVVALYNQGLGLYKARKFKEARETFLKGLQILPEDGP
                     SKLYVERCDAYIKDPPGEDWDGVYVMKTK"
     gene            14114..14503
                     /locus_tag="PLG78_01445"
     CDS             14114..14503
                     /locus_tag="PLG78_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE21797.1"
                     /translation="MLKKKESPSITEGGPISTILGKETSFSGVMEFKKPLQINGHFEG
                     ELITDGVLVVGEGATVKANIRAGIVIVAGEVTGNIEARQKLEMLTSGKVYGNVKTAKL
                     QIADGVVFDGNCEMITDGQTTAAKASA"
     gene            complement(14473..>14772)
                     /locus_tag="PLG78_01450"
     CDS             complement(14473..>14772)
                     /locus_tag="PLG78_01450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE21798.1"
                     /translation="EQCAFFVLPSLYEGFGLSLLEAMARGKHCIASDLGCFHEIGGSQ
                     VTYLGSKDVHAWRDALADAVSKHRSGKLRPTKIKLSDWSWRNTALVHADAFAAVV"
BASE COUNT         3779 a         3785 c         3710 g         3498 t
ORIGIN      
        1 tgcttcgcta attttcctct tcttccatat cagcccctgc cttgcgtcct tcagggcgcg
       61 ggtggggata atgcagactg ctcagttccg ctatctcaag catgcgatca tgcagaaagt
      121 gttccgcatc atcgtgtttg gtgtaaggtg tggttgtctt gccaaggcgc atggtcactt
      181 ccgtgcgaat gattcctttc ttataagtga aaccaatgca tcccacctga atcggactat
      241 ccggtcttcc tgctacaaat cgaaagccag gaaccttgcc tttgcccatc ttgctcggga
      301 agaaagtctg ctctggaaaa atcacgactg tgtttccttc gtgaagcttg tggcgggcca
      361 ataacaggaa acccttgctc ttctgcggat ccctcctgtc gatcgggatg ccgccgcatt
      421 tcaaaaagaa tccgaagtat ttaggatccg ccagagagtg cttcatgaca caccattgat
      481 ctcttctctt catcgcagtg cagaccgcac cgagacccag tgggatatct tcgagcctct
      541 ggtgcttggc cagaatcaag agaggaccgg attccgggaa attgccatgc ccttctacct
      601 tgatcttgaa gaaaagccgc atgaaattct ggccaagcca ggttacgatg cgagaaggcc
      661 agtatgtttt gccgggatgg tgaggaagtt tagccacgac cgatgctcga tttttgttcc
      721 tttcgatcaa gctcgaattg acagcacagc aatcgcacga gacataaaat cccatgcttc
      781 tctgggccgg cgttgccatc attctcgtgc actcagtcct gatgcttctg ttgaagccgg
      841 cactcaatcg aatcgccccc ggccagtttc acatcgactt caccggaccg ggctggattt
      901 tccccctctt ctactttcgc gcgcgaaagt tctctttgat gcttcgcgga ggagaaaaaa
      961 gagatcgcgt atatctggaa gcggatcacg tacaattctg ggtttcgcct tttgatttgc
     1021 tcagaggccg gttggtgctg tggcgaatgc gattgattca tcctgttctg gaatacgtaa
     1081 acaggcagga ctcttcggag aaaaacaaac tactccccgc acccggaaga tttctaatca
     1141 agagaggcag gattcgtcac ggacggatca aagtgacaga tgaaacgcgc accccgcgct
     1201 atgaactgga gctcagcaaa attgcagtga agaacggaga actggatgtg ggtgtcccgg
     1261 cggaagtatt cttcactcta tcacagggca aagcatccct gggagatggc actctggaag
     1321 ttcgctccag caagaaaagt ggctggctgg tgattgaagg aactctcgga gatatcacaa
     1381 gcatgcaggg catgcccttc ttcgggagca gattctatct ggaacttgcg cacacgcgtg
     1441 acatggaaac agggcgaact ttgatagaag gtcagatatc aggatcgcct catgatcgca
     1501 atcctgcgcc ttttgaattt gaaatcgatt ggaatgacta caggctaacc atggatctag
     1561 gaatccaaaa actgatagag caagtcatca agaacgcaag accccaggga atcagcatca
     1621 agagcggcgt gattttatcc agccgccgct tttttgatct gatcaagaaa gattatggag
     1681 atgcggaagc aacaggcggt ggtgcctgaa acggcttgga catgatatag accaggaacg
     1741 cttgcatctt ctgttcagaa atcggattgc gaaagtaatg atcgtttctg aatgaaatgt
     1801 aggtagatcc tccaggaaca tagctatccg ccagcaaagt aattccatcg tttggaccgt
     1861 acttgatcat gtattgatag tatggttttg ctctggatga aacataccgg aacagaggaa
     1921 ctgcgattac attgaccacc atgatgtgtt tgggtacaga cacttcgaat tcaagaggcg
     1981 catcattggc cgccctcata gacatcattc cgtcccagtt ataacccttg aagcagaagt
     2041 aggcccgagc ttccgctttg taggcaattg tgtcgttgat tccgtttaca agctggcttc
     2101 ccgctgtgat tccagcgatg ttgaaccagg cgcgaacttt gcgaaacgca ggagtgttgc
     2161 cgcagtattg cagagctttc ttcatatccc cgccgccttt gctgatagaa gcgacgatga
     2221 tgcttttgac gtctgtttgc tgttgcaaga attcgcaaat gtctttgccg tttttttcca
     2281 cggtcccggt ttgctcgact ggaacgatgg aagacgtcat tcccatttga tctgccagtt
     2341 cgcggagctg ttctccagcc gcgcccactt caggattgtc catccagaac attccaggca
     2401 cgagagcgag aaggactttt tctccagtgt agtcagggac tcgcttcgca agttcctttt
     2461 ccttcacacc aatgaactcc atcatttcaa tgttgtttcg atcccgcagc gaacgctcgt
     2521 aaaatagaga agccgccttg tccacgtcct ttgtttccac ggtgatcttc tgcagaacgt
     2581 ctctggtgag gttgctgaat tttggatatt ctggatacgc tttggtggcc cagtccaggt
     2641 attccttctc atcttcgcaa attgcatcaa gataataggc tctgccgttg cagtgaacga
     2701 tccgatctcc cggctttggt tcttcaaacg gtctgtccac atgacaggac agcaatcctg
     2761 cgacagcgag cagggaaatc aaggaccggc gcatcaacgt tctcctctaa gcacagatat
     2821 aagaaagcaa ctccaggcca gaccggaaag tgccagtgca gtgaatgact tctgaggaag
     2881 gatcaaacaa atcacaagag caaggatcca caggccgacg cgcgcaaatg caaaaggttt
     2941 aagccagggc cggatctttt ccataacgcg attggatagt aaagcaaagt caaacggaca
     3001 gacggcaaag aacaaagcat tgcgtgccat gaaatcatat tcagtgaaga gagtcacgac
     3061 ccagactaca agtcctgtga agccgctgaa aataaataca gaagcacgac ctctcgtccc
     3121 ccaggagcct ggagcaagct gcagtaaaaa tccaattgca aaaactacta tgaagagcca
     3181 gccggaccaa tcctggcgat cgaatcgcac agcccagcga tccttcagaa ccagtctatc
     3241 cccgttgata tgaccaccat ccactactgc ctggtataga tcatacggaa gataaactac
     3301 atctctgcca tttttctttt gatcggtcaa ataattgaac cagtaatttt cgccgagctg
     3361 cagccaggaa atggctctag aatatttcac tacgcgagaa cgccatgtat ccccttctgc
     3421 gagatgagca gcgttgagtt caatgggggt cgcgtatgtt ttctccagaa tatcgcggat
     3481 gtaggtcacg caattctgac ggaagttgtg gtatgtgtaa ccgccttccg ttttcttgat
     3541 cgcatcttcc actgccgcca aaaaccgtgc tttctcttct ctggtcatga ccagtttgta
     3601 tgtgatgact ccgcgaccgc tcacagccca ctgatcgatg gccatggtag taggaattgc
     3661 tttcgtatag aaaagcgcgc tgcctgagag gaatttcctc acgaatgcaa cagactcctc
     3721 atagtttcca aaatcaacgt agaagtcttg agtgtcgtcg ttgagtttcg tgtaaaccct
     3781 gagggcaaca tgaccgaatg cagtggtcac agttggacct ggtgctgtga gcatgagctg
     3841 agcgcccact gaatcaggat tcgcgcgtgc cttcgcagac atctgcaaat aagaagcttt
     3901 gtattctgcg agaaacggaa cgtccgcctg cgccgtgagg gatgccgcgg gaagcaaaat
     3961 agaaaaaccg aggatgaaac ggaacaattt agatgatagc attgaaaggt gtaaaagtgt
     4021 gaggagggaa gtgaaaagaa aagccttgcc ggtcgccaga ggcgaccgac aagctagagt
     4081 gattactctc cgttaacgag aatgcaacgc tccacgagta cagagctcag gaggctgtta
     4141 tcttcttggt caacagaagt gatcttcgtg atttggcctg ctgctgcagc acctttgatg
     4201 gacacgtcgc cagaagcgat gaggcccagg atgctgttcg cgcaggaacg gccttgctta
     4261 gcaccaacta cagcaccttt agtgccttgt tcgcggtaag tttcaccgat gcagtcttct
     4321 gcaccaaggg tgcagttagc gttctgcttg gtgttgttca tgattgaacc gccacccatt
     4381 ccagatgaac ctggagcagc tacgatcggg gcgccgcttc cgccggaaca tgcgattgta
     4441 ccagcaatag ccatagcgaa gatcaggctt gcaattcttg ccatttttgt tttctcctat
     4501 ttgattggag acattgagtc tccgcgtagc tataaggctc tcctgtcctt tttctgcaag
     4561 gattttttta tcgaatggct cagggcagga ctcgaacgag ttctggatcc acagtatccg
     4621 ggattttgca ggcctcgcgg gacagaattt cagtcttgat gccccgacga taaaacagac
     4681 aatttggctc gcctcttttg agtgcgctcg tgaaaaagag accaacatat tcaatagaag
     4741 gttcatcgcc caggaactct gatctttcga gcaaaccacc atccaatctg ttaaaaacga
     4801 agcgaagagt gtgtggttgt gcgttctgct tgatatctac gtgtaccaga cgtgatctcc
     4861 aggccccttt ttcgtgcgaa atagaccagg cgcgaaacac agtgacattt ttctgatcca
     4921 gacgaatggt cacgctgcga agatattgat tcaaaaacca gttcactgct atgattggct
     4981 gatgcttatc attgaagcgc ccgtaaaata tcagctcccg tgcctcattc cagttgcaga
     5041 ccagtcccgg gttcttttct tccgcgaagc cgcctatggt gtaggcatca tgtccgagaa
     5101 aactgccacg cacagctcga tccagcttgc attccttttc agaaagagct cgcagggcgc
     5161 gaacatcgtt cggagaaaca acttcatagg cggaacgaat tcgctctgcc gcgttctcat
     5221 cgtaaaagcc ccagattgga tcggggagcc gcgtcttctg gggagcaacg caaaaagaaa
     5281 tgaaagaaag cgcaccataa accgccagcc ggtggcaaaa gaaggcagct gctttctgac
     5341 tgacagcgcg cgggttgatc acaggtttgg tcatgcacat acgcgtcatt tttcttgctc
     5401 ttctcgtttc aacgatcact ggatgctggt cgagcaggat ttcccggggg aactaccgcg
     5461 aatatccatt ttacgaactg ccgccttact ccagctcgag agacgctttg tttgttttga
     5521 ctcttcctcc ctgtgccaaa gaaagccaga ttcgagaagt gctctctgcg aactttcgga
     5581 tgggctacga aatggactac ttaacgtctc aaaaggatct tcctgaaaac attcgcgtat
     5641 cgcgcagccc tggaaccgtt tactatacct ttcctgtgcg cgtaaagggc gcaggcgttg
     5701 tgggcattca actcgaagct tcctttagaa agcgcgacct gtccacggag ggtgttgtgt
     5761 ttcgcatatc ccccaaagaa tacccggcgg gccaggttca tatcttcgaa ctggcatgcc
     5821 ccgcagaaaa gaaaatcact ctgcactatg aaacagagtc cgtcgagaaa ttcagaaaaa
     5881 gaccggatgc aatcccggat aacattcgcg gcgtgctcaa aaatccacaa tgacgcgcaa
     5941 aacgttccgc atcgttccat cgattttcct tttgtggtct ctgatatctt gcagcacgga
     6001 ccgcgaaatg attcgctcca cttccgcgca caaagaagac cgactcattg aactgctcga
     6061 ccagggcgga aatgcaaaac tacgcgataa gagcggagaa cccatcatca gcctggccat
     6121 acgatctggc agtgacatca gagccatcga aagcctgctt tcgcacggag cgagcgcaga
     6181 tgaggcaaac tcctatggaa agacaccttt gatgtgggcc gcagaaacga atcgagtcga
     6241 tgtgatgaag ctgctccttg cgaaaggcgc acacgtcgat gcaagagcgc gcgaaggctg
     6301 gtctgctctg ttttacgcgg cacaggcgga taaggtggaa gccgcagaat ttcttctttc
     6361 aaaaggcgcg gatttcaaaa cgccagattc agcaggacgc acctgtctga tgaatgcggc
     6421 aaacgtcggc agcgaaagac ttgtgaataa gttcctggac ctgggtgcag atatcaaagc
     6481 acgaaccgca tccaaacgaa atgcattcgt ctgggcagca gatgaaaggc aaacaaacat
     6541 catgcttctc ttgcttcgcc ggggaactcc gatcaacgaa gcagacattg gcgggatgac
     6601 tcctctgtac tacgcgaccg cccttccctc catggaagtg actctagatc tattgaaccg
     6661 aggagcgaat ccgaacattg catctagcat gggaagaact ccgctcatga atgtagcgcg
     6721 aatgggaaga ctggatatag ttaagctgct cgtagaaaga ggagcagatg tgaatatgag
     6781 agactggttt agaaaatcag caatcgtact cgcccgcaat gaaggtcacc tggacgtagt
     6841 ccagtatctg cgctccaaag gggcggtaga ttagttgcag aacaatcaat cgccgttggc
     6901 gatggtgcag gtcttcgcgt acacgctacc gagcaggttt tcaattctgt agtctacgga
     6961 cttcacagtg ctgataccac cgatcgcagc cgcagccttg atgcttgcgt ctccgctgga
     7021 aataatgttg aagtatgctg taacgcatgc ttctccagtg cgtccgccca cggcagttac
     7081 tgctggaatg tcacgattca gagttgtatt ctgcatcaca gatccgctgg gtgcaacata
     7141 gccaccggat tgattggtgg agcactggat caggaacaga gcaagtgaaa atgcgaaaat
     7201 ggctttcaag gagtacctca cgaaattctt tttgaagcaa ggtgatggct ggagtttcat
     7261 gtgcaatcaa aaaaccgggc ccagaattct ggcatgtgcc ctctcctttc tgtgcgcatg
     7321 cacaaccaca cgggtgacac atgaaaatcg gtctcccgct tctggcccgg atccagtaca
     7381 actttacaaa ctgaaagtac actacgaaga cttgcctgcg gcgatcatgc aaagagtcag
     7441 agcacgggcc ctggcaaatt gcaggttcga agaagcgaca aagaccgaat ttctcggacg
     7501 agcacaatac agatcaggtc catttgaggg aaacgttccg gtcgggaaga tttgcctgtg
     7561 gaattttgaa cgcgaactct tccaggaggg tgcctacgac cgccagggag cgctcgctgg
     7621 aactgctacg cactggtatg gagaaagatt gagacagtat cgacgcagga taggagctac
     7681 agaaatcagc cgatcctggt actggactgc gaatggcggg aaatggactt cagtgcttgt
     7741 agagctcagc attgtggatc acgcgaagaa cagaagaatc gactatgtct tctccagaga
     7801 tggatctttc ctggtctcga gacgcgaaac cctgggcgaa aaaaaatcgg gacttcagta
     7861 caacgcagtc gattcgaggg gtaagagctg tgtattcgtg gatgcgctgg gaagtgaaag
     7921 ggaaatagct cttccctgtc ctcttccgcc ttctgatgtc atctccggcg gagagtcttt
     7981 cgaggattag tttcccttct ttatagatag aacgtcagct ttaccaaaaa cgagagtctt
     8041 tccgtctctc gtagtcatca cgatagaatc cgcagtgact gctgccttga ctccagcgaa
     8101 cacctggcca ttcttcagcg tcactgtatc tgtgaatcca gtgagcactg gctgcgctac
     8161 atagcccgga cgatttttca agaaaggagc gagatccaca atctgcgtct ctttgaaagg
     8221 agcaatccag agccaggtaa caaagagcga gacatagcca agcaggaagt agccaacaga
     8281 aaaacggcgc tccattctct gctgggtgac catttcaccg cctttctgaa acgtaaggag
     8341 agcgtccgcg aatatgctgg agccagtttc aaaagtggct tcccctacac cgcgttcttt
     8401 tccttcgtag atgatggaaa cgccggcacc gactggagtg gtcttcacag tgacaacggt
     8461 ggcacaggat gccaggacct ggagtattaa taagactgat atgattttgc gcacggtcta
     8521 cggtgtagag caccgtagac caggacaacc atttttctat ttgcctgtca tgggctgcgc
     8581 attgcgaatc gcttcttcga tttccttcag ctgcgtagaa atacgagcgt cgatcgatcc
     8641 aacatccgtc tcgatgatac atccgccgcg gtcgattcga gaatcctcgt aaatgtttac
     8701 cttccgcagt gattccatca tcttgatgag ttcgtccttg tgcgcggttg taagttccag
     8761 gtccgcaaag ttgacccgta tatccacacg gtcacgatcc ttgatccgct tgagagcttc
     8821 acggatgttg ttgagcacca cttctttccg ttcggccact tcgtctttga tcaccttgcg
     8881 ggcaaccatg agaatcatgt ccaccatctg cttttctgat gctgcaatga tttcttcacg
     8941 cacgtcaatc gcctggccta cgatggttcc aatgcggtca atgagacgtc gaacttcgga
     9001 ctggcccctt ttgaaaccaa cttcgcgacc tgcatcgtaa ccttttgtgt acgcctcgtg
     9061 ttcgatttcg ttgacgcgca tgtccgcttc tttgatcagg cgttctactt ccagtttggc
     9121 tctttctacg agctgttctg catttacgcg cgccgtttct tcttcacgct ctgctttttc
     9181 ttttgattcc tggatgagat tgaatgctac gctcttgcct tcttcttcaa tggcgcgggc
     9241 gcgttcatgc gcctcttcga gcatcttctg aatcttttct tcggtttcct tacgatatct
     9301 ttcgagctct gcctcgatct cttcgatcgt cgggccgtgg tattgctcga ttacgtttcc
     9361 ttccgagtct acctcgtatt cgtctgtatc ttcgattccc tggaacttct tgtatttatc
     9421 cggaatctca agctcgacca gatcgcgtgc tgccgcaatc tgcgccggtt tgaatacgag
     9481 tttgcctgta gccatacgat tcctagcctg cttgcttcgt tctaatgtac aaggcttttt
     9541 tggccctggc tctaatatcg gccaattttt acagatcaaa gagccagcca ggcacccatc
     9601 ggtcaaaagg cgaaagtcct tgtccgggcc cctgagcaca aaaacaatgc agcatgagcc
     9661 tactgatcga agaaagaatc gagcatacca taagactgcg cttgaataga cctgacgcgc
     9721 tcaatgcaat gagtcgcgag cttctggcat tgctcgagac cagaatccag gcgctctctg
     9781 tggacccggc catacgagct ctggttctaa cggggtccgg acggtctttc tgcacaggtg
     9841 ccgatctcaa agaaagagca tccatgagcc cgccagaagt gcgcgatttt ctcatccgag
     9901 tcggtcatat cttccaggcc attgaatcct ttccctgtcc tgtcatctgc ggcatcaacg
     9961 gctttgcact cggaggcggc ctcgagctgg ctctggcatg cgactttcga atcgcccaga
    10021 cggatgcgct cctcgggctt acagaaactt ctctgggaat cataccgggt gccggtggca
    10081 ctcaaagact gcccagactc attggagtgt cccgtgcgaa agccctcata ttcaccgcac
    10141 gcaaagtcgg cgcatccgaa gcattgagca taggtctggt tgattcagtc gtaaacgcgg
    10201 aaaaactcga tgaagcttgc gtcgaactgg ccagctcaat ttcaaaaaat gctcccattg
    10261 cggttcgatc tgcaaagttt gccataaacg caggcgcgaa tgtagacctg tccactggcc
    10321 tggcaatcga gagaaatgca tacgaaatca caattccaac gaaggacaga atcgaagcac
    10381 ttcatgcgtt ccgcgaaaag agagcagcca ggttcaccgg cgaatagtag agaaacaaca
    10441 actgaagcat acagaggcaa gagatgatct taacggggcc ggaaattgtt cgcaggatgg
    10501 gaacagaaat caagatcgaa ccattcgatg cgtctctgat caatccgaat tcatacaacc
    10561 tcagactcaa tgacgagctg atgatctaca aagagcgagc gctcgacatg aagaagccgg
    10621 cgcaaacaga aatgatcaag atcccaccgg aagggatcgt cattgagcct ggaactctct
    10681 atcttggtag aacagttgag tatacggaaa cattcaatct ggtgcccatg ctcgaaggca
    10741 gatcttccat tggaagactc ggtctattcg tgcacgtaac tgccgggttt ggtgatgttg
    10801 ggtttcgcgg attctggact ctggaaattt ctgccatcca gcccattcgc atttatcctt
    10861 ttgttcccat ctgtcagatt ttttatcata ctatagaagg cgaagtgctc gagtacaagt
    10921 caggaaaata ccagaacaac actggcatcc agcccagcct tctgttcaag gaactagaaa
    10981 agaacaatgc gacttagaat tgtcgaagac gtacgctcca tctttcgaaa tgacccggca
    11041 gccagaggac ttgagttttt gctctatcca tgtctgcatg cgattttctg gcacaggcta
    11101 gtttgccatc cactctacaa cgtaggtctg cgattcttcg cgcgcttctt ctctcagatg
    11161 tccaggttct tcaccggaat cgaaatccat ccgggtgcga aaattgacgg cgggttcttc
    11221 atcgatcatg gaatgggtgt agtgatcgga gaaacggcga tcattggcag ggactgtatg
    11281 atgtttcacg gagtcaccct gggtggaacc ggcaaacaca aagggagaag acatcccatc
    11341 attggaaatg atgtgctcat tggcactcat gccactcttc tcgggcccat tcacgtaggc
    11401 gatcatgcgc gaattggagc ggaaactgta atcatcaacc gggacgtgcc tgccaactgt
    11461 actgtggtcg gaactccggg aaaaatcgtc aaacagggca acaaaaaact gaaaagaccg
    11521 gagtcccttc cgatcagcca gtatcgcgtg gaagagctcg aggcggaaaa gaatggaact
    11581 tgacgccggc accgttttgc cttataatga aagcctctag tgagcggaat gcttgagttt
    11641 tatcgcggca attcggaaac accatccgga agccttctgt tttactgccg ggtgcgcggg
    11701 cccaatcctt tttcgcccga cgcgaagctc ctggtctgcc atgtggtggt cagctacctg
    11761 ggtgtgcgaa acaacaattt cccggtcgta gtatttcccc cgtcagctct tacctcagaa
    11821 gaggaattct ttgaactgtt agatctggac gaagacagcg atgtagttcg cctcgaagat
    11881 ttcgaaattc cggaaggaaa gccagaagcg gactacatga gtgaacgcct ggagcgtttc
    11941 aatgaccggg tttatgaata tgtggaaatc tatcgcaagc agctggatag aaagcgcaaa
    12001 aaacacgggc agcaaagact gccgcttttt cccgaacagc ctctcttgat tcaagcaccg
    12061 gacatggacg atgcgcatgc catcgcaacc attgagcagg caatcacgga cgggcttcaa
    12121 agcaaggaaa gcatccaggc cgcggttgaa tttctaaagc accagcaccc gcaactcgat
    12181 atgatcaacc tcgaaagcgc ggtcaaccag gaagacctga aactcagtgc tctgtacctg
    12241 gacaagttca aggcaattct cgaggagaag tacgaggatg ccgctcgcat tcaggtccaa
    12301 attcgaaact tcaatccagc tggatgagct caggaaaaag agccgaggat cttcatgtcg
    12361 gcctgatgga aggagcggcc acaaaagaag ggaccgctcg ctacttcctg gatctgaaaa
    12421 cacaattcgc agaatacaga gaccattcct ggtttagatt ccagacgcca tcgcatctgg
    12481 cactttcgcg catcgggttt ggctgctaca gagtgagtgc tggcgcgcgc gcaaatgtag
    12541 acgctctccg ggaagctatc cttacaggag tcaatgtgat cgataccgcc gccaattact
    12601 cggacggatc ttccgagcaa atggtgggag atgtaatcag acagctcgtg gaagcaaaaa
    12661 gaatcgagcg caatcaagtt gctgtggtta caaagatcgg atacatccag ggaagaaacc
    12721 tcggcctgct gcggaccgct cctccgccag aagttgtgaa agtgcgcgat gacatctggc
    12781 actgcatcca tccggactac atcaaatcgc aactgcaact cgcgcgctac agactggggc
    12841 ttcgcaccat cgacttcttg ctacttcaca atccagaata ctttctcata gacgcacaaa
    12901 cacgaggcgt cctggcagat gaggcccgtg ctcagtacgt ggatcgattg cgccgcgcct
    12961 ttgagatgat ggaggaaatg gccaatcgtg gaatcattca gtactacgga atatcctcca
    13021 acactctggc ccattcatct tccagttaca cggccactcg tctcgacgaa gtcctctcgg
    13081 tagctggccc tcgctttgcg gcagttcagt ttccagccaa tctcattgag aacgacttta
    13141 gatacaatcg ctctgcagga ggaggaactg taacgtcagt cgctcttgcc cacggactct
    13201 ggactctggg caatcggcca ttcaatgcaa acggtccgcg cggactgatt cgcctggcac
    13261 gcctcgtgga tgctccaccg gacggaggag attcaggaat tgctgagttt caaaaaatgc
    13321 agggtcgttt gatggaactc gaagctcagc ttcatgatct gtttggttct gccttccagt
    13381 tcgacaaaac cgcgccggcg ttttctgata ttttagagag ataccgcgat agattcacga
    13441 ccacagatca actgcgtgcg tctgtggagc agattacgca tttgttcagc ccgaccgccc
    13501 gaagaatcca gacagcagtt caaacaaaaa atcaggaaat tggattcaat cagtatctaa
    13561 aagtagtcaa cgctgtgatc cactactggg agcgctatgt ggagattctg gttcaccaga
    13621 gaatggaggg tctcgaaaat ctcctgggag aacatcccgc tctgcagggc aaacccctgg
    13681 cccttcaagt gctcctgatt ctgttgtccg gagatgtgcc cgccacggca ctcgtgggca
    13741 tgcgaaaaat tccatatgtt cgccagctcg tccaggcatt cgcggaacca tctccgccgg
    13801 aaagggaaat gctatccctg gtgggccggg cactcgatct aatcgagaaa ttttgattgc
    13861 aagcgcgccc cccgactgag aagaacaaat atgctcacaa aacagcagga agaggtcgta
    13921 gcgctttaca atcagggcct cgggctctac aaagcgcgca aattcaaaga ggcacgcgaa
    13981 acgttcctca aaggtctgca gatcctgcca gaagacggtc catccaaact ctacgtggaa
    14041 cgatgtgacg catacatcaa agacccgcca ggtgaagact gggacggagt ctatgtaatg
    14101 aagacaaaat aacatgctca agaaaaaaga atccccctcc atcactgaag gagggccgat
    14161 ttccaccatc ctgggaaagg agacttcctt ctccggcgtg atggaattca agaaaccact
    14221 gcagatcaac ggccactttg agggcgaact catcacagac ggcgtgctcg tggtcggaga
    14281 aggcgcgaca gtaaaagcga acattcgcgc gggcatcgtc attgtggccg gggaagtcac
    14341 cgggaatatt gaagccaggc aaaagctgga aatgctcaca tcaggaaagg tctacggaaa
    14401 cgtgaagact gcaaaattgc agatcgcaga cggagtcgtc tttgatggca attgcgaaat
    14461 gatcacagac ggtcagacta cagctgcaaa agcatctgcg tgaaccagcg ccgtatttct
    14521 ccaggaccaa tcgcttagtt ttatttttgt aggacgcagt tttccggaac gatgcttcga
    14581 gaccgcgtcc gcaagagcgt ctctccaggc atgcacatct tttgacccaa gataggtaac
    14641 ctggcttcca ccgatctcat gaaagcaacc aaggtcagat gcaatgcaat gcttgcccct
    14701 ggccatcgcc tctaatagag aaaggccaaa cccttcatac agagagggaa gaacaaagaa
    14761 tgcacattgt tc
//
LOCUS       DAOQJL010000018        14708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_1956_length_14708_cov_4.412544, whole genome shotgun sequence.
ACCESSION   DAOQJL010000018 DAOQJL010000000
VERSION     DAOQJL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_14708_cov_4.412544"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1441
                     /locus_tag="PLG78_01455"
     CDS             137..1441
                     /locus_tag="PLG78_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21799.1"
                     /translation="MESSSTMPCALLDFFAPVSSICAKFRFRRMFAFLYFVRRFCFYG
                     SAFFFCSCVAGCRFSAGAPESIWRALFVYDVAFLDLCGPQVSEVNVLSSDNAARVRLY
                     FQFMQSLGSIRSPSITTFGSVGANTYQMGCLARNGKIYAMPDNATQVLEIDPDTRTVS
                     NVPGTVAGANPKYSGCALQANNGKIWGAARNPTQILEVDPTTRSTFQDIAGSLPFASF
                     LGAKTGPDGHIYAFRDSAGAVLDYNPNTRTSTSFAAIPGYGEAVLATNGKFYGVPNNT
                     NPPVGEIDPIARSLRTGGGTYGVGAYDGGSIAPNGKVYMTPLAATTVLEVDPDLFPAS
                     NAFSQFGSTFGNQYSNGILAPNGKIYAPPRTGTSVLVTDPLNRTTSTIGTTSGVYIGA
                     TMGLNGKIYFIPNGATTIAELDTGSAGTFCPNVILSSYINSN"
     gene            complement(1438..2526)
                     /locus_tag="PLG78_01460"
     CDS             complement(1438..2526)
                     /locus_tag="PLG78_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21800.1"
                     /translation="MDALWKDLSELLTAFGEIQDRLDPTLFSQSASRIEPLAAKLELS
                     RNEAERSTQNAPSVDVLLRSASQALQGAARFLEAPAGPHGIMKAFQALRPIHRSEEIL
                     YSLAGQFEEVSKFFLPASHRENRELLKRLSQSPSGDIASLSDASDCGIIHFNNERGKR
                     GGWSIYVPEYYTKEKRWPLVIALHGGSGHGADFLWSWLRDARARGMLLASPTSLDRTW
                     FLHHPGTDVVGLNSMLSQISEKWNVDTKRLLLTGISDGGTYSMVLSTVRQSPFTHYAP
                     VAAAVHALLNREGIVEAPVADLKIFQVHGARDWMFPVEKARLAASALKNAGANIVYRE
                     VEDLSHNYPRDLNAEILDWFTRSGSDLY"
     gene            2837..2995
                     /locus_tag="PLG78_01465"
     CDS             2837..2995
                     /locus_tag="PLG78_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21801.1"
                     /translation="MKQGARFVDQGRIITSAGISAGIDMSLHIVERYFGKEASRATAR
                     RMEYDFIS"
     gene            complement(3015..4052)
                     /locus_tag="PLG78_01470"
     CDS             complement(3015..4052)
                     /locus_tag="PLG78_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE21802.1"
                     /translation="MSRFEIPREMQALELSANGSTVREACANLKVVRKPVPVPTKGQV
                     LVRVEAAPCNPSDLSYLQGHYAVDRPLPAVPGFEASGVVVKSGGGLIGSWLLGKRITC
                     AGQSPRDGTWAEYFIADAMSCLPLRRGVSFEQGCSLIVNPLTAFALLERAREFGSKAL
                     VQDAAASQLGRMIAALAREQGIPLINIVRKKEHVELLKNAGNAHVLNSSENDFDAQLQ
                     KLAQDLKATTAFDCISGAMSARVLQALPSRSQIIVYGSMSGEDATAKSKDIVFFRKNV
                     TGFHLASHIKERGTLWVLKTARQVQKLVERKIFHTEFSARLTLAQAPEGIAAYAENMT
                     SGKPVILPRGV"
     gene            complement(4141..5289)
                     /locus_tag="PLG78_01475"
     CDS             complement(4141..5289)
                     /locus_tag="PLG78_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21803.1"
                     /translation="MKKILTFTFLLLAAASCTPAHPRDTGHWDSVARYLAGMEVQEDP
                     NLAQLSRRPEWVTHKQSMDKMFSGLEENYLSKMKPWAEREIGNPGTPVLFYPFGGPEI
                     IISQTFFPHVREYIMCGLEAPGLPPDPTKVTAARLNPVLTNLRESLKWLTSYTYFQTI
                     HMDSQLRYTDLSGTLPLMLVFLARTGNRVTDVTGVILGNNGTLYELEENSPAPGRIPE
                     SSIKTTGIRGARITFVKNGTERQAYYFSFDASDPAMKTRSYFFDFVKSRGERSVMLKA
                     ASYLLHWGNFPTLKAFILNESRLILSDDTGIPFKDWDRKTWNLTLYGNYQAPIPQFAG
                     QIQYDMLRAYSNKKDVKELPFNIGYTAAWKPGISSLVLARRMDQTEGK"
     gene            5574..6296
                     /locus_tag="PLG78_01480"
     CDS             5574..6296
                     /locus_tag="PLG78_01480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21804.1"
                     /translation="MSCVTGLEYVEQDTFGRLSWIIPIAAVMMLADFLFFALVYSSPG
                     GNLITAQVGLASSVIALTLSTALLYLLRTIVDGAILRVEALNLSLSRFVPAEFLRLLN
                     RQSVEQISLGESKEATMSVLFSDIRDFTAIAEGNSPENNFKFINDYLKRMGPVIRDHN
                     GFIDKYIGDGILALFEHADDAVRAGLAMIAELENFNRERSEPVEIGIGIHTGTHHARH
                     NRRRASHGKHCDRRHGQLSIAH"
     gene            6217..7005
                     /locus_tag="PLG78_01485"
     CDS             6217..7005
                     /locus_tag="PLG78_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21805.1"
                     /translation="MLGTIGEELRMENTVIGDTVNSASRIEGLTKVYYTRFLVSRDTL
                     SAMQDHTGIRSRLVDITLVKGKRKPLYVHEVICGEEESIMAARYSGGALLEKGVHAVR
                     SGDYISGKNIFEEYLSQYPFDRTAKVHLQRCARVFNQFKGKSIFDKYGDISTVGSLVE
                     LFYSKILEDDLLSDFFTNTNMVRLKGHQTRFIAMLMGAPIRFDTCALEYAHAHLPITR
                     AHFQRVGLLLENALSEGGMERADIDTIMSRIASFEKVIVSKAES"
     gene            7143..8510
                     /locus_tag="PLG78_01490"
     CDS             7143..8510
                     /locus_tag="PLG78_01490"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21806.1"
                     /translation="MSWIQKIRNLLLLGDLLRGLGTEEARAVRVTNWIQIIFLTITTL
                     LWPQVLINEPPETHAVSSLLYVQIVLFMVIGRIFLYARRFYVSSIITLAGVFIHLGGL
                     CVAYADDPAHYGYFLLMLLPFLLIPSRHFWTRWAFVVIAGLGFLADQVYFRIMSGAAL
                     FGPPKSVLPADYLMPPMIMVVIFYALLINSFVKAVTTAETALAKEHAASERLLLNILP
                     EEIAAELKVKGASDPRYFEATTICFTDFAGFTQIAESMTPTDLVAELDKCFSYFDRVI
                     GRNGLEKLKTIGDSYMFAGGVPVPSPTHAIDSVMAALEIQAFMNQMKEIKSQQGLPYW
                     ELRLGIHSGDLVAGVIGERKFAYDVWSDTVNTASRCESSGVSGKINISKATYELVKDF
                     FQCEYRGPIPAKNKGTIDMYFVNGILPGLSRENEPRVPNQAFRDLYQRKVEEGGKAAG
                     KAAAG"
     gene            complement(8517..8589)
                     /locus_tag="PLG78_01495"
     tRNA            complement(8517..8589)
                     /locus_tag="PLG78_01495"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8554..8556),aa:Asn,seq:gtt)
     gene            complement(8675..9034)
                     /locus_tag="PLG78_01500"
     CDS             complement(8675..9034)
                     /locus_tag="PLG78_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21807.1"
                     /translation="MRFNKSLIAAFLGFQPGGWMIFDGFHVLTTGKYYGPPEPGPWAS
                     LVSALGFHPFSLGPAFLILGFAWVVAATLLLLKPAVGRILSLCLAVVTLFYLPVGTLY
                     AVCVIALLFRPETPAQD"
     gene            complement(9043..9459)
                     /locus_tag="PLG78_01505"
     CDS             complement(9043..9459)
                     /locus_tag="PLG78_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21808.1"
                     /translation="MKAKTLLKITAGLIGFFCLAHTAGAFLGKSHGAEEDALLSAMRS
                     YHFDAMGFSRTHWDFYFGFSLFLSVNLAALTRIIWVISSSSDANLVRTFSWVLTFTTV
                     GFAILSWVYFFPAPAIVNSLATITSLGAAIKSGNST"
     gene            complement(9459..9971)
                     /locus_tag="PLG78_01510"
     CDS             complement(9459..9971)
                     /locus_tag="PLG78_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNE21809.1"
                     /translation="MNFAEKERESFYSRLIWLFPAIFFFHVIEESLGFARWVQEILEG
                     QIDISAFYINNAGFMVVLLGLTALASRKKTMWATVPLFFWVSGQQFWNFIFHMYTQFH
                     FRHYSPGYFTALFLYFPVFLYLSYIALRERFLSGGFLFAALVAGAFGMWFTIWAGLYH
                     FGSFPWARWV"
     gene            complement(9968..10435)
                     /locus_tag="PLG78_01515"
     CDS             complement(9968..10435)
                     /locus_tag="PLG78_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21810.1"
                     /translation="MPSKRSFIHVCLLLFLVAWGCRQKDLPVLKVDAVHSQTHRPDKT
                     EAPPYEIGSAAGVILDSAEFQFPAFGKADARPNVVQIVGGEHAMYAARFDPSSKNELS
                     ADTLRPMKSSPEFSGFKAGDEFIIAIGDQLPPPSAGKPAPFRVMWIAMMKVKK"
     gene            complement(10487..11077)
                     /locus_tag="PLG78_01520"
     CDS             complement(10487..11077)
                     /locus_tag="PLG78_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE21811.1"
                     /translation="MKTICVFCGSSSAVSPQFYSAADAMAREILKRDLTLVYGGATVG
                     LMGQIAREVMAGGGRVIGVIPGFLASREIAHNGITELIHVNTMHERKARMESLSDCFA
                     ALPGGLGTIEEIFEMLTWVQLGVHNKPCGFLNTDGYYDPILAFLEKASNEKFLSASFR
                     EHIHFESSPEALMARLTGHTNRKEGSRQNQITPERM"
     gene            complement(11074..11766)
                     /locus_tag="PLG78_01525"
     CDS             complement(11074..11766)
                     /locus_tag="PLG78_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNE21812.1"
                     /translation="MGAFAFAEFRAGKLWLNMPSRSAYPVRGIDVSHHQGRIRWKLVA
                     QDQVQFAYIKATEGGDFVDPDFKRNWTEAAEAGLLRGAYHFFTLCRSGVEQARHFAKT
                     LPQEPQLPPVVDLEFTGNCNESQKVEDPTKEIEDFLTTLESLTGRKTAIYTTYDFSRT
                     YLNDRTFARDIWIRDVAFEPRGAFQNHFTIWQYTMYARVQGVRGRVDMNAFHGSMDDF
                     KSWANQPGPGPH"
     gene            complement(11811..12749)
                     /locus_tag="PLG78_01530"
     CDS             complement(11811..12749)
                     /locus_tag="PLG78_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE21813.1"
                     /translation="MRSNSLYWIVLFFVGLLARSAQAENGPLRIDSLKDCSSVHTGFF
                     VYYPVESMPIIIERGERTQIQHRVFGKFYKIERIDWTASCSYSLRTIEVHDPLLQGEK
                     QSPSTIEVTAIHENSYEYKELTNGKEETGTIQFARDLDSIKKKQLASPINQSVGDEDL
                     LKACQTGRASEIQKLLNQGANPNVKDKNGFTCLMMQAVQGSLPAVKALVMANADVNAV
                     QESCGCTAFLYAAHAGKAEVADYLARHGANISAEDKDGDNALIPPASHGYMRTVRLLL
                     TLKIDRNHKNRAGKTALDSARENKQTEVVKLLEETP"
     gene            complement(12769..13443)
                     /locus_tag="PLG78_01535"
     CDS             complement(12769..13443)
                     /locus_tag="PLG78_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE21814.1"
                     /translation="MKARGTLIVFDIDGTLTDTAGLHGKFIAQCLLDSGVSSFNSDWN
                     SYAHHTDSHILRHIYEIQNGRAFSKETIRSYDLLLERRYRELHKHVPISQITGATDLL
                     TALAQYEIPFCFATGGLRKSAELKLIAAGIPFDPSQISAANDFETRIEIVRDSVRRRA
                     EKSAHPVSRIISVGDGPWDLETAQEAGIDFVGVTMNATPPSFCSSRPVICSGNQESLL
                     KLLDYL"
     gene            complement(13440..>14708)
                     /locus_tag="PLG78_01540"
     CDS             complement(13440..>14708)
                     /locus_tag="PLG78_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21815.1"
                     /translation="ASETSPLHFANTAKFIEETSLKSQKLDSARLSSRHADGDIVQCF
                     LRMRIKHSFFYYRAIFQFSLREEVSIFFEIVRPTFRPLFLSLIFALTQALHAAPDKDA
                     KKDDSGFYTFGLQLMFWTGLNRDFYSSDLETRFFRDDILRGQNTAQPVAAAQYNNKWV
                     SKPQFNLPVRGTFSPGFLPQLNLEGAYYRERGNITYYGLSDLPAPDRTHIVELSGFDR
                     STTTFGAAYNFLGYFRVDPMSLLALRGGYIRESTDFNFKPLSFATSGVIVSLFDNPFH
                     AVASGWYGGIELTLPIWQKWSATLKVDRSEALHGNMQYEKIQLQPASGGIQATYDRAE
                     SRYRLARTDRMAAVNYHITSDFKVTVGYREVIYDVSYPGFFSYSLTNAGGAVQVFPVT
                     EFILDYFNYQETRRERIGNAMFAFEKIFVF"
BASE COUNT         3529 a         3595 c         3835 g         3749 t
ORIGIN      
        1 aggcatttct ccgcgcgtta cctgaactgt gctccatccg cccaggtatg tataaaaacc
       61 caggacaaca acggccagcg cggcgatcag agtgagtagg atcttgactg ctttcattgg
      121 acaattcgta gagagcgtgg aaagttcgtc aaccatgcca tgcgcgttgc ttgacttttt
      181 cgcaccggtc agttccattt gtgcgaaatt caggtttcgc cgaatgtttg cttttttgta
      241 ctttgtgcgt cgcttttgct tctatggctc cgccttcttt ttctgttcgt gcgtcgcggg
      301 ttgcaggttt tctgccggtg ctccggagtc tatctggcgg gccctctttg tttacgatgt
      361 agcatttctt gacctttgcg gtccgcaggt atccgaggtg aacgtgctca gttcagacaa
      421 tgcagcgcga gtccggctct actttcagtt catgcaatct ctcggaagca ttcgttcccc
      481 ttctattaca acttttggct ccgtgggagc caatacatac cagatgggat gcctggctcg
      541 aaatgggaag atatacgcaa tgccggataa cgctacccag gttctggaaa tagatccaga
      601 taccagaacg gtatccaatg ttcctggaac tgtggcggga gccaatccga agtattctgg
      661 atgtgcgctg caggcgaaca acggaaagat ctggggagct gcgcgaaatc caacgcaaat
      721 tctggaagtg gacccgacta ctcgtagtac ttttcaggac attgccggaa gtttaccctt
      781 cgcaagtttt ctgggcgcca aaactggacc ggacggtcac atatacgctt tccgagattc
      841 tgctggagca gttctggatt acaatccgaa tactagaact tccacaagct ttgcggccat
      901 ccccgggtat ggagaagctg tgcttgcaac caacggtaaa ttttacggag ttccaaacaa
      961 cacgaatccc cctgtgggag aaatcgatcc cattgccagg tctcttcgaa ccggtggagg
     1021 cacgtatggt gtaggcgctt atgacggagg atctattgcg cctaacggta aggtctacat
     1081 gacacctctg gctgcaacca ctgttctgga agtggatccg gatctatttc cagcatcgaa
     1141 tgcattcagt cagtttggca gcacatttgg aaaccagtat tccaatggaa ttctggcacc
     1201 gaatggaaag atctatgctc cgccgcggac aggcacttct gttctggtta cagatccgtt
     1261 gaatagaact acctctacga tcggcactac ttcgggggtt tacattggtg ccaccatggg
     1321 cttgaacgga aagatctact tcattccaaa cggagccacc accatcgctg aactggacac
     1381 aggttcggct ggcacctttt gtccgaatgt gattttatct tcttatataa attccaacta
     1441 gtatagatca ctcccgctgc gcgtaaacca gtccagaatt tccgcattca ggtcgcgtgg
     1501 atagttgtgc gaaaggtctt ccacttcgcg gtagacgatg tttgctcctg cattcttgag
     1561 cgctgatgcg gcgagtcttg ctttttccac agggaacatc cagtcgcgcg ctccatgcac
     1621 ctgaaagatt ttcaggtctg cgaccggcgc ttcgacgatt ccttctctgt tcaaaagagc
     1681 gtgtactgct gcggctacag gagcatagtg ggtgaaagga gattgtctga cagtggagag
     1741 gaccatggag tatgttccgc cgtctgaaat ccccgtgagc aagaggcgct ttgtatccac
     1801 gttccatttt tctgagatct gactcagcat ggaattcagg cccactacat ccgtgccagg
     1861 atggtggaga aaccaggtgc gatccagaga tgtaggggac gcaaggagca tcccgcgtgc
     1921 gcgcgcatct cgcagccagc tccacaggaa atcagctccg tgaccggatc caccgtgcag
     1981 tgcaatgacc agaggccaac gtttttcttt tgtatagtat tctggcacat agatagacca
     2041 tccgcctctc tttccgcgtt cgttgttgaa atggatgatt ccacagtcac tcgcatccga
     2101 aagtgacgca atgtcgcctg acggtgactg agacaacctt ttcagcagct ctcgattttc
     2161 tctgtgcgat gctggaagaa agaatttgct cacttcttca aattgacccg ccagcgaata
     2221 caggatttcc tccgagcgat ggatgggtct aagcgcctgg aatgccttca tgattccgtg
     2281 cgggcctgcg ggagcttcca ggaatcgagc ggctccctgc aatgcctggg aagcggaacg
     2341 caacaatacg tctacggatg gagcgttctg ggtgctacgc tctgcttcat ttctagaaag
     2401 ttccagtttt gcggccagtg gttcaattct gcttgcagat tgtgaaaaga gggttggatc
     2461 cagcctgtcc tggatttcgc caaaagctgt gagcagttcc gaaaggtctt tccacaaagc
     2521 atccattggt tttggatttc gacgagctct gctgagtgcg tcaacctcaa acaaaacagc
     2581 tcggcaaata aacagatatt gccttgctgc gtgcttgcac cgccctctgg tcgcaatcag
     2641 gatggttcta tggaagcagt ggagactcga attcttcttt ttgatgatgt ggaagttttg
     2701 gattttgcgg gaccgtttga agtcttctcc atcgcggaga ctccggatca cagaaaagcg
     2761 tttaacgtta aaattgtagc ggaaagcaaa cgcactgtcg ttgccaggaa tggtctaaaa
     2821 atcgtgccag attcgagtga aacagggcgc tcgttttgtg gatcagggaa gaatcatcac
     2881 gtccgccgga atctcagccg gaattgatat gtctcttcat atagtagaaa gatactttgg
     2941 aaaagaagct tcacgcgcga cagcgcggag aatggaatac gattttataa gttgaaagcg
     3001 ggcgttgagc gccctcacac tccgcgcggc aagatcacag gctttccaga cgtcatattt
     3061 tctgcgtatg ccgcaattcc ttccggtgcc tgcgccagag tgagccgcgc ggaaaactcg
     3121 gtatgaaaga tttttctctc tacgagtttt tgaacctgcc gtgccgtttt tagaacccag
     3181 agtgttccgc gttccttgat gtgcgaagca agatgaaagc ccgtcacatt ctttctgaaa
     3241 aacacaatgt cttttgattt cgcagtcgca tcttctccgg acatagaacc atacactatg
     3301 atttgagaac gagaaggaag agcctgtaga acgcgtgcag acatggcacc gctgatgcaa
     3361 tcgaatgcgg tggttgcctt caggtcctgg gcgagtttct ggagttgtgc gtcaaaatcg
     3421 ttttcgctgg agttgagtac atgagcattt ccggcattct tcaagagttc cacgtgttct
     3481 tttttgcgga caatgttgat gagcggaatt ccttgctcgc gggcaagtgc tgcaatcatt
     3541 ctgcccagct ggcttgcggc tgcatcctga acgagagcct tgctcccgaa ttctctcgca
     3601 cgttcgagga gagcgaatgc agtgagaggg ttcacaatca gcgaacaacc ttgctcaaag
     3661 ctcacgccac ggcgaagcgg aagacaggac attgcatctg caatgaagta ttctgcccat
     3721 gtgccatcgc gtggactctg gccggcgcac gttatgcgct ttcccaggag ccaggatcca
     3781 ataagcccac cgcccgattt cacaaccaca ccggaagctt cgaagccagg taccgcgggc
     3841 aggggccggt ctactgcgta atgaccctgg agataggaga gatcggacgg attgcacggc
     3901 gcggcttcca ctcgcacaag aacctgtcct ttcgttggaa ctggcactgg cttgcgcaca
     3961 actttcaaat tggcacaagc ttcgcgcaca gtggatccgt ttgcgctcag ttccagcgct
     4021 tgcatctcgc gtggtatttc gaaacgactc atctgctgcc tccctattcc acaccgcgcc
     4081 agcgatcgac ggccgcgccc tgcggcccga gagcgcggtc cgcgaagcgg aggcgcccaa
     4141 tcacttcccc tctgtctgat ccattcttct tgcgagcacc aggctcgaaa tgccaggctt
     4201 ccaggcggcc gtgtagccaa tgttaaatgg tagttccttt acgtctttct tgtttgaata
     4261 cgcacgaagc atatcatact ggatttgtcc tgcaaactga ggaatgggtg cctgataatt
     4321 tccgtagagc gtgagattcc aggttttgcg atcccaatct ttgaacggga ttcctgtatc
     4381 gtcggataga atgaggcgcg attcattcag gataaaagcc ttgagagttg gaaaattgcc
     4441 ccagtgaaga aggtaggacg cggctttcag catgactgac ctttcacccc tgcttttcac
     4501 aaaatcaaaa aagtaggatc tcgttttcat agccgggtct gaggcatcga atgagaagta
     4561 gtaggcctgt ctttccgttc cgttctttac gaacgttatg cgcgcaccgc gaatgcccgt
     4621 tgtctttatg gaggattccg gaatccgacc gggtgcaggc gaattttctt ccagttcgta
     4681 gagtgttcca ttgtttccaa gtatgacacc ggtgacgtcc gttacgcgat ttcctgttcg
     4741 cgcaagaaac acaagcatga gtggcagggt tcccgaaagg tccgtgtatc tcagctggct
     4801 gtccatgtga atcgtctgaa agtatgtata gctcgtcagc catttgagcg actcacgaag
     4861 attggtgaga acaggattca accgggcagc cgttactttc gttggatcgg gtggaaggcc
     4921 cggcgcttcc aggccgcaca tgatgtattc acgcacatga ggaaaaaagg tttgagatat
     4981 gatgatctcc ggtccaccga acggataaaa tagaactggc gttcccggat tgccgatctc
     5041 tcgctctgcc cagggcttca tcttcgaaag atagttctct tccaggccag agaacatttt
     5101 gtccatggat tgtttgtgag tgacccattc cggtctgcga gatagctgag cgaggttcgg
     5161 gtcttcctgg acttccatgc ccgcaagata tcgcgccaca gaatcccagt ggccagtatc
     5221 gcgtggatgg gcgggcgtgc aggaggcggc cgcaagaagg agaaaggtaa aggttagaat
     5281 ttttttcata ggtgatctgt tcagaagcga acattcgtgg agtatttgca cctggtttgc
     5341 ggcacttcta ttcttcgcac acaaaaaaaa tgggcccgaa acgccggctt ttttttgagg
     5401 aatggcgcgc cagcggccaa tgtgatctgg cacccagaac gactcccgtt tagctccaac
     5461 acttgtgggc cgcttccatc gtttgtgaag cttcgcgttg taaggcgaac cattgtgact
     5521 gcacagttaa tgcatttaca gaaaagcaga gagtcgattt ttcattccag cacatgtcat
     5581 gtgtgaccgg attggagtat gtggaacagg atacattcgg acgcctttcc tggatcatcc
     5641 ccattgcagc agtcatgatg ctcgcggact ttctgttctt tgctctcgta tactccagtc
     5701 ccggcgggaa tctaatcacg gcacaggtgg gtcttgcatc ttctgtgatc gctctgactc
     5761 tgagcactgc acttctctat cttttgcgaa ccatagtgga cggggcgatc ctcagagtgg
     5821 aagccctgaa tctttctctt tctcgatttg ttccagctga attcttgcgt ttgttgaatc
     5881 gccagagtgt ggagcaaatt tcactcggag aaagcaaaga agccacgatg agtgttctgt
     5941 tctctgacat tcgcgatttt actgcaatcg cggaagggaa ctctccagag aacaatttca
     6001 aattcataaa cgattatctg aaacgaatgg gacccgtcat acgcgatcac aatgggttca
     6061 ttgataaata cattggagac ggaattctgg ccttgttcga acatgcagac gacgcagtgc
     6121 gggcagggct cgctatgatc gcggaactag aaaatttcaa tcgtgagcga tcagaaccgg
     6181 tggaaatcgg aatcggcatt cacacaggga cgcatcatgc tcggcacaat cggagaagag
     6241 cttcgcatgg aaaacactgt gatcggagac acggtcaact cagcatcgcg cattgaggga
     6301 cttacaaagg tgtattacac aaggtttctc gtgagccggg acactctgag tgcaatgcag
     6361 gatcacacag ggattcgttc acgcctggtg gacatcactc ttgtgaaagg aaagcgcaag
     6421 cctttatacg tacacgaagt gatctgcgga gaagaggagt ccatcatggc tgcgagatac
     6481 tcaggcgggg cgttgcttga aaagggagtg catgcagttc gatccggaga ttacatttct
     6541 ggaaagaata tcttcgaaga gtatctttcg caatatccat ttgaccgcac tgcgaaagtt
     6601 catttacaga gatgcgctcg cgtattcaat caattcaaag gaaaatcgat ctttgataag
     6661 tatggagata tttctactgt aggaagtctg gtggagctct tctactctaa gatcttagag
     6721 gatgatttgc tcagcgactt tttcacgaac acgaacatgg tgcgtctgaa gggacaccag
     6781 actcgattca tcgccatgct tatgggcgca cccattcgat ttgacacttg cgctctggaa
     6841 tacgcgcacg ctcatttgcc catcacgaga gctcattttc agagggtggg cctccttctc
     6901 gagaacgcac tttctgaagg aggaatggag agggcggaca ttgatacaat catgtcgcgc
     6961 attgcaagtt ttgaaaaggt gatcgtttca aaagcggagt cctgacagct tgctaaccat
     7021 ggcgacctca atgagtataa cagcagtttt gcgattgttt gcgcaaaaca ggattgagcc
     7081 tcgcgcgcgc aggcgcccat aaatgtgatt gcctcgcttt tttcatagag gcgaatgcac
     7141 ttatgtcttg gattcaaaag attcgcaact tattgctgtt aggggatcta ctccgggggc
     7201 ttggcacaga ggaagcgcgc gcggttcgcg tcactaactg gattcaaatc atcttcctta
     7261 caatcactac tctgttgtgg cctcaggttc ttataaatga acctccggag actcacgcag
     7321 tgagttctct tttgtatgtt cagatcgtct tgttcatggt gattggtcgg atttttctct
     7381 atgcacgccg attttatgtg agctccatca tcactcttgc aggcgtattc atccatctag
     7441 ggggactttg cgtggcctat gcggatgatc cagcgcacta tggatatttt cttttgatgc
     7501 tgctgccgtt tttgctgatc ccatcccggc atttttggac gcgctgggct tttgttgtga
     7561 tcgcgggtct cgggttcctg gcggatcagg tctactttcg gatcatgagc ggcgcggctc
     7621 tctttggccc gcctaaatcc gtgctcccgg cggactatct catgcctccc atgatcatgg
     7681 ttgtaatttt ctacgcgctg ctcatcaatt cgtttgtcaa agcagtcacc acggcagaga
     7741 ccgctctggc aaaagaacat gctgcttccg aaagactctt gctcaatatt ttgccggagg
     7801 aaattgccgc agaattgaaa gtgaaaggag caagcgatcc cagatatttt gaagcgacca
     7861 ccatctgctt caccgatttc gcgggcttca ctcaaatcgc agaatcgatg acaccaaccg
     7921 atctggtggc ggagctcgat aaatgttttt cctatttcga tagagtgatc ggacgcaatg
     7981 gactggaaaa attgaaaacg ataggcgata gctacatgtt cgccggcgga gttcccgttc
     8041 cttcgccgac gcatgcgatc gactcggtta tggctgctct cgaaatccag gcattcatga
     8101 atcagatgaa ggaaatcaaa agccagcaag gtcttcctta ctgggaactg cggcttggaa
     8161 ttcattcggg agatctggtg gcaggtgtga ttggagaacg aaaatttgcg tacgatgtat
     8221 ggagcgacac tgtaaacact gcgagtcgct gcgaatcctc cggagtttcg ggtaagatca
     8281 atatttcgaa agcgacctat gagctcgtaa aagatttttt tcagtgcgaa tatcgcgggc
     8341 cgattccggc gaaaaacaaa ggaacgatcg acatgtattt cgtaaacggg attcttccag
     8401 ggctttcgcg cgagaatgaa cctagagtgc ccaaccaggc gtttcgggat ctttatcaga
     8461 gaaaggtgga ggaagggggg aaggcagccg gaaaagctgc ggctggttga agaacgctcc
     8521 cccgcctggg ctcgaaccag ggacctagtg attaacagtc acgcgctcta ccgactgagc
     8581 tacaggggat tgttgcggcc atgttcgatc gcaggcgcct ccgggtcaat tacaaatgcc
     8641 tgggaccccg gcttccgcgc cagattccgg ggagttagtc ctgcgcgggt gtttccgggc
     8701 gaaagagaag cgcaatcaca cagaccgcgt agagagtccc gactggcaga taaaaaagag
     8761 tcaccacggc cagacagaga gaaaggattc tgcccaccgc cggtttcaaa aggagcagag
     8821 tcgcagccac cacccatgcg aatccgagga tcaaaaacgc aggacccagg ctaaagggat
     8881 ggaaacccag ggcagagaca aggcttgccc aggggcctgg ttctggtggg ccatagtatt
     8941 tgcctgtggt cagtacgtga aagccgtcaa agatcatcca gccgcctggc tggaatccta
     9001 gaaatgcagc aatgagagat ttattgaatc gcataatcgt tgttaggtgg agtttccgct
     9061 cttgatagcc gcgccaagcg acgtgattgt ggccagtgaa tttacaatcg ccggagccgg
     9121 aaagaaatat acccaggata gaattgcgaa tccgaccgtt gtgaacgtca aaacccagct
     9181 aaaggttctg accaggtttg cgtccgatga agaagatatg acccagatga ttctagtcag
     9241 tgcggccagg tttacgctca gaaacaggct aaatccaaaa tagaaatccc agtgagttct
     9301 ggaaaatccc atggcgtcaa aatgatacga cctcatcgcg gagaggagag cgtcttcctc
     9361 cgcaccgtga gattttccaa gaaaagctcc ggcagtatgc gcgagacaga aaaaaccaat
     9421 cagtcctgcg gtgattttta aaagagtctt cgctttcatt atacccacct ggcccaggga
     9481 aaagatccaa agtgatagag cccggcccag atcgtaaacc acattccaaa agcaccggcc
     9541 acgagagccg cgaataagaa tcctccgctg aggaaccttt cgcgcagtgc gatgtagctt
     9601 agatacagaa atacagggaa gtacagaaac agagccgtaa aatatccagg agagtagtgg
     9661 cgaaagtgaa actgggtgta catatgaaag atgaagttcc agaattgctg tccgcttacc
     9721 cagaagaaca gcggaaccgt ggcccacatt gtctttttgc gcgaagcaag tgcagtcaat
     9781 ccaagtagaa ccaccataaa gcctgcgttg ttgatataaa acgcgctgat gtctatctgc
     9841 ccttccagga tttcctggac ccatcgcgcg aatcccagag actcttctat tacatgaaaa
     9901 aagaagatcg cagggaaaag ccagatcaat ctggagtaga aactctctct ttctttttct
     9961 gcgaagttca tttcttcacc ttcatcatcg caatccacat cactcgaaag ggtgctggtt
    10021 ttcctgcaga tggaggagga agctgatcgc ctatggcgat gatgaattca tctccggctt
    10081 tgaatccgct gaactccggg gagcttttca tcgggcgtag agtgtctgcg gacagttcat
    10141 ttttggaaga ggggtcgaat cgcgccgcat acatggcatg ttctccgccc acgatttgca
    10201 ctacgtttgg ccttgcgtca gccttgccga atgccggaaa ttgaaattcg gcagaatcga
    10261 gtatgactcc ggccgcgctg ccgatctcgt aaggaggagc ttccgtttta tccggtctgt
    10321 gcgtctgaga atggactgcg tccactttga gaacaggaag atctttttgt ctgcaacccc
    10381 aggcgaccag gaacaggagc aagcatacgt ggatgaatga tctcttggaa ggcatgatgg
    10441 aagatcgggc acggtccgca ctcgtcaagt ggtctgcgaa aatccgttac attctctcag
    10501 gcgtgatctg attctgacgg ctgccttctt tgcgattcgt atggccagtg agtctggcca
    10561 tgagagcctc ggggcttgat tcaaagtgaa tgtgctccct gaaactggcg gacaagaact
    10621 tctcattgga ggctttttca agaaacgcaa ggataggatc gtagtagccg tccgtgttca
    10681 aaaaaccgca tggcttgttg tgtactccga gctgcaccca ggttagcatt tcaaagattt
    10741 cctcaatcgt gccaagacct cccgggagtg cagcaaagca atcggacagc gattccatac
    10801 gtgcttttct ttcgtgcatt gtgtttacgt gaatgagctc cgtgataccg ttatgcgcta
    10861 tttctctgga tgcaagaaat ccagggatca ctccaatgac tcgtccaccg ccggccatca
    10921 cttcgcgtgc gatctgtccc atgagtccga cggttgcgcc tccgtatacc agcgttaaat
    10981 ctcttttcag aatttcgcgt gccattgcat ctgcagcgga gtagaactga ggcgacacgg
    11041 cagaacttga accgcaaaat acgcaaattg ttttcaatgt ggccccggtc cgggttgatt
    11101 tgcccaggat ttgaaatcgt ccatggagcc gtgaaatgca ttcatgtcca ctctaccgcg
    11161 cactccctgg actcgagcat acatggtata ctgccagatt gtaaaatgat tttgaaaagc
    11221 gccgcgcggt tcaaaggcaa cgtctcgaat ccagatatct cgcgcgaatg tgcgatcgtt
    11281 gaggtaggtt cgcgaaaaat cataggttgt gtagatagct gtctttcttc ctgtaagtga
    11341 ttcaagagta gtgaggaaat cttctatctc tttcgtgggg tcctctacct tttgcgattc
    11401 gttgcagttt cctgtgaatt ccaggtccac aaccggtggc agttgaggct cctgcggcag
    11461 ggttttcgca aagtgcctgg cctgttctac tccagatctg catagagtga agaaatggta
    11521 agcgccgcga aggagtcccg cttctgccgc ctctgtccag tttctcttga aatccggatc
    11581 tacaaaatct ccgccttccg ttgctttgat gtaagcgaat tgcacctggt cctgagccac
    11641 cagtttccag cggatcctgc cttgatgatg agatacgtca attccgcgaa cgggataggc
    11701 agatctagaa ggcatattga gccagagttt accagcgcga aactccgcga aggcgaacgc
    11761 tcccacaaca atcaatgcaa gagcgatgca tcccccgaga atcgaaatct tcagggggtt
    11821 tcctccagaa gtttgactac ttccgtttgc ttgttttcgc gagcagaatc gagagctgtt
    11881 ttgcccgcgc gatttttatg attcctgtct attttcagag tgagtagaag acggaccgtc
    11941 ctcatatatc catgtgatgc tggcggaatc agtgcgttgt ctccgtcttt gtcttccgcg
    12001 gatatgttgg caccatgtcg ggccaggtaa tctgcgactt ctgctttgcc cgcgtgcgcg
    12061 gcatagagga aggcagtgca tccgcaagat tcctgtactg cgtttacgtc tgcgttggcc
    12121 atcacgagcg ctttcaccgc aggaagagat ccttgcactg cctgcatcat caggcatgtg
    12181 aagccattct tatctttgac gttcgggttg gccccctgat tgagaagttt ctgaatttca
    12241 gaagcacgac ccgtctgaca cgccttcaat agatcctcgt ctcccacgga ttgattgatc
    12301 ggacttgcca gctgtttctt ttttatggaa tccagatctc gcgcaaattg aatggtccct
    12361 gtttcttctt ttccgttcgt aagctctttg tattcgtatg agttttcgtg gatggcagtg
    12421 acttcgatgg tggatggaga ttgtttctcc ccctggagaa gtggatcgtg cacttctatg
    12481 gttctcaaag aataagagca actcgccgtc caatctattc gttctatttt ataaaatttc
    12541 ccaaatactc tgtgctggat ttgcgttctt tctcctcttt caatgatgat gggcatgctt
    12601 tccaccgggt agtatacaaa gaatccagtg tgcactgatg agcagtcttt gagtgaatcg
    12661 attcgcagcg gcccgttttc tgcctgcgcg ctccgggcaa gcaagccgac gaagaaaagg
    12721 acgatccagt agagagagtt tgatcgcatg atcaaggaga gtctttgctt acaaatagtc
    12781 cagcaacttc aagaggcttt cttgatttcc agagcagatc acaggacgac tggaacagaa
    12841 cgaaggcgga gttgcattca ttgtcacccc cacaaaatcg attcccgcct cctgtgcggt
    12901 ctctagatcc cacgggccat ctcccacaga aatgattctg gaaacgggat gtgccgattt
    12961 ctccgctctt cgacggactg aatctcttac gatttcaatt ctggtttcaa aatcattggc
    13021 cgcactgatc tgagagggat caaatggaat tccggctgct atcagtttca gctccgccga
    13081 ttttctcagg ccccctgtcg caaaacagaa aggaatttca tactgcgcaa gagcagtgag
    13141 caggtctgtg gcgcctgtga tctgtgagat tgggacgtgt ttgtgcagtt cgcgatatcg
    13201 tctctcgagg agtaagtcat aggagcggat ggtttctttc gaaaacgctc gaccattctg
    13261 gatttcgtaa atgtgtctga gaatgtgaga atctgtatga tgtgcgtacg agttccagtc
    13321 tgaattgaaa ctggaaaccc cgctgtccaa aaggcactgg gcgatgaatt tgccgtgcag
    13381 ccccgcagta tctgtgagag tgccgtcaat gtcaaagacg atcagtgttc cccgtgcttt
    13441 caaaatacaa aaatcttttc aaatgcgaac atggcgtttc caattctctc tcgcctggtt
    13501 tcctgatagt tgaagtagtc cagaatgaat tctgtaactg gaaatacctg gactgcaccg
    13561 cctgcgtttg tgagagaata gctgaagaag cccgggtaac tcacgtcgta gattacttct
    13621 ctgtaaccca cggtcacttt gaagtcagac gttatgtggt agttgacggc ggccattcta
    13681 tctgttcgcg caaggcgata tcgcgactca gctcggtcgt aggttgcctg aatcccaccg
    13741 cttgcaggtt ggagctggat cttttcgtat tgcatattgc cgtgaagcgc ttcgcttcta
    13801 tccactttca gagtggcgga ccatttctgc catatgggga gagttaactc aatgccacca
    13861 taccatccgg atgcgactgc gtggaatgga ttgtcaaaga gggaaacaat gacgcctgac
    13921 gttgcaaaag aaagaggttt gaaattgaag tcagtgcttt ctcgaatgta tcctccgcga
    13981 agggcaagaa gggacatcgg gtccactcta aagtaaccta aaaaattata cgcggcgcca
    14041 aacgtggtgg tggacctgtc aaaaccgctg agttctacga tgtgagttcg gtcaggcgcg
    14101 ggcagatccg acaatccgta gtacgtaata ttcccgcgct cgcggtagta ggctccttcg
    14161 aggttcaact ggggaagaaa tccaggagag aatgttcctc gcacagggag gttgaactgt
    14221 ggtttgctta cccatttgtt gttgtactgt gccgctgcca ctggctgggc ggtgttttgg
    14281 ccacgcaaga tatcatcgcg aaagaagcgc gtttcgagat ccgagctgta gaaatctcga
    14341 ttgaggccgg tccagaacat gagctgaagg ccaaacgtat aaaagccaga atcatccttc
    14401 ttcgcgtctt tgtccggggc tgcgtgcagt gcctgggtaa gggcgaaaat gagggaaagg
    14461 aagagtggtc tgaatgtggg tcgcacaatc tcaaaaaaga tggaaacctc ttcgcgcaag
    14521 gaaaattgaa agatcgcgcg atagtaaaaa aacgaatgct tgattctcat tcgcaagaag
    14581 cattgcacaa tgtccccatc tgcatggcgt gaactcaatc gggctgaatc cagtttctgg
    14641 ctcttgagcg aagtctcctc tatgaacttc gctgtatttg cgaaatgcaa aggggaagtt
    14701 tctgatgc
//
LOCUS       DAOQJL010000019        14492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2007_length_14492_cov_4.557318, whole genome shotgun sequence.
ACCESSION   DAOQJL010000019 DAOQJL010000000
VERSION     DAOQJL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2007_length_14492_cov_4.557318"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="PLG78_01545"
     CDS             <1..1649
                     /locus_tag="PLG78_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNE21816.1"
                     /translation="SSLCILAILLFYNRLEGIPRTVLILDSVLVFLLLGFRSFSWRLF
                     RDIYFVRRARGGRRTLLIGAGVTANRLVTDLRTNESLQIMPIGYLDDDPAKGGAHLQG
                     LPVLGKIEDLDHVLTEKRIAEVIISAKLPGSVIRHIYRTCETRHIPCKTLPPLSELLA
                     RPDIGKSIRNVSLEDLLGRDVVRLETEGIRKAIENKVVLITGAGGSIGSEVCRQILTY
                     SPGVIVLFENAETPLYEIDYELRKLCLENNLRTHIDAVIGDVRNVEDLAQVFRQNEIN
                     TVFHCAAYKHVPMMELHPEEAVKNNVLGTINVANAARDAGVERFVMISTDKAVNPVNV
                     MGATKRIAEIYIQNLARVTDTRYITVRFGNVLGSNGSVIPLFQKQIEAGGPVTVTHPD
                     IIRYFMTIKEAAGLVIQAGVMGQGGEIFLLDMGEPVKIKDLAEDLIRFAGLVPHQDIQ
                     IHYIGLRPGEKLYEELLLAEEGIEPTRHEKIHIANSRISHLPTLLEKVHELEEAARRG
                     SRPDMDAIMSSIVPEYRPVSPMIRPADRAQEKENARSRKI"
     gene            complement(1675..2766)
                     /locus_tag="PLG78_01550"
     CDS             complement(1675..2766)
                     /locus_tag="PLG78_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21817.1"
                     /translation="MSRSSLVFLILASAALHVAALAIPAFKAGHLSAAVRFGCLSPTI
                     CYAMKNKAILPENAVVYKSGGYDGQFYFYSAMRVFRGVQVEFDSAPFRLARIGYPVLA
                     GMGALAGPGYLLYSMYFIPLALHLFVVFFLRSDPLRASIFAFNPISVLSAGLFLADGM
                     ALSLLVLALELAFKEKHSLVVLIIAALSMLCKETAAAGALSALFLFLRFRKPVFVALV
                     VACLPMIVWWFATGFSPFFAATRGTAGGPGVWNYVQHPDALLSGRSLLFLFYILGCIV
                     LFRLDLKQERFLAGAALIACGFLLSLTASSEYWDNFANVARLFLPVCAGLLLAARQWK
                     GRIVSGTFLFFSLLLLFREAKSVYDIEVW"
     gene            complement(2763..3143)
                     /locus_tag="PLG78_01555"
     CDS             complement(2763..3143)
                     /locus_tag="PLG78_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21818.1"
                     /translation="MANVRKPACPLDPASVKELFAVEVAAVDCSQWKPVFFEGVREIL
                     KSVEKQKSQTILYSVPPDLALQLSLCGVSVTEDSAVIQPDCLSRGIGQCPFCGAAIRF
                     EKAGRHQCPSCQNLLMVEDGAISA"
     gene            3198..4010
                     /locus_tag="PLG78_01560"
     CDS             3198..4010
                     /locus_tag="PLG78_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21819.1"
                     /translation="MAGVNKARLIWTWTQEAVWLALLVLQLPFVFVQKWSSHGRRKEP
                     VLLLTDLGLSVFAYFRIRMALARAGFPVIVLSIGSVSSLSSQARQLARTLEQLNIQKG
                     ILVAHGRGGLTALALPDSGRRRIQHLVTLGTPFHGSRFFTPFAFVPAFGDMNVGSDFL
                     LLHRMNALLFPGFSPFSAYKDEFIYPGNLGHFGQGRDLILDRNGHMNLILDGENIETI
                     VQHIESLHPEPAANPEVRAAKPPEVAPPAAKKSAKKSSKKKPSRPGRKKGKR"
     gene            4007..4309
                     /locus_tag="PLG78_01565"
     CDS             4007..4309
                     /locus_tag="PLG78_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNE21820.1"
                     /translation="MNLKEIQRVIDDWIGKNGGYYSELTNLARLTEELGELARLYARR
                     EIPARDLEDVSRHKMQEEMGDIVFALCVLANQAGIDLETSVQDVLNKVKLRDADRH"
     gene            4347..4745
                     /locus_tag="PLG78_01570"
     CDS             4347..4745
                     /locus_tag="PLG78_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21821.1"
                     /translation="MRAILASIACLLLVQCAGANAGIAVSNVPTEGQTYTVLGTAETT
                     KSWWSLDLAVLAFPLGEPPVAEAVEDLLKQKEGNALINLRFWTDRTTILFMTRHRFHL
                     QADVIRFDPKVDPDKDKKDKAKDDKDKLKK"
     gene            4742..5326
                     /locus_tag="PLG78_01575"
     CDS             4742..5326
                     /locus_tag="PLG78_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21822.1"
                     /translation="MKRFLFIAILAAGACTPGGLIDKGGYRDQGGIQPQASRTHGVAY
                     KILCYDSQEARRREGLPEGAEKPPGPPVRIEDPATYKGGCEEEGESSVFFLFNIVPVT
                     PPLNPEYAIGTAVQKLEGDTMIGMKTWQETHYYSILGRVSVFKVKGLVIKFDNKEPTD
                     SKSDSKDKDKKGDKATDKKDDKTDKKDLKADQKK"
     gene            5323..5610
                     /locus_tag="PLG78_01580"
     CDS             5323..5610
                     /locus_tag="PLG78_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21823.1"
                     /translation="MKVIAFAVLLLLSGCIVENDTVDTADAYSEIYASIQFKARQCGN
                     QPAYPLLVPENPPSYGTRLCSLSIIRQDCPFTNYPLFCVEMFGIDLPGIGP"
     gene            5610..8099
                     /locus_tag="PLG78_01585"
     CDS             5610..8099
                     /locus_tag="PLG78_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE21824.1"
                     /translation="MKWRALLVFFLLFPATVFAIDVKIKIEDKKAGKPVSDAQVIVME
                     TKEKFFSDETGEAIIRIPAKGFYTIRVISPSGEIVQPRVEVISPNQTVTIFLGIEEEK
                     PIPTNTPQKVTAEEGISVTGKRERQKVSRYQVRIDEVKRIPGQFGEALRGLETLPGVN
                     APPFGNGDIAVRGANTNSNTYLLDDLPIGYAFHFAGLNSVIHNDLVKSIDLFTGAYPV
                     TYGNATGGVVAIDTIDEVERFGGHATFSLWSAQALFKGKIGDGYWIGSGRMSYLDITL
                     RPYIPEGTQFIPRYWDGQFKAMQRLSQTQKLYVYALGAKDTFIAKIDNKPSWDPTAEF
                     DPRFVGAQIALDQAFHTEAIKHVWNPGTRINNELTLYYHNNIQYIDGQLGLLRPKQKR
                     ESGWGAFKNEFDWEIYKEHLFLDVGLEARRYEYQNNGQATYLVNPNNPSPDPYKSVNP
                     DFVNLPVHDSQMTSYNTGYTMLTLKAWGFEAKPGVRVDYFGLTKQRVADPRGTLSYTA
                     PWKMTISAGGGVYHRLPDAETYSRSSGNPNLKMERAEHYGGGIEQIIESWTLKAEAYR
                     HYFTDIVVADPYITTPVRLNQDPFNRYTTPYIFNAPLNFSNDGTGFSEGWELYIKKSK
                     PDNKNGLYGWISYTWSRTLRNNHQHIITDDEKRQIYSADEARIIHEYDNTKDHYADFD
                     RTHIANLVVGWKMSREWQIGARWKYMTSETFTPIIADDKGRQIQNGRYVFNPTYSQNL
                     NSGRLKPYHRLDVRIDHFINYSWGFGNIFVEMLNVYLRDNPVNMSWNQARPISRTNPS
                     IQNDFGTLESSTGDGKKIKFPLINFGLEVQF"
     gene            8099..8644
                     /locus_tag="PLG78_01590"
     CDS             8099..8644
                     /locus_tag="PLG78_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21825.1"
                     /translation="MNAQTKKLPLVLAALSLCALFACSLDSRDEKKEKDLELQRWIYL
                     ALTTSRDVTGSCIAAETQGIACASAAGQTVTYGITISSVYGTSSSSANAATLCNDIPN
                     SALYTSPSANFSAAAKICHFGCYRSYFQNQITQNRCASQFTSIMTTYTNCLPLAWLVT
                     CTDTTLYNCLNSCFRTGTVLP"
     gene            complement(8723..9388)
                     /locus_tag="PLG78_01595"
     CDS             complement(8723..9388)
                     /locus_tag="PLG78_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE21826.1"
                     /translation="MIRFILSAALLFALTAPAFAQDSTCYIFNRIPVTGVDEIAKQLS
                     DPKVRLEDKSCAVSRLGELSRELVNNKNVAPSSIYNPILGSLAPQTQTADHHILREAA
                     CQALARFSDLPESERLIQPLGKVVKEDSHEEVRLACARALSRFYKDAAGATTELVAAL
                     NSELERGPQADNVTVATAIIRSLGSLRDRRSFVPLMRVIQSRFPTSTKREAQRALENI
                     KWD"
     gene            complement(9385..9885)
                     /locus_tag="PLG78_01600"
     CDS             complement(9385..9885)
                     /locus_tag="PLG78_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE21827.1"
                     /translation="MHVEEEQKNISKRWRYYIDKSFQNQFMARFALVIVLVTVATLGV
                     LAVLFENPYDLLPDAGVLHSMEIKRIPQPDGTFVETPVPGKTYNAFELFWKPILFISL
                     LNLIIIVVFSLFYSHSMAGPIHNIKVSLKDLAEGGKPKAIRIRKSDQFQDLAELLNEV
                     IEKRVK"
     gene            complement(9990..13487)
                     /locus_tag="PLG78_01605"
     CDS             complement(9990..13487)
                     /locus_tag="PLG78_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE21828.1"
                     /translation="MNLKSILGSAVVLTALSCATAPPPTISNAREDAGANTEAHRQSR
                     EWLNKANRQFQDGKFEESLASVRKSLQLEESFDGYFLEGNDLMKLGDQEEALRPYLKA
                     EKLNPKNEQLGLFLAMLYTSRGQLDQAQIRYVRLMDSYPEEPLYAFKAGTGYKLMKDY
                     NRAYSTLKEADKPAFKNRDQAYLQLGDVCVELKKYDEAEMYFKKAREVNPKLGDASIG
                     SAAGKTAKLLEEGNALFRQKRFADALTKFDEAKKESPTSTAPWYLAGSCRYALSQYPA
                     AQQDLQQMLKLQPENTDGLLLLSSALQKQGKHVEAEKIIRGEIKKSPGNAELYNRLGS
                     IHADQGLKKQAIEAYHRSLTLQPNYVPARINLAFAYLDDGRYSDAEREFQNALAQDPQ
                     NADLQKGKQLVGVYRALDLGDKNFRARKFDKAIADYNTALKIRTDLPVVTNSIGRAEF
                     EKRAFPAAEAQFKKSLALDPENTAAMVGLVKVYRAQGKKADADSYSAKVAAIAKSNPA
                     RAVELGRLKEEENPKEAEDYYSKLLKERPDDTAVRTRLASLYYKQGVDANKREQYDQA
                     RTLFKKSKDLAEVQGVDEALSIVEDNIAHAPLLPGLKRAEDLYNRGRFDEALPLYEQV
                     YSRWNKSLILVKIASCKMELGRKEEAIRALNDAAEKRPEDLELTEAVFTYYLTLGETD
                     RAKAGFEKILEQHEEAYYSLYKLGIIDLRAKSYKPAVEYFNRSLIYRPDFVEAKIARG
                     VARYQSGERDVARADFEEAMAQKSELAHLNVGMMQFNDNMLDQAQKIFEDLATKNPDY
                     PEPHYHLAYIHYSRGDLDKAEKEILQARKERDDPADLAAHAKILEKKYEQSRSPELAN
                     RIKEIHKTILRKYPASANAKVSRERLAKLEGSSVPVLVPYGGTAKILTPVLYQNLVIT
                     SENKSIQAYESGSRRVVWRRFFDKPVTSLAASQAIFSLEGETIHILDPDNGSDLGTLR
                     AEAGAKLLADSDRLLAVGPKGSASLYSLTDRIAVVAPEQVRFFLAGGVVYRLRGNNLE
                     RLNDKLAVEKSVAVKGGVITVAASGLLTLVLVPGELVIYDAALSKVKSIPVGRGVDHL
                     SVASTGFLAYSSTKVIQFDPDGDKKAERNGEAIQSAALLGDGRVVLVDGQEVRILDSA
                     GKQVWSSVLGQEKIHSLFY"
     gene            complement(13484..>14492)
                     /locus_tag="PLG78_01610"
     CDS             complement(13484..>14492)
                     /locus_tag="PLG78_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21829.1"
                     /translation="ISHTEGISILRSPSSLRVASDTLRTEEIHNMLTDSIMGTLDVQS
                     SSAETIVSLKGSSEISIGPAPVSKKVLEGSYTLIAARKGHAPQQLPVKIFAGEKRRVF
                     IAWPDDPASATTAFVSAPTGLRLAIDDDVKGPTPIYLLNESAHKVEFARKLADGRFEV
                     VGTANPSGDDRNRAMLLKYTEAFMEGYLESDIWQMVTEKSVKPLKGIRSRPFIVDEQK
                     TTVSFPLSEGSAFIAWVSDQDSIFVERIGNVFTVQGGKGSAMSGPKKAFQPIKEKPDR
                     TILIEYNSKKSIVTVELDGTSIFEGPFRPSATGQIVILGPDQMPIKKLDVRTGRGVYE
                     E"
BASE COUNT         3776 a         3715 c         3547 g         3454 t
ORIGIN      
        1 tgtcttctct ttgcatcctc gccatccttc tcttctacaa tagactcgaa gggattccgc
       61 gtacggtgct gatactcgat tctgtacttg ttttcctgct tcttggcttc cgtagcttta
      121 gctggagact gtttcgcgac atatactttg ttcgtcgcgc gcgcggcggc aggcgcaccc
      181 tgctcatcgg agccggagtg acggcaaatc gactggttac ggacctgaga accaacgagt
      241 cgctgcaaat catgccgatt ggctatctag acgatgaccc ggccaaaggc ggggcgcatc
      301 ttcagggact tccagttctc ggaaagattg aagacctcga tcacgttctc actgaaaaac
      361 ggatcgctga agtgatcatc agcgcaaaac ttcctggatc cgttatacgc catatctacc
      421 gtacgtgcga aacaagacac attccatgca aaactctgcc acctctctct gagcttcttg
      481 caagaccgga cattgggaag tccattcgca atgtaagcct ggaagatctg ctcggcagag
      541 acgtagtacg cctcgaaaca gaaggcattc gcaaggcaat cgaaaacaaa gttgttctca
      601 tcactggcgc cggtggttcg attggcagcg aagtctgccg acagatccta acgtattctc
      661 ccggtgtcat tgtgctgttt gaaaacgcgg aaactccgct ctacgaaatc gactacgagc
      721 ttcgcaagct ttgccttgaa aacaatcttc gcacacatat agacgcggtg attggagacg
      781 tgcgcaacgt agaagacctg gcacaggtat tcagacagaa cgaaatcaat actgtattcc
      841 attgcgcagc ctacaagcat gttcccatga tggaacttca tccagaagaa gctgtgaaga
      901 acaatgtact gggcacgatc aatgtcgcaa atgctgcccg cgatgctggc gtagaaagat
      961 tcgtcatgat ttctacggac aaggccgtaa accctgtcaa tgtaatgggt gcaaccaaga
     1021 ggattgctga aatctacatt cagaatcttg cgcgcgtaac agacacgcgt tacataacgg
     1081 ttcgattcgg aaacgtactt ggttcgaatg gttcagtgat tccgttgttt cagaaacaaa
     1141 tcgaagcggg cggcccagtt acagtcactc atccggacat cattcgctat ttcatgacca
     1201 tcaaagaagc ggcgggcctt gtcattcagg ctggcgtcat gggtcagggc ggagaaatct
     1261 ttcttctgga tatgggcgag ccggtgaaaa tcaaagacct ggcagaagac ttgattcgat
     1321 tcgcggggct tgtgccgcac caggacattc agattcatta cattggtttg cggccaggtg
     1381 aaaaactata cgaagagctt cttctcgccg aagaaggaat tgaaccaacg cggcacgaga
     1441 aaatccatat cgcaaactcc aggatttcgc accttcctac tctccttgaa aaagtacatg
     1501 aactcgaaga agcagccaga cgcggatcgc gtccggatat ggacgcgatc atgtcatcca
     1561 ttgttccgga atacagaccc gtaagtccga tgattcgccc ggcggacaga gcgcaggaaa
     1621 aagaaaacgc tcgatcgcga aagatctagc aatccactca tctcagactg gagtttacca
     1681 gacttcaata tcgtagacgg actttgcttc gcgaaatagg aggagcaaag aaaagaacaa
     1741 gaacgttccc gaaacgatgc gtcccttcca ctgtcgcgcc gccagtaaaa gtccagcaca
     1801 tacgggcaag aacaggcgcg ccacatttgc gaagttatcc cagtactctg aagatgcagt
     1861 cagagaaagt aaaaaaccac aagcaatcaa agccgcgcct gccaggaatc gctcctgctt
     1921 tagatccagg cgaaagagga caatgcatcc aaggatgtag aataaaaaca gcaaactgcg
     1981 gccggaaagg agtgcatccg gatgctgcac gtaattccat acaccgggcc cgcccgcagt
     2041 ccctctggta gcagcaaaga aaggggaaaa accagtcgca aaccaccaga caatcatagg
     2101 caaacaggca acgaccagag ccacaaacac gggtttgcga aatcgaagga atagaaataa
     2161 agcggataga gcgccggccg ccgcggtttc tttgcagagc atgctcagcg cggctatgat
     2221 caaaacgacg agcgaatgtt tttccttgaa tgcaagctcc agagcaagaa ccaatagcga
     2281 aagcgccatg ccgtctgcca gaaacaaccc cgctgacaaa acagagatcg gattgaacgc
     2341 gaagatactg gcgcgaagcg gatcggatct aagaaagaat acaacaaaca aatgaagtgc
     2401 aaggggaata aaatacatgg agtaaagaag gtagcctgga ccagccagcg cccccattcc
     2461 tgcgagcact ggataaccta tgcgagccag gcgaaaagga gcagaatcaa attcaacctg
     2521 aactccgcga aaaacgcgca tcgcagaata gaaataaaac tgcccgtcat agccgccgct
     2581 cttatagact acggcattct caggaagaat cgccttgttc ttcatcgcat aacagatagt
     2641 aggtgaaagg cagccaaatc gaacggcagc agacaaatgc cccgccttga aagcgggaat
     2701 ggcgagagct gccacatgaa gcgcggcgga cgccagaatc agaaatacca ggctcgatct
     2761 gctcatgcac tgatggcgcc gtcttccacc atcagcaggt tctgacagga cggacactga
     2821 tgtctgcctg ctttttcaaa acgaattgcc gcaccgcaga aaggacactg gccgatccca
     2881 cgcgaaaggc aatcaggctg aatgaccgca gagtcctccg ttacagaaac accgcacaga
     2941 ctgagttgaa gcgccagatc aggtggaaca ctgtagagga tcgtctggga cttttgcttt
     3001 tctacagact tgagaatttc ccgaacgcct tcgaaaaaca cgggcttcca ttgactgcaa
     3061 tccactgccg caacttccac agcaaacagt tctttgactg aggctggatc aagagggcat
     3121 gctggtttgc ggacattcgc catagatcat ttgcagaatg atttgcgctc ctggcaacca
     3181 tcttccactc tggattcatg gcaggagtga acaaagctcg cctgatctgg acatggaccc
     3241 aggaggcagt gtggctggcg ttgctagtgc ttcagctccc gtttgtcttt gtccagaaat
     3301 ggtcttcgca tggaagacgc aaagagcccg ttctcttgct cacggatctg ggtttgagtg
     3361 ttttcgctta ctttcgcatt cgaatggctc tggcccgggc cggcttcccg gtgattgttc
     3421 tttcgatcgg aagtgtttct tctctctcct ctcaggccag gcagctggcc agaacacttg
     3481 aacagctcaa cattcaaaaa ggaatcctgg tggctcacgg aaggggcggt ctgactgcgc
     3541 tggctcttcc ggattctgga agaagacgta tccagcacct cgtgactctg ggtactccgt
     3601 ttcacggttc gaggttcttc actcccttcg cttttgttcc ggccttcgga gacatgaatg
     3661 ttggaagtga cttcttgttg cttcatcgca tgaacgcgct cctgtttccc ggattctctc
     3721 ctttcagcgc ctacaaagat gaattcattt atccaggaaa cctcgggcac tttggtcagg
     3781 gacgagatct gatactcgat cgcaatggtc atatgaatct gattctcgac ggagagaaca
     3841 tagagacgat tgtacagcac atcgaatctc tccatccaga gcctgcggca aatccggaag
     3901 tccgtgccgc caaaccgccc gaagtcgcac ctccggcggc aaaaaagtcc gcgaaaaagt
     3961 cgtcaaagaa aaagccatcc aggccaggcc gtaagaaagg aaagcgttga atctaaagga
     4021 gattcaacga gtcatcgacg actggattgg gaaaaacggc gggtattatt ctgagctcac
     4081 caatcttgct cgtttgacgg aagaactggg agaactggct cgcctctatg ccaggcgcga
     4141 aattcccgcg cgagacctgg aggatgtttc gaggcacaaa atgcaggaag agatgggcga
     4201 catagtattt gccctgtgcg tacttgccaa tcaggccgga attgatcttg agacttctgt
     4261 gcaagatgtg ctgaataaag tcaagttgcg cgacgcagac cggcattaga agttgccact
     4321 cccctgtcgc ctttgaaatc tgccacatgc gcgcaatcct tgcttccatc gcatgtctac
     4381 tgctggttca gtgtgccggc gcaaacgcag gcatcgcggt gtccaatgtt cctacagaag
     4441 gccagactta cacggttctg ggcactgccg aaaccaccaa aagctggtgg agtctggatc
     4501 tggctgtgct ggcgtttccc ctgggtgaac ctccggttgc ggaagctgta gaagatttac
     4561 tcaagcagaa agagggaaat gccctcatca atcttcgatt ctggacagac agaaccacca
     4621 tcctgttcat gacccgtcat agatttcatc ttcaggcaga cgttattcgc tttgatccaa
     4681 aggttgatcc cgacaaggac aagaaggaca aggcaaagga cgacaaagac aaactaaaga
     4741 aatgaaacga tttcttttca ttgcaatttt agctgcagga gcatgcaccc cgggcggcct
     4801 cattgacaaa ggaggctaca gggatcaggg cggaattcaa ccccaggcca gtcgcacgca
     4861 cggtgttgct tacaaaatcc tttgctatga ttcgcaggaa gcgcgaagaa gagaaggtct
     4921 ccccgaaggt gcagaaaagc cgccgggacc tcctgttaga atcgaagacc cggccactta
     4981 caaaggcgga tgcgaagaag aaggagaaag ctctgtcttc tttctcttca atatagtgcc
     5041 ggtcactccc cctctcaatc ccgagtatgc gattggaact gcggtccaaa agctagaggg
     5101 agacacgatg attggaatga agacctggca ggagactcac tactattcca tcctgggtcg
     5161 cgtgtccgtc ttcaaagtga aaggtcttgt catcaagttt gataacaaag aaccaacaga
     5221 ttcaaaatct gattccaaag acaaagacaa gaagggagac aaagccacag acaagaaaga
     5281 cgacaaaacg gataagaaag acctcaaagc agatcagaag aaatgaaagt catcgcattt
     5341 gctgtactgc tgctcctctc agggtgcatt gttgaaaatg acactgtaga tactgcagac
     5401 gcgtactccg aaatttacgc ttccattcaa ttcaaagcaa gacaatgcgg caatcaaccg
     5461 gcctatcctc ttctggtgcc agaaaatcca cccagttacg gaaccagact ctgttctctt
     5521 tccatcattc gccaggattg tccttttaca aattacccgc ttttctgcgt tgaaatgttc
     5581 ggcatcgatc tgccagggat cggtccgtga tgaagtggcg cgcgcttctt gttttctttc
     5641 tgctttttcc agcgactgta tttgctatcg acgtaaagat caagatcgaa gacaaaaagg
     5701 cgggcaaacc tgtttcagat gcgcaggtaa tcgtcatgga aacaaaagaa aagtttttct
     5761 ctgacgaaac gggagaagca atcattcgca tccccgcaaa aggattctac accatccggg
     5821 tgatttcacc gtcaggcgaa attgtgcagc ctcgtgtgga agtcatcagc cccaatcaga
     5881 cagtgacgat cttccttgga attgaagaag aaaagccaat acctacaaat accccgcaga
     5941 aggtaactgc ggaagaagga atcagcgtta caggtaaaag agaaaggcag aaagtttcgc
     6001 gttaccaggt gcgcattgat gaagtgaaac gaattccggg ccagttcggg gaagcgctga
     6061 gaggtctcga aacactgcct ggcgtcaatg ctccgccttt tgggaacgga gacatagcag
     6121 ttagaggagc aaacacaaat tcgaatacat atctgctcga tgatttgccc attggatatg
     6181 cgtttcattt tgccggcctc aacagcgtca ttcacaacga tcttgtaaag tccatcgacc
     6241 ttttcacggg tgcctatccg gtcacatacg gaaatgcaac cggaggtgtg gtggccatcg
     6301 acacaatcga cgaagtagag cgattcggtg ggcatgcgac gttttccctc tggtccgcgc
     6361 aggcactgtt caagggaaaa atcggagacg ggtactggat tggatcaggc cgtatgtcgt
     6421 atctggacat cactctccgt ccttacattc cggaaggtac tcaattcatt ccgcgatact
     6481 gggacggaca attcaaggca atgcagagac tgagtcagac tcaaaaactg tacgtctacg
     6541 ctcttggagc gaaagacaca ttcattgcga agattgacaa caaaccgtcc tgggacccta
     6601 cggcagaatt tgatcccaga ttcgtaggag cgcaaatcgc attggatcaa gccttccaca
     6661 cagaagccat caaacacgtc tggaatccag gaacgcgcat caacaacgaa ctcactctct
     6721 actatcacaa taacattcag tacattgacg gacagcttgg tttgctcaga ccaaagcaga
     6781 aaagagagtc cggctggggt gctttcaaaa acgaattcga ctgggaaata tacaaagagc
     6841 acctgttcct ggacgtgggc ctggaagcac gcagatacga gtaccagaac aacggacagg
     6901 caacgtatct ggtaaaccct aacaatccca gtccggatcc atacaagagc gtaaaccctg
     6961 actttgtgaa tcttcccgtc cacgatagcc agatgacatc ctataacact ggttatacga
     7021 tgctcactct caaagcctgg ggcttcgaag caaagccagg cgttcgagta gactattttg
     7081 ggcttacaaa gcaaagagtg gccgatccgc gcggcactct gtcctataca gccccgtgga
     7141 aaatgaccat cagtgcgggc ggtggagtgt atcatcgttt gccggacgcg gaaacctatt
     7201 cacgctcttc gggcaatccg aatctaaaaa tggaacgcgc agagcactac ggcggaggaa
     7261 tcgaacagat cattgaatcc tggactctaa aagcggaagc gtatcgtcat tacttcacag
     7321 acatagtggt ggccgatcct tacatcacaa cacctgtgag actcaaccag gatccattca
     7381 atcgctatac gactccgtac atcttcaatg cacctctgaa cttctcaaat gacggcactg
     7441 gcttctcgga aggctgggaa ctttatataa agaaaagcaa accagacaac aagaatggcc
     7501 tgtacggatg gatctcctac acctggtcca ggactcttcg caacaatcat cagcacatca
     7561 tcacagatga tgaaaaacgt caaatctaca gcgcggatga agcacgtatc atccacgaat
     7621 acgataatac caaagatcac tacgctgatt ttgatagaac acacattgca aatctcgtgg
     7681 tcggttggaa gatgagccgc gaatggcaga ttggtgcgag atggaagtac atgacttctg
     7741 aaacattcac cccgatcatc gcagacgata agggcaggca aattcagaac ggaagatatg
     7801 tattcaatcc tacctatagc cagaacctga actcaggaag actcaagcct taccacaggc
     7861 tcgacgttcg cattgatcat ttcatcaact actcgtgggg ctttggcaat atcttcgtgg
     7921 aaatgctcaa cgtctatttg agagacaatc cagtgaatat gtcctggaac caggccagac
     7981 cgatatcaag aaccaacccc tcgatccaga atgactttgg aactctagaa tcctccacag
     8041 gggatggcaa gaagatcaaa ttcccgctga tcaactttgg ccttgaggtg caattctaat
     8101 gaatgcgcag actaaaaagc tccctctggt gcttgccgcg ctttctctgt gtgctctatt
     8161 cgcctgctct ctggattccc gcgacgaaaa gaaggaaaaa gacctggaac tccagcgctg
     8221 gatctacctg gctctcacaa cgtcgcgtga cgttacggga agctgcattg cagcagaaac
     8281 acagggaatc gcctgcgcct ctgcagccgg gcaaacggtg acttacggca ttactatcag
     8341 ctctgtgtac ggtacaagct cctcgagtgc aaacgcagcc actctatgca acgacattcc
     8401 gaattctgct ctctacacaa gtcccagtgc gaacttctct gcagcagcaa agatctgcca
     8461 tttcggctgc tatcgctcct attttcaaaa tcagatcaca caaaacagat gtgcgtctca
     8521 attcacatcc atcatgacca cttacaccaa ctgccttcct cttgcatggc tggtcacttg
     8581 tacagacact accctttaca actgtctgaa ttcatgcttc cgcaccggaa ctgtgctacc
     8641 gtaaggacat aaaaaaaccc ggccattgac cgggtttcgc atcttgagta gctagaatgc
     8701 tactggcaaa gcaattcgcg catcagtccc atttaatatt ttcaagagct cgctgagctt
     8761 cgcgcttggt agaagtaggg aacctggact gaatcactct catcaaagga acaaacgatc
     8821 tacgatcgcg aaggcttccc agagaacgaa tgatcgcagt ggctacagtg acgttgtctg
     8881 cctggggacc acgctccagt tcgctgttga gtgcagccac cagttcagta gtcgcacccg
     8941 ccgcatcttt gtagaaacga gacaaggcgc gcgcacatgc caggcgaact tcttcatgag
     9001 aatcttcttt gaccactttg cccagaggct ggatgagtct ttcgctttca ggcaaatctg
     9061 agaaacgagc cagcgcctgg caggctgctt ccctgagaat gtgatgatct gcagtttgcg
     9121 tctgcggtgc cagagatcct aaaatcggat tgtaaatgct ggaaggagcg acgttcttgt
     9181 tgttcacaag ttcgcgggag agctctccca gtctagaaac agcgcagctc ttgtcttcga
     9241 ggcgaacctt tggatctgac aactgctttg caatttcgtc cactcctgtt accggaatgc
     9301 ggttgaaaat gtagcaagtg gagtcctgcg caaatgcagg agctgtaagc gcaaataaaa
     9361 gcgccgctga aagaataaaa cgaatcattt gaccctcttc tctatgacct cgttcaacag
     9421 ttctgcaaga tcctggaact ggtccgactt acgaatgcga attgctttgg gctttccgcc
     9481 ctccgcgaga tctttcaggc tcaccttgat gttatggatg gggcctgcca tactgtgcga
     9541 atagaacaga ctgaagacta cgatgatgat cagattgagg agactgatga aaagaatcgg
     9601 tttccagaaa agctcaaatg cattgtacgt tttgccaggt accggagttt ccacgaaggt
     9661 tccgtctggc tgaggaatgc gtttgatttc catgctatga agtacgcccg catccgggag
     9721 tagatcgtat gggttttcga acaataccgc aagcacaccg agagtggcaa cggttaccag
     9781 gacaatcacg agcgcgaaac gcgccatgaa ttgattctgg aatgatttat cgatgtaata
     9841 gcgccagcgc ttgctgatgt tcttctgttc ttcttccacg tgcatctccc ccacgagatt
     9901 ccggtctttt ccagggtact gtcaatcaat tttcggcaac cagcctcccg gaatcgagag
     9961 gaggctgcgg cgaaaaccga atgggatcat caataaaaca gagagtgaat cttttcctga
    10021 ccgaggacag aactccacac ctgcttgccc gcactgtcca ggatgcgaac ttcctgaccg
    10081 tccacaagaa cgacacgtcc atcacccaga agtgcagcag attgaatggc ttctccattt
    10141 cgctccgcct tcttgtcacc atcgggatca aactgaatta ctttagtgga ggagtatgct
    10201 aaaaaccctg tgcttgcgac agaaaggtga tccaccccac gaccgactgg aatgcttttc
    10261 actttagata acgcagcatc gtaaatgacc agctctcctg gaaccagaac cagagtcagt
    10321 agtccggatg cagctacagt gatcacgcca cctttcactg cgacggattt ctctactgca
    10381 agtttatcgt tcaagcgctc cagattgttc ccgcgcagac gatagacgac tccgcccgcg
    10441 agaaagaatc gaacctgctc cggtgccacc accgcgatgc gatctgtgag agaatagaga
    10501 gacgcagaac ctttcgggcc aaccgcaagc aatctatctg aatctgccag gagctttgcg
    10561 ccagcttctg cacgcagagt tcccagatca cttccattgt ctggatcgag aatgtgaatc
    10621 gtctctccct ccagagagaa gatcgcctgc gatgcggcca gagaagtcac aggcttgtca
    10681 aagaaacggc gccatactac gcgacggctt ccggattcgt acgcctggat gctcttgttt
    10741 tcagacgtaa tgaccaggtt ctggtagaga acaggagtca gaatctttgc agttccaccg
    10801 tacggaacga gaacaggtac gctcgatcct tccagtttag ccaggcgttc gcgagatact
    10861 ttcgcattcg cagacgctgg atacttgcga aggatagttt tgtggatttc tttgattctg
    10921 tttgcgagtt caggacttct ggactgctca tatttctttt ctagaatctt tgcgtgcgca
    10981 gcgagatctg caggatcatc cctttctttg cgagcctgaa gaatttcttt ttcagctttg
    11041 tccaggtcac cgcgtgaata gtgaatgtac gccagatggt agtgcggttc cggataatcc
    11101 ggattctttg tggccagatc ttcaaatatc ttctgcgcct gatcgagcat attgtcattg
    11161 aactgcatca tgcccacatt gagatgagca agctcggatt tctgggccat ggcttcttcg
    11221 aaatcagcgc gcgcaacgtc gcgttcgcct gactgataac gcgcaacacc acgagcgatc
    11281 tttgcttcta caaaatcagg tctgtagatg agcgacctgt tgaagtattc cactgcgggc
    11341 ttgtacgatt ttgcgcgaag atcaatgatc ccgagtttgt aaagagagta gtatgcttct
    11401 tcatgctgct ccagaatctt ttcaaacccc gccttcgcgc gatctgtttc ccccagagtg
    11461 aggtagtatg tgaacactgc ctctgtcagc tcgagatctt ccgggcgttt ttcagccgca
    11521 tcattgagcg cacgaatggc ctcttccttg cgacccagtt ccatcttgca cgaagcaatc
    11581 ttcactagaa tcaaggactt attccacctg gaatacacct gctcgtagag aggaagcgct
    11641 tcatcgaacc ttccacgatt gtagagatct tctgctctct tgagtcctgg aagaagaggt
    11701 gcgtgtgcga tgttatcttc aacgattgaa agagcctcgt ccacgccctg cacttctgcc
    11761 agatcctttg acttcttaaa tagagtgcgc gcctgatcgt actgttctct cttgtttgcg
    11821 tctacacctt gcttatagta gagagaagca aggcgggtgc gcacagccgt atcatcgggg
    11881 cgttctttga gcaatttgct gtaatagtct tccgcttctt ttgggttttc ctcttccttg
    11941 agacgaccga gttccacggc ccgggcagga ttgctttttg cgatcgcagc caccttcgcg
    12001 ctgtaggagt ctgcatccgc tttctttccc tgtgcgcgat acacttttac aaggccaacc
    12061 atggcagctg tgttctctgg atcgagagcc aggctttttt tgaattgagc ttctgctgcg
    12121 gggaatgctc tcttttcaaa ttcagcgcga ccaatggagt ttgtgactac aggcaaatcg
    12181 gttctgattt tgagcgcggt attgtagtcc gcgattgctt tgtcgaactt gcgtgcgcga
    12241 aaattcttat ctccgagatc cagggcgcga tacactccaa ccagttgttt ccccttttgg
    12301 agatccgcat tctgtggatc ctgtgccaga gcattctgga attcgcgctc cgcgtccgaa
    12361 taacgtccgt cgtccaggta ggcaaacgca aggttgattc tggcaggaac atagtttggc
    12421 tgcaaagtca gtgagcgatg atacgcttca attgcctgct ttttcaggcc ctgatctgca
    12481 tgaatggagc ccagacgatt gtagagctct gcattgcccg gcgatttttt gatttcaccg
    12541 cgaatgatct tctctgcttc cacatgtttg ccctgctttt gcagcgcgct cgaaagaagt
    12601 agtagcccgt ctgtattttc aggctgaagc ttcaacattt gctgaagatc ctgttgtgcc
    12661 gcaggatact gagaaagcgc ataacggcaa gatccagcca gataccacgg agccgtggat
    12721 gtgggagatt ccttctttgc ttcatcgaac ttagtcaaag catccgcaaa tcgtttctga
    12781 cgaaagagag cgttgccttc ttccaataat tttgcagtct tgcccgcggc agatccgatc
    12841 gacgcatctc cgagcttcgg gtttacttcg cgtgcctttt tgaaatacat ttcggcttca
    12901 tcgtatttct tgagttcaac gcaaacgtct ccgagctgca gatatgcctg gtcacgattt
    12961 ttgaatgcag gtttgtccgc ttcttttaaa gtgctgtaag ctctgttgta atctttcatg
    13021 agcttatagc cagtgccggc tttgaatgcg tatagaggct cttccggata ggagtccatc
    13081 aatcgaacat agcgaatttg cgcctggtcg agctgaccac gcgaagtgta gagcatggcc
    13141 agaaacagtc ccagttgttc gttcttcgga ttcagtttct ctgctttgag gtaaggtctg
    13201 agagcttctt cctgatctcc cagcttcatg agatcatttc cttccaggaa ataaccatca
    13261 aaactttctt cgagttgaag actctttctc acgctggcga gggactcttc aaactttccg
    13321 tcctggaact gacgatttgc tttgttcaac cattctctgg actggcgatg cgcttccgta
    13381 ttggcgccag cgtcttcgcg agcattagaa atggtgggtg gaggtgccgt ggcacaggaa
    13441 agtgcagtca gtacaactgc agagccaagg atggatttaa gattcattct tcgtataccc
    13501 ctcgtccggt gcggacatcg agcttcttga tcggcatctg gtctggtccc aggataacga
    13561 tctggcccgt agcagaagga cggaatggtc cttcgaaaat ggaagttcca tccagctcaa
    13621 ctgttacgat ggacttctta ctattgtatt ctataaggat ggttctatcc ggcttttctt
    13681 tgattggctg gaatgctttt ttgggaccag acatggcaga gccttttccg ccctgcactg
    13741 tgaatacatt tccgattctt tctacgaaga tagaatcctg atccgaaacc cacgcaatga
    13801 acgctgaccc ttcactgagt gggaaagaaa cagtggtctt ctgctcatcc acgatgaatg
    13861 ggcgagagcg aattcccttc aaaggcttca ctgatttctc agtcaccatt tgccagatat
    13921 cgctttccag atatccttcc atgaacgctt ccgtgtattt caaaagcatg gcacggtttc
    13981 tgtcgtcgcc tgacggattg gcagtgccca ctacttcaaa tcttccgtcc gcaagcttgc
    14041 gcgcgaattc cactttgtgc gcggattcat tgagcaaata gatgggagtc gggcctttga
    14101 catcatcgtc tatggccagg cgaaggccgg tcggtgcact tacaaaagca gtggtcgcag
    14161 aagcaggatc atccggccat gcaatgaaca ctcgcctctt ctcgcctgcg aatattttca
    14221 ctggaagttg ctgaggagca tgcccctttc gcgcggcgat cagagtgtaa gaaccttcca
    14281 gaactttttt tgaaacaggg gccggaccaa tgctgatctc agaggatcct ttcaaggata
    14341 caatggtctc agcggatgac gactgcacgt cgagagtgcc catgatgcta tcggtgagca
    14401 tgttatggat ttcttctgtg cgaagagtat cgcttgcaac gcgcagactg gacggagaac
    14461 gaaggatgga gattccttct gtatgtgaaa tt
//
LOCUS       DAOQJL010000020        14465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2014_length_14465_cov_4.288827, whole genome shotgun sequence.
ACCESSION   DAOQJL010000020 DAOQJL010000000
VERSION     DAOQJL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2014_length_14465_cov_4.288827"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLG78_01615"
     CDS             complement(<1..375)
                     /locus_tag="PLG78_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNE21830.1"
                     /translation="MPISEIPGKIPVKLWTRLEDVESGALVQLKNIASLPWVFRHVAA
                     MPDVHLGIGATIGSVIATREAISPAAVGVDIGCGMGAVQTSLKASDLPDHLGAIRSRI
                     EAMIPVGHKEHSQARVRKSEVKF"
     gene            complement(375..2450)
                     /gene="uvrB"
                     /locus_tag="PLG78_01620"
     CDS             complement(375..2450)
                     /gene="uvrB"
                     /locus_tag="PLG78_01620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNE21831.1"
                     /translation="MAEERRFRLVSEYKAAGDQPHAIAQLVDAYNQGKQKLCLVGVTG
                     SGKTYTMASFLEGIQKPALILTHNKTLAAQLYREFREFFPENAVEYFVSYYDYYQPEA
                     YVASSDTYIEKDASINEEIDRLRLRATASLLERRDVVIVSSVSCIYGLGSPEEYSHSL
                     VMIDQGLEIDRNDVIRQLLHIQYVRNDIAPARGTFRARGDTLEIFPAYRQEGIRVEWF
                     GDEIEAISMFDPLTGRSLDKMKRAIIYPAKHFITSPPRLKDAVEVIRQELEAQIAFFK
                     EKGKLLEAERIDHRTRYDLEMLTEMGYCNGIENYSRPLSGRKAGERPACLIDYFPDDF
                     VVIIDESHVTIPQIGGMFEGDRARKQTLVDFGFRLPSALDNRPLNFKEFESLAKNVMY
                     VSATPADYESKNAEVTVEQLIRPTGLLDPEVEVRPVKNQIEDLMLEVRKRTAVSERVL
                     ITTLTKKMAEDLSDYLSDLDIKVAYLHSEVQTMERVEIIRDLRKGKYDVLVGVNLLRE
                     GLDIPEVSLVAILDADKEGFLRNARSLIQTIGRAARNLHGKAILYADKITPSMENAMA
                     ETKRRRAIQAAHNEKEGITPQTIRKEIYDMIERENLADVEEKIVEEIGAQLKITDEMS
                     AKEQMEELRRAMLQAAANMEFEKAALLRDAMLSVEKGEKVDFMKLAQKKSGKKQGQTT
                     QMRGRKGRR"
     gene            2590..3048
                     /locus_tag="PLG78_01625"
     CDS             2590..3048
                     /locus_tag="PLG78_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNE21832.1"
                     /translation="MNNKYVFGAAGVVLGACLTVGAGAAMWRCYGPMGSPETRAAWVV
                     KKLSSDLDLDATQIQKLTAIKDEVLKHRAATASGREEMHKAVLAQVESANVDKAALNK
                     IFDSHMSDMKDSRVFLIDKFAEFHSILRDDQKKTLKKKMEHFLMSHDAPI"
     gene            3057..3566
                     /locus_tag="PLG78_01630"
     CDS             3057..3566
                     /locus_tag="PLG78_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE21833.1"
                     /translation="MTEAEFTEIVESTKRVVLSAIEKNLYPRFLHAIDDVVQETYLRA
                     YKSLQKKQFEGRSALTTWLYTIARNESIRMNEKLGREDKKIAKLQAEPDREPAPQSDN
                     NSEDMMGWVKRLPARYMQVIKMYLGGSSEKEIADRLEISPGTVKSRAHRAREMMRKMM
                     EKEGQHAVK"
     gene            3553..3855
                     /locus_tag="PLG78_01635"
     CDS             3553..3855
                     /locus_tag="PLG78_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21834.1"
                     /translation="MQSSDQALKHEIESRCSSHEWTCNVARMVSGTVRRRIRIRNTVI
                     AVTFSVLLPAGLTASGHMERAAYALLDSMVGVRHQLVIQPVTTEEVDTLIEIALNN"
     gene            complement(3845..5338)
                     /locus_tag="PLG78_01640"
     CDS             complement(3845..5338)
                     /locus_tag="PLG78_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE21835.1"
                     /translation="MKRSKIDNIYWTSFQEVSSYRPMIPLGPYLARHPRKQDALYHGL
                     RDVILGGSVEPDSRLPSTRTMARQLGLSRGTVVLAYEMLESDGLIRLDAGRAPVVLHR
                     AQERITVKAAPYKASLWASRLPVEEKSRKENETRTSKRIVDFNPGYTDPELFPAREWN
                     QALHRAIRTFDPQVAEAAGGLVTLRRGVASYLSKRRGMVVHADQIVIVNGSIQAIVIL
                     SHILLGENSKAVLENPGYRGIRDAVRLCGASPIFAAVDKEGIRVRDWDAQLCFVTPAR
                     QFPTGAALSYDRRLELVEWAERKNAFILEDDYDSEFRRRGRPLEPLQCLAPARVVHVG
                     TFSRTLAAGLRLGYAVLPENLLDPFLRAKRTFETHPAGTIEQSALSELMRSGAYERHL
                     RRAARVYSRRHDLLNRLLRDHFGDVLEIAESETGLHVFAKWKKGIALFNRFEQSCLDH
                     GIVPGSARRNYFTRYSPSLIFSVAHLSESQLTRALGQMAKILSAINY"
     gene            5382..5990
                     /locus_tag="PLG78_01645"
     CDS             5382..5990
                     /locus_tag="PLG78_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE21836.1"
                     /translation="MRRKEFVVEDATEIEQFLGEMSFGFLTQNRGEVPAITPLNYVVY
                     RGDICFHGSRVGEKMQLLKAGTAAAFCVAREVSIIPSFFSGAEIACPATAFFKSVVIR
                     GSLEEVTDLQEKCDILTALMEKLQPEGRYAPFDLEDPEYLKNVKGVSVVRLKTQERTA
                     KFKFGQNQTRTKWQQILDNLLRRGRPSDLEAASEMKKRCPFH"
     gene            complement(6019..6657)
                     /locus_tag="PLG78_01650"
     CDS             complement(6019..6657)
                     /locus_tag="PLG78_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21837.1"
                     /translation="MSQIALPPPVAPSIGINRFFVVSFVIHTLIAGGLYLYLYAWPAE
                     EHYVATVALYMGDAENDRETTGKGKGNKDASKDDKKTSSDTGTGPSTSSNADWGTASD
                     PSTEGGSRYAPDLWIDGNLEDFYPARAKQANLGKVTVRVSLYIDSTGKIREVRVQGVR
                     SAGNAHAPYEADFRQAAQQIALTRMRLRSAGYRKDGRPVDFIWDTVINFTLQ"
     gene            complement(6742..7767)
                     /locus_tag="PLG78_01655"
     CDS             complement(6742..7767)
                     /locus_tag="PLG78_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE21838.1"
                     /translation="MSLRYFGPRFFVVGYGSPSHAEVYSVNGDGQVTFVSQATGGNFP
                     VMLSADPTLNYIRMGSNSGGGYLTSFSLNRSTGSLAQVHEIPATGASLATADHPSLPF
                     VMNVVTGQVNVHPVLSGGVLGTAVGYDAGRTFSGARLYGNILYAADNSAAGTVRSYRL
                     GSTGVLTTLQTMPSSATNQATVGIEPLGRFMYVSNGSATNQVALFSLDGNGSMSGPTF
                     MTLSNGAGGTNFAFDPYGRYLFASRSIATNNLQTYAINQATGQLTLASMITSGTGPSQ
                     IVVDPAGRFLVHLDAAGVTMRVYSYSNGMLTPVTGSPFSIGPGTNPVALIAICYEKYA
                     FGELQFN"
     gene            complement(7858..8640)
                     /locus_tag="PLG78_01660"
     CDS             complement(7858..8640)
                     /locus_tag="PLG78_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNE21839.1"
                     /translation="MNLTLHVWRQKDKDDPGRMVEYKVGEAEGVNEHMSFLEMIDVLN
                     ERLISQKEEPIAFDHDCREGICGACSMMINGVPHGPEKGTTTCQLHMRKFKSGDTIYI
                     EPWRAKAFPVIKDLVVDRSGFDKIIQAGGFVSINTGNAVDANCLPIAKTDSDLAMDAA
                     QCIGCGACVATCKNASAMLFVAAKVSHLGLLPQGQPERDRRVLEMVAVMDEAGFGNCT
                     NQFECEAACPKEISVDFIARLNRDYARAVARERPARQEMGAG"
     gene            complement(8651..10558)
                     /locus_tag="PLG78_01665"
     CDS             complement(8651..10558)
                     /locus_tag="PLG78_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNE21840.1"
                     /translation="MSLNSNIPSGPLAEKWDKHKFDLKLVNPANKRKFDVIVVGTGLA
                     GASAAATMAELGYNVKVFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSTMRLFYDTIK
                     GGDFRAREANVYRLAQVSANIIDQCVAQGVPFAREYGGTLANRSFGGAQLSRTFYARG
                     QTGQQLLLGAYQALSRQIGKGAVKMYPRTEMLDVVVHEGRAKGIVVRDLVTGKISSHS
                     GAAVVIASGGYGNVFFLSTNAMGCNVTATYRAYKKGAFFANPCYTQIHPTCIPVSGDY
                     QSKLTLMSESLRNDGRVWAPKNKGDKRHPSEIPENERDYYLERVYPSFGNLAPRDVAS
                     RQAKYVCDEGRGVGDTGLGVYLDFKDAIARLGESTIRERYGNLFQMYERITGEDPYKR
                     PMRIYPAVHYTMGGLWVDYNLMSNLPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     ILPYTIANYLAQVGPAKLPEDHAEFKKSEAEVTEMTNKFLSINGTRSVDSFHKELGKI
                     LWDHCGMGRNEAGLKKAIARIPELREEFWKTVRVPGSGSSLNPEIEKAGRVADFLEFG
                     ELMCRDALHRNESCGGHFREEYQEDGEAKRNDDKFCYAAAWEYNGKSEPKLNKEPLVF
                     ENIHLATRSYK"
     gene            complement(10558..11250)
                     /locus_tag="PLG78_01670"
     CDS             complement(10558..11250)
                     /locus_tag="PLG78_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNE21841.1"
                     /translation="MSFTQRIFSSSLGRKYLMAISGALLMVFLIAHMTGNLLVFAGPD
                     AFNAYGHYLKTHPQLLWPARIGLIVLFVLHVYTGISLARENRAARPTAYAFKNTIQAT
                     LASRTMALTGLLVLAYLIYHLLHFTIGIVQPQYFSGVDGQGRHDIYTMFILGFRNPII
                     YSSYVLAQIVLVLHLSHASRSFFQSMGWNQARFSGAIKTFSVAYTLVIFLGFLITPTC
                     VILNFIKLPGEL"
     gene            complement(11367..12029)
                     /locus_tag="PLG78_01675"
     CDS             complement(11367..12029)
                     /locus_tag="PLG78_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE21842.1"
                     /translation="MRKLQIVLVLIVSSSCTGSFPRTSLDSESQLLLARALFQSSSAT
                     SAAAVPSCASNAFCYTFRTNGLASANLGGISGADATCNSDVNKPAGATGSFKAFLVDP
                     QTNLRRASVTANLGDGQIDWVLRPSKQYRRSDGITILGTTGSNSLFTFALTNSFVSSA
                     GGHWTGLASTWVYDASHCTGWTSTAGSGRDGADNSTSTTAIFNNATLCNTSGPTIICI
                     EQ"
     gene            complement(12095..13267)
                     /locus_tag="PLG78_01680"
     CDS             complement(12095..13267)
                     /locus_tag="PLG78_01680"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE21843.1"
                     /translation="MSKLLEMRPGILTFLALAFGLSCQPVSQGNHSLETLLLADGSRT
                     TVWEPRILYLVGYSSPDLWAFQVDSTTGALTQIFTNTSTLAAVNALNVVVDSTNGVLY
                     VPRNINPGGLRSFSVSEAVPVPGTVSTNTAGVAFSHSAVNHPTLPMTYVVDTGGNVLV
                     YSRTAAGVFGAPSTFAIGTPTGSYLTISPGARFLFYRANSIIRTAPISSAGTLSTAVG
                     SYTSTGNGYLVSHPDGRFVYVTGTNSIDLLQLGDDGSLTLISQTATPNLNIPFVIHPN
                     GKYLYMTDSTGTAYVYWINKVTGALLLLQQISVNNSAPQASCIDVRGRFYYTFGSGAG
                     NIATAFRINDTNGTLSQTAAYTLSTSHRGCAFLNAQVSRSHVELRADEYLPSWLIL"
     gene            13433..>14465
                     /locus_tag="PLG78_01685"
     CDS             13433..>14465
                     /locus_tag="PLG78_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21844.1"
                     /translation="MKINQISPIATFLLGALFFTTNGCARHLSHGIEDWPFLIAYYDI
                     SYQDLCAPWVTEFSVIQPGMADRVQSYLQYQASLGSASSVGSTRLFGTVYSGNAKWEG
                     GALAPNGKIYGARFGAGPTMPFLEIDPNTLTTALVGPASNTGSGMVLLPSGLLLEIPH
                     TGVMNPVVLNPNTGATTTLSGSNGGVACSGGVLAPNGKVYCTAQSGTSGALEIDPVSG
                     SVTIFGAAQLGLNYGGSAVLGLNGKIYAVPYAGTTQIAEIDPTTRGITLFGPSLTANN
                     YYGGILAPSGKIYLPGFTTSTILEVDPIAHTVTSFGNTGGSSNYLGAAVAPNGHIYLT
                     PRSSAVVLEF"
BASE COUNT         3609 a         3770 c         3623 g         3463 t
ORIGIN      
        1 aaacttaact tctgattttc gcactcgagc ctgagaatgc tccttgtgtc ctaccgggat
       61 cattgcttct atgcgcgatc ggatggctcc caggtggtcc ggaagatctg acgctttcag
      121 gctggtctgc actgctccca ttccacaacc gatgtccact ccgactgccg cgggcgaaat
      181 agcttcgcgt gtcgcaatca cagatccgat cgttgcgccg attcccagat gaacatctgg
      241 catcgcggcc acatggcgaa atacccacgg aagagaggcg atattcttca gctgcacaag
      301 cgcgccgctt tccacatcct caaggcgcgt ccacagtttg acaggaatct tacccgggat
      361 ttccgaaatc ggcactaacg cctgcccttt ctgccgcgca tctgtgttgt ctgtccttgc
      421 ttctttccgc tctttttctg agcgagtttc ataaaatcga ctttctcccc tttttccacg
      481 gacagcatcg catcgcgaag aagggctgcc ttttcaaact ccatattggc cgctgcctgt
      541 agcatggccc ggcggagttc ttccatctgc tcttttgcag acatttcatc agtgattttt
      601 agctgcgccc caatctcctc tacgatcttc tcctctacat ccgcgagatt ctcgcgttcg
      661 atcatgtcgt agatttcttt gcgaatggtc tgcggagtga ttccttcctt ctcattgtga
      721 gccgcctgaa tggcgcgacg acgttttgtt tctgccatcg cgttttccat agaaggagtg
      781 attttatccg cgtagagaat tgcttttccg tgcaagttcc gcgcagcccg accaatggtc
      841 tggatcaatg aacgagcatt cctgagaaaa ccctctttgt ccgcatccag gatggccacc
      901 agagagactt ctggaatatc cagaccctct cgtaacaaat tcacacctac cagaacatcg
      961 tactttcctt tgcgcaaatc gcgaatgatt tccacgcgtt ccatggtctg cacctctgaa
     1021 tgcagatagg cgaccttgat atccagatcc gaaaggtagt cggacagatc ttccgccatc
     1081 ttcttggtaa gtgttgtgat gaggacgcgc tcggacacgg cggtgcgttt tcgaacttcc
     1141 agcataagat cttcaatctg attcttgact ggccgaacct ctacttccgg atcaagcagt
     1201 cccgttggac gaatgagctg ctctaccgtt acttccgcgt tttttgactc gtagtctgcg
     1261 ggagtggcgc tcacatacat tacgtttttc gcgagacttt caaattcttt gaaattcaga
     1321 gggcgattgt ccagagcgga cggaagcctg aatccaaagt caactaacgt ctgtttcctg
     1381 gcacgatctc cttcaaacat tccaccgatt tgaggaatcg tcacgtgact ctcgtcaatg
     1441 atgaccacaa agtcatctgg aaagtagtcg atcagacagg cgggcctttc tccggctttc
     1501 ctcccggaaa gaggtctgga atagttttct atgccattgc agtatcccat ctccgtcagc
     1561 atttccagat catatcgggt tcggtgatct attcgctccg cttcaagaag tttccctttt
     1621 tctttgaaga aagcaatctg agcctccagc tcctgtcgga tgacttccac tgcatctttt
     1681 aaacgaggtg gagaggtgat gaaatgtttc gcagggtaga tgatggctct cttcatctta
     1741 tcgagcgaac gccctgtcag aggatcaaac atagaaatcg cttcaatctc atctccaaac
     1801 cattccaccc ggattccttc ctgtctgtat gctggaaaga tttcgagagt gtcccctctt
     1861 gcgcgaaacg tcccgcgcgc cggtgcaatg tcattgcgaa cgtactgaat gtgaaggagt
     1921 tgtcgaatca cgtcattacg atctatctcc agaccctgat caatcatcac aaggctgtga
     1981 ctgtattctt cgggagaacc cagaccataa atgcaagaaa cggaggacac aatgacaacg
     2041 tcgcgtctct caagcaagct tgccgtcgcg cgaaggcgca accgatcgat ctcctcgttt
     2101 atggacgcat ctttctcaat gtatgtatcg ctcgatgcta cgtacgcttc tggttgataa
     2161 taatcgtagt aagaaacaaa atactccact gcgttttccg gaaagaattc ccggaattct
     2221 ctatagagct gtgcagcgag agtcttgttg tgagtgagta tgagcgctgg cttttgaatt
     2281 ccctccagaa aggacgccat ggtatacgtt ttacctgaac cggtaacgcc caccaggcag
     2341 agtttttgtt tcccttgatt gtacgcgtcc accagctgtg ctatggcgtg cggttgatcc
     2401 cccgctgctt tgtattccga aaccagtcta aatcttcttt cttcagccac gggagaagga
     2461 agccaggagg cagacaacgg tcaactgcaa ggggccattt ttttgctatc tggaggcgcg
     2521 ggactggtta ttttgagatt cgcgggaacg aatggtcctc ccgccgggtc ttacgattgg
     2581 gaaacggcaa tgaataacaa gtatgtattt ggcgcagcgg gcgtggttct gggtgcctgc
     2641 ctgacggtgg gtgccggcgc ggctatgtgg agatgctacg gccccatggg atcgcctgaa
     2701 actagagcgg cctgggttgt aaaaaaactt tcatctgatc tggatctgga cgccactcag
     2761 atccagaaac tcacagccat caaggacgaa gttctcaaac acagagcagc caccgccagt
     2821 ggtcgcgaag agatgcacaa ggctgtgctc gcccaggtgg agtcggcgaa cgtggataag
     2881 gcagcactca acaaaatttt cgactctcac atgagcgaca tgaaagacag cagagtgttt
     2941 ctcattgata aattcgcaga gtttcattct attttgcgag atgatcagaa gaagacactc
     3001 aaaaagaaga tggaacactt cctgatgtct cacgatgcgc caatctgatg gggacaatga
     3061 cggaagcaga atttactgaa atcgtcgaaa gcacgaagag agtcgtactc tctgcgatcg
     3121 agaaaaatct gtacccgcgg ttccttcatg caatcgatga tgtagtgcag gagacgtatc
     3181 ttcgtgcata caaaagcctg cagaagaaac aattcgaagg cagatctgca ctgaccacct
     3241 ggctttatac gatcgcacga aacgagtcga ttcgaatgaa tgagaaactt ggtcgcgaag
     3301 acaagaaaat agccaaattg caggccgaac cagacagaga gcccgctcct cagtccgata
     3361 acaattcaga agacatgatg ggatgggtga agcggttgcc tgcgcgatac atgcaagtta
     3421 ttaaaatgta tttgggcgga tccagtgaaa aggaaatcgc agatcgtctt gaaatcagcc
     3481 cgggaaccgt gaagtccagg gcacaccggg caagagaaat gatgcgaaag atgatggaga
     3541 aggagggaca acatgcagtc aagtgatcaa gcattgaaac acgaaattga gagcagatgc
     3601 tccagtcatg agtggacgtg caacgttgcc agaatggtat caggcacggt gcgaagaaga
     3661 attagaattc gcaatacggt cattgccgtg acgttctctg ttctgcttcc tgcaggttta
     3721 accgcaagcg gccatatgga aagagcagcg tatgctcttc tcgattctat ggttggagtt
     3781 cgtcaccagc tggtaattca accggtaacc acagaggaag tagataccct catcgaaatc
     3841 gctctcaata attgatcgcg ctcagaattt tggccatctg gcccagggcg cgagtcagtt
     3901 gcgattctga caaatgcgcc actgagaaaa tgagagatgg cgaataacgt gtaaaatagt
     3961 ttcttcgcgc ggatccggga actatcccat gatccaggca gctctgctca aaccgattga
     4021 atagcgcgat tcctttcttc cattttgcaa aaacgtgcaa gcctgtttcc gattcagcga
     4081 tctcaagtac gtccccaaaa tgatccctca acaaacgatt caacaaatca tgtcgtctgg
     4141 aatagactct ggccgctcgc ctgagatgcc gctcataggc ccctgatctc atcaattcag
     4201 aaagcgcgct ttgttcaatg gtcccggcgg gatgtgtttc aaatgtgcgc ttcgcgcgca
     4261 agaaaggatc gagtagattt tcaggcagaa ccgcataacc cagcctgagt cccgcagcaa
     4321 gtgtacgcga gaatgttcca acgtgaacga cgcgagccgg agccaggcac tgcaatggct
     4381 cgagtggtct tcctcttctt ctgaattcgc tatcgtagtc atcttcaaga ataaatgcat
     4441 tctttctctc tgcccattcg accaattcca gcctgcgatc atacgaaaga gcggcacctg
     4501 ttgggaattg tctggccggg gttacaaagc agagttgcgc atcccaatcc cgaactcgaa
     4561 ttccttcttt gtctacggcc gcaaaaatgg gagaagcacc acaaagcctg accgcatcac
     4621 ggatgcctct atatccagga ttttcaagaa cagctttcga attctcaccg agcagaatgt
     4681 gagacaaaat gacaatcgcc tgaatagatc cattcacaat gacgatctga tccgcgtgca
     4741 ccaccattcc ccttctcttg ctcaagtagg aggccacgcc tcggcgcaat gtgacgagcc
     4801 ctcccgcggc ttctgcgacc tgcgggtcaa acgttcgaat ggcgcggtga agagcctgat
     4861 tccactctcg cgcaggaaac aactcgggat cagtgtaccc cggattaaaa tcaacgattc
     4921 ttttcgaagt gcgcgtttcg ttttcctttc tggatttttc ctctactggc aatcgagacg
     4981 cccacaggct ggccttgtaa ggggctgcct ttaccgttat gcgctcttgc gctcgatgca
     5041 gaaccacagg ggcccggccc gcgtccaaac gaatcaggcc atccgattcc agcatttcat
     5101 aggccagaac tactgtgcct cttgatagtc ccagctgtcg tgccatggtg cgcgtggaag
     5161 ggagccgact gtctggctca acactgcctc ccaggatcac gtcccttaat ccatggtaga
     5221 gtgcgtcctg tttgcgcggg tggcgcgcca gatagggacc caggggaatc attggcctat
     5281 agctggagac ctcctggaaa ctggtccagt aaatattgtc tattttggac cttttcattg
     5341 gtccagtttt atgatattct tctggttctg gagcaatcag catgagaaga aaagaattcg
     5401 tggtagaaga cgccacagag atcgaacaat ttctcggaga gatgtccttc gggtttctaa
     5461 cgcagaatcg tggcgaagtg cctgccatca ctcctttgaa ctacgtagtg taccggggag
     5521 acatttgctt tcacggcagc agagttggcg aaaagatgca gttgctcaaa gcgggtacgg
     5581 cggccgcttt ctgcgtcgcg cgcgaagtca gcatcattcc ttcatttttt agtggcgcgg
     5641 agattgcgtg tcccgcaacc gcattcttca aatctgtggt gatccggggt agcctggagg
     5701 aagtaacgga tctgcaggag aagtgcgata tactgacggc actcatggaa aaacttcaac
     5761 ccgagggacg ttatgcgcca ttcgatcttg aagatccaga atacttgaaa aacgtgaaag
     5821 gtgtgtccgt tgtgcgtttg aaaacccagg aaaggactgc aaaattcaaa ttcgggcaaa
     5881 accagaccag aaccaaatgg cagcagatac tggacaattt gctgagaaga ggaagaccct
     5941 cggatctaga agccgcgtct gaaatgaaga aaaggtgccc atttcactga cgggatctac
     6001 atccgtgtcc ggatagaatc actgcagagt aaagttgatg acagtgtccc agataaaatc
     6061 cacaggacgg ccatccttgc gataaccagc actgcgcagg cgcattcttg taagcgcgat
     6121 ttgctgcgct gcttgcctga agtcagcttc atacggagcg tgagcatttc cagcagaacg
     6181 cactccctgt acgcgcactt cccgaatctt gccggtggaa tctatgtaga gagaaacgcg
     6241 aaccgttacc ttgccaaggt ttgcctgctt cgcacgcgca ggataaaaat cttccaggtt
     6301 tccgtctatc cagaggtcag gcgcataacg actgccgcct tccgtagatg gatctgatgc
     6361 cgttccccag tctgcgttcg aactcgtgga aggcccggtg ccagtatcgc tggatgtctt
     6421 cttgtcgtcc ttcgacgcat ccttattccc ttttcccttt cctgtagttt ctctgtcgtt
     6481 ttcagcgtca cccatgtaga gggcgacagt ggccacgtaa tgttcttccg cgggccacgc
     6541 atacaaatac agatacaacc cgcccgcaat gagggtatga atcacaaaag aaacaacgaa
     6601 gaaacggttg atgccgatgg atggcgcaac gggcggtggc agagcaatct gagacatacc
     6661 tattcctttc tccgcgatgc cactttacgg taaataaaaa agatcaggat ggtttgacga
     6721 tgcgttttga gccgggcgcg atcaattgaa ctgaagctct ccgaaagcat acttctcata
     6781 acagatcgcg atcagggcca ctggatttgt gcctggtcca atagaaaacg gggaacccgt
     6841 gaccggggtg agcatcccgt tcgagtagga atacacacgc atggtaacac ccgccgcatc
     6901 gagatgaacc agaaaccggc ccgcgggatc cacaactatc tgcgaagggc cagtgcccga
     6961 tgtgatcata gaagcaagag tcagctggcc cgtggcctgg ttgatcgcat aggtctgcaa
     7021 attgttcgtt gcaatgctgc gtgatgcaaa cagatatctg ccatagggat cgaaagcgaa
     7081 attcgttcca ccagctccgt tgctgagtgt catgaacgtt ggtccgctca tcgagccatt
     7141 tccgtccaga ctaaacaatg cgacctgatt tgtggcgctg ccgttagaaa catacatgaa
     7201 acgaccaagt ggttcaatgc ccaccgttgc ctgattggta gcggaactgg gcattgtctg
     7261 aagagtagtc agaacacctg tacttccaag gcgatagctt cttacagtcc ccgcagcaga
     7321 gttgtcagcc gcatacaaaa tatttccata caacctggcg cctgaaaaag ttcggcctgc
     7381 atcgtaaccg acagcagttc ccagcacgcc accggaaagc acaggatgga cgttcacttg
     7441 accagtcacc acattcatga cgaaaggcaa tgatggatga tcggcggtgg ccagagaggc
     7501 acctgttgct ggaatctcat gcacctgagc gagactgcct gtggaacgat tgagtgaaaa
     7561 gcttgtgagg taaccgccgc cactgtttga acccatgcga atgtagttca gtgttggatc
     7621 cgcagaaagc ataacgggaa aatttccgcc ggtggcctgc gaaacaaaag tcacttgccc
     7681 atcaccattt acggaataca cttccgcgtg cgaaggcgat ccatatccta cgacgaaaaa
     7741 acgaggcccg aagtaccgaa gactcaagag ggaaagcagg agactccggt catcaggaag
     7801 ctcctgccgg caagcaacca gcagaaacat tcctgaaatc agagcccgca ttcgcatcta
     7861 gccggctccc atttcctggc gagcgggacg ttcgcgtgca accgcacgcg catagtcacg
     7921 attgagccgg gcaataaaat ccacactgat ttccttggga catgctgcct cgcattcaaa
     7981 ctggttcgtg caatttccga agccagcctc gtccatcact gcgaccatct cgagaacgcg
     8041 gcgatcgcgt tctggctgcc cttgaggaag caagcccaga tgcgaaacct ttgcggcgac
     8101 aaaaagcatg gcgcttgcat tcttgcaggt agccacacac gctccacatc caatgcactg
     8161 cgctgcatcc atagccagat cagaatctgt tttggcgatg ggcagacagt ttgcatctac
     8221 cgcattaccg gtgttaatcg atacaaatcc gccggcctga ataattttat caaagcccga
     8281 ccgatccact acaagatctt tgatcacagg gaacgccttc gcacgccatg gttcaatgta
     8341 gatcgtatcc ccgctcttga actttcgcat gtgaagctgg cacgttgtgg tccctttttc
     8401 tggaccatgg ggaactccat tgatcatcat gctgcatgcg ccacaaatac cttcgcgaca
     8461 gtcgtgatcg aatgcaatgg gttcctcttt ctgcgaaatc aaacgctcat taagaacatc
     8521 aatcatttca agaaatgaca tgtgttcgtt gacaccttcc gcttctccta ccttgtattc
     8581 gaccatgcgg cccggatcgt ctttgtcttt ctgtctccag acgtgaagag ttaaattcat
     8641 atatggctcc ttacttgtaa cttctcgtgg cgagatgaat gttttcgaat accagaggct
     8701 ctttgttgag ctttggctcc gacttaccgt tgtattccca cgcggccgcg tagcagaatt
     8761 tgtcgtcgtt gcgctttgct tcgccgtctt cctgatactc ttcgcggaag tgaccaccgc
     8821 atgattcgtt tctatgaagt gcatcacggc acatcaattc gccaaattcc aggaagtctg
     8881 ccacgcgccc tgctttttcg atttcaggat tcagtgaaga cccgctgcca ggaacacgca
     8941 cggtcttcca gaattcttcg cgcaattcag ggatgcgtgc aatggctttc tttaagccag
     9001 cttcgtttct tcccattccg caatgatccc agaggatttt ccccagttcc ttatggaagg
     9061 aatccacaga acgagttccg ttgatggaga ggaatttgtt tgtcatctcc gtgacttctg
     9121 cttccgactt tttgaactcg gcgtgatctt ccgggagttt tgccgggccg acctgtgcca
     9181 ggtagttggc aatggtgtaa ggcaggatga agtatccgtc cgcaagcccc tgcatgagtg
     9241 cactcgcacc cagacgattg gcgccgtgat cagagaagtt tgcttccccg attacaaaaa
     9301 gcccgggcag gttactcatc aaattgtagt ccacccagag tccacccatt gtatagtgaa
     9361 ccgcaggata gattctcatg ggacgcttgt acggatcttc acccgttatg cgctcataca
     9421 tttgaaagag gttcccatag cgttcacgga ttgtgctttc tccgagacgc gcaatggcat
     9481 ctttgaaatc caggtaaaca cccagacctg tatctccaac gccgcgccct tcatcgcata
     9541 cgtattttgc ctgacgcgat gcaacgtcgc gaggtgccag gtttccaaaa ctaggataca
     9601 cgcgttcgag atagtaatct ctttcgtttt caggaatttc agatggatgg cgcttatccc
     9661 ctttgttctt gggggcccac actcgtccgt cgtttcgcag agactccgac atcagagtca
     9721 gcttggattg atagtcaccg cttactggaa tgcaagtcgg atgaatctga gtgtaacatg
     9781 gattcgcgaa aaacgcacct ttcttgtacg cgcgataggt tgcagttaca ttgcatccca
     9841 tggcgtttgt tgaaaggaag aaaacgtttc cgtatccacc gcttgcgatg actacagccg
     9901 cacccgagtg agatgaaatc ttcccggtca caaggtcgcg aactacaatg cctttggctc
     9961 ggccttcgtg cacaaccaca tcgagcattt cagtgcgcgg gtacattttg acggcacctt
    10021 tgccaatctg acggctcaaa gcctggtatg cgccgagcaa aagctgctgg cctgtttgac
    10081 cgcgagcata gaaggtgcgc gaaagctgcg cgccaccaaa cgatctgtta gccaaagtcc
    10141 caccgtactc gcgtgcgaaa ggtacaccct gcgccacaca ctgatcaatg atattcgcac
    10201 ttacctgcgc caggcgatac acgtttgctt cgcgtgcgcg aaaatcgccg cctttgatcg
    10261 tatcgtagaa gagacgcatg gtgctgtcgc cgtcattctg gtagttcttc gcagcgttga
    10321 tcccgccttg agcagcgatg gagtgtgcgc gacgtggact atcctggaag cagaagactt
    10381 taacattgta tccgagttcc gccatcgtcg ctgcagcaga agctcccgcc agacctgtcc
    10441 ccacaacgat cacatcgaac ttgcgtttgt ttgcaggatt cacaagtttc agatcgaact
    10501 tgtgtttgtc ccatttctcc gccagaggac cggacggaat atttgaattc aaagacatta
    10561 cagttctcct ggaagtttaa tgaagttgag aatcacgcaa gtcggagtga tcaggaatcc
    10621 gagaaagatg acgagcgtgt atgcgactga gaacgtcttg attgctccgc taaacctggc
    10681 ctgattccag cccatggatt ggaagaagct tctagatgcg tggctcagat gcagaaccag
    10741 aacgatctga gcaagcacgt aagaagagta gatgatagga tttctgaaac caagaatgaa
    10801 catggtgtag atatcatgtc taccctgtcc gtctacccca gagaaatact gtggctgcac
    10861 aataccgatc gtgaagtgaa gcagatgata aatcaggtaa gcaagcacaa gcaaccctgt
    10921 aagagccatg gtgcgtgatg caagagttgc ctggatggta tttttgaacg catacgcagt
    10981 aggtcttgct gctcgatttt ctcgagcgag tgaaatgcca gtgtacacat gcagaacaaa
    11041 aaggacgatc agtccaattc gcgcaggcca cagcaactga gggtgcgttt tgagatagtg
    11101 gccgtaagcg ttgaacgcat ctggcccggc aaatacgagg aggttccctg tcatatgagc
    11161 aatgagaaat accatgagca gcgctccgga gatcgccatt aggtattttc gcccgaggga
    11221 actggagaag attctttgtg tgaaagacat gccttgttcc gattagattc tatttctttt
    11281 agaactcgcg cccttggcct atttgtcaag gggtttcggc acgagagcgg cgagggcagg
    11341 aagatccgtc aaattggcgt tttccgttac tgctcaatac aaatgatcgt tgggccggac
    11401 gtattgcaga gcgttgcatt attgaagata gctgtggtgg aagtagaatt gtccgcgccg
    11461 tcgcggccgc tccctgcggt ggatgtccaa cctgtgcaat gacttgcatc gtaaacccac
    11521 gtcgacgcaa gaccggtcca atgtcctcca gccgaactga caaaactgtt tgtcagggca
    11581 aaagtgaaga gtgagttgga accagtcgtg cctaaaatcg taatgccgtc tgatcgtctg
    11641 tattgcttcg aaggccggag aacccagtct atctgcccat ctccgagatt ggcggtcacg
    11701 ctggccctgc gcaaattcgt ttgcgggtcc acgagaaacg ccttgaatga acctgttgca
    11761 cccgcgggct tattcacgtc cgaattgcaa gtagcatctg caccggagat gccgccgaga
    11821 tttgcgcttg cgagcccgtt tgtccgaaat gtatagcaga acgcattgga agcgcatgaa
    11881 ggaaccgccg ccgcactggt tgcagatgaa ctttgaaaaa gagctcgcgc cagaaggagc
    11941 tgcgactccg aatccaggga tgtacgcgga aagcttccag tgcaactgga agacacaatc
    12001 aaaaccagaa caatctggag tttacgcata acgctaaaca tatcaaatcg ctcgaaactt
    12061 gtgcaagcaa taattcctat gcccattttt tgccttatag gatcagccat gaaggcagat
    12121 actcatccgc gcgaagctcc acatgagacc gactcacctg ggcattcagg aatgcacagc
    12181 cgcgatgcga ggtggacaga gtgtatgctg cagtctgcga caaggtgccg ttcgtgtcat
    12241 tgatgcgaaa agcagtggca atgttgcctg ctccggaacc aaaagtatag tagaatctac
    12301 ctcgcacatc tatgcatgat gcttgcggcg cagaattatt cacgctgatt tgctgcagga
    12361 gcaacaacgc cccggtcacc ttattaatcc aatagacgta agccgtaccc gttgaatctg
    12421 tcatgtacag gtactttccg ttgggatgaa tcacgaatgg aatattcaga ttcggcgtag
    12481 cggtctggct gatcaaagtc agagaaccat catcacctaa ctgcagtaag tcgatactgt
    12541 ttgtccccgt aacatatacg aaccttccgt ctggatgaga caccagatat ccgttcccgg
    12601 tagaagtgta gctaccaacc gcggtagaaa gagtacccgc agagctgatg ggggcagttc
    12661 tgatgattga attagcacga tagaacaaga acctggctcc aggactgatg gtaagataac
    12721 tcccggtcgg tgtaccaatg gcaaatgtag atggagcacc aaacacgccc gcagcagttc
    12781 gcgaataaac cagaacattt cctccagtgt cgaccacgta ggtcattggc agagttggat
    12841 gattcactgc tgaatgggaa aatgcgactc cggcagtatt cgtagaaaca gtgccaggca
    12901 ccgggactgc ctcagacact gaaaaggaac gcaatccgcc gggattgatg ttgcgcggaa
    12961 cgtataatac tccgttagtc gaatcgacca ctacattcag ggcgtttact gcggccagag
    13021 tagacgtatt ggtgaaaatc tgagtgagtg cgcctgtcgt cgaatctacc tggaaggccc
    13081 acaggtccgg agaactatat cccacgaggt acaagattcg cggttcccat acggtggttc
    13141 tcgatccatc cgccaggagc agggtttcga gagaatgatt tccctgcgac actggctggc
    13201 aactaagccc aaaggcgagc gctaagaatg tgagaatgcc tggtcgcatt tcaagtagct
    13261 tagacatcaa aaatcgcgca cctatgacaa agaagcgcaa aaaaaaaaca cgcgatacca
    13321 gtcagttcct gctccttcgc aatttcaaaa tatactatgc agagcggatt aaaggcaaaa
    13381 accaatcgct tagactaacc gcgcgaagga gccggctccg aagaagaaat ccttgaagat
    13441 caaccaaatt tcgcctatcg caaccttcct tctgggcgct cttttcttta ccaccaatgg
    13501 atgcgcgcgc catctgtcac atggcattga agattggccc ttcctgattg cttactacga
    13561 tatcagttac caggaccttt gcgcgccgtg ggtaacggaa ttttcagtca ttcaacccgg
    13621 aatggccgat cgcgtgcaaa gctatctgca atatcaagct tccctcggct ccgcgagctc
    13681 tgtcggatca acgcgtctat ttggtacagt ctatagcggg aatgcgaagt gggaaggcgg
    13741 agcattggcg cctaacggta agatctacgg cgcacgtttt ggtgcggggc ccacaatgcc
    13801 ttttcttgaa attgatccca ataccctgac cacggccctc gttgggccgg cctccaatac
    13861 aggttctgga atggtgcttc tccccagtgg actattactc gagattccgc atactggagt
    13921 tatgaatcct gtcgttctca atccgaatac aggcgcaacc accacactga gcggatcaaa
    13981 cggaggtgtg gcatgctccg ggggagtgct cgctccaaac ggcaaagtct attgcacagc
    14041 gcagtccgga acgtcaggcg cgcttgaaat cgatccagtc tcaggctccg tgacaatttt
    14101 tggagcagcg caattgggac tgaactacgg gggcagcgct gtgctgggcc tcaatggaaa
    14161 gatttacgca gttccgtatg ctggaacaac tcaaatcgct gaaattgatc cgactacgcg
    14221 cggcatcact ctattcggac catccctgac agcgaacaac tattatggag gaattcttgc
    14281 gccttccggt aagatctatc tgcccgggtt caccacttcc accattctcg aagtggaccc
    14341 gatagcacac acagtgacca gttttggaaa tacaggtgga tcgtccaact acctgggcgc
    14401 cgcagtggcg cctaacggtc acatctacct cactccaaga agctctgctg tagtgcttga
    14461 atttg
//
LOCUS       DAOQJL010000021        14260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2052_length_14260_cov_4.404013, whole genome shotgun sequence.
ACCESSION   DAOQJL010000021 DAOQJL010000000
VERSION     DAOQJL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_14260_cov_4.404013"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLG78_01690"
     CDS             complement(<1..302)
                     /locus_tag="PLG78_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21845.1"
                     /translation="MAILPFASTGANESLAFTEQDLPNLLAGATHFIFESTREHRLQD
                     QAQTEKAVKSSGWTADQPLDSAALRSLCRTTSASRLFAGNAQFLSEGRVAISANVFS"
     gene            295..759
                     /locus_tag="PLG78_01695"
     CDS             295..759
                     /locus_tag="PLG78_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21846.1"
                     /translation="MAISDPAVSAPGKILMQIAKKALIFILLGINSYCRSYPEPDEMQ
                     EYYFYGKYTQLHGLARVPARTIDKEKATVTTLGERRNACREEAIRDAQARFLALTLVQ
                     PLDASEWHRRLNMGAKGEWWGCFERARVRNLFFEYPDYCRVVLVIPCEPGDY"
     gene            759..1802
                     /locus_tag="PLG78_01700"
     CDS             759..1802
                     /locus_tag="PLG78_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21847.1"
                     /translation="MMRWLCLISFLCLASTLSAQVSPVPVSDYAFRPDNDEGYIQAWN
                     VDVRTPNAFLYVTLLVSNVGPGDLNNGVSVLVVKPGSSRIWTAEYSVRSLQATPGQFG
                     HRSGLSRIHRDEKGRIILHSEIPGGSPSPKESLNLDLVLTPVDGGLRISGGLLEPEKG
                     SFLRADLPVIAAKAEGTLTIGGVQEPLSGIAGLDAIWSNKSPHTYAKRFLLIRSFSEA
                     PGIFIGGFMGKEENFLLRFAVAEKGKVKSSGNVKSLEVNASAIDPFSGYSIPTSVTYG
                     LEDGCKITEVRKGFAGGFDVLGSISTVLRWILRVFFARPYVMHFTSQMTYICNGAKDI
                     SAEAQASYYLINR"
     gene            complement(1805..2542)
                     /locus_tag="PLG78_01705"
     CDS             complement(1805..2542)
                     /locus_tag="PLG78_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE21848.1"
                     /translation="MTWILLPLVGIASGIVAGILGVGGGTILVPAMVFLGIEAHSAIG
                     TSTLAMIFTSLSGTIRNDAKGGLQVAAVIQIGIASLLGAQGGAFLTTLLKTRTLEILF
                     AILLIFITISGLRKTNIEKRSPTKWLVAGRALTGLTGGFLSGLFGIGGGIVMVPLQIL
                     LLGEDLITAVKTSLGVIVITALGATIAHAMHGQVLVLEGITLGAGGVIGAQIGTRMLP
                     ALPQSTVKIIFAILATILASALIFWGK"
     gene            2835..3074
                     /locus_tag="PLG78_01710"
     CDS             2835..3074
                     /locus_tag="PLG78_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNE21849.1"
                     /translation="MIPLSDLPIGHSGKIARVAGSSETFQRLRDLGLIEGEVVRMLKR
                     APLGDPIEVRIMNYNLCLRLSEAREIQVKRNQSEK"
     gene            3071..5251
                     /gene="feoB"
                     /locus_tag="PLG78_01715"
     CDS             3071..5251
                     /gene="feoB"
                     /locus_tag="PLG78_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNE21850.1"
                     /translation="MKTLRKRPLIALVGNPNTGKTTVFNALCGTRQHVGNYPGVTVEK
                     KIGTAVLAGASFDVLDLPGLYSLKAAAPDEQLASDALMGRIEGEDRPDLIVYVADATN
                     LKRNLLVYSQLVELDIPVIIALTMTDLLSETGVDLNMSALEKRLKIPIVPVIGKDLET
                     VEVLKSRITSALKEKPHAQADLVYPRKLEKVVSVLNDRLKEFIPLSKFETRNVILFPG
                     DPLLAAFRGKAAALKAIEDARQEVKDIDPGTVTTERYRWAEQIVSQVEKRKEQTGVSF
                     SDKLDKILTHKVLGLAAFVGVMFLVFQSIYTWASPVMDAISGAFDWLGAQSAIVLSSS
                     PMLSSLVADGIIGGVGSVVVFLPQIILLFTFIAILEDSGYLARAAFLMDRLLAWSGLN
                     GRAFIPMLSSFACAVPGVMASRVIPDARARMTTILVSPLMSCSARLPVYLLLIGAFIE
                     PKFGAGWAAFTLFAMHAMGLLIALPIAWVLNRGLLKTPAVPFVLEMPPYRMPHYRNVF
                     YRVYDAARRFLVRAGTVIFAMSIVIWALSYFPRPASIQEQADAKAAALVETESKKGPV
                     SEERKTEIQTATQFEADSLYLGQSYLGRAGKFIQPVFAPLGFDWKITVGVLAAFPARE
                     VIISALGIIYSVGKTDTEQTDSLKERMALEKRADGSTLFTPLLAVSLMVFFALCCQCM
                     STVVTVQRELQSWGWAGFMFAYMTGLAYLFALLIYQGGRAFGLS"
     gene            complement(5221..5646)
                     /locus_tag="PLG78_01720"
     CDS             complement(5221..5646)
                     /locus_tag="PLG78_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21851.1"
                     /translation="MPAVSRILVRTSFCYLILGSAIGTWLLLDKAASTPILEGVRNIH
                     REIMLAGWIVQLVIGTATWILPRNDRSRGDMKSVWIAYALFNAGILVAAIGTILGNQA
                     VLTAAHCCYAAGAISFAKHAWPRIKSLPQDQERPNARPP"
     gene            complement(5646..6056)
                     /locus_tag="PLG78_01725"
     CDS             complement(5646..6056)
                     /locus_tag="PLG78_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21852.1"
                     /translation="MPRLTRYFVKSAFICLVLSFMTGIILHLDFLIPNAGVLRPVFYH
                     LFLVGFTTQLIFGISYWMFPTLSRDNLRGNESLGWISYVALNGGLLLRATAEPAVALS
                     PVPALRITLAAAAFLQLIAVVAYVALIWKRTKAK"
     gene            6104..7300
                     /locus_tag="PLG78_01730"
     CDS             6104..7300
                     /locus_tag="PLG78_01730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNE21853.1"
                     /translation="MNSNPRIVLQPGREKSIKQRHPLVFSGAVAKWPTFEDGDLLNVQ
                     SHSGEYLGCGQFRKGASLAGRMLWFAESPEDTDFQQWAVDRFVAALGLRTSLPLDGNA
                     WRAVAGESDGMPGVTLDRYAEYWVIQISSVGMERNKEHLISAFRSASAKAGVKCKGMY
                     QKVVPRTSAEPGTLESSAWIYGSGVDSVTIQENGLSFRVEFEGSQKTGFFLDQRENRA
                     YLRTLSRNRRVLNCFSYTGGFSVYAASGGASRVVSVDSSEKAIALAKENFSLNQISQA
                     NHGFEASDVFEYLRSVEEEFDIIVLDPPAFARTKRDLPQAMRGYRDIHRLAFQVIEKG
                     GLVLTSSCSHPVDSDLMRKIIFQAALEARRDVRILQSHRQSPDHPLSIFYPEGDYLKG
                     FLLHID"
     gene            complement(7301..7981)
                     /locus_tag="PLG78_01735"
     CDS             complement(7301..7981)
                     /locus_tag="PLG78_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21854.1"
                     /translation="MTSKSVCGLAIAVIFLPFLGDCATQFSPTTLSIVSDKNLEQMEK
                     PVLLASATQSQCRVVLQQRVGYVLFHLPFNEITSGQIKSALQSQKEPYSIRYREMLTP
                     ADIGLNILGFLFSVLTVTKQVEACAAPEMVESSDQAKKARIYSEFEKERKRFSRFEVP
                     IGLTTTIEKKVRHRVYQKPEDGEAAMISREGSLTIKSNKLQLETEEGTVAIPEDSVLM
                     IEVMIPEG"
     gene            8099..9052
                     /locus_tag="PLG78_01740"
     CDS             8099..9052
                     /locus_tag="PLG78_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21855.1"
                     /translation="MRRSIVLAALLVLLTCTSCALGLLQLPGSKSDDDNLSLMLLGIL
                     ALQPAGPRYEFQETTVTPTTANANYYGAGITGYSVVVSGSTVGPFNPSEIFQAEQNHA
                     VSNAYIDFQQRVDSSGWFFSRNAPLSASAQNGSTFHLYGATPNLYTSIATTATASSTQ
                     TLNFAIIAPSNSLPMTYRRIRALIPASSEESNFSPDSTTVNYKLRGMSKVWSTEKRLN
                     VNLIFISGSNPAASTTGIAEAITRMTDLYTQNTVRIRPVISSTTIVLPSYLTLTSLST
                     DTTATGSLGGLMVNTASAQRADALNIIFVKNETAVGGVLGI"
     gene            9080..9451
                     /locus_tag="PLG78_01745"
     CDS             9080..9451
                     /locus_tag="PLG78_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21856.1"
                     /translation="MGTRQSGMVVMFDGHLASAGAVPSSAEQRLIGETMAHEGGHWLG
                     LFHPVESNYDTTQNNYNRDAISETPLCTAAGVGLAACDFTAVNNAGSRNVMFWAGTAG
                     FDQSDLTGEQGFVLRRHPLAY"
     gene            9459..9998
                     /locus_tag="PLG78_01750"
     CDS             9459..9998
                     /locus_tag="PLG78_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21857.1"
                     /translation="MNNNYKMPRLIFAGLFFFGFVSAAHSQTLDPGTMDRLRTALGQV
                     RHPDQVDMLQMAQSIAPVNTDAYLIALASQPTSVPGLRLRAYGLLGHFSASPATKAFL
                     AQQVTRRELDDSYRQWALTSYVRAFHDVDPAGVEIVVSSAERDPSLNLREHARVIHQK
                     MRAGARHPAGELPRRQKAR"
     gene            complement(10013..10600)
                     /locus_tag="PLG78_01755"
     CDS             complement(10013..10600)
                     /locus_tag="PLG78_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNE21858.1"
                     /translation="MKNLLKHDGVAVYHGVVFPSETSDAYFGSLLSEIEWKNDEAIMF
                     GRHIITARKVAWYANQAFSYTYSRKTRHALPWSPALRELKQIVEDRTGYTFNSCLCNL
                     YHNGNEGVTWHSDDEKSLKKHAAIASLSFGAERVFQFKHKKSGDIVSILLEHGSLLIM
                     SGATQTNWKHRLPISKKILEPRINLTFRTMEHQAT"
     gene            complement(10704..11576)
                     /locus_tag="PLG78_01760"
     CDS             complement(10704..11576)
                     /locus_tag="PLG78_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE21859.1"
                     /translation="MIRLISGLILTLFVAACSTPGQARFEPQGAQTVAKTKTVIFIHG
                     MFMTPASWANWQKYFESRGYKTLAPAWPYHEGKPSDQRASHPNAQLGKLTLDDVLESY
                     RKVIREQKEKPILIGHSMGGLIVQLLMQEGLGAAGIAIDSAPPKGLISLKFSFLTSNW
                     GVISPFANKEEPLLLSPEQFNYAFVHTLTESEQKSVYDSFVVPESRLVGNGPTTDTAA
                     IDFSKGRLPLLMIAGELDHITPASMNYSNFQKYESSPSKTEFRMFNGRTHWIAGQKGW
                     EEVADFVLEWINRN"
     gene            complement(11671..13251)
                     /locus_tag="PLG78_01765"
     CDS             complement(11671..13251)
                     /locus_tag="PLG78_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE21860.1"
                     /translation="MCNPRRPSRFWVTLLDQTQLKLPEVIAALSLATDLANDNPPETT
                     LRICYIALEIAREAGLRPRELEDLYYGALLRFIGCTAYAHEEAVAMGDDVAARHSLAR
                     VDVSRPSRVLGTALRSSKGKSKPAVLWNFLTQSGTMFSNFTAANCEVACMLGQKIGMP
                     EGVLRVLEQIHERWDGKGGPSRVRGNTLLASVRILHVANTAEIIRQAAGTDAACAVIQ
                     DRSGEQFDPDVCKGFLRIGRDLLHSMETVSVWDEVIRRAPRSESSPGGARLRKIAEAF
                     GSFVDLKSVYTAGRSLNISNTVERAAQWMGLKDTEDLVIASLLADIGMVSVPTNLLEK
                     KGKLNPSEWERVRLHTYYTERILTRSPVLARIAPIAGAHHERLDASGYHRGSSGPSIP
                     QGGKLLAAADLYHALLERRSYRAPVAPSSAAQMLREEVARGRIDRTCADAVLAVTNHT
                     AISSSPGAESTSTLSPRECEVLTLLASGFSNKEIGAKLNISHRTVQHHVEHIYGKISV
                     STRAAATLYAVQNGFLRA"
     gene            13434..13736
                     /locus_tag="PLG78_01770"
     CDS             13434..13736
                     /locus_tag="PLG78_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE21861.1"
                     /translation="MVGLLRNKPLSISDISRPFRMSFAGVAKHVDVLEGAGIVRKVRD
                     PEDRRVFIIQLQREPLDEAVDWLEYHREFWSGKLDSLEAFMEEQDGNADRKNRKKN"
     gene            13705..14127
                     /locus_tag="PLG78_01775"
     CDS             13705..14127
                     /locus_tag="PLG78_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE21862.1"
                     /translation="MQIVKIEKRIKADCKRLFRAWLDPSDFSRWFLPGEKVGIGNVTL
                     DPQPGGYFKIDMMVNGQVRPHEGSYVEITEPSKLVFTWKSWKTGGRDTLVTITFVPEG
                     DTTLITLIHEQLPDAEACESHTHGWTGILTGLAAFFEK"
BASE COUNT         3297 a         3594 c         3793 g         3576 t
ORIGIN      
        1 gaaaacacat tcgcggaaat ggccactctc ccttccgaca gaaactgggc gttcccggcg
       61 aagagcctgg atgcggaagt ggttctgcaa agactgcgaa gcgccgctga atcgagaggc
      121 tggtctgcag tccaccctga gcttttcact gccttttctg tctgggcctg gtcctgaagg
      181 cgatgttccc gcgtggattc gaaaataaag tgagtggccc ccgcaagcag gttcggcaaa
      241 tcctgctccg tgaaggcgag tgactcgttt gctccggtgg aagcaaaagg caaaatggcc
      301 atttcagatc cggccgtgag tgcgccgggc aagatactga tgcagattgc gaagaaggct
      361 ttgattttca tcttactggg aatcaactct tactgtcggt cctatcccga accggatgag
      421 atgcaagaat attacttcta tggaaagtac actcaacttc acggtctggc cagagtgccg
      481 gcccgtacga tcgacaagga aaaggccacg gtgactaccc tgggcgaaag gagaaatgca
      541 tgccgcgagg aagccatccg ggatgcccag gcacgttttc ttgctttgac tctggttcag
      601 ccactcgatg cgtcagaatg gcatagacga ctgaacatgg gagcgaaagg agagtggtgg
      661 ggctgctttg agcgcgcgcg tgtgcgaaat ctttttttcg agtatcctga ttattgcagg
      721 gttgtcctgg ttatcccctg cgaacctgga gattactgat gatgcgctgg ttgtgtctga
      781 tttcgtttct gtgccttgcg tccactctct cggcgcaggt ttctcccgtt cctgtttcag
      841 actatgcatt tcgcccggat aacgacgaag gctacattca ggcatggaat gtagatgttc
      901 gcacgccaaa tgcatttcta tatgttaccc ttcttgtttc caatgttggc ccgggggact
      961 taaacaacgg cgtttctgtt ctggtagtaa aaccgggttc cagtagaatc tggactgcgg
     1021 aatattctgt gcgaagtctc caggcaaccc ccggtcagtt cgggcataga agcgggctca
     1081 gcagaattca cagggacgag aaaggtcgaa tcatcttaca ctctgagatc ccgggaggaa
     1141 gcccgtcgcc caaagagtcg ctcaacctgg atttagttct gaccccggtg gacggcggtc
     1201 ttcgcatttc aggtggtctt ctcgaacccg aaaaaggatc tttcctgcgc gcggatcttc
     1261 ctgtaatcgc cgcaaaggca gaaggcacat tgacgatagg cggcgttcag gaaccgttaa
     1321 gcggcattgc tggcctggat gccatctggt caaacaaatc gccacatacg tatgcgaaac
     1381 ggtttcttct gatccgatct ttttcagaag caccaggaat cttcatcgga ggattcatgg
     1441 gcaaagagga aaacttctta ttgaggtttg cagtggctga aaagggaaag gtcaaatcgt
     1501 ctggaaatgt aaagtccctg gaagtcaatg catctgcgat cgatccattc tccggatatt
     1561 ctattcccac atctgtcacc tacggcctcg aagatggctg caagattacg gaagtgcgca
     1621 aaggctttgc cggaggattc gatgttctcg gaagcatttc gactgtgctg cgctggatcc
     1681 ttcgtgtctt cttcgcgagg ccttacgtaa tgcacttcac ttcgcagatg acgtacattt
     1741 gcaacggagc caaagacatt tctgcagagg cgcaggcgtc gtattactta atcaatcgct
     1801 gatcttattt tccccagaag ataagggcag acgcgagtat agtcgcgaga atggcgaaaa
     1861 tgatcttcac agtgctttgt ggcaatgccg ggagcattct cgttccgatt tgggcaccaa
     1921 tgactcctcc cgcgcccaga gtgatccctt cgagaacaag aacctgaccg tgcatcgcgt
     1981 gcgcgatcgt tgcgcccaga gcagtgatga cgatgactcc cagacttgtc ttgaccgctg
     2041 tgatgagatc ttctccgaga agtaaaattt gcaatggcac catcacgatt ccgcctccaa
     2101 ttccgaaaag accggagagg aaaccacccg tcagtcccgt cagagctctt cccgcaacga
     2161 gccacttcgt gggtgagcgc ttttctatgt ttgtctttcg cagtccagag attgtaatga
     2221 agatgagcaa gatcgcgaac agtatttcca gcgtgcgtgt tttgagtaat gtagtgagga
     2281 acgctccgcc ctgcgccccg agtagagaag cgatcccgat ctggattacg gctgcgacct
     2341 gcaacccgcc cttcgcatca ttgcgaattg tcccggaaag cgatgtgaag atcatggcaa
     2401 gggtgcttgt tccaatggca ctgtgtgcct caattccaag aaacaccatc gcaggaacta
     2461 gaatcgtgcc gccaccgacg cccagaatgc cggccacgat tccggatgca atgccaacga
     2521 gcggcaagag gatccaggtc atggcccagg ctgcaccggg cgcctgtgcg ccatacttca
     2581 attcctggcg atctggtctg gattttgaga ctgggtctca gaaacgaatg gacgattgtt
     2641 gtgggccgtg agtgatggca tgaagaggaa aacatggaag tagttgtggt cacactgttg
     2701 atctctctgg cgggcgcgta tttgatctgg aaggctgctg ccttcttccg tagaaaaccg
     2761 gcgggcgcag gtcccgcgat ccatcaagat tgctccggct gctcctgtag cgccggagat
     2821 tgcaggaaag acgcatgatt ccactttctg accttcccat tggccactca ggcaaaatcg
     2881 cccgcgtggc gggttcgagc gaaacattcc agagactccg cgatctgggt ctcatcgaag
     2941 gcgaagtagt tcgcatgctc aaacgcgcgc cgcttggaga tcctattgaa gtgcggatca
     3001 tgaattacaa tttgtgtctt cgtcttagcg aggcgcgcga aatccaggta aaaagaaatc
     3061 agagtgaaaa atgaaaacgt tgcgcaagag accgctcatc gcccttgtag gaaaccctaa
     3121 caccggtaag acgactgtat tcaatgcact gtgcggcacg agacagcacg tgggcaatta
     3181 tcccggcgtc acagtggaaa aaaaaatcgg aacggctgtg cttgccggtg catcgttcga
     3241 tgtgctcgat cttccgggtc tgtacagtct gaaagcggcc gcgcctgatg aacagcttgc
     3301 gtcagacgcg ctcatgggta gaattgaagg ggaagacagg cctgatctca ttgtctatgt
     3361 tgcagatgcg accaatctca aacgcaattt actcgtttac agccagctcg tcgaactgga
     3421 catccccgtc atcatcgcat tgaccatgac cgatttgctc tccgagacgg gagtggatct
     3481 gaatatgagt gcactggaaa agagactcaa gattcctatc gtccctgtca taggaaagga
     3541 ccttgagaca gtcgaggttc ttaaatcgcg cataacgtcc gcgctcaaag agaaaccgca
     3601 tgcacaggca gatctagttt atccccgcaa acttgagaaa gtagtttctg ttctaaatga
     3661 tcgactcaaa gaattcattc cgctcagcaa atttgaaacg cgaaatgtga ttctttttcc
     3721 cggcgatcca ctcctggccg cctttcgcgg aaaagcagcg gctctcaagg ccattgaaga
     3781 cgcacgtcag gaagtaaaag acattgatcc tggaactgta accaccgagc ggtatcgctg
     3841 ggcggaacag atcgtaagcc aggtagaaaa gcgcaaagaa caaacaggcg tttcgttctc
     3901 tgacaaactg gacaagattc tcactcacaa agtactcggt ctggctgcgt tcgtcggagt
     3961 gatgtttctg gtgttccagt ccatctacac atgggccagt ccggtgatgg atgcgatatc
     4021 tggcgccttc gactggttag gtgctcaaag cgcgatcgtg ctttcttcat cgcccatgct
     4081 ttccagtctg gtggcagatg ggatcattgg tggcgttggg tctgtagttg tatttctgcc
     4141 tcagatcatt ctgcttttca cattcattgc tattctcgaa gattccggtt atctggcgcg
     4201 cgctgctttc ttgatggaca ggctccttgc gtggtccggc ctcaacggac gcgctttcat
     4261 tcccatgctt tccagttttg catgtgcagt acccggagtg atggcttctc gcgtgattcc
     4321 ggacgccaga gccagaatga ctacaatcct tgtgtctcct ctgatgtcct gttctgcaag
     4381 acttcctgtc taccttttgt tgattggagc ttttattgaa ccgaagtttg gtgcgggctg
     4441 ggcggcgttc acgttattcg ccatgcacgc catgggtctg ttgatcgcgc tacccatcgc
     4501 gtgggtctta aatcgaggat tgctcaaaac acctgcggtt ccttttgttc tggaaatgcc
     4561 tccgtatcgc atgcctcact atcgcaacgt attctatcgt gtgtatgacg cagcacgcag
     4621 attcctcgtt cgcgcaggca ctgtgatctt tgcgatgtcc attgtgatct gggccctgag
     4681 ttactttccg cgtccggctt ctattcagga gcaagcggat gcgaaggcgg cagctctggt
     4741 agaaacagaa tcgaagaaag ggccagtaag cgaagagaga aaaactgaaa tccagaccgc
     4801 aacgcaattc gaagcggact ctctctatct gggacagagt tatctgggcc gggccggcaa
     4861 attcattcag cctgtatttg cgcctcttgg ctttgactgg aagattactg ttggcgtgct
     4921 ggccgctttt cctgcgcgcg aagtgatcat ttctgcactt ggaatcatct acagtgttgg
     4981 aaagacagac accgagcaaa cggactctct caaggagcga atggcactcg aaaagagagc
     5041 ggatggttct actttgttta ctcctctcct tgctgtttcg ctcatggttt tcttcgccct
     5101 ctgttgtcag tgcatgagta ccgttgtaac tgttcagaga gaattgcaga gctggggatg
     5161 ggccggattc atgttcgctt atatgactgg ccttgcatat ttgttcgctc tcctgatcta
     5221 tcagggtggg cgagcgtttg gtctttcttg atcctgcggc aagcttttga ttcgcggcca
     5281 tgcatgcttc gcaaaagaaa tagcccccgc cgcatagcaa cagtgtgccg ccgtcaggac
     5341 ggcttgattt ccaagaatag ttccgattgc cgccaccagg atgccagcgt tgaacagcgc
     5401 gtaggcgatc catacacttt tcatatcgcc gcgactccgg tcatttctcg gtaagatcca
     5461 ggtggctgtt ccgatcacaa gctgaacaat ccacccggcc agcatgattt cgcggtggat
     5521 gtttcgcacg ccctcgagga ttggagtgga agcagcttta tcgagcaaga gccaggttcc
     5581 aatcgcagag cccagaatca gataacagaa agatgtgcga acgaggatac gactgacggc
     5641 tggcattatt ttgccttcgt cctcttccag atcaaagcaa cgtaagcaac cacggcgatg
     5701 agttgcaaga acgcggccgc ggccagagtg attcgcagcg ccggcaccgg agaaagtgct
     5761 actgccggtt cagccgtggc gcgaagcagc aagcctccgt tcagagcgac gtaagaaatc
     5821 catccgagcg attcgttgcc ccttagattg tctcgcgaaa gagtgggaaa catccagtac
     5881 gagattccga aaatcagctg agttgtgaat cccaccagga atagatgata aaaaacaggg
     5941 cgaagcacac ctgcgtttgg aatcagaaag tccagatgga gaatgattcc ggtcatgaaa
     6001 gacagaacca ggcagatgaa cgcggatttt acaaaatagc gagtcaatct gggcatcaca
     6061 cgcattcgtt tgacgtgcga gcgttgtcca gctctttgtt ccagtgaaca gcaatccgcg
     6121 catcgtgctt caacccggca gagagaagtc gatcaaacaa aggcatcctc tggttttttc
     6181 gggtgcggtg gcaaagtggc ctacattcga agatggggac cttctaaacg tacaaagtca
     6241 ttccggcgag tatctgggat gtggccaatt tcgcaagggt gcttctcttg ccggccggat
     6301 gctctggttt gcagaatctc cggaggacac tgattttcag cagtgggccg tggatcgctt
     6361 tgtggcggcc ctgggcctgc gcacttcttt accgttagac ggcaatgcgt ggagggctgt
     6421 ggctggagaa tcagacggta tgccaggagt cactctggac cgttatgcgg aatattgggt
     6481 cattcagatc tcatccgtcg ggatggagcg aaacaaagag catctcatat ctgcgtttcg
     6541 atctgcatcc gcgaaagctg gagtaaagtg caaaggcatg tatcaaaagg ttgtgccgcg
     6601 tacttctgcc gaacctggaa cactggagtc aagcgcctgg atttatggga gcggtgtgga
     6661 ctctgtaacg attcaggaaa acggtctttc ctttcgcgtt gaatttgagg gatcccagaa
     6721 gacaggcttt ttcctggatc aaagagagaa ccgcgcctac cttcgcactc tttctagaaa
     6781 taggcgggtc ttgaattgct ttagctatac gggcggcttc agcgtgtatg ctgcatccgg
     6841 tggagcatct cgcgttgtct ccgtagatag ttcagaaaaa gcaattgctc tggcaaagga
     6901 gaacttttct ctcaatcaga tttcgcaggc caatcacgga tttgaggctt ccgatgtctt
     6961 tgaatacttg cggtccgtgg aagaagaatt tgacatcatc gtccttgatc cacctgcctt
     7021 tgctcgcacg aagagagatc ttcctcaggc aatgcgcggg tatcgcgaca tccacaggct
     7081 tgcatttcag gtcatcgaaa aaggaggtct ggttctcacc agttcttgct ctcatcctgt
     7141 ggacagtgat ctcatgagaa aaatcatttt ccaggccgca ctggaggcaa ggagggatgt
     7201 tcggatcctt caatcccatc gccagagccc ggaccatcct ttaagcatct tctaccccga
     7261 aggtgactac ctcaaaggat tcttacttca cattgattga ttagccttcc ggaatcatga
     7321 cctctatcat gagaacggaa tcttcgggga ttgccactgt accctcttcc gtttcaagtt
     7381 gaagtttgtt ggacttgatc gtgagcgatc cttccctgga gatcatagct gcttctccgt
     7441 cctctggttt ttgataaact ctgtggcgga cttttttttc gattgttgtg gtaagaccga
     7501 tgggcacttc aaaccgcgag aacctttttc tttccttctc aaattctgag tagattcttg
     7561 ctttttttgc ctggtcggag ctttccacca tctcaggtgc tgcgcatgct tctacttgtt
     7621 tggtcacggt cagcacgcta aatagaaaac cgaggatgtt cagaccaata tctgccggcg
     7681 tgagcatttc cctgtagcga atggaatatg gttccttttg cgattgaagt gcggatttga
     7741 tttgtccgct tgtgatttca ttgaacggca agtggaagag tacgtatcct actctttgct
     7801 gcagcacaac gcgacattga gattgtgtcg cggaagcaag aagaacgggc ttctccattt
     7861 gctccaggtt tttgtcagat acaatggaca gggttgtcgg ggagaattga gtagcgcagt
     7921 ctccgagaaa gggaaggaat ataacagcga tcgctagccc gcagacggat tttgaggtca
     7981 ttcttcagtc gggatgccca cgggacggag tcaagattca ttttagaaag tttatggttt
     8041 tttagcgagt ggttccattg tgtgttgact ggtcgggggc tgtccttcag catgaggcat
     8101 gcgtcgatcg attgtgctgg ccgccctgct tgtattgctc acctgcacat cctgtgctct
     8161 tggtttgcta caattacctg gttccaaatc agatgatgac aatctcagtc tgatgctgct
     8221 ggggattctg gctctgcagc ctgccgggcc cagatacgaa ttccaggaaa caacggtgac
     8281 gccgactacg gcgaatgcaa actactatgg tgcggggatt accgggtatt ccgtggttgt
     8341 gtctggatcc acagtcggcc cattcaatcc atctgagatt tttcaggcgg aacagaatca
     8401 tgcagtgtcc aatgcttaca ttgactttca gcagcgtgta gatagcagcg gatggttttt
     8461 ctcccgaaac gcgcctctgt ctgcctctgc gcaaaacgga agtacatttc atctctacgg
     8521 agccacaccg aacctttata cttctattgc cacaaccgcg acggcttcca gtacgcagac
     8581 cttgaatttc gcaatcattg ctccaagcaa ttcgcttccc atgacatatc gccggattcg
     8641 cgctctgatt ccagcttcgt cggaagaaag taatttctca ccggattcta caacagtcaa
     8701 ttacaagctt cgcggaatga gcaaagtctg gtccacagag aagcgtctca atgtaaattt
     8761 gattttcatc tctggttcca atcctgccgc gtccacgacc gggatcgcgg aagcgattac
     8821 tcggatgacg gatctataca ctcaaaacac agttaggatt cggccggtca tctcttcaac
     8881 gacgattgtg ttaccgtcct atctcactct cacaagtttg agtacggata caactgcaac
     8941 cggcagtctc ggaggcttga tggtaaacac tgcatcggct cagcgtgcag acgctttgaa
     9001 tatcatattc gtgaaaaacg aaactgcggt tggcggcgtt ctgggcatat aaactggaat
     9061 tcccgggcta ccaggaagag tgggaaccag gcagtcggga atggttgtta tgttcgacgg
     9121 gcatctggca agtgcgggcg ccgtaccgag ttctgcggag caaaggttga tcggcgaaac
     9181 catggcccat gagggcggtc actggctcgg gctttttcat ccagtagaga gcaactatga
     9241 cactacgcag aataactaca atcgcgatgc gatttctgaa acacctttgt gtacggccgc
     9301 gggtgtgggt ctcgctgctt gcgattttac cgctgtgaac aatgcgggat cgcgcaatgt
     9361 tatgttctgg gccggcacgg ctggatttga ccaatcagat ctgacggggg agcagggttt
     9421 tgtgcttcgc agacatccgc ttgcgtattg aggtgaacat gaacaacaat tacaaaatgc
     9481 cccgcttgat cttcgcgggt ctctttttct ttggatttgt ttctgccgct cacagccaga
     9541 cactcgatcc tggcacaatg gacagactga ggaccgcact cgggcaagtt cgccatccag
     9601 atcaggtgga tatgttgcag atggcgcagt ccatcgcccc cgtaaatacg gatgcgtatc
     9661 tgatcgcgct tgccagtcag cccacgagcg ttcccggatt gcgtttgcgc gcctatgggt
     9721 tgctcggaca tttctctgca agtccagcca cgaaagcctt ccttgcgcag caagttactc
     9781 gaagagagtt ggatgattct tatcgccaat gggctcttac cagctatgtg cgtgcatttc
     9841 acgatgtgga tcctgcggga gtggaaatcg ttgtgtcctc tgcggaacgc gatccgtcct
     9901 taaatctgcg tgagcatgcc cgggtcatcc atcagaagat gagggcagga gccagacatc
     9961 cagcaggaga gctgccgcgt cgtcagaaag cacgttaggc gattttgatt cctcacgtag
    10021 cttgatgttc catagtgcga aatgtcaggt tgatcctggg ctccaggatt ttctttgaaa
    10081 ttggcaatcg gtgtttccaa tttgtctgtg tggcaccgct catgatcaaa agacttccgt
    10141 gctccagcag aatcgagaca atgtcccctg atttcttgtg tttgaattga aataccctct
    10201 ctgcgccaaa gctgagagat gcaatcgcag cgtgtttctt taacgacttt tcatcgtcgc
    10261 tgtgccaagt gactccttcg ttgccgttgt gatacagatt gcacaggcag gaattgaatg
    10321 tgtaaccagt tcgatcctct acaatttgtt ttaattcgcg gagcgccggt gaccagggca
    10381 gagcatggcg agtctttcgt gaatatgtat acgagaatgc ctggttcgcg taccaggcga
    10441 ctttgcgtgc tgtgatgatg tgccggccaa acatgatggc ttcatcgttc ttccattcaa
    10501 tttcagacag aagcgagcca aaatatgcat cgctcgtctc tgatggaaaa accacaccat
    10561 gatagacggc cacaccatcg tgcttgagta aattcttcat ttttcacgcg atttcggaac
    10621 tacaggcttc ctgcggatca ccattctctc tttcgggttt ggttccgcag gtcttgtcgc
    10681 cctaccggcc agcaaacata ccgttagttg cgattgatcc attcgaggac gaaatcggca
    10741 acttcttccc agcctttctg gcccgcgatc cagtgtgtgc ggccgttgaa cattctgaat
    10801 tcagttttgg aaggcgagct ttcatatttc tgaaaattag aatagttcat ggatgcaggc
    10861 gtaatgtgat cgagttctcc tgcaatcatc agtagaggca gcctaccttt cgagaaatcg
    10921 atggcagccg tgtctgtcgt cgggccattc cccaccagtc tggattccgg tacgacgaat
    10981 gagtcataca ccgatttctg ttctgactca gtcaacgtgt gaacgaacgc atagttaaat
    11041 tgctccggcg acagaagaag cggctcttct ttgttcgcaa atggactgat cacaccccag
    11101 ttgcttgtca aaaacgaaaa tttcagcgat atcagccctt taggtggagc agagtcgatc
    11161 gctatgcccg cagcgcccag gccttcttgc atcagcagct ggacaatcag tccacccatg
    11221 gaatgtccaa tgaggattgg cttttccttt tgctcgcgaa tcacttttct gtaactttcc
    11281 agaacatcgt ctaacgtcag ttttccaagt tgcgcattgg gatgggaagc tcgctgatcc
    11341 gaaggttttc cctcgtgata aggccacgct ggagcgagcg tcttgtagcc tctggattca
    11401 aaatactttt gccagttagc ccagctggcg ggcgtcatga acatcccgtg tatgaaaatc
    11461 accgttttgg tctttgcaac tgtttgtgcg ccctgaggct caaaacgagc ctggcctggc
    11521 gtgctgcagg ctgctacgaa taaggtcagg atcaatcctg atatcaatct aatcatttgt
    11581 ttcctccgcg aaactaccag aatactatca caactgaaga gggcgcgcat cggccatttg
    11641 gcctatgctg ggcaagcgga ccatttctga tcaggcgcgc aggaatccgt tctgcacagc
    11701 gtagagagtg gcggctgccc tggtggagac agagattttg ccgtatatgt gttccacgtg
    11761 gtgttgcacg gttctgtgtg aaatattcaa tttcgcgccg atctctttgt ttgagaatcc
    11821 gctggccagg agagtcagca cttcgcattc gcgcgggctg agcgtggatg ttgattcggc
    11881 gccaggagat gaggaaatgg ccgtgtgatt ggtcactgcg agcaccgcat cggcgcatgt
    11941 cctgtctatt ctgcctctcg ccacttcttc gcgcagcatt tgtgcagcgg aggacggagc
    12001 aacgggcgcg cggtaagatc gacgttccaa aagggcatga tacaaatctg ccgctgccag
    12061 caacttgccg ccttgcggga tggagggacc agaacttcct cgatgatatc cggaggcatc
    12121 caatctttca tgatgcgctc ctgcaatcgg tgcgatgcga gcaagcacag gggagcgtgt
    12181 cagaattctc tccgtatagt aggtatgcag acgcacgcgt tcccactcgg aaggattgag
    12241 tttccctttc ttctcaagca agttcgtggg cacactgacc atgccgatgt ctgcgagtag
    12301 gcttgcaatg accaggtctt ccgtatcctt gagacccatc cattgcgcgg cccgttcgac
    12361 cgtgttactg atgttaagcg agcggcctgc cgtatagacg gacttgagat ctacaaaact
    12421 gccaaacgcc tctgcaattt tgcgcagccg agcacctccg ggtgagcttt cactccgagg
    12481 ggcccttcga atcacctcgt cccagacgga aacagtttcc atagaatgca acaaatcacg
    12541 accaattcgc aggaaacctt tacaaacgtc cggatcaaat tgctccccac ttctgtcctg
    12601 gatgacggca caggccgcat ctgttcctgc tgcctgtcgt atgatctctg cggtgtttgc
    12661 gacgtgcagg attcgcacgg atgccagaag cgtattgccg cgcactctgg acggtccgcc
    12721 ttttccatcc catcgttcgt gaatctgctc gaggacacgc aacaccccct ccggcattcc
    12781 gattttttgc cccagcatgc acgcaacttc acaattggct gcggtaaaat tggagaacat
    12841 agttccggat tgggtgagaa agttccagag cactgcaggt ttcgactttc ccttgctgct
    12901 tcgcaaagca gtccccagta cccgagaagg ccgactaacg tctactctgg ccagggaatg
    12961 tcgcgctgcg acatcatcgc ccatggcgac tgcttcctca tgggcgtatg cagtgcaacc
    13021 gatgaacctg agcaaggcac cgtagtacag gtcttccagc tcacgaggcc tgagaccagc
    13081 ttctcgggcg atttccaggg caatgtagca aatccgcaga gtggtttcgg gtggattgtc
    13141 gtttgccaga tccgttgcca gagacagtgc agcgatgact tcaggtagtt ttagttgagt
    13201 ctggtccagc aaagtgaccc agaaacgcga cggccgtcgg ggattgcaca caattttttt
    13261 cacgaaaggc gatcgtcttt attcaaccaa atggttttgt agttgtcatt tgcggggtgt
    13321 cctatttatt aaaccagatg gttgaattaa aaagaaaggt gcgcccggtg cggtccgcgc
    13381 aacgaaattt ggactccgtg ttttccgccc tcgcggatcc atcgcggaga aaaatggtgg
    13441 ggctgctgcg caataaaccg ctgagcattt ctgacatttc caggccattt cgcatgtcgt
    13501 ttgccggagt tgccaagcac gtggatgtgc tcgaaggtgc gggaattgtt cgcaaggttc
    13561 gagatcccga ggaccgccga gtcttcataa ttcaactgca gagagaaccg ctcgatgagg
    13621 ccgtagactg gctggaatat caccgagagt tctggtccgg taagctggac agtctggaag
    13681 cattcatgga ggaacaggat ggaaatgcag atcgtaaaaa tagaaaaaag aattaaagcg
    13741 gattgtaagc ggctttttag agcgtggctg gatccgtcgg acttttcccg ttggttttta
    13801 ccaggagaaa aggtcggaat cgggaacgtg acgctcgatc cgcaacccgg cggatatttc
    13861 aaaatcgata tgatggtcaa cggtcaggtc cgcccgcacg aaggaagtta tgtggaaatc
    13921 acggaacctt caaagctagt ctttacatgg aaatcgtgga agacaggcgg aagggacact
    13981 ctcgtgacca tcaccttcgt gcccgaaggc gacactacat tgatcactct catacacgaa
    14041 cagctgccgg acgcggaagc ctgcgaatct catacgcacg gttggacagg aatcctcacc
    14101 gggctcgcag catttttcga aaaataagat caaaggaagg aagctatgaa ccggatatat
    14161 cacaaggtcg gcattcaagc cacagcggag aagattttca atgctctgac tacacagtca
    14221 ggtctggcag gttggtggac gaatgaagtg acaggcgctt
//
LOCUS       DAOQJL010000022        14167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2074_length_14167_cov_4.833688, whole genome shotgun sequence.
ACCESSION   DAOQJL010000022 DAOQJL010000000
VERSION     DAOQJL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_14167_cov_4.833688"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLG78_01780"
     CDS             <1..206
                     /locus_tag="PLG78_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE21863.1"
                     /translation="TDGLTEARDRTGKLICSEEDLAERAGDRSRDVSTLCEDIFQDVL
                     SKTGGMDFLEDDITFLIAEYTGS"
     gene            378..959
                     /locus_tag="PLG78_01785"
     CDS             378..959
                     /locus_tag="PLG78_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21864.1"
                     /translation="MFQIVLAGQHTVQDADGEHPLKIFDGNESIMEVSGPFELRGGRL
                     TELFLEFDPNTSVFFTSEGYVLDADIRVASVLSMTPEQENRIVQALGPKTNLVADSAE
                     LALEGQVETTTVAAERIPRTRVSIRIEDRLRGLPANMNALQLTVPGGVVNGLRLRVYG
                     MPQFSQNERVILFLKRIGNEYGIVEGEYGKVSL"
     gene            956..1345
                     /locus_tag="PLG78_01790"
     CDS             956..1345
                     /locus_tag="PLG78_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21865.1"
                     /translation="MKRARWLFFLPCLVSLNFVPVEQGFKFPGDWPKIPFYVNPSVPG
                     ERIGGTDALMNAMRDASAMWSSDVTNVRFDFRVSGFSDRAQPFAPAVLDCSSQTTADF
                     AQTAKTVFAFYACGFEEYDFDSKGNHR"
     gene            1465..1800
                     /locus_tag="PLG78_01795"
     CDS             1465..1800
                     /locus_tag="PLG78_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21866.1"
                     /translation="MIPILRCAHPLGYKVRFSSGAVDCIIPSTSPYIYNQSTTAYAGS
                     TVTGVVTLECRAPTAIKVWSKIATSNPAPGSTATFVVSGNSTLASYSVGDTIFCSDEN
                     LFYHKVTIL"
     gene            2269..2598
                     /locus_tag="PLG78_01800"
     CDS             2269..2598
                     /locus_tag="PLG78_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21867.1"
                     /translation="MGDILFNAASGNNWQRGIARCYTNGSPYQGSDNIVLNVGGNDLL
                     NFYMRKHTYQAEYLFVRYVLNPKGWLKLIFDRGAVDRELEIFWMWSMHLNNFRIALAV
                     ESIGNYI"
     gene            complement(3211..3795)
                     /locus_tag="PLG78_01805"
     CDS             complement(3211..3795)
                     /locus_tag="PLG78_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21868.1"
                     /translation="MKHILFIFAFLSSCASARYIGTQPGEPGSRLTVIAGDVDISFVT
                     FDNVSVSDSTRTIYTEPGSHKASFRYQSRRLYSVQNAEIEFSGNRGDTVMACYTSVIR
                     SGSYPNFDWRWGIFPLVYPGQVDATSLFVKGENPACLLWSGIGRESIFMQENIDQGAR
                     RDFKNLKGQTLLQFAKSIGAPAENIARLEKNGFN"
     gene            complement(3843..4214)
                     /locus_tag="PLG78_01810"
     CDS             complement(3843..4214)
                     /locus_tag="PLG78_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21869.1"
                     /translation="MTGVAAENAIVVKTHRFSKKEEIPQEPAPAPEVEGIVMNQLESI
                     TVDEGKLLKETFPNLDTRKTVFIAENKMPFMHDSIWVALFCAAVSVGAILLHLSGKKS
                     LAKFEQNQKMKNMGSGPVATG"
     gene            complement(4298..5059)
                     /locus_tag="PLG78_01815"
     CDS             complement(4298..5059)
                     /locus_tag="PLG78_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21870.1"
                     /translation="MLRRMLCQNCGIEAPTREVTYYQNIGALVMRFHKKFQGRVCKRC
                     LHKLFFEYAGISAVAGWWGVISMIVNPFMILIDIFQYLTRLTMPPVPANAKFPQLTQM
                     EIDRLEPETTAIFQRLNTGANLETVVTEIARRENVTPGQVILFLRQKLNQQAQGSAAP
                     APVLPKGPTRPSVLPLPVAAGIVIFCGVIAYAGWNQKALEMSFEPKIAKLSDLAGKSS
                     FENRHVKITDGLALYPLSVFPPGRGHSRGKPQRKV"
     gene            5235..5912
                     /locus_tag="PLG78_01820"
     CDS             5235..5912
                     /locus_tag="PLG78_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE21871.1"
                     /translation="MPHSALLLESLSPDLLVRIIEEAFIVSITDAKGLIRYVNDTFCK
                     VSQYDRSELIGKPHRIVKSDVHSRDFFKSMWDTIGRGESWHGEICNRKKSGDLYWVDS
                     FILPIAGDTGAPVGYLSIRYEVTQLKEHEQTVQSQESLLIKKNRRLNNLAFWIAHDIR
                     PPIANLIGLSEGMTDALKGGADGSQFAQRVSEEAIRADQILRKIMRTAIASDIVDEQD
                     SEWLSGK"
     gene            5894..6247
                     /locus_tag="PLG78_01825"
     CDS             5894..6247
                     /locus_tag="PLG78_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE21872.1"
                     /translation="MVVWQIDDDPVHTELVEMIIRRTGFSNDTRSFHSARAALKALDQ
                     SLPDLILLDLRMPDMDGWEFIELTRSRKMPPIVIVTSSIDAREKKRALEIPTVRGWIE
                     KPVRVEDIVDLAVDD"
     gene            complement(6250..6657)
                     /locus_tag="PLG78_01830"
     CDS             complement(6250..6657)
                     /locus_tag="PLG78_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21873.1"
                     /translation="MKRFYGIPAFVVMMAIFAGGLSAETRTYRSSQVIGKFVPRMVDE
                     LGNAGFTFTVKTAYNEKRDGKQGFVFLLQPRNTVCELVFFDEAGKSSLVRIRTQDGAD
                     TDRFHKFFTQRLSMTEVGVTSAPQPAPSGWPTP"
     gene            6722..7075
                     /locus_tag="PLG78_01835"
     CDS             6722..7075
                     /locus_tag="PLG78_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21874.1"
                     /translation="MSGPAPFSIVKKPNDLLFFDNLALYYVLQNTPPRVLARVFLKAD
                     SRLSGSLLGILSPKQRETVHFMMTQENDDDAAKNLDAQNGLCLISEGLIARGLIKKQG
                     AHFFGVPGHEKVEPG"
     gene            complement(7123..8229)
                     /locus_tag="PLG78_01840"
     CDS             complement(7123..8229)
                     /locus_tag="PLG78_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNE21875.1"
                     /translation="MSAPGRICLFGEHQDYLGLPVIAMAVNLRFEIQFEFTDSKTIRI
                     SKPDLGPDVVTDFDPYARQPANSEDFCWGIAQVLIEDGFTFPRGGKATFRSRIPLRAG
                     CSSSSAMSAAWMRLLLEIGEHPEKSKYIDHPETSAYAVYRGEKEKFQGAGGMMDQYSC
                     YLGGLIHVFPDSSRPIPYGVEQLPAKLDGIILIDSGDPKDTQGILSSVGDRARKSIEI
                     AANEMPGFNLLTSNTAEVEGSIPAEKREKTEWKIAVDHLKNRDLCREGLRMFRNTIDP
                     ESLGSMLTEEHRILSGTLGISTPRIDWILDLANKAGAAGGKINGSGGGGTCFCYVPDQ
                     KARMQVLYTLAENNIRHFEVHSDSGARMDQARTF"
     gene            8324..8743
                     /locus_tag="PLG78_01845"
     CDS             8324..8743
                     /locus_tag="PLG78_01845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNE21876.1"
                     /translation="MSGKKYILYCDGACRGNPGPASIGAVIYLEGVDEPVLEISKTIG
                     TATNNQAEYRSLIEGLKGLQRYEGHHEVRMDSELVVRQILGQYKVKNEGLKPLFLEAR
                     EIFQGMKCTIKHVPREQNREADRLANEALDNSSHRTN"
     gene            8871..9872
                     /locus_tag="PLG78_01850"
     CDS             8871..9872
                     /locus_tag="PLG78_01850"
                     /inference="COORDINATES: protein motif:HMM:NF022831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL1"
                     /protein_id="HNE21877.1"
                     /translation="MKHAKILAFALAALFATAIQAEGSYVGFGLGIQFNLASLGNTIV
                     NDGLQGTAAGPPPNVRANNGAGGPNCMLTQTCPADAFATDNQKVVVDEKTLVALSRLT
                     LGNMDAKVSGPMIGGELKAFYEKEFASGAWVRGGAGLTRKIAGGSTISHAAGIEWYNV
                     QWNYSRLNIPIMGGFKAGIGETVSVYAGGGLSYNKGFFQLTGTNLGNVPSTFLARYGT
                     SVGLVTTVGAGNQFNINPVTGAVTTPTSTSASLQGIWGSKAKFKAEGLGFNFVTGIEK
                     KLETGDKLFFEIGQVVAGGIGRRGFKDPGAISAFAPVAAYPVDLSGTTYSFGYKMHM"
     gene            10080..10838
                     /locus_tag="PLG78_01855"
     CDS             10080..10838
                     /locus_tag="PLG78_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE21878.1"
                     /translation="MKRLFSLILLVAVHSLSAQTLHMLIVADTADASIGSSTLEDLKH
                     MQNKAREIAANSGMKLNMTVLKAPEFNGTTTLAAVKKLDPGPNDTVFFYYSGHGFRTH
                     NSKTKWPNLYVKKPEPGLEYAKILEILDKKNPRLVIALGDMCNSFSDLKSSSGTDRAF
                     SEPQPENYRKLFVKTRGRIYASGSKPGQFSFAMNSGGAFTNQWIRALTTELKADNPAW
                     KRLMTAATKPIKIGSAGQATQDPIYELVEESAPQ"
     gene            complement(10888..12498)
                     /locus_tag="PLG78_01860"
     CDS             complement(10888..12498)
                     /locus_tag="PLG78_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE21879.1"
                     /translation="MSRNHWWKTTTIYQVYPLSFQDSNGDGIGDLRGIIHRLDYIASL
                     GFETIWLSPFYISPFRDHGYDVSSYTEVDERMGTDADIQELIDRAHTWKLRVILDMVM
                     NHTSDQHPWFVESKSSLDSPKRNWYIWKKGKGNQPPNNWISMTGKPGWFRDRKTDEWY
                     YANFLEFQPDLNYRNPAVKTEMFEMAKGWLRRGADGFRLDIFNSIYKDDQFRDNPFSL
                     VYFPRPHALDECFFQRKKYNLNLPESVDFARDLRNATDSTGDKLLLGEVSGDDRAIRS
                     FLGNGEDALGLVFNFAAIEYQYNAHFFADILKRNEEHFSDPYLPTLVFGNHDVPRSIG
                     RVHGSIEKWKVLAMLQFSARGVPVSYYGEEIGMTNGDVAVADAQDPIAQANKLIPKFL
                     AHLLGVFLTRDDCRTPMQWDSSPNHGFSTAKPWNPIGKSPERTVAGQERDEHSLLTFY
                     RKLLAVRKSHAALHSGVAEEIKTAGSILSFDRVYGKEKASVYLNFSSISARVKGGAGT
                     VLIATGEVLQHKDEIELGPHSALILAHD"
     gene            complement(12568..13515)
                     /locus_tag="PLG78_01865"
     CDS             complement(12568..13515)
                     /locus_tag="PLG78_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE21880.1"
                     /translation="MRIKLWGVRGSLPTPMGVQEYQTILGHALAHAQNDFRANPSITA
                     EQLYEGIPAQYRRVIGGDTTCVEVDYRGTCIVMDMGTGARRAGLSIAKRALRDIYVLI
                     THTHWDHIQGFPFFAPAYDPGVTIHFYSCIPDLHERFIRQQHPHYHHVKFEDLPCKTT
                     FTYVEQGEQFEIGNLQMEAQPLIHPGGSASYKIRKENQTFVFATDTEFYGPDLSMLVK
                     KYDQFFHGADLLIIDAQYSLTEAEQKRGWGHTAMTIAVDCAMHWDIERLGLTHHEPAH
                     SDETIRELFARAHEYLEANNPHRHMMELTLAREGEEYFL"
     gene            complement(13578..13949)
                     /locus_tag="PLG78_01870"
     CDS             complement(13578..13949)
                     /locus_tag="PLG78_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21881.1"
                     /translation="MGEVKASLPSDVFLVQKALGIEMEKRAAILSADGRELQRKTRES
                     EEVLRQMGQMAASPKPLSREAIRLAEKLREESGENQRLLEKANHTIHRLLTEMGEKES
                     TYDPHARKQQSTGGVLLDASV"
BASE COUNT         3300 a         3473 c         3697 g         3697 t
ORIGIN      
        1 atacagatgg gcttacggaa gcgcgcgaca gaacggggaa gctcatctgt tctgaagagg
       61 atctggcaga gcgagcgggg gatcgttcca gggatgtttc tactctgtgc gaagatattt
      121 ttcaagacgt gctttcaaaa actggaggaa tggatttcct cgaagacgat attaccttcc
      181 tcatcgctga gtatactggt agttgagctt tcgcttaata actagttagg tgcagaggcg
      241 ctatagtcgc agctgctaac gtttttagct tttgcgcacg gcaaagtacc ttgttttacg
      301 tcttgaagtc gcgcccaatc cgtggcgggg cagtggcatt gcttagccac aatttgatcg
      361 cagccggtac ccacgcgatg tttcagattg tcctcgccgg tcagcacaca gtgcaggacg
      421 cggatgggga gcacccgctg aaaatcttcg acggaaacga gtctatcatg gaggtgagcg
      481 ggcctttcga gttgcgcggt ggtcgcctaa cggaactatt tcttgaattt gatccgaata
      541 cttccgtatt ctttaccagc gagggttatg ttctagatgc agacattcgc gttgcatccg
      601 tactatcaat gacgcctgag caggagaata gaatagtgca ggcactcggt ccgaaaacga
      661 acctggttgc tgactccgcg gagcttgcat tggagggcca ggtggaaaca accacagttg
      721 ctgcggaaag gattccgaga acgcgcgtgt ccattcgcat tgaggaccgc cttcgcggac
      781 tcccagcaaa catgaatgcc cttcagctca cggttccagg cggtgtcgtg aatggtttgc
      841 ggctgcgcgt gtatggcatg ccgcagtttt cccagaacga gcgagtgatt ttgttcttga
      901 agagaattgg aaatgaatac ggaattgtgg aaggcgaata cgggaaggtg agcctatgaa
      961 acgagcgcga tggctgttct ttctgccttg tctagtttct cttaattttg tgcccgtaga
     1021 gcagggattc aaattccctg gagactggcc aaagatccca ttctatgtga atccatctgt
     1081 gccaggagaa aggattggcg gtacggatgc gctcatgaac gcaatgagag atgcttcagc
     1141 gatgtggtcg tcggacgtga ccaatgttcg ttttgatttc agagtttccg ggttttcaga
     1201 tcgcgctcaa ccttttgcgc ctgcggtgct ggattgtagc tcccaaacga ctgcggattt
     1261 tgcgcagact gccaagacag tatttgcttt ttatgcgtgc ggatttgaag agtatgattt
     1321 tgattcgaaa ggcaatcata gatgaacaga atcgtcgcta gtattttggc gttattactg
     1381 agttcgtgca ccgcgatctt cggaccgagg gatagctgtg acgctttctg caaagaggca
     1441 caagcatttt cccagttgct tgtattgatt ccaatcctgc gctgcgcaca tccactcggg
     1501 tataaagtgc gtttttcaag cggcgctgtg gactgcatca ttccctctac atctccctat
     1561 atctacaatc aaagcactac ggcatatgcc ggatccacag tgactggcgt ggttactctg
     1621 gagtgtcgcg caccaactgc catcaaggtc tggagcaaga tcgcgacttc gaaccccgcg
     1681 ccgggatcta cggctacatt cgttgtgtct ggaaattcaa cgctcgcgag ttactcggtg
     1741 ggcgacacga tcttctgctc cgacgaaaac cttttctatc acaaggtaac cattctatga
     1801 atccaattcg tcaggtttct tatctgggtt gcgcgtcgct ctgtccctct ggtcaaatgc
     1861 gctatttccg caggacccgg gcgggattgg acaggcacca tcatgcgcgg actatccctt
     1921 tggacgcaac ataactgtta ccgggcacag ccgcgcctac gaggttgcgg tgttcacttt
     1981 gccagccgac tatggaagtg ccattgggga cgcaaggctc cgcgatgacg ccactgacgc
     2041 gaccaatgtg aagatttttg ctccgggagc agtaaccggc gttcagaatc ttgcctggcc
     2101 cggagcgcag gcgacggatt cagcgccagc acattgggtc atgggcttga tcatatctat
     2161 ctttgattcg tatcttccta aagataactt ctggagcgtt gtggttcatt tgcccgaatt
     2221 cttgagaaac ccgatggttc atctaaattt gcaacaaaca gttttggtgt gggggatatc
     2281 ctgttcaatg ccgcatccgg gaacaactgg caaaggggaa tcgcgcgctg ctacacaaac
     2341 ggttctccat atcaaggttc agataacatc gtattgaatg tcggcggtaa cgatttgttg
     2401 aatttctaca tgcgcaagca tacgtaccaa gcggagtacc ttttcgtgcg ttacgtcttg
     2461 aatccgaagg gctggctcaa gcttattttc gatcgtggcg ctgtagacag agagcttgag
     2521 attttttgga tgtggagcat gcacctgaac aatttcagaa tagcactcgc cgtcgaatcc
     2581 atagggaatt acatctgagc gactttcacg gacccggctg ggatcatgtg gggcgctatt
     2641 ctaagcttct gctggttgag gaagcgcctt ccctggcttc atgggtggtc aatttgtttc
     2701 ccgtccctgg cggcggagcg tcagacgatc caccgtggcg tatcatttta tattttgcgg
     2761 acccgtagcc atcccggcat ttgtgaacgc gaggaaacgg aagaatttcg gctgcgcgac
     2821 agtcagttgc ggcttttgtt gaatgatcgt cagaaagaag actgtcactg agggcggatc
     2881 gaagctcagt cagggtgcgc gcgaccacct gtaagtctgc ccgcccgaaa cgcgctgcaa
     2941 acaagctacg tcgtgcgctg cgtcccggcg gaagaatcgt aacgaactac ctggcccgcc
     3001 caccgcggcg caacttcgga tgactacgga gtatttgcag agcaatttgc ggcattggga
     3061 cgcgctgcag gcttccagaa tgtgcaagtg tacactctgc ccatgcgtcc aatcccctgt
     3121 tgcacaattg tttttgaata gcggctgcct tgcaacggag ttgcgcccct gtcgcctgat
     3181 cgctcccagg gatgagcttt cgcttaacaa ttagttaaag ccatttttct caaggcgggc
     3241 aatgttttcc gcaggtgcgc cgatgctttt tgcaaactga agtagtgttt gccctttcaa
     3301 attcttgaaa tcccggcggg ctccctggtc gatgttctcc tgcataaaga tgctttcgcg
     3361 cccgattcca ctccagagga gacaggctgg attctctccc ttaacaaata ggcttgttgc
     3421 gtcaacctga cccggatata caagcgggaa gattccccag cgccagtcaa aattaggata
     3481 gctgcccgac ctgatgacgg aggtatagca cgccatcaca gtgtcgccgc gatttccgga
     3541 gaattctatt tctgcatttt gaaccgaata caagcgacga ctctggtagc gaaagctagc
     3601 cttgtggctc cctggctcag tgtagattgt tctcgtggaa tcgctcaccg atacgttgtc
     3661 gaaggtgaca aatgaaatgt caacgtctcc ggcgatgacg gttagcctgg aacctggttc
     3721 tccgggctgg gttccaatat accgagcgga agcgcacgag ctcagaaagg cgaaaataaa
     3781 caaaatatgt ttcacatgtt cctctgatat ggcgcatttg cgcaatgccg cataacgcta
     3841 cactagcccg tcgccaccgg cccggaaccc atgttcttca ttttctggtt ttgctcgaac
     3901 tttgccaggc tcttttttcc gcttagatgt agaaggatcg caccaacgga aacggcagcg
     3961 cagaacaacg cgacccatat ggagtcgtgc atgaatggca ttttgttttc cgcaatgaag
     4021 actgttttgc gtgtgtccag gttcgggaaa gtttctttga gcaacttccc ttcatccaca
     4081 gtgatcgatt cgagttgatt catcacaatc ccttccacct caggagcagg ggccggttct
     4141 tgcgggattt cttccttttt tgagaagcga tgcgtcttga cgacgattgc attttcagca
     4201 gccactccgg tcatggctgg cccggccgct gtctgggtga tttgaaagtt ctggagaacg
     4261 gatctgtcat tggggaccag taagtagtac gtataattca gaccttcctc tgtggttttc
     4321 ccctgctgtg tcctcttcca ggtggaaaga cggaaagtgg atagagagca aggccatccg
     4381 tgatcttgac gtggcggttt tcaaaactgc tcttgcctgc caggtctgag agctttgcga
     4441 tctttggttc aaagctcatt tcgagagctt tttgattcca accggcatag gcgatgacac
     4501 cgcaaaagat tacaattcct gccgcaactg gaagtggcaa tacagaaggg cgcgtaggtc
     4561 ctttgggaag aacgggtgcc ggggctgcgc taccctgggc ttgttggttt agtttctgtc
     4621 tgagaaatag aatcacctga ccgggcgtta cattctctct gcgcgcaatc tctgtcacca
     4681 ctgtctcaag gtttgctccg gtattcagtc tttgaaagat ggccgttgtt tctggttcga
     4741 gtctgtctat ttccatttga gtgagctgcg gaaatttcgc gttcgcagga accggaggca
     4801 tggtgaggcg agtcagatac tgaaagatgt caatgaggat catgaatgga tttacgatca
     4861 tggagatcac accccaccag ccggcgactg ccgatatccc ggcgtattcg aaaaagagtt
     4921 tgtgcaaaca acgtttgcaa actcttccct gaaacttctt atgaaagcgc attacgagtg
     4981 cgccaatgtt ctggtagtac gttacttccc ttgtgggggc ttcaattccg caattctggc
     5041 atagcatgcg ccgaagcaca gagtacgaaa ctcattcgta aatcactttt tgcaaaagca
     5101 ctttttctgt ctcatggggc gggtgatttt gagacgatct gtttgctttg tgccgttcgt
     5161 taccagaaat gtgacgttca ttcacccccg gaatattttg tttgcggatt ctaaaccaga
     5221 ggaaattttg cgccgtgccc cactcagcgc tattacttga gtccctttcg ccagaccttc
     5281 tggtgcgaat cattgaggag gcgttcattg tttccatcac agatgcgaag ggactgattc
     5341 gatacgtaaa tgatacattt tgtaaagtat ctcaatatga cagatctgaa ctgatcggga
     5401 agccgcacag aatcgtgaag tcagatgttc attcgcggga cttcttcaaa tccatgtggg
     5461 acacgattgg tcgcggagaa tcctggcatg gagagatctg caatcgcaag aaaagcggag
     5521 atttatactg ggtggatagt ttcattctgc ccatcgccgg agacacagga gctcccgttg
     5581 gatacctctc gattcgatat gaagtcacgc agctaaaaga gcatgagcag acagtccaaa
     5641 gtcaggaatc tctgttgatc aagaaaaaca gacgattgaa taaccttgca ttctggatcg
     5701 ctcacgacat tcggccgccc atcgcgaact tgattggtct ttcggagggc atgacagatg
     5761 ctttgaaggg cggcgcagat ggatcgcaat ttgcgcagcg cgttagcgag gaggcaatac
     5821 gagcggatca aatcctcagg aagatcatgc gcacggccat cgcaagtgac attgtggatg
     5881 agcaggattc ggaatggttg tctggcaaat agacgacgat ccggttcaca ctgagcttgt
     5941 tgaaatgatc atccggcgaa ccggattttc aaatgacact cggtcctttc attcagccag
     6001 ggcggcgctc aaagcccttg atcagtcttt gccggatctg atcctcctcg acttgcgaat
     6061 gccggatatg gacggctggg agttcattga actcaccaga tctcgcaaga tgccgcctat
     6121 cgttattgtt acttcttcta ttgatgcgcg agagaaaaaa cgcgcgctgg aaataccgac
     6181 cgtgcgcgga tggattgaga aaccagtccg cgtagaagac attgtagatc tggcagtgga
     6241 cgactgatct cagggcgtag gccagccaga aggtgctggc tgcggagcgg atgtaactcc
     6301 cacttcagtc atgctaagcc gttgggtaaa aaacttatga aagcgatctg tatccgcgcc
     6361 gtcttgtgtg cgaatgcgaa ccagagatga ctttccggct tcatcaaaga agaccagctc
     6421 gcagactgtg ttgcgtggtt gcagtagaaa aacaaagccc tgcttgccgt ccctcttttc
     6481 attgtatgct gttttcaccg tgaaagtgaa tcctgcattg ccaagttcgt ccaccatgcg
     6541 cggaacaaat ttgccaatca cctgcgagga ccgatacgtg cgcgtttctg ctgacaggcc
     6601 gcctgcaaag atcgccatca ttaccacaaa tgctggaatt ccgtagaaac gtttcatctc
     6661 actctccagt tcggcaaaaa aagcattttc ttaaccgttg tccgtgctaa atcggctaaa
     6721 tatgtcagga ccggcgccct tttctatagt aaagaagcca aacgacctct tgttctttga
     6781 caatcttgcg ttgtactatg ttctacagaa tactccacca agagttctgg cccgcgtttt
     6841 tctcaaagcg gattcgaggc tttcgggaag tctgcttggc attcttagcc caaaacagcg
     6901 cgaaactgta cacttcatga tgactcagga aaatgatgac gacgcagcca agaatctgga
     6961 tgcgcagaat ggtctttgtt tgatttcaga aggtctcatc gcgcgcgggt tgatcaagaa
     7021 acagggcgcg cacttctttg gagtgcccgg tcacgaaaaa gtagagccgg gctgaccgag
     7081 cgagtgccag atcgtgactg ctacttttct ctttcgcctc ttctagaacg ttcttgcctg
     7141 atccatgcgt gcgcctgaat cactgtgcac ttcaaaatgc cggatgttgt tttcagcaag
     7201 tgtgtagaga acttgcatgc gcgccttctg atccggaaca tagcagaaac aggttccgcc
     7261 tccacctgaa ccgttgattt ttccgcctgc agcccctgct ttgtttgcaa gatccagaat
     7321 ccaatctatg cgcggcgtgg atattcctag agtcccggac agaattcggt gttcctccgt
     7381 gagcatcgag cccaggcttt ccggatcaat tgtatttcta aacatccgca gtccttctct
     7441 acacagatcg cgattcttga ggtgatcgac tgcaatcttc cattcagttt tctcgcgctt
     7501 ttctgcgggg atggatcctt caacttctgc cgtgttcgaa gtcagcagat tgaatccggg
     7561 catttcattg gcggcaattt cgattgattt tcgcgcgcgg tctccgacgg agctgagaat
     7621 tccttgcgta tctttaggat cgcctgagtc gatcagaatg attccgtcga gctttgccgg
     7681 caattgttcc acgccgtaag ggatgggtcg agacgagtcc gggaagacgt ggatgaggcc
     7741 gcccaggtag caggagtatt gatccatcat tccacctgcg ccctggaact tttctttctc
     7801 tccgcgatag actgcatagg ctgatgtttc cggatgatcg atgtatttgc ttttttccgg
     7861 atgttcgccg atttccagta acaatcgcat ccatgcggca gacatcgcgg aagaagaaga
     7921 acagcccgcg cgcagtggaa tgcgcgaacg aaacgttgcc ttacctccgc gcggaaatgt
     7981 aaagccatct tcaatcagaa cctgtgcgat tccccagcag aagtcttccg agtttgccgg
     8041 ttgcctggca taaggatcaa agtccgtgac cacgtcgggt ccaagatctg gttttgatat
     8101 tcgtatggtt ttggaatcag tgaattcaaa ctgaatctca aagcgaaggt taaccgccat
     8161 ggcaatgacg ggcagtccca gataatcctg atgttctccg aatagacaga tacgcccagg
     8221 cgcgctcaca aacagcatgc ctcgtgatgc aggaacgctt gaaacggaca attctaattc
     8281 tattgcttga cgcgctatcg cacaggagtc gtatcttcag gtcttgtccg gtaagaaata
     8341 tattctctat tgcgacggag cttgcagggg caatccaggg cctgcgtcca ttggcgccgt
     8401 catctacctg gaaggggtgg acgagcctgt gcttgagatt tccaaaacaa tcggcacggc
     8461 cacaaacaac caggcagaat accgttcatt gatcgagggt ttgaagggct tgcagagata
     8521 cgaaggccac catgaggtcc gcatggactc agagctggtg gttcgccaga ttcttgggca
     8581 atacaaagtc aaaaatgagg gccttaagcc tctttttctg gaagcccgcg agatttttca
     8641 ggggatgaag tgcaccatca agcatgtgcc ccgggagcaa aaccgggaag cggatcgtct
     8701 ggcaaacgag gcgctggaca actcctcgca taggacgaat tagaccgatt ctgcccggcc
     8761 ttcgttccct ttttttttat ttgacaacca cctttttggc cgctttttca agtctgcgct
     8821 ggcggagggg gaatgcactt gtccgtcgtg caaaaattta ggagtaaaag atgaaacatg
     8881 cgaagatact tgccttcgcg ctggcggccc tctttgcgac ggcgatccag gcagaaggca
     8941 gttacgttgg ctttggtctg gggattcaat tcaacctggc cagcctcggg aacactattg
     9001 taaatgacgg tctgcaaggt actgctgctg gcccaccacc aaacgttcgc gcgaacaacg
     9061 gtgcgggcgg cccaaactgt atgctcactc aaacttgtcc tgcagatgca tttgcaactg
     9121 acaaccagaa ggtagtcgtc gacgaaaaga ctctcgtagc tctttccaga cttactcttg
     9181 gtaacatgga cgcgaaagtt agcggtccaa tgattggtgg ggaactcaaa gctttctatg
     9241 agaaagaatt tgcaagcggc gcgtgggtgc gcggtggagc aggtctcact cgcaagatcg
     9301 cgggtggtag cacaatttct cacgcagctg gtattgaatg gtacaacgtt cagtggaact
     9361 acagccgttt gaacatccct atcatgggcg gtttcaaagc cggaatcggc gaaactgtat
     9421 ctgtatacgc tggcggcggt ctgagctaca acaaaggctt cttccaattg actggaacaa
     9481 acctcggtaa cgtaccaagt acgttcctcg ctcgttacgg aacttctgtt ggtctggtaa
     9541 ctactgtggg cgcgggcaac cagttcaaca tcaaccctgt tacaggtgca gtgactactc
     9601 ctacaagtac atctgcgagc ctccagggta tctggggatc aaaagcgaaa tttaaggcag
     9661 aaggccttgg atttaacttt gtaactggta ttgaaaagaa actggaaact ggcgacaaac
     9721 tgttcttcga aattggacag gtagttgctg gcggcatcgg tcgtcgcggc ttcaaagatc
     9781 caggtgcgat cagcgcattt gctccagttg cagcttaccc tgttgacctt tccggaacaa
     9841 cctattcatt cggatacaaa atgcacatgt aattgtgttt aaactttggg gaaaagccgc
     9901 gctctcgagt gcggcttttt ttttgcgcga tatttgaaac tgtccggttc atttgacccg
     9961 ggtgaaaaat gcatcattgt cgctttacgc gaaaatgatc tgatcaggtt cgcaagatga
    10021 acctttgagt tcttctttac gcctaagaat aaaggtgctg aaatgtttgc ggggtgttta
    10081 tgaagcgttt attctctctg attcttctgg tcgcggtgca cagcctgtcc gctcaaactc
    10141 tgcatatgct catcgtggca gacactgcgg atgcgagcat cgggagttcc actctcgaag
    10201 atctaaagca catgcaaaac aaggcgcgcg agatcgctgc gaattctggc atgaagctca
    10261 acatgaccgt tttaaaagca cccgaattta atggtacaac cactctggcc gctgtgaaga
    10321 agctcgatcc aggcccaaat gataccgtct tcttctatta ctccggccat ggatttcgca
    10381 cgcacaattc gaaaaccaaa tggcccaact tgtatgtaaa gaagccagaa ccgggtctcg
    10441 agtatgcgaa gatcctggaa atcctggata agaaaaatcc gcgtctggtc atcgcacttg
    10501 gagatatgtg caatagtttc tcagatctca aaagcagtag tggaacagat cgcgctttct
    10561 ctgagcccca gccagagaat tacaggaagc tctttgtaaa gacgcgcggc cgcatctatg
    10621 cttcaggatc gaagcccggt cagttttctt tcgccatgaa tagcggtgga gcttttacga
    10681 atcagtggat cagagcactg accacggagc tcaaggcaga caatccagcc tggaagaggc
    10741 tgatgacagc ggctacaaag cccatcaaga ttggaagtgc tggacaggca acacaggatc
    10801 cgatctacga actggtagaa gagtccgctc cacaataggc ggcttcacag gttgttagat
    10861 gagccccgtc gtctggactc gcgtaattca atcgtgtgca aggatcaaag cagagtgtgg
    10921 cccgagttca atctcatctt tgtgttgcag cacttccccc gttgcgatca gaacagttcc
    10981 ggcaccacct ttcacgcgag cggatattga gctgaagttg agatagacgc tggccttttc
    11041 ttttccatag acacgatcaa aactcaggat ggacccggct gttttgattt cttccgccac
    11101 acctgaatgc aatgcagcgt gcgatttgcg cactgccagg agttttctat agaacgtaag
    11161 gagagaatgc tcgtctcttt cctgaccggc caccgttctt tccggtgact ttccgattgg
    11221 attccacggc tttgcggtcg agaaaccatg gttaggtgaa ctatcccatt gcatgggcgt
    11281 gcgacaatcg tctcgcgtga ggaatactcc cagcaggtgt gccaggaatt ttggaataag
    11341 tttgtttgct tgagcgattg gatcttgcgc atccgcaacg gccacgtccc cgtttgtcat
    11401 gccaatctcc tctccgtagt aggagacggg cactccacgt gcgctgaatt gcagcatggc
    11461 cagaaccttc cacttttcaa tagatccgtg aacgcgtcca atggagcgag gcacatcgtg
    11521 gtttccaaaa acaagagtgg ggagatatgg atcgctaaaa tgctcttcgt ttcttttgag
    11581 gatgtccgca aagaagtgag cgttgtattg atactcgatg gccgcgaaat tgaaaaccag
    11641 accgagtgcg tcttctccat tgcccagaaa gctgcgaatg gcgcgatcat cgccgcttac
    11701 ttcacccaga agaagtttgt ccccggttga atctgttgcg ttgcgcagat cgcgcgcgaa
    11761 gtcgacgctt tcgggaagat tgaggttgta cttcttcctc tggaagaagc attcatcgag
    11821 cgcgtgcggt cttggaaagt agacgaggct aaacggattg tctctgaact gatcgtcctt
    11881 gtaaatgctg ttgaatatgt ccaggcgaaa tccgtctgct cctcgtctca gccatccttt
    11941 ggccatttcg aacatttcag ttttgacggc ggggtttcta tagttcagat caggctgaaa
    12001 ttctagaaag tttgcgtagt accattcatc cgtctttcga tcgcggaacc atcctggctt
    12061 gccagtcatc gaaatccagt tgttgggagg ttgattccct tttcccttct tccagatata
    12121 ccagtttctt tttggagaat ccagtgagct tttggattct acaaaccatg gatgctggtc
    12181 cgaagtgtgg ttcatgacca tgtcgaggat aacgcgcagc ttccacgtat gggctctatc
    12241 aatgagctct tgaatgtccg catctgttcc cattctttcg tctacttcgg tgtaggagct
    12301 cacgtcatag ccatgatcgc gaaacgggct tatgtaaaac ggagacagcc agatcgtttc
    12361 aaagcccagg gaagcaatgt agtcgagccg gtggatgatg ccgcgaagat cgccgattcc
    12421 atctccattg gaatcttgaa acgaaagagg atagacctga tagatggtcg tggttttcca
    12481 ccagtggttg cggctcatgt ccgagtctgg tcactggcag cgcgcaattc aatcaattat
    12541 tgcaaatcag gcgctgtccc gggcagttta taggaaatac tcttcgcctt ctcgcgccag
    12601 agtgagttcc atcatgtgcc tgtgcggatt gtttgcttcc agatattcat gggccctggc
    12661 gaacagctct cgaatggttt catctgagtg cgccggttca tgatgggtga ggcccagtcg
    12721 ttctatatcc cagtgcatgg cgcagtccac tgcgatggtc atggctgtgt ggccccatcc
    12781 tcttttttgt tctgcttccg tcagggaata ctgcgcgtca atgatcagca agtcggcacc
    12841 gtgaaagaac tgatcgtatt ttttgacgag catggaaaga tctggaccgt agaattcggt
    12901 gtccgttgca aaaacgaacg tttgattttc cttacgtatc ttataagatg cagagccgcc
    12961 tggatgaatc aatggctgtg cttccatttg gagattgcca atctcaaact gctctccctg
    13021 ttctacgtag gtgaatgtag tcttgcatgg cagatcttcg aatttaacat gatggtaatg
    13081 cggatgctgc tgccgaatga atctttcatg aagatctgga atgcaagaat agaagtggat
    13141 ggtgactcca ggatcgtaag caggtgcgaa aaatggaaag ccctggatat gatcccagtg
    13201 tgtgtgagta atgagaacgt agatgtcgcg taacgcccgt ttggcgatgg ataagcctgc
    13261 ccgtcttgcg ccggttccca tatccataac aatgcacgtg ccgcgatagt ctacttccac
    13321 gcaggttgta tctccgccta tgacgcgacg gtattgagct ggaattcctt cgtagagttg
    13381 ctctgcggta atagatgggt ttgcgcgaaa atcattctgc gcatgggcga gagcatgacc
    13441 gaggatggtc tggtactcct gcacacccat gggtgtggga agggagccac gaacacccca
    13501 gagcttgatt ctcattcgct cttccactta aagacagaca acggttctga tttgaaaaat
    13561 ccaatcaaaa aaattgatca gacgctggca tccagtagca cgccgcccgt agactgttgc
    13621 ttgcgagcgt gcggatcgta tgtggactct ttttcgccca tttctgtcag gaggcgatgg
    13681 atcgtatgat ttgctttttc gaggagtctc tggttctctc cggattcttc tctcagtttt
    13741 tcagcgagtc gaatggcttc ccgggacaga ggcttaggcg aagctgccat ttgccccatc
    13801 tggcggagca cttcttcgga ttctcgggtc tttctctgca attctcgtcc atctgcagac
    13861 agaatggctg cccgtttttc catttcgatt cccagtgctt tctgaaccag aaagacatct
    13921 gaggggaggc ttgcttttac ttcgcccata ctctattcat cgactgggca tgacttccct
    13981 tgagctgatt ttttgttgac cgcagcccgg tttggcacgt cccattgagg aaagcggagg
    14041 ctttatgtct aaatccatgc gaatttccgg tcttataacg ctcctggcgg ccttgagcat
    14101 ttcattgttc gtatactgtt cctcagggtc ttcgaaaaaa gattctgacg atgataagac
    14161 tgtattc
//
LOCUS       DAOQJL010000023        14130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2083_length_14130_cov_4.602558, whole genome shotgun sequence.
ACCESSION   DAOQJL010000023 DAOQJL010000000
VERSION     DAOQJL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_14130_cov_4.602558"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLG78_01875"
     CDS             <1..386
                     /locus_tag="PLG78_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE21882.1"
                     /translation="IRVPLWTAFTNHWNILEKSGNLEAARLRLADARLKAKTDLQASL
                     DRMDGIKKGLRLHDQSLIPGAMNAHEAARISYANGKADFSIVIQSLQVHFDHELSRLE
                     HELEFTAELLKAAESLDAFFPGKDK"
     gene            383..1408
                     /locus_tag="PLG78_01880"
     CDS             383..1408
                     /locus_tag="PLG78_01880"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE21883.1"
                     /translation="MKTKWIVIFVFAAAVIVIIAARGKQLLTIFKYSQQAAVDPAQAA
                     ISYWTCSMHPQVHKDGPGLCPICHMQLVPVYKEDQSHQHDTGAGVVKVTEARQKQGGI
                     KLSVVKKEPFQVTLRTTGKAALDTELASAVKEFITLGKEDPALARSAAARLRILGMGP
                     EEIRQLELNPASVESLYQVATPGIAWVYATLYEQEVDLIKVGQTAIISPSYAPEKELT
                     GTVRSVSSVVDQASRSVRARILVRDPQGILRPEAYLRVTVQANLGPRIVIPRSAVVYT
                     GKRQIVFVAADADTFQAREIKVGQESGERIVIAAGLNEGDRIVTSGAFLIDSESRLQS
                     AIDEAGE"
     gene            1410..4655
                     /locus_tag="PLG78_01885"
     CDS             1410..4655
                     /locus_tag="PLG78_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNE21884.1"
                     /translation="MVERIIRFSAKNRFLVLLMTGVLLWGAYWCAKHIPLDALPDLSD
                     TQVIIYSRWERSPDIMEDQVTYPIITGLLGAPRVKAVRGFSDFGYSFVYVIFEDGTDL
                     YWARSRVLEYLARIQPRLPEGVRAEIGPDATGLGWVYQYALTGDGSVEQHEIRAFQDW
                     NLRLHLQTVPGVAEVASLGGPVKQYQVLLDPGALLSYNISVPTITDAIRKSNRETGGR
                     VVEFSGHEYMVRGRGYVRETRDLENIVVRVDDAGTPVLLRNLGKVTLGPDIKRGASDM
                     DGEGEVTTGTVVMRSGENALAVIERAKTRLSEIQSSFPEGVKLVTLYDRSELIEKAIE
                     TLKSTLVEEIIVVSLIILLFLWHLPSATTAIITIPVSVFLAFIPLYLTGQTSNIMSLA
                     GIAISIGVLVDGAIVQVENMYHRIQIWMESGQKEPFEEVRLRALTEVGSSVFFSLLVI
                     AVSFMPIFTLVDQEGRLFKPLAYSKNFAMAIAALLALTLDPAIRMMYNRYEFFRFRPV
                     WLATLMSHLLVGRYYKEENHPVSRVLFRFYEPACNFVLKHRKATLATAVLLMISTIPI
                     YGKLGSEFMPPLNEGAILYMPVSLPGISIGEAKRSLQIQDRMLREFPEVERVFGKVGR
                     ADSATDPAPLSMVETVIVLKPESQWREVKRWYSFLPEWSKEPFRRVWRDRITYEELVQ
                     EMDQKIRIPGWTNAWTMPIKNRIDMLSTGIRTPVGIKISGSDIKEIEKIGLELETIVK
                     EVPGTRSVYAERVAGGYFLDFNLKREELARYGLTVDDALTIVESAIGGENLSTTVEGR
                     RRFPINIRYAREFRETLPDLKRILVSTPSGAQIPISYIAEVRFNSGPSMIRDEDGLLS
                     GYVYIDIAGRDIGSYVEDAKKLVNSKLKLPQGTTVRFSGQYENMLRVRERLLVVVPLT
                     LFLILVLMYANTGSAVKTAIIVLAVPFSIVGAIWLLFLLGYNISIAVWVGMIALMGLD
                     AETGVFMLLFLDLSYKEWHDSGRLQTESDLRDAIVHGAVKRVRPKIMTVACGMIALIP
                     ILWSQGTGADVMKRIAAPMVGGLATSFVLELLIYPVLFYIWKIRTTELKVG"
     gene            4688..6199
                     /locus_tag="PLG78_01890"
     CDS             4688..6199
                     /locus_tag="PLG78_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HNE21885.1"
                     /translation="MQGQPNQLVLRKLGIDTQQEYIVFMRSDSSVCVSEGITSLMRVQ
                     ISCRGKTIVATVNAVTGNILGPGEASLSNRAFEILGASDGDTIRVSHLSALDSMSLVR
                     AKIYGRRLEQEHYDIIIQDIVHGYYSGVHLSAFIAGCVASKLDRGEVLGLTRAMLNAG
                     QRMTWPGIVADKHCVGGIPGNRTTPIVVSIVAENGILIPKTSSRAITSPAGTADTMEV
                     MAPVNLSLPEIRRVVEKFGGCIAWGGSAKLSPADDIFIRVERALDIDSESQMIASVLS
                     KKAAVGATHVVLDIPVGETAKVRSEAEAKRLSDLFEDIGAAIGLTVQTVITDGSQPVG
                     RGIGPALEARDVLCVLHNANDAPHDLKSKSILIAGQILEQCGKAIQGQGEEMALETLT
                     SGRALSRFMNICEAQGGFREPRKAQFQHDIVSDRSGRITSVNNRKIARLAKLVGAPAD
                     PCAGIEFLAPAGTRISRGDLLFTLHADSRGQLEYALDYYARQEDFLQMSEGAP"
     gene            6196..7080
                     /locus_tag="PLG78_01895"
     CDS             6196..7080
                     /locus_tag="PLG78_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE21886.1"
                     /translation="MILFCLGENQTLATSVCRAGQFDAGNLNSRKFPDGETYIRILDQ
                     VRNKHTAILCSLRDPDARIPSLLFLADALREQGAASVGLAAPYLCYMRQDRRFQPGEA
                     ITSRTFAKMISAGFDWLITIDPHLHRISSLSEIYSIPAVSLHAASLIADWVEKTVPEP
                     LIIGPDAESRQWAAEIARAHPCIVLEKQRLGDREVEIQGLENLPVGRTPVLVDDMISS
                     ARTMIVATELIRAQGFSPVCIAVHPLFHGSAFEDLKASGAATIASCNTITHPSNQIDV
                     SGMLASAIQSMLNEMQKS"
     gene            complement(7075..8424)
                     /gene="yegQ"
                     /locus_tag="PLG78_01900"
     CDS             complement(7075..8424)
                     /gene="yegQ"
                     /locus_tag="PLG78_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011078568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNE21887.1"
                     /translation="MKKPELLLPAGSLEKMRIAFDFGADAVYAGQPRYSLRARNNEFR
                     MEELAIGISEAHARGKKFYVTSNIIAHNNKIKSFLKDMAEVAAMKPDAVIMADPGLIY
                     LVRENWPELPIHLSVQASTTNYAAVKFWAKQGVERIILSRELSLKEVEEIKMQCPETE
                     IEVFVHGALCIAYSGRCLLSGYFNKRDPNQGVCTNACRWEYTLHPAAIDDSPRHPLGE
                     YEYLLTEATRPGEFMPIMEDEHGTYILNSRDLRAIQHVPDLMKMGVESLKIEGRTKSL
                     YYVARVTRAYRKAIDDMAAGLPFDESLIEDLEHLANRGYTPGFFGGTIPGDAHNDGAA
                     VSRSDKSRFCGMVASYDSNTKRGFIHVRNFMARGDEIEVLVPSGNHRFVISEMYDESG
                     NPLEVAPGNGWKIHVPMDADLTGGFVVRIASEDSRVSGETDAWKSPVQRDLTAVSAS"
     gene            complement(8458..10092)
                     /locus_tag="PLG78_01905"
     CDS             complement(8458..10092)
                     /locus_tag="PLG78_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE21888.1"
                     /translation="MKITYTNDCTVEETDSSLSLLQISLKHEIPHTHVCGGNARCSTC
                     RILVLKGAENLEPRNEAETALALRKGFTDDIRVACQTRAHGDVTVRRLVIDQDDIREI
                     VGTSIHTTGREMNLAILFSDIRSFTPFSENHLPYDVVHILNRYFNAMGEVILRNGGII
                     DKYIGDGLMAIFGLNSSDARTACLNAVRAGSSMLAELEHVNRYLQRTFQVNFNIGVGV
                     HYGEMIVGEMGHPKKVQFTALGDNVNMASRIESATKKAGVPFLISDAVYEHVKDQVTR
                     GKVFSAPLKGKTGRFKLYEIVEVAPGQGGTSEQYQLPLLQKMQIAQDTWAFLLDTSLN
                     DYAFEPGQFADLTLPEMESVPGQSTRTFSIASSPDRSDALMFVSRMRNSPFKNALMKL
                     PEGAPIFVSPAMGEMTIKNTPAPLAFIAGGIGITPFHSMVSWVLKEKPGTEVYLFYSI
                     RSLELAAFLDEFETYAAEFPNFHFIPTLTEADSPGWKYERGRIDKVMIRKYVPRDAYP
                     VFYVAGPSRMVTGMRELLVQMGVFDHDIHSEEFLGY"
     gene            complement(10095..10982)
                     /locus_tag="PLG78_01910"
     CDS             complement(10095..10982)
                     /locus_tag="PLG78_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21889.1"
                     /translation="MMRVCLTISIFVFLLSCRSAATRHELEYGGRAREYLVIPVEQPR
                     SMIFVLHGGGGKAAHMIRLAEGLPEIAKQRHILLVYPQGIDKGWNDGRDTKISTAISE
                     NVDDAGFLDSLALKLAADYKIPEDKIFITGISNGGMMSQRLACESRAFRAIASVAANL
                     QESFAPKCKPSAPISTLFILGTEDPLVPYGGGDISLFKKTRGRVLSADATLQFWRSHN
                     QCNADSVKTDIPDAVKDNTSATAETWSCGNARVRLIRVLGGGHTWPGGLQYLPEVVVG
                     RTSRDFSASEEIVRFFLGD"
     gene            complement(10989..13202)
                     /locus_tag="PLG78_01915"
     CDS             complement(10989..13202)
                     /locus_tag="PLG78_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNE21890.1"
                     /translation="MSTASRLLRYLEQNAGRKLPRKEVLSAFSSTGAFKAKAKSFRKS
                     RQVRGYQAESEVDVLLAELKLLNLVRTDGNKIIPASPFAAVAKASLSPSGAVFGAVRG
                     ADPASRDLFIPAAKSMNALTGDLVLVKLTDKTRDRFEGEVIEIREKARELYRMKLLSE
                     PVHRGIPGVLLDIHSRITACLATPRLPADLVSRMKQDVVVVVRMPGKTIMHMGLPLPE
                     AEFVRFESDTDMDPDYARVLMKHNLDPVYPEVEGLPEYDQEASEVKPENVFDWNSRID
                     LRDLYTVTIDGPDSKDFDDAISLKVESPRLWKLYVHIADVSHYVRIGTDLDREAARRA
                     TSVYLANRVVPMLPPVLSENFCSLIAERNRLAFTCEMDVRPDTGEILKYQIYKSVIRV
                     DKRLTYDLAETMLDESDSMLGRVWSLAKAQKAARLKAGRIDFDMREPKVIYDAEGRIK
                     EVLYRERLKSSMLIEECMLSANICTAEFQRKKEAHTLFRVHEPMDDMKLEALNHFLKI
                     YGINLELPNSDPENINHALKVVHEFTEKGKSVQKGSTRKLEGVPPDRIFQMLMLRSFM
                     QAKYMGEPLGHYGLGFRDYCHFTSPIRRYPDLVVHRALQNLIQKKKHAYTEAEISELG
                     FHTSEMERKAMDAERDIWKLKMLRSIERSGQKSFRGFITGFRPDRVFLELTDVSVEAI
                     VLHMHLTRDMELILPDPFSAYIKKLSRPAFLGEVWNLELERLDVEEMRLFVKPVW"
     gene            complement(13199..13861)
                     /gene="tsaB"
                     /locus_tag="PLG78_01920"
     CDS             complement(13199..13861)
                     /gene="tsaB"
                     /locus_tag="PLG78_01920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNE21891.1"
                     /translation="MNNYCLCIDTTHTHIVVGLFSKQNQYQIVRKAPREAFQNLFPAI
                     QEVLSAANVKRPSEIVTLTGPGSFTGIRICVSAARDLAQLWNVPVRGIGTLTCYSLPI
                     RNMEPGPFAVCIDGKQNRFYTKIIPESGLSATQMDLIPVQDLTAEEVAASGLPVYCDD
                     PDTFRARNGGDSRQLKEPEAAWIFAAHSEMPASGSYQELFPYYLRKDPAEARYPGGFN
                     IK"
     gene            complement(13836..>14130)
                     /locus_tag="PLG78_01925"
     CDS             complement(13836..>14130)
                     /locus_tag="PLG78_01925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNE21892.1"
                     /translation="TFNLHNRYEGSRGVLHHYDLYRLQKVAELEELGFLEDWHRVDHA
                     PTVHAIEWGDRALPHIGPGVPVFLVEISIDEKDDSLRHIQFYRRQGEQLLSLH"
BASE COUNT         3544 a         3662 c         3495 g         3429 t
ORIGIN      
        1 cgattcgcgt tccactctgg acagctttca caaatcactg gaatatcctt gaaaaatcag
       61 gaaacctgga ggcggcgcga ctcaggcttg ctgacgcgcg actcaaagcg aagacagatc
      121 tgcaggcttc gctcgacagg atggatggaa tcaagaaggg cttgcgcctc cacgatcagt
      181 ctctcattcc gggagcgatg aatgcgcatg aagccgcgcg catcagctac gcaaatggca
      241 aggcagattt ctctatcgta atccaatcgc tgcaagttca ttttgatcat gaactatcgc
      301 gtctcgagca cgagcttgag ttcactgccg aactactcaa ggccgcagaa agtcttgatg
      361 cgttcttccc aggaaaagac aaatgaaaac aaaatggatc gttatcttcg tattcgcagc
      421 tgcagtcatt gtaatcatcg cagcccgcgg aaagcagtta ctaacaattt ttaagtattc
      481 ccagcaggcg gccgtggatc cagcccaggc ggctatctcc tactggactt gctccatgca
      541 tccgcaagtg cataaggatg gtcctggtct ttgtccaatc tgtcacatgc aactcgtgcc
      601 agtttacaaa gaggaccaat cgcatcaaca cgatactggt gctggagtgg tcaaagttac
      661 ggaagccaga caaaagcaag gtgggatcaa actcagcgtt gtgaagaagg aacctttcca
      721 agtcactctt cgcacgactg gaaaagcagc gctcgatacg gaacttgcat ccgccgtcaa
      781 agaattcatc actcttggga aagaagatcc ggcgctcgct cgctctgcag cagcgcgcct
      841 gcgcattcta ggaatgggac cagaggaaat cagacaactg gagctgaatc ctgccagtgt
      901 ggaatcgctg tatcaggtgg ccactccggg catcgcctgg gtttatgcaa cgttatacga
      961 acaggaagta gacttgatca aagtaggaca aaccgcgatc atctctccct cgtatgcccc
     1021 ggagaaagaa ctcacaggta cggtgcgctc cgtgtcgagc gtggtagacc aggcatcgcg
     1081 tagtgttcgc gctcgcattt tggtgcgcga cccgcaaggg atactcagac cagaggcata
     1141 tcttcgcgtg acagttcagg caaatctggg tcctcgaatc gtgataccca ggagtgcagt
     1201 cgtatacaca gggaaaagac aaatcgtttt tgtcgcggcg gacgcagata ccttccaggc
     1261 cagagaaatc aaagtcggcc aggagtcagg agaacgaatc gttatcgcag ccggattgaa
     1321 tgaaggagat cgaatcgtaa cgtcaggcgc ttttctaatt gattccgaaa gcagacttca
     1381 atccgcgatc gacgaggcgg gtgagtaaga tggtagaaag aataatccga ttcagcgcaa
     1441 aaaatcgatt cctggttctc ctcatgactg gagtgctcct ctggggagcg tactggtgtg
     1501 cgaaacatat accgttagac gctcttccgg atctgagcga cactcaagtc attatctatt
     1561 ccaggtggga aagaagtcca gacatcatgg aagatcaagt tacctatccc atcatcactg
     1621 gactcctggg tgcgcccaga gtcaaagcgg ttcgcggctt ttctgatttt ggatactctt
     1681 ttgtttatgt gatcttcgaa gatggaacgg acctgtactg ggcccgaagt cgcgtgcttg
     1741 aatatctagc gcgaattcaa cctagattgc cagaaggagt cagggcggaa attggccccg
     1801 acgcaacggg cctgggctgg gtataccagt atgcccttac gggcgatggc tccgtagaac
     1861 aacacgaaat tcgcgcattc caggattgga acttgcgcct tcacttgcag acagtaccgg
     1921 gggtcgctga agtagcaagc cttggtggac cagttaagca ataccaggtt ctactcgatc
     1981 caggcgcgct gctctcgtat aatatcagtg ttcctaccat cacagacgcc atccgcaaaa
     2041 gcaatcgtga aacaggcggt cgcgtagtag aattttccgg ccacgagtac atggtgcgcg
     2101 ggcgcggata cgttcgcgaa acgcgggatc tggaaaacat cgtagtgagg gtcgatgacg
     2161 cgggaactcc cgttctactc agaaatctgg gcaaagtcac tctgggtccg gatatcaaac
     2221 gcggcgcatc cgatatggac ggcgaaggag aagtaaccac tggaaccgtt gttatgcgat
     2281 ccggtgaaaa cgcactcgct gtgatcgaac gcgcaaagac gcgtctgtca gaaattcaat
     2341 catcatttcc cgaaggagtg aagcttgtta ccttatacga tcgctccgaa ctgattgaaa
     2401 aagcgataga gactctgaag tccactctcg tggaagaaat catcgtagtg agtctcatca
     2461 ttctactatt cttgtggcat ctaccatccg ccacgactgc catcattacg ataccagtct
     2521 ctgtgtttct cgcttttatt ccgctctatt taacgggaca gacttcgaac atcatgtctc
     2581 ttgccggcat tgccatttcc attggtgtgc ttgtggacgg agcaatcgtt caggtagaga
     2641 atatgtacca tcggatccag atctggatgg aaagcggaca gaaagaacca tttgaagaag
     2701 tcaggctccg tgctctcacg gaagtaggct catccgtctt cttctctctt ctggtcattg
     2761 cagtgagctt catgcccatc ttcactctcg tagaccagga aggcaggctt ttcaaacctc
     2821 ttgcgtactc gaaaaatttt gcaatggcga ttgctgctct tctcgcactc accctcgatc
     2881 ctgcgattcg aatgatgtac aatcgctacg aattctttcg tttcaggccc gtgtggctgg
     2941 ccacactcat gagtcattta ctcgtcggaa gatactacaa agaagaaaac catcctgtaa
     3001 gccgcgtgtt gtttcgtttc tatgagcctg cgtgcaactt tgttctcaaa catcgcaagg
     3061 caactctggc cacagccgtg cttctgatga tctccaccat tccgatttat ggaaagctag
     3121 gttcagaatt catgccgcca ctgaacgaag gcgccatcct ctatatgcct gtgagtcttc
     3181 ctggaatttc gataggagaa gcaaagcgat ccctgcagat tcaggacagg atgctgcgtg
     3241 aatttccgga agtggaaaga gtgtttggaa aagtgggaag ggcagattct gcgacagacc
     3301 ctgctcctct atccatggta gaaactgtaa tcgttctgaa acctgaatct caatggagag
     3361 aagtgaagcg ctggtatagc tttttgccag aatggagcaa ggaaccgttc cgtcgcgtct
     3421 ggagagatcg cataacgtat gaggaactgg ttcaagaaat ggatcagaag attcgcatcc
     3481 cgggctggac gaatgcgtgg accatgccca tcaaaaaccg aattgatatg ctttcgacgg
     3541 gcattcggac tccggtggga atcaaaatat ctggttcgga catcaaggaa atagaaaaga
     3601 tcggtctgga gctggaaacc attgtaaaag aagtgcctgg aacccgaagt gtctatgcag
     3661 agagagtggc cggagggtat tttctcgact tcaatttgaa gcgcgaagag ctggcacgct
     3721 acggcctgac ggtagacgac gctttgacca ttgtagaatc cgcaattgga ggagaaaatc
     3781 tgtctaccac tgtggaaggg aggcgacgct ttccgattaa catccgctat gcgagagagt
     3841 ttagagaaac cctgccggat ctgaagcgta tccttgtctc cacgccctcc ggggcacaaa
     3901 ttccaatctc ttatatcgca gaggttcgat ttaactccgg gccttccatg attcgcgatg
     3961 aagacgggct gctttccggt tacgtctaca ttgatattgc aggacgcgac attggatctt
     4021 acgtggagga cgcaaagaaa cttgtaaatt ctaaactaaa actgcctcag gggaccacag
     4081 ttcgtttcag cggccagtat gaaaacatgc ttcgcgtgcg cgaaagacta cttgtagtgg
     4141 ttcctctgac actgtttctc attctagtgc tcatgtatgc gaatacggga tcggcagtga
     4201 aaacagcaat catcgtgctc gcagttccat tttcgatcgt cggggcgatc tggcttttgt
     4261 tcctgcttgg atacaacatc tccatagcag tctgggttgg catgatcgcg ctgatgggcc
     4321 tggatgcaga aacaggagta ttcatgctat tgtttctcga tctttcttac aaagaatggc
     4381 atgactccgg aagattgcag acagaaagcg atctgcgcga tgcgatcgtt catggtgcag
     4441 taaagagagt gcgaccaaaa atcatgacag ttgcctgcgg aatgatcgca ctcattccca
     4501 ttctgtggtc tcagggaacc ggcgcggatg tgatgaagcg aattgctgcc cccatggtgg
     4561 gtggccttgc tacttccttc gtgcttgagc ttttgatcta tcctgttctt ttctacatct
     4621 ggaagatcag gaccacggaa ctcaaggtcg gttgacaggg aacccttgca ggtcaggcag
     4681 ggcgaagatg caagggcaac ccaatcaact tgtcttgcga aagctgggaa tcgacacgca
     4741 acaggagtac atcgttttta tgcgctccga ttcgagcgtc tgcgtttcag aagggattac
     4801 aagtctgatg cgggtgcaga tcagctgccg cggtaagaca attgtggcca cagtcaatgc
     4861 agtgacggga aacattctgg gtcccgggga agcgtctctt tccaaccgcg cgttcgaaat
     4921 tctcggcgcg tcggacggag ataccatccg cgtcagccat ttgagtgcgc tcgattccat
     4981 gagcctcgtg cgggcgaaaa tctatggcag aagactggaa caggaacact acgatatcat
     5041 cattcaagat attgttcacg gatattattc aggagtccac ctctcagcct tcatcgcggg
     5101 ttgtgtcgca agcaagctcg atcgaggaga agttctgggc ctgacgcggg ccatgctcaa
     5161 tgcagggcag agaatgacgt ggccggggat cgtcgccgac aaacactgcg taggtggaat
     5221 tccaggaaat cgcacaactc ccatcgtggt ttcgatcgtg gcggaaaatg gaatcctgat
     5281 tccgaagact tcttccagag ccattacttc tcccgcggga acagcggata caatggaagt
     5341 catggctcct gtgaatctgt ccctcccgga aattcgcaga gtcgttgaga aatttggcgg
     5401 atgcatcgcc tggggcgggt cggctaaact cagcccggca gatgacatat tcattcgagt
     5461 agaacgcgct ctggacatag atagcgaaag ccagatgatc gcatccgttc tatcaaagaa
     5521 ggcagcggtt ggggcaacgc atgtggtgct cgatattcct gtgggagaaa cagcaaaagt
     5581 aagaagtgaa gcagaggcaa aaagactgag cgatctcttc gaagacattg gagcggccat
     5641 tgggctgaca gtgcagaccg taatcacgga tgggtcccag cccgtaggcc gaggaattgg
     5701 accagcgctc gaggctcgcg atgtactttg cgtattgcac aacgcaaacg atgcgccaca
     5761 tgatcttaaa tcgaaatcca ttctaattgc cggacaaatt ctagagcaat gcgggaaggc
     5821 aatccagggc cagggtgaag aaatggccct ggaaacacta acatccggaa gagcattatc
     5881 tcgcttcatg aacatttgcg aagctcaagg cggctttcgc gaaccgcgaa aggcacagtt
     5941 tcaacacgac atcgtttcag atagaagcgg cagaatcacg agtgtcaaca acaggaagat
     6001 cgcgcgcctc gcaaagctgg tgggcgcgcc cgcagaccct tgtgcgggca ttgaatttct
     6061 cgctcccgcg ggaacacgca tctccagggg ggatttgttg ttcactcttc acgctgattc
     6121 gcgaggccag ctggaatatg cgctcgacta ttacgcaaga caggaagact ttctacagat
     6181 gagcgaaggt gcgccatgat tctattctgt ctgggggaaa atcaaacact ggccacgtct
     6241 gtgtgtcgcg caggacagtt cgatgcgggg aatctgaatt ccagaaaatt tccggatgga
     6301 gaaacctaca ttcgcattct ggatcaggtc agaaataagc acacagcaat tctatgctcc
     6361 ctgcgcgatc cggatgcgcg catcccttct ttgttgtttc tagcagatgc gctgcgagaa
     6421 cagggcgcgg catctgtcgg ccttgcagca ccttacctct gttacatgag gcaggataga
     6481 cgatttcagc ccggagaagc aatcacatcg cggaccttcg caaagatgat cagcgctggc
     6541 tttgactggt tgatcaccat cgatccgcat ctgcacagga tctcttctct ttctgaaatc
     6601 tattccattc ctgccgtttc cctgcacgct gcttctttga ttgcggactg ggttgagaaa
     6661 actgtgcccg aaccgttgat cattggaccg gacgcggaaa gcagacaatg ggccgcagaa
     6721 atcgcccgcg cacatccctg catcgttctc gaaaaacaaa gactgggaga tagggaagta
     6781 gaaatccagg gcctcgaaaa tcttccagtg ggacgcacgc cggttctggt ggatgatatg
     6841 atctcaagcg ccagaaccat gatcgtagcc acggagctca ttcgagctca gggattttct
     6901 cccgtttgca ttgcagtgca cccgttgttt catggaagcg cgttcgaaga tttgaaagct
     6961 tccggagcgg cgacgattgc ttcatgcaat acaatcaccc atccgtccaa ccagattgac
     7021 gtaagcggaa tgcttgcctc cgcgattcaa tctatgctga atgaaatgca aaaatcatga
     7081 agcgctaaca gcagtaaggt cacgttgcac tggacttttc cacgcgtcag tttctccaga
     7141 gactctggaa tcttcagatg caatgcgaac tacaaaacca cctgttagat ccgcatccat
     7201 aggaacgtgg atcttccatc catttccggg cgctacctca agaggatttc cagactcgtc
     7261 atacatttca gaaattacga acctgtgatt gccggacggc actagaactt cgatttcatc
     7321 tccacgcgcc atgaagtttc gcacgtgaat gaatcctctc tttgtgttcg agtcataaga
     7381 cgctaccata ccgcagaaac gcgatttgtc cgaacgcgat acagccgcac catcgttgtg
     7441 agcgtctcct ggaattgtac caccaaagaa cccgggtgtg taaccgcggt tggcaagatg
     7501 ctccaaatcc tcgatgagcg attcatcaaa aggaaggccc gctgccatat catcaatggc
     7561 cttgcgatac gcgcgagtca cacgtgccac gtagtacaga gattttgtgc gaccttcaat
     7621 cttgagactt tccactccca ttttcatcaa atccggcaca tgctgaatgg cgcgaagatc
     7681 acgtgaattc aatatgtatg tcccgtgttc atcttccatg atgggcatga attcacccgg
     7741 tctcgtggct tctgtaagaa gatactcata ctcccccagt ggatgacgag gagaatcatc
     7801 gatcgcggcc ggatgaagag tgtattccca tctgcaagcg tttgtacaaa ctccctggtt
     7861 cggatcacgc ttattgaaat accccgaaag aagacaacga ccagaatagg cgatgcagag
     7921 tgcaccgtga acgaacactt caatttccgt ttccggacat tgcattttga tttcttctac
     7981 ttctttcaac gaaagttcgc gcgacaaaat gattctttct acgccttgct ttgcccagaa
     8041 tttcaccgca gcgtagttcg tagtagaagc ctgcacggaa aggtgaatcg gaagctccgg
     8101 ccagttttcg cgcactagat aaatcaaacc agggtctgcc atgatgactg catccggttt
     8161 catagcagcc acttctgcca tatctttgag aaagcttttg atcttattgt tgtgcgcgat
     8221 gatgttagat gttacataaa acttttttcc gcgcgcatga gcctcggaga taccgatcgc
     8281 gagttcttcc atcctgaact cgttgttacg cgcacgaaga ctgtaacgcg gctggcctgc
     8341 atacactgca tcggcaccaa aatcaaatgc aatgcgcatc ttctcaaggc tgccggctgg
     8401 caagagcaac tctggctttt tcataaccag gtttgccggc atccctcgca gaagcaatca
     8461 atagcccaga aactcttcgc tgtgtatatc gtggtcaaat acgcccatct ggaccaggag
     8521 ttcccgcatt ccagtcacca tacgagaagg gccggccaca tagaacaccg gataggcgtc
     8581 ccggggcaca tacttgcgaa tcattacttt atcgattctg cctctctcat acttccaccc
     8641 tggactatct gcttccgtca aggttggaat gaaatgaaag tttggaaatt cagcagcata
     8701 cgtttcaaat tcgtctagaa atgccgccag ctccagggaa cgaatcgaat agaacagata
     8761 cacctctgtt cccggcttct cttttagaac ccagctcacc atgctgtgaa acggagtgat
     8821 tccaattccg cctgcaataa acgcaagagg ggccggagta tttttgattg tcatctcgcc
     8881 catggctgga ctcacgaaga tcggagcacc ttcgggcagt ttcatgagag cattcttgaa
     8941 gggactgttg cgcatccgcg atacgaacat cagcgcgtcg gatcgatcag gcgaagaggc
     9001 aatggagaac gtgcgagtcg attggcccgg cacagattcc atctctggca gagtcaaatc
     9061 cgcgaactgt ccgggctcaa acgcatagtc gttcagcgat gtatcgagca agaatgccca
     9121 cgtgtcctga gcaatctgca tcttctgcaa cagcggaagc tggtactgtt cagacgtccc
     9181 gccctgaccc ggagctactt ccacaatttc gtagagcttg aatctgcctg tcttcccttt
     9241 caacggagcg ctgaatactt ttccacgcgt cacctggtcc ttcacgtgct cgtagaccgc
     9301 atctgaaatc aaaaacggaa cgccagcctt cttggttgca gattcaatcc tgcttgccat
     9361 gttcacatta tcaccgagtg ccgtgaactg cactttcttg gggtgtccca tttctcccac
     9421 gatcatctcg ccgtagtgaa cgcccacgcc aatgttgaaa ttcacctgga acgttctctg
     9481 caaatatcga ttcacatgtt ctaattccgc gagcatggat gaaccagcgc gaacagcatt
     9541 caagcaggcg gtgcgagcgt cagatgaatt caatccgaag attgccatca atccgtctcc
     9601 aatgtactta tcgatgatcc cgccatttcg caagatcact tctcccatcg cattgaagta
     9661 tcgattgaga atgtgaacaa catcgtacgg aagatgattc tctgaaaaag gagtgaagct
     9721 gcgaatgtcg ctgaatagaa tcgcgagatt catttctcgc cccgtcgtgt gaatggacgt
     9781 tcccacaatt tcgcggatgt catcctgatc aatgaccaga cgacgaaccg taacatctcc
     9841 atgcgcgcga gtctggcagg ccacgcgaat gtcgtccgtg aagccttttc tgagtgcgag
     9901 tgccgtttca gcttcgttgc gtggttcgag gttttctgct ccctttagaa ccagtattct
     9961 acacgtagaa caacgcgcat ttccaccgca aacgtgcgtg tgcggtattt catgtttcaa
    10021 ggagatttga agcaaactca gagacgagtc tgtctcttcc actgtgcaat cgttggtgta
    10081 tgtaatcttc aaagtcaatc tcccagaaag aatcgaacga tctcttcgct cgcagaaaaa
    10141 tcacgcgacg tgcgccccac aacaacttcc ggtagatact gcaaacctcc cggccatgtg
    10201 tgtcccccgc caagcactcg tatcaatctg actcgagcat tcccgcaaga ccaggtttcc
    10261 gctgtcgcgc ttgtgttgtc tttgacagca tccggaatat ccgtcttcac agaatccgca
    10321 ttgcactggt tatgcgatcg ccagaactgc agagtggcat ccgcggacag gactctaccc
    10381 ctggtcttct tgaacaaaga gatgtccccg ccgccgtaag ggacaagagg atcttccgtt
    10441 ccgagtatga acagagtaga aatgggtgcg gacggtttac atttaggcgc aaatgattcc
    10501 tggagattgg cggccacgga tgcgatcgcg cgaaacgccc gactttcgca tgcaagccgc
    10561 tgcgacatca ttccgccgtt ggaaatgcca gtaataaaaa tcttatcttc agggatcttg
    10621 tagtccgcgg cgagtttgag cgccagagaa tccagaaacc ctgcatcgtc tacgttctcc
    10681 gaaatggccg tggatatctt agtatcgcgc ccgtcgttcc atcccttgtc gattccctga
    10741 ggatacacca gcagaatatg tctttgcttt gcgatttcag gcagaccttc cgccagtcgt
    10801 atcatatgtg ctgctttgcc gccaccgcca tgaagcacga agatcatgga gcgaggctgc
    10861 tccacgggta ttaccagata ctcgcgagcg cgtccgccat attctagctc atgccgagtg
    10921 gccgcgctcc tgcaggaaag tagaaatacg aaaatgctga ttgtgagaca gacgcgcatc
    10981 attccccctt accagactgg ttttacaaac agcctcatct cttctacatc cagacgttct
    11041 agctccagat tccacacttc gcccagaaac gcaggccggc tgagcttctt gatgtatgca
    11101 gaaaaaggat ctggaagaat caattccata tctcgagtga gatgcatatg cagtacaatt
    11161 gcttctacac ttacgtcagt caactccaga aacactctgt ccgggcgaaa accagtaatg
    11221 aaaccgcgaa agcttttttg tccgcttctc tcaatggatc gaagcatctt cagtttccag
    11281 atatcgcgct ctgcgtccat cgccttgcgt tccatctcgc tagtatgaaa cccgagttca
    11341 gaaatttcag cttctgtgta tgcatgcttc tttttctgga tcaggttttg aagagcgcga
    11401 tgcacaacaa gatccggata cctccgaata ggagaggtga agtgacagta gtcgcgaaag
    11461 cccaggccgt agtgacccag aggctctccc atgtactttg cctgcatgaa ggatctgagc
    11521 atcaacatct gaaagatgcg atcgggcggc acaccttcca gtttgcgcgt cgatcctttc
    11581 tgtacgcttt ttcctttctc tgtgaattca tgaacaacct tcagcgcatg attgatattc
    11641 tccggatctg aattcggaag ttctagattg attccataga tcttgaggaa gtgattgagc
    11701 gcttccagct tcatgtcgtc cattggttcg tgaacgcgaa acagtgtatg cgcttccttc
    11761 tttctctgga attcagcggt gcagatgttc gcagaaagca tgcattcttc aattaacatg
    11821 ctggacttca accgctcgcg atacagtact tcttttattc taccttctgc atcatagatc
    11881 acttttggct cgcgcatatc aaagtcgatg cgccctgctt tcaatcgcgc agccttctgt
    11941 gccttcgcaa gcgaccagac tcgcccaagc atgctgtcgg attcatccag catggtttct
    12001 gccagatcgt aggtgagccg cttgtccacg cgtatcacgg acttgtagat ctgatacttt
    12061 agaatctccc ctgtgtctgg acggacgtcc atttcgcagg tgaatgccag tctgttcctt
    12121 tcagcgatga gcgaacaaaa gttttctgaa agaacaggag gcaacattgg taccactcga
    12181 ttggcaagat acaccgaggt agctcgacgc gccgcttcac gatccaggtc tgttccaatc
    12241 cttacgtaat gcgaaacatc tgctatatga acataaagtt tccagagccg cggagactct
    12301 accttcagac tgatcgcatc atcaaagtcc ttcgagtcag gcccgtcgat ggtgaccgtg
    12361 taaagatccc gtaaatcaat gcgtgaattc caatcaaaga cattctccgg ttttacttct
    12421 gaagcttcct gatcatattc cggtaaacct tctacttctg gatacaccgg atcgagattg
    12481 tgcttcatga gaacacgcgc ataatccgga tccatatccg tatcgctttc aaaccgaaca
    12541 aactcagctt ccggcaaagg tagacccatg tgcatgatgg tcttcccagg catgcgaacc
    12601 acaacgacca catcttgctt catcctgctt accagatccg caggtagtcg cggggtcgcc
    12661 aaacaggccg ttatgcgaga gtgtatatca agaagaacgc caggaattcc gcgatgaact
    12721 ggttcgctga gcaacttcat tcgataaagc tcacgcgctt tctcccgaat ctcaatgacc
    12781 tctccttcga atcgatctct ggttttgtct gtgagcttca cgagaacaag atcccccgtc
    12841 agtgcattca tgcttttggc tgccggaatg aacaaatcac gagacgcagg atctgcccct
    12901 ctcacggcac caaagaccgc gccagaagga gaaagagatg ctttggctac ggccgcaaag
    12961 ggactcgcag gaatgatctt attgccatcg gttcgtacca ggttcagtag ctttagttcg
    13021 gcgagcagta cgtcgacttc ggattcggcc tggtaacccc ttacctgccg actttttcta
    13081 aaagattttg cttttgcttt gaacgcgccc gtgctgctaa aagcagacaa gacttctttg
    13141 cgagggagct tacgccctgc attttgttcc aggtatcgca acaatcgcga tgctgtactc
    13201 attttatatt aaatcctcca ggatacctgg cctcggccgg atccttacgt aaatagtagg
    13261 ggaacaattc ctgataggag cctgacgccg gcatctccga atgcgcggca aaaatccatg
    13321 ccgcctccgg ctccttcagc tgacgcgaat ctcctccgtt gcgagcgcga aacgtatccg
    13381 ggtcatcaca atacaccggc aaacctgacg cggcaacttc ctctgcggtt aaatcctgta
    13441 cgggaatcag atccatttgc gtagcgctga gtccagactc tggaattatc tttgtataaa
    13501 aacggttttg ttttccgtca atgcacaccg cgaagggacc tggctccata tttcgaatcg
    13561 gcagcgaata gcacgtcaaa gttccgatcc ctcttacagg tacattccag agttgcgcca
    13621 gatctcttgc tgcgctcacg caaatgcgaa tccctgtgaa tgatccagga cccgtaagag
    13681 tcacaatctc tgaaggtcgc ttaacgttag cggcagataa gacttcctgg atcgcaggaa
    13741 aaagattctg gaacgcttcg cgcggagctt ttcgcacgat ctgatattga ttctgttttg
    13801 aaaaaagacc gactacaatg tgggtgtgag tggtatcaat gcaaagacag taattgttca
    13861 ccctgccttc tatagaactg aatgtgcctg aggctgtcat ccttttcatc aatggaaatt
    13921 tcgaccagaa atacgggaac tccgggccca atatgaggta gagcgcgatc tccccactct
    13981 atggcgtgca cggttggagc gtgatcgacg cgatgccagt cttccagaaa tcccaactct
    14041 tcgagctctg cgactttctg caatcggtac aagtcgtaat gatgaagcac tccgcgcgac
    14101 ccttcatatc gattgtgcaa attaaatgta
//
LOCUS       DAOQJL010000024        14122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2085_length_14122_cov_4.111964, whole genome shotgun sequence.
ACCESSION   DAOQJL010000024 DAOQJL010000000
VERSION     DAOQJL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_14122_cov_4.111964"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLG78_01930"
     CDS             complement(<1..1273)
                     /locus_tag="PLG78_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE21893.1"
                     /translation="MQPASSESSELKKFVHVCPACQNRYRVTIQVKVYKVHRAACPVC
                     GNVNYFDNRRGLLDRQGNASAPRPAQDVVYPEPPTTKKKIKTRGILDGPLFWTRGSKS
                     TGASRRFSTWSLPELSGRTLILGGAFLGLIPIVMFLALQIVPSVFLPEGGDTYLSGLT
                     TIRENRILDRKGRLVGELFSRKTGNLKYSEIPGSFRDILLFVEDEHFYSHGGVHWPSV
                     VRAFARNVTSMGYSQGGSTLTQQLARILLNARQKTLLRKVRETSLAYYIEGHMSKEDI
                     FTAYVNQVYLGHGAVGADNAARFYFLKGIADLNFAQMLALSCLPSAPEMYSPLRYPDR
                     IERKMDAVFARMKSEGFSGAAEITQEQYESQKAAMLSNTTRSPSETVFGNREDIAPYV
                     SEHVRNKIGELFGVEQEFGAGLQIETTIDADL"
     gene            complement(1245..2405)
                     /locus_tag="PLG78_01935"
     CDS             complement(1245..2405)
                     /locus_tag="PLG78_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE21894.1"
                     /translation="MKRRILLGLFGLSYLGLALWAFPDIRQTIRLHVLSWFGAASTGG
                     PTIAEDIFLSQFRPDPMLKTRQTILSHPFIPTVEFHGHLFTSPTSDPPEDILTRMDQT
                     HTSVFVNLSLFTSTPHSYQKLVEKQPSKRIFHFVGFNWKRMSEGPDFAQKMASDLEQI
                     AKMGGRGVKLWKNFGLMQKYEGKLVTLDDPRLDPVWDVCAKYHLLVAVHTADPPVFFL
                     PVDEHNERFEELGRRPEWSFHKPGLPSFDDVMQQRDRLFTRRKDVTFVALHFGEFAHD
                     LSRAQDLLDRHPNVYVDIAQRIDELGRQPRAAREFLIKNQNRILYGTDGLPDPEKLKI
                     YWRFLETDDEYFDYFPAGTNRKGVWKIYGVKLPPDVLRKIYYSNAARLLRIQ"
     gene            complement(2402..2617)
                     /locus_tag="PLG78_01940"
     CDS             complement(2402..2617)
                     /locus_tag="PLG78_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNE21895.1"
                     /translation="MAILKDIEKGAGEIIRVEVSEYQGKKYLNVRVWYTDKTSGEFKP
                     TQKGVAIRPDQFDEFLSAINAARAELQ"
     gene            2671..2874
                     /gene="rpmB"
                     /locus_tag="PLG78_01945"
                     /pseudo
     CDS             2671..2874
                     /gene="rpmB"
                     /locus_tag="PLG78_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
     gene            complement(2972..3400)
                     /locus_tag="PLG78_01950"
     CDS             complement(2972..3400)
                     /locus_tag="PLG78_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE21896.1"
                     /translation="MSSRFAVAIHILTVLGLCTDKPVSSELIASSVNTNAVHIRRILS
                     NLKQAGLIRTTRGSQGGYLLARSTGEISLRQVHCALGENLFHFPRSTPNKECPAGANI
                     RSSLETVFGGATEAAHAHLDQTRLAHLVDEIREKSGQKGA"
     gene            complement(3493..4341)
                     /locus_tag="PLG78_01955"
     CDS             complement(3493..4341)
                     /locus_tag="PLG78_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21897.1"
                     /translation="MRKIALLAFVGLLAGDLSAASYKLFVHGRSGSNHCQALTSTASG
                     TTDYNNYWGGAVTGLTNVRYVGFDGTQSGGAYSWNNCGAQKQLNDAIRIFCANGNSCE
                     IYTHSTGGLVAAAYFALNNPSGISVTRIQLLASAAGGSELADFSTSYLSWLGFDTLGG
                     QLDQSVSTSGARNGFNHNTSNGRTYYTTSGEGTDYLYATSPLLPGYDDGVLANHSLCS
                     VNRVTAVGTSCTRGSARMTESYKCGFLWLSTCYYNYDRWTPYYTVWQGGSSESHGKAK
                     YDYNRR"
     gene            complement(4440..5051)
                     /locus_tag="PLG78_01960"
     CDS             complement(4440..5051)
                     /locus_tag="PLG78_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE21898.1"
                     /translation="MASTEITLMDLGRRGCQGTPRDFAAWVHFETGVEDPSVPAVLDN
                     LVSAGKFKHHPGFDHEYILASPIEFQTSVRYKVSGELKQSPIQMVRSCMEAFLRSQGT
                     ADETLMDISIATTEAMENAVKYSDHSPIEVSYSVENGVFHIMIKNRLSEVRLEKDIEA
                     GKYTSTTTLMRGMMVMVKLFDEMDIEILEEEKLAVFQAHKRIK"
     gene            5146..5871
                     /locus_tag="PLG78_01965"
     CDS             5146..5871
                     /locus_tag="PLG78_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNE21899.1"
                     /translation="MGLVKISIFQKSIHEPLGPSTFKKLTGLKSDFLVLPEYLFADAS
                     VKDQASGIEKSKQALDWLLKLSDSYKGIIIGGSVIIEEDGKLYNATPIVSDSKIVDFY
                     RKRNLTQNETKHLTAGTEAGVFILGGFRFGVLICADVLEPKLIDELAEMGVKLVFAVM
                     NSPYREETVEEKKARDEKLFCAPAAKHGLVIAKCCGVGTTFGRKIQGRSLVVTPTGIS
                     WRVSLGEEDSELLKTILVQAPSV"
     gene            complement(5853..6782)
                     /locus_tag="PLG78_01970"
     CDS             complement(5853..6782)
                     /locus_tag="PLG78_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21900.1"
                     /translation="MHHLSDLKGQPRARSILEAYLQKTPPPLLILSGPAGTGKHSAAE
                     IFVQSHFCDTGSACGQCGPCRRMLKGEHPDYIRFPEDRIKIGDRRKPDDFTIRWLIST
                     RLPYRPHEAPFRFVLFPDASLIQDEAETSLLKTLEEPPEHTRFIFLVRDVSELKPTIL
                     SRGLLVPFQLLDRVSLTEVSGVDSPDLLDLLAGSLENLEFFQSELYRSLKSRVADTSS
                     RPGLLELEKWMIDLDRGGAEKMLGAPIESMRLWSLLVLVWLRHLNADVSGNLRRLTAL
                     LDFQGDLRSEMPGFQSYAISRMFHRMACYTEGA"
     gene            6977..7591
                     /locus_tag="PLG78_01975"
     CDS             6977..7591
                     /locus_tag="PLG78_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21901.1"
                     /translation="MRSIFLLLLMFAVFTGCKSGDEAPQETSPLVSLLGLNLVTGYGW
                     TGRLTSQTCSADTIQSTYVSCPVFNGLEAQTVAAVPNNATLVMQLYNNRLSGQFTLIL
                     PHYNFIPTNLYQITTSMTTEQGYDTGSGAVTTVRQAFSNTVPYTVTYTTQSTNTTQVD
                     LLDFAATQDPNYLSGSMKLKFTQPSNAAAGQIIATYGFKFDKLF"
     gene            7599..9026
                     /locus_tag="PLG78_01980"
     CDS             7599..9026
                     /locus_tag="PLG78_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE21902.1"
                     /translation="MMRTLFALLLALTAVQLNAVEPEKDPPVETPKKKGGDPGLEGFK
                     VTGLPLISFSTDDGFGYGVRAYGTYYEEGYAPFKFQAFAQYYKTTRGYEYHEVSLDAL
                     KFLGTPLRLRLDGGFERTLNAQWYGYGNYHDLARIKQIKNGEIPINENTPPNTKRDLY
                     QYDQNITLNNLALTNPFGSGNQIFNPSQRVLRESQDKYFYYDRIRPFINISTEDFIGD
                     TNFKWFAGVRGQRYKIQSYQGDYDNGNTVANIKTLIDNERPYGYDATQDRKYVNGVRG
                     AIAYDSRPRDRESNPNGGIFTDIHVEGVGKGTGSHYSFTRTTVTWRQYFDVAPSFFNS
                     FDKELVIGYRLLGQETSGDVPFFEAGRIYTMRESAEGLGGNGGVRGYSANQFVDKDMV
                     IGNLEMRLTTFKISALGGMDFVLLGYHDEGRVGASRREIDMDNWHKANGGGVRIVWQK
                     NTIINISYGRSKYGANGNFSFNHLF"
     gene            9104..9544
                     /locus_tag="PLG78_01985"
     CDS             9104..9544
                     /locus_tag="PLG78_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21903.1"
                     /translation="MRHFLPLFFLLATAVAAQSEKGIDVVQRANGVFIYRYDETLEQI
                     DPRKDFKAKKQITEQSVIADLKRLLKDNTDYTPNFKARCMPVWDAGIEFRTPTETRMY
                     LFSFRCNTVKSVEDNTFKDFTPQRTELYGLLMYEINDRTSYSGR"
     gene            9516..10475
                     /locus_tag="PLG78_01990"
     CDS             9516..10475
                     /locus_tag="PLG78_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNE21904.1"
                     /translation="MIGRPTVAVRRHLLLLLVLATPLWSQAPRQPVIAILPFQAKDSA
                     RGEELASRLTAEIVARQAFPVAERAQLKKALEELAKGQTGLFQPENAPQLGRMIGADY
                     LILGEILDSTDKGGVRASMRVVRAETGIVIGAAVTGGEADAVSRDLSLSAIKTVSIHA
                     MLENPDSPYSVLLLLDKGKNPEYKLGETLRLKFKVLKHDQTASEKVYLSIYSINADGV
                     MTLIYPNRFSGQEGVYVGREYTFPDPRDDFEWKLVPPAGAETLQAIVSNKPVRLANER
                     ATEDFPVLGVKESPALYRGIQTQLKEEKRGNWAAERITYILKP"
     gene            10525..11763
                     /locus_tag="PLG78_01995"
     CDS             10525..11763
                     /locus_tag="PLG78_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21905.1"
                     /translation="MTLDLQLPRNESQNIPRLLNGSAGQVLSWNLLYSDPQTSYYRIE
                     LFGTSAPEELEAHILEGCPQARVKIKDDTAHGSDALLAIGPARSLEFRKAFHRTSPEV
                     VENSASLLLKNREDARELPMVKERALFISDGGALRVKNQALLALERDGYLACKFSGMQ
                     AIPLLLDARNEEEFIKSVMALAGNVSVIRLSMLQRTMSRQIVERITEQASIPVIHAEY
                     LESSATIAAIVCNALQYHSMTLDEKVVAIVGLGPCALGLSRILSRMGASKLYGVDTEY
                     ALNSRFEKDGGTATSLDFALERSDVVVISPGTQVLIDPKRLRENQLVLSFAPSAIDMS
                     ALKPEVSRSVHLCEEPHPIYALPGLLAGVHAGAKADIEISIRIAEVLVGRCGDNRFLP
                     IPSESLVRAEAEAVSLTSRR"
     gene            complement(11798..12850)
                     /locus_tag="PLG78_02000"
     CDS             complement(11798..12850)
                     /locus_tag="PLG78_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE21906.1"
                     /translation="MTLRTIRLILFITLIPEVLILGFFVYARIQSPPKAGRMIANALG
                     IPHPAVIAHRGASYLAPEETRAAYLIARDLGADYLEGDVQRTKDGILVLLHDDTLERT
                     SNVAEVFPGRQKDTIDRFTYAELLKLDLGSWFNSANPDRANDRFKGLKIQTVEDLLAI
                     AEGGNHRPGVYLETKSPERYPGVEKDLVDLLTKRGWIGRDRVCADTPALIDESVIRPV
                     RVGCGKAAVIFQSFSAESVQVLKNIAATVPRVYLVDEEMGARDGWPALVEKARSLDAG
                     MGPVGYLSYPWHTGPAHRAGRLVHSYTINKKWQLWLMNQFGSDGVFTDRSEMALAHFD
                     RVPPLDTKKLIAESVF"
     gene            complement(12903..13892)
                     /locus_tag="PLG78_02005"
     CDS             complement(12903..13892)
                     /locus_tag="PLG78_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21907.1"
                     /translation="MIRAWCLLILLFSLQVYSEPTELERYLEWAKYPPNSRPLEQTQV
                     DLLDPYNSDRPPVPVSGLSAACTLIPQSAVSVGTKEFKIYLHCKSSLGQPLKVDSVKA
                     RVYTEDRGKQKASAAPRIDAGNRPDQSIQFLVKPGVQDWGDMFLEADFNVQGKRHNQR
                     AHWFSTPHNVAEFKQGATESVRQGHLYVRIPVWIHKAGYFAFDANLFSKSGPVATSSV
                     EGDYSAGLQTIEFQFFGKVIRDKKAGGPFTIRNIRGMRNNSPVTPSMVRDSINGKPLP
                     PHKEQTEPWQEFMEPFAGAVTTAPHSLAVFSSLEWQSPEKTRRIQFLRGLEKK"
     gene            complement(13894..>14122)
                     /locus_tag="PLG78_02010"
     CDS             complement(13894..>14122)
                     /locus_tag="PLG78_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21908.1"
                     /translation="NDGAFDRSSGKPVNASGGALSGGIIYASGLARLMEASLQVTGQA
                     GPSQVPGAKTALVSAQAGLGIQSSIIYVVEG"
BASE COUNT         3633 a         3674 c         3451 g         3364 t
ORIGIN      
        1 gcaaatccgc gtctatggta gtttcgattt gcagaccggc cccaaattct tgttccacac
       61 cgaatagttc cccaatcttg tttcgcacgt gctctgatac atatggagcg atgtcctccc
      121 gatttccaaa tactgtttca gacggactgc gagtggtgtt ggaaagcatg gccgcttttt
      181 gcgactcata ctgctcctga gtgatttctg ccgcacccga aaatccttct gatttcatgc
      241 gtgcgaagac agcgtccatt ttccgttcaa ttcgatctgg atatcgcaga ggcgaataca
      301 tctcgggtgc agaaggaaga cagctgagcg caagcatctg cgcgaaattc aagtccgcga
      361 ttcctttcaa gaaataaaaa cgagcggcgt tgtccgcacc aacggcaccg tgtcccaggt
      421 acacttgatt cacgtatgca gtaaaaatgt cctctttgga catgtggcct tctatgtagt
      481 aggcgagaga tgtttcgcgc actttgcgta gcagagtttt ctgtcgcgca ttcaaaagaa
      541 ttcgcgctag ctgttgcgtt agagtagagc ctccttgaga ataccccata gaagtaacgt
      601 tacgcgcaaa tgctcgaaca acggaaggcc aatgcacgcc gccgtgcgaa tagaaatgct
      661 catcttctac aaagagaagt atgtcgcgaa aagatcccgg gatttcagaa tacttaagat
      721 tgcccgtctt tcgcgaaaac aattccccta cgagccggcc tttgcgatca agaattctgt
      781 tttcgcgaat ggtagtaagg ccggacaggt atgtgtctcc gccttcgggg agaaagacgg
      841 aaggaacaat ctgcagcgca aggaacatca cgatcggaat caatcccaga aacgccccgc
      901 cgaggatcaa tgtccgcccg cttagttcgg gaagagacca tgtgctgaat cttcgcgatg
      961 cacccgttga tttagaaccg cgcgtccaga aaagaggacc gtctagaatc ccgcgggttt
     1021 tgattttctt ctttgtggtt ggcggttctg gatagacgac gtcctgcgct ggtctgggcg
     1081 cacttgcgtt cccctgtctg tctagcaaac ctctccgatt gtcaaaatag tttacgtttc
     1141 cacatacagg gcatgccgcc ctgtgaacct tgtatacttt tacctgaatc gtaacgcggt
     1201 atctgttctg gcaggcaggg cagacatgta cgaatttttt cagttcactg gattctgagg
     1261 aggcgggctg cattgctata gtagatcttt cgaagcacgt ccgggggcaa ttttacaccg
     1321 tagatcttcc aaactccttt tcggttggtt ccggcaggaa agtaatcgaa atactcgtcg
     1381 tctgtttcga gaaaacgcca gtaaatcttc aacttttctg gatcaggaag accatctgtc
     1441 ccgtacaaaa tgcgattctg gttcttgatc agaaactctc tagcggccct gggttgacgc
     1501 ccgagctcat caattctctg cgcgatgtcc acgtagacat tcggatggcg atccagcaaa
     1561 tcttgcgctc gcgacagatc gtgcgcgaat tctccaaagt gaagtgccac aaaagtgaca
     1621 tcctttcgtc tggtgaacag tctatctctc tgctgcatca cgtcatcgaa tgagggcagc
     1681 ccgggcttgt gaaaagacca ttctggtctt cggccaagtt cttcgaaacg ttcattgtgc
     1741 tcgtccacgg gcagaaagaa aacaggtgga tccgcagtat gaactgcgac gagcagatga
     1801 tacttcgcgc agacgtccca gaccgggtcg agccttggat catccagcgt caccagttta
     1861 ccttcgtatt tctgcatgag cccgaaattt ttccatagct tcacgccgcg gccacccatc
     1921 ttcgcaatct gttcgagatc agacgccatc ttctgtgcaa aatcgggacc ttcgctcatt
     1981 ctcttccaat tgaatccgac gaaatgaaag atccttttgg atggttgttt ttctacaagt
     2041 ttctgatagg agtggggcgt ggatgtaaac agagacaggt tcacaaacac agatgtgtga
     2101 gtttgatcca tacgagtgag aatgtcctct ggaggatcac ttgtcgggct ggtaaaaaga
     2161 tgtccgtgaa actcgacagt cgggatgaaa gggtgagaga gaatcgtctg acgagtcttg
     2221 agcatcggat ccggacgaaa ctgagaaagg aaaatgtctt ccgcaatggt gggaccgcct
     2281 gtggaagcgg ctccaaacca ggacagaaca tgcaaccgaa tggtttgacg aatgtcggga
     2341 aacgcccaca gagcgagccc caggtaactc agtccaaaca acccgagcag aatgcggcgt
     2401 ttcattgtag ttccgcacgt gcagcgttta tggccgacaa aaactcatcg aactgatccg
     2461 gccgaatcgc aacacccttc tgggtgggct tgaattcacc ggaagtttta tccgtatacc
     2521 agacccgcac gtttagatac ttctttccct ggtattcgga tacttccacc cgaatgatct
     2581 ctccggcgcc tttttcaata tctttcaaga ttgccatgat taccaccctt ttgaattgac
     2641 cgatcgtcct tccagcgaaa tcctggccaa atggcgcgca gatgtcaatt gtccggtaaa
     2701 caatctggtt ttggagctca ggtttcccac tcccacagag tcacgaaacg caaattccag
     2761 atcaacctgg tagagcgccg cgtcttcctc gaagacgaaa atcgctgggt gcgcctgcgt
     2821 atttccgcaa gaatgctccg cactctgaac aagaaaggcc cgaaggcaat catttcaaag
     2881 tataaaatgg atccgtccgt tttgaaagct gctaaagcac tttcagccaa gccagcgccc
     2941 caggcaaaag gttcagccgc ctgatttaag atcaggcgcc tttctgaccg cttttttctc
     3001 tgatttcgtc cactaaatga gccagcctgg tctggtctag atgtgcatgg gctgcctctg
     3061 tagctccccc aaatactgtt tccagcgatg atcgaatgtt ggcgcccgcc gggcattctt
     3121 tgtttggagt actgcgggga aagtgaaaca gattctctcc gagagcacag tgcacctgcc
     3181 tcaacgagat ttctcccgtg gacctggcca ggaggtatcc accctgactg ccccgagtgg
     3241 tccgaatgag accagcctgc ttgaggttcg agagtatgcg tcgaatatgg acagcgtttg
     3301 tatttacgct cgaagcaatc agctcagagg aaacgggttt gtccgtgcaa agacccagaa
     3361 cggtcaggat atgaatagca acagcaaaac gcgacgacat ttgtcaaagg tttggaagcg
     3421 acactcgcac tcaaagcaaa aaaaagccgg gaggaaattc ccggcttttt tctcgatctc
     3481 tttgaattag aatcagcggc gattgtagtc gtacttggct ttgccgtgcg attcgctgct
     3541 tccgccctgc catacagtgt agtatggagt ccagcggtcg tagttgtagt agcaagtgct
     3601 cagccacagg aatccacatt tgtaggattc agtcatacga gcagatcctc tcgtgcagga
     3661 agttccaact gcagttacgc ggtttacgct gcaaaggcta tggttagcca gaacgccgtc
     3721 atcataacca ggaagcagtg ggctcgtagc atacaggtaa tctgtgcctt cgccagaagt
     3781 agtgtagtaa gtgcggccgt tagaagtgtt gtggttgaat ccgttacgcg ctccgcttgt
     3841 gctcacagac tggtcgagtt gaccgcccag agtgtcgaat ccaagccagg aaaggtaaga
     3901 agtggagaag tcagccagtt cagaaccgcc cgctgcggat gcaagaagct ggattctggt
     3961 tacgctgatt ccagatggat tgttgagtgc gaagtaagct gctgcaacca gtccaccagt
     4021 agagtgagtg taaatctcgc agctgtttcc gtttgcgcag aagatgcgaa tcgcatcgtt
     4081 gagctgcttt tgtgcaccac agttattcca gctgtatgca ccaccgctct gagttccgtc
     4141 aaagcctacg taacgaacat tggtcagacc agttacggct ccaccccagt agttgttgta
     4201 gtcagtagtt ccgcttgcag tagaagtaag agcctggcag tggtttgatc cagagcggcc
     4261 atgaacgaac aatttgtaag aagcagctga aaggtctcca gcaagcaatc ctacgaacgc
     4321 taacaaagct attttacgca tgttatcccc ctagcgtagt agtagtttat ggtgcaccgt
     4381 gccatagatt cgtgtacgtg caactcattt ttgaacctat cggcaaaaaa acggtacgtc
     4441 tacttaattc gtttatgagc ttgaaaaacg gccagtttct cttcctcgag aatctcaata
     4501 tccatttcat caaacagctt caccatcacc atcattccgc gcatgagtgt ggttgtactg
     4561 gtgtatttgc ccgcttctat gtccttctcc agtctcactt cactcaagcg gttcttgatc
     4621 attatgtgga atactccgtt ttcgactgaa taggagactt caatagggct gtggtcactg
     4681 tattttacag cgttctccat tgcttccgtt gtagcgatac tgatgtccat caatgtctcg
     4741 tccgcagtgc cttgcgatct tagaaatgcc tccatacaag atcgcaccat ctgaatggga
     4801 gattgcttta attccccgga tactttgtag cgcaccgacg tttggaattc tataggagat
     4861 gcaagtatgt actcgtgatc aaagcccgga tgatgtttga atttcccggc actgaccagg
     4921 ttgtccagaa ccgctggaac ggacggatcc tctacgccgg tttcaaaatg aacccatgcc
     4981 gcaaaatccc tcggtgtccc ctggcagcca cggcggccaa gatccatcaa tgttatctcg
     5041 gtgctcgcca cttcgcctga atagaggatt tatagaatct tgtccaggat tgcaagtaat
     5101 aaagatcgtt cttgacagcg cgtcatgctt tgcgcagcag atactgtggg cctggttaaa
     5161 atatcgatct tccagaagtc cattcacgaa ccactcgggc cttctacatt caagaagttg
     5221 accgggttga aatcagattt cctggttttg cccgaatacc ttttcgcaga cgcatccgtg
     5281 aaagaccagg ccagcgggat tgagaaaagc aaacaagccc tggactggct cctcaagttg
     5341 agcgattctt acaaaggaat cattatcggt ggcagcgtca ttatagaaga agacggaaag
     5401 ctctacaatg cgacacccat cgtctcagat tcaaaaattg tagatttcta tagaaagcgc
     5461 aatcttacac aaaatgaaac taaacatctc acggccggga cagaagcagg cgtctttatt
     5521 ctgggagggt ttcgctttgg cgtgctgata tgtgcggacg tacttgaacc aaagctcatt
     5581 gacgaactgg cggaaatggg tgtgaaactg gttttcgccg tcatgaattc tccctatcgc
     5641 gaagaaaccg tggaagaaaa gaaagcacgc gatgagaagc tgttttgtgc acctgcggct
     5701 aaacatggac tggtgattgc gaaatgttgc ggggttggaa caacgtttgg tcgtaaaatt
     5761 caaggacgtt cgcttgtcgt gactccgaca ggcattagct ggagagtgtc gctgggagaa
     5821 gaagacagcg aactgctaaa aacaattctg gttcaagctc cttccgtata acaggccatt
     5881 cggtgaaaca tgcgcgaaat cgcgtatgat tgaaacccgg gcatttccga tctaagatct
     5941 ccctgaaaat caagaagcgc cgtcaatcta cgcaaatttc cggatacgtc tgcattgagg
     6001 tgtcgaagcc agaccaggac cagcaaactc cacagacgca tactctcaat gggtgccccg
     6061 agcattttct ccgcacctcc acgatccaga tcgatcatcc atttttccaa ctcaagaaga
     6121 cctggccgcg aagacgtatc tgcgactcgg ctcttcagtg atcgatataa ttcagactga
     6181 aagaactcaa ggttctcaag actgccagcg agcaaatcca gcaaatcggg cgaatcgaca
     6241 ccggatactt ctgtcaaaga aactctgtcc aacaattgaa aaggcaccag aagtccgcgg
     6301 ctgagaatcg taggtttgag ctcacttacg tcgcgcacta gaaatatgaa acgagtatgc
     6361 tccggaggtt cttccagagt cttgagcaac gacgtttccg cttcgtcttg aattagactg
     6421 gcatccggaa ataacacaaa gcgaaagggc gcttcgtgag gacgatacgg caaacgcgtg
     6481 gaaatcaacc agcgaatggt gaagtcgtcc ggctttcgcc tgtctcctat cttgatgcga
     6541 tcttctggaa atcgaatgta gtcgggatgt tcgcccttga gcatgcgacg acagggcccg
     6601 cattgaccac aggcggatcc ggtatcgcag aaatgactct gcacgaagat ctctgcggcc
     6661 gagtgcttcc cggtcccggc cgggccactc aaaatgagca aaggcggcgg ggttttttga
     6721 agataggcct ccagaatcga tctggctcga ggctggcctt tcaagtctga caggtgatgc
     6781 atatgagttt aaaaacgaaa cccggcgcga acgccgggtt acactcttcc tgccccaatt
     6841 tctttggcta acacaacagg caccggtccg atgagtaaaa tcctttttgg gacatgacga
     6901 ctattttttc aacatcccga tcaaaatttc gccttttcta ttgactgatt tttcagtcgt
     6961 gcgttttcaa gtgcccatgc gaagcatctt tctgctcctt ctgatgttcg cagtcttcac
     7021 aggctgcaag tccggagacg aagcacctca ggaaaccagc cccctcgtat ccttactcgg
     7081 actcaatctt gtgaccggct atggatggac gggaagactc acgagccaga cgtgctcagc
     7141 agatacaatc cagagcacat acgtatcctg ccctgtgttt aatggactgg aggcgcaaac
     7201 agtcgcggca gtgcctaaca atgctactct ggtgatgcag ttgtacaaca accgcctgtc
     7261 cgggcaattc acactgatcc ttcctcacta caatttcatt cctaccaact tatatcaaat
     7321 cactacttcc atgactacgg aacaaggata cgatacaggc tccggtgcgg tcacaaccgt
     7381 gcgtcaggct ttctcaaata cggtgcctta cactgtgacc tacacgacgc aatcgacaaa
     7441 caccacgcag gtggacttgc tcgactttgc ggccacgcag gatccgaact atctttccgg
     7501 gtccatgaaa ctgaagttca cacagccttc gaatgcggct gcggggcaga tcatagccac
     7561 atacggcttc aagttcgaca agctattcta aggaagtcat gatgagaacg ttatttgcac
     7621 tactcctcgc gctcactgct gtacagctca acgcggtaga accagaaaaa gatccgcccg
     7681 tagaaactcc taaaaagaaa ggaggagacc cgggcctcga aggattcaaa gtcacaggtc
     7741 tgcctctcat ttcattttcc acagatgatg gatttggata cggtgttcgc gcctacggca
     7801 catactacga agagggttat gctcccttca agttccaggc tttcgcacag tattacaaaa
     7861 cgactcgcgg ctatgaatac cacgaagtgt ctctcgatgc tttgaagttc ctgggtactc
     7921 ctctccgcct cagactggac ggcggttttg aaagaactct caatgcacaa tggtatggtt
     7981 atggaaacta tcatgatctg gcgcgcatca agcagatcaa gaatggcgaa atcccaatca
     8041 acgaaaacac tccgccaaac acgaaacgag atctgtatca gtatgatcag aacatcactc
     8101 tgaacaacct cgcactcaca aacccgtttg gttcaggcaa ccagatcttc aacccatcgc
     8161 agagagtttt gcgcgaaagt caggataagt atttctacta tgaccggatt cgtccgttca
     8221 tcaacatctc aacggaagat ttcattggag acacaaactt caagtggttc gccggtgtca
     8281 gagggcagcg ctacaaaatc cagtcctatc aaggcgacta tgacaacggc aacactgttg
     8341 caaacatcaa gacgctgatc gacaacgaac gcccatacgg ttatgatgcg actcaagaca
     8401 ggaaatacgt caacggtgtt cgcggagcca tcgcgtatga ctcacgtcca cgcgacagag
     8461 aaagcaatcc taatggcggt atcttcacgg acattcacgt ggagggagtg ggcaaaggaa
     8521 cgggaagcca ctactctttc actcgcacca ctgtgacctg gagacagtac tttgacgtcg
     8581 caccttcttt cttcaattcc tttgacaaag aactggtgat tggataccgc cttctgggac
     8641 aggaaacgtc aggagatgtt ccattcttcg aagccggtcg tatctacacc atgcgcgaaa
     8701 gtgcagaggg tctgggcgga aacggcggtg tgcgcggcta ttctgcgaac cagttcgtag
     8761 acaaagacat ggtcattgga aacctggaaa tgcgtctgac cacattcaaa atctcggcac
     8821 tcggtggtat ggactttgtt ctgctcggct accatgacga aggacgcgtg ggtgcctccc
     8881 gccgcgagat cgacatggac aactggcaca aggcaaacgg tggcggtgtt cgcattgtat
     8941 ggcagaaaaa tacaatcatc aacatcagct acggtcgctc caagtacgga gcaaacggca
     9001 acttctcctt caatcacctc ttctaagcga actgcttttt tgcgggcccc tgcgcccgca
     9061 aaaaaagcta gaaccttttc ccgccgtgac aaatcgaacc tatatgcgcc attttttgcc
     9121 cttgttcttt ctacttgcaa cagcggttgc cgcccaatct gaaaagggaa tcgacgtagt
     9181 acagagagcc aatggcgttt tcatttaccg ctacgatgag actctagaac agattgatcc
     9241 cagaaaagat ttcaaagcaa aaaaacaaat cacagagcaa agtgtaattg ctgatctaaa
     9301 aagactcctg aaagacaaca cagactacac gcccaatttc aaagcccggt gcatgcctgt
     9361 ttgggatgct ggaatcgagt ttcgcacgcc aactgaaacg cgaatgtatt tgttttcttt
     9421 cagatgcaac acggtcaaat ccgtagaaga caacactttc aaagacttca cgccgcaaag
     9481 aacggaatta tacggattac tcatgtacga aatcaatgat cggacgtcct acagtggccg
     9541 ttaggcgaca tttgcttcta ttgctggtcc tggcgacgcc gctctggtcg caggctccgc
     9601 ggcaaccagt catagcaatt cttccttttc aggcaaaaga tagcgccaga ggggaagagc
     9661 tcgcatcgcg cctcacggct gaaatcgtgg ccagacaggc tttccccgtc gcagagcggg
     9721 ctcagctgaa aaaagcattg gaagaacttg cgaagggaca gactggactt tttcaaccgg
     9781 agaatgctcc acagctgggt cgaatgattg gagctgacta cttgatcctg ggagaaatcc
     9841 tggattcaac agacaaaggc ggcgtgcgcg catcgatgcg ggtagtgcgc gcggaaacag
     9901 gaattgtaat tggagcagcc gtaacaggtg gagaagcaga cgctgtttcc agagaccttt
     9961 ctctatccgc gataaagaca gtttctatcc atgcgatgct ggaaaatccg gacagcccct
    10021 acagcgttct tctccttctg gacaagggaa aaaatccaga atacaaactg ggtgagacgc
    10081 ttcgcctgaa gttcaaggtt ctaaaacacg atcaaaccgc atctgaaaaa gtttatcttt
    10141 ctatatattc tataaacgca gacggtgtca tgactcttat ctatcccaat cgtttttcgg
    10201 gtcaggaagg ggtgtatgtt ggtcgggagt acacatttcc ggatccacgt gacgactttg
    10261 aatggaaact ggttccgccg gccggtgcag aaacgctcca ggcaatcgtt tcaaataagc
    10321 ctgtgagact tgcaaatgaa agagcaacgg aggactttcc cgttctaggt gtaaaagaat
    10381 caccggccct ctaccgcggt atccagaccc agctgaaaga agaaaaacgc ggaaactggg
    10441 ctgcagaaag aatcacgtac attctcaagc cgtagccttc agaatcgccc aggtcctccc
    10501 cgatattgtg tatgaatgtc tctgttgact ctagatctcc aattgccgcg caatgaatcg
    10561 cagaacatac ccagactcct gaatggatcg gcggggcagg tcctctcatg gaatctactc
    10621 tactcagatc ctcaaacctc ctactaccga attgaactat ttggaacatc tgcaccggaa
    10681 gaacttgagg cgcatatcct cgaaggctgc ccccaggcgc gcgtaaaaat caaagacgac
    10741 actgcccatg ggtcagacgc acttctcgcc atcgggccgg ccaggtccct tgaatttcgc
    10801 aaagcgttcc accgcacatc tcctgaagtt gtagagaatt ccgcgtcact gctcttgaaa
    10861 aacagagagg acgctcgcga acttcccatg gtaaaagaac gcgctttgtt catttcagat
    10921 ggcggcgctc ttcgtgtgaa aaaccaggca ctccttgctc ttgagcgaga tgggtatctc
    10981 gcatgcaagt tcagcggaat gcaggcaatt ccactacttc tggatgcgag aaacgaagaa
    11041 gaattcatca aatcagtcat ggcgcttgca ggaaatgtgt ccgtgatccg cctttcgatg
    11101 ctgcaaagaa ccatgtcacg ccagattgtg gagagaatca cagagcaggc atccatcccg
    11161 gtcattcacg cagaatattt agaatcctct gcaacgatag ccgccattgt atgcaatgca
    11221 cttcaatatc atagcatgac tctagacgaa aaagtcgttg cgattgtcgg gcttggaccg
    11281 tgcgctctgg gcctctcgcg tatcctatcc aggatgggag catccaagct ctacggcgtg
    11341 gacacggaat atgcactgaa ttcgagattt gagaaagacg gcggaacggc cacttccctc
    11401 gatttcgcgc tcgaacgatc tgacgtggtg gtcattagcc cgggaactca ggtattgatc
    11461 gacccaaaga gactgcgcga aaatcaactg gtcctttctt tcgctccgtc tgccattgat
    11521 atgagcgcac tgaaaccaga ggtttccaga tcagttcatc tttgcgaaga accgcacccc
    11581 atttacgcgc tccctggact tcttgcggga gtgcacgcag gtgcaaaagc ggacatcgaa
    11641 atttcaattc gaattgcgga agtgctcgtg ggtcgatgcg gagacaatcg attccttccc
    11701 atcccttcgg aatcactcgt gcgcgcggaa gcagaagctg tgagcctcac ctccagacga
    11761 taagaccggg cgccgcgaaa aaactaaagt atctaaacta aaatacgctt tcagcaatca
    11821 gcttcttcgt gtccagaggc ggaacacgat caaaatgagc aagagccatc tcagagcgat
    11881 ctgtgaagac accgtcgctt ccgaattgat tcatcaacca taattgccat ttcttgttga
    11941 tcgtatacga atgaaccagc cttcccgcgc gatgtgcagg acccgtatgc cacggatagc
    12001 taagataacc aaccgggccc atccctgcgt ccagagagcg agctttttcc acgagtgcag
    12061 gccagccatc gcgagccccc atttcctcat ccaccagata gactcgagga acggtcgctg
    12121 cgatgttctt gaggacctgc acgctttccg cggagaacga ctggaagatc acagcagctt
    12181 tgccgcaacc cactctcaca gggcgaatca cagactcatc aatgagggcg ggagtatctg
    12241 cgcaaacgcg gtcacggcca atccagcctc tcttcgtaag aagatccacc agatcctttt
    12301 ccactccagg atagcgttct ggcgatttgg tttctaaata tacgcctggc ctgtgatttc
    12361 caccctccgc gatcgcaaga agatcttcta cagtttgtat cttcaatccc ttgaaacgat
    12421 cattcgcgcg gtccgggttt gcggaattga accacgaacc gaggtccagt ttgagtagct
    12481 ccgcatatgt gaaacgatct atcgtgtcct tctgtcttcc cggaaacacc tctgcaacat
    12541 tacttgttcg ttctaaagta tcatcatgga gtagaaccag aattccgtct ttggtccgct
    12601 gcacatcccc ttccaggtaa tcagcaccca gatcacgggc aatcaggtaa gccgctctgg
    12661 tttcctcggg tgccagatag gacgcgcctc tgtgcgcaat cacggccggg tgcggaattc
    12721 ccagcgcatt tgcaatcatt cgtccggcct ttggcggact ctggattctc gcataaacaa
    12781 agaacccaag aatcaaaact tccggaatca gagtgatgaa caagatcagg cgaatcgtgc
    12841 gaagggtcat ggatcgaatc ttcagatgca gatccgcctg taactcactt ttctcgcagg
    12901 cgttacttct tctcgagtcc gcgaaggaac tgtattctcc gggttttttc cggagattgc
    12961 cattcgagcg aggaaaaaac ggcaagggaa tgtggcgcag tcgtcacggc cccggcaaaa
    13021 ggctccatga attcctgcca tggttctgtc tgctctttgt gaggtgggag aggttttcca
    13081 ttgattgaat ctcgcaccat ggaaggagtc acaggagagt tgtttcgcat cccgcgaata
    13141 tttcttatgg taaacgggcc gcctgccttc ttatcgcgaa tcactttacc aaagaattga
    13201 aattcaatcg tttgaagccc ggcggaatag tctccttcca cactcgaagt cgccaccggt
    13261 cctgatttcg aaaacaagtt tgcatcaaaa gcaaaatatc ctgccttgtg aatccataca
    13321 gggatgcgaa catacaaatg gccctggcgc actgattcgg tggccccttg cttgaattct
    13381 gcgacgttat gcggcgttga aaaccagtgc gcacgttgat tgtgacgctt accctgaaca
    13441 ttaaagtcag cctcaagaaa catatcgccc cagtcctgca cgcctggctt caccagaaat
    13501 tgaatgctct gatctggtct gttcccggca tcaatgcgag gcgcggcgga ggccttttgt
    13561 tttccgcggt cttccgtgta tacgcgagcc ttcaccgaat ctactttcaa cggctgtccc
    13621 agcgaagatt tgcagtgaag atagatctta aattcttttg ttccaacgga cacggcgctc
    13681 tgcggaatca gagtgcacgc agcactcaat ccagaaaccg gcacaggcgg acgatcagaa
    13741 ttataagggt cgagtagatc tacctgcgtt tgttccagcg gacgactgtt gggcgggtat
    13801 ttggcccatt ccagatatcg ttcaagctcc gttggttcgg agtacacctg aagactgaac
    13861 agcagaatca gcaggcacca cgcccgaatc atttcaacct tctacgacgt aaatgatgct
    13921 gctctgaatt ccaagtccgg cctgcgcaga aacaagcgca gtcttcgcgc ccggaacctg
    13981 cgatgggccc gcctgcccgg tcacctgaag agatgcctcc atgaggcgag caaggcccga
    14041 tgcgtagatg atgccgccgg agagagcgcc gccagatgca ttcaccggtt tgcccgaaga
    14101 gcgatcaaac gcaccgtcgt tc
//
LOCUS       DAOQJL010000025        13885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2133_length_13885_cov_4.985683, whole genome shotgun sequence.
ACCESSION   DAOQJL010000025 DAOQJL010000000
VERSION     DAOQJL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_13885_cov_4.985683"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLG78_02015"
     CDS             <1..1235
                     /locus_tag="PLG78_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21909.1"
                     /translation="KEETGATEEERAAANEEMVREAVELLVRGRLFNIYAEGRSTDAR
                     RLNKIKLGFMMLAIQAEKAYDFKLNLRIVPYGYFYDRINKFQSSVCIIFGRPFKLRSL
                     VELPANFLSLSAKEQLVYEKKLMLAGKEKLQSEIEKLIISIHDPKLVYLIDGITAVYV
                     LSPVKYMGPFENVREKYALCKMISETIQIAAKSEEGAVRVETLKLLLKDYQTSLAEAR
                     VADALVRREYTWTSLGYHLKGLVMGILYAPLSFYGYLANVPSRQAARFMRYYVIEVKK
                     RPMVDGDEQSILAAAAAGAIAYPIYYIVAYYLFRHFVMPWMNGFLSISTHLKDLAFLP
                     AEHPVFLSILGATGAVYLMARLWRFSLFHGRRFRQGVRFLLDAISEIFLRSKLQKLRE
                     KRSELIDAMDFLIGDYYH"
     gene            1247..1930
                     /locus_tag="PLG78_02020"
     CDS             1247..1930
                     /locus_tag="PLG78_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNE21910.1"
                     /translation="MKKINFSFAMPALVAFALLFTIAACDRPKNATEQKKTGPSIVFL
                     GDSLTAGLGLPDDKTYPALVEKKLEAEGLKYRVINGGRSGDTTAGGLARLPWYLRKEI
                     EMRVLVIELGNNDAMRGQSVSSIEKNLTEIIRKTRAYDPSIKILLCELRTFPNLGREY
                     GTTFAAVFPRVAKKEKVPLLPFILEGVGGHPDLNQADGIHPNESGSVIVTENVYRAIR
                     PHLPAPPAQ"
     gene            2571..2816
                     /locus_tag="PLG78_02025"
     CDS             2571..2816
                     /locus_tag="PLG78_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21911.1"
                     /translation="MDDLAAERIRNITASSWIEVRYAGARETTAALNIFSKFADQDLS
                     FTDAVSFAVMRQEGLRDAISFDSHFERAGFRIWKAGR"
     gene            complement(2839..3297)
                     /locus_tag="PLG78_02030"
     CDS             complement(2839..3297)
                     /locus_tag="PLG78_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21912.1"
                     /translation="MKPEINDLTLVCQAFHKNRVRFIVIGAHACAMHGHIRATADVDV
                     LIQDQDENIEKAISAIHELYPHLAEPLTVDDFRESIVIKILDEPELDVSLSAWSVTFD
                     QASKHTEVIVLDGIEIPFMGLADLIQSKSTERDQDKWDVSVLKEIQKKRK"
     gene            complement(3294..3575)
                     /locus_tag="PLG78_02035"
     CDS             complement(3294..3575)
                     /locus_tag="PLG78_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21913.1"
                     /translation="MLSRNRLDDIFISRQGLIFRLNLPARTSTYIDAVKIIGKKKDLS
                     PAERVRAHARLIESMNLLRPYPRVTGKILRFKTWRDLDEFNRTRAANRT"
     gene            3655..5010
                     /locus_tag="PLG78_02040"
     CDS             3655..5010
                     /locus_tag="PLG78_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNE21914.1"
                     /translation="MKKQKRFYLTEKEMPERWYNVQADMPNPLKPALHPGTKQPAGPQ
                     DFAPLFPMELIKQEVTTERWIQIPDPVRDIYTLWRPTPLVRAFELEKALGTPARIYYK
                     YEGVSPTGSHKPNTAVPQAYYNKMEGVEHIATETGAGQWGSSLAFACQHFGIKCTVFM
                     VKVSFEQKPYRKNLMEIFGAKVIASPSTQTDAGKKILANDPSCTGSLGIAISEAVEVA
                     AKDPGTRYSLGSVLNHVLLHQTIIGLEAEQQFLLAEDEPDILIGAFGGGSNFAGFTFP
                     FLRHKIAGKKMRVIASEPAACPTLTRGKFAYDFGDTVGLTPLVAMHTLGHNFVPSAIH
                     AGGLRYHGAAPLVSQLLVDNLIEAVAYKQNECFASAMLFAKNEGIVPAPEAGHVIHAA
                     VVEALKCKESGESKVIAFNLCGHGHFDMSAYQAYMAGGLKDFELSDKELQENLAALPG
                     V"
     gene            complement(5074..5454)
                     /locus_tag="PLG78_02045"
     CDS             complement(5074..5454)
                     /locus_tag="PLG78_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE21915.1"
                     /translation="MCLLYPVTIAWVVLLHFLEVTLFLNLMSAATGLLWGYLMFSLVH
                     RNFHIEGTWFLKTAFTRKWFRHVRRLHDIHHHKLTNEGRMPYNMGICFYWFDRLFGTY
                     RPALGRFNSRGLEKSRELYAHLYK"
     gene            5528..5686
                     /locus_tag="PLG78_02050"
     CDS             5528..5686
                     /locus_tag="PLG78_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21916.1"
                     /translation="MTRAIIKDVVMHMSFAQELHLFAVEESVDPVSEKFGGYNRRQKC
                     ECYLHAVQ"
     gene            complement(5763..5978)
                     /locus_tag="PLG78_02055"
     CDS             complement(5763..5978)
                     /locus_tag="PLG78_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21917.1"
                     /translation="MKRIMALTLALWSGGCGINHWYNHRVKHCQLEAAVLSVAACSGI
                     TDANQRSACSAIAGAILYTECFEHDND"
     gene            complement(5975..6721)
                     /locus_tag="PLG78_02060"
     CDS             complement(5975..6721)
                     /locus_tag="PLG78_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE21918.1"
                     /translation="MRILAALAIAFTLTSKIAAAEKPVAVVISVQGDRALIQNSQSFD
                     LEPSDLIRAGDEIQTGADGKVSVQSSTGVIFQIGSLSHVKITDMNQTSDSSVLIVDLK
                     TGNIATIVSKTSGSNSVRIQAPTAAASVRGTEFLVEADEEETSVSVGDGEVEVEDAGG
                     TKARVRPGGVIRARLREKLREERIDARRAERLKVFGEFRTKRDEKFRERVEKLRNTKE
                     RIRKRREFIRNLREARRDSLKEAREERKKR"
     gene            6861..8933
                     /locus_tag="PLG78_02065"
     CDS             6861..8933
                     /locus_tag="PLG78_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE21919.1"
                     /translation="MGIQSDVYRSFFDRSIEGMFLTTPAGRYILVNSRLAEIYGFSSS
                     EEMMDHFQNIKTQLYVDPSRRDDFVKAMNEAGTVLEFESQVRRKDGTIIWISENARQV
                     RDEHGQIVHYEGTVVDITDRKLLAGTMARQQAFYHQLFHNSPLAMILLDAERSVISCN
                     SAFERLFGYNASEIRSSSIRDKIIPDELILEAENYRAAILTGSSHTKETIRRHKDGRD
                     IPVNMQGFPVMLEGSVAGIYYLYEDITERKAYEETIMRQAFHDSLTGLPNRHLFNDRL
                     NRAVERSKRRESYHFAVVLMDLDRFKKVNDTLGHLVGDRLLVHVGKILHSCLRTVDTA
                     ARLGGDEFALIEEFRSRQDVLLVLDRIHALLHEPFDVGERTLQTSGSMGIVINTSEYS
                     SAEELMRDADIAMYRAKEHRKPYQFFSREMQRELMEIMEIETDLKNAIAGQQLFLYYQ
                     PIVSLARKRLEGFEALLRWMHPSRGMMQPDHFIPIAEDTGMILPIGSWVIQEACRQLR
                     VWTDANRLWDDLTISINVSIRQFTHIDLPGEIERSLSLYEIDPGRLRLEITESTLIQD
                     MDEILAVLRELKRLGVKLAIDDFGTGYSSLSYLRELPVDTLKIDRSFISGENRSPDSF
                     QIVKLVTSMARSLGMTVVAEGVEEVYQQDILRDLQCDNAQGFLYSRPLKGDGVAEWIL
                     ENGFLEQR"
     gene            complement(8940..9407)
                     /locus_tag="PLG78_02070"
     CDS             complement(8940..9407)
                     /locus_tag="PLG78_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE21920.1"
                     /translation="MQRLHPRGIKNGTITPFIWFDTDVQEVAKFYNGIFKNSKVLSTN
                     PMSATIVINDQKLHLFNGGPHYKLNEAFSLMVSCETQKEIDYYWDKLLAGGGQESRCG
                     WLKDKYGLSWQIIPDVLGQLIGDKDRQKADRAMQAMLKMAKLDLAALKAAHQG"
     gene            complement(9388..9885)
                     /locus_tag="PLG78_02075"
     CDS             complement(9388..9885)
                     /locus_tag="PLG78_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE21921.1"
                     /translation="MLGKRNLTITAKSDLEIVWTRAFNAPPEFVFEAFTTPALVQRWL
                     LGPDGWTMPVCEIDLRVGGSYRYVWSHAQQGTMGVGGVYREIKKPSRIVQTEKFDQAW
                     YPGEALQTLELKKEGSMTWMTATALFETKEARDAALNSNMEKGMVPSYDRLDEIAEQN
                     AKAAS"
     gene            complement(9839..10249)
                     /locus_tag="PLG78_02080"
     CDS             complement(9839..10249)
                     /locus_tag="PLG78_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE21922.1"
                     /translation="MSYIPNESDRLDATFAALADPTRRAILMRLAKGDVSVMALAKPF
                     AMSQPAISKHLKVLEQAGLISRSQDGQKRPRKIESSAFALADEWLDKFRKLWEYRYEQ
                     LDFLLEEMKKTRPTKRREKYAGKKKSDNNRQKRS"
     gene            complement(10344..10895)
                     /locus_tag="PLG78_02085"
     CDS             complement(10344..10895)
                     /locus_tag="PLG78_02085"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE21923.1"
                     /translation="MKIALILLSVAFVFIAGFLAYSGALSTVVVKEEKVGPFHIVYRE
                     VKGSYRQTGKVSMEIQSLLKENGIDNDRFFAVYLDDPRSVPEENLRSLVGHILPPGSR
                     EAKLPAEYKSAILPERLALVSVFPLKTQASIFLGMFKVYPAIFAQAAKSGYPDKKPSL
                     EIYEPSKGQTIYIYPFEYDTSGK"
     gene            complement(10912..11394)
                     /locus_tag="PLG78_02090"
     CDS             complement(10912..11394)
                     /locus_tag="PLG78_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21924.1"
                     /translation="MKPRKHQGRITLRRQLFLHVFYPEEFLKTHLESMCEDLSDLESE
                     GALQARDLIDLALNGLKIRIKHIARRMRPYHHALLTATVAFPLFAAFSAISFLFLPIN
                     LLPWILDFPFQDSLLMRAAIVLSLVSSILLIASERSPKNFLCLCINAPVTLFWTVLLF
                     "
     gene            complement(11398..11718)
                     /locus_tag="PLG78_02095"
     CDS             complement(11398..11718)
                     /locus_tag="PLG78_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE21925.1"
                     /translation="MPSTEKLSPAALEILLILAGQSMHGYAIMKTSRGEGGVRLGPGT
                     LYRWLDSLSQTGYIRIKKEDGRRNLYEITPSGRKVLRDELSHAQNLLARAKVAGIRTQ
                     GSGG"
     gene            11901..13238
                     /locus_tag="PLG78_02100"
     CDS             11901..13238
                     /locus_tag="PLG78_02100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE21926.1"
                     /translation="MARLGLLLLVLFTHCYTSEEPVRTVRPVHVDEEKIRSLSYDLEA
                     YIQYHIAKGVFYSMTVGVVRGDDLVYSRYINSVPSRLYSVGSITKVFTATAAMRLQEK
                     GIIDFDERLSDVFPGLHVEQEMYHSPAVTLRHLLSHSSGMPDLRYYVPPEMIHHPEID
                     FPISPQTAPAGVQFRYSNLGYQLLGEAFKKISHRQISDIIDQEVYAAAGMNDSRMLPG
                     STGAYGVMTNVTDLAKFASVFLRRGLRKDARFLHAQSVDAMLRNQIYAPPAEFQDYNG
                     IGWRVRTDGQGIVQMYHVGGANGVAAWLQIFPRSNVALFYLGDPPKYEEDTMGVLVGI
                     QARLGNLATEFAGETRPLFVFRQSVSPETELPRFAGEYREPASGEVASVLLQGGGIWF
                     QKGISPPYPVVPECRAVFDGGYGNVQHAFILDQKDGHVLGVATNNGYYTKVTR"
     gene            13266..>13885
                     /locus_tag="PLG78_02105"
     CDS             13266..>13885
                     /locus_tag="PLG78_02105"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE21927.1"
                     /translation="MGAIWYLATFVLMGTCYGAFFLQGPWVWAPVVLVFGIVPILETL
                     PFLSRDVNNPTDERTLGQSTIFSDLLNAAVIFQCGLIGMLLIEASRSQNLLQLSGLII
                     STGLACGVYGINVGHELGHRADSFSQRMARLSLATSLYAHFFVEHNLGHHRNVATELD
                     PASSRRGEWLYTFWIRSIVMGYISAWKISAKRTRDRGHAFISIHNT"
BASE COUNT         3347 a         3355 c         3551 g         3632 t
ORIGIN      
        1 ataaagagga gacaggtgct acagaagaag agcgtgcggc cgccaacgaa gagatggtgc
       61 gcgaagcagt agagcttcta gtgaggggcc gtctatttaa tatttacgcg gaaggaagat
      121 ccacagacgc cagacgactg aacaaaatca aacttggatt catgatgctg gccattcagg
      181 cagagaaagc atatgacttc aaattgaatc ttcgcattgt accgtacgga tatttttacg
      241 atcgcatcaa caagtttcag agttctgttt gcattatctt tggaagaccg ttcaagctca
      301 gaagtcttgt ggagcttccc gccaattttc tttcgctctc tgccaaagag cagctcgtct
      361 acgaaaagaa actgatgctt gcaggcaaag aaaagctgca gtctgaaatt gaaaaactca
      421 tcatatccat tcacgatcca aagctcgttt acctgataga tggaatcaca gcagtttatg
      481 ttttgagtcc ggttaagtac atgggcccgt ttgaaaatgt gcgagaaaaa tatgctctgt
      541 gcaaaatgat ttctgaaacc atccagatcg cggcgaaatc agaggaaggt gcagtgagag
      601 tggagactct caagttactc ttgaaagact accagacttc tcttgcggaa gccagggttg
      661 cagacgcact cgtaaggcga gagtacacct ggacttccct gggctaccac ctcaaaggtc
      721 tcgtgatggg gattctctat gcacctctgt cattctatgg ctatctggca aacgtacctt
      781 cgcgtcaggc ggcgcgattc atgcgatact atgtgatcga ggtgaagaaa agaccaatgg
      841 tggatggaga tgagcagtcc attctggcgg cggcggccgc aggcgccatt gcctatccca
      901 tatactatat agttgcttat tatttgtttc gccactttgt gatgccctgg atgaatgggt
      961 ttttgtccat ctccactcat ttgaaagatc tagcattttt gcccgcggag caccctgtat
     1021 ttctgtctat cctgggcgcg accggggctg tgtatttgat ggcccgactc tggagatttt
     1081 ctctgtttca cggaagaagg tttcgtcagg gagttcgctt tctcctcgac gctatttcgg
     1141 agatctttct tcgaagcaag ttgcaaaaat tgagagagaa acgttcagaa ctgattgatg
     1201 cgatggactt tttgatcggg gattattatc attgaatgcg agttatatga aaaagatcaa
     1261 cttcagtttc gcaatgccag cactggttgc tttcgcactg ctcttcacca ttgcggcgtg
     1321 tgatcgccca aagaatgcaa ctgaacagaa gaagacgggt ccatcgattg tatttctggg
     1381 ggatagcctg acagcaggcc tgggtttgcc ggatgacaaa acctatcctg cacttgtgga
     1441 aaagaagctc gaggctgaag gattgaaata cagagtcatc aacggcggac gctcaggcga
     1501 cacaactgcg ggtggtctgg ccagacttcc gtggtatttg cgaaaagaaa tagagatgcg
     1561 cgtacttgtc atcgagcttg gaaataacga cgcgatgcgc ggccaatccg tttcttctat
     1621 tgaaaagaat cttactgaaa tcattcgtaa aacgcgcgcc tatgatcctt ctatcaagat
     1681 tctactctgc gaattgcgca cgtttcccaa tctgggccgc gagtatggga ctacatttgc
     1741 cgccgttttt ccgcgcgttg cgaagaaaga gaaagtcccg cttcttccct tcatcctgga
     1801 gggagtgggc ggtcatccgg atttgaatca ggcggacgga attcacccca acgaatcggg
     1861 ttccgtcatt gtcactgaga atgtgtatcg cgcgatccgg ccgcacctgc ctgcgccgcc
     1921 tgcacaatga catcttgcaa ggccctgata taaacgcttt ttttgggggc acgcacaaca
     1981 aaaagagcaa gtgtgatcgc gggctgtttt ggctctgctc ctagttataa gtgctcccgt
     2041 atgttccgag aatgctcgta cagtttgtct ggcaggtgcc cgtgctcgag caaaggggac
     2101 cagttcctcc cgaataataa acgcgctcag tcccggtagt gcgacatgag ccaaagtttg
     2161 tcaacgcgct gcaacgtgtc gtggaatagg taccactttg tgcaatgcat gtggattggc
     2221 cgcttgatcc gtagtagttg tcgcaagtgc tgttgacgct gaacttgttg caacttcctg
     2281 tcaatgtaga tgaggaggaa gccagaagca atcccgccag aagcgtagtt gttgaactat
     2341 catcattctc tttcttctta cagttcgaga agatcattgt tccgatcagt gtgagcaaga
     2401 ggattgatgc gatcgggagt gaggatcttt gcatctgccg aatgtcaagt ttcggggggg
     2461 gggcaactat ttttcgggct tgtacgggga ccgcgattgt aatatttggc cgcgccggag
     2521 aaaggaagaa atcgccctgc ggcgatttct tctacataaa gggggcgcca ttggatgatc
     2581 ttgctgcaga acgaatccgc aatatcaccg cgtcttcctg gattgaggtg cgctacgccg
     2641 gtgccagaga gaccacggct gccttgaaca ttttttccaa attcgcagat caagatctga
     2701 gcttcacaga cgctgtgtcc ttcgcagtca tgaggcagga agggttgcga gacgccattt
     2761 cctttgattc acattttgaa agggctggat tcagaatctg gaaggcgggc cgctaacatg
     2821 aaggacctgg cgcgctagtc acttgcgttt cttttgtatt tcctttagca cagatacgtc
     2881 ccatttatcc tggtcccgtt cggttgactt gctctggatc aggtcggcca aacccatgaa
     2941 tgggatttct attccatcga gaacaatcac ctcggtgtgt ttgcttgctt gatcgaacgt
     3001 cacggaccac gcagacaaac tcacgtctag ctccggttca tccagaatct taatcacaat
     3061 gctttcgcga aaatcatcca cagtaagagg ttccgcaaga tgaggataga gctcgtggat
     3121 tgctgaaatg gccttctcaa tattttcgtc ctgatcctga atcaaaacat ctacgtccgc
     3181 agtggcacga atgtggccat gcatggcaca ggcatgcgct ccaattacaa tgaatcgcac
     3241 gcgattcttg tggaatgcct gacaaacgag agtcagatcg tttatttctg gcttcatgtg
     3301 cgattggccg cgcgcgttcg gttgaattca tctagatctc tccacgtctt gaaacgaagg
     3361 atctttccgg tgacgcgagg ataagggcgc aggaggttca tggactcaat gagtctggcg
     3421 tgcgcgcgca cgcgttcggc tggactgaga tccttctttt tgccaatgat cttcacggcg
     3481 tcgatgtagg ttgaagtccg ggcaggcagg ttcaagcgaa aaatcaggcc ctgcctcgaa
     3541 atgaagatat catcgagacg atttctcgat agcacggccg gaccatgaca ttgctcataa
     3601 tgcgaagcct ttgaaattgt ttgaccggtc tatggctatc ttttgcctgc gagcatgaag
     3661 aaacagaaac gcttttatct tactgaaaaa gaaatgccgg aacgctggta caacgttcag
     3721 gcggatatgc ccaatccact caagcctgct cttcatccgg gcaccaaaca accggccggt
     3781 cctcaggatt tcgctccctt gtttccaatg gaattgatca aacaggaagt aacgacggag
     3841 cgctggattc agattccaga cccagtgcgc gacatctata ctctctggag accaactccc
     3901 ctcgtgcgtg ctttcgaact ggaaaaggct cttggaactc cggcacgaat ctactacaaa
     3961 tacgaaggag tctctcctac tggttcgcat aaaccaaaca ctgcggttcc gcaagcctac
     4021 tacaataaga tggaaggtgt ggagcacatc gcgactgaaa caggtgcggg gcagtgggga
     4081 agttcacttg cattcgcctg ccagcatttt ggaatcaaat gcaccgtgtt catggtaaaa
     4141 gtttcttttg aacaaaagcc atatcgcaaa aatctcatgg aaatctttgg cgcaaaagta
     4201 atcgcgtctc cgtctacaca aacggatgca ggaaaaaaga ttcttgcgaa cgatccttcc
     4261 tgcactggct ctcttggaat tgcaatttca gaggccgtag aagtggcagc aaaagatcca
     4321 ggcacccgct actctcttgg aagcgtgctc aatcacgttc tgctgcacca gaccatcatt
     4381 ggtctggaag cagagcagca gttcttgctg gcagaagatg agccggacat tctcattgga
     4441 gcgttcggtg gaggatctaa cttcgctgga tttacatttc cattcttgcg ccacaagatt
     4501 gctggaaaga aaatgcgcgt catcgcatct gagccggctg cctgtccaac gctcactcgc
     4561 ggaaaattcg cgtacgattt tggagacact gtcggcctta ctccgctcgt tgccatgcac
     4621 actctgggtc ataattttgt tccaagtgcg attcatgcgg gcggtcttcg ctatcacggc
     4681 gcggctcctc ttgtaagcca gctccttgtg gacaatttga ttgaagcagt tgcttacaaa
     4741 caaaacgaat gctttgcctc tgcgatgctt tttgcaaaga acgagggaat tgttccggca
     4801 ccagaagcag gtcatgtgat tcatgccgct gttgtggaag cgttgaagtg caaggagtcc
     4861 ggagaatcaa aagtgattgc ctttaacctc tgcggtcatg ggcatttcga tatgtccgcc
     4921 taccaggcat acatggcagg cggactcaaa gactttgaac tttcagacaa ggagctgcaa
     4981 gaaaacctgg cagcattgcc aggcgtttaa tcgctcctgg cgcttcgttg ggcgcctgac
     5041 tgttgttagg tgaggcgagc catccggctc gtctcactta tatagatggg cgtagagttc
     5101 gcgtgatttt tccaggccgc gcgaattgaa tcgtcccaga gcaggcctgt aggttccaaa
     5161 gagcctgtca aaccagtaaa aacaaattcc catgttgtag ggcatacgac cttcgtttgt
     5221 caacttgtga tgatggatgt cgtgaagtct gcgaacatgg cgaaaccact ttcgcgtgaa
     5281 tgcggttttt aggaaccacg tgccttctat gtgaaaattt ctgtgcacca gactgaacat
     5341 gagataaccc cagagtagac cggtggcggc ggacatcaga ttgagaaaca gtgtgacttc
     5401 cagaaaatga agaagtacca cccaggcgat cgtgaccggg tacaagaggc acaagccatt
     5461 ctgcgcccag accaaagaaa gtgaaacgcc cgatgcctga atccttgtaa tcctgcgatc
     5521 tctgcgaatg acgagggcca taatcaaaga tgtggtgatg catatgagct ttgctcagga
     5581 acttcacctt ttcgctgtgg aggagagtgt ggatccagta tccgagaaat tcggcggcta
     5641 caaccgacgc cagaagtgtg aatgctatct tcatgctgtg caatgagtca gaaggtgacg
     5701 gattgtcaag ccaaaacatt aacatcgtta accagccaga ggccaggtaa ccttccgcaa
     5761 tttcagtcgt tatcgtgttc gaagcattca gtgtagagaa tcgctcctgc aatggctgag
     5821 caggcgctgc gctggttggc gtccgtgatg ccactgcaag ccgccacaga cagaacggct
     5881 gcttcgagct ggcagtgttt cactcgatgg ttgtaccagt gattgatccc acagcctccg
     5941 ctccataagg cgagggtaag tgccatgatt cgtttcatct ttttttcctt tcttcgcgcg
     6001 cttccttgag agagtctcta cgcgcttcgc gcagattgcg aatgaactcg cggcgtttcc
     6061 taattctttc tttagtatta cgaagtttct ccactcgctc gcggaatttc tcatcgcgct
     6121 tcgtgcggaa ttctccaaaa accttgagtc tctctgcgcg cctcgcgtcg attctctctt
     6181 cgcgcagctt ttctctcaat cgagcgcgga tgactccgcc tggcctgaca cgagcttttg
     6241 ttccgcccgc atcttctact tctacttccc cgtcacccac agatacactg gtttcttctt
     6301 catctgcttc caccaggaat tctgttccgc gcacgctggc cgcggcagtg ggtgcctgga
     6361 tgcgcaccga gttggatcct gaagttttac ttacgatcgt tgctatgttg ccggtcttaa
     6421 gatccacaat caatacgctg gaatcagaag tttgattcat gtctgtgatc ttgacgtggg
     6481 aaagagaacc gatttgaaat atgacgccgg tggaagattg tacagatact ttcccatccg
     6541 cgcccgtttg aatctcatct cctgcacgaa tgagatcaga gggttccaga tcaaaagact
     6601 gtgagttctg gatcagtgcg cgatctccct ggacgctgat gaccacagcg accggctttt
     6661 ctgcagcggc gattttggag gtcagtgtga aagcaattgc gagtgctgcc aggattctca
     6721 tgcaacgatt ttgtccaggt gcgagcacag gagtcaatcc agacttggaa ggtcggcgcg
     6781 cgttttttct gaatcgcacg gattttttcc aaaaaatcat ctccattcga gaatggcacg
     6841 ctatatgcat tgaatgagtc atgggcattc aatcagacgt atatcgaagt tttttcgatc
     6901 gctctattga aggaatgttt ctgaccactc ctgcgggcag atacatactc gtgaattcca
     6961 gactcgccga aatatacggc tttagctcta gcgaagagat gatggatcac ttccagaaca
     7021 tcaaaacgca gctgtatgtc gatccttcca ggcgtgatga cttcgtaaaa gcgatgaatg
     7081 aggcaggaac cgttctagaa tttgagtctc aagtgcggcg aaaagacgga acgatcatct
     7141 ggatttcaga aaatgcccgg caggtgcgcg acgagcatgg acagattgtt cattatgaag
     7201 gcacagtggt ggatatcacg gatcgtaaac tgctggcagg aaccatggcg cgccagcagg
     7261 ctttctatca tcaattgttt cacaattctc ctctggcgat gatccttctg gatgcagaga
     7321 gaagcgtgat ctcatgcaac tccgcctttg agcggctatt cggatacaac gcatctgaaa
     7381 ttcgttcttc ctcgattcgc gataagatca tcccggatga attgattctc gaagctgaga
     7441 actatagagc ggctatctta accggaagca gtcacacgaa agaaacgatt cgtcgccaca
     7501 aagacggccg tgatattcca gtgaacatgc aaggatttcc agtgatgctg gaaggctccg
     7561 tggctggaat ttattatctc tacgaagaca taacggaaag aaaagcatac gaagagacaa
     7621 tcatgcgtca ggcgtttcat gattcactca cagggcttcc caatcgccat ctattcaatg
     7681 atcgcttgaa cagagctgtg gagcgaagca agaggagaga gtcgtatcac ttcgcggtcg
     7741 ttctcatgga cctggatcgt ttcaaaaaag taaacgatac tctgggtcat ctagtgggtg
     7801 accggcttct ggttcacgtt ggaaagattc tacattcctg tcttcgcaca gtggacaccg
     7861 ccgcgcgcct gggtggagac gaatttgctt tgattgagga attcagatcg aggcaggatg
     7921 tacttcttgt gctcgatcgg atccacgcat tgctgcacga accgtttgat gtgggagaga
     7981 gaactcttca gacttcggga agtatgggca ttgtcatcaa tacttcggag tattccagtg
     8041 cggaagagtt gatgcgcgat gcggacattg ccatgtatcg cgcgaaggaa catcgaaaac
     8101 catatcaatt tttcagccgc gaaatgcaac gtgagttgat ggagatcatg gaaatcgaaa
     8161 ccgatctcaa gaacgcaatc gcagggcagc aactttttct gtactaccag cccatcgttt
     8221 ctctcgcgcg aaagagactc gaaggatttg aagcactcct tcgatggatg cacccttcgc
     8281 gcggtatgat gcagccggat catttcattc cgatcgcaga agacaccgga atgattctgc
     8341 caatcggtag ctgggtgatc caggaagctt gcaggcaact gcgtgtgtgg acggatgcaa
     8401 atcgtctctg ggatgatctg accattagca tcaatgtgtc cattcgtcag ttcacacaca
     8461 ttgatctgcc tggagaaatt gagcgaagtc tctctctgta cgaaattgac ccgggccggt
     8521 tgagacttga gattacggag agcaccctca ttcaggacat ggatgaaatt cttgctgttc
     8581 tcagagagct caaacgtctg ggagtcaagt tagcgataga cgactttggg accggttact
     8641 cttcgctcag ttatcttcgc gaacttcccg tggacactct gaagattgat cgctctttca
     8701 tcagtggaga aaacagaagc cctgattcat ttcaaatcgt gaagttagtc acttctatgg
     8761 ccagaagtct tgggatgacg gttgtcgcag aaggcgtgga agaagtctac cagcaagaca
     8821 tcttgcgcga ccttcaatgc gacaatgctc agggctttct ctattccaga ccgctcaagg
     8881 gagacggggt tgcagaatgg attctggaga atggcttcct ggaacaacgt taagcggcat
     8941 taaccctgat gagctgcctt cagagccgcc agatcgagct ttgccatctt gagcatggcc
     9001 tgcatggctc tgtctgcttt ttgtctatct ttgtctccga tcagctgtcc tagaacatct
     9061 ggaatgatct gccatgatag tccgtatttg tctttgagcc agccgcagcg actttcctgt
     9121 ccgccacctg ccaggagctt gtcccagtag taatcgattt ccttttgagt ctcgcagctt
     9181 accatcaaag aaaatgcctc gttgagtttg tagtgcgggc ccccattgaa cagatgcaac
     9241 ttctgatcgt tgatcacaat cgttgcggac attggatttg tgctcaatac tttagaattc
     9301 ttaaatatcc cattgtaaaa ttttgcaact tcctgtacgt cagtgtcaaa ccagataaac
     9361 ggtgtaattg ttccattttt tattcctcta ggatgcagcc tttgcattct gctctgcgat
     9421 ttcgtccagt ctatcgtaac tcggtaccat tcctttttcc atgtttgaat tcagagccgc
     9481 atcccgggct tcttttgttt caaagagtgc agtggcagtc atccatgtca tgcttccttc
     9541 tttcttgagc tctagagtct ggagggcttc tccaggatac caggcctgat cgaacttttc
     9601 agtttggacg attctggaag gcttcttgat ctcgcggtat acgcccccga ctcccattgt
     9661 gccctgttgc gcgtggctcc acacgtatct atagcttcct ccgacgcgaa ggtcgatttc
     9721 gcagacgggc atggtccaac cgtccggacc gagtaaccac cgttgtacga gcgccggcgt
     9781 tgtgaacgct tcaaaaacaa attcaggtgg cgcattgaat gcgcgcgtcc aaacgatttc
     9841 aagatcgctt ttggcggtta ttgtcagatt tctttttccc agcatatttt tctctcctct
     9901 tggttggtct ggttttcttc atttcttcca gcaagaagtc cagttgctcg tatctgtatt
     9961 cccagagttt cctgaactta tctaaccatt cgtctgcgag cgcaaaagca ctgctttcaa
    10021 tcttgcgtgg acgcttttgt ccatcctggc tgcgagaaat gaggcccgcc tgctcgagca
    10081 ctttgagatg cttcgatatg gccggctggc tcatcgcgaa gggtttggca agtgccatca
    10141 cagacacgtc cccttttgcg agtctcatta agatcgcgcg ccgggtagga tccgcaaggg
    10201 ctgcaaatgt agcgtcgagc ctgtctgatt cgttcggtat ataactcata agacatataa
    10261 ccaataagtt atatatacgt tcgcgtcaac agcttttttg tccgctgttt gtttttgcga
    10321 tttcggtgac tggttagccc ggtctacttt ccggacgtat cgtattcaaa tggatatata
    10381 tagattgtct gaccctttga aggctcatag atctcaagag aaggcttttt gtcaggataa
    10441 ccagatttgg ctgcctgcgc gaaaattgcc ggatatactt tgaacattcc gagaaaaatg
    10501 gaagcctgcg ttttcagtgg aaagactgaa acgagtgcaa gtctttcagg gaggatggcg
    10561 ctcttatatt ccgctggaag ttttgcttcc ctggaccccg ggggaagaat gtgccccacc
    10621 aggctgcgaa ggttttcctc tggcacggat ctcggatcgt ccaggtaaac tgcgaagaat
    10681 cgatcattgt ctattccgtt ttctttgagc aagctctgga tttccatgga aactttccct
    10741 gtttgcctgt agctgccttt gacttctcga tagacaatgt gaaacggccc gactttttct
    10801 tccttcacca ctaccgtgga cagagctccg gaataggcaa ggaagcccgc aatgaaaaca
    10861 aaagcgactg agagtagtat gagagcgatt ttcatgaatt ttctccgcga ttcaaaataa
    10921 gagaacggtc cagaatagag ttacaggggc gttgatacac aaacacagaa agttttttgg
    10981 cgaacgttcg ctcgcgatca agaggataga ggataccagg gaaagaacga tcgccgcgcg
    11041 catgaggagt gaatcctgaa agggaaagtc cagaatccag gggaggagat tgatcggaag
    11101 aaagagaaat gaaatggcag agaacgccgc aaatagcggg aaggcaaccg ttgcggtcaa
    11161 aagagcgtga tgataggggc gcatccggcg cgcaatatgc ttgattctga ttttcagccc
    11221 gttcagtgcc agatctatga ggtcacgtgc ctgcagcgct ccttctgact ctagatcaga
    11281 aagatcttcg cacatggatt ccagatgtgt tttgagaaat tcctctggat agaatacatg
    11341 caaaaacagc tgtctgcgta gcgttatgcg accttgatgc tttctcggct tcattttcta
    11401 tccgccactc ccctgggtgc gaatgcccgc taccttcgct cgcgccagta gattctgggc
    11461 atgagaaagc tcatcgcgta agaccttcct tcccgaaggt gtgatttcat atagatttct
    11521 gcgaccgtct tctttcttga tgcggatgta accggtttga gaaagagagt ccagccagcg
    11581 gtagagtgta cccggaccca ggcgaacgcc tccttctccg cgagaagtct tcatgattgc
    11641 atacccgtgc atggattgtc ccgcaaggat caacaggatt tccagagcag ctggagagag
    11701 tttttcggtg gaaggcatgt ccaggatgga tatatccacg atggatatat cgtcaaatat
    11761 tttttgcccc tcggggggcg gagccctgcc cgggttcaaa ggcatggaaa acggaggatc
    11821 tgacccagga aaaactggct ctcggccgcg ccattttggt ttgactctct ttttaacgga
    11881 gccagtcagg ctggacgtct atggcccggc tcggactact gttgctcgtt ctattcactc
    11941 attgctacac cagcgaggag ccggtgcgca cggttcgtcc tgtccatgtg gacgaggaga
    12001 aaatcaggtc tctttcctat gatctcgagg cgtacatcca gtatcacatt gcaaagggtg
    12061 tgttctatag catgactgta ggtgtcgtcc gtggtgatga cctggtctac agccgctaca
    12121 tcaattccgt gccatctcgt ctctattctg tgggatccat tacaaaggta ttcactgcga
    12181 cggctgccat gcgccttcag gaaaagggca tcattgattt tgatgaacgc ctgtcagacg
    12241 tgttccccgg gcttcacgtt gaacaagaga tgtatcattc tcccgcggtg acgcttcggc
    12301 atcttctgtc tcattcttcc ggaatgccag accttcgtta ctatgttcct cctgaaatga
    12361 ttcatcatcc agagattgat tttccaatct ctccacagac cgctcctgca ggagtgcaat
    12421 tccgatactc gaatctcggc tatcaactgt taggcgaagc atttaaaaag atatcacaca
    12481 gacaaatttc ggacatcatt gatcaggaag tatacgcggc agcaggaatg aacgattcgc
    12541 gcatgcttcc aggttccact ggagcttacg gtgtgatgac gaacgtgacg gatctggcca
    12601 aattcgcgtc cgtatttttg aggcgaggct tacggaaaga cgcgcggttc ttgcatgcgc
    12661 agagcgtgga cgccatgctt cgcaatcaga tctatgcgcc gccggcggag tttcaggact
    12721 acaacggaat cggatggcga gtccgtacgg atggtcaggg tattgtgcag atgtatcacg
    12781 tagggggcgc aaacggtgtg gctgcctggc ttcaaatctt cccgcgcagc aacgttgctc
    12841 ttttctatct gggagatcct cctaagtacg aagaagatac aatgggagtt cttgttggaa
    12901 ttcaggcaag gctgggtaat ctagccacgg agtttgcggg agaaactcgc ccgctatttg
    12961 tgttcaggca gtctgtttct ccggaaactg agctgccgcg ttttgcagga gagtacagag
    13021 agcctgcctc aggcgaagtg gcaagcgtgc ttttgcaggg cggaggaatc tggtttcaaa
    13081 aaggaatctc cccgccttat cctgttgtgc ctgaatgcag agctgtgttt gacggcgggt
    13141 atggcaacgt acagcatgct tttatcctgg atcagaaaga cggccacgta ctgggagtcg
    13201 ccacgaacaa tggttactac acgaaagtga cccgttaagc ggaatataaa actggagtta
    13261 gaaaaatggg cgcgatctgg tatcttgcaa cgtttgtttt gatgggtacc tgctatggcg
    13321 ccttttttct tcagggacca tgggtctggg cgcctgtggt cctggtcttt ggcattgtcc
    13381 cgattctcga aacattgccg tttctgtcgc gtgacgtaaa caatccgacc gacgaacgca
    13441 ctctgggtca aagcactatc ttttcagatt tgttaaacgc ggccgtcatc tttcaatgcg
    13501 gtctcatcgg catgctcttg attgaggcgt ccagaagtca gaaccttctg caattgagcg
    13561 ggctcatcat ttcaactgga cttgcatgcg gcgtttacgg aatcaatgta gggcatgagc
    13621 tgggtcatcg tgcggattct ttttcgcaaa gaatggcgcg cctttcgcta gccacctctc
    13681 tttacgcgca cttcttcgta gaacacaacc tcggacatca tcgaaacgtt gccacagaac
    13741 ttgatcctgc aagctccaga cgcggagaat ggctctatac tttctggatt cgttccattg
    13801 tcatgggtta tatatccgcg tggaagatat cagcgaagag aaccagagat cgcgggcatg
    13861 cgttcatatc tattcataac acaat
//
LOCUS       DAOQJL010000026        13810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2147_length_13810_cov_4.271828, whole genome shotgun sequence.
ACCESSION   DAOQJL010000026 DAOQJL010000000
VERSION     DAOQJL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_13810_cov_4.271828"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLG78_02110"
     CDS             <1..632
                     /locus_tag="PLG78_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE21928.1"
                     /translation="MGMRRKARATLCIAFVLASACSRYPAPTYMAMALLAPPAASKRL
                     FTTTTVTLGSIAGVAGADSICASDTARPTAAVYKALIVDSVGNRRACATSNCSGGISE
                     NISWVLRPNQAYVRADGSTPVMQTNSAGIFDFNLGNLTNSIDGTTNRFWTGLATDWTV
                     AAATHCSDWTSSSGVVNGERGFPNNVTAAAISSGAVACNAARAILCVEQ"
     gene            complement(667..1536)
                     /gene="truB"
                     /locus_tag="PLG78_02115"
     CDS             complement(667..1536)
                     /gene="truB"
                     /locus_tag="PLG78_02115"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE21929.1"
                     /translation="MIGGFVPAWKPPGMGSAEIVGRLKRTIHADKVGHTGTLDRFAEG
                     LMLLVVGRSTCLCDFFLHADKRYSAIFSFGKSTETHDPEGSVLEERGEDESISFVSQH
                     LDQIREWIENQIHTTEQTPPLYSALKKQGKRYSDHARAGVKELPPARKVTIYSASLTS
                     HSGYQVTADFSVSGGTYIRSIARDLGEEFQFPVHLAGLRRSAIGSFLLNEDCWRSGEE
                     VRIRPPHEVLTWPRVKASDAEVRSLLFGQTPRLQMPSGSQFAWIVGADGLPVAWAEVL
                     DYGLYRVRRIMKG"
     gene            complement(1533..1925)
                     /gene="rbfA"
                     /locus_tag="PLG78_02120"
     CDS             complement(1533..1925)
                     /gene="rbfA"
                     /locus_tag="PLG78_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNE21930.1"
                     /translation="MKEIRQKKLESSLLREMALAIQRRSRTGDGIGLVSVTRVELKTD
                     MSEVIFHVSPFGDPEDNRMTMQTLNRLAGQLQSQISRDLHLRSTPRFSFRVDNSIKEG
                     DRILEKLESTKPPSSVSQESGKDDEAEE"
     gene            complement(1947..4502)
                     /gene="infB"
                     /locus_tag="PLG78_02125"
     CDS             complement(1947..4502)
                     /gene="infB"
                     /locus_tag="PLG78_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004474904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNE21931.1"
                     /translation="MSDNKSQPAKKKITIKKPLVEGQKARDISDTFSNKAEPKKAESR
                     PQSQAPSTTSSQPSAPPQPRQPQERPASGNTPSSSSQSSPPRQPQGQDRPPRPQGGYQ
                     DRPPRPQGSGYQGNNPRPQGSGYQGNNPRPQGSGYQGNNPRPQGSGYQGNNPRPQGSG
                     YQGNNPRTGGPGGQRQGSGYQGNNPRSGGPGGQRQPVRGGTPFDALLETVRKAQTGGP
                     AAQPAARKGPPAPRRTGPVSPERRKREEAEQRIRKLYSQMEKKKQAQSGTTVPKQIEI
                     MESIQVGELAKKLNLKPGDVISRLMKMGEMVTINKVIDADTATLLAAEFGCEVKVVSL
                     YEETVIQAEADSESDHQPRPPVVTIMGHVDHGKTKLLDTIRKTNVVATEAGAITQHIG
                     AYQVTTASGKKITFLDTPGHEAFTAMRARGAAVTDLVVLVVAADDGVKEQTVEAITHA
                     QEAKVPIIVAVNKIDLPGANVDRVKQALSVHNLQSEDWGGQTIFCEISAKQNIGIEHL
                     LDMILLQADVMGLTANPSIRGVGRVVEARVDPGKGPVATVLIQNGTLREGDPYVVGVF
                     SGRVRAMFDSFGHRLTEAGPATPIEISGIDGVPEAGDPFTSVVSEKYGREIASKRQHF
                     KQISDAVTRVQPTLGDLKTWIQDHKEVKVIIKADVQGSVEAIRDGLLKLSTDDVKVRV
                     IFGATGAISESDVTLASASNALIVGFQVRATPRAAELAEKSLVEIKYYNIIYNVIDEV
                     KAAMEGLLEPDKVEEVSGRAEIRTVFKISRMGNIAGCMVVQGKVRKANKVRLIRDGVV
                     IYTGSLRSLRRVKDDVPEVAEGFECGIAIEGYNDIREKDIIESFEIKEIARKL"
     gene            complement(4503..5855)
                     /gene="nusA"
                     /locus_tag="PLG78_02130"
     CDS             complement(4503..5855)
                     /gene="nusA"
                     /locus_tag="PLG78_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNE21932.1"
                     /translation="MARATKKKTNQEPDIKAFFDLLTEIATEKGLSRDEVVEVFKGSL
                     ITAYQKKYGLDANLEVILDREKPEIAVICRRKVVKPVLNTAQEISPEEAAKIEPGIKE
                     GDILSVTENPFEFSRIGATNVRQILLQRLKELEREILYNEFKAKENELVNGYFLRWRD
                     KDLMYVDLGKIEGVMPRREQIPNEKFRPGDRVKAVIKLVELRREKTREPGPFVTLSRG
                     SGEFVKKLFEMEIPEIYDGVVEITGIARYSGYRTKILVKSNRMDVDPVGACVGVRGVR
                     IQSVVRELGNERIDIVNDNGSPADIIASAMSPAVVTEVRADANTREALVLVPDDQQSK
                     AIGNGGQNVRLASQLTGYKLTVKTLSQFNEEMASPEARARLEQLFSQAQPEPEEEYTP
                     LSELPDLSSRIQSLLEAAGIKSVEQLVEADIEDLGKILGPATARHVQKVVSEAVEVTE
                     "
     gene            complement(5858..6364)
                     /locus_tag="PLG78_02135"
     CDS             complement(5858..6364)
                     /locus_tag="PLG78_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21933.1"
                     /translation="MIGELATQALGPGLAVFSANMHPVKSGFHIQVELDGLSHPQGAV
                     SVGDCEEFSKRLSNLIDQAVERQEPGVPAGFTAENYTLEVSSAGAERELRLPQDLLRF
                     KEVPIKLRYEEDGKEDTAIVTSAGKGEDGLYLFLAFKGRRKQRKQKFKELRLEESKIR
                     KANLFLDF"
     gene            6538..7629
                     /locus_tag="PLG78_02140"
     CDS             6538..7629
                     /locus_tag="PLG78_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21934.1"
                     /translation="MHIRVAACILLTLAGCARFRVQELKPTRVFAVPLLSAFPHLAKG
                     SDEFTRPIQIAGITSSPAILGNRIFIPDPTAGMVRIFKRNADTADSVVADKLEKGSEA
                     TLIRVRAGIPGRAVSDDDENVFIVLHEGPAPDEKNEPEPPPERRTTDRFNANAFMDTP
                     ARIVHIKEDQLAGIFGREGGKEGPAFERILRLHAIEGALYVFHSVGGARQLSVYQNEK
                     RIKQFENFTIPENDALKYFTDIEDMFPAGQSVIVSVVLRNKNTFAPEARRVYSWDGHT
                     ANLIYTIDEDADQFIAARPDGGFTVANSEDGNRMLLKIYSPSGEYLNNRLIVMPDFRN
                     TWREVTVDAAGRVFSNRIYKGSFEVYEWK"
     gene            7616..9076
                     /locus_tag="PLG78_02145"
     CDS             7616..9076
                     /locus_tag="PLG78_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNE21935.1"
                     /translation="MNGNRPLLTYAQSRTLDLDTIAAGFTEQQLMGQAAVASVNCLLS
                     DGTLDSHETILILCGKGNNGGDGYAIAWALIGAGFSSRVQIFAAEPPSSQSALFYRRM
                     CEKHTPIQDLTHFAQKASGPGDLILECLLGSGQKGDLRPPYLESAMDIARACKRGATL
                     IAVDCPVGLIEERTASFGEELPLPDEVHCYGPIKLAAALNVSLATGSLVRSLPIGFEP
                     VESSAWLWDGPPVQFHKSSIDHKYSAGSAAILGGEPGMEGAAILASDSFFAAGGGICE
                     VLTTSAESRSRILSARPSLMVRTLEEGIHVRAKAILAGPGILLDDQKAAQILRILSSV
                     PTDTRVILDASTAELVRDSRYPALLKPGTMLTPHGGEWKKMGGQLPDCVQAFEESQAF
                     ARTLGVSVLVKGPVSVLFSAKTWIHSHPNPSLAVAGSGDCLGGIILSSLSRGHEMAAG
                     VLSSMDLLHEAVSGLTHPEAHEFAENIRRILSVTRA"
     gene            complement(9120..9845)
                     /locus_tag="PLG78_02150"
     CDS             complement(9120..9845)
                     /locus_tag="PLG78_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21936.1"
                     /translation="MTEAKRQLLPDENWFWKIVYAVSRTGFFIFVSACLPGAYLYIRG
                     QSAPVWLALPVAIGGCIWTLIYLGLHSYAFPTHGRGALLAFVVPVWNCVASGQATESF
                     LFGAYSVVLAAVVAQCLFLLPVSLAACISPLRRRIFGEDSALWIAPFLSVVFLLIAAF
                     GWTFRAPFLEQMQSTRSFVAMTGFAFQVVMEIRVLYFGSIFSSSRGEDSESSALHEAW
                     APLMIFCMIGSLAGAGILALWRS"
     gene            complement(9870..11495)
                     /locus_tag="PLG78_02155"
     CDS             complement(9870..11495)
                     /locus_tag="PLG78_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE21937.1"
                     /translation="MKGLMMDYNLTVDRILLRAEKMFPEQEIVSRLANGSIHRYKYGE
                     FFSRTIKLMNALRALGVKPGDRVATFAWNSYRHMEIYFAVPALGAVLHTLNIRLFPEQ
                     LVYIVNHAKDKLIFVDKSLSGMLAAQEPHFKGVQNYIVMDDLHAEPAASLPNTLDYEA
                     ILSAGKDTIEFPTIEEGTAAGMCYTSGTTGEPKGVLYSHRSIFLHAMGISMTDSLAIS
                     NRDVILPVVPMFHVMSWGIPFACCLTGAKPVYPGAQLIGKPIAELLEKERVTIAAGVP
                     TIWNVLLQHLQKETHDLSSLKTMVVGGSAAPRSMIEAYEKKFGVSILHAWGMTETSPL
                     GTVSRLKKHMDTWSEDEKYKVRAKQGMAVPLVEVKIVDDENKDLPWDGSSSGELLVRG
                     PWIASSYYNAETPDGNAAFTKDGWFRTGDVATIDSNGFMEITDRKKDLIKTRGEWISS
                     VEMENIIMSYAGVLEAAVVGRPDEVRGEAPVAFVVATAEKKGTLDAKGIHDLLSEKFA
                     HWQIPKLSDIRFIDAVPKTSVGKFDKKVLRKQV"
     gene            complement(11541..11852)
                     /locus_tag="PLG78_02160"
     CDS             complement(11541..11852)
                     /locus_tag="PLG78_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21938.1"
                     /translation="MGFTPRQFKLVQAAAGSLESLGIAREEAIILISNSIQEELTALG
                     LTMEQVDAKSRTERFAFIRTLAARMEKTIHTRGVMLGQIRAAMEEFMEALRQSWQEET
                     R"
     gene            complement(11903..12448)
                     /locus_tag="PLG78_02165"
     CDS             complement(11903..12448)
                     /locus_tag="PLG78_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE21939.1"
                     /translation="MCAFFLPRAIWHMPVELRALEISMKSDLAKLANDPEIFVNVRDF
                     FPHPYSELNAEEFISRSSLNPAPLEYAILTDGAFAGVCGFIPGTDIHRLTAEVGYWIG
                     KPFRGKGIATSALRQLCDVAFSRGFVRLYAGVFETNQPSMRVLEKSGFTLESIRKKGV
                     IKQGKILDEHLFVLLRPDTSL"
     gene            12533..>13810
                     /gene="gatB"
                     /locus_tag="PLG78_02170"
     CDS             12533..>13810
                     /gene="gatB"
                     /locus_tag="PLG78_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNE21940.1"
                     /translation="MAWHVVIGLEVHVQLGTRTKIFARDGYGFGSPANSLAGPVTLGL
                     PGVLPVLNEEVLRLAVIAGLALECKIAEISKFDRKHYFYPDLPKGYQISQYDQPYAKD
                     GRVTFRKKGDESDTTIRIHRIHMEEDAGKLLHAVKNRSYVDLNRAGVPLIEIVSEPDL
                     RSSDDAYHYLQTLRNILRSAGVSECSMEQGSLRCDANVSVRPTEDAPFGTRVEIKNLN
                     SFKAVKAAIEYEMQRQEELIKDGGKVIQSTVLWDADNRVTRLMRTKEDAEDYRYFPDP
                     DLLPFRIDPAWVDGLKKTMPELPAAKRERYMKEYDLPEYDADVLTRDLDVARYYEKVV
                     SICKDGKKSSNWVKDEVLGITNKNDIPISAFACTPERLGRMIQLLNEGKITGRIAKQV
                     FEHVYEENLDPDKVIEKYGYKPVDVSSLPDIINK"
BASE COUNT         3256 a         3708 c         3421 g         3425 t
ORIGIN      
        1 cgatgggcat gcgaaggaaa gcacgcgcca ctctgtgcat tgcctttgtt cttgcgtcgg
       61 cgtgcagccg ttaccccgcg cccacgtaca tggcgatggc cttactcgca ccacccgcgg
      121 catcaaaacg actattcact actactactg tgactctcgg ttccatcgcc ggagtggcgg
      181 gtgcagattc gatttgtgca tctgatacag cacgtcctac ggccgcggtt tacaaagcct
      241 tgatcgtgga cagcgtgggc aatcgccggg cctgcgcgac ttcgaactgc tcaggcggaa
      301 tctcagaaaa tatttcctgg gttctcagac caaatcaggc ctacgttcga gcggacggca
      361 gcactcctgt catgcagacc aattctgcgg gcatatttga tttcaatttg ggaaacctca
      421 ccaattccat cgacggaacc acaaatcgat tctggaccgg actggcaact gattggactg
      481 tagcggcggc tacccactgc tcagactgga catcgagttc cggagtggtc aatggggaaa
      541 ggggattccc aaacaatgtc acggccgctg ccatttcctc tggagcagtc gcatgcaatg
      601 cagcgcgcgc cattctttgt gtggagcagt gatttcgcac tccgcgcccg gaaaaggaac
      661 ccggcctcag cctttcatga ttcgccgcac gcgatagagc ccgtagtcca ggacttctgc
      721 ccatgcaacc gggaggccat ctgcacccac gatccaggcg aactgacttc cggatggcat
      781 ctgtaacctg ggggtttgtc cgaacaacaa actgcgaacc tctgcgtcgc ttgctttcac
      841 gcgcggccag gtaagaactt catgaggagg gcgaatgcgc acttcttctc cggaacgcca
      901 gcaatcttca ttgagcaaaa aagaaccgat ggcacttctt ctgagtccgg ccaggtgcac
      961 aggaaattga aactcttctc ctagatcgcg cgcgatagaa cgaatgtatg tgccgccaga
     1021 aacgctgaag tctgctgtga cctggtagcc tgagtgcgaa gtgagggatg ctgaataaat
     1081 ggttactttt cgcgcaggtg gaagttcctt cacaccggcc cgagcatgat ccgaatatct
     1141 ttttccctgt ttcttcaaag cagaatacaa tggaggagtc tgttctgttg tgtggatttg
     1201 attttcaatc cattcgcgaa tctgatccag atgttgtgaa acaaaggaga tggattcatc
     1261 ttcgcctctt tcttcgagca cgcttccttc tggatcatgc gtctctgtgc tcttgccaaa
     1321 tgaaaagatg gccgaataac gtttgtccgc atgcaaaaag aaatcgcata aacacgtaga
     1381 gcgcccgacg acaagaagca tcagaccttc cgcaaaacga tcgagagttc cagtgtgacc
     1441 tactttgtct gcgtgaatgg ttcttttgag tctgcctacg atttcagcag aacccatccc
     1501 tggcggtttc caggctggaa caaaaccgcc gatcactctt ccgcctcgtc atccttgccg
     1561 gactcctgcg atacagaaga cggcggcttc gtactttcta atttttctaa aatgcgatcc
     1621 ccttccttga tgctattgtc tacgcgaaag gaaaagcgcg gagtagaacg aaggtgaaga
     1681 tcgcgactga tctgtgattg tagctggccc gccagacgat tgagagtctg catggtcatg
     1741 cgattgtctt ccggatcccc gaatggagaa acatgaaaga tgacttctga catgtctgtt
     1801 ttcaattcaa cgcgcgtaac agaaacaaga cctatcccat ctcccgtccg actgcgtctt
     1861 tgaatcgcaa gagccatctc gcgaagaagc gatgactcca gtttcttttg gcgaatttct
     1921 ttcatgaaag aatcgggccc gtttgcttag agcttacggg cgatttcttt gatctcgaac
     1981 gattcaatga tatccttttc acgaatgtca ttgtaacctt cgatggcaat cccgcattcg
     2041 aatccttccg caacttcggg cacgtcgtct ttgacgcgtc ttagactgcg aagagatcca
     2101 gtgtagatca ccacaccgtc gcgaatcagg cgcactttgt ttgctttgcg gactttgccc
     2161 tggacaacca tgcaacctgc aatgttaccc atacgcgaaa tcttgaagac tgtacgaatt
     2221 tccgcacggc cagaaacctc ttccactttg tccggctcaa gaaggccttc catcgcggct
     2281 ttcacttcat caattacgtt gtaaatgatg ttgtagtatt tgatttctac gaggctcttc
     2341 tctgcaagct ctgctgcgcg cggtgtagcg cgcacctgaa agccgacgat cagtgcattg
     2401 gaggcagaag ccagtgttac gtccgattcg gaaatcgcac cggttgcacc aaagatcacg
     2461 cgaactttca catcgtctgt ggaaagtttg aggagaccgt cgcgaattgc ttccacggaa
     2521 ccctgcacgt ctgctttgat gatgacttta acttccttgt gatcctgaat ccaggtcttg
     2581 agatctccca gagtaggctg cacccgagtg actgcgtctg aaatttgttt gaaatgctgg
     2641 cgtttagatg cgatctcgcg accatacttt tcgctcacaa cggatgtgaa cggatcacct
     2701 gcttctggaa caccgtcgat accactgatt tcaatcggag tggcaggacc ggcttccgta
     2761 agtctgtgac caaagctgtc gaacatcgca cgcacgcggc ctgagaaaac cccaaccacg
     2821 tacggatcgc cttcgcgcag tgttccattt tgaatgagaa ctgtagcgac cggacctttg
     2881 cccggatcga ctcttgcctc tactacacga cctacaccgc gaatagaagg attcgcagtc
     2941 agacccatga catctgcctg aagtagaatc atgtcgagca ggtgttcaat tccaatgttt
     3001 tgcttggctg agatttcgca gaagatcgtc tgaccgcccc agtcttctga ctgcagattg
     3061 tgcacactga gtgcctgctt cacgcgatct acatttgcgc cgggcaagtc gatcttgttg
     3121 acagcaacga tgatgggcac tttggcttct tgcgcgtgag tgatggcttc tacagtttgc
     3181 tcttttacgc catcatctgc tgcgacaacc agtacaacca ggtcagttac agccgcacca
     3241 cgagcacgca tggcagtgaa cgcttcgtga ccaggagtat ccaggaaagt aatcttctta
     3301 ccgcttgccg ttgtgacctg atacgcaccg atgtgctgcg tgatcgctcc ggcttctgtg
     3361 gccacgacgt tggtcttgcg aattgtatcg agtagttttg ttttaccatg gtctacgtga
     3421 cccatgattg taaccacagg aggacgaggc tggtgatctg attcgctgtc tgcttccgct
     3481 tgaattacag tttcttcata gagagaaaca acttttactt cgcatccgaa ttcagctgcc
     3541 agaagagttg cagtatccgc gtcgatgact ttgttgatgg tcaccatctc tcccattttc
     3601 atgaggcggc tgattacatc tccaggcttg aggttgagct tctttgcaag ctcacccacc
     3661 tggatcgatt ccatgatttc aatttgcttg ggcacagtcg taccggactg cgcctgtttc
     3721 tttttctcca tctggctgta gagtttgcga attctctgtt ctgcttcttc gcgctttcta
     3781 cgttctggag aaaccgggcc cgtgcgacgg ggagccggtg gaccttttct ggcagctggt
     3841 tgtgctgcag gtccgccagt ctgtgctttg cgtacagttt ctaaaagtgc atcaaatgga
     3901 gttccgccgc gtacaggctg cctttggccg cctggtccgc cagatctagg attgtttccc
     3961 tggtagcccg aaccctgtct ttggccgcct ggtccaccag tcctgggatt gtttccctga
     4021 tagcctgatc cttgcgggcg aggattgttt ccctggtaac cggatccttg cggacgagga
     4081 ttgtttccct gatagcctga tccttgcgga cgaggattgt ttccctggta gcctgatccc
     4141 tgcggacgag gattgtttcc ctggtaaccg gatccttgcg gacgaggtgg acgatcctga
     4201 tatcctccct gaggacgagg aggacgatcc tgtccctgag gttgtctggg cggcgaagat
     4261 tgcgatgagg aagatggagt gttccctgac gccgggcgtt cctgaggttg cctgggctga
     4321 ggtggcgcgg atggctggga agaagtggtg ctcggggcct ggctttgagg gcgcgattcc
     4381 gccttctttg gctccgcttt gttcgagaaa gtatcagaaa tatcacgagc cttctggcct
     4441 tctaccagtg gcttcttgat cgtgattttc ttcttggccg gctggctctt gttatctgac
     4501 atttactcgg ttacctctac tgcttctgac acaaccttct gcacgtgacg ggcagtggcc
     4561 ggaccaagaa tcttaccaag atcctcgata tctgcttcca ccagttgctc tacactctta
     4621 atacctgccg cttccagaag cgattggatg cgtgaggaaa gatcaggcag ctctgaaagg
     4681 ggggtgtatt cctcttctgg ctcgggctgt gcctggctga acaactgttc cagcctggct
     4741 ctggcctctg gagatgccat ctcttcgttg aactgagaaa gcgttttcac ggtcagctta
     4801 taacctgtaa gctgggatgc aagccttaca ttctgcccac cattgccgat cgccttgctt
     4861 tgctgatcgt ctggaacaag aaccagagct tcgcgggtgt tcgcatccgc tctcacttct
     4921 gtgaccacag caggagacat agcgcttgcg atgatgtcag cggggcttcc gttgtcgttt
     4981 acgatgtcga ttctctcgtt tccaagctct cgcaccacgg attgaatgcg aactccgcga
     5041 acgccaacgc atgcgcccac tggatccaca tccatacgat tggatttcac aagaatcttc
     5101 gtgcgatagc cggaatacct ggcgatgcca gtgatctcaa ctacgccgtc atagatttcc
     5161 gggatctcca tttcgaatag tttcttaaca aactcacctg aaccgcgaga aagagttaca
     5221 aacggacccg gctcacgcgt tttttcgcga cgcagttcta ccagtttgat gactgctttt
     5281 acacgatctc ctggacgaaa cttttcgttc gggatttgct cacgacgagg catgactccc
     5341 tcgattttgc cgaggtccac atacatcagg tccttatcgc gccagcgaag gaagtaacca
     5401 tttacgagtt cgttttcttt tgccttgaat tcattgtata agatttcgcg ttcgagctct
     5461 ttgaggcgct gtagtaagat ctgacgaaca ttggttgcac cgatgcgcga gaattcaaaa
     5521 ggattctcag tgacagatag aatgtctccc tctttgattc ctggttcgat ctttgccgct
     5581 tcttctggag aaatttcttg cgctgtgttc agtacaggtt tgactacctt gcgacggcag
     5641 atcaccgcga tttctggttt ttcgcgatcc agaatcactt cgaggttcgc gtccaggccg
     5701 tacttcttct gatacgctgt gatgagactg cctttgaata cttccaccac ttcgtcgcgc
     5761 gaaagtccct tctctgttgc aatctctgtg agaagatcaa agaatgcttt gatatcaggt
     5821 tcttgattcg ttttcttttt cgtagctctt gccatattca aaaatccaga aatagattcg
     5881 cttttcgaat cttcgactct tcgagtcgaa gttctttgaa cttctgtttt ctctgcttcc
     5941 ttctgccttt gaaggccaga aacaaataga gtccgtcttc ccccttcccg gcacttgtca
     6001 cgatcgccgt gtcctctttc ccatcttctt cgtaacgaag cttgatggga acttccttga
     6061 accgcagaag atcctgggga agcctgagct cgcgctctgc tccagccgag gaaacctcga
     6121 gcgtgtagtt ctccgcagtg aacccggctg gaacacctgg ttcctgccgc tccactgcct
     6181 gatcgattag attagacagc cgctttgaga actcttcaca gtcaccgaca gaaacggcac
     6241 cctgagggtg cgatagaccg tctagctcca cctggatgtg aaagccgctt ttgaccgggt
     6301 gcatgttcgc gctgaagacc gcaagccccg gcccaagcgc ctgggttgca agttccccga
     6361 tcatttttcc gcctgtgacc aaagaaaagt ggaaattccg cggaggcaga tcccactctt
     6421 caatcctgtc cagaaaacct gaacactggc ctggcgtcaa gtcaaaggct gcatgacttc
     6481 cagctcgcaa aaaggattca tccttgcctt ggttgcacga actccaaaag tcgcctcgtg
     6541 cacatccgtg ttgcagcctg tatccttctc acacttgccg ggtgcgcgcg attccgcgtt
     6601 caagaactca aaccaacgcg cgtattcgca gtgccactcc tatctgcatt tcctcatctg
     6661 gcaaaaggtt ctgacgaatt cactcgcccc atacaaattg cagggatcac atcgagcccg
     6721 gcgatcctcg gaaaccgcat cttcatcccg gatccaactg cgggcatggt gagaatattc
     6781 aaacgaaatg cagacactgc agattcggtc gtagcagaca agcttgagaa aggatcggaa
     6841 gccacactca ttcgagtgcg tgctggaatt ccagggcgcg ccgtttcaga cgatgatgaa
     6901 aacgtcttca ttgtgctgca cgaagggcct gctccggatg aaaagaacga gcccgaacct
     6961 ccgccagagc gcagaaccac agacagattc aacgcaaatg cttttatgga cacgccggca
     7021 aggatcgttc atatcaaaga agatcagctg gccgggattt ttggacgaga gggcggaaaa
     7081 gaaggtcctg cgtttgagcg aattcttcgc ctccacgcca ttgaaggtgc cctctatgtc
     7141 tttcattctg tggggggagc gcgacagctt tctgtttacc agaacgaaaa gagaatcaaa
     7201 cagtttgaaa actttactat cccggaaaac gatgcgctga aatactttac agatatagag
     7261 gatatgtttc ctgcgggcca atccgtaatc gtaagcgttg tgctcagaaa caaaaacaca
     7321 tttgcgccag aagccagaag agtgtattcc tgggatggcc acactgcaaa tctgatctac
     7381 acgattgatg aagacgcaga tcaattcatc gccgcgcggc cggacggagg tttcactgtt
     7441 gcaaactccg aagacggaaa tcgcatgctt ctcaaaatct attctccgtc cggtgaatat
     7501 ctaaacaaca gactcatcgt aatgcctgac ttcagaaaca cgtggcgcga agtgacagtg
     7561 gacgcagccg gcagagtttt ctccaatcgc atctacaaag gaagcttcga ggtctatgaa
     7621 tggaaatagg ccactcctta cctatgcgca atcgcgcact ctagatttag atacaatcgc
     7681 cgccggattc acagaacagc aactgatggg acaggccgca gtcgcgagcg tcaactgcct
     7741 gctttcggat ggaacgctgg attcgcacga aaccattttg attctctgtg gaaagggaaa
     7801 caacggtggc gacggttatg caattgcctg ggcattgata ggagctggat tctcatcgcg
     7861 ggttcagata tttgcagcag agccgccctc ttctcaatct gctcttttct atagaagaat
     7921 gtgcgaaaag catacgccga ttcaggatct aactcatttc gcacagaaag catccggccc
     7981 gggcgatttg attctggaat gcctgctcgg gtcaggacag aagggtgatc ttcgcccgcc
     8041 ttacctggaa tccgccatgg acattgcgcg cgcgtgcaag agaggagcca ctctcattgc
     8101 agtggactgc cccgttggac tcattgaaga aagaaccgct tctttcggag aggaacttcc
     8161 tctgccagac gaagttcatt gctacgggcc tatcaaactg gcagctgctc tcaacgtgtc
     8221 gcttgctact ggatctctgg ttcgcagtct gccgatcggg tttgagcctg tcgaatccag
     8281 tgcgtggctc tgggacggac cacccgttca atttcataaa tcatctatag atcacaagta
     8341 ttctgccggg agcgctgcga tccttggagg cgaaccggga atggagggcg ctgccattct
     8401 tgcttccgac tctttttttg cggcaggagg gggaatctgt gaagtgctca ctacttctgc
     8461 ggaatcccgg tcgcggattc tatccgccag gccttctctg atggttcgca cactcgaaga
     8521 aggaatccat gttcgcgcaa aggcaattct ggctggccct ggaattctcc tggacgatca
     8581 aaaagcagct caaattctgc gcatcctttc ctcagttcca acggacacaa gggtgattct
     8641 ggacgcatct acggcagagc ttgtgcgcga ttcccgttat ccggcattgc tcaaacctgg
     8701 aaccatgctt actccacacg gtggtgaatg gaaaaagatg ggcggtcagc ttccagattg
     8761 tgtccaggcc tttgaagaat cgcaagcttt cgcgcgaacg ctcggggttt ctgtgctcgt
     8821 caaaggcccg gtcagtgtct tgttttccgc caaaacatgg attcacagtc accccaatcc
     8881 ttctcttgct gttgcgggta gcggggattg cctcggaggg atcattcttt cttctctatc
     8941 gaggggccat gaaatggctg cgggcgttct ttcctctatg gatctgttgc acgaggcggt
     9001 ttctggtctc actcatccag aggcgcacga attcgcagaa aacattcgtc gcattctatc
     9061 tgttacgcgt gcgtagcatt cgcgttcaca gttgcgcacc catgagttgc ggccgccctt
     9121 cacgatctcc agagagccag tatgcccgca cctgccagag atccaatcat gcaaaaaatc
     9181 ataagcggcg cccacgcttc atgcagtgcg gacgattctg agtcttctcc tctcgaagaa
     9241 gaaaaaatac tgccgaagta cagcactcga atctccatca ctacctggaa agcaaagcca
     9301 gtcatggcta cgaaactgcg cgttgattgc atctgctcca aaaacggagc acgaaatgtc
     9361 caaccaaacg cagcgatcag tagaaagact acagacaaaa acggcgcgat ccagagggcg
     9421 ctatcttctc caaaaatacg acggcgaaga ggcgaaatgc aggcagccag ggaaacagga
     9481 agcaggaaca aacactgcgc tacaactgca gccaacacta cagagtaggc gccaaataga
     9541 aacgattctg tggcctgacc agacgcaaca cagttccata ctggaactac aaacgcaaga
     9601 agtgcccctc ggccatgagt gggaaatgcg tagctgtgca gcccaagata aatgagagtc
     9661 cagatacaac caccaatggc gactggaaga gcaagccaga cgggtgcaga ttggccgcga
     9721 atgtatagat aagccccggg aagacaggcg gatacgaata taaaaaaacc agtccgactg
     9781 actgcgtata caatcttcca gaaccagttt tcatccggaa gcaactgtct ttttgcttct
     9841 gtcactgtat catccagttc gcgcgcgcgt cacacttgct ttcgcaacac tttcttatcg
     9901 aacttcccaa cgctggtctt tggaaccgca tcaatgaaac gaatgtcgct taactttgga
     9961 atctgccagt gcgcgaactt ttcgctcaat agatcatgaa tgcctttcgc atcgagagtg
    10021 cctttctttt ccgccgtagc cacaacgaaa gcaaccggag cctcccctct gacctcgtct
    10081 ggtctgccta caacggcagc ctcaagcact cctgcatacg acatgatgat gttttccatt
    10141 tcaacgctcg aaatccactc tcccctggtc ttgatgaggt ctttcttacg atccgtgatt
    10201 tccataaatc cgttggaatc aatggtggcc acgtctcccg tgcgaaacca tccatccttc
    10261 gtgaacgcgg cgtttccatc cggagtctct gcattgtaat aggaagaagc aatccagggt
    10321 ccgcgcacca ggagctcgcc agaactcgag ccgtcccagg ggagatcttt gttttcatcg
    10381 tctacaattt tcacttccac gagaggaacg gccatcccct gcttggcgcg aaccttgtat
    10441 ttctcgtctt cgctccacgt gtccatgtgc ttcttcagtc tgctcactgt tccaagcgga
    10501 gacgtctctg tcatacccca cgcatgtaga atagaaactc caaacttctt ctcatatgct
    10561 tcaatcatgg aacgcggcgc ggctgatccg cccaccacca tggtcttaag cgaagacaga
    10621 tcatgtgtct ctttctgcaa atgttgaagc aatacattcc agattgtggg aactccggcg
    10681 gcaatggtaa cacgttcttt ttccagtagc tctgcaattg gctttccgat cagctgcgct
    10741 cctggataca ctggctttgc gcctgtgagg caacaggcga aagggatgcc ccagctcatc
    10801 acatgaaaca tgggcactac aggcagaatc acatctcgat tggaaatcgc aagagagtcc
    10861 gtcatgctga ttcccattgc atgcagaaaa atagaacgat gactgtagag gactcctttg
    10921 ggctctcctg tggtgccgga agtgtaacac attcccgcgg ccgtgccttc ttctatagtt
    10981 ggaaattcta tagtatcctt acccgcactt aaaatggcct cgtagtccag agtgttcgga
    11041 agcgaagcgg ccggttccgc atggagatcg tccatgacaa tgtagttctg aactcctttg
    11101 aagtgaggtt cctgcgctgc cagcattcca gagagagatt tatccacgaa gatgagcttg
    11161 tctttggcat ggttgacaat gtatacaagc tgctccggaa aaagacgaat gttcagagtg
    11221 tgaaggacag ctcccagggc cggcactgca aaatagattt ccatgtggcg ataactgttc
    11281 catgcgaaag tggccacgcg atctcctggc tttactccca gagcgcgaag agcattcatc
    11341 agttttatgg tgcgtgagaa gaattcgcca tatttgtatc tgtgaatgga tccatttgcc
    11401 aggcgactta cgatttcttg ttccgggaac atcttctctg ctcgcaggag aatcctgtcc
    11461 acagtgagat tgtagtccat catgagccct ttcatgggct gagtttccga cctcgggcga
    11521 cgggggtcaa tccacaattg tcagcgcgtt tcttcctgcc aggattgcct gagcgcttcc
    11581 atgaattctt ccatggctgc acggatttgt ccgagcatca ctccgcgcgt gtggattgtc
    11641 ttttccattc tcgccgcaag tgtacggatg aatgcaaatc tttccgttct gcttttcgca
    11701 tccacctgct ccatggtcag accaagtgcg gtgagctctt cttgaatgga attggaaatc
    11761 agaatgatgg cctcttcgcg cgcaattccc agactttcca gactgcctgc cgccgcctga
    11821 acgagtttga attgtcttgg agtgaacccc atgaagtgat catcgactgc tcagagcagg
    11881 gatcaataaa aaaatgcaac gatcaaagag acgtatcagg gcgcagtaga acgaacaggt
    11941 gctcgtccag gatctttccc tgcttgatga cgcccttctt tcgaatcgat tcaagagtaa
    12001 aacctgactt ttcgagcact cgcatggatg gctgattggt ttcaaatact cctgcataca
    12061 agcggacaaa gcctcgggaa aatgctacat cgcaaagctg tcgaagcgca gaagttgcga
    12121 tccccttccc gcgaaatggc ttcccgatcc agtaccccac ttccgcggtc agacggtgga
    12181 tgtctgtgcc tggaatgaaa ccgcatactc cggcaaacgc accgtctgtg aggatcgcat
    12241 actccagagg tgcgggattc agggaagacc tcgaaataaa ttcctccgcg tttagttcgc
    12301 tgtacggatg cggaaagaaa tcccgtacat ttacaaatat ctcgggatca ttggccagct
    12361 ttgccagatc cgacttcatg gaaatttcta gagcgcgcaa ctctacaggc atgtgccaga
    12421 tcgctcgggg gagaaaaaaa gcgcacatac ttttgcagac gccgtcaggc taatcggcac
    12481 aaaataccca gacaattact tgcgccccaa agtcgtcggg ctcagttgac tcatggcctg
    12541 gcatgtagtc atcggattgg aagttcacgt tcaactggga actcgcacta agattttcgc
    12601 gcgtgacgga tacggatttg gttcgcctgc aaactctctg gcgggaccgg tcacacttgg
    12661 attgcccgga gttctacccg ttctcaatga ggaagtgctt cgcctcgcgg tcattgctgg
    12721 cctcgcactc gagtgcaaga ttgcagaaat ttccaagttt gatcgcaagc attactttta
    12781 tccagatctt ccaaaaggtt accagatatc tcagtacgat cagccatacg ccaaagacgg
    12841 acgtgtaacg tttcgcaaga aaggagacga gtccgatacc acgatccgca tccatcgaat
    12901 tcatatggaa gaagatgcag gaaaattact ccacgctgtg aagaatcgtt cctatgtaga
    12961 cctcaaccgc gcgggtgtac ctctcattga aattgtatct gagccggacc tgaggagctc
    13021 ggatgatgcc taccactatc tgcagactct tcgcaacatt cttcgcagtg ccggtgtgtc
    13081 ggaatgcagt atggaacagg gaagcctccg ctgcgacgcg aacgtttccg tccggccaac
    13141 ggaagatgcc ccgtttggaa cgcgcgtaga aatcaagaac cttaactcat ttaaggcagt
    13201 gaaagcagcc atcgaatacg aaatgcagcg ccaggaggaa ctgatcaaag acggcggcaa
    13261 ggtcatccag tccacggttc tctgggatgc agacaatcgt gtaacacgcc ttatgcgaac
    13321 caaagaagac gcagaggatt atcgctattt cccagacccg gacctgcttc catttcgcat
    13381 tgatcctgcc tgggtagacg gactaaaaaa aacaatgcca gagctaccgg cggccaagcg
    13441 cgaaagatac atgaaagaat acgacctgcc cgaatacgac gcagacgttc tcactcgcga
    13501 cctggacgtt gcccgttact acgaaaaagt agtctctatc tgcaaagacg gaaagaagtc
    13561 ttcgaactgg gtcaaagacg aagttctggg aatcacaaac aagaatgaca tcccaatctc
    13621 ggctttcgca tgcactccgg aacgcctggg tcggatgatc cagcttctaa acgaaggcaa
    13681 gatcacagga cgaatcgcca agcaagtatt tgaacatgtc tacgaagaga acctggatcc
    13741 agacaaagtc atcgaaaaat atggctacaa gccagtggac gtttcttctc tgccggatat
    13801 catcaacaag
//
LOCUS       DAOQJL010000027        13713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2166_length_13713_cov_4.873920, whole genome shotgun sequence.
ACCESSION   DAOQJL010000027 DAOQJL010000000
VERSION     DAOQJL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_13713_cov_4.873920"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLG78_02175"
     CDS             <1..713
                     /locus_tag="PLG78_02175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE21941.1"
                     /translation="ARKIQASLLPRFVPDDDKCRISVLFSPAASVGGDLYEFHHLPNH
                     SVSFFVGDVSGHGVPAAMLSSLVKAAVSIQFTEGRLSPPIFLSSINEFLCRQLRGNFV
                     TAFYGHIDLKAMKLAYATAGHPHAYLWNPDRTTVTRIRTRGKMLGLFADLVFEEEIVP
                     LQPGDRILIPTDGILECENQAGRLFEEEGIERVISAHAAAPDLIVRLETALRAWTGLR
                     PGDDQTALLVDLKQTLQE"
     gene            753..826
                     /locus_tag="PLG78_02180"
     tRNA            753..826
                     /locus_tag="PLG78_02180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Asp,seq:gtc)
     gene            1173..2252
                     /locus_tag="PLG78_02185"
     CDS             1173..2252
                     /locus_tag="PLG78_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21942.1"
                     /translation="MAHQTLYSSHASAPVIQQTIIDRYAELFLHNPADYIKHNGNNWF
                     TVKGNATPAVLAAHLRHEIVIGLSPLKDGTIRWLALDFDAHNGESYSEISADLLAIVA
                     ALRRAGIQCHPEASGRGFHVWIFFAVPVRPNFIPALKQFVGNRCEVYAGKTRIRIPMG
                     VYQKDRSRFCGFLDHSLTEIPDQFEYLKNIKPTKPDQIVALNHLPPQGPAAVKTKITV
                     HSWYRNKPATATPKKDGRQVDRENLLFHFEIQPFINNRFSSVPNLKHRLVLYLGFALQ
                     LTAKETVNLRVRDLSENGSELKVAAPRCKAKTYLITDWAASIFRGLSEGKAPNVLVFQ
                     EGRKNFRERSVQKILQRFFESTLRG"
     gene            complement(2254..2667)
                     /locus_tag="PLG78_02190"
     CDS             complement(2254..2667)
                     /locus_tag="PLG78_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE21943.1"
                     /translation="MIRFFDTSALALVALRQNGWELAAQLYESASEVIVAFHTRVEFY
                     SALNRLQTDHAIGVTDAEQLYDWFDLESQRMRVIAFSDPVEQRALSILKQHQMKTLDC
                     LQLACALESGSTLERFVSSDKRLARIAEKFGLSSG"
     gene            complement(2664..2885)
                     /locus_tag="PLG78_02195"
     CDS             complement(2664..2885)
                     /locus_tag="PLG78_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21944.1"
                     /translation="MMQSELARVQEQLLEDGRNWSQHALQFGQFLAASTTSLDMNVSD
                     RARYTLPPKVNLLKPVDIVKIIEDDRYNA"
     gene            complement(2872..3270)
                     /locus_tag="PLG78_02200"
     CDS             complement(2872..3270)
                     /locus_tag="PLG78_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21945.1"
                     /translation="MAKPSSCFHFVPGHMAYEEDKEVDWSGLVAVGSLLGNLLQYTEK
                     NNLEKTLHWTGERLRAIAEDRERVIAMLHQVQKAYAVLKADADRMAATNKDLLTKLTE
                     SESEKGRLRNEVTELKEAIGILEKRLNDAK"
     gene            3367..5763
                     /locus_tag="PLG78_02205"
     CDS             3367..5763
                     /locus_tag="PLG78_02205"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNE21946.1"
                     /translation="MPIKKSELYSSIWQSCDELRGGMDASQYKDYVLVLLFIKYISDK
                     YSGGAFEPIKIPKGASFKDMVALKGKSDIGDKINTQIIGPLGKANNLSDMPDFNSEKL
                     GSGKEKVERLGNLISIFQNPALDFSGNRAEGDDLLGDAYEYLMRHFATESGKSKGQFY
                     TPAEVSRVIAQVIGIGEAKTTASTTVYDPTCGSGSLLLKVADEARTKVTLYGQEKDVA
                     TSSLARMNMILHDNPEALIENGNTLADPKFKEKEALKAFDYVVANPPFSDKRWSTGVD
                     TEKDPFGRFETFGVPPGKQGDYAYLLHIVRSLKKNGKGACILPHGVLFRGNAEAEIRK
                     NLVKHGYVKGIIGLPANLFYGTGIPACIIVLDKEEAPARKGIFMIDASAGYLKDGNKN
                     RLRARDIHKIVDVFKNLQEVPKYARMVSLAEIEKNEFNLNLPRYIDSQEAEDLQDIEG
                     HLRGGIPARDVDALSSYWKICPGLRKTLFKENRPGYVDLSVEKSAIKSAIFDHPEFSA
                     FIQKMNSHFQTWKEKNTAHLKKLKSGFHPKELIASLSEELLSHYEGQPLIDPYSVYQH
                     LMDYWSETMQDDGYMISADGWTAQTQRVLVKDKSGKEKDKGWVCDLIPKELVVHRFFA
                     KERETISTMESEAEGLAAQIQELEEEQTGEDGAFSELEKVNRASVAARLKEIAGEADS
                     KEEIQALETWTKLNTQEAELRRKIKELETELDDLAYKKYPKLTESEVKTLVVEDKWMA
                     ALDAQVHGEMDRISQTLTQRVKELAERYTTTLPEITKTVADLEKKVSAHLAKMGFAWN
                     "
     gene            5754..6932
                     /locus_tag="PLG78_02210"
     CDS             5754..6932
                     /locus_tag="PLG78_02210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNE21947.1"
                     /translation="MELKPRYKHTEVGVIPEEWELVKLRSLCPKSAYGPRFSSTLYSA
                     HGNVGTLRTTDMDDSGKISYETIPFAHLDENLFKAHFLEDGAFVVTRSGTCGIAAIFH
                     KIERPVVAGAFLIRFQFNDTVHRSFLKDMLNSTRGRRLTDSLIAGGVQKNLSGTGFLN
                     LKIPLPPFPEQKAIAEALGDVDALIESLEQLIAKKKQIKQGAMQELLTGKRRLPGFSG
                     EWEEKKLGDVCEITTGRKDVNQGNPNGIFPFFTCSRVHTFSDSYSFDTEAILIAGNGD
                     VGNLNYFAGKFEAYQRTYVLREFSVPVSYLWHHLSAFLAPALGLDKVGSSIPYIKREN
                     LIGFTYLSPRLAAESSAIATVLSEMDSEISAVEQKLSKTRAVKQGMMQELLTGRIRLV
                     "
     gene            7318..8244
                     /locus_tag="PLG78_02215"
     CDS             7318..8244
                     /locus_tag="PLG78_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNE21948.1"
                     /translation="MKNYYRVMAGKGSSLAEECFQQGFVGVDYGVAVDLTGKFPDDWR
                     QFNREFIPVYLQSHPEKTRIAAGSACGALWTLGRGMQKGDVVFVPDGEGSYHVGEVTS
                     DYQYQAGKNLPHRRQVRWYPEMVRRDATSPQMQGSTRSTGTIVDITGYANELEKLLSG
                     KGPAVLVSTDETVENPSAFALEKHLEDFLVANWKQTELGKNYDIYEEDGELTGQQYQT
                     DTGPIDILAISKDKTDLLVVELKKGRASDVVVGQTLRYMGYVQDELREPNQKVSGIII
                     ALEDDPKLKRALAMTPAIQFFRYEVSFKLRKA"
     gene            8246..11299
                     /locus_tag="PLG78_02220"
     CDS             8246..11299
                     /locus_tag="PLG78_02220"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNE21949.1"
                     /translation="MTSLNTKERATQNRIIQLFKNSLGYAYLGNWEEREGNSNIEEEH
                     LARWLAKRGFQKEHISSAIYKLKTEAHNPNRPLVHNNMAVYSMLVYGVDVKLDATKNT
                     EQVPLIDWENPDQNDFAIAEEVSLHGAAERRPDLVLYINGIAVCVIELKRGSVNVTNG
                     IAQLLSNQKPEFNAWFFSTVQYVFAGNDSEGLRYGTIETEAKYFLTWKEDESENDLYK
                     LDKYLLKMCGKERLLDLIHNFVLFDGGKKKVPRVHQYFAIKAAQKNVRARTGGIIWHT
                     QGSGKSIVMILLSRWILANNPDARIAVITDRDELDKQMDRNFKNAGETIHKARSGRDL
                     LTQLAKPSPRLICSLIHKFGNKDAEDLDQLLKDLHAKPPKTSGEIFVFVDECHRTQSG
                     HLHKLMKAILPNAVFIGFTGTPLLKKDAATTLEVFGKYIHTYKFNEAVQDQVVLDLVY
                     EARDVDQRLTSVEKVDYWFEESSRRLKNKWQLDELKHTWGTMQKLLSSRSRMDKIIGD
                     IEFDFKTRPRLAGDRGNAILVASSIYEACKYYSLLQQTTFKNRCAVITSYNPQTKDIS
                     EEDMGANTETDKEFIYHTYTGILEDVVARPGKSKTETYEDQARNAFVDEPARMKLLVV
                     VDKLLTGFDAPSCSFLYIDKSMQDHGLFQAICRTNRLDGEDKQFGYIVDYKDLFKRVK
                     HALSVYTSELDHTAPGAEPEIMLKDRLATAREKLEDARQKYSILCEPVKPPRDYLDYQ
                     RYFCGNTEIPSDLEAREGYRMELYKSVAVLVRCYANLDTELEDAGYTKNQAEQIKQEV
                     KEAVALREKIRMAADEFLDLKPFEADMRRLIDNYIQADDPRPISPFGNLGLLDIIVKL
                     GIAEAISGHLGDFKGNKEAIAETIENNVRKKIIKEHLNDPNFYEKMSELLSEIIANRK
                     AKALDYEEYLKKIAKLIQTMQAGKTAEVPKTLDTKGKIALYNNLKQDEALALAIDAKV
                     RDVRPDDWRGVVPREQIIKKGLFEILGNEEEVNRIFQIIEQHTEY"
     gene            11299..12015
                     /locus_tag="PLG78_02225"
     CDS             11299..12015
                     /locus_tag="PLG78_02225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNE21950.1"
                     /translation="MVSTITLGDIVVQVTRKDIKNVHLSVYPPHGSVRIAAPRRMNLD
                     TIRLFAIAKLAWIKKQQRSLRAQNRETPREFLERESHYVWGKRYLLKIVEESGSPCIK
                     TKGKSLILRIKPGSSESRRDSALSDWYREQVREESALLLAKWEKRIGVKVKRVYVQHM
                     KTKWGSCNRQTGSIRLNTELAKKPPECLEYILVHELIHFLERNHGERFVALMNQHIPG
                     WRVYRNRLNRLPLRHEQWSY"
     gene            complement(12051..13016)
                     /locus_tag="PLG78_02230"
     CDS             complement(12051..13016)
                     /locus_tag="PLG78_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21951.1"
                     /translation="MMSQVGVQAACAVAILLIALSPARAEEILLKNGSTLSGRVTGQD
                     SMTVFVNTAQGMRSIPKAELVRIQYVSFTPEQKAQALDARRAKDAAAALEWERVRQLR
                     ETAERKRREDELEAKIRAEKEADARAASERAAALRELVKKGQMEKPADEPISYWDFAW
                     RSLVLPGWGHFYLDRPVFGAAYVAGTAGFVAAVYETHRRALSAVRENHREAQSNFILS
                     ATPVLPGFEMRMAYGLYSNAKAITVYQHKLDQYNGALAALATFYCVQVVHIIYNGIAW
                     ENGLLIVENRKDPGTIDAQFSAGPDYTEPGKKPGVAVVGALTMRF"
     gene            complement(13020..>13713)
                     /locus_tag="PLG78_02235"
     CDS             complement(13020..>13713)
                     /locus_tag="PLG78_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNE21952.1"
                     /translation="SANSGNGYAGIKTWRVPTRMEFETLVDHDASAAPWSFAAAFPAT
                     VSSMYWTSTAYQPSATKVWTLNFFTGFFGGNLKTNAYNVRCISGSPANPTYNYTDNGD
                     GTIKDNGSGLIWQKCSMGQNNDTTCSGAATTATLTPAMTYCNSLGLAGKSWRLPNIQE
                     LKTLIDTTTSASVKINGTAFPSTPAISNYHSSTTWNPAAGTSNWQIYLGDGLVDSTGK
                     TNAYNVRCVSGP"
BASE COUNT         3541 a         3357 c         3563 g         3252 t
ORIGIN      
        1 tggcgcgaaa gatccaggcg tcccttctgc cacgatttgt tcctgacgac gacaaatgcc
       61 gcatatcggt attattttcg cctgctgctt ccgtgggcgg agatctgtat gagtttcatc
      121 atcttcccaa ccactctgtt tctttttttg tgggagatgt gtccggccac ggcgttccag
      181 cagccatgct ttcttctctt gtgaaagcag cagtcagcat tcagtttacg gaaggaagac
      241 tttctccgcc gatttttctt tccagcatca atgaatttct ctgtcgtcag cttcgcggaa
      301 attttgtcac cgcgttttac ggccacattg atttaaaagc aatgaagctt gcgtatgcga
      361 ctgcaggaca tcctcacgcg tacctatgga atccggatcg cactaccgtg acgcgcattc
      421 gcacgcgagg aaagatgctc ggactgtttg ctgatctggt gtttgaagag gagattgttc
      481 cgctgcagcc tggagatcgc attctcatcc caacggacgg aattctggag tgcgaaaacc
      541 aggccggtag acttttcgag gaagagggaa tcgaaagagt gatttccgcg catgcggcgg
      601 cgccggatct gattgttcgc cttgaaaccg cgcttcgggc ctggactggt cttcgccccg
      661 gagacgatca aacggctctt cttgtggatt tgaaacagac tttgcaagaa tgatttgacc
      721 ataaggccac attcaagact ctggcccttc acggagttgt agctcagttg gttagagcgc
      781 cggtctgtca caccggaggc cgcgggttca agtcccgtca gctccgcaca tccgctttca
      841 ttccttccca cgcgtgcatc tcgtccggtc acaaacgcgg acgtccctgt ccgagtttgt
      901 gacgcttcgc cctccgtggc tacgcggcgg acggacgcac gcgtgggaag tgcaaacgga
      961 ttcgagcgcg gagctccttt ttcggaatag aacgcgcggc acgtccatgc gccgagcgct
     1021 gcgccggcaa ccgcgtgttg cttgcagagc aggacccggg tcagatcgtg cgacggagtg
     1081 agatttggtg cattttggaa aagtgacgct tcgccctccg tggcttgttt ttttctgtac
     1141 ctgaaagccg ggcctttttg aacctgggct tcgtggccca tcaaacattg tattccagcc
     1201 atgcttctgc tcctgtaatc caacagacga tcattgaccg ttatgcggaa ttgttccttc
     1261 ataacccggc ggactatatc aaacacaacg gaaacaactg gttcacagtg aagggcaatg
     1321 caacaccggc ggtgctggcc gcgcatctac gacatgagat tgtgattggc ctctctccac
     1381 tcaaagatgg aaccatccgc tggcttgccc tggacttcga tgcacacaac ggagagagct
     1441 attcagaaat cagtgccgat cttttggcga tcgtagccgc actgcgtcga gccgggattc
     1501 agtgtcatcc agaggcgtcg ggtcgcggct ttcatgtttg gatattcttc gccgtcccgg
     1561 ttcgtcccaa ttttattccg gcgttgaagc agttcgtcgg aaatcgctgt gaagtgtatg
     1621 ccggcaagac gcggattcgc attccgatgg gtgtctatca gaaagacaga tcgcgcttct
     1681 gcggcttcct cgatcattca ctcactgaga taccggacca gtttgagtat ctaaagaaca
     1741 tcaaaccaac gaaacctgat cagattgtcg cattgaatca tttgccaccg caaggaccgg
     1801 ctgccgtaaa aacaaaaatc acggttcatt catggtatcg caacaagccc gcgactgcga
     1861 ctccgaagaa agatggcagg caagtcgacc gcgaaaattt actgtttcac tttgagattc
     1921 aacccttcat caataacaga ttttcgagcg tccccaacct caagcacagg ctcgttctct
     1981 atctgggttt tgctctccaa ctaacggcaa aggagacggt gaaccttcgt gtgcgtgatc
     2041 tttcggaaaa tggaagcgag ctgaaagttg cggccccgcg gtgcaaagca aagacctatc
     2101 ttattactga ttgggctgcg tcgattttcc gcggtctctc tgaaggcaag gcgccaaatg
     2161 ttcttgtttt tcaagaaggg cggaagaatt ttagagagcg gtctgttcag aagattcttc
     2221 agagattttt cgaaagcacg ttgcgggggt gattcatcca ctgcttaacc cgaacttttc
     2281 tgcaatccga gcgagtctct tgtctgagct cacgaacctt tccaaagtgc ttcctgattc
     2341 cagcgcgcat gcgagttgaa gacaatcgag agttttcatc tggtgttgtt ttaagatgga
     2401 aagagctctt tgttcgacgg ggtctgaaaa tgctatcacg cgcattcgct ggctctctag
     2461 gtcgaaccag tcatagagct gttcggcatc tgtgacaccg atcgcatgat cggtttgtaa
     2521 gcgatttagt gctgaataga attccactcg cgtatgaaac gcgactatca cttcgctcgc
     2581 ggattcataa agttgcgctg cgagttccca cccattctgg cgcaaggcca cgagtgccag
     2641 tgcagatgtg tcaaagaaac ggatcacgcg ttgtagcgat cgtcctcgat gattttgacg
     2701 atatcgacgg gcttgagcaa attcactttt ggcggcagcg tatatctcgc tctgtcagat
     2761 acgttcatat ccagcgacgt agtgcttgca gcaaggaact ggccaaactg caatgcatgc
     2821 tggctccagt ttcgcccatc ttccaaaagt tgttcctgca cccttgccag ttcactttgc
     2881 atcattgagt ctcttttcaa gaattccgat tgcttccttt agttcagtga cctcatttct
     2941 gaggcgtcct ttctccgatt cgctctctgt taactttgtg agtaaatcct tgttggttgc
     3001 ggccattcga tctgcgtcgg ctttcagtac tgcgtaagct ttttgaacct gatgcagcat
     3061 tgcaattaca cgctcgcgat cttcggcgat ggcgcggagg cgttccccgg tccaatgcag
     3121 ggtcttttcc aggttattct tctctgtgta ttggagcaag ttcccgagca ggcttccgac
     3181 cgctaccaga cctgaccagt ccacctcttt gtcttcttcg tacgccatat gcccaggtac
     3241 aaaatgaaag catgaagatg gtttggccat cgttattttg cactgcggaa gcacgcgccg
     3301 ggttccgtgc gccttcttcc ctaaaaacct tgcacgctgg atgccctcac tcacgttcag
     3361 atggaaatgc ccatcaaaaa atcagaactg tattcatcca tctggcagag ctgcgatgaa
     3421 ctccgcggcg gcatggatgc aagccagtac aaagactatg ttctggtatt gctcttcatc
     3481 aaatacatca gcgataaata cagcggcggg gcattcgaac caatcaagat cccgaagggc
     3541 gccagcttca aggacatggt cgctctcaag ggcaaaagcg acattggaga caagatcaat
     3601 acgcagatca tcgggcctct gggaaaggca aacaatctgt cagacatgcc agacttcaac
     3661 tcagagaagc ttggaagcgg aaaggagaaa gtagaaaggc taggaaatct catttccata
     3721 tttcagaatc ctgctcttga tttctccggg aaccgcgcag agggagatga cttgctgggg
     3781 gatgcgtatg agtacctcat gcggcacttc gcaacggaga gcggaaagag caaaggacag
     3841 ttttacacgc ccgcagaagt gagtcgtgtg attgcccagg tcattggaat aggagaagcg
     3901 aaaacaactg catccactac tgtctatgat ccaacttgcg gatccgggtc gttgttgctt
     3961 aaagtggcag acgaagcccg cacaaaagta acgttatacg gtcaggaaaa agacgtggcc
     4021 acgtcgagtc tcgcgcggat gaatatgatc ctgcatgata atccggaagc gttgattgaa
     4081 aacggaaaca ccctggcaga tccaaaattc aaagaaaaag aagcactcaa agcctttgac
     4141 tatgtagttg caaatccacc gttctctgat aagcgctgga gcacgggcgt ggatacggag
     4201 aaggatccgt ttggtagatt tgaaacattc ggagtgcccc ctggaaagca gggcgattac
     4261 gcctacctcc ttcacattgt gcgttctctc aagaagaacg ggaagggtgc gtgcattctg
     4321 cctcatggag tattgtttcg cggaaacgcg gaagcggaga tccgcaagaa tctggtgaag
     4381 cacggctatg tcaaaggcat cattgggctg cctgcgaact tgttttacgg cactggaatt
     4441 cctgcatgca ttattgttct ggataaagaa gaagctcccg ctcgcaaagg gatcttcatg
     4501 atcgatgcga gtgcaggcta tctgaaagat ggaaacaaga atcgtcttcg cgcgcgagac
     4561 attcacaaaa tcgtagatgt cttcaagaac ctgcaggaag ttccaaaata cgcacgcatg
     4621 gtttctctcg cggagattga aaagaatgag ttcaatctga atttgcccag atatattgat
     4681 tcgcaggaag cggaggattt gcaggacata gaagggcatc tgcgcggcgg aattcctgcg
     4741 cgcgatgtgg atgccctttc ctcttactgg aagatttgtc ccggactccg gaaaactctc
     4801 tttaaagaga atcgaccggg ctatgtggac ctatctgtag aaaagtctgc catcaagtct
     4861 gcgatctttg atcatcctga gttcagcgcg ttcattcaaa aaatgaattc gcattttcag
     4921 acctggaaag agaaaaatac agcgcatctg aagaaactta aatccggctt tcatcctaaa
     4981 gaactgattg cctctctttc tgaggagctg ctttcgcatt acgagggtca gcctctgatc
     5041 gatccatatt ctgtgtatca gcatctcatg gactactggt cagagaccat gcaggacgat
     5101 ggttacatga tctctgcaga cggctggacg gcacagacgc agcgagtact cgtcaaagac
     5161 aaaagcggaa aagaaaaaga caaaggctgg gtctgtgact tgattccaaa agaactcgtg
     5221 gttcatcgat tcttcgcaaa agagcgcgaa accatctcca caatggaaag tgaagcggaa
     5281 ggcctggccg cgcaaattca ggaactggaa gaggagcaga ccggagaaga cggcgctttt
     5341 tctgaattgg agaaagtgaa ccgcgcatcc gtcgcggctc gattgaaaga gatcgcggga
     5401 gaggcggact ctaaagaaga aatccaggcg cttgaaacct ggacaaagct caacactcag
     5461 gaagcggagc tccgccggaa gattaaggaa ctagaaacag aactggacga tcttgcgtac
     5521 aagaaatatc ccaaactcac agaatctgaa gtcaagacac tggtagtgga ggacaaatgg
     5581 atggcggctc tggatgcaca agtccacggg gagatggata gaatcagtca gactctcaca
     5641 cagagagtga aagaactggc ggaacgttat acgaccactc tacctgaaat cacaaagacc
     5701 gtggcggacc tggagaaaaa ggtaagcgcg catctagcga agatgggttt tgcatggaac
     5761 tgaagccaag atacaagcac acggaagtcg gtgtgattcc ggaagagtgg gagctagtca
     5821 agctgcgctc cctttgccca aaatccgcgt acgggcccag gtttagttcc acgctgtata
     5881 gcgcgcacgg caacgtcggg acgttgcgca caactgacat ggatgattct gggaaaattt
     5941 cctacgagac aatccctttt gcgcacctgg atgaaaatct gttcaaagcg cacttcttag
     6001 aagacggcgc attcgttgtg actcggagtg gcacctgcgg catcgcggcc atttttcaca
     6061 agattgagcg acccgttgtt gccggcgctt ttttgatccg attccagttc aatgacacgg
     6121 tgcacagatc atttcttaaa gacatgctca attcgacgcg cggcagacgg ctcactgata
     6181 gcctgatcgc cggcggtgtt cagaaaaatc tgtcaggcac gggatttttg aatcttaaga
     6241 tccccctccc gccctttcca gaacaaaagg caatcgcaga agcgttaggc gacgttgacg
     6301 cgctcattga atctctggaa caactcatcg caaagaagaa gcagatcaag cagggcgcca
     6361 tgcaggaatt gctgacgggg aagaggcgat tgccggggtt cagtggagag tgggaagaga
     6421 agaagttggg ggatgtatgt gagattacta ccggtcgcaa ggatgttaat caaggaaatc
     6481 caaatggcat tttccccttt ttcacatgtt cacgagtgca cacgttcagc gattcctatt
     6541 cattcgacac tgaggctatc ttgatcgcgg gcaacggcga tgtcgggaat ttgaactact
     6601 ttgccgggaa gtttgaggcc taccaacgaa cttacgttct tcgcgaattc agtgtaccag
     6661 tctcatactt gtggcaccac ctcagtgcct tccttgcccc tgcgcttggc cttgataagg
     6721 ttggctcctc aattccatat ataaaaagag aaaatctcat tggtttcacg tatttgtctc
     6781 cacgtctagc cgccgaatca tccgccatcg ccaccgttct ctcagaaatg gattcggaaa
     6841 tctcagcagt ggaacaaaag ctttccaaaa cgcgtgccgt caaacagggc atgatgcagg
     6901 aattgctgac tggcaggatt agactggtat gacaagttca atcctgaaac tcacggctga
     6961 ctgtggagga aaagacgcct gcggcgcctt ttccaatgtt cttacgcgcg cgcgaaattt
     7021 gacgttatgc gatcactcgg ggtgcagggg tggacgttac gcgagcttgc ccctgctcgc
     7081 tccggggctc cggggggagt cgaaaccccc cggcgatggg gttccagggg ccggtcgctc
     7141 cggtccctgg ttcatgatgc aggaattgct cactggcagg attcgcctgc tatgacaagt
     7201 tcaatcctga aattcacgac tgactgtgga ggaaaagacg cctgaggcgc ctttcccaat
     7261 attcttatgc gcgcgaaaat ctaacgttat gcgatcacct ggagcaaaga gacaactatg
     7321 aagaattact atcgagtgat ggccgggaaa ggaagttctc ttgcagaaga atgtttccag
     7381 cagggatttg tgggagtaga ttacggagtg gccgtggatc tcaccggtaa attcccagat
     7441 gactggcggc agttcaatcg cgagttcatt cctgtctact tgcagagtca tccggagaaa
     7501 accaggatcg ctgccggctc agcctgcgga gctctctgga ctctggggag gggcatgcaa
     7561 aagggcgatg ttgtctttgt tccggacggg gaaggcagct atcatgtggg cgaagtcacg
     7621 agcgactatc agtatcaggc gggaaaaaat ctcccgcata gaaggcaggt tcgatggtat
     7681 ccagagatgg tgcggcgtga tgctacaagc ccgcaaatgc agggatcaac tcgctccaca
     7741 ggaaccattg tagacatcac tggctacgca aatgaactcg agaaacttct ttccggaaag
     7801 ggacccgctg ttttggtttc tacagatgag actgtagaga atccttccgc cttcgctctc
     7861 gaaaagcatc tggaggattt tctcgtggcc aactggaagc agacagagct tggaaagaat
     7921 tatgatattt acgaagaaga tggggaactt acaggacaac agtaccagac agacactggg
     7981 cccattgaca ttctggccat cagcaaggac aaaacggatt tgcttgtcgt tgaattgaaa
     8041 aagggcagag ccagtgacgt ggtcgtaggg cagactctgc gttatatggg ctatgttcag
     8101 gatgagctgc gcgaaccgaa ccaaaaagtc tctggcatta tcatagctct ggaagatgac
     8161 cccaaactga aacgggctct ggcaatgacg cctgcgattc agttctttcg atatgaagtg
     8221 agcttcaagc tgcgcaaggc gtgaaatgac ctctctcaac accaaagagc gcgccacgca
     8281 gaatcgcatc attcagcttt ttaagaattc tctgggctac gcgtacctgg gcaactggga
     8341 agagcgcgaa ggcaatagca acattgaaga agagcattta gctcgctggc ttgcaaagag
     8401 aggctttcaa aaagaacaca tcagctctgc tatttacaaa ctcaaaacgg aagcgcacaa
     8461 tcccaatcga ccgctggttc acaacaacat ggctgtttac agcatgctcg tctacggcgt
     8521 ggatgtgaaa ctggatgcta caaagaatac ggagcaggta ccgctcattg actgggagaa
     8581 tccggatcaa aacgattttg caattgcaga ggaagtcagc ctgcatggag ccgcagagag
     8641 gcgaccggac ctggtgctct acatcaatgg aattgcagtc tgtgtgatcg aactcaagcg
     8701 cggaagtgtg aatgttacca atggaatcgc acagcttcta tccaatcaaa agccggagtt
     8761 caatgcgtgg ttttttagca cagttcagta tgtcttcgcg ggcaacgatt cagaagggct
     8821 cagatacggc acaattgaaa cagaggcaaa gtacttcctc acctggaaag aagacgaatc
     8881 agaaaacgat ctttacaaac tggacaagta tctgttgaag atgtgcggga aggagaggtt
     8941 gctcgatctc atccacaact tcgtgctctt tgatggagga aagaaaaagg tgccgcgtgt
     9001 gcatcagtac ttcgccatca aggctgcaca gaaaaacgtt cgcgcacgta cgggcggaat
     9061 catctggcac acgcagggaa gtggcaaaag cattgtgatg attcttcttt ctcgctggat
     9121 tcttgcaaac aatccagatg cgcgaatcgc agtcatcacg gacagagatg agctggacaa
     9181 acaaatggac agaaacttca agaatgcagg agagaccatt cacaaagcgc gaagcggccg
     9241 cgatctcttg actcaactgg caaaaccaag tccgcgtttg atttgttctt tgattcataa
     9301 attcggcaac aaggatgcag aggatttgga tcagcttcta aaagatctcc atgcaaaacc
     9361 tccaaagaca tccggcgaaa tctttgtgtt cgtagacgaa tgccacagaa cgcaaagcgg
     9421 acacttgcat aaactcatga aggccattct tcccaatgca gtattcattg gattcacagg
     9481 tacacctctg ctcaagaaag acgctgccac tactctcgag gtctttggaa aatacattca
     9541 cacctacaaa ttcaacgaag cagtccagga tcaggtggtt ctcgatcttg tttatgaagc
     9601 gcgcgacgta gaccagaggc tgacatccgt ggaaaaggtg gactactggt ttgaagaatc
     9661 ttccaggcgt ttgaagaaca aatggcagct cgatgaactg aagcatacct ggggcaccat
     9721 gcagaaactg ctcagttcca gatcgcgcat ggacaagatc atcggtgaca ttgaatttga
     9781 tttcaaaacc cgtccacgtc ttgccggcga tcgtggcaat gcgattcttg tagcttccag
     9841 catctatgaa gcatgcaagt actactctct cctgcaacag actactttca agaatcgatg
     9901 tgccgtgatt acatcctaca atccgcagac caaagatatt tcagaagagg acatgggcgc
     9961 aaacacagag acggacaaag aattcattta tcacacatac actggaattc tggaagatgt
    10021 tgttgccaga cccggcaagt cgaaaacaga aacgtacgaa gatcaggctc gcaatgcatt
    10081 cgtagatgaa cctgcgcgca tgaagctcct ggtcgtagtg gataaactgc tgaccggttt
    10141 tgacgcgccg tcctgttcgt ttttgtacat agataaatcc atgcaggatc atggcctgtt
    10201 ccaggcgatt tgtcgcacga atcgtttgga tggtgaagat aagcagtttg ggtatatagt
    10261 ggattataag gatcttttta aaagggtcaa gcacgcactt tccgtgtata cttccgaatt
    10321 ggatcatacc gcgccaggtg cagagcctga aatcatgctc aaagacagac tcgccactgc
    10381 acgcgagaag ttggaagacg cccggcagaa atattcaatt ctttgcgaac ctgtgaaacc
    10441 gcccagggac tacctggact accagcgcta tttttgcggg aacacagaaa taccttccga
    10501 cctggaagca cgcgaaggtt atcgtatgga attgtacaaa agcgttgcgg ttcttgttcg
    10561 ctgttatgcg aatctggata cggagcttga ggacgcaggt tacactaaga atcaggcgga
    10621 gcaaatcaag caggaagtga aggaagcagt ggcgcttcgc gaaaagattc gaatggcagc
    10681 agatgagttt ttggatctga agcctttcga agcagacatg cgccgcctta tagacaacta
    10741 cattcaggcg gatgatccgc gacctatttc acctttcggg aatctcggtt tgctagacat
    10801 catcgtcaag ctcgggattg cagaggcaat ctccgggcat ctgggcgatt tcaaagggaa
    10861 taaggaagct atcgcagaaa cgattgaaaa caatgtgcga aagaagatca tcaaggaaca
    10921 tctcaatgat ccaaatttct atgagaagat gtctgagctt ctaagcgaaa tcattgcaaa
    10981 tcgaaaagca aaagccctgg actacgaaga gtatctcaag aaaatcgcga agctgattca
    11041 gacaatgcag gcgggcaaga cggctgaagt accaaaaacc ttggatacaa aagggaagat
    11101 cgcactttac aacaacctca agcaggatga agcgctcgca cttgcaattg acgcgaaggt
    11161 tcgtgacgta cgtcctgatg actggcgcgg agtggttcct cgcgaacaga tcatcaagaa
    11221 aggtcttttt gaaattctgg gaaatgagga agaagtgaac aggatctttc aaatcataga
    11281 acaacataca gagtactgat ggtatctaca attacactcg gcgacattgt ggttcaagtg
    11341 acgcgcaagg acatcaagaa tgttcatttg agcgtttatc ctccacacgg gagtgttcgc
    11401 atagcggcgc cccgcagaat gaatctggat acaatccgtc tttttgcaat cgcgaaactg
    11461 gcctggatca agaagcaaca gcgtagtttg cgtgcgcaaa atcgggaaac tccgcgtgaa
    11521 ttcctggaac gcgaaagcca ctatgtgtgg ggaaagcgtt atctcttaaa aatcgtagaa
    11581 gaatcaggaa gtccatgtat caaaacaaaa ggcaaaagtt taattctgcg aatcaaaccc
    11641 ggctcctccg aatcgcgcag agacagtgcc ctctcggact ggtaccgaga gcaggtgcgc
    11701 gaagagtctg cgctgctgct tgcaaaatgg gaaaagagaa ttggggttaa agtgaagagg
    11761 gtctatgtgc agcacatgaa gacaaagtgg ggtagctgca atcgccaaac cgggtccatt
    11821 cggctgaaca cagagcttgc caaaaaaccg ccggaatgtc tggaatacat ccttgtgcac
    11881 gagctaatcc attttctaga acggaatcat ggggaacgat tcgtcgcttt gatgaatcag
    11941 cacattcccg gctggagagt gtatcgcaat cggctcaatc gactgccgct gagacatgag
    12001 cagtggagct attgaaccac aaccgttcgc catcgcacac cccacccctt tcaaaaccgc
    12061 atcgtcaaag cacctacaac agctactccc ggttttttcc ctggttccgt gtaatcaggt
    12121 cctgcgctga actgcgcgtc gatggttcct ggatcttttc tattctctac tatcagcaga
    12181 ccattctccc aggcgatgcc gttgtagatg atgtgaacca cttgcacgca gtagaacgtc
    12241 gccagggctg cgagcgctcc gttgtactga tcgagtttgt gctggtatac tgtgattgcc
    12301 tttgcgtttg agtacaaccc gtacgccatc ctcatttcga agccaggaag gacgggcgtc
    12361 gcagacagaa tgaaattgct ctgcgcctct ctatgatttt cgcgaactgc actgagcgct
    12421 cttctatgcg tctcgtatac agctgcgacg aatcccgctg tgcccgccac gtatgctgct
    12481 ccgaatactg gtctgtccag gtagaagtgt ccccatccag gcagaacgag cgatcgccag
    12541 gcaaaatccc agtaagagat gggttcatct gcaggcttct ccatctgtcc tttttttacg
    12601 agttcgcgaa gtgccgcagc gcgctctgag gcggctcgcg cgtctgcttc tttctctgca
    12661 cggatttttg cttcgagttc atcttcgcgt cgtttgcgct cagcggtttc tctgagttgt
    12721 cgcacgcgct cccattccag ggctgctgct gcgtccttcg cgcgtcgcgc atcgagtgcc
    12781 tgtgcctttt gttccggcgt aaaagaaacg tactgaatcc taacgagctc tgctttgggg
    12841 atgcttcgca ttccctgcgc cgtgtttacg aacactgtca ttgaatcctg tccggtcacg
    12901 cgtccgctca gagtggaacc attcttcaaa agaatttcct ctgcgcgggc aggcgaaagc
    12961 gcgatgagca ggatggcgac tgcgcatgct gcctgaactc caacttgcga catcatatgc
    13021 tacggtcccg agacgcaccg aacgttatac gcatttgtct tgccagtgga atccacaagc
    13081 ccgtcaccca gatagatctg ccaattgcta gtacccgcgg ccgggttcca ggtcgtggag
    13141 ctgtgataat tactaatcgc gggagtagat ggaaatgccg tgccatttat ctttacgctc
    13201 gcggatgtag tggtatcgat gagagttttc aattcctgaa tgttcggcag tcgccaggat
    13261 tttccagcaa gaccaaggct gttgcaatag gtcatggcag gtgtcagtgt ggccgtggtt
    13321 gccgctcccg aacacgttgt gtcgttgttc tgccccatac tgcatttctg ccagatcagt
    13381 cctgagccat tgtctttgat tgtgccatct ccattgtctg tgtaattgta agtcggattg
    13441 gcaggagatc cggatataca acgaacgtta tacgcattcg tcttcaggtt gccgccaaaa
    13501 aatccggtga agaaattcaa ggtccatacc ttggttgcgc tcggctggta ggcagtggaa
    13561 gtccaataca tgctcgacac agtcgccggg aatgcagcgg cgaatgacca gggcgccgcc
    13621 gacgcgtcat gatcgactaa cgtttcgaat tccattcttg taggaactct ccatgttttg
    13681 atgccggcat atccgtttcc ggagttggca gag
//
LOCUS       DAOQJL010000028        13529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2213_length_13529_cov_4.435283, whole genome shotgun sequence.
ACCESSION   DAOQJL010000028 DAOQJL010000000
VERSION     DAOQJL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_13529_cov_4.435283"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLG78_02240"
     CDS             complement(<1..609)
                     /locus_tag="PLG78_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21953.1"
                     /translation="MENFDKIGQTEMMKVPHFHLDEFQTRVLNNLHLEFFLKKCRFII
                     TPDYRYLLTGEADEKETGILDYKIPEKREALEKLKLLLIYDLSLYSALLETNSYYIAS
                     NGHLLICRFNTVKDHPEAFELKLYTISRDDLPENYKDKIYLGRDYISIKNLRKEHFGL
                     RYMRNSLIEQLMHLRARMMELLPEETREDLEEEFLSEVEELIG"
     gene            complement(646..1665)
                     /gene="queA"
                     /locus_tag="PLG78_02245"
     CDS             complement(646..1665)
                     /gene="queA"
                     /locus_tag="PLG78_02245"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNE21954.1"
                     /translation="MLEEYSFDLPEDLIARHPPRERDAARLLLVDPVRGEFREESFRN
                     LPNLLQPRDCLVLNRSKVSFRRLHVQRRDRTFEPLFCSMKEGAWRVLVYGAAKLKTGD
                     ELRHESGRLFKVIGRDEDFVLLEPDVDWSRESWEDFFERFGRVPIPPYFGREDNEEDR
                     IRYRTIYGQSGESLAAPTAGLHFTQEILDRLALKQIDVAALDLHVGFGTFAPLKPWNF
                     EQHRLHLESFEIDDSCATAIENASRVIAVGTTTARALESVTRRHGKIQKANEDTDLFI
                     FPPDSLKSVQGLITNFHLPSSSLFMLVCAFGGRQLMHRAYQFAIEQRFRFFSYGDAMM
                     ILPGI"
     gene            1770..3260
                     /locus_tag="PLG78_02250"
     CDS             1770..3260
                     /locus_tag="PLG78_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE21955.1"
                     /translation="MGTWKRTLQRLGDLFSTSRAVRIGCLCLLLLAIAVTSLRIIKDP
                     SSSGLIASSRSDFDDYYTASGMVGRGEDPYRVEQLQGIMELPRSFRPEDLLNPAKLIE
                     LMSRFKGLGTYLYLPFTAFVLLPLSGLDYKVAAGIFQFLSIFAFVAFIAFLHRSLRRD
                     RPLITEREFTLPLLAAFSLLGGFLSENAANGNIAFFLLLLTGPGIYYSFSDKWWAQVA
                     GGMLIGTAAVMKVTPVFLVLVLAASIRVYALVGVGLGIGLGIFLPALGLGWDRNLALL
                     QGWYNLILETYNKYVFLRPWANNQTISGMIGKLFLPGSDLKQSEYGLPLIGGLPDKQM
                     YASLGLLVKGINAFLLGITGIASVVSAIRLFKNGFQSLVRSVFGSFDARPAFFEDVSM
                     VRLLWLVTLVSLVTAGVSWYHAYCVLLLPWFLRFVQVFSGEKLLLGEKVAIAFTAFFS
                     LGPLMMGSHLREGLALYSIFTFGVCFQALYLAWLVIASNRRPADGI"
     gene            3250..4758
                     /locus_tag="PLG78_02255"
     CDS             3250..4758
                     /locus_tag="PLG78_02255"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE21956.1"
                     /translation="MVFNSGVYIYFLIAVFTIYWAVSRLPRGAHRAQNTVLLLSSYFF
                     YGWWDWLYLVLIFASTTLDYFVGIGLDSLTSERKRKLLITFSISCNLTILGIFKYYDF
                     FITSFISMFRQFDPGAFPDGGAHLLLNAILPVGISFYTFQSMAYTVDVYRRQVRAERN
                     FLDFASFVCFFPQLVAGPIERAGDLLVQMKNNRQFTWENMRNGAWMILLGFYMKTYVA
                     DNISPLVNLVYLDHKFAYHQNPLAASGHGGLQVILASVGFAFQIYSDFWGYSAIAMGS
                     ALFLGFKLTQNFNAPQMAENPADLWRRWHITLNRWVTDYIYIPLGGSRLGVVRKYVNL
                     FVAMLLMGLWHGASWTFFVWGGFMGAWLIAHQIFRDYLPKLSEGAPAWKKGTVKALKM
                     AATFGVFGLSATCFRAYDIHQTGELWKSILDISSYSLSPVHGVMGAGDFLWALVKKLI
                     VLLVIDFMAYRTGSLYWIFDRPVWVRITLYSVLFFFVLILGEFGKDVIYFAF"
     gene            4736..5986
                     /locus_tag="PLG78_02260"
     CDS             4736..5986
                     /locus_tag="PLG78_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21957.1"
                     /translation="MSSILRFKYPALLGAVVVIAILEIAARIIPLEYTSGAGIFLTEK
                     RRALIEASSPEYDYIILGDSRSLSLNGHPRTDKEPYTVYNLSIPAMGPRYFQYYLEKY
                     LHNRKHKPAAVVFAGDPGLFQRSWYTPNHDSKMIYADSVEEGLGHYLWSRFHKRIQYL
                     KAGKYPDQRDQFGAMVWEVFSHRYLHLFSVSDMMEQYTGAERIFMLRESIPNAFYLYR
                     YRDGIRQYTFDLRANSFRKHDLPDFCNACSGLVRAECHPDYSRVEDNQKLDALIEKNY
                     GGVNLGDRLDPAQRFNYYQMRDTAVEHQVDWFSREEPEMKPVEDLIRTAAKHDIKIVF
                     SDVPAVEPMRTVRYHKIYFERLAQIVSKYPGARIVRFPDPYYPVKLFIEQVHYECEGA
                     ARLNQEFYSSVMPEVVKFAPRSAP"
     gene            5983..7071
                     /locus_tag="PLG78_02265"
     CDS             5983..7071
                     /locus_tag="PLG78_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 domain-containing protein"
                     /protein_id="HNE21958.1"
                     /translation="MKSNLRVVYFPLILFLVYFGLDKTCLLPQMKKLSQPDATYLYFD
                     YKTDLIDQVEKIHKANQKPENFVSGVRKKTLIILGSSRLLYFDTARFHRNYPDWELFN
                     FSAPVTSPAYYAYIFERILERGVKPDYVVVEVDPLQYNDGSDHFVRSNLAYSFDLPFI
                     LKHHDLFKNSEISFFLARFLFAGYKYPPDLQAIASRARDPNDRWLLALNELDKYQREN
                     RGAGRSIIPRENWYERDFARLELSARHTISWLYGNFELSERQFGFLEMLLRDARSAQV
                     PVLLLRPQVSSTMQRQLDEDSRLAAPLAEWEVRLKKEQKDFSVPYLDLRHDRDVSCNT
                     FVDGAHMALDCYHMLTVAVMREYRANGL"
     gene            complement(7058..7534)
                     /locus_tag="PLG78_02270"
     CDS             complement(7058..7534)
                     /locus_tag="PLG78_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE21959.1"
                     /translation="MTARQSVIAFYVALAAIVAGGGMLLFYNFHTLSRNAEPVIAVTR
                     SGLRFYREESAARSEAEKTGKPLFIEFGALWCEPCLQFNEKMDKDPALQTALSGAVLV
                     RIMDTDPTFESYSSRKGYEALADGLPFFVIESRGEIKLASHEWKNTAAIARAIKGR"
     gene            complement(7531..7995)
                     /locus_tag="PLG78_02275"
     CDS             complement(7531..7995)
                     /locus_tag="PLG78_02275"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNE21960.1"
                     /translation="MNRIAFFALICSLFALFSCRDEKQAVPEKETHQTTVTAGAKGDR
                     LTIAVSVPAGHHAYLDSGKEGVLIPISIDWKEAIAKKFIQATPRSVAIPDGVQDEQMG
                     ARVLRGKGDFVYEARFDKLEGQTIKVRSQICDDVKGICYRPTTQDIPVVSVK"
     gene            complement(8038..9033)
                     /locus_tag="PLG78_02280"
     CDS             complement(8038..9033)
                     /locus_tag="PLG78_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNE21961.1"
                     /translation="MPKEITHIILAEQMREKLPRDVGELLHSHAQEYFFGSTAPDLFY
                     YDVPTPFDASPSSEIISETLHGRMGNKNNEHIFWMLDRMKKLDQQDRYFAFIAGYLSH
                     IAADTIFHPMVYSRTGNYFDLDPAERKRSRARHRVLETSLDLYLLSKSGRTLRGLGLL
                     RLLRVPEDFSRSLMNFFSLSLSEIYHPDLAVSRGALRSVLKSRLIIAMTLSPIAYKFF
                     SWINRILSRKHNDLINLFYPPKPAAIDFETSAQIPHPITGNPYPSRIEELRSMATSRG
                     IAYIAAASAYWKGTISDTELRAAIPPLSLNSGMQCTTVESMIHYEILPGIEQFDD"
     gene            complement(9033..9962)
                     /locus_tag="PLG78_02285"
     CDS             complement(9033..9962)
                     /locus_tag="PLG78_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21962.1"
                     /translation="MMKDFLIAVKRTKWERDLRTYGSEEKIRQIYQIQNNAYDRVFAS
                     HQRQKESFELVKQTLKNSDFFFREDLQSASFENYRCIASLGGDNHFVYVSRFAKSPIA
                     GINSDPKSSRGALLAGNASEFVQFYSQFDSTPEEDKASFLQKRITERWSRIECTIIFP
                     NGDAQNIGACTSEFSIRNRFAEYMSRYLIRKRLAHSEEGAPPFKEEKCSGLLCATGAG
                     STGWFKSCLPVEERNHAVFSRSAPFFEYLAREALQPMPPARVQSDEALEIISEMDGEI
                     VADSDHELSFSFPYGARAILKLAGERLSVIHEF"
     gene            complement(9959..10714)
                     /locus_tag="PLG78_02290"
     CDS             complement(9959..10714)
                     /locus_tag="PLG78_02290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNE21963.1"
                     /translation="MQETTPLTSRTKLPSAAEKSDYVRTNFDAIARNYDRFNDWISFG
                     MHRLWKKRMVQETGLTGKKARVLDLCCGSGDISLAFARILADGSEITSLDFSHEMLNV
                     LSHRLLTFPSRGVQIRPLEGDAMKLNYSSEFDAASMSFGLRNVTDRALCLRGILRALK
                     PGAPFVLLDVGKPPSIFMPFFRVFFEGIVPKIGHWIHGGKHSMYDYLPASARAYPDQK
                     AMKKELEDAGFVNVRIINFSLGAAGLHVARKPE"
     gene            10729..12627
                     /locus_tag="PLG78_02295"
     CDS             10729..12627
                     /locus_tag="PLG78_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21964.1"
                     /translation="MAVTKQQADSATGQPFEVLDLQQSTEFVQALEARGDFKFKGIFK
                     VFQTILDWTGRFSSTNILPSSDQIAKDVEIVQDRLNIYLLELQGRGSSKEKFVEAIPY
                     IDLSTEGLVPMVVFARPTKQDAGSARRYTDAYRDKSLASIKKYLAYRRPLQYDDFEKL
                     IRTYVESGKLAELQAGSEISRLFFQDPDPNVRKKYHTHYVIPIYRKLVEEKLFVALPE
                     LKPRILLYNVSDELWNRFFILADILLSSLGESGKFSDRPDPRELGSYLNGYDPGRVEG
                     MRPGYKQAFQELVLLCGLLEKRTEEERERKKKEELQKLSDEIGKLPRVVELRKFERYP
                     EEVRNQLLRSGSVLQAEVPVGSRMGHFVLHKQRVKDAVKNARDAFDQTGDPVEVRILS
                     AMSVEDFLTGDEVRAFHDLEQRTYMEHLPWLVRVWRALFGNAKLKPEEIKKIKQKVDR
                     EQQEASLRIRKQEADKSKRELAAKKLKGEDGKPEAAPAKPREEETVTTEEDLVHKNVV
                     MDEKIQETVRGMVTELDHAWDKNMLPNRNFLIEKYPIFNEETIINFLKKYGRTEILSF
                     RIMHEKPEYLWPILISRRYVKAKGKAMLAKAKHDADEQRKAMMPNQEKFDIATALEDF
                     LNRILPKM"
     gene            12653..>13529
                     /gene="pyk"
                     /locus_tag="PLG78_02300"
     CDS             12653..>13529
                     /gene="pyk"
                     /locus_tag="PLG78_02300"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE21965.1"
                     /translation="MAQKKAKKTGRKHVEKVIAEDVVSLPLQIVPVRRDFRLTKIICT
                     IGPASEATTMLEKLARAGMNVARINMSHGTREKHREIIRRIKNLNRKLNHPIAILMDL
                     QGPEIRTGELKKNINLAVGEIFTFNILPEENPEERSVFVNYKGMVDQLKAGDRITVDN
                     GLINLQVLEKSLTGLRCVVLEGGTLGSRKHINLPGVRVNLPSLAEKDKRDIEMALQED
                     LDFIALSFVRSADAVTEARAMVESSGGHTRIISKIENEEGVANFDSILAESDGIMVAR
                     GDLGVEVEIAELPVIQ"
BASE COUNT         3235 a         3168 c         3483 g         3643 t
ORIGIN      
        1 acctatgagt tcttctactt ctgatagaaa ctcttcttcc aggtcttccc gagtttcttc
       61 gggaagtagc tccatcatgc gggcgcgcaa atgcatgagc tgttcaatca aagagttccg
      121 catgtagcgg aggccaaagt gttctttgcg caggttctta atggaaatgt agtcgcggcc
      181 caggtaaatc ttatctttgt agttttccgg aagatcgtcg cggctgattg tatagagttt
      241 gagctcaaac gcttccggat gatccttcac ggtattgaac ctgcaaataa gcagatgtcc
      301 gttggaagct atgtagtacg aattagtttc gagcagggcg gaatagaggg agagatcgta
      361 gatcaggagt agctttagtt tctcgagggc ttcgcgcttt tccgggattt tgtaatccag
      421 gatgcctgtt tccttttcat ctgcttctcc agtcagcaaa tagcggtaat caggcgtgat
      481 gatgaatctg catttcttca ggaagaattc caggtgcagg ttattgagca cgcgcgtctg
      541 gaattcatct agatgaaaat gaggaacttt catcatctcg gtttgaccga tcttgtcgaa
      601 gttttccatg cctttcaatg tcccttgcgc aacgacgccg gtcaatcaaa ttccgggtag
      661 tatcatcatc gcatctccgt aactgaaaaa gcgaaatcgt tgttctatcg caaattgata
      721 cgcgcgatgc atgagctgtc tgccgccaaa ggcgcaaacc agcataaaga gagaggaaga
      781 agggagatga aaatttgtga taagtccctg gacagatttt agagaatcgg gcggaaaaat
      841 aaaaagatcc gtgtcttcgt ttgccttctg gattttgccg tgtcgcctgg tgactgattc
      901 aagagcccga gcggttgttg tgccgaccgc aatgactcgc gaggcattct cgattgcagt
      961 ggcgcatgag tcatctattt cgaatgattc caggtgaagc ctgtgttgtt caaagttcca
     1021 gggtttgaga ggagcaaaag tgccaaatcc cacatgcaag tcgagggccg ctacatcgat
     1081 ctgttttaga gcgagtctat ccagaatttc ctgtgtgaaa tgcagacctg ctgtgggggc
     1141 ggccaggctt tcgcctgatt gtccgtaaat ggtcctgtat cgaattctat cttcttcgtt
     1201 gtcttcgcgc ccaaagtagg gtgggatagg cacacgcccg aatcgctcaa aaaaatcttc
     1261 ccaggattct ctggaccaat caacatccgg ctcgagcaaa acaaaatctt cgtctctgcc
     1321 gatcactttg aacaggcgac cgctttcgtg tcgcagctca tctcctgttt ttaacttcgc
     1381 ggctccatat acgaggactc gccaggcgcc ttccttcatg gaacagaaca aaggctcgaa
     1441 ggtccggtcc cgtctttgta cgtggagcct gcgaaatgaa actttgctgc ggttgagaac
     1501 cagacaatct cgaggttgaa gaaggttcgg caggttgcga aatgattcct cgcgaaattc
     1561 gccccgcact ggatcgacga gcaggagcct cgctgcgtct ctctccctgg gaggatgcct
     1621 ggcgatcagg tcttcgggca gatcaaagga gtattcctca agcatagcct gaaattcagc
     1681 gtctgtcacc gggctagagt tcgaggaaga ccttctgtgc ccaggagaat tttcatttca
     1741 aaagctcgta tgatcaggaa aattggacga tgggcacctg gaagcgcacc ctgcagaggc
     1801 tgggcgatct tttctccact tctcgcgcgg tcaggatcgg ctgcctgtgc cttttgctac
     1861 tggcaattgc cgtgacttct cttagaatca tcaaagatcc ttcttcgtct ggtctgatcg
     1921 cttccagtag aagcgatttc gatgattact acaccgcgtc tggcatggtc ggaagagggg
     1981 aagatccata tcgcgtggag caattgcagg ggatcatgga gctacccagg agctttcgcc
     2041 cggaggatct tctcaatcct gcgaagctca tcgagctcat gagtcgcttt aaggggctgg
     2101 gcacctatct gtatttgccc ttcacagcgt ttgttcttct tcctttgtct ggcctggatt
     2161 acaaagtagc tgctggcatt tttcaatttc tgtctatttt tgcgttcgtt gcctttattg
     2221 catttttaca tcgttctctg cggcgagaca ggcctctcat cacagaaaga gagtttactc
     2281 tcccacttct tgccgccttc tcattgttag gtggtttcct cagcgaaaac gcggccaatg
     2341 gcaacatagc gtttttcctt ttgcttctta cggggcccgg gatttactat tccttttcgg
     2401 acaaatggtg ggcccaggtg gcaggtggca tgctcattgg cacggcggca gtgatgaagg
     2461 tgactccggt gtttctggtt ctcgtgcttg ccgcttcgat tcgagtgtat gcactcgtgg
     2521 gagtgggcct tgggattggc ctcgggatct ttcttcctgc tcttggtctg gggtgggatc
     2581 gcaatctggc tttgttgcag ggttggtaca acctgattct tgaaacctat aataagtatg
     2641 tgttcctcag accctgggca aacaaccaga cgatttctgg aatgatcggt aagttatttt
     2701 tgccaggcag tgatctcaaa caaagcgagt atggtcttcc actgatcgga gggctgccgg
     2761 acaagcaaat gtatgcttct ctggggctgc tcgtcaaagg catcaacgct ttccttctgg
     2821 gaattacggg cattgccagc gttgtcagtg cgattcgcct ctttaagaat ggctttcaaa
     2881 gtctggttcg atccgtgttc ggcagtttcg atgcgcgccc tgcttttttt gaagatgttt
     2941 ctatggtgag actcttgtgg cttgtgaccc tcgtttctct cgtgactgca ggagtgtcct
     3001 ggtatcatgc gtactgtgtt cttctacttc cctggtttct tagatttgtc caggtgtttt
     3061 ccggcgagaa gcttctgctc ggggaaaagg tagcgattgc gtttactgca ttcttttcgc
     3121 ttggaccact gatgatgggt tcgcatttgc gcgaaggtct ggcgctctat tccattttta
     3181 cattcggcgt ttgttttcag gcgctctatc tggcatggct cgtaattgcg tcgaacagga
     3241 gaccggccga tggtatttaa ttccggcgtc tatatttact ttctcatcgc agtattcacc
     3301 atctactggg ccgtttcgcg actgccgagg ggcgcgcatc gcgcacagaa tactgttctt
     3361 cttttgtctt cgtatttttt ttacggatgg tgggactggc tctatcttgt tttgattttt
     3421 gcgtccacaa ctctggatta ttttgttggg attggcctgg atagccttac gtctgagaga
     3481 aagcgaaaac tgctgatcac cttctccatc tcgtgcaatc ttacaatact tgggattttt
     3541 aaatattacg atttctttat caccagtttc atttcgatgt tcaggcagtt cgatccaggc
     3601 gcattcccgg atggaggagc ccacctcttg ttgaatgcga ttttgcccgt agggattagc
     3661 ttctatactt tccagagcat ggcttatact gtggatgtgt acagaagaca ggttcgcgcg
     3721 gagcgcaact tccttgattt cgcttctttt gtttgttttt tcccacaact tgtagcgggt
     3781 cccattgaga gagccggcga tctgctggtt cagatgaaga acaatcgtca gtttacctgg
     3841 gaaaacatga gaaacggtgc atggatgatt ttgctcggct tctacatgaa gacctacgtt
     3901 gcagataata tttctccgct cgtcaacctg gtgtatctgg atcacaaatt cgcatatcac
     3961 cagaatccac tcgctgcaag cgggcacggc gggttgcagg taattcttgc aagtgtaggg
     4021 tttgcgtttc agatctattc tgacttctgg ggatactccg cgatcgccat gggctccgct
     4081 ctatttctgg gattcaagct cactcagaac ttcaatgcgc cgcaaatggc ggagaatcca
     4141 gcggatctct ggaggcgctg gcacatcact ctcaatcgct gggtcaccga ctacatttac
     4201 attcctcttg gaggaagcag gctcggagtt gttcgcaaat atgtgaatct ctttgtcgcc
     4261 atgttgctga tgggcctgtg gcacggagca agctggacat tttttgtctg gggtggtttc
     4321 atgggagcgt ggctgatcgc acaccagatc ttccgcgatt atcttccaaa gctttctgaa
     4381 ggggcgcctg cctggaagaa ggggacagtg aaggctttga aaatggcggc cacgttcggt
     4441 gtgttcgggc tttctgccac ttgctttcgc gcctatgaca ttcatcaaac aggtgagctc
     4501 tggaaatcga ttctggatat ttccagctac tcgctttcgc ctgttcatgg agtgatgggt
     4561 gcgggcgatt tcctctgggc actggtcaag aaactgatcg tcttgcttgt gattgacttc
     4621 atggcctatc gcactggatc tctttactgg atttttgatc gtccggtgtg ggttcgcatc
     4681 actctgtatt ctgtgctttt ctttttcgtt ctgatcctcg gggagtttgg caaagatgtc
     4741 atctattttg cgttttaagt atccggcact cctgggcgcc gtggtggtca ttgcgattct
     4801 cgagatcgca gctcgaatca ttcctcttga gtacacttcg ggcgctggaa tctttctgac
     4861 tgaaaagaga cgcgccctca tagaggcttc ttctcctgag tatgactata taattctggg
     4921 cgattcgcgc agtctcagtc tgaacggcca tccgcgaaca gacaaggaac cctacacagt
     4981 ttataatctg agcataccgg ccatgggtcc gaggtacttt cagtactact tagaaaaata
     5041 cttacacaat cgtaagcata aaccagcagc cgtcgttttt gccggagatc ctggtctttt
     5101 tcagagaagc tggtatacgc ccaatcatga ttcgaagatg atttacgcgg attctgtaga
     5161 ggagggtctg gggcattacc tctggtccag gtttcacaag agaattcaat acctgaaagc
     5221 gggcaaatat cccgaccaga gggatcagtt tggtgcgatg gtatgggaag tcttctcaca
     5281 cagatatttg cacttattta gtgtttcaga catgatggaa cagtacactg gtgcagagcg
     5341 aatcttcatg cttcgtgaaa gcatccccaa cgctttttac ctctatagat atagagatgg
     5401 aatcagacag tacacgtttg acctcagggc caattcgttt cgcaaacatg acctgcctga
     5461 tttctgcaat gcatgttctg gcctggttcg cgctgaatgt catccggatt attccagggt
     5521 agaggataat cagaaactgg acgcactgat tgagaaaaac tatggtggag tcaacctggg
     5581 ggacagactc gatccggcac aaagattcaa ttactatcaa atgcgagaca ctgcagtcga
     5641 gcatcaggtg gactggtttt cgcgcgaaga gcctgaaatg aagccagtgg aagatttgat
     5701 ccgcacagct gcaaagcatg atatcaagat tgttttctca gatgtgccgg ccgttgaacc
     5761 tatgcgaacc gttcgatatc acaaaattta ttttgaaaga ctcgctcaga ttgtaagcaa
     5821 gtacccgggt gcacgaattg tacgcttccc ggatccgtac tatccggtga aactgttcat
     5881 agagcaggtt cactatgaat gcgaaggtgc agccaggctc aaccaggagt tttactctag
     5941 cgtgatgcct gaagtggtaa agtttgcgcc gcggagcgct ccatgaaatc caaccttcgc
     6001 gttgtttatt tccccttaat cctgtttctc gtttactttg gtctggacaa gacctgcctt
     6061 cttcctcaaa tgaagaagct atctcagcca gacgcaacct atctatactt cgattataag
     6121 actgatttga tcgatcaggt agagaagatt cacaaagcaa atcaaaagcc tgaaaacttc
     6181 gtttccggag tcaggaagaa aaccctcatc atcctcggca gttccaggct attgtacttt
     6241 gatactgcgc gttttcatcg caactatcct gactgggaat tgttcaattt cagcgcgcct
     6301 gtaacctctc cggcgtatta tgcgtacata ttcgaacgca ttctagagag aggagtgaag
     6361 cctgattatg tggtcgtgga agtagatccg cttcaataca atgatggatc cgatcatttt
     6421 gtaagatcca acctggcgta cagttttgat ttgcctttca ttttgaagca tcacgatctt
     6481 tttaagaatt cggaaatttc attcttcctg gctcgattct tgttcgcggg ttacaaatat
     6541 cctccggatc ttcaggcaat cgcttcgcgc gctcgcgacc cgaacgatcg ctggcttctt
     6601 gccttgaatg aattggacaa gtaccagaga gagaatcgcg gagcaggcag atcgattatc
     6661 ccgcgcgaaa actggtacga gagagacttc gcgcggcttg agctgtctgc gcgtcacacc
     6721 atttcctggt tgtatggaaa ttttgaattg tctgaaaggc aattcgggtt tctggagatg
     6781 cttctgcgag atgcccgctc agcccaggtt cctgttctgt tgcttcgtcc ccaggtgtct
     6841 tccacgatgc aaagacagct agacgaagac tctcgtctgg cagcacctct ggctgagtgg
     6901 gaagtgcggc tgaagaaaga acagaaggat ttttccgtgc cgtatctgga tttgaggcat
     6961 gaccgcgatg tgtcgtgtaa caccttcgta gacggagcgc acatggctct ggattgctat
     7021 cacatgctca cagtggctgt gatgcgagag tatcgcgcta acggcctttg atcgcccggg
     7081 caatggctgc tgtatttttc cattcgtgac tggccagttt gatttctccg cgtgattcaa
     7141 tgacaaagaa tggaagtcca tctgccagtg cttcatatcc ttttcttgag gagtaagact
     7201 caaaggtagg atccgtatcc atgattctca caagcaccgc tccagagagc gcggtttgca
     7261 gggccgggtc tttgtccatc ttctcattaa actggagaca gggttcacac cagagcgcgc
     7321 cgaattcaat gaagagtggc ttgcctgtct tctctgcttc gcttcgcgcg gcggattctt
     7381 ctctatagaa tcgcagtcca ctcctggtca cggcgatcac tggctcagca ttccgggata
     7441 gcgtgtgaaa gttgtaaaac agtaacattc cgccaccggc cacgatggcc gcaagcgcca
     7501 catagaacgc aatgacggat tggcgagcag tcatttcacg gaaacgactg gaatatcctg
     7561 agtggtgggt ctatagcaga ttccttttac gtcatcgcag atttgagagc gcactttgat
     7621 ggtctggcct tcgagtttat caaatctcgc ctcgtataca aagtcgcctt tgccgcgaag
     7681 gacgcgtgcg cccatttgtt cgtcctggac tccatctggg atggctacag atcgcggagt
     7741 tgcctggatg aattttttag caatcgcttc tttccaatcg atggaaatag gaatcaatac
     7801 tccttctttg ccagaatcga gatacgcatg atgaccggcc ggcacagaca ctgcgatggt
     7861 aagcctgtct ccttttgctc cggctgtgac tgtagtctgg tgagtttctt tttcaggtac
     7921 tgcctgtttt tcgtctctac aggaaaagag cgcgaagagt gaacaaatga gagcgaaaaa
     7981 tgcgatccgg ttcattcttt cagaaaaacg atttgatcct ttgaggcaac aaaaaactca
     8041 atcgtcgaac tgttctattc ctggcaaaat ttcgtagtga atcatgctct ccacggttgt
     8101 gcactgcatt ccgctgttga gagacagcgg tggaatcgcg gcacgaagct ctgtgtctga
     8161 aatcgtgccc ttccagtacg cgctggccgc tgcaatgtat gcgatgcctc gcgatgtagc
     8221 catggacctg agttcttcaa ttctggaagg ataaggattc ccggtgatcg gatggggaat
     8281 ctgtgcggac gtttcaaagt caatggccgc aggcttcgga ggatagaaga ggttgattag
     8341 atcattgtgt ttgcgtgaaa gaatgcggtt gatccaggaa aagaatttgt acgcgatagg
     8401 cgaaagagtc atggcaatga tgaggcgcga tttgagaaca gagcgcaaag cacctctaga
     8461 aacagccaga tccgggtgat agatttccga aagagaaagc gaaaagaaat tcatgagact
     8521 gcgggaaaaa tcttcaggga ctctcagtag cctgagcaga ccaaggccac gaagtgttct
     8581 tcctgatttg gaaagcaaat agagatcaag cgacgtttcg agcaccctgt gtcgcgctct
     8641 ggagcgcttc ctttccgctg gatccaggtc aaagtagttt cctgttctgc tgtataccat
     8701 gggatgaaag attgtgtctg ccgcaatgtg agaaagatac cctgcaatga acgcgaagta
     8761 gcgatcctgc tggtccagtt tcttcattcg atccagcatc cagaagatgt gctcgttgtt
     8821 tttgtttccc attcttccgt gcagagtttc tgaaatgatt tctgacgacg ggcttgcgtc
     8881 aaagggagtg ggaacgtcat agtagaagag atcgggtgcg gtgctgccga aaaaatattc
     8941 ctgtgcatga gaatgtagga gctcgccgac gtcgcgcggt agcttctctc gcatttgctc
     9001 tgccagaatg atgtgcgtga tttctttagg catcagaatt catggatgac cgaaagtctt
     9061 tcccccgcga gcttcaggat ggcgcgagct ccatagggga aagagaagga aagttcatga
     9121 tctgagtcgg caactatttc gccgtccatt tctgaaatga tttctagagc ctcatccgat
     9181 tggacgcgcg cgggaggcat gggttgaagt gcttcgcgcg ccaggtattc aaagaacggt
     9241 gcgcttcttg agaacacagc atgattcctt tcttctaccg gtagacagct tttgaaccaa
     9301 ccggtggatc cagcccctgt ggcgcagagt agcccggagc atttctcttc tttgaacgga
     9361 ggtgctcctt cttctgaatg ggccagtctc tttcggatca ggtatctgct catgtactct
     9421 gcaaagcgat ttctgatgct aaattcgctt gtgcaagctc caatgttctg tgcgtctcca
     9481 tttggaaaga tgatggtgca ttcaatgcga ctccatcgtt ccgttatgcg cttttgcaga
     9541 aaggaggctt tgtcttcctc gggcgtggag tcgaactgtg agtaaaactg aacaaattca
     9601 gatgcattcc ctgccagaag tgcgccgcgt gagcttttag gatctgaatt gattcctgcg
     9661 attggacttt tagcgaatct gcttacgtac acaaagtgat tatctccgcc aagtgaggca
     9721 atgcatctgt agttttcgaa agaagcagat tgtaaatctt cgcgaaaaaa aaagtctgaa
     9781 ttcttcagag tttgtttgac cagctcgaaa gattcttttt gtctttgatg agatgcaaat
     9841 actcgatcgt aagcgttgtt ctggatctga tagatctgtc taattttctc ctcagaaccg
     9901 tacgtgcgca aatcgcgctc ccattttgta cgcttgactg cgatgagaaa gtctttcatc
     9961 attctggttt gcgggcaacg tgaaggccag cagcgcccag tgagaaattg atgattcgca
    10021 cattcacaaa tccggcgtct tcgagttctt tcttcattgc cttttgatcc ggataggctc
    10081 gcgcagatgc tggaaggtag tcatacatag aatgctttcc gccgtgaatc cagtggccga
    10141 tcttcggaac aattccctca aagaagacac gaaagaaagg catgaaaatg gaaggtggtt
    10201 ttcctacatc gaggagaacg aaaggcgcac caggtttcag tgcccgtaga attcctctta
    10261 gacacagggc gcggtcggta acatttcgaa gtccaaagct catgcttgcg gcatcgaatt
    10321 cgctcgaata gttgagcttc atcgcatcgc cttccagcgg ccgaatttgc acaccgcgcg
    10381 atggaaaagt taaaagcctg tgcgaaagga cgtttagcat ttcgtggctg aagtccagac
    10441 tcgtgatttc tgaaccgtct gccaggattc gtgcgaacgc caggctgatg tccccgcttc
    10501 cgcagcatag atcgagtaca cgcgcctttt ttccggtaag gcctgtttcc tggaccattc
    10561 gctttttcca cagtctgtgc atgccaaagc taatccaatc gttgaaccgg tcgtaattgc
    10621 gggcaatggc gtcaaaattt gttcggacat agtccgactt ttcagccgcg gaaggtagtt
    10681 ttgttctgct tgtcagggga gtggtttctt gcattctcgg ctcatccaat ggcggtgacc
    10741 aagcagcagg cagacagcgc caccggccag ccttttgagg ttctggatct ccagcaatct
    10801 acggagttcg tccaggcact agaggctcgc ggtgacttca agttcaaagg aatcttcaaa
    10861 gtattccaga ccattctgga ctggactggc cgcttttcgt ccacgaacat cctgccttca
    10921 tccgatcaaa tcgccaaaga tgttgagatc gtccaggaca gactcaacat ctatcttctt
    10981 gaactccagg gccgcgggtc gtcaaaggaa aaatttgttg aggcgattcc gtacattgac
    11041 ctgtctacag aaggcctcgt gcccatggtc gttttcgcgc gtccaaccaa gcaggatgcg
    11101 ggatctgctc ggcgctatac ggatgcgtat cgcgacaaga gtcttgcctc tatcaagaag
    11161 tacctggctt acagacgtcc tcttcaatat gacgattttg aaaaattgat tcgaacctat
    11221 gtagaatccg gcaagctggc ggaactgcag gcaggatcag agatctctcg tctcttcttt
    11281 caggatccag atccaaacgt gcgaaagaag tatcacactc attacgtaat tcccatttac
    11341 agaaagcttg tggaagaaaa gctattcgtc gctcttcccg aactcaagcc gcgcattctg
    11401 ctctacaacg taagcgatga actctggaat cgctttttca ttctcgcgga cattctactt
    11461 tcgtctctgg gtgaatccgg taaattttcg gacagaccgg acccaagaga gctcggcagt
    11521 tatctcaacg gttacgatcc tgggcgagta gagggaatgc gtccgggcta caagcaggca
    11581 tttcaggaac tggttcttct ctgcggtctt cttgaaaaac gtacggaaga agaaagagag
    11641 cgcaagaaga aggaagaact tcagaaacta tctgatgaaa ttggaaagct gccgcgcgtg
    11701 gtggagcttc gaaaattcga aagatatccg gaagaagttc gcaatcagct tcttcgttca
    11761 ggctcagtgc tgcaggctga agtccccgtt ggttctcgca tgggacactt tgtacttcac
    11821 aagcaacgcg tgaaagacgc agtcaagaat gcgcgcgacg catttgatca gaccggagat
    11881 ccggtggaag tgagaattct ttctgctatg tctgtggaag attttcttac cggagatgag
    11941 gtgcgtgcat tccatgactt agagcagcgc acctatatgg agcatttgcc ctggctcgtg
    12001 agagtatggc gtgctctttt tggaaacgcg aagctcaagc cggaagaaat caaaaagatc
    12061 aaacagaaag tggatcgtga gcagcaagaa gcaagtcttc gcattcgtaa acaggaagcg
    12121 gataagagca agcgtgagct ggcggccaaa aagctgaaag gagaagacgg taagccagaa
    12181 gcagctccgg ctaagccgcg cgaagaggaa actgtcacta cagaagaaga tctggtgcac
    12241 aagaacgtgg tcatggatga aaagatccag gagacagtgc gcggaatggt tacagagctc
    12301 gatcatgcct gggacaagaa catgctccca aatcgtaatt tcttgattga aaaatatcct
    12361 atcttcaacg aagaaacaat catcaacttc ctgaagaagt acggaaggac tgaaatcctt
    12421 tcgttccgca tcatgcacga gaagcccgaa tacctctggc ccattctcat ttcgcgtaga
    12481 tatgtaaagg caaaagggaa ggcaatgctt gcgaaggcca agcatgatgc agacgaacag
    12541 agaaaagcga tgatgccaaa tcaggagaaa tttgacatcg cgacagcgct cgaagatttt
    12601 ctaaaccgca ttttgccgaa aatgtaatca acgttcccca cggactaaat caatggcaca
    12661 gaaaaaagca aaaaagacag ggcgaaagca tgtcgaaaaa gtgatcgctg aggatgttgt
    12721 ttctctgcca ttgcaaatcg ttccggttag acgcgatttt cgcctgacca aaattatctg
    12781 cacgattggc cccgcctccg aagcaacaac gatgcttgag aagctggctc gcgcaggaat
    12841 gaacgtggcg cgcatcaata tgtctcacgg cacacgcgag aagcatagag aaatcatccg
    12901 cagaattaag aacctgaatc gaaaattgaa tcaccccatc gccatactga tggatttgca
    12961 gggaccggag attcgcacgg gtgaacttaa gaagaatatc aacctggccg tgggtgagat
    13021 ctttaccttt aacattttgc cagaagagaa tccggaagaa agaagtgttt ttgtaaacta
    13081 taaaggtatg gtggaccagc tgaaagcggg agatcgcatc acggtcgaca atggcctgat
    13141 caacctgcag gttctcgaga aatctcttac cggccttcgc tgtgttgtgc tcgaaggcgg
    13201 cacacttggt tctcgcaagc atatcaatct tcccggggtg agggtgaacc tgccatctct
    13261 cgcggagaaa gacaagcgcg acattgaaat ggctctgcag gaagatctcg attttattgc
    13321 tctctcattt gtgcgaagcg cggatgcggt tacagaagcg cgtgcgatgg ttgaatcgag
    13381 cggcggtcac accagaatca tttccaagat tgaaaacgaa gagggcgttg cgaattttga
    13441 tagcatactt gccgagtccg acggaatcat ggtggcccgt ggcgatctgg gagttgaagt
    13501 ggaaatcgcg gagcttccag tcatacagc
//
LOCUS       DAOQJL010000029        13405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2245_length_13405_cov_4.396404, whole genome shotgun sequence.
ACCESSION   DAOQJL010000029 DAOQJL010000000
VERSION     DAOQJL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_13405_cov_4.396404"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLG78_02305"
     CDS             <1..206
                     /locus_tag="PLG78_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21966.1"
                     /translation="VLLKHAGSIADAHSRLAPLLSAEMIRDAAALVPDSWFIKHGREE
                     YIDFVQKRLSSGPFAEEAERVRS"
     gene            193..582
                     /locus_tag="PLG78_02310"
     CDS             193..582
                     /locus_tag="PLG78_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNE21967.1"
                     /translation="MSDREAYQYAMWRVVPLLERGEFINAGVVLFSRRHRFLKAQVHL
                     DEVRLKAICPDVDLENVRSQLTFREKIAAGDPEGGSVALQSQSERFGWLVAPASTVIQ
                     PSQVHTGLCDDPEAALSHLFKRLVMIA"
     gene            complement(579..1340)
                     /locus_tag="PLG78_02315"
     CDS             complement(579..1340)
                     /locus_tag="PLG78_02315"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNE21968.1"
                     /translation="MTAAAAYRKTIHKCARVIESTFYDLHRKGQKENLHEFRLAVKRL
                     RALLPSLPSPPSLVLLSPAFRAAGRIRNIDVQRSILEETLDGRRMPDNLKQFLKRRRK
                     KARRRLRTITRMIPADTLEDLVRAIQRHTNQEPDTGLEESIRKNIEGRITDVNESASQ
                     TMNAEQIHALRAKVKEAAILSMLLPPGSTPLVPFEKTADLLGKWHDCVVTLKTLDRFR
                     KKHLEAELKNLPDLVDRMKDRYFASAMSLMNAPPR"
     gene            1456..2700
                     /locus_tag="PLG78_02320"
     CDS             1456..2700
                     /locus_tag="PLG78_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNE21969.1"
                     /translation="MLRSRLVSGFLIVVGALLAAEIGLRVLQVDSLQYYYDMKRLHAF
                     HPDYLVGLAPNQDRTIRHYAGLWQGRFTTNSLGYRGSPEPDGSPVIACLGDSLVMGFG
                     VSDEETFCAKLAGINLHGKKHQAMNLGVDAFGSVGDYKRLKEASGQLNLDTVLLFVSP
                     NDFTIPPELAKRGVRPDDLIDEDRKNHPELTRFFRIQFEATRISYLLHALKLSREQLA
                     VTMVTRRESVRSQMVQAGLSQPEEGDRISSSTGRYILEQFYAFKKHATCEPEKTSNAQ
                     ASCPEPVPDQAGYACIPRPAASELEPLPAITMEAYRNMISLAREKNFRLVPVFLPVQV
                     EELYCDMHGKHHALGNYAVRAQAFFEKEGIRTIDLADKVRDICKLEKHSVLDQIIPGD
                     GHLTKSGNIWAARALREKLNAL"
     gene            2690..4117
                     /locus_tag="PLG78_02325"
     CDS             2690..4117
                     /locus_tag="PLG78_02325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE21970.1"
                     /translation="MLFNSVHFLIFAPVVMAVTFLLPQRLQRWWLLLASLYFYAVANV
                     VFLIPLLFCIGITYVLVRWMVSVESQVQKRIALLGVIFGNLGILYSLKYMDLSFWVWN
                     HTLGLRPCDPWYAHSLGMILPMGISFFTLMAIGYGVDVYRGFEGRRSFLDTALFLSVF
                     FHLVAGPILRGRDLIDQFAQKIGWSWENFELGMRQICLGIVKKTFLADNISPTVDAIF
                     AAPAQMHWVSLLLSVFLHSFQIYCDFSGYSDIAIGSARILGFHIPENFNRPFLSLTMT
                     EMWRRWHISFSSWVRDYVYFPLGGSRTSPPRAYFNLFMTMFVSGIWHGPTMNFVLWGV
                     MHAVWLVLEKLLFSVDRIKKAYDSIPSILKIAYTFTAFSLALFFFRARMLPGFSSEME
                     VGFFMLKRAFTLVPGELVLPPAGVAVGILILWILEAIVEKNEHVFAPLWERKGVCAAG
                     AVALLFISFCIYSVTVSAQFIYFQF"
     regulatory      4118..4246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4371..6386
                     /locus_tag="PLG78_02330"
     CDS             4371..6386
                     /locus_tag="PLG78_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE21971.1"
                     /translation="MFAQEIEVTGKKRTLGDQPAATSEIPVSDYAGRFETLEDILEKE
                     TSVRVKRYGGPGSQSSISVRGSNANQTNLYLDGIPLNNAVSGEANLADYNLDGVGSIQ
                     VFRSGQSSGFSGSAIGGSVNLVPERGESGTRLRLRGGTERTIDVAARAGGSRNPEEAE
                     KEETEKAKVDPSLLDAMRAQWSVSGHTGRSDQTYKYRNNNGTPVFNTFDDHDDRRKNA
                     WYRDDGFTGYASAGVGNTDLKILDDFTYRMHGVPGPGSRQTEKTHRELTRNTTGIGSD
                     TRGLGVDWIRLETRAYYTYYRESFFDPLQEFSTSSPNSSGKLDVYGIHLMPSLYFPVA
                     FQTIRFLFAEEREDYHQEIRNRLDQKTENVPARFRRHTSLHAEDEFSFFKKRLIITPS
                     VRYELYRDEFPDFRFRSYSEFGNPEKRRIDYTNASLFSSIVLARISQAEFRFRAGGAK
                     EERMPTFLELFGERGSVIGNSNLKPEKSESSEGGFSLVADRQPMSGEFSITLFRKNIR
                     DMILFVPNSQFSLRPENVDAARITGVEASAKMFIMKRLRLYATYTYQRAINMSDVSYL
                     KGKYLPLRPLHDLHSGLSIYAGKWEMGAEADFTGAVFRDRTNEYLSYQAGRWVYNAYL
                     TFAPLGRGERELIFGLEMKNVLDQRMEDVIGYPLPGRTLYGTINYKF"
     gene            6361..7530
                     /locus_tag="PLG78_02335"
     CDS             6361..7530
                     /locus_tag="PLG78_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21972.1"
                     /translation="MERSTTNSRFSFRIALPWLGVILVSFAVSGCKDIERPPLWTLFS
                     ASTGSARVGVVTTDFGSNGRFSVITTEGFPLPGLSPIYSDAVARYQNNQVTVINRLGR
                     DNIQVLEPAIGLLTVQEFSLGSGSNPHDYIRLSDAKGYAALYNRAYVQIVQPATGFLI
                     GTISLAAYADVDGIPEADSMLQEGSSVYVALERLDRNQTNLPPVGVSKLVEINSGSDA
                     IVAAYDFPSANPFGKMRRVVLGGVPHLVVPCPNRLGFLSAMDGGVVAFNLNTRTFRPG
                     FLYAETTAGGDILDVEIASDTVGYATVLDAVFNKSLQKFNPTTGQKLAVLSFSPSSAG
                     TISGLFLAPNGYLYAGDASFSQPGVRIYDTQRNDALVTPVPVSVGLRPFDFVYLP"
     gene            7581..8270
                     /locus_tag="PLG78_02340"
     CDS             7581..8270
                     /locus_tag="PLG78_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21973.1"
                     /translation="MMRVDRDSLRFSMSACFALAYLFSPGLFAEIRVDSSSQVFYKNA
                     TAMLGRMKDSPDPEIRKLYQEVSRASVTVHIRPITDDSRTWHVDGDRTRAHTEALDNL
                     GRGRARNRPTSAAIYLTTESVDPTRTSWKGGVLPHEMTHAMDLASGRYNKDYKIRERR
                     AIVFQNIWRARVNKTLREDYHGSFDTLDYQNAKRAGTLGKLTEHIFSSSDLPSSRDSN
                     DEGDDDDGDEE"
     gene            complement(8289..9284)
                     /locus_tag="PLG78_02345"
     CDS             complement(8289..9284)
                     /locus_tag="PLG78_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21974.1"
                     /translation="MGIREDFNHSRYIPGQKKGHYESFFQRANHPTRPLAFWIRYTVF
                     SPENDPASAIGELWAIYFDGEKNSHIAVKKEVPIRECEFDRNRFGAKVADAFLNQSEL
                     AGQVKQGKNSISWNLSFGSDAQPLFDFPESMYSTALPKAKVLVGMPLARFDGSLTVNG
                     QKVQVKSWLGSQNHNWGSKHTDHYAWGQVAGFDHSEDTFLELATAKLKFGPIWTPALT
                     PIVLRHRGQEFRLNQLIKTMGRASFGFFDWDFHASSAEIKIKGRITAKREDFVCLKYY
                     NPPGGFKYCLNSKIARCDLEILSSSSSHAEMLTSKSRAAFEILTDSNDHGMSPAC"
     gene            9399..11435
                     /gene="tgt"
                     /locus_tag="PLG78_02350"
     CDS             9399..11435
                     /gene="tgt"
                     /locus_tag="PLG78_02350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNE21975.1"
                     /translation="MNGRLDFQLEAEASDSHARAGRFRTLHGEILTPLFMPVGTNATV
                     KGLRFEDLAQSGSQILLANTYHLLLRPGPEVFQTIGGIHNLTHWRGSFLTDSGGFQIF
                     SLSHARSMSEEGAAFRSYVDGRMIVLTPELSIATQKAIGSDIMMVLDQCIPSTADFET
                     AKRAMELTHRWARRSLNARGESPQSMFGIVQGAMYSELRRQSAEVLTQMEFDGFAIGG
                     LAVGETKAQREEFTEFTASLLPRDRPRYLMGVGTPIDLLEAVHRGVDMFDCIIPTALS
                     QQGVAYTSRGKMRLNRTVYKFAEEKLDPACDCYTCRTYSLAYLHHLKKSSELLGWQLV
                     SIHNLHFYHRLMASMRTHILAGTFRTFYEKAREELVREDEERPKGPRPRVRSTKNYES
                     RGAFSVHRTREGSMSIKDSASGEIMHPGADPGIEADRLYVNQSRLADRLREEGKEESE
                     LVIWDVGLGAATNAMAAVRAWELVQSENENVRRLRIVSFENDLDALNLALLHTGNIKH
                     LRHSAPREISMNGVYRSENGRLIWELLHGDFLELLGSAVVPDLIYFDPFSYKTNPSLW
                     TLETFARVFQRCSEHDTELFSYSASTRVRSALLAAGFYVASGLSSGEKSETTIAMTEQ
                     ALLRRKGSTEDELHLLNERWLERWQRSDAPYPLLLTVDEEPGVRSLILNHKQFR"
     gene            complement(11489..11830)
                     /locus_tag="PLG78_02355"
     CDS             complement(11489..11830)
                     /locus_tag="PLG78_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNE21976.1"
                     /translation="MKLITAMVQPHKVEDVKQALYKAGVTKMTVLNAMGCGQQKGYDE
                     NYRGAVHTVNLLRKVVFEIAVNEDYVEPTIKAVIEGARTGKIGDGKIFVTELKEVIRI
                     RTGERGKEAIG"
     gene            complement(11848..13389)
                     /locus_tag="PLG78_02360"
     CDS             complement(11848..13389)
                     /locus_tag="PLG78_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE21977.1"
                     /translation="MKSDFKKWSYFLVLGAVLVSARLYAETPAAPVPAGPTVDQRLAD
                     LEAYINNGAVGAAGTAKWTSNVPGSGPGHNGWMMVCSALVLFMTLPGLALFYGGLTRQ
                     KNILSVMAQCLGIAGVVTILWWAAGYSLVFANDSPTDATGALTPIVTLFGSLKHAFLR
                     GVDSVPNTDYAYWVSHNVFSMYQLMFAIITPALIIGAIAERMKFSAIIIFVTLWMFAV
                     YFPLAHAVWGINGMMNGVWNSAAKIHAIDFAGGTVVHMSSGWSALILCIILGKRIGFG
                     TKAMDPHNLVLTMVGTGMLWVGWYGFNAGSAVAADGVSANAFMTTTLATAVASFVWPF
                     LEYIIKRKPTVLGFCSGAVAGLVVITPACGFVNASGSIIIGLLAGSIPFFAVYKLKGW
                     LGYDDALDTFGVHAVGGTMGALVTGFLAVPAKFADGKVNPGAVNANLASNIDAFINSG
                     TLWIEQLKAIGFTLVLSVVATAVIAYLIKFTIGLRPSEEVETLGLDISEHGEEGYRGF
                     QMWND"
BASE COUNT         3122 a         3313 c         3500 g         3470 t
ORIGIN      
        1 atgttctttt gaaacacgca ggctccattg cagacgcaca cagtcgcctc gcgcctcttt
       61 tgagcgcaga gatgattcgg gatgcagcgg cacttgtgcc ggattcgtgg ttcatcaaac
      121 acgggcggga agaatacata gacttcgtac agaaacgttt gagttccggg ccatttgcag
      181 aggaggctga gcgtgtccga tcgtgaagca taccagtacg ccatgtggcg cgtcgtccct
      241 ttgctcgaaa gaggcgaatt catcaatgca ggtgtagttt tgttttcacg caggcataga
      301 ttcctcaagg cgcaagttca tctagatgaa gtgcgtctca aggcaatctg tccggatgtg
      361 gatctggaga acgttcgttc gcagcttacg tttcgcgaaa agatcgcagc cggtgaccct
      421 gagggtggat ccgttgctct tcaatcacaa tccgagcggt ttggctggct tgtggctcct
      481 gcaagcacag tcatccaacc ttcgcaggtc cacaccggcc tttgcgacga tccggaggcg
      541 gcgctctcgc atttgttcaa aaggctggta atgatcgcct aacgtggcgg ggcgttcatc
      601 aggctcatcg cggatgcgaa gtacctgtct ttcattctgt ccaccagatc gggtagattc
      661 ttgagttcgg cttctagatg ttttttgcgg aatcgatcga gagttttgag agtgaccaca
      721 caatcatgcc actttccaag caagtctgct gttttttcga atggaacaag aggggtgctg
      781 ccgggcggta gaagcatact gagaatggcc gcttccttta ccttcgcccg taatgcgtga
      841 atttgttccg cattcattgt ttgcgatgca gactcattta catccgttat tcgcccttcg
      901 atgttcttgc gaatcgattc ttcgagcccg gtgtcaggtt cctggtttgt atgtctttga
      961 atcgcgcgga ctaaatcctc aagtgtatct gctggaatca tccgtgtgat ggtgcgaaga
     1021 cggcgcctcg cttttttccg tctgcgtttg agaaattgct tcagattgtc tggcatccgc
     1081 cggccatcga gtgtttcctc gaggatagaa cgctgcacat ctatgtttct gattctaccg
     1141 gcggcgcgaa aggcaggcga aagcaaaaca agagagggtg gagatggaag ggaagggagc
     1201 aatgcccgaa gtcgtttcac ggccagcctg aattcgtgca gattctcttt ctgtcctttc
     1261 ctgtgaagat cgtagaaggt agattcaatg acgcgcgcgc atttatgaat ggtcttgcga
     1321 taggctgcgg cggcggtcat tcttcaagtt gcccctgtcg ggcgttttct ggccatgaga
     1381 aaacgagtaa aacgtgatgg attgaggtcg catggttttg tttacatcct gcttcgattt
     1441 tggaattctg cctccatgct ccggtcaagg ctggtgtccg gttttctcat cgtcgtggga
     1501 gctctacttg ccgcggagat tggcctgcgc gtcctgcagg tggattctct tcaatattat
     1561 tacgacatga agcgtttgca cgctttccat ccagattatc tggttggact cgctcccaat
     1621 caggatcgca ccattcgtca ctatgcaggt ctctggcagg gtcgcttcac taccaattca
     1681 cttggttatc gcggtagccc ggagccggac gggagtccag tcatcgcctg cctcggagat
     1741 agcctggtga tgggatttgg cgtaagcgac gaagaaacct tttgcgcgaa gttagccggc
     1801 ataaaccttc acggaaaaaa gcaccaggca atgaacctgg gtgtggacgc gtttggctct
     1861 gtgggagact acaaacgact caaagaagca tccggccaat tgaatctgga tactgtgctc
     1921 ttgtttgttt ctccaaacga ttttacaatt ccgcctgagc ttgcgaagcg tggagtgaga
     1981 cctgacgatc tcatagacga agacagaaag aatcatccgg agttgacccg attttttcgc
     2041 attcaatttg aggcaacgcg catttcttat ttgcttcatg cactcaaatt atcgcgagag
     2101 caacttgccg taaccatggt tacgcgacga gaatctgttc gctctcaaat ggttcaggct
     2161 ggcctttcgc agccggagga aggggacagg attagttcga gcaccgggcg ctatatcctg
     2221 gaacaattct acgcctttaa gaaacacgca acctgtgagc cagaaaaaac ttcgaacgca
     2281 caagcatcct gcccggaacc tgtgccggat caggcgggtt acgcgtgcat tcccaggcct
     2341 gccgcgtcag aactggagcc tctccctgcc atcaccatgg aagcatatcg aaacatgatt
     2401 tccctcgcgc gagaaaagaa ttttcgcctg gttcctgtct ttctccccgt tcaggtcgaa
     2461 gagctctact gcgacatgca tggcaagcac catgcactcg gaaactatgc agtgcgtgcc
     2521 caggcattct ttgagaaaga aggcattcgc acgattgatc tggcggacaa ggttcgggat
     2581 atttgcaaac tggaaaaaca ttctgtactg gatcagatca tcccgggaga cgggcacctg
     2641 acaaaatcag ggaacatctg ggctgcccgg gcattgagag agaagctgaa tgctctttaa
     2701 ttcagttcat ttcctcatat tcgcgccagt agtcatggca gttacgtttt tgcttcctca
     2761 gagattgcag aggtggtggc tccttcttgc aagtctctat ttctatgccg ttgccaacgt
     2821 agtttttctc attcctcttc tattctgcat tggaatcacc tacgtactcg ttcgctggat
     2881 ggtttcggtg gaaagtcagg tgcaaaaaag aatcgcactc ctcggtgtga tttttggaaa
     2941 cctcgggatt ctatattctc tcaagtatat ggatctttct ttctgggttt ggaatcacac
     3001 actgggtctt cgtccctgcg atccatggta tgcgcattcc ctgggaatga tccttcccat
     3061 gggcatttca tttttcactc tcatggccat tggttatggc gtggacgtat atcgaggatt
     3121 tgaaggaaga cgcagctttc tggatacggc actcttctta tctgtgttct ttcatctggt
     3181 ggccggacca attttacgcg gacgagattt gatcgatcaa ttcgcgcaga agataggatg
     3241 gagctgggaa aattttgagc tcggaatgcg gcagatctgt ctcgggatcg tgaagaagac
     3301 attccttgca gataatatca gtccaacggt agatgcgatt tttgcagcac ccgcgcagat
     3361 gcactgggtt tcacttttgc tttctgtatt tctgcattct tttcagattt actgcgattt
     3421 ttccggctat tctgatattg caattggctc cgcgcgcatt cttggatttc acattcctga
     3481 aaacttcaac agaccttttc tttctctcac aatgacagag atgtggagaa gatggcatat
     3541 ttccttttcc tcctgggtgc gcgattacgt gtatttcccg ctcggaggtt cgcgcacttc
     3601 acctcctcga gcatacttta atttatttat gactatgttt gtcagcggaa tctggcacgg
     3661 ccccaccatg aactttgtgc tctggggtgt gatgcacgcg gtatggctgg tccttgaaaa
     3721 gctattgttc tctgtggatc gaatcaagaa agcgtatgat tctatccctt ctatcctgaa
     3781 gattgcctat actttcaccg cattttctct tgctctcttc ttctttcgcg cgcggatgct
     3841 ccccggtttt tcgagcgaaa tggaagtagg tttcttcatg ctcaagcgtg ctttcacact
     3901 ggttccaggt gagctggtgc tgcctccagc tggagtggcc gttgggatct tgattctatg
     3961 gattctagaa gcaatcgtgg aaaaaaatga acatgtattt gctccactgt gggaacgtaa
     4021 aggtgtctgc gctgctggag cagtggcttt gctcttcatc agtttctgca tctactctgt
     4081 cactgtgagc gctcagttca tctactttca attttgatta tcgcttgacc ctgaaggctc
     4141 cggtcgcccg ctgttttaat ctccgggagg aaaggggtgc aattcccctg ctgacgcgca
     4201 actgtaaggg ctttgcccga gccagatctt ctccgcagta cggactcgcg aatcggtcca
     4261 ctgctaactc gcagagcgag gagcccccgg attcgggatg cttttggagg gactgtctgc
     4321 gatttctctg ttatttctca atcgccctcg catttcttgc gcctggtctt atgttcgcgc
     4381 aagagatcga ggtaactggc aaaaaaagaa cgttaggcga tcagccggct gcgacttcgg
     4441 agatccctgt atctgattat gccggcagat ttgaaaccct ggaagatatt cttgaaaagg
     4501 aaacctccgt gcgcgtgaag cgatatggag gtccgggctc acagagttcc atttcagttc
     4561 gtggttctaa cgcaaaccag accaacctgt atctggatgg aattccgtta aacaacgcgg
     4621 tgagcgggga agccaatctc gccgattata atctggacgg cgtgggtagc atccaggtat
     4681 ttcgctccgg gcaatcgtct ggtttcagtg gttctgccat tggcggatcc gtgaatctgg
     4741 tgcccgaaag aggcgaaagt ggaacgaggt tgcgccttcg cggaggaacc gaacgcacga
     4801 ttgatgttgc ggcgcgggca ggcggttctc gcaatccaga agaggcggaa aaagaggaaa
     4861 cagaaaaggc taaagtggat ccttccttgc ttgatgcgat gcgagcgcag tggtcggtgt
     4921 ccggccacac gggccgatca gaccaaacat acaaatatcg caacaacaac ggcactcccg
     4981 tattcaacac ttttgatgac catgatgata gaagaaagaa cgcctggtac agggacgacg
     5041 gattcactgg ctatgcaagc gctggcgtgg gaaacacaga tttaaagatt ctagacgatt
     5101 ttacatatcg catgcacgga gtcccgggtc ccggcagtcg gcagacagaa aagacacacc
     5161 gcgaattgac acgcaacacg actggaattg gatcagatac gcgaggcctt ggagtagact
     5221 ggattcgtct ggagaccaga gcgtattata cgtactacag agaatctttc ttcgatcctc
     5281 tccaggagtt ttctacttcc tcccccaatt cgagcggcaa gctcgatgta tatggaattc
     5341 atttgatgcc atctctctat tttccggtcg ctttccagac cattcgcttt ctatttgcgg
     5401 aagagagaga agactatcat caggagattc gaaatcgcct ggaccagaaa acggaaaatg
     5461 taccggcccg atttcgcagg cacacttccc ttcacgcaga agacgaattt tcatttttca
     5521 aaaaaagact gatcattact ccctccgtgc gatacgaact ctatcgggat gagttccctg
     5581 attttcgttt ccgatcttat tctgaatttg gaaatccaga gaagaggcga atcgactata
     5641 ccaatgccag tttattttct tctattgtcc tggcccgaat ttcgcaggca gaatttcgct
     5701 ttcgcgcagg cggtgctaaa gaagagcgca tgcccacgtt tctggagcta tttggcgaga
     5761 gaggaagcgt gatcgggaat tccaatctca aaccggaaaa gtcagagagt tcggagggag
     5821 gtttttccct cgtggcagat cggcaaccta tgtccgggga gttttccatc actctctttc
     5881 gaaagaacat ccgcgacatg attctgtttg ttccaaattc acagttttct ctgagacctg
     5941 agaatgtgga cgcagccaga atcacaggtg tggaggcgtc cgcgaaaatg ttcatcatga
     6001 aacgactgag gctgtatgcc acatacacat accagcgtgc gatcaatatg agcgatgtaa
     6061 gttatctaaa gggaaagtac ctgcctctca ggccattgca cgatctgcat tctggactca
     6121 gcatttacgc gggaaaatgg gaaatgggtg cggaagcaga ttttacgggc gctgtgtttc
     6181 gcgatcgcac aaacgaatac ttgagctatc aggcaggcag gtgggtatac aatgcctatc
     6241 tcactttcgc gcctctgggc cgcggtgaga gagagctgat ttttgggctt gaaatgaaga
     6301 atgtactgga tcaaaggatg gaagatgtga tcgggtatcc gcttcctggg agaacgctgt
     6361 atggaacgat caactacaaa ttctagattc agctttcgca tcgccttacc atggttaggt
     6421 gtgattctcg tatctttcgc ggtgagcgga tgcaaggata ttgagaggcc ccctctctgg
     6481 actttgttct ctgcctccac tggctctgcg cgcgttgggg tggtgactac tgattttgga
     6541 tccaacggta ggttcagtgt aatcacaaca gaagggtttc cgctccccgg actttctcct
     6601 atttattcag atgcggtggc caggtaccag aacaaccagg taaccgtgat caatcgtctg
     6661 gggcgtgaca acattcaagt tcttgagcct gccattggtc ttttgaccgt tcaggaattt
     6721 tctctagggt caggaagcaa tcctcacgat tacattcgtc tttccgatgc gaaaggttac
     6781 gccgcgctct acaatcgagc gtacgttcaa attgtacaac ccgcaacagg tttcctgatc
     6841 ggtacgattt ctctggctgc gtacgcagac gtggacggaa ttccggaggc ggacagcatg
     6901 cttcaagagg gaagttccgt gtatgtggcg ctcgaaaggc tggaccgaaa tcagacaaat
     6961 cttccgccgg taggtgtttc gaaactcgtt gaaatcaatt ctggtagcga tgccattgtg
     7021 gccgcatacg attttccgtc agccaatccg tttggaaaaa tgcgtcgcgt ggttctgggc
     7081 ggcgtgcctc acctggttgt gccatgtccg aatagacttg gatttctttc cgcgatggac
     7141 ggtggagtgg tcgcgttcaa cttgaatact cgcacgtttc gcccggggtt tttgtatgcg
     7201 gaaacaacgg caggaggcga tattctcgat gttgaaattg caagcgatac cgtaggttat
     7261 gcgactgtgc tcgatgcagt gttcaacaaa tctttgcaaa agttcaatcc tactaccggg
     7321 cagaagcttg ctgtgctttc tttttctccg tcgagtgcgg gcacaatatc aggtctgttc
     7381 ctcgcaccga acggttatct ctacgccggg gatgcttctt tttcccaacc gggagttcgc
     7441 atctatgaca ctcaaaggaa cgacgcgctg gtcactcctg tgccggtcag cgtgggtctt
     7501 cgtccgtttg actttgtata tcttccgtga cagttgcaaa aatcgatcga ccgaatagac
     7561 aacgcatccg tccaaagaac atgatgcgcg tagatcgtga ttcccttcgc ttctcgatga
     7621 gtgcatgttt cgcactcgct tatctttttt ctcccggtct ttttgctgaa atcagagtag
     7681 attcgtcatc gcaggttttt tacaaaaatg cgactgcgat gcttggtcgc atgaaggatt
     7741 cgcctgaccc ggaaatacgc aaactctacc aggaagtgtc tagagcatcc gtgactgtcc
     7801 acattcgccc gatcacagat gacagccgca cctggcacgt ggacggagat cggacgcgag
     7861 cccacacgga ggcgcttgac aacctcgggc gcgggagggc gcgaaatcgc cctacgagcg
     7921 cagctattta cctcaccaca gagtcagtgg atcccaccag gacatcctgg aaaggaggtg
     7981 tattgccgca cgagatgacg cacgcgatgg atctggcttc cgggaggtac aacaaagact
     8041 ataagatccg ggaacgccgg gccattgtgt ttcagaatat ctggcgcgct cgcgtcaaca
     8101 aaactctgcg cgaagactac cacggatcct ttgatacgct agactatcag aacgcaaaga
     8161 gggcaggaac gctgggcaag ctcacggaac atattttctc aagctctgat ttgccctcct
     8221 ctcgcgattc aaatgatgaa ggggatgacg atgatgggga cgaggaatga tttcatggag
     8281 cttctcttct aacacgcagg agacattcca tgatcgtttg aatcggtaag aatctcaaag
     8341 gcggcgcgac ttttggaagt aagcatttct gcgtgtgaac tgctggagga taggatctcc
     8401 agatcacacc ttgcgatctt tgaattcaga caatatttga acccacctgg tggattgtaa
     8461 tacttcaagc atacaaaatc ttctctcttt gccgttatgc gacctttgat cttgatttcc
     8521 gcagagcttg catgaaagtc ccagtcaaaa aatccaaagc ttgcgcggcc cattgtcttg
     8581 atcaattggt tcaatctaaa ttcctgtcca cgatgacgaa gcacgatcgg agtgagggcc
     8641 ggagtccaga tgggtccgaa cttcagcttc gcagtggcga gttcgagaaa cgtatcttcg
     8701 ctgtgatcaa agcccgccac ttgaccccat gcatagtgat ctgtatgttt ggaaccccag
     8761 ttgtgattct ggcttcccag ccaggatttg acctgaactt tttgtccgtt gactgtgagt
     8821 gacccatcga acctggccag aggcatgccc accagaacct ttgcttttgg aagtgctgtg
     8881 gaatacatgg actccggaaa atcaaagagt ggctgtgcgt ccgatccaaa gctcagattc
     8941 caggagattg aattttttcc ctgcttcacc tggcctgcta gttcactctg gtttaggaac
     9001 gcgtcggcca ctttcgcacc gaaacgattg cgatcaaatt cgcactctcg gatggggact
     9061 tcttttttca ctgcgatgtg actgttcttt tctccgtcaa agtatatggc ccagagttca
     9121 ccgatcgcag acgcaggatc attctccggg gagaaaacag tatagcgaat ccagaatgcg
     9181 agcgggcgag ttggatggtt tgctctctga aagaagcttt cgtagtgacc ctttttttga
     9241 cccgggatgt atctgctatg attgaaatcc tctcgaatgc ccatgggatg ggaatgcacc
     9301 gggccatcgt gtatgtcaag gtttgcgggg aactcggcgc ggcctcctgt ctgctaaatt
     9361 gactcgacgc ggggcattca tcccagatcg attccatcgt gaacggccgt ctggattttc
     9421 agctcgaagc agaagccagc gatagccacg cgcgcgcagg cagatttcgc acgttgcatg
     9481 gggaaattct cacacctctc ttcatgccag tggggaccaa tgcaaccgtg aaaggtcttc
     9541 gctttgaaga tctggcgcag tcaggctcac agatcctgct tgcaaatacc tatcatttgc
     9601 tgttacgccc gggcccggag gttttccaga cgataggcgg cattcacaat ctaactcact
     9661 ggaggggatc atttttgact gactcggggg gatttcagat cttttccctt tcgcatgctc
     9721 gcagtatgag cgaagagggc gctgcgtttc gtagttacgt ggatggcagg atgattgtgc
     9781 ttacgcctga actgagcatc gcaactcaga aagccatagg aagcgacatc atgatggtgc
     9841 tcgatcagtg cattccgtct acagctgatt ttgaaaccgc aaagcgagcc atggagctca
     9901 cacacagatg ggccagaaga tcattgaatg caagagggga aagtcctcag tctatgttcg
     9961 ggatcgtgca gggcgccatg tattctgaac tccggcgcca gagcgcggag gtattgactc
    10021 agatggagtt tgacggcttt gccattggcg gtcttgccgt aggggaaaca aaagcacaga
    10081 gagaagagtt cacagagttc acagcttctc ttttgccgcg cgatcggccg cgctatctga
    10141 tgggtgtggg cactccgatt gatttgctgg aagctgtgca tcgcggtgtg gatatgtttg
    10201 attgcattat tcctactgct ctttcgcagc aaggagttgc ttatacctcc cgcggcaaaa
    10261 tgcgcttgaa tcgaacggtg tacaagtttg cggaagaaaa actggatccc gcctgcgatt
    10321 gctacacgtg ccgcacttac agcctcgcat atctccatca tttgaaaaaa tcatctgaac
    10381 tcctggggtg gcagcttgtt tctattcaca acttgcattt ctatcatcga cttatggcga
    10441 gtatgcgaac gcacatcctg gccgggactt ttcgaacttt ctatgagaag gcaagggagg
    10501 agctcgtgcg cgaagatgag gagaggccaa aaggtccgcg cccacgagtt cgttctacaa
    10561 aaaactatga atccagagga gccttttctg tgcatcgcac gcgcgaagga tccatgtcga
    10621 tcaaagacag cgcttccggg gaaattatgc atcctggcgc ggatccgggg attgaggccg
    10681 atcgcctgta tgtgaatcag tcaaggctcg cggaccggtt gcgcgaagag ggaaaagaag
    10741 agtccgaact agtcatctgg gatgtgggcc ttggtgcggc aaccaatgcg atggcggccg
    10801 tgagggcgtg ggaactggtt cagtcagaga atgaaaacgt gcgacgactt cgtattgtta
    10861 gttttgaaaa cgatctggac gcattaaacc tggcccttct ccatacaggg aacatcaaac
    10921 acttgagaca ttccgcaccg cgagagatct ctatgaatgg cgtctatcgt tccgagaacg
    10981 gacgactgat ctgggaacta ttgcacggcg attttctaga gcttctgggt tccgcggttg
    11041 ttcccgatct gatctatttt gacccgtttt cctataaaac aaatccgagc ctctggacgc
    11101 tggaaacatt cgcgcgagtc tttcaacgct gtagtgaaca tgacacggaa ctcttttctt
    11161 acagcgcatc gaccagggtt cgttctgctc tcctggctgc aggtttctat gttgcatctg
    11221 gtttgagttc aggcgaaaaa tcggaaacaa ccattgccat gacggaacaa gctttgcttc
    11281 ggcgaaaggg ctcaaccgaa gatgagctgc atttgttgaa tgagcgctgg ctcgaaagat
    11341 ggcagagaag cgatgctcct tatccgttgc tactgaccgt agacgaagag cccggtgtgc
    11401 gcagtttgat tttgaatcac aaacaattca ggtaaaaaaa aagccggccc ctgggggagg
    11461 gccggctcat gggggatgct tattttacct agccaatcgc ttctttgccg cgttcgcctg
    11521 tgcgaatgcg gatgacttct ttgagctctg ttacgaagat cttgccatcg ccaatttttc
    11581 cggtgcgagc accttcaatg acggccttga tcgtcggctc aacgtagtct tcgtttactg
    11641 cgatttcgaa tactaccttg cgaagcagat tcacagtgtg tactgcgccg cggtagttct
    11701 catcgtagcc tttttgctgg ccacagccca tcgcattgag aaccgtcatt ttggttactc
    11761 ctgctttgta cagagcctgt tttacgtcct caactttgtg aggttgaacc attgctgtaa
    11821 tcaatttcat ggtgtttctc ctctacctca atcgttccac atctggaagc cgcggtagcc
    11881 ctcttcacca tgttcactga tgtcgagtcc cagagtttcc acttcttcag acggacggag
    11941 tccaatcgtg aatttgatca gatatgcgat caccgctgtg gcaaccacgc tcagaaccag
    12001 ggtaaagcca attgctttga gctgctcgat ccagagtgtt ccactgttga tgaatgcatc
    12061 aatgttggaa gcaaggttcg cgtttacagc tccagggttt actttgccgt ctgcaaattt
    12121 tgcaggaact gcgaggaagc ctgttaccag agcgcccatg gttccaccta cagcatgcac
    12181 accgaatgta tcgagtgcgt catcatatcc aagccagcct ttcagtttgt agactgcgaa
    12241 gaatggaatg gatccagcca gaagaccgat gatgatagag ccgcttgcgt ttacgaaacc
    12301 gcatgcagga gtgatcacaa ccagaccggc caccgcacct gagcagaagc ccagaacagt
    12361 tggttttctt ttgatgatgt attcgaggaa cggccacacg aagcttgcta ctgctgttgc
    12421 aagagtggtg gtcatgaatg cattggcaga tacaccgtct gctgctacag cagatccagc
    12481 gttgaatcca taccaaccaa cccacagcat tccagtcccc accatagtga gcaccaggtt
    12541 atgcggatcc attgctttgg ttccaaagcc aatgcgctta ccaaggatga tacacagaat
    12601 cagagcagac cagcctgaag acatgtgaac cacagttcca ccggcaaagt caattgcgtg
    12661 aatcttggca gcggagttcc acacaccatt catcataccg ttgattcccc agaccgcgtg
    12721 agcgagtggg aagtaaactg caaacatcca cagtgtaaca aagatgatga tcgcagagaa
    12781 cttcatgcgt tctgcgatcg caccaatgat cagtgcaggc gtgatgattg cgaacatcag
    12841 ttgatacatg gagaatacgt tatgcgacac ccagtatgcg tagtcagtgt ttggaacgct
    12901 gtccacaccg cgcaggaacg catgcttcag actgccgaat aacgttacga ttggagtgag
    12961 tgctcctgtc gcatccgtcg ggctgtcatt ggcaaatacg agactgtagc ctgcagccca
    13021 ccatagaatc gtgacgactc cggcgatgcc aaggcactgt gccataacag agagaatgtt
    13081 cttctggcga gttaagccgc cgtagaacaa tgcgagaccc ggcagggtca tgaaaagaac
    13141 gagagccgag cagaccatca tccagccgtt gtggccagga cctgatcctg gaacgttgga
    13201 tgtccacttt gcagtgccgg ccgctccaac tgcgccgttg ttgatgtagg cttccaggtc
    13261 tgccagacgc tgatctacag ttgggccagc aggtaccgga gccgcagggg tttccgcata
    13321 caatctagca ctaacaagta ccgcgcccag caccaggaaa taagaccatt tcttgaaatc
    13381 cgatttcatc gaatcctcct gagtg
//
LOCUS       DAOQJL010000030        13281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2283_length_13281_cov_4.594057, whole genome shotgun sequence.
ACCESSION   DAOQJL010000030 DAOQJL010000000
VERSION     DAOQJL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2283_length_13281_cov_4.594057"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLG78_02365"
     CDS             <1..546
                     /locus_tag="PLG78_02365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE21978.1"
                     /translation="PIGPAFRYNNYHPLLEGIIIERVTRMPVAKYLEQRIWKPMGAEY
                     DASWSLDQSDGFEKMESGLNARAIDYARFGLLYLHQGKWNGKNILPAEWVRISTTPDS
                     PERPFDTFQGWQKLGGYYAYHFWGLHAPNNQNDFMAMGNYGQIIYVAPRKNIVIVRLG
                     SRDSQTIPFGVLFRELVNQIR"
     gene            554..2536
                     /locus_tag="PLG78_02370"
     CDS             554..2536
                     /locus_tag="PLG78_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNE21979.1"
                     /translation="MLKTIHTDPGLLPFRPRILRRYARIQEVQDRITNGLSLTDFASG
                     YLYFGMHTTAEGWVFREWAPNASEMFLVGDFSQWQIREEFRVHKHGSNWEIQLGHTQI
                     QPGQLYKLWMRWPGGEGFRLPSYGRRMVQDANTKIFTAEVPHREEYKWKVQNFDRGRT
                     PPFIYEVHVGMSSEEEKVSTFNEFRLNILPRIHRLGYNTIQMMAVQEHPYYGSFGYHV
                     SNFFAVSSRFGTPEELKQLIDEAHSLGIAVIMDLVHSHSVKNIDEGLALFDGTPFQYF
                     HDGPRRLHSAWDSLCFNYSKPEVIHFLLSNCKYWLEEYKFDGFRFDGVTSMMYIHHGM
                     GVDFMRYEMYYDGNQDEDAITYLALANKLIHAINPNAITIAEDVSGMPGLASSVENGG
                     YGFDFRLAMGIPDYWIKVIKELPDEKWNVGNIYYEMTNHRAEEKTIAYSESHDQALVG
                     DKTIMFRLTDAEMYFAMHRQAQSLVIDRAMALHKMIRLIVCACSGGGYLNFMGNEFGH
                     PEWIDFPREGNGWSYKYARRQWHLSQDPNLRFRFLELFDHDMIALIRSSDMFTQEFPE
                     VRHQHEGNQTLAFQRGSLYFIFNFNPTQSFEHYELAAWPGNYSIILNTDRDIYGGFAR
                     IDESLTYATTGTEGGPATNSLKLYLPSRTALVLRRI"
     gene            complement(2560..3042)
                     /locus_tag="PLG78_02375"
     CDS             complement(2560..3042)
                     /locus_tag="PLG78_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21980.1"
                     /translation="MRNLKYVIFSFGLSILFSGSLLAQSVAGPGGNYWPLFCKTGPNA
                     KVEVDDTSNLRIFFESSGKAAGNNFAGVTPGTCSYPDRPPRKDELQGYYGEIFAQWHC
                     PALIGTCLIKYDSDSHTYKGNFPWLRDPAGRKFKMWVRKDNGFMQILGDTSPKIEFVN
                     "
     gene            complement(3194..3661)
                     /locus_tag="PLG78_02380"
     CDS             complement(3194..3661)
                     /locus_tag="PLG78_02380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE21981.1"
                     /translation="MADCSGHGCCVCWMMLREVNIDSLYETKSRGFSQAVIVEMRPPA
                     MIQSGVGFISGMVAWDTDRRIPQDIDIGEETTLAMQNVVRLLRAESREVQSIVQVRTF
                     VVNLTPELARTVSRALEVFFTETAHRPASTMVGVTGLAAPEVRVEIEVVFSTH"
     gene            complement(3619..3981)
                     /locus_tag="PLG78_02385"
     CDS             complement(3619..3981)
                     /locus_tag="PLG78_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE21982.1"
                     /translation="MEQGIRDSIEQFVRAGDEQDRDLFLKVTHANFRVVAPNFPAPGS
                     IAFLERDQFASLLQSKQIGGEVRKIDFLGWEPIGPSAATVRTRLTGTKLRFENAFALI
                     KMEDKWQIVQDMAAASAG"
     gene            4143..4574
                     /locus_tag="PLG78_02390"
     CDS             4143..4574
                     /locus_tag="PLG78_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE21983.1"
                     /translation="MDIVSLDGAFAYYINRTYRSLRMDFLDLTRNAGYELTPEQYFIL
                     NKLFHRPGQSQSELAAELNDRANIARGLAILQRKKLIRRSKNSEDKRKFSVQLTKDGE
                     AVLKKLNPVIEARRKVVYSGLTGKDLQTLRHILTKIESNLD"
     gene            4804..5790
                     /locus_tag="PLG78_02395"
     CDS             4804..5790
                     /locus_tag="PLG78_02395"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE21984.1"
                     /translation="MAAQRIPKFAYAVNTGSSTISIYTVNPTTGLLSSAGSSATASGP
                     LGIAVDATGKFVYVANQSANLLSFYTIQSTTGAISAVGTIPAGTGPNSIAVDPATKFA
                     YATNQSSNDVSTYTLNGSTGVLTLSGTIAAGSTPWSIAVDPLGRFAYVPNITSNNVSM
                     YTINAVTGVLSANGSVAAGSTPVTVAVDPSGRFAYTANQGSNDVTMYAINQGTGALTS
                     LGNISAGTGPRAIIVDPSGKFVYAANQSSNDITIYQMNTVTGLLSIFATTPGLSSPMG
                     LSVEPSGNFLYVANNTANNLAMFMIDKVTGTLTLIGTVPAGTNPGAISVASY"
     gene            complement(5851..6648)
                     /locus_tag="PLG78_02400"
     CDS             complement(5851..6648)
                     /locus_tag="PLG78_02400"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF022036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-class carbonic anhydrase"
                     /protein_id="HNE21985.1"
                     /translation="MVAVAVGSLATVALIANDDQPPETGPACENFGPQTPRDIDNKHG
                     ENKQTFQTARDYKEMNLCNIHFHNGAEHKSRAFAMESTAGEHGHGGGLACAISKSLSQ
                     AEMKKPAQEICKGLKPGDTIEVHWVHTSCKVKPGKGLGSCMSASCANPELRVESQVFT
                     LVNDPTAMKFGDFGYGGNIVNGFHQARGIPSNTGIPVQFMGSTTGPKYNDHICSPLQV
                     TWSVRPECAKLDINSLGAWCAQNEFEEDHGHGVRPLIVNPKLFAPIK"
     gene            complement(6971..7240)
                     /locus_tag="PLG78_02405"
     CDS             complement(6971..7240)
                     /locus_tag="PLG78_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21986.1"
                     /translation="MTSIGCTVLRDSHVVLSRGRGRLQVAGLEYPQDKRGQERMQATA
                     ASFQSLEQSLDPEIPLVLLNHHPSDFSFLQNQAVDLILSGHTHGG"
     gene            7417..7803
                     /locus_tag="PLG78_02410"
     CDS             7417..7803
                     /locus_tag="PLG78_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21987.1"
                     /translation="MIKKTILPIVLASAWISISEFVRNTFLLHSYWLDHYKNLGITFP
                     EQPINGAIWGLWSLVFAIVIFILAKKFTLVHTTILSWIIGFGLMWLVIGNLGVLPTGI
                     LILAFPLSVLESFLASLIIKKLSADS"
     gene            complement(7849..8826)
                     /locus_tag="PLG78_02415"
     CDS             complement(7849..8826)
                     /locus_tag="PLG78_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21988.1"
                     /translation="MKQEVKQIFEMVRAGRLTDDQAARLMTEIQSKETRSEKNDGSLG
                     SMLRNIGADVGALSSSVGGLYASSLKENDFSMSRVQAPAGKSFVFEGNSIRMSSLGGL
                     HLEDAEFSGNEIHSSSIQDLVLKSSKFSGTSLTATSMENTELDKANAKSLEISSSSLK
                     KIRIQDSEITQARMVASALKDLSFAENSSWSGVFDKSQISSLTLKKSQMADFEVLASQ
                     VSQVRFEETEGMQVLIRMLKMTGTSFLKCKMSDVLFSGGEKWRKSGFKHVTFEIVSME
                     KVLFSDCNLDDTVIKNVNLKDVRILNANLRGKTIESTEEFLKLCETKAA"
     gene            complement(8832..9221)
                     /locus_tag="PLG78_02420"
     CDS             complement(8832..9221)
                     /locus_tag="PLG78_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNE21989.1"
                     /translation="MSEGIDSSLACPSCRGPLRPRILGCDVCGIKVEGPFVLNEFATL
                     SPEDLHFLRVFVKSEGRVREMQAALGLSYPTVRARLSALKDRLSDDAPAKSVNSPTEE
                     ILSRLSEGKINFEQAMKEIKALPPKKE"
     gene            9464..10126
                     /locus_tag="PLG78_02425"
     CDS             9464..10126
                     /locus_tag="PLG78_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE21990.1"
                     /translation="MMRHMGRLIWTLSLWIVCASAGTCGQYPSSMPWLGLLVAKNNSS
                     ASTSPPIVATPQWKIFVTAGVSSPYIGVSGFDADCMGDTNKPADSSVYKAMIVEGTTR
                     RACSTPMCGGGAAEGIDWVLKPSHRYVRASDSADLFTAGSAAVFSFGTWGNPPDAAAL
                     SFWTGLTASWTNSPSNCLSWANNLGGTFGATGNSSSTNSSVISATALDCTGSYHLLCV
                     EQ"
     assembly_gap    10193..10292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10362..11087
                     /locus_tag="PLG78_02430"
     CDS             10362..11087
                     /locus_tag="PLG78_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21991.1"
                     /translation="METLPNYGLANTITGFATLFSGLLPLCICYLVERHPGRWMFVYW
                     LIVITGIFTITLHGFGETNPMILERWVWSFLDTGSNIVVVWGIVLAVLEDGYSERIRI
                     RARPAATVLMLVGIVWHFFDRMTSGGYLISFGNWGGFRPGQTWLILFSFAATALFYAK
                     RETIPQKARPLLSLTTVIFVAGLLMATARNDTIVFPFLSLHALWHLTAAFGFISLWAF
                     NDVRFRATPRNRDVSRHSNQDLQ"
     gene            complement(11056..11901)
                     /locus_tag="PLG78_02435"
     CDS             complement(11056..11901)
                     /locus_tag="PLG78_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE21992.1"
                     /translation="MDSTNLTPLEMAKAVFLFDFTFWLVRYAVFAALAFAFVYFIARQ
                     KFGSRAIQKTNPSRDRLWKEVGWSVSTLFVFALMAILVVLLMRAGLTRRYNSISDYGW
                     GYYLLSWILFILAHDAYFYWAHRFMHWKPIFRYVHAVHHRSTNPSPLAAFAFHPLEAV
                     VEYAFFIPMIMILPLHISITHTYPSIMLLLNVYAHLGYEFLPRAFASHPIGRWVITAT
                     LHNQHHKKFRVNYGLWFYWWDRWFGTIDPEYETEFRANEGPVRQKLENLQTRHVTVGP
                     GSNAG"
     gene            complement(12017..13123)
                     /locus_tag="PLG78_02440"
     CDS             complement(12017..13123)
                     /locus_tag="PLG78_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE21993.1"
                     /translation="MLRWTSIDFFIAGTLIGATLAIVIGMARLLKHPSTQSDRILGVL
                     FILLSFPQLSIAFERLGLFFIYPRALFPHVPFYVFFGPLAYFYFHQIIEPGFRPVQKK
                     HQLLHLVPFAIVQIALLPLYFLSDAELDRMAHGRCTFQWYLDLIFVGISVYTLIFLAL
                     VLRDFFSLLQNRNGQLPPEYKLTAVFVFLGAVFVSLLVWNQIQRSQMATTLGFLGLGF
                     LPVLMFWGAQRFPQYLDRMKKEASRARYERSRLGGLDSDSLIRRLTELMEIEALYSDE
                     DLDLARLAAILDLTPHKLSEILNDKIGLTFKNYVNGYRVRAACELLVQEPDRAVLSIM
                     GAVGFGSKSSFNAEFVKATGQSPSDYRKNLTRQA"
BASE COUNT         3330 a         3428 c         3144 g         3279 t
ORIGIN      
        1 ccgattggcc cggccttccg atacaacaac taccatccac tgcttgaggg catcatcatc
       61 gaacgagtca ctcgcatgcc ggttgcgaaa tatctggaac aaagaatttg gaaaccgatg
      121 ggtgcagaat acgacgcgtc ctggagtctg gaccagtctg acggctttga aaaaatggaa
      181 agtggactca acgcgcgcgc gatcgactat gcacgatttg gtcttcttta tctgcatcag
      241 ggcaaatgga acggcaagaa cattcttccg gcagaatggg ttcgaatctc tactactcca
      301 gattcaccgg aaagaccgtt tgatacattt cagggctggc agaagctggg tggctactat
      361 gcgtatcatt tctggggctt gcatgccccg aacaaccaga acgatttcat ggctatggga
      421 aactacggac agatcatcta cgtcgctccc agaaaaaaca ttgtgattgt aagactcgga
      481 tcgcgagaca gccagaccat tcccttcgga gtgttgtttc gcgaactggt aaaccagatc
      541 cggtaggcgc gacgtgctca agaccattca cacggatcca ggactgctgc catttagacc
      601 gcgcattctc agacgttatg cgcgcatcca ggaagtgcaa gatcgcatca caaacggtct
      661 ttctctcacc gactttgctt ccggatacct gtactttggg atgcacacca cagcggaagg
      721 atgggtcttt cgagaatggg cgcctaacgc ctcagaaatg tttctggttg gcgatttttc
      781 gcagtggcaa atccgcgaag agttcagagt ccataaacat ggatccaact gggaaatcca
      841 attgggtcac actcaaatcc aaccagggca gctttacaaa ttgtggatgc gatggccggg
      901 cggagaagga tttcgccttc cttcttacgg aagacgcatg gtgcaggatg caaataccaa
      961 aatattcacc gcagaggttc cacaccgcga agagtacaaa tggaaagtgc aaaatttcga
     1021 tcgcggaaga actcctccct ttatttacga agtgcacgtt ggaatgtctt ctgaagaaga
     1081 aaaggtttct accttcaacg aattcagact gaacatactt ccgcgcattc acaggctggg
     1141 ctacaatacg atccagatga tggcggtgca ggaacatcct tactacggat cattcggata
     1201 tcatgtgtct aatttcttcg cagtatccag cagatttgga actccggagg aactcaagca
     1261 attgatcgat gaagcgcatt ctcttggcat cgcagtcatc atggatctgg ttcactctca
     1321 ctctgttaaa aacatagatg aaggactcgc tctgtttgac ggcacaccct ttcaatattt
     1381 tcatgatggc ccgcgcagac tacactcggc ctgggactct ctgtgtttca attattcaaa
     1441 accagaagtc atccatttcc ttttgtccaa ctgcaaatac tggcttgaag agtacaaatt
     1501 tgacgggttt cgcttcgatg gagttacttc gatgatgtac attcatcatg gaatgggtgt
     1561 ggatttcatg cgatacgaaa tgtattacga tggcaatcag gatgaagatg caattacgta
     1621 cctggccctg gcaaacaagc tcatccacgc catcaaccca aacgcaatca caatcgcgga
     1681 agatgtaagc ggtatgcctg gacttgcgtc ctcagtggaa aatggcggct atggattcga
     1741 tttcagattg gccatgggaa ttccagacta ctggatcaaa gtgatcaaag agttgccgga
     1801 cgaaaaatgg aacgttggta acatctacta cgagatgacc aatcacagag cggaagagaa
     1861 aacaatcgca tatagcgaat ctcacgatca ggcactggtg ggagacaaaa ccatcatgtt
     1921 tcgcctgaca gatgctgaaa tgtatttcgc catgcaccgt caggcgcaaa gcctcgtgat
     1981 cgatcgtgcc atggccctcc acaaaatgat tcgcctcatc gtatgcgcat gcagcggcgg
     2041 cgggtatttg aactttatgg gaaacgaatt tggccatcca gaatggatcg acttcccgcg
     2101 cgaaggcaac ggctggtctt acaaatacgc acgccgtcaa tggcatttat cgcaagatcc
     2161 caatttacgt tttcgtttcc tggaactatt cgatcacgac atgatcgccc tgattcgttc
     2221 ttcagacatg ttcactcagg agtttccgga agtacgtcac cagcacgaag gcaaccagac
     2281 tcttgcattt cagagaggat ctctctactt catttttaac ttcaatccaa cgcaatcctt
     2341 cgaacattat gaactggccg cctggcctgg aaactactcc atcattctca acacagaccg
     2401 tgacatctac ggcggctttg ctcgcataga tgaaagtctg acgtatgcaa ccacgggcac
     2461 ggaaggcgga ccggctacaa actcactcaa actctatttg ccaagccgga ccgcgctcgt
     2521 actgcgacgc atttgaacgt tccgtccgcg aagctaaagc taatttacga actcaatctt
     2581 cggagacgtg tcaccgagaa tttgcatgaa accgttgtcc ttgcgaaccc acattttgaa
     2641 tttgcgcccg gctggatctc taagccatgg aaagttccct ttatacgtat gactgtcgga
     2701 gtcgtacttg attaaacagg ttcctatgag cgcggggcaa tgccactgcg cgaaaatttc
     2761 gccgtagtaa ccctgcagtt cgtctttgcg cggcggccga tctggatagc tgcatgtgcc
     2821 cggagtcacg cctgcaaagt tatttccggc agcctttcca gaactttcga agaagatgcg
     2881 aagattggat gtgtcatcca cctctacctt cgcattcgga cccgttttac aaaacagagg
     2941 ccagtagttg ccacctggac cggccacaga ttgtgcgaga agagaaccac tgaacaggat
     3001 gctcaggcca aaagaaaata ttacatattt caagtttctc atccttccac caaaccatgc
     3061 acagatgccg atttcaacag tatttcttca tgaaaaaacg aacatctcac ctactcccgg
     3121 aagagaaccg atctcgccac aacagtggcc catggatccg ttggaacatt ccagagttgc
     3181 gacgaaaact ccatcaatga gttgaaaaga ctacttcgat ttcgactcga acttcaggag
     3241 ccgcaagtcc cgtcactccg accatcgtgg atgcaggacg atgcgcggtt tcagtgaaga
     3301 aaacttcaag tgcacgcgac acggttcgtg ccaactccgg agtcaggttc acaacaaaag
     3361 tgcgaacttg aacgatgctt tgaacctctc ttgattccgc acgaagaagg cgcacaacgt
     3421 tttgcatggc aagggttgtc tcttcaccaa tgtcgatgtc ctgtggaatg cgtcggtctg
     3481 tatcccatgc gaccatacca gaaatgaatc ccacgcccga ctgaatcatt gcaggcggcc
     3541 gcatttctac aatgacggcc tgggaaaaac cacgactctt tgtttcatac aaagagtcaa
     3601 tgttcacttc gcgcaacatc atccagcaga cgcagcagcc atgtcctgaa caatctgcca
     3661 tttatcttcc atcttgatca gtgcgaatgc attctcaaac cgaagctttg ttccggtcag
     3721 acgcgttctt accgttgcgg cagacggacc gatcggctcc cagccaagaa aatcaatctt
     3781 tcgcacttct ccgccaatct gctttgactg cagcaaactt gcgaactgat cgcgctccag
     3841 aaatgcaatg cttcccggag caggaaaatt gggcgcaacc acgcgaaaat ttgcatgggt
     3901 cactttcaaa aagagatccc ggtcttgctc atctcctgcc ctgacaaact gctcaatcga
     3961 atcgcgaatt ccttgttcca tatgtgcctc cattttttac aacattgctc attcgaacat
     4021 aatgttgcat atgcaccaat atagccgcga aatgtatctt atgcaacaaa aaaagattca
     4081 gacgaatgtt ccggcctggt tttttgggtg gcaaacccca gaagcgtcca ccatttggga
     4141 ccgtggacat cgtaagcctg gacggggcgt ttgcttatta catcaatcgc acctatcgat
     4201 ctctccggat ggattttctg gatctcacca gaaatgcggg ttatgaactc acacccgaac
     4261 agtactttat tttgaataag ctatttcaca gacctggaca atcgcaatcg gaactggcgg
     4321 cagaattgaa tgatcgagcg aacatcgcgc ggggccttgc aattcttcag agaaagaagc
     4381 tgatccggag gagcaagaat tctgaggaca aaagaaagtt cagtgtgcag ctcacaaaag
     4441 atggggaagc ggtcttgaag aaactgaatc ccgtcatcga ggcaagacgc aaggttgtct
     4501 attctggact aacgggcaag gatctccaga ctctgagaca catcctgaca aagatcgaat
     4561 cgaacctgga ttagacaagt tagcgtccga gtaggatctc acgcactgcc agacgcattt
     4621 cacgcctgct gcgttgacat agtctttctg gcagttcatc ttttcaggat gaaaggctta
     4681 ttctgctctg gaatgatcgc gatcctcagt cttgcgaagt gcaatttttc cgcggcatgc
     4741 ctgccagcag acccggggtg cagtgctgtg tctttgccat tgtttttgtc cggaagaatc
     4801 actttggccg cccaaagaat cccaaagttt gcatacgcag tcaatacagg tagctccacc
     4861 atctcaatat acactgtgaa tccaacgaca ggattgctct cctcggctgg cagcagcgcc
     4921 acagcctccg gaccactcgg gatcgccgta gatgccacag gcaagttcgt gtatgttgcc
     4981 aatcaatcgg ccaaccttct ctccttctac acgatccagt caaccaccgg ggcgatctct
     5041 gcagttggaa cgattccggc tggcacaggc cccaacagca tcgccgtaga tcctgctacc
     5101 aaattcgctt acgcaaccaa tcagagttcg aacgatgtct caacgtacac tttgaatgga
     5161 tcgaccggcg ttctcactct ttcaggaacg attgccgctg gttcgacgcc ctggtcgatc
     5221 gcagtggatc ctctcggaag atttgcttat gtgccaaaca tcacgtcgaa taacgtttct
     5281 atgtacacga tcaatgcagt cacaggagtt ctttctgcaa acggaagcgt ggccgctgga
     5341 tcgacgccgg tcacagtggc tgtggatcct tccgggagat tcgcgtacac tgccaaccag
     5401 ggttcgaatg atgttaccat gtatgctatc aaccagggaa caggcgcgct gacttcactc
     5461 ggaaacattt cagccggcac aggtcccaga gccatcatcg tagatccatc gggcaaattt
     5521 gtgtacgctg caaatcaatc ctcgaatgac atcacgatct accagatgaa taccgtcacg
     5581 ggactcctgt cgatcttcgc cacgacgccg ggactctcct ctcccatggg tctgagtgta
     5641 gaaccatcgg gtaattttct atacgtggcc aacaacacag ccaacaatct agccatgttt
     5701 atgatcgaca aagtgactgg cacgctcact ctcattggaa cagtgccagc aggaaccaat
     5761 ccgggtgcga tatccgtagc ttcttactag gctgatcttc gccccggcgt ccaattggtc
     5821 agttgattcg cgtaatacct gttagtctct ctacttgata ggagcgaata gtttagggtt
     5881 cacaatgagt ggacgaactc cgtgcccgtg atcttcttca aattcattct gagcgcacca
     5941 tgcacccagg ctgttgatat ccaatttcgc gcactcaggt cgaacgctcc atgtgacctg
     6001 tagcggagaa caaatatgat cattgtattt tggccctgtc gtagaaccca taaactggac
     6061 cggtatgccg gtattgctcg gaatccctct ggcctgatga aagccgttta cgatgttgcc
     6121 cccataacca aaatcgccga acttcattgc tgttgggtca ttgacaagcg tgaagacctg
     6181 ggattcaacc cgcaactcag gattcgcgca agatgcggac atacaggaac ccagaccttt
     6241 accaggtttg accttgcacg aagtgtgcac ccagtgcact tcaatcgtat cgcctggttt
     6301 taggcctttg caaatttcct gcgcaggctt tttcatctcg gcctgggaca gagacttgct
     6361 gattgcgcat gcgagaccgc cgccgtgccc gtgttcgccc gcggtagatt ccattgcgaa
     6421 cgctctggac ttgtgctctg cgccgttgtg aaaatgaata ttgcagaggt tcatctcttt
     6481 gtagtcccgg gctgtttgaa aagtctgctt attttcgccg tgcttgttgt cgatgtcgcg
     6541 cggcgtttgt gggccaaaat tctcacaggc cggccccgtt tccggtggct gatcgtcgtt
     6601 ggcgatcaat gcgactgtcg ccagcgaacc aactgctacg gcgaccatcg ccagcgccca
     6661 catccttgac ttcttcccta tgtgcatcgt aacctcccgc agcattgctg aggtcacgtt
     6721 ttctatcgct cggacttgtc cgtcaaccct tcgattcgct tctcaaacac tgcggcaacg
     6781 atgatggcta acgcaagacg tgaatcgtaa tttcggggga aacaccggag cgcacaggaa
     6841 tggaatgccc tagacccctg tttacataca acttgcaccg gccaccagga gaatcggaaa
     6901 agtacgcacc gctgtaatac ggatatcggt ctggcatcgc gcggagaacg ttccacccgg
     6961 cgatctcgat ctagcctcca tgtgtatgcc cggaaagaat cagatctact gcctgattct
     7021 gaaggaagga aaaatcgctc gggtgatgat tgagcaagac gagcgggatt tccggatcca
     7081 gcgattgctc aagcgattga aatgaagcgg cggtcgcttg catgcgttct tgcccgcgct
     7141 tgtcctgtgg atattcaagc ccggctactt gcagcctgcc tcttccgcgc gaaagcacaa
     7201 catgcgaatc gcgcagaaca gtgcacccga tgcttgtcac cttttgtgtc cattcttcaa
     7261 tgtcgtcgta ccagtcgtga tttccttttt acccctcgac atcctggtct ttcgacattt
     7321 ccctatcctc ggcgcaatcg agcgtgcttc gatgcgctta caactacttg cgcgaggcat
     7381 tcccgacagt ttttttgcca cagggcgaac caaagaatga taaagaaaac catcttgcca
     7441 atcgtgcttg catctgcctg gatctcgatt tctgaattcg tcaggaacac tttcctgctg
     7501 cattcatact ggttggatca ttacaaaaac cttggaatca ccttccccga acaaccaatc
     7561 aacggagcaa tctggggact atggtcactg gtttttgcca ttgtcatttt catccttgcg
     7621 aaaaaattca ccctcgtcca tacaacgatt ctatcctgga tcattgggtt tggtctgatg
     7681 tggctggtga tcggcaatct gggagttcta ccgactggca ttctgattct tgctttcccg
     7741 ctgagtgtac tcgagtcctt cctggccagc ctgatcataa agaagttgtc cgcggattcc
     7801 tgaaaatgcc cgcacattcg tatcagcgcg ggcattctaa attaaatctc aggctgcttt
     7861 tgtttcgcag agcttgagaa attcttccgt gctctcaatc gttttgccac gaaggtttgc
     7921 attcaagatg cgaacatctt tcaggttcac gttcttgatc acagtgtcat ccaggttgca
     7981 atcactgaaa agaaccttct ccatggaaac aatttcaaag gtaacatgct tgaatccgct
     8041 ctttctccat ttctctccgc cggagaaaag tacgtctgac attttgcatt tcaaaaacga
     8101 cgtgcctgtc attttcaaca tgcgaatcag cacctgcatc ccttccgttt cctcaaatcg
     8161 cacttgagaa acttgcgagg ccagcacttc aaaatcggcc atttgtgatt tcttaagcgt
     8221 caggcttgaa atctgagact tgtcaaaaac accactccag gaggaattct ctgcaaagct
     8281 caaatctttc agagcagacg caaccatcct tgcctgtgtg atttcagaat cctgaatacg
     8341 gatttttttc aagctggagc tgctgatctc caggctcttc gcattggcct tgtcgagctc
     8401 cgtattctcc atagaagtag cagtcaggct tgttccgctg aattttgaag atttcaatac
     8461 cagatcctga atactgctgg aatggatttc gtttcctgag aactccgcat cttcaagatg
     8521 caggcctcca agagaagaca tccgaattga atttccttcg aatacaaaag attttcccgc
     8581 gggcgcttga acccgagaca tggaaaaatc attctctttg agagagcttg catagagtcc
     8641 gccgacggaa ctggatagcg ctccaacatc tgccccaata tttcttagca tcgatccaag
     8701 ggaaccatcg ttcttttctg aacgtgtttc ctttgactgt atctcagtca tgagtctagc
     8761 agcctgatca tctgtaaggc ggccggctct gaccatttca aaaatttgtt taacttcctg
     8821 tttcattctg gttactcctt tttcgggggc agagctttga tctctttcat ggcctgttcg
     8881 aaattgattt tgccttcaga caggcgagac aggatttctt ctgttggaga attcacactc
     8941 ttcgcgggcg cgtcatccga cagtcgatcc ttgagcgcac tgaggcgcgc acgaacggtc
     9001 ggataactca ggcccagggc ggcttgcatt tcacgaacac gcccttcgct ttttacgaag
     9061 acgcgcaaga agtgtaaatc ctccggactg agagtggcga actcattcaa aacaaatggg
     9121 ccctccactt tgatgccaca aacgtcacaa ccaaggatcc gcggccgcag aggcccccgg
     9181 caggacgggc aggcaagact cgaatcgatc ccttcgctca ttttaccctc aacttaagac
     9241 tcaatttcgg cgcaatattt taatatgtca aatatatttt taatatttta aaacaagact
     9301 tgaatgactg aaaagagtgt tccttttttt gcggaaatgt gacataatcc ggtctatgcc
     9361 cgcctcccgg gccgggtggg cagtctggtg accacgacgc ggaaggaaga gcgtttcgcg
     9421 cccgaggttt tgacaaactc agggttttgt tcagaatcaa gacatgatgc ggcacatggg
     9481 gagattgatt tggactcttt cgctctggat agtttgcgcg agcgccggta catgcggcca
     9541 gtatccttct tccatgccat ggctcggcct tctcgtggct aaaaacaatt cgtctgcgag
     9601 tacatcgccg cctatcgttg ccacgccaca gtggaagata tttgttacgg cgggcgtttc
     9661 ctctccttac ataggagtct ccggattcga cgcagattgc atgggggata cgaacaaacc
     9721 tgcagacagt tcggtttaca aggcgatgat cgtcgaaggc accacgcgcc gtgcctgctc
     9781 aacgccgatg tgcggcggcg gcgctgcgga gggaattgac tgggttctta agccttcgca
     9841 ccgctatgtg cgcgcatccg attccgcgga tttgttcact gcaggatctg cggctgtctt
     9901 ttcgttcggc acctggggca atcctccgga tgcggccgcc ctctccttct ggacgggtct
     9961 gacggcttcc tggacgaatt caccatccaa ttgcttgagc tgggcgaaca acctgggtgg
    10021 tacgttcggc gccacgggca attcaagttc tacgaattct tccgtgattt ccgcgacggc
    10081 cctggactgc accggaagct atcatctgct ctgcgtggaa caataagcca ccaccctttt
    10141 ttttaccgca aaattcgacc gggattcaga gattggtcgc acgtcgactt ttnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttttta ccgcaaaatt cgaccgggat
    10321 tcagagattg gtcgcacgtc gacttttacc aatcgaatca aatggaaaca cttccgaact
    10381 acggcctggc aaacacaatc acaggctttg ccacactgtt ttctggtctt ttgccgctct
    10441 gcatctgcta tctcgtagag cgtcatcccg gacgatggat gttcgtctac tggctgattg
    10501 tcatcaccgg aattttcacg attacactgc atggcttcgg agaaacaaac cccatgattc
    10561 ttgaaagatg ggtgtggtct tttctggaca caggatcaaa tatcgttgtt gtatggggga
    10621 tcgttcttgc tgtgctcgag gatggttaca gtgaacggat cagaattcgg gcacggcctg
    10681 cggcgactgt gttgatgctc gtcgggattg tgtggcattt ctttgatcga atgacgtcag
    10741 gcggctacct cattagtttt ggcaattggg gcgggtttcg cccggggcaa acctggttga
    10801 tcttgttctc tttcgcggca actgcacttt tctatgcgaa gagagaaacc attcctcaga
    10861 aagcgcgacc cctgttgagt ctgaccactg tgatctttgt tgcgggcctt ttgatggcca
    10921 cagcccgcaa cgatacgatt gttttcccct tcctgagcct gcatgcactg tggcacttga
    10981 ccgcagcatt cggatttatc tccctctggg cgttcaatga cgttcgcttt cgcgcgaccc
    11041 ctcggaatag agacgttagc cggcattcga accaggacct acagtaacat gtctcgtctg
    11101 caaattctct agtttctgac ggaccggtcc ttcgtttgcg cgaaactccg tttcgtattc
    11161 aggatcgatt gtaccaaacc agcggtccca ccagtaaaac cacagaccgt aattcactcg
    11221 aaacttcttg tgatgttgat tgtgcagagt cgcggtgata acccagcgtc caattgggtg
    11281 actggcaaac gctcgcggta agaattcgta tccaaggtgc gcatagacat taagcaacaa
    11341 catgatcgaa gggtatgtgt gggtaatgct aatgtgcaag ggcaagatca taatcattgg
    11401 aataaagaat gcatactcaa ccactgcctc gagcggatga aacgcaaaag cggccagtgg
    11461 cgaaggattc gtcgacctgt gatgaacagc atgcacatag cgaaagatag gcttccagtg
    11521 cataaagcga tgagcccaat aaaagtaggc atcgtgtgcc aggataaaaa gaatccagga
    11581 gagtaagtag tacccccagc cgtaatcaga gatgctgttg tatcgtctgg ttagacccgc
    11641 tcgcatcagt agaactacaa ggatcgccat gagcgcaaac acgaacaaag tgctgacgga
    11701 ccagcctact tctttccaca gtcgatccct ggatggattt gttttctgaa tggcacgtga
    11761 tccgaatttt tgcctcgcaa taaaatacac gaatgcaaac gcaagcgcgg cgaatactgc
    11821 atagcgcacc aaccagaaag tgaaatcaaa cagaaagacg gccttcgcca tttccagggg
    11881 agtgagattt gttgagtcca aagtaagtcc tcctttcatt tggatggact cagtatcgca
    11941 cgcttttgcg tgcgcgtcgc cctcgcgtat acgcaaggag ctcctggatt atacgcacgg
    12001 accaggcatg atgggcttat gcttgcctgg ttaaattctt cctataatca gatggagatt
    12061 gtcccgttgc cttgacgaat tctgcattga aagacgattt agatccgaac ccaaccgccc
    12121 ccatgatcga aagcaccgcg cgatcgggct cctggaccag gagttcgcat gcagcgcgaa
    12181 ccctgtaccc atttacgtag ttcttgaatg tcagaccaat cttatcgttt aggatttcag
    12241 aaagcttgtg gggagtcaga tccaggatag cggccagacg agcgagatcc agatcttcat
    12301 ctgagtagag tgcttcgatt tccataagtt ccgttaaacg acgaatcaaa gaatctgaat
    12361 ccaggccccc gagtctcgaa cgttcgtatc tcgctctcga ggcttccttc ttcattcgat
    12421 ccagatactg aggaaaacgc tgtgctcccc agaacatgag cacgggcaag aatccaagtc
    12481 ccaagaatcc cagggtggtt gccatttgac tcctctggat ctgattccag acgagcagtg
    12541 aaacgaacac agctcccagg aaaacaaaca ctgctgttaa tttgtactcc ggaggcaact
    12601 gtccattcct gttttgaagg agcgaaaaga aatcgcgcaa caccagtgcc agaaagatca
    12661 aagtataaac tgaaatgcca acaaagatga gatccagata ccattgaaac gtgcacctgc
    12721 cgtgagccat cctgtccagt tccgcgtcgg aaagaaagta gagcggcaaa agtgcaattt
    12781 gcacgatcgc aaagggaacc aaatgcaata gctgatgctt tttttgaaca gggcgaaacc
    12841 cgggctcaat gatttgatga aaatagaaat acgcaagtgg accgaagaac acataaaagg
    12901 gtacgtgagg aaaaagagcg cgcggataaa tgaaaaacaa gcccaatcgt tcgaatgcaa
    12961 tggaaagttg cggaaaagag aggagaatga acaagacacc gagaattcga tccgattgag
    13021 tcgacggatg tttcagaagg cgcgccatcc cgattacgat cgcgagggtc gcgccaatca
    13081 gagtgccggc gataaagaaa tctatactgg tccagcgaag catttggaat tccggcgatc
    13141 gaggcttgag cgctctgtcg ctcaaatgtt tactctggta agaaatcctc gcgtgcatag
    13201 taggattctc agctatgtaa aagaaaaagg ctattttgaa agccttctag ttccctgtga
    13261 tcaagcattg gccgcctaac g
//
LOCUS       DAOQJL010000031        13194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2303_length_13194_cov_4.154426, whole genome shotgun sequence.
ACCESSION   DAOQJL010000031 DAOQJL010000000
VERSION     DAOQJL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_13194_cov_4.154426"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PLG78_02445"
     CDS             complement(<1..1329)
                     /locus_tag="PLG78_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21994.1"
                     /translation="MKMSPGAFAFIFSIFSAAGLGAQAFEGRLAVGSFHNCAIVENGK
                     VVCWGLGDTGQIGDGKLVNALTPRFVSGITNAKSLAAGEHWTCALLADSSVKCWGFIA
                     APWAPASEKSKTRPCAWGSHTDPCTPVPVSVPPAQGITSIMGMATMTYGILPDGKIVV
                     WTGNDGTANLLKDGKNIQLSGRSSMVIDWIPDAKEVATIYVSTGDMPSHMCAVTKKGR
                     IYCHGATPAVYGPRKPICTAEPRIKPVCQIDFQGEKISVTGEDAPRMCKYAASLDPQM
                     MKSYEWYCQGQTCTYREVPLQKATHLWYLIMANGKEIKSHVTGYAEIFKSTEAEKSKC
                     RDDFYTVELDATPMEGLTDVRRVSLYSDSSAFGITNKDEVYYWGSGREDRDILPDVVP
                     ESSGKPIPPVKIPQFTGAISIRHMSWNGCALFKTGIVKCWGVAGDEGRLGN"
     gene            complement(1443..1703)
                     /locus_tag="PLG78_02450"
     CDS             complement(1443..1703)
                     /locus_tag="PLG78_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21995.1"
                     /translation="MKHKVLMAAAIAGLALGAVAAHEGEDHSKDKGKCFGINSCTGKG
                     QCATKEHTCAGMNTCKGKGWIKLTKAECEKKGGKFDASKPDM"
     gene            complement(1728..2279)
                     /locus_tag="PLG78_02455"
     CDS             complement(1728..2279)
                     /locus_tag="PLG78_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE21996.1"
                     /translation="MRKLCVAVVTLILALCAIPSAVFAHEGHPHGVVFGGFSNLHPLI
                     VHLPITMLLVAPILQILSVFQKGKLPQWTTLAFVLLGAVGAYLSGEVFHPDTTDLGDV
                     ANKILESHEQWADRSQILSYIAVIPAALALFIARFEKHMKVIAFAVLLAAGACIVVAG
                     HEGAKLVHIHGIGAEGNYIKDSK"
     gene            complement(2324..4438)
                     /locus_tag="PLG78_02460"
     CDS             complement(2324..4438)
                     /locus_tag="PLG78_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE21997.1"
                     /translation="MARLFAAMVLSLPATLWATPLLRTAEPVIPEMQILEDRANLTPE
                     KAAASSFTVAPEHLSFGYTDSVTWLKFDLENQTSEPHWLIYISYPLLDRVTLYTREDG
                     SLRAHESGQLVSMSAKDYASRYIVFRITIEPGSRQSYLLRVQSADSVVIPLQVMTFES
                     LASREHNEQIAVGVFYGIVILLILYNLLIFFYAKESSYLFYVYYVTSYLLMQLSENGI
                     LGEMLLPEVPWFANQLLAFLVYHVIFAASAFSYRFLRAERFKALSYILRVFGFLGVVG
                     MCATFFVPYMIAVMPSVIIVMSFGPIVVPLAILDWRRGNRAAGHFVLAWSAILAGGFV
                     YGLKAFGILPDMFITRYGAMLGFCVQSVLLSLGLADGIKVLNLELSQLKTSLELRTRY
                     LENIISHAKEASSSLIDANQEETALVQGLVSMADSQADLTHQLSSAFEELSASSESIH
                     GITTRLLENWQETRTTISALSSVQETVLSSGRAVLENVLEISQSSDESGKRLDQMQDQ
                     MKVIQEGGRAISRFAKTISDITDKTNLLALNAAIEAARAGEHGRGFAVVADEIAKLAE
                     ATQKSSKEIFKQIEGIQKDIEGGMDSASAMREALGAVFTRLQKVRASIDDVNASMSAQ
                     ENSMQGLLRQTAQLENMSARIVETTQEQKNSTQSSQNSVVEIAQMAADLSESSKKILD
                     LGSRAAQKAEDLNASIQQVQND"
     gene            complement(4496..5278)
                     /locus_tag="PLG78_02465"
     CDS             complement(4496..5278)
                     /locus_tag="PLG78_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE21998.1"
                     /translation="MKNTDPAGALLRFQEVGQGHPLIILHGLFGSGRNWGSVARSLSE
                     KFRVFSLDQRNHGDSPQFPSHTLDDLANDLGEWIESHMKDVPVLLGHSMGGIAAMEYT
                     LRHPDQVRALIVVDVAPRPYEPRHSGEFQAMEADLSSCQTRSDVDRVMAPFVPDLTVR
                     QFLQTNLIRKDHGFGWRIPVETLKKASFLQGFEPGRSWNGPTLFITGSQSDYTSESDH
                     TVMRQCFPEAYIETIEGAGHWPHFSHLVPFLGLIKDFLGRAL"
     gene            complement(5265..6113)
                     /locus_tag="PLG78_02470"
     CDS             complement(5265..6113)
                     /locus_tag="PLG78_02470"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE21999.1"
                     /translation="MYSHQESTFKSSKDDFPIYYQKWTPARTRRIVVIQHGFGEHSGR
                     YQNIRDAFEGTDTAFYGLDSRGHGKTGGKRGHVPQFQLYVDDLADLIQLVRKENMNDE
                     VYLLGHSLGGVIVLQYALQGTNQSNLSALIVSSPGLIIEMDASKQVKKVMATLFASVA
                     PAATLEAGLDLKYLSHDQAVVDAYKADPLVHGKISFQMGSNLFHLGKTIFEKANHLTI
                     PVYVFQGTEDKIVSPESARQLYKSLTTEDRTLNMYEGLYHETMNETLADRNKVLGDLR
                     AWVEKH"
     gene            6209..7105
                     /locus_tag="PLG78_02475"
     CDS             6209..7105
                     /locus_tag="PLG78_02475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE22000.1"
                     /translation="MKWIGLLIIGLHTFCARPPEVDSHVAEIAREAQQLKAEIAETEE
                     CKSGCIFLSAWDFDGTILKGDMAEGLKEGERVVYPGLQQLAVDQGWSRTYRPGSFQKF
                     LADRSVIEEKEGHLAALSMGLKIFRGVPVMRLESLASSHFEKVLSRYYFRSSISILEN
                     TKKAGIVPCILSASPHVFARSAAATVGVPRENIYAMETEIVRGFMTDKIVEPVPYAKG
                     KAQRLEMIVENLQKTTGRRVFVVAAFGNSFRSDSYFLKWTLDQKLPRGHALAVMINGG
                     PDAGEFRGLFREVSQIATVSGR"
     gene            7181..8296
                     /locus_tag="PLG78_02480"
     CDS             7181..8296
                     /locus_tag="PLG78_02480"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNE22001.1"
                     /translation="MRVEQIQPGDVSTLTGAHLVLVFGNLDALKLPGFRASLRSKYPN
                     AHIVGCSTAGEIAGDHVRDETVHLTAVEFSNTRVQSAFVHLPSHGSSYSAARSLAMAI
                     PSRDLRHAIVFSEGIRINGSELSRGLKDGLPPNVSVTGGLAADGDRFGQTWVLSNDAE
                     GPDHAVIVGLYGPSLRVGFGSLGGWDSFGPERRITSSSGNRLTGLDGESALALYKKYL
                     GEHASSLPSSALLFPLAIRAEGDDRFLVRTILGIDESDQSLIFAGDMPQGWLARLMKA
                     NFDRLIDGAEGAATTASRRIGNTSPELALLISCVGRKLVLKQRVEEEIEAVRSVVGPG
                     AAITGFYSYGEIAPVSDGSPCELHNQTMTITTLAEGE"
     gene            8293..10128
                     /locus_tag="PLG78_02485"
     CDS             8293..10128
                     /locus_tag="PLG78_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE22002.1"
                     /translation="MSDSLHNLLQRQLRKYPLPQIDGELIAAIDAAYRQFDRDREMLE
                     RSLDLTSHELLVKNEQLSRSVTLLHTALESTADGILVTDLNGRVIARNGRLQAMWTLP
                     LATIEAGEDAIIQHFQGMVIHPGSSLNEPVQSGSEVRELWNLKDGRFFDCCIRPHLID
                     NRAVGRVWNFRDVTEQMNARDDLVHAAFHDPLTGLPNRSLFMNSLDRLITLALPDARM
                     FAVFFLDLDRFKVINDGLGHIAGDILLVEIARKLRAMIPPHCMVARLGGDEFTVLAEN
                     AADEREIIHIAETILREIKSPMMVDGHEVYTTVSIGIALSTGDYRRPEEMIRDADTAM
                     YQAKKQGKSRYQIFQAQMRARALEAVRVEADLRRALPGREFELFFQPIVRAQERQVIG
                     FEALIRWNHPERGYISPLDFISVAEETGLIVQMGEWVVREACKAAKRSLGGRYVAVNL
                     SAVQLQQPHFVETIRSALIGAALPPELLLLELTESVLMESDPAALQALGILRDMGVSL
                     AIDDFGTGYSSLSYLKKFPVNVLKIDRSFVRETPADTSSCEITRVILSMCRILGLRVV
                     AEGVENDAQLQFLSGEGCENIQGFFFSRPVPEKNIDSVIRSISGV"
     gene            10129..10920
                     /locus_tag="PLG78_02490"
     CDS             10129..10920
                     /locus_tag="PLG78_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HNE22003.1"
                     /translation="MLKKILPLLTLLFGALVASGAGFYLVRVRETALAEYIKQAHQIE
                     REFVTRTPIYEDFATPDMEMTLRKYLLMDHLRVAEKAGVGAVATDEEVQKLVEEGKLV
                     PARPDEDADFHFYNVPKEGRFLTPRAREGLELIAQRFQKNIEKRYQKHPPVRIAVSSV
                     LRTTGYQKDLQKKNPNASFASSHSYGVSFDLFYDDYYVKLPPVSPGIPDELRTRLGYL
                     LGDALMRQFRAILMETLIQLQEEGEIYAILEKNQRCYHVTVLEKK"
     gene            10956..12794
                     /locus_tag="PLG78_02495"
     CDS             10956..12794
                     /locus_tag="PLG78_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE22004.1"
                     /translation="MDRLTEQLEELQRGRQLIVYALVFFAVMFVAEAIAGFGFGRWFS
                     HRLESERKSEFSRAFDNYLHQRWNFHSGAVTGYAWWAEPWNLARTGRWAEAKALFEGD
                     PTLQGSFDYFAVYSQSDTPRFEFPGAGNTPLVIPPDIVRFLVESRSMQRGETNLILRI
                     GDKPYLVTAAAMADAQGRPQIPGICVFAFSLDRFLISAREILPATLELSEDGSGFLSV
                     PLVASMRSTDAPLYVRITPNTLMEDLIRNILLAFLAFQIVLSILLFAVISPRYVRERT
                     MRLEKMVRASRILNRELELRVDELTAARTEIQKSEKKYRDLVESSREIIFSIDSKGNI
                     TTINQAVSSLLGFQPESLIGKSILDLIYVSPDRSMNFRREFISEQIESVIQNQKVASF
                     KTEFVTRYNEPRELETTLEYVPLDGGYAIFGKAASMVEDMLATYCERESMKFVIGNYL
                     TLSEQISQRVTNNLGLHCDAGTTLQIRICVSEMIINGIEHGNLGITYEEKTNATEDGS
                     YLALLRRRQFEAPYSSRKLKVVYSLNPRRLLIVVQDEGAGFNHTQILKDNSMEANKKM
                     HLHGRGITMAKSVFDSMRYNEKGNRVALLKRFRNQTPPTSPGSTIV"
     gene            complement(12755..>13194)
                     /locus_tag="PLG78_02500"
     CDS             complement(12755..>13194)
                     /locus_tag="PLG78_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22005.1"
                     /translation="HLTSIIELLEVGYESPELPVLTPSELAAANRECIKAYDFIVIKR
                     DYLVSRPTPERFYEWLQRFESLALGRTPRALGGEPHPLPRRAVITFAKPFTLGEHYDA
                     FQKGKGEYVEQLTNEIRAQMQALLDASASLTYTIVDPGDVGGV"
BASE COUNT         3088 a         3208 c         3501 g         3397 t
ORIGIN      
        1 gtttccgaga cgtccttcat ctcccgcgac tccccagcat ttcacgattc cggttttgaa
       61 tagggcacat ccgttccagg acatgtgccg aatggatata gctcccgtaa attgtggaat
      121 cttgacagga ggaatgggtt taccgctgga ttctggcacc acgtccggca gtatatctct
      181 gtcttcgcga ccgctacccc agtagtagac ttcgtctttg tttgtgattc caaacgcgct
      241 gctgtcagag tagagggata ctcgcctaac atcggttagt ccttccatgg gcgttgcatc
      301 cagctccact gtgtaaaaat catccctgca tttggatttt tctgcttccg tggatttgaa
      361 aatctccgca taccctgtta catgtgattt gatttcttta ccgtttgcca tgatgagata
      421 ccaaagatgc gttgctttct gcagagggac ctcgcggtaa gtgcaggttt gtccctggca
      481 ataccattcg tacgatttca tcatttgcgg atcgagagat gcagcgtact tgcacatgcg
      541 cggtgcgtct tcgcctgtga ctgaaatctt ctctccctga aagtctatct ggcatactgg
      601 tttaatcctg ggttctgccg tgcagattgg ttttcgcggg ccatacacag ccggtgtcgc
      661 gccatggcag tagatccggc ctttctttgt tacggcacac atatgcgaag gcatatcgcc
      721 tgtgctcacg tagatcgtgg ctacttcttt tgcatcggga atccagtcaa tgaccatgga
      781 actgcgcccg ctcaattgaa tattttttcc gtctttgagc agattggctg ttccatcgtt
      841 gccggtccac actacgatct ttccgtcagg tagaatcccg tacgtcatcg tggccattcc
      901 cataatcgat gtaattcctt gagctggagg aacagaaacg ggaacaggcg tacatggatc
      961 tgtatggctg ccccatgcgc atggccttgt ctttgatttc tcggatgctg gcgcccaggg
     1021 cgctgcgatg aatccccagc acttgacgga tgaatccgca agaagggcgc acgtccagtg
     1081 ttcgcctgct gcgagactct ttgcatttgt aattccactc acgaatcgag gagtcagtgc
     1141 gttgaccagt ttaccatctc cgatttgacc tgtgtccccg agcccccagc aaaccacttt
     1201 gccattttca acgatggcgc agttgtgaaa gctgcctacc gcaagtctcc cttcaaaggc
     1261 ctgagcacca agccccgccg cagaaaaaat ggagaagata aatgcaaagg ctcccgggct
     1321 cattttcatg aggccaactc agaagcgaat ggttccccgg atcaagttgt tttccggcga
     1381 tgcgaattgc gaccaggcat gccgccgaca gggtcaaacc tggtcaggtg agttcttttt
     1441 gattacatgt caggtttgga cgcgtcgaat ttgccgcctt tcttttcgca ctccgctttt
     1501 gtgagtttga tccagccctt tcccttgcac gtattcattc cggcgcaagt gtgttccttt
     1561 gtggcgcatt gacctttgcc tgtgcaggag ttgatgccaa agcattttcc tttgtctttg
     1621 gaatgatctt ctccttcatg tgcggctacc gcgccgagtg cgagtcctgc aatggccgcg
     1681 gccatcaata ctttatgttt cacaactgtt ctcctgaacg atttggttta cttactatcc
     1741 ttgatataat tgccttctgc accgatccca tggatgtgaa ccagtttggc tccttcgtga
     1801 ccggccacta caatgcacgc acccgctgcc agtaggactg cgaacgcgat aaccttcata
     1861 tgcttttcaa aacgtgcgat gaacagggcg agtgcggcgg ggatgactgc aatgtaagaa
     1921 aggatttgag agcgatccgc ccactgctcg tggctttcga ggatcttatt ggcaacgtct
     1981 cccagatctg ttgtgtcggg gtggaagact tctccgctta gataggcacc cacggctccg
     2041 agtaatacga atgcgagagt ggtccattgc ggtaatttcc ctttctgaaa aacggaaaga
     2101 atctgaagga tgggtgcaac cagaagcatt gtgatgggca gatgaacgat caacggatgg
     2161 agattggaga aaccgccgaa cactacgccg tgcgggtgac cttcatgcgc gaatacagca
     2221 gatggaatgg cgcagagcgc aaggatcaag gtcacaactg cgacgcagag ttttctcatg
     2281 tgatcgcaat atctatctac aaaaggggat cgtccagcgt aaattagtcg ttttggacct
     2341 gctggatgct cgcattgaga tcttccgctt tctgtgcagc gcgcgatccc aggtccagga
     2401 tctttttgct ggattcggaa aggtccgctg ccatttgcgc gatttctacc actgaatttt
     2461 gactggattg agtggagttt ttctgctcct gagttgtttc tacaattcgc gctgacatgt
     2521 tctccagctg tgctgtctgg cggagaaggc cctgcatgct attctcctgc gcagacatag
     2581 atgcatttac gtcgtctatg gaagcgcgaa ccttctgaag tctggtgaag acagcgccga
     2641 gcgcttctct catggcgctt gcagaatcca ttcctccctc tatgtctttt tgtattcctt
     2701 ctatttgttt aaagatttct tttgaacttt tctgtgttgc ttccgcgagt ttggcgattt
     2761 cgtctgcgac gacggcgaat ccgcgtccat gttctccggc gcgcgccgct tcaatagccg
     2821 cattgagtgc aagcaggttt gtcttatctg ttatgtcgct gatggtttta gcaaagcgcg
     2881 aaatggcacg cccgccttcc tgaattactt tcatttgatc ctgcatttga tctaaacgct
     2941 ttcccgactc atccgacgac tgcgaaatct caaggacgtt ttcaagaacg gctcttccgg
     3001 acgagagcac ggtttcctgc acggaagata gcgctgaaat ggtcgtgcgc gtttcctgcc
     3061 agttttcgag taatctggtt gtgattccgt gaatggactc tgaagatgca gaaagttctt
     3121 cgaatgcaga cgatagttga tgagtgagat ccgcctggct atctgccatg ctcacaagac
     3181 cttgaacgag tgccgtttcc tcctgattgg cgtctatcag tgaagaagac gcttccttcg
     3241 catgcgaaat gatattctct aaataacgag tgcgaagttc cagacttgtt ttgagctgac
     3301 tgagttccag gttcagtact ttgattccgt ctgccagacc gagagagaga agaacggatt
     3361 gaacgcagaa tccaagcatg gcgccgtatc gtgtgatgaa catgtccgga agaatgccga
     3421 aggctttcag tccgtataca aatccgccgg cgagtatggc agaccaggcc aggacaaaat
     3481 gaccggctgc gcgatttccg cgcctccagt ccaggatcgc cagaggaaca acgattgggc
     3541 caaaagacat aactatgatt acagacggca tcaccgcgat catataggga acgaagaacg
     3601 tcgcgcacat tccaaccacg ccgaggaaac cgaaaacccg gagaatgtag ctgagagcct
     3661 tgaatcgttc tgcgcgaaga aatctatagg agaacgcaga tgctgcgaat attacatggt
     3721 agaccaggaa tgcgagcagc tggtttgcaa accacggcac ttcgggtaga agcatctccc
     3781 cgaggattcc attttcggaa agctgcatca ataaatagga tgttacgtag tacacataaa
     3841 ataagtagct tgattctttt gcgtaaaaga atatgagtag attataaaga atcaaaagta
     3901 ttacaatccc gtagaaaacg cccaccgcaa tttgttcatt gtgttccctg gaggccaggg
     3961 attcgaaggt catcacctgg agaggaatga ccacagaatc agcagactgc actcgaagga
     4021 ggtaggattg tcttgatcct ggttcaattg taatgcgaaa cacaatgtat ctggatgcgt
     4081 agtctttcgc agacatagaa accagctgac ccgattcatg ggctcgcagt gaaccgtctt
     4141 cgcgcgtgta gagagtgacg cgatccagaa gtgggtagct gatataaatg agccagtgag
     4201 gttcactggt ttgattttcc aggtcaaact tgagccaggt gacagagtct gtgtacccga
     4261 atgatagatg ttctggtgcc acggtgaagc tagacgcggc tgctttctca ggcgtgaggt
     4321 ttgctctgtc ttccaggatc tgcatttctg gaatcacagg ttctgccgtc cgtagtagcg
     4381 gagtggccca gagagtggcg ggcaaggaca gaaccatggc tgcaaacagg cgggccatac
     4441 ttatgatcat cggtccatgg acgcggtgaa gtcaaactcg attgtgcaat tcggatcaga
     4501 gggcgcgccc gaggaaatct ttgatcagac ccagaaacgg aaccaaatga gagaaatgcg
     4561 gccaatggcc cgcgccttcg atcgtttcaa tgtatgcttc tggaaagcat tgccgcatga
     4621 cggtatgatc cgattcagaa gtgtagtctg actgcgatcc agtgatgaaa agggtgggcc
     4681 cattccacga tcgtcctggc tcaaaaccct ggagaaagga agctttcttt agcgtttcta
     4741 cggggatgcg ccagccgaag ccatgatctt tgcgaatcag attggtttga agaaattggc
     4801 gaacagtcaa gtctggaaca aacggtgcca tcacgcggtc cacatccgat ctggtctggc
     4861 aggagcttag atctgcttcc attgcctgga attcgccgga atgtctgggc tcgtagggtc
     4921 ttggcgccac atctactacg atcagtgccc gcacctggtc tggatgtcgg agggtgtatt
     4981 ccatcgcggc gattcctccc atagaatgcc ccagtagaac cggtacatct ttcatatggg
     5041 actcgatcca ttctccaaga tcatttgcaa gatcgtctaa cgtgtgcgaa ggaaactgcg
     5101 gcgaatctcc atggtttctc tggtctaatg aaaagacgcg aaacttctct gaaagggacc
     5161 gcgcgacgga gccccagttt cttcctgagc caaaaagccc gtgcagaatg atgagagggt
     5221 gaccctggcc gacttcctga aaacgcagca gggcccccgc aggatcagtg tttttcaacc
     5281 caggcgcgca aatccccgag tactttgttt ctatctgcga gagtctcatt cattgtttcg
     5341 tgatagagac cttcgtacat gttgagagtg cgatcttccg tagtgagaga tttgtagagc
     5401 tgtcttgcgc tctccggaga tacgatcttg tcttctgtac cctgaaacac gtatacgggg
     5461 atggtgaggt gatttgcttt ttcgaaaatg gttttgccca ggtggaagag gttgcttccc
     5521 atctggaaag aaatctttcc gtgaacgagc ggatccgctt tgtacgcatc cacaaccgcc
     5581 tggtcatgcg acagatactt tagatccagt cctgcttcca gagtggccgc gggtgccacg
     5641 gatgcgaaca aagtggccat taccttcttc acttgcttgg atgcatccat ctctatgatg
     5701 aggcccgggg aacttacaat gagtgcggac aagttggact ggttggttcc ctgcagagcg
     5761 tactgcaaaa cgatgacacc gccgagagaa tgcccgagca ggtagacttc atcgttcatg
     5821 ttctctttgc gaacgagttg aatcaagtca gcaagatcat ctacgtaaag ctggaactga
     5881 ggaacgtgtc ctcgctttcc acctgtcttt ccatgcccgc gcgaatcgag tccgtagaaa
     5941 gcagtgtccg tcccttcgaa tgcatctcga atgttttgat atctgccgga gtgttctccg
     6001 aagccatgtt gaatcacaac gatgcggcgg gtgcgcgcgg gggtccactt ctggtagtaa
     6061 atagggaaat catcttttga agacttgaat gtggattcct gatgagaata catgggggac
     6121 ggtctgacag ccgggccagg ggatggcaag caagaaggag aatttttctt cgattgactg
     6181 cgtccttcct gttacgcgga tctgttgaat gaagtggatc gggcttctca tcattggcct
     6241 tcacactttt tgtgcgcgtc ctcctgaagt ggattcgcat gttgctgaaa tcgccaggga
     6301 ggcgcagcag ctgaaggctg agattgcaga aacggaagag tgcaagagcg gatgcatttt
     6361 tctgtctgcc tgggatttcg atgggaccat cctgaaaggg gatatggcgg aaggtctgaa
     6421 ggaaggggaa cgcgttgtct acccggggct ccagcagcta gcagtggatc aagggtggtc
     6481 tcgcacttac aggccaggca gctttcagaa gttcctggcc gatcgatccg tgatcgaaga
     6541 aaaggaaggg catctggccg ctctctccat gggcctcaag atatttcgcg gcgtgcccgt
     6601 tatgcgcctt gagagcctcg catcgtctca ttttgaaaaa gttcttagcc gctattattt
     6661 tcgttcttcc atttctattc ttgaaaacac gaagaaggct ggcattgtgc cgtgcatcct
     6721 ttctgcatcg cctcacgttt ttgcgcgttc cgccgcagca acggtcggag ttccgcgcga
     6781 aaacatctac gccatggaga cggagatcgt gcggggattt atgacggata aaattgtcga
     6841 acctgtacct tacgcgaaag gaaaggctca gcggctggag atgattgtag aaaatctaca
     6901 gaagacgacg gggcgtcggg tctttgtggt ggccgcattc ggcaatagct ttcgatctga
     6961 ttcctatttt ctcaagtgga ccctggacca gaagctgccg cgtggccacg ccctggctgt
     7021 gatgatcaat ggcggtccag atgcgggaga gtttcggggg ttgtttcgcg aagtttcgca
     7081 gatcgccact gtatcaggta gataaaattt ccccgcgaca ggtcctccgg cgggcaaact
     7141 aggctttact aaaaacaaaa gggagagttc ggttgcccct atgcgcgtcg agcagatcca
     7201 gccaggcgat gtcagcaccc tcacaggcgc gcatctggtt ctggtattcg ggaacctgga
     7261 tgcgttgaaa ctgcctggct ttcgcgcttc tttgcgtagc aaatacccga acgcgcacat
     7321 cgtaggctgt tccaccgcag gcgaaattgc aggggatcat gtgcgagatg aaacagtgca
     7381 cctgacagcc gttgagtttt ccaatacccg agttcaatct gctttcgttc atcttccgtc
     7441 tcacgggtcc agttattctg cagcgcgctc tctggccatg gcaatcccat cccgggatct
     7501 acgccacgcg attgtctttt cggaagggat ccgaatcaac ggtagcgagc tttcgcgggg
     7561 gctcaaggat ggattaccac ctaacgtgtc cgtaaccggt gggctggctg cagacggaga
     7621 tcgattcgga cagacctggg ttttgtccaa tgacgcagaa ggtcccgatc acgctgtaat
     7681 tgttggtcta tatggccctt ctcttcgcgt tggttttggt tcactcggag gctgggattc
     7741 ctttggcccg gaacgccgca taacgtcttc ctcaggcaat cgactcacgg gcctggacgg
     7801 ggagtctgcc cttgcactgt acaaaaagta tttaggggag catgcatcgt ctctcccttc
     7861 gagcgcactt ctttttcctc ttgcgattcg cgcagaagga gatgatcgtt tcctggttcg
     7921 cactatcctc ggaattgatg aaagcgacca gagcctgatt tttgctggag acatgcctca
     7981 gggatggctg gcgcgattga tgaaggcgaa tttcgatcgc ttgattgatg gtgcggaggg
     8041 cgccgctacg actgcgtcgc gtcgaatagg aaatacatcc cccgagcttg cactgttgat
     8101 tagctgcgtt ggacgcaagc ttgtcctcaa acaaagggta gaggaggaaa tagaagcagt
     8161 gcgcagcgta gtcggcccgg gtgcggctat caccggattt tattcctacg gggaaattgc
     8221 acccgtgtct gacggaagtc cctgcgagct acacaatcaa accatgacca tcacgacgct
     8281 tgcggagggc gaatgagcga ttctttgcac aatttgctcc agaggcagct tcgcaagtat
     8341 ccgttgcctc agatagatgg tgaattgatc gcagccatag atgccgccta tcgtcaattt
     8401 gaccgtgatc gggagatgct ggagcgctcc ctggatctta cttcgcatga actcctcgta
     8461 aagaacgaac agctttctcg ctctgtgacg ttgcttcata ctgcactgga atcgacagcg
     8521 gatggcattc tggtaactga tttgaatggt cgcgtgatcg ctcgaaatgg ccgcctgcaa
     8581 gccatgtgga ctcttcctct ggccacaatc gaagcaggcg aagatgcaat cattcagcat
     8641 tttcagggca tggtcatcca tccaggcagt tcgctcaacg aaccggtgca atccggatcg
     8701 gaagtgcgcg aactatggaa tctaaaggat ggacgcttct ttgattgctg cattcgtcct
     8761 catttgatcg acaatcgtgc tgtgggacgc gtctggaact tccgtgatgt aacagaacaa
     8821 atgaatgcgc gcgatgatct agtgcatgct gcatttcatg atccgctcac cggcctcccg
     8881 aatcgatctc tgtttatgaa ttcgcttgat cgtctcatta ctctcgcatt gccggatgcg
     8941 cgcatgtttg cagttttctt tttggatctg gatcgtttca aagtgatcaa tgatggtctc
     9001 ggtcatattg caggtgatat tctgctcgtt gagattgcgc gaaagctgag ggccatgatt
     9061 ccaccgcact gcatggtggc gcgtctgggt ggagatgagt ttacagtgct tgcggagaat
     9121 gcggcggacg agagagaaat catccacatc gcagaaacca ttctgcgcga aatcaaatcg
     9181 cccatgatgg tggatggaca cgaagtttac accacagtga gcattggaat tgcgctcagt
     9241 acgggggact acagacgccc ggaagaaatg attcgcgacg cggacactgc catgtaccag
     9301 gcaaagaaac agggcaagtc caggtatcag atctttcagg cccagatgcg tgcgcgggcg
     9361 ctcgaagcgg tcagagtgga agcggatctc agacgtgcat tgcccggtcg agaattcgag
     9421 ttattttttc aacccattgt ccgtgcgcaa gaaaggcaag tgattggctt tgaagctttg
     9481 atcaggtgga atcatccaga gagaggatat atttctccat tggattttat tagcgttgcg
     9541 gaggagactg gcctcattgt tcagatgggc gaatgggtgg tgcgcgaagc gtgcaaggcc
     9601 gccaaacgat cgttaggcgg aagatacgtg gccgtgaatc tttccgcagt tcaactgcaa
     9661 cagccacatt ttgtggaaac gattcgttct gctctgatag gcgcggccct tcctcctgag
     9721 ttgctgctcc tggaattgac agaatcggtt ctgatggaaa gtgatccggc tgcactccag
     9781 gcacttggca ttcttcggga catgggagtg tctctggcca tagacgattt tgggactggc
     9841 tattcttctc tcagttactt aaaaaaattc cctgtaaacg ttttaaagat cgatcgcagt
     9901 tttgtccgtg aaactccggc ggacaccagc agctgtgaga tcacccgagt gattctgtcc
     9961 atgtgccgca ttctagggct tcgcgtagtc gcagaggggg tggagaacga tgcgcagctt
    10021 cagtttctgt ccggtgaagg ctgcgagaac attcaaggat tctttttttc cagaccagtt
    10081 cccgaaaaga acatcgactc ggtgattcga tcgatttctg gtgtctgaat gctcaagaag
    10141 attcttccac tccttacact tctctttggt gctctagtgg catccggtgc gggtttttac
    10201 cttgtgcgcg tgcgcgaaac agcgctcgcg gagtacatca agcaggccca tcaaatagag
    10261 cgtgaattcg tgacgcgcac tcctatctat gaggatttcg caactccaga catggagatg
    10321 actcttcgca agtacttgct catggatcat ctacgcgtcg ctgagaaggc aggcgttggc
    10381 gcagttgcca cggacgaaga agtgcaaaag ctggtggaag aaggcaagct ggttccagcg
    10441 cgtcccgatg aagacgctga ttttcatttt tataacgttc caaaagaagg caggtttctg
    10501 actccgcgcg ccagggaagg tctcgaattg attgcacaaa ggtttcagaa aaatattgaa
    10561 aagagatacc agaagcatcc tccagtgcga atcgctgttt cttctgtcct gcgcaccact
    10621 ggctatcaga aagatttgca aaagaaaaat ccaaacgctt cgtttgcatc ttcgcatagc
    10681 tacggcgtca gctttgatct gttctacgac gattactacg tgaagcttcc tcctgtcagt
    10741 cccggaattc cagatgagct acgcactcga ttgggttatc tcctggggga tgctctcatg
    10801 cgtcagtttc gagcgatttt aatggagact ctgattcaac ttcaggagga aggagaaatt
    10861 tatgccatcc tcgaaaagaa ccagcgctgc tatcatgtga cagtcctgga aaagaaatag
    10921 cttgccaggc tgctgaaatg gaaagagagt cgaacgtgga ccggctaacg gaacagctcg
    10981 aagagttgca gcgcggccgc cagctcatcg tctacgctct cgtttttttt gcagtcatgt
    11041 ttgtagcaga agccatcgca ggcttcggat ttggtcgctg gttttcccac agactcgagt
    11101 cagaaaggaa aagcgaattc tcgcgcgcct ttgacaacta tcttcaccag aggtggaatt
    11161 tccactctgg tgctgtgaca ggttacgcct ggtgggctga accatggaac ctcgccagga
    11221 cgggcagatg ggcagaagcg aaagctctct ttgaagggga tccgactctc cagggctcat
    11281 ttgattattt cgcagtttat tctcagtctg atacgccccg gtttgagttc ccaggagccg
    11341 gaaatactcc tctggtgatt ccgccggaca ttgttcgctt cctcgtggaa tcacgcagca
    11401 tgcagcgagg ggaaacgaat ttgattcttc gcatcggaga caagccgtac ctggtcaccg
    11461 ccgcggcgat ggcagacgcg cagggccgtc cacaaatacc cggtatatgc gtgtttgcat
    11521 tcagcctcga tcgatttctc atttcagcac gcgagattct tcctgcaacc ctggagcttt
    11581 cagaagacgg aagcggattc ttgagtgttc cacttgttgc cagcatgcga tcaacggatg
    11641 cccctctata cgttcgcata acgcctaaca cattgatgga ggatttgatt cgcaatatcc
    11701 ttcttgcgtt tcttgcattt cagattgtgc tgagcattct actttttgcc gtgatttctc
    11761 cgcgttacgt tagagaaaga accatgcgcc ttgagaagat ggtgcgtgcg tctcgcatct
    11821 tgaatcgtga gctggaattg cgagtggacg aattgacggc cgcgcgcacg gagattcaga
    11881 aatcggaaaa gaagtatcgg gatcttgtag agagttctcg cgaaattatc ttctctattg
    11941 attcgaaggg caacattact actatcaacc aggcagtgtc ttctttgctt ggatttcagc
    12001 cagaaagttt gattggaaaa agcatccttg acttgattta cgtgagcccc gatcgttcca
    12061 tgaatttcag gcgcgaattt atttctgaac aaattgaatc agtgattcaa aatcagaagg
    12121 tcgcgagctt caagacggag ttcgtaactc gctacaatga gcccagagag cttgagacaa
    12181 ctctggagta tgttcctctt gatgggggat atgcgatttt tggcaaagct gccagcatgg
    12241 tagaagatat gctcgccact tattgcgaaa gagagtctat gaaattcgta atcggtaact
    12301 atctgactct ctcagaacaa atcagtcaac gcgttacgaa taacctgggt cttcattgcg
    12361 acgcgggcac gactttgcag attagaattt gcgtaagtga aatgatcatc aatggaattg
    12421 agcacggaaa tttaggcatt acctacgaag agaaaacaaa tgcgacagaa gatggttcct
    12481 atttggctct gttgcgccgc agacagtttg aagcacctta ttccagcaga aagctcaaag
    12541 tagtgtattc tctcaatccc aggagattgt tgattgtggt tcaagacgag ggagcaggat
    12601 tcaatcacac tcaaatcctc aaagacaact ccatggaagc caataagaaa atgcatctgc
    12661 atggccgtgg aatcacaatg gcaaagagcg tgtttgattc tatgcgttac aatgaaaaag
    12721 gaaatcgtgt agctctgtta aaacggtttc gcaatcaaac tccgccaacg tctcccggat
    12781 ctacaatcgt gtaagttaag gacgcggatg catccaaaag cgcctgcatt tgtgcgcgaa
    12841 tttcattggt tagctgttct acgtattcgc cttttccttt ttgaaatgcg tcgtagtgtt
    12901 ctcccagcgt gaatggcttt gcaaatgtga tgactgctct gcgaggcaat ggatgtggtt
    12961 cgccgcccag tgcacgcggt gtgcgaccaa gcgccaggga ttcgaagcgc tgcaaccatt
    13021 cgtaaaatct ttccggagtg ggtcttgaaa ccagatagtc gcgcttgatt acgatgaagt
    13081 catatgcttt gatgcattcg cgatttgcag ctgccagttc agacggagta agaacaggaa
    13141 gctcaggaga ttcatagcca acttctaaga gttcaatgat acttgtaaga tggc
//
LOCUS       DAOQJL010000032        13184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2305_length_13184_cov_4.819941, whole genome shotgun sequence.
ACCESSION   DAOQJL010000032 DAOQJL010000000
VERSION     DAOQJL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_13184_cov_4.819941"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..993
                     /locus_tag="PLG78_02505"
     CDS             232..993
                     /locus_tag="PLG78_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE22006.1"
                     /translation="MAAAESIQAGLNPAFLLLGCIVLFSYTTQAITGFGSTVIALALS
                     AHIYSIPQLLPVLVGLNLPLCLYFVIQHRKSIESTLVFKEILPWMSAGVGVGLILLAS
                     IPVPALKPMLGILIVIFAVREGIRLMRRTGSMPAMSNAQFRFWTFASGITHGIYASGG
                     PLLVYAVSRKNLGASVFRSTMMTIWLFFNFFLIGFYAYEGRWSSQLVEAIFLLLPLIP
                     IGIYLGEFLHHRISVRTFWFVVQSVLLVSGVSFLV"
     gene            1082..2821
                     /locus_tag="PLG78_02510"
     CDS             1082..2821
                     /locus_tag="PLG78_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE22007.1"
                     /translation="MAELSGGELIARILKQEGVKVVFGIIDGTYFGFYSSLRKNGIQL
                     IGPRHETSALHMAAAYARLTGELGVCMASNGPGVANALPGVAVENVEGNRVLLITSAR
                     RVGIMYPDRGGTYQCFDQTGVIRPMSKWSEHVPNFGRITEMTHTALRMSWTGRPGVVH
                     LDVPEDIINTKVKASPSLLSKNKFRQTEPLHPSEDQVERAAKMLLSAGMPLIHAGSGV
                     IHALAFDELARTAELLDAPVTMSWCARGAAVETTRVFPMIDVAMNNKLRNDADVVLTL
                     GSRLGETDWWGKAPYWRHPSKQKMIQVDIEGRWMGANKPIDLGIQADVKVFLQMLNAK
                     IARARVPSRKTAIDRYNAERNRERAKLAENLKDDSSPMYPGHMAAIARKVFPDDTILV
                     ADGGNTTIWANFFWDVRKPNTILSTFKMGMLGAGVAQALGACVARPKSKVLCIIGDGA
                     MGFHPQEIETAVRNKLKPIYVVACDRQWGMVKMNQQFALKPIKTLIKKSLGPHETINA
                     DLGEIRFDLLAQSMGAHGERVSTPAEFKKALERSLAADKCAVIHVDVNPVKHMWVPGL
                     IYFKDMHKEPKGK"
     gene            2818..3771
                     /locus_tag="PLG78_02515"
     CDS             2818..3771
                     /locus_tag="PLG78_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22008.1"
                     /translation="MKRILVTGAAGYIGSLFIAGLAAKKSGAKILGVDVREPLHPVRG
                     TNFEKCDVSSTELRAVFKKFKPDTVVHLASIVSPPKNSDPQLEYRVDVLGTKNVLEGC
                     AETKTKKIIVTSSGAAYGYHSDNPEWLKETDQLRGNDSFPYSRHKRLVEEMLSEYRKK
                     HPSLTQLIFRPGTVLGAGTRNQITNLFERSFVPGVAGSDSPFVFIWDQDVVECLIRGA
                     TTSKTGIYNLAGDGKVDLPEIARILGKKFIPIPAALLQTVFAICKPLGLVRYGPEQVD
                     FLRYRPVLSNKMLKEKFGFIPRKTSREVFQYYIENSPHLRK"
     gene            3773..5032
                     /locus_tag="PLG78_02520"
     CDS             3773..5032
                     /locus_tag="PLG78_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22009.1"
                     /translation="MENEGKRSRGQAVFTFVLLFLLYFFDYADRTVVNSLFPFLKKDW
                     GLTDAECGSLTSIVTFTIAAGTLPISVLIDRWSRTRVIGTMAVIWSLFTAACGFAGSF
                     AQLFAARIGIGLGEAGYAPGGTAMIAGLFSDKRRSLFMGIWNASIPLGAATGVAVGGY
                     VAQHYGWQRAFGLVALPGLVVSLLFFFVKDYKTVKLSENQKHMGMLPIAKKFLATPSL
                     ILTYIAFAANNVVTSALLVWLPTFLHRTQGIPMDQAGPKGGAVMLLAIVGAPLGGFLA
                     DLWHKKKPNARPVFAGLSALVTTAVFWVAFSLFSHGSSAQYGMILVGGLVAVSYVPAG
                     AALTQDVVHPGLRAVSYSLCVVSMNMLGASVGPLLIGSISDHTDIATALSVLPAFSLV
                     SALFFFAASKYYVRDVNRAQSIENVAT"
     gene            5089..5481
                     /locus_tag="PLG78_02525"
     CDS             5089..5481
                     /locus_tag="PLG78_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22010.1"
                     /translation="MKKLIPILSILLIGASALFAYSFEIKDVGYRKWSGDDCKAFLRK
                     NDGYCTSGDCKALLRANDGYCDTNDCKAVIRGNDGYCQSGDCKAILRKNDGYCETDDC
                     KAFLRKNDGYCQTDNCKAFLRNNDGYCR"
     gene            5554..6441
                     /locus_tag="PLG78_02530"
     CDS             5554..6441
                     /locus_tag="PLG78_02530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE22011.1"
                     /translation="MPKISIVIPALNEEQDLPHLLESIKNQTFTDYEVIVADAGSKDK
                     TIEIAKSYGAHVVPGGMPGPGRNRGAAAAKGEFLFFFDSDVILPHDFLQGALAEMQDR
                     FLDLATCEFKPDSDLRLDQLLFHLANLSVKVNQSFNPRAAGFCIFMTKRLFERVGGFD
                     ETLKLAEDHDIVQRASVFRPLRVLNNVELKVSVRRLKKEGRLSLIGKYMQVELHLLLK
                     GKVTEDIVEYEFGAFNEGDKQSAKKLVDDLEGRIIQIEQQYNQFAVKYLQPKQPDGEK
                     PDSILSALQGVAKAIFEKR"
     gene            complement(6450..7022)
                     /gene="pyrE"
                     /locus_tag="PLG78_02535"
     CDS             complement(6450..7022)
                     /gene="pyrE"
                     /locus_tag="PLG78_02535"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNE22012.1"
                     /translation="MGPLAKELFEATRNHIYQFREEPFTLASGRKSRHYFNCKKITLV
                     PGRLHMLARVLRDEVIPAAGLSPEAVGGLTLGADPIGCALSLAYHEAGKQVVPLIVRK
                     EAKSHGTGQKIEGEWKGVHEVLVLDDVITTAGSTLQAVSAFREAGLVVRHAVCIVNRE
                     EGGEQALREQGITLFQVFKKSDFSLVKDPD"
     gene            7021..8805
                     /gene="lnt"
                     /locus_tag="PLG78_02540"
     CDS             7021..8805
                     /gene="lnt"
                     /locus_tag="PLG78_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNE22013.1"
                     /translation="MASPRSAGNTKKNEGLPLPGFFANPWIEVIAGCIVFGGLCTLSL
                     DPFLIPGIVWICSWPLFHFADRYQNHAWKLLGIGALGAMFLCLFSFYWMLHLFSTYGG
                     LNIALGLLIFIPYTILLNLKIPMFLVAFGFAKRFETKLPPMWLVAATLCVLTDYLAPQ
                     VFPWYWGNLAGKNRPLSQVAEFAGVYGISFLHFAGSYALYALFKTILHSIAQGQEKAS
                     EAVMSALKSGAWSDNRGKWAVPTLFAIVLVWGYVRLYQMANVEKQSPHVRVAMIQPNT
                     RLPRAGEGLIPRDEVRDILENRIPSLVGKAAAAGPLDMIVLPESAVPNNLTTQDNMVT
                     RIEGIYSPEFASMMQKLATKYKATVYANEVAADVKLDRVTGRPRPIEFNSSIVYSPDG
                     GRREYYHKRTLLAFGEYIPGAEFLDSSGLIALVPEAVRYSRFERGESAHNLTYYVKKE
                     GAQPAGTFLPLICYEVLIPEYVRSYFDDSNPGFIVNITQDGWYGKTVETYQHFGLGRI
                     RAIELRRAIVRSTNSGSSGFVNLSGDYVEPLLGPRLSGQEVEEVQVWDVPVNWVDPTF
                     YVRFGNLWILILAVGVAGWTTVRVRSRK"
     gene            8802..9710
                     /locus_tag="PLG78_02545"
     CDS             8802..9710
                     /locus_tag="PLG78_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE22014.1"
                     /translation="MKKTLTIAMVLFVAYATAAFYFSGEITTFKVKSKETVLAEDNLR
                     GYADVDLPRPEDVSVTGGQVELNGWLFVNRKNRRCGVILHHGHAANRNGMLKYAKLFW
                     PRGCHLLMFDARHHGQSAGTFATWGVRERDDLVIVADWFAKRTGLPHEKIGLFGASMG
                     AAIVLQAAPLTPDFAFVAADSPFRDLDSILKYRGSTLYGKAPILALYPAASWIAGLRG
                     NFRTEDASPLSAAPHIEIPVFMTHVKDDDAIPFTHSEEVFAAIGHNRKTLHLVDKGGH
                     CGLIKTNPTLYAAWMDDFLKKFAPEF"
     gene            9716..10336
                     /locus_tag="PLG78_02550"
     CDS             9716..10336
                     /locus_tag="PLG78_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE22015.1"
                     /translation="MSNNVYDRIFARFYDTFMEKPEKVELARRRRRLLRDLKGNILEI
                     GAGTGASFQWYGPAAHVLAVEPSLPMASRARARINGKDIELVEAGIGDPILDERIKPG
                     SLDAIVCALVLCTVPDLSGALSRIESWLKPGGKLVLLEHVRSHGKITGAFQTAINPVW
                     KVFAGGCSLNRPTDLLVKNRKFKVQSEQQFRLTMPFYEATLERLSD"
     gene            complement(10340..12313)
                     /locus_tag="PLG78_02555"
     CDS             complement(10340..12313)
                     /locus_tag="PLG78_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE22016.1"
                     /translation="MLPTAQASERAALKRAIFLDPAVSLGKDLAMEIHPLDELYGSDG
                     MGAGLSLGVAIAQLATFESHRDQLWNWILSHPETELRLIVISPQDHPKSYFRPIPEEI
                     IHLVLPSNVSQQVLTDVIEGAFHVLKLHNDKVQLQSRLALSYQDIRRLTRVGQALATE
                     RNFETLIGLILQLAREIVAADAGSIYVVERPRHNEKPTHIRFKKSAMNLAADEFLLPI
                     DRNSIAGYVALTGQPLMIENVYALSGQEGYSFNYEFDRLHNYYTKSMMVIPMKNHRDE
                     VIGVIQLINKKRSSARKLTLEDMKGREVVPFTDNCRELVSALAGQAAVAIQNTILVQD
                     INNLFEGFVKASVFAIEQRDPTTSGHSFRVAEFTVGLAQAVDRLSSGKFADLKFSPDQ
                     IRELRYASLLHDFGKVGVRENVLVKAKKLYEPDMELIRWRFHYIRKALEKDVLEKKLK
                     YYKENGAVGFAQYEKYVDLEFRETMEEVNEFFRIINESNEPSVLEAGNFARLEEISQR
                     KFRVDDGSDVPFLKQNELVSLSVRKGNLDRQERLEIESHVSHTYKFLVQIPWTSELRR
                     IPDIAHGHHEKLDGSGYPLGLKSQEISPQTRMMTISDIYDALTASDRPYKKSVSQERA
                     IDILKLEVKDGHVDSDLLDIFIEAEIFKVQYRS"
     gene            12383..13069
                     /locus_tag="PLG78_02560"
     CDS             12383..13069
                     /locus_tag="PLG78_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE22017.1"
                     /translation="MSRSFFELEQNIRLILNQSQELTLQSSKVNEKVEKYILHILKMI
                     LTRYDRESLLELVYTITKELAINGAKANQKRLFFDEHGLDITNERDYARGMLEFRQAF
                     SDEMNEVYGIKSREKGVYVKIYLSHQPNGLCIEVENNTAIAPAEELRLREKMKKSMSY
                     NDIAEFYMDNMDNTEGAGLGIALIMIMMKSEGLDPALFRIGTLEDRTIARVEIPFNAE
                     YVSKRSQIGR"
     gene            13086..>13184
                     /locus_tag="PLG78_02565"
     CDS             13086..>13184
                     /locus_tag="PLG78_02565"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNE22018.1"
                     /translation="MLVSLCRAKIHRATVTQTELQYPGSLTLDLDLI"
BASE COUNT         3373 a         3264 c         3341 g         3206 t
ORIGIN      
        1 ctcccacaga tgttcccatt gcatacacgc gagggtaaag cggcttcagg aatgcgagtt
       61 tgagagaaga gaaagcagcg agctctgcgc gcaacccctg agtcactata tcgtaagaat
      121 gggccagtac aattcgcacg gaccctgaat cttacggcgt gcacagagtg gtcaagcaac
      181 tgtggttttc aacagttgcg cgactggcat gcaaatggta gaatgaacca gatggcagcc
      241 gctgaaagca ttcaagccgg gctaaatcca gcattcctgc ttctgggctg catcgttcta
      301 ttttcctaca caacgcaggc gatcactgga tttggaagca cagtcattgc gcttgctctg
      361 agtgctcaca tctactccat ccctcaactt ctgccagtac tcgttggact caatctccca
      421 ctctgccttt atttcgtgat ccaacataga aagtccattg aatccactct ggtctttaaa
      481 gagattttac cctggatgag cgcgggagtg ggcgtcggct tgattctact tgcgtcgata
      541 ccagttccgg cgctcaaacc catgctcggg attctcattg tcattttcgc agtgcgagaa
      601 ggaattcggc tcatgcgccg aactggatcc atgcctgcca tgagcaacgc ccagtttcga
      661 ttctggacat ttgcctctgg aatcacgcat gggatttatg catctggagg tccgcttctt
      721 gtgtatgccg tgagcagaaa gaacctgggc gcttctgtat ttcgaagcac catgatgacc
      781 atctggttgt tcttcaattt ctttctaatt ggtttttacg catacgaagg gagatggtcc
      841 agccaactgg tggaagcgat cttcttgctt ttacctctca ttccgattgg tatttatttg
      901 ggagagtttc ttcatcacag gatttcagtt cgcacgttct ggtttgtagt gcagtcggtg
      961 ctcctggtat ccggggtgtc ttttttagtg taggaatagt tgaaaatgac aagtgaacag
     1021 acacccaaaa ttagttaaga aacatatact acagatttga aacaaaaagc gggggatcaa
     1081 aatggcagaa ctgagcggcg gggaactcat tgcgagaatc ttgaaacagg aaggcgtgaa
     1141 ggttgttttc ggaatcattg acggaaccta ttttggattc tattccagcc ttcgcaaaaa
     1201 cggaatccaa cttattgggc ccaggcatga aacgagcgca cttcatatgg cggcagccta
     1261 cgctcgtctg acgggggaac tgggtgtatg tatggcgtct aacggtcctg gagtggccaa
     1321 tgcgcttccc ggggtagcgg ttgaaaacgt agaaggcaac cgcgtgctac tgattacaag
     1381 cgccaggaga gttggcatca tgtatccgga ccgcggtgga acttaccagt gctttgatca
     1441 gacaggcgtg attcgaccta tgtccaagtg gagcgagcat gttccaaact ttggtagaat
     1501 cactgaaatg acacacaccg ctcttcgcat gtcctggact ggaagaccgg gagtcgttca
     1561 tctggatgtg ccggaagaca tcatcaatac gaaagtaaaa gcaagccctt ctctgctatc
     1621 caaaaacaag tttagacaaa cagaaccatt gcatccatcg gaagaccagg ttgagcgcgc
     1681 agcgaaaatg cttctgtccg cgggtatgcc tctcatccac gcagggagcg gtgtgattca
     1741 tgctctcgca ttcgatgaac ttgcgcgcac agcagaactc ctggatgcgc ctgtgaccat
     1801 gagctggtgc gcgagaggcg cggctgtgga aacaacacgc gtatttccta tgatcgacgt
     1861 tgccatgaac aacaaactca gaaacgacgc agatgtggtt ttgactctgg gttcgcgtct
     1921 cggggaaacg gactggtggg gaaaagctcc ttactggaga cacccttcca aacaaaagat
     1981 gattcaagtg gacatagaag gccgctggat gggcgcaaac aaacctatcg atcttggtat
     2041 tcaggcagat gttaaagtat ttttacaaat gctcaacgcg aagattgcga gggcacgtgt
     2101 accttcgcgc aagactgcaa ttgatcgcta caatgcggaa cgcaatcggg aaagagcaaa
     2161 actggcagaa aacctcaagg acgattcgtc tcccatgtat cccggacaca tggcggccat
     2221 cgcgcgcaaa gtcttcccgg atgatacaat cctcgttgca gatggaggaa acacaaccat
     2281 ctgggccaac tttttctggg acgtgcgaaa acctaatacg attctctcga cgtttaaaat
     2341 ggggatgctc ggagcgggtg ttgcccaggc actgggcgca tgcgtcgcgc gaccaaaatc
     2401 aaaagttctc tgcatcatcg gggatggcgc catgggattt catccgcagg agatagaaac
     2461 agcagtgcga aacaaactaa agccaatcta cgtggtggcc tgcgatcgcc agtggggaat
     2521 ggttaagatg aatcaacaat tcgctctgaa gcctatcaaa actctgatca agaaatcgct
     2581 tggacctcac gaaaccatca acgctgacct tggcgaaatt cgatttgatc ttctggcaca
     2641 gagcatgggt gctcacggag aaagagtttc gaccccggct gagttcaaga aagcactcga
     2701 acgttcgctg gcggcagaca aatgcgcggt gattcacgtg gatgtgaatc cggtcaaaca
     2761 catgtgggtg cccggtctga tctacttcaa agacatgcac aaagaaccaa agggcaaatg
     2821 aaacgcattc tagtaacagg tgccgcaggc tacattggtt cgctcttcat cgcaggcctc
     2881 gcggcaaaaa agagcggagc aaaaatactt ggcgtggacg ttcgggaacc actccacccg
     2941 gtacgcggca cgaactttga aaagtgcgat gtgagttcaa cggagctccg ggcagtattt
     3001 aagaagttca aaccagatac tgtggttcat cttgcatcca ttgtcagtcc gccaaagaac
     3061 agcgatccac aacttgagta ccgagtggat gtgctgggaa ccaaaaatgt actggaggga
     3121 tgcgcagaga caaagacaaa aaagatcatt gttacgtcta gcggggctgc ctacggatat
     3181 cactcggaca atccagaatg gttaaaggaa acagatcaac ttcgcggcaa tgattcgttt
     3241 ccttattcca gacacaaacg actcgttgag gaaatgctct cggagtaccg caaaaagcat
     3301 ccttcgctga ctcaactcat cttccgtcct ggaactgtac ttggtgcagg aacgcgaaac
     3361 cagatcacaa atctatttga aagatctttt gtgcctggag tggccggttc tgactctcct
     3421 ttcgttttca tctgggacca ggatgttgtg gaatgtttga tccggggagc aaccacgagc
     3481 aaaacgggaa tctacaatct agcgggagac ggaaaggtag atctgccaga aatcgcgcgc
     3541 attctaggaa aaaaattcat cccgattcct gccgcattgc tacaaacagt tttcgccatc
     3601 tgcaaaccac ttggcctcgt gcgatacggc cccgaacagg tggacttctt gcgctacaga
     3661 cccgtgctct caaacaaaat gctaaaagag aagttcggtt ttattcccag aaaaacttca
     3721 cgcgaagtct ttcagtacta catagaaaat agtccacacc tgaggaaatg atatggagaa
     3781 cgagggcaaa cgcagtcgcg gtcaggcagt tttcacattt gttcttttgt ttctattgta
     3841 tttctttgac tacgcagata gaaccgtagt gaattctctt ttccctttct tgaaaaaaga
     3901 ctgggggctc acagacgcgg aatgcgggtc actcacttct attgtaactt tcacaatcgc
     3961 cgcaggcaca cttcccattt ccgtgctcat tgatcgctgg agtagaacca gagtgattgg
     4021 cacgatggct gtgatctgga gtctattcac tgcagcatgc ggatttgcgg gaagctttgc
     4081 tcaactcttt gctgcgcgaa ttggaattgg actgggagaa gcaggttacg ccccaggtgg
     4141 cactgccatg atcgcaggtc ttttttcaga caagcgaaga tccctcttta tgggaatctg
     4201 gaatgcatcc attccactgg gagccgccac tggagtcgcc gtaggtggat atgtggcgca
     4261 acattacgga tggcaacggg cgtttggact ggtggctctg ccgggcctcg ttgtttcact
     4321 tttgttcttt ttcgtgaagg attacaagac cgtaaagcta agcgaaaatc aaaaacacat
     4381 gggcatgcta cccattgcga aaaaattcct cgcaactcca agtctcattc tgacctacat
     4441 tgcatttgcg gcaaacaacg ttgtgaccag tgctctgctc gtctggcttc ccacattcct
     4501 gcatcgcact cagggaattc ccatggatca agcggggcca aaaggaggag ctgtgatgct
     4561 ccttgccatc gtgggcgcgc ctctcggtgg atttctggcg gatctctggc acaaaaagaa
     4621 accaaacgcg agacctgtat tcgctggact ttccgctctc gtcaccactg cggttttctg
     4681 ggtcgcgttc agtttgtttt cgcacggtag ctccgctcag tacggtatga tcttagtggg
     4741 aggactggtc gcagtgtcct atgtacctgc gggagcggcg ctgactcagg acgtagtcca
     4801 ccctggcctt cgcgctgttt cttacagcct ttgcgtggtt tccatgaaca tgctgggagc
     4861 atcggtcggg cctttgctca ttggaagcat ttcggatcac acggacattg ccaccgctct
     4921 ttctgtattg cctgcgtttt ctctggtatc ggctctgttc ttctttgcag caagcaagta
     4981 ctacgtacga gatgtgaacc gcgcccaatc catagaaaac gtcgcgacgt aacgtcaccg
     5041 cgtgattttt tcatggacga actggcaaac cggtcgtcaa ataacgttat gaaaaagttg
     5101 attcccattc tatccatctt gctgataggc gcaagtgcgc tcttcgcata ttcttttgaa
     5161 atcaaagatg taggctatcg caagtggtcc ggagatgatt gcaaagcatt ccttagaaag
     5221 aacgacggct actgcacgtc cggagattgc aaggctctac tgcgcgcgaa cgacggttac
     5281 tgcgacacaa acgactgtaa agctgtgatt cgtggaaatg acggttactg ccagtcaggt
     5341 gactgtaagg cgatcttgcg caagaacgat ggctattgcg aaacagacga ttgcaaagca
     5401 ttcctcagaa aaaatgacgg atactgccag acagacaatt gcaaggcctt tctgcgaaac
     5461 aacgacggct actgccgcta aactaccgcc ggccgggtac caaggctttc tttttttcct
     5521 tgtatcccgg cagggcccgg cccctgaatg cctatgccca aaatcagcat cgtcattcca
     5581 gcactgaacg aagaacagga cctgccccac cttctagaaa gtataaaaaa ccagactttc
     5641 actgattacg aagtaattgt cgcggacgcg ggatctaaag acaagaccat tgaaattgcg
     5701 aagagctatg gtgctcatgt tgtgccaggc ggcatgcctg gaccaggtcg aaatcgcgga
     5761 gcggccgccg caaaagggga atttcttttt ttctttgatt cagatgtaat tcttccgcac
     5821 gattttctac agggagcact tgcagaaatg caggatcgtt tcctcgatct agcaacctgc
     5881 gaattcaaac cggattccga tctaagactc gatcaactgc tctttcatct ggccaatctt
     5941 tccgtaaaag taaatcaatc gttcaatcca cgcgcagccg gattctgcat ttttatgacc
     6001 aagaggctct tcgaacgagt gggtggtttt gacgaaactc tcaaactcgc agaagatcac
     6061 gacatagtgc aaagagcttc tgtgtttcgc cctcttagag ttctcaacaa cgtagaactg
     6121 aaagtaagtg ttcgcaggct caagaaagaa ggacgacttt ctctgattgg aaaatacatg
     6181 caggtggagt tgcatcttct gttaaagggc aaagtcacag aagacattgt agagtatgaa
     6241 ttcggtgcct tcaatgaagg ggacaaacaa tccgcaaaaa aactcgtgga cgatctcgaa
     6301 ggtcgcatca tccagataga gcaacagtac aatcagtttg cggtgaagta cctgcagcca
     6361 aagcagccgg atggtgaaaa gccagactcc atcctaagcg cactgcaggg agttgcaaaa
     6421 gcgatcttcg agaagcgctg agcgagactt taatccggat cctttaccag ggagaaatca
     6481 ctctttttga agacctgaaa aagagtgatg ccctgttcgc gcagtgcttg ttctccgcct
     6541 tcctcgcgat ttacaatgca caccgcatga cgcaccacca ggcccgcttc gcgaaacgcg
     6601 ctcaccgcct gaagagtgga gccagctgta gtgatgacat cgtccagcac cagcacttcg
     6661 tgtacgccct tccattcacc ttcgatcttc tgacctgtgc cgtgcgactt ggcctctttg
     6721 cgaacgatca aaggaaccac ctgcttccca gcttcatgat acgctagaga gagagcgcaa
     6781 ccgattgggt ccgcgcccag tgttagacca cccactgctt ccggagacag gcctgccgca
     6841 ggaatgactt cgtctcgtaa aactcgagca agcatgtgga ggcgacccgg gacaagagtg
     6901 atttttttgc agttgaagta atgcctcgac ttccggcctg atgccagagt gaacggctcc
     6961 tctctaaatt gataaatatg attgcgtgtc gcttcaaaca attctttagc aaggggtccc
     7021 atggcatctc ctcgatcggc cggtaatacg aagaaaaacg aaggcctgcc tttgccaggc
     7081 ttttttgcaa atccatggat cgaggtcatc gcaggctgca ttgtcttcgg aggcctctgt
     7141 acgctttcgc tcgatccatt ccttattcca ggcattgttt ggatttgttc atggccgctg
     7201 tttcatttcg ccgatcgata tcagaatcat gcgtggaagc ttctgggaat tggagcactc
     7261 ggcgcgatgt ttctttgcct gttttccttt tactggatgc tccacctctt tagcacgtac
     7321 ggcggactca acattgctct aggactcttg atattcatcc catacacgat ccttctcaat
     7381 ttgaagattc caatgtttct ggtagcattt ggtttcgcca agagattcga aacaaagcta
     7441 cctcccatgt ggctggtcgc ggccactctc tgcgtgctca cggattacct ggcacctcag
     7501 gtattcccct ggtactgggg aaatctggcc ggtaagaaca gacctttgtc ccaggtagcg
     7561 gaattcgcag gagtatacgg gatatctttt ctgcatttcg caggcagcta cgctctctat
     7621 gcactgttca agacgatcct gcattcaatc gcccagggcc aagagaaagc atcggaagca
     7681 gtgatgagtg cactgaagag cggcgcctgg agcgacaacc gcggaaagtg ggccgttccc
     7741 acgttattcg ccattgtgtt ggtgtggggt tatgtgcgtc tctaccagat ggcgaacgtc
     7801 gaaaaacaat ctcctcatgt tcgcgtggcc atgatacaac caaacacacg acttccgcgc
     7861 gccggagaag gtctgattcc gcgcgatgaa gtgcgcgaca tccttgaaaa tagaatcccg
     7921 tcgctcgtgg gaaaagcggc cgcggccgga cctctagaca tgattgtgct gcctgagtca
     7981 gctgttccca acaatttgac gacccaggac aacatggtca cgcgcattga gggcatttac
     8041 agcccggagt tcgcgtccat gatgcaaaaa ctggcgacga aatacaaagc aactgtgtat
     8101 gcaaacgaag tggcagcaga cgtcaaactg gatcgtgtga caggcagacc tcgccctatt
     8161 gaattcaatt cttctatcgt ttattcgcct gacggaggaa gacgagagta ctaccacaaa
     8221 aggactctac ttgcttttgg cgaatacatc ccgggcgcag aattcctgga tagttccggt
     8281 ctcatcgcac ttgtgcccga agcagtgcga tacagccgtt ttgaaagagg tgaaagcgca
     8341 cacaatctta cgtactacgt aaaaaaagaa ggcgcgcaac ccgcagggac gttccttcct
     8401 cttatctgct acgaagtttt gattccggaa tacgtaagaa gctactttga tgattcaaat
     8461 cctggattca ttgtgaacat aacgcaagac ggttggtatg gcaaaactgt ggaaacctac
     8521 cagcattttg gactgggacg cattcgagcg attgaactga gacgtgcgat cgtaagatct
     8581 accaacagcg gcagctcggg attcgtaaat ctgtccggag actatgtaga accattactc
     8641 ggacccagac tctccggcca ggaagtagaa gaagtccagg tatgggacgt tccggtgaac
     8701 tgggtagatc ctacgtttta cgtaagattt gggaatctct ggattctgat tcttgccgtg
     8761 ggtgttgcag gctggacaac cgttcgggtt agatcaagaa aatgaaaaaa acgctgacca
     8821 ttgctatggt gttgtttgtt gcgtacgcca cagctgcgtt ctatttttca ggagagatca
     8881 caactttcaa agtaaaatca aaggaaactg ttctcgcaga ggacaatctt cgcggttacg
     8941 cagacgtgga cctgcccaga ccagaggacg tatccgtaac aggcggtcag gttgagctga
     9001 acggatggtt gtttgtgaac cgtaagaatc gcagatgcgg cgtcatcctc caccacggtc
     9061 atgccgccaa tcgcaacggc atgctcaagt atgcgaagct attctggcca cgcggctgcc
     9121 atttgcttat gttcgatgcg cgccaccatg gccagagtgc cggaacattt gccacctggg
     9181 gagtacgcga acgcgatgat cttgtgattg tggcagactg gtttgcaaag cgtactggac
     9241 ttccgcacga gaagattggc ctctttggtg cttccatggg agcggcgatc gtactgcagg
     9301 ccgcaccact cacacctgat ttcgcatttg tagcagcgga ttctcctttc cgcgacctgg
     9361 actctatttt gaaatacaga ggaagtactc tgtatggaaa ggctcccatt ctggcactct
     9421 atcccgcagc atcctggatc gcagggcttc gcggcaattt tcgcacagag gacgcaagcc
     9481 ccctttctgc ggcgccccac attgaaatcc cggttttcat gacacacgta aaagacgacg
     9541 atgcaattcc tttcacccat tcagaagaag ttttcgccgc catcgggcac aatcgcaaaa
     9601 cgcttcatct tgtggacaag ggcggtcatt gcggtctcat caaaacaaat ccaactctct
     9661 atgcagcgtg gatggatgac ttcctgaaaa aattcgcccc ggagttctag ggtctatgtc
     9721 gaataacgtc tacgatcgaa tttttgcgcg gttctatgat actttcatgg agaagcccga
     9781 aaaagtcgaa ttggcgcggc ggcgacgcag acttctgcgc gatctgaaag ggaacatact
     9841 ggaaatcggc gcggggaccg gtgcaagctt tcagtggtac ggtccggcgg cgcacgttct
     9901 ggcagtggaa ccatctctgc ctatggcaag tcgagcacgg gcaagaatca acgggaaaga
     9961 cattgaactc gtggaagcgg gaatcggaga tccaatcctc gatgaaagaa tcaaaccagg
    10021 aagcctcgat gcgattgtat gcgccctggt tctttgcact gttccagatt tgtctggcgc
    10081 gctttcgcgc attgagtcct ggctaaagcc tggcggaaag ctggttctgc tcgaacacgt
    10141 tcgatcgcat ggcaagatca cgggtgcctt tcaaactgcg atcaatccag tgtggaaggt
    10201 attcgcgggt ggatgttctc tgaacagacc aacggatcta cttgtgaaga atagaaaatt
    10261 caaggtgcaa agcgaacaac agtttcgcct gactatgccg ttctacgaag caacgctgga
    10321 acgcctgtct gactgaagat caggatctgt attggacttt gaaaatctcc gcttcaatga
    10381 atatgtcgag cagatctgaa tccacgtgac cgtccttcac ttccagcttg agaatatcaa
    10441 tggcacgctc ctgcgaaacg gactttttgt aaggcctgtc acttgcagtg agcgcatcgt
    10501 aaatatctga aatggtcatc atcctggtct gaggagaaat ttcctgcgac ttgaggccta
    10561 atggataacc ggaaccatcc agcttctcat gatggccatg cgcaatgtct ggaattctcc
    10621 tgagctctga ggtccacgga atctgcacga gaaacttata ggtgtgactg acatgggact
    10681 caatctccag tctttcctgg cgatccaggt ttccctttct aacagataga ctcaccagtt
    10741 cattttgctt gaggaaggga acgtccgagc catcgtctac tctgaacttg cgctgagaaa
    10801 tttcttccaa ccgcgcaaag ttgcctgctt ccagcacaga aggctcgtta gactcgttga
    10861 tgatgcggaa gaactcattt acctcttcca tggtctcgcg aaattccagg tccacgtatt
    10921 tctcatactg agcaaagccg actgcaccgt tttctttgta gtactttaac ttcttttcca
    10981 gtacgtcttt ctctagagcc ttccggatgt agtgaaatct ccagcgaatc agttccatgt
    11041 ccggctcata cagcttcttg gccttcacaa gaacattctc acgcacgcca actttcccga
    11101 agtcgtgcaa caatgacgca taacgtagtt cgcgaatttg atcgggagaa aatttaagat
    11161 ctgcgaactt acccgaggaa agtctgtcta ctgcctgagc aagtccaacg gtaaactctg
    11221 cgacgcggaa cgagtgaccg gaagtggttg gatctctctg ttcaattgca aatacggaag
    11281 cttttacgaa tccttcaaag aggttgttga tatcttgaac gagaattgta ttctggatgg
    11341 ccacagcagc ttgccctgcc agagcgctga cgagctctct gcaattgtcc gtgaagggaa
    11401 ccacttcgcg ccctttcata tcttcgagcg tgagctttcg cgcggaactt ctcttcttgt
    11461 tgatgagctg aatgaccccg atcacttcat cccgatggtt cttcattgga atcaccatca
    11521 tggactttgt ataatagttg tgaagacgat cgaactcgta attgaagctg taaccttcct
    11581 gaccgctcag cgcgtacaca ttttcgatca tgagaggttg accggtgagc gccacgtaac
    11641 cggcgattga attgcggtca attggcagaa gaaattcatc cgccgcgaga ttcattgcag
    11701 atttcttaaa gcgaatgtga gttggctttt cgttatgtct gggccgctcc acaacgtaga
    11761 tggatcctgc atccgcagca acaatctctc gcgccagttg cagaatcagg ccaatgagag
    11821 tttcaaaatt tctttctgtt gcaagtgcct gacccacacg cgttaggcga cgaatgtcct
    11881 gatagctgag tgccaggcgt gattgtagtt gaactttgtc attgtggagt ttaagaacgt
    11941 gaaacgcgcc ttcgatgacg tcagtgagca cctgctgcga aacgttagat ggcaaaacca
    12001 ggtggataat ttcctccggg atgggacgaa agtaactctt gggatgatct tgcggcgaaa
    12061 tgacgatgag acgtaattct gtttccggat gggacagaat ccagttccac aactggtccc
    12121 tgtgactttc gaacgttgcc agctgtgcga tggccacccc cagagagagg cctgcaccca
    12181 taccatctga gccgtagagc tcatccaggg ggtgaatttc catagcaagg tcttttccca
    12241 gcgatacagc cgggtccagg aaaatcgccc gcttcagagc ggcgcgttcg gatgcctgag
    12301 cggtgggtaa catgattagc tccaggaatc agacttgctc gcaagccaca tagaaggact
    12361 tctggccccg ggcgcccttt ttatgagcag aagctttttt gagcttgagc agaacatacg
    12421 gctaatcctg aaccagagtc aagaactgac tttgcagtcc agcaaagtca atgaaaaagt
    12481 cgaaaaatat attcttcata tcttgaagat gatactcacg cggtacgacc gtgagtcact
    12541 gctcgaatta gtatacacaa tcacaaagga actggccatt aacggtgcga aggctaatca
    12601 gaagagacta ttttttgacg aacacggtct cgatattacc aatgaacggg attatgccag
    12661 gggaatgctg gagtttcgcc aggcattctc tgatgagatg aacgaagtct acggaatcaa
    12721 gtccagagag aaaggcgtgt atgtgaaaat ctacctttcc catcagccaa acgggttatg
    12781 cattgaggtc gaaaacaaca cggcaattgc gcccgcagaa gagctcaggc tgcgcgaaaa
    12841 gatgaaaaaa agcatgagct ataacgacat tgctgagttt tatatggata atatggacaa
    12901 taccgagggc gccggcctcg gaatcgcact catcatgatc atgatgaaga gcgaaggact
    12961 ggatccggca ttgtttcgaa tcggcacgct ggaggaccgc accattgcaa gagttgagat
    13021 tccattcaac gctgaatatg taagcaagcg gtcgcagata ggcagatgaa accagaggaa
    13081 atgatatgtt agtatcactg tgcagagcga agatccaccg ggccaccgtg acccagacgg
    13141 agctacaata tccaggcagc ctgaccctgg acctggatct catt
//
LOCUS       DAOQJL010000033        12993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2343_length_12993_cov_4.139512, whole genome shotgun sequence.
ACCESSION   DAOQJL010000033 DAOQJL010000000
VERSION     DAOQJL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2343_length_12993_cov_4.139512"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1278)
                     /locus_tag="PLG78_02570"
     CDS             complement(190..1278)
                     /locus_tag="PLG78_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE22019.1"
                     /translation="MDAVVIGAGIIGSWVALALARAGLSVAVFDASNKPGDGISGRNS
                     GVLHAGIYYDPGSLKAIHCQRGRNLTEEFLQKHRVPFTICGKLVVGEKDDEPRLITLL
                     ENAKRCGAEGLSIISQPGRNYIGVRGSRALHSSKTGVVDAPAYLSAVRAQAEANGALF
                     LLGRTVTEIDHGRIVTRLMRTAHHEEITCDWIVNAAGLDSDTVAGWAGVSGYEIRPNR
                     GEYFRLRKQLPYPKLVYPLPLLDSTALGVHYTFHLNGDAYAGPNSIWAENKKDYRITA
                     SRREFYLSLSRILEGYEEADLEPGYAGLRPRLFINGIPQRDFVILENPDRVIHMLGIE
                     SPGLTSAPSLAEAIAARVTRGEKIHPNS"
     gene            complement(1281..2210)
                     /locus_tag="PLG78_02575"
     CDS             complement(1281..2210)
                     /locus_tag="PLG78_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22020.1"
                     /translation="MKTTLVAGGAGFIGSHLCETMLAKGHHVLCLDNFQTGSIANVEH
                     LIGNKRFEVIRHDIVDPIRLDVDYIFNMACPASPVHYQANPIRTMKTSVLGTMNLLGI
                     AKRTGARLLQASTSEVYGDPEIHPQPESYRGSVNPIGIRACYDEGKRAAETLCFDYHR
                     FHGVDIRVIRIFNTYGPRMDPRDGRVVSNFIVQALAGTDITMYGDGSQTRSFCYVDDL
                     VGGITNFMECEGQMGPVNLGNDGEFTVKQLAEMVLDMTGSKSKLVYHPLPSDDPVRRR
                     PDLTLARKLISYETRVPLAEGLARTIAYFRSHV"
     gene            complement(2220..3173)
                     /locus_tag="PLG78_02580"
     CDS             complement(2220..3173)
                     /locus_tag="PLG78_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE22021.1"
                     /translation="MKSGVWIALITAVLLTAFWDTRVILPFKLLVVLVHEMWHGISAM
                     AAGATIDRIILHPGEAGETLVRNLKGTWGVIFSVSAGYVGTTTTGALLLRRGLVESFE
                     RLSLCIFAGLILYMTVLFTAPGSLGFNSGLGWSLTLLFAASLGDRYARNGLLIVGTVF
                     IWYSVFDLLDFTRDARQTDAGILARYLKSKLQLSASLQMLSMVTCGLWILVILGIIWA
                     ALGSIIIMPPAPAPAPAPEPVGVPTGASSPLTAAGAPANAAPAPDIAAPIAQAAPTAP
                     GNTSPASKNQVQPIPNHDMAELLALRAEIEKDKNLHAPAPR"
     gene            complement(3187..5157)
                     /locus_tag="PLG78_02585"
     CDS             complement(3187..5157)
                     /locus_tag="PLG78_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22022.1"
                     /translation="MSDIENVPEESFTPILSPAEGRMIPVEPEEDLISDIDLRNLMVQ
                     LSPMHLKDVNAMEMAVENFYVFHDSPEALSHPESLQYLGARDLEWLASKHAIRIFQYI
                     TCEGEQKRLSVHRVVFRPSNLKAEVLIRIKRACIRTSAWAIIRHLQFREHVLSRMETS
                     GEISVWIRKRLLGEFAGSPMRKLSESQVKRLPQIFERLAFEHFLDQDIKPLKELKQGI
                     FYLLTRKPEANGFLEESNPPPADIAEYLRNRMQRPILTQLSDEFHVLTPAIPAPGIAG
                     RSELDYIHDLYCEACRAILIVGMDEDAQRAEYADPMELIHIISAHASFYDEEHWPRGR
                     EFLSELKGIIELLVLSTRMRREMILGGVAQKLISSLNNRFDPLLLDAHDLVSSMPDDA
                     RTLFPDTGAAAAALMSILKSNAELAFFTERRGSGPHEQSFVLFRANLPGAFVQNPERR
                     TFFHAVAKENGYPRGIYDFLISVKSSDPKKLVDDQLELSRAIREWEDHTQKVQKPKSV
                     GWIERLTNWILKLLGFGEFGRRKSDKSKNTTDASGAKAKHAAIPARAQKAIDYVERNN
                     RGLIWLDELMPYLHKTYGSEGEVASIISQDAQDRYVELDSLRPIRRLFVSRHNLELPD
                     WRQKTLDYLENRFSPGPDIRALLEYLKSDRTE"
     gene            complement(5164..6306)
                     /locus_tag="PLG78_02590"
     CDS             complement(5164..6306)
                     /locus_tag="PLG78_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22023.1"
                     /translation="MVGIGWIPIEKENALSLKDMGVRAARMALEDAGVEMIDALYASN
                     MLGSELQGQKHIGAMLADGLGLFGIEALQVDATSASGAAALRAAWLSVASGASDFALA
                     VGVEKMGSTEDATPILARGLNQEEEKGETMVTIVSRLMDLYFKNAMRSGVSEEAMREG
                     FAHFAVNSHQNGMRNPDAFLKKELSLESAMNARVIQGPLRLSDCSPVCDGSAAVLLCA
                     SGMAGSFSDHPVQLSASSCATDRFTVGRRRDPLVLSASFHSVEKAYRQAGITPRDIDF
                     FEVHDAFSIMAVLSLEACGFAERGKGWRAGTEGTIYPGGKLPLSTFGGLKSRGHPVGA
                     TALYQACEIVRQLRGQAGPNQVEGKTALMQSIGGAGTTILTHIFQR"
     gene            complement(6318..7928)
                     /locus_tag="PLG78_02595"
     CDS             complement(6318..7928)
                     /locus_tag="PLG78_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNE22024.1"
                     /translation="MKHSTAMLRIVTTIFILSTAALAAEDLPVRSVLLYPDRAQITRK
                     DNVKLVAGKNRLIIANVSPNLLPDTLRASCAPDCSIVEVSSRLDRFSETVRPRIQALE
                     KKFAAVKEKRDTVSATIQRNQFVLNFVNQLSTAASRVIGEETMRPADSGRWTKAAQFI
                     QNERTARRSLIQKQEEQGRKIDLELAAIQKELDMARAEGEKTARTVEVRLTSSRAQSA
                     EVQVGYNIRGASWSISYGALLAKDGSVEIEMFGNAVQSTGEDWKNVSLGFSTANTERG
                     LERPRVRPIRLRVIEKSTAQSYKLEDRATGNVDATESQPAEAEETGGQTDIETGGTAL
                     IFRVPEPQTVESSNRSKKVPLARFVLKPMETYYRVVPSISESAVLAVKLKNDRVFPLL
                     AGPVDLFRGGRFAGKTTLHYVAGTSEFTLGFAEDRNIVVTRTVNRNRGTAGILGGGKL
                     WTREIRFETENRGNTPADIRIFESIPVSELEEVKISVTESGGFAEEKKGSGIYSKRLT
                     LTPGSKGSAMITYTVRTPEDFPDNLIDR"
     gene            complement(7925..9955)
                     /locus_tag="PLG78_02600"
     CDS             complement(7925..9955)
                     /locus_tag="PLG78_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNE22025.1"
                     /translation="MKATNVLKSAGIVCGMILLTGPQMRAEEDKGELTPDSRITSVML
                     AGGQAIVSRTGRVKLPPGKSTLVFQKLPVTLTDSSLRFGVSTEDRILDIRLDQVTTTE
                     IPDEEARAAEENLRAAQAKLRELTDEYRTLGEMESFARGISADPSEKKWDEKSDRLSP
                     AQWRRSIEFVESTIDSVHAEQRDLRKQMEAAEEDVQVALVVAERLLSRRNLVTKQARV
                     TVQSSGREADFNLSYRTPGADWYPVYSVRVGRAEKEAVKLSAFALVQNRTGEQWDNVK
                     LQLSASNPEDLISLPELREWRIRAAMIAEQNINDATSDKRRQNISPAAPAREMQRQSM
                     DEEAQAPGKPSYAPQTQTNQVVSQLPADNKPVNDELNKTVSNSRNTLKESQGITSGKD
                     ASLLSVLNSRDRAFQDGDYDGAAKYSEEFMGKLGKLRNENQKYFATEEKKSREIREKS
                     LTALQNRTNNLIAPRALRGAAIYETQYSETIPSDGILRKVSLFDSDLQAERVYECAPM
                     HQAFGYLTGHVQYKNDKPLLPGPAQIFYGDDFTGEAVLPETGNNQKIALSLGSTEDIK
                     VTRTENRFRESAGLLGGDLRYKIEVQIKIKNSRREMIVLDVFDRVPRSEDDRIKIEET
                     GLTPAPVERTPEGLARFRLNIGPGAEQIVTLKYTLRHSAEITPSGVEAGPAL"
     gene            complement(9976..10665)
                     /locus_tag="PLG78_02605"
     CDS             complement(9976..10665)
                     /locus_tag="PLG78_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22026.1"
                     /translation="MQNWVTYGVFGLICATTVILSSPTGKGRSAASESGGSDGALAAL
                     FGGSSRAPAPRERSIFESDFWRTGVSDKPIPDEPGRPPRKEGEPDLLEPTSSGNPINP
                     ETNQPYSDAVMEQFSELRKKFPNNSIIPHRKTDQETRAEEQERVTTFALQSKVFQGQA
                     SGEEIDKFYDYQSKGIKDRLELLDYVLQEQGNAMSPEIKSQYEKILTMNKNQMQSIEE
                     ARTRAKSTARN"
     gene            10704..11288
                     /locus_tag="PLG78_02610"
     CDS             10704..11288
                     /locus_tag="PLG78_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22027.1"
                     /translation="MAGPDTQFGRGFLKVGVVLFACTALFLNCGTNTNQPVFPLYVFS
                     TVGSPAISSLTAVQTNLDPLDPQMAFDLRYYITNSEDGFLGYNLYITSSSTSAQGSLV
                     GTATAPYMPNGTSPTFSHVGAVASTAQTSIVTQRVSYQVAPPATKQFQYCELYYFRLT
                     ALTRAGIESAPSAQASSCAAINTALCPSGTPCRP"
     gene            11292..11963
                     /gene="rsmI"
                     /locus_tag="PLG78_02615"
     CDS             11292..11963
                     /gene="rsmI"
                     /locus_tag="PLG78_02615"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNE22028.1"
                     /translation="MPTLSIVSTPIGNLEDVTHRAIRILKESDLILCEDTRKTSILCR
                     HYGIDRPLKSYRIHRLDEDGAYAIDKLREGWSLSLCTDAGTPALSDPGTHLVRLVRAT
                     LPDVSIVPIPGPSALTAALSVCGFRPNPFIFAGFLSIKSGARKRFFEQYRDFDGLIIF
                     FESVHRITKVLAEVREVLPERPVFVGREMTKVYEEYRIVLPEDRITEKGEFTVILGLP
                     GKGEA"
     gene            12031..>12993
                     /locus_tag="PLG78_02620"
     CDS             12031..>12993
                     /locus_tag="PLG78_02620"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HNE22029.1"
                     /translation="MDAGEEIVFFPTEALRDPSGGWNVTIHGNVHNPSTDSTLRKILV
                     DLIRPGDLDSPLEKENYEKRVRLFLVDNLGWKLVRLRIAGRQVEMSVTGSNGHFYGSV
                     HLSEEEVGPFRFRGVIPYESQVADRVFRGESVIISDAGPIIVSDIDDTIKISDARDKK
                     ELLRNTFMRVFRATPGMPDFYKLLQSRGSSFYFVSASPWQLYPELAQFARPIYPPGVF
                     RMKNFRLKDSDFMSLFKDPYAYKIETIEPIVRSFPGRQFVLIGDSGEKDPEAYVEIAR
                     RYPASVKKIWIRMAYGEEPERISKLKATIAPDTLFTFLDPSTLDS"
BASE COUNT         2797 a         3371 c         3377 g         3448 t
ORIGIN      
        1 aggtgaaatc gatcaaagga cagttccctg cgggagcaag ttctctatcg gtggcaggtt
       61 tccagatgca attgataggg agggaaggcg atcttgcaag aattgacgtg ggcccatccg
      121 tgcttacaga cgaaatcacc ctcactgtac agagactgaa atagccggag cggagcgccc
      181 ttaggggcgc tagctgttgg gatggatttt ttcccctcgt gtgactcggg ctgcgattgc
      241 ttcggcgagt gaaggggcag atgtcagtcc ggggctttca atgccaagca tgtggatgac
      301 tctgtctgga ttttcaagaa tcacaaagtc tctctggggg atcccgttaa tgaatagccg
      361 gggtcggagt ccagcatacc caggttcgag atccgcctcc tcgtaccctt ctagaatccg
      421 gcttagtgat aggtagaatt cacgcctact tgctgttatg cgatagtctt ttttgttttc
      481 tgcccatatc gaatttgggc ctgcatacgc gtcgccattg aggtgaaagg tatagtgcac
      541 accgagagca gttgaatcca gaagaggcaa tggatacact agcttcggat acgggagttg
      601 tttcctgagt cgaaaatact cgcctcgatt tggtctgatc tcgtaaccgg atacgccagc
      661 ccaccccgcc acagtgtccg aatcaaggcc tgcggcattt acaatccagt cgcacgtaat
      721 ctcttcatga tgggccgtcc gcatgagacg agttacgatt cggccatggt caatctcggt
      781 tacggtccgc cccaggagaa acaaggctcc gtttgcctct gcttgagcac ggacagcgct
      841 caaataggca ggagcatcga cgactccggt tttagacgaa tgaagagcgc ggcttccgcg
      901 aaccccgatg tagttcctac cgggttgaga gatgatcgaa agtccttccg ccccacagcg
      961 ttttgcgttt tccagaagtg tgatcaggcg aggctcgtcg tctttttctc cgaccacaag
     1021 ttttccgcag atcgtgaacg gaactctgtg tttttgtagg aactcctcgg tgaggtttcg
     1081 tccgcgctgg cagtggattg ctttgaggct gcccgggtca tagtagattc ctgcatgcag
     1141 gacgccggag ttcctaccag atattccgtc tcctggctta tttgaggcat cgaaaacggc
     1201 cacggacaaa cccgcacgag caagcgccag ggcgacccag gaaccgatga tccccgcacc
     1261 aatgacgacg gcgtccaaag ctaaacgtgg cttcggaagt atgctatggt tctggccagt
     1321 ccctcggcca gaggcactct ggtttcatag gagatgagct tccttgccag agttagatcc
     1381 ggccggcgac gaacaggatc gtcagagggg agaggatggt acacgagctt cgatttcgaa
     1441 ccagtcatgt ccagaaccat ctcggccagc tgtttcacgg tgaattctcc gtcatttcca
     1501 aggttcacag ggcccatttg tccctcgcac tccatgaaat ttgtgattcc gccgaccaga
     1561 tcatccacgt agcagaacga ccgggtttgt gagccgtctc catacatggt gatgtccgtt
     1621 ccggccagtg cctgaacaat aaaattcgat acgactctac cgtctcgcgg gtccatccga
     1681 ggtccgtaag tgttaaagat cctgatcacg cgaatgtcga cgccgtgaaa gcggtgatag
     1741 tcaaaacaca aagtttctgc cgcccgcttt ccctcgtcgt aacatgcgcg gattccaatc
     1801 ggattgacac ttcccctgta cgattcgggc tgcggatgaa tttctggatc cccatacacc
     1861 tctgaagtgc ttgcctgcag aagtctggca cctgtcctct tcgcgatccc gaggaggttc
     1921 attgtcccga ggactgacgt tttcatggtg cgaatagggt ttgcctggta gtggacgggg
     1981 ctggcggggc aggccatgtt gaagatatag tccacgtcca gccggattgg atcaacgatg
     2041 tcatggcgaa tcacttcgaa gcgcttgttc cctatcaggt gttcaacatt ggcgatgctg
     2101 ccggtctgga aattatcgag acagagtaca tgatgtccct tggccagcat ggtctcgcag
     2161 agatgcgatc caatgaatcc ggcaccgccg gcaacgagag tggttttcac ggaaccgatt
     2221 tagcgcggag cgggggcgtg taaattctta tctttctcaa tttccgcccg aagtgcgagc
     2281 agctccgcca tatcatggtt cgggatcggt tgtacctggt tcttggacgc aggcgatgtg
     2341 ttgcccggag ccgtcggggc cgcttgagca atcggggcgg cgatatcagg ggcaggggcc
     2401 gcattcgccg gcgctcccgc ggcagtgagc ggggaggaag cccctgtagg aactcctaca
     2461 ggttcgggag caggagccgg ggcgggcgct ggcggcataa tgatgataga accgagcgct
     2521 gcccaaatga tcccgagaat caccagaatc cacaacccgc acgttaccat gctcagcatc
     2581 tgaagggatg cagatagttg tagttttgat ttgagatacc tggccaggat tccggcgtcc
     2641 gtctgtcgcg cgtcgcgagt gaaatcaagc aaatcgaaga cactatacca gatgaaaaca
     2701 gttcctacaa tgaggagtcc gtttcgcgca tagcggtcgc cgaggcttgc ggcaaaaagc
     2761 aaagtcaggg accagcccag accactattg aatccgagac tgccgggagc tgtgaacagg
     2821 acggtcatgt agaggatcag tccagcaaat atgcagagag acaatcgctc aaagctctcc
     2881 accaggccgc gtcgcaatag aagtgcgcca gttgtagtag ttcctacata tccagccgac
     2941 acactgaaga tcacccccca cgtccccttc aggtttctta cgagcgtctc gcctgcttcc
     3001 cccgggtgca gaatgatccg atcaatcgtc gcgccagcag ccattgcaga aataccatgc
     3061 cacatctcat gaaccagtac gaccagcagc ttgaagggca gaatgaccct cgtatcccag
     3121 aacgccgtta gaagcacagc agtgatgagt gcgatccaga ctccggactt caccctacat
     3181 tttcggtcat tctgtgcggt cggatttcag atattcaaga agagcccgaa tatcggggcc
     3241 gggggagaaa cgattttcga ggtaatccag agtcttctgg cgccagtcgg ggagctccag
     3301 gttgtgcctg gaaacgaaaa gccttctgat cggacgaagc gagtccagtt cgacgtatct
     3361 gtcctgtgca tcctgtgata taatactggc cacttcccct tcgctgccat aggttttgtg
     3421 aagataaggc atgagctcgt cgagccagat cagaccgcgg ttgtttcttt ccacgtagtc
     3481 gattgccttt tgcgctcggg ccggaattgc tgcgtgctta gccttcgcac cagaagcatc
     3541 cgttgtattc tttgatttgt ccgattttct tcttccaaat tcaccaaacc ccagaagttt
     3601 caaaatccag tttgtaagtc tttcaatcca tccaacggac ttaggctttt gtactttttg
     3661 agtgtgatct tcccattcgc gaatggcgcg agatagttcc agttgatcat ccacaagctt
     3721 cttcgggtcg cttgatttta cgctgattag aaaatcataa atcccgcgcg ggtagccgtt
     3781 ttctttggcc actgcgtgaa agaatgttct tctttccgga ttttgcacaa atgcacctgg
     3841 tagatttgct ctgaacaaaa caaacgattg ttcatgcggt ccacttccgc gtcgttcggt
     3901 gaagaatgca agttccgcgt tggatttcag aatgctcatc aatgcagctg ccgcagcgcc
     3961 tgtatccgga aacagggtgc gcgcgtcatc gggcatggat gacactagat catgtgcatc
     4021 cagcaggagg ggatcaaagc gattgttcag cgaagatatc agcttttgcg caactcctcc
     4081 gagtatcatt tctcttctca ttcgcgtgga taacacaagc aactctatga tccctttgag
     4141 ctcgcttaga aattcccttc ccctgggcca atgctcttcg tcatagaaag aggcgtgcgc
     4201 cgaaatgata tggatgagtt ccattggatc ggcgtattcc gctctctgcg cgtcttcgtc
     4261 catcccgacg atgagtattg ccctgcaggc ttcgcaatag agatcatgga tataatccag
     4321 ttcagagcgt cctgcgattc ctggcgctgg aatcgcaggt gtgagcacgt gaaactcatc
     4381 ggacagttga gtcagaatag gacgctgcat gcgattgcga aggtattcag caatgtccgc
     4441 gggaggtggg ttactttcct caagaaaccc gtttgcttcc ggctttcgcg tcaagagata
     4501 gaagatgccc tgcttgagtt ccttgagagg tttgatgtct tgatccagaa agtgttcgaa
     4561 agccaatcgt tcaaaaattt ggggcagcct cttcacctgg ctttcgctca attttcgcat
     4621 gggacttccc gcgaattctc caagcaaacg tttgcgaatc catacgctga tctcgccaga
     4681 tgtttccatt ctgctcagaa cgtgttcgcg aaattgcaga tggcggataa tggcccatgc
     4741 agacgtgcga atgcacgctc gtttgatacg aatgagcact tccgctttca gattggaagg
     4801 gcgaaagacc acacgatgaa cactgagtct tttttgttcg ccctcgcatg tgatatattg
     4861 aaagattcga atcgcatgtt tgctcgccag ccattccaga tcgcgggcgc ccagatattg
     4921 cagagactct ggatgcgaaa gagcttcggg ggaatcatgg aagacgtaga agttttcgac
     4981 tgccatctcc atcgcgttga cgtctttgag atgcattggg ctcaactgca ccattagatt
     5041 tctcaaatca atgtccgaga tcagatcttc ttctggctca acaggaatca tcctgccttc
     5101 tgcgggagac agtatgggcg tgaacgattc ttctggcacg ttttctatat cggacatcca
     5161 gtgctacctt tggaatatat gcgtgagtat ggttgtgcct gcacctccaa tactctgcat
     5221 cagtgctgtt tttccttcaa cctggtttgg gcccgcctgg ccgcgcaact gtcgcacgat
     5281 ctcgcaagcc tgatagagag ccgtggcccc caccgggtgg cccctcgatt tcaatccgcc
     5341 gaaagtggag agaggcagtt ttccgccggg ataaatcgtt ccttccgtgc ccgcccgcca
     5401 gcctttgcct ctttctgcaa atccacaagc ttccagagac aatactgcca tgatgctaaa
     5461 cgcgtcatgt acttcaaaaa aatcaatgtc tcgcggagtg atgcctgcct ggcgatatgc
     5521 tttttcgacg gagtgaaaag aagcggaaag aacaagagga tcgcgtctgc ggccgacagt
     5581 gaatcgatcc gtggcacagg acgaagccga tagctggacc gggtgatcgg aaaaggaacc
     5641 cgccattcca gatgcgcaca gaagaaccgc agcagatcca tcgcacacag gggaacaatc
     5701 gctcagacga agaggaccct ggatcactct ggcattcatc gcggattcaa gagacaattc
     5761 tttctttaga aaggcatctg gattgcgcat tccattttga tgcgagttga ctgcgaaatg
     5821 agcgaagcct tcgcgcatgg cttcttcgct cacgcctgag cgcatcgcat tcttaaaata
     5881 tagatccatg agccgtgaga cgatggtgac catcgtctct cctttctctt cttcctggtt
     5941 gaggccgcgc gcgagaatgg gagttgcgtc ttctgtggaa cccatcttct caacgccaac
     6001 ggcgagtgcg aaatcagaag caccggatgc gacagacaac catgccgcgc gcagagctgc
     6061 ggcaccggac gcggacgttg cgtccacttg aagtgcttca attccaaaga gacccagacc
     6121 atctgccagc atggcgccga tgtgcttttg tccctgcaat tccgaaccaa gcatattgga
     6181 agcgtacaga gcatcgatca tttcgacgcc cgcatcttcg agtgccatgc gcgctgcgcg
     6241 tacgcccata tctttgagcg atagagcgtt ctctttttca atcgggatcc agccgatccc
     6301 gaccacactg accggcctca tcgatcgatc agattgtccg ggaaatcctc cggcgtacga
     6361 accgtataag taatcatcgc agaacctttt gatccgggtg taagcgtgag acgctttgag
     6421 taaatcccgg atcccttctt ctcttctgcg aatccgccac tctctgtcac tgaaattttt
     6481 acttcttcga gttcggagac tggtatgctt tcaaagatcc gaatgtccgc gggcgtattg
     6541 cccctgtttt cagtttcaaa acgaatttcg cgcgtccaga gtttgcctcc acccagaatg
     6601 cccgcagtgc cgcggttgcg attcactgtg cgcgtaacaa ctatgttgcg atcttccgca
     6661 aatccgagcg taaactcgct cgttcctgcg acgtagtgaa gtgtggtctt tccggcgaat
     6721 cgcccaccgc gaaagaggtc cacaggtccg gcgaggagcg ggaatacacg atcgtttttg
     6781 agtttcaccg ctagaaccgc gctttctgaa atagacggta ctacccggta gtatgtttcc
     6841 attggtttta gtacgaatcg cgcgagaggc actttctttg agcgattgga tgattccact
     6901 gtttgtggtt cgggtacgcg gaagatgagt gctgtgccgc ccgtttctat gtccgtttgt
     6961 ccgccggtct cttctgcttc tgcaggctgt gattccgttg catctacatt tcctgtggct
     7021 cgatcttcga gtttgtagga ctgagcggtg gatttttcaa tcactctgag tcgaatgggt
     7081 cgcacacgag gacgttctag acctcgctct gtatttgccg ttgaaaaacc aagggagaca
     7141 ttcttccagt cttcgccggt cgattgtact gcgtttccaa acatttctat ttccactgat
     7201 ccatcttttg cgagaagtgc tccgtacgaa atgctccacg atgcgccgcg aatgttgtaa
     7261 cctacttgta cttctgcaga ttgggcgcgg ctggacgtta ggcgaacttc cacagtgcgt
     7321 gcggtctttt caccctctgc tctggccata tcgagttcct tctggatggc tgccagttcc
     7381 agatcgatct tgcgtccctg ttcttcctgt ttctgaatca aagatcgacg tgctgttctt
     7441 tcgttttgga tgaactgagc ggctttcgtc catctcccgc tatccgcagg tcgcatggtt
     7501 tcttcgccga tcacgcggga agccgcggtg gacagttgat tgacgaagtt caaaacaaac
     7561 tgattgcgct ggatggttgc ggaaacagta tcccgttttt cttttacggc tgcgaacttc
     7621 ttttccagtg cctggatgcg tggtcgcact gtctcagaaa atcggtccag tcttgaagat
     7681 acttccacga tgctacaatc cggagcgcag gatgcgcgca aagtgtccgg caagagattg
     7741 gggctgacat tcgcgatgat caatctgttt ttgcccgcga caagtttcac attatccttt
     7801 cgcgtgattt gagcgcgatc cgggtaaaga agcacggaac gcactggcag atcttctgcg
     7861 gcaagagccg ccgtactgag aatgaagatg gttgtgacga ttcgaagcat tgctgtggaa
     7921 tgtttcatag tgcaggcccc gcttccactc cggaaggagt gatttccgcc gaatggcgaa
     7981 gagtgtattt gagtgtaacg atctgttcgg cgccggggcc aatgttcaga cgaaatcggg
     8041 ccaacccttc cggcgttcgt tctacaggag ccggtgtgag tccagtttct tctatcttaa
     8101 tgcggtcatc ttccgatctg ggaacccggt cgaatacgtc cagtacgatc atttcgcgcc
     8161 tggagttctt gatcttgatt tgaacttcga ttttgtagcg cagatctccg cccagaagtc
     8221 ccgcactttc gcgaaatcga ttctctgtgc gagtcacctt gatgtcttct gtgctaccca
     8281 gggacagagc aatcttctgg ttgtttccag tttcagggag taccgcctct cctgtgaagt
     8341 cgtctccgta gaaaatctgt gcggggcccg gtaggagagg tttgtcgttt ttgtactgca
     8401 catgcccggt cagatagcca aacgcctggt gcatgggtgc gcattcatag actctttctg
     8461 cctgcaaatc agaatcgaac agagacactt tgcgtaggat gccgtctgac ggtatcgttt
     8521 cagaatattg agtctcgtat atagcggctc cgcgaagagc gcgcggtgca attagattgt
     8581 tcgtgcgatt ttgaagcgcc gtcaggctct tctcacgaat ctcgcgagat ttcttttcct
     8641 ctgttgcgaa gtatttctgg ttttcgtttc gcagctttcc aagctttccc atgaactctt
     8701 cgctgtattt ggccgcgccg tcgtagtctc cgtcttgaaa tgcgcggtca cgcgaattga
     8761 gcacggaaag cagggatgca tcttttccag aagtgattcc ctgggattct ttgagggtat
     8821 tgcgcgagtt gctgactgtt ttgtttagct catcgtttac aggcttgtta tccgcgggta
     8881 gctggctgac tacctgattt gtctgagttt gaggagcata agatggtttt ccgggtgcct
     8941 gagcttcttc gtccatcgat tgtctttgca tttcgcgtgc aggagcggcc ggcgagatat
     9001 tctgcctgcg tttgtctgac gtggcatcgt ttatgttttg ttctgcaatc atcgctgcgc
     9061 gaatgcgcca ttccctgagc tcaggaagac tgatgaggtc ttccgggtta gaagcggaca
     9121 gctgcagttt tacattgtcc cattgttcgc ccgtgcggtt ttgaaccagc gcgaacgctg
     9181 aaagtttcac tgcttctttt tctgcacgtc ccacacgaac cgaatacaca ggataccagt
     9241 ctgcgcccgg cgttctatag gatagattaa aatccgcttc tctccccgag gattgaactg
     9301 ttacacgcgc ttgcttggtc accaggtttc ttctggaaag cagtctttct gctactacca
     9361 gcgcaacctg tacgtcttct tctgcagctt ccatttgttt tcgcaagtcg cgctgttctg
     9421 cgtgaactga atcaatggtg ctttctacaa attcgatgct ccgtctccac tgagcgggag
     9481 aaagccgatc gcttttttca tcccattttt tttccgatgg gtctgctgag attccacggg
     9541 caaaggattc catctctcca agcgtcctgt attcatctgt gagctcgcgg agctttgcct
     9601 gtgcggcgcg aagattttct tccgcggctc ttgcttcttc gtctggaatt tcagtggttg
     9661 ttacctggtc caggcgaatg tcgaggattc tatcttccgt ggatacaccg aatcgcaatg
     9721 aggagtctgt gagcgtgacg ggtagttttt gaaacacgag agtgcttttt ccaggaggga
     9781 gcttgactct ccctgttctg ctcacgattg cctggccgcc cgcgagcata accgatgtta
     9841 tgcgagaatc cggagtcaat tcacctttat cttcctccgc tcgcatctgc gggcctgtga
     9901 gtaggatcat gccacatacg atgccggcag atttaagaac gttggtagct ttcatattcg
     9961 tctcttttgg atcgactagt ttctcgcagt ggacttcgcg cgcgttcgtg cttcttcgat
    10021 gctctgcatc tgatttttgt tcatagtcaa aatcttttcg tattgcgatt tgatttccgg
    10081 ggacatagcg ttgccctgtt cttgcagaac atagtccagg agttcgagcc tgtctttgat
    10141 tcctttggac tggtaatcgt agaacttgtc tatttcctcc ccgcttgcct ggccttgaaa
    10201 gactttgctc tgcagtgcga aggtggtgac tctttcctgt tcttccgcac gcgtttcctg
    10261 atctgtcttg cgatgaggaa tgatgctatt gttgggaaac ttcttgcgca gttctgaaaa
    10321 ttgttccatg actgcatctg agtaaggttg attggtttct ggattgattg gatttccgct
    10381 gcttgtgggt tcaagtagat ccggttcgcc ttctttgcgt ggcggtctgc caggctcatc
    10441 cgggattggt ttgtccgaaa cgccggttct ccagaaatcc gattcaaaga tggagcgttc
    10501 gcgcggggcc ggggcacgag agcttccgcc aaagagagca gccagagctc catcggagcc
    10561 gccactttcc gaagccgcag atcttccttt gcccgtgggc gacgatagaa tgacagttgt
    10621 ggcgcagatc aggccaaaaa cgccgtaggt tacccagttt tgcataaatg ttgacgtgtc
    10681 ctcttccccg ggtccgatga gcaatggccg ggcctgatac ccaatttgga cgcgggtttt
    10741 tgaaagtggg tgttgtgctt tttgcatgca cagcactttt tttgaactgc gggaccaata
    10801 caaatcagcc agtctttcct ctctatgtgt tttccactgt aggatctcct gccatatcgt
    10861 ctttgacggc agtgcagacg aatctggacc ctctcgaccc gcaaatggcc tttgatttga
    10921 ggtattacat cacaaattca gaagacggct ttctgggtta caatctgtac atcacctcat
    10981 cctctacttc cgctcagggt agccttgtag gaactgcgac tgctccttac atgccaaacg
    11041 gaacatcgcc tactttctct catgtaggag ctgttgccag caccgcgcag acaagcatcg
    11101 taacacagag ggtttcctat caggtggctc ctcctgccac aaagcagttt cagtattgcg
    11161 agctttacta cttccgcctg accgctctta ctcgagccgg gattgaatct gctccttctg
    11221 ctcaggcatc ttcctgtgcg gccatcaaca cagcgctatg tccatcgggc actccctgcc
    11281 ggccctgatt catgcccact ctctctatcg taagcacacc catcggcaat ctggaagacg
    11341 taactcatcg tgccattcgc atactcaaag agtctgatct gattctttgc gaagatacgc
    11401 gaaagacatc gattctgtgc cgccattacg gcattgaccg acctctgaaa tcctatcgca
    11461 ttcacaggct ggatgaagac ggcgcttatg caatcgacaa gctgcgcgaa ggctggtctt
    11521 tgtctctctg cacggacgca ggtactcctg cactctctga tcctggcact catctcgtgc
    11581 gtctcgtgcg cgcaaccctt ccagacgttt ccattgtgcc gattcccggg ccttctgctc
    11641 tgaccgctgc cctttctgtt tgcggctttc gtcctaatcc attcatcttc gccggtttct
    11701 tgagcatcaa gtccggcgcg cgaaagagat tctttgagca gtacagagat ttcgatggtc
    11761 tgatcatatt ctttgaatca gttcacagga tcacaaaagt gttagcagaa gtgcgagagg
    11821 tattgccgga gagacctgtt tttgtaggca gggaaatgac aaaggtctat gaggaatata
    11881 gaattgttct ccctgaagat cgcataacgg aaaaaggaga gtttacagtc atccttggtt
    11941 tgccaggaaa aggggaggct tagtatttca cgcgctctga ttctcagtct tcttgtctgt
    12001 cttcattgca gcacttttct tgtttcgcct gtggacgcgg gcgaagagat cgtttttttt
    12061 ccaacagaag ctttgcgtga tccatctggc ggatggaacg tgacgataca cggcaacgtg
    12121 cacaatccga gtacagattc tactctgcga aagatcctgg tagatctgat tcgtcccggc
    12181 gatctcgata gtcctctgga aaaagaaaac tacgaaaaaa gagtgcgcct ctttctagtg
    12241 gataaccttg gctggaagct tgtgcgcctt cgcatagcag gccgtcaggt ggaaatgagt
    12301 gttacggggt caaacggtca tttttacggt tctgttcatc tttctgagga agaagtaggc
    12361 ccctttcggt tcagaggcgt cattccctac gaatcgcagg tggcagatcg tgtgtttcgc
    12421 ggagaatcag tgattatttc ggatgccggc cccatcatcg tcagcgacat tgatgacacg
    12481 attaagatca gtgatgctcg cgacaaaaag gaattgctga gaaacacgtt catgcgcgtg
    12541 tttcgcgcca ctcctggaat gcctgacttc tataaattgc ttcaatccag ggggtcttct
    12601 ttctattttg tgagcgccag tccatggcag ttgtatccgg aactggcaca atttgcgcgc
    12661 cccatctatc cgcccggggt tttccgcatg aagaactttc gtttgaaaga ttctgatttc
    12721 atgagcttat tcaaggaccc atacgcttat aaaattgaaa ccatcgaacc gattgttcgt
    12781 tccttcccgg gaaggcagtt tgtgttgatc ggggattctg gagaaaaaga tccagaagcg
    12841 tatgtggaaa ttgcgagacg ttatccggca tccgtgaaaa agatctggat ccgaatggct
    12901 tatggggaag agccagagag aatctctaag ctcaaggcga cgattgcacc ggatactctg
    12961 tttacgtttc ttgacccgtc cactcttgat tcc
//
LOCUS       DAOQJL010000034        12910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2377_length_12910_cov_4.505018, whole genome shotgun sequence.
ACCESSION   DAOQJL010000034 DAOQJL010000000
VERSION     DAOQJL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2377_length_12910_cov_4.505018"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLG78_02625"
     CDS             complement(<1..594)
                     /locus_tag="PLG78_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22030.1"
                     /translation="MAYIKKHERGLIFRKGDYVRSLGPGSHLPLWPGHELVILDTHKL
                     FKISKNLNELKADVELMREMKLLDVPDHSVALHFSDGRFQDVFGPGVFAYWNAAVEHT
                     FRIANLSAPVIGPEFDSALLSNPGLREFVQRFQIEAHQKGLLFFDGAYQGVLGPGAHF
                     FWKGPVQLAVTPIDMRQLQIDMTGQEILTMDRVSVRIN"
     gene            1236..2348
                     /locus_tag="PLG78_02630"
     CDS             1236..2348
                     /locus_tag="PLG78_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE22031.1"
                     /translation="MNCKHTIFLPVLFCFIYCQSPMVRGINEDNPALVQSGLDNGTNP
                     NVVIDGEPALHKALVRMAWNAARTLIDHGADLNAPDKMGNTPLMISMGNRNLELTEYM
                     MSRGADAKKKDTLSKTLLHYAAAVDRPDFISMLVKAGVDINAVDLFGYSPLHDACRFG
                     ASRESVSMLLKHGANVSAPNKAGQVPLAYLIVDADRHERSIPGPLALWLLMDRRTRTG
                     VAPEERRKRMVLIAQDILDAGADPNARDNSGSTLLHLSVLNSFPAFSQELLRHGARVD
                     VVDDKGNLPLHLSFYQAIKSSDLSIVSELIERTPDINQANSLGYTPLHFAAILDKPQV
                     IRQLLSRGARRDIKNKDGKLALDLARSDECKELLRL"
     gene            complement(2505..3062)
                     /locus_tag="PLG78_02635"
     CDS             complement(2505..3062)
                     /locus_tag="PLG78_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22032.1"
                     /translation="MQRQLPLQSLFILIFLLGCGESKHLSEARKAALDSKSTDQEALQ
                     SIAAWCSWNPSETRYTGANVDPWQDGYRVTAGYILNPQRRFIKSVDDFKHAVRVEAAA
                     MVICMFRAGKSRKIRRIELSVALPLEQTSQLVNRFEIEEKDIVEIPNFANQRGPLDEK
                     GLEFYVADSKLKATTDNWEGIIVRN"
     gene            complement(3189..4004)
                     /locus_tag="PLG78_02640"
     CDS             complement(3189..4004)
                     /locus_tag="PLG78_02640"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNE22033.1"
                     /translation="MVSANDFVNKLVESVRKKKFADYIKASPEEVAESLRGKHIFVSG
                     EFATEKPGPGQSKVGGNPDLPESLPWLAEEETASPLAFIAQINLGEVHKHDLSGRLPE
                     KGMLWFFTIADGDRAYGEGIDESTSAVLYDPDPGILSPREMPAELLEENEFALIEERR
                     LLFGPSVSLSGYTDSGITDAIVDALREIGGRRGSVHILTQYRPSDYSSDEDEDSGDDS
                     EGSDAEMVSLIEMDGYEISSDIFVEGIIEFVLSEADLVAGKLERASCQFTAGT"
     gene            4135..6879
                     /locus_tag="PLG78_02645"
     CDS             4135..6879
                     /locus_tag="PLG78_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE22034.1"
                     /translation="MIDTSSVGSFLNQLLDEDTPGAEILNDLRVDGFYTGNFGDPAFN
                     WGGFGFLPISGFGDGDIAALRPIFGRPVDQWPVMRVSHVGDPGKTFASSIKTFVAAHI
                     AMYGDVESDDLEDKRDALIEFAEFLGDTTGRARAVLDAMIADEFGGDDSDKYEIAEGE
                     GSMLASYYSLQENDEDSTAWQSFTKSFPEFAPGHISLFEILSDSEQPDLPALLPIAWR
                     VFNLDMQCDGSYKAARKAAKVLSENGGDKIYGQSHLWPAVIRMAAKKNCGKAWLLGAQ
                     ALEKDGNFQEAYNAYKSAAYWIGSEMEENLSINWVGARRCAEQLKDKNVVALFESLEV
                     PEGEDIEKVLTDYDREEEEEAEATAVQKSSEGFFESARTGNVEILKRELDNGAQIDLA
                     EPKSTLTALHFAAQEGHLDAVRLLFERGANINSKNFRSSTPLMLASRAGHLEIVRLLV
                     EKGADLEIVDGRDETALFLAAESGHVEVVKLLVAHGAKLNVRNISKQTPLFQAVFGDH
                     DEVATVLIEAGADVKLKDYSGESALHWAAGREAPELVKLLLKHGANPRAKADDGETPI
                     DRAEAENLDAIVAILKGETPPAETPFSDESMAALARMSKAWDMTLAKVKNVDELNWGY
                     SEQSDEDLATLHVLPKLRCIILMSCSAITDKGMKSLSRCTGLEEVIISNTPVTDVGIA
                     ELAGLPSLKKLWIDGTAVTDACTGHIAVMEKLEWLRLNELSQITDSAFAKLKSLKKLQ
                     RLDLSGSGISDEACEIIGGFNLESLDISKTQITDSGFEKIKNLKHLTSINFSDTKIGD
                     ACFEVLSGFQKLTEIDLDGTMVHGSGIEKLGKLKKLLSLKLGKCPFDVKNSASLKALK
                     TLRVLDLEGSKISDEAVPHLKTMKHLNALIIAKTKISPAGKKILKSALGAQTTFSWNE
                     "
     gene            6845..7996
                     /locus_tag="PLG78_02650"
     CDS             6845..7996
                     /locus_tag="PLG78_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE22035.1"
                     /translation="MARKLLSAGTNEAMHLVGLAAFIAFALTFIPAVRARFIAKLFGG
                     IARQHRVHHWLGFTTGVIILIHLLYETLREPESAFSLEDPFLLLGWIASVLMFAGLFF
                     SVAKTLTHRFWIWFHWSLFISLVAAACHGFAYLHDATLDRLVFYTSSAIVATSTILIL
                     ASRQAFSAWTVTGINEITPNLHEILLKPEKKESRGFKAGSVVFAQFGAGFSRTWHPFS
                     VASCQFSPVMRLLIKSVGTDTSHLEDLREGSSMSIHGPFPEFNVPRKQDQVWIAAGVG
                     IAPFLGLTRCLDFQDSGKIRLIHYQAPDDWMLAGEFEGFQKRYPQFQWKNVMTARATF
                     DELNTLAEGLDNPAFLICGPPAFMKGARKFLKKQGVSSSNIHTEEFSPW"
     gene            7990..8304
                     /locus_tag="PLG78_02655"
     CDS             7990..8304
                     /locus_tag="PLG78_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNE22036.1"
                     /translation="MVTLQNRLLVFVLALSPLVAQERIITSTELKANANGSSCWIQID
                     NYVYDITPYLSEHATTHEYALVKWCGKDATSGWKDKDGKNKSHSRKAATLLRKYRIGA
                     MR"
     gene            complement(8394..8912)
                     /locus_tag="PLG78_02660"
     CDS             complement(8394..8912)
                     /locus_tag="PLG78_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE22037.1"
                     /translation="MKTDNKEMKMELRGEKEVVLTRYFAAPRELVFDCHTKPELMRRW
                     LIGPPGMTLEVCENDLRVGGKYLYVYSDAKGNKAGVYGTFRKVNTPEVVANSENYTMD
                     LSSFDPNLPEDPNATVESRTFTTQGSGTLMTHVCTYASAEVRKATLESGAGPDGMAEC
                     CLVLDKLLLDLS"
     gene            complement(8909..9253)
                     /locus_tag="PLG78_02665"
     CDS             complement(8909..9253)
                     /locus_tag="PLG78_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE22038.1"
                     /translation="MTSLDATFAALADPTRRAILMRLARGDATVMSLAKPFKMSQPAI
                     SQHLKVLAEAGLVSTTVRGQERPRRLETAPLARAIDWIEKYRQMWEKRYQVLDGLLEE
                     LQAMKPKGDQKK"
     gene            9417..11108
                     /locus_tag="PLG78_02670"
     CDS             9417..11108
                     /locus_tag="PLG78_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE22039.1"
                     /translation="MEHVDLYKGIVRNLPNGSIWVFDRNLRYVAAGGEILKQLGIDAD
                     QVVGKTLWEVWGPEQTKVSEPICRAALEGKSTSVEEPFLGRIYWVITHPILDEDGIIV
                     GGMVLTQDITEHRQAALLGTDQKRDLLLRERALNAIDTGIVIVDALAPDLPITYVNEG
                     FTRLTGYDAEEAIGSNCRFLQAEDRNQPAREEIRKAIAEERRTRVIYRNYRKDGSMFW
                     NELTLYPVRENNRVTHFIGIQHDVTDRIEAEETRKSLEENLFQSRKMEALGLLSARIA
                     HEFNNLLGGVMNYAQLLIDRPEDKVQTYAQKILSAGVRGRDVTAQLLSFARIESKNLA
                     PCAIDHLLEEVIDVVKASLPAKIQLVTELGAPEKIVLADRTMLHGALFNLCANAIDAM
                     KDTGLLTIQSYAVVGSSIVRIRVQDTGDGIDPVLLPRIFDPFFTTKGNGKGTGLGLAY
                     TRATVESHGGKIFVDSTPAQGSSFTIELPVSDHKPVEKNIASPGTTGQRERILIIEDD
                     ENILKSATGAAPPCFRTCEVTRKKRRSWLFPAIWIPIPALLSNPTRDLNFYRNPT"
     gene            11214..11510
                     /locus_tag="PLG78_02675"
     CDS             11214..11510
                     /locus_tag="PLG78_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNE22040.1"
                     /translation="MSKRIQIDSELQTFLQAVSGESRQKILMSFLNGKERPMSDLVKS
                     SELGQSTISIQLAILCRAGLLLRRKHGKEVYYRPDRARIISMVDKLSHFLKGCC"
     gene            11587..12450
                     /locus_tag="PLG78_02680"
     CDS             11587..12450
                     /locus_tag="PLG78_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE22041.1"
                     /translation="MKTDKSLKRSIECTIKDFGFPFLLMTYTMTLWFFPQSATFLLLA
                     FAVAGMALEKWIPYHAEWQGIKTDSKIDAIFSGLTILVSVILKSGTTVVLSRAGMESH
                     VWPSWPLPISVLVALVISGLGPYLLHRAAHEWSAIMWQVHAVHHAPDRVYSLNAMRTH
                     PLNAAWNVLAGLGPLLLLGADSQTILIAGSLNNFFSIFNHMNIDFRNPMLSIFFNTSE
                     LHRWHHSTKQELGDKNYSSGAIALWDHVFGTWRLPAERFKSNGAGLFAANDFPGYSLS
                     KQLLRPICRCA"
     gene            12542..>12910
                     /locus_tag="PLG78_02685"
     CDS             12542..>12910
                     /locus_tag="PLG78_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE22042.1"
                     /translation="MCKTIKQKVKFKTDPETLYELLSNSKMHSRITGQKAVISEKIGG
                     SFETLSGKVTGINVDLLRGKRIVQAWRRSDFPAGIFSMAAVTFEKTTDGGTQLVLTHR
                     GVPKDLIAGIEDSWRNHYWKR"
BASE COUNT         3372 a         3343 c         3169 g         3026 t
ORIGIN      
        1 gttgatacgc acgctcacgc gatccatagt caagatttcc tgaccggtca tgtcgatctg
       61 aagctggcgc atatcgatcg gtgtgacggc caactgcacg ggccccttcc agaagaaatg
      121 cgcacccgga ccaaggacgc cctgataagc tccatcaaaa aacaacaatc ctttctgatg
      181 ggcttcaatc tggaaccgct gcacaaactc ccgcaaacca ggatttgaca gaagcgccga
      241 atcaaactcc ggcccaatga ccggcgcgga gagatttgcg atccgaaaag tgtgttcgac
      301 ggccgcgttc cagtatgcga aaaccccggg accgaaaaca tcctgaaaac gcccgtcgga
      361 aaaatgcagc gcgactgaat gatccgggac atcgagcagt ttcatttcgc gcatcagttc
      421 cacgtctgcc ttcagctcgt tgagattctt agaaatctta aatagtttat gtgtatctag
      481 aatgacaagc tcatgacccg gccagagagg taggtgcgat ccaggaccaa ggctgcgaac
      541 gtagtcacct ttgcgaaaaa taagtccgcg ttcatgcttc tttatgtaag ccatacaacc
      601 tcaaaaaggc agagaagaac ccggccgcac gaattctgca cgggggctcg cttgagccgc
      661 tcgccgatgc gaggaacctg cgcggtgttg cagcccgctg gctgctcgag cgcggaggaa
      721 acgcactggc gctttcggca tcgacggccc gcagggcgca tgctccgtcg ctttcgccgc
      781 agccggactc ttctccgttg tgcgatttgg ggctcgccac accctttgaa cagcctgttg
      841 ctctgccgct gagctaaacg gtttcccgtt gaaggactcg aaccttcgac acacaggtcc
      901 catagtgaac gaagcctcga aagaaggcgg catacgcgca gccagagact ggcgcaccgc
      961 gggacttgaa catccctgca cagacttcga tcgaataacg tcgcccggtc ggagtgaaag
     1021 gtcaggcgcc gctgtgtcaa gcggacgtcc ggattttaaa gcggaactat tcgcgtttac
     1081 gaatcgagaa aacggccatt ggcgcctctt cttttcgtct ttggcctcat tccgcaagaa
     1141 ccacgagttg ggatagtata ggtatactaa gtatttcccc ggaatagcct gggtttgcga
     1201 ttgatggatt ttacatgcct gtttgaatcg ggcctatgaa ctgcaagcac acaatctttc
     1261 tccctgtcct attttgtttc atttattgtc agtctcccat ggtgcgggga atcaatgagg
     1321 acaatcccgc tctggttcaa tcgggcctgg acaatggaac gaatccgaac gttgttatag
     1381 acggagagcc tgcactgcac aaagcacttg tcagaatggc atggaatgct gcgcgcaccc
     1441 tcattgatca tggtgccgat ctcaacgcac ccgacaagat gggcaataca ccgctcatga
     1501 tttcaatggg caatcggaac ctggagctca ccgagtacat gatgtcccgg ggagcggacg
     1561 ctaagaagaa agacacactg agcaaaacac tcctgcatta tgcggcggca gtggatcgtc
     1621 ccgatttcat ttccatgctg gtaaaagcag gtgtggatat caatgccgtg gaccttttcg
     1681 gctactcacc gcttcatgat gcgtgccgat ttggcgcctc gagggaatct gtgtcgatgc
     1741 ttctgaagca tggagcaaat gtctccgctc caaacaaggc cggccaggtt cccctggctt
     1801 acttgattgt agacgccgat cgtcacgaga ggagtattcc agggccgctc gctttgtggt
     1861 tacttatgga tcgccgaacg cgaactggcg tcgcccccga agaaaggcgc aaaagaatgg
     1921 tcctgattgc gcaggatatc ctggatgcag gagcggaccc aaatgctcgc gataactccg
     1981 gatctaccct cttacatcta tcggttctga attctttccc cgcattttct caggaactac
     2041 tgcgccacgg agcccgggtt gatgtcgtcg acgacaaggg aaacctaccg ctgcatcttt
     2101 ctttttatca ggcgatcaag agttcagatt tgtctatcgt ttctgaactt attgaaagaa
     2161 caccagatat aaaccaggca aactcactcg gttacacacc cttgcacttt gcggcaatac
     2221 tcgataagcc ccaggtcatt cgccaattgc tctcgcgagg agccaggcga gacatcaaga
     2281 ataaggacgg taaactggcc cttgatctag caaggagcga cgaatgcaaa gaattgctgc
     2341 gactgtgata ccatcgcgtg ttggatgtcc agctcgcaag actctccaag aatcataata
     2401 tttttcaagt atccccccgc gcacgggggg atcgcgctga tagagagtcg cattttgaag
     2461 gtgataatgc gaccaggggg acaagcccgt ccagagttct gagtctagtt gcgcacgatg
     2521 attccttccc agttatcggt tgtcgccttt agcttactgt ccgcaacgta aaactccaaa
     2581 cccttctcat cgagggggcc gcgctgattt gcaaaattcg gaatttccac aatgtctttc
     2641 tcttcgattt caaaacggtt cacaagctga ctcgtttgtt ccagcggaag agccacggac
     2701 aattcaatgc gccttatttt tctgcttttc cctgcccgaa acatgcagat caccatagct
     2761 gcagcctcaa cccgcacggc atgcttgaaa tcatccacgc tctttataaa gcggcgctga
     2821 ggattcaata tataaccggc cgttacccga tatccgtcct gccatggatc aacgttggcc
     2881 ccggtgtaac gcgtttcgct tggattccag gagcaccagg cggcaatgct ctgcaatgcc
     2941 tcctgatccg tcgattttga atccagagct gccttccggg cttccgagag atgcttgctt
     3001 tcaccgcacc cgagcagaaa tatcagaata aagagtgatt gcaatggcaa ttgacgctgc
     3061 ataaaaagac ctcaacatta gtatcaatcc gcacaggagt tatcagatta actcccgcgc
     3121 caaacgcttt tttcccgctc atagtagttg ataaagttta caaatagaaa atccgcagcg
     3181 aaaggcgcct aagttccggc agtgaattgg caagatgcgc gttcaagttt tcccgccacc
     3241 aggtcagctt cgcttaagac gaattcaatg attccttcta caaaaatgtc acttgaaatt
     3301 tcgtacccat ccatctcgat cagggagacc atttccgcat cggagccttc agaatcgtcc
     3361 ccgctatctt cgtcctcatc cgaactgtag tcagatggcc gatattgagt cagaatgtga
     3421 accgaaccgc gtcgcccgcc gatttccctc aaagcgtcga caatggcatc ggtgatgccg
     3481 ctatcggtgt aacctgacag cgatacggaa ggcccgaaca aaagtcgtcg ttcttcaatc
     3541 aaagcaaatt cattttcctc aagtaactcc gctggcatct cgcgcggaga aagaatgcca
     3601 ggatccggat catacaaaac agcagacgtt gattcgtcga ttccctcacc gtatgctctg
     3661 tcaccatccg cgatggtaaa aaaccacagc atcccttttt ctggcaaccg cccgctaaga
     3721 tcgtgcttgt gcacttcgcc cagattgatt tgagcaatga aggccagagg gctcgctgtc
     3781 tcctcttccg ccagccacgg tagcgattct ggcaggtccg gattgccccc aacctttgac
     3841 tggcccggcc caggcttctc tgtggcgaac tcaccagaga caaaaatatg tttccccctc
     3901 agactctccg ccacttcttc aggcgacgcc tttatgtaat cggcgaactt cttctttctt
     3961 acagactcaa ccagcttgtt gacaaaatca tttgcgctga ccataaaccc tcacgactga
     4021 cacaacgaat gtgaatgata tttattgcgt acaaaatgcc ccgacacacg gttccttgat
     4081 ttctgattgt ggtccgaatg cggcgggcaa aaatctgaga aacgagacga aatcatgata
     4141 gatacttcca gcgtaggaag ctttttaaat caactactgg acgaagacac acccggagca
     4201 gaaatactga atgatctccg ggtggacggt ttctacaccg gaaatttcgg agatcccgca
     4261 ttcaactggg gcggatttgg ctttcttccc atttctggtt tcggggatgg agacattgca
     4321 gcgcttcgcc cgatcttcgg tcgtcccgta gatcagtggc ccgtgatgcg tgtttcgcac
     4381 gtaggtgatc ctggaaaaac cttcgcctcc tctatcaaga cgtttgttgc agcccacatt
     4441 gccatgtacg gcgacgttga atcagatgat ctcgaggaca aacgcgatgc tctcattgaa
     4501 ttcgcggaat ttctggggga caccacagga agggcgcgag ccgttctcga tgcgatgatc
     4561 gcagatgagt tcggaggtga tgactcagac aaatacgaaa tcgcagaggg tgaaggaagc
     4621 atgctcgctt cgtactattc gctccaggaa aatgacgaag actcaaccgc gtggcagagc
     4681 ttcacgaaaa gcttccccga attcgcgccc ggtcatatct ctctttttga aatcctgagt
     4741 gattctgagc aaccggactt accggccctt cttccgatcg cctggcgagt tttcaatctg
     4801 gacatgcaat gcgacggatc ctacaaagcc gcccgaaagg cagcaaaggt tttaagcgaa
     4861 aacggtggag acaagatcta cggacaatcg catctgtggc cggctgttat tcgaatggcg
     4921 gcaaagaaga attgcgggaa ggcatggctt cttggtgcgc aggctctgga aaaagacgga
     4981 aactttcaag aagcatacaa cgcctacaag agcgcagcat attggattgg ttccgaaatg
     5041 gaagagaacc tttccatcaa ctgggttggc gcgcggcgct gcgctgaaca gctcaaagat
     5101 aagaatgtag tcgcactgtt tgagtctctc gaggtgcccg agggagaaga tatagagaaa
     5161 gttctcactg attatgatcg agaggaagaa gaagaggcgg aggctaccgc agttcaaaaa
     5221 tccagcgaag ggttttttga aagtgcccgc acaggtaacg tagaaatcct caaacgcgaa
     5281 ctggacaatg gtgcccagat tgatctcgca gaaccaaaaa gtactcttac agccctgcac
     5341 ttcgccgcac aggaaggtca cctggatgct gtccgcctgt tattcgaaag aggcgccaac
     5401 atcaattcga aaaacttcag aagctccacg cctctgatgc tcgcctcgcg cgcaggccat
     5461 cttgaaatag tgcggctgct tgtagagaaa ggggcagatc tagaaatcgt agacggacgc
     5521 gatgagaccg cgctcttcct tgcggccgaa tcgggtcatg tcgaagtcgt gaaactcctc
     5581 gtggctcacg gtgcaaaact caacgtgcga aacatctcaa aacaaacccc tcttttccag
     5641 gctgtcttcg gagaccatga tgaagtcgcc acggtcctca tcgaagcagg tgccgacgtg
     5701 aagttgaagg attattccgg agaaagcgca ctgcactggg ctgcaggtcg cgaagcacct
     5761 gaactcgtaa agctactgtt gaagcacggc gcaaatcccc gggcaaaggc ggatgacggc
     5821 gaaactccca tcgatcgggc agaggcggaa aacctggatg ccatagttgc cattctaaaa
     5881 ggagaaacac cgcccgctga aacgccattc tcagacgaat ccatggcagc gctcgccagg
     5941 atgagcaaag cctgggacat gacccttgcg aaagtaaaga atgtagatga actgaactgg
     6001 ggttacagcg aacaatccga cgaagatctg gcaacactcc atgttctgcc aaagctacga
     6061 tgcatcatcc tgatgtcgtg tagcgcaatc acagacaagg gaatgaaatc tctctccaga
     6121 tgcacgggcc tggaagaggt gatcatttca aatactccgg tgacggacgt cggaatcgca
     6181 gagcttgcag ggctgccatc tctgaaaaag ctctggatag acggtactgc cgtcacggat
     6241 gcgtgcaccg gtcacatcgc cgtgatggaa aaactcgaat ggctccgctt gaatgagttg
     6301 tctcagatca cggattcagc atttgcaaaa ctaaaaagtt taaaaaaatt acaaagattg
     6361 gatctttcag gctctggaat ttcggacgaa gcatgcgaaa tcattggtgg atttaatctg
     6421 gaaagtctcg acatttcaaa aacacaaatc acagacagcg gattcgagaa gataaagaac
     6481 ttgaaacact taacaagtat caacttttca gatacaaaaa tcggtgatgc atgtttcgag
     6541 gtcttatccg gttttcaaaa actaactgag atcgacctgg atggaaccat ggttcacgga
     6601 agcggcatcg aaaaactggg aaaactgaag aaactactgt ccctgaaact tggcaagtgc
     6661 ccatttgatg tgaagaatag cgcttctctg aaagcactga agactctacg agtccttgat
     6721 ctggaaggat ctaagatttc agatgaagcc gttcctcatt tgaaaacaat gaaacatctc
     6781 aacgcactga tcatcgcgaa gactaaaatt tcacctgcag gaaagaaaat cctgaaatcg
     6841 gcacttggcg cgcaaactac tttcagctgg aacgaatgaa gcaatgcacc ttgtgggact
     6901 cgcagcgttc attgccttcg ccttaacttt tattcctgca gtgcgcgctc ggtttattgc
     6961 gaaactattc ggaggaattg cgcgccaaca ccgggttcac cactggcttg gattcaccac
     7021 cggagtgatc atcctcatcc accttctata tgaaacactc cgtgaaccgg aatccgcatt
     7081 ttctcttgaa gatccctttc ttcttctggg ttggatcgca tctgttttga tgttcgccgg
     7141 cctcttcttc tccgttgcga aaactcttac acaccgattc tggatctggt ttcattggtc
     7201 cttatttatc tcccttgtcg ccgcggcgtg ccacggtttc gcttacctcc atgacgcaac
     7261 actggacaga ctagtatttt acacaagttc ggcgattgtg gccacgagca cgattctcat
     7321 tctagcatcc cgccaggcct tttccgcgtg gaccgtgacc ggcataaacg aaatcacgcc
     7381 caacctgcac gaaattctgc tcaaaccaga aaagaaggaa tcgagagggt tcaaggcagg
     7441 ttccgttgta tttgcgcagt tcggtgcggg cttttccaga acatggcatc ctttctccgt
     7501 cgcgtcatgt caattcagcc ccgttatgcg gctattgatc aagagcgtgg gtacggatac
     7561 ctctcacctt gaggatttgc gcgaaggaag ttccatgagc atccacggac cgtttcctga
     7621 attcaatgtt ccgcgtaaac aggatcaagt ctggattgcg gcgggcgttg gaatcgcccc
     7681 ttttctcggg ttaacgcgtt gtctggattt tcaagattct ggcaagataa gactcatcca
     7741 ctatcaggca ccggacgatt ggatgttggc tggagagttc gaaggctttc aaaagcgata
     7801 ccctcagttc caatggaaga acgtgatgac agcgagagca acttttgatg aactaaacac
     7861 tcttgcagag gggcttgaca atccagcatt tcttatctgc ggtccacctg cttttatgaa
     7921 aggagcaaga aaattcttga agaaacaagg agtgtcatcg tccaacatac atacggagga
     7981 atttagccca tggtaaccct gcagaatcgg ctcctggttt tcgtcctggc cctgtctcct
     8041 ctggtggcgc aggaaagaat cattacgtca accgaattga aggcaaatgc aaacgggtca
     8101 agctgctgga ttcagatcga taactacgtt tacgacatca cgccttatct ttcggaacac
     8161 gcaaccactc acgaatacgc actggtaaaa tggtgcggca aagatgctac aagcggatgg
     8221 aaagacaaag acgggaagaa caaatcgcat tcccgcaagg cggccaccct ccttcgcaag
     8281 taccgcattg gggcaatgag atgagcactg tgtcacaata aggggtcgct agacgccacc
     8341 taacaacatg aagatggctt ttctgccacg acctgggacc gtgcttgcga ttctcaggaa
     8401 agatcgagaa gcaatttgtc cagcaccaga cagcactctg ccataccgtc gggacccgcg
     8461 cctgattcga gtgtggcctt gcgtacttcc gcagaagcat atgtgcacac gtgggtcatc
     8521 agagttccgc tgccctgagt tgtgaaggtg cgcgactcta ctgttgcatt cggatcttct
     8581 ggaagattgg gatcaaacga agacagatcc atagtgtaat tctcagagtt ggccacaact
     8641 tccggtgtat ttactttacg aaacgttcca tagacgccgg ctttgtttcc ttttgcgtcg
     8701 gagtagacgt agaggtattt gcctcctacc ctgagatcat tttcgcacac ttcaagggtc
     8761 atgcccggag gtccaatcaa ccatcttcgc atgagctctg gtttcgtatg gcagtcgaac
     8821 acgagttcgc gcggcgcagc aaagtaacgc gtgagcacga cttccttttc gcctctcagt
     8881 tccattttca tttctttgtt gtctgttttc atttcttctg atctcctttt ggtttcattg
     8941 cctgtaattc ctcgagtagt ccgtcgagta cctggtagcg tttctcccac atctggcggt
     9001 acttttcaat ccagtcgatc gcacgggcca gcggcgcagt ttccagcctg cgaggtcgct
     9061 cctggcctcg aaccgtggtc gaaacgagcc ctgcctcggc cagaaccttc aggtgctggg
     9121 atatggcagg ctgactcatc ttgaaaggtt ttgcgagact catcacagtg gcgtcgcccc
     9181 tggcgagcct catcaaaatg gcacggcgcg ttggatctgc cagtgccgcg aaggttgcat
     9241 ccagagaagt catatatgca ttcacttata taagcatata attatatatc tggcgaacgt
     9301 caagcgattt atgaaatttt ctcgggagtc tctgccgcgt tgcgaatgcc ttcgcgagaa
     9361 gccgtttaaa ttcttgccat tacagagatg gcactctcaa atagagcgga cggcctgtgg
     9421 aacacgttga tctatacaaa ggaattgtcc gtaacctgcc gaacggttcg atctgggtat
     9481 ttgacagaaa ccttcgttac gtggcggccg ggggagaaat cctcaagcag ctcgggattg
     9541 atgcagatca agtagtaggc aaaacactct gggaagtctg gggtccggag cagacaaagg
     9601 tatccgaacc catttgcaga gcggcgcttg aaggaaagag cacttcagtg gaagagcctt
     9661 ttctgggcag aatttactgg gtgatcaccc atcccattct ggatgaagat ggaatcatcg
     9721 tggggggaat ggtactcaca caggacatca ctgagcacag acaggcggcc ctgctgggaa
     9781 cagaccaaaa gcgcgacctt ctgcttcgcg agcgcgctct aaatgcaatc gacacaggga
     9841 tagtaatcgt ggatgcactg gcgccggatc ttcctattac gtacgtcaac gagggattca
     9901 cgcggctgac tggttacgat gcagaggaag caatcggtag caattgtaga tttttgcagg
     9961 cagaagaccg aaaccaaccc gcacgcgaag agattcgcaa ggccattgca gaagaaagaa
    10021 gaaccagagt catctatcgc aattatcgca aagacggaag catgttctgg aacgaattga
    10081 ctctgtatcc tgtgcgcgaa aacaaccggg tcacacactt catagggatt cagcacgacg
    10141 ttacggatcg aattgaggca gaagaaaccc gcaaatccct ggaagagaac ttgtttcaat
    10201 ctcgcaaaat ggaagcactc ggacttttgt ctgcacgcat tgcgcacgag ttcaacaatc
    10261 ttcttggtgg agtgatgaat tacgcacagt tgctgatcga caggcctgaa gacaaagttc
    10321 agacctacgc gcagaaaatc ctgagtgccg gggtgcgcgg tcgggacgtg accgcacagc
    10381 tcctgtcatt tgcacgcatt gaatcgaaaa acctggcacc atgcgccatc gatcatctgc
    10441 tcgaggaagt aatcgacgta gtcaaagcat cccttccggc gaaaattcaa ttggttactg
    10501 aactgggtgc accagaaaaa atcgtgctgg ccgatcgcac catgctgcac ggagctctct
    10561 tcaatctttg cgctaacgca attgatgcaa tgaaagacac tggccttttg acgattcaat
    10621 catacgcagt agtcggctcc tccattgtaa gaattcgcgt tcaagacacc ggagatggca
    10681 ttgatcccgt gctacttccg cgcatctttg atcctttttt cacaacgaaa ggaaatggca
    10741 aaggcacagg cctcggactc gcttacactc gcgccaccgt cgaatcgcac ggaggcaaaa
    10801 tattcgtgga ttcaacaccg gctcaaggtt ctagctttac aattgaactc cctgtctccg
    10861 accacaaacc agtcgaaaag aacatcgcgt cacccggaac aacgggacag agagaaagaa
    10921 tcctcattat tgaagatgat gaaaacatac tcaaatccgc gactggagcg gctccgcctt
    10981 gcttcaggac ctgcgaggtt acacgaaaga aacgccgctc ctggttattc ccggctatct
    11041 ggatcccgat tcccgcactt ttgtcgaatc cgacgcgaga tctcaatttc tacagaaacc
    11101 ctacctgatc catgaactga tggaagccat cgcgaagagc ctcgaaaaga atccgtgacg
    11161 gccgaaaaac aatcgacaaa ttgggacgtt gcaatacatt gataaatgta gatatgtcaa
    11221 aacgtattca aatcgattca gaactgcaga cgtttctcca ggcggtctca ggggaaagca
    11281 gacagaagat tcttatgtcc ttcttgaatg ggaaggaacg ccccatgagc gacctggtta
    11341 aatcatctga acttggtcaa tccacgattt ctatacaact tgccattctc tgtcgggcag
    11401 gccttctgtt gcgccgcaag cacggaaaag aagtctacta tcgaccggac cgggcacgga
    11461 tcatatctat ggtagataaa ctcagtcact ttttgaaagg atgctgctaa tgcatccctt
    11521 aacgcaaaat ctgcgcaata atacattgat aaatttaaat atataaatac aaaaggtatc
    11581 cgctcaatga aaacagacaa atcgctcaaa cgctccatcg aatgtaccat caaagacttc
    11641 ggcttcccgt ttttactgat gacatataca atgactctgt ggttttttcc gcaaagtgca
    11701 acctttctct tgctggcctt cgcggtggct ggcatggctc ttgagaaatg gatcccgtat
    11761 cacgcagagt ggcaagggat caagactgat tcaaagatcg acgcaatatt cagtggtctc
    11821 acaatcttag tttctgtgat tctcaagagc ggaacgaccg tagtcctttc gcgcgccggt
    11881 atggaatcgc atgtctggcc atcatggcca cttccgatct ccgttctggt tgcccttgtg
    11941 atctctggat tgggtcccta tctgctgcac cgggcggcac acgaatggag cgcgatcatg
    12001 tggcaagtcc acgcggtgca tcatgcgccg gatcgagttt attcattgaa tgcaatgcgc
    12061 acccatccac tcaacgctgc gtggaacgtt ctggcaggtc ttggcccgct gctgctactt
    12121 ggcgcagact cacaaaccat actgattgca ggcagcctca ataacttctt ctccattttc
    12181 aatcatatga atattgattt cagaaaccct atgctttcaa ttttcttcaa cacgtccgag
    12241 ctgcaccgct ggcatcattc caccaaacaa gagttaggtg ataaaaacta cagctccgga
    12301 gcgatcgcac tgtgggacca tgtttttggt acctggagac ttcccgcaga gagatttaag
    12361 agcaatggtg ccgggttgtt tgcagcaaat gattttccgg gatactcttt gtccaaacaa
    12421 ctactgcgcc ctatctgcag atgcgcctga cgcggaaaac ggctcgaact tcgagccaaa
    12481 tgaccgtggg cataaatcgt ttgccaattt cgcacgattc ttgtaattga agaagattcc
    12541 tatgtgcaaa acgattaagc aaaaagtcaa attcaaaaca gacccggaaa ccctttacga
    12601 attgctttct aattctaaga tgcactcgag aatcacaggg caaaaggcag tcatcagcga
    12661 aaaaatcggc ggctcattcg aaactctgtc cggcaaagtt accggaatca atgtagatct
    12721 attgagggga aaaagaatcg tccaggcatg gagaagatca gatttccctg cagggatttt
    12781 ttccatggcg gccgtcacgt ttgaaaaaac gacagacgga ggaacgcaac tcgtgctaac
    12841 tcatcgcggc gtcccaaagg atctcattgc cggaatagaa gacagctggc gtaatcatta
    12901 ctggaagaga
//
LOCUS       DAOQJL010000035        12854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2393_length_12854_cov_4.493320, whole genome shotgun sequence.
ACCESSION   DAOQJL010000035 DAOQJL010000000
VERSION     DAOQJL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2393_length_12854_cov_4.493320"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2640
                     /locus_tag="PLG78_02690"
     CDS             <1..2640
                     /locus_tag="PLG78_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22043.1"
                     /translation="ERRKANPSISTEDLIGELVTLDAPLSGLRRGLEVIPVSEFDRRA
                     PELIRSFRDKTVATRLMQAMEDGPASEHKNAVTRLLSPALPILSRRYAEARTADVASR
                     ERFVTYVVRNPNGEFVEVAFPYLLYRSQIHSTAKFLLLELGAAMLVILFLYPLFFRGS
                     LINPLRTLISGVQRVNSGDLSVSVPVRIEDEIGFLSRSFNNMVTSIRDARDNLEQKIR
                     DRTEDLQKALDKLREADQIKTNFFANVSHELRTPLTLMLAPLESMRAGEVGPLTPTGA
                     SFVDSMLKNGEKLLKLITNLLDYSKLEAGRMQLNYGDAEMVAYVRELVSSFDSAARTR
                     GLFLEVHTAAPQIRAQIDPDKMEKIILNLLSNAFKFTQEGGISLYLSEENGIVQIVVK
                     DTGIGIPEEKLQTIFERFSQVDASASRKYEGTGIGLALARELAILHGGDLSVTSRVGA
                     GSEFVIRFPASGEGKADLQTVTNSRPQTARAGVLAEVELENESSNHDPDTMVELTTQE
                     VQDTRLVLIVEDTHDMRKLLYFFLKPHFRVVTARNGREGLEKARTHRPALIVSDVMMP
                     EMSGYDLLRAVRSEEELKTTPLILLTARADVNMKVEGLDQGADDYLVKPFNSRELLSR
                     IRAQLRIQAMQDEIRTMRDRLLEVNEQLSGKVTEQASEIFLKEKFRNYLPPQLVDAIL
                     SREGDAQVRSTRKKLSIFFSDIVQFTRITGELEPEDIARLLNHYLSEMTKIARKHNGT
                     VDKFIGDAVLIHFGDHGSRGEKQDAIDCVQMAIDMQLRMRELSAEWIEQGFEEPFQIR
                     CGINTGYAAVGNFGSEDRLDYTIIGSNVNLASRIQSNAEPGHILISHATWALARDHFR
                     LEPRDNIAPKGFNRTIPVYAVKF"
     gene            complement(2649..3353)
                     /locus_tag="PLG78_02695"
     CDS             complement(2649..3353)
                     /locus_tag="PLG78_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNE22044.1"
                     /translation="MKAYAGTSGFSYKEWKGPFYPEKIKNDAMLAFYSQQFSTVEINN
                     TFYKMPASKTLETWVEQTPDTFRFSIKASQKITHHQRLKQSDDSVAYLFKQLVHLKDR
                     LGPVLFQLPPYLKKDMERLNSFLEVLPQDVFCAMEFRNAEWFCDEVYDSLRSHNVALA
                     FADGEVEGEQFVATSDRGYVRLRHEQYDEKSIAEWVRRIKSQNWKEAFVYFKHEDAGT
                     GPRLAAEFQRQFQTIG"
     gene            complement(3357..3962)
                     /locus_tag="PLG78_02700"
     CDS             complement(3357..3962)
                     /locus_tag="PLG78_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22045.1"
                     /translation="MRYAAVILCLAMVGCPRNEDDAIMLHLYHKLHVEIERATMGTDI
                     APAYLAAIVSLESHPPGNRSSERFEPYVYQRLVDLKNDNRAFGKIKRESLVFMSDAEI
                     RQLATSYGLTQVMGFHCLELGCSIQDLRGPYHLQWAVGYMMRHYGPHARKRDWNACFR
                     IHNTGLPDGTPARKDYVDQGLMRMQYYQRWMDTRGDVLTFN"
     gene            4037..4618
                     /locus_tag="PLG78_02705"
     CDS             4037..4618
                     /locus_tag="PLG78_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE22046.1"
                     /translation="MLASDSMTALSDPNDWVERHGSLLFSFALTRVRDRATAEDLVQE
                     TLLAALRSRGNFQGRSSESTWMVGILKNKIMDHFRKTSREVDHPLPFENEQLYETTGD
                     WAGHWKEGLTPMHVSPETETEMQELRAKLDSCIQELPGRIASIFTLRELDHAKTEDLC
                     KEFSISPTNLGVILYRARAQLRRCLEKYWMSTQ"
     gene            4615..4893
                     /locus_tag="PLG78_02710"
     CDS             4615..4893
                     /locus_tag="PLG78_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22047.1"
                     /translation="MMANLKEKWILWLARRLPPCEDICRTLSRRLDERIGIGERIKVK
                     LHLWICEYCERFARQLELLQRLNQVEKDAQLSEDAKARIKNAIRSNGG"
     gene            complement(4896..5504)
                     /locus_tag="PLG78_02715"
     CDS             complement(4896..5504)
                     /locus_tag="PLG78_02715"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE22048.1"
                     /translation="MDSRSWTILAWILRTAKKISRIGKILSIIPGMVQDRLQEIQGHI
                     GDIIQRFELTTRKAPQAQQQSASRVAYSGPSFQSHINEVIERQSARHGVSPELIRAVV
                     AQESGGNPGAMSKAGAIGLMQLMPDTARSLGVNPYDMEQNVDGGIRYLKDMARKFDSL
                     DEVLAAYNAGPGAVQKFGGVPPYAETQDYVKKIRHSLRDIKS"
     gene            5489..6355
                     /locus_tag="PLG78_02720"
     CDS             5489..6355
                     /locus_tag="PLG78_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22049.1"
                     /translation="MSENPYTPSFSEVEPSMPDQFDASRAMRDGWEAFKKHLGPAMGI
                     ALVWLIAYVFAANSCFGLFALPHIFAGYSIAALALVRNRPKFELLFSAFETFGPVLVA
                     GLYFALVYLVFIAAIMIFFFVAAFLVGFWGAALQVKDVDQLFKAEGANSPFVWFLIAL
                     GLLAFFLQFYVMARLQLVFPLVLEKKMEATAAFRLSWKLTAPASMRLTGMKVLFDFAL
                     PLLGIALCCVPALFVYPLALAVQGAVLLQYMGPGEAQPVTPQIAQPVPTQMPPPAPPQ
                     DSPERPQNPYGS"
     gene            6477..6623
                     /locus_tag="PLG78_02725"
     CDS             6477..6623
                     /locus_tag="PLG78_02725"
                     /inference="COORDINATES: protein motif:HMM:NF018599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNE22050.1"
                     /translation="MLELRPVCENCSKPLPNGSLEAYICSFECTFYKDCVDRIRTYDH
                     HKTS"
     gene            6601..7119
                     /locus_tag="PLG78_02730"
     CDS             6601..7119
                     /locus_tag="PLG78_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE22051.1"
                     /translation="MITTKRLEIIPCEIRHLEAFARSREELAKMLALRLPPSWPAFPE
                     AYAPPETAAPDPRPWGSYFFLCPALGSLVGSGGFKGKPDSTGTVEIGYEIGKEHWNRG
                     FASEATQGMIDFAFRHEEVKFVTAHTLAEQNASCTVLQKSGLTFIREINDPTDGNIWF
                     WQISKEEHAKRA"
     gene            complement(7147..8055)
                     /locus_tag="PLG78_02735"
     CDS             complement(7147..8055)
                     /locus_tag="PLG78_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNE22052.1"
                     /translation="MASAVERILETMTAQDLAHDFSRLMELPPEMFPLLLITAGAVIF
                     VFAFRHLALRFILPRLGSSASRIKWRRNSAYLAFSILVLLLFPLWLPSLRTVAAFLGI
                     FGAGVILVLKEVIVNVAGWFFIVFRRPFEIGNRITIGAYSGDVLDIRIMDTSLIEVSS
                     RDEGGQSTGRVVHVPNSLVFTVPFANASKEFAFNWNEIRIPISPESDWKRASLLVEKT
                     AAEVVEPISEKDSRIRKSDHEYAIRYQRITPKVYVEFKHGVILLTLRHLVEPRTNRLV
                     SDRFWRILLERFQKEKRIQLHAPMPH"
     gene            8103..8744
                     /locus_tag="PLG78_02740"
     CDS             8103..8744
                     /locus_tag="PLG78_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNE22053.1"
                     /translation="MYTHVRFPKAAALLAITIALATANCGYNSIQSSDEEVKAAWSEV
                     LNQYKRRADLVPNLIKVVERFAKQEKDVFQGVADARSRVGSIQATPELINDEAAFQKF
                     IAAQGQMQGALSRLMAVAENYPQLKSDRAFTDLQAQLEGTENRIAVARGRYIKSVQDY
                     NVLVRQFPNNMTARVFGYTTKPNFTVENEQEISKPPEVDFGTDKPKEPKSENK"
     gene            8741..9238
                     /locus_tag="PLG78_02745"
     CDS             8741..9238
                     /locus_tag="PLG78_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNE22054.1"
                     /translation="MSIGRIIRHIVASPWTLRKMLPADALKRIEDAIGRSENTHRGEI
                     RFAVEVDLNAIQILRGVSPRDRALDVFSQLKVWDTEENTGVLIYLLLADRQIEIVADR
                     GIHKKVGDEAWINIANHIESAFRAGRYEDGVIKGLEEVTKLLSTHFPAKAENRDELSN
                     KPVVL"
     gene            9247..10113
                     /locus_tag="PLG78_02750"
     CDS             9247..10113
                     /locus_tag="PLG78_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNE22055.1"
                     /translation="MRTIFLSAVLLSWFGLSAQELVQIPKLSARVTDLTGTLSGTETS
                     ALEQKLEALETSKGSQIVVVIVPTTKPEAIEQFGIRLAEAWKIGRKKIDDGAILIIAK
                     EDRRMRIEVGYGLEGTLNDATCKRIIDEQIVPHFKKGDFAAGINAGVDAMIAKVQGED
                     LPPPVKGASKGKSSEFGHGPMSGKNAPSFGGLVIGLIVAGSVLTAFVSRLTSGLLVGA
                     GGFLLGWLFLGLSMGLLLAIVSMIATWIFGGRMGGGGGWSSGGSSGWSSGGSSFSGGG
                     GSFGGGGSSGSW"
     gene            10107..10958
                     /locus_tag="PLG78_02755"
     CDS             10107..10958
                     /locus_tag="PLG78_02755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE22056.1"
                     /translation="MVDSQSILLRFSILFQAALILAATVHCEHPKQSALDSIALTSYC
                     DLLAVCQKSDAKFGMIGDSWTDFAGGLPVQIDLHDWLVKRGYTIQASVIAGHTAEAEL
                     SYVRGFDRVIEQSPHIKYMLVCLGGNDLLANNRNYYVNGTDAEMDARIARLDFVHRSI
                     IAQGDAHKIALWGGEGLTWIFSGYDYTNPDMDQSCVRGSLNAGMPLSQAPAQQQRIID
                     RYQTYLNSLPARAPNARSIDLRGTLGGPTQSLVGLKFDCIHPNSAGFYLLAQRYAENL
                     EQMTPEK"
     gene            10955..11761
                     /locus_tag="PLG78_02760"
     CDS             10955..11761
                     /locus_tag="PLG78_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22057.1"
                     /translation="MNSTQFTSRYSFGLFVLLGAAFFATPASAQETYFAVPDGIAMNR
                     ILPAIRFGAEYKSSDLDQHKYYARTQTVDAYGEFAFNENVSIAGYLPWTRKKETNIKS
                     HTRFDNLGLGVHGALPGSTFIPVAGLDLTLPTGSEKVGIGSKRIFNIEPYAGLAFHDG
                     IFSITGLAKYNTQSNRRFREDPEKQDDFERTWLANVGLGLNFEVTDLLLEYQYRYTYD
                     PDPKRKSIHTIAPGLNLKVDHFIFSFSVPCALSKQREFDYGVILRAGMRF"
     gene            11841..>12854
                     /locus_tag="PLG78_02765"
     CDS             11841..>12854
                     /locus_tag="PLG78_02765"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22058.1"
                     /translation="MTFVGNLRRVVGFRTARDYLLGTALFAVIYVTLDLFSDTKPPHW
                     SLLIAYVAYCTVYTLFASWRLVSGAWTPRRWARTTLVFCIPISLFVGYTASTIPHLFL
                     LFGLFIIGIGLPMIGQLRAALWVLTLAVYLGTYLFGLWKLHPEYLFNNKQSVALSTVM
                     FAAITYWLARISWFVEEASKKSTRTLVVTRKQKRELDRISKELRLKDKSLARDLNFAR
                     NFQLNQLPDLEIFNTGGFKSAMHYIALDTVGGDIIDMVRFSPDRIGVFVGDVSGHGVR
                     AAMVAMMARVGFANLCKMSDDPAIVAAALNAFLCRSLENDRSCYMTAIYVIIDRSTMQ
                     MHLT"
BASE COUNT         3286 a         3332 c         3161 g         3075 t
ORIGIN      
        1 gaaaggcgaa aggccaatcc gagcatttcc accgaagatc tgatcggcga gttagtcaca
       61 ctcgatgctc cactttcagg tttgcgccgc ggactcgaag tcattccggt ttctgaattt
      121 gatcgaagag caccagaact catccgttcc ttccgcgaca aaaccgtcgc gacgaggctg
      181 atgcaagcga tggaggacgg cccggcctca gaacataaga atgctgtaac gcgcctgcta
      241 tcgccggctt taccgattct gtcgcgtcgc tatgcggaag cgcgaaccgc cgatgtcgca
      301 tcacgcgagc gatttgtaac gtatgtagtg cgaaacccaa atggagaatt tgtagaagtt
      361 gcatttccat atctattgta tcgatcgcaa atccacagca cggcaaagtt cttactttta
      421 gaactgggtg cggcaatgct ggtaattttg tttctctatc ctcttttctt tcgaggtagc
      481 ctgatcaacc ctctcaggac cctcatatcg ggagtgcagc gcgtgaattc cggcgatctc
      541 tctgtatctg tgcccgtgcg catagaagat gaaatcggat ttctttcgcg ttccttcaat
      601 aacatggtca cttccattag agacgcccga gacaatctcg aacaaaaaat ccgtgatagg
      661 actgaggatc tacagaaggc tctggataag ttgcgtgagg cggatcagat caagaccaat
      721 ttctttgcta acgtatcgca cgaacttcgc actcctctga ccctgatgct agctcccctc
      781 gaaagcatgc gcgcagggga agttggtcct ctgacaccca ctggtgcaag ttttgttgat
      841 tccatgctca agaatggcga aaagctactc aagctcatta caaatctact ggactattcc
      901 aagcttgaag cagggcgaat gcaactcaac tacggcgacg cagaaatggt tgcctacgta
      961 cgcgaactcg tttcttcatt tgattccgca gcccggacga gggggctctt ccttgaggtt
     1021 cataccgcgg cgccgcaaat tcgcgcccag atcgacccgg acaagatgga aaagatcatc
     1081 ctcaatctcc tgagcaatgc attcaaattc acacaagaag gaggaatctc tctatatctt
     1141 tccgaggaaa atggcattgt gcaaatcgta gtcaaggaca ctggaatagg cattccagaa
     1201 gagaagctgc aaacgatttt cgagcgattc agtcaggtgg atgcgtctgc ttcgcgtaaa
     1261 tatgagggca cgggcattgg gcttgctctc gctcgcgagc ttgccatcct tcacggcgga
     1321 gatttgagcg ttacgtcgcg cgttggtgca ggctcggaat ttgtaattcg atttccagct
     1381 tcgggcgaag ggaaagccga cttacaaaca gttacgaact cccgacctca aaccgcaaga
     1441 gcgggagttc ttgcagaagt ggaactcgaa aacgaatcga gcaaccatga cccggatacg
     1501 atggtggagc tcacaaccca ggaagtgcaa gatacaagat tggttctcat cgtagaggac
     1561 acgcacgata tgcggaagtt actctacttc ttcttgaagc cgcacttcag agtagtcaca
     1621 gcgcgcaacg gtcgcgaagg tctggaaaag gcgcgaacac atcggccggc gctcatcgtc
     1681 tctgatgtta tgatgccaga aatgagcggc tacgacttac ttcgagcagt cagatcagaa
     1741 gaagaactga agaccacccc tttaatcctg ctcacagccc gagcggacgt gaatatgaaa
     1801 gtggagggac tcgatcaggg agcggacgat tacctggtca aaccattcaa ctcgcgtgaa
     1861 ttgctttcgc gcattcgcgc ccaactccgg attcaagcta tgcaagatga aatacgaact
     1921 atgcgtgatc gccttctcga agtcaatgaa caactttccg gcaaggttac ggagcaagcg
     1981 tcagagattt tcctgaaaga aaaattcagg aactatctgc ctcctcagct tgtcgacgca
     2041 atcctatccc gtgaagggga tgcgcaggtt cgttctacac gcaagaaact gagcatcttc
     2101 ttctcggaca ttgtgcagtt tacgcgtatc acaggtgaac tcgagccaga agacatcgcg
     2161 cgccttctca atcactatct atccgaaatg acgaagatcg cgcgaaaaca caacggaacc
     2221 gtggacaagt tcatagggga cgctgtacta atccattttg gagatcacgg ttcgcgcggg
     2281 gagaagcaag acgccataga ttgtgtgcag atggctatcg acatgcaact gcgtatgcgt
     2341 gaactatccg cagaatggat tgaacagggt tttgaagaac cgttccagat tcgttgcggc
     2401 attaacactg gttatgcggc agtcggaaat tttggatccg aagaccgcct ggattataca
     2461 atcattggtt caaatgtgaa tctcgccagc cgcattcaaa gcaacgcaga accgggacac
     2521 atactcatat cacacgccac atgggcattg gctcgggatc attttcgcct tgaacctcgc
     2581 gacaatattg cgcccaaggg cttcaaccga accattccag tctatgcagt caaattctag
     2641 gcgcgttctc agccaatcgt ttgaaactgc ctctggaact ccgcggccag tctgggcccg
     2701 gtacctgcgt cctcgtgctt gaaatagaca aatgcctctt tccaattctg gctcttgatt
     2761 ctccgcaccc actctgcaat gctcttttcg tcgtactgtt catgacgaag ccggacgtag
     2821 ccccggtctg acgtggccac gaactgctct ccttctactt ctccatctgc gaatgcaagc
     2881 gccacattgt gcgaacgcag agaatcgtag acttcatcgc agaaccactc ggcatttctg
     2941 aactccatcg cacaaaaaac atcttgaggc agtacttcta aaaatgaatt caatcgttcc
     3001 atgtcctttt tcaaataggg aggaagctgg aagagcacag gacccagacg atctttcaga
     3061 tgcacaagct gcttaaatag gtacgcaacg gaatcatctg attgcttcaa gcgctgatga
     3121 tgcgtgattt tctgcgaagc tttgatagaa aatcggaacg tatcgggagt ctgctccacc
     3181 caggtctcca gggttttcga tgctggcatc ttataaaacg tattgttgat ctctaccgtg
     3241 ctaaactgct gcgagtaaaa cgcgagcatc gcatcgttct tgattttctc cggataaaaa
     3301 gggcccttcc attctttgta agagaaaccg cttgttcctg cgtatgcttt catagttcaa
     3361 ttgaatgtaa gcacatcacc acgagtgtcc atccacctct ggtaatattg cattcgcatc
     3421 aagccctgat ccacgtaatc ttttcgcgct ggcgtcccgt ccggcagtcc agtgttgtga
     3481 atgcgaaagc atgcattcca atctctcttt cgagcgtgtg gtccatagtg acgcatcata
     3541 tagccaacgg cccactgcaa atgatacggc cctctcaaat cttgaatcga acaacccagt
     3601 tccaggcagt gaaatcccat cacctgagtc aaaccgtagc ttgtggccag ctgccgaatc
     3661 tccgcgtcac tcataaagac cagagactct ctcttgatct tcccgaacgc gcgattgtcg
     3721 tttttcaaat caacgagtct ttgatacacg tagggttcaa atctttcgct gcttctattt
     3781 ccgggcggat gcgattccag ggaaacaatg gcagcgaggt atgccggggc aatgtccgtg
     3841 cccatagtgg ccctttcaat ttccacatgc aacttatgat agagatgcaa catgatcgca
     3901 tcgtcttcat tcctgggaca tcctaccatg gccaggcaca gtataacggc cgcgtatcgc
     3961 acagggtatt ggttgcacgc gctaaagcga tgtcaaatga ctttgaacaa ttttttctct
     4021 tgaagttttc tgcctgatgc tcgcatcaga cagcatgact gcactctcag atccgaatga
     4081 ctgggtcgaa agacatggca gtctgctctt cagcttcgct ttgacacgcg ttcgtgatag
     4141 agccacggcg gaagatctgg tacaggaaac acttctcgcc gcattgcgaa gccgcggcaa
     4201 tttccagggt agaagttccg aaagcacctg gatggttggc attcttaaaa acaaaatcat
     4261 ggatcatttt cgaaagactt ctagagaggt agaccatccc ctcccattcg aaaatgaaca
     4321 actctacgaa accacgggcg attgggccgg tcactggaag gaaggcctga cacccatgca
     4381 tgtatctccg gaaactgaaa cggaaatgca ggaactgcgt gcaaaacttg actcctgcat
     4441 tcaggaattg ccagggcgca ttgcctctat cttcactttg cgagagctgg accacgctaa
     4501 aacggaagat ctttgtaagg aattctcgat cagtccaacc aatctaggtg tgatcctcta
     4561 tagagccagg gctcagctca ggcgctgtct ggagaaatac tggatgagca cgcaatgatg
     4621 gccaacctga aagaaaaatg gattctctgg ctcgcccgtc ggctgccccc gtgcgaagac
     4681 atttgcagaa ccctctcccg tcgactggat gaaagaatcg gaatagggga aagaatcaaa
     4741 gtcaaacttc atctgtggat ctgcgaatac tgcgagagat tcgccagaca gctagaattg
     4801 ctacagagac tgaatcaggt agaaaaagac gcccaactat cagaagatgc gaaggcgcga
     4861 atcaagaatg ccatccgatc aaatggcggc taacgttaag acttgatgtc gcgtagagag
     4921 tgacggatct tcttgacata gtcctgagtt tccgcatacg gaggaactcc tccaaatttc
     4981 tgaacagcac caggaccggc attgtacgct gcgagcactt catcgagcga atcgaatttc
     5041 ctggccatat ctttcaagta gcggattcca ccgtctacat tctgttccat gtcgtaggga
     5101 tttacgccca gagagcgagc cgtgtccggc atgagctgca tcaaaccgat cgcacctgct
     5161 ttggacatgg cccctggatt cccgccggat tcctgcgcaa caacagctcg aatgagttcc
     5221 ggagaaacac catggcgagc ggactgtctc tcaatcactt cattgatgtg actctgaaag
     5281 gaaggcccgg agtacgcaac tcttgaggcg gactgctgct gcgcctgggg agccttcctg
     5341 gtcgtgagct caaatcgctg gattatgtct cctatgtgac cctgaatttc ctgaagtcta
     5401 tcctgaacca tccctggaat gatcgacagg atcttcccga tccgtgatat tttttttgct
     5461 gtcctgagaa tccaggccag gatggtccat gagcgagaat ccatatactc catccttttc
     5521 cgaagtagag ccttccatgc cggatcaatt cgacgcgtcg cgagctatgc gcgacggctg
     5581 ggaagcattc aagaagcatc ttggcccggc gatggggatt gcacttgtat ggttgatcgc
     5641 ctacgtattt gccgcgaact cctgctttgg tctatttgct ctgccgcaca ttttcgcagg
     5701 atactcgatt gcagctcttg cacttgtgcg aaatcgccca aaatttgagc ttctcttctc
     5761 tgcgtttgaa acatttggac ctgtactggt cgcaggattg tacttcgctc tggtctacct
     5821 ggttttcatt gcggccatca tgatcttctt cttcgtagcc gcatttctgg ttggtttctg
     5881 gggagcggca cttcaggtaa aagatgtaga ccagctgttc aaagcggaag gagccaattc
     5941 gcctttcgta tggttcctca tcgcactcgg acttctggcg ttctttctac aattctatgt
     6001 aatggccagg ctgcagctcg tctttcctct ggtgctcgaa aagaaaatgg aagcgactgc
     6061 agcatttcgt ctctcatgga aacttacggc gcctgcttcc atgcgcctga ctggtatgaa
     6121 ggtgctcttt gatttcgcgc taccactcct tggaatcgcc ctgtgctgcg tgccggcatt
     6181 gttcgtttac ccgcttgctc ttgcagtgca gggagcagtc ttacttcaat acatgggacc
     6241 tggagaagca caaccggtca cgcctcagat cgcacagcct gtacctactc aaatgccacc
     6301 tcccgctccg ccgcaagatt ctcccgaaag gccgcagaat ccgtatgggt cgtaaggttg
     6361 atcccaaccg tagcgccgcg catacattca ggtgactcaa cgttatgcgc catcgggcgc
     6421 gcgatgaaat ggttgcgtcc atgttctctt cttgtttcct gcaacaggag gcgcgtatgt
     6481 tagagcttcg cccggtttgt gaaaattgca gtaagccact ccccaacggg agcctcgaag
     6541 cttatatatg ctcatttgaa tgcacattct acaaggattg cgtcgaccgg attcgaacct
     6601 atgatcacca caaaacgtct tgaaatcatc ccatgcgaaa tcagacacct ggaagcgttt
     6661 gctcgatcca gggaagaact ggcgaagatg ctcgcactgc gattgccacc atcgtggcca
     6721 gcatttccgg aagcatacgc gccaccggaa acagcggctc cggatccacg cccatgggga
     6781 tcttactttt ttctttgccc cgctcttgga tcactcgttg ggtccggcgg cttcaaagga
     6841 aagccggatt caaccggcac agtggaaatt ggctacgaaa ttggaaaaga acactggaat
     6901 cgcggcttcg cgagcgaagc gacgcagggt atgattgatt tcgcatttcg tcacgaagaa
     6961 gtcaaattcg tcactgcgca cacgctggca gaacaaaacg catcctgcac agtacttcag
     7021 aaatcgggtc taacattcat tcgagaaatc aatgatccaa cagacggaaa catctggttc
     7081 tggcaaatta gcaaagagga acatgcgaag cgcgcctgag ttgaatcagg cctcgcagaa
     7141 gaagaatcaa tgaggcatag gcgcatgcaa ctgaatgcgc ttttcttttt gaaacctctc
     7201 aagtaaaata cgccagaatc gatcagaaac aagacgattc gttcgcggct ctaccagatg
     7261 gcgaagagtc agcaggatca cgccgtgctt gaattcaaca tacaccttgg gagtgatccg
     7321 ttgatagcga atcgcatact catgatccga tttgcgaatt ctagaatcct tttctgaaat
     7381 cggctccact acttccgccg cggttttctc aaccagaagc gatgctcttt tccaatcgct
     7441 ttcaggagaa atgggaatgc gaatttcatt ccagttgaat gcgaattctt tgctggcatt
     7501 tgcgaagggc actgtgaaga cgagtgagtt gggaacgtga accactcgtc ctgtactctg
     7561 tccgccttca tcgcgcgaag aaacttcaat caacgaagta tccatgatgc gaatgtccag
     7621 aacgtctccg gaataagcgc ctatcgtgat tctgtttcct atttcaaacg gacgacgaaa
     7681 tactataaaa aaccatcccg ccacattcac gatcacttct tttaaaacca gaatgacgcc
     7741 agcaccaaag atgcccagga acgcagccac tgtgcgcaaa gacggaagcc acagcggaaa
     7801 caaaagaaga accagaatag agaatgcaag ataggcgcta tttctccgcc acttgatgcg
     7861 cgaagcactc gagccaagcc tgggcaaaat gaagcgaagc gccagatggc gaaacgcgaa
     7921 gacgaaaatc actgcaccgg ccgtaataag aagcaacgga aacatctccg gtggcaactc
     7981 catgagcctc gaaaaatcgt gggccaggtc ctgtgcggtc attgtctcaa gaattctttc
     8041 tacggcggat gccaagattt tttagttgac cctccccctt tcgaggtcac ttcttccaag
     8101 tcatgtatac tcacgtaaga ttcccaaaag cggcagcctt gcttgccatc acgatcgctc
     8161 tggccaccgc caattgcggt tacaacagca tccagagctc tgacgaagaa gtcaaagcag
     8221 cgtggagcga agtgctcaat caatacaaac gccgcgcaga cctcgttccc aatctgatca
     8281 aagtcgtgga aaggttcgcg aaacaggaaa aggacgtttt ccagggagta gcggatgcgc
     8341 gctctcgagt ggggtctatc caggcaactc cagaactcat caacgatgaa gctgcgtttc
     8401 agaaattcat tgcggctcag ggccagatgc aaggggcact ttcacgcctg atggctgtgg
     8461 cagaaaacta ccctcaattg aaatctgacc gcgcattcac ggacctccag gcacagctgg
     8521 aaggaactga aaatagaatc gctgtcgcac gcggccgcta catcaaatcc gttcaggatt
     8581 acaatgttct tgtgcgtcag tttccaaaca atatgactgc acgggtcttt ggatatacta
     8641 ccaagcctaa cttcactgtg gaaaacgaac aagaaatctc taaaccaccg gaagttgatt
     8701 tcggaacaga caaacctaaa gaaccaaaga gcgagaataa gtgagcatcg gaagaatcat
     8761 ccggcacatc gtagcgtccc cgtggacgct acgaaaaatg cttccggcgg acgcgctcaa
     8821 acgcattgaa gatgcgattg gaagatcaga gaacacccac cgtggcgaaa ttcgctttgc
     8881 cgtagaagtg gatctcaacg caatccagat tcttcgcggt gtatctccgc gagatcgcgc
     8941 gctggatgtt ttttcccagc taaaagtctg ggacacagaa gagaacacgg gagtgttgat
     9001 ctatctgctc ctggcagaca gacaaatcga aatcgtagca gatcgcggca tccacaagaa
     9061 ggtgggagac gaagcctgga tcaatatcgc taatcatata gaaagtgcat ttcgcgctgg
     9121 aagatacgaa gacggagtga tcaaaggact ggaagaagtc acgaaattgc tttcgacgca
     9181 tttcccggca aaggcggaaa accgcgacga attgtccaac aagccagtcg tgctctaaaa
     9241 aatccaatgc gcaccatttt tctatccgcc gttttgctct cctggttcgg gctctctgca
     9301 caggaactcg tacagatccc gaaactctcc gcacgcgtca cggatctcac tggaacgtta
     9361 agcggcactg aaacgtctgc actcgaacaa aagctggaag cacttgaaac cagtaagggc
     9421 agtcagatcg tagtggtcat tgtgcccact acaaagccgg aagccattga acaattcggc
     9481 attcgtctcg cagaagcctg gaagattggc agaaagaaaa tagacgatgg tgccattcta
     9541 atcatcgcca aagaagacag aagaatgcgc attgaagtgg gctacggcct cgaaggtacc
     9601 ctaaacgacg cgacctgtaa acgaatcata gacgaacaaa tcgtacctca ctttaagaag
     9661 ggagactttg ctgccggaat caatgctggc gtagatgcaa tgatagcaaa agtccaggga
     9721 gaggatcttc ctccaccggt gaaaggagca tccaaaggaa aatcgtcaga attcggacac
     9781 ggcccgatgt ctggcaaaaa cgccccttcg tttggcggac tcgtaattgg attgatcgta
     9841 gctggatctg tactgaccgc ttttgtaagt cgcctaacgt ctggccttct tgttggcgca
     9901 ggtggattct tgcttggatg gctttttctt ggcttgagta tgggcttact cctggccata
     9961 gtctccatga ttgcaacctg gattttcggc ggacgaatgg gcggcggtgg tggctggtct
    10021 tcgggcggtt cgagcggctg gagctcaggt ggttcaagct tcagtggtgg gggcggcagc
    10081 ttcggcgggg gcggctcttc cggttcatgg tagattcgca gagcattcta cttcgctttt
    10141 ccatattgtt tcaggctgca ttgattctcg ctgcgaccgt tcattgcgaa catccaaaac
    10201 aatcggcttt agattctatc gcgctgactt cctactgcga ccttctcgcc gtatgccaga
    10261 aatcggacgc aaagtttgga atgatcggag acagctggac tgatttcgca ggcggtcttc
    10321 cagtacaaat agatctacat gactggctgg tgaagagagg ttacaccata caggcttctg
    10381 taatcgccgg gcacaccgca gaggcagaat tgagctatgt ccgcggcttt gaccgcgtca
    10441 tcgaacagag tccacacatc aaatacatgc tcgtctgcct gggcggaaac gatctactcg
    10501 ctaacaatcg aaactattac gtgaacggaa ccgatgccga aatggacgcg cgaatcgcac
    10561 gtctggactt tgtacacaga tccatcatcg cacagggaga tgcgcataag atcgcactct
    10621 ggggcggaga agggctaacg tggattttta gcggatacga ttatacaaat ccagacatgg
    10681 atcagtcctg cgtgcgcgga tccctgaatg ccggaatgcc actgtcgcaa gctccggcgc
    10741 aacaacagcg gatcattgat cgctatcaaa cctacctgaa ctcacttccc gcaagagcgc
    10801 ctaacgctcg ttccattgat cttcgcggca ctctgggtgg accgactcaa agcctggttg
    10861 gattgaaatt tgactgcatt caccctaact cagcagggtt ttatcttctt gctcagagat
    10921 acgcagaaaa cctggaacag atgacgccag aaaaatgaat tctacacagt ttacatccag
    10981 atactcattc ggtcttttcg ttcttctggg ggccgctttt ttcgctactc cggcttctgc
    11041 gcaggaaacg tactttgctg tgccagacgg aatcgcaatg aaccgcattc tacctgccat
    11101 tcgatttggc gctgaataca aatccagtga tctggatcag cacaagtact acgcaagaac
    11161 gcaaacagtg gacgcatatg gcgaattcgc attcaatgaa aatgtttcta tagcgggtta
    11221 tcttccctgg actagaaaga aggagacaaa catcaagtct cacactcgct tcgacaatct
    11281 cggcctggga gttcacggag cattgcccgg atccactttc atacctgtgg ccggactcga
    11341 tctgactcta cccacaggga gcgaaaaagt aggaattgga agtaagcgca tcttcaacat
    11401 cgagccgtac gcgggccttg cattccatga tggaattttt tccattacgg gccttgcgaa
    11461 atacaacaca cagagcaacc gtcggttcag agaggatccg gagaaacagg atgactttga
    11521 acgaacctgg cttgccaatg ttggacttgg actgaacttc gaggtgacgg atctgctcct
    11581 ggaatatcaa tatcgttaca cctacgatcc ggacccgaaa agaaagagca ttcatacaat
    11641 tgcgcccgga ctgaacctga aagtggatca tttcattttt agtttctctg tgccgtgtgc
    11701 actttccaaa cagagggaat tcgactacgg cgtgattctg cgcgccggga tgcgttttta
    11761 gaatccgtcc cgcgaaagcg gaacacacca ttgggcagaa tttctgctga catatataag
    11821 cactgacttt ccctctgctc gtgacgtttg tcggaaatct aaggcgagtg gttggatttc
    11881 gcactgctcg ggactatctc ctgggcaccg cgctctttgc ggtgatttat gttaccctgg
    11941 atctattttc agacacgaaa ccaccgcact ggtccttgtt gatcgcctat gttgcatact
    12001 gcactgttta tacgctcttc gcatcgtggc gtctggtttc cggcgcctgg actcctagaa
    12061 gatgggcgcg cacaacgctg gtcttctgca ttcccatttc tctgttcgta ggttatactg
    12121 cgtcaaccat cccccacctc ttccttttgt ttggtctttt catcatagga atcggtctgc
    12181 ctatgatcgg ccagcttcgc gctgctctct gggttctgac tcttgccgtc tatctgggaa
    12241 cgtatctctt tggcttgtgg aagcttcacc cggaatactt gttcaacaac aagcaatcag
    12301 tggctctctc tacggtgatg tttgcggcca tcacgtactg gcttgcgcgg atctcgtggt
    12361 ttgtggaaga ggcctcgaaa aaaagtacgc gcacactcgt agtgacgcga aagcaaaaac
    12421 gagaactcga cagaatcagc aaagaactga gactcaaaga taagagcctc gcccgcgatt
    12481 tgaacttcgc acggaacttt cagctcaacc aacttccaga tctggaaatc ttcaatacag
    12541 gtggattcaa aagtgccatg cactacattg ctctcgacac ggtgggcggt gacatcatcg
    12601 acatggttcg cttctctccc gatcgcatcg gagtttttgt gggcgatgtt tccggtcacg
    12661 gagttcgagc ggcgatggtg gccatgatgg cgcgagttgg atttgcgaat ctgtgtaaga
    12721 tgagcgatga ccccgccatc gtcgcggccg cactcaatgc ctttttatgc agatctctcg
    12781 aaaacgatcg gtcctgttac atgactgcga tctatgtaat tattgatcga tccacgatgc
    12841 aaatgcatct gacg
//
LOCUS       DAOQJL010000036        12742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2422_length_12742_cov_4.752975, whole genome shotgun sequence.
ACCESSION   DAOQJL010000036 DAOQJL010000000
VERSION     DAOQJL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_12742_cov_4.752975"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLG78_02770"
     CDS             complement(<1..811)
                     /locus_tag="PLG78_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNE22059.1"
                     /translation="MKSEPGLPVLTGIGIVSPIGIGRSEFWAGLTEGRTGVRNISLFD
                     ASSFPSKIAAEVIGFDASPYLSRRELKSYPRQTQFALVGLMLALGDAKLAALDPYRTD
                     VVLGAAQISFYAIEEEIRKGSHALREHEPELDPFGVLKTTLSIPASAIAFRSGVKGYV
                     STLTSACTSGIDAIGAAADRIRHGQADAVITGGVDTPITRIILNAFCAARMLSTRNDE
                     PQSALAPFDRSHTKSVLGEGAAILILEDKRRAIARGASIYCELGRFAQEPEN"
     gene            1008..2000
                     /locus_tag="PLG78_02775"
     CDS             1008..2000
                     /locus_tag="PLG78_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22060.1"
                     /translation="MVTVDRPELARGGSQMILCHSVANKQSLGRKKTKKGSLLAIMMM
                     VHALAVPSAWAEDFETRMRAIQSTLDKGLVEENPQLVSEGLVKLQNEKPSPDSENYDA
                     WVKFVKEVDAKLVRFKITSRETTVVEVKGATPTQTVTRKIIESASQDCNRSLHTISFF
                     GLYYKPCNFGIAVPGITAIAGRGSRSYVTKAQVNGLGIVQVMERSSYKPLTFLSATAY
                     YKWANSDVPFFWGIPVGVGLDKQAASSPPLMWGFSAGLVIDDTKFFAVTYGEFYDPGT
                     TRIAFPFYEGAPNPITGLTTLNATSLTQNGLISVPLEKVRGIYRGLGFSFNYKF"
     gene            complement(2065..2427)
                     /locus_tag="PLG78_02780"
     CDS             complement(2065..2427)
                     /locus_tag="PLG78_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22061.1"
                     /translation="MSDSEKAGSLYAKTMSHDTAGNDLRLRVAEIYGTYEQMRERTEI
                     MAASFLSDCYYGDRPARGAWLADITDFRSILCIGHDTEHSPICIDFAHGAKVILWDGY
                     AWREVAPGFAGFLRLFVG"
     gene            complement(2460..3698)
                     /locus_tag="PLG78_02785"
     CDS             complement(2460..3698)
                     /locus_tag="PLG78_02785"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22062.1"
                     /translation="MKEHSLEHRLFSTISLLNGVANIGGSFAYLLQGGSGLLQFAINA
                     GTGVLFLGLYYLSRFRGIYYILYWPFNLLILGFLATLWFPNGGSHGGSSYYLIPALMI
                     ATILARSGRTIVLVFLLYAAVAVALLFIEHQRPDLIAMPAVEDRFLDIVLNYPFVQIF
                     SSGLVVVLSRNVHAEREKSDKLLLNILPQEIAEELKKTDQVAPQSYDQVTVLFTDLVG
                     FTRIAENLPPQDLLRELDSCFSSFDRIIKKHKIEKIKTIGDSYMCAGGVPHRNKTNAI
                     DCVLAALNMITYMKRLKADREAHGLPCWEIRLGIHTGHLVAGVIGKEKFAYDIWGDTV
                     NLASRMESSGVPMQVNISRATYELVSNLFDCEFRGAVQAKNKGEIDMYLVRGVKAEFK
                     GEDGKLNRKFVEAYKEVGAA"
     gene            3769..4317
                     /locus_tag="PLG78_02790"
     CDS             3769..4317
                     /locus_tag="PLG78_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE22063.1"
                     /translation="MITAVGKTNQRKEQILSRGLAVTLHILDQGRELLAKLTQEDYSA
                     RSPLGASVGSHMRHILDFYRCFLRDEPAGSIDYDLRERNSESERLLDLARRELELVRE
                     RLRGLRWTENEPVQVKVEASHPVEFVTSTREREIQFLISHTVHHFALISYILAERGFA
                     VPADFGIAPSTLEFLRAESLAG"
     gene            4307..5959
                     /locus_tag="PLG78_02795"
     CDS             4307..5959
                     /locus_tag="PLG78_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE22064.1"
                     /translation="MLADGLAYVLIALSTFVSEDLTCIATGMAAATHKVALVPGIIAC
                     ASGIFAGDLLLFAAGRILGRPAVKIRPLKWFVTERKIEESSRWFAAKGHWLILTSRFI
                     PGTRLPTYFAAGLLKVNLVEFLFWFALAALLWTPLIVSIAYFLGSQAHALFEESHILP
                     ILAVLAAALYFSVHLLLSLVTFRGRRMLAGKWYRMRWEFWPSWAAYPPIIFYCIYLAI
                     RFKGPLLFTASNPGIESSGVIGESKSRILDHLPPDSVAKYKLIPSMKEPSGQAANERA
                     SIALSFMAEHALTFPIVIKPDQGQRGSGVQIVRSEQELTIALKNPKALIIQEYISGLE
                     FGILYARMPDEENGRILSITRKTMPRLVGDGKQTLEEMILRDHRAVALADHYCSANQS
                     RLTYIPKEGESIQLVELGTHARGSIFLDGADLQTTALEQEIDRISKCFRGFYLGRYDI
                     RVKDESALMRADGIRILELNGVTSEPTHIYDPAVSVIHAYRALFEQWRLAYAIGASNR
                     QKGFQPMTVREMISLLTSAIREPETESNPDESKEPQQQTTRL"
     gene            complement(5886..6278)
                     /locus_tag="PLG78_02800"
     CDS             complement(5886..6278)
                     /locus_tag="PLG78_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE22065.1"
                     /translation="MIKLSWLLQILCAVILAQTLYFKFSGAPESVWIFSRLGMEPWGR
                     YLTGGLELVAVLLLLVPRTIWMGATLAGGLMLGAIISHLAVLGVVVIGPDGRGDGGLL
                     FSLAAIDGLFAAAVLWIRRDSIPFLARE"
     gene            6337..6867
                     /locus_tag="PLG78_02805"
     CDS             6337..6867
                     /locus_tag="PLG78_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNE22066.1"
                     /translation="MADKWKPELTQPSPARLYAACYFDDGEPYLTAKKKLESAFGKAD
                     YESHSVPTELLMPLYTNLARKTMRILSFQRQVAREEIVDIRRKTLAVESGLQQLGRPL
                     VEIDPGYVTEFTSVRTSLKDDFHHIYLYGGIFAQSLYYYESGSFRPFVHTPDLYRYES
                     VISIFNDLRLAHVTDR"
     gene            6851..7438
                     /locus_tag="PLG78_02810"
     CDS             6851..7438
                     /locus_tag="PLG78_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22067.1"
                     /translation="MLRIDRTPYRLVRRFAYCVATLAVLIPCACGGPIKLEKAFLCDD
                     FAEGKCTAPPPEGGIYRFSLPAEKTKTWFDFAYHMYFHSRQTPGMRVNFSRGLNTQEE
                     STRTGAHCVYKISKGDKFAEDHMEGLRWDENGSGLWCFDYLGTMLVKFHKKYGTIQTK
                     PTPDFFPVTLNIKLEGGPVSAEQQETIYLDWTAAP"
     gene            7435..8040
                     /locus_tag="PLG78_02815"
     CDS             7435..8040
                     /locus_tag="PLG78_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNE22068.1"
                     /translation="MSEEHKLPEGLMEVKISDVSITNVGFAIFMKPVGSTSNKVVPIF
                     IGPLETYSISSALDGVTPPRPNTHDLIINMISELESRILHVIINDIIGNIFYARIVIQ
                     SDDGIVEIDARPSDSVAIAIRAKCPIYMHDKVYQEAGVVIGDEAKSEASENEDEETPA
                     STMTQSPLEALKTRLQKAVEQERFEEAAELRDQIERLQREN"
     gene            8048..10090
                     /gene="recG"
                     /locus_tag="PLG78_02820"
     CDS             8048..10090
                     /gene="recG"
                     /locus_tag="PLG78_02820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNE22069.1"
                     /translation="MAELTSIAGVGPARAELLLAAGIKSLEDLLYFFPRRYLDRTFAE
                     STVFRAGIETTLIVTVQTAYLAHGRMSRLVVQTRTMQGEPLSLVFFRGAQYFRRVFVK
                     DATFVVSGKLEFYGGMQMVHPDFEELDSEDAETIHVGRIVPVYPGSEALKKKGLDSRG
                     FRRILHSVLESSVTLPDLLPDGLVKSARVLDRMQSLHAIHFPQNKEELALARHTLKFE
                     ELFLFSAHMKQKRDERLREPRMLWPLAPGKSKQFDSLRARLPFQLTTEQDKAILAILK
                     EGTTDHAFAFLLQGDVGSGKTLVALASMLHYTENRVQCAMLAPTEVLARQHFRTFVDL
                     LGLSGVQVDLLISADSKKSKAQVLDRLARGETDIVIGTHSLLEPEVVFESLGLVIIDE
                     QHRFGVEQRESLRRKGKNPDVIGMTATPIPRTLCLTEFADLSLVTLKEKPAGRVPIKT
                     IRLAEDRRPGLYKSIRNHVSNGRQCYIVYPVIEESEKLDVRAATDAYEELRTSIFPDL
                     KVELLHGKMKTEDKDRVFREFRAGRVQILVSTTVIEVGVDVANATIMVIEHADRFGIS
                     QLHQLRGRVGRGSEESFCILMAGETTEDGEERLNAIVNSDNGFELAEVDLKIRGPGEI
                     LGKRQHGVDGLRIASLLEDRKLVEESFAAANEWTDLPPGALEFIKNRFSSAEQSLS"
     gene            10096..11352
                     /locus_tag="PLG78_02825"
     CDS             10096..11352
                     /locus_tag="PLG78_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22070.1"
                     /translation="MQTIPAPSRDIVSFFRALANIGVKQTMSPPEKKRVVLSNQLFLI
                     AAGVTLSFLPLGLALGTSAIGTVTSLIDTLLLAGCFFLNYRGQYKMARVMGLVVGNSL
                     TFFWVLALGNQMYPELFYFTLAAAPLLFIGPDQKKSLYVLSLLSVVLFFVSHELKAKI
                     APMILIPGDIALLAGYSTGVLSFITLLSILYYFHRGTERVESELDSERKKSDRLLLNI
                     LPEPIADRLKAGERLIADRFDRATVLFADIVNFTPMSGSMPADEVVSILSRIFSAFDM
                     LCEKNGMEKIKTIGDSYMAAAGLPEPDENHMQSAARMALDMQQMILEMARREGMDLSI
                     RIGIHSGPVVAGVIGEKKFIYDLWGDTVNLASRMESHGIPGQIQVSQHIYETLHSTFT
                     FETRGLIEVKGKGPMQTYFLTGSTRD"
     gene            11458..>12742
                     /locus_tag="PLG78_02830"
     CDS             11458..>12742
                     /locus_tag="PLG78_02830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22071.1"
                     /translation="MLLGTAFALYFQDRRNTLNRLLALQLILIAIWQYTGFLHALLSA
                     GPPLVRPLQIVAGVVAAYVFFVLGRHLGRLRAGKWEAAGAAWIFLIFIYYMVRILIPD
                     SGNAAEFVLVDGRPVYGPTPAYVTYTSTLSAFYGVGIFHILKARRREEDTVMRKRMLL
                     IVLTIGAAAMSGIIFINTFYILNIQMPFPIERVIMVSALLFLGYSVLGERAFSNENLI
                     RLVREKEKAIRDRNRVIESELDLARMIHSRLFPDKPPRIPGYDIQGACISTDKVGGDF
                     YDFYTRLENLGIFIADVSGHGIPAAFIASCTKMAFNYSAGHAENGTQLLKKMDVAIAR
                     RAVQSMYVTAVYAEIDYASRQLSYCTAGHGPILLHRRSDNTLQHLKAKGSPLGLMTGK
                     PFEMRTLQLIPGDRLVFFTDGLTESINHERKPYTEE"
BASE COUNT         3361 a         3233 c         3098 g         3050 t
ORIGIN      
        1 tattctctgg ctcctgagca aaacgaccga gttcacaata gatggaagca ccgcgagcga
       61 tcgcacgacg cttgtcctca agaatcagaa tggccgctcc ttctcccaga acggatttgg
      121 tgtgactacg atcaaaagga gccagagcgc tttgcggttc atcattgcga gtagaaagca
      181 ttcttgccgc acaaaatgca ttgagtatga ttctggtaat gggtgtgtcc actcctcctg
      241 tgatgactgc atctgcctgt ccatgacgaa tgcgatctgc agccgctccg attgcgtcga
      301 tcccactcgt gcacgcagaa gtcagggtgg acacgtatcc cttcactcca ctgcgaaatg
      361 caatcgcaga agcaggaatg ctcagagtgg tctttagcac accaaatgga tcgagttcag
      421 gctcgtgttc tcgcaatgcg tgactgcctt tcctgatttc ttcttctatt gcatagaagg
      481 aaatctgtgc tgcgcccagc actacatccg ttctatacgg atcgagcgcg gcgagcttcg
      541 cgtccccgag agcaagcatc agcccgacca gtgcaaactg agtttgcctt ggataggact
      601 tcaattcgcg gcgagataga tacggagatg cgtcaaagcc aatcacttcc gccgcgattt
      661 tgcttgggaa agaagatgca tcgaaaagag atatatttcg aaccccggtg cgcccctctg
      721 tcaggccggc ccagaattcc gaacggccaa tcccgatggg ggaaacaatt ccaatcccag
      781 tcaaaaccgg caatcctggc tcagatttca tggtagacag taaatggccc tgttcttgcc
      841 atccgggtca acaatatcgc tgaggaatta gtaaaaaaaa tatatcgttt tttgaataat
      901 tattgaccga agtttgtaaa tgtcgatatg ttgggcgcgc tcttgaaaag agcatgagaa
      961 cgcaatgaac cttgcacctc ggatttcaac gatgtcatat attcgaaatg gtaactgtcg
     1021 atagaccgga gctggccaga ggaggatccc agatgattct atgtcacagc gtagcgaaca
     1081 agcagagctt gggtcgcaaa aagaccaaga aaggcagctt gttggcgatc atgatgatgg
     1141 tccatgcttt ggctgtaccg tcggcctggg cggaggattt cgagacaagg atgcgagcta
     1201 tacagagtac cctcgataaa ggcctcgtgg aagaaaaccc tcagcttgtg agtgaaggat
     1261 tggtgaaatt gcagaacgag aagccttccc ctgatagcga aaactacgat gcttgggtaa
     1321 aattcgtgaa ggaagtggac gcgaaactcg ttcgcttcaa gattactagt agggaaacca
     1381 ccgtcgtcga agtcaaaggc gcgactccaa cccaaaccgt gacccgtaag attatcgaaa
     1441 gtgcgtctca ggactgcaac cgatctctgc acacaatcag cttttttgga ttgtactaca
     1501 agccttgtaa cttcggcatt gcggtgcccg gaataacagc aatcgcgggt cgcgggtcaa
     1561 gatcctacgt gaccaaggct caggtaaatg gactcggaat cgtccaagtg atggaaagga
     1621 gtagctacaa accattgaca ttcttatccg caacggctta ctataaatgg gcaaattcgg
     1681 acgttccctt cttctggggt attcccgtgg gagttggcct ggacaagcag gctgcttcta
     1741 gcccgcctct aatgtggggc ttctcggccg gcctcgtgat cgatgacacg aagttctttg
     1801 ctgtgactta cggcgagttt tatgaccctg gtacaacacg tattgctttt ccattttatg
     1861 aaggtgctcc gaatccgatc acgggcctga ccacattgaa tgcgacttcc ttgactcaga
     1921 atggcctaat atcggtgcca ctggaaaaag ttcgcggtat ttatcgcgga ctcggcttca
     1981 gtttcaacta taagttctaa gaaaaaggca cccggtgaca cggcgcggcg gcaaccgcgc
     2041 aagaaagcgt tcgtttgtgg gcccctaccc cacaaacaat cgcaaaaatc cagcaaaccc
     2101 cggggccacc tctctccatg cgtatccgtc ccagagaatc actttggctc cgtgcgcaaa
     2161 atcgatgcag atgggagaat gctctgtgtc gtgaccaatg cacaagatag aacgaaagtc
     2221 tgtgatgtct gcgagccagg ctcctcgcgc agggcgatct ccatagtagc aatcagaaag
     2281 aaaagaagca gccatgattt cagtgcgttc gcgcatctgt tcgtaggtac catagatttc
     2341 cgcaacgcgc aacctcagat catttccagc tgtgtcatgg ctcatggtct ttgcataaag
     2401 cgaccctgcc ttttctgaat cagacatcaa gtcctgcaat tcagacggca gggtatgaat
     2461 caagcagcgc ctacttcttt gtatgcttcc acaaactttc ggttcagctt tccgtcttct
     2521 cctttgaact ctgcttttac accgcgaacc aggtacatgt cgatttcgcc tttgttcttt
     2581 gcctgaaccg ctccgcgaaa ctcacaatca aacaaattgc taaccagttc atacgttgct
     2641 ctggaaatat tcacctgcat gggaacaccg gaactctcca tgcgagaagc gagattcaca
     2701 gtatctcccc agatatcgta tgcgaatttc tctttcccaa tgactccggc tacaagatga
     2761 cccgtgtgaa tccccagcct tatttcccag caaggcaggc catgagcttc gcgatccgct
     2821 ttcaagcgtt tcatatacgt aatcatattg agcgctgcca gtacacagtc gatcgcgttt
     2881 gtcttgttgc gatgcggcac gccgcccgcg cacatatagc tgtctccgat cgttttgatt
     2941 ttctcaatct tgtgtttctt tataattcta tcaaaagaag aaaagcacga atcgagctct
     3001 ctgagtaaat cctgtggcgg caaattctcc gcgatgcgag taaacccaac aagatccgta
     3061 aacaacacag tcacctgatc atagctctga ggtgctacct gatctgtttt cttgagttcc
     3121 tctgcaatct cctggggaag gatattcaaa agcagcttgt ctgacttttc tctttccgcg
     3181 tgaacgttcc tagataggac cacgaccaga ccggaactga aaatctgcac aaaaggatag
     3241 ttcaggacaa tgtccaggaa acgatcttcc acagctggca tggcaatcaa atccggacgc
     3301 tggtgttcaa tgaacaaaag cgcaacggcc acagccgcgt acaaaagaaa aacaaggacg
     3361 atggttcttc cgcttcgagc gagaatcgtg gcaatcatga gagcgggaat caagtagtag
     3421 ctcgaacctc cgtgcgatcc accgtttgga aaccacaaag tagccagaaa tcccaggatg
     3481 agcaagttaa agggccagta caaaatatag tagatgccgc gaaatctgga cagatagtac
     3541 agccccagaa acaaaacgcc tgtgcctgca ttgatcgcaa actgcagcag tccgcttccc
     3601 ccttgtagaa ggtaggcaaa cgagccccca atgttggcca ctccattgag caaagagata
     3661 gtggaaaaaa gtctatgctc cagggaatgt tccttcacat ttcccaccag cctgtaaaaa
     3721 atccggatta gcagttgcac cataacagtt cgtcgcggaa acactgtaat gattactgca
     3781 gttggcaaga ccaatcaaag aaaggagcag attttgtccc gtggactggc tgtaacgttg
     3841 cacatacttg atcaaggacg cgaacttctg gcgaaactca cgcaggagga ctattcggct
     3901 cgttctccgc tgggtgcatc cgtgggcagt cacatgcgac acattctgga tttttaccgc
     3961 tgcttcctca gggacgagcc agccggaagc atcgactacg atctgagaga aagaaactcg
     4021 gaatcagaac gactcctcga tctcgcgcgg cgagagctgg aactggtacg cgaaagacta
     4081 cgagggctcc ggtggaccga aaacgagcct gtgcaggtaa aagtagaagc ttcgcatcca
     4141 gtcgaatttg tcacgtccac gagagagcgc gaaatccaat ttctgatttc gcacacagtt
     4201 catcatttcg cactgatttc ctacatcctg gcggagagag gatttgctgt tccggctgac
     4261 tttgggattg caccttccac cctcgaattc ttgagagcag agagtcttgc tggctgacgg
     4321 actcgcctac gtactgattg cactctccac cttcgtaagc gaagatctaa catgcattgc
     4381 cacgggaatg gcggctgcga cacacaaagt cgccctggtt ccaggaatca tcgcatgtgc
     4441 cagcggaata ttcgcagggg acttgctact atttgcagca ggcagaattc tgggtagacc
     4501 tgccgtaaaa atccgtcctc tgaagtggtt cgtcacggag cgtaagatag aagaaagctc
     4561 caggtggttt gctgcaaaag gacactggtt gattctcaca tcgcgcttca ttccaggaac
     4621 caggctaccc acgtactttg ccgctggcct tctgaaagtg aatctggtgg agttcctgtt
     4681 ctggttcgca cttgcggctt tactctggac gcctctgatc gtaagtattg catactttct
     4741 aggatcgcag gcgcacgcac tcttcgagga atctcacata cttcccatac tcgcagtgct
     4801 cgccgctgcc ctgtatttct ctgttcatct attgctctct cttgtcacat ttcgcggaag
     4861 gcgaatgctt gccggaaaat ggtacaggat gcgctgggaa ttctggccat cctgggctgc
     4921 ctatccgccc atcatatttt actgcatcta tcttgcgatt cgatttaaag gccctctttt
     4981 gttcactgcg tcgaatccgg gaatagaaag ttccggtgtc atcggtgaat ccaaatcgcg
     5041 catcctggac catcttcccc cggattcagt tgcaaaatat aaattgattc cttccatgaa
     5101 ggagccgtcc gggcaagcgg ccaacgagcg cgcgtccatc gctcttagtt tcatggcaga
     5161 gcacgcactc acttttccaa ttgtgatcaa gccagatcaa ggacaaagag gcagcggagt
     5221 gcaaatcgtt cgaagcgaac aagagcttac gattgcactg aaaaacccga aggcgttgat
     5281 catacaggaa tacatttccg gcttagaatt tgggattcta tatgcgcgca tgccagatga
     5341 agaaaacggt cgaatactct cgattacgcg aaagaccatg ccgcgtcttg taggagatgg
     5401 aaaacaaact cttgaagaaa tgattctgcg cgaccatcga gccgtagcac tcgcagatca
     5461 ctattgcagc gcaaaccaga gtcgcctaac gtatattccc aaagagggag aatccattca
     5521 actcgtagag ctgggcactc atgcgcgtgg ttctatcttt ctcgatggtg ctgacctcca
     5581 gacgactgcc ctggaacaag aaatcgatcg aatcagcaag tgctttcgcg gattctacct
     5641 gggtagatac gacattcgtg tgaaagacga atctgcactc atgcgcgcag atggaattcg
     5701 tatactggaa ctcaacggag tgacttcaga acccactcac atttacgatc cagcagtctc
     5761 ggtgatccac gcatatcgcg cactttttga gcaatggagg cttgcctacg caattggagc
     5821 ttccaatcgc caaaaaggat tccagccaat gaccgtgcgc gaaatgatct ccctgctaac
     5881 cagtgctatt cgcgagccag aaacggaatc gaatcccgac gaatccaaag aaccgcagca
     5941 gcaaacaacc cgtctatagc ggccagggaa aaaagaagtc caccatctcc gcgaccgtcc
     6001 ggcccgatga caactacgcc gagtacagcc agatgactga tgatggcgcc aagcatcaat
     6061 ccgccggcga gagtcgctcc catccagatg gttcgcggca caagcaaaag caagactgca
     6121 accaattcca gcccgccggt caaatatcta ccccacggtt ccattcctag cctggaaaaa
     6181 atccagacag attcaggcgc tcccgagaat ttaaagtaca gagtctgcgc gaggatgacg
     6241 gcgcagagga tttgaagcag ccagctcaat ttgatcattc aatccctccc cggaattagt
     6301 tgtttacgcg ccacagttat tacacaaatc ctcaccatgg cggataagtg gaagccagag
     6361 ctgactcagc cgtcaccggc cagactttac gcagcctgct attttgacga cggagaaccc
     6421 tacctcaccg cgaagaagaa gctggaatcc gcatttggca aggccgatta cgaaagtcat
     6481 tctgtcccga ccgaactcct gatgcccctc tatacaaatc tagccaggaa gactatgcgc
     6541 attttgagtt ttcaaaggca ggtcgcgcgc gaggaaatcg tggatatcag aagaaagacc
     6601 ctggctgtag aaagcggact gcaacaactg ggtcgaccgc tggttgaaat agacccggga
     6661 tatgtcacag aattcacaag tgtgcgcact tctttgaagg atgactttca ccacatttac
     6721 ctgtacggtg ggattttcgc gcagagtctc tactattatg agtctggttc cttcaggccg
     6781 ttcgttcata cgccggacct gtatcgatac gaatctgtta tatctatttt caacgatttg
     6841 aggctggctc atgttacgga tagatagaac tccgtatcgt ctcgtgcgcc gattcgcgta
     6901 ctgtgtagcc actctggcag tcctgatccc atgtgcctgc ggaggtccta tcaaattaga
     6961 aaaagcgttt ctgtgcgatg atttcgcgga aggcaaatgc acagctcccc ctccagaagg
     7021 agggatctat cgattcagtc tgccggcaga aaaaaccaaa acctggtttg acttcgcata
     7081 tcatatgtat tttcattcca gacagactcc gggaatgcgc gtgaactttt cgcgcggctt
     7141 gaacacccag gaagaaagca cgcgaaccgg agcgcactgc gtgtacaaaa tctcaaaggg
     7201 agacaagttt gcagaagatc acatggaagg attgcgctgg gatgaaaacg gttcaggact
     7261 ctggtgcttt gactacctgg gaaccatgct tgtgaagttt cacaaaaaat acgggaccat
     7321 ccagacaaaa cccactccag atttcttccc cgttacattg aacatcaaac tggaaggcgg
     7381 acctgtttcc gcagaacaac aggaaacaat ctacctcgac tggacggcag cgccatgagc
     7441 gaagaacaca aactccctga aggcctcatg gaagtcaaaa tctcagacgt gtccatcacg
     7501 aacgtcggat tcgccatttt catgaaacca gttggatcta catccaacaa ggtggttccc
     7561 atctttattg ggcctctcga aacgtattct atttccagtg ctctcgatgg ggtaactcca
     7621 cctcgtccga acactcatga tctcatcatc aacatgattt cagaattgga atcgcgcata
     7681 ctgcacgtga tcatcaatga tatcatcgga aacattttct atgccagaat cgtgatccag
     7741 tcagacgatg ggatcgtaga aattgatgct cgtccttccg attcagtggc cattgccatt
     7801 cgcgcgaaat gccccatcta tatgcacgac aaagtgtatc aagaggctgg cgtggtgatc
     7861 ggtgatgaag ccaaaagcga agcatccgaa aacgaagacg aagaaacacc cgcttccaca
     7921 atgacgcaat ctccgctaga ggcattgaaa acaagactgc aaaaagcggt tgagcaggaa
     7981 cgatttgaag aagcagcaga gctccgggac cagatcgaaa ggctccagcg cgaaaactaa
     8041 tccagccctg gcggagctca cgtcgattgc aggcgttggc ccggctcgcg cggagctatt
     8101 gctggctgca ggaattaaat cactcgaaga tctactctat ttctttccac gtcgctacct
     8161 ggatcgcaca ttcgcagaaa gtaccgtatt tcgcgcggga atagaaacca ctctgattgt
     8221 caccgtgcaa accgcgtatc tggcacatgg cagaatgtcc agactggttg tgcaaaccag
     8281 aaccatgcaa ggagagcctt tgtctctcgt attctttcga ggcgcgcaat actttcgtcg
     8341 agtgttcgtg aaagatgcaa cgttcgtggt ctcgggcaaa cttgaatttt acggtgggat
     8401 gcaaatggtg catcccgatt ttgaagaact ggattccgaa gatgcagaaa ccatccacgt
     8461 tggaagaatc gtacctgttt atcctggttc agaagcactc aagaagaaag gattggatag
     8521 cagaggtttc agaaggattc tacattctgt cctggaaagt tcggtcactc tgccagatct
     8581 gctcccagat ggtctggtca aatctgctcg tgtgctcgat agaatgcaat ccctgcatgc
     8641 gattcatttt ccacagaaca aagaggagct ggctcttgcc agacacactc tgaagtttga
     8701 ggagctgttt ctcttttcag cgcatatgaa acagaaaaga gatgaaagat tgcgcgaacc
     8761 tagaatgctc tggccactgg ctcctggaaa atcaaagcag tttgactctc ttcgcgccag
     8821 gcttcctttc cagcttacga cggagcaaga caaagcaatc cttgcgattt tgaaagaagg
     8881 aaccacagat cacgcattcg ccttcctgct gcaaggtgat gttggttcag gaaagactct
     8941 ggtcgctctg gcaagcatgc ttcactatac agaaaacaga gtgcagtgcg ctatgctcgc
     9001 tcccacagaa gttctagcac gccaacattt tcgtacgttc gtagatttac ttggactttc
     9061 aggagttcaa gtggatttgc tcatcagcgc agattcaaag aaatcaaagg ctcaagtgct
     9121 cgacaggttg gcgcgcggag aaacagatat tgtgattgga acgcacagcc ttctggaacc
     9181 agaagttgtg tttgagtccc tgggcctggt catcatagac gaacagcatc gcttcggtgt
     9241 agaacagaga gaatctctac gtcgcaaggg aaagaatcca gacgtcattg gaatgaccgc
     9301 aacaccaatc cctcgaactc tgtgtctgac agaatttgcg gatctcagcc tggttacgct
     9361 gaaggaaaaa cctgcgggca gagtgccgat caagaccatt cggctggcag aagatagacg
     9421 tcctggcctt tacaaatcca ttcgaaatca cgtttcaaac ggcagacagt gttacatcgt
     9481 ttatccggtg attgaagaaa gcgagaaact ggatgtgcgt gctgcaacag acgcgtatga
     9541 agaacttcgc acgagcattt ttcctgacct gaaagtggag ctgcttcacg gcaaaatgaa
     9601 aacagaagat aaggatagag tctttcgcga gtttcgcgcc ggtagagttc agattcttgt
     9661 ctccactact gtcattgaag ttggtgtgga cgttgccaac gcgaccatca tggtcataga
     9721 acacgcagat cgttttggaa tttcacaact acatcaactc aggggccgcg ttggaagagg
     9781 gagcgaagaa agcttctgca ttttaatggc cggtgaaaca acagaggacg gcgaagagag
     9841 gctcaacgcg atcgtaaact ctgacaatgg ctttgaactt gcggaagtgg acctcaagat
     9901 tcgcggcccg ggagaaattt tgggaaaacg acagcatggc gtagatggcc tgagaattgc
     9961 gagtctgctt gaagatagaa aactagtaga ggagtccttc gcagccgcaa acgagtggac
    10021 agatctaccc ccgggcgccc ttgagtttat caaaaatcgg ttttcatctg cggagcaaag
    10081 tctcagctga tcttcatgca gacgatcccc gctccatcca gagatatcgt cagtttcttt
    10141 agagcccttg caaacatagg cgtgaagcag accatgagtc cgccggaaaa gaagcgtgta
    10201 gtgctcagca atcagctctt cctgatcgcc gcgggcgtca ctttgtcttt tctacctctg
    10261 ggactggccc tgggcacatc tgccattggc actgtgacga gcttgatcga cactttgctt
    10321 ctggccgggt gtttctttct caactatcgc ggccagtata aaatggctcg agtgatgggc
    10381 ctcgttgtcg gaaacagtct cacgttcttc tgggttctcg ctctgggcaa tcagatgtat
    10441 ccggaacttt tttatttcac cctggcagcg gcacccctgt tgttcattgg cccggatcag
    10501 aaaaaatctc tctacgttct ctctttgctc tctgttgtgc tcttttttgt ttcgcatgaa
    10561 ctcaaagcga agattgcacc catgatcctg attcctggag atatcgcact tctggctggc
    10621 tatagcaccg gtgttctttc tttcattaca ctgttaagta ttctttacta ttttcaccgt
    10681 ggcacagaac gagtggaaag cgagctggat tcggaaagaa agaagtcaga tcgtttgctt
    10741 ttgaatattc ttccagaacc aatcgcggat cggctgaagg caggagaaag actcattgca
    10801 gatcgatttg atcgagccac agtgctcttt gcagacattg taaatttcac accaatgtcc
    10861 ggaagcatgc ccgcggatga agtagtgagt attctcagca gaatcttctc cgcattcgac
    10921 atgctttgcg aaaaaaatgg aatggagaaa atcaaaacaa tcggtgattc ttacatggcg
    10981 gctgcgggct tacccgagcc agatgaaaac catatgcaat ccgcagcccg catggcactg
    11041 gatatgcaac aaatgatcct ggagatggcc cgcagagaag gaatggatct aagcattaga
    11101 atcggaattc attccggccc ggtagtcgcc ggggtcattg gagaaaagaa attcatctac
    11161 gatctgtggg gtgacaccgt taacctcgca agcaggatgg aatcccacgg aattcccggg
    11221 caaattcagg tttcccagca catatacgaa actctgcact ctaccttcac atttgaaacc
    11281 agaggcctca tcgaggtgaa ggggaaaggg cccatgcaaa cgtatttttt aaccggttcc
    11341 acccgcgatt gagcaaaatt cgggtgactg atctgaaaac ctcgtctaat ctgagaggat
    11401 tttattttgg tttcggaatt tgccaccaga gttgccctcc ttgtgctctt cctatccttg
    11461 ctccttggca cggcattcgc actctatttc caggacagac gcaacactct caatagacta
    11521 ctcgcactgc aactcatcct gattgcgatc tggcaataca ccggttttct tcacgcactc
    11581 ctttccgcag gcccgcctct ggtgagacct cttcaaattg tagccggtgt tgtagcagcg
    11641 tatgtgtttt ttgttctggg gaggcacctg ggcagactcc gagccggcaa atgggaagca
    11701 gcaggcgctg cctggatatt cttaatattt atatattata tggttcgtat actaattcct
    11761 gattctggaa atgcagcgga gtttgtactg gtcgacggta ggccagtgta tggaccaact
    11821 ccggcctatg tcacttacac gagcacattg tccgcatttt acggagtagg aatcttccat
    11881 attctaaaag caaggagacg cgaagaagat acggttatgc gaaagagaat gctcttgatt
    11941 gtactaacaa tcggggccgc tgccatgtcc ggcatcatct ttattaatac gttctacata
    12001 ctgaatatcc agatgccctt ccccatcgaa agagtgatca tggtaagtgc gcttctattc
    12061 ctgggttatt ccgttctcgg agaaagagca tttagcaatg aaaacctgat tcgcctggtc
    12121 agagaaaagg aaaaggcgat tcgagataga aatcgtgtga tcgaaagtga actcgatctt
    12181 gcgcgcatga tccacagtcg tctttttccg gacaagccac cgaggattcc tggatatgac
    12241 attcagggcg cctgcatttc cacggataaa gtgggcgggg atttttacga tttctatact
    12301 cgcctggaaa atctgggaat cttcattgcg gacgtctccg gtcatggaat ccccgccgcg
    12361 ttcattgcct cctgcacgaa aatggctttc aactattctg ccgggcatgc ggaaaacggg
    12421 acgcaacttc tcaaaaagat ggatgtggcc atcgcacgcc gcgctgtaca atcgatgtac
    12481 gttacagctg tttacgccga aatcgactat gcaagtcgcc agctttccta ttgcacggcc
    12541 ggacatggac caatactgct tcatcgcaga tccgacaata cactccaaca tttgaaggca
    12601 aaaggatcgc ctctcgggct tatgaccggc aaaccatttg aaatgcgaac cctccagctt
    12661 attccaggag atcgccttgt attttttacg gatggcctga cagaatctat caaccacgag
    12721 cgcaaaccat atacggagga aa
//
LOCUS       DAOQJL010000037        12598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2462_length_12598_cov_4.257116, whole genome shotgun sequence.
ACCESSION   DAOQJL010000037 DAOQJL010000000
VERSION     DAOQJL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2462_length_12598_cov_4.257116"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLG78_02835"
     CDS             <1..233
                     /locus_tag="PLG78_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22072.1"
                     /translation="GYYLASYAGKLAGEYARAADFGECVRLREPHNVRNLVNLADIYR
                     ASGDKARAGKILEEALLLDAGNENAMKIKSLL"
     gene            complement(230..1300)
                     /locus_tag="PLG78_02840"
     CDS             complement(230..1300)
                     /locus_tag="PLG78_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HNE22073.1"
                     /translation="MKIEVRNLSKKFGQYQALSNVNIEVPSGELVSLLGPSGCGKTTL
                     LRIVAGLEFPDSGQILFSGSDASAQTVRERHVGFVFQHYALFRHMRVFDNIAFGLRVK
                     PRSIRPDKQLITKKVNELLKLVQLDHMADRFPDQLSGGQRQRVALARALAVEPNVLLL
                     DEPFGALDAGVRRELRRWLRRLHDEIQVTSLFVTHDQDEALEVSDRVIVMNQGRVEQE
                     GTPSEVYSKPASPFVLNFLGNVNLFRGRIEQGKVKIGQMDLEAPGGGQGSENRDALVY
                     VRPHELDLHRSAPADPIFQGLVNYMNLAGSMARIEVRRDDGQIVEVEMPRDRLVAEGF
                     AVGDAVFVSASRTSIFVDDYSI"
     gene            complement(1297..2136)
                     /gene="cysW"
                     /locus_tag="PLG78_02845"
     CDS             complement(1297..2136)
                     /gene="cysW"
                     /locus_tag="PLG78_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNE22074.1"
                     /translation="MRKTRSTQDSRTVQFTLIFLAVLFFALFLAVPLISVFIGAFGSG
                     LSVWADAILDPAALAAIKLTLIVAGISVPLNTLFGLVVAWTMKFEFTGKSLLLALIDL
                     PFSVSPVISGLIYVLIFGLQGWFGAWLQDHDIKIIFALPGLVLATLFVTFPFVARELV
                     SLVEAQGNEEELAALSLGASGFQIFWKVTLPNLKWALLYGVIQCNARAMGEFGAVSVV
                     SGHIRGMTNTVPLHVEILYNEYNFTASFAVASILAFLGVITLIAKTFLEKRTQQERRK
                     ASA"
     gene            complement(2133..2954)
                     /gene="cysT"
                     /locus_tag="PLG78_02850"
     CDS             complement(2133..2954)
                     /gene="cysT"
                     /locus_tag="PLG78_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNE22075.1"
                     /translation="MRKKQKVLPGFGLSLGITIFYLGIIVLLPLAALGMKAFSGGASP
                     FLDAAFDPRSIASYKISFGASLAAALVNALFGLLLAYVLVRYEFPGRAILDALVDMPF
                     ALPTAVSGIALTAVYAPTGVFGSAFARIGIKTAYSPVGIFIALVFIGLPFVVRTIQPV
                     LEEVDHEMEEAAASLGASRFQVFVKIIFPEILPALLIGTSLAFARAVGEYGSVVFIAG
                     NMPFQTEITPLLIITKLEQYDYAGAATLGAVMLTASLLILLIVSALERLTGRHEA"
     gene            3109..4845
                     /locus_tag="PLG78_02855"
     CDS             3109..4845
                     /locus_tag="PLG78_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22076.1"
                     /translation="MSALTIGDNRMKDSTIGRRALLIALAAGFAVTGNLFAYETKYTD
                     KKTDEKTENNTEFKVDGVIFAGYERTDKENSGVPDANGTNARNQGFDIKRAYLNLIGN
                     VTDGDYKGYGFRVTFDGGQVLGGTAATTSASGNNIHVPELKFAYMTMPLFSAGDFGSS
                     SLRLGMQHTPTTDNQTGASMEGYWKHRYLDTLPTENVNMSSSADTGIGYIHKADYFGL
                     HLLLANGEGFRKLNAQNIQAPTTFSSIATNAAAVRTNLRSMSTTGATADSYGLDLYGA
                     LSVKPTGKSKDFEVAINFPFRLQNVTGIRDEEAKTTAVDISTLSSPKLQAYVGDKRVK
                     QDQSVGSELDVSADVGGLKITLGTGGIRYKDKRASTLSYDSTGLTGLLTNIDYTRYYN
                     IDKDAYGSSNYGYLHVRYKWVGAMFRDIYGTGSSGSLSALPSKSYLQQLAEADIADGQ
                     IGNGNVTMFSTHGTSPTIDLGKARFRKQLMAIEFFPTPRWSIAVGMSRQTSTDPDGYK
                     TKVSGLAQIPTIGTAAGNTSVPPGSSTNAESQVNTLIVSQNNNQATLQATDIAGEVKE
                     NKQFFVRAAYEF"
     gene            5190..6185
                     /locus_tag="PLG78_02860"
     CDS             5190..6185
                     /locus_tag="PLG78_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE22077.1"
                     /translation="MKLTIPKIVLGLAALCSSPAFAQDVTLLNVSYDPTRELYKEFNA
                     AFAKYWKGKTGQTVAINQSHGGSGKQARAVIDGLEADIVTLALAYDIDEIATRSRLIA
                     PGWQKELPNNSTPYTSTIVFVVRQGNPKNIRDWDDLVKPGVSVITPNPKTSGGARWNY
                     LAARGYALKKYGSEEKAREFLKQLFANVPVLDSGARGSTTTFAKNGQGDVFISWENEA
                     YLIQNEFGAGKFQIINPSISILAEPPVTVVDKTAERRGTKKIAEEYLKYLYSDEGQRI
                     AGKHHYRPSNPAIAGQFPFPQIKLFTIAELFGGWASAQKTFFADGGEFDQIYKRK"
     gene            6189..7916
                     /locus_tag="PLG78_02865"
     CDS             6189..7916
                     /locus_tag="PLG78_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22078.1"
                     /translation="MKTQIRATLRKTTVALSLLGGSQAIMAYETSWFDKKVDEKEAIS
                     SFKADGTIFAGYERADYEKNGVLEPGGTRPATSTGFIINRAYLNLRGDVNEGPWKGAN
                     FRVTVDAGVLGNNVLGTTNSTTSTGNSAQVAELKFAYFSLPLYTTSFGDGSVRIGQQN
                     VAIVDGQAGYSNESYWKHRYIDQNAVEKSGFAGGSADRGISFIHKADYFGLHLMLSNG
                     EAFRRNNAQFVFNPTGSTGAVNVATTGVLTAAQQTQVRTSLTNLVNGNNGTTNDSYGY
                     DLYGIPSFHPTGKAKDIDISIGLPFRLQNTTGIKRQEYEFLTADISTINSPRFDYYKG
                     DARAKQDTAFGQELAAAFDFGDLKFTIGAGHALLKDKRSSAFRIDQNGLNTLVATQGS
                     KPFVDYTTFYQADQDAYGQTNWGYIHARFLSFGFIARQIYGTGSGTISALPKKSYLQQ
                     LAEVDIRDGNIGNGDVTLAASRGSSPSIDLGKARFKRETYALTWNPFARLSVALGVEK
                     LKSKDKNGETTKVNNLDNIATFSTTTTTLSSQVNSYLATQGAPLVANDFGGKEKENKQ
                     VFIRAQYEF"
     gene            complement(7913..8092)
                     /locus_tag="PLG78_02870"
     CDS             complement(7913..8092)
                     /locus_tag="PLG78_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22079.1"
                     /translation="MMAPAIAIHKGKSWVKILEALKSSTLGLPRMKATKKKPARGGAG
                     GTIGELAFGEILWFL"
     gene            8070..8939
                     /locus_tag="PLG78_02875"
     CDS             8070..8939
                     /locus_tag="PLG78_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNE22080.1"
                     /translation="MAIAGAITFYAPWIFTWFNAPMITWGLSFIMLGMGITLTFEDFR
                     RVSRIPLQVVLGTFLHFTVMPALGWTGAKLFSLSDPFAVGLILVACCPCGTASNVINY
                     LAKSDVPLAVTITAISTVAAVAMTPILTGMLAGSRMTVDALGLLGSTLQVILLPVTLG
                     ILLKRLLPRAAEAILPFSPPAAVIIIVMIVGSVLGQNRTQIIEAGPSLLGAVVFLHAG
                     GFLIGFLLARIFLDRIASRTISVEVGMQNSGLAVVLARANFANPLVAVPGAISSFVHS
                     AIGSVLAVYWRKH"
     gene            8936..9718
                     /locus_tag="PLG78_02880"
     CDS             8936..9718
                     /locus_tag="PLG78_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE22081.1"
                     /translation="MKVSITHIDTACFLLEIGGLRILTDPVFDPPGKIYSFGFGTFSK
                     KYSAPALSPKEIGRIDLVLLSHDQHEDNLDKSGREFIKKIPLVLSTRPAAKRIPGITG
                     LDEWQSFEIQGTSVKVTATPAQHTGLRMLNPIAGKVIGFILEWPGQTNGVTYISGDTV
                     YFQGINRIAERYAKIDTAILHTGRAGFPYLTGALHYTFHAKDALKAAEVLAPRRIIPV
                     HCAGWWHFREKEEHAHEVYSKSKFADRIVQLSPGKPLVLGPE"
     gene            9744..12131
                     /locus_tag="PLG78_02885"
     CDS             9744..12131
                     /locus_tag="PLG78_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE22082.1"
                     /translation="MDQVVKSAMRAIHFAVVTILLCCQCTWIHDRAVRSRDLTGPSLP
                     DIVDADPRAMYHPGRIRPTAVQNVQQTKTGQLVARNEFVVRLNPAQSPARFLKDLERA
                     KIQANIIGGVPTFHLVQIEIQGDVNQETTEAKLRTLPLVEDVFLHFIRTAQASPQDWH
                     IRNYGLDSVWKEFSGAGVRIAVLDTGIDDSLSTFKGRIVEPYSVLTTSARFEDGQIKK
                     KGDVLRVVDHGTRVASVAAGSGDEKGILPGVAPRAEIMPIQVMGFHVIDESIYTNDLT
                     ILEGLARAMALRSHIINLSVGTDYAQILRDVHDPAARETILTAIKKESRSTQSLYNQA
                     FARAREMNIWIVAAAGNAGDSAELEPIASHPLVISVGALDSDDRRASFSNYGTAVTTY
                     APGVGVPTYIPGGMLSASSGTSFSAPYVAGVLALLRSSSPNLTFDQARASVVRSNIIA
                     RTTILPEDNSTPVFDPVGLLSHGARTDQRGRFRTFATRFGDLFIATNDAPGVKLGKIL
                     DYYNRIEVHSELDPESRAAGPLVATNFGVIRERVRRGAKYGEMSVVAEAPLLEDQLTF
                     LRENISTSDFIAIVCSRKMDRKCIPYLLQRLDRKQWNANTLVALWTFKEPGTHEKIEA
                     YLALIQSNQEWVDDECFAIRGLVETTSEPDAKDQKLIDQSILRYRAFEGKFGESGLVD
                     YRADDLAFALIKAGRSEGLFLCIDTIEKIENRLETLANWGVAVSSTIPAGFGNTPEEL
                     GYRLRSLQNMLNESTTFPEKYNHQRPVEERMKVRDKFRDYAKKAEFSRARFRLAQ"
     gene            complement(12143..>12598)
                     /locus_tag="PLG78_02890"
     CDS             complement(12143..>12598)
                     /locus_tag="PLG78_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE22083.1"
                     /translation="YSYGLVGWNSRISAFQAAYIRLSIPHVKSRIESRRAAVKQYRTH
                     LGELPSVRPPEGFEENGYLYCILYNPSERSSVEARLKEKGIGFANTYPGSMSSQAGAK
                     GYIKAAVGGDNARKLAESILNPPLFPYMTDAEVSEVVQSLRPKAAPFVG"
BASE COUNT         3430 a         3296 c         3069 g         2803 t
ORIGIN      
        1 atgggtacta cctggcttcc tacgccggga aactggctgg cgaatatgcc agagctgccg
       61 actttggcga atgcgttcgc ctgagagagc cgcataacgt tcgaaacctg gtaaacctgg
      121 cggacatcta tcgcgcatcc ggagacaaag caagggccgg gaaaatcctg gaagaagctt
      181 tgctcctgga cgcagggaat gagaacgcga tgaagatcaa atcgcttctt tagatagaat
      241 agtcgtccac aaatatcgag gtccggcttg cgcttacaaa cacggcgtcg cccacagcaa
      301 agccctcagc tacaagtctg tctcttggca tttcgacttc cacgatctgc ccgtcgtcgc
      361 ggcggacttc aatgcgagcc atggacccgg ccagattcat gtaatttact agcccctgga
      421 agattggatc cgcgggcgcg cttctatgca aatcaagttc atgaggacga acatagacta
      481 gagcgtctct attctctgag ccctgaccgc ctcccggtgc ttcaagatcc atctgaccga
      541 tctttacttt cccttgttcg attcggccgc gaaagaggtt tacattaccg aggaaattca
      601 aaacaaacgg gctggccggt ttggaataaa cttccgaagg cgttccttct tgttctaccc
      661 gtccctgatt catcactatg acgcggtcag aaacttcgag agcttcgtcc tgatcgtgtg
      721 tgacaaagag actcgtgacc tgaatctcat cgtgaagacg acgaagccat cgtctcaatt
      781 cacgacggac cccggcgtcg agtgcgccaa agggttcatc cagaagtagc acatttggct
      841 caacagccag tgcacgcgcc agagcaacac gttgacgctg cccaccggac aattgatctg
      901 gaaatcgatc cgccatgtga tccagctgga ctagcttcaa aagctcattc actttcttcg
      961 tgatgagttg tttgtccggc ctgatgctcc tgggctttac gcgaagtccg aatgcgatat
     1021 tgtcaaaaac gcgcatatgg cgaaacaatg cgtaatgctg aaacacaaac ccaacgtgcc
     1081 gctctcgcac cgtttgtgct gaagcgtccg aaccactgaa cagaatctga cccgagtctg
     1141 gaaactcaag gcccgccaca attcgcaaga gagttgtttt gccgcaaccg gacgggccaa
     1201 gtaaggatac aagttcgccc gacgggacct ctatattgac attggagaga gcctgatact
     1261 gaccaaattt ttttgaaaga ttgcgaactt ctatcttcat gccgatgcct tccttctttc
     1321 ctgctgcgtt cgcttttcaa gaaacgtttt tgcaatcaga gttatgacgc cgagaaatgc
     1381 gagaatggat gcaaccgcaa atgacgccgt aaagttatat tcattgtaaa gaatttcaac
     1441 gtgaagagga acggtgttgg tcatcccgcg aatgtgaccg gagacaacgg aaacagctcc
     1501 gaattcgccc atggcgcgcg cattgcactg aatgacaccg tacaacaatg cccactttag
     1561 atttggaaga gtcactttcc aaaaaatttg aaacccactg gcacccagcg aaagagcagc
     1621 cagttcttcc tcattgccct gagcttccac aagcgatacg agctctcggg caacaaatgg
     1681 aaaagttaca aacaaagtcg caagaacaag tccaggaagc gcgaaaataa tcttaatatc
     1741 atgatcctgg agccacgctc caaaccagcc ctgcaatcca aaaatcaaaa catagatcaa
     1801 tccggagatg acaggcgaaa ccgaaaacgg caaatcgatc agagccagca aaagtgattt
     1861 tcctgtgaac tcaaatttca tagtccaggc aaccacaagg ccgaataacg tattgagcgg
     1921 aacggaaatg ccggcaacga tcaacgttag tttgattgct gcgagtgcgg ctggatctag
     1981 aattgcgtcc gcccaaacgg acaatccaga accaaaggcc ccgataaaaa cggaaatcag
     2041 gggaacggcc agaaagagcg cgaaaaacaa cactgcaaga aagatgagcg taaactggac
     2101 tgttcgcgaa tcttgagtgg agcgggtttt cctcatgcct cgtgcctccc cgtcagtcgc
     2161 tctagagcgc ttacgatcaa aaggatcaat agcgacgcag tcagcatcac agcgccgagc
     2221 gtggctgctc cggcgtaatc atattgttcc agtttagtaa tgatcagaag tggtgtgatt
     2281 tctgtttgaa aaggcatatt gcccgcgata aaaacaacag agccatactc tcctacggca
     2341 cgcgcgaaag ccagagacgt tccaatcaag agagccggca gaatctcagg aaaaataatc
     2401 tttacaaaga cctggaagcg actcgcgccc agacttgcgg ctgcctcttc catctcatgg
     2461 tccacttctt cgagcaccgg ttgaatggtg cgaaccacaa atggaagacc aatgaatacc
     2521 agagcaatga atatgcccac gggcgagtag gccgtcttga ttccaatgcg cgcgaaggca
     2581 ctgccgaaaa cacccgtcgg tgcataaacc gcagtcaatg caattccaga gactgcagtg
     2641 gggagagcaa agggcatgtc gaccagagca tctagaatcg cgcggccagg aaactcgtaa
     2701 cgcaccagaa cgtaggccag aagcagtccg aacaatgcat tcacaagcgc ggctgccaga
     2761 gacgcgccaa agctgatttt gtaggacgca atggaccttg gatcgaaagc agcatccaga
     2821 aacggggatg cgccacctga gaacgctttc attccgaggg ccgccagagg caaaaggacg
     2881 ataattccga ggtaaaagat agtgattccc agagagagcc caaaacccgg caaaaccttc
     2941 tgcttcttcc tcatggaaca aaaaaaatcg agtccaccgc cgggtcaatc taataacgac
     3001 tcacttggta ggatttcaag ggaggtccga ttgaaaccgt caaatccttg cctcgttgtc
     3061 ctcggattgt aacaaagtcc agacattact ggagatcaac cggcacagat gtcggcgcta
     3121 acaatcggag ataatagaat gaaggacagc accattggac gaagggccct gctaatcgcc
     3181 cttgccgcag gatttgcggt cactggaaac ctttttgcgt acgaaactaa gtacactgac
     3241 aaaaagacag atgaaaaaac tgagaataac acagaattca aagtagacgg tgtgatattc
     3301 gccggatacg aaaggacaga caaagaaaac agcggcgtac cggacgcaaa cggaaccaat
     3361 gctcgcaacc agggctttga catcaaacgc gcatatctca acctgattgg gaacgtcacc
     3421 gacggcgact acaagggcta cggattccgc gtaacgtttg atggcggtca ggtgttaggc
     3481 ggaacagcgg ccactacgtc cgcttcagga aacaacatcc acgttccaga actgaagttt
     3541 gcctacatga ccatgcctct cttttcagcg ggagattttg gatcgagctc tcttcgcctc
     3601 ggcatgcagc atactcccac caccgacaac cagactggcg cgtccatgga aggatactgg
     3661 aagcatcgct acctggacac tctgcctacg gaaaacgtaa acatgtcttc ttcagcggat
     3721 acagggattg gatacattca caaggcggac tactttggac tccatctgct ccttgcaaac
     3781 ggagaaggct ttcgcaaact gaatgctcaa aatatccagg cccctaccac gttttcaagc
     3841 atcgcaacga acgcggccgc tgttcgcacg aacctgcgtt ctatgtccac aacaggtgcg
     3901 actgcagaca gctacggcct cgatctttac ggagccctct ctgtaaagcc aaccggaaaa
     3961 tcaaaagatt ttgaagttgc catcaacttt ccgtttcgcc tacagaacgt tacaggcatt
     4021 cgagatgaag aagcaaaaac aactgccgtt gacatcagca cgctatcgag cccgaagctt
     4081 caggcatatg ttggcgacaa aagagtgaag caggatcaat ctgttggttc agaactcgac
     4141 gtatccgcag acgtgggtgg tctcaagatc actcttggaa caggcggcat cagatacaaa
     4201 gacaagagag caagcactct gtcctatgat tccaccggcc tcaccggttt gctcacaaac
     4261 atcgactaca ctcgctacta caacattgac aaagatgctt atggatcgtc taactacggt
     4321 tatttgcatg tgcgttacaa atgggtcggc gccatgttcc gcgatatcta cggaactggt
     4381 tccagtggat ctctgagtgc acttccaagc aaaagctatc tccagcagct agcggaagca
     4441 gacattgctg acggacaaat tggaaatgga aacgtaacca tgttctctac gcatggaacg
     4501 agcccgacca tcgaccttgg taaggcaaga ttccgtaaac agctgatggc gattgaattc
     4561 tttcccactc cacgctggtc catcgcagtt ggcatgagca gacagacttc cacagatccg
     4621 gatgggtaca aaacaaaggt gagtggcctc gcacaaattc caacgattgg aacggcagca
     4681 ggaaacactt ctgttcctcc gggttcttca accaatgcgg aaagccaggt gaacactctg
     4741 attgtatcgc agaacaacaa tcaggctaca cttcaggcaa cagacatcgc aggcgaagtc
     4801 aaagaaaaca aacaattctt cgtccgcgct gcttacgaat tctaaaattc gcaagcgttc
     4861 ctgcttcaaa acccgccttc ttaaggcggg ttttttgttt tgtcggcaga gaacacccaa
     4921 ttccccgcca tgcccggatc tctagaatcc aaccccaaca acaacacttc tacctttcac
     4981 ggcgggcctc cccttgcctt aagacgataa aaatagtcgt atattttaat tgacgagccg
     5041 gaagcctttg ctttctatgg ctcaattagc gattgaaata gtcgtatata aaatccagac
     5101 tccgacattc tcttcatgaa agaaggaacc cgaaaggagc ccgggcttta atgacgactg
     5161 atttgatcgc atatttcagg agtatcccta tgaaactgac cattccgaaa atcgtcctgg
     5221 ggctcgcagc actctgttca tccccggctt tcgcgcagga cgtgacacta ctcaatgtct
     5281 cgtacgatcc aacacgcgaa ctctataagg aattcaacgc agcgtttgca aaatactgga
     5341 aaggcaagac aggccagaca gttgcgatca atcaatcaca cggtggatct ggcaagcagg
     5401 cgcgcgcagt catcgacgga ctggaagcgg atatagtcac tcttgcactg gcgtatgaca
     5461 ttgacgaaat cgcaacgcga tcgcgcctga ttgcgcctgg atggcagaag gaacttccaa
     5521 acaacagcac accttacacc tcgaccattg tctttgtagt aagacaggga aatccaaaaa
     5581 atattcgcga ctgggacgat ctggtgaagc caggagtttc cgtaatcacg cctaacccaa
     5641 aaacatccgg cggcgcaaga tggaattacc tggcagcacg cggttacgct ctgaagaaat
     5701 acggaagcga agagaaggcg cgagaatttc tcaaacaact tttcgcaaac gtgcctgtgc
     5761 tggattccgg agccagagga tctacaacaa cattcgcaaa gaacggccag ggagatgttt
     5821 tcatttcctg ggaaaacgaa gcgtatttga tccagaacga atttggagcc ggtaaattcc
     5881 agatcatcaa tccatccatc agcattctcg cagaaccgcc agttacggtt gtagacaaga
     5941 cagcagaaag acgcggaaca aaaaagatcg ctgaagaata cttgaagtat ctgtactcag
     6001 acgaagggca gcgcatcgca ggcaaacatc actatcgccc gagcaaccct gctatcgcgg
     6061 gccagttccc tttcccgcaa atcaaactct tcaccattgc agagctcttt ggtggctggg
     6121 ccagtgctca gaagactttc ttcgcagacg gcggagaatt tgaccagatc tacaaaagga
     6181 aataagacat gaaaacacag atacgagcaa cactacgcaa aactacagtc gcgctctccc
     6241 ttctgggagg cagccaggca atcatggcct atgagacaag ctggtttgac aagaaagtag
     6301 acgagaaaga agcaatcagt tctttcaaag cagacggtac gatctttgcc ggatatgaaa
     6361 gagccgacta tgaaaagaat ggcgttctgg aaccaggcgg tacgagaccc gcaacgtcta
     6421 cagggtttat catcaaccgt gcatacttga atctgcgcgg tgatgtaaat gaaggtccgt
     6481 ggaaaggcgc aaactttcgc gtaaccgtgg acgcaggcgt tcttggaaac aacgtcctgg
     6541 gaactacgaa ctcgaccact tctacaggta acagcgcgca ggtggcagaa ttgaaattcg
     6601 catacttttc gctgcccttg tataccacat ctttcggaga cggtagtgtc cgcattggcc
     6661 aacaaaacgt tgcgattgta gacggtcagg cgggctattc caacgaaagc tattggaaac
     6721 acagatacat cgaccagaat gcagtggaaa aatctggatt tgctggcgga tctgcggata
     6781 gaggcatttc cttcattcac aaagcggatt actttggtct gcatttgatg ctttcgaacg
     6841 gagaagcgtt tcgcagaaac aatgctcagt tcgtcttcaa ccccactggc agcacgggtg
     6901 ccgtcaacgt tgccacaaca ggtgtattga ctgcagcgca gcaaactcag gtgagaacga
     6961 gccttacaaa cctggtgaat ggaaataacg gaaccacgaa tgactcgtac ggatatgatc
     7021 tctatgggat tccgagcttt catccaacag gcaaagcgaa agatatcgac atatcgatag
     7081 gccttccatt tcgcctgcaa aacacaacgg ggatcaaacg gcaggaatat gaattcctga
     7141 ctgcagatat ttctaccatc aactccccgc gttttgacta ttacaaaggg gatgccagag
     7201 caaagcagga cactgcattc ggtcaggaac ttgctgcagc cttcgacttt ggcgatctaa
     7261 aattcacgat cggcgcggga cacgctctcc tcaaagacaa gcgatcctct gcttttcgca
     7321 tagatcaaaa cggcctgaac actctcgtgg caacacaggg ctcaaaaccg ttcgttgact
     7381 acaccacgtt ctaccaggca gatcaggatg cgtacggtca aaccaactgg ggctacatcc
     7441 acgcgcgctt cttgtcgttt ggattcattg cccgccaaat ttacggtact ggttctggaa
     7501 ccatatccgc ccttccgaag aaatcctacc tgcagcaact tgcagaagtg gacattcgcg
     7561 acggaaatat tggcaacggc gacgtgactc tggcggcctc tcgcggatcc tcaccttcga
     7621 tcgatctagg aaaagcacgt ttcaaacgcg agacgtacgc actgacgtgg aatcccttcg
     7681 cacgcctttc tgttgctctg ggtgtggaga aactcaaatc aaaagacaaa aatggagaaa
     7741 ccacgaaggt aaacaacctg gacaacatcg caacgttcag cacaacgacg accactctct
     7801 caagccaggt aaacagctac cttgcaactc agggtgcgcc tctcgttgcg aatgactttg
     7861 gcgggaagga aaaagagaac aaacaagtct tcattcgcgc gcaatacgaa ttctaaagaa
     7921 accatagaat ttctccgaaa gcaagttcgc cgattgttcc gcccgcccct ccccgggcgg
     7981 gctttttttt cgttgctttc attcgcggca gtcctagcgt cgaagacttg agggcttcga
     8041 gtatcttcac ccagcttttt cctttgtgga tggcaatcgc tggcgccatc acgttttacg
     8101 cgccctggat tttcacctgg ttcaacgctc caatgattac atggggcctg tctttcatca
     8161 tgctcggaat gggaatcact cttacatttg aggactttcg ccgcgtatcg cgcatccctt
     8221 tgcaagtagt ccttggcacc ttccttcatt tcactgtgat gccagcgctt ggatggacag
     8281 gggcaaaatt gttttctctg agtgatccat ttgcagttgg cttgattctg gttgcctgtt
     8341 gtccgtgtgg aacggcgtct aacgtcatca actacctggc aaaatcggat gttcctctgg
     8401 ctgtgaccat cactgcgatc tcaactgtgg ctgcagttgc catgactcca atcctgacag
     8461 ggatgcttgc aggaagcaga atgactgtgg atgccctggg cctgctcgga tctacgctcc
     8521 aggtgattct actgccggtc actctgggta tcttactcaa aaggctcctt cctcgcgcag
     8581 cagaggcgat tcttcctttc tccccgcctg cggctgtgat catcattgtg atgatcgtag
     8641 gttcggtcct gggtcagaat cgcacgcaga tcatcgaagc aggaccctct cttcttggtg
     8701 cggttgtatt ccttcatgcg ggtggtttcc tgatcgggtt tctacttgcg cggatattcc
     8761 tcgatcgcat agcatcgcgg accatttcag tggaagttgg aatgcaaaac tcgggtcttg
     8821 cagtggttct cgcacgcgcg aatttcgcaa atcccctggt ggctgttccg ggtgcgattt
     8881 cttcttttgt gcattcagca attggaagtg tactggccgt ttactggagg aaacattgaa
     8941 agtctccatc acacacatcg acacagcatg ctttctgctt gaaatcggcg gcctgcgcat
     9001 actcacagat ccagtctttg atccaccggg aaaaatctac agttttggtt tcggtacttt
     9061 ctcaaagaag tattccgcgc cggcactcag cccgaaggaa attggacgaa ttgatcttgt
     9121 tctactctcc cacgaccaac acgaagacaa cctggataaa agcggaagag aattcataaa
     9181 aaagataccg ctcgttctat ccaccaggcc tgcggcgaag cgaatccccg ggatcactgg
     9241 acttgatgaa tggcagagtt tcgaaataca aggaaccagt gtaaaggtta cagcaacgcc
     9301 ggcgcagcat acaggtcttc gcatgctaaa tccaattgca ggcaaggtga ttggattcat
     9361 actagaatgg cccggccaga ccaacggggt gacctacatt tccggagata ctgtttactt
     9421 ccagggaatt aatcgcatag cagagcgtta tgcgaaaata gacactgcca tcctccacac
     9481 tggaagggcc ggatttccat atctgacagg agcgctccac tatacttttc atgcgaagga
     9541 cgcgctcaaa gcagctgaag tgcttgctcc acgcagaatc atcccggtgc attgtgcagg
     9601 gtggtggcat ttcagggaaa aagaagaaca cgcgcatgaa gtgtactcaa agagcaagtt
     9661 tgcagacagg atcgttcaac tttctccagg gaaacccctg gttctcggtc cagaataata
     9721 ctgagttttt ctcttaccgg gaaatggatc aggtcgtcaa atcagctatg cgtgccatcc
     9781 actttgcggt tgtgaccatt ctcctctgct gccagtgcac atggatccac gacagggccg
     9841 tgcgctccag agatctgaca ggaccatctc ttcctgacat tgtagatgca gatccgcgtg
     9901 ccatgtatca tccgggtcgg atcagaccaa cagctgtaca gaacgttcaa cagactaaaa
     9961 caggtcagct ggtcgcacga aacgaattcg tagtccggtt gaatcctgcg cagtctccag
    10021 cccgcttcct caaagacctg gagcgcgcga agattcaagc aaacatcatt ggaggagttc
    10081 ctacattcca cctggttcaa attgaaatcc agggcgacgt caatcaagaa acgacggaag
    10141 cgaaactccg cacgttgccg ctggtggagg acgtattttt gcacttcatt cgcacagcac
    10201 aggcctcacc gcaagactgg catattagaa actacggact ggactccgta tggaaagagt
    10261 tctcaggcgc gggagtgcgc attgcagttc tggatacagg catagacgat tcgctgtcga
    10321 ctttcaaagg tcgcatcgtc gaaccgtatt ctgtactcac aacatccgct cgctttgagg
    10381 acggccagat caagaaaaag ggagatgtac tgcgcgtcgt ggatcatgga acgcgcgtgg
    10441 caagtgtggc agcggggagc ggagacgaaa agggcattct acctggcgtt gcaccccggg
    10501 cagaaattat gcccattcaa gtcatgggat tccatgtcat tgacgaaagc atctacacaa
    10561 acgacctcac cattctagaa ggcctggcga gagcgatggc gcttcgctct catatcatca
    10621 acctcagtgt tggcactgac tatgcacaaa tactgcgcga tgttcacgat ccagcggcac
    10681 gcgaaaccat cctgaccgcg ataaagaaag aaagtcgttc cacacaatct ctgtacaatc
    10741 aggcattcgc acgcgcgcgc gaaatgaata tctggattgt cgcggcagcc ggcaacgccg
    10801 gagattctgc agagcttgaa ccaattgctt cgcatcctct tgtgatttca gtgggagctc
    10861 tcgattcaga tgatcgtcgc gcttcttttt ccaattacgg taccgcagtc acaacatatg
    10921 ctcctggcgt cggagttccc acatatattc ctggaggcat gctgagtgca tcatcaggaa
    10981 cgtccttcag cgctccatat gtggccggtg ttctcgcact attgcgatcg agcagcccga
    11041 acctgacttt tgatcaagct cgtgcgagcg tggtgcgatc caacattatc gcgcggacca
    11101 caattcttcc agaggacaac tccacccctg tttttgatcc tgtcggcctt ctcagtcacg
    11161 gagctcgcac ggatcaaaga ggaagatttc gcacgtttgc gacgcgtttc ggagatctgt
    11221 tcattgcaac caacgacgct ccaggtgtga agctcgggaa aattctagac tattacaatc
    11281 gtatcgaggt tcattctgag ctagacccag aaagcagggc tgcgggaccg ctggtagcca
    11341 ccaattttgg tgtgatccgg gagagagttc ggcgcggcgc gaaatatggg gaaatgtccg
    11401 tcgttgctga agcgccgttg cttgaagatc agctaacgtt cctccgtgaa aatatttcga
    11461 ccagcgattt cattgcaatc gtatgctctc gcaagatgga tcgcaaatgc attccgtatc
    11521 tactccagag actggatcga aagcaatgga atgcaaatac tctagttgcg ctttggactt
    11581 tcaaagaacc tggcacacac gagaaaatcg aagcatacct cgcactgatt caatccaatc
    11641 aagaatgggt ggacgatgaa tgcttcgcga tccgcggatt ggtcgaaacg acgagcgaac
    11701 cggatgccaa agaccagaaa ctgatcgatc agtctatcct gcgataccgc gcattcgagg
    11761 gcaagttcgg cgagtcgggc cttgttgact acagagcgga tgatctggct ttcgcactca
    11821 taaaagctgg ccggtcagag ggactttttc tctgcataga cacaatcgag aagattgaaa
    11881 atcgattgga aacactcgca aactggggtg ttgccgtttc ctctacgatc ccggccggat
    11941 ttggaaatac acccgaggaa ctgggatacc gtttgcgctc cctgcaaaac atgctgaacg
    12001 aaagcactac ttttccagaa aagtacaacc accagcgccc tgtagaagaa cgcatgaaag
    12061 tgcgcgataa attcagggac tatgcgaaga aagcggagtt ttcacgcgca cgcttcagac
    12121 tcgcacaata gtgttctccc gttcaaccaa caaacggcgc ggctttaggt cgcaggctct
    12181 gcactacttc tgaaacctct gcatccgtca tgtacggaaa cagtggagga ttgagaatgc
    12241 tttctgcaag cttgcgtgca ttgtctccac cgactgctgc tttgatatag cctttcgcgc
    12301 cagcctgcga agacatactt ccgggatatg tatttgcaaa tccgatcccc ttctctttca
    12361 accgcgcttc tacagaagat ctctccgatg gattgtaaag aatgcagtaa agataaccat
    12421 tttcttcaaa tccttccggc ggacgaacag aaggaagttc ccccagatga gtgcggtatt
    12481 gtttcacggc cgctcttctg ctttcgatgc gagatttcac atgcggaatg gaaaggcgaa
    12541 tgtaagctgc ctgaaaagca gagatcctgg aattccaacc aaccagacca taagagta
//
LOCUS       DAOQJL010000038        12352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2543_length_12352_cov_4.122875, whole genome shotgun sequence.
ACCESSION   DAOQJL010000038 DAOQJL010000000
VERSION     DAOQJL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_12352_cov_4.122875"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLG78_02895"
     CDS             <1..228
                     /locus_tag="PLG78_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22084.1"
                     /translation="VGVKAMAGMLGVSIESQTRGVTHAFPVLSQDPRAIARFILDQWD
                     RIDWTILESFGLRRPEFNKWKSVCESRLSSI"
     gene            291..1352
                     /locus_tag="PLG78_02900"
     CDS             291..1352
                     /locus_tag="PLG78_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-arabino-heptulosonate 7-phosphate
                     synthase"
                     /protein_id="HNE22085.1"
                     /translation="MIIPKKSKLTEAQYQEVNTILSEFDCSITPIPGATRTIYAIRGD
                     ERHETMINRLEGLSYISKVDTVQSPYKLLDITASLATHRITIGGKTLGRDFLVVAGQC
                     TIDPKNPNYFYETAAGVKEAGAHVLRGGVWKPRTSPHSYQGNSKAIEILMQAHQKTGL
                     PVNTEVMDEEQLEIAVDAGAHMIQVGARNALNYSLLKKIGEKTSGKGTVILLKRGLHM
                     GPLSEFICAAEYIVAQGNPNVVFCPRGTVPGMDGYRNHPDESITPLLKQKTWAPVVVD
                     PSHSVGKAIYVPQCALAAVAYGADGICVETHCSPKNGIGDDPKQAIVPDVLKNLVIDA
                     AKLFEVTKKYQGYLVQSAE"
     gene            1855..3012
                     /locus_tag="PLG78_02905"
     CDS             1855..3012
                     /locus_tag="PLG78_02905"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE22086.1"
                     /translation="MEGAEFGGMRTAHSPIVVFGVILFGVLSSCSYSSSKMLTDDEVA
                     VLFLRQKYYSRFLFVVGAGDTTLRSFSIDNTTGSLTPAGIVSPSVAGIGIDIHPTLPY
                     VYQTLNGSDGIEAFRFDQTNAALTSVGVVTTGNNNSHRVRVLADGNSLFFNSVQCGNT
                     CVWRLPLSAGLPGTQAQVNNTIPNNSQWMDVDPRGRFLYSANNGGGFGINMFQINGSS
                     ALISQGSITINGGEQPSEGVFNSDGTALYALVQNTRVVAYSINQTTGVLSLLSSDPLP
                     GLPILMHPKGTFLYAFNTGSGIVRTYRINADKSFTVVTDQTAGGVSFDHVCMERGGRF
                     LFLTKFAAPGVILTYSIADNGALTPVATLTVSGSSQFMQCTTSIESRIGWF"
     gene            complement(3042..3626)
                     /locus_tag="PLG78_02910"
     CDS             complement(3042..3626)
                     /locus_tag="PLG78_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22087.1"
                     /translation="MNSDYLTKLIDSYDDKQLRRMVFSMDIQGKPLFKIFEDLPDPIA
                     RSVVSLLDMIVEYTMSEEFARAHSFKKGLRCFEELYHLYHSTDFLKNFNQFAEKNGFY
                     SDEDEAVPMINEDGSLEKLYKYVNIKHITPGFGRDQEELDLKMRNNQDFIKSMIPGDF
                     HIKADACVPVFGPVTAGRYERGLEGFRVKFVFKA"
     gene            complement(3638..5308)
                     /locus_tag="PLG78_02915"
     CDS             complement(3638..5308)
                     /locus_tag="PLG78_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22088.1"
                     /translation="MTQSKPRWAASGDWNAFFALFLDNIVNLVILSSILMLGFGFPKD
                     IIFHKILPGTALGVLFGDVVYSIMAIRLSRRTGQVVTAMPLGLDTPSTIGMAVAVLGP
                     AFLSLRGQGMSPDGAGDLAWKIGMASMLWMGGIKLIFSFIGATIQKVIPTAALLGSLA
                     GVGIVWLAADPLIHVYDLPFIGLIALAVLTFALIAKLELPFHLPGAAMAVVLGIVLYY
                     LLGELKLLPKDSMQVYKGFPAFEGIHFLLPFPTPAAFQEVFGRVIPYLPLAIPFGLLT
                     IVGGINVTEGARLVGDDFKTRDILLTEAFATIIAGVCGGVAQTTPYIGHSAYRQMGAR
                     SAYTLFTGIAVGLGASLGLVGFIVDLIPRAAVDPILIFVGLEILALSFRLTPKDHYMA
                     LGFALIPAILSFVHIKLKLLIQNVERVSGGISSTLSNLKPEQARPIQDALSQLIPVQI
                     SQEYQVIGVLGQGFILTSMVWAATVAYLIEKRFLAASIAMMAAAVFTFFGFMHSVFPD
                     GNVYLPWTIASQGISGRALAIPYELALCYIIMAALFPLLYAAQKKRAA"
     gene            5398..6435
                     /locus_tag="PLG78_02920"
     CDS             5398..6435
                     /locus_tag="PLG78_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE22089.1"
                     /translation="MQYRELSNTGLLVSELCLGAMTFGGKDGIWGMIGNLDQKEVDEL
                     VGASIDSGINFFDTANVYAFGESEKLLGKALSSRRRDVIIATKVRGRMGPGANQTGLS
                     RVHIMQSVEDSLRRLGTDYIDLFQIHGLDVLADFEDTLRTLDDLVRSGKVRYIGASNL
                     AAWHLMKCLGISKYQGLEPFKSIQSYYSLAGRDLEREIIPLLKSENVGLLVWSPLAGG
                     LLSGKFTKSNQDQEGRRAKFDFPPANKEKTFNIVDVLIKIGAAHNTSAARVALAWVLA
                     QPAVTSVIIGAKRRDQLDDNIASTQLKLTEDELGQLDAVSALSPEYPGWMFQMQGSDR
                     LPGQTRTIPKP"
     gene            complement(6466..6972)
                     /locus_tag="PLG78_02925"
     CDS             complement(6466..6972)
                     /locus_tag="PLG78_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE22090.1"
                     /translation="MAQKKKTPSKPKTAAKAARPRPTKPVFESRISFTTIGVRDFKRS
                     LDFYRDTIGFKLHSLQGDIAMFDVGGSVLALYPYHLLAEDAGLKPGGSGFSGIAMAYN
                     VRNAKDVDRFLGLLESRGTRILQKAEDKFWGGRSGYFADPDGYPWEIAWNPFIKMDAK
                     GRLILKKK"
     gene            7072..8124
                     /locus_tag="PLG78_02930"
     CDS             7072..8124
                     /locus_tag="PLG78_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22091.1"
                     /translation="MDSFTPIKDFAGARSLPHPGDRLSEIKKRSRKFRDKLLAGKKAE
                     FYKTVDLVRVPYPTKYGLLNAFALPTPMMHILNRLYIVQFKQKGRLRTLLFSPSDIEN
                     NGETPFFKRLSRSFGPFESIGKRFIAPVLSTVEHALQSIGLSPEKIDYISYDHLHTQD
                     VRKWLGTRNMAPYFPNAKLLVMKQEWDSTKSLIGPQLDWYCPGGIEGIDPRKVILLKG
                     DTMLGDGVALIATPGHTEGNHSLVVHTDEGVLVSSENGVSADSYAPMSSRIPGVRRYA
                     AKAEMEVILNGNTLEGGLDQYISMVQEKEIAGPSARNPDFYNVVPSSELTHYWGFPGI
                     RPTFTFGPLEFGKIRT"
     gene            8135..8950
                     /locus_tag="PLG78_02935"
     CDS             8135..8950
                     /locus_tag="PLG78_02935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22092.1"
                     /translation="MRDKVVIITGSGSGIGRHLTGAFLNSGALVAATDINEDALKKAA
                     KKDGWDKERLISEKLDVRQEKSWKKLFSQVLKKWKRVDILINNAGYLMPAYIADADED
                     HFARHLDINARGTMIGTAHAARVMMEQKSGHIINIASLAGVAGIPGISAYSASKHAVR
                     GFSLAAAQEMRPHGVFVTTICPDAVKTPMLDLQKDYKEAAMTFSGGRYLTPVDIEGAI
                     RRAIDTRQREILLPGYRGMLAKIASAFPSLTATLGGVMQKQGQKKQAAFRKGS"
     gene            8947..9267
                     /locus_tag="PLG78_02940"
     CDS             8947..9267
                     /locus_tag="PLG78_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22093.1"
                     /translation="MTEKEKKEDKGTNLLQTMGESLSNIADTLTGRHLENRIREYTEL
                     YADVLLGLHRDLRNLEGRIEALPDKKEMDSMLKGLQIMRRLLIVSLGFSIISLGGVIW
                     TILR"
     gene            9342..9611
                     /locus_tag="PLG78_02945"
     CDS             9342..9611
                     /locus_tag="PLG78_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22094.1"
                     /translation="MDREEDSAIKTALVKTGHTLLQLGIVRLAVRFFPGLTGFLTPAV
                     SVAYERVRKNTDLPGVVDQATAKLKNIQNERSVETTAEHKDSTHH"
     gene            complement(9597..10247)
                     /locus_tag="PLG78_02950"
     CDS             complement(9597..10247)
                     /locus_tag="PLG78_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE22095.1"
                     /translation="MGAKNKSVLDIGCGQGVYSRALAEAGARVTGIDASPSLIEAARR
                     YQSKVPVKYECENAEDLRAFADQSFDQVSAILCIQNMRNLDRVTSECARVLRPNGRML
                     WVLNHPCFRIPRQTSWGFDEGKKMQYRRIDMYMSPAEIPIQMHPGKHGTESTVSFHRS
                     ITDLFSIASRFSFAAVKVEEWSSTRSSQPGPRAKAENRARKEFPLFMAVLFQLVVG"
     gene            complement(10364..10849)
                     /locus_tag="PLG78_02955"
     CDS             complement(10364..10849)
                     /locus_tag="PLG78_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22096.1"
                     /translation="MAAASLLLAAAGFHFSQINHSWNGFRPMGQETVRTGSEASEITL
                     LSGFSTRGRIHLKQDTELSRHGSGHYELHQGQAEVHVHPQGSTVTLWANGIELASQDG
                     VFTIVTGGEEHPQGSVTLTVSRGSVECCQNGENFPILPGEKITLVKRDADAQVLAYES
                     E"
     gene            complement(11099..11611)
                     /locus_tag="PLG78_02960"
     CDS             complement(11099..11611)
                     /locus_tag="PLG78_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE22097.1"
                     /translation="MYELREYCAAIFAEHSGKLLRYLRRLGGGANSEDILQEVFIRFI
                     REVQHDRIQKGSELRWLYRASRNLAIDYYRKDRKNTPWTDDRIAATGEGEGKIVLGSE
                     LVSRMFDVARKCGPDYETLLHLILETRESQSSMAAILKCSERTVRRRTETLFGALERG
                     LPDWRDYLET"
     gene            11738..11947
                     /locus_tag="PLG78_02965"
     CDS             11738..11947
                     /locus_tag="PLG78_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22098.1"
                     /translation="MKRLRLIAPAVAMALATTLVAGPKEDCLQKAKTDHRAAHKECKA
                     KDGQARKDCQKAAKTAFETARKACK"
     gene            complement(12015..>12352)
                     /locus_tag="PLG78_02970"
     CDS             complement(12015..>12352)
                     /locus_tag="PLG78_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNE22099.1"
                     /translation="EILTSGHLRVHSGDTDLYGVKILLAGGHTPGSQAAVVQTSNRAF
                     IITGDECYFADACVAQIPLPEESAFSPEKNQKFIQRVGSQNWRILTLHDPAVFSAYDA
                     RLRIVRLTQ"
BASE COUNT         3230 a         3266 c         3067 g         2789 t
ORIGIN      
        1 gtaggtgtaa aggccatggc aggaatgctc ggcgtttcca tcgaaagtca gacccgtggt
       61 gttacacatg cttttccggt tctttcccag gaccctcgtg cgatcgcccg gtttatactc
      121 gatcaatggg accgcataga ctggacgatt ctagaatcgt ttggtctgcg acggcctgag
      181 ttcaacaagt ggaagagcgt ctgcgaatcg cgcctatcat ctatataaga aatagataaa
      241 cctataaaag aatcgcccgt cttcctgccg atatttttgt tggaattgaa atgatcatac
      301 ccaagaaatc gaaactgaca gaagcgcaat accaggaagt caacacgatc ctgtcggagt
      361 tcgactgtag catcacgcca atccctggcg cgacgcgcac catttacgcc attcgtggag
      421 atgaacgcca cgaaacgatg atcaacaggc tcgaaggcct ttcctacatc tccaaagtag
      481 acacagttca gtccccgtac aagctccttg atattacggc atctcttgca acgcacagaa
      541 ttacgatcgg tggcaagact cttggccggg atttccttgt agtggcggga cagtgcacca
      601 tcgatccaaa gaatccaaat tacttctacg aaactgcagc cggtgtgaaa gaggcgggcg
      661 cgcatgtact tcgcggaggc gtctggaagc ctcgcacgag tccgcattcc tatcagggaa
      721 attcaaaggc gattgaaatt ctgatgcagg cacaccagaa aaccggtctt ccagtgaaca
      781 cggaagtcat ggacgaagag caactggaaa tcgcggtcga tgcaggcgca cacatgatcc
      841 aggtaggagc gcgcaacgca ctcaactact ctctgctaaa aaagattgga gaaaaaactt
      901 caggcaaagg cacagtcatc ttgctcaagc gcggacttca catgggccca ctctctgaat
      961 tcatctgtgc tgcagaatac attgtagccc agggaaatcc aaatgttgtc ttctgcccgc
     1021 gcggcaccgt tccaggaatg gacggctaca gaaatcatcc agacgaatcc atcacaccgc
     1081 ttctcaaaca aaaaacatgg gcgccggtcg ttgtggatcc atcgcattcg gttggtaagg
     1141 cgatctatgt accgcaatgt gctcttgcag ccgttgcgta cggcgctgac ggcatatgcg
     1201 tggaaactca ctgttctccc aagaatggga tcggagacga tccaaaacag gcgatcgtgc
     1261 cagatgtgct gaagaatctt gtaatcgatg ctgccaaact gtttgaagtg actaagaaat
     1321 accagggcta cctggtgcag tctgcagaat aatcgcctcg ggcggccggc agcgagttag
     1381 ctgcaacgcc cgggatgcag tccctgctcg cgctgaagcg gcgctcggac gctccgacct
     1441 ccgctgtgga ctgcacccgg gcgtcgcaac acagatcgcg ctgagccttc gaggctttca
     1501 ttttcgccgc gcatttcata aacgattcgc gcagcgaatc gaaccccatc gcgaccggtt
     1561 tatcacgcaa tgaatgacgt attgcatgca cacgcacgca gtatcacgca ctcttcggcg
     1621 ttccgtttgg ggagcgcgga cgttatgcgg catatcgaag cagtcttgga cggcaggcgc
     1681 gggcgttttc aatcgaaaag attcccgata acgattcgcg cgtcgtgtca atcctccacc
     1741 tatggctgtg cgaaccgatg atctctctgc gcttacccaa ttctgggcag gacggagcgg
     1801 ttgccgagtg caagcggcag cgacggaatt tcgaaaaagg agctgacaaa ccccatggaa
     1861 ggagccgaat ttggagggat gcggactgct cactctccga tcgtggtttt cggagtaatt
     1921 cttttcggag ttctctcgag ctgctcctac tcctcctcaa agatgctgac ggacgacgag
     1981 gttgccgtcc tgttcctccg ccagaagtac tacagccgtt ttttgttcgt ggtaggtgcc
     2041 ggagacacaa cgctgcgctc ttttagcatt gataatacca cgggaagcct gacaccggcc
     2101 ggtatcgtct ctccttccgt ggcgggcatt ggcatcgaca ttcatccgac tcttccgtat
     2161 gtataccaga cgctgaacgg atcagacgga atagaggctt ttcgattcga ccagaccaac
     2221 gcggctctca cgtcagtggg cgttgtaacc acgggcaaca acaactcgca tcgagtgcgc
     2281 gttctcgcgg acggcaattc ccttttcttc aactccgttc aatgcggcaa cacgtgtgtg
     2341 tggaggcttc ccctgtccgc cggactgccg ggcacgcagg cccaggtgaa caacacaatt
     2401 ccgaacaaca gccagtggat ggatgtcgat ccgcgaggaa gatttctgta tagcgccaac
     2461 aatggcggtg gtttcggcat caacatgttt caaatcaacg gttcgagcgc attgatttcg
     2521 caaggcagca ttactatcaa tggaggcgaa caaccttctg agggcgtttt caactctgac
     2581 ggcaccgcat tgtatgcact ggtgcaaaat acgcgagtcg tcgcttactc catcaaccag
     2641 acaacaggtg tactcagcct gctctcgagc gaccccctcc caggacttcc cattctcatg
     2701 cacccaaagg gaacattcct gtatgcattc aacacaggaa gcgggattgt aaggacatat
     2761 cgcatcaacg cggacaaatc gttcaccgta gtcacagacc agactgctgg cggtgtttct
     2821 tttgatcacg tatgtatgga gcgaggcggg cgctttctgt tcttgacgaa atttgcagca
     2881 cccggagtga ttcttacgta cagcattgcc gacaacgggg ctctcacccc tgtcgcgaca
     2941 cttactgtgt cagggtcaag ccagttcatg caatgcacca cgtcgattga aagccggatc
     3001 ggctggttct agatccaggc caagcgcgaa attccttagc actaggcctt gaatacgaac
     3061 ttcacgcgaa atccttcgag accacgttcg tatctacctg ctgtgactgg cccgaagact
     3121 ggaacgcagg catccgcttt gatgtgaaag tctccaggga tcatgctctt gatgaaatcc
     3181 tgattgttcc tcattttcaa atctaactct tcctgatcgc gaccaaaacc aggagtgatg
     3241 tgcttgatat ttacatattt gtaaagtttc tcaagtgatc cgtcttcatt gatcatgggc
     3301 actgcttcat cttcgtcaga gtagaaccca ttcttctccg caaactgatt gaagttcttc
     3361 aggaaatcgg tggaatgata aagatgatac agctcctcga agcaacgcag acccttcttg
     3421 aaactgtgcg cgcgcgcgaa ctcttcgctc atcgtatatt caacaatcat atccaggaga
     3481 gaaacgacgc ttcgcgcgat tggatcgggt agatcttcga aaatcttaaa gagaggcttt
     3541 ccctggatat ccatggaaaa aaccatgcgt ctgagttgct tgtcgtcgta ggaatcgatc
     3601 agttttgtca aatagtcgct gttcattttt cttctcctta tgccgcccgt tttttttgcg
     3661 ctgcatacaa cagcggaaag agcgcggcca tgatgatgta acagagagca agttcatatg
     3721 gaattgcaag cgcgcgtcct gaaattccct gcgaagcgat cgtccaggga agatacacgt
     3781 tgccatccgg aaagacggaa tgcatgaatc caaaaaaagt aaaaaccgcg gcggccatca
     3841 tcgcaatgct tgctgccagg aatcttttct caatgagata ggcaaccgtg gcagcccaga
     3901 ccatggatgt aagaatgaat ccctgtccaa gcactccaat cacctgatat tcctgagata
     3961 tctgtaccgg gatgagctgc gaaagagcgt cttgaattgg ccgggcctgt tctggtttca
     4021 gattgctcag agtagaagaa attccgccgc tgactctctc tacgttctgg atcagaagct
     4081 tcagcttgat gtgcacgaaa ctgagaatgg caggaatgag cgcaaagcca agtgccatgt
     4141 aatgatcttt cggagtgagc ctgaaactga gagccagaat ttcaagacca acgaagatca
     4201 aaatcggatc cacggctgct cgcggaatca aatccacaat gaatccaacc aaccccagac
     4261 tcgcgcccag gcccactgca atcccggtga agagagtgta tgcgctgcgc gctcccatct
     4321 gcctgtatgc tgaatgaccg atgtatggag tagtttgcgc gacgccgcca caaacgcccg
     4381 caatgattgt tgcgaaagct tccgtaagta gaatatcgcg cgtcttaaaa tcgtctccaa
     4441 ccagacgcgc gccctctgtc acgttgatcc cacctacaat cgtcaaaagt ccgaacggaa
     4501 tggcgagagg caaataggga atcactctac caaacacttc ttgaaaagca gccggtgtag
     4561 gaaacggaag aaggaagtga attccttcaa acgcagggaa tcccttgtag acttgcatgc
     4621 tatctttcgg aagaagtttc aattctccca gaagatagta gagaacaata cccagaacaa
     4681 ctgccatcgc cgctcctggc agatgaaacg gaagctctag ctttgcgatg agtgcgaaag
     4741 tcaataccgc aagcgcgatg agtccaatga atggcaaatc atatacatga atgagcggat
     4801 ccgccgcaag ccacacaatg ccaactcctg caagagaacc aagcaacgcc gctgttggaa
     4861 tgaccttctg aatcgtggcg ccaatgaaag agaaaatgag tttgattcca cccatccaga
     4921 gcatgcttgc cattccaatt ttccacgcca ggtcccccgc accatccgga ctcatcccct
     4981 gaccgcgcag actcaaaaac gcgggaccaa gaactgcgac agccattccg atcgtcgatg
     5041 gagtatccag tcccaatggc atggccgtga ccacctggcc cgtgcgacga gacagtcgaa
     5101 tggccatgat gctgtagacc acatctccga agagcactcc gagagctgtc cccggtagaa
     5161 tcttatgaaa gataatgtct ttgggaaaac caaatccaag catcaagatg ctagatagga
     5221 tcaccagatt cacgatgtta tccaggaaca gtgcgaaaaa tgcattccag tcgccggatg
     5281 cagcccagcg cggcttcgat tgcgtcatgg agactcactg gaaattctgg ccgggcgcgc
     5341 aactgtaaat tcagagccgt gctcgcccgg gcgagccata tgtattttct tatcaaaatg
     5401 caataccgcg aactatccaa caccggtctt ctcgtttctg aactctgtct gggtgctatg
     5461 acattcggag gaaaagatgg aatctgggga atgatcggca acctggatca aaaggaagta
     5521 gacgagctcg taggagcatc gatcgattct ggaatcaatt tcttcgacac tgcaaacgtg
     5581 tacgcgtttg gagaatcaga aaaactcctg gggaaagcac tctccagtcg cagacgcgat
     5641 gtgatcatcg caaccaaggt gcgtggacgc atggggccag gtgccaatca aacagggctt
     5701 tcgcgcgttc atatcatgca atctgtagaa gacagcctgc gaagactcgg cacagactac
     5761 atcgatctgt tccagatcca cggactcgat gtgctcgctg attttgaaga tactctgcga
     5821 actctggacg atcttgtgcg cagcggcaaa gtgcgttaca tcggggcatc gaacctggca
     5881 gcatggcatc tgatgaagtg tcttggcatt tcaaagtatc aggggcttga accgtttaaa
     5941 agcattcaat cctattactc tctagccggg cgggacctgg aaagagaaat cattccactt
     6001 ctaaaaagcg aaaacgtcgg tctgcttgtg tggagtcctc tcgcaggcgg gctactgtct
     6061 ggaaaattca cgaagtccaa tcaggatcag gaaggaagac gagccaaatt cgatttccct
     6121 ccagcgaaca aagagaagac gtttaacata gtagacgtac tcatcaaaat cggtgccgcg
     6181 cacaatacgt ccgccgctcg cgtagcactt gcatgggtgc tcgcacagcc ggcagttaca
     6241 agcgtgatca ttggagccaa aagacgcgat caattggacg acaacattgc ctccactcaa
     6301 ctgaaactga cagaagacga actggggcaa ctcgacgcgg tcagcgcact ttcgcctgag
     6361 tatccgggat ggatgtttca aatgcagggt tcagatcgcc ttcctggtca gacacgaacg
     6421 attccaaaac cctgagccaa cgtttctgag gtaggtgcgc gagggttatt tcttctttaa
     6481 gatgagtctg cccttcgcgt ccattttgat gaacggattc caggcgattt cccacggata
     6541 accatccgga tctgcgaagt agcctgaacg cccgccccag aatttgtctt ctgccttttg
     6601 taagatcctg gtcccgcgag attccagaag gccaagaaat cgatccacat cctttgcatt
     6661 acgaacgtta tacgccattg caatgccgct aaacccggac cctcctggct ttaatcccgc
     6721 atcctccgca agcaaatgat acggatacag agcaagaaca gaacctccta cgtcaaacat
     6781 cgcgatatcg ccctgcaatg aatgaagttt aaaaccgatt gtgtcgcggt aaaagtcaag
     6841 ggatcgtttg aagtcgcgca ctccaatcgt tgtaaaacta attctagatt caaacaccgg
     6901 tttggtgggt ctgggcctgg ctgcctttgc agcagtcttt ggtttagatg gagttttttt
     6961 cttctgggcc atagtctcaa aggtatagtg tgcgctcgtt gtcaacttct acagattcga
     7021 ctttggcttg acctgctcta gcatgcgcgc ccccttccca tcaggcacac tatggattca
     7081 tttacaccca tcaaagactt tgcaggcgcg cgctctcttc cgcatccggg agatagactt
     7141 tctgaaatca aaaagagatc acgtaagttc agggacaaac tcctggctgg aaagaaagca
     7201 gagttctaca aaacggtaga cctggtgcgc gtcccctatc ccacaaaata cggccttctc
     7261 aacgcctttg cccttcccac tcccatgatg cacatcctta acaggctcta cattgtgcag
     7321 ttcaaacaaa agggaaggct tcgcacgctc ctattttctc cgtcagacat tgaaaacaac
     7381 ggagagactc ctttcttcaa aagactttcg cgctccttcg ggccgttcga atcgatcgga
     7441 aaacgtttta ttgcaccggt gctttccact gtagaacatg cgctacaatc cattggactt
     7501 tcccccgaaa agatcgacta tatttcttac gatcatctgc acactcagga cgtacgcaaa
     7561 tggcttggca cgcgcaatat ggcgccttac ttcccgaatg cgaaactact tgtgatgaaa
     7621 caggaatggg actccacaaa aagcctgatc ggaccgcaac tcgactggta ttgccccggt
     7681 gggattgaag gaattgatcc gcgcaaagtc attctcctca aaggagacac tatgctcgga
     7741 gacggagttg ctttgattgc cacaccagga catacggaag gaaaccactc cctggtagtt
     7801 cacacagatg aaggagttct ggtctcaagc gaaaatggcg taagcgcaga cagttacgcg
     7861 cccatgtcgt ctcgcatccc tggtgtgaga cgttatgcgg caaaggcaga aatggaagtg
     7921 atcctgaatg gaaacactct cgaaggtgga ctcgaccagt acatctccat ggtgcaggaa
     7981 aaggaaattg cgggaccttc cgcacgaaat cctgacttct acaacgtggt tccttcctcc
     8041 gaactcacac actactgggg atttccaggc atccgaccaa cattcacatt tggccctctc
     8101 gaatttggaa agatacgaac gtaaggagtt catcatgcgc gataaagtag tcatcatcac
     8161 gggttcagga agcggaatcg gaagacacct gacaggagcg tttttgaact ctggcgctct
     8221 cgtggccgcc acagacatca atgaagacgc tctcaaaaag gcagcaaaga aggacggctg
     8281 ggacaaagaa agattgatct cagaaaaact cgacgttcga caggaaaaga gctggaagaa
     8341 gctcttctcg caagttttga aaaaatggaa gagagtggac atactcatca acaatgcagg
     8401 ctacttgatg ccggcctaca ttgcagacgc agacgaagat catttcgcgc gccacctgga
     8461 tatcaacgcg agaggaacca tgatcggcac agcgcatgca gccagagtga tgatggagca
     8521 gaagtcaggt catatcatca acatagcttc gcttgcagga gtggccggaa tcccgggaat
     8581 ctccgcatac tccgcatcca aacatgcggt gcgcggtttt agcctggcgg cagcccagga
     8641 aatgcgccca cacggcgtat tcgtcacaac catctgcccc gacgccgtga agacaccgat
     8701 gctcgattta cagaaagact ataaagaagc ggcgatgacc ttttctggcg gccgttatct
     8761 gaccccggta gacatagaag gagccatcag acgtgcaatt gacaccagac aaagggaaat
     8821 cttactgcct ggatacagag gaatgcttgc caaaatcgcc tctgcgttcc cttctttaac
     8881 agcaaccctt ggtggcgtga tgcaaaagca gggacagaaa aagcaagcag ccttcaggaa
     8941 aggatcatga cggaaaagga aaagaaagaa gacaagggca ccaatctgct acagacgatg
     9001 ggcgaaagcc tttcgaacat cgcggacact ctgaccggtc gtcatcttga aaacagaatt
     9061 cgcgaatata cggaattgta tgcggatgtt ctcctgggcc ttcatagaga ccttcgcaac
     9121 cttgagggca ggatcgaagc gcttccggac aaaaaagaaa tggattctat gttgaaaggc
     9181 cttcaaatca tgagaaggct tctcatcgta tcgcttggct tttctatcat tagcctcgga
     9241 ggtgtcatct ggacgatcct tcgctaaaag aaaaactcac tgagctcgaa acgcgacgcg
     9301 gaacggggga gaaatttcgc gacgttgcca gacagtatgt aatggacaga gaagaagaca
     9361 gtgctatcaa aacggcactt gtcaagacag gccatactct gctgcagctt ggaattgtgc
     9421 gcctcgcagt ccgattcttc ccgggtctga ctggattttt aacaccagcc gtttccgtgg
     9481 cctatgaacg agtgagaaaa aacacggacc ttccgggagt agtggatcaa gccactgcca
     9541 aactcaaaaa tattcagaat gaacgcagcg tcgaaactac cgctgaacac aaagactcaa
     9601 cccaccacta actgaaacag gactgccata aagagaggaa actctttgcg cgcgcgattc
     9661 tctgctttcg cacgcgggcc aggctggctg gatcttgtgg atgaccattc ttccacttta
     9721 acggccgcga aagaaaagcg cgaagcaatc gaaaatagat ctgtgatgct tctgtgaaag
     9781 gaaacagtag attcggttcc gtgttttccc gggtgcattt gaattggaat ttccgcggga
     9841 gacatgtaca tatcaatcct gcgatattgc attttctttc cttcgtcaaa tccccaggat
     9901 gtctgtctgg gaatgcgaaa gcaaggatga ttcaagaccc atagcatcct tccattgggc
     9961 ctcagtacgc gcgcacattc ggatgtaacg cgatcaagat ttctcatatt ttgaatgcaa
    10021 agaatcgcgc tcacctgatc gaaagactga tccgcgaatg cgcgaagatc ttctgcattt
    10081 tcgcattcat actttacagg gacttttgat tggtaacggc gtgcggcctc aatcagagaa
    10141 ggagacgcgt ctatgcctgt aacgcgcgct ccggcttccg caagagcccg actgtatact
    10201 ccctgaccac aacctatatc aagcaccgat ttattttttg cgcccatcag cgcgagcgcg
    10261 cgagggagta tgatttcgcg atgtaaatcc gaaccactct tgcctacaag gccgtcatac
    10321 cagtctgaag acttctccca ggaagtatca gaccgttcct gtgttattcg ctttcgtatg
    10381 cgagaacctg ggcgtctgcg tcgcgtttta caagcgtgat tttttccccg ggcaaaattg
    10441 gaaagttttc cccgttctgg caacattcta cgctaccacg agatacggta agtgtcacgc
    10501 ttccttgagg atgctcttct ccaccggtga caatcgtgaa cactccatcc tgacttgcca
    10561 gttcgattcc gttggcccag agagttacag tgcttccctg gggatgaacg tggacttccg
    10621 cctgcccctg gtgcagctcg tagtgtccgg acccatggcg agaaagttcc gtgtcttgct
    10681 tcagatgaat gcgtccacgg gtagaaaacc cggatagaag agtgatctcg ctggcctcag
    10741 agccagttcg caccgtttcc tgacccatag gacgaaagcc gttccagcta tgattgatct
    10801 gggaaaagtg aaagcctgcg gcagccagga gcaaactggc tgccgccaaa cccggataga
    10861 acgtatggtt cgcgcgcgtc cggggggacg tagcgcgcgc aaacgcctgc tcaaagatcc
    10921 aattcggcgc cgcaggaaga tccggagtac tggtttcctg ggtgctgttc cggttaaggg
    10981 aaggagtcgc ccgcccggga atcgagtcga tgaatcgtcg gtgcaaaacc tgactcagac
    11041 tgccatcgtc tttcacactc tccttacgca cattccacct ccggggggcc aaaaaaaatc
    11101 atgtctcgag gtaatcccgc cagtctggca gtcccctttc cagggctcca aaaagggtct
    11161 ctgtcctacg acgaaccgtt cgttctgagc atttcaaaat agcagccatg gacgattgag
    11221 actcgcgtgt ctccagaatc aaatgtagaa gggtttcata gtccggtccg cattttcggg
    11281 caacatcaaa catcctggaa acaagctcag aaccaagcac gatcttccct tctccttcgc
    11341 ctgttgccgc gattcggtca tccgtccagg gggtgttctt tcgatcctta cgatagtagt
    11401 cgattgcaag attacgagaa gcacggtaca gccatcgtag ctccgaaccc ttttgaatcc
    11461 gatcgtgctg cacttcgcga atgaagcgaa tgaacacctc ttgcaaaata tcttccgaat
    11521 tcgcgccgcc tccaagccga cgcaaataac gcaacagctt acctgaatgt tcagcaaaaa
    11581 tcgcggcaca atattcgcgc agctcgtaca caaaaccagt ctacccgaag ctccgtgcgc
    11641 gtcatttctt tggcgcaaca ttgctcttca tttttttttt gaccccgcag gcggccattg
    11701 tccgtatatc tacggaatct aaaaccatga gggaatcatg aaacgactca gactgattgc
    11761 accagccgtt gcgatggctc tggcaacgac tctcgttgct ggcccaaagg aagactgctt
    11821 gcagaaggca aagaccgatc atagagccgc gcataaagaa tgcaaggcga aagatggtca
    11881 ggctcggaag gattgtcaga aggcggcgaa gaccgctttc gagactgcca ggaaagcttg
    11941 taaatgagta cgggccgccc gcgggcggcc tttcttttta tcgcagagaa cgattctgaa
    12001 aaactgcgcg gtcgttactg agtcaaacga acaattctca aacgggcgtc gtatgcgctg
    12061 aacaccgctg gatcatgtag cgtcaggatc cgccaattct gtgaacctac tctctgaata
    12121 aatttctgat tcttttccgg agaaaaagcg gattcttccg gaagtggaat ctgtgccacg
    12181 caagcgtctg cgaaatagca ctcatccccg gtgatgatga acgcccggtt gcttgtttgc
    12241 acgaccgccg cttgcgaccc aggagtgtgg ccaccggcga gtagaatctt cacgccatac
    12301 aagtctgtgt ccccggagtg tacacgcaaa tgtccgcttg ttagaatttc tt
//
LOCUS       DAOQJL010000039        12278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2569_length_12278_cov_4.202733, whole genome shotgun sequence.
ACCESSION   DAOQJL010000039 DAOQJL010000000
VERSION     DAOQJL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12278
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_12278_cov_4.202733"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /gene="ilvN"
                     /locus_tag="PLG78_02975"
     CDS             <1..257
                     /gene="ilvN"
                     /locus_tag="PLG78_02975"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE22100.1"
                     /translation="DSLIRELVLVRIKSDGSKRNEIFGIVEVFGGKVAEVTEDSILIE
                     IHGSGRQIHAAIDILAQFGIIEMARTGAIALAYRTDTGAG"
     gene            complement(259..1446)
                     /locus_tag="PLG78_02980"
     CDS             complement(259..1446)
                     /locus_tag="PLG78_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22101.1"
                     /translation="MRFYFRISVAALAVSLLLCKGGNSPASARENSESHREWNDIARF
                     LAGMEPDAGSTISDLEGLPAAESQRAYFAKIWPEVEKTQLEPQRKWRDEVLGKTEGFI
                     FYPFSGADFLNIYTFFPQGSEYVFFGLEPPGPPPSVKGMKPADVNQSLAGLQVSLRSI
                     LNYSFFQTLHMVNDLKATKLSGVAPVLLVFAARTGNKVHSVHNIRLHPDGKIESLPDP
                     FSGKPEPAATSEDYVSGIRVVFQKPGATEYTHMDYFSIDISEEGLAKKPYFLKYIQSL
                     GTPTTYLKAASYLMYNKDFTTIRNFILDESKLILQDDSGIPLQYFKPQSFDLTFYGSY
                     TRPIPLFDGKYQKDLYEMYLPKNHPKPLPFGIGYKFRKGDSNLMLAKRKTDRPDIPAP
                     SKQ"
     gene            1555..3093
                     /locus_tag="PLG78_02985"
     CDS             1555..3093
                     /locus_tag="PLG78_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE22102.1"
                     /translation="MNNTEALARRLGFWSASIIVVANMIGSGIFGNTGYIQAQVGSPI
                     MVMALWTLGGLIALAGALCYAELSTTYPHAGGEYVYLKHIFGGLPSFLTGWISLLVGF
                     AAPAAAAALLSGVYMSDFLKVVYPDTILSRFLLDETNRKILASALIILFGIFHSFGVK
                     QSGRVQNFLTGSKIIVIILFAVGGLTLAHRNPGIPIHQAFSGAVKPEGLGVGLMFVMF
                     AYSGWNAASYLAEEIKQPEKNLPRSLIAGTLFTIIIYLVMNYIYYLAVPATELAGKPD
                     PAAQAANHLFGEKTNRIFHVAFSFMLLSTLSACVMIGPRVYYAMSRDGLFFKFAGQLS
                     HTGVPVRSIFVQCFISLLYILLGTYEDIQTYMGFSLSLFPVLTVFGLILHRKRFPKLE
                     RAYKSPLFPFLPIMFILLSGLIVLTSYLDRPREGNFALSVVLAGVPMYYLWMRLRAGN
                     STHLLALITLQVLCVLAAGVAYHVLKYNPDALHMSLAALGVEVISVAVFSYWLRRQYQ
                     SLAR"
     gene            complement(3035..3595)
                     /locus_tag="PLG78_02990"
     CDS             complement(3035..3595)
                     /locus_tag="PLG78_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNE22103.1"
                     /translation="MSDRQYPARQYFSLDIPRKGPPVAAQPGILRVRRFRVSPRFDTR
                     SLVYRTGDVQYDTDFYNEYLTDAGAMVSEAARNWLFQAGSFATVVDSSSSLSEDFVLE
                     GVIQNLSGDFRSSPRAEVSVQLFLISSSATNGGVVFQKLYNRSIALPEKSPRALVVGL
                     GSGLSEIFTELETDIVSAASKRTQPH"
     gene            complement(3640..4578)
                     /locus_tag="PLG78_02995"
     CDS             complement(3640..4578)
                     /locus_tag="PLG78_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE22104.1"
                     /translation="MKSKTRYYKIGLFVMSGFLLLSVGLVLLGAGSFFKKTIRMETYF
                     DESVQGLDVGSAVKHRGVQIGTVESIGFVRNTYGELSEDDYYKYGKYVLVRIALTDPL
                     GMRGQDDTILQKMIQDGLRVRLTSQGLTGTAYLELDYLPPDQNKPLPIVWAPRSVYVP
                     SAPSTITRLSANIDRFFRKLEDSNVENLAVDLDRFLISGTKAVDDAKIPELRAEAVAL
                     LGEVRKTNNSFRELLSSPETKSIPENVGGSLAKLNQTMSRIDQLLTRNQSEMEETLEN
                     LKTASRDMKELTGTAKRYPSLLLFGTAPEKSTSFSH"
     gene            complement(4575..5339)
                     /locus_tag="PLG78_03000"
     CDS             complement(4575..5339)
                     /locus_tag="PLG78_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE22105.1"
                     /translation="MVMEANLEPPFIRVSDLRVMYDGTTILENVNFEVGRGEILFIIG
                     GSGCGKSTLLKSMVGLVEPDPGSVVFGDRDLVGATGDDRSAILRRFGVMYQGGALLGS
                     LTLLENVRLPLEEYSGLPEEAQDLLALMKLKLVGLEGYDHHFPSRISGGMLKRASIAR
                     AMALDPEILFLDEPSAGLDPVTSFELDRLIYFLSRNLGITFVIISHELSSIQKIADRV
                     IMLDKSARGIIAVGKPGDLAASTVPRVKQFFSRGEA"
     gene            complement(5333..6439)
                     /locus_tag="PLG78_03005"
     CDS             complement(5333..6439)
                     /locus_tag="PLG78_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNE22106.1"
                     /translation="MSVRFEVSGDSLRITFDGPLTFETAGSMFPEIMHKLKTGVRGIS
                     VDAHRVDAMDTAGAALLIHLEKHQSTLDLPFEIVGLAESYRKLYHLFDRAILEQTWED
                     EEIRWHFDAIGRRTVSLWEKVKEFTAFCGELFVALMKSISNPSRIRWKDVFITCESAG
                     VGALPLIGMIGFLIGLILAFQSAIPMRRFGAEIFVANLIGLSMIRELGPLMTSVILAG
                     RSGSAFAAEIGTMRINEEIDAIVTMGFDPMKFLVVPRIIASLIVAPLLTLFFQLAGLA
                     GGAVVMLSLGFPFVTYVQQVQSAAATSDLLGALFKVTVFGVLIAAIGCYRGLETKAGA
                     SAVGASTTSAVVSGIILIAVFDGIFAVIFYFMGW"
     gene            complement(6446..7252)
                     /locus_tag="PLG78_03010"
     CDS             complement(6446..7252)
                     /locus_tag="PLG78_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNE22107.1"
                     /translation="MRQALLVFLSFLCCNAPRSAPLNPPAAGLSTVDVGGVPCDVFVP
                     DAPVGTVLVLPGWNFDRGSWRKNSSIEEQARKHHLILILPDMLKTLYETSYYPETTLR
                     WNKTPGSQFIHDTLIPEMQKRYSLLMPGQRNFVLGLSTGGRGVAMTILENPKLFAAGA
                     AFSGDYAQERMPGEAINAAIWGPYALFPDRWKGRDNPARRASEWQTPIYLAHGLDDRV
                     VPPDQTQMFGDLLSARDKTLVEVHLKAGYGHDYRFWDSQLAPAFEFFSRH"
     gene            7479..7859
                     /locus_tag="PLG78_03015"
     CDS             7479..7859
                     /locus_tag="PLG78_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE22108.1"
                     /translation="MLRVRTSVRPSQIAGLGLFAAEDISKDQIIYEWTAKFEQTFTAE
                     EYEKLGPLQKEFLRHYSFKRSGIRVVSLDDDRFANHSDTPNTYEKGADTHALRDIAAG
                     EEITCNYYDFDESADEKLAGIQRS"
     gene            complement(7949..8284)
                     /locus_tag="PLG78_03020"
     CDS             complement(7949..8284)
                     /locus_tag="PLG78_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22109.1"
                     /translation="MAPADGTNGTPDLRGQFIRGRDDVGTGPAGMDPDGSRVIGALQA
                     DAFQGHVHQSRVWLGNPNNFLPAGGLQELSKDGPTGGPMSDGVNGTPRTANETRPKNV
                     ALTYCMRKN"
     gene            complement(8563..10038)
                     /locus_tag="PLG78_03025"
     CDS             complement(8563..10038)
                     /locus_tag="PLG78_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22110.1"
                     /translation="MKPKFKWCLGLLCLLALGCQKSKGDSSLSRLLMLSAAGSPIVRP
                     ASGATIQAPTMSLTVPQGALEEETTITYEPIDVPAGTDTIQPLQAAYKFGPENLQFSK
                     PATLQICYDARNVNERGLQEKTMQIQYFDEESGQFVSMGGEVDQASHCVSAPIYHFSS
                     YILTAQLLSAGNNAPTIGGAQFFPGRVMAGQPVTVRSTVTDWDGGSGIATVRFFYRTT
                     GSGAAFKSVVMIPDSNDGSGQFYTAKVPGADVTSAGLQYYIQAFDTLNAGRTNPATAP
                     VALGTVPGDTVFAAAPIRFQTPVSQISAGFSRDLTVQVRGNASATYYPVAAETLTFAG
                     AKGTTARPTWLSARYTAQKIGSSFLEATYGTLNLSVPINVYPGVLSRIEVLYNDAVLS
                     DPFNVNAGTVKQLDAAGYDSFDNFMFVQPVFSVAGGIGSFGGPANYGQFTAAPLPADT
                     TGTITATLGGGFSVTYNVLVHGSGASCQFDVGHFDTSCVFN"
     gene            complement(10173..11471)
                     /locus_tag="PLG78_03030"
     CDS             complement(10173..11471)
                     /locus_tag="PLG78_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE22111.1"
                     /translation="MNDSQREELARRLLEQAPASWPEELRALRMDLRAMPEGLRSKLA
                     AMHGQKESRASFKTKVIRWPVAAAVSFSGAIAAALVVYFGVFRVDVPPMRIVFSDGDT
                     RKEGQPVKTGTLGEHERITVGDASSLVLGSADKKTEIGLRVRANSELTLPVLQADLVE
                     ARLVRGIVLSQLSRKDSPARALLISTHNAQTRVTGTVFSVDVSRSSDTSLSTFEGTVS
                     IRRRWSDLEDLPENLLQRSEILSQSRDVFLKASASVPAGSQAVVSGMDFERRLGAIPH
                     LRKILSDPRISALRGRLKVSDSEVETAKKIIESNFPGPREREENLRLVETAFGQPPEV
                     TVVPAEDLSAMRQSLEQPSEAERKVRYEALLKQNPAPDRETFRRTATQALGKAPQEIL
                     LKNGETLFGSVFGLEGKYQVYTAQGIRVVDQDQVEEIRFE"
     gene            complement(11468..11908)
                     /locus_tag="PLG78_03035"
     CDS             complement(11468..11908)
                     /locus_tag="PLG78_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNE22112.1"
                     /translation="MLLFSPRTILAIWGLPLPASASQSETLTEGDLESFLNQHGNHVY
                     TYLCVMCRDEDAASEALQNAYMKFIEQVRRSRVRRDTAAQYLLTIAKNDHFSRVRKEG
                     ADLETICEKTGRSQATVYRLMEKALTILADACRRAGLHPEDAGL"
BASE COUNT         3107 a         3226 c         3021 g         2924 t
ORIGIN      
        1 acgactctct gatccgggaa ctggttctgg tgcgaatcaa gtcagacggc tcaaaaagaa
       61 atgagatttt tggaattgta gaagtgtttg gcggaaaggt cgcggaagta acggaagatt
      121 ctatcctcat tgaaatacac ggaagcggca gacaaattca cgccgccata gacatcctgg
      181 cccagtttgg tattatagag atggcgcgca ccggcgccat tgcccttgcg tatcgcacgg
      241 acactggcgc gggttaactc actgctttga cggagcggga atgtcaggac gatctgtctt
      301 ccgctttgcc agcatcaggt tagaatcacc tttgcgaaac ttatagccaa tcccgaacgg
      361 aagtggttta ggatgattct tagggagata catttcgtat agatctttct gatatttccc
      421 atcaaacaat ggaatgggtc tcgtgtaaga cccgtaaaac gtaagatcaa aactctgtgg
      481 cttgaagtac tgaagaggaa ttcctgaatc atcttgaagg atgagcttgc tttcgtccag
      541 gatgaaattg cgaatagttg taaaatcttt attatacatc aaataagaag cagccttgag
      601 atacgtggtt ggcgtaccca gactctgaat gtatttcagg aagtaaggct tctttgccag
      661 accctcttct gaaatatcaa tggagaaata atccatgtga gtgtattcag tggctcctgg
      721 tttttgaaac acaacgcgaa ttcctgaaac gtagtcctct gaggttgcag ccggttcagg
      781 ttttccggaa aacggatccg gcagagattc aatcttccca tcaggatgca ggcgaatgtt
      841 gtgcactgaa tgcactttgt ttcctgttcg cgctgcaaat acgagaagca cgggtgccac
      901 accggaaagc ttggttgctt tcaagtcgtt gaccatatgc agagtctgaa agaacgaata
      961 gtttagaatg ctgcgaagcg aaacctggag accggccaga ctctgattta catccgccgg
     1021 tttcattcct ttcacagaag gcggaggtcc tggaggttcg agaccaaaga agacgtactc
     1081 tgacccttgc ggaaagaaag tatatatgtt caggaaatca gcgccgctga aagggtagaa
     1141 gataaaccct tccgtttttc caagcacctc atcgcgccac ttacgctgcg gctcaagttg
     1201 agttttttcc acttctggcc agatctttgc gaagtacgcg cgctggcttt cggctgctgg
     1261 aaggccttct agatcagaga tagtgctgcc tgcatccggc tccatgcctg cgaggaagcg
     1321 cgcaatgtcg ttccattcgc gatgcgactc tgaattctca cgggctgatg ccggcgaatt
     1381 gccgcctttg cagagcaaca atgaaacggc gagtgccgcg acagaaatac gaaaataaaa
     1441 acgcatgagt ttatagaaaa aaactcgacc tcaagtcaat tataatttgc cgcgaaggtc
     1501 agggcgccga tgcttgtaaa gcgcgcgcac tttttgcgcc accgtctttc ccaaatgaac
     1561 aacacggaag ctctagcacg ccggcttgga ttctggagcg caagcatcat agtcgtggcc
     1621 aacatgattg gctcaggcat tttcggaaac accggataca tccaggcaca ggtaggttct
     1681 cccatcatgg tgatggcatt gtggactctc ggaggactca ttgccctggc gggagcgctc
     1741 tgttacgcag agttatccac cacgtatccg catgctggcg gagaatacgt ttacctaaag
     1801 catatctttg gcgggcttcc ctcatttctt actggatgga tttctcttct cgttggattc
     1861 gcagcgcctg cggcggcagc agcacttctc tccggcgtct acatgagtga tttcttgaaa
     1921 gtggtttatc cggacaccat cctttctcgc tttctgctgg atgaaaccaa tcgcaagatc
     1981 cttgcttctg cgttgatcat tctatttggt atttttcatt ccttcggggt caaacaaagt
     2041 ggccgcgttc aaaattttct gactggatcc aaaatcattg tcatcattct gtttgcagtc
     2101 ggcggcctca ctcttgctca cagaaacccg ggcattccca ttcatcaggc attttcaggc
     2161 gcagtcaaac cagagggact gggcgttggg ctcatgtttg ttatgttcgc ctacagcgga
     2221 tggaatgctg cgtcttatct cgcagaagaa atcaaacagc cggaaaagaa tctgcccagg
     2281 tctctcattg ctggaactct atttaccatc atcatctacc ttgtgatgaa ctacatttac
     2341 tatctggccg ttcccgcaac agaactggca gggaaaccgg atccggccgc tcaggcagcc
     2401 aaccatctgt ttggagagaa aacaaatcgc atcttccacg ttgcctttag cttcatgctc
     2461 ctttccactc tgtccgcctg tgttatgatc ggacccagag tgtattacgc catgtccaga
     2521 gacgggctct ttttcaaatt cgcagggcag ctctcgcaca cgggcgttcc tgttcgctcg
     2581 atcttcgtgc agtgctttat ttcgcttctg tacattttgc ttggaaccta cgaagacatc
     2641 cagacctaca tgggcttctc cctttcattg tttccagttt tgactgtgtt cggactcatt
     2701 ttgcacagaa aacgattccc gaaattagag agagcttaca aatctcctct cttcccattt
     2761 cttcccatca tgttcattct actgtccggg ctcattgttc taacgagtta cctggataga
     2821 ccgcgcgaag ggaacttcgc attgtctgtt gtgctggccg gagttcctat gtactatttg
     2881 tggatgcgtc ttcgcgccgg gaattccacg caccttctgg ctctgatcac cttacaggtg
     2941 ctatgcgttc ttgctgctgg cgttgcgtac catgtcctca agtacaatcc ggacgcactt
     3001 catatgagtc tggcagcact tggcgtggaa gtgatcagtg tggctgtgtt ctcttactgg
     3061 ctgcggagac aatatcagtc tctagctcgg tgaaaatttc agagagcccc gatccaagac
     3121 ccaccactag agcacgaggc gatttttcag gaagcgcaat ggatctattg tacagcttct
     3181 gaaacaccac gccaccattt gtagcagaag aagaaatcaa gaaaagttga acagatacct
     3241 ccgcgcgagg cgaagatcta aaatcacccg aaagattctg tatcactccc tctagaacaa
     3301 agtcttcgct cagagaggag gacgaatcca cgaccgtggc aaaagatcct gcctggaaca
     3361 gccagttgcg cgccgcttcc gaaaccatgg cccctgcatc cgtcaaatat tcgttgtaaa
     3421 agtctgtgtc atactgcaca tctccggttc tgtacaccag gcttcgagta tcaaagcgag
     3481 gcgaaacgcg aaatcgcctg actctcagga ttccaggctg tgcggcgacc ggaggacctt
     3541 tcctgggtat gtcgaggctg aagtattgtc gtgccggata ctggcgatcc gacacacaat
     3601 gagaactcag caaaagcaga aatgacaaga ccaggtattt caatgactga aactcgtgct
     3661 tttttccggt gccgttccaa acaaaagaag ggaagggtat ctcttcgccg ttccggtcaa
     3721 ttctttcata tcgcgcgaag cagtcttgag gttttccagc gtttcttcca tttcactttg
     3781 atttctggtc agaagctgat caatgcgaga catagtctga ttcaatttcg caagagaccc
     3841 gcctacgttt tctggaatgc tttttgtttc aggcgaagac aatagttcgc gaaaggaatt
     3901 atttgtctta cgcacttctc caagaagtgc caccgcttcc gcacgcagct ccggaatttt
     3961 cgcatcgtct acggcttttg tgccgctaat taaaaagcga tcgaggtcca cggcaagatt
     4021 ctccacattg gaatcctcaa gcttacgaaa gaaccggtct atgttcgcgg aaaggcgagt
     4081 gatggtgctc ggagcgctcg ggacatagac agatctggga gcccagacaa tgggcagtgg
     4141 tttgttttga tccggtggaa gataatccag ctcaagatat gcagttcctg tcagaccctg
     4201 tgacgtgagg cgaacgcgaa gaccgtcctg aatcattttc tgaagaatcg tgtcgtcctg
     4261 accgcgcata ccgagagggt ccgtaagtgc gattcgcaca agcacgtact taccatactt
     4321 gtagtagtca tcttcggata actccccgta ggtgtttcgc acaaagccaa tcgattccac
     4381 cgtgccgatc tgcactcctc tgtgcttcac cgcagaacca acatccaggc cctggacgga
     4441 ttcatcgaaa tacgtttcca tgcgaatggt ctttttgaag aaagagccgg ctccaagcaa
     4501 taccagacca acggacagta gcaggaatcc ggacataaca aacaaaccga tcttgtagta
     4561 acgggtcttg cttttcacgc ctcacccctt gagaaaaact gtttcactcg gggaacggtg
     4621 ctcgcggcaa gatccccggg ctttccgaca gcgataatcc ctcgcgcgga cttgtcgagc
     4681 atgatcacgc ggtccgcgat cttctggatg ctggagagtt catgcgatat gataacgaac
     4741 gtaatgccca gattacgaga caaaaaataa atcaggcgat ccagttcaaa cgaagtaaca
     4801 gggtcaagtc cggctgacgg ttcatccaga aatagaatct caggatccag cgccatcgcg
     4861 cgcgcaatgg acgcgcgctt caacatacct ccactgatgc gcgaagggaa gtgatgatca
     4921 taaccttcga gacccacaag cttgagtttc atgagtgcga gcaaatcctg agcttcctcg
     4981 ggtaagcccg aatactcttc caggggaagt cttacatttt caagcaatgt aagagagccc
     5041 agcaatgctc ctccctgata catcactccg aatctgcgaa ggatggcaga tcgatcatca
     5101 cctgtagcac ctacaagatc gcgatcacca aacacaacag aacctggatc tggttctaca
     5161 agaccaacca tgcttttgag cagagtactt ttgccgcaac cgctcccacc aatgataaac
     5221 agaatttccc cgcgtccgac ttcaaaattc acattttcca gaatcgtcgt gccatcgtac
     5281 ataacgcgaa ggtccgaaac gcgaatgaaa ggtggttcca ggttcgcttc catcaccatc
     5341 ccataaaata gaagatcacg gcaaagatcc cgtcaaaaac tgcgatcaaa atgattccgc
     5401 tgactacagc actggtcgtc gaagctccca ccgcactggc gccggccttt gtctcaagac
     5461 cacgatagca tccgatcgcc gcgatcagca cgccgaaaac cgttaccttg aaaagggctc
     5521 ccagaagatc ggaagtggcg gcggcggatt gtacctgctg cacgtaggtt acaaaaggaa
     5581 agccaagcga tagcatcaca accgcaccac ccgcgagtcc cgcgagttga aagaataaag
     5641 taaggagtgg tgcgacgatc aacgatgcga tgatgcgcgg aaccaccagg aatttcatgg
     5701 gatcgaatcc catcgttaca atcgcatcga tctcctcatt gattcgcatc gttccgattt
     5761 ccgcggcaaa cgcagaacca gatcgcccgg caaggatcac ggaagtcatg agcggtccta
     5821 gttcgcgaat catagaaaga ccaatcagat ttgcgacgaa tatctctgcg ccaaatcgac
     5881 gcatgggaat ggcagattga aacgcaagaa tcaacccgat caaaaaccca atcatcccaa
     5941 tcagaggaag tgccccgact ccagcgcttt cgcaggtgat gaatacatct ttccatcgga
     6001 tgcgcgaagg attgctaata gatttcatca gagccacaaa caactcgcca caaaaggcag
     6061 tgaattcctt cactttttcc cagagactga ccgtacgcct tccaatcgca tcaaagtgcc
     6121 acctgatctc ttcgtcttcc caggtttgtt ccaggattgc acgatcgaac agatgatata
     6181 gtttcctata cgattctgcc aggccaacta tttcaaaagg caggtcgagg gtagattgat
     6241 gcttctctag atgaatgagg agggcagccc ccgccgtgtc catagcgtcc acccggtgag
     6301 cgtccacaga gatcccgcgc acaccagtct tcaatttgtg cataatctct ggaaacatgc
     6361 ttccggcagt ctcgaaggtg agggggccgt cgaatgtgat gcgaagagaa tctccagaaa
     6421 cttcaaaacg aacagacatc gcagttcaat gcctggaaaa aaattcgaaa gccggagcta
     6481 gttgagaatc ccagaagcga tagtcatggc cgtagccagc tttcaaatgc acctcaacga
     6541 gtgtcttatc gcgtgcggac aaaagatctc cgaacatctg ggtttggtcc ggtggaacca
     6601 cacgatcgtc cagaccatgt gccagataaa taggagtctg ccattctgaa gcgcgtcggg
     6661 cagggttatc gcggcctttc catcgatccg gaaacagtgc atacggtccc cagattgcgg
     6721 cattgattgc ttctcctggc attctctcct gggcatagtc tccagagaaa gcagcacccg
     6781 cagcaaatag tttaggattc tcaagtatgg tcatagccac accacgtcct ccggtggaca
     6841 gacccagaac gaagttcctt tgcccgggca tcaagagcga ataacgtttc tgcatctccg
     6901 gaatgagagt atcatgaatg aactgagagc ccggggtctt gttccatcgc agagttgttt
     6961 cagggtagta agatgtctcg tagagagttt tgagcatatc cggcaaaatc agaatgagat
     7021 gatgcttgcg tgcctgttcc tcaatgcttg agttttttct ccacgatcct ctgtcaaaat
     7081 tccagcctgg caaaaccaga accgttccca cgggtgcgtc tggaacaaaa acatcgcaag
     7141 gcactccgcc cacatctacg gtggaaagac cggcagcagg aggattcaaa ggtgcgctcc
     7201 tgggagcgtt gcaacaaagg aagcttaaga aaacaaggag tgcctgtcgc atgccacgac
     7261 agtccagggg catgcctgca aggcatcaag aatttgcatg catccagctt gttcaaaaca
     7321 ctccggttcc gataacttgc attatgtctc ataccgggtt ctgagattct ttccctggtt
     7381 ggcgcggccg caaacaaaaa cagaaaatgt gccagggaca caatagcgaa atcgtagttt
     7441 tatgattgcg cacaaccagg gggctgacga gaagaagcgt gctacgagtt cgaaccagcg
     7501 tccgccccag tcagatagcc ggcctggggc tgtttgcggc ggaagacatt tccaaagatc
     7561 agatcatcta cgaatggact gcgaagtttg aacaaacctt cactgcagaa gagtacgaga
     7621 aacttggccc gctccagaaa gaatttcttc gccactattc tttcaaacga tcgggaatcc
     7681 gtgttgtgtc cctcgatgat gatcgatttg ccaatcacag tgacactccc aacacatatg
     7741 agaaaggtgc agacactcat gcgctgcgcg atattgcagc cggtgaagaa attacatgca
     7801 actactacga ttttgacgaa agcgcggatg agaaactggc tggaattcag cgttcgtaaa
     7861 acaagaagac tctcgcacat ccgcgcaaga gtcttgattt tgatttagca aagcgagtca
     7921 cctcgccttg caaacttcat tcataaactc agttcttacg catgcagtag gtgagcgcta
     7981 cgttcttggg acgtgtttca tttgcagtgc gaggtgtgcc attcactccg tctgacattg
     8041 ggccacctgt aggtccatcc ttggaaagct cctgaagccc tcccgctggc aagaagttgt
     8101 tgggatttcc tagccagact ctggattgat gcacgtgacc ctggaatgcg tcggcctgca
     8161 gcgccccaat gacgcgactt ccatctggat ccatgccggc cggaccggtt cccacatcat
     8221 cgcgaccgcg aatgaactga ccgcgcagat ctggagttcc atttgtaccg tctgctggcg
     8281 ccaaccatcc gggcaactgg tcaggtagaa agccatcacg gcgccttctg gagcggcaat
     8341 tgcaaagttc tgattgatct gcgctgcact gatcacctga ccaggcgaaa atacgttgat
     8401 ggcggctgcc gcggcaaaga tacttgtggt caatagacca actccaaggc caacgccttt
     8461 gaaaaatcca ctcttaatgt attccatgat cttgttagtt gttttcattt ttcttttccc
     8521 attctttaaa ttttcatact aaattgcaaa tcgtttctca cgttagttga acacgcaaga
     8581 cgtatcaaaa tgccccacgt caaactgaca gctggctccg cttccgtgta cgagcacgtt
     8641 gtaagtcacg gagaaaccgc ctccgagggt cgctgtgatt gtgccggtag tgtccgcagg
     8701 cagaggtgct gcagtgaact gtccgtagtt ggcaggacca ccgaacgaac caataccgcc
     8761 ggcaacgctg aagacaggct gcacgaacat gaagttgtcg aacgaatcat atccggctgc
     8821 atccagctgc ttgactgtac ccgcatttac gttgaacgga tcagaaagca ctgcatcgtt
     8881 gtagagaact tcaatgcgac tgagtactcc tggatagacg ttaatcggca ctgagagatt
     8941 gagagttcca tacgtcgctt ctagaaacga tgatccaatc ttctgagcag tgtatctggc
     9001 actgagccac gtggggcgag cagtcgtgcc tttggcaccg gcgaatgtca atgtctctgc
     9061 tgctactgga tagtaggttg cggaagcatt gcctctcacc tgcaccgtca gatctctcga
     9121 gaaacccgcg ctgatctgcg aaacgggagt ctgaaaacga atgggagctg cggcaaacac
     9181 agtatcgcct ggcacagtcc cgagagccac aggagcggtc gccggattcg tgcggcctgc
     9241 attgagcgta tcaaaggcct ggatataata ctgaaggcca gccgaagtta cgtctgcacc
     9301 tggaactttc gcagtgtaga attgacccga accgtcgttt gaatccggaa tcatcacaac
     9361 actcttaaaa gcagcgccgg atccagtggt gcgataaaag aagcgcacgg ttgcgatccc
     9421 tgatccaccg tcccagtctg tcactgtact tcgaaccgtg accggctggc ccgccatcac
     9481 ccgaccggga aagaactgcg ctccaccgat cgtgggtgcg ttgttgcctg ccgagagcaa
     9541 ttgtgcagtc agaatgtaag atgagaagtg atagataggt gcgctcacgc aatgactcgc
     9601 ctggtctacc tctcctccca tgctaacgaa ctgacccgat tcttcatcga agtactgaat
     9661 ctgcatcgtc ttctcctgca atccgcgctc attgacattg cgagcgtcgt agcagatttg
     9721 cagggtggct ggcttgctga actgcaggtt ctccggccca aatttatagg ccgcctgcaa
     9781 tggctgaatt gtatccgtcc ctgcgggcac gtcgattggc tcgtaggtga tcgtagtttc
     9841 ttcttcgagc gccccctggg gtacagtgag actcatagtc ggggcctgga tcgtggctcc
     9901 gctggctggc ctgacgattg ggcttcctgc tgcactgagc atcagaagtc gactcaagct
     9961 ggaatcgcct ttggatttct ggcagcccag agcaagcaag cagagcaagc ccaggcacca
    10021 tttgaatttt ggtttcattc cttaaacctc cttatcttat ttgagccggg agtttaggat
    10081 cgccggttca aatggagtag ggagaaaggc ctggatgatt ctcaaaaaaa atcggagcga
    10141 atagaaaaaa acaagaaacg attcgatttc gatcactcga atcgaatttc ttccacttga
    10201 tcctgatcta cgactcgaat gccctgggca gtgtagacct ggtattttcc ttccaggcca
    10261 aagaccgaac caaacagggt ctctccgttc ttgagaagaa tttcctgcgg cgccttgccc
    10321 agagcctggg tggccgtccg cctgaatgtc tcacgatccg gggcaggatt ttgcttcaac
    10381 aaggcttcat aacgaacttt acgttctgct tctgaaggtt gttccagaga ctgccgcatg
    10441 gctgatagat cctctgctgg aactacagtg acttccggcg gctggccaaa cgccgtttcc
    10501 acaagacgaa ggttctcttc tcgctctctg ggacctggaa aattgctttc aatgatcttc
    10561 ttcgcagtct ccacctcaga atcagaaact ttcaagcggc ctcgcaacgc ggaaatgcga
    10621 ggatctgaaa gtatctttcg caaatgaggg atcgccccga gtcttctctc aaaatccatt
    10681 ccggaaacca ctgcctgcga acccgcgggc acggaagcgg aagctttcaa aaacacatcc
    10741 cggctctggg aaaggatttc gcttcgctgc agcaaattct ccggtaaatc ctccagatcg
    10801 gaccagcggc gacgaatgga aacggtgccc tcgaatgtag acagcgaggt gtcagaagat
    10861 cgactaacgt ccactgagaa tacagtacca gtcacgcggg tctgtgcatt gtgagtagaa
    10921 atcagaagtg cccgagccgg actgtctttc ctgctgagct ggcttaacac aattccgcga
    10981 accaggcgcg cttccaccag atctgcctgg agcaccggaa gagtcagttc agaattcgcc
    11041 cggactcgaa gaccaatttc tgttttttta tccgcagatc caagaaccag ggaggaggcg
    11101 tcccctaccg ttattcgctc atgttcacca agcgttccag ttttgacagg ctggccttct
    11161 tttctggtat caccgtcaga aaacacaatc ctcataggag ggacgtccac gcgaaagact
    11221 ccgaaataaa ctacaagtgc agccgcaatc gcgccggaaa aagataccgc tgccgccaca
    11281 ggccatcgaa tgacttttgt tttaaaactg gccctgctct ctttctggcc atgcatggcc
    11341 gcgagtttag atcgcaggcc ttcgggcatg gcgcgaaggt ccattcgcaa ggcgcgaagc
    11401 tcttcaggcc acgatgcagg tgcttgctcg aggaggcggc gagccagttc ttcgcgttgt
    11461 gaatcgttca taacccggca tcctcaggat gaagtccagc tcgcctgcaa gcgtctgcaa
    11521 gaattgtaag ggccttctcc atcaatcgat agaccgttgc ctgagagcga cctgtctttt
    11581 cacagatcgt ttctagatcc gctccctctt tgcgtactct tgaaaagtga tcattctttg
    11641 cgattgtgag tagatattgc gcagctgtgt cccgtcttac tcttgatcta cgcacctgct
    11701 caatgaattt catataagca ttctgcaacg cctctgacgc tgcatcctcg tcccggcaca
    11761 tcacacagag atacgtatac acatgatttc catgctgatt caagaaactc tcaaggtccc
    11821 cctcagtgag cgtttcagac tgcgatgctg atgcgggaag aggcaggccc cagatggcaa
    11881 gtatggtccg aggggaaaac aggagcatga ttagaatacg atctcagcaa agaagattcg
    11941 caaagaaaat gctcgagaat gcgattccag aaattttttc atctggttca gcgactgaag
    12001 gaacaactat cgaagctggc ggcgtcgaat ttgcatggag gcgcaccggt gatcaggatc
    12061 atgatatatc cgcccagccc cctgggtcct ttcatatcga agctattgtt caggcgcgaa
    12121 gcgatgcaac tgcccgtaat gagcagaata cagatcaaac cagaacgtag cacgtttgtt
    12181 ttcataaata gtaccgcacc gcagccgtaa tcgacccggg ccccgagcta atgcacaaca
    12241 ctggaacctg ctcgcgtgga gcggaaccca accagcgc
//
LOCUS       DAOQJL010000040        12226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2586_length_12226_cov_4.783666, whole genome shotgun sequence.
ACCESSION   DAOQJL010000040 DAOQJL010000000
VERSION     DAOQJL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2586_length_12226_cov_4.783666"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLG78_03040"
     CDS             <1..1201
                     /locus_tag="PLG78_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE22113.1"
                     /translation="FAETRKHPSKPIGSIHPGVVHRGNLPEQTVRISGLKAVMLGGNV
                     DGIEGATTVSYRGYLKRVAAVLRARGVQVTEIYSPTKKETIAAAISGAHFIFYAGHGI
                     GSSEPPRYQGDITPAGMLVVDEVWSNAEDVAQWKPAPGAIVFFLGACFTAGNSGDDIG
                     KIQDAEAKRRIGVYSAPFFLRNRFGAYYAAWSDSAAQDVMAELFAGRTLGQAYDPDGN
                     MSGVFKSQHPNVSSSELWYHRSNYDNRGYAFDYAFVGKPGMTLEQLFKDTSQTTTTTT
                     TTPTTNENKIDPEEAKRKGTELIRAIYDGQDASAIRLINEGADTNVRYSQWTPLMLAV
                     YYDRPSVVRALISVRADLNAMMDTWTALSLSDAYGRTEISGLLRAAGAKTERAMPGTR
                     PLAPPRR"
     gene            complement(1219..2181)
                     /locus_tag="PLG78_03045"
     CDS             complement(1219..2181)
                     /locus_tag="PLG78_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNE22114.1"
                     /translation="MRFFLLFLAAISLHAEPLPELKTPVTDLTGTLSKEQISNLENRI
                     LGLEKEKGSQIAIAIVPTTSAENIEQYSIRLATAWKIGRKGVDDGVLLVVAKQDRKLR
                     IEVGYGLEGAIPDAYAKQIIDMTIVPRLREGKFYEGISDGLDRIISLIKTESLPPAQK
                     EKAIPRMSNTEWMLAVLIHGGFWLRPALLLLGVILFIWKRDLKGLVIPLGIWLAWILF
                     WSLRVQAFFFSGLISAAVYSLVYYLFLNRFRSSTGASSSSSGWFSGTSSRSSTYRPTN
                     SSYRSSSSSSSYRSSSSSSSSSSSRSSYSGSGGSFGGGGASGSW"
     gene            2271..3521
                     /gene="nuoF"
                     /locus_tag="PLG78_03050"
     CDS             2271..3521
                     /gene="nuoF"
                     /locus_tag="PLG78_03050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE22115.1"
                     /translation="MAVFEPVLTKHIYESGSETMDHYLARGGYGTAREWIGKEPAEIQ
                     KIVNDSGLRGRGGAGFPTGRKWSLLARGTDKPVYLAVNGDESEPGTFKDRYLLERDPH
                     QVLEGIILTSYAIGSHHAFLYMRGEFIKGYEMMRQALEDAKKKGYIGSNIFGKGYDLK
                     VTLVRGAGAYICGEETGMLTSIEGNRGYPKVKPPFPAVAGLFGCPTIINNVETICDVV
                     HVVKNGAAWHRQWGTEQSPGFKLFCVSGHVNKPGLQEVPLGTPLTEVIANAGGVLPGK
                     KIKAVIPGGSSTPLLTGDKIEKANMDYESIAALGSFLGSGAITVLGDDVDMVRSVFNL
                     MRFYHHESCGQCTPCREGTGWLEKLVHRVYAGHAQKSDLDLINDVCNNMRGRTICALS
                     DAAVMPMTSYLKEFRAEFEARIPT"
     gene            complement(3532..3870)
                     /locus_tag="PLG78_03055"
     CDS             complement(3532..3870)
                     /locus_tag="PLG78_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22116.1"
                     /translation="MKNQLSRTEGRGELFMHDVSGRYRFTHMTESGQTVTLMLLLTQV
                     GTDIRGKLRSIFASAGDDQLYYTEEHDVRGNWNEGPIALLLPEGQITVESAPGGLNVT
                     RDQTEFWKSI"
     gene            complement(3867..4007)
                     /locus_tag="PLG78_03060"
     CDS             complement(3867..4007)
                     /locus_tag="PLG78_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNE22117.1"
                     /translation="MLLSLGEEAVQEIIKEHGLVEVFCEFCRERYEFRPEQIGTLFGH
                     GK"
     gene            complement(3992..4720)
                     /locus_tag="PLG78_03065"
     CDS             complement(3992..4720)
                     /locus_tag="PLG78_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNE22118.1"
                     /translation="MFDPSQNYFVRGLLPAWNYRLVLGSITPAAIEAGRRHSASNQMT
                     CLFGEAMLGAFFLECAGSKSDAIKTCLHLEGTGPAQRIIAFASIEGRVRAHAGVPQAQ
                     WNGSLEEGKGPGFVKVSRWFSDSNIYTSSVEMRLAPIAKNLEDYLTRSDQVQAFVKIQ
                     TIFGPDSEPERISGCMIQALPGASATDTDHALGWLGAAWPDLGREIDYKDGILSKSPF
                     RTLGAGEFQFYCDLWTGYPICCFL"
     gene            complement(4768..5838)
                     /locus_tag="PLG78_03070"
     CDS             complement(4768..5838)
                     /locus_tag="PLG78_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22119.1"
                     /translation="MRFEFREAKPEDNSEICEIFRIPMGGSLALSLERDPDYFAGARV
                     QNEKPRVFVGVDHEKHCLTGSFSVGERRVYLNGKPEWIPYFCDLRIRDEYRSGTLLAR
                     GYKFVKEEVLAGRYAQTIIVADNQEAINSLTGSRAGLPTYVPFGAYVTRAIPGNAASK
                     MKYDASISIRKAQMADLGLMKSLCEEAGRQKQFYPCYDFEFSSPYFRDLSFDQYYIAY
                     RNGSPVGMTGVWNQKSFKKTRICAYGRGLNLARPLYNAWMKLKGGISLPEPGSILPYS
                     YLHTVNTKNNDPQILRALVREIANDRDDAEYFLLGLDARDRLGDAFRGLSVREFHGFH
                     YLVDAGATLPEPSLFYLEAARI"
     gene            complement(5828..7363)
                     /locus_tag="PLG78_03075"
     CDS             complement(5828..7363)
                     /locus_tag="PLG78_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNE22120.1"
                     /translation="MNLAGATLRLWLASGHAEYSRFSAHAADPMKDQTKNLGRIFRLM
                     RSTKFASEHHLGDLDETSTPERLLGALRQVPIRSATEYEPWLADEAETAHILCAKTPD
                     FFEETSGSVRPKLIPYTRELRREMDIAIGVWMNALYAQDKSIFQGPLYFSLSPTLKAR
                     KLTARGIPIGANDEDYLGAATRILLRYALTGRPASFRSPEDFFRKTLEQLIAAKTLSM
                     VSVWSPTFLISLDARLREMWADLKKPSSVPARFETWDEIWPNLKMISMWTDASAAAFV
                     PQVRSITGNIPVQGKGLLATEGITSIPILHDMDPVIAYRSHFFEFKNERGIFRLDELQ
                     AGESYEVILTTGGGLIRYATGDIVTVTGFMRRVPTIRFKARNEFSDLVGEKISEEAGL
                     AAIHRALKVCHGICGIFLRHADPGAKMGAAGYELLLFVESVPENAPSVASAATQAATE
                     ILEQNPYYTLAIQTGQLRNLRVRFLGRDALEKMQKKLAAQAKREGTWKWPAFTSEAHP
                     DEI"
     gene            complement(7360..8025)
                     /locus_tag="PLG78_03080"
     CDS             complement(7360..8025)
                     /locus_tag="PLG78_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22121.1"
                     /translation="MFQLLEENYSSVSREIFYEDLGSKDFVGLLMDHANIIQGFTTIA
                     LRTRHPDYNVLFSGDTIITPKHWGSQELVRGFCNAAGSFLATSTKPLYWFLISKGHRT
                     YLYLPLFTRKFFPDPNSQNEDLAAIAKTESMLMFPQYYDQEQGLLVFPESRGELRPEL
                     IAATLFRETNAYIRFFRERNPGFVRGNELVCITELSPSNLKGIAKRWLVRGMSSNVIQ
                     AAV"
     gene            complement(8082..8660)
                     /locus_tag="PLG78_03085"
     CDS             complement(8082..8660)
                     /locus_tag="PLG78_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22122.1"
                     /translation="MMAAMLLIFQAAAPLFYLKQKSGKAVGGPISMVKSFWLIFAITL
                     WILLPSILSFTAPGELRICFQLLAVSMILRGVVELYLCYGTIRWKTEYGIAHDSLQIG
                     ICITLLTTRSLPAVHALIILLTILSLITEMYFVHRFRISTSGPAQGVYFVPETDKRTN
                     RLTLLIFAPQYMAFWTLVFVLEYPLIQARIGL"
     gene            complement(8674..9735)
                     /locus_tag="PLG78_03090"
     CDS             complement(8674..9735)
                     /locus_tag="PLG78_03090"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNE22123.1"
                     /translation="MVWPGNSRNRSASWTLYDPGAEMIEAFRPIENYWYIACFSRELK
                     SKPVSRTILGKPLVLFRTGEKPACLEDRCPHRNIALSLGKVKKDGLQCSYHGWTFGAD
                     GKCLRIPAESESGARPAARSLPAVESQGFIWVFLGDKVPGPVPEFPMFSAPRWQNWFM
                     EREFDGSAFQCIENFLDVPHTVFVHSGLFRTREGSDTTLSIVRNNQSVTAEFLNERQV
                     DGLVGKLLVPAGSQAKHTDAFLMPSTTRVDYYFSEKRHFIVMSQCTPVDEYRTRVYTY
                     MAFRFDPIAPIVRLIYAPLSSMILNQDVRVIKKQSDNIKRFGREAFYFHQTDAIAREI
                     REMMSGRKARSSHKKRIRA"
     gene            complement(9666..>12226)
                     /locus_tag="PLG78_03095"
     CDS             complement(9666..>12226)
                     /locus_tag="PLG78_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNE22124.1"
                     /translation="IIVALSAGALLDVIFCGLLKGKWIFPLSAVITTLSLTIILSFSS
                     GYHFIWLPVLIAIASKYIFTLNGRHIYNPSLFAIVFSLIFADGWISIAPAYQWTGSAE
                     TAWLMSITILTAGVTIFGYRGSRKWLALSFLTFFAIQLLIRSYIIRYHLPASALFIGT
                     ITSPSFYLFTFFMITDPATSPPDKRTQILVGFFLAIFDLAYHLKFSLFTFFYAAFTLA
                     TIRYVVAAWKTRAWSGALRSHIPAAILTFFFAIPAFAMYESGQSNVRGSAPPGLHFTR
                     IASSQSGIGYEKSNILEQVDPRVQHIAKWLLSVGDSVASADVDLDGKPDLFFTQSLKS
                     TEYRGKLYLNQGNFQFKKIVIPDLERYLNDPAKFGLPAFAFFFDYDNDGDQDLFVGFG
                     YGRSHMFENRIKQEGIVRFREIETQATKDHTIALAANAFDFNRDGRLDVLVANTLPVY
                     LPDYLPEKIPLNIFKLPEPRYEGDRRMLHFMHHSWHNANNGGKNELYLGTKGPDPFSR
                     TDVGLDETHWSLSVGTADLNRDGFTDIYIANDFGRDDCYINDGGKRFRRQQGEFFEDL
                     GLDTYKGMNASMGDIDGNGADDVYVSNVHHPMQAEGSLLWMNYTASGAQNLSMKDRAS
                     QRGALNPHRFGWGAAMADLDLDGKLDIVQANGMVSNKWDKRYATCLDYWYLNEKLARS
                     IPEIHTYADNWADLRGACIYPDEADRIYINQGSRFSDFADDVGVTHRANTRGVAAVDL
                     DNDGDLDLVFTDQFGEPIIYRNDINKPSWAGLILHGNGRTCNRDAVGSTVELKSPSIS
                     QHREVRLVNGFSAQSDTRLLFAVTGSARAVIQWCGQEIQEIDLPAGRYTTLEQR"
BASE COUNT         3047 a         3025 c         3094 g         3060 t
ORIGIN      
        1 gtttgcggag actcgcaagc atccgagcaa acccatcgga tcgattcatc caggtgtggt
       61 tcatcgcggc aatcttccgg aacaaacggt tcggatttcc gggctaaagg ctgtgatgct
      121 cggtgggaat gtggatggaa ttgagggcgc gaccacggtg agctatcgcg gatatctcaa
      181 gcgtgtggct gcagttcttc gcgctcgcgg agtgcaggtg acagagattt acagtccaac
      241 aaaaaaggaa acgatcgcgg ccgcgatttc cggggcacat tttatttttt acgcaggtca
      301 tggaatcgga tcgagcgaac cacctcgtta tcaaggagac atcactcccg ccggaatgct
      361 ggtcgtagac gaagtatgga gcaacgcgga ggacgtagca caatggaagc ccgccccagg
      421 agcgattgta ttctttctgg gcgcttgttt tacagccgga aattcaggcg atgatattgg
      481 aaagattcag gacgcggaag caaagcgcag gatcggagtg tattctgctc cattctttct
      541 acgcaaccgc tttggcgcct actatgcagc gtggtcagat tcagcggccc aggatgtgat
      601 ggctgaactg tttgctggcc gcactctcgg acaggcatac gatccagacg gaaacatgtc
      661 aggagtattt aagtctcagc atccgaatgt gtcgagcagc gaattgtggt atcatcggtc
      721 caactacgat aaccgtggtt atgcgtttga ctatgcattt gttgggaagc cgggcatgac
      781 tctggagcag ttgttcaagg atacgtcgca aactacaaca accacaacaa cgactccgac
      841 cacgaatgaa aacaagattg atccagaaga agcgaagcgc aaaggtacag aactcattcg
      901 cgccatctac gatggtcagg acgcaagcgc gattcgcctg atcaacgaag gggcggatac
      961 aaatgttcga tacagtcagt ggacgccctt gatgcttgca gtgtactacg atcgcccttc
     1021 tgtagtgcgc gcactcattt ctgttcgcgc agatttaaat gcgatgatgg atacgtggac
     1081 cgctctttct ctgtcggacg cgtatggaag aactgagatt tcaggtttgc ttcgcgcagc
     1141 cggtgcaaag actgagcgcg cgatgccagg aactcgtcca ctggcgccac ccagacgttg
     1201 aatgtgaatc gggcatcttc accagctgcc cgatgctcct ccgcccccaa agcttccacc
     1261 gcttccgctg taactggaac gcgaagaact gcttgagcta gagctagagc tagaacggta
     1321 gctgcttgaa ctggaactgg atctgtaact gctgttagtt ggtctgtatg tagagctgcg
     1381 gctggatgtt ccgctgaacc atccagaaga tgaagaagag gcgcctgtgg aggagcgaaa
     1441 acggttgaga aataagtaat agacgagaga gtagacagcg gcggaaatta agccggaaaa
     1501 aaagaacgcc tgcacgcgca gactccagaa cagaatccag gcaagccaga tgccgagcgg
     1561 gatcactagc cctttcaaat cacgcttcca gataaacaaa atcacgccga gtagaagcaa
     1621 cgctggtcgc agccagaatc cgccgtgaat caagaccgca agcatccatt ctgtattcga
     1681 catgcgcgga attgcttttt ctttttgcgc tggcggcaaa gactccgttt tgatcagcga
     1741 aatgattcga tcgagtccgt cgctgatgcc ctcatagaat tttccttcgc ggaggcgcgg
     1801 cacaatcgtc atgtctatga tttgtttcgc gtatgcatcc ggaatggcac cttccagtcc
     1861 gtagcccact tctatgcgca gtttacgatc ctgtttggct acaaccagca gcactccatc
     1921 gtctacaccc ttcctgccga ttttccatgc agtggccagc cggatcgaat actgttctat
     1981 gttttccgcg gatgtggtcg gaacaattgc aatggcgatc tggcttccct tttccttttc
     2041 caggccgaga atcctgtttt ccaggtttga gatttgctcc ttcgaaagag tgcccgtaag
     2101 atctgttacg ggcgttttca gctccggaag aggttctgcg tgcagagaaa ttgcagctaa
     2161 aaacaggagg aagaaacgca cgagcattgg aggcatattg agaacagcct gtccatcatt
     2221 tcgcaggttt ctgcttgcac tccaggggcg gcccttttcc ctcctgaaac atggctgtat
     2281 tcgaaccggt tctgacaaag cacatctacg aatccgggtc cgagacaatg gatcattatc
     2341 tggcacgggg cggttacggc actgcgcgtg aatggattgg aaaagagccg gctgagattc
     2401 aaaagattgt aaacgactca ggtcttcgcg gacgtggtgg ggctggattt ccaactggaa
     2461 gaaagtggag cttacttgcg cgtggcacag acaagccggt ttacctcgca gtcaatggag
     2521 acgaatcaga gcccgggact ttcaaggata gatatctcct ggaaagggat ccacatcaag
     2581 tgctcgaagg aattattcta acgtcatacg ccattggttc gcatcatgcg tttctctaca
     2641 tgcgcggaga atttatcaag ggctatgaaa tgatgcgtca ggctctggaa gacgctaaga
     2701 agaaaggata cattggaagc aatatctttg gaaaaggata cgatctaaaa gtcacgctgg
     2761 ttcgtggtgc gggtgcttac atttgtggag aagaaacggg aatgctcact tccattgaag
     2821 ggaatcgcgg gtatccaaaa gtgaagccgc cgtttcctgc tgtggctggt ttgtttggat
     2881 gccctaccat catcaacaat gttgaaacta tttgtgatgt ggttcacgtt gtgaaaaacg
     2941 gagccgcgtg gcacaggcaa tggggaacgg aacagagccc agggttcaag ctcttttgtg
     3001 tgtccggtca cgtgaacaaa ccaggactcc aggaagtgcc tctgggaaca ccactgacag
     3061 aagtaattgc gaatgcaggc ggagtgctgc cggggaagaa aatcaaagct gtgattcctg
     3121 gtggatcgag tactccgctt ctaaccggag acaagatcga aaaagcaaac atggactatg
     3181 aaagcattgc agcgcttgga tcattccttg gatcaggagc gatcacggtt ctgggcgatg
     3241 acgtggatat ggtccggtcc gttttcaatt tgatgcgatt ctatcatcac gaatcctgcg
     3301 gacagtgtac tccatgcaga gaaggaacag gctggctgga aaaactggtt catcgtgtat
     3361 atgctggtca tgcgcaaaag tcagacctcg atttgatcaa cgacgtgtgc aacaatatgc
     3421 gtgggagaac catctgcgct ctttctgacg ccgcagtcat gcctatgacc agttatctca
     3481 aagagtttcg tgcggagttt gaagcacgca ttcctacgta agggctgcgc gctagatgga
     3541 cttccagaat tccgtctgat cccgtgtaac gttcagtcca cctggagcag actccactgt
     3601 aatctgcccc tccggtagca aaagggctat tggcccttca ttccagttgc cgcgtacgtc
     3661 atgctcttct gtgtaataga gctgatcatc acctgcagag gcaaaaatag aacgaagctt
     3721 gccgcgaatg tctgtcccca cctgtgtcaa aagaagcatg agtgtcaccg tctggccgga
     3781 ttcggtcatg tgcgtgaaac gatatcttcc gctcacatcg tgcatgaaca attctccccg
     3841 gccttcagtc ctggacaatt gatttttcac ttcccgtggc cgaataacgt tccaatctgc
     3901 tccgggcgaa attcgtatcg ttcgcgacag aactcacaaa atacttccac cagtccatgt
     3961 tctttgatga tttcctgaac cgcttcttcg cctagagaaa gcaacatatt ggataccctg
     4021 tccaaagatc gcagtagaat tgaaattccc cggcacctag agtgcgaaat ggagatttac
     4081 ttagaattcc atctttgtaa tctatctctc gacccagatc cggccatgca gcaccgagcc
     4141 agccaagtgc atgatccgta tccgtcgcag aagccccggg caaagcctgg atcatgcaac
     4201 ctgagattct ttctggttca gagtctggcc caaagatcgt ttgtattttc acgaatgcct
     4261 gaacctgatc cgagcgagtg agatagtcct caagattctt cgcgatgggg gcaaggcgca
     4321 tttctacgct cgatgtgtat atattgctat ctgagaacca gcgactgacc tttacgaatc
     4381 ccggcccttt tccttcctcg agggagccat tccattgtgc ctgaggaact ccagcgtgtg
     4441 cgcgaacgcg tccttcaatg gatgcaaaag caatgattct ttgtgcaggt ccggttcctt
     4501 ctagatgaag gcatgtttta atggcgtctg atttggatcc cgcacactca agaaagaacg
     4561 caccgagcat tgcttctcca aacaggcatg tcatctgatt ggatgcagaa tgtctgcggc
     4621 ctgcttctat tgctgccggc gtaatggacc cgagcacaag tcgataattc caggcaggaa
     4681 gaagtccgcg tacgaaataa ttctgggagg gatcgaacac gtgcctttgt aatgcgccac
     4741 cctcccgatt caatcaagaa ttgatttcta gattcgcgcg gcttccaaat aaaagaggga
     4801 aggttctgga agtgtggcac cggcatccac tagatagtga aaaccgtgga attcgcgtac
     4861 gctcaagccc ctgaaagcat ctcccagtct gtctcgcgca tccagaccaa gtagaaagta
     4921 ttctgcgtcg tcccggtcat tcgcaatttc gcgcacgagc gctcttagaa tttgtggatc
     4981 attattcttc gtatttacag tatgcaggta ggaataaggc agaatagaac caggttcggg
     5041 aagagagatt ccccccttga gtttcatcca tgcgttgtaa agaggccttg caagattcaa
     5101 tccgcgtccg taggcgcaaa tcctggtttt cttaaacgac ttctggttcc agactcccgt
     5161 cattcccacc ggtgatccat ttctatatgc tatgtagtac tgatcaaaag aaagatctct
     5221 gaaataaggt gaactaaatt caaagtcata acagggatag aactgtttct gtctgccggc
     5281 ctcttcgcac aacgatttca tgaggcctaa atctgccatt tgtgctttcc gaatggaaat
     5341 actggcgtca tatttcattt ttgaagctgc gtttcccggg atcgctcgcg taacatatgc
     5401 gccaaatgga acatacgtcg gaagccccgc tctacttccg gtgagagaat tgattgcttc
     5461 ttgattgtca gccacgatga tggtttgcgc ataacgtcca gccaggactt cttccttgac
     5521 gaatttgtat cctctcgcaa gcaaagtgcc gcttcgatat tcgtctcgaa ttctgaggtc
     5581 gcaaaagtac ggaatccatt ctggttttcc gttgaggtaa acacggcgtt ctcccacgct
     5641 gaagcttcct gtgaggcagt gcttctcatg gtctacgcca acgaagacac gcggcttttc
     5701 gttttgaacg cgcgcgccag caaagtagtc cgggtctctt tccagtgaaa gtgccagaga
     5761 tccgcccatc gggatgcgaa agatttcgca gatttccgaa ttgtcttccg gttttgcttc
     5821 tctaaactca aatctcatct ggatgtgctt cacttgtgaa cgcgggccat ttccaagttc
     5881 cttctctttt cgcctgtgcc gccagcttct tttgcatttt ttctaacgcg tcgcgaccca
     5941 gaaagcgtac acgcaagttt cgcagctggc cggtctggat ggcaagggtg tagtaggggt
     6001 tttgctcgag aatttccgtg gcagcctgcg ttgctgcact cgctacggat ggcgcgtttt
     6061 cagggacgct ctccacaaaa agaagtaatt cgtatccggc cgctcccatc ttcgcgccag
     6121 ggtctgcgtg gcgcaaaaaa attccgcaaa ttccatggca gaccttgagc gcccgatgaa
     6181 tcgccgcgag gccagcttct tcggagatct tttctcccac aagatctgaa aattcatttc
     6241 tagctttgaa acgaatcgta ggcacacgac gcatgaatcc agtgacagtc actatatcgc
     6301 ctgttgcata tcgaatcagc ccgcctcctg tagtgagtat gacttcgtat gattcaccgg
     6361 cctggagttc atccagacga aaaatgccgc gttcattctt gaattcaaaa aagtgagaac
     6421 gataggcgat caccggatcc atgtcatgca gaatcgggat cgatgtgatt ccctctgtag
     6481 ccagaagtcc ttttccttgc acaggaatat tgccggtgat ggagcgaacc tgcggcacaa
     6541 atgcagccgc ggaagcatct gtccacatgc tgatcatttt tagattgggc catatttcat
     6601 cccaggtttc aaatcgtgcc ggaacagatg atggtttctt caaatctgcc cacatctctc
     6661 gtagacgcgc atcgagagat atgaggaagg ttggactcca cacagacacc atgctcaggg
     6721 tctttgctgc aatcagctgt tcaagggtct ttcgaaagaa atcctccggc gagcggaaag
     6781 aagcgggcct tcccgtgagc gcataacgta gcaaaatgcg cgtggcagct cccagatagt
     6841 cctcgtcgtt tgcgccgata ggaattcctc tggcggtcaa cttcctcgcc tttaaagtgg
     6901 gcgagagaga aaaatacagg ggcccctgga agatcgactt gtcctgcgca tagagtgcat
     6961 tcatccacac cccaatggct atgtccattt cccgtcggag ttcgcgcgta tatgggatga
     7021 gcttgggccg cacggagcct gatgtttctt caaagaagtc gggcgtcttt gcgcagagga
     7081 tgtgtgctgt ttctgcctcg tcggcaagcc agggttcgta ttctgtcgca cttcgaatcg
     7141 gcacctgtcg cagcgcgcct aagagtcttt cgggcgtgga ggtttcatcc agatcaccca
     7201 ggtgatgttc gctcgcaaat ttcgtcgaac gcatgagtcg aaagattcgc cccagatttt
     7261 ttgtctgatc tttcattgga tccgctgcgt gcgcggagaa tcgggaatat tcagcatgcc
     7321 ccgaggccag ccagagtctg agtgtcgcac cggctaaatt caaactgccg cctgaattac
     7381 gttagacgac attcctcgaa ccagccacct tttggcgatc cctttcaggt tcgatggaga
     7441 aagctctgta atacatacga gttcatttcc ccgcacaaag ccaggatttc gctcgcgaaa
     7501 gaatcgaatg tacgcatttg tttccctgaa gagagtagcg gcaatgagtt ccggcctgag
     7561 ctctcctctg ctttcgggaa acacaaggag gccttgttcc tggtcatagt attgtggaaa
     7621 cataagcatg gattctgttt ttgcgatcgc ggccagatct tcgttctgcg agttcggatc
     7681 cggaaagaat ttcctggtga acaaaggtag atacagatac gttctgtggc cctttgaaat
     7741 tagaaaccag tagagaggct tcgtgcttgt tgccagaaaa cttccagcag cattgcagaa
     7801 acctctcaca agttcctgtg aaccccagtg tttgggcgtg atgattgtgt ctcccgagaa
     7861 aaggacgttg taatctggat gccttgtgcg cagtgcaatg gttgtgaaac cctggatgat
     7921 gtttgcgtgg tccatgagca atccaacaaa gtcctttgaa cccaggtctt cgtagaatat
     7981 ctcgcggctt acactggaat agttttcctc cagcagctgg aacatccttt ccgattcctg
     8041 atgcgaaatg gatgctcttt tacgcaggca gaagctgaga ctcacaagcc aatcctggcc
     8101 tgaatcaacg gatactccag cacaaaaacc agcgtccaga aagccatata ctgcggggcg
     8161 aaaataagca acgtcaggcg attagtccgc ttgtctgttt ccggtacgaa gtaaacacct
     8221 tgagccgggc ccgaggttga gatacgaaat cggtgaacga aatacatttc agtgatcagg
     8281 ctaagaatgg taagtaagat gatcagtgca tggaccgcgg gcagagaccg cgtggttagc
     8341 agagtaatgc agatcccaat ctgcaaagaa tcgtgcgcaa tcccatactc cgttttccat
     8401 cggatggtcc cgtagcaaag atacaattca acgacgcctc gcaagatcat gctcactgcc
     8461 aggagctgaa aacaaatgcg caactcgccg ggggcggtga aggatagaat ggatggtagt
     8521 agaatccaga gtgtgattgc gaaaatcagc cagaaggatt tgaccatgga gattggtcca
     8581 cccactgctt tacccgattt ctgcttcagg tagaacagag gagctgcagc ctggaatatt
     8641 agaagcatcg cggccatcat agcgcattcc tcttcatgcc ctgattctct ttttatggct
     8701 ggacctggct tttcgcccgc tcatcatttc cctgatttca cgagcgatcg catccgtttg
     8761 atgaaagtaa aaagcttctc ggccgaatct tttgatgttg tcagattgtt tcttgatcac
     8821 gcggacatct tgattgagaa tcatcgaaga aaggggcgca tagatcaacc tgacgatagg
     8881 cgctattgga tcaaatcgaa aggccatgta ggtgtagact ctcgtccggt actcgtctac
     8941 tggagtgcac tggctcatca cgataaagtg ccttttttca gagaaatagt aatccactct
     9001 agtagtagag ggcatgagaa aggcatccgt gtgctttgcc tggcttccag ccgggaccag
     9061 tagtttccca accaggccgt ctacctgtcg ttcgttcagg aattccgcag tcaccgattg
     9121 gttgtttcgc actatggata gagttgtatc agaaccttcg cgggtgcgaa agagtccaga
     9181 atggacgaac actgtgtgcg gaacgtccag gaagttttca atgcattgga acgcacttcc
     9241 atcgaattct ctttccataa accagttttg ccatcgcggg gctgaaaaca ttggaaactc
     9301 tggaaccggt cccggaactt tatctcccag gaagacccag atgaaacctt gactttctac
     9361 tgctgggaga ctgcgcgccg ccggtcgcgc gcccgattca gattcggccg ggatgcgaag
     9421 gcatttgcca tctgcaccaa aagtccagcc atgatagctg cattgcaggc cgtctttctt
     9481 gactttgccc agcgaaaggg caatgttgcg gtgaggacaa cgatcttcca gacacgccgg
     9541 cttttctccc gtgcgaaata gcaccagagg tttgcccaga atcgttcgcg aaacaggttt
     9601 tgacttaagt tcgcgcgaaa aacatgcaat gtaccaatag ttttctattg ggcgaaatgc
     9661 ttctatcatc tctgctccag ggtcgtataa cgtccagctg gcagatctat ttcttgaatt
     9721 tcctggccac accattgaat gactgcgcgg gcactgcctg taacggcaaa tagcaacctg
     9781 gtgtccgact gtgcggaaaa tccattgacc agtcgtactt ctctgtgttg cgaaatagat
     9841 ggcgatttca gttccaccgt gcttccgacc gcgtctcgat tgcaggtcct tccgtttccg
     9901 tgcaaaatca agccagccca ggacggttta ttgatatcgt ttctataaat gattggctct
     9961 ccaaattgat ctgtgaacac gagatctaga tctccatcgt tgtccaggtc cactgccgcc
    10021 actccgcgcg tatttgcgcg atgagtaacg cccacgtcgt ctgcgaaatc tgaaaaccgg
    10081 gatccctgat tgatatagat tcgatccgct tcatcaggat agatgcatgc gcctcgaagg
    10141 tccgcccagt tatcagcgta ggtatgaatt tccggaatgg agcgtgccag tttttcgttt
    10201 agataccagt agtccaggca ggtcgcataa cgtttgtccc atttattaga gaccattccg
    10261 tttgcctgca caatgtccag tttaccgtcc agatccagat ctgccatggc tgcgccccat
    10321 ccgaacctat gcggattgag tgcgccacgt tgcgacgcgc gatctttcat ggaaagattt
    10381 tgcgccccgg atgctgtgta gttcatccag aggagacttc cctctgcctg catgggatga
    10441 tgcacattgg aaacgtatac atcgtctgca ccgttgccgt ctatgtcccc catgcttgca
    10501 ttcattccct tatatgtatc caggcccagg tcttcaaaga attctccctg ttgtctgcga
    10561 aaacgtttgc cgccatcatt gatgtagcag tcgtctctac caaaatcatt tgcaatgtag
    10621 atgtccgtga aaccatctct gttcagatcc gcagtcccca cgctgagact ccaatgcgtt
    10681 tcatccaatc caacgtctgt tctggaaaaa ggatccggac cttttgtgcc tagatagagt
    10741 tcgttctttc caccgttgtt tgcgttgtgc catgaatgat gcatgaagtg gagcatgcgt
    10801 ctgtctcctt cataacgcgg ttctggaagt ttgaagatat tgagtggaat tttctctggc
    10861 aaatagtccg gcagatatac aggcaaagta tttgccacca gcacgtccag tctaccgtct
    10921 cgattgaagt caaacgcatt ggctgcaaga gcaatcgtat gatctttcgt tgcttgagtt
    10981 tctatttcgc gaaagcgaac aatgccttct tgcttgattc tgttttcaaa catatgactt
    11041 cttccgtagc cgaatccaac gaacaaatcc tgatctccgt cattgtcgta gtcgaagaaa
    11101 aatgcgaaag caggtaagcc gaactttgcc ggatcgttta gatatctctc cagatccggg
    11161 atgactattt tcttgaattg aaaattcccc tggttcaagt acaactttcc gcgatattcc
    11221 gtcgatttca gactctgcgt aaagaagaga tccggtttcc cgtccaggtc cacatctgcg
    11281 gatgcaaccg aatcaccgac tgataaaagc catttcgcaa tgtgctgaac gcgtggatct
    11341 acctgttcca gaatgttgct tttttcatac ccgattccgg actgagacga cgcgattctt
    11401 gtgaaatgaa ggcccggagg cgcggaacca cgcacattgg attgtcccga ttcatacatc
    11461 gcgaatgcgg gaatggcaaa aaagaaagtc agaatagcag caggaatatg gcttcgtagc
    11521 gccccactcc acgctcgagt tttccaggct gccacaacat agcggatggt cgcgagtgtg
    11581 aaggccgcgt aaaagaaagt aaataggcta aactttaagt ggtacgcaag gtcaaatatg
    11641 gccaggaaaa aaccaacgag aatttgagtt cgtttatcgg gtggacttgt ggctggatcc
    11701 gtaatcataa agaatgtaaa caaataaaaa gatggagaag taattgtgcc tatgaacagg
    11761 gcactggcag gtagatgata gcgaatgatg tagctacgaa tcagaagttg aatggcaaag
    11821 aatgtgagaa aggacaatgc cagccatttc ctgcttcctc tgtatccgaa aatcgtgact
    11881 cctgcggtaa gaatcgtaat cgacatgagc caggcagttt ctgcgcttcc ggtccactga
    11941 taggcgggtg caatgctgat ccatccgtct gcgaaaatca gactgaatac aattgcgaat
    12001 aaagaaggat tgtaaatgtg tcttccattc aaagtgaaga tatatttgga agcgatggca
    12061 atgagaacgg gcaaccatat gaaatggtat ccggaagaga agctgagtat aatggtcaaa
    12121 gagagagtcg tgatcactgc ggaaagagga aagatccact tacccttgag caacccgcag
    12181 aagatcacat ccagaagtgc ccctgcgctc agggcgacta tgattt
//
LOCUS       DAOQJL010000041        12080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2636_length_12080_cov_4.550852, whole genome shotgun sequence.
ACCESSION   DAOQJL010000041 DAOQJL010000000
VERSION     DAOQJL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_12080_cov_4.550852"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLG78_03100"
     CDS             <1..594
                     /locus_tag="PLG78_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22125.1"
                     /translation="EVRRECRYSLQANLYSIDVAVVQQSFYSSPSAPAVFQNSFSERL
                     QDGSLVIDIPIEVRRECRYSLQANLYSIDENEPTHWAVVQKVLDPGRQIVSFTFFGKI
                     FHDGGYSGKFQIRDIRGTCENMPYPAKWLGDPSKANQIANTPAVNEPPIIYIPFNDYA
                     YTTRSYNLDEFSNQEYTSAEKTKRLAELEEAAKASRE"
     gene            687..1187
                     /locus_tag="PLG78_03105"
     CDS             687..1187
                     /locus_tag="PLG78_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22126.1"
                     /translation="MKSEVRCADGSGIEMSVTYLENRRADPIAGSTDKSDYVTSVRRS
                     GREAKIQGWLLSEGAHCGKTAFFLGGEGDQFGVNPQLFSWDGARIKRVSESGPVEKFA
                     VSLDERYAALMFLREKIVFVEIQTGKTVAEEKISGTFDRLDWSGKKLLLSGPSGDLSI
                     LPRLAQ"
     gene            complement(1162..2073)
                     /locus_tag="PLG78_03110"
     CDS             complement(1162..2073)
                     /locus_tag="PLG78_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE22127.1"
                     /translation="MSSHQESSFRAILYAFLANFGIAAGKSVAAYLTGSGSMLAEAVH
                     SFADCGNQLLLFLGLHRAQMPPTRNHPLGFGKVVYFWSFLVAILLFTLGGIFSIREGI
                     HKLSTPEPLAHVPIALGVLGFSVILESFSMYGCIREIKKIKGTKTLLAWLKGSRNAEF
                     IVVLGEDTAALLGLAVAFTFVTLSYVTGNPIYDAVGSLVIGCVLVVIAFLISQRVKSL
                     MVGMSADPDLENRIEALIEKDKSIVKVFNIITQQFGPHLMLAAKIEMKPSLKISEAAV
                     KINELEKNLKKEFPEIRWCFIEPDVAK"
     gene            complement(2139..2534)
                     /locus_tag="PLG78_03115"
     CDS             complement(2139..2534)
                     /locus_tag="PLG78_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22128.1"
                     /translation="MKRNPMLSIIHAAICSGPVFFLIVSYFVGPKNAPQSFLAQIGWI
                     FGAGSAAASFFIPRLLAGGKEEINAGSEPSDSQGNLNFVQKIVQWAIVEAGALFNGVV
                     YFSTGDSGSAAVSVAMILLLIYFRPTASD"
     gene            2625..3911
                     /locus_tag="PLG78_03120"
     CDS             2625..3911
                     /locus_tag="PLG78_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNE22129.1"
                     /translation="MNTPYILGGAQTDFSRNWTKEGKTFMSMMREVVQDGLSQIGLDY
                     DEITRLNKENKIAVFVGNFDAEQYTQQGHLGAFLTEVHPAFYGVPGARYEAACASGSV
                     ALDAASARIRGGDYEVAVVLGIEIMKTVSSSVGGDFLGTAAYYEREAKGVQFPFPKLF
                     GKLADVIVERAKGVGEKRIMDALAEISRINYANAKLNPLAQTRTWFMSEEHAKDRGSQ
                     YNMAVGGRLCIADCSQVTDGAAITVVASPAYAKQFAQKKGIPMSKLAVIKGWGHRVAP
                     VTFDAKVAESKDNEYILPWTRQTVKDAYTRAGIDVDQIDVIETHDCFTSSEYAAISAF
                     GMTKPGEEYQAIEDGRIALDGPAKAGKKPINPSGGLIGAGHPVGASGVRMAVDLFRQV
                     TDQAGDYQVAGAKNGIMLNIGGSATTNMTFVISRAE"
     gene            4003..5223
                     /locus_tag="PLG78_03125"
     CDS             4003..5223
                     /locus_tag="PLG78_03125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE22130.1"
                     /translation="MKQGSFPVMAILDIIKTLQFPLPFEIHLPDGSHHKHGSGEPVFV
                     MRIKTDAALSDVMRQGSLGFGEAYMREEIDIEGDVQEVSRLGYALMETDALTPTFMEK
                     LKFLFVYFSRRNTREGSRKNIQAHYDLGNDFYSLWLDKEMQYTCAYFHDFKDSLEKAQ
                     LQKLEHVCRKVRLKKGDTVVEAGCGWGGFARYAAKHYGVKIRSYNISQEQIEYAREMQ
                     KKLKIPQSQVEYVLDDYRSIGSSGEKYDKFVSVGMLEHVGRENYYGLYEIIQKVVEKR
                     GMAIVHSIGRRFPRKPDPWLEKYIFPGCYIPSLSDMITPFESLPGHDLHVVDVENLRY
                     HYALTLDHWWMRLEKNAEKIRKQYGEAFVRMFRLYLRAAAAGFRYDELTLLQVVLSNG
                     SDNTAPLTRKHLYK"
     gene            5220..6389
                     /locus_tag="PLG78_03130"
     CDS             5220..6389
                     /locus_tag="PLG78_03130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE22131.1"
                     /translation="MSNQYDVIVVGAGPGGSNAARHFAKQGWKVLMLDRQKFPRVKIC
                     AGWITPPALRLLDLKPSDYPHTLQRFSSGSVVLGDRYYETDYGTTASYGIIRQEFDTF
                     LARRAQASGVEFLEEVRIKSIERGDSPDALVRVRSEDGTEWTTKLIIGAGGTGCPVAR
                     QWGEKSKEEIIVQATESETKLGADLLRTLTPYYGTTELFPEPDFFGYAWFVTKGDWVN
                     IGIGRFSHRTKQLDTHRDQFMEQLRKLGRLKGIEDKIVPFGRHAYKLYDEVPRKLFGD
                     RFMLIGDAAGFASKWAGEGIRPAIETGEFAARVGNAALKAQNLSANSLSPYKQLCDHA
                     YGPQKDTTMGRLISGLPGSVQTGVGTVICKSDYLRKKLIFEAAFGFEPVRDPVYL"
     gene            complement(6386..7510)
                     /locus_tag="PLG78_03135"
     CDS             complement(6386..7510)
                     /locus_tag="PLG78_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22132.1"
                     /translation="MLHKLAAIILVTASIALASPVHAQAFCQGTGCGAIPFSQDSLNS
                     LFFQFQYQYTNQLFSDIARAGTLANISSAPTGSVNLQKFTVGASGSAGFVPLQNKTLT
                     MPGVGVFQDVPSGGAYAIPHIFGGVNLGYLVGNEYDPYDSNGSTPSMLSLSRVDVYAT
                     GAEYREHYSRDNHNLKVVAGFYNRGFDVRYHLVEGANIAGGPLFRFLGVSLGTGYHLS
                     RLNIQAEQYQKYRVASLSGVQVFWDGYNYLNIQTKIKTYPVEIMSGMQLLYVLSLSVG
                     GGVTTNSGYADMNLTRIGPVYPSTDILNNLLSSAGVNLISNTFTSYLTMNISGRGTIP
                     KHVPYAKVSMDINLGPAKIFAEGISTRRAYGANAGVRVQF"
     gene            complement(7536..8273)
                     /locus_tag="PLG78_03140"
     CDS             complement(7536..8273)
                     /locus_tag="PLG78_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22133.1"
                     /translation="MKILKLLAMGTLLASLTNCFSIMHFLDLQENGTLNVQWSFRFSK
                     ALEDMGKKGDKAESGGLSQEVEKGKQELPGKLGSIAKDLKVEALQSDYDSGIRMSFAV
                     PQYLKLDFSKFQEAEFPYMPRYDAAKKQMIFHFTPAKKDKKEEKPAETKMPDAPKEGS
                     EETSEPATQGGDDMGKQIGKLFLSSVRYQIVLGKNLKVKSVSVSQDGVEKKLELQTIG
                     EQSMIDLPLFALYGDKELPFDLIVQLK"
     gene            complement(8328..10025)
                     /locus_tag="PLG78_03145"
     CDS             complement(8328..10025)
                     /locus_tag="PLG78_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE22134.1"
                     /translation="MIYTEVKALNTRRENLRWNAWGSLDNDFFYADRIGPILNYIRHT
                     LKMQETITPSLRLTDLRPAESKIKGTNLSSLQRIFGKNRVKTDNAERILHSAGRSYFD
                     VMRLRGNLLKTYVDAVVYPETELEIEKLLKLAVQRNWAIIPFGGGSSVVGGVEAKSGG
                     KKAIVCVDMTRMNRLIALNPVSSVATFEAGIYGPDLELALAKQGYTLGHFPQSFEYST
                     LGGWVAARSAGQQSGRYGKIENMVAAIRMVTPGGLFTTGHYPAASTGPDLNQILCGSE
                     GTLGIITAVTVKLHELPSERTYGGFLFPDFATGLEFIREANQLELSFSMLRLSDEGET
                     SQLESFGRIVSHVQNPGAGERIKRFAKDTIEGIVLQSYGLSERKCLIVAGADGPDSKE
                     AIARLKKLAPRFRGMYAGASPGKKWLKGRFNMPFLRNYLLEYGIGVDTMETAVPYGRA
                     MKVHDEVLAAIQESIPGAIGICHASHSYIDGVCLYFTVMFPMDLDHPVEQWTQLKTAV
                     SDAMLANGGNTSHHHGIGNDHREHFRRQVGPAALATLQSLKKTLDPRGNLNPGKLMN"
     gene            complement(10022..10828)
                     /locus_tag="PLG78_03150"
     CDS             complement(10022..10828)
                     /locus_tag="PLG78_03150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE22135.1"
                     /translation="MSSVLEAIERLNRLEKLVLPDLSQALVRIQSGNLGIKTKSNDKD
                     LVTVADVESENRLTSFIREHWPEDSILAEETGGSIGRDLTWIIDPVDGTVNYAHGLPL
                     YSVSVGIALDGKPLAGIVHMPSLGTTYKGAVGHGATKNGNPIRVSETNAASQALVVTG
                     FPYDRATILEALIAGVESVLKNCRGIRRTGSASIDLCWLAEGSFDAHYECNLSPWDTC
                     ASEVILQEAGGRITTFAGEEHRLTDKTLLATNHLLHEAMLQIIAPLRSGL"
     gene            11126..11836
                     /locus_tag="PLG78_03155"
     CDS             11126..11836
                     /locus_tag="PLG78_03155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE22136.1"
                     /translation="MDELNIQIILIRHGETEYNREGRIQGTLDSPLTERGRLESSTLG
                     VSMRDWLPPVHQWLVSPQGRARESSRLIRDHFANGGQAVRVLPAEELEESAREINCGD
                     YEGKITETLDQSIIQKVRELADFPYPQGESLVDVMNRGKFVLARVIESSQRAHNAHPD
                     LNEYRVVLVSHGNFIRAFGALLTRLGPEFALRAFQSNTGVNRLVSRDGGRGFKILTWN
                     DTSHMNQHPTEAANLGTA"
     gene            complement(11837..>12080)
                     /locus_tag="PLG78_03160"
     CDS             complement(11837..>12080)
                     /locus_tag="PLG78_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNE22137.1"
                     /translation="EFTGEQLDKTITSSDWGNPKLSPEQLKYAEEDIKYLFKIKRKVH
                     EMLEREGRMGFYKSCVEFLPVRIELDRLGYGDIFEH"
BASE COUNT         3010 a         2926 c         3139 g         3005 t
ORIGIN      
        1 gaagtgcgtc gcgaatgcag atacagtctg caagcgaatc tctactccat agacgttgca
       61 gtcgttcaac agagtttcta ctccagtcct tctgctcccg ctgtgtttca gaatagcttt
      121 tctgagaggc tacaggatgg atcgctcgtc attgatattc caatcgaagt gcgtcgcgaa
      181 tgcagataca gtctgcaagc gaatctctac tccatagacg aaaatgaacc aacgcactgg
      241 gccgtagttc aaaaggttct agatccaggc agacagattg tttcttttac gtttttcgga
      301 aagatctttc acgacggcgg ttatagcggt aagtttcaaa tacgcgacat ccgcggcaca
      361 tgcgaaaata tgccataccc ggcaaagtgg ctcggggatc cgtccaaagc aaatcagatt
      421 gcaaacacgc cggccgtcaa tgagcctccc atcatttaca tccctttcaa cgactacgcc
      481 tacaccactc gctcttataa cctggacgaa ttctcaaatc aggaatacac gtccgcagag
      541 aagacaaaaa ggttagcaga actagaagaa gccgcgaaag cgagtcgcga atgatttatt
      601 tttttcgcac acctttcatg tttttcccga ggctcgtgac aatctcagtc ttgctgctga
      661 tggtcggttg ccattcgcgt actgaaatga agagcgaagt gcgttgcgca gacggatctg
      721 gaatcgaaat gagtgtcacg tatctagaaa acaggcgggc ggatccaatc gcaggcagta
      781 cggataagtc agactatgta acatcggttc gtcgatctgg aagagaggcg aagattcagg
      841 gatggttgct ttcagagggc gcgcattgcg gaaagactgc cttctttctg ggtggagaag
      901 gagatcaatt tggagtcaat ccgcagttgt tttcctggga cggggcccga atcaagcgag
      961 tttcagaaag cgggcccgtg gaaaaattcg cggtgtcttt ggatgaacgt tatgcggcat
     1021 tgatgtttct cagggagaag attgtttttg ttgagattca aacggggaag actgtggccg
     1081 aagaaaaaat cagcggaacc tttgacaggc tggactggag cggaaagaaa ctccttcttt
     1141 ccggcccgtc cggagacctg tctattttgc cacgtctggc tcaatgaaac accagcggat
     1201 ttccgggaat tccttcttca gattcttttc gagttcgttg atcttcaccg cagcctcgct
     1261 gatttttagg gatggtttca tttctatttt tgcagcgagc atcaggtgag gaccgaattg
     1321 ctgcgtgatg atattgaata ctttgacaat ggacttgtct ttttcgatca aagcttctat
     1381 tctgttttct agatctggat cagcggacat tcccaccatg agcgatttca ctctctgaga
     1441 aatgagaaaa gcaattacaa ccagaacaca tccaatcacg agtgatccca cggcatcgta
     1501 gattggattt ccggttacgt agctaagcgt gacaaaggtg aaggcaactg caagacctag
     1561 aagagcggcg gtgtcttccc cgagcacgac aatgaactcg gcgtttctag aacctttcaa
     1621 ccacgcaagc agggtcttgg tgcctttgat tttcttaatc tcgcgaatgc agccgtacat
     1681 agagaagctt tcgagtatta cagaaaaacc gagcacacct agcgcgatcg gcacgtgagc
     1741 gagaggctcc ggtgtgctga gtttgtggat tccctcacgg atggagaaaa tgcccccgag
     1801 tgtaaatagt agaatggcca ccaggaagct ccaaaagtac acaaccttac caaacccaag
     1861 cggatgattg cgagttggcg gcatttgcgc gcgatgaagg ccaagaaaaa gaagcagctg
     1921 attgccgcag tctgcgaagg aatggactgc ttctgcaagc atactgcctg agccagtcag
     1981 gtatgcggcc acactttttc ccgcagcaat tccgaagtta gccagaaatg catataggat
     2041 cgcgcggaag gatgattcct ggtgactgct catgggttcg ttctcctgaa atgctagtaa
     2101 ttctcgaata cttacaaaat attatgaatc tgcgtgtctc aatcggatgc ggtcgggcga
     2161 aaataaatca acagtagaat catcgcaacg gaaactgcag cagagcctga atcgcccgtt
     2221 gaaaaataga caacaccgtt gaacagtgct cccgcttcca caatggccca ttgcacaatc
     2281 ttctgaacaa aattcaggtt tccttgcgaa tcggaaggtt cacttcccgc attgatttct
     2341 tccttgcctc ccgcaagtaa gcgcgggatg aaaaaagaag cggccgcgct gcccgcgcca
     2401 aagatccacc cgatttgcgc gaggaacgat tgtggcgcgt tttttggtcc tacgaaatag
     2461 gatacgatca gaaaaaaaac aggccccgaa caaatggccg catggatgat tgagagcata
     2521 ggattgcgtt tcacgctgtc ccattgacct gattgcacca cggggtcaac gccattcctg
     2581 gaaataaata ttgacgctcg cattttgtac acgttccctg caatatgaac acgccataca
     2641 ttctgggcgg cgcccagacg gatttttcaa gaaactggac taaagaaggg aaaaccttca
     2701 tgtccatgat gcgcgaagtc gttcaagacg ggctgtctca aattggcctg gattatgacg
     2761 aaatcacacg tcttaataaa gagaacaaaa ttgctgtatt cgttggaaac ttcgatgcag
     2821 aacaatacac ccagcagggc caccttggcg cattcctgac ggaagtgcac ccggccttct
     2881 acggagttcc cggcgcccgc tatgaagctg cgtgtgcttc cggatccgtc gctctcgatg
     2941 cagcatctgc caggattcgc ggcggtgatt acgaagtagc ggtcgtactt ggaatcgaaa
     3001 tcatgaagac agtgagctct tcggttggtg gagatttcct tggaactgca gcatactacg
     3061 aacgcgaagc caagggagtt caattccctt tcccaaaatt gtttggaaaa cttgcggatg
     3121 tgattgtcga gcgcgccaaa ggcgttggcg aaaaacgtat catggacgct cttgctgaaa
     3181 tctcaagaat taactacgcg aatgcgaaac tcaatcctct tgctcaaaca agaacatggt
     3241 ttatgagcga agagcatgcg aaagatcgag gcagtcagta caacatggct gtgggcggaa
     3301 gactctgcat tgcagattgt tcccaggtaa ctgatggcgc ggctatcact gtggtggcca
     3361 gtcctgctta cgctaaacag tttgcgcaga agaaaggaat tccaatgagc aaactggcag
     3421 tgatcaaagg ctggggccat cgcgttgctc ccgttacgtt tgacgccaag gttgctgagt
     3481 ctaaagacaa cgaatacatt cttccatgga cgcgccagac tgtgaaggat gcatacacgc
     3541 gcgcagggat cgatgtggat caaatcgatg tgattgagac gcatgactgt ttcacttcca
     3601 gtgagtatgc tgcgatttct gctttcggaa tgaccaagcc cggcgaagag tatcaggcaa
     3661 tcgaagatgg caggatcgct ctggacgggc cggccaaagc gggcaagaag ccaatcaatc
     3721 cttccggcgg actgatcggt gcaggccacc ccgtgggagc atccggtgtg cgtatggctg
     3781 tggacctttt ccgtcaggtg acagatcagg caggagacta ccaggtggca ggtgcaaaga
     3841 atggcatcat gctgaacatt ggtggaagtg ccacgaccaa catgactttt gtaatcagcc
     3901 gagccgaata agcgctctac ccgattacac tgtgtcagaa aaaaggccgc gcttgcggcc
     3961 ttttttgttt catggggtca aaacatctgg acggtgcggg ctttgaaaca aggatcgttc
     4021 cccgtcatgg cgattctaga tataatcaag acccttcaat ttccacttcc atttgaaatt
     4081 catctgccgg atggcagcca tcataaacac ggttccggag aaccagtgtt cgttatgcga
     4141 atcaagacag atgcagcact cagcgacgtg atgcgtcagg gcagtcttgg tttcggtgaa
     4201 gcgtatatgc gtgaggagat cgacatcgaa ggagatgtgc aggaagtttc gcgccttggc
     4261 tacgcgctta tggaaacaga cgcgctcact ccaactttca tggaaaagct gaagttcctt
     4321 ttcgtgtatt tttcccgcag aaacacgcga gagggttcgc gcaaaaatat tcaagcacac
     4381 tatgatcttg gaaacgattt ctattcactg tggctcgata aagaaatgca gtacacgtgc
     4441 gcatacttcc acgatttcaa agactctctt gaaaaagctc aactccaaaa gctggaacat
     4501 gtgtgccgaa aggttcgcct gaaaaaaggg gacacagttg tagaagctgg ttgtggatgg
     4561 ggtggttttg cacgttatgc ggcaaagcac tatggggtta agattcgatc ctacaacatt
     4621 tcgcaagaac agatcgagta tgctcgggaa atgcagaaga aattaaagat tcctcaaagc
     4681 caggtagagt atgtcctgga cgattatcgc agcattggat cttcaggtga aaaatacgat
     4741 aaattcgtat cagtcgggat gctggagcac gttggccgcg aaaactacta cggactctat
     4801 gaaatcattc aaaaggtagt agagaagcgt ggcatggcga tagttcattc catcggaaga
     4861 agattccctc gtaaaccaga tccatggctc gagaaataca tctttcccgg atgctacatt
     4921 ccttctcttt ccgacatgat cacgccgttt gaatcgcttc ccggtcatga tctgcatgtg
     4981 gtggatgtgg agaatctcag atatcattac gcacttactc tggatcactg gtggatgagg
     5041 cttgagaaga acgcagagaa gattcgcaaa caatatggag aagcattcgt gcgaatgttt
     5101 cgcctctatc ttcgcgctgc agctgcgggc tttcgctacg atgaacttac acttttgcaa
     5161 gtagttctca gcaatggcag tgacaacaca gcacctctca caaggaaaca tttgtacaaa
     5221 tgagcaatca atatgacgtc attgttgtag gcgcgggtcc gggcggatcc aacgcggccc
     5281 ggcattttgc gaaacagggt tggaaagttc tgatgctgga tcggcagaaa tttccgaggg
     5341 tgaagatttg tgccgggtgg atcactccgc ctgcgttgcg cttgctcgac ttgaaacctt
     5401 ccgattatcc gcacacgctc cagcgcttct cttctggttc tgtggtcctg ggggatcgtt
     5461 actacgaaac cgattacgga acaactgcaa gctacgggat catcagacaa gagtttgata
     5521 catttctggc cagacgtgcg caagcctccg gggtcgaatt tctggaagag gttcgaatca
     5581 agtcgatcga acgcggggat agccccgacg cgctcgtcag ggttcgcagc gaagatggaa
     5641 cagagtggac cacaaaactg atcattggtg ccgggggcac gggatgtccg gtggcgcggc
     5701 aatggggaga aaaatcaaaa gaagaaatca tcgtacaggc aacagaatca gaaaccaagt
     5761 taggcgcgga tctacttcgg acactcactc catattatgg aacaacggaa ttgttccccg
     5821 aacctgactt cttcggatac gcctggtttg tgaccaaagg agactgggta aacattggaa
     5881 tcggccggtt cagccacaga accaaacagc tggacacaca cagagaccag ttcatggaac
     5941 agctgcgcaa actcggacgt cttaagggca ttgaggataa aatcgtgcca ttcggtcgtc
     6001 acgcatacaa gttgtacgat gaagttccgc gaaaactgtt cggagacaga ttcatgctga
     6061 tcggagatgc tgccggcttt gcttccaaat gggcggggga aggaattcgt ccggctattg
     6121 aaactggcga atttgcggcc cgagtcggta acgcggctct caaggcacaa aacctgagtg
     6181 ccaattcgct ttcaccttac aagcagttat gtgatcatgc gtatggtccg cagaaagaca
     6241 caacgatggg gcgcttgatt tccggccttc ccgggagtgt tcagaccggt gtgggaaccg
     6301 tgatttgcaa atccgactat cttagaaaga agctgatctt tgaagctgct tttggctttg
     6361 aacctgtgcg cgatcctgtc tatctttaga actggacgcg cactccggca tttgctccgt
     6421 atgctctcct ggtggagata ccttccgcga agatcttcgc gggtccgagg ttgatgtcca
     6481 tactcacttt tgcataagga acgtgttttg gaatcgtacc tctgccagaa atattcatgg
     6541 taaggtaaga tgtaaacgtg ttggagatca gattcactcc tgcagatgac agcagattgt
     6601 tcaatatgtc cgtggacggg tagaccggcc caatgcgggt aagattcatg tccgcataac
     6661 cgctgtttgt tgtaacgccc ccgccaacag agagactcaa aacatagagc aactgcatcc
     6721 cgctcataat ttcaaccggg tatgttttga ttttcgtctg gatattgaga tagttgtagc
     6781 cgtcccagaa gacctgcacg ccgctcaagg acgcaacgcg gtatttctgg tactgctccg
     6841 cctgaatgtt cagccgagag agatggtagc cagtgcccag ggaaacgccc agaaatcgaa
     6901 atagtggacc accggcgatg tttgcgcctt ccacgagatg atagcgaaca tcaaaacccc
     6961 gattgtagaa tcccgccacg accttcagat tatgattgtc ccgtgagtaa tgctctctat
     7021 attccgctcc ggtcgcatat acatcgacgc gagatagaga tagcatggat ggagtcgaac
     7081 cgtttgaatc atacggatcg tattcatttc cgacgaggta gccaagattg actcctccga
     7141 aaatatgtgg gatcgcatac gctcccccgg aaggcacgtc ctggaatact cccactccgg
     7201 gcatggttag agttttgttt tgaagcggaa cgaatcccgc acttccactg gcgccaacag
     7261 taaatttttg taagtttacc gaacctgtgg gggcgctgct gatgtttgcc agagtgcctg
     7321 cgcgtgctat atctgaaaaa agctgattcg tgtactgata ctgaaactga aaaaacaggc
     7381 tgttgagaga atcctgcgaa aacggaatcg cgccgcaacc agttccctga cagaacgcct
     7441 gagcgtgcac cggactggca agcgcgatgg aagctgtgac gagaatgatt gccgcgagtt
     7501 tgtgtaacat cgtgatcctc tgagcaccac cgcgcttatt tcaattgtac aatcagatca
     7561 aatggcagct ctttgtctcc atagagagcg aagagcggaa gatcgatcat ggattgctca
     7621 ccgattgttt gcagttcgag tttcttctct actccgtcct ggctaacaga cactgatttt
     7681 actttgagat tttttcccag cacgatctgg tagcgaacag aggagaggaa gagtttgccg
     7741 atttgtttgc ccatatcgtc tccaccttga gtggctggtt ctgacgtttc ttccgatcct
     7801 tctttggggg cgtcgggcat ctttgtttct gccggctttt cttccttttt gtcttttttt
     7861 gcaggcgtaa aatggaagat catctgcttc ttcgccgcat catatcttgg catgtaagga
     7921 aattcggcct cttgaaattt ggagaaatcc agttttagat actgcggaac cgcgaaggac
     7981 attcggatgc cggagtcata atctgattgc agcgcttcta ctttcagatc ctttgcaatg
     8041 gacccgagct tgcccggaag ctcctgtttt cctttctcca cctcttgcga aagtccgccg
     8101 ctttcagcct tgtctccttt ctttcccata tcttccagcg ctttggagaa gcgaaagctc
     8161 cattgaacgt tcaaagttcc attttcctgc agatccagaa aatgcatgat agaaaagcag
     8221 ttggttagcg aggcgaggag agtgcccatc gccagtagtt tcaagatttt caaaattgtt
     8281 ctcctcagat tctttatagg acgtgcaaaa agacccgtgc gttcgcgcta attcatcaat
     8341 ttcccaggat tgaggtttcc acgcgggtcc agagttttct tgagagattg aagcgtcgca
     8401 agagcggcag gcccgacttg cctgcgaaaa tgttcgcgat gatcatttcc aattccatga
     8461 tgatggctag tattcccacc gtttgcaagc atcgcgtcgg acacggcagt cttgagctgt
     8521 gtccattgct cgactggatg atcgagatcc atgggaaaca tcaccgtaaa atagagacaa
     8581 accccgtcga tgtaagagtg acttgcatgg caaatcccga tcgcgccggg aatgctctcc
     8641 tggatcgctg cgagcacttc gtcgtgcact ttcatagcgc gcccgtaagg caccgcagtc
     8701 tccattgtgt ccacgccaat cccatactct agtagatagt tgcgcagaaa cggcatgttg
     8761 aagcgtcctt tgagccattt ttttccaggg cttgcgccgg cgtacattcc gcggaatcgc
     8821 ggagcgagtt tctttagtct ggcgattgct tctttgctgt ctggaccatc cgcgcccgcg
     8881 acgatcagac acttgcgttc ggaaagccca tacgattgaa gaacgatgcc ctcaattgtg
     8941 tctttggcga atcgcttgat ccgttctccg gcgcctggat tctgaacgtg cgatacgatt
     9001 ctgccaaatg attcgagttg agaagtttct ccttcatcgc tgagacgaag catcgaaaaa
     9061 gaaagttcca gctggtttgc ttcgcggatg aactcgaggc cggttgcgaa atcgggaaag
     9121 aggaagccac cgtaagtgcg ctcagatgga agctcatgca gttttactgt taccgcagtg
     9181 atgattccca gagtcccttc gcttccacat aatatttgat tcagatcggg cccggtggaa
     9241 gccgcagggt aatgcccggt tgtaaagagt ccgcccggag tgaccatgcg aatggctgcg
     9301 accatgtttt cgattttgcc gtacctgccc gactgctgac cggcgctgcg ggccgcgacc
     9361 cacccaccta acgtcgagta ttcgaaggat tggggaaagt gaccaagagt atagccttgc
     9421 ttggcaaggg cgagttccag gtcaggtccg tagattcctg cctcgaaagt tgcgacagaa
     9481 ctcacagggt tgagtgcgat gagacgattc atcctggtca tgtccacgca gacgattgcc
     9541 tttttgccac cggactttgc ttccactcca cctacaaccg agctacctcc tccgaatggg
     9601 atgatggccc agtttctctg caccgcgagt ttgagaagct tctctatttc aagctccgtc
     9661 tctggatata caactgcgtc cacataagtt ttcaggagat tgccacgaag gcgcataacg
     9721 tcgaaataac ttcttcccgc tgaatgcaga attcgttcag cgttatctgt cttgacacgg
     9781 ttttttccaa agattctctg aagagaggaa agatttgttc ctttgatttt tgattccgca
     9841 gggcgaagat ccgtgaggcg aagagaagga gtgatagttt cctgcatctt gagcgtatgg
     9901 cgaatgtagt tcaaaatcgg gccgattcta tccgcgtaga agaagtcgtt gtccagcgaa
     9961 ccccacgcat tccatcgcag gttttcgcgc ctggtgttga gcgcctttac ttcggtgtag
    10021 atcatagccc gctcctcaat ggtgcgatta tttgaagcat agcttcgtga agtaggtgat
    10081 ttgttgcaag cagggttttg tccgttagcc gatgttcctc ccccgcaaat gtagtgattc
    10141 tcccaccggc ttcctgtaag ataacttctg aagcgcacgt atcccagggg ctcaagttgc
    10201 attcgtagtg ggcgtcaaaa cttccttctg ccagccagca taaatcaatg gatgcagacc
    10261 ctgtgcgcct gattcccctg cagttcttga gaacagactc gactccggcg attaaagctt
    10321 ccaggattgt agctcgatcg tagggaaatc cagttactac aagagcttgc gatgcggcgt
    10381 ttgtttccga cactcggatg ggattgccat tcttcgttgc gccgtgtccc acggcaccct
    10441 tgtacgttgt tcccagagaa ggcatatgaa caattccggc cagcggctta ccgtcgaggg
    10501 cgatcccaac agaaacgctg tatagaggga gaccgtgtgc gtagtttact gtgccgtcca
    10561 ctggatcgat gatccaggtc aggtcacgac caatgcttcc gcctgtttcc tctgccagaa
    10621 tggaatcttc gggccagtgt tcacgtatga atgacgttag gcgattttcg gattccacgt
    10681 ctgccacggt gacgaggtct ttgtcatttg atttggtttt gattcccagg tttccgctct
    10741 ggattcgaac cagggcctgg cttagatcgg gcagtacgag cttttctaaa cggttgagtc
    10801 tttcaatcgc ttcgagtacg gaactcaatt tgcctcaggg aaacaggcgc agccgcccgg
    10861 ctgacaggat ctttcgggag ccgaactctt gccgcttgcg cccagaactg ccaggggttt
    10921 gaatacttga agagtccgag tggaattgca tttttcgcac accgcagatt tacctgtttc
    10981 tcggtctgcg aggctcatct tgctgtcgaa tacctggccg caatcctgac actcgaatgt
    11041 gaacattgcc atatgcccag tagaacattc atttaggcga ggggcaagga aaaaaccgat
    11101 tgcggcagga gcgaagccga ctgcgatgga tgagttgaat attcaaatca ttctcattcg
    11161 acacggggaa acagaataca atcgagaagg aagaatccag ggcaccttgg attcacctct
    11221 aacggaaaga ggacgcctcg aatccagcac actgggtgtt tctatgcgtg actggttgcc
    11281 acctgttcac caatggctgg tgtcgcctca gggtcgggcc cgggaaagtt cgcgtcttat
    11341 ccgagatcat tttgcaaatg gtgggcaggc tgtgcgtgtc ttgccggcgg aagaactgga
    11401 ggagtcagcg cgtgagatca actgcggcga ttacgaaggg aagatcacgg aaactctcga
    11461 tcagagtatc atccagaaag taagagagct cgcggatttt ccttatcccc agggagaaag
    11521 cctcgtggat gttatgaatc gcggcaagtt cgttcttgct cgagtgattg aatcgtcaca
    11581 gcgcgcgcat aatgcgcatc cagatctaaa cgaatatcga gtggttctgg tttcgcatgg
    11641 aaattttatt cgcgcgtttg gagctctctt gacccggctg gggcctgagt ttgcgcttcg
    11701 tgcattccag agcaacacgg gagtgaatcg tctggtttcg cgtgatggag gtcggggctt
    11761 caagattttg acatggaacg ataccagtca catgaatcag catccaacgg aagccgcgaa
    11821 tctgggcact gcgtaatcag tgttcgaaaa tgtcgccgta tcccagtcga tcgagttcaa
    11881 tgcgaacagg aagaaattca acacagctct tataaaagcc cattctgcct tcgcgttcaa
    11941 gcatctcgtg aaccttgcgc ttgattttga agaggtactt tatatcttct tccgcatact
    12001 tcaattgctc tggtgagagt tttgggttgc cccagtccga agatgtgatg gtcttatcca
    12061 gctgttctcc ggtgaattcg
//
LOCUS       DAOQJL010000042        12053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2647_length_12053_cov_4.356559, whole genome shotgun sequence.
ACCESSION   DAOQJL010000042 DAOQJL010000000
VERSION     DAOQJL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2647_length_12053_cov_4.356559"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..341)
                     /gene="rpsT"
                     /locus_tag="PLG78_03165"
     CDS             complement(69..341)
                     /gene="rpsT"
                     /locus_tag="PLG78_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNE22138.1"
                     /translation="MANLRASKKDIRKTRKRNEQNSQSRARLRTLDKKIRTLVAEGKT
                     EDAATAFKSYTQFLARAGQKNLIHHRQADRRKSRMAALLNRQKKSA"
     gene            481..1686
                     /locus_tag="PLG78_03170"
     CDS             481..1686
                     /locus_tag="PLG78_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE22139.1"
                     /translation="MLWQLTNGTLLLPDGPRKLSGLLVSGGRIEEVLAPGDDQPDILT
                     LNLHGALVIPGLINSHDTLMATYLPTKGENWPHLNWLSFDIETKRSALFRERMLLDVE
                     TLYRMGSLKNLESGVTAVVDHVPDFVRNPFLSSMSVEILPDFGIAHSVCSYSLGWGQG
                     LEAEHKRAVENDLPFILHIAEGFDPESELSLRVLDRAGCLSEHTVLVHGLSLSNDDLD
                     RIAEAGAHFVWCPSANLFAYNRTVKISEFLTRGIPVCLGTDAAVTGSTHMLSEIQTAL
                     KVYKDQTGEDLPDSDLFAMLTTNPARAFRWAGRGSLKPDSLADFVVLSCPSLREAKPE
                     DIYLVVRGGQPVYGSEDLEPLFISAAVHVERVDIGGTRKIVQKGVKDLRETVRQATGR
                     ESFPFLSGW"
     gene            1688..2767
                     /locus_tag="PLG78_03175"
     CDS             1688..2767
                     /locus_tag="PLG78_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE22140.1"
                     /translation="MPIARNYNGGSIVYFAGDKGDEVYVLQAGRVILISTALDTGEEI
                     REDVQIGEFFGVKSSLGKFPREETAQVIGKTTCIVFKLHEFEQLVMKNTHLILKMLRV
                     FSKQLRNIHRQIRDILKAGAAKDPSFELMSVAESFYRSGNVDHAVYALEKYLEVYADG
                     DYTDRAHDLLSQARKGLTYPHGYPPLQIADQKHKVDASFVTRSMASADSLGDDPFALE
                     EGAPAARPAEKEPGAADILMDGLTLMSQNKADAALAKFNECLAIKKLHTADDQEAAER
                     AQYEKGRAELKLGKVDDASNSFSIYLKKFPTGEFMKHAIFQLGVAAEAKGNKERARTL
                     YHKVATMPPPDDATRDARKRLEKLG"
     gene            2764..3408
                     /locus_tag="PLG78_03180"
     CDS             2764..3408
                     /locus_tag="PLG78_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE22141.1"
                     /translation="MMLLPESLFDKFGKVFEPGQIIFCEYEPGNDFYFIQEGRVKITK
                     TIGNAQKTLDVLSQGDIFGEMAILEEEPRSATAIAVDKVRTLNFNRANFDTLMKSNPQ
                     LAFRLLVIFSNRINDARRRLQILLLDDTTSKVVDVFLMLAEKDAHYGQVKSMVFNTTV
                     DDIASWCGQPIDEVQKIINTFVRQGKVELFADRIVVSNINDFNRLIQAKRKSLL"
     gene            complement(3377..4624)
                     /locus_tag="PLG78_03185"
     CDS             complement(3377..4624)
                     /locus_tag="PLG78_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE22142.1"
                     /translation="MQIMLSGGGALDFYHMMASASSNYRLAPFFQPIVSIGDGSIYGY
                     EVLGRFVDESGHFTSMGPYFADPTVPGHEKLIRSRKVRELAFLHARDVNYQGRLFINV
                     KPSWLMQVFRAGSEIPTLRLLEELGLNPRNVVIEITEDEFEGDLVILDSVISRYREAG
                     CKIAIDDFTFHNFDRLIHLKPDIVKIDIRLVKKSVEKKEYQKLISSISDFAQEIGISV
                     LFEGVEFEEELVNSIDAGGSYIQGYFFSQPEPDLQIADTYRDRIRAVLDSVIQNQRQE
                     NYSLLSLEFHLNELMEEMLKKISFEGDLDEALNRLLGSLPSACYRVYVCDQFGNQRSS
                     NFLRSSNAVFTRDDDFRSRNWGWRPYFFHNLARMDRFRRGFMSNRYIDQETKKETYTY
                     SHPLYGNLFIFLDFTRGSFSSPE"
     gene            4816..5154
                     /locus_tag="PLG78_03190"
     CDS             4816..5154
                     /locus_tag="PLG78_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22143.1"
                     /translation="MEFTRKDRGPCSVFHITGDVDLYNSDRLKDALLQHLDSAGKSII
                     LDLTHTDYLDSTGIGAIVAAWNKAKVINQQVMLSSVSESIRRVLSVMQIDAHIQIFEH
                     TDAALASLRR"
     gene            5210..6511
                     /locus_tag="PLG78_03195"
     CDS             5210..6511
                     /locus_tag="PLG78_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22144.1"
                     /translation="MSKKASWKEIFGWAMFDFANSSYTTVIITVIWGDLFIRLVVGDG
                     PDFRRGNLAFGIALFISYIFCVITGPIFGAIMDFSAAKKKFLFMSYMLTVVSTCSLFL
                     VSPGMEVLGVVLLIISNFGFAAGESFVASFLPSLGEKEELGKISGIAWGIGYLGGLVS
                     VILVGLLGSVEIENFERLRYVGPMTGIFFMVAAIPTFLWLREPGVAHGLPQGQSYLGV
                     GFARLRQTLSEVKDFRDLGIFFVSLFFASAGLSIVISFAFTYGAQVIHWTDATKTMMF
                     VITQFTALFGALGFGYLQDKIGAKITYMMTLVLWIIAIICIFLTDSIAASLGVKPEQA
                     FLIVGCLAGAGLGATQSAGRALVGVFSPESKAGEFFGLWGLIGKLAAAIGVFGVAALQ
                     TQLGLRLAILFCALLFVAGLLFVIPVNEKRGQAAAEKHEGE"
     gene            6498..8219
                     /locus_tag="PLG78_03200"
     CDS             6498..8219
                     /locus_tag="PLG78_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE22145.1"
                     /translation="MRASNRTIAIAAVFITAAVTAFKFFFAIQLPLHPDEAYYWDWSR
                     HLDWSYYDQGPGVAFYIRIFTSVLGNTIPALKLAALSAGLIAHILLFFAARRAGVSAA
                     SSLLFVCCTILVPGFFSGSFLIMHDSVLILFWCAAFLFMLRWIETDSSADLFALFACM
                     ALGFLGKHTMVFFGLSFVIWFVLTPASWRELRNMRLYAAIGFAAILSSPFIIWNMQNN
                     WDGLGAILNLRSSGGAFANKSSTGAFVVGQALLLSPVWFALFLGFAFWILFLQLRNRE
                     IPRSLRSAMEMFRTDRSPEAAKNRFIWIIALILPVFFLALSRRREVQPNWTFASYAGI
                     MLIFFVYRLEQKKWTRVILAAGAVVALAMNTFFLFSAPLTKALGIPQNPRTARILPHL
                     RLAGSKEVIEAVDRQRQKIDPSAGILANRYQDAALAGFYLAGQPQVVSMNVMQRNQYS
                     YWPGPEPGKNYLVFMVQDNTCEKATFFFQPVLYYMFEKIEEYPESEIVLDGVAVKRYQ
                     TWYVQNYKRDWKGLLLYYMVDAAILDFMPNMRGYNPGLNSAEGMASIQNYMNQNYFMR
                     KGDVSCD"
     gene            8210..8626
                     /locus_tag="PLG78_03205"
     CDS             8210..8626
                     /locus_tag="PLG78_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22146.1"
                     /translation="MRLIALILALLTFCAKKEKLPDPGDYRAFIQGSAHLLCGKMVEC
                     YSGLYFSISPELRRQITVEACEKAALRDLDRKLARHTQSMKNLSVICYQAILDSPCKE
                     VGNAFFWNPSCFALRNESEKAFADVPMPNLFVLEKK"
     gene            complement(8633..9718)
                     /locus_tag="PLG78_03210"
     CDS             complement(8633..9718)
                     /locus_tag="PLG78_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNE22147.1"
                     /translation="MAALKKKAARKPVKVATKSRGKPASKKPAKKAGVAKGQSAKAAP
                     VVEVVKKATRDGYGEALAELGKTRPEVVVLDADLSESTRTHKFARAYPDRFFNVGVAE
                     QNLVGMAAGLSLSGLVPFASSFAMFLSGRAWEIVRNSVAYPGLNVKLVASHGGITVGE
                     DGASHQCIEDFAVMRAIPKMRVFVPSDFTEAKQMIAAVAAMKGPCYIRVSRAATPVLS
                     RPPSYKFQEGKGELRRDGKDVTIIACGVMVAEADLAAEKLAKQGIQAAVINMASIKPI
                     DEKLVLAYAKKTGAIVTSEEHNVIGGLGSAIAEVISENQPCVVLRNGMQDEFGQSGEA
                     GALMDFFKMRSDDLVRMCKKAISLKKR"
     gene            complement(9722..10879)
                     /gene="metK"
                     /locus_tag="PLG78_03215"
     CDS             complement(9722..10879)
                     /gene="metK"
                     /locus_tag="PLG78_03215"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNE22148.1"
                     /translation="MFLKDFLFTSESVSEGHPDKICDQISDAVLDAHLAQDPASRVAC
                     ESLVTTDFVCVAGEITSNAKVDVEALARKVINKIGYTTNDIGFCADTAQVMVRLHSQS
                     PDISMGVTEGEGLYKEQGAGDQGLMFGFANDETPELMPLPISLAHRLMKKLADLRHSG
                     EFKDILPDSKSQVTVEYRDGKPSRIDTVVISTQHREHLEHKVLTEMLVEQVIKKVIPA
                     NYLTNTRYLINPTGKFVVGGPHGDCGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDR
                     SAAYMARYVAKNVVAAGLCTKCEVQVSYAIGFPQPTSILVDSFGTGKIPDEKIAERVK
                     SVFNFSPGAIEKLLKLRDKGRTYLDTAAYGHFGRDGETFTWEKTDKAEALK"
     gene            complement(10996..11847)
                     /locus_tag="PLG78_03220"
     CDS             complement(10996..11847)
                     /locus_tag="PLG78_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNE22149.1"
                     /translation="MRSLLKCLLPLVLAVVAGGLSAETRRIRIVTEPTGADVYVDGVL
                     AGKTPLDVETDGRLKNIRIVSEGFREISFPLRADGGAELVFGLRKAMQTVPDERSSGG
                     LNFLAMARSAILPGWGQYSKGDGSAFLFGAGTIFAGGLLYQTHLDVDVRRRAAVNGFL
                     RGDYYSLAYYQAQALTNSNLGPTFNASAVILASDLMPSRQDTLINSCVPYSTFQMFTV
                     SKGACDRFHRARRNYHRAKYAFAGMYLWNVLDALIADPRSSVSFSSSPVLSDEEKGVY
                     LGAVVRF"
     gene            complement(11844..>12053)
                     /locus_tag="PLG78_03225"
     CDS             complement(11844..>12053)
                     /locus_tag="PLG78_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22150.1"
                     /translation="HYRQAKSIHRNALGAFAILYLWNAVDAFLSAPSFPRETKSLSLF
                     ASPVLAMSERGIERGIQFGATVRFQ"
BASE COUNT         2956 a         3152 c         2999 g         2946 t
ORIGIN      
        1 aactgccgac atcctgcttg taaggcaggc gctctaccag ctgagctaat cgcccaaaag
       61 gaaaactatc aggctgactt cttctgcctg ttgagtagag ccgccatgcg gctctttctg
      121 cgatctgcct ggcgatggtg gatcaaattc ttttgacctg cgcgagccag gaactgggtg
      181 taagatttga acgcagttgc agcatcttcc gtcttgcctt cagccaccag agtgcgaatc
      241 ttcttatcca gtgtccgcag acgagcgcgg gattgagagt tttgctcgtt acgtttacga
      301 gttttacgaa tgtctttctt ggatgctctt aagtttgcca attacttaca cctgaacggc
      361 cagaaaattg ttcaggggga gacggtccag cagtttttta tcctccttct tctttgccac
      421 gattcgccgg ccggcctgaa gaatggcatc gccttgcacc gacaatagat ggaacggacc
      481 atgctctggc aactgacgaa cggaactctg cttcttcctg atgggccccg aaagctctcc
      541 ggcctgcttg tgtccggagg aagaatcgaa gaggtgctcg ctccaggcga tgaccagccg
      601 gacatactca ccctcaacct tcacggagct ctggtaattc cagggctcat caacagccac
      661 gacaccctga tggccaccta tctaccgacg aagggagaaa actggccaca cttgaactgg
      721 ctctccttcg atatagagac aaagcgatcc gctctattcc gcgaaaggat gctcctggac
      781 gtggagaccc tgtatagaat ggggagcctg aagaacctcg agtccggggt gacggccgta
      841 gtcgaccatg tgccagactt tgttcgcaat ccattcctga gcagcatgag cgtggagatc
      901 ctacctgatt ttggaatcgc tcacagcgtt tgctcgtact ctcttggctg gggtcaaggg
      961 ctcgaggcag aacacaagcg agcagtagaa aacgacctgc ctttcatcct gcacatcgca
     1021 gagggattcg atccggaaag cgaattgtcc ctgcgcgtgc tcgatcgagc tggctgcctg
     1081 agcgaacaca cggtcctggt gcacggcctt tctttgtcca acgatgacct ggatcgcatc
     1141 gcagaggcag gcgcacattt tgtgtggtgt ccgtccgcga acctttttgc atacaatcga
     1201 accgtcaaaa tctcagaatt cctgaccaga ggcatcccgg tatgcctggg cacggatgcg
     1261 gctgtaactg gttcgaccca catgctttct gaaatccaaa ctgcgctgaa agtctacaaa
     1321 gaccagaccg gggaagatct gcccgattca gatctgttcg ccatgctcac gaccaacccc
     1381 gccagggcct ttcgctgggc gggcagagga agcctcaagc cggactctct ggctgacttt
     1441 gtggtgctgt cctgtccttc tttgcgagaa gcaaagccag aagatatcta cctggtggtc
     1501 cgcggaggcc agccggtata cggaagcgag gacctggaac cgctgtttat ttctgctgcg
     1561 gtgcatgtgg aacgtgtgga cattggcggc actcgaaaaa ttgtccaaaa aggggtgaag
     1621 gatttgcggg aaacagtcag gcaggctaca ggacgagaaa gtttcccttt cctctccggc
     1681 tggtgatatg ccaatagctc gaaattacaa cggcggctcc atcgtctact tcgccggaga
     1741 caaaggcgac gaagtctacg tactgcaggc cggccgtgtc atactcatca gcacagccct
     1801 ggatacaggg gaagaaatcc gcgaagacgt acagatcggt gagttcttcg gcgtaaaatc
     1861 ctccctgggt aagttcccgc gtgaagagac tgcgcaagta attggcaaga ccacatgcat
     1921 tgttttcaag ctgcacgagt tcgagcagct tgtgatgaag aatacgcacc tcattttgaa
     1981 aatgctgcgc gtattttcca agcagcttcg caacatccac agacaaatcc gggacatact
     2041 caaagcgggt gccgcgaaag atccgtcgtt cgaactcatg agtgtagcag aaagtttcta
     2101 tcgatccggg aatgtggatc atgcagtcta tgcacttgaa aaatacttag aggtctacgc
     2161 ggacggagat tacacggacc gcgcgcacga tctattgtcg caggcccgca agggcctgac
     2221 gtatccacac ggttatcctc cactccagat tgcggatcaa aaacacaaag tggatgcaag
     2281 ctttgtcacg cgcagcatgg cgtccgcaga tagcctcgga gatgatcctt tcgcgctcga
     2341 agaaggagcg ccggccgcac gacctgcgga aaaagaaccg ggcgctgcag acatcctcat
     2401 ggatggactc acattgatga gtcagaataa ggcagacgcg gctcttgcca aattcaatga
     2461 atgcctcgct atcaagaagc ttcacactgc ggacgatcag gaagcagcag aaagagcaca
     2521 gtatgaaaag ggacgcgcgg aactaaagct gggcaaagta gacgatgcat ccaacagctt
     2581 ctccatttat ttaaagaagt ttccaaccgg cgaattcatg aaacacgcga tcttccagct
     2641 tggagttgcg gcagaagcaa agggcaacaa agaaagagcg cgcactcttt atcacaaagt
     2701 ggccaccatg cctcctccgg atgatgcgac gcgcgacgca agaaagcgcc tggagaaact
     2761 gggctgatgc ttcttccaga atccttattc gataagtttg gcaaggtgtt cgaacccggc
     2821 cagatcattt tctgcgagta tgaacccgga aatgatttct actttattca ggaaggtcgc
     2881 gtcaagatca caaagaccat cggcaatgcg cagaagacac tcgatgtgct cagtcaggga
     2941 gacatcttcg gtgaaatggc aattctggaa gaagaaccac gtagcgccac cgccatcgca
     3001 gtagacaaag ttcgcacact caatttcaac cgtgcgaatt ttgacactct catgaagtcc
     3061 aacccacaac ttgccttccg gctgcttgtc attttttcaa atcgcatcaa cgatgcgcgc
     3121 agacgacttc aaatccttct gctcgatgac accacatcga aggtggtgga cgtattcctg
     3181 atgctcgctg aaaaagacgc acactatggt caggtcaagt ccatggtgtt caacacgact
     3241 gtggacgata ttgccagctg gtgtggtcaa cccatcgacg aagtacagaa gatcatcaat
     3301 acatttgttc gtcagggcaa agtagaactt ttcgcggata gaatcgtcgt cagcaatatc
     3361 aacgacttca accggctcat tcaggcgaag agaaagagcc tcttgtaaaa tccagaaaga
     3421 tgaagagatt cccgtacaat ggatgggaat acgtgtaggt ttcctttttt gtttcctgat
     3481 ctatgtaacg attggacatg aatccacgcc tgaatcgatc cattcgcgcc agattatgga
     3541 aaaagtaagg ccgccagccc cagtttctcg agcgaaagtc atcgtctctg gtgaagactg
     3601 cattcgaact tcgcaggaaa ttcgaagacc tttgatttcc aaactgatcg catacataga
     3661 cacgatagca cgcagaagga agacttccca gaaggcgatt gagcgcttcg tccagatccc
     3721 cttcaaacga aatcttcttg agcatttctt ccatgagttc gttcaaatga aactcaagag
     3781 acaagaggct gtagttctcc tgacgctggt tttggatcac tgagtcgagc acggcacgaa
     3841 ttctgtcgcg atatgtatct gcaatttgca aatccggttc aggttgcgaa aagaaatatc
     3901 cctgaatgta gctaccaccc gcatcgatcg agttgactag ttcttcttca aactcaacgc
     3961 cttcgaaaag gacagatatt ccgatctctt gcgcgaaatc agaaatagaa gaaatcagct
     4021 tttgatattc ttttttctct acgctcttct tcaccaggcg aatatctatt ttgactatgt
     4081 ctggcttgag atgaatgagt ctgtcaaagt tgtgaaaagt gaaatcgtcg attgcgatct
     4141 tgcatcctgc ttcgcgatag cgtgaaatca cagaatccag aatcaccagg tctccttcaa
     4201 attcatcttc ggtgatttca atgactacgt tgcgcggatt gagcccgagt tcctcgagaa
     4261 ggcgaagagt tgggatttca gatcctgcgc gaaagacctg catcaaccag gaaggcttca
     4321 cgttgataaa cagcctgccc tgatagttca catctcgcgc atgcagaaat gcaagctctc
     4381 tgactttgcg agatcgaatg agcttttcat gacccggtac cgttgggtcc gcgaaataag
     4441 gacccatact ggtgaagtga cctgactcat ccacgaaacg cccgagcact tcgtacccgt
     4501 aaatggatcc gtctccaatc gagacaatcg gttgaaagaa aggtgcaagt cgataattcg
     4561 aactggcact cgccatcatg tgatagaagt ccagcgcacc acccccgctt agcataattt
     4621 gcattatgca cgtatcgtgc cacgatcatg tcgacaacga tcacactatg tttcacagtg
     4681 ttgcgggcgc gtcgattcca aaaaccaggg aaagaaacta ctctaactgc ataaagtttg
     4741 ggcgacatgt atcgaggcgc atcggtttaa aaaaaattgc ttgagggtat gcatccttgg
     4801 atacccgctg gaggcgtgga attcacacga aaggataggg gcccttgcag tgtattccac
     4861 atcacagggg atgtggatct ctacaattcc gatcgcttaa aagatgcgct gctgcaacat
     4921 ctggactctg caggaaagag catcatcctg gacctgaccc acacggatta tttggactca
     4981 accgggatcg gagcaattgt cgctgcctgg aacaaagcta aagtgatcaa ccagcaggtt
     5041 atgctttcca gcgtaagcga gtccatccgc cgtgttcttt ctgtaatgca gattgatgct
     5101 catattcaga tcttcgaaca cacggacgcg gcccttgctt ctcttcgcag atgaagtttt
     5161 gtgcttcaca aaactgcgct cactttcata aagttcgccg gagcaataca tgagcaaaaa
     5221 ggcgtcctgg aaagagatat tcggctgggc aatgttcgac tttgcgaact cgtcctacac
     5281 taccgtcatc attacagtca tatgggggga tctcttcatt cgcctcgttg tgggagatgg
     5341 gcctgatttt agaaggggaa accttgcatt tggcatcgca ctcttcatca gctatatctt
     5401 ctgcgtcatc accgggccca tcttcggggc catcatggat ttcagtgcgg ccaaaaagaa
     5461 atttctgttt atgagttata tgctcacggt ggtttccacc tgctcgttat tcctggtttc
     5521 gcctggcatg gaagtgctgg gcgtggtact gttgatcatc tccaacttcg gattcgccgc
     5581 cggcgaatca ttcgtggcca gcttccttcc gagccttggt gaaaaggaag aactcgggaa
     5641 aatctccggg attgcctggg gcattgggta tctgggcgga ctcgtctctg tgattctagt
     5701 tggtctactg ggaagtgtag aaatcgagaa ctttgaaagg ctgcgctatg tcggtcctat
     5761 gacagggatt ttcttcatgg tcgccgctat tccaaccttc ctgtggctcc gcgaaccagg
     5821 cgtcgcacac gggcttccgc agggacaaag ttatcttgga gttggattcg cgcgacttcg
     5881 ccagactcta agcgaagtta aagacttccg cgatctgggc atcttctttg tttcactctt
     5941 cttcgcgtcc gccgggctct cgattgtcat cagctttgca ttcacgtacg gagcgcaggt
     6001 gattcactgg acagacgcaa ccaagaccat gatgttcgtg atcactcaat tcacagcact
     6061 gtttggtgca ctcggatttg gttatctgca agataagatt ggtgcaaaga tcacatacat
     6121 gatgactctg gtcctctgga tcattgcaat catctgcatc ttcctcactg attcgatcgc
     6181 ggcatccctc ggagtaaaac cggaacaggc gtttttgatt gtaggatgtc tggcaggtgc
     6241 tggcctgggt gcgacgcagt ctgccggccg cgcactcgtt ggcgtatttt caccggaatc
     6301 aaaggccgga gaattctttg gactgtgggg actgattgga aaactcgcag ctgccattgg
     6361 agtatttggc gtggccgcac tccagacaca gcttggcctt cgcctcgcga ttctattctg
     6421 tgctctgttg tttgtagcgg gactgctgtt cgtaattcct gtgaacgaaa agcgcggcca
     6481 ggctgctgct gaaaaacatg agggcgagta atcgaaccat agcaattgca gccgtgttca
     6541 tcacggctgc agtgaccgcc ttcaaattct tcttcgcaat ccagcttcct ctccatccag
     6601 atgaagcata ctactgggat tggtccaggc atctggactg gtcctactac gatcaaggtc
     6661 cgggagttgc cttttacatc cgaatcttca cttcagttct aggaaacacc ataccggcac
     6721 tgaaactggc ggcgctctct gcggggctca tagcgcacat tctcttgttc ttcgcagcgc
     6781 gacgcgccgg agtttcagcg gcctcgtctc ttttgtttgt atgctgcacc attctggtgc
     6841 ccggcttttt ttccggatca ttcctgatca tgcacgattc agttctcatc cttttctggt
     6901 gcgcggcatt cttgttcatg ctcagatgga tcgaaacaga ttcgtccgca gatctattcg
     6961 ccttatttgc gtgcatggcc ctgggcttcc tcgggaagca caccatggtt ttctttggct
     7021 tatcgtttgt gatctggttt gtgcttactc ctgccagctg gagggagctt cgcaacatgc
     7081 gtctgtatgc tgccattggt tttgccgcaa ttctctcctc ccctttcatc atctggaaca
     7141 tgcagaacaa ttgggacggt ctgggtgcaa tcctcaacct ccgatccagc ggcggagcgt
     7201 tcgcaaataa atccagcacc ggtgcattcg tggtaggtca ggcgttgctg ctttcgcccg
     7261 tatggtttgc tctattcctg ggtttcgctt tctggattct gtttctacaa ttgcgcaata
     7321 gggaaatccc gcgttctttg cgaagcgcga tggaaatgtt tcgcaccgat cgttcccccg
     7381 aagccgcaaa gaaccgattc atatggatca tcgcactgat tctgcccgtc ttcttccttg
     7441 cactttcgcg tcgacgcgaa gttcagccta actggacctt cgcatcttat gctggcatca
     7501 tgctgatctt ctttgtctac agactggagc aaaagaaatg gacgcgggtg attctggcag
     7561 ccggcgccgt tgtagcactc gccatgaaca ctttcttcct cttttcagca cctctcacaa
     7621 aagcattggg cataccacaa aacccaagaa ccgctcgtat tctaccccac ctgcgacttg
     7681 caggatcaaa ggaagtgatc gaagcagtgg atcgccagag acagaaaatc gatccttccg
     7741 ctgggatcct tgcaaacagg tatcaggacg cagcgctggc tggattctat ttagcagggc
     7801 aacctcaagt cgtttccatg aatgtgatgc agaggaatca atatagctac tggccgggac
     7861 cggaaccagg gaagaactat ctggtattca tggtgcagga caatacttgc gaaaaagcaa
     7921 ctttcttttt tcaaccagtt ctttattata tgttcgagaa aatcgaggaa tatccggaat
     7981 ctgaaatcgt actcgatggc gtagccgtta agcgctacca gacctggtac gttcaaaact
     8041 acaaacggga ctggaaagga cttctactct actacatggt agacgcagcc atcctggact
     8101 tcatgcccaa catgcgcggc tacaatcctg ggctgaacag tgcagaagga atggccagta
     8161 ttcagaacta catgaatcag aactacttca tgcgaaaagg agacgtcagt tgcgattgat
     8221 tgctctcata cttgctctgt taacattctg tgcgaagaaa gaaaaacttc cggatccggg
     8281 ggattacagg gcgtttatcc agggaagcgc acacttgctc tgcggcaaga tggttgaatg
     8341 ctattcaggc ctttactttt ccatttcgcc tgaattgagg cgacagatca cggtggaagc
     8401 gtgcgaaaaa gcagcactca gagacctgga ccgcaaactc gcccgccata cccagagcat
     8461 gaaaaatcta tcagtgattt gctatcaggc gattctcgat agcccctgta aagaagtggg
     8521 aaacgccttc ttctggaatc cttcctgctt tgcgcttaga aatgaatcgg aaaaagcgtt
     8581 cgcggacgtg cccatgccca acctgttcgt acttgagaag aagtaaaagc gactaacgtt
     8641 tcttcagaga aattgctttc ttacacatac gcacaagatc gtctgaacgc attttgaaga
     8701 aatccataag tgcgcccgct tccccgctct ggccaaactc gtcctgcatt ccatttcgca
     8761 gcacaacgca tggttgattt tcagaaatca cttccgcaat ggcagaacca agtccgccga
     8821 tcacgttgtg ttcttcggaa gttacaatcg cacctgtctt cttcgcgtag gccagaacca
     8881 gcttctcatc aattggcttg atgctggcca tattgatcac ggcagcctga attccttgct
     8941 tcgccagttt ttccgctgcc aggtctgctt ccgcgaccat cacgccacag gcaatgatcg
     9001 taacatcctt accgtctcgt ctgagctctc cctttccctc ctgaaactta taggaaggtg
     9061 ggcgcgaaag cacaggagtt gccgcgcgcg aaacccgaat ataacatggg cccttcatcg
     9121 cagcaaccgc tgcgatcatc tgcttggcct ccgtgaaatc ggaaggaacg aatacgcgca
     9181 tctttgggat cgcgcgcatg actgcaaaat cttcgatgca ctggtggctt gcaccatcct
     9241 caccaacagt gatgcccccg tgagatgcaa ccagctttac gttcaagccg ggataagcga
     9301 cagagttacg gacaatttcc caggcgcgac cggaaagaaa catagcaaag ctcgaagcga
     9361 atgggaccag tcccgaaaga gaaagcccgg cagccattcc aacgaggttt tgctccgcga
     9421 ctcctacgtt gaaaaaacga tccggatacg cgcgcgcgaa tttgtgagtg cgagtagatt
     9481 cagacagatc tgcgtcaaga accacaacct caggccgagt cttgccgagt tccgcaagtg
     9541 cttctccgta accgtctcga gttgcttttt ttacaacttc aacaacgggc gcagctttcg
     9601 cgctttgccc ttttgcgacg ccagcttttt ttgccggctt cttactggca ggctttcccc
     9661 tggatttcgt tgctacttta acaggtttgc gcgcagcttt tttcttcagg gcagccacag
     9721 attacttcag ggcctctgct ttgtctgtct tttcccaggt aaaggtttcg ccgtcgcgac
     9781 caaagtgacc ataggctgct gtgtccagat acgtacgtcc tttgtcgcga agcttcagaa
     9841 gcttttcgat ggcacctgga gagaaattga atacggactt tacgcgctcc gcaatcttct
     9901 catcaggaat cttgcctgtg ccaaaagaat ctacaagaat ggaagttggc tgaggaaatc
     9961 caatggcgta ggaaacttga acttcgcatt ttgtgcaaag accagccgcc accacgtttt
    10021 tggccacgta gcgcgccatg taggcggcgc tgcggtctac ttttgttgga tcttttccgg
    10081 agaaagcgcc accaccgtga cgaccatacc caccgtaggt atctacaatg atcttacgac
    10141 cggtcaaacc acagtctccg tgaggaccac cgactacgaa cttaccagtc gggttgatga
    10201 ggtagcgagt gttagtcagg tagtttgcag gaatcacttt cttgatcact tgctccacga
    10261 gcatttctgt aagcaccttg tgctccaggt gttcgcggtg ctgagtagaa atgaccacag
    10321 tatcaatgcg agaaggtttc ccgtcgcgat attcaacagt gacctgagat ttggagtctg
    10381 gcaaaatgtc tttgaattcg cctgaatggc gaagatccgc gagcttcttc atgagtcggt
    10441 gagcaagaga gatgggcaga ggcataagct ctggcgtttc atcgtttgca aatccgaaca
    10501 tcagaccctg gtcgccggct ccctgttctt tgtagagacc ttccccttcc gtaacaccca
    10561 tagaaatgtc aggcgattga gaatgcaggc gaaccatcac ttgcgctgta tctgcgcaaa
    10621 aaccaatatc gtttgtcgtg taaccgattt tgttgatcac tttgcgcgcg agggcttcta
    10681 cgtccacttt ggcatttgac gtaatttcac ctgcaacaca cacgaagtct gtagtaacaa
    10741 gactttcgca agctacgcgg cttgccggat cttgcgcaag atgcgcatcg agtactgcgt
    10801 cagaaatctg gtcacagatc ttgtctggat gcccttctga tacggactca gaggtgaaga
    10861 gaaagtcttt caaaaagatg gccatgggtt ctcctgatag accgcttcaa gcggaatgat
    10921 cagctttttg ggagcggccg gtgggtcaat gaaaagagca ttgaggaaac gtgcggccat
    10981 ttccctgcga tgccctcaaa aacgaacgac tgctcccaga tagacccctt tttcctcatc
    11041 cgaaaggacc gggctgctcg aaaaggagac gctggacctg gggtccgcaa tcaaagcgtc
    11101 tagaacattc catagataca tgccggcaaa ggcatacttt gcccggtgat agtttcgcct
    11161 ggctctatga aagcgatcgc aggctccctt ggataccgtg aacatttgaa acgtcgagta
    11221 aggtacgcaa ctattgatca aggtgtcctg ccgggacggc attaaatcag acgcgaggat
    11281 gaccgcgctg gcgttaaagg tcgggccgag attgctgttg gtgagcgcct gcgcctggta
    11341 gtatgccagt gaataatagt cccctctgag aaagccattg accgcggctc ttcgccgcac
    11401 atccacatca agatgcgtct gatacaacag accgccggcg aagatcgtgc cggcaccgaa
    11461 caaaaacgca gagccgtccc ctttagagta ctgaccccat ccgggcagaa tggcagagcg
    11521 cgccatggcc agaaaattca ggccgccgga cgatctctca tccggtacag tctgcattgc
    11581 ctttcgaagt ccaaatacaa gctctgcgcc accatctgct cgtaaaggaa atgaaatttc
    11641 tctaaaacct tcggacacga tgcgtatgtt tttcagtctt ccgtccgttt caacatccag
    11701 tggtgtcttt cccgcaagca caccgtccac atacacatcc gcacccgtcg gttctgtaac
    11761 aatacgaatg cggcgcgttt ctgccgagag accgcctgcg acaactgcca ggaccagggg
    11821 aagaagacat ttcaaaagtg atctcattgg aacctcaccg tagcgccaaa ttgaattcca
    11881 cgctcaattc cgcgttcaga catggcgagg accggggaag caaacagaga aaggcttttg
    11941 gtttcgcgag gaaacgaagg agcactcaaa aatgcatcga ccgcattcca caaatacaaa
    12001 atggcaaacg cgcccagtgc gtttctgtga atgctctttg cctgcctgta atg
//
LOCUS       DAOQJL010000043        12043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2650_length_12043_cov_4.026610, whole genome shotgun sequence.
ACCESSION   DAOQJL010000043 DAOQJL010000000
VERSION     DAOQJL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_12043_cov_4.026610"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLG78_03230"
     CDS             <1..717
                     /locus_tag="PLG78_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22151.1"
                     /translation="QRILMLLILTLPLACKDGETIEDIGSYSITTKDFENHYSVLVEK
                     VAQLRNGDKSTFARLMCHPERMPDDPYLREVVRNLEPKEFYERYREMRIIEQAARKEN
                     FQDDPIVKRMMEQAQLEVLTSLFVMKKLEKRIKISDEESAAKCVELRQKLPDQLGPLT
                     LDECKKIAQGFIREERMRTEMPRVREEIKESVTISKNQKFDRDDFLANKLETYKVLKK
                     TGGCDADSAGPALETPAKPK"
     gene            complement(725..1849)
                     /locus_tag="PLG78_03235"
     CDS             complement(725..1849)
                     /locus_tag="PLG78_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE22152.1"
                     /translation="MKGTLYFDYNATHPPIQEILKECLEIYVSNPANSSGISALSQEN
                     QRRIESSREQIAGSLSTKSTQKILPSNLTFVSTGTEALHQMVRTYARPQTRALLSPYE
                     HEAMIAACKEANLSIDMIRADKSGVVDPDEVNRLITPEHSFVSVLAVSNETGAIQPVS
                     SISEITRRKGIPFLCDCIQAAGKLEIDYALFDAFCINGHKFGAGFGTAVLASRKEPVA
                     LFGGGLQEDERRAGTENLFSILCTASALEMQTALLAEKNERLRRFQKKIEESILEKTS
                     GELTAAGSPRATNTSYFVFPELENIDFLFLGLDQKKVVVSTGSSCKSRTRQPSRSLQA
                     MGYSKQDSMRAVRISTGLFTTEQEVDEFLAAFFYSIQAAS"
     gene            complement(1846..3048)
                     /locus_tag="PLG78_03240"
     CDS             complement(1846..3048)
                     /locus_tag="PLG78_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22153.1"
                     /translation="MLKLLARQIARHWKGLLPVLLLWIAGGYYIFHNKHSILTSLAHF
                     AVKEPAAGEQFPEKARPFVEHAREETHGIRLDLMEKACVQIPLRYRGESELFQPHWLE
                     RYEKWNLQTNREIPPRTDDVVEPDSYWKENRPHVIRALKDTLAASVFAFEIPPAEGSK
                     EGINVADETAKYARAACMDPIIVFMYGDYAEHLEARARLKIVKADKDFELNHPSMQDQ
                     ELYILESLKNSDPYKRALASYMGGPVPITEKACTGREYRLACVAPEEAAIVMRKRIYV
                     ADSAELPALFINLAKLHMLGAGEDHKARALDYFYGGIREISTEKEARAGLARAYMALD
                     KVDRAYEQIAQASLLYRDHGRTDNEFRELARSVLMKAGKFREADCYSDYSDLPSGERD
                     YCVGFKLQ"
     gene            3218..5131
                     /locus_tag="PLG78_03245"
     CDS             3218..5131
                     /locus_tag="PLG78_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22154.1"
                     /translation="MKRALLVGLVFASGLASQTMPVIPPQPDLLLRAAYDAEIKEDRE
                     GALALYEKYLSQGEESAFVRGQESRLYAALGRKQDAVRLAKKAIDLAPDRKEYYVLYA
                     ELLRQYGRSEEAYKLLRDAVLKFQDDSEVEFFLAEACNETGRISESILHYHQTLYYPA
                     TGSTRYPVYRSVALLRLAVYKLRRQEREAARKYLTRYLDLNPDRLYPRYLLATDVLFD
                     MGDFEGAMREMEFVWHSDRSLYKDAAIEEPRLRGALALVHFYFDTPEAEGFLAASARD
                     NPGLFPALLATQQKKDQEAAALLLPILKANPAQLFGRIALYRILSRRGGELTGERMSL
                     AAILMQNNRVRDGIALLEESAAEAKSGKTQIPLWKIERMIAQSYLKLKQPYRALLSQR
                     DAMAGVPQDSPSMAEMQLELAKILSGNPINRDQEAVSILDGLIANGNENALYHRALIH
                     QSLGACQDANRDWLEIERMKGPQMEAAFLRGGCAESMGDFAEAEKAFREVYSKRSDLP
                     LIQNSLAYVLSLQGKELQEARSLIEKAVAADSDDAHFQDTYGWIYFQTGDHLRARYHL
                     QFASRLLDEEGDRNAEVFDHLGHVYEALGQNERSLYYFDSAYRILAEKAKRMPFEENL
                     MKNIQSKMEKQKK"
     gene            5128..5877
                     /locus_tag="PLG78_03250"
     CDS             5128..5877
                     /locus_tag="PLG78_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22155.1"
                     /translation="MNKALILAFCSFIPGLGCKTPQTQELPVQELSVRDRLEKIRAAY
                     APAPCFYASFGVESFQPGKSGQKADGTVRVDNANRRVRFYFTDSIFGITLSHMTIRDG
                     FVYIGSPRDPVQQIPVDRFVVGGLANNSIRLPFRLFEDLMYGRVPEQVFADKTHYRTE
                     GARLFAEYEDRESKSVYEFENDRMRSVTYLHKQDRANAKAEMTGTYPRSPFPQKMELN
                     GWIDGPPEKMIVNFQGVDMTAVCKEVQFPVN"
     gene            complement(5823..7109)
                     /locus_tag="PLG78_03255"
     CDS             complement(5823..7109)
                     /locus_tag="PLG78_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE22156.1"
                     /translation="MQIARKYSLSARTAACAIFCASFSVSYTASFLGLSLFFRIVEWR
                     KPQGKIPPPFLAAILMYAWLLLDWCIHLYMGEANLHNLVRGETGDVFLFLFGLHVYFL
                     LDDDRHRIIILKGFTLFAIVIVISGALSVFSEQRLARAIYGKGFLATSITTPQHLFAV
                     VRGIAIYRPIGFMNTRLTYAGILIMVIPFVFHFAREATGRKNQMGWIALLVLSLAVLV
                     ANGTRSAQIGIAFALCVSAAEFLFRTNRRLFFAAAIAATMLISLVYLYGDFSGRQTDF
                     QRPILWTGAGAIFENHPWIGTGPGGFASAILEWKKTFNLSHPMSWYYVESTPSGHAHS
                     DLLHLSAIGGIPGCLFFLSIVALSVRTVASQKSEAILSRSALIAFFAAGVAQCYFQDD
                     EVVVIYWVLLAFLERMRSDQFTGNWTSLQTAVISTP"
     gene            complement(7114..7872)
                     /locus_tag="PLG78_03260"
     CDS             complement(7114..7872)
                     /locus_tag="PLG78_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22157.1"
                     /translation="MRVLMLILLSWIVSCQSAPIAPGVAKGNGKPSIPAASRAAVFTI
                     LVREVSVSRSEGAPWFSQLTDDDAQKVKAKLKEDLERVLKFELVPGEKIRAQDLYRAG
                     RFPLHEPYYINPELDPIVRNEAEQELMMRTSNALGAKYFVVIVVDHSTTKVLLKPASV
                     TSSIQIQIFSPGSGLIYTATEEESADALPYVRDVPDAEFKLRYRGIMRSTALDLIAKA
                     RSKLEKRMQSDITILPRESPATPPAPPAPARVDT"
     gene            7953..8144
                     /gene="thiS"
                     /locus_tag="PLG78_03265"
     CDS             7953..8144
                     /gene="thiS"
                     /locus_tag="PLG78_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNE22158.1"
                     /translation="MIVNGSEINTGATSLVKLLEDLGIRPDTVAIELNGQIAKRDTWK
                     DVLLKEADKIEIIKFVGGG"
     gene            8146..8817
                     /locus_tag="PLG78_03270"
     CDS             8146..8817
                     /locus_tag="PLG78_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNE22159.1"
                     /translation="MLPPVYPILDVSTVRAEKLNELELLESWIRSGLNFVQIRWKESG
                     LAEYVSYGQKLKSHFPALRIVANDHYRAVMERPDVFELVHLGQEDYSRIPELPDRVRF
                     GISTHNARQMEVAIAMFKRGLPGSYIALGPVRPTKSKPTGTDPVLSESELAECLQLFR
                     SSSLDLVLIGGIDSENISSIVYPGFARDYGFVPTVAVIRGALGSGLTPIAERIRQERS
                     GLKDR"
     gene            8849..9682
                     /locus_tag="PLG78_03275"
     CDS             8849..9682
                     /locus_tag="PLG78_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNE22160.1"
                     /translation="MNGVMEPETYSSAREDRDYLLEYKSVDLRSLRRESVLDVPVDNV
                     TRDEAVALLLDLIEKKKGPHAVMFMDPVKLSAIRPGMKNHWIAEQARVILPDGAGMLW
                     ASRMLRHPLKERIPMIAFLMDIVRLSMKKEFTIYLLGSKVDTVEKAFHNLNKSFPGVR
                     IIGRHGGHFDAERGELVRQALRKSSPDVIFLGMGFPTQEKWYRDNIDFLSRSVVVFVD
                     GAFDVLSGNERKAPDWAQVRGLAWFWRTITRPYRVLRFLRMIRFYVSVVFRNLKMRNS
                     G"
     gene            complement(9685..>12043)
                     /locus_tag="PLG78_03280"
     CDS             complement(9685..>12043)
                     /locus_tag="PLG78_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22161.1"
                     /translation="IRARLDEIPPGLKKAIITATVDEKVSRSDQQLIMHMLIDQATPD
                     AIADFLEPRIGYRPDLTPPQYRKDGVQIIYADGMSPEEIAGRRKKVRITIFAVLGGVA
                     ATLIAFGSVLLYNKLSIAGRYKDGLAELSQAALTTGNEREAHRKRAEAFYQKALSSSG
                     TYDPEVMNKYGLAYLRAGYYDDAFEKLFGRVEPAYDWADPAARAPLIRLKDGSKFPSP
                     EQQAKGDRAEFLDRQNVKRKLEVPGAYTVTRLRDGNLDKRTLLSLGKFHSHNAQSFTR
                     RPEGQKYKNDDLGIDYYRLILTLMEMPDDVDARSGIGEVYYNRKEYASAAREYNKIIE
                     KFPNEIKGHAGLLNTYIQIWHENQDPRYVLEKHRLIQRLGLEKDLPIYLLTKLAGFYI
                     DLNEDDVRIRYQVDPVNSVTGLGLEDNATHLLELVFKKEDERDGEIINGASYGEGFYQ
                     RGRFLLKQKETLRALRQFQNAHQHDPRHYLAVREMGAYYRDNLDFARAEEYFKKSLEL
                     YNQYKDDYGAKPEDETLIAGDRGLLFYDMGSLLFLKYAGTPRENKEAFADSRIYPDRG
                     RNAETSETQKRRELLSQARDYFGQALKEEVRDEKAKVQSIYWMGWIDYISSDFESALV
                     EWEGLQDIYSGNYSDPVLLMARANSYYYTDQNRSALGDYLKLQNDLERRALSIDHAVP
                     EDPEHQKVFLTLAALYNNLGAVYEKEFNEAKKRGASQRSLEDLEKNSLKYYWKSIESA
                     RKVEEDNEIARTNLQKAFRSAVKSDPALDDWISPALPGLTEKKKK"
BASE COUNT         2934 a         2880 c         3119 g         3110 t
ORIGIN      
        1 caacgcattc ttatgctgtt gatccttact ctgccgctgg cctgtaaaga tggagagacc
       61 atcgaagata tcggcagcta ctccatcacc acgaaggatt tcgagaacca ttattccgtt
      121 ctcgtggaaa aagtggctca gcttcgcaat ggagacaaaa gcactttcgc gcgtttgatg
      181 tgccatccag aaagaatgcc ggatgatcca tatctgcgtg aagtggttcg caacctcgaa
      241 ccaaaggaat tctacgaacg ctaccgcgaa atgagaatca ttgagcaggc ggcacgcaaa
      301 gaaaacttcc aggatgatcc aatcgtcaag cgtatgatgg aacaggcaca gctagaagtg
      361 ctgaccagcc tctttgtaat gaagaaactg gagaagagaa tcaagatttc agacgaagag
      421 tccgcggcca aatgcgtgga actccgtcag aagcttccag accagcttgg tcctctcaca
      481 ctcgacgaat gcaaaaagat cgcacaaggt tttatccgcg aagagagaat gcgcactgaa
      541 atgcctcgcg ttcgcgaaga gattaaagaa agcgtaacta tttctaaaaa ccagaaattt
      601 gatcgcgatg atttcctcgc aaacaagctg gaaacataca aagtgctcaa gaagactgga
      661 ggctgcgacg cagatagcgc aggtccggcc ctcgaaactc cagcaaaacc aaagtaaaac
      721 tatcttaact ggcggcctga atcgaataga agaaggctgc caggaattca tccacttcct
      781 gttccgttgt aaaaagcccc gtactgatgc ggactgctcg catggaatcc tgtttgctat
      841 atcccattgc ctgtaaactg cgcgaaggtt gtctggttct ggatttacag gagcttccag
      901 tggataccac gactttcttc tgatccagtc caagaaagag aaagtcaatg ttctctagct
      961 ccggaaatac aaagtaactt gtgtttgtag cgcgaggcga tcctgcggcc gtgagctccc
     1021 ctgatgtttt ctccagaatc gattcttcta tcttcttctg gaatcggcga agcctttcat
     1081 tcttttctgc aagtagagct gtttgcattt caagtgcgct cgctgtgcac aggatagaga
     1141 aaagattttc agtacctgcg cgcctttcat cttcctggag tccgccacca aagagagcga
     1201 ccggttcttt tctggacgcg aggaccgcgg tgccaaaccc cgctccgaat ttgtggccat
     1261 tgatgcaaaa ggcgtcaaac agcgcgtagt cgatctcgag ctttcctgct gcctggatgc
     1321 agtcgcaaag aaaaggaatc cctttgcgtc gcgtgatttc agagatgctc gatacgggct
     1381 ggatggcacc tgtttcgtta gacaccgcaa gaactgatac gaaagagtgc tcgggtgtga
     1441 tgagtcgatt cacttcatcc ggatccacta ctccggactt atccgcgcga atcatatcaa
     1501 tggaaagatt ggcctctttg cacgctgcaa tcattgcttc gtgctcgtaa ggagatagaa
     1561 gtgcgcgagt ctgcggtcgc gcgtaggtgc gaaccatttg atggagcgct tctgttccgg
     1621 tggatacaaa cgtcagattg gacggcagga ttttttgcgt ggattttgtg gagagagaac
     1681 ctgcgatttg ctcgcgcgat gattctattc ttcgctgatt ttcctgagaa agtgctgaaa
     1741 ttccgctgga attggctgga tttgagacat agatttccag gcattctttg agaatttcct
     1801 gaatgggcgg gtgtgttgca ttgtagtcaa agtaaagagt gcctttcatt ggagtttgaa
     1861 tccgacacaa taatctcttt caccgctggg tagatccgaa tagtctgaat agcaatctgc
     1921 ttcgcgaaat tttccagcct tcatcaacac ggacctggca agttcgcgaa attcattgtc
     1981 tgttctaccg tggtctcgat acagaagcga tgcctgtgcg atttgttcgt acgcgcgatc
     2041 tactttgtcg agcgccatat aagctcttgc taaacctgcg cgcgcttctt tctctgttga
     2101 gatttctcta atccctccat agaaataatc gagtgcccgg gccttgtggt cttctcctgc
     2161 accgagcatg tgaagttttg caagattgat gaagagggca ggcaattccg cggagtccgc
     2221 tacatagatt ctctttcgca tcacaatggc tgcttcctct ggcgctacac aggcgagcct
     2281 gtattctctt ccggtgcatg ctttctctgt gattggtacg gggccgccca tgtaggatgc
     2341 gagcgctctt ttatatggat cgctgttttt taacgattct agaatgtaga gttcctggtc
     2401 ttgcatggaa ggatggttga gttcgaagtc cttgtctgct ttgacgattt tcaggcgagc
     2461 acgagcttct agatgttctg cgtaatcgcc atacataaaa acgatgattg gatccatgca
     2521 ggcggcgcgc gcatactttg ctgtttcatc tgctacgttg attccctctt ttgatccttc
     2581 tgcaggagga atctcaaatg cgaacacgga tgcggccaga gtgtctttga gcgcgcgaat
     2641 gacgtgcggc ctattttctt tccagtaact gtccggttct accacatcat ccgtgcgagg
     2701 tggaatttcg cgattcgttt gcaggttcca cttctcatat ctttcgagcc agtgcggttg
     2761 aaatagttcc gactccccgc ggtatcgcaa agggatctgg acgcatgctt tctccatcaa
     2821 gtccaggcga atgccgtggg tctcttcccg ggcgtgctcc acaaagggtc tagctttttc
     2881 tggaaactgt tcccccgcag ccggctcttt gactgcgaaa tgggcgaggg atgtcaaaat
     2941 agagtgctta ttgtggaata tgtagtagcc gcctgcgatc cacagcaaga gaaccggaag
     3001 cagtcctttc cagtgtcgcg cgatttgtct ggcgagtagt ttgagcactg gagttcctta
     3061 aaaaaaatga ttggaagcga ggccgattct agaaatactt tccagcaatg gcacattcca
     3121 agaccaaaaa tgtaaataaa aaacatcgca aaaacatccg ccgcatgaag gaaaagagag
     3181 cggcttctct cctgaagaaa aagaagaagg gttgaatttg aaacgcgctc tgctcgtcgg
     3241 cctggtcttc gcatcgggtc tggcgagtca gactatgccc gtgatcccgc cgcagccgga
     3301 tttgctgctg cgcgccgcct atgatgcaga aatcaaggag gatcgggaag gtgccctggc
     3361 cctgtacgaa aagtatctct cgcaagggga ggagagcgct tttgttcgcg gtcaggaatc
     3421 caggctctac gccgctctgg gccggaagca agacgcagtg cgtctcgcaa aaaaagcaat
     3481 cgatcttgca cccgatcgca aagagtacta cgttctctac gctgaacttc tgcgtcaata
     3541 cggcagaagt gaagaagcat ataagctcct gagagatgcc gttcttaaat tccaggatga
     3601 ttcagaagtt gaattctttc ttgcggaagc gtgcaatgaa actggtagaa tcagcgaatc
     3661 gattctgcac tatcatcaga ctctctatta tccggccaca ggaagtactc gttatcccgt
     3721 ctatagaagt gtggcgttgc ttcgtcttgc agtttacaaa ctgagacggc aagagcgcga
     3781 agccgcgagg aaatacctga cccgttatct ggatttgaat ccggatcggc tctatccaag
     3841 gtacctgctg gcaacggacg ttctttttga tatgggcgat ttcgaaggcg cgatgcgcga
     3901 aatggaattc gtctggcact ctgacagaag tctgtacaaa gacgcggcca tcgaagaacc
     3961 aagacttcgc ggcgctcttg cactcgttca tttttacttt gatacaccgg aagcagaagg
     4021 ttttcttgcg gcttcagcgc gggacaaccc cggccttttt cccgcgcttc ttgcaaccca
     4081 gcagaaaaaa gatcaggaag cagcagctct gcttctgccc attctaaaag caaatccggc
     4141 acagctattt ggaagaattg ctttgtatcg cattctttcg cgcaggggag gagaattgac
     4201 aggagagaga atgtctctgg cagccatcct gatgcagaat aaccgggtga gagatgggat
     4261 tgcacttctg gaagaatctg ctgcggaagc aaaatccgga aaaactcaaa ttcctttgtg
     4321 gaagattgag agaatgatcg cgcaatcgta tttgaaattg aagcaacctt atcgtgcact
     4381 tttatcacag cgcgatgcaa tggctggtgt tccgcaagat tctccttcta tggcggagat
     4441 gcaactggag cttgcgaaga tattgtctgg caatccaatc aatcgagatc aggaagcagt
     4501 cagtatcctg gatgggttga tcgcgaatgg aaatgaaaac gctctttacc accgggcttt
     4561 gattcatcaa tctctgggtg cctgtcagga tgcaaatcgc gactggctgg aaattgaacg
     4621 catgaaagga ccacaaatgg aggccgcatt tttacgcggc ggctgcgcag aatccatggg
     4681 agattttgct gaggcagaga aggcctttcg agaggtttac tccaaaagat cagatcttcc
     4741 gctgattcag aattcgctcg catacgttct cagtctacag ggcaaagaat tgcaggaagc
     4801 cagatccctg atcgaaaaag cggtggcagc agattcggac gacgcgcatt tccaggatac
     4861 atacggctgg atttactttc agactggaga tcatctgcgc gcacgctacc atctgcaatt
     4921 cgcctcccgg ttgctggatg aagagggaga tcgcaacgcg gaagtatttg atcaccttgg
     4981 tcatgtgtat gaagctctgg gccaaaacga aaggtccctg tactattttg acagcgccta
     5041 tcgcattctg gcggaaaaag caaaacgaat gccgttcgaa gagaatctaa tgaagaacat
     5101 tcagtctaaa atggagaaac aaaaaaagtg aacaaagctc tcatcctggc attctgtagt
     5161 ttcattcctg gtctgggatg caagactcct caaacacagg aacttcctgt ccaggagctc
     5221 tccgttcgcg atcggctgga gaagattcgc gcagcatacg cacccgcacc ttgcttctat
     5281 gccagcttcg gagtggagtc ctttcagcct ggcaaatccg ggcagaaggc ggacggtaca
     5341 gttcgcgtgg acaacgccaa caggcgcgta cgtttttatt ttacagacag catattcgga
     5401 atcacccttt cgcacatgac cattcgcgat ggctttgtat acattggtag tccaagagat
     5461 ccagtgcagc aaatcccagt ggatcgattt gtggtgggag gacttgcgaa caattctatt
     5521 cgtcttccat tccgtttgtt tgaagatctc atgtatggtc gtgtgcccga gcaggtgttt
     5581 gcggacaaga ctcactatcg cacggaaggc gccagattgt ttgcagaata cgaagatcgc
     5641 gaaagcaaat ccgtttacga gtttgaaaat gatcgcatgc gttcagtgac ttatctgcac
     5701 aagcaggatc gtgcgaatgc gaaagcagag atgacaggca cctacccccg gtcccctttt
     5761 cctcagaaga tggaattgaa tggttggata gatgggccac ctgagaagat gatcgtaaat
     5821 tttcagggcg tagatatgac tgcggtttgc aaagaagtcc agttccctgt gaattgatcg
     5881 ctcctcattc tttctagaaa ggcaagcaaa acccagtaga tcacaactac ttcatcgtcc
     5941 tgaaagtagc actgagctac gcctgcagca aagaatgcaa tgagtgcaga gcgtgaaagg
     6001 attgcttctg atttctggga agccacggta cgaacagaca aagctacgat ggacaaaaag
     6061 aaaaggcagc cggggattcc tccgatcgcc gacaggtgaa gtagatcgct gtgcgcatgt
     6121 cctgatggag tactctctac atagtaccag ctcataggat gggagagatt gaacgtcttc
     6181 ttccactcta gaatggcaga agcgaatcct ccaggcccag tgccaatcca gggatggttt
     6241 tcgaaaatgg ccccggctcc ggtccagagg atcggtcttt gaaaatcagt ctgtcttccg
     6301 gaaaagtcac catacagata gaccagagag atgagcatgg ttgcagcaat ggcggccgca
     6361 aaaaacaaac ggcgattggt gcggaacaaa aattcagctg cacttacaca gagtgcgaag
     6421 gcaattccaa tctgcgcgga tcgggtgccg ttggccacca gaactgccag agaaagaaca
     6481 aggagagcga tccagcccat ctggtttttt cttcctgtgg cttctcgtgc gaaatgaaac
     6541 acaaagggaa tgaccatgat caggataccc gcgtaagtta gccgcgtgtt catgaatcca
     6601 atgggtctgt aaatagcgat ccctcgcacc acagcgaaca ggtgttgcgg ggttgtgatg
     6661 gaagtggcca ggaaaccctt tccgtatatg gctcgtgcca gtctttgttc gctgaacacg
     6721 gatagtgcac cagagatcac gatcacaatg gcgaataacg taaatccctt gagtatgata
     6781 atgcgatgtc ggtcatcatc cagtagaaag tatacgtgaa ggccaaacag gaacagaaac
     6841 acatctcccg tttctccgcg aaccaggttg tgcagatttg cctctcccat gtataagtga
     6901 atacaccagt ctaagagaag ccacgcgtac atcaagatcg ccgccaggaa tggcggtgga
     6961 attttccctt gaggttttct ccattctacg attctaaaaa aaagactgag ccccagaaag
     7021 gaggcagtgt aactcacaga aaaagacgcg caaaagatag cgcatgctgc tgtgcgcgcg
     7081 gacaggctgt atttgcgcgc gatctgcata caatcaggtg tctactctgg ctggagcggg
     7141 tggggcaggt ggggtggccg gagattccct gggtaagatt gtgatgtctg actgcattct
     7201 tttttctagc tttgatctgg cttttgcaat gagatcgagc gcagtggatc tcatgattcc
     7261 gcggtagcgc aatttgaatt cggcgtccgg aacatcgcgc acgtagggga gcgcatccgc
     7321 tgactcttcc tctgttgctg tataaatcaa acctgagcca ggactgaata tttgaatttg
     7381 aatggaggaa gtaaccgatg ctggtttcag gagtactttg gtggtgctgt gatctacaac
     7441 aatcaccacg aaatattttg cgcccagtgc attggatgtg cgcatcatga gttcttgctc
     7501 tgcttcgttt cgcacaatcg ggtcgagctc ggggttgatg tagtatggtt cgtgaagtgg
     7561 aaatcttcct gccctgtaga ggtcctgtgc ccgaattttc tctccgggca caagttcaaa
     7621 cttgagcacc ctttccagat cctcttttaa ctttgctttc actttctgag cgtcatcatc
     7681 cgtgagctgg gaaaaccatg gcgctccttc tgatctgctt acggacactt cgcgcaccag
     7741 aatggtgaat acggccgcgc gtgatgcggc tggaatggaa ggcttcccat ttcctttcgc
     7801 aactccaggt gcgatcggtg cgctctggca cgaaacaatc caggaaagca aaatcaacat
     7861 gagtacgcgc ataggttgaa cctgaaaaaa ccggcgcgcc cgtcgacgac ttctgttgct
     7921 tgacagcctc tgtttttatt ttttgtggag gtatgattgt aaatggcagc gagataaaca
     7981 cgggtgccac cagtctggtg aagcttctag aggatctggg cattcggccg gatacagttg
     8041 ccattgaact gaacggacag atcgcgaaga gagacacctg gaaagatgtg cttctaaaag
     8101 aggcagataa aatcgaaatc atcaagttcg ttggaggagg atgaagtgct tcctccggtc
     8161 tatccgatcc tcgatgtaag cacagttcgc gcagagaaac tgaacgaact cgaattgctc
     8221 gaaagctgga ttcgcagcgg cctgaatttc gtgcagatca gatggaagga gtcgggcctc
     8281 gcagagtatg tatcctacgg acaaaaactc aaatcacatt ttcctgctct gcgcattgta
     8341 gccaacgatc actaccgcgc agtgatggaa agaccggatg tttttgagct ggttcatctg
     8401 ggtcaggaag actactcgcg aatcccagaa ttgcctgacc gggtccgttt tggaatctcc
     8461 acacacaatg caaggcagat ggaagtggca atcgccatgt ttaagagagg cttgcctggt
     8521 tcctacattg cactcgggcc cgttcgtcca acaaagtcca aacctaccgg aacggatcct
     8581 gtcctgagtg aatctgaact ggcggaatgc ctgcagctgt ttcgatcgtc ctctcttgat
     8641 ctggttctga tcggtggaat cgactcggag aacatttctt ctattgttta ccctggtttt
     8701 gcaagagact acggttttgt tcccacagta gcagtcatcc ggggtgccct gggttctggc
     8761 ctgactccca tagcagaaag gatccgccag gagagatcgg ggctgaaaga tcgttgactg
     8821 gatgggtgcg cgacataatc tgattcccat gaacggcgtg atggagccag aaacatattc
     8881 gtctgcccgg gaagaccggg actaccttct ggaatacaaa tcggtcgatc tgcgcagtct
     8941 cagacgcgaa agcgttctgg acgttccagt agataacgtc actcgcgacg aagcagtggc
     9001 acttctcctg gacctcattg aaaaaaagaa aggtcctcac gcagtcatgt tcatggatcc
     9061 agtgaaactt tctgcgatcc gtcctggaat gaagaatcat tggatcgcag agcaggccag
     9121 agtgatcctg ccggacggcg ctggcatgct ctgggcttcg agaatgcttc gtcatcctct
     9181 caaagaaaga attccaatga tcgcgtttct catggatatc gttcgtcttt ccatgaagaa
     9241 ggaattcacg atctatttgc ttggttccaa agtagataca gttgaaaaag catttcacaa
     9301 tttgaacaag agctttcctg gagttcgaat catcggacgc cacggtgggc attttgacgc
     9361 ggagcgtgga gagctggtgc gccaggctct gcgtaagtcg tcgcctgacg ttatttttct
     9421 tgggatgggt tttcccactc aagagaaatg gtatcgagac aacattgact tcttaagccg
     9481 ttctgtagtt gtcttcgtgg atggagcttt tgatgttctt tcgggtaacg agcgtaaggc
     9541 gccggattgg gcgcaggtgc gcgggcttgc ctggttctgg agaaccatca ccaggccata
     9601 cagagtcctt cgcttcctga ggatgattcg tttctacgtt tccgtggtct tcagaaatct
     9661 gaagatgcga aacagcggct gatcttattt ctttttcttc tcagtaagac ctgggagggc
     9721 aggcgaaatc cagtcgtcga gagccgggtc tgatttcaca gcagacctga aagccttctg
     9781 caaattcgtg cgcgcaattt cattgtcttc ttctactttg cgcgccgatt caatcgactt
     9841 ccagtagtac ttcagagaat tcttctccag atcttccagg cttctctgag atgcgccgcg
     9901 ttttttcgct tcattgaatt ccttttcata aacagcaccc agattgttgt acagggccgc
     9961 gagagtcagg aatactttct ggtgctctgg atcttctggc accgcgtgat caatggatag
    10021 agcgcgtctt tccagatcgt tttgcagttt cagatagtct ccgagagcag aacgattttg
    10081 atccgtgtag tagtaagaat ttgcgcgagc catgagcaat acagggtcag agtagttgcc
    10141 agaatatata tcctggaggc cttcccattc aacgagcgcg ctttcaaagt cagaggaaat
    10201 gtagtcaatc cagcccatcc agtaaatgga ctgcactttt gccttttcgt cgcggacctc
    10261 ttctttgaga gcttgaccga agtagtcgcg agcctgagac aacagctctc gcctcttctg
    10321 agtttctgat gtttccgcgt tgcgcccgcg atcaggatag atgcgcgaat ctgcgaatgc
    10381 ttccttgttt tctctgggcg ttcccgcata tttcaggaag aggagggagc ccatgtcgta
    10441 gaacagtagc ccacggtctc cggctatgag cgtttcgtct tccggttttg cgccgtagtc
    10501 atctttgtat tgattgtaga gctccagaga tttcttgaaa tattcctctg ctcttgcgaa
    10561 atccaggtta tctctatagt atgcgcccat ttcgcgcacg gcaagatagt ggcgtgggtc
    10621 gtgctgatgc gcattctgga actgacgcag agcgcgaaga gtttctttct gtttcaggag
    10681 gaagcggccg cgttgataga atccttcgcc gtagctggct ccgttgatga tttctccgtc
    10741 tctttcgtct tctttcttga aaacaagttc gagtagatga gttgcattgt cttctagacc
    10801 cagtcccgtc acagagttca ctggatctac ctgataacga atgcgaacgt catcttcatt
    10861 cagatcaatg tagaatcccg caagctttgt gagcaggtag atgggtagat ctttttcaag
    10921 tcccagcctt tgaatgagac gatgtttttc gagtacgtat cgcggatcct ggttttcgtg
    10981 ccagatctgg atgtaagtgt tgagcaggcc cgcgtgtcct ttgatttcgt ttggaaactt
    11041 ctctatgatc ttgttgtatt cacgcgcagc agatgcgtac tcttttctat tgtaatagac
    11101 ttcaccaatg cctgagcgtg catccacgtc atccggcatt tccatgagtg tgagaatcaa
    11161 acgatagtag tcgattccca ggtcgtcgtt cttgtacttc tgtccttctg gtcttcgagt
    11221 aaagctctgc gcattgtgtg aatgaaactt accaagagaa agtaatgtgc gcttgtcgag
    11281 gttgccgtct ctcagacgag tgactgtgta ggcaccggga acttccagtt tgcgttttac
    11341 gttctgtcta tcgaggaatt cggctctgtc tccctttgcc tgttgctcgg gggagggaaa
    11401 cttgcttcca tctttgagtc ggatgagagg cgcgcgtgca gctggatcgg cccagtcgta
    11461 ggctggctcc acgcgtccaa atagtttttc gaaagcatca tcgtagtatc cggcacgcag
    11521 ataggccagg ccgtacttgt tcattacttc cggatcataa gttccgctcg aagaaagtgc
    11581 tttctggtag aatgcttccg cgcgctttct gtgcgcttct ctttcattcc ctgtagtcag
    11641 cgctgcctgg gagagttctg ccaggccgtc tttgtatctg cccgcgatgg aaagcttgtt
    11701 gtagagcaga acagaaccaa aggcaatcaa cgtggcagca actccaccta acactgcgaa
    11761 gatggtaatc ctgactttct ttctgcgccc ggcgatttct tccggactca tgccatccgc
    11821 gtagatgatc tgtacgccgt cctttctgta ctggggcgga gtcagatccg gacgatatcc
    11881 gattcttggt tcgaggaaat ctgcgattgc atcaggcgtt gcctgatcga tcagcatatg
    11941 catgatgagt tgctgatcgc ttctcgatac tttttcgtct acagtggcgg tgataattgc
    12001 ttttttgagg ccaggcggaa tctcatccag gcgtgcccga att
//
LOCUS       DAOQJL010000044        11920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2681_length_11920_cov_4.740160, whole genome shotgun sequence.
ACCESSION   DAOQJL010000044 DAOQJL010000000
VERSION     DAOQJL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11920
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_11920_cov_4.740160"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLG78_03285"
     CDS             <1..330
                     /locus_tag="PLG78_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE22162.1"
                     /translation="VKHNQSPQWTMERQDAIELFGILLDNACRFCTGQVVIGLDTSEV
                     TIEDDGPGCTEEQMKQLSLRGARIDEFTEGGLGLSIARELAESYQMTLSFSRSPSGGL
                     RVSIRNS"
     gene            372..1739
                     /locus_tag="PLG78_03290"
     CDS             372..1739
                     /locus_tag="PLG78_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22163.1"
                     /translation="MANPHERQILTSTEKRKIWNVIFASSAGTVIEWYDFYIFGALAT
                     VISGQFFPKDHPTAGFLATLATFATGFVVRPFGAIVFGRIGDLVGRKYTFLLTLLIMG
                     GSTFAIGLLPSFEQVGVLAPILLVSLRLLQGLALGGEYGGAATYVAEHSPDARRGFYT
                     SFIQTTATLGLFVSLGVILGTRLVLGEKDFGTWGWRVPFLLSVLLVITSYYIRLKLRE
                     SPVFVKLKEEGNTSTSPLKDSFGNKANLKLVILALFGATAGQGCVWYTGQFYALTFLQ
                     KTLNIEFVTSNVIVALALVFATPFFVFFGSLSDKIGRKRIMMLGCALAALTYVPIYMA
                     MKEIGNPDNATASHTVSLQAAGLLVILVFIQVIYVTMVYGPIAAFLVELFPTRIRYTS
                     MSLPYHIGNGVFGGLTPFIATAVVASTGNPFSGLVYPIAVATLTLIVGGLYLKDHRHM
                     KISSF"
     gene            complement(1765..2382)
                     /locus_tag="PLG78_03295"
     CDS             complement(1765..2382)
                     /locus_tag="PLG78_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE22164.1"
                     /translation="MLVLLVLIASCSKPFPEADSLRLLVFARAQDWNKYIFITTATHD
                     GNFAAGFATAIAGADDFCAQQKSSSAPWLPGFSAEYRALLTDGATRRASLTADVGDGQ
                     TGWVLAPNTTYYRPDGMVLFTTNAVALYNMNTGLRNPFTSDASAAARWWTGLDLGWET
                     LLTCSSWTSSVANGTRGQGGTTTNASLSAASNGGGTPIRLLCVRQ"
     gene            complement(2399..3148)
                     /locus_tag="PLG78_03300"
     CDS             complement(2399..3148)
                     /locus_tag="PLG78_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNE22165.1"
                     /translation="MTLEWEPVALAKGYVVQIRTSDNDIKEVRVSENRMEQSLNPGEY
                     QVRLAALNKFGKPGAFTDWITVKIRSRDAGVVRLSRDEKSTDPDRGKPAPPQTVQPGG
                     SEEGRRHVSVWPGALIPGYIQIRGDHPYRGLGEITALSAIAIAFYTERRAGDKISSDP
                     FNKPVVLFPLTWSLPASVGSYFYLRRENEKHRYRLHQDNQRGLAALGLLVYAGQCVDA
                     VFFSSPRAKASFQFSPDAQGFRSQVALTFFY"
     gene            complement(3211..4722)
                     /locus_tag="PLG78_03305"
     CDS             complement(3211..4722)
                     /locus_tag="PLG78_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE22166.1"
                     /translation="MRRIKLNEILVPLICGIVLIVVSISLFQDLTGRSKSEGEVIGFI
                     TYRYRVAQRRAASRVVWEDAEQKDSVYNQDTIRTDEKSEALVTLRNGTMIELDPQSMV
                     VLSMRNDAGEIKLAQGSVVLRKPTSEDRLTFEQGGKTEFIKPTGELRLTLNGKSAEAS
                     GSFEFERKGESFKSERDSIRVEESRVERSRPSFSELFPPDNFRVFTDRDRQDITFTWA
                     GAALNLEVSESRSFTSPLLRQSVSGVNSFKTPLPEGIYYWRILAEGKTSLIHKFRIVR
                     TTEVRLLSPIGNIDLRDDSPFIEFAWSESKLAVSYHLRVATDAQWKNVVLDKEMIRNS
                     GVFPLPPGEYYWQVEAVGALPGSNSSTSIQRFSVKDARVVEKPADKQKEGSASQDVTQ
                     KPQALPDKPEGIVPSPGSVVDMSTRDTLAFQWKPVNGAQRYTIILYQAGGVQVYRVDT
                     KQSMHVLTDLSVLDTGSFAYEIRAYTASGESSSGLIPFSVSLGAQLEKPRLKN"
     gene            complement(4719..6992)
                     /locus_tag="PLG78_03310"
     CDS             complement(4719..6992)
                     /locus_tag="PLG78_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22167.1"
                     /translation="MIRNPLEFKNAFALFHRTWFDDAKETQEFLRLQGVRRVFLLATV
                     EKPGSPEYTQLNSLRDVLESHGFAVAFATVESVLARPESHKRFLSVLNSSLHKEATAL
                     LYSPAAQQDSIYILSTLLLGAGLSPARALQRIVGEYPDQVLLRRLEVYAAEKNKRKAG
                     NTSQPEERPRFTIRTKLLLMAAGVVVTSLTIVTMLGTFFFKKTSETMIQEYNLSLARL
                     IGLQVQSDLQNIVYRSDDVLAGRGEADFFKQNASVVLVRSGARTVINPEISEDRTAAR
                     EDLIAASSRNGPDVERAVVRGSEFLILRSPSKSTTIVLFASRLLDAFRTTRQTNLFQL
                     YLVDRQGNFLLSTAEFPTTSGREVPIVLQMISSPVDNGSRRYEFQGKEYLGSFQTLNF
                     SGLGIVSFVEADRVFEAVYRIQKQNFKITLIVLAIAFLGVFYFSSTLTGPIVRLVSAM
                     RRVQKGDYHTDIVPKTADEVGILTSSFLSMTRGLEEREKIKDAFGRFVHEEVANMAMH
                     GELKLGGEQRRVAIFFSDLRNFTGIAEKMQPEQVVDMLNQYFSAMVECVYETGGVVDK
                     YIGDAIMAHWGAFRPGSNDTKNAVEAAIRMRESLVRLNQEFARRGQPVLRCGCGINTG
                     DVIAGQIGSRKRLEYTVIGDAVNFASRIEYLNKAFGTDILISHDSMREVEGMFHAVQL
                     PTVKIRGKDEPQIVFAILGRAGDESAPQTLDELRMRLGIQFDAEAARKELHAPSDQIL
                     SDRPAQGSDSGSREARS"
     gene            complement(7016..8179)
                     /locus_tag="PLG78_03315"
     CDS             complement(7016..8179)
                     /locus_tag="PLG78_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22168.1"
                     /translation="MLRTTALVLIATLAWCSPEKTGEAKPSQPAVTETPRPGLPELAR
                     FLAGKMEPGESRLGKLAALPGYKQHKTQVSGIWSMYVKDFSVPLQEWREQNVPAVKSK
                     TVFYPFAGPDFPNANTIYPNGETYIMIGLEPAGFAPDFESMSDHKAMSELRRMLQSVD
                     YVSRRSYFITSFMNQSYIITEGVAPVILMYLGLLDFVPYSVKAIDLDPNGKIRYLSDE
                     EVKQYRQQRKWTHVEVRFQAAAGQPLKTIYYFKQDVQDEALEKDPRLLKYLESLPQYA
                     SVFKAASYLIHYETFSRIGDVVVNKSELIVMDDTGPRMKKLQGQFDVTVFGHYGRPIP
                     PFQSQYQSDLAKMYRDQKPQPIKFRYGYGGSDGSRGILLVTRKQQQTQATPEK"
     gene            complement(8209..9849)
                     /locus_tag="PLG78_03320"
     CDS             complement(8209..9849)
                     /locus_tag="PLG78_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE22169.1"
                     /translation="MHGLIEDIGLSIIVATIIGVITHRLKQPIIVGYLLAGIIIGPQI
                     GPQLVHDPEHINIISEIGLVLLLFIIGLEMNPQHLMAGGRAILFAGLGQFPLTVMLGV
                     LLFSAAGFAAGFIDAVYLAFACGLSSTAIVVKALFDKFELDSIPGRLTVGILIFQDVW
                     AILVLALQPNLANPTLAPVLGAILRAVFLLGLGFVFSKYVLSRVFHWISRAPEMVVAV
                     SIGWCAFFAALANQIGLSFEMGALIAGVSISSFPYSEHVTEKILPLRDFFLTLFFISL
                     GMKIPYPQPGLLLTVPVLLGFVVLSRFLIVYPLALLGGSGKRTALIASLNLSQISEFS
                     LVIASIGVARSHISSETMSAILYSLAVASVLSSYVLKYNHQLYGFIVRYAPFFSDSRE
                     SAETSSHAKKDICVLGFHKGARAFVDRIATMKPELLKGMIIVDYSVEVLKEVRDRYSV
                     QILFGDISAADTLERAGVREANYIVSTIPDALLRGTTNQKIVRVCRSLSTEAFIAATA
                     DSRDQAEGLRTEGANQVLLPYVVAGERLAMELVGRHRN"
     gene            complement(9904..10659)
                     /locus_tag="PLG78_03325"
     CDS             complement(9904..10659)
                     /locus_tag="PLG78_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22170.1"
                     /translation="MTRIARLTIAALLLAEAATPALALGTYAEGQVYLTVRKFESSGI
                     IFESWEGTGELVTVDRSENCKQEENECYTPKKEMISFSVRPETDGGKLAAEIQKNINK
                     EILVAYRIHRIEAAALSTDFEVTGLLEMQDRLAADLPAKKVTRKTGAKRNFSVLGRVV
                     HLEYAGTAVGTYEGIYIDQQKGKAHPFSITDSDMAAHIYKVMALRKDYNLGISQAFVT
                     GVRKSDYDIFEVNMNEPAGAADPQPPETKPDAK"
     gene            complement(10709..11296)
                     /locus_tag="PLG78_03330"
     CDS             complement(10709..11296)
                     /locus_tag="PLG78_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE22171.1"
                     /translation="MARQFSAQASSELETIIADFPNKKSALLMVLRLAEREFGFLDDS
                     AMELVARSCDVSPAHVLGMVTFYTHFKRPYHGKHRFMVCATLMCSIGGPHGKTDDALQ
                     VLKEKLGLAPGERSEDGLFSVEKVECLADCNQPPVMQKDNEHKVRMGKEELSRYVDDL
                     LKKEGLSAGHYSGKPVVRMDPNVPHISVKQSVPAH"
     gene            complement(11369..>11920)
                     /locus_tag="PLG78_03335"
     CDS             complement(11369..>11920)
                     /locus_tag="PLG78_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE22172.1"
                     /translation="WYMIGSFVLLTVWHETWFYWAHRAMHSKLLFKHVHLVHHRSINP
                     TPFAAYSFHPIEAVLEGLYLLIFTMVIPSYRYMVLFHTFYAMLLNIYFHTGYEFFPKN
                     FLRGRITKWINTSTHHNMHHSKTHGNYSLYFNFWDRILGTNHPEYQKTFEEIAGRAAD
                     ASGGSPSHRPRSGHGVGKEAEAT"
BASE COUNT         3043 a         3076 c         2892 g         2909 t
ORIGIN      
        1 gtaaaacaca atcaaagtcc acagtggact atggaaagac aggatgccat cgagctcttc
       61 ggaattctgc tcgacaatgc atgcagattc tgcaccggcc aggtggtcat cggactcgac
      121 acatcagaag ttacgatcga agacgatggc cccggatgca cagaggagca aatgaaacaa
      181 ctgtctttac gcggagctag aattgacgaa ttcacagagg gcgggcttgg tctttccatc
      241 gcgcgagagc tggcagaaag ctaccagatg actctttcat tctcgcgttc gccttcagga
      301 ggactcagag tttctatccg caactcataa aatcttcttg atgtaaggcg accgccagac
      361 ctcctgctcg catggcaaat ccgcatgagc gacagattct caccagtacg gagaaaagaa
      421 aaatctggaa cgtgatcttc gcatcctctg cgggaacggt cattgaatgg tatgatttct
      481 atatattcgg ggccctggcc acagtcatct ccggccaatt ctttccaaaa gatcatccga
      541 cggctggatt tctggcaacc cttgctactt ttgcaaccgg ttttgtagtc agaccgtttg
      601 gtgccatcgt cttcggacgc ataggcgatc ttgtcggaag aaaatacacg ttcctgctga
      661 cactgctgat catgggcggc tctactttcg ccattggtct tttgccgtct ttcgagcagg
      721 ttggcgtact tgcccccata ctcctggttt ccttgcgcct tcttcagggc ctggcgctgg
      781 gaggcgaata cggcggcgcg gccacttacg tagcagaaca ctcaccggac gcaaggagag
      841 gcttctacac aagtttcatc cagactacgg caacgctggg tctatttgtt tccctgggcg
      901 tgatccttgg aaccagactc gtcctcggcg aaaaagattt tggcacgtgg ggctggcgcg
      961 ttccattcct actttcggtt ctacttgtaa ttacttcgta ctacataaga ctcaaactca
     1021 gagaatcgcc tgtatttgtt aaattgaagg aagaaggaaa cacatccacg agccctctga
     1081 aagattcttt tggaaacaaa gcgaatttga aactggtcat ccttgcattg ttcggagcaa
     1141 ccgcgggcca gggctgcgtg tggtatacag ggcagtttta cgcactcact ttcttgcaga
     1201 aaacactgaa catagaattc gtaacgtcga acgtgatcgt cgctctggcc ctggtgtttg
     1261 cgacgccgtt ctttgtattc ttcggatctt tgtcggacaa aatcggtaga aagagaatca
     1321 tgatgctggg ttgcgctctg gccgcgctca catacgttcc catttacatg gcaatgaaag
     1381 aaatcggcaa ccctgacaat gccactgcgt cccatacagt ttcgctccag gcagccgggt
     1441 tactcgtgat tcttgtattt attcaagtaa tctatgtgac gatggtgtac gggcctatag
     1501 ccgcgtttct agtggaattg tttccaacga ggatccgtta tacgtccatg tctctgccct
     1561 atcacattgg aaatggagtt ttcgggggac tcactccatt cattgcgacg gccgtagttg
     1621 catccacagg caaccccttc tctggtcttg tgtatcctat cgccgtggct acactgacgc
     1681 tcattgttgg tggtctctat ctaaaagatc acagacacat gaagatatcc agcttctagt
     1741 tttgcttgct tcttgaattg cagcttactg tcgcacgcag agaagacgaa tcggcgtgcc
     1801 accaccattg ctcgctgcag aaagagaagc gttcgtggta gtgccaccct ggccccgggt
     1861 gccgttcgct acagaggaag tccaggaaga gcaagtaagc agggtttccc agcccaggtc
     1921 aagtccagtc caccatcggg ccgccgcact tgcatccgaa gtaaatggat ttcgaaggcc
     1981 agtgttcatg ttgtaaagcg caaccgcatt ggtagtaaat aagaccatac catctggtct
     2041 gtagtacgta gtattggggg caagtaccca gccggtttgt ccatcaccca catccgcagt
     2101 cagacttgcg cgccgtgtcg cgccgtccgt caggagcgct cgatactccg cggagaatcc
     2161 tggaagccat ggggcgctcg aagatttctg ttgcgcacaa aaatcatctg ccccggcaat
     2221 agcagtcgca aacccggccg cgaaatttcc gtcgtgcgtg gctgttgtga taaagatgta
     2281 cttattccaa tcctgagcac gcgcaaaaac cagaaggcgt agcgagtccg cctctggaaa
     2341 tggtttcgaa caggaggcaa tcaaaaccag gagaacaagc atgcgcgtcc gcatacttct
     2401 agtagaagaa agtcagggct acttgcgagc gaaatccttg cgcatctggc gaaaattgaa
     2461 agctcgcttt cgcgcgcggc gaagagaaaa agactgcgtc cacacattga cccgcgtaaa
     2521 ccagaagacc gagcgctgcc agtcctctct gattgtcctg atgcaatctg taccgatgct
     2581 tctcattctc tcgtcgcaag taaaagtaac tgccgacgga agcagggagg ctccacgtga
     2641 ggggaaaaag aactaccggc ttattgaaag gatcgcttga gattttatct cctgctcttc
     2701 tctctgtgta gaaagcgatc gcaatcgcag agagagcggt aatctctcca agaccgcggt
     2761 atgggtgatc ccccctgatt tgaatgtagc ccgggataag agctcctggc cagacggaca
     2821 catggcgccg tccctcttcg cttcctccgg gttggacagt ttgtggtggc gcaggctttc
     2881 ctctgtctgg atccgtcgat ttctcatcgc gcgaaagcct cacaacaccg gcatcccgcg
     2941 agcgaatttt gaccgtgatc cagtccgtga atgcaccggg cttcccaaac ttattgagcg
     3001 cagccagacg cacctgatat tctcccggat tcagactttg ttccattctg ttctctgaga
     3061 ctcgcacttc cttgatgtcg ttatcacttg tgcgaatttg aaccacgtat ccctttgcca
     3121 gtgctacagg ctcccattcc agagtcattt tctgttcagc acgcagcggt agagaaaaga
     3181 tcagaacaac gaaaacggaa aggagcgatt tcaatttttc aaccttggct tttcaagctg
     3241 agcacccagt gacacagaaa acggaatcag tccagatgaa ctttctccag acgcggtgta
     3301 tgcgcgaatc tcataggcaa aggagccagt gtcgagcact gaaagatcag tcagaacatg
     3361 catggattgc tttgtatcca cacggtagac ctgtacgccg ccagcctggt agagaatgat
     3421 cgtataacgt tgtgccccat tcacaggttt ccattgaaac gcaagtgtgt ccctggtaga
     3481 catgtccacg accgatccag gagaaggaac gatcccttct ggcttatcgg gcaatgcttg
     3541 aggtttttga gttacatcct gcgaagcaga tccttctttc tgtttgtccg ctggtttttc
     3601 aaccaccctt gcgtctttga ctgaaaatcg ctggatggaa gtactgctgt tcgaacctgg
     3661 aagcgcacca actgcctcta cctgccagta atattctccc ggaggaagag gaaaaactcc
     3721 agaattgcga atcatttctt tatccagcac gacattcttc cactgagcgt ccgtagccac
     3781 acgcaaatga tacgaaacag caagcttgct ttccgaccac gcaaactcaa taaaaggact
     3841 gtcatcgcgt aaatcaatgt ttcctattgg ggaaagtagc cgaacttctg tagtgcgaac
     3901 aattcgaaat ttgtgaatca ggctggtctt tccttcagca agaattctcc agtagtagat
     3961 cccttcgggc aatggagttt tgaacgaatt cacgccagaa acgctctgtc tcagaagtgg
     4021 agaagtaaat gagcgtgatt cacttacttc cagattcaag gccgctccag cccacgtgaa
     4081 agttatgtcc tggcggtccc gatcagtgaa aactctaaag ttgtccggtg gaaaaagctc
     4141 cgaaaaagag ggcctgcttc tctcaactcg gctttcttct acgcgaatcg aatcccgctc
     4201 acttttgaaa gattctcctt ttctttcaaa ctcaaagcta ccggatgcct ctgccgattt
     4261 cccattcaac gttagtcgca attctccggt tggttttatg aattccgtct ttccaccctg
     4321 ctcaaacgtc aggcgatctt cactcgtagg ttttctgaga acaacagatc cttgcgcaag
     4381 cttgatttcg cccgcatcat tgcgcatgga cagaaccacc atactctgtg gatccagctc
     4441 tatcatcgtg ccattgcgca gcgttacaag agcttccgat ttttcgtcgg tgcgaattgt
     4501 atcctggtta tacactgaat ctttctgctc tgcgtcttcc cagactacgc gagatgcggc
     4561 tctgcgctgt gccacgcggt atctgtatgt gataaagcca atgacctcac cttcggattt
     4621 gcttctaccc gtaagatcct gaaaaagcga aatcgaaact acaattaaga cgattccaca
     4681 gatcaagggt acaagaatct cattgagctt gatacgtctc aactgcgggc ctcgcgcgat
     4741 ccagaatcag acccttgagc cgggcgatcc gagaggattt gatccgatgg tgcatgcaat
     4801 tccttgcgcg ctgcttccgc gtcaaattga atgcccagtc gcatccgcag ctcgtcgaga
     4861 gtctgggggg cggattcatc ccctgcgcgc ccaagaattg caaaaacaat ttggggctca
     4921 tctttgccgc gtatctttac ggtaggcaat tgcacggcat gaaacattcc ttccacttcg
     4981 cgcatagaat cgtgcgaaat caaaatgtct gtaccaaagg ctttattcaa gtattcaatc
     5041 cgagatgcaa aattcaccgc atccccgatc acagtatact caagacgctt tctcgatccg
     5101 atctgtcctg caatcacatc cccggtattg atcccgcatc cgcatcgcaa gactggctgg
     5161 cctcttcgcg caaactcctg attcaatcga accaggcttt cgcgcatgcg aatggctgcc
     5221 tccactgcgt tttttgtgtc attgctcccc ggtctgaagg ccccccagtg cgccatgatc
     5281 gcatcgccta tgtatttatc cacgactccg cctgtttcat agacacattc aaccatggcg
     5341 gaaaaatact gattgagcat atccacgacc tgctccggct gcatcttctc tgcgatcccc
     5401 gtaaaattcc gcaaatcaga aaagaaaatg gcaacgcgac gctgttctcc gcctaacttc
     5461 agttcgccgt gcatggccat gttggccact tcctcatgaa caaagcgacc aaaggcgtcc
     5521 ttgatctttt ccctttcttc gaggccgcgc gtcatagaca agaatgagga agtaagtatg
     5581 ccaacttcat ctgccgtttt cgggacaatg tctgtatgat agtctccttt ctgaacgcgg
     5641 cgcatggcgg atacaaggcg cacaatgggt ccagtgagag tagatgagaa ataaaagacg
     5701 cccagaaatg cgatcgcaag tacaatcaga gtaattttga aattctgttt ttgaatcctg
     5761 taaacagcct cgaaaaccct gtctgcctcc acaaacgaaa caatgccaag tccgctgaaa
     5821 ttcagtgttt ggaatgaccc gaggtattcc tttccctgaa actcatatct cctggacccg
     5881 ttgtccactg gacttgagat catctgcaga acgataggta cttcacgacc acttgtagtc
     5941 ggaaactccg cggtagagag aagaaagttc ccctgcctgt ccaccagata cagttgaaag
     6001 agattcgttt gacgcgtcgt gcgaaatgca tcgagcaatc gcgacgcaaa gagaacaatc
     6061 gtggttgatt ttgatggaga acgcagaata agaaattcac tgccacgaac caccgctctc
     6121 tctacgtccg gtccgtttct agaagaagca gcgatcaaat cctctctggc cgcagtgcga
     6181 tcttctgaaa tttccgggtt gatcactgtg cgcgcaccgg agcgcacgag aacaacagaa
     6241 gcattctgtt tgaaaaaatc tgcttctccg cgtcctgcaa gaacgtcatc ggaacgatag
     6301 acgatattct gaagatctga ttgaacctga agtccgatca gacgagccag actgagattg
     6361 tattcctgga tcatggtctc gcttgtcttc ttgaagaaga aagttccaag catcgtaacg
     6421 atggtgagag aagtaaccac aacacccgcg gccatcagaa gaagcttggt tcgaatggtg
     6481 aagcgcggcc tctcttcagg ttgagaagta ttcccagctt tacgtttgtt tttctctgcg
     6541 gcatacactt cgaggcgacg aagcaaaacc tgatcgggat attcgcccac gattctctgg
     6601 agagctctag caggagaaag tcccgcaccc agaagcaaag tagaaagaat gtagatcgaa
     6661 tcctgttggg ccgccggcga atacaaaagt gctgtggctt ctttgtgaag ggatgaattg
     6721 aggacagaca ggaaacgctt gtggctttca ggacgggcga gaacggactc tacggttgcg
     6781 aaggctactg caaatccgtg cgactccagc acgtcacgaa gcgaatttag ctgagtgtat
     6841 tccgggcttc ctggcttttc taccgtagct agaaggaaga ctcgccgaac accctgtaga
     6901 cgaaggaatt cctgagtctc tttcgcatcg tcgaaccatg tgcgatggaa gagcgcaaag
     6961 gcgttcttga actcaagcgg attccggatc atagaaactc cccggaatca gacgcctatt
     7021 tctccggcgt cgcctgtgtc tgctgttgct ttcgagttac aagaagaatg cccctgctac
     7081 catctgaccc gccatagcca tagcgaaact tgattggctg cggtttttga tctctgtaca
     7141 tctttgccag atctgattgg tactgagatt gaaatggagg aatgggccta ccgtagtgac
     7201 cgaatacagt cacatcaaat tgaccctgca atttcttcat acgtggacca gtatcgtcca
     7261 tcacaatcaa ctctgacttg tttacaacta cgtcaccgat tcgactgaat gtctcatagt
     7321 ggatcaagta ggaagcggct ttgaaaacag acgcatactg aggaagagat tcgagatact
     7381 tcagaaggcg tggatctttt tccagcgctt catcctgaac gtcctgcttg aagtagtaaa
     7441 tggtcttgag aggctgaccg gccgccgcct ggaagcgaac ttctacgtgc gtccactttc
     7501 tctgctgtcg atattgtttt acttcttcat cgctcaaata gcgaatcttt ccatttggat
     7561 ccagatcgat tgcttttaca gaatacggaa caaaatccag aagccccagg tacatcaaaa
     7621 tcacaggtgc cacaccttct gtgatgatgt aactctggtt catgaatgaa gtgatgaaat
     7681 aagatctgcg cgaaacgtag tctacgctct gcagcattct gcgcaattcg ctcattgctt
     7741 tgtgatcaga catgctctca aaatcaggcg cgaaaccagc cggctcaagg ccgatcatga
     7801 tgtacgtttc accgtttgga tagattgtat ttgcgtttgg aaaatcaggg ccggcgaaag
     7861 gataaaaaac tgtctttgat tttactgccg ggacgttctg ctcgcgccat tcctgcagag
     7921 gaacggaaaa gtctttcaca tacatggacc agataccgga gacttgagtc ttgtgctgct
     7981 tgtatccagg gagcgcggca agcttcccca gtctactttc gcctggctcc atcttgccgg
     8041 caagaaaccg cgcgagctct ggcagaccgg gccgaggtgt ttctgtgact gctggctgcg
     8101 aaggctttgc ctctccggtt ttttcaggag aacaccaggc aagagtggcg atcaatacaa
     8161 gtgcagtagt gcgaagcatg gcccaggata ggaaatggac tgccggagtc aatttctatg
     8221 acgaccgacc agttccatgg ccagtcgttc gccagctaca acgtatggca agagcacctg
     8281 gttagcccct tccgttcgca gaccttctgc ctggtctctg gaatcagcag tggccgcgat
     8341 gaaagcctcg gtagacaggg atctacatac gcggacaatt ttctggttcg tagtgccccg
     8401 caaaagcgcg tccgggatcg tagaaacgat gtagtttgcc tcccgaactc ctgcccgctc
     8461 gagagtatcc gccgccgaaa tatcgccgaa caggatttga accgaataac ggtccctgac
     8521 ttccttaagt acttccacac tgtagtctac aatgatcatt cctttgagta actctggttt
     8581 cattgtagcg atccggtcca cgaaggcccg cgctcctttg tgaaagccaa gcacgcagat
     8641 atcctttttt gcatgcgaag aggtttccgc tgattccctg gagtcgctga agaatggcgc
     8701 ataacgtaca atgaacccgt acaactgatg attgtatttc agaacatacg aggaaaggac
     8761 agaggcaact gccagactat agagaatcgc agacatggtt tccgaagaaa tgtgcgacct
     8821 ggccactcca atggaggcaa tcacgagaga gaattcactg atctggctta gattcagaga
     8881 agcaatcaat gcagtcctct tcccggatcc accgagtaga gcgagaggat atacaattaa
     8941 gaagcgcgag agaacaacga agcccagcaa taccggaact gtgagtaaaa gaccgggctg
     9001 cggatagggg atcttcatac caagagaaat aaaaaacaga gtcagaaaga aatcgcgaag
     9061 aggaagaatc ttctccgtca catgttcact gtaaggaaaa ctggagatag agacgcctgc
     9121 gatgagagca cccatttcaa acgagagtcc aatttgattt gccagtgccg caaagaaagc
     9181 acaccaacca atagacacag caacgaccat ttcaggtgcg cgcgaaatcc agtgaaacac
     9241 tcttgaaagc acgtacttac tgaacacgaa accaagtccg agaaggaaga ctgcgcgcaa
     9301 gatcgctccc agaaccggtg caagggttgg atttgcaagg ttaggttgaa gagcaagcac
     9361 gaggatggcc cacacatcct ggaagatcaa aattcctacc gtcaggcgac ctggaatcga
     9421 atccaattcg aatttatcaa agagtgcttt aacaacaatc gcagtactgg aaagcccgca
     9481 ggcaaaagcc agataaacgg catcaatgaa acccgcagca aagccggcag cactgaataa
     9541 caatacacca agcattactg tcagtggaaa ctgaccaagc ccggcaaaca ggatcgctcg
     9601 tcctcctgcc atcagatgct gtggattcat ttccaggcca atgatgaata gcagaagaac
     9661 cagaccaatt tcagagatga tgttgatgtg ctctggatcg tgaacgagct gcggcccaat
     9721 ctgcggacca atgatgattc cagcgagaag atagcctacg atgatcggct gtttcagtct
     9781 gtgcgtaatt acaccgatga tcgtcgcaac gatgatcgaa aggccgatgt cttcaatgag
     9841 tccatgcatg ggaatagaaa cgcgggctgt tgcccgcgtc gaaaggaact agaaacgtac
     9901 gatctactta gcgtccggct ttgtttcagg aggctgcgga tctgccgctc ccgcaggttc
     9961 attcatgttg acttcgaaaa tatcgtagtc tgacttgcga actcctgtta cgaacgcctg
    10021 gctgattcca agattgtaat cttttctcag agccatcact ttgtaaatgt gagctgccat
    10081 atcagaatct gtgatagaga aaggatgggc ctttcccttt tgctgatcaa tgtagatgcc
    10141 ttcgtaggtt cccactgcag ttcccgcata ttccagatga accacgcgac cgagcacact
    10201 gaagtttcgc ttagcgcctg ttttgcgagt cactttcttc gcgggaagat ccgccgccag
    10261 acgatcctgc atctcgagga gtcctgtgac ttcaaaatct gtagaaagag cggccgcttc
    10321 tattctatga atgcgatagg cgaccaggat ttctttattg atattctttt gtatttccgc
    10381 tgccagcttt ccgccgtctg tttcaggacg aacgctgaac gaaatcattt cctttttggg
    10441 agtatagcat tcattctctt cctgcttgca gttctcagag cgatccacag taacgagttc
    10501 gccggtccct tcccaggatt cgaaaataat gccgctgctt tcgaacttgc gaacagtcag
    10561 atacacctga ccttctgcat aggttccgag agccagggca ggagtagcag cctctgccag
    10621 gagaagcgct gcaattgtta gtctagcgat gcgagtcatg ggaagggtat gaagcaccct
    10681 tctgatgtgt cacgtaactt ttgagcgact aatgggcggg aacactctgt ttcacggata
    10741 tatgaggcac gttcggatcc atgcgaacca ctggcttgcc ggaatagtgc cccgcgctca
    10801 gtccctcttt ctttaagaga tcatccacat atcgcgacag ttcttctttg cccatgcgaa
    10861 ctttgtgttc attgtctttt tgcatgacag gaggctggtt gcaatccgca agacactcca
    10921 ccttctccac ggaaaacaag ccgtcttccg aacgctcgcc cggggcaaga ccaagttttt
    10981 ctttgaggac ttgaagggcg tcatccgtct ttccgtgcgg gccaccaatc gaacacatca
    11041 aagtggcaca gaccataaag cgatgtttgc cgtggtaagg acgcttgaag tgagtgtaga
    11101 atgtaaccat gcccagaacg tgagcagggc tgacatcgca ggaacgggcc accagttcca
    11161 tggccgaatc gtccaggaag ccgaactctc tttctgcaag cctcagcacc atcagaagag
    11221 cggatttctt gttagggaaa tccgcgatga tggtctcaag ctcgctcgat gcctgtgccg
    11281 aaaactgacg cgccatggca atcagagaat cgatggctca tttctttgtc aaacagttcc
    11341 gatccaatcg ccatggaacg cgctttgctc aggtggcctc tgcttccttg cccacaccat
    11401 gccctgagcg cggtctgtgc gacggagaac ctccagacgc atccgctgct cttcctgcaa
    11461 tttcttcaaa agtcttctga tattctggat gattcgtgcc caggatgcga tcccagaaat
    11521 tgaagtagag actgtaatta ccgtgcgtct tcgagtgatg catgttgtga tgcgtagacg
    11581 tattgatcca tttcgtaatc cttccgcgca gaaagttctt tggaaaaaac tcgtatcctg
    11641 tatggaagta aatgttgagc aacatcgcgt agaacgtgtg aaacaaaacc atataacgat
    11701 aggacggaat caccatggtg aagatcaaaa gatacaatcc ttccaacact gcttcgatcg
    11761 gatgaaacga ataggcggcg aaaggagtcg ggttgatcga tctgtggtga accagatgaa
    11821 catgcttgaa caagagttta gagtgcatcg cgcgatgtgc ccagtaaaac caggtttcat
    11881 gccagacggt cagcaaaaca aaagagccga tcatatacca
//
LOCUS       DAOQJL010000045        11824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2716_length_11824_cov_4.393916, whole genome shotgun sequence.
ACCESSION   DAOQJL010000045 DAOQJL010000000
VERSION     DAOQJL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_11824_cov_4.393916"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLG78_03340"
     CDS             complement(<1..740)
                     /locus_tag="PLG78_03340"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNE22173.1"
                     /translation="MKEYILAVDIGNTNTVFGLFENTADVEIQKDWRTVTRRDRTSDE
                     LGIFLLGFLQSSGIQSSQIKGFVYSSVVPSFNPIVERMAADYFSSVPLRVRYDNIPLK
                     IDYPRPEEIGADRLVNAVAADRMFPGNQIVVDLGTATTFCVVHEGTYLGGSIAPGLKL
                     SMEALFRNTAQLFPVEFSRPACGVISDSTRTAIQAGFFFGWVGLLRGILEEIHKTYPG
                     KKYHAIATGGLSAMIHREVPEIFAAVDPL"
     gene            complement(737..1360)
                     /locus_tag="PLG78_03345"
     CDS             complement(737..1360)
                     /locus_tag="PLG78_03345"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNE22174.1"
                     /translation="MIALEPNAEKWIRLESVDSTNSFLLREDLPSGTIVIADRQTQGR
                     GRHGRQWVSPEGSLILSGILCFDSEQISDDRLTLLPLLAGVALLRALQKMNPSVNLGI
                     KEPNDIIIDRNARKGKVAGILVESEIKGRERRVIVGVGVNATSAPETSDSVYQPAALF
                     DVSQGEGPFDRDECARLFVMELNARASQIESATPAFLGEVAQYRVGS"
     gene            complement(1357..2475)
                     /locus_tag="PLG78_03350"
     CDS             complement(1357..2475)
                     /locus_tag="PLG78_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE22175.1"
                     /translation="MFKSVRLTRLGLINLILFTLFIGAFLSPLIYSGFRSMVLDHQSS
                     ADPIPESDQKAAIALQQSYMNVFKKASPSVVFIRTRVLVPPRYWFDLYSQQEGAGTGI
                     VIDKAGYIVTNNHVVAGAQKIEVVFPDQRKERAKLIGRDETSDIAVIQIPGSDKLVPA
                     VLGDSDKVAPGQLAFALGSPFGLESTFTVGVISAKHRSIDNTKYTRIQTDTSINPGNS
                     GGPLLNVYGEVVGINQSIISPGGQGGSVGIGFAIPINEAKSVIEQLRKERRVIGRPTL
                     GVQVGVPTPNLRQFLKVSEEYGLVVRFVVPGSAAEDAGMKANDYLLKANDKELREPDE
                     LLEEVAKAGIGGKIKLEFVRGGKKQSVTIAVGEDQSNQ"
     gene            complement(2505..3677)
                     /locus_tag="PLG78_03355"
     CDS             complement(2505..3677)
                     /locus_tag="PLG78_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNE22176.1"
                     /translation="MGYQFIDKQQDLDLAVSVMGRSQYVSVDTESSSFYTYESELCLI
                     QISASGKNYLIDPLANLTVDGLAEIFANPSIVKVMHTAASDISELRRLRKFKFENLFD
                     IMVGCRMLGFVSCSLVSLVKHYTGRDLEKTEQKSNWKKRPLTASQMEYAHRDTIYLEE
                     IMKAMKVEIEKFGFADEFAEDMIRIIKESYPVERIYDHDSWKHVPGAFQLSPIGRGFL
                     KALLEIRESRARKDNIAPFRLGSNDALLNVAERMPDSISELSTFGLHPDFVRKEGERI
                     LEMRNKSPQITNADSPREKRKPPDPALARLKKWRKDVAEYRGFEPSIILSNRQLEAIM
                     EARPSTMEDLARLDLLTDWKLEHYGPMVLSVLAGKEPPLGDLKRLPPERRRPQISG"
     gene            3752..4297
                     /gene="lepB"
                     /locus_tag="PLG78_03360"
     CDS             3752..4297
                     /gene="lepB"
                     /locus_tag="PLG78_03360"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE22177.1"
                     /translation="MALRNRDLVPEKRAPVKKSVIVAGLVAAAILALMLRLVVHAVWI
                     FPLRVGTASMEPAVKKGQTVYVVKRVELQFGDIILYKHPDNPDVRLLGRITGLPGDAI
                     EMKEKQLFRNGAPVQDAWQIRTQTGYSLPGSVFPRDSFAERKITTGKYFVLCDLRTEC
                     LDSRLLGEISREMIVGRIRPD"
     gene            complement(4635..5963)
                     /locus_tag="PLG78_03365"
                     /pseudo
     CDS             complement(4635..5963)
                     /locus_tag="PLG78_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395243.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            6056..8263
                     /locus_tag="PLG78_03370"
     CDS             6056..8263
                     /locus_tag="PLG78_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE22178.1"
                     /translation="MQLPDEKRLELRTSRLLLVIGLVSYAIFWVIIDRLGMRDPQPLR
                     YIAVMILIAAALAASFVSNFAKKHLRKLVIILLYVIASHFFLLLYTFRLAPVQMIDML
                     IFLSAVTATSTFIFESRAGLLSFLLFSTAGAAVAAYAVKDPLVDIYQFFAAIAVLGFV
                     GFMAMAYQMRMLDKIRGSEQQLRNAIQQSHRYDFIVNTSREFMTLINREYKYEAVNDA
                     YCEAHHKPREILIGRPVTEVWGGDLFERVIRGYLDECFKGNIVNYGVGFEFPALGPRF
                     FDVTYYPYRGAGDEITHAVVVSRDHTEKKIAEDRLIHEALHDALTGLPNRHLLTDRIG
                     HALKKKTREDDFDFALLFLDLDRFKIVNDTMGHLAGDKLIIAVARVLEKCTRPGDTVG
                     RLGGDEFVLLLENTNLQGAEAVASRILRELSQPLMLGDSEVFTGASIGIAPYVSGYTR
                     SEEMLRDADTAMYRSKALGRSRYTIFDEEMRFAIKEQLTLEAEMRHGLERGEFTVYFQ
                     PIFKLTDGTLTGYETLLRWKHSRRGVLGPSVFLAVAEEANLIDPLGEFVLREACRFLS
                     RLPGKDTFVSVNVSAKQFQRHGILESVIQSLADFSAEPSRLMLELKEASFARGFDEVA
                     SILRALSRLGVKLAVDNFGTGNASIVNLKGLPLHMLKIDGSFARGLPHDASDRALVAG
                     MISIAHSLGIVAGATMVETASQETLLRSLGCDTAQGFHFAEPADSSAWLSQAR"
     gene            complement(8260..9075)
                     /locus_tag="PLG78_03375"
                     /pseudo
     CDS             complement(8260..9075)
                     /locus_tag="PLG78_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9307..10041
                     /locus_tag="PLG78_03380"
     CDS             9307..10041
                     /locus_tag="PLG78_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22179.1"
                     /translation="MRRIFQIIAAVTLACPLHAESSENEMQSYFLSGAAAASGILEGG
                     VIAPAWHAYIRDVPRIDRVQSLILFSSLDRPGAALTTEIFRQESQTDYRHAANLRLQA
                     YGGTLALFGLAGVLATKPVPSWDHPAVPVLGGVSAGMSLRWLVQWNRYSNVMRQLSTN
                     QRTTQIALLAAAPSDRLTLVSYYLRQTEKKTFARTRRLESACASVDFALGAAAFFYAY
                     ASTNPAPSTSAFFFAGPDRAGFQIRF"
     gene            10168..10797
                     /locus_tag="PLG78_03385"
     CDS             10168..10797
                     /locus_tag="PLG78_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22180.1"
                     /translation="MNLPLTSISPPRLRRISLLTGVSGIYALSHSWISRLALLGLPIT
                     VMDCADRFDPFAIAEEAASQDVKPDVLLETITCQKIATPLQFLGAARGILNARRGGVA
                     VILSPLRHFLDIGESAHGRELLLDGFLSVLSELSVRSRPVLLVEAQQDAPLFHSAFDR
                     MAALASPIWRILPDSVEVAENKKTQTTRSVDRMAQNNLEGISPDRLATG"
     gene            complement(10794..>11824)
                     /locus_tag="PLG78_03390"
     CDS             complement(10794..>11824)
                     /locus_tag="PLG78_03390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE22181.1"
                     /translation="VVFLGGSDEMQMRLRSVRFGGSGFFSKPVNVDALVTLLDRLTSP
                     FVVQAYRILIVESDGELSQHLVRILGAAGMETHVVSNPAEMLRAGDLFHPELILMNLY
                     YPEILGMELATVLRQDPRYERIPIVFLSSELDPARQMAALAKGGDDCLTLPIHPDYLA
                     LIVASRVEATRSIKSLYFRDGLTGLLNHRAMLDSLEMKLELAGARGVELPFVMVDIDR
                     FKLINDTYGHPVGDTVLKSLGRLLKQRVWSKDLVGRLGGEEFGVIVNSMDAATAARVF
                     NEIRIDFAQIRHTSSEGDFYATFSCGVAWFPRYATSAEIESAAERAVRQVKALGGNRV
                     GLIGRHES"
BASE COUNT         2933 a         3104 c         2823 g         2964 t
ORIGIN      
        1 agcggatcca ccgctgcaaa aatctcgggg acttctctgt gaatcattgc agaaagcccg
       61 cccgtggcga tcgcatgata cttcttaccc ggatatgtct tgtgaatctc ttccagaatg
      121 ccgcgaagaa gtcccaccca gccaaaaaag aatcctgctt gaatggctgt gcgcgttgag
      181 tcagaaatca caccgcatgc aggtcttgaa aactcaactg gaaacaactg cgcagtgttg
      241 cgaaacaaag cttccataga aagcttgagc cccggcgcaa tgcttccacc aagatacgtt
      301 ccctcgtgaa ccacacagaa ggtggtcgcc gtacccagat ctacaacgat ctgatttcca
      361 gggaacatcc tgtctgcagc cactgcgttt accagtctgt ctgctccaat ttcctctggc
      421 ctgggataat caatcttgag cggaatgttg tcgtatcgca cgcgaagtgg aacggaactg
      481 aaatagtcag cggccattct ctctacgatt ggattgaaag acggaaccac agacgaatac
      541 acaaagcctt tgatctgcga agattgaatt cctgacgatt gaagaaaacc caatagaaaa
      601 atacctaatt catccgaagt gcgatcccga cgcgtcactg tcctccagtc tttctggatt
      661 tcaacgtctg ctgtattttc gaacaaacca aaaactgtat tcgtatttcc aatatctaca
      721 gccagaatgt attctttcat gaacctaccc tgtattgtgc cacttctccc agaaatgcag
      781 gtgtggcact ttctatctgc gatgcccttg cattgagttc catgacaaaa agtcgagcgc
      841 actcgtcacg gtcgaatgga ccttcccctt gcgagacatc aaagagcgct gccggctgat
      901 acaccgaatc agatgtttcc ggtgcactcg ttgcattgac tccaacgcca acgatcactc
      961 gcctttcgcg gcctttaatt tctgattcta ccagaatgcc cgctaccttg ccttttcgtg
     1021 catttctatc aatgatgatg tcgtttggtt ccttgatccc taaattcaca gacggattca
     1081 ttttctgcaa agcccgaagc agagccactc cggcaagcaa tggaagcaac gtcaggcgat
     1141 catctgaaat ctgttctgaa tcaaagcaga gaatgccgga taaaatcaaa gagccctcgg
     1201 gagaaaccca ctgccttcca tgtctacctc tgccctgagt ctgccgatct gcgatgacga
     1261 ttgttccgga aggaagatct tcgcgcagta ggaaactgtt ggttgaatca acgctttcga
     1321 gccgaatcca tttttctgca ttcggctcaa gagcgatcat tgattcgact gatcctcgcc
     1381 aacggcaatc gtgacggact gcttcttgcc accacgaacg aattcgagtt tgatctttcc
     1441 gccaattcct gctttggcca cttcttctag aagctcgtcc ggctcgcgaa gctctttgtc
     1501 atttgctttc agtagatagt cattggcttt cattcccgca tcctcagccg cagaacctgg
     1561 aacaacaaat cgaactacga gtccatattc ctcgcttact ttcaaaaact gtctcaggtt
     1621 cggtgtagga actcctacct gcactcccag agtaggtctt ccaatgacgc gacgctcttt
     1681 gcgcaactgt tcgatcacgc ttttggcttc attgattgga atagcaaaac caattccaac
     1741 gcttccgccc tgaccgcccg gagaaatgat agactgattg attccaacta cttccccgta
     1801 aacgttaaga agtggtccgc ccgagtttcc cggattgatg gaagtgtctg tctggatgcg
     1861 agtgtatttg gtattgtcta tgcttctgtg cttcgcagaa atcacaccca cagtgaatgt
     1921 actttccagg ccaaacggag atccaagtgc gaatgcaagt tgaccggggg ccactttgtc
     1981 tgaatcgcca agaactgcag gaaccagttt gtcgctccct ggaatttgaa tgactgcgat
     2041 gtcgcttgtt tcatctcttc ctatgagctt tgctctctct tttctttgat ccggaaaaac
     2101 aacttcaatc ttttgtgcgc cagccaccac gtgattgttg gtaacaatgt aacccgcttt
     2161 gtctatgacg atgccggtgc ccgcaccttc ttgctggctg tagagatcaa accagtaccg
     2221 gggcgggact agaactcgag tgcgaatgaa tacaacggat ggagatgctt tcttaaaaac
     2281 gttcatgtaa ctctgctgca aggcgatggc cgccttctga tcgctctctg gaattggatc
     2341 tgcgctcgac tggtgatcca ggaccatgct gcgaaagccg ctgtaaatca aaggactcaa
     2401 gaatgccccg atgaaaagag taaacaggat gaggttgatc agcccgagac gagtcagacg
     2461 aacagatttg aacatgaacc cagattgtgc gaagggcccg ggtatcaacc tgaaatttgc
     2521 gggcgtcgtc tttctggagg aagccttttc aaatcgccca gaggtggctc tttaccggcc
     2581 agaacagaca gtaccatagg accataatgc tctagcttcc agtccgtaag taaatccagg
     2641 cgggccagat cttccattgt agaagggcgc gcttccatga ttgcttcaag ctgcctgttg
     2701 gataaaatga tagaaggttc aaatccgcga tactctgcca catccttgcg ccattttttc
     2761 aaacgagcca gagcaggatc gggtggtttg cgtttctcac gaggcgaatc tgcattcgta
     2821 atctgcggcg atttgttgcg catttccaga attcgttccc cttctttgcg aacaaaatct
     2881 ggatgaagtc caaaagtaga cagctcgctg atgctatcgg gcattctttc tgctacgttc
     2941 aaaagagcgt cattggaacc aagacgaaac ggagcaatgt tatctttacg cgcgcgtgat
     3001 tcgcgaatct caaggagcgc tttgaggaag ccgcgaccga tcggggaaag ttgaaaggca
     3061 ccgggcacgt gcttccatga atcatgatca taaatccttt caactggata cgattccttg
     3121 atgatgcgaa tcatgtcctc tgcgaattca tccgcaaacc cgaacttctc gatttcgacc
     3181 ttcatggcct tcatgatttc ttcgagatag atggtgtcgc gatgcgcgta ttccatttgc
     3241 gaagcagtga gcggtctttt cttccaattg gatttctgtt ctgttttttc tagatcgcgg
     3301 cctgtgtaat gcttcacgag agacacaagg gagcagctga caaaaccgag catcctgcaa
     3361 cccaccatga tatcgaatag attctcgaac ttgaatttgc gaagacgccg tagctctgaa
     3421 atatcagagg cggccgtgtg cattactttt acaatggaag ggtttgcgaa gatctctgcg
     3481 agaccgtcca ctgtgagatt ggccagagga tcgatgagat aattcttacc ggaagcgctg
     3541 atctgaatca aacagagttc agactcatat gtatagaagc tagaactctc cgtgtccaca
     3601 gaaacgtact gtgacctgcc cattacagag actgccagat ccagatcctg ctgtttatca
     3661 atgaactggt agcccacaat gccagagtac tgatgagcgg tcctctgtca aattttgatt
     3721 ggtaaacaaa caagccacac gatccttgca catggccctt cgcaacagag accttgtgcc
     3781 agaaaagcgc gctcctgtaa aaaaatcagt gatcgtggcg ggtctagtgg cagcggccat
     3841 tctggcctta atgctgcggc tcgtcgtgca tgctgtgtgg atttttcctc ttcgcgtggg
     3901 cacggccagc atggagcctg ccgtaaaaaa aggacaaaca gtctacgttg tgaagcgcgt
     3961 agaactgcaa tttggcgata tcatattgta caaacacccg gataacccgg acgttcggct
     4021 cctgggaaga atcacaggtc tacccggaga tgcaatagaa atgaaagaaa agcaactctt
     4081 tagaaacggt gcaccagtcc aggatgcctg gcaaatacgc actcagacgg gctattcttt
     4141 acctggttcc gtatttccac gcgactcctt tgcagaaaga aagatcacta ccggcaaata
     4201 ctttgtgtta tgcgacctgc gcacggagtg cctggactca agacttcttg gagaaatcag
     4261 ccgcgagatg atcgtcggca gaattcgacc agattgactc cttacagaat cgcagccatc
     4321 tgcgaaggat cggagaaaaa tccattcaga gtttcccccg aatatttttc gatcaggagt
     4381 tccgcataag cctccgtatg cggacgaaac gcttgtgacc cggcagagtc ctggatcatt
     4441 tctgatagag aaaaagcagc atacaagaga tcgcagaatt cagttcgatg agaatccacc
     4501 cagaaatcag actgcttttc gcaatcattg atccgcgaag aaatgagtgc cagcgcgcgc
     4561 gctgcaagag gaaaagatcc tccgttgcgt acggcctctg aaagcatttc tttaaacgca
     4621 ttgtaaacat ttggtctgcc atacgccttg agagcgtgtt cgcaaagaag aggatgcgtt
     4681 ccttcccagg tttcattgat gatagcgtca ttgtgcagac gcggcaaaat agaaaaatcg
     4741 cccaggatcc cgttgcctcc gagaaccaga atgccttcgt gagtcagatg agtggccaga
     4801 acagtggagg catacttcag gagaggagtc agcatctttg agagcacatg atccttcgcg
     4861 ttgagatcgt aattgcgcat aacggcaaac tcaacgacag tctgcagcac ctgcatgcgc
     4921 gcgagagtgc gaaggactgc tggaagttca atgatcttct tcccatacgc tgtccgtttt
     4981 tgagtgtaag cgcgcacttc cagataggat cttctcccat gacccacagc ccccgcagcg
     5041 acatgcaaac gcgaagtgtt gatcacatac ttgatgaggt ttgccagtcc gtgtgccgga
     5101 cggcccagct ccgttgcttc cacatcttcg tagattgtct ccactgtaat tttgccttta
     5161 gagccaatga tgtctttttt cctcaccagc ctgcatccgt tgagcgttcc atcgtctttg
     5221 attctactca ccagaaataa gccgatcgtg gaagagtttt caattcgagc cgtagtgacc
     5281 cagagatccc ctggattgct acaaaaccat ttttctccgt ttaagatcca gctaccgtta
     5341 gacgcttttc tcgccacagt gcgattggcc gcgacgttgg atcctcccac tctctctgtc
     5401 acatattgcc cggccataaa atgtgatcca cttccctgtc tggcaagaat ggggagatac
     5461 tttctttttt gctcttctgt gccgattttt tcgagcacca gaatcaggcc atctgtcatg
     5521 gccagaggac aggcaactcc gccttcgcca ttcatgttgg tcatgaaagc aagcatcatt
     5581 ctatgatgaa aactgaacgg aaatttccag tctgagtggt accccagatt cacgatgcca
     5641 tgttcaaagc aaattctgcg cgcttctctc tgctcgtgac tgtagcgcac ttcatctatt
     5701 ctgtttcctg tggcgtcata cggcacgatt tctccgtatt ttccttcttt atggcatgcc
     5761 tcgatcagat cgttcaaaac gccgccacac aaagctccat attctgtaag atgtttatga
     5821 atcgcgtcca aatgacctgc ggccaggtcc gcgccatatc gattcatcaa atgcacaaaa
     5881 ctggaggttt gcgaatagaa gttccaccct cgggttccgc tgtaaccagt caggtcaaaa
     5941 ggggcgagtg ctggattggt catatggaat tccttccgca ggcggggcca ggggcaagaa
     6001 atttacgggt ttcagttgac gagttcgtaa ccactcgttc ttcctattca tttgaatgca
     6061 gctgccagac gaaaagcgac tcgaattgcg cacgtcgcgt ctgctcctgg tgatcgggct
     6121 tgtctcatac gcgattttct gggtgatcat agacaggctg gggatgaggg atccgcagcc
     6181 gcttcgctac attgcggtga tgattctcat tgccgcggcg ctggccgcgt ccttcgtcag
     6241 caatttcgca aaaaagcatc ttcgcaagct ggtcatcatt ctgctctacg tcatcgcaag
     6301 ccatttcttc ctacttctct acacgttcag actcgcaccc gtgcagatga tcgacatgct
     6361 gatctttctt tccgctgtca cggccacatc cactttcatc tttgaatcgc gcgccgggct
     6421 cctttctttt ttactgtttt cgactgcggg cgcagctgtg gcagcctatg cagtcaaaga
     6481 cccgctcgtt gacatctatc agttttttgc tgccatcgcg gtgctcggat ttgttgggtt
     6541 tatggccatg gcttaccaga tgcgcatgct ggataaaatt cgcggaagcg aacaacaact
     6601 gcgcaacgcc attcagcaat ctcaccgtta cgacttcatt gtaaacacgt cccgcgaatt
     6661 catgactctc atcaatcgcg agtacaagta tgaggctgtc aatgatgcct actgcgaagc
     6721 tcatcacaaa ccacgcgaaa tactcatcgg cagaccggta acagaagtct ggggcggaga
     6781 tctattcgaa agagtgatcc gcggttatct ggacgagtgc ttcaaaggca acattgtaaa
     6841 ttatggagtt ggatttgaat ttccggctct cggcccgcgc ttctttgatg taacttacta
     6901 tccctatcgc ggcgcaggcg atgaaatcac tcatgcagtc gtcgtatcca gagaccatac
     6961 tgaaaagaaa attgcagaag acagactgat tcacgaagcg ttacacgatg cgctgacagg
     7021 attgcccaat cggcatttgc tcacagatag aattggacac gcgctaaaga agaaaacacg
     7081 cgaagatgat tttgatttcg cacttctgtt tctggacctg gatcgcttca agatagtaaa
     7141 cgacaccatg ggacatctgg caggagacaa actgatcatt gctgtggcgc gcgttcttga
     7201 gaaatgcaca agaccaggag atactgtggg gcgcctgggc ggagatgaat ttgttctatt
     7261 gcttgagaat acgaatcttc agggagcgga agccgttgca tcacgcattt tgcgagaact
     7321 cagccagcct ctcatgctcg gagattctga agtattcacg ggggcaagca ttggaattgc
     7381 accatacgtt tccggttaca ccaggtcgga agaaatgcta cgcgatgcag acactgcgat
     7441 gtacagatcc aaggcactcg gaagatcccg ttatacgatc ttcgacgaag aaatgcgctt
     7501 tgcaatcaaa gaacaactca ccctggaagc ggaaatgcgt cacgggttgg aacgcggtga
     7561 atttacggta tatttccagc ccatttttaa actgacggat ggaacactga cggggtatga
     7621 gactctactg agatggaaac actcgcgaag aggagttctt ggaccttctg tttttctagc
     7681 ggtcgcagaa gaagcgaact tgattgatcc tctaggagaa ttcgtattgc gcgaagcatg
     7741 caggtttctt tcccgacttc cagggaaaga tacgttcgtt tccgtgaatg taagcgccaa
     7801 acaattccag cgacatggga tcctggaatc agtcattcag tctctcgcgg acttttcagc
     7861 ggaaccttcg cgtctcatgc tcgaactcaa agaagcaagc ttcgcgcgag gattcgatga
     7921 agtagcaagc atactgcgcg cgctctcgag acttggagtg aaacttgccg tagataattt
     7981 tggaacaggc aatgcttcta ttgtcaacct caagggactg cccctgcata tgctgaaaat
     8041 agacggctcc ttcgcacgcg gtctgcctca cgatgcaagt gatcgcgcac ttgtcgcggg
     8101 aatgatcagt atcgcgcaca gcctgggaat cgtggccggc gcaaccatgg tagaaactgc
     8161 gtcgcaagaa acacttctgc gaagccttgg atgcgacact gcccagggat ttcacttcgc
     8221 agaaccggca gactcttctg cgtggctcag ccaggcgcgc tagcgcgtgt aaccaaaaaa
     8281 gactgctttc tccacgaaac cgaaattctg ccgggcgcga tccttgcttc taccatttgt
     8341 tagaatgtct gaaagatcgg cgtagcctcc cttccctgag agctctacga agaaagatcg
     8401 aaagaaatca aaacgcaacc cgaccatcgc agaaacgccg tatcccgaaa gatgatacac
     8461 attgtttctg ccttcgccaa aaagtctaac gtcgctacga caaactatag gaccgccacc
     8521 aatcgaagag agcatgctca agccatgctc tccactgtcc gacatccaga tttgctgaat
     8581 gaatccaaca tccacctgaa aatagttcag tccatccgta tgatcaaatt tcaaaaagtc
     8641 cggagtgatg ttgattggct ctcccagatg cactccttcg tattgcttgt actgcgcggg
     8701 aaatagatag agatagggga gagattcaag agaaggctgc agatgcgcgc gctgaatcgc
     8761 aagaggatct atatacccgt agatattgga agcctgtccg cgcgtgagaa cgtacttcat
     8821 gtgatcctga ccaaatgaaa gagtaaagtg attggtgatg tatcgcgtgt agcgaaagct
     8881 aaactgaggc acgtcaaatt cgcttggact gagatagact ggcccgatgt tctctggttt
     8941 gtcttttgcc acaacgtctt ttaaagtgaa acgatacccc ggtccgcgaa agttgatatc
     9001 actctgagtg tatgcgtcgc ggttgtaacc ccactgaata ctccatgttc cttttctctc
     9061 atcaatcgag cgcggtccgg gtggaagtgg agagatgacc gggtccagag tcaatggcat
     9121 gagaggagtt tcctgagcgg aaagagtgcc gcaaacaacc agtgacaaaa cagagctaaa
     9181 gaggattgct ttcaagaggc caggattcca gtggattgtt gataaggaaa tcgttttcac
     9241 attcaatcgt tcccagtttt tctctgattc acttgccata tcgaggattc tgcccaaaat
     9301 ttggtcgtgc gtcgtatctt tcagatcatc gctgccgtta ctcttgcctg cccgcttcat
     9361 gctgagtcga gtgagaatga gatgcagagt tacttccttt caggcgcagc tgcagcttct
     9421 ggaattctcg aaggtggcgt gattgccccg gcatggcacg catatatccg cgatgttccc
     9481 cgcatcgacc gcgtgcagtc cctcatcttg tttagttctc tcgatcgccc tggcgccgcg
     9541 ctcactaccg aaattttcag acaggagtct cagacggact acagacatgc agccaacctg
     9601 cgcctccagg catacggcgg aactctggct ctctttggtc tggccggagt gcttgccacg
     9661 aagcctgtgc cgtcctggga tcatcctgcc gtgccggttc tgggcggtgt gtctgcaggt
     9721 atgtccttgc gatggctcgt tcagtggaat agatattcta acgttatgcg gcaactatct
     9781 acgaatcaac gtacgacaca gatcgctctc cttgcagccg ctccctccga caggctcact
     9841 ctggtttctt attacttacg ccagacagaa aaaaagacgt tcgctcgtac gcgaaggctg
     9901 gagagtgcat gcgcctcggt agattttgca ctgggggccg cggcattttt ttatgcttat
     9961 gcttccacga atcctgcacc ctcgacttcg gcctttttct tcgcaggtcc cgatcgagcg
    10021 ggctttcaaa tcagattcta atgcaactgc aatcagaagg tctggataga ccatactggt
    10081 tccgttcaag tcccccataa aacaatactc gattttgtcg cccggctttt acttgacaaa
    10141 aaaatagccg tcatatatgt agctatcgtg aatctgcctc tcacgtccat ttcgccgcca
    10201 agactcagac gcatttccct tctgacagga gtttcgggta tatacgccct ctctcattcc
    10261 tggatcagca gactggccct ccttgggctt cccatcactg tcatggattg cgcagatcgg
    10321 tttgatccat ttgcaattgc tgaagaagcc gcgtcgcagg atgtaaaacc agatgtgttg
    10381 cttgagacca tcacctgcca gaaaatcgca acacctctgc agttcctcgg cgctgcacgt
    10441 ggaatcctga atgcaaggag aggcggagtt gctgtgattc tttctccgct tcgccatttc
    10501 ctggacatag gggaaagcgc gcatgggcgc gagcttcttc tggatggatt tttgtcggtt
    10561 ctttcagagc tgagcgttcg ctcacgcccc gtacttctag tagaggcgca gcaggacgca
    10621 ccgctctttc attcagcatt cgatcgaatg gcagcacttg cttctcccat ctggcgaatt
    10681 ttgccggaca gcgtggaggt agcagagaac aagaaaacgc aaaccactcg ttccgtggat
    10741 cgaatggcgc aaaataatct cgaagggatt tcgcctgacc gactggctac cggttaactc
    10801 tcatgcctgc caatgaggcc cacgcgattt ccaccgaggg ctttcacctg gcgcacagct
    10861 ctttcagccg cactctctat ctccgcagat gtcgcataac gtggaaacca ggccactcca
    10921 caggagaatg tagcatagaa atcgccctcg cttgaagtgt gacgaatctg cgcgaagtcg
    10981 atgcgaatct cattgaacac ccgggcagca gtagctgcgt ccatggagtt cactatgaca
    11041 ccaaactcct cccctccgag tctgcccacg aggtccttac tccacactct ctgcttgagt
    11101 agacgaccca gactctttag aactgtatct cccaccgggt gaccgtatgt gtcgttgatc
    11161 aacttgaacc tgtcaatgtc taccatcaca aacggcaact ctactcctct tgctcctgcc
    11221 agctccaatt tcatttccag agagtctagc atcgcccgat ggttcaatag accggtcaag
    11281 ccatctcgga agtagagaga tttgatggat cgagttgctt ccactctaga agcaacgatc
    11341 aatgcaaggt agtctggatg gatcggaaga gtcaggcaat catcgccccc ctttgcaaga
    11401 gcggccattt gccttgctgg atcgagctcg ctggacaaaa atacaatggg aattcgttca
    11461 taccgcggat cctgtctgag aactgtggcc agctccattc ccaggatttc cggatagtaa
    11521 agattcatta gtatcaattc cggatgaaac aagtctcctg ctcgcagcat ttcagccgga
    11581 ttggagacca catgcgtttc cattcccgca gcccccagaa tcctgacaag gtgctgcgag
    11641 agctcaccat cgctttccac gatcaggatc cgatacgcct ggactacaaa cggagacgtt
    11701 aggcgatcca gcagagtaac gagagcatcc acgttcacag gcttagagaa aaaaccgctt
    11761 cccccaaacc ttaccgaccg aagacgcatt tgcatctcgt cgcttcctcc gagaaacaca
    11821 acac
//
LOCUS       DAOQJL010000046        11587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2806_length_11587_cov_5.010406, whole genome shotgun sequence.
ACCESSION   DAOQJL010000046 DAOQJL010000000
VERSION     DAOQJL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_11587_cov_5.010406"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..584)
                     /locus_tag="PLG78_03395"
     CDS             complement(30..584)
                     /locus_tag="PLG78_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22182.1"
                     /translation="MAENPRLIAALDFILNHATGAELEALASALSRRGNSSASSLNFQ
                     DMASSVTRGLSGRFAMPENMNEVTRNMVKGMIRQHEPNLSPEQIDVLLDQWVPSPQQV
                     RQGQEGKYPPEVLYSMAEQFLMYGIGNMPDSEARELKEAMPDWHEKYWNIFSPVIRTL
                     LADAIRGNLGLSECRARLKAHLRL"
     gene            640..1230
                     /locus_tag="PLG78_03400"
     CDS             640..1230
                     /locus_tag="PLG78_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE22183.1"
                     /translation="MHNLVRDHIERSIDVKKMLLEKETDRISAAGQALAELMQKGGRL
                     FTCGNGGSAGDAQHIATELLIRYRSAPERRSLPAISLVGDAAAVTAAGNDFGFEYVFS
                     RQIEGLGSQGDAILAISTSGNSANIIQALHAARKKHMMTFVLTGGSGGQIAKEGNLAD
                     HIIVVPTAETARIQECHIMIGQIFCAIIERQLFGFE"
     gene            complement(1259..2065)
                     /locus_tag="PLG78_03405"
     CDS             complement(1259..2065)
                     /locus_tag="PLG78_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22184.1"
                     /translation="MRHSLIVLLSAILVGACSTTSVQMQSPANGTRTVLASPQNSCQA
                     TTRGQWYLLFGALPINKVDLSLQETGKTYRVKEESKWYDVLIDVFGGAILTLTRKTIT
                     IDSCDERLIVVSQDELETERKKELQAALDKYATETPTLPEEAIFILSSGDSIRGKILE
                     LDSDTITIAVRSTVEQASQVDSVNAASIILRDGKTVVGEVIGQDNQSISLKVGGRTVV
                     IQKERIKRISYQRASTEKEGKPETQVEEVVLPRAQIKRIVLNRPPNGAVN"
     gene            complement(2119..4191)
                     /gene="pbpC"
                     /locus_tag="PLG78_03410"
     CDS             complement(2119..4191)
                     /gene="pbpC"
                     /locus_tag="PLG78_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNE22185.1"
                     /translation="MRRYSLLLALSAIGPLAGLQAVPSFEEVRSSYKSSEAVYLDRRG
                     RILQSIRLDESGRRLAWTSLDRISPALLSAVVRSEDKRFYSHSGADYLAILKAAFGRI
                     AKDTRRGASTITMQLASRLDDSLTPSSHGRTYEQKWRQIQAAREIEKTWSKSQILEAY
                     LNLVPFRGELEGISAASFGLFGKDPHALGDTESVILAALIRGPQASAEDVLRRSCMLA
                     YSMKLSYDCSQIKPDVARAFEGSHAIRPTASYAPHLPALIKSGESEIRTTLDLDIQLA
                     VREILHRHILSVRNRNVRDGAVLVLDNRTGEVIAYVGSTGDLSLAPLVDSIRSRRQAG
                     SALKPFVYAVGIDKRLITASTLLDDTPLNMPVFSGVYHPENYDRDYFGPVAARIALAS
                     SRNIPAVRLVSLLGVDSVLDVFRKMEFKNLEEADFYGPSIALGSMDVTLWQLTSAYRM
                     FASGGEWSSPFVERTSAAPAKRRIVSEAAAFIIGDILSDRESRSLTFGLENVLSTPFW
                     TAVKTGTSKDMRDNWCVGYSDRYTVGVWVGNASGEPMWNVLGVTGAAPVWQEVMRVLH
                     ADSPSRAPGLPDSVAIQATEYGKEYYIKGTEPAHFHEIEPRDPRILYPESGTIFALDP
                     EIPPERQRIFFRAASVQSRLQWKLDGKVIGEQNPAVWKPARGSHKLTLLLCEHVCAET
                     QSVGFMVR"
     gene            complement(4188..4880)
                     /locus_tag="PLG78_03415"
     CDS             complement(4188..4880)
                     /locus_tag="PLG78_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22186.1"
                     /translation="MADNIISVESLVKSFGDPPVQILKSLTFDIPRRQFVAITGRSGS
                     GKSTLLYTVSGLDDVTSGRVQMLGSDMHSAKPGDIEKMRNTQIGFVFQFHYLLPELTG
                     MENVLMPARKVRKDKEYKPWAIELMKRFDLGGCEDKLPSQMSGGQQQRVALARALIMK
                     PAVLFADEPTGNLDSVNAESVLEIFKEANENFGTTIIMVTHDEDYAARAHRRIHLVDG
                     KIDKDTLRRGKA"
     gene            complement(4894..6135)
                     /locus_tag="PLG78_03420"
     CDS             complement(4894..6135)
                     /locus_tag="PLG78_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE22187.1"
                     /translation="MLFLALKQLRARPRQTILTFIAIILGSTGYVVFSGLMLGFQDRI
                     LDSLINNDAHIKISARDQVIDRETFREVFFTDGGLKWAVPPSGKTDSTKLNGAQIWYQ
                     RLSQDPRVEAFEPQLSRQVIYVRGRFTVPGNLNGVVPTKHLKLTNIEKSIVNGSLMDL
                     NRGGAMVILGQELLNRLGARVGETIHVVASDGTKHPVKIVGIVRLGSRMIEATLGFAS
                     LETVQNVSGSAGEVSVVGVRLKDVSLAESVAQEWANYTNDKVESWQKANESILSVFFT
                     QDLVRNITTLTIILVVSFGIYNILNMVVNQKKKEIAILRSIGYSRSDVVVLFLYQGLL
                     LGFTGAVLGLVIGYLICQYLSTITLPGNQPIGFNKLLISFKYQIYIRAFLISCGASVL
                     ASYFPARAAGRLAPIEVIRQS"
     gene            complement(6137..6916)
                     /locus_tag="PLG78_03425"
     CDS             complement(6137..6916)
                     /locus_tag="PLG78_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE22188.1"
                     /translation="MRELLIKVNRKWLALLLLPVAVVVVYFLFFRNGATVKPKRGPVV
                     VAVYSLGTVTADNVFSFKAGLTASIQDIYVREGDAVGRGQKLLLLDSGAVVSAPFGGT
                     ITKVAFQKGEIVMPGVPVLTLIDLKNRHILVSLDQESALRVKPGQKVQISFETMRGQK
                     FSGTVERLYPQNGQFIARIKSELPADALPEMTADVAIETERKENSILIPAETIVESHV
                     MRVRNHVRQKVKVETGAADGEWIEITNNVIEDNDEILIQKK"
     gene            complement(7036..7473)
                     /locus_tag="PLG78_03430"
     CDS             complement(7036..7473)
                     /locus_tag="PLG78_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE22189.1"
                     /translation="MRSWTSEASFIAGQRTDVERIREIMNEPYRIILVTQVRVGIAGC
                     VSVDVSERLGHFGMLSVGVPFQKGGMAKALIRAAESWAADQGAREMRLQVLMNRPELI
                     SYYERQGYRMTEERIPFPNGEPRFGEPLIDGLMFGTMSRPISD"
     gene            complement(7569..7985)
                     /locus_tag="PLG78_03435"
     CDS             complement(7569..7985)
                     /locus_tag="PLG78_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE22190.1"
                     /translation="MAGGVGGGGDDNPIADINITPFVDIVLVILIIFMITAPMLYQST
                     IDVNLPKADSGQQSEKSTLTFTINKEGELKWDKEKVKWEDVPGKLKALGGSLSTKTAI
                     ITADTATSHGTVVKLMDILRKSGVTKVGITVEGQGR"
     gene            complement(7998..8666)
                     /locus_tag="PLG78_03440"
     CDS             complement(7998..8666)
                     /locus_tag="PLG78_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE22191.1"
                     /translation="MINAVFKAFTVLGSEWVLVILLLLSVLSIAMTLERWQFYRKAQS
                     GIVAFRSSLRTLIVEAKWKESLALVEARIAAKKGKYPDLETDMAYTLLTHTKAAGGQA
                     VSPASMNQLAQDSLTRSRIAWDKYLTVLASIGSNTPFFGLFGTVLGIMKAFADLGTGG
                     AAGAQGATQVSAGIAEALVATAIGLFVAIPAVVSYNTFQKRVKTAAMEADALKSFLIG
                     SMMK"
     gene            8764..9540
                     /gene="lpxA"
                     /locus_tag="PLG78_03445"
     CDS             8764..9540
                     /gene="lpxA"
                     /locus_tag="PLG78_03445"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNE22192.1"
                     /translation="MKIHPTAIIHPTAKLHESVEVGPFCIIDQDVVMGEGCFVESTVK
                     IHARTVIGKNNRFHHGAIIGCEPQDLGYDRSSPCYVKIGDNNVFKEGANIHRGTKPDH
                     PTLIGNNNYFMGNFHVGHDCILGDHSIFTHGTVLAGHVTVGNYAFISGVAAVHQFCKV
                     GDYSMIAGCSKIVKDVPPYATADGNPATLIGINSVGLKRAGYSPAKRDAIKQAYKLLF
                     HSGKNIKQALAELKSQENTDEVKLIVKFFEDSDRGVTDHR"
     gene            9537..10103
                     /locus_tag="PLG78_03450"
     CDS             9537..10103
                     /locus_tag="PLG78_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22193.1"
                     /translation="MKRFAVLLLLFPAILIAEDVKAKLGIELKRGTASSKAHARETVR
                     SGDRITAYVLPAQNSNVYVVSIKDGKASLINGKGIALSSSEAAAIPSMESPIILTEKG
                     LVEIQIYVSREPISDLNELANGVTVSRFREIERRIKDRAGITGAPEKPAPVAANVRAM
                     GADVIAREMPGYSSPKIILAKFYFDVQN"
     gene            10090..>11587
                     /locus_tag="PLG78_03455"
     CDS             10090..>11587
                     /locus_tag="PLG78_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNE22194.1"
                     /translation="MFKTEPDRSASLILSSLWTILGLLLIAVIGTWHRTDERLNDWIA
                     RVAVHRGFAPASSPRICYLFITDTTYARFNKQALNREQLAHANDILADLGAESIVYDL
                     ILARPGEQSADAAFARSLERTKIVFLPSGFAARKRKPVDPDALEVRESGRGGAPIALH
                     EIASLPDFAQRARGGGHVFAPSDSDGVYRRMNLFVQEGRRIIPGLALSVLLFQRHQTL
                     ADIEIHWEDEVRIPATASAPELIIPIDSAGATRIAYTAKWGRDFESMTIDDLIAGYED
                     PDKRGNLINMFEGRTVFIGDTSTGIADAGATPLENDVPLVAMHAALLSSMMDQHSFGV
                     WKPVHVWALFTACCILLVFSSTLRRAGSLHITAVLLLIALWILPFLGYYRYTLFPGAT
                     VLAVFVLVYLGTTVSIQVRVWKQNVIIRAENSFLHRDLSIAANIQSKMLPAALPDRSD
                     LEIAAVNIQAQSIGGDFYDVITLPSGKVLLLAGDVSGKGIPAALNMSGI"
BASE COUNT         2908 a         3088 c         2784 g         2807 t
ORIGIN      
        1 ctcacagaat cgcctaacgt ctgcggcttt cagagccgca gatgagcttt caaccttgca
       61 cggcattcac tcaacccgag attccctcgg atcgcatccg ccagaagcgt tcgaatcacg
      121 gggctgaata tgttccagta tttctcatgc cagtccggca ttgcttcttt caactccctt
      181 gcttctgaat caggcatatt gccaatacca tacatgagaa attgctccgc catcgagtag
      241 agaacctcgg gtggatattt tccttcttgt ccctggcgca cttgctgcgg actgggtacc
      301 cattgatcca gcaagacgtc aatctgttct ggcgaaagat ttggttcatg ctggcgaatc
      361 atgcctttca ccatgttgcg cgtcacttcg ttcatgttct ccggcatcgc aaatcttccg
      421 gaaagccctc gcgtaacgct gctcgccatg tcctgaaaat ttagactcga tgcgctggaa
      481 ttcccgcggc gcgaaagtgc tgacgcaagc gcttctaatt ccgcgcctgt agcatgatta
      541 agaataaaat ccagagccgc aatcaatctt ggattttctg ccataagccc tcaggtccga
      601 attgattgac gagactttct gcttcgaaag acttccccaa tgcacaacct tgtccgggat
      661 catatagaac gcagtattga tgtcaagaag atgctgctag aaaaagaaac ggacagaatc
      721 tcagctgcgg ggcaggcact cgcagaacta atgcagaaag gcgggcgtct ttttacatgt
      781 ggaaacggtg gaagcgcggg cgatgcccag cacatcgcca cggaattgct catccgctac
      841 cgttccgctc ccgaaagaag atcgcttccg gcaatttccc ttgtagggga tgcagcagct
      901 gtcacggcag ccggcaatga ctttggtttt gagtatgtat tttcgcggca gatcgaaggt
      961 ctaggcagcc agggggacgc gattctcgcc ataagcactt ccggcaattc agcaaatatc
     1021 attcaagctc ttcacgccgc acgcaagaag catatgatga cgtttgtact cactggtgga
     1081 agcggtggtc aaattgcaaa agagggaaac ctggctgatc atatcattgt ggttccaacc
     1141 gcagaaaccg cacgcatcca ggaatgccac atcatgatcg gtcagatctt ctgtgcgatc
     1201 attgaacgcc agttgtttgg attcgaatag aacattcact acaaccttca gccaggcttt
     1261 agttcactgc cccgttaggt ggtcgattca gaacgattcg ctttatttgt gctcgcggaa
     1321 gcaccacttc ctccacctga gtctcaggtt tgccttcctt ctccgtggat gctcgctgat
     1381 aagagattct cttgattctt tctttctgaa tcacgactgt gcggccgcca accttcaggc
     1441 taatgctctg attgtcctgg ccgatcactt cacccaccac cgtttttcca tctcgtagaa
     1501 taatgctggc cgcgttcacc gaatcgacct gggacgcctg ctccactgtg cttcgcactg
     1561 cgatggtgat tgtatccgaa tcgagctcca gaatctttcc gcgaatcgaa tcaccagagc
     1621 tcagaatgaa aattgcctct tccggaagtg taggagtttc cgttgcatat ttgtccagag
     1681 ctgcctgcag ttccttcttg cgctccgtct ccagttcatc ttgagaaacg acaatgagcc
     1741 tttcgtcaca ggaatcgatc gtgattgtct ttcgcgtaag ggtgagaatg gcgcccccga
     1801 agacatcgat gagcacatca taccactttg actcttcctt cactctgtat gtcttacccg
     1861 tttcttgcag ggaaagatct actttgttga tcggtagcgc gccaaacaac aagtaccact
     1921 gtccgcgcgt cgtcgcctga cagctgtttt gcggagacgc cagcacggtt cgcgtgccgt
     1981 tagccgggct ctgcatttgc acagatgttg ttgaacatgc cccaaccaga attgcggaaa
     2041 ggaggacgat caaagagtgc ctcatgagtt gattcaaaag ctggcgcccc ctcaccgcaa
     2101 gcagtaaatt ccaagggatc aacgaaccat gaatcctacg gactgagtct ctgcgcagac
     2161 gtgttcgcat aacaataatg ttagcttatg actacccctc gccggtttcc agacagcagg
     2221 attctgctca ccaatcactt tgccgtccaa tttccattga agtctgcttt gcacggaagc
     2281 cgctcgaaaa aatatcctct gcctttctgg cggaatttcc ggatcaaggg cgaatatcgt
     2341 tcctgattca ggatacaaaa tgcgaggatc acgcggttcg atctcatgaa aatgggctgg
     2401 ttctgtgcct ttgatgtagt attctttccc gtattccgtt gcctggattg caaccgagtc
     2461 cgggagaccg ggcgcgcggg acggagaatc tgcatggagc acccgcataa cttcctgcca
     2521 gactggcgca gcaccggtga ctcccagtac attccacatg ggttcgccgc ttgcgttacc
     2581 aacccataca ccgaccgtgt aacggtcgct atagccaacg caccagttat ctctcatgtc
     2641 cttgcttgtg ccggttttca cggcagtcca gaagggagtc gaaagaacgt tttccaggcc
     2701 aaacgtcaga cttctgcttt cacgatccga cagaatatct cctataatga aggccgcggc
     2761 ctccgataca atcctacgct ttgcgggcgc ggcactcgtt cgttccacga aaggagaaga
     2821 ccattcgcca ccggacgcaa acattctgta ggcagacgtt agttgccata gagttacatc
     2881 catgcttccg agagcaatcg aaggcccata aaaatccgct tcctcaaggt ttttgaattc
     2941 cattttgcga aagacatcca ggacggaatc cacgccgagc aaagatacca gacgaacggc
     3001 aggaatgtta cgcgaagaag ccagagcgat gcgcgcggca acgggcccga aatagtcacg
     3061 atcataattc tcggggtgat acactccgga aaaaacaggc atattcagag gcgtatcatc
     3121 cagcagagtg gaagcagtga tgagacgttt gtcgattccc actgcataca caaatggttt
     3181 gagagcagaa cccgcctgcc ttcgcgagcg aatggaatct acgagaggcg caagagaaag
     3241 atcccccgtc gagccaacgt aggctatcac ttcacccgta cgattgtcca gaaccagaac
     3301 cgcaccatcg cggacatttc gatttcgcac ggaaagaatg tgccgatgca aaatttctcg
     3361 caccgcaagt tgaatatcca gatccagagt ggtgcgaatt tcagattctc cggatttgat
     3421 cagagcgggg agatgaggag cgtaggaagc agtcggtcga atcgcatgcg aaccttcgaa
     3481 tgcgcgtgcc acgtccggtt tgatttgcga acaatcgtac gaaagcttca tggaataggc
     3541 aagcatacaa gaacgacgca gtacatcctc tgcagaggcc tgaggaccac gaatgagggc
     3601 cgcgagaatg acggattcag tatctccgag agcgtgcgga tcctttccaa acaatccaaa
     3661 agaagcggcg gaaatgcctt ccagctctcc gcgaaagggg accagattca agtaggcctc
     3721 aaggatttgt gatttgctcc aggtcttctc gatttctctg gccgcctgga tttgcctcca
     3781 cttttgttcg taggttcttc cgtgcgaaga aggggtcaga gaatcgtcga ggcgtgaagc
     3841 gagctgcatg gtgattgtgc tcgctccgcg tcttgtgtct tttgcgattc ttccaaacgc
     3901 ggctttcaaa atggcaagat agtccgcgcc ggaatgcgaa tagaaccgct tgtcttcgct
     3961 gcgcacaact gcgctgagta aggctggaga tatccgatcg agagaagtcc aggcaagcct
     4021 gcgacccgac tcatccaggc gaatgctctg cagaattcta cctctcctgt ccaggtacac
     4081 ggcttcggaa gatttgtagg aggatcgaac ttcttcaaac gaaggaacgg cctgcaatcc
     4141 ggcaagaggg ccaatcgcag aaagtgcaag caagagcgaa taacgtctca tgcttttcca
     4201 cggcgaagag tatccttatc aattttgccg tccaccagat gaattcttct gtgcgcccgc
     4261 gcggcatagt cttcatcgtg agtgaccatg atgatggttg ttccgaagtt ctcgtttgct
     4321 tctttaaaga tttcaagcac actttccgcg ttcactgaat cgagatttcc agtgggctca
     4381 tctgcgaaaa gaacggcagg cttcatgatc aaagcccgcg caagagcgac gcgctgttgc
     4441 tggccaccgg acatctggga cggaagttta tcttcgcagc ccccgagatc aaaacgtttc
     4501 atgagttcaa tagcccacgg tttatactct ttgtctttgc gaaccttgcg cgcaggcata
     4561 agcacattct ccatacccgt caattccggc agcagatagt gaaactggaa tacaaagcca
     4621 atctgcgtgt tgcgcatctt ttcaatgtca cctggttttg cactgtgcat atcggagccg
     4681 agcatttgca cgcgaccaga cgtaacatcg tcaaggccac tcacagtata aagtaaagta
     4741 gacttcccgg aaccggaccg gccggtgatg gcaacgaact gacgtcttgg aatatcaaac
     4801 gtaagcgatt ttagaatctg cacgggcggg tcaccgaatg atttaaccag agactcgacg
     4861 gatatgatgt tgtctgccat caccattccc ccgtcacgat tgacgaataa cctcgatcgg
     4921 tgccagacgc cctgcagccc ttgccggaaa atagctggcg agcacgctgg cgccacagga
     4981 gatcaagaac gctctgatgt agatctgata cttaaaggat attagtaatt tattaaaacc
     5041 gattggctga tttcccggaa gtgtaatcgt agatagatac tggcaaatca aatagccgat
     5101 gacgagtccc aggaccgctc ctgtgaatcc cagcaaaagt ccctgataca agaacaacac
     5161 cacaacatcc gagcgagagt aaccgatgct tcgcagaatc gcgatctcct ttttcttctg
     5221 attcacgacc atgttgagaa tgttgtaaat tccgaaagat acgaccagga tgatcgtcag
     5281 agtggtgatg tttcgcacga gatcttgtgt aaaaaaaaca gaaagaatcg actcatttgc
     5341 cttttgccag ctttcgactt tgtcgtttgt atagttggcc cattcctgcg cgacggattc
     5401 tgcaagactt acatctttca atcgcactcc aaccacggaa acttctccag cagatcctga
     5461 gacattctgt acagtctcaa gagatgcaaa acccagagta gcttcaatca ttctggaccc
     5521 cagacgaaca attccaacaa tcttcaccgg gtgtttggtt ccatcagatg caaccacgtg
     5581 gatcgtttca cccactcgcg cgcccagacg attcaaaagt tcctggccaa gaatcaccat
     5641 ggctccgccg cgatttagat ccatcagaga tccgttcacg atagacttct caatattcgt
     5701 gagcttcaag tgcttggtgg gaaccacgcc atttagattt cctggaacag tgaatcgacc
     5761 tctcacataa atgacctgac gcgaaagctg gggttcaaac gcttccactc tagggtcctg
     5821 gctcaatctc tggtaccaga tctgagctcc gttcaacttc gtgctgtctg tcttgccgga
     5881 cggcgggacc gcccatttta gaccaccgtc tgtaaagaaa acttcgcgaa aggtctcgcg
     5941 atcgatcacc tgatcacgcg ctgaaatctt tatgtgagca tcgttgttga tgagactgtc
     6001 tagaatgcga tcctgaaatc caagcataag cccggaaaac actacatagc ctgttgatcc
     6061 tagaatgatg gcgatgaaag taagaatggt ctggcgcggc cgagccctga gttgtttgag
     6121 tgcgagaaag agcatttcat ttcttctgga tcagaatttc atcgttgtct tcgatcacgt
     6181 tattcgtaat ctcgatccac tctccgtctg ccgctccggt ttctactttc actttttgtc
     6241 tgacatgatt gcgaacgcgc ataacgtgag attccacgat cgtttcagcc ggaattagaa
     6301 ttgaattctc ttttctctct gtttcaatcg ccacgtctgc agtcatttca ggaagcgcat
     6361 ccgctggaag ttccgacttg atgcgagcaa tgaactgccc attctgcgga tagagcctct
     6421 cgacagtgcc tgagaacttt tgacctctca tggtctcaaa gctgatttga actttctgcc
     6481 ccggcttcac gcgaagcgca gattcctgat ccagagaaac caggatatgg cgattcttca
     6541 aatctatgag agtcaaaacc ggaacgccag gcatcacgat ttcgcctttc tgaaaagcaa
     6601 ctttcgtgat ggttccgcca aacggagcgg agacgactgc gccagaatca agcaagagca
     6661 acttctgacc gcgaccgact gcatcgcctt cgcgaacgta aatgtcctga atgctcgcag
     6721 tcaatcctgc cttaaaagaa aacacattgt ctgccgtcac cgttcccaga gaataaaccg
     6781 ccacaacgac agggcctctc tttggtttca cggtagcgcc attgcggaag aacaagaagt
     6841 agacgaccac gacggcgacg ggcagaagaa gaagcgccag ccatttccga ttcactttga
     6901 tcagcaattc acgcatttcg ttcctctttt caatacccgc gagcctgcct gcagctgtca
     6961 agatgactca actcgattct ggcgatgaat gatatgattt ttgttagacc cggaaatcca
     7021 gattctgttc caaaatcaat cagagatagg acgactcatc gtgccgaaca taaggccgtc
     7081 aatgagaggt tccccgaaac gcggttctcc atttggaaat ggaattcttt cctccgtcat
     7141 gcggtaaccc tgcctttcgt agtacgaaat caattcgggg cgattcatca atacctgcag
     7201 tctcatctca cgggcaccct gatcggcagc ccaggattct gccgcgcgaa tcagcgcttt
     7261 ggccattcca cctttctgaa aaggaactcc aacactgagc ataccaaaat gaccaagtct
     7321 ttcgctgaca tccacggaaa cgcaaccagc aatgccaacg cgcacctgag taaccagaat
     7381 gatcctgtaa ggttcattca tgatttcgcg aattcgctct acgtctgttc gctgccctgc
     7441 aatgaaggac gcttcgctcg tccatgagcg caaaccggtg gttccgcgat agactgaatt
     7501 tacaagatca gagatgcgtt gcgcttctac tgcgcgggca gggcgaatgc tccagtctgc
     7561 cagatctttt acctgccctg accttctacg gtgatgccta ctttcgtaac gcccgatttg
     7621 cgcagtatat ccatgagttt aacaacagtt ccgtgagaag tcgcagtgtc cgctgtgatg
     7681 atcgcggtct tcgtgctcaa agagcctccg agcgctttga gtttacccgg tacgtcttcc
     7741 cacttaactt tttctttatc ccacttgagt tcaccttcct tgttgatagt gaacgtgaga
     7801 gtagactttt cactctgctg tccgctatct gctttgggaa ggtttacgtc gatcgtgctt
     7861 tgatacagca tgggcgctgt gatcatgaag ataatcagaa taactagaac gatatccacg
     7921 aaaggcgtaa tgttaatatc cgctatcgga ttgtcatcgc ctccgccgcc aactccacca
     7981 gccattgcct gaactcctta cttcatcatg cttccaatca ggaagctttt gagtgcgtcc
     8041 gcttccatcg cggcagtctt cactcttttc tgaaacgtgt tgtaagagac aacagctgga
     8101 atcgcaacga acagaccaat cgcagttgca acgagtgctt ccgcaatgcc tgcactcact
     8161 tgagtggcac cctgagcacc cgcagcaccg cccgttccaa gatctgcgaa tgctttcata
     8221 atcccaagaa cggttccgaa cagtccaaag aatggcgtgt tacttccaat agaagccaga
     8281 acggtgagat acttatccca tgcgatacgc gatctggtca gagaatcttg cgctagttga
     8341 ttcatggacg caggcgaaac cgcctggcca ccggctgctt tggtgtgagt cagcaaagtg
     8401 taagccatgt ccgtttcgag atctggatac ttacctttct tggctgcaat ccgggcctct
     8461 acgagtgcaa gagattcctt ccacttggcc tcaacaatca atgtgcgaag gctggatcgg
     8521 aaagccacaa tgcccgactg ggctttgcga taaaactgcc acctttccag agtcatcgca
     8581 atggaaagaa cagatagtag caacagaatg accagaaccc actcagatcc cagtactgtg
     8641 aaggctttga aaactgcgtt aatcatatca gatgtgacca ggaaaacagc tggagctggc
     8701 ctggtctact ttgaaatttg gttgccctgc attccagggc acctccagat accaggataa
     8761 gatatgaaga ttcacccaac cgcgatcatt cacccaactg caaaacttca cgaaagcgtg
     8821 gaggtgggac cattctgcat catagaccag gacgtggtca tgggcgaagg ctgcttcgta
     8881 gaaagcaccg taaaaatcca cgctcgcacc gtcataggga agaacaaccg tttccatcat
     8941 ggtgccatca ttggctgcga gccgcaggac cttggatacg atcgctcctc tccctgctat
     9001 gtaaaaatcg gggataacaa cgtttttaaa gaaggagcca acatccaccg gggcacgaaa
     9061 ccggatcacc cgactctaat tggaaacaac aactacttca tgggcaattt tcatgtcggc
     9121 catgattgca tcctgggaga ccacagcatc ttcactcacg gcaccgtact ggccggtcat
     9181 gtaacggtcg gaaactacgc tttcatttcc ggtgtggccg cagttcacca gttctgcaaa
     9241 gtgggggact attccatgat cgctggatgt tccaagattg tgaaagatgt tccgccttac
     9301 gccacagcag atggaaaccc tgccacgctg attggaatca acagtgtcgg tctcaaacgc
     9361 gccggctatt ctccagccaa acgcgacgcg atcaaacagg cttacaaatt gctttttcat
     9421 tccggcaaga atatcaagca agcgctcgct gaattgaaga gccaggaaaa cacagacgaa
     9481 gtaaagctga tcgtgaagtt ctttgaggac agcgatcgcg gggtcacgga ccacagatga
     9541 aacgattcgc ggtgttactc ctcctgtttc ctgcgatcct gatcgcagaa gacgtcaaag
     9601 caaaactcgg aatcgaactc aaacgtggca ctgcttcttc caaagctcac gccagagaaa
     9661 ccgtacgctc aggtgatcgc ataacggcat atgttctccc tgctcagaac tcaaatgtat
     9721 acgtagtttc aatcaaagat ggaaaagcat ctttgatcaa tggaaagggg attgcgcttt
     9781 cttcctcgga agcggccgcc attccatcta tggaatcgcc aatcattctg acagaaaagg
     9841 gtcttgtaga aattcaaatc tatgtttctc gcgaaccgat ctctgaccta aacgagctag
     9901 caaacggagt caccgtgagt cgctttcgcg aaatcgaacg aagaatcaag gatagagcgg
     9961 gaatcacagg agcaccagaa aaaccagctc cggtggcggc taacgttcga gccatgggag
    10021 cagacgtgat tgcacgggaa atgccgggct actccagccc gaaaatcatt ctggcaaaat
    10081 tctatttcga tgttcaaaac tgagccggat cgatccgcgt ctctaattct ctcctcgctc
    10141 tggaccatac ttggcctgct tctcattgca gtcattggaa cctggcatcg cacggatgaa
    10201 agactgaacg attggattgc gagagttgcg gttcatcgtg gttttgctcc tgcttcttcg
    10261 cctcgcatat gttacttgtt catcacggat acaacgtacg cgcgcttcaa caagcaggcc
    10321 ctcaatcgcg agcaacttgc tcacgcaaac gacatactcg cagacctggg cgctgaatca
    10381 atcgtatacg atctcatcct ggcaagaccg ggagagcaga gtgcggacgc agctttcgca
    10441 cgctcattgg aaagaacaaa gatcgtattt cttccgtctg gattcgccgc acgcaagaga
    10501 aaacctgtag atcctgatgc cctggaagtg cgggaaagcg gaagaggtgg tgccccgatt
    10561 gcgcttcatg aaattgcatc gcttcctgac ttcgcgcaaa gggcccgcgg cggcggacat
    10621 gtttttgcgc cgtcggattc agacggtgtt tatagaagaa tgaatctgtt cgtccaggaa
    10681 ggccgccgga tcattccggg gctggctctt tctgtccttc tttttcagag acatcaaact
    10741 ctcgcggaca ttgaaataca ctgggaagat gaagtccgaa ttcctgcgac tgcgagcgca
    10801 ccagagctca taattccaat tgattccgcc ggcgctacac gaatcgccta caccgcgaaa
    10861 tggggacgcg attttgaaag catgacgata gacgatctga tcgccggata cgaagatcca
    10921 gataagcgcg gcaatttgat caacatgttc gaaggcagaa ctgtcttcat tggagacaca
    10981 agcactggca ttgcagatgc aggcgccact ccactcgaaa acgatgtgcc gctggtggcc
    11041 atgcatgcgg ctttactgag ctccatgatg gaccaacatt cattcggtgt gtggaagcca
    11101 gtgcatgtgt gggctctttt tactgcttgc tgtattcttc ttgttttttc ctccactttg
    11161 agacgtgcgg gttctttgca cataacagct gttttactcc ttatcgcact ctggatactt
    11221 ccattcctgg gatactaccg ttatacgctc tttcctggcg ccaccgtgct tgccgtgttc
    11281 gttcttgtat atctgggtac aacggtcagc attcaggtgc gcgtctggaa acaaaatgta
    11341 atcattcggg cagaaaatag ttttttgcat agagacctgt cgatcgcagc aaacatacag
    11401 agtaagatgc tcccggccgc tctcccggat cgatcggatc tggaaattgc tgcagtaaac
    11461 attcaggcgc agagtattgg cggcgatttc tacgatgtga tcactctgcc atccgggaag
    11521 gtgctgctcc tcgcaggaga tgtctccgga aagggaattc cagcagcgct caacatgagc
    11581 ggaatac
//
LOCUS       DAOQJL010000047        11522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2824_length_11522_cov_4.447807, whole genome shotgun sequence.
ACCESSION   DAOQJL010000047 DAOQJL010000000
VERSION     DAOQJL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_11522_cov_4.447807"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLG78_03460"
     CDS             complement(<1..258)
                     /locus_tag="PLG78_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22195.1"
                     /translation="MRLFLNAAFLVLTLPLVASCRLAELQQQPPVTPSQWCELRPCVT
                     LGTTVFNEPLGSFLVFLLAFLWVASGVYFLKNHDGQSSRKWL"
     gene            complement(332..1039)
                     /locus_tag="PLG78_03465"
     CDS             complement(332..1039)
                     /locus_tag="PLG78_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22196.1"
                     /translation="MKLSVTAEKAIQAYGGTALWNKARTIETILSANGLAFTLKRRPA
                     FERAKFTTEIHRPFARMTPIGKETGVTGVLDGADVRLENSSGEILEERKNARSYFPFG
                     RRLFYWDDLDMTYFANYATWNYLTLPALLMNDAITWTEKRPGFLEGIFPDSIPTHNRV
                     QTFEFDVQSGLLLQHNYAPAIISKFANAANVVKEHSQGDGALFTSHRIVTPQGFGGKP
                     MGGPVLIDLRIHDFQIR"
     gene            1167..1526
                     /locus_tag="PLG78_03470"
     CDS             1167..1526
                     /locus_tag="PLG78_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNE22197.1"
                     /translation="MITDQRFRQLALSFQDTTEVPHFDRAAFRTPKRIFATMGKDGVN
                     LMLDTETQAIAVKKHPRAFSVLQNAWGKKGATHVDLRAVSEAALVPILGIAYKRATVG
                     MRSAATRRKLRESKTKA"
     gene            complement(1527..2561)
                     /gene="lpxD"
                     /locus_tag="PLG78_03475"
     CDS             complement(1527..2561)
                     /gene="lpxD"
                     /locus_tag="PLG78_03475"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE22198.1"
                     /translation="MTLQELSQKLGMKCAGKADKEISGIKDLESPGEASPDHIYFVES
                     KKRLKKYSEKIAGVKVALTVPELAHNFEYAIVVDPPEMARLKFIELLGIFEKKSQPEK
                     GISPRASIHPTAKIGKDVSIMENVVVMKDCVIGDGCVLYPNVVVEERARVGAQSVLMA
                     GVVIGYNCSLGTNALIHANTVIGADGFGFYDKGGVRHKIPQIGNVIIGNHVEMGACCT
                     VDRATIESTLVGDYTKFDDHVHIGHNCRVGNYVFIAGATVLAGSVVLEDRVIIGGQSA
                     IAEHLRMRKGSVLLGMSALTADSQEGTIYFGIPALPAIVMHRIHALFPKLPELFRRLK
                     ESDAAEPSGR"
     gene            2867..4084
                     /locus_tag="PLG78_03480"
     CDS             2867..4084
                     /locus_tag="PLG78_03480"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22199.1"
                     /translation="MHEIVFRITTIIVAYIVLWTTLIELNIGIIYKENLRYLRLIIDS
                     CGLTIGILITGGASSPGIVTYAGTTGLASLYISKRFGVFSIATNVTLFNAMLFLVHEG
                     ILPRINVLAPDGLGSESTMAEIILANIMMILYSIILHLVIQPNYRNLHTERAELKARN
                     DAMHREITLARRIQEKLIPARSPDESIAFLYKPMDQVGGDFFDFIEHDGKIGIFLCDV
                     SGHGVPAALIASMMKTIVLQAGHRASDPATFMSYINETLQSRTAGNFVTAFYASFQKS
                     SRSIVYCNAGHCSPYVIDQTGVRELEGVKSVPLGLWSNDELRLRNRAFSNVEINLIAD
                     SRILFYTDGLTETRCENGDLMFEEGPLQENLVATRQQTSAAAVEDLHRALARFHGSNS
                     FEDDVCVICLDIK"
     gene            complement(4096..4497)
                     /locus_tag="PLG78_03485"
     CDS             complement(4096..4497)
                     /locus_tag="PLG78_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HNE22200.1"
                     /translation="MSAFRMLVTSVFVLFLSGCGVFADVGLNPSTVSGDEARSILQDR
                     LAGFFVKDISDQNATSLGLDFFIADLAQIKPGSNYWRRDVEHCASVIYLVALAIDQPQ
                     ITARTASSPDSRWPGPLLCSLKEVGTWDLKQ"
     gene            complement(4494..7556)
                     /locus_tag="PLG78_03490"
     CDS             complement(4494..7556)
                     /locus_tag="PLG78_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNE22201.1"
                     /translation="MKSFLSFLLIFTFVSISSLNAEDAVVVVVEFQPFDCPPNPGLAR
                     DLRTAVSGKLTGSGVVVEEAPSAVLNDALAQARQRKAAFVIHGYYNVSGGSVNIYGQI
                     YNPETGVVIDALNAVDESRQIEGLVLPEDETRTPTPRRIEDFSIRLANRILSNPRRTE
                     RRDSINEAILSQRIGKEIQFRLAQENVQSQADQAFKVISERDQIVVAVSKFAQKASEA
                     PADVQVINRSQIRRFGYRNLNEALNSIPQVYSHYVGQNWSADFRGLFINNQIERRVLY
                     LQDGKKLNDYFHFGEFYSDLYTDMDRIDRIEVIKGPGAALYGNNSITGVVNIVTRKPT
                     RENEIETSTDYDAGTGTFAVRALYLSKFSNDLSVTADVSHFEGTGVYRSGQPSWGSTR
                     YYDYRLGSSSQTSASGREDMPYYTGQRIWLATGSQVDNGSPFPNFNFDITYKDFSVKA
                     LYLSKRVSWVDPRTDGGVFGSQTEFGSAINDRIWGVGYAMLEYRPSYLEKYEPSIRVF
                     RQLGINSDFREKGFEGFAGNTAACCGTSATARLASSQYTQFMALNGGSMIKAYASTAY
                     ANGMEFQFTPFRMESSQNFVRKIHALVGGNGAEVNYINYQRIENQRNEVYLYNQGIGD
                     DGRQYGAFGQLTLGLASGTSAIAGMRYDYQKVNRVYRHEYGNAAYTAAENISNPITKT
                     SVPTDTNADFIPFFDPVTGQVRVAQYVRMLQGYRQPFQRKNVVAQDSTPRFALIQSIE
                     QTATTLKLIYSEAFRAVTPQELIRLPSDQGNAESEKVINREVNLIQDLFSGRVSVSLD
                     HFWLRGSTLYAFNAATATFGQSPAWSNTGGSVAISVSPDDLWKFGGSGTSYRLRRASD
                     VSFLDKIDTPSDQALSSPTRLYKANVSRGFFGNKATVAIEYYYNGPIYLMERPPATLQ
                     DVLDSNLELNALPPLPAELDRNSLSGKGNIRNYRIYKVPPSWFYNLTISSNLDSELFF
                     VFSIKNILNRVVLYPLDFDSESFSSPNFAPHQLRGFGREFFLKAGYRF"
     gene            complement(7553..9100)
                     /locus_tag="PLG78_03495"
     CDS             complement(7553..9100)
                     /locus_tag="PLG78_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNE22202.1"
                     /translation="MKACVFFVSLLLSLSGSLRAQAGTIPVILSSESTIYESVLSGIG
                     SAMNRRLTVQYLDAIIARYPDPAAYFQALEAQKPELVITIGSAASTLARDNLKQTPIV
                     FSMVNTPRLLGVGSRNICGVGMGISIGEFFRSIRDIAPTARFVHAFYTTDAGETEAME
                     GDYLDYQNKLVFVRHRLSGPAELGSALEKIKGHSDAILIVNDPLYSRTAFEKVSSFSS
                     QNHMILAAPFPALVKLGTTFGISPDYSKIGVQTGEMALRVLSGKSTCYAELVQPPEHN
                     AFYLNEDYAAKSGIAIPPKIVARAKLTQQFALGVDLLNQGKLKSARLVFENILKADPS
                     NNAASSFLQVALERQSGTRVTELLAQADRYMAEKQFALAVSEYRRVLSINPNVPQARL
                     GLDQSLQGQSDQERERAQALEAQGRPFDAIRTYMQALQTLPGNQNAIQGLAALRARQA
                     PQMQELVRHGVQAYDARDYGEAISIFDNVLLVIPGQKEATEYLRLAQKKRAALNILQT
                     KDAEVRQ"
     gene            complement(9097..9597)
                     /locus_tag="PLG78_03500"
     CDS             complement(9097..9597)
                     /locus_tag="PLG78_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22203.1"
                     /translation="MARALFMVTRALEADADNSQAMALEARIREAQSADPTLSGEGET
                     SARMAEAEELYAKKKYKQAIDLFLDVARMDARQNRALFRAANCYVFLNRLESAAHYYR
                     KSLAVHAEDVHVLNNLGSVYFRLKKYDEARESWNEAVRLQPGFQPARVNLERLDRFTS
                     QMETPV"
     gene            complement(9700..>11522)
                     /locus_tag="PLG78_03505"
     CDS             complement(9700..>11522)
                     /locus_tag="PLG78_03505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22204.1"
                     /translation="LIVLYRNQAVVLDKTYEVCLSVTNHISGLATEELLVNETYDATQ
                     TAFARLRQSKVTGLEDAYIINIDGEYVAEYNNAGRIHQKARQETVQLMRSLASPKTET
                     TTINGKPTILFSFPILIRSKSQTLRVGAAFFEFNRDEVYAPVNQIRNTILVVAGILFV
                     FGIAMAYYVAYFFSRPIQKLSEGARLIGEGDLNHRIRLYGKDELGRLATTFNQMTQKI
                     QDFTHNLEAMVDQRTRELNETLAQVQALKIQQDGDYFLTAILVEPLQPNNNKCDNVRT
                     EFRIEQKKKFHFRKWNSMIGGDVCITDTITLMDREYAVFINGDAMGKSLQGAGGVLVL
                     GAVFNSSLVRTHLQKNLKIYPEGWLRERFMELQAVFTSFNGSMYISVCMGLVDNLTGM
                     LYYINAEHPFTVLYRKGKATFLEQELELRKLGMPEQEEQFRVRTMQLLAEDVLIAGSD
                     GRDDIVIRNPQTGEEKVNDDEFLFLKHVEQADGLLDGIIDRLKASGTVLDDISLLRLS
                     YRENNPAPIAHIPVEIRETIADAQRLVEAGRHEEVLARLEDLFAANRSLPELLKTLGA
                     IYFHKQEYARAAECFESYLEEVPSDNEYIYALSNVYRLGG"
BASE COUNT         2840 a         3013 c         2809 g         2860 t
ORIGIN      
        1 caaccacttt cgcgaacttt gaccatcatg attcttaagg aaataaactc cagaagcaac
       61 ccagagaaat gccagaagaa aaaccagaaa tgaaccgagc ggctcattga aaacggtcgt
      121 gccgagcgtt acacaggggc gcaattcgca ccactgagac ggagtgaccg gcggctgttg
      181 ctgaagctcc gcgagccggc acgaagctac aagcggaaga gttagaacca gaaatgctgc
      241 attcagaaac agtcgcatat ctcgtaattg gacgaacgcc ctggaatctg caacgacttt
      301 cgatgaagct gcagcatagc ggcgaacaaa ctcatcgaat ctgaaagtcg tggatcctta
      361 gatcaatgag gacaggcccg cccataggct ttcctccaaa tccctgcgga gtcacaatac
      421 gatgagatgt aaacagtgct ccgtctccct ggctgtgctc tttgactacg ttagctgcgt
      481 ttgcaaactt gctgatgatg gcaggggcat agttgtgctg gagtagaaga cctgactgaa
      541 catcaaactc aaaggtctgc accctgttgt gagttggaat ggaatccggg aagatgccct
      601 cgaggaatcc gggacgcttt tctgtccagg tgatcgcatc gttcatgagc agtgcaggca
      661 gagtgagata gttccaggtg gcgtagtttg caaaatacgt catgtccaga tcgtcccagt
      721 aaaaaagacg cctgccgaat ggaaagtagc tgcgcgcatt cttgcgctct tccaggatct
      781 ctccgctgga attttcaaga cgcacatctg ctccgtcgag cactccagtt acgccggtct
      841 cctttccgat aggtgtcatt ctagcaaacg ggcgatggat ttcagtcgta aatttagcac
      901 gctcaaaagc gggccttcgc ttcaaagtga aagcaagacc gtttgcactt aagatcgttt
      961 cgattgtgcg cgccttattc cagagagccg tgcccccgta tgcctgaatt gctttttctg
     1021 cagtaacact caatttcata aacggaacct tcccggaatt cgtgctccaa ctcagcacca
     1081 cggtgagtca aatccaccct ttttgcgcag ttttttattt cctgatttcg ggtctcacat
     1141 cgctttgagg ttcacaggag taccagatga tcacggatca acgcttccgt cagcttgcac
     1201 tgtcgtttca agacactaca gaagtgcccc acttcgatcg cgctgcgttt cgcactccaa
     1261 agaggatctt cgcaaccatg ggcaaagacg gcgtcaatct gatgctcgac acggaaaccc
     1321 aggcaattgc agttaagaaa caccctcgcg ctttttctgt tctacagaac gcctggggta
     1381 agaagggcgc cactcacgtg gatctgcgcg cagtatctga agcggctctc gtgcccatcc
     1441 tcggcattgc ttacaaaaga gcaaccgttg gaatgcgaag cgcagctacc cgccgcaaac
     1501 tgcgagaatc aaagacgaaa gcataatcaa cggccgcttg gctccgctgc atctgattcc
     1561 ttcaaccgcc tgaaaagttc cggcagtttt ggaaacagtg cgtggattct gtgcatgacg
     1621 atggcaggaa gagcaggaat accaaaataa atggttccct cctgcgaatc tgctgtgagt
     1681 gcagacatac ccagtagaac agatcccttt cgcattctta gatgttctgc aatcgcagac
     1741 tgccctccta tgatcaccct gtcttccaga accactgaac cggcaagaac cgtcgcccct
     1801 gcaataaaga cgtaatttcc aactctgcaa ttgtggccga tgtgcacatg atcgtcgaac
     1861 ttcgtgtagt ctccgacgag agtgctttcg atcgtagcgc gatccacggt gcagcaagct
     1921 cccatttcta catgatttcc aatgattacg tttccaatct gaggaatctt gtgtcgcact
     1981 ccgcctttat cgtagaagcc aaatccgtct gcgccaatga cggtgttcgc gtgaatgagt
     2041 gcgtttgttc ccaggctgca gttgtatcca atcaccactc cggccatcaa aaccgactga
     2101 gcaccaactc tggcgcgttc ttctacaaca acgtttggat agagaacaca accgtctccg
     2161 atcacgcaat ccttcatgac tacaacgttt tccataatcg atacgtcttt gccaatcttt
     2221 gcggttggat gaatggacgc gcgcggagaa attccctttt ctggctggct ctttttttcg
     2281 aagatgccca gaagctcgat gaacttcaga cgcgccattt ctggaggatc tacaacaatc
     2341 gcatactcga agttatgcgc tagctccggc acagtgagag caacttttac acccgcaatt
     2401 ttctccgaat atttcttcag cctctttttt gactcaacaa agtaaatgtg atctggagat
     2461 gcttcccccg ggctttccag gtctttgatt cctgaaattt ccttgtccgc cttcccggcg
     2521 catttcatgc ccagcttttg agacaattcc tgtagagtca taggtcctcc gacatcgtga
     2581 cgcgcacacc gtattgaaca gcgttaaaat caaatggatt tgtccaatcc atgcgcagtc
     2641 ttgtttcgga cccgtgcccg gaaacgatca aaacgtcaag gcagaacgga cggatgtttc
     2701 ctaattctcc gcacgcttgt tttggttgca atcgccggca aatcggcaca ggatgcatcc
     2761 atcgtgaacg aagaccggca ccagcgcaga aaaagattca gaagcaacaa tatcccaagg
     2821 ctgctgatcg ggactcgggg cgctgatatg gttccatcag atcggaatgc acgagattgt
     2881 ttttcggatt actacaatca tcgtggccta cattgttttg tggacaacgc tgatcgagct
     2941 aaacattgga attatttata aagaaaattt acgatacctg cgcctcatca ttgattcctg
     3001 cggcctgacc attggaatcc tcatcacggg aggggccagt tctccgggca tcgtgacata
     3061 cgcagggaca acgggtcttg cgtcgctcta catttcaaag cgattcggtg tcttttccat
     3121 agcaaccaat gtcacgctct ttaacgccat gctgtttctg gttcacgagg gcatactgcc
     3181 acgcatcaac gttctggccc cggatgggct gggatcggaa tctacgatgg cggaaatcat
     3241 acttgcgaat atcatgatga ttctgtattc catcatactt cacctggtca tccagcccaa
     3301 ttatcgcaat ctacacacgg aacgcgcgga actgaaagcg cgaaacgacg ccatgcacag
     3361 agagattact ctggcccgcc gcattcaaga gaaactgatt ccggcgcgaa gtccagacga
     3421 gtctatcgcc tttctctaca agccaatgga ccaggtgggc ggagatttct ttgatttcat
     3481 agaacacgat gggaagatcg gaatttttct gtgcgatgtc tccggccacg gggttccagc
     3541 tgcactgatc gcatcgatga tgaagaccat cgtccttcag gctggacacc gagcgtccga
     3601 tcccgcaaca ttcatgtcct acatcaacga gactctccag agtcgaaccg cggggaattt
     3661 cgtgactgcc ttttatgcga gcttccagaa atcgagcaga agcattgtgt actgcaacgc
     3721 gggacattgt tcgccttacg tcatagatca gacaggcgta cgcgaactgg agggagtaaa
     3781 aagcgtcccg ctgggactct ggtccaacga cgagcttcgt ctgcgaaaca gagctttctc
     3841 aaatgttgag atcaacctga tcgctgattc gagaatactc ttttacaccg atggactcac
     3901 agaaacacgt tgcgagaacg gagatctgat gtttgaggag ggtccactcc aggaaaacct
     3961 ggtggcaacc aggcaacaga cttctgcggc cgcagtagaa gacctccacc gcgcgttggc
     4021 ccggtttcat ggtagcaact cattcgaaga cgacgtttgc gtgatctgcc tggatattaa
     4081 gtgaagcgag cgacatcact gcttcaagtc ccacgttcct acttctttca aggagcacaa
     4141 caacggcccc ggccaacggc tatccggcga actggcagta cgcgcagtaa tttgcggctg
     4201 gtctattgcg agggccacaa gataaatcac agacgcacag tgctccacgt cgcgacgcca
     4261 gtaattcgaa ccaggcttga tctgtgcaag atctgcgata aagaaatcca ggccaagact
     4321 cgtcgcgttc tgatctgaga tgtctttaac aaaaaaccct gccagacgat cctgaaggat
     4381 agatcgagct tcatcgccgg aaacagtgga agggttcaat cccacatctg caaaaactcc
     4441 gcagccagag agaaacaaca caaagacgct cgtgacaagc atgcgaaacg cgctcaaaat
     4501 ctgtaacctg ccttcaggaa gaattctctt ccgaatccgc gcaactggtg cggtgcaaag
     4561 ttaggactgc tgaagctttc gctatcaaaa tcgagtgggt atagaaccac tcgattgagt
     4621 atgttcttaa ttgagaacac gaagaacaac tcgctgtcca ggtttgaaga aatcgtcaag
     4681 ttgtagaacc acgatggcgg aaccttgtag atccgatagt tgcgaatatt tcctttcccg
     4741 gacagggagt ttcgatccaa ttctgcaggc agaggaggca aagcgttcaa ctcaagattg
     4801 ctatccagaa catcctgcaa agtggcagga ggtctctcca ttaagtagat ggggccgttg
     4861 taatagtact caatggcaac ggttgccttg ttcccgaaaa atccgcgcga tacgttcgct
     4921 ttatacaggc gagtcggaga gctgagcgct tgatcggatg gagtatcaat cttatccaga
     4981 aaactcacat cactggcccg acgcagtctg tagctggttc cacttccccc aaatttccag
     5041 agatcatccg gcgaaacaga aatggccacg gagcctccgg tgttagacca tgcaggagat
     5101 tgtccgaaag tagctgtggc ggcattaaaa gcgtataacg tcgaaccgcg aagccagaag
     5161 tgatcgagcg atacactcac tcgaccggag aacaaatcct ggatcaaatt cacttcgcga
     5221 ttgatcactt tttcggactc cgcatttccc tggtcgctcg ggagacgaat gagctcctgc
     5281 ggcgtcaccg cgcgaaatgc ttctgaatag atcaacttca gagttgtcgc agtctgctca
     5341 atcgattgaa tcagggcaaa gcgaggggtc gaatcctgtg caacgacatt cttgcgctga
     5401 aatggttggc gatagccctg gagcatgcga acgtactggg ccactctcac ctgaccggtg
     5461 accggatcaa agaaaggaat gaaatccgca tttgtgtccg tcggcacgct ggtctttgtg
     5521 attggattgc tgatattttc tgcggccgtg taagcagcat ttccatactc atgtctgtac
     5581 acacggttca ctttctggta gtcgtaacgc atgccggcga tggctgacgt gccgcttgcc
     5641 agaccaagcg ttagctgacc gaatgctccg tattgcctac cgtcatcacc gattccctgg
     5701 ttgtagagat agacttcatt tctttgattt tcaattcgct gatagttgat ataatttact
     5761 tccgcgccgt tgccaccgac aagtgcatgg atctttctca cgaaattctg gctggattcc
     5821 attcgaaacg gcgtgaattg aaattccatt ccatttgcgt aagcggtgga agcatacgcc
     5881 ttgatcatac tgcctccgtt cagcgccatg aactgtgtgt attgcgatga tgcaaggcga
     5941 gcggtagcag aagtgccgca gcaggcggcg gtatttccgg caaatccctc gaatcccttc
     6001 tcacgaaaat ccgaattgat gccaagctgg cgaaacacgc gaatggaagg ttcgtatttt
     6061 tccagatagg aaggacgata ttccagcatg gcgtaaccaa ctccccagat tctatcgttg
     6121 atggcggatc cgaactctgt ctggctacca aagacgcctc cgtctgttct tggatcgacc
     6181 cagcttacgc gtttagaaag atagagggct ttgacggaaa aatctttgta agtgatatca
     6241 aaattgaagt tgggaaatgg agaaccattg tccacttgag aacccgtggc caaccagatc
     6301 ctctgtcccg tgtagtaagg catatcttct ctgcctgacg ctgaagtctg cgagctagaa
     6361 cccagtcgat agtcgtagta tcgcgtgctc ccccagctcg gttggccgct gcgataaacg
     6421 ccagttcctt cgaaatgaga aacgtctgcc gtcacgctca gatcatttga aaacttcgac
     6481 aggtaaagag cgcgcacggc gaaagtccct gttccggcat cgtagtccgt ggatgtttcg
     6541 atttcatttt ctcgcgtagg ctttcgcgtc acgatattga caacgccggt gatggagttg
     6601 tttccgtaga gcgcggcacc agggcccttg atcacttcaa tgcgatcaat gcgatccata
     6661 tccgtataga gatccgaata aaactcaccg aagtgaaagt agtcgttcaa cttctttcca
     6721 tcctgcaaat acagcacacg cctttcgatc tggttgttga tgaacagacc gcggaaatca
     6781 gcggaccagt tctggcccac atagtgactg taaacctgcg gaatcgaatt tagagcttca
     6841 ttcagattcc ggtatccaaa acgacgaatt tgagagcgat tgattacctg aacgtccgcc
     6901 ggtgcttcag aagccttttg tgcgaatttc gaaacggcga ccacaatctg atctcgttcg
     6961 gaaattactt tgaacgcttg atcggcctga gactgaacat tttcctgagc caggcgaaat
     7021 tgaatctctt ttccaatccg ctgactgaga atcgcttcat tgatgctatc tcttctttcc
     7081 gttcgacgcg ggtttgaaag gatgcgattt gcaaggcgaa ttgagaaatc ttcaattctc
     7141 ctgggagtcg gagtacgcgt ctcgtcctct ggaagcacga gcccttcaat ctgacgcgat
     7201 tcatccaccg cattgagcgc gtcgatcaca actcctgtct ctggattgta gatctggcca
     7261 tagatgttga cggagccacc actcacgttg taataaccgt gaattacaaa ggcagctttt
     7321 cgctgacgcg cctgcgcaag agcatcattc aaaacagcac tcggagcttc ttccactaca
     7381 actccagatc cagtgagctt gccggaaaca gctgtgcgca ggtcgcgtgc gagtccggga
     7441 tttggtgggc aatcaaacgg ttggaattca acaaccacga cgactgcatc ttctgcgttc
     7501 aaactcgaaa tcgagacaaa ggtgaatatc agcaggaaag aaaggaacga cttcattgcc
     7561 gcacctcggc gtctttagtc tgcaaaatat tgagtgctgc tctcttcttc tgcgcaagtc
     7621 gcagatactc tgtagcttct ttctggccgg gaattacaag cagtacgtta tcaaatattg
     7681 aaatcgcttc cccgtagtcg cgagcgtcat atgcttgcac gccgtgtcgt acaagttcct
     7741 gcatttgcgg ggcctgcctg gctctcagcg ccgctagacc ctgaatcgca ttctgattcc
     7801 cgggaagagt ctgaagtgcc tgcatgtagg ttcgaattgc atcgaacggt cttccctgcg
     7861 cctcaagagc ctgtgccctc tctcgctcct gatcactctg cccttgcaga gattgatcca
     7921 atccgagacg tgcctgaggg acattcggat tgatggacaa cacgcgcctg tattcgctca
     7981 ccgccagagc aaattgcttt tctgccatgt acctgtccgc ctgagcaagt agctctgtga
     8041 cacgcgtgcc actctgtctt tccagtgcta cttgcagaaa tgaagaggct gcattgttcg
     8101 atggatctgc cttgagtatg ttttcaaaaa ccagtcgagc agacttcagc tttccctggt
     8161 tcagtagatc cacgcccaga gcgaattgtt gcgttagttt agctcttgcc acaatcttgg
     8221 gcggaatcgc aattccagat ttcgcggcat aatcctcatt caagtaaaac gcattgtgct
     8281 ctggaggttg caccagctcc gcataacagg tggacttccc agacaaaaca cgcagagcca
     8341 tctctccggt ctgcactccg atcttgctgt agtccgggct gattccaaag gtagttccga
     8401 gtttcacaag ggcaggaaac ggtgcggcga gaatcatatg attctggctg gagaagctgg
     8461 atactttttc aaacgctgtg cgcgaataca aaggatcatt cacaatcaaa atggcgtccg
     8521 aatgcccttt gattttctca agcgcgcttc caagctctgc cgggcccgac aatctatggc
     8581 gaacaaacac cagtttattc tgatagtcca ggtagtcccc ttccatcgcc tctgtttcac
     8641 cggcatccgt ggtgtagaag gcgtgaacaa atcgtgccgt gggagcgata tccctgatgg
     8701 agcgaaagaa ctcgccgatg gaaatgccca tccccactcc gcaaatgttg cgcgacccga
     8761 cgccaagcag cctcggcgta ttgaccatag aaaatacaat gggcgtctgc tttagattgt
     8821 cgcgcgcaag agtactggcg gcagaaccaa tggtaattac aagctctggt ttttgcgctt
     8881 cgagcgcctg gaagtaagcg gcgggatctg gatatctggc gatgattgcg tccaaatatt
     8941 gcaccgttag gcgacgattc atcgcggaac caatcccaga aagcacgctt tcataaatcg
     9001 tgctttctga tgacagaatt acaggaatgg tacccgcttg agctcgtaga gagccagaaa
     9061 gactcaacag aagacttaca aaaaagacac atgctttcat acgggtgtct ccatctgtga
     9121 agtgaaacgg tccagccgct ccaggtttac gcgtgctggt tgaaatcctg gctgcaaacg
     9181 cactgcctca ttccatgatt cacgtgcctc atcatatttt ttgagtctaa aatacacaga
     9241 accgagatta ttcaaaacgt ggacgtcctc tgcatggact gccaggctct tccgatagta
     9301 gtgtgccgcg ctctccaaac gattaagaaa cacatagcaa tttgctgcac gaaagagtgc
     9361 gcgattttgt ctcgcatcca tacgcgcgac atccagaaat aaatcaatgg cctgcttgta
     9421 tttcttctta gcgtagagtt cttctgcctc tgccatgcgg gccgatgttt ctccttcgcc
     9481 tgacagagtg gggtccgcag attgtgcctc gcgaatcctg gcctcgagtg ccattgcctg
     9541 cgagttgtct gcgtccgctt cgagtgctcg cgtgaccata aagagcgcgc gagccatcac
     9601 tcccatttcg aaatagacgg cgctcagatt caggagattg agtaggtgtt ttctgtcgcg
     9661 aagaaagaga cgttcaccga ggtccgcgga gcgattcagt tacccgccca gtctgtacac
     9721 gtttgagagt gcatagatgt attcgttgtc cgaaggaact tcttcgagat aactttcaaa
     9781 gcattcagcg gctctcgcgt actcttgttt gtgaaaatag attgcgccca gagttttcaa
     9841 gagctcgggc aaagatcgat tggctgcaaa gagatcttcg agacgcgcca gtacttcctc
     9901 atgccttccg gcttccacca atctctgcgc atctgcgatt gtttctctga tttcgacagg
     9961 aatgtgagca atcggggcgg gattgttttc gcggtaagaa agcctgagaa gagaaatatc
    10021 gtccagaacg gtgccgcttg ctttgagcct gtcaataatc ccatcgagca aaccatctgc
    10081 ctgttctacg tgcttgagaa agagaaattc atcatcgttt actttctctt cgcctgtctg
    10141 tgggtttcga atgacgatgt catcacgtcc gtccgatccg gcgatcaata cgtcttctgc
    10201 cagaagttgc atggttcgaa cgcgaaactg ttcttcctgt tctggcatac ccagctttcg
    10261 cagttcgagc tcctgctcga gaaacgttgc cttccctttg cgatacagta ccgtgaaagg
    10321 atgctctgcg ttgatgtagt agagcattcc ggtcaaattg tctacaagac ccatgcacac
    10381 ggatatgtac atgctaccgt taaacgacgt gaatacggcc tgtagttcca tgaaccgctc
    10441 acgaagccag ccttcaggat agatcttgag gtttttctgg agatgagtgc gaaccaggct
    10501 tgagttgaat accgccccca aaaccagaac gccgccggct ccctgcagag actttcccat
    10561 cgcgtctccg ttgatgaaaa cggcgtattc gcggtccata agcgtgatgg tatccgtgat
    10621 gcagacatca cctccaatca tggagttcca tttgcgaaaa tggaatttct tcttttgctc
    10681 gatgcgaaac tccgtacgga cgttgtcaca cttattgtta ttgggttgca acggctccac
    10741 gagaattgca gtcaggaaat aatccccgtc ctgctgaatc ttcaaagcct gcacttgagc
    10801 cagagtttcg ttgagttcac gcgtgcgctg gtctaccatt gcctccaggt tgtgagtaaa
    10861 atcctgaatc ttttgggtca tctggttgaa cgtggttgcc aggcgaccca gctcatcctt
    10921 cccatacagg cgaatccgat gattcagatc cccctcccca atcaatcgcg cgccctctga
    10981 gagcttctga atgggccgac tgaagaagta ggcgacgtag taagccatag caatgccaaa
    11041 cacaaagaga atgccggcca cgaccagaat cgtgttacga atctggttta ccggtgcgta
    11101 cacttcatcg cgattgaatt cgaaaaacgc agccccaacg cgcagtgtct gactcttgct
    11161 tcgaatcaga atggggaagc tgaaaagaat cgtgggcttc ccgttgatgg tggttgtttc
    11221 tgtctttggc gaagcgagac ttcgcatgag ttgcaccgtc tcctgcctgg ctttttgatg
    11281 aattcttccg gcgttgttgt attccgctac gtactctccg tcaatgttga tgatataagc
    11341 gtcttcgaga cctgttactt tcgattgcct gagcctcgca aatgctgttt gcgttgcatc
    11401 ataagtttca tttacaagaa gctcttcggt tgcgagacct gatatgtgat tggttacgct
    11461 gagacagacc tcatacgtct tgtccaggac caccgcctgg ttgcgataga gcacaatcag
    11521 tg
//
LOCUS       DAOQJL010000048        11511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2830_length_11511_cov_4.225995, whole genome shotgun sequence.
ACCESSION   DAOQJL010000048 DAOQJL010000000
VERSION     DAOQJL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_11511_cov_4.225995"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLG78_03510"
     CDS             <1..513
                     /locus_tag="PLG78_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22205.1"
                     /translation="ARSYLTTVIPREIVGAVSSRGFLVHTREDDNGQKSLREALLTES
                     SIKGAPIKVIVPVTEQYVLAYRMAVVQRQQVETERAVIGALSCLSLLALQFWSVSTHE
                     EIMNLDIVMMDVRLSRIAWRRHYSENLSQLKFLDNQYFGFAPFTRDRLSHFPTALKLQ
                     TAVVPSSEGK"
     gene            603..1100
                     /locus_tag="PLG78_03515"
     CDS             603..1100
                     /locus_tag="PLG78_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNE22206.1"
                     /translation="MLFKAKSEVAIIMDYQEENRASPESDIIRKRIASLVQAQLIERG
                     FRVLDQSPDVGENFDWRRKDHRDALFPAYGKQPVLLFVVKVTALKTQVESSLPLTCVR
                     FALLDYRDPDSFVEYTVSADVQSVVFSTGEVLHERHIKRPLHDYTQDSIVDTAGWLTI
                     WSEVR"
     gene            1150..1668
                     /locus_tag="PLG78_03520"
     CDS             1150..1668
                     /locus_tag="PLG78_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22207.1"
                     /translation="MALRCILPFFFLLFGCSLETAAPYILKSSAHEWASQAADGHGFL
                     ILSLSERSNCKFEYLSIEAKDDLGTVLRNPDPSYFNTAAPRGQNPERFLCGYDYALPI
                     RAGNWEQIRIFRRSIISDVTARNHLQVQPNTAYIFALSEDSAHPWSESELSVEEYRAR
                     ITRSGLRVVGLK"
     gene            complement(1698..2066)
                     /locus_tag="PLG78_03525"
     CDS             complement(1698..2066)
                     /locus_tag="PLG78_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE22208.1"
                     /translation="MKILTVDDSSTVRFVMKSAIESLGHTCLMATNGQEALAIVSAEP
                     IDVVLLDWNMPLMDGREVLREIRKKPICKNTKVLMCTSEATRGDVEQILRLGVEGYIL
                     KPISPDKLKAELAKIGVGPA"
     gene            complement(2103..3923)
                     /gene="uvrC"
                     /locus_tag="PLG78_03530"
     CDS             complement(2103..3923)
                     /gene="uvrC"
                     /locus_tag="PLG78_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNE22209.1"
                     /translation="METGAEIVQAVQRAPTAPGCYIWKDSSGEILYVGKAISLRARLR
                     NYLNPQDTKTQFLMDRAATLEWVTTETNREALILEATLVKKHQPRFNVRLKDDKRYPW
                     ICISMSEMYPQIFITRTVRDDGNLYFGPYTDVGATRNTLSLILKIFPIRKVRQKLPLP
                     KPKRPCMNFHIGRCLGPCQGNVDPEEYARIVEQVRLFLEGRTEILETLVGKRMEEASE
                     RMDYEKAAMFRDVLANIRRATERQIISDPGAADEDIVAFAARDDDGQMVVFEMREGKL
                     TGRKSFPLKGVLNSDPEEIMTSFVRDCYLNASIRPATIILPGKIKAKRELEEMLSENG
                     RSIRITTARKEEAELLSRMAQKNAELLLRERLLAARMKDRSRGLEEIKEMFSLPEVPS
                     VMECYDISHLQGSHTVASGVFFVDGEPSRKDYRHYKIKTVPGIDDPASMEEVISRRLQ
                     RLLAEDRALPDLILIDGGTTQLAAACRAANALGAGDVPIIGIAKQREEIYFPGEPTPR
                     HFDKDSAGMLILRHMRDEAHRFGITFHRSRRNQAVMRHMVEEIPDIGVSRLRSLLKHF
                     AEHSIEEATVEELMKVPGIGKALAGKIHGYFQNKTGAKTE"
     gene            complement(4014..4353)
                     /gene="rnpB"
                     /locus_tag="PLG78_03535"
     ncRNA           complement(4014..4353)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG78_03535"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4387..6213)
                     /locus_tag="PLG78_03540"
     CDS             complement(4387..6213)
                     /locus_tag="PLG78_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22210.1"
                     /translation="MKDTSAGLKLDSGGRNYFKQRKVEFRTIVLGAETLHGKWLRYDT
                     NLEPLDWSTHPEIPEIRAFALQASKRFLDFWNEVFDVAVARALATRDRGILRHKLFEP
                     SSYFDIGVPEEQRHSSIPPALHSKIISEYNASRFAETENHVYYLTEYLSLLSDDWIEK
                     LKPFEKLWNLTDEKARLTQEISELRATTEEEQNLLQAANSLFFHASRAHRIRLRLILL
                     RGQKRSASQLLDALSRSQTEIDSRYAILNEKPIESKDTFAGSGLSADSYLSLCRSILD
                     TNSAIESIYLKDVDPALERSMVREACRDILHRLGPIRRAAAGYSLIAGERHGAMSLNP
                     VMRLWKEKPNPPRSEAPALSLEMNTKQTIDCLIRLAHLADPDLGHRELMRGKIAKLMR
                     GIENLANLKNFLIPGSAYPLREIHPLDYPEFRWRVIGETRSPLELGANETAVLTGAWY
                     SKRQHSLYCTIGADNGRLLRILWNSERSPGPPAFYFAMGQFVHDCMEDSLIYRRSGSI
                     TFRECVEDYYDFEDRIRKNKGEKTGRRKSDNSRPGVRFMFAVLYSRMITELLTGSLQS
                     QFRHPATEKWMETNLGLSRDRARLKHIRIKLREIISNLRVHA"
     gene            complement(6240..7775)
                     /locus_tag="PLG78_03545"
     CDS             complement(6240..7775)
                     /locus_tag="PLG78_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22211.1"
                     /translation="MPRFEPFPFTADIADNFRAKKQIPAHLYSKEGQILIYRKDGATD
                     GEIDKILKFAERGIYYNVDDAESLGLKGDRRDVPAGLSDTKLFASEKAQDLTRETQEI
                     FSQLRETAITSDTARKTKATMTQVFDDFESQPDAMIGLVNIIECMKDAVGAYDVQMAV
                     KRTVVSMAMKTRGMHANSFRERQKMQEMATVVMTSALFCDIGTTRMKMPTDVGLTPEQ
                     MSYVRNHPLMSYLLIAHQDEIDSAVKRNILCHHRPLRQGLPGNNYPELKPLILKLGQL
                     LENYEKEPSRQSIVRDLKQQIHSLKQDIPYDEDANILAIASEFASLTSRTSWREAHTP
                     QRAVRSIINNSYFTYAERAIREFLDHVAISLCDNKKIISEGDFIVLVNRSAEGKTFFE
                     VCQITSSSRYQSRPGVDRVAEILPIVDQSPKLKLTGFDISSIRPDKRFAHYELAQDDS
                     RRLVYAVDPELDTELYEKLSSLIQGRKRYEPEPKKDHKEKEKPAEAGAETHSAAHDKH
                     PKG"
     gene            7892..9946
                     /locus_tag="PLG78_03550"
     CDS             7892..9946
                     /locus_tag="PLG78_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22212.1"
                     /translation="MGVFLRLVKYSFRYRGRFLAGLGVAFLVAVLSAVSLNSFLPFFE
                     ALGDKSAEFALPSSRAERNLMKKAILASLKDKPLHVLILIEALKYPSEDKAWTDFLGL
                     TQPRGKEGKLDKVKSRILLALLPDKPTGVTDIESFQLRQIIYWKLRVNAYGFRPLEVI
                     YGVLMILVPLIALRLVLQIVTVRLIAKAGYRAVRDLRSDLYRKVQELPLTFFYSQKSG
                     VILSRMINDAEVVAAVISSNLRDAITNVFILVTHFAVLAYLNLQLLLVSLIAVPLMLS
                     PVTLFARKVRKSTGKSQEYLADLNALLKETISGVKVIRSFAMEGYEAEQFRRANQKLY
                     WRTFKQQFYLRASPNLVELTSAVVALGMLGLGAFFLDPTDFTGGQFMAFLVILLSLIR
                     PVIQLSGMMAKVGQGSEAGRRIFEILDRPRDIEEARHPAVLRPLRKHLEFKNVSFQYP
                     ETDKLVLENINLHVEAGQTIALVGESGSGKSTLMDLLARFFDPTSGSIRIDGTDIRDF
                     RIADHRSRIGIVQQENFLFHGPVRENIAYGRPEISIREIMKAARLAHAHDFIKELPGK
                     YESMIGERGLNLSGGQRQRIAIARALLRDPEILILDEATSALDTQSERLVQHALDRLF
                     RNRTVFVIAHRLSTIEKADLIVVLSGGQIVDMGKHSDLMQKGGLYARLQEISRQAVPG
                     LA"
     gene            9969..10388
                     /locus_tag="PLG78_03555"
     CDS             9969..10388
                     /locus_tag="PLG78_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE22213.1"
                     /translation="MKPVLLVDDNDRYAEMLDKYFRDRGYTPERAFNGKDALGMFKGK
                     PADYYRAIVTDITMESQLAGLSFLWEIKKLGFHGTIVVASTGFDFPLAMPLSRMVLTG
                     WGVHYLVPKAPMKRAGIIEFYPVSLFSSTLKEFQEVQ"
     gene            10427..11503
                     /locus_tag="PLG78_03560"
     CDS             10427..11503
                     /locus_tag="PLG78_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22214.1"
                     /translation="MTNFRRVLIISSLASLLTCVPGIPVKIPRYPEGVMGDQLKSVMG
                     AKRKVGVVAASPNPNLLQQIGYDQDWSATVEGAVTTELTQRGFYTMIDTSSRKQRLRE
                     LAYTQTGLTGEQRNIGQELAAEGLLFIRMTAPPLKECKTEYVTDFAATALKLGMKMAM
                     KDSNGAGDAQEKKPTGVLFLTVFVQGTLTNLETGQSVTYAHSKPYKLQNEAGNQECPS
                     TLDAFGKALQDASAAIADNLSPSIVTVKIPLASDADDLDGDQKAKVTKYLTEGIKWAE
                     ADDFQMAAQNWEKALDASGGKSASALWNIAAYKWYSGDMDGAEKYFKRSFSNGGSDWA
                     DAAKRSLWATFRKEKDRKESEGGN"
BASE COUNT         2636 a         2889 c         2964 g         3022 t
ORIGIN      
        1 gcgcgttcct atctcactac tgtgattccc cgggaaatcg taggtgcagt ttcgagccgc
       61 ggattccttg ttcatacgcg tgaagatgat aacggacaga aaagtcttcg cgaagccctt
      121 ttgactgaaa gttcaataaa aggcgcgccg atcaaagtga tagttcctgt gacagagcaa
      181 tacgtcctgg cctatcgcat ggcggtggtg caaagacaac aggtggaaac ggaacgtgcc
      241 gtcattggtg ccctctcttg cctttctttg ttggccctgc aattctggtc tgtttctacg
      301 catgaggaaa tcatgaatct agacattgta atgatggacg tccgtctctc caggattgcc
      361 tggcgtcgcc attattcaga gaacctctcg caactcaaat tcctggacaa ccagtacttt
      421 ggttttgcac ctttcacaag ggatcggttg agtcatttcc caacagcact caagctgcag
      481 acggcggtcg tgccttcatc cgaaggcaaa tgacgattcg ggaaagcatg ggaatcgtga
      541 gaatctgcgt ggttgcgttg ggagttttga tcggatgttc cgtcgatccg attcgcactg
      601 aaatgctttt taaagcgaaa tccgaagtgg ctataatcat ggattaccag gaggaaaacc
      661 gggcgagccc cgagtcggat ataattcgaa agaggattgc gtcgcttgtc caggcacaac
      721 tcatcgagcg cggatttcgc gttctcgatc agtcgcctga cgttggtgag aacttcgact
      781 ggcggcgcaa agaccatcga gacgcgcttt ttccggccta tggaaagcaa ccggtcttgc
      841 tatttgttgt caaagttacg gcattgaaga ctcaagttga gagcagtctt cccctgactt
      901 gtgtccgctt cgcgttgcta gactaccgtg atccagattc ctttgtcgag tacaccgtat
      961 ccgccgatgt tcagtcagtt gttttttcca caggggaggt tcttcatgag agacacatca
     1021 aaagaccact ccacgattat acgcaggatt caatcgtcga tacggccgga tggctgacta
     1081 tatggtcgga ggtccgatag ccagaggcgc gaaggctgaa atttattggg agtaagatgt
     1141 ggagttcata tggcattgag atgtatttta ccattctttt ttcttttgtt cgggtgcagc
     1201 ctggaaacgg cggcgccgta cattctaaaa agtagcgcac atgagtgggc gagtcaggca
     1261 gcggatggac acggctttct cattttgtcg ctttctgaac gctccaattg caagttcgaa
     1321 tatctttcca tcgaggcaaa ggatgacctc ggaaccgttc tgcgcaatcc agacccgagc
     1381 tacttcaata ctgctgcgcc tcgcggtcaa aacccggagc gatttttgtg cggatatgat
     1441 tacgcgctcc cgatccgcgc cggcaactgg gagcaaatcc ggatctttcg ccgcagcatt
     1501 atctcggatg tgacagcgcg caatcacttg caagtacaac ccaacactgc ttacattttc
     1561 gctctgtcag aggacagcgc gcacccgtgg tctgagtcag agctaagcgt tgaggagtac
     1621 cgagcgcgca tcactagatc cggcctacgg gtagtaggcc ttaagtaaat cagaattcat
     1681 tctcgaattc cgcacaatca ggccggtccg actccgatct tcgccagttc cgctttgagt
     1741 ttgtccggag aaatgggctt aagtatgtag ccttctactc ccaggcgaag gatctgctct
     1801 acatctcctc gagtcgcttc tgacgtgcac atgagaacct ttgtgttctt gcagattggc
     1861 ttctttctaa tttcgcgcag tacttcgcgg ccgtccatga gaggcatatt ccaatccagg
     1921 agcactacat cgattggctc agcggaaacg atggcgagtg cttcctgacc attcgtggcc
     1981 atgaggcaag tgtggcccag ggattcgatg gcagacttca ttacaaagcg cactgtggat
     2041 gaatcatcta cggtgagtat cttcatgcta agattagacc tccgtgcttt gttttcgcaa
     2101 gcttattctg ttttcgcgcc tgttttgttc tgaaagtatc cgtggatttt acctgcgagt
     2161 gctttaccga ttccgggcac cttcatcaac tcctccacag ttgcttcttc gatcgaatgt
     2221 tcggcgaaat gcttcaggag cgagcgaagc ctggacactc ctatgtctgg aatttcttcc
     2281 accatgtgac gcatcacagc ctgattcctt ctggagcgat gaaatgtgat gccgaatctg
     2341 tgtgcttcat cgcgcatatg acgcaagatc agcatgcctg cactgtcttt gtcgaagtga
     2401 cgcggagtgg gttctccagg gaaatagatt tcctctcttt gctttgcaat tccgatgatc
     2461 ggaacatccc cggccccaag cgcgtttgcg gctctgcacg cggctgcgag ttgagtcgtt
     2521 ccaccgtcta tcaagataag atcaggaagt gccctgtctt ctgcaagcag tctctgcaat
     2581 cgcctggata tgacctcttc catggaagca ggatcgtcta tgcctggaac agttttgatt
     2641 ttgtaatgtc tgtagtcttt tcgagaaggt tcgccatcca caaaaaacac tccagaggcg
     2701 acagtatggc taccctgcag atgggatatg tcatagcatt ccatgacgga cggaacttcc
     2761 ggaagggaaa acatttcttt gatttcttcc aggccgcgag atcggtcttt catgcgcgct
     2821 gccagaagtc tttcgcgtaa cagtagttct gcgttctttt gcgccatgcg actgagcaac
     2881 tccgcttctt ctttgcgagc ggttgtgatt cgaatagacc ttccgttctc ggaaagcatt
     2941 tcctccagtt ctctctttgc tttgattttg ccgggcagta tgatcgtggc tggtcgaatc
     3001 gatgcgttca gatagcagtc gcgcacaaag ctggtcatga tctcctctgg atccgagttg
     3061 agcactccct tgagtggaaa tgattttcta cctgtgagct ttccttcgcg catttcaaat
     3121 acaaccattt gtccgtcgtc atcgcgcgcc gcaaacgcca caatgtcttc gtctgccgct
     3181 cccgggtcag aaatgatctg gcgttctgtg gctcgcctta tattagcaag aacatcgcga
     3241 aacatggctg ccttctcgta gtccattctt tcgcttgctt cttccattct cttgccaact
     3301 agcgtttcca gaatttcagt acgaccttct aaaaagagac gaacctgttc tacgatgcgc
     3361 gcatattctt ctggatccac atttccctgg cacggcccaa gacaccgacc aatgtgaaag
     3421 ttcatgcatg gtctttttgg ttttggaaga ggaagcttct gtcttacttt gcgaattggg
     3481 aagatcttta gaatcaaaga gagagtgttt cgagtcgcac ccacatctgt ataaggtcca
     3541 aagtacagat ttccatcgtc gcgcaccgtg cgcgtgatga atatctgcgg atacatttca
     3601 gacatggaga tgcagatcca tggatatcgc ttgtcatcct tcaggcgaac gttgaatcga
     3661 ggttgatgtt tctttaccag agtggcttcg aggatcagtg cttcgcggtt ggtctccgtc
     3721 gtgacccact cgagagtggc cgcgcgatcc atcaagaatt gcgttttggt atcctgtgga
     3781 ttcaggtagt tgcgaagtct tgcgcgaaga ctgattgctt tgccaacgta cagaatttcg
     3841 ccggaagagt ctttccagat ataacagcca ggagccgttg gcgcccgctg cactgcctgg
     3901 acgatttcag ctcccgtttc cactgagtca aaaaagcgat tcgcaggccc actgtcagca
     3961 tcattcagtg ctcgtgtcac acttcaagaa aagcggccga atcgcccgga aggtgaggga
     4021 gtgcacctgt aagccggatt ctgtcgtgga tcgccattta tcttgagaca agcttctttg
     4081 ctctctaccc gcagtcccgg tcggaccgac ctctgactgc ctatgcgaga ttgcaccgga
     4141 gagggtttgc cgtaccacaa atcttacgat ttgcgcggtg ggctcttacc ccgccatttc
     4201 acccttaccc cgaagggcgg tatctttctg ttgcactttc cgtcactgcc gttagacgac
     4261 aatgcccggg cgttacccgg tctcctgtcc tgtggtgtcc ggactttcct cactccctgc
     4321 aaagcaggtt cgcgcggcga tccggtgcac cccctcacag gtaggatgct aaatctgatt
     4381 tcaaagtcaa gcgtgaacgc gaagattcga aatgatttcc ctcaacttga ttcggatgtg
     4441 cttgagcctg gcccggtcac gactcagtcc cagatttgtc tccatccatt tctccgtggc
     4501 aggatgacga aactgactct gtagcgaacc ggtaagtagt tcagtgatca tgcgggagta
     4561 aagcacggca aacatgaatc gaacgccagg ccttgagttg tctgattttc tgcggccagt
     4621 tttctctccc ttgtttttgc ggattcgatc ttcaaagtca tagtagtctt ccacgcactc
     4681 tctaaacgta atgcttccgc ttcttctata aatcagagaa tcttccatac aatcgtgaac
     4741 gaattgtccc atggcgaaat aaaaagcagg tggtccagga gatctttccg aattccagag
     4801 gatgcgaagg agtctgccgt tgtccgctcc aattgtgcaa tagaggctgt gttgtctctt
     4861 gctgtaccat gcacctgtca gcactgcggt ttcatttgcg cccagctcca gagggcttcg
     4921 cgtttcacca atgactctcc atctgaactc agggtagtcc agaggatgga tttcacgcaa
     4981 agggtaggcc gatccaggaa tcaggaagtt tttcagattg gccagatttt caattccgcg
     5041 cataagcttt gcgatttttc cacgcatcaa ttctctgtgt cccaggtccg gatcggcgag
     5101 atgggccagc ctgatcagac aatcgatcgt ttgttttgta ttcatctcaa gcgaaagggc
     5161 aggggcttca cttcgaggag gatttggttt ctctttccac agacgcatca ctggattgag
     5221 gctcatggct ccgtgtcttt cgcctgcaat cagcgagtat ccagcggccg cgcgacgaat
     5281 cggccccagg cgatgaagga tatcccggca tgcttcgcgc accatgcttc gctccagggc
     5341 aggatccaca tctttcaagt agatggattc gatcgcagag ttcgtgtcga gaatcgatct
     5401 acacaaagaa agataggaat cggcggaaag tccggaaccc gcgaaggtgt cttttgactc
     5461 gatcggtttt tcgttcaaga tcgcataacg tgaatcgatt tcggtctgcg aacgagagag
     5521 tgcatcgagt agctgtgaag cggatcgttt ttgaccgcga agcaagatca gtctgagcct
     5581 gattctgtgg gcgcgggatg cgtgaaaaaa caaggaattc gcggcctgca aaaggttctg
     5641 ctcttcctct gttgtggccc gaagttccga aatctcctgg gtcagtcggg ccttctcatc
     5701 cgttagattc catagctttt cgaaaggttt gagcttctcg atccaatcat ctgaaagaag
     5761 gctcaggtac tctgtcaggt agtacacatg attttcagtt tctgcaaatc gcgatgcatt
     5821 gtattctgaa atgattttgg aatgaagcgc aggaggaatc gagctgtgac gctgctcttc
     5881 tggaacaccg atgtcaaaat aagaggacgg ttcaaaaagt ttgtgtctga gaattccgcg
     5941 atcccgcgtt gccagagccc gtgcgaccgc tacatcaaag acttcattcc agaaatccag
     6001 aaatcgctta gaagcttgca gagcgaatgc tcgaatctca ggaatttcag ggtgagtgga
     6061 ccagtccaga ggttccaggt ttgtgtcgta tctcagccat ttgccgtgaa gagtttctgc
     6121 acccagtacg atggtgcgaa attcaacctt tctctgtttg aagtaattcc tgccgccaga
     6181 gtcgagtttc agaccggcgg aggtatcctt catcaggaaa gaaatgatgt cttctgcttt
     6241 caacctttcg gatgcttgtc atgcgctgcc gaatgtgttt ccgcaccggc ttctgcgggc
     6301 ttctcttttt ccttgtgatc cttcttgggc tctggctcgt aacgtttgcg gccctggatc
     6361 aaggaagata atttttcata cagctctgtg tcgagttctg gatccaccgc atagacaaga
     6421 cgtctgctgt cgtcctgtgc gagttcatag tgtgcgaagc gtttgtccgg tcggatactt
     6481 gaaatgtcaa agccggtgag tttcaatttg ggcgattggt ccacaatggg aagaatctcc
     6541 gccacgcgat ccactccagg tctgctctgg tagcgcgaag aagaagtgat ctggcaaact
     6601 tcaaagaatg ttttgccttc cgcagatcga ttcacgagta caatgaaatc tccttcagag
     6661 atgatcttct tgttatcgca aagactgatg gcaacgtgat cgagaaattc gcggatggct
     6721 ctttccgcat atgtgaagta ggaattgttg atgatgcttc tcactgcgcg ttgtggtgtg
     6781 tgcgcctctc tccaggaagt gcgcgaagtt aaagaagcga actcagatgc gatggccagg
     6841 atgtttgcat cttcgtcgta tggaatgtcc tgcttgaggg aatgaatctg ctgcttgagg
     6901 tctcgcacaa tcgactgtcg cgaaggctct ttttcgtaat tctccagaag ctgaccgagt
     6961 ttcagaatga gaggttttag ttcgggatag ttgttacccg gaagcccctg gcgaagaggt
     7021 ctgtggtggc agagaatatt tctttttaca gcgctgtcta tttcatcctg gtgcgcgatc
     7081 aaaagatagg acatgagagg atgattgcga acatagctca tctgctctgg agtgagtccc
     7141 acgtccgttg gcattttcat gcgagtggtt ccgatgtcac agaaaagcgc actggtcatg
     7201 acgaccgttg ccatttcctg catcttttgt ctttcgcgga agctgttcgc gtgcatgccg
     7261 cgcgtcttca tggccatgct tactactgtt ctcttcaccg ccatctgcac gtcgtaggca
     7321 cccactgcgt ctttcatgca ttcaatgatg ttgaccagac caatcatcgc atctggttgc
     7381 gattcgaaat cgtcaaagac ctgcgtcatg gttgcttttg tttttcgggc agtgtctgat
     7441 gtgatggcgg tttcgcgaag ctgagagaag atttcctggg tttcccgcgt gagatcctgt
     7501 gccttttcgg atgcaaatag ttttgtatcg ctgagcccgg cgggaacatc gcgcctgtct
     7561 cctttgagac cgagggactc tgcgtcgtcc acgttgtagt agatgccgcg ctctgcgaat
     7621 ttgagaattt tgtctatttc gccgtcagtg gccccatcct ttctgtaaat aaggatctgc
     7681 ccttccttac tgtaaaggtg ggccgggatt tgttttttcg cgcgaaaatt gtccgcgatg
     7741 tctgccgtaa acggaaacgg ttcaaatcgt ggcacaagtc aaaaaaaaca aacgtgggca
     7801 ggccttcact taaaaaaaat gcgctctgcc agtctggatt tgtacaggct tgtttggatt
     7861 gccgggaaag ccgcgggcgg gataatagac tgtgggcgtc tttctccgcc tggtgaaata
     7921 ttcgtttcgt taccggggcc gcttccttgc tggccttggc gtcgcattcc tggtcgcagt
     7981 cctcagtgcg gtcagcctca attcctttct gccctttttc gaggcgctcg gggataaatc
     8041 cgccgagttc gcgctgcctt cgtctcgcgc agaacgcaat ctgatgaaga aggcgatcct
     8101 tgcctctctc aaagacaaac cgttgcacgt cttgatcctc atagaagcat tgaaatatcc
     8161 ctccgaggac aaggcatgga cagacttcct ggggttaacc cagcctcgcg gcaaggaagg
     8221 gaagctagac aaagtcaaat cgcgcatatt gctcgctctg cttccagaca agcccactgg
     8281 cgttacggac attgaatcgt ttcagctcag acagatcatc tactggaaac tcagggtcaa
     8341 tgcgtacgga tttcgcccgc tcgaagtgat ctacggcgta ctgatgattc tggtaccgct
     8401 cattgctctt cgtctcgtgc ttcagattgt aacggtgcga ttgattgcga aggcaggcta
     8461 cagagccgtt cgtgatttaa gatcggatct atatcgcaag gtgcaggagc ttcctcttac
     8521 attcttctac agtcagaagt caggtgtgat cctcagtaga atgatcaatg atgccgaagt
     8581 agtggccgct gtgatttcga gcaatctgag agatgcgatc acgaacgttt ttattctggt
     8641 gactcacttt gccgtacttg cctatctcaa tctgcagctc ttgcttgtat ctctgattgc
     8701 agtacctttg atgctttcgc ctgtgactct atttgcgcga aaggtaagaa agtctacagg
     8761 gaaaagccag gaatatctcg ccgatctcaa cgctcttcta aaagaaacga tctcaggcgt
     8821 gaaagtgatt cgttctttcg ccatggaagg ttacgaagca gaacagttcc gtcgcgcgaa
     8881 tcagaaactt tactggcgaa ctttcaagca acaattctat cttcgcgcat caccgaatct
     8941 tgtagaactg accagtgcag tcgttgcact cgggatgctc ggactgggag cgttctttct
     9001 agatccaact gattttaccg gcggccagtt catggcattc ctggtgattc ttctttctct
     9061 gatccggcca gtgatccagc tctccggtat gatggcgaaa gtggggcagg gatcggaagc
     9121 aggtagacgc atttttgaaa ttctggacag acccagagac atagaagagg cgcgacatcc
     9181 tgctgtgctt cgcccgttgc gaaagcatct tgagttcaaa aatgtttcct ttcagtatcc
     9241 tgaaacggac aaactcgtgc tcgaaaatat caatctccat gtagaggcgg gccagaccat
     9301 cgcactggtg ggagaaagcg gaagtggcaa gtccactctg atggatttgc tcgcgcgctt
     9361 ttttgatcca accagcggaa gcattcgaat cgatggaaca gacattcgcg actttcgcat
     9421 cgcagatcac agaagcagaa tcggaatcgt gcagcaggaa aattttctgt ttcatggtcc
     9481 cgtacgtgaa aacatagcgt atggacggcc cgagatttcg attcgcgaaa tcatgaaggc
     9541 cgcccggctt gctcacgctc acgacttcat caaagaattg cccggaaaat acgaatccat
     9601 gattggagaa cgagggctga acctttcagg cggtcagaga cagaggatcg ccattgcgcg
     9661 ggctcttcta cgcgatccgg aaattctcat tctggacgag gccacgtccg ctctggacac
     9721 ccagtctgag cgcctggtcc agcacgctct cgaccggctt ttccgcaatc gcacagtttt
     9781 tgtgattgca caccggcttt ctaccattga aaaagccgat ttgattgtcg tcttgtccgg
     9841 cggccagatt gtggacatgg gcaagcattc agacctgatg cagaagggtg gactgtacgc
     9901 acggctgcag gagatttcca ggcaggctgt tccaggtttg gcctgacttt attttcgagg
     9961 attgtagatt gaaaccagtt ttactcgtgg atgacaatga tcgctacgca gagatgctag
    10021 ataagtattt tagagacaga ggctacactc cggagcgcgc tttcaacgga aaggacgcac
    10081 ttggaatgtt caaagggaaa cctgcggact actatcgcgc gattgttacg gatatcacca
    10141 tggaaagcca gcttgccggg ctgtcttttc tctgggagat caagaagctt ggctttcacg
    10201 gcacaatcgt tgtggccagc acaggattcg atttccctct cgccatgcct ctttcgcgca
    10261 tggttttgac tggctgggga gtgcactatc ttgtgccaaa ggctcccatg aagcgggccg
    10321 gaatcattga attttatcca gtttccctgt tttcttcgac cctgaaggag tttcaggaag
    10381 tccaataatt gatttacaac caacttcaaa aaggctcaaa agactcatga cgaatttccg
    10441 ccgcgtactc atcatttcca gcctggcttc tttgctcact tgcgtgccgg ggattccagt
    10501 gaagattcct cgctatccag aaggcgtcat gggcgatcag ctcaagagcg tcatgggcgc
    10561 gaagcgcaag gtcggcgtgg ttgccgccag ccccaacccg aatctactgc agcaaatcgg
    10621 atatgaccag gactggtctg ccactgtgga aggtgccgtc acaacggaac tgacccagcg
    10681 cggtttctac accatgattg atacatcgag tcgcaagcaa agactgcgcg agctcgctta
    10741 cactcaaact ggtttgaccg gcgaacaaag aaacatcggc caggagctcg ctgcagaagg
    10801 actgttgttc attcgcatga ctgctcctcc tctcaaagaa tgcaaaactg aatacgtaac
    10861 cgatttcgcg gccacggctc tgaagcttgg aatgaagatg gccatgaaag attccaatgg
    10921 tgcgggagac gcgcaggaga aaaagccaac tggcgttttg ttcctcacag ttttcgtgca
    10981 gggcactctt acgaatctag aaacaggcca gtcggtaaca tacgcgcaca gcaaaccata
    11041 caaactccag aatgaggccg ggaatcagga atgcccatcc actctcgatg cgtttggaaa
    11101 agctctgcag gatgccagcg cggccatcgc agacaatctt tcgccttcta tcgtaactgt
    11161 gaagatccct cttgcatcgg atgcagatga cctggacgga gaccagaaag caaaggtgac
    11221 caaatatctg acggaaggaa tcaagtgggc agaagcagac gatttccaga tggcagcgca
    11281 aaactgggaa aaggcgctcg atgcgtccgg cggcaaatcg gcttccgctc tctggaacat
    11341 agcagcttat aagtggtatt ctggagatat ggacggagcg gagaaatact tcaaacgctc
    11401 cttctccaac ggtggatcag actgggcgga cgcggccaag agaagtctct gggctacgtt
    11461 ccgcaaagag aaagaccgta aggaaagcga gggcgggaat taagtcctcc g
//
LOCUS       DAOQJL010000049        11426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2864_length_11426_cov_4.401284, whole genome shotgun sequence.
ACCESSION   DAOQJL010000049 DAOQJL010000000
VERSION     DAOQJL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2864_length_11426_cov_4.401284"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLG78_03565"
     CDS             complement(<1..744)
                     /locus_tag="PLG78_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNE22215.1"
                     /translation="MEIGAIIITIAGLCLFETVSSIDNAIINAEVLGTMKPKYRRWFL
                     IYGIFIAVIVVRGALPWLIVWAVNPTLGPVGAFMASFSNDPHVVDSIQASAPILLVGG
                     GTFLILLFLHWLFLEPKNYGLYGERYIESQGVWFYAFASIALLVIVWLALKKDSMMAM
                     GAVTGSTAFFITHGFKENAEQAEQDLLTSHKSDIAKILYLEILDATFSIDGVLGAFAF
                     TLYVPLILIGNGLGAIVVRQLTVGNIERIK"
     gene            complement(735..2666)
                     /gene="asd"
                     /locus_tag="PLG78_03570"
     CDS             complement(735..2666)
                     /gene="asd"
                     /locus_tag="PLG78_03570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNE22216.1"
                     /translation="MNEIISYVSIPVISLILFAGLFLTIRFQGVQLRELLLSIKVLSG
                     MMDQPSSKEQHTMFQAFLLSLAGVIGGSVVLAGTHILDSRTWSWFLIFAFIYAPIQFS
                     VGATLSRNTRSIAVLAALALIGLQGVSQTSYTASIEMHLAWNPLAVGLIVTALSAGIV
                     FAPVRIGASIFAWSGLLFVIYVIRNAFVGGPGSLPGLYFSTNPVHISSALLAALTLCE
                     IARAPFLFVSVRSSGAKPALVSMLSVLLSPFVFAIVFYVLDAFIPSDVRTMSLAAIVG
                     LVLWTASWMWTIRSFVPGSKGTLLSLAFALLPLAMQHTSYSMMVDILVFGSAAGGLAL
                     ACFTVEAFLRSGEASQSLAELRESHRWEISKDIYLIFLTILPQNLVSRIFGWIAATPL
                     PGFLREPIILAYSRAFGVTVEEAAKEIREYSSLNNFFIRYLKPGARPIEGDEKTIVSP
                     VDGTVLRFGDITEGLLIQAKAMQYSLRDLLEFPDYLQDFEGGKFLVIYLSPRDYHRIH
                     FPVGGTVPAFAYSPGDLFTVNPVAVERLDALFAKNERLATYIHNPRGTVALIKVGATN
                     VGRIVLAYDSFKTNRWFRRPVFKRYATPVEAKRGDELGRFEMGSTVILLFGHNKIDFL
                     PIIQEKNKVKFGQGIAKWK"
     gene            2653..3549
                     /locus_tag="PLG78_03575"
     CDS             2653..3549
                     /locus_tag="PLG78_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22217.1"
                     /translation="MISFMMQSDAVLCRVGGNPVKAFCLSALVAWLIMSAPGLEAIPV
                     EIGISGVGSQRNPGTFDQGLDNYRTSLNPLVFSLTKHSNFRSIPASEGYIRLGDLISE
                     ESYIGLLYGRLYTPPISVRQFRSDAVYFQSNWNFFVHYFGFTYHYRFRPISSSRMSYE
                     VGGAYTFVAEPTWRTDGYYLSPYQFSRLQTSHRAGYGSMLRLEGAVVRGLGDHFFVRA
                     GIAQSYWYMGRYQGNVNSTGGYWVTLRNGGFLALAPSQFISTFVVSPDPILGPTQVST
                     IRDRADINFSLTQFFVSAGLRF"
     gene            3593..3832
                     /locus_tag="PLG78_03580"
     CDS             3593..3832
                     /locus_tag="PLG78_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22218.1"
                     /translation="MSAEKVITMPVREIADVKVILATWYKFLQDQADKLNAEEFSRSL
                     KTPIILNLETDELELLFTGSGELLQGFKDFILTTT"
     gene            complement(3840..4514)
                     /locus_tag="PLG78_03585"
     CDS             complement(3840..4514)
                     /locus_tag="PLG78_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNE22219.1"
                     /translation="MCGRFALFEEKEVLEGFFGFQIDLDFGPHYDIRPTDQVLSFAHH
                     GASLMRWALLPAWEKSNQVRYATFNARLEEAATKPAFRSSVIANRRCVIPASGFYEWK
                     DVAKKKKDRYYFRRLDGKPLAMAGLWDTWKGGGVEIVSCTILTTEANELIRPIHAKHR
                     MPVILDANEISEWLDPNTPSDSLFRKHATGFAAGRMETYKVDSRLREDDKRLVEKYEE
                     PELFKL"
     gene            4610..5095
                     /locus_tag="PLG78_03590"
     CDS             4610..5095
                     /locus_tag="PLG78_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22220.1"
                     /translation="MRSIVFAVLVAIIAPLMAQTKANDIPVEKLPADVKQVLEQYVNI
                     LRTSKSLDECATRFTSIAGGSLVTDEKGTLRDTVKPFSLKKDFNDIKQYADPIVITRV
                     NATPSNGQGYGSSAIKGMVYKIWIAKKDASIGMPAPVSILVPEGHPTITTPKVVGIGS
                     Y"
     gene            5145..6353
                     /locus_tag="PLG78_03595"
     CDS             5145..6353
                     /locus_tag="PLG78_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22221.1"
                     /translation="MYQLFIQIIKYGFSTVLFGVGLYVLIASYREHKSFRRLFKGFAI
                     TAYFLFAAGMTLLIFTGETAAQLSMFFIKAISTAFGLLYGIYATLNGFMETDPATGKK
                     TVTRLGVLGIFILSFNTILLVSMDYLQETAAMEEKLQEKQLQLDQERERKIEYERLLG
                     SVSFVDRKTGDLKIDFEKFSESLYRSLVTVETDLRNTRIELATRETELKDATARWNAA
                     DAGAKVLQETLSKTEKALATRDAELKESQTKLAQSDASLKAAQENLAKTEKSLAGREA
                     ELKNTSDQLVESQKEAKKAEEELSRTSRDLASKDAEAREQTRRATAAESSLARAQDEL
                     KKSAAAFTTRDAEARQYQKENLTLQTNLARETEERSKLDKLLAARDAELRACKTAPAT
                     PTPPQPVPPG"
     gene            complement(6371..6847)
                     /locus_tag="PLG78_03600"
     CDS             complement(6371..6847)
                     /locus_tag="PLG78_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE22222.1"
                     /translation="MFLSSPFPGGATFIGISGADSVCNNEPARPILIATYKAFIVSST
                     VRRASVTAGVGDGQIDWVLQPSTVYTRADGTLIAQTNSNSIFTFPLAASFSPSPGVIW
                     TGLAGNWTNGAADCSGWTGTATTALIGNANVTSNASISAAGNNCNTAGYMLLCVEQ"
     gene            7045..8280
                     /locus_tag="PLG78_03605"
     CDS             7045..8280
                     /locus_tag="PLG78_03605"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22223.1"
                     /translation="MLQLLWNTIAYSGSTHASHSSEVKHIVLLNALAMITAAFALFNL
                     PVTLFVHAPLSVICGTLAFSLLMPLVTIILNGRGHLLAARFWFLVVSSGRIVLGMIFL
                     GSVLPLHFFFLALMTVSFFVFPAKQKKLMIATCIVMLSSFIGFELFRDQIPLLAWMKP
                     EYIQIQRVAINAGFGILTFCIAFYIYSTFDRSERYLHAEQSKSERLLNNILPGSIVRQ
                     LRESPDTIAERFENCTVLFSDIVGFTSMSRQMPAVQVVELLNGIFSAFDDLAEKHGLE
                     KIKTIGDAYMIVGGLPEPDPHHAENVARFALEMLDVVRQYRQENGLPLELRIGINSGD
                     AVAGVIGKKKFIYDLWGDSVNTASRMESHGLPGQIQVTESTYMLLKDKFQFSERGSME
                     VKGLGIVKSYLLMEAMEPI"
     gene            complement(8282..8617)
                     /locus_tag="PLG78_03610"
     CDS             complement(8282..8617)
                     /locus_tag="PLG78_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22224.1"
                     /translation="MQIQSKEQDRYMLHRIEGEVDLYNSGVLKSALMNAFAANGKNIL
                     LDLSAMVYIDSTGMSVLFQTATQLREAKKDLKVFGATPAVMSVLSMVNVPGKLQICQT
                     EEEAAMFFR"
     gene            complement(8629..9060)
                     /locus_tag="PLG78_03615"
     CDS             complement(8629..9060)
                     /locus_tag="PLG78_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22225.1"
                     /translation="MEKTYLPFLIVAIVPLFWLILVTGIMYLISFTGWRNLAKKYANQ
                     GENPQTGFHWASVQTSWIGRYNNCINLAITPRGLFVQPWLLFRFAHPGLLMPWNMLKH
                     ARPRSVLFWDFAELEFDDGKDSLLVYAPSRILQAPGFPRQT"
     gene            complement(9095..9565)
                     /locus_tag="PLG78_03620"
     CDS             complement(9095..9565)
                     /locus_tag="PLG78_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22226.1"
                     /translation="MIKTLVVEKNFRGSMRDVFYDLAENPNYFPKYFKGFAPFVPGIR
                     KITPQWTGQIVPGGVRKVDLSDGSFVMERVTVHEPPRLQKYEMAELNSMQKLVCQKMH
                     AEYSLEDKGEFVHVKWTYWIETEKLLQKPLVAIVGWAFHKAMHRFMDAASSHAR"
     gene            complement(9638..9976)
                     /locus_tag="PLG78_03625"
     CDS             complement(9638..9976)
                     /locus_tag="PLG78_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22227.1"
                     /translation="MPLQTNQIGPNLIVYLDGNIDLSQSVLIETELLKLIEQHSSKNL
                     ILDFTQVSAMSSSGVRVLVLLRRTLKETKQKLALCNLGAPVLRIIKLLELDGRFEIFN
                     SQEDALRAHE"
     gene            10058..11167
                     /locus_tag="PLG78_03630"
     CDS             10058..11167
                     /locus_tag="PLG78_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE22228.1"
                     /translation="MRKILWTLLLAWLQLLACSAPAQCKIGDSSCALNSLPLLFSRTI
                     LARFLFSANQTGNNLNVYRISQATGQLTSVGTIAAGTYPTSVGSALDSRFVYVSNQNS
                     NDITQLIVDPISGAPTANGSLTIAGGSPQAILIPTGGQFGFSANYALNNLSNLVINSM
                     TGQLTQSSVTGAGTSPQGIVFDSTQSNLFSVNFNSNNVSCFSVTANGGLSLNATVSTG
                     GGSGPFEMVIHPNGRFAYVSLSTLGQVSVHSITNGVLSSSLATYGAGTSNKHIVIHPS
                     GSFVYAVNFGSSNISIFTVQSNGLLSGPTNVTTPANPLGFVFEPGGKYAYGAGGLSTI
                     TTYSVDSTTGSLTAIGTTNSGTGSSSLSVGLFSTF"
     gene            complement(11189..>11426)
                     /locus_tag="PLG78_03635"
     CDS             complement(11189..>11426)
                     /locus_tag="PLG78_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22229.1"
                     /translation="EWLFCELTNKKRVVLVIGAAQGQWVKAHLLDVLPTMDFVLQEQD
                     IFMVQGLRAKIQASGITDPDLQHKFAEFLQQAVR"
BASE COUNT         3063 a         2910 c         2757 g         2696 t
ORIGIN      
        1 cttgattctc tcgatgttcc caacagtcaa ctggcgcact acaatggcac ccaggccatt
       61 gccaatcagg atcaatggaa catacagcgt gaaagcgaaa gcacctaaca ccccgtcaat
      121 ggagaaggtt gcgtccagaa tttccaaata gaggatcttc gcaatgtctg atttgtgcga
      181 agtaagcaaa tcctgctctg cctgctccgc gttctcttta aaaccgtggg tgataaaaaa
      241 agcagtcgag cctgtaaccg cacccatggc catcatggaa tccttcttca gtgcaagcca
      301 gactataacg agaagtgcaa tcgacgcgaa tgcgtaaaac cagacacctt gcgattcaat
      361 gtatctctct ccataaaggc catagttctt tggctcaaga aacaaccagt gcaaaaacaa
      421 caggatgaga aatgttccgc cgcctaccag gagaatgggg gcagaggcct ggatggaatc
      481 cacaacatgt ggatcattcg aaaacgaagc catgaatgcg cccactggac caagggttgg
      541 attcacggcc cacacaatga gccagggcag agcgccgcgg accacgatga cagcaatgaa
      601 aatgccataa atcaaaaacc atcggcgata ctttggcttc atcgtgccaa gcacctctgc
      661 attgatgatg gcattgtcta tgctggaaac cgtctcaaaa agacagagac ccgcaatggt
      721 aatgatgatc gcgcctattt ccattttgca atcccctgtc caaacttgac tttgttcttt
      781 tcctggatga ttggtaagaa gtctatcttg ttgtgcccga acaataggat cactgtggac
      841 cccatttcaa atcgaccaag ctcatctccg cgtttcgcct ccacgggtgt cgcataacgt
      901 ttgaacacag gacgacggaa ccagcgattt gtcttaaatg aatcgtatgc aagtacaatc
      961 cttcctacat tcgttgctcc cactttgatc aaagccacag tgccacgcgg attgtgaata
     1021 tatgtggcca gtctttcgtt cttagcaaac aacgcgtcca atctttccac ggcaacgggg
     1081 ttcacagtaa aaaggtctcc cggagaatag gcgaaagcgg gaacagtgcc acccactgga
     1141 aaatgaattc tatgataatc gcggggactc agataaatca ccagaaactt tccgccttca
     1201 aagtcctgga gatagtccgg gaactccagc aaatcacgca gcgaatactg catggccttt
     1261 gcttgaatga gaagcccttc tgtgatgtct ccgaacctga gaacggtccc atccacagga
     1321 gacacaatgg tcttttcgtc tccttcgatg gggcgagcgc ctggtttcaa ataacggatg
     1381 aagaagttgt tcaatgagga gtactcacga atttcttttg cggcttcctc tacggttaca
     1441 ccaaatgcac gcgaatatgc caggatgatt ggttcgcgca ggaatccagg cagaggagtg
     1501 gctgcaatcc agccaaatat tctggagaca aggttctgag gcagaatcgt taagaatatt
     1561 agataaatat ctttactgat ctcccaacga tgagattcgc gaagctcggc gagtgattga
     1621 gatgcttcgc cgctacgaag aaacgcctct actgtaaagc atgcgagagc cagaccgccc
     1681 gccgcggatc caaacacaag tatgtccacc atcatggagt aggaagtgtg ctgcatggca
     1741 agtggcagaa gcgcgaaggc taaagaaaga agagtgccct ttgagcctgg aacaaaagaa
     1801 cgaatggtcc acatccagga agcggtccag aggaccagac ccacaatggc cgcaagactc
     1861 atagtgcgga catcagaagg aatgaacgca tctaacacgt agaatacgat cgcaaacaca
     1921 aaaggagaaa gcaaaaccga aagcatggac acaagcgccg gctttgctcc ggacgagcga
     1981 acagatacaa aaaggaaggg ggctcgggca atttcgcata acgtcaacgc cgcgagcaga
     2041 gctgaagaaa tatgaaccgg attggttgaa aaatacagtc ccgggagaga acctgggcca
     2101 ccaacaaagg cgtttcgaat cacgtatatg acaaacaaaa ggccggacca ggcgaaaatg
     2161 gaagccccga ttctcacggg agcaaaaaca atgccagccg aaagcgcggt cacaatcaaa
     2221 ccgactgcaa gcggattcca tgcgagatgc atttcaatcg aagccgtata agaagtctga
     2281 gagacaccct gaagaccgat gagcgctaga gcggctaaca ctgcaatcga acgagtgttg
     2341 cgcgacaggg ttgcgcccac gctaaattga atgggtgcgt aaatgaaggc gaagattaag
     2401 aaccaggacc acgtgcgaga gtccagaatg tgagttccgg caagtacaac gcttccccca
     2461 attacgcctg cgagagaaag caagaacgcc tggaacattg tatgttgctc tttgctcgat
     2521 ggttgatcca tcataccgct gagcaccttg atggacagaa gcaattcgcg tagctggacg
     2581 ccctggaatc gaatggtcag gaaaagcccc gcaaatagga tcagcgaaat gacggggatc
     2641 gatacgtaag aaatgatttc attcatgatg caatcggatg cagttctatg ccgcgtggga
     2701 ggcaacccgg tcaaggcatt ttgcctgagc gcactggtcg cgtggctcat catgagcgcg
     2761 cccggactgg aagcaatccc tgtggagatt ggaatcagcg gggtcgggag tcaaaggaat
     2821 cccggcacat tcgaccaggg gctggacaat tacagaacat ccctcaaccc actggtgttc
     2881 tcgcttacga agcattcaaa cttcagaagt atccctgcct cggagggtta cattcgtctg
     2941 ggagatctga tatcggaaga aagttacatt ggccttctct atgggagact ctacactccc
     3001 ccgatttcag tgcggcagtt tcgtagtgat gcagtctact tccagtccaa ctggaatttc
     3061 ttcgtgcatt actttggttt tacataccac tatcgctttc gcccgatttc cagctctcgc
     3121 atgtcctacg aagtcggagg agcgtatact tttgtggcag aacctacatg gagaacagac
     3181 ggatactatt tgagtcctta ccaattcagt cgccttcaga ccagccatcg cgcgggatac
     3241 ggtagcatgc tacgccttga aggggcggtg gttcgtggcc tgggagatca cttcttcgtt
     3301 cgcgcgggca tcgcgcaatc ttactggtat atgggtagat atcagggcaa cgtaaattcg
     3361 acgggaggtt actgggtgac attgcgaaac ggcggttttc tggctcttgc tccttctcag
     3421 ttcatttcca ccttcgtggt aagcccggat ccaattctcg gaccaacgca ggtaagtacg
     3481 attcgggacc gggcggacat aaatttcagt ctaacccagt tctttgtcag tgcgggcctc
     3541 agattttgat ttacaacatc cgactctcct aatatcccat gtcagggttc ccatgagcgc
     3601 agaaaaagta atcaccatgc cagtacgtga aatcgcagat gtgaaggtca ttctggccac
     3661 ctggtataag tttctgcagg atcaagcgga caagctgaac gcagaggaat tctctcggtc
     3721 gcttaagact cccatcattc taaacctgga aacggacgaa ctcgaactac tcttcaccgg
     3781 atccggagaa ctccttcagg ggttcaaaga tttcatactc actacaacct gagtgcttct
     3841 cacagcttaa acaattcggg ttcttcgtat ttttccacca ggcgtttgtc gtcttcgcgc
     3901 aacctggaat ccactttgta tgtttccatt ctccccgctg caaatccagt ggcatgtttg
     3961 cgaaacaaag agtctgatgg tgtgttaggg tctaaccatt ccgaaatttc atttgcatcg
     4021 agaatcacag gcattctatg cttcgcatga atcggacgaa tcaactcatt tgcctccgta
     4081 gtcaagatcg tgcatgaaac gatttctact cctccgcctt tccacgtatc ccagagaccg
     4141 gccatggcca gaggctttcc atccaatcga cgaaaatagt atcgatcctt tttctttttt
     4201 gccacatcct tccattcgta gaatccactc gctggaatca cgcaccgtcg attcgcaatt
     4261 acactcgacc gaaacgcagg tttagtggcc gcttcttcca accgggcgtt gaaggtagcg
     4321 tatctgactt gatttgattt ctcccaggcc ggaagcagag cccatcgcat cagagacgct
     4381 ccgtgatgcg caaaagacaa aacctgatct gttggccgaa tgtcgtagtg cggcccgaaa
     4441 tccaaatcga tctgaaaacc aaaaaatcct tcgagcactt ctttctcttc gaaaagagca
     4501 aatcgtccgc acatccggga agcatgctct cgtggctttt tttcgcaacg atatttgaag
     4561 ttagtcgaaa cctgttgacc catggaacac cgacccgcgt ctaaggtgca tgagatctat
     4621 cgtattcgct gtcctggttg cgatcatagc gccgctgatg gcacaaacaa aagcaaacga
     4681 catccccgtc gagaagctgc ctgcagacgt aaaacaggtt ctcgaacagt atgtgaacat
     4741 attgcgcacg agcaagagcc tggacgaatg cgcgacccgc ttcacttcca ttgccggtgg
     4801 cagtctggtt acggatgaaa aaggaaccct tcgcgatact gtgaaaccat tttccctgaa
     4861 gaaggatttc aatgacatca agcagtacgc ggatccaatt gtaatcactc gcgtgaatgc
     4921 aacgccgtcc aatggacagg ggtacggatc gagcgccatc aaaggcatgg tgtacaaaat
     4981 ttggatcgcg aaaaaggacg catccattgg aatgcctgcc cccgtatcga ttctggttcc
     5041 agaagggcac cccaccatca ccacacccaa agtggtcgga attggaagtt actgacctcc
     5101 ggcgcccgaa agggcgcctc gattcttaat cgccggataa cgttatgtat cagctattca
     5161 ttcaaattat caaatacggt ttcagtactg ttctgtttgg agtaggactc tacgtcttga
     5221 tcgcatccta ccgcgagcac aaatctttca gacgcttgtt caaaggattc gcgatcactg
     5281 catacttctt gtttgccgca ggaatgactc tgctaatttt cacaggcgaa acggcagcac
     5341 agttgagcat gttcttcatc aaggcaatct ccactgcgtt cggattgctg tacggcattt
     5401 acgcaactct caacgggttc atggaaacgg atccggccac cggaaagaag accgttacgc
     5461 gacttggggt cctgggtatt ttcatcctca gctttaacac gatcctcctg gtcagcatgg
     5521 attaccttca agaaaccgcc gccatggaag aaaaactgca ggagaaacaa ctgcaactgg
     5581 accaggaacg cgaaaggaaa attgaatacg aacgccttct tgggagcgtc agtttcgtgg
     5641 atcgaaaaac aggcgatttg aaaatcgact ttgagaagtt tagcgaaagc ctgtacagat
     5701 ccctggtcac agtggaaaca gatctccgca ataccagaat tgaactggca accagagaaa
     5761 cagaattgaa ggacgcaacg gctcgatgga atgcagcaga cgccggcgca aaagtcttac
     5821 aagaaactct ttcgaaaacg gaaaaggccc tggccactcg cgacgcggaa ttaaaagagt
     5881 cgcagactaa actggcccag agcgacgcca gcttgaaggc cgcgcaagaa aatcttgcta
     5941 aaacagagaa gagcctcgca ggacgagaag cagaactgaa aaatacttca gatcaacttg
     6001 ttgaatcgca aaaggaagca aagaaagcag aagaagagct aagccgaacg tcacgcgatc
     6061 ttgcaagcaa agatgcggag gcacgtgaac aaactcgccg ggccactgcc gcagaatcat
     6121 cccttgcgcg tgcgcaggat gagttaaaaa agtccgctgc agcattcact actcgcgatg
     6181 cagaagcccg ccagtaccag aaagagaatt taactctaca aacaaatctt gcgcgcgaaa
     6241 ccgaagagcg cagtaaactg gacaagcttc ttgcagcgcg cgacgcagaa ctgcgcgcat
     6301 gcaaaacggc accggcaact ccaacgccac cacagcctgt gccaccgggc taatccaccg
     6361 cgaacgatgt ctactgttct acgcacaaga gcatatagcc cgctgtgttg cagttgttgc
     6421 ctgcggccga aatagacgca tttgaagtca cgtttgcgtt tccaatcaga gcagtggtcg
     6481 cagttccggt ccaaccagaa caatcggccg ctccgttagt ccaatttcct gccaggccag
     6541 tccagatcac ccccggcgat ggcgaaaaag aagcggcaag cggaaatgta aaaatgctat
     6601 ttgagttggt ctgcgcgatc agtgtgccat cggcgcgcgt atatacagta gagggctgga
     6661 gcacccaatc gatctgccca tcccctacac ctgctgtgac actcgcacgt cggactgtgc
     6721 ttgagacaat gaatgctttg taagtcgcga tgaggatggg tcgagcaggc tcattgttac
     6781 aaacgctgtc tgcgcctgaa atgccaataa aagtggctcc tcctggaaaa ggagaactca
     6841 ggaacatctg acaacttccc cggatcgcgc aacgaagacg agtgaacagg aaggtcagag
     6901 ccggctggca agtaaagtca gcactatcac aggcgttgga gtacgaacac gaaatagcag
     6961 aagctgcaat aaaagttacg aacaaacagc gaatcagcaa ttttttgaga tgcgctttca
     7021 acgcctttcg cctttcttcc gtgtatgctc caactgctct ggaatacaat tgcatactca
     7081 ggtagtactc acgccagcca cagctcagaa gtaaagcaca ttgtcctact caatgctctg
     7141 gccatgatca cagctgcttt tgcgcttttc aatttgcctg tcactctatt tgtgcatgct
     7201 cccttgtctg tcatctgcgg cactctggcc ttctctttac tcatgcctct ggttacgatc
     7261 attctcaatg gccgcggcca tttgctggcg gctcgattct ggtttctagt tgtatcttcg
     7321 ggaagaatcg tacttggaat gatcttcctg ggaagtgtgc taccgcttca tttctttttt
     7381 ctggccctca tgacggtctc gttcttcgtt ttcccggcaa agcagaagaa gctaatgatt
     7441 gcaacctgca ttgtaatgct ctcttctttt ataggatttg aactttttag agatcagatt
     7501 ccgcttctgg cgtggatgaa acctgaatac atccagatcc agagagtcgc catcaatgcc
     7561 ggatttggaa ttcttacgtt ttgcatagca ttttatattt acagtacttt tgatcgctct
     7621 gaacgttact tgcacgctga acaatcaaaa tctgaaagac ttctcaataa cattttgcct
     7681 ggatccattg tacgacaact gagagaaagc ccggatacaa tcgcagaacg atttgaaaac
     7741 tgcaccgtac ttttctcaga catcgtaggc tttaccagta tgtccaggca gatgcccgcg
     7801 gtgcaggtag tggaactcct caacggaatc ttttctgctt tcgatgatct ggcggaaaag
     7861 catggacttg agaaaatcaa aacgatcggg gatgcttaca tgatagtggg agggcttcca
     7921 gaacccgatc cgcatcatgc tgaaaacgta gcacgctttg ccctggaaat gctcgatgta
     7981 gtgcgccagt acagacaaga gaatggtctg cctctagaac tccgcattgg aatcaattcc
     8041 ggagatgcgg tggccggcgt aattggaaaa aagaaattca tttatgatct atggggagat
     8101 agcgtaaaca ctgcttctcg catggaatcg cacggacttc cgggccagat tcaggtcacg
     8161 gaatctacgt acatgctact gaaagacaaa tttcagtttt ccgaaagagg atccatggaa
     8221 gtcaagggac ttggcattgt gaagagctat ttgctaatgg aagcaatgga accgatatag
     8281 gttagcgaaa gaacatggcc gcttcttctt ctgtctggca gatttgaagc ttgcctggaa
     8341 cattcaccat gctcagcacg ctcatcacgg ccggtgtcgc gccaaaaact ttgaggtcct
     8401 tctttgcttc gcgcaattga gtggcagtct ggaacaaaac ggacattccg gtggaatcaa
     8461 tgtacaccat cgcagataaa tcgagcaaaa tgtttttgcc gttagccgca aatgcattca
     8521 tcagtgcact cttaaggacc ccagaattat agagatccac ctcaccttcg atgcggtgca
     8581 gcatgtatcg atcctgttct ttgctttgaa tctgcatgtg ctattccttc acgtttgcct
     8641 gggaaatcct ggcgcctgta agatccgaga aggtgcatac accagcaagg aatctttgcc
     8701 atcatcaaat tcaagttctg cgaaatccca gaaaagcaca gagcgagggc gagcatgctt
     8761 gagcatgttc cagggcatca gcaatcctgg atgagcaaac cgaaagagca gccagggttg
     8821 cacaaacaaa ccccgcggag tgatggccag attgatgcag ttgttgtatc tgccaatcca
     8881 ggacgtttgc acgctggccc agtgaaaacc ggtctgtgga ttttcaccct gattggcata
     8941 ctttttggcc aaattacgcc atccggtaaa cgatatcaga tacatgatcc cggttacaag
     9001 aatgagccag aacaaaggta caatggctac gatcaaaaac ggtaagtatg tcttttccat
     9061 ttgttactcc ggtcttgttt tccttttagg cagcctatct ggcatgcgat gatgctgcgt
     9121 ccatgaacct gtgcattgct ttgtggaagg cccaaccgac aatggcgact aacggttttt
     9181 gaagaagctt ttctgtttca atccagtagg tccatttcac atgaacaaat tcgcctttgt
     9241 cttccaggga atactctgca tgcatcttct ggcaaaccag tttttgcatg gaattgagtt
     9301 ccgccatttc gtatttttgc aacctgggag gttcatggac tgtgacacgc tccatcacga
     9361 aagaaccgtc gctcaagtcg acttttcgga ccccgcccgg tacaatttgt ccagtccatt
     9421 gaggcgtgat ctttcgaatt cctggaacaa aaggggcaaa ccctttgaaa tatttcggaa
     9481 agtagttcgg gttctctgcg agatcataga aaacatcgcg catactgcca cgaaaattct
     9541 tttctacgac gagtgtctta atcatagctc aagatgcccg ggacgtgaaa actgcataga
     9601 acaaacctgt cgttctgcga aagtgtcggc atgcatttca ttcatgagca cgcagagcgt
     9661 cttcctggga gttaaagatt tcgaaccgtc catctaattc gaggagtttg ataatacgca
     9721 gcacgggcgc gcccaggttg caaagtgcaa gcttctgttt tgtttctttg agagttcgcc
     9781 gcaaaagcac aagaacgcgt acgccagagg aggacatggc actcacctgg gtgaaatcca
     9841 ggatgaggtt cttgctggag tgttgctcaa tgagcttcag tagttcggtt tcaatgagaa
     9901 cggactggga gaggtcgata tttccgtcca ggtaaacaat caagttgggt ccgatctgat
     9961 tcgtctgcaa gggcatggaa tatttcggcc ggccgcggca tctccgtcat gcgaaatttt
    10021 ttttgcaagg gaaggcgcac aaaactgtta ataataggtg agaaaaatcc tctggacact
    10081 ccttctggcc tggcttcaac tcctcgcctg ctcggcaccg gctcagtgca aaatcgggga
    10141 ctcctcctgc gcgctgaatt cccttccgct gctgttttca cgcaccatcc tggcacgatt
    10201 tctgttttct gcaaaccaga ccggaaacaa cctgaacgtc tacagaatca gccaggccac
    10261 aggccaattg accagtgtcg ggacgattgc tgcaggcact tatccaacgt ccgttggttc
    10321 tgccctggac agcaggttcg tttacgtgag caaccagaac tcaaacgaca taacccagtt
    10381 gattgttgac cccataagcg gagcacccac ggccaatggt agtctaacga tcgccggcgg
    10441 ttctcctcag gccatcctca ttccaacggg tggtcagttt ggattctcag ccaactatgc
    10501 gctgaacaat ctgtccaatc tggtcataaa ttctatgact ggacagctca cgcagtcctc
    10561 tgttacagga gcgggtactt cgccgcaggg aattgttttt gattcaacgc agagcaactt
    10621 gttctccgtg aacttcaact caaacaacgt tagctgcttt tcagtcacag cgaatggcgg
    10681 cctttcactg aacgcaaccg tgagcacagg aggaggcagt gggccttttg aaatggtaat
    10741 tcatccgaac ggccgctttg cctacgtttc attgtccact cttggccagg tgtcggttca
    10801 tagcattacg aacggagttc tatcctcttc actcgcgacc tacggagcgg gcacttccaa
    10861 caagcacata gtgattcacc catctggttc ttttgtgtac gcggtgaatt ttggctcatc
    10921 gaacatttcc attttcacgg tgcaatccaa tggacttctc tcagggccga ctaacgttac
    10981 cacccccgca aatccgctcg gattcgtatt cgagccaggc ggtaaatacg cgtatggggc
    11041 aggcgggctt tccacaatca ctacttactc ggtggattca acaacgggtt cactcactgc
    11101 gatcggaact accaactccg ggacaggatc aagttctcta tccgtgggtt tattcagcac
    11161 attttagcgg aacggtggag aacgccgcct aacggaccgc ctgttgcagg aattcggcaa
    11221 atttgtgttg taaatcagga tctgtaattc cgctggcctg aatcttcgcg cgcaaccctt
    11281 gaaccataaa gatatcctgt tcttgcaaaa cgaaatccat ggttggcaag acatccagca
    11341 gatgtgcttt gacccactgg ccctgcgctg cccctatcac cagaacaacg cgcttcttat
    11401 tggtgagttc gcagaacagc cattca
//
LOCUS       DAOQJL010000050        11409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2872_length_11409_cov_4.430333, whole genome shotgun sequence.
ACCESSION   DAOQJL010000050 DAOQJL010000000
VERSION     DAOQJL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2872_length_11409_cov_4.430333"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLG78_03640"
     CDS             complement(<1..238)
                     /locus_tag="PLG78_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE22230.1"
                     /translation="MKNKTVLITGSTDGIGVQTGLELAKQGARILIHGRNPDRAAKVV
                     SQIESAGGQAQSLIADLSSMADVKKLAAAANAAGP"
     gene            complement(457..1281)
                     /locus_tag="PLG78_03645"
     CDS             complement(457..1281)
                     /locus_tag="PLG78_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE22231.1"
                     /translation="MLKIPRPDAQQLVGLHGFADTGETFQFVQAPLANRFELLFPDMR
                     GHGDSSRLRESYYGAHSYFGDLIRFTGTVKKPYVLMGHSMGAAMAARFAGLYPDEISH
                     LILLEGFSGISSEEEEIRRLRSFGDNLRKKRPEKVRILKTKREAELILGTVHEGLPPE
                     RISALAEYLAAPVEGGFVWKQDPEVKTGGIPIPFSPAFSRALWKRITCPVLFIYGERS
                     HLLPGKRTNSSGSLDEILSHFQNLETHSLDSGHNPHHEKPEEVMQLLEAFFQKHPG"
     gene            complement(1322..1873)
                     /locus_tag="PLG78_03650"
     CDS             complement(1322..1873)
                     /locus_tag="PLG78_03650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE22232.1"
                     /translation="MKWILTNEEDLLSTVPFTVQRLSLVDRVRGPLHDFHRLRAPDWA
                     NILPITREGEALLIRQPRAGAMELILESPGGAVDAAETDPLVAAARELEEETGYTSER
                     MTHIASFNPNPALFSNKIHFFLAEGCFINPNREHFPDSSEDIEIVLVPLDELEERVAR
                     GEINHALAALGIMLSLRRLKSGN"
     gene            complement(1873..3012)
                     /locus_tag="PLG78_03655"
     CDS             complement(1873..3012)
                     /locus_tag="PLG78_03655"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNE22233.1"
                     /translation="MKFAAFTILAFFVSISAIQAEQIIMKTGQVYNGRIVNQTRNEVI
                     LDTAAGRVVLRKIDIRRITYGPTPEDQTRDARKREEQKKAEEDKKKEDQKLAEEKKKA
                     EEDKNRADQKLADEKRQAEEKQKAEELKKIEDSKKSEEQKAADADRLKKEEEARKAAE
                     EKKQAELLKQQEELKKSEEKKTDELRVTEEQIRERVRQEVQLELQKQEQRRREEERRR
                     LEEEAKRQSQPTRSGAFFRSLVLPGWGQLYQGRRIAGFAYMGGVLGAAAVSNYQDQIY
                     KRRLGEYHDITNTFLFSTPFVTRLLGYAQDDYQASTVFFLGTDQTQKAYNGMAKAARR
                     GRWARGVALGLYAGNLVDVLIFAPRASVGVTPSQDGFNLAFHYQF"
     gene            3077..4144
                     /locus_tag="PLG78_03660"
     CDS             3077..4144
                     /locus_tag="PLG78_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22234.1"
                     /translation="MSYSIQDAKPGLVAFKDSPVGRNILGGCLLAVGLLLSLGALAKI
                     LTGPLAGGPPLIGMSILILSGSILVITQIRPPKFLLLDDAKGALIICEKTMDQPTALI
                     PYSAFEEIRTARGLPSRNRRRTFGVDLVKKDGVRYAIFETRTLQAAEEATALLKKNID
                     LRSQDNASRIVSSTFRRENKNDAIKIEWRMPVRVLRTILSAAMATGFSILLIESYDSV
                     PVLFWPLQIFLCAIAVLSLAYLFYPRKQVVTIDRIQLKAEVTGIFAKQLIVPLSEIAS
                     VQFQFAAAWEDTIYLLNEHETAVMRAYQEGTLDPQRALEIGKMMLQARRIPSGGLTAS
                     EMISLEQILEETLTDQGRAVQ"
     gene            4141..4884
                     /locus_tag="PLG78_03665"
     CDS             4141..4884
                     /locus_tag="PLG78_03665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE22235.1"
                     /translation="MSFKDHFSNHSRIYSEFRPDYPDAFIEWVCSLPEKKNLAWDCGT
                     GSGQAAVQLKKHFNRVIATDPSEEQIKHAKQLEGIEYRVLPAEKTNLDTASVDLICVA
                     QALHWFDFDLFFAEARRVLKPRGILCAWSYGLLETEEPVQTIIESFYRETVGPYWPAE
                     RRHVDERYATIPFPFYKIEAPYFQIERTWDMDSYLGYVSTWSAVQRMRKATGIDPLIE
                     LRNQLRPFYPGPLLIRWPIFALTGRAGNA"
     gene            complement(5063..5509)
                     /locus_tag="PLG78_03670"
     CDS             complement(5063..5509)
                     /locus_tag="PLG78_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22236.1"
                     /translation="MKKILIIAVAAFVVSCSKPAPAPETWNCDFKGQYVEKGETVPFT
                     WKVTWANTQGDNWKITGAAAEADAKSSTTGTCDAKTCKIDETYTAGENKGKSFYWVGT
                     YTDAETKNENVLITSFTGTFGPTDADRTSMGTWSAKADCKNANPAK"
     gene            complement(5613..6674)
                     /locus_tag="PLG78_03675"
     CDS             complement(5613..6674)
                     /locus_tag="PLG78_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE22237.1"
                     /translation="MRFTPYLLLLLILVLDQPVNLPWVHNAGRPEPTEHELLQTYVHN
                     ASAVLDADRQVGKKHVLLIGSSRSMMLGEIRHHDLNADPLLDDRSRATLREFTFDPRL
                     AFPGANLLGELIVADELTRSGVKPDLIVLELGPEFLNEVLVESSYNQNIYETDFLLRH
                     MKSFPREMMLDAIVRIALPSYRYRFRPEAAILGGFDSEFESKRNLMSLLLGQKRSFVS
                     EGREVRDYAGSDEIHDEAFRAQLDSIAQTLVPVRQRHHLDAMVVHLLGILKNSGIPTV
                     VWYPPVNPQFRQTIYERHGKELDPAGFDRIVRESGLAVADSTSFEKKCNRWEDAVHPS
                     RRCNGLIAMAILNAAAAQR"
     gene            6799..7212
                     /locus_tag="PLG78_03680"
     CDS             6799..7212
                     /locus_tag="PLG78_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE22238.1"
                     /translation="MTFLKKYTSLFLKFGLVGALGTVINLAVFWLLVEVLRIEPNIGS
                     VAAFVTALTANYFLNQNWTFREEAGGELFTAGSYARYAGVNLVGLAVNLIVLNAAIWL
                     FHPQGLMLVQAAGIAAGTIFNFVLSKLFVFKAEQD"
     gene            7215..8369
                     /locus_tag="PLG78_03685"
     CDS             7215..8369
                     /locus_tag="PLG78_03685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE22239.1"
                     /translation="MTTEKRPGLWQSLSGAHPGELPALDIVRAAAILMVFAYHAWTST
                     RTAVPAVAQVVDNLLLNGKSGVTLFFVLSGYLITSVLIREYATSANISLSQFYLKRSL
                     RILPAYYAYLIFFLILAVIGSHTFARGTENQFQRGWYFDFLFVSNIWQGFHVHTWSLA
                     TEEQFYLLFPVLAIALFRASKRIRIAIILALYLVPLAIRVVAFSKMEPALYGDWAYHR
                     PWTRFDDLLAGILVASAEISTMRAIYRRTILVVCSVAALSVLATSPSEFTWISPFLNN
                     GLNLSFAGIVLAAQASPLKKAPVFFTVTARLSYSFYLWHMLAGGIGAAVVFHSVKPGD
                     TVTWMKFARGILSAAFWSYVIALVSYRLIEMPFLRLRAPLLLRMQKKSST"
     gene            complement(8443..10608)
                     /locus_tag="PLG78_03690"
     CDS             complement(8443..10608)
                     /locus_tag="PLG78_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22240.1"
                     /translation="MRNLFNLAIAVLFTAAVGCTGTVGPASHGNGNMLTLFGVAALYH
                     GGSPIPTNEWASTGSHASNAITASEGGYVELGRNVKLAVPAGALDQDEYITVTAIDRP
                     AAADGVTPVLMGYKFEPEGLQFSKSALLTICYPSQELSSQGLSASTAQVYLQDEDGLI
                     GMGGSESGGCVTASVDHFSTYLAGAQALVAGSSAPSVSATANFLPGTPVAGIPLRIRT
                     IITSFQGTSTGSIAAAYVFWRTVGAPTYTQSVLTPDPTDAATPNRYTFLIPASAVTTT
                     GIQYYFRAIDNLGKITTTPVRTRTIARTATGITLNPAATLNIASGFSRDYTLSVTSDV
                     GGAQNISADTFNVGGGVGSAVKVSPSGIRFTAQGVGNGNLNVTAGSFSFSSPINVHPG
                     QLVNVTILDQSSVAITGTLNLSVNQVYAFDAIGRDAWGNVIPVLPSFGVTGPIGTIGT
                     SSGIFTASPTAGVSGTVDATLAGMTSSAQVTILSNAKALTAFSFPSLGATGVITGTNI
                     AITVPAGTNVSALTASFTTTGVSVKVGSTVQTSGSTPNNFAAPVTYTVVAEDGSFQDY
                     VVTLSNGPSVAANGMGPPNELQYCNLQWPYTMSVQTNQPTQNVYGRVYGQNVTEVAGP
                     NPIVLADFGYGPLGSDPINQPGLWTFVTAVYNVQIANDDEYMKSIIAPAPGSYAYVFR
                     FSMDGGAHYTYCDIDGAGYNPFPTNSFSPAQMGVMTVTP"
     gene            11109..>11409
                     /locus_tag="PLG78_03695"
     CDS             11109..>11409
                     /locus_tag="PLG78_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE22241.1"
                     /translation="MTILHPTKFDFSPLDPASSEIMQKTVQFFENKGLAKVKHDDHER
                     VWYADFLEFIKQEKIFYRLMTPAQYGDSPDVRFDSYRNNYFNEILGFYGLAYWYTW"
BASE COUNT         2750 a         2962 c         2824 g         2873 t
ORIGIN      
        1 tgggcccggc cgcattggcc gccgccgcca gcttctttac gtccgccatg gaagacaaat
       61 cagcgatgag gctttgtgcc tgaccgcccg cagattcaat ttgagaaaca acttttgccg
      121 cgcgatccgg gtttctgcca tgaatgagaa tgcgagcccc ctgtttcgcc agttcgagcc
      181 ctgtctgaac gccgattcca tctgttgagc ctgtaatgag tacggttttg tttttcaccg
      241 ggagaatata acaaatgttc tctgtaaggc actcgatttt tctatggtca ggagaacgag
      301 aacatcgccc gggcgacaga gaatgggcgc gaatccaggt gaggatttct cgccctacaa
      361 atggcgattt cgcgccgcga ttctatattt tttcgaatag cagttcgcga gaaatcttca
      421 acgaatgcgc ggcccgatag gcgacattgc ggttcttcac ccaggatgtt tttgaaaaaa
      481 agcttcgagc aattgcatca cttcttccgg tttctcgtga tgcgggttgt ggcctgagtc
      541 cagagaatgt gtttccaggt tttgaaaatg gctcaggatt tcatccagag atccggatga
      601 gttcgtgcgc ttgccgggca aaagatgact gcgctcgcca tagatgaaca atacaggaca
      661 agtgattctc ttccagaggg cacgactgaa tgccggactg aacggaatgg gaatgccgcc
      721 tgttttcact tccggatctt gcttccagac gaatccgcct tccacgggtg ctgccagata
      781 ttcggccagg gcggaaattc tttcgggcgg taaaccctcg tgcaccgtgc caagaatcag
      841 ctctgcctct ctctttgttt tgagaatgcg aactttttcc ggtcttttct ttctcaaatt
      901 atcgccaaag cttcggaggc gtcgaatctc ttcttcctcg ctcgaaatac ccgaaaatcc
      961 ttccaggaga atcaaatgcg aaatctcatc cggataaagc cctgcaaaac gagctgccat
     1021 cgccgcaccc atgctgtggc ccattagaac gtatggcttc ttcacagtac cggtgaatcg
     1081 aatcagatct ccaaaataag aatgtgctcc gtagtagctt tcgcgaagac gagatgaatc
     1141 gccatgaccg cgcatgtccg ggaacagcaa ttcaaatcgg tttgcgagtg gggcctgtac
     1201 aaattgaaaa gtttcccccg tatctgcgaa accatgcaga cctacgagtt gttgcgcgtc
     1261 cggtcgcgga atcttcagca cggacagatg gccgcctggc aattcgaatt gatagcgttc
     1321 cctagtttcc agactttaac ctgcgaagag aaagcatgat cccgagagcc gccagcgcat
     1381 gattgatttc tcccctggcc actctttctt ccagctcgtc gagcgggaca agaacgattt
     1441 caatgtcttc cgatgaatcg ggaaaatgct ctctattagg attgatgaag catccttccg
     1501 cgaggaagaa atggattttg tttgaaaaaa gggccgggtt tggattgaaa gaggcaatgt
     1561 gcgtcattcg ttcacttgtg tagccggttt cttcttccag ttctcgcgcg gcagcaacaa
     1621 gcggatctgt ttccgccgca tccacagcac cgcccggaga ctcaagaatt agttccatcg
     1681 cgcccgcacg tggctgccgg atgagaagcg cctcaccttc gcgcgtaata ggaagtatgt
     1741 tcgcccagtc aggtgcacgg agtctgtgaa aatcgtgtaa cgggccgcgc accctgtcca
     1801 caagagaaag cctctgcaca gtaaacggga ccgtgctgag gagatcttcc tcgtttgtaa
     1861 gaatccattt catcaaaact gatagtgaaa cgcaagattg aaaccgtctt gcgaaggtgt
     1921 cacaccaaca gaagcacgag gagcaaagat caatacgtca acgaggttgc ctgcatacaa
     1981 tccaagcgcc acgcctctgg cccaacggcc tcgacgggcg gcctttgcca taccgttata
     2041 cgctttctgt gtctgatccg ttccgagaaa gaagaccgtg gaagcctggt agtcatcctg
     2101 cgcgtatccg agcagtcgag tcacgaatgg agtagaaaaa agaaatgtat tggtgatatc
     2161 atgatactca cccagtcgcc gcttgtatat ctgatcctga tagttcgaga cagcagcagc
     2221 gcccagaacc ccgcccatgt atgcgaagcc ggcaatgcgt ctcccctggt acaactgccc
     2281 ccaacctggc aacacgagag atctaaaaaa ggctccggat cgggttggct gtgattggcg
     2341 tttcgcttcc tcttccaggc gtctacgctc ttcttcgcgt cgacgttgtt cttgtttttg
     2401 aagctccagc tgcacttcct ggcggactcg ttcccgaatc tgctcttccg taacgcgcaa
     2461 ttcatcggtt ttcttttctt ccgacttctt taactcttcc tgctgtttca acaattccgc
     2521 ttgcttcttt tcttctgctg ctttgcgggc ttcttcttcc ttcttcaggc gatccgcgtc
     2581 tgctgccttc tgctcttcag atttttttga atcttcaatt ttcttaagtt cttctgcttt
     2641 ctgcttttct tccgcctggc gcttttcgtc tgcaagcttc tggtctgcgc gattcttatc
     2701 ttcttccgct tttttctttt cttccgctag cttctgatct tccttcttct tatcctcttc
     2761 cgcctttttc tgctcttcgc gcttcctggc gtcccgggtc tggtcttccg gggtcggacc
     2821 gtacgtaatc ctgcgaatat caatctttcg caacaccacc ctgcccgccg cagtatcaag
     2881 gatcacttcg tttcgggtct ggttcacaat gcgaccattg tagacctgac cagttttcat
     2941 aatgatctgc tccgcttgga tcgcagaaat cgaaacaaaa aacgcaagaa tcgtaaacgc
     3001 ggcgaacttc atcaaaattt tctctcctgt tagagttatc ggttgtaaag catagcgtca
     3061 attcaaaagc aagcgcatgt cttacagcat ccaggacgca aaaccagggt tggtggcttt
     3121 caaagattcg cctgttggaa gaaacatcct gggcggttgt cttctggccg tggggcttct
     3181 gttatctctg ggcgccctcg cgaaaatact gaccggaccc ttagcaggcg gacctccatt
     3241 gattggaatg agcatcctca tcctctcggg gagcatcctg gtcattaccc agatccgccc
     3301 gccaaaattc ctcctattgg acgatgcaaa aggggctttg attatatgcg aaaagacaat
     3361 ggatcagcca actgcactca ttccgtactc cgctttcgag gaaatcagga ccgccagagg
     3421 cctgccttcg cgcaaccgca ggcgaacgtt tggagtggat ctggtcaaaa aagacggggt
     3481 tcgctatgcc atttttgaaa cccggacgct acaggcggcc gaagaagcca ctgccctttt
     3541 gaagaagaac attgacttgc gctctcaaga caacgcttcg cgaatcgtat cgagcacatt
     3601 cagaagggag aacaagaatg atgccatcaa aattgaatgg cgtatgcctg tgcgcgtgtt
     3661 gcgcacgatc ctttctgcgg caatggcaac cgggttttcg atactgctga tagaatcata
     3721 cgactcggtc ccggttctct tctggccttt gcaaatcttt ctgtgcgcga tcgctgtgct
     3781 ttccctggcc tatctctttt atccacgcaa acaggttgtc acaatagatc gcattcaact
     3841 caaagcggaa gtcactggta tctttgcaaa gcaactcatt gttcccctga gcgaaattgc
     3901 gtccgttcaa ttccaatttg cggccgcctg ggaagatacg atttacctgc tgaatgagca
     3961 tgaaactgcg gttatgcgag cgtatcaaga aggaactctt gatccgcagc gcgccctcga
     4021 aatcggaaag atgatgctgc aggccaggag aatcccctct ggaggattga cggcatcaga
     4081 aatgatatca ctcgaacaaa ttctggaaga aaccctcacc gatcagggtc gcgcggtcca
     4141 atgagtttca aggatcactt ttcaaaccat tctagaatct atagtgaatt tcgccccgac
     4201 tacccggatg cgttcatcga atgggtctgc agtctgcctg aaaaaaagaa tctggcctgg
     4261 gactgcggga cgggttccgg acaggccgcg gttcaactca agaaacactt caatcgtgta
     4321 atcgccacgg atccaagcga agagcaaatc aaacatgcta aacaactaga ggggatcgag
     4381 tatcgcgtcc tccccgcaga aaagacaaac ctggatactg cttctgtgga cttaatctgc
     4441 gtagctcaag cccttcactg gtttgatttt gatctgttct tcgcagaagc gagacgagtg
     4501 ctcaagccaa gaggcatcct ttgcgcgtgg agctacggtc tactagaaac agaagaacca
     4561 gttcaaacca tcattgaatc attctacaga gagaccgtgg gaccctactg gccggcggag
     4621 cgacgccacg tagacgaacg ttatgcgacg attccatttc cattttataa gatcgaagcc
     4681 ccatactttc aaatagagag gacgtgggac atggattcat acctgggcta cgtttctacc
     4741 tggtctgcgg ttcaaaggat gcggaaagca actggaatag atccgctgat agaactcaga
     4801 aatcaattgc gtccattcta ccccggtccc ctgctcattc gctggccaat ttttgctctc
     4861 acgggtcgcg caggaaatgc ttgatagaca gtaagggctt tgcgtgcgat tttttttcaa
     4921 ccattctagc cagggctgat gcggagaatg atctggagaa gaaagtagat tgctccagcc
     4981 tttgcggcca gaaagtttaa aaaacgaggc gccacttctg gcgcccacat tgcgtgccga
     5041 atggcaccta ttcgattttt gattatttag caggatttgc gtttttgcaa tctgcttttg
     5101 cactccaggt acccatgctg gttctgtcag catctgttgg tccgaatgtg cctgtgaagc
     5161 tggtgatcag cacgttttcg ttcttagttt cagcgtctgt gtaagtgcct acccagtaga
     5221 acgacttgcc tttgttttcg ccggcagtgt acgtttcgtc aatcttgcag gttttcgcat
     5281 cgcaagttcc agttgtgctg gatttcgcgt ctgcttctgc tgccgcacca gtaatcttcc
     5341 agttgtcgcc ctgtgtgttt gcccaggtta ctttccatgt gaatggaaca gtttcgcctt
     5401 tctcaacata ctgacctttg aagtcacagt tccaggtctc tggagctgga gcgggcttgg
     5461 agcagcttac tacgaacgca gcaactgcaa tgatcaaaat cttcttcatc gtactctcct
     5521 gaatttttcc gtccggttta agaacggacg tcaaggttcc ggcaacgggt gactctgagc
     5581 aactgaaaaa tcaagtcaat ctgatgacga aactaccgct gtgcggcggc agcgttcaga
     5641 atggccatgg caatgagccc attgcagcga cgcgaaggat gcactgcatc ttcccagcga
     5701 ttgcatttct tttcaaagga agtcgaatct gcgacagcca gaccggattc ccggacaatg
     5761 cgatcaaatc cggccgggtc gagttccttg ccgtgtcttt cgtagatggt ctgtcgaaac
     5821 tgtggattta caggtggata ccacactacg gtcggaattc cggaattctt caaaatgccc
     5881 agtaagtgta caaccattgc gtccaggtga tgtctctgcc gcacgggaac cagcgtctgg
     5941 gcgattgaat ctagctgtgc gcgaaacgcc tcgtcatgaa tttcatctga gcccgcgtag
     6001 tctctgacct cgcggccttc gctcacgaag gatctcttct gtccgagcag aagggacatg
     6061 aggtttcttt tagattcaaa ctcgctatca aagcctccga gaatagcagc ctcaggtcga
     6121 aatctgtatc tataagaagg cagggcaatt cgaacgatgg catccagcat catttcgcgc
     6181 ggaaacgatt tcatatgccg caatagaaag tccgtttcgt aaatattctg attgtaggaa
     6241 ctttcaacca gaacctcgtt gaggaactca gggcccagct ccagaacaat gagatccggc
     6301 ttcacacctg aacgcgttag ctcatccgca acaatcaact ctccaagcag gttcgcaccc
     6361 gggaaagcca gccttggatc aaaagtaaac tcgcggagcg tcgcacggct acgatcatcg
     6421 agaagtggat ccgcattcaa gtcatggtgg cgaatctccc cgagcatcat gctacgcgaa
     6481 gacccgatca agagtacatg cttttttcca acctgccggt ccgcatcaag aaccgcgctg
     6541 gcattgtgga cgtaagtctg cagcagctca tgctccgtgg gttctggcct gcccgcattg
     6601 tggacccagg gcaaattcac gggttgatcc agaaccagaa tcaagagcag aagcaagtaa
     6661 ggggtaaacc tcatcttctg ctgatcaagc caatactgac cgaccgcgca atcaaaaatc
     6721 gaaacacaaa aaaagaggag aaataatccg agcacatacg atcttcgaac ctggatcgac
     6781 gataaaacga tccacagtat gacgttctta aaaaaataca cttctctatt tttgaaattc
     6841 ggcctcgtgg gcgcgctggg tacggtcatc aacctggcag tgttctggtt gctcgtggaa
     6901 gtactccgaa tagaaccaaa catagggagt gtggcagcgt tcgtgaccgc actcacagca
     6961 aattactttt tgaatcagaa ctggaccttc agagaggaag ccggaggcga gctattcacc
     7021 gccgggtcgt atgcgcgata tgcgggggta aatctagttg gactcgctgt gaatctaatc
     7081 gtactgaacg ccgccatctg gttgtttcat cctcagggat tgatgctggt tcaggctgca
     7141 gggattgctg ccggaacgat cttcaatttt gtactttcga aactatttgt attcaaggca
     7201 gaacaagatt gaaaatgacg acggagaaaa gacctggtct gtggcaatct ttgtccggtg
     7261 cccatcccgg cgaattgcca gcgctcgaca ttgttcgcgc ggccgccata ctcatggtat
     7321 tcgcctatca cgcatggacc agtacaagaa ccgcggtacc cgcagtggcc caggtagtag
     7381 acaacttgct gttgaatggc aaaagcggtg tgacactatt ctttgttctg agtggctatc
     7441 tgatcacgtc cgttttaatt cgcgaatacg caacatcagc gaacatttcc ctttcgcagt
     7501 tctacctcaa acgcagtctt cgcatcctgc ccgcgtacta tgcttatctt attttcttct
     7561 tgattcttgc agtcatcggc tcgcacacct ttgcaagagg aacagagaat caatttcaga
     7621 gaggttggta tttcgatttt cttttcgttt cgaatatctg gcaagggttt cacgttcaca
     7681 cgtggtctct ggcaaccgaa gaacagttct atttgttgtt ccccgtcctt gcgatcgcgt
     7741 tgtttcgagc cagtaagaga atacgaatcg cgatcattct ggcattgtat cttgttccac
     7801 ttgcaattcg cgtcgttgca ttttcaaaaa tggaacctgc attgtatgga gactgggctt
     7861 atcatcgccc gtggaccaga tttgatgatc tactggcagg aattctggtc gcgtctgcag
     7921 agatctctac tatgcgcgcg atatacaggc gcacgattct ggttgtatgt tccgtggcgg
     7981 ccttatctgt actcgccaca tcgcccagcg aatttacctg gatcagtcct ttcctgaaca
     8041 acggtctaaa tttgagcttc gccgggattg tgctggcggc tcaggcttct cccttaaaaa
     8101 aagcgccggt gttcttcacc gtcacagctc ggttgagtta ttccttctat ttatggcata
     8161 tgctcgcagg cggcataggg gcggcagtgg tgtttcactc cgtcaagcca ggagacactg
     8221 ttacgtggat gaaattcgca agaggaattc tttctgccgc attctggagt tatgtcattg
     8281 ctctggtttc ttatcgcttg atagaaatgc cttttcttcg cttgagagca ccgctcctgc
     8341 tacgaatgca gaagaaatcg agcacttgaa ttcaaccaga catcaagccg atttcgatgg
     8401 ccgcgatcgc caccgaaatc gttcatgaat cagtctcgcg gatcagggag tgacagtcat
     8461 cacgcccatt tgcgccggag agaaactgtt cgttggaaac ggattgtacc cggctccgtc
     8521 gatatcacaa taggtgtagt gtgctcctcc atccatggag aaacggaaca cgtacgcgta
     8581 agaaccagga gccggcgcga tgatcgactt catgtattcg tcatcgttgg ctatttgaac
     8641 attgtaaacc gcagtgacga aagtccacaa accgggttga ttgatgggat cggagcccag
     8701 agggccgtag ccaaaatcag ccagcacgat tggattggga cctgcaacct ctgtgacatt
     8761 ctgtccgtaa actcggccat agacattttg cgttggctga ttggtctgga cggacatagt
     8821 atatggccat tgcagattgc agtattgcaa ctcgttaggc ggccccattc cgtttgcagc
     8881 aacgctcggt ccattgctca gagtgactac gtaatcctgg aaggatccat cttctgcaac
     8941 cacagtatac gtaacgggag ccgcgaaatt gttcggcgtc gatccgctcg tctgaacggt
     9001 ggagccaact tttacggaga ctcctgtcgt tgtgaaactt gcagtgagag cagaaacatt
     9061 cgttcccgca ggcacagtaa tcgcaatgtt cgtgcctgta atcactcctg tagctcccag
     9121 agagggaaaa ctaaaagcag tcaaggcttt cgcattcgag aggatggtca cctgcgcaga
     9181 ggacgtcata cctgccagcg ttgcgtctac agtgccgctc actcctgcag taggcgatgc
     9241 agtgaaaatg ccagaagatg tcccgatggt tccaatgggg ccagtgactc caaaagaagg
     9301 caatacagga atcacattgc cccaggcatc ccgaccgatc gcatcaaatg catatacctg
     9361 gttcactgac agattcagag tcccggtgat cgcaacactg gactgatcca atatcgtaac
     9421 gttcacaagc tgacccggat gcacattgat aggagaggaa aaactaaagg agccagcagt
     9481 gacattcaga tttccgttgc ccacaccttg cgccgtgaag cgaatacccg aaggggaaac
     9541 cttaaccgcg ctgccaacgc caccgcctac attgaacgta tctgcggaga tattctgcgc
     9601 gcctcctaca tcgctcgtta cactcagagt gtagtcgcgt gaaaagccag aagcgatatt
     9661 gagagtggcc gccggattga gagtgatgcc agttgccgtg cgagcaatgg ttcgagtacg
     9721 caccggcgtc gtggtgatct ttccaagatt gtctattgca cggaagtagt actggattcc
     9781 ggttgtggtt actgcactcg ccgggatcag gaatgtatag cgattcggtg tagccgcgtc
     9841 tgttggatca ggcgtgagga cagactgagt gtacgtaggc gcacccactg ttctccagaa
     9901 tacgtacgct gccgcgatgg atccggtact ggttccttga aacgaggtga tgatggtgcg
     9961 aatgcgaagc ggaattcctg ccacaggtgt gcctggaaga aagtttgcgg tggcactgac
    10021 ggaaggtgct gagctgccgg ccacgagcgc ctgcgctcca gcaagataag tcgagaaatg
    10081 atctacagat gcggtgacgc aaccgccgga ttcggaacct cccataccaa tgagaccatc
    10141 ctcatcctgg aggtagactt gcgctgtaga agcggagagg ccttgcgaac tgagctcttg
    10201 agatggatag catatggtga gaagtgcaga ctttgaaaac tgaagtcctt ctggttcgaa
    10261 tttataaccc atgagaaccg gagtaacgcc atccgctgct gcgggcctat cgatcgcggt
    10321 gaccgtaatg tattcatcct ggtcaagagc acctgcaggt actgcaagtt tcacgttgcg
    10381 acccagttcc acatatccgc cttccgatgc agtgattgca ttggatgcat gcgaccctgt
    10441 gctggcccat tcatttgtcg ggatcggaga acctccgtga tagagagcag ccaccccaaa
    10501 cagagtgagc atgtttccgt ttccgtgtga agctggccca acagttcccg tgcaacccac
    10561 ggccgccgta aatagaaccg caatcgccaa attgaataag tttctcataa cacaaatccc
    10621 ccgtctgcga ccagaggggt atacccgggc ctgtgaggtc aaatagatta ttgaaaaaca
    10681 tatacaatca caaacgttat gcgatcaatt cacactttct gtgctgttca ttacacaatt
    10741 catgacgttc attcaggaac catgaagttg atcctcttgg ctcgcggact agaaaacggt
    10801 ccatccacct agaatcccgc agagtggata cctgagcaga agacatcgca atggttcgcg
    10861 ggctgaaatt ccaatcgcct acgatctgtt atgcgatctg gccctaaaag aattggaacc
    10921 atcaacgaaa gaccagggag gccccggaag cacgcgcgaa acttctgatg atttccattc
    10981 aagaggctcg ctccagaatc gcagcattgc acactcgcat gtccattttc gcggttttct
    11041 acgcgctatt ttgagttgcc gcataagctg cctgctgtag tttcgcgaga cgaaaatccg
    11101 ggggacccat gaccattctg catccaacca aattcgattt tagcccgctc gatcctgcat
    11161 cgagcgaaat catgcaaaaa acggttcaat ttttcgaaaa caaagggctc gcaaaggtaa
    11221 aacacgacga ccacgaaagg gtgtggtatg ctgatttcct ggaatttatt aagcaagaga
    11281 agatcttcta tcgcctcatg actcccgcgc aatacgggga ctctccggat gtgcggtttg
    11341 actcttatag aaacaactac ttcaatgaaa tactcgggtt ttacgggctt gcgtactggt
    11401 atacatggc
//
LOCUS       DAOQJL010000051        11384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2883_length_11384_cov_3.863713, whole genome shotgun sequence.
ACCESSION   DAOQJL010000051 DAOQJL010000000
VERSION     DAOQJL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2883_length_11384_cov_3.863713"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..3208)
                     /locus_tag="PLG78_03700"
     CDS             complement(77..3208)
                     /locus_tag="PLG78_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNE22242.1"
                     /translation="MSKRINNIVSRLKGKAVWIAVLIPVFVFPLLPDRTQFVGKVIKD
                     IRYEGLRNVSPDEITGLLTLERGTKVDDATLNADIKALFKSGYFGKISLRGVIEQDGV
                     ILIFEFSELPRVREVDFVGTDKLYAADLKSLVGFKEGEVYAEQKVKSAIPRIKEKYRS
                     EGYFLTEIWYRVSQLDENNEVTVKYIIDEGENIPIGKINIIGARHLDPEDLIGVLEQK
                     EEGTIEDGIFDETKFEQDKFKILGYAKAQGYVDADLDPTGTGYEIRWRSPTKPELGRV
                     VVVTYKLIEGDIRYFGGYSLEFDNDALNEELNPRERPRKNPKPQPVFRKEDLLASMEF
                     SDNDVGDLFDEGKYFRDRTLIQESYSTQGYVFSQVQPGFVNFSLTEETIKKYKNCKTI
                     ANPASDLERKCKKESEWLPLQAMEDHLKKHPEERGRVLRHVHYTVRENNLAYIENIIV
                     KGMVKTQERVIRRELLVKEGHLFNSALVNRSREKIYNLGYFKEVNLQMRPGSDDQKLN
                     LIIDVQEQPTGTISLGGGYGTQSGFSVFTEVGENNLNGTGQKISGKLQYGPLTKQISF
                     QWTDPWIYEACDDSTGSFWRNKQKQFDGAADGESIEKIAASLQNNYSEIGKIIRGYTE
                     SVKSDKSIEALDLTKVKIRRLLRKFVADEEECYRSIPRPWALSLYASYATETIRASSL
                     RISDDSNDLFEGSQYEVSSVGVGAGISHTFWLNWAHYHRYSPQWSIASRPTSLAENEI
                     LRRVGLGWQFKSALTNGLIYDTRDNVFNPTQGLNLDMSVEFVGQMLGGQDHYNQYTIN
                     GKYYMWWFDYTFGGLFRKQALKRWRVVSEFRASATFTHETAPFRGHQNKEINPFIEPQ
                     DKLFLGGYESLRGYDFRTDRNFPLPWQDGGSHMFLAGTELRFPIEPTLVWLAAFLDAG
                     SLYDNVGEFTGDTKTYANNYREIVAVNNARLDAGQLYFLERYSPYNFARYPYDSYYDW
                     NDPLRAVLSERNVSLDRMLFSWGFGVRIQIPVLPLRLFLAQKLYHTSGLKLKPIPGDS
                     KFQFVFGIGDFRF"
     gene            complement(3289..4908)
                     /gene="recN"
                     /locus_tag="PLG78_03705"
     CDS             complement(3289..4908)
                     /gene="recN"
                     /locus_tag="PLG78_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNE22243.1"
                     /translation="MLTELRIENFGIMDEMRFHPGDGLSVLTGETGAGKSLLIQSISA
                     LLGGRLGAGVVRAGAARALIEGRFREKDEDEIVLRREVSADGRSRVFINGTQTNLAGL
                     KARATSLIEMHGQHDQQRLLEPDHQLESLDAYCESSQLRERVASFYHEWQGLSKKLRN
                     VRMQKEERDYRLEYLHHSVKEIDQLSPEEGELERLTQEKALMQNGGKVAQDLHVASLL
                     LGGEEHAVLTGVTRIQQLLDRHDQIIPEFAGSLSDLNEACILLENFHDAIRQQMDRLQ
                     FSPERLEDVEERIQAYRRLIKKHGHSLARVRDDFLKEIHELANVEENEKKLEQNLADS
                     WGRLVEAAELLSRLRRSCIPGLEERMARELSALGMPGAEIRISIHREMSGEEEGTLVI
                     GEKGLDRVEFYFKPNPGEPAHPLRKIASGGELSRIMLACKSVMMEARPVSLVVFDEVD
                     AGVGGEIAHTLAERLRHIARDCQVLVVTHLAQVASRADHHFKVAKKQENGRTVSRIQR
                     LQSEHRVQEVARMMGGARALEFARDCLSMAG"
     gene            5035..5949
                     /locus_tag="PLG78_03710"
     CDS             5035..5949
                     /locus_tag="PLG78_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNE22244.1"
                     /translation="MAVSDRILNRASLGLGILTLCVMILGPLVRANDAGLACPDWPLC
                     YGHVIPPYEFRVYLEFIHRAAVAVMGVLFLAWFVYIMVGFRNQFWKPATLAMALLISQ
                     ILLGALTITEKLDPYIVKSHLINALLYWSCILFVWFRTGPGGSKEARSIKMLSIVLLA
                     LVFGQIYLGGRVSTNEAGLACTSFPACYKIQVFEESGASHWSSIYFPTMMGHVEKHMS
                     HRFMAYALLVFLSVFYWMAGKKMQSRSRSSLRLVLLLTILQSTLGALNVLFQIPVPVT
                     VAHSAIAIVLYGAALIHVLEVTGARQHD"
     gene            5930..6793
                     /gene="cyoE"
                     /locus_tag="PLG78_03715"
     CDS             5930..6793
                     /gene="cyoE"
                     /locus_tag="PLG78_03715"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNE22245.1"
                     /translation="MRGNTIELLKPGIAFSVVITVIPGLLSAGKIPSLALVAATLVGT
                     LLVAMSSFAYNQIIETRTDSMMDRTRNRPLVTGKLPLWQAHALAFVLLVFGTYLLWVQ
                     STPLAAGIALFSFVYYLLIYTLWLKPTTVQSTVLGGVCGAIGPMIGEAAIANRVGMPG
                     IMLFLLLFLWQPPHFWALAIARREDYRKANFPLLPVLKGIPETLRSMIFYQWLLVVAT
                     IAVPLLGLAGWIFTVPSLFSSLVTLLLIYRMRKDASASSAMKVFFASIAHNLVWHAAF
                     SVDAAVGILRD"
     gene            7098..7502
                     /gene="speD"
                     /locus_tag="PLG78_03720"
     CDS             7098..7502
                     /gene="speD"
                     /locus_tag="PLG78_03720"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNE22246.1"
                     /translation="MEDPLGQHYIIELYECNHSVLDDLKSVRDTLMEAARIAGATIIG
                     EKFHRFSPQGVSGVVVIAESHLSIHTWPELGYAALDLFTCSHGMDIQAALDLLKRSFQ
                     PQDMDVHFMKRGVLKKSRSHAPELFDFVPAAV"
     gene            7517..8422
                     /locus_tag="PLG78_03725"
     CDS             7517..8422
                     /locus_tag="PLG78_03725"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNE22247.1"
                     /translation="MITTKIHNTIQSYLNSKVRRMRGLAGDGLFAARAIKKDELVCMW
                     GGTIYTGEQFEEQPEAMKHYGLQVGENLYIAPANLDSVDDAEYFNHSCEPNCGFRGQA
                     ALVAMRDIRAGEELTFDYAMAETYDHPWKCECGSKHCRGTLRGTDHNLPELRDRYRGY
                     FSEWLEKKHADQLDDADYLHRFRATGAWGLDTGLDLHECNPEIIRSAEKIREFTIKLC
                     EKIEVKRFGEPVIVHFGEDERVAGYSLVQLIETSLISAHFANQTNRVYLDVFSCAYYN
                     PTEVAEFAKEFFQAKSVNVQTQLRH"
     gene            complement(8515..9765)
                     /locus_tag="PLG78_03730"
     CDS             complement(8515..9765)
                     /locus_tag="PLG78_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE22248.1"
                     /translation="MELSKEQRKVVSANDRFLQVVAAAGSGKTRTMIALAEDSVTRGM
                     NPARILMLSFSRKACSEIRSRLSESIRSEVRVSTFHSLAFRSMMRRREMRGVRVLTDQ
                     AKFEFMRGLLCQAKTDGIPYSVLLANKRLFREQFPDLCMSVYRELAAYKRSNRLAEFD
                     DMVLDLNRELRLGHLRDLRKSFDLIIVDEFQDTDPGQLQFLQMMDSRRLVVVGDDYQS
                     IYGFRGSDPELFLKFQKHFPGARVLSLSENYRSQERIVRAGNHLIRSSRKQLRKKVRA
                     MRRGSTDCVALSVFRGEEAEVAALVNASGGFILCRSNYRRIVWERAGVEPDRLLTIHS
                     AKGLEFPIVYLDLCGGWSSARGTRVPDEEVRVAYVGLSRAENWLVVMHDESGTSAPER
                     AVFELFRNEVQRYSPVALQRAMAA"
     gene            9859..10206
                     /locus_tag="PLG78_03735"
     CDS             9859..10206
                     /locus_tag="PLG78_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE22249.1"
                     /translation="MNNLALAVLDGNLTRDPETKTIKTGKITTTFTVAMNHEYGNKDG
                     NKSVSYIQVETWDKLAENCANFLKKGSRVTVSGSLREDRWKTEDGKARNRHKVVAQTV
                     RFDSPKKDQEEAA"
     gene            complement(10267..>11384)
                     /locus_tag="PLG78_03740"
     CDS             complement(10267..>11384)
                     /locus_tag="PLG78_03740"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNE22250.1"
                     /translation="FLDGLTRIKAGLKSKRVRIKGPDPWMAYVEGLLSRSDHRVGQYI
                     LRAYKEGACFDSWDDRFQKDTWKKIIAEIPADLVSLWMDQKPGGSRVPWDEIIDGALK
                     EKLVRDFEKFESITPETMNPARVQELKPSDFPPELLKPVTIPEEKFKTAGFLNLSFAK
                     SGAFLYVSHLDTMNAMRKVLRRAKIPMTFSVGFNKHERLHFQGGTPTYFHSDAERLSA
                     ETYARFDLVAAEKRIRENLPMGLDLLELSFTDRKGFSEAAWHSFRVDFFTESKFEPVR
                     ARLNDLPEEISFEKRDRKKKHGPRHANFMKKVKKRVRTCVRAVEAHEAADGMAWIKFE
                     LESTERGGGISVTDLLSRVLDLPVETWNVDVRLTRLS"
BASE COUNT         2729 a         3041 c         2724 g         2890 t
ORIGIN      
        1 aacaatccgc gtcttatagc gaaacaaata gcgaaacagc ctcagcgtga tgctgaagcc
       61 aggcgaagca ggtgggttag aatcggaagt cgccaatgcc aaacacaaac tgaaatttgg
      121 aatcacccgg aatcggcttc agcttcaacc cagaagtgtg atagagcttc tgagccagga
      181 acaatcgcag aggcagaacc ggaatttgaa tcctgactcc aaaaccccat gagaacaaca
      241 ttctgtcgag cgatacattt cgctccgaca gaacggcacg aagcgggtcg ttccagtcat
      301 aatacgaatc atacggatac cgggcgaagt tgtagggact gtagcgttcc aggaaataca
      361 gttgtcccgc gtcaagcctc gcattgttta cggcgacaat ctcacgatag ttatttgcat
      421 aagttttggt atcacccgtg aactcgccca cgttatcgta aaggctgccc gcatccagaa
      481 atgccgcgag ccacacaaga gttggctcaa tcgggaaacg tagctccgtt cctgccagga
      541 acatgtggga gccaccgtcc tgccatggta gagggaaatt cctgtccgtg cggaaatcgt
      601 atccgcgcag cgattcatat cctccgagga acaacttgtc ctgcggttca atgaatggat
      661 tgatctcctt attctgatgc cctctaaacg gcgcggtctc atgcgtgaat gtggcagatg
      721 cgcgaaactc agaaaccacg cgccatctct tcaacgcctg cttgcgaaac agtccgccga
      781 acgtatagtc aaaccaccac atgtagtact taccgttgat ggtatactga ttgtagtgat
      841 cctggccacc cagcatctgt ccgacaaatt ctactgacat atccagattc aagccctggg
      901 taggattgaa gacgttatca cgcgtatcgt aaatcagacc gtttgtaaga gctgacttga
      961 actgccagcc aagcccgact cttctgagaa tttcgttttc cgctagagaa gttgggcgcg
     1021 atgcaatgga ccactgcggc gaataacggt ggtaatgcgc ccagttcagc cagaacgtat
     1081 gcgaaatacc tgcgcccact cccacagatg atacttcgta ctgggatcct tcaaaaagat
     1141 cattggaatc gtcgctaatg cgcaaagaag aggcgcgaat tgtctccgtc gcataactgg
     1201 cgtaaagcga aagagcccat ggccttggaa tacttctgta acattcctct tcatcggcga
     1261 caaacttgcg aagcaatctc cggatcttca ccttggtcag gtcgagagct tcaatcgatt
     1321 tgtccgattt tacggactct gtgtacccgc ggatgatctt accgatttcg ctgtagttgt
     1381 tctgaaggct cgctgctatc ttttcaatgg actcaccgtc ggcggctcca tcaaattgct
     1441 tttgcttatt tctccagaag cttccggtcg aatcgtcgca tgcttcgtaa atccagggat
     1501 ctgtccactg aaagctaatc tgcttggtga gcggaccgta ttgcagtttg cctgaaattt
     1561 tctggcctgt tccgttcagg ttgttttcgc ctacttctgt gaagacggag aatccagatt
     1621 gtgttccgta tccaccgccg agcgaaatgg ttccggttgg ttgctcctgc acgtcaatga
     1681 tcaggttcag tttttgatca tccgagccag gtcgcatttg caggtttact tctttgaagt
     1741 aacccaggtt gtagattttt tcgcggctcc gattcaccag agcagagttg aaaagatgcc
     1801 cttctttcac aagaagctca cggcgaatca ctctctcctg ggttttgacc atacccttta
     1861 cgatgatgtt ttcaatgtag gctagattat tttcgcgcac tgtgtagtgc acatggcgaa
     1921 gtacacgacc acgctcctca ggatgtttct tgagatgatc ttccattgcc tgcagaggaa
     1981 gccactctga ctctttcttg cactttctct caagatcgct tgccggattt gcaatggtct
     2041 tgcaattctt gtatttcttt atggtttctt ctgtgagaga gaaattaacg aatccgggtt
     2101 gcacctggga gaatacataa ccctgagtgg agtatgattc ctggatcaaa gtgcggtcac
     2161 ggaaatattt tccttcatca aatagatccc cgacgtcatt gtcagagaat tccatggacg
     2221 caaggaggtc ttccttgcga aacacaggct gaggttttgg gtttttgcga ggtctttcgc
     2281 gagggttgag ttcttcattg agcgcatcat tgtcaaactc aagggagtaa cctccgaagt
     2341 agcgaatgtc tccctcaatt agtttgtacg ttacaactac cacacggccg agttctggtt
     2401 tggtagggga tctccaacgg atttcgtaac ctgttccagt tggatccagg tccgcgtcta
     2461 cgtaaccctg agcttttgca tacccgagga ttttgaattt gtcctgctca aacttggttt
     2521 cgtcgaaaat gccgtcttca atggtccctt cttctttttg ctcaagcact ccaatgagat
     2581 cttccggatc aaggtgacgg gccccaatga tgttgatctt gccaatggga atgttttccc
     2641 cttcgtctat aatgtattta acggtgactt cgttgttttc gtccagctga gagacacggt
     2701 accaaatctc cgtgaggaaa tagccttcgg aacggtactt ttccttgatg cgggggatcg
     2761 cagacttcac cttctgctct gcatagactt ctccctcttt aaaccccaca agacttttga
     2821 gatcagccgc atatagcttg tctgtgccga cgaaatcgac ttctcgtact cgcggcaatt
     2881 cgctgaattc gaaaatcaga atcacgccat cctgttcgat gacaccgcgc agggatattt
     2941 ttccgaaata tcccgacttg aacagagcct ttatgtctgc attgagagtt gcatcgtcta
     3001 cttttgtgcc tcgctccaga gtgagcaacc ctgtgatttc atccggagag acgttgcgca
     3061 aaccctcata gcgaatgtcc ttgatgacct tccctacgaa ctgggtgcga tccggcagaa
     3121 gagggaagac aaagacagga atcagaactg cgatccagac tgccttgccc ttcaggcggg
     3181 atacgatgtt gttgattctt ttgctcaatc ccgatttgac ctgacgggac ccggaggatt
     3241 ccgcgtcccg gccagaaaat atttgggaag agatgcgtca ttacaattct aaccggccat
     3301 agaaaggcaa tctcgagcaa attccagggc tcgcgcgccg cccatcatcc ttgccacctc
     3361 ttgcactcta tgctccgatt gtagtctctg aatgcgagac acggttcgcc cgttctcctg
     3421 cttcttggcc accttgaaat gatgatccgc cctcgaggct acctgggcca gatgagtgac
     3481 tacaagaacc tggcagtcgc gtgctatgtg tctgagtctt tctgcaagag tgtgcgctat
     3541 ttcaccaccc actcccgcat ccacttcatc aaacaccacc agcgatacgg gacgcgcctc
     3601 catcatgacg gatttgcacg caagcataat cctcgaaagt tccccgccgc ttgcgatctt
     3661 acggagtggg tgcgcgggtt ctccaggatt tggtttgaag taaaactcca cccgatcgag
     3721 gcccttctcc ccgatgacga gcgtaccctc ttcttccccg ctcatttccc gatggatgga
     3781 aatgcgaatt tctgcgccag gcattccaag tgcagacagc tctcgcgcca tacgctcttc
     3841 cagcccagga atgcaggatc tgcgaagcct ggagagtaat tctgcggctt cgaccaatct
     3901 gccccaggaa tccgccagat tttgctccag tttcttttca ttttcttcta cgttcgcaag
     3961 ctcgtgaatc tctttgagaa aatcatccct gactctggcc agcgaatgcc catgcttctt
     4021 gatcaagcgt ctataggcct gaatcctttc ctccacatcc tccaacctct cgggagaaaa
     4081 ttgcaggcga tccatttgct gacgaatcgc atcatgaaaa ttctcaagca gtatgcacgc
     4141 ctcgttcaaa tcggaaagag aaccggcgaa ctccgggatg atttgatcgt gacgatcgag
     4201 gagttgctga atgcgagtga ctcccgtaag gactgcatgt tcttctcctc caaggagcaa
     4261 agaggcgacg tgtagatcct gcgccacctt ccctccgttc tgcatcaaag ccttttcctg
     4321 cgtgagcctc tccagttccc cttcttccgg ggaaagctga tcaatttcct tcacagagtg
     4381 atggaggtat tccagcctgt agtctctttc ttctttttgc atccgcacgt ttcgcagctt
     4441 cttcgaaaga ccctgccact cgtggtagaa cgatgccact cgctcgcgca gttgagaaga
     4501 ttcgcagtac gcatccagag actccagttg atgatccggt tccaatagtc tctgctggtc
     4561 atgctgtccg tgcatttcaa tgagagaagt agcacgtgct tttagacctg ccaggttggt
     4621 ctgtgttccg ttgatgaaga cgcggctcct tccatccgcg gacacttccc ttctcaatac
     4681 gatctcatct tcatcctttt cccggaagcg accctcaatc aacgcccgag ccgcacccgc
     4741 gcgcactaca cccgcaccga gtctgccccc caggagtgca gagatagatt gaatcaaaag
     4801 cgatttgccc gcacctgttt cccctgtgag tacggacagc ccatcacccg gatgaaatct
     4861 catttcatcc atgataccga agttttcaat gcgtagttca gtcagcatgg ggacaggcta
     4921 atatgtaaac atatgttagc caatcaaaat ttggtactct tgtgtatttt ttcgagtcga
     4981 gagggagtcg caaattgatt tgtaggggtg ggtccccgca aaaacctgga atgaatggca
     5041 gtttccgatc gaatcttgaa ccgggcatcc ctggggctgg gaatcctcac cctctgcgtg
     5101 atgatcctgg gtccgctggt gcgagccaat gatgccggac ttgcctgccc ggactggcct
     5161 ctctgctacg gccatgtgat ccctccttac gaatttagag tctacctgga attcatccac
     5221 agggccgcag tcgccgtgat gggtgtgctc ttccttgcct ggtttgtgta tatcatggtg
     5281 ggattcagga atcaattctg gaaaccggcg acactcgcca tggcgcttct catttcccag
     5341 attctgctcg gtgctctgac cattacagaa aagctagatc catacattgt caagagccac
     5401 ctgatcaacg cacttctcta ctggagctgc atcctttttg tgtggtttcg cacaggcccg
     5461 ggcggaagca aagaagctcg ttctatcaaa atgctttcta tcgttttgct cgcacttgtg
     5521 ttcggacaaa tctatctggg cggaagggtc agcacaaacg aagcaggact cgcttgcaca
     5581 agcttccccg cgtgttataa aatccaggta tttgaagaga gcggtgcgag ccactggtcg
     5641 agcatttact ttccaaccat gatgggacat gtggagaaac acatgagcca caggttcatg
     5701 gcttacgcac ttctagtctt cctgtccgtt ttctactgga tggcagggaa gaaaatgcag
     5761 agtcgatcca gaagcagcct cagactggtt ttgctgctta caattctaca atccacgctg
     5821 ggcgctctca acgttctatt tcagattccg gttcctgtca cagtggccca ttctgccata
     5881 gcgattgttt tgtatggcgc ggcgctgatt catgtgctgg aggtcaccgg tgcgcggcaa
     5941 cacgattgag ctgctgaaac cgggaatcgc tttctcggtc gtcatcactg taattcccgg
     6001 cctcttgtcc gcgggaaaaa tcccatctct ggctctcgtc gccgccactc tagtcggaac
     6061 tctgctcgtt gccatgtctt cctttgcgta caaccagatc atcgaaacga gaacagactc
     6121 gatgatggat agaaccagaa atcggccact cgtgacaggc aaacttcccc tctggcaggc
     6181 gcacgcgctc gcattcgtct tgcttgtatt tggaacctat ctgctctggg ttcagtccac
     6241 tccactcgct gcgggaattg ctcttttctc ttttgtatat tacctcttga tttacactct
     6301 ctggctcaag ccaacgacag tgcagagcac agttctagga ggagtgtgcg gcgcgatcgg
     6361 tccaatgatt ggggaagccg ccattgcaaa tcgcgtcgga atgcccggaa ttatgctgtt
     6421 tttgctactt tttttgtggc agcctccgca cttctgggcg cttgcgattg cgagaagaga
     6481 agactatcgc aaggcaaact ttccgctcct gccagtgctc aaagggattc cagaaactct
     6541 tcgaagcatg atcttttacc agtggcttct cgtagtagcc acaattgctg ttccacttct
     6601 cggactcgca ggctggattt tcacagttcc atctctcttc tcctccctgg tgaccctgct
     6661 tttgatttac agaatgcgca aagacgcctc ggcaagctct gccatgaaag tttttttcgc
     6721 ttcgatcgct cacaacctcg tctggcatgc tgctttctct gtggatgcgg ctgtgggaat
     6781 tctcagagat tgaacactgc agtacgcgtg ccttatgtcg gatttcggca aaaaaatcgc
     6841 cgtttcacgg ctattttcgg ggtttcttgt ctgggacata atcccgcgaa gtcatttcat
     6901 ccgagtcgag gaagatttgc gcgggtgaaa ttcgcgctgt cgcaaaatct gaaaaatttt
     6961 tcccgaagat ttttttttaa tttgcgtaaa aaatgacgat aggtactcac gtgcaaacgt
     7021 caaacgaata gtttgatcgg ggttttctaa atagttgcaa tcctacaagt atctagtagg
     7081 agcagcggga ggtgaggttg gaggatccgc ttggccaaca ttatatcatc gagttgtatg
     7141 agtgcaatca ctcagtactg gatgatctca agtctgtcag agacactctg atggaggcgg
     7201 cccggatagc cggtgccact atcattggcg agaagttcca tcggtttagc ccgcagggcg
     7261 tatccggtgt tgtcgtaatc gccgaatccc acctgagcat acatacctgg cctgagcttg
     7321 gttatgctgc tctagatctt ttcacctgta gccatggcat ggacattcag gcggcactgg
     7381 atttgctgaa acgcagcttc caaccgcagg atatggacgt tcatttcatg aagcgcggtg
     7441 ttttgaaaaa atccagaagc catgcaccgg aattgtttga tttcgttccg gcagcggttt
     7501 gaaggaggga agcaggttga tcacaactaa gatccacaac acaatccaat cctacctcaa
     7561 ctcgaaggtt cgcagaatgc gcggccttgc gggggacggt ctatttgcag cgcgcgccat
     7621 caaaaaagat gagctcgtat gcatgtgggg cggaacaatc tacacgggcg aacagttcga
     7681 agaacagcca gaagccatga aacactatgg ccttcaggtg ggcgagaatc tgtatatcgc
     7741 gccggccaac ctcgattccg tggacgacgc ggaatatttc aaccacagct gtgaaccaaa
     7801 ctgcggcttt cgcggtcagg cggctcttgt ggctatgcgg gacatccgtg cgggagaaga
     7861 gctcacgttc gactacgcca tggcggaaac gtatgaccac ccgtggaagt gcgaatgcgg
     7921 cagcaaacat tgccgtggca ccctgcgtgg aacagaccac aacctgccgg agcttcggga
     7981 ccgttatcgc ggttacttca gcgaatggtt ggagaaaaag catgcagacc agctggacga
     8041 tgcagactac ctgcaccgct tccgggcaac gggtgcatgg gggctcgaca caggtctcga
     8101 cctgcatgaa tgcaatccgg aaatcatccg tagcgcagaa aagatccgcg agtttacaat
     8161 caaactctgc gaaaagatcg aagtgaagcg ctttggagaa ccggtcatcg tgcacttcgg
     8221 cgaagatgaa cgagttgccg gctattctct ggtgcaattg atcgagactt cattgatctc
     8281 cgctcacttt gcaaaccaga ccaatcgcgt gtatctggat gtattcagct gcgcttacta
     8341 caacccgacc gaggttgcag aattcgcaaa agaattcttc caggcaaaat ccgtaaacgt
     8401 ccagacgcag ctcagacatt gatctgagct ctgctcctgg caagaaaccc cggccaatgg
     8461 tcggggtttt ttttatgcct ttgttcaaag ccgtatctgg cctaaaagct accgtcaagc
     8521 ggccatcgct ctttgcagcg cgacgggcga atacctctgc acttcatttc gaaagagttc
     8581 aaacaccgct ctttctggag cggatgttcc agactcgtcg tgcatcacaa ccagccagtt
     8641 ttcagcgcga ctcaaaccaa cataggcgac cctcacctct tcgtccggca cacgagttcc
     8701 tctagcagaa ctccaaccgc cacaaagatc cagatataca attggaaact caagaccctt
     8761 ggcagaatga atcgtaagca atcggtccgg ctccactccc gccctctccc agacaattct
     8821 tctgtagttg cttctgcaaa ggatgaagcc gccggatgca ttcacaagag ctgccacctc
     8881 cgcttcttct cctctgaata cggaaagggc cacgcaatct gtagatcctc tgcgcatggc
     8941 gcgcaccttc tttctcaatt gtttgcgcga tgaccggatc aaatgattgc ctgcgcgcac
     9001 gattctttcc tgactcctgt aattctcgct caaagaaaga actcgcgctc ccgggaaatg
     9061 cttctgaaat tttaggaata attcaggatc gcttcctcga aagccgtaga tgctttgata
     9121 atcgtctccc actacaacga gtctccgcga atccatcatc tggagaaatt gaagctgccc
     9181 gggatctgtg tcctgaaatt cgtctacgat gatgagatca aacgatttgc gcagatcgcg
     9241 cagatgtccc agacgaagct cgcggttcaa gtccagaacc atgtcgtcaa actccgccag
     9301 gcgattggat cttttgtatg ccgcaagctc tctataaaca ctcatgcaaa gatccggaaa
     9361 ctgctctcta aatagcctct tattggcaag caggaccgag tagggaattc cgtctgtttt
     9421 agcctggcag aggagcccgc gcatgaattc aaacttcgcc tgatccgtca gaactcgcac
     9481 gccgcgcatc tctctccttc tcatcataga acgaaaggca agagaatgaa atgtggaaac
     9541 gcgcacttcg cttcgtatgg attctgaaag cctggagcga atttcagagc atgcctttct
     9601 actaaaactg agcatcaaga tgcgcgccgg attcatccct ctggtaactg agtcttccgc
     9661 cagagcgatc atggtgcgag tcttgccgct gcccgcggcc gccaccacct ggaggaaacg
     9721 atcattggca ctcactactt tccgttgttc tttcgatagt tccatcctat cactgggtaa
     9781 tcgaaaaaga aaatgatccg aacaatttgt gcggaacgat caaaagcaca gttacccttc
     9841 ccttgcaagg agacacttat gaacaatctg gcacttgcag tgcttgatgg aaacctcacc
     9901 agggatccgg agacaaagac aatcaaaacg ggaaaaatca ccaccacctt tacggtggcg
     9961 atgaaccacg aatatggaaa caaagacgga aacaaatccg tctcctacat tcaggtggaa
    10021 acctgggaca agctcgcaga aaactgcgcc aactttctaa agaaaggcag cagagtgact
    10081 gtgagtggtt ctttacgaga ggaccgctgg aaaacagaag atggcaaagc gcgcaaccgc
    10141 cataaggtgg tagcacagac cgtccgattc gacagtccaa aaaaggatca agaagaagcc
    10201 gcttgagtta gccgggccag gcctctgaaa gacgatacgt cacaacgcct gcaggcgtgc
    10261 gccgtctcag gagagcctgg tcaatctcac atctacgttc caggtttcca ctggaagatc
    10321 gaggacccgc gacaggagat ctgtgacgga aattccgcct cctctctccg tggactccag
    10381 ttcaaacttg atccaggcca ttccatctgc tgcttcgtgc gcttccacag cacgcacaca
    10441 ggtgcgtact ctcttcttaa ccttcttcat gaaattcgca tgacgtggtc cgtgcttctt
    10501 cttgcgatct ctcttctcaa atgaaatctc ttccggcaga tcattcaatc gcgctcgcac
    10561 cggctcaaat ttggattctg taaagaaatc tacgcggaag gaatgccagg ctgcctctga
    10621 aaatcccttc ctgtctgtga aagacaactc gagcagatcc agacccatgg gcaggttttc
    10681 tcgaattctc ttttccgcag ccaccagatc aaaacgagca taagtttccg cagaaagcct
    10741 ttctgcatct gaatggaagt aagttggcgt tccaccctga aaatgaagcc tttcgtgctt
    10801 gttgaaccca acggagaaag tcataggaat cttcgcacgg cgaagcacct ttcgcatggc
    10861 attcatcgtg tccaggtgac tgacgtagag aaacgcaccc gatttggcaa aagacagatt
    10921 gagaaagcca gctgtcttga acttctcttc tggaatggtg acaggtttga ggagctccgg
    10981 aggaaaatcg gagggcttga gttcctgcac tcgcgcagga ttcattgttt ccggagtgat
    11041 ggactcaaat ttttcaaaat cgcgaaccag tttttctttc agagccccgt caatgatctc
    11101 atcccaggga actcttgatc cgcctggctt ctggtccatc cacaaagaaa caagatctgc
    11161 tggaatttcc gcgatgatct tcttccaggt atctttctga aaacgatcgt cccaggaatc
    11221 aaaacaagca ccttctttgt aagcacgcaa aatgtactga cccactcgat gatcggatct
    11281 ggacagaagt ccttccacgt atgccatcca gggatctggt cccttgattc gaactctctt
    11341 tgatttaaga cctgctttga ttcgagtgag accatcgaga aaat
//
LOCUS       DAOQJL010000052        11338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2902_length_11338_cov_4.449526, whole genome shotgun sequence.
ACCESSION   DAOQJL010000052 DAOQJL010000000
VERSION     DAOQJL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_11338_cov_4.449526"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PLG78_03745"
     CDS             <1..934
                     /locus_tag="PLG78_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNE22251.1"
                     /translation="HTGEGVSGTEVKSLFAFIFCLGAFPLHAIVPVPPLQGRVQDLTE
                     TLTAAEKTALEQKLSDFEKTKGSQVVVLIVPSTEEEAIEQFSIRVAEAWKIGRKGVDD
                     GVILLVAKSDKKLRIEVGYGLEGALPDAICKRIIDEQIVPRFRAGKFGEGITAGVDSI
                     LQRINGEPLPAPFVSNSQDLTGIPWTMQLIVTAFLSIFIIVGSLVPSAVNKFLCILPV
                     AAGLAAAVAGYVVGGHWIVAVMFAAFATFFVGVFTTVMSGFFLSIFGLITGKKVGGWR
                     ISTSSGSSGWSSSSGSSFSGGGGSFGGGGASGSW"
     gene            984..1439
                     /locus_tag="PLG78_03750"
     CDS             984..1439
                     /locus_tag="PLG78_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22252.1"
                     /translation="MATSTEKKKQTDRDLWRELTTRKEEFSKIVKLLVEFDEKTGRYL
                     KYNHPETHYMRRAVAACDPENLRIFLKSLGGFVYSVVAEVKSAAGLTATAWVHEDGIR
                     EEREGCDTGHPVHQIVCVTDLFEADSQTIDVVHSCVSQPAADLLAERGE"
     gene            1484..2227
                     /locus_tag="PLG78_03755"
     CDS             1484..2227
                     /locus_tag="PLG78_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22253.1"
                     /translation="MIEITGLKKSFKGMPVLTGLDLTIQDDETMAVIGPSGCGKSVLL
                     KHLVGLLRPDEGKIIIDGEDITRYSDSKLYDLREKFGMLFQSAALFDSLSVGENVGLW
                     LKEHTKTPETEIAVKADRILNLVGLRDVMEKRPSELSGGMKKRVGLARAIINEPKYVL
                     YDEPTTGLDPIMSDIINDLIIKVRMELKNTAIVVTHDMASVYKVADRIAMMHQGKIVF
                     LGKPDDIKTTDNPLAQQFIQGKAQGPIRL"
     gene            2274..3065
                     /locus_tag="PLG78_03760"
     CDS             2274..3065
                     /locus_tag="PLG78_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22254.1"
                     /translation="MHQTMIELKAVKKRFGAREILRGVDIDIRRGETMVLLGGSGTGK
                     SVTIRHIIGLLEPDEGEVRVDGHLMSGATKNTKTKLRARMGVLFQSGALINWLTVYEN
                     VALPLRETSKKTPKEIDEIVREKIAWLGLTPAMNLLPDKISGGMKKRVGVARALTTQP
                     EIILYDEPTSGLDPVMSQVLNELNNRLRDELKVTQVVVTHDMNSAFTIADRITFLYQG
                     KVRCVGTPRDIQESTDPVVQQFIKGETVGPMQIDAGDPTAKKADS"
     gene            complement(3062..5179)
                     /locus_tag="PLG78_03765"
     CDS             complement(3062..5179)
                     /locus_tag="PLG78_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNE22255.1"
                     /translation="MSLRVRFYGGLTILAVITSLTALYHLYEIRSIRAELQEVQEDAI
                     PSVVLADRMVVATVEVQQWLTDVSVTGDRSGYSDAETARTEFKTALAEFRKLRGNEKL
                     MDFDALQKEFDRYYDLGSEMTEAYLTQGQAGGNRSMRRFDAQADSIAERVGKLRTQSL
                     SHVDASSADIKKSVSLAWYSNIIQLIITLILILAAAIGMYRGFIVPVYALRDSLRTAL
                     QENDLGARIPAGNAEEMRQIASWVNAFIESVSFLVLQVKEQSVLVQSQTNDLERSSSR
                     LSTEAEHLAASTEEGSAALEELAAAAENVGERAERLALVSKRIEENVRNLGKSIHEIS
                     RNMDQARSISNEALTRAAAGRRSMDEGLVTMEHVQERAERIHEVVGVINGISERTSLL
                     ALNASIEAARAGDLGRGFAVVASEISKLADHAATSAEEIARLIEETSTEMKSSSESVR
                     GVLGILTGLEDGTRQMAAAFEQIGESLKSQDRSAVEITKEASEIVDSSGDIDQAMREQ
                     RKTTQEISAGVETSSNSSRILAEDADRLNRIVGQIRNLMLMAERVLSRFKMPEVGRVL
                     QWSESYSVGVPQMDAEHAKIFEIMNQLTWMVKEGGSDAEQMRHLMDDLSEYCKFHLSS
                     EEQFMHDHRYPALDEHMQLHADFVKTVEGYGARIHDGEDLRFLAFEMCGTIWLWLTNH
                     ILVEDRKYGTFINSRRALQSGPR"
     gene            5272..6717
                     /locus_tag="PLG78_03770"
     CDS             5272..6717
                     /locus_tag="PLG78_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22256.1"
                     /translation="MRIRRFVLPVFVALICAILVYVYMKTGHGTSRAFRNICFVEFFA
                     ATLIATVSPRWRFVFAGFATLTLSRLPSLLLQIPTHNLLFPIFFGLALGAYFRAARND
                     LSEPVARFGVAQILAVAIIIGCVRAILQNYWEPGIGLVLENRILSDQMTSNYAVFLAL
                     TLGLHLLAPIVWICVEQVNPELEAGDMASRLSIGILWGIGANLIVMLLQGFVSPLNTG
                     ITDRAIEAGRRAGLFSDSGSAGAAFLAFVLPASFAFASLFHARIKKNKRWLASLLFFW
                     AIAIFTASFFGRVIVLASVGALVAWLFLIPLNRRVRLIAGFAILFSFVCFGLFLIWSG
                     PVAFLSRIDPVRAQLLETGIVLIRQSPWLGHGFNSFVPALMELRKSDPTILIENPAGL
                     IPGILNDAGIVGLCILVAMLVYYVLGLRLLFLRKGADENAAEATVVVILSTLSMFLVF
                     TVGYHILAAEVAAFVFFPLLVLPDSFRRTKS"
     gene            complement(6891..7457)
                     /locus_tag="PLG78_03775"
     CDS             complement(6891..7457)
                     /locus_tag="PLG78_03775"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNE22257.1"
                     /translation="MKIYTKQGDAGWTRLANGEKTRKSDVRVDLYGTADELNSHIGLA
                     LSQPGVPEETRAQLLDLQHLLFELGSELAGYYKNKEGSIIREEDTGKLEQWMDQCTAV
                     LPPLRAFILPGGEAAASALHICRTVCRRLERAMTAALDENSGERPVVYPEAYKFINRL
                     SDYLFIAARYVNFRAGKSDIEWQTRETK"
     gene            7544..8362
                     /gene="larE"
                     /locus_tag="PLG78_03780"
     CDS             7544..8362
                     /gene="larE"
                     /locus_tag="PLG78_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNE22258.1"
                     /translation="MPVSSELMARRERLIHLLSPHTVPGLIVAFSGGVDSACLLWAAR
                     EAAKKSAGRVLAITAVSASVPDLDLRDASEFAEKLGVEHRILRSRETENQEYLKNDSN
                     RCFHCKTELFRIAREEADAEGIQHIAYGYTISDRGDVRPGHRAARMAGVLAPLEEAEL
                     TKEDIRAILREEGFSLSEKPAAPCLSSRIPTGLPVTIKRLEDVQQVETFLRDHGVQIV
                     RARIHDEKTIRIETEDASAAKVLELRHDLERLSRSLGYRWITLDLGGYRTGGST"
     gene            8359..9162
                     /gene="larB"
                     /locus_tag="PLG78_03785"
     CDS             8359..9162
                     /gene="larB"
                     /locus_tag="PLG78_03785"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNE22259.1"
                     /translation="MNLNDVRKLLERLISGEENVDGVIRQLRAGPLRMERTADGFPDH
                     HRLLRTGMAEAIFGESKSIEQITSLARSLSENGAPVLITRLDAAKRSALLALFSGSRV
                     SDQAKTILIHPPEILEVQAVDAPSYAEPFAAIVTAGTSDIAVAEEAVEVCIACSIPFL
                     RIRDAGVSGLHRILSYAEALQNASAIVVCAGMEGALPGVVAGMTGRPILAVPTSVGYG
                     ANLGGFSALLTMVNSCAAGVSVVNIDNGFSAGYAAASVIREIHRFQRKN"
     gene            complement(9377..10777)
                     /locus_tag="PLG78_03790"
     CDS             complement(9377..10777)
                     /locus_tag="PLG78_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNE22260.1"
                     /translation="MAKHRLVASPQLMISVSGIRGILPTGLSPSVIVDAVLGFGTVTG
                     KKIVIGMDSRNSGPLIRDLVCATLRAQGKEVLDAGLAPTPTVKAAVKLEHAAGGIMIS
                     ASHNPPEWNGLKLMGPEGFFFNKFNLSSMMTALESGKLTGADHKTLGSFHEIDAVSKH
                     VKTVLNLLNNVEQIRAKKYKVVVDAVGGAGRNALPELLTELGCEVIPLFCEAASGFDF
                     PRPPEPTPVALKKFSQLLKKESAAVGFALDPDADRIVPGSPGRGAINEEYSLPLSFLG
                     LPEIKDVPKSQPRGKTPFVVNLSTSTLLDQVAAPYGYPVIRSAVGEANVVEMMKKNKS
                     FFGGEGNGGIIHSRVPSFGRDSLLGAALILSAMARKNAATLDDVLNHLPELFMEKRKF
                     DVKAGTDVRELFSRIEGLFPGMRADRKDGLHLSDAEGWIHARSSNTEPVIRIIAEARN
                     KKHLEELLSRAARVVK"
     gene            10847..10929
                     /locus_tag="PLG78_03795"
     tRNA            10847..10929
                     /locus_tag="PLG78_03795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10881..10883,aa:Leu,seq:gag)
BASE COUNT         2579 a         2788 c         3003 g         2968 t
ORIGIN      
        1 acatactgga gaaggtgttt caggaactga agtgaaatcg ctctttgcgt tcatcttctg
       61 tctgggtgct tttccgctcc atgcaattgt gcccgtacct cctttgcagg gcagagttca
      121 ggatcttaca gaaactttaa ctgcagcaga gaagaccgcg ctggagcaga agctttctga
      181 ctttgaaaag acaaaaggct cgcaggttgt agttctgatc gtaccttcaa ccgaagagga
      241 ggccatcgaa caattttcga ttcgcgtcgc ggaagcctgg aagatcggaa gaaaaggcgt
      301 agacgatggc gtgattcttc tcgttgcaaa gtcggacaaa aagttgcgaa ttgaagttgg
      361 ctacggactg gaaggggcac tgcccgacgc aatctgcaag cgaatcattg atgaacagat
      421 cgtgccgcga tttcgcgcag gcaaatttgg agagggcatc actgccggag tggattccat
      481 cttacaacgc atcaacggcg aacctctgcc ggctcctttc gtttcaaatt cgcaggacct
      541 gactggaatc ccgtggacca tgcaactcat tgtaactgca ttcttaagta tttttatcat
      601 tgttggatct cttgttccct cagccgtgaa taagtttctg tgcattctcc ctgttgcagc
      661 agggctcgcc gccgcagtgg ctggctatgt agtgggaggt cattggatcg tggctgtaat
      721 gttcgctgct tttgccacgt tctttgttgg tgtttttact acggtgatga gtggattctt
      781 tctctccatc tttggattga tcacaggcaa gaaagtaggc ggatggagga tcagcacctc
      841 aagtggatcg agcggatgga gctcgtcgag tggatcgagt tttagcggag gcggcggcag
      901 ctttggtggc ggaggagctt ctggaagctg gtaggcggaa cgattgatgg gtcctgtacc
      961 ttctgatgtt gtcaggagga accatggcaa catcgacaga aaagaaaaaa caaacggatc
     1021 gggatctgtg gcgagagctc acaacaagaa aagaagaatt tagcaagatc gtgaagttac
     1081 tcgttgagtt cgacgaaaag accggtcgct atttgaagta caatcatccg gaaactcact
     1141 atatgcggcg cgctgtggca gcatgcgatc cggagaacct gcgcattttt ctaaaatctc
     1201 tgggtggctt cgtatattcc gttgtggctg aggtgaaaag tgccgctggt ttgactgcga
     1261 ctgcctgggt tcatgaagat ggtattcgcg aggaaaggga aggttgtgac acaggccatc
     1321 cagtgcacca gatcgtttgc gtcacagacc tatttgaagc ggattctcag acgatcgacg
     1381 tggttcatag ctgcgtttcg caacctgccg cggacctgct ggcagagaga ggcgaataaa
     1441 aagtacgtgc ttccaaacaa tcgcgcccac accctgggcg cagatgattg agatcactgg
     1501 actcaagaaa tcattcaaag gaatgccggt tctcactggc ctggatctga ccatccagga
     1561 cgatgaaacc atggctgtca ttggaccgag cggatgcggc aagagtgttt tattgaagca
     1621 tctcgttggc ctgttgcgcc cggacgaagg taaaatcatc atagatggag aagacatcac
     1681 tcgctattca gatagcaagc tttacgattt gcgcgagaag ttcggcatgc tttttcaaag
     1741 cgcggcgtta tttgattctc tttccgtagg cgaaaacgtc gggctctggc tcaaagaaca
     1801 cacgaagacc cccgaaacgg aaattgctgt gaaagcggat cgcattctca acctggttgg
     1861 cctgcgcgat gtgatggaaa aacgtccttc cgaactgagc ggtgggatga agaagagggt
     1921 aggtctggca cgcgcgatca tcaacgaacc taaatacgtt ttatacgacg aacccacaac
     1981 cggactcgat ccaatcatgt ccgatatcat caacgacctg atcatcaaag tacgcatgga
     2041 attgaagaat actgccattg tagtcactca cgatatggcc agcgtgtaca aagtcgcgga
     2101 tcgcattgcg atgatgcatc agggtaaaat tgtttttctt ggaaagccag acgatatcaa
     2161 gacaacagac aatccgctgg cgcaacaatt catacagggc aaagcacagg gaccgattcg
     2221 gctatgagtt gcctggccaa acaaattcgc ctatattgaa caaaggaaag tccatgcacc
     2281 agacgatgat cgaactcaag gcagtgaaga agcgtttcgg agctcgcgaa attttgcgcg
     2341 gagtggacat agacattcgt cgtggtgaga cgatggtttt gctcggcggt tctggaacag
     2401 ggaagtctgt aacgattcgc catatcattg gtctactgga gccggatgaa ggtgaagtgc
     2461 gcgtagatgg ccatttgatg agcggggcaa cgaaaaatac aaagacaaag cttcgcgcca
     2521 gaatgggagt gctctttcag agtggagcct tgatcaactg gcttactgtg tacgaaaacg
     2581 tggctcttcc tctgcgcgaa acgtctaaaa agactccaaa agaaattgat gaaatagtgc
     2641 gcgaaaagat cgcatggctg ggtttgacgc ctgctatgaa tttgcttccg gacaagattt
     2701 caggaggaat gaagaagcgc gttggagtag cgcgcgcttt gactactcaa cctgaaatca
     2761 ttctgtacga tgaaccaact tctggcctgg atccggtcat gagtcaagtg ctcaatgaat
     2821 tgaacaatcg tttgcgtgat gaactgaaag ttacgcaggt ggttgtgacc catgatatga
     2881 attccgcttt cacaattgct gatcgcataa cgttcctcta ccagggaaaa gtgcgatgcg
     2941 tcgggacccc tcgggatata caagaatcaa cggatcctgt cgtgcaacag ttcatcaagg
     3001 gagaaactgt gggtcccatg cagatcgacg cgggtgatcc cacagcaaaa aaggcggata
     3061 gttagcgcgg tccggactgc agagctcttc ggctattgat aaacgttccg tactttctgt
     3121 cctccaccag aatatgattg gtaagccaga gccatatggt tccgcacatt tcaaacgcga
     3181 gaaaacgtaa atcctcgcca tcatggatgc gagccccata tccttccact gtctttacga
     3241 agtctgcatg aagctgcatg tgttcgtcca gtgccggata acggtgatca tgcatgaact
     3301 gttcttcgct ggaaaggtgg aatttacagt attcggaaag atcatccatc aagtgtctca
     3361 tctgctctgc atctgagccg ccttccttca ccatccaagt caactgattc atgatttcaa
     3421 aaattttcgc atgttccgca tccatttggg ggaccccgac tgaataggat tcagaccact
     3481 ggagcactct tcccacttca ggcattttga aacgagagag cactctttct gccatcaaca
     3541 ttagatttcg gatttgtcct acaatgcggt tcaaacgatc cgcatcttct gccagaatcc
     3601 gggaagagtt ggatgatgtt tccacgcctg ccgaaatctc ttgcgtggtt tttctttgct
     3661 ctcgcattgc ctggtcaatg tccccggaag aatccacaat ttcagaagct tctttcgtga
     3721 tctccaccgc gctcctgtct tgagatttga gtgactctcc aatttgttca aatgcggctg
     3781 ccatctgccg ggttccatct tcgagtcctg taagaattcc cagcacgccg cgcacacttt
     3841 cgctgctgga tttcatttca gtagaagtct cctcaatgag tctcgcgatt tcttccgcac
     3901 tcgtggccgc gtggtcggca agtttggaaa tttcggaggc aactactgcg aacccacgac
     3961 cgagatcccc cgcccgcgcc gcctcgatgg atgcattcaa tgcaagcagg ctcgtgcgtt
     4021 ctgaaattcc gttgatgact ccaacaactt cgtgaattcg ctctgctctt tcctgtacat
     4081 gttccatggt aacgaggcct tcgtccatgg atcttctgcc ggcggctgct cgcgtaagcg
     4141 cttcattgga aatagaacga gcttgatcca tattccggga gatttcgtgg atgctctttc
     4201 ccaggtttct tacgttttct tcaattcttt tgctaacgag tgcgaggcgc tccgcgcgct
     4261 cgcctacatt ttctgccgct gccgccagct cttcgagtgc cgccgatcct tcttccgtgc
     4321 ttgccgcaag gtgttccgct tctgtgctga ggcgagaaga agatcgttcg aggtcatttg
     4381 tctggctttg taccagaacg gattgttctt ttacctgaag caccagaaac gaaacgcttt
     4441 cgataaaggc gttcacccac gacgcgattt gtcgcatttc ctcggcgttt cctgcaggga
     4501 tgcgagcacc cagatcattt tcctggaggg ctgtgcgaag agaatctcgc agtgcataca
     4561 cgggcacaat gaatccgcga tacattccga tggcggctgc cagaatcagg atcaacgtga
     4621 tgatcagctg gatgatgtta gaataccagg caagcgatac ggatttcttg atgtccgctg
     4681 acgacgcatc gacgtgcgac aggctttgtg tgcgaagctt gcctactcgt tctgcaatgg
     4741 aatctgcctg cgcgtcaaat cgtctcatcg agcgattgcc acctgcttga ccttgtgtca
     4801 gatacgcttc tgtcatctcg gaaccaagat catagtaacg atcaaattct ttttgcagag
     4861 cgtcgaaatc catgagcttc tcgttgccgc gaagctttcg aaactcggca agagctgttt
     4921 tgaattccgt gcgcgcagtt tcagcgtctg aatagccgga tcgatcgcca gtgaccgaca
     4981 catccgtaag ccattgttga acttcaactg ttgcgacaac catacgatcc gccagaacga
     5041 cagacgggat ggcgtcttcc tgtacttcct gtagttcagc gcggatagaa cggatttcat
     5101 acaaatggta tagcgcggtg agggaagtga tgacggccag gatggttaac ccgccataga
     5161 aacggacgcg aaggctcata gcgcacagcg gttcgtattg cctgattttg tgcaaggatt
     5221 tcgcatgcga aacttgcttg cccgatcagt tgccagcatc tacaagagca ggtgcggatc
     5281 cgacgtttcg ttttgccggt tttcgttgcc ttgatttgtg cgatcctggt ctatgtctac
     5341 atgaagacgg ggcatggcac gtcgcgggcg tttcgcaata tttgctttgt tgagttcttt
     5401 gccgcgaccc tcattgcgac ggtttcgccg cgatggagat ttgttttcgc cggcttcgcg
     5461 accttgactc tgtccaggct gccgtctctg ctactgcaga ttccaacgca caatttgctt
     5521 tttccgattt tctttggtct ggctctgggt gcctactttc gcgcagcgcg aaatgatctc
     5581 tcggagccag tagctcggtt cggagtggca caaatccttg cagtcgcgat catcatcgga
     5641 tgtgttcgcg ccattttgca gaactactgg gagccgggga ttggacttgt cctggaaaat
     5701 agaatcctgt cggaccaaat gacatccaac tatgccgtct ttctggcttt gactctggga
     5761 ttgcatttgc tcgcacctat cgtctggatt tgtgtcgagc aagtgaatcc agagttagaa
     5821 gcgggcgaca tggcttcccg gctctcgatt ggaatcctct ggggaattgg ggcgaatctc
     5881 atcgtcatgt tactccaggg atttgtttcg ccactgaaca ctggaatcac ggatcgcgca
     5941 atcgaagcgg gaaggagggc aggattgttt tctgattcag gatctgccgg tgccgcgttt
     6001 cttgctttcg ttttgcctgc gagtttcgct ttcgcttcgt tgtttcacgc caggatcaag
     6061 aaaaataaga gatggttggc ttctttgctc ttcttctggg cgattgcgat tttcacagcg
     6121 tcatttttcg ggcgcgtgat tgtgctagcg agcgtgggcg ccctggttgc gtggctcttc
     6181 ttgattcctt tgaacaggcg cgttcgcctc attgcaggct tcgctatttt gttttctttt
     6241 gtctgttttg gacttttcct gatctggagt gggcctgttg catttttgtc gcgcatagat
     6301 cccgttcgcg ctcagctttt ggaaacagga atcgttttga ttcggcaaag tccctggctt
     6361 ggacatgggt tcaatagctt cgttcccgct ttaatggagc tgcgaaaaag cgatcccacc
     6421 attctcatag aaaacccggc aggattgatc ccaggaattc tcaatgatgc cggaattgta
     6481 ggtctttgca ttctggttgc aatgcttgta tactatgtgc taggattgcg cttgctgttc
     6541 ttgagaaaag gagcagatga aaatgctgct gaagccaccg ttgtggtgat cctttccact
     6601 ctctctatgt ttcttgtgtt tactgtgggg taccacattc tcgcggcgga ggtggcagcg
     6661 tttgtgtttt tcccgcttct ggttctgccc gattcattca ggcgaaccaa aagctgagcg
     6721 gcgcatcaaa gacgacggcc gctgcatcat tacttaggtt ttttgtcttg ttttggtttt
     6781 ttcttttcgc ggttgccttt gtttttttcg cccatttgaa tgctccttga attcaatcga
     6841 actgctaaca tgcttgagtt actttgagcc gcggtgaagg caattaaaaa tcattttgtt
     6901 tctcgcgttt gccattcgat atcacttttg ccagcgcgaa aatttacgta ccttgctgcg
     6961 atgaaaagat agtcggacag tcgattgatg aatttgtacg cctccggata tacgacaggt
     7021 ctttcacctg aattttcatc aagtgcagct gtcatggctc gctccagtct cctgcacaca
     7081 gttctgcaga tatgcaatgc ggacgcggcc gcttctcccc ccggcagtat gaatgcacgc
     7141 aacggaggta gcactgcagt gcactgatcc atccattgtt ccagcttgcc tgtatcctct
     7201 tcgcgtatga tggaaccttc tttgttcttg taataaccgg cgagctctga cccaagttcg
     7261 aaaagaagat gctgcagatc cagcagctga gcgcgcgtct cttccgggac gcctggctga
     7321 gaaagggcca ggcctatgtg agagttcagt tcatctgccg tgccgtatag atcgacacga
     7381 acatcgcttt ttctggtttt ttctccattt gcaagtctgg tccaaccagc atcgccctgt
     7441 ttcgtgtaga ttttcatagc accccacaat cgagcgagtg cacctgcgtt caatattttt
     7501 tgcacattgg ttgcttcgtg tcgatcgcaa cattctatgg atcatgccag tttcttcgga
     7561 attgatggcc aggagagaga gactgatcca tcttttgtct ccgcacactg tgcccggtct
     7621 gattgtggct ttttcgggtg gagtggacag tgcgtgcctg ctctgggcgg ctcgtgaagc
     7681 tgccaaaaag tccgccggcc gcgttcttgc cattactgcg gtcagcgcaa gcgttcccga
     7741 tctggatttg cgagatgcgt ctgagtttgc agagaagctc ggcgtggagc acagaattct
     7801 gcggagccgc gagacggaga accaggaata tttaaagaat gattcgaatc gttgctttca
     7861 ttgcaagacg gaactgtttc ggattgcacg agaagaagca gatgcggaag gaatccagca
     7921 catagcgtat ggttatacga tctctgatcg tggcgacgtt cgtccaggcc atcgagcggc
     7981 cagaatggcg ggtgttcttg ctcctcttga ggaagcggaa ctgaccaagg aagatatccg
     8041 ggccattctg cgcgaagaag ggttttcact ttctgaaaaa ccggccgcgc cctgcctttc
     8101 ctctcgcatt ccgaccggtc ttcccgtgac aatcaagaga ctggaagacg tgcagcaagt
     8161 ggaaacattt ttgcgagatc acggagtgca gattgtccga gcgcgaattc acgatgaaaa
     8221 gacgattcgc atcgagacag aggatgcgtc cgcggcaaaa gtactcgaat tgcgacatga
     8281 tcttgagaga ctatcccgat ctctcggcta tcggtggata acgctggacc tgggcggcta
     8341 caggacgggc ggttcgactt gaatttgaat gacgttcgca agctgctcga gcgtttgatt
     8401 tccggcgaag agaatgtgga cggagtcatt cgtcaattgc gcgcaggacc acttcgcatg
     8461 gagcggactg cagatggatt tccagatcat catagacttc ttcgcacagg catggcagaa
     8521 gcgattttcg gagaatccaa atcgattgag cagataacgt ctcttgccag atcgctcagc
     8581 gaaaacggcg cgccagtact gatcacaaga ctggacgcgg ccaaaaggag cgctctcctc
     8641 gctttgtttt cgggatcgcg agtgagcgat caggcaaaaa caatcttgat tcatccaccc
     8701 gaaattcttg aagtccaggc agtggatgct ccatcctacg cagagccgtt tgccgccatc
     8761 gtgactgccg gaacatctga cattgcagtg gcagaagagg cggtggaagt gtgcattgct
     8821 tgtagcattc cgtttctacg gattcgagac gccggggttt ctggactgca tcgcattctt
     8881 tcttacgcgg aagcattgca gaatgcatct gcgatcgtgg tttgcgctgg catggaagga
     8941 gccctgccag gtgtagttgc agggatgacg ggtagaccga ttctcgcggt tcctacttcg
     9001 gttggatatg gcgcgaacct gggcgggttt tccgctcttc ttacaatggt aaattcgtgc
     9061 gctgccggag tgtccgtcgt aaatatagac aatggttttt ccgcagggta tgctgcagcg
     9121 agcgtcattc gagaaattca tagattccag agaaagaact aacgttaagc ttttggcggt
     9181 tctgccgatg cgtggagtat gcaggttgca gaacgatcgc aaacatttcg acctttcgaa
     9241 gcgactgttg cgtgtcccga ttgcgggcat acgattgatc tgtgggtgca gccaatgcac
     9301 agggaaaatc gcactctctg tccagtctgc agcagggccc tgactggaat cattttgcgt
     9361 ttgattgaaa aggaaactat ttcacaacgc gcgctgcgcg gctaagaagt tcttcgagat
     9421 gcttcttgtt cctcgcttcc gcgatgatac ggatcacagg ttccgtgttg ctggaccttg
     9481 catgaatcca tccttccgcg tctgagagat ggaggccgtc tttgcggtct gcccgcattc
     9541 ctggaaacag tccttcgatt ctggaaaaca actcccggac atccgttcct gcttttacgt
     9601 cgaactttct cttctccatg aaaagttcgg gtagatggtt cagaacatcg tctaacgttg
     9661 cggcattttt gcgtgccatc gcactgagaa tcagagccgc acccagaagc gaatcgcgtc
     9721 caaaggatgg cacgcgagag tgaatgatac cgccgttccc ttctccgcca aagaacgatt
     9781 tattcttctt catcatttct acaacgtttg cttctcctac ggcactgcga atcacaggat
     9841 atccgtaagg tgcggctact tgatcaagca gagtcgacgt ggagagattg actacgaatg
     9901 gagtcttgcc gcgtggctgt gatttaggaa cgtctttgat ttccgggaga cccagaaatg
     9961 acagaggcaa agagtattct tcattgatgg cgccgcgtcc aggagaaccc ggaacgattc
    10021 gatctgcatc cggatcgagt gcgaagccaa cagcggctga ctctttttta agcaactgac
    10081 tgaatttctt taaagcaacc ggcgtgggtt ccggaggtcg cggaaaatca aaaccggagg
    10141 ctgcttcgca aaaaagagga atcacttcgc aaccgagctc ggtgagaagt tcgggcaatg
    10201 cgtttcttcc cgctccaccg actgcatcaa caacgacttt gtatttcttc gctctgattt
    10261 gttctacatt gtttagaaga tttaagaccg tttttacgtg ttttgaaact gcgtcgattt
    10321 catggaagga tcccagcgtt ttgtgatcgg cacctgtcag tttgccgctc tcgagcgcgg
    10381 tcatcataga agaaagatta aatttgttga agaagaaacc ttccgggccc atgagtttca
    10441 atccattcca ttccggagga ttgtgactcg cggagatcat gataccgcct gctgcatgtt
    10501 cgagtttgac agcagccttc actgtgggag tgggagcgag gcctgcatcc agaacttcct
    10561 ttccttgcgc tcgcagagta gcgcatacaa gatcgcgaat gagcggtcct gaatttctcg
    10621 agtccatccc aatcacgatt ttttttcctg tgactgttcc gaaaccaagc actgcatcta
    10681 cgatgacaga aggactcagg ccagtcggga gtattccgcg aattcctgaa acagagatca
    10741 tgagttgggg acttgctacg agacgatgtt tggccatggg ggatgttgaa aaatggctgg
    10801 accgggcgcc tggtcaaaac ttttctagtc tctccaagtt ttctgcgccc ggatggtgga
    10861 attggtagac acgcaagctt gaggtgcttg tgccgtaatg gtgtgggggt tcaagtcccc
    10921 cttcgggcat ttcgtaaacc acgtttcacc cacgagcaca tttacacgca cactcacgtt
    10981 cacgtcacgg gactagaacc acctgggttc gctgcgcgaa catcaggcac ctcactccct
    11041 atgggtcgtt cggtgggttc gcgccccctt cgggcatatt ccgcgcacgt tttcggacac
    11101 cgcacacatc actgctcgtc acgttcacgg cacgggacta gaaccacctg ggttcgctgc
    11161 gcgaacatca ggcacgtcgc tccctatgaa ctatgaaaag acgaaacttc ggcatacaat
    11221 ctgagagtct ctaagacacg cgcagttagc tgcagcgtcc gggtgcagtc cacagcggca
    11281 ttcggagcgt ccgagcgtcg caacggcgcg tcgcgaggat ggactgcatc cgggcgtt
//
LOCUS       DAOQJL010000053        11304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2908_length_11304_cov_4.249444, whole genome shotgun sequence.
ACCESSION   DAOQJL010000053 DAOQJL010000000
VERSION     DAOQJL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_11304_cov_4.249444"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1658)
                     /locus_tag="PLG78_03800"
     CDS             complement(102..1658)
                     /locus_tag="PLG78_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22261.1"
                     /translation="MEFLRRHCPYVLFSIPLIVSEVTALICNTAFGALIGSYLLGQPG
                     IYDASAASIVLGLLEVSSFVLSFVFLYPVFIASSRRKRGEEVPASLLKQARTRIYNFP
                     PFLIMLIWGVSFLEFLVTILSPELRKFGHFIPFVSYVLVAVSINAIVIYYLADSLNRF
                     VFFPDWFPDGNFDVTFRLRSPSLGFRFFDLFLVTGIFPVVTILGTFAVSRMQLAWDPD
                     AGRRALIVGVVMGGLYWLLGFVLLVLNSQSFLKPLNSMARAVKDLENAKYETRVQVHS
                     DDQIGNLERAINDMSRAMREKEMIKRVFGHYVSPAVRDMILDGKINRDGDRIEAVVLF
                     SDIRSFTALSEKYEPEKIVKLLNIHFSRVVDVVSKNNGFVDKFIGDAVMAVFDAELTD
                     NMHRLCALKTAAEMLQGVEQTNADLAALGIDPIRIGVGLACGSVIRGNIGSADRREMT
                     VIGDIVNLASRLEAATQELGCPIVATKDTFDESCKEIQLLRILETRSFIVRGRSQPVD
                     VLTMTMHDPA"
     gene            1730..2524
                     /locus_tag="PLG78_03805"
     CDS             1730..2524
                     /locus_tag="PLG78_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNE22262.1"
                     /translation="MKSRIILRSQREVLMSPIILLPVIVLGLMFLTQAIKILREYERG
                     VVFRLGRFSAVKGPGIIILIPIIDKMVRVSIRTIAFDVPPQDVITRDNVSIKVSAVVY
                     FRVMDPDRAIIQVEDYLYATSQLAQTTLRSILGESELDEILSRREKINAELQLVLDKQ
                     TDAWGIKVGIVEVKQIDLPQEMQRAMAKQAEAERLRRAKIISAEGEFQASAKLSEAAA
                     VIEKHPTAIQLRFLQSLVEIAADKNSTIVFPVPIDLIHSILEKMKK"
     gene            complement(2525..2836)
                     /locus_tag="PLG78_03810"
     CDS             complement(2525..2836)
                     /locus_tag="PLG78_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNE22263.1"
                     /translation="MHFEKHVFVCENKRDPSAPRPSCGHKNSTQLRNFMKDKVKEMCP
                     GRSIRVNMAGCLDRCEEGPSVVVYPDGKWFKIETEEQVVEFIRETLIEGKTSTHLSMK
                     E"
     gene            complement(2836..3318)
                     /locus_tag="PLG78_03815"
     CDS             complement(2836..3318)
                     /locus_tag="PLG78_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE22264.1"
                     /translation="MKILLIRHAIAVAREEFQGSDLDRPLTDKGKKRARRAFAGLERI
                     YPGIQKIFTSPAVRAHHSARLLKRQYPEAEVHLSDLLLPEAQDDDSILSLIQSHMAET
                     IAIVGHEPNLTRLAGTMLGSHSLPFELSKAGVLVIETDETGSRMESLLTPRILRRVYG
                     "
     gene            3455..3985
                     /locus_tag="PLG78_03820"
     CDS             3455..3985
                     /locus_tag="PLG78_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE22265.1"
                     /translation="MQFCSSCGKTLSLRIPEGDNTMRHVCDSCGAIHYQNPKIVVGCL
                     PVYEGKILLCKRAIDPAYGKWTLPAGFMENGESLEAGAARETREEAEAEVRILRLHSV
                     FSIPHIHQVHFFFLAEILDGRFGAGKETLEAKLVTAEEIPWDEIAFGSVRFALKRYLD
                     APHQMETHVDSYLKPQ"
     gene            4050..4292
                     /locus_tag="PLG78_03825"
     CDS             4050..4292
                     /locus_tag="PLG78_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22266.1"
                     /translation="MKKLTAATMLAVTLAVGFALGTLSAENQPHMKAALSQLGQAKDS
                     LQKATADKGGHRAKAISLVNEAIEEVKKGIAADNAR"
     gene            complement(4337..4543)
                     /locus_tag="PLG78_03830"
     CDS             complement(4337..4543)
                     /locus_tag="PLG78_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22267.1"
                     /translation="MAYLKFSDATGKIMADEQKDEKKNKKVNRMTLSEVDAAIKKTQE
                     HMKGMNSKYAKELVGRQAELKAKK"
     gene            4542..5531
                     /locus_tag="PLG78_03835"
     CDS             4542..5531
                     /locus_tag="PLG78_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22268.1"
                     /translation="MKTSRIPAVLLLALSACAHSPGGYYFWKSEWKLEEKNLPYLNQS
                     RFAYLRMFDVALEGGEAKPSGTIRFASRWPEHLEAVPVVFITQEAIKAQNDSQVPVLA
                     ERIHRLMRNLEQSGSLPEIREWQIDCDWSAKTESRYFTLLKQLKRLEPQRRLSVTIRL
                     HQLSTSVPPADRGMLMLYGFENPADYRQANSIFDEKRAAPYLRKLKSYPLTLDLALPV
                     FSWAVVFYQGSFRHLIRDNTASLRQADGLTDLSAPWSRSQWFRAEKNLWIGPDRIPAG
                     AEIRLEETGPAIARRAKSLVDLSAPPFASDSRIVLFDFHSAQNYSVSEIEAIF"
     gene            5528..7792
                     /locus_tag="PLG78_03840"
     CDS             5528..7792
                     /locus_tag="PLG78_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22269.1"
                     /translation="MKSKLILSVLAFALSFPSAILPCGPSSGLADPFLFSEIPSSAYG
                     RVCIDSLLIDKSSMPVACGAITREEENAREWAAYLGITAAEARKGVYENPKLPAFTRA
                     LAKKPGASEYLALARSVEKVQLSTVGWDYDFDREKEKSDLSQTKSILKDLQKKAATTN
                     DPFLSPRYNLQKAKLLLRLGNYSEILSIPVPENPGFTRSQILRLRSGASLRMKEKVTA
                     AIQTILAYEASPYSGAADDFRYLLLEEKDYRQIQSSNLSKKQVGIFYFLSGIHMSSGG
                     SFSLAVEDLENLWNAEPGTPYADPLLLKTIALMEEESLPVILYHQEPPKQNSGIDSIR
                     IRLLSWIAGKFPQLYAQTNANGLPDSTTLAATRLRKFLEKNKSNSRNPALWNLAAAYA
                     AYTAGDSDAASAALSAKEKKPAYEVRRQLILLLARIQKERVISEETENAFVNLLPLLS
                     GSSSGMARYSPFDEKGPPAATMMLYELLSDKYKKGGAGHKGIIASYLAGAFEWYDTAR
                     LDASSEPDLVRLRDYLAQPPVTSMDRLIKSKFHLSRAVVMDRLAAKYALQGKYKEAEQ
                     ELRAAGPPKKITLEYTGDLGPFDATFEDASKNAKNWNRLTFFQEMGKLSAAIDAKPAD
                     AEAALLHYRRGVGALSASHFGKWWILMKDYRSVGDVYMGHDWELMLRQAKNDFEAAAR
                     LTKDPELGAKSTFLSALASQGLNQPREWTEEKQKLSAAEADSYRKLGTIYKDTRFFNT
                     IGSSCSVLNDFLGH"
     gene            complement(7818..8711)
                     /locus_tag="PLG78_03845"
     CDS             complement(7818..8711)
                     /locus_tag="PLG78_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE22270.1"
                     /translation="MLVRAEGRMRTAMKILAVSTVVNVALNPLFIRTFGLGIRGSALA
                     TNVAMVALAAAGAVYFRSGKSSVREPVSLFAYDPKIFKRIISIGLSSFILQGMGVLQQ
                     IFVFRLIAAHGTEKDVALMGTGLRILFLCVYPVMGLVRALQPVIGINFGARQYARVIE
                     ALRVFSIGGTAFMALIWLPLTMFSRTVLGWMLPDTAITDTDVLNFRILIVTLPLMAGV
                     FNGLTFFQAIGHGKTATILAVSRQILVFVPVVLILPVYFGVSGIYYSLTGVDVTLIAV
                     LAMLVLREFKLLSAKPDGAER"
     gene            complement(8695..8997)
                     /locus_tag="PLG78_03850"
     CDS             complement(8695..8997)
                     /locus_tag="PLG78_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22271.1"
                     /translation="MSSGFKDTEDYILRGGLWRLMVDLAWPAIVGMSFYALNTFVDVI
                     FIGQLVGEQAVAGVSLAFPLASIPMGVAAMCPVFFGSFLVLSSHSLRNLSEHAGTR"
     gene            complement(8999..9757)
                     /locus_tag="PLG78_03855"
     CDS             complement(8999..9757)
                     /locus_tag="PLG78_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22272.1"
                     /translation="MRRAFISAFVAALLGVAFPRFAETTQTAPQPAKESEDKARRCLN
                     PATRTAQVPSDGRPIMSENAIGNNPQEKQYREEIERLLTIEIQNVKIAVTDVELKHKR
                     LMQLYPRLQYYQEIVLEDVPGAWREGDYVNSKKVLSLTYDLPADGSSKLTCLVMDSMT
                     RNVYHPDQWTRKLMRLYYPNVQMVELETHRHNFSMPSPGAPAITLEATSPEIQLRALR
                     LIYLNLRTALYSMDMLIASYYDKRNRRNEWQINL"
     gene            complement(9862..10269)
                     /locus_tag="PLG78_03860"
     CDS             complement(9862..10269)
                     /locus_tag="PLG78_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22273.1"
                     /translation="MAGLACLPSLEEMRLLAPFFAFVLAASCAWMGEDGLTAIRGQKV
                     ITRKETQGRLREAIIINMAKCPENVNAGLYGEDYIVPGEVTHTHYYEESVNNCVVAIL
                     AVSCGLNPSQNIVILANFYRTVIRLCNLKPATG"
     gene            10327..>11304
                     /locus_tag="PLG78_03865"
     CDS             10327..>11304
                     /locus_tag="PLG78_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNE22274.1"
                     /translation="MSPKQKKPTASAPGAKKKVPADLPKEAWSAARIRKLWMDFFKSK
                     KHMVLPSASLLPSGDPTLLFNTAGMVPFKAYFAGTEKPPSPRVATIQKCLRTTDLESV
                     GKTERHCTFFEMLGNFSFGDYFKRESIQWAYEFSLKHLNLDPEKIYITVYQDDDEAEE
                     IWHKEVGVPMNRIRRLGKDANWWGPAGDSGACGPCSELYLDRGEERCNRKPDASCGPG
                     CSCDRFMEYWNLVFNQFHQDTSGKLHPLPMTGIDTGAGLERITMLKLEENSVYDTDEM
                     KQIIRFIEGLTETLREDKKKVEYDPTNKHTAPSFRVLTDHTRATTFALSD"
BASE COUNT         3109 a         2944 c         2687 g         2564 t
ORIGIN      
        1 gtgcaacatc caggacggaa ccatttccat tatttcagga gatgatctga tcgacgtaga
       61 acctgcagta cttcacggaa cctggccatc cagaaactga gtcatgcagg atcatgcatg
      121 gtcatggtga gtacatctac aggttgcgat cttccgcgaa caataaacga tcttgtttcc
      181 agaatgcgaa gcagctgaat ttctttgcat gactcgtcga acgtgtcctt tgtggcaaca
      241 atcggacatc ccaactcctg agtggccgct tccaggcgac tggccaggtt gacaatgtcc
      301 ccgatcacgg tcatttcgcg tctgtctgcg gaaccaatgt tgcctcgaat cacgctgcca
      361 caggcaagac caacaccaat tcgaatcgga tcaatgccca gagccgccag atctgcattt
      421 gtttgttcaa ctccctggag catttctgca gccgtcttaa gggcgcacag tctgtgcatg
      481 ttgtccgtga gctctgcgtc aaatactgcc atgacggcgt ccccgatgaa cttatccaca
      541 aatccgttgt ttttgcttac aacatctact actctagaaa aatgaatatt taaaagtttt
      601 actattttct ccggttcgta tttttcagat aaagccgtaa aagaacgaat gtcagaaaat
      661 aagacaacgg cttcaatccg gtccccatcg cgattgatct tgccgtcgag gatcatatct
      721 cgaacggcgg gagacacgta atgcccgaag acgcgcttga tcatttcctt ttcgcgcatg
      781 gcacggctca tgtcgttgat ggctcgttcc agatttccaa tctgatcatc gctgtgcacc
      841 tgaacgcgag tctcatactt tgcgttttcc aggtctttta ccgcccgggc catgctgttg
      901 agaggcttta gaaaactttg cgagtttaga accagaagca caaatccaag aagccagtac
      961 aggccaccca tcacaacgcc cacaatcaag gcccgtcttc ccgcgtcagg atcccaggca
     1021 agctgcattc tagaaacggc aaacgttccc agaatcgtaa cgactggaaa aattccagtg
     1081 accagaaaca gatcaaagaa tcgaaatccc agagaaggag aacgaagccg gaaagttacg
     1141 tcgaaatttc catctggaaa ccaatcggga aagaaaacaa agcgattgag actgtctgcc
     1201 aggtagtaaa tcacgattgc attgatgctc acagcaacca gcacgtagga aacaaatgga
     1261 atgaaatggc caaacttccg caattcagga gaaagaattg taaccagaaa ctcgagaaag
     1321 gaaacgcccc agatcagcat aatcaggaaa ggaggaaaat tgtagattct agtgcgcgcc
     1381 tgtttcaaga gactcgctgg aacctcttct ccgcgcttac gcctcgaaga tgcaataaac
     1441 acgggatata aaaagacaaa actcagaaca aaggaagaga cctcaagaag tccgagtaca
     1501 atggaagcgg cgctcgcatc ataaattccg ggctgaccga gcaaataact gccaatgagt
     1561 gcaccgaacg cagtattgca gatcaaagca gtaacctcgg aaacaatcaa aggaatgcta
     1621 aacagtacat acggacaatg ccggcgaaga aattccatct gcttgcaaga caagcacgtg
     1681 gagagcgcat gctgtcaacc acttgcccgc agatgaatct cttgacgcca tgaaatcaag
     1741 aatcatcctg aggtcacaaa gagaggttct catgtcaccg atcatcctac taccggtcat
     1801 agtacttggg cttatgtttc tgacgcaggc catcaaaata cttcgcgaat acgaacgagg
     1861 tgttgtattc cgtctcggaa gattttctgc agtcaaaggc ccgggcatca tcatcctgat
     1921 tccaatcata gacaaaatgg tgagagtgag cattcgcacc atcgcttttg atgttcctcc
     1981 acaagatgta atcacgcgag acaacgtttc tatcaaagtg agtgctgtag tttacttccg
     2041 cgtcatggac ccggaccggg ccatcattca agtggaagac tatctctatg ctacgtctca
     2101 gctcgcgcaa acaacactac gaagcattct gggagaatca gaactggatg aaattctatc
     2161 cagaagagaa aagatcaacg cagaacttca gcttgtcctg gacaaacaaa cggacgcatg
     2221 gggaatcaaa gttggaattg tagaagtcaa gcagatcgac ctgcctcagg aaatgcagcg
     2281 cgccatggcc aagcaggcag aggcagagag acttcgccgc gcgaagatca tttccgcaga
     2341 aggagaattc caggcatccg ccaaactctc agaagccgca gcagtgatcg aaaagcatcc
     2401 aaccgccatc cagcttcgct ttctgcagag tctcgttgag atcgcggcgg acaagaattc
     2461 aacgattgtg ttcccggtcc ccatcgattt gattcactcg atacttgaaa aaatgaaaaa
     2521 gtaattactc tttcatggaa aggtgtgtgg aagtttttcc ctctatcaga gtttcgcgga
     2581 tgaactccac cacctgctct tccgtttcta tcttgaacca ttttccgtcc ggataaacaa
     2641 caacggacgg accttcctcg caacggtcca ggcatcctgc catattgact cgaatgctgc
     2701 gacctgggca catttccttc accttgtctt tcatgaagtt gcgcagctgt gtgctgttct
     2761 tatgaccgca ggatggtctg ggcgcggacg ggtccctttt attctcacaa acgaatacgt
     2821 gcttttcgaa atgcatcaac cgtatacacg gcgaagaatg cgcggggtca acaacgattc
     2881 catcctggat ccggtttcat ctgtttcaat gaccagaacg cctgccttgc tgagttcaaa
     2941 cggaagagag tggctgccga gcattgtgcc tgcaaggcga gtgagattgg gctcatgacc
     3001 cacaattgca attgtttctg ccatgtgaga ttggatcaga gatagaatgg aatcgtcgtc
     3061 ctgcgcttca ggaagaagca aatctgagag gtgaacctct gcttccgggt actgtctctt
     3121 gagtagtctt gcagaatgat gtgctctgac tgcaggagaa gtgaaaatct tttgaatccc
     3181 gggatagatc cgctccaggc ctgcaaacgc ccttctggcg cgcttcttcc ctttgtccgt
     3241 cagagggcga tccagatcag acccttgaaa ttcctcgcga gccacggcga tggcatgtcg
     3301 tatgagcaga atcttcatag atccatttct cagatttgag actttcgccg ggactgtttt
     3361 tctgcgaatt gtattacaat tctgagtttt acgtagacgg aaaagaaatt gaaggcatcg
     3421 attctgcacc gattctggcc ccatgctcgc cgccatgcaa ttctgctctt cctgcggcaa
     3481 gactctgtcc cttcgcatcc cggaagggga caatacgatg cggcacgtat gtgactcctg
     3541 cggcgccatt cactatcaga atccaaaaat cgttgtgggc tgcctcccgg tatacgaagg
     3601 taaaatccta ctctgcaaac gcgccatcga tcctgcgtat ggaaaatgga ctctacctgc
     3661 gggcttcatg gaaaacggag aatccctcga agcgggtgct gccagagaaa cccgtgagga
     3721 ggcagaagca gaggtgcgca ttcttagatt gcattctgtt ttcagcattc cacacattca
     3781 ccaggttcat ttcttcttcc tggctgaaat tctagacggt cgatttggcg ctggaaaaga
     3841 aaccctggag gcaaagctcg tcacagcaga agagattccg tgggatgaaa tagcgttcgg
     3901 gtccgttcga tttgctctca aaagatacct ggatgccccg catcaaatgg aaacgcacgt
     3961 agattcttac ctcaaaccac agtgaaaatt cattgcccgc tgtgcgaaat catcgtctaa
     4021 cagttcatca aacctttggg agtccctcga tgaaaaaact cactgcagcc acaatgctcg
     4081 cggttaccct ggcagttgga tttgcactcg gcacgctgag cgcagaaaac cagccacata
     4141 tgaaagcagc cctgagccag cttgggcagg caaaagactc gcttcaaaag gcaactgctg
     4201 acaagggcgg acatcgcgca aaagcgattt ctctggtcaa tgaggcgatt gaggaagtga
     4261 agaagggaat tgcagcggac aacgcacgct gagcccgaac gaggggagag gaaactctcc
     4321 ccggcttttt acaagattac tttttggctt tgagttcagc ctgacgaccg acaagctctt
     4381 tggcgtattt gctattcatc cccttcatat gttcctgcgt ctttttaata gccgcgtcca
     4441 cttctgaaag ggtcatcctg ttgactttct tgttcttttt ctcgtctttt tgttcgtctg
     4501 ccataatttt gcctgtcgcg tcagagaact tgagataggc catgaagaca agtagaattc
     4561 ccgcagtcct actgctagcc ctgagcgcct gcgcgcactc tccagggggc tactacttct
     4621 ggaaatcgga atggaaactg gaagaaaaga acctccccta cctgaaccaa tccagattcg
     4681 cctatttacg gatgttcgat gttgccctgg aaggcgggga agcaaagccc tccggaacca
     4741 ttcggtttgc gtccagatgg ccagaacacc tggaagccgt cccggttgta ttcatcacgc
     4801 aggaagcgat caaagcccag aacgattcgc aagttcctgt cctggcagaa cgcattcaca
     4861 ggctcatgcg caatctggaa caaagtggat ctctgcccga aatcagagaa tggcaaatag
     4921 actgtgactg gtccgcgaag actgaatcca gatactttac acttctcaag caattgaaac
     4981 gactcgaacc gcaaagaaga ttgtctgtta ccatccggct ccaccagctc tccacaagtg
     5041 taccgcctgc agaccgaggc atgctgatgc tctacggctt tgaaaatcca gcggactacc
     5101 gtcaggcaaa ttccatcttt gatgaaaaaa gagccgctcc ctatctgaga aagctcaaat
     5161 cttatccact gactctcgac ctggcactgc ctgtgttttc ctgggcagtt gtgttctatc
     5221 aaggatcgtt tcgccacttg attcgagaca acactgcatc cttgcggcaa gcagatggcc
     5281 ttacggattt gagtgcgccc tggtccagat cgcagtggtt tcgcgctgag aaaaatctct
     5341 ggataggacc agaccgaatt ccggccggag ctgaaattcg actcgaagaa actggcccgg
     5401 ccatagcaag gcgcgcgaaa agcctggtag acttatccgc tcctccattt gcatctgact
     5461 cccgcattgt cctgtttgac ttccactccg cccagaacta ttctgtgtcc gaaatcgagg
     5521 ctatcttttg aaatcaaaac tgattctatc tgttcttgca ttcgctctct catttccatc
     5581 ggcgattctt ccctgtggac cgtcttcggg cctcgcagat ccttttttgt tttccgaaat
     5641 cccatccagt gcgtatggaa gagtttgcat cgattctttg ctcatagata aatccagcat
     5701 gcctgttgcc tgcggcgcca tcactcgcga agaggaaaac gcaagagaat gggctgccta
     5761 cctggggatc actgccgcag aagcacgcaa gggagtgtat gaaaatccaa agcttccagc
     5821 attcactcgt gcgcttgcaa agaagcccgg cgcatccgaa tacctcgctc tggccagatc
     5881 cgtagaaaaa gtacaactca gcacggtagg atgggattac gactttgatc gggaaaaaga
     5941 aaagtcagat ctatctcaaa caaaatccat tttaaaggat ctgcaaaaaa aggcagctac
     6001 tacgaatgat ccattccttt ctccgagata caatttacaa aaagcaaaac tacttctgag
     6061 actggggaac tatagcgaaa ttctctccat ccctgtgcct gaaaatccag ggttcaccag
     6121 atctcagatt ctaagactgc gctcgggtgc aagccttcgc atgaaagaaa aagtgactgc
     6181 ggccattcaa accattctgg catacgaagc gtctccctat tcaggcgctg cagacgattt
     6241 cagatatttg cttctggaag aaaaagacta cagacagatc caatcgtcca atctgtctaa
     6301 aaaacaagtt gggatatttt actttctttc aggaatccac atgtcctcag gcggctcctt
     6361 ttctctggca gtagaagacc tggagaatct ttggaatgca gagccgggca caccgtatgc
     6421 agatccactc cttctcaaaa cgatcgctct catggaagaa gaatctttgc cggtgatcct
     6481 gtatcaccag gagccaccaa agcagaatag cggcatcgat tcgattcgca ttcgactgct
     6541 ctcctggatt gctggtaaat ttcctcagct ctatgcgcaa actaatgcga acggacttcc
     6601 ggactccacc acactcgccg ccaccagact tcgcaaattc ctcgagaaaa ataaatccaa
     6661 ttccaggaat cctgctctgt ggaatctggc agctgcctat gccgcctaca cggcaggaga
     6721 cagcgacgcc gcaagcgcag ccctctctgc aaaagaaaag aagccagcct acgaagtgcg
     6781 aagacaactc attctacttc tggccaggat ccagaaagag agagtgatca gcgaagaaac
     6841 agaaaatgca ttcgtaaacc tgcttccgct gctttctggt tcatcctccg ggatggcacg
     6901 ttattcgcca tttgatgaaa aaggtccgcc tgcagcgacg atgatgcttt atgaactgct
     6961 ctctgacaaa tacaagaagg ggggtgccgg acacaaagga atcattgcat cctatctggc
     7021 cggtgcattc gaatggtacg acaccgctcg cctcgatgca tcttcagaac cggatctcgt
     7081 gcggctccgc gactatctgg cacagccgcc cgttacaagc atggatcgtt tgatcaaatc
     7141 caaattccac ctgtcgcgtg ccgtcgtcat ggatcgcctc gcggctaagt atgctctgca
     7201 gggaaaatac aaggaagcgg agcaagaact tagagcagcc ggtcctccca aaaaaatcac
     7261 tctggaatac acaggtgatc tgggcccgtt tgacgcgacc tttgaagacg caagcaagaa
     7321 tgcaaagaac tggaatcgcc tcaccttctt ccaggaaatg ggcaaattgt ctgcagccat
     7381 tgatgcgaaa ccggctgatg cggaggcggc tcttctccac tatcgccgcg gagtgggagc
     7441 tctgagcgca agccattttg ggaagtggtg gattctcatg aaggattaca ggtccgtggg
     7501 tgacgtctat atgggacatg actgggagct catgcttcgc caggcaaaga atgattttga
     7561 agcggcggct cgcctcacga aggatccaga actgggtgcg aagagcacgt tcctatccgc
     7621 acttgcgtcc cagggcttga atcaaccacg agaatggacg gaggaaaaac aaaaactttc
     7681 cgcggcagaa gcagacagtt atcgcaagct gggaacaatt tacaaagaca cgcgattttt
     7741 caacaccatt ggctcttcct gctcagtgct aaacgatttc ttaggccatt gaacaagggc
     7801 gcctaacggc agaaagttca acgttcggca ccgtctggtt ttgcggaaag caatttgaat
     7861 tcacgcagaa cgagcattgc aagaacggca atcaacgtaa cgtccacgcc ggtgaggctg
     7921 taatagatcc cgctcacccc aaaataaacg ggcaggatca gcaccacagg tacaaagacc
     7981 aatatctgtc tggacactgc gaggatggtt gcggtcttgc cgtgtccgat tgcctggaag
     8041 aaagtgagtc cgttgaaaac tcccgccatc agaggcagag ttacaatgag tatgcgaaaa
     8101 tttagaacgt cagtgtccgt gatggccgtg tccggcagca tccatccaag gaccgtgcgc
     8161 gagaacattg tcaaaggaag ccagatcaat gccatgaaag cagtgccacc gatgctgaag
     8221 acacgcaggg cttctatcac tcgggcgtac tggcgagctc caaagttaat gccaatgaca
     8281 ggctggagag cgcgaacaag ccccataacc ggataaacac acagaaacag gatgcgaagt
     8341 cctgtcccca tcaaggcaac atccttctca gtaccgtgcg ccgcgatcaa acggaaaaca
     8401 aagatctgct gcaaaacacc cattccttgt aaaataaaac tggaaaggcc aatcgagata
     8461 attcgtttaa aaattttagg atcgtatgca aaaagagaca cgggttctct cacactcgac
     8521 ttaccggacc taaaatagac ggcgcctgca gctgcaagag caaccattgc tacgttagtc
     8581 gcaagtgctg agccgcgaat acccagaccg aatgtgcgga taaaaagagg attgagcgca
     8641 acatttacaa ctgtgctgac cgcaagaatc ttcatcgcgg tacgcattct cccttcagcg
     8701 cgtaccagca tgttcgctga gattgcgtaa actgtgactg gaaagaacaa ggaaagaacc
     8761 gaaaaatact gggcacatcg cggccacacc catgggaata cttgcaagag gaaaagcgag
     8821 agacaccccg gcaaccgcct gctctcccac aagctgccca atgaaaatta catcaacgaa
     8881 agtgttgaga gcatagaaag acatgccgac gattgccggc catgccagat ctaccatgag
     8941 gcgccagagg ccgcctctca gtatataatc ttctgtgtct ttaaaccctg acgacacgtc
     9001 agagattgat ctgccactcg ttgcggcggt ttctcttgtc gtagtacgat gcaatcaaca
     9061 tgtccatgct gtaaagcgcc gtgcgaagat ttagatagat caatcgaagg gcacgcagct
     9121 ggatttcagg agaagtagct tccagtgtga tggcaggagc gccaggagat ggcatggaga
     9181 agttgtgacg atgcgtttcg agttccacca tctgcacgtt tggataatac aatctcatca
     9241 acttgcgtgt ccactgatcc ggatgataga cgttacgcgt cattgagtcc atcacaagac
     9301 aggtcaattt ggacgagcca tctgcaggta gatcatacgt aagactgaga actttcttgg
     9361 agttcacata atccccttct ctccacgcgc cggggacatc ttccagaacg atttcctggt
     9421 agtattgaag acgcggatag agctgcatca aacgcttgtg cttgagttca acgtctgtca
     9481 ctgcaatctt cacgttctga atttcaatgg taagaaggcg ttcaatctct tcgcggtatt
     9541 gcttttcctg agggttattg ccgattgcgt tttctgacat aatcggacgg ccatccgacg
     9601 gaacctgtgc ggttctggtt gcaggattca agcaacgtct ggccttatcc tccgactctt
     9661 tcgccggctg cggcgcagtt tgcgttgttt ccgcgaagcg aggaaatgcc acacccagaa
     9721 gggcggcaac aaacgcagat ataaaggcac gtctcatggt cgatatctcc cgtatgtatg
     9781 tgtcggcccg actaccccac aataaaaagc ggccgaacaa aaaaacacaa ggacttaaag
     9841 aaatttttgg aaacgtcgaa gctagcccgt cgcaggcttg agattgcaga gacggatgac
     9901 agtgcgatag aaattagcca gaatcacaat gttctgactg ggattgaggc cgcaggagac
     9961 tgccaggatg gccactacac agttgttcac tgattcttca tagtagtgcg tatgggtcac
    10021 ttccccggga acgatgtaat cctccccata aagaccagca ttcacgtttt ccgggcactt
    10081 ggccatgttg atgatgattg cttcgcgcaa ccttccctgg gtttcctttc tggtaatcac
    10141 cttctgaccg cgaatcgcag tcagaccgtc ttcacccatc catgcacagg atgcagccaa
    10201 cacgaacgcg aaaaagggag ccagaagccg catttcttcc agactcggca agcacgcaag
    10261 gcctgccaca aatttacaaa ttctgcttta tggcaggcct tccaaaaaag tttcttaaaa
    10321 aagcgcatgt caccaaaaca gaagaaaccc accgcatccg cccccggggc caaaaagaaa
    10381 gttccagccg atctaccgaa agaggcgtgg tctgcggcgc gcattcgcaa gctgtggatg
    10441 gattttttca aaagcaagaa gcacatggtt ctgcccagcg cgagcctctt gccttccgga
    10501 gatccaacac tcctgttcaa cactgccgga atggtgccct tcaaagctta cttcgcagga
    10561 acagaaaagc cgccctcccc tcgagtggcc acgattcaaa aatgccttcg cacaacagat
    10621 cttgaatccg ttggaaagac agagagacac tgtacttttt ttgaaatgct cggcaatttc
    10681 agcttcggag attatttcaa acgcgaatcc attcagtggg cctatgagtt ttcactgaag
    10741 catttaaacc tcgatccaga aaagatctac attacagtct atcaagacga cgacgaagca
    10801 gaagaaatct ggcacaaaga agtcggcgtt cctatgaaca gaattcgcag acttggaaag
    10861 gacgcaaact ggtgggggcc tgctggagat tcaggagcct gcggaccatg ctcggaactc
    10921 tacctcgaca gaggagaaga acgctgcaat cgcaaaccag acgcgtcctg cggccctggt
    10981 tgttcgtgcg atcggttcat ggaatactgg aaccttgtgt tcaatcaatt tcaccaggac
    11041 acttctggaa aacttcatcc actccccatg actggaatag acaccggagc cgggcttgag
    11101 cgaatcacaa tgctcaaact ggaagaaaac tcagtctacg acacggatga aatgaaacaa
    11161 atcatccgct tcatagaagg actcacggaa acactgcgcg aagataagaa aaaagtcgaa
    11221 tacgatccaa ccaacaagca tacagcacct tcctttcgcg tactcacaga tcacacgcga
    11281 gcaaccacct tcgcactgtc agac
//
LOCUS       DAOQJL010000054        11157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_2974_length_11157_cov_4.584669, whole genome shotgun sequence.
ACCESSION   DAOQJL010000054 DAOQJL010000000
VERSION     DAOQJL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_11157_cov_4.584669"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PLG78_03870"
     CDS             <1..1485
                     /locus_tag="PLG78_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE22275.1"
                     /translation="AAEAAMIVGLFPSPVRYSPMNNIKLSLHKQEMVLDAMERDGFIK
                     PGEQPGILKYFKARYEITEKSPGKIGLYGASRDFRLNSAPAANDRVRQFLYENIPEEI
                     VREGGLTVRTTIDPIKQAAALDAVRRGIERVRADMINKARDKTFAETQSRRLNGTLIS
                     MDPHTGELRALVGGYGVVETGSSDRIWKMQRQPGSAIKGFLYASALNAGSIDFEAEVV
                     DEPVSFNGYVPKNSYGSYKGKMTLRQAVALSCNTIAVKTLHELGPGEFRDMLGRALEL
                     SYFDTRERFQSNLTLALGSGEVTPLELARIYGILLNEGTTVRPVLIRSVTDKSGTVLW
                     EGTASSGSRVLSAESSSQAIDLLRGVVDPEDEGTAGWIGQQKLKNPNYLPFPIAGKSG
                     TVQTVPEVLRKFPGLTGVHDAWFVGLVPGDVSVVWIGQDEGAPFPGGGSGSAGTIWAD
                     YANSGFRSVTGDFPPVKRKKPVPEGSDPNSIVPLEPAPPPPPAQ"
     gene            1663..2394
                     /locus_tag="PLG78_03875"
     CDS             1663..2394
                     /locus_tag="PLG78_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNE22276.1"
                     /translation="MLRFIFVFSLLATGCVSTSTHEREMKTRDDEVSRLKDDVVRLET
                     EKSQLVKDRTALQASVTDMQKALTEARERKAESDKRVADYRSMLSRFRKLIDSGKLKI
                     RMVDGRMVVVLASDVLFASGSADLSPQGKDAVVEVAKVFATMPDRRFQIEGHTDNVPV
                     RTLKYPSNWQLAAERAISVLEVMVQSGVTPDRVSAASFAETRPLKPNDTVEGRSSNRR
                     IEIVIVPDLSSLPGFDELNKLAEEK"
     gene            2395..3012
                     /locus_tag="PLG78_03880"
     CDS             2395..3012
                     /locus_tag="PLG78_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE22277.1"
                     /translation="MGVQERKEREKAQRRTSILDAARQIIKEKPYDSIIMEDIANRLE
                     LSRATLYLYFKNKEEIYATLLTLGMRELQAGYDKALKNPSQDPMENLVKCAFEFFTFY
                     MSNHHYFDLLVTNRDDLLRECRPEVRKEFEEAGGAVIKPIAQVYKSGMDSGKFQKQDP
                     DKMAFLLRAVAIGIAVGFREGNLKFPEDILIMRDLVLHGLLGKKS"
     gene            3067..4617
                     /locus_tag="PLG78_03885"
     CDS             3067..4617
                     /locus_tag="PLG78_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE22278.1"
                     /translation="MAKQYDLIVVGGGISGACILWDATLRGMNVLLLEKGDFASGTTQ
                     ATSKLIHGGLRYLKNLEFGLVRESLRERRLLARLAPHAVKPMPFFHPIYKGAKLGRFT
                     LGLALSLYDVLSFDRNRGIPRHLRLPHHRWLSRDEALQAEPGLHPEGLQGAYIYYDYQ
                     NIGPERVCSDFIFSAVERGATARNYAEVKAIQQTLDGYSVTIEDRLTSKKESFEAKSV
                     VNAAGPWADLVDRATSSENSRKIVRSMGIHLVTRRIAGDSTVVLHRKDGSHFFIIPWR
                     EFSLIGTTDSLYEGHPDKFTVPESEIQKLIEDVTEGLNAKLTRADVVSVYGGLRPLVE
                     ESVSVKDSYSASRKTEIEMHADKPGFFTVLGGKYTTSRHLAEKAVDQIAAFLGGTWKK
                     CSTHRTALRTGDFGTQVLLKQKLFLERKHDRKKIETLISRYGLIADRMLSEKNLGVAF
                     TLDNGEVYYKEELETILNTEMVKSLKDLLLRRTGIGTLGISSPLPFAEEHGHVLLTQP
                     EATLRKAG"
     gene            complement(4614..5900)
                     /locus_tag="PLG78_03890"
     CDS             complement(4614..5900)
                     /locus_tag="PLG78_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22279.1"
                     /translation="MKSAILAWILVCVCACSCLIVADIYYCADQSALIRDSLSILHGH
                     FVSSGPESRGGVHHPGSLYKLLIALLLAVSGESLRVFAFMHVIVLSSSAFFIIRTTRI
                     LSGSIWFSLATLILFLALPFSFWYAMIVWGPAANVTAMCASLYFAVEIAKGKTALTPL
                     LMICLSFLFQTHYGTIFPVVALGLTGLWNSRTDKKAIHRALIVGAVFTLPALIDFVRE
                     PQNLRMILASAQGTQSLSSVWEAMVELHTMNASWIWGLAFALSIVVSLIRRSKVPASG
                     LNPQVWICVCLALASSILSMFFIREKIDPYYLAFMPAITLALLSIFARRIPIVLSILC
                     LFALPAAVSNVNRYLNEAWNVCPLGEVQTVARALNGKMAETKGASIPFVLPSILREAG
                     ELHPAKGLLIEAPPGDSSSRDIFRGKAIRLLEQPAR"
     gene            complement(5902..6498)
                     /locus_tag="PLG78_03895"
     CDS             complement(5902..6498)
                     /locus_tag="PLG78_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE22280.1"
                     /translation="MRTVLYLLLALLVLECKKPPVVEEYRFVSASSGLRLRKTPDLKA
                     DKIATIGNGAKVRVVEKGEPVEVDGIRSNWLQIDFLGQKGWAFGGYLTRPTKKPDPQW
                     EKFMGTWRGKNECKGEHTHLLIHPDFTYEGWLLDTAGAACAGHAVTGTWSMELSSICL
                     VDPTRACYEYGKGGLLLANQQAAPALREGAELLMQLKK"
     gene            complement(6510..7412)
                     /locus_tag="PLG78_03900"
     CDS             complement(6510..7412)
                     /locus_tag="PLG78_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE22281.1"
                     /translation="MKFSRAYFNQLLSPRAPNVDDRRLVLAGLPVYIVAAMSVFLVYA
                     QARRLMFMDVPAFEKQLSGEKKDEKIYPVLVEQNYRPKPETDQYRALSDVSAEGKGFL
                     TLNPGFHALTPYDRLELSAASRGSQSASSDNSANAAEGQGGKQGGNTGSNAPGNIGDD
                     ANFRIPQNYRFRQDFALRYDGSSRLSLARKELAGFRYFQKMIRQIEENFSPPGLNYAY
                     RDNAGLVTSEPVIPQSVQVLFMLDPEGNVRDVRKVSSIGQKAVDDACVNVLKGQNFGV
                     PPPEIFAQGNIFGINFIFPPVVNY"
     gene            complement(7387..8418)
                     /gene="ruvB"
                     /locus_tag="PLG78_03905"
     CDS             complement(7387..8418)
                     /gene="ruvB"
                     /locus_tag="PLG78_03905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNE22282.1"
                     /translation="MSVPEANKDFDEFSHDEAPEEKALRPGRLDEFVGQPLLKDRLSV
                     SIRAALHRKEPLDHVLLSGPPGLGKTTLACIVAAEMGGRFHSTSAPALGRPGDLARLL
                     TLLEKDDVLFIDEIHRLTRQCEEILYPAMEDGFIDFIVGEGMAAQSVKVPLKPFTLVG
                     ATTRSGSLTAPLKSRFGIDLKLEFYDLPELTKIVKRSAGLLGVQISENAAAVIAERAR
                     MTPRAANRLLRRIRDYATVENVNIIEPDFALRALGKLGIDSLGLTDLDRKLLTLVIER
                     YHGGPVGIRTLASLLDEEEHTIEEDHEPFMLRIGLLEKTPQGRVSTQKAAIHLGLKIV
                     PNEIQQSLF"
     gene            complement(8426..9538)
                     /locus_tag="PLG78_03910"
     CDS             complement(8426..9538)
                     /locus_tag="PLG78_03910"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNE22283.1"
                     /translation="MDRLEEKGRLRSLRVAQKGLDFASNDYLSLNSSGRLLSLMQKAA
                     TDWDGVVGSTGSRLLRGHHESFEIAEEEFATFCGTESSLLFHSGYAANVGVIQQFISS
                     EDTVFCDRLCHATILDGIRMSGARRFYFHHNDLDHLRDIMRKRERKGRSWIITESIFS
                     MDGDSPDLHSLVDLAEEEDALLYLDEAHALGVVGSTGAGLAHSLGLSRRVAVLVFPCG
                     KGPGLMGAFVCGSKMLRESMINSCRSFIFSTAQPPCLAHLLSLVFREISGMNVERKHL
                     SFLSQQLREGLHSMNLDFGKSTSHIVPVILGSEEDAMRWMNSLALRGIDARAIRPPSV
                     PEGSSRIRVNLHAGHSVTDVECLLKELYSLLPVGVR"
     gene            9669..10595
                     /locus_tag="PLG78_03915"
     CDS             9669..10595
                     /locus_tag="PLG78_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE22284.1"
                     /translation="MAAGIFTDDLFLQHDTGRSHPESPSRLLAIRERLEGGLASHFKT
                     LSRRFADPTQIQLIHDGRYVQELERFCNAHGGYLDGDTPVSDQSYRAALLAAGAGLEA
                     ADQIQSGNISRALLLVRPPGHHSLVQRAMGFCLFNNVAICARYLQLLGHKKIAIVDWD
                     VHHGNGTQDAFYEDPSVLFISLHQYPFYPGSGAASETGRGAGAGTTLNCPLPAGSADA
                     EYKQAFEKKILPALEQFGPNAILISAGFDAHRDDPLAGMLLTATSYEWMSRKLRDFAD
                     SHCDGRLISFLEGGYNLDALADSVEAHASVLI"
     gene            complement(10646..11122)
                     /locus_tag="PLG78_03920"
     CDS             complement(10646..11122)
                     /locus_tag="PLG78_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE22285.1"
                     /translation="MDIKDLLPWGRDKWSLDASFGSLQKEMNRIFDDFTRGLDTRSRS
                     FLSHFDPKINVAEDAKSIQVTAELPGMDEKDVDVSVNDYALTIKGEKKWEQESKEQDH
                     HLVERSYGSFRRVIPLPQGVDTSSIKATFSKGVLKVEVAKSQKVQESTRKIDVKAG"
BASE COUNT         2926 a         2925 c         2769 g         2537 t
ORIGIN      
        1 gctgcggaag cggccatgat cgtaggtttg ttcccgagcc ccgtacgata cagtcccatg
       61 aacaacatca aactatctct gcacaaacag gagatggttc tagatgcaat ggaacgtgac
      121 ggattcatca agccgggcga gcaaccagga attctgaagt acttcaaagc aagatatgaa
      181 atcacagaaa aatctccagg caaaattggt ctctatggag caagccgcga cttcagattg
      241 aattccgcgc cagccgcaaa cgatcgcgta cgccaatttc tgtacgaaaa tattccggaa
      301 gagatcgtgc gcgaaggtgg tttgacggtt cgcacaacca tcgatccaat caaacaggcc
      361 gcagccctcg atgcagtgag acgcgggatt gaaagagtgc gtgcggatat gatcaacaaa
      421 gcacgcgaca agacatttgc tgaaacacaa tcacgcagac tgaacggcac tttgatttcc
      481 atggatccac acacaggaga gctgcgcgca ctcgttggtg gatacggtgt agtggaaaca
      541 ggcagctcgg atcgaatctg gaaaatgcaa cgccagccag gaagcgcaat caaaggcttt
      601 ttgtacgcga gcgcactaaa cgcgggttcg atcgattttg aagcagaagt tgtcgatgag
      661 ccagtatcat tcaatgggta tgttccaaaa aattcatacg gatcttacaa aggcaagatg
      721 actctgagac aggcggttgc tctttcgtgc aatacgattg cggtgaaaac gcttcatgaa
      781 ctgggtccag gagaattcag agacatgctg ggccgggctc ttgagctcag ctactttgac
      841 acacgcgaac gctttcaatc caacctcact cttgccctgg gatccggaga agtcactccc
      901 ctggaactcg cgcgaatcta cgggattctt ctcaatgaag gaaccactgt tcgccctgtc
      961 ctcatccgca gtgtgactga caaaagcgga acggttcttt gggaaggaac cgcatcgtcc
     1021 ggatccagag tactgtccgc agaatcatcg tctcaggcaa tcgatctctt gcgcggagtg
     1081 gtagaccctg aagacgaggg aactgcagga tggatcggtc aacagaagct aaagaatccc
     1141 aactatttgc catttccaat tgcaggcaag tcgggaactg tccagacagt gccagaagtt
     1201 ctgcgcaaat ttccaggcct cacaggcgtg cacgatgcgt ggtttgtagg gctggttcct
     1261 ggagacgtat ctgtggtgtg gattggccag gacgaaggtg cgccgtttcc tggaggcgga
     1321 tcgggttctg caggaacgat ctgggcagac tatgcaaact ccggatttag atctgtgacg
     1381 ggtgatttcc caccggtaaa gcgaaagaag ccagtgcctg agggaagcga tccaaattcg
     1441 attgtgcctc ttgagccagc cccgccgcct ccgcctgcac agtgaggtct ccggtctttc
     1501 gaccggaggt tcttgaaacc ccggcttatc ttttttttcg ccgttaccct gtaacagaca
     1561 gtctgatcgg gcatgtcaag aaaaatcgac ccttgctgga aggcctggac tgcaaatcca
     1621 aataacgatt gcgcgcacat gtatttcggc cacttacagt caatgctccg tttcatcttt
     1681 gttttcagcc ttctggccac gggatgcgtt tccacctcca cccatgagcg ggaaatgaaa
     1741 acgagagacg atgaagtctc tcgcctcaag gacgacgtgg ttcgcctgga aacagaaaag
     1801 agccagcttg taaaagatcg cactgccctg caggccagcg tcacagatat gcaaaaggcg
     1861 cttacggaag cgcgcgaaag gaaagcggag tcagataaga gagtcgccga ctatcgttcc
     1921 atgctctcgc gctttcgcaa actcatcgat tctggcaaac tcaagatccg aatggtggac
     1981 ggaagaatgg ttgtagtcct tgcttcggat gttcttttcg cgtccggatc cgcggacctt
     2041 tcaccacagg gcaaagacgc agtcgtggaa gttgcaaaag tatttgcaac catgccggac
     2101 cgcagattcc aaattgaagg acatacggac aacgttccag ttcgcactct gaaatatcca
     2161 tccaactggc agctcgccgc ggaaagagcc atcagtgtgc tcgaagtcat ggttcaatcc
     2221 ggtgttacac cggaccgagt gagcgctgca agctttgcgg aaacaaggcc tcttaaacca
     2281 aacgacactg tagaaggcag atcatcgaat cgcagaattg aaattgtaat tgtgccggat
     2341 ctgtcgagcc ttccaggttt cgacgaactc aataagctag cagaagagaa gtaagtgggc
     2401 gtacaggaaa gaaaagaaag agaaaaggcg cagcgccgta catcgattct ggatgctgca
     2461 aggcaaatca tcaaagaaaa accgtatgac tccatcatca tggaggacat tgcgaatcgt
     2521 cttgagcttt cgcgcgcaac tctctatctc tatttcaaaa acaaagaaga gatctatgca
     2581 acgcttctca ctctggggat gcgcgaactc caggctggct acgacaaagc tctaaaaaat
     2641 ccttcccagg accccatgga gaacctggta aagtgcgcgt ttgaattctt cactttctac
     2701 atgtccaatc accattactt cgacctgctc gtcacaaata gagacgatct gttgcgcgag
     2761 tgcaggccgg aagtaagaaa agaattcgaa gaggcgggcg gcgctgtcat caagccaatc
     2821 gcccaggttt acaaaagcgg aatggacagc ggaaaattcc aaaagcagga cccggacaag
     2881 atggctttcc ttttgcgtgc cgtggccatt ggaattgcag tcggattccg cgaaggaaac
     2941 ctcaaattcc cggaagacat actgatcatg cgagacctgg tcctgcacgg actcctgggc
     3001 aagaaatcct gacagactgt cattttttgg cttgacgttc tgaccaaaca ctgacacggt
     3061 gtcagcatgg caaagcaata tgacctcata gtcgttggcg gtggtatttc gggcgcatgc
     3121 attctctggg atgcaactct gcgaggcatg aatgtgctcc tactcgagaa gggtgacttt
     3181 gcttctggaa cgacccaggc aacgagcaaa ctcatccacg gtgggctccg gtatttgaaa
     3241 aaccttgagt ttggtctggt tcgagagtcc ctgagagaaa ggcgtcttct ggcccgtctg
     3301 gccccacatg cggtcaagcc catgccattc ttccatccaa tctacaaagg tgcgaagctc
     3361 ggacgcttca cactcggtct tgcgctctca ttgtatgatg ttctctcctt tgatagaaac
     3421 cgggggattc cccgccatct gcgtcttccg caccacagat ggctctcccg tgatgaggcc
     3481 ctgcaggctg agcctggctt gcacccggaa ggcttgcagg gcgcttacat ctactacgac
     3541 tatcagaaca ttggaccgga aagggtttgc tctgatttca ttttctccgc cgtagaacga
     3601 ggagcgactg cgcgcaatta tgcagaagtc aaagcaattc agcagactct ggacggttac
     3661 agtgtaacaa tcgaagatcg cctaacgtct aaaaaagaat cattcgaagc aaaatctgta
     3721 gtaaatgctg cgggcccatg ggcagacctg gtggatcgcg cgacaagctc agaaaactct
     3781 cgcaagattg ttcgttctat gggaatccac ctggtcactc gccgcattgc aggcgattcc
     3841 acggtggttc tgcatagaaa agacggttct catttcttca tcattccctg gagagaattc
     3901 agtctgatcg gcactacaga ttccctgtac gaaggccatc cagacaagtt cactgttccg
     3961 gaatctgaaa ttcaaaagct cattgaggat gtaacggaag gactaaacgc aaaactcacg
     4021 cgcgcagatg tagtttccgt ctacggcggt ctccgtcctc tcgtagagga gtcggtttca
     4081 gtaaaggatt cgtattctgc atcgcgcaaa actgaaatcg aaatgcacgc agacaaaccg
     4141 gggttcttca cagtcctggg tggtaaatac accaccagtc gccatctggc ggaaaaagca
     4201 gtagatcaaa tcgctgcatt ccttggcgga acctggaaga aatgctctac gcaccgcacc
     4261 gctcttcgca caggcgattt tggcacccag gttttgctca aacaaaagct atttctggaa
     4321 cgcaaacacg atcgcaagaa aatcgagact ctgatttcca gatacggact gattgcagat
     4381 cgcatgctgt ccgaaaaaaa tctaggagtg gccttcaccc tggataacgg cgaagtctat
     4441 tacaaggaag agctcgaaac aattctgaat actgaaatgg taaaaagcct caaagatttg
     4501 cttcttcgtc gcactggaat tggaacactt ggaatctcaa gtcctctccc gttcgcagaa
     4561 gaacacgggc acgtactact aacacagcca gaagctactc tccgcaaagc cggctaacgg
     4621 gccggctgct cgagcagcct gatcgccttg cctcgaaaaa tgtcccgact ggaagaatcc
     4681 cccggagggg cttctatcaa gagccccttt gccggatgca gctctcctgc ctctcttaga
     4741 atggatggca agacaaacgg aatggatgct ccctttgtct ctgccatttt tccattcaga
     4801 gcacgcgcga ctgtctgcac ttccccgaga ggacatacgt tccacgcctc gttcaaatat
     4861 ctattcacgt tactcacggc cgccggcagt gcgaaaagac aaagtataga gagaacaatt
     4921 ggaattcgac gtgcgaaaat ggaaagcagt gcaagcgtga tggccggcat aaacgcgagg
     4981 tagtacggat caatcttctc gcggataaaa aacatggaca gaatggaaga ggccagagcc
     5041 aggcagacgc aaatccatac ctgtggattc aggccggatg ctggcacttt acttctgcga
     5101 atcaaggaaa ccacaatcga caaggcgaaa gcaaggcccc agatccacga agcattcatc
     5161 gtgtgcaact cgaccattgc ctcccataca gaagatagac tttgtgtccc ctgggccgac
     5221 gctagaatca tgcgaagatt ctggggttcg cgaacaaaat cgatcagggc aggaagagtg
     5281 aacacagcac ccacgatgag cgcacggtgg atcgccttct tatcggtacg cgaattccaa
     5341 agaccggtca agccaagcgc aaccaccgga aaaatcgtgc catagtgagt ttgaaacaga
     5401 aaactcaggc aaatcataag cagcggagta agggcagttt tccccttagc aatctccacg
     5461 gcgaaataca aagatgcaca catcgctgtt acgttcgccg ccggccccca tacaatcatc
     5521 gcgtaccaga aagaaaaagg aagcgcaaga aacagaatca gtgtggccag ggaaaaccag
     5581 atagatccgc tcaagatacg cgttgtgcga attataaaaa acgcggagga agaaaggact
     5641 atgacgtgca tgaaggcgaa gacgcgaagg gattcccccg acacagcgag caaaagggcg
     5701 atcagtagct tatagagtga gcctgggtga tgcacgccgc cgcgactttc tggacctgaa
     5761 gaaacaaaat gaccgtggag aatagacaga ctatcgcgaa tcagagcaga ctgatccgcg
     5821 caatagtaaa tatctgcaac tattaagcag gagcatgcgc aaacgcagac cagtatccag
     5881 gccaggattg cgcttttcat tttacttttt caactgcatc agtagttcgg cgccttctct
     5941 cagagcagga gccgcctgtt gattggccag aagcaggcca cctttcccgt actcataaca
     6001 agcgcgggtt gggtctacca gacaaatgga actcaactcc atgctccacg ttccagtcac
     6061 cgcatgcccg gcgcaggcag cgcccgccgt gtcgagcagc cagccttcgt atgtaaaatc
     6121 aggatgaatg agcagatggg tatgttcgcc tttgcattca tttttgccgc gccaggtgcc
     6181 catgaacttt tcccactgcg gatccggttt cttagttggt ctggtaaggt agcctccgaa
     6241 ggcccatcct ttctgtccaa ggaaatcaat ctgcaaccag ttgcttcgaa tcccgtccac
     6301 ctccaccggt tctccttttt ccaccacgcg aactttcgct ccgttgccga tggttgcaat
     6361 tttgtctgct ttgagatctg gagtttttct caagcgaagc ccggaggatg cggaaacaaa
     6421 acgatactct tccacaacgg gaggcttctt gcattcgaga acaagaagtg cgagtagcaa
     6481 atagagaaca gtgcgcatgg gcgcaaagtt caatagttca ctactggagg aaagataaaa
     6541 ttgatgccga agatgtttcc ctgagcgaag atttcaggcg gcgggactcc aaaattttgt
     6601 cccttcagca cgttgacgca cgcatcgtcg actgcctttt gtccaatact cgaaaccttt
     6661 cgcacatctc gcacgttgcc ttctggatcc agcataaaca aaacctgaac tgattgggga
     6721 atcacgggtt cacttgtcac aagtccggcg ttgtcgcggt atgcgtagtt gagtccagga
     6781 ggagaaaagt tttcttctat ctgacgaatc atcttttgaa aatagcgaaa gcctgcgagc
     6841 tcctttcgcg ccagagaaag ccgcgaagat ccatcgtatc tgagtgcgaa gtcctggcga
     6901 aatcgatagt tctgcgggat tctaaaattc gcatcatcgc ctatatttcc gggcgcgttg
     6961 gatccggtgt ttccgccctg ctttcctccc tgaccttctg cagcattcgc actgttatcc
     7021 gaggatgcgg actggcttcc gcgcgaagca gcgctcagtt ccaggcgatc gtacggagtg
     7081 agtgcgtgga atccagggtt gagtgtaaga aatcctttgc cttccgctga cacatctgat
     7141 agcgcacggt attgatctgt ttccggcttt ggacgatagt tctgttctac tagaaccgga
     7201 taaattttct catccttttt ttctccgctc agctgcttct cgaaagcggg aacgtccatg
     7261 aacatcaatc ttctggcctg cgcgtatact agaaacacag acatggccgc tacgatgtac
     7321 acaggcaagc cggccagcac aagtctacga tcgtctacgt tgggagcgcg tgggctcagc
     7381 aactggttaa aataagctct gctgaatttc attcggtacg atcttgagtc ccaggtgaat
     7441 cgcggccttt tgcgtactca ctctcccctg aggagttttt tccagtaaac caatgcgaag
     7501 catgaaaggc tcgtgatctt cttcgatggt gtgctcttct tcgtccagta gactggccag
     7561 tgtgcgaatg cctactggtc cgccgtgata tcgctcaatc acaagagtca aaagcttgcg
     7621 atcgagatct gtcagaccaa gagaatcgat tccaagtttg cccagtgcgc gaagagcaaa
     7681 atcaggttca atgatattca cattttccac ggtggcgtaa tctcgaatgc gacgcaaaag
     7741 tcggttggct gcccggggag tcattcgcgc tctttctgca atcactgccg ctgcattctc
     7801 ggagatttgt actccaagca agcctgctga tcgttttacg attttcgtca gctccggaag
     7861 gtcataaaat tcaagcttta gatcgattcc gaacctggat ttcaaaggag cggtcagtga
     7921 tccggaacga gtggtagcgc ctacgagcgt aaagggcttt aggggaacct tgacgctttg
     7981 agccgccatt ccttctccga cgatgaaatc aatgaaacca tcttccatcg caggataaag
     8041 aatctcttcg cactgtctgg tgagcctgtg tatctcatct ataaaaagaa catcatcttt
     8101 ctcaagaaga gtcagcagcc tggccagatc tcctggacgt ccgagcgcag gagcgcttgt
     8161 agaatgaaat ctcccgccca tctctgcggc aacaatgcat gcgagcgttg tcttgcctag
     8221 accgggtggt ccagaaagca aaacgtgatc gagaggttcc ttcctgtgaa gagcggcgcg
     8281 aatagaaaca gacagtctgt ctttgagaag aggctgccct acaaactcat cgagccgacc
     8341 aggccggaga gctttttctt ccggcgcctc atcatgcgaa aattcgtcga aatctttgtt
     8401 agcttccggc accgacatga tcgatttatc taaccccgac tggcagaaga ctgtaaagct
     8461 cttttaaaag acattccaca tctgtaacgc tgtggcctgc gtgtagattg acgcgaatgc
     8521 gagatgaacc ctctggcaca ctcggagggc gaatggctct cgcatctatt ccacggagtg
     8581 caagggaatt catccatctc attgcatcct cctccgagcc cagaatcacg ggaacaatgt
     8641 gcgatgtgct ttttccaaaa tctagattca tcgaatgcag tccttctcgc agttgttgcg
     8701 ataggaaaga caaatgtttc ctttctacat tcattccaga aatttcccgg aaaacaagag
     8761 aaagaagatg agcgaggcat ggcggctgcg cggttgagaa tatgaaggat ctacaagagt
     8821 tgatcatgct ctccctgagc attttcgaac cacaaacaaa agcgcccatc aaaccgggac
     8881 ctttgccgca cggaaaaaca agaacggcca cgcgacgaga cagccccaga gaatgcgcaa
     8941 gacccgcacc cgtggatcca acgacaccca gcgcatgcgc ttcatccaaa tagagcagag
     9001 catcttcttc ctccgccaga tccaccagcg aatggagatc gggactatct ccgtccatgc
     9061 tgaaaataga ctcagtgatg atccaggacc gacctttcct ttcgcgcttg cgcataatat
     9121 cgcgaaggtg atccagatca ttgtgatgaa agtagaaccg gcgggcgccg gacatccgaa
     9181 ttccatcgag aatggtcgcg tgacagagcc gatcgcagaa gactgtatcc tctgaggaaa
     9241 taaattgttg gatcacaccg acgttggctg catacccgct atgaaacaaa agagacgatt
     9301 ccgtaccgca aaaggtcgca aattcttcct ctgctatttc aaacgattca tgatggccgc
     9361 gcagaagacg cgaacctgtg cttcccacca ctccatccca gtcagtcgct gctttttgca
     9421 tcaaagacag caaacggccg cttgagttca gcgaaagata gtcattcgaa gcaaaatcaa
     9481 gccccttctg ggccacgcga agactgcgaa gtcgtccctt ttcttccaat cgatccaagc
     9541 cacgcctgag atcctggtga aattcgtcca gagcagtcat cgcaacaatt tcccgcagaa
     9601 acaggcacag gacgattctt tttcgattct tatcttgaaa tcaaacaaaa cgttggctac
     9661 catgacctat ggccgccggt atcttcacag acgatctgtt cttacaacac gacactggcc
     9721 gatctcatcc ggaaagcccg agtcgcctcc ttgcgatccg tgagagactc gaagggggcc
     9781 tcgcttctca cttcaaaact ctatcgcggc gtttcgcaga tccaacccag atccagttga
     9841 ttcatgacgg cagatacgta caagagctcg agagattctg caatgcgcac gggggatacc
     9901 tggacggaga cactccagtg tccgatcaat cttaccgggc cgcactgctc gctgccggcg
     9961 caggactgga agcagccgat caaatccagt ccgggaatat ttcgcgagcg ctcttgttgg
    10021 ttcgtcctcc cgggcatcat tctcttgtgc agagagctat gggattttgc ctcttcaaca
    10081 acgtggccat ttgcgcacgt tatctgcaat tgctcgggca caaaaaaatc gccattgtgg
    10141 actgggatgt gcatcatgga aacggaactc aagatgcgtt ttacgaagat ccctctgttt
    10201 tattcatcag tctccatcaa tatcctttct accctggatc aggtgcagcg tccgaaacag
    10261 ggagaggtgc cggcgcgggt acaactctaa actgtccttt gcccgccgga tccgcagacg
    10321 ccgaatacaa acaagctttt gaaaaaaaga tcctgcctgc tcttgaacag tttggaccca
    10381 atgcaatcct gatctcagca ggattcgatg ctcacagaga tgaccctctg gctggaatgc
    10441 ttttgaccgc aacgagctac gaatggatgt cgcgcaagct tcgagatttc gcagactccc
    10501 actgcgatgg tagactgatt tcttttctgg aaggtggata caacctggac gcgctggcag
    10561 attccgtgga ggctcacgcg tccgttctta tataagaatc tggcgaatga gaaggccgca
    10621 ggtcagaacc tgcggccccg aaacgttagc cggcttttac gtcgatcttc ctggttgatt
    10681 cctgcacttt ctgagacttc gcaacttcaa ccttgagaac gcctttagaa aacgtggcct
    10741 tgatcgagga agtatccacg ccctgtggga gtggaatcac cctgcgaaaa ctcccatacg
    10801 accgttccac caggtgatga tcctgttctt tgctctcctg ttcccatttc ttttcgcctt
    10861 taatagtcag cgcgtagtca tttacggaaa catccacatc cttttcgtcc atgccgggaa
    10921 gctctgcggt tacctgaatg gatttcgcat cttccgcgac attgatcttt gggtcaaagt
    10981 gcgaaagaaa gctgcgagat cttgtatcga gaccgcgcgt gaaatcatca aagatccgat
    11041 tcatctcctt ctgaagagaa ccaaacgacg catccaggga ccacttgtcc cttccccatg
    11101 gtaacaaatc tttaatatcc ataggaacct ccatttccta ctgcgaagat acaagag
//
LOCUS       DAOQJL010000055        10980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3053_length_10980_cov_4.914508, whole genome shotgun sequence.
ACCESSION   DAOQJL010000055 DAOQJL010000000
VERSION     DAOQJL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_10980_cov_4.914508"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLG78_03925"
     CDS             complement(<1..196)
                     /locus_tag="PLG78_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HNE22286.1"
                     /translation="MLDAHTIQIQRRALLEAEKLYAKPEFMASDPVELVHAYTDPLDQ
                     EVAALLCALFAYGNVKAMRGF"
     gene            complement(189..2621)
                     /locus_tag="PLG78_03930"
     CDS             complement(189..2621)
                     /locus_tag="PLG78_03930"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22287.1"
                     /translation="MNRIHYLVALTVLFVLYGIQFRETGFRLPIYYLPDGSIVSVRPA
                     DPPLRIQKILEIEGRVPHEAPATFRTDRAVLVYTDTGAAILKPEPVDRLQQFIDFAPI
                     ILLSALYLIAGIWFIQFGSDIYLSWFCVLASSFLYSFVAAIAGHGLDLLWQVFGFLLI
                     PAAFNLGLRTTGKEISGTLVIAEALVVLFFALIAYVGSDEVSTFGSLLLIQRGGFYVT
                     IVIVSGLQIENALRGSEDRIEKTKRWFLFSGTLIGLLLPAAAIEFRSGSGLDTLWFAG
                     ICASAFPVALVYGTYRIHVVPFQFVLGRSILAGLLTVFFVSLYAIVLLTHSLLLPNQD
                     QNYRWIVHLIFLLTLVFFLDPARQWMSAFIERNIWRLDSRLSESLKRIARIFSETSRI
                     DHAAEAFVDEIAATLTVRRCSLLISDRSFPGFRLKEDALIRMPDSSPLWEHIRPERLA
                     VAAYLAYAAGSRGILYRFLIESQFSMAAGVSGDEGPRFQAIIDKLLRRRRPVRKKGPY
                     VALLLGFRHDRSSFSLSEVRYIQEAARLARMLVSNFEILLAEVAKRRKMREIMLAGNT
                     QRSVTETWREADIRDLSIGAFSMPALSVTGDYVDLVPISPGRIAFFLGDVSGHGLGTG
                     YLVSAIRAMVRSHVQSGLGLSETMGTLNSFLMDRYRGNEFITLFAFYMDLEKGRLETL
                     NAAHPGPMVYRAATNTIETLRDTQRLPGLLPDPYHTSVLTMRRGDRLFLYSDGVTETF
                     SPASEPFGEARLRAFLYAHLGVNVSEIPDKLKRELTEHRQNPHCSDDTTFVAIEYAPR
                     QRGLFSFLNPDA"
     gene            complement(2618..3856)
                     /locus_tag="PLG78_03935"
     CDS             complement(2618..3856)
                     /locus_tag="PLG78_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE22288.1"
                     /translation="MIWPPGRIASFKWKTFGFSLLSGAVVVLIFILISGAVPGDIPHY
                     NEVVTLLFLLSSVLFLFPARDRLHKILFGKNESALFGDAHHLDFIARQFTVESLTMEV
                     FPSLMQWMGVRAAKLAIAEGGHSHYVYYVYSEGQPVRTRQSYYHSLEELLLYAKQERR
                     PLAPELPVPEEIRKLLTRIRAAYFLPIFYRNRMVGFLILHDPPDNKYADRALETFAGK
                     AAASIQNSLLSSRFMDATAMDREFLVAEKIRKFLQRTEIPQIPGFKIEAFTGERAPIV
                     IEFLRAPDRSDVWFAVLFAADRLNGSTAILLSSRLGSLYSLSKKESSLNLHKLLTHFR
                     RNRADGFDRVDLCIAEIRPHENAILCMIDGPGFAVRNPLTPEKTIVSPGWRNVVDMKE
                     NSTLQIEYAGQPLLLFAAQK"
     gene            complement(3853..5085)
                     /gene="argJ"
                     /locus_tag="PLG78_03940"
     CDS             complement(3853..5085)
                     /gene="argJ"
                     /locus_tag="PLG78_03940"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNE22289.1"
                     /translation="MSNFPRGFLAAGWNAGIKDNTLDFGVVLSERPASAAAIFTRNNF
                     PGNPVIVGREHIKGGVLQAVVVNSKNSNVGTGPAGLQLARDMCTLSAASLGIKPEHVL
                     PSSTGVIGRLPLRDKIEKACGEARSRLKTADFETFADAIRTTDKTVKTASASLNSGAR
                     IVITAKGAGMIEPNMATMLVYAFTDAEIASADLDRMLRVVADRSFNRITVDSDTSTSD
                     TFAILANGLSGVSISFPESASDAFKNSSVFMPDVATVPGLDENAREFVTEFLALSQAL
                     ARAIVQDGEGSTKILEVRITEARDKTQALKIARSIANSPLIKTALHGGDPNWGRILMA
                     VGKVFDEPVPFEQLKIYFGETLLRYGDSSQLPELEKYLKQSSVVLRISLGAGQAEEKI
                     WGCDLSREYISINADYTT"
     gene            complement(5116..5436)
                     /gene="cutA"
                     /locus_tag="PLG78_03945"
     CDS             complement(5116..5436)
                     /gene="cutA"
                     /locus_tag="PLG78_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNE22290.1"
                     /translation="MTPLFVYITTSGPEEARKIGTALVEMRLAACVNILPPVESIYHW
                     KGAIARETETVLIAKTRQDLFGRLSETVRSLHSYTNPCIVGLPIKEGSAEYLQWIADE
                     TASA"
     gene            5492..6250
                     /gene="fabG"
                     /locus_tag="PLG78_03950"
     CDS             5492..6250
                     /gene="fabG"
                     /locus_tag="PLG78_03950"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNE22291.1"
                     /translation="MEKPLKGKIALVTGSARGIGRAIAEDLAEWGADQVILDMNQEAC
                     EKTAAEIAGKYGVRAIAVACNITKKSDVEAAFSKIQKEYGTLHFLVNNAGVLKDNLLL
                     RMKEEEWDMVLGVNLKGAFLMTQVMLKMLMKAEGGSRIVNISSVSGLAGQIGQANYSS
                     SKAGLIGLTKVTAREYGSRNVLCNAVCPGYVQTDMTGALPPEVQEMLRKSVPLGRPGQ
                     VKDISRVVRFLCSDDASFISGTIIRVDGGMATGM"
     gene            complement(6264..7472)
                     /locus_tag="PLG78_03955"
     CDS             complement(6264..7472)
                     /locus_tag="PLG78_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22292.1"
                     /translation="MYFLGLFTSGLQALPLLPDASCDNHGGMEARSSHDSRAGLTVRM
                     ECFGWQLAGSLSGRDHSTTGDSIIGSIRAESNVATIIAGRRDLPALHSGYLTDEVSVL
                     VSPLRKSSVFVRPVPSLWVPGIFAVEGMTGPGIFFLSPDGRVFAALHPDGSCGLTVDY
                     QTGARVFVDAERLRFGEKLPWTWEGVAYASFGGVNIETSRQRKWDFDEKGRFDESRKG
                     RSGLASIVLGPDWLHVEAAGMDRYKTSMRMAGATAACIPDILSFGPVIRGRSYRVRTY
                     GSSSEVTDSAFAGGLALQTQFVQLEALYESRRGAASSGELSLSIERKNLSASLSGFAK
                     CGRKIEGRSLRVLRTRPEYETGTTFYLEETSALTLRVRSGFLYLSAGFSRSKNKTNRY
                     GTAEFKSEFF"
     gene            7591..8091
                     /locus_tag="PLG78_03960"
     CDS             7591..8091
                     /locus_tag="PLG78_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE22293.1"
                     /translation="MSDRLRDLYSANRRDLYTYLSRMSGDADLALDLLQESFLNYFEH
                     YRGKEMPDDLSSRRILFRIARNLFINHFRSKASQTTHLEEQYLEAAPAQNAGPDLTFA
                     IQKLIQGLPEKERTAIVMRYLESMRLEDIGAVLGLSVSAVSRLVSKSEKMLEWEAKKA
                     GISLDA"
     gene            8172..9161
                     /locus_tag="PLG78_03965"
     CDS             8172..9161
                     /locus_tag="PLG78_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE22294.1"
                     /translation="MENKRSSLARALNTSDALPDFNPEWLTRSPRLRADASVFAERTN
                     RMPYYAAAAAVLFALLGTSLYLRSPAPAAQVASRGLVISVAGPTQVQRGEATTDLYNG
                     DMITEGDRITTGKDARVEIALAGHSVRILENSSVTLLELKKTSEGQRNVGLDVAQGTL
                     LSSVKRLSKQESFEVRTPVAIAGVRGTRFSVAVNDSGSQVRLYDGAVVVKRGESETVL
                     SPGKGVEVTKSGVSELTEDSTDYAQQIQEMETNPNADPEIIAAADSLKSAKSEKELKA
                     LYQKIEIIRLRDGRVIRGVIAQQVGSRVVIHTSTGMHVVQSSEIEDQSFAEEP"
     gene            9212..10315
                     /locus_tag="PLG78_03970"
     CDS             9212..10315
                     /locus_tag="PLG78_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE22295.1"
                     /translation="MKTRIKFLAIALIASAATLAVNCGQKASEAANTSMMVVYTTGSA
                     FILPEGKSEVPAKVGMVVRQNDVIRTESGTVDLQTRSGSAVRVREMTRITISALNASG
                     ETKLNMQHGGLLASVKKAGQGENFSVVTPTAIAGVRGTTFSVEVGEDSRSSVRVIDGK
                     VAMAPRVASLDKFSDAQIKADPNLQKLSDLSKKEIVLEDKTQGTLDANLEKQLMNANQ
                     VLDSASPAPAKLTDVVAATSTAKPVESQKVEVSTREIIEARTLVTVDPSIMEKAVKGQ
                     ESEAAKEITSRREVAQETVLNQIMEDAGSVKLNSEKEIQQRYNKLELVVLKNGDQIRG
                     AVIAQTENSIVVHSPEGVKKIPRSDLNYQEPLF"
     gene            10429..10623
                     /locus_tag="PLG78_03975"
     CDS             10429..10623
                     /locus_tag="PLG78_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNE22296.1"
                     /translation="MRPRKVCVCRSVTETEIRESVRNGHHTFEAVQEETDCSTGCGTC
                     ESQVRSIIANELSALQAAKK"
     gene            10660..>10980
                     /locus_tag="PLG78_03980"
     CDS             10660..>10980
                     /locus_tag="PLG78_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22297.1"
                     /translation="MFSLQWSDLIRAGGPTLFVLLICFIATCILIIERSAYFRARARE
                     TAAVLEQLRAQEGSTVLPRDSYKMVNSPVTSVLYDCYPYAGGSLFEEVKSRAIAENLP
                     SIERY"
BASE COUNT         2792 a         2724 c         2851 g         2613 t
ORIGIN      
        1 gaaaacctcg cattgctttt acgtttccat aggcgaacaa agcgcaaagt agggctgcaa
       61 cttcttgatc cagcggatct gtgtaggcgt gaactaactc aactggatca cttgccatga
      121 attcaggttt cgcgtacagt ttctccgctt ccaggagggc gcgcctttga atctgaatcg
      181 tgtgtgcgtc aagcatccgg attcagaaaa gagaaaaggc ctctctggcg aggtgcgtat
      241 tctattgcca cgaacgtagt gtcgtcgctg cagtgtggat tctgtctgtg ctctgtgagc
      301 tctcttttga gtttgtccgg gatctcactt acatttacac caagatgtgc gtagagaaaa
      361 gcgcgaagcc tcgcttcccc gaatggttca gatgcagggc tgaatgtttc tgtcactcca
      421 tctgaataca aaaataagcg gtctccccgt ctcatggtca atacagaagt gtgatacgga
      481 tccggtagta gccccggaag gcgttgcgta tctctaagtg tttcaatcgt gtttgtagcc
      541 gcgcgataca ccatggggcc cggatgcgca gcattcaaag tttccagtct tcctttttcc
      601 agatccatat aaaaggcgaa taacgttatg aattcatttc cacggtatcg atccataagg
      661 aagctgttga gagttcccat ggtttcagaa agaccaagac cactctgcac gtgcgatctt
      721 accatggcgc ggatggcact taccaggtat ccagttccaa ggccgtggcc gcttacatcg
      781 cccagaaaga atgcgattct gcccggcgaa atcggcacca ggtccacgta gtctccggtg
      841 acggaaagcg ctggcatgct gaatgcgcca atgctcaaat cacggatgtc tgcttcgcgc
      901 caggtttccg ttacgcttct ctgcgtgttt cctgccagca tgatctcgcg catcttcctt
      961 ctttttgcaa cttctgcgag gaggatctca aagttagaca cgagcatgcg cgcaagtctg
     1021 gccgcttcct ggatgtatcg cacttcagac aggctgaagc tggatcgatc gtgccggaag
     1081 cccagcaata gagcaacata tggccccttc tttcgcacag gtcttcgcct gcgcagtagt
     1141 ttgtctatga tcgcctggaa gcgaggccct tcgtctccag aaactccggc cgccatagag
     1201 aattgcgatt ctatcaggaa tcgatagagt attcctcgac ttccagcggc gtaagcaaga
     1261 tacgcggcga ccgccagacg ttccggtcga atgtgttccc agagcgggct tgagtctggc
     1321 attcgtatga gtgcgtcttc ttttaatcgg aaaccgggaa aggatctatc ggatatcaac
     1381 aaagaacagc gtctaacggt gagtgttgcc gcgatttcat ctacaaacgc ttccgcggca
     1441 tggtcaatgc gacttgtctc agagaagatt cttgcgattc tcttgagtga ttctgaaagg
     1501 cgagagtcca gtctccagat gtttctttct atgaatgcgc tcatccactg ccgcgcgggg
     1561 tccaggaaaa agactagggt cagaaggaag atcaaatgaa caatccatct gtagttttga
     1621 tcctggttgg gtaaaagaag ggagtgagtg agaagtacga tcgcataaag agaaacaaaa
     1681 aaaacagtca gcagtcccgc aagaatggaa cgtcccaaga caaactggaa aggcaccaca
     1741 tgaatgcgat aggtgccgta tacaagtgcc accgggaatg cagaggcgca aatgcctgca
     1801 aaccatagag tatcgagacc agacccggat ctaaactcaa tggcagcagc cggaagtaga
     1861 agtccaatga gcgttccaga aaacaaaaac catctttttg ttttctcaat tctgtcttcc
     1921 gagccgcgca gtgcgttttc tatttgcagg ccagagacga tgacaatcgt aacatagaat
     1981 ccacctctct gaatgagaag gagactccca aacgtgctca cttcatctga tccaacatag
     2041 gcaatcaaag caaagaatag aacgaccaga gcctctgcga tcacgagcgt tccactgatt
     2101 tcctttccag tcgtgcgaag tcccaggttg aatgcggcgg ggatgagtaa aaatccaaac
     2161 acctgccaga gtagatccag tccatggccg gcgatggccg ccacaaatga atagaggaag
     2221 gaagatgcca gaacgcagaa ccaggaaaga tagatgtcgc ttccaaattg aatgaaccag
     2281 attcctgcaa tgagatagag cgctgagagt aagatgatgg gcgcaaaatc tatgaattgc
     2341 tgcagccgat cgacaggttc tggcttgagt atggcagcgc ctgtgtccgt atatacaagc
     2401 actgctctgt ctgtgcgaaa agtggccgga gcttcgtgcg gcactctacc ttctatctca
     2461 aggatttttt ggatgcgaag aggaggatcc gcaggacgaa cagaaacgat ggatccatct
     2521 ggcaaatagt agatgggcag gcgaaatcct gtttcgcgaa attgaattcc gtagagaaca
     2581 aatagaacgg tgagcgcgac caggtagtgg attctgttca tttctgcgcc gcaaacagga
     2641 gcaatggctg tcctgcgtat tcaatttgaa gggttgagtt ttccttcata tctaccacgt
     2701 ttctccatcc aggggaaaca atggtctttt ccggggtcag agggtttcgc acagcgaatc
     2761 cggggccgtc aatcatgcag aggattgcat tttcatgcgg tctgatttct gcgatgcaaa
     2821 gatccactcg atcaaatccg tccgcgcgat ttcgacggaa gtgagtcagc agtttgtgca
     2881 gattgaggct ggactctttc ttggagaggg aatacaggct gccaagccga cttgagagca
     2941 gaattgcggt ggatccgttc agtctgtctg ccgcaaacag cacggcgaac catacatcac
     3001 ttctatctgg cgcgcgtagg aactcaatga cgataggcgc tctttctcct gtgaatgctt
     3061 ctatcttgaa gcccgggatc tgtggtattt ccgtgcgctg aaggaacttt cgaatttttt
     3121 cagcaaccag gaattcccgg tccatcgcag ttgcgtccat gaacctggag gagagcagag
     3181 agttctggat ggaggctgcg gctttccctg caaatgtttc gagtgctcta tccgcgtact
     3241 tgttgtctgg tggatcgtga agaatcaaga atcctaccat tctatttcta tagaagatag
     3301 gaagaaagta agctgcgcga atccgagtga gtagctttcg aatttcttct ggcactggaa
     3361 gctcgggtgc aagtggtctg cgttcctgtt ttgcgtagag caaaagctct tcgagcgaat
     3421 gatagtacga ctggcgcgtt cgaacgggct gaccttctga atatacgtag tacacataat
     3481 ggctgtgtcc accctctgcg atggcgagtt ttgcagcccg cactcccatc cactgcatga
     3541 gagacggaaa tacttccatg gtcagtgatt ctacggtgaa ctgccgcgcg atgaaatcca
     3601 gatgatgcgc gtctccgaag agagcggatt cgtttttgcc aaacaagatt ttgtgcagtc
     3661 gatctctggc agggaataga aagagaacgg aggaaagcag aaacaataga gtgaccactt
     3721 cattgtagtg aggaatgtcc ccaggaacag ctcctgagat cagaatgaaa atcagaacta
     3781 cgaccgctcc ggagagcaag gaaaacccga aagtcttcca tttaaacgaa gcgatgcgcc
     3841 ccggcggcca gatcatgttg tgtagtctgc gttgatgctg atgtattctc tagaaagatc
     3901 acagccccag attttttctt ctgcctggcc tgcacccagg gagatccgca gcaccacaga
     3961 gctctgcttc aggtattttt caagctcagg caactgcgaa gaatcgccat agcgcaacaa
     4021 tgtctccccg aaataaatct tcagctgttc aaatggaact ggttcatcga ataccttccc
     4081 cacagccatg agaattcttc cccagtttgg atctccacca tgcaaagctg ttttgatcag
     4141 cgggctgttt gcaatggaac gagcgatctt gagcgcctgg gttttgtctc ttgcttctgt
     4201 gattcgcact tccaggattt ttgtggatcc ttctccgtcc tgaacaatcg cgcgggccag
     4261 tgcctgcgaa agtgcaagga actcggttac gaattctcgc gcgttttcat ccagtccagg
     4321 aacggttgcg acgtccggca tgaagactga tgagtttttg aatgcatctg aggcagactc
     4381 cggaaacgaa atcgaaacgc cggacaatcc atttgcaaga atcgcaaatg tatctgacgt
     4441 ggacgtatct gaatctaccg ttatgcgatt aaaggaacga tctgcgacta cacgcagcat
     4501 tctgtccaga tctgcggaag caatttctgc atctgtgaac gcatagacaa gcatggtagc
     4561 catgtttggc tcaatcatcc ctgctccctt ggccgtgatt acaatgcgcg ctcctgagtt
     4621 cagggaagcg cttgcagtct ttactgtttt gtctgttgtg cgaattgcgt ctgcgaatgt
     4681 ctcaaagtct gcagtcttca ggcgcgacct ggcttccccg catgcttttt cgattttgtc
     4741 gcgcagtggc agcctgccta tgactccagt gctcgatgga agaacatgct ccggcttaat
     4801 tccaagtgac gccgcagaaa gagtgcacat atcgcgcgcc agctgaagac ctgccggccc
     4861 tgttcccacg tttgaattct ttgagtttac aacgactgcc tgcaacactc cgcctttgat
     4921 atgctcgcgc cccacaatga ccgggtttcc aggaaagtta tttcgcgtaa atatcgcagc
     4981 cgcggaagca ggcctttcgc tcagcaccac tccgaagtcc agtgtgttgt ctttgattcc
     5041 ggcattccag ccggcggcaa gaaagccgcg tggaaaattg ctcacaggaa acagaagtct
     5101 gcgccggaca gcatgtcaag cggatgcggt ttcatccgca atccactgca gatattctgc
     5161 ggacccttcc ttgatcggaa gacctacaat gcaggggttt gtgtagctat gaagggaccg
     5221 caccgtttcg ctcaatcgcc cgaaaagatc ctgacgagtc tttgcgataa ggactgtttc
     5281 tgtttcgcgt gcgatcgcac ccttccagtg atagatcgac tccacaggtg gcagtatgtt
     5341 cacgcaggct gccaggcgca tctccacaag ggctgtgccg atttttctcg cttcctctgg
     5401 tcccgatgtt gtgatgtaaa caaagagcgg tgtcatctgc tgattcgaat atcgcttgat
     5461 acacaggcaa cctggattcg atctgctttc tatggaaaaa ccactcaaag gtaagattgc
     5521 tctggtcaca ggatctgcgc gcgggattgg ccgtgctatc gcagaagatc tggcagaatg
     5581 gggcgcagat caagtcatcc tggatatgaa ccaggaagca tgcgaaaaga ctgctgcgga
     5641 aatcgcaggt aagtatggcg tgcgagcgat cgccgttgct tgcaacatca caaagaagtc
     5701 agatgtggaa gcagctttct cgaagattca aaaagagtat ggcacacttc attttcttgt
     5761 gaacaacgca ggcgtactca aagacaatct gcttcttcgc atgaaggaag aagaatggga
     5821 tatggtactt ggcgtgaacc tgaagggagc atttctgatg actcaggtga tgctcaagat
     5881 gctcatgaag gcagaaggcg gatcacgcat cgtcaatatc agttctgtct ctgggctggc
     5941 gggtcagatt ggccaggcca actattcttc gagcaaagca ggtctcattg gtctgacaaa
     6001 ggtgactgcg cgtgagtatg gttcgcgcaa tgtgctgtgc aatgctgttt gtcctggtta
     6061 tgtgcaaaca gacatgacag gcgctttgcc gccagaagta caggaaatgc tgcgcaagtc
     6121 agttccactg ggaaggccag gtcaggtgaa agacatttct cgcgtggttc gttttctatg
     6181 ttcagatgat gccagtttca tcagcggaac gatcatacgc gtagacggcg gcatggccac
     6241 tggcatgtaa gagaatctcg aattcaaaaa aattctgatt tgaactcagc cgttccgtag
     6301 cggttggttt tatttttgga acgagaaaac ccggcgctca agtacagaaa tccgcttctc
     6361 actctcagag tgagcgcgga tgtttcttct aaatagaagg tggtgcctgt ttcgtattct
     6421 ggtctggtcc gaagcacccg tagacttctc ccttctatct ttcgtccgca ttttgcaaaa
     6481 cccgacaggc tagcgctcag gttttttctt tctatagaaa gactcagttc tccagatgaa
     6541 gcggctcctc ttcgcgattc gtaaagtgct tccaattgaa cgaactgcgt ttgcaaggcc
     6601 agtccgccgg caaatgctga gtctgtgact tcagacgacg atccatacgt tctgactcta
     6661 taggatcttc cccgaatcac tggaccaaat gacagaatgt ccggaatgca tgcggcggtt
     6721 gctcctgcca ttctcatgga tgttttgtaa cgatccattc ctgcagcttc cacgtgcaac
     6781 cagtccggtc caagcacaat cgacgcgagt ccggatcttc ctttgcgcga ttcatcgaac
     6841 ctgcctttct catcgaaatc ccatttcctc tgacgcgatg tttctatgtt cactccacca
     6901 aacgacgcgt atgcgacgcc ttcccaggtc cacggcagtt tttccccgaa gcgcaacctc
     6961 tccgcgtcca caaagacgcg cgctcccgtc tggtagtcta cagtgagccc acaactacca
     7021 tccgggtgaa gcgcggcaaa cacgcgcccg tctggactga gaaagaatat gcctggtccg
     7081 gtcatccctt cgactgcgaa gattccgggc acccacaggg aaggaaccgg tcgaacaaag
     7141 acggatgatt ttctcagagg tgagacaaga acagacacct cgtctgtaag atagcccgaa
     7201 tgaagagcgg gcagatctcg tcttccggct atgatggtgg ccacgttgga ttctgcccgg
     7261 atcgatccta tgatggagtc cccggtggta gagtgatcgc gaccgctgag cgatcctgcc
     7321 agctgccagc cgaaacattc catgcggacg gtcaggccgg ccctcgagtc gtggctcgat
     7381 cgcgcttcca ttccgccgtg gttgtcgcag cttgcatccg gaaggagggg aagcgcctgg
     7441 aggccagacg taaaaagccc gaggaaatac accagaacag gaaaatgctt tccagcctgc
     7501 ttcccggggc aattgtgcgg accgtgaccc atcgcaatcc agtaggtagg ccattcaaga
     7561 attgttccgg cttatttcga gtaggcaatc ttgtctgatc gtctgcgtga cctctattct
     7621 gcgaatcggc gggatctata cacgtatctg agccgcatgt caggcgacgc agatctggcc
     7681 ctggatctac tccaggagtc gttcctcaac tatttcgagc actatcgcgg caaagaaatg
     7741 ccggacgact tgtccagcag gcgaattcta tttcgcatcg cgcgaaatct attcatcaat
     7801 cattttagaa gcaaggcaag ccagaccact catctggaag aacagtatct ggaagccgca
     7861 cctgcgcaga atgctggacc cgaccttaca tttgctattc aaaagctcat ccagggcctt
     7921 ccggaaaagg aacgcacagc gatcgttatg cgatatctag aatccatgcg attggaagac
     7981 attggcgcag ttctcgggct ttctgtttcc gccgtatcca gactcgtaag caagtctgag
     8041 aagatgctcg aatgggaggc caaaaaggcc gggatctctc tggatgcgta actggacgcg
     8101 aaaatcatcg cttttgagac aaaaaaagag caaaaaaatt ctgacacgtt gttagagtag
     8161 ggatgggaga catggagaac aaacggtcca gtctggctcg cgcgctaaat actagcgatg
     8221 ctctcccaga ctttaatcca gagtggctca ccaggtcgcc acgtcttcgc gcagatgcat
     8281 ctgtcttcgc agagcgcacc aatcgcatgc catactacgc ggccgcagcc gctgtcctct
     8341 ttgccttgct aggtacttct ctttatcttc gctctcctgc tccggccgcc caggtggctt
     8401 ccagagggtt agtgattagt gtggccggtc ctactcaagt gcaaagggga gaggccacca
     8461 cggacttata caatggagat atgatcacgg aaggggatcg catcacgaca ggtaaagatg
     8521 cgagagtaga aattgcactc gcaggtcatt cagttcgcat tcttgaaaat agttctgtaa
     8581 cccttcttga actcaagaag acatcggaag gccagcgtaa cgtcggattg gatgtagcgc
     8641 agggaactct actgagctca gtgaaacgat tgagcaaaca ggagtccttc gaagtgcgca
     8701 caccggtcgc cattgcgggc gtgcgcggca ccaggttctc tgtggctgta aacgattctg
     8761 gttctcaggt cagactttat gacggcgctg tagttgtgaa gcgtggagaa tccgagaccg
     8821 ttctttctcc agggaaagga gtggaagtca ccaagtcagg tgtgtcggaa ctgacagagg
     8881 attccactga ctatgcgcag caaattcagg aaatggaaac aaatccaaat gcggatcctg
     8941 aaatcattgc agctgcagat tctctcaagt ctgcgaagtc tgaaaaagaa ttgaaagcat
     9001 tgtaccaaaa gatcgagatc atacgtctaa gagacgggag agtcatccgc ggtgtgattg
     9061 cgcagcaagt cggatccagg gtggtcatcc atacttctac gggaatgcat gtagtgcaat
     9121 catcggaaat tgaagaccag agtttcgcgg aggaaccatg aacagtatta tagttaaaac
     9181 gccggtatgt ccggtaaaag gagacccaac aatgaagacc cgaatcaagt ttctagccat
     9241 cgcgctgatc gcatctgcgg caacgctggc agttaactgc gggcagaagg cgtctgaggc
     9301 ggccaacact tccatgatgg ttgtgtatac caccggaagc gcatttattc tgcccgaagg
     9361 aaagtccgaa gttccggcca aggtaggaat ggttgttcgt cagaatgacg ttattcgtac
     9421 cgaatcggga actgtggacc tccagacaag aagcggaagc gcagtgcgcg tacgcgaaat
     9481 gacgagaatc acaatctccg ctctcaacgc gagcggcgaa actaagctga acatgcagca
     9541 tggcggtttg cttgcttctg taaagaaagc aggacaggga gaaaacttct ccgttgtaac
     9601 accgaccgcg atcgctggtg tgcgcggaac aactttctca gttgaagtag gcgaagacag
     9661 ccgttcttcc gttcgagtaa tcgacggtaa agtggccatg gctcctcgcg tagcttctct
     9721 ggacaagttc agcgatgcgc aaatcaaagc tgacccgaat ctccagaaac tgtctgacct
     9781 gagcaagaaa gaaattgttc tggaagacaa gactcaagga acgcttgatg cgaacctcga
     9841 aaagcagctg atgaacgcga atcaagttct tgattcagca tctccagctc ctgcaaaact
     9901 cacagacgtt gtggctgcta caagcaccgc aaagccagtt gaatcgcaga aagtagaagt
     9961 aagcacacgt gagatcattg aagctcgcac tctggtaaca gtggatccat ctatcatgga
    10021 aaaagctgta aaaggccagg aaagcgaagc tgcaaaagaa atcacatcca ggcgtgaagt
    10081 tgctcaggaa accgtactca accagatcat ggaagatgcg ggaagcgtga agctgaactc
    10141 tgaaaaagag atccagcagc gttacaacaa gcttgagctt gttgtcctca agaatggtga
    10201 ccagattcgc ggcgctgtga ttgctcagac tgagaattcc atcgtcgtgc attcaccaga
    10261 aggcgtgaag aagatcccaa gatcagacct gaactatcag gaaccactgt tctaagatca
    10321 gaattttcac gggagctgat tcgtcggctc ccaaaaaact tctcgaaaag cgccccccgg
    10381 ggcgcttttt ttgtttaaat ggacgatttg tcagcagtgt atgcaatgat gcggccgcgg
    10441 aaggtttgcg tctgcaggtc agtcacggaa acagaaatca gagaatcggt tagaaacggg
    10501 caccacacgt ttgaggccgt tcaagaagaa acagattgtt ccaccgggtg cggaacctgt
    10561 gaaagccagg tgagatccat cattgcaaac gagctgagtg cgcttcaagc tgcaaaaaaa
    10621 tgaatttcca cacgcaggca tgatctagaa ttcgcgtata tgttctcact gcaatggtct
    10681 gacttgattc gtgctggtgg acctacctta ttcgtcttac tcatttgttt tattgcaact
    10741 tgcatcctga tcattgaacg ttctgcctat tttcgcgcac gcgcaagaga gactgctgcc
    10801 gttctcgaac agttgcgcgc tcaggaagga tctaccgttc ttccgcgaga cagttacaag
    10861 atggtgaatt ctccggttac ttctgtcctg tatgattgtt atccttatgc aggtggatct
    10921 cttttcgagg aagtcaagag tcgtgcgatc gcagaaaacc ttccctcgat tgaaagatac
//
LOCUS       DAOQJL010000056        10975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3058_length_10975_cov_4.653114, whole genome shotgun sequence.
ACCESSION   DAOQJL010000056 DAOQJL010000000
VERSION     DAOQJL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3058_length_10975_cov_4.653114"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLG78_03985"
     CDS             <1..382
                     /locus_tag="PLG78_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HNE22298.1"
                     /translation="HWIGSPESTGAQKRFCMFLRWMTRTTFPDFGLYKTFHPRDLIVP
                     LDVHMARMAQNLNWTTAAGAGWKNAVEITRSLATIEPEDPLRFDFVITRPGILGVCRS
                     ILKPACDHCPVRRPCKVYGQKILA"
     gene            410..1084
                     /locus_tag="PLG78_03990"
     CDS             410..1084
                     /locus_tag="PLG78_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE22299.1"
                     /translation="MRVLCISAHPDDVEIGMGGTVASLTQKNIQVRILDLTNGEPTPM
                     GSPEIRAAESARAAEILKAERRTLDMPNRYLEDTIENRKKLASEIRDFRPEYMFAPYF
                     DDAHPDHIAASHLCDAARFYAKLTKSDIPGEPFFPRRIIYYFPVHIRLRVEPTFLLDI
                     SAHLETKKSAILCYQSQFVLSGKTHLIEHLMTENRYWGFQGGCDAAEPFFQKELPLIR
                     WPEGIA"
     gene            1081..2487
                     /locus_tag="PLG78_03995"
     CDS             1081..2487
                     /locus_tag="PLG78_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22300.1"
                     /translation="MIYFSDTKEQMRDKISRPAPRAPSLCSRPAASFDRSSILRTIPK
                     EKLNGIIILTGHQPTFHHPGILIKNVLAHNLARQSGGIAIHMVHDTDAEEIIFPYPER
                     VSDIVKKKSAKLNDASQILRHESFKPADRSAMLRLTDALRSEVRLVFGPEMIGAIHGG
                     LDLLEKSVREASRPMDVPDRLRLAWEAQNRIDVYTVYSSDLFESSSFQCFFEHVREHG
                     DRFREIYNRLLADYRRAKGIKNPAQPLPDLRPKELPFWVVQHGNRLPFIEGETSDGQI
                     LPRAVTLTLFCRLFFCDVFVHGLGGERYDRITDEILREFFDFAGAPFTAASATLALSS
                     RADLRIFSRSTQEVERDLRAVEFDPTRFLAKTDRVRREKEDLIAEWHKNPASRSRLHR
                     EFVRLNEQARHGLNGVIENLEWEKNLARVANANKTFFGARDLPYFFYDLSEIFKTVND
                     YVFFEKRSPSASLAEARS"
     gene            complement(2430..2768)
                     /locus_tag="PLG78_04000"
     CDS             complement(2430..2768)
                     /locus_tag="PLG78_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22301.1"
                     /translation="MQEKQKQPSKFMRVYKEFEPEEVKRHLLLIDDLYGTCANCGQLG
                     LNFTKDKVCPGCKTTFSFLMTKLKNPGDVIKILNRIKTDSLPFTMIEKDDYDRASARD
                     ALGDLFSKKT"
     gene            complement(2813..3889)
                     /locus_tag="PLG78_04005"
     CDS             complement(2813..3889)
                     /locus_tag="PLG78_04005"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNE22302.1"
                     /translation="MQELRHLSLLYDSIIKPALFRIDPESAHELVAGTVSFVTHSPIG
                     AAVVRSVLGYSSTRLAVTLAGIDFENPLGMAAGFDKTGRLYPFLSCAGFGSVECGTFT
                     RHAQNGNPKPRLFRFPEQQALVNRMGFNNPGAEEAARIIATQKHGAVRGINLGKSKIT
                     DLDGAAEDYLYSLRALVGFADYVAINVSSPNTPDLRKLQEKKRLSSLLRALRKECAPP
                     LILKIAPDLTTEEIEDVLDASSGIIDALILCNTTIDKSSVPGAESLEGGLSGKPLAAR
                     SLALLRQVRAMTGGQLPLISAGGIDSAESAIERIRAGASLIQIYTSYIYGGPDLPRRI
                     LSGIDRYLEKNSMRMPDLIGADAR"
     gene            complement(3868..4488)
                     /gene="lexA"
                     /locus_tag="PLG78_04010"
     CDS             complement(3868..4488)
                     /gene="lexA"
                     /locus_tag="PLG78_04010"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNE22303.1"
                     /translation="MRELTEKQNAILGFIQDYIREQGYPPTIREIARRFKITAKGAYD
                     HMKALEKKGYIKTEKNRSRAMEILKSADGSAPVHPADTVNVPLVGRVAAGLPILAEEN
                     VDEYLSFPRSMLPDAGIFALRVAGESMKDAGIYDGDIAVVKKCDTAENGEIVVALLED
                     EATLKYFYKDKKKVKLVPANSAFRPILTDTVTIVGKLVGIYRSYGI"
     gene            4579..5745
                     /locus_tag="PLG78_04015"
     CDS             4579..5745
                     /locus_tag="PLG78_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE22304.1"
                     /translation="MSVKKTARNSQSCGVPGQPENRPGGPVETLARLLIVAAYGALAY
                     LLWGMLAGILFPVLAALLLAYLLSPLITWMGKKGISRGLAIALVFVGFLLLFFSTVVV
                     LYPVIAREVVTVVEKFPGLIDLIENKTIPWLRDTAGVDVPTTVSGAMENYGESLKQAF
                     PGMLNKAGTWFVAAATSTGSILSGILNVVLIPVFTFYFLADFQIIVSFFENLIPDHRR
                     ERTVAALSRVDAAVGNWLRGQIEVAIVLSLLYGTGLAIVFGIFGLGAKTGFAIGALTG
                     VLNIIPYVGVGTGLVLSLAITLLEWQGIGAVISTASVFVGVQIMDGYFITPRIVGEKV
                     GLGPAAVIISLLVGGGLFGLAGMLLAIPVAAALRTLWPDILEKYKSSRFYTGSP"
     gene            complement(5748..6149)
                     /locus_tag="PLG78_04020"
                     /pseudo
     CDS             complement(5748..6149)
                     /locus_tag="PLG78_04020"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
     gene            complement(6189..6995)
                     /gene="murI"
                     /locus_tag="PLG78_04025"
     CDS             complement(6189..6995)
                     /gene="murI"
                     /locus_tag="PLG78_04025"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNE22305.1"
                     /translation="MADSRPIGVFDSGMGGLTVLSELVRRLPNERFIYLGDTARVPYG
                     SRSPRTVLRYSLEIAAFLGQSDIKMLVVACNTATAHALPELRARMSIPVIGVIDPGVR
                     ALLSRTKSRKVGVIGTRSTIKSGAYETAIRGIDPGMDVTSLACPLFVPIVEEGWMNKD
                     FARLAVREYLTALRDHGVDAVVLGCTHYPLLRRAIESEFPSFTLVDSSVEIADAVTAM
                     IQERDAASAGPGSVRILVTDVTEQVSDLKRLFFPHPVETIEEVVVPELHV"
     gene            complement(6988..8211)
                     /locus_tag="PLG78_04030"
     CDS             complement(6988..8211)
                     /locus_tag="PLG78_04030"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNE22306.1"
                     /translation="MLIVQKFGGTSVGDTDRIKNVANRIKGYYEQGHRLIVVVSAMGH
                     TTDELIDMAMKLNGNPPRREMDMLLSTGEQISMALMAMALEAIEVPAISFTGGQLQIM
                     TDGTHSNARIRGLDTTRLDEAFAQKKVCIVAGFQGVNENQEIMTLGRGGSDTTAVALA
                     VAVKADECEIYTDVDGVYTTDPNKVPKAKKLKQICYEEMLELASMGAGVLHNRSVELA
                     SKFGIVVHVRSSFNHNPGTLVVPEEKIMDMEKVLVRGVSLKSDQARVAALDIPDRPGL
                     AAGLFEKLATASINVDMIVQSSGHDGKNTISFTVPSNDLANARGILEKFLAELGGGRI
                     AVEENIAIVSAVGVGMKSHSGVAATMFQALAKENINIEMISTSEIKISVVVQKDQAKR
                     ALQAAHTAFGLDQNG"
     gene            8337..9269
                     /locus_tag="PLG78_04035"
     CDS             8337..9269
                     /locus_tag="PLG78_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE22307.1"
                     /translation="MKQSSKSHRQKATKPVDTLQRLLGRRATTVRYQSGFLVRGRLHP
                     FLDLAKDQGDAGWSNDIANDLEEHSQHHFIDVYNRRLAVEGLRPALSIKGAAFLDAGC
                     SSGYLLADVQAAFPDASLFGADFFPAGLAHCHRAHPEIPLFRMDLTACPFPDNSFDAL
                     SCLNVLEHIQDDLAAARTLFRILKPAGLAAITVPAAPHLYDLFDEIHFHERRYSLLQT
                     QDLLRAAGFKIRFANYFGSLIYPAFYAVKRRNQNRYGALSFEQKRDLAMSQIQKTSQS
                     RIMNAVCRFEEAIGKRLTYPFGIRACVIAEKPPG"
     gene            complement(9292..10149)
                     /locus_tag="PLG78_04040"
     CDS             complement(9292..10149)
                     /locus_tag="PLG78_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE22308.1"
                     /translation="MITGASGFIGSRACSFFRNAGYLVRAHSRSPIPDQDVVVGDLQT
                     EDAIEGACKDVDGIFHIAGLAHSRGVDEGALERVNVRWTRELANAAKRRSVPFLFISS
                     SKVYGETGHFSEASPLNPQDAYSRAKVRAEDEIQKATDRFVILRPPPVYGVGSKGSLR
                     FLIRAVQKGWPLPVGSIRSLRSYVFVDNLLSAAEMFLRSERSGIWNVTDDEDVSLADL
                     AHRIARASRSSDFSFRFPLFPLRVMGILRPGMYDKVAGEFTLDIGRLKACGYEPSFSV
                     NEGLRIACS"
     gene            complement(10264..>10975)
                     /locus_tag="PLG78_04045"
     CDS             complement(10264..>10975)
                     /locus_tag="PLG78_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE22309.1"
                     /translation="VRSVMNQTHPVEYIVVDGASQDGTVDVVRELLSQSTLNYQIISE
                     RDRGMYDAMRKGIAASHGDVVAILNADDRFESNESVEKVASAFAPDIDVVIGDVIITD
                     QDFGKVLRYYSASAFRPEDLTRGIMPPHAGFYARRHLFEKLGSYTPDYRYAGDFELIL
                     RFLLKARVKFRYLPEILVRMRTGGLSNRSLRHRFNLTKEVLRALKENGYPSSWLLVLS
                     KIPGKLFQYFRRPPGQAR"
BASE COUNT         2638 a         2921 c         2735 g         2681 t
ORIGIN      
        1 acactggatc ggatcgccgg agagcacagg agcgcaaaag agattttgca tgttcctgcg
       61 atggatgaca cgaaccacat tccccgattt tggactgtac aaaacatttc atccgcgaga
      121 tctcattgtg ccactcgatg tgcatatggc cagaatggcc cagaatctaa actggaccac
      181 ggcggccggc gccggctgga agaacgctgt ggaaatcaca cggagtctgg cgaccattga
      241 gccggaagat ccgcttcgtt tcgattttgt gatcactcga cctggaattc tgggcgtttg
      301 cagatccata ctgaagcctg cctgtgacca ctgcccggtc cggcggccat gcaaggtata
      361 cgggcaaaaa atcctcgcct gagtgtagtt ttgcgaattt catgctctgg tgcgcgtcct
      421 ttgcatctca gctcacccgg acgatgtcga aattggcatg ggtggcactg ttgcgtctct
      481 cacccaaaag aacattcagg tgcgcatcct ggatctaacg aacggagagc ccacgcccat
      541 gggtagtccg gaaattcggg cagcggaatc tgcgcgtgca gcagaaattc tcaaagcaga
      601 aagacgcact ctggatatgc ccaaccgcta tctggaagat accatcgaaa acagaaagaa
      661 acttgcttct gaaatacgtg actttcgtcc ggagtatatg tttgcgccgt acttcgatga
      721 cgcgcatccg gatcatatcg ccgcaagcca tctctgcgac gcggcgcgat tctatgcgaa
      781 gctaacaaag tccgacattc ctggagagcc gttttttcca aggcgcatca tctattattt
      841 tccggttcac attcgcctgc gcgtggaacc cacgtttctt ctggatataa gcgcgcacct
      901 ggaaacaaag aaatctgcga ttctctgtta ccagtcgcaa ttcgttctca gcggcaagac
      961 tcatctcata gagcatctga tgacggagaa tcggtactgg ggattccagg gtggatgtga
     1021 tgcggcagag cccttttttc aaaaggagct tccattgatt cgctggccag agggaatcgc
     1081 atgatttact tctccgacac aaaggagcaa atgcgcgata agatctcgcg gcccgctccg
     1141 cgtgcgccca gtctctgtag cagaccggcc gcgagcttcg atcgatcttc tattcttcgc
     1201 accatcccaa aagaaaagct aaacggcatc atcattctca cgggacacca accgacattt
     1261 catcatcctg gaattctaat caaaaacgtg ctggcacaca atctggccag acagagcggc
     1321 ggaatcgcca ttcacatggt gcatgacacg gacgcagaag aaatcatctt tccctacccc
     1381 gaaagagtgt cagacatagt aaagaagaag tcagccaaac tcaatgatgc ttctcaaata
     1441 ctcaggcatg agtcattcaa acccgcggac agatctgcga tgcttcgcct aacagatgct
     1501 ttgcgatcgg aagtgcggct cgtgtttggc ccggagatga tcggagcgat tcacggagga
     1561 ctggatttgc tcgaaaaatc cgtgcgggaa gcaagccggc ccatggatgt gccggatcgt
     1621 ctcaggcttg cctgggaagc gcaaaaccgc atagacgtgt acaccgtata ctcaagcgat
     1681 ctatttgaga gttcttcctt ccagtgcttc tttgaacacg tgcgcgaaca cggagataga
     1741 ttcagagaaa tatacaatcg ccttctggcg gactatcgtc gtgcaaaggg aatcaagaat
     1801 ccagcccagc cactgccaga ccttcgccct aaagaacttc ctttctgggt agtccagcac
     1861 ggaaatcgcc tgccgttcat agaaggggaa acgtcagacg gacagatttt gccgcgcgct
     1921 gtcactctca ctctattctg tcgtctcttc ttctgcgatg tatttgtgca cggacttggt
     1981 ggagaaagat acgatcgcat aacggatgaa atccttcgcg aattttttga ttttgcagga
     2041 gcgcctttca ctgctgcgtc tgctacactt gctctctcct cgcgcgcgga tctccgcatc
     2101 tttagcagat ccacccagga agtggaacgc gatctaagag cagtagaatt cgatcccaca
     2161 aggttcctcg caaaaacaga tcgcgttcgg cgggaaaaag aggatctcat tgcagagtgg
     2221 cacaagaacc cggcttcgcg ctctcgtctg cacagggaat ttgtaaggct caacgaacag
     2281 gcaagacacg gactgaacgg agtgatcgag aatctagaat gggaaaagaa tctggcgcgc
     2341 gttgcaaacg caaacaaaac gttctttgga gcgcgagacc ttccgtattt cttctatgac
     2401 ctgagcgaaa tcttcaagac tgtaaacgat tacgttttct ttgagaaaag atctcccagc
     2461 gcgtcccttg cggaagcacg atcgtagtcg tctttttcaa tcattgtaaa aggcagagaa
     2521 tccgtcttga ttcgattgag tatcttgatc acgtccccgg ggttcttcaa tttagtcatc
     2581 agaaaagaga aagtggtttt gcagccaggg cagactttgt ctttcgtaaa attcaatccc
     2641 agctgtccgc agttcgcgca ggttccgtac agatcgtcta tgagaagcag gtgacgcttc
     2701 acttcctctg gctcgaattc cttgtacacg cgcatgaact ttgagggctg cttttgcttc
     2761 tcttgcatgc tatgtccgtg gcccgcacgt cttttgaacg caagacaaat tttcagcgag
     2821 cgtctgcgcc gatcaggtcc ggcatacgca tgctgttctt ttccaggtag cgatcgattc
     2881 cggatagaat gcgccgcggg agatctggtc cgccgtaaat gtaggaagtg taaatctgga
     2941 tcaggcttgc gccggcccgg attcgttcaa tggcggactc agcagaatca attccgccgg
     3001 cagatataag aggaagctgg ccaccggtca tagctcgcac ctgacgaaga agtgcaagac
     3061 ttcgcgcagc aagcggttta ccagataatc ctccctccag ggattcagca cccggaacag
     3121 aagatttatc aatcgtagtg ttgcacagga tgagcgcatc tatgatgcca ctcgaagcat
     3181 ccaggacatc ctcaatttct tctgtagtga gatctggcgc gatctttaga atcaagggag
     3241 gcgcgcattc ctttctaagc gcgcgaagaa gagaggagag tcttttcttt tcctgcagct
     3301 tgcgaagatc cggagtgttg ggagagctca cattgattgc gacgtagtca gcaaatccaa
     3361 ccagagcgcg aagagaatac aaataatctt ctgccgcacc gtccagatcc gtaatcttgc
     3421 ttttaccaag attgattccg cgcacagctc catgtttttg agtggcgatg atccgggctg
     3481 cttcctccgc tcctggattg ttgaacccca ttcgatttac gagagcctgt tgttccggaa
     3541 aacgaaacag tcttggcttt ggatttccat tctgagcgtg gcgcgtgaat gttccgcatt
     3601 ctacagagcc aaagccagcg caggatagaa atgggtagag gcgacccgtc ttatcaaagc
     3661 cggctgccat tccgagaggg ttttcaaaat cgatgcctgc gagagtgacg gcaagcctgg
     3721 tggatgaata gccaagaacg ctgcgaacga ctgcggcgcc gataggagaa tgtgttacga
     3781 acgaaacagt acctgccacc agctcatgcg cggattcagg atcgatccga aacagagctg
     3841 gtttgatgat ggagtcgtaa agtaaactca gatgccgtag ctcctgtata tgccgaccag
     3901 ttttccaacg atggtcacag tgtctgtcag gattgggcga aaggcagagt ttgccggaac
     3961 cagtttcact ttctttttat ctttatagaa gtatttgaga gttgcttcat cttccagaag
     4021 tgccaccacg atttccccgt tttctgcagt gtcgcatttc ttaacgactg cgatgtctcc
     4081 gtcatagatg ccagcgtcct tcatggattc tccggccacg cgaagagcga aaattcctgc
     4141 atccggaagc atggaacgag gaaagctgag gtattcatct acattctctt ccgcaaggat
     4201 aggcaagcct gcggccacgc ggcccaccag aggcacattg actgtgtctg ccggatgcac
     4261 aggtgcactt ccatccgcgc tctttagaat ctccatggcg cggcttcgat tcttttctgt
     4321 cttgatgtag cccttttttt caagtgcttt catatgatca taggcgcctt tggccgtgat
     4381 cttgaaacgc cttgcgattt cacgaatggt gggcgggtaa ccctgctcgc gaatgtagtc
     4441 ctgaatgaag cccaggatgg cattttgctt ttccgtcaac tctctcatgt ttgctataca
     4501 aacgacacgc aaaaacctgt caagcaaaac cgaaatcctc cgaattgact ccagcaccag
     4561 cgcccccagc cttgcgggat gagcgtcaaa aaaacagcca gaaacagcca gtcctgtggc
     4621 gtccctggcc agccggagaa tcgtccaggt gggcctgtgg agaccctggc tcgacttctg
     4681 atcgtggctg catatggtgc acttgcgtat ctgctgtggg gcatgctggc ggggattctg
     4741 tttccagttc tggccgcttt gcttcttgcg tatttgcttt ctccgctcat cacatggatg
     4801 ggtaagaaag ggatttcgag aggacttgcc atcgcgcttg tttttgtcgg attcttgctt
     4861 cttttctttt ccacggttgt ggttctttat cccgtcatcg caagagaagt ggtcacagtc
     4921 gttgaaaagt ttcctggtct cattgatctt atagaaaata aaaccatccc gtggctcaga
     4981 gatacggccg gagtggatgt gcctactact gtctccgggg cgatggaaaa ttacggcgaa
     5041 agtctaaagc aggcattccc aggaatgctc aacaaagcag gcacctggtt tgtagctgcg
     5101 gccacttcta ccggaagcat tctatctgga atcctgaacg ttgttttgat tccagtcttc
     5161 acgttttact ttctcgctga ttttcaaatc attgtatcct tcttcgagaa tctcattccg
     5221 gatcacaggc gcgaacgaac cgtcgctgcg ctctcgcgcg tagacgcagc tgtaggcaac
     5281 tggcttcgcg gacaaattga agtggctatc gtcttatcgt tgttatacgg aacgggcctt
     5341 gccatagtat ttggaatctt tggcctgggt gcaaagactg gatttgccat cggagcgctt
     5401 accggtgttc tcaacatcat cccttacgtc ggagtcggca ctggccttgt cctttctctt
     5461 gcgatcactc tactcgaatg gcagggaata ggcgctgtta tctctactgc ttctgtattc
     5521 gtgggcgttc agatcatgga tggatacttc atcactccac gcattgtagg agaaaaggtt
     5581 ggtctgggac cagcagcagt cattatatcc ctgctggtag gcggaggatt gttcggtctg
     5641 gctggaatgc ttcttgcgat tcctgtagcg gcggcgcttc gcacactgtg gccggatatc
     5701 cttgagaaat acaaatccag cagattttac accgggtctc cgtagtttta aggagagaaa
     5761 aagcgatgga tgttgaaccc aactgtccaa tcacgcttac gataattgta ggccgcaccg
     5821 cgaaggagag tcgtataggc aaggtctcgt gaatttgtaa caatgagttg gaagatatgt
     5881 ccaccggtat cgatattcac tccgatgctc gctggaatgc ttgcatatcg taccggctca
     5941 tcctgaacca cattgaccac ataagcatag agtaaaccag caggggaaag gtaccttgca
     6001 ttgatgtccg atgctgtaag ctcggttcca tatttagaac gttgatcctc aagtgcatag
     6061 ttggatgcca gatagtcgcg atgcgccgtg agcatggttt ccagcataat gtcgattcgc
     6121 ttcgtgatct taatgcggcc accaaggtca acgcccggaa cctggcttcg ccagggtgag
     6181 tcccaggttc agacgtgaag ctccggtaca acaacctcct cgattgtttc taccggatga
     6241 ggaaaaaata aacgtttcag atcagagacc tgctctgtaa cgtctgtaac tagaattcga
     6301 acactgcctg gccccgccga agccgcgtct cgctcctgga tcattgcagt caccgcgtct
     6361 gcgatttcaa cagacgaatc tactagagtg aaagaaggaa attcgctttc gatagcgcgt
     6421 ctgaggagcg gataatgagt gcaaccaaga acgaccgcat ctacaccgtg atcccggagt
     6481 gcagtcagat attcgcgcac cgccagtcgt gcgaaatctt tattcatcca gccttcttct
     6541 acgatgggaa caaagagagg acaggcaagg ctcgttacgt ccattccagg atcgattccg
     6601 cgaatcgctg tttcgtaggc gccagatttg atggtagatc tggtcccaat gactcctact
     6661 tttctgctct tcgtgcgaga caagagagcg cgcacccctg gatcaatcac gccgatgact
     6721 ggaatggaca ttctggcgcg aagctctgga agagcatggg ccgttgcagt gttgcatgcg
     6781 accacgagca ttttgatgtc gctctgcccc agaaaggctg caatttctag agaatagcgt
     6841 aggaccgtgc gaggcgatct ggatccgtaa ggaacacgcg ccgtgtctcc gaggtaaatg
     6901 aatcgttcgt tcggaagccg gcgcaccaat tcggacagaa cggtcagccc gcccatgccc
     6961 gaatcgaaca cgccaatggg gcgtgaatca gccattctga tccaggccaa atgcagtgtg
     7021 tgccgcctgc agtgctcttt tggcctgatc tttttgcaca acgactgaga tcttaatctc
     7081 agatgtgctg atcatttcaa tgttaatatt ttctttcgcc agtgcctgga acatggtggc
     7141 ggcaacacca gaatgagatt tcattccaac accaaccgcg gagacgatgg cgatgttctc
     7201 ctctaccgcg attctacccc cacccagttc cgcgaggaac ttctctagaa tgccacgtgc
     7261 attcgcaaga tcgttagatg gaactgtgaa cgaaatagtg ttcttcccgt catgtccact
     7321 gctttgaacg atcatatcga cgttgatgct tgcagtggca agtttctcga aaagtccagc
     7381 agccagtccc ggtctgtccg gtatatcgag tgctgccaca cgtgcctgat ccgatttaag
     7441 gctcacgccg cgtacgagta ctttttccat atccatgatt ttttcctccg gcacgaccag
     7501 tgtgcccggg ttgtgattaa aacttgatcg cacatgcact acgatgccaa acttggacgc
     7561 aagctccacg cttcgattgt gaagaacgcc cgctcccatg ctcgcgagtt ccagcatttc
     7621 ttcgtagcag atttgtttca attttttagc ttttggaact ttgtttggat ccgtagtgta
     7681 gacgccgtcc acgtccgtgt agatttcgca ttcatccgct ttcactgcaa ctgcgagagc
     7741 cactgcagtc gtatcgcttc cgccacgacc cagggtcatg atttcctgat tttcattcac
     7801 gccctgaaaa ccggctacga tacatacttt cttctgcgcg aaggcttcat ccagtctggt
     7861 cgtgtccagt ccgcgaatgc gagcgtttga gtgagtccca tctgtcatga tctgcagctg
     7921 gccacccgtg aatgaaatcg cggggacttc aattgcctca agagccatgg ccatgagagc
     7981 catagagatt tgttctcctg tggacagaag catgtccatt tcgcgccggg gaggattccc
     8041 gttgagcttc atggccatgt cgatgagttc gtccgtcgtg tggcccatgg cggacacaac
     8101 cacaatgagc ctgtggccct gctcgtagta gcccttgatg cgattggcaa cattcttgat
     8161 gcggtccgta tcgcctacag aagtgcctcc gaatttctga acaatgagca taatgctaga
     8221 tctttccagg cgtccgtgcg gacaagattt ttagaaacag tagcccaccc atccgcccca
     8281 aaaatgattg caaagatcga ccaaaaaccg gtcctgcaac ctccatccaa aacagaatga
     8341 agcaatcgag caaatctcat cgccagaagg ccacgaaacc tgtagacaca ttgcagaggc
     8401 tcctcggtcg ccgggccacc acggtgcgat atcagtctgg ctttcttgtt cggggtcgcc
     8461 ttcatccgtt cctggatctg gcgaaagacc agggcgacgc cggctggtcc aacgacatag
     8521 cgaatgatct ggaagagcat tcacagcatc acttcataga cgtgtacaac agacgcctcg
     8581 cagtggaagg gttgcgcccg gctctatcta taaaaggcgc tgcgttcctg gatgctggct
     8641 gttcatccgg ttaccttctg gcagacgtcc aggcggcttt cccggatgcc tctttgttcg
     8701 gtgcggactt ctttcctgcc gggctcgcac actgccatcg agcgcacccg gaaattcctt
     8761 tatttcgcat ggatctcact gcctgcccgt tcccggacaa ctctttcgat gcattgagtt
     8821 gcctgaacgt ccttgagcac attcaggatg atctggccgc tgctcgaact ctgtttcgca
     8881 tcctaaagcc ggccggcctc gcagccatca ctgtcccggc tgctcctcac ctgtatgatc
     8941 ttttcgatga gattcatttt catgaaagac gttattcgct cttgcaaacg caagacttgc
     9001 tgcgtgctgc tggattcaag attcgctttg ccaactactt tggatctctg atctaccctg
     9061 cgttctatgc agtcaaacga agaaaccaaa acaggtacgg agcactgagc tttgagcaaa
     9121 aacgagacct ggcaatgagc cagattcaga aaacgtctca atccagaatc atgaatgcag
     9181 tctgtcgctt cgaagaagca atcggaaagc gcctgacgta tccattcgga atccgcgcct
     9241 gtgtgatcgc agaaaaaccg ccaggttgaa cctgagatcc ttcctgcgaa gtcaactgca
     9301 ggctatgcgt aaaccttcat tcacggaaaa agatggctca tagccgcatg ccttcaagcg
     9361 accaatgtca agagtgaact caccggccac cttgtcgtac atgcctgggc gcagaattcc
     9421 catcactctc agggggaaaa gtggaaatcg aaaactgaaa tcagaactcc gggaggcacg
     9481 tgcaatgcga tgagccagat ccgcaagaga tacatcctcg tcgtctgtta cgttccagat
     9541 tccggatcgc tcactccgca aaaacatctc cgcggcagaa agcaggttat ctacaaacac
     9601 atagcttcgc agactgcgaa tgcttcccac aggcagcggc catcctttct gtactgcgcg
     9661 aatcaagaaa cggagactgc ctttgctgcc aacgccataa accggaggcg gacgtagaat
     9721 cacaaagcga tctgtggcct tttgaatttc atcttccgcc ctcaccttcg cccgagagta
     9781 tgcatcctgc ggattcaatg gcgacgcttc cgaaaaatgt ccggtctccc catacacttt
     9841 ggaactgctg atgaaaagga atggaacaga tcgtcttttc gcagcattcg caagctcgcg
     9901 cgtccagcga acgttgactc tctcgagggc tccttcatct actccgcgcg aatgtgcaag
     9961 accggcaatg tgaaaaatcc catccacgtc cttgcaggcg ccttctattg catcctcggt
    10021 ctgaagatct ccgactacaa cgtcttgatc aggaatgggt gagcgcgaat gtgcgcgcac
    10081 aagatagccc gcattccgaa aaaacgagca agcgcgcgat cctataaaac cactggcccc
    10141 cgtgatcaat atggtcttca aaaccgagcg gcctcttgcc tgtacatagc ttcgccccgg
    10201 gagccacaaa atcgcctatc gcgcactaag aaccatatcg actgaattgc gcgcatttgt
    10261 tcctcaccgg gcctgcccgg gtggacgacg aaaatactgg aagagttttc ctggaatctt
    10321 ggatagaaca agaagccagc tggaagggta accgttctct ttcaaggccc tcagcacttc
    10381 ttttgtcaga ttaaaacgat gacgcagact gcgattgctg agtccgccgg ttcgcatgcg
    10441 aaccagaatt tccggcagat agcgaaactt gaccctggcc ttcaaaagaa accgcagaat
    10501 gagttcaaag tctcccgcgt aacgatagtc aggcgtgtac gatccgagtt tttcaaagag
    10561 atggcggcgt gcataaaaac cggcatgcgg tggcatgatg ccacgcgtga gatcctcggg
    10621 gcgaaaagca gatgcggaat agtagcgaag aactttccca aaatcctgat ccgttatgat
    10681 cacgtctccg attaccacat caatatccgg tgcaaatgca ctcgctactt tttccacgct
    10741 ctcattggat tcaaatcgat catctgcatt caggatggcc accacatctc cgtgagaggc
    10801 agcgattcct tttcgcatcg cgtcatacat acctctgtcg cgttcagaga tgatctgata
    10861 gtttagagtg ctctgtgaaa gaagctctcg aacaacatcc accgttccat cctgcgatgc
    10921 gccatccact acgatgtact ccacaggatg agtctgattc atcacagatc gcacc
//
LOCUS       DAOQJL010000057        10919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3085_length_10919_cov_4.146355, whole genome shotgun sequence.
ACCESSION   DAOQJL010000057 DAOQJL010000000
VERSION     DAOQJL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_10919_cov_4.146355"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..759
                     /locus_tag="PLG78_04050"
     CDS             382..759
                     /locus_tag="PLG78_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNE22310.1"
                     /translation="MASDKSFVQFALDQLKPVSVTCKPMFGEYALYKDNKVVALICDS
                     QLFVKQTEAGKIFAGSIPEAPAYPGAKPSLLATEGLEDPEWICELIRITAKNLPAARP
                     RKKTGAKKKESRANVAAKKKRSK"
     gene            complement(796..1875)
                     /locus_tag="PLG78_04055"
     CDS             complement(796..1875)
                     /locus_tag="PLG78_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNE22311.1"
                     /translation="MSNKLFSPFSARGLDLANRMVMAPMTRNRADGNVPNDLMAEYYG
                     QRAGAGLIVTEGTSPSPNGLGYPRIPGSFNQAQADGWKKVTSAVHAKGGKIFQQMMHT
                     GRIGHQANLPAGAKIVAPSAVAAAGQIYTDSQGMQAHPVPHALSTEEVKSTVQEYVTA
                     AKFAMAAGFDGVELHAANGYLIEQFIHPHSNRRTDEYGGSIENRLRFLVEVASAVAAA
                     IGKDKVGVRLSPYGVFNDLPPYSEVEETYTLAAKKLQEVGILYIHIVDHSSMGAPAVS
                     DSVKASIRKEFQGIVILSGGYDVARANADIDAGKGDLIAFGKQFLSNPDLVVRLEKGK
                     ALNAFDMGTFYTPGPKGYIDYPVAG"
     gene            2003..2272
                     /locus_tag="PLG78_04060"
     CDS             2003..2272
                     /locus_tag="PLG78_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22312.1"
                     /translation="MNGEMEKTEKGSARVDAIKEQAAQLAGKAKEGFARLNERTQVYQ
                     EGAREFLDSVGLYIKENPQRSAIIAVLAGLGLGLLIGRATKRGRD"
     gene            2276..2653
                     /locus_tag="PLG78_04065"
     CDS             2276..2653
                     /locus_tag="PLG78_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22313.1"
                     /translation="MSGKYPALARLRKGQKSAVEEQSESPSLFSSLFTRIHEQWKIRS
                     ARFLAGILLFVSSILLFVISIVFLLGGLYATIKLFVGQIAALFVMGLISLLSGTILLR
                     RSLRNLDESVDFSKKELDLKITD"
     gene            2655..3359
                     /locus_tag="PLG78_04070"
     CDS             2655..3359
                     /locus_tag="PLG78_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE22314.1"
                     /translation="MQYYRLLGLNQGAGPDEIKNAFRSRIKECHPDRVPGDGEKARRI
                     IEAYRGLLKGETGPEPVAAPVRPSPPPRPQGPTIRVHAKSRSHAAGVEAGRRIFRSVF
                     RGHDLAGSVENFWDAVERHVLEEGEETEVYGKHVHVREVPWEQRLGGNGITNPKAREI
                     FERAEVALREVVAKYDAQKQRSRRQWSKDYIFNLAQVQVLFRDAMNRHPSLTGRALSR
                     LQQIHELIGEIRKMMV"
     gene            3363..3560
                     /locus_tag="PLG78_04075"
     CDS             3363..3560
                     /locus_tag="PLG78_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HNE22315.1"
                     /translation="MNTTIKVEGMTCQHCVKAVEKAVSEKGGTARVDLKSGMVELQFE
                     ESKSSLADIKAAIEETGYSVP"
     gene            3610..4125
                     /gene="msrA"
                     /locus_tag="PLG78_04080"
     CDS             3610..4125
                     /gene="msrA"
                     /locus_tag="PLG78_04080"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE22316.1"
                     /translation="MSNLQLATVGGGCFWCLEAVFQRVNGVSKVVSGYAGGRTENPNY
                     REVCGGNTGHAEVIQIEFDPATISYEKLLDVFWKCHDPTTLNRQGADTGTQYRSIILT
                     HDENQKKLAIASRTAAEKNFDSPIVTEIVPLTKFYAAENYHQDYYNQNGHAPYCQVVI
                     APKLKKMGMAP"
     gene            4125..5213
                     /locus_tag="PLG78_04085"
     CDS             4125..5213
                     /locus_tag="PLG78_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNE22317.1"
                     /translation="MSRIAFAHTALPKNVYNQDELIGAFRELWAEKFFNTDRLEQFHR
                     NVMVGQRHLALPIEQYRKLSGFGATNSAYIDAAVELSERLLRELFEKTGFDPRTLNAL
                     ISTSVTGIMVPSIDARLMNRLPLPASLKRMPLFGLGCMGGAAGIARAADYLRGHPKDS
                     VVFLSVELCSLTLQREDLSVANIVSSGLFGDGAAAVLMLGEEHPMAGRITPGMPEKAF
                     RSPTILSNRSIFFPNTERVMGWDIVDSGFQVVLDSSVPEFAEKNLRPGVEAFLKEQGL
                     SLSDIEVWIAHPGGPKVITGMEKGLSLREGTLKLSNESLLKYGNMSSASVLFILKETL
                     DQNPPEKKLGLLLAMGPAFAAEAVLLRW"
     gene            5216..5725
                     /locus_tag="PLG78_04090"
     CDS             5216..5725
                     /locus_tag="PLG78_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE22318.1"
                     /translation="MDSRVLYSAFLVILAIQRLFELRLSKRNATRAFLEGAKEAAPHQ
                     VPWMTAMHSLWFVSCAAEVWMGASFHPGIFILSLAVTVIGQTLRYAAIRTLGPRWNVR
                     IIHFPGKPPVQTGIYRLVRHPNYVGVILEIAAVPMLHGAWITSIAFSIINGIFLYFRI
                     RAEENAVYG"
     gene            5718..5975
                     /locus_tag="PLG78_04095"
     CDS             5718..5975
                     /locus_tag="PLG78_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE22319.1"
                     /translation="MDETTQDIFLLVKRILREKCKVEGQVGIDARLAEDLRLDSVGML
                     TLALEIENHYRMNLGDEPDSPPRTVQEIVTLIKARLSERKS"
     gene            5972..7621
                     /locus_tag="PLG78_04100"
     CDS             5972..7621
                     /locus_tag="PLG78_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE22320.1"
                     /translation="MNTLIDALSEAASTTRGIQFFDRSGSITRFNYSELYADAQKIAR
                     VMAQHGLAPGQSAGLVFSTSYEFVRQLFGVLLAGGVPFCLAPPRLGRAADYSEYTARM
                     INSAGARFVIHEDSIGGSLAKVAARTSSPFIPVSQIVADIASASPETKSVTRDRPALI
                     QFSSGTTVDPKPVVLTHGNLVSNTKSILERFPGDINDHSGLSWLPMHHDMGLIGGLFS
                     AVMGKGDITFLRPEDFVMRPALWLRALSETKATTCPCPNFALKLCTDRIETEDVKDLD
                     LSHWQIALVGAETVHLSTLRDFAEKFAITKFRYAAFTPVYGLAEATLAVTFSDLSSPP
                     HAERFDSALLSQGKAVETSETGGSLEIASVGKPLSGFELQIRDDSGAEAQEGQVGRIF
                     LRGESIMQGYLGQPPHEGWLDTGDLGFIHKGDLYIYGRAKDVIIINGRNHDPAYIEFA
                     TEGVPGIRHERTAAFAVSSDTRATEGFILLVEKEKGDLRNQEEVVTLVRDSVTEKTGL
                     IPDSVHIIEIGQMPRTTSGKVRRAEARRQFLSGKIAALASS"
     gene            7657..8838
                     /locus_tag="PLG78_04105"
     CDS             7657..8838
                     /locus_tag="PLG78_04105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE22321.1"
                     /translation="MIALREFYDAVIVGGGPAGLATAIVLGKENLSVLLIDRRNLPAD
                     KPCGEGLLPPGLEILHDLGVNIPEHMCHPIRGIRYISGNIQAQAEFKEGSGRGIRRTV
                     LSAALLARANEFENVQILSPARAEFPSGLDHFDGTVRISQNNSSAICRTNLIIGADGL
                     HSEIRRLAKLERPGRAKTRFGLRKHFQSNHTLDDFVTVFVAKGSEAYLTPVGQDSFGV
                     AILFSRNEDTRSGKEEAFEALRNRFPGLAPLTQTSAGEQGGAGPLEQRTTGVARDGVI
                     LVGDAAGYLDAATGEGISLALATAQSTARLAQMLRKSNKPLNRLQLSGYEKDHHRITW
                     NYFVMTKMLLFMSDHPTLARPFIRIMAVRPRSFQALLSFNMGEPLFPVLRRFIAGLFR
                     K"
     gene            complement(8846..10126)
                     /locus_tag="PLG78_04110"
     CDS             complement(8846..10126)
                     /locus_tag="PLG78_04110"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22322.1"
                     /translation="MSETLDRLLETRERRGLRFILTFRSVFVFFGLILAFFTPATRWE
                     MFVTIGLFSSFAICTLSGFYLVRRPSLMSVVGWAGVAIDLVMIAVLPFVWLNSVGGGA
                     VVSRAYLLKLDTLTTMSYSIIVMNSVASRPAYPAATTIGAVAYHLFLFYYAGLDSRVV
                     FEENYVAHMLGDRVHPGLLTTNLLVMAGVGACLTFFTHVSRQTLFEAIHREKAALQIS
                     RYFSPSVYNHIAQAEDSFFTSSGRLQEVTVLFSDLRGFTAMSEKMPAADVIRFLREYH
                     ETMVQIIFKHNGTLDKFIGDAIMATFGTPDTAPDDTNRAVAAALEMRGALTQMNANRE
                     SKGLLPLRQGIGIHTGQVIAGNIGTAERLEYTVIGDTVNVASRVESATKEAKTDILVT
                     QSVADRASALAQFEDAGFFDLKGKAEKIRLFRLL"
     gene            complement(10123..>10919)
                     /locus_tag="PLG78_04115"
     CDS             complement(10123..>10919)
                     /locus_tag="PLG78_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22323.1"
                     /translation="KIELFPEGTYPSIEPGKKPVAHVQLVKDASAPDSLYSMVAERHT
                     NRAEYEGPMISPAEIEKVEADAGAGYTKRVAIYGEDMKPYLPLMNSAMELETMTYRLS
                     DETRGWFRFSDREIQEKRDGLGLPDQGVTGFTRWMIETFFLSREPEKFHDPANNKLFL
                     ERYKKKIDSARALYYWSTSSNTIVDWVKTGRDYARFQLALMKAGLRMHPLSQILQEFP
                     EMDDLRAKFEKISGVRAPGKIQMIVRIGRSDYKYFTPRRSAKDMIV"
BASE COUNT         2867 a         2804 c         2702 g         2546 t
ORIGIN      
        1 ggttcatccc ttcaaaagaa atccgagcac attccacaga ttcatccggt ccaatggcgc
       61 atgacgttat tgcagaattg gttggcgcgc gaagatagta tccggagtta atgctcgagc
      121 attgagtaat caatgacacc aaaaaaatgc cgcaaacaag ctgctgaaaa tggcaggtac
      181 tttgtaaatt gtaaaccgtg cccatcgctc tcgaggattt ttcgtgaacg cggtccgtgc
      241 aacagaaaaa agaatcgcag agacaagcct ctgaatcctt caccaacgaa tccatgagtg
      301 ccgcataacg gtaatgggaa aaagcagtca ttttgatctt gcagcgcgag gcccctttgc
      361 gaaaaacagc ctgggcaacc tatggccagt gataaatcct tcgttcaatt cgctctagat
      421 caactaaaac ctgtctcagt cacctgcaag cccatgttcg gagaatatgc actctacaaa
      481 gacaacaaag tcgtcgctct catatgcgac agccagctct ttgtgaagca gacggaagcg
      541 gggaaaatct ttgcgggatc gatccccgaa gcaccggcct accctggcgc gaagccgagt
      601 ctactggcaa ccgagggtct tgaagatcca gaatggatct gtgagctgat ccgcatcaca
      661 gcaaaaaatc taccggctgc ccgcccgcgg aagaaaaccg gtgcgaagaa aaaagaatcc
      721 cgggccaatg tcgcagctaa aaagaagcgc tccaaatagc ccggcggtga cggccgtaac
      781 cggccgcctt ccgattcaac ctgctactgg atagtcaatg taaccctttg gaccaggtgt
      841 gtaaaacgta cccatgtcga aagcattgag cgcttttcct ttttcgaggc gaaccaccag
      901 atctggattg gaaagaaatt gttttccgaa tgcgatcagg tcccccttgc ccgcatcgat
      961 gtccgcgttt gcgcgcgcga catcataccc gccactcaaa atgacgattc cctggaactc
     1021 ctttcgaata gaagccttga cgctatcaga tacagcaggc gcacccatgg aggaatgatc
     1081 cacgatgtga atataaagaa tcccaacttc ctggagtttc tttgcagcca gcgtgtacgt
     1141 ttcttctact tctgagtagg gaggaagatc gttgaacact ccgtaaggtg aaaggcgaac
     1201 tccaactttg tcttttccaa tggctgcggc cacagcagag gcaacttcca ccagaaaacg
     1261 aagtctgttc tcaatcgatc caccgtattc gtcagttctg cgattggaat gcggatgaat
     1321 gaattgttcg atcaaatatc cattcgccgc atggagctct acgccgtcga agccggctgc
     1381 catggcgaac ttagctgcag tcacgtattc ctgaactgtg ctcttcactt cttcggtgct
     1441 caaggcgtgg ggaactggat gtgcctgcat tccctgggaa tcagtgtaga tttgaccggc
     1501 tgcagcgact gcagaaggtg cgacgatttt agcgcctgct ggcaaatttg cctggtgccc
     1561 gattctaccg gtgtgcatca tttgctggaa gattttgccg ccttttgcgt gaactgccga
     1621 ggtcactttc ttccaaccat ctgcctgcgc ctggttaaaa gatcctggaa ttcttggata
     1681 accaagtcca ttgggagaag gagaagttcc ttctgttaca atcaggcctg cgcctgctct
     1741 ctggccgtag tattctgcca tcagatcatt tggaacgttt ccatccgcac ggtttcgagt
     1801 catcggcgcc ataaccatgc gattggccag gtcgaggcca cgcgcggaaa aaggagaaaa
     1861 aagtttgttg ctcatattgc ttcaagttaa aagcaattga acggaacggc aattctatcg
     1921 tctaaaataa atgaaaaggc tcactggtga atcttcgctt tacatctaaa aagataccac
     1981 cagtttgcac cgggagaaaa acatgaacgg cgaaatggaa aaaacggaaa agggatccgc
     2041 tcgagtggac gccatcaaag agcaggcagc gcaacttgct ggaaaagcaa aagaaggctt
     2101 cgcccgtctc aatgaacgca ctcaagtcta ccaggaagga gctcgcgaat ttctcgattc
     2161 agtaggactc tacatcaagg aaaacccgca gagatccgca atcatcgctg tgctggcagg
     2221 acttgggctg ggactcctga taggtcgtgc gaccaaacgc ggccgcgact gatccatgtc
     2281 tggcaaatat ccggccctcg cgcgcctgcg caagggccag aagagcgcag tggaagaaca
     2341 gtcagagagt ccttcgctct tctcgtctct cttcacaaga atccatgaac agtggaagat
     2401 tcgctccgcg cgatttctcg caggcatact gctttttgtg tcctcgatcc tcctattcgt
     2461 aatctcgatc gtatttttgc tcggcggcct ttacgcgacc atcaagctct ttgtcggaca
     2521 aattgcagcg ctttttgtca tgggattgat ttctctgctt tcaggaacga ttctacttcg
     2581 tcgttcgctt cggaatttgg acgaatccgt cgactttagt aagaaggaat tggacctcaa
     2641 gattacagac tgagatgcaa tactatagac tactagggct caatcagggc gccggaccgg
     2701 atgaaatcaa gaatgcattc cgttccagga tcaaagaatg ccatcctgac cgagtacctg
     2761 gagacggtga aaaagcaaga cgcatcattg aagcatacag aggtcttctg aagggagaaa
     2821 caggaccaga gcctgtcgcg gctccagtga gaccttctcc tcctccgcga ccccagggcc
     2881 caaccattcg cgttcacgca aaaagccgca gccacgcagc aggcgtagaa gcaggaagaa
     2941 gaatcttccg atccgtattt cgcggacacg acctggcagg ttcggtagaa aatttctggg
     3001 atgcagtcga acgccacgta ctcgaagaag gcgaagagac agaagtatac ggaaagcacg
     3061 ttcacgtacg cgaagtcccg tgggaacaaa gactcggcgg aaacggaatt acaaatccaa
     3121 aagcacgcga aatttttgaa cgagcagaag tagctctacg cgaagtcgtt gcgaaatatg
     3181 atgcacagaa gcaaagaagt cgccgccagt ggtccaaaga ctatatattc aatcttgcgc
     3241 aggtgcaggt tttgtttaga gatgcgatga atcgccatcc ttcgctcacc ggaagggctc
     3301 tcagcagact gcagcaaatc cacgaactga tcggcgaaat tcgaaagatg atggtttgaa
     3361 caatgaatac tacaatcaaa gtcgaaggca tgacctgcca gcattgcgtg aaagcagttg
     3421 aaaaagcggt ctctgaaaag ggcggcactg ccagagtaga tttgaaaagt ggaatggtag
     3481 aacttcaatt cgaagaatcc aaaagcagtc tggcggatat caaagctgcc attgaagaaa
     3541 ccggttattc tgttccctga tcctggattc ggagtgacga gtccagcccg ccctgatttc
     3601 ttataattca tgagcaatct acagctggcc acagtcggag gcggatgctt ctggtgtctg
     3661 gaggcggtgt tccaacgtgt aaacggcgtt agcaaggttg tgtctggata cgctggaggc
     3721 agaacggaga atccaaatta ccgcgaagtg tgcggcggaa atacaggcca tgcggaagtc
     3781 attcaaatcg aattcgatcc agccacaatc tcctacgaga agctgctcga tgtattctgg
     3841 aaatgtcatg atcctaccac tctcaatcga cagggagcag acacaggaac ccaataccgc
     3901 tccatcattc tcactcacga cgaaaatcaa aagaaactag ccatcgcgtc acgaactgca
     3961 gcagaaaaaa atttcgattc acccattgtg actgagattg ttccgctcac gaagttctac
     4021 gccgccgaga attatcacca ggactattac aaccagaacg gacatgcgcc ttactgccag
     4081 gtagtgatcg cacccaagct gaagaaaatg ggcatggcac cctgatgtct cgaatcgctt
     4141 tcgcgcacac tgctctaccc aaaaacgttt acaatcagga tgaactgatc ggtgcctttc
     4201 gcgaactatg ggcggagaag ttttttaaca cagacagact cgaacaattt catcgcaatg
     4261 taatggtggg acagcgtcat ctcgcattgc ctattgagca gtatagaaaa ctgagtggct
     4321 ttggtgctac aaacagtgca tacatagacg cagctgttga actctcagaa agacttctac
     4381 gagaactgtt tgaaaagaca ggctttgacc ccagaacttt aaacgcactc atttccacca
     4441 gtgttaccgg catcatggtt ccgtctatcg acgcaaggct gatgaatcgt ctgcctcttc
     4501 ctgcttcact gaaaaggatg ccgttattcg gccttggttg catgggtgga gcggccggca
     4561 tcgcacgcgc tgcagactac cttcgcggac atccaaaaga ttccgttgtg ttcttgtccg
     4621 tagaactctg ttcgctcact ctacagagag aagacctctc tgttgcgaat attgtttcga
     4681 gcggcctttt tggagacggc gctgccgccg tgttgatgct cggagaagaa catccgatgg
     4741 cggggagaat cactcctgga atgcctgaaa aagcatttcg atctccaacg attctctcta
     4801 atagaagcat ctttttccca aacacggaac gagtgatggg ctgggatatt gtggacagcg
     4861 gattccaggt cgttttggat tcgtccgttc ctgaattcgc ggaaaagaat ttaaggcccg
     4921 gagtggaagc gttcctcaag gaacaggggc tttctctttc agacatagaa gtgtggatcg
     4981 ctcatccggg tggacccaaa gtaatcaccg gtatggaaaa gggactctca ctgcgcgaag
     5041 gaactctcaa gctttccaat gaaagtcttc tcaaatatgg caacatgtct tccgcctctg
     5101 tgcttttcat tctcaaagag acactcgatc agaatcctcc ggaaaagaaa ctggggcttc
     5161 tcctggctat gggtccggcc ttcgcagcgg aagcagtcct gttgcgatgg tgagcatgga
     5221 ttccagagta ctctatagcg ccttccttgt gatcctcgcc atacagcgcc tgttcgaact
     5281 cagattgagt aagagaaatg caaccagggc ttttctggaa ggagcaaaag aagcagctcc
     5341 ccatcaggtc ccgtggatga cggccatgca ttcgctctgg tttgtttcct gtgctgcgga
     5401 agtctggatg ggtgcatcct tccacccggg tattttcatt ctgtcactgg ctgtcactgt
     5461 gattggacaa actctacgtt atgcggcgat tcgcacgctc gggcctagat ggaatgtgcg
     5521 catcattcat tttcctggaa agcctccagt ccagacagga atttatcgcc tggtgcgtca
     5581 cccgaactat gtgggagtga tccttgaaat tgcggctgtt cccatgttgc acggcgcgtg
     5641 gatcacatcc attgcctttt ctatcataaa tggaattttc ttgtacttca gaatcagggc
     5701 agaggaaaat gcagtatatg gatgaaacga ctcaggacat cttcctgctc gtaaagcgga
     5761 tcctgcgcga aaaatgcaaa gtggaaggcc aggttggaat agacgcgagg cttgcggaag
     5821 atctgagact cgattccgtt ggaatgctca ctcttgcgct cgaaattgag aaccactatc
     5881 gtatgaatct tggcgatgag ccagatagcc cgccgcgaac cgtccaggaa attgtaactc
     5941 taatcaaagc aagattgagc gagagaaaat cttgaacact ctcattgatg ccctgtctga
     6001 agccgcttcc acaacgcgtg gaattcaatt ctttgatcga tccggaagca tcacgcgatt
     6061 caattattcc gagctctatg cagacgcgca aaagatcgca cgcgtgatgg ctcaacatgg
     6121 acttgctcca ggtcaaagcg cagggcttgt attttccact tcttacgaat tcgttagaca
     6181 actgtttggt gttctgctgg cgggcggcgt gcctttctgc ctagcacctc cgagactggg
     6241 gagagcagca gactactctg agtacactgc tagaatgatc aactccgcag gtgctcgttt
     6301 tgtcattcat gaagattcaa ttggtggatc actggcaaaa gtagcggccc gcacttcttc
     6361 tccgtttatt ccggtttcgc agatcgtcgc ggacattgct tctgcgtctc ctgaaaccaa
     6421 gagcgtcacg cgggatcgac ccgcactcat tcaattttca agcggcacga ctgtagatcc
     6481 aaagcccgtt gttttgactc acggaaacct ggtttcaaat acgaaatcaa ttctggaaag
     6541 atttccagga gatataaacg atcacagcgg cctgtcctgg ttgcccatgc atcacgatat
     6601 gggcctcatt ggcggattat tttcagcagt catgggaaaa ggagacatca cgtttcttcg
     6661 ccctgaagat tttgtaatga gacctgccct ctggttgcgc gcactcagcg aaacaaaagc
     6721 aaccacatgc ccctgtccca atttcgcgct caagctatgc acggatagaa ttgaaacaga
     6781 agatgtaaaa gacctggatc tttcccactg gcaaattgct ctcgtaggag cagaaacagt
     6841 tcacctctct actttgcgag acttcgctga aaaattcgca atcacaaagt tccgttatgc
     6901 ggcattcaca ccggtctatg gtcttgcgga agcaactctt gcagtcacat tctccgatct
     6961 atccagtcct ccgcatgcag agcgatttga ttctgctctt ctttcgcagg gcaaagcagt
     7021 cgaaacaagc gagactggcg gaagccttga gattgcatct gtgggaaaac cactgagcgg
     7081 atttgagctc caaatccgcg acgattcagg agcagaggcg caagaaggcc aggtaggcag
     7141 aatctttttg cgcggagaat ccatcatgca ggggtacctg ggacaaccgc ctcatgaagg
     7201 gtggctggac acgggggatc ttggttttat tcacaaggga gacctctaca tttatggccg
     7261 cgcaaaggat gtaatcatca tcaacggaag aaatcatgat cctgcttata tcgaattcgc
     7321 gacagaagga gttccaggaa ttcgccatga gcgaaccgct gcctttgcag tttcatccga
     7381 cactcgcgca acggagggat ttatcctgct tgtggaaaag gaaaaaggag atctgcgaaa
     7441 tcaagaagag gtcgtgactc ttgtgcgcga ttctgttaca gagaagaccg ggctcatccc
     7501 tgattcagta cacatcattg aaatagggca aatgcctaga accaccagcg gcaaggtaag
     7561 gcgcgcagaa gccagacggc agttcttatc cggaaagatc gcggctctcg catcgagctg
     7621 attttgcccg catgaagcag tgatgcgtag aaaactatga tcgcccttcg cgaattctac
     7681 gacgcggtca ttgtaggcgg tggacctgcc ggacttgcaa cggccatcgt cctcgggaaa
     7741 gaaaaccttt ccgtgctcct catagacagg cggaaccttc cggcggacaa accatgcgga
     7801 gaaggccttc ttccgcccgg gcttgaaatt ctacatgatc ttggagtgaa cattccggaa
     7861 cacatgtgtc acccgatccg cggaatccgc tacatctccg gaaacatcca ggcacaggca
     7921 gaattcaaag aagggtcagg aaggggaatt cgcagaacgg tgctctccgc agcgttgctc
     7981 gcacgcgcga acgaatttga aaacgtccag attctctcac ccgctcgcgc agaatttccg
     8041 tcaggcctgg accatttcga cggcacagtg cgcatttcgc agaacaactc aagcgcaatc
     8101 tgtcgcacaa atctgatcat aggagcagac ggccttcatt cggagatccg aagactggca
     8161 aaactcgagc gccccggaag agcgaagact cgcttcgggc tgcgaaagca tttccagtcc
     8221 aatcatacgt tagacgactt tgtgactgta tttgtagcca aaggttccga ggcctatcta
     8281 acacctgtcg gccaggattc attcggtgtg gccatcctgt tttccagaaa tgaagatacg
     8341 agatcgggca aagaagaggc gtttgaggca cttcgaaatc gattccccgg acttgcaccc
     8401 ctcactcaga cttcagcagg agaacagggc ggcgccgggc ctctcgaaca aaggacaaca
     8461 ggggttgcgc gcgatggagt gattctggtg ggagatgccg caggatatct ggacgcggcc
     8521 acaggagaag gaatcagcct tgccctggcc actgcacaat cgacggcccg gctcgcacaa
     8581 atgctacgaa aatcaaacaa gccgttgaac agactgcagc tgtcgggcta tgagaaggat
     8641 catcatcgca taacgtggaa ctatttcgtg atgacaaaga tgcttctgtt catgtcggat
     8701 catccgacgc tggctcgccc tttcattcgc atcatggcag tgcgccctcg ttctttccag
     8761 gctctactct ctttcaatat gggagagcct ttgtttccag tcctccggcg atttatcgcg
     8821 ggcctctttc gcaaataaga actttttaaa gaaggcgaaa gagtctgatc ttttctgcct
     8881 ttcctttcaa atcaaaaaaa cctgcgtctt caaactgagc cagcgcagac gcgcgatccg
     8941 caacactctg cgttaccagt atatccgttt tcgcttcttt ggtagccgat tctactctgc
     9001 tcgcaacgtt gacagtgtcc ccgatcacag tgtattccaa tctttctgca gttccaatat
     9061 tgcccgcaat cacctgtccg gtgtgaatcc ctattccctg tcgcagcgga agcagccctt
     9121 tcgattcacg attcgcattc atctgtgtga gagctcctcg catttcgaga gcagcggcaa
     9181 cggcacggtt cgtatcgtct ggcgcagtat cgggggtccc gaacgttgcc ataatcgcat
     9241 ccccgataaa cttatccagt gtgccattgt gtttgaaaat gatctgcacc atggtctcgt
     9301 gatattctct caaaaagcgt ataacgtctg ccgcaggcat tttttcgctc atcgcagtga
     9361 aaccgcgcaa atcggaaaat aaaaccgtta cttcttgcag tcgcccggat gatgtaaaaa
     9421 aggaatcttc cgcctgggcg atgtgattat acacggaagg tgaaaaatat ctgctgatct
     9481 gaagagccgc cttttcgcgg tgaatggcct caaacaatgt ctgacgagaa acgtgggtga
     9541 agaaagtcaa acaggcaccc acgccagcca taaccagcag attggtcgtg aggagcccgg
     9601 ggtggactct gtcgcccagc atgtgcgcta catagttttc ctcgaaaacc accctggaat
     9661 caaggccggc gtagtagaat agaaacaggt gatacgcaac cgcgcctatt gtggttgcag
     9721 ccggatacgc tggcctggac gcaacgctgt tcatgacaat gattgagtac gacatagtgg
     9781 taagggtatc gagcttcagc aaatacgcgc gcgaaacaac ggcacctccg ccaactgaat
     9841 tgagccatac gaaaggaagc acggcaatca taacaagatc aatcgctacg cctgcccagc
     9901 ccactacgct cattaaagac ggtcgccgaa ccaggtagaa ccctgagaga gtgcagattg
     9961 cgaagcttga gaataaacca attgtgacga acatttccca cctggtcgca ggcgtgaaga
    10021 atgcgagaat caaaccgaaa aaaacaaata cagagcgaaa cgttagaatg aatcgaagcc
    10081 cgcgtcgctc gcgcgtctca agcaatcgat ccagtgtctc actcaaacaa tcatgtcctt
    10141 cgcgcttcgt cttggagtga aatatttata gtccgatctg ccaatgcgaa caatcatctg
    10201 aatttttcca ggagcacgca cgcctgaaat cttttcaaac ttcgcacgca aatcatccat
    10261 ttccggaaat tcctgcaata tctgactgag aggatgcatt cgcaaccccg ccttcatcaa
    10321 tgcgagctgg aagcgagcgt aatctctgcc ggtctttacc cagtccacga tcgtattgga
    10381 acttgtagac caatagtaaa gagcacgtgc agaatcgatc ttctttttgt accgttcgag
    10441 aaacagcttg ttattcgcag gatcatggaa tttttccggt tcacgactca gaaagaatgt
    10501 ttctatcatc cagcgcgtaa atccagtgac tccctgatcc ggaagaccca gtccatctct
    10561 cttttcttga atctcacgat cgctaaatct gaaccagccg cgagtttcat ctgaaaggcg
    10621 atatgtcatg gtttcgagtt ccatggcaga attcatgaga ggaagataag gcttcatatc
    10681 ttccccgtag atcgccacac gctttgtata ccctgcaccc gcgtccgcct ccactttttc
    10741 tatttcagca ggagaaatca tagggccttc gtactctgcg cgattcgtgt gacgctcggc
    10801 gaccatggag tacagcgaat caggtgcaga tgcatccttg accaactgca cgtgcgcgac
    10861 aggcttcttg cctggttcta tactcgggta agtaccctct gggaaaagtt caatcttcg
//
LOCUS       DAOQJL010000058        10817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3133_length_10817_cov_5.730812, whole genome shotgun sequence.
ACCESSION   DAOQJL010000058 DAOQJL010000000
VERSION     DAOQJL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_10817_cov_5.730812"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLG78_04120"
     CDS             <1..484
                     /locus_tag="PLG78_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE22324.1"
                     /translation="RAFCQKMAEVNPNILDHSTIQRICKSATDIREIKSIFGKIELQS
                     RESKHLSTSNIDSVIEGTLRRPAIPSIHAILEAVTRHYHVSKQDILSSSRKTEHTWPR
                     HVAMYLALTQGGMSKSAIARFFKKKDHTTVVHAEKKIQIALENDDALAGALKAIVSTL
                     "
     gene            725..1837
                     /gene="dnaN"
                     /locus_tag="PLG78_04125"
     CDS             725..1837
                     /gene="dnaN"
                     /locus_tag="PLG78_04125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNE22325.1"
                     /translation="MKFTVDRQQFLKAITTVDGIIPSREIRSVYSNVLIEADKGEAVF
                     TTTDLEIALKTTLPITLDKKGGTTVPAKKLTQVIGAMDGETIEISLDDDKLIISSPKA
                     RTKPRVNLVTSPTSDYPPTPSPSSEDLISFSPAVFREMILKTYYSMAQDDSRYVFNGL
                     FLKSQKNSNIVVATDGRRLSKISRDFPSALPFKDGVIIPAKAIKELVRLLDAGEEAMI
                     AYDDKDKRVHFRIGRVDLVTKLIDGAFPDYEQVIPRKLDYKIRINRIDLEKAIKQAAS
                     VAIEPARQVRFIFSEGTVGFQASTPDLGGSESSLECEYSGPEMVIGFNSVYLMDVLKV
                     IPGEDVILGFSSPSSPCTVGDPADPDFLSVVMPMKI"
     gene            1847..2956
                     /gene="recF"
                     /locus_tag="PLG78_04130"
     CDS             1847..2956
                     /gene="recF"
                     /locus_tag="PLG78_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNE22326.1"
                     /translation="MRIEQLLLKNFRNYESLSILFPSRLTFLLGRNAAGKTNLLEAIG
                     MLSHGKSFRGASDHDLLRYGSNGYYIKAKYSRAGQKGEMEIATDVSTGDLRRKIKFND
                     KAASSRSSIVGEFICVVFSPSDLQIIEGSPADRRKFLDAAISSMEPEYLQNLIGYQKA
                     LRQRNALLKKIRQRQANIADLQIWNARLVQFASQLWSRRENFLLSFKEPFRDALEQIS
                     GNRDAMNLELQFSHRDDLKESYDKNVVRDLSLGYTTSGPHRDSLVFLKDGRDIMQFGS
                     QGQKRTAALALRIAQFHHLKQKLKITPLLLIDDVIRELDTMRRSAFVKLLRESGQAIF
                     TTPDLDGIDEDLKSLVSQSSVIHILGDGRVEQSVG"
     gene            2973..3266
                     /locus_tag="PLG78_04135"
     CDS             2973..3266
                     /locus_tag="PLG78_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNE22327.1"
                     /translation="MPQSAQLRELKNAFVRSLPFDEGTRELMLLGVSWDEIAGNLSAR
                     TRPEKIENEALYVSVAGGVYMQEFSFQSKTILKKVQNLIPSIRRIMIVQSRSG"
     gene            3259..3705
                     /locus_tag="PLG78_04140"
     CDS             3259..3705
                     /locus_tag="PLG78_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22328.1"
                     /translation="MGERTVLDGLRLQIETSGTETVYSGRIMLTFVTIAALALALIVF
                     LNALNLPTSAGAKKSPANIEKPVTLDTARILRMKAAERPRICPVCGTPLSQDEYLIAA
                     IFPDLGPNQKRQAHIYGCRHCYVSGGVNLTRVHPETLAEQPNVHSV"
     gene            3689..4582
                     /locus_tag="PLG78_04145"
     CDS             3689..4582
                     /locus_tag="PLG78_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22329.1"
                     /translation="MSTQFDLSHLKPEEIEKVRKVLLNLIAGIKARQVITAHGSALLK
                     DEEITLQSLLHPERGDVLVTIVRDHGMLHVFLSNRRDPGSPFSVMDYQTMRRFPARRP
                     VDSHHIVKDGETALFLCTQQDRDMLARESPDIISAYSSHFDVKEISELKTQAAPVDPI
                     SKSIQEKWESYRRHDPGDPAVAHIEKELFGYTITYAKYKASAREETVIIRPEKEKLFV
                     GQLIRDIYPYNIRAMFRKDNHEQHLQKLGEVHSTLRKLREQVTNRKGDSLPEPYMHYL
                     TGLQEMISNVLEKDPQLNGFV"
     gene            complement(4588..5301)
                     /locus_tag="PLG78_04150"
     CDS             complement(4588..5301)
                     /locus_tag="PLG78_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE22330.1"
                     /translation="MELTFLTYNIHRAIGFDRKYRLERIIETCRDVDADIVALQEVDQ
                     FAPRSKGEDLAHLIAQNLDMHYELGLNVRLQKGHYGNATFSRYPIEQAHNFNITWGIK
                     KKRGCLITSIGLPHAKIAIFNFHMGLARMEQVRQKKKIVHSDHMERLARVPMVLLGDT
                     NDRTTRLSHSFLESGFRDTCPGKGHNTFPAYAPILRLDRIFVTQQWEVVEHRVVRNKL
                     TRIASDHLPVVARLRLKSY"
     gene            5392..7098
                     /locus_tag="PLG78_04155"
     CDS             5392..7098
                     /locus_tag="PLG78_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE22331.1"
                     /translation="MITYRYGPYEPERDRPWDMDRLMNVLSDMIMRYDLELDEALRML
                     IDRGLPVNMFLKEGGMDDLVHSLQQQLQQKIDDVLGQFDIKPARKDTEFDLKKDSGDL
                     LNTAKSKEWKDRIEDAIRNLSIDELYRIKNEVMQEKKRPVSMDDIASVIQDMEDLDDL
                     VAGEKKYKFTGSTPLSRDEAIDILKHLDDLEKLMQALKEAEESGDLFNFNLEKLAQYL
                     GPEQFEEFLEKREQIFEKLRELLEKQGRIVPDPETGEMRLSPESVRKIGRRALEEIFS
                     KLKADTSGGMHSADETGESENVSSKTRPFDFGDPVSNLDLSSSIVNGYVRRKSSKPDL
                     SDMEVFEARGSARSSTVVLIDMSGSMMRSDRFYNAKRVVLALDALIRQDFKDDRLTIV
                     GFGTLASVIPPAKVPSLQPHPVTMYDPHIRLRYNMAAQTEKGKDRIPKYFTNLQRGLQ
                     LSRTLLGSGETKNKQIILITDGVPTAHFEGSSLHVNYPPSPADFEFALREVRNCTQDN
                     IVINTFLLTSDWEMGYFGEESFMRQFMKQSQGRVFYPHPTQMGKMVLVDFLQNKKAIF
                     QY"
     gene            7213..7506
                     /locus_tag="PLG78_04160"
     CDS             7213..7506
                     /locus_tag="PLG78_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNE22332.1"
                     /translation="MPYIITEPCQGVCDTACVDVCPVDCIHPRKDEWDKKGYSKDNLG
                     GKQLFIDPQECIDCGACEPACPVQAIFPEDEVPEKWKSFIGANYSHYGHTYKG"
     gene            complement(7575..8321)
                     /locus_tag="PLG78_04165"
     CDS             complement(7575..8321)
                     /locus_tag="PLG78_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNE22333.1"
                     /translation="MDEPMGLAAIALILFLGSTGVTAFASGFLFLPAKTQKSLLPILV
                     SFATGALLATALLVMIPDTLRQAGTQKTMQWVLIGILFFFLLERLVIFRHCHEDECST
                     HRAAGAVILTGDAIHSFFDGVIIASAFQYSVELGIATSISILIHEIPQEISDFAVLIQ
                     SGYTRNRALLMNYLAALPSVAGGILTFYFLSELRHLLPFFMSISAASLIYVAIADLVP
                     GLHRYAGLRTGLFHFLFLLAGVGVIAWIRG"
     gene            8393..8477
                     /locus_tag="PLG78_04170"
     tRNA            8393..8477
                     /locus_tag="PLG78_04170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8427..8429,aa:Ser,seq:tga)
     gene            8485..8573
                     /locus_tag="PLG78_04175"
     tRNA            8485..8573
                     /locus_tag="PLG78_04175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8519..8521,aa:Ser,seq:gct)
     gene            8599..9012
                     /locus_tag="PLG78_04180"
     CDS             8599..9012
                     /locus_tag="PLG78_04180"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNE22334.1"
                     /translation="MADKIREIQSRFSRNMQNNDSVAAVIGYIPLFGWIYPLFFRKEN
                     DFCQFHGKQAMHLNAGMLVVYFAVWILENFPITSWLFGADSWFHPITQAAWRITMLGY
                     FGLCAFGAYKAFIEERWEIPFLSDFVDRIQNQIKK"
     gene            9016..9393
                     /locus_tag="PLG78_04185"
     CDS             9016..9393
                     /locus_tag="PLG78_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22335.1"
                     /translation="MRARILSIPLHIRRVLYTGPFFVLWVIGYTVETSATELRAAGRG
                     LVTLIGFVASHAVILFVRELIRAFTVPAYPVEFTFFLLKSLIGLGYLGLTVWIAVREY
                     RNQETTLPFIDRLALRIDSVLSR"
     gene            9395..10456
                     /gene="corA"
                     /locus_tag="PLG78_04190"
     CDS             9395..10456
                     /gene="corA"
                     /locus_tag="PLG78_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNE22336.1"
                     /translation="MARLYRRDVARKRGAPPGTLVYTGDEKASTEIQLIEYDEERFQE
                     EVIKDPKVFQAYKDDPAVTWINVRGLSDVGPVVKMGEVFGLHPLVLEDILNTDQRPKF
                     EDYEKYLYIVFKNVTWDAKGEANYEQISLILGEDFLISFTESENDILKSLRDRLRTAK
                     GRVRRMGADYLAYCVMDLAVDNYFTVLEATGERLEILEESLLDPAANPSVREIQRMKR
                     MLLMMRKSVWPLREVVMKMERRESGLFKPGTEYFLRDLYDHTIQVVDTLETFRDMLQG
                     LMDIYLSTVSNRLNVVMKTLTVITTIFMPLTFLAGIYGMNFKHMPELEWTYGYPALIA
                     AMGMIAGTMYYWFRKRDWL"
     gene            complement(10457..>10817)
                     /locus_tag="PLG78_04195"
     CDS             complement(10457..>10817)
                     /locus_tag="PLG78_04195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE22337.1"
                     /translation="LLPHVPESDLPAVYSSALALIMPSLLEGFGLPALEAMACGTPVL
                     ASTGGSLPEVCGHAARFFDPRDEDAFIHAVQVVLQDSKQRARLRSLGLSQAAGFTWKR
                     NVDAMMRAYHHTGFEDS"
BASE COUNT         3015 a         2399 c         2685 g         2718 t
ORIGIN      
        1 cagagcgttc tgtcaaaaaa tggcggaggt gaatcccaac attctagacc attctacgat
       61 ccaaagaatt tgcaaatccg caacagacat tcgtgaaatc aaatccatct tcggtaaaat
      121 cgaacttcag tccagggaaa gcaaacatct ctctacctcc aatatcgatt ctgtcataga
      181 aggaaccctg cgaagaccgg caatcccgtc cattcacgcc attcttgagg cagtcactcg
      241 tcactatcac gtttctaaac aggacattct gagttcttct agaaaaacgg agcatacgtg
      301 gcccagacat gtggccatgt acctggctct cacccagggt ggaatgagca aatcagcaat
      361 tgcacggttt ttcaagaaga aggaccacac aacggtagtg cacgcagaaa agaagattca
      421 gattgctcta gaaaacgacg acgcactggc aggtgccctc aaggccattg tgagtacact
      481 gtgaacaatt gacaaacctg tcgtcagaaa gcaaagaatt tagttcaaaa tcaggtccgt
      541 tttcgcacag tgaatttctt tttggagtgt ttctgacagt tgtcttttta ttttgacata
      601 atcctttacg gacaggaaat cgttccgtcg cttgggaagc gagatttacg gcagtactga
      661 cttattcaca ggtacaacaa agtacaagta caaacacata atcattatat agttgaaggg
      721 caccatgaag ttcacagtag atagacagca gttcctgaaa gcaattacca ccgtagacgg
      781 tatcatccca tcacgcgaaa ttagatctgt atattcaaac gttttgatcg aagctgacaa
      841 gggcgaagca gtgttcacga ccactgatct ggagattgct ctaaaaacaa cgcttcctat
      901 tacactggat aagaaaggcg gaactacagt ccccgcgaaa aaactaacac aagtcattgg
      961 agctatggat ggagagacca ttgagatctc tttggatgat gacaaactca tcatttcttc
     1021 tccaaaagca agaaccaaac cgagagtgaa tctggtaact tctcctacct cagattatcc
     1081 tccaacacct tcaccttctt cggaagatct tatttctttt tcgcctgcag tttttcgcga
     1141 aatgattttg aaaacatact attcgatggc gcaggatgat agcagatatg tgttcaatgg
     1201 tttgtttcta aagtctcaga aaaattccaa tatagttgtt gcgacagacg gaagaagact
     1261 ctcgaaaatc tccagagact ttccatctgc acttcctttc aaagatggcg tgatcattcc
     1321 ggcaaaagca atcaaagagc ttgtccgcct cctggatgca ggcgaagaag caatgatcgc
     1381 atacgatgac aaagacaaac gtgttcattt tagaatcggt agagtagatc ttgtaacaaa
     1441 gctaattgat ggagcatttc ctgattacga acaagtcatt ccacgaaagt tagattacaa
     1501 aatcaggatc aatcgcattg acctggaaaa agcaatcaaa caggcggcgt cagttgccat
     1561 agagcctgct cgccaggtta gatttatttt ttctgaaggg accgttggtt ttcaagccag
     1621 cacacctgat ctggggggaa gtgaaagcag tctcgaatgc gaatactccg gtccggaaat
     1681 ggtgattgga tttaattccg tttatctaat ggatgttcta aaagtaattc cgggtgaaga
     1741 tgtgattctt gggttttctt ctcccagttc gccgtgcaca gtgggagatc cggcggatcc
     1801 agatttctta tctgtggtta tgccaatgaa gatttgatca tcgcttgtgc gaattgaaca
     1861 actcttactg aagaatttta gaaactacga atcactatct attttattcc cgtctcgctt
     1921 aaccttcctt cttggaagaa atgcggccgg gaaaaccaat ttgctcgaag ccattggaat
     1981 gctttcgcac ggaaaatctt ttcgaggagc ttctgatcac gatttgctcc gatacggatc
     2041 caatggatac tatataaaag cgaagtattc tagagcaggc cagaaaggag aaatggaaat
     2101 tgcaacggac gtttccacag gtgatttacg gcgtaaaata aaatttaacg acaaagcggc
     2161 ttcttcgaga tcatcgatag taggagaatt catctgtgtt gtcttttctc cttcagattt
     2221 acaaatcatt gaaggttcac ctgcagatcg aagaaagttt ttggatgctg cgatttctag
     2281 catggaacca gagtatctcc aaaacttgat cggatatcaa aaagcactca gacaaagaaa
     2341 tgccctattg aagaagatca gacaaagaca ggcaaatatc gcagatttgc agatctggaa
     2401 tgccagactg gttcaatttg catctcagct ctggagcaga agggaaaatt ttcttctttc
     2461 attcaaggaa ccgtttcgcg acgctctgga acaaatatcc ggaaacagag acgcgatgaa
     2521 tcttgagctt cagttttcgc acagagatga tctcaaagaa agctatgata agaacgtggt
     2581 tcgcgatcta tcactgggat acacgacaag tggtccgcac agagactcgc tcgttttctt
     2641 gaaagatggt cgtgatatca tgcaatttgg aagtcaggga cagaagagaa cggcggcact
     2701 tgcactcagg atcgcacagt ttcatcattt gaagcaaaaa ctaaagataa cacctttgct
     2761 tctcattgat gatgtaattc gagaacttga tacaatgcgc agatcggcct ttgtgaaact
     2821 cctgcgcgaa agtggccagg cgatttttac tacaccggat ctagacggga tcgatgagga
     2881 ccttaaatct ctggtcagtc agagttccgt aattcatatt cttggggatg gcagggttga
     2941 acaatccgtt ggatgaagag caggctcctg gtgtgcccca gtctgcgcaa ttgcgagaac
     3001 tcaagaacgc attcgtgcga tctcttccgt ttgatgaagg gacgcgtgaa ttgatgcttc
     3061 tgggagtatc gtgggacgaa attgcaggaa atctgtctgc ccggaccagg cctgaaaaaa
     3121 tagagaatga agccttgtat gtgagtgtcg cggggggcgt ctatatgcag gaattttcat
     3181 ttcaaagcaa gaccattttg aaaaaagtcc agaatctaat ccccagcatc cgcaggatta
     3241 tgatcgtcca atccagaagt gggtgaaaga actgttctgg acgggctcag gctccagata
     3301 gagacctctg ggaccgaaac agtctatagt ggtagaatca tgctaacatt tgtaacgatt
     3361 gcggcactgg cccttgcact catcgtcttc ctgaacgcgt tgaatctgcc cacgtctgct
     3421 ggcgcgaaaa agtcgcccgc caatattgag aaacctgtga ctctggatac ggcacgtatt
     3481 ctgcgtatga aagcggcgga acgtcctcgg atatgtcccg tctgcggaac acctctcagt
     3541 caggacgaat atctaatcgc tgcaatcttt cctgatctcg gtccaaatca aaagcgacag
     3601 gcacacattt acggatgccg ccactgctat gtgagcggtg gagttaactt aacccgggtt
     3661 catccggaaa cactggcgga gcaaccgaat gtccactcag tttgatctat cgcacctgaa
     3721 accagaggaa atcgaaaagg ttcgtaaagt tctactgaac ctgatcgcgg gaatcaaagc
     3781 cagacaggtc atcactgcgc atggaagcgc gcttctaaag gatgaagaga ttactctcca
     3841 atcgcttttg catcctgagc ggggggacgt actggtaact attgtgcgcg atcacggaat
     3901 gttgcatgtg tttctctcaa atcgcaggga tccaggaagt ccgttttccg ttatggatta
     3961 tcaaaccatg cgacgattcc cggcgcgtcg cccggtagac agtcatcaca ttgtgaaaga
     4021 cggagagacg gcactttttc tttgcacgca acaggatcga gacatgcttg cgcgtgaatc
     4081 gccagacatc atcagcgcgt actcaagtca ttttgacgta aaagaaattt cagaattaaa
     4141 gacgcaggct gctcctgtag atccaatcag caaatccatt caggaaaaat gggaatcata
     4201 tcgcagacat gatccgggag atccggctgt ggctcatatt gaaaaggaac tgtttggata
     4261 cacgattacg tacgcaaagt ataaggcgtc tgctcgcgaa gaaacagtga tcattcgtcc
     4321 ggagaaagag aagctctttg ttgggcaatt gatacgtgat atctatccgt ataacattcg
     4381 tgcgatgttt cgcaaagaca atcatgaaca gcatttgcag aaactcggag aggtgcattc
     4441 cactctgcga aaactaagag agcaagtaac caatcgaaaa ggtgattcgc ttcctgaacc
     4501 atacatgcat tatctaaccg gtcttcaaga gatgatctcg aacgtacttg agaaggatcc
     4561 gcaactgaac ggatttgtat aatcagatca gtaagactta agtcttaagc gtgccactac
     4621 gggtagatga tcggaagcga ttcgagtgag tttgtttcgc acgactctgt gctccactac
     4681 ttcccattgt tgagtgacaa agattctatc gagtctgaga attggagcat aagcgggaaa
     4741 agtattgtga ccctttcctg ggcatgtatc gcgaaatcca gattcaagaa atgagtgaga
     4801 aagtctggta gtccgatcgt ttgtatcacc aaggagaacc atgggaactc gagcgaggcg
     4861 ctccatatgg tctgaatgaa cgattttctt tttctgtcta acctgctcca tgcgcgccag
     4921 gcccatgtga aaattgaaga tagcgatttt ggcatgaggc aagccaatgc ttgtgattag
     4981 acaacctcgt ttcttcttaa tgccccaggt tatgttgaag ttgtgggctt gttcaatagg
     5041 atatcttgaa aaagtggcgt tgccgtagtg gcctttctga agcctcacat tcagtccgag
     5101 ctcatagtgc atgtctagat tttgcgcaat cagatgcgcc aggtcttcgc ctttgctgcg
     5161 aggggcaaat tggtccactt cctggagggc gacaatgtcc gcatccacgt cgcggcaggt
     5221 ttctatgatc ctttcgagcc tgtatttgcg atcgaatcca atggcccgat ggatattgta
     5281 ggtcagaaaa gtaagttcca ttctgccggc cgggtgctat cctgttattt gttgctgacg
     5341 actgacgatg gcatctggta agagaccgac tggaattgga agcaaatttg tatgatcact
     5401 tacaggtatg gcccatacga acccgaacgt gaccgcccct gggacatgga tcgtttgatg
     5461 aatgtccttt cggacatgat catgcgatac gacctggaat tggacgaagc tctcagaatg
     5521 ctcatcgatc gtgggttgcc ggtcaatatg tttctgaagg aaggcggtat ggacgatctg
     5581 gtccatagtc tccaacagca attgcaacag aagatcgatg atgtgctcgg tcaatttgac
     5641 attaagccag cgcgaaagga cacagaattt gatttaaaaa aagattctgg cgatctgcta
     5701 aatacggcga agagcaaaga atggaaggat agaatcgaag atgcgattcg caacctgtcc
     5761 attgatgaat tgtatcgcat caagaatgaa gtgatgcagg aaaaaaagcg tccggtttct
     5821 atggacgaca tagcatccgt catccaggac atggaagacc tggatgatct cgtggcagga
     5881 gaaaagaagt ataagttcac aggctccact cccctttcgc gcgacgaagc cattgacata
     5941 ctcaagcatc tggatgactt agaaaagcta atgcaggctt tgaaagaagc agaagaaagt
     6001 ggtgacctct tcaattttaa cctggagaag cttgctcaat atctcgggcc cgaacagttt
     6061 gaggagtttc tagaaaagag agaacaaatc tttgaaaagc tcagagagct tttagaaaaa
     6121 cagggaagga ttgttccgga tccagaaaca ggggagatgc gtctttcgcc tgaatcagtg
     6181 cgaaagattg gaaggcgtgc gctcgaggaa atcttcagta aactaaaagc ggatacatcc
     6241 ggcgggatgc attctgcgga tgaaacagga gaaagcgaaa acgtttcgag caaaaccagg
     6301 ccatttgatt ttggagatcc agtatccaat ctggacttgt cttcttcgat cgtcaacggc
     6361 tacgttaggc gaaagtcaag caagccggac ctctctgata tggaagtatt tgaagcgcga
     6421 ggatctgcta gaagctccac tgtagttctg attgatatga gtggaagcat gatgcggtcg
     6481 gatcgttttt acaatgcgaa acgcgttgtg ctggctctcg atgctttgat tcgtcaggat
     6541 ttcaaagatg atcgcttgac gatcgttggt tttgggactc tagcaagtgt aattccgcca
     6601 gcaaaagttc cgagtctcca gccacatcca gtgaccatgt acgatccgca cattcgtttg
     6661 agatacaaca tggcggctca aacagaaaag ggtaaagata gaattcctaa gtattttacg
     6721 aacctgcaac gggggctgca gttatcgcgt actctactcg gaagcggaga gacaaagaac
     6781 aagcaaatca tcttgatcac agatggggtg cctacggctc attttgaagg cagcagtctg
     6841 catgtaaatt acccgccttc acctgcggat tttgaattcg cactgcgcga agtgcgcaac
     6901 tgcacgcagg acaacattgt catcaacacg ttccttttaa cgtctgactg ggagatgggc
     6961 tattttggcg aagaatcgtt catgcgccag tttatgaaac aatctcaggg tcgggtcttt
     7021 tatccacatc ccacgcagat gggcaagatg gttctcgtgg attttctaca gaacaaaaag
     7081 gccatttttc aatactgagg gttgccgttc ctttccgttg catgagcctc actccccacc
     7141 ctttgtgaaa aaacgtaggt gcggcgataa aaaaaactgg aagggctcgg accgcataaa
     7201 taagaggtct gtatgccata cattatcaca gaaccctgcc agggcgtgtg cgatacagca
     7261 tgcgtagatg tctgcccggt tgattgcatt cacccacgta aagacgaatg ggacaagaaa
     7321 ggctattcca aagacaacct cggcggaaag cagctgttca tcgatccgca ggagtgcatt
     7381 gattgtggag cctgcgaacc cgcctgcccg gtgcaggcaa tttttcctga agacgaagtt
     7441 ccggaaaagt ggaagtcatt cattggtgcg aactattctc attacggcca tacttacaag
     7501 ggctaaagag agtgtcttgg caatgccggc gtagaatccg gcattgcttc tgattttcgc
     7561 gccccctctt tccatcaacc gcgaatccag gcaatcacgc ccacgcctgc gagcaaaaac
     7621 agaaagtgaa ataatcccgt tctcaatccc gcataacggt gaagtcccgg caccaggtcc
     7681 gcgatggcta catagatcaa gctcgcagct gagatcgaca tgaagaatgg aagtagatgc
     7741 ctgagttcag atagaaaata gaatgtgaga attccacctg ccacagacgg aagggccgcc
     7801 aggtagttca tcaaaagagc gcgatttctt gtatatccgc tctggatcag cactgcgaaa
     7861 tcgctgattt cctgcggaat ctcatgaatt agtatggaga tgctcgttgc gattccaagc
     7921 tcaaccgaat actgaaaagc ggaagcgatg atcacgccgt caaagaagct gtgaatcgca
     7981 tctccagtca ggatgaccgc tcccgctgcg cgatgagtgg agcactcgtc ttcatggcag
     8041 tggcggaaga ttacaagtct ctcgaggagg aagaagaaca aaatgccgat caaaacccac
     8101 tgcatcgttt tttgcgtacc agcctgacgc aaagtgtctg gaatcatcac caggagtgct
     8161 gtcgcaagta gcgcgcccgt ggcgaaactg acgaggatgg gcagaagcga tttctgggtc
     8221 ttcgcaggta gaaatagaaa gcctgaagcg aacgcagtga cgcctgtcga accaagaaac
     8281 aaaatgagcg caatggcagc gaggcccatg ggttcatcca tttgttgcat gagttgatat
     8341 tttgaaagtc tttctagttg acactcgcct gaaaagtacc agcctggaaa gtggagggat
     8401 ggccgagtgg tcgatggcgg cggtcttgaa aaccgttgtg cagagatgta ccgtgggttc
     8461 gaatcccact ccctccgggg aattggagag gtggctgagt ggccgaaagc ggcagtttgc
     8521 taaactgttg tacgagttaa atcgtaccgc gggttcgaat cccgccctct ccgaaatttc
     8581 gaggccttcc aggaagtcat ggctgacaag attagagaaa ttcaatcgcg gttttcgcga
     8641 aacatgcaga acaatgattc tgtcgcagcg gtgatcggat acattccgct ctttggttgg
     8701 atctatccgc tcttttttcg caaggaaaat gatttttgcc agtttcacgg taagcaggca
     8761 atgcatttga atgcaggcat gctggttgtt tacttcgcag tctggattct cgaaaatttt
     8821 cccattacgt cgtggttatt cggcgcagat tcatggtttc atccaatcac gcaggcggcc
     8881 tggcgtatta ctatgctcgg atatttcggg ctctgtgcat ttggcgcata caaagctttc
     8941 atcgaagaga gatgggagat cccgtttttg agcgattttg tggatcgcat ccagaaccag
     9001 attaagaagt aaatcatgcg cgctcgaatt cttagcattc ctttgcacat ccgtcgagtc
     9061 ctttacacag gtcctttttt tgtgctctgg gtaattggct acacagtgga aaccagtgcg
     9121 acagaactca gggcagccgg gcgtggcctg gtaacattga ttggttttgt tgcttcgcac
     9181 gcagtgattc tatttgtgcg agagctcatc agggctttca ctgttccggc ctatcctgtt
     9241 gaatttacat tctttctact gaaatccctg atcggcctcg ggtatctggg actcactgtc
     9301 tggattgcag tacgcgaata cagaaatcaa gaaaccactt tgccattcat agacaggctt
     9361 gcgcttcgca tcgattccgt tctgagcagg tagtatggct cggctttata gacgagatgt
     9421 agcccggaaa agaggagctc ctcccggaac tctagtctat accggggatg aaaaagcttc
     9481 tactgagatt caactcatcg agtatgatga ggaaaggttt caagaagaag tcattaaaga
     9541 tcctaaagta tttcaagcgt acaaggatga tcctgccgtc acctggatca atgttcgtgg
     9601 tttgtcggat gtgggcccgg ttgtaaaaat gggtgaggtg tttgggctgc atccactggt
     9661 gctggaagac attctgaata cggatcaaag gcccaaattc gaagactacg aaaagtatct
     9721 ctatattgtg tttaagaacg tgacgtggga tgcgaaaggt gaagcgaatt acgagcaaat
     9781 cagcctgatt ttaggggaag atttcctcat ttctttcaca gaatccgaga atgatattct
     9841 aaagtcgctt cgagatcggc ttcgcacagc caaaggccgc gtgagacgaa tgggtgcaga
     9901 ctatctggct tattgcgtca tggatcttgc cgtggataac tatttcacgg tgcttgaagc
     9961 cacaggcgag agactggaaa tacttgaaga gagtcttctg gatcctgcgg caaacccatc
    10021 cgtgagagaa atccaaagaa tgaagcgaat gcttctgatg atgcgcaaga gtgtctggcc
    10081 tttgcgcgaa gtagtcatga agatggagcg tcgcgaatcc ggtctattca agcctggcac
    10141 ggaatacttt cttcgtgatc tctatgatca caccattcag gttgtggata cactggagac
    10201 atttcgcgac atgttgcaag gcttgatgga tatctatctt tctactgtga gcaatcgtct
    10261 caacgtagtg atgaagactc tcactgtgat tacaaccatc ttcatgcctc tcacgttcct
    10321 tgcgggaatt tacggaatga atttcaaaca tatgccagag ctggaatgga cgtatggcta
    10381 ccctgcattg atcgcagcga tggggatgat tgcaggcaca atgtattact ggtttagaaa
    10441 aagagactgg ctctagtcag gaatcttcaa atccggtgtg atgatacgct cgcatcatcg
    10501 cgtctacatt tcgcttccac gtaaatccag cggcctgcga taaaccaagg gatcgtagtc
    10561 gtgcgcgctg tttggaatct tgcaacacaa cctgcactgc gtggatgaat gcgtcttcgt
    10621 cccgcggatc aaagaagcgc gctgcatgac cgcatacttc cggaagactt ccgccagtgg
    10681 atgcaagaac tggagttcca caggccattg cttctagagc aggcagtccg aatccttcaa
    10741 gcagagaggg catgatgagc gccagagcag aagagtatac ggcaggaaga tctgactcag
    10801 gaacatgagg gagaagg
//
LOCUS       DAOQJL010000059        10771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3153_length_10771_cov_4.745521, whole genome shotgun sequence.
ACCESSION   DAOQJL010000059 DAOQJL010000000
VERSION     DAOQJL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3153_length_10771_cov_4.745521"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLG78_04200"
     CDS             <1..332
                     /locus_tag="PLG78_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22338.1"
                     /translation="MSAATMSGTTAVSVVKDVSLNTCNFRRSTFGVSGSNSNGLTCPS
                     CTNSTNVKRMILLNGMLIAVGTMGACSGNPPFVMRSTDAGMTVSSDTIPSSIPSNGIL
                     NDIIAVY"
     gene            335..1411
                     /locus_tag="PLG78_04205"
     CDS             335..1411
                     /locus_tag="PLG78_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22339.1"
                     /translation="MKQRILALVFSALCCCRYSDRPDTTWLSSLAFAAPWMMIATGTN
                     CTGWYSVNGRTWQSFLFPGCTTGTISSIAYGAGAFVAVGSTNGTACGIWTSTGKYTPQ
                     WTLQSCGPTSARMSAVAFGSGFAGNEFVAAGVATGANFQSVSSMDAGVTWVDATITEA
                     GSGAAVGSLVYWPTGQLFIESSGTPQNTRRRFIGGGTTWIDGDNLGVLNPKIATGPTL
                     ANGSVRVYDAGNSPTVIQYGDDAFVGAPTNLSPNVFGSALPVVNDMIYGEGKRMVFVR
                     DTCGVTFTSSSSAGDASGSYTMSNCVSNLKAVGYLDPFFIAGTDDGSFYYSVSGLPSE
                     WSRASSASSTSVVQRMAGRWGPFN"
     gene            1457..2290
                     /locus_tag="PLG78_04210"
     CDS             1457..2290
                     /locus_tag="PLG78_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22340.1"
                     /translation="MKLRILLVAFSISVATLFAQDASRLNKLIDGQRNAFESKKKALA
                     LIESGASLLKEQKYKDAASSYLAALELDPSNSAAYFSFATALSGSGRIDESRRILTVL
                     FEFATPEEKSAMNSKFGAERWPGGAFAGFSSAYSKQTLAGNGWGAAPDDLEGIVTKKE
                     SGFYTLVHAKASEDAIRGNSAGMMQSTCRRSATLVGATSVLESLARSALREAKKENGT
                     VTMGSYSCTIANGSASCSGVVRGHSILECSGRGPGGSYAECDCTVYLPVQQGKTSVKV
                     D"
     gene            2341..2754
                     /locus_tag="PLG78_04215"
     CDS             2341..2754
                     /locus_tag="PLG78_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE22341.1"
                     /translation="MVSGKSKEVEAYLKLFSSSEQALLEELRQIIHSVGKFEECISYK
                     MPAFRYKEGIAAGFLLYRHHIGFYPFSGATLRNFKEDIKSYKSTPGALQLSREKKLPR
                     GLIKKIVRARMKEIDQKSTGKTAKSVSGRGRSAKS"
     gene            2801..3091
                     /locus_tag="PLG78_04220"
     CDS             2801..3091
                     /locus_tag="PLG78_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22342.1"
                     /translation="MNEANPETLRKALLSKISPGTSLASAKDKMVEEGFVCTEMKNSS
                     FQSDRLHSGIDYLYCDRSICGSSMVTRRDQVAVVHQRGVVTEILVSTGLVGP"
     gene            3133..3816
                     /locus_tag="PLG78_04225"
     CDS             3133..3816
                     /locus_tag="PLG78_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22343.1"
                     /translation="MFIGHYAASFALKKVSPRTSLRALLLGVQFVDILFMIFILFGIE
                     RMRLIPGTTASNSLKLDFMPYTHSLLGTFLWAVGAFVLFRFALLRKSGHENHTRNLIS
                     FAVAIAVLSHYFFDVPMHLRDLPLAGDDSYKLGLALWNNRIAAVSLEFFLTLGMFAVY
                     LTASKPGQGFAGKYGMPIFGAALLVICVLQYIIPPPTTIIELSSQALASYIGIGLVGG
                     WLDQKRVYS"
     gene            complement(3820..4083)
                     /locus_tag="PLG78_04230"
     CDS             complement(3820..4083)
                     /locus_tag="PLG78_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22344.1"
                     /translation="MRTVIASLLFIAGVPIFLFGFVFLLKETYRDHGLLWAILLVTCF
                     PGVILVLSLGRSKSFLQSWVFPFPWSFVWLGLLLISCATWIAG"
     gene            complement(4114..5109)
                     /locus_tag="PLG78_04235"
     CDS             complement(4114..5109)
                     /locus_tag="PLG78_04235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22345.1"
                     /translation="MPALVTGASGHIGSNLVKQLLDQGEKVRALIRKSSNTACLPDHK
                     NLEIVYGDVYDRPSLVAAVTGMPFVYHTAAVYSLSRKNPGQILETAMQGTRNILEAVK
                     VAGGVKRVVYTSSVAAVGMTDDPTRPLDESNWTADSDPDEYTRAKLESEKLAHSLARE
                     FGIDLVAVNPALVLGALDLKPTPSNKVVLDFLQGKVPAYLETGFSAVSVEDVASGHIL
                     AMQKGRSGERYILSGENVKLVDFFEILGKISGKKPPGIKLPRPLAIGMAAMFEFAAGI
                     TKSEPLATVQAMKSITNRYGFYSFARAGKELSYKPRPLDQTLRSTVEWFEKNPLT"
     gene            complement(5157..6113)
                     /locus_tag="PLG78_04240"
     CDS             complement(5157..6113)
                     /locus_tag="PLG78_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE22346.1"
                     /translation="MKVFVTGGSGFVGERLIRRLKADKHEVVALARGSSQAEKIKAMG
                     AQPVHGTFSNISEWETALDGIDAVVHCAAPVEFWGKWEDFYRDTVQATIDLYQAASRR
                     KVGRFIQISSESVIQDKLPLLDIDETAPYPSVPSSVYGRAKKETEIALCAQPTGPVRI
                     ILRPTFIWGKGVKALDSMAQKVTSGEFMWIDHGQTIIEMVHVDNVVQAIVLSLGAGSD
                     KSIYYVTDDRPMKVFDFFSALFAAAAIPIPKKNMNGKIAALAAGAIEGVWKLFGIQKS
                     PPLSRFELAFVAQPRRYNIAKIKRELGYSPVKTLEAGLAEMR"
     gene            6191..6895
                     /locus_tag="PLG78_04245"
     CDS             6191..6895
                     /locus_tag="PLG78_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE22347.1"
                     /translation="MATKKATKKRMPAAARRQLILESAMQLFAKKGFHGTTTADLARV
                     AGVSEALIFRFFPDKESLYREVQFLCVEARGAADSMLDQARPGTENLVRAVLILIYEV
                     FSGFGGRTKNETLRRLQTHSMLEDGKFARAFFKSHFKVYFPFIDRCLKSAHRSGDLEP
                     GGMPLECVIWFTHHLPMMVRISILSHRADYFASSDAELLESMALFCLRGIGLKSSAIK
                     KNLPRNWTEQFNLLKG"
     gene            6921..7916
                     /locus_tag="PLG78_04250"
     CDS             6921..7916
                     /locus_tag="PLG78_04250"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNE22348.1"
                     /translation="MRATVLGSGSFGTALASVLADKNAEVWIWSRSPDMARAIHEQHE
                     NTKYLPGFRLADNIKSSPDMAECLKDTDLIVFGIPSQSLTQVLKENTHILPEGVPIVS
                     AAKGVDRATLRLVSEIFEEELPGKFHKYLSYLSGPSFAKEILKQMPTVVSIASRNEEI
                     AKQVQQMFAHTWFRTYWTPDVTGVEVGGAIKNVIAIAAGVSDGLGLGHNTRAALITRG
                     LAEITKLGVRKGADPLTFLGLAGLGDLVLTCTGDLSRNRTVGFKLGQGMKLQQILTEM
                     NQVAEGVVTTESAHLLAQKLGIEMAITDQVYQLLYQDKDPKQVVRDLMTRDLKRE"
     gene            7977..8822
                     /locus_tag="PLG78_04255"
     CDS             7977..8822
                     /locus_tag="PLG78_04255"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE22349.1"
                     /translation="MIQFDESKIPPLPAVAARVMQFDPGSPNSSIATLERIIAPDQGI
                     SAELLRVSNSAYYGRSGSIKTLRDAITLLGLKTVKSMVMLICTRTFTQKIRGDLLRKY
                     CIEFPIVTALLCIDNARAANHKDLQEESFLCGLLHRIGMTMLAVEKKSEYAELIARAE
                     KEGASLVDLEKDAFGMTHDDINALVIKKWNLPEITVRGILDCEFPAEATNKVSHHAKI
                     TALSRTIASQLLDIPRPPAEMERANAVIKAYGLKADLFSHYTPDYFDLLKENPFYQSA
                     VAVIG"
     gene            8800..9897
                     /locus_tag="PLG78_04260"
     CDS             8800..9897
                     /locus_tag="PLG78_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22350.1"
                     /translation="MRWQSLDEEQKRPRVVLVEDDLVQREMIAQMLIFAGYDVTSAAT
                     GEEGQELIESVKPDAAVVDLHLPAERGVTGLVLLKSIKENPETAELPVLIITADTTEE
                     TYVLVLSSGASDILLKPLRITELTIRIQNVLELRKNRADLREANAKLEREKKRLLRYF
                     SEDFVTAMLNDEISAELGGISVDATILFMDIRNSTGIAEQIGPQKFAEFVSMIFTDVM
                     DLIFANRGSVNKLLGDGILATFGCPVPSEKDAENAVKTASQIREYFQVINEARAHEDI
                     GGDIGFGIGVASGKVFAGNIGSFRRMEYAVMGDPVNLAARLQELSKTLSVDIVIDGTT
                     KSKIPASYAVRDSGVKNVRGRHGEVEAWILS"
     gene            complement(9904..10353)
                     /locus_tag="PLG78_04265"
     CDS             complement(9904..10353)
                     /locus_tag="PLG78_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22351.1"
                     /translation="MRRILPCFALILGACTNYSAETQNVNPPILTSIAREGTGHIITM
                     AAQNTEIGFAGYRLFQGTTDTGVRTANSLSGIDCSRPLAVLPNQAISYVIEVKPGQTA
                     VSAGYSNRVCAVPLALTAGTLVGVRSLILRDLLSLDTGLSSNTLVVP"
     gene            complement(10350..>10771)
                     /locus_tag="PLG78_04270"
     CDS             complement(10350..>10771)
                     /locus_tag="PLG78_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22352.1"
                     /translation="AAGFDEVVATEVVLEERMPLFPRIHSKNNRGIEKLLRMPEICGE
                     SLIESIRTDYERLSKNPSLFDARPIANSTGYSDSSADLPLLRICEKKTLVHPSKNLEE
                     LGKAQSWRILRPRRPYSGKIEKYVRLCLCLVGLLRLQ"
BASE COUNT         2583 a         2620 c         2826 g         2742 t
ORIGIN      
        1 cgatgagcgc ggccaccatg tccggcacca ctgctgtgtc ggtggtgaaa gatgtgtccc
       61 tcaatacgtg caatttcaga cgcagcacat tcggtgtgag cggatccaat tcgaacggtc
      121 tgacgtgccc aagctgtacc aactcaacca atgtgaagag aatgattctt cttaacggca
      181 tgttgattgc cgtaggcacg atgggtgcgt gttccggcaa tccgccgttt gtgatgcgct
      241 ccactgatgc agggatgaca gtttcctctg acaccatccc atcctcgatc ccgtccaacg
      301 gcatcctcaa cgatatcatc gctgtttatt gactatgaag caaagaattc tcgcgctcgt
      361 tttctctgcg ctttgttgtt gcagatattc cgatcgacct gacaccacgt ggctctcgag
      421 tcttgcgttc gccgcgccct ggatgatgat tgcaaccgga accaattgca ctggctggta
      481 ttctgtgaat gggcggacgt ggcaaagttt tctttttcca ggatgcacta ccggaacgat
      541 cagtagcatc gcctacggag cgggggcgtt tgttgccgtt ggttccacaa acggaactgc
      601 atgcggtata tggacgagta caggaaaata tactccgcag tggactctgc aatcttgcgg
      661 tcctacttct gcacgaatga gcgctgttgc gtttggctct ggatttgccg gaaatgaatt
      721 tgtcgcggcg ggtgtcgcca ctggtgccaa ctttcaatct gtttcttcca tggatgcggg
      781 ggtaacgtgg gtggatgcaa cgattacgga agccgggtcc ggggccgcag tggggagcct
      841 tgtctactgg cccaccggac aattgttcat cgaaagttca ggcactccgc agaatacgcg
      901 aagacgcttt atcggaggcg gaactacctg gatagacggt gataatctgg gtgtactgaa
      961 tccgaaaatc gcaaccggcc cgacgcttgc gaacggaagc gtgcgcgtct atgacgcagg
     1021 caattctccg acagtgatcc agtacggaga tgatgcgttc gttggtgcac caaccaattt
     1081 gtcgcctaac gtctttggat ccgcgcttcc tgtcgtaaat gacatgatct acggcgaagg
     1141 aaagcgcatg gttttcgttc gcgacacatg tggcgttaca tttacatctt cgagcagtgc
     1201 cggggatgca agtgggtctt atactatgtc caattgcgtt tccaatttga aagcggtggg
     1261 ctatcttgat ccgttcttca ttgcaggaac agatgacgga tcgttctatt attccgtaag
     1321 cgggcttccg tcagaatggt ccagagcgtc aagcgccagt tctacttccg tggttcagag
     1381 aatggcaggt agatggggac cctttaactg agttttttga tcgaacaata cgacagcact
     1441 cgtagtccat gacactatga aactcagaat actcttagtt gcattttcca tttcagtggc
     1501 tacactgttt gcccaggatg ccagtcgttt gaacaaactg atcgatggcc agagaaatgc
     1561 tttcgaatca aaaaagaagg cactggctct aatagaatct ggtgcgtcct tactcaagga
     1621 acagaaatac aaagacgccg catccagtta cctcgcggca ctggagctgg atccttctaa
     1681 ttccgccgct tatttcagtt ttgcaactgc attgtcgggc tctggcagaa tcgacgaatc
     1741 gcgcagaatc ctgaccgttt tgtttgagtt cgcaactcca gaagagaaaa gtgccatgaa
     1801 tagtaagttt ggcgcagagc gctggcccgg aggagctttt gctggttttt catccgcata
     1861 ttccaaacag acgttagccg gcaatggatg gggtgcggcg ccggatgacc tcgaagggat
     1921 cgtaaccaaa aaggagagtg gcttctacac actcgtgcac gcgaaggcga gcgaagatgc
     1981 gatccgcgga aatagtgccg gcatgatgca gtccacttgc agaagaagcg caactcttgt
     2041 aggcgccact tccgttcttg aatccctggc tcgctctgct ttgcgcgagg ctaagaaaga
     2101 gaacgggact gttaccatgg gttcgtatag ctgcacaatt gcgaacggtt cggcttcctg
     2161 cagcggcgtg gtacgaggcc attccattct agagtgttcg ggacgggggc cgggcggatc
     2221 gtatgccgag tgcgattgta cagtttatct gcccgttcaa caggggaaga cgtcagtgaa
     2281 ggttgattga ttgcgtcaaa ttattcttgc ttgtttggcc gacccatcgc ggtctaggga
     2341 atggtttcgg gcaaaagcaa ggaagtggaa gcatatctga agttgttttc gtcttctgaa
     2401 caagctctcc ttgaagaatt gaggcagatc attcactccg tcggcaaatt cgaagaatgc
     2461 atttcgtaca agatgcctgc cttccgctac aaggaaggaa tcgccgccgg gttcctcctg
     2521 tatcgccatc atataggttt ctatccattc agtggtgcga ctctgcgaaa tttcaaagag
     2581 gacatcaagt cctataaaag tacaccagga gctctccagc tttcgcgcga gaagaagctg
     2641 ccgcgagggt tgatcaagaa aattgtgcgg gcaagaatga aggaaatcga tcagaaatcc
     2701 acaggtaaaa ctgcgaaatc tgtatctggc cgaggtcgaa gtgcgaaatc ttaatccgct
     2761 cttgcttgct ggattattgc tgttttcgtg tcgatcccaa atgaacgaag ccaatccaga
     2821 aactttgcgc aaggctcttc tttcaaagat ttcccctggc acgagtctcg cgtctgcgaa
     2881 agacaaaatg gtggaggaag gattcgtttg cacagaaatg aagaattcat cttttcaatc
     2941 agacaggtta cattcaggaa tcgactattt gtattgtgat cgcagcatat gcggtagcag
     3001 catggttacg cgaagagacc aggttgcggt cgtgcatcag cggggagtgg tgacagaaat
     3061 actcgtttct acagggcttg ttgggccgta agttattact tgcctgattt gcgggccaca
     3121 gtatggtccg gcatgttcat tggtcactat gctgcgtctt tcgcactgaa aaaagtttct
     3181 ccccggactt cgctgagagc gttgctgctg ggggttcaat tcgtagacat actgtttatg
     3241 atctttatct tatttggaat tgaacgcatg cgtttaattc ctgggacaac ggcatcgaat
     3301 tcattgaagc tcgacttcat gccttacacg catagtttgc ttggaacgtt tttgtgggcc
     3361 gtgggtgcgt tcgttttgtt tcgcttcgct ttgctcagaa aatcaggtca tgagaatcac
     3421 accagaaacc tgatttcgtt tgctgtggcc attgcagttc tttcccacta ttttttcgac
     3481 gtacccatgc acctgcgtga tcttcctctt gcaggggatg attcatacaa actgggtctg
     3541 gccctatgga acaatcgcat cgcggccgtg tctcttgaat tctttctgac tctgggaatg
     3601 tttgcagtct acctgacagc aagtaaacca ggccagggtt tcgccggaaa atatggcatg
     3661 cccattttcg gagctgctct tcttgtgatt tgcgtgctgc aatatatcat accaccgccg
     3721 actaccatca tcgagttatc ctctcaggcg cttgcatcgt acattggcat tggccttgtg
     3781 ggagggtggc tggatcagaa gagagtatat tcgtaaacgc taacctgcta tccacgtcgc
     3841 gcaagaaatg agcagcaagc ccagccatac aaatgaccac ggaaatggga acacccagga
     3901 ctgaaggaag gattttgagc gtcccagact caagaccagg atgactccgg ggaagcaagt
     3961 taccaggaga atcgcccaca acaggccgtg gtccctgtag gtttccttta gtagaaagac
     4021 aaaaccaaaa aggaaaatag gaacacccgc gataaaaagt agactggcga tgacagtgcg
     4081 cattttgcgt tcgaatgttt cgcaaaacct gtattaggtg agaggatttt tctcgaacca
     4141 ctcgacagtg cttcgcagcg tttgatccaa tggccgtggt ttgtaactca gctcttttcc
     4201 cgctctggca aatgagtaaa acccgtaccg gtttgtaatg ctcttcatag cctgaacagt
     4261 ggccagaggt tctgactttg tgattcctgc tgcaaactca aacatggcag ccattcctat
     4321 ggcgagcggc cttggcagtt ttatgccggg cggttttttg ccagaaatct ttcccaggat
     4381 ttcaaagaag tctacgagct ttacattttc acccgagagg atgtatcttt ctccggagcg
     4441 ccctttttgc atggcaagaa tgtgtccgga agcaacatct tccacgctca ctgcactgaa
     4501 tccagtttcc agataggccg gcacttttcc ctgtaagaaa tccaggacta ctttattgga
     4561 tggcgttggt ttgagatcca gcgcaccgag caccagcgcg ggattgactg cgaccagatc
     4621 aattccaaat tcgcgcgcca gagagtgtgc aagcttctcc gattccaact tcgcgcgcgt
     4681 gtattcatcc ggatccgaat ctgcagtcca gttcgattca tccagagggc gagttgggtc
     4741 gtctgtcatt ccaactgcag ccacagaact ggtatagaca actcgcttca caccgccggc
     4801 cactttgact gcttctagaa tgttgcgcgt gccctgcatg gctgtttcga gaatctgtcc
     4861 aggattcttt ctggagagag aatacacagc cgcagtgtga taaacgaaag gcattccagt
     4921 gactgcagca acgagtgagg ggcgatcgta tacgtctccg tagacgattt ccagattctt
     4981 atgatccgga aggcaggcgg tgttgctgga tttgcgaatg agagcccgca ctttttctcc
     5041 ctgatccagg agttgtttca ctagattgct tccgatatgg cccgatgcgc cggtgacgag
     5101 tgctggcatg tgtcatttag acatgtgcgg atcaccgacg caaggaggaa atgcaatcaa
     5161 cgcatttccg caagacctgc ttccagagtc ttaactggag agtatcctag ttctcgtttg
     5221 attttcgcaa tgttgtacct tctgggttgc gccacgaatg cgagttcgaa tcgggagagt
     5281 ggcggagatt tttgaatccc gaataatttc catactccct caatggcgcc tgcggcgagg
     5341 gctgcgatct tcccgttcat attctttttg ggaattggaa ttgccgccgc cgcaaaaaga
     5401 gcagagaaga aatcaaaaac tttcattggt ctgtcatccg tgacgtagta gatagacttg
     5461 tcgctgcctg ccccgagcga gagcacaatg gcctgcacta cattgtctac gtgtaccatt
     5521 tcaatgatgg tttgcccatg gtcgatccac atgaattcgc ctgacgttac tttctgagcc
     5581 atggaatcca gcgctttgac tcccttgccc cagatgaaag tagggcgaag gatgattctt
     5641 acgggcccgg tcggctgagc gcagagtgcg atttctgttt ctttcttggc tctaccgtaa
     5701 acagaactcg gaacagacgg gtacggtgct gtttcatcaa tatcaagaag cggcaacttg
     5761 tcctggatca ctgactcaga gctgatctga atgaagcggc ccacttttct gcgcgaggca
     5821 gcctggtaca ggtcgatggt cgcctgcact gtatcgcgat aaaagtcttc ccattttccc
     5881 cagaactcaa cgggagccgc gcaatggacc accgcatcaa ttccgtcaag agccgtttcc
     5941 cattcggaaa tatttgaaaa cgttccgtga accggctgtg ctcccattgc cttaattttt
     6001 tctgcctggg agcttcctcg ggccagtgcc accacttcat gtttgtctgc tttgagtctg
     6061 cgaatgagcc tttctccaac gaagccgctt ccgcctgtta cgaatacttt catagtccct
     6121 ccatgtcttg cttgacttac tttccctgtt ccataagtaa gtaaatactc acttacatca
     6181 aggaaaagtc gtggcaacta aaaaagcaac taaaaaacgt atgcctgcgg cagcgcgtcg
     6241 tcagctcatt cttgaatccg ccatgcaatt gttcgcgaaa aagggcttcc acggcacaac
     6301 cacagcggat cttgcacgcg ttgcgggcgt atctgaggcg cttatctttc gcttctttcc
     6361 ggacaaggag tcgctctatc gagaagtgca gtttttatgc gtggaggccc gtggtgccgc
     6421 cgattccatg ctggaccagg ccaggcccgg tacagaaaac ctggtgcgcg ccgtgctgat
     6481 tctgatttac gaagtcttct ctggttttgg aggtcgcacg aagaacgaaa cgcttcgcag
     6541 gctgcagact catagcatgc tggaggacgg aaagtttgcg cgcgccttct tcaaaagcca
     6601 tttcaaggtc tattttcctt ttatagaccg atgtctgaaa tcagcgcata ggtctggcga
     6661 ccttgagccg ggcggtatgc cactggagtg cgtcatctgg tttactcatc atttgcccat
     6721 gatggttcgc atcagcatat tgtcgcatcg tgcggactat tttgcgtcaa gcgacgcgga
     6781 gctcctcgag agcatggctt tgttttgcct tcgcgggata ggcctcaaga gctctgcgat
     6841 caaaaagaat cttccgcgca attggacgga gcaatttaat ttattgaaag gataaggtgc
     6901 cccttgcatt ttcgacgcct atgagagcaa cggtacttgg atcgggcagt tttggaacgg
     6961 cgcttgcgag cgtgctggca gacaagaacg cagaggtatg gatctggagt cggtctccag
     7021 atatggcgcg cgccattcat gagcagcacg aaaacactaa gtatttacca ggttttcgac
     7081 tggcagacaa tatcaagtct tcgccggaca tggcggagtg tctcaaggat acagatctca
     7141 ttgtatttgg aatcccctct cagtctctta cgcaggttct caaagaaaac actcatatct
     7201 tgccggaagg agttcccatt gtatcggcag ccaaaggcgt cgaccgtgct acgcttcgcc
     7261 tggtgagtga gattttcgaa gaagaacttc cggggaagtt tcacaaatat cttagctatc
     7321 tttccggtcc cagttttgca aaagagattc tcaaacagat gccaacggtt gtgagcattg
     7381 cctcgcgcaa tgaagaaatc gcaaaacagg tccagcagat gtttgctcac acatggtttc
     7441 gcacttactg gacacccgat gttacgggag tggaagtggg tggtgcaatc aaaaacgtaa
     7501 tcgcaattgc tgcgggtgtt tcagacggtc taggtcttgg gcacaatact cgcgcggcac
     7561 tcatcacaag aggtcttgct gaaatcacaa agcttggtgt gcgaaagggg gcggacccgt
     7621 tgacctttct tggtctggct ggactcgggg atctagtgtt gacttgcact ggagaccttt
     7681 cgcgcaatcg cacagttggc ttcaagcttg gacaaggaat gaagctgcag caaatcctga
     7741 cggagatgaa tcaggtggcg gaaggtgtcg taaccaccga atcggctcat ttgcttgccc
     7801 agaagctcgg aattgagatg gcgattacgg atcaagtata ccaattgctc tatcaggaca
     7861 aggatcccaa acaggtagtg cgagatctca tgacgcgtga tctaaagcgc gaataagggg
     7921 cgattttcat attttttgtt tcctgtgggg gcctcggtcg gcgttattga cctggaatga
     7981 tccagttcga tgaaagtaag attcctccgc tgcccgcagt cgcagcgcga gtgatgcagt
     8041 ttgatcctgg cagccccaac tcttctatcg caacacttga acgcatcatt gcgcctgacc
     8101 agggaatcag cgcggagctt cttcgcgttt ccaattccgc ttactatgga agatccggct
     8161 ccataaaaac tctgcgcgac gcgatcacac tgcttggttt gaaaacggtc aagagcatgg
     8221 taatgctcat ctgcactcgc acattcacgc agaagattcg cggagacctt cttcgcaaat
     8281 attgtattga gtttccgatc gtaactgccc tcctttgcat agacaacgcc cgcgcggcca
     8341 accacaagga tcttcaagaa gagtcttttt tatgcggatt acttcatcgc atcggaatga
     8401 ccatgcttgc tgtggaaaaa aaatcagaat atgctgagct gattgcgagg gctgagaaag
     8461 aaggggcatc tcttgttgac cttgaaaaag acgcattcgg catgactcat gatgacatca
     8521 atgccctggt catcaagaaa tggaatttgc cggaaatcac tgtgcggggc attcttgatt
     8581 gtgaatttcc ggcggaggcc acgaacaaag taagccacca tgcgaagatc actgcgcttt
     8641 cgcgcacaat tgcctctcag ctgttggata ttccaaggcc tccggctgaa atggagcgtg
     8701 caaatgctgt catcaaggcg tatggcctga aggcggatct gttctctcac tacactcctg
     8761 actattttga tctactaaaa gagaatccct tctatcaaag tgcggtggca gtcattggat
     8821 gaagaacaaa agcgacccag ggtcgttctt gtagaggatg atcttgttca gcgcgaaatg
     8881 atcgcgcaga tgctgatctt tgcgggctat gacgtgactt ctgcggcaac cggtgaagaa
     8941 ggacaggaac tcattgaatc agtgaagccg gatgcagcgg tcgtggattt gcacctgcca
     9001 gcagaaagag gcgttaccgg cctcgtgctg ttaaagagta ttaaagagaa tcctgagact
     9061 gcagaactcc cggtcttgat catcaccgcg gacactacag aagaaaccta tgttcttgtt
     9121 ctatcaagcg gtgcttctga tattttgctc aaacctcttc gcatcaccga attgaccata
     9181 cgcatccaga acgttctgga gctcagaaag aatcgtgcgg atttgcgcga agcaaatgcg
     9241 aaacttgagc gcgaaaagaa acgtctcctg cgatattttt cggaagattt tgtaactgcc
     9301 atgctcaacg atgagatttc cgcagagctt ggcgggatca gcgtggatgc aacgatcctg
     9361 tttatggaca tacgcaattc aaccggaatt gccgagcaga taggaccaca gaagttcgca
     9421 gagtttgtaa gtatgatctt cacagatgtg atggatctga tttttgcaaa tcgcggttca
     9481 gtgaacaagc tactgggaga cggaatcctt gccacgtttg gttgtcccgt ccccagcgaa
     9541 aaggatgcgg agaatgcggt gaagactgcc agtcagattc gcgagtattt ccaggtaatc
     9601 aatgaagctc gcgcgcacga agacatcggc ggggacattg gttttggaat tggtgttgcc
     9661 agcggcaaag tattcgcggg caacatagga tcgtttcgca gaatggaata cgccgtgatg
     9721 ggagatcctg tgaacctggc ggcacgtctc caggagttga gcaaaactct gagcgtggat
     9781 attgtcatcg atggaactac aaaatctaag attccggcca gctatgctgt gagagattcc
     9841 ggcgtcaaaa acgtcagagg acgccacggc gaagtagaag catggattct gagctgagag
     9901 cgctcaagga accacaagcg tattagagga aagtccggta tccagagaca ataggtcacg
     9961 cagaatcaaa gatcgcactc ctaccagtgt ccccgcagtc agagccagtg gcacggcgca
    10021 gactctgttt gaataaccgg cgctgactgc tgtttgtcct ggtttcactt caattacata
    10081 gctgatggcc tggttcggaa gaactgccag aggcctcgag cagtcgatcc ctgaaagcga
    10141 attggcagtt ctcactccag tgtccgtggt tccctggaac aacctgtagc ctgcaaatcc
    10201 aatctcagtg ttttgagctg ccatagtgat gatgtggccc gttccttctc gcgcaatgga
    10261 agtgagtatg ggcggattta cattctgcgt ttccgccgaa tagttggtac aggccccgag
    10321 gatcagggca aagcatggga ggattcgcct cattgcagtc tgagcagtcc taccagacac
    10381 aggcagagcc gcacgtactt ttcgatttta ccggaatagg gtcttctggg tcgcagaatc
    10441 cgccacgatt gcgctttgcc cagttcttcc aggtttttcg aaggatggac gagcgttttt
    10501 ttttcgcaaa ttcgaagaag aggaaggtct gcgcttgagt cagaatagcc agtcgaattt
    10561 gcaatcggcc gcgcatcaaa aagagacgga ttcttactca gtctctcata gtccgtgcga
    10621 atggattcga tgagagattc accgcagatt tccggcatgc gaaggagctt ttcaattccg
    10681 cggttgttct tgctgtggat tctcgggaaa agaggcatgc gctcttcgag caccacttcc
    10741 gtggccacta cttcgtcaaa tcccgccgcc t
//
LOCUS       DAOQJL010000060        10549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3256_length_10549_cov_4.692300, whole genome shotgun sequence.
ACCESSION   DAOQJL010000060 DAOQJL010000000
VERSION     DAOQJL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_10549_cov_4.692300"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PLG78_04275"
     CDS             <1..1347
                     /locus_tag="PLG78_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE22353.1"
                     /translation="VYKRAGDLILSGTRALTQLRMECSAVSSESALARISALAEESMD
                     QAPRVLRFTDRASRFFIAFALFAAVFTFSYWYFFKHDVHSAILNMITLLIAACPCALS
                     LAVPTVFTVSVQKSFDLGCLLKNGSTLELLSRAKAIAFDKTGTLTRGEPEIVQVSVVD
                     DIFSEEYVCALASELQRRADVRHPIARAFYSLQRLTGSSANLPDLGRVEYKPGLGIVS
                     SSPEASYALGSRRLMESVGVECQPAGAPGSISIYLARLGGRVPELVSVFVLADAIRPE
                     ASDVLRRLSRNSSLFLLTGDTVENAAQVSKQLGITEIYAEKTPEQKREIIQARQASGI
                     TIMVGDGINDSVALAQAHAGVSFANASEISLHSADVLLLRNDLRQFLSLVQLSRRARS
                     IVLQNLGLSFLYNMFLIPMACAGWIIPLAGALFMSLSSLTVVLNSLRLLSFRKEIG"
     gene            1388..2299
                     /locus_tag="PLG78_04280"
     CDS             1388..2299
                     /locus_tag="PLG78_04280"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNE22354.1"
                     /translation="MRKVALCLVLVSAFISSVDAENLVAVPQLQTRVTDLTGTLSASE
                     NAELSKILADYEKGKGSQIAVLILPSTRPETIEQYSIRVAEQWKIGRKGVDDGVLLIV
                     AKEDRKLRIEVGYGLEGVLPDAKAKDIIDEVIVPYFKNGDFYGGVKIGVETIIATIKN
                     EPLPPPKFKPDAKFAKQAATAIGMGIVVSLIVGAILSALPVIRHLIALTIYLVILYFV
                     GLLFGSSMASLVHAGITGLFLLGVRYLGGTSAGKAWNSDSSSSWSSSSSSSSSSWSSS
                     GSSSDSDSSSTSGGGGSFGGGGASGSW"
     gene            complement(2326..2706)
                     /locus_tag="PLG78_04285"
     CDS             complement(2326..2706)
                     /locus_tag="PLG78_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22355.1"
                     /translation="MIRTLFLFWLGLATLIEFGLALGGLFAPDFLRAQFHIGTTEDTR
                     FLTYVVAWLILMIAIACALAWRWSFLGKSAGYVLTNWLGLFWIGIGIGIYFQSNGVRT
                     DNLALDSAKGAILILLNYLNDRFG"
     gene            2755..4992
                     /locus_tag="PLG78_04290"
     CDS             2755..4992
                     /locus_tag="PLG78_04290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE22356.1"
                     /translation="MHSGRCWLISTVQANIQITGMTCATCALRIEKGLSKMPGVENAT
                     VNFATGSAHVTYADHQVARDAMLARIHDLGYEGVIPNEESEITQIKQKEVRKLRRRLI
                     LSAVLSAPLLWTMGSHIPGLHIFVPQILMNPWVQLLLASPVQFIIGFAFYRGAFRALS
                     NATANMDVLVALGTSAAFFYSLFLALSGHSHQGLYFETSAVLITLIVFGKFLESVATG
                     RTSGAIEKLMKLQPDRASVVRDGTEIQISLDQVVKGDRINIRPGERIPVDGRVIDGDS
                     HVDESMFTGESVPVHKRKGDSLLAGTMNTNGALQMEADKVGRDTALAAVVRAVEEAQG
                     SRAPIQRVADQISSVFVPVVVLVALAAGVLSYLIFVPGEFASALERAIAVLVIACPCA
                     LGLATPASIMAGTGRAAEAGVLFRGGEHLEAAGALDLIILDKTGTVTMGQPALTEVTG
                     EIPESEILRIAASAETSSEHPIAQAIVRAASERSIVTSPVSGFKATPGQGIAAELEGK
                     EILLGTEAWLAQSNISPGDGTASAILESQGKTVVYLAVDGRYAGLLAISDQLKPSSAA
                     AIQRLHELGLETYMVTGDNERAARWIASQCGIDRVAARVLPEGKARIVSEMKQAGRKV
                     GMVGDGINDAPALATADTGFAIGTGTDVAMEAADVSLMSGDLGGVADAIEASRRTMRN
                     IRQNLFWALAYNSLGIPIAAAGLLAPWVAGAAMAMSSVSVVTNALRLQRMKIGNRTVR
                     REEVS"
     gene            4989..5525
                     /locus_tag="PLG78_04295"
     CDS             4989..5525
                     /locus_tag="PLG78_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22357.1"
                     /translation="MNRLGIFVFVLILGCEHFEGELLAPKEWIEIPGLEGRWDVPHTT
                     DEPISIVKSTQAKEYRIVPRKGDVPGVVRFASIDGRIIGEYRDEKEGKPVRQGYIFRM
                     EKSGDCFLVSVGQEITEQMVNKYRLIARAEKSLKTSLPTYVLNGTAAQNRSALDALLK
                     DPHFFPERPGLKICRVAN"
     gene            complement(5522..6430)
                     /locus_tag="PLG78_04300"
     CDS             complement(5522..6430)
                     /locus_tag="PLG78_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE22358.1"
                     /translation="MKIKTLILAGGTALITFAGASWYFSSVIILSQKHSVEQDRINLK
                     LNSPADFGLPQPESVVIPTRSGHISAWLFRNPSGKKCAAILHHGHTGTRWGGLKYARL
                     FFERGCAALSVDGRYHGESSGDFCTYGYYDRYDMVDVAAWLKKELVLRDSEVGMMGES
                     LGGAVSLLTAAETTLAFVAADSPFSNASAIFHEQAEKQYGPAIRVLIPVAFFFAEKRA
                     NFKIKDVSPMDAAPKITAPVFLVHSLQDDYTSPYHSELIYKAVSHDRKVLHLTDRGAK
                     HGKSIDMNYAEYKKWMDEFLSRYTHF"
     gene            6574..8916
                     /locus_tag="PLG78_04305"
     CDS             6574..8916
                     /locus_tag="PLG78_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE22359.1"
                     /translation="MQFTNHTVGRIAPALLTLIISLYFFVRGRGQKASFWLGLYFALL
                     SVFNIGYIFGYSVDHPRGGYAWYLACAISFAAAARIQFAYSFPSEFRPREKRAALILA
                     FAASGAALVDYGLRVGSDFGFNLKHHLYGSTYSSLWVPMVSFVCFFWSIVVSVRRALE
                     LLRSGRKKSGSLVFAWRNNPEFRAVCYLAGLTTMELLVNGAYLLEYGRAVRTQFLPQF
                     MNVSLLVIFGGYVLLYSTAPSTRTGFLSKLVGVCLVSVLPMIGALGSAFQNMDLDRFL
                     GERMLVGRTALQYLRQGNPVPSSVAFIVTDSDSPVYAITPGAADAARSTDVFDGRFYF
                     AQSHSERYFSAIISDEAGHRTRAGFRYEDYRARLDESSRLIVYALVMAALIIALLFPF
                     LFRASLLMPLRLLLNDLKQIKESKVDPGLDEIGALRASFGRMLDLIRETSKRIPDFAP
                     HLNQMEDLAATEPGRIEIGGRTLIYRSRAMRRLIEQVARAREYRFPVLITGETGTGKE
                     LVARMVHSDDPVPFVAVNCAALPETLWESEVFGHKKGAFTDAKSDRRGRIVEAGDGVL
                     FFDEIGEMPLAMQAKMLRLLQEGQFSPVGSDLTLTAGCRFIFATNRNLADLVKEKKFR
                     EDLLYRIRVFHLEVPPLRERPEDIGDLLRFFMERFARDHKRTVPSLEPAALHALVQYR
                     WPGNIREVENAVIRAMAAGSDVLGSELFPEVGGARHASGASSGVAVAIDLDSEIRRYS
                     RSLIERALEQAKGNKTQAAELLGIKRTTLRYRMRDIGLED"
     gene            9105..9959
                     /locus_tag="PLG78_04310"
     CDS             9105..9959
                     /locus_tag="PLG78_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22360.1"
                     /translation="MKKYLTSMIALIALSGSIYAEKVIYVHGMSLTESNAHCQDTKDC
                     AYWESHVGGDYVFVGYDGRQNPFGGTDVSGSVRLLQLLNKYCRKDQGQSCRLIDDSLG
                     GFTGAGTVSMYNKTGLYNILYVTQIVSAEGGSEIANLGDTAIDILKVVFNLNGDGAQA
                     LIDSHSAIQALVTTSARALFDHNRSNGTVFYHVNANKSFFLADPFLPGTDDSLVAFHS
                     SCGYRTTGAFSKCMGEKIRSCALCFWETPKLITPFDNHKMHPLVPLTGIDIPHAQGHH
                     DVRYHGAL"
     gene            10009..>10549
                     /locus_tag="PLG78_04315"
     CDS             10009..>10549
                     /locus_tag="PLG78_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22361.1"
                     /translation="MNRKYFTIFIIVAVFLTVGLALLLIPDNKQSRHGSDSARSLSDP
                     SRDMGADPAAGIDPEQLIADYKEWAKYPPDSRPLLESHVDVIDFRKIPNTVQRMPTRD
                     GDKLRESGFSCLLQPEKHTATEGENMRIFLSCTHTGRPERESIRIKEIKLEAVAGSRR
                     FMPIPPTVSDAGDKGDESAG"
BASE COUNT         2421 a         2516 c         2872 g         2740 t
ORIGIN      
        1 gtctataaac gggccgggga tctcatcctc tctggaacgc gggcgctcac gcagcttcga
       61 atggagtgtt ccgccgtaag ttcagagagt gctctggcca gaatcagcgc acttgcagaa
      121 gagagcatgg atcaggcgcc cagggttctg cgattcacag atcgcgcgag caggttcttc
      181 atcgcctttg cgttgtttgc tgccgtattt actttctctt actggtattt tttcaagcac
      241 gacgttcatt ctgcgattct caacatgatc actctcttga tcgcggcctg tccatgcgcg
      301 ctcagtcttg ctgtgccaac tgtgttcacc gtttctgttc agaagtcttt tgatctgggt
      361 tgtttgctaa aaaacggcag cacactggaa cttttgagtc gtgcgaaggc catcgctttt
      421 gacaagacgg ggactctgac gcgcggagaa ccagagatcg ttcaggtgtc tgtcgtagat
      481 gatatttttt ctgaagagta tgtttgcgcg ctcgcgtctg agctgcaaag acgcgcggat
      541 gttcgtcatc caattgccag agcattctat tctctgcaaa gattgaccgg atcatctgcg
      601 aatttgcccg atctgggtcg cgtcgaatac aagccaggtc ttgggattgt ttcttcatca
      661 cctgaggcga gttatgcgct cggcagccgc agattgatgg aaagcgtcgg cgtagagtgt
      721 cagccagcag gagcgccagg tagtatttcc atttatctcg cacgtttggg cggtcgtgtg
      781 cctgagctcg tcagtgtttt tgttctggca gatgcgatca gaccggaggc aagtgatgtg
      841 cttcgtcgtc tatcccgcaa ttcatctttg tttctgttga ccggggacac tgtggagaat
      901 gccgcgcaag tttcaaaaca gctgggcatt actgagatct acgccgaaaa aacgccggaa
      961 caaaaacgag agatcatcca ggcaagacag gcatccggaa tcacaatcat ggtgggggac
     1021 ggaatcaacg actccgttgc cctcgcgcag gcacatgccg gtgtttcgtt tgcgaatgct
     1081 tctgaaattt cactccattc tgcggatgtg ctgctactgc gaaatgatct gcggcagttt
     1141 ctgagtctgg ttcaactatc caggcgtgct cgtagcattg tgcttcagaa ccttggtctg
     1201 agtttcctct acaatatgtt cctgattcct atggcatgtg ctggatggat cattcctctg
     1261 gctggagcgc ttttcatgtc tttgagttct ttgactgtgg tgctcaattc actgcggctc
     1321 ttgagttttc gaaaagaaat cggctaaata gtttgtcgaa tgttcgctat cggattttgt
     1381 tagccccatg cgcaaggtgg ccctttgtct tgttctcgtt tctgcattca tttccagcgt
     1441 tgatgcggag aatctcgtcg ctgttccgca gttgcagacg cgagtcacgg acctgaccgg
     1501 cactctgtcc gcttctgaaa acgcagagct ttctaagata cttgctgatt atgagaaagg
     1561 gaaaggtagc cagattgcag ttctcattct accatctacg agaccggaga caattgagca
     1621 atattcgatt cgcgtagcgg agcagtggaa gatcgggcgc aaaggagtag acgacggcgt
     1681 cttgcttata gttgcgaagg aggaccgcaa gcttcgaatt gaagtaggct acggtctcga
     1741 aggcgttctc ccggatgcga aggcaaagga catcatagat gaagtgattg ttccctattt
     1801 taagaacgga gatttctacg gcggcgtaaa gatcggagtg gagactatca tcgccaccat
     1861 taaaaacgaa ccattgcctc cgcctaaatt taagccggat gcaaaatttg caaagcaggc
     1921 ggccactgcg attggaatgg gaatcgttgt gagtttgatt gttggagcga ttctctctgc
     1981 tcttcccgtt attcgccatt tgatcgcgct gacgatctat ctagtgattc tatacttcgt
     2041 aggacttctg tttggttctt caatggcgag tttagttcat gcgggtatca cgggactgtt
     2101 ccttctgggc gtgagatatt tggggggaac atccgcaggc aaagcctgga attcggactc
     2161 ctcctctagc tggtcttcct ccagttcaag ttcatccagt tcctggagtt caagcggaag
     2221 cagttctgat tctgattcca gttcgaccag tggaggaggc ggtagctttg gcggaggcgg
     2281 tgccagcggc agctggtagt gctgccttct gtcacagatt cgcgatcaac caaatcgatc
     2341 gtttagataa tttagtaata tcagaatggc gcctttcgca ctgtccagcg ccagattgtc
     2401 tgtgcgaacg ccatttgatt gaaagtagat tccaattcca atcccaatcc agaatagccc
     2461 cagccagttt gtgagcacgt aacctgcact ctttccaagg aaagaccatc tccacgctag
     2521 agcacaggcg atggcaatca tgaggatcag ccaggcaacc acgtaagtca aaaagcgtgt
     2581 atcctccgtg gttccaatgt ggaattgagc tcgaagaaag tcaggcgcga aaagaccacc
     2641 cagcgccagc ccaaattcga tgagagtcgc cagtccaagc cagaagagga acagtgttcg
     2701 aatcatggat catgatggtg gccgaaagcc aggattgtca aacaaagaag tagtatgcat
     2761 tcaggtcggt gctggcttat ttcaactgtg caggcaaata tacagatcac tggaatgacg
     2821 tgcgccacgt gcgcacttag aattgaaaag ggtttgagca agatgccggg agtggagaat
     2881 gccacggtaa attttgcgac aggatccgcg cacgtgacgt atgcggatca tcaagtggcc
     2941 agggatgcaa tgctcgctcg aatccatgat ctgggttacg aaggggtgat cccgaacgaa
     3001 gaaagcgaaa tcactcagat caaacaaaaa gaagttcgca aacttcgtcg ccgtcttatc
     3061 ctttctgctg ttttgtccgc acctcttctc tggacgatgg gatcgcatat acccggtctc
     3121 cacattttcg tcccgcaaat cttgatgaat ccctgggtgc agttgctcct tgcttcacca
     3181 gttcaattca tcatcggttt tgctttttac cgtggcgcat ttcgcgcgct ttccaatgca
     3241 acagccaaca tggatgtgct ggtcgctctg ggcacttcgg cagcattttt ctacagtttg
     3301 tttctggctc tatccggaca ttcgcatcag ggtctgtatt ttgaaaccag tgcagtgctc
     3361 attactctga ttgtgtttgg aaagtttctg gaatctgttg caaccggaag aacgtcaggc
     3421 gccattgaga agttgatgaa gctgcaaccc gatcgcgctt ctgtcgtgag agatggaact
     3481 gaaattcaga tttctctgga tcaggtggtg aaaggggatc gtatcaatat tcgtccgggg
     3541 gaacgcattc cagtggatgg ccgcgtcata gacggtgatt ctcacgtgga cgaatccatg
     3601 tttacaggtg agagtgtccc tgttcacaaa cgcaaaggtg attctcttct ggccgggact
     3661 atgaatacaa atggcgcttt gcagatggaa gctgacaaag tgggtcgcga tactgcactg
     3721 gctgcggttg tgcgagctgt agaagaagcg cagggtagtc gtgctcccat ccagcgtgtg
     3781 gcagatcaga tatcttctgt ctttgttccg gtggtggttc tcgttgcgct tgctgctggt
     3841 gtcttatcct acttgatatt cgtgcctggc gaatttgcct ctgctctgga gcgagccatt
     3901 gcagttctgg tgattgcctg cccctgtgct ctgggtcttg caacgcctgc ttccattatg
     3961 gctggaacag gtagggcggc agaagctggc gttttgtttc gcggtggaga acatctggaa
     4021 gctgcggggg ctctcgattt gatcatcctg gacaagactg gaactgtgac gatgggtcag
     4081 cctgcgctta cggaagtgac cggcgaaatc ccagagtcag agatactcag aatcgcggcc
     4141 tctgcagaga cgtcatcgga gcatccgatc gcgcaagcga ttgtgcgagc agcgtctgaa
     4201 cgctcgatcg ttacgagtcc agtgtccgga ttcaaagcta caccaggcca gggaattgcc
     4261 gcagaactcg aaggaaaaga gatcttactt gggacggaag cgtggcttgc tcagagtaat
     4321 atttcacctg gagatggaac agcaagtgcg atcctcgaaa gccagggaaa gaccgtggta
     4381 tacctcgcgg tagatggacg ttatgcggga ttgcttgcga tttcagatca actcaagcca
     4441 tcatctgcgg ctgcaattca gagattgcac gagcttggtc tggaaacata catggtaacg
     4501 ggcgacaatg aaagagcagc acgctggatt gcttctcaat gcggaataga tcgagtggcg
     4561 gctcgtgtgc ttccggaagg aaaggcgcgc atcgttagcg aaatgaaaca agcaggaaga
     4621 aaagtaggaa tggtggggga cggcatcaat gacgcgccag cgcttgcaac ggcagatacc
     4681 ggttttgcga ttggcactgg aacggatgtg gccatggaag cagcggatgt gagtctgatg
     4741 agcggtgatc tgggtggagt ggcggatgca atcgaagcga gccgcagaac catgcgcaat
     4801 atacggcaga acttgttctg ggcactcgcc tataattcgc tcgggattcc gattgcggct
     4861 gcgggtttgc tcgctccctg ggttgccggc gctgcgatgg ccatgagttc cgtctccgtt
     4921 gtgacaaatg cacttcggct gcaaaggatg aagatcggaa acagaacagt ccgtcgcgaa
     4981 gaggtttcat gaacaggctc ggcatattcg ttttcgtttt gattcttggc tgcgaacatt
     5041 ttgaaggaga gttattggct ccaaaggagt ggattgaaat cccgggcctc gaaggtcgct
     5101 gggatgtccc tcatactaca gacgaaccca tttccatcgt gaagagcact caggcgaaag
     5161 agtatcgaat tgtgcctcgt aagggcgatg tgcctggggt agttcgtttt gcatccattg
     5221 atggccggat cattggagag tatcgggatg aaaaagaagg caagccagtc cgccagggtt
     5281 atatctttcg catggagaag tcaggcgatt gctttctggt ttctgttggt caggaaatca
     5341 cagaacaaat ggttaataaa tatagactaa ttgctcgtgc cgaaaagtcg ctcaagacca
     5401 gccttccaac gtatgtgctt aatgggacgg cggctcagaa tcgtagtgcc ctggatgcac
     5461 tcttgaaaga tcctcatttt tttccggaac gcccgggctt gaagatctgt cgcgtcgcaa
     5521 actagaagtg cgtgtagcgc gagagaaact cgtccatcca tttcttatat tcagcatagt
     5581 tcatatcaat ggatttgccg tgttttgcgc cgcggtctgt taagtgaaga accttgcgat
     5641 catgggagac tgccttgtag atcaattctg aatggtaggg acttgtgtaa tcgtcctgaa
     5701 gtgaatgaac caggaaaacg ggtgcagtaa tttttggggc agcatccatc ggggatacat
     5761 ctttgatctt gaagtttgcc cgcttctctg cgaagaaaaa tgcaaccgga atgagtactc
     5821 gaattgccgg tccatattgt ttctccgcct gttcgtgaaa gatcgcactc gcgttgctaa
     5881 acggagaatc ggctgcgaca aatgcaagag tggtctctgc tgccgtcagc aggctgaccg
     5941 ctccgcccag ggattcaccc atcattccga cttccgaatc tcgtagaacc agttccttct
     6001 tcaaccacgc ggctacgtct accatgtcat agcgatcgta gtatccgtac gtgcaaaaat
     6061 cgccactgga ttctccatgg tatctgccat ccacggacag agcagcacaa cccctctcaa
     6121 aaaatagtct agcatacttc aatcctcccc atcgcgtacc tgtatgtccg tggtgaagga
     6181 tagctgcgca ttttttcccg gagggattgc gaaacaacca tgcagagata tgaccggatc
     6241 tagtaggaat gacaacagac tccggttggg ggagtccaaa atctgccgga gaattgagtt
     6301 tcagattgat gcgatcttgc tcaacggagt gtttttgcga aagaatgatc acggatgaaa
     6361 aataccagct cgcacctgca aacgtgatca gagcggtacc cccggcgagt atcagggttt
     6421 tgattttcat tttcgcaaac tctgcgaccg gacgtttgcg gcaatcccaa attgaacctg
     6481 tgcctgtggt cgcataacgg ttttcggtca caaaatttgc catgaattga aatagaattg
     6541 cgaggagcgt atcgcgcgag cgaatgcgcc aggatgcagt ttaccaatca cactgtcggc
     6601 agaattgcac cggccctcct cacgttgatc atttctttgt acttcttcgt gcgagggaga
     6661 ggtcagaaag catccttctg gctggggttg tattttgcac ttcttagcgt gtttaacata
     6721 ggttatattt tcggatactc cgtggatcac ccgcgtggag gctatgcctg gtatcttgcc
     6781 tgtgccattt catttgccgc cgctgcgcgc atccaattcg catacagctt tccgtccgaa
     6841 tttagaccgc gcgaaaagcg cgcagctctt atcctcgcct tcgcggcatc aggggcagca
     6901 ctcgttgact acgggctgag agttgggtcg gactttggtt tcaatctgaa gcatcacctg
     6961 tacggatcca cgtattcctc actctgggtg cccatggtca gttttgtttg tttcttctgg
     7021 agcattgtag tatccgtgcg tcgcgcactg gagctgcttc gatccggtcg caagaaatcc
     7081 ggatccctgg ttttcgcatg gaggaacaat cctgagtttc gcgcagtctg ctatctggcg
     7141 gggctcacaa ccatggaatt gctcgtgaat ggcgcctatc tgttggaata cggacgagct
     7201 gttcgcaccc agttcctgcc acagtttatg aacgtttcgc ttctcgtgat ttttggaggt
     7261 tacgtcctac tctattcgac tgcaccatct acgcgaactg gctttctttc aaaactggtt
     7321 ggcgtttgtc tggtttctgt gcttccgatg attggcgcac ttggatctgc ttttcagaat
     7381 atggatctgg atcgatttct aggcgagcga atgctcgtcg gtcgaacggc acttcagtat
     7441 ctgcgccagg gaaatccagt accttcttct gtggctttta ttgtaacaga ttctgattct
     7501 ccagtctatg cgatcactcc aggtgcggcg gatgcagctc ggtccacgga tgtattcgat
     7561 ggacgtttct atttcgcaca gtcgcattct gaacgatatt tttcagcaat catttccgat
     7621 gaggcaggtc acaggacgcg ggccggtttt agatatgaag attaccgagc gagactcgat
     7681 gagtcctcgc gattgattgt gtacgccctg gtgatggcag cactcatcat tgcgctgttg
     7741 tttccgtttc tgtttcgcgc gagtttgctc atgccgcttc gcctgcttct aaacgatctt
     7801 aaacaaatta aagaatcaaa agtggatcca ggcctggatg aaatcggcgc actgcgtgct
     7861 tcgttcggca ggatgttgga tttaattcgt gaaaccagta agcggattcc ggatttcgcg
     7921 ccgcacctga atcaaatgga agacctggcc gccacagagc cgggtcgcat tgaaattgga
     7981 gggcgcacgc tcatctatcg atcacgtgcg atgcgaaggt tgatcgagca ggtcgcgcgg
     8041 gcccgggagt atcgctttcc tgttttgatc acgggtgaaa caggcactgg caaggaactc
     8101 gtcgcgcgca tggttcattc agacgatccg gtgccgtttg tagcagtgaa ttgtgcggca
     8161 ttgccggaga cactctggga atcggaggtg ttcggacaca agaagggagc attcacagat
     8221 gcaaagtccg atcgtcgcgg acgaattgtg gaagcaggag acggggtttt attttttgat
     8281 gagattggag agatgcctct ggccatgcag gcgaagatgc ttcgtcttct tcaggaaggt
     8341 cagttttctc ctgttggttc agaccttaca ctcacagccg gctgccggtt cattttcgca
     8401 accaacagaa atctagcaga tctagtcaaa gagaagaagt ttcgggaaga tctgctgtat
     8461 cgcattcgcg tgtttcacct cgaagttcct ccgttgagag agcgccctga agacataggt
     8521 gatttgttgc gtttctttat ggaacgattc gcgcgagatc acaaacggac tgttccctct
     8581 ctggagccag ctgctttgca tgctctggtt caatacaggt ggccgggcaa cattcgcgag
     8641 gttgaaaatg cggtgattcg tgccatggcc gcggggagtg atgtccttgg ttccgagtta
     8701 tttccagaag tggggggagc ccgtcatgcg agtggtgcgt cgagtggggt ggccgtggcc
     8761 attgatctgg attccgaaat tcgaagatac tcacgttctt tgatcgaaag ggctcttgag
     8821 caggcgaagg ggaataagac gcaggcagcg gagctcctcg gaatcaagcg cacaacactg
     8881 cgctatcgca tgcgcgacat tggcctcgag gattgatttc tctttcgatc caatccggcg
     8941 gtggcaaatt tttgccggtg acaaaattct gccagtaacg gtttcttaaa ttagccacaa
     9001 aaccagtgtt tcaagacaaa tgacctcact taactccctg gtacgcgacc tgcaataggt
     9061 tccccgtgag cctagcggct caacaaacaa aacaggggga gagaatgaaa aagtacctca
     9121 cgagcatgat tgctctgatt gctttgtcag gttccattta cgcggagaaa gtgatctacg
     9181 ttcatggaat gagcctgact gagtcaaacg cacattgcca ggacacaaag gattgcgcat
     9241 actgggagtc gcacgtcggc ggagattacg tcttcgtagg atatgatgga cgtcagaacc
     9301 cgttcggtgg aaccgatgtg agcggaagcg tacgtttgct ccagcttctc aacaaatatt
     9361 gccgcaagga tcaaggccag agctgcaggt tgatcgatga ttcacttggt ggtttcaccg
     9421 gagccggcac tgtgtccatg tacaacaaga caggattgta caacatactg tatgtgactc
     9481 aaattgtttc tgcggaaggc ggttccgaaa tcgccaatct cggagacact gcgattgata
     9541 tactcaaagt agttttcaat ctgaatggtg atggagcgca agctctgatt gactctcaca
     9601 gtgcaattca ggctctcgta acaacgagtg ctcgtgcatt gttcgatcac aatcgttcca
     9661 acggcactgt cttctaccat gtaaacgcaa acaaatcgtt cttccttgca gatccatttc
     9721 tgcctggcac agacgattct ctcgttgcgt tccacagttc atgcgggtac aggacaaccg
     9781 gtgcgttcag caagtgtatg ggagaaaaga ttcgctcctg cgcactttgt ttctgggaaa
     9841 ctccgaaact catcacgccg tttgacaatc acaagatgca tccgctggtt ccgctcactg
     9901 gaattgacat tcctcatgcg cagggccatc acgacgtccg ttatcacggc gcgctttgat
     9961 ttctctttga gccggttcgc cggctcaatt tttttgtggg ggatggttat gaatcgtaaa
    10021 tactttacaa tctttattat tgtcgcagtt tttttgaccg ttggtcttgc gcttcttctg
    10081 attccggaca acaagcagag cagacacggc agtgattctg cccgatctct ttccgatcca
    10141 agtcgggaca tgggtgcgga tccagccgct ggaattgatc ctgaacagtt gattgcagac
    10201 tacaaggagt gggcaaaata cccgcctgat tcgcggcctc tactggaatc gcacgtggac
    10261 gtcattgatt tcagaaaaat cccgaacaca gtccagcgca tgcccaccag agatggcgat
    10321 aagcttcgcg agtccggctt ttcgtgtctg ctgcaaccgg agaagcacac ggccacagag
    10381 ggagagaaca tgcgcatctt tctgtcctgc acgcatacag gcaggcctga gcgcgaaagt
    10441 atccgtatta aggaaatcaa gcttgaggcg gttgcaggtt cgcgtcgctt catgccgatt
    10501 ccgccaacag tgagtgatgc gggtgataaa ggggatgagt ccgcggggg
//
LOCUS       DAOQJL010000061        10537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3262_length_10537_cov_4.165903, whole genome shotgun sequence.
ACCESSION   DAOQJL010000061 DAOQJL010000000
VERSION     DAOQJL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_10537_cov_4.165903"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLG78_04320"
     CDS             complement(<1..212)
                     /locus_tag="PLG78_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE22362.1"
                     /translation="MKGKRILVIDDDESTRALVSHALTKAGAVVVSAADADGAMKAAS
                     HDRVDLILLDLKMPDFDGMTLLRTLG"
     gene            365..1567
                     /gene="creD"
                     /locus_tag="PLG78_04325"
     CDS             365..1567
                     /gene="creD"
                     /locus_tag="PLG78_04325"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNE22363.1"
                     /translation="MIRLTIKMALIGALSTMLLIPTAYICSLTKERKDRRDEVARSTG
                     NLWGSHHEIGTILEAGKESILPQSVDVTGSVLAETRNRGIYSLPFYSADLTMQVQFFL
                     ERESQASVIRLYGVKASEADFLTSKLDDLEIRFKGDDEPRQVAAHAGTLSRGTHKLTI
                     HFKMNGTQRLHFVPAANTLTVDLKSNWPHPNFSGSILPAERTIDANGFHAKWIVKPQN
                     ASAFLAELKNNEYRETLTEGEEATSFGVDFFVPVDIYAQIERASKYAMLFISLTFLTF
                     FVFEAVAGLQIHPVQYLFVGLALSLFFLLLLSLAEHIGFARAYGLASVADLGLITAYS
                     LKILKSRTRAGILLGVLAGLYSFLYVVLQLEMYSLLAGSIGLFLILAIVMYATRNIDW
                     YAKEPAKA"
     gene            1683..3359
                     /gene="ilvD"
                     /locus_tag="PLG78_04330"
     CDS             1683..3359
                     /gene="ilvD"
                     /locus_tag="PLG78_04330"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE22364.1"
                     /translation="MNLNWNSKQITQGDRRAPNRAMMRAVGFEDSDFDKPVVGVASGH
                     STITPCNAGIRPLEEAACKGISSAGGMPQTFGTITISDGISMGTQGMKLSLVSRDVIA
                     DSIETVCRGQLMDGVLAIGGCDKNLPGAMMAIARLDIPGIFVYGGTILPGKYQGRDLN
                     IVSVFEAVGSFQAGTISREELNEIEKHACPSSGSCGGMYTANTMSSAIEALGMSLPNS
                     SQMSAPSPEKKSSAEESGEALVRMIRTNLRPSQIMTRKAFENAIAVVMATGGSTNAVL
                     HLIAIAHDMGITLTLDDFEAISKKVPHLADMRPSGKYLAVDFHRAGGVPLIMKLLLKA
                     GILHGECMTVTGRTVSENLAEVPDAPPAGQDVIRPFSSPVYKRGHLVILRGNLAPGGA
                     VAKTSGVKSPAITGEARVFDSEEECMDAIMSDQIKPGDILVIRYEGPKGGPGMREMLS
                     PTSALIGKGLGDSVGLITDGRFSGGTYGMVVGHVSPEAAVGGPIALVREGDVITIDAY
                     ENLLQLHMTEAEMEARRKEWVKPESPVKNGVLYKYAKLVGSAEFGAVTDA"
     gene            3411..5420
                     /locus_tag="PLG78_04335"
     CDS             3411..5420
                     /locus_tag="PLG78_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HNE22365.1"
                     /translation="MKTDHLILDESGKQTRDKPWIFRTYAGHTSARASNELYRTNLAA
                     GQTGLSIAFDLATQCGYSSDHPIAKPEVGKVGVPINSLDDFHILFDQIPLEKMNTSMT
                     INATSMWLLSLYIALARERNESPKKLQGTTQNDIVKEYLARGTYIFPPQASLRMIAEM
                     YEYCLKEVPEWNPSNICSYHLQEAGATPTQELAFALANAIGLLDLIRERGTFTHDEFE
                     KCVGRISYFVNAGIRFVEEMCKMRAFTELWEEITRDRYGVKNAKYRMFRYGVQVNSLG
                     LTEAQPENNAWRILIEALGVTMSKRARCRALQLPAWNEALSLPRPWDQQWALRLQQIL
                     AYETDLLEYPDLFDGSKVVESKVEALKEECRAELDKILGMGGVRAAVESGYLKTSLVK
                     SMSERLSKINSGELTVVGINKWKDGIASPLTSGNDGGIFKVDPAKVKETMDNLEATKR
                     KRDPAKVEAALAKLKAAAKSGENMMEPSIECALARVTTGEWSDALRSVFGEYRPATGI
                     DGQKLLLEDERVASVRKRVETFTAKRGHRPRIVVGKPGLDGHSNGAEMIAVTARHSGF
                     DVIYSGIRLTPEEIVQSAVEENADIIGISILSGSHIELAEQIVAKLKEMKAERIPVVF
                     GGIIPKEDFAVLEKTGIRKLFTPSDYKLMDIMDSILNVVEEESAK"
     gene            5444..6325
                     /locus_tag="PLG78_04340"
     CDS             5444..6325
                     /locus_tag="PLG78_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE22366.1"
                     /translation="MSDFENTLPEAALRRHDHIERLKSLPEARKGVFAGFTGTPGAGK
                     STLIGALCLRLIEMDPHIRVAVVAVDPSSEVSGGALLGDRTRVRFPVDEDRLFFRSQA
                     SDRQLGGMSPGTFQVCRLLYHLFDLVIIETVGIGQNEIEVQRLADRVYLVLQPLAGDQ
                     IQFLKAGIMEIPDVFLLNKSDEVEAARKTYHALKASIQFTRPGDGDIQILQTSAVMGT
                     GIDEVCADLMELVRKPRDGESLMREKEERYFSQWVREEYGRSGIKKLLDLGGAAFWIK
                     ECGSYDAATRRLIDKFN"
     gene            6332..7120
                     /locus_tag="PLG78_04345"
     CDS             6332..7120
                     /locus_tag="PLG78_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22367.1"
                     /translation="MQIDFFEEFPSEETLSKAKLMNGEFLVYIASSTISDFHRHRDML
                     SRISSNAKAAYWPLLRRSYWVSPFSFPEELEGLYRDLENSSPMDVLIDLELPLLRPAL
                     FLKNVPHFLENKKLIRKILGLGHRFLAAVYPATGILSESILRALGIWHPQTESCVMYY
                     TSMIQKPAIIRWMQSAIRRRALLGHRTVGLGCLAPGIFGTEPVITTEGLKRDMDFLTE
                     AGVKRAILFRLGGLDSEFASILCGNKKIALPGSEEKQGICADWD"
     gene            7102..8760
                     /locus_tag="PLG78_04350"
     CDS             7102..8760
                     /locus_tag="PLG78_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE22368.1"
                     /translation="MRRLGLILFVFGISALMAQDPAGPAQDEMKTSETARPAASDSKQ
                     ATTTPVSQNNAQATYSKVPTGPDGKPLPLSMEQIVKMVLENNNLVRIQQLEILKSDTD
                     IMKDESKYAPKIGASYEGYEKLDKSSASTIFSGDKVNQDKYTAYMNKLFSSGTYFQVE
                     ASDTRFDSNAGESFALQGTTFAKLAQPPLHTGALTLTLRQELLKNAFGYSQRRLNDIY
                     RNKSVIQREELTQQLAQLVVKTMVDYWSLAIAEENVRTFDLLLSNTRNIRDITIGKAN
                     LGLAEGFEINQWSALTAQAESQQAGAILERNSRRRELLRTLNLDPTLELSGAATLEDK
                     IPTDLDEARDIAYAKEHRPDIRNVRLQMENATLASEIADNSMLPSMYVGGKYSSRDQG
                     RHTITAFNEVPHATYPESGIEFRVEMPLWDDGARVDARNARISLKQMAIQQKQLDRQV
                     EDEIRDNLDRIKTSFDAMKKAESGVEQMTYFYTGLLVRYRQGRFTAVAVKNALDSLVQ
                     AKFALTQARINFNISLVRYELTRNTVWKKYNVDIGEVIDRLKKD"
     gene            complement(8735..9649)
                     /locus_tag="PLG78_04355"
     CDS             complement(8735..9649)
                     /locus_tag="PLG78_04355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE22369.1"
                     /translation="MHRYSSNFFDAACIVPVRARPELLAQAVISILKGSTLPREIILV
                     LDGTAEERAADQNGVKQITHSAEAASVRLRVLETPAVGPAAARNTAVRATEMPWLTFL
                     DSDDLWETRKLEMQSTYMRKRPHLLACQTGESWMKNDKPVHRPRKLTPHTGRFLRDAV
                     HSCLLSLSAFMIRRDAFLDAGMFDPDFALCEDFDFSLRFLRSHAIGYLEEDLVEKRSG
                     SWKQLSQTPAIDAYRVRALCKLLGDHGRAGEGPGSIAERERSEAIRSLTHKVAILEKG
                     QEKFGQSAFVQNAIREGRAVLSLSSDDR"
     gene            9696..>10537
                     /locus_tag="PLG78_04360"
     CDS             9696..>10537
                     /locus_tag="PLG78_04360"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNE22370.1"
                     /translation="MDIFEATTAYESWLRTVTEVDSEALQVKHKAMCDGAFRFLRAAY
                     YDWIALWQSMPAEIKAAPVCPSIGDIHIENFGTWRDAEGRLIWGINDYDEATELPYTN
                     DLVRLATSVLLAIEDDRLEMKPRESCEAILSGYTRGLERGAKVFVLAEDNDWLRDIAR
                     RQIKDPGKFFRSMESLPDAAPPARVIEILKGILPEPFRVKKRIAGLGSLGRQRYAAIA
                     EVAGSRIVREVKANCPPSQNAFGSVAESLRARILENRPTSPDPFLLDASDYTVRRLAP
                     DCTK"
BASE COUNT         2832 a         2665 c         2699 g         2341 t
ORIGIN      
        1 atccccagag tgcgaagaag cgtcatcccg tcaaaatcgg gcatcttcag atccaggagg
       61 atcagatcca cgcgatcgtg actggcggcc ttcatggcac cgtccgcgtc cgcggcagaa
      121 actacgactg cccccgcttt cgtcagagca tgcgatacca gggcgcgcgt gctttcgtcg
      181 tcgtcaatga ccaggattct cttccctttc aaaacggcct cacccggtaa gtatcggact
      241 ggtccgccct gtcaagtgag gccacagcag tctcaaaaaa tcttccggct cacacaatcc
      301 acacacttcg ctcatactgc gtccactcga gcgtgcgatt ctgtctgtat caaaggagat
      361 agatatgatt cgacttacaa tcaaaatggc gctgatcggg gctctgtcca cgatgttgct
      421 cattcccaca gcatacattt gttctcttac aaaagagaga aaggacagaa gagacgaagt
      481 ggccaggagc actggaaacc tctggggcag ccatcatgaa atcgggacca ttctggaggc
      541 cggaaaagag agcatcctcc ctcaaagcgt ggatgtgaca ggaagcgttc tggcagaaac
      601 cagaaatcga ggcatctact cgctgccgtt ctatagcgcg gacctcacga tgcaggtgca
      661 attctttctg gaacgcgaaa gccaggcgtc ggtgattcga ctctacgggg taaaagcgag
      721 tgaagcagat ttccttacat ctaagttaga cgatcttgag attcgcttta aaggagatga
      781 tgaaccgagg caggtggcgg cccacgcagg aaccctttcg cgcggcactc acaaacttac
      841 gattcatttc aaaatgaacg gaacccagag actgcacttt gttccagcag caaatactct
      901 gacggtagat ctcaaatcaa actggccgca tcctaatttt tcaggatcga tcctgcctgc
      961 agaaagaacg atcgatgcaa atggcttcca tgcaaagtgg atcgtcaagc cgcagaatgc
     1021 aagcgccttc ctggcagagc tcaagaacaa cgaatacaga gaaacgctga cggaaggaga
     1081 agaagcaacg agcttcggag tggacttctt tgtgccagta gatatttacg cgcaaattga
     1141 acgagcgtcc aaatacgcga tgctttttat atcgctcaca tttctcacgt ttttcgtttt
     1201 cgaagcggtg gcggggcttc aaattcatcc tgtgcagtac cttttcgtag gcctggcttt
     1261 gagtctgttc ttcctgcttc tactgtctct ggcagaacac attggatttg cgcgagccta
     1321 tgggctggcg tctgttgcag atcttggcct cattacggcc tatagcttga agattttgaa
     1381 atccagaacc agagctggca ttttgctcgg cgttctggca ggactttatt ccttcctgta
     1441 tgtggtgttg cagctagaaa tgtattctct gcttgcagga tccattggcc tgttcttaat
     1501 cctggcaatt gtgatgtatg caacccgcaa catagactgg tacgcaaagg aaccagcgaa
     1561 agcgtaagac attcttccat tgaaccaacc attgccgccc ttcgcggggc ggcttttttt
     1621 atgcccgcgt gacctccccc tcgattactt gaccccgaag ccgtccgggc tctgtggaac
     1681 ctatgaactt aaactggaat tcaaagcaaa tcacacaagg agatcgccga gcgcccaacc
     1741 gtgcgatgat gcgtgctgta ggttttgagg acagcgactt tgacaaacca gtcgtaggtg
     1801 tggcaagcgg tcattccaca atcactccct gcaacgcagg catacgacct ctggaagaag
     1861 ctgcatgcaa agggatttca tccgctggcg ggatgcccca gacgtttggc accataacga
     1921 tttcagatgg aattagcatg ggcacccagg gaatgaagct ttcgctcgtg agtcgcgacg
     1981 tcatcgcaga cagcattgaa acagtatgtc gcgggcaact catggatggt gtgctggcca
     2041 ttggcggctg cgacaagaac ctgcctggtg cgatgatggc gatcgcgcgt ctggacattc
     2101 caggaatctt tgtatatggc ggaaccattc ttccaggaaa ataccagggg cgcgatctta
     2161 acatcgtttc tgtattcgag gcggttggtt cttttcaggc aggcacgatt tcccgcgaag
     2221 agctaaatga aattgaaaag cacgcctgtc cttcttctgg aagctgtggt ggaatgtaca
     2281 ctgcaaatac aatgtccagt gccattgaag cgctcgggat gagcctgccg aactcatcgc
     2341 agatgtcagc accgtcccct gaaaagaaat catctgcgga agaaagcgga gaggcactcg
     2401 ttcgcatgat tcgaaccaac ctgcgcccgt ctcagatcat gacgcgaaaa gcattcgaaa
     2461 acgcaattgc agtagtcatg gccacaggtg gctccacaaa cgcagtgctt catctgatcg
     2521 ccattgcgca tgatatggga ataaccctga cgttagacga ctttgaagcg atttctaaaa
     2581 aagtccctca cctggcagac atgaggccat ccggcaaata cctcgcagtt gattttcata
     2641 gagcgggtgg agttcctctc atcatgaagt tgcttctcaa ggcgggaatc cttcacggtg
     2701 aatgcatgac tgtgaccggg cgaactgttt cagaaaacct ggcggaagtt ccagatgctc
     2761 ctcccgcggg ccaggacgtg attcgacctt tttcttctcc tgtgtataag agaggtcacc
     2821 tggtcatcct tcgtggcaat ctggctcctg gaggagcagt tgcaaaaaca tctggagtca
     2881 aaagccccgc gatcacagga gaagcgcgcg tgtttgattc agaggaagag tgcatggacg
     2941 ctatcatgag cgatcaaatc aaaccgggag acatcctggt cattcgatac gaaggtccaa
     3001 agggtggccc tggaatgcgc gaaatgcttt cgcctacttc tgctctcatt ggcaagggtc
     3061 tcggcgattc agttggcctc atcacagacg gaagattctc aggcggcacc tatggaatgg
     3121 tagtcgggca tgtttctccg gaagcggcgg tgggtggacc catcgcactc gtgcgcgaag
     3181 gcgacgtgat cacgatcgat gcatacgaaa atctgcttca attgcacatg acagaggcgg
     3241 aaatggaagc acgcagaaaa gaatgggtaa aaccagaatc gccggtaaaa aatggagtgc
     3301 tctacaagta tgctaagctc gtgggaagcg ctgaattcgg ggcggttacg gatgcatgat
     3361 ttttgagtgc acgggagccc tgggtcccgg aaaaatgact caggcgccat atgaaaaccg
     3421 atcacctcat tctggacgaa tccggcaaac agactagaga caaaccctgg atatttcgca
     3481 catacgccgg gcacacaagc gcccgcgcat ccaacgaact atacagaacc aacctggccg
     3541 caggccagac aggcctgtcg atcgcctttg atctggccac tcagtgcggc tactcttcgg
     3601 atcatcccat cgcaaaaccg gaagtaggaa aagtgggagt gcccatcaac agcctcgatg
     3661 actttcacat tctcttcgat cagattccac tcgagaagat gaatacttct atgaccatca
     3721 acgccacgtc catgtggctt ctgtctctct acattgcact tgcgcgcgaa aggaatgaaa
     3781 gtccaaaaaa attgcagggc acaactcaaa acgacattgt caaagagtac ctggcgcgcg
     3841 gcacatacat tttccctcct caggcctctc tgcgcatgat cgcagagatg tatgaatact
     3901 gtttgaaaga agttccagaa tggaaccctt ctaacatttg ctcttaccat ttgcaggagg
     3961 caggtgccac tcccactcag gagcttgcat tcgctctggc caatgcaatt gggctgctcg
     4021 atctcatccg agaaagagga acgttcacgc atgacgaatt tgaaaaatgc gtgggaagaa
     4081 tcagctactt cgtaaacgca gggattcgct tcgtggaaga aatgtgcaag atgcgcgcat
     4141 tcacggagct gtgggaagaa atcactcgag atcgctacgg agtgaaaaat gcaaaatata
     4201 gaatgtttcg ttacggcgtg caggtaaatt ccctcgggct cacagaagcg cagccggaaa
     4261 acaacgcgtg gagaattctc atagaagctc ttggtgtaac catgagtaaa cgtgcacgat
     4321 gccgtgctct tcaactccct gcgtggaatg aagcgctgtc gcttcctcgt ccgtgggacc
     4381 agcaatgggc cttaagactc cagcaaattc tcgcatacga aacagacctg ctcgaatatc
     4441 cggatctgtt tgacggaagc aaggtagtag aatcaaaagt agaagcgctg aaagaagaat
     4501 gcagggcgga actggataag attctgggaa tgggcggagt gcgcgcggcc gtagaaagcg
     4561 gttacctcaa aacgtctctg gtcaaatcca tgtcagagag actgagcaaa atcaattcag
     4621 gcgaactgac tgtagttgga atcaacaaat ggaaggacgg cattgcaagt cctctcacaa
     4681 gcggaaatga tggcggcatc ttcaaagtag atccagcaaa agtcaaagag accatggaca
     4741 acctggaagc caccaaacgc aaacgcgacc cggcaaaagt agaagcagca ctcgccaaac
     4801 tcaaagctgc cgcaaagagc ggagaaaaca tgatggagcc atccattgaa tgcgctcttg
     4861 ctcgcgtaac caccggcgaa tggtcagacg cacttcgcag tgtttttgga gaatacagac
     4921 ccgcgacagg aatcgacggg cagaagctac ttctagaaga tgaacgtgtt gcaagcgtga
     4981 gaaagagagt agaaacattc actgcaaaaa gaggtcacag accgcgcatc gtagtcggaa
     5041 agccgggact cgatggtcat tccaacggag cagagatgat tgcagtgaca gcgcggcatt
     5101 ctggttttga tgtaatctat tccggaatca ggctgactcc cgaagaaatc gtgcagagcg
     5161 ccgtagaaga gaacgcggac atcattggca tttccattct gagcgggtcg catattgagc
     5221 tggcagaaca gatagttgca aaactgaagg aaatgaaagc agagcgaatt ccggtggtat
     5281 tcggaggaat cattccaaaa gaagatttcg ctgttctgga aaagacagga attcgcaaac
     5341 tctttactcc ttctgactac aagctcatgg atatcatgga ttccattttg aatgtggtag
     5401 aagaggagtc tgcgaaatag agaagtatgc tcttgcacgg ctgatctctg attttgaaaa
     5461 tacgctgccc gaagcggcac tgcgccggca cgatcatatc gaaaggctga aatcgcttcc
     5521 agaagcgcgc aaaggtgtat tcgcaggttt cactggcact ccaggcgccg gaaagtccac
     5581 tctcatcggt gcgctttgcc tcagactcat agagatggat ccgcacatca gagtggctgt
     5641 cgtggctgta gatccttcga gtgaagtttc aggcggagcg cttctgggag atagaactcg
     5701 tgttcgattt cctgtagatg aggacagact tttctttcgc agccaggcct ctgacagaca
     5761 actcggtgga atgagcccgg gcacgtttca ggtatgcagg cttctttatc atctgtttga
     5821 tcttgtaatc attgagaccg taggaattgg tcaaaacgaa atagaggtgc agagactcgc
     5881 agatcgagtc tatctcgtct tacaaccgtt agctggagat caaattcagt ttttgaaggc
     5941 tggaatcatg gagattccgg atgtttttct cttaaacaaa agcgatgaag tggaagcggc
     6001 cagaaagacc tatcacgccc tcaaagcaag cattcagttc acgaggcccg gggatggaga
     6061 cattcagatt ctacagacga gtgccgttat ggggacgggc attgacgaag tgtgcgcgga
     6121 tctgatggaa ctggtgcgaa agccccggga cggtgaatct ttgatgcgcg aaaaagaaga
     6181 aagatacttt tcccagtggg tccgcgaaga atacggaaga agtggaatca aaaaactcct
     6241 ggacctcgga ggtgcagcct tctggatcaa agaatgtgga agctatgacg cagccactcg
     6301 cagactgatt gacaagttca actgaagcaa gatgcaaatc gatttcttcg aagaatttcc
     6361 gtctgaagaa acgctgtcta aggcaaagct catgaatggt gagtttctag tttacatcgc
     6421 aagctctacc atttctgatt ttcatcgcca ccgcgacatg ctctctagaa tttcttcgaa
     6481 tgcgaaggct gcctactggc ccttgctccg tcgatcttac tgggtttctc ctttctcttt
     6541 tccagaagaa ctggaaggcc tgtaccgcga tctggaaaac agctcgccca tggacgtact
     6601 gatcgatctg gaattacctc ttcttcggcc cgccctcttt ctcaaaaacg tgcctcattt
     6661 cctggaaaac aaaaaactca tccggaagat cctcggactg gggcatcgct tcctggctgc
     6721 agtttacccc gccactggaa ttctatcaga atccatcctg cgcgcactcg gcatctggca
     6781 tcctcagaca gaaagctgtg tgatgtatta caccagcatg atccaaaagc cggccatcat
     6841 ccgctggatg caatctgcga tccgaaggcg cgccctattg ggacatagaa ccgttgggct
     6901 gggatgcctg gcccctggta tatttgggac ggaaccagtg ataacaactg agggtctaaa
     6961 gcgagatatg gatttcctca cagaagcagg ggtaaaacga gcaattttat tccgcctcgg
     7021 tggactcgat tcagaatttg caagtatcct ttgcgggaat aaaaaaatag cactgccggg
     7081 cagtgaagaa aaacaaggca tatgcgccga ttgggactga ttctattcgt gttcggcata
     7141 tctgccttga tggcccagga cccggcgggt ccagcccagg atgaaatgaa gacgagcgaa
     7201 actgctcgac cggccgcctc cgattcaaag caggcaacca ccactcccgt ttcgcagaac
     7261 aacgcgcagg cgacgtacag caaagtcccc actggaccgg acggaaaacc tctgcctctt
     7321 tccatggaac agattgtgaa gatggtcctc gaaaacaata atctggttcg catccagcag
     7381 cttgaaattc ttaaatctga tacggacatc atgaaagatg agtccaagta tgcgcccaaa
     7441 attggcgcgt cctacgaagg ctatgagaaa ctcgataagt cttctgcatc caccatcttc
     7501 agtggagaca aagtaaacca ggacaagtac accgcgtaca tgaacaagct gttctcgagc
     7561 ggtacttact tccaggtgga agcatccgac actcgctttg attccaatgc gggagaatct
     7621 ttcgcactgc agggaaccac ttttgcgaag ctcgctcaac ctcctctgca tactggagct
     7681 ctgactctca ctctgcgcca ggaacttctg aagaatgcat ttggatacag ccagcgcaga
     7741 ctcaatgaca tctatagaaa caaatctgta atccagcgcg aagaactgac tcaacagctc
     7801 gctcagctcg tggtcaaaac catggtggac tactggtctc ttgccattgc agaagaaaat
     7861 gttcgcacgt tcgacctgct attgagcaac acgagaaaca ttcgcgatat cacaatcggc
     7921 aaagcgaatc ttggcctggc agaaggattc gagatcaacc agtggagcgc cctgaccgca
     7981 caggctgaaa gccagcaagc aggcgcgatt ctagaaagaa acagccgcag aagagaacta
     8041 cttcgcacac tgaacctgga tccaactctg gaactgagcg gtgcagccac tctggaagac
     8101 aagatcccaa cggatctgga tgaagcgcgc gacattgcct acgcaaaaga gcatcgccct
     8161 gacattcgca acgttcgtct tcaaatggaa aacgcaactc tggcaagcga gattgcagac
     8221 aattccatgc tgccctccat gtatgttggc gggaaatatt ccagccgcga ccagggcaga
     8281 cacacgatca ctgcattcaa tgaagtccct cacgccactt acccagaaag tggaatcgaa
     8341 tttagagtgg aaatgcctct ctgggacgat ggtgcgcgcg tggatgcacg caatgcgcgg
     8401 atcagcctga agcaaatggc catccagcaa aagcagctcg atcgtcaggt ggaagacgaa
     8461 atcagagaca acctggatcg catcaagaca tcctttgatg ccatgaagaa agcagaatca
     8521 ggcgtagagc agatgacgta tttctacact ggcctgcttg tgcgatatcg ccagggaaga
     8581 ttcacagctg ttgcggtaaa aaacgcactc gactctctgg ttcaggcaaa gttcgcactc
     8641 actcaggcac gcatcaattt caacatctca cttgtgagat acgaactgac gcgcaacaca
     8701 gtgtggaaga aatacaacgt agatatcggg gaagtcatcg atcgtctgaa gaaagactga
     8761 gaacggcgcg tccctctcga atcgcattct gaacgaatgc gctctgtcca aatttttcct
     8821 ggcccttttc aagaatggcc actttgtgcg tgagagatcg aatggcctcc gatctttcgc
     8881 gctctgcaat tgaacccggc ccttctccag ctcgcccgtg atctcctaac aatttgcaca
     8941 gtgctctgac tctgtatgca tcgatcgcag gagtctgact gagttgtttc caggagccgc
     9001 tccttttttc cacaagatct tcttcgagat atccaattgc atgagaacgc agaaatcgca
     9061 gggaaaaatc aaaatcttcg cacagggcaa aatcaggatc aaacattccc gcatccaaaa
     9121 acgcatcgcg gcgaatcatg aacgcagaca gagaaagaag acaactgtgc acagcatctc
     9181 tcaggaaacg tccagtatga ggagtgagct ttctcggcct gtgcacaggc ttgtcgtttt
     9241 tcatccagct ttcgcctgtc tggcaggcaa gtagatgcgg acgctttcgc atgtatgtag
     9301 attgcatctc gagcttgcga gtctcccata gatcgtctga atcaagaaac gtaagccagg
     9361 gcatttctgt ggcccttacc gcggtgttcc tggccgcggc cgggccaact gcgggcgttt
     9421 ctaaaacgcg cagacgaaca gaggctgcct ctgcgctatg tgtaatctgt ttgactccgt
     9481 tttgatccgc agctctctct tccgcagttc catcgagcac gaggatgatt tctcgtggca
     9541 gagtgcttcc tttcaagata gatatcactg cttgcgccag gagctctggt ctggctctca
     9601 ccggaacaat gcaggctgcg tcgaaaaaat tagaactgta acgatgcaat tgtatttccg
     9661 cttgagcagg cggccctggc aaaccacaac cagctatgga tatttttgag gccactaccg
     9721 cctacgaaag ctggctgcgc actgtcacag aagtggattc agaagccctt caggtcaaac
     9781 acaaagcaat gtgcgatgga gcatttcgct ttttaagagc tgcctactac gactggatcg
     9841 cactctggca atcgatgccc gctgaaatca aagccgcgcc tgtctgtccg tccatcggag
     9901 acatccacat tgagaacttt ggcacatgga gagacgcaga aggaagattg atctggggaa
     9961 tcaatgacta cgacgaagca acagaacttc cttacaccaa tgacctggta cgcctggcaa
    10021 cgagtgtgct ccttgccatt gaagatgaca ggctggaaat gaagccacgc gaatcgtgcg
    10081 aagcgattct ttccgggtac acgcgagggc tcgaaagagg cgcgaaggta ttcgttcttg
    10141 cagaggacaa tgactggctc agagacattg cacgcagaca gatcaaagat cccggaaagt
    10201 tttttcgttc catggaaagc cttccggatg cggcccctcc tgcgcgtgtg atagaaattc
    10261 taaaaggcat tctgccagaa ccgtttagag tcaaaaaaag aatcgcaggt cttggaagtc
    10321 tgggaagaca acgttatgcg gccatcgcgg aagtagccgg atcgagaatt gttcgtgaag
    10381 taaaggcaaa ctgtccgccg tctcaaaatg cattcggcag cgtcgcagaa agtctgcgcg
    10441 cccggatcct cgaaaatcgt cccacaagtc cggatccttt tttattggac gcaagcgatt
    10501 acacggtccg aagacttgcg cctgactgca caaagat
//
LOCUS       DAOQJL010000062        10486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3289_length_10486_cov_5.033074, whole genome shotgun sequence.
ACCESSION   DAOQJL010000062 DAOQJL010000000
VERSION     DAOQJL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_10486_cov_5.033074"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="PLG78_04365"
     CDS             <1..1885
                     /locus_tag="PLG78_04365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22371.1"
                     /translation="SEEIRFDTLLEKLLSIMMENAGASRGAIVIEDRGHLTVEVVMDA
                     GQTTNQVTQSTALASAKALPLRVVYFVQRTRRSVILENAYRNSEYGSEPYIASHHVRS
                     LACLPIVNRGKLQGIIYLENRHTAGTFTSERLEILGFLTGQAAISIENAIVYRDLEAK
                     VSERTAMLNEKKSELEKSLSVVEKLKSEQDCDYYLTSLLLEPLAASRVDTGEIRIDFY
                     LEQKKKFRYRRWESSLGGDICSAHTIELRGARYSVFVNADAMGKSIQGAGGALVLGAV
                     FASLIQRTKLTRQIQEQYPEQWLKFSFVELQKVFESFEGYMMISMVLGIVNNRTGAAY
                     FINAEHPDLVLLRHGQATFLQPVATYAKIGRRIGGRGENWSVLRDDVPLSATCFQLQP
                     GDMLFAGSDGRDDIGLGLDDAGNTIVSENPDLFLRKVEESEGSLERLQGALHEAGEIL
                     DDLSLLKIEYLGHGLAGDLGTEVERELSKKIRGATSLEALRSVLLWFNEAALDREPSL
                     VGLFARYRMEMRLGLTDAAIDSLQRYVERNHLDTRLMLVLAKLQARADGATALATLDR
                     LRLRDPLNNRFTRRYIHTLIMVGGYEEELKTAINDWRKRVGKGKREQKLLAGVSSPRM
                     TRG"
     gene            complement(1866..2612)
                     /locus_tag="PLG78_04370"
     CDS             complement(1866..2612)
                     /locus_tag="PLG78_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE22372.1"
                     /translation="MNSLFDVQGKVVLITGASRGIGKEMATAFADAGAIVYGTASRPE
                     SISQGHNPAIQMSACDVRDRGMLKDLMLRIKATHGRVDALINNAGITSSSLASQVSDE
                     EMQSLFETNLKGLFSACQVYYSTQKREGCIINISSASAMRAMPGTSFYGSIKAGVIQT
                     TQALAVEWARRNIRVNCISPGFIKTDMNAHLRQNESTAAQIAASIPLGFIGEPADVSG
                     AAVFLVSNAARYITGHNLVIDGGALSRASF"
     gene            complement(2609..3742)
                     /locus_tag="PLG78_04375"
     CDS             complement(2609..3742)
                     /locus_tag="PLG78_04375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNE22373.1"
                     /translation="MTSLNGIRVLDLSAVFPFPMAAAYLADLGADVIKIENPRLPDPS
                     RKMGSPSGSTHDGSIFVLANRNKRCLSLNLKRPRGMEVFKKLLANTDVLIESFLPGTT
                     SRLGITYDDLRDSFPRLVYCHGSPYGYGQSSRPAHEINIAAEAGLIATTGTASTPVPP
                     GFQPSDVAGAFCAVGGILAALLARHQTGRGQFVNASLFDGAMSMLSLVAAEHLAGGNT
                     DRGKMDVSGMAPGYRVYQTREGFIALGTLEDAAYEKFLNYIDRKHIKNMPEESQIAEL
                     SRYFLDCDSSSIQRLIDAELLVSRVNSVAEAMTSARLRTPTLLQRMPQGGETLGAPFH
                     LSETPVSIRSGVPERGEHTRDILKTLGYSQAEYEELQNEGVVA"
     gene            complement(3739..6165)
                     /locus_tag="PLG78_04380"
     CDS             complement(3739..6165)
                     /locus_tag="PLG78_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22374.1"
                     /translation="MGSKPLAILIISDDRAFIEDSAGVFMNLAGAGHAQVQCDFENTQ
                     LDIGRMAWLTDSPVYRNAQIVAQSFLLAHTTPPILVFQASTFQEAVTRITAKHRESEF
                     ALGMIVLDETTVGDLSYERTGLIQAFDDFYSQLGTAGVEWIRSSYSIIAYTAAFPFLS
                     GGAYLPPLRYLYRVRPQDTSLLRADLLCCFMDFYELTFLNTRARRGEKNRDNVTLALE
                     LVAFLNNRSQPREWDCYYYTGSIVSSLIETIEKALYQQNQFCFMGPNEHSLACGAMAN
                     WKLYARPFLIVVTSAMADEFKGTLANLREARAKGFIVVAENREDAWFPFQGTRTQEEN
                     MMDVIKAKKLPAVYLKDSETLATDLQTAFEFYESGEGPVFLFVTQQVLQSKQPLSVAA
                     DQLYPKPKVVVPAPSSPQTEQDFEKIVRLLNEEKTRIIWQVGQLSEEEYNLTIEIADA
                     AGLALCDNLSRPGNVSKYKNGQLVPQYLGTLSLYGFSQRAFRYMHNHGKLNPKDSQVL
                     FFFKSKISQINSPFSESAMKNKLRIGQISANPTHLARFVDFPCAMDSLEFLRKLRRAL
                     NVKPEVRAFREETLKQLPFTTGDIVSKVATQPLHPNYFFRELNQIVEELIVKEGYDYT
                     GVYDVGRNGVSGYRNVARTRPGFSGWYGRALMGDALLSVNALAVTSPVNILAFVGDGA
                     RGVVPDIRASMVENIAHMYRPGAFRKNITIFYMINGLVGLINTYQERMMSHRARRQMR
                     CVSLLDPDVKIKVGDLTVQQRNLYSFDADFIRESLLAPGQLNFFSVITNHNNTGDGMS
                     LLAADNWQEI"
     gene            complement(6289..7545)
                     /locus_tag="PLG78_04385"
     CDS             complement(6289..7545)
                     /locus_tag="PLG78_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22375.1"
                     /translation="MNDWCSGIAAKNLKRRSELVATVGQHSFTWARRIHALSRRPGGV
                     AEVIILNCSLLKNRSAKSPIIAFAHKKIHDAFTAVELLVSREIPMPAQTVIAQGGLFS
                     GMYVYRDWVPDFLKFQPLRFFSETFARILGNKMNRLFRDMNCHPVFRRFRDVPSRKVY
                     ESSRFSGRAISGMTYGEFLEYTRKETQQSISAVERDIRELNKSLIIFPEGKYQHSGAV
                     AKLNGFLMNVATESGHPIAAISQSYDELCPDSKRRIDALLTVSWIEPGETRVALSKKT
                     AAALQTGTAIVASQLIAWLLLQANRENKKEGITGSPAAPDAVNHLRLEFLRMCEEAKR
                     SGVPMDPRLHDRGFAEDRFARFMRANGDYVYEIDSKIVINQKKLRSFSKSERTVNNLE
                     WNKNNIIHLEGQLSAWFASSETSKDL"
     gene            7610..8122
                     /gene="tpx"
                     /locus_tag="PLG78_04390"
     CDS             7610..8122
                     /gene="tpx"
                     /locus_tag="PLG78_04390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNE22376.1"
                     /translation="MASVTHRGNTIQLDGNLPRPGEKAPGFKVVKDDLSEVGLESFAG
                     KVKVIVALPSLDTPVCQKETREFNQKLGSMKDTVVLVVSGDLPFAMKRFCSTEGLSNV
                     VTGSQFRDHAFSHAYGTFITEGGLKGLSARAVFVVDKNDTVQYTELVPDIGGEPDYNA
                     VSQAVSKLVG"
     gene            8122..8703
                     /locus_tag="PLG78_04395"
     CDS             8122..8703
                     /locus_tag="PLG78_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22377.1"
                     /translation="MTVLSDLRMIFSDWQAGLRDFLGRPAEPSRYYFLKNHLLLCIHA
                     PLFAALAPLPWIRGGHFSYSMRLILPFVLPIFFVALAAVLERIAIYERGPSMEEPRGK
                     NALLYLTLPACSAGIFYFVHPLLGILLTLAFGMFSIVACIRAAMEIWSLSFDRVLFRL
                     AGAFLFLMTPVLALLAIRNVLQSLGILNRLIHG"
     gene            8706..10106
                     /locus_tag="PLG78_04400"
     CDS             8706..10106
                     /locus_tag="PLG78_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNE22378.1"
                     /translation="MKIHITGVGGVAMGNLAAMLKYIGHDVTGSDRDLYPPMSERLKE
                     WKIEAHNFDSANVKGMDLCIIGNVISRGNPEVEEILNGGIPYMSLPQALYEFFLKGRR
                     VIVAAGTHGKTTTTFLTHYILSQAGIKAGLFAGGVRADGHDGFALPESDIFVIEGDEY
                     DSAFFDKGSKFLHYRPRYLILGAVEFDHADIFRNYEEYALPFRRLLRMIPSNGLVVAN
                     QMSDGVRDVLTDYRYSPIEWIANQSKKGVFGFHRRAETLSLDGIGDIRGNRLMGDYNA
                     RNALAASRIALALGVSPKQIRDAVASFPGVMRRQQIRLEANIDGVPVTFIEDFAHHPT
                     AVSETIDAVRQTYPDRSVSVLFEPRSATSHRNVFQKEYGAAFKRADAVYICEIHNKKK
                     VGVKDRLDVKKILAGLKVKTKEFGRDPDHLLTRFTSTFKVGKEGSVVLVLSNGAFGGI
                     YQKIETFLRDRSRRVR"
     gene            complement(10143..>10486)
                     /locus_tag="PLG78_04405"
     CDS             complement(10143..>10486)
                     /locus_tag="PLG78_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22379.1"
                     /translation="SRWIKAPRFLLTLRGKPKTNLRIEDISATNRPNIRDYGSYEKQG
                     AVPVGRKAELIQEIETSAETLKAAVRKRSEEELGKLSGLWGGMPLGIFAQFALKHGLH
                     HASVVKARMHG"
BASE COUNT         2481 a         2537 c         2725 g         2743 t
ORIGIN      
        1 ctccgaggaa atcagatttg atacattgct tgagaaattg ctcagcatca tgatggagaa
       61 tgcaggtgca tctcgcggag ccatcgtaat cgaagacaga ggacacctga ctgtagaagt
      121 cgtgatggat gccggtcaaa caaccaatca ggtgacacaa tccacggcac ttgccagtgc
      181 gaaagccctg cctcttcgcg tcgtttattt tgttcagcgc actcgtcgta gtgtcattct
      241 agagaatgcg tatagaaact ctgaatatgg ttcagagcca tatattgcgt cgcatcatgt
      301 gcgatctctt gcctgtttgc caatcgtgaa cagaggaaaa cttcaaggca tcatctatct
      361 ggagaatcgg catacggccg gcaccttcac ttccgaacga ctcgaaatcc tgggctttct
      421 cactggccag gccgccatct cgattgaaaa cgccattgtt taccgcgatc tagaagcgaa
      481 ggtgtccgag cgtacggcaa tgctcaatga aaagaaaagc gaactggaga aatcgctttc
      541 cgtagtagaa aaactgaagt ctgagcaaga ttgtgactac tatcttactt ctttgctact
      601 tgaacctctc gccgccagtc gcgtcgatac tggagaaatt agaatcgact tttatctgga
      661 gcagaagaag aagtttcgct atcgcagatg ggagagttct cttggcggag atatttgcag
      721 tgctcacacc atagagttac gaggcgcccg ttattcggta ttcgtcaacg cagacgcaat
      781 gggcaaatcg attcaaggtg caggaggcgc tctggttctc ggggctgttt tcgcatctct
      841 gattcaaaga acgaagctga ctcgacaaat ccaggaacag tatccagaac aatggctcaa
      901 attttcgttc gtagagcttc aaaaagtttt cgaaagtttt gaaggatata tgatgatttc
      961 catggttctt ggaatcgtca ataaccgaac aggcgcggct tacttcatca acgcagaaca
     1021 tccggatctt gttctcttgc gtcatggtca ggctactttt ctacagccag tggcaacgta
     1081 tgcgaagata ggaaggcgca ttgggggacg aggggaaaac tggagtgttt tgcgcgatga
     1141 cgtacctctc agcgctactt gctttcaact tcagccggga gacatgctct ttgccgggtc
     1201 agacggccgc gatgacatcg gtcttggtct tgatgatgcc ggcaatacca tcgtgagcga
     1261 aaatcctgac ttgttccttc gcaaagttga agagagcgaa ggatcgctgg aaagattgca
     1321 gggggcgcta catgaagcgg gtgagatact ggatgatctt tcgcttttga agattgaata
     1381 ccttggtcat ggactggccg gggaccttgg aacagaagtc gaaagggaac tctccaagaa
     1441 gattcgcggc gcaacaagtc tggaagcatt gcgttccgtg ctgctctggt tcaacgaagc
     1501 ggctctagat cgagagcctt ctcttgtcgg gttatttgcc agatatcgca tggaaatgag
     1561 gctgggtttg acagatgccg cgatcgacag tctacagcgc tatgtggagc gcaaccatct
     1621 ggacactcgt ctgatgcttg ttcttgcgaa acttcaggcc cgcgctgatg gagcgactgc
     1681 gcttgcgact ctggatcgcc ttcgcttgcg cgatcctctg aacaatcgat ttaccagacg
     1741 ctacattcac actctgatca tggttggcgg ttacgaggaa gaactcaaaa cagcaatcaa
     1801 tgattggcga aagagagtgg gcaaaggcaa gagagaacag aaactcctgg caggtgtttc
     1861 cagccctaga atgacgcgcg gctgagtgcg ccgccatcaa tcaccaggtt atgtccggtg
     1921 atgtagcgcg ctgcgttgct cacaagaaac acggcagctc cagaaacgtc cgctggctcc
     1981 ccgataaaac cgagtggaat ggaagcagcg atttgcgccg ctgtgctctc gttctggcgg
     2041 aggtgcgcat tcatatctgt tttgatgaag ccaggagaaa tgcagttcac tcgaatattt
     2101 ctgcgcgccc actctaccgc cagtgcctgt gttgtctgga ttacaccggc cttgatgctg
     2161 ccataaaaag aagtacctgg catagccctc attgcgcttg cggaggaaat gttgatgatg
     2221 caaccttcgc gcttttgcgt ggagtagtaa acctgacatg ctgagaagag acctttgagg
     2281 ttggtttcaa acaatgattg catttcttcg tcgctgactt gagacgcgag tgaagaagaa
     2341 gtgattccag cgttgttgat cagagcatcc acccggccgt gtgttgcttt aatacgaagc
     2401 atgagatcct tgagcatacc tcgatcgcga acgtcgcacg cggacatctg aatggcaggg
     2461 ttgtggcctt gagagataga ttctggtctg gaagcagttc cgtagacgat cgctcctgca
     2521 tctgcgaaag cggtggccat ttcttttcca atgcctctgc ttgctccggt gatgagaacc
     2581 accttgccct ggacatcaaa tagagagttc atgctactac tccttcgttc tgcaattctt
     2641 catactctgc ttgtgaatag ccaagcgtct taagaatatc acgagtatgc tcgccacgtt
     2701 ccggtacacc gctgcggata gacactggcg tttcacttaa atgaaaagga gcgcccagtg
     2761 tttctcctcc ttgcggcatg cgctgcaata gagttggtgt gcgaaggcgc gcgctcgtca
     2821 ttgcttctgc aactgaattt acgcgggata caagtaattc tgcatcaatc agccgctgaa
     2881 tagaagaaga atcgcaatca aggaagtaac gggagagctc tgcgatttgc gattcctccg
     2941 gcatgttctt gatgtgtttt ctgtctatgt agttcaggaa tttttcatac gccgcgtcct
     3001 ccagcgttcc aagtgcgata aatccttcgc gcgtttgata cactctgtat ccgggggcca
     3061 taccagaaac atccatcttt cctcgatccg tatttccgcc tgcgagatgt tcggcggcaa
     3121 ccagcgagag catagacatt gcgccgtcaa agagggatgc attcacaaat tgcccgcgcc
     3181 ctgtttgatg gcgcgccagg agtgctgcca ggattccacc tacagcgcaa aaagcacctg
     3241 caacatctga aggttgaaat cccgggggga ctggcgtgga cgcggtcccc gtagttgcaa
     3301 tcagacctgc ttctgctgca atgttgattt catgcgcagg ccgcgagctt tgcccgtaac
     3361 cgtacgggga tccatgacag tagacaaggc gcggaaagga atctcgcaaa tcatcgtacg
     3421 tgattcccag gcgagaagtg gttcccggaa gaaaactttc aataagaaca tctgtgtttg
     3481 caagtagctt cttgaagact tccattcccc tgggtcgctt gagattcagc gaaaggcatc
     3541 gtttgtttcg attggccaga acgaagatgc ttccatcgtg ggtgctcccg gaaggtgagc
     3601 ccatcttacg cgatggatcc ggaagtcgcg gattttcgat cttgattacg tcagccccga
     3661 gatccgccag ataggccgca gccatgggaa aaggaaacac agcagataga tcgaggaccc
     3721 gaattccatt cagcgatgtc atatttcctg ccagttatcg gctgccagta gactcattcc
     3781 atcgcccgta ttgttgtggt tggtgatcac agaaaagaag ttcaattgtc caggagccag
     3841 aagcgattcg cggataaaat cagcgtcaaa cgagtagaga ttgcgttgct gaacagtgag
     3901 atccccgacc ttgattttaa catccggatc caacagagaa acgcatcgca tttgtcttcg
     3961 cgcgcggtgc gacatcattc gttcctggta ggtattgatc aatccaacga gaccgtttat
     4021 catatagaag atagtgatgt tctttctaaa tgcaccgggg cgatacatat gcgcgatgtt
     4081 ctccaccatc gacgcgcgga tatcaggcac gacccctcgc gcaccgtcgc ccacaaaagc
     4141 cagaatattt accggactgg tgactgccag agcattgacg cttaagaggg cgtctcccat
     4201 gagtgcgcgg ccataccagc ctgagaaacc agggcgcgtt cgagccacat tgcgataacc
     4261 ggaaacacca ttgcgcccta catcatagac tccggtataa tcatatcctt cttttacaat
     4321 cagctcttct acgatttgat tgagttctct aaagaaatag ttcggatgca atggttgagt
     4381 cgccactttg ctgacaatgt ctccggtagt aaaagggagt tgcttgagcg tttcctcgcg
     4441 aaaggccctt acttccggtt ttacattcag cgctcttcgc agctttcgca gaaactcaag
     4501 cgaatccata gcacacggaa aatcaacgaa acgagccaga tgggtcgggt ttgcactgat
     4561 ttggccaatt cgaagtttgt tcttcatggc actttcggag aagggagaat tgatctgact
     4621 gatcttgctt ttgaagaaaa acagaacctg tgagtctttg ggattgagtt tcccgtggtt
     4681 gtgcatgtag cgaaacgctc gttgtgaaaa tccatacaac gacagagtcc ccaggtattg
     4741 aggaacaagc tggccgttct tatattttga aacatttcct ggtcttgaaa gattgtcgca
     4801 tagcgccagt ccggcggcgt ccgcaatttc aatcgtaaga ttgtattctt cttcgcttaa
     4861 ctggcctacc tgccagatga tgcgcgtttt ctcctcattg aggaggcgca ctatcttttc
     4921 aaaatcctgt tccgtctgcg ggcttgaagg cgcaggcacg actacttttg gttttggata
     4981 caactgatcg gcggccactg acagaggttg tttgctttgc agtacttgct gcgttacaaa
     5041 aaggaaaacc gggccttcgc cactttcgta gaattcaaag gcggtctgca gatctgtggc
     5101 aagtgtttct gaatccttga gatatacagc cggaagcttt ttggctttga tcacatccat
     5161 catattctct tcctgggttc ttgtgccctg gaaaggaaac catgcgtctt cacgattttc
     5221 agcgactaca atgaagcctt tcgctctggc ttcgcgcaga tttgcgaggg tgcctttgaa
     5281 ttcgtcagcc atggcactcg tcacaacgat gaggaagggt cgcgcgtaga gtttccagtt
     5341 tgccatagct ccgcaggcaa ggctgtgttc gttaggaccc ataaagcaga actggttttg
     5401 ctggtagaga gctttctcta ttgtctcgat gagtgaagat acaatagacc cggtgtagta
     5461 atagcagtcc cattcgcgtg gttgcgatct gttatttaga aaagccacaa gctcaagggc
     5521 cagtgtgacg ttgtcgcgat tcttttctcc cctgcgcgcg cgcgtgttca ggaatgtgag
     5581 ctcataaaaa tccatgaagc aacagagcag atcagctcgc aggagagatg tgtcctgtgg
     5641 ccgcactctg tacaggtagc gaagaggggg aaggtaggcg ccgccgctta aaaatggaaa
     5701 cgctgccgtg tacgcgatga ttgaatacga agagcggatc cattcaacac ctgcagtgcc
     5761 cagctgactg tagaaatcgt caaatgcctg gataagcccg gtcctttcat acgatagatc
     5821 tccgactgtg gtttcgtcga ggacgatcat tccgagagcg aattcgcttt cgcggtgttt
     5881 tgcagtgatg cgcgtgacag cttcctggaa cgttgatgcc tggaagacca gaatgggcgg
     5941 ggtcgtatgc gcgagtagaa aactctgggc tacaatttga gcatttctat acactggact
     6001 atctgtaagc cacgccattc ttccgatatc gagctgagta ttttcaaaat cgcactgaac
     6061 ctgcgcgtga cctgctcccg caaggttcat gaaaactccc gccgagtcct caatgaatgc
     6121 acgatcgtcg ctgatgatta gaattgctaa aggtttgctt cccatttttt ggccctgaat
     6181 ctgctatcat gggagagttt ctctggtcaa gcaacgaaag tttaacattt ccgacttctt
     6241 cgcgtcctga tcttgaaata cgggcacgta gtcgatgagg taatccgctt agagatcttt
     6301 tgatgtttcc gaggacgcaa accaggcgga cagttgcccc tccagatgaa tgatattgtt
     6361 tttattccac tcgagattgt ttacggtgcg ttctgattta ctaaaagatc ttaacttttt
     6421 ctggttaata actattttcg aatcgatttc gtacacgtag tctccgtttg cgcgcatgaa
     6481 tctggcaaaa cggtcctccg cgaatccgcg gtcgtgcaat cgtgggtcca ttggaacacc
     6541 agatcttttc gcttcttcgc acatgcgaag aaattctagt ctgaggtggt tgactgcatc
     6601 cggagccgca ggagaccctg tgattccttc tttcttgttt tctctgttgg cttgaagcag
     6661 aagccatgcg attaactgac ttgccacaat tgcggttccg gtttgaagtg cggccgctgt
     6721 cttcttgctg agagcaactc tagtttctcc tggttctatc caggaaacag tgagtaatgc
     6781 atcgattctt cttttggagt ccggacagag ttcatcatag gattgagata tagccgcgat
     6841 gggatggccg gactctgtgg ccacgttcat cagaaatccg ttcagttttg cgacagcacc
     6901 gctgtgctgg tatttgcctt ccggaaaaat gatcagagac ttgttgagtt ctcgaatgtc
     6961 gcgttcgact gcgctaatcg actgttgtgt ttctttcctc gtgtattcca ggaattcgcc
     7021 gtacgtcatt ccgctgatgg ctcgccctga aaaccgcgag ctttcgtata ccttgcgtga
     7081 gggaacatct ctgaatcgtc tgaacaccgg atgacaattc atatcgcgaa aaagtcgatt
     7141 catcttgttt ccaagaatgc gcgcaaatgt ttccgagaaa aaccgaagtg gctggaattt
     7201 tagaaagtcg ggaacccaat ctctgtagac atacatgccg ctgaacaatc cgccctgagc
     7261 aatcacggtt tgcgcgggca tgggaatttc gcgcgaaaca agaagctcga ctgcggtgaa
     7321 tgcgtcgtga atctttttgt gcgcaaatgc tatgatgggc gactttgcgg aacgattttt
     7381 caacaatgag caattgagaa tgatgacttc agctaccccg ccgggccttc tagagagcgc
     7441 atggatgcgc ctggcccagg tgaacgaatg ctggcccacg gttgcgacca gttccgagcg
     7501 ccgcttcaga ttctttgctg caatgcccga acaccaatcg ttcataaaaa gactggataa
     7561 tccagggcct ttttgggaat actatcaaaa aaaaatttag gagggtagca tggcttctgt
     7621 tacacatcga gggaatacaa tccagctgga tggtaatctc ccccggccag gcgaaaaagc
     7681 tcccggcttc aaggtcgtta aagacgatct ttcagaagtt ggccttgagt ctttcgctgg
     7741 caaagtcaaa gtgattgtcg cgcttccatc tctagacaca cccgtatgtc agaaagaaac
     7801 ccgtgagttc aatcagaaac tcggttcaat gaaggacaca gtggttctcg tagtatctgg
     7861 agatctgccg tttgcgatga agagattctg ttccacagaa gggttgagca atgtagtgac
     7921 tggttcgcag tttcgcgacc acgccttttc gcacgcttac ggaaccttca tcacagaagg
     7981 cgggctaaag ggattgtctg cgcgcgcagt gttcgtagta gataaaaacg acacagtgca
     8041 atatacagaa ctcgtgccgg atatcggtgg cgagccagat tacaacgcag tcagccaggc
     8101 agtgagcaag ctggtaggtt gatgaccgtt ctctcagatc ttcgcatgat cttttcggac
     8161 tggcaggccg ggttgagaga ttttctcggc cggcctgctg agccttcacg ctattatttc
     8221 ttaaagaatc atctgctttt gtgcattcac gctccgcttt ttgccgcact tgcaccgttg
     8281 ccctggattc gcggcggcca tttctcctat tctatgaggc tgattcttcc ttttgttctg
     8341 cctatcttct tcgtcgcgct tgcagcggtt ctcgaacgga ttgccatcta tgaaaggggg
     8401 ccttcgatgg aagagcctcg tggaaagaat gcacttctgt atttgacttt gcccgcctgc
     8461 tctgccggca ttttctattt cgttcacccg ctgcttggaa ttctactgac tcttgctttt
     8521 ggaatgtttt ccatcgtcgc ctgcattcgc gctgccatgg aaatctggtc tctttcgttt
     8581 gaccgggtgc tctttagact tgcaggtgca tttctattct tgatgactcc cgttctcgct
     8641 ctccttgcca ttcgcaacgt gcttcaaagc ctgggaattt tgaaccgtct catccacggg
     8701 taactatgaa gattcacata accggagtcg gcggagtggc catgggaaat ctggccgcga
     8761 tgttgaagta tatcggtcac gatgtgacag gttcagatcg cgatttgtat ccgcccatgt
     8821 ccgagcgctt aaaggaatgg aaaattgaag cgcataactt tgattcggcg aacgtcaaag
     8881 gaatggatct ctgcatcatc gggaacgtta tttctcgcgg gaatcctgag gtggaagaaa
     8941 ttctgaatgg cggcattccg tatatgagtc tgccgcaagc gttgtatgaa tttttcctga
     9001 agggaaggcg cgtcattgtc gcggccggca cgcacggcaa aacaactaca acattcctga
     9061 ctcactacat tctgtctcag gcagggatca aagccggttt gtttgcaggt ggagtaagag
     9121 cggacgggca cgatgggttt gctctccccg agtcagacat attcgtcatc gaaggggacg
     9181 agtatgattc tgcttttttt gacaagggtt caaagtttct gcactatcgt ccgcgatacc
     9241 tgatactggg tgcggttgaa tttgaccatg ctgatatctt tcgcaactac gaggaatacg
     9301 cacttccatt tcgacgccta ttgcggatga tcccgtcgaa cggtctggtg gttgccaatc
     9361 agatgtcgga cggcgttcgt gacgtgctta cggactaccg ttattcgccc attgaatgga
     9421 tcgcgaatca atcaaagaag ggagtgttcg gttttcaccg gcgcgcagaa acactgtctc
     9481 tcgatggaat cggggacata cgcggcaaca gattgatggg cgattacaac gctcgaaacg
     9541 cactggcagc ctctcgcata gccctcgcac tgggtgtatc cccgaagcaa attcgtgacg
     9601 cagtcgcctc ttttccgggg gtgatgcgac gccagcagat tcgtctggag gcaaacatag
     9661 acggagtgcc ggttactttt attgaagatt ttgcgcatca tccaaccgcg gtgtctgaaa
     9721 caatcgatgc cgttcggcaa acatatccgg atagatctgt ttctgtgctc tttgagccgc
     9781 gcagtgcgac ttctcatcgc aatgtgtttc agaaagagta cggcgccgct ttcaagcgcg
     9841 cagacgcagt ctacatctgc gaaattcaca ataagaagaa agtgggcgtg aaagatcgcc
     9901 tggatgtgaa aaaaatctta gcgggtttga aggtgaagac aaaagagttt gggcgtgacc
     9961 cggatcacct gctgacacgc ttcacatcca cattcaaggt gggcaaagag gggtccgtgg
    10021 tcctggttct ttctaacggc gcattcggag gaatctatca gaagatagaa acattcttac
    10081 gagatcgttc gcgtcgagtt cgctaagact ccagtgtctc aagcgcaggc ctgcgttatg
    10141 tgttagccgt gcatgcgtgc tttgactaca gaagcatgat gcaggccgtg tttgagcgca
    10201 aattgcgcga agatgccaag tggcattccg ccccagagtc cggacaattt gcccagttct
    10261 tcttcacttc tttttctcac cgctgctttt agagtttctg cggatgtttc aatttcttgg
    10321 atgagttcag ctttgcggcc aaccgggact gctccctgtt tttcgtagga tccgtagtca
    10381 cggatgtttg gccggtttgt ggcagagatg tcttctatgc gcagatttgt ctttggcttg
    10441 ccgcgaagcg tgagcaggaa gcgcggcgct ttgatccagc gactca
//
LOCUS       DAOQJL010000063        10433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3311_length_10433_cov_4.828098, whole genome shotgun sequence.
ACCESSION   DAOQJL010000063 DAOQJL010000000
VERSION     DAOQJL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_10433_cov_4.828098"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..558)
                     /gene="cobC"
                     /locus_tag="PLG78_04410"
     CDS             complement(43..558)
                     /gene="cobC"
                     /locus_tag="PLG78_04410"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HNE22380.1"
                     /translation="MDLYVVRHPPVAVDKGVCYGQLDVALKPGFEVLGNSLRESLPSL
                     PVYSSHLSRCRQLAHLIDPDHAVDARLAEMHFGEWEGLRWDDIPRTELDLWMNDYVTQ
                     RTPGGESFADVEDRLTEFLQTLRDAGKEGAVLISHAGVIRSLLIRWTGLSPDKAFRKQ
                     IEFGSVHHISV"
     gene            complement(543..1292)
                     /locus_tag="PLG78_04415"
     CDS             complement(543..1292)
                     /locus_tag="PLG78_04415"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNE22381.1"
                     /translation="MIRTFLTAVMFLTRIPCPSWIDHSEERLNRSVAYFPLVGAIVGG
                     ICAVVFLLANRLFSREISVVLCMIAGVLVTGAFHEDGFADFCDGFGGGWTKEKILEIM
                     KDSRIGAFGTIALILLFLLKYHVMIDLNSARLPALLIAGHSISRMFPVWIMKFLPYVR
                     DVSAVTKPIGVLTHGLDMVAATLFGLVPLAYFGRLYMLIPAVASAVTALLMALYMRKW
                     IGGYTGDCLGAVQQVTEVVFYGATLAIWTFM"
     gene            complement(1293..3566)
                     /locus_tag="PLG78_04420"
     CDS             complement(1293..3566)
                     /locus_tag="PLG78_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNE22382.1"
                     /translation="MMKKPLAVIAWICVALVGALGYIMVAMHRGEGLNAAWILTAALC
                     TYATAYRFYSKWIAAKILTLDDRRATPCEVHEDGRDFVRTNKWIVFGHHFAAISGPGP
                     LVGPVLAAQFGYLPGTLWILIGVVLGGAVQDFMILFSSMRRNGKSLGEMVQEEISKPA
                     GIVAMVAIVSIITILVAVLGLIVVKALAHSPWGTFTIAATIPIALFMGVYLRFIRPGK
                     VLEITTVGVILLLISVWAGKFFQQDPALGDVLTFSEPALAWMIIAYGLAASILPVWLL
                     LAPRDYLSTFMKLGTIGALALGIFLVLPPLTFPPLTRFVDGTGPVFSGDVFPFCFITI
                     ACGAISGFHSLISSGTTPKLITRESHARSVGYGSMLLESFVAIMALIAACTLSPGQYF
                     AMNASPSAVGKTPEVIVQTISGWGFPLTLQEMQVLAAELGEKTMFGRAGGAPTLAVGM
                     AHIFARLTGPALLALWYHFAIMFEALFILTTVDAGTRVGRFLLQALLGQIYKPLGNTH
                     SYGANVLGSVLLVSGWGYFLYSGVTDPLGGINSLWPLFGIANQLLSLVALCLATTILI
                     KMDKIKFLWVTLIPLTWLTAVTFTACLEKIYHPDPRIGFLSMAASLNDKLAHGQVPPE
                     KIGETGRIIFNMQLDALVAGAFLLLVGSILILSIIRWAGMLFGKGSRSLTETSPVWLS
                     NEVIAAEAAARPPWKFWMMGFFLAELFRHLSGQNRFEAARLSSDRSVERSSLAAVSAG
                     ETWAREEERRFKNPRCC"
     gene            3618..4502
                     /locus_tag="PLG78_04425"
     CDS             3618..4502
                     /locus_tag="PLG78_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNE22383.1"
                     /translation="MAISRVRIYEVGPRDGLQNEARIVSARDKIEFIRLLSLSGLTNI
                     EATAFVRSVSQMADAQEVYTSVRDFGPRMSALVPNRKGMENALASGLREAAIFTAASE
                     TFTRKNINSSIEESFSRFKEVMQLAADHAIPVRGYVSTVVECPYEGRIAPQKVAEICD
                     RLIQAGVYEISLGETIGVAVPDDISRLLDEVTKKVPPEKLAGHYHDTRGTALSNVFRS
                     LDYGLSVFDSSAGGLGGCPYAPGASGNLATEDLLYALHRSKIETGVDLGKIADASEFI
                     RHAIAKEPASRVFAAIRK"
     gene            4516..6249
                     /locus_tag="PLG78_04430"
     CDS             4516..6249
                     /locus_tag="PLG78_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE22384.1"
                     /translation="MDHLNLSEKLRLYAEAFEKALECMLIADENRRVIAVNPEFERVT
                     GYMLSEVLGKRPGFLDSGNHSLRFYRGIWRSVRDTGSWQGEIQSKRKNGELYSEWLSI
                     SQLHENGHIYYLAVFSDITHRKDYERTLYALANYDTLTGLPNRNLFSEILEKEVAEAG
                     RKNEKVGLLIIDVDRFKSVVMTLGHTTADELLIDIVHRIRTCLRRHDVLARLGGDEFA
                     VILPGLTESREASSIAREILAAMSHVFTSRNYERYVTLSIGIAVYPETGIDAKFLHKN
                     ADTAAYKAKTGGRNQFRMFGFDMHTATLEQLLLEDSLRAALARKEFKLYYQPIVNTNS
                     GKLTRVEALLRWDHPDLGIIAPDYFIHLAEETGLIFQIGEWVIREACSQFRAWNKGGI
                     LMPNVSVNVSAVQLRKMNLFSIVAGAIRENSMKPFELDLEITESFAMQNMELSLGFLE
                     DLGGLGVGLSIDDFGSGYSSLAYLKRLPIQRLKIDRSFIKDMPDDRDSNAIADAIVAM
                     AHSLELGVIAEGVESARQMEYLKGIHCDELQGFYFARLRRRTKLSSQNFPDRSERLWP
                     LHLDPLRPTLN"
     gene            6207..6668
                     /locus_tag="PLG78_04435"
     CDS             6207..6668
                     /locus_tag="PLG78_04435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNE22385.1"
                     /translation="MAIASGSLAPDFELIADNGVPWKLSSARGKKVLLVFYPGDDTLV
                     CTAQLCDYRDGIESFRGFQTDVVGISMNDKESHQKFKEKYDLPFPLLSDPDHKVAAQY
                     GCMSPLGTRRGVYLIDEKGIIRYGHVELMTLFKRSREELLKVLAQIPNREA"
     gene            6658..7248
                     /gene="yihA"
                     /locus_tag="PLG78_04440"
     CDS             6658..7248
                     /gene="yihA"
                     /locus_tag="PLG78_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNE22386.1"
                     /translation="MKPDYFRGVEYKGSYVKAEECPSPASIPVVAFTGRSNSGKSSLL
                     SSICDHQNLARTSKKAGKTVTLNYFLVPPGSSGGPGLYLVDLPGYGYATLSHAERERI
                     RVMVDEYLEATPFRLLVIVLDARRKPAQEEISIRQWCEAHGFRFVFALTKWDKLNNKE
                     RSQTLHTWKEFGAPHQPVSNESGYGISEMIKLVRGR"
     gene            complement(7226..8071)
                     /locus_tag="PLG78_04445"
     CDS             complement(7226..8071)
                     /locus_tag="PLG78_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE22387.1"
                     /translation="MKNTGLCLNSAYFGFYAHAGFVHGMVRGGFAPSIVTGCSSGAMI
                     GALYCAGIDMDEVVRLIQKIRKNDFWEGNALTQAAKIFARGVSRYSGLLTGRKIRALL
                     EPYLGGLKFSDLKIPLGVAVSNVTTGRRELCTEGSVLDSVMCSMAFPFLMEIQKMKGN
                     DYIDGGVVDHEPTKEMILDPSVRTIVVHMIETERETPPRSPIVRAFHASLSIIDHETR
                     DLKDRLALARKTKIVRAMTRTPHLGPDRTHYGETALHAGQKSAASILRSLRIPRSRAN
                     GRGLA"
     gene            complement(8068..9546)
                     /locus_tag="PLG78_04450"
     CDS             complement(8068..9546)
                     /locus_tag="PLG78_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22388.1"
                     /translation="MFFSVTYALYEAWRIRWIADDAYISFRYILSFISGKGLTFNEGE
                     RVEGYTNFLWTVLLIPFRALGLDLEVVSLVLGCFFFGMLLCAFAFCVRKALSIEFAAV
                     ALLALCAHRHLQIFATSGLETMLFTFLATTGVLLLALKHKAAATEEPERRLAIIAFFS
                     LALATLTRPDGAIIYAVCAGYLFVFEWRQNGPVSAARESVRNHLLSVGILFPVLVAKS
                     LYYGSIVPNTFYAKSASHPYLFQGIRYAALYFESYWPLGIALILATVFWRILPLIRLP
                     VLVVVAYLSFVVFVGGDFMFARFLIPITPLLFYCSVVLLSKIASSRARAVAFAAFVIA
                     IVFRLDPFGGSLSRDGITEENRLYSRSARALLRETALSLRPALIRANPRIAFVGAQAA
                     FAYYWYPLFAIESETGLTDKTIASQSIEQRGKVGHEKNADPEYLRSRHVHIWMRPPPP
                     GFSGPVLQMAGIPGDMGVIEEDPRIFSILTSDDRFVVDGGTH"
     gene            9530..10198
                     /locus_tag="PLG78_04455"
     CDS             9530..10198
                     /locus_tag="PLG78_04455"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE22389.1"
                     /translation="MTEKNIKSDMPIFLATRKDTGCMVAVKTMLEFCFKIATLLLTLF
                     YAAVRSIYQYRYNQKHKKRRVHFLRESFLVTLVAVCWWGSTGLYVFTNFLENGSFHLH
                     TIARIAGGCIMFASIVYFWRVHSFLGKHWSPVLEIEHEHTIIETGPFRTVRHPMYSAI
                     IVSILSQGLLTANLFVLIPSLVSFLFLCWIRIPAEEKMLEEEFGEEYAKYKSRTWIFF
                     PWVL"
     gene            complement(10193..>10433)
                     /locus_tag="PLG78_04460"
     CDS             complement(10193..>10433)
                     /locus_tag="PLG78_04460"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HNE22390.1"
                     /translation="GLRYGFGYCFLTDWHWSVLESQGVRNLPGSYVKYLFDLITGWDS
                     SPFWIDVATGVSFSIAISSSLYVNRDLFRFLRPTS"
BASE COUNT         2915 a         2690 c         2550 g         2278 t
ORIGIN      
        1 aaaaaccaac ttgaaagata ctcttctcta ttccgaatac cgttatacgc ttatgtgatg
       61 cacgctgcca aattcgattt gctttctgaa tgctttgtcc ggtgacaagc cggtccaacg
      121 aatcaacaag ctgcgaatga ctcctgcgtg gctgatgagc actgctcctt cctttcctgc
      181 gtcccgtaga gtctgtaaga attcagtgag gcgatcctcg acatccgcaa acgattcacc
      241 gcccggcgtt cgctgtgtga cataatcatt catccagaga tctagctctg ttcgaggtat
      301 gtcatcccag cgcaagcctt cccattcgcc aaaatgcatt tcagccaggc gcgcatccac
      361 tgcatggtcc ggatcaatca agtgcgccaa ttgtctgcat cgactcagat gactggaata
      421 caccggcaga gaaggaagac tttcgcgcag tgagttgccc agcacttcaa agccaggctt
      481 gagtgcgaca tcgagctggc cgtagcaaac gcctttgtcc actgctaccg gtggatgccg
      541 aactacataa aggtccatat tgccagagtg gctccataga aaacgacttc agtcacctgt
      601 tgcactgctc cgaggcaatc gccagtataa ccaccaatcc attttctcat gtagagcgcc
      661 ataagcaacg ccgttaccgc agaagcaacc gcgggaatca gcatatacaa tcttccgaaa
      721 tatgccagcg gaacaaggcc gaataacgtt gccgcgacca tatcgagacc atgagtcaga
      781 acgccgatgg gttttgtaac tgcggacaca tcacgtacat acggaagaaa tttcatgatc
      841 cacaccggaa acattctact gatagaatga cctgcgatca gaagagccgg aagcctcgcg
      901 gaattcaagt caatcatcac atgatacttt aacagaaaca atagaatcag tgcgatcgtt
      961 ccaaacgctc caatgcgcga gtccttcatg atctcaagaa ttttctcttt tgtccagccg
     1021 ccaccaaacc catcacagaa gtctgcgaag ccatcttcgt gaaaagcgcc tgtaaccaga
     1081 acaccggcaa tcatgcaaag aaccacagag atctcgcgcg aaaatagacg attcgcgagg
     1141 agaaaaacaa ccgcgcagat tccaccgacg atcgctccga ctaacggaaa gtaagcgacc
     1201 gatctgttca gcctttcttc agaatgatct atccaggaag ggcagggaat gcgcgtcaga
     1261 aacatcaccg cagtgagaaa tgtacggatc aactaacagc acctcggatt cttgaacctt
     1321 ctctcttcct ctctggccca ggtttccccc gcagataccg ccgcaagaga agaacgctct
     1381 acagatcgat cagaagaaag gcgcgctgct tcaaagcgat tctgtcccga cagatgacga
     1441 aatagctctg cgagaaaaaa tcccatcatc cagaatttcc atggaggcct ggcggcagct
     1501 tccgcagcaa tgacttcatt ggaaagccac acgggagaag tttcggtcag acttctggac
     1561 ccttttccaa acagcattcc cgcccagcga atgatagaaa gaatcaggat ggaacctacg
     1621 aggagaagaa acgcgcccgc aacgagagcg tctaactgca tgttaaagat gattcttccg
     1681 gtttcgccaa tcttttccgg cggaacctgt ccatgcgcca gtttgtcatt caaggaagca
     1741 gccatagaca gaaaaccgat cctcggatcc ggatgataaa tcttttccag gcatgcggtg
     1801 aacgtaactg cagtcagcca cgtcagcgga atcagagtca cccagagaaa tttgattttg
     1861 tccatcttga tgagaatggt tgtggccaga cacagggcga ccagagacag gagctggttt
     1921 gcaatgccga ataacggcca gagactattg attcctccca gaggatccgt cactcctgaa
     1981 taaagaaaat atccccagcc tgacacaaga agcacacttc ccagaacgtt ggcgccgtac
     2041 gaatgagtat ttccaagcgg cttgtaaatc tgcccgagca acgcctgcag caagaatcgg
     2101 ccaacccgag tccccgcatc caccgttgta agaatgaaca acgcttcaaa catgattgca
     2161 aaatgatacc agagtgcaag gagtgcgggg cccgtcaggc gagcaaagat atgcgccatc
     2221 ccgactgcca gagtgggtgc gcctcctgct cgaccaaaca ttgtcttttc acccagttct
     2281 gcggccagca cctgcatctc ctgaagagtg agtggaaatc cccagcctga aatcgtctgc
     2341 acgatcactt ctggtgtctt gcctacagca gatgggcttg cgttcatcgc aaagtactga
     2401 ccaggggaaa gagtacaggc cgcgatcaaa gccataatcg caacaaatga ctcaaggagc
     2461 atacttccgt aaccaaccga acgcgcatga ctttcgcgag taatcagctt tggcgttgtt
     2521 cctgatgata tgagcgaatg aaaaccggag attgccccgc atgcaatggt aataaagcag
     2581 aaaggaaaca cgtctcccga aaacaccggg cccgtgccat cgacgaatcg ggtcaggggt
     2641 ggaaatgtaa gcggaggtaa aaccagaaat atgcccagag caagagcgcc tatcgttcca
     2701 agcttcatga aagtactcag atagtcgcga ggagcaagaa ggagccacac aggcaaaatg
     2761 gatgcggcca ggccgtatgc aatgatcatc cacgcaagag cgggctcaga aaacgtaaga
     2821 acgtcaccga gtgcgggatc ctgttgaaag aactttcccg cccagacgga aataagaaga
     2881 agaatcactc ccactgtagt gatctcgagt acctttcctg gtcgaatgaa gcgaaggtaa
     2941 actcccatga acagagcgat tggaatggtg gccgcaatcg taaacgttcc ccagggacta
     3001 tgcgcgagag ctttgactac aatcaagccg agtaccgcga caagaattgt aatgatagaa
     3061 acgatcgcta ccatggcgac gatccccgca ggtttgctga tctcttcttg aaccatctct
     3121 cccagagact ttccgtttct tcgcatggaa gaaaatagaa tcataaaatc ctgcactgct
     3181 cctccgagaa caacgcctat gagaatccag agcgttccag gaagataacc aaactgcgcg
     3241 gcgagcacag gacccacgag gggaccaggg ccgctaattg cggcgaaatg atgcccgaat
     3301 acgatccact tattggtgcg aacaaagtcg cgcccgtctt cgtgcacttc gcacggtgtc
     3361 gcacggcgat catcgagagt gagaatcttc gccgcgatcc atttggaata aaagcgatat
     3421 gcggtggcgt aagtgcagag cgcggcagtc aaaatccagg ccgcgttcaa gccttcgcca
     3481 cggtgcatgg ccaccataat gtaaccaaga gcgcccacca gtgcgacgca aatccaggcg
     3541 ataacggcga gaggtttctt catcattcga aaattgattt gcactgcatg gagagcaagt
     3601 gattcttatc agacgtcatg gctatctcaa gagtgagaat ctacgaagtt ggcccccggg
     3661 acggccttca aaacgaggca cgaattgtaa gtgcccggga caagatagaa ttcattcgcc
     3721 ttctttctct ctcaggcctg accaacatcg aagcgacggc cttcgtgcga tccgtgtcac
     3781 agatggcaga tgcacaggaa gtgtacactt cagtccgaga ttttggacca agaatgagtg
     3841 ctctggtccc gaatcgcaaa ggaatggaga acgcactggc ctccgggctc cgcgaagcgg
     3901 caattttcac cgcagcatca gaaactttca caagaaagaa catcaacagt tcaattgaag
     3961 agagctttag ccgatttaaa gaggtaatgc aactagccgc ggaccatgcc attcctgtgc
     4021 gcggatacgt gagcactgtg gtcgaatgcc cttacgaagg taggatcgcc ccgcaaaaag
     4081 tggcggaaat ttgtgacaga ttgatccagg caggcgtcta cgaaatctca ctgggagaga
     4141 ccatcggagt cgctgtgccg gacgacatct cgagacttct cgatgaggtc acaaaaaagg
     4201 tgccgccgga gaagctggcg ggtcactatc atgataccag gggcacggcg ctctccaatg
     4261 tcttccgctc tctcgactac ggactgagtg tttttgattc gagcgccggt ggcctgggtg
     4321 gatgccccta tgcacctgga gcgtcgggga atctggcaac agaagatttg ctgtatgctc
     4381 tgcatcgcag taagattgaa acaggcgtag acctgggcaa aatcgcagac gcaagtgagt
     4441 tcatccgtca tgcgattgca aaggagcccg cttctcgggt cttcgcggct attagaaaat
     4501 gaaagttgtg aaatcatgga tcatctgaat ctaagcgaaa agctccggtt gtatgcggaa
     4561 gcatttgaga aagcgctcga atgcatgctg atcgcggatg aaaaccgcag ggtcattgcc
     4621 gtaaatccag agttcgaacg agtcacgggc tatatgctct cggaagttct gggcaagcgt
     4681 ccgggttttc tggattccgg aaatcactca ctcagattct atagaggaat ctggagaagt
     4741 gtgcgagaca ctggttcctg gcagggagaa atccagagca agcgaaagaa tggagaactc
     4801 tattctgaat ggctcagcat cagccagctt catgaaaacg gacacattta ctaccttgca
     4861 gtattcagcg atatcaccca tagaaaagat tacgaaagaa cgttatacgc tctagcaaac
     4921 tatgatacat taactgggct tcccaatcgc aatttgttct cagagattct ggaaaaagaa
     4981 gtagcagagg ccggacgcaa aaatgaaaaa gtcgggcttt tgatcataga tgtagaccga
     5041 ttcaaatcgg tcgtgatgac tctgggccac actacggcag atgaactcct gatcgacatt
     5101 gtccatagaa tccgaacctg cctcagacga cacgatgttc tggcgcgtct gggtggagac
     5161 gagtttgcag tcattctccc aggtcttacg gaaagtcgag aagcttcgag cattgccaga
     5221 gaaatcctgg ctgccatgtc ccatgtattt acctcacgca attatgaacg ttatgtgaca
     5281 ttgagcattg gaatcgctgt ctatccggag acaggcattg atgccaaatt cctgcacaaa
     5341 aacgcggata ctgcggctta caaagcaaag acaggaggtc gcaatcagtt tcgcatgttt
     5401 ggttttgaca tgcatacagc cactctggaa caactgttat tggaagacag ccttcgcgcc
     5461 gctctggcga gaaaagaatt taagctctat tatcaaccca tcgtaaatac gaattccgga
     5521 aaactgaccc gggtagaggc acttctcaga tgggatcatc ccgacctggg aatcatcgca
     5581 ccagattatt tcatacacct cgcggaagaa actggattga ttttccagat tggggaatgg
     5641 gtgattcgcg aagcatgttc acagtttcgc gcctggaaca aaggcggaat attgatgccc
     5701 aatgtttcag tcaacgtctc cgccgttcaa ctgagaaaga tgaatctgtt ctccattgta
     5761 gcaggcgcga ttcgcgaaaa ctcaatgaag ccatttgaac tggaccttga aatcacagaa
     5821 agcttcgcaa tgcagaacat ggagctttca cttggatttc tggaagatct cggtgggctc
     5881 ggagttggtc tgtccattga tgacttcggg tcgggctact cttccctggc ttatttgaaa
     5941 agactcccca tccagcgcct gaaaattgac cgctcgttca tcaaagacat gccggatgat
     6001 agagacagca atgcaatcgc agatgcaatc gttgccatgg cgcatagcct cgaattgggt
     6061 gtgattgcag aaggcgtgga gtccgccaga caaatggaat acctcaaagg aattcattgc
     6121 gacgaactac aaggattcta cttcgcacgg cttcgccgcc ggacaaagtt gagttctcaa
     6181 aattttcctg atcgatcgga gagactatgg ccattgcatc tggatccctt gcgcccgact
     6241 ttgaactaat cgcagacaac ggagtgccct ggaaactttc aagcgcacga ggaaaaaaag
     6301 tgctgctcgt gttttatcct ggagatgaca cgctcgtttg caccgcacag ctctgcgact
     6361 atcgcgacgg gatcgaatcc tttcgcgggt ttcagacaga tgtggttgga atcagcatga
     6421 atgataaaga atcgcatcaa aaattcaaag agaagtacga tcttcccttc cctcttctgt
     6481 ccgatcctga tcacaaagtg gccgcgcagt acggatgcat gtctcctctt ggaaccagaa
     6541 gaggagtgta cttgatagat gaaaaaggaa tcattcgata cggacacgtc gaactgatga
     6601 ctctctttaa acgttcacgc gaagaattgc tcaaagtctt agctcagatt ccaaaccgtg
     6661 aagcctgatt actttcgcgg agtagagtac aaaggatcct acgtgaaagc ggaagaatgt
     6721 ccatctcccg cctccattcc agtcgtcgct ttcactggca gatccaattc aggcaaatcg
     6781 tctctgctct cctccatctg cgatcatcag aatctggctc gaacatccaa gaaagccggg
     6841 aaaacagtca ctctgaatta ttttctggtt ccacctggaa gctccggtgg acctggtctg
     6901 tacctcgtgg atcttcctgg ttatggttac gcaactcttt cacatgcgga gcgcgagcga
     6961 attcgagtca tggtagacga atacctggaa gcaactccat ttcgtttgct tgtcattgtg
     7021 ctcgatgcga gaagaaaacc cgcccaggaa gagatttcca tccgccagtg gtgcgaagct
     7081 cacggatttc gctttgtatt tgcgcttaca aaatgggaca aactcaacaa caaagagcga
     7141 tcgcaaactc ttcatacatg gaaagaattc ggagctcctc atcagcccgt atccaacgaa
     7201 tctggctacg gaatatctga aatgatcaag ctagtccgcg gccgttagcc cgcgagcgag
     7261 ggattctcaa agagcgaaga atgctagccg cactcttttg tccggcatgc aaagctgttt
     7321 caccatagtg cgtgcgatcc ggacccagat ggggtgtgcg cgtcatggct cgaacaatct
     7381 ttgtcttgcg ggccagagca aggcgatctt tcagatcacg cgtctcatga tcaatgatcg
     7441 ataagctggc gtggaatgcc cgcacgatcg ggctacgcgg aggagtctcg cgctctgttt
     7501 caatcatatg caccacaatc gtgcgcacgg atggatccag gatcatctct ttggttggct
     7561 catgatccac gactccgccg tcgatgtagt cgtttccttt catcttttga atttccatca
     7621 gaaacggaaa tgccatcgaa cacatcacac tgtcgagcac gctgccttcc gtgcaaagct
     7681 ctcgccttcc tgtggttacg ttcgataccg caacgcccag tggaattttc aaatccgaaa
     7741 attttagtcc accaagatat ggctcaagaa gtgctcgaat cttccttcct gtgagcaatc
     7801 ccgaataacg ggaaactccg cgcgcgaaga tctttgccgc ctgggtgaga gcgtttcctt
     7861 cccagaaatc gttttttcga atcttctgaa tcaagcgcac cacttcatcc atgtcgattc
     7921 ccgcacaata gagcgcgcca atcatcgctc cggaggaaca accggtgacg atcgaaggcg
     7981 caaagccacc gcgcaccata ccatgcacga atccagcgtg tgcatagaag ccaaaatagg
     8041 ctgaattcag gcaaagccct gtgttcttca atgtgtgcct ccatcgacaa cgaaccgatc
     8101 atcggatgtg agaattgaga aaatacgcgg gtcttcttct ataacaccca tatctccggg
     8161 gatccccgcc atctgcaata cagggccgga aaatccggga ggtggtggcc tcatccagat
     8221 atgaacatga cgcgaacgga ggtactccgg gtccgcattt ttctcatgac ccaccttgcc
     8281 tctttgttca attgactgcg aagcaatcgt tttgtccgtg aggccggttt ccgattcaat
     8341 ggcgaataac ggataccagt agtaagcaaa cgcagcctgc gctcccacaa acgctattct
     8401 aggattcgca cgaatcaaag caggacgcag agagagagca gtttcccgta gcagcgcccg
     8461 agcgcttcta gaatacaatc gattctcttc tgtaatcccg tcgcgcgaca gactgccgcc
     8521 gaacggatcc aacctgaata caatcgcaat gacaaaagca gcaaaagcaa ccgccctggc
     8581 tctggatgag gcgattttag atagtagaac cacggagcag taaaacaaaa gtggagtgat
     8641 cggaatcaga aacctcgcaa acataaaatc gccacctaca aacaccacaa aggaaagata
     8701 ggcgaccaca accagaacag gcagtcgaat caaaggcaaa atgcgccaaa atacggtggc
     8761 cagaatcaat gcaatgccca gaggccagta ggactcaaag tacaatgccg cataacggat
     8821 tccttgaaaa agatacggat gagatgctga cttggcataa aaggtattgg gtacaatgga
     8881 tccgtagtac aaactttttg caaccagaac cggaaaaagg attccaacgg aaagaagatg
     8941 attgcgcaca gattctcgtg ctgcagacac cggtccgttc tggcgccatt caaagacaaa
     9001 caaatagccg gcacacacag cgtaaatgat cgctccgtca ggacgagtta gagtggccag
     9061 agccagggaa aaaaatgcaa tgatggcaag tcgtctttcc ggctcctctg tagcggcagc
     9121 tttgtgtttc agtgcgagaa gcaacacgcc cgtagttgca agaaatgtaa agagcatggt
     9181 ctccaggcct gaagtggcga agatttgcaa atgcctgtgt gcgcagagag ccagaagggc
     9241 aacggccgca aattcgatag aaagagcctt tcgcacgcag aaggcgaacg cacagaggag
     9301 cattccaaag aagaaacatc cgagaaccag agacacgacc tccagatcga gacccaatgc
     9361 tcgaaaagga atcagcaaaa ccgtccacag gaagttggta tacccctcca cgcgttcgcc
     9421 ttcgttgaac gttagacctt tcccggaaat aaaggaaaga atgtaacgaa atgaaatgta
     9481 ggcatcatcc gcaatccagc ggatgcgcca ggcttcatat agagcatacg tgactgagaa
     9541 aaacatcaaa agcgatatgc cgatttttct ggccacgcga aaggacaccg ggtgcatggt
     9601 tgcggtcaag acaatgcttg agttctgctt caaaatcgca acgctccttc tcaccctctt
     9661 ttatgctgcg gttcgcagca tctatcaata ccgctacaac cagaaacaca agaagcgccg
     9721 cgtgcacttc ttgcgcgaat cctttctggt gactctggtt gcggtgtgct ggtggggatc
     9781 cactggatta tatgtattta ccaactttct agagaacggt tcatttcacc tccacaccat
     9841 tgcgagaatc gccgggggat gcatcatgtt cgccagtatt gtgtatttct ggagagtgca
     9901 ttcttttctg ggaaaacatt ggtcccccgt gctcgaaatc gaacacgagc acacaatcat
     9961 cgagaccgga ccgttcagga cagtccgtca tcccatgtac agtgccatta ttgtttcgat
    10021 tctgtcgcaa ggactgctca ctgcaaactt gtttgtgctg attccgtctc ttgtgtcttt
    10081 cctctttcta tgctggattc gcataccggc agaagagaaa atgctggagg aagaatttgg
    10141 agaagagtat gcgaaataca aatcgcgcac ctggatcttc tttccgtggg ttctatgacg
    10201 taggacgcaa gaatctgaac aaatccctat tcacatacag tgaactcgag atcgcaatgg
    10261 aaaagctgac tcctgtagcc acgtcgatcc agaagggact tgagtcccat ccggtgatca
    10321 aatcaaataa atacttcacg taagaaccgg gcaaatttcg cactccctgt gactcaagga
    10381 cggaccagtg ccagtccgtc agaaaacaat aaccaaaccc gtagcgaagt ccc
//
LOCUS       DAOQJL010000064        10425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3316_length_10425_cov_4.145516, whole genome shotgun sequence.
ACCESSION   DAOQJL010000064 DAOQJL010000000
VERSION     DAOQJL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_10425_cov_4.145516"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLG78_04465"
     CDS             complement(<1..430)
                     /locus_tag="PLG78_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22391.1"
                     /translation="MKKIGLILIAVYHGLLFVVCSMNLMANNFVLKASRFYDEQLVSS
                     DLMSRWILLAPLALPLWMRALRSAGVTGFRITPAFLILAFCSICLNYPFALTGAQGVD
                     FWDILRAAVRDGLTQPSAFCISVLSLVGLIEFRNVEEKRLQ"
     gene            complement(440..2479)
                     /locus_tag="PLG78_04470"
     CDS             complement(440..2479)
                     /locus_tag="PLG78_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE22392.1"
                     /translation="MQKLFYPLLGLLFVLSTLLSLAFGSLDVSLLREGGAFLVMVVLP
                     FWVYHRRRFKTLADHVFFWASVLTITHSFLNGITYLADTPVPRLKLYLKTFFLASILL
                     WGIAWFRGASRSLLVALCILFSANVLLSWYFLGFTLRSTVLAAYLIGGLVYALEEVET
                     VRLDYRDAAMVLLFGAGLLSTIFSWDRSSAFFGYTHLGLALPLFFLGRHLSKTADVPE
                     FLRNCVFAFSGVMIMFAVFLLVTLYTSGQPLTDKMEKIAGYHVNGLGASIALFLPLCI
                     YVGVNDSNPWVRIWSRSCAIISAGMLLIISSRGSMVGVIISLLLLVIQVRRVRLSGWS
                     ARAIVILLIGAGAVVLYFVEPLRQYVQALGSPESMKIRLMIWRLHIANVLHYSPWIGF
                     GPDTFIINAVIPPADPELSGALKPFMRGFGPIVHAHNVFVQLLQDFGLLGILFSVILG
                     VSVAGVAWRFLFGADAPGLRSYLPYGFAALTALFVQGLFDYTLSDPMTYYPAVMILGM
                     ILQVAPGKHALEMSLHKITSIVFGAGIVLFVVLFWMGWNRTLYISETSVFRGQTLSDR
                     FGNVTFLGISVFTDEQIRAFQFRDRLLLPGNTDHRIEQLAGEFYFQRFKRQGYTDGLT
                     VARSRFERCLAIHPYSALCAWRLSTIHHELGSKTEEERYLDLASIHDPYKLVIDQ"
     gene            2737..3009
                     /locus_tag="PLG78_04475"
     CDS             2737..3009
                     /locus_tag="PLG78_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22393.1"
                     /translation="MKKYVLSLAISSLFSTAVMASANLYNSDSQSYRLLLDSSESCFS
                     GTHTSIESNTRTSVSSGWACLGEEKPAVKLEDGKSYVIRNGVIQEE"
     assembly_gap    3340..3439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3440..3613
                     /locus_tag="PLG78_04480"
     CDS             <3440..3613
                     /locus_tag="PLG78_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNE22394.1"
                     /translation="RAAIAYCETVKDYGSRELLEEILESEEEHVDFLETQLDLVERVG
                     IQNYSQSQMETPS"
     gene            3615..4775
                     /locus_tag="PLG78_04485"
     CDS             3615..4775
                     /locus_tag="PLG78_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE22395.1"
                     /translation="MRIAVALPVLLTFLSCVSPRLVETQPAYKVEDIPVVFIHGMLGS
                     VLVDSSDEIQYLTAGEALGLRTPDLRLPTLWDGDKQPLDDLRPAGPLMRVGLIRHVID
                     QAVYAPWIEFASHIPGRPFYAFSYDWRRDGNESADQFEKFIETISKRHGKKVQIIAHS
                     MGGIITLVVQNRRPDLIDRVVFAGVPFRGGLGYLDNLYLGTPIGANKNLLSPEVIFSH
                     PGVYSFYPAGQSFENKTLAEDESGKALDIDFLDIDAWQKNGFGPFAPQNKSWGGEKHK
                     AFLAGTLARIRSFRKKMQPDKSKSYPRALVITSDTYPTLARIRRIPPASGESVPRWDF
                     DSAAKGQGDGSVLRADAIPPEPIQSEVYLSKYGHSYLLNDPNAQDAIVKFLK"
     gene            complement(4767..6077)
                     /locus_tag="PLG78_04490"
     CDS             complement(4767..6077)
                     /locus_tag="PLG78_04490"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE22396.1"
                     /translation="MRTNIVHIGAGELQYEIRQIVEIGHNVMRLSGKPIAWENIGDPV
                     QKGEKLPDWMKDIIAQAVRDDSTYAYSPTRGLLETREFLAERLNREKGVKIHPDDIIF
                     FNGLGDAVNKVYGMLRREARVIGPTPAYSTHSSAEAAHAGYPPITYRLDPARGWMPDL
                     EELRNKVKYNESIAGIMIINPDNPTGAVFPRDVLEEMVEIARQYDLFLVGDEIYVNMV
                     FNGKKCTNLSEVIREVPGISLKGISKEFPWPGGRCGWMEVYNQASNPMFQKYVKSVLD
                     AKMLEVCSTTLPQVVIPRIMGNPEYVNWQKQRNAFFEKRSKRIAEILSGIPEIIVNPP
                     DGAFYVSIVFKKELNNRMKLNIPNAAVREYIDGICSGNMEPDRRFVYHLLASRNICVV
                     PLTSFVCTLQGFRCTLLERDDAKFEEIYHGVRDAILEFLNSASL"
     gene            complement(6103..6906)
                     /locus_tag="PLG78_04495"
     CDS             complement(6103..6906)
                     /locus_tag="PLG78_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22397.1"
                     /translation="MMRACLLVSVALCAHSLWAQADPALPEPQSPSPTPYVSIHAGPA
                     VSASGSIVASSWAGLRLLELLVNSNVLYAPRISPALRYTGPIYKVPPLELSSRQYGFL
                     YERRMDHWFFGGALSYTDIKARLQIPETFVVAPNLGLNVPLVFRVTPEIHGRMLLASA
                     LQAEGSAAYHFWPEEVLDPYVRVFAGVGKGFVAHPLGGPYLHEIHAGTAVGLRWNRES
                     LFFGSEVNVIGHWVNSGPSSFIDRTNVLVNPGNGSIYIVRLLLSAGMRF"
     gene            complement(7009..7755)
                     /locus_tag="PLG78_04500"
     CDS             complement(7009..7755)
                     /locus_tag="PLG78_04500"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNE22398.1"
                     /translation="MSQSLEYLELQLYAGHLKGACSYLPEKESNLLFLEATGLGAGYR
                     LLLDRGYRRHGFHVYRPDCANCQECKILRLPVATFAPTRSQKRVWKKGQSVFRVETGP
                     PSYSREKEQLYCKYLACQHGSCDDMDPCRYSDFFVSSFLATTEELRLYAGDRLVGLGI
                     FDVVGDALSSVYFFFDPDYSRYSPGTFSILAEIDLCKARGLSHYYPGYFISACHAMNY
                     KARFGPAEVKLPAQEKWGPCVKSCLPWVDE"
     gene            7797..8879
                     /gene="serC"
                     /locus_tag="PLG78_04505"
     CDS             7797..8879
                     /gene="serC"
                     /locus_tag="PLG78_04505"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNE22399.1"
                     /translation="MRRVYNFSPGPAALPLPVLETIRDELLDYQNTGMSIMEMSHRGK
                     VFEGLLDDINQRLRKLASIPDRFDILYMTGGASAQFAFVPMNLSGKGRKAGYVNTGVW
                     SEKAIEQANLLGIQVFDAGSSKDKNHSYIPEVKTTSGLDYLHVTSNNTIYGTQFQKLP
                     SPGDTKLVIDMSSDFLSRPIDWSNIGMVYAGAQKNAGPSGVTIVVVDREYYGRESAET
                     PTVFRYSTYAKNNSLYNTPPTFQMYVFGLVVSWIEKQGGLEEMHRYNQHKAELIYNVM
                     DEFPKFYTGHAVKNARSLMNITFRLPSEDLEGKFLKAAEERKLMGLKGHRSVGGLRAS
                     TYNAVGEEACQVLSVLMREFARSNGG"
     gene            8881..9087
                     /locus_tag="PLG78_04510"
     CDS             8881..9087
                     /locus_tag="PLG78_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22400.1"
                     /translation="MLWLRLGDNELVQLDHVVSIKKQEPQAIELTFLEPNGKRILTFP
                     DPAERNRAFDRLLENLVKLRMALE"
     gene            complement(9141..10169)
                     /locus_tag="PLG78_04515"
     CDS             complement(9141..10169)
                     /locus_tag="PLG78_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE22401.1"
                     /translation="MNGAYLLLGAMIGSAMMPRYPRFVYVTNFSSATVSMYTINQTTG
                     VLSANGTIAAGTQPRDIVVDPSGRFVYAANNGSNSVSMYSINQTTGSLTSIGTIAAGS
                     STQGLAVDPGGNFIYAVNNASNNVSMFTVNQATGMLSPNGTIAAGTGCLGIAVDPTGK
                     FAYVANNTSNNVSMYTINAVTGVLSANGTIGAGTGPMGVVADPTGKFAYVTNNASNNV
                     SMYTINPGSGVLSANGTIAAGTGPIGIASHSSGQFAFAANFTSNNLSMYTINTVTGVL
                     TANSTVAAGTGAQAIAVDPSGKFAYSGNGSANNVSMYSVNTSTGNLTSNGTIPAGSNA
                     IGIAIAYF"
BASE COUNT         2834 a         2678 c         2564 g         2249 t
ORIGIN      
        1 tctgaagacg cttttcttcc acattcctga attcgatgag cccgaccaga gaaagaactg
       61 aaatgcaaaa agcggaaggt tgcgtcaggc catctcgcac agcagcgcga agtatatccc
      121 agaaatcgac gccctgggcc ccggtaagag caaatggata gttcaagcaa atggaacaaa
      181 aggcaagaat taagaaagct ggcgtgattc gaaagccagt cacccctgcc gatcgcaatg
      241 cacgcatcca gagaggaagt gcaagcggtg ccagtagaat ccagcgcgac atcaaatcac
      301 tggagactag ctgttcgtcg taaaatcgac ttgctttgag aacaaagttg ttggccatga
      361 gattcatgga acaaacaaca aaaagcaaac cgtgatacac cgcgattagg atcagtccta
      421 ttttcttcat cgcgtctgcc tactgatcga ttacaagctt gtaaggatca tgaatgcttg
      481 caagatccag ataacgttcc tcttccgttt tacttcccag ttcatggtga atcgtggaaa
      541 gtcgccatgc gcaaagcgca gagtaaggat gaatggcaag gcatcgctca aagcgagaac
      601 gcgcaacggt cagaccatcc gtgtaacctt gtctcttgaa ccgctgaaaa tagaattcgc
      661 ctgccagctg ttcgattctg tgatccgtat ttccaggcaa aagcaaacga tcacgaaatt
      721 gaaaagcgcg tatctgttcg tccgtgaaca cgctgatgcc caggaaagtc acgtttccaa
      781 atcgatccga tagcgtttgt ccgcgaaaca cgctggtctc ggaaatatat agagtacgat
      841 tccatcccat ccagaacaag accacaaaga gcacgatgcc agcaccaaac acgatagacg
      901 ttatcttatg aagggacatt tccagagcat gcttgcccgg agctacctgg agaatcattc
      961 caagaatcat gacggcagga taataagtca tcggatcact gagagtgtaa tcgaacaatc
     1021 cctgcacaaa caaagctgtg agagcagcaa atccgtacgg cagataggag cgaaggcctg
     1081 gcgcatccgc gccaaatagg aagcgccatg cgacaccggc aacagagacg ccaagaatca
     1141 cagagaacag aatccccagc aaaccaaaat cctgaagaag ttgaacgaat acattgtgcg
     1201 cgtgcacaat cggaccgaac cctctcatga aaggtttcaa ggctccactc aattcggggt
     1261 cagcgggtgg gatcaccgca ttgatgatga atgtgtcagg tccaaaacca atccacggag
     1321 agtagtgcaa aacattcgca atatgcagac gccagatcat gaggcgaatc ttcatggact
     1381 cagggctacc gagggcttgc acatattggc gcaacggctc gacgaaatat agaacaacag
     1441 caccagcacc gatcaagaga attacaatgg ctcgagcaga ccatcctgac agccgaactc
     1501 tacgaacctg aatgaccagc aaaagcaaag agatgatgac tcccaccatc gatccccgtg
     1561 aggaaatgat aagtagcatc ccggccgata tgatcgcaca gctacgactc cagatacgaa
     1621 cccatggatt cgaatcgttc acgccgacat agatacaaag gggaaggaac aacgcgatcg
     1681 atgcgcccag tccattgacg tgatagccgg caattttctc catcttgtcc gtaagaggct
     1741 ggccactggt gtacagcgta acaagcaaga acacggcaaa catgatcatc acgccactga
     1801 acgcaaatac gcaattgcgc aggaattcag ggacatctgc cgttttagat aaatgccgac
     1861 cgagaaagaa gagtggcagc gcgaggccca ggtgagtgta accaaaaaac gcactgctcc
     1921 gatcccacga aaaaatagta gaaagcaagc ccgcgccgaa cagcaacacc atggcggcgt
     1981 cacggtaatc caggcgcacc gtctcaactt cttccaatgc atacaccagg ccaccgatca
     2041 aatatgcagc cagaaccgtg ctgcggagtg tgaaacccag gaagtaccag gaaagaagta
     2101 cgtttgcaga aaataaaata caaagtgcaa cgagcaagct tcgagacgct ccgcgaaacc
     2161 aggcgatgcc ccacaataga atcgaagcca gaaagaaagt tttcaaataa agcttcaacc
     2221 tgggaacggg agtgtccgcg agataggtaa tgccgttcag aaacgaatga gtgattgtca
     2281 aaacagaagc ccagaagaac acgtggtctg cgagcgtctt gaacctgcga cgatggtaaa
     2341 cccagaaggg caacaccacc atgaccagaa atgcaccccc ttcgcgcaaa agagaaacgt
     2401 cgagagatcc aaaagccaga gacaagagcg tgctcaaaac aaacaggagg ccgagtagtg
     2461 gataaaacaa cttttgcatg caagccgggc taacgcgtgg cgcttcttcg aaaaaataag
     2521 cccttctcag gctttgacca gcatccggtc acgaccttca aagtaaacca agtttggatg
     2581 cagactttcc ggtggctctt caagcacatc agactttgaa gaaatgactt gagccaattg
     2641 aatcgctcat tgcctatgag tctcacgaca tgacgcatac ggttcgacaa gcgatccggg
     2701 tatgattcgg taacctcaaa ggtaggagat aacttcatga aaaaatacgt gctctctctg
     2761 gccatcagca gcctcttttc cactgcagtg atggcatcgg ccaatctgta taactcagat
     2821 tcccagagct acagactgct cctcgattca agcgaatcct gtttttccgg cactcacacc
     2881 agcatcgaaa gcaacacaag aacgtccgtt tcttccggtt gggcctgcct cggtgaagag
     2941 aagccagctg tgaagctcga agacggcaaa tcctatgtga tccgcaacgg agtcattcag
     3001 gaggaatagc ctggccattt cgggcgtttc cccgggtctg cccctgggga tggagcagtt
     3061 ttccgcttgc ctgagactgc gtctcaattt tatttggccg catgtatgtc tgtatctgca
     3121 aaggggtcac agatagccag atccgccggg gagtgcaaga ccggcgctgg cacaatctac
     3181 atgatcttca gaacgaactg caagtaggga cagcctgcgg caagtgcatc cgggaagcca
     3241 ggtccgtaat ggagaaagct caaaaggatt gcgcgatggg ctccgtgcga ggctttgaac
     3301 cagagccggc ataaggtagc cgaaatttcg gcaggagaan nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnna gagcggccat cgcctattgc gagacggtca aggattacgg
     3481 atcacgagag ctcctggaag aaatcctgga aagcgaagaa gagcacgtgg acttcctcga
     3541 aacgcagctg gacctggtgg aaagagttgg aatccagaac tacagccagt ctcaaatgga
     3601 aaccccgagc taacttgcga atcgcagttg ccctacccgt tcttctcact tttctctcct
     3661 gcgtttctcc ccgactggta gagacacagc cagcctacaa ggtagaagac atacccgtgg
     3721 tattcataca cggaatgctt ggatccgtgc tggttgattc ttccgatgaa atccaatacc
     3781 tgaccgcagg agaagctcta gggcttcgta cgccggattt acgtcttccc actctgtggg
     3841 acggcgacaa acaaccgtta gacgatctga gacctgccgg tccgctcatg cgcgtaggtt
     3901 tgattcgtca tgtgatcgat caggcggtct atgcgccctg gattgagttt gcttctcaca
     3961 ttcccggtcg tccattttat gctttttcct acgattggag aagagatggc aatgaatccg
     4021 cagatcagtt cgagaaattc atagaaacca tcagcaaacg acacggaaaa aaagtgcaga
     4081 tcattgcgca cagcatgggc ggaatcatca ctcttgtagt tcaaaatcga agaccggatc
     4141 tgattgatcg cgtcgtattt gcaggagtgc ctttccgggg aggactcggg tatctggaca
     4201 atctgtatct gggcactccg atcggcgcga ataaaaactt gctttcaccg gaagtcattt
     4261 tctcccatcc gggggtgtac tcgttttacc ccgccggcca gagcttcgaa aacaagacgc
     4321 tcgcggagga cgaatcaggc aaagcactcg atatcgattt tctggacata gatgcatggc
     4381 agaaaaacgg ctttggccca tttgctcccc agaacaaatc ctggggagga gaaaagcata
     4441 aggcattttt ggcaggaaca ctggcccgca ttcggtcttt cagaaaaaag atgcagccag
     4501 ataaatcaaa gtcttacccg cgtgctcttg taataaccag cgatacctat cccactctgg
     4561 ccagaatccg cagaatccct ccagcctctg gcgaatccgt tcccagatgg gattttgact
     4621 ccgcggcaaa aggccaggga gacggatctg tcctgcgggc agatgccatt ccgcctgagc
     4681 ctatccagag cgaagtctac ctttcaaagt acgggcattc gtatctgctc aatgacccga
     4741 acgcacagga cgcgattgta aagtttctaa agtgaagcgc tgtttagaaa ttcgagaatt
     4801 gcgtccctca ctccgtggta gatctcttcg aatttagcat cgtccctttc gagaagagtg
     4861 cagcggaatc cttgcaaagt acatacaaag ctggtgagag gcactacgca aatatttcga
     4921 gacgcaagca aatggtacac gaaacgacga tctggttcca tattcccgga gcatatacca
     4981 tcaatgtatt ctcgaaccgc agcatttgga atgttgagct tcatgcgatt attgagttct
     5041 ttcttgaaaa caatgctcac atagaaagca ccgtccggag gatttacaat gatctccgga
     5101 attccagaca gaatctcagc aatgcgtttg ctcctttttt caaagaatgc atttctttgc
     5161 ttctgccagt tcacgtactc tggattcccc atgatccttg gaatcactac ctgcggaaga
     5221 gttgtggaac atacttccag catctttgcg tccagaactg acttcacata cttctggaac
     5281 attgggttgc ttgcctggtt gtatacttcc atccatccgc aacgtccgcc gggccaggga
     5341 aactctttgg aaatcccttt caaggaaatg cctggtactt cacgaatcac ttctgaaaga
     5401 ttcgtacatt tcttcccgtt gaagaccatg tttacgtaga tttcatctcc gaccaggaac
     5461 agatcgtact gacgcgcaat ctccaccatt tcttccagaa catctcgagg aaacactgct
     5521 cccgtaggat tgtccgggtt gatgatcatg atgcctgcaa tgctttcgtt gtacttcacc
     5581 ttgttgcgaa gttcttccag gtcaggcatc catccgcgag caggatcgag gcgatacgta
     5641 atcggaggat aaccagcatg tgccgcttca gcagaagaat gagtggaata ggcaggagta
     5701 ggcccgatca cgcgtgcttc gcgacgaagc attccataga ccttgttgac tgcatctccc
     5761 agcccattaa agaaaatgat atcgtctgga tgaatcttga ctcccttttc acgattgagt
     5821 ctttctgcga gaaactcgcg cgtttctaaa agaccacggg tgggactgta cgcgtaagtg
     5881 ctatcgtcgc gaaccgcctg ggcgatgata tctttcatcc agtccgggag cttttctccc
     5941 ttctgaactg gatcaccgat gttctcccac gcgatcggct tgcctgaaag gcgcataacg
     6001 ttgtggccga tctcaacgat ctgcctgatt tcatactgga gctcacctgc tccaatatgt
     6061 acgatattcg ttctcatgac attcctggcc ctctccggcc gactagaagc gcatgcccgc
     6121 gctcaaaaga agacggacaa tgtagatgct tccattgcca ggattcacaa ggacgttcgt
     6181 gcggtcaatg aagctcgaag ggcctgagtt cacccagtgc ccgattacgt tgacttcact
     6241 cccaaaaaac agactttctc tgttccatct cagacccact gcagtcccgg cgtgaatttc
     6301 gtgaagatag ggccctccca gcgggtgagc cacaaagccc ttgcccactc cggcgaagac
     6361 gcgcacatat ggatcgagta cttcctctgg ccagaaatga taggcagcac ttccttccgc
     6421 ctgaagagca gaggcaagaa gcattcgccc gtgtatctca ggagtaacgc gaaacaccag
     6481 aggaacattc aaccccagat tgggcgccac cacgaacgtc tccggaattt gcaggcgtgc
     6541 ctttatgtcc gtatagctga gagctcctcc gaaaaaccag tggtccatgc gtctttcgta
     6601 taaaaacccg tactgtctgg aggataactc cagaggtgga actttgtaga ttggcccggt
     6661 ataacggagc gcaggcgaaa tgcggggagc atacaataca ttagaattca caagcaattc
     6721 taaaaggcga agccctgccc aggaactggc tacaatcgat ccggatgcgc tgacggcggg
     6781 acctgcgtga atcgagacat agggagtggg gctcggactc tggggttcgg gtagagcggg
     6841 atctgcctgg gcccacagag aatgcgcgca aagcgcaaca gaaaccagaa ggcaagcgcg
     6901 catcatcgga gcatcaggaa acagtgacgt tcaaggaata gaaaaaaaag ggggcttacg
     6961 ccccccgcct ttcatttagc aaagaaaagc taataatcaa tcactggtct actcatcgac
     7021 ccaggggaga cacgacttaa cgcacgggcc ccacttttcc tgggcaggaa gcttcacttc
     7081 tgccggccca aaccgtgcct tgtagttcat ggcatggcag gcagagataa aatatccagg
     7141 gtagtaatgc gaaagtccgc gagccttgca gagatcgatc tctgccagaa tggaaaaggt
     7201 gccgggacta tagcgggaat agtccggatc aaagaaaaaa tacacggagg aaagggcatc
     7261 ccccactaca tcgaaaatgc caaggcccac caggcgatct cctgcataga gcctcaattc
     7321 ctccgtagtc gcaagaaaac tgcttacaaa gaaatcggaa tatctgcaag gatccatgtc
     7381 gtcgcaggat ccgtgctggc atgccagata cttgcagtag agctgttcct tttctctgga
     7441 ataagaaggc ggcccggttt ccactctgaa aacagactga cccttcttcc agactctctt
     7501 ctggcttcgc gtgggtgcaa acgtggccac aggcaggcgg aggatcttgc actcctggca
     7561 gtttgcgcag tccggacgat atacgtggaa tccgtgcctt ctgtagccgc ggtcgagcaa
     7621 tagcctgtaa cccgcgccaa ggcccgtcgc ctcaagaaag agcaaattcg attctttctc
     7681 cggcaaatag gagcatgccc ccttcaggtg gcctgcgtat aattgtaatt cgagatattc
     7741 cagggactgg ctcatttggc tttgactgag agctctactt tagaaaatca gacgaaatga
     7801 gacgagtcta taacttttct ccaggtccgg ccgctcttcc gctgccagtt ctggaaacca
     7861 tccgcgatga gctgctcgat taccagaata cagggatgtc catcatggaa atgtcccacc
     7921 ggggtaaggt ttttgagggc ttgctggatg atatcaacca gagactgcgc aaactggcat
     7981 ccatcccgga tcgctttgat atactgtaca tgacaggcgg ggcatctgcc cagttcgctt
     8041 ttgttcccat gaacctctca ggcaaaggcc gcaaagcagg ctacgtcaat accggagtct
     8101 ggtccgaaaa ggcaatcgaa caggccaatt tgctcggaat ccaggtcttt gacgcaggga
     8161 gctccaaaga taagaaccac agctacatcc ccgaggtaaa gaccacctca ggcctggatt
     8221 acttgcatgt gacctctaac aacacaatct acggaaccca gttccagaag ctccccagtc
     8281 ctggagatac aaagctcgtc atagacatga gctctgactt tctatctcgt cccattgact
     8341 ggtctaacat cggaatggta tacgctggcg cgcagaagaa tgcgggtcca tccggagtga
     8401 ccatcgttgt agtggatcgt gaatactatg gaagagagtc tgcagaaact ccaactgtat
     8461 tccgctacag cacgtacgcg aaaaacaaca gcctctacaa cactcctccc acctttcaaa
     8521 tgtacgtgtt tggtctggtg gtttcctgga ttgaaaaaca gggcggcctg gaagagatgc
     8581 acaggtacaa ccagcacaag gctgaactga tctacaatgt aatggacgaa tttcctaagt
     8641 tctacacagg acatgcggtg aagaatgcca gatctctcat gaacattacg tttagacttc
     8701 catcagaaga cctcgaagga aagttcttga aggcagcaga agaaagaaag ctcatgggat
     8761 tgaaaggtca ccgctcagta ggcggactgc gcgcttccac ctacaatgca gtaggcgaag
     8821 aagcctgcca ggttctctcc gtattgatgc gtgaatttgc aagatcgaac ggaggatagg
     8881 atgctgtggc tcagacttgg tgacaacgaa ctcgttcaac tggatcacgt cgtttccatc
     8941 aagaagcaag agccacaggc catcgaacta acttttctcg aaccaaacgg gaagcgcatc
     9001 ctcaccttcc cggatccagc agaacgcaac agagcgtttg acaggctcct tgagaacctg
     9061 gtgaagctga ggatggctct ggaataatca gccgcacttt ttcttttgaa gaattcgcgc
     9121 tgcgaaaacg ctgcggtttc tcagaaatag gcgatcgcga tgccaatggc gttcgatcct
     9181 gcgggtattg tgccatttga agtcaggttg cccgtggaag tatttacgga gtacatggac
     9241 acattgttgg cagatccgtt tcccgaataa gcgaactttc ctgatggatc taccgcaatc
     9301 gcttgagcac cagtgcccgc agcaaccgta ctatttgccg taagaactcc cgtgacggtg
     9361 ttgatcgtgt acatagaaag attattggaa gtaaagttcg cagcaaaagc aaactgcccg
     9421 cttgaatgag atgcaattcc tatggggcct gttcccgcag cgattgtacc attggccgag
     9481 agaacgcctg aacccgggtt gattgtgtac atggacacat tgttcgaagc gttgttcgta
     9541 acatacgcaa acttacccgt cgggtctgca acaacaccca tgggccctgt cccggcaccg
     9601 atcgtgccgt tcgcggacag aactccggtc accgcattga ttgtgtacat ggacacatta
     9661 ttcgaagtgt tgtttgccac atacgcaaac ttacctgttg gatctacagc aatgcccaga
     9721 caacctgttc ccgcagcgat cgtaccgttt ggcgaaagca tgccagttgc ctgatttacg
     9781 gtgaacatgg agacgttgtt tgacgcgttg ttgactgcat aaataaaatt gccaccggga
     9841 tccactgcta agccctgagt gctcgaaccc gcggctatgg ttccaataga tgtgagagat
     9901 ccagttgtct ggttgatgga atacatcgat acgctattgg agccgttatt ggcggcatac
     9961 acaaatcttc cgctcggatc tacgacaatg tctcgaggct gagtacccgc cgcgatagta
    10021 ccgtttgcgg aaagcacacc agtcgtctga ttgatcgtat acatcgatac ggtggcagat
    10081 gagaagttgg tcacatacac aaatcgcgga taccgcggca tcatcgcgct tccgatcatc
    10141 gcgccgagca gtagatatgc gccgttcaca tctctattgc ttatggggaa agagcccgta
    10201 cacgagaaag cgaaaattag agacgtttga gccaccgctg catatgccct tgcggcggaa
    10261 cctgagagcc ggactatgcg ctttgagatc gatcttcccg acaagcaacg catacttcat
    10321 tctcgcgtgt ttttcgggtc aggtcaacaa gaaaaggttg attgcgcagc acggaaacat
    10381 ctcaacgccc caatccggtt caagggcacg ttgaaattga gacca
//
LOCUS       DAOQJL010000065        10384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3337_length_10384_cov_4.384452, whole genome shotgun sequence.
ACCESSION   DAOQJL010000065 DAOQJL010000000
VERSION     DAOQJL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_10384_cov_4.384452"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLG78_04520"
     CDS             complement(<1..274)
                     /locus_tag="PLG78_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE22402.1"
                     /translation="MIHPEGFFKQAWNALILVCTLFFAIEIPMRMALGYHIGGWLFAI
                     DVFITVCFMVDVVINFFTAEYVNGVLVTDKKVIAITYLKGWFLIDFL"
     gene            complement(329..1495)
                     /locus_tag="PLG78_04525"
     CDS             complement(329..1495)
                     /locus_tag="PLG78_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNE22403.1"
                     /translation="MSSWKKAYNPDHLPGFIETGIILLIILVIGHTITEDIAVIYHWD
                     HRWMFYLTVAGFVFDLIFTAEFAARSIITLKHGHFKHYVTQERGWIDALTSFPLLLLV
                     SGPALVVYLTDAHAEGAFFSFLIILKTAKAIRVTRVLRLIRVIKIFGKIQNTESQMTN
                     RHVATISTIAVVSLVSVLVIAQFVPFLHIGDHEAYRQRRVSELSSLFQAASAFPEGVD
                     RSSGIQKMIRAEGKGDVIKIKRANGEVVFEADPGESHDLMFTAYPDSIVIPGTDAQVN
                     LSFHGADAEHARLNIIVLLCILSIIGSFMLLYTRIFAQQIADPIFVMDKGLREWSYNL
                     EVRTLPGRDKEEVFRLAQVYNNRWLPLKSQIHSYRKTQGGETAEKSVLSLDGLL"
     gene            complement(1498..2433)
                     /locus_tag="PLG78_04530"
     CDS             complement(1498..2433)
                     /locus_tag="PLG78_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22404.1"
                     /translation="MSEEKKIMSEFVGKLWANPALAGASVVRRENQILSFIRENQTQL
                     QQAFAKPGYFPDLGWDGSIRLLFSVLTDLIVDSVEPRLDKILGALLNPEVLPQLEIRP
                     QEVDRERIKDFILKGLRNKATRDPYIAVLETISYGLYDRYVPKALARHKVIHNEFYRK
                     DRLSLPAPIMIAYLQLCSLFRPLYWQKVATASNNQLNLAAVAKDPRMFDSTYKFLNAF
                     LVQEMGGVPDRILRVGLESCMNAEEHPDTGGSSRLIGILVARAAEFDPTQKQDRGAES
                     PDKSWFHIHKKTAKYYGYDARYLDELYQIASEEAW"
     gene            2555..3355
                     /locus_tag="PLG78_04535"
     CDS             2555..3355
                     /locus_tag="PLG78_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22405.1"
                     /translation="MRIRETLLILMLASSPALAQPAPLTGMVGAGMGYYERRTSDQSI
                     RAYTVPVFLPLEATLGRFYLTGSLEYARREMEGATEASSEGLNFVNMEGRFRAIDSRN
                     LRLTFMEIAGVSTARGSSSLPPESRLADGYRFDSAMSLQYRIERLSFELKGGQIANRT
                     RGDFRPGDIRSGGLSMGFGLGAYNNEAWPVNLVLGVTSQFYGADRFAGEDLPGTEYGT
                     VFFSPGLMLSGKSLALRAGLDFPIRHLGAEDAYRDKVRANIGLRYFLQ"
     gene            complement(3361..4812)
                     /locus_tag="PLG78_04540"
     CDS             complement(3361..4812)
                     /locus_tag="PLG78_04540"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22406.1"
                     /translation="MIRSLFSLFLLIPGVSLPDADVRRGIVLLTITPVVEDATRPWIK
                     DQGTPFQRLGLVVGKGKILALESEVRGAAIVEVRKHSSTEAVTATIERSDRETDLAIL
                     SVPADFAQDLNPIPAGPDPVVGETLESIRVDGGLFRLQRNEVQFSEIEVTTATGFTAF
                     PVGNVASLEPFPEGGILISKGKLAGFVHDRSNGRRIDFLLPGYIHQFLAGGAPGSAGF
                     DYESLVDPVLRRNAGVEGRSGPMVTRIYPGTSAYGVLQKGDIILAVDGVVLDERGFFE
                     DGKNGRQRIELLLYAKGGVTRKIGDTVPIRIIRAARRMDVSFKLIRNEGNAVRVPIRE
                     REQGFLVEGGVVFLNLSLFFLQSQFGATYATSAPELAYLYQSKRFRDQPGDDRIVIIA
                     RVLPDDMTLGYESLGGAQVLRAGNETVKSVNHLHDIMEAAAKKDATLTLELSNGRMMV
                     LDLKNRNAINSRIRKRYGVPVDFRRASNSGPGG"
     gene            complement(4809..6398)
                     /locus_tag="PLG78_04545"
     CDS             complement(4809..6398)
                     /locus_tag="PLG78_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE22407.1"
                     /translation="MMWNLLQLIRPAVIGRAFRRNSSLLSKKICIPEARSFASVVASI
                     LILFLSGSIQAEDVAVARRAVVRIDISFFEYSYDTPYRAPAVRRASGTGFIIAGKRIL
                     TNAHVVSQSNTIRLHRPDQRQDYPAHIVFIAHDCDLALLEADDDAFYKDTQPLEIGVS
                     PELNSPVVVVGFPIGGDRVSITRGVVSRKDMDTYAHSTVDSHATIQVDAAINPGNSGG
                     PALQNDKVIGVAFQSLTRGENLGYLIPPEVIRKFLTDVSDGRYDGYIDFGTMDMTTKN
                     PILRSALGLDADSVDTGVLVYDVLQGSSADGFIERGDVLLQVNGKKLSFEGEVDWNGG
                     LHPYIEMLDNLASGTIIEVELLRKGKKVTVKFPARKSKVVDALRINYDTAPPYWIEAG
                     HVFQPLDANLMQQNSRNWAREGRADLTYYYNNFLSAGIYRSVDGFVMLTQRLADPSNI
                     YNDRFVSQVVESVNGEKVKSFTHFSSLMAAAHKEPKITIRFRDSAVPLVLETASLSQT
                     NERIRKAYGLTSLNFVPGEKK"
     gene            complement(6301..6879)
                     /locus_tag="PLG78_04550"
     CDS             complement(6301..6879)
                     /locus_tag="PLG78_04550"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNE22408.1"
                     /translation="MKKLEMADVIPNTEYEKIRDSFRSKILRIKEPRRIAVGPYLMFL
                     FENRETMLYQVQEMMRAEGIRAEEAIQHEINTYNELVPGPCELRATLMIEFADEATRR
                     VKLKELVGLEKHVSLVIDRDYVISAVFDEKQMDPDKLSSVQYIHFPLGEKAAEAFLST
                     NDVELVTTHPACSYRQSISQEQLSSLKEDLYT"
     gene            complement(6894..8261)
                     /locus_tag="PLG78_04555"
     CDS             complement(6894..8261)
                     /locus_tag="PLG78_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNE22409.1"
                     /translation="MNARINYENTSDTVYDPGNSLYWDVDSTDRELARAFDLCNGCRM
                     CFKYCPSFPTLFQLMDEKQDVFALSAEDKDKIVGECYQCKICYVKCPYTDRDHHKFNL
                     DYPALLQRAVHVKARKEGVPFRDKFLANSDFAGKTAGLLSGLANKAMKSNFHRRIMEA
                     ILGIHRNKRMPEFHSTSFMRWFKKKPAVPVQAGQEKVVLFSTCFVNYNNPQLGRDTVE
                     VLEKNQVHVDCPEQNCCGMPGLNSGDLDWAISKMKKNVESLYPYAKEGYRILAINPTC
                     SLTLKKEYVSFLPPELKEKAQAVASATMDVHEYLFELKKQEKLNRDFNSSPGKVGYHV
                     PCHLRAQNIGYRSRDILRLIPGAEIAVVDECCGHNGTWAMKEEYFEMSLEAGKRAFDG
                     LKEKEASHIATDCPLAAIQIEQGMGLGERPDHPVQILARAYRKPEDGGFPDAISSEEN
                     GQTGS"
     gene            complement(8321..8740)
                     /locus_tag="PLG78_04560"
     CDS             complement(8321..8740)
                     /locus_tag="PLG78_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNE22410.1"
                     /translation="MSNLKGSKTHENLKAAFAGESQANRRYLYFAKQADVEGYPDLAG
                     LFRDTAEGETGHAHGHLEYMRKVGDPATGMPIGDTALNLKSAIAGETHEYTDMYPGMA
                     KTAREEGFTEIADWFETLAKAEKSHAGRFEQGLKTLV"
     gene            complement(8819..>10384)
                     /gene="thrS"
                     /locus_tag="PLG78_04565"
     CDS             complement(8819..>10384)
                     /gene="thrS"
                     /locus_tag="PLG78_04565"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNE22411.1"
                     /translation="IERLEKEGQGYKVEMARDLKAQGETELSFYENTVKQGGAEKSAV
                     FVDMCNGPHVEHTGQLGAFKLVSVSGAYWKGRTDQPQLQRIAGYLFATEEELRTHERN
                     VEEAKKRDHRRLGVDLELFTTSEKIGPGLILWLPRGNVIKEELEGWAKDTEEKHGYQR
                     VTTPVITKEDLFYTSEHLPHYKESMFPPMRLDEENYYIKPMNCPFHHTIFSMRPRSYR
                     ELPLRIAEYGLCHRYEDSGSLMGLMRVRGMSMNDAHIYCSEDDAVEEFLEVLKLHEYY
                     YRTLGIEKYHMVLALRNPKNDKYHGDSEMWARAEALTRKAMETSGIAYEVENDGAAFY
                     GPKVDFQIVSSIGREFTASTCQLDLFMPEKFDLKFVDKDGSMKRPACLHRSPLGTHER
                     FVGFLIEHFGGAFPLWLAPEQVRILPVSENVHAYCQELASACKKAGLRVTLDDSGETL
                     GKRIRNAEKMKIPRMVVIGDVEKQKNVLNSRDYFSGEKTEISLDAFIAEALDEIRTKK
                     LRQIAKPLLVDAG"
BASE COUNT         2563 a         2799 c         2396 g         2626 t
ORIGIN      
        1 cgagaaagtc gatcaaaaac catcccttca agtaggtgat ggcgatgact ttcttatctg
       61 taaccaggac accattgacg tattccgccg tgaagaaatt gattacgacg tcgaccatga
      121 agcagactgt gatgaaaaca tcaatggcaa agagccagcc accaatatga tagccgagcg
      181 ccatacgcat cgggatttcg atggcaaaaa acaatgtgca gaccagaatg agcgcattcc
      241 acgcctgctt aaaaaatccc tctggatgga tcatacccgc tattaaaatc gggcaatgaa
      301 ggctcgttct tgaaagggct ctgaggtttt agagcagacc atccagagag agtaccgatt
      361 tctcggctgt ttcgcctccc tgggtctttc tataagaatg aatctggctc ttaagcggca
      421 accagcggtt gttgtatacc tgagccagtc ggaagacttc ttctttatct cgtccaggca
      481 aagtccgaac ctccaggttg tagctccact cgcgaaggcc tttgtccatg acgaagatcg
      541 gatctgcaat ttgctgagcg aagatgcgag tgtacagcag catgaaagac ccaatgatgg
      601 aaaggatgca caaaagaacg atgatattca ggcgcgcatg ctctgcgtct gcgccgtgaa
      661 aagaaagatt tacctgagcg tccgtacctg gaatgacaat cgaatccgga taggcggtga
      721 acatcaaatc atggctttcg ccgggatccg cttcgaacac cacttcaccg tttgcgcgct
      781 tgatcttgat cacgtctccc ttgccttccg cgcgaatcat cttttgaatc ccactgcttc
      841 tatccacacc ttccgggaac gcactggctg cctgaaagag agaagacaat tcggatacac
      901 gacgctggcg ataggcctca tgatccccta tatgcaagaa cggcacgaac tgggcaatca
      961 ccagaacact gacgagagaa acaacagcaa tggtagaaat cgtcgcaaca tggcgattgg
     1021 tcatttgaga ttctgtattc tgaatcttac caaaaatctt gatcacccga atcaatcgca
     1081 gcacgcgtgt tacgcgaatc gctttcgcag ttttcaggat gatcaagaaa ctaaagaaag
     1141 caccttccgc atgcgcatcc gtcaggtaaa ccaccagggc cggacccgaa acaagcagaa
     1201 gaagcggaaa agaagtgagg gcatcgatcc accccctctc ttgcgttacg tagtgcttga
     1261 aatgaccatg cttcagagtg atgatagaac gtgctgcaaa ctcagcggtg aaaatcaaat
     1321 caaatacaaa tccagcaact gtcagataga acatccatcg atggtcccag tgataaatca
     1381 ctgcaatgtc ttccgtaatt gtgtgcccga tcaccaggat gatcagtaag ataatgccgg
     1441 tctcgatgaa accaggaagg tgatccggat tgtatgcctt cttccagctg ctcatactca
     1501 ccacgcctct tcgcttgcaa tctgatagag ttcgtccaga tagcgtgcgt cgtatccgta
     1561 atactttgct gttttcttat gaatatgaaa ccaggatttg tcgggacttt ccgcgccgcg
     1621 gtcctgtttc tgggtcggat caaattcagc tgcgcgtgcc actagaatcc cgatcagccg
     1681 cgaggaccca ccggtgtccg gatgctcttc tgcattcatg cagctttcga gccccacccg
     1741 cagaattcga tccggaactc cgcccatctc ttgcaccaga aacgcattga ggaatttata
     1801 agtggaatcg aacatcctgg gatctttcgc aacggccgcc aggttcaact gattgttaga
     1861 cgctgtggcc actttctgcc agtagagggg acgaaacaga gagcacagtt gcagataggc
     1921 aatcatgatg ggcgccggaa gactcaatcg atcctttcta tagaattcat tgtggatcac
     1981 tttgtgcctt gcgagtgctt tgggtacgta gcgatcatag aggccgtagg aaatcgtttc
     2041 cagcacggca atgtatgggt cgcgagttgc tttgtttcga agacctttga gtataaaatc
     2101 cttgatgcgt tcgcggtcca cttcctgggg tctgatttcc agctgcggca atacttccgg
     2161 gttcaaaaga gcgcccagga tcttgtccag gcgtggttct acggaatcta cgattaaatc
     2221 cgtgagtaca gaaaacagaa gccggataga gccgtcccag cccagatccg ggaaataacc
     2281 cggtttggcg aatgcctgtt gcagctgcgt ctggttttcc cgaatgaagc tgaggatctg
     2341 gttttcgcgg cggacgaccg aggcacctgc cagggcagga tttgcccaga gcttgcccac
     2401 gaattccgac attatctttt tttcttcact catggaacga cgatactata ctatcggttt
     2461 gtgatggcaa tttttcgcgc aataggcaaa agttacttga aaagcataga aatcgggcgc
     2521 aatcaaaaaa atctgaccgt ctaacctatt agtcgtgcga atacgtgaaa ccttactaat
     2581 cctgatgctc gcttcatcgc ctgctctcgc tcagccagct cccctcacgg ggatggtagg
     2641 agcgggtatg ggttactatg agcgccgcac atcagaccag tccattcgcg cctacaccgt
     2701 acccgttttc ctgccactcg aagccaccct gggaaggttc tacctcactg gctccctgga
     2761 gtacgccaga cgcgagatgg aaggcgccac tgaggcatct tcagaggggc tgaatttcgt
     2821 gaatatggaa ggccgctttc gcgccatcga ttccagaaac ctcagactga cttttatgga
     2881 gatcgcggga gtatctacag ccaggggttc ctccagtctc ccgcccgagt cgcgcctggc
     2941 agatggatac agatttgact ctgccatgag tctgcaatat cgcattgaaa ggctcagctt
     3001 cgaattgaag ggcggtcaaa ttgcaaatcg caccagaggg gactttcgtc caggagatat
     3061 cagatccggt ggcctgtcga tgggatttgg tctgggtgcc tacaacaatg aggcatggcc
     3121 cgtgaacctg gttcttggcg tcacttcgca gttttatggc gcggatcgtt tcgcaggcga
     3181 agatctgcct ggaactgaat acggaactgt ttttttctcc cccggcctca tgctctccgg
     3241 aaaatccctg gcactcagag caggacttga cttcccgatt cggcacctgg gagcagaaga
     3301 tgcataccgc gacaaagtgc gcgcaaacat tggcctgcgt tactttctac aataacggct
     3361 tcaacctcca ggaccagaat tagaagcacg tctaaagtct acgggcactc cgtatcgttt
     3421 tctgattcta ctgttgattg cgtttcgatt tttcaaatcc agaaccatca ttcttccatt
     3481 ggataattca agagtgagcg tagcgtcctt cttcgcggcc gcttccatga tgtcatgaag
     3541 gtgattgacg ctcttcactg tttcatttcc agcgcgaaga acttgcgctc ctccgagaga
     3601 ttcatagccc agggtcatat catccggcag aacgcgcgca atgattacaa tgcgatcatc
     3661 tcctggctga tcccggaagc gtttgctctg gtaaagatag gcgagctcag gcgcagaagt
     3721 ggcatacgtt gctccaaact ggctttgcaa aaaaaacaga gacaaattca agaacactac
     3781 gccgccttcc acaagaaagc cctgctctct ttcgcggatc ggcacccgca ccgcatttcc
     3841 ctcatttcga atcaatttaa acgatacgtc cattcgcctg gccgcgcgaa tgatccgaat
     3901 gggcacagta tctccaattt tgcgagtcac gccacctttc gcgtacaaca gaagttcgat
     3961 tctctgcctt ccgttctttc cgtcttcgaa aaagcccctc tcatccagca cgactccgtc
     4021 gacggcaaga atgatgtctc ctttctgtaa cacaccgtag gcgctcgttc ctgggtagat
     4081 gcgagtgacc attggtccac ttctcccttc cacacctgcg ttgcgtctga gtacaggatc
     4141 caccagagat tcgtagtcaa agcctgctga acctggcgca cccccggcca gaaactggtg
     4201 aatgtatccc ggcaatagaa agtcaatccg cctgccgttg gatcgatcat gaacaaaacc
     4261 tgccagcttg cctttcgaaa tcagaattcc gccttcagga aaaggctcaa gcgatgccac
     4321 gttgcctaca gggaaggcag tgaaccctgt agcggtggtc acttcgattt ctgaaaactg
     4381 aacttcattt ctctgaagac gaaacaaacc tccgtccaca cgaatcgact caagcgtttc
     4441 acccaccaca ggatctggcc cggcaggaat tggattcaaa tcctgcgcaa agtcagcggg
     4501 aacagagaga atggctaaat ccgtttcgcg atccgaacgt tcaatcgtcg cagtcaccgc
     4561 ttccgtggaa gaatgctttc gcacttctac aatcgccgct ccgcgcactt cgctttcaag
     4621 ggcaaggatc ttgccttttc caacgaccag tcccagcctc tgaaatggag tgccctggtc
     4681 tttgatccag ggtctggtgg catcttccac aacaggagtg atcgtaagca ggacaatgcc
     4741 ccggcgaacg tccgcgtctg gcaaagaaac acccggaatc agaagaaaca gagaaaacag
     4801 gctgcgaatc atttcttttc tccgggaaca aaattcaacg atgtaagtcc gtacgctttg
     4861 cgaatccttt cgtttgtttg cgaaagagaa gccgtctcga gaaccaatgg aactgcagag
     4921 tcacgaaaac gaatcgtaat cttaggctct ttgtgagccg cggccataag cgatgagaaa
     4981 tgcgtgaaac tttttacttt ctctccattc acagactcta cgacctgcga aacaaaacga
     5041 tcgttataaa tgttggaagg atccgccaga cgttgagtca gcatcacaaa gccatctaca
     5101 gatcgataaa tgcctgcact cagaaagttg ttgtagtaat atgtaagatc cgcccttcct
     5161 tcgcgcgccc agtttcgcga attttgctgc atcaggtttg catccagagg ctgaaacaca
     5221 tgacccgctt cgatccagta aggcggagcg gtatcatagt tgattcgcag tgcatctacc
     5281 accttcgatt tcctggcagg gaattttaca gttaccttct tcccttttct cagaagttcc
     5341 acttcaatga tggttccgga tgcaagatta tcaagcatct caatgtacgg gtgcagacct
     5401 ccgttccagt ccacctctcc ctcgaaggat agcttcttgc cattcacctg cagcaaaacg
     5461 tcaccgcgtt ctataaagcc atccgcggaa cttccctgca gcacatcata cacgagcaca
     5521 cctgtatcca cactgtctgc atcgaggcca agggcagatc gaagaattgg attcttcgta
     5581 gtcatgtcca ttgtgccaaa gtcaatgtat ccgtcatatc gaccatcaga cacatccgtc
     5641 aggaactttc gaatgacctc cggaggaatc aaatacccca ggttctcacc gcgagttaga
     5701 gactgaaatg caacaccgat tactttatca ttttgaagtg ccggaccgcc agagtttcct
     5761 ggattgatcg ccgcatccac ctggatcgtc gcatgcgaat ccacagtact gtgcgcatat
     5821 gtgtccatgt ctttgcgcga aactacacct ctcgtgatgg aaactctgtc ccctccgatc
     5881 ggaaaaccaa ctacgacgac tggagaattg agttccggcg aaaccccgat ctccaggggc
     5941 tgcgtatctt tatagaaagc atcatcatcc gcttcgagca atgcaagatc gcaatcatgt
     6001 gcaatgaata caatgtgtgc tggatagtcc tggcgctggt ctggtctgtg aagacggatg
     6061 gtattggact gactgaccac atgagcattc gtcagaatgc gcttgcctgc aatgataaat
     6121 ccagttccac tcgcacgtct gactgcaggc gctcgatagg gagtgtcata agaatactca
     6181 aaaaaactga tgtcaatgcg aaccaccgct cgcctggcca cagctacgtc ctctgcctgg
     6241 atggaaccgg acaggaacag aatcagaatc gatgcaacaa cgctcgcgaa gctacgagcc
     6301 tcaggtatac agatcttctt tgagagaaga gagctgttcc tgcgaaatgc tctgccgata
     6361 actgcaggcc ggatgagttg taacaagttc cacatcattg gtggacagga aagcttccgc
     6421 cgctttttct cccagcggaa aatgaatgta ctggacgctc gacaacttgt ccggatccat
     6481 ctgtttctca tcaaaaacag ctgaaatcac gtaatcccgg tcaatcacca ggctaacgtg
     6541 cttttccagg ccaacgagtt ccttaagctt caccctgcgt gtggcctcat ctgcaaattc
     6601 aatcatcaaa gtagcgcgca attcgcaggg tcccggtacc agttcgttgt atgtgttgat
     6661 ttcgtgctgg attgcttctt ctgcgcgaat tccctctgct cgcatcattt cctgcacctg
     6721 ataaagcatg gtttcgcgat tttcaaaaag aaacatcaag tacggtccta ctgcgatgcg
     6781 ccgcggctcc ttgattcgca gaatctttga acgaaagctg tcccgaatct tctcgtattc
     6841 tgtgttcgga attacgtctg ccatttcaag tttcttcatg gtgcgctcct tgtttacgaa
     6901 ccagtctgac cattctcttc agatgaaatc gcatccggga aacctccgtc ctccggttta
     6961 cgataagcac gcgcaagaat ctgaactgga tgatccggtc tctcacccag acccataccc
     7021 tgctcaatct gaatcgccgc cagggggcag tccgttgcaa tgtgtgaggc ctctttttct
     7081 ttgagcccat caaatgcacg cttccctgct tcaagactca tttcaaaata ttcttccttc
     7141 atagcccagg ttccgttgtg accacagcat tcatccacaa cggcaatttc agctcccgga
     7201 atcagccgaa gaatatcccg tgacctgtac ccaatgttct gcgcgcgtaa atggcatggc
     7261 acatgatagc ccaccttgcc cggtgaactg ttgaaatcgc gattgagctt ctcctgcttc
     7321 ttgagctcaa acaaatactc gtgcacatcc atggtcgcac tggccactgc ctgcgctttt
     7381 tcttttagct caggcggaag aaagcttacg tattcctttt tcaaggtaag cgagcaagta
     7441 gggttgattg caagaatgcg atacccttcc ttagcataag gatacaggct ttccacattc
     7501 ttcttcattt ttgagatagc ccagtctaga tccccgctgt ttaaaccggg cataccgcaa
     7561 caattttgct ccgggcaatc cacatgcact tgattcttct cgagcacttc tactgtgtca
     7621 cgaccaagct gcggattgtt gtaatttacg aaacatgttg aaaagagaac tactttctcc
     7681 tggccggcct gaacaggaac tgcgggcttc tttttgaacc agcgcatgaa gctggtggaa
     7741 tggaactccg gcattcgttt gttgcgatga atcccaagaa ttgcttccat aatgcgacga
     7801 tggaaattgc tcttcatcgc tttgtttgca agaccggaaa gtaaacccgc agtcttccct
     7861 gcaaaatcag agttggccag aaacttgtcg cgaaatggaa ctccttcctt ccttgccttt
     7921 acatgaaccg cacgctgtag gagagcagga tagtccagat tgaatttatg atgatcacga
     7981 tctgtgtaag gacatttgac atagcagatt ttgcactgat agcattcccc gacgatcttg
     8041 tccttgtcct ctgcactcaa tgcgaagacg tcctgcttct catccatcag ttgaaagaga
     8101 gttgggaaag aggggcagta tttgaaacac atccggcagc cattgcatag atcaaatgca
     8161 cgcgccagct cacgatccgt cgaatccaca tcccagtaga gagaattgcc tggatcatac
     8221 accgtatcag atgtattctc ataattaata cgcgcgttca tgaaagactc cggaaaaatt
     8281 ctgcgcctgt cctgaggaca ggcgctctta gtgtgatcag ttatacgaga gttttcagac
     8341 cctgttcaaa gcggcctgcg tgagactttt ctgctttcgc gagagtttca aaccaatctg
     8401 cgatttcagt gaatccttct tcgcgcgctg tcttagccat accaggatac atatccgtgt
     8461 attcatgcgt ttcgcccgcg attgctgatt tgagattcag agcagtgtcg ccaatcggca
     8521 tgccagtagc tggatcccca acctttctca tgtattccag gtgaccgtgt gcgtgtccgg
     8581 tttcaccttc tgctgtgtcg cggaaaagac ctgccagatc aggatatcct tctacgtccg
     8641 cttgctttgc aaagtacagg taacgcctgt tcgcctggga ttccccggca aatgcggcct
     8701 tcaggttctc atgtgttttt gagcctttga gattcgacat tcagtcctcc gatcactcat
     8761 ttagagtaat tcttgatttg gaataaatcc aaagccggtt tattgtccag ctaatttttt
     8821 atcctgcatc aaccaggagc ggctttgcga tctgtctaag cttttttgtg cgaatctcgt
     8881 cgagcgcttc cgcgataaag gcatcgagcg aaatctctgt cttttcgccc gagaaataat
     8941 cccggctgtt caacacattt ttctgcttct ctacatctcc gatcactacc atgcgaggga
     9001 tcttcatttt ctcagcgttt cgaatgcgtt tccccagagt ttctccggaa tcatcgagcg
     9061 tgactcggag ccctgccttt ttgcaggcgg aggcgagctc ctgacaataa gcatgcacgt
     9121 tttcgctcac cggcagaatc cgaacctgct cgggcgctag ccacaatgga aaggccccgc
     9181 cgaaatgctc gatgagaaat ccaacaaagc gctcatgcgt gcccagtgga ctcctgtgaa
     9241 gacaggccgg ccgtttcatg ctgccgtctt tgtccacgaa cttgagatca aacttttcag
     9301 gcatgaaaag atccagctgg caagtgctcg ctgtgaactc tcttcctata gaagaaacta
     9361 tctgaaaatc gacctttggc ccatagaagg cagcgccatc gttctccacc tcataggcga
     9421 ttccggacgt ttccatagcc ttacgagtca gagcttcggc tctcgcccac atttctgaat
     9481 cgccgtggta cttgtcgttc tttggattgc gaagcgccag caccatatga tacttttcta
     9541 tccccagagt gcgatagtag tactcatgga gctttagaac ttcgagaaat tcctccaccg
     9601 cgtcatcttc tgaacaatag atgtgcgcat cgttcataga cattccccga acgcgcatca
     9661 ggcccatgag ggaaccggag tcttcatacc tgtgacacag accatattca gcaatgcgaa
     9721 gaggtagttc acgatagctt cgtggacgca tggaaaagat cgtatgatga aacggacagt
     9781 tcatcggctt gatgtagtaa ttctcttcat ccaatctcat cggaggaaac atagactcct
     9841 tgtaatgagg aaggtgttca ctggtataga agagatcttc cttggtaatc accggagtcg
     9901 tgactctctg atagccgtgt ttctcctctg tgtccttggc ccatccctcg agctcttctt
     9961 tgatcacgtt accgcgtgga agccacaaaa tcaaaccagg cccaatcttc tctgatgtag
    10021 taaagagctc tagatccacg cccagtcttc tatgatcgcg cttcttggct tcctccacat
    10081 tcctttcatg agtacgcagt tcttcctctg tggcaaacaa gtagcctgca atccgttgca
    10141 gttgaggctg atctgttctc cctttccagt aagccccaga cacactcacc agtttgaacg
    10201 cgcctagctg tccggtatgc tccacatgag gtccgttgca catgtctacg aagaccgccg
    10261 atttctcggc accgccctgc ttcacagtat tctcgtagaa agacagctca gtttcgccct
    10321 gggccttgag gtcgcgcgcc atttccacct tataaccctg accttctttc tccaatctct
    10381 cgat
//
LOCUS       DAOQJL010000066        10383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3338_length_10383_cov_4.275368, whole genome shotgun sequence.
ACCESSION   DAOQJL010000066 DAOQJL010000000
VERSION     DAOQJL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_10383_cov_4.275368"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLG78_04570"
     CDS             <1..527
                     /locus_tag="PLG78_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22412.1"
                     /translation="GDWNGYFFFRNLPGVIHAGGAEASGTYKLPRLSITLSHSVVRVL
                     TVSDSMYFSPYNGGMYLTNKKFNPHFRAFPENITRAHVSFTPRNWTFGVNYLYYANWY
                     SPVGNRIEGNHILNGNVGYTIGPRMEISLIVKNMLAEGALYPMNSNAGDAAVSDGSPA
                     LEGRTYLVQFRYSF"
     gene            complement(537..905)
                     /locus_tag="PLG78_04575"
     CDS             complement(537..905)
                     /locus_tag="PLG78_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22413.1"
                     /translation="MKREMVGFRSAILASVFAAHCTGAYGGIDGPSVIARNEAVSRIN
                     DAKLARMAVCGYNSAGAISLYNDYVRLPRKVLDGAYYATRDVDRCVESIWLTPCNQWP
                     LDCGLSPKEIAEPPLLEGSI"
     gene            complement(984..4571)
                     /locus_tag="PLG78_04580"
     CDS             complement(984..4571)
                     /locus_tag="PLG78_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22414.1"
                     /translation="MIPFPTISESPTAWEYFWHQLPWAVTGFLTFVVGVSLAAVGVYA
                     ALRRTDKGLLINFVLFSLGYGCLGLVLGFRAVILDRDLLLLLNRIPYFAVVLLSPGGY
                     LFTYYLTDQRYKSLLYAAGAAVVTVVLSIGGLIIGYDFTGEWFHYPFGNFPIAHFPLR
                     LWGLVSALGYLITAVPMTFRYYRDHRADLWEKRYLFIGLHLTSILIISNLPSLAGIPL
                     FPMSSFAFIPLLIMAYGIFHSDFLSVNDLLFQKRGLFKLLAGLVTSGFIIAALATAAE
                     INPTQHIALYLKPTFLIPLFSGVCAFSLAIFLAGSNPDQKLSMLAAASLMLTGAFMVV
                     MTTIKLDLSPVVTRRIEQIMYTFFVFTPSVHMRFAFHSMKQPSPRFVRLFDIAGVISA
                     IVLWTPYFFSGFYEYSFGRISVGGIGLNLYGFVGVVCAATFLFTWWKTSRKEKTPGGL
                     VIFALVVGDLLILLNLPATMGIPLYTLGDLQFIPAFILAFAVLNQGAISVEGEATAIG
                     NRISVLILLFVPVSMLFYFISLAEQGVPALAAISHIALVASPVALAFYMISFVFLRPT
                     ALKMDETIRQLAHEKEKTDAALNETERARREIEALSQFTHLINSYTDLDKIFRAISEY
                     VYTQFSISALWLFLPDEKREFLQSYKAYSYNKIPEESYRKLRNLRLPLNENGGIAWAV
                     WERKKSLYLPKIKRYAFGVDREIVALSGATSFLHVPLVVNDRAVGLMAFSNVEENMKL
                     KPREIRTIEAFCAQIAGVVNTAHLLKTTEKQKLDTENLNRFIKSLNEELDMDVIMKKI
                     HEYVKETFQIQYYALYTLDATRSHFESVQIELPPGVSDQDALSIKKITFPVKKGRGAN
                     GYALRSKKPFYTPVVENLLSLATPDEQFVVEKCRVRSMILLPLVLNGEAIGVLNLSNS
                     DEEMNLSQEDITRLSILAEHLAGIVFGSNLFQQVRVAKNQADEERGKSERLLLNILPG
                     DVAAELKEKGATEPVLYESVSVMFTDFKGFTQIAETMTPRELIQDLDACFVQFDKITE
                     RFNLEKLKTIGDSYMCAGGIPKRNTTHAVDCVLAALEIQAFMNLMRDIKENQGLPYWE
                     LRLGIHTGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGVPGRINISGTTYEEVKDLF
                     ECEYRGLVNAKNKGEVAMYYVLGIKPEFAQDGDPRAPNAKFWQQYATVT"
     gene            complement(4897..6546)
                     /gene="groL"
                     /locus_tag="PLG78_04585"
     CDS             complement(4897..6546)
                     /gene="groL"
                     /locus_tag="PLG78_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770238.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNE22415.1"
                     /translation="MAKQLDFHEDARRKLQAGVNKLANAVRVTLGPRGRNVVIDKKFG
                     SPTVTKDGVTVAKEIELEDHFENMGAQMAKEVATKTNDVAGDGTTTATVLAQSIVNEG
                     LKNVTAGANPMHLKRGIDKAVEAVVAEVKKRARKIGTNKDEIAAVASISANNDYEIGK
                     LIADAMAKVGNDGVITVEEAKGIDTTMEVVEGMQFDRGYQSPYFVTDPEAMIATMDKP
                     YILIHEKKIANMRELLPVLEKVAQSGRGLVIISEEVEGEALATLVVNNLRKTIRVVAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLDNADMSQLGQANKVVVDKENTTIIE
                     GAGKDADIKGRIAQIRKQIDDTTSDYDREKLQERLAKLSGGVAVVNVGAATEVEMKEK
                     KARVEDALSATRSAVEEGIVPGGGLTLLRAREVLKELKLEGDEQTGANIVYRALEEPL
                     RQIANNSGQEGSVIVQKAMTEKDNIGYNAATLVWEDLVKAGILDPAKVVRSSLQNAAS
                     IGGMILTTEVAITDLPEKDKGGMPGGMGGMGGMGGMGGMDM"
     gene            complement(6557..6841)
                     /locus_tag="PLG78_04590"
     CDS             complement(6557..6841)
                     /locus_tag="PLG78_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE22416.1"
                     /translation="MTIKPLGDRVLVRPLSQSEEKVGSLFIPDTAKEKPQEGMVEAVG
                     PGRTEDGKTIAVEVKVGDRVLYGKYSGTEIKKDGKEMLIIRESDILAVLS"
     gene            complement(7014..9878)
                     /locus_tag="PLG78_04595"
     CDS             complement(7014..9878)
                     /locus_tag="PLG78_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE22417.1"
                     /translation="MAFLFCATLAGSEARPLSVSIGRFEAFHSATRPDLEIRLRASLQ
                     KSLERNGIKGILLSASGDALAEARSQKTDFLVSGQYSSAKGLSLSAQIYDAQEGVVID
                     AFSLSDERASLSGLLSESEIAALGPPNEPETVENFSARIASRILTNPNKTRRPENISQ
                     FLDGTAVGRELKFPRSVVSVRDDAFRMLEEQRIAIGTRGTQTLREVPATIRVITDEDI
                     RRHRYQYLEDLLRDQPGFDMIFFQGVYGPIFMQQGIDGPENFRTLVFVDGVPDNNISS
                     GSAYIKHKYRLHNVKRVEILYGPASSLYGANAFSGVINIITKDGSDIQKVIEAGVGSL
                     YYESTFHRPAPNGFLTIGGVAMPGEDAISVIASAHWINSEGPILNQKSNVDPTKSTYY
                     WTDGYSNSRLSDNYMASLKMQYRSVTLGMWQERDYTGQGTFASAAAYLDDGRKTFWNT
                     LTTTYYGKFDQPIGMLQEHLQASYRETSVTDGADVDRSTIGSFNGKVTATRYRRPDSE
                     ISLDNPLQIKWSNNSYTTVGAAYVRQQPWTYNTRAVTYDSTAALYLIGMPASPDLDPR
                     NKFYATSKAGYAEHYMRLAGAFGTSVGYRYDHFTIEGKDGPEFCGTDEISLPGGAPNP
                     HFISPEAAQAAGCVLNERRQYYSHRAGDRNYDSSNPRIGLIYSSSSLLTAKLSVGEAF
                     RVPTVRELYSLSSSRISNSKLDPEKIRTAEASVIYYPTETIRTESVIFASYVRDLISL
                     AATDVHTPGKSQSTYLSAFQNAGSAQIYGTQLSAEGDILPWLRALWNYTYQQSWIFDA
                     KDSNLLAHSGAIDESLRPSDTTICRNTLVSTGYSQFNPLCPRAHGRMPRIASHKMTAG
                     VTILLSQHWQIDTRLNYVGSRRTIATDPVTSVPSYVLANISLGTKDFPLKGMDITLKV
                     FNAFNEAILDPGTRDGRGDYFPSVHPQPERYLGWDLTYRL"
     gene            complement(10024..>10383)
                     /locus_tag="PLG78_04600"
     CDS             complement(10024..>10383)
                     /locus_tag="PLG78_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22418.1"
                     /translation="TNPSDDSKRYKRKPYKVPGVTGVVAISGGPGHIIAQLKDGRLIG
                     WGEGYHGSLGNGSSDGIFPVPVSPTGLGPVVAHYKALRTSFAVLADGRVFVWGPLRMN
                     TGAGVLAGSATPVAFRP"
BASE COUNT         2404 a         2582 c         2686 g         2711 t
ORIGIN      
        1 cgggagactg gaatggttat ttctttttca gaaatctacc tggtgtaatc catgccgggg
       61 gtgcggaggc aagcgggacc tacaagcttc cccggttatc tatcactctt tcgcattcag
      121 tcgtgcgtgt tctgactgtt tcggatagta tgtattttag tccgtacaat ggcgggatgt
      181 atcttacaaa caagaaattc aaccctcact ttcgcgcgtt tccggagaat ataacgcgtg
      241 cacatgtttc ctttacaccg cgaaactgga cgtttggagt gaactacttg tactatgcaa
      301 actggtattc gcccgttgga aatcgcatag agggaaatca cattctgaac ggaaacgttg
      361 gttatacgat cggccccaga atggaaatca gtttgatcgt caagaatatg ctcgcagaag
      421 gagctctcta tccgatgaac agcaacgctg gggatgcagc tgtttcagat ggttccccgg
      481 ctctggaagg acgcacgtat ctggttcaat ttagatattc tttttgagga gcagcatcat
      541 atgctgccct cgagcaaagg tggctccgcg atttccttgg gggaaagtcc gcaatcgagt
      601 ggccactggt tgcacggagt aagccagatg ctctccacac accgatccac atcccttgtg
      661 gcatagtagg cgccatccag aacctttcga ggaagacgaa cgtagtcatt gtagagagaa
      721 atcgcacccg cagaattgta gccgcagact gccattctgg ccagcttcgc atcgttgatt
      781 cgtgagactg cctcattgcg agcaatgaca gaaggtccgt ctattccgcc gtaggcgccc
      841 gtacaatgag cggcgaacac agacgccaga atggcagatc gaaatccaac catttccctt
      901 ttcatccatg ctcccacttc tgctgtatga actcagtgtt tgctttgcgc aattcaaaaa
      961 actgttctgc ggaaccgttt gtgctaggtg actgtagcgt attgttgcca gaattttgcg
     1021 ttaggcgctc ttggatctcc gtcctgcgcg aattctggtt tgatcccgag aacgtagtac
     1081 attgccactt cgcctttgtt tttcgcattg accaggccgc ggtattcgca ttcaaaaagg
     1141 tctttgacct cttcgtaggt tgtgccgctg atattgatcc tgccaggtac tccagatgac
     1201 tccattctcg atgcagtatt gaccgtgtcg ccccacacat cataagcgaa ttttttctct
     1261 ccgattacac cggccacaag cggacccgtg tggattccga gacggagttc ccagtaggga
     1321 aggccctgat tttccttgat atcgcgcatc agattcatga aagcctggat ttccagagcg
     1381 gccagaacac agtccacggc gtgcgttgtg tttctttttg gaatcccacc cgcacacatg
     1441 tagctgtctc cgattgtttt tagtttctcg aggttgaatc gttctgtgat cttgtcgaat
     1501 tgcacgaagc atgcatccag atcctgaatc aattcgcgcg gagtcatggt ttctgcgatt
     1561 tgcgtaaatc ctttgaagtc cgtgaacatc acactgacgc tttcgtatag caccggttct
     1621 gtggcacctt tctctttcag ttccgccgca acatcgccag gcagaatgtt tagtagcagt
     1681 cgttcagatt tgccgcgctc ttcgtccgct tgatttttgg ccactcgcac ctgctgaaac
     1741 aggttcgatc cgaatacaat tcctgccagg tgttcggcca gaatggacag tcgagtgatg
     1801 tcttcctgac tcaagttcat ttcttcgtcg gagttggaga gattcagtac cccgatggct
     1861 tccccgttca ggactagagg caacaggatc atggaacgaa ccctgcattt ctctactaca
     1921 aattgttcat caggcgtggc cagggagagc aggttctcaa cgactggcgt gtaaaatggt
     1981 ttcttacttc gcagagcgta gccattcgcg cctcgccctt tcttaactgg aaatgtgatc
     2041 ttcttgatgc tgagcgcatc ctggtcactc actccaggtg gcagttcaat ctgaacagac
     2101 tcgaagtggc tgcgcgttgc atccagagtg tagagggcat agtactggat ttgaaaggtc
     2161 tctttgacgt attcatgaat cttcttcatg atgacgtcca tgtctagctc ctcattgagg
     2221 ctcttgatga agcgattcag attttctgta tccagttttt gtttttccgt cgtcttgagg
     2281 aggtgtgcgg tattgaccac tcctgcaatc tgcgcgcaaa aagcttcgat ggttcgaatc
     2341 tcccgtggtt tgagtttcat attctcttcc acgttcgaaa aggccatgag gcccacggcc
     2401 cgatcattca ctacaagcgg cacatgcagg aaggaagtag caccggaaag cgcaacgatc
     2461 tccctgtcga cgccaaaggc atacctcttg atctttggaa gatagagtga tttctttctc
     2521 tcccacactg cccacgcaat ccccccattt tcattcaacg ggaggcgaag gttgcgaagc
     2581 ttgcggtagc tttcctcggg aattttgttg taactgtatg ccttgtagct ttgtaagaat
     2641 tctctttttt catccggcaa gaaaagccag agcgcggaga tggagaattg agtgtacacg
     2701 tattctgaga tggctctaaa tatcttatcc agatccgtgt atgaattgat caaatgcgta
     2761 aactgactga gtgcctctat ttctcgcctg gctctttccg tttcattgag agccgcgtct
     2821 gttttttctt tttcatgcgc gagctgtcgg atcgtttcat ccatcttgag tgcagttggt
     2881 cgcaaaaaca cgaaagaaat catgtagaat gcaagggcaa ctggagaagc aaccagtgca
     2941 atgtgcgaaa tagcggccag agcgggcact ccttgctctg ccagagaaat gaaataaaat
     3001 aacatactca ccggcacgaa caggagaatc aaaacagaga ttcgattccc gatcgccgtt
     3061 gcttccccct ctactgaaat ggctccctgg ttcaacactg cgaaggccag gatgaaggct
     3121 ggtatgaatt gaagatcgcc taacgtatag agtggaatac ccatagtggc cgggaggttg
     3181 agaagaatga gtaggtctcc gacgacaaga gcgaatatga ccaggccgcc cggtgttttt
     3241 tctttgcgac tggttttcca ccaggtgaac agaaaggtgg cggcgcaaac aactccaaca
     3301 aatccataca gattcaggcc aattccacct actgagattc ttccaaaaga atattcatag
     3361 aaaccggaaa agaagtaggg cgtccacagc acgatggcag aaatcacgcc ggcaatatca
     3421 aacagtctta cgaatcgtgg gctgggttgc ttcatggaat gaaatgcgaa gcgcatgtgc
     3481 acactgggag taaaaacaaa gaatgtgtac atgatctgtt cgattctgcg cgtaacaaca
     3541 ggagacaggt cgagtttgat ggtagtcatc acaaccatga atgctcccgt gagcatcaag
     3601 gaagcagcgg cgagcatgct cagcttctgg tctggattgc ttcccgccag aaaaatagcc
     3661 agactgaatg cgcaaactcc agagaacaat ggaatgagga acgttggctt taagtagagc
     3721 gcaatgtgtt gtgttggatt gatctctgct gcggtcgcga gggccgcgat gataaatcca
     3781 ctcgtgacca gtcccgccag aagtttgaag agtcctcttt tttgaaaaag aagatcgttc
     3841 acgctcagaa aatcagagtg aaagattccg taagccatga tcaacagtgg aataaacgcg
     3901 aagctgctca tagggaagag tggaattcca gcgaggctcg gaagattgga aatgatcaag
     3961 atagacgtta gatgcaatcc gatgaacagg tagcgttttt cccagaggtc cgcacgatga
     4021 tctctgtaat agcgaaaggt cataggcaca gcagtgatca ggtaaccaag tgcggaaacc
     4081 aggccccata aacgcagagg gaagtgcgcg atgggaaaat ttccaaacgg atagtggaac
     4141 cactctcccg tgaaatcata cccaatgatc aatccgccaa tgctcagcac gactgtgact
     4201 acagccgcac ctgcagcata gagaagagat ttatatctct ggtctgtcag gtagtaggtg
     4261 aagaggtagc ctcccggcga gagaagcaca acggcaaagt acggaattct attgagcagt
     4321 agtaagagat cgcgatccag aatcaccgcg cgaaaaccca gaaccagtcc caggcatccg
     4381 taacccagag agaagagaac aaaattgatc agcaggcctt tgtctgttct gcgaagagct
     4441 gcatacacgc cgaccgcggc aagagacacg ccaacgacga acgttagaaa tccagtgacg
     4501 gcccacggta gctggtgcca gaaatattcc cacgcagtgg gcgattcgga aatggtaggg
     4561 aagggaatca tgttctttcg ggagccaggt atggatggat tgcgtgccag gtcaaactcg
     4621 aagagttgcg agaggccgga cgtcgcataa cgtgttcgcg ccgccgctag tccatcactt
     4681 cgctcacacg gaacgtggcg aatagcagcc tgcagttttg cctgcaacat gtttgcggca
     4741 caaaaacctt gatcgtaaaa gaggaaagcc ccgactcgct tcgtaaaccc tccacgaggg
     4801 agggttgtcc cgcaaccggg ccatggaggg cctgcttgcg ggaccgaagc gagtcggggc
     4861 tttctttgat ttgtgtttgc gcgtttggga cgcgaattac atgtccattc cgcccattcc
     4921 gcccatacca cccatgccgc ccattccacc aggcattccg cctttgtctt tttctgggag
     4981 gtcagtgatg gcaacttccg ttgtgaggat cataccgcca atggaagcag cgttctggag
     5041 agagctgcga accactttcg ctggatcgag aatgcccgct ttcacgaggt cttcccatac
     5101 cagagttgcg gcgttgtatc caatgttgtc cttctcagtc attgctttct ggacaatcac
     5161 cgatccttcc tggccggagt tgtttgcgat ctggcgaaga ggttcttcta gagcgcgata
     5221 tacgatgttc gctccagttt gctcatcgcc ttcgagcttg agttctttga gcacttcccg
     5281 cgcgcgaaga agagtgagtc cgccgcctgg cacaatgcct tcttctaccg cagagcgagt
     5341 agcagaaagt gcatcttcca cacgagcttt cttctctttc atttcgactt cagttgcagc
     5401 accaacgttt acaacagcaa cgccgcctga aagttttgcc aggcgttcct ggagtttttc
     5461 gcggtcgtag tcagatgtag tgtcgtcgat ttgctttctg atttgagcga tgcggccttt
     5521 gatgtccgcg tctttgcctg cgccttcgat gatggttgtg ttttctttgt ctacaaccac
     5581 tttgtttgcc tggccaagct ggctcatgtc tgcgttgtct agtttcagac cgaggtcttc
     5641 agaaatcacc tgaccgccag tcaggatcgc gatgtcttcg agcattgctt ttctgcgatc
     5701 gccaaagcct ggagctttta cagctaccac gcggatggtc ttgcgcagat tgtttacaac
     5761 gagagttgcg agagcttcgc cttccacttc ttcagaaatg atgacgagac cgcggccgga
     5821 ctgtgcaact ttctcgagca caggaagaag ttcgcgcatg tttgcgattt tcttctcgtg
     5881 aatgagaatg tatggcttgt ccatagtagc gatcattgct tccggatccg ttacgaagta
     5941 aggagactgg tagccgcgat cgaactgcat cccttcaact acttccatag ttgtgtcaat
     6001 gcctttggct tcttctacag tgatgacgcc atcgtttcca acttttgcca ttgcatctgc
     6061 gatgagtttg ccaatttcgt agtcgttgtt tgcagagata gaagcaacag ctgcaatttc
     6121 gtctttgttt gtgccgatct tgcgtgcgcg ctttttcact tcagcgacta ctgcttcaac
     6181 agctttgtcg attccgcgtt tcaggtgcat tgggttagcg cctgcagtta cgttcttgag
     6241 tccttcgtta acgatggatt gcgcgagaac agttgctgtt gtggttccgt cgccggcaac
     6301 gtcgtttgtt ttcgtggcca cttctttggc catttgcgcg cccatgttct caaaatgatc
     6361 ttcgagttcg atttcttttg cgacagttac accgtctttg gtcacagttg gtgaaccaaa
     6421 tttcttatcg atgaccacat tgcgtccgcg tggcccaagc gttacacgaa ctgcgtttgc
     6481 gagtttgttt acgcccgcct gcagtttgcg gcgcgcgtct tcgtgaaagt cgagttgttt
     6541 cgccatggtt tcctcctcag ctcagtactg cgagaatgtc gctctcgcgg atgatcagca
     6601 tttctttgcc gtcttttttg atttctgtgc cggagtattt tccgtacagt acacgatcgc
     6661 ctactttgac ttctaccgcg atggtcttgc cgtcttctgt acggcctgga cctacggctt
     6721 ccaccattcc ttcttgcggc ttttctttcg ccgtatctgg aataaatagg gatccaactt
     6781 tttcctcaga ctgggacaat ggacgaacca gtacccggtc tccgagaggc ttgattgtca
     6841 tgcattcctc ctgaatgatt tccaactcgc caggggcctg attagcagac tacccatgcg
     6901 agtgccaatt caagggccaa gaaactgaaa aatggtcctc agtcaagcga ttttggtaaa
     6961 aatctggcag tcgacggcat agagtgccag attcagaggt agaaataggg acctcacaaa
     7021 cgatacgtca aatcccagcc caaatatcgc tctggctgcg gatgaaccga cgggaaataa
     7081 tccccgcggc cgtctctcgt tccaggatcg aggatcgctt cattgaacgc attgaacact
     7141 ttgagagtga tgtccatccc tttgagaggg aagtcctttg ttcccaggct gatgtttgcc
     7201 agaacatagg agggaacaga agtcacagga tctgttgcaa tggttctgcg agatccaaca
     7261 tagttcaagc gagtgtcgat ctgccagtgt tgcgaaagta gaatcgtcac tcctgcagtc
     7321 attttgtgcg aagcgatgcg gggcattctt ccgtgcgcgc gcgggcagag tggattgaac
     7381 tggctgtacc cggtagagac cagggtgttt ctgcaaatcg tagtgtcaga aggcctgagc
     7441 gattcgtcga tcgcgccaga gtgagccagt agattggaat ccttcgcatc aaaaatccac
     7501 gattgttgat aggtataatt ccacaacgcg cgaagccagg gaagaatgtc tccttctgca
     7561 gaaagctgtg tgccgtaaat ctgcgcgcta cctgcgtttt gaaatgcgga gaggtaagta
     7621 gattgtgatt ttcccggtgt gtgaacgtcc gtggctgcca ggctgatgag atcgcgcacg
     7681 tagcttgcga agatcacgct ttctgtgcga attgtttctg ttggatagta gatgacgctt
     7741 gcttccgcgg ttctgatttt ttccggatcg agtttgctat tggaaatcct gctcgaagaa
     7801 agagaataca gttctcgcac agtggggacg cgaaatgctt ctcccactga cagctttgcc
     7861 gtgagcagag aagagctaga gtaaatcaga ccaatgcgag gattggaaga atcgtagttt
     7921 ctatctcccg cgcgatgcga atagtactgt cgtctttcat tgagaacaca gcctgcagcc
     7981 tgggctgcct ccggagaaat aaagtgagga ttcggcgctc ctcctggaag agagatctca
     8041 tctgttccgc agaattcggg cccgtctttt ccctcaatcg tgaaatggtc atagcgatag
     8101 ccaactgagg ttccgaatgc gcctgccaga cgcatgtagt gttccgcgta tcctgctttg
     8161 ctggttgcat agaatttgtt tctagggtcg aggtcaggcg atgcaggcat tccaatcaaa
     8221 taaagagcgg ctgtggaatc atacgtaact gcgcgagtat tgtaagtcca tggctgctga
     8281 cgaacgtagg ctgcgcccac ggtcgtatag gaattattag accatttgat ttgcaaagga
     8341 ttgtctagag agatctcaga gtccggcctt ctgtatctgg ttgccgtgac ttttccattg
     8401 aagctgccaa tggtactgcg atccacatct gctccgtctg taaccgatgt ttcgcgatag
     8461 gaagcctgca ggtgttcttg caacataccg atgggttgat cgaatttgcc gtagtaagtg
     8521 gtggtcagag tattccagaa tgtctttcgc ccatcgtcca gataggcagc ggcgctcgcg
     8581 aatgtgccct gtcccgtgta atctcgttct tgccacattc caagagtcac agagcgatac
     8641 tgcatcttga ggcttgccat gtagttgtcg ctcaggcgag aattgctata gccgtctgtc
     8701 cagtagtagg tgctctttgt cgggtctaca ttcgattttt gatttagaat ggggccttcg
     8761 ctgttgatcc agtgggcaga ggcaatcacg gaaattgcgt cctctcctgg catggcaacg
     8821 ccgcctatcg ttaaaaatcc gttgggcgct ggcctgtgaa aggttgattc atagtaaagg
     8881 cttcctactc ctgcttctat taccttctgg atgtccgaac cgtcctttgt gatgatgttg
     8941 atcacgcctg aaaatgcatt cgcaccatac aggctcgagg ccggaccata gagtatttct
     9001 acgcgtttta cattgtgcag tctatattta tgtttgatgt aggcgcttcc ggacgagatg
     9061 ttgttgtccg gtactccatc tacaaatacg agagtgcgaa agttttcggg cccgtcgatt
     9121 ccctgttgca tgaagatggg gccatacaca ccctgaaaga atatcatatc aaagcccggc
     9181 tgatcgcgca gcaagtcctc gagatactgg tatctatgtc tgcgaatgtc ttcgtccgtg
     9241 atgactcgaa tggtggccgg gacttcgcgc agagtctgag tgccgcgagt tccgatagcg
     9301 attctttgtt cttccagcat gcgaaacgca tcatctctca cactgactac agaacgtgga
     9361 aatttcagtt cgcgtcccac tgccgttcca tccaggaact gggaaatgtt ttctggcctt
     9421 ctggttttgt tcgggttggt caggatgcgc gaagcgattc gtgcggaaaa gttctctacc
     9481 gtttcgggtt cattgggcgg gccaagtgcc gcgatttcac tttctgatag aagaccagaa
     9541 agtgaagcac gctcatccga gagactgaag gcatcaatga cgacaccttc ctgcgcatcg
     9601 tagatctgcg cggaaagcga gagtcctttc gcggaagagt actggccaga gaccaggaaa
     9661 tctgttttct gcgagcgtgc ttctgccagt gcgtcgccag aagccgacag aagaattcct
     9721 ttgattccgt ttctctccag acttttttgc agcgaagcgc gaaggcgaat ttcaaggtca
     9781 ggtctggtcg cggagtggaa agcttcaaaa cggccaatgg acacgctgag aggcctggct
     9841 tcgctaccgg ccagcgtagc gcagaaaagg aaggccataa aaccgcgacg cgccacgtcc
     9901 gaaatttggc atggctcccg gactttgcaa ccgttaacct ggcctggcca ggtattatag
     9961 atccttaact attcgtaatg atcaggtgtt tggtcccggc agcgaccggc tctcttgcat
    10021 gctttacggt cgaaacgcga cgggcgtcgc ggaaccagcc aggacaccgg cgccagtgtt
    10081 catgcgaaga ggcccccaga caaaaaccct cccgtctgcg agaactgcaa acgaagtgcg
    10141 aagagccttg taatgggcca ccacaggtcc gagtccggtg ggagaaacgg gcaccgggaa
    10201 aatgccgtct gaactaccgt tgccaagcga gccgtgatag ccttctcccc atccaatcag
    10261 gcgtccatct ttgagctgtg caatgatgtg gcccggtccc ccggatatgg cgacgacccc
    10321 tgtgactccg ggcactttgt aaggtttcct tttgtatctc ttagaatcat cggatgggtt
    10381 ggt
//
LOCUS       DAOQJL010000067        10383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3339_length_10383_cov_3.691615, whole genome shotgun sequence.
ACCESSION   DAOQJL010000067 DAOQJL010000000
VERSION     DAOQJL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_10383_cov_3.691615"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLG78_04605"
     CDS             complement(<1..493)
                     /locus_tag="PLG78_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE22419.1"
                     /translation="MHNEKLATTRTTPCSTFKIWNTLIGAENHYLLSPDQPFYHWDGT
                     VREIPEWNKDLTLSEAFKVSCVPAFQDLARRTGRAKMETWIRLMPYGNGDLSSGIDDF
                     WLPRPGKASIQISPLEQALLMRRLVRGELPFSESSRNLLYKIMLVKKTPGGKLYGKTG
                     TGSL"
     gene            complement(652..945)
                     /locus_tag="PLG78_04610"
     CDS             complement(652..945)
                     /locus_tag="PLG78_04610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE22420.1"
                     /translation="MILEIAMLQVRPGLTQEFESNFAVASKILSSMPGYLGHSLRRCI
                     EEKNKYALLITWETLEAHTEGFRKSSGYLEWKALLYHFYDPFPIVEHFEDLSP"
     gene            complement(1183..1743)
                     /locus_tag="PLG78_04615"
     CDS             complement(1183..1743)
                     /locus_tag="PLG78_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22421.1"
                     /translation="MRFIKLLSLLLLTQCLTFNPIPGKEAAIDSAQIKLLSIFGPMDT
                     FSYGQFTYSPRDVTQAMWVFNVQYSGTGKVDPVAVSLEEYTMVDKGKESLNMPVRVHE
                     CCYSNSFGFSTNPFQNLANIVNRSRAGGSFMPDAAIPLQGGEPERRMLVFVFPRNKTP
                     TKLSFHPLRDVKDETAKIDLGLTNTP"
     gene            complement(1897..2160)
                     /locus_tag="PLG78_04620"
     CDS             complement(1897..2160)
                     /locus_tag="PLG78_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22422.1"
                     /translation="MLKKTANYLLLYGLLTFVFTAIYSRFTSSAYALGTATGIAFVSL
                     VLPFFIFGIRAIFNRKLSPRVITVSRFLWLVCWAGAVLRQVLR"
     gene            complement(2246..2716)
                     /locus_tag="PLG78_04625"
     CDS             complement(2246..2716)
                     /locus_tag="PLG78_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22423.1"
                     /translation="MNWREHPILKKLSPPDYPDDVPAIIHEGFRAATRTPEKVWIRIS
                     DMRDSILTGTLLNAPHQLPSLSQGKVYSFQLSGAGIPILVSESYLREIVDWRFAIACK
                     KCGNDVLFDNPSTIIRMAFPNMPEGSLMEAFSTFCPLCHDPIVLRHRNAPPDEP"
     gene            complement(2860..3141)
                     /locus_tag="PLG78_04630"
     CDS             complement(2860..3141)
                     /locus_tag="PLG78_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22424.1"
                     /translation="MNSTIRPFIVLIIAFAVTFCNGRQQFDPTGSREAEWKRRCQLNA
                     FIALSAMCAAYASRQTATAAYDPFTACFYESSFAFGSSCTEAAFRKNNE"
     gene            3482..3757
                     /locus_tag="PLG78_04635"
     CDS             3482..3757
                     /locus_tag="PLG78_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22425.1"
                     /translation="MDFEFYVDPDIGITHLEKHNVTPGEIQEFFAESRFLEHERYDAS
                     FEAIGRLTSGRYLHVAYRKKSRDLYFIITAYDLTDPDYKSMLDGLEE"
     gene            complement(3946..4599)
                     /locus_tag="PLG78_04640"
     CDS             complement(3946..4599)
                     /locus_tag="PLG78_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22426.1"
                     /translation="MKPENPYSAPAQSAPLPDSGNYSLTLRVFCFAAVLRFIFSLAST
                     ALATRHPEIKTSVIPMLVTVLMGLLDTAAHFQLFRMTRHKLLLLAIAFNLASYAHSAA
                     TGMGDLSVTGFVFYCLSNLGTAGAYWQLPVVPLRLRKFRWLTIALVVLVALNSVFWQS
                     SLNRELARSTWFLIFPFFGVTFFYLTMMFRTLWTFSTAHVASHSIAPHQREGLPRTS"
     gene            complement(4750..5448)
                     /locus_tag="PLG78_04645"
     CDS             complement(4750..5448)
                     /locus_tag="PLG78_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22427.1"
                     /translation="MRQRQGTSFISTVDAARFIPGIWDPFAYRTHAETTLGEIATIRR
                     PRRSRISSVLRPLEYRDIPKGAQLTYDLGFDDVTELQSAFASPGALLFGTMRAYLGNV
                     VVTPEFKWLGFAEPLVYPIKSEFVEITPHDSCTYFWWSLLRSPEFCRRLPLGGGGTRP
                     RLDPDLLAQTPLPQLDPVKIQEIDTRLRSLAKQAWEDYTQLMEILRAASDNGHIQTPE
                     APAPHQKLGHMHRL"
     gene            complement(5448..6362)
                     /locus_tag="PLG78_04650"
     CDS             complement(5448..6362)
                     /locus_tag="PLG78_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE22428.1"
                     /translation="MKSKPLAPLLTRAQLAPLSRHPRLEAALKEAILRDPSGANLPRW
                     YQHFIGRKYRGATGKFFTPVPVAAAMAALFPIPAQATLLDPACGAGTFLSAVKSQNTS
                     TPAKYVYNDIEPALIELARLGSALNGMQEGETDFYCENIFDPSTRLHATYKDKVDIIL
                     ANPPFSVYLTDVTFFSPIFSAGYHNSDAIFLDACLHFLKPGGKMALLLPHSLVANANF
                     RKFRIIVEKNWGLLGVIALPEGVFKLTAEATTRTDIVLLEKRTGPVGKRIFGYVQTVG
                     VRTTGRSSETVSNDLDSLVRSNPLGIGL"
     gene            complement(6706..8181)
                     /locus_tag="PLG78_04655"
     CDS             complement(6706..8181)
                     /locus_tag="PLG78_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE22429.1"
                     /translation="MHSLGKATTLFDLRFEKSLPDLQSFDAICLFGHPENSGQYRNWA
                     HTVIPTILAGAPESAAIIAGGMGFLDVSKAMTDYPQIDVVITGSPEIAISEIVQDHPL
                     DSVTNLALRTDTGFHETERKVLPLPENLFPNRMLRNPAYNYHMIGIKTDVVRTAVGCN
                     YRCKFCFEYGKASDGSFLRWQGRSPQSILSEIQTLDATIVGLIDDDLTTSMPMLDQLS
                     DLLIEKGIKKSYISVGRIDHVLNAAATLDKMERAGFLALSFGLESVQDRTLRMLGKGL
                     TLQKIELGLEALNKTNILIGGTFLLGSPGESEAEMMEILTFARKWNLDSVGTNRIRIE
                     ENRDQKAAAWKTAPAITGEELASIKRKIKYGQRTPFRILLSFCKLYRHKGMPFDPAYI
                     LIQMMETYTKGTFIHRVRLISRGFAILQKWLRLSYVRKVNRLIAFVLYPFLKFATIVF
                     ERIDNHLGIATNLLPLWFNWYKKTVYNRQTIAAQVHSASNN"
     gene            8176..8721
                     /locus_tag="PLG78_04660"
     CDS             8176..8721
                     /locus_tag="PLG78_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22430.1"
                     /translation="MQRGLHIFGGQRWKYIIWILERKRVHNCDIVEFAVFLGQAIFHK
                     FSSVALYGFTNKAMLPCGEVMGSIRSKRGEQGNPRAPGRQCLPLECRRKLSFAEELVF
                     PLFGRKPISFPFEERIGLLMTQNGPNACEKVDYLERNGGGEEQYIADRGEGKVENGEK
                     PFGKARAIFFFNNTGIIRGSR"
     gene            complement(8934..9545)
                     /locus_tag="PLG78_04665"
     CDS             complement(8934..9545)
                     /locus_tag="PLG78_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE22431.1"
                     /translation="MKREDGITAFYAPDRNTWRTWLTKNHSREKNVWLIIYKKTSKTK
                     SVSYAEAVEEALCFGWVDSKPNRRDSQSYFQFFARRNPKSNWSTKNRNTVEKLIRAKL
                     MTPSGLKAVKLARTAGTWDALSEVDAGQPPPDLVDALRRNRLAKKHFDAFPRSSKKII
                     LEWILNAKRAETRAHRISETVRLAAQNLRANHFRQPKRAGDSV"
     gene            complement(9881..>10383)
                     /locus_tag="PLG78_04670"
     CDS             complement(9881..>10383)
                     /locus_tag="PLG78_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22432.1"
                     /translation="LSGRFLPLKIIAASLVLNMLGFTFFSKPPQQFQYIARNAGFIAN
                     FPARPVEIPLAEGAISLVANGVSGPIGLTRIPLNDGQTSALRIDRLIRSMQSNDNRRM
                     ESRKNLTRRNLNTVEVIWQSQQARQITKFITLKGEILVLECSLPDDGTLASAEVATFL
                     EPEWFP"
BASE COUNT         2596 a         2423 c         2784 g         2580 t
ORIGIN      
        1 ccaaagagcc ggttcctgtt ttgccgtaga gcttccctcc gggggttttt tttaccagca
       61 tgatcttata tagtaaattt cttgaacttt ccgagaaagg caactcgcct ctcaccagtc
      121 ttcgcatcag gagagcctgt tcgaggggag agatctggat ggatgccttg ccagggcggg
      181 gcaaccagaa gtcgtctatg ccagaagaca aatccccgtt gccgtatggc atgagacgaa
      241 tccacgtttc cattttcgcg cgtcccgttc gccgggctaa atcctgaaat gcgggaacac
      301 aggacacctt gaatgcctcg gacagtgtga ggtccttgtt ccattctgga atctcacgca
      361 ctgtcccgtc ccagtgatag aatggttgat caggagacaa aagatagtga ttctcggcac
      421 ctatcagagt attccagatc ttgaatgtag agcagggggt ggttcgcgtc gtggccagtt
      481 tctcattatg catgaatctt tgctctgagt ccagatctag caggacaaaa gttgcgttcc
      541 ggttttgaag atgattctcc atagaacgtg ctatggattt tttttcagag caatcgcaga
      601 tgacgcacaa cgtcaaaaaa tatagcatcc acgttttttt caagggttcg ctcaaggact
      661 aagatcttcg aagtgctcta ctatgggaaa gggatcgtag aaatgataga gcaacgcctt
      721 ccactcaagg tatccggagg atttgcgaaa gccttctgtg tgtgcttcca gagtttccca
      781 cgtaatcaac aaagcgtatt tatttttttc ttcgatacat cttcgaagcg aatgacccag
      841 gtatccgggc atcgaggaca ggatctttga ggcgactgcg aaattggatt cgaattcttg
      901 tgtgaggcca ggtcgtacct gaagcattgc aatttcaaga atcatatggt tcgaccactg
      961 ctagcggtaa ggcaggcgca aatgtagagt tcatgacgcc ttgcaatctc ctgatacccg
     1021 gcgaaccgtg cgcgaaggac cagggagaat catgcatatc gcattctttc aggctcaata
     1081 ggagtctgtg gcgattccag tattcatgcg cgagtcaaag tccaattccg caaactgcca
     1141 cgattagtcc tggcccggct aagccgggca ggtttcgcgc tgttacggag tgttcgtcaa
     1201 accgaggtcg attttggccg tttcatcttt cacgtcgcgc agtgggtgaa agcttaattt
     1261 cgttggtgtc ttgttgcgcg gaaaaacgaa gacaagcatg cggcgctctg gctcgccgcc
     1321 ctgtagaggg atagcagcat ctggcatgaa gctcccgccc gccctggaac ggttgacgat
     1381 gtttgcaagg ttctgaaagg gattggttga aaagccaaaa gaatttgaat agcagcattc
     1441 gtgtacgcga accggcatat tcagggattc tttgccctta tccaccatcg tgtattcttc
     1501 caatgatacc gccactggat ccacttttcc cgttcctgaa tattgaacat tgaaaaccca
     1561 cattgcctga gtgacatcgc gcggactgta agtgaattgg ccgtagctaa atgtgtccat
     1621 aggtccgaaa atcgatagca acttgatctg ggcagagtca atggctgctt cttttcctgg
     1681 tatcgggttg aaggtgaggc attgagtcaa aagcagaagg ctgagaagtt tgatgaaacg
     1741 catcagcaat acagaatttg aagcgaaacc gcgtcaacca caatggatcc aaaccagaag
     1801 gtgtgccgag tgcttttgtt tagtggaaca gaacgtttga atgggacatg attcagtgcg
     1861 ctccaagaac gctcagcagt gctgagaatg ggccggtcat cggagtacct ggcgaagtac
     1921 ggcacctgcc caacagacaa gccagagaaa tcggctgacc gtgataacgc gcggcgatag
     1981 cttgcgatta aatatggccc gaattccaaa tatgaaaaac ggaagaacca gagagacaaa
     2041 agcaatgccg gtggcagtgc ccagggcgta ggccgaactg gtgaaccgag aataaatggc
     2101 agtaaagaca aacgtgagca gtccgtacaa caacaaatag tttgcagttt ttttcaacat
     2161 ggtttttccg tgcgacgcta gtgtgagacc actcggtccg cggttttcag attcgactgc
     2221 gtgtcgaggg gaatgttccg gtcttctaag gttcgtcagg tggcgcattc ctgtgacgaa
     2281 gaacgattgg atcatgacac agggggcaga aagttgaaaa ggcctccatg agactccctt
     2341 caggcatgtt tggaaatgcc attcgaataa ttgttgatgg gttatcaaac agaacgtcgt
     2401 tgccgcactt cttgcatgcg atggcgaaac gccaatcaac aatctcacgc aagtagcttt
     2461 cggaaacgag aattggaatg cccgcacccg agagctgaaa cgaataaacc ttgccctggc
     2521 taagtgacgg caattggtgc ggcgcattca gcagcgttcc ggtgaggatg gagtctctca
     2581 tatctgaaat gcggatccag accttttctg gagtacgagt ggcggcgcga aaaccctcat
     2641 ggatgatggc cggtacgtca tcaggatagt cgggcggact taatttcttg aggatgggat
     2701 gctctctcca attcataaaa gactccggtg gaatttctca gtgtgcttgc atagatttcg
     2761 aaattgagtc aaacactttg cgacacaatg ccaagtattg gctgcgccgg acctatcaga
     2821 aactaccaac gcctgacatg agttacgcga agtgccatgc tattcattgt tctttctgaa
     2881 tgcagcttcc gtgcagctgg aaccaaaggc gaatgacgac tcgtaaaaac aggcggtaaa
     2941 cggatcataa gctgcggttg cggtttggcg actcgcataa gctgcacaca tggcggaaag
     3001 agcgataaag gcgttcagct gacaccgccg tttccattca gcttctctgg atcccgttgg
     3061 gtcaaattgc tggcggccat tgcagaaggt cactgcaaaa gcaatgataa gcacgatgaa
     3121 cggtcgtatg gtggagttca ttcaggttga cccgcaaggt tttggatgca tgccggagtt
     3181 tcatcgagga tggtcgtggc agaattggtt ctatttatct gaatggttcg aaacaatcgt
     3241 agctcctttg tcttcatcca tgatcgagca ttggttcacg ttgagaagga aataacatgc
     3301 ctcgcttcct ccgtttggat cgccggatct gcatttcgca taacaggagt tatgcgccat
     3361 attcgcggcg ggggaatcgg aactgcttta tcgcgcccct tcacaaatcc aaaatcttgg
     3421 tcattcacgg gcttctggga acgcttgacg actgcggtaa aaaggccttc ctagtcccac
     3481 tttggatttc gaattctacg tcgatccgga catcgggatc acgcatcttg aaaagcacaa
     3541 cgtcacaccc ggagaaattc aggaattctt tgcggagagc agatttctag aacatgagcg
     3601 ctatgacgcg agttttgaag ctatcggccg cctcacatcg gggcgttatt tgcatgttgc
     3661 ataccgcaag aagtcccgag atctttactt cattatcacc gcttacgact tgacagatcc
     3721 cgattataag tctatgttag atgggttaga agaatgaaag tcatagaaac aaagacgttc
     3781 cgcgtttcct acgttgtgcc cgaaacggag gctgaaaaga cttcgataca gcgcgtcatt
     3841 gacgaaattc aaacggacaa agttccacgt tccgaatgga aaatcgtgga taatcgtcca
     3901 aagccccgcc gcgaacacgg cgcataactc gcgttaccac gttagttatg aagtgcgggg
     3961 cagcccttca cgttgatgcg gcgcaatgga gtgactggcc acatgtgcgg tggagaaggt
     4021 ccaaagcgtt ctgaacatca tggttaagta gaagaaagtt acaccgaaaa acgggaagat
     4081 aaggaaccac gtagaccgag ctaactctct gttcaatgag ctttgccaga acacagagtt
     4141 gagtgcgacg aggacgacga gtgctatggt caaccatcta aactttcgca accggagcgg
     4201 aacaacggga agttgccagt aggcgcccgc tgtgcccagg ttcgataaac agtagaatac
     4261 gaagccagtg accgaaaggt cgcccatgcc agttgcggcg ctatgtgcat aactggcgag
     4321 gttgaaggca atcgcaagga gcaagagttt gtggcgggtc atgcgaaaga gttggaaatg
     4381 ggccgccgtg tcgagaagac ccatcaggac ggtcactaac atcgggatca cactggtttt
     4441 gatctctgga tgccgggtgg caagagcggt gctggccaaa ctgaaaatga atcgaagcac
     4501 ggcagcgaaa caaaagacgc gtaacgtcag agaatagttg cccgagtcag gaaggggtgc
     4561 gctctgtgcg ggggcagagt atgggttttc aggtttcatt tcttctggcc acagcgtaat
     4621 cttctatcgt acgttcccag agagttgaag tgaagatgtg ctgttcgagt aaataatgtt
     4681 ttccatgggc ctattcgggg gcgggatggc gcataacgtg ttaacgcgag ctaaacgcac
     4741 gcgcgccggc tatagccggt gcatgtgccc gagtttctga tgcggcgcgg gcgcttcagg
     4801 tgtttggata tgcccattat cggaggcggc tctgagaatc tccattagtt gtgtatagtc
     4861 ctcccaggct tgctttgcca gggaacgaag tctcgtatct atctcttgaa ttttcacagg
     4921 gtctaactga ggtaaaggcg tttgcgcaag gagatcagga tccagacgcg ggcgagttcc
     4981 tccgccgccc aatggaaggc gacgacaaaa ctccgggctt cgcagcaaag accaccagaa
     5041 atacgtgcaa ctgtcatgcg gtgtgatttc aacaaattcg gattttatag gatatacgag
     5101 tggttctgcg aaacccaacc acttgaattc aggtgttact acaacattcc caagataggc
     5161 acgcattgtt ccgaacagca acgctccagg tgaggcaaag gccgactgta attctgtgac
     5221 atcatcgaat ccgagatcgt aagtcaattg cgcacctttt gggatatcgc ggtattcgag
     5281 cgggcggagt accgatgaga ttcgacttcg cctgggacga cgaatggttg cgatttcacc
     5341 gagtgtcgtt tccgcatgcg tgcggtaagc gaatggatcc catatccccg ggatgaacct
     5401 tgcggcatct accgttgaaa taaaactggt gccctgtctt tgacgcatta gagtccgatg
     5461 cccaatgggt tgcttctaac cagggaatca agatcgttcg aaaccgtttc gctacttctt
     5521 cccgtggtcc gaacacccac tgtttgaaca tatccgaaga tacgctttcc aaccggcccg
     5581 gtgcgcttct cgagcagcac aatgtctgtg cgcgtagttg cctcggcagt cagcttgaaa
     5641 actccctctg gtaaggcgat cactccgagg agaccccagt ttttctccac gatgattctg
     5701 aatttgcgga aatttgcgtt cgctacaagg gagtgaggaa gtagcagggc cattttgccc
     5761 ccgggtttga ggaaatgcag acaggcatcc aaaaagatcg catcgctatt atgataaccc
     5821 gctgaaaaga tggggctgaa aaatgtgacg tccgtaagat atactgaaaa aggagggttc
     5881 gcgagaatga tatcaacctt gtccttataa gttgcatgga gccgggtgga agggtcaaat
     5941 atgttttcgc aatagaaatc tgtttcccct tcttgcatcc cattgagcgc ggacccgagc
     6001 cgtgcaagct ctatgagagc aggctcaatg tcattgtaga cgtatttggc cggagtgctg
     6061 gtattttgcg atttgacggc actcaaaaat gtacctgctc cgcatgccgg atccagtaaa
     6121 gtagcctgcg ccggtatcgg aaacagagcc gccatagccg cagcaaccgg gacgggtgta
     6181 aaaaattttc cggttgcgcc gcgatacttg cgaccaatga aatgctggta ccatcgggga
     6241 agattggctc ccgaagggtc acggaggatt gcctccttga gcgcagcctc cagccgggga
     6301 tgcctcgaga gcggcgcgag ttgagccctg gtcagaagtg gtgctagagg cttgctcttc
     6361 aaaacaagcc tccaatagaa ctcgcaaatc agaaagaagc ctggctcgcc gtcatatgcc
     6421 aacaaacgaa ggtcttctac cagttgacga aattcacgac taattgcatg gtccacgttg
     6481 cttattttgc agattatgtc ccgaattgtc aactcgatca gagtgagctt gcaaccgggt
     6541 tttcaaccgg gattctgatt ccttgcgaat gcattccacc attggtaacg cgagtaatgt
     6601 gaaggcagcg atctgaatca gttggtgttt ctctccgcaa gccggacgtc tggagcttcg
     6661 tggcgttatg cgaagtgacg cctcttcttt tttttgctga acgaattaat tgttcgaggc
     6721 tgagtgaact tgcgcggcga tagtctgacg attgtaaacg gttttcttat accagttgaa
     6781 ccagagcgga agcaggttgg ttgcgatacc gaggtgattg tcaatccgtt cgaaaacgat
     6841 cgttgcaaat ttcaaaaatg gatagaggac gaaggcaatt agccgattga ccttgcgtac
     6901 gtatgataac ctcaaccatt tttgcaagat agcgaaacca cggcttatca ggcggaccct
     6961 gtgaatgaag gtaccttttg tgtatgtctc catcatttgg atcaggatat acgcgggatc
     7021 gaacggcatg cccttgtgcc gatacagctt acagaaactg agcagaattc taaacggtgt
     7081 gcgctgtcca tatttgattt tgcgtttgat tgaggccagt tcttccccgg tgatggcagg
     7141 tgcggttttc catgcggccg ctttttgatc gcgattttcc tcaatccgaa tccgattcgt
     7201 gccaacactg tcaaggttcc atttacgcgc gaacgttaga atctccatca tctcagcttc
     7261 ggattcgccg ggcgatccaa gcagaaacgt tccaccgatg agtatgtttg tcttgttcaa
     7321 tgcctcgagc ccgagttcaa ttttctgaag cgtaagtcct ttaccgagca tccggagagt
     7381 acgatcctga actgactcca atccaaaact cagggcgagg aaacctgctc gctccatttt
     7441 gtccaatgtg gccgcggcat tcagaacatg atcaattctc cccactgaaa tgtatgattt
     7501 cttgattcct ttctcaatca gtaaatcgga gagctgatcg agcatgggca tgctggtagt
     7561 caggtcgtcg tcaattagcc caacaatcgt tgcatcgaga gtttgaatct cactgaggat
     7621 actttgagga ctgcggccct gccacctcaa gaaggacccg tccgacgctt taccatattc
     7681 aaagcaaaat ttacatctgt aattgcaacc aacagcagtc cggactacat ctgtcttgat
     7741 tcctatcata tgatagttgt acgcgggatt gcgaagcatt ctattgggga aaagattttc
     7801 cgggagggga aggaccttcc gttcggtctc atgaaatcct gtgtctgttc gaagcgctag
     7861 attcgtaaca gaatccagcg gatgatcctg cacaatttct gaaatcgcga tttccggaga
     7921 accagtgatc accacatcaa tctgcggata atccgtcatt gctttcgaga cgtccaggaa
     7981 ccccatccca cccgcaatga ttgcggcaga ttcgggcgcg ccagccagta tggttggaat
     8041 cacggtatga gcccagtttc tgtattgccc gctattttca ggatgtccga aaagacagat
     8101 ggcgtcaaaa ctttgtaaat ccggaagcga tttctcgaag cgtagatcaa atagagtggt
     8161 tgctttaccg aggctatgca acgcggcctg cacatattcg gcggtcagcg gtggaagtac
     8221 ataatatgga ttttggaacg taaaagggta cacaactgcg atattgttga atttgcagtt
     8281 tttttgggcc aggcgatatt tcataaattc tcctcagtcg cgttgtacgg tttcactaat
     8341 aaagcaatgc tgccatgcgg ggaagtgatg ggttcaatcc gctcgaagcg cggtgagcaa
     8401 gggaatcctc gagctccggg acgccagtgc ttgcctttag aatgccggag aaaacttagc
     8461 tttgcagagg agctcgtttt ccccctgttc ggtcgcaagc cgatatcgtt ccctttcgaa
     8521 gaaaggatag ggcttctcat gacgcagaat ggccccaatg cgtgtgagaa agtcgattac
     8581 ctggagcgga acggtggcgg cgaggagcaa tacatcgccg accgcggaga agggaaagtc
     8641 gagaacggcg agaaaccgtt cggaaaagcg cgcgcaatct ttttcttcaa taatacggga
     8701 attataagag gttcccgata gaatgaaaat atctccggac cgccacgcat gaacggatcg
     8761 ggccgtagcg caatgcgaga gtaataatgc ggaagcaacg agtaatagaa caaagtttct
     8821 catttttcac agcagaaatg tcttagcacc ggggcgtagt tcacaggtcc ggtcttcggt
     8881 tccaaccgaa cgaatgcttg aattgcccca tttcgcataa cgtttaccgc tggttatacg
     8941 ctgtcgccgg cgcgcttcgg ttggcgaaag tgattggccc gtagattttg cgcggcaagc
     9001 cgcacagttt cagagattct atgtgctctc gtttctgcac gtttggcgtt gagaatccac
     9061 tcaagaataa tctttttgga ggagcgtgga aatgcatcga aatgtttttt cgcgagcctg
     9121 ttccgccgca gcgcatccac tagatccgga ggaggttgtc cggcgtccac ttccgagaga
     9181 gcgtcccagg tgccggcggt cctggcaagc tttacggctt tgagaccgga aggtgtcatg
     9241 agtttggcac ggataagctt ctcgacagtg tttctgttct tggtactcca gttgctctta
     9301 ggattgcgcc tcgcaaagaa ttgaaagtag ctctgcgaat ctcgccggtt tggcttgctg
     9361 tcgacccagc cgaaacagag ggcttcctct acggcttcag cgtaggagac gcttttagtt
     9421 ttgctcgtct ttttgtaaat gatgagccaa acgttttttt cccgggaatg gtttttggtg
     9481 agccatgtac gccaggtatt ccgatcaggg gcataaaaag ctgtgattcc atcttctcgt
     9541 ttcatatgca atggttgaat tgccagagat ggcgtatacg cagttcgccc ttagagattt
     9601 cggtatgccg cggaagatga gatgtagagg tcgagaattt tgcgctgagt ttttagcacc
     9661 aggatagttc ctttctgctc ttcgcgtcca cgttggagca agagtgagat ttgctggata
     9721 gccggtagat cagtgaggac gaatgttccg ccaacgcttc gcgaggggag aacgccatac
     9781 gcaacatttg cataggagcg ggatgtccaa aggaaagaga ttggcgaaag ttctggccgc
     9841 gttgttgtac tcgtcgccgg cgtcgatgcg aaggacagcc tcaagggaac cattcaggtt
     9901 ctagaaaagt tgcaacctct gcggaagcaa gagttccgtc gtcgggaaga ctgcattcga
     9961 gaaccagtat ttcacccttc agggtgatga atttcgtgat ttgacgagct tgctgagatt
    10021 gccaaatcac ttcgacagtg ttgaggtttc ttcgcgtcag gttttttcgt gattccatac
    10081 gtctattgtc attcgattgc atggaccgga tcaaacggtc tatccgcaga gcggaggttt
    10141 gaccatcgtt aagtggaatt cgagttaagc ctatcggtcc ggagaccccg ttggccacga
    10201 gcgagatggc tccttcagct agcggaattt caactggtct tgcagggaaa ttggcaatga
    10261 atcctgcgtt gcgagcgatg tattgaaatt gttgtggcgg tttggagaag aatgtgaacc
    10321 cgagcatatt gagcaccagg ctggctgcga ttatctttag agggagaaac cgtcccgaaa
    10381 gct
//
LOCUS       DAOQJL010000068        10358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3355_length_10358_cov_4.347083, whole genome shotgun sequence.
ACCESSION   DAOQJL010000068 DAOQJL010000000
VERSION     DAOQJL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_10358_cov_4.347083"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLG78_04675"
     CDS             <1..224
                     /locus_tag="PLG78_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22433.1"
                     /translation="TTGQPSAQSQAFSVSGGPYGLAMDPTNTYLFAAGGTTLYVFKRD
                     SAGTLTQIAQPTPGGAAFNKVAIYSTSNF"
     gene            complement(526..1698)
                     /locus_tag="PLG78_04680"
     CDS             complement(526..1698)
                     /locus_tag="PLG78_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE22434.1"
                     /translation="MRFLFSLLVVVSFATGCAKLRYALSGTCPEYDFGTPPGVSPPAQ
                     PPEVCMTPERVALGRSLFYDRRLSLNETQSCSTCHVAALGFADGKARPTGSTGQIHPR
                     NVPGLADVGYFSVLTWTLPELTRLENQTLIPFFSEKSDVTIEELNVSGKEYVVLDRLK
                     VDQNVRAMFEQAFPGEPIDISHIARALAVFQTTLISYQSPFDLGHMNDAAKRGYALFQ
                     SEKTGCFHCHGGRDFNQDDQAGKLWYQNVGLYNVSDAGDYPDHSLHGSAAARQTQGLS
                     LVTGKKEDRGKFRTPSLRNVSVTAPYMHDGSVATLEEAIEVFANGGRNIANGPFAGDG
                     TKNPNKDVRVRALPLSRQDKADLVEFLKALKDECFLSNPNFSDPGAPPPLQPEYCK"
     gene            complement(1767..2969)
                     /locus_tag="PLG78_04685"
     CDS             complement(1767..2969)
                     /locus_tag="PLG78_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22435.1"
                     /translation="MKSKRFLLLSAIVPFLVLGLSLRVTQEEHPAGLAVSAPDFSPIV
                     SSLQTLQTGTLHLTTAQPAWKFVMDGPQKLHATSANDWFAVRQSATWANSNSTLADNL
                     IMSLKNSGLLKNSGTVQSTATVNGVVYKVKLQTGGCGANCTGVTAAAASGLNFTNRFK
                     MWRASDNANVLELMFNDVTNPTSGILLTYNLGILSPTVSDNTNLIVESYISGASPSRH
                     QTYSWGAQFWLSGANAASSSDRGRVVLDEMTLGLKNGGTATGLCVRIAARTVSMTTGC
                     GTGNHYYALAYGQKTTSNFETTAQSGVALGSLATGGTVCGANILGIGIFNGGGFIQDG
                     LSASTVPTGYPDPSAGAGGYPGVQTVFGKINTAPNGAGTYDDLTLATINGLNTTVTFH
                     PTSDPPGF"
     gene            complement(2966..3166)
                     /locus_tag="PLG78_04690"
     CDS             complement(2966..3166)
                     /locus_tag="PLG78_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22436.1"
                     /translation="MVRKMAIILMVCVLGLGSCAFLNLDKKEEKDNTNLLLLLFGAAY
                     LNSQGCKNNSGLVICIPPGVPN"
     gene            complement(3259..3552)
                     /locus_tag="PLG78_04695"
     CDS             complement(3259..3552)
                     /locus_tag="PLG78_04695"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNE22437.1"
                     /translation="MREKLSESQIESMLQDTPGWAREGESIRRVFAFSDFQRAFSFMS
                     GCALVCEKADHHPDWSNSYNRVDVRFTTHDAGGLTLKDFELARAFNAFLMQKA"
     gene            3632..4309
                     /locus_tag="PLG78_04700"
     CDS             3632..4309
                     /locus_tag="PLG78_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22438.1"
                     /translation="MSRALLLLTLFAISCSSPYLRNRGRDALDIFTVEFQTRAYGASV
                     RAGPVKAGLYHKSADGAAAGLRGGDLGVHHDAEFAVLFFGADYFQKKPLGDLETPSEE
                     DSKTAEETDPSQTALRGKEFRARSPFGTTQPAQKKRSVFKKKGGFAPASYYTQLEVSL
                     GIFFGVKLGINPGELLDALLGWTTLDIFRDDEPFETEQEKALRDNPLYDRLTDEQRQK
                     LKEQMAK"
     gene            complement(4325..5446)
                     /locus_tag="PLG78_04705"
     CDS             complement(4325..5446)
                     /locus_tag="PLG78_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNE22439.1"
                     /translation="MNHRKCRGCSAVFAVTCLDNLLAAKITHLEILRQSLVLLEHGSA
                     RDKACAEQVHRRMNFAALGSVGPDVFYFYHVLSRKRNQLGLHWGNLSHHSRVFDLMIG
                     FLQRVKDMPEGIRREKCYAFAMGYISHCAVDIVTHPYIFYITGDYYSIDLKQAVRAQE
                     NHLRVEFQLDAYLVHHRWGLEPSRYNFAQYVDIHEETPDGPAMDYDLWSMWVDALAMV
                     YRDEFENTYFGSEARILPGDILNEAYVGFMKFNRILDTRSRPVRALLTAIDFATFHTI
                     KARYLLLPPPDQINPRLLNEERKEWKYPGDPQVTSKESFMDLVHRAAKFAADAMADAD
                     AFITGSKKLRDLAEKYDGYNLDTGLRSESIAMHEFDPID"
     gene            complement(5388..6017)
                     /gene="hisG"
                     /locus_tag="PLG78_04710"
     CDS             complement(5388..6017)
                     /gene="hisG"
                     /locus_tag="PLG78_04710"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNE22440.1"
                     /translation="MGVFTFALPAGRLALESIQFFRSSGLCDIQIPEGSRELCFTDPS
                     GEYRIILVRPTDVPTYIIQGGAHAGITGKDVLLESTFDVTTPLELGFGKCRLSVAGKP
                     DEKPLEKKHLRVATKYPVQAMNFLYQKGISCEIIKLHGSVEIAPALGLADCIVDLVST
                     GSTLRANGLVEQEVILNSEAVLVVGRFAYALESQKVQGMLSRFRSHLPG"
     gene            complement(6019..6726)
                     /locus_tag="PLG78_04715"
     CDS             complement(6019..6726)
                     /locus_tag="PLG78_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22441.1"
                     /translation="MRRKVITPEAVDPLRDFHGNQFERAVATAFYWMRQHSRQVVLGL
                     ILVVVVGTASLAFFIYRDYREQKSLTDFEELMKSPVMASGSGAEKVAIEKLEKYEREF
                     PNDHAQLRAGLKKVDLLIKTGEKEKAGDALVEIARIVETPELSALFSYRAGLLFEEKG
                     KFAKAESAYSNASQKLMDEGGAAALARFGQARALILLNKDREGREVMKKMLEIKDVES
                     IADLRIAAAAFLIQHGK"
     gene            complement(6728..8344)
                     /locus_tag="PLG78_04720"
     CDS             complement(6728..8344)
                     /locus_tag="PLG78_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE22442.1"
                     /translation="MLGALDGQGDVQSGDLVEAVVSQCDEREVYLDVGGKTEGACPIA
                     EFSEPPPVGSRVSVVLVSRGGDQPPRFSRRLAEQRKAWEDIKEAFQSKARLSGKIQRV
                     VDHGFIVNVGQLDLFMPLSQSGLRPNKNLSVGSEIDFKILEIKDNKHSAIVSHRAILE
                     EKNEALWNDLTSKHHEGDVVAAKVARRVSYGLFAELCGIEGLVHQSDVSYKKNVSLRD
                     RFPIGAEIQVKILKMDRENNRLSLGIKQLQEDPWEWAQRELKVGDVVDGTIMNVTNYG
                     AFFEIREGLEGLIHVSDLTWSGKRENPGKLVEPGKPARAKILSIDPANKRISLGLKQL
                     EEDPFEQVVRGLKVGDTAEGVVSSITKFGAFVKLRDGVDGLIRYSDYSHDEPVDKKML
                     TAGQTVTFKVLEIDRGKRKITLGMKQLSPGPYELLKKKYKRGDVLDCTVRNVTQFGLF
                     VSFGEKQEALIHISRIPIPEGKKLEDLFKQGDQLKAVLTRIDSDDKKISMSILDYEKK
                     TERDIMNQYLKKEDGPSTSSLGAYMKNLKL"
     gene            complement(8432..9112)
                     /gene="cmk"
                     /locus_tag="PLG78_04725"
     CDS             complement(8432..9112)
                     /gene="cmk"
                     /locus_tag="PLG78_04725"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNE22443.1"
                     /translation="MKSTVITLDGPAGSGKSSVAKELARRLGMLHADSGGIYRTFTLA
                     LMRRLGPCPSHEAFGEALSQSSISPESLEVSILTDGTHQYNLIRGQDQADEIRSPDVT
                     SRIRYVADRPVFREAVNALLRGMTNVVADGRDMGSVVFPNASYKYYLEASVDIRAARR
                     AAENEARGWKEDLGEIKESIRKRDEEDEKREYGALIRTPDMILIDTGPLNLEGVIGRI
                     LNSLQIKF"
     gene            complement(9109..9999)
                     /locus_tag="PLG78_04730"
     CDS             complement(9109..9999)
                     /locus_tag="PLG78_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNE22444.1"
                     /translation="MIRFKSVWIHGMGMMGASLALALRKSSQFEGRVFGAVRSQKSAD
                     YIRSTDLCDEVHVVSSGREAREHFDSLPESSLIVIGVPVASAVSLLGDLRGIQHLVTD
                     MSSTRAEVEKASAGVRFVGSHPICGSEDTGPQAARPGLFETRLCIITPSPESRNEDIQ
                     VVSDFWKDLGMNVMSMTAPEHDRILAYLSHAPHVISGLMAHWGMSDAVAEAVRKSPVP
                     LTGGGFRDMVRIAGSNPEMWLDIIRTNRREVIGSLETFQKYLADTIGLLKGDDEQKMM
                     EWLRSARLKRNRLCGYPDDK"
     gene            complement(9996..>10358)
                     /locus_tag="PLG78_04735"
     CDS             complement(9996..>10358)
                     /locus_tag="PLG78_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22445.1"
                     /translation="DAVFESLLDGDVLCYGEVVLRPDHRRYIILSTWNCPRSGRDKTS
                     VAASGDLKALISCIEDAGREVLRVETFHSQKHQGPCAFLDFPGHREDPDCAVILENIK
                     SRSDYFHFLGSYPAAAEL"
BASE COUNT         2617 a         2760 c         2477 g         2504 t
ORIGIN      
        1 cgacgacagg tcagccgtct gcgcaatcgc aagccttcag cgtaagcggg ggaccatatg
       61 gattggccat ggacccgacc aacacctacc tcttcgccgc aggtggaacc acgctgtatg
      121 tctttaagcg agattcggca ggcactttaa cacaaatcgc ccaaccgact cctggaggag
      181 ccgccttcaa caaagttgcg atctactcca ccagcaattt ctagtaaaga agtaccgcgt
      241 ccggcccgtg ttccgttgca accggatgca cgaaaacgcc cccgacccgg atgtaatgcg
      301 aacaaaacgt gcgtgataat tacaacgccg tgtgtattgc gtcatttctt tgcgtgataa
      361 aaccggagac gcgaaaacgc gcccggcccc ggatgtaatg caaacaaaac gtgcgtgatc
      421 attacaacgc gcgtgtgtat tgcgtcattt ctttgcgtga taaaaccgga ggcgcgaaaa
      481 cgcgcccggg gatgaaccat tgttacgcga tgactgtccg cgatttcatt tgcagtactc
      541 tggctgcaga ggaggcggtg cgcccggatc actgaagttt ggattggaaa gaaagcattc
      601 atctttcaaa gctttgagaa attccaccag gtctgctttg tcctgacgcg aaaggggaag
      661 agctcgaacg cgcacatctt tattcgggtt ctttgtaccg tctccagcaa agggaccatt
      721 cgcaatgttt cttccgccgt tcgcaaagac ttcaattgct tcctcaagag tcgcgacgct
      781 tccgtcgtgc atgtagggtg cagtcacaga gacattgcga agagagggtg tacgaaactt
      841 tccacgatcc tctttttttc cggttacaag agacaaccct tgagtctgcc gtgccgcagc
      901 tgaaccgtgc aaggagtggt cgggataatc tcccgcgtct gagacgttgt agagaccgac
      961 gttttgatac cagagctttc cggcctgatc gtcttgattg aaatcgcggc ctccgtggca
     1021 gtgaaaacaa cctgtcttct ccgactgaaa aagtgcatac ccgcgcttcg cagcatcatt
     1081 catgtggcca agatcgaagg gagactggta ggagatcagc gtagtttgaa agacggcaag
     1141 cgcccttgca atgtgactga tgtcgattgg ctcccccgga aacgcctgtt cgaacatcgc
     1201 ccgaacgttc tgatctacct tcaaccgatc gaggaccacg tactcttttc cgctcacgtt
     1261 cagttcctcg attgtcacat ccgatttctc ggaaaagaaa gggatcaaag tctgattttc
     1321 gaggcgagtg agttctggca atgtccacgt aaggaccgag aaataaccaa catctgccag
     1381 gcctggaaca ttgcgggggt gaatttgtcc agtggaacca gtgggtcgag ccttcccatc
     1441 cgcaaaacca agcgcggcta cgtgacaggt gctgcaggac tgtgtttcat tgagagacaa
     1501 ccttctgtca tagaaaagac tcctgccgag tgcgacacgc tctggtgtca tacaaacttc
     1561 tggaggttga gccggaggag aaacccctgg cggggttcca aaatcatact caggacatgt
     1621 tccactgagc gcataacgta gcttcgcaca gccggtagcg aaactgacga caaccagcag
     1681 ggaaaaaaga aatcgcatga gtggccaggc aaatgcgaaa cgcccggggc gacggggcac
     1741 gccttcgagc cttccagacc tcttcatcag aatccaggag gatcgctggt tggatgaaac
     1801 gtaacggttg tattgagacc gttgatggtg gcaagagtca gatcatcgta cgttccagcg
     1861 ccattcggag ctgtgttgat cttcccgaat actgtctgca cacccggata accaccggca
     1921 cctgcagatg gatcaggata accggttggc acagtggatg cgcttaaacc atcttgaatg
     1981 aaaccaccac cattaaagat tccaatcccc agaatatttg ccccgcacac agttccgcct
     2041 gtcgcaagac ttcccagcgc tacaccggat tgcgccgtgg tttcaaaatt acttgttgtt
     2101 ttttgaccat aagcaagtgc atagtaatgg tttccagtcc cacaaccagt ggtcatggat
     2161 acggtcctgg ccgcaatgcg aacgcacagt cccgtagcag ttccgccgtt cttgagccca
     2221 agagtcattt catccagaac cacacgtccc cggtcactgc tggacgccgc atttgcacct
     2281 gacagccaga actgcgctcc ccaggaatac gtctgatgtc tgctaggcga agcgcccgat
     2341 atataacttt ctacaatcaa attcgtgttg tcagaaacag tgggcgaaag aattcccaga
     2401 ttgtacgtca gcaggatacc agaagtagga ttggtcacat cattgaacat caactccagc
     2461 acatttgcat tgtcactggc gcgccacatt ttgaaacgat tggtaaaatt caggcccgac
     2521 gctgcagcag ccgtaacacc tgtgcaattc gcgccacagc caccggtctg cagctttacc
     2581 ttgtagacaa ctccgtttac agtagccgtg ctctgcaccg tcccagaatt cttgagaagt
     2641 cctgaattct tgagactcat gatcaggttg tctgcgagag tggaattact gtttgcccag
     2701 gttgcggatt gacgaacagc aaaccagtca ttcgcactcg ttgcgtgcaa cttctgagga
     2761 ccatccatta cgaacttcca tgcaggttgt gcagtagtga gatgaagagt tccagtttgc
     2821 agagtctgca aactcgaaac aatgggagag aaatcgggtg cagacaccgc aagccctgcc
     2881 gggtgttctt cctgagtgac gcgcagcgaa agccccagga ccaggaatgg aacgattgca
     2941 gaaaggagaa gaaaacgttt tgatttcaat ttggaactcc aggcggaatg caaatcacga
     3001 gccctgaatt attcttacac ccctggctat tcagataggc cgccccaaac aagagaagaa
     3061 gcagattggt gttatccttc tcctccttct tgtccagatt caagaaagca caggatccca
     3121 gtccaagcac acaaaccata agaatgattg ccatcttccg taccatactc acctcaatac
     3181 atctgacgta aaatcgacgc cgattgtatt tcccatcaag taaatttcta tgcaaaaaaa
     3241 agagtccctt gcgagacgtt aagccttttg cattaggaat gcattgaatg cccgcgccag
     3301 ctcgaaatct ttcagagtta gcccaccggc atcgtgtgta gtgaaacgaa catccacacg
     3361 attgtaacta ttcgaccagt ccgggtgatg atctgctttc tcgcatacaa gggcacagcc
     3421 cgacatgaac gagaatgctc tttgaaaatc agaaaatgca aatacccgtc gaatcgactc
     3481 cccttcccgg gcccaaccag gcgtatcctg caacatagat tcgatctggc tttccgaaag
     3541 cttttctctc actcgctcac gaaacacagc ttcacacacc gtggcgacaa attatcggtg
     3601 actcccggtc ctccggctgg acccttgccc gatgagccgc gcgctcctgt tgctcacact
     3661 gttcgccata tcctgcagct ccccctactt aagaaacagg ggaagagacg cactggatat
     3721 ctttaccgtg gaatttcaga ccagggccta tggagcgagt gttcgcgccg gcccggtcaa
     3781 agcaggtctc taccataaat cggctgacgg cgcagccgcc ggtttgcgcg gaggggatct
     3841 gggagttcat cacgatgcgg agttcgcggt cctatttttt ggcgcagact attttcagaa
     3901 aaagccgtta ggcgatctgg aaacaccttc ggaagaagac tcaaaaaccg cagaagaaac
     3961 cgatccgtcg cagacagcac tccgcggaaa agaattcaga gcacggtcac cctttggaac
     4021 cactcaaccc gcccagaaga agcgctcggt ctttaaaaag aaaggtgggt ttgcccctgc
     4081 ttcctactat acgcaactgg aagtctcact cggaatcttt tttggcgtga agctcggcat
     4141 caatcccgga gagcttttgg atgcgctcct cgggtggacc actctggaca tattcaggga
     4201 cgatgagcct tttgagacag aacaggaaaa ggcattgcga gacaatccgc tttacgacag
     4261 gctcacggac gaacaaaggc aaaagctcaa agagcaaatg gcaaagtgag acacctttgg
     4321 gtggttagtc gattggatca aattcgtgca tggcaatcga ctctgacctg aggcccgtgt
     4381 ccagattgta accatcgtat ttctcggcaa gatcgcgcaa cttctttgaa ccagtaatga
     4441 atgcgtctgc gtctgccatt gcatctgcgg caaacttcgc ggccctgtga accagatcca
     4501 tgaaactctc tttcgatgtg acctgtggat ctcctggata cttccattcc tttctctctt
     4561 cattgagtaa tcttggattg atctgatccg gagggggcaa gagcaaataa cgggctttga
     4621 ttgtgtgaaa ggttgcaaag tcgattgccg tgaggagcgc tcgcaccggg cgcgaacgag
     4681 tatccagaat gcgattgaac ttcatgaatc caacatacgc ttcgttgaga atgtcccctg
     4741 gaagaatgcg agcctctgag ccaaagtaag tattttcaaa ttcgtcgcgg taaaccatcg
     4801 caagagcatc cacccacatg gaccagagat catagtccat cgccggacca tccggagtct
     4861 cttcatggat gtccacatac tgtgcaaaat tgtaccggga tggttcgagt ccccagcgat
     4921 gatgcaccag atacgcgtcg agttgaaatt caacgcgaag atgattctcc tgagcgcgaa
     4981 ctgcctgttt gagatcgatg gagtagtagt ccccggtgat ataaaatatg tagggatgtg
     5041 ttacaatatc caccgcgcag tgcgaaatgt accccattgc aaaagcgtag catttttcgc
     5101 gacggatccc ttctggcata tctttgaccc tttgcagaaa cccgatcatc aaatcaaaca
     5161 cgcgagaatg atgagacaaa ttgccccagt gtagaccaag ctggttgcgt ttgcgcgaaa
     5221 gaacatgata gaagtaaaaa acatctggcc cgactgatcc aagagccgca aaattcattc
     5281 tcctgtgaac ctgttcggca catgccttat cgcgcgcaga tccatgctcc agcaaaacca
     5341 gagactggcg gagaatttcc agatgtgtga tctttgcagc cagaagatta tccaggcagg
     5401 tgactgcgaa aacggctgag catcccctgc actttctgtg attcaagagc gtaagcgaat
     5461 cttcctacaa ccaggaccgc ttcgctattc aaaatgactt cttgctccac aagaccattg
     5521 gcgcgaagag tcgatccggt gctcacaaga tccacaatgc aatccgcaag acccagagcg
     5581 ggcgcaattt caacggaacc atgaagcttt atgatttcgc acgaaattcc tttctgatat
     5641 aggaagttca ttgcctgcac tggatacttg gtcgcgacac gaagatgctt tttttcaaga
     5701 ggtttttcgt ccggctttcc cgcaacagac agtctgcatt tcccaaaacc aagctcgagt
     5761 ggtgtagtca catcaaacgt gctttcgagg agtacatctt ttccagtaat tccagcgtgg
     5821 gccccgccct gaatgatgta ggtaggaaca tctgtgggcc gaaccagaat gattcgatat
     5881 tccccggatg gatccgtgaa gcacagttcg cgcgaccctt ctggaatctg aatgtcgcat
     5941 agaccgctgg aacgaaaaaa ttgaatgctt tcgagcgcaa gtcgtcccgc tggcagagcg
     6001 aatgtgaaaa cgcccatatt actttccgtg ttgaatcaaa aatgccgcgg ccgcaatgcg
     6061 aagatcagca atgctttcta catctttgat ctcgagcatt tttttcatga cttcgcggcc
     6121 ctcgcggtct ttgttcagca gaatcaatgc acgcgcctgg ccaaatctcg caagagcagc
     6181 agctccgcct tcgtccataa gcttctgcga tgcattggaa taggcagact ctgccttcgc
     6241 aaacttgcct ttctcttcaa acaaaagacc cgctctgtag gaaaacagcg cagaaagctc
     6301 gggcgtttcc acgatgcgcg caatttcaac aagcgcgtcc cctgctttct ctttttcgcc
     6361 tgtcttgata aggagatcta cctttttgag acctgcgcga agctgggcgt gatcgtttgg
     6421 aaattcgcgc tcatacttct caagcttttc aatggcaacc ttttcagcgc cactgcctga
     6481 tgccatcaca gggcttttca tcagttcctc aaaatcagtg aggctctttt gctcgcgata
     6541 atcccggtaa atgaagaatg caaggctggc agtcccgacg accacaacca gaatcaagcc
     6601 aagcacaacc tgtcttgaat gctgacgcat ccagtaaaat gccgtagcaa cagcgcgctc
     6661 aaactgattc ccgtggaaat cgcgtaacgg atcaactgct tctggagtaa tgacttttct
     6721 acgcatatca gagcttcagg tttttcatgt aagcacccag actgctcgta gacgggccgt
     6781 cctctttttt caggtactga ttcatgatgt ctctttctgt cttcttctca tagtcgagga
     6841 tagacatgga aatctttttg tcgtcggaat caattcgtgt gagcactgct ttgagctggt
     6901 ctccctgctt gaacaggtct tcaagcttct tgccctctgg aattggaatg cgcgatatgt
     6961 gaatcagcgc ctcttgcttt tctccaaagg agacaaacag accaaattga gtcacattgc
     7021 gaacagtgca atcgaggaca tctccacgtt tgtatttctt tttgagaagt tcatacgggc
     7081 ctggcgaaag ttgcttcatt cccagagtga tcttgcgctt gccgcgatca atttcgagaa
     7141 ccttgaaggt gacagtttgc cctgctgtga gcatcttctt gtctacaggc tcatcatggg
     7201 aataatccga atatcgaatg agtccgtcca cgccatcgcg taacttcacg aaggcgccaa
     7261 acttggttat gctggaaact acaccttctg ctgtgtctcc tactttcaga ccacgaacca
     7321 cctgttcaaa tggatcttct tccagttgct tgaggccaag tgagattctc ttatttgcag
     7381 gatcaatcga gagtattttc gctctggctg gctttccagg ttcaaccagt ttgcctggat
     7441 tttcgcgctt accggaccat gtaagatccg aaacatgaat gagaccctcc aggccctcgc
     7501 gaatttcaaa aaatgcaccg taatttgtga cgttcatgat ggttccatcc accacatcgc
     7561 ctactttcag ttctctttgc gcccattccc agggatcttc ctgcaactgc ttgattccta
     7621 gagagagtct gttgttttcg cggtccatct tcaggatctt aacctgaatt tctgcaccga
     7681 taggaaagcg atcgcgaaga gaaacattct tcttgtaaga aacatctgac tgatgaacga
     7741 gcccttctat gccgcagagc tccgcgaaga gtccatagga aaccctgcgc gccactttcg
     7801 cggcaacaac atcaccttcg tggtgtttgc tggtgagatc attccagagt gcttcattct
     7861 tctcttcaag aatggcacgg tggcttacaa ttgcggaatg cttgttgtct ttgatttcta
     7921 aaatcttgaa atcaatctca gaaccaacag agaggttctt gttcggacgc aggcccgact
     7981 gagacagagg catgaaaaga tcgagctggc ctacgtttac aatgaatcca tgatctacga
     8041 cgcgctgaat ttttccgctc agacgcgctt tggactgaaa cgcttcttta atatcttccc
     8101 aggcctttct ttgttccgcg agtctgcggg aaaaacgagg aggctgatct ccaccgcgtg
     8161 atacgagcac cacggatacg cgagatccaa caggaggtgg ttctgagaac tcagcaatcg
     8221 gacaggcgcc ttccgttttt ccgccaacgt ccagataaac ttcacgttca tcgcactggc
     8281 tgacaactgc ttctaccaga tccccgcttt gaacatctcc ctggccgtct agcgcgccaa
     8341 gcatgtcctc catcgactta atctctttct gatccaggtc aaagggtgtc gtgcttttcg
     8401 atgtcatgaa aatggaattg tgagaggagg tttaaaactt aatctgcaga ctgttgagta
     8461 tgcgtccgat aacaccttcc aaatttagag ggccggtgtc aattagaatc atatctggag
     8521 ttcgtatcaa tgccccgtac tcgcgttttt catcttcttc atcgcgttta cggatgcttt
     8581 ctttgatttc gcccagatct tccttccaac cgcgggcctc attctcagcc gctctgcgcg
     8641 cagccctgat gtcaacggac gcttccagat aatacttata cgatgcattt ggaaaaacta
     8701 cactgcccat gtcgcggccg tcggccacga cgttggtcat cccgcgcaaa agagcattca
     8761 ccgcctcacg aaagaccggg cgatcggcca cgtatcgaat ccgggaagta acatctggag
     8821 aacgaatctc gtctgcctga tcctgaccgc gaatcagatt gtactgatgt gttccatccg
     8881 taagtatact gacttcgaga gattccgggc ttatggacga ttgagaaagt gcctctccga
     8941 atgcctcatg cgaaggacac ggccccagtc ttctcatcaa tgcaagtgta aacgttctat
     9001 aaattccgcc ggagtccgcg tgcaacatac ccagacgacg cgcaagctcc ttggcaacgc
     9061 tactcttccc tgaaccggca gggccatcca gtgtgatgac tgttgatttc atttgtcgtc
     9121 aggatatccg caaagacgat ttcttttgag tcgagcagaa cgcagccatt ccatcatctt
     9181 ctgttcgtca tcgcctttta agagtccaat tgtgtccgcg agatattttt gaaatgtttc
     9241 gagcgacccg atcacttcgc ggcgatttgt gcgaatgata tccagccaca tttctggatt
     9301 gcttccagcg atccggacca tgtctctaaa gccgccgcca gttaatggca ccggagattt
     9361 tcgcacggct tccgctacag cgtctgacat gccccagtga gccatcaggc cggaaatcac
     9421 atgaggggcg tgcgaaaggt aggcgagtat gcgatcatgc tccggcgcag tcatggacat
     9481 gacattcata cccaggtcct tccagaaatc gctcactact tgaatgtctt cgtttctgga
     9541 ctcaggagaa ggagtgatga tgcagagtct ggtttcaaac aatccaggac gcgcagcttg
     9601 aggtccggta tcttctgaac cgcagattgg atgagatcca acaaatcgca caccggcaga
     9661 tgccttttcc acttccgccc tcgtagatga catgtctgtc accagatgtt ggattccgcg
     9721 aagatctcct agaagggaaa ccgcagaggc aacaggcaca ccaattacaa tgagagaaga
     9781 ttctggcaga gaatcaaaat gctcacgggc ttctctccca cttgatacaa cgtgcacttc
     9841 atcgcataaa tctgtagaac gaatgtaatc cgcgcttttc tgactgcgaa ccgcgccgaa
     9901 cacgcgacct tcgaattgcg aagacttgcg aagggcgagc gcaagagaag cgcccatcat
     9961 gcccatgccg tgaatccata cggacttaaa acgaatcaaa gctctgccgc cgcaggatag
    10021 gacccaagaa agtgaaagta gtccgaacga gatttgatgt tttcgagaat cacagcacag
    10081 tcagggtcct ctctgtgacc gggaaagtca agaaacgcgc acgggccctg gtgtttctgc
    10141 gaatgaaacg tttccactcg caaaacttct ctccccgcat cttcaatgca gctgatgagt
    10201 gctttcaaat ctccgctggc ggcaacgctt gttttatctc gtccagatcg cggacagttc
    10261 cacgtggaaa gaatgatgta tcttctgtga tccgggcgaa gaaccacttc gccatagcaa
    10321 agcacatctc catcgagcag ggattcaaag accgcgtc
//
LOCUS       DAOQJL010000069        10356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3359_length_10356_cov_4.431803, whole genome shotgun sequence.
ACCESSION   DAOQJL010000069 DAOQJL010000000
VERSION     DAOQJL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_10356_cov_4.431803"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLG78_04740"
     CDS             complement(<1..866)
                     /locus_tag="PLG78_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNE22446.1"
                     /translation="MTELFTEKEITVQDALEHGLTADEFERIRGFLGRLPTVTELGIY
                     SGLWSEHCSYKNSILQLKTLPTESPRLAAKTGEENAGALDIGDGLAVVFKIESHNHPT
                     AVEPYQGAATGVGGIMRDIFTMGARPICSLNSLRFGYPSEKRSRYLLSRAVKGIGDYG
                     NCLGVPVSGGEIFFDESFTRNCLVNAMTVGIVKKDKMVKAKAEGEGNPVFYVGARTGR
                     DGIHGASFASRDLSEESESKRSAVQVGDPFMEKLLMEASLELAQTDSFIGMQDMGAAG
                     LSCATSEMSAKGA"
     gene            complement(859..2430)
                     /locus_tag="PLG78_04745"
     CDS             complement(859..2430)
                     /locus_tag="PLG78_04745"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNE22447.1"
                     /translation="MNLQIFAIGSIYICALIRACPLFLLGPETGKLTATVVEKIAFVL
                     SVILAIMLIFKPPRKTETTERSYAENVLAIVLISTFTLTGILFSIRCMDGLILGAYDY
                     TGISLVAGGHLYGQFLSSPYYQSANGSIFYLAHHFSPILAFYSPFYYLTSSHAIYGIL
                     LQITAAIGMYAAYRFARAGLLAACAIVFLPVIYQFTISFHYEIFVLWFFFLLLGGIRK
                     KQWVQISLGLLLLLMVKEDMSVYLSLFAVYLITGKDRVERKIGAVILAVCAVSFVCVS
                     ILYMPRASGEVNSRFLTYWGVSSLSDFIFSLTPTRVAGAWIDSWPVIISFIAQTAFLP
                     LLRPRLSLLVLVPIFTLHLLSSQPFFHTLSAYYMYTFTPFLLYGAFEGAKTFKHFRSA
                     AIAGFCAAAAFTASTRKEVPMVVLPDPSAGPEIRALLSKMEPGRKLWTHAFLSAQAPV
                     QNPVFPLLHPTAPDYILIDPDRSSPQGESPQDVEKALQIARRGCIVGRASGTVLYRVS
                     QELTQETKEKPCTRD"
     gene            complement(2449..3465)
                     /locus_tag="PLG78_04750"
     CDS             complement(2449..3465)
                     /locus_tag="PLG78_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE22448.1"
                     /translation="MKKRRLGTSSMVVSEIGLGTMTFGSSCDEETAFKIMDLAYESGI
                     DFFDTAEIYPVPPEAKWVHRTEEIVGKWMKGKPRDSIILATKVCGPGHGWFVPPVRSG
                     KTALDRVNIRRAIEGSLERLQTDYIDLYQTHWPDHDMRYEDTLEVLTELEEEGLIRAA
                     GCSNETAWGLMKALQVSEYHGLIKYRTIQNNFSMVNRRFEDALAQVCRKEQVSLLPYS
                     PLAGGVLSGKYNDNQVPKNARFTKYLESGERQKKQAHRFVNDKTLSSTAAFHKIAAEA
                     GIHPIAMAVAWSKQHDFVASTLIGANTPEQLREILGGDELILSPEILAKIDEVSKSIM
                     YPMN"
     gene            complement(3505..4833)
                     /locus_tag="PLG78_04755"
     CDS             complement(3505..4833)
                     /locus_tag="PLG78_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE22449.1"
                     /translation="MIALPTKRAAILFAVPLVLSVFLLPYFILSGSNLPAAPIILGMN
                     LFFLGIFAMDWFTIPRAKRFTAERETEPVLLQNAKHSITLALIYHLKKGASVQSSIYD
                     DILPFMECKTFPLAVPISPGRNEFSYPVTVSRRGPYDLKHVHLTVFGALGLARKVYRI
                     PCETSLRVYPDLRSVSKLILLSRTSRLSFMGIRRIRTTGGDTEFERLREYTRDDEFRR
                     IDWKATARQRRFMVRQYQQSRNQTIIFMLDCGRMMTAESQGVRVLDQAISSILLLARV
                     ALDQGDRVGILAFSSRVLRFVAPVSGSGKHRVLVQACYDLYASHEETNFDLAFRHLQR
                     ASRKRALVCVVTNVIDDANADMIRSHLEVLSGRHLPFAVLLKIREIHEMAEKANDAAI
                     RLGVSDFLEWRKNVIHRLSAGGVLVLESFPDQLEVSLINQYLWIKARNLL"
     gene            complement(4830..5762)
                     /locus_tag="PLG78_04760"
     CDS             complement(4830..5762)
                     /locus_tag="PLG78_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE22450.1"
                     /translation="MTPEIQNLRREIGKVFVGQEDLVTGVLTCLLGGGHALLEGMPGL
                     GKTLLVRTLGGVLGLDFARIQFTPDLMPTDVTGTQIYNQKNQEFVFHRGPVFTHILLA
                     DEINRSPAKTHSALLEIMQEQQITLDNQTYKIDAPFLVLATQNPIESEGTYALPEAAL
                     DRFLLKLKINYPGEQEELEILRLQTRPSVEQAIARVITRDQVLELQNKTAQIHVANSV
                     LTYINSLVRATRQRREIYVGASPRAGIALLKTARAVALLAGRTYVVPDDVKKMAHSAM
                     RHRLLLLPEVEVEGKTPDDIIDDLLATVPVPKDE"
     gene            complement(5759..6955)
                     /locus_tag="PLG78_04765"
     CDS             complement(5759..6955)
                     /locus_tag="PLG78_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22451.1"
                     /translation="MTHRFILTLLVFATFCRGPLVEEDYASIGGKSINGIDSFAEILK
                     STGRTVRTSAYFTKETATSDVIVHFEPDFGYDEEVYRNLEAWMSGLDLNPEDLTDKDK
                     VVHDSRTYAVPDTESKEAHGDPAEKTDEEETADNTNTEKEVDLKPVTLIYFLRDTTAS
                     AEFWRKLENALKDYPEERAFARYNLERTEFRESPPQGEIPTLFGFRSLLKGQKEVHQA
                     RSFSSDEIEDLPPWFPYRILHSGEPALSIEERFAYRTILLGDDHDLIREFFLKRGRVI
                     LVYNSESFLNYAQAKTAQRKFAEKFTTYALKDAPKDGTISIIQKAPVPPRTQKEKEES
                     PFRIFTVFPLNVIFFQLLVFLLVFLLSRAKVGAPLREEAPAGSRDFTEHFTALGNLIR
                     KTGTRS"
     gene            complement(6955..7755)
                     /locus_tag="PLG78_04770"
     CDS             complement(6955..7755)
                     /locus_tag="PLG78_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22452.1"
                     /translation="MYVSECFQNLAIEERTRLEKRLGIILRRVEERHQIRKTLRNIFE
                     SMEVRLRAEFEAGAIWTGLLDHFCILPQDRMTGEAAEALKKHPLTFWPESHICTMSAE
                     AMEVVGRMDCVRESRSILSELLRMGTPDKRALSRWLGLDAPASALDLYSLIGQTRGSE
                     SPDFSEFNHRYLTDLFPEDAMALLWFYRGVLPLYECLRLAETENIQAEQKKCLEGFRL
                     GYLIAEPLPVEFGQTQKFKIVCTREKNAGMVSDLPSLYKEDTLQRSLF"
     gene            complement(7765..9030)
                     /gene="purD"
                     /locus_tag="PLG78_04775"
     CDS             complement(7765..9030)
                     /gene="purD"
                     /locus_tag="PLG78_04775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNE22453.1"
                     /translation="MNVLLIGSGGREHALFWKLSQSTQLGSIRVFPGNGGFAPESIAL
                     GIDLKNFETIRSYVKQNKIDLVVVGPEQPLVDGITDALSRDCLVFGPTAFCAQLEGSK
                     DFSKAFMKRHGIPTADAVTFTDSNAAIEYLRTRKLPIVVKADGLAAGKGVTVATSYED
                     AETAVRDAIDKKIFGTSGQSVLIEDFLEGQEASVFAICDGQRALPMIAAQDHKRVFDN
                     DQGPNTGGMGAYAPVPFMNADVMRQVQTEVLDRVMAGMQKEGHPYRGLLYAGLMVHNG
                     SVRVVEFNCRFGDPETQGLLRLLDEDLLELCKLSASGNLPERQIHFKQEAVICIVLAA
                     EGYPGEVRKNIPIPDLKIIDSLGDIIMFHAGTALTPGGVVSTGGRVLNLTATGRDLLE
                     ARKLAYSALERVHVPGLFYRKDIGQKGIP"
     gene            complement(9032..9919)
                     /locus_tag="PLG78_04780"
     CDS             complement(9032..9919)
                     /locus_tag="PLG78_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE22454.1"
                     /translation="MNAAAWKEGGKYFRHNGHNIFYRLDGSGEAVIFLHGFPTSSWDW
                     EKIWPALTSQFLCVAPDFIGYGYSDKPHEYNYSILDQADLAENLLRELRIEKAHIFAH
                     DYGVSIAQEMLARSQDRKRERIKGGLEILSVCFLNGGIFPEAHRPRLIQQLLLSPVGR
                     WVSMFINEGAFRASFCEIFGKGTQPAEEDLKQFWELIRLNKGHEIYYRLISYIPEREK
                     NRERWVNAIMNSRVPLRLINGPEDPVSGKHMADQYKKLIQKPDVVLLPGIGHYPQYEN
                     PDAVIANFLEFVRRKEEEE"
     gene            complement(10000..10335)
                     /locus_tag="PLG78_04785"
     CDS             complement(10000..10335)
                     /locus_tag="PLG78_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNE22455.1"
                     /translation="MTTVLLLIASNVFMTFAWYGHLKFRHIPIWAAIFASWGIAFFEY
                     CLQVPANRLGYGQFNAFQLKIIQEIVTLVVFIVFAIFYLGESPRWNHLASLLCIVMAV
                     YFAFLSAPA"
BASE COUNT         2519 a         2514 c         2600 g         2723 t
ORIGIN      
        1 gcacctttcg cactcatctc tgatgtggcg caggaaagtc cggccgcgcc catatcctgc
       61 attccaatga aggaatcagt ctgcgcaagc tcgagagacg cctccataag aagcttctcc
      121 atgaatggat ctcccacctg gactgcgctg cgtttgcttt ccgattcttc tgaaaggtcg
      181 cggcttgcga aactggcccc gtggattcca tctctgcctg tgcgtgcgcc cacatagaaa
      241 acagggttgc cttctccctc tgcttttgcc tttaccattt tgtctttctt aacaatacca
      301 acggtcatcg cgtttacgag acagttacgt gtgaaagact catcaaagaa gatttctccg
      361 cctgataccg gaactccaag gcagtttcca tagtctccga ttcctttcac tgctctactg
      421 agcagataac ggctgcgttt ttctgacgga taaccgaagc gaagagagtt gagggaacag
      481 atgggtctgg cacccatagt gaagatatcg cgcatgattc caccaacacc tgtggccgcg
      541 ccctggtaag gctccactgc agttgggtga ttgtgacttt cgattttgaa aacgactgca
      601 agtccatctc cgatgtccag tgcgcctgcg ttctcttctc cggtttttgc cgcaagcctg
      661 ggcgattcgg tcggcagagt cttcagttgt agtatggaat tcttatagct gcaatgttct
      721 gaccagagtc ctgagtagat tccaagttca gttactgtgg gcagccttcc gaggaagccg
      781 cgaattcttt cgaattcatc tgcagtcagc ccgtgttcca atgcatcctg gacggtgatt
      841 tctttttctg tgaataattc agtcacgggt gcatggtttt tcttttgtct cctgtgtcaa
      901 ttcctgagac acacggtaga gaacggttcc ggaggctcgc cccacgatgc atcctctgcg
      961 agcaatctgg agagccttct ccacatcttg cggtgattcg ccttgcggcg agctgcgatc
     1021 tggatcaatc aaaatgtaat ccggggccgt cgggtgaaga aggggaaaaa ctggattttg
     1081 cacgggcgcc tgtgcagaga gaaaggcatg cgtccatagc tttcgccctg gttccatttt
     1141 tgaaagaagt gcgcgaattt cagggcctgc ggaggggtcc ggcaggacca ccatgggcac
     1201 ttcctttcgc gtggaagcag tgaaggctgc agccgcacaa aagccagcga tcgcagcaga
     1261 gcgaaaatgt ttgaaagttt tagcgccttc gaatgctcca tacagtagaa acggcgtaaa
     1321 cgtatacata tagtacgcgc tgagggtgtg aaagaatggt tgcgagctga gaagatggag
     1381 agtgaaaata ggaaccagaa cgaggagaga gagacgaggg cgcagaagag gcaagaaagc
     1441 tgtttgcgca ataaaagaaa taatcactgg ccaggagtcg atccaggctc ctgccactcg
     1501 cgttggagtc agggaaaaaa taaaatcaga taaagaagaa acgccccagt aagttaagaa
     1561 tctggagttt acttctccgg aagctctggg catataaagt atagaaacac aaacaaatga
     1621 aaccgcgcat acggccagaa tcacagctcc gatctttctc tcgactctgt ccttcccggt
     1681 gatcaggtaa actgcaaaga gtgaaagata aacactcatg tcttctttca ccatgagtag
     1741 aagcaatagt ccaagagata tctgaaccca ctgtttcttc ctgattccgc cgagcagtaa
     1801 aaagaaaaac cagagaacaa atatctcgta gtgaaatgaa attgtgaact gatagatgac
     1861 aggaagaaat acaatcgcac acgcggccag cagtcccgcg cgcgcaaacc gataggcggc
     1921 atacattcca atggcggctg taatttgaag cagaatcccg tagatcgcat gcgaagaggt
     1981 cagataatag aagggagaat agaatgcgag tatgggtgaa aaatgatgcg cgaggtaaaa
     2041 gatggagccg tttgcactct ggtagtaggg ggaagaaaga aactgcccat agagatgccc
     2101 gcctgcaaca aggctgattc cagtgtagtc gtaggccccc agaatcaaac catccatgca
     2161 tcgaatcgaa aacaaaattc cggtaagtgt gaatgttgaa ataagaacga tcgcgaggac
     2221 gttttctgcg tacgaacgct ctgtggtctc cgtctttcgc ggcggtttga agatcagcat
     2281 gattgcaagg atcacagaga gtacgaaggc aatcttctca acaaccgttg cagtcagctt
     2341 tccggtctcc ggtccaagaa ggaacagagg acaggctcga atcaacgcgc agatataaat
     2401 ggagccaatg gcaaagattt gcagattcac ccgagaacgc cttcaaaatc aattcatggg
     2461 gtacatgatc gatttgctca cttcatcaat ttttgcaagt atttccggag atagaatcaa
     2521 ttcgtctcca ccgagaatct cgcgaagttg ttcaggagta ttggctccaa tcaaagtgga
     2581 agcaacaaaa tcatgctgct tgctccatgc tacagccatg gcgatgggat gaatgcccgc
     2641 ttccgctgcg attttatgaa atgccgcggt tgagctgagc gttttatcat tcacgaaccg
     2701 atgcgcctgt ttcttttgtc tttcacccga ttccagatac tttgtgaatc gtgcgttttt
     2761 tggcacttga ttgtcgttgt attttccgct gagcactccc cctgccagag gagaataggg
     2821 taatagactc acctgttcct tgcgacaaac ctgggccagt gcatcttcga atcttctgtt
     2881 caccatactg aagttgttct ggatggtgcg atatttgatc agtccgtggt actcggagac
     2941 ctgcaaagct ttcatcaatc cccacgcagt ttcgttgctg caaccggctg cgcgaatcaa
     3001 cccttcttct tctagctccg tgagtacttc tagagtatct tcgtatctca tatcatgatc
     3061 cggccagtga gtctggtaga gatcaatgta gtctgtttgc agtctttcga gacttccttc
     3121 gatggcgcgc cgaatgttga cacgatcgag agcagttttg ccggatctta ccggaggaac
     3181 aaaccatcca tggccgggcc cgcacacctt ggttgccaga atgatggaat cgcgaggttt
     3241 tcccttcatc cacttgccta cgatttcctc tgtgcgatga acccatttcg cttccggagg
     3301 tacagggtag atttcagccg tatcaaagaa atcaattccg gattcgtacg ccaggtccat
     3361 gattttgaat gccgtctctt catcgcagga agatccaaat gtcatggttc caaggccaat
     3421 ttcagaaact accatggagc ttgtgccgag tctgcgcttt ttcataaaga attcctattg
     3481 tttaaaataa ttagtatttc gtcgctacag cagatttctt gctttgatcc ataagtattg
     3541 attgatcaaa gacacttcca gctgatctgg aaagctttcc agaaccagaa cgcctcccgc
     3601 cgaaaggcga tgaatgacgt tctttctcca ctccagaaaa tctgaaacac caagacgaat
     3661 ggctgcatcg tttgcttttt ctgccatttc atgaatttcc cggattttca agagaactgc
     3721 aaatggaaga tgtctgccgc tgagtacttc caggtgagat cgaatcatgt ctgcatttgc
     3781 atcgtcgatt acatttgtga ccacgcaaac aagcgctctt ttgcgggatg cgcgctgcag
     3841 gtgacgaaac gcaagatcga aattggtttc ctcatgggac gcatacagat cgtaacaggc
     3901 ctgcaccaga acgcgatgtt tgccggagcc ggaaacaggc gcaacaaagc gaagaacgcg
     3961 cgaactaaaa gcgagaattc ccactctgtc tccctgatcc agggccactc gcgcaagcag
     4021 aagtatactc gagattgcct gatcgagcac gcgcactccc tgggattcgg cagtcatcat
     4081 tcgcccgcag tccagcatga agatgatggt ttgatttctg ctctgctggt attgacgcac
     4141 catgaagcgt ctttgtctcg cggttgcttt ccagtcgatt ctgcgaaatt catcatctcg
     4201 cgtatattcg cgcagccttt cgaattcagt atctccacct gttgtgcgaa tgcggcggat
     4261 gcccatgaag ctcaatctgg atgtgcgcga aagtagaatg agcttgctta cacttctaag
     4321 atcggggtat acacggaggc tcgtttcgca tggaatgcga tacactttgc gcgcgagccc
     4381 aagtgctccg aagactgtaa ggtgaacgtg ctttagatcg tacgggccac gcctgcttac
     4441 agttacagga tacgaaaatt catttcttcc tggagagatg ggcacggcca gaggaaatgt
     4501 tttgcattcc atgaaaggaa gaatgtcgtc gtaaatggat gattgaacgg acgcgccttt
     4561 ttttaaatgg tagatgagtg caagtgtgat cgagtgcttt gcattctgca agagaactgg
     4621 ttctgtttcg cgttccgccg tgaatcgctt cgcgcgtgga atggtaaacc agtccatggc
     4681 gaagattcca aggaagaaca aattcatccc cagaatgatg ggcgctgcag gcagattgct
     4741 tcctgaaagg atgaaatacg gaagcaaaaa tacggacaga accagcggga ctgcaaacag
     4801 aatcgccgcg cgttttgttg gcagcgcaat cattcgtctt ttggcaccgg cacggttgcg
     4861 agtagatcgt caatgatgtc atccggcgtt ttgccttcta cttccacttc cggtaaaaga
     4921 agcaatctat gacgcatggc ggaatgagcc attttcttaa cgtcatccgg cactacatag
     4981 gttctgccgg ccagcagtgc cacggcgcgt gctgttttca aaagtgcaat gcctgcgcga
     5041 ggagaagcac ccacatagat ttcacgacgt tgcctggtgg cgcgcacgag tgaattgatg
     5101 tatgtgagta ctgagttggc cacgtggatc tgtgctgtct tgttttgaag ttccagcacc
     5161 tggtcgcgag tgatcacgcg cgcaatagct tgctctacgg aaggtctggt ttgcagacga
     5221 aggatttcaa gttcttcctg ttcacctgga taattgattt tcaatttgag aagaaagcgg
     5281 tcgagtgctg cttctggcag cgcgtaggtt ccttctgatt caatcggatt ctgcgttgcc
     5341 agtacaagaa agggcgcgtc tattttgtat gtttgattgt ctagagtgat ctgctgttct
     5401 tgcatgattt caagaagtgc agagtgcgtt tttgcgggag atcggttgat ttcatctgca
     5461 agtagaatgt gagtgaacac aggcccgcga tggaaaacaa attcctggtt cttctgattg
     5521 taaatttgag tgcccgttac gtccgttggc ataaggtcag gagtgaactg gatgcgtgcg
     5581 aaatcgagcc cgagcacacc gcccagagtc cgaaccagca gtgttttccc gagacccggc
     5641 atcccttcta aaagagcatg ccctcctccg agcagacacg taaggactcc ggtgacaaga
     5701 tcctcctgtc ccacaaatac tttgccgatc tctcttctga ggttctggat ttccggtgtc
     5761 atgatctggt tcctgttttg cgaatcaagt ttccaagtgc agtaaaatgt tctgtgaagt
     5821 ctctactccc cgctggcgct tcttcgcgca agggagcgcc aaccttcgcc ctggagagaa
     5881 ggaataccag gaggaagacg agcaactgaa aaaatatgac gttgagcggg aaaacagtga
     5941 agattctaaa cggactttct tccttttcct tttgagttcg cggcgggacg ggtgctttct
     6001 ggataatcga aatggttccg tctttaggag cgtctttgag agcgtaggtg gtgaattttt
     6061 cggcaaattt tctctgggcc gtttttgcct gcgcgtaatt aaggaaagat tcagaattgt
     6121 agactagaat cacgcgacct cttttgagaa agaattctcg tatgagatca tgatcgtctc
     6181 cgaggaggat ggtgcgataa gcgaaccttt cctctatgga aagagcaggt tctcccgagt
     6241 gaaggattct gtatggaaac cagggaggta ggtcttcgat ttcatcgctg ctaaaagatc
     6301 ttgcctgatg gacttctttc tggcctttca aaagggaacg aaagccgaat aacgttggaa
     6361 tttctccttg aggagggctc tcgcgaaact ctgttctttc cagattgtag cgtgcgaatg
     6421 ccctttcttc tggatagtct ttcagcgcgt tttccagctt tcgccagaat tcagcagaag
     6481 cggtcgtatc gcgcaagaag tagatcaacg tcacaggttt gagatcgact tccttttctg
     6541 tgtttgtatt gtctgcggtt tcttcttcgt ctgtcttctc tgcgggatcg ccgtgcgctt
     6601 ctttggattc tgtatctggc accgcgtacg tgcgagaatc atgaaccacc ttgtctttgt
     6661 ccgtgaggtc ttccggattc aaatccagac cggacatcca ggcttccagg ttgcgatata
     6721 cttcttcgtc gtaaccgaag tctggttcga aatgaacgat tacatctgat gttgcggttt
     6781 cttttgtaaa ataggcagac gtgcgaacgg ttcgtcccgt ggattttaag atttctgcga
     6841 agctgtctat gccgttgata ctcttgccac caatggatgc ataatcctct tctacaagag
     6901 gaccccggca gaatgtagca aagaccagta gtgtaagaat gaatctgtga gtcatcaaaa
     6961 gaggctacgc tgcagcgtat cctctttgta gagggaaggc aggtccgata ccatgccagc
     7021 atttttttct ctggtgcaca cgatcttgaa cttctgagtc tggccaaatt ccactggcag
     7081 tggctctgcg atcagatacc ccaggcgaaa tccttcgagg catttctttt gttctgcctg
     7141 gatgttttct gtttcagcga gtctcaggca ttcgtaaaga ggcaatactc ccctgtagaa
     7201 ccacaaaagg gccatggcat cttcggggaa caggtcggtg aggtaccgat gattgaattc
     7261 ggaaaagtct ggtgactcgc ttcctcttgt ctggccaatg agagagtata gatcgagtgc
     7321 ggaggcagga gcgtccaggc cgagccatcg cgagagtgcg cgcttgtccg gtgtgcccat
     7381 gcgcaatagt tctgacagga tggatctgct ttcgcgaacg caatccattc tacccactac
     7441 ttccatggct tctgcagaca tggtgcagat gtgcgattct ggccagaaag tgagaggatg
     7501 tttcttcaga gcttccgccg cttctccagt cattctatcc tggggcagaa tgcagaaatg
     7561 gtcgagcaat ccggtccaga tggcacctgc ttcgaattcg gcacgaaggc gcacttccat
     7621 ggattcaaaa atattgcgca gcgtcttacg gatctggtgt ctttcttcta ctcgcctgag
     7681 aatgatgccg agtctttttt caaggcgagt tctttcttct atcgcaaggt tctgaaagca
     7741 ttcggaaacg tacatcgcga cctctcaggg aattcccttc tgtcctatgt cctttcggta
     7801 gaagaggcct ggaacgtgaa cacgctccag ggcactgtat gcaagcttcc gcgcttcgag
     7861 caaatcacgg cccgttgcag tcagattcaa cactcttccc ccggtggaaa cgactccgcc
     7921 cggtgtaagg gcagtgccag cgtggaacat gattatgtct cctaacgagt caatgatttt
     7981 gagatccggg atgggtatat ttttccgcac ttcaccgggg taaccctctg ctgcgagcac
     8041 tatacagatg acggcttcct gtttgaaatg aatttgtctt tccggaagat ttccagaagc
     8101 actcagctta cacagttcaa gcaaatcttc atccagcaat ctgagaagcc cctgcgtttc
     8161 tggatcacca aatctgcagt tgaactcaac tactcgcaca gacccgttat gcaccatgag
     8221 gccagcatac aaaagtccgc gatacggatg accttctttc tgcattcccg ccatcacgcg
     8281 atcgagcact tccgtctgta cttgtcgcat aacgtctgcg ttcatgaaag gcacaggagc
     8341 atacgcgccc attccgcctg tgttcgggcc ctgatcattg tcgaatactc tcttgtggtc
     8401 ctgcgctgcg atcatgggta gagctctttg tccatcacag attgcgaaga cactcgcttc
     8461 ctgaccttct agaaagtctt ctatgagtac ggactgaccg gatgttccga atatcttttt
     8521 gtcgattgcg tctcttacgg ctgtttctgc atcttcgtag ctcgtggcca ccgttacgcc
     8581 tttgcctgca gccagaccat ccgctttcac tacgataggt agctttcgcg tgcgcagata
     8641 ctctatagct gcatttgagt ctgtgaatgt caccgcatcc gcagtcggga tgccgtgtct
     8701 tttcatgaac gcttttgaaa aatctttaga tccctctaat tgcgcgcaga aagccgttgg
     8761 tccgaatacc aggcaatctc ttgatagagc gtctgtgatt ccgtctacga gaggttgttc
     8821 gggtccaact accaccagat cgattttatt ctgttttaca taagatcgga tggtttcaaa
     8881 attcttgaga tcgataccca gagcgatgct ctctggtgca aacccaccgt tgcctggaaa
     8941 aacccggatg gatccgagct gcgtgctctg agaaagcttc cagaagagcg cgtgttctcg
     9001 cccgcctgag ccaatcagaa gtacgttcat actattcctc ttcttctttg cgccggacga
     9061 attccaggaa attcgcgatc actgcgtccg ggttttcgta ttgcggatag tggcctattc
     9121 ctggcaagag aaccacgtcc ggtttttgaa tcagtttctt gtattgatcc gccatgtgtt
     9181 tgccagagac tggatcttct ggcccgttga tgagtctgag cggaacacgt gagttcatga
     9241 tcgcatttac ccaacgctct ctgttctttt ctctttctgg aatgtaagaa atcaaacgat
     9301 agtagatttc atgccctttg ttcaggcgaa tgagttccca gaattgcttg agatcttctt
     9361 cagcgggctg agtgcctttc ccgaagattt cacagaacga cgcgcgaaag gcgccttcat
     9421 tgatgaacat ggaaacccat cttccaacgg ggctgagaag aagttgttgg atgagtcttg
     9481 gcctgtgtgc ttctggaaaa attccgccgt tcaagaagca aactgaaagt atttcaagtc
     9541 cgcccttgat tctttctctt ttgcgatcct gggaacgagc cagcatttcc tgggcgatgc
     9601 tcacgccgta atcgtgtgcg aagatgtgcg ctttttctat tcgcagctct cgcagtagat
     9661 tttcagcgag atctgcctgg tctagaatgg agtagttgta ttcgtgaggt ttgtctgagt
     9721 agccatagcc aatgaaatcc ggagccacgc aaagaaactg agaagtgaga gcgggccaga
     9781 ttttttccca gtcccacgaa gaagtaggga agccgtgcag aaagataact gcttctccgc
     9841 ttccgtccag gcggtaaaaa atattatgtc cattgtgacg aaaatatttc ccaccttcct
     9901 tccaggctgc ggcattcatt caccccaggc tgtggaatcg ctcgtacagg gacagaattt
     9961 tttttagatc agactccttc cggatgcctg cgatcttttt tacgccggag cagatagaaa
    10021 tgcgaagtac actgccatca caatgcacag caaagacgcc agatgattcc atcgcggact
    10081 ttccccgagg tagaagattg caaatacaat gaaaacaacc agagttacta tctcctggat
    10141 gatcttgagc tggaaggcat tgaactggcc atagcccagt ctgtttgccg gaacctgaag
    10201 gcaatattcg aagaatgcaa ttccccagct ggcgaagatc gcagcccaga tgggaatgtg
    10261 cctgaatttg aggtgaccgt accaggcgaa ggtcatgaac acattgctcg cgatgagcaa
    10321 cagtactgta gtcatactag agtatcggac ggatcg
//
LOCUS       DAOQJL010000070        10304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3382_length_10304_cov_4.156893, whole genome shotgun sequence.
ACCESSION   DAOQJL010000070 DAOQJL010000000
VERSION     DAOQJL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10304
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3382_length_10304_cov_4.156893"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..514
                     /locus_tag="PLG78_04790"
     CDS             26..514
                     /locus_tag="PLG78_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE22456.1"
                     /translation="MNKVEENRAAQYLVFQLGENSFGVDILRVKEILQFSGCTTVPRT
                     PDYIRGVVNLRGNVLPVVDLLCLFQGTPADATRKSCVIIVEIQIQGDRAEAGILVDSV
                     EQVLDMAPDMIEPAPNFGSGVPTNFISGMARFENRFVILLDLDLVLAPEEMRTFSPRS
                     NG"
     gene            507..1019
                     /locus_tag="PLG78_04795"
     CDS             507..1019
                     /locus_tag="PLG78_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNE22457.1"
                     /translation="MAEANLKITRDEPEFLLDVFLQPGDFFWGDSDTRIRTLLGSCVS
                     ITIWHPALKVGGMCHFLLPSRPANAAHAKGRYLDGAMELFAENIAKTGRNIKEFQCKA
                     FGGGNMFRERVDNVEVIGEKNIRAVEEMTQSGKLVVLSSDLGGAFYRRVQFNVWSGEV
                     WVKRKATEQA"
     gene            1016..2248
                     /locus_tag="PLG78_04800"
     CDS             1016..2248
                     /locus_tag="PLG78_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE22458.1"
                     /translation="MKSRVLIAEDDAVNGRLLESILGGSEFEATLVSSGEEALRASKE
                     NKYDLLVTDLHMPGISGIELVEKLRAEGNPVQAIILTGEEDVSTVVRAMRVGIFDYVP
                     KPVQPEEFVLRVKDAIEAGRLVQAAERATRDRDRRMERQVALSVWKEDVLGRDRDRYN
                     KTLFDNLRMSFNQGIGFGGLVTLPSLIADTSKIVDGNYLVGTDLMDLLIENARMAESA
                     MRRFEEIDQLIRGEIDLREASIAEVHTLVCRVADSLHSLLDIKSQSVVVSEPPRQSGI
                     VRISERLLTDAVRELLINAMKFSKPSSQIFVLFRRTSSHLHCSFVNTPGENQFGSRGV
                     PEEHERLIFEPFYRIVRTVDERFGTLDYGLGLSVVEKIIQGHQGRIRAGNIHDFFSKG
                     AEGESAANFELQLPLIDT"
     gene            2255..2584
                     /locus_tag="PLG78_04805"
     CDS             2255..2584
                     /locus_tag="PLG78_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE22459.1"
                     /translation="MVIDSQKKEEIVNIGGEGFFLEMLDKFIAQAIEILGQIRSAKAA
                     GNADQAKSLLHKLKGSSLTIGADDFARHVLRAYEKAQAGVMVEDEDLGLMDACLVELQ
                     AHRKTLQ"
     gene            complement(2585..3076)
                     /locus_tag="PLG78_04810"
     CDS             complement(2585..3076)
                     /locus_tag="PLG78_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22460.1"
                     /translation="MNRRTILFWLLLAPAPLCALTPDSTSILFTDAVVPGFGSFYTGR
                     PVAGSMIAGTRVGTAYLAWYFKQREREYRSAENAARIAEFYFGPGYRFKNPYGSGYYS
                     ASEFHRMAGRREFYSHLAILFHAGITVASLFATESFLSLDAEQNGPVFPEPRVRVSLS
                     VPF"
     gene            complement(3073..3483)
                     /locus_tag="PLG78_04815"
     CDS             complement(3073..3483)
                     /locus_tag="PLG78_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22461.1"
                     /translation="MKRCLFFLLLALGCDRAHTTPPGQFLLPLLRSGGARPTTPTNLR
                     ARYAASISALTMDWVGSIDPDTGLPNVVYSIYAYELPPREYYRRQDILATTTLTSYYQ
                     SADHFTGSLYFVVTAFDGGAESLPSNVAQLESSL"
     gene            complement(3480..3824)
                     /locus_tag="PLG78_04820"
     CDS             complement(3480..3824)
                     /locus_tag="PLG78_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22462.1"
                     /translation="MFLLPTCRTAYPFYVERPAGAIKVKLYLLESDYRGERNQKAVAM
                     ALSGKWAEALGTWTEMKNRKLAIDCGLFSNYAISLYMNGEPAWAAMEQAVRMCPDDNE
                     VRHNFRMLSVLQ"
     gene            3881..4123
                     /locus_tag="PLG78_04825"
     CDS             3881..4123
                     /locus_tag="PLG78_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22463.1"
                     /translation="MALLLLVACQKDPDTTPQKLATYILVCGAGVQACYTNCGNTYDA
                     NRNGTIDASEFYNFKTCTGVCDANCDVSFLYTALSK"
     gene            complement(4124..5740)
                     /locus_tag="PLG78_04830"
     CDS             complement(4124..5740)
                     /locus_tag="PLG78_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22464.1"
                     /translation="MNKAQAVSFLSIVLFAATAALAPTLGIWALPDPPRWLVLLLLAF
                     AFVPLLVDRLLPENRMILVRFPPLLIAVFSGLAAVLMWQFRQRLFLPAPNGNGDSHFL
                     IEQIPVYAELFGARLSFDEILALFVHSKFYLLCNKFGVGIIDSYAILSCGAGILYTAT
                     ALFYLNRRPLLSWLAGASVLLLTPAISIFFGYVEHYDAVSLILFVVAVMCLHLMDKDV
                     STPAWIAIGLLSALGALFHMMGGLALFPLLYFVWFKTGNRHDFIRAALKAGIPAITFL
                     AFAWIYFLLLADNRINLRESFFVKPPIYPLREFFSGKHIFDSINLILLGMPFAGGALL
                     YFLFHREMFRTTISRPDVRFLLIGLAAFFTASLFINPLLSFPSDWDLFTMFQVFGNLA
                     LAAIFAHIHSKENKLTAIALCALIICSTSTVLWIHRNHSTTPESELTVKRAIESSQGF
                     LEQIKNDPVYAKTPPQRRKTSVQARLFLYGSERKLLNKPGHKELLAEVQEESRRFDEF
                     ILLPEAEYQKAYATVWPRLTDLNRRLESIE"
     gene            complement(5733..6398)
                     /locus_tag="PLG78_04835"
     CDS             complement(5733..6398)
                     /locus_tag="PLG78_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22465.1"
                     /translation="MDRELSVERPELREKLLSIARLQYAVHPGIITPDESGKISPQRL
                     FKNPDSFHVLELGAGFGELCVEYMRSNPDHEYTAFEIKWDRIRNLLKHADADRLHPRV
                     VPVDFDWFFESMLPAHSFDRIIIYFPDPWPKRRHWKHRLIHKGFPDRLSPIMRPGADI
                     YLATDYGPYARRMLSAFRKSALKPAFPFPHYVRENPLPTRTHFEKLMSQKRKPYFMVW
                     KNE"
     gene            6456..7280
                     /locus_tag="PLG78_04840"
     CDS             6456..7280
                     /locus_tag="PLG78_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22466.1"
                     /translation="MDVKFWGVRSDVPSSLSTEEMQSRVKQAVLFAHEAWKVNPDLPP
                     DEVIASLPVHIKRLIGGETRCLEITAGTDRIIIDVGTGARRLGYDLMMRGVKGEFNVI
                     LTSTRWDHVQGWPFFVPVYIPGNSFTLFSSVSDLRERMERQQDFAFFPVGFQESPSTK
                     TFAVLTETSQHIQSFQVQCFRALDAMSSDSLRITHSGRTLLLASAALIRKRPEIAEGA
                     SLILLQDVDTSAVDVYEATRHLAARVVFTDHAPALSDREASMQPFTSASELDQMEV"
     gene            complement(7277..8413)
                     /locus_tag="PLG78_04845"
     CDS             complement(7277..8413)
                     /locus_tag="PLG78_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE22467.1"
                     /translation="MRVLSVILPTYNESRNVPILIEKLGVALAGIDYEVLVVDDNSPD
                     RTYEIVDNISKTNPRVRCIRRIHERGLSSAVVTGMSASEAQYFAVMDADLQHDESILP
                     DLLRAVREEGYDLAVGSRAAEDGSFGEWSRVRRFMSWSATLMAKILLPVQVKDPMSGF
                     FLTSRGVFEKARDQINPVGFKILLEFLGRLRGLKVKEVGYTFRTRIHGETKLSGSVIR
                     NYLVALLDLRFGHVASPVFILYCLVGASGVIVYYAVRALTDHIMISFLDTGLAPPFAR
                     IPAGAILAFIFSVTNNYAWNNYLTFYDIRHKGFRIATGVILFFLVSLVGLLVQTAVTH
                     LIHTLGMPADAASAAFARAAGISVAVITNYFLNVTFTWRARRIA"
     gene            complement(8464..9123)
                     /locus_tag="PLG78_04850"
     CDS             complement(8464..9123)
                     /locus_tag="PLG78_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22468.1"
                     /translation="MLRRLLPFLILALIFDCKSSSDEASGLLVGPAALSIGGIYSFYG
                     KSVDKTYTTSCGTAGTAFATTSSSSTSTAATTTTSSSSKKTEQTSYGIQSYYVFSNGE
                     TMSLKYDYYTTRDTFSFTPLNAATTSCNTDDLTTCNSTGRFTCTTSDNLNCGGTYAFL
                     FRNTIPALAFQGLSGSVSWKKGFSLNSSNNAVNNVVLTYNMISADGSVFKGTVECNIA
                     Q"
     gene            complement(9181..9765)
                     /gene="folE"
                     /locus_tag="PLG78_04855"
     CDS             complement(9181..9765)
                     /gene="folE"
                     /locus_tag="PLG78_04855"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNE22469.1"
                     /translation="MQAEDQGRADKLTEQYHGILQSLGENPSREGLLKTPERAAKAML
                     FLNSGYKMKLGDLVNDAVFSDVNQGMVIIRDIEVYSLCEHHLLPFFGQVHVGYIPDSK
                     IIGISKIPRIVDMFARRLQVQERLTEEILGALNQVLSPVGAGVVMQCKHMCMMMRGVE
                     KQNSVMFTSSMSGTFLTDRATRMEFLDLIRTSKN"
     gene            complement(9787..>10304)
                     /locus_tag="PLG78_04860"
     CDS             complement(9787..>10304)
                     /locus_tag="PLG78_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22470.1"
                     /translation="HLYVFFGSAGMYKMYLFVSGQNISMLKDGAARPQVYFADPEPEL
                     IKSEAKLPEREALGVIFTGSAGKYRLASVFADRVSDETLPAPTIDPIQMLASGRIFYL
                     NGETPALYHPVTGRKNLPMRARDAVILYGEDPEDQGILYQDLDGSIRFRREAFTEWEK
                     KIQGLADRLTR"
BASE COUNT         2552 a         2482 c         2808 g         2462 t
ORIGIN      
        1 cagattcgga ttttgagaga ttctaatgaa caaagtggaa gagaatcgcg ctgcgcagta
       61 cctggtcttc cagctgggtg agaactcatt tggcgtagat attttgcgcg tgaaagagat
      121 tctgcagttc tccggttgca cgactgtgcc tcgcactccg gactacattc gcggggtcgt
      181 gaatttgcgc ggcaatgtcc ttcctgttgt ggatttgctt tgcctcttcc agggcactcc
      241 tgcggacgcc acgcgaaaga gttgtgtaat cattgtggaa atccagattc aaggggatcg
      301 tgcggaagca ggaatccttg tggattctgt ggagcaggtg ctcgacatgg ccccggatat
      361 gatcgagccg gctccaaact ttggctcagg cgttcccaca aatttcattt ctgggatggc
      421 cagatttgag aatcgttttg ttattttact ggacctggat ctagttctcg ctccagaaga
      481 gatgaggact ttcagtcctc gatcgaatgg ctgaggcaaa tcttaaaatc acacgcgacg
      541 agccggagtt tctgctcgat gttttcttac agccaggaga tttcttctgg ggagacagcg
      601 atacgcgaat tcgcactctg cttggttctt gcgtatccat cacaatctgg catcctgcgc
      661 tgaaagttgg cggaatgtgc catttcctgc tgccttcgcg ccctgcgaac gcagctcacg
      721 cgaaagggag atatctggac ggtgccatgg agcttttcgc agaaaacatt gcgaagacag
      781 ggcgaaacat caaagagttt caatgcaaag cctttggcgg cggaaacatg tttcgcgaaa
      841 gagtagacaa cgtggaagtg atcggcgaaa aaaacattcg tgcggtagaa gaaatgaccc
      901 agtcggggaa actcgtagtt ctttcctcgg acctgggcgg agcgttctat cgcagagtcc
      961 agttcaatgt ctggagcgga gaggtctggg tgaaacgtaa agcaacggag caggcttgaa
     1021 atcgcgcgtt ctgatcgcgg aagatgatgc agtcaacggc aggcttctgg aatcgatcct
     1081 cggcggaagt gaatttgagg caactctagt ctcaagcgga gaagaggctc ttcgcgcgag
     1141 caaagaaaac aaatacgatt tgctggtgac agaccttcac atgcctggaa tcagcggcat
     1201 tgaactcgtg gagaaacttc gagcagaggg aaatccagtc caggccatca tcctgaccgg
     1261 cgaagaggac gtatccacgg tcgtgcgcgc gatgcgagtc ggaatctttg actatgttcc
     1321 caagcccgtg cagccggagg agtttgtact tcgcgtaaag gatgccattg aagccggaag
     1381 gctggtgcag gcggcggagc gcgccacgcg agaccgggac cgcaggatgg aacggcaggt
     1441 tgcacttagc gtctggaaag aagacgtgct cggtcgcgat cgggatcgat acaacaagac
     1501 tctgtttgat aaccttcgca tgagtttcaa tcagggaatt ggatttggcg gtcttgtaac
     1561 actcccttct ctgattgcgg acacttcaaa gatcgtagac ggcaattatc tcgtgggtac
     1621 agatctcatg gatctgttga tcgaaaacgc gcgcatggca gaatctgcaa tgcggcgatt
     1681 tgaagagatc gatcagctga tccgaggaga aatcgatcta agggaagctt cgattgcaga
     1741 agtgcataca ctcgtttgca gagtggcaga ctcattgcac agccttctgg atatcaagag
     1801 tcagagcgtt gtggtgagcg agccgccgcg ccagagtggg atcgtgcgaa tttcagaacg
     1861 acttctcacg gatgcggtcc gggaactctt gatcaatgcc atgaagtttt caaagccttc
     1921 ctcccaaatc tttgtactat ttcgcagaac atcgagccat ctgcactgct ctttcgtaaa
     1981 cactccaggt gagaatcaat ttggatccag aggtgtgccc gaggaacatg agagattgat
     2041 cttcgaacct ttctatagaa tcgtccgcac tgtggacgag cgattcggga cgctggacta
     2101 tggtcttggt ctttctgtcg tggaaaaaat cattcaggga catcagggaa gaattcgagc
     2161 gggcaacatt cacgatttct tcagcaaggg tgcggagggt gagtcagcgg ccaattttga
     2221 gttgcagctc ccgctgatcg acacatagtc gctcgtggtc attgattccc agaaaaagga
     2281 agaaattgta aacatcggag gggaaggatt cttcctcgag atgctggaca aattcatagc
     2341 gcaggcaatt gaaatcctgg gtcaaattcg gtctgcgaaa gcggccggaa atgcggacca
     2401 ggcaaaaagt ctgctccaca aattaaaggg atcgagtctg accattggcg cggatgattt
     2461 tgcacgccac gttcttcgcg cgtatgaaaa agctcaggcc ggagtgatgg tcgaagatga
     2521 agatctcggc cttatggacg cttgcctggt agaactccag gcacaccgca agacactcca
     2581 atagttaaaa aggaacggac agagatactc gaactcgcgg ttcgggaaac accggaccgt
     2641 tttgctccgc atcgagggaa agaaagctct ctgtcgcaaa cagactggcc acggtaatcc
     2701 cagcatgaaa cagaatcgcc aggtgagagt agaattctct tctgcctgcc atcctgtgaa
     2761 actccgaggc actgtagtag ccggaaccat acggattctt gaatcgatag cccgggccaa
     2821 agtagaactc cgcgatccgc gctgcgtttt ctgcggatct gtactccctt tctctttgct
     2881 tgaagtacca tgccagatag gctgtgccga ctctcgtgcc ggcgatcatg gagccagcca
     2941 ccggtctgcc tgtgtagaaa gatccgaagc ctggcacaac tgcgtctgta aacagaatgg
     3001 atgtggaatc cggtgtaagg gcgcacaggg gtgctggggc cagcagcagc cagaatagga
     3061 tggtgcgcct gttcaaagag atgactccag ttgtgccacg ttagacggca atgactccgc
     3121 acctccgtca aatgctgtga ctacaaaata cagtgatcct gtaaaatgat ccgctgattg
     3181 atagtaagat gtgagcgtag tggtcgcaag aatgtcctgg cgtctgtagt attcgcgcgg
     3241 aggaagttca tacgcgtaaa tggaatagac tacgtttgga agccctgtgt caggatctat
     3301 gcttccaacc caatccatcg tcaaagcgga aatggacgca gcgtatcgcg cacgtaggtt
     3361 cgtgggagtg gtgggccttg ctcctccact gcgcaacaga ggaagaagaa attgtccagg
     3421 cggcgtagtg tgtgcacgat cgcagcccag agccaggaga aggaagaaca aacaccgttt
     3481 cattgaagta cgctcagcat acgaaaattg tgtcttactt cattgtcatc cggacacata
     3541 cgaactgcct gttccatggc ggcccaggct ggttcgccgt tcatataaag ggaaatagcg
     3601 tagttggaga agaggccaca gtcaatggct agcttcctgt ttttcatttc agtccacgtg
     3661 cccagcgctt ctgcccattt cccggaaagt gccattgcaa ctgccttctg gtttctttct
     3721 cccctgtagt ctgattcgag caggtagagc ttcactttga tcgcgccggc cggccgttcc
     3781 acgtagaaag ggtaggcagt tctgcaggta ggaagaagaa acaaaaggac tatgcagttt
     3841 ctgagccgag ccgaaaactg gttgtatgcg tgtcgtattt ttggcattgc tcttgctggt
     3901 tgcctgccag aaggatcctg acacaactcc tcagaagtta gcaacctata ttcttgtttg
     3961 cggcgcgggc gttcaggcat gctataccaa ctgcggcaat acttacgacg cgaatcgaaa
     4021 cggaaccatc gacgcatctg agttttacaa cttcaagacc tgcaccggcg tgtgcgacgc
     4081 caactgcgac gtgagcttcc tctacaccgc tctctcaaag taatcattct atcgattcca
     4141 gtctgcgatt cagatccgtc agtctgggcc agactgtggc gtacgccttc tggtattctg
     4201 cttccggaag caatatgaac tcatcaaagc gtctggattc ttcctggact tccgcgagaa
     4261 gctctttgtg gccgggcttg ttcagcagtt ttctttcaga tccgtaaaga aagagtctcg
     4321 cttgaacaga tgtctttctt ctctgcggag gtgtcttcgc atagaccgga tcattcttga
     4381 tttgctcaag gaacccctgg gaactttcaa tggctctttt tacggtcaac tcgctttccg
     4441 gagttgttga atgattgcga tgaatccaga ggacggtgct tgtgctgcaa atgatcagtg
     4501 cgcaaagagc gatcgccgtt agtttatttt ctttagaatg aatgtgcgca aaaatcgcgg
     4561 ccagagcgag attcccaaag acctgaaaca tggtgaaaag atcccagtca gatggaaagc
     4621 tgagcagagg gttgatgaac agcgacgcgg tgaagaatgc ggccagacca atgagtaaga
     4681 atcgaacgtc tggtcttgag atcgtagtgc gaaacatttc tctgtgaaac aagaaataca
     4741 gaagggcacc tccggcaaat ggcataccaa gcaagatcaa gttgatcgag tcgaagatgt
     4801 gcttgccgga aaagaattca cgtaatggat agatgggcgg ctttacgaaa aacgattcgc
     4861 gcagattgat gcgattgtcg gccaggagta gaaagtagat ccaggcaaag gccagaaagg
     4921 tgatggcggg aattcctgct ttcagggccg cgcgaatgaa atcgtgcctg tttccagtct
     4981 tgaaccatac aaaatagaga agcggaaaaa gtgcaagccc tcccatcatg tgaaagagcg
     5041 caccaagcgc ggacagaagg ccaatcgcaa tccaggcagg agtagaaaca tctttgtcca
     5101 tgagatgcaa acacatcacc gctaccacga atagaatcaa ggacacagcg tcgtagtgtt
     5161 ccacataacc aaagaagatg gaaatggcag gtgtaagtaa cagaacagat gcaccggcca
     5221 accacgaaag caaaggtcgt ctgtttagat aaaagagagc agtggcagtg tatagaattc
     5281 ctgcgccgca gctcaagatc gcatagctat caatgatgcc cacgccgaat ttgttgcaga
     5341 gaagatagaa tttgctgtgc acgaacaaag caaggatttc atcgaagctc agtcttgcgc
     5401 cgaataactc tgcgtagacg gggatttgtt caatgaggaa gtgactgtct ccatttccgt
     5461 ttggagcagg caaaaacagt ctctgtctga attgccacat caagaccgca gccagtccgg
     5521 aaaaaacagc aatgagcaga ggagggaatc gaaccagaat cattcgattt tctggaagca
     5581 gtctatctac aagcaaaggg acaaaagcaa aggcaagaag caaaaggacc agccagcgcg
     5641 gagggtccgg cagtgcccag attcctagag tcggggccag ggcagctgtg gctgcgaaga
     5701 gcacaatgga aaggaaggaa accgcttgag ccttattcat ttttccatac catgaagtag
     5761 ggtttgcgtt tttgcgacat cagcttttcg aaatgcgtgc gagtgggcag ggggttctcc
     5821 cttacgtaat gaggaaacgg aaaagcaggc ttgagagcgg acttgcgaaa ggcactcagc
     5881 atccgcctgg catatggacc gtaatccgtc gccagataga tgtccgctcc aggccgcatg
     5941 atcggactga gtctatccgg gaagcctttg tggatgagtc tgtgtttcca gtgtctcctt
     6001 ttgggccagg gatctggaaa atagatgatg attcgatcga atgaatgcgc aggaagcatc
     6061 gattcaaaaa accagtcgaa gtcaacggga accacacgcg ggtgaagccg gtcagcatct
     6121 gcgtgcttga gtagattgcg aatccgatcc cacttgattt caaacgcagt gtactcatga
     6181 tctggattcg atcgcatgta ttcgacgcaa agctctccaa atcccgcgcc cagttctaga
     6241 acgtggaagc tatccggatt cttgaacagt ctttgcggac tgatctttcc ggattcatct
     6301 ggcgtgatga taccagggtg aacagcatat tgcaatcggg cgatcgatag aagtttttcc
     6361 ctcagctctg gcctctctac tgatagctct ctatccatcc ggagtccaga aaatcttttg
     6421 gtttacaggg agccatcacg ttcctggata ccagcatgga cgtgaaattc tggggtgtga
     6481 ggtcggatgt tccttcctcg ctttcgacag aggaaatgca gtccagagtc aagcaggctg
     6541 ttctgttcgc gcacgaagcc tggaaggtaa accccgatct tccgccggat gaagtcattg
     6601 cgtctcttcc ggttcatatc aaacgtctca taggtggaga aacgcgctgc ctcgaaatca
     6661 cggcgggaac agaccgcatc atcatagatg taggcacagg cgctcgcagg cttggctatg
     6721 atttgatgat gcgcggagtg aagggcgaat tcaatgtaat cctgacatcc actcgttggg
     6781 atcacgtgca gggttggcct ttctttgttc ccgtttatat tccaggaaat tcattcactc
     6841 ttttttcatc tgtgagcgac ctgcgcgaaa gaatggaacg ccagcaagat tttgcgttct
     6901 tccccgtggg gtttcaagaa agtccttcta ctaagacgtt tgcagttctc acggaaactt
     6961 cgcagcacat tcagtcgttt caggtgcaat gctttcgcgc gctcgatgcc atgtcctcgg
     7021 attctctgag aatcactcat tctggcagga ctctcttact cgccagtgcg gcactcattc
     7081 gaaagcgccc agaaatagcg gaaggggctt ctttgattct actgcaagat gtggatactt
     7141 ctgccgtgga tgtctacgaa gcaaccaggc atctggcggc gcgagttgtg tttacagatc
     7201 atgctcctgc tctgtctgat agagaggcgt ctatgcagcc atttacatct gcgagtgaac
     7261 tggaccagat ggaagtctag gcgattcgcc gcgcacgcca ggtgaaagtc acgttcaaga
     7321 agtaatttgt aatcaccgct acggaaatcc cggccgctcg cgcaaaggct gcacttgccg
     7381 catctgcagg catgccgaga gtgtggatga gatgagttac tgcggtctgg accagtaaac
     7441 caaccagact caccaggaag aagaggatca ctcctgttgc gatgcgaaac cctttgtgtc
     7501 tgatatcgta gaaagtcaga tagttattcc acgcatagtt gtttgtgacg ctgaatatga
     7561 aagcgagaat tgcaccagcc ggaatgcgcg cgaagggcgg tgccagaccg gtgtccagaa
     7621 aagagatcat gatgtgatct gtcagtgcac gcacagcata gtaaacaatc acgccggaag
     7681 cacccacgag gcaatagaga atgaaaacag gactggccac atgaccaaat ctgagatcca
     7741 gaagagccac gaggtaattg cgtatgacgg atccgctcag ttttgtttct ccgtggatgc
     7801 gtgtgcgaaa cgtgtaacct acttctttta ctttcagacc gcggagtctg cccaggaact
     7861 cgagcaggat tttgaatccg actggattga tctggtcgcg cgccttttcg aatacacccc
     7921 tggacgtgag gaagaagcca ctcattgggt ctttgacctg aacaggcaga agtatctttg
     7981 ccatgagagt cgcagaccag ctcatgaatc ttcgcacacg ggaccattcg ccaaagcttc
     8041 cgtcctctgc tgcgcgcgag ccgactgcca gatcgtatcc ttcttctctg actgcgcgaa
     8101 gcaagtcagg aaggatggac tcatcgtgct ggaggtccgc atccatcaca gcaaagtatt
     8161 gagcttctga tgcgctcatt ccggtgacaa ccgcggaaga aagtcctctc tcgtggatcc
     8221 gccgaatgca gcgaacacgc ggattggttt tggaaatgtt gtctacgatt tcgtacgtgc
     8281 gatcggggct gttgtcatct acgactagaa cttcgtagtc tatgccagcc agtgcaacgc
     8341 ctaacttctc aatgaggata gggacgttcc tggattcatt gtatgtgggt agaatgacgg
     8401 agaggactct cacgcgccca ggatattcca aggctcgttc agggcgacaa aattaaacaa
     8461 gaatcactgg gcgatgttgc attccactgt gcccttgaag acgctgccgt ctgcgctgat
     8521 catattgtag gtgagcacta cgttgttgac tgcattgttc gaggaattga gagagaaccc
     8581 ttttttccag gagacagaac cgctcagacc ctggaaggcg agggcgggga tcgtattgcg
     8641 aaatagaaag gcgtaggttc caccgcagtt gaggttgtct gaggtcgtgc aggtgaatct
     8701 gcccgtgcta ttgcacgtgg tcaaatcgtc cgtattgcag gaagtggtgg ccgcgttgag
     8761 tggggtgaag ctaaacgtat cgcgcgtagt gtagtaatca tacttcaggc tcatggtttc
     8821 tccattggag aacacgtagt aactctgaat tccgtagctg gtctgctctg tctttttcga
     8881 gctgctgctc gtggtggtcg tggctgccgt agaagtgctg cttgagctcg tggtcgcaaa
     8941 cgcagttcct gccgttccgc agctagtggt gtaagtctta tctacgctct ttccgtagaa
     9001 actgtagatg cccccgatgg agagggcggc cggtccaacc agcagacccg aagcctcatc
     9061 gctggaggac ttgcagtcaa aaatcaaagc aagaatcagg aatggcagca gtctacggag
     9121 catattctat tgtcggagtc tgatcctcca atctccatgg agctctgcca gcagttttct
     9181 tcagtttttt gaggttctta tgagatccag gaactccatt ctggtagccc gatccgttag
     9241 aaatgtgccg ctcatgctac ttgtgaacat gaccgagttt tgcttttcta ctccgcgcat
     9301 catcatgcac atatgcttgc actgcatgac gactccggcc cccactggag agagaacctg
     9361 gttgagagca ccgaggattt cctccgtcag gcgttcctgg acctggagcc tgcgtgcgaa
     9421 catgtcgacg attcgcggga tttttgaaat tccaatgatc ttactatccg ggatataccc
     9481 tacgtgcacc tggccgaaga agggcagaag gtggtgttcg cacaggctgt acacttctat
     9541 gtcgcgaatg attaccattc cctggtttac gtccgagaag actgcgtcgt tcaccagatc
     9601 gcccagcttc atcttgtagc cagaattcaa aaagagcatg gccttggccg cgcgttccgg
     9661 agtcttgagc aatccttcgc gcgaagggtt ttctcccagg gactggagga tgccgtggta
     9721 ttgctctgta agtttgtctg ctcttccctg gtcttctgcc tggatggaat ctatgtccac
     9781 tgtttcctac ctggtgagcc tgtctgcgag gccctgtatt ttcttttccc actccgtgaa
     9841 cgcctcgcgg cgaaagcgaa tcgaaccatc cagatcctgg tagagaatcc cctggtcttc
     9901 tggatcctct ccgtacaaga tgaccgcatc gcgcgctcgc atgggcagat tcttccttcc
     9961 tgtgacaggg tggtagagag cgggagtctc accatttaag taaaagattc ttccggaggc
    10021 gagcatttga atgggatcaa tggtgggtgc cggcagggtt tcatctgaaa cgcgatctgc
    10081 gaagacggaa gccaggcgat acttgcctgc actgcctgtg aagatcactc caagcgcttc
    10141 gcgttctgga agtttcgcct ctgatttgat gagttctggt tcagggtctg cgaagtatac
    10201 ctggggtctg gccgctccgt cctttagcat ggatatattc tggcccgaaa cgaataaata
    10261 catcttgtac attccagcgg atccaaagaa tacatacaga tgat
//
LOCUS       DAOQJL010000071        10248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3404_length_10248_cov_4.544491, whole genome shotgun sequence.
ACCESSION   DAOQJL010000071 DAOQJL010000000
VERSION     DAOQJL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3404_length_10248_cov_4.544491"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLG78_04865"
     CDS             complement(<1..409)
                     /locus_tag="PLG78_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNE22471.1"
                     /translation="MSAIFIDHYAALEIPRTAAGEDVRRAFRRLARVHHPDRGGDALR
                     FHEIYTAYRLLSNEDDRARFDRQLVLRHVRSIMGQRKFIHPSRIVVPTTMAALAKRGL
                     LRKQFRGRDRRMHLRVDYDLELPLGDDELKLPLL"
     gene            527..832
                     /locus_tag="PLG78_04870"
     CDS             527..832
                     /locus_tag="PLG78_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22472.1"
                     /translation="MRLAILLVLIFTCGLLAQKDIRSALSADQAAARIEQLLASKPKI
                     VKKYEKKDDTRFLTLTQTTAASFAGSRLPPGQFTLLYMFTRDAEKTIARFEPGKNGV"
     gene            844..1593
                     /locus_tag="PLG78_04875"
     CDS             844..1593
                     /locus_tag="PLG78_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22473.1"
                     /translation="MTHPDLFTDPNTNWSLVEQVACKDARDRRHFVHGFALMRGMGRL
                     RPGLERVDEAVSNSDLRPWKNDRQKLEFMFKLAGEIAGNAPVSDACMERIEASIHGKT
                     AQRSADFMEGDPCTDALRSCGNCHLPAKNNCNFTVTYWNEDRQKEANGTVVKEDLRTI
                     TLGGETQQRTIAREDVIQTRWENGADPVEVWWNFKSISSRGSIAKKMLALSAEKQTGC
                     ATCHIGHGDFRMTPAGEEFAKTGVLRSPAQK"
     gene            complement(1795..2586)
                     /locus_tag="PLG78_04880"
     CDS             complement(1795..2586)
                     /locus_tag="PLG78_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22474.1"
                     /translation="MKIYRVFFIILTLAPLSLFAIDGLTRVPEPTPEEQAAYKDVINH
                     IADIQKDIAAYYTDLSEKGRILRTRDASGKEVGGPYSEFEITSEVQTYYGQRDRYVYT
                     ERIHIDWNLQPGTQAGSVVASVKSVVLTQRRGRVGHGYVLKRRIAADVLPKVESESDA
                     LPVNLIVNELLDSGKGLLVNFRFPEAKDVRDKTGENAEVIEVDGQKKKIDLIYVRSHA
                     EKMRMLREYRDALKLTWRRVDWEVRSEKARNARELERILRRQTDS"
     gene            2719..3054
                     /locus_tag="PLG78_04885"
     CDS             2719..3054
                     /locus_tag="PLG78_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22475.1"
                     /translation="MQIKLKSNNQVTLFELEGSLDIYTSLDLKASLEENVKAKGTTVI
                     LDMEKLTYIDSSGIGIVIKALNYVQGLSGKLIVANLKPPIEKVFRVSGLISYFEILNG
                     EELKKRYPM"
     gene            complement(3055..3645)
                     /locus_tag="PLG78_04890"
     CDS             complement(3055..3645)
                     /locus_tag="PLG78_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22476.1"
                     /translation="MKSLLFFIMILGSPLLAGSPREINFGSEARYGRFMSFIELESWA
                     TGRNLTVLRDCPFRDLDDGEAEIIPAEGMDFSLSSPRDGRVYLYLDMVVFRPAASYNP
                     LTDAIHCQTGGEGVRMNNDRTQELKQVRWLSVEVNGRTLKTVYVGGDVYLRSPIVIPI
                     EREVARGGTLNVRLTVSPGDGFFAIWDTFVSVSPDG"
     gene            3714..4634
                     /locus_tag="PLG78_04895"
     CDS             3714..4634
                     /locus_tag="PLG78_04895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNE22477.1"
                     /translation="MHIIHDLSTFEPPWTSCALTPGVFDGLHRGHRALIQRLLKKKVD
                     ARILVTYHPHPDLVLGKRKDPEGYELFTYEEKLSLFQSYPLEAVVFLPFTVELARMTA
                     LRYLKEILLGKLRAKNIVIGYDQCFGRGRKGDYKFLKQMSRRYDFEVDRVKAVRGFGP
                     VISSSQIRTYLKQGLIEKAANLLGHDYFLTGTVVRGFQRGRLIGFPTANLEVPRSKVI
                     PAEGVYTAKAEWGGRYYRAMVNIGTNPTFEGKSVSVEAHLLDFDGDLYGQQLRIHFRK
                     HIRNEIKFSSVDDLKSRLEKDRIIARKLKV"
     gene            4647..5231
                     /locus_tag="PLG78_04900"
     CDS             4647..5231
                     /locus_tag="PLG78_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HNE22478.1"
                     /translation="MHNLQEVFERINLINSEDERRLVDPETGTSLPQEVLYSKRMTHW
                     LNVLAPNASDELRVAVFAQHIARFKHPRKTYPEGRTGYLKWKAALLQIHAETTAAILE
                     EMKFPNEFIARVSRLLKKQGLGRDPETQILEDCACLVFIEMFYMDFAPTQGREKMIDI
                     VQKTLRRMSESAKEHAMKLPAAVHVAKLLESSSA"
     gene            5228..5830
                     /locus_tag="PLG78_04905"
     CDS             5228..5830
                     /locus_tag="PLG78_04905"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNE22479.1"
                     /translation="MNSRESPGGSSAHASHVSGVVLCGGHSSRMGRDKGLIERNGIPW
                     ARIIYDSLSLILKNVLVSIREEQRATYEKVFSSEVLIGDSVDTVQGPMRGLISSFLKC
                     DTDILLVGCDMPDLRQSLSHLLQLREENPDYDCLAYRTARVEPLCAIYGKNILKQLRD
                     HHPETFSLERLLERSRTLFIQADENQTAELRNYNEPGPAL"
     gene            complement(5803..6270)
                     /locus_tag="PLG78_04910"
     CDS             complement(5803..6270)
                     /locus_tag="PLG78_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNE22480.1"
                     /translation="MMTGSQATQSIHPRLLTDEPIDLGLFTKQSDLRAGAQVIFAGRV
                     RNHHGGREVNHLEYSAHQKLAPIMISQILREESDKLRLYEAMAVHRIGRVGLGEIAVV
                     VVTSAAHRAEAYEANQRIIHRIKHEVPIWKKEYFADGSWEWGTCDHSAGPGSL"
     gene            complement(6270..6506)
                     /locus_tag="PLG78_04915"
     CDS             complement(6270..6506)
                     /locus_tag="PLG78_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22481.1"
                     /translation="MEIRIQFFASLRDTFGEWLDLSVKEGASAADVLTALADLNPQAA
                     SLLGVSCCATDDRVLGKGEQVPATICVFPPMSGG"
     gene            complement(6509..7495)
                     /locus_tag="PLG78_04920"
     CDS             complement(6509..7495)
                     /locus_tag="PLG78_04920"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNE22482.1"
                     /translation="MTKQSLAESLIVSGAGPLIADAMGRSFATLRLSVTDRCNFACVY
                     CAGDSPFKADSRPFRSAEEIFSSAMPLIEIAGIKKIRITGGEPTLHPGLVDLVALFGK
                     AGLNSIHLTTNGWNLRELVLPLRDAGLTSLNVSLDAAGEEGFRRMARRGGFAGVLHGI
                     DVARALLPVKINCTLVRGQNENEILPVFKLARSLGVTVRYLELMAMGPLRKDARSLLV
                     KEQTILDAIATEESFTPVPTEKSSTAHYFRLTDGYMFGVIANESMPFCSDCNRLRMDA
                     RGNIYGCISDARGININGLQKSELENALRTALSRKQPVRFVGSEKSMQELGG"
     gene            complement(7498..8679)
                     /locus_tag="PLG78_04925"
     CDS             complement(7498..8679)
                     /locus_tag="PLG78_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNE22483.1"
                     /translation="MMIDSASALQLLLDRTPTTVRTESVLLEHSARRVLAQDILADRH
                     YPPFHRSAMDGFAVRFDDLIASRSLSIFATILAGDDPPTSVPPGSAVRIMTGAAVPED
                     LDTVVKVEESQVTGDHVVLSPAKKIWQNIAKRGEDAEPGDQIVLAGRIIDASVQAAAA
                     SCGQDSMRVLAPRIHLITTGNEIVEPGAVPRAVEIRNSNRYTIASLFSYYALHPATVS
                     HVADDPAELKKRLESSLDSELLVLSGGVSAGEADYLPGVMEKCGVEKLFHGVSIRPGK
                     PVWAGFFDSCMVIALPGNPVSTHVTARLFVEPVLRKILGLRREQYPFKRLAKSIAKNH
                     KLQEYLRVTLREDDTLESVPHNGSGDAFASVRATGLAVMPPQSSSIPVGSPVEYIPFL
                     W"
     gene            complement(8669..9580)
                     /gene="moaCB"
                     /locus_tag="PLG78_04930"
     CDS             complement(8669..9580)
                     /gene="moaCB"
                     /locus_tag="PLG78_04930"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HNE22484.1"
                     /translation="MKDIAARINTLRIATASARLKYSPDVEEIVARGITPKGNIYEIS
                     KTAALIGAKQTPMLIPFCHPLSIDGMSVTFEKEPGSIIVRVEAKSIGRTGLEMEVLTA
                     ASVAALNLYDLLKPYDAEMSIEKIQLDKKTGGKSEKWNRIKEGTTAAVLTVSDTVAAG
                     KKPDGSGTAIKDRLTSLGVTVADYRVVADEPQQIQQWVEKWIAEKIPFIFTTGGTGLG
                     PRDVTVETLKKLIQRPVPGIAEAMRVHGGQRTPVAMLSRSVAGTADQSIIVAFPGSTR
                     GVTESMDAIFPAVFHGAGILRGGRHDD"
     gene            complement(9628..10020)
                     /gene="nirD"
                     /locus_tag="PLG78_04935"
     CDS             complement(9628..10020)
                     /gene="nirD"
                     /locus_tag="PLG78_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNE22485.1"
                     /translation="MNNANLNWMRAGAEADFPRDGGVVVKLGNRQIAVYRFENETDTW
                     YACDNVCPHQNEAVLGRGIIGDKKGEPVVACPMHKKVFSLKSGNCISDAGYSVNVYPV
                     QLKDGSVFVMVHETASNAESLPAEQKVR"
     gene            complement(10024..>10248)
                     /locus_tag="PLG78_04940"
     CDS             complement(10024..>10248)
                     /locus_tag="PLG78_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22486.1"
                     /translation="SVIIDDSLGICAELEQQMELLVKNYKCEWNEVVSSPALRARFRP
                     FVNTDETDETIRFEYERGQKKPAEPVGAGV"
BASE COUNT         2616 a         2769 c         2488 g         2375 t
ORIGIN      
        1 cgagtaaagg tagtttcagt tcatcgtcgc ctaacggcaa ttccagatca tagtcaacgc
       61 gaaggtgcat acgccgatcg cggccgcgaa actgttttcg caacaatcct cgctttgcga
      121 gcgcggccat ggtggtagga actacgattc gcgacggatg aatgaatttt ctctgaccca
      181 tgatggatcg aacgtgacgc aagacaagtt ggcgatcaaa gcgcgctcga tcgtcttcat
      241 ttgatagaag ccgataagca gtgtagattt catgaaaacg cagtgcatcc ccgccacgat
      301 ccggatgatg cactctggca agtctgcgaa acgcacgacg cacatcctct cccgcggcag
      361 tcctgggaat ctcaagcgca gcatagtgat caatgaaaat ggccgacact tgcttcagac
      421 ttcttcacaa gggggacgat gcaacagttt tttaagaggc ttgcccgcct tgccggagag
      481 ggctgccccc ggctcaagtt ttcgctgaaa gcatcgaaat tgaagcatgc gccttgccat
      541 tctgctcgtt ttgattttca cttgcggcct tcttgcacag aaggatatac gatccgctct
      601 gagcgcagat caggcagcgg ccagaatcga acagttgctt gcatccaaac caaagatcgt
      661 caagaagtat gaaaagaagg acgacactcg attcttaaca ctcacacaga cgacggcggc
      721 gagtttcgcg gggagtcggt tgcctccggg gcagttcacg ctgttgtata tgtttacgcg
      781 cgatgcggag aagacgatcg cgcggttcga gccaggcaaa aacggagtct gagcactctc
      841 cagttgactc atccggattt attcacagac ccgaacacaa actggtccct ggtagaacag
      901 gtagcctgca aagacgcgcg agatcgtcgc cacttcgtgc atggctttgc cctgatgcgc
      961 ggcatgggca gattgcggcc gggattggag cgcgtggatg aggcagtttc aaactcggat
     1021 ctcagaccat ggaagaatga tcgccagaaa ttagagttca tgttcaagtt agccggagaa
     1081 attgcgggga atgcgccggt ctctgacgca tgcatggaac gaattgaagc gtccattcac
     1141 ggaaagacag cgcaacgctc ggcagacttt atggaagggg atccgtgcac agacgcactg
     1201 agatcgtgcg gaaactgcca tcttccggca aagaacaact gcaattttac agtcacctac
     1261 tggaatgagg acaggcaaaa agaagcaaat ggaaccgtag tcaaagagga tctgcgtact
     1321 ataacgttag gcggcgaaac acaacaacga accattgcac gcgaagatgt aattcagacc
     1381 cgctgggaaa atggcgcaga tccagtcgag gtctggtgga acttcaaatc tatttcttca
     1441 cgcggatcta tagcaaagaa gatgcttgca ctttcagcag aaaaacaaac gggctgtgcg
     1501 acctgccaca ttgggcatgg cgactttcgc atgactcctg caggcgaaga atttgcaaaa
     1561 accggcgtgc tacgttcccc cgcccagaag tgaggatccg tccgaaccaa atgcgaaaac
     1621 gggcgcaggt ctcctctatt ttatcatacg ctaaaagtga gacagtgctc tgttgacttc
     1681 tgtgaactgg atggataggt ggcgccgggt tgccgggccg gagttcaccc accgccataa
     1741 ccacccacat ccgggggtgg gcggagtctg cccccgcgcg ggaggcagga gagatcagga
     1801 gtctgtttgt cgacgcagga tgcgctcgag ttcacgcgca ttgcgcgcct tttctgagcg
     1861 aacttcccag tccacacggc gccaggtgag ctttagagcg tcgcgatatt cgcgcagcat
     1921 ccgcatcttt tcagcgtgcg aacgcacata aatcagatcg atcttctttt tctggccatc
     1981 cacttcgatg acttctgcat tttcgccggt cttatcgcga acatccttgg cttctggaaa
     2041 gcggaagttg acaaggaggc ctttgccaga atcgagcagt tcatttacaa tcaggttcac
     2101 tggcaatgcg tcgctctctg attccacttt tggcaataca tctgcggcaa tccggcgttt
     2161 caaaacatag ccatgaccga ccctaccccg gcgctgcgtc agtacgactg actttacgct
     2221 ggcgactaca cttcccgcct gggttccggg ctgcaggttc caatcaatat gaatgcgctc
     2281 cgtgtaaacg taccggtcgc gctggccgta gtaagtctgc acttctgaag taatctcgaa
     2341 ctcggaatac ggcccaccga cctctttacc agatgcgtcg cgggtgcgaa gaatccggcc
     2401 tttttcgctt agatccgtgt aataagcagc tatgtccttc tgaatgtcgg cgatatggtt
     2461 gatgacatcc ttgtaagcgg cctgttcttc aggcgtgggc tccggaactc tcgtcaagcc
     2521 gtcgatggcg aacagagaaa gtggagccag agtcagaatg ataaaaaata cgcgataaat
     2581 cttcatagcc gtccccggtt actcttactt tcggcctggg caccgcaatt cttacgtccg
     2641 acccggcaat tctcatcgcc gcagatactt tttttcattg attaccccag accaacatgg
     2701 aatatcgccc ctatgccgat gcagatcaag ctgaaaagca acaaccaggt aacactgttt
     2761 gagctcgaag gaagcctcga catctacact tctctggatt tgaaagcttc tctggaagaa
     2821 aacgtaaaag ccaagggaac cacagtgatc ctggacatgg aaaagctcac ctacatcgac
     2881 tccagtggaa tcggcattgt gatcaaagcg ctgaattatg tccagggatt gagcggaaag
     2941 ctcattgtag ccaacctaaa gccacccatc gaaaaagtgt tccgcgtaag cggtctgatc
     3001 tcttactttg agatcctcaa tggcgaagag ctgaaaaagc gctaccctat gtgatcagcc
     3061 atccgggctg acacttacaa acgtatccca gatcgcaaag aaaccgtctc cgggggacac
     3121 cgtcaggcga acattcaaag tgcctccgcg tgccacctct cgctcaatag ggatcacaat
     3181 gggagaacgc agatatacat cgccacccac gtacactgtt ttcaaggttc ttccattcac
     3241 ttccactgag agccagcgca cttgcttgag ttcctgagtg cgatcgttgt tcattcgcac
     3301 gccttcgccg ccggtctggc agtgaatcgc gtctgtgaga ggattgtagg acgcagcggg
     3361 gcgaaacacc accatgtcta gataaagata cactctcccg tcgcgcggag acgaaagaga
     3421 gaagtccatt ccttctgcgg gaatgatctc agcttcacca tcgtccagat ctctgaacgg
     3481 acagtcgcga agcacagtga ggtttcgccc ggtcgcccag ctctccagct caatgaagga
     3541 catgaatcta ccgtatcgag cctcgctgcc aaaattgatt tcacgaggag agccagccaa
     3601 caatggagag cccagaatca tgataaagaa tagaagagac ttcatgctac ctcggaattc
     3661 agcttggcga aactggccca caatacacgt ttcggcaggt ctttcaactc tctatgcaca
     3721 taatccacga tctgagcact tttgagccgc catggacatc gtgtgctctg acacctggag
     3781 tatttgacgg cctccacagg ggtcatagag ctctcattca acgcctctta aaaaagaagg
     3841 tagatgcgcg catcctggtc acataccatc cacacccaga cctggtgctc ggaaaacgca
     3901 aagacccgga aggatacgag ctgtttacct acgaagagaa attgtctcta tttcaaagtt
     3961 atcctctcga agcggtcgtt tttttgcctt tcacagtaga actcgccagg atgacggcgc
     4021 tgcgatatct caaagagatt ctcctgggca agctccgggc aaaaaacatt gtaatcgggt
     4081 atgatcaatg ttttgggcgc ggaagaaagg gagattataa atttcttaaa caaatgtcca
     4141 ggcgatacga cttcgaagtc gaccgtgtca aagcggttcg gggttttgga ccggtcatct
     4201 ccagttcgca aattcggaca tatctcaagc aaggcctcat tgaaaaagcc gccaacctgc
     4261 tagggcatga ctactttctc acaggcactg tggttcgggg ttttcagcgt gggcgtttga
     4321 ttggatttcc aactgcaaac ctggaagtgc ctagaagcaa agtgatcccc gcagaaggag
     4381 tctacacggc gaaggctgaa tggggtggca gatactacag ggccatggtg aacattggaa
     4441 ccaacccaac gtttgaagga aaatccgtct ctgtagaagc gcatttgctc gactttgatg
     4501 gagacctcta cgggcagcaa cttcgcattc atttccgcaa acacattcga aacgaaatca
     4561 aattctcatc tgtagacgat ctgaaatcac gtctcgaaaa agataggatc atcgcgcgaa
     4621 aactaaaagt ttaatcggga gcattcatgc acaatctgca agaggtattc gagcgaatca
     4681 atctgatcaa ctccgaggat gaaagacgac tcgtagatcc agaaacaggc acatctctgc
     4741 cacaggaagt actctattca aaaaggatga cgcactggtt aaacgttctc gctccaaatg
     4801 catccgacga acttcgcgtc gcagtcttcg cgcagcatat cgccagattc aaacatcctc
     4861 gcaaaacata tccggaagga cgcacaggct atctgaagtg gaaggcggct ctgctgcaaa
     4921 ttcacgcgga aaccacagcc gccattctcg aagaaatgaa atttccgaat gaattcattg
     4981 cgcgagtttc caggctgctg aagaaacagg gcctcgggag agatccggaa acgcaaattc
     5041 tagaagattg cgcatgcctc gtattcattg aaatgtttta catggacttt gcgcccacgc
     5101 agggtcgcga aaagatgata gacattgttc aaaagactct tcgcagaatg tctgaatccg
     5161 cgaaagaaca tgcgatgaaa cttccggccg cagttcatgt tgcaaagcta cttgaatctt
     5221 ccagcgcatg aactcgcgag aatcccctgg aggttcgtca gctcacgctt ctcacgtttc
     5281 aggcgtggtg ctttgcggcg gtcacagttc gcgcatggga cgggacaaag gcctcatcga
     5341 acgaaacgga attccgtggg ccagaatcat ctatgattct ctttccctta ttctaaagaa
     5401 tgtactcgtt tccatacgag aagaacaacg ggcgacttac gagaaggtgt tttcgtcgga
     5461 agttctgatt ggagattctg tggacacagt gcagggaccg atgcgtggac tcatcagctc
     5521 ctttttgaaa tgcgacacag acatcctgct ggttggctgc gatatgccag atctgagaca
     5581 gagcctgagc catcttcttc aactacgcga agaaaatcca gactacgatt gcctggccta
     5641 ccggactgcg agagtggagc ctctatgcgc gatttacgga aaaaacatcc tgaagcaact
     5701 acgcgatcat catccagaaa cattcagcct ggaacgcttg ctcgaacgat ctcgcactct
     5761 cttcattcaa gcagacgaaa atcagacggc tgaactcaga aactacaatg aaccaggtcc
     5821 ggcactatga tcacaggttc cccattccca gcttccgtcc gcgaaatatt cctttttcca
     5881 gatgggcact tcatgtttga tgcgatgaat gattctctgg ttggcctcat aggcctccgc
     5941 gcgatgtgca gcagaagtaa ccacaaccac tgcgatttct ccaagcccaa ctctgccgat
     6001 tctgtgcacg gccatagctt cgtataaccg cagtttgtcg ctctcttcgc gaagaatctg
     6061 gctgatcatg atgggcgcaa gcttctggtg cgcgctgtat tccagatgat tcacctctct
     6121 tccgccgtgg tgatttcgaa ctcgacctgc aaagatcacc tgggcccccg cgcgcaaatc
     6181 ggactgcttc gtaaaaagtc ccagatcgat gggctcatct gtgagcagac gcggatgaat
     6241 tgattgtgta gcctgtgaac cggtcatcat caacctccgc tcatgggcgg aaagacgcaa
     6301 attgtggccg gaacttgctc ccctttgccc agaactcgat cgtccgtcgc gcagcaggaa
     6361 acaccgagca gagacgcagc ctgtggattc agatctgcca gtgcggtcag tacgtctgcg
     6421 gccgatgccc cttctttcac agacaaatcc agccattcac caaatgtatc cctgagggat
     6481 gcgaaaaatt ggatcctgat ttccatgctt atcctcccag ctcctgcatg gatttctcgc
     6541 tccctacaaa tcggactggc tgctttctcg agagtgctgt gcgaagagcg ttctctagtt
     6601 ccgatttctg cagcccgttt atattaatac ccctcgcgtc actgatacaa ccgtatatgt
     6661 tcccacgcgc atccattcga agacggttgc agtcagagca aaaaggcatg gattcatttg
     6721 cgatcactcc gaacatataa ccgtccgtaa ggcgaaagta atgagcggta gatgatttct
     6781 cggtaggtac gggggtaaaa gattcttcgg ttgcgattgc atccaggatg gtttgctcct
     6841 tcacgagcag agaccttgca tctttacgca atggtcccat agccatgagc tccagatatc
     6901 gtacggttac acccagagat cttgccaatt taaaaaccgg caaaatctcg ttttcattct
     6961 gtccgcgcac cagtgtgcag ttgatcttca ccggcaacaa ggcacgggcc acgtcaatcc
     7021 cgtgcaagac tccagcgaat ccgccccttc gtgccatgcg acgaaaccct tcttctcctg
     7081 cggcatccag agaaacattc aaagaagtca atccggcatc tcgcagaggc agaaccagct
     7141 cccttaaatt ccaaccgtta gttgtaaggt gaatcgaatt gagtcctgcc ttcccgaaca
     7201 gagccaccag atctacgagt ccagggtgaa gcgtgggctc tcctcccgtg attcgaatct
     7261 tcttaatgcc agcgatttct ataagcggca tggcgcttga gaaaatttcc tccgcactgc
     7321 gaaaagggcg ggagtccgct ttgaaaggag agtctcccgc gcaatagaca caggcgaagt
     7381 tgcagcgatc tgtaacgctc aatcgtaacg tcgcaaagga tcgtcccatt gcatccgcaa
     7441 tcaatggtcc cgcaccagac acgatcaaag attcagccaa tgattgcttt gtcatgatca
     7501 ccacagaaag ggaatgtatt caacggggct gccaaccgga atactggagg actgtggagg
     7561 cataacagcc agaccagtag cccgcacaga tgcaaatgcg tctccgcttc cgttatgcgg
     7621 cactgattct agagtatcgt cctctcgcag agtcactcgc agatattcct gaagcttgtg
     7681 attctttgca atcgatttcg caagtcgttt gaaaggatac tgttctcttc ttaaacccag
     7741 gatcttgcgc agaactggtt ctacgaataa ccgggcagtt acgtgagtag aaactggatt
     7801 cccgggaagc gcaattacca tacaggaatc aaaaaagccc gcccatactg gttttcccgg
     7861 acgaatggac actccgtgaa acaatttctc cacaccgcat ttctccatca caccaggcaa
     7921 atagtctgct tctcccgcag aaactccacc ggacaataca agtaactcag aatcaagcga
     7981 actctcgagc cgttttttaa gctctgcagg atcatctgcc acgtgtgaga cagtggcagg
     8041 atgcaaggcg tagtacgaga acaaagacgc aatggtgtaa cgattgctgt ttcggatttc
     8101 cacagcgcga ggaactgcac ccggttccac aatttcgttt ccagttgtga tcaaatgaat
     8161 tcggggagca agcactctca tactatcctg tccacaggaa gcagccgcgg cctgaacgga
     8221 tgcgtctatg attcgtccag ccagaacgat ctgatccccc ggctccgcgt cttctcccct
     8281 cttcgctata ttctgccaga ttttttttgc gggagagaga accacatgat cccccgtcac
     8341 ctggctttct tctaccttga ctacagtgtc cagatcctca ggaaccgcag caccagtcat
     8401 gattcgaacg gcactaccag gtggcacaga tgtcggtgga tcatcgcccg ccagaatagt
     8461 tgcaaaaata gacaaactgc gtgaagcaat gagatcatca aacctgactg cgaacccgtc
     8521 catggcggat ctatgaaagg gaggataatg acgatccgcc agaatgtcct gggccagaac
     8581 gcggcgggca gagtgttcga gcaaaacaga ctcagttctc acagttgtag gagttcgatc
     8641 gagtaacaac tgcaaggcgg aagcagaatc aatcatcatg cctgccgccg cgcagaattc
     8701 cagcaccgtg aaacacagcc ggaaaaattg catccatgct ttctgtcacc ccacgggtgc
     8761 ttcctggaaa cgctacaatg atagactgat ctgcggtgcc cgcgacggaa cgagaaagca
     8821 tagcaacagg agtcctctgc ccaccatgaa ctcgcattgc ctctgcgatt cccggaacag
     8881 gtctttgtat gagtttctta agtgtttcta ccgtgacgtc gcgcggacca agtccggttc
     8941 cgcccgtggt aaaaatgaag ggaatctttt ctgcaatcca cttctccacc cactgctgga
     9001 tctgttgcgg ttcatccgcg accactctgt agtccgccac tgttactccg agagacgtca
     9061 ggcgatcttt gatggctgtc ccggacccgt ccggtttctt gcctgcagct actgtatcag
     9121 aaacagttag cactgctgcc gttgtgcctt ccttgatgcg attccatttt tccgatttgc
     9181 cgcctgtctt cttgtccaac tgaattttct cgatgctcat ttctgcatcg taaggcttca
     9241 gtagatcata caaattcaaa gcagccacgg aggccgcggt gagtacttcc atctcgagtc
     9301 cggtgcgacc aatggatttc gcctccactc tcactatgat agaaccgggc tctttttcaa
     9361 aagtcacaga cattccatct atagacagag gatgacagaa cggaatcaac atgggcgtct
     9421 gcttggctcc aatcaatgcc gccgtcttcg aaatctcgta aatgtttcct ttgggagtaa
     9481 ttcccctggc aacaatttcc tctacgtccg gagaatactt cagccgcgcc gatgctgttg
     9541 caattctgag tgtattgatt cgagccgcga tgtccttcac aacgcccagc gagacaaatc
     9601 accggccgct gtcaaacgaa cgcgcaacta tcgcactttc tgctcagcgg gcaaagattc
     9661 cgcattcgat gcagtttcat gaaccatgac aaaaacagag ccgtccttaa gctgcacagg
     9721 atacacattc acagaatagc cggcatcgct gatgcaattc cctgacttca gggaaaacac
     9781 tttcttgtgc atgggacagg caacaacggg ctctcctttc ttgtctccaa tgattccacg
     9841 accgagtact gcttcgttct ggtgcggaca aacattgtcg catgcatacc atgtgtctgt
     9901 ttcattctca aaacgataca ctgcaatctg acgatttccc agcttgacta caactccgcc
     9961 gtcgcgagga aaatccgctt ccgcgccagc gcgcatccaa ttcaaatttg cgttgttcat
    10021 attttatacc cctgccccga cgggctcagc gggtttcttt tgtccgcgtt catattcaaa
    10081 gcgaatcgtt tcgtctgtct catccgtgtt tacaaaaggc cggaatcgcg cgcgaagcgc
    10141 cgggctcgac acgacttcgt tccattcgca tttgtagttc ttcaccagaa gctccatttg
    10201 ctgttcgagt tccgcgcaaa tccccagaga atcatcaatg atcacgct
//
LOCUS       DAOQJL010000072        10202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3431_length_10202_cov_4.462403, whole genome shotgun sequence.
ACCESSION   DAOQJL010000072 DAOQJL010000000
VERSION     DAOQJL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_10202_cov_4.462403"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2370)
                     /locus_tag="PLG78_04945"
     CDS             complement(58..2370)
                     /locus_tag="PLG78_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNE22487.1"
                     /translation="MYAHSILAAALKPDAFRFLFHPEPVVDFPEGAPVERVEMEKLAI
                     HKDAQGVILAKKRDTKLFVDPVTGSFHITLPDETRLSGTVQTGKFGPPSFVVRLMPGD
                     SVFGFGCATGGLVKAGSFELKTIDTIFYSIDQASYSAFPFFVVRRGSSFIGIFLNCIR
                     PTRVEVDLEGKTRPEGPEVRFDIQSTEAVPIDVFVMSGNLRQVLAAYARITGRPFLPP
                     LWALGYHQSRWSYRTQKRVMELAERFRALDVPCDAIHLDIHYMDKYKVFTWDKSTFPD
                     PKGMNESLDKLGVKTVAIVDPGVALEEEYDTYESGRNEDAFCRDSMGNYFKGKVWPGT
                     TVFPDFTLENVRKWWAHQHAVLFRNGVSGIWNDMNEPTWKMGKKTDPLLEDVHFKNHS
                     QAEMRNLYANLEAEATNLAFEKFKKGERPFVLSRSGFSGIQKYACMWTGDNHSSWQQM
                     KENLHMVLNLGLSGVPFSGADAGGFGSGKGMLAIIKWRRDPELFARWMELASLMPFFR
                     AHTVLFGRDQEPWSFGESVLAICRKHIRRRYRLLPYIYNLFHEASEDGSPIVRPLFYE
                     FPNVKDEDCEDQFLLGSSILAAPVFERKAANRRVNLPDAGEGWFDFDTGVVRDAGRHT
                     YSTRPGMYPLLVRAGTALPMAVVGRNALDTMKSPLAFEIFPGTQISGKCILDDGISAD
                     GRNARIEVSGKKDRSGSIQLEVQVRENGFSPFQKTIRLRLPGNYRTATQKGKRIEGVP
                     FDLTQEDRVGTAMQFEIPLENQKIEFDFRA"
     gene            2459..3454
                     /locus_tag="PLG78_04950"
     CDS             2459..3454
                     /locus_tag="PLG78_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE22488.1"
                     /translation="MRRKIALFSTLGVFVILGAVIIPRPGYHYQPHSLPADFDSFYAM
                     KLKQSKEKGARPGCEEKLNRFGPKTEYAILYLHGYGASRAEGEESIDRFANKLHANTY
                     YVRLPGHGTNMEDQASVSYTDYLDESEEALQMMPALGDKIIIVGTSMGALLATHLAAE
                     HPDKLYALIIASPFYEFVRKEANIASVPGGGKLAEVFGGGKIRDVTKKPDDVKDKRSP
                     GFENYWYEKQYFSSIQSLARLKKAVSGEHTFGRVTAPVLMIYYYKNENEQDTAASVPA
                     MLEGFHQFGKFTRPSPYNRSVAVADGDHIMFSKWIPTDKDLIDKELNRFIDDLKK"
     gene            3451..4176
                     /locus_tag="PLG78_04955"
     CDS             3451..4176
                     /locus_tag="PLG78_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE22489.1"
                     /translation="MKTLIIIPAYNEESTIYEVVTKTLKHADVSVTNDGSRDRTPEIL
                     RTIQAECSSGKHAHKLNVVTHEKSTHIPKGLQDGFRYGVENGYDQFITMDAGMSHDPD
                     AIPDFLAADSSLDVVIGARKQVENVPFYRRVISRCAALVVNYALTPSYFRLGPGLSDC
                     TSGFRRYSHRAGLLISATELKSKAFDFHMEALALCVRAGMKPGEIAIRYVFSNSSFNS
                     RVLKQAMKFGMYLISTKGKAAAS"
     gene            4173..5447
                     /locus_tag="PLG78_04960"
     CDS             4173..5447
                     /locus_tag="PLG78_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22490.1"
                     /translation="MKSVNQAGLLTALRSVRAAPLLFALYAGTACLAIVARYDFDSTA
                     LIRFGHRYIAQNQDLTPTSAIRFRGNEANGGNGYDGQIFYYYASTMWKKGQWPVGFSN
                     AYRAPRAGYPFLASLFSWMGNRGTAYGMILVQVLSIVGGLICFWHLLPDERRSWTLLL
                     AVSPFVLQSFAFLVSDAVMISLAIIGIYFSRKNSVAASLLAWIFFSLAVMTKESSLFI
                     LFPYGLWALLRKDWKLCVVVAGSLVPMVCWQLYLFRAHGMVPASILSTFLSPLLGIRG
                     VASQTADLLRMKPFPIVEIFKVSAKWLLILLLPVSAWTALRGFKLADLQKISTPFRLA
                     ALFVVLSICIADFQYFWGIFENVGRMFTALVPAVILARSETKTDTSWKAFVLLLCALS
                     FLVWARICFLSPVFPFDHYIPYSGPVYPAPPL"
     gene            5517..6380
                     /locus_tag="PLG78_04965"
     CDS             5517..6380
                     /locus_tag="PLG78_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE22491.1"
                     /translation="MSISQVDLRQTRLPIQPQIISALMQLTDDSEVGFRDLEKIIKAD
                     ENISTLILKAANSPLYSRGKEIRSIQIAISLLGFRVIRTLATVASSQGMFQSGNYARF
                     RKYVSEHAVITGVIARSIAVKLKLKNLEEDAFVGGLLHDLGKVIMNSLDRSHYIEVIH
                     LVERGVPFSQAEKQIFGFSHRDIGEKAAMTWNLPQIYRWILSTHGLDALTPPEGMDES
                     SRALVQIVACANYLARLYGYGAPLSGIEHEISKFFDALGMDSDTRSFYLSEFRENIPK
                     DEFYNFFITIL"
     gene            6386..8083
                     /locus_tag="PLG78_04970"
     CDS             6386..8083
                     /locus_tag="PLG78_04970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22492.1"
                     /translation="MLQQSTLEFEIRLYDLFSEEISALRDAVNRLADAVDPVSLRGPL
                     NAIVYELCGNALKALYKRAYSKYVVAKLGLDDLPYHDWLKLFRAEIEAHRAENFAHLC
                     RQENIYIKASGKFAGDVFRIQIENDGAPSEIEMSRIHLALERAKETMSLAAYFDDEDA
                     YQEGAGLGLPMVMVSLRGMGVDPANFQIFTQGDTTVARIDVPLNLFGNARSEPLRLLR
                     QNKELLAITWNLFQQIDLSAVRFDLDGHILAVSRSMLKRLAIPLETPDMFKTLVPQRF
                     VAELFRGPQGIRVTQKFLNHRVYLENLSRTERILFNVSGYVTDKGVVSTLWQEIAISD
                     SAAPKLNEGSLIGNLKFHNIIEPYIPQLVLMKARETVELGRTELPEEVRDQTIFFADL
                     AGFTEMSESMRPHVVVEMLNMALSIMVRHIKEAGGSVDKFMGDAVMAVFADPLPACKA
                     ALEIQNTFAEVNRFRIEAKEPPVRARIGIHCGNVIMANIGTDERKDWTAIGDTVNTAS
                     RIEKNAPVGSILISDRVFERVKDDLRVTQSYRIKVKGKAKEISVYELEAMNSAPADS"
     gene            complement(8088..8966)
                     /locus_tag="PLG78_04975"
     CDS             complement(8088..8966)
                     /locus_tag="PLG78_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE22493.1"
                     /translation="MKRLLAVILPVALLAAGAVYFSSAILYTGKYACSEEHFVYCGDP
                     SSLGLGFENIEFRTSDDLMIRGWWIPGRAGAPAIVVVHGRGVDRHEGLRFAPALHSAG
                     FNVLYIDLRNCGQSQKAFSSMGYYERRDVHAAVDYAVSRGMHSIGVFGFSMGAATSIM
                     AMSEDLRIRAGIFEGGFASFSSAISDAAWQDYKIPRYPLLPLVGALVSLRARMNTEEI
                     VPVKMISEISPRPVFIIHGTADVRVLPHNGEELYAAAREPKWYWVVPGGRHTRAWQAD
                     RAQAEAKVTEFFLKYL"
     gene            complement(8963..9571)
                     /locus_tag="PLG78_04980"
     CDS             complement(8963..9571)
                     /locus_tag="PLG78_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE22494.1"
                     /translation="MSVDAVIFDLGKVLLDFDLEKCFSVLSRSTGVSADRVRSAMFSE
                     KMVEFERGVLSPEQFFEHVSIQIDGAADGPTIREAFDDIFTAMNENIRLAEMLSQRVP
                     VGLVSNTNQSHSSFVEKNYKFFHIFKAKIYSHEHALRKPEPDIYHRAIDLLGVPAERT
                     LFIDDLEKNIEGARAIGMAVVHLPPGKPLLPELQAAGVQGIA"
     gene            complement(9589..9909)
                     /locus_tag="PLG78_04985"
     CDS             complement(9589..9909)
                     /locus_tag="PLG78_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22495.1"
                     /translation="MRTFLFIAIFAGFTTALLAREIQFDVTNETAQKITTLTALEKGA
                     NRRIPLFVGKGIKPGITATVVWATFAEEGDCLWTVQAKFENGTLSKPVAVDFCKQKEF
                     SIKE"
     gene            complement(9981..>10202)
                     /locus_tag="PLG78_04990"
     CDS             complement(9981..>10202)
                     /locus_tag="PLG78_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE22496.1"
                     /translation="VSFNVDGVHPHDVGSILDEEGIAVRAGHHCCEPFMRKLDISGTV
                     RASFYLYNGPEDVDALIRGIKRVKEIFKK"
BASE COUNT         2599 a         2649 c         2456 g         2498 t
ORIGIN      
        1 gagaaatggt actgtaagtt gtcttgtttt gcggagcatt tcgcgagcct ccatcgctca
       61 ggctcgaaaa tcaaactcta ttttctggtt ttcaagtgga atttcgaact gcatggcagt
      121 tcctacccgg tcttcctgag tcaggtcaaa gggcacacct tcgattcttt tccccttctg
      181 agtggcagtc ctgtagttcc cgggcaaccg caggcgaatc gtcttctgaa aaggcgaaaa
      241 cccattttcg cgcacctgca cttcgagctg aatggaaccg ctgcgatcct tctttcccga
      301 tacttcaatg cgagcatttc gaccgtccgc tgagatccca tcgtccagaa tgcattttcc
      361 ggagatctga gttcccggga agatctcaaa ggccagaggc gatttcattg tatcgagtgc
      421 attgcgtcca actacggcca tgggaagggc tgtgcccgca cgaacgagca gcggatacat
      481 accgggccgc gtggaatagg tatgacgtcc cgcatcgcgc acaactcctg tgtcaaaatc
      541 aaaccagccc tctcctgcat ccggcaggtt cacgcgacga ttggccgcct tgcgttcgaa
      601 aaccggcgct gccagaatgg atgatcccag caagaactga tcttcgcaat cctcgtcttt
      661 gacattcgga aattcataga acagtggacg aacgataggg ctgccatcct cgcttgcttc
      721 atggaataga ttgtatatgt acggtaatag tctatatcta cgtcgaatgt gcttgcgaca
      781 aattgcaagg acgctttcgc caaaactcca tggctcctga tcccgtccga aaagcacagt
      841 gtgcgcacga aagaatggca tcaaggatgc aagttccatc catcgtgcga agagctctgg
      901 atcacgtctc cacttgatga tggccagcat tcctttgccg cttccaaatc cacctgcgtc
      961 cgctcctgaa aacggaacac cactcaatcc gagatttaaa accatgtgca ggttttcttt
     1021 catctgctgc caggaggaat gattgtcgcc cgtccacatg catgcgtatt tctgaattcc
     1081 agagaacccg gatctggaga gaacgaaagg acgttctccc tttttaaact tttcaaatgc
     1141 aagattggtg gcttctgcct cgaggttcgc gtacaggttg cgcatctctg cttgcgaatg
     1201 atttttgaaa tgaacgtctt cgagaagcgg atctgtcttc ttccccatct tccatgtggg
     1261 ctcgttcata tcgttccaga ttcccgaaac tccgttgcga aagagaacag catgctggtg
     1321 cgcccaccat ttgcgcacat tctcaagagt aaaatcggga aacacagtgg ttccaggcca
     1381 gacttttcct ttgaagtagt tgcccatgga atcacggcag aacgcatctt catttcttcc
     1441 gctttcgtat gtatcgtact cttcttcgag tgcaactcct ggatccacga tcgcaacagt
     1501 cttcactccg agcttatcga gtgattcgtt cattcctttt ggatccggga atgtactctt
     1561 gtcccaggtg aatactttat atttatccat gtaatggata tccaggtgaa tggcatcgca
     1621 tggtacatcg agtgcgcgaa agcgttccgc aagttccatc actcgcttct gtgtgcgata
     1681 gctccatcta ctctggtgat atccaagagc ccaaagaggc ggaagaaacg gacgacccgt
     1741 aattcgcgca taagcagcca gaacctggcg aaggttcccg ctcattacga acacgtcaat
     1801 cggcactgcc tctgtagatt gaatatcaaa ccgaacttcc ggtccttccg gacgagtctt
     1861 tccttccaga tcgacttcta ctctcgtggg gcggatgcag tttaagaata tgccaatgaa
     1921 cgaagagccg cggcgaacta cgaagaatgg aaatgcggaa taggaagcct gatcaataga
     1981 atagaaaatg gtatcaatgg tcttgagttc aaacgatcct gctttgacca gtccgcctgt
     2041 cgcacagcca aaaccaaaca ctgaatcacc aggcatcaaa cgcactacaa aggaaggtgg
     2101 cccaaacttt ccggtctgca cagtgccgct cagtctggtt tcgtctggca aggtgatgtg
     2161 gaatgagcca gtcactggat cgacaaacag ctttgtgtcg cgcttcttcg ccaggatcac
     2221 tccctgcgca tccttgtgaa tggccagctt ttccatttcg actctttcga caggagcccc
     2281 ttctggaaag tccactacag gctccggatg aaacaggaat cgaaacgcat ctggtttcaa
     2341 cgcggccgca agtatgctgt gagcgtacat tcgctttatt ttcggtccgc gcgaccccga
     2401 aaatcaatgc aggcgggctt tttggttgcc atggcacgcg cgcaggcccg cctgttgtat
     2461 gcgcaggaaa atcgccttat tctccactct gggagtattt gtcatccttg gggcggtcat
     2521 cattcctcgt ccaggctatc actatcagcc tcattctctc cccgcagact ttgactcttt
     2581 ctatgccatg aagctcaagc agagtaagga gaagggtgct cgtcccggct gtgaagaaaa
     2641 gctgaatcga tttgggccaa aaacagaata tgcaatcctt tatcttcatg gctacggagc
     2701 atccagggca gagggagaag agtcgatcga ccgattcgca aacaaacttc acgcaaatac
     2761 gtactacgtt cgtctgcctg gacacggaac gaacatggaa gaccaggcct ctgtatctta
     2821 cacagattat ctggacgaaa gcgaagaggc actacagatg atgcctgcgc tcggagacaa
     2881 aatcatcatc gttggaacca gcatgggcgc tcttctagcc acacatctgg cagcagagca
     2941 tccggataag ctctacgctc tcattattgc ttcccctttc tatgagttcg tgaggaaaga
     3001 ggcaaatatt gcgagcgttc caggcggtgg aaaactcgca gaagtatttg gcggcggaaa
     3061 aattcgcgac gtaactaaaa aaccagacga cgtgaaagac aaacgcagcc cgggctttga
     3121 aaactactgg tatgaaaaac aatatttctc ttccatccag tctctggcaa gactcaaaaa
     3181 agcggtgtcc ggtgagcaca cattcggtcg agtcactgct cctgtattga tgatctacta
     3241 ttacaaaaat gaaaacgaac aggacacagc agcgagcgtt ccggctatgc tagaaggatt
     3301 tcatcaattt ggtaagttta ccagaccatc tccttacaac cgctctgttg cagttgcaga
     3361 cggagatcac atcatgttta gcaaatggat tccaacagac aaggatctga ttgataaaga
     3421 actcaatcga ttcatagacg atctcaaaaa atgaaaactc tcatcatcat ccccgcatac
     3481 aacgaagaat cgactattta cgaagtcgta acaaagactc tcaaacacgc ggatgtatcc
     3541 gtaaccaatg acggaagccg tgatcgcacg ccagagatcc tgcgcaccat ccaggctgag
     3601 tgcagcagcg gaaagcatgc tcataaactg aacgtagtaa cgcatgaaaa atcgactcat
     3661 atcccgaagg gcctgcagga cggcttccgc tatggagtag aaaacggata cgatcaattc
     3721 atcaccatgg acgctggaat gtctcatgat ccggacgcaa tcccggattt cctcgcggcg
     3781 gattcgtcac tcgatgtggt gataggcgcg cgcaaacagg tagagaacgt gcctttttac
     3841 agacgtgtca tctccaggtg cgccgctctg gttgtaaact acgcactcac tccctcttac
     3901 tttcgcctgg gtcctggact ttccgactgc accagtggat ttagacgtta ttcgcacaga
     3961 gcgggacttt tgatttccgc aacggaatta aaatccaaag cgtttgattt tcatatggaa
     4021 gctctggcat tgtgcgtgcg cgcaggaatg aaaccgggcg aaattgcaat ccgttatgtc
     4081 ttttccaatt cttcctttaa cagccgcgtt ctcaagcagg ccatgaaatt cggaatgtac
     4141 ctgatctcca cgaaaggaaa ggctgcggca tcttgaaatc cgtgaaccag gcaggccttc
     4201 tgactgcact gcggtctgtc cgtgcggctc cactcctctt cgcattgtat gcagggactg
     4261 cttgccttgc gattgtcgcg cgatatgatt ttgatagcac agcgctcatt cgctttgggc
     4321 atagatacat agcgcaaaac caggacctga ctcctacctc tgccattcga tttcgcggga
     4381 acgaagcaaa tggcggcaac ggatacgatg ggcagatctt ctactattat gcgagcacga
     4441 tgtggaagaa aggccagtgg cctgtcgggt tcagcaacgc atacagggcc ccgcgcgcag
     4501 gttatccgtt tcttgcgtcg ttgttttcgt ggatgggaaa ccgcggcact gcatacggta
     4561 tgattctggt tcaagtactc tccattgtgg gaggattgat ctgcttctgg cacctgctgc
     4621 cggatgaaag aagaagctgg actctacttc ttgcggtttc tccttttgtt ttgcagtcat
     4681 ttgcgtttct agtcagcgac gcggtgatga tttcacttgc gatcattggg atttactttt
     4741 cgcgaaaaaa ttctgtggcc gcgagtctac ttgcgtggat ctttttttcc ctggctgtta
     4801 tgaccaagga atcgtctttg ttcatactgt tcccgtacgg actctgggca ctgttgcgca
     4861 aagactggaa attgtgcgtc gttgtggctg gctccctcgt acctatggtc tgctggcagc
     4921 tgtatctatt cagggctcat ggcatggtgc ctgcaagcat tctgtctacg ttcctttctc
     4981 ctctccttgg aatcagagga gttgcttccc aaacggcgga tcttttgaga atgaagcctt
     5041 tcccaatagt tgagatcttt aaagtatctg ccaaatggct tttgatcctt ctcctcccgg
     5101 tatccgcctg gacggctctc cgcggattca aacttgcaga tctacaaaag atcagcactc
     5161 catttagact ggccgcgcta ttcgtggtcc tgagcatctg cattgcggat tttcagtatt
     5221 tctggggcat ctttgaaaac gtaggaagaa tgttcacggc cctcgtgcct gcagtgattc
     5281 tggccaggtc agaaacaaag acggacacat cctggaaggc ctttgtactt ctcctttgcg
     5341 cattgtcttt cctggtgtgg gcccggattt gttttctttc tcctgttttc ccgttcgatc
     5401 attacattcc atactctggt ccagtctacc ctgctcctcc actatgattt tggttgacgg
     5461 actcagccgc aagaaccaaa ccttcgaagc gtgcatctta aaggtatgag gaatcaatga
     5521 gcatctccca ggtagacctc agacaaacgc ggctccccat acagccccag atcatcagcg
     5581 ccctcatgca gctgaccgat gattcagaag ttggcttcag agatcttgaa aagatcatca
     5641 aagcggatga aaacatcagt actctgattc taaaagccgc caactcgccg ctttactcca
     5701 gggggaaaga aatcagatcc attcagattg caatttctct gcttggattt cgagtgattc
     5761 gcacccttgc aacggttgcc agctcccaag gaatgtttca gtcagggaac tacgcacgtt
     5821 ttagaaaata tgtgagcgaa cacgccgtca tcacgggagt gatagcacga agcatcgccg
     5881 tgaaactgaa gttaaagaat ctggaagagg atgcatttgt aggaggactc ctgcatgacc
     5941 tgggcaaagt aatcatgaat tccctcgatc gatcgcatta cattgaagtg attcacctgg
     6001 tagagcgagg agttcctttt agccaggcag aaaaacagat ctttggtttc tctcatcgcg
     6061 acattggtga aaaggcggcc atgacctgga atcttccgca gatctaccga tggattctca
     6121 gcacccacgg actcgacgct ctgactccgc cggaaggaat ggacgaatca tcgcgagcgc
     6181 tcgtgcagat tgttgcatgc gcgaactatc tggcaagact ttatggctac ggcgcgcctc
     6241 tgtctggaat agaacacgaa atctctaaat tctttgatgc tctaggaatg gattcggata
     6301 cgcgatcctt ctacctctcc gagttcagag agaacattcc gaaagacgaa ttctataact
     6361 tcttcatcac gatcctataa tttatatgct gcaacaatcg acactcgaat tcgaaatcag
     6421 actctacgat ctcttctcgg aagaaatcag tgctttgcgc gacgcggtca atcgcctcgc
     6481 ggatgcagtg gatcctgttt cgcttcgcgg acctctcaac gccattgtat atgagttgtg
     6541 cggaaatgcc ctgaaagcac tgtacaaaag agcctacagc aagtacgttg tggccaaact
     6601 cggtctcgat gacttaccgt atcatgactg gctgaaattg tttcgcgcag aaattgaggc
     6661 gcatcgcgca gagaatttcg cgcatctatg cagacaggaa aacatttaca tcaaggcatc
     6721 cggaaagttc gcaggggatg tttttagaat tcaaatcgaa aacgacggag ctccatctga
     6781 aatcgaaatg agtcgcattc acctcgcact cgaacgtgcg aaggaaacca tgagcctggc
     6841 cgcttacttc gatgatgaag atgcatatca ggaaggcgcg ggtctggggc tcccgatggt
     6901 gatggtgagt ctccgtggaa tgggtgtaga tcccgccaat tttcaaatct tcacgcaagg
     6961 ggacaccaca gtggcgcgca tagacgtacc tctgaatcta ttcggcaacg cacgctcaga
     7021 acctctacgg cttctcaggc agaataagga acttcttgcc attacctgga atctatttca
     7081 gcagatcgat ctatcagccg tgcgtttcga cctggatgga cacattctgg ccgtatcgag
     7141 aagcatgctc aagaggctgg ccatcccgct cgaaactccg gacatgttca agacgcttgt
     7201 tcctcagcga tttgttgcag aactgtttcg cggtcctcag ggaattcgag tcactcagaa
     7261 attcttaaac cacagagtgt atttggagaa cctttcgcgc acagaaagaa tcctattcaa
     7321 cgttagcgga tatgtgacgg acaaaggtgt agtgagtact ctctggcagg aaatcgctat
     7381 ttccgactct gcggccccca aactcaacga aggcagtttg atcgggaact taaagtttca
     7441 caacattata gaaccttata taccccagct tgtgttaatg aaggcccgcg agacagtcga
     7501 gcttggaaga acggagctgc ccgaagaggt gagagatcag accatcttct ttgcggacct
     7561 tgccggattc acagaaatgt cggagagcat gcgtcctcat gtggtcgttg aaatgttgaa
     7621 tatggctctg agcatcatgg taaggcatat caaagaagcg ggcggtagcg tggacaagtt
     7681 tatgggagac gctgtcatgg cggttttcgc ggatccactt cctgcatgca aggcagctct
     7741 cgaaattcag aatacgttcg cagaagtgaa ccgtttcaga atcgaagcta aagagccgcc
     7801 ggtccgggcg cgaattggga ttcattgcgg caatgtgatc atggcaaaca tcggcacaga
     7861 cgaacgcaaa gactggactg caattggaga cacagtcaat actgcgtcgc gcattgagaa
     7921 aaacgcacct gtaggatcca tcttgatttc agacagagtc tttgagcgcg taaaagatga
     7981 ccttcgagtc acgcaaagct atcgaatcaa agtcaaaggg aaagcaaaag aaatcagcgt
     8041 atacgaactt gaggccatga actccgcccc tgcagatagt tgatatccta cagatatttg
     8101 agaaaaaact cggttacctt tgcttccgcc tgagctctgt ccgcctgcca ggcgcgtgta
     8161 tgtctcccac ccggcacaac ccagtaccac tttggctccc gagcagcagc atagagttcc
     8221 tctccattgt gaggcaaaac tcgcacgtcc gcagtgccgt gtataatgaa tactggcctc
     8281 ggcgaaatct ccgagatcat cttgaccgga acaatttctt ctgtgttcat tctggcgcga
     8341 agggaaacca gagccccaac caatggcaaa agcggataac gtggaatttt gtagtcctgc
     8401 catgcggcgt cggagatcgc ggaagagaaa gaagcaaatc ccccttcaaa gatcccggcg
     8461 cgaattcgca agtcttctga catggccatg atggacgtcg ccgctcccat ggaaaaacca
     8521 aacactccaa tcgaatgcat gccgcgcgac accgcgtaat ccaccgctgc gtgcacgtcg
     8581 cgccgttcat aatagcccat agaactaaag gccttctggc tctggccgca attcctcagg
     8641 tcaatataca aaacattgaa gcccgcagag tggagtgcgg gcgcaaagcg aagaccttca
     8701 tgacgatcca cgcctcgacc atgaactacc acgatcgccg gagcaccggc acgtcccgga
     8761 atccaccagc cgcgaatcat caaatcgtcg gacgtgcgaa actcgatgtt ttcaaagccc
     8821 agaccaagag aagaaggatc cccgcaatat acaaaatgct cttccgaaca agcatatttc
     8881 ccggtataga gaattgcaga agaaaagtaa actgcacccg cagccagcaa cgccacgggc
     8941 aagatgaccg ccaggagccg cttcatgcga tcccttgcac gcctgcggcc tgcaattcag
     9001 gtagcaacgg ctttccgggt ggcaaatgga ctacggccat cccaatggca cgcgctccct
     9061 cgatattctt ttccaggtcg tcaatgaaga gagtacgttc tgcagggacg ccgagaagat
     9121 caatggctct gtggtaaatg tccggctccg gtttgcgaag agcatgttcg tgagagtaga
     9181 tctttgcctt gaatatgtga aagaatttat aatttttctc aacaaaagaa gaatgggact
     9241 gatttgtatt ggatacaaga cccacgggca cgcgctgcga aagcatttct gccagacgaa
     9301 tgttctcatt catggctgta aagatgtcat caaaagcttc ccggatggtg ggtccatctg
     9361 cagctccatc tatctggata gatacatgct cgaagaattg ctcggggctc aacaccccgc
     9421 gttcaaactc caccattttc tccgaaaaca tggcagatct gaccctgtcc gcagaaaccc
     9481 cggtgctcct cgatagaacg ctgaagcatt tctccaggtc aaaatccagc aggactttgc
     9541 ccagatcaaa gatgacggcg tctacactca tcacaccacg ttcggaacct attctttgat
     9601 cgagaattct ttttgcttgc agaaatctac ggccaccggc ttcgaaagtg taccgttctc
     9661 gaattttgcc tgcacagtcc acagacaatc gccttcctct gcgaaagtgg cccacactac
     9721 ggttgcggtg atgcctggct taattccctt tccaacaaag agtggaatgc gacgattcgc
     9781 gcctttctcg agtgccgtga gagtcgtaat tttctgagcc gtttcgttgg taacgtcaaa
     9841 ttgaatttcg cgagccagga gagctgttgt aaaccctgca aagattgcga tgaacaagaa
     9901 agttcgcatt ggagattccc ccgttttgat gtgatggttc aaataacgaa ctatcaggcc
     9961 aggccttcat gttgaaaaca ttatttctta aagatttctt tgactctctt gattccgcga
    10021 atgagcgcat ccacgtcctc cggcccattg tacagataga agctggcgcg aaccgtccca
    10081 ctgatgtcca gctttcgcat aaagggttcg caacaatgat gacccgcgcg aaccgcgatt
    10141 ccttcctcat ccaggatgct gccgacgtca tgaggatgca ctccgtctac attgaaagaa
    10201 ac
//
LOCUS       DAOQJL010000073        10141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3461_length_10141_cov_4.288420, whole genome shotgun sequence.
ACCESSION   DAOQJL010000073 DAOQJL010000000
VERSION     DAOQJL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3461_length_10141_cov_4.288420"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLG78_04995"
     CDS             <1..972
                     /locus_tag="PLG78_04995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE22497.1"
                     /translation="RSEPYPVHRISPGGRFSRHDLFDRFIQDRLAQFVPRHIVFGNVS
                     RGTMRTASIARDAGMHYSVFIPGTDLAKKLGFLNFLERRFVMGARNVFTLSRHLARSA
                     MEAGIPEDRIVVIPPGLELRWNRFKRFTVPQEIYEKIKGKTVLVSVGPFVQRKGLDTA
                     IEAMNRLKGPEADRRQLHLLLIGSGPEHAFLEELIRIRGLESYVTLTGFLPDDQLAAV
                     LGLAHIFVQPGTGGKDTESMGTSLMEAAYFGIPAIAGKTGVFEEIVRHGVTGYLFEPG
                     NVDELCRAILDIAGSDRMQLKLGRNARDIAGKEFDLARTCAAIQARI"
     gene            complement(998..2608)
                     /locus_tag="PLG78_05000"
     CDS             complement(998..2608)
                     /locus_tag="PLG78_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNE22498.1"
                     /translation="MRISILLCFLFATNLAAETIDEGGASHPGFTATLYGAESFRATD
                     STQDLRLAPAPASDAILQLRFEEPSSILKDSAGNFKVRANYVAQKNGPMGGFARFIKP
                     ENRIELSSPRELWPSDGDFSVEFWIEPVIFYSRNSIFKKVSLLGGQKRGMEIYLESGR
                     LKASFFDLFEDTDGQRHSITLSSHSALPGSRWSHVTLTYDASSGKLALFVDNQEQMVS
                     YAADATGVYRASFHPLDRSAIVIGENYFGSLDEFLITGSAISPEEHPRTRLPSVHISD
                     VSFRPTQQSGMAISEVYDAGNSSSGRFRYAADEPAGSSIQFFIRFSEHAFNSDAPDAG
                     SLKWKRIRNQTDNIGRFRFFQWKALFNADPTGSVSPVLRETTLTRFALEPPARPGGLR
                     AIEELSGGDSVCLEWRNVPEPEIESEGGYIVYYGLKPGEYLGHVRIQNGKPIRPLAPE
                     KMQLTKEEKRRTQYQSSVVMPELSGRTRFMLDNRTIEENIRGKKKGNMPFLRPDRTYY
                     FAVSAYTSQGESEQSKETVAVLRPAPDL"
     gene            complement(2632..3225)
                     /locus_tag="PLG78_05005"
     CDS             complement(2632..3225)
                     /locus_tag="PLG78_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22499.1"
                     /translation="MKEIAFAILLLQPMQTRAPGYFLMPALREGELTAFLKSPVMDRS
                     VLAQIASARENEARAILIQRRGEIRAEICATPESERPAAIAAIRKTVEDEHALYKKEI
                     SNYEAYIYRRMEKEIDPAYREQAVAARKDAFVLQRDRSSLIEQSWMKMLSAKDVCEQT
                     ELFSEKINLPYYLALFHYQSIFPAEMRSRTLLSDTPR"
     gene            3269..4333
                     /locus_tag="PLG78_05010"
     CDS             3269..4333
                     /locus_tag="PLG78_05010"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNE22500.1"
                     /translation="MQSSKIRLGVLCGGRSSEHEISLRSAANVLRAVDRTRYDVIVVG
                     IDRAGGWQIVSENALASKELLPVGKGAAYRAESLAGALPILMTCDVIFPVLHGKMGED
                     GTMQGFLRVLDLPFIGADVAGSAVGMDKWLMKRLLVEAGLPIGRYTAFRDVQSATANF
                     ATLRERFGMPLYVKPAAQGSSVGVHRVENETDFRSAVEDALRYDDVVLVEEAIAGREV
                     ECAVLGNEEPIASLCGEIIPKGGFYSYENKYLNEDGAAFEMPANLPGHVMDRLRKLAI
                     ETYRALDCAGLARVDFFLRPDESIIINEINTLPGFTSISMYPKLFELSGLPTPQLIDR
                     LVHLARDRHVKQTAKKIDRV"
     gene            4335..4889
                     /locus_tag="PLG78_05015"
     CDS             4335..4889
                     /locus_tag="PLG78_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22501.1"
                     /translation="MGGTIDLFDGLSSGLVPQGGGWIFVARFHKESNYAIFEIISYAN
                     VKRIEQSESGVEMFSRGLKAFVVYEPTNYPLRFMEPYLRDELEQIPLRWENLEVIEAS
                     SRDRVLVSKEPYMSLGSFVIETPRAGNFVYYVYEEEGRNSIRNLLLEIFKKDYNLEYR
                     AIERVLDLVDSNLGRMVEKRAESD"
     gene            4882..5619
                     /locus_tag="PLG78_05020"
     CDS             4882..5619
                     /locus_tag="PLG78_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22502.1"
                     /translation="MIDVRSAVIAAYRDHLSRRYTPQNVHRFEILADFPSQRIDEVRD
                     FFLARMYPEADRRLKLDLAFDKLGSVLKSPRKMMPLAGTAISSVFKLGTLIPAALRAG
                     LATFEGYIEIQRLESIMIKEAESRGIEDLSDEKAFAGIIAKIPDRDVQRFRRELTRLF
                     KSLANIPLLETTLEIMGNSIRIISARPDVYTQEELEGIQIGYELLAGGLELFRSLSPE
                     ETQMVLVGVDRIEMDWIERMRAQASVA"
     gene            complement(5634..6413)
                     /locus_tag="PLG78_05025"
     CDS             complement(5634..6413)
                     /locus_tag="PLG78_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE22503.1"
                     /translation="MSTIALPLPVKRQNSTKTRGKGKKATKSKSRSRQKIVYVEVVRK
                     RSKRWMDEFRSALRAEIANCMTHGMGVGLSLAGMSLLIASAAKAGDPWKIVSFAIYGT
                     CMVLLYLASTLYHGFLSPRAKKVFKVLDHSAIFLAIAGTYTPFTLVSMRGPWGWTLFI
                     IIWALAIGGIAYKMFFVNKYRLISVLIYILMGWLVCIAVKPMFQMIPLAGIIWIAIGG
                     IAYTAGTAFYASSFAMSHMIWHLFVLAGSICHYIAIYFYVL"
     gene            6522..6719
                     /locus_tag="PLG78_05030"
     CDS             6522..6719
                     /locus_tag="PLG78_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22504.1"
                     /translation="MQPNLQDILNAGIGLVLAADEAARNAISSAQKGFEDLKNKGAAD
                     YSDAAIKARSILQDTLRAVRA"
     gene            complement(6770..7093)
                     /locus_tag="PLG78_05035"
     CDS             complement(6770..7093)
                     /locus_tag="PLG78_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002152918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNE22505.1"
                     /translation="MEKQYNTMKEFWPFYLGEHRNKVNRILHFIGSTGVIAFAVCAAY
                     FMDWRYLVAMPLFGYGFAWVGHFFFEKNRPATFKYPFKSLGSDFVMYFYTWTGQIRRE
                     LDKIS"
     gene            7181..7957
                     /gene="proC"
                     /locus_tag="PLG78_05040"
     CDS             7181..7957
                     /gene="proC"
                     /locus_tag="PLG78_05040"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNE22506.1"
                     /translation="MDINLSTRKIGFLGMGKMGSAIARGLKNKHPNLNLTGYDPLIRS
                     DLVNAADSPESLESACDIVLLCVKPQEMKSALASFKGNKQYISIAAGLSTSSIQEMLG
                     GARHIARAMPNLNASIQMAATGIYSHDSELGLLARAIFECVGAVEMVEKEELLHVVTG
                     LSGSGPAFVFEFIDALAQGAVQAGLPYERALRLAAETLIGSASLVVLEKKHPLELRSQ
                     VASPGGTTIAGLAALEELGFRSAVMRAVVRAKERSEELGK"
     gene            7923..>10141
                     /gene="hrpB"
                     /locus_tag="PLG78_05045"
     CDS             7923..>10141
                     /gene="hrpB"
                     /locus_tag="PLG78_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNE22507.1"
                     /translation="MQKKEVKSWANSQTRDLPVTGAIPDLKQALASDKRVVLIAPPGA
                     GKTTVVPLELLDEPWLANRSILMLEPRRLAARGAATRMSSMISEKVGGIVGYRVRFEN
                     RVSERTRIEVVTEGILSRRIQNDPELEGVGLIIFDEFHERSIHADVGLALSLDVQSSL
                     RDDLRILVMSATLDGERISGFLSAPLIRAEGRTFPVENRYAGLRKTSPAHAASDAVSA
                     LISKSTGDILVFLPGSGDIRRAQELLEEARVDALICPLYGDLRQEEQDRAITPDPSGT
                     RKVILSTNIAETSLTIEGVTTVIDSGLERRLKLDPRTGLGRLETVRISKSSADQRSGR
                     AGRTGPGVCIRLYDEEEYRRMESHAKPEILEADLAPFALEMALWGTADLRFLDPPPAS
                     SLSAARSLLRSLGALDGDTITETGRAMCIPVHPRLARMIVEARSNAQAKIACDLAAIL
                     SERDVLRGSGSDLRTRLQALEDARSGSYGPAVHRGSISRALSLATEMRKYAVHSSKDT
                     DAVEPGKLLACAYPDRIARARDEKMERYLLSNGRGAVLPPGDSVRSLFLVAAETRGSG
                     ADSSIDLALPVSVESLRELFANQIEIQMETRLDKSGRVQSVRVEKLGALELSVTETSE
                     AVDAGSALLAEVLRQGIDRLSHFQETESLRNRVEFLRRNGEDLPDFSKEYLSANLGEW
                     LGPHIAVMRSISEVQKIDIQPILHGMLSHSQQSILRTHAPESMEVPSGSKISIDYSSP
                     "
BASE COUNT         2372 a         2473 c         2752 g         2544 t
ORIGIN      
        1 cgctcagagc catatccagt gcatcgcatt tctccgggtg gccgtttttc tcgtcacgat
       61 ttgtttgata gattcatcca ggaccgtctc gcacagtttg ttcctcgtca tatagtattt
      121 ggaaatgttt cgcgtggcac catgcgtacc gcgtcgattg cgcgtgatgc aggaatgcac
      181 tactccgtat ttattccggg gacggatctt gcaaaaaagt tgggttttct aaactttctg
      241 gaaaggcgct ttgtgatggg cgcgcgaaat gtatttaccc tgtccagaca cctggcgcgc
      301 agtgccatgg aagcgggcat tccagaagat cgcatcgtgg tgattccccc tggtctagag
      361 cttcggtgga atcgttttaa acggtttact gttccacagg agatctatga aaaaataaaa
      421 ggcaaaacgg tccttgtttc cgtgggccct tttgttcagc gcaagggtct cgatacagcc
      481 attgaggcca tgaacagact caaaggtcct gaagcagatc gcaggcaact tcatttgctc
      541 ttgattggct cggggccaga gcatgcattt ctcgaagagc tgattcgcat tcgcggactg
      601 gaatcctatg taacacttac tggttttctt cctgatgatc aactggctgc agttctcggg
      661 ctcgcgcata tctttgtgca gcctggaacc ggcggcaagg acaccgagtc catgggcact
      721 tcgcttatgg aagcggccta ctttggaatc cccgcgattg cgggaaagac cggtgtgttc
      781 gaagaaattg tgcggcatgg agtgactgga tatttgtttg agccaggcaa tgtagacgaa
      841 ttgtgtcgtg cgattctgga cattgcagga tccgaccgca tgcaattgaa gctgggtaga
      901 aacgcgcgcg atatcgcggg caaagaattt gacctggcac gcacctgtgc ggcgatccag
      961 gcgcgcattt aatcgcggcc cggaccgctt gcttctacta tagatctggc gcgggtctca
     1021 gcactgctac tgtttctttt gactgctctg attcaccttg cgatgtgtag gcggacacag
     1081 caaaatagta ggttctatcc gggcgcagga aaggcatgtt tcccttcttc tttcctcgta
     1141 tgttttcctc gatggtgcga ttgtcgagca tgaagcgcgt tcgtccggag agctctggca
     1201 tgaccacgga tgactggtac tgtgttctgc gtttttcttc tttcgttagc tgcattttct
     1261 caggagccag cgggcgaatc ggcttgccgt tctgaatgcg cacgtgcccc aggtattctc
     1321 ctggtttcag gccatagtac acaatgtagc cgccttcgct ttcgatctcc ggctcaggga
     1381 cgtttctcca ttccaggcat acggaatctc cgcctgacaa ttcttcaatg gcgcgaagcc
     1441 cacccggtct tgcggggggc tcgagtgcaa atcgtgtaag ggttgtttcg cgaagaacag
     1501 gcgaaacact tccagttgga tccgcgttga acaatgcttt ccactgaaag aatctgaatc
     1561 tgccaatatt gtctgtctgg ttgcggattc ttttccattt gagtgaaccc gcatctggtg
     1621 catctgaatt gaatgcatgc tcactgaatc gaataaagaa ttgaatggag ctgcccgcgg
     1681 gttcatccgc tgcatagcga aatcttccgg aagaagaatt gcctgcatcg tatacttctg
     1741 aaatcgccat accgctttgt tgtgtcggac gaaaggaaac gtcagagatg tggactgagg
     1801 gaaggcgagt gcgtggatgt tcttctggcg agatggcgct gcccgtaatg agaaactcat
     1861 cgagcgaacc aaagtaattc tctccaatta cgatggcgga tctgtcgaga ggatgaaagg
     1921 atgcgcggta gactccagtt gcatctgcag catacgacac catctgttct tgattgtcca
     1981 caaacagtgc gagctttccg gaactcgcgt cgtacgtcag agtcacatgc gaccatctcg
     2041 atccaggaag tgcggaatga ctcgatagtg tgatggaatg tctctggccg tctgtgtctt
     2101 caaacagatc gaagaaggaa gctttcagtc tgcccgactc cagatagatt tccattccgc
     2161 gcttctgtcc acccaggaga gagactttct tgaatatgct gtttctggaa taaaagatga
     2221 ctggctcgat ccagaactca acggagaaat cgccatctga tggccatagt tcgcgaggag
     2281 aagaaagttc gattctgttc tcaggtttaa tgaagcgagc gaatcctccc atgggtccgt
     2341 tcttctgcgc cacgtagttg gcgcgcactt tgaagttgcc cgcgctgtct ttgaggatgg
     2401 aggaaggttc ttcaaaccgg agctgtagaa tcgcgtcgct tgcgggtgca ggtgccaggc
     2461 gcagatcttg agtggagtct gtggctcgaa aggattcggc cccgtagaga gtcgccgtaa
     2521 atccgggatg cgaagcgcca ccttcatcga ttgtttctgc ggccaggttc gtggcgaaaa
     2581 gaaagcagag taagatggaa attctcaccc tatattatcg gtcaaacggg cttatctggg
     2641 agtgtcagaa aggagcgttc tggaccgcat ttcggccgga aaaatggact ggtaatgaaa
     2701 tagcgccagg tagtagggca ggttgatctt ctctgaaaaa agctccgttt gctcgcatac
     2761 gtcctttgca gaaagcatct tcatccacga ttgttctatg agactggatc ggtctctttg
     2821 caaaacgaat gcatctttcc tggctgctac cgcctgctcg cggtaggccg gatctatttc
     2881 cttttccatg cgtctgtaga tgtaagcttc gtagtttgaa atttctttct tgtaaagggc
     2941 gtgttcgtct tcgactgtct tacgaatcgc cgcaatggcg gccggtcttt ctgattccgg
     3001 cgtcgcacag atttcagcgc gaatttcccc tcttctctgg atcagaatgg cccgggcttc
     3061 gttttcgcgc gcagaagcga tctgggcgag cacgcttcgg tccatgaccg ggctcttaag
     3121 aaaggcggtg agctctccct ctcgcaacgc aggcatcaga aaatagcccg gcgcgcgggt
     3181 ctgcatgggt tgcaggagca ggatggcgaa cgcgatttcc ttcataaaaa aattgtttgg
     3241 taaaaccagg tgtttcggac tgtgacctat gcaaagctca aaaatccgcc tcggggtcct
     3301 gtgcggcggc cggtcatcgg agcatgaaat ctcccttcgc tcagcggcca atgtgctgcg
     3361 tgcggtggac cgcaccaggt atgacgtcat tgtagttgga attgatcgag cgggcgggtg
     3421 gcagattgta tctgaaaacg ctcttgccag caaagaactt cttccagtgg gcaaaggcgc
     3481 ggcgtatcgc gcggagagcc tggccggagc tctacccata ctcatgacct gcgatgtgat
     3541 ttttccggtt ctgcacggca agatgggaga ggatggcacc atgcagggct tccttcgcgt
     3601 actggatttg ccattcattg gtgcagatgt ggccggttcc gcagttggta tggacaaatg
     3661 gcttatgaaa cgactgcttg ttgaggcagg gctgcccatt ggacgttata cggctttcag
     3721 agacgtgcaa tctgccacag ccaattttgc aactcttcgt gaaagattcg gaatgccatt
     3781 gtatgtaaag cctgcggcgc agggatcgag cgtgggcgtg cacagagtgg aaaacgaaac
     3841 agatttccga tctgcggtag aagacgcgct ccgatatgat gacgtggttt tagtggaaga
     3901 ggcgatcgcc gggcgcgaag tagaatgtgc ggtccttggc aatgaagaac ccattgcttc
     3961 cctttgtgga gaaatcattc caaagggcgg gttctattct tacgaaaaca agtacctcaa
     4021 tgaggacggc gctgcctttg aaatgcctgc caatcttccc ggtcacgtga tggatcgtct
     4081 gagaaaattg gccattgaaa catatcgagc gctcgattgt gcgggtcttg ctcgcgtgga
     4141 tttctttctc aggccggatg aatcgatcat catcaatgag atcaatactc tgcccgggtt
     4201 taccagcatc agcatgtatc ctaaactttt tgaactatct ggtttgccaa cgccgcagct
     4261 gatcgatcgt ctggttcatc tggcgcgaga cagacatgtg aagcagaccg ccaaaaagat
     4321 agatcgagtt tgaaatgggt ggcacaattg acctatttga tggactgagt agtggcctcg
     4381 ttccgcaggg gggaggctgg atctttgtgg cccgttttca caaggagtcc aattacgcga
     4441 tcttcgaaat catttcgtac gcaaatgtga agagaataga gcaatctgaa tccggcgtgg
     4501 aaatgttttc gcgtggtcta aaggcattcg tagtctacga gccaaccaac tatcctcttc
     4561 gattcatgga accctacctc agagacgaac ttgaacagat tccacttcgc tgggagaatc
     4621 tggaagtcat agaggcttct tccagagatc gcgtgcttgt ttcaaaagaa ccttatatga
     4681 gtcttggatc gtttgtgatc gagactccgc gcgccgggaa ttttgtgtac tacgtttacg
     4741 aagaagaagg gcgtaactcc attcgtaacc tgcttctgga aatatttaag aaagattaca
     4801 atttggaata cagggccatc gaacgggtgc tggatctggt ggattcgaat ctgggccgga
     4861 tggtggaaaa gagggcagaa agtgattgat gtgcgcagtg cggtcattgc cgcgtatcgg
     4921 gaccatttgt cccgccgtta tactcctcag aatgttcatc gttttgagat tcttgcggat
     4981 tttccatccc agagaattga tgaggttcga gatttttttc tggcccgaat gtatccagaa
     5041 gcagaccgca gactcaagct cgatcttgcc tttgataagc ttggttctgt attgaagtcg
     5101 cctcgcaaaa tgatgcctct tgcaggaacg gccatcagtt ctgttttcaa gctgggcact
     5161 ctgattccgg ccgcgcttcg cgctggcctc gctactttcg aaggctacat tgaaatccag
     5221 cgactggaaa gcatcatgat caaagaagca gaatcgcgcg gaatcgaaga cctgtctgat
     5281 gagaaagcat tcgccggcat cattgcaaag attccagatc gtgacgtgca aaggtttcgc
     5341 cgcgaactca ctcgtctttt taagtctcta gccaatattc cgcttctgga aaccactctt
     5401 gaaatcatgg gcaactccat tcgcatcatc agtgcgagac cggacgtcta cactcaggaa
     5461 gagctcgagg gcattcagat tggatatgaa cttctggcag ggggcctgga actctttcgc
     5521 agcctttcgc ctgaggaaac ccagatggtt ctggtgggtg tggatcgcat tgagatggac
     5581 tggatcgaaa gaatgcgcgc tcaggcttct gtagcctgag caggccttat tccttacagc
     5641 acgtagaagt aaatggcaat gtagtggcag atgcttcctg ccagaacaaa caaatgccag
     5701 atcatgtgac tcatggcaaa gctagaagcg tagaacgcag tcccagcagt gtaggcaatt
     5761 ccaccaatcg cgatccatat gattccggcc agagggatca tctgaaacat tggtttgacc
     5821 gcgatacata caagccagcc catcaaaatg tagataagta ctgaaatgag tctgtattta
     5881 tttacaaaga acatcttgta agcaatgccg ccaatcgcca gtgcccagat gatgatgaac
     5941 agagtccatc cccaggggcc gcgcatgctg acgagtgtga acggagtgta tgtgcctgca
     6001 atcgccagaa agatcgcaga atgatccagg actttgaata ccttctttgc gcgcggagat
     6061 aaaaagccgt ggtagagagt ggaggcaaga tacagaagca ccatgcacgt tccgtagatc
     6121 gcaaagctta cgattttcca gggatctccc gctttcgctg cacttgcaat gagcagtgac
     6181 attccagcga ggctgaggcc aacgcccatc ccgtgggtca tgcagtttgc aatctccgct
     6241 ctcagggcgg atctaaactc atccatccat ctcttgcttc tttttctgac tacttccaca
     6301 tagacgatct tctgtctgga gcgggacttg ctctttgtgg ccttcttgcc tttgcctcta
     6361 gtcttagtgg aattctgcct tttgacaggc agaggaagtg cgattgtgct cattgaacca
     6421 taagaaccat cctcatgcca ggggttgcaa aattgggttg actatggtgc ggtgcattaa
     6481 aaatatggcg caacgcacta taagtgcgga ggagtacaga gatgcaaccg aaccttcaag
     6541 atattctgaa tgcaggcatt ggtctggttc tggcagcaga tgaagcagcc agaaacgcga
     6601 tatcttcagc acagaaagga tttgaggatc tcaagaacaa gggtgctgca gactactcag
     6661 atgcagccat caaagctcgt tccattctcc aggacactct tcgcgccgtt cgcgcgtaag
     6721 ttccttgcgc atctgccggc cttgcgcttg cgcgggccgg cagcgtagtt caggaaatct
     6781 tgtccagttc tcggcggatc tgtccggtcc aggtgtaaaa atacatcaca aagtcagaac
     6841 cgagtgattt gaacggatac ttgaacgtag cgggtctgtt cttttcgaag aagaagtgtc
     6901 ccacccacgc aaagccgtag ccgaacaaag gcatagcaac cagatatctc cagtccatga
     6961 agtatgcggc gcaaactgca aatgcaatga cacccgtaga tccgatgaag tgaaggattc
     7021 tgttcacctt attgcgatgt tcgcccaggt agaacggcca gaattccttc atcgtgttgt
     7081 attgtttttc catgaatgcc tcggtccaaa cttacaacga cctcctggat cagtccattt
     7141 tttttctgtg cagtgtcgtc gcctgcgtca tgcaggttgc gtggatatca atctgagcac
     7201 gcgaaaaatt ggctttcttg gaatgggcaa gatggggagc gcgattgccc gcggtctgaa
     7261 aaataagcat ccgaacctga atctaaccgg atatgatcct ctcattcgtt cggatctggt
     7321 gaacgctgcc gattctccag aatctctcga gtctgcatgt gacattgttt tgctctgcgt
     7381 caagcctcag gaaatgaaga gtgctcttgc gtcgtttaag ggaaacaaac aatacatttc
     7441 gattgcagcg ggtcttagca cttcctccat tcaggaaatg ctcggaggtg cgcgtcatat
     7501 tgcgcgggcc atgccaaatc tcaatgcgtc catccagatg gcggccactg gaatctactc
     7561 tcacgattct gaactcgggc tcctcgctcg cgccatcttc gagtgcgtag gcgcagtcga
     7621 gatggtggag aaagaagaac tgctgcacgt tgtgacgggt ctctcaggat ccgggcctgc
     7681 ttttgtattc gaattcatag atgctctggc gcagggcgcg gtgcaggcag gtcttccgta
     7741 tgaacgcgct cttcgtctgg ctgcggaaac attgattggt tcggcctctc ttgtggttct
     7801 agaaaaaaaa catcccctgg aactccgttc gcaggtggct tctcctggcg gaaccaccat
     7861 cgctggactg gccgctctag aagaacttgg ctttcgctcc gccgtgatgc gcgcagttgt
     7921 gcgtgcaaaa gaaagaagtg aagagctggg caaatagtca aacccgggac cttcctgtaa
     7981 ccggagccat cccggatctg aagcaagctc ttgcatcaga caaaagagtg gttctcatag
     8041 ctccaccagg tgcggggaaa accaccgttg ttcccctcga actcttagac gaaccctggc
     8101 ttgcaaatag atccatcttg atgcttgagc ccaggcgtct ggccgcgcga ggagcggcta
     8161 ctcgcatgtc ttcgatgatc tctgagaagg tgggaggaat tgtcgggtat cgcgttcgtt
     8221 ttgaaaacag agtgagtgaa agaacgcgca ttgaagttgt cacagaagga atcctttcgc
     8281 gcagaatcca gaatgacccg gagctagaag gagtcgggct cattattttc gacgaattcc
     8341 atgaaagaag cattcatgca gatgttggcc ttgctctttc tcttgatgta caatcttctt
     8401 tgcgcgatga tcttcgaatt ctcgtgatgt ctgcaacttt agacggagag aggatttctg
     8461 gctttctttc tgcacctctg attcgcgcgg aaggcaggac cttcccggta gagaaccgtt
     8521 atgcgggatt gaggaagact tccccggctc atgctgcctc tgacgctgtg tctgctctga
     8581 tttcgaaatc tacgggagac attcttgtgt ttcttccggg gagcggagat atacgccgcg
     8641 ctcaggagct tctcgaagaa gcacgcgtgg atgctttgat ctgtccgctg tatggagatc
     8701 tgaggcagga agaacaggat cgtgcgatca cacctgaccc gtccggtaca agaaaagtga
     8761 ttctttctac aaacattgca gagacttctc ttacgatcga aggagtgacc actgtcatag
     8821 acagtggtct ggagagaaga ttgaaactgg atccgcgcac cggtctcggt cgtctggaaa
     8881 cagttcgcat ttccaaatct tccgcggatc aaagaagcgg aagagccggt agaacgggcc
     8941 ctggagtttg cattcgactc tatgacgaag aagagtatcg cagaatggaa tctcacgcaa
     9001 agccagagat actcgaagcg gacctggcac cgtttgcgct ggagatggca ctctggggca
     9061 cggccgattt gcgattcctc gatcctccgc ctgcctcgag tctcagtgca gcgcgctcgc
     9121 ttcttcgcag tctcggagcc ctggacggag atacaatcac agagaccggg cgcgcgatgt
     9181 gcattcctgt gcacccacgc ctggcaagga tgattgtaga agcacgaagc aatgcccagg
     9241 cgaagatcgc ctgtgacctg gccgcgattc tttccgagcg ggatgttctt cgcggatctg
     9301 gatcggatct tcgcacaagg ttgcaagcac tggaagatgc gcgaagcgga tcgtacgggc
     9361 ctgccgttca caggggatcc atctcgcgag ctctttctct ggccacagag atgagaaagt
     9421 atgcagtcca ttcgtcgaaa gacactgatg cagtggagcc tgggaagcta cttgcatgcg
     9481 cttatccgga tcgaatcgct cgcgcgcgcg atgaaaagat ggaacgttat ttgctctcaa
     9541 acggaagagg ggcggtgctg ccgcccggcg attctgttcg atctctattt ctggtggcag
     9601 cagagaccag aggttcgggt gcggattcca gtatcgatct ggcacttcct gtttctgttg
     9661 aatcgctgcg cgaactcttt gcgaatcaaa ttgaaatcca gatggaaacg cggctggaca
     9721 aatctggacg cgtgcagagc gttcgcgtag aaaaactggg tgcacttgaa ctgtccgtga
     9781 cggagacgag tgaagcagtg gatgcaggat cggcacttct tgctgaagtt ttgcgtcagg
     9841 gaatcgatcg cctctctcac tttcaggaaa cagaatctct tagaaacaga gtagaatttc
     9901 tgcgcaggaa tggagaggat cttccagatt tctcaaagga gtatctttcc gcgaacctcg
     9961 gggaatggtt aggcccacac attgccgtta tgcgctctat ctcagaggta caaaagatcg
    10021 acattcagcc aatacttcac ggaatgcttt cgcattcgca gcaaagcatt cttcgcactc
    10081 atgctccgga aagcatggaa gtgcccagcg gaagcaagat ctccatcgac tattcgagtc
    10141 c
//
LOCUS       DAOQJL010000074        10120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3476_length_10120_cov_4.221759, whole genome shotgun sequence.
ACCESSION   DAOQJL010000074 DAOQJL010000000
VERSION     DAOQJL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_10120_cov_4.221759"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /gene="ligD"
                     /locus_tag="PLG78_05050"
     CDS             <1..2184
                     /gene="ligD"
                     /locus_tag="PLG78_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNE22508.1"
                     /translation="LSNLDKILYPGDGIVKAEVLQYYVTIAPYMLRYVRGRPLSLVRF
                     PDGIEGEQFFQKNRPDWVPEWLHSVKLGDIDYMLAEEDAAVVFLANLAALEFHQMQMR
                     PSVSQDADYMVFDLDPPENSNFEIVRDLALNLRPYLESLGYHVFVKTTGGKGLHLIMP
                     LLPHSYDIVFEAAKAAAIDFASRNKGVTLEIKKERRAQNILLDVYRNRSGQTIVSPFS
                     LRGKPGAPVSMPLSWEDLERARSPLDYNLRNAYDLIRQGDPWEGMYAHTAVPHNARAK
                     LAPVTAELETYKKKRSFDKTPEPAPASSAATGQSFVLHRHHASHLHYDLRLEQDGALT
                     SWAVPRGLPPRPGIKRLAVHTENHPIEYLTFEGKIPKGQYGAGEMWIFARGQYEITKQ
                     KKDGFYFRTHAEGLHGEYRMIHTRDKEWLMERLDEEPRLDFEPMLATQTETVPSGDMI
                     FEVKWDGIRALVFIEDGQITIRSRNGRDITAAFPEIAVPDSYHASSAVLDGEIVCLDG
                     SGRPVFQNVIRRLQNGANKKLEQKFPAFIYLFDVLSLDGKRTLKDPLHLRRAWVQDLI
                     RSGGAVRFSQSYPDGQALFDAAKEHGLEGIMAKSPDSPYVLGRRSTSWVKIKVRSTMD
                     CVIIGYTEGEGGREKLGSVHVAEKTESGLHYVGKVGSGFSDEDINDLSARLATMEIEK
                     PTVKVPKVEKGTWVEASLVCEVEYASKTRDGMLREPVFIRLRPDL"
     gene            2240..3133
                     /locus_tag="PLG78_05055"
     CDS             2240..3133
                     /locus_tag="PLG78_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNE22509.1"
                     /translation="MRSIWKGHIRFSLVTIPVRIYNAIETSESIRFNQLHRECNGRVG
                     YDKKCKKCEKSLTTEEIVKGYEYEPDQYVVFEDSDFEKVKLKSTKVIEIEGFVGVDEV
                     HPTLFESPYYAGPDGDVAARSYALLCQALKDAGKVGVGKVVLRDREDVVLLSPHEKGL
                     ALYRLRYPAEVRKMAEVPQIDKVEPADKEQLKLARNLVESMARPLSDIELKDNYRGAL
                     KEMIDAKVAGKEIVQVEEVEKPAMDIMTALKASIEKAAAMRGGMIPAKGEKKEKAQAK
                     DKAEGKEKAAKAVKTRKTKAG"
     gene            3163..3939
                     /locus_tag="PLG78_05060"
     CDS             3163..3939
                     /locus_tag="PLG78_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22510.1"
                     /translation="MANIIQFPGTRQTALQRVQSSYSLRDVQKQFGISEKLLRKWAEA
                     GAVETVPGKEGEIRFGLGALARFRSIREERTRGKTLKQIDEEMRGQLDLFASRPSGIV
                     RSLSEKRTPFEEGLILLDRGDASAASFFQKALEQDDHCADAHCNLGILAFESGDVPGA
                     MTEFIRALESEPGHFESHYDLGNLYFDAGNLPLAQMHFETAARIEPEYADTFFNLGLL
                     YARLENWKKSKECFERYTAFEDIDHGEAVKEILKHLEQQT"
     gene            complement(3972..4709)
                     /locus_tag="PLG78_05065"
     CDS             complement(3972..4709)
                     /locus_tag="PLG78_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22511.1"
                     /translation="MRLLLILLFASPLLAQENSPDFPEYLAQGTENIRDQRTGEVDIN
                     RVAQAAPTTTQPAAPVQTGLPGFAEIPWKATYSEVKTRLKNLSTAAVQTERVEILMEE
                     KNKSILVRRNEVLYRYNFYRTPVEVLRIANHDLTDADYEQQEGQLFQVRVTFSLIDAA
                     KIQDRIEKSHGAKTKSTVDPKKMEGAEIWELPGGVILEWVEPYRKMKFSRTVDFLSSE
                     MTKQIMKEYEDYFDAREKWIINNIKLY"
     gene            complement(4749..6464)
                     /locus_tag="PLG78_05070"
     CDS             complement(4749..6464)
                     /locus_tag="PLG78_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22512.1"
                     /translation="MRLSLIVTCFLTVSILHADPIAYDRISIPVEGQWKKTEKESYAM
                     FEAPGKGGIVILASAAPGLSGDVYLERLMKQHTEGRTLLSISPQKIASGKTASGVPFS
                     AQAKVTKGATDTWYVMYYVFSVEDRLQAVFVVAENAAAFKVLAKEVAPAFEKISIVAK
                     IEPAKPAEGQPATSIFQQTGPMSDQFVSGVVTAQSAELKPVPFSIQGGMRFSVMSADD
                     YDLVMSARDKDGSMYVTESSLGVFGSTDRAQFIHRIKNFGAPEKLSWSRRAVQSMIAQ
                     LSKEKIDMFFAESSSVHTLRVDEAGTLYIGANVDHDNAFIAVSKEGTSTLLASMSQLS
                     KVANGYQTGGTASRLYVSPKGTAWLMVQGIDKPGGKDYGTNAFYMRRTEEGGWRATPV
                     PVRCGGQPCSMKVKYSIFSHGVADEGGGFVFHDGNGFWRATPDGDVKNLLQLKLPGGD
                     VRIVGPVLLSNGDIWYALSTVYSVSSYSKVDQQTGYAEVRNTWFTVGDRSRFIRIRVR
                     GGKAELGEISSEAMFAALRKKGVPLRPDSQVMKTVRFVPDYATGGFIAFDAHHSVLYS
                     ILPGD"
     gene            complement(6512..7153)
                     /locus_tag="PLG78_05075"
     CDS             complement(6512..7153)
                     /locus_tag="PLG78_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22513.1"
                     /translation="MEIIVWVILFSILMGVVLVVQYWADLMRQKESYTREELLDFIRI
                     IPDRLQKLAGKDDLAMAIAALAAGIVAAWLLTLLGGLFSENVVADVPDHAEAGVPNYF
                     FQSALFVFVLHIAWPSFKDFALDRGGEEGMLFKILSAELPFFVGLSVTIASINLTCWG
                     VFHEMTFLFVLPDMLILLVYAGYRIDQVEHPHDGPGRKSGNALKRRPLENDEF"
     gene            complement(7159..7806)
                     /locus_tag="PLG78_05080"
     CDS             complement(7159..7806)
                     /locus_tag="PLG78_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22514.1"
                     /translation="MGTGEDIARAIEASIGKTAFEKLGAVSFYFVPAGRSHFRDLKRG
                     FVEVSYAAGSNQFVVQYDSLGNYVVQKNRVKISGPEAEKAHTQAVSFHINDFYWFNPF
                     VQMRSPGAILGRTESGELLVHYESGGVTPGDTYAVKVDANGRPVEWRLWVSVLPLKGI
                     GFSFDDWSEIAPGVFVSTMHKSSFKSVEIRDVKAFPSYPGQENKDRFAELAEMKK"
     gene            complement(7964..8248)
                     /locus_tag="PLG78_05085"
     CDS             complement(7964..8248)
                     /locus_tag="PLG78_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22515.1"
                     /translation="MSDPRSLSLVLFHALSGKLRRYDELTPSQARALLLESLELLPDF
                     DVHKTTVPHVVRVTTREGKSFDFNIQDFADSLVMLHRDYNRLVERMEDQA"
     gene            complement(8253..8849)
                     /locus_tag="PLG78_05090"
     CDS             complement(8253..8849)
                     /locus_tag="PLG78_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNE22516.1"
                     /translation="MRLPAVLLIALLAVSSLVSCKKSSDDSLDFATLLVLATFRDNGN
                     GTVSAPGGYTIAKCAYGQVWNAALNNCAGTGSSSTYGAQSLAYCTVANSCHDAALNAS
                     SGPAFAACSTYSVSSITGWRLPTVAELQAMTSSLSRSSLILLFTQTPDDKYFWSRETN
                     PNKSDYSEAYGISFADSTFGDKTSYNKVSVPLYVRCVK"
     gene            complement(8897..9787)
                     /gene="lipA"
                     /locus_tag="PLG78_05095"
     CDS             complement(8897..9787)
                     /gene="lipA"
                     /locus_tag="PLG78_05095"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNE22517.1"
                     /translation="MRYPETIPPAPPEWMKVRLKMGTPDSTSARAVREVLTRQRLVTV
                     CEEASCPNLGHCWSQGTATFMIMGDKCTRSCGFCDIRTAKPMPLDAAEPQRIAQAIQE
                     MKLRHAVITSVDRDDLADCGSTHFAAVIAATRALNPETTLEVLIPDFKGKRENLERIW
                     NEKPDIINHNVETVPTLYKSICPQSNYQVSLDVLRMSAQQGFLAKSGIILGLGEEISE
                     VKQVISDLKAAGTQLLTIGQYLQPSSAHAPLKEFVAPEVFDSLKDHALAAGFIHVEAG
                     PLVRSSYHAGDALDRIFGRK"
     gene            9873..>10120
                     /locus_tag="PLG78_05100"
     CDS             9873..>10120
                     /locus_tag="PLG78_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22518.1"
                     /translation="MTSQKQPFSLLRAIGLGDLAGQGFRVQFSFWSMVAMTFFLFGDQ
                     NLASPNLSRIGAAFGITDEAEYRAKIAGHASLYFFVIGG"
BASE COUNT         2651 a         2578 c         2580 g         2311 t
ORIGIN      
        1 ctgtctaacc tggacaaaat cctgtatcca ggggatggaa ttgtaaaggc ggaagtactt
       61 cagtactacg taaccattgc tccttacatg ctgcgctatg tccgtggcag acctctttct
      121 ctggtgcgtt tccctgacgg cattgaagga gaacaattct tccagaagaa caggccggac
      181 tgggtacctg aatggcttca ctctgtgaag ttaggcgaca ttgattacat gcttgcagaa
      241 gaagatgcgg ctgtagtgtt tcttgccaac ctcgcagcac ttgaatttca ccagatgcaa
      301 atgcgtcctt ccgtttctca ggatgcagac tacatggtgt ttgacctgga cccgcctgag
      361 aattccaact ttgaaattgt tcgagatctg gcgctgaacc tccgacccta cctggaatcc
      421 ctggggtatc atgtgtttgt aaaaacaaca ggaggaaagg gactgcatct catcatgcct
      481 ctccttcccc attcgtacga cattgtattt gaagcggcga aagcagcggc catagacttt
      541 gcgagtcgaa acaaaggagt gactctcgaa atcaagaaag aaagacgcgc tcaaaacata
      601 cttctagacg tgtacagaaa tcgctcggga cagaccatcg tgtctccttt tagcctccga
      661 ggaaaaccgg gagcgcccgt atccatgcca ctctcctggg aagacctgga aagagccaga
      721 agccctctgg actataactt aagaaatgca tacgatctca ttcgccaggg agatccctgg
      781 gaaggaatgt acgcgcatac agcagtgccg cataacgctc gcgcgaagct cgcgcctgtg
      841 acagcagagc tggaaacgta caagaagaag agatcctttg acaaaactcc tgagcccgca
      901 ccagcttcat ctgccgcaac aggacagtct ttcgtgctcc atcgccatca cgcatcgcat
      961 cttcactacg atcttcgact ggagcaagat ggggctctca cttcgtgggc cgtgcccaga
     1021 ggcctacctc cgcgccccgg aatcaaaaga ctggcagtgc atacggagaa tcatccgatt
     1081 gagtatctta cctttgaagg aaagatacca aagggtcagt atggcgcagg agaaatgtgg
     1141 atcttcgctc gcggtcagta cgagattaca aaacaaaaga aagatggatt ctactttcgc
     1201 actcatgcgg agggactgca cggcgaatac agaatgatcc acacgcgcga caaagaatgg
     1261 ctgatggaac gactcgacga agagccacgc ctcgattttg aaccaatgct tgccacgcaa
     1321 acggaaacag tcccgtcagg cgatatgatc tttgaagtaa agtgggatgg aattcgcgcg
     1381 ctcgtgttca ttgaagatgg acaaatcaca attagaagca gaaacggacg cgatatcaca
     1441 gctgcgtttc ctgaaattgc ggtgccggat tcctaccatg catcctccgc agttctcgac
     1501 ggagaaattg tatgcctcga cgggagcgga aggcctgttt ttcagaacgt tatacgacga
     1561 ctacagaacg gagcgaacaa gaaactggaa cagaaatttc cagccttcat ctatctgttc
     1621 gacgtattgt ctctggacgg aaaaagaact ttgaaagatc cgctccatct cagacgagca
     1681 tgggttcagg atttgattcg ctcagggggt gcagtccgat tcagccagtc ctatccagac
     1741 ggccaggcac tgtttgatgc agcaaaggaa catggcctcg aaggaatcat ggccaaatcg
     1801 cctgactctc cctacgtgct cggaagaagg tcaacatcct gggtcaagat caaggtgcga
     1861 tctaccatgg actgcgtcat cattgggtac acggaaggag aaggaggtcg ggaaaaactc
     1921 ggatccgttc atgtggcaga gaaaacggag agtggactgc attatgtcgg caaagtgggt
     1981 tccggtttta gcgatgaaga cataaacgat ctctctgcac ggctcgcaac catggaaata
     2041 gaaaagccaa ccgtgaaagt gcctaaggtc gaaaaaggca cctgggtaga agcgagtctc
     2101 gtctgcgaag tggaatacgc ttccaaaaca cgggatggaa tgctacgaga gccggtattc
     2161 attcggctcc ggccagacct gtagccccga actggtttaa acgcttgcct gtgcgattgt
     2221 gcctgacgat aatcagtgta tgcgatctat atggaaggga cacatacgtt tttcactcgt
     2281 tacgattccg gtgcgaatct acaacgcgat tgagacatcg gagagcatcc gcttcaatca
     2341 gcttcaccgc gaatgcaatg gcagagtggg ctacgacaag aaatgtaaga aatgcgagaa
     2401 gtccctgact acagaagaaa tcgtaaaagg ctacgagtat gagccagacc agtatgtagt
     2461 cttcgaagat tctgatttcg aaaaagtaaa actgaaaagt acaaaggtca ttgagataga
     2521 aggattcgtc ggtgtggacg aagttcatcc tacgctgttt gaatctccct actacgcggg
     2581 accggacgga gatgtggcag ccagaagtta tgcgcttttg tgccaggcac tcaaagacgc
     2641 aggcaaggtg ggcgtaggca aagttgtatt acgtgacaga gaagatgtgg ttcttctttc
     2701 gccacatgaa aaaggactgg cgctatacag gctccgttat ccagcagaag tacgcaaaat
     2761 ggcagaagtt ccacagatag acaaagtgga accggcggac aaagagcagt tgaagctggc
     2821 gcgcaatctg gtggaaagca tggccaggcc tctcagcgac atcgaactca aagacaacta
     2881 tcgcggcgct ctcaaagaga tgatcgatgc aaaagtagca ggaaaagaaa tcgttcaggt
     2941 ggaagaagta gaaaagccgg ccatggacat catgactgct ctgaaagcat ccattgaaaa
     3001 agcggcggcc atgcgcggtg gaatgattcc ggccaaaggc gagaaaaaag aaaaggccca
     3061 ggcaaaggac aaagcggaag gcaaagaaaa ggccgcaaag gcagtaaaaa ccaggaaaac
     3121 gaaagcgggt tgatcccata gcccggccgg cgcgaatcgg ctgtggcgaa catcatccag
     3181 tttccaggca ctcgccagac cgccctgcaa agggtgcagt cttcctatag tctgcgggat
     3241 gttcaaaagc aatttggtat ttcagaaaag cttctgcgca aatgggcgga ggccggtgcg
     3301 gtagaaaccg ttccgggcaa agaaggagag attcgctttg gcctgggtgc actggccaga
     3361 ttccgcagca tccgcgaaga aagaaccaga ggaaaaacac ttaaacaaat cgatgaggag
     3421 atgagaggcc aactcgatct gttcgcctct cgcccttctg gaattgttag gagtctctca
     3481 gagaagcgca ctccttttga agagggactc attctgctcg atcgcggaga tgcaagcgcc
     3541 gcgtccttct tccagaaggc cctggaacag gacgatcatt gcgcggacgc tcattgcaat
     3601 cttggaattc ttgcgtttga aagcggagac gtgccaggtg ccatgactga attcatacgc
     3661 gcactggaat cagagcccgg acactttgaa tctcactacg atcttggaaa cctgtacttt
     3721 gacgcaggca atctgccgct cgctcaaatg cattttgaaa ctgcagccag aatcgaaccg
     3781 gaatatgcag atactttttt caaccttggc ctgctctatg caaggcttga gaactggaag
     3841 aaatcaaaag aatgctttga acgttatacg gcatttgagg acatagatca tggagaggca
     3901 gtgaaggaaa tcctcaagca tctggagcag cagacctgat gcggtgatct tgcccgtagg
     3961 tcattcgcgg actagtaaag tttaatattg tttatgatcc acttttcgcg cgcatcaaag
     4021 tagtcctcgt attctttcat gatctgtttg gtcatttcag aggagaggaa atcaaccgtg
     4081 cgagagaatt tcatttttct ataaggctca acccactcaa ggatgacacc gcctggcaat
     4141 tcccagattt cagccccttc cattttcttt ggatctaccg tggatttggt cttcgcaccg
     4201 tgcgattttt caattctgtc ttgaattttc gcggcgtcaa tcagagagaa agtaacacgc
     4261 acctggaaga gctggccttc ttgctgttca tagtccgcgt ctgtaaggtc gtgatttgcg
     4321 atgcgcagaa cttccacagg agtgcgataa aagttataac gatagagtac ttcgttgcga
     4381 cgaaccagaa tgcttttgtt cttctcctcc atgaggattt ccacgcgctc tgtttgaaca
     4441 gcggcagtgg aaagattctt caaacgagtc ttcacttctg agtaggttgc cttccacgga
     4501 atctctgcaa atccgggcag tccagtttgg acaggtgccg ccggttgagt ggttgtagga
     4561 gctgcttgcg ctacccggtt tatgtctact tcccccgtgc gctggtctcg gatgttctct
     4621 gttccctgag ccagatactc cgggaaatca gggctatttt cctgagccag cagaggagag
     4681 gcaaacagta agatcaaaag aaggcgcata tagtatgatc ggtatgtggt aggaactcct
     4741 acatagtttc aatctcccgg caaaatgctg taaagcacgc tgtggtgggc gtcaaaggca
     4801 atgaaaccgc ccgtggcata gtccggaacg aatcgaaccg tcttcatcac ctgagaatcc
     4861 ggtcgcaaag ggaccccttt cttgcgaagg gccgcaaaca tggcctccga actgatttcg
     4921 cccagctccg ctttgccgcc gcgaactcgg attcggatga atcgggagcg atcccccacg
     4981 gtaaaccagg tattccgaac ctccgcatag cctgtctgct ggtccacttt gctgtaggaa
     5041 ctcacactgt acacagtgct cagagcatac cagatgtccc cgttggaaag aaggaccggc
     5101 cccacgatcc tcacgtctcc tccaggtagt ttgagctgta gaagattctt cacatctcca
     5161 tccggagtag cgcgccagaa accgttccca tcatgaaaca caaaccctcc cccttcatcc
     5221 gctacgccgt gagagaagat gctgtatttc actttcatgc tgcatggctg gccgccacat
     5281 ctcactggaa caggagtggc gcgccaaccg ccctcttctg ttcttcgcat ataaaaagca
     5341 ttggttccgt aatctttgcc gcctggcttg tcgattccct ggaccatgag ccaggctgtt
     5401 cctttcggag aaacgtacag gcgagaagca gtgccaccag tctggtagcc attggcgacc
     5461 ttgctcaatt gactcatact cgcaagcaaa gtggacgttc cttctttcga aacagcgatg
     5521 aacgcgttat cgtgatctac attcgctcca atgtacagag tgcccgcttc atccactcgc
     5581 agagtgtgca ctgaagaaga ctccgcgaag aacatgtcga ttttctcttt ggagagctgc
     5641 gcgatcatag actgaactgc acgtctgctc cacgagagtt tttcgggagc tccgaagttt
     5701 ttgattcgat gaatgaattg agcgcgatct gtcgagccaa acacgcccag actcgattca
     5761 gtcacataca tactgccatc tttgtctctc gcagacatca ccagatcgta gtcatccgcg
     5821 ctcataacgg agaagcgcat gccaccctga atcgaaaaag gaactggctt gagttcagct
     5881 gattgtgcag tcactacacc gcttacaaat tgatccgaca tgggcccggt ttgctggaat
     5941 atagaagtgg caggttgtcc ttctgcaggc ttcgcgggct ctatctttgc cacaatggaa
     6001 atcttctcga aagccggagc gacttccttc gcaagtactt tgaacgcagc tgcgttttca
     6061 gcaactacga acactgcctg caatcgatcc tccaccgaaa atacgtagta cataacatac
     6121 caggtgtcag tagcaccttt cgtaactttc gcctgcgctg aaaagggaac tccggatgct
     6181 gtttttccgg atgcgatctt ctgcggactg atgctcaaca gagtacgccc ctctgtatgt
     6241 tgtttcatta aacgctcgag atagacatct cccgaaagac ctggagcagc ggacgcaaga
     6301 attacaatgc ctccttttcc cggagcttcg aacatggcat acgactcttt ctcggtcttt
     6361 ttccactgac cttctaccgg aatcgaaatg cgatcatagg cgataggatc tgcgtgaaga
     6421 atggagactg tcagaaagca agtgacgatc aaagagaggc gcatgcctga tggaaccagg
     6481 cgcgcgcagc gaacgcaaca gaaaaaactg actaaaattc gtcgttttcg agcggcctgc
     6541 gtttcaacgc attcccggat ttacgtccag gtccatcatg aggatgctct acctgatcaa
     6601 tccgatagcc ggcatacacg agcaggatga gcatatctgg cagaacaaag aggaacgtca
     6661 tctcgtggaa aacgccccag caagtcaggt tgatagaggc aatggtgaca ctgaggccta
     6721 cgaaaaacgg aagctccgcg gaaagaatct taaagagcat cccctcttcc ccaccgcggt
     6781 ccagtgcgaa atctttgaaa gaaggccagg cgatatgaag tacaaataca aacagagcag
     6841 actgaaagaa atagttggga acacccgcct ctgcgtggtc cggaacatct gccaccacat
     6901 tctctgaaaa aagcccaccg agcaatgtga gaagccaggc cgcgacaatg cctgcagcaa
     6961 gcgctgcaat cgccatggcc agatcatctt tccctgccag tttctggagg cgatccggta
     7021 tgatgcgaat gaagtccaga agctcttcgc gagtgtagga ctccttctgc ctcatgaggt
     7081 ctgcccagta ctgcacgacc aggacaacgc ccatcaagat ggagaacaaa atgacccaga
     7141 caataatttc catgcgctct atttcttcat ttcggccaat tctgcgaatc gatccttatt
     7201 ttcctgtccg gggtacgaag ggaaggcctt tacgtcgcga atttccacgc tcttgaatga
     7261 actcttgtgc atggtgctta caaatacgcc aggggcgatt tcgctccaat cgtcaaaaga
     7321 aaaaccaatt cctttgagag gaaggacgct cacccacaat ctccattcca caggccgacc
     7381 gttggcatcc actttcactg catacgtatc tccgggtgta actccgccgc tttcatagtg
     7441 aaccagaagc tccccggatt ctgtgcgacc gagaatggcg ccaggagatc tcatctgaac
     7501 aaacggattg aaccagtaga aatcgttgat atgaaaagat actgcctgtg tgtgagcctt
     7561 ctctgcttct ggccctgaga tcttaacgcg attcttttgt actacgtagt ttccaaggct
     7621 gtcatactga actacaaatt gattggatcc ggcagcatac gacacttcca caaaaccgcg
     7681 cttcaaatcg cgaaaatggg aacgccccgc tggaacgaaa tagaatgaaa ccgcacccag
     7741 cttctcgaat gctgtcttgc caatggatgc ctcaatggca cgcgcaatgt cttctccagt
     7801 gcccaccgac tccggtggag acttccgcca ggaaatcagt acccaggtgg caagaatgac
     7861 cagaacactt gccagggcga agatgccttt ctttgattta atgattcgag ggattctgat
     7921 gaacttgatc tggcagttgt gccggaagac gcaataaaaa acttcaggcc tggtcttcca
     7981 ttctttctac cagccggttg tagtctctgt gaagcataac caggctgtct gcaaaatcct
     8041 ggatattgaa atcgaacgat ttcccttctc gcgttgtgac tcgaaccaca tggggaacgg
     8101 tggtcttatg cacgtcaaag tccggcagca actcaagaga ttccagaaga agagcgcgcg
     8161 cctgcgaagg tgtcaattcg tcgtagcgac gaagctttcc tgaaagcgcg tgaaaaagaa
     8221 ccagggaaag ggagcgagga tcgctcacgg catcacttca cgcagcgaac gtagaggggt
     8281 acggatacct tattgtagga agtcttatct ccaaatgtgc tgtctgcgaa gctgattccg
     8341 tatgcctctg aatagtcgct cttgttgggg ttagtctcac gagaccagaa atacttatca
     8401 tccggagtct gtgtaaatag caggatgagg ctggacctgc tcagagaact ggtcattgcc
     8461 tggagctccg ctacagttgg cagcctccag cctgtaatag aagaaacaga ataggtagag
     8521 caggctgcaa atgcaggacc agaactcgca ttgagtgccg catcatggca ggaattggct
     8581 acggtgcaat acgcaaggct ctgagcaccg taggtgctgg aagatccggt tccagcgcag
     8641 ttgttcagtg ccgcattcca gacctggccg taagcgcatt tggcgatggt gtaaccaccc
     8701 ggagcggata cagttccatt cccgttgtct ctgaaagtgg ccagaaccag caaagtggca
     8761 aaatcaaggc tgtcgtcaga ggacttcttg caggaaacaa gagaggatac agcgagaagt
     8821 gcaatcaata gaacggctgg caaacgcatg gatttcctca tcatatatat tagacgatta
     8881 gagcgagcga ttttgctcat tttcttccaa aaatccggtc cagagcatcg cccgcatgat
     8941 acgaggatcg gacaagagga cccgcttcga catgaataaa tccggcggcc agagcatgat
     9001 ctttcagaga atcaaacact tcaggcgcca caaattcttt cagaggagca tgagcactcg
     9061 acggttgcag atattgcccg atggtcagaa gctgagtgcc ggccgctttg aggtcggaaa
     9121 tgacctgctt gacttctgag atttcctccc ccaggcccag gatgatcccg cttttggcca
     9181 gaaagccttg ctgtgcgctc attctcaaaa catccaggga aacctggtaa ttcgactgcg
     9241 ggcatatgga tttgtacagt gtaggaactg tttccacgtt atgattgatg atgtccggct
     9301 tttcgttcca gattcgttcg aggttttcgc gctttccttt gaaatcagga atcaatactt
     9361 ccagggtggt ttctggattc agtgcccggg tggcggcaat gactgctgca aagtgagtgg
     9421 atccacaatc agccagatcg tcccggtcta cggaagtgat cactgcatgt ctcagcttca
     9481 tttcctggat ggcctgcgca atccgctgtg gctcagccgc atccaggggc attggttttg
     9541 cagtgcgaat gtcacagaaa ccacagctac gggtgcattt atcgcccatg atcatgaatg
     9601 tggctgttcc ctgagaccag caatgtccga ggttcggaca ggacgcctct tcgcacactg
     9661 tcaccagcct ctggcgagtg agcacttctc ggactgccct ggcgctggtt gaatccgggg
     9721 tacccatctt cagccgaacc ttcatccact ccggcggagc tggcgggatc gtttcaggat
     9781 aacgcatcag gttcagagaa acgattctgc agccccggtc gacgaaaaat tgccttgtca
     9841 gctttcttct gcccccgttt gatccccccg acatgacatc tcaaaaacag ccattcagcc
     9901 tgctacgagc cattggcctg ggcgatcttg ccggccaggg tttccgcgta caattttcat
     9961 tctggtccat ggtagccatg acattcttcc tgtttggcga ccagaatctc gcatccccga
    10021 acctctcgag gatcggagcc gcattcggaa ttacagatga ggcggaatat cgtgcgaaga
    10081 tcgcagggca tgcgtcgctc tatttctttg tcatcggcgg
//
LOCUS       DAOQJL010000075        10078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3501_length_10078_cov_4.673750, whole genome shotgun sequence.
ACCESSION   DAOQJL010000075 DAOQJL010000000
VERSION     DAOQJL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3501_length_10078_cov_4.673750"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLG78_05105"
     CDS             <1..291
                     /locus_tag="PLG78_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22519.1"
                     /translation="GELTLHNANELKELISSMVEQKKLFVLINLENVQGIDSSGLSAF
                     VAGQTYLTRQKGQLKLASARPAIRKLLKATLLDQLLAVFDSVDEAMRSFPAR"
     gene            complement(231..1151)
                     /locus_tag="PLG78_05110"
     CDS             complement(231..1151)
                     /locus_tag="PLG78_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE22520.1"
                     /translation="MKAWVKLSLTAFFWAGAFFAGKAAVTIAPPVMAAFLRFFMAGIL
                     FLILAHNQGAFREVRGTLWLNILVLAFVGIFLYNLFFFYGISLTSTTNGSLIAGANPA
                     VTALLSFVFRGERLAGRRWLGIFISFLGVVLVISQGSLEVLLHMKMNPGDLLIVCAAT
                     CWAIYSILGRDVMRQISPLAASAFACYLGSIMLFPAALATTDQVSWRSDWMFWLEVAY
                     MAIFASVLAYKWWYEGVLEIGASRSAVYVNLVPVFVMLLNVFMGHFPSEAQLGGAALV
                     LVGVYLTSGNIQRAGKERMASSTESNTASN"
     gene            complement(1174..2445)
                     /locus_tag="PLG78_05115"
     CDS             complement(1174..2445)
                     /locus_tag="PLG78_05115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE22521.1"
                     /translation="MEKKQVVIVGGGFGGINAARELSDVKGVHVTLIDKRNHHLFQPL
                     LYQVAMAALSPAEIAAPIRSVVRHSGNVDVLLGTVTGVDLNARKVKADFGEVSYDYLI
                     MACGSTHSYFGHDEWEENAPGLKSLEEALEIRRRVLTAFELAEREHDPVVQEELLTFV
                     IVGAGPTGVELAGALSEISHYTMGHDFRNIDPRRTRVYLIEGGPRVLPPFDPRLSEYA
                     QKALEKLDVIVKTNSIVTKVHEHGVVIGNEEIRARTVLWAAGVQPAEINKTLGVATDR
                     GGRVIVERDCSLSAHPEVFVLGDQAAFEAGAGKYLPGVATVAIQQGRYVASLIRQELK
                     GKERKPFQYWDKGSMATIGRALAVAQTRRIRMKGFFAWIGWLLVHIYYLIGFENRMVV
                     LFQWAYNYLTMGRGARIITNRDWHSHTKKNS"
     gene            complement(2506..3606)
                     /locus_tag="PLG78_05120"
     CDS             complement(2506..3606)
                     /locus_tag="PLG78_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22522.1"
                     /translation="MMIEQTLRLLLDGVGRSDEYEFYLNRFRTYDAPCFSFLVPDLEA
                     AVQARSSILASLDFLRKLELYPGILFAGLSSSDPRTISFGSSIQEDLLLSIEMEGPGL
                     IESRIRREAELARREKKIPVFYLRMPLEKAVHACARVTNRFQFLRVQGPIQTVSGPAP
                     YIDPHQAHEFASIQESSFSRFASGVLLQDPSLHISLCSPYRLLQEIFTVKGAGTIFRP
                     RSMVHSYTKLESVDQERLIALLESSFGRRLRDLSSLHRASHFYIEENYRGAVLLEPAE
                     PGWYLSKFAVSTLARGEGIAQDLWTAVLDTHSSVFWRAKRGNPIERWYSRIADGFQRK
                     GMWNVYWKNLSLDRIADVIRYCENRPEDFDPS"
     gene            complement(3606..4361)
                     /locus_tag="PLG78_05125"
     CDS             complement(3606..4361)
                     /locus_tag="PLG78_05125"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE22523.1"
                     /translation="MRVYGVRAALCTMFLFLIPAGLISAPAQEWMGFLDKSLAVPEGL
                     MTGRLTVSGKTGRSQVWDFNLYKKHTDLLYLFSTKRRGLELKLLYKSGGEVCWMWDLL
                     RASLFRKRDLEKFEPVLGSGFSFMDLSGALYESQYEPVDLREASTMVLKIKPVLPSGY
                     TEILAQMDREKKRPLRIDFHGKDKILIKTMRFTYDLPLLDPRARKRISMAAPAVQDVI
                     DLETGRISRIEFFSLDRAVSPDDSLFIPEFLNR"
     gene            4426..5409
                     /locus_tag="PLG78_05130"
     CDS             4426..5409
                     /locus_tag="PLG78_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22524.1"
                     /translation="MIRIIATIFCALLAMPAFADKVRVNLGFWPGELQPEQENRLLKD
                     DLIAGASVGRLDLEWTNKETYTIYPLGVQYMMDGIGPGTLIIGGNYIRYSPAYKFNAL
                     TLGPSIGIINLQNFKNSDWEAEAGYQLALLDKKLLVTPKFGARWHFQEYDYDELTIGV
                     GSYAKSFDSPFRANAKGTYVGSGVQYYVTPELSILGEYIMSTPFFGNVSGAMTDKRNT
                     VGVVASVPYIQIERANAGYEVEIKRWMLGVQYDVMPQLHIQAGLRQEIQTAKYTGYFN
                     LPILITGTSVATISPTLNTVGEIITDKLFWEAAETQKKGFVFIGASYDVNL"
     gene            5442..6689
                     /locus_tag="PLG78_05135"
     CDS             5442..6689
                     /locus_tag="PLG78_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNE22525.1"
                     /translation="MPFYIFSTGTELSSGRSRDTNGPFIAQSLSEAGLDVSGIITLPD
                     NPTSILEELSRLIARTDTSGIIITGGLGPTEDDHTVDILCKLTEKSAVEDPFALSRLE
                     AAAKRLPGRLQLSSARRQTRVVEGCTVIKNERGLAPGMIVEIGTNGPFIAAMPGVPSE
                     MRGMFDGTLFPYIKSRFVSHSRERLSFFIYGHGESQVQARLFGDKETQRSIPETLPDD
                     FIWGITAQPGFSKVFFETTDKSFLGLIDQAAENIFGNAKLDATVEDKLHDYCIENKIK
                     VALAESCTGGLISRILTDRPGASAYFQGGAVVYANAAKEQILGVSSAILETHGAVSEE
                     CAMAMAEGARRIYSADASVAITGIAGPEGGTAAKPVGTVHIAASYKDKSMHAKLFLPF
                     DRDRIREYSARVAILHLFNLLRS"
     gene            complement(6702..7676)
                     /locus_tag="PLG78_05140"
     CDS             complement(6702..7676)
                     /locus_tag="PLG78_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22526.1"
                     /translation="MAPRHRVFIAFCFTFAAFCIAAWFLLPESGSWLETDEFVWRAKT
                     DHLARGFSAHTVILGDSQAMSGILPPAGVYNLALPSQQPEGVERMARDVGQVRGLRRV
                     IINVSPFFLFKSEVLQSFLSYYRNTYDWSFADLNPDLMTLSGKNTGDSVYRMIRFLPV
                     LRLRDRMSPALASADPISFVAGRKETNLKIEKILNDQRGFWVWKSTDQTCNADIPPPP
                     PMLLAYADRPGAVESLERSVQGLRARGLEVFLVYIPLSDVWASLALRGTDDRVIAVMR
                     RMEKVGSVTLPIADRAQYKGLFRDWTHLNRCGAEKFTSWLFSQSLLQN"
     gene            7755..8774
                     /locus_tag="PLG78_05145"
     CDS             7755..8774
                     /locus_tag="PLG78_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22527.1"
                     /translation="MQPAGTRFLLTTSLFFLACGPSRKDGGLPLHVVIDAGSSGTRIC
                     LYGIRTEGNECHAVDTDFACKDVPAENGLADIGPERAEQTVNAALQMVEPQSSRIKGA
                     VLLGTGGFRRATQDRQTTVMRAAGSALLTKGYPAVLKVIPGEEEGRLAWYSIAETTGS
                     KAHATLEIGGATVQMAVGIGKNVRAVSTSDGMNDSLKKLGDASACLRPGTKESHEVCV
                     TEIRDRIFLNSRLSDFAMNLSREEKVLPLYGLGAPWNAVFKRSGKQELTLKEIEQNAQ
                     EICSKSVEELARSIPLEYAKRSCYLHSYASELLRITGQTRIKRGSESWPRGASIAKEY
                     FSDCR"
     gene            complement(8799..9404)
                     /locus_tag="PLG78_05150"
     CDS             complement(8799..9404)
                     /locus_tag="PLG78_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE22528.1"
                     /translation="MRNKRSQDSASAVLAAFKMPRGRKVTQRISSVTAAFVQAIVPVI
                     NPTATQISGALRVLGIKASKLTCAYCGRTADQWDHLYALVMNKKPTGYITEINNLVPS
                     CGACNQRKRGTNWKDFVNHPGKNFPKTFRDGFRKRLAALEKYARLRRQKIDFEKWEGD
                     PAYSDYWKRHRAVIKALRAAQTTATHLRTKLLDTVPSKRRQ"
     gene            complement(9495..>10078)
                     /locus_tag="PLG78_05155"
     CDS             complement(9495..>10078)
                     /locus_tag="PLG78_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22529.1"
                     /translation="ILPTSFASTGGIPAEVRKFYRGKKEETAYNIRFSPIENPKVADP
                     YLRFNLLLQLDDRSSCTVTPIRFYKLCGEVSGADVQTDELACVDLDVQKVLQDPLLVR
                     FVQEAGQDCVAANKRQAEQALRARQDQAYQQATDRERRTRCAQIAYNLPGGMVPSSRG
                     QIPHVCAKGWRYGSADWVKCRDELESCWDVLSQ"
BASE COUNT         2650 a         2715 c         2443 g         2270 t
ORIGIN      
        1 ggcgaattga ccctgcacaa tgcgaatgag ctaaaggagc tgatcagtag catggtcgaa
       61 caaaagaagc tattcgtatt gattaacctg gaaaatgtcc aggggattga ttcttcagga
      121 ctcagcgctt tcgttgccgg acagacgtat cttacgcgcc agaaaggcca gctcaaactg
      181 gcctcagcca ggccagcgat tcgcaagcta ctgaaggcca cgctactcga tcaattgctc
      241 gcagtgtttg attctgtgga cgaagccatg cgttcttttc ccgcgcgctg aatattcccg
      301 ctggtcaaat acaccccaac caggaccaga gccgcgccgc cgagctgagc ttcgctcggg
      361 aaatgcccca taaaaacatt cagaagcatc acaaagacag gcaccagatt tacatagact
      421 gcagaacgcg aagcccctat ctcgagtaca ccttcatacc accacttata agcaagaacg
      481 gaagcaaaga tcgccatgta ggccacttcc agccagaaca tccaatcgct gcgccagctc
      541 acttgatctg ttgtggcaag cgccgccggg aaaagcatga tggatccaag atagcaagca
      601 aaggcggacg cagcaagtgg cgagatttgc cgcataacgt cccgaccaag aattgaatag
      661 atggcccagc atgtagcggc acaaacaatc agcaaatcac ctggattcat tttcatgtga
      721 agaagaactt caagcgatcc ctgcgaaatg accagaacaa ctcccaaaaa ggaaatgaaa
      781 ataccaagcc accttctgcc tgctaaacgc tcacctcgaa atacaaaact gagaagagca
      841 gtcaccgctg gatttgcacc ggcgatcaag gatccattcg ttgtagatgt aagactgatt
      901 ccataaaaga aaaacagatt gtagagaaag attccaacaa aggcgagaac gagaatgttc
      961 agccacagag ttcctctcac ttcgcgaaac gctccttgat tgtgcgcaag gatgaggaag
     1021 aggattccgg ccataaagaa tcgtaagaat gcagccatca caggaggcgc gattgtgacc
     1081 gccgcctttc ctgcgaagaa agcccctgcc cagaagaaag ctgtgagact gagctttacc
     1141 caggctttca ttcagattcc ggtatgggtc tgatcacgag ttcttctttg tatgactgtg
     1201 ccagtctcga ttcgtgatga tgcgcgcccc tcgacccatg gtcaaatagt tgtacgccca
     1261 ctgaaacaga accaccatcc tgttttcaaa tccaatgaga tagtaaatgt gcacgagaag
     1321 ccagccaatc catgcaaaga atcccttcat gcgaatgcgt ctggtttgtg ctaccgcaag
     1381 agcacgaccg atcgtagcca tgctgccttt atcccagtat tgaaatggtt tacgttcttt
     1441 accttttagc tcttgtctga tcaagctggc aacatagcgc ccttgctgaa tcgcaaccgt
     1501 ggccacacct ggcagatact tacccgcccc ggcttcaaac gctgcctgat ctccgaggac
     1561 gaatacttcg ggatgtgcgg atagactgca atctctctct acaatgacgc gaccgcctct
     1621 gtccgttgcc acacccaggg tcttattgat ttcagcgggc tgtacaccgg ccgcccagag
     1681 aaccgttcga gcacgaatct cctcgtttcc aatgactact ccgtgctcat gaacctttgt
     1741 tacgatgctg tttgtcttta cgatcacatc gagcttctca agcgcctttt gtgcatattc
     1801 agaaagcctg ggatcgaagg gaggcagtac tcgaggacca ccttcgatga gatacaccct
     1861 ggttcttcgc gggtcaatgt tgcgaaagtc atgacccatg gtgtagtgac tgatttcaga
     1921 tagagcgccg gccagttcca cgccagttgg accagcacct acgatcacga aagtgagcag
     1981 ctcttcttga accaccggat cgtgttcgcg ttctgcgagt tcaaaagcag tgagcactct
     2041 tcttcgaatc tcaagcgctt cttccaggga ctttaagccc ggggcatttt cttcccactc
     2101 gtcgtgaccg aaatagctat gagtcgatcc gcatgccatg atcagataat catacgaaac
     2161 ttccccaaaa tccgctttta ccttccgcgc attcaaatcc acacctgtga ccgtgccaag
     2221 aagcacatcc acattccccg aatggcgcac aacagaacga atgggagctg caatctcagc
     2281 cgggctcaga gcggccatgg cgacttgata aagaagcggt tggaagagat gatggtttcg
     2341 cttgtcgatc agagttacgt gaactccctt aacgtccgac agttcacgcg cagcgttgat
     2401 cccaccgaat cctccgccta cgattacaac ctgtttcttt tccatgcgtt cgctccctgg
     2461 cagattcaaa aaaacaaagg ggccctgcga gtcaaagtag aaaaatcagg acggatcaaa
     2521 atcctcagga cgattttcgc aatatcgaat aacgtcagca atgcgatcca ggctcaggtt
     2581 tttccagtaa acattccaca tccctttgcg ctgaaaacca tccgcaatac ggctgtacca
     2641 tctctcgatt ggattgcctc tcttcgctcg ccagaatacg cttgaatgcg tgtcgagcac
     2701 tgcagtccac aggtcctgcg cgattccttc tccacgcgcg agagtactga ctgcaaattt
     2761 cgaaagatac cagcccggct cggctggttc cagcagaacc gcaccgcgat agttctcttc
     2821 tatgtaaaaa tgcgaagcgc gatgaagaga agacaggtcg cgcaatcgtc taccgaagct
     2881 tgactcgaga agcgcaatca aacgctcctg gtccacgctc tccagttttg tgtagctgtg
     2941 aaccatcgaa cgtggcctga aaatggttcc ggctcccttc acagtaaata tttcctgtaa
     3001 cagcctgtaa ggtgaacaaa gcgaaatgtg aagcgatgga tcctgaagaa gaacgccaga
     3061 agcgaaacgc gaaaaagaag attcctgaat cgatgcgaac tcgtgcgcct gatgaggatc
     3121 aatgtatgga gcaggaccgg acacggtctg aatggggccc tgcactctca ggaattgaaa
     3181 gcgattggtg actcgtgcac aggcatgcac tgctttttcc aggggcatcc gcaaatagaa
     3241 aacagggatc ttcttttcgc ggcgtgcaag ctctgcctct ctgcgaatcc ttgattcgat
     3301 gagtccggga ccttccattt caatcgaaag aagaaggtcc tcctgaatcg aggatccaaa
     3361 agaaatagtg cgagggtcag aactcgacag acccgcgaac aggatgccag gataaagttc
     3421 gagctttctt aaaaaatcaa gactcgcaag aatagaagag cgcgcctgaa ctgccgcctc
     3481 aagatcaggc accagaaaag aaaagcacgg cgcgtcatac gtgcgaaagc gattgagata
     3541 gaattcatat tcatcgcttc tgcccacacc atcgagaagc aatcgtaacg tttgttcgat
     3601 catcatcagc gattcaaaaa ctccggtatg aaaagtgaat cgtccgggga aacagcgcgg
     3661 tccagactga aaaattcaat tctagaaatt ctgcctgttt ccagatcgat tacgtcctgc
     3721 actgcaggag cggccatgga aattcttttt cgagcacgtg gatccagaag cggcaagtcg
     3781 tacgtgaatc tcatggtctt gatgaggatc ttgtctttgc cgtgaaaatc aattcgtagc
     3841 gggcgctttt tctctctatc catctgagct agaatttcag tgtacccgga cggaagcaca
     3901 ggtttgatct tgagaaccat agtcgacgct tcgcgcaggt ccaccggttc atactgagat
     3961 tcgtacagtg ctccagacaa atccataaaa gaaaatccac ttcccagcac tggttcaaat
     4021 ttctccagat ctctttttct aaagagagaa gctcgcaaca agtcccacat ccagcatact
     4081 tctccgccgc ttttataaag aagctttagc tcaagaccgc gcctctttgt ggaaaacaga
     4141 tagagtagat ccgtgtgttt cttatataga ttgaagtccc agacctgaga gcgcccggtc
     4201 ttacccgaca cggtcagtcg cccggtcatc agaccttccg gaaccgccag ggatttgtcg
     4261 aggaagccca tccattcctg agcaggagcg gaaataagcc ccgcaggaat cagaaacaaa
     4321 aacattgtac aaagagcggc ccgcactccg taaactctca cggtaccctt tccggtgtcg
     4381 atcgaaaagg ttgaccctat gtctgattat gtctttcatg gcggcatgat aagaatcatc
     4441 gcaaccatct tctgcgcgct cctcgccatg cctgccttcg cagacaaagt gcgcgtcaac
     4501 cttggcttct ggccaggaga gctacagcca gaacaggaga atcgtctcct caaagatgac
     4561 ctcatcgcag gcgcaagcgt aggccgcctc gatctagaat ggaccaacaa agaaacctac
     4621 accatatatc cactgggcgt tcagtacatg atggatggaa ttggacctgg aacattgatc
     4681 atcggtggca actacatccg atattcgcct gcgtataaat tcaacgctct cacgctcggg
     4741 ccaagcattg ggatcatcaa ccttcagaat ttcaaaaact cagactggga agcggaagcc
     4801 ggataccagc tcgcacttct agacaaaaag cttcttgtga ctcccaagtt tggagctcgc
     4861 tggcactttc aagagtatga ctacgatgag cttaccattg gagtgggttc gtacgcaaaa
     4921 agctttgata gcccatttcg cgcaaatgcg aaaggaacct acgtaggttc aggtgttcag
     4981 tactacgtaa cacctgaact ttccattctg ggtgagtaca tcatgagcac acctttcttt
     5041 ggaaatgttt caggcgcgat gacagacaag agaaacacag tcggtgtagt ggcctccgtt
     5101 ccatacattc agattgaaag agccaatgca ggctacgaag tggaaatcaa acgatggatg
     5161 cttggcgttc agtatgatgt aatgcctcag ctccacattc aggctggcct tcgccaggaa
     5221 atccagactg cgaaatacac tggatatttc aaccttccca tcctgatcac cgggacatca
     5281 gttgctacca tttcgcctac gctcaacaca gtcggagaaa tcatcacaga caaactgttc
     5341 tgggaagcag cggaaacaca aaagaaagga ttcgtcttca ttggcgcttc ctacgatgta
     5401 aatctctaaa aaacactgcg ggcctccggg cccgctcttc catgcctttc tatatttttt
     5461 ccaccggcac tgaactttca agcggtcgat cgcgcgatac gaatgggccg ttcattgcgc
     5521 agagtctgag cgaagcagga ctcgatgttt ccggcatcat cactctacca gacaatccga
     5581 cctccatcct ggaagagctt tcgcgcctga tcgcgcgcac cgacacgagc ggaatcatca
     5641 tcactggagg ccttggacca acggaagatg atcacactgt agacatactc tgcaagctca
     5701 ctgaaaaatc agccgtagaa gacccgtttg ctctttccag actcgaagcc gcggccaaac
     5761 gccttcctgg gcgactgcaa ctttcttccg cgcgaagaca aacacgagtt gtggaaggat
     5821 gcactgtaat caagaacgaa aggggactcg ctcccggaat gatcgtagaa ataggaacaa
     5881 acggaccatt cattgcggct atgccaggag tcccttccga aatgcgcggc atgttcgatg
     5941 gaactctctt tccttatatc aagtccagat tcgtttctca cagtcgcgaa aggttgagtt
     6001 tctttatcta cggtcacgga gaatcccagg tgcaggcgcg tctctttgga gacaaagaga
     6061 cacagaggtc cataccagaa acgcttcccg acgatttcat ctggggcatc acggcgcaac
     6121 ccggtttctc gaaagtattt ttcgaaacca cagacaaatc gtttctgggt ttgatcgatc
     6181 aagcagcaga gaatattttc ggaaatgcga aactcgatgc aaccgttgaa gacaagttgc
     6241 acgattactg catcgaaaac aaaatcaagg tggcgcttgc agagtcctgc actggcggct
     6301 tgatttccag gatcctcaca gacagaccag gagcatcggc ttattttcag ggcggagcag
     6361 tcgtatacgc aaatgcggcc aaagagcaaa ttctaggtgt atcatccgcc attttagaaa
     6421 cgcacggagc tgtaagcgaa gaatgcgcca tggcgatggc agagggggca aggagaatct
     6481 attcggcaga cgcgtccgtt gcgatcacag gaatcgcggg cccggaaggt ggaactgccg
     6541 caaagccggt tggcacagta cacattgccg cctcctacaa agataagtcc atgcatgcga
     6601 aactgttttt gccctttgac cgggaccgca tccgagagta ttcggcgcga gttgccatct
     6661 tacacctttt caatttgctc aggtcctgag cgcttccaga atcaattctg caacagtgat
     6721 tgcgaaaaaa gccaggaagt aaatttttct gcaccacaac gattcaaatg agtccagtct
     6781 ctaaacaagc ctttgtattg cgctctatct gcgatgggca aggtaacaga acccaccttc
     6841 tccatccttc gcataacagc gatcacacga tcatctgttc cgcgaagagc gagagatgcc
     6901 cagacgtctg aaagaggaat gtaaaccaga aacacttcaa ggccgcgagc gcgaaggcct
     6961 tgaacgctgc gctccaatga ctctactgcg cctggtctat ccgcataggc aagaagcatg
     7021 ggcgggggtg gaggtatatc tgcattgcat gtctggtctg tggacttcca gacccagaac
     7081 cctctttggt cattcagtat cttttctatc ttgaggtttg tctctttcct tccagcaaca
     7141 aacgaaatcg gatctgccga cgcaagagcc ggagacattc gatcgcgcaa acgaagaaca
     7201 ggcaaaaaac gaatcatcct gtacactgaa tcgcctgtgt tcttcccgga cagtgtcatc
     7261 aaatcaggat tcaagtccgc aaaagaccaa tcgtatgtat ttctatagta actcagaaag
     7321 gactgcagca cttcgctttt gaaaagaaaa aaaggactaa cattgatgat cactctacgg
     7381 agccctcgca cctgccccac gtcccgcgcc attctctcca ctccctcagg ctgctgagaa
     7441 gggagtgcga gattgtagac accggcgggc ggcaggattc cgctcatcgc ctgactgtcc
     7501 ccgaggatga ctgtatgagc ggagaatccc cgggcaaggt ggtctgtctt tgcgcgccag
     7561 acaaattcgt ctgtctccaa ccaggacccg gattccggga gaagaaacca ggcagcaatg
     7621 cagaacgccg cgaacgtgaa gcaaaatgcg atgaacactc tgtgccgcgg ggccatgatc
     7681 catgcacgat caccctttgt ccggcgcatc cagaaaaatg ttgtgcgcgc gcacggcggc
     7741 cgttctgtca gacaatgcaa ccagccggca cccgattcct tttgactacg tcactgtttt
     7801 ttctggcctg tggtccttcc cgaaaagacg gaggactgcc ccttcatgtg gtgatcgacg
     7861 ccggctcttc cggaacgcgt atttgcctgt acggaattcg cacagaggga aacgaatgcc
     7921 atgcagtgga taccgatttc gcatgcaaag atgtgccggc ggaaaacggc ctggctgaca
     7981 tcggaccaga gcgcgccgaa cagactgtga atgccgctct tcaaatggtg gaacctcaat
     8041 cgagcagaat caaaggtgcg gtgcttcttg gcacaggagg attcagaagg gcgactcagg
     8101 atcgacaaac cactgttatg cgagcggcgg ggagtgcact tttgacaaaa ggataccctg
     8161 cagtactcaa agtgatcccc ggggaagaag aaggcaggct cgcctggtat tccatagcgg
     8221 aaacaaccgg atcgaaagcc cacgcaactc tcgaaattgg cggagcaacc gtgcagatgg
     8281 cagtgggaat cggcaaaaat gttcgcgccg tttcaacatc cgacggcatg aacgattctt
     8341 tgaagaaact gggagatgca tccgcctgtc tgcggcccgg tacgaaggaa agccatgaag
     8401 tatgcgtaac tgaaatcagg gacaggatct ttctgaactc ccggctttct gactttgcga
     8461 tgaatttgtc cagagaggaa aaagtccttc ctctctatgg actcggcgcc ccgtggaacg
     8521 cagtattcaa acgttcaggc aagcaggaac tcactctgaa agaaatcgag cagaacgccc
     8581 aggagatatg ttcgaaaagt gtggaagagc tggcacgctc catccctctc gagtatgcta
     8641 agagatcgtg ctatctgcac tcttacgcct ccgaacttct tcgcataaca gggcaaacac
     8701 gaatcaaacg cggatcagaa tcctggccgc gcggcgcatc catcgccaag gaatattttt
     8761 cagactgcag gtagtgcaaa ccgccgggct gcggcccgct attgacgtct tttggatggg
     8821 accgtgtcga gaagcttggt tcgaagatgg gtggcggtcg tttgagctgc ccgcagtgcc
     8881 ttgatcacgg ccctatgccg tttccaataa tcactgtacg ctgggtcgcc ctcccacttt
     8941 tcgaagtcta ttttttgacg gcgaagccgg gcatactttt ccagcgctgc tagacgcttt
     9001 cgaaagccat cccgaaaggt ctttggaaaa ttcttacccg gatgattcac gaaatctttc
     9061 caattggtac cgcgcttccg ctggttgcat gcaccgcaac ttggcacaag attattgatc
     9121 tccgtaatgt aacctgtcgg cttcttattc attacaagcg catacaaatg gtcccactga
     9181 tcggcggtcc gtccgcaata cgcacaggta agtttgctgg ctttgattcc tagcacccgt
     9241 agcgccccgc taatttgagt ggctgtcgga ttgatcaccg gcacaatggc ctgcacgaac
     9301 gccgcggtca ccgaggatat tcgttgcgtg actttacgcc cgcgcggcat tttaaatgca
     9361 gcaagcacgg cggacgccga atcttgtgac cgcttattgc gcataattac aatctcccaa
     9421 gccagtaagc cacgaccttg cgcttcgatt ctgtacctct agtagatcga cgcggccaga
     9481 ttttcgtgat catttcactg cgaaagcacg tcccagcaac tttccagttc atcgcggcat
     9541 ttcacccaat ccgccgaccc ataacgccag ccctttgcgc aaacgtgcgg aatttggccc
     9601 ctactgctgg gtaccatgcc cccgggcaaa ttatatgcaa tttgggcaca ccgggtccgg
     9661 cgctctctat ccgtagcctg ctgataagct tgatcctgcc gcgcgcggag ggcctgctcg
     9721 gcttgccttt tattggcagc aacgcaatct tggccagctt cttggacaaa ccgcaccaat
     9781 agcggatctt gcagaacttt ctgcacgtca agatcgacgc aagccaactc gtccgtttgt
     9841 acgtctgcgc cgcttacttc tccgcacaat ttgtaaaatc tgatcggtgt tacggtgcag
     9901 gagctacgat catcaagctg caggagcagg ttgaatcgaa ggtacggatc tgcaaccttc
     9961 ggattttcaa tggggctaaa cctaatatta taagccgtct cctctttttt gccgcgatag
    10021 aatttcctaa cttcggccgg aatacctcct gtggaagcaa acgatgtcgg taagatga
//
LOCUS       DAOQJL010000076        10049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3516_length_10049_cov_4.333400, whole genome shotgun sequence.
ACCESSION   DAOQJL010000076 DAOQJL010000000
VERSION     DAOQJL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3516_length_10049_cov_4.333400"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PLG78_05160"
     CDS             complement(<1..1328)
                     /locus_tag="PLG78_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNE22530.1"
                     /translation="MIRIKVALLLLLVCLSSLASQPQSQAAYDRGIDALYNQKQPDRA
                     LEFFREAYRLDPNVWSRPFMIGYTLNAYLNQPSEALPFLNRAWALNDANDELPYKETI
                     QCLEKLQRWDAAIAKNQDAQKRLKAARKTIGSWYPENLAWLYWQKGDTTRALAIAPAG
                     SWVRSQLAPRTIEFTWRLQLAKLLEEWRLTSFDKIRLSLPIDRPFQSLKSVSLTSSLG
                     DSLKTRRVARRGNNFLELEKAQEDEWPAEVVLKVKVEQNLKNMVARPAGLQPARVGDE
                     NYEWAVENKDGLFSQDDPEFIAKVEEISRPGRTTGEKADLLLNYLRANFKYGKRVEGD
                     TVHSWLKQGTGDCGYFTYIAIGMLRAIKVPVRGLYGIGPWTDPPPALPHSILEIYDAS
                     TGQWFPHDPQVASQYGIIDPSYVPLMAANPKQDVAVLSPDNVWELDTTWF"
     gene            1556..3712
                     /locus_tag="PLG78_05165"
     CDS             1556..3712
                     /locus_tag="PLG78_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE22531.1"
                     /translation="MASAVYNGDAVRAKQLLDEGVPPNHIMPENLGHSFLTFACLQGH
                     LPVVQILIEAGAHINRKDSFRTSSGTDAGYYPLMAGCNARDHAPEIAKYLLEHGAKVD
                     LAHPSGRDGAIHFAAMRGDSAVVKLLLDEGVNVNQPGSYGSTPLTLALHMNSTSNLVR
                     MLLNAGAEIDSSPNKPNPLFELIAGWLDEADLARLFIDHGVPTTDASGSAIVQAASRG
                     KTAILKTLLENKPLSSPDTTDAVLAAMKGQHFDAVEFLLRHGADPNAAERFEGTLLRQ
                     ATRAGNTKLVSLIRLKQKEKSETRNTGAMLAAAENGDLAMMRLLFESGLPIDEVHDGE
                     VWGSKKETPLMRAAARGRMEAVEYLMLHGASVDAVDEKGNTPLMYAAAAGQTEAIGYL
                     LDHGASIDSTNHLNWNALMQACFTGKESAARLLLERGSRTDPIDLEKGATALSLARMS
                     ENTNLVKMLEERGVTDRLRRRRASNQQYAGIADCDICTYMAPRQELTHSYTPEKTEDL
                     LIIHEEGSVDYKQEITRMIKKCKECGTYYYHYHFIDTEDSIGGAGPICSHYLIRLNLL
                     WALDLFSKLEKTGELGELNQRYSQLIESFAILLRAENHIIPPYVLPTVVESLNDFYVM
                     KSSWNSIVTLLCHPTIAVLTLEDILRVFGENARDDTYPPFTPYRNFTKDAHDKMRKLL
                     RKNQKEFREHLNRIARSCDQKTSDFFLRTAQAYKIG"
     gene            3715..4638
                     /locus_tag="PLG78_05170"
     CDS             3715..4638
                     /locus_tag="PLG78_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22532.1"
                     /translation="MLDEYCDTLSDPALLEIALRLTELSLPVWDKHFSSHPEEERKLA
                     RAMNEATRVPGARTLDAHFPETALSMIEKCYADSKTGPSALEKLRGNPELIHLLCTSM
                     QPLTNQEWDEAFPRSVRLVFTSVWNILSWLVYTQYSSSDENHIYVAINQAADALLSEG
                     LFSVEQINAILAEYKNRIYPNEINLYPSRTDRSKNSSAMQGFAENDAYTFIATGERAP
                     GNAPADSQRRLILRQMKEENKSIWDSDYEYFNGISRTYSYNHEQESYWITEIDIIAAS
                     FCNERALTEEEMLAALSDFSLHHLRESGFTF"
     gene            complement(4644..5390)
                     /locus_tag="PLG78_05175"
     CDS             complement(4644..5390)
                     /locus_tag="PLG78_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22533.1"
                     /translation="MKFASAICILFFASLSGLIAKPGDYKELGGGWDGTRAMTALDGA
                     IYAVHGGKLYRTDSSGKYTELGDGGWSETSAMCGMDGALWIIDGGKLYRVDKNGKYAE
                     VASGWSKTRLMTTLDGSLWIIDNGTLYKVDKNGKYVEVGEGGWSKSSAMVAFDGALWI
                     IDSGKLYRTDKTGKYTELGDGGWSSTSAFVAAGGSLWAVDGNKLYKIDRAGKYTEIGG
                     GGWTEVEGAASIGDDLFIISGSKLYRASTK"
     gene            5541..6149
                     /gene="thrH"
                     /locus_tag="PLG78_05180"
     CDS             5541..6149
                     /gene="thrH"
                     /locus_tag="PLG78_05180"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNE22534.1"
                     /translation="MRPTIFVMDLEGCLIPEIWLHIADKTGIADLRKTTRDVADYREL
                     MNGRLAVLDRHKIKLSDIQAMLADLGILEGARDFVEWVREKTEFIILSDTYVEFAKPL
                     MKGLGNPTLFCHRLTTNAAGQIDGYALRSEDSKADIVRALKGNGFRIMAAGDSFNDSN
                     MLIQADNARWFRAPASIVEKFPQFPALTEYQDLKNAISQFLL"
     gene            complement(6118..6954)
                     /gene="cobA"
                     /locus_tag="PLG78_05185"
     CDS             complement(6118..6954)
                     /gene="cobA"
                     /locus_tag="PLG78_05185"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNE22535.1"
                     /translation="MSAWCDPLEKFTLVGLEEGQHPKGKVYLVGAGPGDPDLLTIKAS
                     KIIQSADVILYDSLIGPEILKFASPNARRIFVGKRAHSHSYTQDQINEMILEEASKHH
                     TVVRLKGGDPFIFGRGGEEIQFLREHGIELEVIPGVTAATAASALLQSPLTHRDLGAS
                     VVFLSGYSRADRESDDGFPHHDWKYLASSSLTLVFYMGQHHLSRICQSLIEAGREENT
                     SIALVSNISLPEESVTLTTISHARDPEVSRSVRFPAVAIVGDVLRMMDPALKARIAKS
                     HS"
     gene            7018..8784
                     /gene="cobT"
                     /locus_tag="PLG78_05190"
     CDS             7018..8784
                     /gene="cobT"
                     /locus_tag="PLG78_05190"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNE22536.1"
                     /translation="MGENRTPFSEEQIKGVYRAIHERRDIRAYRPEAVEDGVLQRLLE
                     AAHHAPSVGFMQPWNFILITDKARRRALYEHFLQCNEEASQHFEGERKLKYDSLKLQG
                     ILDAPINIVFTCDRSRGGKHVLGRNTIPDTDIYSTCLAIENFWLAARAEGLGVGWMSI
                     HKIETLHSILEIPAHVLPVAYLTLGYPVSFPDAPLLESTGWRAREDLTNLVYENVWGQ
                     LKEFAPKEQPPKAGGHIQTSTESSAGRSIGREAETVRHRLDHLTKPRGSLGDLEDWIV
                     HLAAIQKRNVPLCDHPHILLFAGDHGIAAHHISAYGQHVTAQMMYQYVAGTAAINSIA
                     RANGIALSIYDVGVNHDFGNAEGIQHRKVKMGTRDFQIDCAMTPDECRAAYEHGRSSV
                     LELPDTCSIIGLGEMGIGNTTAASAIACALLGDSAENLTGAGTGIGPDTRKRKIEIIE
                     KGLSLHRQQIASPMDAIARLGGYEIASLAGAIEGAFEKNIPVVLDGFITGVAALAAVR
                     HRPEVHSVLIAGHSSKEKAHAQILAKLNLRPILNLNLRLGEGSGAALAMGLIANACRL
                     YSDMKTFEEANIIFAENEQARL"
     gene            8801..9289
                     /locus_tag="PLG78_05195"
     CDS             8801..9289
                     /locus_tag="PLG78_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22537.1"
                     /translation="MKSLSTPIQFVVAVLCAACVTSLQAQSGLRIEASYASIEHSMDS
                     NSTHETFTLAGSKLTYNYEYGGYHPGPNFERKRTDSKKVNPEDLHKFVMEKKLNRSLN
                     RKVVSSSPHAREVHLHLDIRMGAETFVLNFDGTRPDAGADKDFDNALAFCDLMRSIVS
                     NP"
     gene            9336..>10049
                     /locus_tag="PLG78_05200"
     CDS             9336..>10049
                     /locus_tag="PLG78_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22538.1"
                     /translation="MRILIVGAGSVSGKSALQAAKAAGATVIATTSRKENLPGADHTI
                     HGIDLENPAAVDLILADRFLTQQPLDYIAYIPARGAVGISLEEAEASMVEPSLSYSVR
                     PYLKLHKALHPKKTIALSGFITMEPLLKIYGAMCFTKIAMEQLAVDHPKDLQIIRIGM
                     FHSNSVRGIMILAQRRFMRDPSYQPEWRAEWKASGKKFPEFFYAKNYKCEEETYRIHG
                     QGKPFRPTEPSDIAEGFRLA"
BASE COUNT         2683 a         2661 c         2449 g         2256 t
ORIGIN      
        1 aaaaaccagg ttgtatccag ctcccaaaca ttgtcaggac taagaaccgc cacatcctgc
       61 ttaggatttg cggccatcag tggaacgtaa gaaggatcaa tgataccgta ctgcgaagca
      121 acttgaggat catgcggaaa ccactgtcct gtgctcgcat catagatttc gagaatggaa
      181 tgaggaagcg cgggcggcgg atccgtccat ggaccgattc cgtacagtcc tctgaccgga
      241 accttgatgg cacgaagcat accgatcgca atgtaggtaa agtagccgca atctcctgtg
      301 ccctgcttta gccagctgtg cactgtatct ccttccactc gctttccgta tttgaaattt
      361 gcgcgaagat aattcaaaag tagatccgct ttctctccag tggttctgcc cggacgtgat
      421 atctcttcca ctttcgcgat gaattccgga tcgtcctgag aaaagagtcc gtccttgttt
      481 tccacggccc attcatagtt ctcatctccc acacgggcag gctggaggcc ggcagggcga
      541 gcgaccatgt tcttcaagtt ctgttccact ttcactttta aaactacttc cgcaggccat
      601 tcgtcttctt gagctttctc caattccaga aaattattgc cgcgtcgggc cactcttctt
      661 gtttttagac tatctcccag tgaacttgtc agagaaacag atttgagtga ttgaaaaggg
      721 cggtcgattg gcagactgag cctgatttta tcaaacgacg ttaggcgcca ttcttccagc
      781 aatttcgcaa gttgcagtcg ccatgtaaac tcaatggttc gcggcgcaag ttgggatcga
      841 acccagctcc ctgccggtgc aattgcaagc gctcgcgttg tatctccctt ctgccagtaa
      901 agccatgcaa ggttctcggg ataccacgag ccgatagtct ttcgcgctgc cttcagtctt
      961 ttttgcgcgt cctgattctt tgcgatagca gcatcccacc tctgcaattt ctccagacac
     1021 tggattgtct ctttataagg aagttcatca tttgcatcat tgagagccca ggccctgttc
     1081 aaaaacggca gagcttccga cggctgattg agatacgcgt ttagagtata gccaatcatg
     1141 aacggtctgc tccaaacgtt cggatccagg cgatacgctt ctctaaagaa ttcgagcgct
     1201 ctgtctggct gtttctggtt gtagagtgcg tcgattcctc tgtcataggc cgcctgactc
     1261 tgaggctgcg acgcgagtga agagagacaa accaaaagaa gtagaagagc aaccttgatt
     1321 ctgatcatgc gatgtttgta gagcgcagcg cgtcaccatg caagtcaaaa acgatgaatt
     1381 tcacgcccgc gattttcgag cattttagct cgaccggagc gaagcacccc gtagaaagaa
     1441 gagatcatga aggcgtgcga tcgtaggata ccggcaatca gccaccgggg acaacttctg
     1501 gccacttcac tctaccattg ccggacgcgg cactgtgcct gatctgttct cgctcatggc
     1561 gtccgccgtg tacaatggag acgcggtccg cgcaaaacaa ctattagacg aaggagttcc
     1621 acccaatcac atcatgcccg aaaacctcgg acatagcttt ctaacattcg cctgtctaca
     1681 gggacacctg ccagtagtcc agatactgat cgaagcgggc gcccatataa accgaaaaga
     1741 ttcgtttcgc acttcgagcg gtaccgatgc gggatactat ccactaatgg ccggatgcaa
     1801 tgcgcgcgat catgcaccag aaatcgccaa ataccttctg gaacatggtg caaaagtcga
     1861 cctcgcccat ccttctggta gagacggcgc gattcatttt gcggccatgc gtggcgactc
     1921 ggcagtcgtg aaactgctgt tagacgaagg cgtaaatgtt aatcagccgg gtagctatgg
     1981 atcgactcct ctgacgcttg ccttgcacat gaattcgaca tccaacctcg taagaatgct
     2041 tctaaacgcg ggcgcggaga tcgattcgtc tcccaataaa ccaaacccac ttttcgaatt
     2101 gattgcgggc tggctggatg aagcggacct ggcccgtctg ttcatcgatc acggagtgcc
     2161 gaccacagac gcttcgggca gcgctatcgt tcaagcggcc tcgcgcggca agactgccat
     2221 cctgaagact ttgcttgaaa acaaaccatt gtcatcaccc gacaccacgg atgctgtgct
     2281 cgcagcgatg aaggggcagc attttgatgc agtggagttt cttctgcgcc acggcgccga
     2341 tccgaatgca gccgagcgat ttgagggcac actcctcaga caggccacca gagcgggaaa
     2401 tacaaaactt gtttctctga ttcgtctcaa gcaaaaagaa aaatcggaaa ccagaaatac
     2461 cggcgctatg cttgccgcgg cagaaaacgg cgatcttgcg atgatgcgtt tgctcttcga
     2521 atcgggactt ccgatcgatg aagttcacga tggtgaagtc tggggttcga aaaaagaaac
     2581 tccactgatg agagccgctg cgcgcggaag aatggaagca gtagagtatc taatgttgca
     2641 cggagcaagc gtagatgcag tcgacgaaaa aggaaacaca ccgctcatgt atgcggccgc
     2701 ggcgggccag acagaagcca taggatatct tctcgatcac ggcgcttcca tcgactcaac
     2761 caaccattta aactggaatg cactcatgca agcgtgcttc accggaaaag agagtgctgc
     2821 aagactgctg ctcgaacgcg gttcgaggac agatccaatc gatctggaaa aaggggctac
     2881 agcactttcc ctggcaagga tgtcagagaa caccaacctt gtgaaaatgc tcgaagaaag
     2941 aggagtgacg gatagactcc ggcgaagaag agcctcgaat cagcaatatg cgggcattgc
     3001 tgattgcgat atttgcacct acatggcgcc ccgccaggag ttgactcatt cctacactcc
     3061 ggaaaaaaca gaggacctgc tcatcattca cgaagaagga agcgtggact acaaacaaga
     3121 aatcacgcga atgataaaga aatgcaaaga atgcggcacc tactactatc actatcactt
     3181 cattgataca gaagattcga taggaggcgc gggaccgatc tgctcgcact acctgattcg
     3241 actcaacctg ctctgggccc tggatttgtt ttcgaaatta gaaaagaccg gggaactggg
     3301 cgaattgaat caacgataca gtcagctgat tgaatcgttc gccattctgt tgcgggcaga
     3361 aaaccatatc attccaccct atgtacttcc aacggtcgtg gaatcgctga atgatttcta
     3421 cgtcatgaaa agttcctgga attccattgt gactttgctt tgccatccaa ccatcgccgt
     3481 gcttactctt gaagatatct tgcgcgtttt cggagagaat gcgcgcgatg acacttaccc
     3541 acctttcacg ccttacagaa actttacgaa agatgctcac gataagatgc gaaaattgct
     3601 gaggaagaat cagaaggagt tcagagagca cttgaatcga attgcgcgaa gctgcgatca
     3661 aaagacctcc gacttttttc tgagaactgc acaagcatac aaaataggct agaaatgctg
     3721 gacgaatact gcgacacact ttccgatccc gcgctacttg agatcgcact tcgcctgaca
     3781 gaactttctt tgccagtctg ggacaaacac ttctcttccc acccggaaga agagagaaaa
     3841 ctggctcgag caatgaacga agcgacccgc gtgcccggcg caagaacact ggatgcacat
     3901 tttccagaga cagctctctc tatgattgag aaatgctatg ccgattctaa aaccgggccc
     3961 tccgcgcttg aaaagcttcg cggcaaccct gaactcattc atcttttgtg cacttccatg
     4021 caacctctga cgaaccagga gtgggatgaa gcattcccgc gatccgttcg acttgtcttt
     4081 acgtcagtct ggaacatact gagctggctt gtgtatactc agtacagctc atcagatgaa
     4141 aaccatatct atgtggccat caatcaagca gcggacgcgt tgttgagcga aggactcttc
     4201 tctgtggaac agatcaatgc gattctggca gagtacaaaa acagaatcta tccaaatgaa
     4261 atcaatctgt acccgtcaag gaccgacaga agcaagaatt cttctgcaat gcagggattc
     4321 gctgaaaacg acgcgtatac tttcatcgca accggagagc gcgctccggg aaatgctcca
     4381 gcggattcgc agcggcgcct gatactcagg caaatgaagg aagaaaacaa aagcatctgg
     4441 gattctgatt atgagtactt caacggaatc tcaaggacct attcgtacaa tcacgaacag
     4501 gaaagctact ggatcaccga aatagacatc atagcagcaa gcttctgcaa cgagcgcgcg
     4561 ctcaccgaag aggaaatgct ggccgctctg agcgattttt cgctccatca tttacgcgaa
     4621 agtggtttta ctttctgagg cctctatttc gtggaagcac gatagagttt gctcccggaa
     4681 ataatgaaca gatcatctcc gatcgatgct gcgccttcca cttcagtcca accgcctccg
     4741 ccgatctctg tgtactttcc tgcgcggtca atcttataca acttgttgcc gtccacagcc
     4801 cacaaagaac cacccgcagc aacaaaggca gaagtagaac tccatcctcc atctccgagc
     4861 tcagtgtatt ttccagtctt gtctgttctg tagagcttgc cgctgtctat gatccagagt
     4921 gctccgtcaa aagcaaccat cgcactgctc ttactccatc cgccttcccc cacttctaca
     4981 tattttccat tcttgtctac tttgtagaga gtgccgttgt caatgatcca gagtgagcca
     5041 tcgagcgtag tcataagcct ggtcttgctc cagccactgg caacctccgc atactttcca
     5101 ttcttatcca cgcgatagag tttcccgccg tctatgatcc acagagctcc gtccatcccg
     5161 cacatcgcag aagtttcact ccagcctccg tcacccagtt cagtgtattt tccgctcgag
     5221 tctgttctgt aaagtttgcc tccgtgaacg gcataaatgg cgccgtcgag tgcagtcatc
     5281 gcacgcgtac catcccatcc accgccaagc tctttgtagt ccccgggctt tgcgatcaga
     5341 cctgagagag atgcgaagaa tagaatgcaa atggcggatg cgaatttcat ttctctagtc
     5401 cagaccagga gcaagaagga gtcaaacggg ttttgatcac ctggaataaa tcccgtatct
     5461 ctcggagaaa gccggaggca gagacatctc ttttaaaaaa gcaattgtcc gggaggccac
     5521 ttccggcgaa aaggactcaa atgcgtccca ccatatttgt aatggacctg gaaggatgct
     5581 tgatcccgga aatctggcta cacatcgcag acaagactgg aatcgcagat ctcagaaaga
     5641 caaccaggga cgtggccgac taccgggagc tcatgaacgg caggcttgcg gttctagatc
     5701 ggcacaaaat caaactctca gacattcagg ccatgctcgc cgatcttgga attttagaag
     5761 gcgcgcgtga ctttgtagaa tgggtgcgcg aaaaaacaga attcattatc ctttcagata
     5821 cgtatgtgga attcgcaaag cctctcatga agggccttgg gaaccccact ctattttgcc
     5881 atcgcctcac tacaaacgca gcaggacaga tagacggcta tgctcttcgc agcgaagaca
     5941 gcaaagcgga cattgtgcgg gcacttaaag gaaatggatt tcgcattatg gccgctggcg
     6001 attctttcaa cgactccaac atgctgatcc aggcggataa cgccagatgg tttcgcgcgc
     6061 ctgcatccat tgtagagaaa tttccgcagt tccccgcgct cactgaatac caggatctta
     6121 agaatgcgat ttcgcaattc ttgctttgag tgcaggatcc atcatccgca gaacatcccc
     6181 aacaatcgcc acagcaggaa aacgaacaga cctggatacc tcaggatccc tggcgtgcga
     6241 gattgtagtg agagtgactg actcttccgg taagctgata ttggacacaa gtgcgatgga
     6301 agtattttct tctcttccgg cctctatcaa tgactgacag atccgggaaa gatgatgctg
     6361 acccatatag aagacaagtg tcaaagagga actggccagg tacttccagt catgatgtgg
     6421 aaagccatcg tccgattctc tatccgcacg actgtatccg gataagaaaa caacagacgc
     6481 ccccagatcg cgatgtgtga gagggctctg taagagcgcg gaggccgcgg tggcagcggt
     6541 gacgccggga atcacctcaa gctcgattcc gtgctcacgc aagaattgaa tctcctcacc
     6601 acctcgccca aaaataaacg gatctccacc cttgagccta accaccgtgt gatgcttcga
     6661 tgcttcttcc agaatcatct cattgatctg gtcctgcgtg taactatggc tatgcgctct
     6721 cttacctaca aatattcttc gcgcattggg ggaagcgaac ttgagaatct cgggcccgat
     6781 gagactgtcg tataaaatca catctgcaga ttgaatgatc ttgcttgcct tgatcgtaag
     6841 aagatctgga tcaccgggac cggcgcccac gagataaacc tttccctttg gatgttgtcc
     6901 ttcttccagg cccacgagtg taaacttttc cagaggatca caccacgcac tcacttttaa
     6961 gaatcaacct gattcactct gacttgacag gcggcccaaa caagaacgga tgcaaggatg
     7021 ggtgagaacc gcactccgtt ttcggaagag caaatcaaag gagtctatcg cgccatccac
     7081 gagcgccgcg atatacgcgc gtatcgccct gaggcagtcg aagacggagt actccaacgc
     7141 cttctagaag cggctcatca tgcgccatcg gttggcttca tgcagccgtg gaacttcata
     7201 ctcataacag acaaagcgag gagacgcgcc ctctatgaac attttctaca gtgcaatgaa
     7261 gaagcatctc agcactttga aggagaacga aaactcaaat acgattccct caaacttcag
     7321 gggattctgg atgcaccgat caacatagta ttcacctgcg atcgatctcg cggaggaaaa
     7381 cacgtactgg gaagaaacac gatcccggat acggatatat acagcacatg ccttgccatt
     7441 gaaaacttct ggctggctgc gagagcggaa ggtttgggcg tgggctggat gagcatccac
     7501 aaaatcgaaa cgctccattc cattctagaa attccagcgc atgttctgcc tgtggcctat
     7561 ctcaccctgg gctatccagt cagttttcca gatgcacctt tgctcgagtc gaccgggtgg
     7621 agggcgcgcg aagacctgac aaacctggtg tacgaaaatg tctggggcca gctcaaagag
     7681 ttcgcgccca aagagcagcc tccgaaggcc ggaggccaca ttcagacaag cacggaaagc
     7741 agtgccggta gaagcatcgg gcgcgaggca gaaacagtgc gccatcggct ggaccatttg
     7801 accaagccca gaggctctct gggggatctc gaagattgga ttgtgcacct tgccgcgatt
     7861 caaaagagaa atgtgccgtt atgcgatcat ccccacatac tgctttttgc gggagatcat
     7921 gggattgcag cacaccatat cagcgcctac gggcagcacg tgaccgcaca gatgatgtat
     7981 cagtatgtag ccggcacagc tgctatcaat tctatcgcgc gtgcaaatgg aattgctctg
     8041 tccatatatg acgtcggagt gaaccacgat tttggaaatg cggagggaat ccaacatcgt
     8101 aaggtgaaaa tgggaacgcg cgactttcaa atagattgtg ctatgacccc ggatgaatgt
     8161 agagcggcct acgaacacgg acgcagctcg gttctggaac taccggacac atgctctatc
     8221 attggcctgg gtgaaatggg aatcggaaat actaccgcag catctgccat cgcctgcgca
     8281 ttgttaggtg attctgcgga aaacctcacg ggcgctggca ctggaatcgg accagacacc
     8341 agaaagagga aaatcgaaat catagaaaaa ggactctcac ttcataggca gcaaattgcc
     8401 tccccgatgg atgcgattgc ccgcctcgga ggttacgaaa tcgcaagcct ggccggagcc
     8461 atagagggcg ccttcgaaaa gaacataccc gtggtcctgg acggattcat cacaggtgtg
     8521 gcagcactcg ccgccgtgcg acacagacct gaagtgcatt ctgttttgat agccggacat
     8581 tcttcaaagg aaaaggcaca cgcgcaaatt ctcgcaaaac tgaacctgcg acccatcttg
     8641 aatctaaacc tgcggctcgg cgaaggctca ggagcagccc tggccatggg actcatcgca
     8701 aacgcatgcc ggctatattc ggacatgaaa accttcgagg aagcgaatat tatctttgct
     8761 gaaaatgaac aggcgcgact ttagagcgtc agagaaatac atgaaatcat tgagcacccc
     8821 catccagttc gtcgtcgcag tgctttgcgc cgcgtgcgtg acatctctcc aggctcaatc
     8881 cgggctcaga attgaggcaa gctatgcgag catagaacac tcgatggatt caaattcaac
     8941 tcatgagacg ttcactctgg caggcagcaa actcacctac aactacgaat acggcggcta
     9001 tcaccccggc ccgaactttg agagaaagag aacggacagc aagaaagtaa atcctgaaga
     9061 tctgcacaag tttgttatgg aaaagaagct caatcgatct ctcaaccgaa aggttgtttc
     9121 ttcgagcccg catgccaggg aggttcacct gcaccttgac attcgcatgg gtgcagagac
     9181 attcgttttg aacttcgacg gaacaagacc ggatgcgggg gcagacaaag acttcgacaa
     9241 cgctcttgca ttctgcgatc taatgcgatc catcgtatcc aatccttagt atcgaaaaac
     9301 tattgcgcgc gaagacgtgc aaggaagcgt cacccatgcg aattctaatc gtgggcgccg
     9361 gaagtgtatc aggcaaatcg gcattgcagg cagcaaaagc ggccggggcc acagtcatag
     9421 ccaccaccag caggaaagaa aaccttccgg gcgccgatca cacgattcat gggatagacc
     9481 tggaaaatcc agcggccgta gatctgattc tcgcagatcg attcttaacc caacaaccac
     9541 tcgattacat tgcctacatc ccggccagag gtgcagttgg aatttctctg gaagaagcgg
     9601 aagcgtctat ggtggaacca agcctttcgt attctgtgcg tccctatctc aaactgcaca
     9661 aggcccttca tccgaaaaag acaattgcgc tttccggttt cattacgatg gaacctttgc
     9721 tgaagatcta cggagccatg tgcttcacca aaattgccat ggaacaactt gcagtggatc
     9781 atccaaaaga tcttcagatc attcgaattg gaatgtttca cagcaattca gtcagaggca
     9841 tcatgattct cgcacagaga cgatttatgc gagatccttc ttaccagcct gaatggcgcg
     9901 cagaatggaa agcatccgga aagaaattcc cggagttctt ctacgcgaag aactacaaat
     9961 gcgaagaaga aacgtacaga atccacggac agggaaaacc atttcgcccc acggaacctt
    10021 ctgatatcgc agagggcttt cgcctggca
//
LOCUS       DAOQJL010000077        10020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3537_length_10020_cov_5.024486, whole genome shotgun sequence.
ACCESSION   DAOQJL010000077 DAOQJL010000000
VERSION     DAOQJL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_10020_cov_5.024486"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLG78_05205"
     CDS             complement(<1..565)
                     /locus_tag="PLG78_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22539.1"
                     /translation="MQPIRFYLILTGVLLVGELTAQVPVKNQPPAGWCSDIRAQVRKE
                     APAVPALLALKATAPTADLQKALTSSDWIIIGDYSYPEQKVNPYYGVSSEQMDFHRFA
                     ADGGMLSFSCYIFKDGKEKTTHINFTAPDTTWNVKKIGTAYYIEEIAHGEKAYFPIIQ
                     FKDNILVVDISQFGKIGEKIDFRSVLIA"
     gene            677..1726
                     /locus_tag="PLG78_05210"
     CDS             677..1726
                     /locus_tag="PLG78_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE22540.1"
                     /translation="MKIVQTLTAIVFTSVVCYCGYKPSEAAKTSMLIVFVTGDAFVVS
                     QNKPEVPATVGMIVRENDVIRTESGSVDLQTVHGNAVRIREMTTLTVAAIHSKGETKL
                     GLKNGAVLAKVQKASKEESFIISTPTAVAGVRGTTFSVEIGPDARSSVRVIDGKVGLA
                     PRVAILESMTPEQIKADPTLQKLSTLQSQETVLEDKTQGTLNANLEKQLIRVSETRKP
                     EEIKAEQSVETKTVEIPIRELAESRTLVSVKPQLMDRAIKGDAQAVQEIQSEREMAKE
                     RALDQIMKDASTIELKSEDAIRTRYSRFELITLNSGEKIRGAVIAQTDSSLVVHTTSG
                     VRKIPRAELNFQEPL"
     gene            complement(1748..2689)
                     /locus_tag="PLG78_05215"
     CDS             complement(1748..2689)
                     /locus_tag="PLG78_05215"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE22541.1"
                     /translation="MEKNEQIFIVRPDAEGTRLDQWLAAQMASSEEAPSRMQIQRWIK
                     AGLVEGQGTLNPARRIEAGESFTVKPAISEKATDLEPIDLNLKILYQDDELAVIHKPA
                     GIAVHPGPGDGGITLASGLIHAFSELKQGEWEGRPGIVHRLDKPTEGVLLVAKNVKAH
                     RLLSGQFLRREVKKTYAAWLLACPPAEEGEINAAIKRHPKDRLRMRIDPTGRHAITRY
                     KVTRTRVSRRGRKFALVEVDLLTGRTHQIRVHMAHIGCPIVGDEIYSRSSAEFKKFGL
                     LLFAQTLEFKHPTNGSTMRFSLDLPERFDRFENSLEG"
     gene            2803..3339
                     /locus_tag="PLG78_05220"
     CDS             2803..3339
                     /locus_tag="PLG78_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE22542.1"
                     /translation="MKVSNLFRIASIALFTVAIAACSSSQSTSGNNTNSNDRSGSKER
                     GGVDMGGALSSLSYSPNGQGFAYKSTYVNPTEFNTWATQFKPQIETALNALAPGFKLQ
                     ITGHTCAIGPRDGDGAHLGNVYYSTERAKAVYQALINAGLPKDKLTYKGIANDEPLPG
                     AAPKDQKNRRVTFKIVQG"
     gene            3397..4203
                     /locus_tag="PLG78_05225"
     CDS             3397..4203
                     /locus_tag="PLG78_05225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22543.1"
                     /translation="MFHNQTAVITGGSSGIGLALAKELRLRGARVVLISRSGVKLKKA
                     QTVLAEIPGDPVNVFRCDVRNYRQLRRVFQEIEKSGPVSILINSAGMVHPGKFETIPV
                     SLFRRTMAANVLGTVHACRAAAPAMMSRHAGTIVNIGSLAGMIGVFGYTAYSASKFAV
                     HGFTQALRAEMKPHGIHVMLAAPPDTDTPMLHEERKLLPRESAAINASGGLLSPKAVA
                     LAIIRGIEKRRSIVVPGFEGKFAYYASRFIPGIVDAVMDHKIRKAQSGKP"
     gene            4247..4495
                     /locus_tag="PLG78_05230"
     CDS             4247..4495
                     /locus_tag="PLG78_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22544.1"
                     /translation="MAKLLLSLPLILGLLSYCSAQSCDAKRQQSENCKVASLPLLATC
                     PSASTTSNQNTCYAAWAAAIVACDAIVPPYCAKPSSDD"
     gene            complement(4529..6865)
                     /locus_tag="PLG78_05235"
     CDS             complement(4529..6865)
                     /locus_tag="PLG78_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNE22545.1"
                     /translation="MTAPSILITQCLQNDFVRLIDRYEALPNLLHVGYDESMRLLGER
                     MEEGPVRSMLHWAYERSPEELAIIHIRDWHNPSDPAQKDHLKQFGPHCVQDTEGARFV
                     FEESLDPARHTIVNASGLNDFVDTTLEEVLRPYRGKPVRVGIAGVWTEAKVTFLAYDL
                     RTRYPEFEIAVASPLTASSSRQMHFIALDQLKSILGVRVFSSLGGFTEFLTGSIPDIG
                     SRIPFREGLLHLDGSYSVSETDKSILTYLFRDCKKVELKCLDGGFSGNVVLKARSVDV
                     LGHPQVPTVIKIGARDPIAQERTSFERIEEVLGNNAPQIVDFAEIGERGAIKYRYAAM
                     LDGPVHVFQDLYESIEEEIASGKKHLSEETLKPVFDVLDTVFLAQLGRLYQAGEPEKV
                     DLLQYYDFSTRYAPGVRRRITALIGGNPEDIGEVFPGRNIENVCPFYEKDLEHLHSTR
                     TSARMAFVHGDLNGRNIVLDPSHNVWMIDFFHAHRGHILRDLLKLENDIFYIFTKIAS
                     ISELEEAVRVTDFFLSQPDLGVPPLPGALSISNPGLKMAALTVAHMRSYYPALVDKDR
                     EPVQMQIGLLRYAMHTISFDECNEFQKKWALASGSALITKIKESFARSSRLRIDFMET
                     GEPGKIGMTILPGRKDRERVLSEDIAAIKESKIASVLCLITENEFSEYGVPDLKQEYE
                     KAGLATRYLPILDQAIPTREELSSALSWVHDELKQSKNVLVHCVGGLGRSGLAAAAYL
                     ILEKNMSPDRAIETVREARGRRAIETRKQEEFLESLTR"
     gene            complement(6876..7643)
                     /locus_tag="PLG78_05240"
     CDS             complement(6876..7643)
                     /locus_tag="PLG78_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE22546.1"
                     /translation="MLIQESITDFIAEIKLQINETNSFTPDGFAALEGALTKMAADNS
                     VRVLILSSARPDFFSNGLDPHSVHGATPEQIQKLISSFFSVLKKIFLFPVPVIAAING
                     HAVGYGAMLGLMSDFRLLVDKGARVSFPELNIGVSLPIFVTTALEDLVGLRATRDLLF
                     TGLAPKPPEALALGLVDELVEKEKLADRARALGKKIASLPKNATRAQKGIIRHRFAQN
                     LDRILEEDIKQTGGLLNSPEAREGFAAMVEKRRPKFD"
     gene            complement(7674..9665)
                     /locus_tag="PLG78_05245"
     CDS             complement(7674..9665)
                     /locus_tag="PLG78_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22547.1"
                     /translation="MRRTVFFVFLIAFPLLSGCTFDSTESLSGDWNAALNFLPGSRDP
                     FAKLEWKAIGTPAKLSRESGIDTFRYRGWIILQRPLPLDLGQKALEGKLALHIGRVSD
                     AAIIYLDDTIAAKIGNESPYQSGFRHFTGSLPWKGTSPPKVLRIAMLATEQFPATISG
                     LEIQGGSTEDVFAHANYLELISCFFVAIYLSIGCYHMILGLRRLEDLYNIHFGLFALL
                     LAAFYAFQTYTFETVSGNQVLLRERLEETVLFLLGPPLLFFLSDYFYGTLSRFGLLAQ
                     LFFTGMVIAVWFSDYRHLMVLRDVWHLMAFAVMVYVMFYIARECYEKKRDAMYMAPGI
                     GLLMLTGIHDIMVARGLLYSIPGTSKFSFLILNAGLAVILANRVIRLQQRVTDLNAHL
                     EQRVESRTIELAAALSDLQNKDKRIQNEMELAREIQLGILPEMPLLWKEFRITARYQS
                     MRKIGGDFYDVQQTRDGKLAILMADVSGHGIPASLITTMTKISFTTALQHSSSPAEVL
                     RAINDEILAHITSHQYLTAALIVISENGDALYASGGHCPAVLIGKSDIRLVSSAGSIL
                     GAMREAPVAIEEIPVEIKSGDRLLLYTDGITEARHNRENFGMDRLRQICAEVGQEDGE
                     TFLDSVLSAVSRFAQKADPDDDISLLLLEHTAGVPVRNE"
     gene            9697..>10020
                     /locus_tag="PLG78_05250"
     CDS             9697..>10020
                     /locus_tag="PLG78_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22548.1"
                     /translation="MREKTMNNPLSRLPVVGKWFREPVAGRVYHGIDSIRRRASLFHY
                     PLRAFFRAVARTQHSEIQAIGLENIPPDGAVFLVGNHPNSYVDFLNLSVVLRHPVATA
                     AKDTIT"
BASE COUNT         2403 a         2476 c         2563 g         2578 t
ORIGIN      
        1 ctgcaatcaa cacggatctg aaatctatct tctctccaat ttttccaaat tgcgagatat
       61 ccaccacgag aatgttgtcc ttgaattgaa tgataggaaa atatgctttt tctccgtgtg
      121 cgatttcttc tatgtagtag gccgttccga ttttcttcac gttccatgtg gtgtccggag
      181 ctgtgaagtt gatgtgtgtt gttttttctt ttccatcttt gaagatgtag caagaaaatg
      241 aaagcatgcc tccgtctgcc gcgaagcgat gaaagtccat ttgttccgag cttactccgt
      301 agtaagggtt gactttttgt tcaggataag agtagtcccc gatgataatc cagtcagatg
      361 atgtgagtgc tttttgcaaa tctgcagtgg gtgcggttgc cttcagcgca agaagagctg
      421 gaaccgccgg agcttccttt cgaacttgag cgcggatgtc agagcaccag ccagcgggcg
      481 gctggttctt aacaggcacc tgagctgtga gttctccaac gagaagcact cctgtgagaa
      541 tcaagtagaa tcgaatcggc tgcatgcgtc atggagactc attcttcggg gttcgcatcc
      601 cttttttccg gactaattct ggagaaaatc tggcaaaaaa ccggcccact gtttgttgtg
      661 tagatggagc aatcatatga aaatcgtcca gaccctgacc gcgatcgttt ttacctctgt
      721 cgtctgctat tgtggatata agccatcgga ggcagcgaaa acatccatgt tgattgtatt
      781 cgtgactgga gatgcctttg tagtctcgca gaataagccg gaggttcccg caactgtggg
      841 aatgattgtg agagaaaatg acgttattcg cacagaaagc ggatccgtag accttcagac
      901 cgtgcatggc aatgctgttc gcatcagaga aatgacaaca ttgactgtcg ctgcgatcca
      961 ttctaaggga gaaacaaagc tcggtttaaa aaatggcgct gtgcttgcga aagtgcaaaa
     1021 ggcaagcaag gaagaatcct tcatcatttc cactccaact gcagtggccg gtgttcgcgg
     1081 tactactttc tccgttgaaa tcgggccgga tgcaaggtct tccgttcgag tgatcgatgg
     1141 aaaggtcggg cttgctccgc gagttgctat tcttgaatcc atgactccag agcaaatcaa
     1201 agcggatcca acacttcaaa agctttctac tctgcaatct caggaaaccg tcctcgaaga
     1261 caagacacag ggcactctca atgcgaatct ggaaaagcaa ctgatccgtg tatctgaaac
     1321 tcgcaaaccg gaagaaatca aagcagaaca gagtgtggag acaaagactg ttgaaattcc
     1381 aatccgtgaa ctggcagaat cgcgcactct tgtatctgtg aagccacagt tgatggatcg
     1441 tgccatcaaa ggagatgctc aggctgttca ggaaattcag agcgaacgcg aaatggcaaa
     1501 agagcgtgct ctcgatcaaa tcatgaagga tgcatccacc atcgaactga aaagcgaaga
     1561 tgcgattcgc acacgttatt cgcgctttga attgattact ctaaacagcg gggaaaagat
     1621 tcgcggagcg gtgattgcgc agacagacag ttccctggta gtccacacta cgtccggtgt
     1681 tcgcaagatt ccacgtgctg agttgaattt ccaggaacct ctgtaagagg tcacggcgct
     1741 ggaaggatca accttccagc gaattctcga aacgatcaaa tcgctccggg agatccaggc
     1801 taaaccgcat agtactgccg ttggtcgggt gtttgaactc cagcgtttgc gcaaataaga
     1861 gaagtccaaa tttcttaaac tctgcgctgc tgcgagaata gatttcatct ccaacgatag
     1921 gacacccaat gtgggccatg tgcactctga tttgatgagt gcgtcctgtt agtagatcca
     1981 cttctacgag tgcgaacttt ctaccacgac gcgaaactct tgtgcgagtt actttgtatc
     2041 gcgtgattgc atgacgtcct gttggatcaa ttcgcatgcg aagacgatct ttcggatgtc
     2101 tcttgatggc tgcgttgatt tccccctctt ctgccggtgg acatgcaagg agccaggcag
     2161 cataagtctt ttttacttcg cgtctgagga attggccaga gagaagtctg tgtgctttca
     2221 cattcttcgc cacaagaaga acaccttctg ttggtttgtc gaggcgatga acaattccgg
     2281 gtctaccttc ccattcgcct tgcttcagtt cgctgaaagc atggatgagg ccgcttgcca
     2341 gagtgattcc gccgtctccc ggtccaggat gcaccgcgat tccggcgggc ttgtgaatga
     2401 ctgcgagttc gtcgtcctgg tagagaatct tcaggtttag atcgattggc tccagatcgg
     2461 tcgctttttc cgatatagca ggctttacag tgaagctttc gccggcttct atcctacgcg
     2521 ctgggtttag agttccttga ccttcgacaa gacctgcttt gatccatcgc tggatctgca
     2581 ttcttgaggg cgcctcttcg ctcgaagcca tttgagcggc cagccactgg tcgagcctgg
     2641 ttccctctgc atcggggcgc acgatgaaaa tttgttcatt tttttccact ggatttacgc
     2701 tgttccaact ccttcaccgg gcccctgttt gacgcttaaa aaaagatttc catgcctgat
     2761 tcgggatttt aaaatgactc atataccatg aggtgcaaaa ccatgaaagt ttccaattta
     2821 tttcgcatcg cttccattgc tcttttcact gtagcaattg ctgcttgcag cagttcccag
     2881 agcacgagcg gcaataacac aaattcaaat gaccgtagcg gatccaaaga acgcggcggt
     2941 gttgatatgg gcggggcgct tagcagtctg tcgtattctc caaacggaca gggcttcgct
     3001 tacaaatcaa cctacgtaaa cccaactgaa ttcaacacct gggcaacaca gttcaaacca
     3061 caaatcgaaa ctgcactcaa cgcactggcc cctggattca aactccagat cactggccac
     3121 acttgcgcga tcggtcctcg tgatggagac ggggcacacc tgggtaacgt atactactct
     3181 actgagcgtg cgaaagcagt ttaccaggca ctgatcaacg caggtctgcc aaaagacaag
     3241 ctcacataca agggcattgc aaacgacgag cctcttccag gcgctgctcc aaaagatcag
     3301 aagaatcgtc gtgtaacgtt caaaatcgtt caaggctaat tctagtctac tgacgactca
     3361 ggaaaagcgg ctcaaagggc cgcttttttt ttgcctatgt ttcacaacca gacagcggtc
     3421 atcactggcg gatcgagcgg gattggcctg gcacttgcaa aagagcttcg cctgcgcggc
     3481 gcgcgcgtag ttctgatttc gcgtagcggt gtgaagctga agaaggcgca aacagttcta
     3541 gcggaaatcc cgggagatcc agtaaatgtc tttcgctgtg atgttcgtaa ctatcggcag
     3601 ctgcgtcgag tattccagga gatcgagaaa tccggaccag tcagcattct gatcaattcc
     3661 gcgggcatgg ttcatcctgg aaagttcgag actattcctg tttccctctt tcgtcgcaca
     3721 atggcggcta acgttcttgg aacggtgcat gcatgtcgtg cggcggcgcc tgccatgatg
     3781 tccagacatg caggaaccat tgtcaacatt ggctctctgg cagggatgat aggtgtcttt
     3841 ggctacactg catatagcgc ctccaagttt gcagtgcatg gcttcaccca ggcccttcgc
     3901 gcggagatga agccgcatgg cattcacgta atgctcgcag caccgccgga cacggacacc
     3961 cccatgctac acgaagaacg caagctgttg cccagagaat cggcagcgat caatgcatcc
     4021 ggtggacttc tatctccgaa ggcggtagcc ctcgccatca tccgaggaat tgaaaaacgc
     4081 cggtccattg ttgtgccagg ctttgaggga aaatttgcct actatgcgtc caggttcatt
     4141 ccaggaatcg tggatgctgt gatggatcac aagattcgca aagctcagtc aggcaaaccc
     4201 tgaatcaaaa ggcttgcagg ctcccggcag tgagctacag atgactatgg caaaactcct
     4261 gctgtcactt ccattgattc tgggtctgct gtcttattgc agcgcgcaga gctgtgatgc
     4321 aaagagacag cagtctgaaa actgcaaagt ggccagcctt ccacttctgg ctacctgtcc
     4381 atctgcttcg accacttcga atcagaacac atgttatgct gcctgggctg cggcgatcgt
     4441 tgcatgcgat gcaatcgttc ctccgtattg tgccaaacca tcctcagacg attgatggga
     4501 tcttgattgt tgatgggagc ttgatcgcct aacgggtcag gctttctaag aattcttcct
     4561 gtttgcgcgt ttcaatagcg cgtcttcctc ttgcttcccg gaccgtttct atggcgcgat
     4621 ccggactcat gtttttttcc aggattagat aggcggcggc agcaagacca gatcttccaa
     4681 ggcctccgac gcaatgcaca agcacattct ttgattgctt cagttcatca tgtacccagg
     4741 agagcgcgct cgacagttcc tctctggttg gaattgcctg gtctagaatg ggcagataac
     4801 gtgttgctaa tccggccttt tcgtattcct gtttaagatc tggaacacca tactccgaaa
     4861 attcgttttc agtgatgagg caaagaacag acgcgatctt actttccttg atcgccgcaa
     4921 tgtcctcaga aagaactctc tcgcgatctt tacgtccagg caatatggtc atcccaatct
     4981 ttcctggttc tcccgtttcc atgaaatcga tgcgaagacg actgctgcga gcgaagctct
     5041 ctttgatttt tgtgatcagc gcagaccccg acgccagcgc ccatttcttt tgaaattcgt
     5101 tgcattcatc aaatgagatg gtgtgcattg cgtagcgaag aaggccaatc tgcatctgca
     5161 ccggttcccg gtctttgtcc acgagagccg gatagtagga tcgcatatgg gcgacggtga
     5221 gtgccgccat cttgagtcca ggattcgaaa tcgaaagtgc gcctggtaac ggagggactc
     5281 ccagatctgg ttgtgatagg aagaagtccg tgactcgcac tgcttcttca agttcggaaa
     5341 tggacgcaat tttggtgaag atatagaaga tgtcgttttc cagcttcaag agatcgcgaa
     5401 ggatatggcc tctatgagcg tgaaagaaat cgatcatcca tacattgtgc gaaggatcga
     5461 gaacaatgtt gcgcccgttt aagtccccgt gaacgaaagc catccgcgca gatgtgcggg
     5521 tagaatgaag gtgttcgaga tctttttcat aaaacgggca cacgttttcg atatttcttc
     5581 ccgggaagac ttcgccaatg tcttcggggt ttccgccaat gagtgccgtg atccgccggc
     5641 gcacgcctgg agcgtagcgc gttgaaaaat catagtactg gagcaggtcc actttctcgg
     5701 gttctcccgc ctgatacagt cgtcccagct gggccagaaa cactgtgtcc agaacatcaa
     5761 atacaggctt cagagtttct tcgctcaggt gtttctttcc tgaagcaatt tcctcttcaa
     5821 tggattcgta gagatcctga aatacatgaa cgggcccatc gagcatggcc gcataacggt
     5881 atttgatagc acctcgttct ccgatttcgg cgaaatcgac aatctgaggt gcgttgttgc
     5941 caaggacttc ttcgattcgt tcaaacgacg ttctttcctg cgcgatcgga tcgcgtgctc
     6001 cgatcttgat cacagtggga acctggggat gacccagaac gtccacgctt cttgccttga
     6061 gcactacatt gccgctgaaa ccgccatcga gacatttgag ttctactttt ttacagtctc
     6121 taaacagata cgtaaggatg cttttgtctg tttcactgac gctgtagctt ccgtccaggt
     6181 ggagcaatcc ttcgcggaat gggattctac ttccaatgtc cggaatcgaa cctgtgagaa
     6241 attctgtgaa tcccccgagt gatgagaaca ctcgaacgcc caggatgctt ttgagctgat
     6301 cgagcgcaat gaagtgcatc tggcgagacg aagaagcagt gagtggactg gcgacagcga
     6361 tctcgaattc cggataacgg gtcctgagat catacgcaag aaatgttact tttgcttctg
     6421 tccatactcc tgcgatacca acacgaactg gtttcccgcg ataaggtcgc aatacttctt
     6481 cgagtgttgt gtctacaaaa tcgttcagtc cggatgcatt tacaatcgta tgacgagccg
     6541 ggtctagact ttcctcgaaa acaaagcgtg cgccttctgt atcctggaca cagtgtggtc
     6601 cgaattgttt gagatggtct ttttgggcgg gatcggatgg attgtgccag tcccggatgt
     6661 gaatgatggc cagctcttcc ggcgaacgtt cgtaagccca gtgtagcatg gagcgaaccg
     6721 gtccttcttc catccgttca ccaagaaggc gcatggattc gtcatagcct acgtgcagta
     6781 gattgggtaa agcctcatag cgatcgatga ggcgaacaaa atcattttgc aagcattgcg
     6841 taatgaggat cgatggagca gtcatgatgc taacgttagt cgaatttggg acgtcttttt
     6901 tctaccatgg cagcgaatcc ctcgcgtgcc tcgggagaat tgagcaggcc acctgtttgt
     6961 ttgatgtctt cttcgaggat gcgatccagg ttttgcgcga agcgatggcg aatgattcct
     7021 ttttgagctc gggtagcatt cttggggaga gatgcgattt tctttccgag agctcttgct
     7081 cggtccgcga gtttttcttt ttcaacgagt tcatctacaa gtcccagagc cagagcttcc
     7141 ggaggcttgg gcgcaagccc ggtgaacaat aggtcgcgag tggctctcag gccgaccaga
     7201 tcttcaagag ccgtggtcac aaagatggga aggctcactc cgatattcaa ttccgggaat
     7261 gaaacgcgag ctcctttgtc gactaacagc ctgaagtcgc tcatgagtcc tagcatggcg
     7321 ccgtagccca ccgcatgtcc gtttatggcg gcgatcacgg gcactggaaa tagaaagatc
     7381 ttcttaagca cagaaaagaa actcgatatg agtttttgaa tttgttcggg cgttgcacca
     7441 tgcacagagt gcgggtccag tccattggaa aagaaatccg ggcgcgcgct tgatagaatg
     7501 aggacgcgaa ccgaattgtc cgccgccatc tttgtgagcg caccctcaag tgcggcaaat
     7561 ccgtcgggag taaaagagtt cgtctcgttg atctggagtt tgatttctgc gatgaagtct
     7621 gtgatacttt cttgaatcag catggccgag cctcgcttcg ctgcaaaaga agtctattcg
     7681 tttcgcacgg gtacgccggc cgtgtgttcg aggagtaaaa ggctgatatc atcgtccgga
     7741 tccgcttttt gcgcaaatct ggaaactgca gatagaacgc tgtccagaaa ggtttctccg
     7801 tcttcctgtc cgacctccgc gcagatttgg cgaagtcgat ccattccgaa attttcccga
     7861 ttgtgcctgg cctctgtaat tccgtccgta taaagaagca gacgatcgcc ggacttgatt
     7921 tcgacgggga tttcttctat ggccacggga gcttcgcgca tcgcccccag gatggagccg
     7981 gcgctggata ctaaacgaat atccgatttt ccaatcaaca ccgccgggca gtggcctccg
     8041 cttgcataca gcgcatctcc attttctgaa atcacaatca gtgctgccgt tagatactga
     8101 tggctggtga tatgcgccag gatctcatcg ttgattgcgc gaagaacttc tgccgggctg
     8161 cttgaatgct gaagcgctgt tgtaaatgag atcttggtca tagttgtaat gagagaagcc
     8221 gggattccgt gcccggaaac gtccgccatg aggatggcca gctttccatc tcgtgtttgt
     8281 tgcacatcat agaaatctcc gccaatcttc cgcatagact ggtaccttgc cgttatgcgg
     8341 aattccttcc acagaagagg catttcaggg agaataccga gttgaatctc tcgtgccagc
     8401 tccatttcat tctggatgcg tttgtctttg ttctgcaagt cggaaagagc ggccgcaagt
     8461 tcaatggttc tactttccac tctttgttcg agatgcgcgt ttaagtcagt gacgcgctgt
     8521 tgtagacgaa tgactcgatt tgcgaggatc actgcaagcc cggcgttcag gatcaggaat
     8581 gaaaacttac tggtccctgg aatggaatag agcaagcctc gcgccaccat gatgtcgtga
     8641 attcccgtaa gcatgagcaa gccgattcct ggtgccatgt acattgcatc gcgcttcttt
     8701 tcgtagcact ctctggctat gtagaacatc acataaacca tcacggcaaa tgccatcaag
     8761 tgccatacgt cgcgaagaac catcaggtgg cggtagtccg aaaaccatac ggcgatcacc
     8821 atacccgtga agaaaagttg tgccagtaaa ccaaatcgcg agagagttcc gtaaaaatag
     8881 tctgatagaa agaagaggag tggaggcccc aggagaaaca gaaccgtttc ttccaggcgt
     8941 tccctgagca atacttgatt tccacttact gtttcaaaag tgtatgtttg aaacgcgtag
     9001 aatgcagcga gcagaagcgc gaaaagtcca aagtgaatgt tgtatagatc ttcgagcctt
     9061 ctcagtccca ggatcatgtg atagcagcct atgctcaaat agatggccac aaagaagcag
     9121 ctaatgagtt ccagataatt cgcatgggcg aatacatctt ctgtggatcc tccctgaatt
     9181 tccaggcctg agatcgtggc cggaaactgt tcggtggcga gcatggcaat gcgcagaact
     9241 tttggcggag aagttccttt ccaggggagg ctgccggtga aatgtctgaa ccctgactgg
     9301 tagggcgatt cgtttccgat ctttgctgcg atggtgtcat ctagataaat gatggctgcg
     9361 tctgaaactc ttccgatgtg caaggcgagt ttgccctcca gggccttctg ccccaggtcc
     9421 aaaggcaagg gtctttgcag tatgatccaa cctctgtagc gaaatgtgtc tatgcccgat
     9481 tcgcgcgaaa gcttggcggg cgttccgatt gccttccact ccagtttcgc aaatggatct
     9541 cttgacccgg gcaaaaaatt aagggctgcg ttccagtcac ctgaaagcga ttcagtcgag
     9601 tcaaacgtgc agcccgaaag caatggaaat gcaataagaa agacgaaaaa aacagttctt
     9661 cgcatgttca aggttttgtt gtagttcttc cggggcatgc gagaaaagac catgaacaat
     9721 cctctgagcc ggctaccggt agtaggtaag tggttccgcg aaccggttgc gggccgagtc
     9781 tatcatggca ttgattcgat ccgcaggcgt gcaagtctgt ttcattatcc gcttcgagct
     9841 tttttcagag cggtggctcg cactcagcac agtgaaatcc aggcgattgg tcttgagaac
     9901 attcctccgg atggagcggt ctttctggta ggcaatcatc ccaattccta tgttgatttt
     9961 ttgaatttga gtgtggtcct ccgtcatcca gttgccactg cggccaaaga tacgattacg
//
LOCUS       DAOQJL010000078         9991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3549_length_9991_cov_4.295189, whole genome shotgun sequence.
ACCESSION   DAOQJL010000078 DAOQJL010000000
VERSION     DAOQJL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_9991_cov_4.295189"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLG78_05255"
     CDS             <1..731
                     /locus_tag="PLG78_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNE22549.1"
                     /translation="KQRKGYAVVKTLVSVSSPHRGSPVAKYILDLKPGVVSVVDALAQ
                     LFGNVIYGSGNDGYAAAKQLVYNDYSSTDGVTTGMKAFNTAYASYNTAVSNRLASVIT
                     AQQGLDVNPALYILSKGFYDIDGDGATCAVASDDCDNDGALGKGNGSKTDTDDDGLVG
                     VNSQQMGYRLKYSECFLCDDYVTQDTTLGYLTDLNNPTSAQQSSHADVLNQDHLDVTG
                     VGPDTMDENEFYAAIIDYMSANGG"
     gene            849..1895
                     /locus_tag="PLG78_05260"
     CDS             849..1895
                     /locus_tag="PLG78_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNE22550.1"
                     /translation="MKNKKLLFLGGGAAIIVVAIAAFLIPGRDPVRKKFEELESKTSK
                     YTPSESVLESIKMKDAGDGHLTYDGELTFDQTVALLRKKYGAKINHPLVQMRMLQELL
                     RYLQKKYPNNWVEYLHELLGAAFPELAQKLFTMSKNLYLYDKFLNDTRDKATKMGDGD
                     RDKFLWSKRYELFGSDADEIWAGEKKYNDVKNALNDIANNRTGTIKEKLSQFKDTLNK
                     TYGDTLPSVMESRRQNLLDGFVGAVQTDLQSMTPADRREAMHDIRSSMGMDDAAITRW
                     DALDAERDQRWSNGQIYTNERKQIISQYTGTDREQKLDDLRHKLFGDEADTIKSEEAS
                     GYFRFDGKQQYGIN"
     gene            1892..4126
                     /locus_tag="PLG78_05265"
     CDS             1892..4126
                     /locus_tag="PLG78_05265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22551.1"
                     /translation="MKPGTFWRLTWRLELFTHLIPVPSAIYFSAVAGGFPENEFILAI
                     NVGAIGGGLVVVLGVIWRYFRLQSIERRVAALDAGSLDEARARRLKIEILRYPRDEAI
                     VIILRWITGVPIVHILYIILNGLRISTHITIPFVFLLTTPISAVVYLFISEREVRPLL
                     LRESLAHIHVPSDAIPKISYYWRILVSFLAVGSMPTVMLGYVLFAYVLGAMPLEHPLM
                     HVAAIALMMFATILFASWVVVRAVRDGLSVTHGVLTKLGEGDLDVVSERTSADEMGDL
                     DFHLGRVIEKLRSMYSEIRNLNESLEERVRSRTEELKKTLDDVQALKVQQDGDYFLTS
                     LLLEPLASNNSSGSTVGVQFLVHQMKHFTFRKWTKDIGGDICIADRFELKGLPHTVVM
                     NADAMGKSIQGAGGALVLGSVFHAILSRTRIVAGVQNLTPEAWLKSAFIELQKVFESF
                     GGSMLASLVLSLVDEDTGLMYHLNAEHPGLVIYRGGEARFIPGREIFKKIGTNTGHDL
                     GVITVDTFQLRPGDVVIAGSDGRDDLHVASAEEGGRIINEDMNAFLTSVEEGDGELDA
                     IYRSLQRRGSFIDDVSLVRFAFKEDAAAEIQTDTTPVSEALSALRGRGDLRQAAAKAE
                     RLLIDHPHPVLYRQLLKTYIKLKEYAAACVPGQWLCLHHPRDLQSIFLASYAMKRTGE
                     YMAAAEQGERIRTRDPFALKNLVNLADVYRLMGNRDRAGKITAEILVIEPQNHHALKL
                     KSLL"
     gene            complement(4163..7516)
                     /locus_tag="PLG78_05270"
     CDS             complement(4163..7516)
                     /locus_tag="PLG78_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE22552.1"
                     /translation="MTAEKPNHPEIYQPKHHIRFVTAASLFDGHDASINIFRRLLQSY
                     GAEVIHLGHNRSVQDVVEAALCEDVQGIAISSYQGGHVEYFKYLKQLLKSRGADHIKI
                     YGGGGGVIVQEEIQDLESSGITKIFSPEDGRTLGLAGIISSMLEGTDFSPKDLGIIQG
                     DSTKPEVLGRLITMIEEGENPPIEPGEKSCPVLGITGPGGAGKSSLTDELLNRFLEAN
                     PHLRICVFAVDPTRKKTGGALLGDRIRINSLSLHPDQIFFRSLATRKSGGTVSDALSR
                     ILSVFKRLPFDLIIVETSGSGQSDAEITEIADVNLYAMTPEFGAPSQLEKIAMLDFAD
                     IVALNKGDRAGAKDALMHVRKQYQRNHNRFSEDPQEMPVYTTVAARFQDSGVTQLYSA
                     LVDLIAKKTGVSFAPIDRSSPDLDTTEILPAGRIRYLSDIASTVRGYLAEGEAESNKC
                     SDLQSLMRARELSGDNAALVSALDERIAHLRSSIKPEVLEWLSKWPATREAYSGNTLT
                     YFIRDKQISQELRTTTLSGLSLPLIALPKTESSRDLVRYAYRENLPGQFPFTASVFPF
                     RKQDEDPIRMFAGEGGPERTNTRFHYLTGGETKSFVRLSTAFDSVTLYGEDPGLRPDI
                     FGKIGNSGVSVPTLDDCKRLYSGFNLLDPGTSVSMTINGPAPVILAFFFNTAIDQEVE
                     RYLRAEGKLEETRKRIGEFYSARGLSVPAYDRSLPAGHNGTGLLLGMTGERMVDPETY
                     AKIKKSVLTGLRGTVQADILKEDQAQNTCIFSTQFALKMMGDIQQFFSANGVRNFYSV
                     SISGYHIAEAGANPITQLAFTLANGFTYVEYYRKRGIRVDDFAPNLSFFFSNGLDAEY
                     SVIGRAARRIWAVAMKRLYGAGERSQKLKYHIQTSGRSLHAMEIDFNDIRTTLQALYA
                     LYDNTNSLHTNAYDEAITTPTEHSVRRAMAIQLIINKELGLNKNQNPLQGSYIIEELT
                     DLVEEAVLAEFDRLTERGGVPGAMEAMYQRAKIQEESLHYETLKHSGELPIIGVNTFQ
                     GKQTPPGETRMELIRSTDDEKNQMIERVANLSRAFPEEGKHALDRLKRAAASGENVFE
                     ELLETVKVCSLGQITGALYSIGGSYRRNM"
     gene            complement(7513..8358)
                     /locus_tag="PLG78_05275"
     CDS             complement(7513..8358)
                     /locus_tag="PLG78_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22553.1"
                     /translation="MKGPGLLLALLFASALSAQTERTVTILAIADWKGDFAVDQKGRG
                     GLAAFLALKRRIEQKIAPRGGSVIAVHAGQFTQATLSTALEDSLKRPDPGLIQYVGFS
                     ALTFSKQERALLEKTSLDLPVVERECTPKAKCKTEIITANSTLKWWITGLDPLKDRKS
                     DALLRDLESSAHKADADFSILLLSEPQTEAIFAGQTGNVHSAVGELGRFLVIESGKKN
                     LFYRDPAGPYVCIVAGRSICRVDLTFRGSLIGVSQKFIDPNGASEPGEFIKPDPVLMD
                     QFFRP"
     gene            complement(8417..>9991)
                     /locus_tag="PLG78_05280"
     CDS             complement(8417..>9991)
                     /locus_tag="PLG78_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22554.1"
                     /translation="KFALLYVREARAIYGALDITAKVEEIENHFPELVRGSQMSGSGG
                     LAQAGGFDFQTIVKAAASISGEIQLEGLLGKLLRIVMENAGAQKAHLLLDQEGAVVVS
                     AAGNALTDQVTPVPNVPLEDYNLLPHSIVLLAERTRTSIVVDEAASDSRFRADPYILE
                     YKPRSVLCMPVVLQGKLSCILYLENNGAPGVFSPARLEALQVLSAQIATSLENAKLYS
                     NLERALEQEKLARQAQVEINEAISRFVPSEFLKLLGKENIVGVSLGDNIAREMTVLFS
                     DIRSFTSLSESMTPEQNFRFINSYLNQVGPVVREHHGFIDKYIGDAIMALFDSPDNAL
                     MSSIGMLRALEVWNHGRLSAGYAPVRTGIGLHTGDLMLGTIGEKSRMEGTVISDHVNL
                     ASRMEGLTKIYGTSLIISNRTQSLLKDPSRFSFRTLDRVQVKGKKEPVIVIEVLDGET
                     DSVRQLKEASRAKFDEAQKLYLSQKFENARSLFIEVLKSNPDDRAAGIFVRRCRLYME
                     KGVPEGWNGVEQMTTK"
BASE COUNT         2341 a         2425 c         2607 g         2618 t
ORIGIN      
        1 tcaagcaaag aaaaggatac gcagttgtga agactctagt atctgtatct tcgcctcaca
       61 gaggatcacc tgttgccaaa tacatcctcg atctgaagcc gggtgtggtg agcgtagtgg
      121 atgctctggc acagcttttc ggaaacgtga tctatggatc cggcaacgac ggatacgcgg
      181 cagccaagca gctggtgtac aatgactaca gctccacgga tggagtaacc accggtatga
      241 aagcgttcaa caccgcttat gcaagctaca atactgcggt gtccaatcgt ctggcttccg
      301 tgatcaccgc acagcaaggt ctcgatgtca atccagcgct ctacattttg agcaaaggtt
      361 tctatgacat agacggcgat ggagccacct gtgccgttgc ttctgacgac tgtgataacg
      421 acggcgcact tggaaaagga aacggatcca aaacagatac ggatgacgac ggtctcgtgg
      481 gtgtgaactc gcagcaaatg ggctatcgcc tcaagtattc tgaatgtttc ctgtgcgacg
      541 actacgtaac acaggacacg actcttggat atctgacgga tctgaacaat ccaacatccg
      601 cgcagcagag ctcacacgcc gacgtgctca accaggatca cctggatgta actggcgttg
      661 gacctgacac gatggacgaa aacgaatttt acgccgcgat cattgactac atgtccgcga
      721 acggtggcta agaaaagttt gctaaatttc gaaaccgcct cttcgcaggg gcggtttttt
      781 atttaccgcg ttatcccttc gcgcgcgata taccacagac attcaaacat gctgaatgca
      841 tcactattat gaaaaataag aaactactat tccttggtgg cggcgcagcc attatcgttg
      901 ttgccattgc ggcatttttg atccccggtc gcgatcctgt tcgcaagaaa tttgaagagc
      961 tcgagagtaa gaccagcaaa tacactccat ctgaatctgt gctcgaaagc atcaaaatga
     1021 aggatgcggg cgacggtcat ttgacttatg acggggagct tacttttgac cagacggttg
     1081 ctcttctccg gaagaagtac ggcgcgaaga tcaatcaccc cctcgtgcaa atgaggatgc
     1141 tgcaggagct tcttcgctat ttgcagaaga agtatccaaa caactgggtg gaatacctgc
     1201 acgagttact gggtgcggcc tttcctgaac tggctcagaa gctttttact atgtccaaga
     1261 acctgtattt gtatgacaaa ttcctgaacg atacacgaga caaagcaaca aagatggggg
     1321 acggggacag agacaagttc ctctggagca agcggtatga gctgttcgga tcggatgcgg
     1381 atgagatctg ggccggcgaa aaaaaataca atgatgtaaa aaatgctctc aacgacattg
     1441 caaacaatcg cacgggcaca atcaaggaga agctcagcca gttcaaggac acactgaaca
     1501 agacatatgg agatactctt cccagtgtca tggaatccag acgccagaat ctgctcgatg
     1561 gttttgttgg agcggttcaa acagatttgc agtctatgac acctgcggat cgcagagaag
     1621 caatgcacga catccgtagt tccatgggaa tggacgacgc tgctatcacg cgatgggacg
     1681 cgctcgacgc ggagcgcgat cagagatggt ccaacgggca gatttatacc aatgaacgta
     1741 agcagatcat ttcgcaatac actggaacag accgtgaaca gaagttagac gatcttcgtc
     1801 ataagctatt cggagacgaa gctgacacga tcaagagtga agaggcatct ggctactttc
     1861 gcttcgatgg aaagcagcag tacggcatca attgaagccg ggcacattct ggcgccttac
     1921 atggcgcctg gaacttttca ctcatctgat tcctgttccc agtgcgatct atttctccgc
     1981 agttgcggga ggatttccgg aaaacgaatt cattctggcc atcaacgtgg gcgccattgg
     2041 tggcggtctt gtggttgtgc tgggcgtcat ctggcgttac tttcgcctgc agtccattga
     2101 gcgcagagtc gcagccctcg acgctggatc tctcgatgaa gcgcgagccc gccgcctgaa
     2161 gatagaaata cttcgttatc cgcgagatga agcaatcgtg atcatcctgc gctggattac
     2221 aggcgtaccc attgttcaca tcctgtatat catactaaac ggtcttcgca tatccactca
     2281 catcacgatt ccattcgtat ttcttcttac cacgcccata tccgcggtgg tttatctgtt
     2341 catttcagag cgcgaagttc gacctctgct tttgcgcgaa agcctggctc acatacacgt
     2401 tccttccgat gccattccaa agataagcta ttactggcgc attcttgtct cctttcttgc
     2461 agtcggatcc atgccgaccg tgatgcttgg ctatgtgctc ttcgcgtacg ttctgggtgc
     2521 catgccactg gagcatcctt tgatgcacgt cgcggccatt gctctgatga tgtttgccac
     2581 cattttgttt gcttcctggg ttgtggtgcg agcggtgagg gatggacttt ccgtgactca
     2641 cggtgttctc accaaactgg gcgaaggtga tctcgatgta gtaagcgaga gaaccagtgc
     2701 agatgaaatg ggagatctgg acttccatct ggggcgcgtg atcgagaaac tgcgctctat
     2761 gtacagtgag attcgaaatc tcaacgagtc tctagaagaa cgcgtccgat cgcgcacgga
     2821 agagctaaag aagactctcg atgatgtgca ggcgctgaaa gtgcagcagg acggagacta
     2881 cttcctcaca tcccttcttc ttgaacctct tgcttctaat aattcaagcg gttctactgt
     2941 tggcgtgcag tttctggttc atcagatgaa gcatttcacg tttcgcaaat ggaccaagga
     3001 cattggcggt gatatttgca ttgcagatcg atttgaactg aagggattgc cgcacacagt
     3061 tgtcatgaat gcggatgcga tgggtaaatc cattcaggga gcaggcggtg ctcttgttct
     3121 tggttctgtc ttccatgcaa tcttgagtcg aacgcgcatt gttgcgggcg tccagaatct
     3181 taccccggaa gcatggttga agagtgcgtt catcgaattg cagaaagttt tcgaaagctt
     3241 tggtggttct atgcttgctt ctctcgttct ctccctcgtg gacgaagaca caggtctgat
     3301 gtatcatcta aatgcagaac atccgggtct tgtcatctat cgcggtggag aagctcgatt
     3361 cattcctggt cgagaaatat tcaagaaaat tggaacgaac actggacatg atctgggtgt
     3421 catcactgtg gatacttttc agctgcggcc cggagatgta gtgatcgcag gttccgacgg
     3481 tcgcgacgat cttcatgttg cgagtgcgga ggaaggcggc aggatcatca atgaagacat
     3541 gaatgccttt cttacaagcg tagaagaagg cgacggtgag ctggatgcca tttatcgctc
     3601 tttgcaacgc cgaggaagtt ttatcgatga cgtttctctg gttcgctttg cgtttaagga
     3661 agacgcggct gctgaaatac aaacagacac tacacctgtt tcagaagcat tgtccgcgct
     3721 tcgcgggcgt ggggatttaa gacaggcggc agcgaaagcg gagaggttgc tcatcgatca
     3781 tccgcatcca gttttgtaca gacagctttt gaagacctac ataaaactga aggaatacgc
     3841 ggcggcctgc gtgcccggtc agtggctctg tctgcatcat ccgcgtgatc tacagtctat
     3901 attccttgcg tcctatgcga tgaaacgcac gggcgaatac atggctgcgg cagagcaggg
     3961 agagaggatt agaacgcgag atccatttgc tctcaaaaac ctggtgaatc ttgcagacgt
     4021 ttacagattg atgggaaatc gcgatcgtgc gggaaaaatc acggcagaaa ttctagtcat
     4081 cgagcctcag aatcaccatg cattgaaact gaagtctctg ctctaagctt gcgcgaagct
     4141 ttgccctgga attcgttttg cgttacatgt ttcgacggta gcttccgcca atgctgtaga
     4201 gcgcgcccgt gatctgtccc agcgaacaaa ccttcactgt ttcgaggagc tcttcgaata
     4261 cgttttcgcc tgaggctgct gcgcgcttga gccggtcgag agcgtgtttg ccttcttccg
     4321 gaaaggcgcg cgaaagattc gccactctct caatcatctg attcttttcg tcatccgtgg
     4381 agcggatgag ttccatcctg gtttctccag gtggagtctg ttttccctga aatgtattta
     4441 cgccaatgat tggcaattcg cctgaatgct tgagcgtctc ataatggaga gactcttcct
     4501 ggatcttcgc acgctgatac atggcctcca tggctcctgg aactccacct ctttcagtga
     4561 gtctgtcaaa ttcagcaagc acagcttctt ccactagatc cgtcagttct tcaatgatgt
     4621 aggatccctg gagtggattc tgatttttgt tcagtcccag ttctttgttg atgatcagtt
     4681 gaatcgccat ggcccgccga actgaatgtt ctgtgggagt ggtgattgct tcatcgtatg
     4741 cattggtatg gaggctgttc gtattgtcat agagtgcata gagggcttgc agggtggttc
     4801 gaatatcatt gaagtcaatt tccatcgcat gcagagatct tccgctcgtc tggatgtgat
     4861 acttcagttt ctgcgacctt tctcccgcgc cgtagagacg cttcatcgcc acagcccaga
     4921 ttcttctggc tgcacggcca atcaccgaat actcagcgtc cagtccgtta gagaagaaga
     4981 agctgaggtt cggtgcgaag tcgtctaccc ggatgccgcg ctttctatag tattcaacgt
     5041 aggtaaaacc attcgcaagt gtgaatgcaa gctgcgtgat tggatttgcg cctgcttctg
     5101 cgatgtgata accggaaata gaaacagagt aaaagtttcg cactccgttt gcggagaaga
     5161 actgctgaat gtctcccatc atcttgagtg caaactgtgt ggagaaaata caggtgttct
     5221 gtgcctggtc ttctttgagt atgtccgcct gaacggttcc gcgaagtcct gtaagcacag
     5281 attttttgat cttcgcatag gtttcaggat ccaccattct ttctcctgtc attcccagta
     5341 gaagtccggt tccattgtgg cctgcgggaa gagagcgatc gtaggcgggc acagatagcc
     5401 cccgggcaga atagaattcg ccaatccgct ttctggtctc ttccagtttc ccttctgcgc
     5461 gcaggtatct ttccacttcc tgatcaatgg cagtattgaa gaagaatgcc agaatcacag
     5521 gtgcaggtcc gttgattgtc atgcttacac tggttccagg atcaagcaga ttgaaaccgg
     5581 aatagaggcg tttgcaatcg tccagagtag gaacgctgac tccggagttg ccaatttttc
     5641 caaagatatc cggcctgagc cctggatctt cgccgtataa cgttacagaa tcaaaagctg
     5701 tggagaggcg aacaaatgat tttgtctccc ctccagtcag atagtgaaat cgagtgttgg
     5761 tacgctcggg tccgccttcg cctgcgaaca tgcgaattgg atcctcatcc tgcttgcgaa
     5821 aggggaaaac agatgccgtg aatggaaact ggcccggaag gttttctctg tatgcatatc
     5881 gcacgaggtc gcggcttgat tccgtttttg gcagagcaat caaggggagg gaaagaccgg
     5941 atagagtggt agtccggagt tcctgggaaa tttgcttatc tctgatgaaa tacgtgaggg
     6001 tgtttccaga ataggcttcg cgcgtggcag gccatttaga aagccattcg agcacttccg
     6061 gcttgatgct ggatctgaga tgtgcgattc tttcgtcgag tgcggataca agtgccgcgt
     6121 tgtctcctga aagttcgcgc gcgcgcatga gactctggag gtcagaacat ttgttgctct
     6181 ctgcttctcc ttccgcgagg tagccgcgca ctgtacttgc gatgtcagac agatagcgga
     6241 ttctaccagc ggggaggatt tcagttgtat cgagatccgg gctcgatcga tcaatgggag
     6301 caaaggaaac tccagttttt tttgcaatga ggtctacgag tgcggagtaa agctgtgtaa
     6361 ctccactgtc ctgaaagcgt gcagcaactg tagtgtacac tggcatttcc tgcggatctt
     6421 cagagaatcg attgtgattc ctttgatact gcttccttac atgcatcaga gcgtctttcg
     6481 cgccagctcg atctcctttg ttcagcgcca cgatgtccgc gaaatcgagc atcgcaattt
     6541 tctctagctg agaaggcgcg ccgaattcag gcgtcatcgc gtacagattt acgtccgcga
     6601 tttcagtgat ttccgcatct gattgcccgg aaccagaagt ctctacaatg atcaggtcga
     6661 aagggagtct cttgaaaacg gaaaggattc tgctgagagc atcgctgact gtaccacctg
     6721 attttctcgt cgcaagggag cggaagaata tctgatctgg atgaagcgaa agagaattga
     6781 ttcgaattct atctccgagc aatgctccgc cagttttctt tctggttgga tctactgcga
     6841 atacgcagat gcgaaggtga gggttggctt caaggaatcg gttgagaagc tcatctgtga
     6901 gagacgattt gccagcgcca cccgggccag taatcccaag gacaggacag ctcttttcgc
     6961 ccggctcgat cgggggattc tcaccttctt ctatcattgt aatgaggcga ccgagtacct
     7021 ccggctttgt actgtctccc tggatgatgc ccagatcttt tggtgagaaa tctgttcctt
     7081 cgagcatcga cgaaatgatt ccggcaaggc ccagagtgcg tccatcttca ggagaaaaga
     7141 tctttgtgat tccggaagat tccagatcct ggatttcttc ctggacaatg acgccgcctc
     7201 cgcctccgta aatcttgatg tgatctgctc cgcggctttt caataattgt ttaagatatt
     7261 taaagtattc aacgtggcct ccctgatagc tggagatggc aatgccctgt acgtcttcgc
     7321 acagagctgc ttccactaca tcctgcacag agcgattgtg ccccagatga atgacttcag
     7381 cgccgtagga ctgcagtagt ctgcggaaga tgttgatgga tgcgtcgtgc ccgtcgaaca
     7441 gggaagctgc tgttacaaac cgaatatgat gtttcggttg atagatttct ggatggtttg
     7501 gtttttctgc tgtcatggcc tgaagaattg atccatgagt actggatctg gtttgataaa
     7561 ttcgcctggt tcagaggcgc cgtttggatc gatgaatttc tgactgactc cgatgagact
     7621 tccacggaat gtaaggtcca ctctacaaat gcttctgccg gcaacaatgc acacgtaagg
     7681 tccggcagga tcgcgataga atagattttt ctttcctgat tcgatgacca ggaatcggcc
     7741 aagttcacct accgctgagt gcacgtttcc ggtctggccc gcgaagattg cttccgtctg
     7801 tggttcggag agaagcaaaa tcgaaaagtc tgcgtccgct ttgtgagcgc tggattcgag
     7861 atcgcgcaac agcgcgtctg atttcctgtc tttgagagga tccagtcccg tgatccacca
     7921 cttgagagtg gagtttgcag tgatgatttc cgttttgcat tttgcttttg gagtgcattc
     7981 gcgttctaca acgggcagat ccaggctcgt tttttcaagc agagctcttt cttgctttga
     8041 gaatgtaagc gcggagaatc ctacgtactg gatgagtcct ggatcgggtc ttttgagaga
     8101 atcttccagt gcagtgctga gagtggcctg tgtgaattgt cctgcgtgca ctgcgatgac
     8161 agatccgccg cgcggtgcga tcttctgttc aattcttctt ttgagtgcga ggaacgcggc
     8221 cagccctccg cgtcctttct ggtcgactgc gaagtctcct ttccagtccg cgatggcaag
     8281 tatcgtgacc gttcgctcag tttgcgcgga gagtgcggat gcaaacaaaa gcgccagcag
     8341 caatccgggt cctttcatgg ttcacagtca gttgttttgg acagggtgca agcaactttc
     8401 gaaacgtcag cgaagcctat ttcgtggtca tttgctctac gccattccag ccttcgggca
     8461 cgcccttctc catgtacaat ctgcatcgcc tcacgaagat tccggcggcg cgatcatcgg
     8521 ggttgctttt caatacttca atgaaaagag aacgagcgtt ttcaaatttc tggctgaggt
     8581 acaatttctg ggcttcatca aatttcgcgc gcgaagcttc ctttagctgg cggactgaat
     8641 ctgtttctcc gtcgagcact tcgattacaa tgaccggttc ctttttcccc ttcacctgca
     8701 cccggtccag agtgcgaaat gagaaacgag atggatcttt taaaagactt tgagtgcgat
     8761 tggaaatgat gagagacgtt ccatagattt ttgtcagccc ttccatgcgc gaagccaggt
     8821 tgacgtggtc actgatcacg gttccttcca tgcggctttt ttcaccaatt gtgccaagca
     8881 tcagatcccc ggtgtgaagg ccgattccag tgcgaacagg tgcgtaaccg gcactcaatc
     8941 ttccgtgatt ccagacttct agagcgcgaa gcatgccgat cgaagacata agagcgttgt
     9001 ccggagaatc gaacagcgcc atgatcgcat ctccgatgta cttgtcgatg aagccgtggt
     9061 gttcgcgcac caccggccca acctgattga gataactatt aataaatcta aagttttgtt
     9121 ccggtgtcat ggattcagac agagatgtga aggatctgat gtctgagaag agcaccgtca
     9181 tttctcgcgc gatgttgtct cccagagaga ctccgactat gttttctttg cccagtagtt
     9241 tcaggaattc agagggaaca aatcgactga ttgcttcatt gatttctact tgagcctgtc
     9301 tggcgagttt ctcctgctcg agtgctcttt ctagattgct atagagcttt gcattctcca
     9361 gggaagtagc gatctgagcc gatagaacct gaagtgcttc gagtctggcc ggactgaata
     9421 ctccaggcgc gccgttattt tccagataca gaatgcacga taatttcccc tgcaatacga
     9481 caggcatgca caggacactt cgcggtttgt attctagaat gtacggatcc gcgcgaaaac
     9541 gcgaatcgga ggcggcctca tctactacaa tgctggtgcg agtgcgttcc gctagaagaa
     9601 caatagaatg cggcagcagg ttgtaatctt ccagagggac attcggaaca ggcgtaacct
     9661 gatcggtgag cgcgtttcca gctgcggaaa ctacgaccgc gccctcctga tcgagaagaa
     9721 ggtgtgcttt ttgtgcacct gcgttttcca ttacgatccg gagaagtttt ccaagcagac
     9781 cttcgagctg gatctctccg gaaatggatg cggcggcttt tacaatcgtt tgaaaatcaa
     9841 aaccgcctgc ctgtgcaaga ccgcctgaac ctgacatttg cgatccgcgc acaagttcgg
     9901 ggaaatgatt ctcgatttcc tctactttgg ccgtgatgtc aagtgcgccg tagatggctc
     9961 ttgcttcgcg cacatacagc aatgcgaatt t
//
LOCUS       DAOQJL010000079         9850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3627_length_9850_cov_4.600102, whole genome shotgun sequence.
ACCESSION   DAOQJL010000079 DAOQJL010000000
VERSION     DAOQJL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3627_length_9850_cov_4.600102"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLG78_05285"
     CDS             <1..771
                     /locus_tag="PLG78_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE22555.1"
                     /translation="FFGLGVFFAGLAVTYWFFRKIPPAVFFESLGLIDPERQRLLLES
                     AVLYVFLVFPVGAAFGKQVAHLFPNATLFALVHLTIAFFASCGYYALNAGPGSLRMLA
                     VGQVTDPMKEVAVGVMGFAAIFPPALLALLLTMGSAAQDSVRIAHPISFEIQKNPVLV
                     FILAAVLVPIFEEVIFRCFLYGFFRREQRIRYAAFFSGSIFAILHPQGAIALPYLTIL
                     GMGLAVLREYRPSPLASMTAHACVNGTAVILGYAFTAL"
     gene            complement(768..1571)
                     /locus_tag="PLG78_05290"
     CDS             complement(768..1571)
                     /locus_tag="PLG78_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNE22556.1"
                     /translation="MTGVFVERIIVYPIKSLDGVSVSECRITDGGSLEHDRLLAIVDE
                     QGTFVNAKRFAKIHAIRAVYTEDFRAVTLSAPDKPAHQFSMEDRDRMSLWFSDFFEMN
                     LRIMQNTQTGFPDDLEASGPTFVASATLQRVCEWFAASGQGFKFEEAIRRFRPNVLFG
                     GADSFYEDQLYGGATFFAGDVTVVGSNPCARCAVPSRHPSTGEVIQGFSKTLSDARKK
                     HLPATSNPDHFSHYYRLCLNTRIHSSETGKTLRVGDAVQTEWDSWGKIF"
     gene            complement(1580..2245)
                     /locus_tag="PLG78_05295"
     CDS             complement(1580..2245)
                     /locus_tag="PLG78_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22557.1"
                     /translation="MAFSKPGSLPFAFCAVFVCVTVAADAKPRNQKQITSATPVNKES
                     DKPKQEPSPDQNRSEKPTQDEKKEKAYVVESRITLGDGRIVNGRLKFNAPEKITLVHE
                     AEGIQYEKVIGMGEISSIEILKWKGKFLRENKNGMVYEFAAEEFRIRLKDGHELRKSG
                     GLFPFLRKIPLENKNGQIQVFTFWMDLKKPDGTWYTGIAGPDTGLRNTCHPDVIRRID
                     FEK"
     gene            complement(2232..3308)
                     /locus_tag="PLG78_05300"
     CDS             complement(2232..3308)
                     /locus_tag="PLG78_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNE22558.1"
                     /translation="MALAGVYRTERKIQPIEAQRPSLSKDELESVLDCLIHDRLMSGS
                     VAGRFEKSFAHAFGFKHALSVNSQTAAYHLAYLALGVGAGDLVAMSSIAPVGAYDAAR
                     YTGADVALVDIERGSFHPSQDAVQAALSREAGKKIYILDHTFGAPASLEFPGENVQII
                     ADFTGLVGAEIQASQFGALSICGMSQYDLMTTGNGAIIVTNDSKLFQKIDSLRYGSKR
                     QEGSIAYDYRLEDFQAAIGLEQLTRLAVTLARRRKIGVKYIESLQRTQHETYFKTPGT
                     DCYLRFPIVVSRPHDEVIRYFNSLQIGVTRAVDLPLHHLAGLPRLEYPNAERLYQKSV
                     SVPVYPALSANNVERIAQSLRGLL"
     gene            complement(3452..4576)
                     /gene="manA"
                     /locus_tag="PLG78_05305"
     CDS             complement(3452..4576)
                     /gene="manA"
                     /locus_tag="PLG78_05305"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNE22559.1"
                     /translation="MSRRSHYLLKCPVQKYHWGSLDAESILRRIAFKAHEAVLEDEPA
                     AELWMGAHPTAPSIVQPENESLASLIATEPDYFLGHGGHLSFLFKILHADRPLSIQAH
                     PDRTLAKQLHARDAKNYPDPNHKPELAMCIQDMRALVGFRNENEIRVELERHAALLEI
                     CGHIEDGVRGWYAGLMRTDGEKVARAAERVRSAVSREPEEICFLDLCGIYGDRDPGIF
                     APFFLNYMELAHGEAIFLGPNEPHSYLGGEILECMAASDNVVRAGLTTKYCDVDTLVS
                     MLHYRTGPHQIVKPVQEANGLRYPVPVNDFALHKINQPIHRRGPAIVLALKGTMEING
                     EIFPSGSAVFIPHGQPSTLAPASGSEIYSCSTPEDFNPPD"
     gene            complement(4573..4776)
                     /locus_tag="PLG78_05310"
     CDS             complement(4573..4776)
                     /locus_tag="PLG78_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22560.1"
                     /translation="MQRFFTILKYDFLALVPGSTVPFSLHAMAKLILYAGFILFVFFL
                     FYRLPVRIHEPEQPRQPSIERPI"
     gene            4856..5833
                     /locus_tag="PLG78_05315"
     CDS             4856..5833
                     /locus_tag="PLG78_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22561.1"
                     /translation="MRFCILIGLLCFCGPLFSQPVADTSVDQVSGPDGRFRVLRIFPM
                     HYWLDSEQESNVRALTEASGPGDPLVVVLWGPYTPRVLDANAERSRIVPQHFEWFLHR
                     LCYKDVNYEDPAYRKHQMAESRCYAERTVEFLKGRTLPYFMFAMWGDNLESYEYIGLI
                     RYPVILLTMVVFSIPQLLLFLLILLPFKSWLSSREFVQRVFKVVLPMIIGIAWLFFFM
                     ERVGDEWYITPFRFVLPMSGVYFALTFISCMWIADYVYFRQNNPPSPWVVAFFYVFAP
                     IMILGAGAAARGSRGTYVSSRGSSSGTSGGSGSTGGGGSFGGGGASASF"
     gene            complement(5834..7573)
                     /locus_tag="PLG78_05320"
     CDS             complement(5834..7573)
                     /locus_tag="PLG78_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE22562.1"
                     /translation="MEWDQAIFKKVLTLAGRLKKKGTLNPNRSILSELKDRLTIVSRM
                     LTGDPVEIVTAEAGGGFRNQFFFLPAFYEGFESRADNDLFFFLRVCCLAEQRRMGLNW
                     SKDGQTEEESISRATGSLEAILSAVAKKYPSLRLTIDSVIRTELATNGSLKLLAGKWM
                     APIESSGGEVTPGSRNTQSQKEQKEKVTEIDGKTREDVRTHTPDTKAIEDYTLSHNFE
                     KAETIEEFNGTWRSMDGEDDLSDHEEALKELDLRDTVRVDTPAHSVYRTEFFVNASAP
                     ESASSTEEGTFIPYDEWDYKIRSYKKAHCRIYPGRPRNLSADYVRSALDQDRGTLARL
                     RRTMSSVNNEMEQVRRQSSGEEPDLDALVDNFSELAAGKTPSEKVYLSRRKRKRDLAV
                     LILLDISLSTDAYTGGERILDVEKRSVALLSEILAESEDDFAIDAFFSKTRNHISYLN
                     VKRFSEGWAPARGRLGGLEATGYTRIGPAIRHATAEILSRSARKKWILLLSDGKPNDY
                     DRYEGRYGMADVRRALKEARNADVHTFALAVEASARHYLPLMLGQGRYRILPHPSMLP
                     EALTEFYARLRAG"
     gene            complement(7594..8391)
                     /locus_tag="PLG78_05325"
     CDS             complement(7594..8391)
                     /locus_tag="PLG78_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNE22563.1"
                     /translation="MPQNADDANGLPYYKPVGRECEVFEHAYKNRLPLLLKGPTGSGK
                     SRFVEYMSAKMKLPMITVSCHDETSAVDLLGRYLVKGSETTWQDGPMTRACRTGAVLY
                     LDEIAEARPDTIVALHSLTDHRRILYLDRHDEEIAAPPTFMLIASFNPGYQKGWKELK
                     ASTRQRFVSIEFQYPSEAVESEIVRAESNLDLSQVKLLVQLAGRIRNLAELGLAETVS
                     TRLLVSTAKLMADGLPARLACDVGIVQPLTDDAETGAAVRDVVALVF"
     gene            complement(8372..9745)
                     /locus_tag="PLG78_05330"
     CDS             complement(8372..9745)
                     /locus_tag="PLG78_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE22564.1"
                     /translation="MKYKSQKIAYWFFATCMLLFSLQIIYGFVMGFARIGMDGLHDFI
                     PFNAARATHTNLLVVWLLTGFMGAAYFIIPDESGRELFSVKLAWVQLISLIVVGVVAI
                     IGFHLNYWEGRKFLEIPRPLDFLVVVNVLMFIFNIGMTILKGNRYSTTQLVLFIGLLS
                     AALLYLPGMIYFRSQTMDSYFRWWVVHLWVEGVWELIMGSILSYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGLLGTGHHYYYIGTPKYWLMVGGIFSALEPLAFLGMALFAINMYR
                     KSGREHPNKISLYWTIGCSIMSFVGAGFLGIAHTIPQVNLWTHGTLVTAMHGHMAFWG
                     AYAMIVLAVITYCLPLLTGRKLWNNLPGLLSFWLANIGMTAMTGAFAVAGITQVYLER
                     KMGMDFLTVQKEIQIHFVGLIAAASLFTIGAILFIYNFIKFGLPSDEAIVPENTSDEA
                     LYAAKRG"
BASE COUNT         2192 a         2466 c         2523 g         2669 t
ORIGIN      
        1 ttttttgggc tgggcgtatt ctttgcgggt ctggccgtca cctactggtt ctttagaaaa
       61 atcccccccg ctgtgttttt tgaatccctc ggactcatcg atcctgaaag gcaacgtctc
      121 cttctggaat cagctgtatt gtatgttttt cttgttttcc cggtgggagc ggcttttggc
      181 aagcaggtag cgcatttgtt cccgaacgcg acgttatttg ctcttgttca tctcacgatt
      241 gctttctttg cttcttgcgg atactatgca ctcaacgcgg ggccagggtc cctgcgtatg
      301 ctcgcggtcg ggcaggtcac cgatccaatg aaagaagtgg ccgtgggagt catggggttt
      361 gctgccattt ttcctcctgc attgcttgct cttcttctga ccatgggatc tgcggcccag
      421 gattccgtgc gaatcgctca tccgatttct tttgaaatcc agaagaatcc ggttcttgtt
      481 tttattcttg cggcagtcct tgttccgatc ttcgaagaag tgatctttcg ctgttttctc
      541 tacgggttct tcagacgaga gcagcgcatt cgctatgctg catttttttc agggagcatt
      601 tttgcgattc ttcatcctca gggtgccatc gcactgccat acctgacgat tctgggtatg
      661 ggactggcag tactgcgaga gtatcggcct tctcctctcg catccatgac tgcacacgca
      721 tgcgtcaatg gcacggctgt aatactggga tacgctttca cggctctcta gaagattttg
      781 ccccaggaat cccattcagt ctgcaccgcg tctcctacgc ggagagtctt cccggtttct
      841 gacgaatgga ttctcgtatt caaacacaat cgatagtagt gcgaaaaatg atccgggttt
      901 gaagtggccg gtaagtgttt ctttctggcg tcactgagag tttttgagaa accctgaatg
      961 acttctcccg tagatggatg tcgtgacggc acggcacacc gggcgcacgg attgcttccg
     1021 accacggtta catctcctgc gaaaaaagtg gcacctccgt agagctgatc ttcgtagaaa
     1081 gagtctgcgc ctccgaaaag aacattgggt ctaaagcggc ggatcgcttc ttcgaatttg
     1141 aatccctgac cgcttgcagc aaaccattcg catacccgct gcaaagtggc agaagcaaca
     1201 aacgtgggac cagaggcctc tagatcgtct ggaaaaccgg tctgagtgtt ttgcatgatg
     1261 cgaaggttca tttcgaagaa atcactgaac cagagagaca ttctgtctct gtcttccatt
     1321 gaaaactgat gcgctggttt gtcgggcgcg cttagagtta ctgctcgaaa atcttctgtg
     1381 tagacggcgc gaatcgcgtg gatcttcgcg aagcgttttg catttacgaa cgttccctgt
     1441 tcgtctacga tcgccagaag tctatcatgt tcgaggctac ccccgtccgt tatgcggcat
     1501 tcggagacag atactccgtc cagtgacttg attgggtaga cgatgatcct ttcaacaaag
     1561 acgcccgtca tgcctgttgt tacttctcaa agtcgatgcg acgaatgaca tccggatggc
     1621 aagtgttgcg aaggcctgtg tccggtcccg caatgccagt ataccacgtg ccgtctggtt
     1681 tcttgagatc catccagaac gtaaagacct gaatctggcc attcttgttt tctagtggaa
     1741 tttttctcag gaatggaaac agtccgcctg atttgcgaag ctcatggcca tctttgaggc
     1801 gaatgcgaaa ttcttctgcg gcaaattcgt agaccattcc gtttttgttt tcgcgcagaa
     1861 atttcccttt ccatttgagt atttcaatcg aggaaatctc acccattcct atcactttct
     1921 cgtactggat gccttctgct tcgtgaacca gggtgatctt ttctggagcg ttgaatttga
     1981 gtcgtccatt tacgattctg ccatctccca gagtgattct ggattcaact acgtaggctt
     2041 tctctttctt ttcgtcctgg gtcggctttt cgcttctgtt ttgatccgga gatggctctt
     2101 gctttggctt gtccgattcc ttattcacgg gagtcgcgga cgttatttgc ttttgattgc
     2161 gaggctttgc gtcagcggca accgtgacgc atacaaaaac cgcacagaat gcgaagggca
     2221 gagaacctgg cttagagaag gccacgtagg gattgcgcaa ttcgttccac attgtttgcc
     2281 gacagtgcag ggtaaaccgg cacagaaact gatttttgat acaatcgctc tgcatttgga
     2341 tattcaagac gcggcaagcc tgccaggtga tgaagtggga ggtccactgc gcgagtcaca
     2401 ccaatctgca atgagttgaa gtaacgaatc acttcatcgt gcggtctgga aacaacaatt
     2461 ggaaagcgaa ggtagcagtc tgttcccgga gttttgaaat aggtctcgtg ttgagttctc
     2521 tgcagagatt caatgtattt aacgccgatc ttgcgtctgc gcgccagtgt cactgcaagc
     2581 ctggtcaatt gttccagacc aatggctgct tgaaaatctt ccagcctgta atcatatgcg
     2641 atggatcctt cctggcgttt ggatccgtag cgaagagaat ctattttctg aaaaagtttt
     2701 gagtcgtttg ttacgatgat cgcaccgttt ccagtcgtca tgagatcgta ttgagacatg
     2761 ccacagatgg acaacgctcc gaattgagat gcctggatct cggcccccac aaggccagtg
     2821 aagtcagcga tgatctgaac gttttcgcct ggaaattcca gagatgcagg agcaccgaat
     2881 gtatgatcca gaatatagat tttctttcca gcttcgcgcg aaagtgcagc ctgcaccgcg
     2941 tcctgcgatg ggtgaaagga gccgcgctcg atgtcgacaa gtgcaacgtc tgctcctgta
     3001 taccgggctg catcgtaagc tccgactgga gcaatcgatg acatggcaac caggtccccg
     3061 gcaccgacgc ccagtgccag ataagccagg tggtaggctg cggtctgtga gttcacgctc
     3121 agcgcgtgct taaaaccaaa tgcatgtgcg aatgactttt caaaacgacc ggccacgctg
     3181 ccgctcatca gtctgtcatg aatcaaacaa tcaaggacgg attccagttc gtccttgctc
     3241 aggctcggtc tttgagcctc tatcggttga atttttcttt ccgtcctgta taccccagcc
     3301 aaagccacaa ttctagtcct gtttatattt cgtcctttgt aagatattat gtcacattgc
     3361 gtccagccct ttttagcttt tgcggcaaaa tttagaactc cctgatttga cgttccgccc
     3421 gggtccgggc ggtcagttgg ttttcaaaaa ttcagtctgg aggattgaaa tcttcaggtg
     3481 tagagcagga gtaaatttcg gaaccggaag cgggtgcaag cgtagaaggc tgcccgtgcg
     3541 ggatgaacac cgcgcttccg gatggaaaga tttcgccgtt tatttccata gtacctttca
     3601 atgccagcac aatggcgggg ccgcgtctat ggattggttg gtttatttta tgaagcgcga
     3661 aatcgttcac tggcacggga tatctgagcc cgtttgcttc ctgaactggt tttacaatct
     3721 ggtgcgggcc cgtgcgataa tgcagcatgg atacaagagt atccacgtcg cagtattttg
     3781 tagtgagtcc ggcgcgcact acattgtctg acgcagccat gcactcgagt atttctcccc
     3841 ccagatagga gtgaggttca ttggggccaa ggaagatagc ttccccgtgc gccagttcca
     3901 tgtaatttag aaagaaaggt gcaaatattc cagggtcgcg atctccatag atgccgcata
     3961 gatccagaaa gcagatttct tccggttccc gggaaaccgc agagcgaact ctctccgccg
     4021 cacgagccac tttttctccg tctgtgcgca tcaatcctgc gtaccagccg cgcacaccat
     4081 cttcaatatg tccgcatatc tcgagcaatg ccgcatgacg ttccagttca acgcgaattt
     4141 cgttttcatt tctaaatcca acaagcgcgc gcatgtcttg aatgcacatg gcaagttctg
     4201 gtttgtgatt cgggtccgga tagttctttg catctcttgc gtgaagttgt tttgccagtg
     4261 tgcgatccgg atgggcctga atggaaagtg ggcgatctgc gtgcaagatt ttgaaaagaa
     4321 agctcaggtg tccgccgtgg cccaggaaat agtcaggctc agtagcgatc agagatgcca
     4381 ggctttcatt ttcaggttga acgatggacg gcgcagtagg atgggcgccc atccacaact
     4441 cagccgctgg ttcgtcttcg agtactgcct catgagcctt gaaggcgatg cggcgcagga
     4501 tgctttccgc atccagggag ccccagtggt atttttgaac gggacacttc agtagataat
     4561 ggctgcgtcg actcaaatag gcctctctat ggaaggctgg cgcggctgct cgggctcatg
     4621 gatccgaaca gggaggcgat agaaaagaaa gaacacgaac aatatgaatc cagcgtacag
     4681 aatcaatttt gccatcgcat gaagcgaaaa ggggactgtg ctgcccggaa cgagggccag
     4741 aaaatcatat ttcagaattg taaagaatct ctgcacgatt ccagcgtgta aggcgtgttt
     4801 ttgcggtcaa acttattcga gttgaaatcc tatacggcga gagagagaac tgctgatgcg
     4861 tttttgcatt ttgatcggtc tactttgctt ttgtggcccg ctcttttcgc agccagtggc
     4921 agacacttct gtggaccagg tttcggggcc agacggcaga tttcgcgtgc ttcgcatttt
     4981 cccgatgcac tactggcttg attcagagca ggagtcaaac gtgcgcgctc tgacagaagc
     5041 atcagggccc ggcgatcctc ttgtggttgt gttgtggggg ccctatacgc ccagggtgct
     5101 cgatgcaaac gctgagagat ccaggatcgt tccgcagcat tttgaatggt tcttgcatcg
     5161 gctgtgttac aaagacgtga actacgaaga tccggcttat cgcaagcatc aaatggcaga
     5221 aagtcgctgt tatgcagagc gcaccgttga gtttttaaaa ggcaggactc tgccgtactt
     5281 catgtttgca atgtggggcg ataatctgga gtcctacgaa tacatcggtc tgattcgcta
     5341 tccagtcatt cttcttacta tggtcgtgtt ctctattcca caattgttgc ttttcctcct
     5401 gatccttcta ccattcaaat cgtggctttc gagtcgtgaa ttcgtgcagc gagtctttaa
     5461 ggtagtgctc cccatgatca ttggaattgc ctggttgttt tttttcatgg agagagtggg
     5521 agacgagtgg tatatcacgc cgtttcgttt tgtgcttcct atgtctggag tttattttgc
     5581 cctcactttc atttcgtgca tgtggatcgc agactacgtt tactttcgcc agaacaatcc
     5641 gccatccccc tgggtagttg cattttttta tgtttttgct cccatcatga ttcttggagc
     5701 gggtgcggcc gcccgtggtt ccaggggaac atacgtctct tctaggggtt ccagttccgg
     5761 gacgtctggc ggttccggct ccaccggcgg aggcggaagc tttggtggag gaggagcgag
     5821 tgcgagcttt tagttagccg gcgcgcaatc gtgcgtagaa ttcagtgagc gcttccggga
     5881 gcatggaggg atgaggcagg attctgtatc ttccctggcc cagcatcagt ggtaagtaat
     5941 gtcgcgcgga tgcttctacg gccagcgcga atgtatgaac atccgcattc cgcgcttcct
     6001 tgagcgctcg tctgacatct gccattccgt agcgtccttc ataccggtca tagtcgtttg
     6061 gtttcccatc agatagaagc agaatccatt ttttccgagc gcttcgcgag aggatttcag
     6121 ccgtggcatg acgaatcgcc ggaccaatgc gcgtgtatcc tgttgcttca agcccgccga
     6181 gtcttccgcg cgcaggagcc cacccttcgg aaaagcgctt aacgttgaga taggagatat
     6241 gatttcgcgt tttagagaag aacgcatcga ttgcaaaatc atcttcagac tctgcaagga
     6301 tttctgaaag cagcgccacg gaccgttttt ccacatcgag tattctttct ccgcctgtgt
     6361 atgcgtccgt ggacaggcta atgtccagaa gtatcaagac cgcgagatct ctttttcttt
     6421 tgcgccggga caggtagact ttttcggatg gtgtttttcc ggccgcgagt tctgaaaaat
     6481 tgtccaccag tgcgtccagg tcgggctcct ctcccgaaga ctgtctgcga acctgttcca
     6541 tttcattgtt tacggatgac atggttcgcc tcaggcgagc cagggttccg cggtcttgat
     6601 ccagtgcgga tcgcacgtaa tctgcggaca ggtttcgcgg acgacccgga taaattctgc
     6661 agtgtgcttt tttgtaggat cttattttgt aatcccattc gtcgtaggga ataaaggtgc
     6721 cttcctctgt agagcttgca ctttctggcg cggaggcatt tacaaagaac tcggttctgt
     6781 aaacggaatg tgccggtgtg tccactcgaa cggtgtcgcg caaatccagt tccttgagag
     6841 cttcctcgtg gtcgctcaga tcgtcttccc cgtccatgct tctccatgtt ccgttgaatt
     6901 cctcgatggt ttccgctttc tcgaagttat gcgatagtgt gtagtcttca attgcctttg
     6961 tgtccggagt gtgcgtgcga acgtcctctc ttgtcttgcc atcaatctcc gtaacctttt
     7021 ccttttgttc tttctgcgat tgtgtgttcc gggaacccgg cgtcacttct ccgccgctag
     7081 attcaatcgg ggccatccat tttccagcca gcaacttcag gctaccgttt gtggcaagct
     7141 ccgtgcgaat cacagaatca atggtgagac gcaaagatgg atatttcttc gccaccgcgg
     7201 aaagaattgc ttcgagtgag cctgttgcgc gcgatatgga ttcttcctct gtttgtccat
     7261 ctttcgacca gttgagtccc attctccttt gctctgcaag acagcatact ctgaggaaga
     7321 aaaagagatc attgtctgcg cgagattcaa atccttcgta gaatgcgggc aaaaagaaga
     7381 actgattcct gaatcctccg cctgcctccg cagtcacgat ttccacggga tccccggtga
     7441 gcattcgtga aacaatcgtt aggcggtctt tgagctctga caggatggag cgattgggat
     7501 tgagtgttcc tttctttttg aggcggccgg cgagcgtcag caccttcttg aagattgcct
     7561 gatcccactc cattgtaatc tctggaagcg ccgttaaaac accagggcta caacgtctcg
     7621 gacggcggcg ccagtttctg cgtcatctgt gagcggttgc acgattccta cgtcgcaggc
     7681 gagtctagcc ggaagtccgt ctgccatgag ctttgccgtg ctcaccagca atctagtgga
     7741 tacagtttct gccagtccga gttccgccag atttctgatt ctacctgcca gctgcaccag
     7801 cagcttgacc tgagataaat ccagattgga ttctgcgcga acgatttcag attccaccgc
     7861 ttccgagggg tattgaaact cgatcgatac gaagcgctgt cgcgtggatg ctttgagttc
     7921 tttccatccc ttttgatagc caggattgaa agaggcgatc aacatgaaag tagggggtgc
     7981 tgcaatttct tcatcatgtc tgtccagata gagaatgcgt ctgtggtccg tgagtgagtg
     8041 aagtgcaacg atggtatccg gtcttgcttc cgcaatttca tccaggtata gaaccgcgcc
     8101 agttctacac gcgcgcgtca tgggtccgtc ctgccaggtt gtttcagatc ctttgaccag
     8161 ataacgtccg agtaaatcca ccgcagaagt ttcgtcgtgg caggaaactg taatcatggg
     8221 cagtttcatt ttcgcggaca tgtactccac gaatctagac ttgcccgaac ccgtgggccc
     8281 cttcaaaaga aggggcagac ggttcttgta agcgtgttca aaaacttcgc attcgcggcc
     8341 taccggttta tagtacggca ggccgttggc gtcatccgcg ttttgcggca tagagggctt
     8401 cgtcagatgt attctccggt acaattgctt catccgacgg caggccgaat ttgatgaagt
     8461 tgtagataaa gagaatcgcg cctatggtaa acagactcgc ggctgcgatc aatccaacga
     8521 agtgaatctg gatttccttt tgaactgtaa ggaagtccat tcccatttta cgttcgagat
     8581 acacctgcgt aattccagcg acggcgaacg cgcctgtcat cgcagtcata ccaatgtttg
     8641 caagccagaa tgaaagaagg cccggcagat tgttccagag ctttcttcca gtgagaagcg
     8701 gcaaacagta cgtgatcacc gcaagaacaa tcatcgcgta ggctccccag aatgccatat
     8761 ggccatgcat cgccgtaacc agcgtgccgt gggtccacag gttcacctgc gggatcgtat
     8821 gcgcaattcc caaaaatccc gcaccgacaa aagacatgat ggagcaaccg attgtccaat
     8881 agagtgaaat cttatttgga tgttctcgac cgctcttgcg atacatgttg atggcaaaga
     8941 gcgccatccc aaggaaagcg agtggttcga gagctgagaa gattcctcct accatcagcc
     9001 aatatttagg agtgccaatg tagtagtagt gatggcctgt tcccagtagt cccgaaagga
     9061 acgtgagtcc tacgatcaca tacaaccact tctcaatgac ttctctgtct acgcccgtca
     9121 gtttgatgag caggtaagaa aggatggatc ccatgatcaa ttcccagaca ccttctaccc
     9181 acagatgaac gacccaccag cggaaataag aatccattgt ctgactgcga aagtagatca
     9241 tccctggaag atacaacagc gccgcagaaa gaagtccaat gaacagaacc agctgcgttg
     9301 tcgaataacg gttccctttc aggatggtca tgccaatgtt aaagatgaac atgagcacat
     9361 tgaccaccac gagaaaatcg agcggtcttg ggatttccag aaacttgcgg ccttcccagt
     9421 agttcaggtg gaatccaatg atcgccacca cacccactac tatcaaagag atgagctgaa
     9481 cccacgcgag tttgactgaa aatagttcgc ggccggattc atctggaatg atgaagtagg
     9541 ctgctcccat aaaaccggtg agaagccaga ccaccaggag gtttgtatgc gttgctcttg
     9601 ctgcgttaaa cggaatgaag tcgtgcagtc cgtccatacc aatgcgcgcg aagcccatta
     9661 cgaaaccgta aataatctgg agtgaaaaca gaagcatgca ggttgcgaag aaccagtatg
     9721 caattttttg cgatttatat ttcatctaga tcctcccgac ctacttcaat cccgcaagaa
     9781 atgtggagat ttctttgatt tcggcttcgc tgagtgcgag tttgggcatt ttcgctccgg
     9841 gctttattgc
//
LOCUS       DAOQJL010000080         9835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3636_length_9835_cov_5.108078, whole genome shotgun sequence.
ACCESSION   DAOQJL010000080 DAOQJL010000000
VERSION     DAOQJL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_9835_cov_5.108078"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..901
                     /locus_tag="PLG78_05335"
     CDS             131..901
                     /locus_tag="PLG78_05335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNE22565.1"
                     /translation="MSQISTQKRLKKLEVKIAEDQLEIEKALRLRYEVFNQEMGEGLP
                     QSASTGKDRDEYDLFCDHLIVVDHANENHVVGTYRLLTKTRALKNIGFYSAQEFDLSN
                     IYKLDFEIAEVGRSCVHPDYRDGSVITLLWAGIANYIQTHNIRVLMGCGSLHSTDAEE
                     VNMVYAFLRDEGHLVEEHLRVRPLPTSVHPGFREIELAGDRKSITKRLPPLIKGYMRI
                     GARIGGEPALDREFGTTDLFIYFDAESITNKYGNKFLK"
     gene            complement(902..1867)
                     /locus_tag="PLG78_05340"
     CDS             complement(902..1867)
                     /locus_tag="PLG78_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNE22566.1"
                     /translation="MSVLRGLRRLNRYEARLFRIARKAGKLAQIEQLGYSARSSDGFR
                     FPIYSLTIGKPAALERSPVGLVAGVHGLETIGIRVLLDFLESIFDEPNRDVSQALLDG
                     DIGIVSIPILNPGGVALKSRANPAGVDLMRNSGKDGDTALPFFGGHRMSSKLPYFRGK
                     SLQPESRALFRFCLERFSQARKGTILPVLDVHSGFGMKDSVWWPYAGKKTPSADHYLF
                     ERLSRILQTEGDGITYEPQSQAYTVHGDLWDRLYDDYRRSKKPAKLLPFTLEIGTWSD
                     VKRNPGRLLSKRGLFNPPPEQKALMIQRYRPFLSKFVLLSLLYGE"
     gene            complement(1864..3060)
                     /locus_tag="PLG78_05345"
     CDS             complement(1864..3060)
                     /locus_tag="PLG78_05345"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNE22567.1"
                     /translation="MNKLTVKDLNVAGKKVLVRVDFNVPLKNGVVENDKRLRASVPTV
                     QYLISNGARVILMSHLGRPKGKPVDSMRLGPVADAFGKLLGKPVKSIGTCVGDEAQAA
                     ASALKDGEVLLLENLRFHPEEEGNDPGFAKQLASLGDVYVNDAFGTAHRAHASTEGIT
                     HHVKQSASGFLLQKELDYLGGAVNNPKRPFVAILGGSKVSGKIDVIEALLPKVDFLLI
                     GGGMAYTFYKAQGLEIGTSLCEEDRIDMAKTLLAKAGTKLLLPVDCVISDAFDFDARK
                     VGTLKTVAANGIPKGWSGLDAGPETISLFKDKVLSAGTVLWNGPLGVFEIAETAVGTF
                     KIAEALAEATKKGAITIIGGGDSAAAIEKAGLEESVSHVSTGGGASLEFLEGKDLPGV
                     SALTNR"
     gene            complement(3089..4087)
                     /gene="gap"
                     /locus_tag="PLG78_05350"
     CDS             complement(3089..4087)
                     /gene="gap"
                     /locus_tag="PLG78_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNE22568.1"
                     /translation="MLKIGINGFGRIGRLAFRTALERQDIEIVGINDLLDADYMAYML
                     KYDSTHRAFKGTVEAKDGQLIVNGKKVRVTAEKDPANLKWGDIGAEVVVESTGLFTTI
                     EKAQAHIKAGAKRVVISAPSADAPMFVMGVNHKTYKADMTIVSNASCTTNCLAPLAKV
                     VNDRWGILEGLMTTVHAVTATQKTVDGPSHKDWRGGRGAYQNIIPSSTGAAKAVGKVI
                     PDLNGKLTGMSFRVPVADVSVVDLTCRLSKPAKYDDIKKAMKEASEGELKGILGYTED
                     EVVSQDFLGDPRTSIFDAGAGIQLSDTFVKLVSWYDNEWGYSNKLVDLIQHISTVK"
     gene            complement(4148..5476)
                     /gene="mnmE"
                     /locus_tag="PLG78_05355"
     CDS             complement(4148..5476)
                     /gene="mnmE"
                     /locus_tag="PLG78_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNE22569.1"
                     /translation="MTIAAIATYPGHAALGTIRVSGPGTLDIISRFVVAPDLKPIPFS
                     NQPRHSFFAKFMDANVLVDEVVAVYFKGPHSYTGEDSAEITFHGNPILLKSALRLLVE
                     SGIRLAEPGEFTSRAFRNGRLDLTRAESVKRLIEAKSEYELSAARAIYGGELSRRMSI
                     LRSAMIGLKAETEAEVDFSTEDLTFQSREERRARVMGLIAELDSLLARGSATGRIREG
                     ISIAIVGVPNAGKSSLLNRLLGYDRAIVTEVAGTTRDTLSEELQIAGIPVRLVDTAGI
                     RETNDIVEREGVRRSQRELEESTLVIHVIDGSRESYASIVESDRDNVIHVINKADRLH
                     PGANPGSAIQVSCLTGAGMDHVEEAIRDRVLERPESRDPILLEERHRFHLLAMKATLT
                     KIMELWQQNAPDEITALEIDDVIDHIGSITGRISNEEILGRIFSTFCVGK"
     gene            complement(5473..6237)
                     /gene="jag"
                     /locus_tag="PLG78_05360"
     CDS             complement(5473..6237)
                     /gene="jag"
                     /locus_tag="PLG78_05360"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNE22570.1"
                     /translation="MNYIYEAEGKTRAEAEQIALSALGLKEEDVTFEAVSAGKSFLGI
                     VNRKPTVVRLFAKNVPIETVIKGTVITLIRKMGLTVENVGVHIEEESFVVSIDSEDSA
                     ILIGKQGRTLDSLQFLTNCMVDSRIRNNRRVVLDVAEYRERRKQRLTKLAKAVADRVA
                     RTSQSVLLEWMNPYERRIVHLALEPDERVTTRSDGNGVYKRVRVLPQKGRPGSGPQDR
                     QDRPDRQDRERNFNRAPREEEEEDQDFNRKDAFDDQ"
     gene            complement(6258..8165)
                     /gene="yidC"
                     /locus_tag="PLG78_05365"
     CDS             complement(6258..8165)
                     /gene="yidC"
                     /locus_tag="PLG78_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNE22571.1"
                     /translation="MENKEEQKPGLGSFLPVLLIGIAVLLFFQFSGSGSHPPVTQEHE
                     GTGAKLTDFEFQSGPGEIVSVDTGNFIVKLNSQGGRIDKFYVKKSKELNVPDSVLKTE
                     TPEAQSYQAFEGSRGNGFDFQFHGYYYGDGASQLGDPALNKALFKKEGPFQDKDGVVE
                     VRFRLPVRLRGQNLEIVKIYRFLKGEHFFRQIAVIRNLDKKEFVWGGPMFFRPFGDVG
                     PSTEMAGGINPALHGRFYYYNGEFKSRMNTPPTNSILAPIGCGENLKDKPYSVYAEHP
                     NSLHFVGAHSKYFFAYAKFLDTNNPQSQVDGVAIRNEIDPDGKRAFTTILNDFRLAPN
                     TGELELQPAGAQTTGGHEYIKGIQQRNDAVILDQEIYYGVRTDESHAFANEALARTEF
                     GSSDTDKEARGVIYTSSFLALFSKVRDAIVWVMRFLHVYLGNYGWVIIVIAVSFKLIT
                     FPLNQMQAKSMKRMSALKPELEKIQEKYADNPAEKQKRTMEVYKKHNVNPAKGCVPIV
                     IQIPVFIALYSAFSESIELWRSPFILWMTDLSAPDTIYTIKDFLILKDFHINVLPLFM
                     VASQLLQQRLTTVITDPQQKIMMYMMPVIMIFFFWTMPSGVTLYWTVQNVLAIVWQLV
                     TNRMSDDGKTA"
     gene            complement(8171..8410)
                     /gene="yidD"
                     /locus_tag="PLG78_05370"
     CDS             complement(8171..8410)
                     /gene="yidD"
                     /locus_tag="PLG78_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE22572.1"
                     /translation="MNANTLAIAGIRVYQNTISKLIPGRCRFYPSCSSYAVECFERFS
                     FGHAFIKSGYRILRCNPFNEGYFDPVIPDEPQNQA"
     gene            complement(8746..8901)
                     /gene="rpmH"
                     /locus_tag="PLG78_05375"
     CDS             complement(8746..8901)
                     /gene="rpmH"
                     /locus_tag="PLG78_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNE22573.1"
                     /translation="MKRTFQPSNKRKNKTHGFRSRMATANGRKVLSNRRKKGRKRLTV
                     SDQTHRK"
     gene            9095..>9835
                     /locus_tag="PLG78_05380"
     CDS             9095..>9835
                     /locus_tag="PLG78_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNE22574.1"
                     /translation="MIKDAWNQVLKDFAGESSQNSSLKWLYSAKPGTSTDSSRLILNV
                     PDNGILSHLREYTRPIEVKMASILGRPVRVEFQLSHSLPEWIHPEYSFETFLESKCNS
                     NALQCIRAACRQPGRHNPIFLSGPANSGKTHLLHSYARSVIEAKPGARIAYLSGIAFK
                     LGHLRAIASRSSLDFKESMMSFDCVIFDDIHLIGSGKSTLEEIDFLITAFLDRGKQIV
                     CSSSTEPGSVFPSGRLRSRILSGIVVQME"
BASE COUNT         2466 a         2519 c         2249 g         2601 t
ORIGIN      
        1 taagaattat ccgcactgga tcgagcctcc gcccgatcca ggatgcattc actgaattgc
       61 atcccggcaa gagtttgaca cacgttcgtc atgctctaac gttagtctct ctccagaagc
      121 aggagagaac atgtcacaaa tcagcactca aaaacggctt aagaaattgg aagtcaagat
      181 cgcagaagat cagctggaaa tcgaaaaagc acttcgactg cggtatgaag tgtttaatca
      241 ggaaatggga gaaggtctac cgcagtctgc atccacaggc aaagacagag atgaatacga
      301 tctattctgc gatcatctga tcgtcgtgga tcatgcaaat gaaaaccacg ttgttggaac
      361 ttacagacta cttacaaaaa ctcgtgcact caagaacatt ggattttatt cagcacagga
      421 atttgatctt tccaacatat acaaattgga ttttgaaatt gcagaagttg gaagaagctg
      481 cgtgcatcca gattacagag acggaagcgt catcacactc ctgtgggcag gaattgcaaa
      541 ctacatccag acccacaaca ttcgtgtttt gatgggttgc ggctcgctcc attctacgga
      601 cgcggaagaa gtcaacatgg tgtatgcgtt tctccgcgat gaaggtcatc ttgtagagga
      661 gcatctgcgc gttcgccctc tccccaccag cgtgcatcct ggctttagag aaattgaact
      721 tgccggagat cgtaagagca tcacgaaacg cttgccacct ttgatcaaag gctatatgag
      781 aattggtgcg cgcattggcg gcgaacccgc gctggaccgt gaatttggaa cgacagatct
      841 attcatctac tttgatgcag agtcgatcac caataagtac ggaaacaaat tcctgaaata
      901 gtcattcgcc atagagcaag gaaagaagga cgaacttact caggaaagga cgatatctct
      961 ggatcatcag agccttttgt tcaggtggtg gattgaacaa tcctctttta ctgagaagcc
     1021 gccccggatt ccgcttaacg tctgaccagg ttccaatttc aagagtaaat ggcagaagtt
     1081 tggcgggttt cttgctacga cgatagtcat cgtacaggcg atcccagaga tcgccgtgta
     1141 cggtgtaagc ctggctctgc ggctcgtacg taattccgtc gccttctgtc tgtaaaatcc
     1201 tggacagtct ttcaaacagg taatgatccg cagacggcgt cttctttcca gcatagggcc
     1261 accacacgct atccttcatc ccaaatccag aatggacatc cagcacaggt agaatggttc
     1321 ctttacgcgc ctgagaaaaa cgctccaaac aaaaacgaaa gagtgcgcga gattctggct
     1381 gaagactttt gccacgaaag tatggcaact tcgaactcat gcgatgaccg ccgaagaatg
     1441 ggagcgctgt gtccccgtct tttcccgaat ttcgcatcag gtccacgccc gcaggatttg
     1501 ctcgactttt caatgcaact ccaccaggat ttaagatggg aatgctaaca atccctatat
     1561 ccccatcgag caaagcctgc gatacatcgc gattgggctc atcaaatatg ctttctagaa
     1621 aatcaagaag gacgcgaatc ccgatcgttt ccagaccgtg aacgcccgca accagtccaa
     1681 ccggagatct ttccagggca gccggttttc caattgttaa agaatatata ggaaatcgaa
     1741 atccgtccga gcttctggca gaatagccta gctgctctat ttgcgcgagc tttcctgctt
     1801 tgcgtgcaat tcgaaacaac ctggcctcat accgattgag gcgtctgagt ccgcgaagaa
     1861 cgctcaacga ttggtcaggg cactcacacc gggcaggtct ttaccttcca ggaactcgag
     1921 agaagcgccg ccacctgtag aaacatgact cacactttct tccagtccgg ccttttcaat
     1981 cgctgcagcg gagtctccac caccgatgat cgtaatggca cccttcttcg ttgcctcagc
     2041 cagagcttct gcaattttaa aagttccgac cgctgtttct gcaatttcaa aaacaccaag
     2101 gggaccattc cagagaacag ttccggcaga aagtactttg tctttaaaga gagaaatggt
     2161 ttcggggcct gcgtccagac cactccatcc ttttgggatc ccatttgcag caacagtctt
     2221 cagggttccc actttgcgcg catcaaaatc aaatgcgtct gaaatcacac agtccaccgg
     2281 caatagaagt tttgtcccgg ccttggcaag aagcgttttg gccatatcga ttctatcttc
     2341 ttcgcataag gaagttccga tttcaagacc ctgggccttg taaaaggtat aggccattcc
     2401 tccgccaatc aaaagaaaat ccactttggg cagaagcgct tctatcacgt ctatctttcc
     2461 agatacttta gaaccaccaa gtattgcaac gaacggacgc ttaggattat ttaccgcgcc
     2521 accaagataa tccagttctt tctgtagcaa gaatccgctg gcagattgtt tgacatggtg
     2581 agtgatgcct tctgtagaag cgtgcgcgcg atgggctgtt ccaaacgcat cgtttacgta
     2641 cacatccccg agagacgcta gctgctttgc aaagccagga tcatttcctt cttcttctgg
     2701 atgaaagcgc aggttttcta acagcagcac ttctccatct ttcaaggcag aggccgccgc
     2761 ttgagcttca tcgcccacgc aagttccaat gctttttaca ggcttaccaa gcaatttgcc
     2821 gaaagcgtcc gccaccgggc ccagacgcat cgagtctaca ggctttcctt ttggacgtcc
     2881 caggtgactc atgaggatta cccgggctcc gttggaaatg aggtactgaa cggtgggcac
     2941 acttgcgcga aggcgtttgt cgttttcaac tactccgttt ttgagaggca cattgaaatc
     3001 gactcgaacc agaacctttt tgccggcaac atttagatcc tttaccgtaa gcttgttcat
     3061 cgcatttgcc ctcgcgcttt tacttaaatc attttaccgt actgatgtgc tggatgaggt
     3121 ccacaagttt gtttgagtag ccccattcgt tgtcatacca ggaaacgagt ttcacgaaag
     3181 tatcgctcag ctgaattcct gcgcccgcat cgaaaatact ggtgcgtgga tccccgagaa
     3241 aatcctgaga aactacttcg tcttccgtgt aaccaagaat tcccttcaat tccccttcgg
     3301 aagcttcctt cattgctttc ttgatatcat cgtacttcgc aggcttggaa aggcgacaag
     3361 tcaggtccac tacagaaacg tctgctaccg gaacgcgaaa ggacatgcct gtcaattttc
     3421 cgtttagatc tggaatcact tttccaactg ctttggctgc gccagtggaa gaaggaatga
     3481 tattctgata cgcgccacga ccacctctcc agtctttgtg agaaggaccg tctacggttt
     3541 tctgcgtagc tgtgactgcg tggactgtgg tcatcaaacc ttctagaatt ccccaacgat
     3601 cattcacgac tttcgcaaga ggagccaggc agttggttgt gcagctcgcg ttggacacaa
     3661 tggtcatgtc tgctttgtaa gttttgtgat tcacgcccat gacaaacatg ggtgcgtccg
     3721 cagaaggggc agagataacc actctcttcg cgccggcttt gatgtgagcc tgtgcctttt
     3781 caatcgtggt aaagagaccg gtggattcaa ctacaacttc agcaccaata tctccccatt
     3841 tcaaattcgc aggatccttt tcagcagtga cgcgaacttt ctttccattt acaatgagtt
     3901 gtccgtcttt tgcttccaca gttcctttga aagcgcgatg agttgaatcg tatttgagca
     3961 tgtaggccat gtagtctgcg tcgagcaggt cattgattcc cacgatttcg atatcctgtc
     4021 tttccagagc tgtgcggaaa gccagtcggc caattcggcc aaatccgttg attccaatct
     4081 taagcatatg agtctcctgt attctaagtg gcttcgggct atacggtacg caagcggatt
     4141 ttcgccgtca tttcccgaca caaaacgtcg aaaaaatgcg tcccagaatt tcctcgtttg
     4201 aaatccgtcc tgtgatgcta ccaatatgat cgatcacgtc atcgatctca agggctgtga
     4261 tttcgtcagg cgcattctgc tgccagagtt ccatgatctt tgtcagcgtc gccttcatcg
     4321 ccagtagatg aaagcgatgt ctttcttcga gcaatatagg atcacgactt tccggtcttt
     4381 caagaactct atcccgaatc gcctcttcta catgatccat tcctgcacca gtaagacagg
     4441 aaacctgaat cgcgctccct ggatttgcgc ccggatgaag acgatctgct ttgttgatta
     4501 catggattac gttgtcgcgg tccgattcta caatcgaagc gtaggattcg cgtgaaccgt
     4561 ctattacgtg aatcacaaga gtgctttctt cgagctcacg ctggcttctg cgcactcctt
     4621 ctctttctac aatatcattg gtctcacgaa ttccagcggt gtccacgaga cgcacaggaa
     4681 ttccagcaat ctgcaactct tccgaaagag tatctcgagt ggtacctgcc acttctgtaa
     4741 caatggctcg atcgtagccg agaagtcgat tgagtagcga agactttcca gcattcggca
     4801 cacctacaat cgcaatagaa attccttctc gaatccttcc tgttgcactt ccgcgcgcaa
     4861 ggagcgaatc cagctctgcg atcagtccca taactcgtgc gcgcctttct tcgcgcgatt
     4921 gaaaagtgag atcttcggtg gaaaaatcaa cttccgcttc cgtctccgcc ttcaggccaa
     4981 tcatggccga cctgagaatg gacatcctgc gagaaagctc tcctccatag atagcgcgcg
     5041 cggccgaaag ctcatactca ctttttgctt caatcaacct cttcacagat tctgcgcgcg
     5101 tcaaatccag acgaccattg cgaaacgcac gcgaagtgaa ttctcctggc tcagcaagcc
     5161 gaatccccga ttcgactaac agtcgtaagg cgctcttcag taaaatggga tttccgtgaa
     5221 aggtgatctc ggctgagtct tctccggtgt aggaatgggg gcctttgaag taaacggcta
     5281 cgacctcgtc cacgagcaca tttgcgtcca tgaactttgc gaaaaatgaa tggcgcggct
     5341 gattagaaaa cggaataggc ttcaggtccg gtgcaacaac gaacctggaa ataatatcta
     5401 gagtacccgg accggacacg cgaatcgtac ccagggccgc gtggcccggg tacgttgcga
     5461 tcgccgcaat ggtcattggt cgtcgaaagc gtcctttcta ttaaaatcct gatcttcctc
     5521 ttcttcttcg cgaggagctc gattgaagtt tctttctctg tcctgacgat cagggcgatc
     5581 ctgacgatcc tgaggtccag aaccgggtct acctttctga ggaagaactc gcactctctt
     5641 gtatactccg tttccatctg atcgagtggt tactctctcg tctggttcga gcgcaagatg
     5701 aacgattcta cgttcatacg gattcatcca ttctagcaat acgctctggg aagtgcgcgc
     5761 cactcgatcg gccactgcct ttgcgagctt ggtcaatctt tgtttgcgac gctcgcggta
     5821 ttctgctacg tccagaacca cacgacgatt gttccggatg cgtgaatcca ccatgcaatt
     5881 ggtgagaaac tgcagcgaat cgagagttct tccctgcttt ccaatcaaga tcgcagaatc
     5941 ttcagaatca atggaaacca cgaagctttc ttcttcaata tggactccca cattctcaac
     6001 tgtcagaccc atcttcctaa tcagtgtgat cacggtgcct ttgatgactg tttcgatagg
     6061 aacgttcttc gcgaacaaac gaacaacggt tggtttcctg tttacaattc ccaggaaact
     6121 ctttccggcg gaaactgctt caaacgtaac atcttcttct ttcaatccaa gcgcgctcaa
     6181 ggcgatctgt tctgcttcag cgcgcgtctt gccttctgcc tcgtaaatgt aattcatact
     6241 cttactccaa aaatggttca ggcggtcttc ccgtcatcgg acatccggtt ggtcaccagt
     6301 tgccagacga tggcgagcac attctggaca gtccagtaaa gcgtcacgcc actgggcatt
     6361 gtccagaaaa agaaaatcat aatcacaggc atcatataca tcatgatctt ctgctgcgga
     6421 tccgtgatca cagttgtgag cctttgctgt aacaactgag aggcaaccat aaacagcggc
     6481 agaacattga tatgaaaatc ctttaggatc agaaagtctt tgatggtgta tattgtatca
     6541 ggcgcggata gatctgtcat ccacaggatg aacggactcc gccagagttc aatggactct
     6601 gaaaaggcgc tatagagagc aatgaaaacc ggaatctgga tcacaatcgg cacgcagcct
     6661 ttggcaggat ttacgttgtg cttcttgtaa acctccatgg ttctcttctg tttttccgcc
     6721 ggattgtccg catacttctc ctggattttt tccagctcag gctttagggc gctcattcgc
     6781 ttcatgctct tggcctgcat ctgattcagc ggaaacgtaa tcaacttgaa ggaaacagcg
     6841 atgacaatga tgacccatcc atagttgccc agatatacgt gaaggaatct catcacccat
     6901 acgattgcat cgcggacttt tgagaagagc gcaagaaaac tggaagtgta aatgactccg
     6961 cgtgcttctt tgtccgtgtc agacgatccg aattcagtcc tggccagcgc ttcgtttgca
     7021 aaagcatgac tttcgtctgt tcgcacgccg taatagattt cctgatccag aatgactgca
     7081 tcatttctct gctggatccc tttgatgtat tcatgaccgc ccgtcgtctg cgcccctgca
     7141 ggctgcaatt caagctcccc ggtgttgggc gcgagcctaa agtcgttgag aatcgtagtg
     7201 aatgcgcgct tgccgtccgg atcgatttcg tttctaatgg ctacgccatc cacctggctc
     7261 tgaggattgt ttgtatccag aaacttcgcg tatgcgaaga aatacttact gtgtgcgcca
     7321 acaaaatgaa gtgaattggg atgttccgca taaacactat aaggtttatc ttttagattt
     7381 tcaccgcaac caatgggagc aagaatgcta ttggtcggtg gagtattcat tctgctcttg
     7441 aattctccat tgtagtagta aaacctgccg tgcagagccg ggttgatccc tccggccatt
     7501 tctgtagagg gccctacatc cccaaagggg cgaaagaaca ttggaccgcc ccagacgaat
     7561 tcttttttgt ccaggtttcg tattacagcg atttgacgaa agaaatgctc tcctttcaaa
     7621 aagcgataga tcttaacaat ttcgagattt tgtccgcgaa gtcgaacagg aagacgaaaa
     7681 cgcacttcta cgacgccatc tttgtcctga aaaggtcctt ctttcttgaa gagcgctttg
     7741 ttcagggccg gatcaccgag ctgcgaggct ccgtctccat agtagtatcc atgaaattga
     7801 aagtcgaatc cgttcccccg gcttccctcg aaggcctggt aggattgcgc ttccggagtt
     7861 tccgttttca gtacggaatc aggaacattg agttctttgc ttttctttac gtagaactta
     7921 tcaattcgtc cgccctggct gttcaacttc acgatgaagt tgccagtatc tacagaaaca
     7981 atttccccgg gacccgactg aaattcaaaa tcggttaatt tcgcacccgt gccttcgtgc
     8041 tcctgagtca ccgggggatg agatccgctt ccgctgaatt gaaagaacag taggaccgca
     8101 ataccgatca gcagtactgg cagaaaagaa cccagtccgg gtttttgttc ttctttattt
     8161 tccattttgt ttacgcctgg ttttggggtt cgtctggaat aactggatcg aagtatccct
     8221 cattgaaggg attgcacctg agaatacgat agcctgattt gataaacgca tgcccgaatg
     8281 aaaaacgttc gaagcattct actgcgtaac tcgaacaact tggataaaag cgacaacgac
     8341 caggaatgag tttagaaata gtattctgat aaacgcgaat ccctgcgatg gccagagtgt
     8401 tcgcattcat ggggcaagca gccttgcacg aacaaacgca tcacgaagag tcgctgttct
     8461 ttccggaagg gaaagttttt cgaattcaag atcggcaaat actgcaacag aacacccggg
     8521 tttgattgcg ggaaccagag gctgaagtgc ggagcggata attctttttc ttctatttct
     8581 atccacagct cttttcatac gtgaagaaca cacagctatc ccgccaggat tgccggacga
     8641 tacgatcaac atgattcctc tggaatgcat ctttctgcct tcccggaaaa gcgcagaaaa
     8701 ctggctctct ccgcggaggg tacgggcgcg caggccggac agaaattatt tcctgtgtgt
     8761 ttgatcagaa acagtcaggc gcttgcgccc tttttttctt ctgtttgaga gaactttgcg
     8821 tccgttggcc gtagccatac gagaacgaaa gccatgagtt ttgtttttac gcttattgct
     8881 tggttgaaat gttcttttca tagggaaatc gtgatgatcc tgcgatcgat gttagtttaa
     8941 gtttcctgag tctggctgaa gatgagaaat atgatcaatc tcagactgtt tgatcaagaa
     9001 acctcgaaag aggcatcagg gtcaactgtt tttgaagaga ttacattttt ggcttgctga
     9061 cttctacgcc tcaaatttca ttccgccgtc catcatgatc aaagacgcgt ggaatcaggt
     9121 cctcaaagac ttcgcaggcg aatcctccca gaattcaagc ctgaaatggc tatattccgc
     9181 gaagcccgga accagtactg attcatcccg actgattctc aatgtgcccg ataatggaat
     9241 tctgtcacat ttgcgagaat acactcgccc gattgaagtg aagatggcca gcattttagg
     9301 acggccggtt cgcgttgaat tccaactatc ccattcactg ccggaatgga ttcatcccga
     9361 atacagcttc gaaacttttc tagaatcaaa atgcaattca aacgcgcttc aatgcattag
     9421 agccgcgtgt cgccaaccgg gcagacacaa tccaatcttt ttgtccgggc ccgccaattc
     9481 cggtaagact catctactac acagttatgc gagaagcgta atcgaagcaa agccaggcgc
     9541 acgcattgca tatctttcgg ggatcgcttt taagctgggg catcttcgcg ctatcgcttc
     9601 gaggtcttcc ctggatttca aagaatctat gatgtctttc gattgcgtca ttttcgatga
     9661 catccacttg atcgggagcg gcaagtccac tctagaagaa attgacttcc tgatcactgc
     9721 ttttctcgac cgaggcaaac aaatcgtatg ttccagttcc acagagccag gttctgtttt
     9781 cccttccggc agacttcgaa gcagaattct ttcgggcatt gttgttcaaa tggaa
//
LOCUS       DAOQJL010000081         9824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3642_length_9824_cov_4.510492, whole genome shotgun sequence.
ACCESSION   DAOQJL010000081 DAOQJL010000000
VERSION     DAOQJL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3642_length_9824_cov_4.510492"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="PLG78_05385"
     CDS             complement(<1..1649)
                     /locus_tag="PLG78_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE22575.1"
                     /translation="MNTPSKKSNRLAKEKSPYLLQHAHNPVDWYSFGEEAFAKAKSED
                     KPILLSIGYATCHWCHVMERESFEDEETANYLNEHFVAIKVDREERPDVDAIYMRALH
                     ATGQHGGWPLNMFLTTDLRPITGGTYFPPKPAYGRPSFLQVLQTIADLWKKDRAKMLE
                     SADSLTGFLQTRENQTGNIPDSGVFAQAASHFRNSYDELRGGFLSNGPNKFPPSLNML
                     FLLRHFEHTGDESILAMVENTCDAMKRGGIYDQIGGGLSRYATDHDWLVPHFEKMLYD
                     NALFARVLTETYRITGNERYKTWALDIFEYVARDLTSPDGAFYSAEDADSEGKEGLFY
                     LWSADEIDSLLKEHSFSAETRKQIARFYGVSMRGNFEGKNILHEASVREEFLRAHNLK
                     AAIWDETIGKVRHLLLLERSKRIRPLRDDKVLTAWNALMVSALARAGAVFGDAGLTAR
                     AEKAAAFIESHMVQGETILRRFREGEARFEGSLQDYAGLALSFIDLYQTTFRAHYITR
                     AMQIAQVIATKFKAEGGGFFDTADGIPDLLVRSMEIYDGVEP"
     gene            1709..2254
                     /locus_tag="PLG78_05390"
     CDS             1709..2254
                     /locus_tag="PLG78_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22576.1"
                     /translation="MINQYLARVLFLSVVLLAAQCSSSQTQENKPQERGQVPTTGCIQ
                     GDCTAGDGTFVYNGGDKYLGSFKNGLREGKGTLDYASGDQYIGQFVADKRSGDGTYKF
                     ANGDVYVGQFKDGERAGNGVYTFKEGPVFEGTFANDGKSGNGSLKAGDKLKRCELQDR
                     KIVCEADKAEAPATETKTDKK"
     gene            complement(2478..4760)
                     /locus_tag="PLG78_05395"
     CDS             complement(2478..4760)
                     /locus_tag="PLG78_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE22577.1"
                     /translation="MPSTQDILIHAFHILLFLAASAIFVGAVVYRLKTVAKGQKAATL
                     ADMGARIRSFIFNVVLQQKLFKHPVRGAMHAFIFYGFLVYGLHTMSQMVAGNLWSFFK
                     SSGIEPYEFLIPDYIPAILTFKDPNQVLLCIAGLLITMTLTFLLYNNLKIGKKFKANR
                     NAGSEWFFAILLLAQFGLAFGLITSAGTEVFEMLIQNMSALVLTGLVFFAYRRWALRS
                     KGLDVPSTQSAIVLLMIGTLMVSTLTGGAAQSVISGKGTHTWVSSSVLKLLTALGMSG
                     GAEALAVRNFAWWAHIVTVYGFMVVVPLSKHSHLIFAPMNFFMIRGTPRGQMTHMDME
                     NAAVYGAGNVRELPWTSMLDGLSCIECGRCTVQCPANRTGKPLDPKKIMVDIKHSMIE
                     NTNTLLKTEDPAAALIGDPFITEEELWGCTSCYACVEACPVGNNQLEAILEMRRNLVL
                     AESRFPSHLQLAFQNMENNSNPWGVGAHTRADWCADLNVKTMAEDSNVDVLYWVGCAG
                     SFDDRNKTIARSFVKIMQGAGVKFSILGTEENCTGDSARRGGNEYLFQTLAQTNIETM
                     NRYNVKKVVTACPHCYNTLKNEYPQMGGNYEVQHHTDFIDDLIRDGKVEMDPATVEDM
                     KSRRGTYHDSCYLGRYNDVYDQPRNIVEQTLGRGIPEASDHHSTSLCCGAGGAQMWME
                     EQYDRVNVKRTNQLLETGADTIATACPFCMVMITDGVKAAGMQDQVQVLDVAELVAAS
                     MKGGTGAIPHAHGKAEPAHH"
     gene            4843..5919
                     /locus_tag="PLG78_05400"
     CDS             4843..5919
                     /locus_tag="PLG78_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNE22578.1"
                     /translation="MIHSPESILARAEQGERITPEEALTLYREGDFLAIQKTARELRN
                     RRHDPGVATYTMFRVVNYTNFCNVECTFCSFMDEIGSGKGYVLTTDQIRDKMKESLEI
                     GCDQMFLQGGVYPELPFEYYTDVLSATKSLGDIHIRGFSPVELISMEKTFGMSLREVV
                     RKLKAAGLDSVPGAGAEILTERMRQVLSPKKTTVSEWVRALETCHEEGLLGSANVVFG
                     SEETQEEVIEHLRVVRELQDRTHGFLSFIPWTFQQQTKRFKVRNIPATEYLKVLGICR
                     IFLDNIDHIETSLMVLGTGVGCIALHGGADDISSVILEENVLRSYGLKSEEKVLDFLR
                     DSGFRPQYRTLMYEHKDRKIPQAA"
     gene            6039..7334
                     /locus_tag="PLG78_05405"
     CDS             6039..7334
                     /locus_tag="PLG78_05405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE22579.1"
                     /translation="MAFVPEYILILAATIAVDFYAGIMIGRAEGKKKANYLMLSVLVN
                     IAFLFVFKYYGFFRENVNSAVGSDLIPLYKIVLPIGLSFHTFQSLSYIFEVYYGRQVP
                     VTHFGRYALFVMYYPQLVCGPIERPFNLLKQFLVERRFLFSRFQSGMTLMLWGFFKKL
                     VVADRLGMFVDRVWKEPGSFGGIYLFLAACFFSIQIYCDFSGYTDIARGASRVMGIRL
                     MLNFDLPFFSKSIPELWRRWHISLSSWFRDYVYIPMGGGKVSTVRKHFNVMVLFLLSG
                     FWHGANWTFIIWGGFHGVLTVLSQVTAGFRQWFLRATMLDRIPRVMDIWRIVLTFLLF
                     SLGAIFFRAKTLSDSLTFIAGIPSLSLKLPADIRIPYDIKSGLLVLGILVFIETLLVA
                     GSLNRYFRKYRLIPLQGLLLAAILVAGVFEKQSFIYFQF"
     gene            7371..8342
                     /locus_tag="PLG78_05410"
     CDS             7371..8342
                     /locus_tag="PLG78_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22580.1"
                     /translation="MKAPYGKIAKKTGLWLGLLLISALAFDRILYRALFPLYDRRSTL
                     SGMNDELDGFIIGSSQTRWAVDGGVISQRTKSRFAVFAPPGANLELRSAMLRDYVERF
                     ADHPPSYIVLEGHPFSFHPTRYPDDAYKTLLGYRPRGILREYLSQRYGDDPLFQGTKV
                     FHSLALNSEFYFLASAVVDLPIRAIRPVSELPQEPDLPMETRIAQWRAYYAELKDRGA
                     VDPRFVSAYADLMQVISRTGIPIIVLDLPIYRLDPQMEKDLSVGRRILQKDLPPNVHY
                     VRFEADRVENDPTYFYDASHLNAKGRAWLSEKLADEITRTLAQSKSK"
     gene            complement(8386..>9824)
                     /locus_tag="PLG78_05415"
     CDS             complement(8386..>9824)
                     /locus_tag="PLG78_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HNE22581.1"
                     /translation="IDQVTVKRVYDPASPGFGADIITIDPKANFAHINAIQRIIAGYT
                     QAAFEYTPADSETVSKFILYYNAKQRGALDKFKGKYSEPVIQSLSPETVGIDRSYRNW
                     SGKTQIVIPLVKNVVRPGKQDVNTTEIKKETDKTTKAEDKKQLTDIENRRKDETTKKL
                     DQKQDQLKQEQKLLEDKKQDIQKQQQQVQQQKQDTGKKLQELAKDPIKNATEIKKEEQ
                     KQQDLKKQEDNLKKEEQKVTQQVNENKTSQAEVTQQKQDQNKTDTATNTATKTEDQKK
                     VDTLQKENDELKKQQDAKEQKSDNVVGEKILFLRFVKFHSEGHYSNELWMLDPVKDDT
                     LYKSPYTNICGREFVVVPGQGVLVIGYEGTAADSKEHLLALLDQDKLTLKTKSKENIY
                     WRSPLILRDNKIYGIEVKDGQYYLTRFNPDLSFDARTSEPINADSDITFYNDKIYVTG
                     KAQGTGATTIKAYQRSDLKHLKTITPPN"
BASE COUNT         2195 a         2341 c         2514 g         2774 t
ORIGIN      
        1 ggttccactc catcatagat ttccatagag cgaaccagta gatctggaat tccatccgca
       61 gtgtcaaaga agccaccgcc ctccgctttg aatttagtag caatgacctg tgcgatttgc
      121 atcgctcgcg tgatgtaatg ggcgcgaaag gtggtttgat aaagatcgat gaaggagagg
      181 gcaagtcctg cgtaatcttg aagagatcct tcgaatctgg cttctccttc tctgaatcgc
      241 ctcaggatgg tttctccttg caccatgtgc gattcaatga acgcggctgc tttctctgcg
      301 cgcgcagtga gtcctgcgtc tccaaacact gcaccggcgc gcgcgagtgc agataccatc
      361 agagcgttcc aggcagtcag taccttatcg tcgcgcagag gtcgaattcg tttggaacgt
      421 tccaatagca aaaggtgtct gacttttccg atcgtctcgt cccatatcgc ggcttttaga
      481 ttgtgcgctc taagaaattc ttcgcgaacg gacgcttcgt gcaaaatgtt tttgccttcg
      541 aaattcccgc gcatgctcac gccgtagaac cgtgcgattt gcttgcgagt ctctgctgag
      601 aaagagtgct ctttgaggag tgagtcgatc tcatctgcgc tccacagata gaaaagccct
      661 tcttttccct cactgtctgc gtcttctgca gagtaaaacg cgccatccgg agaagtaaga
      721 tcgcgcgcca catattcgaa tatatccaga gcccaggtct tgtagcgctc attcccggtg
      781 attctatagg tctccgtgag aacgcgtgca aaaagagcat tgtcataaag catcttttca
      841 aagtgtggaa ccagccagtc gtgatcggtt gcatatcgtg agaggccgcc gccaatttga
      901 tcgtaaatgc caccacgctt catcgcgtcg catgtattct ccaccatggc aagaatcgat
      961 tcgtctccag tgtgttcgaa gtggcgcagt agaaagagca tgttgaggct gggcggaaat
     1021 ttgtttggtc cgttggatag aaagccgccg cgcaattcat cgtagctgtt acgaaagtga
     1081 gaagctgcct gtgcgaaaac gcctgaatcc ggaatgttac cggtctgatt ttcgcgcgtt
     1141 tgaaggaatc cggtcaagga atctgcggat tcgagcattt ttgctctgtc ttttttccag
     1201 agatctgcga tggtttgaag tacctgtaag aaacttggtc tgccgtatgc tggcttaggt
     1261 ggaaagtacg taccaccggt aatcgggcga agatctgttg ttagaaacat attgagcggc
     1321 cagccaccgt gctgtcctgt tgcgtggagt gcgcgcatgt aaatggcgtc cacatccggt
     1381 ctctcttcgc gatctacttt gatggcaacg aaatgttcgt tgagatagtt cgcagtttct
     1441 tcgtcttcga atgattctct ttccattacg tggcaccagt gacatgtcgc gtagccaatg
     1501 gagagcaaga ttggtttgtc ttccgattta gctttcgcga aagcctcctc tccgaagctg
     1561 taccaatcca ccggattgtg cgcatgctgg agcaggtagg gacttttttc tttcgcgagg
     1621 cgatttgact tttttgaagg cgtattcatg cgcttttttt tccttgcatt cgaacagaac
     1681 cgggtcatct ccgacccagg agacttctat gatcaatcaa tatttagcca gggtcctttt
     1741 tctatccgtt gttcttctgg cagctcagtg ctcttcttct caaacacaag aaaacaagcc
     1801 tcaagagcgt ggccaggttc caacgactgg ttgcatccag ggtgattgca cagccgggga
     1861 tggaacattc gtctacaacg gcggagataa ataccttggt tctttcaaga acggacttcg
     1921 cgaaggcaaa ggcactcttg attatgcatc cggtgaccag tatattggcc agtttgtcgc
     1981 agacaagaga agcggtgacg gaacatacaa atttgcaaac ggtgatgtct acgttggtca
     2041 attcaaagat ggcgagcgtg caggaaacgg agtctatact ttcaaggaag gtccagtttt
     2101 cgaaggaaca tttgcaaatg acggcaagtc aggcaacggt tctctgaaag caggagacaa
     2161 actgaagcgc tgtgaattgc aagatcgtaa gatcgtatgc gaagcggaca aagcagaagc
     2221 tccagccact gaaacaaaaa cagacaagaa gtaatccttc cagaaagccc gcccaacctg
     2281 ggcgggccgc attccgaatc ctcacacgaa caaatcaaat acagccctcg ggcaaccctg
     2341 gcagccagtg cgcgctcacg cttctggaag cttccagtgg aatttacaga gacctgcaga
     2401 gacagagaac ctgcgaggga atcgatccgg gggagagtgc gcgggcggca aatgccgccc
     2461 ggtcgtaatt gaatggttta gtggtgagct ggttccgctt tgccgtgagc gtgagggatg
     2521 gcacctgtgc ctcccttcat gctggccgcc accagttctg ccacgtcgag cacctgcacc
     2581 tgatcctgca ttccggctgc tttcacaccg tctgtgatca tgaccatgca gaatgggcag
     2641 gcagtggcga tcgtgtctgc tcctgtttcg aggagctgat tggtacgctt cacgttgact
     2701 ctgtcgtatt gttcttccat ccacatctgg gcgccacctg caccgcagca aagagatgta
     2761 gagtgatggt cggacgcttc tggaatgcca cggccgagag tttgttctac aatgttgcga
     2821 ggttgatcgt atacatcgtt gtaacgtccc aggtagcaag agtcatggta agtaccgcga
     2881 cgagacttca tatcctctac agtcgcagga tccatttcta ctttgccatc gcgaatgaga
     2941 tcgtctatga aatcagtgtg atgctgcact tcatagtttc cacccatttg cgggtattcg
     3001 ttcttcagtg tgttgtagca atgtggacac gcggttacaa cttttttcac gttgtaacga
     3061 ttcatggttt cgatgtttgt ctgtgcgaga gtctggaaga gatactcgtt tccaccgcgg
     3121 cgtgcgctat ctcctgtgca attttcttcc gtgcccagga tgctgaactt cacgcccgca
     3181 ccttgcatga tcttcacgaa agaacgtgcg atggttttgt tgcgatcgtc aaaagatcca
     3241 gcgcatccaa cccagtagag tacatctacg tttgaatctt ctgccatggt cttcacgttc
     3301 agatcagcac accagtccgc tcttgtatga gctcccacac cccatgggtt ggagttgttt
     3361 tccatattct ggaacgcgag ctgaaggtga gaaggaaaac ggctttctgc tagaaccagg
     3421 ttgcggcgca tttctagaat ggcttctagc tggttgtttc ctacagggca ggcttctacg
     3481 catgcgtagc aggaagtaca tccccacagt tcttcttctg tgatgaaagg atctccaatg
     3541 agtgctgctg ctggatcttc tgttttgaga agagtgttcg tgttttcgat catggaatgc
     3601 ttgatatcca ccatgatctt ctttggatcg agaggtttgc ctgtgcgatt ggcaggacac
     3661 tgcactgtgc atctgccgca ttcaatgcag gaaagaccgt cgagcatgct ggtccaggga
     3721 agttcgcgaa cattgcctgc tccatagact gctgcgtttt ccatgtccat gtgagtcatc
     3781 tggccgcgcg gggttccgcg aatcatgaag aagttcatgg gggcgaagat gagatgagaa
     3841 tgcttggaaa gtggaaccac taccatgaaa ccgtatacgg ttacaatgtg agcccaccat
     3901 gcgaaattgc gcactgcgag agcttccgct ccaccgctca tgccaagtgc agtgaggagc
     3961 ttgagaacgg agcttgaaac ccaggtatgt gttcctttgc cgctgattac ggattgcgcc
     4021 gcgcctccgg tgagagtgga caccatcagt gttccaatca tcagaagaac aatggccgat
     4081 tgcgtagatg gaacatcgag acctttggaa cgaagagccc atcttctata agcaaagaat
     4141 acgagcccgg ttagaaccag agcagacata ttctgaatga gcatttcgaa tacttccgtt
     4201 ccagcggatg tgatcagtcc aaacgcaaga ccgaattgtg cgaggagtag aatggcgaag
     4261 aaccattctg atccagcgtt gcgatttgct ttgaacttct ttccaatctt gagattgtta
     4321 tagagaagaa aggtcaaagt catcgtgatc agaagaccgg cgatgcacag aagcacctgg
     4381 tttggatctt tgaaagtcag tatggctgga atgtaatccg gaatcagaaa ttcgtagggt
     4441 tcaatgccgc ttgatttgaa gaaactccag aggttcccgg ccaccatctg gctcatggtg
     4501 tggaggccgt ataccaggaa gccgtagaag atgaatgcgt gcatggctcc gcgcactgga
     4561 tgcttgaata gtttttgctg cagaaccaca ttgaatatga acgatcggat gcgagcgccc
     4621 atatccgcga gagtcgcagc cttctggcct ttagccaccg tcttgagacg gtaaaccact
     4681 gcgcccacaa agatggcgct tgcggccagg aaaaggagaa tgtgaaaagc gtgaatgagg
     4741 atatcttgag tcgaaggcat agctaaaacc agggatatcc attcaggaat ttcgtcgaga
     4801 aaaaagctgt tcctctagcg cgctctgcgg cttctgaacg ttatgatcca ttcgccggaa
     4861 agtatactag cccgggcgga gcagggcgaa cgcatcacgc ccgaagaggc tttgaccctg
     4921 tatcgcgaag gggacttcct tgcgatccag aagacggcca gagagctgcg aaaccgtcgt
     4981 catgatccag gagtggctac ctacaccatg tttcgcgtgg tcaactacac caacttctgc
     5041 aacgtagaat gcactttctg cagttttatg gatgaaatcg gatccggtaa gggctacgta
     5101 cttaccacag atcaaatccg cgataaaatg aaagagtccc tggagattgg ctgcgaccag
     5161 atgttcctcc agggtggtgt ctatccagag cttccttttg agtattacac ggacgttcta
     5221 tccgctacaa aatctctcgg ggacattcat atccgcggct tctctccagt tgaattgatc
     5281 agcatggaaa agacgttcgg catgagtctg cgcgaagtgg tgcgaaagct caaggctgcc
     5341 ggtcttgatt ctgtgccagg cgccggcgct gagatactta cagaacgcat gcggcaggta
     5401 ctttctccca agaaaacaac cgtgtccgag tgggtgagag ctcttgaaac ctgccacgaa
     5461 gaagggctcc tgggcagcgc gaacgttgta tttggttcgg aggaaaccca ggaagaggtg
     5521 atcgaacatc tgcgcgtcgt aagagagcta caggatcgga cgcatggctt cctctctttc
     5581 attccctgga cgtttcagca gcagacaaag cgattcaagg tgcgcaacat tcctgcaaca
     5641 gagtatctca aagtgctggg catctgtcgt attttcctgg acaacattga tcatatagag
     5701 acgtctctta tggtgctcgg aacaggagtt ggttgcattg cgctgcacgg aggcgcggac
     5761 gatatttctt ccgtgatcct tgaagagaac gtgctgcgaa gctatggact caaatcagaa
     5821 gagaaggtac tcgattttct gcgcgacagc gggtttcgtc cgcagtatcg cactctgatg
     5881 tatgagcaca aggatagaaa aattcctcag gccgcctagt cgtgctgttt aactcactcg
     5941 catttctcat attctttccg gcagtaacga tttcctattt tctgctgccg catcgattca
     6001 ggtggctgct tttgcttctg gcgagttgct acttttacat ggccttcgtc cctgagtaca
     6061 ttttgattct ggccgccacc attgccgtgg atttctacgc ggggatcatg attggccgag
     6121 cagaagggaa gaagaaagca aattacctga tgctcagtgt gcttgtaaac atcgcattct
     6181 tatttgtttt caaatactac ggattctttc gcgagaacgt aaatagcgcg gtcggctcgg
     6241 atctgattcc cctctacaag attgttcttc cgattggtct atctttccac acgtttcagt
     6301 ctctcagtta catttttgaa gtatactatg gccgtcaggt tccggtcact cattttggac
     6361 gttatgcgct ctttgtaatg tactacccgc agctcgtgtg cggacccatt gaaagaccat
     6421 tcaatctact caaacagttc cttgtggaga gacgcttcct gttctctcgc tttcaatccg
     6481 gaatgactct catgctctgg ggttttttca agaaactcgt tgtggcagat cgtctgggta
     6541 tgtttgtaga tagggtctgg aaagagcccg gttcctttgg tggcatttat cttttcctcg
     6601 cagcttgctt cttctcgatc cagatttatt gcgatttttc cggctatacg gacattgcgc
     6661 gtggagcaag tcgcgtgatg ggaattcggc tgatgctgaa ttttgattta cccttctttt
     6721 cgaaatcgat tccagagctc tggcgcaggt ggcacatttc tctttctagc tggtttcggg
     6781 attacgtata cattcctatg ggcggcggca aggtcagcac cgtacgcaag catttcaatg
     6841 tgatggtgct cttcttgctc agtggtttct ggcatggggc gaactggacg tttattatct
     6901 ggggtggatt ccatggtgtt ctcacagtgc tttctcaggt gaccgcaggg tttcgccagt
     6961 ggtttcttcg tgcgaccatg ctggatcgaa ttcctcgggt tatggatatc tggcgcatcg
     7021 tattgacatt ccttctgttt tccctgggag ccatcttctt tcgtgcgaag accctctcgg
     7081 atagtcttac tttcatcgcc gggattcctt cactgtcctt gaaacttcca gcggatatca
     7141 ggattccgta cgacattaag tccggattgc ttgtgctagg catcctggtt ttcattgaga
     7201 cgcttctggt tgcgggaagt ttgaatcgct actttagaaa atacagactc attccgctcc
     7261 agggcttgct tctggctgcc attctcgttg ccggtgtttt cgaaaagcag agtttcatat
     7321 actttcagtt ttagatagtt gatgtttata gaatatatga tggatgcagt atgaaagcgc
     7381 cgtatggcaa aattgcaaaa aagacgggtc tatggcttgg tcttctactc atcagcgctc
     7441 ttgcgtttga tcgaattctg tatcgtgcgc ttttcccgct ttatgatcga agaagtactc
     7501 tgtccgggat gaacgatgag ctggacggat tcatcatcgg aagcagccag acaagatggg
     7561 ccgtggacgg tggcgtgatt tcacagagaa caaagtcgcg gtttgctgta tttgcgccac
     7621 cgggtgcgaa tctggaactg cgttccgcga tgctgcgaga ctacgtagaa cggtttgctg
     7681 atcatccgcc atcttacatt gttttggaag ggcatccttt ttcatttcat cctacaagat
     7741 atccggacga cgcttacaaa actcttcttg gttacagacc gcgcgggatt ctccgcgaat
     7801 atctgtcgca gcgctatggg gatgatccgc tatttcaggg aacaaaagta tttcattctc
     7861 tcgcgctgaa ttctgagttc tatttccttg cgtctgctgt ggtggatctt ccgatccggg
     7921 ccattcgacc ggtaagtgaa ttgccacagg aaccggattt gcccatggaa actagaattg
     7981 cccagtggag agcttattac gcggaactca aagatcgtgg ggctgtggat cctcgctttg
     8041 tgtctgctta cgcggatttg atgcaagtca tatcccgcac tggcattccc attatcgttc
     8101 tggatttacc catttatcgc ctggatcctc agatggagaa agacctgtca gtgggaaggc
     8161 gaatcttgca aaaggatctt ccgcctaacg tccattatgt gcgattcgaa gcggacaggg
     8221 tggagaacga tccgacgtat ttctatgatg cgtctcattt gaatgcaaaa ggcagagcat
     8281 ggctcagtga aaagcttgca gatgaaatca cacggacgct cgcgcagtcc aaaagcaagt
     8341 aggtgtaaaa tacaccgggc tccgtgaccg gagcccggta acctctcaat taggaggagt
     8401 aatggttttc aggtgcttca gatcagagcg ctggtaagct ttgatcgtcg tagcccctgt
     8461 gccttgcgct ttgcctgtaa cgtagatctt atcattgtag aaagtgatat cggagtctgc
     8521 attgattggc tcgcttgtgc gtgcatcaaa ggacagatcc ggattgaatc tggtcagata
     8581 atactggcca tctttcactt cgattccgta gatcttattg tctctcagga tcaatggaga
     8641 acgccagtag atgttttctt tggattttgt tttgagtgtg agtttgtcct ggtccagaag
     8701 tgccagcaaa tgctcttttg aatccgctgc agttccttcg tacccaatca caagcactcc
     8761 ctggcctgga accacgacga attcgcgtcc gcagatgttc gtatacgggc tcttgtaaag
     8821 ggtgtcgtct ttgactggat cgagcatcca cagttcattg gagtagtggc cttcgctgtg
     8881 gaatttcaca aagcgcagga acagaatctt ctctccaacc acgttgtcgc ttttctgttc
     8941 ttttgcatcc tgttgtttct tcagttcatc gttctctttt tgaagagtgt ctactttctt
     9001 ctgatcttcc gttttcgtcg cagtgttagt tgcagtatct gttttattct gatcctgctt
     9061 ttgttgcgtc acttcagcct gagaagtttt gttttcattt acttgctgag taactttttg
     9121 ctcttctttc tttagattgt cttcctgctt cttcagatcc tgctgctttt gttcttcttt
     9181 cttgatctca gttgcattct tgatcggatc tttcgcaagc tcctggagtt tctttccggt
     9241 atcctgtttc tgctgctgaa cttgctgctg ttgtttctgg atgtcctgct tcttgtcttc
     9301 tagaagcttt tgttcctgct tgagctggtc ctgcttttga tcgagcttct tggttgtttc
     9361 gtccttgcgg cgattttcaa tgtctgtgag ttgctttttg tcttctgctt ttgtggtctt
     9421 gtctgtctct ttcttgattt cagttgtgtt tacgtcttgc ttgcccggac gaactacgtt
     9481 cttcaccaga ggaatcacga tttgagtttt tcctgaccag ttgcgataag accgatcaat
     9541 tccaactgtc tcaggagaga gagactggat gactggttcc gaatactttc ctttgaattt
     9601 gtcgagcgct ccgcgctgct ttgcattgta atagaggatg aacttgctga ctgtttctga
     9661 atccgcagga gtgtattcga aggcagcttg cgtgtaacct gcgataatcc gctggattgc
     9721 attgatgtgt gcgaagttgg cctttggatc aatcgtgatg atgtccgctc caaaccctgg
     9781 actggccgga tcgtagactc tctttactgt gacctgatcg atcg
//
LOCUS       DAOQJL010000082         9811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3651_length_9811_cov_4.730525, whole genome shotgun sequence.
ACCESSION   DAOQJL010000082 DAOQJL010000000
VERSION     DAOQJL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_9811_cov_4.730525"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="PLG78_05420"
     CDS             <1..2405
                     /locus_tag="PLG78_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNE22582.1"
                     /translation="EGIPFIPRKEIQSTREDGESFTQHEMDLDSILRRKPAIVLVDEL
                     AHTNAPGSYHPKRYMDVEDLLEGGIHVYTTLNVQHLESVADFVEQITGAAVQERVPDR
                     ILDLANEIELIDISPDELRKRLHEGKVYRSDRAEVAARNFFKPENLLALREMALQTTA
                     RVVDTESLKRRTSGEVLRTRERILVLVGTDRFAARLIRIGRRMAADLKAPWFVMHVRS
                     SHSRGEDNSRIESEFNLARSLGAEVLLMDGDLVDAVEQVIAERSITQMLVGRTEHRFS
                     FSRPITHRLIDRVRNADIHIVPIKREINARKRRSYRLPELRSPFKEYAVASGVIWTLT
                     VGCLVAFPDGQYSTPSIIFIAAIAALGLVFGRGPVLSAAVQSAFAWDFLFIPPRYTLT
                     IGKLEDRLMFGLFFAVAIVTGILTSRLRRSKADLLRRQRDLVQIQEISQALAGATGVE
                     EITGLVCNKLSAIFDIPDCMILLKQGKISTMLPATASIDAKEQAVADWVVENGKPAGK
                     GTDTLPLSAALWIPLKTPSRGTMGALRLPVENPDINELNLVGAIAAQASLALERELLA
                     SSARQAAVNEESEKLFHAILRSISHELRSPVTAMLAAAENLSRDRVMMDDLLRNEVRQ
                     EISDSSQRLARVVDQLLAMSRIESGRLTLQLENHDLRDLLQECMAQLGKEFAGHTVKI
                     DASEKVEARFDFPMMRQALIGVLHNAALHTPIHTIVQIWIFRERASAVIIIEDNGPGI
                     PGDPGKLFEPFFRGAAAKPGGLGLGLSIAKSVVELHGGSIVATRTDTGSRFRIELPLE
                     KS"
     gene            2402..3076
                     /locus_tag="PLG78_05425"
     CDS             2402..3076
                     /locus_tag="PLG78_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE22583.1"
                     /translation="MKEKILLIEDEKPIRKMLSINLESEGYTVLEADSGKEGVQVCAR
                     NLPDLVLLDLMLPDMQGQEVLRMLREFTVVPVIVLSAVSSEKSKIELLDRGADDYLTK
                     PFSPGELLARVRVGLRHAQSSKTESVLEAGPIRLDPSDRTVQIRGNQIHLTPTEFAIL
                     ALLLQYAGKTVTREILIQTIWGSNENETGSLRVHINQLRKKIETSAAMPELILNEPGV
                     GYRLAV"
     gene            complement(3097..3672)
                     /locus_tag="PLG78_05430"
     CDS             complement(3097..3672)
                     /locus_tag="PLG78_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22584.1"
                     /translation="MHSEFYQIGIVVAILIAGYFYWRNEKLKEKIKDLSGTSGGVPST
                     VSQFRSQQNAEEPQLSIPEDESAKPEANPEEEKKEYNYRDPKFGMPPIASTKRILGRE
                     ENKFGDAVYGRDSGYDSNAYIGRVSRGAELELLDRRIYKNEFEIDIIKVKVLSNEWAT
                     EVGQTVWLGLNDTSFRSMVSQDGSEIQEPRE"
     gene            complement(3707..4195)
                     /locus_tag="PLG78_05435"
     CDS             complement(3707..4195)
                     /locus_tag="PLG78_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNE22585.1"
                     /translation="MSEPIQLIREEMFDSLAAEARAKPRLRINHNFHDSMDDNPHRFL
                     NVLLKGTYLCPHRHQFPPKAESFLILQGSIAFFIFDDSGVVQQIHTLSADGPVRGIDI
                     APGIWHGLLVLSERCVCFEVKPGPYRPSDDKEFASWAPREGDPRVGEMLARLEREYAL
                     RK"
     gene            4194..5234
                     /locus_tag="PLG78_05440"
     CDS             4194..5234
                     /locus_tag="PLG78_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNE22586.1"
                     /translation="MSEVLKSEENPYRTQSASLESDGRTVYLYLNPVEGVDLVPRAVW
                     VMNLVPAPAEDADGMIRGQAPVRSRAACAHPNGMKAPHENEIDFVWFQEGTGVTLCVE
                     GEIWAVIPPFSGSDGFYGFCREATLPEHGTFPLPDGMKARAKENLDFWNRRTQKGFWA
                     QFQGNLLRTYESAFGPHNNYYVLDNGKFPAMGLAEFIIPDTTLRLFATIGMSAQNMPG
                     VELHVKNPEPLFRVELLTVRDAPHPWFIDSMSRVALLPWRTGGWVGPGHSIQFGRSEP
                     MSDYLVLEHHLVQDKILADLFAKLPSVFLDERYARTFLFLWPAVPDDFRVNQARGREF
                     LAKKNSVVERFS"
     gene            5231..6274
                     /gene="rsgA"
                     /locus_tag="PLG78_05445"
     CDS             5231..6274
                     /gene="rsgA"
                     /locus_tag="PLG78_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNE22587.1"
                     /translation="MISLDPRFEEDFKKIRDSGFEPGRVVSQTVTYEVATESGDYTAQ
                     PAGKLLFSGMLPAVGDWVALQVYEGGAIIHDIVPRKTVIMRKSVGKSSADQVIAANLD
                     YAVVVMGLDSNFNLRRLERYTTIIAASGAKGIIVLSKADLADPEEIKLKVDHTIACAP
                     GLPVIVWSDADKRGLEELMAILRGPATFCLLGSSGAGKSTLINAIAGKPIARTGAVRT
                     SDEKGKHTTTVRTLISLGNGIDLIDNPGMREIGLTSEEGLSDAFPEITQAAAECKFQD
                     CTHENEPGCAVELAVQEGRITAERFASYMRLLHESRTIESMKSERGRQKKKQNEKTLA
                     KMIRARLKDKGRS"
     gene            complement(6252..8105)
                     /gene="recD"
                     /locus_tag="PLG78_05450"
     CDS             complement(6252..8105)
                     /gene="recD"
                     /locus_tag="PLG78_05450"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNE22588.1"
                     /translation="MSRTVREAARENPELYLWVSLVDDLLEFSHFPGDESIRALLLLL
                     ADTCMHGDLCMRLDDLSLTSTRDRFVQRHDTTEPNLEDAIVAAKSINRYSPLIGQDEK
                     SPVVLSDTFLFFQKYFLLRRNIERDFARMAAQPPRPLEKAKEAYGTALSVSGLTLSAE
                     QKLAVALSLFSSFVIITGGPGTGKTTVIRAVLDSFLRAGIPADRVILCAPTGRAARRM
                     AETLGDQYTASTIHTALSYRRDSQDFYFGPNRKLIGDVLVVDEVSMVDVDLMSRLLAA
                     VDPTRTRVILIGDRNQLPSVDAGAVLGDLVELFALSGNPQYSPDFASWLRTLDPELDL
                     PQMPGKSRAVDRMVHLTASHRSADVVIRASDAVRKAEESLPWSIVPLRELPENHGVVQ
                     IAFRDGGQNEVLGAWLKLSYGPEFVDLLREASALDADDPAAVDVMRHLTEVTGRARIL
                     SALRQSEDGSVRLNRRMMKLFLKQNRSVMFGEWFSGLPLMITRNDYSRELFNGETGLL
                     VRFRTGFRWIAARGEEIRLLPPESLPECEPAFVTTVHKSQGSEYNRVLLWLPESDHPM
                     VSRQILYTALTRAKELVWIYGKESVLRAGIQRNPRRETGGLSLDSMNDPYP"
     gene            complement(8102..>9811)
                     /locus_tag="PLG78_05455"
     CDS             complement(8102..>9811)
                     /locus_tag="PLG78_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE22589.1"
                     /translation="LMLVACFTLRMDDIKEAMLTNFFRVQPIDLVNSDVPERVLSQFR
                     QWNTLALEGKIARLLRSMMVDTLALLPGNAENVLAGEQLDPVDDYPRRRANLEHIARD
                     IEILCRGTTLSEALATLRARINDQTTADEESDLHHEESDLDRVRIMTIHASKGLDFDC
                     VFIAGGFGEFNGQAYLDYFDPGGSGARIFDLAASTRDVFKIQRFEEYKRLYYVAFTRA
                     RDRVFAPFFPSGRGGPLSHLVAPAADQVLAQNLARLVQPMHAGPPEPLTRTAKAPGRT
                     ELSRFPTNLSVRAAGLTSYSGILRRAVALDALRAEFGDTNPAEDEPLPLPETRDPTLD
                     QKGNFLIVDHPELPRGEATGNAIHEALEGLDFSEFDRKQVPKPGTLEPATRDHIQACL
                     NEYGLVGEDMFRAALAMIHSALNADLKGIGRLCKVKHKVPEFRFLARAQGFSGDKNLL
                     VRDGYIHGAIDLVFRVKDTYYILDWKTNTLPDYSESSLQTSIAEERYDLQYSIYGHAL
                     ISWLNSSGKFEPSRFGGVFYLYLRGMKPGETDGVYFVRPDPAALEDLAGIIRRFSAGE
                     PLV"
BASE COUNT         2476 a         2575 c         2504 g         2256 t
ORIGIN      
        1 cagaaggaat tccgttcatt cctcgaaaag aaatccagag cacgcgcgaa gacggcgaat
       61 ctttcactca gcacgaaatg gatctggact cgattttgag acgaaagccc gcgattgttc
      121 tcgtcgatga acttgcacat acgaatgcac ctggctctta tcaccccaag cgatacatgg
      181 atgtggaaga tttgctcgag ggcggaattc atgtctacac gacgctcaat gtccaacacc
      241 tggaaagtgt agccgatttt gtggaacaga tcactggagc cgccgtgcag gagagagtgc
      301 ccgatcgcat tctggatctg gcaaacgaaa ttgaactgat tgatataagt cctgacgaac
      361 ttcgaaaacg gctccacgaa ggcaaagttt acagaagtga tcgcgcggaa gttgccgcgc
      421 gaaatttctt taaaccggag aatctgctcg ctttgcgcga gatggcgttg cagaccaccg
      481 cgcgcgtagt ggatacagaa tcgctcaagc gaagaacatc cggagaagtg ctacgaacgc
      541 gcgaacgaat tctggtcctg gttggcacgg atcgcttcgc ggcgcgtctg attcgaattg
      601 gacgcagaat ggccgcagat ttgaaggcgc cgtggttcgt aatgcatgtt cgctcctctc
      661 atagtcgggg agaagacaat tccaggatcg aatcggaatt caatctagcg cgctcgctgg
      721 gcgcggaagt acttctcatg gatggagatc tggtagacgc cgtggaacag gtgatcgccg
      781 aacgcagcat tacgcaaatg ctcgtgggaa gaacagaaca tcgcttttcc ttttcgcgac
      841 ccatcacgca tagactgatt gatcgcgttc gcaatgcaga cattcacatt gttccgataa
      901 aaagagaaat caacgctcgc aaaagaagat cctacagact ccctgaactc cgctctccct
      961 tcaaggaata cgcagttgca agtggggtca tatggacttt gacagtcgga tgcctggttg
     1021 ccttccctga tggacagtat tccactccat cgatcatctt tatagcggcc atcgcagccc
     1081 tggggctggt gtttggtcgt ggacccgtgc tgtcagccgc cgttcagtct gcgttcgcct
     1141 gggattttct ctttattcct ccccgttata cgctcacgat cggaaaactc gaagatcgac
     1201 tcatgttcgg attgttcttc gcagttgcca ttgtgacggg cattctaacc agtcgccttc
     1261 gcaggagtaa ggcggacctg ctgcgaagac agagggacct ggtgcaaatc caggaaatat
     1321 cgcaggcact cgcaggcgca accggcgttg aggaaatcac gggtcttgtt tgcaataaac
     1381 tgagcgccat ttttgatatc ccggactgca tgatattgct caaacaaggc aagatatcta
     1441 ccatgttgcc tgcgactgca agcatcgatg ccaaagagca ggcagtcgcg gactgggtag
     1501 tggagaatgg caaaccagct ggaaaaggca cggatacttt accgctttcc gccgcactct
     1561 ggattcctct gaagactcct tcgagaggca ccatgggagc acttcgtctg cctgtggaaa
     1621 atccggacat caatgagctg aatcttgtcg gtgcaatagc agcacaggcg tctcttgcgc
     1681 tagaacgcga actcctggcc tcaagcgctc gccaggcggc ggtcaatgaa gaatccgaga
     1741 aattgtttca tgccatcctg agatcgattt cgcatgaact tcgttctcca gtgactgcta
     1801 tgcttgctgc agcggaaaat ctctcgagag accgggtcat gatggacgat ctcttgcgaa
     1861 atgaagtccg tcaggaaatt tcagattctt cacagaggct tgcccgcgtg gtggatcagt
     1921 tgctcgcgat gagcagaatc gagagtggtc gcctgacgtt gcaattggaa aaccacgatc
     1981 tgcgtgacct ccttcaagaa tgcatggctc aattgggcaa agagttcgca ggtcacacag
     2041 tcaaaatcga tgcgagcgaa aaggtggagg ccaggtttga ttttccgatg atgcgacagg
     2101 ctttgattgg agtactccac aatgcggctc tccacacacc gatccacaca atcgtccaga
     2161 tctggatttt cagagaacga gcatctgcag tgatcatcat agaagacaat ggacctggaa
     2221 tcccaggaga tccaggaaag ctatttgagc catttttccg aggtgcggcg gctaagcccg
     2281 gtggactcgg tcttggcctt tccattgcga aatcagtggt cgagctacat ggaggaagca
     2341 ttgtagcaac cagaacagac acggggagtc gctttcgcat cgaactgccg ctggaaaaat
     2401 catgaaagag aaaattcttt tgatcgaaga cgaaaagcca atccgcaaaa tgctttcgat
     2461 caatcttgaa tcagaagggt atacagtact cgaagcggac tcaggaaagg aaggtgtgca
     2521 ggtttgcgca agaaatctac ctgatcttgt gttgctcgat ctgatgcttc cggacatgca
     2581 agggcaggaa gttcttcgca tgttaagaga attcactgtt gtccctgtga tcgtactttc
     2641 tgcagtctca tctgaaaaaa gcaagatcga actactcgat cggggcgctg acgattatct
     2701 cacaaaacct ttcagcccgg gagaactcct ggctcgcgtc cgcgttgggc taagacatgc
     2761 gcagtccagc aagacagaat ctgtcctgga agctggtcca attcgtctcg atccgtctga
     2821 tcgcaccgtt cagatacgcg gaaaccagat tcatcttact ccaacggaat ttgcaatcct
     2881 ggcacttctg cttcaatacg ctggaaaaac ggtgacgcgt gaaatcctaa tccaaacaat
     2941 ctggggaagc aacgaaaacg aaacaggaag tctgcgcgtt catatcaacc agcttcgcaa
     3001 gaagatcgaa accagtgcag ccatgcccga gctaattcta aacgaaccag gagttggtta
     3061 cagacttgcc gtgtaacgtt attctcaatg acccatttat tcgcgcggct cctggatctc
     3121 actgccgtcc tgcgaaacca tagagcgaaa acttgtgtca tttaaaccaa gccagacggt
     3181 ctggccaacc tccgttgccc actcgttgga aagcactttc actttgatga tgtctatttc
     3241 gaattcattc ttataaattc ttcgatccag aagctcaagc tccgccccgc gagaaacacg
     3301 accaatgtac gcattcgaat catagccaga atctcgccca taaaccgcgt ctccgaattt
     3361 gttttcctcg cgaccaagga ttctctttgt tgaggcaatc ggcggcatgc cgaactttgg
     3421 atcgcgatag ttgtattctt tcttttcctc ttctggattg gcttccggct tagcagactc
     3481 atcttctgga atagagagct gcggctcttc tgcattctgc tgagagcgaa actgactcac
     3541 agtagaagga acgccgcctg acgttccact caagtccttg atcttttctt ttaatttctc
     3601 gttgcgccag taaaaatatc cggcaatcag tatggccact acgattccaa tttgatagaa
     3661 ttcggaatgc attcagaaac ctggttcgcg ccggtgatct gtcgacttat tttctaaggg
     3721 cgtattcgcg ctcgagccgc gcaagcattt ccccaacacg cggatcgcct tcgcgcggag
     3781 cccacgaagc aaactcttta tcgtcacttg gacggtacgg tccgggcttg acttcaaagc
     3841 aaacgcacct ttccgacaag acaagaagac catgccagat gcccggagca atgtcaattc
     3901 cgcgaacagg accatccgcg ctcaaggtgt ggatttgttg gaccactcct gagtcatcaa
     3961 aaatgaagaa cgcaatgctc ccttgcagaa tcaaaaagct ctccgctttc ggaggaaatt
     4021 ggtggcgatg cgggcacaaa taggttccct tcaatagcac gttgagaaaa cgatgcgggt
     4081 tgtcgtccat ggagtcatgg aaattatggt tgatgcgaag gcgcggcttt gctctggcct
     4141 cggctgccag cgaatcgaac atctcttcgc gaatcaattg aataggttca ctcatgtcag
     4201 aagtcctcaa gtcggaagaa aatccatacc gcacacagag cgcaagcctg gaatcagacg
     4261 gcagaacggt ctacctctat ctaaatcccg tggaaggtgt ggacctggtc ccgcgcgccg
     4321 tgtgggtgat gaacctggtg cccgctcctg cagaagacgc ggacggaatg atccgcggcc
     4381 aggcacctgt cagaagtcgt gctgcgtgcg cgcatccgaa tggaatgaaa gctccgcatg
     4441 aaaacgaaat agacttcgtc tggtttcagg aaggcacggg cgtcactctc tgtgtcgaag
     4501 gtgaaatctg ggcagtcatt ccgccctttt ccggttcaga tggattctac ggattttgtc
     4561 gcgaagcaac tctgcccgag cacggcacgt tcccgcttcc ggatggaatg aaagcgaggg
     4621 caaaggagaa cctggatttc tggaaccgtc gcacgcagaa aggtttctgg gcacagtttc
     4681 aggggaatct actcagaacc tatgagtctg cattcggacc gcataacaac tattacgtgc
     4741 tagacaacgg caagtttcca gccatgggtc tggctgagtt tatcattcca gacacaaccc
     4801 ttcgcctctt tgccacgatt ggaatgtccg cgcaaaatat gcctggtgta gaattgcatg
     4861 tcaaaaatcc agagccgctg tttcgtgtgg aactactgac cgttcgcgat gcgccccatc
     4921 cctggttcat agatagcatg tctcgcgttg cactccttcc ctggagaaca ggtggctggg
     4981 ttggtccggg ccattccatt caatttggca ggtcggagcc catgtctgac tatctggtcc
     5041 tcgaacacca cctggtacag gacaagattc ttgctgattt gtttgcgaag ctaccatctg
     5101 tattcctcga tgaacgttat gcgcgaacct tcctgtttct atggccggcc gtaccagacg
     5161 attttcgcgt caaccaggca agggggagag aatttctcgc gaagaaaaac agcgtagttg
     5221 agcgtttttc atgatttctc ttgatccacg cttcgaagaa gatttcaaaa aaattcgcga
     5281 ctccggtttt gaaccaggcc gggtggtttc tcaaacggtc acctacgaag tagcaacaga
     5341 atcgggtgat tacacagcgc aacccgcagg aaaactcctc ttttcaggaa tgcttccagc
     5401 agtgggcgac tgggtggcgc tacaggttta cgagggagga gccatcattc atgatatagt
     5461 tcctcgcaaa accgtgatta tgcgaaaatc tgttgggaaa tcctccgctg accaggtgat
     5521 tgcggccaat ctggactacg ccgtcgtggt gatgggcctg gactcaaact tcaatctgcg
     5581 aagactggaa cgttatacga ccatcattgc cgcctcaggc gcgaagggaa tcatagttct
     5641 gagcaaggca gatctcgcag atccagagga aatcaaactg aaagtcgacc acaccattgc
     5701 atgcgcaccc ggattgcccg tcatcgtatg gagcgatgca gacaaacgcg gcctggaaga
     5761 actgatggca attctgcgtg ggcccgcaac tttctgcctc cttggctcaa gtggcgcagg
     5821 caagtctaca ctcatcaatg caatcgcagg caaacccatc gcaagaaccg gagctgtccg
     5881 cacatctgac gaaaaaggca aacacacaac cacggtgcga actctcattt cgcttggaaa
     5941 tggaatcgac ttgatagaca accctggcat gcgcgaaatt ggactcacct ctgaagaggg
     6001 attatcggat gcttttccgg aaatcactca ggccgccgca gaatgcaaat tccaggactg
     6061 cacgcacgaa aacgaacctg gatgcgctgt ggaactcgcg gtacaggagg gcagaatcac
     6121 tgcagaacga ttcgcctcct atatgagact tcttcacgaa tctcgcacta tagagtctat
     6181 gaaaagtgag cgagggcgtc agaagaaaaa gcaaaacgaa aaaactcttg cgaaaatgat
     6241 tcgagcgcgt ctcaaggata agggtcgttc atagaatcca ggctgagtcc gcctgtttcc
     6301 cgacgtggat tgcgctggat gcccgcgcga agaacgcttt cctttccata aatccacaca
     6361 agctctttgg cgcgagtgag tgcggtgtag aggatttgcc tgctgaccat gggatggtct
     6421 gattccggaa gccatagcaa caccctgttg tattcagaac cctgagattt gtggaccgtt
     6481 gtaacaaaag caggttcaca ttcaggaagg gattcgggag gtagtagtct gatttcctct
     6541 ccacgggcgg caatccagcg aaaaccggtg cgaaatcgca cgaggagtcc agtctctcca
     6601 ttgaatagtt cgcgcgaata gtcgtttcga gtgatcatca gaggaagtcc ggaaaaccac
     6661 tctccgaaca ttacgcttct gttttgcttg aggaaaagtt tcatcatcct ccgattgagc
     6721 cgcacagacc cgtcttcact ctgtcgtagt gcactgagga tgcgcgcgcg acctgttact
     6781 tcagtcaaat gccgcataac gtctacggct gcaggatcat ccgcatccag agcagaagcc
     6841 tctctcaaga gatctacaaa ctcaggtcca tacgaaagct tgagccaggc gcccagcact
     6901 tcattctggc cgccgtcccg aaatgcaatc tgaactaccc cgtgattctc gggcaattca
     6961 cggagaggaa caattgacca cggtaaactc tcctccgctt ttcgcacagc atccgaggcg
     7021 cgaattacaa cgtcagccga gcggtgactg gccgtcaggt gaaccatccg gtccaccgcg
     7081 cgactttttc ctggcatttg cggcaaatcc aactcaggat ccagggtacg cagccacgac
     7141 gcgaagtctg gactgtattg ggggttgccg ctcagtgcga agagttccac aagatcaccg
     7201 agcacagcgc ccgcgtccac agacggaagt tgattgcgat ccccgatcag aatcacgcgc
     7261 gttcgcgttg gatccaccgc tgcaagcaat cgcgacatga ggtccacgtc caccatggag
     7321 acttcatcca ctaccagaac atctccgatc aatttgcgat tcggtccaaa atagaaatct
     7381 tgagaatccc tcctgtaaga caacgcagtg tgaatcgtgc tggccgtgta ctggtccccc
     7441 agtgtctcag ccattcggcg cgcagcacga ccggtgggtg cgcaaagaat cacacgatcc
     7501 gctggaatgc ccgcgcgaag gaatgaatcg aggactgcac gaatcacggt tgtttttcct
     7561 gtgcccggcc cgcccgtaat gattacaaaa ctactaaaga gcgacagggc caccgccagc
     7621 ttctgttcag cactcagagt caggccagac accgacaggg cagtgccata cgcctctttc
     7681 gctttctcga gagggcgcgg aggctgcgcc gccatgcgtg cgaaatctct ctcgatgttt
     7741 ctacgaagta agaaatactt ttgaaaaaat agaaatgtat cagagaggac caccgggctt
     7801 ttttcgtctt gtccgatcaa tggcgaataa cggtttatcg atttcgcagc cacaatcgca
     7861 tcttccagat ttggctccgt cgtgtcatgt ctttgaacaa aacgatcccg cgttgaagtg
     7921 agcgaaagat catccaatcg catacacaga tctccgtgca tgcatgtgtc cgcgagaaga
     7981 agaagcaagg ctcgaattga ttcatcccct ggaaaatggg agaactcgag cagatcatcc
     8041 acaagagaaa cccagaggta gagctccgga ttttctctgg ccgcttcgcg aacggttcta
     8101 ctcataccag gggctcccct gcagagaaac gacgaatgat gccggcaaga tcctctagag
     8161 cggccggatc tgggcgcaca aaataaaccc catctgtttc cccaggcttc atgccacgca
     8221 agtagagata gaacacaccg ccaaaccgcg agggttcgaa ttttccgctg gagttcagcc
     8281 acgaaatcaa tgcatgaccg taaatagaat actgtagatc atatcgctct tctgctatgg
     8341 acgtctgcaa agacgattcg ctgtagtcgg gaagagtatt tgttttccaa tccaatatgt
     8401 agtacgtatc tttcacccgg aagacaaggt ctatcgcacc atgaatgtat ccatcgcgaa
     8461 ccagaagatt cttatctccg gaaaaacctt gagcgcgtgc gagaaatcga aattcgggga
     8521 ctttgtgctt cactttgcat aatcggccaa ttccctttaa atccgcgttg agtgctgaat
     8581 gaatcatggc gagcgccgcg cgaaacatgt cttctcctac gagtccgtat tcgttcaggc
     8641 acgcctgaat gtgatcgcgt gttgctggct caagggtgcc cggttttggt acttgcttcc
     8701 tgtcaaattc agaaaaatcc agaccctcaa gtgcctcgtg gatcgcattg cccgtcgctt
     8761 ctccccgcgg aagctccgga tgatccacga tcaggaaatt acctttttga tcgagcgttg
     8821 gatcgcgcgt ctccggaaga ggaagaggtt catcttccgc cgggtttgta tctccaaact
     8881 cagcacgaag cgcatccaga gcaacagctc gcctgagaat cccagagtaa gacgtgagcc
     8941 ccgctgcacg cacagatagg ttggtaggaa agcggctcaa ttctgttctt cccggtgctt
     9001 tggcggttcg ggtcagtggc tcaggaggac ctgcgtgcat gggctgaacc aatcgcgcca
     9061 ggttttgcgc aagaacctga tccgcggcag gcgctacaag gtgagacaat ggaccgcctc
     9121 ttccactggg aaagaagggg gcaaacaccc tgtctcgcgc tcttgtaaaa gctacgtagt
     9181 acaatctttt atattcttca aacctctgga ttttgaagac gtctcttgtg gaggccgcca
     9241 gatcaaagat tctcgcaccg cttccgccag ggtcaaagta atccagatac gcctgtccgt
     9301 tgaattcgcc aaatccacct gcaatgaaaa cacagtcgaa gtcgaggcct ttgctcgcat
     9361 gaatcgtcat gattcgcaca cgatccagat ctgattcctc gtgatgcaga tccgattctt
     9421 cgtccgcggt tgtttgatcg ttgatccttg cgcgtaatgt tgccagcgcc tcagacagag
     9481 tggtgcctct gcagagaatt tcaatgtccc tggcaatgtg ttccagattc gcgcgccgac
     9541 gcgggtagtc atccacaggg tcgagctgct caccagctaa tacgttctct gcatttccag
     9601 gaagaagagc gagagtgtcc accatcatgc ttcgcagaag acgtgcgatc ttcccttcga
     9661 gagccagagt attccattga cgaaattgag agagtacgcg ctccggaacg tccgaattca
     9721 caagatcaat tggctggacg cgaaagaaat tggtgagcat tgcttctttg atgtcatcca
     9781 tgcgaagagt gaaacatgcg accagcatca g
//
LOCUS       DAOQJL010000083         9655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3735_length_9655_cov_5.025104, whole genome shotgun sequence.
ACCESSION   DAOQJL010000083 DAOQJL010000000
VERSION     DAOQJL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_9655_cov_5.025104"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLG78_05460"
     CDS             <1..498
                     /locus_tag="PLG78_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22590.1"
                     /translation="EGMNLLFWAGTPTHPTMRGPCLIPIIPEDQPILDTYDLRLHMRI
                     LDAGKLNADVNLLSDSLAALSPAGVQKPRGITEYNFHQGVDSILEYYGADSSWPRAFK
                     INSDRTYEIWLPGIIYAQTDHFDFAPAFLIRGEIKKMPAIRFEPALRRQYTPYYFPFT
                     YAPVR"
     gene            529..1659
                     /locus_tag="PLG78_05465"
     CDS             529..1659
                     /locus_tag="PLG78_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE22591.1"
                     /translation="MEEASLYISVISLAFLAFLSILLLNELRTGAGNFYLTGVVFIAA
                     LASLLRFSVRTANPLLLILPFLVFPSGFLVGPLLYLYARHTLYGSPPGRFEIYAFSAV
                     TLFVFASHTVLSSVFPEMRSMSDVQLQVGFVTVYTRSLIFTSCIYNGAFIVRALLLVS
                     RYEAEVAQHFAGSVRDQVLWLKSLFGSTLLFFVLYFTSTVAAWIAGFSLPATPAEGIV
                     IVAMAYLVLFYYIKKPQVFALRMEQTSNVRYARQNLADADRLEHMRRLEEYMVREKPY
                     LDQRISLSDISESLSIPVHHLSMVINIEKKQNFFGFINSYRIAAAKAILSSAEFAERT
                     LLDAGFMSGFQSKAAFNRVFKEHTGMTPGQYRAFAQKKITSP"
     gene            1740..2165
                     /locus_tag="PLG78_05470"
     CDS             1740..2165
                     /locus_tag="PLG78_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22592.1"
                     /translation="MNEKLFLPVRILLTLGVAEFFGPALRDSGVSHLQNPEWPGHAKV
                     HMMWFIAYLFASGLSQIYLIWFRRPFDRGNLSLTFIWQFCNLFAFWAAVFLAPVYGGA
                     VIDGKYHTQIFGINENILAFGFFSLLWLTGLFIFRARSR"
     gene            2168..3922
                     /locus_tag="PLG78_05475"
     CDS             2168..3922
                     /locus_tag="PLG78_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNE22593.1"
                     /translation="MLKTDVLIVGGGPAGLVSSLCLATLGIRSIVVEKRPHHATHPKA
                     HEISARTLEILESIGIPIRELAKEASPHEDASRIVFCRTMAEEIGRIDLNQPDIQKKY
                     LNHVSVSRPYLNLSQTELEKILRKHVAENQMVQLLTGLEWVSLTQSPHNVISALRTTD
                     GKMNQIQSKYLLGCEGAGGAVRRSLGIKMEGPDQIQDFANAFFTNDLSKIAPRAKLFF
                     VFRPEAAGTLIAHHSRKRWVYHVPLLPHQKAADCDADFFRKRIRLALDIPDFEPNIES
                     ISSWRMTAQVAARFRKDRVFLVGDSAHRFPPTGGLGLNSGVADAHNISWKIAHVLKGE
                     NDRILETYEEERKPVVAFNCEESRRNYFNLLSIPAALGLHKFALDWGARILYSFPLRW
                     LGKSREPILACAMRFADRRLKIALTDKNSRRVTGARLEIHNQISHFDRIGLDLGYRYS
                     PDALLPDQIRFPDSTSTEYRPSFAPGARLPHFEVPGRGHASIHTRLRYDRWSLLCSGE
                     NASWSRAIRNAGLHRKIQVIPILNEVRMGDQTLSFAALAKMEEGGGLLIRPDGHVAAR
                     MRSRDDLYQTLNRFFSVP"
     gene            3963..5021
                     /locus_tag="PLG78_05480"
     CDS             3963..5021
                     /locus_tag="PLG78_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22594.1"
                     /translation="MRYSFFLLFLSLAVAVHAAPSITPESMALYHAGHSAAVSGDRAA
                     ARGKFDAALARDPKNFLAMFDIGGLAEDAGRKEEAIEAYEKTTAIAPDFAEAQVELGT
                     LYLNVQRNPDKAIARFRIALLTKVPYADSRFALSHTRGQALRNLSVSYAVKRQFGLAQ
                     GIARSFLSDSGVNHEGDAAMNTLLQRSSGSMTAVTTRKFAEDLEPISKQLHGGKVAEA
                     LSAYLAFEKSHPLASLPPMDAWDLYEGIGLAYAMTNRNAEALVAFEKAVEAGMPLPYG
                     KLQEAQFNTACALALLGRAEEAIARLEEVLWEDQITTVDPERTRKNGYAPLIKKDETL
                     ASIRQHPGYLKLFETYGP"
     gene            5064..5486
                     /locus_tag="PLG78_05485"
     CDS             5064..5486
                     /locus_tag="PLG78_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22595.1"
                     /translation="MKRLFLALIVLSPFAIHASAVRECKVNATITRIMRDGKSPSFEI
                     HATRAAFERGHSMGEDCSELLMFEGNHVVELKDLSSPFKLQVGAPIILLFVEVRGVTK
                     DGPQMSRHWEVYPENPKSSCDENAYMVTRPKVRILERF"
     gene            complement(5449..6378)
                     /locus_tag="PLG78_05490"
     CDS             complement(5449..6378)
                     /locus_tag="PLG78_05490"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE22596.1"
                     /translation="MRRVVILNPRSKNGASLKAFNKLKPDLHKLLGPFEVYQTKAPRD
                     ATEQVRSILKQGSFDQILIAGGDGSINEAVNGYFHEGKLLRADVPLGVINLGTGGDFY
                     KTLLRDSSNYQEALSQNRLRMVDCGLTTLEKGLDPQYFINITSIGLGGDVNRQMKASS
                     FQSGMPAYFYHSLTSLLKYSPPNCKFIMKDKEGKTTEIETGLVNFFVCNAEFNGGGMR
                     WAPESDIEDGVFDLVLVSNVPKHKLITESHKIYSGKIREMTGVQEFHASEVIALPERT
                     VSQEIDGETRDMDHLRTHEFHFKIVPRSLPLVL"
     gene            6495..7760
                     /locus_tag="PLG78_05495"
     CDS             6495..7760
                     /locus_tag="PLG78_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE22597.1"
                     /translation="MRNIAVLSDDADLNAKIASSCNSFGDEFQAVFFHEERAFVQYLN
                     YELPEIDIIDFSGSKASAGRAFTEIKDDPWLHFGGSIIIYEKQKEEDLLNRLRGVNII
                     AHIQKSKVELYLPRALNVLSQHRSILFQRDLHALLQSNISGMFVLDNDPFDVTTYSNL
                     IANFLYNSNLINLDQKSNCSAALVELLYNAIEHGNCCIGFEEKKVWLENGGDILDLIR
                     QKAMKPEILAKKIFLNYRITPERSYFTVRDEGEGFDWGSRLAEDNPAEALHGRGIMIA
                     RNYLQNVHYNEKGNEVSFQIEHLHESNVIPRVFADQEEVTVQDGEVIFRQGETSSHLY
                     YIISGKYDIIANGRKVSTLTPADIFLGEMSFLLNNKRSATVIAVGRGQLMKISKQAFI
                     NAIREHPHYGIFLARLLAQRLDQVHEVGL"
     gene            7776..9095
                     /locus_tag="PLG78_05500"
     CDS             7776..9095
                     /locus_tag="PLG78_05500"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22598.1"
                     /translation="MIRTVLRGFRRVSAVILGRPDFANLDQRLFNVMCFGGTIVAVLS
                     AIENAFIDVPFVLVVGSCVQTVILASGFVVSRYWNIYRPIVWPVILAILVFLAMQWFW
                     NAGSVGGAHYFFFVCGMMGAVIMRGWQRYFVILLYLVVINGVLYLEYIRPDLIVNYSD
                     RQSRYVDIAFSLSLCMILFGLVIAIFSGHYQVLVNRVQRHKLEFVEDLHLARILQETV
                     FQHDRTLTQGFDCEIVYRPSAALGGDLYDLSRRLGILRVFMADMKGHGVNAALSSMLI
                     KSEWVHSGHASFSAGDALANLNKQVVARYGDSLVLSAIVADLYEDSIHFAAAGHVPQF
                     RVFGGEVLRLEATGVPIGVMEDSQYSTERTALPRGSRLVFFTDGFHDQPDLSGKTIGT
                     DWMEEILRKSQPSSADMVRALISKFHAKTGTEPGTTADDLTLIVIGR"
     gene            complement(9099..>9655)
                     /locus_tag="PLG78_05505"
     CDS             complement(9099..>9655)
                     /locus_tag="PLG78_05505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNE22599.1"
                     /translation="FLLQAAPWIRQKLQANQERPSAARLRYVHGEKIPWQGKEILLHV
                     LPGIRQSRLTDESLTVHARNALIAKKKVAAVYSKHGEKIFRETVSRFAPIVGKHPSRI
                     SVRGMKSRWGSCSHTGSLSLNWKILAAPQYVIDYLIVHELCHLIHANHSSRFWAEVER
                     VLPEYKEGRKFLKDHGSSILIRFD"
BASE COUNT         2236 a         2345 c         2492 g         2582 t
ORIGIN      
        1 gaagggatga accttctgtt ctgggccggg actcccactc atccgaccat gcgcggaccc
       61 tgcctgattc cgatcattcc agaagaccag ccaatcctgg atacgtacga tttgcgactg
      121 cacatgcgca ttctggacgc aggcaagttg aatgcagacg taaacctact ctcagacagt
      181 cttgccgcgc tttcgcccgc tggtgtgcaa aagccgcgag gcataacgga gtacaatttt
      241 catcagggcg tagattcaat tctagaatat tacggtgcgg attcatcctg gccgcgagca
      301 ttcaagatca attctgacag gacgtatgaa atctggcttc caggcatcat ctacgcgcag
      361 acagaccatt tcgactttgc gcctgctttt ttgattcgcg gagagataaa gaaaatgcca
      421 gccatccgat ttgaaccggc tcttcgcagg caatacactc cgtattactt tccctttaca
      481 tacgcgccgg ttcgatgatt ctgttcgcag tcttttcgcg agtttcgttt ggaagaagca
      541 tccctatata tttcggtgat cagtcttgcg tttcttgcgt ttttgagcat cttgctctta
      601 aacgaattga ggacgggtgc cgggaatttc tatctcacgg gtgtcgtctt cattgccgca
      661 ctggcgagtc tgctgcgttt cagtgttcgc actgccaatc cacttcttct cattctgccg
      721 tttcttgtct ttccctctgg ttttctggtg ggccctcttc tctatctgta cgcgcgtcac
      781 actctctacg gatcgccgcc aggcagattt gaaatctacg cgttcagtgc cgtgactctg
      841 ttcgtatttg cgtctcatac tgttttgagt tctgtctttc cagaaatgcg aagcatgagt
      901 gatgtacaat tgcaggttgg ctttgttaca gtatacacac gatcattgat ctttacttcc
      961 tgcatttaca acggcgcatt catcgtgcgt gcactcctgc ttgtttcgcg atatgaagct
     1021 gaggtggcac agcattttgc tggaagtgta cgtgatcagg ttctctggct gaagagtctt
     1081 tttggcagca cgctcttgtt ttttgttttg tatttcacaa gcacggtcgc ggcctggatt
     1141 gccggatttt ctttgcccgc gacaccggcg gaagggatcg tcattgtagc aatggcttac
     1201 ctggtattgt tttactatat aaagaagcca caggtgtttg ctctgcggat ggaacagacc
     1261 tcgaacgtgc gatacgcgag gcaaaacctg gcagatgcgg atcgtctcga gcacatgcga
     1321 aggctagaag aatacatggt aagagagaag ccctacctcg atcagaggat ttccctgtcg
     1381 gatatttctg aatccctctc tattcctgtt caccatctct ctatggtgat caatatcgaa
     1441 aaaaagcaaa acttctttgg ctttatcaac agctatcgga ttgcagctgc aaaggcgatc
     1501 ctttcgtctg cggaatttgc ggagcgcacg cttctggatg ccgggttcat gtccggcttt
     1561 caatccaagg ctgcattcaa tcgcgttttc aaagagcaca cgggaatgac tccagggcag
     1621 taccgcgctt tcgctcaaaa aaaaatcaca tcaccctgaa ctttttgagt ctcagcttgc
     1681 aagcggagtc gactcgattt tctggatgcg ctatgatgca gcatacggag tgatcaaaca
     1741 tgaacgaaaa gctatttcta cctgtgagga ttctacttac gctcggcgtt gcggagtttt
     1801 ttggccctgc gctgcgtgat tccggagtat ctcaccttca gaaccccgaa tggccgggtc
     1861 atgcgaaggt acacatgatg tggttcatcg catacttgtt tgcatcaggc ctttcgcaaa
     1921 tctacttgat ctggttcagg cgaccttttg atcgcggcaa tctttccctc acatttatct
     1981 ggcaattctg caatttgttt gctttctggg ccgccgtctt cctggcaccg gtttatgggg
     2041 gggccgtcat agacggaaag tatcacactc agattttcgg aatcaacgaa aacatcctcg
     2101 catttggttt cttctccttg ctctggctta cagggctgtt catttttcgc gcgcgcagtc
     2161 gctaaggatg ttgaagacag acgtactcat cgtaggtgga gggccggccg ggctggtcag
     2221 ctctctgtgc cttgcaacgc ttggaatcag gagcatcgtg gtggagaaaa gaccccacca
     2281 cgcaactcac ccgaaggcgc atgaaatcag tgcgcgtact cttgagattc tagaatccat
     2341 tggaattccg atcagagagc ttgcaaagga agcttcgccg cacgaagacg catctcgcat
     2401 tgtattttgt agaacaatgg cggaagaaat tggccggatc gatctgaacc aaccggacat
     2461 ccagaagaag tatctgaacc atgtgagcgt ttcgcgtccc tatttgaatt tgtctcagac
     2521 tgaactggaa aaaatccttc gaaagcatgt ggcggagaat caaatggtgc aacttcttac
     2581 cggtctcgaa tgggtcagtc tgactcaatc gccgcataac gttatatccg ctcttcgcac
     2641 gacagatggc aaaatgaatc aaattcaaag caaatactta ctgggctgtg aaggcgcggg
     2701 tggcgcggtg agaagaagtc tgggaattaa aatggaaggg cccgatcaaa tccaggattt
     2761 tgcaaatgcg ttttttacca atgatttatc aaagatcgca ccgcgagcaa aactgttctt
     2821 cgtattcaga cccgaagccg ccggaaccct cattgctcat cattcgcgaa agagatgggt
     2881 gtatcacgtt cctcttctgc cacaccagaa agccgcggat tgcgacgcag acttctttcg
     2941 caaacgaatt cgactcgctc tggacattcc tgatttcgaa ccgaacatag aatccatcag
     3001 ttcctggagg atgaccgcac aggtcgccgc gcgttttaga aaggacagag tgtttcttgt
     3061 gggggacagc gcgcatcgat ttccgcctac gggtggtctg ggattgaatt ccggagtggc
     3121 ggatgctcac aatatttcat ggaagatcgc tcatgtgctc aagggagaaa acgatcgaat
     3181 tctagaaacg tacgaagaag agcgtaagcc agtggtagca ttcaactgcg aagaaagtcg
     3241 tcgcaactat ttcaatttgc tctctatacc ggctgcgctg ggcctccaca agtttgccct
     3301 cgattggggc gcacgcattt tgtacagctt tccactccgg tggctcggaa aaagccggga
     3361 gcctattctt gcctgcgcga tgcggttcgc tgatcggcgt ctgaaaatcg cgcttactga
     3421 caagaacagc aggcgcgtga ctggagctcg cctcgaaatc cacaaccaga tttcccactt
     3481 tgatcgcatt ggactggatc tgggttatag gtacagcccc gatgctctgt tgccggatca
     3541 aattcgattt ccggattcta cttcaactga gtacagacct tccttcgcac ccggagcccg
     3601 tctgcctcac tttgaagttc cgggtcgggg ccatgcgtcg attcatacaa ggttaaggta
     3661 tgatcgctgg agcctgttgt gttctggcga aaatgcttcg tggtcgcgtg cgattcggaa
     3721 cgcgggactc catcgaaaaa ttcaggtcat tcctattctg aatgaagtgc gcatgggcga
     3781 tcagactctt tctttcgcgg ctctcgcaaa gatggaggag ggcggaggct tgctcatcag
     3841 gccagacggt cacgtagcgg cccgtatgcg atcgagagac gatttgtacc agacactgaa
     3901 cagattcttc agtgtgccat aattttggct atgaatccgg gaaggtcgtt tcatagttta
     3961 gcatgcgcta cagtttcttt cttttattct tgtcgcttgc agttgcggtg cacgcggcac
     4021 ccagcatcac ccctgaatcc atggcattgt accacgccgg tcacagtgct gccgtttctg
     4081 gagatcgtgc cgcggctcgc ggcaaattcg atgctgctct ggcgcgggat cctaagaatt
     4141 ttctggctat gtttgatatt ggagggcttg cagaagacgc gggccgcaaa gaagaggcca
     4201 tagaagccta cgaaaaaaca accgccatcg cgccggattt cgcagaagct caggtggagc
     4261 tgggaactct ttatctcaat gttcagagaa acccagacaa agcgatcgca aggtttcgca
     4321 tcgccctttt gaccaaagtt ccttacgcgg attccagatt cgctctttcg catacgcgtg
     4381 gtcaggcgct acgcaactta tccgtcagct atgccgttaa gcggcaattt ggactggcgc
     4441 aggggattgc ccggagcttc ctttctgatt ccggagtcaa tcacgagggg gatgccgcta
     4501 tgaatactct tctccagagg tcctcaggat ccatgactgc ggtcaccacg aggaaattcg
     4561 cggaagacct tgagccaata tcaaagcagc tacacggagg aaaggtcgca gaggcgctca
     4621 gtgcatatct tgcttttgaa aaatcgcacc cgctagccag cttgccgccc atggatgcct
     4681 gggatcttta cgaaggcatt ggtcttgctt atgcgatgac caatcgcaat gcggaagccc
     4741 tggtagcttt tgaaaaagcg gtagaagcgg gcatgccgct cccatatgga aagctacagg
     4801 aagcgcagtt caatacagct tgcgctcttg cgcttctggg ccgggcagag gaagctatcg
     4861 cacgtctgga agaagttctc tgggaagatc aaattaccac tgtggatccg gagcgcacgc
     4921 gtaagaatgg ctatgcgccc ctcatcaaaa aagacgaaac tctggcttcc attcggcagc
     4981 atccgggtta tttgaagttg ttcgaaacat acggaccctg aaaaaacgga aaacaaatcc
     5041 attcatcgtt gcgtcggatg agcatgaaaa ggttgtttct agccctcatc gtgctcagtc
     5101 catttgcgat tcatgcctct gccgtgcgcg aatgcaaggt aaatgcgacg attaccagga
     5161 tcatgcggga tggaaaatcg ccttcatttg agatccatgc gactcgtgcc gcgttcgaac
     5221 gcggacatag catgggggaa gattgcagtg aacttttgat gttcgaaggc aatcacgtag
     5281 tcgaactcaa agacctttca tctcctttca agctccaggt tggcgctccg atcattctac
     5341 tgtttgttga agttcgaggt gttacgaaag acggcccaca gatgagcagg cattgggaag
     5401 tctatcccga aaatcctaaa tcatcttgcg atgagaacgc gtatatggtt acaagaccaa
     5461 aggtaaggat cttggaacga ttttgaaatg gaattcgtgc gttcgcaaat gatccatgtc
     5521 acgcgtttcc ccgtctatct cttgcgatac agttctctcc ggtagagcga tcacttccga
     5581 tgcgtggaac tcctgcacgc ctgtcatttc gcgaatcttg ccgctataga ttttgtggga
     5641 ttctgtgatg agtttatgct tgggcacgtt ggatacaaga accaggtcaa acactccgtc
     5701 ttctatgtca gactctggag cccatctcat gccaccgccg ttgaactctg cgttgcacac
     5761 aaagaaattc acaagccctg tttcaatctc agtagtcttc ccttctttat ccttcatgat
     5821 aaacttgcag ttaggcggtg agtatttcaa cagacttgtg agcgaatgat agaagtacgc
     5881 aggcatccca ctctgaaatg atgacgcttt catctggcga ttcacgtctc cgcccagacc
     5941 aatggatgtg atgttgatga agtattgtgg atcgagccct ttctcgagag ttgtaaggcc
     6001 acagtctacc atgcgaaggc gattttgaga aagcgcctcc tggtagttgg aggaatcgcg
     6061 cagcaacgtt ttgtaaaagt ccccacccgt gcccaggttg atgacgccca gtggaacgtc
     6121 tgcgcgaagg agctttcctt cgtgaaagta gccgttgact gcttcgttga tggatccgtc
     6181 tccgcccgcg atgaggatct gatcgaaact accctgtttg agaatggagc gcacttgctc
     6241 cgtggcatct ctgggtgcct tcgtctggta aacttcgaat ggcccgagca gtttgtggag
     6301 atccggcttg agcttgttga atgccttgag ggaggctcca ttcttgcttc tcgggttgag
     6361 gatgacgact ctgcgcatgt cgtagactca taaatgagga aaaaaaagtc cagtcaattt
     6421 gttgtcccag ggtttccggc tttacacgcg acaggttgcc atcacccatt acgagtgtgc
     6481 agagcaggct acccatgcgc aatattgcag tactgagcga cgacgcggat ttgaacgcga
     6541 agatcgcctc ttcgtgcaat agctttggag acgagttcca ggcggtgttc tttcatgaag
     6601 agcgcgcatt tgttcaatat ctcaactatg agctgcctga gattgatatt atagatttct
     6661 caggttccaa agcgagcgcg ggcagagcgt ttactgagat caaagatgac ccgtggcttc
     6721 actttggtgg aagcattatc atctatgaaa aacaaaaaga agaggatttg ctcaaccgac
     6781 tgcgcggtgt gaatatcatt gcacacatcc agaagagcaa agtggagttg tatcttccgc
     6841 gagcgctgaa tgttttgtct caacacagaa gcattttgtt ccagcgcgac ttgcacgcac
     6901 tgctgcagtc caacatctcc ggtatgttcg ttctggacaa cgatccattt gatgtgacta
     6961 cttactcgaa cctgatcgcg aattttctct acaactccaa cttgatcaat ctggaccaga
     7021 agtccaattg cagcgcggcc ctggttgaac ttctctacaa cgcaatcgaa cacgggaatt
     7081 gctgcattgg atttgaagag aagaaggtct ggctggaaaa cggcggagat attctagatc
     7141 tcattcgaca aaaagcaatg aagccagaga ttctggcaaa gaagatcttt ttgaactatc
     7201 gcataacacc ggaaaggtcc tatttcaccg tccgcgacga gggggaggga tttgactggg
     7261 gatcgcgcct ggcagaggac aatccagcag aggcattgca tggtcgcggg atcatgattg
     7321 cccggaacta cctgcagaat gtgcactaca atgaaaaggg aaacgaagtc tcttttcaaa
     7381 tagaacattt acatgaatca aatgtaatcc ctcgcgtttt tgcggatcag gaggaagtga
     7441 cggttcagga tggtgaggtg atctttcgcc agggagaaac gtccagtcat ctctactata
     7501 taatttccgg gaaatacgat atcatcgcga acgggagaaa ggtttctacg ctaacaccag
     7561 cggatatctt tctcggggaa atgtcattct tactgaacaa caagagatcg gctactgtga
     7621 ttgcggtggg ccgtggtcag ctcatgaaaa tttccaaaca ggcgttcatc aacgcgattc
     7681 gcgagcatcc gcactatggt atttttcttg cgcgtcttct cgcgcaaaga ctcgaccagg
     7741 tacatgaagt tggactttga accggagttg cgcatttgat tagaacggtt ctccgcggct
     7801 ttcgccgcgt ttctgcagtg atcctgggtc ggcctgattt tgcaaatctg gaccagcggc
     7861 tcttcaacgt tatgtgcttt ggtggaacaa tcgtggcagt attgtctgcg atcgagaacg
     7921 cgttcattga cgttcccttt gtcctcgtag taggcagttg cgtgcagaca gtcattctcg
     7981 cctctggttt tgtagtttcg cgttactgga atatctacag gcccatcgta tggcccgtca
     8041 tccttgcgat tcttgtgttt ctagccatgc agtggttctg gaatgccgga tctgttggtg
     8101 gagctcatta cttcttcttc gtgtgtggca tgatgggtgc agtcatcatg agagggtggc
     8161 agaggtattt tgttatactt ctctatcttg tggtgatcaa cggagttctt tatctggaat
     8221 acattcgtcc ggatttgatt gtgaattact cggaccgaca atcgcgttac gtggacattg
     8281 ccttcagtct tagtttgtgc atgattctct tcgggcttgt gatcgcgatt ttttcagggc
     8341 attatcaggt tcttgtgaat cgggtgcaga ggcacaagct ggaatttgta gaagatctgc
     8401 atttggcgcg cattcttcag gaaactgtat tccagcatga tcgaaccctc acgcagggtt
     8461 ttgattgcga aatcgtctat cgtccgtctg ccgcgctcgg aggggattta tacgaccttt
     8521 cgcggcgtct tgggatcctt cgcgtgttta tggcggacat gaaaggtcac ggagtcaatg
     8581 ctgctttatc gtctatgctc atcaagagtg aatgggttca ttcaggccat gcttcgttta
     8641 gcgccggtga tgcgcttgcg aatttgaaca agcaggtagt ggcccgatat ggcgattctc
     8701 tggttctatc tgcaatagtt gcagatcttt acgaagacag cattcatttc gcggccgccg
     8761 gtcatgttcc tcagttcaga gtgttcggcg gagaagttct caggcttgag gcgacgggcg
     8821 ttcctatcgg agtcatggaa gattctcagt attccacgga gaggacggcg cttcctcgtg
     8881 gatccaggct tgtgttcttt acagacggtt tccatgatca accggatctt tcgggtaaga
     8941 ctattggcac ggactggatg gaagagatcc tgcgtaaatc acagccgtcg agcgcggata
     9001 tggtgagagc gctgatcagc aaatttcacg cgaagactgg aacggagccg gggaccactg
     9061 cggatgacct gactttgatt gtgatcggcc gctgagtact aatcgaatcg aatcaggata
     9121 gagcttccgt gatcttttag gaatttgcga ccttccttgt attccggtaa aacgcgttct
     9181 acttccgccc agaatcgact ggagtgattg gcgtggatga ggtgacacag ctcatgcaca
     9241 atcaggtagt cgatcacgta ttgaggcgca gccaggatct tccagtttag actgagcgat
     9301 ccagtatgtg aacagcttcc ccatctgctt ttcattccgc gtacggaaat tctggatgga
     9361 tgcttcccga cgatgggtgc aaatctggat actgtctctc ggaagatttt ttccccgtgt
     9421 ttggaataga cagccgctac tttcttcttc gcaatcagag catttcgggc atgaaccgtt
     9481 agtgattcat ccgttaggcg gctttgcctg atgccaggga gcacatgtag tagaatctct
     9541 tttccctgcc acgggatctt ctctccgtgc acataacgca gccttgctgc cgatggacgt
     9601 tcctggtttg cctgtagttt ttgacgaatc cacggagctg cctgcaaaag aaatt
//
LOCUS       DAOQJL010000084         9585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3771_length_9585_cov_3.988143, whole genome shotgun sequence.
ACCESSION   DAOQJL010000084 DAOQJL010000000
VERSION     DAOQJL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3771_length_9585_cov_3.988143"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLG78_05510"
     CDS             complement(<1..354)
                     /locus_tag="PLG78_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017855632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNE22600.1"
                     /translation="MQTLKKNDYKVKDISLARWGREEIILAEKEMPGLMALRKEYGKD
                     KPLRGAKIAGSLHMTIQTAVLIETLAELGADIRWSSCNIYSTQDHAAAAIAEAGIPVF
                     AWKGETEEEYWWCVEE"
     gene            complement(365..1312)
                     /locus_tag="PLG78_05515"
     CDS             complement(365..1312)
                     /locus_tag="PLG78_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE22601.1"
                     /translation="MGDLLKLLKSAADETRLRILSVVESEPLSVGEILEILDMGQSRI
                     SRHLKILADAGFLESNRAGNRVYYGLSAASRQEPFVHELLANLSTTAEDATYRGNGLA
                     GGLIQDRRKLTGILELRKKETREHFQKYGRDHDRLQRAYVDADFYRKEIARELGKGAG
                     VVVDLGCGTGELSALISAQARRIIGVDQSTKVLDLARKECPSGDFRLGELEHLPLKNE
                     EADAVVASMVLHHLPDPLTGLREANRVLRKNGALVVADLKQHEDEEAQKELADFWPGF
                     EKSRLETMIRDSGFEITEHRSGKGSGRLTCLIYRAKKTK"
     gene            complement(1415..3169)
                     /locus_tag="PLG78_05520"
     CDS             complement(1415..3169)
                     /locus_tag="PLG78_05520"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22602.1"
                     /translation="MSEKRTLTLAAINDITTRINSEQDLPGLLTVIMETARQLLNTEA
                     ASLLLYDPEREELIFDIVRGPRGGMLANKRIPKGRGIAGLCAEKKEPIIVDEAQTDPR
                     VMRSFDAESNFTTRNLLAVPMIARGDLIGVLEVVNTSDGRKFERHDINLMNYLANMAA
                     LAIHNRKLYDDLRNRADELNCLYEISQKIGFQNKREDLLDTIITAIGEVLVVERVSVL
                     LREGPDSTLKVARLRGFSIDDHDVRIDPEEGIAGIVFKTGDPLLVRDMEKDLRIMADK
                     SSRYKTRSFIAVPIFRDGKVAGIINAADKKNGEAFDLFELQVLSTVATQLADGLSRID
                     AKQREVQLQQYRKDLETAAQIQINSLPEIPSRIAGLQIAYRYQACRDVGGDFYDLIFH
                     SENRISVLIADVAGKGVPAALFMEYSKTLLASHIPRNLDPVTTLTRVNQEIFRKSRTG
                     IFVTSMLVQIEIDLKRLRVASAGHNNQLLLRTRRGKVEKISARGTPLGIFENTEYIEN
                     VVSFEKDDILLLYTDGVTEANNPALEEYGDERLMSIVESARGLEPHAIVEKLFQDLAV
                     FCGSAEQADDVTVVAVRL"
     gene            complement(3172..4032)
                     /gene="metF"
                     /locus_tag="PLG78_05525"
     CDS             complement(3172..4032)
                     /gene="metF"
                     /locus_tag="PLG78_05525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNE22603.1"
                     /translation="MKMITDMLKEKRPLYSFEFFPPKTSEGETKLFSTVQELSALKPD
                     FVSVTYGAGGSTRDKTRSWVETIQNRYGIPAMAHFTCVGSSRAEIRAYLEDLSRSGIR
                     NIMALRGDPPKGETSFKPAPDGLAHGSEMIAFIRQTGLPFALGGAAYPEVHPEAISPE
                     DDLVRLREKVKCGADFLVTQLFFDNSKYFSFIKKADVAVPVVPGIMPITAFQQVERFT
                     KMAGCSIPQSLLRAIADCGEDTKKLLEVSLAFTEQQCRELLDAGAPGIHFYTLNQSHA
                     TMEILERIRR"
     gene            complement(4029..6569)
                     /locus_tag="PLG78_05530"
     CDS             complement(4029..6569)
                     /locus_tag="PLG78_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNE22604.1"
                     /translation="MSSLLKISHPETLLFAQWQAPGSTDESGGRILSWQGPVMESPGL
                     LACVGTFDTIRSFNSIKLKFHSHLKEFLPNTFRFEISNDGVVWEPILHEANFHGSWMD
                     EAIWHFSLISARMIKFLFLVDRQDSSGHYLAAFGEFAVMVSGIVQVDVSSELDRLWVK
                     DNLVDQRKDYGWSSALRLKKQPEFIHLDLGSINRVGEIRLLSKNDRETFFPEAFRLSY
                     SEDNITYHHLIEETGFMAEPGTWYKWRFLPTNIRFLRIDITEGAKTREGKYVSQIIEV
                     ELYAVPDLVDKTEKSLPEPIPYASVLRSGIMRFAVDGEVKEGVAVQASDRRLRDSTTE
                     ARGIVELASDGEDSPGVAVQGSDRRLKYATEDLPGIVRLARDGETRTGHAVQGSDRRL
                     RLASEEEAGLVELAADGENRPGVVVQGSDSRLRYATEKAHGIVKLAAIGASNPGESVQ
                     ANDPRLRDATEEAKGIVRLARSGEAAGDAAVQGDDPRLRAASTENLGIVELARDGEVK
                     EGAVVQSHDKRLHPATESSPGIMEFCAPGNSIAGRAVQGHDPRLSDARDPKPHNHDYA
                     PRVHNHSDHEGSLHIRVEGGTAIKKIVSPPPSNAPVTGQSTGAGSGILGIGERDGVAG
                     AGKSAGVLGLGLTQGAGVIGAAQNGSGGWFVSERGYGLVAGGMAASHDIHSSPYALLS
                     RGLSGFLDSMVVANPELEACIAVYFPVESGDVISPGDLVSSGKPGHVKKCKTAQDTAV
                     VGVVVNRAAVVLGAPGNYLPDPAKPDGFSDPQAPKGHVLVAVSGIVSIRTVAQSGIRP
                     GDLLVASSQAGKAEKWQSDKAKPGSLVARSLEELSKGEGLLRCLLVSG"
     gene            complement(6566..7357)
                     /locus_tag="PLG78_05535"
     CDS             complement(6566..7357)
                     /locus_tag="PLG78_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE22605.1"
                     /translation="MKPDNLPFTKLVVALFDSGLWARMSAAARTLYPVLLRFSDGKFK
                     PVFPGTALLLKLTGFKQKHSIRDARNELVELGLIAITKGSGQKNTYYHFRFEWAGGPP
                     VTQGGSPGYPTEGLEAASPGGTELFSRGAGDGSPHNQIHITITNHLENRLPGELEAMI
                     KKFGEDKVRGAVTELTLAGLPANAANVEGVIQHANHAWTSLREKLTGSLSRESIRLLD
                     QAWMGETNGKLCFRDDLPGHLKKIVTRHGSGVEFVQSAVQTAATR"
     gene            complement(7650..8453)
                     /locus_tag="PLG78_05540"
     CDS             complement(7650..8453)
                     /locus_tag="PLG78_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE22606.1"
                     /translation="MSSKNKDIGLLADVFRRETLDGTITRLRLDQLKPSEYQPRQDRL
                     REIDELAASIKRDGLLHPITVTKEGDSYRIISGERRFHALNSIGAKEAECRILSREGR
                     DLQRIAIIENLQRVDLTAQEEAVALLKLKRAESYSDQDLAEKVGKSRNYVTEILGIAG
                     LPGDVLKECLNAGINNKNLMIQVVQAYKRGEYKAFLNQFKAGQIQTVKEAKEFNRPAG
                     HTALRKPASTPGPTAGGTRIQVEKNTIKIHFSSEEAAKEAAKKIRRLFG"
     gene            complement(8437..9186)
                     /locus_tag="PLG78_05545"
     CDS             complement(8437..9186)
                     /locus_tag="PLG78_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE22607.1"
                     /translation="MTAIAVANQKGGEGKTTTAINLAYGLARRKHMTLLVDLDPQANT
                     TGIFAHPDQLEKSLYDVFVGRARLSEILLQTPDPMLKVAPARISLAEFEALHNNVDAP
                     FVMRDAIQTLSGFEFIVMDSPPSLALLTINALAAATHVIIPTQPEKFSIDGMKALQAT
                     INSIKNRINPNLQILGALITQMKSKTVLTKTILPVISQFFPVFDQTVSDGVAVGESHL
                     ARKSIYEYAPDSKQAKEYTDFITEMLRELKK"
     gene            9331..>9585
                     /locus_tag="PLG78_05550"
     CDS             9331..>9585
                     /locus_tag="PLG78_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22608.1"
                     /translation="MRYLPGPYTLFAREGQAFRVVERSGYDSPFVYEFLADPDTWAGA
                     MELGQQRIEARGSRSDCEVWVAVDPIGDPVRVLVAEIPRES"
BASE COUNT         2135 a         2606 c         2332 g         2512 t
ORIGIN      
        1 ttcttctaca caccaccagt attcttcttc tgtttcccct ttccaggcaa acacaggaat
       61 tcctgcttct gcaatggcgg cagctgcatg gtcttgcgtg ctgtaaatgt tgcagctgga
      121 ccagcgaatg tccgctccaa gctcggcgag agtctcaatc aaaaccgcag tctggatcgt
      181 catgtgcaga gaacctgcaa ttttcgctcc gcgcagcggc ttatcttttc cgtattcttt
      241 gcgcagagcc atcaggcctg gcatttcctt ttcagcgagg atgatctctt ctcgtcccca
      301 gcgggccagt gaaatatcct tcactttgta atcgttcttt ttcagcgttt gcattcaaac
      361 cctcctattt ggtttttttc gctcgataaa taagacatgt aagtcgaccg gaccctttgc
      421 cggatctatg ttctgtgatt tcaaatcccg aatcgcgtat catggtttcg agcctggact
      481 tttcaaagcc cggccagaaa tcagcaagtt ctttctgcgc ctcttcgtct tcgtgctgtt
      541 tgagatccgc aaccacaagt gctccgttct ttctcagtac tcgattcgct tcgcgaagcc
      601 ccgtcaaagg atcaggaaga tgatgcaaca ccatggatgc aactaccgcg tccgcctctt
      661 cgttcttcaa aggaagatgt tccagctcgc ccagacgaaa atcgcctgac ggacattcct
      721 tgcgcgccag atccaggact tttgtgcttt gatctacgcc gataattcga cgcgcctggg
      781 ctgaaatgag cgcggaaagt tctccggtgc cgcatccaag gtccacaact actcccgcac
      841 ccttcccaag ctctcgtgcg atttctttgc ggtaaaagtc tgcgtctaca tatgcgcgct
      901 gcagacgatc atgatcgcgt ccgtactttt gaaaatgttc tcttgtttct ttcttgcgaa
      961 gctccagaat tccggtgagc tttcgcctgt cctggatgag accgcctgcc agtccattgc
     1021 ctcgatacgt tgcatcttct gccgttgtag aaaggtttgc aagaagctca tgcacgaacg
     1081 gttcctgtct ggacgcagca ctcaggccgt agtacactct gttccctgcc cgattgctct
     1141 ccagaaatcc tgcgtctgcg agaatcttaa gatggcgcga gatgcggctc tgacccatat
     1201 ccaggatttc gagaatctcc ccgacagaaa gaggctcgga ttccacgacg ctgaggatac
     1261 gcagcctggt ttcatctgcg gcggacttga gcaactttag cagatctccc atctatacca
     1321 atatcaagat atcctgatat agagtccacc cttttactgt gccgaattca aggcaatcgg
     1381 cgactgaatg ggtgaattct agctctgggc ggcctcaaag gcgaactgca acgacagtga
     1441 cgtcatctgc ctgttctgca gaaccgcaaa aaacagccag atcctgaaac agcttctcca
     1501 caattgcatg cggttcaagc ccgcgggcag actccacaat gctcatgagc ctttcatcgc
     1561 cgtattcttc cagggccggg ttgttagctt ctgtgactcc gtccgtatat aatagaagaa
     1621 tatcgtcttt ttcaaaactg accacgtttt cgatgtactc tgtattctcg aagatcccga
     1681 gaggtgttcc tctcgcagat atcttctcca ccttcccccg gcgggtgcga agaagaagct
     1741 ggttgttatg gccggcggag gcaactctca ggcgtttcag atcgatttcg atttgaacca
     1801 gcatggatgt aacaaaaatg ccagtgcggg acttgcgaaa gatctcctga ttcacccggg
     1861 tcagagtggt cacaggatcc agatttcgcg ggatatggga cgcaaggaga gttttggagt
     1921 actccataaa aagagccgct ggaactccct tccctgctac gtccgcaatc agtactgaaa
     1981 ttctattttc agaatgaaag atcaaatcgt aaaagtcgcc gcccacgtcg cggcaggcct
     2041 ggtaacgata ggcgatctgc agacctgcaa tgcgcgaagg aatctccggc aaagaattga
     2101 tctggatctg cgctgccgtt tccaggtctt ttctgtattg ctgaagctgc acttctcttt
     2161 gctttgcgtc aatgcgagac agtccgtctg caagctgtgt tgcgactgtg gaaagaacct
     2221 ggagttcaaa tagatcgaag gcttctccat ttttcttgtc tgcggcattg atgataccag
     2281 ccaccttccc gtcgcgaaag atggggactg caataaaaga tctggtcttg tacctggacg
     2341 acttgtctgc cataatacgc agatcttttt ccatgtcgcg aaccagaagc ggatctccgg
     2401 tcttaaaaac aattcccgca attccttcct ctggatcaat gcgaacgtca tgatcatcaa
     2461 tagaaaaacc gcgaaggcgt gccactttca gagtgctatc cggcccctcc cgcaatagca
     2521 cactcaccct ttctactacg agcacttccc cgattgcagt gatgatcgta tcgagcaaat
     2581 cttctctctt attctgaaat cctatcttct gcgaaatctc ataaagacag ttcaactcgt
     2641 ccgcgcggtt tcgcagatca tcgtacaact ttcgattgtg aattgcaagg gccgccatgt
     2701 tggccagata gttcatcaaa ttgatatcgt ggcgctcaaa ctttcttccg tctgacgtgt
     2761 tcacaacttc gagaacgcca atgagatctc cgcgtgcgat cataggaacc gcgagaagat
     2821 tgcgcgtggt gaagttagat tctgcatcaa agctgcgcat tactctcgga tccgtttgcg
     2881 cctcatctac aatgattggt tcttttttct cagcacagag gcccgcgatt ccccttccct
     2941 ttggaattct cttattggca agcatgcctc cacggggacc tcttacaatg tcgaagatca
     3001 actcttcgcg ctcaggatcg tagagcaaaa gcgatgcggc ttccgtattc aaaagctgcc
     3061 gagcggtttc cataatcacc gtaagcaacc cgggcagatc ttgctccgag ttgatccgcg
     3121 tcgtgatatc gttgatcgcg gccagtgtaa gggttctttt ctcggacata gttaacgccg
     3181 gattctctcc aggatctcca tggttgcatg agattggttg agagtataga agtgaatccc
     3241 aggagcgccc gcatccagaa gttctctgca ttgctgctct gtgaaagcca ggcttacctc
     3301 aagcaatttc tttgtgtctt ccccgcaatc agcaatcgca cggagaagag actgcggaat
     3361 actacaacct gccatcttcg taaatcgctc cacctgctgg aacgcagtta tgggcatgat
     3421 ccccggaacc acgggaaccg ccacatctgc tttcttaata aaactaaagt atttagaatt
     3481 gtcaaagaat agctgtgtca ctaaaaaatc ggcaccgcac ttgacttttt cgcgcaagcg
     3541 aaccaggtcg tcttccggag atattgcttc tgggtgcacc tccgggtatg cggctccacc
     3601 gagcgcaaaa ggcaaaccgg tctgacgaat aaaagcaatc atctcgcttc cgtgagcaag
     3661 tccatccgga gccggcttga aggaagtctc ccctttcgga ggatcgcctc tgagcgccat
     3721 gatgtttcta atcccgctgc ggctcaggtc ctccaggtaa gcccggatct cagcacgcga
     3781 agagcccacg catgtaaaat gagccatcgc cgggattcca tagcgatttt gaatcgtttc
     3841 tacccagctg cgcgttttgt ctcgagtgga accacccgca ccataggtaa cagaaacaaa
     3901 atcaggcttt aaagcagaca gttcctggac agttgagaaa agctttgttt ccccttccga
     3961 agttttaggc gggaagaatt caaaagagta aagcggccgc ttctccttga gcatgtctgt
     4021 aatcatcttc atccggatac gagcaggcag cgcagaagcc cttccccttt tgaaagttct
     4081 tccaggctcc gtgccacgag gctacctggt tttgctttgt cgctttgcca tttttcagct
     4141 ttgcccgctt gcgaagatgc aacgagcaga tcgccaggcc gaattccgga ttgtgcaacg
     4201 gttcgaatcg atacaatacc agaaacggca acaagcacgt ggcctttggg tgcctgggga
     4261 tcgctgaaac catccggctt cgctggatca gggagataat ttccgggagc acccagcacg
     4321 actgctgcgc gattcacgac tacaccaacc acagccgtat cctgagcagt cttgcatttc
     4381 ttcacgtgac caggctttcc agaggaaaca agatctcctg gactgattac atcgcccgat
     4441 tcaaccggga aatacactgc aatgcatgct tccagttcgg gattggccac caccatcgag
     4501 tcgagaaagc cagacagacc ccgagacaat agtgcataag gagaggaatg aatatcatgc
     4561 gaagctgcca ttcctcctgc cacaagtcca tagcctctct cggatacaaa ccagccacct
     4621 gacccgttct gcgctgcgcc aatcacaccg gcgccctgag ttaaaccaag cccgagcaca
     4681 cctgccgatt ttccggcgcc cgccactccg tctctttctc cgattccgag tatcccagaa
     4741 cctgctcccg tactctgccc tgttacaggt gcattcgaag gaggcggaga aacgatcttc
     4801 ttgatcgcag tccccccttc aacgcgaata tgcaaacttc cttcatgatc tgagtgattg
     4861 tgaactcgcg gcgcataatc gtggttatgc ggctttggat cccgtgcatc tgaaagacga
     4921 ggatcgtgtc cttgcactgc gcgcccggcg attgaattgc ccggggcaca aaattccata
     4981 attccagggg atgattctgt agccggatga agtcgtttgt catgactctg aacgacggcc
     5041 ccttccttca cttctccatc acgggccagc tccacaatcc cgagattctc tgtggacgct
     5101 gcacgaaggc gcggatcatc cccttgaacg gcggcgtctc cggcggcctc accgcttcgt
     5161 gccagacgaa cgatcccctt cgcttcttcc gttgcatcgc gcaacctggg atcattcgcc
     5221 tgaacgcttt cgccaggatt gctcgcacca atggctgcaa gcttgacaat gccgtgagcc
     5281 ttctctgttg catatctgag gcgactatcg ctgccttgca ccacaactcc aggtctgttt
     5341 tccccatcgg ctgcgagctc tacaagacct gcttcttctt cactcgccag gcgaagcctg
     5401 cgatcgcttc cctgaaccgc gtgacccgta cgtgtttctc cgtcgcgcgc cagtctcaca
     5461 attccaggaa gatcttccgt tgcatatttc aaccttcgat cgctaccctg aacagccact
     5521 cccggagagt cttccccgtc tgaggcaagc tctacaatgc cgcgggcctc tgtggtggaa
     5581 tcgcgaagtc gtctatcgct tgcctgaaca gccacccctt cctttacttc tccgtccacg
     5641 gcgaagcgca taattccgct tcgtaaaaca gaagcataag ggatgggttc aggaagactc
     5701 ttttctgtct tgtctacaag atccggaaca gcatacaatt ccacttcgat gatttgagat
     5761 acgtacttcc cttcgcgcgt ctttgcacct tccgtaatgt caatgcgcaa gaaacgaatg
     5821 ttcgtcggaa ggaatctcca tttataccag gtgccgggct ccgccatgaa tccggtttct
     5881 tctataagat gatgatacgt aatattgtct tcgctgtaag atagcctgaa cgcttcagga
     5941 aaaaatgttt cgcgatcgtt ctttgataag agtcgtattt ctccaacacg attgatggat
     6001 ccaagatcca gatgaataaa ttcaggttgc ttcttcaggc gaagagcgga agaccagccg
     6061 tagtcctttc tctggtctac gagattgtct ttcacccaca gtcgatccag ttcactggac
     6121 acatccacct ggacaattcc cgaaaccatg accgcaaatt ctccaaaggc agccagatag
     6181 tgtccggatg aatcctgcct gtccacgagg aacagaaact taatcatgcg agcggatatg
     6241 agggaaaagt gccagattgc ttcatccatc cagctgccat gaaaattcgc ttcgtgcagg
     6301 atgggttccc atacgactcc atcattggag atttcaaatc taaatgtgtt tggaagaaat
     6361 tcctttagat gagaatggaa cttcagcttg attgaattaa aagaacgaat tgtatcaaaa
     6421 gtcccgacgc aggcaagcag tcccgggctc tccatgacgg ggccttgcca ggacaggatg
     6481 cgaccacccg actcatcggt actgccgggt gcttgccatt gggcaaaaag taatgtttcg
     6541 ggatgtgaaa tctttaggag tgaactcatc tggtggctgc cgtctgaact gcagattgaa
     6601 caaattctac gccacttcca tggcgagtta caattttttt cagatgcccg gggaggtcat
     6661 cacgaaagca cagctttcca ttcgtttctc ccatccacgc ctggtctagc aagcgaatgc
     6721 tttctcgaga cagggagccc gtaagttttt ctctgagaga tgtccatgcg tgatttgcgt
     6781 gctggatgac accttctacg ttcgccgcgt tggcaggcaa accggccaga gttagctcag
     6841 tgacggctcc acgcactttg tcttcaccga attttttaat cattgcttcg agttccccgg
     6901 gaagcctatt ctctagatga ttggttatgg ttatatgaat ctggttatgc ggggaaccgt
     6961 cccctgcccc tctcgagaac agttcggtac cccccggaga ggcggcctcc agcccctcgg
     7021 tggggtagcc gggagatccc ccctgggtga ccgggggacc ccccgcccat tcaaatctga
     7081 aatgatagta cgtgttcttc tggccggatc cctttgtgat ggcgatgagc cctagctcaa
     7141 cgagttcgtt cctggcgtcg cggatgctgt gcttctgctt aaacccggtc agttttaaca
     7201 gcaaggctgt cccaggaaaa acgggtttga acttgccgtc cgaaaaacgg agtaagacgg
     7261 gatagagcgt tctggctgcc gcagacatcc tggcccacag gccggagtca aacagagcca
     7321 ccaccagctt ggtgaaaggc agattgtctg gtttcatgcg gattctctaa tttcagtgta
     7381 gagaagccaa atttgttgac aaatcgtggt acagcaggta tttatgtgac ataatcttga
     7441 aaccccggcc ctccgttcca gcggagggct tttttttacg ctttcgcgtg tctcattcct
     7501 gacgaaaaga gacatccaaa atattatgtc acattacggg tacgtcaaat cattttctta
     7561 aaaaaatcca ggcaattagt atgtattaca tactaattgg attcttgcgc ttgtcgggtt
     7621 gtgttgtttt taaaactgct ttttcctggt cacccaaaaa gtcgccggat cttcttcgct
     7681 gcttccttcg ccgcctcttc cgatgagaag tgaatcttga tcgtattctt ttccacctgg
     7741 atccgggtgc cgccggcagt tgggccgggt gtcgacgctg gctttcttaa tgctgtgtgt
     7801 ccagccggtc gattgaattc ttttgcttct ttgacagtct gaatctgccc ggctttgaac
     7861 tgattcagga atgctttgta ctctcccctc ttgtaagctt gcaccacctg gatcatcaag
     7921 ttcttgttat tgatgccggc attcaagcat tccttgagca catccccagg caatcccgcg
     7981 atccccagaa tctcagttac ataatttctg gacttcccta ctttttcagc cagatcctga
     8041 tcgctgtagc tctctgctct cttcaacttt agaagtgcaa ccgcttcttc ttgagcagtc
     8101 aggtccactc tctgcaggtt ctcaatgatg gcgattctct gcaagtctcg cccttcgcgt
     8161 gatagaatgc ggcactctgc ttcttttgcg cctatgctat tcagtgcgtg gaatcgtctt
     8221 tcccccgaga tgattctgta tgagtctccc tcctttgtga ccgtgatagg gtggaggagg
     8281 ccgtctctct tgatgctggc cgccagttcg tcgatctccc gcagacgatc ctgtctgggt
     8341 tgatattcag aaggcttcag ctgatccagt ctgagtcttg tgatggtgcc gtcgagagtc
     8401 tccctgcgga agacgtcggc gagtagtcca atgtctttat tttttgagct cacggagcat
     8461 ctccgttatg aagtctgtgt attctttggc ctgctttgag tccggtgcat attcataaat
     8521 acttttgcgc gccaggtgac tttccccaac cgctactccg tcactgaccg tctggtcaaa
     8581 gaccgggaaa aattgcgaaa tgaccggcaa tatcgttttg gtgaggactg tctttgattt
     8641 catttgagtg atgagcgccc ccaggatctg gaggttcggg ttgatcctgt ttttgatgga
     8701 gttgatcgtg gcctgcagag ccttcatccc gtcgatcgag aacttttccg gttgggtcgg
     8761 aatgatgaca tgggtggccg ctgccagagc gttgatcgtg aggagggcca ggctgggcgg
     8821 gctatccatt acaatgaact caaatccgct gagagtctga atcgcgtcgc gcattacgaa
     8881 gggcgcgtct acgttgttgt gcaaagcttc gaattcagcg aggctgattc gggcgggcgc
     8941 taccttgagc atcgggtccg gggtctgcaa gagaatttcg gaaagcctgg ctcttcctac
     9001 gaacacatcg tacaaagact tttcaagctg atcgggatgc gcaaatatgc cagttgtatt
     9061 ggcctggggg tccaggtcca cgaggagggt catgtgtttg cgtctagcca gtccgtacgc
     9121 gagattgatg gcggtagttg tttttccctc ccctcccttc tggttggcca cggcaatggc
     9181 tgtaatcttc atgctctgat gtgacataat aaaggaagca atgatgtcaa ccagaaaaag
     9241 gaatcatcct gaaaaatatg tcggatgtcc gacttgcggg gccgggattc tgtcggatgt
     9301 ccgacatagg ggcgcttctt gaccgacctg gtgcgctatc ttcccggccc atataccctg
     9361 tttgctagag aagggcaggc ctttcgcgtg gtcgaaaggt cggggtatga ttctcctttc
     9421 gtgtacgaat tcctggccga tccggatacc tgggccggcg ccatggagct tggccagcaa
     9481 aggatcgagg cgagggggtc caggtcagat tgtgaagtct gggttgctgt tgatcccatc
     9541 ggcgatccgg ttcgtgttct ggtcgcggaa attcctcgtg agagt
//
LOCUS       DAOQJL010000085         9571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3786_length_9571_cov_3.917297, whole genome shotgun sequence.
ACCESSION   DAOQJL010000085 DAOQJL010000000
VERSION     DAOQJL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3786_length_9571_cov_3.917297"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLG78_05555"
     CDS             <1..290
                     /locus_tag="PLG78_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22609.1"
                     /translation="PTGGNSLASRVAVAGLRAKLGKPFPQVLWDGIVNKARFPDRKLT
                     PDAQICIRDNGNADFGNFDAANNMSDIKRDLAVHNCSHPALSPNRIPGVRD"
     gene            287..1378
                     /locus_tag="PLG78_05560"
     CDS             287..1378
                     /locus_tag="PLG78_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HNE22610.1"
                     /translation="MKAIALVLLVAAFSATGCKKSDGVQFYDDNFPAKLSDWKLFVRS
                     GENLTMAAGLIPYDLNSPLFSDYARKFRTVYVPPGKSAEYTKIGVLNLPVGTILSKTF
                     YYPRKAGAVESSDFPSMKENHVIETRLLVHTSDGWVGLPYIWNKEGTEATLEITGGDS
                     NVQSFDMDGKPIAFKYSVPNQGQCSGCHSARRGEDKVMTPIGPKARQLNKVYAYKGGP
                     ENQIEHWKKSGILNVPADAVVVRNAVWNDPQTGTVEERARAYLDVNCAHCHIEYGPAN
                     QSGLILSLENQEPHRFGVCKSPTAAGRASANMDYDIVPGNPDSSILIHRMTSTEAGIR
                     MPELARSLLHKEGLSLVREWVKQMKGNCN"
     gene            complement(1384..1842)
                     /locus_tag="PLG78_05565"
     CDS             complement(1384..1842)
                     /locus_tag="PLG78_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE22611.1"
                     /translation="MAGKRTADEQHIAELERTLEAIRRKDRLMTESQSTVSRILLNAP
                     VMIYVYDLVDKRNVYINREATDFLGYSSDQIRDLGPDLLSTLIHPEDLPRAIAHHNRL
                     LGATGPLKLVYRMKNAKGEWRNFLSCDTPFETEPGEQTRFILGCVQEMPD"
     gene            1940..3451
                     /locus_tag="PLG78_05570"
     CDS             1940..3451
                     /locus_tag="PLG78_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22612.1"
                     /translation="MLNPFRPAEHQPLLPVEEQNRRIAPLRWQILEATFLGYTFYYFT
                     RNNLPIVSLEAASDLTYSSAQMGLLMGATGLAYGIGKFILGALSDRSNPRAFMPAGLL
                     LSAALNFLFGAASNFWIHLGLWTLNGFVQGAGWPPCGRSLGHWYSKSERGTVFGFWNI
                     AHNIGGGLIGPLAAAAASAFGWRSAFYIPGILAVLAAVYLMIRLRDTPQSVGLRSIEA
                     FPPMSNAKYLIALIASVLTGNAIAGAVMYTLLPYEQLPAAVTMLNLVLIPACFWVIGM
                     LPGNRASPPSRLSAAIPAHDVSLEKELSLWEILWNNVLTNKIIWLFAIANLFVYVVRY
                     SLIDWGPTYLKQAKGASMQTGGFSTLAFEASAIVSTLLIGWISDKSGGRRGMVSLICM
                     IPVFLAFLGMIVMPPGFLWVHIVLFGVVGFFIYPPVMLLGVAGLDFTSKKAVGAAAGF
                     IGLLGYTGKFVEGIALGVLSKELGWPGALSFIAASCIVAILMLAFTWKLKPAE"
     gene            3487..4107
                     /locus_tag="PLG78_05575"
     CDS             3487..4107
                     /locus_tag="PLG78_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22613.1"
                     /translation="MIKPEKAAWELKNEKNKAGVRWLLVVLIGGYLGSLLQNGGVLPG
                     ADRLFSWYYIGAVTAFVAILNLAVTLYILRAKDQGTFHPAVKYLTMVTDLLAVSLVLL
                     PTGGSESMFFLVYFVIIVSNALRYGMRLAVLGVLVFNILYVLVLAYQYAPNFDIPGFQ
                     KEVLKVSGVWLIGLYTGYLARRFEMLQGEVEKYQKLLANLMAEKKQ"
     gene            4104..4484
                     /locus_tag="PLG78_05580"
     CDS             4104..4484
                     /locus_tag="PLG78_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22614.1"
                     /translation="MTEKNPQHENAELEASVQKAFRRGNINFDFLQPDSTPREIAEEE
                     RSERRTNSRQGKRGLFNRGRPSRPISRAQVYVWAALFGVSVTLVLVLFFSGKSILLLL
                     GLPILFGTTLWSVIMLTLFLARPR"
     gene            complement(4450..5196)
                     /locus_tag="PLG78_05585"
     CDS             complement(4450..5196)
                     /locus_tag="PLG78_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22615.1"
                     /translation="MSLPSHPVRRALYRLVTSLFASVSTILLSVYQCTLEFEVEGEDH
                     LRLLKAKGENYVVAVWHTFVDAAVFCLHHRNLVIYSDHPRTPEYENSWTHFFREIGLK
                     TIRALGFEVLDASLGKQSAGIINFIKKIQSGSPALVAPDGPHGPIYESKPGVLYMAHK
                     ASSVVVPVGVGFSRRIVGPNWDDFNLPLPFSRVALVIGEPIYPSDASEEALKTKAREL
                     EETMDRLCFRANEILFGPASSEAAQEKVSA"
     gene            complement(5212..6954)
                     /locus_tag="PLG78_05590"
     CDS             complement(5212..6954)
                     /locus_tag="PLG78_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNE22616.1"
                     /translation="MAAAQPVGDPQTGAPVPAQPSQAPVPYEQTNQPAARPSSTPASA
                     TTTPAPQTQAAQTPAAEEALPESWLDGFKWKALVRLRPESRGNFDFDKKTDDNAEYVG
                     QKIQFGFEKDFFDDMSANVVVQDSRVWGGQPGSKTGVSTANDSTSESTDLRIGWLEMR
                     SLGPFDVRFGRQVFRLGEERLVGAAEWGNVGRSFDGFSVRTTNSFYTGQLIGSVLSEE
                     DNDLSSNGTKVGPSNSSGFTFTCDSTTTVCTVKARTVREQGDSYLAGFYNTFTAGELF
                     QFEAYYLGVYKKWIQKTSPLALPDAELMSQDRSRQRDNLHTIGARLTNRMGTDRKAQW
                     EIPIDWSIEYAWQSGPTGRRIDASWDYLRVTVPSPAGPVRAYTEREKYNAYAFAADAG
                     YTIASFLRIGVAYNIASGDKNRSDGVVTTFNSLFPTSHGLYGQADLVSWQNMIGKSIQ
                     MNLMFGKFGKLKLAAWQIDKMRLEDGWYDGTGTLKTNESTESFSNTRFSPLQDSSGYR
                     ASAYLRKGLLKEYDLTYIVPWKRVEIGLGYSRIQAGDSIREAKNDTLNTTISRRPRFD
                     PRADFAYLMLTMSF"
     gene            complement(7001..7819)
                     /locus_tag="PLG78_05595"
     CDS             complement(7001..7819)
                     /locus_tag="PLG78_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE22617.1"
                     /translation="MIPSNELFVPWDALPHRFRLQDTALSSGYVQRCKLKNPKTKRMR
                     FVSSLLLLAAASVTLIPLGADNDRALVRSLIGSATVLRDGKTEALRVGSVVKDSDRIK
                     TGRQSQVIVFYKGVEIRLLGETDATLVSLTNMSQPGEVSVTKGFGWFRVDDPTKRGFK
                     ASTPTSIAAVRGTKFAVSYDEKGSTSCVCQGTVATSAVADPTKENLVTAGGSHAYASS
                     GNMESHDFKKYFKKLKVDRSFQQEILRDAKLNSCKSCHRMTNIATDNSQDPQNY"
     gene            8517..>9571
                     /locus_tag="PLG78_05600"
     CDS             8517..>9571
                     /locus_tag="PLG78_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE22618.1"
                     /translation="MYKKLEIYKSDMKYKARTLSLLLFAVSWSPAMAADPVLLEAGRG
                     VYRLGPNLEILTDLEGKLTIDQITSGNYTSSFYGYGWDRVPNFGYSSATFWVRCRITN
                     PLPFQKEVLLELANPLLDEARLFFVDEGASSRTITESKSIKHRVYIFPVHVRPHETVD
                     LYLRVKSKGSIALPLTLFRPDAFAREDQDTQFALGIYYGLIAVMILYNLTLFIALRER
                     AYLYYSLALFCIHGMLQFAVNGLARKYLPLSASWWSLGSIPFFASLGIFWMLLFCRHF
                     LNTRLRTPEIHRVTTALLVVSLLMTIGSLFMPYRAAVTILTIHGLAGLFCVIAAGAYS
                     WRESFAPARYYLLGWSVV"
BASE COUNT         2276 a         2517 c         2382 g         2396 t
ORIGIN      
        1 atcctaccgg tggaaacagc cttgcatcca gagttgcagt cgcaggtctt cgtgcgaagc
       61 taggcaaacc attccctcag gttctctggg atggaattgt aaataaagcc aggtttccag
      121 atcgcaaact tacaccggat gcacagatct gcattcgcga caacggcaat gccgatttcg
      181 gaaattttga tgcggcaaac aacatgagcg atatcaaacg agatcttgca gtccacaact
      241 gctctcatcc tgcgctttcc cccaatcgca ttccgggagt gcgtgattga aagcgatcgc
      301 tctggtgctg ctggtggcgg cgttttccgc gactggatgc aagaaatcag atggtgttca
      361 gttctacgat gacaacttcc ccgcgaagtt gtcagactgg aaactcttcg ttcgatccgg
      421 agagaatctg actatggcag cgggcctcat tccgtacgat ctaaacagcc cacttttttc
      481 agactatgcg cgcaaatttc gcacagtcta cgtgccgcct ggaaagtcag ccgaatacac
      541 aaagatcggt gttctgaatt tgcccgtagg aaccattctc tccaaaacat tctattaccc
      601 cagaaaggca ggtgcagtgg aatcgtctga ctttccgtcg atgaaagaga atcatgtaat
      661 tgaaaccaga ttgctcgtgc acacatcaga cggatgggtc ggtctgccct atatctggaa
      721 caaggaagga acggaagcaa cgctcgagat cacaggcgga gactcaaacg ttcagtcttt
      781 cgatatggac ggaaaaccaa tcgcatttaa gtattcagtt ccaaaccagg gacagtgctc
      841 gggctgtcat tcagccagac gcggcgaaga caaagtcatg acgccgattg gaccaaaagc
      901 gcgtcagctc aacaaagtct acgcgtacaa aggcggtcca gaaaaccaga tcgagcattg
      961 gaagaagtct ggaattctca atgtccccgc ggatgcagtt gttgtgcgaa atgcagtatg
     1021 gaatgatccg caaacaggaa cagtcgaaga aagagcacgc gcgtatctcg acgttaactg
     1081 cgcgcattgc cacatagaat atgggccagc gaatcaatcc ggtttgattt tgagtttgga
     1141 aaatcaggaa ccacacagat tcggtgtttg caaatcgcca actgcagccg gtcgcgcgtc
     1201 tgcgaatatg gactatgaca ttgtgcctgg aaatccggat tcttctattc tgattcatcg
     1261 catgacttca acggaggctg gaatcagaat gcccgaactg gcaagaagcc ttctccacaa
     1321 agagggattg agcctggtcc gcgaatgggt gaaacagatg aaaggaaact gcaactagag
     1381 aagttagtct ggcatttcct gaacgcatcc aagaatgaac cgggtctgtt ctccgggttc
     1441 tgtttcgaaa ggagtatcgc aggataggaa attcctccat tctccttttg cgttcttcat
     1501 gcgatacacg agtttcaaag ggcccgtggc accgagcaaa cgattgtgat gcgcaatcgc
     1561 gcgaggaaga tcttccggat gaatcagagt ggaaagaagg tccggcccaa ggtcgcgaat
     1621 ttgatccgat gaatatccta gaaagtccgt ggcttcgcgg ttgatgtaaa cgtttctctt
     1681 atccactaga tcgtaaacgt aaatcatcac aggcgcattc aatagaattc tgctgaccgt
     1741 agactggctc tcggtcatca aacgatcctt gcgcctgatc gcttcgagag ttctttcgag
     1801 ctcagcgatg tgctgctcgt ccgctgttcg tttcccggcc atctctatca atggaacatt
     1861 cagcggacaa agtgcaatct tttttctctg aagcccgggc gttggttgac atgctctaaa
     1921 tcgttccatc gtatgtcgca tgctgaatcc atttcgtccg gccgagcacc agcctcttct
     1981 gccggtggaa gagcagaatc gcagaattgc ccctcttcgc tggcagattc tagaagcgac
     2041 tttcctgggt tatactttct attactttac cagaaacaac ctgcccatcg tctctcttga
     2101 agccgcttcc gatctcactt acagttcggc gcaaatggga ttgctcatgg gtgcgaccgg
     2161 tctcgcatac ggaattggaa aattcatcct gggcgccctc tccgacagat ccaatccaag
     2221 ggcattcatg ccggcaggac ttcttttgtc agctgcactg aattttttgt ttggagccgc
     2281 gtctaacttc tggattcacc tcggactctg gactctcaat ggatttgttc agggggcagg
     2341 ctggcctccg tgcggacggt ccctcggaca ctggtattct aaatcggaac gcggaaccgt
     2401 ttttggattc tggaacatag cgcacaacat cggaggcggt ttgattggac cacttgctgc
     2461 cgctgccgca tctgcgtttg gatggagatc tgcgttttat atcccgggga tcctcgccgt
     2521 tcttgccgca gtgtatctga tgattcgact gcgagacact ccgcaaagcg tgggcctgag
     2581 atccatcgaa gcgtttcctc ctatgtcgaa tgcgaagtac ctcatcgcac tgattgcatc
     2641 tgttctgaca ggaaatgcaa tcgcaggcgc ggtcatgtat actctgcttc cctacgaaca
     2701 gcttccggca gcggttacta tgttgaacct ggttctcata cctgcttgct tctgggtgat
     2761 cggaatgctg ccaggcaatc gcgccagtcc accttcgcgc ttgtctgcgg cgattccagc
     2821 gcatgacgtc tctcttgaga aggaactgtc cttgtgggaa atattgtgga acaacgtact
     2881 gacgaacaaa atcatctggc tattcgcgat tgcaaatctg tttgtatacg ttgtgagata
     2941 cagcctcata gactggggac ctacctatct caagcaggcg aaaggcgcga gcatgcagac
     3001 aggtggattt agcactcttg catttgaagc aagcgccatc gtcagcactc tgttgattgg
     3061 atggatttca gacaagtcag gcggaagacg aggcatggtg agtttgatat gcatgattcc
     3121 ggtgttcctg gccttcctcg ggatgattgt gatgcctcct ggatttctct gggtgcacat
     3181 tgttttgttt ggagtagttg gatttttcat ctatccgccg gtcatgcttc tgggagtggc
     3241 aggtctggat tttacgtcga agaaagctgt gggggccgct gcaggattca tcggactcct
     3301 gggctatacc ggaaaattcg tagaaggaat cgcactgggc gttttatcca aagaactggg
     3361 atggccaggc gcgctcagtt tcatcgcagc gtcctgcatc gttgcgattc tgatgcttgc
     3421 gtttacctgg aaattgaaac cggcagaata gttgacacga tctgcggcga agagtgactc
     3481 aaacagatga tcaagccaga aaaagcagcc tgggaactga aaaacgaaaa gaataaggca
     3541 ggtgtgcgct ggctgctggt agttctcatt ggcggctatc taggttctct tttgcaaaat
     3601 ggaggagtgc ttccaggtgc tgacagactc ttttcctggt attacattgg ggcggtcact
     3661 gctttcgtcg ccattctcaa tctggctgtg actctctaca ttcttcgcgc gaaggatcag
     3721 ggcacatttc atcctgcagt gaagtatctt accatggtca cggatctctt ggcagtctct
     3781 ctggtccttc ttcccacagg cggaagcgaa agcatgttct ttcttgtata tttcgtaatc
     3841 atagtttcaa acgcactgcg atacgggatg aggctcgccg tcctgggagt gctggtgttc
     3901 aatattttgt atgttctggt gctcgcttat caatacgctc ccaatttcga cattccagga
     3961 tttcaaaaag aagttctgaa ggtttcaggt gtatggctca ttggactcta cacgggctac
     4021 ctggcgcgaa gattcgaaat gctgcaggga gaagtggaaa aataccagaa gctcctggcc
     4081 aacctgatgg ctgagaaaaa acaatgaccg aaaaaaatcc acagcacgaa aacgcggaac
     4141 tggaagccag cgttcagaag gcattccgac gcggaaacat caactttgat ttcttacaac
     4201 cggactctac tccacgcgaa atcgcagaag aagaaagatc agaaagacgc accaactctc
     4261 gccagggaaa acgcggcctc ttcaaccgcg gccgtccatc cagacccatt tcgcgcgcac
     4321 aggtttatgt atgggctgct ctttttggag tcagcgtcac tctggttctg gttcttttct
     4381 tttccggcaa aagcattctg ctgttgcttg gacttcccat tctattcggg actacactgt
     4441 ggtccgtaat tatgctgaca ctttttcttg cgcggcctcg ctagaggccg gtccaaacaa
     4501 gatttcattt gcgcgaaagc aaagtctatc catggtttct tctagttcgc gcgcttttgt
     4561 tttgagcgct tcctccgaag catccgatgg gtagatgggt tcaccaatca caagtgcgac
     4621 gcgcgaaaac ggaagaggca aattgaagtc atcccagtta ggaccaacaa tccgcctgga
     4681 aaaaccaacg cctaccggaa ccacaacgga actggctttg tgtgccatgt agagcacacc
     4741 gggcttggat tcgtagatgg gcccgtgagg accgtccgga gctacgagag cgggagatcc
     4801 gctctgtatt ttcttaataa agtttatgat tcccgctgat tgctttccca gagaagcgtc
     4861 cagcacctcg aatccaagag cgcggatggt ctttaaacca atctcgcgaa aaaaatgagt
     4921 ccagctattc tcatactcgg gagtgcgagg atgatctgaa tagatcacga ggtttcgatg
     4981 gtggaggcag aagaccgccg catccacgaa tgtgtgccag actgccacca cgtagttttc
     5041 tcccttcgcc ttcaaaaggc gaaggtgatc ttctccctct acttcaaact cgagtgtgca
     5101 ctgatagaca ctgagaagga tcgtgctcac agaagcaaac aggctggtga ccaggcgata
     5161 gagagctcga cgcactggat gagatggcag agacatgctt cctccggact gctagaaact
     5221 catggtaagc atcagatacg cgaaatctgc gcgcggatca aatctaggcc tgcgcgaaat
     5281 ggtagtgttc agcgtatcgt tctttgcttc gcgaatcgag tctccggcct gaattcggct
     5341 gtagcccaat ccaatttcaa ctcttttcca gggaacaata tacgtaaggt cgtattcttt
     5401 tagcaaaccc tttcgcagat acgcggatgc acgatatccc gatgagtctt gcagaggcga
     5461 aaagcgcgtg ttcgaaaagg attccgtgga ctcgtttgtt ttcagtgtgc ctgtgccatc
     5521 ataccagccg tcttcgagac gcatcttgtc tatttgccag gccgcgagtt tcaactttcc
     5581 aaacttacca aacatgagat tcatctgaat cgatttgcca atcatattct gccagcttac
     5641 cagatctgcc tggccataga gaccatgact cgttggaaaa aggctgttga aagtagtgac
     5701 cactccatcg gatcggttct tatcaccgct ggcgatgtta taagcaactc cgattcgcag
     5761 aaaggacgcg atcgtgtatc cagcatccgc ggcgaatgca tatgcattgt atttctctcg
     5821 ttcagtgtag gcgcgcacgg ggcctgcagg ggaagggact gtgactctca agtaatccca
     5881 gcttgcatct atgcgccttc cggtgggccc ggattgccag gcatattcta tggaccagtc
     5941 gattggaatt tcccattggg cctttctatc ggtgcccatg cggttggtga gtcttgcacc
     6001 aatcgtgtga aggttgtctc gctgcctgct tctatcctgg gacatcaact cagcgtccgg
     6061 tagtgccaga gggctggttt tttgaatcca cttcttgtag actcccagat aatacgcctc
     6121 gaactgaaaa agttccccgg ccgtaaatgt attgtagaag ccggcaagat acgaatcccc
     6181 ctgctctctg accgtgcgcg ctttgactgt gcacacggtc gtagttgaat cgcaagtaaa
     6241 tgtaaagccg gatgaattcg aaggaccaac ctttgtgccg ttagacgaca aatcgttgtc
     6301 ttcttctgaa agaacagatc cgatcagctg accggtgtag aaactgtttg tcgttcgcac
     6361 agaaaagcca tcgaagcttc gtccaacatt tccccattca gcggccccca caagcctctc
     6421 ctcgcccagg cgaaagacct gcctgccaaa acgcacatca aatgggccaa ggctgcgcat
     6481 ttccagccat ccaatgcgaa ggtctgtcga ttcggaagta gaatcgtttg cggtggaaac
     6541 tccagtcttt gaaccaggct ggccacccca gaccctggaa tcctgcacca caacgttagc
     6601 cgacatatcg tcgaagaaat ccttttcaaa tccaaactga atcttctggc caacgtactc
     6661 tgcgttatcg tccgtctttt tatcaaaatc aaaatttccg cgcgattccg gcctgagtct
     6721 gacgagagcc ttccacttga atccatcgag ccaggattca ggcagagcct cttctgcggc
     6781 cggagtctgc gcggcctgtg tttgtggggc aggagtcgtt gttgccgatg cgggtgtgga
     6841 cgaaggtcgg gcggccggct ggtttgtctg ctcataaggt acaggagcct gcgatggctg
     6901 tgctggtact ggcgcgccgg tctgaggatc gccgacaggc tgggctgcag ccatgccggc
     6961 aaaaattaga ataacaacag ctacaccaac cctcatattc tcaataattt tgaggatctt
     7021 gagaattgtc agtcgctata ttagtcattc tatgacagct cttgcagctg ttgagctttg
     7081 catcccggag gatctcctgc tggaagctgc ggtccacttt gagtttcttg aagtacttct
     7141 tgaaatcatg cgactccata tttcccgaag aagcatacgc gtgcgatcct ccggcagtca
     7201 ccaggttctc ttttgtagga tctgcaacag cactcgtggc gactgttccc tggcaaacgc
     7261 aagatgtgct ccccttttca tcgtaggaga ccgcaaactt ggttccgcgc acggccgcaa
     7321 tcgatgttgg ggtggatgct ttgaaacctc gtttggttgg atcatcgact ctaaaccagc
     7381 cgaatccttt ggttacactg acttctccgg gctgactcat gtttgtaagg gacactagag
     7441 tagcatccgt ttctccaagt agacgaatct ccactccttt atagaaaaca atcacctggc
     7501 tttgtctgcc ggtcttgatg cgatcggagt ctttgactac gctgcccact ctcagtgctt
     7561 ctgtcttgcc atctctgagc acagtggcac tgccaatgag gctgcggacc agagcgcggt
     7621 cgttgtctgc gcccagcgga atgagagtta cggatgcagc cgcaagcaag agcaaggatg
     7681 atacgaatct catgcgtttc gtttttggat ttttcaattt acacctctgc acatagccag
     7741 agctcagcgc ggtgtcctgc aatctaaaac gatgaggaag tgcatcccac ggaacaaaca
     7801 gctcgttaga cggaatcaac ggctcgtctg ggatcgcagg caaagcgggc ggacaaaaaa
     7861 gaaaggcgga ctctcgtccg ccacccccga tctcactccc ccgctttcac tgtagaccgc
     7921 atgatggtcc atgcacgatc cactcacagt atgatggcgc taccggcccc cgaatgccag
     7981 aaggcattca tccggtggtt ccctgtcatc caaaacagtg cgcgctctca gctgactccc
     8041 ccggatcctt tcgaatccaa tcagctgccc cctgagcgcc ggcgcttttc gttgtcccag
     8101 accatccggc gctcccccga gctccccctg accgaatgtt ccaggcaact gtcagccctt
     8161 cattgccctg gctgaagaac caccgccccc ggcgatctct gctgtggaag agaggattgc
     8221 gatgaacaaa gatgacgatc catctctttc cccctcaagc aggtgcccga tgactcttcg
     8281 ttgacagacc ctctattgca accagcgtgc caggcagagc acaccctgca tgaacgcctc
     8341 tttttttgta gtaatcagca ctgaatcgtc acatttcaga agattatata gaagaaacct
     8401 gtttgctctt ggcccaagaa cgacgaaaaa gagcgagaac ctccctctcc ctccaagaaa
     8461 atgctagcca atgcctgtcc aaaagctgga atagagcctg ccgtctagaa ttctagatgt
     8521 ataaaaaatt agaaatctac aaatctgaca tgaaatacaa agcccgaact ctctctcttc
     8581 tcctgtttgc ggtatcctgg agtcctgcca tggccgctga tccagtcctg ctcgaggcag
     8641 gacggggagt gtatagactc gggccaaacc tcgaaatact aacggacctg gaagggaagc
     8701 tcaccataga ccagatcaca tccgggaatt ataccagcag cttctatgga tatggctggg
     8761 acagggtccc caacttcgga tacagctccg ctacattctg ggtccgctgc agaatcacca
     8821 acccgcttcc ctttcaaaag gaagtccttc tcgaactggc caacccgctc ctggatgagg
     8881 ccagactctt ctttgtagac gaaggagcat ccagccgaac catcacagaa agcaagagca
     8941 tcaaacatag agtttacatt ttcccggtgc atgttcgccc gcacgaaacc gtagatctct
     9001 acctgcgagt aaaaagcaaa gggtcgattg ctctgcccct cactctattt cgaccagacg
     9061 cgtttgcgcg cgaagaccag gacactcagt tcgcacttgg aatttactat ggcctaatcg
     9121 cggtgatgat cctgtacaat ctgactctgt tcattgcgct tcgcgaacgc gcttacctct
     9181 attattctct cgcactcttc tgcattcacg gcatgcttca gttcgcagtc aacggcctgg
     9241 cacgcaagta tctaccattg tctgcatcgt ggtggagcct gggttccatc ccattctttg
     9301 caagccttgg catattctgg atgcttcttt tctgcagaca ctttttgaac actcgtcttc
     9361 gcacgcctga aattcacaga gtcacaacag cgcttctggt tgtatctctc ttgatgacga
     9421 ttgggtctct ttttatgccc tatcgcgcag ctgtcacaat tcttacgatc cacggccttg
     9481 ctggactgtt ctgcgtgatt gcagccggcg cttacagctg gcgggaatca ttcgcgcctg
     9541 cgcgatacta tttgctcggc tggagtgtag t
//
LOCUS       DAOQJL010000086         9394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3876_length_9394_cov_4.604026, whole genome shotgun sequence.
ACCESSION   DAOQJL010000086 DAOQJL010000000
VERSION     DAOQJL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_9394_cov_4.604026"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLG78_05605"
     CDS             complement(<1..237)
                     /locus_tag="PLG78_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22619.1"
                     /translation="MGLLDRALRFKRPEEKRVPGLLERAKKFKDFVQKDRIPVRETGV
                     VGLFKRALSLRERGGLFARAAKLRGELSEPSGLDF"
     gene            complement(244..1539)
                     /locus_tag="PLG78_05610"
     CDS             complement(244..1539)
                     /locus_tag="PLG78_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22620.1"
                     /translation="MDKKQNQPLELEELDLALPDDLNLGEADVDSGFDDLELPELTAD
                     DDLGELGSADTHAGEEMHSPHDSMTEAEPMDTELHEAAPHPINEDEEPIALSEEELER
                     IVGTKDSELFSDFQEGEHPPEHTDLDFSSEGESDEMQSDLDADISEAHAEPESDFGDM
                     RTDLEDEGPVSLSEEELGNILGDVGESVDMPGEDHHADHADLDMHSDLDSAPAASASA
                     MDESEDESITLSDDELNNILLDSGDLESARGEPKDAPPLSELSMDEEDGPIALTAEEL
                     GNIISDVHEEVEAPEMGEHFDSEMHEEMAAPSLLDEEDEGPVALSDAELDSILEDVHE
                     EVPAEQAGMHADSRANLIVLDEYEDREIAEEKPAIAPQPERFDHVAAEASVPKDDLRK
                     MISYLDQLFDKLPEDTVREFSRSEYFDLYKKIMEDLNLT"
     gene            complement(1648..2043)
                     /gene="atpC"
                     /locus_tag="PLG78_05615"
     CDS             complement(1648..2043)
                     /gene="atpC"
                     /locus_tag="PLG78_05615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNE22621.1"
                     /translation="MKSLHVSLVTPFNSLFEGDVASLQLPLWDGLIGVFPDHAPMLAV
                     IGFGPCTLKLPNGSVESFVLDAGFLEIEKNKVTILASGADYVADINVEKEKKNLAAAH
                     AMEVHSQEDREMKEDRTRSALARIKLAGG"
     gene            complement(2047..3480)
                     /gene="atpD"
                     /locus_tag="PLG78_05620"
     CDS             complement(2047..3480)
                     /gene="atpD"
                     /locus_tag="PLG78_05620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNE22622.1"
                     /translation="MAKQAATKTKAKSGKILQVIGSVLDISFDAGELPDIYTALEIDV
                     PGPDGKKETLTAEIQQHLGGNVCRAVALSSTDGLVRGQEVRNTDAPISVPVGQVTLGR
                     IFNVLGETVDEGPAVQVTERRAIHQKAPDLQNLDSRTEIFETGIKVIDLLAPYTRGGK
                     TGLFGGAGVGKTVVIMELINNIAKAHGGYSVFAGVGERTREGNDLWLEMTESGVLHKT
                     VLCYGQMNEPPGARLRVALTALTMAEHLRDSTGTDCLLFVDNIFRFSQAGSEVSALLG
                     RMPSAVGYQPTLATEMGLLQERITSTRTGSITSVQAIYVPADDLTDPAPATAFTHLDA
                     TTVLSRAISEKGIYPAVDPLDSTSRALQPGVVGEEHYKTAREVQRVLQRYKDLQDIIA
                     ILGMDELSEEDKILVGRARKIERFLSQPFHVAAQFTGRDGIYVKLDDTIRSFKELLTG
                     NYDHLPESAFYMTGTIEQAVANAKAQG"
     gene            complement(3508..4413)
                     /gene="atpG"
                     /locus_tag="PLG78_05625"
     CDS             complement(3508..4413)
                     /gene="atpG"
                     /locus_tag="PLG78_05625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNE22623.1"
                     /translation="MAGAKVLKKRIGAVKNTRKITRTMEMVSTAKAKRMVDRVNAARP
                     YNEKIVEIMKSMESVVGEGAGVLPLLRQVEHPHRVALLVVTANRGLCGGYNASVLRMA
                     RARLVEYQKQGKEVQLFVIGKKGNSYFKFLKVPVKKVITDVDDTFQYDQAKHLSDELI
                     SGFAGAHFDRVEVISTVYYSSALQKPRLDVLLPVGAHTEEDEAELAKKKLTEPVNRAT
                     FLFEPAPAFILKRILPHVIRATVFRKILESVTSEQIARRIAMKNATDNAGSMTKSLTR
                     IYNRKRQAGITQELAEIVSGADAIG"
     gene            complement(4426..5964)
                     /gene="atpA"
                     /locus_tag="PLG78_05630"
     CDS             complement(4426..5964)
                     /gene="atpA"
                     /locus_tag="PLG78_05630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNE22624.1"
                     /translation="MLKIKTDEISSVLKKEIQSFQSRLDLSEVGTVLSVGDGIARVYG
                     LENVMANEMIEFEDGTLGLAFNLEEGSVGVVILGEYTQLREGHQVKRLGRVLEVPVGE
                     SMLGRMVDPMGRPVDDRSLPIHTTKTRPVEFLAPGIARRQPVKEPMQTGIKAIDAMIP
                     IGRGQRELIIGDRGTGKTAIAIDTIINQKGKDVVCVYVAIGQKASKVAAIKDKLTALG
                     CMDYTIIVVATASDPAPLQYLAPYSGASMAEYFMYEKGKATLVIYDDLTKQANAYRQM
                     SLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDKYGGGSLTALPIIETQEGEVSAYIP
                     TNVISITDGQIYLLPNLFASGQRPAVDAGISVSRVGGNAQIKAMRSVAGKMRLDLAQF
                     RDLEAFAQLGTELDAATQAQLDRGYRLLEILKQEENSPWPVEEQVASIFAITRGLMDK
                     IPVNRVREFEKGLIDHLRKAHNEFLKGIREKKAVEDEKALERCIADYATSFLKDQAAE
                     IRPH"
     gene            complement(5957..6526)
                     /gene="atpH"
                     /locus_tag="PLG78_05635"
     CDS             complement(5957..6526)
                     /gene="atpH"
                     /locus_tag="PLG78_05635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNE22625.1"
                     /translation="MIGFEKVAVVYAEALLAAAVDAGPVTELEEDIAQLNELFHKDPD
                     VWAFFKSPIIEVDGKLSLLSKTLKGKVSDTLYSFLCVLCVRRRFELFPDIVEQVKTLL
                     DHHLKRKRVQVFSAVALTPEHSAELQGALKKRLNMDVVLEVHEKPDLIGGLVVRTGDL
                     KEDSSIKDSLARMAKALTGKRILGEKYYA"
     gene            complement(6548..7072)
                     /gene="atpF"
                     /locus_tag="PLG78_05640"
     CDS             complement(6548..7072)
                     /gene="atpF"
                     /locus_tag="PLG78_05640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNE22626.1"
                     /translation="MSATLFIASEGGLSLLSVNPGLAIWTTLTFLLVLGVLHRFAWQP
                     IIQALDERAGKIQSDLDRAEAVRKEAEMKLEDYLQKLNALKEQGTEIIEEAHKDANRI
                     KEELLESAKNEAEAMRQRGVRDIELAKDKALEELHKEVVSLSVGIASQIIGRTLKAED
                     HKRFVEDAIKGLRS"
     gene            complement(7081..7374)
                     /locus_tag="PLG78_05645"
     CDS             complement(7081..7374)
                     /locus_tag="PLG78_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNE22627.1"
                     /translation="MIGLAFIGVGLGAGLAVLGIGVGIGRIGGSATEAISRQPEAVGS
                     IQLAMIIAAALIEGAGLFGLVIAFLMGNQINEKMLNTFAGPAESGKPAATQTK"
     gene            complement(7458..8531)
                     /gene="atpB"
                     /locus_tag="PLG78_05650"
     CDS             complement(7458..8531)
                     /gene="atpB"
                     /locus_tag="PLG78_05650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNE22628.1"
                     /translation="MIRQITRVLTFAAAIAFVPATLHAESPTSEADKFDLGEILVHHL
                     MDAPIFEWNVCVPGNLKIGEYPLCGKKVYEGSEAFEKDPFRRYVFEDKDGKYKWEGGL
                     PMHITRRVMMMFIVVALMLVIFIAGARAIARNPLATKGRYKNSIEAMVQFVRNDIAEP
                     NMHHHSAGFQPYLLTAFFFILFSNMLGLFPPFGELLDMALHGVHPHHGTTASAVTAVW
                     PGITVTGDVAVTMVLAIMTTIQIWITGFRYQGIQFIWTSVPNGVPIWLYPLLWPLEFV
                     ISPLSKGFALTIRLLANMTAGHVIIIVLLGFIMQFKNYWLGFVSVPGAGMIYMLEILV
                     AFLQAFVFTLLSALFIGASMHRH"
     gene            complement(8528..8887)
                     /locus_tag="PLG78_05655"
     CDS             complement(8528..8887)
                     /locus_tag="PLG78_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22629.1"
                     /translation="MRFAISFALYGAIVFWIFQNHPDHAIALCLHSAFGFVLSGAFDA
                     ARIIVSKTNIKRALTIFFVGVFVRMAAVGIYGMLHMFAGTIVPASALISFAGSIVASL
                     LFSVAWEAGWQIYSLRK"
     gene            complement(8887..9192)
                     /locus_tag="PLG78_05660"
     CDS             complement(8887..9192)
                     /locus_tag="PLG78_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNE22630.1"
                     /translation="MQNMGGVIGIGFEFLGIAGFFTAAGIYLDGRFAGRGLILFALVI
                     VGVSAGLWHMIRRVKSIEIEMRAQKKIQKPEKPEDHLRKLDEMTRSLEEMTRKKKDD"
     gene            9191..>9394
                     /locus_tag="PLG78_05665"
     CDS             9191..>9394
                     /locus_tag="PLG78_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22631.1"
                     /translation="MEPKYLQQLAWKADSRPVESLKLKQVAHGWVLSDGKKYATGATR
                     SETWKEWVENPRPLPRPGYFLFQK"
BASE COUNT         2257 a         2463 c         2342 g         2332 t
ORIGIN      
        1 aaaatcaagc ccggacggct cagacaattc cccgcgtaat ttggctgcac gtgcaaaaag
       61 gccgccgcgc tcgcgcaaag aaagggccct tttgaaaagg ccaaccacac cagtctcccg
      121 aaccgggatc ctgtctttct gcacaaagtc cttgaatttt ttcgcgcgtt ctaaaagacc
      181 cggaacgcgt ttttcttccg gcctcttgaa acgaagtgct ctgtcaagta ggcccatgat
      241 ggatcatgtc aggttcaggt cttccatgat cttcttgtaa agatcgaagt actcggatcg
      301 gctgaactcc cgaacagtgt cttccggcag cttgtcaaaa agctgatcga ggtacgaaat
      361 cattttgcga aggtcatctt taggaacgct ggcctctgcg gccacatgat cgaagcgttc
      421 tggttgcgga gcgatggcgg gcttttcttc agcaatctct ctgtcttcgt attcatcgag
      481 aacaatcaga ttcgcgcgcg aatccgcgtg catgcccgcc tgctctgcag ggacttcctc
      541 atgcacatct tctagaatag agtcgagctc tgcgtcagaa agagcaacag ggccttcgtc
      601 ttcttcgtcc agaagagatg gggctgccat ctcttcgtgc atttcagagt cgaagtgttc
      661 gcccatttca ggtgcttcca cttcttcgtg cacgtcagaa ataatattgc ccagttcttc
      721 tgcagtaaga gcgatcggac cgtcctcctc atccatgctg agctcggaaa gaggtggcgc
      781 atcttttggt tctccgcgcg ccgattctag atcgccggag tccaggagta tgttatttag
      841 ttcatcgtct gaaagggtga tgctctcatc ttccgattca tccatggcgg aggcgctggc
      901 tgccggggct gaatccagat cagaatgcat gtctagatct gcgtgatctg catgatgatc
      961 ttctcctggc atgtccacag attcgcccac atcgcccaga atgttgccga gttcttcttc
     1021 agagagagaa acggggccct catcttccag gtctgtgcgc atatcgccaa aatcagactc
     1081 aggttccgca tgcgcttcgc tgatgtctgc gtccagatcg gattgcattt cgtcggattc
     1141 gccctcggat gaaaagtcca gatctgtgtg ctcgggtggg tgttcgcctt cctgaaaatc
     1201 agaaaatagt tcggagtctt ttgtgccgac gatcctctcc agctcttctt cagaaagagc
     1261 gatcggttct tcgtcttcgt ttatcgggtg cggggcggct tcgtggagct ctgtgtccat
     1321 cggctctgct tcggtcatgg aatcgtgagg cgaatgcatc tcttcgcctg catgcgtgtc
     1381 tgccgagccc agttctccca ggtcgtcatc tgccgtgagc tctggcagct ccagatcatc
     1441 aaatccggag tccacgtccg cctccccgag attcaaatca tcgggaagcg ctagatcgag
     1501 ctcttctagt tccagtggct ggttctgttt cttgtccatc ggccaggatt aatccacaaa
     1561 cgaatattca ttatatcatc ggccaggcaa ggccgtaaac gggaaaaaag tggaaaagaa
     1621 tggcgagaaa aggcgaaagg ccggggctta gccgccggcc agcttgatac gggcgagagc
     1681 agaacgggtg cgatcttcct tcatttcccg gtcttcctgg gaatgaactt ccatcgcatg
     1741 ggcagccgcg agatttttct tctctttttc cacgtttatg tctgctacat aatccgcgcc
     1801 gctggcgaga atggttactt tgttcttctc tatttcgaga aagcccgcat ccaggacaaa
     1861 gctttccacg ctgccattgg ggagcttcag agtgcatgga ccaaagccaa tcacagcaag
     1921 catgggagcg tgatctggaa agactccgat cagtccgtcc cagagaggca gctggagaga
     1981 cgccacgtct ccctcaaaga gggaattgaa tggcgtcacc aggctgacgt ggagcgactt
     2041 catgaattac ccctgtgcct tcgcgtttgc tacagcctgt tcgatggttc cagtcatgta
     2101 gaaagcggat tctggcagat gatcatagtt acctgtcagg agttctttga aagaacgaat
     2161 cgtgtcgtcc agtttcacgt agattccatc gcggcctgtg aactgtgctg ccacatggaa
     2221 aggctgggag aggaagcgtt cgatcttgcg agcgcggccc acaagaatct tgtcttcttc
     2281 tgagagttcg tccataccca gaatcgcaat gatgtcctga agatccttgt aacgctgaag
     2341 aacacgctgc acttcgcgcg cagtcttata atgctcttcg cccaccacac ccggctgcag
     2401 agcacgagaa gttgaatcga gcggatccac agcaggataa attccttttt cagaaatcgc
     2461 acgagaaaga accgtagttg catccaggtg agtaaacgca gttgcaggcg ccgggtcagt
     2521 caagtcgtca gcaggaacat aaatcgcctg aacagacgta atcgaaccgg tgcgagtaga
     2581 tgtaattctc tcttgtagaa ggcccatctc cgtagcaaga gttggctggt atcctacagc
     2641 agatggcata cgacccagga gtgcagatac ttcagaaccc gcttgcgaga aacggaagat
     2701 gttatccaca aacaagaggc agtctgttcc agtactatct ctgaggtgtt ctgccatggt
     2761 cagagctgtc agagcaacgc gaagacgagc acctggcggc tcattcatct ggccgtagca
     2821 gagaactgtt ttgtgaagaa cgcctgactc agtcatttcg agccacaggt catttccttc
     2881 acgagttctt tcacctacac ctgcgaatac ggagtaacca ccgtgcgcct tggcgatgtt
     2941 attgatgagt tccatgatca cgacggtttt gcccacaccc gcaccgccga agagaccggt
     3001 cttaccaccg cgagtatacg gagcaagaag gtcgatgacc ttgattccag tttcaaagat
     3061 ctcagtgcga gaatcgaggt tttgcagatc aggagctttc tggtgaatcg cgcggcgttc
     3121 cgtcacctgc accgctggac cttcgtctac tgtttccccg agaacgttga agatacgccc
     3181 cagagttacc tgccctactg gaacagaaat gggagcgtct gtgttgcgaa cttcctgtcc
     3241 gcgcaccagt ccgtccgtgg aagaaagtgc aaccgcacga caaacgtttc cgcccaggtg
     3301 ctgctggatt tcagcggtca gagtttcttt ttttccgtcc ggacccggca catcgatttc
     3361 gagtgcggta tagatatccg gcagttcacc cgcatcgaat gaaatatcca gaaccgaacc
     3421 gatcacctga aggatctttc cgctttttgc ttttgtcttt gttgcagctt gcttggccat
     3481 tttttcttcc tcttgactta tgattgctta accgatcgcg tctgcaccag atacgatctc
     3541 tgccagttcc tgtgtaatgc ccgcctgacg tttgcggttg taaatgcgag tcagtgactt
     3601 ggtcatggaa cctgcattgt ccgttgcgtt tttcatagca atgcgacgcg caatttgttc
     3661 tgaagtcact gattctagaa tcttgcgaaa caccgtagca cgaattacgt gaggcagaat
     3721 tcgcttcaaa ataaaggcag gtgccggttc aaacagaaac gtagcacgat tgactggctc
     3781 tgtcagtttc tttttggcaa gctccgcttc atcttcttct gtgtgagcgc cgactggaag
     3841 aagaacatcc agacgcggct tttgcagggc agagctatag tagacagtag aaatgacttc
     3901 cacacgatca aaatgcgcac ctgcaaagcc agagatcagt tcatccgaaa gatgctttgc
     3961 ctggtcgtac tggaaggtat cgtccacatc agtaatgact ttcttcacgg gcactttcag
     4021 gaatttgaag taggaattcc ctttcttacc aattacaaag agctgaactt cttttccctg
     4081 cttctggtat tccaccagac gagcacgcgc catgcgaaga acgctcgcat tgtatccgcc
     4141 gcagagaccg cggtttgcag tcacaacaag cagagccacg cgatgaggat gttccacctg
     4201 acgcagcaaa ggaagaacgc ctgcaccttc ccccacgacg gattccatgg atttcataat
     4261 ctccacgatc ttctcgttgt aagggcgagc agcattgaca cggtctacca tgcgctttgc
     4321 tttcgcagtt gaaaccattt ccatggttcg cgtgatcttg cgcgtattct ttaccgcgcc
     4381 gattcgcttc ttaagtactt tcgcgccagc cattctcttt cccgattagt gcggacgaat
     4441 ctcagctgcc tggtctttca agaaagacgt ggcgtagtct gcgatgcaac gctcgagtgc
     4501 tttctcgtct tccacagcct tcttctcacg aatgcctttc aggaattcgt tgtgagcttt
     4561 tctgagatgg tcgatgagac ctttttcaaa ttcgcgaacg cggttcactg gaattttgtc
     4621 catgagacct ctggtaatcg caaaaataga agctacctgc tcttctaccg gccacgggct
     4681 gttttcttcc tgcttgagga tttcaaggag gcgataaccg cgatcgagct gcgcttgagt
     4741 cgcggcatcc agttctgttc caagctgtgc aaacgcttct agatcgcgga actgagccag
     4801 gtccagtctc atcttacctg caacagatcg cattgctttg atctgagcgt taccgcccac
     4861 ccggctgact gaaatacccg catccacagc aggtctctgg ccagaggcga agaggtttgg
     4921 cagaaggtag atctgaccgt ctgtaataga aatcacgttg gtaggaatgt aagcactcac
     4981 ttccccttcc tgagtttcaa tgataggaag agctgtgaga gaacctccgc cgtacttgtc
     5041 gctcagttta gctgcgcgct cgagaagacg gctgtggaga tagaatacgt ctccagggaa
     5101 cgcttcgcgg cctggtggac gacgaagaag cagagacatc tggcgatacg cgtttgcctg
     5161 ttttgtaagg tcatcgtaga tcaccagagt ggctttgccc ttttcataca tgaagtattc
     5221 tgccatgctc gctccggagt agggagccag gtactgcaga ggagccgggt cagaagctgt
     5281 ggccacaacg atgatcgtgt agtccatgca acccagggcc gtgagtttgt ctttgatggc
     5341 cgctacttta gaagctttct gaccaatcgc aacgtatacg caaactacat ctttgccttt
     5401 ctggttgatg attgtgtcga ttgcgatggc tgtttttcca gttccgcggt ctccaatgat
     5461 cagttcgcgc tgcccgcggc cgattggaat catcgcgtcg atcgccttga ttccagtttg
     5521 cattggctct tttacaggct gtctgcgtgc aatgccaggt gccaggaatt ctacagggcg
     5581 agtcttggtt gtgtgaattg gaagagaacg atcgtctaca gggcggccca ttgggtccac
     5641 catgcggccg agcatggatt ctccgactgg aacttccaga acgcggccga gtctttttac
     5701 ctgatgacct tcgcgcagct gtgtgtattc gccgaggatt accacaccca cggaaccttc
     5761 ttcgaggttg aatgcaagac cgagtgttcc gtcttcgaac tcgatcattt cgttggccat
     5821 tacgttttca aggccgtata cccgggcaat cccgtcccct acagagagaa cggttccgac
     5881 ttcggaaagg tccaggcgag attgaaagct ctggatttcc ttcttcagta cgctggagat
     5941 ttcatcagtt ttgattttaa gcataatatt tttccccgag aattcgtttg cccgtgaggg
     6001 cctttgccat gcgggcaaga ctgtctttaa tacttgaatc ttccttgaga tcgcctgtgc
     6061 gaactacaag gccgccgatt agatccggct tctcatgcac ttccagcact acatccatat
     6121 tgaggcgctt cttgagcgct ccctggagtt ctgcgctatg ctccggtgtg agagcgactg
     6181 cagagaatac ctgaacgcgt ttgcgtttca ggtgatgatc gagaagagtt ttcacctgtt
     6241 ctacaatgtc cgggaacagt tcaaaacgcc tgcgcacgca gagcacgcag agaaaagaat
     6301 acagcgtatc tgaaactttc cctttgagag ttttggaaag aagactgagt ttcccgtcta
     6361 cttcaatgat gggcgatttg aaaaaggccc agacatctgg atccttatgg aacaactcat
     6421 tgagctgggc gatgtcttct tccagctcag ttacggggcc agcatccacg gcagctgcaa
     6481 gaagagcttc cgcgtacact accgctactt tttcaaatcc tatcatcgaa gcatcctgga
     6541 atcaggttca ggaccgaagc cctttgatgg catcttcaac aaaccgtttg tgatcctcag
     6601 ccttcaaagt ccggccgatg atctgggacg caatgcccac ggaaagggaa acgacttctt
     6661 tatgcagctc ttcgagggct ttatccttcg caagctcaat gtcacgcact ccgcgctggc
     6721 gcattgcctc cgcttcgttt ttcgcagact caagaagttc ttccttgatg cgatttgcgt
     6781 ctttgtgagc ttcctcgatg atttcagtcc cctgctcctt aagagcattg agcttctgca
     6841 agtaatcctc aagcttcatt tcagcttctt tgcggaccgc ttcggcccgg tccaggtcag
     6901 actggatctt acccgctctt tcgtccaggg cctggatgat aggctgccac gcaaagcggt
     6961 gcagcacacc cagaaccagg aggaaagtca gggtagtcca gatcgcaaga cccggattta
     7021 cagagagaag cgaaagaccg ccctcggatg caatgaagag agttgcagac atgaacgtta
     7081 ttacttggtc tgagtagctg caggcttgcc gctttcagcc gggccagcaa acgtattcag
     7141 catcttttcg ttgatctggt ttcccataag gaaagcgatc acaagaccga agagacctgc
     7201 accttcaatc agcgctgccg cgatgatcat agcaagttgg atggaaccaa ccgcttctgg
     7261 ctgacgggag atggcctcag ttgcagaacc gccgatgcgg ccaatgccca cgccaatgcc
     7321 cagtacagca aggccagcgc caagtccgac tccaataaaa gctaaaccga tcatagtttt
     7381 ctcctcctga tcactggcga atcaactttg attcgctctc ttgattgctc tttctaaata
     7441 aattaagaga atccatttca atgcctgtgc atacttgcac cgataaacaa cgcggacagc
     7501 aaggtaaaga caaatgcttg aagaaacgca accagtattt caagcatata gatcatgccg
     7561 gcgccaggca cacttacaaa cccaagccag tagttcttga actgcataat gaagccaaga
     7621 agtacgatga tgataacgtg acccgcagtc atgtttgcca gaagacgaat ggtgagtgca
     7681 aaacccttgg aaagagggga aatcacgaac tcaagaggcc acagaagcgg ataaagccag
     7741 attggaacgc cattaggaac agacgtccag atgaactgaa tgccctggta acggaagcca
     7801 gtgatccaga tctggattgt ggtcatgatg gcaagaacca tggtgactgc aacgtcccct
     7861 gtaacggtga tccctggcca tacagcggtt acagcagagg cagttgttcc gtgatgagga
     7921 tgcactccgt gcagagccat atccagcagc tctccaaacg gagggaacag accgagcatg
     7981 tttgagaaaa gaatgaagaa gaatgcagtg agcagatacg gctgaaaacc cgcagagtga
     8041 tgatgcatgt ttggctctgc gatatcattg cgaacgaact gaaccattgc ctcaattgaa
     8101 ttcttgtatc ttccctttgt agcaagagga ttgcgcgcaa tggccctggc tcctgcgatg
     8161 aagatgacaa gcatcagagc aactacaatg aacatcatca tcacacgacg agtgatgtgc
     8221 atgggaaggc cgccttccca tttgtatttt ccgtctttgt cttcgaagac ataacggcga
     8281 aacggatctt tctcaaacgc ttcgcttcct tcgtagactt tttttccgca gagcgggtat
     8341 tcgccgattt taagatttcc ggggacgcaa acgttccatt cgaaaatggg cgcatccata
     8401 agatgatgaa ctagaatttc tccaagatca aacttgtccg cttcagatgt tggcgattct
     8461 gcatgaagcg ttgccgggac gaatgcaatt gccgcggcga aagtgagcac tcttgtgatt
     8521 tggcggatca ttttcggaga ctatatattt gccacccagc ttcccacgca accgaaaaaa
     8581 gaagactcgc aactatcgat ccggcaaatg aaatcaatgc agaagcaggt acgatggtcc
     8641 ctgcaaacat atgcaacatc ccgtaaatgc caacggccgc cattcgaaca aagacaccaa
     8701 cgaagaaaat cgtcagcgcc cttttgatgt ttgttttaga tacgatgatg cgagccgcgt
     8761 caaaagcacc gctcaaaacg aatccaaacg cagaatgaag gcagagtgca atcgcatgat
     8821 ctggatggtt ttgaaaaatc cagaatacaa ttgcgccata cagagcgaag ctgatcgcaa
     8881 atcgcatcaa tcgtcctttt tcttgcgcgt catttcttcg agagatcgag tcatctcatc
     8941 tagcttgcga agatgatctt ctggcttctc tggcttctga atttttttct gagcgcgcat
     9001 ctcaatctca atcgacttca cgcgtcggat catatgccag agaccggcgg atactccaac
     9061 aatcacaaga gcaaacagaa tcagtccacg gcctgcgaat cgtccatcga ggtaaatgcc
     9121 tgcggcagtg aagaatccgg caattcccag aaattcaaag ccgatcccga tcactccgcc
     9181 catgttctgc atggaaccga aatatctgca gcaactagcc tggaaagccg attccagacc
     9241 tgtggagtca ctcaagctca aacaagtcgc ccatggctgg gtactttcag acgggaagaa
     9301 atatgcaacc ggggcaaccc gatccgagac ctggaaagag tgggtggaga accccaggcc
     9361 gctcccgcgc ccgggttact ttctatttca gaag
//
LOCUS       DAOQJL010000087         9232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_3971_length_9232_cov_5.024082, whole genome shotgun sequence.
ACCESSION   DAOQJL010000087 DAOQJL010000000
VERSION     DAOQJL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3971_length_9232_cov_5.024082"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLG78_05670"
     CDS             complement(<1..1513)
                     /locus_tag="PLG78_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE22632.1"
                     /translation="MNRLKIREASAGTGKTYRIQHDVADFIRMGIRPEKILVVTFTEK
                     ATAELRTKVRDTLAKAPDHPAIRVALSQFDRITVQTIHGFCNQLLGSFAFEMGVSIQP
                     ELTNDRSIRTEVLSSIVRAWPQRFGIDLSDVLSISDYPHFNRADETSTWEQTVLSVSA
                     LYGHGDIILPSSTVSLDASTIAEVRLKLASLRQTASKLVEGSLPGRTEETALRTLIAM
                     PDDLGSLETVLTGAAKKLSKSSLKGAQILAEQAEEIARTLLNMGYYLQKQTILELIEK
                     TNQRKAQRSLISYNDMLEKVRDSLSDSVVEQMRNRYDVAVVDEFQDTDPIQWAVFRRV
                     FLESKDKFLYVVGDPKQAIYGFRGANVHTYYAAARDMEEKGGPDSKGSLIECRRSTPE
                     LIASLNCLFSHNKWFGDRYKNVASMDRPSVPDPMIFVDVSGKLASVAAAKYASHVAYE
                     IQTRVREGAYYRDFCVLVKGKADSNAIERALRRAGIPHAYYKKQGVYQSNEAFE"
     gene            complement(1510..4689)
                     /locus_tag="PLG78_05675"
     CDS             complement(1510..4689)
                     /locus_tag="PLG78_05675"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNE22633.1"
                     /translation="MNRAPESRFFFATDLSTLARKLTDQIAKDKKRDPLQPRRIIVPN
                     RNVARWLEMRLADWSGVCGNVDFQFLEPGIGNRIEEITGKKRALASREDLTLGILECF
                     FEGQVSGALARYIESRNSTDLYHLAAKLASLFQDYEYHRAEWADRWLKGDFAAPAGAH
                     DQAELYFNVMRRMNARGLSNLRGFASFIQEGSAQSEPLILFGVSQISKLHVDILFQLS
                     QLLPLEYYMLDFLQPQKQKWYPCNGLFDEPVGPVRSWLSVQSQQIKILLDQKKENHVW
                     QASLSVPEEASILSTLQRVFTGAPIKKGKEAHSANAGRSPDSSVVIVGCPGVVREAET
                     ILFDIVDRMKEDPTLQLTDIAILVPDMKKYRPALEAVFERDVDAQGRPGIPFSITDFR
                     SGDASLYAAGAHALLDLMDGEFRRSQVFALLRNPCFQHARGISEKTVADWSDYIDRLA
                     VYHGMDPEDPYSFMQGLRRLRLGYVMNLEEGDFAGIVPFHDIFIDEREAGLLSRTIHD
                     LALEIEALRAKAAFEMPEALRKFLEAHLAVPPSRKHERRVQSSVMGMLVRLSHTKVQL
                     PVEIMREIILASASGLSANRGDYLSEGVTISSLQPMRPIPFRITYIAGMEEGSFPGRA
                     DESAINLRNAHPAAGDVSLPEANRMLFLESFLAARDRVFITYNARDTQKDAHFAPCSV
                     VQELIEAAAAIGIQLVPRMVPIHAAVVREGFIPEPESVTALLESVSAGKSAAPDQIEI
                     VKEYLSSRNVSVKHRKNASRRIVSVTRLKAYLEDPVASVLRDAAGMREELEVDSTLDD
                     VEPFYMRSFQYPMMWREAIQAYFQANGQIPVNQIVENLYGNLERRLRVPAGAFGKVQK
                     MQILARLDPSKWQSFTEKKFAPASLSDVSATSGDVSIVGEIPFLRETPGLIEILAPAS
                     NSRARTLLPSYVFFLAWLSARRIDASLTVHVVTVSDGKNPASKSYPFAPLQAQQAQKI
                     LDAIVFGYLSGDVEFVPFSLVERAEPQKRNEGLDPEGYAALLTAILDDPDQGPFTYSR
                     LAALSEARITTRAYDLVRDRLGYLLDGVL"
     gene            complement(4673..5647)
                     /gene="rfaD"
                     /locus_tag="PLG78_05680"
     CDS             complement(4673..5647)
                     /gene="rfaD"
                     /locus_tag="PLG78_05680"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNE22634.1"
                     /translation="MILITGAAGLIGSAVARELNRRGRRDLLLVDHLGTSEKWMNLRS
                     IQYASYIEKDQLLTALQTGSHSLFGINLIFHLGACSSTTERDASYLINNNYQYSIRLA
                     DFAAQKGIRMVYASSAATYGEGENGFEDDLTRVDRLRPLNPYGYSKHMFDLWLRDRRF
                     EPSFAGIKYFNVYGPNEYHKGEMMSVVLKAYRQILADGKIKLFKSYRSDYKDGEQKRD
                     FLYVEDAARMTVYLGLESQALGLFNAGSGGANTWVDLANAIFQAMNRQPQIEFVEMPE
                     HLRAKYQYYTCAPMDTLRSAGYSAPVTPLADAVKDYVQNYLMKDESRA"
     gene            5742..6728
                     /gene="meaB"
                     /locus_tag="PLG78_05685"
     CDS             5742..6728
                     /gene="meaB"
                     /locus_tag="PLG78_05685"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNE22635.1"
                     /translation="MDIDTNALFAGDRRMLARAITLIESTRPEDEMAAEELLHRVLPR
                     IGSSVRIGISGSPGAGKSTFIESFGMKCIEAGSKVAVLAVDPSSSITGGSILGDKTRM
                     SKLGSHESAFIRPSPSGGSLGGVNRRTREVIWLVEAAGFDRILLETVGVGQSEIDAAN
                     MTDFYLLIQLPNAGDELQGIKKGILELADCVFVNKADIDKTLAEKARGELSSALHYQR
                     ARTSGWQIPVLVGSAATGAGLVELMQVIEQFCAHTGRDTGPGSAFHSRRSAQLKEWMH
                     DRIKDKMIRSFYGHPDVQKALPEVEKQVASGALTAQEAMQKLFDVYRLPNAH"
     gene            6746..8956
                     /locus_tag="PLG78_05690"
     CDS             6746..8956
                     /locus_tag="PLG78_05690"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE22636.1"
                     /translation="MQLRSIHLFVLFSFAILGSACSRSPAGRLIVEASPVRQKVGSFE
                     IELTGNQLILSSGSKQFWSTPAGKSFLNAGHGQEKVKESRGSFTINDTLTAFCTDQFV
                     KSTESKPDRLVLRGELACTGPEARVPFQISFSAAGTQLRFEIRVDSHEIEMNRTFLTL
                     NSQRDESIFGFGEQFTYFDLKGREVPVLTQEQGIGRGAQPITIGANITAGAGGDWHTT
                     YIGVPYFISSLGRSFFLENYEYSIFDLRPSDRLTVEVFSSVMTGRISTPGTPLELIEQ
                     YTQYAGRMRPLPDWVHRGAIVGLQGGTDEVKKAIAELDAQRTPIAALWLQDWVGQRTT
                     NFGKQLWWNWELDRDRYPGWDGLVADLRKRDVRVMTYVNPFIANVADKKGNVRRNLFK
                     EAEAKGFLIRHADGSPYLILNTDFSAAMVDLTNPDARKWIKEVIKAELIAGGSSGWMA
                     DFGEALPFDAKLFSGEPASSYHNRYPEEWAMINREAIQEAGKTDIVFFCRSGYTRSPS
                     QATLFWLGDQLVSWDEYDGIKSSVTGLLSGGISGFTLNHSDIGGYTTVNNPIKNYHRS
                     KELFMRWTEMNAFTTVFRSHEGNRPAENHQFNSDAETLQHFARFAKIYAAWFDYRKKL
                     MEEAAQHGWPVIRHLYLEFPDDPAVRSIRYEEFMVGSEILVAPVLDPSAKSVSVYLPR
                     GEWVHLWTGDIKGSADHGSRLTVDAPIGKPAVFYKKGSAVGEAFRKDLATRELL"
     gene            8956..>9232
                     /locus_tag="PLG78_05695"
     CDS             8956..>9232
                     /locus_tag="PLG78_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22637.1"
                     /translation="MEKLTPIQTIQLVLGGLLIAAGVFLGYKTFTKANAILDQPAQLS
                     GWLELRASIHPPAKQEEKSSLLDFKPQMSLIRDEQVYVLGGYLTLFVA"
BASE COUNT         2347 a         2427 c         2268 g         2190 t
ORIGIN      
        1 attcaaatgc ctcgttcgat tggtacactc cctgcttctt gtagtatgcg tgcggaatcc
       61 ccgcccggcg caaagctctc tcgatcgcat tcgagtccgc tttccctttc acaaggacgc
      121 aaaaatctct gtagtatgct ccttcgcgca ccctggtctg gatctcgtag gccacatgag
      181 aagcgtattt ggccgcggca acggaggcta actttccgga aacatccacg aagatcatcg
      241 ggtctggaac agaaggccta tccatagacg ctacgttctt gtatcgatcg ccaaaccatt
      301 tgttatgcga aaacaaacag ttgagcgatg cgatcagctc cggtgtactg cgtctgcatt
      361 ctataaggct tcctttgctg tcaggaccac ctttctcttc catgtcgcgc gcggctgcgt
      421 aatacgtgtg cacgtttgct ccgcgaaaac cgtagatcgc ctgttttgga tcacctacaa
      481 cgtacaggaa tttgtctttg ctttccagga acacgcggcg aaataccgcc cactggatcg
      541 gatctgtatc ctgaaattcg tctacgactg caacatcgta tctgttgcgc atctgctcga
      601 ctacagaatc agacagagag tcccgcacct tttcgagcat atcgttgtag ctgatgaggg
      661 agcgctgtgc ttttcgctga tttgtctttt caatcagttc caggatcgtc tgcttctgaa
      721 gataatagcc catattaagc agagtccgcg caatttcctc tgcttgctct gccaggatct
      781 gcgctccttt caagctggac ttgctgagtt tctttgcggc accggtcaga accgtttcca
      841 gagaccccag atcatccggc atcgcgatga gagtccgcag cgccgtttct tctgttctgc
      901 ctggcaaaga gccttcgacc agttttgacg cggtctggcg cagggaggcc aactttaatc
      961 gaacttctgc aatggtggat gcatcgagag aaaccgtaga tgaaggcaga atgatatcgc
     1021 cgtggccata aagagcgctc acgctcagca ctgtttgttc ccaggtgctg gtttcgtccg
     1081 cccgattgaa atgtggataa tcagaaatgg acagaacatc cgacagatcg attccaaatc
     1141 tctgcggcca tgcgcgaaca atagaagaaa gaacttccgt gcgaatcgag cgatcattcg
     1201 taagctctgg ctgaatgctc actcccattt caaaagcaaa cgaaccaagc aactgattgc
     1261 agaaaccatg aattgtttga acagtgatgc gatcgaattg agaaagagca acgcgaatag
     1321 ctggatgatc tggcgctttc gcaagtgtgt cccgcacttt tgtccggagt tctgcggttg
     1381 ccttttccgt gaaagttacg accagaatct tctcgggacg aatccccatt cgaatgaagt
     1441 cagcgacgtc gtgctgaatc cggtacgttt ttcccgttcc agcggaggct tctcgaattt
     1501 tcagtcgatt cacagcacgc catccagaag atacccgagc ctatctctta caagatcgta
     1561 ggcgcgcgtt gtgatccgag cctctgacaa tgcagcaagc cgcgagtacg taaatggccc
     1621 ctggtcagga tcgtccagaa tggccgtgag caatgccgca taaccttctg gatcaagacc
     1681 ctcatttctc ttttgaggtt ccgccctctc gacgagagag aaaggaacga attccacatc
     1741 tccagaaagg tacccgaaaa caattgcatc tagaatcttc tgagcctgtt gggcctggag
     1801 tggtgcgaat ggataacttt tggaagcagg gttcttacca tccgagacag tcaccacgtg
     1861 aacagtcagg gatgcgtcga tcctgcgcgc ggaaagccat gccagaaaga atacgtacga
     1921 tggcaagagt gtgcgggctc tcgaattact cgcgggcgct agaatttcaa ttaatccggg
     1981 agtttcgcgc agaaatggaa tctcgcctac aatggaaacg tcgccactgg tcgcagatac
     2041 gtctgaaagg gaagcaggcg caaacttctt ttctgtgaaa ctctgccatt tggatggatc
     2101 gagcctggcc aggatctgca ttttctgaac ttttccaaaa gcccctgccg gaacccgcag
     2161 acgtctctcc agattcccgt agagattttc aacgatttga ttcaccggga tctgaccgtt
     2221 tgcctgaaag taagcctgga ttgcttccct ccacatcatc gggtactgaa agcttctcat
     2281 atagaaaggc tctacgtcgt ctaacgttga atcaacctcg agttcttccc gcataccggc
     2341 tgcgtctctg agaacagatg ccacaggatc ctccagatat gccttcaatc tggtgacgct
     2401 tacaattcgt ctggatgcgt tctttctgtg ttttacggat acattcctgg atgataaata
     2461 ttctttaaca atctcaatct gatctggcgc ggcggacttg cccgcagaaa cgctttccag
     2521 aagtgcggtg acactctcag gctcgggaat gaacccttca cgaacaacgg ccgcatgaat
     2581 cggcaccatc cgaggaacca gttgaatccc gatcgcagca gcggcttcta tcaattcctg
     2641 gaccacggaa caaggagcaa agtgcgcgtc cttttgcgta tcccgagcat tgtaagtaat
     2701 aaaaactcga tctctggctg ccagaaacga ctccagaaac agcatcctgt ttgcttccgg
     2761 aagagaaaca tcaccggctg caggatgcgc atttcgtaag ttgatcgcag actcatccgc
     2821 tctacccgga aaactgccct cttccattcc cgcaatatac gtaatccgaa acggaatcgg
     2881 tcgcatgggt tgtagcgaag agatggtcac accttctgac agatagtcgc cgcgattcgc
     2941 gctcagacca gacgcagagg caagaatgat ctctcgcatg atttcaacgg ggagctgcac
     3001 tttcgtgtgc gagagacgaa ccagcattcc cataacagaa ctctgcactc ttctctcatg
     3061 ctttctagat ggaggaacag caaggtgcgc ttccaaaaat tttcgcagag cttccggcat
     3121 ctcaaacgcg gctttcgcgc gaagcgcttc aatctcaagt gccagatcat gaatcgtccg
     3181 cgataacagt cccgcttcgc gctcatctat gaagatatca tgaaacggaa caatccctgc
     3241 aaagtcgcct tcctccagat tcatcacgta cccgagacga agcctgcgaa gaccttgcat
     3301 gaaactgtaa ggatcctccg gatccatgcc atgatacact gcgaggcgat caatgtagtc
     3361 ggaccagtct gccacagtct tctcactgat accgcgcgca tgctggaagc aaggattgcg
     3421 aagaagagca aatacctggc tgcgtcgaaa ctctccatcc attagatcga gtagggcatg
     3481 cgcaccggca gcgtacaggc tggcatctcc agaacgaaag tcggtgatgc taaaaggaat
     3541 tccagggcga ccctgcgcgt cgacgtcacg ctcgaacacg gcttcgagcg caggacgata
     3601 cttcttcatg tcaggaacca gaatcgctat atccgtcagc tgtaacgttg gatcttcttt
     3661 cattctgtcc acgatatcaa agagaatggt ttccgcttcg cgcacaactc ccggacagcc
     3721 aacgatcacg acactcgaat ctggagaacg acctgcattc gctgaatgag cttccttccc
     3781 cttcttaatg ggagcccctg tgaacactct ttgcagagtg gaaagaatac ttgcttcttc
     3841 tggcactgaa agactcgctt gccagacatg gttttccttt ttttggtcca gcagaatctt
     3901 gatctgctga gactgaacag agagccagga acgaaccggc ccaaccggct catcaaacaa
     3961 cccgttacac ggataccatt tttgtttttg aggctgaagg aagtcgagca tgtaatattc
     4021 aagaggtaaa agctggctca gctgaaaaag aatgtccaca tgcagctttg aaatttgaga
     4081 aactccaaaa agaatcaaag gctcagattg cgcgcttcct tcctgaatga aggatgcgaa
     4141 tcctcgcaaa ttggacaaac cccttgcatt cattcgccgc ataacgttaa aatacaattc
     4201 tgcctgatca tgtgcaccgg ccggagctgc aaaatccccc ttcagccacc gatcggccca
     4261 ttccgctctg tggtattcat agtcctgaaa gagagacgcc agttttgctg ctagatgata
     4321 aagatccgtg ctatttctgg attctatgta cctggccagg gcaccggaaa cctgaccttc
     4381 aaaaaagcat tccagaattc caagggtcag atcttctcta ctggctagag ccctcttctt
     4441 ccctgtgatt tcttcgattc tattgccaat gcctggttcg agaaactgga aatcgacatt
     4501 tccacagaca ccactccagt ctgcaaggcg catctccagc cagcgcgcga cgttcctgtt
     4561 tggaacgatg atgcgcctgg gctgcaatgg atcgcgcttc ttatccttcg cgatttgatc
     4621 tgtaagtttg cgcgcaagcg tacttagatc tgtggcgaag aagaatctcg attcaggcgc
     4681 gcgattcatc cttcatcaaa taattctgaa catagtcctt cactgcatcc gcaagaggag
     4741 tcactggagc actataaccg gcggatcgaa gagtgtccat gggcgcgcag gtatagtact
     4801 gatactttgc tctgaggtgc tccggcattt caacaaactc tatttgtggc tgcctgttca
     4861 tcgcttggaa gatagcattc gcaaggtcca cccaggtgtt cgcgccacca cttccagcgt
     4921 tgaacaatcc cagtgcctga gattcaagcc ccagataaac agtcatcctg gccgcatcct
     4981 ccacgtagag gaaatcacgc ttttgctccc catccttgta gtccgatcta tagctcttga
     5041 agagcttgat cttgccgtcc gccagaatct gtctgtaagc tttcagcacc acagacatca
     5101 tctctccttt gtggtattca ttgggtccgt atacattgaa gtacttgatg cccgcaaaag
     5161 aaggctcaaa acgacgatcc cgcaaccaca ggtcgaacat atgcttggag tagccatacg
     5221 ggttcaaagg gcgaagacga tctacgcgcg tcaggtcatc ctcgaatcca ttttcccctt
     5281 ccccatatgt ggctgcactc gacgcataga ccatccgaat gcctttctgg gccgcgaaat
     5341 ccgcaagtct tatagaatat tgatagttat tgtttattaa ataactggca tcgcgctccg
     5401 tggtggaact gcaagcaccc aggtggaaga tcagattgat tccaaacaaa ctatgtgacc
     5461 ccgtttgaag cgcggtgaga agctggtctt tctcaatgta gctcgcatat tgaatggatc
     5521 tcagattcat ccatttttcc gaagtgccca gatgatccac gaggaggagg tctcttctac
     5581 cacgacggtt tagttcgcgg gccacggcac tcccaatcag gcccgcagct cccgtaatca
     5641 gaatcatgcc agattgaatt tgcgggcgtc gacagagtca acctcaaaag gtttttcggt
     5701 tgcctggcaa aggcaatccc cgagcctgaa gcgggctggc aatggacatc gacacgaatg
     5761 cactcttcgc aggtgatcgg cgaatgctgg cccgcgccat cacgctaata gaaagcacgc
     5821 gaccagaaga tgaaatggca gccgaagagc ttctgcaccg cgtgctaccg cgaatcggtt
     5881 cctctgttcg cattggaatc agcggctctc caggagccgg aaaatctaca ttcatcgaat
     5941 cgttcggaat gaagtgcatt gaggcaggaa gcaaagtcgc tgtgctcgcc gtagatccaa
     6001 gttcaagcat cacaggtgga tccatacttg gagacaaaac acgaatgtca aaactcggca
     6061 gccatgagag cgccttcatt cgacccagcc catcgggtgg atccctgggc ggagtgaatc
     6121 gccggacacg cgaagtgatc tggcttgtgg aagcggcagg gtttgacagg attctactcg
     6181 aaacagtcgg agtaggacaa tcggaaattg acgcggccaa tatgacggac ttctatctgc
     6241 tcatccagct gccaaacgca ggagatgaac tgcagggaat caagaaagga attctggaac
     6301 tcgccgattg cgttttcgtg aacaaggcag acatagacaa aacacttgca gaaaaagcgc
     6361 gtggagaact ctcctctgcg ctccattacc agcgtgcgcg cacatcgggc tggcaaattc
     6421 cagtgcttgt gggaagcgct gccacaggcg cgggactggt ggagctgatg caggtgattg
     6481 agcaattctg cgcccatact gggagagaca ccggaccagg aagtgcattt cattctagaa
     6541 gatccgctca actgaaagaa tggatgcacg atcgcatcaa agacaaaatg atccgcagct
     6601 tctacggtca tcctgatgtt caaaaggcgc tgccggaagt agagaaacaa gtcgcttcgg
     6661 gtgcacttac agctcaagaa gcaatgcaaa aactatttga cgtctaccgc ttgccgaacg
     6721 ctcattaggc gctttcgggg gcttcatgca attacggtcc atccatcttt ttgtgctctt
     6781 ttcatttgcg attctgggtt ctgcgtgctc tcgctcgccg gccggaaggt tgatcgtgga
     6841 ggcatctcca gtacgacaga aggtaggctc tttcgagata gaactcactg gaaatcagct
     6901 gattctttcc tcgggctcaa aacaattctg gtctacgcct gccggcaaga gcttcctgaa
     6961 tgcaggtcac gggcaggaga aagtaaagga atccagagga tctttcacga tcaacgatac
     7021 gctcaccgca ttttgcacgg atcaatttgt aaagtccacg gaatcaaagc cggaccgact
     7081 cgtgctgcgc ggagaactcg cgtgcacggg accggaagca cgcgttcctt tccaaatctc
     7141 tttttcagcc gcgggcacac aactgcgatt tgaaattcgc gtggacagtc atgaaatcga
     7201 aatgaacaga actttcctca ctctaaattc acaacgagat gaaagcattt ttggttttgg
     7261 agaacaattt acatatttcg atctcaaagg gagagaagtc cctgtgctca ctcaggaaca
     7321 gggaattggt cgtggagcgc agcccatcac aatcggagca aacatcaccg caggcgcggg
     7381 aggagactgg cataccacct acatcggtgt gccttatttc attagctcgc ttggcagatc
     7441 cttttttctc gaaaactacg aatactccat attcgatctc cgtccgtctg atcgcctaac
     7501 ggtagaagtt ttttcttccg ttatgacagg cagaatctct actccgggaa ctccactgga
     7561 attgatcgaa cagtacacac agtacgccgg tagaatgaga cctcttccag actgggttca
     7621 ccgcggagcc atcgttggcc ttcagggcgg cacagacgaa gtcaaaaaag caatcgcaga
     7681 actggatgca cagagaacgc ccatcgcagc actctggttg caggactggg tgggccagag
     7741 aactaccaac tttggcaagc agctctggtg gaactgggaa ttggaccggg atcgatatcc
     7801 aggatgggac ggactggttg cagatctaag aaaacgcgat gtccgcgtga tgacttacgt
     7861 aaatcctttc atcgcaaatg ttgcggataa aaaaggaaac gttaggcgca atctattcaa
     7921 ggaagcagaa gcaaaaggat tcctgatccg ccacgcagac ggatcgcctt atctgattct
     7981 gaatacggat ttttccgcag ccatggtgga cttaaccaat ccggacgcgc gtaaatggat
     8041 caaagaagta atcaaagcgg aactcatcgc aggtggttcg tccggatgga tggcagattt
     8101 tggcgaggca cttccatttg atgcgaagct cttttccggg gaaccggctt cctcgtatca
     8161 caatcgctat ccggaagaat gggccatgat caaccgcgag gccattcaag aggcaggaaa
     8221 aactgacatt gtcttctttt gtcgctccgg ctatactcgc agcccatctc aagccactct
     8281 gttctggctg ggtgaccaac tggtaagctg ggatgagtat gacggaatca aatcctccgt
     8341 aacaggtttg ctcagcggtg ggatatctgg tttcactctg aaccattcgg acatcggcgg
     8401 ttacaccacg gtcaataacc cgatcaagaa ctaccatcgc tccaaagaac tattcatgag
     8461 atggacagaa atgaatgcgt ttactacggt ctttcgctct cacgaaggca atcgacctgc
     8521 agaaaatcat cagttcaatt cggatgcaga aactttgcag cactttgccc ggttcgcgaa
     8581 gatttacgct gcctggtttg actaccgcaa gaaattaatg gaagaagcag cgcagcatgg
     8641 atggcccgtt atacgacatt tatatcttga attcccggat gatcccgctg tcagatccat
     8701 acgctatgaa gaattcatgg tgggctctga aattctggtg gctcctgtac tggatccttc
     8761 tgcgaaatca gtttcggtgt atttgccgcg tggagagtgg gttcatctct ggacaggaga
     8821 tataaaaggg tctgcagatc acggaagtcg cctgacggta gacgcgccga tcggaaagcc
     8881 cgcggtgttc tataaaaaag gctcagccgt aggagaagcg ttcagaaaag acctggccac
     8941 aagggagctg ctttagtgga aaagttaaca cccatacaga caattcaact ggtcctgggc
     9001 ggcctattga tcgcagcggg cgtctttctt ggctacaaaa cgtttacaaa ggcaaatgca
     9061 atcctcgatc aacccgctca actctctggc tggctggagc ttcgcgcttc catccatcca
     9121 cctgcaaaac aagaagagaa atcttccctg ctcgacttca agcctcagat gtccctgatc
     9181 agagacgagc aagtctacgt gctcggggga tatctgacgc tgtttgtagc gg
//
LOCUS       DAOQJL010000088         9178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4016_length_9178_cov_4.746136, whole genome shotgun sequence.
ACCESSION   DAOQJL010000088 DAOQJL010000000
VERSION     DAOQJL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_9178_cov_4.746136"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..932)
                     /locus_tag="PLG78_05700"
     CDS             complement(9..932)
                     /locus_tag="PLG78_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE22638.1"
                     /translation="MKYESIHAIVNPASSGGEAGRRWPETQKRMSSVLKNFSFELTSA
                     PGDGERLASAAIHEGVQSIVVIGGDGTLSEVVNGVLHFSESQRPRIGIINHGTGGDFA
                     RTIGAPTDIQLVLEKILSARDHAVDAGRITFTNHQGVQAKRYFVNVAGCGLAGEVVKA
                     INSSSRRFGAFSYFSTSFTNAITYKNAKVRISLDGGPETTETITTLAICNGQFFGGGM
                     QIAPDASITDGLLDVTILGNWSLAQKVRYSRNLYNGTISHCYGVKTARVKTIRIEPDN
                     LNPVLIDCDGEAVGKAPLTAEVMPGALRLIL"
     gene            1013..2041
                     /locus_tag="PLG78_05705"
     CDS             1013..2041
                     /locus_tag="PLG78_05705"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22639.1"
                     /translation="MSRRNRTRLLSVIIASFVSALIGFVYSFTFNQSIFGGIVTGFTM
                     GFVVLSFEWFVLQGRYAHRTRNVSPPVFLLISLVCWTILLFTILRISHSLFAQPGDVI
                     PMWSWRTARGMILGVVMISGINLFLRTKYLVGMRVLGNFFLGRYNRPVQEDRIFMFLD
                     LEGSTAISERLGALRTHKLIRRFFQDVAEHVYEFGGETHRYVGDEVVVTWPTNQGLKN
                     AAWVNCIFDIRQAIDAERFSYYEEFGVMPEFRIGLHCGEVVAGEVGEDKREIVYIGDT
                     VNTAARLEQLCREKGTWLLVSEDILSRTELPSHIESKSLGPVTLRGRSKSMEVFTLRP
                     MKKLDSET"
     gene            complement(2062..2904)
                     /locus_tag="PLG78_05710"
     CDS             complement(2062..2904)
                     /locus_tag="PLG78_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNE22640.1"
                     /translation="MSLAELIAGIPENPRSRIAPTPSGLLHRGNAFNFLLTWTLVRQK
                     QGTLHLRIDDLDSARRRKEYIEDIFESLHWLGIDWDSGPRNSTEQQNIFSQDLRLRRY
                     HDLIQRLIDHGHCFACTCSRTETGPVYPGTCEAKNLPLDEPGTSIRLRVAQGEIVQIK
                     DRVTTSFAIGPETGSFVIRRKDGLPSYQIASVSDDEDMQMNLIVRGMDLLPSTAMQTL
                     IAEKAGLLNFKNASFIHHSLITKDGEKLSKSAGAESLRVLRQGGEEAAKSMRREFERW
                     ANAL"
     gene            2987..3811
                     /locus_tag="PLG78_05715"
     CDS             2987..3811
                     /locus_tag="PLG78_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22641.1"
                     /translation="MFFQESIFRSKRVLVTGGGSGIGLCIARDFLAHGASVCILGRDE
                     EKLSRAAADLKASGNVFTVSCNLRSDEEIPRAMDELASALGGLDILVNNAGGQFPGGT
                     EQISAKGFRAVVDTNLTGTFLITKACFERFLKKSGGSIVSILMDMRNGMPLMPHSAAA
                     RAGVDNLTKSWAAEWAHFGVRVNSVAPGIIRSSGLDTYAPEFRKIVEEMALKNYAYRA
                     GTEEEVSAAVLFLSSSLASYITGQTLFVDGGESIYSPFLPPRPHDRLPAYLQNKRN"
     gene            3867..5978
                     /locus_tag="PLG78_05720"
     CDS             3867..5978
                     /locus_tag="PLG78_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNE22642.1"
                     /translation="MGKNSNTIESLFGQNVFNQHVMKTMLPKETYRAFKRVLEQGQPL
                     TAAQADDIAAAMKDWAVEKGATHFTHWFQPMTGFTAEKHDSFIKPTDDGRAMAEFSGK
                     ELIKGEPDASSFPSGGLRATFEARGYTAWDISSPAFLKEDEGGLTLCIPTAFCSYTGE
                     ALDKKTPLLRSMDALSREAVRVLRLIGNTQVQKVTTTVGAEQEYFLIDREYYEQRPDL
                     IFAGRTLFGAMPPKGQEMEDHYFGSIKDRIAGYMRDLDRELWKLGVSAKTKHNEVAPA
                     QHELAPIFQTTNIAADHNQLTMEMMRKVAIRHDLVCLLHEKPFAGVNGSGKHNNWSMS
                     TDDGQNLLDPGHTPHENVQFLLFLSAVIKAVDEYGDLLRVSAANPGNDHRLGANEAPP
                     AIISVYLGDQLGHILDQIDAGDAKESKRGGILDSGTPMLSTLPKDSSDRNRTSPFAFT
                     GNKFEFRMVPASISISAPNFTLNTIVAEALSGIGDRLEKATDRRAEVKTIIQEIVRKH
                     KRVVFNGNNYAKEWVAEAAKRGLPNISSTVEAFKSIVAEKNVALYEKHKVFTRTELEA
                     RYEISLENYSKTIRIEALTMVDMARRMIQPSVIRYATFLAESVQTVKAAGADVSVQEG
                     LLKSVSSLGVQFNEKLGALEKAIEKADATHGSSQAHAEAYRDVVFTAMGALREVGDKL
                     ETIVDESFWPMPTYGDLLFRV"
     gene            complement(6020..7543)
                     /locus_tag="PLG78_05725"
     CDS             complement(6020..7543)
                     /locus_tag="PLG78_05725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22643.1"
                     /translation="MDENRYQELLSCIETLESWAGNPDDFTQLPEEVRVRLIRVAGQI
                     ARPNRLEQKNRNKALRRAKRSAIVAQERSARAATGIRDARRATVFKAPTQIEGPSHDS
                     APELSSPRNCYVCNAEYTKLHFFYDSMCQTCGDFNYQKRFQTAPLGDQVALITGARLK
                     IGYQAALMMLRAGARVIVTTRFPVDAANRYSKEDDFASWSDRLQIFGLDLRHTPSVEL
                     FAQYFEQTYDRLDILINNAAQTVRRPPGFYAHLMENETAGLSSIDEKARTLLSAHEDC
                     LRHLNLFSETNLATEKALPVTWNGRLPGIGLTSSARLSQIPYTHDQSLVAEQVFPAGK
                     LDADLQQVDLRETNSWRLRIGEIPTAEMIELQLINAIAPFVLCNRLAPMMQKNPTGQK
                     HIVNVTAIEGKFYRFRKPDRHPHTNMAKAALNMLTHTSASGLAKHGIFMNAVDTGWVT
                     DEDPAILAKHKQDVHDFEPPLDIVDGAARVCDPFFHGILTGQHWCGKFLKDYQPIDW"
     gene            7600..>9178
                     /locus_tag="PLG78_05730"
     CDS             7600..>9178
                     /locus_tag="PLG78_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22644.1"
                     /translation="MPHGSAAFASVVVTVAVSRNKLLARIRTVFIPRRPDIRFFSEEL
                     HYQSSRVAGFGAFLLLFVCLPYLWLDPLIHPGSPWMLVLRIGQSLMGLIALVAVFFDR
                     VRKNAMYLVFVNLLYVELSGAVIAGLTGGDPVYTGGFALVLMITCLVPVPRMYALLTA
                     FASVAVYAWFMVSAKLDVSIPRNIFALQLIVTSLSITVFFIFILDRIRHSSFEKTLAI
                     QAARDDILKLSEFAQKANQSIDLNKILDEIIAYVQENFSIEGTLLQLYDPKTDELVYF
                     KTNFQDFHDEDLRKYVQALRFSAGKRSSAGAILRRGKPMYFPNMKVARRNENQDPLDH
                     FVVRAGIESALIVPLIVADAPAGMLYFSNYHGDMNLSRDQLSTIYAFCQQIGGAIHSS
                     SLIHQVEREKEKSERLLLNILPESVALELKENGFVRPTMFENVTILFTDFKGFTHHAA
                     SMSPQDLVTELDGIFGQFDAIAEKYGLEKLKTIGDSYMCVGGLPKSNRTHPIDACLAG
                     LEMAAFVKQANELRLAST"
BASE COUNT         2191 a         2263 c         2346 g         2378 t
ORIGIN      
        1 ggtttctctc agagaatgag gcgaagcgca cctggcatca cttctgcagt aagtggcgct
       61 ttgcccaccg cttctccgtc gcaatctatc aggacagggt tcagattgtc tggttcgatg
      121 cggatggtct tcactctcgc cgtcttgact ccgtaacagt gagagattgt gccgttgtac
      181 agattgcgag agtagcgcac tttctgtgcg aggctccagt ttccgagaat cgtcacatcg
      241 agtagtccgt ctgtgatgct tgcatcgggc gcgatttgca ttccgcctcc gaagaactga
      301 ccattgcaaa tcgcaagagt tgtaatagtc tccgttgtct ccggtccacc atccaggctg
      361 attcttactt tcgcattctt gtatgtgatc gcgttggtga atgatgttga aaaatagctg
      421 aacgctccga atcgacgtga gcttgaattg attgctttca caacttctcc agccagaccg
      481 catcccgcca cattgacaaa gtatctcttt gcctggacac cctgatgatt cgtgaacgtg
      541 attctacctg cgtccactgc atgatcgcgc gcagacaaaa tcttttcgag cacgagctgg
      601 atgtccgttg gcgcaccgat ggtgcgagca aagtcaccgc cagttccgtg attgatgatt
      661 ccgattctgg gtcgctgtga ttcagagaaa tgaagaacgc cgtttaccac ttcggaaaga
      721 gttccgtcac cgccaatgac gacgatgctt tgaacaccct catgaatcgc tgcagatgcc
      781 aatcgctcac cgtctcctgg tgcgctggtg agttcaaaac tgaaattctt cagcacagaa
      841 ctcattcttt tctgggtttc gggccagcgc ctcccggctt cacccccgct ggatgctgga
      901 ttcacgatgg catggatgga ttcgtatttc atgaaagggg tagacaatcg agatcccggg
      961 gaacacaaat gtaaactact tttcggcgcg acagccactc gtggtcagaa gaatgtccag
     1021 acgcaaccgc accagactgc tctctgtaat aatcgcgagc tttgtctctg cgctcatcgg
     1081 gttcgtttac agtttcacat tcaatcaatc catattcggc gggatcgtca ccgggttcac
     1141 catgggattt gtggttttgt ctttcgaatg gtttgttctt cagggtagat acgctcaccg
     1201 gacgcgcaat gtctcccctc cagtgtttct attgatttcc cttgtttgct ggactattct
     1261 gctctttacc attcttcgca tttcgcacag tctctttgcg cagccaggtg atgtaattcc
     1321 gatgtggtcg tggagaactg cacgaggaat gattctcggc gtagtgatga ttagcggaat
     1381 caatttgttt cttcgcacaa agtatctcgt gggtatgcgc gttctcggaa acttttttct
     1441 gggaagatac aatcgtcccg tccaggaaga tcgcatcttc atgtttctgg atctagaagg
     1501 atctactgcg atttcagaaa gactcggggc ccttcgcact cacaaactca ttcgccgttt
     1561 ctttcaggac gtggcagagc atgtctacga attcggagga gaaacacata gatacgtggg
     1621 cgatgaagta gttgtgacat ggccaacgaa tcagggtttg aaaaacgctg cctgggtgaa
     1681 ttgcatcttt gatattcgcc aggcgattga tgcagagcgt ttctcttact atgaagaatt
     1741 tggagtcatg ccagaatttc gcattggact tcattgcgga gaagtggttg cgggagaagt
     1801 aggagaagac aagagagaga ttgtttacat aggagacaca gtcaacactg cggccaggct
     1861 agaacaactt tgcagagaaa agggaacctg gcttcttgtt tcggaagaca ttctatcacg
     1921 cactgagctt ccttctcaca tcgagtccaa gagcctgggg cccgttacat tgcgcggaag
     1981 atccaagtcc atggaagtat tcactctgcg tcccatgaag aaattggatt ctgaaacctg
     2041 agcgcgaatc tctgctttgc gtcaaagtgc attcgcccat ctttcgaatt cacgacgcat
     2101 gcttttagcc gcttcctctc cgccctgcct gagcactcga aggctttctg ctccagcgga
     2161 ctttgaaagc ttttcgccgt cttttgtaat gaggctatgg tgaatgaaag aggcgttctt
     2221 gaagttcaag agccctgcct tttctgcgat cagggtttgc atggctgtag aaggcaaaag
     2281 atccattcca cgaacaatta gattcatttg catatcttca tcgtctgata cggaagcgat
     2341 ctggtaagaa ggaagtccgt cctttcttct gatcacaaag cttcctgtct ccgggccaat
     2401 cgcaaaactg gttgttacgc gatccttgat ctgaacgatt tcaccttgtg ccacgcgaag
     2461 gcgaatggat gtgcctggtt catccagggg caggttcttt gcttcgcacg ttcctggata
     2521 caccggtcca gtttcggttc tactacacgt gcaggcgaag caatgcccat ggtcgatcaa
     2581 cctttgaatc agatcatgat agcggcggag tctcaaatct tgagagaaga tgttttgctg
     2641 ttccgtagaa tttcgggggc cggagtccca gtcgattcct agccagtgca acgattcgaa
     2701 aatgtcttcg atgtattctt tgcgccttcg agcagagtcg agatcgtcta tgcgcaggtg
     2761 cagtgtgcct tgtttttgtc ggaccagagt ccatgtcagc aggaagttga aagcgtttcc
     2821 tctatggagg agtccgcttg gggtgggagc gattctgctt cttggatttt caggaatgcc
     2881 agcgatcagt tccgcgagac tcacgacaca gttccgatct gcggatctct ggtgcaagaa
     2941 tcgttttttt tgcttttctc agttctggtg gctgccacga tcacgcatgt tctttcaaga
     3001 aagcatcttt cgctcaaagc gagtactcgt gaccggggga ggatccggca taggcctttg
     3061 catcgcacgt gacttcctcg cgcacggagc ctctgtttgc attctcgggc gagacgaaga
     3121 aaagctgtcc cgtgctgccg ctgatttgaa agccagtgga aatgttttca ccgtgagttg
     3181 caatttgaga tcggatgaag aaattccccg cgcgatggac gaacttgcaa gcgcgctcgg
     3241 tgggcttgac attctggtga acaatgcagg cgggcaattt cctggaggga cggaacagat
     3301 ttctgcgaaa ggatttcgcg cagtcgtaga tacaaacctg acgggtacat tcttgattac
     3361 gaaggcctgc tttgagcgat tcttgaaaaa gtccggtgga tcgatcgtga gcattctcat
     3421 ggatatgaga aatggaatgc cgctgatgcc gcactccgcg gcggcgcgcg caggagtgga
     3481 caatcttaca aaatcgtggg ctgccgagtg ggctcatttt ggcgtgcgcg tgaattctgt
     3541 tgcacccggc atcattcgat cgagtggtct ggatacgtac gcacctgaat ttcgcaaaat
     3601 agtggaggag atggctctta agaattacgc gtatcgcgca ggcacggaag aagaagtaag
     3661 cgcggctgtt ttgttcctct cctcgtcact tgcctcctac atcacggggc agacgttgtt
     3721 cgtggacggc ggagaatcca tttattcgcc tttcttgccg ccacggccgc atgatcgcct
     3781 tccagcatac ttgcaaaata agagaaacta agatttgacc gcctcgtaaa ttgtggtcct
     3841 ctggcaaaaa gacttcggag acccctgtgg gaaagaattc aaacacaatc gaatcgttat
     3901 tcggacagaa cgtatttaat cagcatgtaa tgaagacaat gctgcccaaa gaaacatatc
     3961 gcgcattcaa acgcgttctc gagcagggtc aacctctgac tgcagctcaa gcggatgaca
     4021 ttgcagccgc aatgaaagat tgggcagttg aaaaaggcgc aacacacttc actcactggt
     4081 tccagccaat gactggcttt acagcagaaa aacatgattc attcatcaag ccaaccgatg
     4141 acggtagagc tatggcggag ttttcaggaa aagaactgat caaaggagag ccagacgctt
     4201 cttccttccc gtcaggcggt cttcgcgcta cgttcgaagc acgtggttac accgcatggg
     4261 acatcagctc gcctgctttc ctcaaagaag acgaaggtgg actcacgctc tgcattccca
     4321 cagcattctg ttcttatact ggagaggctc tggacaagaa aactccactg cttcgctcca
     4381 tggatgctct ttcgcgagaa gcggttcgtg tgcttcgtct gattggcaac actcaggtgc
     4441 agaaagtaac cacaactgtg ggagcggaac aggaatactt cctcatcgac cgcgaatact
     4501 acgaacagcg tcctgacctt atctttgctg gccgtacact ttttggagcc atgcctccga
     4561 aaggccagga aatggaagat cattactttg gaagtatcaa ggatcgcatc gcgggctaca
     4621 tgcgcgatct ggatcgtgaa ttgtggaagc tgggcgtttc agcaaaaaca aagcacaacg
     4681 aagtagcacc tgcacagcac gaactggcac ccatcttcca gaccaccaac attgccgcag
     4741 atcacaatca gctcacaatg gaaatgatgc gcaaagttgc gattcgccac gaccttgtgt
     4801 gtctgctgca cgaaaaacca ttcgcgggcg tcaacggttc cgggaagcac aacaactggt
     4861 ccatgtctac agatgacggc cagaaccttc tcgatccagg tcatacacca cacgaaaatg
     4921 tacagttcct gctgttcttg agcgctgtga tcaaagcagt ggatgagtat ggagatctcc
     4981 ttcgcgtctc cgcggctaac ccgggaaacg atcatcgcct tggtgcaaat gaagctcctc
     5041 ctgcgatcat ctctgtttat ctcggagatc agctgggaca catcctggac cagatcgacg
     5101 cgggagatgc gaaagaatcc aagagaggcg gaatcctgga cagcggaact ccaatgcttt
     5161 ctacactgcc caaagattct tctgacagaa accgtacatc accgtttgca ttcactggaa
     5221 acaaattcga attcagaatg gtgccagcat cgatttctat ttctgctcca aactttacgc
     5281 tcaacacaat tgtagcggaa gcgctttctg ggattggaga tcgtctggaa aaagcaacgg
     5341 accgtcgtgc agaagtcaaa acgatcattc aggaaattgt gcgtaagcac aaacgtgtgg
     5401 ttttcaacgg aaacaactat gcgaaggaat gggttgccga ggcagcaaaa cgcggtcttc
     5461 caaacatctc cagcaccgta gaagctttca agtccatcgt cgcagagaag aacgtggctc
     5521 tctatgagaa acacaaagta ttcactcgca cggaactgga agcgcgctac gaaatcagtc
     5581 tagaaaacta cagcaagacc attcgcatcg aagctctgac aatggtagac atggctcgtc
     5641 gcatgattca gccttcagtg atccgttatg cgacattcct tgctgaatca gttcagactg
     5701 taaaagcggc cggcgctgac gtatccgttc aagaaggact gctcaagtct gtatcttctc
     5761 tgggcgtgca gttcaatgag aagctcgggg ctctggaaaa ggccatcgaa aaggcagacg
     5821 caacgcacgg gtcttcgcaa gcgcacgcgg aagcctatcg tgacgttgtt ttcacagcca
     5881 tgggcgctct gcgtgaggtt ggagacaaac tcgaaacaat cgtcgatgaa tccttctggc
     5941 caatgccaac ctacggagac cttctcttcc gcgtttaaca aagtgagaca atgtgcgacg
     6001 gccgttctgg ccgtcgcatc taccaatcga ttggttgata atctttaaga aatttcccac
     6061 accagtgctg gccagtcagg attccgtgga agaatggatc acagactcga gcggctccat
     6121 ccacaatatc cagaggtggt tcaaaatcgt gtacgtcctg tttgtgtttc gcgaggatag
     6181 caggatcctc atccgtgacc catcctgtgt ctactgcgtt catgaaaata ccatgtttgg
     6241 ccaatccgct tgctgaagtg tgggtcagca tgttgagtgc tgcttttgcc atgttcgtgt
     6301 gcggatgacg gtctggtttc ctgaaccgat aaaattttcc ttcgatcgca gtcacgttca
     6361 ctatgtgttt ttgccctgta ggatttttct gcatcatagg agccaggcgg ttgcacagta
     6421 caaagggagc gattgcgttg atcaattgaa gctcgatcat ttccgcggtt gggatttcac
     6481 cgatgcgaag acgccagctg ttggtttcgc gaagatctac ctgttgtaaa tcagcatcga
     6541 gtttgccggc cggaaacacc tgctccgcta caagggactg atcgtgagtg tagggtatct
     6601 gtgaaagcct ggcagaagaa gtgagtccta tacccgggag tcttccgttc caggtgacgg
     6661 gaagagcctt ctcagtggca agattggtct ctgaaaatag attcaaatga cgcaaacaat
     6721 cttcgtgcgc agaaagcagg gtgcgcgcct tctcgtcgat agaagaaagc ccggcggttt
     6781 cattttccat cagatgtgcg tagaatcccg gaggacgtct cacggtttgc gcggcgttgt
     6841 tgatcagtat gtccagccga tcatacgttt gctcaaagta ttgagcgaag agctcaacgc
     6901 tcggtgtgtg acgtaagtcc agaccgaaaa tctgcaagcg atccgaccag gatgcaaaat
     6961 catcttcctt gctgtatcga tttgctgcgt cgacggggaa gcgggtagtg acaattacgc
     7021 gagcgcctgc gcgaagcatc atcagcgcgg cctgatatcc aatcttcaat cgtgcgccag
     7081 taatgagtgc cacctgatcg cctaacggtg cggtttgaaa tctcttctga tagttgaaat
     7141 cgccacaggt ttgacacatg gaatcgtaaa agaagtgaag cttcgtgtat tcggcattgc
     7201 atacgtagca attgcggggt gaactcagtt ctggcgcgga gtcatgagag ggcccttcta
     7261 tctgcgtggg agctttgaac accgtggctc tacgcgcgtc ccggatgcca gtggcggctc
     7321 tcgcggatct ttcctgtgcc actatggcag atcgttttgc ccgacgcaga gctttgtttc
     7381 tattcttttg ctccagcctg tttggtctgg cgatttgtcc tgccacacga atgagacgca
     7441 ctcttacttc ttcaggaagt tgtgtgaaat cgtctggatt tccggcccaa ctttccagag
     7501 tctctatgca actcagtagt tcctgatagc gattctcatc catatggatc gaaagcaggc
     7561 gctgccctgc ggttcaactg aaaaaaggaa cgattgtgct tgcctcatgg aagcgcggcc
     7621 tttgcatccg tagtcgtgac tgtggctgta agcagaaaca aactactggc gcgcattcgc
     7681 accgtattca tcccgcgcag gccggatatt cgttttttca gcgaagaatt gcattaccag
     7741 tccagccggg tggccgggtt cggtgcgttc ttgctcttgt tcgtgtgcct cccttacctg
     7801 tggctcgatc cgctcattca tcctggttct ccgtggatgc ttgttttgcg aattggccag
     7861 agtttgatgg gactgattgc acttgttgca gttttctttg atcgcgtgcg taagaatgcg
     7921 atgtacctcg tattcgtaaa tctactctac gtggaattat ccggagcagt gattgctggc
     7981 ctcaccggcg gagatccagt ttacaccgga ggattcgctc tggttcttat gattacatgc
     8041 ctggttcccg tgccacgcat gtatgctctc ttaacagcct ttgccagtgt tgccgtttac
     8101 gcctggttca tggtctctgc gaagctagat gtgtctatcc cgcgaaatat tttcgcgctg
     8161 cagctgatcg ttacctctct ttccattact gtgtttttta ttttcatcct ggatagaatt
     8221 cgccattcga gtttcgaaaa aactctagca atacaagcgg cgcgcgatga catcctgaag
     8281 ctgtctgagt ttgcacagaa ggcaaatcaa agcattgatt tgaacaaaat cctggatgaa
     8341 atcattgcat atgttcagga aaacttttcc attgaaggga cgcttcttca attgtacgat
     8401 cccaaaacag acgagctagt gtatttcaaa acaaacttcc aggattttca tgacgaagat
     8461 ctgcgaaagt atgtgcaggc gctccgtttt tctgccggaa agagaagctc ggccggagcc
     8521 atccttcgcc gcggaaaacc aatgtatttt cccaacatga aagttgcgcg aagaaatgaa
     8581 aaccaggacc ccctggacca cttcgtggtg cgcgctggaa tcgagtctgc tttgatcgtg
     8641 cctttgatcg ttgcggatgc acccgcggga atgctatatt tttctaacta tcatggagat
     8701 atgaatttat cccgggacca gctcagtact atttatgctt tctgtcagca gatcgggggc
     8761 gcgattcact ccagttcact gattcaccag gtggaacgtg aaaaagaaaa atcggaaagg
     8821 ctattgttga atattcttcc cgagagcgtg gctcttgagt tgaaggagaa cggtttcgtt
     8881 cgcccaacca tgttcgagaa cgtaaccatt ctgtttaccg atttcaaagg attcactcat
     8941 cacgcagcat ctatgtcacc tcaggatctg gtaacggaat tggatgggat ttttggacag
     9001 tttgatgcga tcgcagaaaa gtacggcctc gaaaagctca agaccatagg cgattcctac
     9061 atgtgcgtgg gcggccttcc aaaatcgaat cgaactcatc ccattgatgc gtgcctcgca
     9121 ggattagaaa tggcggcgtt tgtgaagcag gcaaatgaac ttcgcctggc cagtacag
//
LOCUS       DAOQJL010000089         9043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4099_length_9043_cov_4.438028, whole genome shotgun sequence.
ACCESSION   DAOQJL010000089 DAOQJL010000000
VERSION     DAOQJL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_9043_cov_4.438028"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLG78_05735"
     CDS             <1..673
                     /locus_tag="PLG78_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22645.1"
                     /translation="TRMFRAPLNSGSMGTAVLQNASEPSNSQFMTLDPTGRFLYGTIS
                     GTGGVNTFTTNGGASLTAQGNTLISGTEEPVRAQFLSDGSGMFLYTTSSGRLMSYSIN
                     ATTGMPIPVSNISVNVGALFIHPNQGYLYAGDTASGGLRLYRINGDKSLTFVNYYTGM
                     GGVSYDNGCFERGGQFLFLTKANATGVIQVVKINADLSPTLASSLTTGGNLGECTTSI
                     DSYWP"
     gene            799..871
                     /locus_tag="PLG78_05740"
     tRNA            799..871
                     /locus_tag="PLG78_05740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:832..834,aa:Thr,seq:ggt)
     gene            876..947
                     /locus_tag="PLG78_05745"
     tRNA            876..947
                     /locus_tag="PLG78_05745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:909..911,aa:Trp,seq:cca)
     gene            970..1155
                     /gene="secE"
                     /locus_tag="PLG78_05750"
     CDS             970..1155
                     /gene="secE"
                     /locus_tag="PLG78_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNE22646.1"
                     /translation="MLKFLKESREELRKVVWPSKEEVMNSTLVVLGAVVLISLFLFSI
                     DRMFSAMFDALIRLGSG"
     gene            1155..1688
                     /gene="nusG"
                     /locus_tag="PLG78_05755"
     CDS             1155..1688
                     /gene="nusG"
                     /locus_tag="PLG78_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNE22647.1"
                     /translation="MMSWYVIRTYSGHENKVKTNLEKLVQTRNLAGKVGQINIPTIKV
                     AEMKAGKKRVVEKKFMPGYILAELEMDDELQYTIRKLPSVAGFVGSPDPQALTADEVK
                     NLMSSEGAVEEEQPRARMLFSVGETVKIVDGPFANFNGVVDEIMPDKGRLRVKVEIFG
                     QATPVELDYLQVAANVR"
     gene            1715..2143
                     /gene="rplK"
                     /locus_tag="PLG78_05760"
     CDS             1715..2143
                     /gene="rplK"
                     /locus_tag="PLG78_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNE22648.1"
                     /translation="MAAKKIVTQVKLQVEAGKATPAPPVGPALGQHGLNIMEFCKQFN
                     ERTKNQVGIKIPVVITVFSDRSFTFITKSPPAALLIKKELGLESASATPNKAKVGTIK
                     RAQLEKIAEIKMKDLNCHDMDAAVKILAGTCRSMGVNVEG"
     gene            2145..2831
                     /gene="rplA"
                     /locus_tag="PLG78_05765"
     CDS             2145..2831
                     /gene="rplA"
                     /locus_tag="PLG78_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE22649.1"
                     /translation="MKRGKKYKAVVEKVERTRHYEPTEAAALMKEVSTSKFDGTVEAS
                     VKIGYKSLQNVRGIVRLPHGTGKEVRVLVFAKEDKQAEAKAAGADFVGGTEMVEKILK
                     EGWTDFDACVATPDLMKDVGKLGAILGRKGLMPKPKAGTVTTDVGTAVKNLKSGQMEY
                     RPDKTGVIHVRIGRVSFTPDKIEENMRAVFAAITRDRPSDAKGEYVRSFYVSPTMGPA
                     ARVNIRAMAT"
     gene            2843..3391
                     /gene="rplJ"
                     /locus_tag="PLG78_05770"
     CDS             2843..3391
                     /gene="rplJ"
                     /locus_tag="PLG78_05770"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNE22650.1"
                     /translation="MPAHATVISPENKESVTKLKEILSKHANFVVTTYSGLSVVDMGE
                     LRSAVREKDGRVKVVKNNLFRIALKESAVHASAADAISDKLKGPIAVTFAGADISAIS
                     KALVDFSKKNNKVEVITGFLEGKKLDKAEVQQIANLPSREQLLTIIGRGLNTPATKIA
                     IGMKEVITSLARGIRAVGEKNG"
     gene            3429..3812
                     /gene="rplL"
                     /locus_tag="PLG78_05775"
     CDS             3429..3812
                     /gene="rplL"
                     /locus_tag="PLG78_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNE22651.1"
                     /translation="MSTDALLESIGKLTLMEAAELVKKMEEKFGISTAAPVAVAAGPA
                     AGGAADAGAGEDATFNVVLKSHGEKKIDVIKVVREVTGLGLKEAKDLVEKAGSVVKEG
                     LAKAAAEDLKKKLEALGAQIELKAG"
     gene            3904..7578
                     /gene="rpoB"
                     /locus_tag="PLG78_05780"
     CDS             3904..7578
                     /gene="rpoB"
                     /locus_tag="PLG78_05780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNE22652.1"
                     /translation="MAQNKYVRLGTITDYDQIPDLIDIQFKSYDWFLQKDAGEAKRKS
                     QGLDAVFQETFPIESPNEDMVLEYVSYSLGDPRWDEEECKNRGTTYALPLKAIIRLIN
                     KLTGEVREQSVYMGDLPVMTEQGTFVINGAERVVVSQLHRSPGIFFFYDAEKRVYSAR
                     VIPYRGSWLELELDTKGILVARIDRKKKFPATLLLKSMGYGSDEDVLKLFYKIESLKL
                     AGLTGRDLKKIIGRRLAARVLNPENDEVIMYAGEKVNEDNLDILKEMKIKELKLLVFP
                     TSKDDVIVINCLEKEGTETQEAALQKFHTLMRPGEPFNPENAKADLNRLFFSEKSYDL
                     GGVGRYKINNKFAYHNPKEFTGIDTRALRPEDIVETMRYFVNLINEVPEYHFDDIDHL
                     GNRRVRSVGELLMNQLKVGFSRMERVVKERMTVQDIDVITPQALISIKPIMAVINEFF
                     GSSQLSQFMDQTNPLAELTHKRRLNALGPGGLSRERAGFEVRDIHYSHYGRMCPIETP
                     EGPNIGLIVSMSSYCRVNPYGFLETPYRKVASGKVSEKIEYLTADIEERYNIAQANAP
                     LEDNGHFKSKQISCRKRGDFPFCAPEEVQYMDMAPLQVVSVSTSLIPFLEHDDANRAL
                     MGSNMQRQAVPLLVDEAPFVGTGIEEKAAYDSRNCVVAKQDGMVLMVDSNKVVIRPTG
                     GKKEDVTYFLKKFKRSNQGTCVNQRPVVSVYAAPADGKVKITGDILEFTPAQGDPVQY
                     FLKTDDTEFHAVVKSGASVSKGDLLAGQAVKGEVRDSQGILTQKGTVLADGHSIDNGR
                     LALGKNVLVAFMPWEGYNFEDAILISERIVKDDVFTSIHIDEYEIQARETKLGQEIIT
                     RDIPNLSDKAFRDLDENGVIRIGAEVKAGDILVGMVTPKGESDLTPEYKLLHSIFGEK
                     AREVRDTSLRMPNGSEGVVIDIKKYSREAGDELAAGVEAMVKVFVAKKRKLQVGDKMA
                     GRHGNKGVVARVMPEEDMPFLKDGTIVDIVLNPLGVPSRMNIGQIFETMLGFAGKRLG
                     THFETPVFDGADEESIEKFLKQAELPTDCKFDIYDGRSGLPFEGKVFCGYIYMLKLAH
                     LVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGASHTLQELLTVKS
                     DDMLGRARIYEAIVKGVHSIRPGVPESFNVLMQELRGLALDVALYNQSDERVELSEIE
                     DELGRGGRRNKIKIETIEKS"
     gene            7592..>9043
                     /locus_tag="PLG78_05785"
     CDS             7592..>9043
                     /locus_tag="PLG78_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE22653.1"
                     /translation="MANVSVSDFESIRIQLASPDRIRDWSYGEVKKPETINYRTLRPE
                     RDGLFCERIFGTTKDWECYCGKFKSIRYKGVICDRCGVEVTHSKVRRERMGHVELATP
                     VAHIWYYRSVPSRMGLLLNLTINQLKSILYFEKYVIVDAGDSGRDKGELIDEEEYHEY
                     LDEYGDKFVAMIGGEAIKEMLTRIDVDAEIREIRAKIQEKGKVTDRRFLKRLEVLEAL
                     RESGNRPEWMMLEAIPVIPPELRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLAL
                     KAPEIIVRNERRMLQEAVDALFDNSRRKRSVKGKGNRPLKSLSDMLKGKTGRFRQNLL
                     GKRVDYSGRTVIVIGPDLKFHQMGLPKKMALELFKPFIMKRLVDLELAPNIKSAKKKV
                     ENEEKEVFEALEEVIKEHPVLLNRAPTLHRLGIQAFLPVLVEGKAIKLHPLVCHAFNA
                     DFDGDQMAIHVPLSPKAQLESWLLMLSPHNLLNP"
BASE COUNT         2466 a         2187 c         2325 g         2065 t
ORIGIN      
        1 cactcgcatg ttccgcgctc ctttgaattc gggaagcatg gggactgcgg ttttacagaa
       61 cgcaagcgag ccttccaaca gtcagttcat gactctggat ccaaccggta gattccttta
      121 cggcacgatc agcggaacgg gcggcgtaaa tacattcact acaaatggag gagcaagtct
      181 gactgctcag ggaaacactc tcatttccgg cacagaagaa ccggttcgcg ctcagttttt
      241 gtcagacggg tcgggcatgt tcctgtatac cacttcgagc ggccgattga tgtcttattc
      301 catcaatgca accaccggta tgcctattcc agtttccaac atatcagtca atgtgggtgc
      361 gcttttcatc catcctaacc agggatatct gtatgccggg gacactgcaa gcgggggact
      421 ccgcctctac agaatcaatg gggataaatc gctcactttc gtgaactact acaccgggat
      481 gggtggcgtg agttacgaca acgggtgctt tgagagagga ggccaattcc ttttccttac
      541 gaaggcaaat gcgactggtg taatccaggt tgtgaaaatc aatgcagacc tgagcccgac
      601 tctggcatcc agcctgacaa cgggaggcaa cctcggagaa tgcacaacgt ctattgactc
      661 ttactggccc tgaggcatct gcctttcgca ttcattcgcc ggcaggatga acgcaaattc
      721 cattccactt gacattagac gtacagataa attttatgga cattctcccg aacaggtatg
      781 tccggatcgg gggcgggtgc cctcttagct cagttggtag agcgcatcca tggtaaggat
      841 gaggtcacca gttcaagcct ggtagagggc tcaggaggcc ggtagttcaa tggctagaat
      901 aggggtctcc aaaacccttg atgagagttc gattctctcc cggcctgagg gaaccaggaa
      961 acgtagagta tgttgaagtt tctaaaagaa agtcgcgaag aactcaggaa ggttgtttgg
     1021 ccttccaaag aagaagttat gaattccact ctcgtcgtgc tcggcgctgt ggttttgatt
     1081 tctctgtttt tattttccat cgatcgtatg ttttcggcaa tgttcgatgc attgattcgg
     1141 ttggggtctg gctaatgatg agctggtacg tcatccggac ctactcgggc cacgagaaca
     1201 aggtcaagac caacctcgag aagctggtgc aaactcgcaa tctcgcaggc aaggtcggtc
     1261 agatcaacat ccccaccatc aaagtggcgg agatgaaggc tggcaagaag cgagtcgttg
     1321 aaaagaagtt catgccgggt tatattctgg ctgaactcga aatggacgat gagctgcagt
     1381 atactattag aaagcttcct tccgttgcgg gctttgtagg atcaccggat cctcaggcgc
     1441 ttacagcgga tgaagtgaag aatctgatga gttcagaggg agctgtggaa gaagagcaac
     1501 cgagagcaag aatgcttttc tcggtcggag aaaccgtcaa gatcgtggat ggtccgtttg
     1561 caaacttcaa cggtgtcgta gatgaaatca tgccggacaa aggccgcctt cgcgtgaagg
     1621 tggaaatctt tggccaggcc actcctgtag agctggatta tctgcaggtg gcggctaacg
     1681 ttcgatagca ttgtatagat tatagaggga ctagatggca gcgaagaaga tagtaacaca
     1741 ggtcaagttg caggtggaag caggaaaagc tacaccggcg cctccggttg gtccggctct
     1801 gggtcagcac ggcttgaaca ttatggaatt ctgcaagcag ttcaatgagc gcacaaagaa
     1861 tcaggtggga atcaagattc ctgttgtgat caccgtgttc tctgatcgca gctttacttt
     1921 catcaccaag tcgcctccgg ctgctcttct catcaagaag gagctgggtc ttgagtctgc
     1981 atctgcaact cccaacaagg cgaaagtcgg cacaatcaag cgtgcgcaac ttgagaagat
     2041 cgcagagatc aaaatgaagg acttgaattg ccatgacatg gacgcggcag tcaaaattct
     2101 ggcaggaaca tgccgatcca tgggcgtaaa cgtagagggt tgagatgaaa cgcggcaaga
     2161 aatacaaagc agttgtagaa aaagtcgaac gcactcgtca ttacgagcca acggaagcgg
     2221 cagcactcat gaaagaagtg agcacttcca aattcgacgg tacagtagaa gcatccgtta
     2281 aaatcggata caaatctctg cagaacgttc gcgggatcgt acgtcttccg catggcactg
     2341 gaaaagaagt tcgcgttctt gtgtttgcaa aagaagacaa gcaggcagaa gcaaaagcag
     2401 ccggtgctga ttttgttggc ggaacagaaa tggtagagaa gatcctgaaa gagggatgga
     2461 ccgatttcga cgcttgcgtt gctactcccg atctcatgaa ggatgtaggt aagctcggtg
     2521 cgatccttgg tcgcaagggc ctcatgccaa agccaaaagc tggaactgta accacagatg
     2581 ttgggacagc tgtgaagaat ctcaaatccg gccagatgga atatcgtccg gacaagactg
     2641 gtgtgattca tgttcgtatt ggccgcgtgt cttttacacc ggacaaaatt gaagaaaaca
     2701 tgcgcgcagt ttttgctgcc atcactcgtg atcgcccttc agacgcgaaa ggcgaatatg
     2761 ttagaagttt ctatgtgtct ccaactatgg gacccgctgc gcgtgtaaac atccgcgcta
     2821 tggcgactta aggaattaga aaatgccagc acatgcaact gtaatcagtc cagaaaacaa
     2881 agaaagcgta acaaagctca aagaaatctt gagcaagcat gccaacttcg ttgtgaccac
     2941 ttattcaggt ctcagcgttg tggacatggg cgagttgcgc tctgcagtgc gcgaaaagga
     3001 cggccgggta aaggtcgtga agaataacct ctttcgcatc gcactcaaag aatcagcggt
     3061 tcacgccagt gcggcagatg caatcagcga caagctcaaa gggccaatcg ctgtaacatt
     3121 tgcaggcgcg gacatttccg ctatatccaa agctctcgta gacttctcca agaagaacaa
     3181 caaggtggaa gtaatcaccg ggtttctcga agggaagaag ctcgataagg cggaagttca
     3241 gcagatcgcc aatcttcctt ctcgcgagca gctattgaca atcatcggac gcggcctcaa
     3301 cacgcctgcc acgaagattg caatcggcat gaaagaagtc atcacgagtc tcgctcgcgg
     3361 gatccgcgcc gtcggagaga aaaacggcta acgcaaaaat ttactgaaac cagggtaagg
     3421 agaaaacaat gtctacagac gcactgctcg aatcgatcgg gaaactgact cttatggagg
     3481 cagcagagct cgttaagaaa atggaggaga agttcggaat ctccaccgct gcaccagttg
     3541 cagtagctgc aggtccagcc gcaggcggag ccgctgacgc gggcgcaggc gaagatgcaa
     3601 cattcaacgt ggttctgaag agccatggtg agaagaaaat cgacgtgatc aaagtcgttc
     3661 gcgaagttac gggtcttggt ctgaaagaag ccaaagatct tgtagagaag gctggatccg
     3721 ttgttaaaga aggacttgct aaagcagcag ctgaagatct caagaagaag cttgaggcac
     3781 tcggcgctca aatcgagctc aaagcaggct aattgctcga ttccttttgt tcgcagcaca
     3841 gcagggcatc cgccctgctg attgcttttt tcttttgttt attttcctga ccaggagcag
     3901 cccgtggccc agaacaagta cgtacgtctc ggaacaatta cagactacga tcaaatcccg
     3961 gatttgatcg atatccagtt taaatcctac gactggtttc ttcaaaaaga cgctggagaa
     4021 gccaaacgga aaagtcaggg cctcgacgct gtttttcagg aaacattccc gattgagagc
     4081 cccaacgaag acatggtgct cgaatatgtt tcctattctc ttggcgatcc tcgctgggat
     4141 gaagaagaat gcaaaaatcg cggaaccaca tacgctcttc ctctcaaagc aatcattcgt
     4201 ttgatcaaca aactcacagg tgaagttcgt gagcagtctg tttatatggg agaccttcct
     4261 gtaatgacag agcagggcac gttcgtcatc aatggcgcag agcgcgtagt tgtatcacag
     4321 ctccatcgca gtcctggtat tttctttttc tacgacgcag agaagcgcgt gtattcagca
     4381 cgcgtcattc cttaccgtgg cagctggctg gaactggaac tcgacaccaa aggcattctg
     4441 gttgctcgca ttgaccgtaa gaaaaaattc ccggctacac tgcttctcaa gtctatgggc
     4501 tacggctcgg atgaagatgt actcaaactc ttctataaga ttgaaagcct gaagctcgcc
     4561 ggtctcacag ggcgcgatct caagaaaatc atcggcagac gccttgcagc gcgtgttctc
     4621 aatccagaga acgatgaagt gatcatgtat gcaggtgaga aagtcaatga ggacaacctc
     4681 gacattctca aagagatgaa gatcaaggaa ctcaagctcc ttgtattccc taccagcaaa
     4741 gacgacgtca ttgtcatcaa ctgtctggag aaagaaggaa cagagactca ggaagcggct
     4801 ctccagaagt tccacactct catgcgtccg ggtgaacctt tcaatccaga aaacgcaaaa
     4861 gcagatctca acagactctt cttctctgag aagtcgtatg atctaggcgg cgttggccgt
     4921 tacaagatca acaacaagtt cgcataccac aacccgaaag aattcactgg aatcgacaca
     4981 cgtgctcttc gtccggaaga catcgtagaa accatgcgct acttcgtgaa tttgatcaac
     5041 gaagtacctg aatatcactt cgatgacatt gatcaccttg gaaatcgccg cgtaagaagc
     5101 gtgggcgaac ttttgatgaa ccagttgaaa gttggtttca gcagaatgga acgcgttgtc
     5161 aaagagcgca tgacggttca agacattgac gtcatcactc cgcaggctct gatttccatt
     5221 aaaccaatca tggctgtcat caatgagttc tttggttcca gccagctttc acagttcatg
     5281 gaccagacga atcctctcgc agagctcact cacaagcgtc gtctcaacgc actcggtcct
     5341 ggtggtcttt ctcgcgagcg tgcaggcttc gaagttcgtg acattcacta cagccactat
     5401 ggccgtatgt gtccgattga aactccggaa ggtccaaaca ttggtctgat cgtttcgatg
     5461 agttcttatt gccgtgtgaa tccatacgga ttcctggaaa ctccttatcg caaggttgct
     5521 tccggcaaag tttcagaaaa gatcgaatat ctcacggcag acattgaaga gcgttacaac
     5581 attgctcagg caaacgctcc tctagaagac aacggtcatt tcaagtccaa gcagatcagc
     5641 tgccgcaagc gcggcgattt cccattctgt gctccagaag aagtgcagta catggacatg
     5701 gctcctctgc aggtagtgag cgtttctact tctctcattc cattcctcga gcacgatgat
     5761 gcgaaccgcg cactcatggg ctcaaacatg cagcgtcagg ctgtgcctct tcttgtagat
     5821 gaagctcctt ttgttggaac aggcatcgaa gaaaaagcag cctacgattc gcgcaactgt
     5881 gtggtcgcaa agcaggacgg catggtgctg atggtggatt ccaacaaagt tgtgattcgt
     5941 ccaacaggcg ggaaaaaaga agacgtaacg tattttctca agaaattcaa aagatccaac
     6001 cagggaacct gtgtgaacca gcgtccagtg gtcagcgtgt atgctgcacc tgcagacggt
     6061 aaggtgaaaa tcacaggaga cattcttgaa ttcactccag ctcagggaga tccagttcag
     6121 tatttcctga aaacagatga cactgaattc cacgctgtag tgaaaagcgg cgcttctgtt
     6181 tccaagggag atctcctcgc cggccaggca gtcaaagggg aagttcgcga ttcccaggga
     6241 attctcactc aaaagggaac tgttcttgcg gacggtcatt ccatcgacaa cggaaggctg
     6301 gcacttggta agaatgtgct cgttgcattc atgccatggg aaggttacaa cttcgaagat
     6361 gcgatcctca tcagcgaacg cattgttaaa gatgacgtat tcacctctat tcacattgat
     6421 gaatatgaaa tccaggcgcg cgaaacaaag ctcggtcagg aaatcattac acgtgacatt
     6481 ccaaacctca gcgacaaggc attccgtgat ctcgacgaaa acggtgtgat ccgcattggt
     6541 gcagaagtaa aagccggaga cattctcgtt ggtatggtta cgcctaaagg cgaatcagat
     6601 ctgactcctg aatacaaact actgcacagc atcttcggtg aaaaagctcg cgaagtgcgc
     6661 gacacatccc ttcgcatgcc aaacggatcc gaaggcgtgg tcatcgacat caagaagtac
     6721 tctcgcgaag caggggatga acttgcagcg ggcgtggaag caatggtcaa agtattcgtt
     6781 gcaaagaagc gcaagcttca ggtgggcgat aaaatggccg gccgccatgg aaacaaaggt
     6841 gttgttgcgc gcgtgatgcc ggaagaagat atgccgttcc tgaaagacgg aaccattgta
     6901 gatatcgttc tcaatccgct tggtgttcca tctcgtatga acatcggtca gattttcgaa
     6961 accatgcttg gcttcgcagg caagagactt ggtactcact ttgaaacgcc ggtattcgac
     7021 ggcgcggacg aagagagcat tgagaaattc ctcaagcaag cggaattgcc tacagactgc
     7081 aagtttgaca tttacgatgg ccgctccggt cttcctttcg aaggaaaggt tttctgcgga
     7141 tacatctaca tgttgaagct cgctcacctc gtagacgaca agatccatgc tcgttctact
     7201 ggaccatact cacttgtgac tcagcagcca ctgggcggga aagctcagtt cggcggccag
     7261 agactcgggg aaatggaagt gtgggcactc gaagcatacg gcgcttcgca tactttgcag
     7321 gaacttctga ctgtgaagtc agatgatatg cttggccgcg ctcgtatcta cgaagcgatt
     7381 gtgaaaggtg ttcacagcat tcgtcctggt gttccagaat cgtttaacgt tcttatgcaa
     7441 gaactcagag gcctggctct tgacgtcgct ctctataacc agagcgatga aagagtagaa
     7501 ttgtccgaaa tcgaagacga actcggacgc ggcggccgcc gcaacaagat caagatcgag
     7561 accatcgaga agagctaaga ggcaataaga aatggcaaat gtaagcgtca gcgactttga
     7621 atcaattcgc atccaacttg catctccgga tcgcatccgg gactggtctt acggcgaagt
     7681 aaaaaagcca gagactatca actaccgtac actccgcccg gagcgcgatg gtctgttctg
     7741 cgagcgaatt ttcggaacta caaaagactg ggaatgctac tgcggtaagt tcaagtccat
     7801 ccgctacaag ggtgtgattt gcgatcgttg cggtgtggaa gtaactcaca gcaaagtgcg
     7861 tcgcgaacgc atgggtcatg tggaactcgc cactcctgtg gctcacatct ggtactatcg
     7921 ctctgtgcct tctcgcatgg gtcttcttct gaacctgacc atcaaccagc tcaagagcat
     7981 tctctatttc gaaaagtacg tcatcgtgga tgccggggat tcaggccgcg acaaaggcga
     8041 actcatcgat gaagaagagt atcatgagta cctggacgaa tacggcgaca agttcgtagc
     8101 gatgatcggc ggagaagcaa tcaaagaaat gctcacccgc attgatgtgg acgcagagat
     8161 ccgcgaaatc cgtgcgaaga tccaggagaa gggcaaggtc acagaccgcc gcttcctcaa
     8221 gagactcgaa gtactcgaag cactccgcga atcgggcaac cgtcctgagt ggatgatgct
     8281 cgaagcaatt ccagtcatcc cgccagaact tcgtcccatg gttcagctcg aaggcggtcg
     8341 tttcgcaacg tctgacctca acgatcttta tcgccgcgtt atcaatagaa acaatcgtct
     8401 caaaagactt ctggctctga aagctcccga gatcatcgtt cgaaacgaac gtcgcatgct
     8461 gcaagaagca gtggacgcat tgtttgataa cagccgtcgc aagcgttccg taaaaggaaa
     8521 agggaatcgt cctttgaaat cactttcaga tatgctgaaa ggaaagacag gccgtttccg
     8581 tcagaacctt ctgggcaaac gcgtagacta ctccggtaga actgtaatcg taattggacc
     8641 agatcttaaa ttccatcaga tgggccttcc caagaagatg gcactcgaac tcttcaagcc
     8701 cttcatcatg aagcgtcttg tggacctgga acttgcacct aacatcaagt ctgcgaagaa
     8761 gaaagtagag aacgaagaga aagaagtttt cgaagctctc gaagaagtga tcaaggaaca
     8821 tccggttctc ttgaaccgcg ctcctactct tcatcgcctt gggattcagg cattccttcc
     8881 agttcttgta gaaggaaaag caatcaaact tcatcctctt gtttgccatg cattcaacgc
     8941 ggactttgac ggtgaccaga tggcgattca cgtccctctg tctcctaaag cgcagcttga
     9001 atcctggctc ttgatgcttt ctccgcacaa tctgctgaac ccg
//
LOCUS       DAOQJL010000090         8933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4171_length_8933_cov_4.565330, whole genome shotgun sequence.
ACCESSION   DAOQJL010000090 DAOQJL010000000
VERSION     DAOQJL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_8933_cov_4.565330"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="PLG78_05790"
     CDS             <1..1872
                     /locus_tag="PLG78_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22654.1"
                     /translation="IAVELPADLSGALDQVLPSADQIPVISFDDAGAQSVHFILEPLE
                     PIVEAVRSAKDRGIHTHLIDLPCLSASPADAEFFSPASYGFSPEQFPDTFSLRVMSAR
                     TVYDLYRKNQLPVRYESLDPSSLRGDFIRELFMAEKIRKLAPLYSTPRTGRIDGCLLV
                     VCGIKHLPAIEHLVALPEHEFERFRGILGEIQRYRGQEEPEDDEEPLEALMKGSEARN
                     SAPDVEISVLSRESPEVLSQPGYYNTTWNLVRRSERTVKAFNRIVLQRAVYRESVSRF
                     EKESGEALSPRIEKNFVRFARTWSWLENKLLPDAYRLVMSARAFGGDNFARIFYDILN
                     FLPPLRSPAYPEKKLTLDDIYRDSKMIRFRLRLKKKKRVPPPPVRKKFQREKYPGEWR
                     DSFKGGGICSYPPEDIVIEDFGRYLQKRAVSVLQGSDSKTLPFTASLLDGIDYRETIR
                     NLHMGKVFVRDLRNRGIEAGSVVLIFSEDDIEHDWKVVWWGEHNQESDMALYATSPGN
                     EIIGPGICRCRYGGLMMTYPPRRLHDIWEDPNYNEFEKPSDRLLAAAIDYNEKNAVVH
                     LAHRPPSPKLVAIANRLGQKVIHIPISTMNSVLLGRIRRFHVLDSHDRRGDAGGFIW"
     gene            complement(1922..5782)
                     /locus_tag="PLG78_05795"
     CDS             complement(1922..5782)
                     /locus_tag="PLG78_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22655.1"
                     /translation="MDDPGRLTLAERYRQGIESLIHFDFERREEDLKWMLAESKKPVK
                     GISWLKEKYYELLRKCEEHPVPFQLIYDLETIASRLFPRAARKFSFIKDPIPEYYRAR
                     LKIYEPELREIADSEGHIRGADLLGVLGKMGARDGRSMVEMFLARLKDGKSHSSLASK
                     LEPGALEQLEVSLQRIPPNVPPEDLPALMQVAAQSSVSPIENEKLESHEAIDELGESE
                     TPAVELPLAMEVGMEDSGGWKEDSASEIADVQTDDFAEAFSETSALKEDESQHNADIP
                     GEVDVRDDSVLDFASTSAEPFHSDSILATGAEALESPSTDYAADTDSTFSNLEVSEET
                     LASEDSLRDHLARPDHRTDLDLKSDAAYKQFLIEQSELTQLRKERFLELSGLPGRIDQ
                     AIEQRNESESVQLLAEIHPLAFLTEQERLLRGLDAIESPLGKIMGHLSEMYRRLNTPD
                     MREYVDYYSAKLEAGGLFDTFPTIRERLINYRNSLVDPPPELLKQVHTGLRDIEDRTG
                     EDHRECVAQQVRFLHECLENEVFGKVWPTIAVILENILKEELASLGETDAPIVSWRNR
                     LQREEQTTPRAIANILAEVEPESGSAIAGILSTTDLPSATARPPASGLSSATPGHRGT
                     GFDFLANRSLDPHGDAQKMQESLTRQFATDAHAAHALSIQILNSSEYSLLEKETTLSV
                     LERANLAAATELYHFDRLVSSAGTLDDQLAFAASLRNGSAGHLVADRLQSLEFRRAQN
                     QSMGHFSAFLATKDPSAKTRLFQEIARDPHIPEGMKSVFRKGADANSFEKFLESITTS
                     SQSNANKLKLLRILQKIAVHRNWLTPSTKERLENRIRFYERILSADQEIAASVVGRLD
                     YYRTRREEIRQALMTGKPAREKLTPPNVESPAVESAEEVHRHGFSDELLESLIQSPMR
                     ASRWYSKLPESSKEELHKQATSPGKAQILVNQIQKDIAFKKAIRHLKKPVEAAVFSEL
                     SAQFPEPEYQEKLRQFTPSQKETKVVVAQSQSASEDSSTNIPSGAIGPDASFDQLVNQ
                     TLLSGPDLPPLPEEDLALDHRPGPPHPAFAAAISKNEERTKHDVTSSKPTPPHPQHHA
                     EPSHHEGTRHEPHHERHPEKEHPSHAARAKTESAVDGLAASLARGFSGLFSKKTSAPS
                     HGKEQKAQSSSHGAHDKKSGKKKTDQAAATLMKAVSSYAKGEIYLSEKDLKSLEKEME
                     KHPNAKRLIEDHAYLFKVEHPDPAQFQPPIFVIIPEMKALNHDPTKKKAVMTRIEQSI
                     SSAPSKKKAHMQALLTFMERYKTK"
     gene            complement(5888..6889)
                     /locus_tag="PLG78_05800"
     CDS             complement(5888..6889)
                     /locus_tag="PLG78_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE22656.1"
                     /translation="MIEWLALIQESRRNRFLFFFLCLVIETGVALIHTSLPYYANLIL
                     LYAFPIIGASWILGARYGLTLALFSTVIWWVTQTSNRPEEVAETVHIWNACSRAIIFF
                     GIVLLISFLKEALTARERTLARLLARRSFLASVSHEMREPLKRILAGSEILSTTASDP
                     AAVIAVQAATQELMELVDAILELARLESGAVSFRADFNPVIVLREVVEHLTPVARQRN
                     QMFSVEIDGNVPPVVRADQNCLRYSITRLLRDASEFSESGAIIINLGYSREDQLLRFT
                     VRDSGQANADQRNTSVRDGLGMHVAREMARLNGGHLWQDENKTSEAIHHFTMIVDAV"
     gene            7186..7923
                     /locus_tag="PLG78_05805"
     CDS             7186..7923
                     /locus_tag="PLG78_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE22657.1"
                     /translation="MIPFYRILLVEDDPGFAESVMRVFAAFNILLDSATDGRVALIWV
                     RSRPYDAVITDLRMPIVGGLAFIESATAFLTGKPVLVMTASNQREDVLSARELKVDDY
                     ITKPVKPSELVLRLARKLPATPGLVPLQRFPLQIHYKSLGSAGVSISLEGVPGPDFEK
                     AFGDMLADLRNHLGTDLASVEFQVASEFGYDPRSIYALEKALDLLSRRLRVPGPRIHL
                     SGGYWDDARETHGDEARRGILGSVRWP"
     gene            7914..>8933
                     /locus_tag="PLG78_05810"
     CDS             7914..>8933
                     /locus_tag="PLG78_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE22658.1"
                     /translation="MALRYIAPFVLAAVIFSLDLMVPRGFAVWLWYIPALFVSRPHGK
                     LLAIEVWIFSVLMLGGAMMSPESSVPVAVVFANRSLGIALMAVVALLLYTGGRMNEAL
                     RLAHQRAEEASEAKTRFLGYVSHDMRTPLNAIIARLDVIKADAQTAHVLRDLNYIESH
                     ASFLSAFIHELLDYANGNENEIEIAPVRLREVFDSIAALFQPLIPEGVSLDISVSSEL
                     EFPTDKRRLQHILLNLIANSVKFTQFGSISVYAAESPAGLHLTIDDTGSGVSQAMLEK
                     MFEHGITGDPDGLGLGLAMTKYFVRFLGGYVSAFSDGPGAGLSVEILIPHPDRRTHHF
                     TTRGNP"
BASE COUNT         1972 a         2214 c         2333 g         2414 t
ORIGIN      
        1 attgccgttg agttgcccgc ggatctgtcc ggagccctgg atcaagtatt gccgagcgca
       61 gatcaaattc cagtgatctc ttttgacgac gctggcgcgc aatcagtgca cttcattctg
      121 gaaccacttg agccaatcgt ggaagctgtg cgaagtgcga aggatagggg aattcatact
      181 catctgatcg atcttccttg tctgtccgca agccccgcgg acgcagaatt cttttctcct
      241 gcgagctacg gtttttctcc cgaacagttt ccggatacgt tttcgcttcg ggtgatgagt
      301 gctcgcacag tatatgatct ttatagaaaa aatcagctcc cggtgcgata cgagtctctc
      361 gatccttctt ctttgcgagg agactttatt cgcgagcttt tcatggcgga gaaaattcgc
      421 aaactcgctc cactttattc tactccacgc accggacgca tagatggttg cctcctggtt
      481 gtgtgtggaa tcaagcatct gcctgccatt gagcacctcg tcgccctgcc ggagcatgag
      541 ttcgaacgct tccgcggaat tcttggagag attcaaagat accgcggcca ggaagaaccc
      601 gaagacgatg aagaacctct cgaagcattg atgaaaggat cggaagcgcg taactctgct
      661 cccgatgttg agatcagcgt gctttcgcgc gaatcgcccg aagttctttc tcagcctggt
      721 tactacaata ctacgtggaa tctggttcgc agatcagaga ggacggtcaa ggcgttcaat
      781 cgaattgtac tccagcgtgc cgtgtatcgt gagtctgtaa gcagatttga aaaggaaagt
      841 ggagaggcac tctccccaag aattgaaaag aattttgtgc gttttgcgcg aacctggagc
      901 tggctggaaa ataagcttct gccagatgct tacaggctgg tgatgtctgc gagagctttt
      961 ggcggagaca actttgctcg tatcttttat gacattctga actttcttcc tcctcttcgc
     1021 agtccggctt atcctgagaa aaaattaacg ttagacgaca tctatcgcga tagtaaaatg
     1081 attcgatttc gacttcgact caagaagaag aagcgagtcc ctccacctcc agttcgcaag
     1141 aaatttcagc gcgagaagta tccgggtgaa tggcgagatt ctttcaaggg tggcggaatt
     1201 tgctcttacc cgccggaaga cattgtgatt gaggactttg gccgctattt acagaaacgc
     1261 gctgtctctg tcttgcaggg ttctgattca aagactctgc cgtttaccgc cagtttgctc
     1321 gatggaattg attatcgcga aacaatccgc aacttgcata tgggtaaagt atttgtgcgc
     1381 gatctgcgca atcgaggaat tgaggccggc agtgtggttt tgattttcag cgaagatgac
     1441 atcgagcacg attggaaggt ggtctggtgg ggagaacaca atcaggaaag cgacatggcg
     1501 ctctatgcta cttctcctgg aaatgagatc atcgggcctg gcatttgccg ctgtcgctac
     1561 ggcgggctca tgatgaccta tccaccaagg agactccatg acatctggga agatcccaat
     1621 tacaatgaat tcgaaaaacc atcggatcgt cttcttgcgg cggccattga ctacaatgag
     1681 aagaatgcag tggttcatct agcgcatcgg ccaccttctc cgaagttagt cgccattgca
     1741 aatcgtctgg gtcagaaagt gattcatatt ccgatttcta caatgaatag cgtgctgctt
     1801 ggacgcattc gcagatttca tgtacttgat tcgcacgata gaagagggga tgcgggcggc
     1861 ttcatctggt aaggtgaacg cctggccttt ctggcagcgc tcgccactgg ttctcccaac
     1921 ttcacttcgt cttatatctt tccataaacg tgagtaacgc ctgcatgtga gccttctttt
     1981 tggaaggcgc gctgctgatc gactgttcaa tcctggtcat cacagctttc ttcttagtag
     2041 gatcatggtt caacgccttc atttccggaa tgatgacgaa tataggaggc tgaaattgag
     2101 ccgggtctgg gtgctccacc ttaaataagt aagcgtgatc ttcgatcagc ctttttgcat
     2161 tcggatgttt ttccatttcc ttctcaagag atttcaaatc cttttcgctc aagtatatct
     2221 ctcccttcgc gtacgatgaa actgccttca tgagagtagc ggccgcttga tctgttttct
     2281 tcttccctga tttcttgtca tgcgcgccat gggaagaaga ctgcgccttc tgctcttttc
     2341 catgcgaggg cgcgcttgtt ttctttgaga acaggccgct gaaaccgcgc gcgagagacg
     2401 cagccaggcc gtccactgca ctttctgtct tcgcgcgagc cgcatgtgaa ggatgctcct
     2461 tttctggatg acgttcgtga tgtggttcgt gacgcgtgcc ttcatgatgc gaaggctctg
     2521 catgatgctg tggatgaggt ggagtgggct ttgaagatgt aacgtcgtgc tttgtgcgtt
     2581 cctcgttctt cgaaatggcc gccgcgaagg ccggatgcgg tggtcccggg cgatggtcca
     2641 gggccaggtc ctcttccgga agaggtggaa gatctggacc ggatagcagg gtctggttta
     2701 ccagttgatc aaaactagca tctggaccaa tggcgcctga cggtatattt gtagatgaat
     2761 cttcgcttgc cgattgcgac tgcgcgacca ctactttcgt ttccttttga gacggcgtaa
     2821 attgcctcag cttctcctgg tattctggct ccggaaactg ggcggaaagc tcagaaaaca
     2881 cggcggcttc taccggtttt ttcaaatgac gtattgcctt tttgaatgct atgtccttct
     2941 ggatctggtt gacaaggatc tgagctttac ccggggaagt ggcctgtttg tgtaactcct
     3001 cttttgagct ttccggcagt ttcgaatacc acctggatgc gcgcattgga ctctggatta
     3061 gactctccag caattcgtca ctgaatccat gccggtgcac ttcttctgca gattctactg
     3121 caggagattc tacgttaggc ggcgttagtt tctctcttgc aggcttcccg gtcatgaggg
     3181 cttgtctgat ttcctctctg cgcgttctgt agtagtccag ccgaccaact acactggcgg
     3241 cgatctcttg atccgcgctt aagattcgtt cgtaaaatcg aatcctgttt tccagtcttt
     3301 cttttgttga tggagtcagc cagttcctat ggactgcgat tttttgcaga attctcagta
     3361 gtttcagttt gtttgcgttg gattggcttg aggtggtgat actctccagg aacttctcga
     3421 aagagtttgc gtctgctccc ttgcgaaaga cagacttcat cccttccgga atgtgcgggt
     3481 cacgcgcaat ttcctgaaag agacgggtct tggctgacgg gtctttggtt gcaagaaaag
     3541 cagaaaaatg gcccatcgat tgattttgag cgcgacgaaa ttcaagggac tgaagtctat
     3601 ccgcaacaag gtgaccggcg ctgccgtttc tcaaagatgc agcgaatgcc agttggtcgt
     3661 ctaacgtccc ggcagaagat acgagtctgt caaaatgata tagctccgtg gctgcagcta
     3721 gattcgccct ttcgagtacg gaaagggtgg tttctttttc aagaaggctg tattcgctcg
     3781 aattgagaat ctgaatcgaa agagcatgag cagcgtgcgc gtctgtcgcg aattgtctgg
     3841 tcagtgactc ctgcattttc tgtgcatctc catgcgggtc aagagagcga tttgcaagaa
     3901 aatcaaaacc tgtgcctcga tgacccggcg ttgcagatga aaggccgctc gccgggggac
     3961 gggccgttgc agaaggaagg tccgtcgtag aaaggatacc tgcaatggca gatccactct
     4021 ccggttccac ttccgcgaga atatttgcga tggccctggg agtggtttgt tcttctcttt
     4081 gaagtctgtt tcgccacgaa acgataggag cgtctgtctc tccgagcgag gcgagctctt
     4141 cttttagaat attttccagg atcactgcaa tggtgggcca gacttttccg aagacttcgt
     4201 tctccagaca ttcatgtaga aatcgcactt gctgagccac gcattcgcga tgatcttcgc
     4261 cggttctgtc ctcgatgtcg cgcagaccag tgtggacttg cttgagtagt tctggcggag
     4321 gatctaccag gctgtttcta tagttgatca atctttcgcg gatggttgga aacgtgtcga
     4381 agagtccccc ggcctcaagc tttgctgaat agtaatccac atactcccgc atgtccggag
     4441 tattgagtct acgatacatc tctgaaagat gtcccatgat ttttccaagt gggctttcaa
     4501 tcgcatcgag accgcgaagc aaccgttctt gttccgtgag aaatgcaagc ggatgaatct
     4561 ccgcgagcaa ttgaacggat tccgattcat tgcgctgctc gatggcttga tcgattcgcc
     4621 ccggaagtcc ggacagttcc agaaaccttt ctttgcgaag ctgcgtgagc tcggattgct
     4681 cgatgagaaa ctgtttgtat gcagcgtccg atttcagatc gagatcggtg cgatgatcgg
     4741 ggcgagcgag atgatcgcgg agtgaatctt cggaggcgag tgtttcttct gacacttcca
     4801 gattggagaa agtagaatct gtgtctgccg cgtaatctgt gctcgggctt tccagtgcct
     4861 ctgcgcctgt tgcgagaatt gaatcggaat gaaaaggctc agccgaagtg gatgcaaaat
     4921 ccagcacaga gtcatcccgc acatcgactt cgcctggaat atctgcattg tgttggctct
     4981 cgtcttcttt gagtgcgctt gtttcggaaa acgcctctgc gaaatcgtct gtctggacat
     5041 ctgcgatttc tgacgccgaa tcttctttcc atccgccgga atcctccatg cccacttcca
     5101 tcgccagggg aagctcgacc gctggtgtct cactttcgcc caattcatcg attgcttcgt
     5161 gcgattcgag cttctcattt tcgataggag acactgaaga ctgggccgcc acctgcatga
     5221 gtgccggaag atcttccgga ggaacgttag gcggaattct ctgcagcgag acctcaagct
     5281 gttcgagagc tcctggttcc agtttgcttg cgagagatga atggctcttc ccatctttca
     5341 atctggccaa aaacatttct accatactcc ggccgtctct ggcccccatt tttccgagca
     5401 ctccaagcag gtctgcgccg cggatgtgtc cttctgagtc tgcgatctct ctgagttcag
     5461 gttcgtagat cttcaagcgt gccctgtagt attccgggat tggatctttg ataaatgaaa
     5521 attttcttgc ggctctggga aacaggcgtg atgcaatcgt ttcgagatca tagatgagtt
     5581 gaaagggaac tggatgttcc tcacactttc gcagtagctc gtaatacttt tccttgagcc
     5641 acgaaatgcc tttgactggc ttcttggatt ctgcaagcat ccattttaga tcctcttcgc
     5701 gtctttcgaa atcgaagtgg atgagcgatt cgatgccctg gcggtaacgt tctgcgagtg
     5761 taagtctgcc tggatcgtcc attgcgtacg gtcttcagca agtcgtgggc ttcctcgcgc
     5821 gcgcaacaaa aaagcggcga tttccagtgg aatatgcccc cagccggata tgattcatgt
     5881 catgcaatca tacggcgtcc acaatcattg tgaaatgatg aatcgcttcg gatgtcttat
     5941 tttcgtcctg ccagagatgt ccgccgttga gacgggccat ttcgcgtgcc acatgcattc
     6001 ccagaccatc tcgaacagag gtattcctct gatctgcgtt tgcttgaccc gaatctcgaa
     6061 ccgtgaaacg cagcaactga tcttccctgg agtagccgag gttgatgatg atggcacccg
     6121 attcggaaaa ctcagacgcg tcgcgtaaga gcctggttat ggaatacctg aggcagttct
     6181 gatcagctcg caccactggc ggcacgtttc cgtctatctc tacagaaaac atttgatttc
     6241 tctgccgggc aacgggtgtc agatgctcta ctacttcgcg aagaacaatt actggattaa
     6301 agtctgcgcg aaatgacact gcaccgctct caagtctagc gagttctaga atcgcatcca
     6361 ccagctccat cagttcttgc gtggctgctt gcacagcgat aactgctgcc gggtcgcttg
     6421 cagtggtcga aagtatttca cttccagcga ggattcgttt caatggctcg cgcatctcat
     6481 gagagaccga tgcaagaaac gatcttcgcg caagtaggcg cgccagtgtt cgctcccggg
     6541 ccgtcagtgc ttcctttaga aatgaaatca gcagaacaat gccgaagaag atgatcgcgc
     6601 gagagcatgc gttccagatg tggacagtct ctgctacctc ttccgggcga ttgctggtct
     6661 gtgtcaccca ccagatcacc gttgagaaaa gagcgagcgt tagaccgtat cgcgcgccaa
     6721 gaatccacga agcgccgatg attggaaaag cgtagagaag gatgagattg gcgtagtaag
     6781 gtagggacgt gtgaatcaat gcaactccgg tttctataac gagacaaaga aaaaagaata
     6841 agaaacgatt cctgcgagat tcctggatga gcgccaacca ttctatcata ccacaatcat
     6901 caccgattgc gtggatgttg caagcggaaa gggaagatat ttccgaatca cgcatctgac
     6961 cttcgcagta ggtcctgctc ttttttgttc ggaattgtga atccggaaag tatttgatcg
     7021 aaattcggca gctatcgtct ataaagtcaa ttcgaatctc atttcattga gcgcataacg
     7081 ttattttttc ccaggcaaag acattctaca ttgcatgttc ctccgttcgc tagatttttg
     7141 acgtcggagg atgggtcgat ccaaaggttt cggctcaaaa ggaagatgat tcccttctat
     7201 cgaatccttc tagtagagga tgacccgggc ttcgctgaat cggtaatgcg cgtgtttgca
     7261 gcgtttaaca ttctgctcga ttcggcgaca gacggacgag tggctttgat ctgggtacgg
     7321 tcccgcccct atgatgccgt tatcaccgat ctacggatgc caattgttgg cggattggcc
     7381 ttcatagaat ccgccacagc gtttctaact gggaagcctg tgcttgtaat gaccgcttca
     7441 aatcaacgtg aggatgttct gtccgctcgt gaactgaaag tagacgacta tattaccaaa
     7501 ccggtgaagc catctgagct ggtcctgcgc cttgctcgaa aattaccggc aacgccggga
     7561 cttgtacctc tccagcgctt tcctttgcag attcattaca agagtctcgg atcggcagga
     7621 gtgagtattt cgttagaggg cgtaccggga cctgattttg aaaaagcctt cggtgacatg
     7681 ctggcggatt tgcgaaatca tcttgggacg gatctggcgt cggttgagtt tcaggtggca
     7741 agcgagttcg gctatgatcc acgttctatc tatgctctag agaaagcgct ggatctttta
     7801 tcgcggagac tacgtgttcc gggcccccgg attcatcttt ccggcggata ttgggacgat
     7861 gcacgggaaa cacacggcga cgaagcccgc agaggaatac tggggagcgt tcggtggccc
     7921 tgagatacat cgcgcctttt gttctggcag ctgtcatttt ttccctggac cttatggttc
     7981 cgcgaggatt cgcagtatgg ctctggtata tcccagccct cttcgtttcg cgaccacatg
     8041 gcaagttact ggccatcgaa gtctggatat tctcagtgct gatgcttggc ggagcgatga
     8101 tgtctccgga atcaagcgtt cccgttgcag tggtctttgc gaatcgttcc ctgggcattg
     8161 ctttgatggc agttgtggct cttttgctct acacaggcgg caggatgaat gaagctcttc
     8221 gcctcgcaca ccagcgagcc gaggaggcaa gcgaggcgaa aacgcgcttt ctcggttatg
     8281 tgagtcatga tatgcgaacg cccttaaacg cgatcatcgc tcgcctcgat gtcatcaaag
     8341 cggatgcaca aacagcgcac gtcttgcgtg acttgaatta tattgaaagc catgcttcat
     8401 tcttgagtgc cttcattcat gagctcctcg actacgcgaa cggaaatgag aacgaaatcg
     8461 aaatcgcgcc ggtacgtctc cgtgaggtat ttgactctat tgctgccctt tttcagcccc
     8521 tcattcccga aggggtttcg ctggacatca gtgtttcttc ggagctggag ttcccgacgg
     8581 ataagcgcag attgcagcac atcttgctca acctcattgc aaactctgtc aagttcacgc
     8641 aatttggatc aatatccgtt tacgcagcag agagccctgc cggtcttcac ttgacgatcg
     8701 atgataccgg cagcggtgtt tcgcaagcca tgctggagaa aatgtttgaa catggcataa
     8761 ccggcgatcc tgacggactg ggccttggac ttgcgatgac aaagtacttt gttcgttttc
     8821 tcggcggata cgtgagcgcc ttttcggatg gtcccggggc tggtctgtcc gttgaaatcc
     8881 taataccgca tccggacagg cgcactcacc atttcacgac acgaggcaat cct
//
LOCUS       DAOQJL010000091         8911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4186_length_8911_cov_4.666667, whole genome shotgun sequence.
ACCESSION   DAOQJL010000091 DAOQJL010000000
VERSION     DAOQJL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4186_length_8911_cov_4.666667"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLG78_05815"
     CDS             <1..382
                     /locus_tag="PLG78_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE22659.1"
                     /translation="GENVEPVPIENKLVESQYIDQCMVVGQDQKFLAVLVVPSLDGFA
                     RGGLKAKTLSELASNEEAGKVITAEIKRIVSADQGFKSFERVQDFRFITKPFEVGDEM
                     TNLFKLKRHVITEKYKKEINSMYK"
     gene            443..919
                     /locus_tag="PLG78_05820"
     CDS             443..919
                     /locus_tag="PLG78_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22660.1"
                     /translation="MFGLTQLGIVHTLIALIAVGTGAFLLIREGRISPAGLAGKIYVF
                     ATIVACITALAIYQHGGFGKGHVLAILTLVVLGVATAAGRGMFGGVSKAVETVSYSAT
                     FLFHWIPGVSEATTRLPLGSPLMNGPEDPALKTILGVLFVLFVVGSVLQVRSLRKS"
     gene            984..4103
                     /locus_tag="PLG78_05825"
     CDS             984..4103
                     /locus_tag="PLG78_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22661.1"
                     /translation="MSGNLFLNFFSIGALIPLLFVMFIALLLIRIRDKGESTWWLASF
                     AIVYCLFMLPYFPASVLFHPAGAYHRWFTVPFVLLTFTFLAQFFFHMPSNTHPRFAKV
                     SFFVQCIVALGVTVLFVARTFHAGKVYNFDGHYFDFDAEEISRLVGVFIILYVVYYII
                     IAVFRAVIAKDQDRKVILYFLAFFIIATIPPSVTNILSRDGALDRGTHQTVIVACNVL
                     GFFGVMTVFLNTTRDRTTFMAKIISISIITFLTVFQGLNYFVLSDKEETYDELHRKDA
                     ALMLATPYRAADFRYAMVWSMKDAKEKPAFGSVPAVDLDFYKIEIANASILERIAALP
                     SSNPVEFKTDVTRLLEKSHPGFEGYKGLILSEANALKETEKSPAIAVLDRVHSLKRKI
                     LFRYNKISQIPDAAFRNKLPAFIAEQPLWFAPFGKAMLDFANKHQDLTGQQLKGEVLV
                     FLRPAERAGVRLYRKNKDSLDPEADQFVSYLKADLEHDQMFEVAYSYRMYRSFINPPA
                     LIMTGILIAVMLIVLFGFRLFFLGALVNPLNVLLGGVEKVNAGDMSVQVPIKVGDEIG
                     FLSRSFNGMVASIREARARLEEYAVGLEAKVEERTKELRETLTEVQKLKEQQDGDYFL
                     TSLLIKPLSTNRARSQNVHVDFFIEQKKKFQFRKWGEEIGGDICMSDNVKLMDKPYTI
                     FLNADAMGKSIQGAGGALVLGSVFESIIERTKLSEARQYPEKWVKNAFIELHKVFESF
                     EGSMLISLVLGLVDEMTGLMYYVNAEHPWTVLYRNGRAQFIEEELTFRKLGTQGLEGG
                     MSIQTFQLQPGDILIAGSDGRDDLLLNTKEDGERVISEDEHAFLKHVEAGKGELRKIY
                     DSIKTVGELTDDFSLVRIGYLETAKQTRTSSEKAMEFVSKARTFLIQDNLDEAQIALE
                     EAVKLDNTDTEALKELIKVCYNKRDFRKASDYADDYIYLRPAESEILYLAAICHKLAG
                     ELARSADLAESLKLREPKRPEVLTLLAQIYLEMNNIPRARAMVDLALNLDPASAAALK
                     AHHEIQSPR"
     gene            4157..6466
                     /locus_tag="PLG78_05830"
     CDS             4157..6466
                     /locus_tag="PLG78_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22662.1"
                     /translation="MSSRQLHWVIIAVTGLAFCVSPAPKQPASSVTSRLVVVAPPRNA
                     PDDLSAGIRSILEALLQSAGMPVLPSSGVAGLTDNALEPALMCARAGAQTCIVPEIAS
                     QGTGISIRIRWLDARGEVTSSTFNVTLRETSQGIQDAFVSMMDAASAKGWLETSDWSA
                     DRQLAASRMIRPQTETLQHFSRGLSLESTLPYMAMLEYRNALLIDPDFTLAYARLFYV
                     CYESNPYSQTAEVDLLTQTRVRMDTDNAVWLALAFRDLGQNALRKGWLVTSDAYFRRS
                     LAILANIKKETSIPGSIALHGLGDLGLKNRNSINALYHTNSAQQALESVGAQNSSLYL
                     SGLLRLGAGYLLDNQTSLAVYTYEKARDLAGRLNAADSPEAAIIDANLGLARIRESNY
                     VGAAALYESALARLRKTGYVNTELYLTILGHLGQAQRFMGNISEAEATFERVITGSKI
                     LGLNESMIQAEAMYSTGALSMVRGDFARAQVLMISAQMTMMRLGNQRNAADHVALDLM
                     PVKQQAGMTLEEARLLSSYCGAFSYASHSRTVQARTYEGRLDDTNVFLRDLLDVRKSN
                     DAALNHLREKFATVPDPSGRDTAFVDIGPAIANMLTPGVTAVSLARDFPGMQVVALDL
                     PEQVEIFRTTVAAYLRSRVTAFPNMFILSGNGIQPMMDQFQNNANWFSQKQPRIPAGM
                     PLVIRAANSVDIYESWETNAKAMDRIAKDFKASAILYLFNRSILFKPAGSDSFRIIGV
                     LSPAGFDHMFETFNRQGDSAYTIFPDAVR"
     gene            6476..8290
                     /locus_tag="PLG78_05835"
     CDS             6476..8290
                     /locus_tag="PLG78_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22663.1"
                     /translation="MIREKFEEIRKSLGNLPGAFRIVWEADSGSTIRLASLTVIAALL
                     PVTQAYIGKLIVDSVVKAVATHTPGIEALREILPYLLTELALFAAGSFLAQTRRLAEQ
                     VLDQRAGHHITEQIIRKALTLEIRYFEDSKFYDNMQNARREAEYKALAIINNSFLLVQ
                     NTITLFSFVFILFAMSPIIALVLFGATIPSFAVQTRFSKMKFRMQSWRSPETRMITYL
                     EQILTMNTTVKEIRLFGLGDELLNRFNSIFHKIFTEDMSLAKKRLLFSYLWGLLSTLS
                     FYGCYAWIIYLTVLQRITLGDMTLYLGAVRQSQGSFQGILDNVSRLYENGLFMQNLFS
                     FLSLPGMNPTPSSKNWPATDTPGIHFDNVTYRYPDADLPALENFSLHIKPGERFAIVG
                     ENGSGKTTLIKLLSRLYEPADGAIRLDGVDLRDMSLDQIYARMGVIFQDFVRYQLSVR
                     ENIGFGDIAHLSETERILGAVHDAGADSLLADMPEGLETSLGHQFRRGRELSGGEWQK
                     IALARSFMRSAEILILDEPTSALDAQKEFEIFERFRSLTAGRTAILISHRFSTVRMAD
                     RIAVLEHGKLVELGSHDELMKLGGKYARLFELQARGYR"
     gene            complement(8292..>8911)
                     /locus_tag="PLG78_05840"
     CDS             complement(8292..>8911)
                     /locus_tag="PLG78_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22664.1"
                     /translation="WFWKGKELIAFARITVVGRAFSGISYWLVLGLLPDAEGSLMILF
                     RTYLTVQGSIDLGSAAITAFSVLQKKEADGKDRSLSLEEKNRFVFAIYMTLLSVWILA
                     DSFSFLKFFHLPPTSFSGWTAFRFGPIHILAAQIFILAIYNFTAVRFRLTPLIEAGMR
                     GGMVTCIFFVILVLFGVLHPITLLLPGADLISVAVIFFARLRKRQ"
BASE COUNT         2365 a         2271 c         2168 g         2107 t
ORIGIN      
        1 cggagaaaac gtagagcccg tcccgatcga gaacaaactg gtggaatccc agtacattga
       61 tcaatgcatg gtcgtgggtc aggatcaaaa attcctggcg gtcctcgtag ttccttccct
      121 cgacggtttc gcacgtggcg gcctgaaagc aaaaacactg tccgaactcg cttccaatga
      181 agaggcaggc aaagtcatca ctgctgaaat caaacgaatc gtatccgccg accagggctt
      241 caagagcttt gaaagagtgc aggacttcag attcataacc aagccatttg aagtgggcga
      301 tgagatgacc aacctcttca aactgaaacg tcatgtgatc acggagaagt ataagaaaga
      361 aatcaattcg atgtataagt agttcgctcg aagtacaata gttccgttca aaaatagacc
      421 gtttggaata gaaggaggat ctatgtttgg cctgactcaa ctcggaatcg ttcacacact
      481 gattgcgctg atcgcagtcg gaactggcgc tttccttctc atccgggaag gaagaatttc
      541 acccgccggc ctggcaggta agatctatgt atttgcaact atcgtcgcat gcatcactgc
      601 tcttgccatc taccagcatg ggggctttgg aaagggtcac gtactcgcca tactcactct
      661 cgtcgtgctc ggtgtagcaa cggccgccgg cagaggcatg tttggcggcg tatcaaaggc
      721 agtggaaact gtatcctatt cagccacatt tcttttccac tggattccag gcgtatcgga
      781 agcaacgact cgccttcctc tgggttcgcc attgatgaat ggtccggaag atccggcgct
      841 caagacaatc ctcggagttc tcttcgtgct cttcgtagtt ggatccgttc tgcaagtgcg
      901 aagcctgcga aaatcgtaac agatcgcgcg aaataaattc gtttagtttg acaatgtgag
      961 gatgcccggc gcacactagt gcaatgtcgg gcaatctgtt cctgaacttc ttctcgattg
     1021 gagcgctcat tccgctcctc ttcgtaatgt tcatcgcgct cctcctcatt cgcatccgcg
     1081 acaaagggga atccacgtgg tggcttgctt ctttcgccat cgtctattgc ctttttatgc
     1141 ttccgtactt tccggcatcc gtcctttttc atccggccgg cgcctatcac agatggttca
     1201 cggttccctt tgtactactt acttttacat tcctggccca attcttcttt cacatgcctt
     1261 ccaatacgca tccgcgcttc gcaaaagtct cattcttcgt tcaatgcatc gtggctctgg
     1321 gagtaacggt cctctttgtt gcaaggactt tccacgccgg caaagtctac aactttgacg
     1381 ggcactattt tgacttcgat gcagaagaaa taagtcgatt ggtcggagtt ttcataatcc
     1441 tttatgtagt ttactatatc atcatcgccg tatttcgcgc agtgatcgcc aaagaccagg
     1501 acagaaaggt catcctgtac tttcttgcgt tcttcatcat cgcaaccatt ccgccctctg
     1561 ttacaaacat actcagccgc gacggagcac tggatcgcgg cacgcaccag acagtcatcg
     1621 tagcgtgtaa cgtacttgga ttctttggag tgatgaccgt gttcttgaac acgaccagag
     1681 atagaaccac attcatggcc aagatcattt ctatcagcat catcacattt ctcacagtat
     1741 tccagggttt gaattacttc gtgctctctg ataaagagga aacttacgac gaactgcatc
     1801 gtaaggacgc agccctgatg ctcgccactc cttacagggc agcggatttc agatatgcca
     1861 tggtatggtc catgaaggac gcaaaagaaa aacctgcgtt cggaagcgtc ccggcagtgg
     1921 acctggactt ctacaaaatt gaaatcgcaa acgcatccat tctagaaaga atcgctgctc
     1981 tcccctcttc taatccggtc gaattcaaaa cggacgtgac cagactcctc gaaaaatcgc
     2041 atccgggatt tgaaggatac aaaggcctca tactctccga agcaaacgca ctcaaagaaa
     2101 ccgaaaagtc gcccgcaatc gcagtcctgg atcgcgttca ttctctcaaa cgtaaaattc
     2161 tttttcgcta caacaaaatc tcacaaattc cggacgctgc ttttagaaac aaacttcctg
     2221 cgttcatagc cgagcaacca ctgtggttcg cgccgttcgg aaaagcaatg ctcgatttcg
     2281 caaacaagca ccaggacctg acagggcaac aactcaaggg ggaggtgctc gtattccttc
     2341 gccccgcgga aagagcgggc gtacgcctct accgcaagaa caaagactca ttggatccag
     2401 aagcggatca gttcgtatct tacctgaagg cggacctgga acatgaccag atgttcgaag
     2461 tcgcctactc ctatagaatg tatcgcagct ttatcaaccc gcccgcattg atcatgactg
     2521 gaatcctgat agcggtcatg ctgattgttc tttttgggtt tcgtctattc ttcctgggcg
     2581 cactggtcaa tcccctcaac gtcttgcttg gcggcgtaga aaaagtaaat gcaggcgaca
     2641 tgtctgtgca ggtgcccatc aaagtcgggg atgaaatcgg attcctatca agatcgttta
     2701 acggcatggt tgcctctatt cgagaagcac gcgccagact ggaggagtac gctgttggcc
     2761 tcgaagcaaa agtagaggag cgaaccaagg agctgcgcga aactttaaca gaagttcaga
     2821 aactaaaaga acagcaggac ggagactatt ttctgacttc gctattgatt aagcctttat
     2881 ccaccaatcg cgccagaagc cagaatgttc atgtggattt ctttatagaa caaaagaaga
     2941 aatttcagtt tagaaaatgg ggagaagaga tcggcggaga catatgcatg tcggataacg
     3001 tcaaactcat ggacaagccg tataccatct tcctgaacgc agatgcaatg ggcaaatcca
     3061 tccagggagc aggcggggcg ctggttcttg gttctgtttt cgaatcaatc attgagcgca
     3121 cgaaactctc ggaggccaga caatacccgg aaaaatgggt aaagaatgca ttcattgagc
     3181 ttcacaaagt tttcgaaagt tttgaaggaa gcatgctcat ctctctcgtg ctgggtctcg
     3241 tggacgaaat gacaggtctt atgtactacg taaatgcgga acatccatgg accgtgctgt
     3301 acagaaatgg acgcgcgcaa ttcatcgagg aagagctcac attccggaaa ctgggcacgc
     3361 aaggactcga agggggaatg tctatccaga cgtttcaact ccaaccgggg gacattctga
     3421 ttgctggctc tgacggaaga gacgacctac tgctcaacac gaaagaggac ggagaaagag
     3481 tcatcagtga agacgaacat gctttcttga aacacgtgga agcgggaaaa ggggaactga
     3541 gaaaaatcta cgattcaatc aaaacggtgg gtgaattaac ggacgacttt tctctcgtgc
     3601 gaattggcta tctggagact gcgaagcaaa cacgcacgag ttctgaaaag gcaatggaat
     3661 tcgtttcgaa agccagaacc ttcctcatcc aggacaacct ggacgaagcg caaatcgccc
     3721 tggaagaagc ggtaaagctg gataacacag atacggaagc gctcaaagaa ttgataaaag
     3781 tttgctacaa caaaagagac ttccgcaagg catccgacta cgcagacgat tatatttatc
     3841 ttcgtcccgc tgaatcggag atactctacc tggcggccat ctgtcacaag cttgcaggag
     3901 aactcgcacg atctgcggat cttgcagaaa gtctgaaact gcgcgaacca aaaaggccgg
     3961 aagttctaac actcctcgct cagatttacc tggaaatgaa caacatcccg cgcgcaagag
     4021 caatggtaga cctggcactc aacctggatc cggccagtgc ggcggctctc aaggcgcacc
     4081 atgagattca gtctccgcgt taattgcgat ttgcaaaaca ggtgacgaat cctgaccgat
     4141 atgcgcacaa gggtgaatgt catcgcggca acttcattgg gtgatcatcg cggtgactgg
     4201 tctggcgttc tgcgtttcac cggcaccaaa gcagccagct tcttctgtca catcgcgcct
     4261 cgtagtcgtt gctcctccgc gaaacgcacc tgacgattta tccgcaggca ttcgctctat
     4321 cctcgaagct cttcttcaat cagcaggaat gccagttctc ccttcgagcg gagtggcagg
     4381 actcacagac aatgcgctcg aaccagcact gatgtgcgct cgcgcgggcg cgcagacttg
     4441 cattgtcccg gaaattgcct cgcagggcac aggaatctct attcggattc gatggctcga
     4501 cgcacgagga gaagtcactt ccagcacatt caacgtaacg ttgcgcgaaa cttcgcaggg
     4561 gattcaggac gcgtttgtat ccatgatgga cgcagccagc gcaaagggtt ggctcgaaac
     4621 atcagactgg tccgcggata gacagctggc cgcttcgcgc atgattcgcc cacagacgga
     4681 gacgctgcaa catttttcca gaggcctgtc actcgaatct actctgcctt acatggccat
     4741 gctcgagtat cgcaacgcgc ttttgattga tccggatttt actcttgcct atgcgagact
     4801 gttctacgtt tgctatgaat caaatcctta ctctcaaaca gcggaagtcg atctactcac
     4861 tcagactaga gtgcgaatgg atacggataa tgcagtgtgg cttgctctgg ccttccgcga
     4921 tctgggtcaa aacgcactga gaaagggatg gctggtcact tccgacgctt actttcgacg
     4981 ctctcttgcc atccttgcaa acattaagaa agaaacttct atcccgggat caatcgcctt
     5041 acatggatta ggtgatcttg gtctgaaaaa tagaaacagc atcaacgctc tctaccatac
     5101 gaacagcgcg cagcaggcgc tggaaagtgt tggcgcgcag aattcatctc tgtatttgtc
     5161 cggtctcctc aggcttggcg cgggctatct actggacaat caaacatccc tcgctgtata
     5221 tacatacgaa aaggcgcgcg atctggcagg acgactgaac gcggcggata gcccggaggc
     5281 tgccatcatc gatgcgaatc tgggacttgc acgcatacga gaatccaatt atgtcggagc
     5341 ggccgcactg tatgagtccg cactggcgcg tcttcgcaaa acaggatacg tgaacactga
     5401 attgtatctg acgatccttg gacatctggg tcaggctcag agattcatgg gcaacatatc
     5461 ggaagcggag gcaacttttg agcgtgtcat tacaggcagc aaaattcttg gattgaatga
     5521 gtccatgatc caggcagaag caatgtattc gacgggcgct ctgtccatgg tccgcggtga
     5581 ctttgcccgg gcccaggtcc tgatgatatc cgcacagatg accatgatga gactgggcaa
     5641 tcaaaggaat gcggcagatc atgttgcgct tgatttaatg ccagtcaaac aacaggcggg
     5701 aatgacactg gaagaagcac gccttctttc ttcctattgc ggtgcattta gctacgcgtc
     5761 tcactcacga acagtgcagg ccaggactta tgaaggaaga ctcgatgaca caaacgtctt
     5821 ccttagagat ctactcgatg tgcgaaaatc caacgacgca gctttgaatc atctgcgcga
     5881 aaaatttgcg actgttccgg atccatccgg tcgcgacaca gcgtttgtgg acattgggcc
     5941 agccattgcc aatatgctaa ctcctggagt cacggctgtc tctctagcgc gcgactttcc
     6001 aggcatgcaa gtagtcgcac tcgaccttcc ggagcaagtg gaaatatttc gcaccactgt
     6061 tgcagcttat cttcgatcca gagtgacggc cttcccgaat atgtttatac tatcagggaa
     6121 cggaatacag ccgatgatgg atcaattcca gaacaatgcc aactggtttt cgcagaagca
     6181 acctcgaatt ccagctggca tgcctcttgt gattcgagca gccaattccg tggatatcta
     6241 tgaaagctgg gaaacaaacg cgaaggccat ggacagaatt gctaaagatt ttaaagcttc
     6301 tgccatcctc tatcttttca ataggtccat tttattcaaa ccagcaggct ctgattcatt
     6361 cagaatcatt ggagtacttt ctcctgccgg atttgatcac atgtttgaaa cgttcaatcg
     6421 ccagggagat tccgcttaca cgatctttcc tgacgcagtg cgataaggca atctgttgat
     6481 ccgcgaaaag ttcgaagaga tccgcaagtc cctcgggaat ttgccgggcg cattccgcat
     6541 tgtctgggaa gcagactcag gcagtacgat tcgccttgca tctcttacag tcattgcagc
     6601 gctccttccc gtaacgcagg cctacattgg caaactcatt gtagacagtg ttgtaaaggc
     6661 agtggcaacc cacactccgg gtatcgaagc tcttcgagag attcttccat acctgcttac
     6721 ggagctcgca ctcttcgcgg ctggatcttt tctcgcacag acaagaagac tcgcggagca
     6781 ggtcctggac cagagggccg gtcatcacat cacagagcaa atcatccgca aagcgctgac
     6841 gctcgagatt cgctacttcg aggattccaa attttatgat aacatgcaaa atgcaaggag
     6901 ggaagcagaa tacaaggctc tcgccataat caacaattcc ttcttgctgg ttcaaaatac
     6961 aatcactctg ttttcgttcg tgtttattct tttcgcgatg agtccgatca tcgccttagt
     7021 gctgtttggt gctaccattc cttcttttgc agtccagact cgcttcagca aaatgaaatt
     7081 ccgaatgcaa agctggcggt cccctgaaac cagaatgatc acctatctgg aacagatact
     7141 gaccatgaac acaactgtga aggagattcg attgttcggc ctgggcgatg agttgctgaa
     7201 tcggttcaac agcatcttcc acaaaatctt cacggaagac atgtctcttg cgaaaaagag
     7261 gcttttgttt tcctacctgt ggggactcct ttccaccttg agtttttatg gatgctacgc
     7321 atggatcatt tatctcacgg tactccagag gatcactctg ggtgatatga cgctctatct
     7381 tggagccgtg cgacaatccc agggcagctt ccagggcatc ctggacaacg taagcaggtt
     7441 gtatgagaac ggcctcttca tgcagaatct attttcgttc ctcagcctac caggcatgaa
     7501 tcctacacca tcgagcaaga actggccggc cacggacacg cctggaattc actttgacaa
     7561 tgtcacctat cgttatcccg acgcagatct acccgcgctg gaaaacttca gccttcacat
     7621 caaaccaggc gaacgctttg cgattgtagg cgaaaatgga agcggtaaga caaccctgat
     7681 caagctgcta tctcgcctgt acgaacctgc agatggagcg atccgactcg acggagtgga
     7741 tcttcgcgat atgagcctgg atcaaatcta tgcgcgaatg ggagtcatct tccaggactt
     7801 cgtgcgttac cagttatccg tgagagaaaa cattggcttt ggagatatcg cgcatctttc
     7861 ggaaacagaa agaatcctgg gggcagttca tgacgcggga gcagacagcc ttctggcaga
     7921 tatgcctgaa ggactggaaa catctctggg acatcagttc cgtcgaggta gagagctttc
     7981 aggcggcgaa tggcagaaga tcgcgctggc cagatctttt atgcgaagcg cggaaatact
     8041 aattctggac gagccaactt cggcgctcga tgcgcaaaag gagtttgaga tctttgaaag
     8101 attcagatcc ctcactgcag gtcgaacggc cattttgatt tcgcatagat tcagcactgt
     8161 gcggatggcg gatcgcatcg cagtgctcga acatggcaaa ctcgtagaac tgggaagtca
     8221 cgatgaactc atgaaactcg gcggtaagta tgcgcgtcta tttgaacttc aagccagagg
     8281 atacaggtag cttactgtcg ttttcgcagt cgcgcaaaaa agatcacggc cacggaaatc
     8341 aaatcggctc caggcaaaag aagagtgatt ggatgaagca ctccgaaaag cacaaggatt
     8401 acaaaaaaaa tgcaagtaac cattccgcct ctcatgcccg cttcaatgag gggcgtcagc
     8461 ctgaagcgaa cagcggtgaa attgtaaatc gcaaggatga aaatttgcgc cgccaggatg
     8521 tgaatgggac caaatcgaaa cgcagtccaa ccagaaaatg aagtaggcgg aagatgaaag
     8581 aatttcagaa aagaaaagct gtctgcaaga atccacacgg agagcaaagt catgtagatc
     8641 gcgaaaacaa atcgattctt ctcctcaaga gatagcgaac gatcttttcc atccgcttcc
     8701 ttcttctgga gaacagaaaa cgcggtgatc gcagcacttc caaggtctat gctgccctgc
     8761 acagtcaaat acgtgcgaaa cagaatcatc aaactgcctt ccgcgtctgg aaggaggcca
     8821 agcacaagcc agtaggaaat acccgaaaac gcgcgaccca caactgtgat gcgcgcaaat
     8881 gcaatgagtt cctttccttt ccagaaccag a
//
LOCUS       DAOQJL010000092         8908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4191_length_8908_cov_4.825709, whole genome shotgun sequence.
ACCESSION   DAOQJL010000092 DAOQJL010000000
VERSION     DAOQJL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_8908_cov_4.825709"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLG78_05845"
     CDS             <1..299
                     /locus_tag="PLG78_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE22665.1"
                     /translation="NINPPDIRFLFEALPEITFRDIPDRKVTEFNITSQNRTGLLFHI
                     TRIFAEEGISILRGTIKSEEDTAKDRFFLQQQGECISEESKSRLSAKITGQPAS"
     gene            complement(317..1144)
                     /locus_tag="PLG78_05850"
     CDS             complement(317..1144)
                     /locus_tag="PLG78_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22666.1"
                     /translation="MPSDKYIPIESVEDIDVLKLSPRDLDKRYIDRNGNRYALRFNMK
                     TRRPEVIQIVSNRLEARRLQQTILLEKRKPEKSQATTAESVSLPPPVFVPDEEAFHES
                     RFVDECVSELPKLKESQQASVAKIRQSRVFETVQSAEFLDLMRDIERDAWRKTDENVQ
                     QCREWISRPPSLHAALIRVQPDKRKILDSIPDDPVKLEAMRRWEWQDRLTDSFTRLND
                     FAVRLKQMLASVSDEELLKVPQNQRQFFKDSGASLDIIQDACEEKLARLDVWRKRFL"
     gene            complement(1191..2543)
                     /locus_tag="PLG78_05855"
     CDS             complement(1191..2543)
                     /locus_tag="PLG78_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNE22667.1"
                     /translation="MKQPMVKMMRHARAIISRRSARVVFGGLGLAGAVLAFGVFAQAP
                     GKGSDRGAEQLLRNMGGDVGQDLDPMIPAPGKDLTPDFGALERERQDAKGTVIRNRKQ
                     DQVATAGDKNRPERDRVRKDKGTTQKKKDDQKPVALAEERALPGFPTNLDPTPNPGFT
                     HDAPSFYRGIYLTNEVIRIPSMYEPWLKAAHDAGLNTLVIDVQLVGKDHKAIERLPPE
                     AFMKLAKSYGFYMVARVVCFQGGLETYPPSMALLDSVLRTAENAARMGFMEVQLDYIR
                     FADNRNLGYVTLNQRYRMIAGILRMATDRLRPYGIRVGADVFGRIVFNNDDIIGQKVE
                     LFSQHLDTLYPMLYPSHFYGELDRRKYPYKTIYEGVKASRARSGNTRIVAYIQGFPMR
                     VHESGLSLNDYIKDQIKASEDCGGDGFIVWHAANQYGPFFAAYAAYQKEKAARLASGK
                     "
     gene            complement(2618..3565)
                     /locus_tag="PLG78_05860"
     CDS             complement(2618..3565)
                     /locus_tag="PLG78_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22668.1"
                     /translation="MIDVNADRIRRKDLIKLVKEVSRLERKLAREQNNRTRTEATKDK
                     LQVLMKQIHLEIEESHAEIQKKNHELEILYADLEKEKKKSDDLLLNILPEEVADELKR
                     SGVVAPQHFDSVSVLFTDFKGFTQASEHLAPSQIVAELDACFTAFDEIIERHGLEKLK
                     TIGDSYMCAGGIPNEAPTHPIDTMLAALEIRDYMNRLKIQRIEEGLPYWELRIGIHVG
                     PLVAGVIGRRKFAYDIWGDTVNTASRMESSGLPGMVNVSAALEQAVSPYFQTSLRGKI
                     PAKNKGEIEMHFVHRLKPEYSSDPAGIHPNRKVVCYRLS"
     gene            complement(3569..4711)
                     /locus_tag="PLG78_05865"
     CDS             complement(3569..4711)
                     /locus_tag="PLG78_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNE22669.1"
                     /translation="MIQAAVGYSDDVDSADAMREAIAQCRAQMQAPPKAAFLFASYEQ
                     DHDALLAEFNRAFPGLPLIGGTSTAQITSSGRYAEDSVALILFASDDVEFGIGLGRNL
                     SVDPLRAAREAVQQAKSNLSAPPTLAIAFPESLTTSGERIIEGLKAELGTAFPIFGGT
                     TSDSHTGKGTFQYFRTERLTDSLPVLLLSGNVRFSFAVRSGYKPIGKKRTATKVKGNR
                     VYELDGESLLDLYQKYLGDINLVSPEYPLAVFENGSDQFYLRTSQFFDAKDGCVIFDG
                     DIPEGAVMQFTDGTRSDLLDAAGSAVRSAIQAFPGQPRVALIFSCATRVLTLGTKVKE
                     EYAVLDSVRPGGCCAGGFYVYGEIAPLERGGPTCFHNATFVTLLLG"
     gene            4790..5206
                     /locus_tag="PLG78_05870"
     CDS             4790..5206
                     /locus_tag="PLG78_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22670.1"
                     /translation="MTFVEFTMSEGSMLVNPFALRAATPVTDGQGRTLHYMLEFVDGS
                     NLRIDNQRDMDLIHKYSAMFTHTRTAGKTVMTSSGSFHSVNVNIIQNYESVESKSGQI
                     RLVIITLNGGEKRIFDNDYDINVLQEWIRAQNVIVK"
     gene            complement(5213..5977)
                     /locus_tag="PLG78_05875"
     CDS             complement(5213..5977)
                     /locus_tag="PLG78_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE22671.1"
                     /translation="MLDLEIKDGIARITMNRAPLNALNPELISQMRKTLADLAKNREC
                     RALLIDSAIDSFFSNGLDPEAMLALDVPGRADVFRSFLSLARDLYAFPAPTLSAIAGH
                     AMAGGAVIGIATDFRFLAEGKYSYCFSEVKVGLTIPEFLLEMISQVTGPHHLRSLAML
                     AERFRPEECLAMGLADKIVPRENLLIESRKYLLSLSDRPLSSMRSVKENMHAGTLARI
                     DRFLESQEDTFFQFLGANFEEGLKAVLERRRPQFTD"
     gene            6089..7957
                     /locus_tag="PLG78_05880"
     CDS             6089..7957
                     /locus_tag="PLG78_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22672.1"
                     /translation="MARLLICTLLLISSSVLADTILLKNGQKIEGLIVYQDADVVHLQ
                     TADKTIVLQKNLVKRISYSPLQKPDAKKQDNEAEIAKEEKEKEQKEKAKKAEAERQRL
                     QQIQKEKEKQELARKERLKQEQAKRERERAMREKKEEEDRKLAEQKLAEEKKAADEKL
                     EAEKRETEKREAEKRAEEKKQADKLEAARLEEERKKQEEAERLARIEAEKKQKEAAEQ
                     ARLAKIEEEVRQKKEREAAEQKRIEDAKIAAEQKIKNPNYLLFGIAIGKGASKTSAGA
                     NALYNIESYSRFTGGYGLRTRYSASTSSDSSSSSSSSSSSSGLRSISGIHGASGFTSD
                     QTSASEANASNPFQAGSHKGGSLHLLYGIGMWRFVFDVERFKNKRIDRLEYGAGIAGP
                     PGASLGVVYGAFTGTTEQFNEVKIGTEYSLVRKDFLDVFVLGGGQRRAHNVAGETIQN
                     SILPGQSTVPTLLSGEKASTLMRGLYAGGGLELGQSWGLRTSIEVYSMRGNLSAKAIG
                     LGRFFTFTPGTGQIALPSVDQRGTESGLVLTAGLFIRILEKYYFRLDYSGDSGTFKES
                     KTTLFGLDYSSDPAAVLAGVILPGLYTSNPMLARSPAKVRYSSLTAGFSMRIDFAR"
     gene            8004..8405
                     /locus_tag="PLG78_05885"
     CDS             8004..8405
                     /locus_tag="PLG78_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE22673.1"
                     /translation="MIVQDYMTKDPVTVRADDPLSRVGEVFDVERFRHLPVVDASRNL
                     VGILSDRDLRNIQCAMEILETSVQGEQHVLVKDVMTATVRSVSAGDSLKSAAELFLSL
                     RVGALPVVEDGKLAGILSYTDVLRAFISLAK"
     gene            complement(8386..>8908)
                     /locus_tag="PLG78_05890"
     CDS             complement(8386..>8908)
                     /locus_tag="PLG78_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE22674.1"
                     /translation="IPKDNPFVGKGGLPEIFVFGLRNPWRFSIAPDGLIYAGDVGQDR
                     REEVSVIRPGDNLGWNIMEADICFRPNCSSVGTVTPIHSYGRDEGFSITGGYLYRGRS
                     IRRLKGCYVYGDFGSGVIRAFPVDRGRKTGDVFTVAKLRGNISSFGEDEAGELYVVDY
                     RSGSILKIISQEK"
BASE COUNT         2367 a         2265 c         2232 g         2044 t
ORIGIN      
        1 gcaatatcaa tccaccggac attcgctttc tcttcgaagc tcttccagaa atcaccttcc
       61 gcgatattcc ggatagaaaa gtaaccgagt tcaacatcac cagccagaat cgcactggtc
      121 tgctatttca catcacacgc attttcgcag aagaaggaat cagtatcctt cgcggaacga
      181 tcaagagcga agaggatact gcgaaggata gatttttcct gcagcaacag ggagaatgca
      241 tcagcgaaga atcgaaatcc aggctcagcg caaaaatcac aggccagcca gccagctaac
      301 caggtctgcg cgaaaatcaa agaaagcgct tacgccagac atccagtcga gccagcttct
      361 cttcacatgc atcctgaatg atatccagac tcgcaccgga atctttaaag aactgacgct
      421 ggttctgggg aactttaagc agctcctcat ctgaaacaga agcgagcatc tgcttgagtc
      481 ttactgcaaa gtcattgagt ctcgtaaaac tgtctgtaag gcgatcctgc cattcccatc
      541 ttcgcatggc ttcgagcttc actggatcgt ctggaatcga atctagaatc tttctcttgt
      601 caggctgtac gcgaatgagt gcggcgtgca aagaaggcgg acgcgaaatc cattctctac
      661 attgctgcac gttctcatct gtcttgcgcc atgcgtccct ttcaatatcg cgcatgaggt
      721 ccaggaattc tgcggattgt acagtttcaa atacccgcga ctggcgaatc ttcgcaaccg
      781 atgcctgctg gctttccttt aactttggca attccgagac acactcgtcc acaaatctgc
      841 tttcgtgaaa tgcttcttcg tccgggacaa acacgggcgg cggaagactc acagattcag
      901 ctgtggtggc ctgcgacttt tccggtttgc gcttttctaa caaaatggtt tgttgcaacc
      961 ttcttgcctc aagcctgttg gatacaattt gaattacttc ggggcgcctt gtcttcatgt
     1021 tgaagcgaag cgcataacgg ttgccgttgc gatcgatgta ccgcttatcg aggtcgcgag
     1081 gcgaaagctt caatacatca atgtcttcta cagactcaat cggtatgtat ttgtccgacg
     1141 gcatggcgtg actcaaacat gactgatcag aatttcaagc agtattgtgc ctatttaccc
     1201 gatgcgagcc tggccgcttt ttccttctga taggccgcat aggcggcgaa gaaaggaccg
     1261 tactggttgg ccgcatgcca tacaatgaag ccatctccgc cgcaatcctc ggaagccttg
     1321 atctggtctt tgatatagtc attgaggctg agaccggatt cgtgcactcg cataggaaag
     1381 ccctggatgt atgcgacgat cctggtgttg cccgagcgag cgcgggatgc ttttacgcct
     1441 tcgtagatgg tcttgtaagg atacttacgt ctgtcgagct ctccgtagaa atgagaagga
     1501 tagagcattg gatacaatgt gtccaggtgc tgggagaaca gttccacttt ctggccaatg
     1561 atatcgtcgt tgttgaacac gattcttccg aatacgtctg cgcccacgcg aattccgtac
     1621 gggcgaagcc tgtcggttgc catgcgaaga atcccggcga tcatgcgata gcgctgattg
     1681 agagtcacat atccaagatt gcgattgtcc gcgaaacgaa tataatccag ctgcacttcc
     1741 atgaagccca ttctggctgc attttccgca gtcctgagaa cggaatcgag aagcgccata
     1801 gaaggaggat atgtctcaag cccgccctgg aagcaaacga ctctggccac catgtaaaaa
     1861 ccataagatt ttgcaagttt catgaacgct tcgggcggaa gcctttcgat ggccttgtga
     1921 tctttcccaa cgagttgaac atcaatgacg agtgtgttga ggcctgcatc atgggctgct
     1981 ttcagccagg gttcgtacat ggaaggaatt cggatgactt cattggtcag ataaattccg
     2041 cgataaaaag aaggggcatc gtgcgtgaaa cctggattcg gagttggatc gagattcgtt
     2101 ggaaaccctg gcaaagcacg ctcttcggcg agtgccactg gcttctggtc atctttcttc
     2161 ttctgggttg tccctttgtc cttgcgtacg cggtcgcgct ctggccgatt tttgtcaccg
     2221 gcagtggcta cctggtcctg cttgcgattg cggatgactg tgccttttgc atcctgacgt
     2281 tcgcgctcga gagcgccaaa atcaggggtc aggtcttttc ctggagctgg aatcatggga
     2341 tccagatcct gtccaacgtc cccacccata ttgcgcagaa gctgctctgc gccccgatcc
     2401 gatcctttgc ccggggcctg ggcaaaaact ccgaaagcca gtacagcgcc ggcaaggcca
     2461 aggcccccga acaccactct ggcagatcta cgagaaatga tagcgcgtgc gtgacgcatc
     2521 atcttaacca tcggctgttt catataaaaa gagtgaaccg gagccagttt tttgtaagga
     2581 gccaggcggg aaagccaaaa caggaccctg gatcgactca ggaaaggcgg taacagacta
     2641 ccttgcgatt tggatgaatt cctgcaggat ccgacgaata ctctggtttc aaacgatgca
     2701 caaagtgcat ttcgatttcc cctttgttct tggcagggat ctttccgcgt agcgaagtct
     2761 gaaagtaagg cgacactgcc tgctcgaggg ccgccgatac gttgaccatt cctggcaggc
     2821 cagaggactc catgcgcgac gcagtgttga ctgtatcgcc ccagatgtcg taggcaaact
     2881 tgcgtcttcc aatcacgcct gcgacgagcg gtcccacgtg tatcccaata cgcagctccc
     2941 agtaaggaag tccttcttca attctctgga tcttcaaacg attcatgtag tcacgaatct
     3001 caagcgcggc gagcatcgtg tcgattggat gtgtgggagc ttcgtttgga attccacccg
     3061 cacacatgta gctgtctcca atggttttga gtttttcaag accgtgtctt tcaatgattt
     3121 catcaaatgc ggtgaagcat gcatccagct ctgctacgat ttgagaagga gcaaggtgtt
     3181 cggacgcctg agtgaatccc ttgaaatcag taaacagcac actcacagag tcaaagtgtt
     3241 gaggagcaac gactccggaa cgtttcaatt catccgccac ttcctccgga aggatattga
     3301 gaagtaggtc gtctgacttc tttttctctt tttcaagatc cgcgtaaaga atttcgagct
     3361 catgattctt cttttgtatt tcagcgtgtg attcttctat ttccagatga atctgcttca
     3421 tcaatacctg aagcttatct ttggttgcct ctgttctggt gcgattgttc tgttctcgcg
     3481 caagcttcct ctcaagacgc gaaacttctt tcaccaactt gatgaggtct ttgcgtctga
     3541 tcctgtctgc gttcacatcg atcatgatct accccagaag aagagtaaca aatgtagcat
     3601 tgtggaagca agtgggacca cctcgctcaa gcggagcgat ttccccatag acgtaaaatc
     3661 cgccggcgca gcatccgccc ggacgaacac tatccagaac tgcatactcc tctttgacct
     3721 tcgtgcccag agtaagaacc cgtgtagcgc aagagaaaat gagagcaacg cgcggttgac
     3781 ccggaaacgc ttgtatggcg gagcgaacgg cggatcctgc ggcatccagc aaatcgcttc
     3841 tagttccatc cgtaaactgc atcaccgccc cttctggaat gtcgccatca aaaatcacgc
     3901 agccgtcttt cgcatcgaag aattgagaag tgcgcaaata gaattgatcg ctgccgtttt
     3961 caaacaccgc gagaggatat tcaggcgaca ccagattgat gtctcccaga tatttctggt
     4021 agagatccag gagagattct ccgtccagtt catacacgcg atttcctttt actttcgtgg
     4081 ctgtgcgctt ttttccaatt ggcttgtagc cgcttcgcac agcgaaactg aatcgcacat
     4141 tgccagatag aagaaggacg ggaagtgaat cagtcagacg ctctgtgcga aaatactgaa
     4201 aggttccttt tccagtgtga gaatcagaag tggttccgcc aaaaattgga aacgcagttc
     4261 ccagttctgc cttgagtcct tctatgattc gctcaccgct cgttgttaga ctctcaggaa
     4321 atgcaatcgc aagagttggt ggagcgctca aattcgattt tgcctgctga actgcttccc
     4381 gcgctgcacg aagtggatct acagataagt ttcgccccag tccgattccg aactcaacat
     4441 cgtccgatgc aaaaaggatc aacgcaacag aatcttccgc atatctgccg gaggaagtga
     4501 tctgtgctgt ggacgtgcct ccgatgagtg gaagccccgg aaaggcgcga ttgaattccg
     4561 caagaagcgc gtcatgatcc tgctcataag atgcaaacaa aaacgctgct ttaggaggag
     4621 cctgcatctg ggccctgcat tgagcaatcg cctcgcgcat ggcgtccgca gaatcgacgt
     4681 cgtcgctgta acccacagca gcttgaatca tggaaaacag ggttgctaaa aattttgatt
     4741 cccgtcaagc cctcattcca taaaccggag gaaaagcggg cgctgaagta tgacattcgt
     4801 tgaattcacc atgagcgaag gttccatgct ggtgaaccca tttgcattgc gcgcggccac
     4861 tccagtcacg gacggccagg gcagaactct ccattatatg ctggaatttg tggatggaag
     4921 caaccttcgc atagacaacc agcgagatat ggacctgatt cacaagtaca gcgccatgtt
     4981 cacgcacacc agaacagccg gaaaaacggt catgacctcc tccggatcgt ttcactccgt
     5041 gaatgtgaac atcatccaga attacgaatc cgtggaatca aagagcggac aaattcgcct
     5101 cgtgatcata acactcaacg gcggagaaaa gcgcattttt gacaacgact atgacatcaa
     5161 tgtgcttcag gaatggatcc gcgcacaaaa cgtaattgtt aaatagtctc aactaatccg
     5221 taaactgcgg acgccttctc tccagtaccg ctttcaatcc ttcctcaaaa ttcgctccaa
     5281 ggaactgaaa gaaagtgtcc tcctgacttt ctagaaacct gtcgatcctg gccagagtgc
     5341 ccgcgtgcat gttctctttc acggaccgca tgctggaaag agggcgatct gaaagggaca
     5401 aaaggtattt tctggactca atgaggaggt tttccctggg cacaatctta tccgcaaggc
     5461 ccatggccag acactcctca ggacgaaagc gttctgcaag catggccaga gatcgcaaat
     5521 gatgcgggcc cgttacctgc gaaatcatct ccaggagaaa ctccggaatc gtcagaccaa
     5581 ctttcacttc agagaaacag taagaatatt tgccttccgc cagaaagcga aagtccgttg
     5641 caattccaat cacagcgcca cccgccattg catgacccgc aatggcagaa agagtaggag
     5701 ccgggaaggc ataaagatcc cgggccagag aaagaaaact tcgaaaaacg tccgcgcgac
     5761 cgggaacgtc cagggcaagc attgcttctg gatccaaccc gtttgaaaag aatgaatcaa
     5821 ttgcagaatc aatcaggagt gctctgcatt cccgattctt cgcgaggtcc gccagggttt
     5881 ttcgcatctg cgaaatcaat tcgggattca gcgcattgag aggcgcccgg ttcatggtga
     5941 tgcgtgcaat tccgtctttg atttctaaat ccagcatgcc cgactgagga accaacccgc
     6001 acaaaggcaa gcccaatcat aagaaaagtc ataggtccgt ccgtgcattc tgattgcaca
     6061 tacagggacc atccacaaga agcttcctat ggcacgcttg ctgatttgca cactgctcct
     6121 gatttcctcg tcggttcttg cggatacaat tctactgaaa aatggccaga agatcgaggg
     6181 cttgattgtt tatcaagatg ccgatgtagt ccacctgcaa actgcagaca aaacaatcgt
     6241 tctacaaaag aatctggtga aacgaatcag ctattctccg ctgcagaagc cagacgcaaa
     6301 gaagcaagac aacgaagctg aaatcgcaaa agaggaaaag gaaaaagagc aaaaagagaa
     6361 agcaaaaaag gcagaagcgg aaagacaaag actccagcaa atccagaaag aaaaagagaa
     6421 acaggaactg gcgcgcaaag agagactcaa acaggaacag gcaaagagag agcgggagcg
     6481 cgcgatgcga gagaaaaagg aggaagagga taggaaactg gcggaacaga aactagcgga
     6541 agagaaaaaa gcggcagacg aaaaactgga agcggaaaag cgcgaaacag aaaagaggga
     6601 agcagaaaaa cgcgcggaag agaagaaaca agcggataag ctcgaagcgg caagactcga
     6661 agaagaaaga aagaaacagg aagaagcgga acgactcgcc agaatagagg cggagaaaaa
     6721 gcaaaaggaa gcagcagagc aggcacgtct ggccaaaatt gaagaagagg ttcgccagaa
     6781 gaaagaaaga gaggcggctg aacaaaaacg aatcgaagac gcgaaaatcg ccgcagagca
     6841 gaaaataaag aatcctaact atcttctttt cggaatcgcc ataggaaaag gagcttcgaa
     6901 aacaagtgca ggtgcaaacg cactctacaa tatcgaatcc tattcccgtt ttacaggcgg
     6961 atatggtttg agaaccagat attcggcatc cacttcatct gactcaagtt cttccagttc
     7021 atccagctct tcaagttcag gactacgttc catttccggg atccacggag catccggttt
     7081 cacaagcgat caaaccagcg caagcgaggc aaacgcatcc aacccatttc aggccggatc
     7141 tcacaaaggc ggctcgctgc acctgcttta cggaataggc atgtggcgct ttgtattcga
     7201 tgtagaacga ttcaagaaca agcgcataga tcggcttgag tacggggctg gaatcgccgg
     7261 gccacctgga gcaagcctgg gtgtagtcta cggtgcattt acaggcacca cggagcaatt
     7321 caacgaggta aaaattggca ctgagtatag ccttgtgcga aaggatttcc tggacgtgtt
     7381 tgtgctggga ggtggtcaga gaagagcgca taacgttgcc ggagaaacca tccagaacag
     7441 cattcttccc ggtcagtcga cggtcccaac tctcctctcg ggagaaaaag cgtccacact
     7501 catgcgtgga ttgtatgccg ggggaggact cgagctcggt caatcgtggg gattgcgcac
     7561 gtctatagaa gtgtacagta tgagagggaa tctcagcgcg aaagcaatcg ggctgggtcg
     7621 atttttcaca ttcactcctg gcaccggcca gatcgctctt ccgagcgtgg atcagagggg
     7681 tacggaatca ggtcttgttt tgaccgccgg gttgttcata cgaatacttg aaaaatatta
     7741 ctttcgcctg gactattccg gagattccgg aacattcaag gagtcaaaga cgactttgtt
     7801 tggcctggac tactccagtg atcccgcagc cgttctcgca ggagtcatcc tgcccggtct
     7861 gtatacgagc aatccaatgc tggcccgctc ccctgcgaaa gtgcgataca gcagtctaac
     7921 cgcgggtttt tctatgcgaa ttgattttgc gcgatgacgc gcccatagct tgacagagag
     7981 aaagccgcgc atattcaagc attatgattg tccaggacta catgacgaaa gaccccgtca
     8041 cagtgagagc agatgatccc ctctccagag tcggagaggt ttttgacgtg gaaaggttca
     8101 ggcatctccc tgtagtggac gcgtcgcgca atctggtggg aattctatca gatcgtgatt
     8161 tgcgcaacat acagtgcgcc atggaaatcc ttgaaacgag cgttcaagga gagcagcacg
     8221 ttctcgtgaa agatgtgatg actgcgactg tgcgaagcgt ttctgcgggt gattctctga
     8281 aatctgccgc cgaattattc ctatctcttc gcgtgggggc gcttccagtg gtagaggacg
     8341 gaaaactggc gggaattcta agctacacgg atgtacttcg cgcgttcatt tctcttgcga
     8401 aatgattttg agaatcgatc cagagcgata gtcgaccacg taaagttcac cagcctcgtc
     8461 ttcgccaaag ctggatatgt tgccgcgcag tttcgcgact gtaaatacat cgccggtctt
     8521 tcgaccgcgg tccacaggga atgcgcggat cacgccggat ccaaaatcgc cgtaaacgta
     8581 gcatcccttg agcctacgaa tggatcttcc tctatataag tagccgcccg tgatgctgaa
     8641 tccttcgtct cttccgtaac tgtgaatggg tgtcacagtt cctacagagc tgcaattcgg
     8701 ccgaaagcaa atatctgcct ccatgatgtt ccaacccaga ttgtctccag gccgaatcac
     8761 actcacttcc tcgcgtcgat cctgtccaac gtctccggca tagatcaaac cgtctggcgc
     8821 aatgctgaat ctccacggat tgcgcagccc aaaaacaaaa atctcaggca atccgccctt
     8881 cccgacgaat ggattgtctt ttggaatg
//
LOCUS       DAOQJL010000093         8906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4193_length_8906_cov_5.635182, whole genome shotgun sequence.
ACCESSION   DAOQJL010000093 DAOQJL010000000
VERSION     DAOQJL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_8906_cov_5.635182"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLG78_05895"
     CDS             complement(<1..501)
                     /locus_tag="PLG78_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE22675.1"
                     /translation="MKEIQYDNQIVRWWVIGAIVWGLAAMLGGVILAFQLVYPELNMG
                     LPWISFGRLRPVHTNGAIFGFTLSIVFATFYYAIPRLLKTPMWSSKLSRVHFGLYTLT
                     IVLAVVTLVSGITQSKEYAELEWPIDVLIAAWWVIMGLNVFMTIKNREEQHLYAAIWF
                     FIATIVT"
     gene            679..1791
                     /locus_tag="PLG78_05900"
     CDS             679..1791
                     /locus_tag="PLG78_05900"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE22676.1"
                     /translation="METILQTVGISESALGKVKDLWRSGSIRLDLPVIDLQHIWLVAL
                     IQELSLETDGTRSPDAEKLKKLLLEISDFATEHFSLEEDLTTRFRYPEHPGHREQHKH
                     FIEVLNGKIADVRNGQTASINALVNFLLDWLTMHIQKEDKNYRDFLVRSNANLREYFQ
                     KLVNAREITIDRGQVALYNMVEGKSEVGEILNESILSNIIKIWNTHNLAVQIPIIDLQ
                     HIWLISMVVELDIASKTLGNTKRQKIFNKVIAGTVEYTREHFATEEKIMERFAYKNTQ
                     NHIKQHRSFVEMVHRRQQENETGDARAAAHLVEDLKEWLVSHIAIEDRNIYLGLKDQL
                     SEIQNYVRELINAGELVVKKKQVDMYNQVCGLKKAS"
     gene            1873..2151
                     /locus_tag="PLG78_05905"
     CDS             1873..2151
                     /locus_tag="PLG78_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22677.1"
                     /translation="MNLLELQKTVQEAIDKGARSVEQVHKQIANMPLEYLAKIAQLEG
                     PANQAKEFQTRTIGSIYDAIANINSKAGEIARDLLAKLPQGPGAPPKV"
     gene            complement(2155..2850)
                     /locus_tag="PLG78_05910"
     CDS             complement(2155..2850)
                     /locus_tag="PLG78_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE22678.1"
                     /translation="MFHSCSESCAECATMHETPFGTVPPSMLALVDEKKGTLNFKKGE
                     SIFHADDPAQAFYCISAGQVQLYRASRAKEQAFGVYGPGTWLGYRNALTGSAFDHSAR
                     CLTSVKVCKVDRAVLEGFSIRFPSFTKAIVRELASGWTESEKQVYNLGARNVKERLAE
                     YLLSLRKDTLAESHDFDFPLTRETLASLMGTTTESVIRTLSDFRSRGWIDYHDGKISL
                     SNERELEKLVVES"
     gene            complement(2914..3456)
                     /locus_tag="PLG78_05915"
     CDS             complement(2914..3456)
                     /locus_tag="PLG78_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22679.1"
                     /translation="MTEPKIARGTTLGFVPVIFYNLHAAYWIFRGGWENSLWACHVAS
                     LLIGAGMIFGTARLVLTGMLWLVIGNAFWILYLGTGGEFLPTSLLTHAGSFVIGLVGL
                     NRFGVVPGTWWRAALGLIPLHLAARFTPPAENVNLAHRVDQGWASVFPAHSTYVLFVF
                     ALCLVTFFVSERLFRKIKKG"
     gene            complement(3443..4159)
                     /locus_tag="PLG78_05920"
     CDS             complement(3443..4159)
                     /locus_tag="PLG78_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNE22680.1"
                     /translation="MIWLVVAFLILAGGELVAFVLGPSGFPDSIFRFLHLGQMVLLIV
                     ASFFVRAQLAARSAMELSEAAWNRAATLIQIGLVFSFVGDIINSGLIDLRSILKPQVL
                     ISIPFFATAHILYIRSYFIQTAFLSSRTIFLWVLWPFCAFALWKAVVDPSNTLIAALS
                     LPYSFMVSLMAISSIRMPMKYGLPGFTVTFGAFLFLLSDALIGWSITREHTRELSQVI
                     WSTYYTAQILILRSLYLDRT"
     gene            4257..4988
                     /locus_tag="PLG78_05925"
     CDS             4257..4988
                     /locus_tag="PLG78_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE22681.1"
                     /translation="MNVIKSILFWLFLGITCISLFPIALVIRIVTGPFDRRLRLLHRF
                     TSFWASLYTWLNPFWGVSILHKTTVDPEQTYVIVSNHQSLVDIFAFFRTFVHFKWVSK
                     SENFKFPFVGWNMRLNRYIEIKRGSNRSSAEMMKQCENTLRSGSSIFIFPEGTRSTTG
                     MLGKFKMGAFELAKRTGLPILPMVVHGSSNALPKGGLILSGIHRIRIHIIDAITADEV
                     NRLSADELCDLTRNRIESELALMQG"
     gene            4992..5492
                     /locus_tag="PLG78_05930"
     CDS             4992..5492
                     /locus_tag="PLG78_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE22682.1"
                     /translation="MRKLFAYILGTVMSIMVVFFVVGLVAPKEYTGSKTAQISAPPSR
                     VWAVLTDIDSLPKNRPEIARVEVLEKDGPRAKKWIEHTDMDGFIVFERVEEIQNKKLV
                     LKMVESSFGMTGTWTYELSGNEMTQVTVKENSQIASVPLRAAMTLMGRDANLKKEIQI
                     INKAAQ"
     gene            complement(5522..6895)
                     /locus_tag="PLG78_05935"
     CDS             complement(5522..6895)
                     /locus_tag="PLG78_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE22683.1"
                     /translation="MRLLWLTGISLTLFGTALSAREICNNACISGDCKNTVSLQVYTN
                     CDKYEGSFSGGSRHGPGEYTFANGDVYKGEFLNGNRAGKGIYFFKSGQATDYSLMFDL
                     DVNGTGTGILRANQKEIPCKILRGEALCGIGTKLRISEKMDPIAIVLYAGDSARIDRK
                     GEPIAVSSGDPILPGDSIRSGSDNVDLQFKDNIAVRAKPGSNLSFPREQERRIDLKEG
                     AVTVKFGQDSGSLAVTAPAARVDADDSTFAVQTTAQKTDVKVFEINKGKVTVAPAIPE
                     LSGKSDAEIRKDPELSKLQEKQRSLSTVLNVNQSGNLDRGTTATAEVKPFVPTVREKF
                     ESKVIVLADPEAVKKLSQDPGNAQAKIAMKQDYEKKLDRAASSVETDLSEAPVSTEED
                     VMRRYGLVEYVQMLDKKMYKGCVVAQAGDTLILHGLGAVRRIKVSDVNYIEYRHPGER
                     APEPSSP"
     gene            complement(6932..7870)
                     /locus_tag="PLG78_05940"
     CDS             complement(6932..7870)
                     /locus_tag="PLG78_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22684.1"
                     /translation="MRRFLTLVNIGMSSFARVFILLFASALHADTVLLVDGRLLEGKY
                     LGDRGDFIEWEQAGQIVKLESTNVVRIELAYPGVPVCFRRKGAKERVCQGKLYLLYGS
                     SVEIIGGPGDTKKETVKISDLSDLEFQRTTETQLLLPRIRPGAAIRLVIPEGSVQGSV
                     QSVEPGRVLVRDSQDQVVEVREEKIEQLQLPTSSGFLFSRLIPGLPQYRRSERIKSGA
                     IVGGIAFFGMSALWEQSQAQREVSRARANLLYISTGIGSRESVFAGHRKNAQILGFGM
                     LLTYGFHLTDEFVLSARSSRKSIQIGFSFPLALLLE"
     gene            7871..>8906
                     /locus_tag="PLG78_05945"
     CDS             7871..>8906
                     /locus_tag="PLG78_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE22685.1"
                     /translation="MKQVIPRLGGSLRLEIFIIYAVLAVSTIVFFSTIILENQSDLLL
                     DLFRYQSRDVTQELGQALRKEKFDAAKPESLTQELAKRQITSYYVINATGECLFPAKC
                     TTPPGATDTIRTLISEAALFKTDSHISLDPDSGTASFVLSLDDSQRLFLQGSLKVSAM
                     EARMSQVNRQLWLVLGWMVVFNVAFGIYVYRRIFSRVDKLKLASEKMATGSLDARVNW
                     KWRKDELDLLGRSFNAMAETIETQMKQISRLNLEMNEEMRIGREVQQVFLMQQEEITS
                     RGIAMYFQPLRQVSGDVYFFQTINYKDRTYEVFFLADATGHGVPAALITSILLLSLRE
                     ALASTLHPGKV"
BASE COUNT         2487 a         2259 c         2116 g         2044 t
ORIGIN      
        1 cgtaacgatc gttgcgatga agaaccagat cgcagcgtaa agatgctgct cttctctgtt
       61 cttaatcgtc atgaatacat taagtcccat gatcacccac catgctgcga tgagaacgtc
      121 aatgggccat tccagttctg cgtattcctt actctgagta atcccgctaa cgagtgttac
      181 taccgccaga acgatcgtaa gtgtgtacaa accaaagtga actcgcgaga gttttgatga
      241 ccacatcggc gttttcaaga gtcgtggaat tgcgtagtaa aaagtcgcaa acacgatgct
      301 caatgtgaat ccgaagatgg ctccgtttgt atggaccggt ctcagtcgtc caaatgagat
      361 ccatggaagc cccatgttga gttctggata aactagctgg aacgcgagta tcacgcctcc
      421 cagcatagca gccagtcccc acacgatagc tccaatgacc caccatcgaa cgatctggtt
      481 gtcgtattgt atttctttca tgccttgctc cgctgaagat ggtttaaatt tataccgatt
      541 ccggcccaaa ctgaaatgac agctgtcaac acggagttat gacaaaaatc agacaaactt
      601 ccgtgagaac gggtctcatt ctatacccgc tcccaaaaag gtaaaccacg attggcgagc
      661 ggaaaaaaac ctggcactat ggaaacaata ctgcaaacag tcgggatcag cgagtctgcg
      721 ctcggtaagg tgaaagacct ctggagaagc ggttccattc gcctcgattt gcctgtcatt
      781 gatctacagc acatctggct ggtagctctc atccaggaac tctctctgga aacagatggg
      841 acaagatcac cggacgctga aaaactaaaa aaactccttc tggaaatctc agactttgca
      901 acggagcatt tttctctgga ggaggacctg accacgcgtt ttcgttatcc ggagcaccct
      961 ggacacagag agcagcacaa acacttcatc gaagtgctca atgggaaaat cgcagatgta
     1021 agaaacggcc agacggcttc tatcaatgcg ctcgtcaact tcctcctgga ctggttgacc
     1081 atgcacatcc agaaagaaga caagaactac cgcgactttc ttgttagatc aaacgcaaat
     1141 ctacgggaat actttcagaa actcgtcaac gcacgtgaaa tcacaatcga ccggggtcag
     1201 gtggccctct acaatatggt agaagggaaa tcagaagtag gcgaaattct aaacgaaagc
     1261 atactcagca acatcatcaa aatctggaac acgcacaacc tggcggtgca aattccaatc
     1321 attgatctgc aacacatatg gttgatcagc atggtagtag aactcgacat cgcgagtaag
     1381 actctgggaa atacaaagag gcagaagatc ttcaacaaag tgatcgcagg tacggtcgaa
     1441 tatactcggg aacatttcgc aacagaagag aaaatcatgg aacgattcgc ttataagaat
     1501 acgcagaatc acataaagca acatcgatcc ttcgtagaaa tggttcatag aagacaacag
     1561 gagaatgaaa caggcgacgc tcgcgcagcg gcgcacctgg tggaagactt gaaagaatgg
     1621 cttgtgtctc atattgcgat tgaagacaga aatatttacc tgggattgaa agatcagctt
     1681 tcggaaatcc agaactatgt cagggaactc atcaatgcag gagagctggt ggtgaaaaag
     1741 aagcaagtgg acatgtacaa tcaggtctgc ggtttgaaga aagcatcata aatcacttgc
     1801 cggcacaatc cagaccgacc taatacgaca ccggccgggc aaccggtcgg aatattgaat
     1861 cggggaaatc aaatgaacct gctagaactg cagaaaactg ttcaggaagc catcgacaaa
     1921 ggtgctcgtt ccgtagaaca ggtacacaaa caaatcgcaa acatgcctct tgaatacctg
     1981 gcaaagattg ctcaactgga aggaccggct aatcaggcaa aagaattcca gacaagaacc
     2041 attggaagca tttacgacgc gattgcaaac atcaacagca aagcaggcga gatcgctcgc
     2101 gatttgcttg cgaagcttcc ccagggccca ggggctccac caaaagttta acgattaaga
     2161 ctcaacgaca agtttttcaa gctctctttc attcgaaagg gagatcttgc cgtcgtggta
     2221 gtctatccac ccgcgcgagc gaaaatcaga gagagtgcga atcacagatt cagttgtggt
     2281 tcccatgaga cttgccagcg tttcgcgcgt gagtggaaaa tcaaaatcat gactctccgc
     2341 gagagtatct ttgcgaaggg atagcaaata ctcagccagc ctttctttga cgtttcttgc
     2401 acctaaatta tagacttgct tttctgattc agtccaaccc gaagccagct cacgaacgat
     2461 tgcttttgta aaggatggaa aacggatcga gaatccttcg agcacagcgc gatccacctt
     2521 gcacactttc acagaagtaa gacagcgagc gctgtgatca aacgcactgc ctgtgagtgc
     2581 gttgcgataa cccagccatg tgcccggacc atacactccg aacgcctgtt ctttcgcacg
     2641 actggctctg tacaattgca cctgtcccgc agaaatgcag tagaatgcct gggctggatc
     2701 atccgcgtgg aaaatgcttt cccctttctt gaaattcagc gttccttttt tctcatccac
     2761 aagggcgagc atggaaggag gcaccgtgcc gaaaggcgtc tcgtgcatgg ttgcgcattc
     2821 agcgcacgat tcgctgcaag aatggaacat gaggccccca gtgtgaacac gccatccacg
     2881 ccgtcaataa atcatccaaa accatgatga aaatcatccc tttttgattt tgcgaaagag
     2941 tctctccgag acaaaaaacg tcacgagaca taacgcgaaa acaaacaata cgtaggtgct
     3001 gtgcgccgga aatacactgg cccagccctg atccactcta tgggccagat tcacgttttc
     3061 agcaggcggc gtgaaacggg cggcaaggtg gagaggaatc aatcccagag cggccctcca
     3121 ccaggttccc ggcaccactc caaagcgatt caacccaacc agtcctatca caaaacttcc
     3181 ggcatgagtc agaagagaag tcggcaagaa ttctccgccc gtgcccagat acaggatcca
     3241 gaaagcgttt ccaatgacca gccagagcat tccggtcaga acaagcctgg ctgtgccgaa
     3301 aatcattccg gccccgatca aaagggaagc aacgtggcat gcccagagcg aattctccca
     3361 gccaccgcga aagatccaat aggcagcatg caaattgtag aagatgacgg gaacaaagcc
     3421 cagcgtagtc ccgcgggcga ttttaggttc tgtcaagata gagagacctc aaaatcaata
     3481 tctgtgcggt gtagtatgtg gaccagatta cctgactcaa ttcgcgggta tgttcgcgcg
     3541 tgatgctcca gcctatgaga gcgtccgaaa gcaaaaacag aaaggcacca aatgtcacag
     3601 tgaaaccagg caaaccatac ttcatgggca tgcgaatcga agaaatcgcc ataagcgaaa
     3661 ccataaaaga atacggaaga gaaagtgcgg caatcaaggt gttcgatgga tccactacag
     3721 ctttccacag cgcgaaagca cagaaaggcc agagaaccca cagaaatatg gtgcgcgaag
     3781 agagaaatgc tgtttgaatg aaataactgc gaatatacag gatatgtgca gttgcaaaaa
     3841 atggaatgga aattaaaacc tgcggtttca gaatgctgcg caagtcgatt agaccgctat
     3901 tgatgatgtc tcccacaaat gaaaagacga gtccaatctg tatgagagtg gcggcccgat
     3961 tccaggcggc ctctgaaagc tccatagcag agcgggcggc cagttgagcg cgaacaaaaa
     4021 aggacgcgac gatcaggaga accatctgtc caagatgcaa gaagcgaaat atagaatcag
     4081 gaaagccgga aggcccgagc acgaaagcta caagctctcc gcctgcgaga atgaggaaag
     4141 caactacgag ccagatcatg tgctgattcg acggatcaag gcccttgcgt caagtatcct
     4201 tgctcttttt tgcaactggt tgccccactt tgagtcgaat ttagaggtga agattcatga
     4261 acgtaattaa gtctatacta ttctggttgt ttcttggtat cacctgcatc agccttttcc
     4321 cgatcgcact cgtgatccgg atcgtaacgg ggccgtttga ccgtcgtctt cgcctgctac
     4381 atcgcttcac ctctttctgg gcttcgctct acacctggtt gaatcctttc tggggtgtaa
     4441 gcatccttca caaaaccact gtagatcctg aacagactta cgtgatcgta tccaatcatc
     4501 agtcacttgt ggacatcttc gcattctttc gcacattcgt tcatttcaaa tgggtcagca
     4561 aatcagagaa tttcaaattc ccatttgttg gctggaacat gaggctcaat cgatacatag
     4621 aaatcaaacg gggcagcaat cgcagcagcg cagaaatgat gaagcaatgc gaaaacactc
     4681 tgcgcagtgg aagttccatc ttcattttcc cggaaggaac aagatccacg acaggaatgc
     4741 tgggtaagtt caagatgggc gcgtttgaac ttgcgaaaag aacaggtctt ccgattcttc
     4801 ctatggtggt gcacggttcg tccaatgctc ttccgaaagg cggtctcatc ctgagcggaa
     4861 ttcatcgcat tcgcattcac atcatagatg caatcacggc agatgaagta aatcgcctga
     4921 gtgcggatga gttgtgcgat ctcacgcgaa atcgtatcga aagtgaactc gctttgatgc
     4981 aaggatagtg tatgcgtaaa ctgtttgcct acatcctcgg aaccgtgatg agcatcatgg
     5041 ttgtattctt tgtagttggg ttggttgccc caaaagaata cactggatcc aaaaccgcgc
     5101 agatttctgc gccaccttcc cgcgtctggg ccgtcctgac ggatattgat agcctgccaa
     5161 agaatcggcc tgaaattgcg cgagtagaag tgctcgaaaa agatggcccg cgggcaaaga
     5221 agtggatcga acacacggac atggacggct tcattgtttt tgaaagagtg gaggagattc
     5281 aaaacaaaaa actggttttg aaaatggtag agagctcatt cggaatgaca ggaacctgga
     5341 cgtatgaact ttccggcaat gaaatgactc aggttaccgt gaaagaaaat agccagatcg
     5401 caagcgttcc tctccgcgct gccatgactc tgatggggcg ggacgcaaat cttaagaaag
     5461 aaatacagat cataaacaaa gccgcgcaat gaaatggcgg ccggacattc aacaatgatt
     5521 cctacggact ggaaggttca ggcgcccttt ctccgggatg cctgtattca atgtagttca
     5581 cgtccgatac cttaatgcgt ctcactgcac ccaatccgtg cagaatcagg gtgtctcctg
     5641 cctgcgcaac aacacatccc ttatacatct tcttatctag catctgcaca tattccacaa
     5701 ggccgtatcg ccgcataacg tcttcctccg tagaaaccgg agcctctgaa agatccgttt
     5761 ccacactcga agctgcgcga tccaattttt tttcataatc ctgcttcatt gcaatcttcg
     5821 cctgcgcatt tcctggatcc tgagagagtt tcttcaccgc ctcaggatct gcgaggacaa
     5881 tcactttact ttcaaatttt tcgcgaacag tggggacaaa aggtttcacc tctgctgtcg
     5941 cggtagtgcc acgatccaga tttccagatt gattcacatt caagactgtg ctgagacttc
     6001 tctgcttttc ctgaagtttt gatagttcag gatctttacg aatttccgca tcgctttttc
     6061 cactgagttc tggaatcgcg ggcgctacag tgaccttacc tttgttgatc tcaaagactt
     6121 ttacatctgt cttttgcgcc gttgtctgaa ccgcgaaagt agaatcatcc gcatctactc
     6181 tggcggccgg ggcagttact gcaagactcc ctgaatcctg tccgaatttg acagtaactg
     6241 ctccctcttt tagatcaatt cgcctttcct gttctctggg aaaggataga ttggatccag
     6301 gcttcgcgcg aactgcaatg ttgtctttaa attgcaaatc cacattgtcg gaaccggatc
     6361 gaatggaatc tcccggcaga atgggatccc cggaggatac ggcgataggt tcgcccttgc
     6421 gatcaatgcg cgcagaatct ccagcatata aaacaattgc gattggatcc atcttctccg
     6481 aaattctgag tttggtacca atgccgcata acgcttcccc cctcaaaatc ttgcatggaa
     6541 tttctttttg attcgcgcgg agaattccag tcccggttcc gttcacatcc aggtcaaaca
     6601 tcagggaata gtcggtggct tgtccgcttt tgaaaaagta gatacccttc ccggcgcgat
     6661 tcccgttcag gaactcccct ttgtacacgt ctccattggc aaaagtgtat tctccgggtc
     6721 catgacgact accgccagaa aaagaaccct cgtatttatc acaatttgtg taaacctgca
     6781 agctcacggt attcttacaa tcgccactga tgcatgcatt gttgcaaatt tctcgagcgg
     6841 aaagggcagt accaaaaaga gtcaatgata ttccagtcag ccagagcaat cgcatgggtt
     6901 catagaggac ccaacaacgg aatgggtcaa gtcattccaa aagcaaagcc agcgggaaag
     6961 aaaaaccgat ctgaatcgac tttcgagacg atcgagcgct cagtacaaat tcatctgtca
     7021 ggtgaaatcc atatgtaaga agcattccaa atcctagaat ctgcgcgttc tttctatgcc
     7081 cggcaaaaac agattcacgc gacccaattc cggtactaat gtacaacaaa ttcgccctgg
     7141 cccgagaaac ttctcgctga gcctggcttt gttcccagag cgcagacata ccaaagaaag
     7201 caattccacc aacaatcgcc cccgacttga tgcgttctga tcttctgtat tggggcagtc
     7261 ctgggatgag ccttgaaaac agaaaaccac tggatgtggg aagctgcagt tgctcgattt
     7321 tttcttcgcg cacttccaca acttgatcct gcgaatcgcg gacgagaacg cgtcctggct
     7381 ccaccgactg cacactaccc tgaacgctgc cctccggaat caccaggcga atggcagccc
     7441 ctggccgaat tcgcggaaga agcaactggg tttccgtcgt gcgctgaaac tctagatcag
     7501 agagatcaga aatcttaacc gtctcctttt ttgtgtcgcc cgggccgccg atgatttcca
     7561 cagagcttcc gtaaagaagg taaagctttc cctggcagac gcgctccttc gcgccctttc
     7621 ggcgaaagca aaccggtact cccggatagg cgagctcaat tcgcacgacg ttggtagatt
     7681 cgagctttac gatttgccct gcttgctccc actcgatgaa atcgccgcga tcacccagat
     7741 atttaccttc cagtaatcgt ccgtcgacga gaaggacagt gtcagcgtgc aatgcggacg
     7801 cgaacagtag gatgaaaacc cgtgcgaaag aggacattcc gatgttgacc agggtgagga
     7861 agcgtcgcac atgaagcaag tcattccgcg cctgggcggg tctcttcgcc ttgagatatt
     7921 cataatctat gcggtcctcg ccgtctcaac catcgtgttc ttctctacca tcatcctgga
     7981 aaaccagtct gatctgctgc tggatttatt ccgctaccag tcacgagatg tgacgcagga
     8041 actgggccag gctcttcgca aggagaagtt cgacgcggcg aaaccggaat cgctaacgca
     8101 ggagcttgcg aaacgccaaa taacgtctta ctacgtcata aatgcaactg gcgaatgcct
     8161 atttccggca aagtgcacca cgcccccggg cgccactgat acgattcgca cgctcatttc
     8221 ggaggcagcc ctcttcaaaa ctgacagtca tatatccctg gatccagact cgggtacagc
     8281 cagctttgtg ctcagcctgg atgattcgca acggctcttc ttgcaaggtt ccttgaaggt
     8341 atctgcgatg gaagcgcgta tgtcccaggt gaaccggcag ctctggctgg tcctgggctg
     8401 gatggttgta tttaacgtcg catttggaat ctatgtgtat cgcaggatct tctcacgcgt
     8461 ggacaaactg aaactggcga gcgaaaaaat ggctaccgga agcctggatg cacgggtgaa
     8521 ctggaaatgg agaaaggatg aactggatct cctcggtcgt tcgtttaacg caatggcaga
     8581 gacgatcgaa acacagatga aacaaatatc acgcctcaat ctcgaaatga acgaagagat
     8641 gcgaattgga cgcgaagtgc agcaggtctt cttgatgcag caggaagaaa taacgtcacg
     8701 cggcattgca atgtattttc aaccactcag acaggtcagc ggggatgtat atttctttca
     8761 aacaatcaac tacaaggatc gcacatacga ggttttcttt ctggcggatg caacaggaca
     8821 cggcgtgccc gcggcactca tcacttccat tctgctacta agtttgcgcg aagctctggc
     8881 atccacgctc catcctggca aagttt
//
LOCUS       DAOQJL010000094         8903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4199_length_8903_cov_5.130425, whole genome shotgun sequence.
ACCESSION   DAOQJL010000094 DAOQJL010000000
VERSION     DAOQJL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_8903_cov_5.130425"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLG78_05950"
     CDS             <1..265
                     /locus_tag="PLG78_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22686.1"
                     /translation="GGVIASGRTGNFVGPLQKLAFESTESESENENGESSGRPGDRTE
                     TRTFYFWGDVTDVQLVLTRDWTQVELSFDLPAPQTLSEDQRGH"
     gene            265..864
                     /locus_tag="PLG78_05955"
     CDS             265..864
                     /locus_tag="PLG78_05955"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNE22687.1"
                     /translation="MFTGIVESLGRVVAMVGDENQKTFTIESTFAQELIPGQSVSHNG
                     VCLTVEKIDAKASVYNVTLIRQTLERSSLGALRVSSNVNLERCVRADARLDGHVVQGH
                     VDSVGTIVLAENEPGQKRFRIEFPDKFASLIVPRGSICVDGISLTVANSGPGQVFDVC
                     LIPHTLEVTIASQWEVGTTVNLEFDIIGKYVKKWMDLRG"
     gene            887..1453
                     /locus_tag="PLG78_05960"
     CDS             887..1453
                     /locus_tag="PLG78_05960"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNE22688.1"
                     /translation="MFSKIKEVYSRIAPTRTFRIVRALGLALIIGGIWLQFIQPRLPG
                     KKDMPDFEPELAAETGKPNVLDWKNLRELNLKTNQPSAKLKKSDGQVVRIAGFMVPLE
                     DQAEQVSEFLLVPYPQACIHVPAPPPNQIVHVKMAAGKKANMVWWEPIWAEGKLRIVR
                     TANIYTESSFQLSGLKTEPYQDDYDAPH"
     gene            1459..2133
                     /locus_tag="PLG78_05965"
     CDS             1459..2133
                     /locus_tag="PLG78_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22689.1"
                     /translation="MMKKILIALVVIAAGLYWFTHRATSVNSTDAPGFKPLRDDALAQ
                     KYRPYLLASEFGKPQALYYRASRSGEKLYITYHYVWDREVNNAEGWKPWLSRNLYTGG
                     LALQRTMFGKGDIEMVSLVLDGEGKVESVVYETALGHSPSDFSVKHETIRTARPATPL
                     VFKVISWNHLFLLQEKPPDPSAWLDLDVEYFTQPLWEEYEMFKEKETVLKRNRAHEIY
                     ERAPAH"
     gene            complement(2084..3496)
                     /locus_tag="PLG78_05970"
     CDS             complement(2084..3496)
                     /locus_tag="PLG78_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22690.1"
                     /translation="MAMFVPPVLYLLSLLLVAYLLRDQDRQDDQYFLAGRTIGSLPAI
                     LSVVANETSVATVMIFPAAGFKSDFVLVWLCLGFILGRTLVAVFFMRKLYENHKLSIY
                     ETIGGTRTGSVPLAFAYVMAKYIANGARFYMAGYALQQLLGWNIAGWIVVVAMIVAVY
                     SLTGGIRAVVITDQIQGSVIYAMGLFLVAYLGYEIYQIGQAGYAHADALGILQRLRLT
                     DARASFTNSDYYLTLLLGGMVISIGSHGADQDMIQRILATKSARRASRSIFISGIGAS
                     IVILTYLAIGVLLREAGHGQLDAKSPLVDYIFHLNHPWLAGCFVVLLLSTGMSSLDSA
                     TNSTGAIWKFITKSNYPGILWSVFSLECLVISALLFALLGRSDFLDLALGSMSYIHGA
                     LIAILITYTFLPRLIHPASIVLAVAGSVGATIVLNLQVHSAWTVQILGSTFTALLLCM
                     AVGWISERVLSRRSRVPGSS"
     gene            3573..4973
                     /gene="mgtE"
                     /locus_tag="PLG78_05975"
     CDS             3573..4973
                     /gene="mgtE"
                     /locus_tag="PLG78_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNE22691.1"
                     /translation="MDKDPKELAREEEYSFRRDDPAAGDQIETLLDQHRDIELDELLV
                     ELYDADVAYLLQHLDQEQRERLLLRLSPERRGLVLLELDDEERKDLLETLTPRQVAEI
                     VQEQESDVAADIIEDLPEETTTEVISMLPQEDRRDVAELLSYPENSAGALMTKQFASV
                     LETSTVKQAIQSIRRATMQSDDIYTVYVVDAGGHYKGHLALDKLILASSRTRVKKIME
                     TELLPIPVNMDREEVAQMFTRYNFISVPVVDAQDVMVGRITVDDILEVVEEEASEDIL
                     RMGGVSADETLTSPIFQSSVKRNIWLFVNLLTAFLASSVVSLFEATIEKVVILATLMP
                     IVAGMGGNAASQTMAVIIRNIALGDLSPSGLPRALVREATMGAMNGILQGLITGLGVF
                     LFTGKIVLACVISAAMIINMMIAATAGTAIPLVLKKLNVDPAIASSIFVTTFTDVCGF
                     MSFLGLARLMLPYLVS"
     gene            4990..6573
                     /locus_tag="PLG78_05980"
     CDS             4990..6573
                     /locus_tag="PLG78_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE22692.1"
                     /translation="MTVNQYDAIVIGSGIGGLAVASILAQKEKKKVLVLERHFRAGGF
                     THEFTRKHYHWDVGVHYVGDMQEGGMMRRLFDVITDSKVDWEKMNEPFEKFVYPDFTF
                     PVFGNEERFIADLIEKFPAEEAAIRKYVADAKRAASWFGRHITMKSLPPFLDKLTHLV
                     DSLGLSSALMTTKQYFEEHFKDQKLRALLASQWGDYGLPPAESAFVIHAMIVVHYLAG
                     GYYPVGGAGKIAEAVQEILERTGGGILVSHEVTEVIVEGGRAVGVRARKLPARADSEQ
                     EFRAPVIVSDAGAWNTFLKLVPESVELPFRDSIRSFYDRVPVTTNVTLYLGLKDDPRK
                     LGFRGENYWIYSSYDHDQSFAQRSSWIESGKPPGAYLSFPSLKNRESKSHTAEIIAFT
                     DYGPFEKWKDASWKKRGEEYEAMKEKIAQSLIAYVDERLPGLKSIIDFHELSTPITNE
                     HFTAHPRGSIYGLACVPDRFREEKVAFCRAKTPIEGLFLSGADVSSPGIAGAMMGGFA
                     CLTQIVSGFTILSLFRSRK"
     gene            6570..7151
                     /locus_tag="PLG78_05985"
     CDS             6570..7151
                     /locus_tag="PLG78_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNE22693.1"
                     /translation="MRFAYLLLVASFALVSPVASRDLQNHELEIRYGPAQTCGTCAGQ
                     HLWQPMAKADALFQGTLNVSDDWILGGYGKASVYDHIFQGASGFLLGWNLGLVEARGY
                     AGIAYAGGVTERVRRGFFVYDGQTRATYDLGVSLRYPVADRWRLTLNYAHNSNGEDIG
                     MNFTGLRRKNPGIDSISFGVSYVLGQTPALLLP"
     gene            complement(7160..>8903)
                     /locus_tag="PLG78_05990"
     CDS             complement(7160..>8903)
                     /locus_tag="PLG78_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE22694.1"
                     /translation="EINARPITPALTLGIAREMGARLAAEIALRDSEEKWKLIAENVP
                     AFILLVDRQGKILFINHLFPGWKREEIIGKQIADFMPPEEQTLTPVRLADVFERGLEI
                     EYEQAAAGSPGEERHYASRIVPIRHEGAITAALLVATDITARKKDVARLEHQASHDSL
                     TDLPNRQFLIESALEAIGRAEKEGRQLALLLMDLDRFKEINDTLGHFTGDLLLKRIGP
                     RMESYLKSIGAEMARLGGDEFGILLPRIDEPAEAEGIAQTLHHLLREPFLLEGMNLEI
                     DCSIGVAVYPFHGQDPISLLRCADVAMYLAKKKSVGVAMYAPDQDRYSRRRLALLTEL
                     GLAIRESQLTLHYQPKYSLHDRKLVGVEALVRWHHPVHGMVSPGEFVPLAETGNLIIP
                     LTNWVFEEASRQWRVWKNAGHTVKIAVNLSVRNLMDEQYPLTVDAILKKHKVERSCFE
                     LEITESAIMADPERALSVLNRLHSLGVDLSIDDFGTGYSSLAYLSRLPIDALKIDLSF
                     VRQMMESERNMVIVNSTVRLAHTLGLIVIAEGVEDERTLNTLSDMKCDMAQGFFFSPP
                     RRAEDLTEIIARDL"
BASE COUNT         2236 a         2118 c         2366 g         2183 t
ORIGIN      
        1 aggaggagtg atcgcgtccg gtagaaccgg gaattttgtc gggcccctcc agaagctggc
       61 gttcgaatct acagaaagcg aaagtgaaaa cgaaaacgga gaatcatcgg gtcggccggg
      121 tgatcgcacg gagacgcgca ccttctattt ctggggggat gtgacagacg ttcagctggt
      181 cttgacgcga gattggactc aggtagaatt atcgtttgat cttccggcgc cacaaacgct
      241 atctgaagat cagcgagggc attgatgttt actggaattg tagaaagcct gggtcgcgtg
      301 gttgcgatgg tgggggatga aaatcagaag acattcacga ttgaatctac tttcgcacaa
      361 gaattgatcc cggggcaaag cgtctcgcac aacggagtgt gtctgaccgt agagaaaatc
      421 gatgcgaaag catctgtata caatgtgaca ttgatccgtc agactctgga acggagttcg
      481 ctgggagcct tgcgagtttc ttcgaatgtg aatctggagc gttgcgtgag agcggacgct
      541 cgtctggatg gacatgtagt gcaggggcat gtggattcag ttggaacgat agttctcgca
      601 gagaatgaac cggggcagaa acgtttcagg atagaatttc cggacaaatt tgcatcattg
      661 attgttccca ggggaagcat ttgcgtggat gggattagtc taaccgtggc gaattcgggt
      721 ccaggtcagg tttttgatgt ttgtttgatt ccgcacactc tggaagtgac gatcgcttcg
      781 caatgggaag tgggaacgac cgtgaatcta gaatttgaca tcattggaaa gtacgtaaaa
      841 aaatggatgg atttgcgagg ctgatctctt ttctctgaaa ccagagatgt tttcaaaaat
      901 caaagaagtc tattctcgca tcgcgcccac tagaaccttt cgcatcgtgc gcgcgctcgg
      961 ccttgcattg atcattggcg gtatctggct gcaattcatt cagccgcgtc tgccgggcaa
     1021 aaaggacatg cccgattttg agccagaact ggcggcagaa actggaaaac ccaatgtgct
     1081 cgactggaaa aacttacgtg agctgaatct taaaacaaat caaccatctg caaaacttaa
     1141 gaaatcggac ggacaggtgg tgcgaatcgc cggtttcatg gttcccctgg aagatcaggc
     1201 ggagcaggta agtgaatttc tactggttcc gtatccgcag gcatgcatcc acgttcctgc
     1261 accaccaccc aatcaaatcg ttcatgtgaa gatggcggcc ggcaagaaag caaacatggt
     1321 atggtgggag ccaatctggg cagaaggaaa gctgagaatc gttcgcactg cgaacattta
     1381 cactgaatcc tcttttcagt tgtcgggcct gaagacagaa ccataccagg acgactacga
     1441 cgctccacac tgagcactat gatgaagaag atactgattg ccctggtcgt gattgcggcc
     1501 ggcctgtact ggtttaccca tcgcgccacg tccgtaaact ccacggacgc accgggtttc
     1561 aaacctctgc gcgacgacgc actggctcag aaatacaggc cttatttgct cgcatctgaa
     1621 tttggaaagc cgcaggcact ctactacagg gcaagcagat ccggagagaa actatatatt
     1681 acgtatcatt atgtttggga tcgtgaggta aacaacgcag aaggttggaa accatggttg
     1741 agtcgcaacc tttatacagg tggtctggca ttgcagcgga ccatgtttgg caagggagat
     1801 attgaaatgg tatctctggt cctggacggc gaaggcaaag tggaaagcgt tgtatatgaa
     1861 actgcacttg gacacagtcc ttctgatttt tctgtaaagc atgagaccat tcgcaccgcg
     1921 cgaccggcta cacctctcgt attcaaagtg atttcgtgga accatctttt tctgctacag
     1981 gaaaaaccac cagatccatc cgcatggttg gacctggatg tggaatactt cactcaacct
     2041 ttatgggaag agtacgaaat gttcaaagag aaagaaaccg ttcttaagag gaaccgggca
     2101 cacgagatct acgagagagc acccgctcac tgatccagcc aacggccata cagagcagaa
     2161 gagcagtaaa agtagaaccc agtatttgaa cggtccacgc ggagtggacc tgtaagttca
     2221 atacaatcgt cgcgcccaca ctccctgcca cagcaagcac tatggaagcc ggatgaatga
     2281 gcctgggaag aaacgtgtag gtaattagaa tcgcgatgag tgcgccgtgt atgtaactca
     2341 tggagccaag cgcaagatcc aggaagtcag atcttccgag gagtgcaaac agaagcgctg
     2401 agatgacaag gcactcaaga gaaaacacag accagagtat tccaggatag ttggattttg
     2461 ttatgaattt ccagatagct cccgtagaat tcgtggcaga gtccagggaa gacatcccag
     2521 tggatagaag caacactaca aaacatcccg caagccaagg atgattcaaa tgaaagatat
     2581 agtctaccag aggtgatttt gcatccagct gcccgtgtcc cgcttcgcgc aatagcacac
     2641 cgatggctag atacgttaga atcacaatgc ttgccccgat gcctgaaatg aaaatggagc
     2701 gactggctcg gcgtgcgctt tttgtcgcga ggattctctg gatcatgtcc tggtccgctc
     2761 cgtgcgatcc aattgaaatc accataccac caagaagcag agtcagatag tagtcgctgt
     2821 tggtaaaaga agcgcgagca tctgtgagtc tgagtctctg caggattccc agagcgtccg
     2881 catgagcgta acctgcctgg cctatctgat agatttcata acccaggtag gctacgagga
     2941 atagacccat ggcgtagatt acacttccct ggatttgatc tgtgatgact actgcgcgaa
     3001 tgcctccagt cagactgtac actgcaacga tcattgccac cacgacgatc catccagcta
     3061 tattccagcc gagcaattgc tgcagcgcgt aacccgccat gtagaatctc gctccgtttg
     3121 cgatgtattt tgccatcaca tatgcgaacg caagagggac gcttccagtt ctagtcccgc
     3181 caatcgtttc gtatatggaa agcttgtgat tttcgtatag ctttcgcata aagaagactg
     3241 ccaccagggt tcgtcccagg atgaatccga gacagagcca caccagaacg aagtcggact
     3301 tgaaccctgc tgcaggaaaa atcatcactg tggcaacgga tgtctcattg gctacgacgg
     3361 aaaggatcgc aggaagcgat ccgatcgtgc ggcctgccag aaagtattgg tcgtcctgtc
     3421 tgtcctggtc tcgaagcaaa taagcaacca gtagcaggga aagcagatac aatacgggcg
     3481 ggacgaacat agccatggct ccatggatcg tttttcgaat taaaaggaaa ggaaatttga
     3541 cacgaccatc ctcggtcacg ggatgggcga agatggacaa ggatccaaag gagctcgcgc
     3601 gagaggaaga gtattcgttc aggcgggatg atccggctgc cggcgatcag attgaaactc
     3661 tcctggatca acacagagac atcgaactcg acgaattgct ggttgagctc tatgatgcag
     3721 acgtagctta cctgctccag cacctggatc aggaacaaag agagaggctt cttctacgtc
     3781 tttcgccgga aagacgcggt ctggtactcc tggaactcga tgatgaagag agaaaggatc
     3841 tacttgagac tctcactcct cgccaggttg cggaaattgt tcaggagcag gagtcagatg
     3901 tcgccgcgga catcattgaa gatcttcccg aagaaaccac aacggaagtc atctccatgc
     3961 ttcctcagga agacagacga gatgttgcgg aacttctttc ttatccagag aattcagccg
     4021 gcgcgctgat gaccaaacag tttgcttccg tgcttgaaac aagtactgtg aagcaagcaa
     4081 tccaatcgat tcgtcgcgca accatgcaaa gcgacgacat ctacactgta tatgttgtgg
     4141 acgcaggtgg acactataag gggcacctgg cactcgataa gttgattctt gccagttcgc
     4201 gcactcgcgt gaagaagatt atggagactg agctgcttcc gattccagtg aacatggacc
     4261 gcgaagaagt ggctcagatg ttcacgcgtt ataattttat ttccgtaccc gtcgtagacg
     4321 cgcaggatgt gatggttggc cgcattactg tggatgacat ccttgaggtt gtagaagaag
     4381 aagcgtccga ggatattctg cgcatgggcg gtgtttctgc ggatgaaaca ctcacttctc
     4441 ctatcttcca gtcgtccgtt aagcgaaata tctggctctt tgtaaatctg ctcaccgctt
     4501 ttctagcatc gagcgttgtg agtctatttg aagccaccat cgagaaggtg gtcatactcg
     4561 caaccttgat gcccattgtt gctggcatgg gaggcaatgc agcaagtcag actatggccg
     4621 taatcattcg caatatcgct ctgggcgatc tttcaccctc aggtcttccg cgtgcgctcg
     4681 ttcgcgaagc gacgatggga gccatgaatg gaattcttca gggactcatc accggactcg
     4741 gagtgtttct gtttacgggc aagattgttc tggcctgtgt gatttcggca gcgatgatca
     4801 tcaacatgat gatcgcggcc acagcgggaa cggccattcc attggttcta aagaaactca
     4861 atgtcgatcc ggccatcgca tccagcattt ttgttactac tttcacggat gtctgtggtt
     4921 tcatgagttt cctcggactg gcccgactca tgttgcccta ccttgtttcg taagacggag
     4981 gaagatcgta tgacggtaaa tcagtatgac gcaattgtaa ttggaagcgg gatcggcggt
     5041 ctcgcggtcg caagcatact ggcgcagaaa gaaaagaaga aagttctggt gctggaaaga
     5101 cactttcgcg ctggcggatt cactcacgaa tttacgcgaa agcattacca ctgggatgtt
     5161 ggcgttcact atgtcgggga tatgcaagaa ggcggaatga tgcgtcgatt gttcgatgtc
     5221 atcacggaca gcaaagtaga ctgggagaaa atgaatgagc cttttgaaaa gttcgtgtat
     5281 cccgatttca cgtttcctgt tttcggaaac gaagaacgat tcattgcaga tttgatagaa
     5341 aaattcccag cagaagaagc ggccattcgc aagtacgtag cggatgccaa gcgtgctgct
     5401 tcctggtttg gtcgccacat caccatgaag tctctgcctc cttttttgga taagctcacg
     5461 catctggtgg attcactggg gctttcttcc gcactcatga ccacgaaaca atacttcgag
     5521 gaacatttca aagatcagaa actccgcgca ctccttgcgt ctcagtgggg cgattatggc
     5581 cttccgcccg cagaaagcgc gtttgtaatc catgcgatga ttgtggttca ctaccttgca
     5641 ggtggctact atcctgtggg aggcgcgggt aagattgcgg aagcagttca ggaaatcctt
     5701 gagagaaccg gaggcgggat actcgtttct cacgaagtca cagaagtgat tgtagaaggt
     5761 ggacgcgcag tcggtgttcg cgcaaggaag ctcccggcgc gcgcagactc agagcaggag
     5821 tttcgcgccc ctgtgattgt ttctgacgcg ggtgcctgga atacttttct gaaacttgtt
     5881 ccggaatctg tggagctgcc atttcgagat agcattcgta gcttttatga tagagttccg
     5941 gtcacaacca atgtgacttt gtatctcggg ttgaaagacg atccacgcaa actaggattt
     6001 cggggcgaga actactggat ctattcatct tacgaccacg atcagagttt tgcacagcgg
     6061 agttcctgga tcgaatcggg caaacctccc ggtgcgtatc tttcttttcc atccttgaaa
     6121 aacagagaga gcaaaagcca cacggcggag atcatagcct ttacggacta tggtccgttc
     6181 gagaagtgga aggacgcctc ctggaaaaaa aggggggagg aatacgaggc catgaaagaa
     6241 aagatcgccc agtctttgat tgcttatgtg gatgaacgcc ttcctggttt gaaatcgatc
     6301 attgattttc atgaactatc tacgcccatt actaatgaac attttacagc ccatcctcgt
     6361 ggatcgatct acgggctggc ctgcgtgcca gatcgattta gagaagaaaa ggttgcattc
     6421 tgtcgggcaa aaactccaat cgaaggattg tttctgtctg gcgcggatgt ttcctctccc
     6481 gggattgccg gcgcgatgat gggtgggttt gcatgcctta cgcagattgt gagcgggttt
     6541 acaattttgt ctttgtttcg ctcaaggaaa tgagattcgc atacttgctg ttagttgcga
     6601 gtttcgcgct agtttctcct gtcgcaagtc gcgatttgca gaaccacgaa ttggagattc
     6661 gctacgggcc ggcgcaaaca tgcggaacat gcgctggaca gcatctgtgg cagcccatgg
     6721 caaaagcgga tgctttgttc cagggaacat tgaatgtttc cgatgactgg atcctgggcg
     6781 gttacggcaa ggcttctgtc tatgatcaca tcttccaggg agcaagtggc ttcttgcttg
     6841 gctggaatct tggtttggtg gaagcgcggg gttatgctgg aattgcctac gcgggtggag
     6901 tgacggagcg tgtgcgaaga ggattctttg tctatgatgg gcagactcgg gccacctacg
     6961 atttgggtgt gtcgcttcgc tacccggtgg cagatcgatg gcgcctaacg ttgaactatg
     7021 cgcacaattc caacggagaa gatataggaa tgaattttac cgggctgcga agaaagaatc
     7081 ctgggattga ttccatttct tttggtgtat cttacgtgct tggccagaca ccggcgctcc
     7141 ttctaccctg acgcgccttt cagaggtcgc gcgcaatgat ctcagtcaaa tcttctgctc
     7201 tgcgaggtgg ggagaaaaag aaaccctgcg ccatatcgca tttcatatcc gagagagtgt
     7261 tgagcgtcct ttcgtcttcc acaccttctg caatcacaat gaggccgagg gtatgagcca
     7321 ggcgaacggt tgaattcaca atgaccatgt ttctttcact ttccatcatc tgacgtacga
     7381 atgaaagatc gatcttgagc gcatcaatgg gcaggcgcga tagataagcg agcgaagagt
     7441 accccgttcc aaagtcatca atcgaaagat ccacacccag cgaatgcaaa cgattcaata
     7501 cgctcagagc tctctctgga tccgccatga ttgcgctttc agtgatttcg agctcaaagc
     7561 agcttctttc tactttatgt ttcttaagta tcgcgtctac tgtgagcggg tattgctcat
     7621 ccataaggtt tcgcacggat agattgactg cgatctttac cgtatgaccc gcgttcttcc
     7681 agactctcca ctgacgggac gcttcttcga atacccagtt tgtaagcgga atgatcaggt
     7741 ttccagtttc cgccagagga acgaattctc caggagaaac cattccgtgc actggatgat
     7801 gccatcgcac taaagcttct actccaacga gttttcgatc gtgcaaactg tatttgggct
     7861 gataatgaag agtgagctgg ctctcgcgaa tggcaagacc gagctctgtg agaagtgcaa
     7921 gtcttctgcg cgaataacgg tcctgatccg gggcatacat ggctacgcca acggacttct
     7981 tctttgccag atacatggca acatccgcac aacgcaaaag ggaaatagga tcctgaccat
     8041 ggaacggata tacagcgacg ccaatgctgc agtcaatttc caggttcatt ccctcgagca
     8101 ggaatggttc gcgcaaaagg tgatgcaatg tctgtgcaat gccttccgct tccgcgggtt
     8161 cgtctattct ggggagaaga attccgaatt cgtctccacc cagacgcgcc atctccgctc
     8221 caatggattt cagataggat tccatgcgcg ggccaatcct cttgagcaga aggtcacctg
     8281 tgaaatgccc cagtgtgtcg ttgatttctt tgaatcgatc cagatccatc aggagcaatg
     8341 ccagctgacg tccctctttt tctgcgcggc caattgcttc gagcgcgctt tcaatgagaa
     8401 attgcctgtt tggtagatct gtcagcgaat cgtgcgaagc ctggtgttcg agccttgcca
     8461 catccttttt tcgcgctgtg atatctgtgg ctacgagcaa ggccgctgta atggcccctt
     8521 catggcgaat cggaacaatc ctgcttgcgt agtgacgttc ttctccggga gaacccgctg
     8581 cggcttgttc atattcgatt tccaggccgc gctcaaatac gtctgcgagg cgtactggtg
     8641 ttagagtttg ttcttctggt ggcatgaaat cagcgatttg ctttccgatg atttcttcgc
     8701 gcttccaacc aggaaacaaa tggttgatga atagaatttt tccctgacga tctacgagta
     8761 gaatgaatgc gggaacgttc tcggcaatca gcttccattt ttcttccgag tcgcgcagcg
     8821 cgatttcagc cgcgagtcgc gctcccattt ctcgagcgat tcccagagtc aaagcgggtg
     8881 tgatcgggcg cgcattgatt tct
//
LOCUS       DAOQJL010000095         8748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4296_length_8748_cov_4.773381, whole genome shotgun sequence.
ACCESSION   DAOQJL010000095 DAOQJL010000000
VERSION     DAOQJL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_8748_cov_4.773381"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLG78_05995"
     CDS             <1..1380
                     /locus_tag="PLG78_05995"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE22695.1"
                     /translation="KALDHCTSRLNHSLEELKKLVRIPGVSFDGFDPSQVRDSASAAA
                     EHIHSSGMKNVEVIQFGDVHPYVFAEWMEHPDLPTVLLYAHHDVQPPGDEKGWNSKPF
                     DPVERGGRLFGRGTADDKVGILAHTAAISAYLNTAGRLPVNVKLLIEGEEEIGSSHLA
                     EFVRRHREKLRADCMILTDCSNIDTGIPSITTSLRGLVSVDVELRGLPQPVHSGFWGG
                     VVPDPAIALAQLLGKLTDEEGNILIPGILDDVRPLSVAEIKEFSTLDLEKIVRAGTQL
                     PDHLPLRGTSEELAKRMWREPSLSVLAFESGSRKMVANIIQDGAWARVSMRIVPDMKP
                     QKTQDILSDFLRSHCPRGFEITITPEAAADWWTVQDLNDPIFDIAARSLEKGYGQKTR
                     FVGCGASIPFVQPLSQELGGIPAVLLGVEDPYSNPHSDNESLHLDDFRKILISQVHML
                     GELARYRRG"
     gene            complement(1409..2074)
                     /locus_tag="PLG78_06000"
     CDS             complement(1409..2074)
                     /locus_tag="PLG78_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNE22696.1"
                     /translation="MNKTVTILMIAASVSCLTLVIYCIYGHLRITRVSQGVPIPNYET
                     EASALLIIDMQRDATEPSGRLVMNPAATDRIIENLNAVTPALRKRRTLFVMIRHEWEE
                     NWILQILTNHSLRPGTRGSEVDPRIAVKPDTIITKQTSDSFGTPALDAYLRENKISHL
                     YLSGTGANHCIRRTAEAALQRGYKVTVLRELVAGKTDAETIEGLQSMEKSGGKIASVE
                     SIR"
     gene            complement(2129..2725)
                     /locus_tag="PLG78_06005"
     CDS             complement(2129..2725)
                     /locus_tag="PLG78_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE22697.1"
                     /translation="MRPLSDSEQKRTREILERISAIPDSEWQHAKEMLITNDYPAGSF
                     LLRAGENPMQSFVLLEGAVRVYYVSRGGREYNRAFLAEGGIAASMRSVIAAEPSHLFI
                     QAMVPVKAVLLRRKFVMQLYARHECWNRLGRISAEAALIALESREALTFESLAERYRK
                     FLQDYAAFPYKIPNYQIASYLGITDVALSRLRKRMNLI"
     gene            complement(2980..3549)
                     /locus_tag="PLG78_06010"
     CDS             complement(2980..3549)
                     /locus_tag="PLG78_06010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE22698.1"
                     /translation="MKHTYAIMRHGQSQANVEGIIVSDPAIGVARYGLTEAGKKQALE
                     SARSSDFKARAIYSSDFRRARETAEIVAALQKAPVTETELLRERFFGDYNGTPDTQYQ
                     RVWADDSASIEHGRGVESPFSVLGRVLSLVETIESSILAPTPVLLVAHGDVCQILLAH
                     CAGLSPFLHRSIPHMQTAEIRRIRFPENP"
     gene            3637..4434
                     /locus_tag="PLG78_06015"
     CDS             3637..4434
                     /locus_tag="PLG78_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNE22699.1"
                     /translation="MIGPRRIVCLTEETTELLYLLGEEERIVGISAYTVRPPQARNEK
                     PIVSAFLNGSIPKIKALEPDLIIGFSDIQADLAKSLIAEGLNVLVTNQRSIEEIFETM
                     QMVGAIVGKGEDTARWIGSFRAKLDSVQVEGERRFRVRSAKPRVFFQEWDEPLITGIR
                     WVTELVEVAGGVDIFPEFRTAPMAKDRITTAKEVLARNPDVIIGSWCGKPMERTWIDQ
                     HLGELACVRAGNVHEIDSSIILQPGPALFLEGVDALARAIFNERLHQ"
     gene            complement(4503..5132)
                     /locus_tag="PLG78_06020"
     CDS             complement(4503..5132)
                     /locus_tag="PLG78_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22700.1"
                     /translation="MIDFNLGLCRIGPMAAKKSGKKSGKKAGKKAGKKAGKKKKRGRT
                     GPGSPLSVETEGGYSGVEDTVPTFSSTVSSEHTAGGEGKSSSPLLLVGLGGVVAVVLI
                     FFLFRGKNHDQSPAPSSQSAEISAPASVGTETTREYTTKQGQTLTSIAESELKDKTRA
                     KEILELNIQNIAKLKAMPENKGLEATNLKNLGVDKPFGANARKLLLPAK"
     gene            complement(5424..5645)
                     /locus_tag="PLG78_06025"
     CDS             complement(5424..5645)
                     /locus_tag="PLG78_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE22701.1"
                     /translation="MKFLGNKVRELREAREWTQIEMARRLWETGKFKGKTRQRVHQIE
                     SGEHASARTISALCEVFEVQPETFFDNEF"
     gene            complement(5698..6453)
                     /locus_tag="PLG78_06030"
     CDS             complement(5698..6453)
                     /locus_tag="PLG78_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22702.1"
                     /translation="MFYKASDLLPFIVQLAFGFALFFAEKTGKFQLSYSKFRKGGNMN
                     PRLAMFLIYFPAPIIYTVASMSGGLKNSPYHDALFYCFLFHFGKRCLEILFVHKYSRP
                     MGFGTVLLIGSLYGSIGWTAGTLHSLVSKEVGESSGVIPFLVIGGIIFAIGQAINLYH
                     HVLLAALRKEDSTAYIVPEGGLFSKVVCPHYLGEIMAWFGYAAMSGFLPMLGIAFIMS
                     LYLTGRSLRTVSWYKENVSGFPANRRALIPGIL"
     gene            6506..6967
                     /locus_tag="PLG78_06035"
     CDS             6506..6967
                     /locus_tag="PLG78_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNE22703.1"
                     /translation="MQYWLMKSEPDVFSIDHLAKLPGKTGSWEGVRNYQARNHLQAMK
                     KGDLALFYHSSADIIGVAGIVTIAGEAEPDTFALDKKSIYHDPKATREKNPWVFVRVK
                     YQKKFKEVVTLATLRQTAGLEDMLVLKKGMRLSIQPVTEKEFKIIEKMGSK"
     gene            6964..8127
                     /locus_tag="PLG78_06040"
     CDS             6964..8127
                     /locus_tag="PLG78_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22704.1"
                     /translation="MSKKYTLGLDRPAPGILERLGAKAFRSFASRRMLHRPTPSDDVL
                     VKELRVITWIGAFLAFAAGSLTTVPAVYLQVIHDGDPFAERMFWMFVPTIVMTGVEFA
                     LLFYTTVWCVVRVAEETGHGPDELDRPLFLGSDSLPNLLSRAALEIPDPVYQFLGIDP
                     TKLVPQKTLLIIGIIYKIKVMATNALVKLVLMRFLGNTVMRMSVAYVAVVITGIWNSI
                     VLWRVLRETRLRLSGHMLVRFLTTEIITPERIHSMPPLAREATVRAVANAVVLTQNYH
                     PNMLLLLVRLCEITGIDSPGDYDEWDRFVEILGQLEPEDREFQLDLLSIAAAFDGKLS
                     KFERKYLPHAFGPQTDWYMNRIRVLSRLMVQGRIRAAISHCFEDFNTDVTFTR"
     gene            complement(8138..>8748)
                     /locus_tag="PLG78_06045"
     CDS             complement(8138..>8748)
                     /locus_tag="PLG78_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22705.1"
                     /translation="FRNIQNLSQSTILCVNLLYENSDAWERMFPSGTTLSAVDFFPTG
                     FKILGFTSNWSSTGIPGLRQKKVDLIEVQVCRWQQFIGKDFRSIAAAVHAELKTALPD
                     LPEPTDFYINRWDTYTGYRPGDEKNRPGIQSPIENLMLIGDWVFVPQHSIFMERTNVC
                     AKWVTNLLLEKIGQKEGKIRILESGTPDWQLDLLGLVTSVRV"
BASE COUNT         2112 a         2094 c         2289 g         2253 t
ORIGIN      
        1 aaagccctcg atcattgcac ttccagattg aaccactccc tggaagaact caaaaagctg
       61 gttcgcattc ctggcgtcag ttttgacgga tttgatccat ctcaagtaag agacagtgcc
      121 agcgccgccg cagaacacat ccattcttct ggaatgaaga acgtggaagt gatccaattc
      181 ggcgacgtgc atccgtatgt gtttgcggaa tggatggaac atccagattt gccaacggtg
      241 ctgttgtacg cgcatcatga cgttcagcct ccgggagacg aaaaaggttg gaattctaaa
      301 ccattcgatc ctgtggaaag aggcgggaga cttttcgggc gaggcactgc ggatgataaa
      361 gtcgggatac ttgctcatac tgctgccata tctgcctatc tcaataccgc cggacgattg
      421 ccggtgaacg tgaaacttct gatcgaggga gaggaagaga tcggcagttc gcatcttgcg
      481 gagttcgtgc gcagacatcg tgagaaactc cgtgcagact gcatgatctt aaccgattgc
      541 agcaacatcg acactggcat tccgagcatc acgacttctt tgcgtggact ggtttccgtg
      601 gatgtggagc ttcgcggatt gccgcaaccg gttcactccg gtttctgggg tggcgtcgtt
      661 cctgatcctg ccattgctct tgcgcaactt ctaggaaagc tgacggatga agagggaaac
      721 attctgattc cgggtatcct ggatgacgtt cgtcctttgt ctgttgcgga aataaaagag
      781 ttctctacgc tggatctgga gaagatcgtg cgcgctggaa cgcagcttcc ggatcattta
      841 ccgttgagag gcaccagtga agaattggca aagcgcatgt ggagagagcc gagtctgagc
      901 gttcttgctt ttgaaagtgg ttcgcgcaag atggttgcca acatcattca ggatggagcc
      961 tgggcgcgcg tgtccatgcg aatcgtcccg gacatgaagc ctcaaaagac ccaggacatc
     1021 ctctctgatt ttctccgcag tcattgcccg cgtggttttg aaattaccat cactccagaa
     1081 gcggctgcag actggtggac agttcaggat ttaaacgatc cgatttttga cattgcggcg
     1141 cgttctcttg agaaagggta cgggcaaaag actcgtttcg tgggttgcgg agcaagcatt
     1201 ccgtttgtgc aaccgctttc gcaggagtta ggcggaattc ctgctgttct tctgggcgtt
     1261 gaggatccgt acagcaatcc gcactctgac aacgaaagcc tgcatctgga cgacttcaga
     1321 aaaattctga tcagtcaggt gcacatgctc ggcgagctgg cacggtatcg tcgcggctga
     1381 tcgggctttc gttgccaggg catcctgttc agcgaatcga ttcgacagag gctatttttc
     1441 cacctgactt ttccatgctt tgtagccctt ctatcgtttc tgcatccgtt ttgcccgcga
     1501 cgagctctct gagcactgta actttgtaac cgcgctgcag agctgcctct gccgtgcggc
     1561 gaatgcaatg attcgcaccg gtaccactca agtagagatg cgaaatcttg ttctctcgaa
     1621 gataggcgtc aagggccggt gttccgaagc tgtcgctggt ttgtttcgtg atgattgtgt
     1681 ccggcttcac cgcaattctt ggatctactt cgcttcctcg cgttcctggt cgtaaggaat
     1741 gattcgtaag tatttgaaga atccagtttt cctcccactc atgccgaatc atcacgaaga
     1801 gagtgcggcg tttgcgcaag gctggcgtaa cggcattgag gttttctatg atccgatctg
     1861 tcgcggccgg attcatgacc agccgcccag atggctcggt ggcatccctc tgcatgtcga
     1921 tgatcaggag cgcagatgct tctgtttcat agtttggaat cggcactccc tgagataccc
     1981 gagtaattct cagatgtccg taaatgcaat agatcactag cgtcaggcag ctaacagacg
     2041 cggcgatcat aagaatagtc actgttttgt tcatggccca agtatacccg ggtaagaact
     2101 ggtttgtcct taaccctggt taattctatc agatcagatt catgcgtttt ctcaatctcg
     2161 aaagggcgac gtccgtgatt ccaaggtagg aggcaatttg gtagttgggg atcttataag
     2221 gaaatgcagc gtagtcctgg aggaattttc gatagcgttc cgcgagagac tcgaacgtga
     2281 gtgcctctcg agattccagc gcaatcaagg ccgcctctgc actgattcgt ccgagccgat
     2341 tccagcactc gtgacgcgca tacagctgca ttacgaattt tcgtcgaagt aaaacagctt
     2401 tgaccgggac cattgcctgg ataaagagat gagaaggctc agccgcgatg acactgcgca
     2461 ttgaggctgc aattcctcct tccgcgagga acgcacgatt gtattcacgt ccaccgcggc
     2521 ttacgtagta cactcgcact gccccttcca ggagcacaaa tgattgcatc ggattctcac
     2581 ccgcgcgcag caagaagctg ccagccgggt aatcgtttgt tatgagcatt tctttcgcgt
     2641 gttgccattc agaatctgga atcgccgaga tgcgttccag aatttctcga gttcgttttt
     2701 gttctgaatc gctgagaggt cgcacggatc ctgatcgagt cccgttggcc aaagacaagt
     2761 agaaagcgag cccgattggg atcgcgccgc tcgcctggaa gcgtggtaag tccggcaaaa
     2821 cggccaggct aatgcgacat tcgccggatt atgtcacatt acgggccgca aaaatggttt
     2881 gacacggagg aaaaagccgc cctccgaaga gacataatcc gggcgaatta tgtctcataa
     2941 attatgctga atattgaatt tacttaaagt taatgaaatt tatggatttt ccgggaatcg
     3001 gattcgtctg atttctgcgg tttgcatgtg cggaatgctg cgatgcaaga aaggagaaag
     3061 tccggcgcaa tgtgccagaa gaatctggca aacgtctcca tgtgccacga gaagtaccgg
     3121 tgtgggtgca aggatagacg actctatggt ctcaaccaga gatagaactc ttcccagcac
     3181 ggagaaagga gattccactc cgcggccgtg ttcgatggaa gcgctatcat ccgcccagac
     3241 tcgctggtac tgtgtgtctg gtgttccatt gtaatcgcca aagaaacgct cgcgaagcaa
     3301 ttctgtttca gtcactggcg ccttctgcaa agccgcgacg atctctgcgg tctccctggc
     3361 tcttctgaag tcggaagaat agattgcccg ggctttgaaa tcagaagagc gagcgctttc
     3421 gagagcttgc ttctttccgg cttctgtgag gccgtagcgt gcaactccaa tcgcaggatc
     3481 ggacacgatg attccttcta cgttagcctg gctctggccg tgtcgcatga ttgcataggt
     3541 gtgtttcatc ttgcccttcc aactttatgc gaccaggcca aatcggcaca tcctaaaatg
     3601 gattgcacgg agtggatctg tgttcgatgt tacggcgtga ttggcccgcg taggatcgtt
     3661 tgtctaacag aagaaacaac cgagcttctg tatttgctgg gggaagagga gcgcatcgtt
     3721 ggaatttccg cctacacagt gcggccgcct caagcacgaa atgaaaagcc cattgttagc
     3781 gcgtttttga atggcagcat tcctaagatc aaggcacttg agcctgattt gatcatcggt
     3841 ttctctgata ttcaggcgga tctcgctaaa agtctcatcg cagaaggatt gaatgtgctc
     3901 gtgaccaatc agagatccat tgaagagatc tttgagacca tgcagatggt gggtgcgatc
     3961 gttgggaaag gagaagatac cgctcgttgg attggctcgt ttcgtgcgaa actggattca
     4021 gtgcaggttg agggcgagag gagatttcgt gtgcgatcgg caaagccccg tgtatttttt
     4081 caagaatggg acgagcccct catcacaggg attcgctggg tgaccgagct cgtggaggtg
     4141 gcgggcggcg tggacatttt tccggaattt cgcacggcac ccatggcaaa agatcgcatt
     4201 acgaccgcga aggaggtcct ggcccgaaat ccggacgtga tcattggttc gtggtgtgga
     4261 aagccaatgg aaagaacctg gatcgaccag cacctgggag aacttgcctg tgttcgagcg
     4321 ggcaacgtcc atgaaattga ttcatcgatc atactgcagc cgggtccggc tctgttttta
     4381 gaaggagtgg atgcactcgc acgcgcgatc ttcaacgaga gattacacca gtagaacgaa
     4441 gcgcgtcagg gatgggtaaa aaaaagccgt agattcagac cgaatctggc tgaacctacg
     4501 gattattttg cgggaagaag aagtttgcga gcgtttgccc caaacggctt atccactccg
     4561 agatttttca gatttgtcgc ttcgagaccc ttgttttcag gcatcgcttt gagcttcgca
     4621 atattctgaa tgttcaattc aagaatttcc ttggcacgcg tcttatcttt gagctcggat
     4681 tcggctattg aggtcagtgt ttgaccttgt ttagtcgtgt attctcgtgt agtctctgtt
     4741 cctacagaag caggcgcgct gatttcagca ctctgagacg atggagcagg agattgatca
     4801 tgattctttc ctctgaagag gaagaagatg agaaccacgg cgacaacgcc gccgaggccc
     4861 acgagaagaa ggggggaaga agacttccct tctccccccg ctgtgtgttc agaactgaca
     4921 gtagatgaaa aggtggggac cgtatcttct acgccagagt accccccttc ggtttcaacc
     4981 gaaaggggcg agccggggcc tgttcggcct cgctttttct ttttgccggc tttcttgcct
     5041 gcttttttac cggctttttt gccggatttc ttaccagatt tttttgctgc catgggccca
     5101 atacgacata atcctaaatt gaaatcaatc atttttttat actattgggt tctaaagtga
     5161 aaaaaatttc aaaatttgtg ttttgaatca tcttcgcacg catttcatcg cgaaatttca
     5221 ttcatcaagt gcgcattgcg cgttcgaatg cgattatcat cgcatgaaaa atgatttttg
     5281 atgattcaat gtgcattttc gaggcatgca cgttgcgatt ttcagtaaat gaatgatgtt
     5341 gaagtgatgc tgaaacgaat tttggaatga aaagtgcgcg tagcgagcat caaatgatgc
     5401 aatcacatcc gcaaaatcaa aaatcagaac tcattatcga agaaagtttc tggttgaact
     5461 tcaaaaactt cacatagagc actgattgtg cgcgcagatg catgctctcc ggattcaatt
     5521 tgatgaactc tctgtctcgt tttgcctttg aatttgcccg tctcccatag tcgacgggcc
     5581 atttctatct gtgtccactc tctggcttca cgcaattcgc ggactttgtt gcccaggaac
     5641 ttcatttcac agtcataaga ctgcaaaaat ggcatgtcaa acgttttgca cgaatgatta
     5701 aaggatgcct ggtatcaatg cgcgcctgtt agccgggaat ccggatacgt tctctttata
     5761 ccagcttacg gttcgaagtg atctgcccgt aagatacaga gacatgatga atgcaattcc
     5821 aagcatggga aggaagccag acatggctgc gtaaccaaac catgccatga tctcacccag
     5881 atagtgcgga cagactactt ttgagaataa tccaccctct ggtacaatgt atgctgtact
     5941 gtcttctttg cgaagggccg cgagtagaac atgatgatac agattgattg cctggccaat
     6001 agcaaagatg attccaccga tcactagaaa aggaatcact ccgctggatt ctcctacttc
     6061 tttggagacc agagaatgca gagtccccgc agtccatccg atcgatccat acaatgaacc
     6121 aatcagcaat acagttccaa atcccatggg tcgcgaatac ttgtgaacga acagaatctc
     6181 aaggcatctc ttgccaaaat gaaatagaaa acaatagaac aaagcatcat gatagggcga
     6241 gttcttcagt ccgccggaca tacttgccac ggtgtaaatg ataggggctg gaaagtagat
     6301 caggaacatg gccagcctgg gattcatgtt tccgcccttg cgaaacttgc tgtaagatag
     6361 ctggaatttg cccgtttttt ccgcaaaaaa tagcgcaaaa ccgaatgcga gctgaacgat
     6421 aaatggtagt agatccgatg ccttataaaa cacgattcca gcgggccctc gtgaacggaa
     6481 gggtcaatag aatttggagt ggatcatgca atactggctt atgaaatccg aaccggacgt
     6541 cttttccatc gatcaccttg cgaaactacc aggcaagact ggaagctggg aaggggtcag
     6601 gaattaccag gcgcgcaatc atctgcaggc aatgaagaag ggcgatcttg cactcttcta
     6661 ccattccagt gcggacatca taggcgtggc cgggattgtg acgatcgcag gagaagcgga
     6721 gcctgataca ttcgctctgg acaagaaatc gatctaccat gatccaaaag caacccggga
     6781 aaagaatcca tgggtatttg ttcgcgtaaa gtatcagaag aagttcaagg aagtagtgac
     6841 tctggcaact ctcaggcaaa cagcgggcct tgaggatatg ctcgttctaa agaaaggaat
     6901 gcgtctctcc atccagccag tgactgaaaa ggaattcaaa atcatagaaa agatgggtag
     6961 caagtgagta agaagtatac actcgggctg gatcgaccgg cccccgggat cctggaacgc
     7021 ctcggggcaa aagcgtttcg atcgtttgca tcgaggcgca tgctccatcg tcctacacct
     7081 tctgatgatg ttctggtcaa ggaactgagg gtgatcacat ggatcggcgc cttccttgcg
     7141 ttcgctgccg gctcccttac cacagtcccg gccgtctatc ttcaggtgat tcatgatggc
     7201 gatccgttcg cagaaagaat gttctggatg tttgttccga cgatcgtcat gacaggggtg
     7261 gagtttgctc ttttgttcta tacaactgtc tggtgcgtgg tgcgagttgc ggaagaaacg
     7321 ggccatggac cggacgaact cgatcgtccg ctgtttctgg gaagtgattc attgccgaat
     7381 ctactttccc gcgcggctct tgaaattccg gatccagtgt atcagtttct tggaattgat
     7441 ccgaccaagc tcgtgccaca gaaaacactt ttgatcattg ggatcatcta caagatcaaa
     7501 gtgatggcaa ccaatgcact tgtgaagctc gtgctgatgc gttttctggg caacactgtg
     7561 atgcgaatga gcgtagctta cgttgcagtc gtcatcacag gtatttggaa ttctatagta
     7621 ttatggagag tgcttcgaga gactcgtctt cgtctatcgg gccacatgct ggttcgattt
     7681 ttgactactg aaatcatcac ccccgaacgc atccattcca tgcctcccct ggcccgcgaa
     7741 gctaccgtcc gcgcagtggc caatgcggtg gttttgaccc agaattatca ccccaatatg
     7801 ctccttttgc tggttcggct gtgcgaaatc acgggaatag acagccccgg tgactatgac
     7861 gaatgggacc gatttgtgga gatcctgggg cagctggaac cggaagatcg cgagtttcag
     7921 ctggatttgt tgtccattgc cgctgctttt gatgggaaat tgtccaaatt cgaaaggaag
     7981 tacctgcctc acgcgtttgg ccctcaaaca gactggtata tgaatagaat tcgtgtcttg
     8041 agtcgtttga tggtccaggg gcggatccgt gctgccatct cccattgctt tgaggatttc
     8101 aacacggatg taaccttcac ccgttgaagg ttcgcgatca gactcggacg cttgtgacca
     8161 ggcccagaag atccagttgc cagtctggcg tccctgattc caggattcgg atctttcctt
     8221 ctttttgacc aatcttctct aatagaagat tggttaccca tttcgcacaa acgttggttc
     8281 tttccataaa gatggaatgc tggggaacaa acacccagtc tccgatcagc atcaggtttt
     8341 cgatggggct ctggattcca gggcgattct tctcatctcc tggtctgtag cctgtgtagg
     8401 tgtcccagcg gttaatatag aagtctgttg gttccggcag atcggggagg gccgttttga
     8461 gctctgcatg tacggcagcc gcaatggatc gaaagtcttt tccaatgaat tgctgccagc
     8521 ggcatacctg gacctcgatt agatccactt tcttctgtct gagccctgga attccggtgc
     8581 tcgaccagtt gctcgtgaat ccaaggatct tgaagccggt agggaagaaa tccacagcac
     8641 tcagagtggt gccagacggg aacattctct cccaggcgtc agagttctca tagagaagat
     8701 ttacgcacag aatcgtagac tggctcagat tctggatgtt tctaaaat
//
LOCUS       DAOQJL010000096         8738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4307_length_8738_cov_4.617644, whole genome shotgun sequence.
ACCESSION   DAOQJL010000096 DAOQJL010000000
VERSION     DAOQJL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4307_length_8738_cov_4.617644"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PLG78_06050"
     CDS             <1..1392
                     /locus_tag="PLG78_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE22706.1"
                     /translation="YMKTIRCRFILVSIILLPLLLGVLPVTGNAPECLTLNEAMALAQ
                     EKSPDMRKARAALLLARGEFVQKDRLIAANPEISVSGTRGTRATSPDVSVSPSAALKG
                     EQPVDVSPSILSGPQQQRINGYEVELSQELDLAGKSSIQKKIARVEIEKADAQLLLTR
                     LQVRAEVRTQLTTFLMSEALAADLERQLEHLRLIKQRMGPGFTDQRLGTYVGVVFQND
                     LARADIELRNLLLTRTNAKQTLDQMLGVTTVKVCTLPPTSSRITNPTLPEESELIAGA
                     HANSPRLRLVSLNSQQSELGSNLADIAMIPDPTFFLGAGEQSIGSSKLAPSIAGPSSE
                     REKTFRFGVRIPIPVFDRKQGSADIARAHRESTGADREAAERNLESSIAVLRNRYIAL
                     MEIVKQLAKLQQPFASLESLDGPFLSGRIPYSDWSEQYRRVAEIKKQYLDTRAEAARV
                     LGEIEIIIGREIQ"
     gene            1831..2160
                     /locus_tag="PLG78_06055"
     CDS             1831..2160
                     /locus_tag="PLG78_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22707.1"
                     /translation="MLRKLIMTAAILAFITPIMAEGRGWLRGEQVLDSSSDVQVMNVS
                     EVQPKVGEEFDVYRIALETNPAQRGMRHRYKKIGRVRVAAIVDPHVAKATVIWGLSGD
                     SYLIETK"
     gene            2222..3067
                     /locus_tag="PLG78_06060"
     CDS             2222..3067
                     /locus_tag="PLG78_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22708.1"
                     /translation="MKGNKKLLLGVAAILTIGVGVWFLLTRSKSPDMADMDHGADHSD
                     YMLMLNTGTKYKVSQPNTLKFMVHDKEGKVVRDFEIMHTKIMHFIVVRKDLANFQHLH
                     PDFDKESGVFSLPAIKFETDGEYRLFADFTPSGGMAQGHEHHAGMAMGQTLGVSVVAG
                     NESKYRPEPIGDMRTTDRRENYLFNLENTGSGLERALRFTISENGKPAKDLQPYLGAL
                     GHIVVLSEGDLLYVHAHPDESAANAPGAVSFEVHFPRAGKYKIFGQFQHRGRVLTASF
                     VTEVK"
     gene            3093..5429
                     /locus_tag="PLG78_06065"
     CDS             3093..5429
                     /locus_tag="PLG78_06065"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE22709.1"
                     /translation="MNEHSHTHHHKHEHNTPQKGENATLDPVCGMSVKQDSRHRATYN
                     DKEYLFCSAGCLTKFTENPEHYASPSKRQTHPIAENANTVSNSAGDYTCPMHPEVRQK
                     GPGSCPKCGMALEPVSGGNEDLTEYKDMRRRFVVSAILSAPVLFLAMGEHLPMLRDIP
                     HHFSMWVQFVLASPAVLWGGWPFFARAWRSIVTLQLNMFTLIAIGVGAAYFFSVVALL
                     APGLLPQGENVFVYFEAAAVITALALLGQVLELRARTQTGTAIKLLMGQAAKTARIIR
                     DGQEFEVPIENVAAGNLIRVRPGEKIPVDGVIIEGGTTIDESMLTGEPMPQEKKADDR
                     ITGGTLNQTGSFVMKATGVGSDTVLSRIIHMVQEAQRSRAPIQKLADTVSGYFVPAVL
                     VIAVLTFISWMIWGPSPNLSYAFMNAVAVLIIACPCALGLATPISIMVAVGAGARVGV
                     LIKNAESLEKLEHIDTLVVDKTGTLTQGKPRVTEVVVGAGYEEDQLLSLVASLETASE
                     HPLSRAVIDFAKKKNLNIVSPENVQSITGGGISGTVSGHEVIVGKEGLLRARQISGFE
                     NAISTKNQASTLIYAGIDGKLAGVLGLSDPIKQSTPEALQGLRNLGLNVIMMTGDNER
                     TGAAVAAELGIKEFYAGVEPQGKHDRLLAIKKKEHSVAMTGDGINDSPALAAADVGIA
                     MGNGSDIAIESAGVTLVKGDLMGIVRAIKLSKGAMRNIRQNLFFAFVYNALGIPVAAG
                     ILYLFGGPLLNPMIASLAMSLSSVSVIVNALRLRALRL"
     gene            5801..6064
                     /locus_tag="PLG78_06070"
     CDS             5801..6064
                     /locus_tag="PLG78_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22710.1"
                     /translation="MMFEICDLGWFPYYYKNVFDVFEGVVYKQDSDGRILCVRHNLKR
                     KLKEIAVEWDDRLEELEYITLSGHSPVANFFPPPFFMTQRPAP"
     gene            complement(6655..8214)
                     /locus_tag="PLG78_06075"
     CDS             complement(6655..8214)
                     /locus_tag="PLG78_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNE22711.1"
                     /translation="MAGMRTLFLLALVSAAFSLHAKPYIVSSVPNPRSQDGGFVSDPD
                     GILLAADRAELNGILRQIEQDTTAEYAVVILESIGTQIPKSFAVDLFNTWGIGKSGKD
                     NGLLLLVVLDQRRWEFETGYGLEGVLPDVVLSRLARDTFPDYFRKKQYGLAIIDVSRQ
                     IESILKHAAEEIGLSPEQRAEQLAREEEKLFAEYIQKTQTSRMWFGLGAFAFLCVYCA
                     VALFVRNNRKRPPSGQRGTAADKLILAPLSLWFWLPAVLIWPFMLVVVAYWNFYTIAF
                     IYQVSSFADSYLGYILGAVSYCMIGLMLIVQRQRRTTLLLKENPDPGESYRLIQEYLK
                     DAVPGLVFFPLFYGPYWIASKLRLGLLRRTPRKCLACNNVMRRLGESEEDPKLEPGQL
                     LEETLGSVDYDVWMCPQDSEIKIEKYILSSPYSECEACKFVTSVVIRNWTIQSPTYES
                     AGQGGEEQACRHCGRKKETRYVIAKLVRSGSASGGSYSSGGSSSRSSGSSSGGSFGGG
                     RSGGGGAGGSW"
     gene            complement(8223..>8738)
                     /locus_tag="PLG78_06080"
     CDS             complement(8223..>8738)
                     /locus_tag="PLG78_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNE22712.1"
                     /translation="INFVCQYRIVDPEKIAQIKSYEEQLYIILQLILREYVGTLTLDD
                     LLAKKEEINQFVLARLREKAAVLGVEFASAGVKDIVLPGDFKEIMNLVLIAEKKAQAN
                     LITRREEIASTRSLLNTARLMDENPTLYRLKELEFVERISDKVASISLSSGAGLLEQL
                     TGMIATRKKEP"
BASE COUNT         2211 a         2177 c         2254 g         2096 t
ORIGIN      
        1 tatatgaaaa ctattcgctg tcgattcatt cttgtaagta tcattctact tccgctgctt
       61 ttgggtgttc ttcctgtgac aggaaatgcg ccggagtgtc tcacactgaa cgaggccatg
      121 gcgctcgcgc aggaaaaaag tcctgatatg agaaaagcgc gcgcggcgtt gttgctagcc
      181 aggggagaat tcgttcagaa agaccgtttg attgcagcga atcctgagat ttctgtgagt
      241 ggcacacgcg gtacgcgcgc aacttcgccg gacgtttctg tttctccgtc agcggctctt
      301 aagggtgagc aacccgttga tgtttccccc agcattttgt cgggaccaca gcaacaacgg
      361 atcaacggat atgaagttga attgagtcaa gaactggacc tcgccggaaa gagttccatt
      421 caaaagaaaa ttgcgcgagt tgagatcgag aaggctgatg cacaactcct ccttacacga
      481 ttgcaagtgc gtgcggaagt tagaacgcaa cttacaactt tccttatgtc tgaggccctg
      541 gcagcggatt tagaacggca gctcgaacat ttgcgtttga tcaagcaaag aatggggcca
      601 gggtttactg atcagagact tggaacctat gtcggtgtcg tgtttcaaaa tgatcttgcc
      661 agagcggata ttgaattacg caacctcctg ctaacccgaa cgaacgcgaa acaaactctg
      721 gatcaaatgc ttggcgtgac gacagtgaag gtctgcacac ttcccccaac atcctctaga
      781 atcacgaacc cgacgctgcc ggaagaaagt gaactaatcg cgggggctca cgcgaacagt
      841 ccccggcttc gccttgtcag tttaaattcg caacaaagtg aacttggcag caacctcgcc
      901 gacatagcca tgatacctga cccgacattt tttctcggtg ctggcgaaca atcgattgga
      961 tcgagtaagc tggctccctc catcgccgga ccttcaagcg aaagggaaaa gacatttcgt
     1021 tttggagtga gaattccgat ccccgtcttt gatcgcaagc aaggatcggc ggacatcgca
     1081 cgcgcgcatc gggaatctac tggtgcagac cgcgaggccg ccgagcgcaa tctcgaatcg
     1141 tccattgctg tccttcgcaa tcgctacatc gcgctcatgg agatcgtgaa gcaacttgct
     1201 aaactgcaac agccgttcgc ttcgcttgag tctctggacg gtccctttct ttcgggcagg
     1261 ataccgtact ccgattggtc tgaacaatat cgcagagtag cggagatcaa gaaacaatat
     1321 ctggatactc gtgcggaggc cgcgcgcgtc ctgggagaaa tcgagatcat catcgggcgg
     1381 gagatacagt gagatcgcat tcgcttccgc tctggagggt ctcttcctcg gcaatccttg
     1441 ccgagatgct ctggctgttt acaattccat tgtttggagt gcatgttcat tcaaccgatt
     1501 tgcggcttga tcccgtaatt cacgcgcacc ataaaaacat ctctcatgac gccggcgcgc
     1561 cagagattcc taaaaatcaa atcccgactt ctccctcaga tcttcttttt tccggcaaac
     1621 tgaaaaagga tgacccaagc gggagacgcg tttgggattt gtcccacgcg atcattccat
     1681 ccgggtcatg gatgtcgcct aacgctactt gtttagctca tttgccagcg ttcattccat
     1741 tcgttaagtc ctggtttgcc acgcgaatca gaagtatccg cgctcctcca aacttgagcg
     1801 caaagtctaa acaaaaaatg gaggaattat atgttaagaa aactcattat gacggccgca
     1861 atactggcgt ttattacgcc aattatggcc gagggcagag gttggctcag aggggaacag
     1921 gtcttggact cttcatcgga tgttcaggtc atgaatgtgt ccgaggttca acctaaagtc
     1981 ggagaggaat ttgatgtgta tcggatcgct ctggaaacga atccggctca gcgcggcatg
     2041 cgtcatcggt acaagaaaat tgggcgagtg cgtgttgccg cgattgttga tccacatgtt
     2101 gcgaaagcga ctgtgatctg gggattgtcc ggcgactcgt atttgattga aacgaagtaa
     2161 acatcgaaat caggggagca atcccctgat gtttttttag aaaatattga ggagattaga
     2221 gatgaaagga aacaagaaat tactactggg agttgccgca attttaacaa ttggtgttgg
     2281 tgtttggttt ctgctaactc gcagcaagag tcccgacatg gcagatatgg atcacggagc
     2341 agatcattct gactacatgc tgatgctgaa cacaggtaca aaatacaaag tgagccagcc
     2401 caatacgctg aaatttatgg ttcatgataa ggaaggaaaa gtcgtccggg attttgagat
     2461 catgcacacc aagatcatgc acttcattgt tgtgcggaag gatcttgcga attttcagca
     2521 tctgcatccg gatttcgata aagagtcggg tgtatttagt cttccagcaa taaagtttga
     2581 aactgatggc gaatatcgcc tcttcgccga tttcactccg tcaggcggaa tggcacaggg
     2641 acacgaacac catgctggaa tggcaatggg ccagacactg ggtgtttccg tcgttgctgg
     2701 aaacgaaagt aaatatcgac ctgagccgat tggggacatg cgcacaaccg accggcgcga
     2761 gaattatctt ttcaatttgg aaaacacagg atcgggactt gagcgagctt tacgctttac
     2821 catcagtgag aatggcaaac ccgccaaaga tttacagcct tacctcggtg ctcttggtca
     2881 tatagtcgtt ctcagtgaag gcgatctttt gtatgtgcac gctcatccgg atgaaagtgc
     2941 agcgaatgca ccgggggctg tgagttttga agtccacttt ccgcgcgctg gaaaatacaa
     3001 gatctttggg caatttcaac acagaggaag agttctcacc gccagctttg taacggaagt
     3061 caagtgaggg atttgaccaa gaggaaacga agatgaatga acattcacac acgcatcacc
     3121 acaaacacga acataataca ccacagaagg gcgagaatgc tacactcgat cctgtttgtg
     3181 gtatgtccgt aaaacaagat tcgcggcatc gggcaactta caacgacaag gagtatctgt
     3241 tttgcagcgc gggttgtctt accaaattca cggaaaaccc ggaacactac gcctcgccat
     3301 caaagagaca gactcatcca attgcagaga atgcgaatac ggtatcgaac tctgcgggtg
     3361 actatacatg tccgatgcat ccagaggtcc ggcagaaggg accaggctct tgtccaaaat
     3421 gtgggatggc tcttgagcct gtctccggcg gcaatgagga tctaacggag tacaaagata
     3481 tgcgtcgcag attcgtggtc tcggcaattc tttcggctcc tgttttgttt ctggcaatgg
     3541 gtgaacatct cccgatgctc cgagatattc cgcaccattt ctccatgtgg gtacagtttg
     3601 tactcgcatc ccccgccgtg ctctggggtg gctggccctt ctttgcgcga gcctggcgct
     3661 ctatcgtcac gctgcaactg aacatgttca cgctgatcgc tatcggagtt ggcgcagcgt
     3721 atttcttcag cgttgtagca ctgcttgccc ctggacttct tccccaaggt gagaatgttt
     3781 ttgtctattt cgaggctgcg gctgtaatta ctgcacttgc gttgctcggc caggttctgg
     3841 agctgcgtgc gcgaacgcag actggcacag caatcaaatt gctcatggga caagctgcga
     3901 aaacggcgcg gatcattcgc gacggtcagg aatttgaggt tccgattgaa aatgttgcag
     3961 ccgggaatct cattcgtgtt cgacccggcg agaaaatacc cgtggatgga gtgatcattg
     4021 agggtgggac gaccattgat gaatcaatgc taacaggcga acccatgccg caggaaaaga
     4081 aggccgatga tcgcataacg ggtggaacac tcaatcaaac cggtagtttc gttatgaagg
     4141 caaccggtgt tgggagcgac acggtcctct cacgcattat ccacatggtt caggaagcac
     4201 aacgcagtcg ggctccgatt caaaaactgg ccgacaccgt gtccggatat tttgtgcccg
     4261 ccgttttagt cattgcggtc ttgaccttta tttcctggat gatctggggt ccgtcaccga
     4321 atctgtctta cgcgttcatg aatgcagtgg cagttctgat cattgcgtgt ccctgtgctc
     4381 taggtcttgc cacaccaatt tcgatcatgg tcgcggtcgg cgctggagcg cgagttggtg
     4441 tgcttatcaa gaatgctgaa tcactggaga agttggaaca cattgacacc ctcgttgtgg
     4501 ataagaccgg tactctaact cagggtaagc ctcgtgttac ggaagttgtc gtaggagcgg
     4561 gctatgagga ggatcaactc ctctctcttg ttgcttctct cgaaaccgcg agcgagcacc
     4621 ctctttcgcg cgcggtaatt gattttgcca aaaagaagaa tctcaacatc gtatcgccag
     4681 agaatgtgca atcgatcact gggggtggaa tcagcggaac cgtgagcgga cacgaggtca
     4741 tcgtcggcaa agaaggattg ttgcgcgctc gtcaaatcag tggatttgaa aacgcgatct
     4801 caacaaagaa ccaagcgagc acgctaattt atgctggaat cgacggtaag ctcgccggtg
     4861 ttctaggttt gagtgacccg atcaaacaat ccacaccaga agctctccaa gggcttagga
     4921 accttggctt gaatgtgatc atgatgacgg gagataatga acgaacaggc gcagctgttg
     4981 cggcggaact gggcatcaag gagttttacg cgggagttga accacaggga aaacatgatc
     5041 gattgcttgc gataaagaag aaagagcatt cagtcgcaat gacgggtgac ggtatcaatg
     5101 attcaccagc ccttgcagca gctgatgtgg gaatagcaat gggtaacgga tctgacattg
     5161 caatcgaaag tgcaggtgtt acgctcgtca aaggtgatct gatgggaatc gtcagggcga
     5221 tcaaactcag caagggagcg atgcgcaata tccgacaaaa tctattcttc gcgtttgttt
     5281 acaatgcact tggaattccg gtggcggcgg gaatcctata tctattcggt gggccgcttc
     5341 tcaatccaat gattgcaagt ctcgcaatga gtttgagttc agtgtccgtg attgtgaatg
     5401 cgctgcgctt gcgggcgctc aggttgtaat gggaaaccaa cttattgggc gaggggatta
     5461 ctcaatcaga agcagtgcgg cggatgatcg aagctggctt gaagaaattt gagaaaggtt
     5521 aagttttttt gcgatcattg cagaatctct tgacctgaat ataataccat agtattatat
     5581 tgcaaacaaa ataaaggggg ctgccatgaa agccatagaa aaaataattg atcacttcgg
     5641 ggggatggag gcgctcttgg agtccagaat catccgggtt aacattgatc cgtttcgttc
     5701 tcttttagtt agaattgcac aagccagccc aaacggtttg cctgcaatct caatcactca
     5761 ggaatgccgt gaaaacggtc caccccgagc ggaaattcaa atgatgtttg aaatctgtga
     5821 cctgggctgg tttccgtact attacaaaaa tgtctttgat gtttttgaag gtgtagtata
     5881 caaacaggat tcggatgggc ggattctctg tgttcgccac aacctcaaac gcaagctcaa
     5941 agagatcgcc gtggaatggg atgaccgcct tgaagaattg gaatacataa cgttgtctgg
     6001 acattcaccc gtcgcaaact tcttcccgcc gcctttcttc atgacgcaac gtccggctcc
     6061 atagccgcgc gcgccgcgca tcagctacgg tttcgaaccg tagctgatgc gaaaaggatc
     6121 tacatacaaa acgatcgaat caagaaacaa gcaaccacag cctgaaatcc atcctgagtt
     6181 cttccataat actatcaatc ttatccatct cttcaaggtt cgaactcaat cccgccctct
     6241 ccgaaatttt cataatacaa aagaatatca tgggcgggtg agaacccgcg aaactctcac
     6301 gagcaactca actcccacca caccacaacc attcaccgca aacgcgggtt cgtttctgtg
     6361 tgcgacgcaa gtcgcacctc atatgcacgc gaagcgtgcc tcctctctga acgtcgagcg
     6421 aagcgagaca accccgtcgc agattgcggc atgatcgcca cttcacgacg cgttccctcc
     6481 atggaccgag tcgtgaaggc tcgcacctcc tgtgctcgct gtgacgacca taccgcaact
     6541 gcgctccagt gggaattcgt gtaatattta atgaaattta ggaaatacgg ctttgaactg
     6601 tcacgccaga agagcattgc cgcatccacc gcaaacgcgg gtactcggga agagtcacca
     6661 gctcccgcct gctcctccgc ctccggatcg tccaccacca aacgagcccc ccgaagaact
     6721 gcccgagctt cgagagcttg agccgcccga cgagtaggat cctccgctgg cagaaccgga
     6781 gcgaacaagt ttcgcgatca cataccgggt ttctttcttg cggccgcagt gtctacaggc
     6841 ttgctcttca ccgccctgac cggcactttc gtaagttggc gattggatgg tccagttgcg
     6901 aatgacaacc gaggtgacga atttgcaggc ttcgcactca gagtaaggcg aagacaggat
     6961 gtatttttca atcttgattt ccgaatcttg cggacacatc caaacatcgt aatccaccga
     7021 accaagagtc tcttccagca gctgccctgg ttcaagcttc ggatcttctt ctgactctcc
     7081 gagacgccgc ataacgttat tgcacgcgag gcacttgcgt ggtgtgcgac gcagcaagcc
     7141 aaggcgcagc tttgaagcga tccagtaggg tccatagaag agaggaaaga agacgagacc
     7201 cgggactgcg tctttcaagt attcctgaat gagccggtaa ctttcaccgg gatctgggtt
     7261 ttctttgagc agaagcgtag ttctccgctg cctttgcaca atcaacatga gtccgatcat
     7321 gcagtaggat acggcgccaa gtatgtaacc caggtacgag tcagcgaagc ttgagacttg
     7381 ataaatgaat gcgatggtgt agaaattcca gtatgccaca accacaagca tgaaaggcca
     7441 gataaggact gcggggagcc agaaccacag agagagcggt gcgagaatga gtttatcagc
     7501 ggctgtccct cgctgaccgg aaggggggcg cttccgattg tttcgcacaa acaacgcaac
     7561 tgcgcagtat acgcacagga atgcaaatgc gcccagacca aaccacatgc gcgaggtttg
     7621 tgttttctga atgtattccg cgaaaagctt ttcctcttcc ctggccaact gctctgcgcg
     7681 ttgttcaggg gagagaccga tctcttctgc ggcatgcttc aagatgcttt cgatctgcct
     7741 cgatacgtcg atgatcgcaa gaccatattg ctttttacga aagtagtcgg gaaacgtgtc
     7801 tcgcgcaagg cgcgatagca caacgtcggg aagcacgccc tcgagaccgt agcccgtttc
     7861 aaactcccag cggcgctgat cgagtacaac aagaagcaat agtccgttgt ctttgccgct
     7921 ttttccaatg ccccaggtgt tgaacagatc gacggcgaaa gacttcggaa tttgcgttcc
     7981 gatggattca aggattacga ccgcgtattc cgccgttgtg tcctgctcaa tctgtctgag
     8041 aattccgttc agttccgcgc ggtcggcggc caaaagaatt ccgtctggat cagagacaaa
     8101 tccgccgtcc tgagatctcg ggtttggcac actggaaact atgtacggtt tggcgtgaag
     8161 tgagaaagct gcggaaacga gggcgagcag aaagagcgtg cgcatgccgg ccatagttgc
     8221 tctcatggct ctttctttcg cgtcgcaatc attcccgtta gctgctcaag caaacccgca
     8281 cccgacgata gggagatact ggcgaccttg tctgagattc gctcaacgaa ttcgagctcc
     8341 ttgagccgat acagtgtcgg gttctcgtcc atgagcctgg cagtgttcag taaactgcgc
     8401 gtggatgcaa tttcctcgcg ccgcgtaatt agattcgcct gagccttctt ctccgcaatc
     8461 agaaccagat tcatgatttc tttgaaatca cccggcaaaa caatgtcctt tacacccgca
     8521 ctcgcaaatt caacaccgag aacagccgct ttttcccgga gccttgccag aacaaactga
     8581 ttgatttcct ctttcttcgc aagcaggtca tcgagcgtca gcgtgcctac atactctcgg
     8641 agaatcaact gtagaatgat gtagagctgc tcctcgtacg acttgatctg tgcaatcttt
     8701 tctggatcaa cgattcggta ctggcataca aagttgat
//
LOCUS       DAOQJL010000097         8732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4312_length_8732_cov_4.433329, whole genome shotgun sequence.
ACCESSION   DAOQJL010000097 DAOQJL010000000
VERSION     DAOQJL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4312_length_8732_cov_4.433329"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLG78_06085"
     CDS             complement(<1..327)
                     /locus_tag="PLG78_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE22713.1"
                     /translation="MRFLVLIVLAACSVPTRSGPSYLGVLYQESREGVEVVQIYPGSP
                     ARTAGLAIGDTIVAVDGQRVGRSSSLRAAIMERRPGATIRLTVLRATGERADLDPRRR
                     AAVARGA"
     gene            1040..2551
                     /locus_tag="PLG78_06090"
     CDS             1040..2551
                     /locus_tag="PLG78_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNE22714.1"
                     /translation="MSLPESVLIANRGEIAVRIVRTCRKLGIRTIVSYSSADRNSLAV
                     RIADEAIEIGPPDSRSSYLNIEAIIAACKKTRAAAVHPGYGFLSENAEFASRLAAEGI
                     QFIGPSPDTIRSMGDKISARKLMQDKGVPVVPGYNGDDQSPGRLKEAAMSIGLPALIK
                     ASAGGGGRGMRVVTREEEFIPALEAAKREALGAFGDDRVFVEKYVSSPRHIEVQVLGD
                     SHGKVVALFERECSLQRRHQKIVEESPSPALTELLREQIMKAGVLAAEAVSYVNAGTV
                     EFIYSDTERTFYFLEMNTRLQVEHPVTEMVTGIDLVEQQLRVAGGLPFSFDPASLSQT
                     GHAIEVRLYAEDPEKNFLPATGKVHLFETPADVRVDSGIESGDEVTIHYDPMISKIIS
                     HGVNRAEAIDKMTKALDETVLFGPNNNLDFLRSIVRHPVFRSGKITTGFIPEHFPSFR
                     RTSSPDTVELARASASVLTYLGGKQISGSELNLAQTGGDLLPWIQIEGFRLWN"
     gene            2542..3342
                     /locus_tag="PLG78_06095"
     CDS             2542..3342
                     /locus_tag="PLG78_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE22715.1"
                     /translation="MELGSKVEVTREEVNGGTIVHVKFDQPETRNSLTLEMGLSFHRV
                     MHALAGEHPLPRACILSGKNGVFSSGGDFQLLKSFAEKTPKENTEFMQSFYRLFLTVR
                     HMPFPVLAAVNGHAIGAALAITLACDLRYFVPDAKYSLNFVRIGIHPGMGSSFLLKQI
                     AGMMRAQELILTGRYFSGTEAEQMGICHGTFPADTIVEKVTEIAREIALGAPRAVRLA
                     KRSLYEATDIENAIQLESHFQAETYQTQDFREALKAIEEKRTPTFKDE"
     gene            3348..4352
                     /gene="mltG"
                     /locus_tag="PLG78_06100"
     CDS             3348..4352
                     /gene="mltG"
                     /locus_tag="PLG78_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE22716.1"
                     /translation="MDKKKIIRIIIGAAAALLIGAGLFAIAGPGRPPGNGSKKVLFEI
                     ESGSGPGSVSERLAAEGLITNSAYLRFLLRTTAGGKIKMGVYEINDGMSAGEIASLIS
                     RGKVKMISITIPEGWHNRQIADEMVAKGLVKTTEEFLKLTEDPQVLAKYKIPAKTTEG
                     YLFPETYSVPLGYSATSFQDLMIRQFLKKAREAGAAENITPQELHEKVIMASIIEREA
                     LKREELPLMAQVFENRLEKRMKLESCATIQYLFERPHKKLYERDLVIQSPYNTYIHKG
                     LPPGPISNPGLPALRAAFHPEATDALFFVLKPDGSHHFSKTFKEHNEAKQKYLGGAPV
                     "
     gene            4349..4810
                     /locus_tag="PLG78_06105"
     CDS             4349..4810
                     /locus_tag="PLG78_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22717.1"
                     /translation="MKDVADAANSLKNMLSQLEKENKKLTGDLEVKRKERATRIKEAK
                     ATQKKMHSHLEKIDGHLAKGRAALDKQTGALAGIKKIVKQQEQTLKDLHLRLERLEHG
                     LRGGKMSIPDARRELMKMLEDLQGMKKQREKIMSSEGKVNATNFPSFRLDP"
     gene            4838..5278
                     /gene="rplM"
                     /locus_tag="PLG78_06110"
     CDS             4838..5278
                     /gene="rplM"
                     /locus_tag="PLG78_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNE22718.1"
                     /translation="MIILENQKTKVTKDPEIVKSWVLIDAKDQILGRLASKAAHRLRG
                     KHRVDFSENQDIGDFVVIINAKHIRTTGRKMEDKMYRHHSRHPGGLKEFNLKTILEKH
                     PTLAVEKAVKRMIPSGPLGRKIMGNLKIYAGAEHPHKPQKPTQI"
     gene            5294..5686
                     /gene="rpsI"
                     /locus_tag="PLG78_06115"
     CDS             5294..5686
                     /gene="rpsI"
                     /locus_tag="PLG78_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNE22719.1"
                     /translation="MAVKATLIVGRRKTAVARAKLTAGTGKFIVNGKDLKEFFPLARL
                     QKTAAQPLVLAGLEAKYDVSANVAGGGITGQAGAVRLSLARALQTVNPALHTDLKKAG
                     FLKRDPRMVERKKYGKRKARRGTQFSKR"
     gene            5759..5938
                     /locus_tag="PLG78_06120"
     CDS             5759..5938
                     /locus_tag="PLG78_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22720.1"
                     /translation="MQIESGSPGFEAISRSTRMLNELNQTIVDKTQEFQSKAVKMNVA
                     EQVTSSAENQIDLQA"
     gene            complement(5941..6393)
                     /locus_tag="PLG78_06125"
     CDS             complement(5941..6393)
                     /locus_tag="PLG78_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22721.1"
                     /translation="MQTFLISGTLIARGPFDFSHYSTLREGTRFKIQNGGEWIGKGQI
                     VSISRNQIRMVFEIHKSGVSVRGDVSLSHIRTEKQFEILALNYSGNVNGAPENSSEQV
                     KSDLFLCENGILAFRFLKGSRFFQLSVNRAGESSVTTDWGTGKLLPER"
     gene            complement(6440..8266)
                     /locus_tag="PLG78_06130"
     CDS             complement(6440..8266)
                     /locus_tag="PLG78_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22722.1"
                     /translation="MKFRPFPQILAFLILTALSADSSSLVIGTSYFAFSPNGDGIQDE
                     IEVRFLEIPSSLETPADYALRIENDRSLVRTIRADLRWIRPRRAVSNLFLPFAETVRP
                     LSFPESIPWDGRDDAGKILPDGNYRITVTAADASGRVSNSNSVTVELSTRRPQLTLSA
                     DVRAIVRGVTQDGKPADSTKGEFAISQSAQADPKTVFTGRIVNAAGEILETRTWPGTL
                     SPKIIWNGRTAKGDLVPWGCYTYWLEARSPAGVVVRESIPGLLVVTELPQIDLQPDES
                     FSPNGDSVKDKIQFLIQYFNQSLRAGVKSYRLQILTHDSPEIIYDTQPQRVLPGILVW
                     DGKNAAGAIVKDGIYDAKLTLETTQGTVSSAPRSFRVDTSPPGVSFSSSSSSFSPDGD
                     GEEEDISFKMDAADISGIEQWTIRVLVTPDTQNRFQKLIRTWQGAALPERIMWNGLSD
                     AGEPIESLETFTVVLEARDRAGNLSKPEKLVVRSKTLLRPVAPGQRALYARIPVQKYF
                     NADSTLTSQGEAAISDVLSHLSRFRKYNIHLYVNSGLPGGEEQNLEITERRALQLYKR
                     MQGSWKDRMDFQGLGESEILNPSPDDFSQYRNERIELQLYVP"
     gene            8317..>8732
                     /locus_tag="PLG78_06135"
     CDS             8317..>8732
                     /locus_tag="PLG78_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNE22723.1"
                     /translation="MAEKVLVAMSGGVDSAVAALLLIEQGYEVVGVNLRTWDYEAVAC
                     DTRKKSCCSPRDVQDARDTGIHLDIPFYVVRIEKAFKESVMDRFVLDYVSGRTPNPCV
                     ECNTFVKFGILFEKARSLGIDRIATGHYARTHAGKR"
BASE COUNT         2405 a         2219 c         2179 g         1929 t
ORIGIN      
        1 agcaccgcga gcgacggcag ctcgccggcg tgggtcgaga tccgctcgct cgcccgtggc
       61 gcgaagaact gtgagtcgaa tggtggcgcc aggcctgcgt tccatgatgg cggcgcgcaa
      121 agaagaagat ctacccactc tctggccgtc cacagcgaca atagtgtcac caatggcaag
      181 accagccgtg cgggccgggc ttcccggata gatttgcacc acttctacac cttcccggct
      241 ttcctgatag agaacgccca gataagacgg accgctcctg gtgggcacgg agcaagcagc
      301 caggacaatt agaaccagga atctcacaat gaattatcgg agatgggcct gcctgattgc
      361 aagacctaaa ccagtgcaat tgatttttta acgagaaaaa cggggaaaca acccacgctg
      421 aaaaagccgc cggggctcat ccaacgtggt caagagtcta gcggtcccgc ttggagcgtt
      481 tggaccaatt cttacgatat gcttgatctg aaacctgctt gggcttcaga ccatacttgc
      541 cagcaatctg accaggctcc tggcctgaac gatatgcttt ttcgatgctc gccttttgcg
      601 aggacgaaag gtctgtccaa tttttcttca tgcattgcct cactatctat ggggatgttc
      661 tttagaagcg ctaatttatc caaccggtca ataacaaaaa tgcgaaggag tgcgcgaaga
      721 tgaaaaaact tttcactttc gcacgcgtca cgaaaatgaa cgacatgttt ttggatatga
      781 acaacacttt cgcaaaaacg cgcgaaaatc gaaagcgaat gtgcggagaa aacttgtgcg
      841 aaaatgtgcg aagacttcat tcgcaacatg tgtgcgagaa gagacaagat gcgaacgcat
      901 tcgagtcatc atgcgaagct acagcaagcg gaaccttcat cgttggctcg agcatgaagt
      961 ttcaaaacca aaaaactggc atgcgaaatg cacaagcaat tgcaattgac attgccccgg
     1021 tcgcagcctt tctacgccca tgagtctacc tgaatcagtt ctcattgcga atcgcggcga
     1081 aatcgcggtg cgcatcgtgc gaacgtgccg caagctgggc attcgcacaa tagtttcata
     1141 ttcaagtgct gatcgtaact ctctggcagt tcgaattgca gatgaggcta ttgagattgg
     1201 gcctcccgat tcgcgtagct catacttgaa tatcgaagca attatcgcgg cttgcaagaa
     1261 gactagagct gccgcagttc atcctggata tggattcctg tccgagaatg ctgaatttgc
     1321 aagcagactg gctgcagaag gcattcaatt cattggtccg tcaccggata caattcgatc
     1381 catgggagac aaaatctccg ctcgcaaact catgcaagac aaaggggttc ccgttgtacc
     1441 aggatacaat ggagatgacc agagtcctgg acgactcaaa gaagcagcca tgtcgatcgg
     1501 gcttcccgct ctcatcaagg cttccgccgg gggaggagga agggggatgc gcgtggtcac
     1561 ccgagaagaa gagtttatcc cggcgctcga agcggcaaaa cgggaagcac tcggagcatt
     1621 cggcgacgat cgagtctttg ttgaaaaata cgtctccagt cccaggcaca tcgaagtaca
     1681 ggtgttaggc gattcgcatg gcaaagtggt cgctctcttt gaaagagaat gctctctgca
     1741 aagaaggcat cagaagatcg tagaagaaag cccgagccct gccctcaccg aacttctaag
     1801 agaacagatc atgaaagcgg gcgttctggc agcagaagca gtgtcatatg taaatgccgg
     1861 aaccgttgaa ttcatctatt cagacacaga acgcacattc tatttcctcg aaatgaatac
     1921 caggctccag gtggagcatc ccgtcactga aatggtcact ggaatcgatc tggtggaaca
     1981 acaactccgt gtggcaggcg ggctaccttt ctcctttgat cccgcttctt tgagccagac
     2041 ggggcatgcc atagaggtca gactgtatgc ggaggatccg gagaagaatt tccttccggc
     2101 tacaggcaaa gtgcaccttt ttgagactcc ggctgacgtt cgagtcgatt cgggcattga
     2161 gtcaggagac gaagtcacca tccactatga tcccatgatt tcaaagatca tatcccatgg
     2221 cgtcaatcgc gcagaagcga tcgacaaaat gacaaaggct ctagacgaaa cggttttgtt
     2281 tgggccaaac aacaacctgg acttcttgcg ttcgattgta aggcaccctg tatttcggtc
     2341 gggcaaaatc acgactggat tcatccccga gcacttccct tcttttagaa gaacttcttc
     2401 tccagacaca gttgagcttg cccgggcatc cgcctctgtt ttaacttacc ttggcggcaa
     2461 acaaatctcc ggatcagaac tcaatctggc tcagaccggc ggagatctgc taccgtggat
     2521 tcaaatcgag gggttccggc tttggaatta ggctcaaaag tagaagtaac aagagaagaa
     2581 gtgaatggcg gaactatcgt tcatgtaaag ttcgatcagc cagagaccag gaattcgctg
     2641 actctggaaa tgggtctttc ctttcaccgc gtcatgcatg cgcttgcagg cgaacatcct
     2701 cttccacggg cttgcattct atctggtaaa aacggagtct tctcaagcgg cggagatttc
     2761 cagctgctca agagctttgc tgagaaaact cccaaagaga acacagaatt catgcagtcc
     2821 ttctacagat tgtttctcac agtgcgtcac atgcctttcc cggttctcgc agcagtcaat
     2881 ggccatgcga ttggagcggc gctcgcgatc actctggcat gtgaccttcg ctattttgtt
     2941 ccggatgcga agtactcttt gaattttgtt cgcataggaa tccatcctgg aatgggatcc
     3001 tcttttcttc tcaaacaaat cgcaggaatg atgagagccc aggaactcat actcaccgga
     3061 agatattttt ccggaacgga agcagagcag atgggaatct gtcacggaac gtttccggca
     3121 gacaccattg tagagaaagt cacagagatc gcgcgcgaaa tcgcactcgg agcacccaga
     3181 gcagtgcgtc ttgccaaaag gtctctctac gaagcaacag acattgaaaa cgcaatccag
     3241 ctggaatcgc attttcaggc tgagacgtat caaactcagg atttcagaga agcactcaaa
     3301 gcaatcgaag aaaaaaggac acccactttc aaggacgaat aacgttcatg gacaagaaga
     3361 aaatcatccg aatcatcatt ggcgccgccg ccgcacttct cataggagcc ggactattcg
     3421 ccatcgcagg ccccggtcgc cccccgggca atggctcaaa gaaagtattg tttgaaatcg
     3481 aatccggaag cgggccaggc agtgtcagcg aacgcctcgc ggcagaaggg ctcatcacga
     3541 attcagcgta ccttcgattt ctgctgcgaa ccacagccgg cggcaagatc aaaatgggag
     3601 tctatgaaat caatgacggt atgtctgctg gtgaaatcgc ttctctgatt tctcgtggca
     3661 aagtaaaaat gatttccatc accatcccgg aaggatggca caacagacag atcgcggatg
     3721 aaatggtggc caagggtctg gtcaaaacga cggaagaatt tctcaagctg acagaagatc
     3781 cgcaggttct ggcaaaatac aaaattccgg caaagacaac agaagggtac ttgtttccgg
     3841 aaacatacag tgtgcctctc ggatattccg cgacatcgtt tcaagatttg atgatccgcc
     3901 agttcttgaa gaaagcacgc gaagcgggag ccgcagaaaa tatcacaccg caagaactcc
     3961 atgaaaaagt aatcatggct tccatcattg aaagagaagc gctcaagcgc gaagagctac
     4021 ctctcatggc ccaggtattt gagaatcgcc tggaaaagag aatgaaactc gaatcgtgtg
     4081 ccaccattca atacttgttt gagcgccctc acaagaaact atacgaaaga gatcttgtga
     4141 ttcaatcgcc ttacaacacg tacatccaca aaggacttcc tcccgggccc atctcaaatc
     4201 caggacttcc ggcacttcgc gcagcttttc acccggaagc aacggacgct ctcttcttcg
     4261 ttctcaaacc ggatggatcg catcactttt ccaaaacatt caaagagcac aatgaggcca
     4321 aacagaagta tctgggtggt gctcctgtat gaaagacgta gcagatgcag cgaactctct
     4381 aaagaacatg ctctctcagc ttgagaaaga gaacaaaaag ctgactggag atctggaagt
     4441 taagcgaaaa gaaagagcga ctcgcatcaa agaggcaaag gccacgcaga agaagatgca
     4501 cagtcatctg gaaaagattg atgggcacct ggcaaaaggg cgcgccgcgc tcgacaagca
     4561 gaccggagcg ctcgcaggca tcaaaaagat agtaaagcaa caagagcaga cgctcaaaga
     4621 cctccatctc aggctcgaac ggttagaaca cggactgcgc ggaggcaaaa tgtccatccc
     4681 ggatgcgcgc cgtgaactca tgaaaatgct cgaagatctg caaggaatga agaagcagag
     4741 agagaagatt atgtcctcgg aaggaaaggt aaacgcaacg aatttcccct catttcgcct
     4801 tgacccctga agcgtttcgg tacgattggc actggcaatg attattctcg agaaccagaa
     4861 gacgaaagtc accaaagacc cggaaatcgt taagtcctgg gtcctcattg acgcaaaaga
     4921 ccagatcctg gggcgtctcg cctccaaggc tgctcataga ctgcgcggaa aacaccgtgt
     4981 tgatttttca gagaatcaag acattggcga tttcgtagtc atcatcaacg cgaagcatat
     5041 tcgcactact ggaagaaaga tggaagataa gatgtatcgc catcactccc gccatccagg
     5101 tggactcaaa gaattcaatc tcaagacgat cctcgaaaag catccaactc tggctgtgga
     5161 aaaagcggta aaacgcatga ttccttccgg tcctcttgga cgcaaaatca tgggcaacct
     5221 gaagatttac gcgggcgcag agcatccaca caaaccacaa aaaccaactc agatctaata
     5281 tcaggaaagc atcatggctg tgaaagcaac tctcattgta ggcagaagaa agaccgcagt
     5341 agcgcgcgct aaattgacag cgggcacggg taagttcatt gtaaacggaa aggacctcaa
     5401 agagttcttc ccacttgctc gcctgcaaaa gacagcagct cagccactgg ttctggccgg
     5461 tcttgaagcc aagtatgacg tgtctgctaa cgtggcgggc ggcggaatca caggccaggc
     5521 aggagcagtt cgcctcagcc tcgctcgcgc tctgcaaaca gtcaatccag cactccacac
     5581 ggacctcaag aaggccggat tcctgaagcg cgacccgaga atggtggaaa gaaagaaata
     5641 cggcaagcgc aaagcacgcc gcggcacaca attctccaaa cgctgatttt tttcttcagt
     5701 aatttgcaaa atctgccgat catgtaaaag gcccaaatgt gcctgcacgg aggcagtcat
     5761 gcaaattgaa tctggatcac ccggtttcga agcaatcagt cgttccacgc gtatgctcaa
     5821 cgagctcaat caaacgattg tagataagac tcaagaattt caaagcaaag cggttaagat
     5881 gaatgttgca gagcaagtta catcatccgc cgaaaaccag atcgacctgc aagcataacc
     5941 tcagcgctcg ggcagtagct ttcccgttcc ccagtccgtg gtgacagagg actcgcctgc
     6001 ccgatttact gaaagctgga agaatcttga gcctttcaaa aagcgaaagg ccaggattcc
     6061 attctcacat aaaaacaagt ccgacttcac ctgctcactt gaattctctg gcgcaccatt
     6121 cacgttgccg gaatagttca aggccagaat ttcaaattgt ttttcagttc tgatatgcga
     6181 aagagaaaca tctccgcgga cgctcacgcc tgatttgtga atttcaaaca ccatacgtat
     6241 ctggtttcgt gaaatagaaa cgatctggcc tttaccaatc cattcgcctc cattttgaat
     6301 cttaaatcgc gtcccctctc gaagagttga atagtgcgaa aaatcaaagg ggccacgagc
     6361 aatcagagtg cctgaaataa gaaaagtctg cacgatcaaa caaaaagcaa ccgctcgcct
     6421 ccgccaggat actgcaaact caaggcacat acaactgaag ctcgatgcgt tcgtttctat
     6481 actgggaaaa atcgtctgga gaaggattga gaatttctga ttctccgaga ccctggaaat
     6541 ccattctgtc cttccaggaa ccttgcattc ttttgtacaa ctgcagtgcg cgacgttctg
     6601 tgatttccag gttttgctct tccccaccag gaagtccgga gttcacatat agatgaatgt
     6661 tgtatttgcg aaagcgtgac agatgcgaaa gtacatcgct gatggccgct tctccctgcg
     6721 aagtgagagt agagtccgca ttgaagtact tttgcacagg gattctggca tagagagctc
     6781 tctgccctgg tgccacagga cgaagcaacg tcttacttcg cacaactagc ttttccggct
     6841 tagaaaggtt accggctcga tcgcgggcct caagcaccac agtgaaagtc tccagggatt
     6901 caatcggctc acccgcgtcc gacaaaccat tccacatgat tcgctctggc aaggccgctc
     6961 cctgccatgt gcgaatcagc ttttgaaatc gattctgcgt gtccggtgtt actagaacgc
     7021 gaatcgtcca ttgttctata ccggaaatat ccgccgcgtc catcttaaaa gaaatgtctt
     7081 cttcttcccc atcgccatcc ggcgagaagc tggaagaact ggaagagaac gaaacgccgg
     7141 gtggtgacgt gtccacccga aacgatctgg gtgcgctcga tacagtgccc tgagtggtct
     7201 ccaacgtcag tttcgcgtcg tatatcccat ctttgacgat cgcccctgcc gcgttttttc
     7261 catcccacac cagaattcca ggaaggactc tttgaggttg tgtatcgtat atgatttccg
     7321 gcgagtcgtg agtcaaaatt tgaaggcgat aggacttcac accagccctg agcgattgat
     7381 tgaaatattg aatcaaaaac tggatcttgt ctttcaccga atcgccgtta ggcgaaaagg
     7441 attcatccgg ctgaaggtcg atttgaggaa gttctgtaac cacaaggagg ccggggatgc
     7501 tttcgcgtac gactacgcca gctggactgc gcgcttcgag ccagtacgtg tagcatcccc
     7561 acggaactag atcgcctttt gcggttcttc cgttccagat gatcttagga gaaagagtcc
     7621 cgggccatgt tcgcgtttcg aggatttccc cggccgcgtt tacgatcctt ccagtaaaaa
     7681 cagtctttgg atccgcctgc gcggattgcg agatcgcaaa ttctcctttc gtagaatcgg
     7741 ctggctttcc gtcctgagtg actccgcgca caatcgcgcg aacatccgcc gaaagagtaa
     7801 gctgcggcct gcgagtggaa agttccaccg taactgaatt ggaattgctc acacgcccgc
     7861 tcgcatcggc tgcagtgact gtgattctgt agttgccatc gggcaggatc ttcccggcat
     7921 catcccttcc gtcccacggt atgctttctg gaaaggagag aggacgaacc gtttctgcga
     7981 atggaagaaa caaattggac accgctcgcc tgggacgaat ccaccgtaaa tccgcccgaa
     8041 tggtgcgaac cagggaacga tcgttttcga tgcgcaaagc gtaatcggcc ggtgtttcga
     8101 gagaggatgg gatttccaga aaccgcactt cgatttcatc ctgaatcccg tccccgttag
     8161 gtgaaaatgc gaagtaggat gtgcctatga caaggctcga cgaatccgcc gacagagccg
     8221 tcaggatgag aaacgccaga atctgcggaa aaggtctaaa tttcaccgtt caaaaggaag
     8281 tgccgcacgc ccggtctttt tgtaaagtgg taaaacatgg cagagaaagt cctggttgca
     8341 atgtcgggtg gcgtggatag cgctgtggcc gcgcttctgt tgattgaaca gggctacgaa
     8401 gtagtgggtg tgaatcttcg cacgtgggac tacgaagcag ttgcctgtga tacaagaaag
     8461 aagtcatgct gtagccccag agacgtccag gacgccagag acactggtat tcacctggac
     8521 attccattct atgtagtgcg aattgagaag gctttcaaag agtccgtgat ggaccgtttt
     8581 gtgctggatt acgtttcagg aagaactccc aatccatgcg tagaatgcaa tacgttcgtc
     8641 aagtttggaa ttctatttga aaaggccaga tctcttggca ttgatcgaat tgcaacaggt
     8701 cactatgcgc ggacccatgc gggaaaaaga ca
//
LOCUS       DAOQJL010000098         8662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4360_length_8662_cov_4.182293, whole genome shotgun sequence.
ACCESSION   DAOQJL010000098 DAOQJL010000000
VERSION     DAOQJL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_8662_cov_4.182293"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLG78_06140"
     CDS             <1..630
                     /locus_tag="PLG78_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE22724.1"
                     /translation="LETAEGLKVGASVQILGVPSGVVDSMYYVPPEDSGETKVLAVLA
                     ITRQVPIYSNYRIITRFPTALAAKVVEINPGTRTNGAFQVQPRLLGSHELVNLKTSGI
                     MPEAGANVLRAANYDDPLYLIATSLTENRRSLRKIASNVAEITEKMNSGNGTIAALLN
                     RGDLVNNTNEILRGSLILIQEIREGVEDTRESKAAIDFISVVTAFVGLK"
     gene            699..1928
                     /locus_tag="PLG78_06145"
     CDS             699..1928
                     /locus_tag="PLG78_06145"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNE22725.1"
                     /translation="MAREFEIPLFYRSPILSVVKNARKNQDRHKRDFAPSVLDLGSMR
                     FKLARHFGFCYGVENAIEIAYRAIEENPGKRIFLLSEMIHNPQVNRDLTDRGVQFLMK
                     TDGTKLIPFEELRSDDIVIVPAFGTTLEMFDSLAKIGIDPQTYNTTCPFVEKVWNRSE
                     QMGSQGYTIIIHGFDNHEETRATFSHARRSAPSVMIRNMQEAIRLAAYVRGERDSASF
                     FEEFPGRYSIGFDPTKDFERIGVVNQTTMLATETQEIADYLKNAVIARAGAGNLSFHF
                     ADTRDTLCYATSENQESVYGLLESGGDLAVVVGGYNSSNTSHLVELCEKKLPTYYVKD
                     AEEILSRDRIRHLDLASGKIIETDGWLPVSSSRPVEILVTAGASCPDAVVDRVIDRMA
                     ALAGLRDRIEDALRVFA"
     gene            1959..2765
                     /locus_tag="PLG78_06150"
     CDS             1959..2765
                     /locus_tag="PLG78_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE22726.1"
                     /translation="MNWFQNVLVGILFFGALALAGFFTIISEAGPFAKKGNLLVLYFD
                     NADGVKVGSRVTVLGVPAGVVTDLALIGVDKAGLPLKEDSPDIVAQKVAVTAEIRRPV
                     KFYSNYKIAVKNETILSGKIIAIDPGSVRPMENGKIPELISVLAPSTTDLQKERKSAL
                     EHRLGQKGGFVDLQGESSGDPLAGLSEMISENRTDIRRTLNNVAEITTKINQGKGTLG
                     LLVNDDELHRNANTVLTDAQIVVRELREGLEDTREQAPVNSFLRAALTAF"
     gene            2781..4259
                     /locus_tag="PLG78_06155"
     CDS             2781..4259
                     /locus_tag="PLG78_06155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE22727.1"
                     /translation="MLYNSIEYFLFLSVLLVVYWKAPQKARNPILIVASLVFYAYWSI
                     AFLLHYVIILALNFPLFLAILKNRSKWAMRAALVLDFGNLIFFKYFYFMIRTIENFVP
                     AEYRGTLSQINPDNGEILPIIILPLAISFYTFQIVAFHVDAYRGEVTEAPGFWKYFLF
                     ISYFPQHIAGPIIRHHHLLKQLDEPRDPSKVDVHRALALIGMGVFKKVIIADTFAGMA
                     AQITRNPEKYDPAFVILGSVFFAIQVYSDFAGYSDIARGSSLLLGFELPVNFRGPFFQ
                     LSYQEHWQRWHITLSFWLRDYLYIALGGNRQGKFRSHLYLLITFILGGLWHGAGWGFV
                     IWGTLVGSLLVLERICRDLGWISVQETWGRRDQESSKSYSIRTVRNLSRMSLIFGLIV
                     AGGIFFNAGLDVSVAMRNFTAIFNPEADTKEFGLNSWHGLLFLAFLFLHWLEYQDFAP
                     VFRLREKYTHLERPVIAISAVLLMFLMSWKAAGNTSFVYFQF"
     gene            4267..5343
                     /locus_tag="PLG78_06160"
     CDS             4267..5343
                     /locus_tag="PLG78_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE22728.1"
                     /translation="MKNIYFVVGLSVLLLAIDKVALIPAFRQCCTTASSVSVFRLSLD
                     SDFTQDAAIERLRARKMRYFMNFGSSRSFGYYFNPTADQIKTTPYLSDNERAKLLQWD
                     AVNSAAPGASIVSEYVRMMQWLDHGARPDFISVELSRFSLNSSSLWLNDEIKNGIPAA
                     FAIRYAGQMPLPHSSTVVGSRVFALSRYRVGEPTAVSASIWEGLFSQITSNLSAEKVF
                     QQTTPGIPVGQESPVMLMSYNHVASEMRRTMFSHYHVDSNLRRYPFLMLARAKKENIP
                     IVFWNPAAHPVWLDKEKDCDVRDTYNLFLRELKREGGIYIDLSQDKMKCSEFSDPVHM
                     HIHCFPEMAWKKISALESAGALKL"
     gene            complement(5344..5658)
                     /locus_tag="PLG78_06165"
     CDS             complement(5344..5658)
                     /locus_tag="PLG78_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22729.1"
                     /translation="MIASLPAEKFRVIVKYALILSAIFLAASCSRKPAKEDCARYDRH
                     LAELAAHPAIEAALRSAGGRDAATAHCMELKKSQVECSTEATSLSDAAACESKKGSFF
                     DF"
     gene            5664..6287
                     /locus_tag="PLG78_06170"
     CDS             5664..6287
                     /locus_tag="PLG78_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22730.1"
                     /translation="MRRVLVCSAIPEEQEMLGWYVARDVQLVPLICGIGNVAAAVSLA
                     EYLLSQTPDEILFVGSAGAYHSQSQESAGFRAVASNVFFQRDVSSIKGESSKPPLMPL
                     VIETRRGPIAAGIAMRSGCEDGSVNCVDSVTLIPPGSEFEGLDFENMECFGLAWVAHK
                     KNIPFTAILAITNDVGPLGSDQWRANYRAYSRELQKRILNGLTSPDL"
     gene            6363..6689
                     /locus_tag="PLG78_06175"
     CDS             6363..6689
                     /locus_tag="PLG78_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22731.1"
                     /translation="MREGTSYRIRLTVAVYREKRLVYRNEMNLPTLYVRRSECRAHVK
                     KEIENRLRNSNFFLSPRVDFDLVRYADEASCNTYLRYRIVEADEAAVLAEKDFDHSGM
                     SLNQDA"
     gene            6686..8158
                     /locus_tag="PLG78_06180"
     CDS             6686..8158
                     /locus_tag="PLG78_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22732.1"
                     /translation="MTAALADELTRHKQAYLQLFRSVLAKRPMDAAAKEQLIQKLSAH
                     MDSVIRTTDDLIPRIPEFATMLGVGAAPLSTFIGTNFLQALQMVAKQQPQAAPVAPRP
                     SGKPSSFLVEIIQFSPSGLPAGSRFETAESGFLKLITPQGEVMSQSFLMSGDAGAPEI
                     SYDAAPSTPAASSPSPVQDQARGPAAPQAPRPPQGAEKSILKEILERFGNQIDIPEKL
                     VPKDLGDESAEVEIFEGEPDEEEATVSSAPASAASPGAPPAERPAAKRESSILREMVE
                     KFANDLDIHEKLVPRDLGDDSAEEDVFATQEEEAEEEPVATPVVDAPIPFPFTSYLDV
                     IKRLQEFQASGDQGAYRAWLANEASSEAKVLVAVRNFERKEKAGGAVNWSEEYYNLST
                     RLACKPAYVERVHKHIIKLSRVHALWNQLMQTARSAPPALMEDLKRLWPQIKLLFDEG
                     GTAAALSSRLQILLLAVPDPSRKASLKQTLDSYLQRLAAI"
     gene            8228..>8662
                     /locus_tag="PLG78_06185"
     CDS             8228..>8662
                     /locus_tag="PLG78_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22733.1"
                     /translation="MMKSRLSILLCSLFSLCSIQCSAAFLPGQQQLDGRVLAVWLIHP
                     PWNYSRFLFAVSALDTTMRSYRIDSATGALTQVTAFPTISGIGIDIHPTLSYVYLANN
                     TTDGVIAAQFDTAGALTSAGSINTGNSNTHRVKVHPAGNAIYF"
BASE COUNT         2031 a         2111 c         2243 g         2277 t
ORIGIN      
        1 ctggaaacag cagaaggcct caaggtgggc gcttcggtgc agattctggg agtgccttcg
       61 ggcgtagtcg attccatgta ctacgttcct ccagaggatt cgggtgaaac aaaagttctc
      121 gctgtgcttg cgatcactcg tcaggtaccc atttactcca actatagaat catcactcga
      181 tttccaactg ctctggcagc caaggtagtt gaaatcaatc caggtacgcg gaccaatggg
      241 gcttttcagg tgcaacccag gttacttggt tcgcatgagc tcgtgaattt gaaaacatcc
      301 ggtatcatgc cagaggccgg agccaatgta ttgcgcgcag ccaactatga tgatccgctc
      361 tatctgattg caaccagttt gactgaaaat cgcagatcgc ttcgcaagat cgcttctaac
      421 gtggcagaga ttactgaaaa aatgaattca ggaaacggaa ccattgcagc actcctcaac
      481 cggggggacc tcgtgaacaa cacgaatgaa atcttgcgcg ggtccttgat tttgattcag
      541 gaaattcgtg aaggtgtgga agatacgaga gagagcaaag ctgcgatcga ttttatttca
      601 gtggtgaccg cattcgttgg cctgaaatag aagtcgttcg ccattgattg cttgtttttt
      661 tcatttgcag tgccacgcgc gcggtgcttc cttagtttat ggcccgagag tttgaaattc
      721 cgttgttcta tcgatcgcct attctatccg ttgtgaagaa tgcgcgcaag aaccaggata
      781 ggcataagcg agattttgcg ccgtctgtgc tggatcttgg aagtatgaga ttcaagctcg
      841 ctcgacactt tggcttttgc tatggcgttg aaaacgctat cgaaatcgcc tatcgcgcca
      901 tagaggagaa tccggggaaa cgcatcttcc tgctgagcga aatgatccac aatcctcagg
      961 tgaatcgtga tctaacagat cgcggtgtcc agtttttgat gaagacggac ggaacaaagc
     1021 tcatcccgtt tgaggagctc agaagcgacg atatcgtaat tgttcctgca tttggaacca
     1081 ccctggaaat gttcgatagt ctggcaaaaa tcggaattga tccacaaact tacaatacta
     1141 cctgtccgtt tgtggagaaa gtctggaaca gatcggagca aatgggttcg caaggttaca
     1201 cgatcatcat tcacggtttt gataaccatg aagagactcg cgcaactttt tcgcatgcaa
     1261 gaagaagcgc accctctgtc atgattcgca acatgcagga ggcgattcgt ctggccgcgt
     1321 acgttcgagg ggaaagagac tctgcttctt tttttgaaga atttccaggt cgctattcga
     1381 ttggcttcga tccaacaaaa gattttgaaa gaattggagt cgtcaatcag accacaatgc
     1441 ttgcaactga aacccaggaa attgcagact atctcaagaa cgcggtgatc gcgcgagcag
     1501 gggccgggaa tctctctttt cattttgcgg atactcgcga tacgctttgc tatgccacgt
     1561 ccgagaacca ggaatctgtc tatgggctgc tagagtcagg tggcgacctg gccgtggttg
     1621 tgggcggata caattcttcc aatacatctc atctcgtaga gctgtgcgag aagaagctgc
     1681 ccacttacta cgtaaaggat gcggaggaga ttctctcccg ggaccggatt cgtcatctgg
     1741 atctggcctc aggcaagatc atagaaacag atgggtggct tcctgtaagt tcgtcgcggc
     1801 cagtggagat cctggtgacc gcgggtgcca gttgcccgga tgccgtggtg gatcgtgtga
     1861 tcgaccgtat ggccgctctg gctggactcc gggatcgcat agaggatgcg ctgcgtgttt
     1921 tcgcttgacc aggcaaactc tggaaagagg ctttcactgt gaactggttc cagaacgtac
     1981 ttgtcggtat cttattcttt ggcgcactcg ccctggccgg cttctttacg atcatctcgg
     2041 aagcgggccc ttttgcaaag aaaggcaatc tgctggtttt gtattttgac aatgcggatg
     2101 gagtgaaggt tggatctaga gttactgttc tgggtgtgcc tgccggggtt gtgacggatc
     2161 tggctctcat tggagtggat aaggccgggc tcccgcttaa agaagactct ccagacatcg
     2221 tcgcgcagaa agttgccgtt acggctgaga ttcgtcgtcc ggtaaaattc tatagcaatt
     2281 acaagatcgc tgttaaaaat gaaaccattc tatccggtaa gatcatcgcc atcgatccgg
     2341 ggtccgttcg tcccatggaa aatggtaaaa ttcctgaact aatcagtgtg ctggctccat
     2401 ctactactga tttgcagaag gagcgcaaga gcgctctcga gcatcgtctg ggacagaagg
     2461 gtggattcgt ggatctccag ggggaaagct caggagatcc tctggccggc ctttctgaaa
     2521 tgatttcaga aaacagaacg gacattcgtc gtactctaaa caacgttgca gaaatcacaa
     2581 ccaagatcaa tcagggcaag ggtactctgg ggcttctcgt aaatgacgat gaacttcatc
     2641 gcaatgcgaa tacagtcttg acggacgcgc aaattgttgt gcgcgagttg cgcgaaggtc
     2701 ttgaggatac tcgtgagcag gcgccagtta acagcttcct gcgcgcggct ctgaccgcat
     2761 tttaattctg aaggcaactc gtgctctaca atagcattga atatttctta tttctttccg
     2821 tacttcttgt agtttattgg aaggcacctc agaaagctcg caatccaatt ctgattgttg
     2881 cgtcacttgt cttctatgca tactggtcca tcgcctttct actgcactat gtcattatcc
     2941 tggcgttgaa ctttcctctc tttctggcca tcctcaagaa tagatccaaa tgggccatga
     3001 gagcagcgct agttctcgat tttggaaact taatattctt taagtatttc tatttcatga
     3061 ttcgaactat tgagaatttc gttccggcag aatatcgcgg gacgctttct caaatcaacc
     3121 cggacaacgg tgagattctt cctatcatca ttctgcctct cgcaattagc ttctacactt
     3181 ttcagattgt tgcctttcac gtggatgcgt accgcgggga agtcacagag gcgcctgggt
     3241 tctggaaata ttttctcttc attagctatt ttcctcagca tattgcgggc ccgatcattc
     3301 gacaccatca ccttctcaag cagttagatg aaccacgcga tccatcaaaa gtagatgtgc
     3361 atcgcgcact ggcactcatt ggtatgggtg tgttcaagaa agtgatcatc gcagatacgt
     3421 ttgccggcat ggcggcgcaa atcactcgga acccggaaaa gtatgacccg gcattcgtga
     3481 tccttggaag tgtgtttttt gcgattcaag tttattctga tttcgcgggg tactccgata
     3541 tcgctcgtgg atcttctctc ttgctcgggt ttgaacttcc tgtgaatttt cgcgggcctt
     3601 tttttcagct cagttaccag gagcactggc agaggtggca catcacactg tctttctggt
     3661 tgagagacta tctctacatt gctctgggtg ggaatcgcca gggcaaattt agatctcatc
     3721 tctaccttct catcacgttc atactcggtg gtctgtggca cggcgcgggc tggggctttg
     3781 tgatctgggg gactctggtg ggttctcttc tggttctcga acgcatttgt cgtgatttag
     3841 gatggatctc ggttcaggag acgtggggca ggcgtgatca ggagtcgagt aagtcttaca
     3901 gcattcgcac ggtgcgaaac ctgtcgcgca tgtctttgat cttcggtttg attgtggccg
     3961 gaggtatttt cttcaacgcc ggcctcgatg tatcggtggc tatgcgcaac ttcactgcga
     4021 tttttaaccc ggaagcggat acaaaagagt ttggtctgaa ctcctggcac ggactcttgt
     4081 ttcttgcatt cttgtttctc cactggctcg agtatcagga ctttgctcct gtctttcgcc
     4141 tgcgcgaaaa gtatacgcat ctggagagac cggtcattgc aatttccgct gtgctcttga
     4201 tgttcctgat gagctggaag gcagcgggaa atacttcctt cgtgtatttt caattttgag
     4261 gcagagatga agaatatcta ttttgtagtg ggtctttctg ttcttcttct ggccatagac
     4321 aaggtggctt tgatcccagc atttcgtcag tgctgcacca ctgcatcgag tgtaagcgta
     4381 tttcgtcttt ctctggactc agacttcacg caggacgcgg ccattgaacg ccttcgcgct
     4441 cgtaagatgc gctattttat gaactttgga agttccaggt cgtttggcta ttacttcaac
     4501 cctaccgcag accagatcaa aaccactcct tacctttctg acaatgagag ggcgaaactg
     4561 ttgcaatggg acgcagtgaa ttctgcggca cccggggcgt ccatagtaag cgaatacgtg
     4621 cgcatgatgc agtggctcga tcatggagcc cgcccggatt tcatctctgt tgaattatcg
     4681 cgtttctctc tgaactcctc cagtctgtgg ctgaatgatg aaatcaagaa tggaatcccg
     4741 gccgcttttg ccatccgtta tgcaggacag atgccgctgc ctcattcatc tactgtagtt
     4801 ggaagtcgtg tgtttgcatt atccaggtat agagtgggcg aacctactgc agtatctgcg
     4861 tctatctggg aaggtctctt cagtcagatc acgtccaacc tttccgctga gaaagtgttc
     4921 cagcaaacta cgcccggtat cccggttggc caggaatcgc ctgtaatgct catgagttac
     4981 aaccatgtag cttccgaaat gaggcgtacc atgttttcgc actatcatgt ggattcgaat
     5041 ctcaggagat atcctttctt gatgctcgct cgcgcgaaaa aggaaaatat cccgatcgta
     5101 ttctggaatc ctgccgcgca tcctgtgtgg ctcgacaaag agaaggactg cgatgtccgc
     5161 gatacgtaca atttgtttct gcgcgaacta aagagagagg gtggtattta catagatctg
     5221 agtcaggaca agatgaaatg ctcagagttc agcgatccgg tgcacatgca cattcattgc
     5281 tttccggaaa tggcgtggaa gaagatcagc gcccttgagt ctgcgggcgc gctcaagctc
     5341 tagttaaaag tcgaagaaag agcctttctt gctttcgcag gctgcagcgt ccgaaaggga
     5401 cgtggcttcc gtagaacatt ctacctgtga ttttttgagt tccatgcaat gtgcggtggc
     5461 agcatctctt ccgcctgcag aacgaagagc cgcttcgatg gcagggtgag ctgcgagttc
     5521 cgcaagatgg cgatcatacc tggcgcagtc ttctttggca ggtttgcggc tgcaactggc
     5581 cgcaagaaag atggcagaca aaatcagagc gtacttcaca atcacacgga atttctcagc
     5641 gggcagggag gcaatcacaa aagatgcggc gtgtacttgt atgttcggcc attccagaag
     5701 aacaggaaat gcttggatgg tatgtcgcgc gagacgtgca gctcgtgcct ttgatttgcg
     5761 gcatcggcaa tgtggctgcc gcagtaagcc tggccgagta tcttctctcc cagacgccag
     5821 atgaaatcct gtttgtggga tctgccggtg cgtatcattc gcagtcacag gagtctgcag
     5881 gatttcgtgc ggttgcatcc aatgtatttt ttcagcgaga cgtttcttct ataaagggag
     5941 aatccagcaa accacctctg atgccgctgg tcatcgaaac tcgcagaggt ccaatcgccg
     6001 caggcattgc aatgcgatcc ggttgcgaag atggcagcgt aaattgcgtg gattctgtta
     6061 ctctcattcc acccggatct gaatttgaag ggcttgactt tgagaatatg gaatgcttcg
     6121 ggctggcctg ggtggcgcac aaaaagaaca ttccattcac tgcaattctc gcgatcacaa
     6181 acgatgtggg tccactcggg tcggatcagt ggcgtgcaaa ctatagagcg tactctcgtg
     6241 aattacagaa gagaattttg aacggcctta cgtctcctga tttgtaacgt ttccaaaacc
     6301 accccgaaaa atcatcgctg cctctttatt tcgggttctg cgcgggacga tcttgtgaat
     6361 gcatgagaga aggaacatct taccgaatcc gcctgactgt ggcagtttac cgtgaaaaga
     6421 ggctggttta tcgcaatgag atgaacctgc ccactctgta tgtaaggcgt tcggaatgca
     6481 gagctcatgt gaaaaaagag atcgaaaatc gcctccgcaa ttctaacttc tttctgtctc
     6541 cccgggtaga ctttgatctc gtgcgctatg cagacgaagc ttcctgcaat acgtatctgc
     6601 gctacagaat cgtggaagca gatgaagcgg cagtccttgc agaaaaagac tttgaccatt
     6661 ccggcatgtc cttgaatcag gatgcatgac tgcggcgctc gcagatgagc taacaagaca
     6721 caagcaggcg taccttcagc tgtttcgatc cgttctggcc aagcgtccca tggatgcggc
     6781 ggccaaagaa cagctcatcc aaaaactatc cgctcatatg gattcagtca ttcgcacgac
     6841 ggatgatttg atccctcgca ttccggagtt tgcaaccatg ctcggcgttg gagccgcgcc
     6901 cctctctact tttataggta caaactttct gcaggcgctg cagatggtgg caaagcagca
     6961 acctcaggca gcacccgttg caccgcgtcc ttccggtaag ccctctagct tcctcgtgga
     7021 aatcattcag ttttcgccgt caggccttcc tgccggatcg cgattcgaga ctgcagagtc
     7081 tggattcttg aaattgatca cgcctcaggg cgaagtgatg tcgcaaagtt ttctcatgag
     7141 cggagatgcg ggagctcccg aaatttctta tgacgcggca ccttcaactc cagctgcatc
     7201 ttcgccttcg cctgttcagg atcaagcacg tggtccagca gccccacagg caccgcgtcc
     7261 tcctcagggc gcagagaaat caatcctgaa agaaatcctg gagcgattcg gcaaccagat
     7321 cgacattccg gaaaaacttg taccaaaaga tctgggagat gaatctgctg aagttgaaat
     7381 ctttgaaggg gagccagacg aagaagaagc cactgtttca tccgcccctg catctgccgc
     7441 atcacctggc gccccccccg cagagcgtcc cgccgcgaaa agagagagtt ctattctgcg
     7501 cgagatggtg gagaagttcg ccaatgatct ggatattcat gaaaagctag ttcccaggga
     7561 tctgggtgac gactctgcgg aagaagatgt attcgctacg caggaagagg aagcagaaga
     7621 agaacctgtg gctactcctg tggtggatgc gccgattcca tttcctttta cttcttatct
     7681 ggatgtgatc aagcggcttc aggaattcca ggccagcggc gaccagggcg cttatcgggc
     7741 ctggcttgcg aatgaagctt catctgaggc aaaggtgctg gtcgctgtga ggaacttcga
     7801 aagaaaagag aaagcgggcg gagctgtgaa ctggtcggaa gaatactaca acctctccac
     7861 tcgtcttgca tgcaagcctg cgtatgtgga gcgcgtgcat aaacacatca taaagctatc
     7921 gcgagtgcat gcgctatgga atcaactgat gcagacagcc cgaagcgcgc ccccggctct
     7981 catggaagat ctaaaaagac tctggcctca aatcaagttg ctcttcgatg aaggagggac
     8041 ggcggccgca ctgtcctcga gacttcagat tctgctcctg gctgttccgg atccttccag
     8101 aaaagcttct ttgaagcaga ctctggattc ttatctacag aggcttgcgg caatttgata
     8161 tagaagtaat cgcttgcgct tgctcgatcc ggggtgattt tattacttaa gaagcaagga
     8221 acataagatg atgaagtcgc gtttgagcat actattgtgt tcgctttttt ctctctgttc
     8281 catccaatgc tctgctgcat ttctaccagg gcagcaacag ctcgacgggc gcgtcctggc
     8341 tgtgtggctc attcatccgc cctggaatta cagtaggttt ttgttcgcag tgtccgcgct
     8401 cgatacaacc atgcgttcgt atcgcattga ttccgcaaca ggcgcactga ctcaggttac
     8461 tgcctttcct acgatctcgg ggatcggaat cgacattcat cccacgctgt cctatgttta
     8521 tcttgcaaac aatacaacgg acggagtaat cgcggcgcaa tttgatactg cgggcgcgtt
     8581 gacttctgcg ggttccatca atacggggaa ttcgaacact caccgcgtta aagtccatcc
     8641 ggcaggaaat gcaatttact tc
//
LOCUS       DAOQJL010000099         8636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4380_length_8636_cov_4.173523, whole genome shotgun sequence.
ACCESSION   DAOQJL010000099 DAOQJL010000000
VERSION     DAOQJL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_8636_cov_4.173523"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLG78_06190"
     CDS             <1..1190
                     /locus_tag="PLG78_06190"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNE22734.1"
                     /translation="YGFANEGLWPLCHLAHARPMFRATDFRHYSRINARFAAAAAQEG
                     KVRDPIVWIQDYHFALAPQLVRKKLPAATILTFWHIPWPNAERIGICPWHKELIAGLL
                     GSDILGFHTQLHCNNFLSAIDRFLETRIDRERQSVFYQGHETIVRPYPISVEWPNSWA
                     VAAPDVSECRHALITECNLKQDAVIGLGVDRLDYTKGIAERFLAVERALERFPELRGR
                     FSFVQLAAPSRTEIERYRELSSQIEQIVERINARFGRGTYRPIVYHRAHHEPPKVFQF
                     YRACDFCYVSSLHDGMNLVAKEFVAARDDERGVLILSNFAGASRELSSALIVNPYDLE
                     EASSAFATAVTMSSEEQQERMRAMRQTVAKFNVYRWAGRMMMDTAGLRQHTRLLSRYG
                     DRD"
     gene            1187..1921
                     /gene="otsB"
                     /locus_tag="PLG78_06195"
     CDS             1187..1921
                     /gene="otsB"
                     /locus_tag="PLG78_06195"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNE22735.1"
                     /translation="MKDILSRKHVNELSLLIARKPLIAFDFDGTLAPIVDDRDSACMR
                     ASTRRLLRRLSRCLPCVVISGRMRSDVCARLEGIPLVAIVGNHGSEWEGDDHDHALLE
                     RVRNWKELLKGELPRDGVSIEDKSVSLSVHTRHNVSSPDIRRILNRLTDAKVIGGKRV
                     YNIVPAEAGGKGEALLRMMQECKCDCALYIGDDDTDEDVFALFDSEIMTIRVGQSRNS
                     SAAYYIASQAQIDTLLEKMLSWPPCS"
     gene            2030..3745
                     /locus_tag="PLG78_06200"
     CDS             2030..3745
                     /locus_tag="PLG78_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE22736.1"
                     /translation="MRQDPENNDGVIARSFARHRLLFLAVLTGLTAGLAAVSLKILVH
                     LVSGISTNRLPGSVFSFLPAAGLLLTIVFTRFVLRAQPGTTADILVTIVQRSSVVERF
                     KMISHLVGSALTVGLLGSAGLESPIAITGAAIGSNLGRNVNYKERTLLLSCGAAAGIA
                     AVFNAPVAGMLFAIEVLLLDVSLSSIIPLVAAAVTGSLCSIVLQQNEILFEFPRIQHF
                     DYRNIPFYFILALFCSMISLLYSGIMRLAQRFLSPFPWLLKWGVAGLILWAVGSIAPV
                     VLGEGSRAIAGLTQGLPREFFHSIPLPVPFAVALCIFVVLKIIATAATLKSGGIGGNF
                     APSLFVGACAGFALANAFNAAHPGLLPEGNFTIVGMAGTLSGVMYAPLTAVFLIAEAT
                     GGYTLMVPLLLVSALTHVIVIQLRSHSMELSELEEQGVNPTRRRDAQILGQIRLGDLL
                     EKDFVTVFEDAKLRDLIGAIAISRRNLFPVVTSSGDLVGIVTLNDIRDQIFKSELYDS
                     QFVRDLMVPIRQSIDINDSMQMAMDKFDHSGAWNLPVVESKRYAGFVSRSRLFAAYRE
                     GARSV"
     gene            3873..4541
                     /locus_tag="PLG78_06205"
     CDS             3873..4541
                     /locus_tag="PLG78_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE22737.1"
                     /translation="MHFVGINLVIALSLISCSMELSTGSQELLQKSSVVLLARLSSLT
                     STPVIICKNQGYCIVFRTTSTFAGTAVGGVSGADTKCNADANKPANTGTYKALITDGA
                     ARVACTSANCATSGIAEHSDWVMYPATEYRQVNQTTVIFTTNANGIFILPGALAGWDG
                     TASSFNSGFNSNWTASGGNHCTAWTIGGGSGMQGIGNSTSATMLQNASIGCGSVVSLL
                     SVQQ"
     gene            complement(4581..4919)
                     /locus_tag="PLG78_06210"
     CDS             complement(4581..4919)
                     /locus_tag="PLG78_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22738.1"
                     /translation="MKSGSGAFNIFGLIFCVFMGYTGVSLGFGAAYPPLNLVAKPFVC
                     SNGEMQYQQKSINPLPGRTYTMARWQCTDSSGNSAPVSNAHIAVYSGLIYGIGMFVLL
                     KLLMIFRGRS"
     gene            complement(5100..6047)
                     /locus_tag="PLG78_06215"
     CDS             complement(5100..6047)
                     /locus_tag="PLG78_06215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNE22739.1"
                     /translation="MMISAIIGLLDGQTGCTYFINAEHPSPVLFREGQVTFAANGQSC
                     FKLGSPMQATSARVFTMRLLPGDVLICGSDGRDDLRLADSAGINTDETLFLRLVADAN
                     GDLNALEDTLRRTGEIMDDLSLIRIAYKGHRSEESEGSFDREIENFLKQTRRQRRKHV
                     DDLCRELKFEIALKLLEGAALKYPWELEMLGRLAYVLRKTGHLQRAADLGESIRLREP
                     DNADFLWELARTQGALGNRDRVRTLLEGIPAEHALYTRAREGEACASSILGLIAFGDA
                     SLSAAAQNGGITTVKIVDYSYSNVLGALWTGSCTIAHGD"
     gene            complement(6044..6811)
                     /locus_tag="PLG78_06220"
     CDS             complement(6044..6811)
                     /locus_tag="PLG78_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE22740.1"
                     /translation="MDTPSTPLSIGYIDVSKLRLMTVAAAAQAVVLVAALSFVFATYL
                     NQFVLYLTRLLGRVASGDLTAKMTMASTDEIGALSTDFNRVTRSLRNARGQLESYATS
                     LEEKVQIRTEQLNATLSEVRTVKEKQDGDYYLTSLLLKPFLRAGLQTHSVEAAALISQ
                     FKKFEFRHFKEQIGGDSVTLDHVRLQERDYVFFMNSDAMGKSMQGVGGALVLGAVMHA
                     YLQRTKVSRQAHDVFPESWLNRLRRNCREFSRVSKGP"
     gene            complement(6771..7382)
                     /locus_tag="PLG78_06225"
     CDS             complement(6771..7382)
                     /locus_tag="PLG78_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22741.1"
                     /translation="MTRLTANLEAVTYLLGVPLGILFVIFQAGFSGQKLIIFLVSATV
                     GVLASMLYPVLRWRVLAPDINACIEGQVHGRQRRNLLRRLLNYPFWETIGVNIQWAGG
                     IAVVVLTNHLLEPLSMREFLPYVFVFIFLAPINAVSHYLAMQLSLAELIRSNRIFHGV
                     FLRRQDLVIVSLFRKTLGTMFSGVWLLLVFFGYTLYAALHWIH"
     gene            complement(7636..>8636)
                     /locus_tag="PLG78_06230"
     CDS             complement(7636..>8636)
                     /locus_tag="PLG78_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22742.1"
                     /translation="FLLGRGADRLTSFADLRGLRIGGGPVGSGTAYLSESIFGMPSMK
                     SLGVKLSFHPSDEQLRLIKEGKLDLGVFVISEKSEFIKKALLVEGLQIAGLRGLESVT
                     MNLAHLQARTMHQGLYDPVRNIPPVDKPVLSVNTLLVTNQEARRSEIVGMLQVMSELY
                     PNFINYNRTVVNETALNLPAGALDFYNNQGAEIFDRYLPRLMDLIPISNLVQLVMGVS
                     ILFNLMGLANRFILWRIDANRIAIEEEIEQVFGSNVLPEEIEIMNPLPEHAKGEGLHR
                     LNALIGHLTGLESKCRKQSQSILVPMGAEMAYRYQEKLISTNLIAIKKYKLKLERL"
BASE COUNT         1994 a         2192 c         2261 g         2189 t
ORIGIN      
        1 actatggttt tgcaaatgaa ggtctctggc ccctctgtca cctggctcat gcacggccaa
       61 tgtttcgcgc aaccgacttt cgccattatt cgcgcatcaa cgcccgattt gccgcggccg
      121 ccgcgcaaga aggaaaggtg cgcgatccaa tcgtatggat ccaggattat catttcgccc
      181 tcgcgccgca acttgtccgc aagaaacttc cggcagcaac gattttgact ttctggcaca
      241 taccgtggcc aaatgcggaa cggatcggca tttgtccctg gcataaggaa ctgattgctg
      301 gacttctggg cagtgacatt ctgggtttcc acacgcagct tcattgcaac aacttccttt
      361 ctgccattga tcgattcctg gagactcgaa ttgatcgaga aagacaatcc gtcttctatc
      421 agggtcacga aacgattgtt cgcccttatc caatttcggt ggaatggccg aattcctggg
      481 cggtggcggc ccctgatgtt tcggagtgca ggcatgctct gataacggaa tgcaatctga
      541 agcaagatgc tgtcattggt ctgggcgtgg acaggctgga ttacacgaag ggaattgcgg
      601 aaagattcct cgcagtggaa agagctctgg aaagatttcc tgagcttcgc ggccgtttca
      661 gtttcgtaca gcttgctgca cccagtcgga cggagattga gaggtatcgt gagctttcca
      721 gtcaaattga gcagatcgtt gaaagaatca atgctcgatt tggccgcggc acctaccggc
      781 ccatcgtgta tcaccgtgcg catcacgaac ctccgaaagt atttcagttt taccgcgcct
      841 gtgatttttg ttatgtgagc agtctgcacg atggaatgaa cctggttgca aaggaatttg
      901 tggcagcgcg cgatgacgag cgcggcgttc tcattctcag caacttcgcg ggagcttcac
      961 gcgaactcag ttccgcattg atcgtaaatc catacgatct cgaagaagcg agctccgctt
     1021 ttgcaacagc ggttaccatg tcttccgaag agcagcagga aaggatgcgt gcgatgcggc
     1081 agactgttgc caaattcaat gtgtacagat gggcgggcag aatgatgatg gatactgccg
     1141 gtctgcgcca gcatacacgt ttgttgtcgc ggtatggaga tcgggattga aggacatcct
     1201 ctcccgaaag cacgtcaatg agctgtcact cttgattgcc aggaaacctt tgatcgcttt
     1261 tgattttgat ggtacgctcg ctcccatcgt ggacgatcgt gattcagcgt gcatgcgggc
     1321 ctccacgcgc cgactactga gaaggctcag cagatgtctt ccctgcgtag taatttctgg
     1381 ccgcatgcgc tcagacgtct gcgcacggct ggagggcatt cccctggtcg ccatagtggg
     1441 aaatcacggg tctgaatggg aaggggacga tcatgaccat gctctgcttg agagggtgcg
     1501 gaattggaag gaattgctca aaggcgagct gccgcgcgac ggtgtttcta tcgaggataa
     1561 gagtgtttca ctctccgtcc acacgcgcca caatgtctca tctccagaca ttcgtagaat
     1621 tttgaatcga ctaacggatg cgaaggtgat cggaggtaag cgtgtgtaca atatcgttcc
     1681 ggcggaagct ggcggcaaag gcgaggccct gttgagaatg atgcaagaat gcaagtgcga
     1741 ttgcgccctc tacattggag acgacgacac ggatgaagac gtattcgcac ttttcgattc
     1801 cgaaatcatg accattcgcg taggccagag cagaaattcg agtgctgcct attatattgc
     1861 cagtcaggcg caaatcgaca cgctgctcga aaagatgctt tcctggcccc cctgctcata
     1921 gtgcaatggg ttgcgattcg gagaagcttt ccaatgccac ggtcactgac tccggaggaa
     1981 aattgcaaga atctatcgac agcgatggag tattcgttat tgcggagact tgcgtcaaga
     2041 tccagaaaac aacgatggcg tgattgcccg atctttcgcg cgtcatcgtc ttctatttct
     2101 cgctgttttg accgggctca ctgcgggtct ggcggcagtt agcctcaaga tactcgtaca
     2161 tttggttagt gggatcagta cgaatcgtct gcccggcagc gtcttctctt ttcttcctgc
     2221 agccggtctc cttctgacta tagtctttac gcgctttgtt ttacgagcgc aaccgggaac
     2281 cactgcggat atcctcgtta caatcgtgca gcgctcgagc gtcgtagagc gttttaagat
     2341 gatctcgcat ctcgtgggga gcgccctcac ggtcgggctt cttggatcgg cgggactcga
     2401 gtctccaatc gcgattactg gtgccgcaat tggttcgaat cttgggcgta acgtaaacta
     2461 caaagagaga acactcctcc tttcttgcgg agcggccgcg ggcatcgcgg ccgtttttaa
     2521 tgcccccgtt gcgggaatgc tctttgcgat tgaagttctg ttgctggatg tttccctctc
     2581 gtcaatcatt cctctggtgg cggctgccgt gactggctct ttgtgttcga ttgtgctgca
     2641 gcagaatgaa atcctctttg agtttccccg gattcaacac tttgactatc gtaatattcc
     2701 cttctatttc atacttgctt tgttttgctc aatgatctca ctcctttatt ccggtatcat
     2761 gcgcctcgcg caaaggtttc tttcgccatt tccgtggttg cttaaatggg gggtggctgg
     2821 cctgattctt tgggcggtcg gaagcattgc gccggtcgta ctaggagaag gttctcgcgc
     2881 gatcgccggt ctgactcagg gattaccacg ggaattcttt cactctattc cgcttccggt
     2941 tcctttcgcc gtagctctct gcattttcgt tgttctgaag atcatcgcga cggccgcgac
     3001 gttgaagagc ggaggcatcg gcggcaattt cgctccgtcg ctttttgttg gtgcctgcgc
     3061 tggttttgca cttgcgaacg cgttcaacgc ggcccatcca ggactcctgc cagaaggaaa
     3121 ttttacgatc gtcggcatgg ccggaacgtt aagcggcgtt atgtatgccc cgctgaccgc
     3181 ggttttccta atcgccgaag caacgggtgg gtatactctc atggttccgt tgctcctcgt
     3241 ttcagcgtta acgcacgtga ttgttattca attgaggtcg cattcgatgg aattgagcga
     3301 acttgaggaa cagggagtga atccgacccg gcgtcgcgat gcacaaattc taggccaaat
     3361 tagacttggg gacctgttgg agaaagactt cgttaccgtc ttcgaagatg caaagctcag
     3421 agatcttatc ggtgcaattg cgatctcgcg ccgcaatcta tttccggtcg taacttcgag
     3481 tggggatctt gtcggcattg tgactttgaa cgatattcga gaccagattt tcaaaagtga
     3541 actgtacgat tcacagtttg tcagagactt gatggttccc atccgccaga gcatcgatat
     3601 caacgatagc atgcagatgg cgatggacaa gtttgatcac agtggtgcct ggaatcttcc
     3661 cgtagtcgaa agcaagcgct atgcgggctt tgtgtcccga tcgcggttgt tcgcagcata
     3721 tagagagggc gcgcgaagtg tatagaaatg atgcccgact tttgtctgtt ctgccgcggg
     3781 catttctgcg cgcgacaaaa aagcatcgcg cgcgcaaaaa cagattcgac gattctttcg
     3841 ccctgtgcga aagttgaaga atgcggcggc tgatgcattt cgtcggtatc aacctggtga
     3901 tcgccctttc tctcatttca tgctccatgg agctttcgac aggaagtcag gagcttttgc
     3961 aaaaaagttc tgtcgtgttg cttgccagat tgagcagtct tacctctaca ccagtcatca
     4021 tctgtaaaaa tcaaggttat tgcattgtat ttcgcacaac gtccactttc gcaggcactg
     4081 cagtaggtgg agtcagcggc gctgatacaa agtgcaacgc ggatgcgaat aagcctgcaa
     4141 acaccggtac ctacaaggca ctcatcacgg atggcgccgc acgcgtcgcc tgcacttccg
     4201 caaattgcgc gacgtcagga atagcagaac attcagactg ggtgatgtat ccagctacgg
     4261 aatatcgtca ggtgaatcag accacggtca tttttacgac gaacgcaaat ggaatattta
     4321 ttcttccggg tgcccttgcg ggctgggatg gcacagcgag ctcattcaat agcggattca
     4381 actctaactg gaccgcgagc ggtggaaacc actgtacagc gtggactata ggcgggggat
     4441 ccggaatgca gggtataggg aactctacaa gcgcgaccat gttgcagaat gcatccattg
     4501 gatgcggttc ggttgtgagt ctactcagtg tgcagcaatg atgcgccacg cttgcgatct
     4561 ttgagcggac cgggggatgt tcagcttctt ccgcgaaaga tcataagcaa tttgagaaga
     4621 acaaacatgc ctattccgta gatgagtcca gagtagacgg cgatgtgggc attggaaaca
     4681 ggagccgaat tgccactcga atccgtgcat tgccatcttg ccatagtgta agttcttccg
     4741 ggcagagggt tgatggactt ctgctggtat tgcatttctc cattcgagca tacaaatggc
     4801 tttgctacta gattgagcgg ggggtaggca gccccaaaac caagggagac acccgtatat
     4861 cccataaaca cgcagaaaat aagcccaaag atgttgaagg cgcctgaacc tgatttcata
     4921 cgacccctct gtgcaaattg atttgcagat cagagattga tggagcgtgc agcctcagtc
     4981 cagttctttt tgtgaaaatt agttttgtat agaagaaagg ccgggctttc gcactgaccc
     5041 tgggggcagg cccggccggg aaaatagcag gagcaggttt gccgctcctg ctcaaagaat
     5101 caatcgccgt gtgcgattgt gcaagaaccc gtccagagag ccccgaggac gtttgagtag
     5161 ctgtaatcaa cgattttgac ggtagtgatt ccaccgtttt gcgctgcagc agaaagcgat
     5221 gcgtcaccaa atgcaatcag tccaagaata gatgatgcgc aagcttcgcc ttcgcgggcc
     5281 ctcgtgtaaa gcgcgtgttc cgccgggatt ccttccagaa gagtgcgcac gcgatctcga
     5341 tttcccaggg cgccctgagt gcgcgcgagc tcccagagaa aatctgcatt gtcaggctct
     5401 cgtaatcgaa tcgattctcc caggtccgcc gctctctgca ggtgacccgt tttcctcaga
     5461 acatacgcga gtctgccaag catttctaat tcccacggat acttgagtgc tgccccttcc
     5521 agtagcttca aggcgatttc aaatttcagt tctctgcaga ggtcatccac gtgcttgcgt
     5581 ctctggcgac gagtttgctt caggaagttt tcaatctcgc gatcaaagga tccctccgat
     5641 tcttcagatc tgtgtccctt gtaggcgatt cgaatgagag agagatcatc catgatttct
     5701 cctgtgcgac gcagagtgtc ttccagtgcg tttaaatctc cattcgcgtc cgctaccagt
     5761 cgaagaaaca aagtttcgtc tgtattgatt ccggcggaat ccgccaggcg aagatcgtca
     5821 cggccgtccg agccgcaaat caggacgtcc cccggtagaa ggcgcatcgt gaacacgcga
     5881 gcggaagtcg cctgcatggg cgatccaagt ttaaaacacg attgaccgtt agccgcaaat
     5941 gtaacctggc cttcgcgaaa caggacaggt gaaggatgct ctgcattgat gaaataagtg
     6001 caaccagttt gcccatcgag aagcccaatg atagcgctga tcatcatgga cccttcgaaa
     6061 ctctcgaaaa ctccctgcag ttccttctca ggcgattgag ccagctttct ggaaacacat
     6121 cgtgcgcctg acgcgaaact ttcgttctct gcaagtatgc gtgcatgacc gcaccaagta
     6181 caagcgcccc gccgacgccc tgcatggatt ttcccattgc gtcagagttc atgaagaaca
     6241 cgtagtctct ttcctgaagt cgcacatgat cgagcgtgac actgtctccg ccaatttgtt
     6301 ctttgaaatg cctgaactca aactttttga attgtgagat aagcgccgct gcttccaccg
     6361 aatgagtctg caatcctgca cgcaagaatg gtttcagcag cagagaagta agatagtagt
     6421 ctccatcctg cttttccttc acagtgcgca cttcgctcaa ggtagcgttg agctgctctg
     6481 tgcgaatttg aactttctct tcgagagacg tggcgtaaga ctccagctgt ccccgcgcat
     6541 tgcgaaggct tcgcgtgacc cggttgaagt ccgtggaaag cgctcctatt tcatctgtag
     6601 atgccatggt catttttgcc gtcaggtctc ccgatgcgac acggccaagc aaacgtgtca
     6661 gatacaaaac aaattgattc agatacgttg caaaaacgaa cgaaagcgca gcgacaagaa
     6721 ccactgcctg ggcggcggca gccactgtca tgagtctaag tttcgaaaca tcaatgtatc
     6781 caatggagag cggcgtagag ggtgtatcca aagaagacca gcaacagcca gaccccggaa
     6841 aacatagttc cgagagtttt ccggaaaaga gaaacgatca ccagatcctg acggcgaaga
     6901 aatactccgt gaaagattcg attgctgcga atcaattccg caagagagag ctgcatggcg
     6961 aggtagtgcg acactgcgtt gattggcgca agaaatatga acacaaaaac atagggcaga
     7021 aactctcgca tcgacagtgg ttcgagcaaa tggttcgtta gaacaacaac agcgatgccg
     7081 cccgcccact gtatgttcac cccgatggtt tcccagaacg gatagttcaa aaggcgccgg
     7141 agcaaattgc gtcgttgccg tccatgaacc tggccctcaa tgcacgcatt gatatccggt
     7201 gcaagcacgc gccagcggag aaccgggtaa agcatactcg cgaggactcc cacggtcgcg
     7261 ctgacgagaa agataatgag cttctggccg ctaaaacccg cttgaaagat tacgaacagg
     7321 attccgagcg gaacaccgag tagatacgtg actgcttcga gatttgcggt gagtcttgtc
     7381 aaaaatttcc gagggtttaa catgaggagt ctcagttgct cgggctgctt gtgtgcaggg
     7441 tctttgtggt cggtagcccg tggcgttaag cgttatgtgg tcagttcttt tctttctgtc
     7501 gactattatc tctgtattct cctcaatctg tgaaagatta tccttcccac actgtccacg
     7561 ccgcattttg tcgcaggttt atttgttttt cagtcacgtt cgtcaggttt gaggatgccg
     7621 gttcattgtt ttctttcaaa gacgctcaag cttcagtttg tatttcttga tagcgatcag
     7681 gttcgtgcta atcaactttt cctgataccg gtacgccatt tccgctccca taggaaccag
     7741 aatcgattgc gattgctttc tgcatttgct ttccagtccc gtgagatgcc ctataagagc
     7801 attgagtcta tgcagtcctt ctcctttcgc atgctcggga agcgggttca tgatttcaat
     7861 ttcttccgga agcacgttgg atccaaacac ctgttcgatc tcttcttcaa tcgcaattcg
     7921 atttgcatcg attcgccaga ggatgaaacg attcgcaagg cccatcaaat tgaatagaat
     7981 gctcacgccc atcaccagct gcaccagatt cgaaatgggg atgagatcca tcaggcgagg
     8041 caagtagcgg tcgaagatct ctgcaccctg gttgttataa aagtcaagag cacccgccgg
     8101 aagattgagt gctgtttcat ttaccactgt gcggttgtag ttgatgaaat tcggatagag
     8161 ttcactcatt acttgaagca ttcccacaat ttcggatctc ctcgcttcct ggtttgtcac
     8221 aagcaatgta ttgacgctta acaccggttt gtcgacgggg ggaatgtttc gcactggatc
     8281 gtacaatccc tgatgcatgg tgcgtgcctg cagatgagcc aggttcatcg tgacgctttc
     8341 cagtccgcga aggcccgcga tttgcagtcc ttctacaaga agtgcctttt tgatgaattc
     8401 tgatttttcg ctgattacga atacgcccag gtccagcttt ccttctttaa tcaaacgcag
     8461 ttgttcatcg gaaggatgaa agctcagctt cacacccagg gacttcatgg atggcatgcc
     8521 aaaaatactt tcagagaggt aggcagttcc acttcccaca gggcctccac caattcgcaa
     8581 tccgcgcagg tctgcgaaag aggtaagtcg atccgcgccg cgtcccagga ggaaca
//
LOCUS       DAOQJL010000100         8595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4412_length_8595_cov_4.194145, whole genome shotgun sequence.
ACCESSION   DAOQJL010000100 DAOQJL010000000
VERSION     DAOQJL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4412_length_8595_cov_4.194145"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLG78_06235"
     CDS             complement(<1..373)
                     /locus_tag="PLG78_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HNE22743.1"
                     /translation="MASQAEARMRKLEAEIRRHQNLYYVQHKPLISDRQFDRLLEELS
                     SLEKENPGLASPDSPTRLVGSDLDNSFPKFKHTIPVLSLANTYSTEEAVAWAEKTCEK
                     KETILIEWKVDGATLVLYYEKG"
     gene            complement(377..802)
                     /locus_tag="PLG78_06240"
     CDS             complement(377..802)
                     /locus_tag="PLG78_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE22744.1"
                     /translation="MGLKITNQRLLVAESIFRHPSHFTVDSLAESLKDRRDEISRATI
                     YRIVSILVESGQLTEHNFAQGVRYYEHIPRHEHHDHIVCQDCGRIEEFLVPEIEKVQQ
                     QIAEQHGFALEAHALNLYGRCAELKTKGSCARRLEKTPQ"
     gene            complement(873..1760)
                     /gene="thiL"
                     /locus_tag="PLG78_06245"
     CDS             complement(873..1760)
                     /gene="thiL"
                     /locus_tag="PLG78_06245"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNE22745.1"
                     /translation="MLSESQIIELFQKDSYLLDDCAAIPTNGEINLVSTDSLSEGTHF
                     RRDWSSPEDLAIKLLHVNISDLHSSGAKPSWCFLNLGLPSIEDDFIRRFARAFREERM
                     ACGIDLLGGDTFRSSELQLQLTMGGYAKRHVMRSGGRPGDVLYVTGTFGLSLAGYLHL
                     SGQVDAGGMASLAVGRHLRPRSHPYVDLTHVHAAMDVSDGIAQDSARLAKASRVRLEI
                     DLEKIPVAAGLKITAEEAVVSGEELELLFLADANFSDFLAVPIGVARSGEGCVFMRQG
                     DPVEIETGFSHFHAVDSSD"
     gene            complement(1762..2703)
                     /locus_tag="PLG78_06250"
     CDS             complement(1762..2703)
                     /locus_tag="PLG78_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22746.1"
                     /translation="MREFPVAVGHRLSEAEELLLRYWLESYAEGRWRSDSQMPSSPPA
                     DLSTLRFLPRADIRSVEEILSWFLPVVVFAGKASDPMPFISAGAVAVISLDDRNPLIL
                     PPAELPPRSSVAFVISDDPMFRLRMRQILRFAGREPRMDFSATEDIAQVLTHIQEWPE
                     IIVVDLDSSRVDVVEFIYRTEKILRERPAMRTRNQMMIVKDFAKPGLDPVKLRPVLSP
                     HAKRIFHPLEAMLAILESMVFYSGEVEPLADEHPRSLREMLYGDDFAPPGRLSESFLA
                     GLKSRGTALSRSSQFILFAQHLKDRLIETGSGSLAMK"
     gene            2748..4076
                     /locus_tag="PLG78_06255"
     CDS             2748..4076
                     /locus_tag="PLG78_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE22747.1"
                     /translation="MRKTWVCLLPVLASCFLFRPKNGHPEALKVELEDGGRIKTYSLR
                     YNPEGQNAARPLPGRSGRSIYTGLDVLAESDFALLREKRFALLTNSTGLNRALEPGID
                     LFLANGIRPEILFEPEHGIFAAEDAAGSEGVRTDSRTGLRIFSLYSSQKKPPSSLLLG
                     LDYLVVDIQNLPVRCYTYITTLSYLLEAAAEAGTPVLILDRPNPFGFIRARGPYPREE
                     LRSFVGYAPVPFLYSMTVGEYGHFVAHELLPNLKLSVIRVQGYVRADADAAASNSWIN
                     PSPNIPSFESALVYAGVVFFEGTNVSLGRGTTRPFIYVGAPWMHGPELATAMRSLKLP
                     GVQFSNVAFTPSSSLYTGVHCYGVQIHPTSLDFDPIRTGYELMRAVYRLHPGKLTFVW
                     SGNSYGTDRLWGSDSFRMGVINNWDYETFEKTWIRDSESFEEWIAAYRLY"
     gene            complement(4125..4667)
                     /locus_tag="PLG78_06260"
     CDS             complement(4125..4667)
                     /locus_tag="PLG78_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNE22748.1"
                     /translation="MKQSYLAVLAIAAMLSAPLFADEPHKGACKKDVQTYCKDVQPGE
                     GRIMNCLAENKDKVSAECKKNIGNFVNGFKQACGADEEKFCAKVERGQGRRLKCLNEN
                     SANLSPSCKAKLDEFKASHQAKHEERKAFMDACKADAKAQCKDVKPGQGRIISCLREK
                     ESSLSSACKAELAKVPASVQ"
     gene            complement(4725..6878)
                     /locus_tag="PLG78_06265"
     CDS             complement(4725..6878)
                     /locus_tag="PLG78_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE22749.1"
                     /translation="MRPWLWCFVIGMAVFQPAFAQTASIENSRDLVSLMGDWKFFPGD
                     RTEFAGATFDDSAWLSMRLPQNWHRAGIKYDGIAWFRRKIFVGEGMRDHTIGIVLPLR
                     EGWHEVYVNGRRIDDGASLEMSAPGAHPTRSFPVVIPSSFILFGQENTIAIRSRSYGG
                     VGGFISRTMYAGAMAAVERRYSLSVMWNTVLVTLFFLTGFTHFITFLSRRKEEYYLYF
                     AIFSISIGFIVASIDNLTHFVSDSFLLQHSSVHLPLIFAPSVLVLFAHSLFGRGRRAV
                     TIFSFVWSGLLLLAFFYSVSGPVGYGRYIRYLFPWILCSCALVILYCGTIAVMSIQEG
                     VFTGRIIGLGFLLFAVAVANDILSYLTIIHTPNIVEEGFFCFVVGMSASLSLKYARLQ
                     DSLHNLNRRLHDNIRALQSARSSIEDSEKKYRELVESTNEIIFSLDAGGSITMINQAV
                     GQHLGIPAQELIGQDFQALVYYAGTDSKNSLARHTVRQKLRELLDTRKEVLFRTDFFT
                     RTREPRELIVRLQYVPHNNSYTISGTASVESADELRAVCISERQVYVFGNYVYISDAI
                     SQRVFLGMLKYTDHDSAYAVKVCLREMIINSIEHGNLGITFEEKTEAQNEGTYFSLLL
                     SRQTDPVYGQRNVRLVYSLNEKRALFRITDQGDGFDHQSVLDRDIKSPEFFSLMHGRG
                     IAMARLEFDTIRYNEKGNSVLLIRTFSKHPSMKNV"
     gene            6978..7805
                     /locus_tag="PLG78_06270"
     CDS             6978..7805
                     /locus_tag="PLG78_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22750.1"
                     /translation="MDNVYQSPSLTPPPSGVDGAKFDLDRALNLGWQSFKGQIGLGVA
                     YTIVLALLSVLAVLSCFGILFALPHLAASSAIMGLLMVRGKPELGDMFRPFRAFGAVL
                     GAWLLITIVTQLIAMIFTIPYYAVLLQGVDWSQIQDASGGEAFKEIMQQFQKQQQTFS
                     VAKLGTNLISLLNLPVSLYIGARLMLTYPLIIERGIPPMEAIRQSLAVTRPFQWPLMG
                     FGFITGVIAGLGILGCCVGILFTVPLAFTMHGAAIYQLLGEDTGAPIPGTNTTQTSI"
     gene            complement(7827..8516)
                     /locus_tag="PLG78_06275"
     CDS             complement(7827..8516)
                     /locus_tag="PLG78_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22751.1"
                     /translation="MTFEVVDLHRSYGSRAILRGVNFILNQGELVCLMGENGAGKTTF
                     IDTVLSRVAPVRGEIRFHDQVLTTTEDRLHFISRTAVLGHDAGVFPDLSAEENLRFFE
                     RAYHGSLTGEGKERISELLSRAGLYSRRHDFVRTFSRGMRQKIGICRAVLHFPEVVFL
                     DEPITALDSAGVDFFFEILSDIRRTAGVFVVTHDPSVFRGEADGFILLAQGVFSPRAK
                     SDLNLKAYQRA"
BASE COUNT         2192 a         2239 c         2081 g         2083 t
ORIGIN      
        1 ttcccttctc atagtagagc acaagtgtgg caccatcgac tttccattcg atgagaatgg
       61 tttccttttt ctcgcaggtc ttctctgccc acgcaactgc ttcctctgtt gaataggtat
      121 tcgccagaga gagtactgga attgtatgct tgaacttcgg gaaagagtta tccagatcac
      181 ttccaaccag gcgagtgggt gaatcaggcg aagcaagtcc gggattttct ttttcgagag
      241 aactgagctc ttcgagcaac cgatcaaact gtctatctga gatgagaggt ttatgctgga
      301 cgtagtacaa attctggtgg cgacgaattt ccgcctccag tttgcgcatg cgcgcttctg
      361 cttgtgaggc catcttctac tgcggagttt tttcaagacg acgagcacag gagcccttcg
      421 tcttgagttc tgcacatctg ccgtacagat tcagagcatg agcttccaga gcgaatccat
      481 gttgttcagc aatctgctgt tgcacttttt caatttctgg aaccaggaat tcttcaattc
      541 taccgcaatc ctgacacaca atgtgatcat ggtgctcatg tctgggaatg tgttcgtagt
      601 atcgcacacc ctgtgcgaaa ttatgctctg tgagttgacc ggattctaca agaatagaaa
      661 cgattctgta aatggtggcg cgcgaaatct cgtctcttct gtccttcaga gactccgcga
      721 gcgagtctac cgtgaagtga gatggatgtc tgaaaataga ctccgctacc aatagccttt
      781 gattggtaat ctttagcccc atctgcttga ggtattccga gaagatggaa agttctttgt
      841 gaacgtcttt gaccacatcc cttctttacg gatcagtcag aggagtcaac cgcatgaaaa
      901 tgagaaaacc cggtttcgat ttcgactgga tcgccttgcc gcatgaatac gcagccttcg
      961 cctgaacgtg ccaccccaat gggaacggcc aaaaaatcgc tgaaattcgc gtccgccagg
     1021 aacaaaagct ccagctcttc cccgctcaca acagcctctt ccgcggtgat ttttagcccg
     1081 gctgcaacgg gaatcttctc cagatcgatc tccaggcgaa ccctggacgc ttttgcaagc
     1141 ctggcgctat cctgtgcgat tccatccgaa acatccatgg cggcgtgcac atgagtcaag
     1201 tccacatacg ggtgagacct gggcctgagg tggcgcccga cagccagaga cgccatccca
     1261 cccgcatcca cctgaccgct cagatgaaga tatccggcaa gcgacagacc aaacgtcccg
     1321 gtaacataca aaacgtcccc cggcctaccg ccagaccgca taacgtgccg tttggcgtag
     1381 ccacccatgg tcagttgcag ctgaagctca gacgatcgga atgtatctcc cccgagcaaa
     1441 tcaatcccgc aggccatcct ctcctcgcga aaggcacgcg cgaagcggcg gatgaaatcg
     1501 tcttcgatcg acggcaaccc cagattcagg aaacaccagg aaggctttgc accgctcgaa
     1561 tgcagatccg aaatattgac gtgaaggagt ttgatggcga gatcttccgg agaagaccag
     1621 tcccttctga aatgagtacc ttcgctcagg ctatctgttg aaacaaggtt gatttcaccg
     1681 ttagtcggaa ttgcggcaca atcgtccaac agatacgagt ctttttgaaa aagctctatg
     1741 atctgggatt cgctgagcat atcatttcat tgccaggctg ccggaacctg tttctataag
     1801 cctatctttg agatgctggg caaacagaat aaactgagac gatctggaca atgcggtccc
     1861 tcttgatttg agcccggcca gaaatgattc cgacagtctt ccgggcggtg caaaatcgtc
     1921 accgtacagc atttcgcgaa gcgatcttgg atgctcatcc gcgagtggtt ccacttctcc
     1981 actatagaac accatcgatt caagaatggc aagcattgcc tccagcggat gaaaaatcct
     2041 ctttgcatgc ggagaaagaa caggacgtag tttcaccgga tcaagccctg gcttcgcaaa
     2101 atcctttaca atcatcatct gattcctggt tcgcatagcc ggtcgttctc tcaaaatctt
     2161 ttccgtacgg taaataaatt caactacatc cactcgactt gaatccaaat ccacgactat
     2221 gatctccggc cattcctgta tatgagtcag aacctgtgca atgtcttccg ttgccgaaaa
     2281 atccatcctg ggttcgcggc ctgcgaaacg aagaatctgc cgcatgcgaa gacgaaacat
     2341 gggatcgtct gaaatcacaa atgcaaccga agatctaggc ggaagctcag ccggaggaag
     2401 aatcagtgga ttgcgatcgt ccaatgatat gactgcaacc gcaccggcag aaatgaaagg
     2461 catggggtcg gacgctttgc ctgcaaaaac aaccacgggc aggaaccagg aaagaatctc
     2521 ttctacggat ctgatgtctg ccctcggaag aaagcgaagg gtggacaggt ccgcgggtgg
     2581 agaggatggc atctgggaat cgctgcgcca tctgccttcc gcataacttt ccagccagta
     2641 tcgcagcaga agctcctccg cctcggaaag gcgatgcccg acagcaacgg gaaattcgcg
     2701 cataaatcag aattgcaact cgatgtgcac cgtcaacaca aggcacaatg cgaaaaacct
     2761 gggtatgcct gctgccggtc ctggcatcct gttttttgtt tcgcccgaaa aacggccatc
     2821 cagaagcctt aaaggtagaa ctggaagatg gcggtcgcat taagacgtat tcactgagat
     2881 acaatccgga aggacaaaac gctgcacgcc cccttccagg cagatccgga agatccatct
     2941 acactggtct ggacgtactg gcagaatcgg actttgcact tctgcgcgag aaaagattcg
     3001 cgctgcttac caattcaacc gggctcaatc gcgctctgga acccggaatc gatctgttcc
     3061 ttgcaaacgg aatcaggcct gaaattctgt tcgagccgga gcacggcatc ttcgcggcag
     3121 aagacgcggc aggaagcgaa ggagtccgca cagattctcg cacaggactt cgaatcttca
     3181 gtctctatag cagccagaaa aagccaccga gttctctatt gctcgggctt gattacctgg
     3241 tggtggacat acagaatctt ccggttcgat gctatacata cattactact ttgagttact
     3301 tgcttgaggc agcggcggag gctggaactc ccgtattgat tctggaccga ccaaatccat
     3361 ttggattcat acgcgcgcga ggcccgtatc cgcgcgaaga gcttcgtagc tttgttggat
     3421 atgcaccagt tccatttctc tattctatga ctgtgggaga atacggacat tttgtagcgc
     3481 acgaacttct tccaaatctg aagctatccg tgatccgagt gcagggctac gtgcgcgcgg
     3541 acgcagacgc ggcagccagc aattcctgga tcaatccatc gcctaacatc ccgtcattcg
     3601 aaagcgcgct ggtctatgcg ggagtcgtgt tttttgaagg aactaacgta agcctgggac
     3661 gcggaactac gcggcccttc atttatgtag gagctccgtg gatgcatggt ccagaactag
     3721 cgacggctat gcgatctctg aaattgcccg gagtgcaatt ttcaaacgtg gcgttcacgc
     3781 cctcgagttc tctgtatacc ggtgtgcatt gctacggagt acaaatccat ccaacctctc
     3841 tggacttcga tccaattcgg accggttacg aattgatgcg agcagtctac agactgcatc
     3901 ccgggaaatt gacgttcgtg tggtccggca attcgtatgg aacagatcgg ttgtggggaa
     3961 gtgactcgtt tagaatgggc gtaatcaaca actgggacta tgagacgttt gagaaaacct
     4021 ggattcgaga tagcgaatcg tttgaggaat ggattgccgc ctatcgtttg tattgagaga
     4081 ccagcccgat gggctggcct tccgtctggt tctttctggt tcttctactg aacagaagcg
     4141 ggaaccttag caagttccgc cttgcaggct gaactcagac tgctctcttt ctcgcgaagg
     4201 cagctgatga tccttccttg accgggtttc acatctttgc actgagcttt cgcatccgct
     4261 ttgcatgcat ccatgaatgc tttgcgttct tcgtgctttg cctgatgact cgctttgaat
     4321 tcgtctaact ttgccttaca tgaaggactc aagttcgcgc tgttttcatt gaggcatttc
     4381 agacgacgac cttgaccgcg ttccactttt gcgcagaact tttcctcatc cgctccgcat
     4441 gcttgcttaa aaccatttac aaaattacca atgttcttct tacattcggc agatactttg
     4501 tccttatttt ccgccaggca attcatgatt ctaccttcgc ccggttgaac gtctttgcaa
     4561 tatgtttgaa cgtctttctt acatgcacct ttgtgcggct cgtctgcaaa aagaggcgca
     4621 gagagcatgg ctgcaattgc caggacagct aaataacttt gtttcatgga gtttcctcgt
     4681 aggagatttc tacaggcatt agatcggacc cgctacggca gggttcaaac atttttcatg
     4741 gaaggatgct ttgaaaatgt gcgaatcagg agaacgctgt ttcccttttc gttgtagcga
     4801 atcgtgtcaa attccaaacg cgccatggcg attccacgac cgtgcatgag actaaagaac
     4861 tcggggctct tgatatcacg atcaagaacg ctctggtggt caaaaccgtc cccctgatcc
     4921 gtgattctaa aaagtgcgcg cttttcgttc agggaataga ccagcctgac gttacgttga
     4981 ccatacacag ggtctgtttg cctggacaaa agaagactga agtaagtccc ttcattttgc
     5041 gcttctgtct tttcctcgaa ggtaatcccc agatttccgt gttcaatgga gttgatgatc
     5101 atttcacgga gacaaacctt tacagcgtat gctgaatcat gatccgtgta cttcagcatg
     5161 cccagaaaga ccctctgact gatggcatca gaaatataca cataattccc gaacacgtat
     5221 acctgccttt ctgagataca cacggcgcgc agctcgtctg ccgactccac ggaggctgtt
     5281 ccggatatcg tataactatt gttatgtgga acatattgca ggcgaacaat cagctccctc
     5341 ggttctctag tacgcgtaaa gaaatcagtg cgaaataaaa cttccttgcg cgtatccagc
     5401 aactcgcgta gcttctgacg cactgtgtgc cttgcgaggg aattcttact gtcagttcca
     5461 gcatagtaca ccagagcctg gaaatcctgg ccgatcagtt cctgggcggg gatgcccagg
     5521 tgctggccga ccgcttgatt gatcatggta atgctgccgc ctgcatccag ggaaaaaatg
     5581 atttcgtttg tgctttcgac aagttcgcga tacttctttt ctgaatcttc gatactggat
     5641 ctcgcggatt gaagcgcgcg aatattgtcg tgcaggcgcc tgttcaagtt atgaaggcta
     5701 tcctgtaacc tggcatattt gagagacaga gaagcggaca tccccactac gaagcaaaaa
     5761 aatccctctt ccactatgtt gggagtatgg atgatggtga gataagacag aatgtcattg
     5821 gccacagcta ctgcaaatag tagaaatccc agtccaatga tgcgaccggt aaaaacgcct
     5881 tcctgaatac tcatgactgc gatggttccg cagtacagaa tcaccagagc gcaactgcat
     5941 agaatccatg gaaacaggta ccgaatgtaa cgtccgtatc caactggacc agaaaccgaa
     6001 taaaagaatg cgagaagcaa gagtccggac catacgaaag agaaaatggt aacggctcga
     6061 cgccctcggc caaatagact gtgcgcaaac aatacaagta cagaaggcgc aaagatcaaa
     6121 ggcaggtgaa cgcttgagtg ctgaagcagg aaggaatccg acacaaaatg agttagattg
     6181 tcaatcgaag ctacaatgaa cccgatcgaa atggagaata ttgcgaaata gaggtagtat
     6241 tcctcctttc tcctgcttaa gaatgttatg aaatgagtaa atcctgtgag gaagaagagt
     6301 gtcacaagaa ccgtattcca catgacagaa agcgaataac gtctctccac tgcagccata
     6361 gcacccgcgt acatagtacg cgaaataaaa ccacctacgc caccatagct tcgcgatcga
     6421 attgcgattg tgttttcctg tccgaacaaa ataaaagagg atggaattac tacgggaaac
     6481 gatctagtcg ggtgcgcacc cggcgcggac atttcgagag aggcaccatc gtctatgcga
     6541 cgtccgttca catatacttc atgccagcct tcgcgcaagg gtaggacaat tccgatggta
     6601 tggtcacgca tcccttcacc gacgaaaatc tttctgcgaa accatgcaat cccgtcgtat
     6661 ttgatgcctg cacgatgcca gttctgtggc aacctcatgc tcagccaggc agagtcatca
     6721 aaggtggctc ccgcgaactc tgtgcgatca cccggaaaga acttccaatc acccatgagg
     6781 ctgaccaggt cacggctgtt ttcaatcgat gcggtttgtg caaaggccgg ctgaaacacg
     6841 gccatgccga tcacaaaaca ccagagccac ggccgcatga tgtcctcctg ttgcactgaa
     6901 atcgggttgg gtcaagccaa atagaccact tgaacaggat ttgcttgcaa ctcctggccc
     6961 ggactccccg cttgctcatg gataacgttt accaatcacc gtcgctgact cctccgcctt
     7021 ccggagtaga cggagcaaaa ttcgatctcg atcgcgcgct caatctgggc tggcagtcct
     7081 ttaaaggcca gatcggtctt ggagtagcat atacaatcgt gctggcactg ctgagtgttc
     7141 tcgcagtgct ttcatgtttt ggcattctgt ttgcattgcc tcacctggcc gcttcttctg
     7201 caatcatggg actgctgatg gttcgcggga aaccagaact gggagatatg tttcgcccat
     7261 ttcgcgcatt cggtgcagta ctcggcgcat ggcttctgat cacgattgta acgcaactga
     7321 tcgcgatgat tttcacgatt ccatactacg cggttttgct ccagggcgta gattggagcc
     7381 agattcaaga tgcttcggga ggcgaagcat tcaaagaaat catgcaacaa tttcaaaagc
     7441 aacagcagac cttttcagta gcaaaacttg ggaccaatct aatctctctt ctgaacctgc
     7501 ctgtttcatt gtacattggt gcaaggctca tgctgaccta tcctttgatc attgaacgcg
     7561 gaattccacc catggaagca atcaggcaaa gccttgccgt tacgcggcca tttcagtggc
     7621 cgctgatggg tttcggattc attactggtg tgatcgcagg gcttggtatt ctgggttgct
     7681 gcgttggtat tttgttcact gtgcccctcg cattcaccat gcacggagcc gccatctatc
     7741 agctgcttgg cgaagataca ggcgctccca tcccgggcac taacacgacg cagacttcca
     7801 tctaggagat cattcgccgc ataacgtcac gcacgctggt aggctttcaa attcagatcg
     7861 ctcttcgcgc gcggggaaaa tactccctgc gcgagaagaa tgaatccatc cgcctcaccg
     7921 cgaaatacag aagggtcgtg ggtcacaaca aagacccccg cagtccgtct gatatcagac
     7981 agtatttcaa aaaagaaatc cactcccgct gaatcaaggg cagtgattgg ctcgtccaga
     8041 aatacaacct cgggaaaatg aaggaccgcg cggcatattc cgatcttctg cctcattccg
     8101 cgagaaaacg tacgaacaaa atcatgccgt ctggaataga gaccagcacg cgacaaaagc
     8161 tcgctgattc gttctttgcc ttcccccgtc aatgaaccat gatacgctcg ttcaaaaaaa
     8221 cgcagattct cctctgcaga aagatctggg aacacaccgg catcgtgacc tagaactgcc
     8281 gtgcgagaaa tgaaatgcag tctatcttct gtagtggtaa gaacctgatc atggaatcga
     8341 atttccccgc gaaccggagc cactctgctt agaaccgtgt cgatgaacgt ggtcttgccg
     8401 gctccatttt ctcccatcag gcaaacgagc tcgccttgat tgagtataaa atttacgccg
     8461 cgaaggatgg cgcgtgatcc ataactgcga tgcagatcaa caacttcaaa cgtcattctt
     8521 cttaaccagg cgaaacccgg aggaagacac aagggaggcg ccgagaaata aaatgagtac
     8581 ggtcagaatg aatgc
//
LOCUS       DAOQJL010000101         8587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4415_length_8587_cov_4.138537, whole genome shotgun sequence.
ACCESSION   DAOQJL010000101 DAOQJL010000000
VERSION     DAOQJL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_8587_cov_4.138537"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLG78_06280"
     CDS             <1..440
                     /locus_tag="PLG78_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE22752.1"
                     /translation="EMIETARILRRATAHSLVILDEVGRGTSTYDGLSIAWAIVEALS
                     DRRSLVLFATHYHELTALDQKPGIFNLTMDVREIDGKIHFLRKVREGAADRSYGIHVA
                     ELAGIPESVLRRAKEKLFELESRPGKKAKSVRLKPAETEPGLF"
     gene            complement(443..1327)
                     /locus_tag="PLG78_06285"
     CDS             complement(443..1327)
                     /locus_tag="PLG78_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22753.1"
                     /translation="MMRLRKALHKIIRFSPAILLGLSWFCIPRPAKTDVRWKPVLSLP
                     RDAAIMQLRTEGAQDLLVYSGVQDGLSEIGGATDLLMSSGWNLLTLKTADRDPDISKA
                     FFEFAGNRPGFRRKILMVPGRDLCAASGSMEMSSLSALVVFRAEDSTFPDNECTTRLS
                     AIRPDLPVLFLVSPGLPGMSFVLYQAVPSSSKEYKTDSNMSPNVKTAFFQGRLLTFLH
                     LQRFPISFVESRTTFKKGCMMLPSGPWVIAFVSGQRAEGYCPLFLKSSGELYRAVDVR
                     PNAGCDYGVTSESAIRCQ"
     gene            complement(1327..1992)
                     /locus_tag="PLG78_06290"
     CDS             complement(1327..1992)
                     /locus_tag="PLG78_06290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE22754.1"
                     /translation="MGVRTIFIGDVHGCVDELKEMIGVLGPTREDRVILLGDLINRGP
                     DSPGVVRFVFESGFESLMGNHEYEYLRHYTQIPVFKKIREGLGQELHSWLEGRPLFIE
                     TDAFLAVHAGLFPGKHPAETPEEVLLNIRTVRPEEGFDSDLNRNGDPAWHTYYKGTKP
                     VFYGHWARQGLTISHPTYGLDSGCVYGRALSAYVLETGELIQVPAKNVYYRKPYDSSP
                     ESG"
     gene            complement(1983..2876)
                     /gene="hemF"
                     /locus_tag="PLG78_06295"
     CDS             complement(1983..2876)
                     /gene="hemF"
                     /locus_tag="PLG78_06295"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNE22755.1"
                     /translation="MFESLVKFFYDLQDQITGALEQESGSSKFHEDTWTGEGWLAGGG
                     RTRVMESGTVLERGGVNVSDVHGLLPDDLARNMPGKAREFRAAGISLVLHPLNPHAPT
                     VHANFRIISRGKQEDPETIWFGGGADLTPMILYEEDATHFHSIWKKVCDRYPSMPYTK
                     LKEDCDRYFYLPHRKEGRGIGGIFYDYVDTAPEVMGQFTREAGSSFLDAYLPLLKKRS
                     SQSYTEEDREFQMWRRGRYVEFNLVYDRGTQFGLKTGGRIESILMSLPPIVRYKYDYR
                     PAPGSRYEATMQALSNPRTWV"
     gene            complement(2869..3864)
                     /locus_tag="PLG78_06300"
     CDS             complement(2869..3864)
                     /locus_tag="PLG78_06300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE22756.1"
                     /translation="MKVDLNTASDILLSGNIVAIPTETVYGLAADALNPDAVLQIFEA
                     KKRPRFNPLICHLPDVADVKKYCHPPACLEVLASLWPGPLTILMPHNGSIPSLVTAGS
                     SLCAFRVPSHPIALELLKKVRRPLAAPSANKSGRVSPVNAEMVEREFRGSLPVLDGGQ
                     CSVGLESTIVLANEDSVSILRNGKYTALDFENLGLKVEPPAEDIRAPGQMLRHYAPEV
                     PLILLTVERKRVESEAMHVDAKSKIAWLGFRGSKPPFDAQVFELSQSGDLHEAARNLF
                     FYFDRMARERFDYIVAELVPEEGIGLAINDRLRRAADMEGNVQNERLSAWRIMHV"
     gene            complement(3861..5108)
                     /locus_tag="PLG78_06305"
     CDS             complement(3861..5108)
                     /locus_tag="PLG78_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE22757.1"
                     /translation="MSSFRPGSTVSQLPVHRLPGGERLLFMRNNSYITNIQILTNVGS
                     MAEDPQIHGMAHILEHMFFKGSNKFRGGTAISRAANDIGGKLNAYTTYDHTVFYISVL
                     GDEFEQGFEILSDMYRNPLFPPDEFKKELNPILSEMREREDDPESFLMESALRAYLGS
                     SYHPVIGNADTVKAATVENMHRFKSRYYAGNNCMISIVGGIEEERVHKAVAEHFTDLR
                     HGESAVTPSFEYKTGGIELTKPGIQEAHFSLFYPALGPDHPDRYKQDLMNYLLGGNES
                     GLLFERIREELGLSSYGIYSYVMRTPGHAVLGISGGIAPDELHLMEKEVKSCIARICE
                     SKLEEHRLHRARASLRTSIASYAETSSGLNGMIGLPALRGDTSHPVEKALSEIEKITL
                     EDVHEMAQKTFSGPQFRAVLTPA"
     gene            5187..5570
                     /locus_tag="PLG78_06310"
     CDS             5187..5570
                     /locus_tag="PLG78_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNE22758.1"
                     /translation="MRRIYLSASALLGLFSVMLGAFATHALKGTIPDADLAIFETAAR
                     YQMYHAIAMLALGLAMDRVGKSGRAVLGLWLFGTIVFSGSLYAIVLTGVRAFGAVAPI
                     GGLSLMAGWAFLFVSAVRHEGFPPR"
     gene            5589..7445
                     /locus_tag="PLG78_06315"
     CDS             5589..7445
                     /locus_tag="PLG78_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22759.1"
                     /translation="MGRLILLLLLPGALFSQGLYKQPSEIIDPAQVDDLEKSALGLNP
                     DCEAILTRANNLLSSYYSQFIADKRIQDERRNPSFAAETLSENTVRLIALARSKEPGR
                     EYFLGAQPYLYRLHRLKGVCYHKTEEHTKALNEYAMAFRYTALDPAYQDNPDKNELSA
                     IYLRMAQNFGDKERARQEQDPALRSDSTGLRDLVDRFIQTSGALEAAEKQISVEEARV
                     VRGKGGNPAAARAKRDSLKQQVDSLRSQVEGVRKGSYRTFALRCQKENGELAFTMANI
                     VQELEQKNQEVERILNRSSFYRGIGNELGEDRTSYSGFTGYGILLEFANKIDPSNLLY
                     ISKLADEHRKNRRRDLAIRFYEDYFAKASALAQKPADFADNLLKLAGVFTDSQNYIRA
                     ADMLEQYDALVSSPAAKLQLADIHYYRTGRTARAKELYDAYMAARPATAGTLKDRVES
                     GLVAFRVQKNLASIARRNLRTGEEMGALAAAENVFRSLEKEEADARKVEEDLRAKLFE
                     IKRRLLGREDEDLQREYYRLQRIELAQAMEQTGYVHTRVDSMNIGQVLERQAYLAERQ
                     RNFDLAREKYRELVVRGTGPEQSRARENLERLRLSLADGLLRPPSLSPDFER"
     gene            7491..7889
                     /locus_tag="PLG78_06320"
     CDS             7491..7889
                     /locus_tag="PLG78_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE22760.1"
                     /translation="MAGRNDSMNSVIGPGSIFEGKFYISGSLKIDGKFEGEIKTEDAL
                     VVGETGKIKTNITAKNVVVAGTMIGNIQAESEVRLEKSGKLLGDITAPMLNIQPGVIA
                     KGTITITGGQKKDVRKVIEESYGGLKISEK"
     gene            7970..>8587
                     /locus_tag="PLG78_06325"
     CDS             7970..>8587
                     /locus_tag="PLG78_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE22761.1"
                     /translation="MYHRRFKNRPGRKILNLQGRFSLIYTGGGNFVYTLPGKDTPVVR
                     RLDLSSWKYRVSLSAGAFALFLFSGFGIWTSQGTASSSGVVVEEARISPAELEKRSEA
                     LKEKIIKNNAPHVDQSFEYTVRPGDTLSEIAAVYKVLPASILSASGVKRADEVKPGQK
                     LTIPNRPGLMYRMKPGDTLAAVAQFYTVSLEDIQNDNPELRDMDLL"
BASE COUNT         2105 a         2171 c         2211 g         2100 t
ORIGIN      
        1 tggagatgat tgaaactgcg cgaattctgc ggcgcgccac tgctcattca cttgtgattc
       61 tggacgaagt ggggcgcggt acatctacct atgatgggct ttcgattgca tgggcgattg
      121 tggaggctct ctccgatcgt cgttctctgg tcctgtttgc cactcactat cacgagttga
      181 ctgctctcga tcagaaaccc gggattttca atctcaccat ggatgttcga gaaatagacg
      241 gaaaaattca tttcttaaga aaagtgcgcg aaggtgcggc ggatcgatcc tatggaattc
      301 acgtggcaga gcttgctgga attccagaat cagtgttgcg tcgtgccaaa gagaagctat
      361 ttgaactcga atcgcgtcca ggaaaaaaag caaagagcgt tcgcctcaag ccggcggaga
      421 cggaaccagg attattctga attcactggc agcgaatcgc cgactcactt gtcacgccgt
      481 aatcgcaccc tgcattcggg cgcacgtcca ctgcgcggta gagctcgccg cttgatttaa
      541 gaaacaaggg acagtatcct tccgccctct gtccactgac aaaagcaatc acccatgggc
      601 ccgacggaag catcatacaa cctttcttga aggtagtgcg cgattccacg aaagaaattg
      661 gaaacctttg aagatgaaga aaagtgagca gtcgtccctg gaaaaaagca gtctttacgt
      721 taggcgacat attggagtct gttttgtatt ctttgctgga gcttggaact gcctgataca
      781 agacaaaaga cattccggga agcccggggg aaaccagaaa caaaacagga agatccgggc
      841 gaatggcaga aagtcgagta gtgcattcat tgtccggaaa agtcgaatct tccgcgcgaa
      901 agaccacgag cgcactgagg ctggacattt ccatggaccc ggaagcagcg cataaatctc
      961 ggcctggaac catgagaatc tttctgcgaa atccaggtct gtttcctgca aactcaaaga
     1021 atgcttttga aatatcagga tcgcggtcgg cagtcttcaa ggtcagcaga ttccagccgc
     1081 tgctcatcag taagtcggtg gcgcctccaa tttcagaaag tccgtcctgg actcccgagt
     1141 agaccaggag atcctgcgcg ccttcggtgc gcagttgcat gatcgccgca tcgcgcggca
     1201 gagaaaggac tggcttccat cgcacatctg ttttagccgg gcgaggaatg caaaaccagg
     1261 ataagcccaa cagaatggcg ggagagaacc gaatgatttt gtgaagcgct tttctgagtc
     1321 gcatcatcag cctgattccg gtgaagaatc gtatggtttt ctatagtata cgtttttggc
     1381 gggaacttga atcagctcgc ctgtctccag aacgtaggca gagagtgctc taccgtagac
     1441 gcatccagaa tcgaggccgt aggtggggtg agaaatagtc aaaccctgtc tggcccagtg
     1501 cccgtagaat accggtttgg ttcctttgta gtatgtatgc catgcagggt cgccgtttct
     1561 gttgagatca gaatcaaatc cttcttccgg gcgcacggtt cgaatgttga gcagtacttc
     1621 ttctggtgtt tcagccgggt gttttcctgg aaagagccca gcatgcacgg cgaggaatgc
     1681 atctgtttct atgaacagag gtctgccttc aagccaggag tgaagttcct gacccaggcc
     1741 ttcgcgtatt tttttgaaca ctggaatttg agtgtagtgt ctgagatatt cgtactcatg
     1801 atttcccatg aggctttcaa aaccgctctc aaatacgaag cgaaccactc ccgggctgtc
     1861 tggaccccgg ttgatcaagt caccgagcag aatgacacga tcctctcgag tcggcccgag
     1921 taccccgatc atttctttga gttcgtccac gcagccgtgg acatctccga tgaagattgt
     1981 tcttacaccc atgttcttgg attggacagt gcctgcatcg tagcttcgta tctgcttcct
     2041 ggtgcaggcc tgtagtcgta cttgtatcgg acgattggag gcagactcat gaggatggat
     2101 tcaattcttc cgcccgtttt gaggccgaac tgtgttcccc ggtcatatac taagttgaat
     2161 tccacataac gtccgcgtct ccacatttga aactcgcggt cttcttctgt gtaactttga
     2221 gaggatcttt tcttgaggag tggaagatac gcatccagaa acgagctgcc cgcttcgcgt
     2281 gtgaactggc ccatgacttc gggcgcagta tccacatagt catagaaaat gccaccaatc
     2341 ccacgacctt ccttgcggtg cgggagataa aaataacgat cgcaatcttc tttcagtttt
     2401 gtgtaaggca tggaggggta tctatcgcac acctttttcc agatactatg gaaatgcgtc
     2461 gcatcttctt catagagaat catgggggtg aggtccgccc cgccgccaaa ccatattgtt
     2521 tccgggtctt cctgctttcc gcggctgatg attctgaagt tcgcgtgcac tgtgggcgca
     2581 tgaggattca acggatgaag caccagagaa attcccgccg cacgaaactc gcgtgccttt
     2641 ccaggcatgt tgcgcgccag atcatccggg agaagtccgt gcacgtcgga aacattcacg
     2701 cctccgcgtt cgagaaccgt cccagattcc atgactctgg ttctaccgcc gcccgccagc
     2761 cagccttcgc ccgtccaggt gtcttcgtgg aacttcgaag atccggattc ctgttcgagc
     2821 gcacctgtga tttgatcctg taaatcatag aaaaatttca ccaggctctc aaacatgcat
     2881 gattctccag gcagacaaac gttcattctg cacattgcct tccatgtctg ctgcgcgtct
     2941 cagtctgtcg ttgatcgcaa gcccgattcc ttcttccggg acgagctctg cgacgatgta
     3001 atcgaagcgt tcccgggcca tgcggtcaaa gtaaaaaaat agattgcgcg ctgcttcgtg
     3061 cagatccccg ctttgcgaga gttcaaacac ctgagcgtca aacggaggtt tgcttccccg
     3121 aaaacccagc caggcgattt tgcttttcgc gtccacatgc attgcttctg actctactct
     3181 ctttcgttcc actgtaagca gaatgagcgg aacttcaggc gcatagtgcc gcagcatttg
     3241 tcctggcgca cggatgtcct cggcaggcgg ttccactttc agtcccaggt tttcaaaatc
     3301 aagagctgtg tacttgccat ttcgtaaaat cgaaacgctg tcttcatttg ccaggacaat
     3361 cgtagactca agtcctacgg aacactggcc tccgtcgagg actggaagcg atcctctaaa
     3421 ttctctttcc accatttctg cgttcaccgg gctcacgcgt ccgcttttgt tcgctgatgg
     3481 agcggccaga ggccttcgca cttttttaag tagctcaagc gcgatcggat gggaaggaac
     3541 tcgaaaagca catagactag agccagctgt tacaagagag ggaatagacc cgttatgcgg
     3601 cattagaatg gtcagaggac cgggccatag agacgccagt acttccaggc aagcaggtgg
     3661 gtggcagtat tttttaacgt ccgctacgtc aggcagatga cagatcaaag ggttgaacct
     3721 cggtctcttc tttgcttcga agatttgcag tactgcatct ggatttagag cgtctgctgc
     3781 cagtccatag accgtttcgg ttggaatggc aacgatattt ccagacaata gtatgtctga
     3841 tgctgtgttt agatcaacct tcaagccgga gtgagaacgg cccggaattg cggaccggag
     3901 aaagtttttt gagccatctc atgcacgtct tccagtgtga tcttttctat ttcagagagt
     3961 gctttttcta cagggtgaga agtgtcccct ctgagtgcag gcagtccaat cattccattg
     4021 agtccgcttg acgtttctgc ataggatgca atcgacgtgc gaagagaggc cctcgcgcga
     4081 tgaagtctgt gttcttccag tttggattcg cagatccgtg caatgcacga tttcacttct
     4141 ttttccatta ggtgcaattc gtccggcgcg attcctccag aaatgccaag tacagcatgc
     4201 ccgggtgtgc gcataacgta ggaataaatc ccatagcttg atagtcccag ttcttctctg
     4261 attcgttcaa agagtaagcc ggattcgttt ccacccagaa ggtaattcat caaatcttgc
     4321 ttgtatctgt ctggatgatc tggaccgagc gcgggataaa aaagggaaaa gtgtgcttct
     4381 tgaattccag gcttcgtgag ttctattcct cctgtcttgt attcaaagga aggagtgact
     4441 gcggattccc cgtgtctgag gtctgtgaaa tgctctgcga ctgctttgtg tactctttct
     4501 tcttcaatcc cgcccactat gctgatcatg cagttgtttc ctgcatagta tctggatttg
     4561 aatcgatgca tgttttctac agtggctgct ttgaccgtat ctgcgtttcc aatcacagga
     4621 tgatacgatg acccaagata cgcgcgaaga gcgctctcca tcaaaaagga ttctggatca
     4681 tcttctctct ctctcatttc agataggatg ggattgagtt ctttcttaaa ttcgtccggg
     4741 ggaaaaagag gatttctgta catgtcagac aggatttcga atccctgttc gaattcgtct
     4801 ccaagcacac taatatagaa gactgtgtga tcgtaggttg tatatgcgtt gagttttccg
     4861 ccaatgtcat tggccgcgcg cgagatcgca gtgcccccgc ggaatttgtt tgatcccttg
     4921 aaaaacatgt gctccagaat gtgagccatg ccatggattt gcggatcttc cgccatggag
     4981 cctacgtttg tcagaatctg gatgttcgtg atgtagctgt tgttcctcat gaacaacaaa
     5041 cgttctcctc cgggcagcct gtggactggc agctgggaga ctgtgcttcc gggtctaaat
     5101 gaagacatat tccataacat agaaagagcg cactcgtgtc acggcgaatt gttttgaccg
     5161 ccgccgggga tgattcgcac tgcggcatgc gcagaattta cctgtccgcc tccgccttac
     5221 tcggattatt ctcagtcatg ctgggagcgt ttgccactca tgcactcaag ggcaccatcc
     5281 ccgacgcaga cctggccatt ttcgaaacag ccgctcgcta ccagatgtat catgccatcg
     5341 ccatgctggc actgggactg gccatggacc gagtgggcaa atcgggtcgg gcggttctgg
     5401 ggctctggct ctttgggacg attgtctttt cgggaagcct ctatgccatc gttttgaccg
     5461 gggttcgggc ctttggggca gtggctccga ttggcgggct ttccctgatg gcaggctggg
     5521 ccttcttatt cgtatccgcc gtcagacacg aaggctttcc tcctcgctga tttggccgaa
     5581 actgtagtat gggtcgcctc atacttctat tgcttttgcc cggggccctg ttctctcagg
     5641 gtctctacaa acagccttct gaaatcatag accccgcgca ggtggacgac ctggaaaagt
     5701 cggctctggg actcaatcct gactgcgaag ccattctcac gcgcgcgaac aatttgcttt
     5761 cgtcctacta ttctcagttc attgcagaca aacgcatcca ggacgaacgc cgcaatccaa
     5821 gctttgccgc agagactttg agtgaaaaca cagtcaggtt gattgcgctg gctcgaagca
     5881 aggagccagg gcgagagtat ttcctcggtg cacagccata tctgtatcgg ttgcacagac
     5941 tcaaaggagt ctgctaccac aagacggaag aacacaccaa agcgctcaac gaatatgcca
     6001 tggcatttcg atacacggcg ctggatccgg cttaccagga caatccggac aagaatgaac
     6061 tatccgcgat ctatctacgc atggctcaga acttcggcga caaagagaga gccagacagg
     6121 aacaggatcc ggcgcttcga tccgactcca ctggtcttcg cgatctggta gatcgattca
     6181 ttcagacgtc aggcgctctt gaggctgcgg aaaaacagat ctcagtagaa gaggctcgtg
     6241 tggtcagagg gaagggcgga aatcccgcag ccgctcgcgc aaaaagagac tctctcaaac
     6301 aacaggtaga ctcacttcgc tctcaagtgg aaggagtcag aaaaggaagc tatcgcacat
     6361 tcgctcttcg ctgtcagaaa gaaaacggtg agcttgcctt cacaatggcg aatatcgttc
     6421 aggaactcga gcagaaaaac caggaagtgg aacgcattct aaatagatcg agtttctatc
     6481 gcggaattgg aaacgaactg ggagaggata gaacgtccta ttccggtttt accgggtatg
     6541 gaattctact cgagtttgca aacaagatcg atccttccaa cctcctttac atttctaaac
     6601 tcgcagacga gcacagaaag aatcgaagac gcgatcttgc cattcgattc tatgaagact
     6661 actttgcaaa agcatccgcg ctcgctcaaa agcctgcaga ctttgcagac aatctcttga
     6721 agctcgccgg agtattcact gattcacaga attacattcg cgccgctgac atgctggagc
     6781 aatatgatgc acttgtatcg agccccgcag ccaaactcca gctggcagac attcactact
     6841 atcgcacagg tcgaactgcg cgtgccaaag aactttacga cgcctacatg gctgcgcgcc
     6901 cggcaactgc aggcaccctg aaagatagag tggagtcagg actcgtcgca tttcgcgtac
     6961 aaaagaacct cgcgtcgatt gctcgaagaa atctcagaac aggcgaagag atgggggcac
     7021 ttgccgctgc tgagaatgtg tttcgatctc tggagaaaga agaggcggat gcgcgtaaag
     7081 tagaagaaga tttgcgcgcg aagctttttg aaatcaagag aaggcttctt ggaagggaag
     7141 acgaagacct tcagagggaa tactatcgtc tgcagaggat cgaactggct caggctatgg
     7201 agcagacagg atacgtgcac actcgggtgg actctatgaa cattggtcag gtactagaga
     7261 ggcaggcata cctcgcagaa agacagcgca actttgatct ggcccgggag aaatacagag
     7321 agctggttgt gcgcgggacc ggtcccgaac aatctcgcgc tcgcgaaaac ctggaaagac
     7381 tgcgcttgag tctcgcagac ggcctcctca gaccgccttc cttgtctccg gactttgaac
     7441 gatgatcttt tttagttgta agaaaaccgg caaaagggac aactgactcg atggcaggcc
     7501 gcaacgattc catgaacagc gtgatcggtc ccgggtcgat ctttgaggga aagttctaca
     7561 tttccggatc tctgaagatt gatgggaagt tcgagggcga aatcaaaaca gaagacgcac
     7621 tcgtagtcgg ggaaaccggc aagatcaaga ccaacatcac agcgaaaaac gtggtggtgg
     7681 ccggcacgat gatcggcaac atccaggcag aaagcgaagt aagacttgag aaatcaggca
     7741 agctgctcgg ggacatcacc gcccccatgc tcaatatcca gccaggcgtc atcgcaaagg
     7801 gaaccatcac catcacaggt gggcagaaga aagacgttcg taaggtcatt gaagaatctt
     7861 atggagggtt aaaaatctcc gaaaagtaag aggtggctaa tgtgacataa ttttttgctc
     7921 gcaaacttcc tccctctcgc cgattggtta aaaaggcccc ttcgactcca tgtatcatag
     7981 acgatttaaa aaccgcccgg gtcgaaaaat cctgaatctt cagggtagat tttcgctcat
     8041 ctatactggc gggggaaact tcgtctacac tctcccaggc aaagacactc cagtcgttcg
     8101 caggctcgat ctgagttcgt ggaagtaccg cgtcagtttg agcgcgggtg catttgcatt
     8161 atttcttttt tcaggctttg gaatctggac cagccaggga accgcgagtt ccagtggggt
     8221 agtggtagaa gaagcgcgca tttctcctgc tgaactcgag aagagatcag aagcgctcaa
     8281 agaaaagatc atcaagaaca acgctcctca tgtagatcag tctttcgagt atacggttcg
     8341 tcccggagat acgttatccg aaattgcagc tgtatacaaa gttcttccag caagtatcct
     8401 atctgcttcc ggtgtgaagc gcgcggatga agtaaagcca ggccagaagc tcaccattcc
     8461 caatcgtcct ggtctcatgt atcgcatgaa gccaggagac actctggctg cagtcgcgca
     8521 attttacact gtgagtctag aagacattca aaacgataat cccgagctgc gcgatatgga
     8581 cctgctt
//
LOCUS       DAOQJL010000102         8505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4478_length_8505_cov_4.722012, whole genome shotgun sequence.
ACCESSION   DAOQJL010000102 DAOQJL010000000
VERSION     DAOQJL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_8505_cov_4.722012"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLG78_06330"
     CDS             complement(<1..819)
                     /locus_tag="PLG78_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE22762.1"
                     /translation="MKRFFIPPDFNLAAAWFADATLPASLRTYQNIHELEITPADREM
                     LRSLRHNRLLFFTNHPSQAEPMIAYHIANVMGSRFHYMATRRAFDFLFGAVGKLFQSV
                     GAYSILPGVADRDSMRMTRQILSAPGGKLAIFPEGEPMCGENDSLMPFQPGFLKLGFA
                     ALQDARKTEPTANISVLPGFIKYVIKSSREQIIEHLTQSIGTIEKKLGLEAGHRNLLR
                     QFLMVGRVLLENSEKEYGITPPADADFDFRIGKLRHTILDRVAEMMKLKHYNTGA"
     gene            complement(860..3469)
                     /locus_tag="PLG78_06335"
     CDS             complement(860..3469)
                     /locus_tag="PLG78_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22763.1"
                     /translation="MNSGEFEHHLRTIANDDRDFLYPNWIASAQYFERLARSGESIFP
                     ALDPLLRIFYETHQVPVMQPAIRAVMIELLRENRTHEIENTFLHDDFKTWVIEALADL
                     ARSFDIGPAMPSIVLHFEASPFRAAEAIAMQIRKKRATVAQVCDLMMTEEFSRKSLVR
                     VLATTASSGIDITEHVSSYARLLTYQDSQKLAIQELPWFTGESKANIAIVEELGRIAN
                     DPNAESDVRERAAYFLADHYSTTDAEKFSSLLENESSFVRAGTAIFLAHTIPKSRGTR
                     RAEWIDKLTLYLLDQDSYVRSVALRQIDSMIQSGNKFEPSLDAIAKLAQAAKKQEDLI
                     PFLLHFASESKSLKSVIEKSLASPQSENAALILKALREPTVRRCSICSSLNRVESWNH
                     ELRTPAQIKKMRESGNLRFCTECENIYQFSYSSEVEDNHTSESFELERLHPAAALKML
                     QGEDAIQYKEQLPRLLQSLRSETSHVDEWIRSEAAWKLAEYLLSTEQWTEIDTLSLQN
                     QTCLKEITLAVASAVRSGVDAGHTLTTIRRAMQAEHCETRMNSAFVLSCMSLQGESNE
                     LPEILYSTDSCVLQGCLQALFAHASRDLPCPHLDRIAALTLHDDDRVRAFANYVVERA
                     KENGQKIPFLVPNLMKALRSKNARMKQDGMRFLRSEITQQNAAQILGEAVSLLKDSER
                     YGLVELIEACAEAGASLERYTGQFISDLQDSRGDLLRLLTVAARQNPSMIGRNATDAI
                     AECLNDSRLLPAASHLFEFLSNSKCDLTTAMPAIQSFIDRAVSTDSYTESLIQVLIRS
                     LAGLSDYESIMKLLSHRTTTIRLSVLYRIKDLASEGLQFRAILEPLQSLLKDPSAGVR
                     EMAEKVITQVSQS"
     gene            complement(3485..5092)
                     /locus_tag="PLG78_06340"
     CDS             complement(3485..5092)
                     /locus_tag="PLG78_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE22764.1"
                     /translation="MMRTLSLFIFTVRLLIISVFVYHGTKPLSAPRRSAARNKVTLWA
                     AKKIATFFIEMGGIYIKAAQFLSTVSNLFDSEFTEVFASVQDKITPRPYAQVRYRFLN
                     EFGSEPESLFQEFDHTAIAAASLGQVHLARTKDGRKVAVKFLHPGIESQIRQDIRALK
                     LAVNLILWVYPYLDFRPHLYEFSTMTLTEIDYENEAENMRRIRDNFANDPRVVVPSVV
                     DELSGGTVLTTEYIEGINISEIEELDARGIARREAAELLVETYVRMIFDDRFYHADPH
                     SGNIFVIPPDGVRPLRIGLVDFGATQSIPERMFNVFERVFEILRRRDIPAFVDLALET
                     GMLSPDADAEVYANLAEVMYARYGSFKIENFYKINPVRFGRMIKMRDLNAVGMRLRDI
                     ISEVRLPRRYIYFSRAVALILSIAIRLDDRVNVFMTAMPHLERYLRARQRSPLWYMRS
                     KNWQSALKNALTDKRLEVFRPLHHPREPEKKENRTAREAMLASLCALSGFAALQLQQT
                     GHPETAVLVLPASVIFGLIFLYKVMKD"
     gene            complement(5089..5487)
                     /locus_tag="PLG78_06345"
     CDS             complement(5089..5487)
                     /locus_tag="PLG78_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22765.1"
                     /translation="MKRYSLLILPLFMFCRTYVDIQPPAKGSVENPVLCDQPEGQRRY
                     LRLMRGPDGSPVKFEYLDAVQGPGGHILDRFKVENPARKTDKRSMFSQLWDYIASDPE
                     VPVYFRIYMDLYNPGKLDTEAVPGYKVGAE"
     gene            complement(5484..6332)
                     /locus_tag="PLG78_06350"
     CDS             complement(5484..6332)
                     /locus_tag="PLG78_06350"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNE22766.1"
                     /translation="MRRSILVFALLLVCAGCTKTVILQGYKRADTLLYVRTNNYFDLD
                     GAQSSFVKGKLKEHLSWHRSEELPRVADLLVEIARRGKEKFGHDDALWMESQILGMRD
                     RTLTRIEPDCSEFLTTLNEEQMVNFRAQFAKETSEIEEEFHANQDKKAALRAKRLTDF
                     MEFWTGSLDSAQKAKAQEIARDLPDLTAMRLAYRAHGMAQVERLVREKAGKEKTRDFL
                     RSFWLLPGARPDPVAKQYSNESWKSFRTGTERMDGILTKEQRKKAADKMIELAQDLRK
                     ISLESN"
     gene            complement(6346..7743)
                     /gene="fumC"
                     /locus_tag="PLG78_06355"
     CDS             complement(6346..7743)
                     /gene="fumC"
                     /locus_tag="PLG78_06355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNE22767.1"
                     /translation="MTRKERDTMGEIEVASDRYWGAQTQRSLHHFKIGHDHFPREFIR
                     AMGILKKAAAIANRELGLLSQEKVNLIMRAADEVIDGKLDDHFPLVIWQTGSGTQTNM
                     NANEVISNRAIELAGGKMGSKDPIHPNDDVNKAQSSNDTFPTGMHIAAAERIAQHLIP
                     AAKSLRDVLDKKSKEFEKIIKIGRTHLMDATPLTLGQEFSGYVSMLSNDIKRLEKVLP
                     GLYELALGGTAVGTGLNTHPKFADLAAKKIAELTKLPFVSAPNKFEALAGHGPLVETH
                     GVLKTLACDLMKIANDVRWLASGPRSGLGELSIPANEPGSSIMPGKVNPTQSEAMTMV
                     AAQVIGNDVAVNFGAASGNFELNVYKPLIIFNTLNSIRLLGDAMNSFREHCAEGIEPV
                     KENIQKHLTNSLMLVTALNPHVGYDAAAKIAKNAFNKNITLKESAQELGLVSPENFDK
                     WVRPEDMIAPDENVG"
     gene            complement(7755..>8505)
                     /locus_tag="PLG78_06360"
     CDS             complement(7755..>8505)
                     /locus_tag="PLG78_06360"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22768.1"
                     /translation="RDITLAGKIQKDLLPPNKSEGGPYQWHGFMRTMSGAGAGGDYID
                     ALEIRGHTWFAMGDVTGHGIQAGLLVMLARTSLHQVLRDQQVQDPGKVLSLLDYSFSR
                     TASSLTEKSFMTFVLMRFDANGKVTFAGSHLKILVYRPAEDRMDILETKGPWIGVLTA
                     DRRVSFDEFTFQLGIGDIVLLYTDGITEARDALGEAIEVKGLLNMFREALQESQDIEK
                     INHRIIDLLVHATGKNIFDDDLSLLTIRRAH"
BASE COUNT         1947 a         2079 c         2149 g         2330 t
ORIGIN      
        1 cgcacctgta ttgtaatgtt tcagtttcat catttccgca acgcggtcga gaatcgtgtg
       61 tcgcagtttt ccaattctga aatcaaagtc cgcgtcagcg gggggcgtga ttccatattc
      121 tttctctgag ttttcaagta gcacgcgtcc aaccatcagg aactggcgaa gcaaattgcg
      181 atgccccgct tctagaccca gtttcttttc gatcgtgcca atggactgcg ttagatgttc
      241 tatgatttgt tcgcgcgatg atttgatcac atacttaatg aatcccggta gcacggaaat
      301 gttcgcagtc ggttccgttt tgcgcgcgtc ctgcaaggcc gcgaaaccca gtttcaagaa
      361 tccgggctgg aatggcatga ggctgtcatt ttccccgcac atgggttccc cttctggaaa
      421 gatggcaagc ttgccgccag gcgcggagag aatttgtcgc gtcattcgca tggagtcgcg
      481 atctgcaacc cctggaagga tggagtatgc ccccacgctc tggaaaagtt ttccaacagc
      541 accaaacagg aaatcgaacg ctcttcttgt ggccatgtag tgaaaacgac tccccatcac
      601 attggcaatg tgataggcga tcatgggctc cgcctggctt ggatgattag taaagaaaag
      661 taaacgattg tgtctcaggc ttcgcagcat ttctctgtcc gccggtgtga tttccagttc
      721 gtggatattc tgatacgtgc gcaggctggc aggaagtgtt gcatccgcaa accatgctgc
      781 agcaagattg aaatctggcg ggatgaagaa tcgcttcatg attgcttgtg cgatctgccc
      841 gccgtggtgc aagcagattc tatgactggc tgacctgagt gattaccttt tcagccattt
      901 cgcggacccc ggcagacgga tcttttagca ggctctggag tggttcgagg attgcgcgga
      961 attgcagtcc ctctgaagcc agatctttga ttctatatag aacggaaagg cggatcgttg
     1021 tagtcctgtg cgaaagtagt ttcatgatgc tctcatagtc tgacaatccg gcgagagagc
     1081 gaatcaggac ctgaattaac gactctgtgt atgagtccgt actgaccgct ctgtctataa
     1141 acgattggat ggctggcatg gcagtggtaa gatcgcattt tgaattggaa agaaactcga
     1201 acagatggga agcggcgggg aggagtcgtg agtcgttcaa acactctgca atcgcatcgg
     1261 tagcgttgcg tccgatcatc gaaggattct gccgcgcagc gacagtcaac aaccgcagaa
     1321 gatcccctct ggaatcttgt agatctgaaa taaactggcc tgtgtatctt tcgaggcttg
     1381 cgcctgcctc tgcgcacgct tcgatcagtt ccacgagtcc gtagcgctca ctgtctttta
     1441 agagggagac tgcctcgcca agtatttggg ctgcattttg ttgtgtgatt tcgcttctga
     1501 ggaaacgcat gccgtcctgt ttcattctgg cgttcttgct tctgagagct ttcatcaagt
     1561 tgggcacaag aaaaggaatc ttctgtccgt tttcttttgc tctctccacg acgtagtttg
     1621 cgaaggcgcg aacgcgatcg tcatcgtgaa gagtgagcgc ggcgatgcga tctagatgag
     1681 gacagggtag atccctggat gcatgcgcga ataatgcctg gagacatccc tggagaacgc
     1741 aagagtcagt ggagtacagg atttccggaa gttcgtttga ttccccttga agagacatgc
     1801 atgaaagaac gaaggcagag ttcatcctgg tttcgcagtg ttctgcctgc atcgctcgcc
     1861 taatagtggt gagcgtgtgg cctgcgtcta cgccagatct cactgcggac gcaacggcca
     1921 gtgtgatctc tttcaagcag gtctggtttt gcagcgacag tgtatcgatt tcggtccatt
     1981 gctccgtgga taaaagatat tctgcgagtt tccaggccgc ctctgatcga atccattcat
     2041 ctacatgaga tgtttcagag cgcaggcttt gcaggagccg cggaagttgc tctttgtatt
     2101 ggatcgcgtc ttctccctgg agcattttca gtgctgcagc aggatgcagt ctttccagtt
     2161 caaatgattc gctcgtatgg ttgtcttcca cttctgaaga atatgaaaac tgatagatgt
     2221 tttcgcattc agtacaaaaa cgaaggtttc ctgattcgcg catttttttg atctgggcgg
     2281 gagtcctcaa ttcgtgattc caggactcga ctcggttcag gctgctgcaa atcgagcagc
     2341 gcctcacagt tggttctctc agtgctttta gaatcaaggc cgcgttctct gattgcggag
     2401 acgcgagtga tttttcgatc acagatttca gactcttgct ttcacttgcg aaatgaagca
     2461 ggaacggaat gagatcttct tgttttttgg cggcttgagc cagcttcgcg attgcatcta
     2521 ggctcggctc aaatttgttt cccgattgaa tcatcgaatc gatttgccgc agtgcaacgc
     2581 ttcgcacata cgaatcctgg tcgagcagat agagagtcaa tttgtcaatc cactccgcgc
     2641 gtctggtccc tctggatttt gggatagtat gcgccaggaa gatagcagtt cccgctcgca
     2701 caaaggaaga ctcattctca agtaaggagg agaatttttc cgcatccgtg gtggagtagt
     2761 gatccgccaa aaagtatgcg gcgcgctccc gtacatctga ctctgcattg ggatcatttg
     2821 caatgcgccc gagctcttcg acgatcgcaa tattcgcctt tgattcccct gtgaaccagg
     2881 gaagttcctg gatcgcgagt ttctgcgaat cctgataagt aagcaagcga gcgtagctag
     2941 aaacgtgttc cgttatgtcg attccggagg aggcggtagt agctaaaacg cggaccaggc
     3001 tcttgcgcga aaactcttcg gtcatcatca ggtcacagac ctgagccacg gttgccctct
     3061 tcttccttat ctgcattgcg atggcttcgg ctgctctgaa aggagaagct tcaaagtgaa
     3121 gaacgataga cggcattgca ggtccgatgt caaaagacct ggccagatcc gcgagtgctt
     3181 ctatcaccca ggttttgaaa tcgtcgtgaa ggaaagtgtt ttcgatttca tgggttcggt
     3241 tttctctgag aagttcaatc atcaccgctc gaatggcggg ctgcatcacc gggacttgat
     3301 gagtttcgta aaagattcgt agcagagggt cgagtgctgg aaaaatcgac tctccacttc
     3361 gagccaggcg ttcaaaatat tgggcggacg caatccagtt tggatataaa aagtcccgat
     3421 catcgtttgc gatggttcgc aggtgatgtt cgaattcgcc ggagttcatt tccctgcaaa
     3481 aatgttagtc tttcattact ttatagagga agatcaatcc aaatataacg gatgcaggca
     3541 gcaccaggac agccgtttct ggatgacctg tctgttgcaa ctgaagggcg gcaaatcctg
     3601 acagtgcaca tagactggcg agcattgctt ctctcgcggt gcgattttct tttttctctg
     3661 gttccctggg atgatgaagc ggtcgaaaga cttccagtct tttatctgtc agtgcattct
     3721 tgagagcgga ctgccagttc ttgcttcgca tgtaccagag aggactccgt tgcctggcgc
     3781 gaaggtatct ttccagatgt ggcatggcag tcatgaatac gttgactctg tcatccaggc
     3841 gaatcgcgat cgaaagaatg agcgccacag cccggctaaa gtagatgtaa cgtcgcggaa
     3901 gacgcacttc tgaaatgatg tctcgcaagc gcatgccgac cgcgttgagg tcgcgcatct
     3961 tgatcattcg accaaatcgc accggattga ttttatagaa gttctctatc ttaaaggatc
     4021 cgtatcgagc atacatcact tctgcaaggt tggcatacac ttccgcgtcc gcatccggag
     4081 acagcattcc agtttctagc gccagatcca caaaggcggg aatatcgcgt cttctcagta
     4141 tttcgaagac tctttcgaat acgttgaaca ttctttctgg aatcgattgt gtggcgccaa
     4201 agtctaccag gccaattcgc agaggacgta caccgtcagg cggaattaca aaaatatttc
     4261 ccgaatgcgg gtctgcgtgg tagaaacgat catcgaagat cattcttaca tacgtctcta
     4321 ctaaaagctc tgctgcttcg cgcctggcaa tgccgcgcgc atcgagctcc tctatttcag
     4381 aaatgtttat gccttcaatg tattctgtgg tcaagaccgt gccgccggac aattcatcca
     4441 caaccgatgg aactactact cgcggatcgt ttgcgaaatt gtctcgaatc cttcgcatgt
     4501 tctctgcttc gttttcgtag tctatttctg tcagagtcat ggtgctgaat tcatagagat
     4561 gagggcgaaa gtccagataa gggtaaaccc agaggatcag atttacggcg agtttgagag
     4621 cgcgaatgtc ctgacggatt tgagattcga tccctggatg tagaaacttt acggctacct
     4681 ttcttccgtc ttttgtccgg gccagatgca cctgacccag agatgccgcg gcaatcgccg
     4741 tgtgatcgaa ttcctgaaaa agggattcag gctcagatcc gaattcattt agaaaacgat
     4801 atcgtacctg cgcgtacgga cgcggagtga tcttgtcttg aacgctcgca aagacttccg
     4861 taaattcaga atcaaataga ttacttacag tagaaagaaa ctgtgcggcc ttgatgtaga
     4921 tgccacccat ttctatgaag aaagttgcga tcttctttgc agcccagaga gtgactttgt
     4981 ttcgcgcagc tgatctgcgt ggagcagaaa gcggttttgt tccgtgatag acaaaaacag
     5041 aaatgatcaa aagtcgaact gtaaaaatga acaaagagag agttcgcatc attcagctcc
     5101 gactttgtat ccggggacag cttccgtgtc tagcttgccg ggattgtata gatccatata
     5161 gattctgaaa taaaccggca cttccggatc gcttgcaata taatcccaca gttgtgagaa
     5221 catgctgcgc ttatctgtct ttcgcgccgg gttttctact ttgaatcgat ccagaatatg
     5281 gccgccagga ccctggactg catccaggta ttcaaacttt acggggcttc cgtccggtcc
     5341 tctcatcagg cgcaaataac gcctctgtcc ttccggctgg tcgcagagaa ccgggttttc
     5401 cacgctccct ttggctggag gttgaatatc cacgtacgtt ctgcagaaca tgaaaagtgg
     5461 caggatcaat agcgaataac gtttcaattt gattccagtg agatcttgcg aagatcctgg
     5521 gcaagctcaa tcattttgtc cgctgctttc tttctttgtt cttttgtcag aatcccgtcc
     5581 atcctttcag tgccggttct aaagcttttc catgactcgt tcgaatactg ttttgcgacc
     5641 ggatcgggtc tggcgcccgg aagcaaccag aagctgcgga gaaaatccct ggttttttct
     5701 ttgcctgctt tttcgcgcac gagtctttcc acctgtgcca ttccatgcgc cctgtatgca
     5761 agcctcatcg cagtgaggtc tgggaggtct cgcgcgattt cctgtgcctt tgctttttgc
     5821 gcagagtcca ggcttccagt ccagaattcc atgaaatcag tcagcctttt tgcgcgaagg
     5881 gcagctttct tatcctgatt tgcatggaac tcttcttcaa tttcggaagt ttcttttgcg
     5941 aattgcgcgc gaaagttcac catctgctct tcgttgagag tggtcaaaaa ctccgagcaa
     6001 tccggctcga ttcgagtgag cgttctgtcc cgcattccca gtatttgaga ttccatccaa
     6061 agcgcatcgt cgtgaccaaa tttctctttt ccgcgccggg cgatttcaac cagaagatct
     6121 gccaccctcg gaagctcttc agatctgtgc catgatagat gctctttgag ctttcctttc
     6181 acgaaggacg actgcgcgcc gtccagatcg aaatagttgt tcgtgcggac atagagaaga
     6241 gtgtccgccc ttttgtagcc ctgaaggatt actgttttcg tgcatccggc gcatacgagc
     6301 agtaaggcga aaaccagtat actgcgacgc atcacgacga atgcctcagc ctacgttttc
     6361 gtccggggcg atcatgtctt ccggacgaac ccacttgtca aaattctccg gagaaaccag
     6421 acccagctcc tgcgcagact ctttcaaagt gatgttcttg ttaaaagcgt tctttgcgat
     6481 tttcgcggca gcgtcatagc caacgtgagg attgagagcg gttacaagca tcagcgaatt
     6541 tgtgagatgc ttttgaatgt tctctttcac aggctcgatt ccttctgcac aatgctcgcg
     6601 gaagctgttc attgcatcac cgagaaggcg gatggagttg agtgtattga aaataatcag
     6661 aggtttgtaa acgttgagct caaaatttcc ggaagcggcg ccaaagttta cagcaacgtc
     6721 attgccgatc acctgggctg ccaccatggt cattgcttct gattgagtcg gatttacttt
     6781 tcctggcatg atagaagagc ctggttcatt tgcaggaata gaaagttctc caagaccgga
     6841 cctgggaccg ctcgcaagcc agcgcacatc gtttgcaatc ttcatcaaat cgcaggcgag
     6901 agttttgagt actccgtgcg tttctacgag tggaccatgg cctgccagtg cttcgaattt
     6961 gtttggagcg gaaacaaaag gcaactttgt gagttctgca attttctttg ctgccaggtc
     7021 tgcgaatttt ggatgagtat tgagtcccgt gcccactgcg gttccaccca gagccagttc
     7081 atacagtcca ggcagaactt tttccagacg cttgatatcg ttggaaagca tagaaacgta
     7141 gcctgaaaat tcctggccca gggtgagcgg cgttgcatcc atcaaatgag tgcggccgat
     7201 cttgatgatc ttttcaaatt cctttgactt tttatccagc acgtcgcgaa gactttttgc
     7261 tgccgggatg agatgctgtg cgattcgctc cgccgccgca atgtgcattc ctgttgggaa
     7321 agtatcgttg gaactttgag ctttgtttac atcgtcgttg gggtggattg gatctttgga
     7381 acccattttc ccgcctgcaa gttcgatcgc gcgattggag atcacttcat tcgcgttcat
     7441 gttggtctgt gtgccagaac cagtttgcca gattacaaga gggaagtgat catcgagctt
     7501 tccatcgatg acttcatctg cggcgcgcat gatgagattt actttctcct gcgagagcag
     7561 tccgagttcg cgatttgcaa tggccgcggc ctttttcagg atacccatgg cgcggatgaa
     7621 ctcgcgcgga aaatgatcgt gtccaatttt gaagtgatgc agtgatcttt gagtctgggc
     7681 tccccagtag cggtccgacg ctacttcgat ttcgcccatt gtgtcgcgtt ctttgcgtgt
     7741 catagggttg aaattcaatg ggcgcgtcgg atcgtcaata aagacagatc gtcatcgaat
     7801 atgtttttcc cggtcgcatg aaccaggaga tcaatgattc tgtggttgat tttttcaata
     7861 tcctgcgatt cctgcagcgc ttctcggaac atgttgagca aacctttgac ttcgatggct
     7921 tctcccagcg cgtccctggc ttctgtaatt ccgtctgtat aaagcagaac aatgtcgcct
     7981 atccctagct ggaatgtgaa ttcatcaaat gaaacgcgtc tatccgctgt aaggactccg
     8041 atccagggtc cctttgtttc cagaatatcc attctatctt ccgctggacg atacaccaga
     8101 atcttcagat gagaaccagc aaatgttact ttgccgtttg catcaaatcg catgagcaca
     8161 aacgtcatga aacttttttc agtcaggctg cttgcagtgc gtgagaatga atagtccaga
     8221 agagaaagga cttttccagg atcctgcact tgctggtctc gcagcacctg atgaagagac
     8281 gttcgcgcaa gcatcaccag aagccctgcc tgaatgccgt gtccggttac gtctcccatc
     8341 gcaaaccagg tgtgcccccg gatttcaagt gcatctatgt agtctccgcc cgcacctgct
     8401 ccagacatgg tacgcataaa tccatgccac tggtatggac caccttcgct cttgttaggc
     8461 ggaagaagat ccttctggat ctttcctgcc agagtgatgt ctctt
//
LOCUS       DAOQJL010000103         8501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4483_length_8501_cov_4.065475, whole genome shotgun sequence.
ACCESSION   DAOQJL010000103 DAOQJL010000000
VERSION     DAOQJL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_8501_cov_4.065475"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLG78_06365"
     CDS             complement(<1..966)
                     /locus_tag="PLG78_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNE22769.1"
                     /translation="MKRILDRYVFFEFLKSFVFSLLGLILMLLIARALVAFKVDTRQP
                     KIHMYLYLLYSIPDMVAFVLPMAMMFAVCFVVAQFTVSRELVAMLSAGVAFYRIVMPI
                     WIFAAILVPVLFVFQNFVATPANRMSSEEEAAFKKDSATIKDIVWQRNLRGREGFYFI
                     YYLDRKEQTIKGGFNYIELKHDKPVRMMQANSGKFDPATGEWTFSRVRNISFGDDLAV
                     LKVENEDTRVEKFPEDYDFFENPMRDASELNLFELAEEIARKKKMGFDSAPYSVQFHS
                     LLAFPFLCMIVAVIASIAGAGGNHRSTGPLIRALLVSAVTILIYYL"
     gene            complement(963..1805)
                     /locus_tag="PLG78_06370"
     CDS             complement(963..1805)
                     /locus_tag="PLG78_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22770.1"
                     /translation="MGIVLDLKDPGWKMTPMQVIRILPRNEAFDFNFKRKAWHMLGLL
                     VPAFFYFDPLVILGTPYIHITRMVGLGLNVFMLLALIVIDVLRFRVESLNQLFIRVAG
                     PLLKEQEHGRMNATIPYLTATLLLFSSTQEIIAGCATTFLMLGDPVAAYIGGKFGRVR
                     FWNSKSLEGMLAFFLAGTTAAGLFLLLHSWLILQGFPNAVREAEYSFFGNRPLALLAV
                     LVVGSAAAAVTEFFSSNRLAGLLDDNLEVPIMAAIAMAVTANLCGFSGESIFFDLKQI
                     FARG"
     gene            1846..2241
                     /locus_tag="PLG78_06375"
     CDS             1846..2241
                     /locus_tag="PLG78_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22771.1"
                     /translation="MIKNAAPFEGVKLRAVHLLRHLERVERDLEELKRLEQGLKDDRA
                     YTARLRDSLVDEQLRLEQSRARLLQQVVKAPPEKVVDAVQNQLDAIAAPQRRAPELIL
                     PEGKRKTEEKKPKRAVEEKKKPSFNFKYD"
     gene            2289..3674
                     /locus_tag="PLG78_06380"
     CDS             2289..3674
                     /locus_tag="PLG78_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22772.1"
                     /translation="MRFIRLIVLSVVAISAISCRLDSQVLAEFNGGDVRRSELRAAFR
                     LMEGQPSLEMQDRVVTDLALLKITALEAVKQGLDQTEDFKKREPLFHKLAFLGALEQE
                     LMAEQKKQKFKMMELQFAFLKEGEKDKKRKPEAEDIAKKLNEASDSDREKIIAQKTEM
                     KRYAVTGGYADPICVSCVPNPFAFLTDAAAKAEGKFVVVDDPNGFWIIRKAQETEVSG
                     DSLSETYEAYFKKAARLSRTAIAAMPEGAEKKEMEAQTMKEAQIETVSKQIAEQQVKR
                     ESQGLLQKRIEQRKKSLGLEALPGLRSYFDEKTDVAKDLSAPAIKMGGKDITLGELLQ
                     GLPEDKTVDRRQLLQQIVLPYEILKEDPLAAAALKSPQHDFLMAWNRNNQLAVLFLGK
                     QNLPEITEAQVLERFNAGFAGQNFGQLHDRIKADLTQEMRQGKLKQIQDGLGKQYNLK
                     INKDKLKGGEL"
     gene            complement(3717..4487)
                     /locus_tag="PLG78_06385"
     CDS             complement(3717..4487)
                     /locus_tag="PLG78_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HNE22773.1"
                     /translation="MSTMEHSEHISHAGHSHDGHGGHGASFGMRVGITMALLGVLLAF
                     SAAKVGGERAELVKCLVEQQNAHAKYQGNDVKHRVAAIALFQVHAEVAGASKGKSLNK
                     EDVGFLAQNVERYLQESAAAKTWVESYDPLIEVHVEAQEYYEFGQLSAEVAIVVASVA
                     LLLRRKEAWFVAVGLGILSIGIVIATFVHSSHIAHASEQKIEENFKAYREMRNAHKTT
                     QVEEKVVDEMLAWSGRPHLAKLESERHPESPGAKPDHK"
     gene            complement(4582..5886)
                     /locus_tag="PLG78_06390"
     CDS             complement(4582..5886)
                     /locus_tag="PLG78_06390"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNE22774.1"
                     /translation="MCDTAVALPNATREGITILAKNSDREPNEAQAIVRFDAAQHSEK
                     TLRVSYIEIPQVKETFEVILSKPFWMWGAEMGVNSKGVAIGNEAVFARVPFAKKNDGL
                     LGMDLLRLALERSDTAQNAMLCITQLLEQYGQDAMAGYYDKNFYYHNSFIIADPTEAY
                     VLETIEKHWAFVKVKDVRSISNGLTIETEFDGSSKGLIEFAKSKGLHKGGEFNFRTTF
                     SDKFFTYFSMCASRRADTEGNLRQSEGKTDARSAMSHLRSHGLHEKQGFRPDKSSMAS
                     VCLHAKGPVTPSQTTGSMVAELRKDGRHTVWLTGSSSPCLSLFKPFYFGTDTSPQSLC
                     GIPGSSADDSLFWKHERLHRRALKAYGPSHEMIRQAYDSLEEEFLRSEHDRFGQGSFS
                     SECVQRQLSAMQALDLPRPGSAVFHPLYRMQWNKWNSLCGIV"
     gene            5964..6767
                     /locus_tag="PLG78_06395"
     CDS             5964..6767
                     /locus_tag="PLG78_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE22775.1"
                     /translation="MSLRILARSPEYLVISKPAGLAFHQEGEEPGVMQILRQMETEGK
                     LPAGERLFPVHRLDTITSGILVIARGRKSANQIGNEFRFGRVEKVYCAISEKAPTRVM
                     GTISGDMEKAREGNWQLTRSQKDPAVTHFISVPYKTDRAMRLFLLRPATGKTHQIRVA
                     LKSIGSPILGDGRYGGYGRAREEERGYLHAYAIRFRSNGKTVQVIDYPTEGREFRREG
                     FLMALKQAGDPFSIRWPGLRSAGKRSFDPYEKPRKAKKKRPPKRRGSEA"
     gene            6806..7531
                     /locus_tag="PLG78_06400"
     CDS             6806..7531
                     /locus_tag="PLG78_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNE22776.1"
                     /translation="MKRTALLLLFTGMITAAELPSPYDADGRIAIIERFLSERRGLRL
                     FVIDNFEGERTYHTRAASTQLADIQFIKRSPDSAPYRRELDLIASRAQPFEHEKSLFL
                     HFSFEDPGKQVFQVLPDHPVQIPGIPQRISLWVNGQSTHALVLLFQNSDGREVRVNAG
                     RLDHAGWTRVEMTAPDLLRSRGRRLERRYTTQFLGFVIESSPREEAGDASVMIDNLLV
                     LTDLSELMIQGEEIPDTWGRTKR"
     gene            7563..>8501
                     /locus_tag="PLG78_06405"
     CDS             7563..>8501
                     /locus_tag="PLG78_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNE22777.1"
                     /translation="MPSADLSFSFSNDSLDQIVCDVIIAGVFQNGETWESSGLMEELD
                     RLASCATVMKEEKFDASRDKTILIRTAQTTLRSKRLIIAGLGKKEKITTRNLSRALRP
                     AIDQALGFDAVGTIAICLSEMPPVVDSILSAVDMSHDVCYASRESKKPPKSCDQIILM
                     SRYGLSGTEGHLKAAVTIARARSSAKDLTNMPPNIKRTTTLVEKAKSLSSIPNVTVDI
                     VEDRAWIEKEMPCFFTVARGSLATDPPKFIRAHYTPGGAKKKIAIVGKSVIFDTGGYQ
                     VKTDDYMNTMKADMTGGAMALSLIEGFAGLGLDRIAL"
BASE COUNT         2341 a         2203 c         2079 g         1878 t
ORIGIN      
        1 aagataataa attagaattg taacggcaga gaccagaagt gcacgaatca aaggcccggt
       61 agacctgtga tttcccccgg cacctgcaat cgaagcgatg acagcaacaa tcatacacag
      121 aaacggaaac gcaagaagcg aatgaaactg aaccgagtac ggggcagaat caaatcccat
      181 cttcttcttt cgtgcaattt cctctgcaag ctcaaacaaa ttcagctcgg atgcatcgcg
      241 catcgggttt tcaaagaagt cgtagtcttc cggaaatttc tcaactctgg tgtcctcatt
      301 ctctaccttg agaactgcca ggtcatcccc gaaagatata ttgcgaacac gcgaaaaagt
      361 ccattcgcca gtcgcgggat caaatttgcc tgaattcgcc tgcatcattc taacaggttt
      421 gtcgtgcttg agttctatgt agttgaaccc gcctttgatg gtctgttctt tgcgatccag
      481 atagtagatg aaataaaagc cttcgcggcc gcgcagattc ctctgccata caatgtcttt
      541 gatcgtagca ctgtctttct taaaggcagc ctcttcctcc gatgacattc gattcgccgg
      601 cgtggcgacg aaattctgaa atacaaacag aacgggcaca aggatggccg caaaaatcca
      661 gatgggcatt acgattctgt aaaaggcaac acccgctgaa agcatcgcaa cgagttcgcg
      721 cgagacagtg aactgagcca cgacaaagca aaccgcaaac atcatggcca tggggagaac
      781 aaatgcaacc atgtcaggaa tagaatacaa tagatacaga tacatgtgaa tcttcggctg
      841 acgtgtgtcc accttgaagg caacgagtgc ccgcgcgatg agaagcatca aaatcaatcc
      901 gagaagcgag aaaacaaaag acttcaggaa ttcaaaaaat acgtagcgat ccagtatccg
      961 tttcatccgc gcgcgaagat ctgtttgaga tcaaagaaaa ttgattcacc ggaaaaaccg
     1021 cacaggttcg cagtgaccgc cattgcaatg gcagccatga tgggcacttc caggttgtcg
     1081 tctagaagtc cggccaatcg atttgaagaa aaaaattcag tcacagcggc cgccgcggat
     1141 cctactacga gcactgccag aagtgctaaa gggcgatttc caaaaaaaga atactctgct
     1201 tcgcgtacag cattcgggaa tccctgaaga atcaaccagg aatgcaggag aagaaacaaa
     1261 ccagcggccg tggttccagc cagaaagaaa gcgagcattc cttctagcga tttagaattc
     1321 caaaaacgaa ctcgtccaaa ttttccacca atataagcgg ccactggatc tcccagcatg
     1381 agaaacgtgg tggcacaacc agctatgatt tcctgtgtgc tcgaaaacaa aagtagagtc
     1441 gcagtcaagt atggaatggt tgcgttcatt cgcccatgtt cttgttcttt gaggagaggt
     1501 cctgccacgc gaatgaacag ctgattgaga gattcaacgc gaaagcgaag aacatctatg
     1561 acgatcaacg ccagaagcat aaagacgttc agacccaatc caaccatgcg agtgatgtga
     1621 atgtaaggcg ttccgaggat cacaagtgga tcaaagtaga agaacgcggg cacgaggagc
     1681 ccgagcatat gccaggcctt gcgtttgaaa ttgaagtcaa aagcctcatt tcgcggcaag
     1741 attcgaatca cctgcatggg ggtcatcttc cacccggggt cctttaaatc gagtacaatt
     1801 cccatttcag actgccctgg cgcctgccga aaattgaact tgacaatgat aaaaaacgca
     1861 gctccttttg aaggcgtgaa gcttcgcgcg gtgcatctct tgcgtcacct cgaacgggtg
     1921 gagagagacc tggaagaact caagagattg gaacagggac tcaaggatga tcgcgcatat
     1981 acagcgcggc tgcgcgactc tctagtcgac gagcaacttc gcctcgagca atcccgcgct
     2041 cgcctcctcc agcaagtagt caaggcaccg ccggagaaag tcgtcgatgc ggtgcaaaat
     2101 cagctcgatg ccatcgcggc gccacagcgg cgcgcccctg aattgattct accggaaggc
     2161 aaacgcaaga cggaagagaa aaaaccaaag cgcgcggtgg aagaaaagaa gaagccatcc
     2221 ttcaacttca agtacgattg aatcgccgat tgtacttgcc cgcacacctg tcccggcaaa
     2281 atcgaacaat gcgtttcatt cgactcattg ttttgtctgt tgtggcgatt tcagccatat
     2341 cctgtcgcct ggattcccag gttctcgcag agttcaacgg cggagatgtt cgccgatccg
     2401 aactgcgtgc cgcctttcgc ctcatggaag gccagcccag cctcgaaatg caggacagag
     2461 tggtgacaga tctggctctt ctcaaaatca cagccctcga agcagtcaag cagggtctcg
     2521 atcagacaga agatttcaaa aaacgcgaac ctctgtttca caaactcgcc ttcctcggtg
     2581 cgctggaaca agaactcatg gcagaacaga agaaacagaa gttcaagatg atggaacttc
     2641 agtttgcctt tctaaaggaa ggcgaaaaag acaagaaacg caaaccggaa gcggaagaca
     2701 tcgccaaaaa attgaacgag gcatccgatt ccgaccgcga aaagatcatc gcacagaaaa
     2761 ctgaaatgaa acgttatgcg gtcacaggcg gctatgctga cccgatctgt gtatcgtgcg
     2821 ttcccaatcc atttgcattt ctaacagacg ccgctgcaaa ggcagagggc aagttcgtag
     2881 tcgtagacga tccaaacggt ttctggatca tccgcaaagc tcaggaaaca gaagtgtcag
     2941 gcgattctct ttctgaaaca tacgaagcat acttcaaaaa ggccgccaga ctttcgcgca
     3001 ctgcgattgc ggccatgccg gaaggggctg agaaaaaaga aatggaagcc cagaccatga
     3061 aggaagcgca gatcgaaact gtgtccaaac aaattgcaga acaacaggta aagagggaat
     3121 cccagggctt gctgcaaaag agaattgagc agagaaagaa atcgctcgga ctcgaagctc
     3181 ttcctggcct ccgttcctat tttgatgaaa aaacagatgt tgccaaagat ctgagcgcac
     3241 ccgccatcaa aatgggaggc aaagacatta cactcggtga acttcttcag ggtctgccgg
     3301 aagacaagac cgtagaccgc agacaactac tgcagcagat cgttcttcct tatgaaatcc
     3361 taaaagaaga tccacttgcc gccgctgctc tcaaatcgcc tcagcatgat ttcttgatgg
     3421 catggaacag aaacaatcag ctcgctgtcc tcttcctcgg gaaacagaat ctgccagaaa
     3481 ttaccgaagc tcaggttcta gaacggttca atgctggatt tgcaggacag aatttcggac
     3541 aactgcacga tcgcatcaaa gcagatctaa ctcaggaaat gcgtcaggga aaactcaaac
     3601 agatccagga cggcctgggc aagcagtaca atctcaagat caacaaagac aaactcaagg
     3661 gcggcgaact ctaatccgcg acgctcctgg tggatcacac caggagccgt accacgctat
     3721 ttgtgatccg gtttcgcacc tggcgattct ggatgcctct ctgattcgag ctttgcaagg
     3781 tgcggtcttc cagaccacgc gagcatttcg tccactacct tctcttctac ctgcgttgtc
     3841 ttgtgtgcgt ttcgcatctc acggtaggct ttgaagttct cttctatctt ctgctcggaa
     3901 gcatgcgcaa tgtgagagga atgaacaaac gtagcgatta caataccgat gctgagaata
     3961 cccagaccta ctgccacaaa ccatgcttct ttgcgtctga gcaagagcgc gacagatgca
     4021 accacgatcg ccacttctgc agacagctgg ccgaactcat agtattcctg cgcctccacg
     4081 tgcacttcaa tgaggggatc gtaggattca acccaggtct ttgctgcagc agactcttgc
     4141 aaatatctct ccacgttctg cgcaagaaat ccaacgtcct ctttattgag agactttcct
     4201 ttgcttgctc ccgccacttc tgcgtgcacc tgaaaaagag caatagctgc cacgcggtgc
     4261 ttcacatcat ttccctgata cttcgcgtga gcattctgct gttcgacgag acacttcaca
     4321 agttctgcgc gctcacctcc cactttcgcc gcggaaaacg caagaagaac tccaagcagc
     4381 gccatcgtaa tgccaacgcg cattccaaaa gaggctccat gtccaccgtg accgtcgtgt
     4441 gaatgaccgg cgtgtgatat gtgttcgctg tgttccattg tactcatcta gatctccagg
     4501 aagcgagtgg ctccgtcgcg tgcaatgaat gagtcagaga cgatccggtc gaatcttttt
     4561 ttgagacaac gcctgacctg ctcagacaat cccgcaaaga gaattccatt tgttccattg
     4621 catgcgatag agcggatgaa agaccgcgga gccaggacga ggaaggtcga gagcctgcat
     4681 ggcagaaagc tgccgctgta cacattcgga agaaaaactc ccctgcccaa aacgatcgtg
     4741 ctctgagcgt aaaaattctt cttcgagaga atcataagcc tggcgaatca tttcatgaga
     4801 gggcccgtat gccttgagtg cccgtctgtg aagccgctcg tgtttccaga acaacgaatc
     4861 atctgcagaa gatcctggga tgccacacaa ggactgcgga gacgtgtctg tcccgaaata
     4921 gaacggcttg aacagcgaca aacatggcga agacgatccg gtgagccaca cagtgtgtcg
     4981 gccgtccttt ctcagctccg ccaccatgga acccgtggtc tggcttggtg ttaccgggcc
     5041 cttcgcgtga aggcatacgg aagccatgct cgatttgtca ggacgaaagc cctgcttctc
     5101 gtgcaggcca tgagatcgca gatggctcat ggcggatctg gcgtctgtct ttccctcgct
     5161 ctgccggaga tttccttctg tgtccgctct tctggacgcg cacatggaga aatatgtgaa
     5221 gaacttatcc gaaaaagtag ttctgaaatt gaactccccg cccttgtgta agcctttgct
     5281 tttcgcaaat tcaatcaggc ctttcgaact tccgtcaaat tctgtttcaa tggtgagtcc
     5341 gttagaaatg gaacgaacat ctttcacttt cacaaacgcc cagtgtttct caattgtttc
     5401 tagaacgtat gcttctgttg gatcggcgat gatgaacgaa ttgtgataat agaaattctt
     5461 atcatagtag ccggccatgg cgtcctggcc gtattgctcc agcagttggg tgatgcagag
     5521 cattgcattt tgtgctgtat cacttctttc aagcgcaagg cgaagcaggt ccatcccgag
     5581 aaggccgtca ttctttttgg caaaaggaac gcgagcaaac actgcctcat tcccgatcgc
     5641 cactcctttc gagttgactc ccatttccgc gccccacatc cagaagggtt ttgagaggat
     5701 gacttcaaaa gtttccttta cttgaggaat ctcaatatat gatacacgta aagttttctc
     5761 cgaatgctgt gctgcatcaa atcggacgat tgcctgggcc tcattgggtt ctctgtcaga
     5821 attcttggca agaatggtga ttccttctct ggtggcattg ggcagggcta cggctgtgtc
     5881 gcacatggac aagttatgaa gagtcggtca gatcagacaa gttttttgga tcgtattgga
     5941 aaccgcttcc gaaaggctgg ctcatgtccc tgcgaattct cgcacgatct ccggagtatc
     6001 tagtcatttc aaaaccggcc ggccttgcct ttcatcagga aggggaagaa ccgggagtca
     6061 tgcagatcct tcgccagatg gaaacggaag gaaaactccc cgccggtgaa agactgtttc
     6121 cggtccatcg cctggacaca atcacatcgg gaattctagt aatcgcacgc ggaagaaagt
     6181 cagcaaatca aatcggcaat gaattccgat tcggacgtgt agaaaaagtg tactgtgcga
     6241 tctctgaaaa agcccccacc agagttatgg ggaccatctc cggagacatg gagaaggcgc
     6301 gcgaaggaaa ctggcaattg actcgcagcc agaaagatcc ggccgtgacc cacttcatca
     6361 gtgtgcccta caaaacagac agagccatga gattgtttct tctccggcca gccaccggaa
     6421 agactcatca gatccgcgtt gcactcaaat ccattgggtc accaattctc ggagatgggc
     6481 gttatggtgg ttatggacgc gctcgcgaag aagagagagg ctaccttcat gcctacgcga
     6541 ttcgatttcg ctcaaacgga aaaacagtcc aggtgataga ctacccgaca gagggccgcg
     6601 aatttagacg agagggcttc cttatggcac tcaagcaagc gggagatcca ttctcgattc
     6661 ggtggcctgg cctcagaagc gcgggcaaac ggtcttttga tccgtacgaa aagccgcgca
     6721 aagcaaagaa aaagcgtcct cctaaacggc gtggctcaga agcctaggct catgtaaaac
     6781 gcggaatctt ccgtcactgt tatatatgaa gagaactgct cttcttttgc tctttacagg
     6841 aatgatcacg gcggctgagc ttccttcccc atacgatgcg gacggtcgga tcgctatcat
     6901 agaacgattt ctatccgaaa ggaggggact gcgtctcttc gtgatcgaca atttcgaagg
     6961 cgaacgcacg tatcatacca gagccgcatc cactcaactc gctgacattc aattcatcaa
     7021 aagatcgccg gactccgccc cttatcgccg tgagctggat ctcattgcct cccgcgctca
     7081 acctttcgaa catgagaagt ccctgtttct tcacttttca ttcgaagatc caggcaaaca
     7141 ggtgtttcag gtactgccgg atcatccggt gcagattcca gggatcccgc aaagaatatc
     7201 actatgggtg aacggacaaa gcacccatgc tctggtcttg ttgtttcaga attcagatgg
     7261 acgcgaagtg agagtgaacg ctggcagact ggatcacgcc ggatggacca gagtggagat
     7321 gacagcaccc gatctcttga gatctagagg tagaagactc gaacgcagat acaccactca
     7381 gttcctgggc tttgtgattg aatcgagtcc acgcgaagaa gcaggcgacg caagcgtcat
     7441 gatcgacaac cttcttgttc taacagactt gagcgagctt atgatccagg gagaagaaat
     7501 cccggacacc tggggtcgga caaaaaggta gacacattta cggcggaaag gacaatgatt
     7561 tcatgccttc cgctgacctc tccttctctt tttccaatga ttctctggat caaatcgttt
     7621 gcgacgtaat cattgcaggt gtttttcaaa acggagagac ctgggaatct tcgggcctca
     7681 tggaggaact cgacagactt gcttcctgcg cgactgtaat gaaggaagaa aagttcgatg
     7741 catccaggga caagaccatc ctgatccgca cagcgcaaac aactcttcgc tcaaaaagac
     7801 tgatcatcgc aggtctgggc aaaaaagaga aaatcacaac acgcaatcta tctcgcgcac
     7861 tcaggccggc cattgatcag gccctgggat ttgacgcagt gggaaccatc gccatttgtc
     7921 tcagtgagat gcctcctgta gtcgattcga ttctctccgc agtcgatatg tcgcatgacg
     7981 tctgttatgc gtcgcgcgaa tctaagaagc caccaaagtc gtgcgatcaa atcatactca
     8041 tgtctcgcta tggtctttct ggcacggaag gacatctcaa agcagcagtg accattgcgc
     8101 gagcacgcag ttcagcgaaa gatctcacga acatgccgcc caatataaag cgcaccacca
     8161 ctcttgtaga gaaagcaaaa tcactctcct ctattccaaa cgtcaccgta gacatagtag
     8221 aagatcgcgc ctggattgag aaagaaatgc cctgcttctt tacagtggcc agaggttctc
     8281 tggcaacgga tcctccgaag ttcattcgcg cccactacac ccccggaggc gcgaagaaaa
     8341 aaatcgccat cgtgggaaaa agcgtcatct tcgacacagg cggataccag gtcaaaacag
     8401 acgactacat gaatacgatg aaggcagaca tgacaggcgg cgccatggca ctttctctta
     8461 tagaaggatt tgcgggcctg gggctagacc gaatagcatt g
//
LOCUS       DAOQJL010000104         8493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4490_length_8493_cov_4.882318, whole genome shotgun sequence.
ACCESSION   DAOQJL010000104 DAOQJL010000000
VERSION     DAOQJL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4490_length_8493_cov_4.882318"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLG78_06410"
     CDS             complement(<1..415)
                     /locus_tag="PLG78_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22778.1"
                     /translation="MKGHNRLLREIRQENSASYHLTRMVASPGWSVLSVRKLWQNRIV
                     LFQLAVYDFRHEYLGTWFGFLWAFVHPLLSASILWFVFSHGFRASRTAGDHFFLWLLS
                     GLFAWQFASLSIRGGALSIVQNSFLIRKLAFHVEFL"
     gene            414..2678
                     /locus_tag="PLG78_06415"
     CDS             414..2678
                     /locus_tag="PLG78_06415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE22779.1"
                     /translation="MNLPQFLDFLKQDIQFGKNLTGAFTIAGQDASFAPIPSSLDSRL
                     VAGLGARGLDKLYTHQALAVSSALAGKNTLVVTPTASGKSLTYMLPIFQKKLSSPQSR
                     ALLLFPTKALSQDQQASINEFNTHCDTNFKIFTFDGDTSPEARRRIMEAGDFVVTNPD
                     MLHAGILPHHTLWIKLFENLDFIVIDELHIYRGIFGSHVANLLRRLLRICAFYGKHPQ
                     ILCASATIGNPKEHAERLTGLPFELIDQNGAPRGDRHVLFYNPPVVNRQLGIRASALK
                     ETAALGSYLLKNRIRVIFFCRSRLRVELLYTYLVERCPELKDKIRSYRGGYLPDERRR
                     IERELREGSVLAVVSTNALELGVDIGMLDVAVCMGYPGSVSSLLQQFGRAGRRNQESL
                     SILVATSDGTDQYLIQHPDQFFEKNPEQAMTNPDNLLIVADHIKCATFELPFKKEESF
                     GSYPATLEILEHLAENRVIHGSGESFHWMSDVYPANTISLRSGPRENFAIIDCTEKGR
                     EEVIGEVDYFSAPTMIHQHAIYIHQGVQYYVDDLLWEERQARVRRIEVDYYTDAHEKV
                     DIEVLEDQARRVHNWFELSRGEFSIRTKAVMFKKIKLETHENLGWGDIHTPETEMHTQ
                     GAWILIPEDHPVRAGLAESELGAMLAGVSYSIGVVSPLFVLCDPRDMRFRSEVRSTVF
                     HKPAIFFYDSFPGGMELSHRVLENLAGIALSAHELVSRCGCANGCPSCIGLPDDESPV
                     KANVLKFLTALCDA"
     gene            complement(2682..3227)
                     /locus_tag="PLG78_06420"
     CDS             complement(2682..3227)
                     /locus_tag="PLG78_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22780.1"
                     /translation="MSDAWDDLSDHELESLRKAEEKFKVIKQQSNPFEHLQALAQREI
                     FKVNFSSYSGKDAGDASPLVIHADRTRLLPLISVAHTMWLPEFEEVATLVLDAEAAAK
                     KGEPESDDLEWNLQSFGLRRTPESYIVGYGENSSPLVLNKKMLLEFLSFLASSLSILE
                     HREGRNGQTMADLEPRFLQFI"
     gene            complement(3229..>3501)
                     /locus_tag="PLG78_06425"
     CDS             complement(3229..>3501)
                     /locus_tag="PLG78_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22781.1"
                     /translation="ADRITIVSLDESNIFTFSASVTTWKKDALDYVVESVHIEVGPYS
                     KMSLPWRERSYVVFGLRQFGYPGTVLLDILTSPVQLLYLAAGPGAK"
     assembly_gap    3502..3601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3779..4744)
                     /locus_tag="PLG78_06430"
     CDS             complement(3779..4744)
                     /locus_tag="PLG78_06430"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE22782.1"
                     /translation="MFESVKKWLRGRPGPRRLMILNPTSAKGQALQNFDRIQVRLREM
                     LGTYDLKFTRGPGDATEMVRRALKEREYRQILVAGGDGTIHEAMNGYFEKGRSLSKDI
                     PLGVINLGTGGDFYKTLRAMSPDYAAALMRNQFRLVDCGEASIEPMGYTRYFMNIASA
                     GIAGQVLKNLKASRFQAGTAAFFYHTLSTLVKYTPARVKITFTDTDGRHHDFEERIIN
                     FFACNGKFNGGGMKWAPFGNLDDGAFEIVIIRRTSKLRMILDSRKVYTGHVEGMEGVS
                     QFETKEILLQPIDPITFELDGEVPPIASPKSIRFRNIHAQLPVVL"
     gene            complement(4737..6632)
                     /gene="mnmG"
                     /locus_tag="PLG78_06435"
     CDS             complement(4737..6632)
                     /gene="mnmG"
                     /locus_tag="PLG78_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNE22783.1"
                     /translation="MANPVPSRFDVVVVGGGHAGAEAAHACARSGLRTLLLTMNLDTI
                     GQMSCNPAIGGIAKGHMVREVDALGGIMGRVIDQTGIHFKMLNRSRGPAVWAPRAQAE
                     KKAYQNHIKWTLEATPNLSFRQDTCDSLIIENHKVCGIICSRGQEIQCDYVILTTGTF
                     LQGLIHIGEYQARSGRIAEQSAMKLSDCLTAYGFELGRLKTGTPPRVLKKSLDLSVME
                     EQEPDPVPSPFSYSTSAITQPQISCYVTYTGAPTHEIIRQNLDRSPMYSGQIKSTGPR
                     YCPSIEDKIVRFADKERHQVFIEPEGVETGEVYLNGVSTSLPEEVQWQVVRSIRGMEN
                     AEIIRPGYAVEYDFVDPRELHHSFETRKIQNLFFAGQINGTTGYEEAAAQGMIAGINV
                     LRKSRNESPFILGRGEAYIGVLVDDLVLKGVEDPYRMFTSRAEHRLLLRQDNADERLM
                     KYGFELGLLGEDSYRAMLDRGQRIKKMRDLISTRGIRATQEVADLFAKKNMEMPKSGF
                     GKTLDSLLRRPEVSIEDLTVIIPELTELTRDERSVLELEIKYAGYVKRELEKVDRRER
                     ISNHPIPANFDFDTVPGIKKEAREKLKRFRPHDLNTASRISGVDPPDIDLIYLKILEA
                     EKAQSHV"
     gene            6727..7698
                     /locus_tag="PLG78_06440"
     CDS             6727..7698
                     /locus_tag="PLG78_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE22784.1"
                     /translation="MRYTIKNLVFKGGGVLGVAYAGAVEVLSRAGILAGVENLAGTSA
                     GAIASLLLSLRYDEETLKKILLDLDFKQFTSKAEPLRFQDRYGWHSTQPVREWLEDQV
                     RKSRQPGELSGRETFQDFKDLGCRNMHVFATNLNTRSVEEFSVRRTPEVRVVDAVLAS
                     LSIPAYFQSFQFPDGKPNSHIYVDGGALLNYPITAFDTPDKNRRGALKANPDTIGFHL
                     ELLGHEFPTSDLEFGTFSKWAQTLYETVKDVQSTLLLSSPEHMERTVFIDNGGISPID
                     FEITPEQKTWLIESGRQATEAYLQLYRYRKSFRSRALRSLMRWRTRR"
     gene            7773..>8493
                     /locus_tag="PLG78_06445"
     CDS             7773..>8493
                     /locus_tag="PLG78_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22785.1"
                     /translation="MAAPEKPREKFRLLNSMRIALSLFFLALALRESHPNFLYFIVAH
                     LVFSLVWILFVEMDRLPRGRWIGYIPATLDIGAVTVALLLTGHARSPLVAGYFGLAAI
                     SSTLQNRTFGLYVACLSVISYAAASAGVTFGWLTSIDVLAEGPQTISPAGILVYTLLV
                     AIGLTIVHTVCFQFVNSNRRILGEANLLLEKVAMQEEQLRLRNAQMEQDLDLARSIHF
                     RLLPESLPEIPGLTFATLYVPQ"
BASE COUNT         1973 a         2074 c         2092 g         2254 t
ORIGIN      
        1 ggagaaactc tacatggaac gcgagctttc gaatcaagaa gctgttttga acaatggaaa
       61 gggccccacc ccgaattgac agggatgcaa actgccacgc aaacaatccg gatagtaacc
      121 acagaaagaa atgatcaccc gccgtccggg atgcgcgaaa tccgtgagag aatacgaacc
      181 acagaatgct tgcgcttaac aacgggtgaa caaaggccca gagaaaacca aaccatgtgc
      241 ccagatattc atgcctgaag tcatagaccg caagctgaaa cagtacgatt ctattctgcc
      301 atagtttgcg cacggagagc acgctccagc ctgggcttgc aaccatgcga gtcaagtgat
      361 acgaagcgga attctcttgc cggatctcgc gaaggaggcg attatgtccc ttcatgaatc
      421 tcccgcagtt cctggatttt ctgaaacaag acattcagtt cggaaagaac ctgaccggag
      481 cattcacgat cgcaggtcag gatgcttcct ttgcgcccat tccgtcgtcc ctggattcaa
      541 gacttgtggc cgggcttggt gcgcgcggcc tcgacaaact ttacactcac caggcgcttg
      601 cggtttcttc tgctcttgca ggcaagaata ctctggtcgt gactcccacc gcatccggca
      661 aatcgctcac ttatatgctt ccgatttttc agaagaagct ttcatcccct caaagccgcg
      721 cattgctgtt gtttccaaca aaggctctca gtcaggatca gcaagcgtcc atcaatgagt
      781 tcaataccca ttgcgataca aactttaaaa tctttacttt tgacggagat acttccccgg
      841 aagcgcgaag acgaatcatg gaggccggtg atttcgttgt aaccaatcct gacatgttgc
      901 acgcgggcat tctaccgcat cacacactgt ggatcaaact attcgagaat ctggatttca
      961 ttgtaattga tgagctccac atctaccgcg gaatttttgg aagtcatgtg gccaaccttc
     1021 ttcgccgatt gcttcgcatc tgcgcgtttt acgggaagca tccacagatt ctttgcgcgt
     1081 ctgccaccat tggaaatcca aaagaacatg ctgagcgcct aacgggtcta ccatttgaat
     1141 tgatcgatca gaacggagct cctcgtggag atcgtcatgt gttgttctac aatcctcctg
     1201 ttgtaaatcg gcagctggga atcagagcgt ctgctctcaa ggaaacagcg gcccttggat
     1261 cctacttgct caagaatcga attcgggtga tcttcttttg cagaagcagg ctcagagtgg
     1321 agctcctcta tacttatctt gtagaaaggt gtcctgaact aaaagacaag attcgatcct
     1381 acagaggtgg ctatcttccg gatgaacgcc gtcgtatcga aagagaattg cgcgaaggat
     1441 ccgttctcgc cgtggtcagc acgaatgctc tcgagctggg tgtggatatc ggcatgctgg
     1501 acgttgccgt ttgcatggga tatcctggtt ccgtcagttc attgcttcag cagtttggcc
     1561 gcgccggcag aaggaatcaa gagtctcttt ctattctggt ggccacttca gatggaacgg
     1621 atcagtactt gatccagcat ccagatcagt ttttcgagaa gaatcctgag caggcaatga
     1681 ccaaccctga caatctgctg attgttgcag atcacatcaa atgcgcaacg ttcgagcttc
     1741 ctttcaagaa ggaagaatca tttggatcgt atcccgcgac tctggaaatc ctggaacacc
     1801 tggcagaaaa tcgtgtgatt catggatccg gtgagtcgtt tcactggatg agcgacgtat
     1861 atcccgctaa tacgatcagc ctcaggtctg gcccgcgcga gaattttgcg atcatagatt
     1921 gtacggaaaa aggacgagaa gaagtcatag gcgaagtgga ttatttttca gcacctacta
     1981 tgattcatca acacgcgatt tacattcacc agggcgttca gtactacgta gatgatttat
     2041 tgtgggaaga acgtcaggcg cgcgtgcgca gaatcgaagt ggattactac acggacgcgc
     2101 acgaaaaggt ggatatagaa gtgctcgagg accaggccag gcgagttcac aactggtttg
     2161 aactttctcg aggcgaattt tccattcgca caaaggcggt gatgtttaag aagatcaagc
     2221 ttgagactca tgaaaatctt ggctggggag atatccacac accagaaact gaaatgcaca
     2281 ctcagggtgc ctggattctg attcccgagg atcatccagt gagagccggg ctggcggaga
     2341 gcgagctggg tgccatgctt gcgggagttt cctattctat tggagtggtc tcgcctttgt
     2401 ttgtgctttg cgatccacgc gatatgcgat ttcgttctga ggttcgttct actgtctttc
     2461 acaaaccggc gattttcttt tacgattcat ttccaggtgg aatggaattg agtcatcgtg
     2521 tgctcgaaaa cctggcaggc attgcactgt ctgcgcacga gctcgtgagt agatgcggat
     2581 gcgcgaacgg ctgtccatcg tgcattggtt tgccggacga cgaatctcct gtgaaggcca
     2641 atgtgctcaa gtttctcacg gcgctttgtg atgcgtgagt tctatataaa ttgaagaaag
     2701 cgcggttcca aatccgccat ggtctggccg tttcttcctt ctctatgttc aagaatggaa
     2761 agtgaactgg ccagaaagga aagaaactcg agaagcatct tcttgttcag tacaagcgga
     2821 gaactatttt ctccgtagcc tacgatgtac gattcgggag ttcttcgcaa tccaaagctt
     2881 tgcagattcc attccagatc gtctgattca ggttctcctt tcttcgccgc ggcctctgca
     2941 tccagaacga gtgtagccac ttcttcgaat tcaggcagcc acatagtgtg tgctactgaa
     3001 atcaagggca gcaatcgagt cctatccgca tgaatgacca gcggactggc gtctcctgcg
     3061 tctttgccgc tgtaggacga aaagttcact ttgaagattt ctctttgtgc cagtgcctgc
     3121 aaatgctcaa acggatttga ttgttgtttg atgactttga acttctcttc tgccttgcgc
     3181 agggattcca gttcatgatc gctcaaatca tcccacgcat cgctcatgtc atttggctcc
     3241 cgggcctgcc gccagataca gtagttgcac agggctcgtg aggatgtcca gaagaacggt
     3301 cccaggataa ccaaattgcc tcagtccaaa aacaacgtaa ctgcgctctc tccacggaag
     3361 agacattttg gaatatggac ccacttcgat gtgcactgac tctactacat aatccagggc
     3421 gtcttttttc catgtggtga cggatgcgga aaaggtgaag atattggatt cgtctaaact
     3481 aacaatggta atgcgatcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 ngtggagccg tcggtcctgg agacagcgtg gccagggatg gtctttttcc cgcttttgaa
     3661 ctcgtgacgg ggattcgcgt agtccaccgt ttcgtcatgg tagtgacttg tgatgcattg
     3721 tccggttgag actgccagta gaacaggcaa aaggtatttc tttcgattca tctttcctct
     3781 ataagacgac aggaagttgc gcatgtatgt ttcggaacct aatggattta ggagatgcga
     3841 tgggaggaac ttcgccatcg agttcaaaag tgatcgggtc gatgggctgg agtagaattt
     3901 cctttgtctc aaactgagaa acgccttcca tcccttccac gtgtcccgtg tagacttttc
     3961 tactgtccag aatcatgcga agcttgcttg tgcgacggat gattacgatt tcaaaagctc
     4021 catcatccag atttccaaac ggggcccatt tcattcctcc gccattgaat tttccattgc
     4081 acgcgaagaa gttgatgatg cgttcttcaa aatcgtgatg tcttccgtct gtgtctgtga
     4141 aagtgatttt tacccgggcc ggagtatact tgacgagagt gctcagtgtg tggtagaaaa
     4201 atgcagccgt tcctgcctga aatctggatg ctttcaaatt cttcaatacc tgacctgcga
     4261 ttccggcaga tgcgatattc atgaaatagc gagtgtagcc catgggttcg atcgacgctt
     4321 ctccgcaatc caccagacga aactgatttc gcatgagagc tgcggcgtaa tcagggctca
     4381 tcgcacggag agttttgtag aagtctcctc cggtgcccag gttgatcacg ccgagtggaa
     4441 tgtctttgct caagcttcgg cctttttcaa agtatccatt catggcctcg tggatggttc
     4501 cgtcaccgcc cgcgaccagg atttgtctgt attcgcgttc ttttaacgcg cgacgaacca
     4561 tctccgttgc atccccgggg cctcgagtga atttcagatc ataggtgccc agcatttcgc
     4621 gcaatctgac ctggattctg tcgaagttct ggagagcctg acctttcgcg ctggttggat
     4681 tgagaatcat caaccgacgt ggtccgggtc taccacgcag ccattttttg acagattcaa
     4741 acatgcgatt gggccttttc tgcttctaaa attttcaaat agatcaaatc gatatccggt
     4801 gggtccacac cggaaatacg cgaggcggta tttagatcgt gaggtctaaa tcgttttagt
     4861 ttttcgcgcg cttctttctt gattccaggg actgtatcaa aatcgaaatt cgcgggaatt
     4921 ggatggttgg atattctttc gcgtctgtct actttttcga gttcgcgttt cacatatcca
     4981 gcatacttga tttccaattc tagaacactt cgctcgtcgc gcgtaagttc agtgagctct
     5041 gggataatta ctgttagatc ttcaatgctg acttctggcc tgcgcaaaag tgaatcgagt
     5101 gttttaccaa atcctgactt tggcatttcc atgttctttt tggcaaacag atccgcgact
     5161 tcctgtgtgg cgcgaattcc ccgagtcgaa atcagatcgc gcatcttttt gattctttgt
     5221 ccacgatcga gcatagctct gtacgaatct tctcccagca gtcccagctc aaacccatat
     5281 ttcatgagtc gttcgtctgc attgtcctgt ctgagaagaa gtctgtgttc cgcgcggctg
     5341 gtgaacattc tgtagggatc ttctaccccc ttgagaacca gatcgtctac gagaacacca
     5401 atgtatgctt ctccgcgtcc caggatgaag ggagattcat ttcttgattt gcgaagcaca
     5461 ttgattccag cgatcattcc ctgcgcggct gcctcttcat atccggtggt tccgttgatc
     5521 tgcccggcaa aaaacaaatt ctgaattttg cgcgtctcga aagaatgatg caactctcgc
     5581 ggatcgacga aatcgtattc aacagcatat ccagggcgaa tgatttccgc gttttccatg
     5641 ccgcgaatgc ttcgcacgac ttgccattgt acttcttccg gtagacttgt acttacgcca
     5701 ttaagataaa cttctcctgt ctccacacct tctggttcaa tgaatacctg gtggcgctct
     5761 ttgtctgcga atcgtacaat tttgtcttca atcgaagggc aataacgtgg ccctgtactt
     5821 ttgatctgtc ccgaatacat gggacttctg tctagatttt gccggatgat ttcgtgtgtc
     5881 ggtgcccccg tatatgtaac ataacaggaa atctgtggtt gcgtgatggc ggatgtgctg
     5941 taggaaaaag gagaaggtac tggatctggt tcttgctctt ccatcacact gaggtccaga
     6001 gattttttca acacgcgcgg aggagttcca gttttcagtc tgcctagttc aaatccgtac
     6061 gcagtcagac agtcagagag cttcatggcc gattgttccg caattctccc ggatcttgcc
     6121 tgatactctc caatgtgaat gagtccctga agaaaagttc ctgtagtcag aatcacatag
     6181 tcgcattgta tttcctggcc gcgactgcag atgatcccac aaactttatg gttctcaatg
     6241 ataagtgaat cacaagtatc ctggcggaag cttagattgg gagttgcttc cagggtccac
     6301 ttgatatggt tttgataggc ttttttttct gcctgtgccc gcggtgccca cactgcgggt
     6361 cctcgtgaac gattcagcat tttgaaatgg attccagtct ggtcgatcac acggcccatg
     6421 attccaccaa gcgcgtccac ttcgcgcacc atgtgtcctt tcgcgattcc tccgatggca
     6481 ggattgcagg acatctggcc gattgtgtcc agattcatgg tcagaagaag agtgcgtagc
     6541 ccactacgtg cacaggcatg cgccgcttct gctccggcat gtccgccgcc cacgactaca
     6601 acgtcgaaac gggatggaac tggattcgcc atacggtgta caagctacgg gaaagccctg
     6661 gccccgtcca gtctaagagc tttgcgctcg acaacgccag gagcaatgat tgcctgagtt
     6721 caccctatgc gttatacgat caaaaatctt gtgtttaagg gcggcggggt gctgggcgtt
     6781 gcatacgctg gtgccgtcga agtcctgtcg cgtgctggca ttttagcggg agtggagaat
     6841 ctggcaggca cctctgcggg agcgattgca agtctactct tgtctctgcg atacgatgaa
     6901 gaaacactca agaagatttt gctcgatctc gatttcaaac agttcacttc caaagcagag
     6961 ccacttcgct tccaggatcg atacggatgg cattctactc agccagtgcg tgaatggctc
     7021 gaagatcagg tacgcaaatc gcgccagccg ggagaacttt caggccgcga aacatttcag
     7081 gatttcaaag accttggttg cagaaatatg cacgtgtttg cgacaaatct aaatacgcga
     7141 agcgtggagg agttcagtgt tcgcaggact ccagaagtcc gtgtcgtgga tgcggtgctt
     7201 gcatctcttt ccatcccggc atactttcaa tcattccaat ttccagatgg aaaaccaaat
     7261 tcgcacatct acgtggacgg cggcgccctc ttgaattacc ccatcactgc gttcgatact
     7321 ccggacaaaa atcgccgcgg cgctctcaaa gcaaatccgg atacaattgg atttcacctg
     7381 gagcttctgg gccacgaatt tccaacgagc gatctggaat tcggaacgtt cagtaaatgg
     7441 gcgcagactc tttacgaaac agtgaaggac gtgcaatcta cgttgctgtt atcttcgccg
     7501 gagcatatgg aaagaaccgt cttcattgac aacggaggga tttctcccat cgactttgag
     7561 ataacgccag aacagaagac ctggttgatt gagagcggac ggcaagccac ggaggcctac
     7621 cttcagctgt atagatatag aaagagtttt aggtcccgcg cgttgagatc cttgatgcgg
     7681 tggagaacaa ggcgctgatt gcgtagaagt accctgtctc tggcccggtt ttctgcttgc
     7741 tgtgacacag gacggggcga gtatgaatta cgatggctgc gccagaaaaa cccagggaga
     7801 aattcagact actgaattca atgcgcattg cgctctctct ttttttcctg gcactcgcac
     7861 tgcgcgaatc gcatccgaac tttctgtact tcattgtagc acatctggtt ttctcgctcg
     7921 tgtggattct gttcgttgaa atggatcgac tgccgcgtgg aagatggatt ggatacatcc
     7981 ccgcaactct agacattggt gcggtgaccg ttgcgctgtt attgaccggg catgctcgat
     8041 cgcctctggt cgcaggttac ttcgggctgg ctgctatttc ttctactctt caaaatcgaa
     8101 catttggtct gtatgttgcc tgtctctctg tgatttcgta tgcggcagcc agtgccggcg
     8161 ttacattcgg atggctcact tccatagatg ttctggcaga aggaccacag actatttcgc
     8221 ctgcgggcat tctcgtctac acgttgctgg tggcgattgg gcttacgatc gttcacacag
     8281 tttgttttca atttgtaaat tcgaatcgca gaatattagg agaagcgaat cttctgctgg
     8341 aaaaagtcgc gatgcaagag gagcagctgc gtttgcgcaa cgcgcagatg gaacaggacc
     8401 tggatctggc gcgaagcatt cactttcgcc ttctgccaga aagccttccg gaaattccag
     8461 gacttacatt tgcaacgctc tatgttcctc aag
//
LOCUS       DAOQJL010000105         8380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4585_length_8380_cov_4.383784, whole genome shotgun sequence.
ACCESSION   DAOQJL010000105 DAOQJL010000000
VERSION     DAOQJL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4585_length_8380_cov_4.383784"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PLG78_06450"
     CDS             complement(<1..1230)
                     /locus_tag="PLG78_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22786.1"
                     /translation="MIPHTFSILEAGEKLVVDLPWCRSNPEEVHEKTRILTQDMQYRL
                     TFDGKIIKEGKVGRDFRIEVDLRGQKSGVYTMQVMGNGTTDGGVREFWKQHIFVQTSS
                     RKTPEQIREIANRYAPIFLFSKKEQYFPVALGDLIHSPELLKSNGKISVDSIAGELHV
                     PITELDEFLRHNGHADYLLNQSVFDLDGVFKEIHGDFRRSVVYYSYIEDKNSDRFFIN
                     YHTFYAFDPKTGIAKMLNIGPHVFDRESLTMVFRGDGTPESLVLSGHLENQPILFLEK
                     LMIWSTGRVRIPAPDPYGPSVDTHPIVPVAEGSHALYPTAGLYHISLLTELAGHVFRN
                     ILQMDTDDDDPREMDTHQVLLPPTMPSDKFANYDLRPLRFDLMRSEPGSASPLYDPSV
                     CALVFSGYWVDVPGLHNE"
     gene            complement(1232..1462)
                     /locus_tag="PLG78_06455"
     CDS             complement(1232..1462)
                     /locus_tag="PLG78_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE22787.1"
                     /translation="MHFSGTKIRALHEERKWTQKELAEKIALTGHFSGFTHQRIDRLE
                     KGDNASHRVLCALCEVFRVRPGVFFEDGDSAT"
     gene            1563..3719
                     /locus_tag="PLG78_06460"
     CDS             1563..3719
                     /locus_tag="PLG78_06460"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE22788.1"
                     /translation="MIELEYHGKVRIDRDQNGIPHITADTESGMYFGQGYAQAMDRGR
                     QILLMRILSQGRASEILESSDEMLEIDRFFRRMNWYGDSAQIAGQLDAATSKLAQAFC
                     DGINAFLSKKLPFEFRFLIGLKYELWEIDDSITLARMIGYISLQQSQTEVERLFVQMV
                     QAGIDRKLLEEFFPGSLDHLDEKVIRQVKLSERVVPAALQWSRIIPKVIASNNWVVAG
                     SRTKSGKPILSNDPHLEINRLPNVWQEIILTFAGRTACGVSMPGLPGILIGRNPDVSW
                     GATYTFMDAVDSWVEECRQGSYKRSQFLRVQWVRFKERREIIKRKKKPDHLEIFYENE
                     HGVLDGDPGEEGFYLATRWASGSGTGARSLEAILSMFHVTGVKQAMDTMGRLEMSFNW
                     VLADKQGNIGYQMSGLMPKRQKNWSGLPPMPGWDSDFDWKGFHSHMDLPRRYNPKEGY
                     IVTANNDLNAWGNAAPINAPMGSYRADRIEKLLKRSELTVEDMQKMHMDVYSLQAERY
                     MEILRPTLPASNPNAEILRGWDLRYDLDSKGAYIFEKFYEFLLEEVFGSVFGKQVWTH
                     IRTETGILTDFFGNFDRILLSRKSKWFGSKSRDDLFSAALTRALSEEVKAYGSGQNLI
                     LKHIILGGKFPLFFRIDRGPVGLPGNRATIHQGQIYRSAGRDTSFAPSYRMVADMNEP
                     GLNTSIIGGPSDSPFSRLYANDVDNWVRGLLKKVGF"
     gene            complement(3846..4364)
                     /locus_tag="PLG78_06465"
     CDS             complement(3846..4364)
                     /locus_tag="PLG78_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNE22789.1"
                     /translation="MPRYVAFLRGVSPMNASMAELRTRIESAGFTDVKTVLASGNVVF
                     NSRSASNESLQKKIEAAIAAGRGRPFSTIIRSVESLESFLEEDPFAQFKLPAKAKKVI
                     TFLREANPNPPALPIKRDDVFILTMSKGEVFSAYVHDPKKGPIFMQMLEKNFGKDITT
                     RTLDTVKKCVSA"
     gene            complement(4407..5009)
                     /locus_tag="PLG78_06470"
     CDS             complement(4407..5009)
                     /locus_tag="PLG78_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22790.1"
                     /translation="MSALQNTMSWSARNFIFLLAIALALLVTCSPPQAICYIVNKYDK
                     PVTAKVTFPSRHIFEIFRDAEFTLTDAKHEKYTGDRAKASADENKLILEITVPPHSAV
                     AISNLGGHSYALNRHCSEILEKAPNSEEGDRSSYEHGCESLIYRAASLQVDTPDGTIQ
                     LRGLQIAMAFRRQEALAYVLELDPDLPEFTPKEPRLCCCL"
     gene            complement(5011..5838)
                     /locus_tag="PLG78_06475"
     CDS             complement(5011..5838)
                     /locus_tag="PLG78_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE22791.1"
                     /translation="MKRISILLLVGTTAIALLFSSATAQNKQWKPGSRAPQAGDEYVN
                     PKDGTVLVFIPGGEFTMGSDSGDADEKPAHKQTVQGFWLGKYEVTNQQYQKFRDAEGY
                     PDPQAMDDPAFNAPNQPVIGVKFMHAQSYVKWAGLRMPTEAEWEYAASGGKNLKYPTK
                     TGEINHDLANYLGKEGADKWEDVPSPVGSFPPNPFGIYDLAGNAWEWTSSMYRNYPYS
                     ATDGRENQTDRRHRVMRGGTFHFGEKYIRTTHRHHFAMHLRLDYAGMRMAASTDQLK"
     gene            complement(5835..6935)
                     /locus_tag="PLG78_06480"
     CDS             complement(5835..6935)
                     /locus_tag="PLG78_06480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE22792.1"
                     /translation="MKKLTLALIFLIVPLFADEVPDQDLDVPYEPSHPKVVEAMIKAA
                     AVTGQDTLFDLGCGDGRIVIAAAKLGARAIGYDIDEQRLMEARERAHVAGVESKVEFR
                     REDIFKADLSKATVVTMYLLDEINLQMRPRLFRMLKPGTRVVTHAFHMSDWKPDSFIE
                     NERARDHKIMYWVIPASAGGTWTWKAGGQDYRMDLIQKFQVVTGTIRSGNSSANIETK
                     INGKSFTVDATLRGGKETKVSIKGMIDGDRMTGNVETAGVRSALDAKRTLTNPEGKWR
                     IEMAETKKVFSGNLTLVKDAAGWTAEFEADKRYAITDLYVWGDSVYFRVPILGAENPA
                     IYTGRLQNEGTGFLTNDAASFTTSFTVKKAAK"
     gene            7021..8298
                     /locus_tag="PLG78_06485"
     CDS             7021..8298
                     /locus_tag="PLG78_06485"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNE22793.1"
                     /translation="MGQASQEFVAEAARAGRAARHAFRILRSSSSAMRNHALLILAES
                     LESDSVQQNVFAENQKDLAAALANDLKASLVDRLKLDVKRMKEMASAVREIAQFPDPV
                     GEVIRGRTLKSGVRMIQKRVPLGVVFTVFESRPNVTVDIGALCVKSGNAAILRGGKEA
                     IHTNIALHSIFQDALVKAGLPAESIQIVTETDRELMLAFLHQDQLIDLVVPRGGEALI
                     QFTVENSRIPVVKHDRGVCNLFVDQNADFDLVLRVVANAKLQRPSVCNAIENLLVHRA
                     YPHTKKLLMGLEQEGAILLGDQESRDTYEGVGEIKNPDAEYAQEYLDNRLAVKMVGSV
                     EEAVEFIYKYGSGHSEAILSRDVEAIDRFVASVDSAAVFVNCSTRFHDGGQMGMGAEV
                     GISTGRLHVRGPMGVEHLTTTTYVMEGEGQVRL"
BASE COUNT         1950 a         1966 c         2195 g         2269 t
ORIGIN      
        1 ctcattgtgg aggccgggaa cgtctaccca gtatccactg aacacaagtg cacacacgga
       61 aggatcgtac agcggggaag cagatcctgg ttcgctcctc attaaatcaa aacgaagtgg
      121 ccgcaaatcg tagtttgcga atttgtccga tggcatagtc gggggcagta gaacttgatg
      181 agtgtccatt tcgcgcggat cgtcatcatc cgtatccatc tgtaaaatgt ttctaaagac
      241 atggcctgcg agttctgtaa gaagagaaat gtgatagagt cctgccgttg gataaagagc
      301 gtgggatcct tctgcaaccg gcacaatcgg atgagtatcc actgagggac cgtaaggatc
      361 cggcgccggg atgcgcaccc ggccagtgct ccagatcatc agcttttcca gaaacaggat
      421 gggctgattt tcgagatgcc ctgatagaac cagagactca ggcgttccgt cgccgcgaaa
      481 caccatggta agactttctc ggtcaaagac atgcggccca atgttgagca tctttgcaat
      541 gcccgtcttt ggatcaaatg cgtagaacgt gtggtagttg atgaagaatc tgtctgagtt
      601 tttgtcttct atgtaggagt aatagacaac ggatcgcctg aagtctccgt ggatttcttt
      661 gaacacccca tctaaatcga aaacggattg attcaacaga tagtctgcgt gcccgttgtg
      721 tcgcagaaat tcgtctagct ctgtgatggg gacatgcagt tcccctgcga tagaatctac
      781 agaaatcttt ccattggact tgagaagctc aggggagtgg atgagatctc cgagtgcaac
      841 cggaaaatac tgctctttct tgctgaacaa aaagatgggc gcataacggt ttgcgatttc
      901 ccgaatttgt tccggtgttt tgcgcgatga tgtttgaaca aatatatgct gtttccagaa
      961 ttcgcggact cctccgtctg tggttccgtt tcccatgact tgcatggtat agacgccgct
     1021 cttttgaccg cgcagatcca cctctatgcg aaaatcgcgt ccgactttgc cttctttgat
     1081 gatctttcca tcaaacgtta ggcgatattg catatcctgt gtgaggatgc gagttttctc
     1141 atgaacctct tccggattgc ttctgcacca cggcaggtcc accacaagct tttcgcctgc
     1201 ttctaagatg ctgaatgtgt gaggaatcat attatgtcgc agaatctccg tcttcgaaga
     1261 atacacctgg ccgcactcta aagacttcgc acagcgcaca cagcactcga tgggaggcat
     1321 tgtctccttt ttcgagtctg tctattcttt ggtgtgtgaa gccactaaaa tgtccggtga
     1381 gtgcaatctt ctctgcgagt tctttctgag tccactttcg ctcctcgtgc agggctcgga
     1441 tttttgttcc gctgaagtgc atatcccccg tctgaagttg agtgattttg acgaaattgt
     1501 aaagagaaat tttctcgcaa atttgcggat cgttccgcct gctgggattc ggagccaaag
     1561 acatgatcga actagaatat cacggaaaag tcaggattga ccgggatcag aacggaattc
     1621 ctcacataac agcagacacg gaaagcggca tgtattttgg ccagggctac gctcaggcaa
     1681 tggatcgcgg aaggcagatt ctactgatgc gcattctttc tcagggtcgc gcttccgaga
     1741 tactcgaatc ctcggatgag atgcttgaga ttgatcgttt ctttcggagg atgaactggt
     1801 acggtgacag cgctcagatt gcagggcagc tggacgcggc cacatctaaa ctcgcgcagg
     1861 ctttttgcga cgggatcaat gcatttcttt ctaagaaact cccatttgaa tttcgattct
     1921 tgattggttt gaagtatgaa ctctgggaaa tcgatgattc gatcactctg gctcgtatga
     1981 tcggttacat cagtcttcag cagtcgcaga cagaagtaga acgattgttt gttcaaatgg
     2041 ttcaggctgg aatcgatcgc aagcttctgg aagaattttt tccaggaagc ctggatcatc
     2101 tggatgagaa agtaatccgt caggtgaaat tgtcagagcg agttgttccg gccgcgcttc
     2161 agtggagtcg catcattcca aaggtaatcg catccaacaa ctgggtcgtg gctggttctc
     2221 gcactaaatc cggaaaaccg attctcagca atgatcctca tcttgaaatc aatcgccttc
     2281 cgaatgtgtg gcaagagatc atactgacgt tcgcaggtag aactgcatgc ggcgtttcga
     2341 tgccaggtct tcccgggatt ttgattggcc gcaatccgga tgtgtcgtgg ggcgcgacgt
     2401 acactttcat ggacgcggtg gattcctggg tagaagaatg cagacaggga agctataagc
     2461 gcagtcaatt tttgcgcgtg cagtgggtga gattcaaaga gcgccgcgaa atcatcaaaa
     2521 gaaagaagaa acccgatcat ctcgagatct tctacgagaa cgagcatgga gttctcgatg
     2581 gcgatccagg agaagaaggt ttctatctgg cgactcgctg ggcctcaggt tctggaacag
     2641 gagcaagatc gctcgaagca attctttcta tgtttcacgt gaccggagta aagcaggcca
     2701 tggacacgat gggcaggttg gaaatgtcgt ttaactgggt gttagctgat aagcagggaa
     2761 acattggtta ccagatgtcc ggtctcatgc ccaaaagaca gaagaactgg tccggtcttc
     2821 ctcccatgcc tggctgggat tctgattttg actggaaagg ctttcactct cacatggatt
     2881 tgcctcgtcg ttacaatcca aaagaaggat acattgtaac ggcaaacaat gatctcaatg
     2941 cgtggggaaa tgcagcgccc atcaatgctc ccatgggaag ctatcgcgcg gatcgaatcg
     3001 aaaaactact caaacggtct gaactcactg tagaagacat gcagaaaatg catatggatg
     3061 tgtattctct ccaggcagag agatatatgg aaatcctgag gcctactctt cccgcgtcca
     3121 atccgaatgc ggagatcttg cgcggttggg atctgaggta tgatctggat tctaaaggtg
     3181 cctatatatt tgagaagttt tatgaattct tgctcgaaga agtgtttggt tccgtttttg
     3241 gtaaacaggt ctggacccat atccgcacag agacagggat actcactgac ttctttggga
     3301 acttcgacag aattctttta tcgcgcaaat ccaaatggtt tggatcaaag tcgcgagacg
     3361 acttattctc cgcggcactc acgcgtgctc tttcagaaga agtgaaggcc tacggctcag
     3421 gacaaaatct catcttaaaa cacatcattc tgggcggaaa attccctctg ttttttagaa
     3481 tcgatcgcgg gccggtgggt cttcccggaa atcgcgccac cattcatcag ggacagatct
     3541 atcgcagtgc aggtagagat acaagctttg cgcccagcta tcgtatggtg gcagatatga
     3601 atgagccggg cttgaacact tccatcatcg gagggccatc tgacagtcca ttctccaggc
     3661 tctacgcgaa tgatgtggac aactgggtgc gggggctttt gaaaaaagtg gggttctgaa
     3721 aacagagggc gtgggaattg cgcgggccac gaattgacgt taggcggcgt gggatgaaga
     3781 aatttcgcac tgcgaaattc tcctatggga agtgcgctgt attttgaccg catgcgaatc
     3841 gcccgctatg cggaaacgca tttcttcact gtgtccagag tgcgagttgt gatgtccttt
     3901 ccaaagttct tttccagcat ttgcatgaag atcggccctt ttttcgggtc gtgcacataa
     3961 gcagaaaaca cttccccttt tgacatggtg agtatgaaga catcatcccg tttgataggg
     4021 agcgctggag gatttggatt tgcttcgcgc aaaaaggtga tcaccttttt tgctttcgcg
     4081 ggtagtttga actgtgcgaa aggatcctct tcaaggaatg attccaggct ttccacagaa
     4141 cgaatgattg tgctgaaggg tctgccccgg ccggccgcga tggcggcttc gatttttttt
     4201 tgcaggcttt cgttggatgc gcttcttgag ttgaaaacta cgttgccact tgccagcaca
     4261 gtcttcacgt ccgtgaagcc tgcggactct atgcgcgttc ttaactcagc catactggcg
     4321 ttcatcgggc ttactccgcg aagaaatgca acgtagcggg gcataagtca ctccagggga
     4381 aaagaatgta ttcgaaacga ttgcttctag aggcagcaac acagacgagg ctcttttggt
     4441 gtgaattcag gtaaatccgg atcgagctcg agtacgtaag cgagagcttc ctgccggcga
     4501 aatgccatgg cgatctgcag tccccggagc tggatcgttc cgtctggagt gtccacttgc
     4561 aaggaagctg cccggtagat cagagattca cagccgtgtt cgtaggacga tcgatctcct
     4621 tcttcagagt ttggtgcttt ttccagtatc tcagaacaat gtcgattcag tgcatacgaa
     4681 tgcccgccca gattggagat ggcaaccgca gagtgagggg gcaccgtgat ttccagaatg
     4741 agtttgtttt catccgcgga tgctttagcg cgatcgcccg tgtacttttc gtgcttcgcg
     4801 tctgtcagtg tgaattcagc gtctctgaaa atctcaaata tgtgacggct cgggaaagtt
     4861 acttttgcag tcacaggttt gtcgtatttg tttacgatat agcagattgc ctgaggtgga
     4921 gagcatgtaa caagcaacgc aagggctatc gccagcagga aaatgaaatt tcgcgcgctc
     4981 cacgacattg tattctggag cgcgctcatt ctatttcaat tgatctgtag atgcggccat
     5041 tcgcattcca gcataatcca gcctgaggtg catcgcgaaa tgatgtcgat gcgtagtgcg
     5101 aatgtatttt tcgccaaaat gaaacgttcc gccccgcatc actctgtgtc tgcggtccgt
     5161 ctggttttcg cggccatctg ttgcactgta cggatagttg cgatacatgc tcgaagtcca
     5221 ctcccaggca ttccccgcaa gatcatagat tccaaacgga ttgggaggaa aacttccaac
     5281 aggtgacgga acatcttccc atttgtccgc gccttctttt cctagatagt tcgcaaggtc
     5341 gtgattgatt tcgcctgtct tcgtgggata ttttagattt tttccgccgg aagctgcata
     5401 ttcccattca gcctccgttg gcatgcgaag gccagcccac tttacatagg actgagcgtg
     5461 catgaacttc actccgatca caggttgatt gggggcattg aacgcaggat cgtccattgc
     5521 ctgtggatcg gggtaacctt ctgcatcgcg gaatttttga tactgttgat tggtgacttc
     5581 atacttaccc agccagaatc cttgcacagt ctgtttgtgc gcgggttttt catccgcgtc
     5641 gcctgaatcg gagcccattg taaattcacc gcctggtatg aagaccagaa cagttccgtc
     5701 ttttggattt acgtattcgt ctccggcctg aggtgctcga cttcctggtt tccattgttt
     5761 gttctgggcg gttgcgctcg aaaataaaag ggcgatagca gttgtgccga ctaaaagcag
     5821 tattgagatt cgcttcattt cgcggccttc tttacagtga agctggtagt aaaggaagca
     5881 gcatcatttg tgagaaatcc cgttccttcg ttctgaaggc gtcctgtgta gatggcagga
     5941 ttttccgcgc ccaggatagg tacgcgaaag taaacgctgt ccccccagac gtagagatct
     6001 gtgatcgcat aacgtttgtc cgcttcgaac tctgcagtcc atccggctgc gtctttcacc
     6061 agagtgaggt ttccgctgaa cacttttttt gtttccgcca tttctattcg ccactttcct
     6121 tccgggtttg ttagcgtcct tttggcgtcg agcgcgcttc gcacgcctgc agtctcgacg
     6181 ttgccggtca tccggtctcc atcaatcatg cctttgatag atactttagt ttctttacca
     6241 cctcgcagtg tcgcgtcgac tgtgaaagat tttccgttga tttttgtttc aatgtttgcg
     6301 gatgaatttc cgctgcgaat cgtgcctgta actacctgga acttctggat gagatccata
     6361 cggtaatctt gcccgcctgc tttccaggtc caggtgcctc ctgccgacgc agggatgacc
     6421 cagtacatga ttttgtgatc tcgcgctctt tcgttttcaa taaatgagtc cggcttccag
     6481 tcagacatat gaaatgcatg agtgacaact ctggttcccg gtttgagcat gcgaaataga
     6541 cgcggcctca tctgcagatt gatttcatcg agcagataca tggttacaac cgttgccttt
     6601 gaaagatctg ctttgaaaat atcttcgcgc ctgaactcaa ctttgctttc tactccggcc
     6661 acatgtgctc tttcgcgcgc ctccatgagg cgttgttcgt ctatgtcata accaatggcc
     6721 ctggctccca gctttgcggc tgcaattacg attctgccgt ctccgcaacc cagatcaaat
     6781 agagtatcct ggccggtgac tgcggccgcc ttgatcatgg cttctactac cttcgggtgg
     6841 gaaggttcgt atggaacgtc gagatcctga tcgggcactt catctgcaaa aagaggcacg
     6901 atcaggaaga tcagggccag agtgagtttt ttcatatgtt ttgcagattt tggacgcagg
     6961 cggcattgat caaggctgaa tttttctttg cactgccgga acaaaatcca tcccgtccat
     7021 gtgggccagg caagtcagga atttgttgca gaagcagcca gagcgggtcg agcggccagg
     7081 catgcgtttc gcattttgag atcctcctct tctgccatga ggaatcatgc tcttttgatc
     7141 cttgctgaat cacttgaatc ggattctgtt caacaaaacg tattcgcaga aaaccagaag
     7201 gatctcgcag ccgctctcgc aaatgatctc aaagcttctc tcgtggatcg tctgaagcta
     7261 gacgtgaaga gaatgaaaga gatggcttct gccgttcgcg aaattgcgca atttccagat
     7321 cctgtgggtg aagtcattcg cggtcgcact ctgaagagtg gggtgcgcat gatccagaag
     7381 cgcgttccgc tcggagtagt cttcacagtc tttgaatcgc gtcccaatgt gactgtggac
     7441 attggcgcac tctgtgtgaa aagcggcaac gctgccattc ttcgcggagg caaggaagcc
     7501 attcacacga acattgcact tcattctatt tttcaggatg ctctggtaaa agcgggcctg
     7561 cctgcggaaa gcattcagat tgttacggaa acggatcgcg aactcatgct tgcgtttttg
     7621 caccaggatc agctgatcga tctggttgtg ccgcgtggcg gggaagctct gattcaattt
     7681 accgttgaga attcgcgcat tccagttgtg aagcacgacc gcggtgtgtg caatctcttc
     7741 gtagatcaga atgcagattt cgatcttgtt ctgcgcgtgg ttgcaaatgc gaaattgcaa
     7801 aggccttctg tgtgcaatgc gattgagaat ctgcttgtcc atcgtgcgta tccgcataca
     7861 aagaaactac tcatgggcct tgagcaagaa ggagcgattt tgcttggcga ccaggagtcg
     7921 cgcgatactt acgagggcgt gggggaaatc aaaaatccag acgcggagta tgcccaggaa
     7981 tatctggata acaggcttgc tgtgaagatg gttgggtctg tggaagaagc agttgagttt
     8041 atttataaat atggcagcgg acattcagaa gcaattctgt ccagagacgt ggaagcaatc
     8101 gatcgatttg tagcaagcgt ggactctgcg gccgtgtttg tgaattgctc cacccgcttt
     8161 cacgatggtg gccagatggg gatgggcgca gaagttggaa tttccaccgg aaggttgcat
     8221 gtgcgaggcc cgatgggagt ggagcatttg actacaacca cctatgtgat ggaaggcgaa
     8281 gggcaggttc gactctagat gtttagagtc tttttgagtt gagccagaga gtttgcagct
     8341 cttactttcc ctgtttttgt ttttccaatc gtgtgtgtgc
//
LOCUS       DAOQJL010000106         8377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4590_length_8377_cov_4.679644, whole genome shotgun sequence.
ACCESSION   DAOQJL010000106 DAOQJL010000000
VERSION     DAOQJL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4590_length_8377_cov_4.679644"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLG78_06490"
     CDS             complement(<1..213)
                     /locus_tag="PLG78_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22794.1"
                     /translation="MKTQSQMGLRESLRNRFRSARSLDPAILRILDDESLRNAKALNS
                     FRVFGAAVWVLLAMVLGYWKNQSDWII"
     gene            complement(225..791)
                     /locus_tag="PLG78_06495"
     CDS             complement(225..791)
                     /locus_tag="PLG78_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNE22795.1"
                     /translation="MPDESMPRSVFLKKAGTTFLLLTTSGTVLSTLHCGTHMARDLRF
                     KNLDDALLEIDRVSVSAKVETNGPWNSSQVFAHLAQSIEYSMTGYPENKPAIFRKTIG
                     RIVLAKFLGQGYMQHNLSDAIPGAPALDANLPFPDAVARLKKAISDFRAFQDHLAMHF
                     VYDEATKDQYERVHAYHIANHLSAFTIG"
     gene            complement(844..1338)
                     /locus_tag="PLG78_06500"
     CDS             complement(844..1338)
                     /locus_tag="PLG78_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE22796.1"
                     /translation="MMLLVDAFNVIYKFPVLEEHMAQGRLAEARRGLLEIVYDFAKKW
                     KKPLDIHLVFDGKKNKGDATESEIVSGIKVIYSHDLSADHIIKQFIKHNARPADIRVV
                     TSDKDILGHAKKWHCDRMTSEDFHKWVVSTLEDKPLPEEKPQTEDVDVGYWMSMFKNR
                     RPGK"
     gene            1386..1808
                     /gene="hemJ"
                     /locus_tag="PLG78_06505"
     CDS             1386..1808
                     /gene="hemJ"
                     /locus_tag="PLG78_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HNE22797.1"
                     /translation="MILWLKAFHIIGFTAWFAGIFYIWRLFVYHAETTSEDVRNQLAI
                     MEYKLYKYIMRPAMVVTLFFGFALLYLQWEAFFRSAWIWLKLCLVALVLANHFMAESY
                     RKKLARGMAYKSRIFRILNEMPTVLLIGIVLLVVLKPF"
     gene            1847..3499
                     /locus_tag="PLG78_06510"
     CDS             1847..3499
                     /locus_tag="PLG78_06510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE22798.1"
                     /translation="MKFSELHIHPDIIRACTDAGFETLTEIQEKCLIPATQGRDIAGI
                     SQTGTGKTVAFLLPLLDRIVKDNLPGPAALIITPTRELCLQIAEEALKLTKHGPVSVS
                     AVYGGEGYRKQEEELAKNPQIIVATPGRLIDYIKQGKIDLNKLHFLVLDEADRMFDMG
                     FIRDIGFIMKKVPREAQTMLFSATLSYYVMRLAADFMNDPVEVRIEAETVAVDKIDQQ
                     LFHLGKDEKMPYLINQVLAVPGIRAIVFTNLRVYVSTIVNNLRRYGVHATGLSSMLDQ
                     KKRVRLLKDFKLGKYSVLVATDVASRGLDVDDITHVFNYDLPQDAESYVHRIGRTARA
                     GRSGISISYCSEMDYEALPRVESYLGKKIPVKTVDVELLKFPAGQFTPFQEAHGERVE
                     GEDRSDRDSNRDGRGGGRNRGGRKRKGQDRPGFAPARGRDRNDQDPSKEGGARAGVNS
                     DRNQKDDRGRRDDRGRRDSRDRRDDRGRRDGHPGQDRGRNDRHGQGNRGRDGGRPGRG
                     DGRHGSHRHRDRDRTRRESVPQKKPGIIARILSIFKKKKPEA"
     gene            complement(3524..4198)
                     /locus_tag="PLG78_06515"
     CDS             complement(3524..4198)
                     /locus_tag="PLG78_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22799.1"
                     /translation="MSQCRLTFLKTGLSFVFLFFTVGLYADPPEWRKDMPWFDNPKED
                     YRAYGPDDIKPYLEKPKERSRSGFALPDLTQLVYAVAAILAGAAIYLIVRRILERRPA
                     EEEVLEETPEMKVSVVVDLPEAHLPDQDLVKVIEQLLAAGNFRRAGMLLFQLACNRLK
                     AEGLLDFHEYQTPREIASTLNTEREGEILAQAGKVFEDCAYRPEEPSGAAVISLKERV
                     FSLGPA"
     gene            complement(4201..5025)
                     /locus_tag="PLG78_06520"
     CDS             complement(4201..5025)
                     /locus_tag="PLG78_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22800.1"
                     /translation="MNLLDLRIPLRRRRLDEIFDLAVIVLKSNHWRYGRILFPFTISC
                     IAINYGVTKLVKEDVLANGMIVLLLLFESSVLQMIFNALNGRIVFELKPRFRDVLADT
                     KQTAPVYIGRMAILNTLYYTLLFPFIIPPVRALFTHFFMGEVIILERLRGAPLGLRAA
                     ALARGQGDRIVGFFVFSCLMFLAGLVSTVYGLSSMRELMGGWAEKNIPSPGFAPTGVV
                     FQLVFFPSIAYFCLVRFLLYLDTRIAGEGWDIELLLARGIQETQQEPEARYERRSA"
     gene            complement(5022..5969)
                     /locus_tag="PLG78_06525"
     CDS             complement(5022..5969)
                     /locus_tag="PLG78_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNE22801.1"
                     /translation="MTPAIFIEQRSKDWDDLEKMLPDLERKFRLPVREILKFARLYRA
                     VSTDLSVAQAFRLSRMQQARLEGLVSRGHSVLYSIPRRKLTDVRRYIFETVPAAIRKD
                     VYVRICLLVFYVPFFLCAGMAYVSRPFAAQVVGEAVLEEYHDMHEGERGEIKLGDLVG
                     ASGFYIANNIGLNLITFGLGALVGIGSLFFTLYNAVFLGTIIGYLMSTPARDHIVSWA
                     AAHAPFELTAIGLSAAAGVRIGFCLIAPGARSRLLAIREESLTAMPALYSAAMLTFFA
                     AFIEAFLGPWNPGVNVKLALGGVTTLLTLAYFLAGGRGK"
     gene            complement(5979..6761)
                     /locus_tag="PLG78_06530"
     CDS             complement(5979..6761)
                     /locus_tag="PLG78_06530"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNE22802.1"
                     /translation="MQNRNLDSVLEIPAPEFIVFRAQTAGPMARFMAVFLDHAIIFLL
                     LGITAFFAAIFAAFELAGTKSAGFSTFLLLIAFFFLQWFYFFAFEWLNKGRTPGKMAL
                     GLRVISIDGTAVDVVQILIRNMLRIADMFPFKFLVWLLYLPTYTIGLISSVMSAPRFR
                     RLGDLAAGTIVIREIPTEKEKAITVRVPEDLVRKISFRILPSATLVQALHDFASRYEK
                     MHPARRMEIADRVRPGLEWLAGHQECTSEQLLLACHRKLTED"
     gene            complement(6751..7236)
                     /gene="coaD"
                     /locus_tag="PLG78_06535"
     CDS             complement(6751..7236)
                     /gene="coaD"
                     /locus_tag="PLG78_06535"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNE22803.1"
                     /translation="MKTAVYPGSFDPFTNGHLDLVQRASQLFDRVIVSVAINSSKNSF
                     FTVEERMAILREVLASMPGVEVASFEGLLVDYCRTVGARAIVRGLRAVTDFDYEYAIF
                     QLNHDLNAEVETVFLLAAKEYSFVSSTLIKEVARYGRSVEQHAPPAVSRALLRKFGHA
                     K"
     gene            complement(7252..>8377)
                     /locus_tag="PLG78_06540"
     CDS             complement(7252..>8377)
                     /locus_tag="PLG78_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE22804.1"
                     /translation="GGIASILVGAFTDIWNRKLLLFGTVFAGELACFFTAFAPNYTTY
                     IVLRTLTGIGLGGIFPIIFSLLGDHFKPENRSSASGWLALAMGLGIGVGQILGGMLAT
                     ATVFGLEGWRASFAIMAGPCFPLLFLYLAIGETPKRGGAENVHETTGEEPKHSLGLSD
                     FKRIASSKTNILAILQGIPGCVPWGLIFFFLVDYYEKSKGFKPSDGVLLTTVFGGVLI
                     FGGFIGGFVGRAVYNWKKAALPVFCAACVLLGIVPMIYILNFQGNSMLYPMIAAALGG
                     IVIPQAGPNIRSILVNTNLPENRGSIFGVFNFADDLGKGLGPFVVGEILRLVGSDSLA
                     YNLAIYCWIPCAIGWLLMAFTMEADEKKVEDTLIARGAKR"
BASE COUNT         2146 a         2079 c         2083 g         2069 t
ORIGIN      
        1 gatgatccaa tcgctttgat ttttccagta tcccagaacc atagccagca gtacccacac
       61 ggcggctccg aaaacgcgaa agctgttgag tgcctttgca tttcgcaggc tttcgtcgtc
      121 tagaatgcga aggatggccg gatcaagaga acgcgcgctg cgaaacctgt ttcgcaagga
      181 ttctcgcagt cccatctgcg attgagtttt caaaatctgt tccgtcagcc aatcgtaaac
      241 gcggacaagt gattggcgat atggtaggcg tgcacgcgtt cgtactggtc tttggttgcc
      301 tcatcataga caaaatgcat ggcaagatga tcctggaatg ctctgaaatc agagattgct
      361 ttcttgaggc gcgcgaccgc atctgggaaa ggtagattcg catcgagagc cggagcgcca
      421 ggaatggcat cgctcaaatt gtgctgcatg taaccctgtc caaggaactt cgcaagtaca
      481 atgcgaccaa tggtcttgcg aaagatggcg ggcttgtttt cagggtaccc tgtcatcgaa
      541 tactcgatgc tctgcgccag gtgtgcgaag acctgggagg aattccaggg cccgtttgtt
      601 tcaaccttcg cggacacgct tactctatca atttccagga gtgcgtcgtc caggtttttg
      661 aatcgtagat cccgagccat atgtgttcca cagtgaagag tcgaaagaac agtgccagac
      721 gtagtgagta acaggaaagt ggttcccgct ttcttcaaga ataccgaacg aggcatggat
      781 tcatctggca tggggttcct ccgcgcacca gaatgacacc gcgtgtttga tcgtgcaacc
      841 caatcacttg cccgggcgtc ggtttttgaa catggacatc cagtatccta catccacatc
      901 ttcggtctgt ggtttttctt ctggaagggg tttgtcttcc agagtggaaa ccacccactt
      961 atgaaaatct tctgaggtca ttctgtcgca gtgccacttc tttgcatgcc ctagaatgtc
     1021 tttgtctgag gtcaccacgc gaatgtcagc ggggcgggcg ttgtgtttga tgaactgttt
     1081 gatgatgtga tctgcggaaa gatcgtggct gtaaatgact ttgatgccag aaacgatttc
     1141 gctttctgta gcatctcctt tgttcttctt gccatcaaag accaggtgaa tgtccagagg
     1201 cttcttccac ttctttgcga agtcgtagac gatttccagt agtccgcgtc tggcctctgc
     1261 cagtctgccc tgcgccatgt gctcttcgag cactggaaat ttgtagatca cgttgaatgc
     1321 atctaccagg agcatcatgg ctttagattc acttgacgtg ccgcaggctt tgtttctggt
     1381 ttcttatgat tctctggctc aaggcgtttc atatcattgg cttcactgcc tggtttgcgg
     1441 gaatcttcta tatctggcgt ctctttgtgt atcacgcaga aacgacttca gaagatgttc
     1501 gcaaccagct tgcgatcatg gaatacaaac tctacaaata catcatgcgg ccagccatgg
     1561 tcgtcacttt gttttttggt tttgcgttgc tctatttaca gtgggaagct ttttttagat
     1621 ccgcctggat ctggttgaaa ctctgtctcg tcgccttagt gcttgcaaat catttcatgg
     1681 cagagtcgta tcgcaagaag ctggcccgcg gaatggcgta taaatccagg atctttcgca
     1741 ttctcaatga aatgcccact gtgcttttga ttggaattgt gctgctggtg gtgctcaagc
     1801 ctttttaagt ggacaggaaa gctgtttttc gcgaaatcga ctgacgttga aattttccga
     1861 attacacatc catcctgaca tcattcgggc gtgcacagac gccggttttg aaacacttac
     1921 tgaaattcag gaaaaatgcc tgatccctgc cacgcagggc cgcgatatcg cgggcatctc
     1981 acaaacggga acaggcaaaa cagtcgcatt ccttcttcct ctgctggatc gaattgtaaa
     2041 agacaatctt cctggtccgg cagctctcat catcactcct actcgcgaac tctgtttgca
     2101 aattgcggaa gaagctctga agcttacgaa gcacggtcca gtcagcgtca gcgccgttta
     2161 cgggggagaa ggttatcgca agcaggaaga agagctcgcg aagaaccctc agatcatcgt
     2221 agcaacgccc ggacgtctca tcgactatat caaacagggc aaaatcgatc tcaacaagtt
     2281 gcacttcctg gtgcttgatg aagcagaccg catgttcgac atgggtttca ttcgcgacat
     2341 tggctttatc atgaagaagg ttccgcgcga agcgcaaacc atgcttttct ccgctactct
     2401 gtcttactac gttatgcgac ttgcggcgga cttcatgaac gatcctgtcg aagttcgcat
     2461 agaagcagaa acagttgctg tggacaaaat tgatcagcag ctttttcatc ttggcaaaga
     2521 cgagaagatg ccgtatctga tcaaccaggt actcgccgtt cctggaattc gtgccatcgt
     2581 atttacgaac cttcgcgttt acgtttctac cattgtaaac aatctgcgtc gctatggagt
     2641 gcatgcaacc ggtctttcct ccatgctcga tcagaaaaag agagttcgtc ttctcaaaga
     2701 tttcaaactg ggtaaataca gtgtgctcgt tgcaacagac gtggccagcc gtggtctaga
     2761 cgtagacgat atcactcacg tttttaacta tgatctccct caagatgcgg aaagttacgt
     2821 gcatcgcatt ggcagaacag cgcgcgctgg gcgcagtggc ataagcatca gctattgcag
     2881 cgaaatggac tacgaagcac ttcccagagt ggaatcgtat ctggggaaga agatcccggt
     2941 gaagactgtg gatgtggaac ttttgaaatt tcccgcaggc cagttcactc ctttccagga
     3001 agctcacgga gaacgtgtgg aaggagaaga cagatcagat cgcgactcga acagagacgg
     3061 gcgtggcgga ggaagaaatc gcggaggccg caagcgcaaa gggcaggatc gtccgggctt
     3121 tgctccggct cgcggtagag atagaaatga tcaagatcct tcgaaagaag gtggagcgcg
     3181 tgctggcgta aattcggatc gcaatcagaa agatgatcgc ggaaggcgag acgatcgcgg
     3241 acgcagagat tcgcgtgata gaagagatga tcgcggtagg cgcgacggcc atcctggaca
     3301 ggatcgtggc agaaatgatc gacacggtca gggaaatcgt ggtcgggatg gcggtcgccc
     3361 tggaagggga gacggtcgcc atggttcgca tcgtcatcgc gatcgcgatc gcaccagacg
     3421 tgaaagtgtt cctcaaaaga aaccaggaat cattgctcgc attctttcta ttttcaagaa
     3481 gaagaaaccg gaagcttagg ttccagggtt gaatcggcct gactcaggcc gggccaagag
     3541 aaaagacccg ttctttgagt gagatgacgg ccgcgccgga tggctcttct ggtctgtatg
     3601 cgcaatcttc aaaaaccttg cctgcctgcg caagtatctc accttctctc tctgtgttga
     3661 gagtagatgc aatttctctt ggagtctggt attcgtggaa atcaagcagg ccttccgcct
     3721 tgaggcgatt gcatgccagt tgaaataaga gcattcccgc tcttctgaaa tttccagcag
     3781 ccagaagctg ctcaatgact ttgaccagat cctgatccgg aagatgtgct tccggtaaat
     3841 ccacaaccac agatactttc atctcaggcg tctcttccag gacttcttct tctgccggtc
     3901 ttctttcgag gatgcgtcga acaatgagat agatcgcagc ccctgcaaga atggcggcca
     3961 cagcatacac gagctgtgtc aaatctggca gcgcgaagcc cgatctactc cgttcttttg
     4021 gcttttctaa atatggtttg atatcgtccg gaccgtaggc gcgataatct tcttttggat
     4081 tgtcgaacca gggcatgtcc tttctccatt caggcggatc tgcgtacaga cccacagtga
     4141 agaacaggaa aacgaatgaa aggccagtct tcaggaacgt tagacgacat tgagacatga
     4201 tcaagcgctc ctcctttcgt atctggcttc cggttcctgt tgggtttcct gaatccctct
     4261 tgcaagaagc aattctatgt cccatccttc tcccgcgatt ctagtatcta aatatagaag
     4321 aaagcgtacc aggcaaaagt aggcgataga aggaaagaag accagctgga ataccactcc
     4381 agtgggagca aatcctggag atggaatatt cttttccgcc caaccaccca tcaactctcg
     4441 catagagctt aagccgtaga ctgtagagac caggcctgcc agaaacatca ggcaagaaaa
     4501 aacaaaaaag ccaacgattc tgtctccctg accgcgggcc aatgccgccg cacgcaatcc
     4561 aagtggagct cccctcagtc tttccagtat gatcacttct cccatgaaga aatgagtgaa
     4621 cagtgcgcgc accggaggaa tgataaatgg aaaaagcaac gtgtagtaca gagtattgag
     4681 aatggccatg cgtcctatgt ataccggggc cgtttgcttt gtatctgcca gaacatctct
     4741 gaatcttggt ttaagctcaa atacaatgcg tccattcagt gcattgaaaa tcatttgtag
     4801 aacagatgat tcaaacaaaa gaagaagcac gatcattcca ttggccagaa cgtcttcttt
     4861 tacgagtttt gttacaccgt agttgattgc gatgcaactg attgtaaaag ggaacaggat
     4921 ccttccatat ctccagtggt tggattttag cacgatcacc gcaagatcaa agatttcatc
     4981 caatcttcgc ctgcgcagtg gaatgcgaag atccagtaga ttcacttgcc tcttcctccc
     5041 gcaaggaagt aggcgagcgt cagtagagtg gtcacgccgc ccagggcaag cttcacgttt
     5101 actccaggat tccagggacc aaggaacgcc tcgatgaatg cggcgaagaa agtaagcatg
     5161 gccgcgctat agagcgctgg catcgcagtc aaagattcct cgcgaattgc cagaagtctt
     5221 gaccttgcac ccggtgcaat gaggcaaaat ccaattcgta ctcctgcagc tgcggacaga
     5281 ccgatcgcag tgagttcaaa gggagcgtgc gcggcagccc acgagactat gtgatctctc
     5341 gcaggtgtgg acatgagata gccaatgatc gttcctagaa acactgcatt gtaaagagtg
     5401 aagaacaagg agccaatccc aacaagcgct cccaggccaa acgtgatcaa attcaagccg
     5461 atattgtttg caatgtagaa gccgcttgcc cccacaagat cgcctaactt tatttctccg
     5521 cgttctccct cgtgcatgtc atggtattct tccagaactg cttctccaac cacctgagct
     5581 gcaaaaggtc tggatacata cgccattcca gcgcacagaa aaaaaggaac atagaaaacc
     5641 aggaggcaga ttcgcacata gacatctttg cggatcgcag ccggaactgt ttcaaaaata
     5701 tatcttctta catccgtgag ctttcttcgc gggatagaat acaggactga gtgaccacga
     5761 ctcacgaggc cttcgagtct ggcctgttgc atgcgcgaaa gcctgaatgc ctgggccacg
     5821 ctcaggtctg tactcactgc gcgatacaat cgtgcaaatt tcagaatctc gcgcacgggc
     5881 aggcgaaact ttctttccag atccggaagc atcttttcca gatcatccca atccttgcta
     5941 cgctgttcta tgaaaatcgc aggcgtcatc ttcgagtttt agtcttccgt aagttttctg
     6001 tggcaggcaa gtagaagctg ttcggaagtg cattcctggt gtcctgcaag ccattccagt
     6061 ccaggtctga ctctgtctgc gatctccatt cttcgagcag ggtgcatttt ctcgtacctg
     6121 cttgcaaagt cgtgtaacgc ctgcaccagg gtggcggaag gtagaattct aaaagagatt
     6181 tttctcacca gatcttccgg cacgcgtact gtgattgctt tttctttttc agttggaatt
     6241 tcgcgaatca caatggttcc ggccgccaga tcgcccagtc tgcgaaatct aggagcactc
     6301 ataacgctgg aaatgagtcc gatcgtatag gtgggaagat ataggagcca taccagaaat
     6361 ttgaatggga acatgtctgc gatgcgcaac atgtttcgaa tcaagatctg cactacatcc
     6421 acagcagttc catcaatgga aatcactcga aggccaagag ccattttgcc tggagtcctt
     6481 cctttgttca gccattcgaa tgcgaagaag tagaaccatt ggagaaagaa aaatgcaatc
     6541 agcaaaagaa atgtggaaaa tccggcgctc tttgttcctg caagctcgaa ggctgcaaat
     6601 atggccgcga aaaatgcagt gattccaagc agaaggaaga tgatggcgtg atcgagaaac
     6661 acggccatga agcgtgccat cgggcccgcc gtttgcgcgc gaaatacaat gaactcgggt
     6721 gcagggatct cgagaacgct gtccagattt ctattttgca tgaccgaact tgcgaaggag
     6781 tgcccggctg actgcaggag gtgcgtgttg ttctacactt cttccgtaac gcgccacttc
     6841 tttgatgagg gtgcttgaaa caaaggagta ttcttttgcg gccagcagaa acaccgtttc
     6901 cacttctgcg tttaagtcgt ggttgagttg aaagatggcg tattcgtagt caaagtctgt
     6961 aacagctcga aggccacgca caatggctcg tgcgccaacc gttctacaat aatcgacaag
     7021 aagtccttcg aagctggcca cttccacgcc tggcatggat gcgagaactt cgcgtagaat
     7081 cgccatcctc tcttccactg tgaaaaatga attcttggat gaattgatgg caacgcttac
     7141 gatcacccgg tcaaagagct gcgaggctct ctgaaccagg tccaggtgtc cgtttgtaaa
     7201 aggatcaaac gatccggggt agactgctgt tttcaccgct atgtttcccg attatctttt
     7261 cgcgcccctt gcaatcagag tgtcttctac tttcttttca tctgcttcca tagtgaaagc
     7321 catcagaagc catccgatcg cgcacggaat ccagcaataa atggcgagat tgtatgcaag
     7381 agagtcgctt ccgaccagcc ttagaatttc accgaccaca aaaggtccaa gtcctttgcc
     7441 cagatcatct gcgaagttga acactccaaa gatcgatccg cgattttctg gaaggtttgt
     7501 atttaccagg atactgcgaa tgttcgggcc tgcctgagga atcacaattc cgccaagcgc
     7561 cgccgcgatc atagggtaga gcatagaatt tccctggaag ttgagaatgt agatcatggg
     7621 aacaattcca agtagaacgc aagcagcaca gaatactggc agcgctgctt tcttccaatt
     7681 gtaaactgcg cgaccaacaa accctccaat gaatccacca aagatcagaa cgcctccaaa
     7741 cacagtagtc agaagaacgc cgtcagatgg tttgaaccct tttgattttt catagtagtc
     7801 tacgagaaaa aagaagatga ggccccacgg aacgcaccct ggaattccct gaagaatggc
     7861 caggatgttt gtcttgctgc ttgcaattcg tttgaaatcg gagagtccaa gagaatgttt
     7921 tggttcttct cctgttgttt catgtacgtt ttctgctccg ccgcgttttg gagtttcgcc
     7981 gatggcaaga tacaggaata aaagagggaa gcatggtccg gccataatcg cgaaacttgc
     8041 gcgccatcct tctaaaccaa atacagtcgc agtcgcgagc attcctccta gaatctgtcc
     8101 gactccaatt ccaagaccca tcgcaagagc cagccagcca gaggcgctcg atctgttttc
     8161 aggtttgaaa tgatccccga gcaaagagaa aataatgggg aagattccac ccagaccaat
     8221 gccggttaac gttctaagaa cgatgtacgt cgtgtagttg ggcgcgaagg ccgtgaagaa
     8281 gcacgcaagc tcacctgcga acacagtgcc gaacaataga agcttgcgat tccagatgtc
     8341 tgtgaaggct ccaacgagaa tagaagcaat cccgccg
//
LOCUS       DAOQJL010000107         8344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4616_length_8344_cov_3.626493, whole genome shotgun sequence.
ACCESSION   DAOQJL010000107 DAOQJL010000000
VERSION     DAOQJL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_8344_cov_3.626493"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLG78_06545"
     CDS             complement(<1..680)
                     /locus_tag="PLG78_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22805.1"
                     /translation="MLAVKTVHWGRWIRRGIILAAMAAATGLHAVPDDTQARLEKLRE
                     NPLFQQLLDQSRPGLTDTQKKLLLKAIDSLSEQEIDDSLQELHLSTDGSATTRKDRLK
                     IAVGLMSAPELPKTKQGGAIGIENAAEGEFMRGENDGEGLMVLRGRIRLRLPSGIVLA
                     DTVIVDTKRQELFAEGNLVYYKTGEKDGKTGEIHAERLLYDQKLGTGILYNADGYMAP
                     VYFMGKSI"
     gene            734..2482
                     /locus_tag="PLG78_06550"
     CDS             734..2482
                     /locus_tag="PLG78_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE22806.1"
                     /translation="MLILDFYVDEPACFGVPPYLSPYARYAAGALVCAGIPQEKIEYL
                     TVDDWRAQGKELKNDPELVILIGGATVPGKYLGGKIGTVTEMQEFLEFRKKRQKGGVT
                     LMGGPVRFASRDIRESFEDLGGTLIRGDVEVYAERIARDPRSMREAADSFTHTGERWN
                     YEMVNRWAPAGAFLINLHPNFPWLILELETYRGCTRDVFCSFCTEALYGKPVFRALPP
                     IVEEVSELYKMGARHFRIGRQADLMTYLPDMNDFKNSFPRPVPASLEALYSGIRNAAP
                     DLKMLHLDNINPGLIATFEKESREIAHIICKYNTAGDTAAMGMESADIAVIEKNDLKS
                     TEQETYRAIEIINEAGARREGGVPKLLPGINLLHGLPGESEDTFKKNYEFLKRVIDNG
                     LLLRRINIRQTVTYEHTKLDQILKERTARKKDGIQNRFMYYRDRIRREIDHPMLLLNF
                     PPGTILRDVILESQNQGWYFGRPLGSYPVTVKIPLDDRAMNAKPGTPCDVIITGAEER
                     SLTALSYPVHLSMLGRKALETIPGAGRQRALRLQSERPLNFQDFSAIFEGRPFGKESD
                     YVFGVTGSEMSSSRPS"
     gene            complement(2445..2939)
                     /locus_tag="PLG78_06555"
     CDS             complement(2445..2939)
                     /locus_tag="PLG78_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNE22807.1"
                     /translation="MKKILTLLVLVLAVPVFAKPFNDKANKFKVSYPDGWKADTKAEH
                     FKVLFKSPDETANIGVAVVSVDKPVGAVDFLAEVEKQMGVENIMGQDERQLEDDANTQ
                     VGADDGAMGVFMRGKGESQLRQIMFIFTKGVKIYTVIITFNESDKETYRQLTKDVMNS
                     FQIQ"
     gene            complement(3008..3904)
                     /locus_tag="PLG78_06560"
     CDS             complement(3008..3904)
                     /locus_tag="PLG78_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE22808.1"
                     /translation="MAAKKSTRPAGKASSGPRVLPVPEQRKHVRKPGEIICYSWNVNG
                     IRAISRKGFREWFEDRNPDVLCVQETKAELEQVAKEKESQFLVDLEGYDSSWFSAEKK
                     GYSGVATFTRKSLEASSSKGFKKDGPDHFNNEGRVLVTTFGDFEIWNAYYPNGGRGPD
                     RVEYKLRFYDHCLDLWQKARKTKKIILTGDFNTAHKEIDLARPEENSTNTGFLPEERA
                     FLDKMIDAGYVDVFRHFEKGPNWFTYWDQVTMARNRNVGWRIDYFFVTEETMPLVTDA
                     WIEMDVPGSDHCPIGISLKSQS"
     gene            complement(3907..4974)
                     /locus_tag="PLG78_06565"
     CDS             complement(3907..4974)
                     /locus_tag="PLG78_06565"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22809.1"
                     /translation="MNTEIDFLREMMKRLDLFFLEADDQGKIRAISGGLIRLLGYPAG
                     HEITLQSLFDETTVAELFGRKLSAWQNGLRFYRAKVKNAAGEQIFFDVSGYMEIAPGG
                     RRFRVMLRELGPLDLALESGAFLENVHAYSIAQRYISRDLITRARRAANMGNKQLLNE
                     TRVLTFLFADLVSFTAYSETKTPDEIVQMLNLSIGAASSNIIHYNGVVDKIMGDSVFA
                     IFEQPLNAVLAAVEMQKQFQILNLFNVKTNQPEIQVRVGIHTGECIQASLGTEDHMEW
                     TAIGDAVNVASRLEKAAAPGTILASDHTVELIREKVTTVKELDVAMKGKTLPMKAYYI
                     TRVTFDGPRGPITLGVDDDVF"
     gene            complement(5068..5913)
                     /locus_tag="PLG78_06570"
     CDS             complement(5068..5913)
                     /locus_tag="PLG78_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE22810.1"
                     /translation="MIINHNLSAIDSHRTLKFQHWQVDRNMEKLSSGMRINRAGDDAS
                     GLAVSEKMRAQVSGLRQAERNTEDGMSLIQTSEGYLQEVSDIVQRIRVLAVQSSNGIY
                     SAGDRQMIQVEVSQLVDEVDRIASQAEFNKMSLLKGDFARGSRVSSMWFHIGPNQHQR
                     ERVYIQTMTAQALKLKRTDGTLLTLSTAEFSNDAIGVLDDALMRINKQRADMGAYYNR
                     LEHASKGLMVAYENTQASESRIRDTDMAEESVSFTKNQILVQSGTAMLAQANVRPQSV
                     LQLLR"
     gene            complement(6040..8304)
                     /locus_tag="PLG78_06575"
     CDS             complement(6040..8304)
                     /locus_tag="PLG78_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE22811.1"
                     /translation="MRQTLFALSLLVFTSLFLYVRASANVLPVVSDHAPEQEFSALRA
                     LVHLRNIARSPRPVGSSENERVRHYLVETLRHEGVDVRILKGSAVRTLIPRVHAAGNV
                     YNVLAVIPGKRKDSFLFLTHYDSVPTGPGAADNGASVVSALETIRALSKEKPEDTLQF
                     LFTDGEEAGLLGAASFVSRAENTSNVVAVFDFEARGISGESFLFQTGKKSSGLLQAFL
                     ENASGVAGSSLGPALYDVLPNDTDFSVFKRRNFVGLDFAFIGDVQAYHTSLDRPENLD
                     LRTLQHQGENMLTLARTREILDSNIETYFSPWPGIVLHYGSFVTRSFLILLLILAVLA
                     SRVIALRRSAAAFLYSIACIAVAALIGFGSWPTLSWLNGSTASLLGQPHNYFAYQLYF
                     ISLGVSAVFLIRFFALRRYSTEEVSVAFLWTAAACSVAAVILAPGAAYIFLLPAIACA
                     VCILLSRRFPGGFFSPAFALIFALSIVLIAWDPISGLFLALTLRAGFLAVILVCFYLL
                     LALHFVDLMLSTRHTLILSTVMFALLAAAVRVLSESPGPVLNSLAYGSEVETGKAFYF
                     SCAEKNDSFTSRFFGQAVRTGVIPAFPVMKQCLSGDLFQLATAPVFSNEPPVLQYSSE
                     RRGKKRWIHAKITSPRHASGLIVSVHTNPEIEKMVLFGRELPMQLEYGEGKKGLQFVK
                     RILRLDEWHTIMFAGLDEKGMELELEVNGNSTVEIRVTDRSRTILSQRPPGMIEAPEF
                     PFSDGVFITRTFKL"
BASE COUNT         2139 a         2110 c         2054 g         2041 t
ORIGIN      
        1 tggatgctct tccccataaa gtagacggga gccatgtagc cgtccgcatt gtatagaatt
       61 ccagttccca gcttttgatc gtagagtaaa cgctccgcgt gaatctctcc tgtctttccg
      121 tctttctctc ctgtcttgta atagaccagg tttccttccg caaaaagttc ctgacgtttc
      181 gtatctacaa tgacagtgtc cgcaagaacg atcccggaag gaagtctcag acgaatcctg
      241 ccgcgaagta ccatcaaacc ttcgccgtca ttttcgccgc gcatgaattc gccttctgct
      301 gcattctcaa tgccgatggc gccgccctgt tttgtttttg gaagctcagg agcgctcatc
      361 aaaccgactg caatcttcag tctatccttt ctggtagtag cacttccatc tgtggaaaga
      421 tgaagttcct ggagagaatc gtcaatttcc tgctcactga gagagtcaat tgccttcaaa
      481 agtagtttct tttgagtatc agtgagaccc ggacgacttt gatcgagcaa ttgttggaag
      541 agtggatttt cgcgaagctt ttcgaggcga gcctgggtgt cgtccggaac cgcgtggagc
      601 ccggttgccg ccgccattgc cgcaagaatt attccccggc gaatccatct gccccagtga
      661 accgttttca cagcgagcat acactatttt tgatgcgcgc gctaacgcaa agcatgaatt
      721 ggagcctcaa agcatgttga ttctagattt ctacgtagac gagccggcct gtttcggtgt
      781 gccaccgtat ctttcgccgt atgcaagata cgctgccgga gcgctcgtct gtgctggaat
      841 tccgcaggaa aagatagagt acctcactgt ggatgattgg cgcgctcaag gaaaggagct
      901 gaagaacgat cctgaactcg tcattttgat cggcggagcc acggtcccag gaaaatatct
      961 gggcggaaag attggcacag tcacagaaat gcaggagttt ctggagtttc gcaagaagag
     1021 gcaaaaaggc ggagtcactc tgatgggtgg accagttcgt tttgcttcca gggacattcg
     1081 agagagcttc gaagacctgg gtggtactct cattcgcggc gacgttgaag tgtatgcaga
     1141 aagaatcgcg cgcgatccca gatccatgcg cgaagctgcc gattctttca cgcacacagg
     1201 agaaagatgg aactatgaga tggtcaatcg ctgggctccg gccggagcgt tcctgatcaa
     1261 tttgcatcct aattttccct ggctcattct tgaactggaa acatacaggg gttgcacacg
     1321 cgatgtattc tgttccttct gcacggaagc actttacgga aaaccggtgt ttcgcgctct
     1381 tcctcccatc gtagaagagg taagcgagct ctacaaaatg ggggcgcgcc acttcagaat
     1441 cggacgtcag gcggacttga tgacctattt gcctgacatg aacgatttca aaaacagctt
     1501 ccccaggccc gtgcctgcaa gcctcgaagc gctctacagc ggaattagaa atgcggcgcc
     1561 cgatctgaaa atgcttcatc tggacaacat taacccgggg ctcatcgcga cttttgaaaa
     1621 ggaatccaga gaaatcgctc acatcatttg taagtacaac actgccggtg acacggccgc
     1681 gatgggaatg gaatccgcgg atatcgcagt cattgaaaaa aacgatctca aaagcacgga
     1741 acaggaaacc taccgcgcga ttgaaatcat caatgaggct ggagccagac gcgaaggtgg
     1801 cgttcctaaa cttttacctg gaatcaatct attgcacggc ctgcccggtg aaagcgaaga
     1861 cactttcaag aagaactatg aattcctgaa acgtgtcata gacaatgggc ttctgcttcg
     1921 aagaatcaat atcagacaga cagttacgta tgaacataca aagctcgatc aaatcctgaa
     1981 ggaaagaaca gcaagaaaga aagatggaat ccagaatcga ttcatgtact atcgcgatcg
     2041 gattagacgc gaaatagatc atcctatgct tctcttgaat tttcccccgg gaactatcct
     2101 gagagatgtg atccttgagt cacagaatca gggctggtat tttggtcgtc cgcttggctc
     2161 ttatcctgta acagtaaaaa tccctctcga tgaccgcgcg atgaatgcga aaccggggac
     2221 tccgtgcgat gtaatcatca ctggagcaga ggaaagaagc ctgactgcgc tttcttatcc
     2281 ggtgcatctt tccatgctcg gtcgcaaggc tttagaaacg attccaggtg ccggaagaca
     2341 aagagcgctt agactacagt cagagcgccc tctcaatttt caggatttct ccgccatctt
     2401 cgaaggaaga cccttcggaa aggagagtga ctacgtgttc ggagttactg gatctgaaat
     2461 gagttcatca cgtccttcgt gagttgacgg tacgtttctt tgtcagattc attgaacgta
     2521 atgattacag tataaatctt cacgcctttt gtgaagatga acatgatctg gcgaagctga
     2581 gactcacctt tgccgcgcat gaagactccc atcgcaccat cgtctgcgcc cacctgggtg
     2641 ttcgcatcat cttccagctg ccgttcatcc tgccccatga tgttttccac acccatttgc
     2701 ttttccactt ctgctagaaa atcaacagcg cctactggct tatctacaga aaccacagca
     2761 acaccaatgt tcgcagtctc atccgggctt ttaaacaata ctttgaaatg ctctgctttc
     2821 gtgtctgctt tccatccgtc gggataactc actttgaact tgtttgcttt gtcgttgaaa
     2881 ggtttcgcga agacaggaac tgcgagcacg agaactagaa gagtgaggat ttttttcatt
     2941 gagttaaccc ggaaaggaat ttggatgata gacgcgcgca ggcccggaac gttcaacctt
     3001 tttcgcatca gctctgcgat ttcaatgaga tcccaatggg gcaatgatcg cttcctggaa
     3061 cgtccatttc aatccaggca tctgtaacca gtggcatggt ttcctcggtc acgaagaaat
     3121 agtcaattcg ccagcccaca tttcgattcc tggccatggt cacctgatcc cagtatgtaa
     3181 accagttcgg tcccttctca aaatggcgaa atacgtccac gtaacccgca tcgatcatct
     3241 tgtcgaggaa cgccctttcc tcaggcaaaa agccagtgtt cgtgctattt tcttccggac
     3301 gcgcaagatc gatttccttg tgagctgtat tgaaatctcc ggtgagtatg atcttctttg
     3361 ttttgcgagc tttctgccat aggtccagac agtggtcata gaaacggagc ttgtattcta
     3421 ctctgtccgg ccctctccca ccatttggat agtacgcatt ccatatttca aaatcaccga
     3481 atgtggtaac gagaacccgg ccctcattat tgaaatgatc cggtccatct ttcttgaatc
     3541 ctttggacga tgacgcttcc agtgacttgc gagtgaacgt ggccactccg ctgtatcctt
     3601 ttttctccgc agaaaaccag gacgagtcat atccttctag atccacgagg aactgtgatt
     3661 ccttctcttt ggcaacctgt tctagttctg cttttgtttc ttgcacgcac agtacgtcag
     3721 ggttgcgatc ttcgaaccat tcgcgaaaac cttttctgga aatggcgcga attccgttta
     3781 cattccacga atagcagatg atctcacctg gctttcgcac gtgttttctt tgctccggaa
     3841 cgggaaggac tctcggacca gagcttgctt tgcctgcagg gcgagtggat ttcttcgcag
     3901 ccataattag aaaacgtcgt cgtctacgcc cagagtgatc ggaccgcgcg ggccgtcaaa
     3961 tgtgacgcgt gtgatgtagt acgccttcat ggggagtgtt ttccctttca tggcaacatc
     4021 cagttctttc acggttgtga ctttttcgcg aatgagttcc actgtatgat cggacgcgag
     4081 aatggttcca ggcgcggccg ccttctcaag gcgagaagct acgttgactg catccccgat
     4141 tgcagtccat tccatgtgat cttctgtgcc cagacttgcc tgaatgcatt cgccagtatg
     4201 aattccaacg cgcacctgaa tctcaggctg gtttgtcttt acgttgaaca aattcagaat
     4261 ttgaaactgc ttttgcattt ccacagcggc aagaactgca ttgagtggct gctcaaaaat
     4321 cgcaaacaca gaatccccca tgatcttatc cacgacacca ttgtagtgaa tgatatttga
     4381 ggaagccgca ccgatcgata gattgagcat ctggacaatc tcatccggtg tctttgtttc
     4441 agaataggcg gtaaagctca cgaggtccgc gaagagaaaa gtaagaactc gcgtttcgtt
     4501 gagcagctgt ttgttgccca tgttggccgc gcgtctggcc cgagtgatca aatcgcgcga
     4561 gatgtatctc tgcgcaatgc tgtatgcatg cacgttctca agaaaagcac cgctttcgag
     4621 cgcaagatcc agaggtccaa gttcgcgaag catgacgcga aagcgtctgc ctccaggtgc
     4681 aatctccata taaccggata catcgaagaa gatctgctca cccgctgcgt tcttcacctt
     4741 cgcgcgataa aacctgagac cattttgcca ggcagaaagt ttgcgtccga acaactccgc
     4801 aactgtggtt tcatcaaaaa gactctgaag agtgatctcg tgtccggccg gatagcccag
     4861 gagtcgaatg agtccgcctg aaatggcgcg gattttgccc tgatcgtcgg cctcaaggaa
     4921 gaagaggtcg agtcgtttca tcatctcacg aagaaaatcg atctcagtat tcacgccgcg
     4981 agtaaagtcg ggggtcgggt tgtcagcgca agtattaaat ggaggaggaa ggaaaaccgg
     5041 ccctgaaacc agggccggtc atttttttta gcggagcagc tgcagtacac tctgaggacg
     5101 aacgttcgct tgcgcgagca ttgccgttcc agactgtaca aggatctggt tctttgtgaa
     5161 agacacgctt tcttctgcca tgtccgtatc acggattctg gattcagatg cctgtgtatt
     5221 ttcgtaagca accatcaggc ctttggaagc atgctccagc ctgttgtagt aggcgcccat
     5281 gtctgcgcgc tgtttgttga ttctcatcag cgcgtcgtcg aggacaccaa ttgcgtcatt
     5341 cgagaactca gcggttgaga gagtgagcaa cgtgccatcg gtacgcttca gcttgagagc
     5401 ctgcgcagtc atcgtctgga tgtatacgcg ttctctctgg tgctgatttg gaccaatgtg
     5461 gaaccacatt gaagagacgc gagagcctct tgcaaagtcg cctttcagga gagacatttt
     5521 attgaactct gcctgtgaag cgattctgtc cacttcgtca actagttggg aaacttccac
     5581 ctggatcatc tgacggtcac ccgcagagta gataccgttg gaagactgaa cagccagcac
     5641 ccggattctc tgaacgatat ctgaaacttc ttgcagatac ccttccgatg tctggatcaa
     5701 gctcataccg tcttcagtgt tccgttctgc ctgacgcagg ccagagacct gtgcacgcat
     5761 tttttcggaa accgcaagac ccgaggcatc gtcgccggca cggttgatac gcatgccact
     5821 cgacagtttc tccatgttac gatctacttg ccagtgctga aacttcagcg tacggtgcga
     5881 gtcgatcgca gataggttgt ggttgataat cataaagttt cctccgtgaa acttctagaa
     5941 ctcggtcacg gatgagcatc cttgctcatt ttttccgttt ccacattcag cttcgggaaa
     6001 agcttgagta aaaattaggt cgaacgacga cttttttggt tagagcttga atgttcttgt
     6061 gataaacacg ccgtctgaaa aaggaaactc gggcgcttct atcattccgg gtggtctttg
     6121 agagagaatg gttcgcgagc gatctgttac gcgaatttcc acggtcgaat ttccgttcac
     6181 ttctagctca agttccatgc ccttttcgtc gaggccggcg aacatgattg tatgccattc
     6241 atccaggcga agaatgcgtt tcacgaactg aagacctttc tttccctccc cgtattcaag
     6301 ctgcatcgga agttcgcgcc cgaacaaaac catcttctct atttcagggt tcgtatgtac
     6361 ggatacaatg agcccggatg catgccgagg cgaagtgatc ttcgcgtgaa tccatctctt
     6421 cttccctctt ctctcgctcg agtactggag cacgggcggt tcattggaaa aaacaggagc
     6481 agtggcaagc tgaaatagat ctccggacag gcattgcttc attacaggaa acgcaggaat
     6541 cacacccgtg cgaactgcct ggccaaagaa gcgagaagta aacgaatcgt tcttctcagc
     6601 acaggagaag taaaacgcct tgcccgtttc tacttcgctt ccgtatgcaa gtgaattcaa
     6661 aacaggacca ggagactcag acagcacgcg aacagccgcg gcaagaaggg caaacattac
     6721 ggttgataga atcaacgtat gccgcgtgga caacatcaga tccacaaaat gaagggctag
     6781 aagcaagtag aaacaaacga gtattacagc aagaaaaccc gcgcgcagag tgagagccag
     6841 gaacagtccg gaaatcggat cccaggcaat caatacgatc gacagtgcga aaatcagcgc
     6901 aaatgcaggt gaaaaaaacc cgccaggaaa tcgcctggaa agtaagatgc acacagcaca
     6961 tgcaattgcc ggcaagagga agatgtatgc tgcgccaggc gcgaggatga ctgctgcaac
     7021 agaacaggcc gccgcagtcc atagaaatgc aacagaaact tcctctgtgc tgtatcgcct
     7081 gagagcaaag aagcgaatca agaaaacagc agatactccc agagaaatga agtagagctg
     7141 gtacgcgaaa tagttatgtg gctgcccgag aaggcttgca gtgctgccgt tgagccagct
     7201 caaggttggc catgaaccaa atccaatcaa agccgcaacc gcgatgcatg caatagaata
     7261 cagaaatgct gcggcagatc gccttaaggc aatgacacgc gaagcaagca cagccaggat
     7321 caaaagcaga atcaaaaacg agcgcgtaac aaaactgcca tagtgaagca caattccagg
     7381 ccacggactg aagtatgttt caatgttaga atccagaatc tctcgcgtgc gtgcaagagt
     7441 gagcatgttt tctccctggt gctgcagagt gcgaagatcc agattttctg gacgatccag
     7501 ggaagtgtgg tatgcctgaa catctccgat gaaagcaaaa tcgagtccta cgaaattgcg
     7561 tctcttgaat acgctgaaat cagtatcgtt gggcagtacg tcgtagagag ccggcccgag
     7621 ggaactgcct gccactccag atgcattctc cagaaaagcc tgaagcaaac cactgctctt
     7681 cttacctgtc tgaaatagaa acgattctcc agaaattccc ctggcttcga aatcaaagac
     7741 cgctaccaca ttcgacgtgt tctccgctcg agatacaaac gaggccgctc ccagaagtcc
     7801 tgcttcttct ccgtctgtaa aaagaaactg aagagtgtcc tctggctttt ctttcgaaag
     7861 agcgcgaatg gtttcgagag cggacacaac acttgccccg ttgtctgctg ctccgggtcc
     7921 cgttggaacc gaatcatagt gagtcagaaa taagaacgag tcctttctct ttcccgggat
     7981 cacagccaga acgttgtata catttccagc tgcatgcacg cgagggatga gtgtgcgaac
     8041 tgcagaaccc tttagaatgc gaacatccac accctcatgg cgaagagtct cgaccaggta
     8101 gtgcctcact ctctcatttt cactggaacc aactggtctt ggagatcggg cgatgtttcg
     8161 cagatgcacg agtgcgcgaa gagccgaaaa ctcttgctcg ggtgcatgat cggaaacaac
     8221 aggcagcacg ttggcgcttg cacgcacgta gagaaaaaga gaagtaaaga caagaagaga
     8281 aagagcgaac agggtttgcc gcattctcac tgaatgcgga atcgttccag agggtcaaga
     8341 atgt
//
LOCUS       DAOQJL010000108         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4658_length_8298_cov_3.778843, whole genome shotgun sequence.
ACCESSION   DAOQJL010000108 DAOQJL010000000
VERSION     DAOQJL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_8298_cov_3.778843"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..689
                     /locus_tag="PLG78_06580"
     CDS             24..689
                     /locus_tag="PLG78_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22812.1"
                     /translation="MRKKENPLRIGVCSLDELEEKVKAFRIMNQTALKKRYVLSREDI
                     ITSAGETIVQKRSEVDISRAKLMRRHFQGDKILKTFQPDEGIVIISDMSTAEGIPLSM
                     DLVTQVMNLGGGAYEAFIDRVDFFSDFLNLLKKALFPRLVIIGYLPGDRLDVEQMNFL
                     RARKIDQYMRVIELTHTAFKPKPYFTKVKHIVIDISEPKTWGRFIIELVREYTKPYLF
                     EEF"
     gene            689..1408
                     /locus_tag="PLG78_06585"
     CDS             689..1408
                     /locus_tag="PLG78_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE22813.1"
                     /translation="MFLNLLFFLLLSPDRAREMYRTSLKLDDVRAVELLARAYEEDKT
                     NQDVTYRLGYLYHKMNRTREAVDYYSKTVRLNSCHERALNNLGTIAYQEKKKQEARSY
                     YEKAAKCGKDAVTPLYNLANLESEEGNEEAARSLYERVVRKDPNHARSRHNLGLLYFR
                     RYNQTGSDVDLEAAEKQLKEAVRLSPSDPLTLFNFALVLEAGQKTDEAIRVLKKADSL
                     SSGKTIQPRIREALARLKGKG"
     gene            complement(1415..3862)
                     /locus_tag="PLG78_06590"
     CDS             complement(1415..3862)
                     /locus_tag="PLG78_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE22814.1"
                     /translation="MALDPAKALQQAKIPAGTVLFEAGKAATMLWMVHEGEVTLREPS
                     GRKLYNLGPSSVPGFSALMLQKPYAASYTASKDTVISAFPVNPAGNYNSLVLGKLNVG
                     IAAARSMVQEAILSRKTFVAASEFGGEIQKAIDNLSLAYYKLNPGAFQQAPGGGGQVL
                     DPVVPQTRVLLEEFQSSGGEIPDIITAGWLEQDHSSLLKKHYEMESEFDEEEFTFLRR
                     ILSLPADLQGNMFKADPALLQIISAKLATIVYAGIGELRQIEETILESTNAVLSGEYS
                     FIEKFFLVADVQGEEGRPNVIGVVKFLVTFAEKLKSRHESMFGKPIGSIAASLDNLRE
                     MVKTQKPEEKAQEATAAAVAVGVDIQALKKELAGSIGKILNFVQLPAEDGRKLLADLK
                     TLKSFNNPLDSGGDPRKVRRAINKTYWSIYEKTFFKMKETKILPLPVDWMLRFGFFDE
                     EMLDDEHIAQLTQCKDSTRGKPGYSVLSALDWLPYVGDKKEPPSLDEMGMGFFEKLKQ
                     EHKDKGWKKETDVPDEYAGYAIRVRYEILNFLEINVRLTSGSPATSFPILTRYQINTP
                     LDKAFVTADKLSECIDKILAIDYSLFHRETLFNDESRGILKEFIQLQVIPYFIIVPSI
                     GTKVMMWQDLASPNKRTSKGRIAIPVFATADLFSIMVDAMAAFRWELTKSIQGFEWNN
                     VAVPSITADYTDYVQFYKKNRELSPEIKEKLASEFKRFRSDRDRFANDYSNWLKGEAE
                     GNMKMNRVVRAIFYRHVPFTKGVRDKVATQPAFAELHNRFVNIRTKKLREFEMKYRKY
                     SEPLPDVLQKNLDFYRV"
     gene            complement(3909..4571)
                     /locus_tag="PLG78_06595"
     CDS             complement(3909..4571)
                     /locus_tag="PLG78_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNE22815.1"
                     /translation="MSLQNLERLKARLEQLGHPEVRVVAISKTHPAEKITELLRAGHR
                     DFGENRFAEARDKIPAVDTTGLAPEQMPIYHHIGPLQSGNARQISGLFQVVHGASSLS
                     GIQALNKACHGRSESLRYFIQLNLTGEQSKMGGMTIEELDSLGEPPAGPGFEFAGLMT
                     MGPSDSEPKKTREVFRSLRRIRDERFSGMELSMGMSQDYEIALEEGSTLLRIGSLIFG
                     SR"
     gene            complement(4552..5034)
                     /locus_tag="PLG78_06600"
     CDS             complement(4552..5034)
                     /locus_tag="PLG78_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22816.1"
                     /translation="MQISGKYLLSSAETLLNRRSNRIASQGTGPDTSRELQPALMHSR
                     LMQIQGHLAHVQHDYSREQARLASVDAVEGRENLQFDGAPLFPEKDTGDLRDKITDRL
                     DVLGKQLKSLQVEMENMVALGFKPEEYKIDPKLAHDTSMRPIDPDRVARLTRSVPSES
                     "
     gene            5206..6174
                     /gene="sppA"
                     /locus_tag="PLG78_06605"
     CDS             5206..6174
                     /gene="sppA"
                     /locus_tag="PLG78_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNE22817.1"
                     /translation="MDRSRAAVALALGLATVSLLLGLLQIGMARKSQDFTAPAGALFN
                     AGKAGIALIEVRGVIEDGSGGPGGQGADRIVERIRSAQEDPAVRGMLLSINSPGGTTG
                     ATKKIYDKLIEFRKEKPIVCYVTDIAASGGYYLCSAANKIYAYRGSIIGSIGVIMIHP
                     DAEELLRKVGLTVSVIKAGRFKDMSYPFRTMTEEERLMNQKLVDDAYEQFVRDVAEGR
                     RQPETNVRKWAEGKIYSGTSAKAEQIIDEFGGETEALLSMKTEFIKTKDDLPLLRPRK
                     EFFEEFLTGLSGRSYDLSSGRVYYLYPDSTTVARMILGPDLVKIAK"
     gene            6171..6749
                     /locus_tag="PLG78_06610"
     CDS             6171..6749
                     /locus_tag="PLG78_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22818.1"
                     /translation="MNWLSKFVELAAAILQKPEDSVRFANNPGPFSSIIPAFSAFSLA
                     AAGYLLRDYYEGIFLFTISFHASVLTLVYVLFASLTSAVLDARVQTQSGRSPRAHQAR
                     RVLLQSLLPLIFAFPAATIAHITPVPGFVFVPLLLLLYAWAFITALRSLQYLYELSIR
                     DIARQLVISSVITLAFPAALVVLLFVRLAAFL"
     gene            6758..7492
                     /gene="surE"
                     /locus_tag="PLG78_06615"
     CDS             6758..7492
                     /gene="surE"
                     /locus_tag="PLG78_06615"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE22819.1"
                     /translation="MRILLTNDDGLFTNGMLHLEEALRDGAEVWCIAPDRERSATSQA
                     LSLRETLLLKKISDRRYTVNGYPTDCVNIALFSGEFPRFDLVISGINHGVNLGDDIHY
                     SGTVGAARHATLHGHPAIAMSCPIRGHDGDFRRPAAWLAQWIKSNLKALQPGKLWNIN
                     YPEESAGANGFPEVVITRQGRRIYQDRYELLSEGEGSRIYKLLETTMGRKMEPGTDFD
                     AFESGRISLTPITTDTTDQERLYGAL"
     gene            7489..8136
                     /locus_tag="PLG78_06620"
     CDS             7489..8136
                     /locus_tag="PLG78_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22820.1"
                     /translation="MKSEKNEKQVLERILTIKQMIQEGRGGPLELHELGICYYLIENF
                     RSASKFLAELAGKYPDYVEIASVQSLRCYCLVQEGELLEAELLLKERIPRDPSNTTLL
                     GLLAHVQEKTHRIQEAIATHKRIIGIDPGNINSRNSIGCLLAEHGTPADYPEAMEHLR
                     IAVSNKPEHPAYLDSLGILLARQGDRDRARRAFVKALSRSPANTVILDHLKDMIG"
     gene            complement(8160..>8298)
                     /locus_tag="PLG78_06625"
     CDS             complement(8160..>8298)
                     /locus_tag="PLG78_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNE22821.1"
                     /translation="HPLPRVAELSTDVDDMPTAAYFRQAGYGVVIRMALLMLCLGQPI
                     P"
BASE COUNT         1921 a         2049 c         2155 g         2173 t
ORIGIN      
        1 aggatgaggc cagtaaagag gaaatccgaa aaaaagagaa tcctctgcgc attggcgttt
       61 gcagcctgga cgaactcgaa gagaaggtga aagccttccg aatcatgaat cagacggcac
      121 tcaagaaacg gtatgttctc tctcgagagg acatcatcac aagtgccggt gaaacgattg
      181 ttcaaaagcg gtcggaggta gatatttccc gggcaaagct gatgcgccgc cattttcagg
      241 gagacaagat cctcaaaaca ttccaaccgg atgaaggcat tgtaatcatc agtgacatgt
      301 ccacagcaga gggcattcct ctttccatgg atcttgtgac gcaggtgatg aatctgggcg
      361 gaggtgccta cgaggcattc attgaccgcg ttgatttctt ttctgatttt ctgaatttgc
      421 tcaagaaagc attgtttcca agacttgtca tcattgggta tctaccagga gacaggctgg
      481 atgtggaaca gatgaacttt ctgcgtgcac gcaagattga tcaatacatg cgcgtcattg
      541 aactcactca caccgcgttt aagccaaagc cttactttac gaaagtgaag cacatcgtga
      601 tcgatatctc agagccaaag acatggggac gattcatcat tgagctggtt cgcgaataca
      661 cgaagcccta tttgtttgaa gagttttagt gttcctcaat ctacttttct ttcttcttct
      721 gtccccggat cgcgcgcgcg aaatgtatcg cacatctctg aagctggatg atgtgcgcgc
      781 tgttgaactc ctggcccggg cgtacgaaga agataagaca aatcaagatg taacctaccg
      841 gctcggctat ctctaccata agatgaaccg tacccgtgaa gccgtggact actattcaaa
      901 aactgtgcgg cttaattcct gccacgaaag agcgctcaac aatctgggta ccattgccta
      961 tcaggagaag aaaaagcaag aagcacgcag ctattatgag aaagcagcga aatgcggcaa
     1021 ggatgcagtt acaccactct acaatcttgc aaatctggaa agtgaggaag gcaacgaaga
     1081 ggccgcccgg tctctgtatg agagagtggt ccgcaaggac ccgaaccatg cccgaagtcg
     1141 gcacaatctg ggacttttgt attttagaag atacaaccag actggatcag acgtggatct
     1201 ggaggcggcc gagaagcaac tgaaagaggc ggtgcggcta tctccgtccg accctctcac
     1261 tttatttaat tttgccctgg ttctagaagc cgggcaaaag accgatgaag cgatccgggt
     1321 gttgaagaag gcggattctt tgtcttccgg caagaccatc cagccgcgca tccgcgaagc
     1381 cctggcgcgg ctcaaaggga agggctagca aaacttagac gcggtagaaa tcgaggttct
     1441 tctgcagcac gtctggcaga ggctctgaat acttgcgata cttcatttcg aattcgcgca
     1501 acttcttggt gcggatattt acaaaacgat tgtgaagttc tgcgaatgca ggctgcgtgg
     1561 ccactttgtc gcgcacacct tttgtgaaag gaacgtgtct atagaagatg gctctcacca
     1621 cgcgattcat cttcatattg ccttccgctt cgcccttgag ccagttggaa tagtcatttg
     1681 cgaagcgatc gcggtcactt ctgaatcgtt tgaattctga tgccagtttc tctttgattt
     1741 ccggagatag ctctctgttc tttttgtaga attgaacgta gtccgtataa tccgcagtaa
     1801 tggaaggtac ggcgacgttg ttccattcga atccctgaat gctttttgtg agttcccagc
     1861 ggaaggcggc cattgcatct accatgatag aaaacaaatc ggcagtggcg aaaaccggaa
     1921 tggcgatgcg ccctttggac gttctcttgt tggggctcgc gagatcctgc cacatcatca
     1981 ccttggttcc aatggaagga acaatgatga aataaggaat gacttgaagc tggatgaatt
     2041 ctttgaggat gcctctggac tcgtcattga aaagggtctc ccggtgaaac agcgagtaat
     2101 caatggccag aattttgtct atgcattcgg aaagcttgtc tgcggtcaca aatgctttgt
     2161 ccaggggagt gttgatctgg tatctggtca gaatagggaa ggacgttgcg ggagaaccag
     2221 acgtcaggcg aacattgatt tccagaaagt tcaggatttc atatcgcacg cgaatcgcat
     2281 atcctgcgta ttcgtccggt acatccgttt cttttttcca gcctttgtct ttgtgttctt
     2341 gcttgagctt ctcgaagaaa cccattccca tttcgtccag ggacggaggt tctttcttgt
     2401 ctcccacgta aggcaaccag tcgagtgcgg atagtacgct gtatcctggt tttccccgcg
     2461 tggaatcttt gcactgagtg agctgtgcaa tgtgctcatc atcgagcatt tcttcatcaa
     2521 agaaaccaaa gcgaagcatc cagtctacgg gaagcggaag gatctttgtt tccttcatct
     2581 tgaagaacgt cttttcgtat atgctccagt aggtcttgtt gatggctcta cgtactttgc
     2641 gtggatctcc acccgaatcg agcgggttat tgaaggactt cagagttttt agatctgcga
     2701 gtagcttgcg cccgtcttct gccgggagct gaacgaagtt gagtatcttg ccaatgcttc
     2761 ctgcgagttc tttcttgagc gcctggatgt ccactcctac ggcaacggca gcggcagttg
     2821 cttcctgcgc tttctcttct ggtttctgtg tcttcaccat ttctctgaga ttgtccagag
     2881 aagcggcgat cgatccaatt ggttttccaa acatgctttc gtgtctggac ttcaactttt
     2941 ctgcgaacgt gaccaggaat ttgacaacgc cgattacgtt gggtcgccct tcttctccct
     3001 gaacatctgc aaccaggaag aacttttcaa taaaactgta ttcgccagat aacactgcgt
     3061 ttgtgctttc tagaatggtc tcttctatct ggcgaagttc tccgattcca gcatacacga
     3121 tggtggcaag cttcgcagag ataatttgca ggagggccgg gtctgctttg aacatgtttc
     3181 cctgcaggtc cgccggtaga ctcaggattc gccgaagaaa agtgaactct tcttcatcaa
     3241 actccgattc catttcataa tgtttcttaa gtaaactcga atgatcctgt tcgagccagc
     3301 ccgcggtgat gatgtcagga atctctccgc cgctggactg gaactcttcg agaagcaccc
     3361 tggtttgcgg cacgacagga tcgaggacct ggcctccgcc gcctggagcc tgttggaatg
     3421 cacccggatt taatttgtag tatgcgaggg aaagattgtc gattgccttc tgaatttctc
     3481 cgccaaattc ggaagccgcg acgaatgttt tgcgagatag aattgcttct tgcaccatgg
     3541 atcgagcagc tgcgattcca acgttgagtt tgcccaggac cagggagttg tagttgcccg
     3601 caggattgac cggaaaggcg ctgatgactg tatctttgga tgcggtgtag ctggccgcgt
     3661 aaggcttttg tagcatcagg gctgaaaatc cgggcacact gcttggcccg aggttgtaga
     3721 gctttcgtcc agatggttcg cggagtgtaa cttcgccttc atgaaccatc cagagcatgg
     3781 tggccgcttt gccggcttcg aacaagactg ttccggcagg gatctttgcc tgctgtagag
     3841 cttttgctgg gtccagggcc attgaattgt cttcctacta ttttgaagct cgtcgtgcca
     3901 ctcaaaaact aacgtgatcc aaaaatgaga ctgccgatcc gaagcagggt cgacccttcc
     3961 tcgagggcaa tctcatagtc ctgggacatt cccatggaca gttccattcc gctgaatcgc
     4021 tcatcgcgga tgcggcgcag ggatctaaat acttctctgg tcttttttgg ctcagagtcg
     4081 ctcggcccca tggtcatgag acctgcgaat tcaaatccag gtccagcggg cggttcgccc
     4141 agggagtcca gttcttcaat cgtcattccg cccatcttgc tctgttcgcc ggtcaggttt
     4201 agctgaatga agtatctaag gctttcgctt cttccatggc atgctttgtt taacgcctgg
     4261 attccggaga gcgaagaggc gccgtgcaca acctggaaga ggccactgat ctggcgagca
     4321 ttgccagatt gcagggggcc gatgtgatga tagatgggca tttgttcagg tgcaagacct
     4381 gttgtgtcca cggccgggat tttgtcgcgt gcctctgcga atctgttttc tccaaagtcg
     4441 cgatgccctg cgcgaagaag ctccgtaatt ttttcggcgg gatgagtctt gctgatggcg
     4501 accactcgaa cttctgggtg ccccagttgt tcgagcctcg ctttcagtct ttcaagattc
     4561 tgaagggaca ctgcgagtga gcctggccac tctgtctgga tcgatgggac gcatggacgt
     4621 gtcgtgtgcg agtttaggat cgatcttgta ttcttccggc ttgaatccga gcgccaccat
     4681 gttctccatt tccacttgca gagacttgag ttgttttccc agaacatcca gtctgtccgt
     4741 gatcttgtcg cgcagatcgc ctgtatcctt ttccgggaag agtggagcac catcaaactg
     4801 gagattctct cttccttcta ccgcgtccac ggaagcaaga cgagcctgtt cgcggctgta
     4861 atcatgctgc acgtgagcca ggtgtccctg aatctgcatc agcctggaat gcatgagtgc
     4921 tggctggagt tcccggctcg tgtccggtcc ggtcccctga gaggcgatcc ggtttgagcg
     4981 gcgattcagc agagtttctg ctgaactcag taggtatttg ccggagatct gcatgatgat
     5041 gttcctctaa taatattatg tcgcacaggc tgtttcttgc gccgagttcc gtctacttag
     5101 tttttcgatc agtggacccg gtctctgtag tggttttttc tctcgaaata tgtcctttta
     5161 tcgaattttt ctcgctcgga ctcagcttcc tgtgtctctg gacccatgga tcgttcacgc
     5221 gctgctgtgg ccctggcact cggattggcc actgtatctt tactcctggg gttgcttcaa
     5281 atcggcatgg cccgcaaatc gcaggacttc actgcgcccg caggtgcgct cttcaacgcg
     5341 ggtaaagcag ggattgcact catagaagtt cgcggtgtta tcgaggacgg ttcaggcgga
     5401 ccgggtggtc agggagcaga tcgcatcgta gagcgaattc gctctgcaca ggaagacccc
     5461 gctgttcgcg gaatgctact atccatcaac agtccaggtg gaaccacagg cgccacaaag
     5521 aagatctacg acaaactcat tgaatttaga aaagaaaagc caatcgtctg ctatgtgact
     5581 gacatagcgg ccagcggcgg ctattacctt tgctccgctg ccaacaagat ctatgcgtat
     5641 cgcggatcta tcatcggatc gattggcgtg atcatgattc acccggatgc agaagaactc
     5701 ctgcgcaaag tcggcctcac agtcagcgtc atcaaagcgg gcagattcaa agatatgtcc
     5761 tatccatttc gcacgatgac agaagaagaa agactcatga accagaaact cgtggacgat
     5821 gcatacgaac aatttgtgcg cgatgtagcc gagggtcgtc gtcaaccgga gaccaatgtg
     5881 cgaaagtggg cagagggaaa aatatattcc ggaacgtctg cgaaggcgga gcagatcatt
     5941 gatgaatttg gcggagaaac ggaagcactt ttgtctatga aaacggagtt tatcaaaaca
     6001 aaagacgatc ttcctttgct tcggccgaga aaagaattct tcgaagagtt tcttactggg
     6061 ctgtccggtc gttcttacga tttgagcagt ggccgtgtat actatctgta tcctgattca
     6121 acgactgtgg ccaggatgat tctggggccg gacctggtta agattgcaaa atgaattggt
     6181 tgagtaaatt tgtagagctt gcggcggcca tccttcagaa accggaggac tctgttcgtt
     6241 tcgcaaacaa tccaggaccg ttcagtagca tcatacctgc tttctctgct ttttcgctgg
     6301 cagccgccgg ttatttgctg cgtgactact acgaaggcat ttttcttttc acgatttcct
     6361 ttcacgcctc tgtcctcact ctggtctacg ttttgtttgc ctctctcacg tctgctgtcc
     6421 tcgatgcgcg cgtgcagacg cagtccggaa gatctccgcg agcccatcaa gccagacgag
     6481 tcttgcttca gtctcttctt cccttgatct tcgcatttcc ggcggctacc atcgcgcata
     6541 taacacctgt gcccggtttc gtatttgttc ctcttttgtt gttgttgtac gcctgggctt
     6601 tcatcactgc acttcgctct cttcagtatc tttatgagct ttccattcgt gacatagcaa
     6661 ggcagctcgt gatttcttcc gtcatcactc tggcgttccc ggccgccctg gtcgtccttc
     6721 ttttcgttcg cctggccgca ttcctctagt tcagtagatg agaattctcc ttacaaatga
     6781 tgatggtctc tttacaaacg ggatgctgca tctggaagag gcattgcgcg atggggcgga
     6841 agtgtggtgc atagcaccgg accgcgaacg aagcgctact tcccaggcac tcagcctgag
     6901 agaaactctt ctcttgaaaa agatctcaga taggagatac acagtaaacg gctatccaac
     6961 cgattgcgta aacattgcac ttttttcggg agagtttccg cgttttgatc tcgtgatctc
     7021 tggaatcaat cacggcgtaa acctcgggga tgacattcac tacagtggaa ccgtaggagc
     7081 cgcgcgtcat gcgaccttac atggtcatcc agccatcgct atgagttgtc cgatccgcgg
     7141 tcatgacgga gatttcagaa ggccggccgc atggcttgct cagtggatca aaagcaatct
     7201 caaagcactg caacctggaa agttgtggaa tatcaactat ccggaagaaa gtgccggtgc
     7261 caacggtttt ccggaagtgg tcatcactcg ccagggcaga cgcatctacc aggatcgata
     7321 cgagcttctg tctgaaggag aaggctcacg gatttataaa ttactcgaga ctacgatggg
     7381 tcgcaagatg gaacccggaa ctgactttga tgcatttgaa tcaggcagga tttctctgac
     7441 tcccatcaca acagacacta cggatcagga aaggctatac ggagcgctat gaaatcggaa
     7501 aagaacgaaa agcaagtgct ggagagaatc ctgaccatca agcagatgat tcaggaagga
     7561 aggggcgggc ccctcgagct ccacgaactg ggtatctgtt actatttaat agagaatttc
     7621 agatccgctt ctaagttcct ggcggagctt gcaggcaagt atccagacta cgttgagatt
     7681 gcttccgtcc agagtttgcg gtgctactgc ctggttcagg agggggagct actcgaggcg
     7741 gagcttttgc tcaaagagag gattccgcgc gatccctcca ataccactct tctgggtttg
     7801 cttgctcatg ttcaggagaa gacgcaccgc attcaggaag caatcgccac tcataagcga
     7861 atcattggaa tcgatcctgg aaatatcaac agtcgcaatt ccattggatg cttgcttgca
     7921 gaacatggca ctccggcgga ttaccccgag gcaatggagc accttcgcat cgcagtgagc
     7981 aacaaaccgg agcatcctgc ttatctggat tctctgggca tcttgcttgc tcgacaggga
     8041 gatcgtgaca gggcgcggcg tgcctttgta aaggcgctca gcagatctcc agcaaacact
     8101 gtgatcctcg atcatctcaa agacatgatt ggttgatttt ctttagtaag tttgacgatt
     8161 caaggaatgg gctgtccgag acagagcatg aggagtgcca tgcgaatcac aactccgtag
     8221 cctgcctgac gaaagtaagc cgctgttggc atatcgtcta cgtctgttga aagttctgcc
     8281 acgcgaggaa gtgggtgg
//
LOCUS       DAOQJL010000109         8273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4669_length_8273_cov_4.303237, whole genome shotgun sequence.
ACCESSION   DAOQJL010000109 DAOQJL010000000
VERSION     DAOQJL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_8273_cov_4.303237"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..939
                     /gene="ric"
                     /locus_tag="PLG78_06630"
     CDS             238..939
                     /gene="ric"
                     /locus_tag="PLG78_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNE22822.1"
                     /translation="MILSSDTLSKIALSAPHATRVFAKYRMDFCCGGGTSLSDACIGK
                     GLNSESVLAEIQAAAPLRRSVEADAPLGEVVDHVLSTHHEFTRNIMAEIEKFSVRVAA
                     VHGAQHPEMIVLRDLFADFKAEMELHLLKEERVLFPYIRLLENGAEKDAPVCFSDVGG
                     PIRMMLFEHENAGEDLRKMREVTSDYTLPQAACNTYRLLFHKLQELEEDVHLHMHLEN
                     NLLFPRAMELAANPG"
     gene            991..1986
                     /locus_tag="PLG78_06635"
     CDS             991..1986
                     /locus_tag="PLG78_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNE22823.1"
                     /translation="MLRFLTIACVALCSCQWLIRPGTDFEKSPPPPEPDYSLRESWAA
                     LPDRADTADSVPSEADWKDAQSTASADVFFVHPTLYFGGTWNADVADATVNRRVDETT
                     LRKQASVFNCCARIYAPRYRQATIAAFLDKKNGAPALDLAYSDVLRAFDYYMIHYNQG
                     RPVIIASHSQGTRHAYFLLKDRFDRKPALSRLVAAYLIGMAIPEHPYATIPICKSATE
                     VRCFITYRSATPGTELKRLPHDPEPPYACTNPVHWKSDDSLAEADLNPGGVSANFEKV
                     DPNVCSAQCQNGVLLISKPKESGYHNISGNFHVSDYSLFYVSIRKNVADRIAAFR"
     gene            1983..2729
                     /locus_tag="PLG78_06640"
     CDS             1983..2729
                     /locus_tag="PLG78_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNE22824.1"
                     /translation="MSVLVLVLVVLFCSIALLFPRKQARSSSRIAAVKRNSPVILRQL
                     VYRGGDWDANPGSLRNLARFASNCEAIKLDVRAGEPANGRDLPGSDFFVYATGHHGFD
                     LAGSDLTGFLSFVRAGGFVLVDDNRGMDAAFRRFVAQNFPEARLEPVRSGEEVFRKPY
                     NVGSMPKIMIHDGSPAVLYRLKEGQDLNLYYSFSSDLGDGWEDSLRHRVPEETRRLAL
                     EMGTNLIALAFAWSSAARERRGNFAARNSS"
     gene            complement(2767..2925)
                     /locus_tag="PLG78_06645"
     CDS             complement(2767..2925)
                     /locus_tag="PLG78_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HNE22825.1"
                     /translation="MDIVRIIAAIVLPPLGVFLQVGIGFHFWLNILLTIFGYVPGIIH
                     AIWVILKK"
     gene            complement(2936..3349)
                     /locus_tag="PLG78_06650"
     CDS             complement(2936..3349)
                     /locus_tag="PLG78_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22826.1"
                     /translation="MKPLLLTMALFFSSHALFAQEAILSKPKACLAVDENAGTPSGCK
                     TKFRAADKIHVFSNIETPGVYKLNAVFELQDDTRKNKPINIDIDTQADWNYVYFWMTP
                     NTHDDKYLGTWKITIYVHTEDGDTSEYSTTFQVVK"
     gene            complement(3394..4038)
                     /locus_tag="PLG78_06655"
     CDS             complement(3394..4038)
                     /locus_tag="PLG78_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE22827.1"
                     /translation="MDRRKFFREGVKEVAREVMKTPVGSAIDKQLLAFSNLLSPEHFT
                     PPAPVPPADAPPPRKLARPPGALKDPKKFNKACTKCGDCIVACPYGVLFQMGPASGPL
                     LDPNLNACRLCPDMPCIDSCETGALKKLRKKQNPRLGVAAVSESLCRNHESRKPRLAP
                     GQKSQTCKICVRECPVKGAISLGKDRLPDIAKSCTGCGQCVHVCPNHAIRVDVF"
     gene            complement(4025..4426)
                     /locus_tag="PLG78_06660"
     CDS             complement(4025..4426)
                     /locus_tag="PLG78_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNE22828.1"
                     /translation="MGFLDKVISAANDVAGKEGNQNLVGGVIDMLQKKGVGGIVQDLK
                     TKGLGDLAESWIGTGENKLISPEQIKNVLGSEQLQALAQKAGVSPENASKFLGQILPG
                     IVDKLTPQGQVPGDDPGKSPEASAGPSDGST"
     gene            4497..5495
                     /locus_tag="PLG78_06665"
     CDS             4497..5495
                     /locus_tag="PLG78_06665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE22829.1"
                     /translation="MNPEILRRYYMQVLEAPKKKRRAPKKSKDPFHPPLFEARPGHVR
                     LVPCNACGGMSFKTLFEKESSLKEVFQVVRCKCGLVQVNPQPDLQAVLPYYADSYFKK
                     RTDRGYDNYFSDEIKAEIRRVFTMNLEDIGFFAYEKKVFEKQRPGRALDAGCAAGYFV
                     EYMAERGWNAEGIELSAVAAKPGKARGLRITVGDFLTHSDLIPQSYDFISFWASIEHM
                     HEPARVLLRASELLQPGGRMVLSTCRYGLLARLAGRDWRFMNVPEHLYFFSVKRLTRL
                     ASELGFKVVGKVTYGSGLTTRKDASPFYRFAKRWLDPLVKVLGQGDMMVFHLEKMS"
     gene            5529..6305
                     /locus_tag="PLG78_06670"
     CDS             5529..6305
                     /locus_tag="PLG78_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE22830.1"
                     /translation="MIVPIEDLRREIPLAVEKNKTLSMVTYALSDYGEEVLKILVGSI
                     LEKAGRMDLFDIVYTAAKELVVNATKANLKRALFLKLSLNANDPAQYDVGMNYFRGAL
                     TEEKLRKYKKTFKEHNLPVVVTLYYSMDVVHIKVKNNFPLLDIEESRIRQKFRQATSF
                     TSLLDFFMEHGDDTEGAGLGLTMVGILLDESGVDRHAFTLYSSEKYGETVARLEIPLS
                     PDYVSKRKVFESEMHGKGQSPDQLRQDFKYNYKQFTRNHS"
     gene            6311..7372
                     /locus_tag="PLG78_06675"
     CDS             6311..7372
                     /locus_tag="PLG78_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE22831.1"
                     /translation="MSHADSKTRARRVWIQASAGSLSRLGLVSDQIAAPSSDQVQIRV
                     RACGLNFADIFAILGLYSATPSGAFTPGLEFSGEIAALGDIAQQKTGFRPGDRIMGLT
                     RFGAYTDLINVDYRYIRKIPSSWSFEQGAAFLAQGLTAEYAISDLGSVKKGETVLVQS
                     AAGGVGLISLFLLEQLGAHAIAVVGSPSKIDLLVSRGVKRERILLRSASTFRKDLRRA
                     APDGIDVCLDAVYGPFFKPQYDALRAGGRYVLFGAASMMPEGKKPNYLRLAFQYLRRP
                     ALDPLAMISDNKSLLAFNLIWLWDQTDRLAGLADRLLTYPWQNNPPFVGKVFSFEKAP
                     DALRFFQSGESVGKVILSV"
     gene            7369..>8273
                     /locus_tag="PLG78_06680"
     CDS             7369..>8273
                     /locus_tag="PLG78_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE22832.1"
                     /translation="MNADADVVIVGGGMAGLSAGIWAGRLGLKSKIIEKDERAGGQLH
                     WIQDVIPDYPGLELRGTDFARTLCDQAQRSASIETGIAVQKILPFQDAPEAGDLYVLE
                     TTGGILSARAVLISTGLRRRTLPAVRAFHGTGIYYTSQPRDQFKGNSLVIAGGGDGAV
                     ENAILLQAQWKKIYIVHRGAALRARPALADHIRNHSHIEVLLETEIVSVTGDPGIRGV
                     VLSQGGKTRTVDADRLLVKVGFEAENTLLSSGGHHFAHAGSWLDSNDEQMALHSTGDM
                     PLDSRPIWVAGDVCTPRDPSLAVAAG"
BASE COUNT         1913 a         2046 c         2187 g         2127 t
ORIGIN      
        1 tactaccctg tcagaagttc gcaagggcgc tcggacttcg attacattgt ccgccagaac
       61 aaacacagtg agcctttttc tgcctttctt tctcaacaga cgcagaccct ggaaatccac
      121 gggattcatg tagggtcaat agggtttcgc gcattccttt tgcaaatcat tctcgtcttg
      181 atctgaatca atgtttgtgc accgaatctg tgttagtttg gaatcaggag taaacctgtg
      241 atcctgtctt ctgataccct ttccaaaatt gcgctcagcg cgcctcacgc cacaagggtg
      301 tttgcaaaat atagaatgga cttttgttgt ggcggaggga cgagcctctc tgacgcgtgt
      361 atcggaaaag gcctgaattc tgaatctgtc ctggcggaga tacaggcggc ggctccactc
      421 cggcgaagtg tagaggcgga tgctccattg ggggaggtcg tagatcatgt tttgtccacg
      481 catcacgaat ttacccgcaa cattatggca gaaatcgaaa agttttccgt tcgcgtggca
      541 gcggtacacg gggcgcagca tcccgaaatg atcgtgctcc gcgatttgtt tgcggatttc
      601 aaagcagaaa tggaacttca tctgttgaag gaagagcgtg ttctgtttcc ttacattcga
      661 ctgctggaga atggtgccga gaaggatgcg cccgtctgtt tttcagatgt gggcggtccc
      721 atccgcatga tgctcttcga acatgaaaac gccggcgaag accttcgcaa gatgcgagaa
      781 gtcacttctg actacactct cccgcaggca gcgtgcaaca cctacaggct tctatttcat
      841 aagctccagg aactggagga agacgtgcac ctgcatatgc atctcgaaaa caatttgctc
      901 tttcctcgtg caatggagct ggctgcgaat ccaggttgac gatcatcgca aatcgccctc
      961 tatgggccgg tgactttcgt aatccatcgg atgcttcggt ttctcacaat tgcatgcgtt
     1021 gctctttgct cctgccagtg gctgattcgt ccgggaactg atttcgaaaa gagcccgcca
     1081 ccacctgagc ccgattactc cctgagagaa tcctgggccg ctcttccaga ccgcgcagat
     1141 acagcggact ctgtcccttc tgaagcagac tggaaagacg cacagtccac agcctccgca
     1201 gatgttttct ttgttcatcc cactttgtat tttggcggaa cctggaatgc ggatgttgcc
     1261 gatgcaactg tgaaccggag agtggatgag accactcttc gcaagcaggc cagcgttttt
     1321 aattgttgtg cgaggatcta tgcgcctcgc taccgtcagg cgaccatcgc tgcatttctc
     1381 gacaaaaaga acggtgcgcc ggccctggat cttgcgtatt cagacgtgct tcgtgcgttt
     1441 gattactata tgattcacta caatcagggc aggcctgtca tcattgcttc tcacagccag
     1501 ggaacgcgcc atgcctattt cctgttgaaa gatcgatttg atcgaaagcc tgctctcagt
     1561 cgccttgtgg cagcgtatct cattggaatg gccatccctg aacatcccta tgccaccata
     1621 ccgatttgca agtcggccac agaggttcgg tgttttatta cgtatcgctc tgcaacaccc
     1681 ggaacagaac tgaagcgttt gccgcacgat ccagagccac cgtatgcttg taccaatcca
     1741 gttcactgga agtcggacga ttctctggca gaggcagatc tcaatccggg tggggtttca
     1801 gcgaactttg aaaaagtaga tccaaatgtt tgctctgcgc agtgccagaa tggcgttctt
     1861 ctgatttcaa agcccaaaga gtccggctat cataatattt ctggcaactt tcacgtttca
     1921 gactattcgc ttttttatgt gagtattcgt aagaatgtgg cggatcgaat cgccgccttc
     1981 agatgagtgt tcttgtactt gtactcgtcg tcctattttg ttcgattgct ttgctatttc
     2041 cgcggaagca agccaggagt tcctcgcgca tagccgcagt taagcgaaat agtccggtga
     2101 ttctgagaca actggtctat cgcggtggag actgggacgc caatcctgga tctctgcgaa
     2161 acctggcacg tttcgctagc aactgcgaag cgataaaact ggatgtgcgc gccggagagc
     2221 ctgcaaacgg gcgagatcta ccgggttctg atttttttgt atacgcaacc gggcatcacg
     2281 gttttgacct ggcaggctct gatctaacag gatttctcag ttttgtgcgt gcaggtgggt
     2341 tcgttcttgt ggacgacaat cgcggaatgg acgcagcttt cagacgattt gtcgctcaga
     2401 attttccgga agcaaggctc gaacctgttc gatccggtga ggaagtgttt agaaagccgt
     2461 ataacgttgg atccatgcca aaaatcatga ttcacgatgg ttcaccggca gtgttgtatc
     2521 gcctgaagga aggacaggat ttgaatctgt actacagctt tagttctgac ctgggcgacg
     2581 gctgggagga ttcattgcga cacagggtgc cggaagaaac tcgcaggctg gcgttagaaa
     2641 tgggcacgaa cttaattgcg cttgcgttcg cctggtccag cgccgcgcgc gaaagaagag
     2701 ggaatttcgc agcgcgaaat tcctcgtagg aaacgacgct gctcttcaga gcagcgcaac
     2761 ttcagattac tttttcagaa tgacccagat ggcgtggatg attcctggaa cgtagccaaa
     2821 gatggtcaaa agaatgttga gccagaaatg aaatccaatt ccaacctgga ggaaaactcc
     2881 gagaggtgga agtacgattg cagcgatgat gcgaactatg tccatgtgta tctccttatt
     2941 ttactacttg aaaggtcgtt gagtattctg acgtatcacc atcttccgtg tgaacataga
     3001 tggtgatctt ccacgtgccc agatacttat cgtcatgcgt attgggcgtc atccagaagt
     3061 aaacatagtt ccagtctgcc tgtgtatcaa tgtctatgtt gattggtttg ttctttcgcg
     3121 tatcgtcttg tagttcaaag acagcgttga gtttgtacac gcccggtgtt tcgatattgg
     3181 agaagacatg aatcttgtct gccgcgcgaa attttgtctt acatccgctg ggtgttcctg
     3241 cgttctcatc tacggcgagg caagcctttg gtttggatag aattgcttcc tgggcaaaga
     3301 gcgcatggga agaaaagaac agcgccattg tcaataatag tggtttcata gtccctccag
     3361 atttgagact gattttgttt tgcacgagtt caattaaaaa acgtccactc ggatggcgtg
     3421 gttgggacat acatgcacgc attgtccgca accggtgcaa gactttgcaa tgtcaggcag
     3481 cctgtctttt ccgagggaga tcgctccttt cactgggcat tcgcgcacgc aaatcttgca
     3541 tgtttgcgat ttttgacccg gagcgaggcg tggttttctg gactcgtggt ttctgcagag
     3601 cgattctgat accgcggcca cgccgagtcg cgggttttgc ttctttctga gtttcttcag
     3661 cgcgcctgtt tcgcacgaat caatgcacgg catgtcgggg caaagtctgc atgcgtttag
     3721 atttggatcg agaagtggac cgctggccgg tcccatctgg aagagcactc cgtatgggca
     3781 ggcgacgatg caatcgccgc attttgtgca cgccttgttg aatttctttg gatctttcag
     3841 tgcgcctgga ggacgagcga gtttgcgcgg aggaggagca tctgcgggag gaacaggagc
     3901 aggcggagtg aagtgttccg gcgaaagcag atttgaaaat gcaagcagtt gcttgtcgat
     3961 ggcggagccg acgggagtct tcatgacttc ccgcgcgact tcttttacgc cttcgcgaaa
     4021 gaacttacgt cgatccatcc gatggcccag cgcttgcctc tggactcttt ccaggatcgt
     4081 ctccggggac ctgaccctga ggagtgagtt tgtctacgat gcccggtaga atctgaccca
     4141 ggaatttgga agcgttctca ggagatactc ctgccttctg tgccagagcc tggagctgtt
     4201 cgcttcccag gacattcttg atttgttcag gcgaaatcaa tttgttttca ccagttccaa
     4261 tccaggattc tgccaggtct ccgagccctt ttgtcttcag gtcctgaacg attccgccca
     4321 ctcccttttt ctggagcatg tcgatcactc cacccaccag gttctgattg ccttcttttc
     4381 ctgcgacatc attggccgca gagatcactt tatcgaggaa gcccatcctt ctttctggac
     4441 catgcgggtt gatttgcaac ccaatttcgt cgccggatcg cccccgaaaa gggtgaatga
     4501 atcctgaaat cctccgaaga tactatatgc aggtactgga agcaccgaag aaaaaacgca
     4561 gagcaccgaa gaagtcaaaa gatccgtttc atcctccctt gttcgaagcg cgtccaggcc
     4621 acgttcgcct ggtgccttgc aatgcatgtg gtggaatgag ctttaagact ctttttgaaa
     4681 aggagtcttc gctcaaagaa gtcttccagg tggttcgctg caagtgcgga ctcgtgcagg
     4741 taaatccgca gcctgatctc caggcagtcc ttccttacta cgcggattcc tatttcaaga
     4801 agcgcacaga ccgtggatac gataactact tctccgacga aatcaaagca gaaatccgtc
     4861 gcgttttcac aatgaatctc gaggacatcg gcttcttcgc atatgaaaaa aaggtattcg
     4921 agaaacaacg ccctggtcgc gctctggacg caggctgtgc tgcgggatac ttcgtcgaat
     4981 atatggcaga acgtggatgg aatgcagagg gcattgagct ttctgctgtg gcggcaaagc
     5041 caggcaaagc tcgcggcctc cgcatcaccg tcggcgattt tctcacgcac tcggatttga
     5101 ttcctcaatc gtacgatttc atttctttct gggcttccat tgaacacatg catgaacccg
     5161 cgcgcgttct ccttcgcgcc agtgagctac tgcagccggg cgggcgcatg gtgctttcca
     5221 cctgcaggta tggtctgctt gcacgcctgg cggggcggga ctggcgattc atgaacgttc
     5281 cagagcatct gtacttcttc agcgtaaagc gcctaacgcg tcttgcgtca gagcttggct
     5341 tcaaagtcgt aggaaaagtc acatacgggt ctggccttac gaccaggaag gacgcatccc
     5401 cattctatcg ctttgcaaag agatggctcg atcctctcgt caaggtactg gggcagggcg
     5461 atatgatggt atttcacctg gaaaaaatga gttgatcgcc ggagaggggg ctccttaaaa
     5521 tatcacgctt gatagtacca atagaagatc tccgccgcga aatcccgctg gcagttgaaa
     5581 aaaacaaaac tctgagcatg gttacctatg ctctctcaga ctacggcgaa gaagtactca
     5641 agatactcgt cggcagcatt ctggaaaaag ccggcagaat ggatttgttc gacattgtgt
     5701 acacggccgc caaagagctt gtggtcaacg caaccaaggc gaatctaaag cgcgcgctct
     5761 tcctgaaact cagtctgaat gcaaacgatc cggcacaata tgatgttggt atgaattatt
     5821 tccgcggagc tctaacagaa gaaaagcttc gcaagtacaa gaaaacgttc aaagagcaca
     5881 atctaccggt agttgtaact ctttactatt ctatggatgt ggttcacatc aaggtgaaga
     5941 ataactttcc ccttctggat attgaagaaa gcagaattcg ccagaagttt cgtcaggcaa
     6001 ccagtttcac cagccttctt gatttcttca tggaacacgg agatgacact gagggagcag
     6061 gccttggtct caccatggtg ggcatccttc tagacgaatc aggcgtggat cgccacgcat
     6121 tcactctgta ttcaagcgaa aagtacgggg agacggtggc caggcttgag attcctcttt
     6181 cgcctgatta cgtttccaag agaaaagtat ttgagtcgga aatgcacggc aagggccagt
     6241 ctccggatca gttgagacag gactttaaat acaactataa gcagtttacc agaaaccatt
     6301 cgtagacttg atgtcccacg cagattcaaa gaccagagcg cgccgtgtct ggatccaggc
     6361 ttctgcggga tcattgtcca gactgggtct agtgtcagat caaatcgcag cgccctcatc
     6421 ggatcaggtt cagattagag ttcgtgcgtg tggtttgaat tttgcggata tctttgcaat
     6481 tctgggtctt tactccgcca ctccatccgg tgcttttact cctggccttg aattttctgg
     6541 agaaattgca gcgttaggcg acattgctca acagaaaaca ggatttcgcc cgggcgatcg
     6601 gatcatgggt ctcacccgtt ttggcgcata cacggatctg atcaatgtgg actatcgtta
     6661 cattcgcaaa attccatctt cgtggagctt tgagcaagga gctgcatttc ttgctcaggg
     6721 tttgactgca gagtacgcga tttctgatct gggttccgtg aagaaaggag aaactgttct
     6781 ggttcagtct gctgcaggcg gtgttggtct gatttctctc ttcttgcttg agcaattggg
     6841 agcacatgcg attgcagttg tgggaagtcc atccaagatc gatcttcttg tttctcgcgg
     6901 agtgaagcgc gaaaggatcc ttctacgcag cgcttccaca ttcagaaagg atcttcggcg
     6961 tgcggcgccg gatggaattg acgtttgcct ggatgcggtg tacggtccat ttttcaagcc
     7021 gcagtacgat gcgcttcgcg ccggtggacg ttatgtgtta ttcggcgcag caagcatgat
     7081 gcctgaaggt aagaaaccaa actatctcag actggccttt caatacctgc gaagaccggc
     7141 tctcgatccc ctggccatga tttcagacaa taaaagtctg cttgctttta atttgatctg
     7201 gctctgggat cagacggatc gccttgcggg gctcgcagac aggctcctta cgtatccctg
     7261 gcagaacaat ccaccgttcg taggcaaagt gttttccttt gagaaggccc cggatgctct
     7321 tcgcttcttt caatcaggcg aaagcgtggg caaggtcatt ctgtccgtat gaacgcggat
     7381 gcagatgtag tcattgtcgg tggcggcatg gcaggattgt ccgccggaat ctgggccgga
     7441 aggcttgggt taaaatctaa gatcatagaa aaagacgagc gcgccggcgg gcaattgcac
     7501 tggattcaag acgtaattcc tgactatccg ggattggagc tgcgtggaac ggactttgcc
     7561 aggacgttat gcgatcaagc gcagagatct gcttcgatcg aaacaggcat cgcagtgcag
     7621 aagatccttc catttcaaga cgcgcctgag gccggagatc tctacgttct tgaaacaaca
     7681 ggcggcattc tgagtgcccg cgctgtgctc atctctaccg ggctcagaag aagaaccctt
     7741 cctgcagtgc gcgcttttca cggaactgga atttattaca cgtcgcagcc gcgagaccag
     7801 ttcaaaggaa actctcttgt gattgcagga ggtggagacg gggccgttga gaacgcgatt
     7861 ctgcttcagg cgcagtggaa gaagatctac atcgtgcaca gaggagccgc acttcgtgca
     7921 agaccggcac tcgcagatca cattcgaaat cattcgcata tagaagtgtt gctcgaaaca
     7981 gaaattgtgt ccgtgacggg agatccaggt atcagaggtg ttgtgctcag ccagggcggc
     8041 aaaacgcgaa ctgtggatgc ggatcggctt cttgtgaaag ttggatttga ggcagaaaac
     8101 actcttcttt cttccggagg tcatcatttt gcacacgcag gcagctggct ggattcgaac
     8161 gacgaacaga tggccctcca ttccacagga gacatgccac tggactccag gcccatctgg
     8221 gtagccggtg atgtttgcac tccgcgtgat ccttctctgg cagtcgcagc agg
//
LOCUS       DAOQJL010000110         8245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4692_length_8245_cov_3.509158, whole genome shotgun sequence.
ACCESSION   DAOQJL010000110 DAOQJL010000000
VERSION     DAOQJL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_8245_cov_3.509158"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="carB"
                     /locus_tag="PLG78_06685"
     CDS             complement(<1..1198)
                     /gene="carB"
                     /locus_tag="PLG78_06685"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNE22833.1"
                     /translation="MPRRTDLRSILIPGSGPIIIGQACEFDYSGTQACKVLRKEGYRT
                     ILVNSNPATIMTDPGLADATYLEPLTPEFLMQIIEKERPDAILPTVGGQTALNLAMNL
                     WKSGFLQKMGVEMIGANAEAIHRAEDREEFKKAMIRIGLKVPLSVTCETMQTARDFKQ
                     KMGLPLIIRPSFTMGGTGGGIAFTEAEFESICQGGLNASPVHQILVEQSILGWKEYEL
                     EVMRDKADNVVIICSIENLDPMGVHTGDSITIAPQQTLTDMQYQVMRDAALKIIREIG
                     VETGGSNIQFAVNPKDGEMTIIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDE
                     IPNDITKITPASFEPTIDYVVTKIPRFAFEKFPGSNTTLGSMMKSVGETMALGRTFKE
                     SFQKAFR"
     gene            complement(1256..2293)
                     /locus_tag="PLG78_06690"
     CDS             complement(1256..2293)
                     /locus_tag="PLG78_06690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE22834.1"
                     /translation="MAEKKGAIGRLVQKGLDSSIEKARQKGQERLTIMLIPHGQEKIF
                     SLQLNYQMIFFLLGILFLVVGLSFYGFYRKAQKELDADRLRSQYGLNFRGAMTLKEIS
                     GDLGDLNETLYSNLKEIASLTGVPDEEIQELPEISSSYSRANNQLEQEIIQKSKLGAG
                     RNFLPPVYSLKRLTYAFSDQVRALSTVERLVGQGIGLYSAMPMGRPFTEKEMLALRDT
                     SGFGMRVDPFTNAALEMHAGQDIAGPDGTPVRATGTGEVQSTGYDMGYGNRIVVKHGS
                     AYYSTYSHLQRILVRPGEIVRRGQVIGQMGRTGRVTGSHLHYEVWVGDQRRTDPVPYM
                     CAYDFRSDICR"
     gene            2378..3970
                     /gene="lysS"
                     /locus_tag="PLG78_06695"
     CDS             2378..3970
                     /gene="lysS"
                     /locus_tag="PLG78_06695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNE22835.1"
                     /translation="MASKEKSNAQKKPQQAPKPAPEAAPEHDASDLFETRLRKLEEAR
                     ASQNPYDISYQRKNTVSEPASVAVDSIAEGTPGSLVVYNVAGRIRAKRLMGKAGFLDL
                     EDETGRLQIYGTEKDASLDFNTFVSLDLGDIAGFEGTLFVTRTGQISLRATKMTLLAK
                     CLRPLPVVKEADGKVFDAFTDKESRYRMRYVDLIVNPHVRKTFLIRSQIVSQIRSFLN
                     AEGYLEVETPMMHPIPGGASAKPFTTHHNALDMELFLRIAPELYLKRLLVGGFSKVYE
                     INRNFRNEGISFKHNPEFTMLELYEAYGDMNSMLDLCERLFVDLAERVIGSTKLPFEE
                     DFIELKRPWERLTYFDAIKKYAKIDLKENMTGEQAVAAAKAAGLRDEELAHADSVWTV
                     AECIFDARVEEKLMQPVFITHHPIAVSPLAKADPANRNFALRFETYIAGRELCNAFSE
                     LNDPIDQKARFQAQVELREKGSEGGGYMDHDYIRALEYGMPPAGGMGIGIDRLVMLFT
                     NSHTIRDVILFPVLRPETAGSD"
     gene            complement(3971..5446)
                     /locus_tag="PLG78_06700"
     CDS             complement(3971..5446)
                     /locus_tag="PLG78_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE22836.1"
                     /translation="MLSLINWISDLLKAGAANADLEKTRARIDAVFAAQSERRPHVAR
                     TNAKQRIVKLKAMLQWIYAHRDDIHKALRADFQKPEAETDLSEILVVTSEIKHAISHL
                     RRWMKPRRVGASLTFLTTSSSVQFEPRGVVLILSPWNFPFNLTMGPLVSAVAAGNCVM
                     IKPSEFTPATNAVMRQLISEIFPEEEVAMFEGDKEVATLLLDKPFHHIFFTGSPAVGR
                     VVQTAAAKHLSTVTLELGGKSPVIVDKSVDMADAGKKISWGKFMNNGQTCIAPDYALV
                     HKDRYAEFIEGMKKTVASYYGKSEEERKQSPHYARIISSRHHARLSDMVAKSVEQGAK
                     LEFGGITDTTEQYIAPTLLSDVPLSAPVMQEEIFGPILPVIPIDSLEEGLGIVSKLEK
                     PLALYFFSKSNRHIKYVLSETTSGGTCINETLIHFLHPNLPFGGVNFSGSGNSHGFYG
                     FRAFSHERAVLKHHRFAPMKLMFPPYTNTVRKLIEFVVKYL"
     gene            complement(5523..7364)
                     /gene="thiC"
                     /locus_tag="PLG78_06705"
     CDS             complement(5523..7364)
                     /gene="thiC"
                     /locus_tag="PLG78_06705"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNE22837.1"
                     /translation="MQSEIKQNISGPRSKDNPAFSRIYVAGDRYPELRVPFLRVDQSP
                     TQTARGLEPNPAIILYETSGAWGDSAPSIREGHPSPRSKWIIDRGDTVETEARIRPES
                     TVPVFPGRRTRRVAAPGANVSQMHYARKGIVTQEMEFIAIRENTTRAMMRENGNHPGL
                     AKITGQHPGQSFGALTPEITPEFVRDEVARGRAIIPANINHVELEPMIIGRNFLVKIN
                     SNIGTSAVASSIEEEVEKMVWSIECGADTVMDLSTGKHIHETREWIIRNSPVPIGTVP
                     IYEALEKVGGVAEDLTWELYRDTLIAQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVS
                     RGGSILAKWCLSHHRENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDRAQFG
                     ELETLGELTKIAWKNDVQVMIEGPGHIPMHMIKENMDLQLKHCDEAPFYTLGPLTTDV
                     APGYDHITSAIGAAMIGWYGCAMLCYVTPKEHLGLPDKKDVRDGVIAYRIAAHAADLA
                     KGHPGAQIRDNALSKARFEFRWKDQFNLSLDPATACAFHDETLPAENAKSAHFCSMCG
                     PKFCSMKITEDVRKYAEEKGLAAESALETGLAEKSAEFRDKGAEVYL"
     gene            7443..>8245
                     /gene="mdh"
                     /locus_tag="PLG78_06710"
     CDS             7443..>8245
                     /gene="mdh"
                     /locus_tag="PLG78_06710"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNE22838.1"
                     /translation="MSRKKLAMIGAGQIGGNLALLAAQKELGDVVLFDVVEGVPQGKA
                     LDLYESTPVEGCNSIITGTNSYADIAGADICIVTAGLPRKPGMSRDDLINTNTKIVKS
                     VAEGIKQYAPNAFVIVITNPLDAMVHVMKKITGFPKNRVLGMAGVLDSARFRTFISME
                     TGVSVEDITAFVLGGHGDTMVPLPRYSTIAGIPLPEYPGMTPEKIDAMVKRTANGGGE
                     IVNLLKTGSAFYAPASSAIAMAESILRDQKRVFPCAVLCEGEYGHNGIF"
BASE COUNT         1895 a         2033 c         2121 g         2196 t
ORIGIN      
        1 atcggaatgc tttctggaag gattctttga acgttctgcc cagggccata gtttcgccca
       61 cggacttcat catacttccg agagtagtgt ttgagcccgg aaacttttca aaagcaaagc
      121 gcgggatttt agtaactacg tagtcaatcg tgggctcaaa gcttgccgga gtgatctttg
      181 taatgtcatt tgggatttcg tcgagagtgt aaccgacagc gaggagtgct gcgatcttcg
      241 cgataggaaa gccggttgct ttggatgcaa gcgcacttga acgcgatacg cgcggattca
      301 tttcgatgat tgtcatttcg ccgtcttttg gattcacggc aaattgaatg ttggatccgc
      361 ctgtctctac gccaatctct cgaatgatct tgagcgctgc atctcgcatg acctggtatt
      421 gcatgtccgt cagagtttgc tgcggcgcga ttgtgataga atcgccagtg tgcacaccca
      481 taggatccag gttttcaatg gagcaaatga ttacgacgtt atccgcctta tctcgcatca
      541 cttccagttc gtattctttc cagccgagaa tactttgctc tactagaatt tgatgaacag
      601 gacttgcatt gagcccgccc tggcagatgc tttcgaattc cgcttccgta aaagcgattc
      661 caccaccggt tccgcccatg gtgaagctcg ggcgaatgat caggggaagc cccatctttt
      721 gtttgaagtc cctggcagtc tgcatggttt cgcaggtaac gctgagcgga accttcagac
      781 cgatccggat cattgctttt ttgaattctt ctctgtcttc tgcgcggtgg atcgcttccg
      841 cattggcgcc gatcatttca acgcccatct tttgcaagaa gcctgatttc cagaggttca
      901 tggccagatt gagtgcggtc tgtccaccga cggtgggaag aattgcgtct ggcctctctt
      961 tctcgatgat ttgcatcagg aattcaggag tcagaggttc gaggtaagtt gcatctgcga
     1021 ggccggggtc cgtcatgatg gtcgccggat tagagttcac caggatggtg cgatagcctt
     1081 ctttcctcag gaccttgcag gcctgggtgc cactgtaatc aaactcgcag gcctgcccaa
     1141 tgatgattgg gccactgcct ggaatcaata tgcttcgcaa gtcagttcgg cgtggcatgg
     1201 caaacgctga cagaattttg ccagggccta tggtgccaac tggaatttca aagtttcaac
     1261 ggcagatgtc gcttctaaag tcgtaggcac acatgtatgg caccggatcc gttcgccttt
     1321 gatcgcccac ccagacttcg tagtgcaaat ggcttcctgt gactctcccg gttcgtccca
     1381 tctggcctat gacctgacca cgtcttacga tttctccggg gcgaaccagg attcgctgta
     1441 gatgcgaata cgttgaataa taggcacttc cgtgttttac tacgatgcga tttccatatc
     1501 ccatgtcgta tccggtgctc tgcacttctc cagttcctgt ggcgcgcact ggagtaccgt
     1561 cggggcctgc gatgtcctgg cctgcatgca tttccagggc cgcgttcgtg aatggatcca
     1621 ctctcattcc aaatccggac gtgtcgcgaa gtgcgagcat ttctttttca gtgaaaggtc
     1681 ttcccatggg catcgcagag tagagtccga tcccctggcc tacgagtctt tccactgtgc
     1741 tgagcgcgcg cacctgatca gagaatgcgt atgtcagtct tttgagggag tatacggggg
     1801 gcagaaagtt tcttcccgca cccagttttg atttttgaat gatttcctgt tccagttgat
     1861 tgtttgcacg cgaataggag gaactgattt caggaagctc ctgaatttct tcgtccggca
     1921 cacctgtgag gctagcgatc tctttgagat tggaataaag agtttcgttt agatccccga
     1981 gatctccgga aatttctttg agagtcatgg ctccgcgaaa attcaaacca tactgactgc
     2041 ggagtctatc tgcatcgagc tccttctgtg cttttctata gaaaccatag aaggaaagtc
     2101 ccacgacgag gaagagaatg cccagcaaaa agaaaatcat ctggtagttc agctgcagac
     2161 tgaatatctt ttcctggccg tgcggaatga gcatgatggt cagacgttcc tggcccttct
     2221 gacgcgcctt ttctatagag gaatcgagtc ccttctggac gagccttcca attgcgccct
     2281 ttttctccgc cacatctcta tcatggttga ggttcgattc tgtcaaatac gatcctcttc
     2341 gatttgaccg gcagtgcgat agagacagat ctggtccatg gccagcaaag agaaatcaaa
     2401 cgcgcaaaag aaaccacagc aggcgcccaa acctgcacct gaggctgcgc cagagcatga
     2461 tgcgtcggat ctatttgaaa caagactccg caaactggaa gaagcacgcg cttctcagaa
     2521 tccatatgac atttcgtatc agagaaagaa cacggtatct gagcctgcgt ccgttgctgt
     2581 ggattcgatc gcggaaggaa caccgggctc tctagtcgtt tataatgtag caggtagaat
     2641 tcgtgcgaaa cgtttgatgg ggaaggctgg tttcctggac ctggaagatg aaacggggag
     2701 gcttcagatt tacggaacgg aaaaggatgc gtctcttgac tttaacacgt ttgtttctct
     2761 ggacctggga gacatcgcag gctttgaagg cactcttttt gtaactcgca ctggtcagat
     2821 ttcgctcaga gctacgaaga tgactcttct tgcgaaatgt cttcgtccgc ttcctgtagt
     2881 caaagaggca gatggaaaag tgttcgatgc atttacggac aaagaaagcc gttatcgtat
     2941 gcgttatgtg gatttgattg tgaatccgca cgtgcgaaaa acatttttga ttcgctcaca
     3001 aatcgtttcg cagatccgtt cttttctcaa tgcggaaggt tatctggaag tggaaacacc
     3061 catgatgcat ccaattccag gtggtgcgtc tgcaaaacca ttcacgaccc atcacaacgc
     3121 tctggatatg gaattgtttc ttcgcatcgc gcctgagctc tatctcaaga gacttctggt
     3181 gggaggattt tcaaaagtat acgaaatcaa tcgcaacttt cgaaacgaag gaatcagttt
     3241 taaacacaat ccagaattca ctatgctcga actctatgag gcctacgggg atatgaattc
     3301 gatgctggat ttgtgcgaac gtctgttcgt cgatctcgcg gagcgcgtga tcggttcgac
     3361 taaattgcct ttcgaagaag acttcattga actgaaaaga ccctgggaaa gactcacgta
     3421 ctttgacgcc atcaagaaat acgcgaaaat cgatttgaaa gagaacatga ctggcgagca
     3481 ggcggtggct gcggcaaagg cggcgggtct gcgcgatgaa gagctggctc atgccgattc
     3541 agtctggacg gttgcagaat gcatcttcga cgcaagagtg gaagagaagc tcatgcagcc
     3601 tgtgtttatc acgcatcatc caattgcagt ttctcctctt gcaaaggcag atcccgcgaa
     3661 tcgaaatttc gcactcagat ttgaaaccta cattgccgga agagagctgt gcaatgcatt
     3721 tagcgagcta aatgatccta tcgatcaaaa ggcgcgcttc caggcacagg tggagctgcg
     3781 cgaaaaagga tcggaaggcg gcggttacat ggatcatgat tatattcgcg cgctggaata
     3841 tggaatgcct cctgcaggcg gtatgggaat cggaatcgat aggcttgtga tgctattcac
     3901 caacagccac acaattcgcg atgtgattct gtttcctgtt ctgcgtcccg agaccgcagg
     3961 ctctgactga ttacagatat tttacaacaa attcaatgag ctttcgcact gtatttgtgt
     4021 agggcggaaa catgagcttc atgggcgcaa agcgatgatg cttgagcacg gcacgttcgt
     4081 gcgagaatgc gcgaaacccg tagaagccgt gcgaattgcc gctgcctgag aaattcaccc
     4141 cgccaaaggg aaggttggga tgaaggaagt gaatgagagt ttcgttgatg caggtcccgc
     4201 cggaagttgt ctctgacagc acatacttga tgtggcgatt gctcttgctg aaaaaataaa
     4261 gagccagagg tttttcaagc ttcgaaacga tcccgaggcc ttcctccagt gaatcaatcg
     4321 ggatgactgg aagaatgggt ccaaagattt cctcctgcat cacgggcgcg ctcagtggca
     4381 catccgagag tagagtaggg gctatgtatt gttccgttgt gtctgtgatt ccaccaaact
     4441 ccagtttggc gccttgctct acgcttttgg caaccatatc gctcaaacgc gcatggtgcc
     4501 gactgctgat gatgcgcgca tagtgtggac tttgttttct ttcttcttcg ctttttccat
     4561 agtaggaagc aactgtcttc ttcatccctt ctatgaattc cgcataacgg tctttgtgaa
     4621 ccagggcgta atctggagca atgcacgttt gcccgttgtt cataaatttc ccccaggaga
     4681 tcttcttccc cgcgtccgcc atatccacgc ttttgtctac gatgacaggc gatttaccgc
     4741 cgagttctag agtgacggtg gagagatgtt tagctgccgc tgtctggacc actcgcccca
     4801 cggccggact tccagtgaag aatatgtggt ggaatggttt gtccagtaga agggtggcca
     4861 cttctttgtc tccttcgaac atcgccactt cctcttctgg aaagatctcg gaaatcaatt
     4921 gtctcatcac tgcatttgta gcaggagtga attcggacgg tttgatcatc acacagttgc
     4981 ctgccgccac tgccgatacg agcgggccca tggtgagatt gaacggaaaa ttccacgggg
     5041 aaagaatcag aaccacaccg cgaggttcaa actgaacgga actgcttgtg gttagaaagg
     5101 taagggatgc tcccacgcgc ctgggcttca tccatcttct caagtgagat atggcatgct
     5161 tgatctcaga tgtaaccact agaatctcag aaagatccgt ttccgcttct ggcttctgaa
     5221 agtctgcgcg aagtgctttg tgaatgtcgt ctctgtgcgc gtaaatccac tgaagcatcg
     5281 ctttgagttt gacgattctt tgcttcgcgt tcgtgcgagc tacgtgaggg cgcctttcac
     5341 tctgtgccgc aaacactgcg tcgattcgag cgcgggtctt ttccagatct gcgtttgcag
     5401 cgccggcctt gagcaggtct gatatccagt tgatgagtga aagcatcacc catccccgtg
     5461 ggatcaaacg caaggatcaa ctgaaatagt atttcgtttc tgctctctgg cgcgtcccca
     5521 ggctagaggt atacctctgc gcctttgtcc ctgaattctg ccgatttttc ggcaagaccg
     5581 gtttccagtg ccgattctgc ggccaggcct ttttcttctg cgtatttgcg cacgtcctca
     5641 gtgattttca tagagcagaa ctttgggccg cacatactgc aaaagtgagc gcttttcgca
     5701 ttctcagcag gtagagtttc atcgtggaag gcgcaggcag tggctggatc cagagataga
     5761 ttgaactgat ccttccatct gaattcgaat ctggctttag agagtgcgtt gtcccgaatc
     5821 tgagctcctg gatggccttt ggcaagatca gccgcatgtg ccgctatgcg ataggcgatc
     5881 acaccgtccc ggacatcctt tttgtccgga agccccaggt gttcttttgg ggtcacatag
     5941 caaagcatgg cacagccata ccagccaatc atggcggctc cgatggcaga agtgatgtgg
     6001 tcatatccag gtgccacgtc tgtggtgaga ggtcccagag tatagaaagg agcttcatcg
     6061 caatgcttca gctgcaggtc catattctct ttgatcatat gcatggggat atggccgggt
     6121 ccttcaatca taacctgcac atcgtttttc caggcgatct ttgtgagctc tccgagtgtt
     6181 tccagctctc cgaattgggc gcgatcgttt gcgtccgcaa tggaaccagg tctcagtccg
     6241 tccccgagtg aaaagctcac atcatacgct ttcataattt cgcagatctc ttcgaagtga
     6301 gtgtagagaa aattctctct gtgatgcgaa aggcaccact tcgcaagaat cgatccgccg
     6361 cggcttacga tcccagtcat gcgatttgct gtgagtggaa tgtatctgag aagaactcct
     6421 gcgtggattg tgaaatagtc cactccctgt tctgcctgcg caatcaaagt atcgcgatag
     6481 agttcccagg taagatcttc tgccacaccg cccacttttt cgagtgcttc ataaatgggc
     6541 acggtgccga ttggaacggg tgagttgcgg atgatccatt cacgcgtttc gtggatatgc
     6601 tttccagtcg acagatccat cacagtgtca gcgccgcatt caatggacca gaccatcttt
     6661 tctacttctt cttcaatgct tgaggccact gcactcgtgc cgatgttcga attgattttc
     6721 acgaggaaat ttctaccaat gatcattggc tccagttcta cgtgattgat gttggctgga
     6781 atgatggcgc gaccgcgagc tacttcgtct cttacaaatt ctggcgtgat ctcaggcgta
     6841 agcgctccaa agctctggcc tggatgttgt cctgtgattt tagcaagacc agggtgattg
     6901 ccattttcgc gcatcatcgc acgagtagtg ttttcgcgga tggcgatgaa ttccatttcc
     6961 tgtgttacga ttccctttcg cgcatagtgc atttgagaaa cgttcgcacc aggtgccgca
     7021 actcttctgg ttctgcgacc tggaaaaacg ggtactgtgg attctggtcg aatgcgagcc
     7081 tctgtttcta cggtgtctcc tctatctatg atccatttcg atctgggcga tggatgacct
     7141 tcccgaatgc tgggtgcaga gtctccccat gctcctgaag tctcgtacag tatgatggcc
     7201 ggatttggtt ccagtccgcg cgctgtctga gtgggcgatt gatccacgcg aaggaaaggt
     7261 actctcaatt ccggataccg atcacccgca acgtagatgc gcgagaaggc tggattgtct
     7321 ttcgacctgg gtcccgaaat attttgtttg atctctgact gcatgatgga aagcttctcc
     7381 gcgcggcagt ttctaagcgt ttttgccgca gatactcctc tagaaaaacc aggaaggaat
     7441 ttatgtcacg aaaaaaatta gcgatgatag gcgcaggaca aatcggcggc aacctggcgc
     7501 tgctcgcagc gcagaaagaa cttggagatg tggtcctctt tgacgtagtg gaaggcgttc
     7561 ctcagggcaa agcactcgat ctttatgaat ccactccagt agaaggatgc aatagcatca
     7621 ttaccggcac aaactcatac gcagacattg ccggggcaga tatttgcatt gtaacggctg
     7681 gtttgccgcg caagcccggg atgagccgtg atgatctgat caacacaaac accaaaatcg
     7741 tcaaaagcgt agcagaaggc atcaaacaat atgcgcctaa cgccttcgtc atcgtcatta
     7801 cgaaccctct cgatgccatg gttcatgtga tgaagaagat cacaggtttt ccaaagaacc
     7861 gggtccttgg aatggcaggg gtgcttgatt cggctcgttt tcgcactttc atttctatgg
     7921 aaaccggcgt ttctgtggaa gacatcactg cttttgtcct tggcggtcac ggagatacca
     7981 tggttcctct tcctcgctat tctacaattg cgggcattcc acttccggaa tatccaggca
     8041 tgactcccga gaagatcgat gccatggtga aacgcaccgc gaatggcggt ggagagattg
     8101 tcaatttgct aaaaacaggg tcggcgtttt acgcgcctgc atcttctgcc atcgccatgg
     8161 cggaatccat tctgcgtgat cagaaacgtg ttttcccctg tgcagtgtta tgcgaaggag
     8221 aatacggtca caatggaatc ttcat
//
LOCUS       DAOQJL010000111         8205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4717_length_8205_cov_4.867607, whole genome shotgun sequence.
ACCESSION   DAOQJL010000111 DAOQJL010000000
VERSION     DAOQJL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_8205_cov_4.867607"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="PLG78_06715"
     CDS             complement(<1..1621)
                     /locus_tag="PLG78_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE22839.1"
                     /translation="MSLLHPTRETWAQFDPATRKIFQSTVQFFETKGKRALKKEDHEV
                     IWYTDFLDFLKREHVFHYFLTPSAYGDSTECRWDSARNTAYSEILGFYSLSHWYTWQV
                     SILGLGPIWMSKNEEIKKKTAAKLKDGGIFAFGLSEKEHGADLIGSDMMLVPKDGAWI
                     AQGDKYYIGNGNCAAYVSVFGKTADRREEFVFFAVESNHPKYECTQNVVRSQKYVAEF
                     ILHDYPITDKEILSRGRDAWDASLATVAFAKFNLGLAAIGICTHAYYEAINHAAARKL
                     YGTFVTDFPHIRQLFTEAYARLEAMRFFAYRAKDYMKNSSASDRRYLLFNPMVKMKVT
                     MQGEEVINLLWDVIAARGFEKDMYFESAARDIRMLPKLEGTAHVNMVLVIKFMMNYMF
                     ETQEYAAVPENNGPSEDAFLFAQGSTTKGLEKVIPGDYSKVYGRFSSPNLNLFKSQME
                     DLKAFLSKCASDKEQSKDLDFMLTLGEIFTLVAYGQLVLEQCEIEIAKHSEKKAELED
                     LIDLIFDFMVRDFSKHAVTLYGKPSATAAQVQ"
     gene            complement(1652..3862)
                     /locus_tag="PLG78_06720"
     CDS             complement(1652..3862)
                     /locus_tag="PLG78_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNE22840.1"
                     /translation="MEQPRDLFSYKKYWAERFGRAPVLPMSREEMNELGWDSCDVIIV
                     TGDAYVDHPSFGMAIVGRLLEAQGFRVGILSQPDWTSVEDFKKLGKPNLFFGITAGNM
                     DSMVNRYTSDRKLRNDDAYTPGGEAGKRPDRSVLVYTQRCKEAYKKVPTVIGGIESSL
                     RRVAHYDYWSDQVRRSILLDAKADLLVYGNGERQIVEIAHRLANKEDIQSITDIRGTA
                     FLRASIPENFTIIDSTHIDEPGGKDDGKTNPYLYVPGVPSPGAAEPAAKAEILTEGHE
                     YKHPPLVARENTVIRLPSYDALKEDPVLYAHSSRIVHLETNPGNARALLQNYGDRELW
                     LNPPPIPLTTKEMDGIYEQPFSRMPHPSYQGAKIPAFEMIQHSVTIMRGCFGGCTFCS
                     ITEHEGRIIQNRSESSVLREIEKIRDTSPSFTGYISDLGGPTANMYRLACKSRTIESA
                     CRRPSCVYPDVCPNLNTDHSSLVNLYRRAREIPGVKKILIASGVRYDLAVKSPEWIKE
                     LATHHVGGYLKIAPEHTEKGPLSKMMKPGIGAYDRFKELFDRYSAQAGKEQYLIPYFI
                     AAHPGTTDEDMLQLALWLKKNGFRADQVQAFLPSPMASATAMYYSGKNPLRKVTHTSE
                     EVPIPRGLKIRRLHKAFLRYHDANNWPVLREALRRMGRGDLIGNSKHHLIPTYQPLGT
                     GLAHEGRRGPRSAPSTKDRGNKILTQHTGLPPRAHGPSVSSNRGVSRSRPKKKR"
     gene            complement(3899..4480)
                     /locus_tag="PLG78_06725"
     CDS             complement(3899..4480)
                     /locus_tag="PLG78_06725"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE22841.1"
                     /translation="MFFARGFAKMTSDEIASGAGVSKRTLYKYYPEKDRLIESVMESV
                     REDLGALFAQELAKKEIDPMDRFRAIVLGITAYGSRFSKIFMSDLQKVRPDEVQKMIA
                     FRTERLRSLGSLLREGQKAGVVRKGLDANLSVEILLAAMNGVMTPEFLAGGKYSFATA
                     ADGIFELFFRGVLEPGAVRVQKQRYRKVNNRAN"
     gene            complement(4510..5166)
                     /locus_tag="PLG78_06730"
     CDS             complement(4510..5166)
                     /locus_tag="PLG78_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22842.1"
                     /translation="MILKVQNVTKEFGSPPAHVIRGVSLEVGSGEFLALTGKSGSGKS
                     TLLYIISSLDRPTDGSISVDGKDFASMTEPELHRFRNRHMGFIFQFHYLLPEFTVLEN
                     VLMPAVKSGEVKQKRDRALMLLERFDLSERLSHRPGQLSGGQAQRVAIARALLMEPDF
                     IFADEPTGSLDSANGKIVMDIFREISKGKTAVIYVTHDEDFAKLARRQIHLKDGIIVS
                     "
     gene            complement(5168..6415)
                     /locus_tag="PLG78_06735"
     CDS             complement(5168..6415)
                     /locus_tag="PLG78_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE22843.1"
                     /translation="MISIALRQMMARKRQTALTLLGIVFGSTAYIVMSGILLGFREYL
                     VDQLINNDAHVRISARNEVIHEDSLDDRLLDPGERIRWIVPPSGRRDHARIENPSAWY
                     NRLTSDADVSAWSPQLAAKVIFRRGTISEAGKMIGIFPGRQEKVTVIRSQMIHGEFRD
                     IAEGGNRLIMGEGLRELLGARTSEAVHVTVGRGDAVPFKIVGVFQTGNKAFDDSTCFA
                     YLSDVQRLNRTPGVITDIAVRLRDVSASRAKSDFWARISQENVRSWEETNANFFSIFR
                     LQDAIRYSMSISILVVAGFGIYNILNIVINQKKREIAILRSIGFESRDIVVIFLIQGV
                     ILGVAGGLIGMILGYLVCLKLETLNFSNPLIQTKTGKMMVSYRLATYVQAYLLALVST
                     LVASVLPARSAGRLAPIEIIRGE"
     gene            complement(6412..7206)
                     /locus_tag="PLG78_06740"
     CDS             complement(6412..7206)
                     /locus_tag="PLG78_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE22844.1"
                     /translation="MRGQILKTGSGRIFTGAALLVGALAFASFQSRAKEQIVSVRRST
                     FTEAVYGLGTIKANRVFSLRVGVPSRIRKLYVRAGDEVGQGMPLAEFDEMMPVRAPFS
                     GTISALNFNAEEIVFPQANVMTVVDSKDRYLLVSLEEQGAIRVRKGQRVRASFEGWRR
                     ERFLCEAVGLYPEEGQFQMRAHCNDLPPQILPGMTADVLIEVGKRPNVIQIPVSAIRN
                     GKVKLIRGSNDLEVGVSLGAIEGGWAEVASGDVREGDRVRVPEEAK"
     gene            7411..7833
                     /locus_tag="PLG78_06745"
     CDS             7411..7833
                     /locus_tag="PLG78_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HNE22845.1"
                     /translation="MWSKSYSKKVKGIKASQVWKVWSNVDEWHTWQKDTDYAKLDGPF
                     QAGSTFILKPKGGPKVRIEIVEAHENQGFVDLTRFPLARMYGSHQFVEKNGELEMTTT
                     MSVEGPLAFLWRKIVAEDVANGLEAQTEWLIQKAANAK"
     gene            7823..>8205
                     /locus_tag="PLG78_06750"
     CDS             7823..>8205
                     /locus_tag="PLG78_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE22846.1"
                     /translation="MQNSGSPEKDESPFKHESADDSPGFLLYKITVLWQQKLGAIFAS
                     FGIYQTQYAILASLKYFEEKGEASTQSHLVEHARIDKMTLSKAVRALEDAGYVSRSDS
                     KKDARAVSVKLTARGRKLVSSAIRA"
BASE COUNT         2082 a         2124 c         1939 g         2060 t
ORIGIN      
        1 gctgtacctg tgctgcagtt gcagacggct ttccatagag agtgactgca tgtttcgaaa
       61 agtctctcac catgaaatca aaaatcaaat cgatgagatc ttccaattct gctttcttct
      121 ccgaatgctt cgcaatttca atttcgcatt gctcgagcac cagctgaccg tacgcaacca
      181 gagtaaagat ttctccgaga gtcagcatga aatccagatc ctttgactgt tctttgtccg
      241 atgcgcactt tgaaagaaac gctttcaaat cttccatctg tgatttgaaa agattcaaat
      301 tcgggctgga aaatcgaccg tacacctttg aatagtctcc ggggatcact ttctcaagac
      361 ccttggtggt agaaccctgc gcaaataaaa atgcatcttc ggagggccca ttgttttctg
      421 gaacagcagc gtactcctgc gtttcaaaca tatagttcat catgaatttg atcacaagaa
      481 ccatgttcac gtgggctgtt ccttccagct tgggaagcat tcgaatgtcg cgcgccgcag
      541 actcgaagta catatctttt tcgaaaccgc gcgcagcaat cacgtcccag agaagattga
      601 tcacttcctc tccctgcatg gttactttca ttttaaccat gggattaaag agcagataac
      661 gtctgtcaga agcagaggaa ttcttcatat aatccttggc gcgatatgcg aagaacctca
      721 ttgcctccaa ccgggcgtag gcttctgtaa acaactggcg aatgtgtggg aaatcggtta
      781 cgaatgttcc gtacagtttg cgcgccgctg cgtggttgat ggcttcgtaa tatgcgtgag
      841 tgcatattcc aatggccgcc agacccagat tgaatttcgc aaaagcaact gtagccaggg
      901 atgcatccca ggcatcgcga cccctggata gaatttcttt gtctgtgatc gggtagtcgt
      961 gcaggatgaa ttccgccacg tatttttgtg atcttactac attctgagtg cactcatatt
     1021 tcggatggtt gctttctacg gcgaagaaga cgaattcctc acgtctgtcc gctgttttcc
     1081 cgaacacaga tacatacgcg gcgcaatttc catttcctat gtagtactta tctccctgag
     1141 caatccaggc gccatctttt ggaaccagca tcatgtcaga accaatcagg tccgcgccat
     1201 gttctttttc agacagaccg aacgcaaaaa tgccgccatc tttcagtttc gcggccgtct
     1261 tctttttgat ttcctcattt ttactcatcc agatgggccc gagacccagg atggaaacct
     1321 gccacgtata ccagtgggag agagaataaa aaccgagtat ctcactataa gcagtgtttc
     1381 gcgcagagtc ccatctacat tcggtagaat ccccgtatgc agaaggcgtc agaaaataat
     1441 ggaacacatg ctcacgcttc aggaaatcaa ggaagtctgt gtaccagatc acttcgtgat
     1501 cttctttttt cagcgcgcgc ttccctttgg tttcaaagaa ctgaactgtg gattgaaaga
     1561 ttttacgggt cgcagggtca aactgggccc aggtttccct ggttggatgc aaaagtgaca
     1621 taggcgagtt tcaaaatcaa ccgcgcgcaa gtcatcgttt tttctttggt cgtgatcggg
     1681 agactccacg gttggacgaa acggaaggcc cgtgcgcacg cggcggaagt ccggtatgct
     1741 gggtcagaat cttgttccct ctgtctttgg tgctcggagc ggatcgtggt cctctccttc
     1801 cttcgtgcgc caaaccggtg cccagcggct ggtaagtagg aatcaaatga tgtttggagt
     1861 taccgatcaa atccccgcga cccatccgac ggagagcctc ccgcaaaacg ggccagttat
     1921 tcgcgtcatg gtatctgaga aacgctttgt gaagcctgcg aatttttaga ccgcgtggaa
     1981 tcggaacctc ttcgcttgtg tgcgtgactt tgcgaagcgg attttttcct gaatagtaca
     2041 tcgcagtagc actcgccata ggcgaaggca agaaagcctg cacttgatct gcgcgaaatc
     2101 cgttcttctt caaccagagg gcaagttgaa gcatgtcttc gtctgtggta cctggatgag
     2161 ccgcaatgaa atacggaatg agatattgct ccttacccgc ctgcgcagaa tatcgatcaa
     2221 acaattcctt gaagcgatca tacgctccaa ttccaggctt catcattttt gacagtggcc
     2281 ctttctctgt atgttccggg gcaattttca gataaccacc cacatgatgc gttgccagtt
     2341 ccttgatcca ctcaggcgat ttcacggcaa gatcgtatct aactccggaa gcaatcagta
     2401 tctttttgac tccagggatt tcgcgcgcac gtctgtacag attcacaagc gaactgtgat
     2461 ctgtgtttag attggggcaa acgtccggat aaacgcagga aggtcttctg catgcgcttt
     2521 cgattgtgcg agatttgcac gccaggcgat acatgttcgc cgtaggaccg ccgagatctg
     2581 aaatgtagcc agtgaacgaa ggcgatgtat ctcgaatttt ctctatttcg cgcagtacgg
     2641 aagattctga tctattctga atgattcgcc cttcgtgctc tgtgatggaa caaaaggtgc
     2701 aaccgccgaa acatccgcgc atgatcgtga cggaatgttg aatcatttca aaggcaggga
     2761 ttttcgcacc ctgatacgaa ggatggggca ttctgctgaa cggctgctcg taaataccgt
     2821 ccatttcttt cgtagtcagg ggaataggcg gtggattgag ccagagttct ctatctccat
     2881 aattctgtaa aagtgcgcga gcgtttcctg gatttgtttc cagatgaacg attctcgaag
     2941 aatgagcgta cagtaccggg tcttctttca gggcgtcgta agatggaagt cgtataacgg
     3001 tattttctcg cgcaaccagg ggaggatgct tgtattcgtg tccttccgtg agaatctccg
     3061 cctttgctgc gggctcggct gcgcccggag aaggaacacc aggtacatag agatacggat
     3121 tggtttttcc gtcatccttt ccacccggtt catcaatgtg agtggaatca atgattgtaa
     3181 aattttctgg aatagatgca cgaagaaagg cagtgcctcg aatgtctgta atgctctgaa
     3241 tgtcctcttt gttcgcaagc ctgtgtgcaa tctcaacgat ctgtctttct ccgtttccgt
     3301 agacgagaag atctgctttc gcatccagta gaatggaacg tctgacctga tcagaccagt
     3361 aatcgtaatg agcaactctg cgaagactcg actcaattcc tccgatcact gttggaactt
     3421 ttttatatgc ttccttgcaa cgttgagtgt acacgagcac acttcgatcc ggtcgcttcc
     3481 ctgcctctcc accaggagtg taggcgtcgt cattgcgaag ctttcgatcg gaagtataac
     3541 gattcaccat cgagtccata ttgcccgcgg tgattccgaa aaacaaattt ggcttaccca
     3601 gtttcttgaa atcctctact gaagtccagt ccggctgaga gagaatccct actctgaacc
     3661 cctgcgcttc tagaaggcgg cccacaatgg ccattccaaa gctcggatga tcgacatacg
     3721 catcgccggt tacaatgatg acatcgcaag aatcccagcc gagctcattc atttcctcgc
     3781 gggacatagg cagaaccggc gctcgaccaa agcgttccgc ccagtacttc ttataggaga
     3841 ataaatcccg tggttgctcc acacgataag aaagtcacac cccgtgcacc tgtcatgcct
     3901 aattggcgcg gttgttgacc ttcctgtacc gctgtttctg gacgcggacc gctccgggct
     3961 caagaacacc ccggaaaaac aattcaaaga ttccatctgc agcggtcgca aaactgtatt
     4021 taccgccggc aagaaattcg ggcgtcatca ctccgttcat ggcggcgagc aaaatctcga
     4081 cagatagatt tgcgtcgagt ccctttctaa caacaccggc tttttgccct tcgcgtaaca
     4141 gcgaaccaag acttcgcagc cgttcggtcc taaaagcgat catcttctgc acttcgtccg
     4201 gacggacttt ctgaagatcc gacatgaaaa tttttgaaaa acgagatccg tatgcggtaa
     4261 tccccagcac gatcgcgcga aaacgatcca ttggatctat ctctttcttc gcaagctcct
     4321 gcgcaaacag agcgcccaga tcctcgcgaa cactctccat cactgattcg atcaatcgat
     4381 ctttctcggg gtagtatttg tatagagttc gcttgctcac tccagcaccg gaggcaattt
     4441 catctgaagt cattttcgcg aatccacggg cgaagaacat caggcgaact ctttctagaa
     4501 ttttgtcctt caacttacta tgattccatc tttgaggtga atttgtctgc gtgcgagctt
     4561 tgcgaaatct tcatcatgag tgacgtaaat aacagctgtc ttgcctttgc tgatttctct
     4621 aaagatatcc atgacgatct taccattcgc tgaatcaaga gacccggttg gctcgtctgc
     4681 gaaaatgaaa tccggctcca tcagaagagc tctggcaatg gcgactcgtt gcgcctggcc
     4741 tcctgaaagc tggcccggtc tgtgcgaaag cctctcgctt agatcaaatc tttcgaggag
     4801 catcaaagcg cgatcgcgtt tttgtttcac ttcaccggat tttactgcgg gcatcagcac
     4861 attttcgagc acggtaaact caggaaggag atagtgaaac tggaatatga atcccatatg
     4921 tcgatttcta aaacgatgga gttccggctc tgtcatggac gcaaagtcct tcccatccac
     4981 cgatatgctg ccgtccgtcg gtctgtctaa agaactaatg atataaagaa gagtgctctt
     5041 acccgacccg gacttcccag tgagagcaag aaattcgccg gaacctactt ccagcgacac
     5101 tcctcgaatc acatgcgccg gtggacttcc aaattccttt gttacatttt gaactttgag
     5161 gatcattcta ttctcctcgg atgatttcga tgggagcgag tcgtcccgca gatcgcgcag
     5221 gaagaacgct cgcaacgagc gtggacacaa gcgccagtaa atacgcctgc acgtaagttg
     5281 cgaggcgata cgaaaccatc atcttgcccg tctttgtctg aatcaacggg ttagaaaaat
     5341 ttagagtctc gagcttgaga cagaccagat agcccaggat cattccgatc aaaccaccgg
     5401 caactccgag aattacaccc tggatcagga aaatcactac aatgtcgcgc gattcaaatc
     5461 caatggaacg caggattgcg atttcgcgct tcttttgatt gatgactatg ttcagaatgt
     5521 tgtaaattcc aaaacctgcg actacgagaa tggagatgct catcgaatag cgaatcgcat
     5581 cctgcagtcg aaatatagaa aagaaattgg cattcgtctc ttcccagctg cgcacattct
     5641 cctgcgaaat ccgcgcccag aaatcactct tcgctctgga cgcggaaaca tctcgcaaac
     5701 gcaccgcaat gtccgtgatc acacctggag ttcgattgag gcgctgaacg tcgctgagat
     5761 aggcaaaaca agtagaatca tcaaatgctt tgttgccggt ctgaaaaacg cccacgatct
     5821 tgaaaggaac cgcgtctccg cgacctactg tgacatgaac tgcttcagac gtgcgagcac
     5881 ccagaagttc ccgtagtccc tcccccatga tgagacgatt gcccccttca gcgatatcgc
     5941 ggaactctcc gtggatcatc tgactgcgaa tcacggtgac cttttcctga cgcccgggaa
     6001 agataccaat catcttgccc gcttcgctga tggttcctcg tctgaaaatg actttggccg
     6061 cgagctgcgg ggaccatgca ctcacgtctg catcagaagt cagacgattg taccacgccg
     6121 acggattctc tatgcgagca tggtctctgc gcccggacgg cggaacaatc cagcgaatcc
     6181 tttctcctgg atcaagcaac cggtcatcga gagagtcctc atggatgact tcattgcgag
     6241 cagaaatgcg aacgtgcgcg tcgttgttga tcaactgatc cacgagatat tctctaaagc
     6301 caagaaggat tcctgacata acaatgtagg ccgtggaacc aaatacaatg ccgagtaacg
     6361 tcagagcggt ctgtcttttt ctggccatca tctgtcggag tgcaatggaa atcatttcgc
     6421 ttcctctgga acacgcaccc tgtccccttc tctcacatca cctgatgcaa cttctgccca
     6481 accgccctcg atggccccca gagatacacc gacttccaga tcattcgatc cccggatcaa
     6541 tttcaccttg ccgtttcgaa tcgcagagac gggaatctga attacgtttg gcctttttcc
     6601 gacttcaatg agcacatccg cagtcatacc gggaagaatc tgaggcggca gatcattgca
     6661 gtgcgctctc atttgaaact ggccttcctc tggatagaga ccaaccgctt cgcacaaaaa
     6721 tctttcgcgg cgccatccct caaagctcgc gcgcactctc tggccttttc gaacgcggat
     6781 agccccctgt tcctcaagag acacaagcaa atagcgatct ttagaatcga ccacagtcat
     6841 cacattcgct tgtggaaaaa caatttcctc agcattgaaa ttcagagcag agatggttcc
     6901 cgaaaacgga gcacgcacag gcatcatctc gtcgaattcg gccaggggca ttccctggcc
     6961 tacttcatcg cctgcacgaa catacagctt tcgaatacgc gacggcactc caacacgcaa
     7021 actgaataca cgattcgctt tgattgtgcc cagaccatag actgcttcgg taaaagtcga
     7081 gcgacggacg gaaacgatct gttccttcgc acgagactga aaagaagcaa aggcaagcgc
     7141 acccacgagc aatgccgctc ccgtgaagat tcgcccggag cctgttttta gaatttgtcc
     7201 acgcacagac ccatttacca agtatactat tttagtgtca atagtttacc tctgttttcc
     7261 gggggattcc agccgctatg gatccgcgcg ggtttccgct tcgaaaacgg ggatcagccc
     7321 ccggaccgaa ttcgggttga cagaagcgag ggctgggaat catggtcatt tagtaaaccg
     7381 gtttactaat ttcaaaagac ggagaatttt atgtggagca agtcctacag taagaaagta
     7441 aagggaatca aggcatctca agtatggaaa gtatggtcca acgtggacga atggcatacc
     7501 tggcaaaaag atacagacta tgcaaaactg gacgggcctt tccaggccgg aagtacattc
     7561 atcctcaaac ccaaaggtgg accaaaagtg cgcatcgaaa ttgtggaagc ccacgaaaat
     7621 caaggattcg tggatctcac caggtttccg ctcgcgcgca tgtatgggtc gcaccagttt
     7681 gttgagaaga atggtgaact ggaaatgacc actaccatga gcgttgaagg accactcgca
     7741 tttctatgga gaaaaattgt ggcggaagat gttgcaaatg gcctcgaagc gcaaactgaa
     7801 tggttgattc aaaaggcggc gaatgcaaaa tagcggatcg ccagagaaag acgaaagtcc
     7861 attcaagcat gaatccgcgg atgatagccc gggcttcctg ttatacaaaa ttaccgtttt
     7921 gtggcagcag aagctcggtg cgatcttcgc atcatttgga atctatcaga cgcagtatgc
     7981 gattctagcg tctctcaagt atttcgaaga gaaaggggaa gcttccacac agagccatct
     8041 ggtggaacat gcaaggatag acaaaatgac cctttccaaa gctgttcgcg ctctcgagga
     8101 cgccggatac gtttcgcgca gcgattcaaa aaaggatgca agggctgtgt ctgtaaagct
     8161 gactgcgcgt gggcgtaagc ttgtttccag tgccatccgc gctat
//
LOCUS       DAOQJL010000112         8117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4786_length_8117_cov_4.110022, whole genome shotgun sequence.
ACCESSION   DAOQJL010000112 DAOQJL010000000
VERSION     DAOQJL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_8117_cov_4.110022"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /gene="uvrA"
                     /locus_tag="PLG78_06755"
     CDS             complement(<1..2296)
                     /gene="uvrA"
                     /locus_tag="PLG78_06755"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE22847.1"
                     /translation="MSASVIRVQGAREHNLKGITVEIPRDKLVVITGVSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIYDYFRLLFARAGVQHCHNCGRKIQSVGVDQIVDMVQTLSEGSKLQILSPLISGS
                     KGEHKDVIEQARKDGFVRLRINGEVRDLSEDITLAKNRKHDIEIVVDRIVLKPAVRSR
                     LAEALETALEKSGGMVLVNDGHEDRVYSRKSTCTHCNLSLPELSPRSFSFNSPHGACS
                     KCDGLGVLLEFDEDLVVSDPEQSILDGAMAAWGSSKSYWFIETVHNLGKKLGFEPNTP
                     YEKIPEKIRSQILHGTPGKGKFHFEGVLPSLKRRYMETKSDDVRIWLERYMSEKPCPS
                     CKGKRLRPESLAVKFHSETIDSISEKNIRDILQFFEGLKLHSTEEKIAGRIIKEIRER
                     LSFLHNVGVGYLNLSRSAGTLSGGEAQRIRLATQIGSSLMGVLYILDEPSIGLHQRDN
                     DRLLSTLCRLRDLGNTVIVVEHDEDTIRAADHVIDIGPGAGLGGGRVVGEGRPEDLIA
                     SPESITGMYLSGKRKIDVPATRREGNGKKLVIRRARENNLKGIDVEIPLGKLIAVTGV
                     SGSGKSTLVNDILYEALAAKIGEGRHHPGKHDRITGVEALDRVICIDQEPIGRTPRSN
                     PATYTGLFTPIRDLFSRLPEAKVRGYAPGRFSFNVAGGRCENCEGAGIITIEMHFLPD
                     VYVTCDVCKGKRFNRETLEVKFHGK"
     gene            complement(2311..3912)
                     /locus_tag="PLG78_06760"
     CDS             complement(2311..3912)
                     /locus_tag="PLG78_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE22848.1"
                     /translation="MAKVQTRSISRSIQNGRLGSANGLRKRPGPGGQVGLKIIQKGKK
                     KFFEKVNPATLDILGVYPLCEAAEVDHALDAARHGFERWSKLSLRERSKHLKRFRKQL
                     VKDMNEIVDTICQECGKTEMDAMIEVMVVCEHMKYVEQKGPHFLRDEKRSTGIFVIKG
                     AYVSFHPKGVVGVISPWNYPLVLSMGPITQALMAGNSVVLKPSEVTPKTSMKIAEIAY
                     RAGIPQDVLQVITGDGSTGALLVESPKTDMICFTGSTATGRKIGEVCGRLLKPVILEL
                     GGKAPMLVFDDANLKRAVSGALWGGLSNSGQTCITIDRVFVHESIFDKFIELATVELA
                     HIRQGLRHEMPSIGSMTFPRQIEIVGDQVTDAIQRGARIISGGKKNTSYAGHFYEPTI
                     VTNHTPDFRIMREETFGPVINVLPFRTEEEAVKLANDTVYGLNAYVWTRDKKRAKRVA
                     RQIRSGNLNINDVYVNYFMSDLPFGGVKSSGIGRVYAIEGLRAFADQQSVCYDRLGLN
                     KDPYWFPYTPAKQGLIYRATKLLFG"
     gene            complement(3929..5512)
                     /gene="murJ"
                     /locus_tag="PLG78_06765"
     CDS             complement(3929..5512)
                     /gene="murJ"
                     /locus_tag="PLG78_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE22849.1"
                     /translation="MASTARNSLRLSLVTIVSRITGLIRDHYQAVFFGTGTVSAAWEV
                     AYMLPNMLRNLLAEGVLSQAFVPIYSDSLKESEDRARRVSGVILSFLFFVLLGVVFLG
                     ALVFPFLLPLYTGRSGKDAELVIWLATVLFPFILTTSLASIFMGMANTRQSFFVPSLS
                     PVLLNLILIGGFLVLVPFHLESVVSVKWLALITIFGGVSQLAFQAWHIARNGWSPEYN
                     LNLKDPALRKIYTLMAPAGLGASIFQLNQLLDVAIASYMIPEPGAITGLRFAHRLIQL
                     PTGVVGVALSTAILPVLAKAIREDRRSEIPHELSHALSFSFFLNVPAGIGLYLLGQPI
                     IDLLFYGGNWNQASTATTWAALQFYCLGIPLYSANRILTSSFYAHQDTKSPVRILVGV
                     VIINFALNMALVPFLHQGGLALSTSICAAINFIFLYNTLKRHIPEIVLSEAIRSLARQ
                     VPAYGILAIWLLGLREYSDSIAWLGNALGFKGPRALALPLVALGAGAGGVIYVACGFL
                     LRVPELDFLRRRFRLTRGS"
     gene            complement(5470..6318)
                     /locus_tag="PLG78_06770"
     CDS             complement(5470..6318)
                     /locus_tag="PLG78_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22850.1"
                     /translation="MNIATDEQGTLQYMALQGKLDGSTASDFEKTCEHFLHMGARMFL
                     LDARELDYISSSGVGVLIKWARTLERAGGFCAVSGAGSEVKWLLDFVGLSRSYPVFSS
                     EEDARRYLTERHSGATRLSILTTRGPMPESEMRPRAIPVEIRSSAPSDSQTVTSSQTA
                     SIQSTQPAAPLRESAELPAHLETLQKSLERVIHSLERIPDSIREIREQQISIGHAKTE
                     TVRFQDGIVVDCQRCHSHLRIKKSGEHLCPKCGARFLAARDGKVQFSFKNPWPQQQET
                     LSASHS"
     gene            complement(6322..7365)
                     /locus_tag="PLG78_06775"
     CDS             complement(6322..7365)
                     /locus_tag="PLG78_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22851.1"
                     /translation="MKHWFWKTILGWILLFFVILPAVLIPTLYLGKTLGSLYLSETAP
                     AAKIQGLDILKVLSAQAEGDLKPDKLVAISGTMSDVVQRMNTGATPLGIKEIALIDPK
                     GRVLAHNDVTFIAKDAVSPYANDKFLAVLRRPRRDSATVVPLQPYSPALPQNIVAEKL
                     GDRILTPLRSAYPDLFIASYTISSAVYPIDGEAPNAGFIMIVENHAPERFFSILGALL
                     SPVALVTFGSILVAMFFALPVLLLVTARGKQIVYRDHLDDDLPVAHAHAEPETHESID
                     FEDVPLPEFMDEEAASQMAPHANPSTPPATIQHVAPYNDLPPSVSAAAAVMTPPPAHS
                     YDEILDAIPIDAD"
     gene            7428..>8117
                     /locus_tag="PLG78_06780"
     CDS             7428..>8117
                     /locus_tag="PLG78_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNE22852.1"
                     /translation="MKNPELKERFKSWIPHGMEFIGPDQTERILAGCARLREAFAALG
                     AREVIPPLLDFAPTFEMARQSEMASFQTRDMAGELLAFRSDLTVQIIKAAASGVFGQP
                     GNYSYVQTIVLDAAAGSGHRRELLQAGIEFLGPADRRVETMIGLIADIRKDAIIVYGD
                     TSFLRALLSGLSESDFAAAMKILDRKDIQGLRSFCEARSLQNSTAQILCELPFLSGAS
                     LDPLKQAAAGRS"
BASE COUNT         1949 a         2034 c         2119 g         2015 t
ORIGIN      
        1 tcttgccgtg gaacttcact tccagggtct ctcgattgaa tcgctttcct ttgcatacgt
       61 cgcacgttac gtagacatct ggaaggaagt gcatttcaat tgtgatgatg cctgcccctt
      121 cgcagttttc gcatcttccg ccggcaacgt taaagctaaa tctgccggga gcatatccgc
      181 gaactttcgc ctctggaagg cgtgagaaga gatcacgaat gggagtaaaa agaccagtgt
      241 atgtggccgg gtttgatctg ggtgttctgc caataggttc ctggtcaatg caaatgactc
      301 ggtcgagtgc ttctacaccc gttatgcgat catgcttccc tggatggtgc cggccctccc
      361 cgattttggc tgcaagcgct tcgtaaagaa tgtcgtttac cagagtggac tttcctgatc
      421 cggaaactcc ggtcactgca atcagtttgc cgagtggaat ttccacatcg atgcctttca
      481 ggttgttttc gcgcgcgcga cgaatgacca gtttctttcc gtttccttct cttcgcgttg
      541 caggcacatc gatctttctt tttccactga gatacatccc agtgatgctt tcaggagatg
      601 caatcaggtc ttccggcctt ccctctccca ccactcgtcc gccgcccaga cctgctccag
      661 gtccaatgtc gatcacatga tccgccgcgc gaattgtgtc ctcatcgtgc tctactacaa
      721 tcactgtatt tccaagatcc cttaacctgc aaagagtgga aagaagtctg tcgttgtctc
      781 gctggtgaag gccgatcgat ggttcgtcca ggatataaag aactcccatg agagaactgc
      841 ctatctgagt tgcgaggcga atccgttgcg cttctccgcc agacaaagtt ccagcagatc
      901 tggacagatt cagatagccc acacctacgt tgtgcaggaa agaaagcctc tctctgattt
      961 cctttatgat tctacctgcg attttctctt ctgttgaatg aagtttgagg ccttcgaaaa
     1021 actgcaggat gtcccgaatg tttttttcgg aaatggaatc aatggtttcg ctgtgaaact
     1081 tcacggcaag ggactccggt ctcagtctct ttcctttgca ggaaggacac ggcttctcag
     1141 acatgtagcg ttcgagccag atgcgaacgt catccgactt tgtttccatg tagcgtcttt
     1201 tgagagaagg aagcacaccc tcgaaatgaa atttgccttt tccgggtgtc ccatgcaaaa
     1261 tctgggaacg aattttctcc ggaatctttt catatggagt gtttggttcg aatcccagct
     1321 ttttacccag gttgtgaacg gtttcaatga accagtagct tttggaagaa ccccacgcag
     1381 ccatagctcc gtccaggatg ctttgctctg gatcactcac taccaggtct tcgtcaaatt
     1441 caaggaggac tcccaggccg tcacacttgc tgcaggcacc gtgcggagaa ttgaatgaaa
     1501 aacttcgcgg actcagttcc ggaagagaca gattgcaatg agtgcaagtg cttttgcgag
     1561 agtagactcg atcctcatga ccgtcgttta cgaggaccat tccaccgctt ttttcgagag
     1621 cggtttcgag cgcttctgca agcctggatc ttacggccgg cttgaggaca atccgatcta
     1681 cgacgatttc aatatcgtgc ttcctgtttt tagccagggt gatgtcttcg gacagatctc
     1741 gcacttctcc gttgattcgc agtcgcacaa aaccatcttt gcgcgcctgt tcgattacgt
     1801 ctttgtgctc ccctttgctc ccagatatca ggggagaaag aatctgaagt ttgcttccct
     1861 cggagagagt ctggaccata tctacgatct ggtctacgcc tacagactgg atcttccttc
     1921 cgcaattgtg acaatgctgc actccagcgc gtgcaaacaa tagtctaaaa taatcataaa
     1981 tttctgtaac tgttccaaca gtggatcgcg gatttctgtg agttgtcttt tgttcaatgg
     2041 agatagccgg actcaatcct tcgattaagt ccacgtctgg cttctccatc tgacccagaa
     2101 attgcctggc atatgcggaa agtgattcca cataacgtct ctggccttcc gcatagatgg
     2161 tatcgaaagc gagagatgac ttgcctgacc cggaaacgcc ggtgatgact acgagcttat
     2221 ctctggggat ctcaactgtg atccccttga gattgtgctc acgcgctccc tgtactcgaa
     2281 tgacgctggc actcatacga gcaggtctac tcaaccgaac agaagtttgg tggcgcgata
     2341 gatcaagccc tgcttggcag gagtgtaagg aaaccagtac ggatccttat tgagcccgag
     2401 tctatcgtaa catacggatt gctggtctgc aaaggcacgc aggccttcaa tcgcataaac
     2461 ccttccgatt ccactggatt tgactcctcc gaatggaaga tcagacatga agtagtttac
     2521 gtagacgtca tttatgttca gatttccgct gcgaatctgt cttgcaacgc gtttggcgcg
     2581 cttcttatcg cgggtccaga cgtacgcatt gaggccatag acggtgtcgt ttgccagttt
     2641 gactgcttct tcttccgtgc gaaatggaag cacattgatg actgggccga atgtctcttc
     2701 gcgcatgatt ctgaaatcag gcgtatgatt ggtgacgatc gttggttcgt agaagtgtcc
     2761 ggcgtacgac gtattcttct ttccgccgct gatgattcgc gctcctctct ggattgcatc
     2821 tgtcacctga tctcctacaa tttcaatctg acggggaaaa gtcatcgagc caatgctcgg
     2881 catttcatga cgcaaaccct ggcgaatgtg agcgagctct acagtcgcca gttcgatgaa
     2941 cttgtcaaag atggattcgt gcacgaagac tcgatcaatg gtaatgcaag tctggccgga
     3001 gttggaaaga cctccccaca gagcaccgct cactgcacgc tttagatttg catcatcgaa
     3061 tacaagcatg ggtgcttttc caccgagctc gagaatgact ggtttgagca atctaccgca
     3121 tacttctccg atttttctgc cagttgctgt agagcctgta aaacagatca tgtctgtttt
     3181 gggcgattcc accagaagag cacccgtgct tccatcaccc gtgatgacct ggagaacatc
     3241 ttgaggaatt ccagcacgat aggcgatctc cgcaattttc atgcttgtct taggcgtaac
     3301 ttcagatggc ttgaggacga ctgagttgcc tgccatgagt gcttgagtga tcgggcccat
     3361 ggaaagcacg agaggatagt tccacggaga gatgactccc actacaccct ttggatgaaa
     3421 gctgacgtat gctcctttga tgacgaagat cccagtggat ctcttctcat cgcgcagaaa
     3481 gtgcgggccc ttctgctcca catacttcat gtgttcgcag acaaccatca cttcgatcat
     3541 cgcgtccatt tctgttttgc cgcattcctg gcagatcgtg tctacgatct cgttcatgtc
     3601 tttgacaagt tgcttgcgga atcgcttcag atgtttggag cgttcgcgaa gactgagctt
     3661 gctccagcgc tcgaacccgt gacgggccgc atcgagtgca tgatctactt ccgccgcttc
     3721 gcataacgga tatactccga gtatgtctag agtggcggga tttacttttt caaagaattt
     3781 ctttttgcct ttctggatga tcttgagtcc gacctgaccg cctggtcctg gtctcttgcg
     3841 aaggccgttt gcgcttccta gtcggccatt ctggatgctt ctggagatgg atcttgtttg
     3901 gacttttgcc atccttaaag gacgactctc aggaaccacg cgtcaagcga aatcgacgac
     3961 gcaggaagtc gagctcgggc acgcgcaata gaaagccgca ggcaacgtag attacaccac
     4021 ccgccccggc tcccagagca acgagaggaa gcgccagtgc ccttggtcct ttgaaaccca
     4081 gtgcattacc cagccaggca atcgaatcgc tgtattctcg gaggcccaga agccagatag
     4141 ccagtattcc ataggcaggc acctggcggg ccagggaccg gatcgcctct gagagaacga
     4201 tttcaggaat gtggcgcttg agagtgttgt agaggaatat gaaattgatg gccgcacaga
     4261 tagaagtgct gagagccagg ccgccctggt gcaggaatgg aacgagagcc atgttgagcg
     4321 cgaagttgat gatcactaca cccaccagaa ttcgaacagg agactttgtg tcctgatgtg
     4381 cgtaaaagga agaagtaagg atgcgattgg cgctgtagag aggaatgccc aggcaataga
     4441 attgcagtgc cgcccaggtt gtggcggtag acgcttgatt ccagtttcct ccatagaaga
     4501 gaagatcgat gatgggctga ccgagcaagt acagtccgat tcccgcagga acgttcagaa
     4561 aaaaagagaa agacagagcg tgactcagtt catgaggaat ttcagatctt ctatcttcgc
     4621 ggattgcttt tgcgagaact ggaaggatgg cagtggagag agccactcca acaactccag
     4681 ttggcaattg aatcaggcga tgtgcgaatc gtaaaccggt gatggctcca ggttcaggaa
     4741 tcatgtaact tgcaatggca acgtcgagca actgattgag ctggaagatg ctagcgccga
     4801 ggcctgcagg ggccatgaga gtgtagatct ttcgaagggc cggatctttc aaattcagat
     4861 tgtattcggg agaccatcca tttcgggcaa tgtgccaggc ttgaaacgca agctgagaga
     4921 ctcctccaaa gatggtaatg agtgccagcc atttcacgct cacgacactt tccagatgaa
     4981 aaggaaccag taccaagaat ccaccaatca aaatcagatt cagtagaact ggagaaagag
     5041 atggaacaaa gaaagactgc ctggtgttgg ccatgcccat gaatatagac gcgaggctgg
     5101 ttgtgagaat gaaaggaaag agtacagtgg caagccagat gacgagctct gcatctttgc
     5161 cggatcttcc agtgtagagc ggaagcagga acggaaacac aagagctccc agaaacacta
     5221 cgccaagaag aacgaagaat aagaaagaga gtatgactcc gctcacgcgc cgcgcgcgat
     5281 cctctgattc tttcagagag tctgaataaa tgggaacgaa tgcttgcgaa agaacccctt
     5341 ctgcgaggag attgcgaagc atattgggaa gcatgtaagc cacttcccat gcagcggaaa
     5401 ccgttccggt tccaaagaag actgcctggt aatgatcgcg aatcaggcct gtgattcgcg
     5461 atacaatcgt tacgagtgag aggcggagag agtttcttgc tgttgaggcc acggattctt
     5521 aaatgagaat tgaactttgc cgtctctggc cgccagaaaa cgagctccgc actttggaca
     5581 gagatgttct ccagatttct taattctcaa gtgagaatgg caacgctggc agtcgactac
     5641 aattccgtcc tggaaacgta cagtttctgt tttcgcatgg ccgattgaaa tttgctgttc
     5701 ccggatttcg cggatcgaat ccgggattcg ttccagagaa tgaatcactc tttcgaggct
     5761 tttttgcagt gtttcgagat gtgcaggaag ttctgcggat tctctcaaag gggccgccgg
     5821 ttgtgtgctc tgaatggaag cagtctgcga tgaagtgaca gtctgcgaat cagaaggcgc
     5881 ggagctacga atttctacag gaatcgcgcg aggcctcatt tctgattcag gcatgggacc
     5941 tcgcgttgta agaatgctga gccgagtcgc tccagaatgt ctctctgtga gataccttct
     6001 tgcatcttct tcagaagaaa agacaggata ggaacgagag agacctacga aatccagaag
     6061 ccacttcact tcagaaccag caccgctcac cgcacagaat ccgcctgctc tttccagagt
     6121 gcgcgcccat ttgatcaaca cacccacacc cgagctggat atgtaatcca gttcgcgtgc
     6181 atccagtaag aacatccgcg cacccatgtg cagaaagtgt tcgcatgttt tttcaaagtc
     6241 agatgcagtc gatccatcca gttttccctg gagggccatg tattgcagtg taccctgttc
     6301 gtctgtggct atgttcatat ctcaatccgc atcaattgga attgcatcca gaatttcatc
     6361 gtaagaatgt gcaggtggtg gcgtcatcac agctgccgcg gctgagacgg agggcggcaa
     6421 atcgttgtaa ggcgctacgt gctggatggt cgcaggtgga gtggaaggat ttgcgtgggg
     6481 tgccatttgt gacgctgctt cttcgtccat aaactcaggc agaggaacat cctcgaaatc
     6541 aatcgactca tgcgtttcag gttctgcatg cgcatgcgcg actggaagat catcatccag
     6601 gtgatcgcgg tatacgattt gctttccacg tgcagtcaca aggagtaaca caggaagcgc
     6661 gaaaaacatt gcgaccagga tcgatccaaa cgttaccaga gcaacgggag aaagaagagc
     6721 tccgagaatg gagaagaatc tttccggtgc atggttctct acgatcatga tgaatccggc
     6781 gttaggcgct tctccgtcaa tcggatagac ggcggatgaa atcgtgtagg aggcaatgaa
     6841 gagatccggg taagcagaac gcagcggagt gaggattcta tctcctagtt tctccgcgac
     6901 gatgttctgc ggaagagccg gtgaatatgg ctgcagcgga actacggtcg ctgaatcgcg
     6961 gcgaggacgt ctgagcactg cgagaaactt atcatttgcg tacggggaca ctgcgtcttt
     7021 cgcgatgaat gtaacatcgt tgtgcgcaag cacacgccct ttcgggtcaa tcagtgctat
     7081 ctccttgatc ccaagcggag tggcaccggt gttcattctt tgcaccacat cgctcatggt
     7141 tccggagatc gccacaagct tgtccggctt gagatctcct tctgcctggg cggagagcac
     7201 tttgagtatg tcgagtccct gaatctttgc cgcgggggcc gtttcagaga gatagagcga
     7261 acccagggtt ttgcccagat agagcgtggg tatcaggact gcagggagga ttacaaagaa
     7321 cagtaggatc caccccagga ttgttttcca gaaccagtgt ttcatattcg ggcctttctt
     7381 taaaatcggt atttgcgggg gaatagatca taaggaaact ggctatcgtg aagaatcctg
     7441 agctgaaaga gcgctttaaa agctggattc cccatgggat ggaattcatc gggcccgatc
     7501 agacggaacg aattctcgcc ggatgcgcca ggctaagaga agcattcgcg gctcttggtg
     7561 ccagagaagt cattccaccc ctgctcgact tcgcccccac cttcgaaatg gccaggcaaa
     7621 gcgaaatggc aagcttccag accagggata tggccggcga gctccttgct tttcgctcgg
     7681 atttgacggt tcagatcatc aaggcagccg cttccggggt ttttggccag ccgggaaact
     7741 atagctacgt gcaaaccatt gtcctggacg cagccgccgg ctcaggccac aggagagagc
     7801 ttcttcaggc aggaatcgag tttctgggac cagcagatcg tcgcgtcgag acgatgattg
     7861 gcctcatcgc ggacattcga aaggatgcca tcattgtgta cggagacaca tcgtttctgc
     7921 gcgcacttct atctggactc agcgaatcgg acttcgccgc ggccatgaag attctagacc
     7981 gaaaggacat tcagggactg cgcagttttt gcgaagccag atcattgcag aattccaccg
     8041 cgcaaattct ctgtgagctc cctttccttt ccggggcatc tctcgatcct ctcaagcaag
     8101 cagcggcagg aaggtcc
//
LOCUS       DAOQJL010000113         8090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4811_length_8090_cov_4.649160, whole genome shotgun sequence.
ACCESSION   DAOQJL010000113 DAOQJL010000000
VERSION     DAOQJL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_8090_cov_4.649160"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLG78_06785"
     CDS             complement(<1..299)
                     /locus_tag="PLG78_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22853.1"
                     /translation="MLDRLEFTTTKKDGHTIIEIRGEMDVYNSFELKNLMEELSQNGS
                     TKFILKMGALSYIDSSGLGALINQQMRLQKSGGEMFLVSLSDQVRSVLELTKMHS"
     gene            complement(309..2048)
                     /locus_tag="PLG78_06790"
     CDS             complement(309..2048)
                     /locus_tag="PLG78_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22854.1"
                     /translation="MNYRGARMIVGGLTSKNKIDILLYISKEFAYIDNQRDLFDRVVA
                     LSVEIFEVDNVTVRLWQNGQLVPVRFLKETTPPRRPLRPGEGYSGAVFIEKKPVNLDN
                     LEHYPDYLDENESTRCAMVVPILFRDEALGTISIEKDTAYFYKKDDLEILEAMASQLA
                     LALNEVKLIEGLVEARSRIQSDLKMGRSVQSQIIPSEIAPWNSIRFGHYFEPMVEVSG
                     DYFDVMRSGNSLTCIIADVSGHGVPAALVTMALHFQFRRCVEQGFGLTEMMEELNESI
                     RHRLPDGTYFTTQLIRIYADHTYSYVNAGHVRLMHYKRAQKEFIFHDTKGIPLGIAPV
                     RRTDYEEKFSRLEPGDALVMLTDGFYEQRTATHEETGLQTVINWFGEILGAANPPDAA
                     ETTVQFLDRFNKHVEGWPREDDLTLLVLDCSVHLENARKVYLEARKFLREKNLSKAEE
                     LALEAYRLDSSLHDNLLLLSRVYNEQSRPDEAARFLSEYVVSSGAHKPEYYHMLGTLY
                     YRNGDIRAAKRELKKSLSLDHTFTRASLMLARCYLKEGARPKAIKTLQQALKSSPANE
                     QVRQSLKNVESMA"
     gene            2141..3763
                     /locus_tag="PLG78_06795"
     CDS             2141..3763
                     /locus_tag="PLG78_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE22855.1"
                     /translation="MKTAGVLSLKILFAFIVLCMFAGVSSLFADPSGHSTGTAADAMD
                     AQGELFLPQKPAPGADPAAVSAYEDFQRKAATEPLAVKLADSVGQNRIAINLMWTLIT
                     GFLVMFMQAGFALVETGLCRAKNASHTMLMNFLIYVIGLTGFWICGFGLMFGAMPVTS
                     LGGTPGLSPEYEVTIDLFGKTFGLFAWNGFFLTGSSYDVGIYTIFLFQMVFMDTAATI
                     PTGAMAERWNLKSFYVFGFFLSMFLYPIFGNWAWGGGWLSTLGVNFGLGHGYVDFAGS
                     GVVHAVGGLCALAGAIVIGPRLGKYNADGSVNPIPAHHVPMALLGAFILAFGWFGFNP
                     GSTLGVSGGGLNRVGIVAVVTMLAGAGGALSATALIIWKAGRPDPTMVANGMLAGLVA
                     ITAPSGFVSAPIGFLIGAIAGVLVVLSVDFFDHLKIDDPVGAISVHGVCGSFGVLSVG
                     IFADGTANFGGSWNGVAGNVKGAIFGDWGQLFAQLIGVATVIVWSFGVSFIFFKILHK
                     VMGMRVSEQVELDGLDLDETGIMGYPSFPTVE"
     gene            3798..4181
                     /locus_tag="PLG78_06800"
     CDS             3798..4181
                     /locus_tag="PLG78_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE22856.1"
                     /translation="MRQNISTGSKWEPIIGYSRCVKVGNHVYVSGTTASGPNGFVGEG
                     DPYAQTKQIIENISKALEQAGASLKDVVRTRIYMTNIDQWEIVGKAHGEFFGSIMPAT
                     AMVEVKRLISPEILVEIEADAVIQG"
     gene            4162..4695
                     /locus_tag="PLG78_06805"
     CDS             4162..4695
                     /locus_tag="PLG78_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22857.1"
                     /translation="MQSSRAKKSRRLLIVGGLIVAAGIGWGAFRSLNPYVGLKIYKNP
                     ARCYALSIPGHVQIQAEDPSHVTFAAADWTGGIQFIDTKLSVADFAKGYEDRIRKETP
                     RAIFDPTENSSGAVRLTSRTDVEGKTVRTVSVFFPGRKSDIVHAYALIPLPDASGASS
                     FSDRWLSGFVWDSSCTY"
     gene            4727..5548
                     /locus_tag="PLG78_06810"
     CDS             4727..5548
                     /locus_tag="PLG78_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22858.1"
                     /translation="MKNPVPLALTGGLLLAFAFVFLFGQRAQFAGWISKGELAVREEK
                     GTGGPELASLPAPVQTYLGKVLPVKKDIQFVMLKQSGSFWTAPGKDPSSFDAVQYTSV
                     RAPSFSWTAHIQMTPVRVIVVDRLVNGEGALRARVLGLYTVQDASGKEILKGELLRYL
                     AEIPWAPQAILYQKNLFWKPLDATHVELSTNMGDVRAKVVVEFGADGLISRIRAEDRP
                     FKDGDRMIARPWQGMFSDYKEYKGVLLPSHGEVSWILDAGTFKYYSGDLEDYAMY"
     gene            5613..6929
                     /locus_tag="PLG78_06815"
     CDS             5613..6929
                     /locus_tag="PLG78_06815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE22859.1"
                     /translation="MQTLTIARFEDLALAQPIQRAVAEEGYTTPTPIQQQAIPHLLEG
                     RDLLGCAQTGTGKTAAFALPILHRLGLAHKKPTPRHTRVLVLVPTRELAVQVHDSFRV
                     YGKHLHIKTAVVYGGVGQGTQVRSLQTGVDILVATPGRLLDLIDQGYLRLDTLEVFVL
                     DEADRMLDMGFIQPIRQVIEMLPPRRHNLFFSATMPPPIQKLAGSMLVNPVRVEVTPV
                     ASTVEIIDQSVMYVEHDRKRDLLVKLFQDKSLSRVIVFTRTKHGANRVAEMLDKSGTP
                     ADAIHGNKSQSARQRALENFRVGKIRALVATDLAARGIDVDGVTHVINFEIPNESESY
                     VHRIGRTARAGASGKAISLCDDEERAYMKDIERLINKKIRVDESQPYHSDRIANLTNT
                     PRPAPARKGGGGHGHARGKNHPRSSNGRAGSGSSGNRSSRNLGRRR"
     gene            complement(6971..7984)
                     /locus_tag="PLG78_06820"
     CDS             complement(6971..7984)
                     /locus_tag="PLG78_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE22860.1"
                     /translation="MISWSNFAVIARLVMIFRITSLSAVNHEKGVRMSLVALIKPKGP
                     SGFGYGTTAEEVTQGLDLSGKNILVTGCNSGLGEETARVLAKRGARILGTARTVEKAN
                     AAVAPLGNASTGFECELSDPKSVRSCVDAIKKDGVKLDALICNAGIMALPELKQASGY
                     ELQFFTNHIGHFILVNGLLDQLKSNGRVVMLSSSAHANAPREGIQFDNLSGEKSYRPW
                     AFYGQSKFANLLFAKELARRFAGTERTANALHPGVIKTNLARHMNPLAHVAFGVIGPV
                     FLKSIPQGAATQTYVAVHPHAAAHSGKYFADCNIASPRRDAEDADMARKLWDVSEKIV
                     SGL"
BASE COUNT         1800 a         2020 c         2106 g         2164 t
ORIGIN      
        1 aaactgtgca ttttggtcaa ttccagcacg cttcggacct gatcggacag actgacgagg
       61 aacatttctc ctccactttt ttgcagtcgc atttgctggt tgatcagtgc acccaggccg
      121 cttgaatcta tgtagctgag cgcacccatt tttaaaatga actttgtaga tccgttctgg
      181 ctaagttcct ccatcagatt tttcaactcg aaggaattgt aaacgtccat ttctcctcga
      241 atttcgatga tggtgtgacc gtctttcttt gtagtagtaa attccagtcg atcgagcata
      301 tcccctcctc acgccatact ttccacgttc ttcaatgact gccgcacctg ttcgttcgca
      361 ggtgaactct tgagcgcctg ttgtagagtc ttgattgctt ttggtcttgc cccttccttc
      421 aagtagcatc gcgcgagcat gaggctggct ctggtgaatg tatgatccag agagagagat
      481 tttttcaatt ctcgcttagc agcgcgtatg tcaccatttc tatagtaaag agttccgagc
      541 atatgatagt actcaggttt gtgtgcacca ctcgatacta cgtattcgct caggaatctc
      601 gcggcttcat cgggcctgga ctgttcattg tagacgcgcg aaagcaaaag cagattgtca
      661 tgaagagaag aatccaggcg atacgcttcg agagccagct cttccgcctt tgagagattc
      721 ttttcgcgca agaattttct ggcctctaaa tatactttgc gcgcgttttc tagatgcacg
      781 ctgcaatcaa ggacaagcag agtgaggtca tcttcccggg gccaaccctc cacgtgtttg
      841 ttgaaacggt ccaggaattg aaccgttgtc tccgctgcat cgggtggatt tgccgcgccc
      901 agtatttctc caaaccagtt aatgactgtc tgaagtcctg tctcttcatg agtggcggtt
      961 cgttgttcat agaatccgtc ggtgagcatt acgagcgcgt ctccgggctc cagtctgctg
     1021 aatttctctt cgtagtctgt tcttcgcacc ggagcaatgc caagtggaat ccctttcgtg
     1081 tcatggaaga tgaattcttt ttgcgctctt ttgtagtgca tcaggcgaac gtgtccggca
     1141 ttcacataag agtatgtgtg gtccgcgtaa attcgaatca attgtgtcgt aaagtaagtt
     1201 ccgtccggaa gccgatgccg aatgctttcg ttcagttctt ccatcatttc tgtaaggccg
     1261 aaaccctgtt ccacgcatct tctaaattgg aaatgaagtg ccatagtgac gagggctgca
     1321 ggaacgccgt gaccggaaac atccgcaatg atgcaggtca gactgtttcc tgatcgcata
     1381 acgtcaaaat agtctccgct cacttccacc attggttcaa agtaatgacc gaatcgaatg
     1441 ctgttccagg gcgcgatttc agacggaatg atctggcttt gtacactgcg gcccatcttc
     1501 aggtcgctct ggatacgtga gcgagcttcc acaaggcctt cgatcagttt cacttcattg
     1561 agagcgagag ccagctggga tgccatggct tcaagaatct ccaggtcatc ctttttgtag
     1621 aaatacgcgg tatccttttc aatggatatg gtgcccaggg cttcgtcgcg aaagaggata
     1681 ggaaccacca tcgcgcacct tgtactttcg ttttcatcca ggtaatcggg gtagtgctct
     1741 agattgtcca ggttcacagg ttttttctca atgaaaactg ctcccgaata cccttctcct
     1801 ggtcgcagtg gacgacgcgg cggagtggtc tctttcagga atcgcactgg aaccagttgc
     1861 ccgttttgcc agagtctgac tgttacgttg tccacctcaa agatttccac tgagagagcg
     1921 accacccgat cgaacagatc cctttgattg tcaatgtacg cgaattcttt agaaatatat
     1981 agcaatatgt cgattttgtt ttttgacgta agaccgccga cgatcatgcg agcgccgcgg
     2041 tagttcacga ctttcctctt gccatatttc aaagcgatca tgctaaccct tgagcaatat
     2101 ctataccagg gcctggctcg ctttttgcaa aacggcaggt atgaaaaccg ccggcgttct
     2161 atctctgaaa atactcttcg ccttcatcgt tttgtgcatg tttgcgggcg tttcatcgct
     2221 ttttgcggat ccttccgggc attccacagg cacggcggcg gatgccatgg atgcgcaagg
     2281 agaactcttc ttgcctcaaa aaccagcacc gggagcagac ccggctgctg tttccgcgta
     2341 cgaggacttt cagcgaaaag ctgcgacaga acctctcgcc gtcaaactcg cggactccgt
     2401 tggacagaat agaatcgcca tcaacctcat gtggaccctc atcaccggtt ttctggtcat
     2461 gtttatgcag gccggttttg ccctggtaga gacagggctg tgtcgtgcga agaacgcttc
     2521 gcacaccatg ctcatgaact ttttgatcta cgtaattggt ctgacgggat tctggatttg
     2581 cggatttggt ttgatgttcg gtgcgatgcc agttacaagc ctgggcggaa cacctggtct
     2641 gagtccggaa tacgaagtga cgatcgatct attcggaaag acattcgggc tcttcgcatg
     2701 gaatggattt tttctgacag ggtcgtcgta tgacgttgga atctacacca tcttcctctt
     2761 tcaaatggtg ttcatggata cagccgccac cattcccacg ggcgcgatgg cggaaagatg
     2821 gaatctgaaa agtttctacg tctttggttt cttcctctcc atgtttctct acccgatttt
     2881 tggaaactgg gcgtggggag gtggctggtt gtcaacgtta ggcgtgaact ttggactcgg
     2941 acatggctat gttgatttcg caggttccgg agtcgtgcat gcagtcggtg gtctatgcgc
     3001 tctggctgga gcgatcgtaa tcggaccgcg tcttggaaaa tacaatgcgg atggttccgt
     3061 aaatccaatt ccagctcatc atgtacccat ggctcttctc ggtgcgttca ttctcgcatt
     3121 cgggtggttt ggttttaatc caggttccac tctaggtgtt tcgggaggtg gactcaatcg
     3181 agttgggatt gttgcggtgg tgactatgct cgcaggtgcc ggtggcgctc tgtctgctac
     3241 tgcactcata atctggaaag caggcagacc agatccaacc atggttgcaa acggaatgct
     3301 ggcaggcctg gttgcgatca cagctccgtc cggatttgta agtgcgccga ttggttttct
     3361 gattggtgcg attgcgggtg tgctcgtagt gttgtctgtt gatttctttg atcacttgaa
     3421 aatagatgat ccagtgggtg cgatatcagt gcacggagtt tgcggatcgt tcggagttct
     3481 ttccgttggg atctttgcgg atggaaccgc taactttggt ggatcctgga acggagtggc
     3541 cggcaatgtc aaaggtgcga tcttcggaga ctgggggcag ctcttcgcac aattgattgg
     3601 cgtggccacc gtgattgtct ggtcttttgg cgtatcgttt atattcttta agatcctgca
     3661 caaggtcatg ggcatgcgcg tgtccgaaca ggtggagctt gacggtctgg atctggacga
     3721 aaccggaatc atgggatatc cgagtttccc gaccgtggag tgattgcgcg cacagtgcgc
     3781 gatttcatca cggttctatg cgtcaaaata tttcaaccgg ctccaagtgg gagccgatca
     3841 ttggttactc tcgttgcgtg aaagttggca accatgtgta tgtttccgga actacagcgt
     3901 ccggcccgaa tggatttgtt ggtgaaggag atccctacgc tcagaccaaa cagatcatcg
     3961 aaaacatttc aaaagcgctc gagcaagccg gcgcttcctt gaaggacgta gttcgcacac
     4021 gcatctacat gacaaacatt gaccaatggg aaatcgttgg aaaggcgcac ggcgaattct
     4081 ttggatcgat catgcctgcc actgcaatgg tagaagtcaa aaggctgatt tcccccgaga
     4141 tactcgtgga aatcgaagcc gatgcagtca tccagggcta agaagtccag acgacttctc
     4201 atagtcggcg gcctgattgt tgccgccggc attggatggg gagcgtttcg ttctctgaat
     4261 ccttacgtag gacttaagat ctataagaat ccagcgcgat gctatgcgct ttctatcccg
     4321 ggccacgtgc agatccaggc ggaggatcct tcccatgtca catttgccgc ggcggattgg
     4381 actggcggga ttcaattcat tgatactaag ttgagtgtag cggactttgc gaagggctac
     4441 gaagatcgca ttcgcaaaga aacaccccgg gcaatcttcg accctacaga aaattcctcc
     4501 ggggcagtgc gcctaacgtc cagaacagac gtagaaggaa aaacagttcg cactgtttct
     4561 gtcttttttc cgggcagaaa atcggacatt gtgcacgctt acgcattgat acctctgccc
     4621 gatgcctcgg gcgcttcctc tttttctgat cgatggcttt ctggttttgt atgggactct
     4681 tcgtgtacgt attgacgttc gcacggcgca ttccctttgt tgcggtatga agaatccagt
     4741 tcctcttgcg ctcacaggtg gtctattgct cgcgttcgct tttgttttct tgtttgggca
     4801 gagagcgcaa tttgctggat ggatttcgaa aggtgagctg gctgttcgcg aggaaaaggg
     4861 aacaggtggg ccggaactgg cttctctgcc cgcgcccgtg caaacatatt taggcaaagt
     4921 tcttccggta aagaaggaca ttcagtttgt gatgctcaaa caatccggtt ccttctggac
     4981 tgctcctgga aaagatccct cctcttttga tgcagtccag tacacttcgg tgcgtgcccc
     5041 ttccttttcg tggacagccc acatccagat gactcctgtg cgtgtgatcg ttgtggatcg
     5101 actggtcaac ggagaagggg ctcttcgcgc gcgcgtactt ggactctata ctgtgcagga
     5161 tgcttctgga aaagaaatcc ttaaaggtga gcttctacga taccttgcag aaattccctg
     5221 ggctccccag gcaattctgt atcaaaagaa cttattctgg aagccactgg atgccactca
     5281 tgtggagctc agcacaaata tgggcgacgt cagggcaaag gtcgtggtcg agtttggtgc
     5341 ggatggactc atttcgcgca ttcgtgcgga ggatcgtccc ttcaaagatg gggatcgcat
     5401 gattgccagg ccgtggcagg gaatgttctc agactacaag gaatacaagg gagtcttgct
     5461 tccatcacat ggagaagtga gctggattct ggatgcggga acattcaaat actacagcgg
     5521 agacctggaa gactacgcta tgtattgatt tagatatttg aatatctagt atccgccacc
     5581 ggcttccgcg aaaaactgac tcaaactact tcatgcaaac actaaccatc gcgaggtttg
     5641 aagatctcgc actcgcgcag ccaattcagc gcgcggtagc cgaagaaggc tacactactc
     5701 caacacccat ccagcaacag gccatcccgc atctactaga aggtcgtgat ttgctaggtt
     5761 gcgcgcagac cggcactggc aaaacagcag cgtttgctct tccaatcctg catcgtcttg
     5821 gtctggctca taagaaaccc acaccgcgtc atactcgcgt cctggttctt gtgcccacac
     5881 gagaacttgc cgtgcaagtc catgatagct tccgagttta cggaaagcat cttcacatca
     5941 aaactgcagt cgtttatggc ggcgtgggac agggcaccca ggtgcgcagc ttgcagacgg
     6001 gcgtggacat tcttgtcgca acacccggca gactccttga tttgattgat cagggttatc
     6061 ttcgcctcga tactcttgag gtctttgtcc tggatgaagc ggacagaatg ctcgacatgg
     6121 gtttcatcca gcccattcgt caggtgattg agatgcttcc gccgcgccgt cacaatcttt
     6181 tcttttctgc caccatgcca cctcccatcc agaaactggc aggcagcatg cttgtaaatc
     6241 cagtgagagt ggaagtgacc cctgttgcat ccactgtaga gatcattgat caatccgtca
     6301 tgtatgtgga gcacgatcgc aaacgagatc tccttgtgaa gttgtttcag gacaagtctc
     6361 tcagtcgcgt gatcgtgttc acgagaacca aacacggtgc gaatcgagtc gcagagatgc
     6421 tcgataaaag tggaacgccg gcagatgcaa ttcacggaaa caagtcgcag agcgctcgtc
     6481 agcgtgccct tgagaatttt cgcgttggta agattcgcgc tcttgtggcc acagacctgg
     6541 ccgcgcgcgg tatagacgtg gacggagtaa ctcacgtaat caatttcgaa attccaaacg
     6601 aatcagaaag ctatgttcat agaatcggtc gaaccgctcg tgctggagcg tctggcaaag
     6661 caatctccct gtgcgatgat gaagagcgcg cctacatgaa agacattgag cgtttgatca
     6721 acaagaagat ccgcgtggac gagagccagc catatcattc cgatcgaatt gcaaatctta
     6781 caaacacacc tcgccctgcc cctgcaagaa agggtggggg cggacacggc catgcgcgtg
     6841 gcaagaacca tcctcgttcg agcaatggca gagcgggttc tggttcctca ggcaaccgct
     6901 cttcgcgcaa tttgggaaga agacgttagg cgccatttac ggcgcctgat caaataacgt
     6961 tacgcggcat tcacaggccg cttacgatct tctcggaaac atcccacagc tttcgcgcca
     7021 tatccgcatc ctctgcgtcg cgtcttgggc tggctatgtt gcagtctgcg aagtatttgc
     7081 ctgagtgggc tgcggcatgt ggatgaacag caacatacgt ctgtgtggcc gctccctggg
     7141 gtatgctttt gagaaaaaca ggtccgatca ctccaaacgc tacgtgcgcc agcgggttca
     7201 tgtgtcgcgc cagatttgtc ttgatcacac cgggatggag cgcattggca gtgcgttctg
     7261 ttcctgcaaa ccgacgggcg agttcttttg caaagagcag atttgcaaac ttggattggc
     7321 catagaatgc ccacggtctg taggattttt ctccgctcaa attgtcgaat tgaatgcctt
     7381 cgcgaggcgc gttggcatgt gctgaactac tgagcatcac gactcttccg ttgctcttca
     7441 attgatccag tagcccgttt acaagaatga agtgaccaat gtggtttgtg aaaaactgca
     7501 attcatagcc tgacgcttgt ttcagttccg gcagtgccat gatcccggca ttgcaaatca
     7561 acgcgtccag tttcactccg tctttcttga tggcatccac gcaagatcgc acgctctttg
     7621 gatccgaaag ttcacattca aatccagtag aagcatttcc gagtggtgcg acggctgcgt
     7681 ttgctttttc aaccgtgcgc gcagtgccaa ggatgcgtgc tccccgtttt gccagaactc
     7741 tggcggtttc ttctccgaga cctgaattgc agcctgtgac tagaatgttt tttccagaca
     7801 ggtctaatcc ctgggttact tcctctgctg tggttccata accgaacccg cttgggcctt
     7861 ttggtttgat gagggcgacg agagacatac gaactccttt ttcatggtta actgcactca
     7921 gggaagtaat gcgaaagatc atgacgaggc gagcaataac tgcaaaattg gaccatgaga
     7981 tcatctcttc agctgtacag tattctgagc gggcttgcgg cgattctgct cggaatcggc
     8041 attgcactgt ccggtttttc gggtgaagtg caggaatgga tctttctctg
//
LOCUS       DAOQJL010000114         8014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4878_length_8014_cov_4.963815, whole genome shotgun sequence.
ACCESSION   DAOQJL010000114 DAOQJL010000000
VERSION     DAOQJL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4878_length_8014_cov_4.963815"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLG78_06825"
     CDS             <1..204
                     /locus_tag="PLG78_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22861.1"
                     /translation="HESLGLTGEEVFEILGIAQAKPRGKLTVKAGEKSFEVICRLDNP
                     VEIEYYRHGGILQAVLRSFLKGK"
     gene            206..685
                     /locus_tag="PLG78_06830"
     CDS             206..685
                     /locus_tag="PLG78_06830"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNE22862.1"
                     /translation="MNVHDFTIKTITGEEKSLKEYSGKSLMIVNVASKCGLTPQYAGL
                     ESLYKKYKDRGFNILGLPCNQFLHQEPGTESDIQAFCSMNYGVTFDLGSKIEVKGKTP
                     HPLYAFLTGDGAKFPGKISWNFEKFLVGKNGEVLARFSPQTVPEDPSIVSAVESSLS"
     gene            687..1706
                     /locus_tag="PLG78_06835"
     CDS             687..1706
                     /locus_tag="PLG78_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE22863.1"
                     /translation="MRVHNYAAVPDPQKIGTDLYRIVIPQPFYEPNNIYLITSGEPTL
                     IDTGYIENLGLLQRSLRSIGHSLSKIKHIIYTHDHIDHMSAALSIRFYTDARTYAMAG
                     IEPHVANYASNLLLMKRAEERLTYKAHRDPSVRKAELARARKGWDEFTESYAGPGKVE
                     KAVRIDTGLMEGDVIECGSREIGFLYTPGHNRWHITPYIVGEGIYFTGDLVLDNVSSV
                     YAEVDGDLQKYHESLDRLFKIPIQRLLPAHGNEPEDPKKKIRLLQKTLALLERGVIRC
                     LKEKPHDLAELVSSAMGERVRKSGYYFTALGVMHSIIKKLAVQGQIVVHEIDPPYEQY
                     SLKET"
     gene            complement(1693..3288)
                     /locus_tag="PLG78_06840"
     CDS             complement(1693..3288)
                     /locus_tag="PLG78_06840"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNE22864.1"
                     /translation="MRIALAVFLSCTSASFAQSVQYLDQGWKQSDRSWFYRIDQGSEF
                     MRYDFFSRVERADSDALFSAPESLRAYGFVYDEPVKPGDYPIGFVKRGNSIGFTCAAC
                     HTLEITYAEKRIRIDGGSSLANMEGFLNDLSSALDTTLASPEKFSRLAERTLGRENTK
                     PDAVAVLKKYLAQAADERREYNRTNHPDSPYGPARLDAFGRIYNRVLWMAHSKDRVRA
                     NAPVSFPYLWDAPRHDYVQWIGSASNQGAGGLARNVGQVVGVFGTVDLSSLRPGYTSS
                     IQMTNLMQIESRLAQLQSPQWPASFPSLDKAKIARGREIFASTCMSCHADIRRDDPKR
                     KITAEMTSIELLGTDPLTAQNAVNAEADTGILKGRFVHVVGGVTRFGERSSVLDMLRH
                     VVEGLLAQQPDKIVKSSITNVGWFTTERHGQYERDAKNPVRSLLTYKARPLNGVWATA
                     PFLHNGSVPTLYDLLLPADKRPATFKVGSNQYDPEKAGFVSDQGSFLFDTSLRGNSNK
                     GHEYGTSISEADRLALLEYLKSL"
     gene            3426..4112
                     /locus_tag="PLG78_06845"
     CDS             3426..4112
                     /locus_tag="PLG78_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNE22865.1"
                     /translation="MPKSFILSYVEDKMMKDHAGQKDPGPVITIARDYGCPGVPVAEA
                     VTKALTNYYVEWKLIDKEIINHAAQELRVSPEVAERIGHSGPRGFFHDFISAFSQQQD
                     PSDAAVKRTMANAIRAFSLAGNVVILGRGGVVVTRDIKQSLHVKLYGPIDYRISRVKE
                     MDNLKSDKDALQKIGVVDRERVYLRDYLAGEELRPDVFDIQLNCGTLTEQEVIETIVS
                     IARDKFKLER"
     gene            4109..4537
                     /locus_tag="PLG78_06850"
     CDS             4109..4537
                     /locus_tag="PLG78_06850"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE22866.1"
                     /translation="MMSREDAARFLQTERIAVLATVSPDGMPQSALIGIAATPDFELV
                     FDTIKTTRKYRNLAQDPRASLVIGCTESGITLQYEGVARELRGIEMEKYRQIYFAQWP
                     DGTARLSWDGITYFAVRPSWIRYCNYAVEPPVLTEFTFPF"
     gene            complement(4574..5800)
                     /locus_tag="PLG78_06855"
     CDS             complement(4574..5800)
                     /locus_tag="PLG78_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNE22867.1"
                     /translation="MKLSPFAAVRPRSDLAAEVVCLPYDVMNTAEAREMAHGKPSSFL
                     HIIRSEIELPDSADPHTDAVYQLAKKNLETMLSDGRLLRESGPCLYVYRLVKDGRNQT
                     GLAALNHIDDYLQDKIKKHELTRRDKEEDRLHHIDTTGANCEPVFLAFKSGEQPEIAR
                     ILNEVADKEKPLYDVTPEDGVRHLVYSIPVGEKQDKLKSLYESLSCVYIADGHHRSAS
                     TVRVGKARREASGPGSHEWFLAVTFPDADLRILPYNRVVKDLNGLSKEAFFEKVKKSF
                     QLSEGKSNPALHTVHMYLDRQWYTLTAKEETYKGADAIGRLDVSILQNQLLDPILGIA
                     DPRTSERVAFVGGIRGEGELIKLVDSGAWAVAFSMYPTSMQELFDIADAGQIMPPKST
                     WFEPKLRSGFLVHKFS"
     gene            complement(5854..6393)
                     /locus_tag="PLG78_06860"
     CDS             complement(5854..6393)
                     /locus_tag="PLG78_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNE22868.1"
                     /translation="MARTPVKKTTFLEAVAACIQHEKEVFGFYLKQAEALPEGAIKKL
                     FFQLAEDVEEHIRMISEIYSQVKGGEALPNLKMASEVQKFNGTSLQILMRRLDRITGS
                     DGKGNEIEAIALATREHEDAAAFYRKMTDKFEDKGIQVLFGQLANFQDECRLLLESYG
                     AYLTQGTPASQPSAYWDET"
     gene            complement(6459..>8014)
                     /locus_tag="PLG78_06865"
     CDS             complement(6459..>8014)
                     /locus_tag="PLG78_06865"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNE22869.1"
                     /translation="SHYPADYICEAIDQTRGWFYTLLAISTMVSDETSYKNVVCLGHV
                     LDGKGEKMSKSKGNTVDPWQVFEMHGADAIRWHFLVGAPPGNSRKMGKPGTDQDPIGD
                     VFGFFNMLQNSAGFFSLYANVDGIEINEWQKNPVAGALPFKERPDIDRWILSRLQVLI
                     RDVTASMNEYDAQKAGRLVELFTDDLSNWYIRRNRRRFWKGERDAEKLTGFDTLYRCL
                     VTIARLIMPFAPFLGEELYRGLVASQSKSAPISANLAGWPEYDASFFDAKILEEGDLV
                     LQAASLGRAARAASGMKIRQPLSRIRVFAANASAKSAIEKNEDVLKEELNVKSIEFLA
                     DAQGLIDYRIKPNLPRLGKRVGQHMKAVQEYFRSIKDPSTVARAVRAGSSLSLEDQGV
                     KIELEPEDVLVEPVSAEGTAGVEGSGMTVVLDTGLTPELIQEGIVRDLVRNIQEMRKK
                     AGLEVTDRITLVMEADPEVSSAIERFAGYIEDETLAKIEKSHAGKATGESAFDLDGLS
                     VSVKLFSLR"
BASE COUNT         2069 a         2019 c         1989 g         1937 t
ORIGIN      
        1 catgaatccc ttggactcac cggagaagaa gtatttgaga ttctgggaat tgcccaggca
       61 aaaccgcgtg gaaagctcac tgtgaaagct ggcgaaaaat cattcgaagt gatttgcaga
      121 ctggacaatc ctgtggaaat tgagtactat cgtcatggtg gaattctcca ggcagtactg
      181 agaagcttcc tgaaaggaaa ataaaatgaa cgttcacgac tttacgatca agacaatcac
      241 cggagaagaa aaatctctaa aagagtattc gggcaaaagc ctgatgatcg taaatgtcgc
      301 aagcaagtgc gggcttacgc cgcagtatgc tggactcgaa tctctgtata agaaatacaa
      361 agaccgcggg ttcaacatac tgggcctgcc ctgcaaccag tttcttcacc aggagcctgg
      421 aacagaatca gacatacagg cattctgcag catgaactac ggagtcacgt ttgatcttgg
      481 atccaagatt gaagtgaaag gaaaaacccc gcacccgtta tacgcttttc taactggaga
      541 cggcgccaaa tttccaggga agatttcctg gaattttgaa aagttcctgg ttggcaagaa
      601 cggagaagtt ctcgcaagat tcagcccgca gactgtgccc gaagatcctt ctattgtaag
      661 cgcagtagaa tccagtcttt cctgaaatgc gcgtacacaa ttacgctgca gtacctgatc
      721 cacagaagat cgggacggat ctctatagaa tcgtgatccc gcagcctttc tatgagccca
      781 acaatatcta tctgatcacg tcaggagagc ccactctcat tgatacgggc tacattgaaa
      841 acctgggcct gctccaacga tcacttcgtt ccattggaca ttctctttct aaaatcaagc
      901 acatcatcta cacccatgat cacattgatc atatgagcgc cgcactcagc attcgcttct
      961 acacggacgc gcggacttac gctatggctg gaattgagcc tcatgtagcc aactacgcat
     1021 ccaatcttct cctgatgaag agagcagaag agcgcctgac gtacaaagcg caccgtgatc
     1081 cttctgtgcg aaaagcagaa ctggcacgcg caaggaaagg atgggatgag ttcacagagt
     1141 cttacgctgg tccgggcaaa gtggaaaagg cagttcgaat cgacacaggc ctcatggaag
     1201 gagatgtaat agagtgcggt tcgcgcgaga ttgggtttct atacactccc ggacacaatc
     1261 gctggcacat cacgccttac atcgtagggg aaggaatcta tttcacggga gacctggtcc
     1321 tggacaacgt gtcgagcgtg tatgcggaag tagacggaga tctccagaag tatcacgagt
     1381 ccctggacag gcttttcaaa attccaatcc agagactctt gccggcgcac ggcaatgagc
     1441 cggaagatcc aaaaaagaaa attcgcttgt tgcagaagac tcttgcattg ctcgaaagag
     1501 gcgtcattcg ctgtttaaag gaaaagccgc atgatctggc agaactagta tcatctgcga
     1561 tgggagaaag agtgcgtaaa agtggttact atttcaccgc tctgggagtc atgcattcca
     1621 tcatcaagaa actggcagtc cagggtcaga tcgtggttca tgagatagac ccgccttacg
     1681 aacagtattc actcaaagag acttgagata ttctagaagt gccagtctgt ctgcttcgct
     1741 gatagacgtg ccgtattcat gtcctttgtt tgaattccca cgaagggaag tatcaaataa
     1801 aaaagagccc tgatcagaaa caaatccggc tttctcggga tcatactgat tcgagccaac
     1861 cttgaaggtt gcaggcctct tgtccgccgg aagaagaaga tcgtataacg ttgggacaga
     1921 accgttgtga agaaatggtg cggtggccca gacgccgttg agcggacgcg ctttataggt
     1981 tagaagggag cgaaccgggt tcttcgcatc gcgttcgtat tgaccgtggc gctccgtggt
     2041 aaaccagcca acattggtga tgctggactt tacaatttta tccggctgtt gcgccagaag
     2101 tccttctacg acatgacgaa gcatgtccag cacagaagat ctttctccaa aacgagtcac
     2161 tccgccaacg acgtgcacga atcgaccttt cagtattcca gtgtctgctt ctgcgttcac
     2221 tgcattctgc gcggtcaatg gatctgtacc cagaagctcg atggaagtca tctccgcggt
     2281 gatctttctt ttagggtcat ccctgcgaat gtctgcgtgg cagctcatgc atgtactcgc
     2341 gaagatctct cggccgcgcg cgatttttgc tttgtccaat gacggaaaac ttgccggcca
     2401 ctgcggagac tgtagctgcg ccagtctgga ttcaatctgc atcaggttgg tcatctggat
     2461 ggatgaagta tagcccggtc gaagagagct caggtccaca gttccaaata cgcctaccac
     2521 ctgccctacg ttacgagcaa gcccacctgc gccctggtta gaagcacttc caatccattg
     2581 cacataatcg tgccttggcg catcccatag ataaggaaaa ctcacgggcg catttgcgcg
     2641 gactctatct tttgaatgag ccatccacaa cactcgattg taaattctac cgaatgcgtc
     2701 cagccgcgcc ggaccataag gcgaatctgg atgatttgtg cgattgtact cgcgcctttc
     2761 gtcagccgcc tgcgctaaat actttttgag aacagccacc gcatccggtt ttgtattttc
     2821 gcgacccaga gtgcgctctg ccaggcgcga aaatttctcg ggactcgcaa gagtagtatc
     2881 cagagcagat gaaagatcgt tcaggaatcc ttccatgttg gcaaggctgg atccaccatc
     2941 gattcgaatt ctcttttctg cgtacgtaat ctcaagtgta tgacaggcag cgcatgtaaa
     3001 gccgattgaa tttccacgct tcacaaaacc aatgggatag tcccctggtt tgaccggttc
     3061 gtcgtagaca aagccgtacg cgcgaaggct ttcaggagcg gaaaacaaag catctgaatc
     3121 cgcccgctct acgcgcgaaa agaaatcata tcgcataaac tcggaaccct gatcaattct
     3181 gtaaaaccag gaccgatcgc tctgtttcca accctgatcg agatactgga cagattgtgc
     3241 gaaagaggca gaagtgcatg agagaaacac ggccagtgcg atacgcatgt ctgagtcatg
     3301 aagcaacaat cacaaccgtc aagcattcaa atccgtgcaa cgagacgaaa aatggaatga
     3361 ttctcaggga atccaggagc tggaaaaaag attgagcgcg cagttgacca tttcagccta
     3421 cactcatgcc aaagagtttc atcctctctt atgtagagga caagatgatg aaagaccatg
     3481 cgggtcaaaa agatccgggt ccggtcatca caattgcgcg cgattacgga tgccctggcg
     3541 ttccggttgc ggaggcagtc acaaaagctc tcaccaatta ctatgtggaa tggaagctca
     3601 tagacaaaga aatcatcaac cacgcggcac aggaactgag agtctccccg gaagttgcag
     3661 agagaatcgg acacagcggc ccgcgtggat tctttcacga tttcatcagt gcattttcac
     3721 agcagcagga cccgagcgat gctgcagtga aaagaaccat ggcaaacgca atccgcgcgt
     3781 tctctctggc gggcaatgtc gtaattctcg gccgcggagg agtcgttgtg actcgcgaca
     3841 tcaagcagtc tcttcacgta aaattgtatg gtcctataga ctatcgaatt tcgcgcgtga
     3901 aagaaatgga caatctgaaa agtgacaagg acgccctgca gaagattggc gtggtggatc
     3961 gtgaaagagt ctatcttcgt gactatctgg caggagaaga attgcgaccg gatgtttttg
     4021 atatacaatt gaactgcgga actctcaccg agcaggaagt gatagaaacg atcgtatcga
     4081 ttgcgcgcga caagttcaaa ctggagagat gatgagccgc gaggatgcag ccaggttctt
     4141 acagacagaa agaatcgccg tgctcgccac tgtttctccc gacggaatgc cgcagtcagc
     4201 gctcattgga attgctgcga cccctgattt tgaacttgtt tttgatacaa tcaaaacaac
     4261 tcgcaaatat agaaatctgg cgcaagatcc cagggcatcc ctcgtgatag gctgcactga
     4321 atcgggaatc accttacagt acgaaggtgt agcacgtgaa ctcaggggaa ttgaaatgga
     4381 gaagtaccgc cagatttact ttgcacagtg gccggacggt acagccaggt tgagctggga
     4441 cggaatcacg tacttcgcgg tcagaccatc gtggatacgc tattgcaatt acgcagtgga
     4501 gccgcccgtt ctaacggaat ttacatttcc gttttgaacg ggctgtggtt ccagatcctg
     4561 acctgaggag aaatcaggag aacttgtgca cgagaaaacc agacctgagt ttgggttcaa
     4621 accaggtgga cttgggaggc atgatctgtc ctgcgtctgc aatatcaaac agctcttgca
     4681 tggaagttgg atacatgctg aacgcaaccg cccaggcacc gctgtctaca agcttgatga
     4741 gttccccttc tccgcgaatc cctccgacaa aagcaacgcg ttctgaagtg cgtggatctg
     4801 cgattccaag aattggatcg aggagttgat tttgcagaat gctcacgtcg agcctgccaa
     4861 tggcatccgc gcctttgtac gtttcttctt tcgcagtgag cgtataccat tggcggtcca
     4921 ggtacatatg gactgtatgc aaagcaggat tgctttttcc ttcgctcagc tgaaaagact
     4981 tcttgacttt ttcgaagaac gcttcctttg aaagaccgtt cagatctttc actacgcgat
     5041 tgtatgggag aattcttaga tccgcatccg ggaaagtaac tgcaagaaac cattcatggg
     5101 atccgggtcc tgatgcttcg cgacgagctt tcccaacgcg aacggtggag gcgcttctgt
     5161 gatgaccatc tgcgatgtac acacacgaaa gactttcata gagtgacttg agtttgtctt
     5221 gtttttctcc tacggggatg gagtagacca gatggcgcac gccatcttcc ggagttacgt
     5281 cgtaaagtgg cttctctttg tctgccacct catttaggat gcgagcaatc tctggctgct
     5341 cacccgattt gaaagccaga aatacaggct cgcaattcgc gcctgttgtg tctatgtgat
     5401 gaaggcgatc ctcttctttg tcgcgacgag tcagctcatg ctttttgatt ttgtcttgaa
     5461 ggtagtcgtc tatatggttg agggcagcta gacccgtctg attgcggccg tctttcacga
     5521 gacgatacac atacaggcac ggaccgcttt cgcgcagaag tcttccatca gaaagcatgg
     5581 tttccaggtt cttcttcgca agctgataca ctgcatccgt atgaggatcc gcactatcag
     5641 gtagttcaat ttcagatcga atgatatgaa gaaaagaaga aggcttaccg tgagccatct
     5701 cacgggcctc cgcagtgttc atcacatcgt agggaagaca gaccacctcc gccgcaagat
     5761 cagatctcgg gcggacagca gcaaaagggg aaagtttcat gagtcgcagg ttcctatcta
     5821 agatttagct ggcgcctgcg aagaaaggac ggatcaggtt tcatcccagt aagcactcgg
     5881 ctggctggcc ggtgtgccct gggtaaggta tgctccgtag gactcgagaa gcagtctgca
     5941 ttcgtcctga aaattcgcaa gctgaccaaa caatacctga attcctttgt cctcaaactt
     6001 gtctgtcatt ttgcgataga acgccgccgc atcttcatgc tctcgcgtgg ccagagcgat
     6061 ggcttctatt tcattgccct ttccgtcact tccggtaatt cggtccagac gacgcatcag
     6121 gatctgcaga gaagtaccgt tgaatttttg aacttcagat gccattttca aattcgggag
     6181 cgcttctcca cctttcacct gggaatagat ttcagagatc atgcggatgt gctcttctac
     6241 atcctctgcg agttgaaaaa agagcttctt gattgcacct tctggcaacg cttccgcctg
     6301 cttgaggtaa aaaccgaata cctctttttc gtgctggatg cacgctgcta cggcctcaag
     6361 gaacgtggtc ttcttaacgg gtgttctagc cattggtaga tggaacggtt cgaagagtat
     6421 tctggcaaat gaaatttcat aggccagaga gcattcaatt acctcaaact gaaaagtttg
     6481 acagaaacag atagaccgtc cagatcaaag gcagattcgc cggtagcttt ccccgcatgc
     6541 gatttttcga ttttcgcaag agtctcgtct tcaatgtaac cggcaaagcg ctctatcgca
     6601 ctggagactt ccggatctgc ttccataacc agagtgattc tgtctgtaac ctcaagaccc
     6661 gccttcttgc gcatctcctg aatgtttcga accagatctc tgacgattcc ctcctggatg
     6721 agttcaggag tcaatccggt atcgaggacg acagtcatgc cgcttccctc tactcctgct
     6781 gtcccttctg cagacactgg ctcaacaaga acgtcctctg gttccagttc tatcttcacg
     6841 ccctgatctt ccagggagag agatgaaccg gctcttacgg cgcgagcaac cgtggatgga
     6901 tccttgatac ttctaaagta ttcctggacg gccttcatgt gctggccaac gcgcttgccc
     6961 agccgcggaa gatttggttt gatacgatag tcgatgagac cctgcgcgtc cgccagaaac
     7021 tcaatgctct taacattcag ttcttctttg agaacgtctt cgtttttctc gatcgcagac
     7081 ttagcgcttg catttgccgc aaaaacgcga atgcgcgaaa gcggctgacg aatcttcatc
     7141 ccggatgctg cgcgtgcagc acggccaagg gacgctgcct gcaaaaccag gtctccttcc
     7201 tctagaattt ttgcgtcaaa gaaagatgcg tcatactcag gccagccagc cagattcgca
     7261 ctgatgggcg cgcttttgga ctgcgatgcc acgagaccac gatagagttc ctctccaagg
     7321 aaaggagcaa aaggcatgat caaacgagca atcgtcacga ggcagcgata caaagtatca
     7381 aatcctgtga gcttctccgc atctctttct cccttccaga accttctgcg attgcggcga
     7441 atgtaccaat tggaaagatc atcggtaaag agttctacaa gccgacccgc tttttgagca
     7501 tcgtattcgt tcatcgaggc ggtgacgtca cgaatgagca cctgaagtct cgacagaatc
     7561 catctatcta tgtccggacg ttctttgaaa ggcagagcgc ccgcaaccgg attcttctgc
     7621 cattcattga tttcgattcc atccacattc gcatagaggg aaaagaaacc ggcgctgttt
     7681 tgcagcatgt tgaaaaatcc gaatacatct ccaattggat cctgatccgt tcctggtttt
     7741 cccatcttgc gcgaattgcc gggaggagcg cccaccagga aatgccagcg aatcgcatct
     7801 gcgccgtgca tttcgaagac ctgccacgga tccacagtgt tgcccttgct tttggacatc
     7861 ttttcgcctt tgccatccag gacatgaccc agacagacta cattcttata ggatgtttca
     7921 tcggaaacca tcgtagagat ggcaagtaga gtgtagaacc agccgcgagt ctgatcgatc
     7981 gcttcgcaga tgtagtcggc gggatagtgg ctct
//
LOCUS       DAOQJL010000115         8012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4881_length_8012_cov_4.608772, whole genome shotgun sequence.
ACCESSION   DAOQJL010000115 DAOQJL010000000
VERSION     DAOQJL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_8012_cov_4.608772"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLG78_06870"
     CDS             complement(<1..971)
                     /locus_tag="PLG78_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE22870.1"
                     /translation="MKAAVLYKDQTHLRIEDVPIPELKPGDVRVNIKCCGVCGSDIHM
                     TIHKQVRLRHYPTILGHEASGVVTEVGSDVKKFKKGDRVVVCSGLGCGKCRQCLKGRH
                     NLCPEINVLGAEMNGAYAEQITIEERHLYHLPDQIPFDQGAILADAVSTPYHALKFVG
                     KIEPGEVVAVYGCGGLGIHAVTLARALGASKVIALDVDEGALDNAGKYKADEMIDLRR
                     VRNSGKTLQELTGGVDLMLDFSGHYSNFEDAMRAMNPGGRMVMAGIGKHPFKLTFPFM
                     VINKQISVLGSLGCDSRAIPELIDLYTSGRLDLSTSITSHHPLEDVN"
     gene            complement(1945..2214)
                     /locus_tag="PLG78_06875"
                     /pseudo
     CDS             complement(1945..2214)
                     /locus_tag="PLG78_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(2388..2720)
                     /locus_tag="PLG78_06880"
     CDS             complement(2388..2720)
                     /locus_tag="PLG78_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22871.1"
                     /translation="MLKSYIVLIALSFAAVGSLAAEGRGWLRGSEVLDSRSDVHIANV
                     SQIQPRVGDEFDVFRIILDANPALRGMRHRYSKLGRVRVVEIVDPHTVRASVIWGLTG
                     DSYLIQTE"
     gene            complement(2768..3187)
                     /locus_tag="PLG78_06885"
     CDS             complement(2768..3187)
                     /locus_tag="PLG78_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22872.1"
                     /translation="MVSVSIGRMRLWSKISGELLVAALFFVLLSPLLGVHVHDNGIVH
                     SHSRSTSHNHSDKTIPQNEIPSLPLKLVFNKSLPLEVAHSLLFFVLFLAMFSSHAIAQ
                     PAVVRSVSVRSIRDGTLFLPSPPRRIAGQRAPPISLT"
     gene            3287..3997
                     /locus_tag="PLG78_06890"
     CDS             3287..3997
                     /locus_tag="PLG78_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22873.1"
                     /translation="MSSQSVLTARNLMRIYEMGEVQVRALDGVDLDLFRGEFLVLLGP
                     SGSGKSTLLNLLGGLDVPTSGELTYREHDLKHAGEAELTRYRREHVGFVFQFYNLIPS
                     LTAKENVELVTQIAINPITAERALELVDLSARADHFPSQMSGGEQQRVAIARAIAKRP
                     DLLLCDEPTGALDIKTGQIVLEAIRRVNEEIGSSTVVITHNASIAGMADRVIQLADGK
                     VASETRNKKKLKPSEIHW"
     gene            3994..6342
                     /locus_tag="PLG78_06895"
     CDS             3994..6342
                     /locus_tag="PLG78_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE22874.1"
                     /translation="MSPLNLKAIREIKGLKSQTITTALVALCGIAVQIGYVSAYVSLN
                     SAKERFYTNSRFADIFATVKRAPESVLTTIRNIPGIQQAETGILYEASLRVAGLEEGA
                     SARIVSIGRHDVLNKIRVSEGRLPEVGSATEACVSEGFAHANHLGPGSTVEALINGKS
                     RQVHIVCIALSPEYIYAIRAGSFMPDDKHYGIFWMTREALTDIAGFGGSFNALAIALS
                     PGTKPPDVLKSVDRILDPYGNPGAYARDKQLSNVFLNNELYQLQVMGYSIPIIFLGVA
                     AFLLHIVIGRLINRQREQIATLKAVGYSNLQVGLHYLSIVTFMTLSGALPAILPGIWL
                     GQWTTEMYKDYMKLPDFHFVFDPMVPLWGILFAIVASFLGALSSLKAVMKLNPAEAMR
                     PPAPPRYEKTFIEKAARLTPFTLMGFRHVALKPFQSILSVAGLSMAGAIIVMGFIWTD
                     MVQFLLTVQFNSIQREDVMVNFQGPRPTNVLGEMRVIPGVIYVEGYRIAPIRLHAGHR
                     TKEMALTGLPEGARLRRILKKDFRPNPIPASGILLNESLAKTLSLHPGDIVTLELLEG
                     DRRQVSVPIAGLAEELLGSGAYMEISKMNALLREGPVISTAALSVDLSLLESVQRELR
                     SMPYILSVDSKLFVIRMFHSLYADMLLVMALMLIGFASVIAIGVVYNTVMVALAERSW
                     ELASLRVLGFTQGEVFQMLLVELTLQIIVAIPFGVLLGYHMNSAMLAGLPEDQYHFSI
                     ILQPYAVASCAGILIASAAVSSFLVWQRIKKLDLVSVLKVRE"
     gene            6349..7254
                     /locus_tag="PLG78_06900"
     CDS             6349..7254
                     /locus_tag="PLG78_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HNE22875.1"
                     /translation="MDIQFKNIPGLLADSIKKKPVWWILGTATAIVVVLALRPRPQPV
                     ETATVRRMTFEDTITEDGVTRVKEKFIILSPYSGNLVRLSRHAGDSVKKGEPVAFVFW
                     DRETAVPSPANGSILKVFREDAGPIEIGAPIVEVGDPSSLEIVLEMLTADAVKVAPGN
                     PIRIRGWGGADPLAAKVRIVEPQAIMKISGLGVEERRVRVLADIISPREKWKTLGDNF
                     RLECEVIHYRQDGALVIHSGALFRDQEGWSVFRVEKGKAHKTKVQVERRGSTQSMIKS
                     GLAEGDEVIVYPGEQIKEGVRISEL"
     gene            complement(7255..>8012)
                     /locus_tag="PLG78_06905"
     CDS             complement(7255..>8012)
                     /locus_tag="PLG78_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22876.1"
                     /translation="QAALGYHRAFESIANIAKLPRTREVYERIFALEKESAHAGEFVA
                     KMAAEGLFAKMATVQMIEEYSGLTAQAESVAQPTMAAHNEFVLAAAQKATSAIEIEYE
                     SMRLFELNQLELRLDRTLVNDAFDILGSAISSYLLVPSEENRAAVEAGYRFVADFYAL
                     DSETLFAIPRFMDHMRFMVEHSNKSPGSKKMTVESMTSDFKGAITVCLKGKAPVVLGP
                     SSRRTAVLWGKTVPLEKFHEAIRHPDRPAELQT"
BASE COUNT         1987 a         2096 c         2032 g         1897 t
ORIGIN      
        1 tgattcacat cttcgagtgg atggtgcgat gtgatggatg tcgacaggtc caggcggccg
       61 ctcgtataaa gatcaatcaa ctccgggata gcgcgcgaat cgcatcccag ggatcccaga
      121 accgatatct gcttgttgat caccataaac ggaaacgtca acttgaacgg gtgcttgccg
      181 attcccgcca tcaccatccg cccgcccggg ttcatagcgc gcatggcgtc ttcgaaattt
      241 gagtaatgac cggaaaaatc gagcatcaga tccacgcctc cagtcaattc ctggagagtc
      301 tttccagaat tgcgcacgcg acgaagatca atcatctcgt ccgctttgta ttttcccgcg
      361 ttgtcaagcg cgccctcatc tacatcaagt gcgatgactt ttgaagcacc gagcgcacgg
      421 gccaacgtga ccgcgtggat tccgagtcct ccgcatccgt agactgcaac cacttctcct
      481 ggttcgatct tgcccacgaa tttgagtgcg tgatatggcg tcgacacggc gtcggcgaga
      541 atcgcaccct gatcaaaagg aatctgatcc ggaagatgat agaggtgccg ttcctcaatg
      601 gtgatctgct ccgcatacgc accgttcatc tccgcgccca gcacgttgat ttcagggcaa
      661 aggttgtgtc tgcctttcaa acactgccga catttgccgc atccaagacc agaacacact
      721 acaacgcggt cccccttctt gaatttcttt acgtctgatc cgacttccgt aaccacacct
      781 gacgcttcgt ggcccaggat cgtcggataa tggcgaaggc gaacctgttt gtgaatggtc
      841 atatgtatgt cagacccaca aacgccgcag catttaatgt tcacacgcac atcaccgggt
      901 ttgagttccg gaatgggaac atcctcaatc ctgagatgag tttgatcttt gtacaaaaca
      961 gctgccttca tttttccctc cgcaaaggtc gtatcgggac ggtgcgcaat gatccgcgaa
     1021 tggtagggac cggcccgaga ctgacgcgaa cctcgcggag tgtcgattgt tttttcgggc
     1081 ccggctctgg ctgcgatccg ttatgcgatc aaggtgctct gaattgagat tgacctcctt
     1141 gccgattctt agagcctaat aatcgcgggg tacggcgatt gacaaaatta ctgatcgcaa
     1201 ttgctgctgt tgtgtcattt tgcgtcactg gttgcacaaa ttcacctggc ggcggattgt
     1261 atttggatcc gacgacagtg agtgagctcc tggtggcgaa cctgagttct cctgcatcct
     1321 actttgtcta cactacgagt cttggctaca ctcctctagt ccttggtggc gttgcggggg
     1381 cagatgcgag gtgcatgagc gattctgcct atccggggag cggtatttat aaagcgttga
     1441 tcgtggacgg tacaactcgc cgagcgtgca gcaccgcaaa ttgcgccgga gggtcggcgg
     1501 agcacatgaa ttgggtcttg cgccccaggg caacttacag gcgcttggta agccggcaaa
     1561 cgattgcgac gaccatggcg tcggcgggaa ttttcgtctt cccgctcaca aactctctgg
     1621 attcgtcggg gttgtattat gcggctggtt ttgccagcac agccgattgg cgaacggccg
     1681 caaatacgtg tacgggatgg tcgagtgcgg ctggccttgg agaattagga gaccaaactc
     1741 aggtgaactc cgcggctatc ttcgcgacga gcgcagtttg caccgcgccc gagaatttaa
     1801 tatgtgtgca acaatgagca cggtttcaca agctgtcaat cttcctaatt cccctctgcc
     1861 agacggcagc tattcacact ctccattcta acggtcctga atccacgggg tcaggtcaga
     1921 ttccaccatg ctcgcaaaca gcgactacaa cttgagcaac cgcaaacgaa gcgcattcac
     1981 gatcaccgac actgaactca agctcattgc gagactcgcg atcattggat cgagaagagg
     2041 ccctccgaat agatagagca gccctgcggc aaccgggatt ccgagcgcat tgtaaacaaa
     2101 agcgaaaaac aaattctgcc tgatattcct cattgttgct ctgctcagtt tgatcgcgcg
     2161 gacaattccc atgaggtcac ctttcacaag agtcacgcct gcgctttcga tggctcgagt
     2221 gtgttctgtg tgatgcgatt tatgttccgt catgtctcac tccaatccca aacctcactc
     2281 cggcaaatga gattcaatcg aatcacattc cttcccagct ggcaacctct atggaggctt
     2341 tttcttcaat gaatgccaga tggacagatt gtccatctgg cttcgcttca ttccgtctga
     2401 atcaagtacg aatcgccggt tagtccccag atcacgctgg ctctgaccgt gtgcggatcc
     2461 acaatttcca ctacgcgcac tcgtccgagt ttgctataac ggtggcgcat acccctgaga
     2521 gcgggattcg catccagaat aatccgaaac acatcaaatt catctcctac gcgcggttga
     2581 atttgtgaaa catttgcgat gtgaacatcc gatcgcgaat ccaggacttc gcttcctcga
     2641 agccatcctc tgccttccgc cgccagtgag cccactgcgg caaacgatag tgcaatcaat
     2701 actatataac tttttaacat aattccctcc attttcattc ggccaacaga atgggccgaa
     2761 aaccagatca ggtgagggag atgggaggtg cgcgctggcc tgcgattctg cgcggaggtg
     2821 atggtaaaaa caatgttcca tcacgaatgg acctgacaga aaccgatctt acaacggccg
     2881 gttgcgcgat ggcatgcgaa gaaaacatgg ccaggaaaag aacaaagaac agcaaagaat
     2941 gcgcgacttc cagaggcagg cttttgttga aaacaagctt caaaggtagc gatgggattt
     3001 cattctgcgg aatcgttttg tcagaatgat tgtgactggt agaccggcta tggctatgca
     3061 cgatcccgtt gtcgtgaaca tgcacgccca gcagggggct cagcaaaaca aagaacaatg
     3121 ctgcgaccag cagttcgcca gatattttgc tccagagtct catcctacct atacttacgg
     3181 ataccataca aattctggcc attaaaatct atttcgcgca acgcgaaacc gtcctgcatc
     3241 cgcagcaaat cacttgaaac gagcacgcgc ggatgcgaga tggggcatga gcagccagtc
     3301 ggtcctcaca gcgcgcaatc tcatgcgaat ctatgagatg ggagaagtgc aggtacgcgc
     3361 actcgacggt gtggatctgg atttgtttcg cggagaattc ctggtacttc tgggcccgtc
     3421 tggaagcggc aaatcaacgt tactcaattt gctcggcgga ctggatgttc ccacgtctgg
     3481 agaactcacg tatcgcgaac acgatctaaa gcatgcggga gaagcagagc tcacgcgata
     3541 cagacgggaa cacgttggtt tcgtttttca gttctacaac ctgatcccaa gccttaccgc
     3601 aaaggaaaac gtggaacttg taactcaaat tgcgatcaat ccaatcactg cagaacgcgc
     3661 gctcgaactg gtggatcttt ccgcccgcgc agaccacttc ccttctcaaa tgtcaggcgg
     3721 ggaacaacaa agggtggcta tcgcgcgagc cattgcaaaa agaccagact tgcttctgtg
     3781 tgatgaacct acaggcgcgc tcgatattaa gacagggcaa atcgttctag aagccattcg
     3841 tcgcgtaaac gaagagattg gctcctccac tgtggtcatc actcacaacg cctccattgc
     3901 aggaatggca gacagggtaa ttcagctggc cgacggaaaa gttgcctccg aaactcgaaa
     3961 caagaaaaaa cttaaaccat ccgagattca ctggtgagcc ctctcaatct caaagcgatc
     4021 agggaaatca aaggcctcaa aagccagacg atcacaacgg ccctggtagc gttatgcggc
     4081 attgcagtcc agattggcta cgtaagcgcg tacgtttctc ttaacagcgc aaaagagcga
     4141 ttctacacaa attctcgttt cgcggacatt tttgcgaccg taaaaagagc tccggagtct
     4201 gtactcacta ccatccgaaa tattccgggc atccaacagg cggaaactgg aattctatac
     4261 gaagcgagcc tgcgagttgc tggtctggag gaaggtgcgt cggcgcgaat tgtttcgatt
     4321 ggtcgtcacg atgttctgaa taagatccgt gtgagcgaag gaagactgcc ggaagtggga
     4381 agtgccacag aggcttgcgt gagtgagggt ttcgcacatg caaatcatct gggccctggc
     4441 tcaactgtag aggccctcat caacggcaaa tcgaggcagg tccacattgt gtgcattgct
     4501 ctgtcaccgg aatatattta cgcgattcga gctggcagct tcatgcccga tgacaagcac
     4561 tacggcatct tctggatgac gcgcgaagca ctcacggaca ttgcagggtt cggcggttca
     4621 ttcaacgcac tggccatagc tctgagtcct ggaacaaagc cgcctgacgt tctaaaatct
     4681 gtagatagaa ttctagatcc ctatggaaat ccgggagcgt atgcccgaga caaacaacta
     4741 tctaacgttt ttctaaacaa cgaactctac cagctgcagg ttatgggcta cagcattcca
     4801 atcatctttc tgggagtggc ggcattctta ctccacattg tgattggaag attgatcaac
     4861 cgacaaaggg aacaaattgc gactctaaaa gcagtgggat attcaaatct tcaggtaggt
     4921 ctacactatt tgtccatagt cacatttatg acgttaagcg gcgctcttcc tgccattctc
     4981 ccgggtatct ggctggggca atggaccacg gaaatgtaca aagactacat gaagctcccg
     5041 gattttcatt ttgtatttga tccaatggtt ccactgtggg gaattctttt cgcgattgtt
     5101 gcttccttcc tgggtgcgct ctccagcctg aaggctgtga tgaaactcaa cccggcagaa
     5161 gcaatgcgac ctccagctcc accacgctat gaaaagactt tcatcgaaaa ggcggcaagg
     5221 ctgactccct ttaccctcat gggattcaga cacgtggccc tcaagccctt tcagagcatt
     5281 ctttctgtcg ccggattgag catggccggt gccatcatag tcatgggttt catctggacg
     5341 gacatggtcc aatttctact cacggtgcaa ttcaacagca tccagcgcga ggatgtcatg
     5401 gtgaacttcc agggaccaag gccgactaac gttcttggag aaatgcgcgt cataccgggt
     5461 gttatctatg tagagggtta cagaattgcg cccattcgtc tgcacgcagg acatcgaacc
     5521 aaagaaatgg cactcacagg tcttcctgag ggtgccaggc ttcgcagaat cctaaagaaa
     5581 gactttcgac ccaatccgat tcccgcctca ggcatattac tcaacgaatc tctggcaaaa
     5641 acgctttctt tacaccccgg agacattgta acgctggaac tcctggaagg cgatcgccga
     5701 caggtatccg ttccaatcgc cgggttggcg gaagagctgc tcggatcagg tgcctatatg
     5761 gagatctcca aaatgaacgc acttctccgc gagggccctg tgatttctac tgcagctctt
     5821 tctgtggatc tgagtttgct cgaatcagta cagagggagc tcagatccat gccatacatt
     5881 ctttctgtgg attctaaact attcgtaata cgtatgttcc acagtctcta tgctgacatg
     5941 cttctggtca tggctttgat gctgattggt tttgcgagtg tcattgcgat cggagtagtc
     6001 tacaatactg tgatggtggc tctcgcggag agatcctggg aactggccag ccttcgcgta
     6061 ctcggattta cgcaaggaga agtattccag atgctcctcg tagagctgac tcttcagatc
     6121 atcgttgcca ttccattcgg agtgctgttg ggttaccaca tgaactccgc gatgctagca
     6181 ggccttccag aagatcaata tcacttctcg atcattcttc aaccgtatgc tgttgcaagc
     6241 tgcgccggta ttctgattgc atctgcggca gtatcctctt ttctcgtctg gcaaagaatc
     6301 aaaaagctgg acctggtttc cgttctaaaa gtgcgcgagt aagtgatcat ggacattcaa
     6361 ttcaaaaaca tcccgggcct gctggccgat tccatcaaga agaagccagt gtggtggatt
     6421 ctcgggacag ctactgcaat cgtcgtggtg ctcgcactgc gaccccgacc acaacctgtc
     6481 gaaacagcaa ctgttcgtcg tatgacgttc gaagacacaa tcacagaaga tggcgttacg
     6541 cgtgtaaaag aaaagttcat catcctttcg ccttatagcg gaaaccttgt gcgcctttcg
     6601 cgacacgcag gagattcagt gaaaaaaggg gagcctgtag cgttcgtatt ctgggatcgc
     6661 gaaacggccg ttccatcgcc tgcaaacggc tccatattga aagtatttcg cgaggacgca
     6721 ggcccaatcg aaatcggagc tcccatcgta gaggtgggtg atccatcttc acttgaaatc
     6781 gttctggaaa tgctgactgc agacgcggtg aaagtggctc ccggaaatcc aattcgcatt
     6841 cgaggatggg gtggtgcgga tcccctggca gcgaaagtga gaattgtaga accgcaagca
     6901 atcatgaaga tatccggcct gggagtggaa gaaagacgcg tgcgcgtact tgcagacatc
     6961 atctcacctc gcgaaaaatg gaaaacgctt ggagacaatt tcagactgga atgcgaagtc
     7021 atccactaca gacaggatgg agcgctcgtc attcacagcg gcgcgttgtt tagagatcag
     7081 gaaggatggt ctgtatttcg cgtagaaaaa ggaaaggcgc acaaaacaaa ggtgcaggtg
     7141 gaacgtcgcg ggagcaccca gtccatgatc aagtccggtc tggcagaggg agatgaagtc
     7201 attgtttacc ccggagagca aatcaaagaa ggcgtgagaa tctccgaact gtaatcaggt
     7261 ctgcaattca gccgggcgat ccggatgacg aattgcctcg tgaaactttt ctaatggtac
     7321 ggtctttccc cacaaaactg ctgttctgcg agacgaagga cccagaacga cgggtgcttt
     7381 tcccttcaag cagactgtga tggccccctt gaagtcagaa gtcatggact ctaccgtcat
     7441 cttcttacta cccggcgatt tattggagtg ctccaccata aaccgcatat ggtccatgaa
     7501 cctgggaatg gcgaataacg tttcagaatc cagtgcgtaa aaatccgcga caaagcgata
     7561 tcccgcttcc acggccgcgc gattttcttc ggacgggacc agcaggtagc tggaaatggc
     7621 ggatccgagg atgtcaaatg catcatttac aagagttcta tccagacgaa gctccagctg
     7681 attgagttca aacaggcgca tggattcata ttcgatttca atggcactcg tggccttttg
     7741 tgcggccgcc aggacaaact cattgtgtgc ggccatagta ggctgagcca cagactcagc
     7801 ctgagccgtg agaccggagt attcttctat catctgtact gtagccatct tcgcaaacaa
     7861 gccttccgcc gccatcttcg cgacgaattc acccgcatgt gcagactctt tttcaagcgc
     7921 aaagatacgt tcatacactt cgcgcgtccg gggcaacttt gcaatgttcg caatgctttc
     7981 aaaagcgcgg tggtatccaa gtgctgcctg cg
//
LOCUS       DAOQJL010000116         8006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4887_length_8006_cov_4.603949, whole genome shotgun sequence.
ACCESSION   DAOQJL010000116 DAOQJL010000000
VERSION     DAOQJL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_8006_cov_4.603949"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..425
                     /locus_tag="PLG78_06910"
     CDS             99..425
                     /locus_tag="PLG78_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE22877.1"
                     /translation="MNISTRKKGNHLIVALAGELNLYNVGEVKNQITTTIGSDTSSLV
                     MDFKDLKYIDSSGIGLMAQLKKKYSAGSGKFALMGVSKEVMDVLRITALDSFFTFISS
                     EDKLEG"
     gene            complement(447..1415)
                     /locus_tag="PLG78_06915"
     CDS             complement(447..1415)
                     /locus_tag="PLG78_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22878.1"
                     /translation="MKRALVASVLLASATLHADVLRLRSGESVEGFYQSEESGIISFV
                     KITGEKMTIPSEEVENLEFSQSGIPICYTLKTSAERRCEALLGSVSSDGFVIAEGKSK
                     RSVRKIPLDDMLSYQLRKVADYQKIGPYLALDKKAVLVTNTGEKLAGRVLETVPDGVI
                     VRDAGGSNHTVQESSIASADVNMVTVPFHWFDLNNVIVGRPQMQSGRVWMGRSLMGGF
                     IGMWTGFVYEYYLASSMTKKAKGDPTVLLFNNQSYFKEFQGHQNGQRYFAAGALAIYA
                     FHWFDLYRHRDSVTSIDVQIAEAPLPDLSVRQRDPWSLALVWSYRY"
     gene            complement(1412..2947)
                     /locus_tag="PLG78_06920"
     CDS             complement(1412..2947)
                     /locus_tag="PLG78_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22879.1"
                     /translation="MSQSRILFPLILGLALLFALITQSIISVTMLELRVQIASERILN
                     FELSSRMLKTRFRQMLSGNENLKSEIKLNVLESSVLNFDKFELDGTDSWLNRFGVVVI
                     NGVRRLNLKPPIRIQEDREFMTKLQYAFYMERNRRYDVAFKQYEDMLNSGDNSQNDTR
                     GFLKLHSGYSAALTGRKDVAVSRLEQVIQEYAGTHYAETATVILDILREQGRAAEKIE
                     ASGMSDRERAAAYFRAGNFGEALTRFKKLEDLSLDEKYMLSRSYEETGNVKEAISGYI
                     DVVNRGGSSDPAVKSNRRLLLIGNFYNGGKEVREFSQQNATRLGDQEVLRNVEEGKEL
                     QLKPVIVEKILKSNDQKQSALVEEIKKELDEGTDGSSRVAVARPALPDETGALRVSVP
                     DRPDPGAITLERPSPPAIFVAQARLKVRFKDGRVVSGTRIAINKDVASVYSGDFEISV
                     PISNMEKIDAADGASALGVTPAAGNGFDTSTIRVEKEEMKFKRGGADESLPVSAIKEI
                     KAK"
     gene            complement(2944..4446)
                     /locus_tag="PLG78_06925"
     CDS             complement(2944..4446)
                     /locus_tag="PLG78_06925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22880.1"
                     /translation="MKKESKSRRSLRVQIGLLYTLLAFVNIIFFSVMIFSNQRDLLIL
                     NFRYQADNLVKSVQERLEKESVPLEPNGAFEDFKAALKANEITDFTIYDDKGVIWHSA
                     PDTSQRGKQVPDELKRKSIELSADLTLYRSSYLKTINEQDFTVSFLLPLRGEGDKRLY
                     LSTILSLQAIKQALRGIYIQIAIAVVWGIVFHVLFAIFLFNLIFRRVEVLTQASNEMA
                     AGNLKSRAQWKTDRNDELDNLGHAFNSMAANIEEKVETISKLNEEIQTELRIGKEVQK
                     QFIRGTDIIPKYKGQIYYRPMREVSGDIYGMFAFPKGFNGFFFADASGHGVSAALITT
                     ITIQNLENAARKFMNPRKIVSTLNNSLAARFESSYFATGTFFLFHPNGETLITNAGHL
                     PILCLRPSTGDILEIAKMGPPMGLMEDFEYPVKRLNTQPGDRLLVYSDGLMETNNVEK
                     EQFSLARIKELFLQHSKNSTEEFLADLVQQFETWAAEYRDDVSAIVLEVP"
     gene            complement(4504..5553)
                     /locus_tag="PLG78_06930"
     CDS             complement(4504..5553)
                     /locus_tag="PLG78_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22881.1"
                     /translation="MRVLSSIILFALSATVAVAEDGKCVAGDCTKGSGTAIWGADVYT
                     GSFQNGKPGGPGTFKLADGRTFSGNFTNGEATGQFQVNYPDGSIYNGELVGGRRKGKG
                     TLKSMRGIYRGEFDQDKIQGQGTFDFASGEVYKGEFKDGQMHGQGTMKFRDGTLYKGE
                     FKDGKMAGPAHVEYPDGRIYDGEFTGGHMQGTGKLALKDGAVYEGDFANGQMHGQGTI
                     RFANGDKYSGEMDHGKIQGRGKLEYHEGAVYEGEFKSGLPHGEGELSSPRGIKYKGEF
                     KNGQMEGKGEIFYKSGSRYVGQVKDGKKEGEGEWTLSNGTVLKGRFKGDSYAGSAETQ
                     ESDPSKPPEKGQKGE"
     gene            complement(5633..6031)
                     /locus_tag="PLG78_06935"
     CDS             complement(5633..6031)
                     /locus_tag="PLG78_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22882.1"
                     /translation="MKGNNILLRLTIFIVPLLSSCASYNIALYDTKIPVYFTEGDTPD
                     QAGRHFKFERYLTWILFDTVKMQDLNLDEAIKAEVPTAKKIVNLRVSSSESGVDSVVR
                     FFGTLIQYLLVADRSLVSKRTVIVEGDYYE"
     gene            complement(6018..6440)
                     /locus_tag="PLG78_06940"
     CDS             complement(6018..6440)
                     /locus_tag="PLG78_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22883.1"
                     /translation="MRVVCILLLSLLFLAGCAETQYKVNSRDLVVRLNGFQKSEGFTK
                     KREFEIKFRRYYCLWGLIRLTDRTLDDLLKEETRPGDFVGNLQINEKYDFADGLVDTI
                     LYGLLRPYSVVVRGEIYMRSAVTEPAETKKVEGKDEGK"
     gene            6647..7429
                     /locus_tag="PLG78_06945"
     CDS             6647..7429
                     /locus_tag="PLG78_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE22884.1"
                     /translation="MRVDWSNPNLLSVLAALGSVFLILMIRYFVMAGGAYALFWKILP
                     DRLRARRIQKDLPRKGHIKSEILYSISTSVIFSFVGLFLGYGKYKGWFQIYTDFDSRG
                     WLYFFGSLFIALLLHDAYFYFAHRLMHTRFLFKQLHRVHHESTNPTPWAAFSFHPYEA
                     VLEALVLPLILVFVPMHVYAVLLFMFSMTALNVLGHLGYELYPNGFTQSKWTFWNNTS
                     THHNMHHRYVHCNYGLYFNWWDRAFKTNHARYHERYEEITAR"
     gene            complement(7448..>8006)
                     /locus_tag="PLG78_06950"
     CDS             complement(7448..>8006)
                     /locus_tag="PLG78_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22885.1"
                     /translation="SYQAFSYELKCAGNEFCSLTNAFEVGYSLTQAVSVSAMLTAVAF
                     ACTSGWFRKTLFVYCILNAAIYVVVTILGVTQPNKTLLSFEVLMLFALPGILLVILVS
                     GIQYARKRKPLDGSLFGAAVLLVVVNAAYFTYYGAGITAALYKKGQGFYFSENDVLHV
                     GMILWLVYVVKVVGKHLRDFDARSV"
BASE COUNT         1941 a         2161 c         1818 g         2086 t
ORIGIN      
        1 ccgccgttct gaggtccatt ctactccata cagatagagc aggcacctcc cgtagaaaat
       61 gattgacact gaccttcctg gaaaggaact ttcgacagat gaacatttcc acacggaaga
      121 aaggcaatca tttgatcgtc gctctggcag gagagctgaa cctgtacaac gtgggcgaag
      181 tgaaaaacca gatcaccaca accatcggct cggatacgag ttctctggtc atggatttca
      241 aagatctcaa atacattgat tcttctggaa ttggcctcat ggctcagctc aagaagaaat
      301 acagcgcagg cagcgggaaa ttcgcactta tgggagtgag caaggaagtc atggatgttt
      361 tgcgcatcac tgctcttgac agcttcttta cattcatcag ctcagaagac aaactggaag
      421 gctgatcttc gccttcgcgc gaccaatcaa tacctgtagg accagaccag agccagactc
      481 caggggtctc tctgtctcac ggacaaatca gggagcggag cttccgcaat ctgtacatct
      541 atactggtaa cagaatctct gtgtctgtat aaatcaaacc agtgaaacgc gtagatggcc
      601 agtgcgccgg ctgcaaagta tctctgtcca ttctgatggc cctgaaactc tttgaaataa
      661 gactgattgt tgaagagtag aaccgtggga tcccctttcg ccttcttagt catcgagctg
      721 gccagatagt attcgtacac aaagccagtc cacattccaa tgaaaccgcc catcaacgat
      781 cgccccatcc agacccggcc actttgcatt tgtggtctgc ccacaattac gttgttcaaa
      841 tcaaaccagt gaaaaggtac tgtgaccatg tttacatctg cgcttgcaat cgaactctct
      901 tgcacagtat gattggatcc acctgcatct cgcacgatga ctccgtccgg aaccgtctct
      961 aaaactcttc cggctaactt ctcgcccgtg ttcgttacca gcactgcctt cttgtcgagt
     1021 gccagatacg gacctatttt ctggtagtcc gctaccttgc gaagctgata ggaaagcatg
     1081 tcatccagag gaatctttcg cacgcttcgt ttggactttc cttctgcgat gacaaagccg
     1141 tcagaagaaa cactgccgag caaagcttcg catcttcttt cagcgctcgt ttttagagtg
     1201 taacagatgg gaatgcccga ttgcgaaaac tcgagattct ccacttcttc agatggaatg
     1261 gtcatcttct ccccggtgat cttcacaaag ctaatgattc cggattcttc tgattgataa
     1321 aagccttcta cggattcacc gctacgaaga cgcaaaacgt ccgcatgcaa cgtcgcagat
     1381 gcaagcaaaa cagatgcgac aagcgcacgt ttcattttgc cttaatttcc ttgatcgcag
     1441 aaactggaag ggattcgtcc gcgcctccgc gcttgaattt catttcttct ttctccacgc
     1501 gaatcgtaga agtatcaaaa ccatttccgg cggcaggggt cactccgagc gcagacgctc
     1561 cgtcagcagc atcaatcttt tccatgttcg aaatcgggac ggagatttca aaatcaccgg
     1621 aatacacact ggccacatcc ttgttgatgg caatgcgcgt accgctgacc acgcgaccat
     1681 ctttgaatcg cactttcagt cttgcctgtg caacaaaaat ggctggagga gaaggtcgct
     1741 ccagtgtaat ggcgcctgga tcaggacggt ccggcacgga cacgcgaaga gcgcctgttt
     1801 catccggcaa tgcaggacga gccaccgcca cgcgagagga gccgtctgtg ccctcatcca
     1861 gttctttttt gatctcttcc accagcgcgc tctgcttctg gtcattagac ttcagaatct
     1921 tctctacgat tacaggcttg agctgcaatt ccttgccctc ttccacgttt cgcagcactt
     1981 cctggtctcc cagacgcgtg gcgttttgct gcgaaaattc gcgcacttcc tttccaccgt
     2041 tgtaaaaatt gccaatcaga agcaatcgcc gattggactt cactgcagga tcactgcttc
     2101 caccgcgatt gaccacgtcg atgtatccgc tgattgcttc ttttacattt ccagtctctt
     2161 cataggagcg cgaaagcatg tatttctcat ccagactgag atcttccagc ttcttgaatc
     2221 gagtcagagc ctctccaaag tttccagcgc gaaagtaagc cgcggccctc tcgcgatcag
     2281 acattccgga tgcttcaatc ttttccgccg cgcgcccttg ctcgcgaaga atatccagaa
     2341 tcactgtggc tgtttccgca taatgagttc cggcatattc ctgaatcacc tgttcgagtc
     2401 ttgaaaccgc cacgtctttt ctgccggtga gcgccgctga ataaccggaa tgaagcttga
     2461 gaaatccgcg agtgtcgttc tggctattgt ccccggaatt cagcatgtct tcgtattgtt
     2521 tgaaggctac atcgtatcta cgatttcgct ccatataaaa cgcatactgg agctttgtca
     2581 tgaactcgcg gtcttcctga atgcgaatgg gcggcttcaa attcaatcgc cgcacgccgt
     2641 taatgacgac tactccaaat cgattcaacc agctgtccgt tccatctagc tcaaatttgt
     2701 caaagttcag aacactggat tcaagcacgt tgagtttgat ttcggacttc aggttctcat
     2761 tcccggatag catctgtcga aagcgcgtct tgagcattcg agaagaaagc tcgaaattga
     2821 gaattcgctc gcttgcaatc tgcacgcgca gctcgagcat cgttacgctg atgatggact
     2881 gggtaatgag cgcaaacaat agcgcaagtc ccaggatcag cggaaacaga atgcggctct
     2941 ggctcacggc acctccagca caatggcgga gacatcatcc cggtattcag cagcccatgt
     3001 ttcaaactgt tgaaccagat ctgcgagaaa ttcctctgtt gaattcttag aatgctgcaa
     3061 aaacagttcc ttgattcgag caagggaaaa ctgctctttc tccacgttgt ttgtttccat
     3121 cagaccgtct gagtacacaa gcaggcgatc cccgggctga gtgttcaagc gcttcacagg
     3181 atattcaaaa tcttccataa gacccatggg tgggcccatc ttcgcaatct ccagtatatc
     3241 cccggtagaa ggacgcagac agagaatcgg aagatgtcct gcatttgtga tcagtgtttc
     3301 tccattggga tgaaacagga aaaaagttcc agtggcaaaa tacgaagact caaagcgcgc
     3361 agccagactg ttgttgagag tggaaacaat cttgcgcggg ttcatgaatt ttcgcgcagc
     3421 attctccagg ttttgaatgg taatggtagt aatgagagcc gcggatactc cgtgacccga
     3481 tgcatccgca aaaaagaatc cgttaaaccc tttggggaac gcaaacattc cgtagatatc
     3541 gccgctcact tcgcgcatag ggcgatagta aatctggcct ttgtattttg gaatgatgtc
     3601 tgtcccgcga atgaactgct tttgcacttc cttaccaatg cgaagctcag tctggatttc
     3661 ttcattgagc ttggagatcg tctccacctt ttcctcaatg ttggcggcca tagagttgaa
     3721 ggcgtggccc aggttgtcta actcatcgtt tcgatccgtc ttccattgag cgcgcgactt
     3781 aagatttccg gcggccatct cgttggatgc ctgagtgagc acttccactc tgcggaaaat
     3841 caaattgaat agaaagatag cgaataacac atggaagacg atgccccata ctactgcgat
     3901 tgcaatctga atgtaaattc cgcgaagagc ctgcttgatt gcctgaagac tgaggatggt
     3961 ggaaagatag agtctcttat ctccttcgcc gcggagcgga agcaagaacg acacagtgaa
     4021 atcctgttcg ttgattgtct tgagataact ggaacgatac agagtcagat ccgcagaaag
     4081 ctcaatggac tttcgcttta attcatctgg aacctgtttg cctctctgcg atgtatccgg
     4141 cgcactgtgc cagatgactc ccttgtcgtc gtagatggtg aagtctgtaa tttcgtttgc
     4201 tttgagcgcg gctttgaagt cctcaaacgc accgttcggt tccagtggaa cgctttcttt
     4261 ttcgagtctc tcctgaactg atttcaccag gttgtccgcc tggtagcgaa agttcagaat
     4321 cagcaagtct ctctggttcg agaagatcat cacggagaag aagatgatgt tcacgaacgc
     4381 aagaagcgta tacaatagac cgatctggac ccggagagat ctgcgacttt tggactcttt
     4441 tttcatggcc ttatcttcta agatcggcct ttcaggccgt tttccggtgc accggcggcc
     4501 taactactca cctttttggc ccttttccgg gggtttagat gggtccgatt cctgcgtctc
     4561 ggcacttcca gcgtagctgt ctcccttgaa tctgcccttg agaacagtgc cgttgctcag
     4621 cgtccattcc ccttctcctt ccttcttccc gtcctttacc tggcctacgt atctcgatcc
     4681 actcttatag aagatttctc ccttaccttc catctggccg tttttgaatt ctcccttgta
     4741 tttgattcca cggggagaag agagttcgcc ttcgccgtga ggcagaccgg acttgaactc
     4801 cccctcgtaa actgctccct catgatattc cagtttccct cgcccctgga tcttaccgtg
     4861 atccatttca ccggagtact tatctccgtt tgcgaagcga atggtgccct ggccgtgcat
     4921 ctggccgttt gcgaaatctc cctcatagac agctccgtct ttcaaggcga gcttgcccgt
     4981 gccctgcata tgtccgccag tgaattctcc gtcatagatc ctaccatccg gatactccac
     5041 gtgcgccggg ccggccatct ttccgtcctt gaattcccct ttgtatagag tgccgtcgcg
     5101 gaatttcatg gttccctggc cgtgcatctg gccgtcctta aattcccctt tgtacacttc
     5161 cccgcttgcg aaatcaaacg tgccctggcc ctggattttg tcctggtcaa actctccgcg
     5221 gtagatgccc cgcatgcttt tgagagtgcc ctttcctttt ctgcggccgc cgacgagttc
     5281 gccattgtag atggaaccgt ccgggtagtt tacctgaaac tggcccgtgg cttccccgtt
     5341 tgtgaaatta ccagaaaagg ttcttccatc cgcgagtttg aatgtacccg gacctccggg
     5401 cttgccgttc tgaaacgatc cggtatagac gtccgcgccc cagatggcgg ttccactgcc
     5461 tttcgtgcaa tcccctgcca cacactttcc gtcctcagcc acggctaccg tggcagacag
     5521 ggcaaacaaa atgatggagc tcagcactct cacaattcaa taagacgatc gggcgcgcac
     5581 tctgtccaca cgaaagcgat aggattctcc cgactgcggt gacacgaatg catcattcgt
     5641 aatagtcacc ttccacgatt acagtccgct tcgagacaag agaacgatca gccactagca
     5701 gatattgaat cagagtccca aaaaagcgga cgacgctatc cactccggat tctgaagaac
     5761 tcacgcgcag gttcacgatt ttttttgccg tggggacttc cgctttgatg gcttcgtcta
     5821 gatttaagtc ttgcatcttg actgtgtcga acaggatcca cgtgagatat ctctcaaact
     5881 taaagtggcg cccggcttga tctggcgtgt caccctcggt gaaatagaca gggattttgg
     5941 tgtcgtagag tgcaatattg taagacgcac aactcgaaag caacggcacg ataaaaatcg
     6001 tcagcctcag aagtatgtta tttcccttca tccttgccct ccactttttt tgtttcagcc
     6061 ggctctgtga cggcgctccg catgtagatc tctccgcgca caactacgga gtacggccgc
     6121 agcagcccgt aaagaattgt atccaccagt ccatccgcaa aatcgtattt ttcattgatc
     6181 tgaaggtttc cgacaaagtc gcctggtcgc gtttcctcct tgagcaaatc atccagcgtt
     6241 cgatctgtga gtcgaatgag accccagagg caatagtagc gtcggaattt gatttcaaac
     6301 tcccgcttct tcgtaaaacc ttcgctcttt tgaaaaccat tgaggcgaac cacgaggtcc
     6361 cgactgttga ccttgtactg ggtttcagcg caaccggcca ggaacaaaag gctgagtaat
     6421 agtatacaaa cgactctcac aaaattcctc cgtccgcgca aaatagcatg catgtatcaa
     6481 gaaccccggt caaatagaaa ttagccgcct ggcaatttat tcgagcttat gttgaaaatg
     6541 ggaaccagat tcaaccataa caattgatgt ccccttccac ggctgccttt gagcttgcag
     6601 gcaagaagga tgaaaccgac tctggatcca gaaaggtcta ataattatgc gtgtagactg
     6661 gtccaatccg aatcttttat ccgtgctggc tgctcttgga tccgtgtttt tgatcctgat
     6721 gattcgctac tttgtcatgg ccggcggggc gtatgctctg ttctggaaaa tacttccaga
     6781 tcgacttcgc gctcgtcgca tccaaaaaga ccttccgcga aaaggacaca tcaaaagcga
     6841 aatactgtac tccatttcta catccgtgat cttttcgttt gtaggactct tcctcgggta
     6901 tggaaaatac aaagggtggt ttcagattta cacagatttc gattccagag gatggctgta
     6961 tttcttcgga agtctgttca ttgcccttct attgcatgac gcctattttt atttcgcgca
     7021 tcgtctcatg catactcgat ttctattcaa gcaactccat cgagtacatc atgaatctac
     7081 gaatccaaca ccctgggccg ctttttcttt tcatccatac gaggcagtcc tcgaagctct
     7141 ggttctaccc ttgatcctgg tctttgttcc catgcatgtt tatgcagttc ttctattcat
     7201 gttctccatg actgctctca acgtactggg tcatcttgga tatgaacttt atccgaacgg
     7261 attcacccaa agcaagtgga cgttctggaa caacacaagc actcaccaca acatgcatca
     7321 tcgttacgtg cactgcaatt atggactgta cttcaactgg tgggatcgcg cattcaaaac
     7381 caatcacgca cgctatcatg aaagatatga agagatcacc gcccgatagt gcggggccga
     7441 agcccgatca aacagagcgg gcgtcaaaat ctctcagatg ttttccgacg actttcacca
     7501 cgtacacaag ccagagaatc atcccaacat gaagcacgtc attttctgag aaatagaatc
     7561 cctgcccttt cttgtacaga gccgccgtga tccctgctcc gtagtaagtg aagtaggcag
     7621 cgttcaccac gacaagcaag acagcggcgc caaagagaga gccgtctaga ggcttgcgtt
     7681 tgcgcgcata ctggatgcca gaaaccagaa tcacaagcag gattccaggc aaagcaaaaa
     7741 gcatcagaac ttcaaaagaa agaagagtct tgttgggctg cgtgacgccc aggatcgtaa
     7801 cgaccacata aattgcggca ttcaatatgc aatagacaaa gagagtcttg cggaaccatc
     7861 cgcttgtgca ggcaaaggcc accgccgtga gcatggcact gacactgacc gcctgggtca
     7921 ggctgtatcc tacttcaaac gcattcgtaa gcgaacagaa ttcatttcca gcacatttca
     7981 actcgtagct gaatgcctgg taactg
//
LOCUS       DAOQJL010000117         7957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4935_length_7957_cov_4.405340, whole genome shotgun sequence.
ACCESSION   DAOQJL010000117 DAOQJL010000000
VERSION     DAOQJL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_7957_cov_4.405340"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /locus_tag="PLG78_06955"
     CDS             complement(<1..2283)
                     /locus_tag="PLG78_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNE22886.1"
                     /translation="MSANLTDKQKKIILGTEKTLFISLFAGAILGGLILGLAVREVAR
                     ARDLILVSAYRPSTPTRLYDKNGKVFAELFRQRQELVQLQDIPPHVIQAFLSVEDDNF
                     FNHIGLDVSGILRAAVKNAISGKVRQGGSTLTQQVAKQIYLNAEGKRNRTIRQKIRET
                     LLAFEIEENYTKYEILEVFFNVIYLGHGCKGIACASRLYFNKKVTDLTLAEAALLARM
                     PKSPVEYSPFKNPDECMRQNKIVLRQMADHGYLPKDQVEAVHKKFWDEYWPRVVVQSP
                     SQNIWADRLDKAPFFTDYVRQILEASEEVGPELLYTGGLKIYTTLDLDHQRIAEEELL
                     KKVAEVNQTSRAYAKAGGKGGVDMSLFGIFSQLSMILPVGSPDVKGLDIRGQFRKAYE
                     EEMIEPTQLLGYSTPAFNEAAAFEEFRKATISYTTNLQVQGAFISIEPRSGYITSMVG
                     GTEFTPQNQFNRALQARRQPGSAFKIFIYGAAFEKRLISTQSAVNDAPFYSIDAVGNN
                     WSPGNYDEGFRGLTPVFAAFALSINTCAIQVYYKVGPDAIIEFAQRLTKITNKDRFHP
                     DPSLALGSSEVTPFEMAKAVAVIGNDGKDVIPFAVRYVTDQSGNTLYNQEENIRKIIK
                     AKTDENRIQIIDPALAYLLRKIMIAVADHGTATLGVRQTAQFTGSLAAKTGTTSSWSD
                     AWIVGFNPDVAAAIWFGFDKSSVTLGAGQAGGHIAAPTMGRFYKRIYDEKHLKPPMFS
                     DRPDGDRPPPGIVTAGCGGEA"
     gene            2331..3161
                     /locus_tag="PLG78_06960"
     CDS             2331..3161
                     /locus_tag="PLG78_06960"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE22887.1"
                     /translation="MQRWEIQEPQAPPLTEGVFRLLVLFSLAFGIEWIAAAGFQRPID
                     SLALSFGPDFQPTQILTHIFLVIPPGFFGGLSLFFLCLMLWGFGGDIERLWGPRHFLN
                     LFLAGMAGSILLGLIAHLSAFPGLSLHGPTGGISAVLLAYAVLWPDRKALFFFVIPMR
                     MRWVILVFAVLIAVSSMVALLQVLGGALGGALFIYYYLRKGNRTYGQSVEDRTMTLSE
                     RWEEFKKKRRLNKKKAEIERRIEMKEEVDRLLEKISKSGMKSLSSKEKALLDRASREL
                     "
     gene            3163..3645
                     /locus_tag="PLG78_06965"
     CDS             3163..3645
                     /locus_tag="PLG78_06965"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNE22888.1"
                     /translation="MRIVWIGSWIVSVALLATVDCKPRTLTEVPMPDYKSFRHVKSGL
                     LLKGDADDLLTGIHHVYANDAAVEALRNRSDFPEDSVLVFDLFEPLDTPEGYKEGAHK
                     FTAVMHKHSSFKSTGGWGYEAFLPDGKRVVSDANKMCFECHKSVESQGYVFTNAGMSE
                     "
     gene            complement(3723..4076)
                     /locus_tag="PLG78_06970"
     CDS             complement(3723..4076)
                     /locus_tag="PLG78_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22889.1"
                     /translation="MNKFIVVLIALFVSGALLSQERNTKCKDELARICPGYGGDGDKL
                     GACLKDPEKSKQLSPECKPRRGGFPAIKASCSTEIQSYCSTATEDGKLYMCIRQQMNK
                     NPSSVGQNCKDQMNK"
     gene            4276..4788
                     /locus_tag="PLG78_06975"
     CDS             4276..4788
                     /locus_tag="PLG78_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNE22890.1"
                     /translation="MKRKIPLFRILVASAVLFSAAGVFAEPPGPPPEEGGMFGPGNPR
                     EFGKLMDELKLTDAQRAKVKAQHEATKTDAKKYHEQLKPMHEELRALLEANTVDLKAV
                     RAKLQSISNVQLELRMIHIKGRLDFESVLTPDQKAQLKKITKERMDKMREHHGENRPH
                     RGPGAPDDER"
     gene            4785..5321
                     /locus_tag="PLG78_06980"
     CDS             4785..5321
                     /locus_tag="PLG78_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE22891.1"
                     /translation="MTEAELTGAIEKSKVTALKSIRRHLPHDLDGLLDDIVQETYLRY
                     YMTFRDSRPLSDDYLHRWIYVAARNECKRAARKSRREGFAYAKLKPDAEAAANEENTS
                     VNEDMVPQDPENVRLQIEAMPDPFRETFLLRLGGLKMVTIAERLNVTVGTVKSRLARG
                     KRFLSRKGIASEDAGSQS"
     gene            5318..5638
                     /locus_tag="PLG78_06985"
     CDS             5318..5638
                     /locus_tag="PLG78_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22892.1"
                     /translation="MSQIERSPGYRRTEKKILSDSFSAELAARIVRDANARKRAPTQI
                     WTMGLAAAAVVVLAVGLFFYTQRTPDYPGLTSYSAQSVAAADQTNADWNETDSIISSA
                     LENR"
     gene            5724..6635
                     /locus_tag="PLG78_06990"
     CDS             5724..6635
                     /locus_tag="PLG78_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020773820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22893.1"
                     /translation="MTFALLGAFASVEAQSPRCSVFPADHIWNTRVDSLPVHKLSASY
                     VRSIGEKKALKADFGSGLWEGGPIGIPFTTQTGSPVQVQFEYADESDRGPYPLPSKPP
                     IEGGDASNGDRHVIVVDESKCKLYELYNARKTSRGWNAGSGAIFDLRTYVLRPAGWTS
                     ADAAGLPIFPGLVRYDEVASGEIKHAIRFTTNITQKAYVWPARHFASSRKDANLPPMG
                     LRLRLKSSYNMNGFSRETRVILQALKTYGMILADNGSPWFLSGAPDERWNNKTLRELG
                     RVTGENFEAVDATGLMMSADSGQIKKK"
     gene            complement(6674..>7957)
                     /locus_tag="PLG78_06995"
     CDS             complement(6674..>7957)
                     /locus_tag="PLG78_06995"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNE22894.1"
                     /translation="LLRELSPGRPIIHNFMTLFFELDPWKMSEDLDLAGFDHYQMESD
                     PHPISSAWQFRLMASLKKRSFMVLEQQPVQVNWQKVNRRFPMNLLFQWGMQAMFAGAD
                     SMLYFSWQRMYGGAEQYHDGIVSHDIRVDTTEQEELLQFQRSFLSALKTAGIQKIDPL
                     GDVCMVLDFESIWSHDITSQSELFSTRTVVDVAAEFCLSQGLGFSFSPSIESIDPSSR
                     MVFLPAHSFELSEKERSVLRQYMEEGGIVCSFPRSLMKRRDNRMSDLPLHLLHPEDLR
                     VLSYGALLEEEREEFKDEFQGRIWAEKIEPGPELSVLARFTKGLYSGSPAVILQKAGR
                     GKWIHLCTVPERSREFFSWLAIHTLSRIECPPGVQAFPMNTNLGPAIGWINHGTDAEV
                     PWSGKLIQARFPDIMASPAMGRILAAGAIGVLLPD"
BASE COUNT         1935 a         1889 c         2077 g         2056 t
ORIGIN      
        1 tgcttctcca ccgcaacctg ctgttacgat tccaggagga ggacgatctc catccggtct
       61 atctgagaac atgggtggct tgagatgttt ttcgtcgtag atacgtttat agaatcgacc
      121 catggtagga gcggcaatgt gaccgcccgc ctggcctgcg ccgagtgtga cgctcgattt
      181 atcaaaacca aaccagatgg ccgcggcaac atccggattg aatccaacga tccacgcatc
      241 tgaccagcta gaagtagttc ctgttttcgc agccagactt ccagtgaatt gtgccgtttg
      301 tcttacgccc agcgttgctg ttccgtgatc cgcaacagca atcatgatct tgcgtagcaa
      361 gtatgcgagc gcggggtcaa tgatctgaat gcggttttcg tctgtctttg ctttgatgat
      421 tttgcggatg ttctcttcct gattgtagag agtgtttccg ctttgatccg ttacgtatcg
      481 caccgcaaat ggaatgacat ctttgccatc atttcctatg actgcgactg cctttgccat
      541 ttcaaatgga gtgacttcgc ttgaaccgag cgcaagagat ggatctggat gaaatcgatc
      601 tttgtttgtg atctttgtga gtctctgtgc gaattcgatg atcgcgtccg gacctacttt
      661 gtaataaacc tgaatcgcac acgtgttgat ggatagagcg aaagcagcaa atactggcgt
      721 tagaccgcgg aacccttcgt cgtagttacc aggagaccag ttgtttccca cagcatcaat
      781 gctgtagaaa ggagcatcgt tgactgcgga ttgtgtgctg atgagacgct tctcaaatgc
      841 tgcgccataa atgaagattt tgaatgcact cccaggttgc ctgcgcgcct gcaatgcacg
      901 gttgaattga ttctgaggag tgaactcagt tcctcccacc atggaagtga tgtaaccgct
      961 gcgaggttca atcgaaatga atgcgccctg cacctgaagg ttggtcgtat aactgatggt
     1021 tgcttttcta aactcttcga aagcagcagc ttcattgaat gcaggagtgc tgtaaccaag
     1081 cagctgtgta ggctcgatca tttcttcttc gtatgctttg cgaaattgcc cgcgaatatc
     1141 cagacctttc acgtcaggcg atcccacagg caaaatcatg gacagctgac tgaaaatgcc
     1201 gaatagactc atgtccactc cgccttttcc gcccgctttc gcgtacgcgc ggctggtctg
     1261 gtttacttcc gcaacttttt ttagtaattc ttcttctgcg attctttgat gatccagatc
     1321 cagtgtggtg tagattttca acccacccgt gtagagaagc tctggtccca cttcttcgga
     1381 tgcttctaga atctggcgaa catagtccgt aaagaatggc gctttgtcca ggcgatccgc
     1441 ccagatgttt tgcgaaggag attgaaccac aaccctgggc cagtattcat cccagaattt
     1501 tttatgcact gcttccactt gatcttttgg aagatagccg tgatctgcca tctgccgcag
     1561 tacgattttg ttttgtcgca tgcattcgtc aggattcttg aacggagaat attcaacagg
     1621 agacttgggc atacgcgcaa ggagcgctgc ctctgcgaga gtgaggtccg ttactttctt
     1681 attgaagtag agacgcgatg cacaagcgat ccctttgcat ccgtgtccca gatagattac
     1741 attgaagaat acttctagaa tttcgtactt tgtatagttc tcttctattt cgaaggcgag
     1801 cagagtttcg cgtatcttct ggcgaatcgt tctatttctt ttaccttccg cgttcagata
     1861 gatttgcttg gccacctgct gtgtaagagt ggatccaccc tgtctcactt ttccagagat
     1921 tgcgtttttc actgctgcgc gaagaatgcc ggatacatcc agaccaatgt ggttgaagaa
     1981 gttatcatct tctacggata gaaacgcctg gattacatga ggcggaatgt cctggagttg
     2041 gaccagttcc tgtctctggc gaaagagttc tgcaaacacc ttgccattct tgtcgtagag
     2101 tcttgtgggt gtggacggtc tgtaagcgga aaccaggatc aaatccctgg ctctggctac
     2161 ttcgcggacc gccaggccaa gaatcaggcc gccgagaatc gctccggcga agaggctgat
     2221 gaatagcgtc ttctccgtgc cgaggatgat cttcttttgt ttgtctgtca ggtttgcgct
     2281 catttcggga ttggcttgcc ggattctggc ggtcctgctt taaagcgctt atgcaacgct
     2341 gggaaattca ggaaccgcaa gcccctccat taacagaggg agtttttcgg ctgctggttc
     2401 tattttctct cgcttttggt attgaatgga tcgcggcagc cggctttcaa aggccgattg
     2461 actcgcttgc actctctttt ggaccagatt tccagccaac gcagattctc acacatattt
     2521 ttttagtgat tccgcccggg ttctttggcg gtttgagcct ctttttcctt tgtttgatgc
     2581 tctggggctt cggaggggac attgagaggc tctgggggcc gcgccatttt ctaaacctgt
     2641 ttctggcagg gatggccggt tcgatcctgc tgggactgat cgctcatttg agcgcttttc
     2701 cagggctgag cctgcatgga ccgacgggcg gaatatctgc tgtattgctt gcctacgccg
     2761 ttctatggcc ggatcgcaag gcacttttct ttttcgtcat ccccatgcga atgagatggg
     2821 tcattctagt tttcgccgta ctgattgcag tttcttctat ggtggctctg ctgcaggttc
     2881 tgggtggagc tctgggcggc gcgctcttca tctactatta cttgcgaaag gggaatcgta
     2941 cttacggcca atctgtagaa gatagaacca tgactctctc agaaagatgg gaagagttta
     3001 agaaaaaacg acgtctgaac aaaaagaaag cagagattga acgcagaatc gaaatgaaag
     3061 aagaagtgga tcgtcttctt gaaaaaattt cgaagagcgg aatgaagtca ctttccagta
     3121 aagagaaagc tctgctggat cgcgcatcca gggagttatg aaatgagaat cgtatggatt
     3181 ggttcatgga tcgtgtccgt cgcactactg gcaacagttg attgtaaacc caggactttg
     3241 acagaagtcc ctatgccgga ttacaagtcg ttcaggcatg tgaagtcagg cttgcttctc
     3301 aaaggagatg cagacgattt acttacaggg attcaccacg tttatgcaaa cgatgcagcc
     3361 gtagaagcat tgcgaaatcg atctgatttt ccagaagatt cggttctcgt gtttgatttg
     3421 ttcgaacctc tggacactcc agaaggatac aaagagggcg cgcataaatt cacggccgtg
     3481 atgcacaaac attcttcttt taagtccacg ggcggctggg gatacgaagc gttccttccg
     3541 gatggaaaga gagtagtttc agacgcaaat aagatgtgtt tcgaatgcca taaatccgta
     3601 gaatcgcagg gttacgtatt cacgaatgca ggcatgagcg aataacgttc gccaaatccc
     3661 tgacgaaata ttgcaaaggc gtgtttcagt gccgaccgca gatcagcact gaaacgggtt
     3721 tcctatttat tcatctggtc tttgcagttc tgtcccaccg aagaaggatt cttattcatt
     3781 tgttggcgaa tgcacatgta tagttttccg tcttccgttg cggtcgagca gtaagattga
     3841 atctctgtgc tgcagctggc tttgattgcg gggaagcccc ctctgcgggg cttgcattcc
     3901 ggggaaagct gctttgattt ttcggggtct tttagacagg cccccagctt gtccccgtca
     3961 ccgccgtagc ccgggcaaat tcttgccagt tcatccttgc attttgtatt tctttcctgt
     4021 gatagaagcg cgcctgaaac aaacagcgcg atcaacacaa cgatgaattt attcatccaa
     4081 ctgctccttt tttgatatgg gcgatttttt tcagccccgt ggaatacaag caggtcatcc
     4141 ggattttttg cgttcttttt gagaaattat tcgcctggat tgcgtacgca tggatagtct
     4201 ggatcagact gacgtgcgaa atggtgtctc tagaaaatct ggggaacctt ttttcgcctg
     4261 cgggtttata gagacatgaa acgaaaaatc ccccttttca gaattctagt cgcaagtgcc
     4321 gtgctgtttt cagcagcagg cgtctttgct gaacctccgg gccccccgcc agaagaaggc
     4381 ggaatgtttg gaccaggcaa tccacgtgaa ttcggtaaac tcatggatga gctgaagctt
     4441 acggacgcgc agcgtgcgaa agtaaaagcg cagcatgaag ctacaaaaac cgatgcgaag
     4501 aagtatcacg aacagctcaa gcctatgcat gaggaactcc gtgcgcttct cgaagcaaac
     4561 acggtagatt tgaaggcggt tcgagctaaa ttgcagtcca tcagcaatgt tcagctggaa
     4621 cttagaatga ttcacatcaa aggacgtttg gatttcgaat ctgttctgac tccggatcaa
     4681 aaagctcagt tgaagaagat cacgaaggaa agaatggaca agatgcgcga acaccacggg
     4741 gagaatcgtc cccatcgcgg tccgggcgca ccggacgatg aaagatgacg gaagcggaac
     4801 ttacgggcgc tatcgaaaag agcaaggtaa ctgctcttaa gtcgattcgg cgccatctgc
     4861 ctcatgatct tgatggtctc ctggacgaca ttgtccagga gacttacctg cgctattata
     4921 tgacgtttcg cgactcgcgt ccgttaagcg acgactatct gcatcgctgg atctatgtcg
     4981 ctgcgcgaaa cgaatgcaaa cgagcagcgc gaaaatccag acgagaagga tttgcgtatg
     5041 cgaagctgaa gccggatgca gaggcggccg cgaacgaaga aaacacgagt gtgaatgaag
     5101 atatggtgcc gcaggatccg gagaatgttc gcttgcagat agaagcaatg ccggatccgt
     5161 ttcgggaaac atttctgctt cgcctgggtg gattaaagat ggttacgatc gcggagagac
     5221 tgaatgttac tgttgggact gtgaagtcca ggcttgcgcg tggaaagcgc tttctttcca
     5281 gaaagggaat cgcgtctgaa gacgcaggga gtcagtcatg agccagatag aaagaagccc
     5341 gggatacagg cggacggaga agaaaattct ttccgattct ttttcagcag aactcgccgc
     5401 gcgcattgtg cgagatgcaa atgcaaggaa gcgagcgccc actcaaatct ggaccatggg
     5461 actcgctgcc gcagcagtgg ttgtccttgc tgttggtttg tttttttata ctcagagaac
     5521 tccggactat ccaggcctca cttcttattc ggcgcaatct gtagctgctg cagatcaaac
     5581 caacgcagac tggaatgaaa cggattcgat cattagttct gctcttgaga ataggtgata
     5641 caacatgagt gaaaacgtat gagtgaagca attccagatt tccggatgag tgtatgaaga
     5701 tgaatcttag atcatcgtgt tccataacct ttgccctgtt aggtgctttt gcgagtgtgg
     5761 aagcgcagag tccgcgctgc tcggtatttc cggcagatca catctggaat acgcgagtag
     5821 attcgctccc cgtccacaaa ctgtctgcct cctacgttag aagcattggt gaaaagaaag
     5881 cactgaaggc cgattttgga agtggtctat gggaaggcgg gcccatcggg attccgttta
     5941 ctacccagac cggttcacct gttcaggtgc agtttgaata cgcggacgaa agtgaccgcg
     6001 gtccatatcc gcttccatct aaaccgccga tagagggcgg agatgcatct aacggagaca
     6061 gacatgtgat cgttgtagac gaatcaaaat gcaagctata cgaactgtac aatgcgcgaa
     6121 agacgtcgcg cggatggaat gccggatccg gggccatatt cgatttaaga acctacgtac
     6181 ttcggccggc cggatggacg tctgcagatg ctgcgggcct tcccattttt ccaggactgg
     6241 tccgttacga cgaagtggcg tctggcgaaa tcaaacacgc aatccggttt accaccaaca
     6301 ttacgcagaa ggcatacgtc tggccggcgc gacattttgc ttcgagcagg aaagacgcga
     6361 atcttcctcc catgggactc cggctgcggc tcaaatcttc ctacaatatg aatggatttt
     6421 ctcgcgagac gcgcgtgatt ttgcaggcat tgaaaacata cggaatgatc ctcgctgaca
     6481 atggttcccc ctggtttctt tccggagctc ccgatgaaag gtggaacaat aaaactctgc
     6541 gcgagctcgg tcgcgtaacc ggggaaaact ttgaagcggt ggatgcaact ggacttatga
     6601 tgagtgctga ttccggacaa attaagaaga agtaaccgcc acaggttcgg atgcgccgag
     6661 atgtcgcata acgtcagtct ggaagcaata ctccaatcgc acccgcggcg aggatccgcc
     6721 ccattgcagg cgatgccatg atatcgggga agcgagcctg aatgagtttt cctgaccatg
     6781 gcacctctgc atctgttccg tgattgatcc atccaatggc cgggcccaga ttcgtattca
     6841 ttggaaaagc ctgcacgcct ggcgggcatt cgatgcgcga gagtgtatga atggccaacc
     6901 aggaaaagaa ttctctggat cgctcgggca cagtgcacaa atgaatccac tttccgcggc
     6961 cggccttctg cagaatgact gcgggcgaac ctgagtagag cccttttgta aatcgagcga
     7021 gaacggaaag ttcgggacct ggttcgattt tttccgccca gattcgtccc tgaaattcgt
     7081 ctttgaattc ttctctctct tcctcgagga gcgctccata agataggact cgcaagtctt
     7141 ccggatgaag caaatgcagc ggcaaatcgc tcatgcgatt gtcgcgccgt ttcatgaggc
     7201 ttcgaggaaa cgagcaaacg attccgcctt cctccatgta ttgtctcaga acggagcgtt
     7261 ccttttcgct gagttcaaac gaatgcgcgg gtagaaagac catcctggaa gaaggatcga
     7321 tggattcaat ggaaggagaa aatgaaaaac caagaccctg cgaaaggcag aattccgcgg
     7381 ccacatcgac cacggtcctt gtagaaaaca gttcagactg cgaggtgatg tcatggctcc
     7441 agatggattc aaaatcaaga accatgcaaa cgtcgcctaa cggatcgatc ttctgaatgc
     7501 cggccgtttt gagggcggaa agaaagcttc tttggaattg aagaagctct tcttgctctg
     7561 ttgtgtctac gcgaatgtcg tgcgatacaa ttccgtcgtg gtactgctcg gcaccaccgt
     7621 acattctttg ccaggaaaaa tagagcatgg agtccgcgcc tgcgaacatg gcctgcattc
     7681 cccactggaa gagcaggttc ataggaaagc gacgattgac tttctgccag tttacctgta
     7741 cgggttgttg ctcaagaacc atgaaggatc tcttcttaag agaagccatc agacgaaatt
     7801 gccatgcgga gctgatcggg tgcggatctg attccatttg atagtgatca aatccagcaa
     7861 gatccagatc ttccgacatt ttccacggat ccagttcaaa gaagagagtc atgaagttgt
     7921 gaatgatcgg acgacctggc gaaagctcgc gaagaag
//
LOCUS       DAOQJL010000118         7926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4962_length_7926_cov_4.742472, whole genome shotgun sequence.
ACCESSION   DAOQJL010000118 DAOQJL010000000
VERSION     DAOQJL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4962_length_7926_cov_4.742472"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLG78_07000"
     CDS             <1..723
                     /locus_tag="PLG78_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNE22895.1"
                     /translation="IPEIFIVDEALGVGDASFQMKSMRRMRELQEQGTTIAFVSHDAS
                     TVRSLCTRAILLDKGRIVAEGSPAQVYDIYSGMIGFADEAVEANASFAERSGSGELRF
                     AEAWIENSSGKRADTFRSGEGARIVLEIEASETIENPTIGIHFRDASSHELFGINTAL
                     LGIDTGIFVSGQKRRVAFTMPLDFGPGLCTLGTSIHQFEIHTGSCYDWVNHAITLRIE
                     PDPSYRFSGSARLRASFLSSVS"
     gene            complement(716..1108)
                     /locus_tag="PLG78_07005"
     CDS             complement(716..1108)
                     /locus_tag="PLG78_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22896.1"
                     /translation="MKRIFLLLLTFSAWGLQIQAEQTTAPSSSGKKPFVRPVLVPSFG
                     EPASGRLGWEQAVSYCLEQKSRLPTVEELKLAFQNGLLKTWEQQGDYWTLSDDADQSI
                     AYSFITKFGLAHAESKGHGNLVRCIKPN"
     gene            complement(1140..2342)
                     /locus_tag="PLG78_07010"
     CDS             complement(1140..2342)
                     /locus_tag="PLG78_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE22897.1"
                     /translation="MSIKQKLGLYKPSLQPIPEVRIQRSFGTVKCNELGPYVLERTIS
                     TDSLKRGDRLLSDYHPFRVSGILELALVRENHPVHPEDLLFLDTETTGLSRGAGNFPF
                     LVGLGYFENGSLVIRQILMTSPAGEKQFLDAIQEEVRKHSHLVTYNGKSFDIPLIKNR
                     LILQRDRLHPPVLHFDLFHILKRLFPHRRLGRYRQKDLEAELLGFSREDDIEGAQVPQ
                     IYFDFVKYGTDTGMEKVIEHNRLDLEGMMLLFLEAVRVYTDLDSSRASLRSGLARILA
                     HNHRYFEALDAAMEIQDLLLDGKEGFHYRDALFRAWLERRAGNYEEAASTYETIAERC
                     MCDYSRLNLVRILEYRLNRPHQAIEHVDVLISRAPPPDLPSLARRRARLDMKAKKLDE
                     RRGRESSA"
     gene            complement(2342..3148)
                     /locus_tag="PLG78_07015"
     CDS             complement(2342..3148)
                     /locus_tag="PLG78_07015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE22898.1"
                     /translation="MPRAGNSLLSVCIIALNEELRLPRCLESLSFADEIVVLDGGSVD
                     GTIKLAKSYPRVRVEKRRFDDFVRQKNHVISLAKYPWILVVDADEVVTPALRDEISQV
                     IGNNISAEGYRVPRMTFYLGKWIKHCGWFPDYNIRLFRRGRARFEGGTVHEKVAVDGT
                     IGTLKGHLQHYSYRTTSDHLIRMDQYSTLLAEDKFLKGKRSGPLLAGYKSLSKFLLIY
                     FWKLGFLDGRPGFVIAVMGAYYNFLKYIKLWELRRGLRKPGDRVRYPGNV"
     gene            complement(3148..3855)
                     /locus_tag="PLG78_07020"
     CDS             complement(3148..3855)
                     /locus_tag="PLG78_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE22899.1"
                     /translation="MGPSLQEINDNIAIQVSNGQYLTLKTHRMTEMVEDHIKHAIESI
                     LAKTNKSNLIHVIYTILKELVINGCKANQKRVFFEERGYNISDSQQYAVGNEEYKKIF
                     SEEMALEYGIKAKAKGLYCLIDFLFDENGLTIEVSNNTTITPQEEASMREKLKKAMGY
                     NDIAEFYLDQAMSGGETEGAGLGLALIIILLKGENVDPKYFRIMIDKERTVARMEIPF
                     TDRFVSKRDLGSKVVQG"
     gene            3940..4419
                     /gene="sixA"
                     /locus_tag="PLG78_07025"
     CDS             3940..4419
                     /gene="sixA"
                     /locus_tag="PLG78_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNE22900.1"
                     /translation="MKLLLARHAEAESSTPDSERSLTEKGKSDARRMARVLRQTGWQF
                     EEILCSPLRRTVQTAELYGAGLGTSAKNAVQAVPFLAPTADRTRAIEHLGEMGIRGAA
                     LWVFHMPDVAEVASWLLGIPAASLYVTPGTIIALNLAFPVPGKAMLVWSMQPEHLPE"
     gene            4419..5945
                     /locus_tag="PLG78_07030"
     CDS             4419..5945
                     /locus_tag="PLG78_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22901.1"
                     /translation="MRFWISCALFVWACACLEGTLSHNLYSSRSPELSGLHHYFNADA
                     MYLPALFQDWQAGKDLHGWTLPPAPYLFPDLPIYFTFLKFFPFKSAAFLAGIFLLLFN
                     LFSVLYFAKRGLRVKRSNSSVLLTGAILLCVLARCAIAEEFYSLIVPTFHAGVLGIAF
                     LTFGWMLQSEHSRSRLPHLVATMTLASLSDALIIALVLPAAVSWILVRRTRSSVLAAL
                     AIATGLILGRLAVGPMSDYLAPIFRVSPERDVLHFLTSEHALSTIVNDLSQPVYLLPV
                     AFAVVMIAYAMSVKQRQGLILPAVAILSAGVAASAFSHQVGLPRMEPRYALMPLFAAG
                     TLAGMWLFRSRLFFVLAAILVSVTIFKTRTEAGIHPIEEAANCLSGLSETHGIKKGFA
                     DYWHSKQYSVLTDRRVHIEQIHVNAGARIVEPRMWINNLDGYFPENRPEDFDFLIAND
                     LPRDVLLERFGEPVLKRSCGHLDVWIYRKDNRGVVPFTFDSFQLWRNAIGRPARIKDK
                     "
     gene            complement(5948..7096)
                     /locus_tag="PLG78_07035"
     CDS             complement(5948..7096)
                     /locus_tag="PLG78_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE22902.1"
                     /translation="MNKKVALVIQRYAPDTAGSEQHARITASLLAEHYEVEVLTTTAS
                     DMVTWRNHFSPGETAEGNIKIKRFEVTSGRQKTWEGLYALMLRRLKTHGETGWPLSLE
                     EEWIRLQGPHSEGLLAYLSEHQQNYDAVIFYTYLYSPTYFGTACVEKNRSLLMPTLHD
                     EPAARLACYAHAARRVKRIVWNTFAEQKLGEKLWGNLPGTMTGLPIDIPDPVSPVRAA
                     PYVLYSGRIEPGKGCDDLVGMIQNSSARVDLRLTGTKYMKLSGSRIHYEGYVTAQKKL
                     ELLAGSICFAMPSPNESLSIATLEAMAAGVPVLVNGRNPVLREHVEESGGGLCYESSG
                     EFDEAILTLKDNADLRSTMGQKGRAYVQRRFSHRSIRDAWISAIESGA"
     gene            complement(7093..>7926)
                     /locus_tag="PLG78_07040"
     CDS             complement(7093..>7926)
                     /locus_tag="PLG78_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE22903.1"
                     /translation="ASPARRIIKYHNVTPASFFEAHNNDYANICRKTREALRPLVSSA
                     HLVLCDSTFNLEDIVLEGATRNQCKVVPPFHRLESRDWLDLDPLWVERFTRPGHPMFL
                     SVGRIVPNKALEKCIEALGVMQSRYGIPAKLVLAGRIDRRLGSYIRFLEKKSVEHCIP
                     GTVIFADHVSDTRLRTLFLCAVAYLVTSEHEGFCVPVIEAMKSRLPVIACGKTAVAET
                     LGGQGIYTETFDAHLFASAMERLVKSKESCAIIADHASHAYETRFHSEELFRIFLEAL
                     S"
BASE COUNT         1905 a         2041 c         1934 g         2046 t
ORIGIN      
        1 attcctgaaa tcttcattgt agacgaagca cttggtgtag gcgacgcctc gtttcaaatg
       61 aaatctatga ggagaatgcg agagctccag gagcagggaa ccaccatagc ttttgtaagt
      121 catgacgcat cgactgtccg aagcctctgc acgagagcta tcctgctcga taaaggtaga
      181 atcgtggcag agggaagccc cgcgcaggtg tacgacattt acagcggcat gattggattt
      241 gcagacgaag cagtagaggc caatgcgtca ttcgcagaga gaagtggatc cggagaattg
      301 cgattcgcgg aagcatggat agaaaactca tccggaaaga gagcggacac atttcgctca
      361 ggggaaggcg cgcgaatcgt tcttgaaatt gaagcgtccg aaactataga aaatccaact
      421 attggaattc acttccgcga cgcatcaagt catgaacttt ttggaatcaa cactgcattg
      481 cttgggatag atactgggat ttttgtatcg ggacaaaaaa ggcgcgtcgc attcaccatg
      541 cctctggatt ttggacccgg cctgtgcact cttggcacat ccattcatca atttgaaatc
      601 cacaccggct cctgttacga ctgggtgaat catgcaatta cacttcgaat cgaaccggat
      661 ccgtcttatc gtttttcagg aagcgcaaga ttgcgcgcaa gcttcctgag cagtgtcagt
      721 taggtttgat gcaacgaacc agatttccgt gccctttgga ttctgcgtgc gccagtccaa
      781 actttgtgat gaaagaatag gcgatcgatt gatctgcatc gtcggataac gtccagtagt
      841 ctccctgttg ctcccaggtt ttcaataacc cgttctgaaa cgccagtttc aattcttcca
      901 cagtaggaag acgagacttt tgctccaggc aataggacac tgcctgctcc catcccaatc
      961 ttccgctggc aggttcaccg aaagatggaa ccagcaccgg tcgaacaaat ggtttcttac
     1021 cggatgaaga aggtgcagtg gtttgctctg cctgaatttg caggccccat gctgagaaag
     1081 tcaacaaaag caggaagatg cgtttcatga ccttagactt tcctccccct tgctcctggt
     1141 caagcggaag actcacggcc gcgacgttca tccagtttct ttgctttcat atccaggcgg
     1201 gcgcgacggc gggcaaggga tggcagatct ggtggcggcg ctctggaaat caaaacgtct
     1261 acatgctcga tcgcctgatg agggcgattc aatctgtatt ccagaatcct tacaaggttc
     1321 aatctggaat agtcgcacat gcagcgctct gctatggttt cgtaggtgga cgctgcctct
     1381 tcgtaattgc ctgcacggcg ctccagccat gcgcgaaaca gtgcatcgcg ataatgaaat
     1441 ccttcttttc cgtccagcaa cagatcctga atttccattg ccgcatccag agcttcaaaa
     1501 tagcgatgat tgtgggcgag aattcgcgca aggccagaac gaagagatgc acgcgaagaa
     1561 tcaagatccg tatacactcg cactgcttct agaaaaagaa gcatcattcc ttccagatcg
     1621 agtcgattgt gttcgatgac tttttccatt ccagtgtccg taccatattt cacgaaatcg
     1681 aaatatatct gaggcacttg agcgccctca atgtcatctt cgcgtgaaaa acccagaagc
     1741 tcagcctcca gatccttctg tctgtatcgt cccagtctgc gatgaggaaa gagtctcttt
     1801 agaatatgaa acaaatcgaa atgtaaaaca ggcggatgca gtcgatctct ctgcaggatc
     1861 agacgattct tgatcagagg aatatcaaaa gactttccgt tgtaagttac aagatgagag
     1921 tgcttgcgaa cttcttcctg gattgcgtcc aggaattgct tttctcctgc gggagaggtc
     1981 atgagaatct ggcgaatcac aagggatccg ttttcgaaat atccaaggcc aaccagaaac
     2041 ggaaaatttc ccgcccctcg cgacagaccc gttgtctctg tatccagaaa aagcaagtct
     2101 tccggatgta cgggatgatt ttcgcgcacg agagcaagtt ccagaatgcc acttacgcga
     2161 aagggatgat agtcagaaag aaggcgatct ccgcgcttta gagaatccgt ggaaatagtt
     2221 ctttcgagga cgtacggtcc gagttcgtta cacttaaccg tcccaaaact tcgctgaatg
     2281 cgaacttctg gaatcggttg cagagatggt ttgtacagac caagtttttg cttgatggac
     2341 attagacatt tccggggtac cgaactctat ctccgggttt acgcaatccc cggcgcaact
     2401 cccagagttt gatatacttt agaaagttat agtatgctcc catcacggcg atcacaaaac
     2461 cagggcgacc gtccaaaaac cccagcttcc agaaatagat gagaaggaac ttactcaaac
     2521 tcttgtagcc cgcaaggagt ggccccgatc gctttccttt cagaaattta tcttccgcaa
     2581 gcagagtgga gtattgatcc attcgaatga gatgatcact tgtggttctg taagaataat
     2641 gctgcagatg gcctttcagg gtaccaatcg ttccatccac tgcgactttt tcgtgaaccg
     2701 tgccgccttc aaatcgcgct cgcccgcggc gaaagagtct gatgttgtaa tcaggaaacc
     2761 atccgcaatg tttgatccat tttcccagat agaaagtcat tctgggcacg cgataccctt
     2821 ctgcggatat attatttcca atcacctgcg aaatttcgtc acggagtgca ggagtgacta
     2881 cttcatcggc gtccaccacc agaatccacg gatacttggc gagcgaaatg acatgattct
     2941 tctgacgcac aaagtcgtcg aatctgcgct tttccactcg gactcgtggg taggatttgg
     3001 cgagcttgat ggttccatcc acagatccgc cgtccaggac gacaatttca tctgcaaacg
     3061 aaagagattc caggcagcgt ggaagacgca gttcctcgtt gagggctatg atgcagacgg
     3121 agaggagtga attgcccgcg cggggcatca accctgaact accttggagc ccaggtctct
     3181 cttgcttaca aagcgatccg tgaatggaat ttccatccgt gccacggttc gctctttgtc
     3241 gatcatgatg cgaaagtatt ttggatctac gttctcaccc ttcaaaagaa tgatgatcaa
     3301 cgccagacca agtccagcac cttccgtttc tccgcccgac attgcctgat cgagataaaa
     3361 ttcagcgatg tcattgtaac ccattgcctt tttcagcttc tcgcgcatgc tcgcttcttc
     3421 ttgcggtgtg atggttgtgt tgttgcttac ttcaatggtc agaccatttt catcaaaaag
     3481 aaaatcgatg aggcaataga gccctttggc cttagctttg attccatact cgagcgccat
     3541 ctcttcgctg aagatcttct tgtactcttc atttccaact gcgtattgct gcgaatccga
     3601 gatgttgtaa ccacgctctt caaaaaaaac tctcttttga tttgctttgc atccgttgat
     3661 cacgagctct ttgaggatcg tataaatcac gtggatcaga ttgcttttgt ttgtcttcgc
     3721 gaggatgctt tcaatcgcat gcttgatatg gtcttctacc atttcagtca ttcgatgtgt
     3781 cttgagggtc agatactgcc cgttggacac ctgaattgct atattatcat taatttcctg
     3841 tagagacgga cccacgcaga cacactcgac ccaaaaacct ggtttgtcaa ggcatgcttt
     3901 ctgctttacg gccccggtac ggacaatttc gcgtacatta tgaagctact cctggcccgg
     3961 cacgcggagg cagagtcgtc cacgccagat tcagaaagaa gcctgactga aaagggaaaa
     4021 agcgacgcgc gccgaatggc tcgagtactg cggcagaccg gttggcagtt cgaggaaatt
     4081 ctatgcagcc cgctacgcag aaccgttcaa actgcagagc tttatggagc gggtcttgga
     4141 acgagtgcga agaatgccgt gcaggccgta ccttttctcg cgccaacagc ggaccgcaca
     4201 cgtgcgattg aacatctagg cgagatgggg attcgaggag cagctctctg ggtatttcat
     4261 atgccggatg ttgcggaagt ggcgtcatgg cttttaggaa ttccggcagc atcgctctat
     4321 gttacgccgg gtacaatcat cgcgctcaac cttgcattcc ccgttccagg caaagcaatg
     4381 ctcgtctggt ccatgcaacc ggagcatctt ccagagtgat gcgcttctgg atctcatgcg
     4441 cattgtttgt ctgggcctgt gcttgcctgg agggaactct ttcgcacaat ttatactcta
     4501 gccgttcgcc cgaattgtcc ggacttcatc actatttcaa tgcagatgca atgtatctac
     4561 ccgcactctt tcaggactgg caggcaggta aagatctaca cggatggact ctgccgcccg
     4621 caccgtattt atttccggac cttccgattt actttacatt tcttaagttt ttcccgttta
     4681 agtccgccgc gttcctggca ggcatctttc ttctattgtt taatttattt agtgttctgt
     4741 actttgcaaa aaggggcctt cgagttaagc gatccaattc atctgtgcta ctcacgggtg
     4801 ccattctatt gtgtgtcctc gcgcgctgcg ccatcgcgga ggaattctat tcgttgattg
     4861 ttcccacatt tcacgcaggg gtcctgggaa ttgcatttct aacattcggt tggatgcttc
     4921 aatctgaaca ttccagatcc agactaccac atcttgtggc gacaatgact ctcgcttctc
     4981 tttcagatgc tctcatcatt gccctggttc ttcctgcggc ggtttcctgg attttagtgc
     5041 gaagaacccg atcttctgtt ctcgcggccc tggccattgc cacaggattg attctgggtc
     5101 gcctcgcagt gggccccatg tcagactacc tcgcaccgat ctttcgcgtc tcaccggaaa
     5161 gggatgttct tcacttcctt acttcggagc acgcgctatc cacgatcgtg aatgatctct
     5221 cgcagccagt gtatttactt cccgttgcat ttgcagtcgt catgatcgcg tacgccatgt
     5281 cggtaaaaca aaggcagggg ctcatcctcc ccgctgtcgc catcctttca gcaggtgtgg
     5341 ccgcatccgc tttcagtcac caggttggac ttccgcgcat ggagccacgt tatgcgctca
     5401 tgcctttgtt cgcggccgga actctggctg gtatgtggct ctttcgatcg agactatttt
     5461 ttgttctggc agcgatcctt gtctctgtta ccattttcaa gactcgcaca gaagcaggga
     5521 tccatccgat tgaagaggcg gcgaattgtt tgagtggatt atccgaaact catggcatca
     5581 agaaaggatt cgcagactac tggcattcca aacagtattc ggttctgaca gacagacgcg
     5641 tccatatcga acagatccat gtaaatgcag gagcgcgcat tgtagaacct cgcatgtgga
     5701 tcaacaacct ggacggatat ttccctgaga acaggccgga ggattttgat tttctgatcg
     5761 cgaatgatct accgcgagat gtattgctcg aacgatttgg tgaaccagtt ctcaaacgtt
     5821 cctgtggcca cctggatgtc tggatctata gaaaagacaa ccgcggagta gttcccttca
     5881 cctttgattc ttttcaactc tggagaaatg caatcggaag acccgccaga atcaaggaca
     5941 aataggatca ggctccgctt tcgatcgcgg aaatccacgc gtcgcgaatt gagcgatggc
     6001 tgaatcgcct ttgcacatag gcgcggccct tctgtcccat ggtggaccga aggtcagcgt
     6061 tgtctttgag cgtaagaatc gcttcgtcaa attctcctga cgattcgtag caaagtccgc
     6121 cgccgctttc ctccacatgt tcgcgcagaa ccggatttct accatttaca agaacaggca
     6181 caccggccgc cattgcttcg agagtggcaa tgctcagact ttcattgggg gatggcatag
     6241 caaaacaaat gcttccggcc aggagttcta gtttcttctg agcagtgacg tacccttcat
     6301 agtggatgcg agagccggag agtttcatgt acttagtccc ggtgagtcga aggtccacgc
     6361 gcgcagaaga attctgaatc attccaacga gatcgtcaca ccccttcccg ggctcaatgc
     6421 gacctgagta caagacatag ggcgcagcac gaaccgggct aacgggatcc ggaatgtcga
     6481 ttggaagacc tgtcatggtt cccggtaaat ttccccagag tttctcgcct aacttttgtt
     6541 ctgcgaaggt gttccagaca atgcgcttca cgcgacgagc ggcatgcgca tagcaggcaa
     6601 gccttgccgc tggctcatca tgtagagtgg gcataagaag agatctgttc ttttctacgc
     6661 aggcagtccc gaagtatgtg ggtgaatata gatatgtgta gaagataacg gcatcgtagt
     6721 tttgttgatg ttcgctcaag tatgcgagga gtccttcgct gtgcggtccc tgaagacgaa
     6781 tccattcttc ttctagagag agaggccatc cagtttctcc gtgcgtcttc aaacggcgca
     6841 gcatcaacgc gtagagtcct tcccaggtct tttgccgacc actcgtgacc tcaaagcgtt
     6901 tgatcttgat attaccctcg gctgtctccc ccggagagaa atgattcctc caggtcacca
     6961 tgtctgaggc agtcgtggtc aacacttcta cctcgtagtg ctccgcaaga agactagctg
     7021 taatgcgagc gtgctgttcg gatccagctg tatccggagc gtacctctgg atcacaaggg
     7081 ccaccttctt attcatgaaa gtgcctctaa aaaaatgcgg aaaagctctt cagagtgaaa
     7141 gcgagtctca tacgcatgcg atgcatgatc ggcgatgatg gcgcaactct ctttgctttt
     7201 caccaatctt tccatggcgg atgcaaacag gtgagcatca aacgtctcag tatagattcc
     7261 ctgacctccc agagtctcgg caacggcggt ctttccgcac gcaatcacag gcagacgcga
     7321 cttcatcgct tcaatcactg gcacgcaaaa tccttcgtgc tcgcttgtga ccagatacgc
     7381 aaccgcgcat aaaaacaagg tgcgaagacg agtgtctgaa acgtggtctg caaagatcac
     7441 tgttccgggg atgcaatgtt ctacagactt tttttcaaga aagcgtatgt aagagcccag
     7501 cctgcgatcg attcttccgg ccagaaccag cttcgcagga attccgtatc tactctgcat
     7561 gacacccagc gcttcgatgc atttctcaag agccttgttg ggaacaatgc gcccaacact
     7621 caaaaacatg ggatggccag gccgggtgaa tcgttccacc cagagcggat ccaggtccag
     7681 ccaatccctg gattcgagtc tgtgaaacgg cgggaccacc ttacattggt tacgcgtagc
     7741 tccctcgagc acgatatctt ccaggttgaa agtagaatca caaagcacca ggtgcgccga
     7801 cgagactaga ggacgaagag cttctcgtgt cttcctgcat atgttcgcat aatcgttgtt
     7861 atgtgcttcg aaaaaggatg ccggggttac attgtgatat ttgataatgc gcctcgcagg
     7921 acttgc
//
LOCUS       DAOQJL010000119         7902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4981_length_7902_cov_4.739391, whole genome shotgun sequence.
ACCESSION   DAOQJL010000119 DAOQJL010000000
VERSION     DAOQJL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_7902_cov_4.739391"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /gene="rplE"
                     /locus_tag="PLG78_07045"
     CDS             complement(<1..225)
                     /gene="rplE"
                     /locus_tag="PLG78_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE22904.1"
                     /translation="MSLLKKKYAESVIPQLQKQFAYSSVMQVPRFEKVVLNYGSGEAA
                     ANPKQFESAIEEMAQVSGQKPVKTIARKSIA"
     gene            complement(225..551)
                     /gene="rplX"
                     /locus_tag="PLG78_07050"
     CDS             complement(225..551)
                     /gene="rplX"
                     /locus_tag="PLG78_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNE22905.1"
                     /translation="MAAEFSTKLRKDDEVIVIAGKQKGKRGKIMSLDKAKARIIVQGV
                     NRVKRFQRPSQENPKGGTVELEQSMPISNVLFYDSKSKKGVRLGYKIQPDGKKVRVTR
                     PDGREV"
     gene            complement(552..944)
                     /gene="rplN"
                     /locus_tag="PLG78_07055"
     CDS             complement(552..944)
                     /gene="rplN"
                     /locus_tag="PLG78_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNE22906.1"
                     /translation="MIQQQTYLDVADNTGARKVMCIKVLGGSRKRYAGVGDIIVVAVK
                     DAIPAYGIKDATGKKVHNKVVQKAVVVRTRKEIRRPDGSIIRFDDNACAIIDDKMNPK
                     GTRIFGPVARELRDRKFLKIVSLAPEVL"
     gene            complement(941..1210)
                     /gene="rpsQ"
                     /locus_tag="PLG78_07060"
     CDS             complement(941..1210)
                     /gene="rpsQ"
                     /locus_tag="PLG78_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNE22907.1"
                     /translation="MENTTKRTAKVRLIQGRVVSDTMDKTRVILLESRKMHPLIKKPL
                     VKSHRIKIHDAKNESKLGDVVLAVETRPLSRDKRHRLFKILEKAE"
     gene            complement(1220..1507)
                     /gene="rpmC"
                     /locus_tag="PLG78_07065"
     CDS             complement(1220..1507)
                     /gene="rpmC"
                     /locus_tag="PLG78_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNE22908.1"
                     /translation="MARALHELADAELHKLVDDSQVEIRELRFQFGVARSLQNPSQIR
                     NLRRTIAGALTVLNLRKLGKAEQKAPTGKPAKEKKKAETKPQAKEKKKAKA"
     gene            complement(1507..1920)
                     /gene="rplP"
                     /locus_tag="PLG78_07070"
     CDS             complement(1507..1920)
                     /gene="rplP"
                     /locus_tag="PLG78_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNE22909.1"
                     /translation="MLAPQRVKYRKRQRGRLRGLSQRGSNVSFGEYGLKALDSGRLTA
                     RQIEAARRAMTRKVKRGGKLWIRIFPDTPVSRKPAETRMGSGKGNPEFWVSVIYAGRV
                     LFEISGVEEALAREALDLAAHKLPIRTVFVKKELI"
     gene            complement(1932..2609)
                     /gene="rpsC"
                     /locus_tag="PLG78_07075"
     CDS             complement(1932..2609)
                     /gene="rpsC"
                     /locus_tag="PLG78_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNE22910.1"
                     /translation="MGQKVNPVGLRVGINRTWDSIWFAKESYRQNLHEDLMIRKMVQK
                     KFKRAGVVKIVIERFPEKIGINIHTVRPGTIIGPKGASIEALKAEVRKKITKPLNISI
                     VEVKKPETIAQTVGESIAQQLEQRMPFRRVMKQALRGAMRGGVEGVKIMVSGRLNGAD
                     MARQEQYKDGRVPLHTLRARVDYAHAEARTTYGIIGVKVWTYHGDALPGLEKAEDDDY
                     AVRRKTG"
     gene            complement(2617..2952)
                     /gene="rplV"
                     /locus_tag="PLG78_07080"
     CDS             complement(2617..2952)
                     /gene="rplV"
                     /locus_tag="PLG78_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNE22911.1"
                     /translation="METKSTGKFLLSAPRKIRLVADEIRGYSYPEAMDILRFIPRKGS
                     RYIESVLKTARSNAVSLQKDLREEDLYIKKVYVDMGPTLKRFRPRARGRGMRRLKRMS
                     HITIVLSNE"
     gene            complement(2955..3245)
                     /gene="rpsS"
                     /locus_tag="PLG78_07085"
     CDS             complement(2955..3245)
                     /gene="rpsS"
                     /locus_tag="PLG78_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNE22912.1"
                     /translation="MSRSLKKGPYIAEHLMAKITKLNSENQKKPFKSWSRRSTVFPEM
                     IGHTILLYNGKSFIPVYIHENMVGHRLGEFAPTRTFRGHTSLDKKIKAKPGA"
     gene            complement(3245..4087)
                     /gene="rplB"
                     /locus_tag="PLG78_07090"
     CDS             complement(3245..4087)
                     /gene="rplB"
                     /locus_tag="PLG78_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNE22913.1"
                     /translation="MPVKRFKPVTKTLRYQTVLDFSELSKVEPRKSLLETVHYRAGRA
                     NSGSIAVRRKGGRHKRMYRIIDFKRNKLDIQGVVKSIEYDPNRSANIALIQYPDGEAR
                     YILSPDGLKVGQPVVAGKGAPIRPGNSLPLGEIPPGTNIHNIELRAGRGGQIARSAGA
                     FATVSGRDQEYIIIKLPSGEVRKIHEACNATVGIVGNKDHNLVSLGKAGRARWLGRRP
                     KVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGQPTRGYKTRKKRKTSDRFIVQRRKNK
                     RIGE"
     gene            complement(4101..4397)
                     /gene="rplW"
                     /locus_tag="PLG78_07095"
     CDS             complement(4101..4397)
                     /gene="rplW"
                     /locus_tag="PLG78_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNE22914.1"
                     /translation="MNLNTVIQRPHITEKSEAIKNIGKEGERYVLRIHPDANKELVRQ
                     ALHQLFKVKAVDVKIINVRGKLKRFRQGKTRMPSWKKAIVVLEGGQKIDFARTA"
     gene            complement(4394..5041)
                     /gene="rplD"
                     /locus_tag="PLG78_07100"
     CDS             complement(4394..5041)
                     /gene="rplD"
                     /locus_tag="PLG78_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNE22915.1"
                     /translation="MKVKKFTAQGAQSGTGELADAIFAAPVSEAAIHSVIRTENRNRR
                     HGTHKTKDFAEVSGGGKKPWRQKGTGHARQGSIRAPQWRKGGIVFGPNPRDYRIGLND
                     RLRRLGIVSILAKKAQREEVGIIDALQAPEFSTKAIVSIFKKAGFADSTVVYVTDVSD
                     LKLQKSFGNLPNAKIMHAQRLNAPELYYSDFLFFSEGALKYLEAHYTLKRKRKPA"
     gene            complement(5038..5652)
                     /gene="rplC"
                     /locus_tag="PLG78_07105"
     CDS             complement(5038..5652)
                     /gene="rplC"
                     /locus_tag="PLG78_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNE22916.1"
                     /translation="MATGLLAKKIGMTQIYDETGKLFPVSVLRAGPCVVSQVKTVEKD
                     GYAAVQIGFDALREKLLSKGEIGHFKKAGIAPQRILAEFRDMEGQSGQEINVSVFNAG
                     DTVRVTGISKGKGFQGSMKRHGFGGGPAAHGSKFHRAPGSLGANTFPAEVWRGKRMPG
                     RTGGDQITLRNVKIVAVDAENNLLFVKGAVPGARTALLRIEVTK"
     gene            complement(5655..5960)
                     /gene="rpsJ"
                     /locus_tag="PLG78_07110"
     CDS             complement(5655..5960)
                     /gene="rpsJ"
                     /locus_tag="PLG78_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNE22917.1"
                     /translation="MAQRIRVKLKAFDHKLIDRSAAEIVATARRTGAEISGPIPLPTR
                     TEKFTVLRSVHVNKKSREQFEIRTYKRLIDIMNTNPDTVDALMKLQLPAGVSVDIKS"
     gene            complement(5978..7180)
                     /gene="tuf"
                     /locus_tag="PLG78_07115"
     CDS             complement(5978..7180)
                     /gene="tuf"
                     /locus_tag="PLG78_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNE22918.1"
                     /translation="MAKEKFDRSKTHVNVGTIGHVDHGKTTLTAAITTVLAKKFGGKN
                     KAVAYDQIDNAPEEKARGITIATSHQEYESEKRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSATDGAMPQTREHILLARQVGVPYIVVFLNKVDMIAEGEREEMVELVQEEVK
                     ELLVKYGYPAETPFVRGSGLKALEGEDSELGTQAIIKLVETLDTYVPDPKRVLDKDFL
                     MPVEDVFTITGRGTVVTGRVEQGKISVNEEVEIVGIKDTVKTVVTGIEMFRKLLDHGQ
                     AGDNVGLLLRGIKKEDVERGQVVAKPGSITPHKKFKAEIYVLTKEEGGRHTPFFNNYR
                     PQFYFRTTDVTGTIVLPKGREMVMPGDNVSIDCELITPIAMDKGLKFAIREGGRTVGS
                     GVVAEISE"
     gene            complement(7276..>7902)
                     /gene="fusA"
                     /locus_tag="PLG78_07120"
     CDS             complement(7276..>7902)
                     /gene="fusA"
                     /locus_tag="PLG78_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775062.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNE22919.1"
                     /translation="TIRQQAEEEGKYIRQTGGRGQYGHCWLRVMPNEPGKGYEFVNKV
                     VGGTVPREYIPAIDKGCNEALQSGVLAGYPMVDVKVEVFDGSYHDVDSSEMAFKIAAS
                     MGFKEACRKAKPVLLEPVMSVEVVTPEDYMGEVVGDLNRRRGRINAMEQRGNARVVQA
                     DVPLAEMFGYATDLRSATQGRAAYTMQFKHYEEVPNSVAQEIMAKAAV"
BASE COUNT         1667 a         2114 c         1890 g         2231 t
ORIGIN      
        1 tgcaatggac ttgcgagcga ttgttttcac tggcttctgg ccgcttacct gcgccatttc
       61 ttcaatcgca ctttcaaatt gctttggatt ggcagccgct tcgccggatc cgtagttcag
      121 aacaaccttt tcaaatcgcg gaacttgcat cacggacgaa tacgcgaact gcttctggag
      181 ctgcggaatc acgctctctg cgtatttctt tttaagaagg gacattacac ttctctcccg
      241 tcagggcgag taacgcggac tttttttccg tccggctgga tcttgtaacc cagacgcacg
      301 cccttcttcg acttagagtc ataaaatagt acattggaaa tcggcatgct ttgctcgagc
      361 tcaactgtgc cgcctttcgg attttcctgg cttgggcgct ggaaacgctt cacacgattc
      421 acaccctgca cgatgatgcg cgccttagct ttgtccagag acatgatctt tccgcgcttt
      481 cctttttgct tgcccgcaat gacgatcact tcgtcgtctt tgcgaagctt ggttgagaat
      541 tcagcggcca tttagagcac ctccggagcg agggatacga tcttaagaaa tttgcgatca
      601 cgaagttcgc gggctaccgg tccaaagata cgagtacctt tgggattcat cttatcgtcg
      661 atgatcgcgc atgcgttgtc atcgaaacgg atgatcgagc catctggacg acggatctct
      721 ttgcgagtgc gaaccacgac tgccttctga acaaccttgt tatgaacttt cttgccagtt
      781 gcgtctttga tcccgtatgc aggaattgca tctttcacgg cgacaacgat gatatcacct
      841 acaccggcgt aacgcttgcg cgatccaccg agtactttga tacacattac tttgcgggcg
      901 ccagtgttat ccgcaacgtc tagatatgtc tgctgctgaa tcattctgct ttctccagga
      961 tcttaaagag gcgatgacgc ttgtcgcggg acagtggacg agtttcgact gcgagcacca
     1021 catccccgag tttggattca tttttcgcat cgtgaatctt gattctgtgc gatttaacaa
     1081 gaggcttctt gatcagcggg tgcatcttgc gcgattccag aagaataacg cgcgtcttgt
     1141 ccatcgtgtc agaaacgact cgaccttgaa tcagacgcac cttcgcggtt ctttttgtag
     1201 tattttccat gatcatgcct taggctttcg ccttcttttt ctctttcgcc tgaggtttgg
     1261 tctctgcttt cttcttttct tttgctggct tcccggtagg agctttctgc tctgccttgc
     1321 caagcttgcg cagattcaaa acggtcaacg ctcctgcgat cgtgcgtctg aggttacgaa
     1381 tctgagatgg attctggagc gaccgcgcca ctccaaattg aaaacgcagt tcgcgaattt
     1441 caacctgcga atcgtctacc agcttgtgca attctgcatc agccagttca tgtaatgcgc
     1501 gtgccattag atgagctcct ttttcacaaa tactgtgcgg attggaagtt tgtgagctgc
     1561 cagatccagc gcttctcttg cgagagcttc ttcaacgccg ctgatttcaa aaagaacgcg
     1621 gcctgcatag ataacagata cccagaattc aggattccct ttaccgctac ccatacgagt
     1681 ttcagcgggt ttacgagaaa caggagtgtc agggaagatg cgaatccaca gctttccacc
     1741 gcgcttcact ttacgtgtca tcgcgcggcg agccgcttcg atctgacgcg cagtcaagcg
     1801 accggaatcg agagccttca gtccgtattc accgaaggag acattgctcc cgcgctgaga
     1861 aagacctcta aggcggcctc tctgtctttt gcgatatttt actctttggg gcgccagcat
     1921 attctattcc gttaacccgt ttttcttctt actgcgtaat catcgtcttc ggctttttca
     1981 agacctggaa gtgcgtcacc atgataggtc caaaccttca caccgataat tccataagtg
     2041 gtgcgtgctt ctgcgtgagc atagtccaca cgcgcacgaa gagtatgaag aggaacgcgt
     2101 ccatctttgt actgttcctg gcgggccatg tccgcgccat tcaagcgacc ggataccatg
     2161 atcttcacgc cttccactcc accacgcatt gcgccgcgaa gtgcttgttt catgactctt
     2221 ctaaatggca ttctctgctc aagctgctgt gctatagact cgcctacggt ctgtgcaatc
     2281 gtttcaggct ttttcacttc cacgatgctg atattgagag gcttcgtgat tttcttacga
     2341 acttcagcct tcagcgcttc aatagaagct cccttcggcc caatgattgt ccctggacgc
     2401 actgtgtgaa tgttgatccc gattttctca gggaaacgtt cgatgacgat cttcacaacg
     2461 cctgcgcgct tgaatttctt ctgcaccatt ttgcggatca tcagatcctc atgcagattc
     2521 tgcctgtatg attcctttgc gaaccagatc gaatcccagg tccgattgat tcctacgcga
     2581 aggccgactg gatttacttt ctgacccatt gattcctcac tcgttactca gtacgatcgt
     2641 tatatgactc atgcgcttga gtcttctcat tccacggcca cgtgcacgag gtctgaagcg
     2701 cttgagagtt ggacccatgt ccacatacac tttcttaata tagagatctt cttcgcgcag
     2761 atccttctgc agagaaactg cgttggaacg cgctgttttc agtacgctct cgatgtagcg
     2821 agaacctttg cgcgggatga agcgcaaaat gtccattgct tccggatagc tgtatccgcg
     2881 aatctcgtct gcaaccagac ggattttacg aggcgcggag agtaggaact tacctgtgga
     2941 tttcgtttcc attcttatgc ccctggtttc gccttgattt tcttgtcgag gctggtgtgt
     3001 ccgcgaaacg tacgggtcgg agcgaattcg cccagtctgt gacccaccat gttctcatgg
     3061 atgtaaacag gaatgaagct cttgccgttg tacaaaagga tcgtgtgacc gatcatttct
     3121 ggaaacactg tagagcgtct ggaccaggat ttgaacggct tcttctgatt ctcgctgttc
     3181 agcttcgtga tcttcgccat caagtgctcg gcgatgtatg gccctttttt aagagatcta
     3241 gacattattc accaatcctc ttgttcttcc gacgctgcac aatgaaacga tcggatgtct
     3301 tgcgtttctt tcttgtcttg tagccgcgcg ttggttgacc ccatgggctg acaggatgac
     3361 gaccaccgga cgttttacct tcaccaccac catgtgggtg atccacaggg ttcataacca
     3421 caccgcggac ttttggtctg cgtccaagcc agcgcgcacg ccctgctttt ccgagactca
     3481 ccaggttgtg gtctttgttt ccaacgattc caacggtagc gttgcacgct tcgtggatct
     3541 tgcgaacctc cccggaagga agcttgatga tgatgtattc ctgatcacga cccgatacgg
     3601 ttgcaaacgc acccgcggat ctggcgatct gaccgccgcg tcctgcgcga agctcgatgt
     3661 tgtgaatgtt ggttcctggt gggatctcgc caagtggcag agagtttcca ggacgaatgg
     3721 gtgcgccttt tcctgcaacc actggttgtc ccactttcaa tccatccgga ctgagaatgt
     3781 agcgagcttc cccgtctgga tactggatca gcgcgatatt tgccgagcgg tttggatcat
     3841 actcgatgga ctttacaacg ccctgaatgt cgagcttgtt gcgcttgaaa tcgatgatgc
     3901 gatacatacg cttgtgacgt ccgcctttgc ggcgcactgc gatggatcca gagtttgcgc
     3961 gacctgctct ataatgaacg gtttcaagaa gagatttgcg cggctctact ttgctcagtt
     4021 cagaaaaatc aagaactgtc tggtagcgaa gcgtctttgt tactggttta aaacgtttaa
     4081 ctggcatatt ctatttcccg ttatgctgtt ctggcgaagt caatcttctg accgccctcg
     4141 agaacaacga tggccttctt ccacgatggc atccgtgttt tgccctggcg aaagcgtttg
     4201 agctttccac ggacattgat gattttcaca tccactgcct tcactttgaa gagctggtgg
     4261 agagcctgac gcacgagttc tttgtttgca tcaggatgaa tgcgaagaac gtaacgctct
     4321 ccttctttcc cgatgttctt gatcgcctca gatttctctg tgatgtgcgg acgctggatg
     4381 acagtattca gattcatgcc ggtttccttt tacgcttcag agtgtaatgc gcttcgaggt
     4441 acttgagcgc cccttcagag aagaagagga aatcagaata ataaagctca ggtgcattca
     4501 ggcgctgcgc atgcatgatc ttcgcatttg gcagatttcc gaaggacttc tgaagcttga
     4561 gatcagatac atccgttaca tatacaacag tagaatctgc aaatccggct ttcttgaaga
     4621 tgctcacgat tgcctttgtg ctgaactcag gcgcctgcag cgcgtcaatg attccaactt
     4681 cttcgcgctg cgcttttttt gccaggatag aaacaattcc gagcctgcga aggcgatcat
     4741 tcagaccaat gcgatagtcg cgcgggtttg gaccaaatac gatcccacct tttctccact
     4801 gaggagcgcg aatagaaccc tgacgagcat ggcctgtgcc tttttgacgc catggctttt
     4861 taccgccgcc gcttacttcc gcgaagtcct tggtcttgtg tgttccatga cgacgattgc
     4921 ggttttcagt acgaattaca gagtgaatgg cagcttcgct tacaggagca gcaaagatcg
     4981 catccgcgag ctcacccgtt cctgattgtg cgccctgcgc tgtgaacttc ttcactttca
     5041 tttggttacc tcgatacgca gaagggctgt gcgtgcacct ggcactgcac cctttacgaa
     5101 taacaggttg ttttcagcat ccactgcgac gattttcacg ttgcgcagag tgatctgatc
     5161 cccgccagtg cgacctggca tgcgtttgcc tctccacact tcagccggga atgtgtttgc
     5221 gccaagggaa cctggcgcac gatgaaattt agaaccgtga gcggccggac caccaccgaa
     5281 gccatgacgc ttcatagagc cctggaagcc tttgccttta gaaatgccag tcactcgaac
     5341 ggtgtcgcct gcattgaaga cagaaacatt gatctcttga ccggattgtc cttccatatc
     5401 gcggaattct gctagaattc tctgcggtgc gatgccggcc tttttaaaat gaccgatctc
     5461 ccctttggaa aggagctttt cacgaagagc atcaaatccg atttgaacag ccgcgtagcc
     5521 gtccttttcc accgttttta cttgcgaaac gacgcaggga cctgctcgca ggactgaaac
     5581 cggaaacagt ttcccggtct cgtcgtatat ctgcgtcatc ccgatctttt ttgccagcag
     5641 gcccgttgcc ataatcagct cttaatgtcc acactgactc cggcagggag ctgaagcttc
     5701 atcagcgcat ctaccgtatc agggtttgta ttcataatat caatgagccg cttgtaagtg
     5761 cggatctcaa attgttcgcg tgatttcttg ttcacgtgaa cggaacgaag tacggtgaac
     5821 ttctcggtcc gcgtaggcag cggaatcggc ccggagatct ctgctcccgt gcgacgcgcg
     5881 gttgccacga tctccgcagc tgagcgatcg atcaacttgt gatcgaacgc cttgagtttt
     5941 actctaattc tttgtgccat ggaccgattc ccgttcctta ctcactaatc tcagctacaa
     6001 cgccggatcc aactgtgcgg ccgccttcgc ggatcgcaaa cttgagtcct ttgtccattg
     6061 caatcggagt gatgagctca cagtcgattg atacgttatc acctggcatt accatttcgc
     6121 gtccttttgg aagaacgatg gttccagtta cgtctgtagt tctgaagtag aactgaggac
     6181 gatagttgtt gaagaatgga gtgtgacgtc cgccttcttc ttttgtaaga acgtagatct
     6241 ctgccttgaa ctttttgtga ggagtgatgg accctggctt tgcaaccacc tggccgcgct
     6301 ccacgtcttc tttcttgatg ccacggagaa gaagaccaac gttgtctccc gcctgaccgt
     6361 gatcgagaag cttacggaac atttcaatac cggttacaac tgtcttaaca gtgtctttga
     6421 taccgacgat ttcaacttct tcgttcacag agattttgcc ctgctccacg cgacctgtta
     6481 ctacagtacc acgaccggta atggtgaaca cgtcttctac tggcatcagg aagtctttat
     6541 ccagaacgcg ctttggatct ggaacataag tgtcgagagt ttcaaccagt ttgatgattg
     6601 cctgagttcc aagctcgctg tcttcgcctt cgagagcctt gaggcctgaa ccgcgaacga
     6661 aaggagtttc agcaggatag ccgtacttaa cgagcagttc cttcacttct tcctgaacga
     6721 gttcaaccat ttcttcacgt tcgccttcag cgatcatgtc tactttgttg aggaatacaa
     6781 cgatgtaagg aacaccaacc tgacgagcga gcaggatgtg ctcgcgagtc tgaggcattg
     6841 caccgtcagt tgcggaaaca acgagaatcg ccgcgtccat ctgagctgca cccgtgatca
     6901 tgttcttcac atagtcagca tgacctggac agtctacgtg cgcgtagtgg cgcttttctg
     6961 attcgtattc ctggtgagaa gtggcaatgg tgatcccacg tgctttttct tctggtgcgt
     7021 tatcgatttg atcgtacgca acagctttgt tcttaccgcc gaacttcttt gcaagtacag
     7081 tagtaattgc tgccgtcagt gttgtcttgc cgtggtcaac gtggccgatt gtgccaacgt
     7141 ttacgtgtgt ttttgaccta tcaaatttct cttttgccat gaccttctcc tatcttatcc
     7201 tttgtagtat tcctttagaa aagtcggaag gccagatttt caaaatcggg cccagccgtc
     7261 tattgatttt tgcgcttaaa ccgccgcctt cgccatgatt tcctgcgcta cgctgtttgg
     7321 tacttcttcg tagtgcttga actgcatagt ataggccgca cgaccctggg ttgcactccg
     7381 tagatctgtc gcataaccga acatttcggc cagcggcaca tctgcctgca ctacgcgggc
     7441 atttccgcgc tgctccatag cattgatccg accgcgtctg cggttcaggt cacctactac
     7501 ttcgcccatg tagtcttctg gagttacaac ttcgacgctc atgactggtt cgagcaagac
     7561 cggctttgcc ttacggcagg cttctttgaa gcccatggaa gctgcgatct tgaacgccat
     7621 ttcagaagag tccacatcat ggtagcttcc gtcgaatact tcgactttca cgtctaccat
     7681 tggatagccg gccaggacgc cgctctgcag cgcttcgtta cagcctttgt cgatggctgg
     7741 aatgtattcg cgaggaactg ttccaccgac caccttgttt acgaattcgt aacctttgcc
     7801 gggttcgttt ggcatcacac gcagccagca atgaccgtat tgaccacgac cacctgtctg
     7861 acggatgtat ttgccttctt cttctgcctg ctggcgaatc gt
//
LOCUS       DAOQJL010000120         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4989_length_7893_cov_4.867823, whole genome shotgun sequence.
ACCESSION   DAOQJL010000120 DAOQJL010000000
VERSION     DAOQJL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_7893_cov_4.867823"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLG78_07125"
     CDS             <1..457
                     /locus_tag="PLG78_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE22920.1"
                     /translation="HIVEEYRADGGLLLDAIQGLNPIQMNTRPVADTWTIQEIVVHMF
                     DSELIAVHRLRRLIAEQNPLLIGYDESAFARSLFYEKTDPVLAASMVKNLREMMADTL
                     NLCPPEAFEKVGVHSETGRIQVADYLKKNVEHLRHHMKFLNEKKKMVQR"
     gene            complement(459..1457)
                     /locus_tag="PLG78_07130"
     CDS             complement(459..1457)
                     /locus_tag="PLG78_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE22921.1"
                     /translation="MKQVSTVLLGLGRIAWMLEKDRLRYHPCTHAGSLKNPGMRKNPF
                     HLVGACDLIQERLYDFSSYWKKPLLLSRKPSEVLALRPDLAVIAASLPSHFELVRAAQ
                     KSGVHSIVLEKPPAANLKEATRLLTLCDQTNVWVNFERRYHPGYRRVKDIIDSKVLGE
                     MRSIRGRVLVGGSPGAGEAGPLLHDAIHWIDLLLWFAGKPARAFARMMRNTPDAGEHT
                     AFATFEFSDFNAVLESGGRRKYFEFEMEIDFAAGRIYCGNNGFRMEVSKPSRKYSRFN
                     ELRTAKPLLKKTQNPWQNLYQEVASVSSGETTTIHSSLASAVEAMKVIQMLEKNQR"
     gene            complement(1454..1738)
                     /locus_tag="PLG78_07135"
     CDS             complement(1454..1738)
                     /locus_tag="PLG78_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22922.1"
                     /translation="MTFLLEKDFRIGFFAGCALHLWYLIPRVSWLSLKCGARCSDIAT
                     ADAPISLLYLAFPDPGVIFFSTILGTIEWGVWFYLILKAVRFLVSRFLPS"
     gene            1747..3507
                     /gene="mutL"
                     /locus_tag="PLG78_07140"
     CDS             1747..3507
                     /gene="mutL"
                     /locus_tag="PLG78_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNE22923.1"
                     /translation="MAEKQIRRLPSIVIDRIAAGEVIESPASVIKELSENSLDAGATR
                     LRIETTAGGMDSLVVEDDGSGIPFHDLPAAIERHATSKIGSIEDIENILSFGFRGEAL
                     ASIASVSLLRLTSRTRNEEIGGILESRAGTISLHERKACSYGTVVQVRDLFFATPARK
                     KFIKSEKTENLKIYREIRKIALSSPNVEILYIRDGQEYLHFARRNTLRERLADVFPSD
                     ALDRWIEIHAESDGISVFGFISSPDAVRANRDNQLTFVNGRWVELKYMSYLLKKGYGD
                     LIAHGMHPSAVIHFTIQPDMIDVNVHPAKREIRFLDENRLQGLVIRAIQRALSAGPVV
                     FSEQTLQRNFREVSSNPLSVESLSPESSFLIEPARGAVLVSLSNQQAALPEFIRPPEK
                     PSVQIPSRLRQLGVVFGTYILAEAEDGLYLIDQHTAHERVNYEKKKKQLASIQGQRQP
                     LLHAAVIRLQPDEMQVIQTHEQELLRVGFVVEAMGKDACAVREVPLFMDPGEESDTLK
                     HTIQRVLDGEESIQIFDEYAAMKACKASIKKNDVIGDHVLADILKDLMQCDDPTRCPH
                     GRPTMVRISRDAMDRLFMRS"
     gene            3512..4030
                     /locus_tag="PLG78_07145"
     CDS             3512..4030
                     /locus_tag="PLG78_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNE22924.1"
                     /translation="MKSVVIGVTGSIAAYRTCDLIRSLTKKGVSVHVCMTESAEHFVG
                     RITFEALTGQRVYTSQWESGMPHIELKRLSKLLAIVPATADIIGKFASGIADDLLSST
                     YLAHTGTVLIAPSMNPGMYASKAVQRNLQRLREDGVILVDPANGEAVCGDEGQGKLAT
                     IEQIESRILELI"
     gene            4027..4683
                     /locus_tag="PLG78_07150"
     CDS             4027..4683
                     /locus_tag="PLG78_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNE22925.1"
                     /translation="MISRKLIVTSGPTREWLDPVRYISNPSSGRMGWSIAKAGVGVFK
                     SVVYIAGPGSEAFRRVEGAHNVEVDTTENMLSAVLEALEKNCVLIMAAAPADYAPVEF
                     ASAKIKKDPARNLNVEFRPTPDILMEVGANAPDGMLRIAFAAETDHVRENALIKMQKK
                     KVDFICANQVFKDGMGFATESGSLLILDRNGAEKKLGPAPKDALGQELVSFVAEHLNS
                     "
     gene            4680..4898
                     /locus_tag="PLG78_07155"
     CDS             4680..4898
                     /locus_tag="PLG78_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22926.1"
                     /translation="MKIKIYSLHLGYREADAKIEKDVKGRHVLVVDGVGPVSALDYIK
                     QGMKLLSASEQEIAVLKQAGYPMKNELI"
     gene            complement(4895..6166)
                     /locus_tag="PLG78_07160"
     CDS             complement(4895..6166)
                     /locus_tag="PLG78_07160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22927.1"
                     /translation="MRTLSPFQIETLRSEMLRTTILAVFFLSGALFFTVFSLTFPAEF
                     NQVFQGTLQWWLPPIFFSIVATYLLGVRTLAAHFLKTSRPYPTIGRYMNAFLETSIPT
                     LVLIFMMQFRNPFYVLLMPPVYVYFIFIILSALRVDRLLSIFTGLVAALEYGALSVYA
                     LLYAEPVGMDPALAAPGGFLARFLFLAAAGITTGFVTRQIKHQLTASFEAGRERDKIA
                     DLFGQHVSPQVVDRLLSQNTEWLSESRHVCMMFLDIRDFTRFSQNREPEQVVNYLNSL
                     FDFMIEMINEHQGYINKFLGDGFMAVFGAPISSGADVQNAVAAALKIVERVDSDSASG
                     IIAPTRVGVGIHAGIAVTGHVGTTRKKEYTIIGDVVNLASRIEQLNKEFGSKILVSED
                     VWKELKDHKGEELGTVTVKGRDEAVRIFKLA"
     gene            complement(6163..7422)
                     /gene="serS"
                     /locus_tag="PLG78_07165"
     CDS             complement(6163..7422)
                     /gene="serS"
                     /locus_tag="PLG78_07165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNE22928.1"
                     /translation="MLDLKRIQDNPEELESMLAKRRFDSASLTGLKDLIKTVKTLKAD
                     ADKIRAERNSASKEIGNLIGQGKKDQAEKRKAEVKDLGDKIADLEKKCEEQEESLSDI
                     VLGLPNFIEPEVPIGDETANKTVREIGHKPQFDFKAQTHFDIGEKLGIFDFERGVKLA
                     GSRFYTYRGLGAKLERALMNFMLDLHTGEHGYQETWVPILVNDACMTTTGQYPKFKGE
                     YYRLDRDELSLIPTSEVPLINLYRDDILDEKDLPVAMTAHTSCFRREAGAAGKDTRGL
                     IRVHQFQKVELIQIVHPDQSREIHEKMVGHAEEVLKRLGLHYRVVLLSSVDMGATAAK
                     TYDLEVWMPGLDRYMEISSISNCMDFQARRGMIRFKDAAGKNKPKLAHTLNGSGVAIG
                     RALAAVIENYQKKDGTIEIPTVLQKYL"
     gene            complement(7435..>7893)
                     /locus_tag="PLG78_07170"
     CDS             complement(7435..>7893)
                     /locus_tag="PLG78_07170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNE22929.1"
                     /translation="ELSVELQLPIIVHLRDSQKFEPGKIQSVDDAEAMVRETKARGVL
                     HCFTYGYEEAMRFVDMGWFISFSGILTYKNAQVIQDAAARLPLECILVETDAPFLTPN
                     PVRGKPNQPAYTVHTAKFLADLRSKANGEDPDQVLAQIHQNSERFFALKK"
BASE COUNT         1973 a         1841 c         2042 g         2037 t
ORIGIN      
        1 acatattgtt gaggaatata gagcagatgg tggtctgttg ctggacgcaa tccagggtct
       61 caatccaatt caaatgaaca caaggccagt ggcggataca tggacgattc aggagatcgt
      121 ggtccacatg ttcgacagtg aactcatcgc ggtgcatcgg ctgcgcagac tcattgcaga
      181 gcaaaatcca ctgctcatcg ggtatgatga aagcgctttt gccaggtctc tcttctacga
      241 gaagacagat ccggttctgg ccgcgtcgat ggtaaagaat ctaagggaaa tgatggcgga
      301 cacgctcaac ctctgccctc ccgaggcatt cgagaaggtg ggtgtgcaca gcgaaaccgg
      361 tcggatccag gtggcggact atctcaaaaa gaatgtggaa cacctgcgtc atcatatgaa
      421 gttcttgaat gaaaaaaaga agatggtgca gcgctagatc aacgctggtt cttttcgagc
      481 atctggatga ctttcatcgc ttccactgcg gaagccaggc tgctgtggat ggtggtggtc
      541 tctccgctgg aaacggaagc cacttcctga tatagattct gccacgggtt ctgggtcttc
      601 ttgagcagcg gttttgcagt tcttagttcg ttgaacctgg aatattttct agatggtttt
      661 gagacttcca ttcgaaagcc attgttgccg cagtaaattc ttcctgctgc gaaatcgatt
      721 tccatctcga attcgaagta ctttcgtctt ccgcctgatt caagaactgc gttaaaatca
      781 gaaaactcaa aagtggcaaa cgcagtgtgt tctcctgcat ccggagtgtt tcgcatcatg
      841 cgagcgaacg ctcgcgcagg ctttccggca aaccacagga gcaaatcgat ccaatgaatg
      901 gcgtcatgca atagcggccc cgcttctccc gctccgggag agccaccaac aagaactctg
      961 cctcgaatgc ttcgcatttc ccccaggact tttgaatcga tgatgtcttt tactcgtctg
     1021 taccctggat ggtatctcct ttcaaagttc acccacacat ttgtttgatc gcataacgtt
     1081 aaaagacgag tcgcctcttt tagatttgcg gcaggaggtt tttcgagtac gatggagtgc
     1141 acccctgatt tttgagcagc gcgcaccaat tcaaagtgag atggaaggct tgctgcaatc
     1201 actgcaagat ctggccgcaa cgccagcacc tccgaaggct ttcgggatag gagcaaaggc
     1261 tttttccagt acgaggaaaa atcgtataat cgctcctgga taaggtcgca ggcacccacg
     1321 agatggaacg gattttttcg cattccagga tttttcaggg atcctgcatg agtgcagggg
     1381 tgatatcgta ggcggtcttt ttccagcatc caggcaattc tgcccagacc cagaagcact
     1441 gtggagacct gcttcatgac ggaaggaatc tggatacgag gaaacgaact gctttaagaa
     1501 tcagatagaa ccacacgccc cattctatgg ttccaagaat ggtggagaag aagatcactc
     1561 cgggatcagg aaatgcaaga tatagaagac tgatgggagc atctgctgtc gcaatgtcgg
     1621 aacagcgcgc tccgcatttc agggaaagcc agcttacgcg cggaatgaga taccagaggt
     1681 ggagtgcgca cccggcgaaa aagcctatac gaaaatcctt ttcgaggagg aaagtcatag
     1741 tggactatgg cagaaaagca gatacgcagg ctcccgtcta tagtaatcga tcgcattgca
     1801 gcaggcgagg tgatcgagag tcctgcctcc gtgatcaaag aactctccga gaactctctg
     1861 gatgcgggtg ccaccaggct tagaatcgaa acgacggctg gcgggatgga tagcctggtc
     1921 gtagaggatg atggatccgg tattcccttt catgatttgc ccgccgccat cgagaggcat
     1981 gcaacgtcca aaattggatc tatcgaagac atagaaaata tattgagctt tgggtttaga
     2041 ggagaggctc tcgcgtcgat tgcctctgtc agtctactcc ggctcacaag tagaaccaga
     2101 aatgaagaaa taggagggat tcttgagagc cgggcgggaa ctatttccct gcacgagagg
     2161 aaagcctgtt cctatggcac tgtggttcag gtgcgtgatc tattctttgc aacaccggca
     2221 cgaaagaaat ttatcaaatc ggagaaaacg gaaaacctca aaatctatcg tgagatccga
     2281 aaaatcgctc tttcgtcgcc taacgttgaa atcctgtaca tccgcgacgg tcaggaatat
     2341 ctgcacttcg caagaaggaa tactcttcgc gaaagattgg cagacgtatt tccctcggat
     2401 gcgctagatc gctggattga aatccatgca gaatcagatg gaatttctgt tttcggtttt
     2461 atcagttcgc cagatgcggt ccgcgcgaat cgagacaacc agctcacttt tgtaaatggc
     2521 cgatgggtgg agctgaaata catgtcttat ctactaaaga aggggtacgg cgatctcatc
     2581 gcgcacggga tgcatccatc tgccgtgatt cacttcacca tacagccaga catgattgat
     2641 gtgaatgttc accctgcgaa aagagagatt cgatttctgg atgaaaacag attgcagggt
     2701 cttgtgattc gcgccattca gagagcactg tctgccgggc ctgtcgtgtt ttctgaacag
     2761 actttgcaga gaaactttcg tgaggtgtcg tcgaatcctc tgtcagttga gtctctttcg
     2821 cctgagtctt ctttcttaat tgagccagcg cgcggcgcag ttctcgtatc gctatcgaac
     2881 caacaagcgg cccttcctga attcatacga cctcccgaaa agccttcggt tcaaatacct
     2941 tctcgactga ggcaactggg agttgtgttc ggaacctaca ttctagcaga agcagaagat
     3001 gggctttact tgatcgacca gcacactgcg catgagagag tgaactacga gaagaaaaag
     3061 aagcagcttg cgtccattca gggccagagg cagcccttac ttcatgcggc agtgatccga
     3121 cttcagccgg acgaaatgca agtgatccag acgcacgaac aagagcttct aagagtcggt
     3181 ttcgtcgtgg aggccatggg aaaggatgca tgcgctgtgc gggaagtgcc ccttttcatg
     3241 gacccgggag aagaaagtga tacgctcaag cataccatcc agagagtact cgacggagaa
     3301 gaatccattc agatattcga cgaatacgcg gcgatgaagg cgtgtaaggc ttcaattaag
     3361 aagaatgacg tcataggcga tcatgtcctg gccgatattc taaaggattt gatgcaatgc
     3421 gatgatccga ctcgctgccc tcacggaagg ccaaccatgg tgcgaatttc gcgcgatgca
     3481 atggaccgat tatttatgag aagttagatt tatgaaatca gtcgtcattg gagttacagg
     3541 aagcattgcc gcctatcgta cgtgcgattt gattcgctcg ctcacaaaga agggtgtatc
     3601 tgttcacgtg tgcatgacgg aaagcgcaga acatttcgta ggaagaatta cttttgaagc
     3661 gttgaccggg caacgagtgt atacgagcca atgggaaagc ggcatgccgc acattgaact
     3721 caagcgtctt tctaaacttc tggcaattgt tccggcaacc gcggacatca ttggaaaatt
     3781 cgcttcgggt attgcagacg atttgctcag ttcaacctat ctcgcgcaca ccggaaccgt
     3841 gctgatcgcg ccctctatga atcctggaat gtatgcgagc aaagcagtgc aaaggaatct
     3901 gcagaggtta cgtgaagatg gcgtaatact ggtagatcct gccaacggag aagcagtgtg
     3961 tggagacgaa gggcagggaa aacttgcaac cattgaacaa attgaatcaa gaattctgga
     4021 gttgatttga tttcacgtaa gctcattgta acttctggcc caacccggga gtggctcgat
     4081 ccagttcgat atatttccaa tccttccagt ggtcgtatgg gctggtcgat tgcgaaagcg
     4141 ggcgtcggag tttttaagtc cgtggtttat attgccggcc ccggaagcga agcctttcgg
     4201 cgtgtagaag gcgcgcacaa tgtggaagtg gatacaacgg agaatatgct gtcggcggtt
     4261 ctcgaagctc tggaaaagaa ttgcgttctc atcatggcgg cggctcccgc agattatgct
     4321 cccgtcgaat tcgcttctgc gaaaatcaag aaggatccag cgcgaaatct gaacgtagag
     4381 tttcgtccta ctcccgatat tctcatggaa gtaggcgcca atgcaccaga cggaatgctt
     4441 cgaatcgcct ttgctgcaga gacagatcat gtacgcgaaa acgctttgat caaaatgcaa
     4501 aaaaagaaag tcgatttcat ctgtgccaat caagtattca aagatggcat gggtttcgcg
     4561 acagagtccg gctctctttt gattctggat agaaacggtg ctgaaaaaaa acttggacct
     4621 gccccgaaag atgctctggg tcaggaactc gtttcgtttg tagcggagca tctgaattca
     4681 tgaaaatcaa gatttattca ttgcatcttg gatatcgcga agcggatgcg aagattgaaa
     4741 aagatgttaa ggggcgtcac gttctggttg tggatggggt gggccccgtg agtgctctgg
     4801 actatatcaa acagggaatg aagttgcttt cagcttccga gcaggagatt gctgttctca
     4861 agcaggccgg ttatcccatg aagaacgaac tgatctaggc cagtttaaaa atgcgaactg
     4921 cttcatcgcg gcctttcaca gttacagtac ccagctcttc tcccttatga tcctttagtt
     4981 ctttccacac gtcttcggac accaggatct tacttccaaa ctctttgttc aattgctcta
     5041 tacggctggc caggttcacc acatctccaa tgatcgtgta ttctttcttg cgcgtggttc
     5101 ctacatgacc cgtaactgct atgcctgcat gaattccaac gcccactcga gtgggcgcta
     5161 tgattccgga ggctgagtcc gaatccacgc gctctacaat ctttagcgcg gccgcaaccg
     5221 cgttttgaac atctgcgccg gatgaaatcg gcgcgccaaa gacagccatg aagccatcgc
     5281 ctagaaactt attgatgtaa ccctgatgct cgttgatcat ttcaatcata aagtcgaata
     5341 gactgttaag atagtttacc acttgttctg gttctcgatt ttgtgaaaac ctggtgaagt
     5401 ccctgatatc caggaacatc atgcacacat gcctggactc gctgagccac tctgtgtttt
     5461 ggctcagcag tcgatctacc acctgtggtg atacatgttg tccaaacaga tctgcgattt
     5521 tgtcgcgctc tctgcctgcc tcaaatgaag cggtaagctg atgcttgatc tgtctggtta
     5581 caaatccagt ggtgattcct gctgccgcaa gaaacaaaaa gcgtgccaga aatcctcctg
     5641 gggctgccag ggccggatcc attcctacag gttcagcgta taacaatgca taaacggaaa
     5701 gggctccgta ttccagggct gcgacgagac ctgtaaaaat agatagaagt ctgtctactc
     5761 tgagtgcgga taaaatgatg aaaataaaat acacatatac gggtggcatc aagagaacat
     5821 agaacggatt gcgaaattgc atcatgaaga tgagaacgag agtgggaatg cttgtttcca
     5881 ggaacgcatt catgtatctc ccaatcgtgg gatacggtcg ggaagttttc aagaaatggg
     5941 cagccagagt acgcactccg agtaggtacg tggctacgat ggagaagaag atgggaggaa
     6001 gccaccactg tagagttccc tgaaacactt ggttgaattc agcgggaaaa gtgagcgaga
     6061 agaccgtaaa gaagagagcg cctgacagga agaagacggc gagaattgtg gtgcgcagca
     6121 tttcgctgcg cagagtttct atttgaaaag ggctgagggt tctcacaggt acttctgaag
     6181 tactgtggga atctcaatcg tgccatcctt tttctgataa ttttcgatca cggcagcgag
     6241 cgctctgcca atggcgactc cagacccgtt caatgtgtgt gcaagctttg gtttgttttt
     6301 tccagccgcg tctttgaatc gaatcattcc cctgcgagcc tgaaagtcca tgcagttgga
     6361 gatagatgaa atttccatgt agcgatcgag gccaggcatc cagacttcaa gatcgtacgt
     6421 tttggctgcg gttgcgccca tgtccacaga ggaaagtaga accacacgat agtggagtcc
     6481 aagtctcttc agcacttctt ccgcgtgtcc taccattttc tcgtgaattt ctcgcgactg
     6541 atcaggatga acgatctgaa tgagttccac tttctggaat tgatgcactc tgatgagtcc
     6601 gcgagtgtct tttccggcag caccggcttc tctgcggaag caggaagtgt gggctgtcat
     6661 ggcaacgggc aggtcctttt catcgagaat atcatcgcga tagagattga tgagaggtac
     6721 ttcggatgtt gggatcagag agagctcatc gcggtccaat cgatagtatt cacctttgaa
     6781 ctttggatac tgaccggtcg tcgtcatgca cgcatcattg actagaatgg gaacccacgt
     6841 ttcttgatat ccgtgttccc ctgtgtgaag gtcgagcatg aagttcatca atgcgcgttc
     6901 cagtttagcg cccagtccgc ggtacgtata gaatctggat cctgccagtt ttactccgcg
     6961 ctcaaaatca aagattccga gtttttctcc gatgtcgaag tgcgtctgcg ctttgaaatc
     7021 aaactgaggc ttatgcccga tttcgcgtac tgtcttgttt gcagtttcgt ctccgatcgg
     7081 tacttcgggt tctatgaagt tgggtagacc aagaacgatg tcactcaggc tctcttcctg
     7141 ttcttcgcat ttcttttcca gatctgcgat tttgtctccg aggtctttga cttccgcctt
     7201 tcttttctcc gcctggtctt tctttccctg gccgatcaga tttccgattt ccttgctcgc
     7261 ggaatttctt tccgcgcgga ttttgtctgc atctgccttg agagttttta ccgttttgat
     7321 aagatccttg aggcctgtga gacttgcaga atcgaatcgg cgttttgcaa gcatggattc
     7381 aagttcttct ggattgtcct ggattctttt gagatcgagc atggttgtgt tcctttactt
     7441 tttaagcgca aaaaagcgtt ctgagttttg atggatttga gcaagcacct ggtccgggtc
     7501 ttctccgttt gcttttgagc ggagatctgc cagaaatttt gcagtgtgca ccgtgtatgc
     7561 gggctggttt ggttttccac gaaccggatt cggagtgagg aagggtgcat ctgtttctac
     7621 caggatgcat tcgagaggca gtcttgctgc ggcgtcctga atgacctgag catttttgta
     7681 ggtaagaatt ccgctgaatg aaatgaacca gcccatgtct acgaagcgca ttgcttcttc
     7741 gtagccatat gtgaagcagt gtagtacgcc tctggctttt gtctcgcgca ccattgcttc
     7801 tgcgtcatct acggactgta tttttcctgg ttcgaatttc tgggaatcac gcaaatggac
     7861 gataatcggc agctgcaatt ccacggacaa ttc
//
LOCUS       DAOQJL010000121         7884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_4999_length_7884_cov_4.386128, whole genome shotgun sequence.
ACCESSION   DAOQJL010000121 DAOQJL010000000
VERSION     DAOQJL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_7884_cov_4.386128"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLG78_07175"
     CDS             complement(<1..786)
                     /locus_tag="PLG78_07175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE22930.1"
                     /translation="MKRVLITGSRGFLGQHLCLSLLRDGYEVTGLDRSGKDRKPPSWW
                     TLYPDSKPDQIQQVESDLGSCDQIRGVLEQQSYDFILHLAGTAFVPEGWINTADVAVN
                     NTVVPIRLFQAARDANFKGRIVFVSSGEVYGAKHPMPLLEDFTLEPENPYAESKRSAE
                     QYAGYFVSRGLDIVIARPFNQIGPGQNPAFVIPSFLCRIRDAAARGETEIQVGDLDAE
                     RDFLDVRDAARAYSIVAEKGIKGQAYNICSGRPVTIRRVLDLAL"
     gene            complement(783..2075)
                     /locus_tag="PLG78_07180"
     CDS             complement(783..2075)
                     /locus_tag="PLG78_07180"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNE22931.1"
                     /translation="MKETAFFWFRRDLRLKDNSGLFHALSSGLAVIPVFIFDTNILEN
                     LPRDDARVTFIHSVLTRLKTFLESKGSTILVMHGDPLTVWKELIAKHKPRAIFANHDY
                     ESYAIERDQKISEICKSSGVDFKTTKDQCIFEKSEVVKDSGEPYTVFTPYFRKWKVRL
                     ESFDMTAFPSEKRLERMARLPASKMPGLDQLGFVPSSIRIEDPSIPENIIASYAETRD
                     YPSLRGTTHLGVHLRFGTLSIRTLAKSAQKSQVFLSELAWRDFFMMILYHFPHSATRS
                     FKPQYDRIVWRNNENEFEAWKEGRTGYPLVDAGMRELVQTGFMHNRVRMVTASFLCKH
                     LLIDWRKGERFFAEKLLDYDLSANVGNWQWACGSGCDAAPYFRIFNPSAQEKKFDPDR
                     AYIKQWIGELSSSYPEPIVKHELARDRCLKVFKEALAK"
     gene            complement(2072..2968)
                     /locus_tag="PLG78_07185"
     CDS             complement(2072..2968)
                     /locus_tag="PLG78_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNE22932.1"
                     /translation="MVIVITGGTGLLGKATARTLESGGHEVRILSRNASARFRWDPVH
                     AYIDKSVFEQADAVVHLAGETISGRWTAEKKRQIIESRSRSTDLLAEAISSTGLKPVV
                     ICASAIGYYGDRGKHVCMETDGPGSGFLCDTTRIWEERLADIPASRKVILRIGVVLSM
                     LGGALPQMVLPVKMFAGSPFGSGRQYISWIHIEDMVKMICCSIDRSMEGALREDLSGT
                     YNAVAPHPVINDDFMREIAATLSKPYFLPAVPSFLLKGMLGEMSVLLTDSIRVSANRI
                     QDAGFSFKFPDLKAALGSLLKK"
     gene            3067..4704
                     /locus_tag="PLG78_07190"
     CDS             3067..4704
                     /locus_tag="PLG78_07190"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNE22933.1"
                     /translation="MSKIGTQLLRAYEANGKDRSLTMPAFRPVLERPVTILSVLGLVL
                     AVPLYWVAPWASATAAALSVVLFGIREVFIQDDHAIVRIYGPMGRFRYIFENLIRDKY
                     LQYFNETNTDGRPIPRIVRDYIYQKAHHVKALSSFGTELDIFDTELSTGCRILHRNFP
                     AHVDGVSYQMSIGDGRPGVRPFVVKNAINISAMSYGAINNRAAEALSLGAKDVAYVNT
                     GEGGYGPHGAAGNDVVFQIGTGKFGVGEEAVLPDGTATRKLNKGLLQELVRNHPNIRM
                     IQIKISQGAKPGIGGHLPADKVTPEIADVRRVTPYKTVISPPQHAELRASSPKEAVSK
                     LIEFIEEIRKLTELPVGIKLCVGRLDELDLLVLAMKKTGKGPDAIQVDGADGGTGAAP
                     NLFVNYVGYGSSIETLQYLDKLLKKHRIRDRVRLSSSGRIFTPAHAAYAFASGADTCE
                     TARAAMLSIGCIQAQQCHTNKCPTGITTNSAWRMRGIHIPEKSTRVHHFLKGFHEDML
                     ELTRVMGHSDPRDIKLDDLRVMSRGGAFSAFFSDSHV"
     gene            4697..5713
                     /locus_tag="PLG78_07195"
     CDS             4697..5713
                     /locus_tag="PLG78_07195"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE22934.1"
                     /translation="MFNRVIRIAGWLGINTVLGTMNGLLISFYSPEPFARVFLTAQIM
                     THVLCLLIEFAMFTAGIQLYRIGTHKKQPVRYFTAALCITAISAVCGVLAGGRIHQEL
                     LGFNPYENSRFYNVIFGSIIFAIGLASIDRFFRYLRETRLLAERSLQEARLAVLQERM
                     RPHFLFNTLNSIHAMLPDHADRADAAILMLADSYRFLLGPAEKPLVSFMEEWEFCKNY
                     VELMKVRFGERLSVWMEHRGDFSRVLIPPLSIQPVIENAFRHGVQKIPGNAIVQISAE
                     CAGSSVRIEVKDNGPGLPSDFESRSIDNIRSRLRFHFPGADLEVSNLGESGVSSRITL
                     TGAA"
     gene            5713..6411
                     /locus_tag="PLG78_07200"
     CDS             5713..6411
                     /locus_tag="PLG78_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNE22935.1"
                     /translation="MPLRCYVIEDEPPAREILFRFIEARKDLSLAGWAGDARSALNYL
                     KVNPADLVFLDVQLPGMSGFEMLEELRPSPAVIFTTAGQAHAVPAFDHGAVDFLLKPF
                     SMERFDIAVNRVVQKRTTNPSSGGLAVTEGENHYLIPFGEIIYLSSRGKATAVHTTDR
                     DFESQRILKDIESRLPESFLRIHKQYILNLDFLAHLKYRVGGGYTATLRDSDETSVPV
                     GRGFAGALRARWSG"
     gene            complement(6412..>7884)
                     /locus_tag="PLG78_07205"
     CDS             complement(6412..>7884)
                     /locus_tag="PLG78_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE22936.1"
                     /translation="RMDRYVSRGIGEVPAYVADFVPRAFHVIRRFKPPEDFFREITAI
                     ADLQDQSGIPFKDPDGMIRTYSENANKIRAFLKRLTQDGFRTVIPLTFKSELLGYMVG
                     GASADSQMRYSDKNLLEAFRFSAALAVRNAMLYDELSLLKGKAEESVQQLSEYIVDMH
                     NAREHKMSEKTFVYLSKSMNDLVDRTRNLAGKNQPMLILGETGTGKEMVANLIHDASM
                     PESPFVAVNCAAIPGNLWESEVFGHVKGAFTDAKTDRPGKIEQAAGGTIFFDEIGEMP
                     LEMQPKMLRLIQERVFQRVGGTKEIKTDCRFIFATNRNLEEMQAKGTFRSDLYYRINV
                     FQIRIPPLRERRADIPALAQFFTEKYCAEMGYPARKLGDGAMDALCRFDWPGNVRELE
                     NAVIQAIVNSTTDIIRREDLPVQLLDARKMRSESPAEIQKPSPGNQWDGRPLEEILNE
                     HARRIIESALAKTKGNKVEAAKILGLKRATFYNRLKELGM"
BASE COUNT         1853 a         1949 c         2008 g         2074 t
ORIGIN      
        1 caatgcaaga tcgaggactc gccgaatggt aacaggtctt ccggagcata tattgtacgc
       61 ctggcctttg attccttttt ccgcaacaat cgagtaagcc cgggccgcgt cgcgcacatc
      121 caggaagtca cgctctgcgt ccagatcgcc tacctgaatt tcagtttcac ctctggctgc
      181 ggcgtcccgg atgcgacaga gaaaagaggg aattacaaaa gctggattct ggcctggtcc
      241 aatttgattg aatggtcgtg cgatgactat gtccagcccg cgcgaaacaa agtaccctgc
      301 gtattgctcc gcggatcgct tactctctgc atatggattt tctggctcga gagtaaaatc
      361 ttccagcaga ggcataggat gcttcgcgcc atacacttct cccgaactta caaacacaat
      421 tctgcctttg aagtttgcgt ctcgcgccgc ctgaaacaat cggatcggaa cgacagtgtt
      481 attgactgcg acgtctgcgg tattgatcca gccttctggc acaaaagcag tgccggccag
      541 atgaagaata aaatcgtacg attgctgttc gagtactcct cggatttgat cgcagctccc
      601 cagatctgac tctacctgtt gtatttgatc tggcttcgaa tccggataca gtgtccacca
      661 ggaaggcggc ttgcgatctt tgccagaacg atcgaggcca gtcacctcat agccatcgcg
      721 aagaagggaa aggcacaggt gctggcccag gaatcctctg cttcctgtga tgagtactcg
      781 tttcattttg ccagagcctc tttaaacact ttcagacagc gatctcttgc cagttcatgt
      841 ttcacaatcg gttccggata ggagctcgaa agctctccaa tccattgttt tatataggcc
      901 cggtcggggt cgaacttttt ctcctgcgct gacggattga aaatcctaaa gtaaggggca
      961 gcatcgcatc ctgagccgca tgcccattgc cagtttccaa cattcgcaga aagatcataa
     1021 tcgagcagtt tttctgcgaa gaaccgttcc ccttttctcc agtcgatgag taggtgtttg
     1081 caaagaaagc ttgcagtgac cattcgcaca cgattgtgca tgaaacccgt ttgtacgagc
     1141 tcgcgcattc ctgcgtctac cagagggtaa ccggttctcc cttctttcca tgcttcgaat
     1201 tcattctcat tgtttcgcca cacaatgcga tcatactgcg gtttgaagga tcgcgtcgcg
     1261 gagtgcggaa aatgatagag gatcatcatg aagaaatcgc gccaggcgag ctctgacaaa
     1321 aacacctgcg acttctgtgc gctctttgca agtgttcgaa tgctcagagt tccaaacctg
     1381 agatgcactc ccagatgtgt tgtgccgcga agtgaagggt agtcgcgcgt ttcggcataa
     1441 gatgcgatga tgttttccgg aatcgaagga tcttcgatgc gaatggagga aggaacaaat
     1501 ccgagctgat cgagacccgg catttttgat gcaggcagcc tggccattct ttcgagcctt
     1561 ttctccgatg gaaaggcagt catgtcaaac gattcgagcc gaaccttcca tttgcgaaag
     1621 tacggcgtaa acacagtgta gggttcaccc gagtctttca ctacttccga tttttcgaat
     1681 atgcattgat ctttcgttgt tttgaaatct acaccggatg atttgcatat ctctgaaatc
     1741 ttttgatctc tctctattgc ataagattcg tagtcgtgat ttgcgaagat agcgcgcggt
     1801 ttatgttttg cgatgagttc tttccataca gtgagtggat ctccgtgcat caccagaatc
     1861 gtgctgcctt tgctttccag aaaagtctta agtctcgtta gaacagaatg gatgaaggtc
     1921 acgcgcgcgt catcgcgtgg aagattttcg aggatgtttg tgtcaaagat aaatacagga
     1981 atgacggcca gaccactcga tagtgcatga aagagccccg agttgtcctt cagtcgcagg
     2041 tcgcggcgaa accagaagaa agcagtctcc ttcatttctt gagaagtgat ccaagcgcgg
     2101 ctttgagatc tggaaatttg aaactaaatc ctgcgtcttg tattctgttt gccgatacgc
     2161 gaatgctgtc cgttaagagg acagacattt ctccgagcat tcccttcagt aagaaagagg
     2221 gcacagcagg cagaaaataa ggcttcgaaa gagtggccgc gatttcgcgc atgaaatcgt
     2281 cattgatgac cgggtgagga gccaccgcat tgtatgtgcc ggatagatct tcgcgcaaag
     2341 ctccttccat ggagcgatct atgctgcaac agatcatttt gaccatgtct tctatatgga
     2401 tccaggaaat gtattgtctg ccggatccaa aaggcgatcc agcaaacatc ttcacgggca
     2461 gtaccatttg cggtaaggct ccgcctaaca ttgacaagac gactccgatt cgaagaatca
     2521 ctttgcgtga agcggggatg tccgcgagcc tttcttccca gattcgagtt gtatcgcaca
     2581 gaaacccact ccccgggccg tccgtttcca tgcaaacatg ctttcctctg tctccatagt
     2641 agccaatggc cgaagcgcaa atcacaacag gttttaagcc ggttgaggaa atggcttccg
     2701 ccaggagatc ggtgcttctg ctgcggctct ctatgatctg tcttttcttt tccgccgtcc
     2761 accttccgga gatcgtttct cctgcaagat gaaccacagc atctgcttgt tcaaaaaccg
     2821 atttgtctat gtaagcatgc actggatccc atctgaatct ggcgcttgcg tttcgagata
     2881 gaatgcgaac ctcgtgcccg ccgctttcca gcgttcgggc cgttgcctta cccaggagcc
     2941 ctgttccgcc ggtgatgacg atgaccatgc ccgcaaggat ccgggatgag cgcgcgaggc
     3001 aaagtgtttt tgcaaaatct attgcgggtc cgtcgtcatt cttcaggctg gggcatgccg
     3061 tacaccatgt cgaagatcgg aacccagctt ctgcgcgcgt atgaggccaa tgggaaagat
     3121 cgttctctta ccatgcctgc atttcgtccg gtgcttgaaa gaccagtcac aatcctttct
     3181 gtcctgggtt tggttttggc tgttccactt tactgggtag ctccgtgggc ttctgccact
     3241 gcagccgcac tttctgtggt gttgtttgga attcgggaag tcttcattca agacgatcac
     3301 gcgattgttc gcatttacgg acccatggga agatttcgat atattttcga aaatctcatt
     3361 cgcgacaaat acttacaata ctttaatgaa acgaacacag atggaagacc cattccgcgc
     3421 atcgtgcggg attacattta tcagaaagct caccacgtca aagctctatc cagtttcgga
     3481 acggaactcg atatctttga cactgaactt tctaccggtt gtagaatcct tcacagaaat
     3541 tttccagcgc atgtagacgg ggtttcctat caaatgtcca ttggtgatgg gcgcccgggt
     3601 gtcagaccat ttgtggtgaa gaatgccatc aacatatcgg cgatgagcta tggagccata
     3661 aacaatcgtg cggccgaagc tctgagcctc ggtgctaaag acgtggcgta tgtgaacacg
     3721 ggagaaggtg gatatggccc gcacggagcg gccggaaacg atgtggtgtt tcagattggt
     3781 accgggaaat tcggcgtggg agaagaggct gttcttccgg atggaacagc cactcgcaag
     3841 ctcaacaagg gactccttca agaactagtg cgaaatcatc ccaatatccg gatgattcaa
     3901 atcaagattt cgcagggcgc gaaacctgga attggaggac atctccctgc agacaaagta
     3961 acacctgaaa ttgcggacgt caggcgagtg acgccgtata agactgtgat cagcccgccg
     4021 cagcatgcgg agcttcgagc atcgagtccg aaggaagcag tctccaaact cattgaattc
     4081 atcgaagaga ttcgcaagct cacagagctt ccagtcggaa tcaagctgtg cgtggggaga
     4141 ctcgacgagc tggatttgct tgtactggcc atgaagaaaa ctgggaaagg gcccgatgca
     4201 attcaggtag atggtgcaga tggcggaaca ggtgctgcac ccaatttatt tgtaaattac
     4261 gtaggatacg gaagttccat tgaaacactc cagtatctgg ataagcttct taaaaagcac
     4321 agaatcaggg atcgcgttcg cctgagttcg tccgggcgta tcttcactcc agctcacgca
     4381 gcctatgcgt ttgcaagcgg tgcggataca tgcgaaacgg cgcgcgctgc catgctttcg
     4441 attggttgca tccaggcgca gcagtgtcac acaaacaaat gtcctacagg cattacaacc
     4501 aacagtgcct ggaggatgag gggaattcat attccagaaa aatcaacgcg cgtgcatcat
     4561 ttcttgaaag gctttcacga agacatgctc gagctgacac gcgtcatggg tcattcagat
     4621 ccgcgtgata ttaaactgga tgaccttcgc gtgatgtcgc ggggtggagc gttctcggcg
     4681 ttctttagcg acagtcatgt ttaatcgcgt cattcgcatc gcgggctggc tcgggatcaa
     4741 taccgttctt ggaaccatga acggtctttt gatttctttt tactcaccgg agcctttcgc
     4801 gcgcgttttt ctcacggctc aaatcatgac gcatgtgctc tgcctgctca tagagtttgc
     4861 gatgtttacg gccgggattc agctttatag aatcgggacg cataagaaac aaccggttcg
     4921 ctactttact gcagcgctct gtatcactgc gatctccgct gtgtgcggtg tgcttgctgg
     4981 cgggagaatt caccaggagc tacttgggtt taatccttat gagaacagtc gcttctacaa
     5041 tgtgatcttt ggatccatca tctttgccat tggcctggca agcattgacc gcttctttcg
     5101 ctatctgaga gagaccaggc ttcttgcaga gagatcgttg caggaagcgc gtctggcagt
     5161 actccaggaa agaatgcgcc cgcatttttt gtttaataca ctgaacagca ttcatgcgat
     5221 gcttccagat catgcggacc gagcggatgc agccatcctg atgctcgcgg acagctatcg
     5281 cttcttgctc gggccggcgg aaaagccgct cgtgagtttt atggaagaat gggagttctg
     5341 caaaaactac gtagaactta tgaaggtaag atttggagaa aggttgagtg tttggatgga
     5401 gcacaggggc gatttttcgc gcgtcttgat accaccttta tccatccaac cagtcatcga
     5461 aaatgcattt cgccacggag tgcagaagat acctggaaac gcgatcgtgc aaatttctgc
     5521 tgaatgcgcc gggtcatccg tgcgaattga agtcaaagac aatggaccgg gtcttccctc
     5581 agattttgaa tccaggagca ttgacaatat tcgctctcgg ttacgctttc atttcccggg
     5641 cgcggatctg gaagtttcaa atctgggtga gagtggtgta tcgagcagga taacgctcac
     5701 gggggctgcg taatgccttt gcgatgttat gtgatcgagg acgaaccacc cgcacgtgaa
     5761 attctgtttc gctttattga ggcgagaaaa gatctctctc tggcaggatg ggcgggagat
     5821 gctcgcagcg ctctgaacta tctgaaagtg aaccctgcgg atctagtctt cctggatgtg
     5881 caattgccag gtatgtccgg gtttgaaatg cttgaagagc ttcgccccag tcctgccgtc
     5941 atatttacta ctgccggcca ggcgcacgca gttcccgcat ttgatcacgg ggccgttgat
     6001 tttctattga agcctttttc tatggagcga tttgacattg cagtcaatcg ggtcgtgcag
     6061 aaacggacca cgaatccatc gagcggtgga ctggctgtca cagaaggaga aaatcactat
     6121 ctgattcctt ttggagaaat catttattta tcttcgcgtg gaaaggcaac tgcggttcac
     6181 acaacggatc gcgattttga atcacagaga atcttgaagg acatcgagtc gcgtctgccc
     6241 gaaagctttt tacgaattca taagcagtac atattgaatc tggattttct ggcgcacctg
     6301 aaatacagag ttggcggtgg atatacggcc actctgcgtg attcagacga aacaagcgtt
     6361 ccagttggtc gcggattcgc tggcgccctg cgcgccaggt ggtccggcta gctacattcc
     6421 aagttctttc agacgattgt agaaagtggc gcgcttgaga cccaggattt tggccgcttc
     6481 aaccttgttt cctttcgttt tcgcaagtgc actttcaatg attctccttg cgtgttcatt
     6541 cagaatttct tcgagagggc ggccatccca ctggttgccc ggtgaaggtt tttggatttc
     6601 agccggcgat tccgatctca tttttcgggc atcgagcaat tgcacaggga gatcttctct
     6661 tcgaatgatg tccgtggtgc tattcacaat cgcctggatg actgcgtttt caagttcgcg
     6721 cacgtttcct ggccagtcaa accggcacag agcatccatg gctccatcgc ccagtttcct
     6781 ggcagggtag cccatctcgg cacagtactt ctccgtaaag aattgagcca gagcaggaat
     6841 gtctgctctt cgttcgcgca agggcgggat gcgaatctga aagacattga ttctatagta
     6901 gagatcagat cggaacgttc cctttgcctg catctcttcc aggtttcgat tggttgcgaa
     6961 aatgaatctg caatccgtct taatttcttt tgttccgccc accctttgaa agacgcgctc
     7021 ctgaatgagt cggagcattt tgggctgcat ttcgagcggc atttcgccga tttcatcgaa
     7081 gaagatggtt cccccggcag cctgctcaat ctttccaggt ctgtctgttt ttgcgtccgt
     7141 gaacgcaccc ttcacgtgtc cgaatacttc tgactcccag aggttcccgg gaatggccgc
     7201 gcagttaacc gcgacgaaag gagattctgg catggacgca tcatgaatga gattggccac
     7261 catctctttt cctgttccag tttctccgag aatcagcatg ggctgatttt tccccgcgag
     7321 atttcgcgta cgatccacca gatcgttcat ggacttgctc aaatagacga atgtcttttc
     7381 agacattttg tgttcgcgcg cgttgtgcat atccacaatg tattccgaaa gttgttgcac
     7441 gctttcctct gcttttcctt tcagcagaga gagttcatca tatagcattg cgttgcgaac
     7501 tgcaagagcc gctgaaaaac gaaatgcctc aagaagattc ttgtccgaat accgcatttg
     7561 cgaatcggca gacgcgcccc ctaccatgta tcccagaagt tctgatttga aagtgagtgg
     7621 aatgactgtg cgaaagccat cctgagtcag tcgtttgaga aaggcacgga tcttatttgc
     7681 gttttcggag taggtgcgta tcatgccgtc tggatctttg aaaggaattc cgctctgatc
     7741 ctgaaggtca gcgatggcgg taatttctct gaagaaatct tcaggtggtt tgaatctgcg
     7801 aatgacgtga aacgctctgg gaacaaaatc tgcgacatac gcgggtacct ctccaatccc
     7861 gcgtgaaaca tatctgtcca ttct
//
LOCUS       DAOQJL010000122         7850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5038_length_7850_cov_3.947274, whole genome shotgun sequence.
ACCESSION   DAOQJL010000122 DAOQJL010000000
VERSION     DAOQJL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_7850_cov_3.947274"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="PLG78_07210"
     CDS             <1..1603
                     /locus_tag="PLG78_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE22937.1"
                     /translation="FFISDGNVMRQSAGDTVDFRTRTVPHRNPASRLSLPPGSKSTVY
                     LHVTSKGTIMVPVRIWEADEFLYKAMPEYLIHGLYFGTIASLLVYNFMIFVFVRERSY
                     LYYCLYLSMILVSYLLLNGFAQQFFFQYSPFILNEGLLFVTALAIVSVALFNREFLQN
                     KKVNSNMDKLMMSLIWFAVIVAISSLLLPFMISVRLITALMPLGSIALFLNAIMAARS
                     GVQQSRYFILAWSALAAGSIVESATSFTLLPMSLAGRFGTQLGTVAEVLLFSLALGRR
                     IRRLSEEKGKAQARLSALEKDMELARAIQERILPHSIPVSEAEIKVTYIPLHAVGGDF
                     YDFHETGERSLGILIADVTGHGVSAALDSSTVKVAFRTVCSEIASPGIVLGKMNQFLR
                     TYIQYRFVSAFCIYLDFDAMQMKFAAAGHPPVLVLRQGKVIPLESEGMLLGVIQSFDY
                     KDATFDLQPGDKLILYTDGLYEDLPGEDYTIPLYAQVERCAATTDADAFGETLVHNLS
                     QMRTLLSDDITVITIDIPEPAVALR"
     gene            1603..2382
                     /locus_tag="PLG78_07215"
     CDS             1603..2382
                     /locus_tag="PLG78_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22938.1"
                     /translation="MQTLQYLSFDVAAGAVCAALFAARAARIELDPFVLACLFMAVLC
                     IYNVDHYLDARLRQQKGDARRTYHAEKKNWLLSLAILAFLAAAILMLRLPEIMIAGGL
                     GMLLFLCIYFIIINKFHLRFEKEILVSAGYTAGAWIAALPAFSISLLPYVAMMFCSCW
                     INVLVLSSHDLEVDRLRGEHNAIRAIGADVLGRLIAFIWICGMIAAALSLALDGRWIL
                     TAIAVAHMTLQGVLANQRFGQSARTIGDASYGLYALAWIVA"
     gene            complement(2386..4128)
                     /locus_tag="PLG78_07220"
     CDS             complement(2386..4128)
                     /locus_tag="PLG78_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE22939.1"
                     /translation="MIQGNYFKDNEDLMLQFNSILDWKEIVAAYERGFEDAKTYEKTK
                     DERFANAPADVKSAVEYYESILEATGDLMGTYVAPRSAEMDRVGLKYENGKVTFPKAQ
                     EECYSTLRDAGLMPIGISRKNGGLGLPATVQSMICEIAARADAAFCLAFGNVNIVDIV
                     ERYGSDELIQEWIPDIAAGKYSAAMALTEPNYGSDLPSVQTKAEKGADGVWRLTGTKR
                     FITHACGYIDAPSIILTLARTGSPTSGARGLSFFLVQGKDVHVAGIEKKLGLHCSPTC
                     EVVFDNSPGQLIGEEGFGLVKYSMGMMNAARITIAAQSVGIAEAAYREAKKYASERIQ
                     FGKSIDKIPAVRKMLDRMEREIAGMRMLVLEATRSVDRYVWKKEHLIRHEGKSEKDAK
                     ADPEVKLWEKLADLFTPMAKYYASEGCVKIAYDALQIFGGAGYTEEYDVARIYRDARI
                     TTIYEGTTQLQVVAAIGGIVSGMAPAGNLREYIQQEMKSISPSAELKRSFDLFSEIAE
                     AYRDLPAGEEKSMLAFETVEAATRLLMGLLMERSLARTPDNAHRKEMCRGVSIDTIAT
                     LEGTRARMKLSAVK"
     gene            4220..4828
                     /locus_tag="PLG78_07225"
     CDS             4220..4828
                     /locus_tag="PLG78_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22940.1"
                     /translation="MRKLIATFLTTTLSAAALVADPQLARTTAGKYVRLDDDGKWTYL
                     TESGLNEFLGKTQAGELKSTIFPGAGLKFQSGKWNLTNSTNKDAEFTLRDLGDGLYCM
                     FIPEKGSFSLELLKQAVVENAKSAVDSYNIVSEERRVINGKEFLILKAAITMKRTNFL
                     FKYALYSQDGYSLQTIVYGLEEPFKANEAIADKLLFEGVIVP"
     gene            complement(4830..6194)
                     /locus_tag="PLG78_07230"
     CDS             complement(4830..6194)
                     /locus_tag="PLG78_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNE22941.1"
                     /translation="MNRKTATSALVIFFCAIGNLNAWSEHSIFMHSALSGMKEIAGAA
                     PVAAETLDSFLAKESKGLAQLLSEEESWARANIEGYPARPDDLAFDRSTETNLRKRFL
                     YALRVNPEAKMAMALQLMPGQDPAGRTFLTPRDVSVYKNPIWLAKIRFAQIRPGERVA
                     PLEILSTAADEPDLGLDIGLFEDSGTEWGSKMGFGKIPFGNANLEYSSQAPFHMTFYH
                     EAGIVYMLAGFLKHTYPEVRVRQFYALSRYAFRTGHPYWGYRFMGMGLHYVTDLTQPY
                     HARVLPGIGVLRMLWINLMDIAGRHQPKLDAIQLVSNRHSAIENFYQVILHRDSVAHD
                     MNHPYWKATVDESKDLSYGKFGRKYLKDVVTQESESRADALDSKLTDLIPSNLVSDPS
                     VEFAVANAGKDLTKVMEKEKPGSVDQLSLVLADFAASFGSHSRNFIRAVLALEDAYAS
                     SNAK"
     gene            complement(6239..6718)
                     /locus_tag="PLG78_07235"
     CDS             complement(6239..6718)
                     /locus_tag="PLG78_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22942.1"
                     /translation="MLNLLKWPFLLLWRLLTLGAGRGRIGEVADRIARHGDRFANARQ
                     NEKASIARQYSGRARNAFTVGGAESLEEDFLVAVGLKAARQVREAVESDANDLTRDSW
                     LRNSSSNSRSSESASSGFVSSSSSSDSSSSESSVEAGGGSFGGGGASGGWDNPSDNS"
     gene            complement(6712..7626)
                     /locus_tag="PLG78_07240"
     CDS             complement(6712..7626)
                     /locus_tag="PLG78_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22943.1"
                     /translation="MSLIVITSYYNPEHYRSRFENFGRFVAGLESAGIRWMVGELAFG
                     EDDFELPDHPSIVRMRGGDVMWQKERILNLLLDQLPSDAKYVAWMDCDLLFENPDWAR
                     ETERNLKSDAVVQLFDYVVRLPRGHASYRGEGEGWEGIGSIQRRSPNVLLGGDFAAHG
                     HTGFAWAAHRSILEKVRLYDACIAGSGDHMMAHAFYGDWHSACVDRILCRNSFHVSHF
                     ERWCRAVYPLVRAKVGFTRGAIYHLWHGETTDRRYVLRNRELAAFGFDPVRDIRVGPE
                     GLWEWTGKKSGLPDWARSYFKQRKEDAC"
     gene            complement(7634..>7850)
                     /locus_tag="PLG78_07245"
     CDS             complement(7634..>7850)
                     /locus_tag="PLG78_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22944.1"
                     /translation="VNPEAKRLLANPEACEKMFAGLERSSQTMAFIPIIEDCARKTAC
                     SELETFKCFALSATRSIPTKSDSSVSP"
BASE COUNT         1866 a         2124 c         1871 g         1989 t
ORIGIN      
        1 gttttttatc tcggacggga acgttatgcg ccaatcagcc ggagatacgg ttgattttcg
       61 cacgcgcacc gtgccacatc gcaatccagc gtcccgtctc tcattgcctc cgggatccaa
      121 aagcacggtc tacctgcacg tgacgtccaa aggaaccatc atggtcccgg ttcggatctg
      181 ggaagcggac gagtttttgt acaaggcaat gcctgaatac ttgattcatg gcctgtattt
      241 tggaacgatc gcgtctttac ttgtctataa cttcatgatc tttgttttcg tgcgtgaaag
      301 aagttatctg tactattgcc tgtatctgtc gatgattctg gtttcctatt tgctcctcaa
      361 tggcttcgca cagcagtttt tcttccagta ttctcctttc attctcaacg aaggtttact
      421 attcgtgacc gctcttgcga tcgtgagtgt ggcgctgttc aaccgggaat tcctgcaaaa
      481 caagaaagta aattcaaaca tggacaaact catgatgtcc ctgatctggt ttgcagtcat
      541 tgtagccata tcctccttgc tccttccctt catgatctct gttcgtctca tcactgcgct
      601 gatgcccctg ggttcgatcg cattgtttct aaatgcaatc atggcggcaa gaagcggagt
      661 gcaacaatct cgctacttta tcctggcctg gagcgcactg gcggcaggat ccatagttga
      721 gtctgctacc tctttcactc tgctgcccat gtctcttgca ggccgatttg gaacgcagct
      781 gggcacagtg gcagaagtgc ttcttttttc tctcgctctc ggccgcagaa tcaggcgcct
      841 ttcagaagaa aaaggaaaag cacaggccag actatccgca ctggaaaaag atatggagct
      901 ggcgcgagcg attcaagagc gtatcctccc ccattcgatt cccgtaagcg aagcagaaat
      961 aaaagtaaca tatattcccc tgcatgcggt gggtggagat ttttacgact tccatgaaac
     1021 aggcgaaaga tctctcggca ttttgatcgc agacgtgacg ggccacggtg tttctgctgc
     1081 actcgactca tccacagtca aagtggcatt tcgcacagtt tgttcagaga tagcatctcc
     1141 gggaattgtc cttggtaaga tgaatcaatt ccttcgcaca tacatccagt acagatttgt
     1201 gagcgcattt tgcatctacc ttgatttcga tgcgatgcaa atgaagttcg ccgctgcagg
     1261 acatccccct gtccttgtac tgcggcaagg taaagtaatc cctctcgaat cagaaggaat
     1321 gctgctgggc gtaatacaat cgttcgatta caaggatgcc accttcgacc ttcagcccgg
     1381 ggacaaactg attctctata cggacggatt gtacgaggat cttccaggtg aagactacac
     1441 gattccgctc tatgcccagg tggaaaggtg tgctgccacc acagatgcag atgcgttcgg
     1501 agaaacactc gttcacaatc taagccagat gcgaactctt ctgtcagatg atatcacggt
     1561 gattacgatc gatattccag aaccagcggt agctctgcgc tgatgcaaac actgcagtac
     1621 ctgagcttcg acgtcgcggc gggtgcggtc tgcgcggctc tgtttgcagc gcgggccgcc
     1681 agaatagagc tggatccttt tgttctggcg tgtctattca tggccgtact gtgcatctat
     1741 aacgtggacc actatctgga cgcaagactc agacagcaga aaggagacgc acgcagaacc
     1801 tatcatgcag aaaaaaagaa ctggctcttg tccctcgcca ttctggcctt tctcgcggcc
     1861 gcgattttga tgcttcgcct gcctgagatc atgatcgcag gtggactcgg tatgttgctg
     1921 ttcctgtgca tttacttcat catcataaac aaattccatc tcagatttga aaaagaaatt
     1981 cttgtttctg caggctacac tgcaggtgcg tggatcgcgg ctctgcccgc cttttctata
     2041 tcgctcttgc cgtatgtagc gatgatgttc tgctcctgct ggatcaatgt attggtacta
     2101 tccagccatg acctggaagt ggacaggctt cgcggagagc acaatgcaat tcgtgccatt
     2161 ggagcggatg tacttggccg cttgattgca ttcatctgga tttgcggaat gatcgcggca
     2221 gcactttcgc ttgccctgga cggtagatgg atcctcaccg caatcgcagt ggcacacatg
     2281 actttgcagg gagtgcttgc gaatcaacgg ttcgggcaga gcgcccgaac cattggggat
     2341 gcgagttacg ggctctatgc ccttgcctgg atcgtcgcct gacgtttact tcactgcaga
     2401 aagcttcatg cgcgcgcgtg ttccttccag agtggcaatc gtatcgatgc tcacaccgcg
     2461 acacatttct ttgcgatgag cgttatcagg agtacgcgcc agagagcgct ccatgagaag
     2521 gcccatcagg agacgtgtgg ctgcttccac tgtttcaaac gcaagcatac ttttctcttc
     2581 cccggctggt agatctctgt atgcctctgc aatctcagag aacaaatcaa aggatctctt
     2641 gagttccgct gacggagaaa tcgacttcat ttcttgctga atgtattcgc gcagatttcc
     2701 tgctggggcc atgccagaaa caatgccacc gatggctgcc actacctgga gttgcgttgt
     2761 gccttcgtaa atggttgtaa tgcgtgcatc gcgataaatg cgtgccacat catactcttc
     2821 cgtatagccc gcgcctccga aaatctgtag cgcgtcgtat gcaatcttca cacagccttc
     2881 gcttgcgtaa tattttgcca tgggtgtaaa caaatccgcg agcttttccc agagtttcac
     2941 ttccggatct gctttggcgt ctttttcact cttcccttcg tgtctgatca gatgctcttt
     3001 cttccataca tagcgatcca cagatcgagt tgcttcgaga accagcattc gcattccagc
     3061 gatttcgcgc tccatgcggt cgagcatctt gcgcactgca ggaatcttat caatggattt
     3121 tccaaattga attctttctg atgcgtactt cttagcttca cggtaggctg cctctgcaat
     3181 gcccacagat tgtgccgcaa tggtaatgcg cgcagcattc atcatgccca tagagtactt
     3241 cacaagacca aagccctctt ctccgatcag ttggccagga gagttgtcaa acaccacttc
     3301 acacgttggc gaacagtgaa gcccgagctt tttttcaatt cccgccacat gcacgtcttt
     3361 gccctgaacc aggaaaaagg aaagtccacg agctccgctc gttggagatc cagtgcgagc
     3421 cagagtgaga atgatgcttg gagcatcaat atagccgcat gcatgagtga tgaatcgttt
     3481 ggttccagtc aggcgccaga cgccgtctgc gcccttctct gctttggttt gaacactggg
     3541 gagatccgat ccgtagtttg gttccgtcaa tgccatcgca gcgctgtact ttcctgccgc
     3601 aatgtccgga atccattctt gaatcaactc gtctgagcca tatctctcta caatatctac
     3661 aatgttcacg tttccaaacg caagacagaa ggctgcgtcc gctctcgcag caatttcgca
     3721 gatcatggat tgaacagttg ccggcagacc caggcctccg ttcttacgac tgattccaat
     3781 cggcatgagg ccagcatcac gcagagtgga ataacactct tcctgcgctt ttgggaaagt
     3841 tacttttccg ttctcatact tcaggcccac ccgatccatc tcagcacttc gcggtgcgac
     3901 gtaagttccc attaaatcac cggtggcttc caggatagat tcgtaatact ctacagcaga
     3961 tttcacatct gccggagcat tcgcgaacct ttcgtccttt gtcttttcat aagtttttgc
     4021 gtcttcaaat ccgcgttcat aggccgcgac gatttccttc cagtctagaa tagaattgaa
     4081 ctggagcatc aagtcttcgt tatctttaaa atagtttccc tggatcattg ccttcctcca
     4141 aagtcaaact gggactcaat ggggcgttgc atataaaaaa aggtctagac ctgaccgacg
     4201 gcgttctgtc tgatcagcca tgcgcaaact gatcgcaacc tttttgacca ccaccctgtc
     4261 ggcggctgca cttgtggctg atccccagct tgcacgaacc accgcgggca aatacgttcg
     4321 cctggacgac gacggaaaat ggacctactt aacagaatca ggcctgaacg aattcctggg
     4381 aaagacacag gcgggagaat tgaagagtac catctttcct ggagctggcc tcaaatttca
     4441 atcgggcaag tggaacctga caaacagcac gaacaaagac gctgagttca cattgcgcga
     4501 tctgggtgac ggtttgtatt gcatgttcat accagaaaag ggaagcttca gtctggaatt
     4561 gctcaagcaa gctgtggttg aaaatgcgaa gagcgcagtg gactcttaca acatcgtttc
     4621 agaagaaagg cgtgtgatca acggaaaaga attccttatt ctaaaggctg ccatcaccat
     4681 gaagcgcacc aattttctct tcaagtatgc gctctattct caggatggat actcactcca
     4741 gaccattgta tacggtctgg aggagccgtt caaagcaaac gaggcaatcg cagacaaact
     4801 tctgtttgaa ggcgtgatcg ttccctgacc tacttcgcat tcgaggacgc gtaggcgtcc
     4861 tcaagtgcaa ggactgctct gatgaagttt cgcgaatgcg atccaaagga cgccgcgaaa
     4921 tcagcaagaa ccagggacaa ttgatccacg gaccctggct tttctttctc catcactttc
     4981 gtaagatcct ttccagcatt cgcaaccgca aactcaacag atggatcgct cacgaggttc
     5041 gatggaatta gatccgtgag ctttgagtcc agcgcgtctg cgcgagattc agattcctga
     5101 gtgacaacat ctttcaaata tttccggcca aatttcccgt acgacaggtc tttgctttca
     5161 tctacagttg ctttccagta tggatggttc atgtcgtgcg cgactgagtc tctgtgaaga
     5221 atcacttgat agaaattttc aatcgcacta tggcgattgg agacgagctg gatggcgtct
     5281 aactttggtt gatggcgtcc agcaatgtcc atcaagttga tccagagcat tctgagcaca
     5341 ccgattccgg gaaggacccg cgcatggtaa ggttgagtta ggtccgtcac atagtgaaga
     5401 cccattccca taaaacgata gccccagtac ggatgacccg tgcgaaatgc ataccgcgat
     5461 agcgcgtaga actgacgcac tcgaacttca ggatacgtgt gctttaagaa tcctgcgagc
     5521 atatacacga ttcccgcctc atgatagaac gtcatgtgaa atggagcttg agaactgtat
     5581 tcgaggttgg cgtttccaaa tggaatctta ccaaaaccca tcttgctccc ccattcagta
     5641 ccgctgtctt caaatagccc gatgtccaga cccaggtctg gctcgtctgc ggcggtggac
     5701 aaaatctcga ggggtgcgac gcgttctcct ggacgaatct gcgcgaaacg aatcttcgca
     5761 agccagattg gatttttgta aacagataca tctcgcggag tcaaaaaagt tctgcccgcg
     5821 ggatcctgac ctggcatgag ctgcagtgcc atggccatct tcgcttctgg gtttactctg
     5881 agagcgtaca ggaagcgctt gcgcaagttc gtctctgtag agcgatcgaa agcaaggtca
     5941 tctggtctgg caggatatcc ctcgatgttc gcacgcgccc aggattcttc ttcagacaga
     6001 agctgcgcga gtcccttgct ttcctttgcc agaaatgaat ccagagtctc cgcggccact
     6061 ggcgcggctc cggcaatttc tttcattcca ctgagggctg aatgcatgaa gatagaatgc
     6121 tcactccacg cgttcagatt tccaatggca cagaagaaga ttacaagagc agaagtcgcc
     6181 gtttttcgat tcataagcaa tcctatagaa atattcgtcc gaggtccagg ctaaaatgtc
     6241 agctgttgtc cgagggattg tcccagccgc cgctcgcccc gccgccgcca aaactccctc
     6301 cccctgcttc aacggatgat tccgaagaag aagaatctga actggaagag gaggatacaa
     6361 atccgctgga cgcggattcg ctactcctcg agttgcttga ggaattgcga agccaggagt
     6421 cgcgcgtcag atcatttgca tctgattcta cggcttctct gacctggcga gccgctttca
     6481 agcctacggc cacgagaaaa tcttcttcca gggattctgc gccgcccaca gtgaatgcat
     6541 ttcgcgcgcg cccagaatac tgccttgcga tggatgcctt ttcattctga cgggcgtttg
     6601 caaaacgatc gccatgacgg gcaattcgat ctgccacctc tccaatccgc ccgcgccccg
     6661 ctcccagagt caggaggcgc cacaacaaaa gaaatggcca tttcagaaga ttcaacatgc
     6721 gtcctctttc cgttgcttaa agtaagaccg ggcccaatca ggcaaaccgc ttttttttcc
     6781 agtccactcc cacagaccct ccgggccgac acgaatgtca cgcacaggat caaaaccaaa
     6841 ggccgccagc tctctattgc gaagcacata ccttcgatcc gtggtttctc cgtgccatag
     6901 atgatagatc gcacctctgg tgaatcctac cttcgcgcga accagaggat acacagcgcg
     6961 acaccatctt tcaaagtgag atacatgaaa tgaatttctg caaaggattc gatccacaca
     7021 tgcagaatgc cagtctccgt agaaagcatg agccatcatg tgatctccgc tgcctgcaat
     7081 gcaggcatcg tataaacgta ccttctcaag aatcgagcgg tgagcggccc aggcaaaacc
     7141 tgtgtgtccg tgcgctgcaa aatcccctcc caaaagaacg ttaggcgatc ttctctgaat
     7201 ggacccgatt ccctcccatc cctctccttc tccccgataa gacgcgtgcc ctctggggag
     7261 acgaaccacg tagtcaaaca actgaactac ggcatccgac ttcagattcc gttcggtctc
     7321 gcgtgcccag tccggattct caaaaagcaa atcgcaatcc atccaggcca cgtactttgc
     7381 gtcggagggc aattgatcca atagcaaatt caggattctc tccttctgcc acatcacatc
     7441 ccctccgcgc attctcacga tcgaggggtg atcagggagc tcaaagtcgt cttctccaaa
     7501 agcaagttcc cccaccatcc aacgaatgcc ggcagactca agccccgcta caaagcgccc
     7561 aaagttttcg aatcgcgaac gatagtgttc tgggttgtag tacgatgtga taacaatcag
     7621 agacatgcgg cgatcaaggt gaaacggaag agtctgattt tgtcgggatg gatcgggtcg
     7681 cactcaatgc gaagcatttg aacgtttcta gctccgagca tgcagtcttt ctcgcgcagt
     7741 cctcgatgat tggaatgaat gccattgtct gagaacttct ctccagaccc gcgaacatct
     7801 tctcgcacgc ctcgggattg gccaggagtc tttttgcctc agggttcacc
//
LOCUS       DAOQJL010000123         7845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5042_length_7845_cov_3.993967, whole genome shotgun sequence.
ACCESSION   DAOQJL010000123 DAOQJL010000000
VERSION     DAOQJL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_7845_cov_3.993967"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLG78_07250"
     CDS             complement(<1..356)
                     /locus_tag="PLG78_07250"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNE22945.1"
                     /translation="MSCPYQPLLSTYGPPNVKWCEESLCAIVQEPANTWSNLLFIFVG
                     MWILTQARKRRPFVALGLAITIMGTLSLVYHASNLYPTQLGDFLGMFLYLSLLHMWNI
                     RRAGVALTQNASVIVY"
     gene            441..1334
                     /locus_tag="PLG78_07255"
     CDS             441..1334
                     /locus_tag="PLG78_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE22946.1"
                     /translation="MIFRYVAFFLLAVWIFAPLFAQPVEDRTTLKSERPVSFVSAISG
                     LFLRQSPGTDAPTIRLLKYATPLEVLKRKEPFERVGDKEGQWLLVKTEDVTGWVFGAY
                     VTHAIPASPIRHWNASHTMYFVVTSKAPGGDCASAYNYDQPCWISAFDSRGNVMRPYQ
                     GAHSGSVGSAGWEKDEIELFTYGVDGGGGGSSNTLWSPVTGQEHEIFHSYFIPNRNKD
                     GETTSVLFEICFKDKCHFIEQFEKTHTCDVWQGQTEKRVRKLQTFRYKRKCDLTLADH
                     RPQLNVDDAVYFIVDSELQRQ"
     gene            1418..1780
                     /locus_tag="PLG78_07260"
     CDS             1418..1780
                     /locus_tag="PLG78_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22947.1"
                     /translation="MKFVKAVLMIALVAMASLSLVQCAPKSPSVAKVEKSLDDLKAGI
                     EANGTDCAKIAAAIKTPASDLTAALKEVKEKNEKLPATTQVKFAGLVSSFQKIGACSK
                     TPEMQAVFESLVASATAQ"
     gene            complement(1788..3062)
                     /locus_tag="PLG78_07265"
     CDS             complement(1788..3062)
                     /locus_tag="PLG78_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE22948.1"
                     /translation="MAKLERFWDWLTDRARVGHLSRDEIRAFAILNMICLMAMVSIVT
                     VTTLILVAAGPLRQTRFDVVQNAISLVLYGLCWILVRRGNAVISSVILSLFGNLQITT
                     LVLYYGFGGGIQWYYGVLMAAPLVTIPGKRQPLRMVLASLPLILFLWTHYEFRIRGRM
                     PLAEADPGVVIFFFSNSLSSLLILGVMVAYFRYAAVRAESELEVERRRSDALLLNILP
                     ESIAGRLKLGEKTIADRFENVTVLFADLVHFTKMSAEIPPEEIVRILDEIFSSFDEAA
                     ARLRLEKVKTIGDAYMAIAGAPEEKPDHAHRAVLFGLEMLSILGIISDRLGRKLDLRI
                     GIHTGPVVGGVIGRDKIAYDFWGDTVNTASRMESHGEAGSIHISEETRREIEGGFVTR
                     ERGRMEIKGKGVLQTYFVEARQPAGDGTTAGS"
     gene            complement(3049..3585)
                     /locus_tag="PLG78_07270"
     CDS             complement(3049..3585)
                     /locus_tag="PLG78_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22949.1"
                     /translation="MKFPGNLTKCFDKDLYLMFLDGFEFVPDFDSEDFTAWFRTPATD
                     HRNYLCRFAQEGTGGLFCFWKKDGGDDYSNSPVIYLGSEGERGVAARNFDDFLRVLSS
                     GFILFSLVFAGKQYHFSEAEEDPEEKKENQATFDEFVSWLRAECSIEPAKDVLRMQSD
                     LADLNAEFQSWIEARGKT"
     gene            complement(3590..4204)
                     /locus_tag="PLG78_07275"
     CDS             complement(3590..4204)
                     /locus_tag="PLG78_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE22950.1"
                     /translation="MLAGCARNFPVAIRDEEIAAFSMLAANLSRAAVPAVICQNRGFC
                     YIFWSASTYNGQPSPVGPAGMDARCNADGNKPNTSQYKALLSGGTRRATTTANLGDGQ
                     LDWVLYASMTYKRPDGTLIGSTNAAKLLIFPLTNGISPSAGSGTWTGMNIDWTDNTDI
                     CTGWTSTGGFGTIGTANATTTGALYQTNAQGCGAVIFNIYCVEQ"
     gene            complement(4417..5541)
                     /locus_tag="PLG78_07280"
     CDS             complement(4417..5541)
                     /locus_tag="PLG78_07280"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNE22951.1"
                     /translation="MQSVSCPKCSHKFELSKALESEMEAKLRESLEEEQTRWKQEEAS
                     KLEKRLRAEAKDRLSVEMQALQESVAEKEKALREARSSELEVRRKMRSLEEKSAAFEI
                     EKQNILDDERKKIALQAELAAEEKYKLKIVEMQTERTSLASRIDELQKKAEKTSQELQ
                     GTAQEIGLMDLLQKHFPGDRFERVGKGKKGGDVIHSVVVNGKVCARIVYESKRTAAWS
                     NTWIEKLRTDRNNEKADLAIIVSQALPDSIRSAGCMEGIWICDFQTAPWLSAALRNGL
                     IELARVRSAMESQSDMKDIVWRYLTGNEFKNRIERMLRTFMSMKQQIERERAQALKWL
                     SERDQQLNLIAEDVNGFRGELEAMLQVSLTGVQSLQLAAG"
     gene            complement(5626..6012)
                     /locus_tag="PLG78_07285"
     CDS             complement(5626..6012)
                     /locus_tag="PLG78_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22952.1"
                     /translation="MKKLHLAVGIALLLVFVSTGAFMKWEQLHTDGLDPLYRMMHRSR
                     HIYILFAGLIHLMAYRAYDDGLSGTRSILLRISSGFLFASAVLFPIAFLVEPQLHRVA
                     APVTFFGVLLTLIGVLLPVVSGFMRK"
     gene            complement(6089..6997)
                     /locus_tag="PLG78_07290"
     CDS             complement(6089..6997)
                     /locus_tag="PLG78_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE22953.1"
                     /translation="METFLRIVLGILFLPLVSCQQTPAGMAKLPSGTYFRGLQISRKA
                     DQRPRHKVRIRSFYLDETLVTVADFRRFVERTGYQTSAERMGYAMISLEGMADWKWKQ
                     ARGASWKNPFGELRNVPVHEEDPVTAVSWEDANRYCAMVGKRLPTEAEWEYAARAGSD
                     LRFPWGDSPMRKGAFGLNFWQTEHHEHGSLEDGYLYLSPVRAYPPNDWGMYDPVGNVW
                     QLTADYYSPYAYEDAASEADQNPDRAVANPKGPDHGYERVARGGSWWCSENTCNGFGL
                     YYRGHTAPVAPFNNQGFRCAKDLPVD"
     gene            complement(7013..7618)
                     /locus_tag="PLG78_07295"
     CDS             complement(7013..7618)
                     /locus_tag="PLG78_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE22954.1"
                     /translation="MTAPSGLYVRKEADAKSKPVALLKKGAYFHFSGVAGAPVTIDSI
                     SSRWLKYSGPAGTGYVFGGFVSREPPLLVYTREQINEIEKENRAYVESLAREELRRME
                     EAQKAGQFYQNCDACEHLKIDWRRVELRAGCDQNARERIHQLVIERIPGYDKRGINDE
                     ATRQAFLDAAEKVDNPYCEQLLMNSWQSQGPAQCNGPSFFE"
BASE COUNT         1977 a         2100 c         1854 g         1914 t
ORIGIN      
        1 aaatagacga tcaccgaagc attctgggtc aacgcaactc ccgcgcggcg gatgttccac
       61 atatgaagca agctcagata gagaaacatt cccagaaaat caccgagctg cgtaggataa
      121 agattcgagg cgtgatacac cagagaaagc gttcccatga tggtgatagc cagaccaaga
      181 gccacaaaag gcctcctctt acgagcttga gtgagaatcc acatgccaac aaaaatgaac
      241 aaaaggttgc tccatgtatt ggcaggctcc tgcacaatcg cgcacaggct ctcttcgcac
      301 catttcacat tgggagggcc gtatgtactg agtaaaggtt gatagggaca gctcatggct
      361 caacacgcaa tgcggaacaa aaacgtccag aacgaatttc gtttgaccgg cggcaaaata
      421 cgcattgtca tcattctgca atgatctttc gctatgtggc tttcttcctg cttgcagtat
      481 ggatcttcgc gcctctcttt gcacaaccag tagaggatcg aaccaccctg aaatcagaac
      541 gtcctgtcag cttcgtaagc gcaatttccg ggctatttct aaggcaatcc cctggaacgg
      601 atgctcccac cattcgtctt ttgaaatatg caactccact ggaagttctc aaacgaaagg
      661 aaccatttga gcgagtagga gacaaagagg gccagtggct tctggtcaag accgaagatg
      721 tcacgggatg ggtctttggt gcctacgtca ctcacgcgat tcctgcttct cccattcgac
      781 actggaatgc atctcatact atgtacttcg tggtaacgtc aaaagcaccc ggaggagact
      841 gcgcgtccgc atacaactac gatcagccgt gttggatttc agcctttgat tccagaggca
      901 acgttatgcg accttaccag ggagctcact caggttcggt tggatctgca ggatgggaga
      961 aagacgaaat tgaactcttc acttatggag tcgatggcgg aggtggaggg tcctcaaaca
     1021 cactgtggtc tcctgtgaca ggccaggaac acgagatctt tcacagctac tttattccaa
     1081 acagaaacaa agatggcgag acaacttccg ttctattcga aatctgcttc aaagacaaat
     1141 gtcatttcat agagcaattt gaaaaaactc acacatgcga cgtctggcag ggacaaacag
     1201 aaaagagagt gcgaaagctt cagacgtttc gttacaaaag aaagtgtgac ctgacccttg
     1261 cagatcatcg gccccaactc aacgtagacg acgcagtgta tttcattgta gactcggaac
     1321 tgcagcggca atagcccaaa aaaccggggc gcaaagaggc gcaattcttc attgacacgc
     1381 gactactccg gaagcgatcc atctacagga gtacaaaatg aaatttgtga aagcagtatt
     1441 gatgatcgcg cttgttgcca tggcatcgct gagcctggtt cagtgcgccc caaaaagtcc
     1501 ttccgttgcg aaagtggaaa aaagcctgga tgacctcaag gcaggaatcg aagcgaatgg
     1561 cacagactgt gcaaagattg ccgcagccat caagacacct gcgagcgatc tgacagccgc
     1621 tctcaaggaa gtaaaagaga aaaatgaaaa gctcccggcc acaactcagg taaaattcgc
     1681 cggactcgta tcttcttttc agaagattgg cgcttgctcc aagactccag aaatgcaggc
     1741 cgtgtttgaa agcctcgtag cgtccgcaac ggcgcagtga tagcgtgtca actgccggcg
     1801 gttgtgccgt cgccggcggg ttgcctggcc tccacaaaat aagtctgtag tacgcccttc
     1861 cctttgatct ccattctacc gcgctcgcgc gttacaaatc caccttcaat ctcacgacga
     1921 gtctcctcgc tgatatgaat gcttcccgcc tctccgtgcg attccatcct gctcgcagta
     1981 ttgactgtat ctccccagaa atcataggcg atcttatcgc ggccaatcac accgcccaca
     2041 accggtccgg tatgaattcc aattctcaaa tccagtttcc ttcccagccg atcgctgatg
     2101 attccgagaa tggaaagcat ctctagcccg aagagtaccg cgcgatgcgc atgatctggt
     2161 ttctcttcgg gcgcccctgc aatcgccatg tatgcgtccc cgatggtttt tactttttcg
     2221 agtctaagtc gagcggccgc ttcatcaaac gaggaaaaga tctcgtcgag aatccggacg
     2281 atttcctctg gcggaatttc cgccgacatt ttcgtaaaat gcactagatc cgcaaacagt
     2341 acggtcacat tctcgaaccg atccgcaatt gttttttcgc cgagcttcag tctcccggca
     2401 atcgattctg gaagaatatt caaaagcaac gcatctgaac gtctgcgctc cacctccagt
     2461 tcagattctg ccctcactgc cgcataacgg aagtatgcca ccataacgcc cagaatcaag
     2521 agagaagaaa gcgagttaga aaaaaagaag atcacaacac ctggatctgc ctcggccagg
     2581 ggcatcctgc cgcggattct aaattcataa tgcgtccaga gaaacaaaat caaaggaaga
     2641 gaagcaagaa ccattctgag aggttgtctt tttcctggga tggtgaccag cggcgcggcc
     2701 atgagcactc catagtacca ttgaattcct ccgccgaatc catagtacag taccagggtg
     2761 gtgatctgga gatttccaaa tagagaaaga atcacgctcg aaatgactgc atttcccctt
     2821 cgcactagaa tccagcacag gccatacagt acaagggaga ttgcgttctg gactacgtca
     2881 aaccgagtct gacgcaaagg acccgctgcc acgaggatca aagtggtcac ggtgacgatg
     2941 gaaaccatgg ccatcagaca aatcatattt agaatcgcga atgcgcgaat ttcatcgcgc
     3001 gaaagatgcc ccacacgcgc gcgatcggtc agccagtccc agaatcgttc aagttttgcc
     3061 acgtgcttct atccaggact gaaactcagc attcaaatct gcaagatcgg attgcattcg
     3121 caaaacatcc tttgccggtt cgatggaaca ctcagcgcga agccatgaaa cgaactcatc
     3181 aaaggttgcc tggttttctt ttttctcttc cgggtcctct tctgcttcgc tgaagtgata
     3241 ttgtttaccc gcaaacacca gggaaaatag aatgaaacct gaagatagaa cgcgtaagaa
     3301 atcgtcgaag ttccgtgcag ccacgcccct ttccccttct gagcccaggt agattacagg
     3361 agaatttgaa taatcatctc ctccgtcctt tttccagaaa cagaacaagc cgcccgttcc
     3421 ttcctgtgca aacctgcaga gatagtttct gtgatcggta gcgggcgtgc gaaaccacgc
     3481 ggtgaagtcc tcactgtcaa agtccggcac gaattcaaag ccgtccagga acatcagata
     3541 cagatcctta tcaaagcact tcgtgaggtt gcctggaaat ttcaaaacac tattgctcca
     3601 cacaataaat attgaaaatg accgcaccgc agccctgcgc gttggtctgg taaagagcgc
     3661 ccgtggttgt tgcgttggcc gtgccgattg tgccaaagcc gccggtggat gtccagcctg
     3721 tgcagatatc tgtattgtcc gtccagtcta tgttcattcc ggtccaggtt ccagagccgg
     3781 cggatggcga aattccattg gtgagaggaa agataagcaa ttttgcagca ttggtagatc
     3841 cgatcagagt accatcgggc cgtttgtaag tcatgctcgc gtaaagaacc cagtctagct
     3901 gaccatctcc cagattcgca gttgtggtgg ctcgccttgt gccaccggaa agcagagctt
     3961 tgtactgact tgtgttgggc ttatttccat ccgcattgca cctggcatcc atacctgcag
     4021 gcccgacagg gctgggttgt ccattgtagg tgctcgcgga ccagaatata taacaaaagc
     4081 cgcgattctg acagatcaca gctggaacag cagcgcgaga caaattggcc gccagcatag
     4141 aaaaagcggc gatttcctca tcacggattg ccaccgggaa attgcgagca cagccggcta
     4201 acatacaccc gcacagacaa acaccgattc gcattgcatg gaacagacgt ttgaactgtt
     4261 tcaggatggt aagatttttg ttcctcaaga aaagtcacgc taccggctca caatttcgcc
     4321 cggttttcgc cttgcgtgca accttttttt ctttcgtcag gtgttgtcct ccttcctgca
     4381 ttcatcgcag tctctgaaat aagcggacgc gagaacttaa cccgccgcca gttgcagaga
     4441 ctgaacacca gtcaacgaga cttgaagcat ggcctcgagc tctccgcgaa atccgttcac
     4501 gtcttctgcg atcaaattga gttgctggtc gcgttctgaa agccatttca atgcctgtgc
     4561 gcgctcccgt tcaatctgct gcttcataga catgaatgtc cgaagcattc gctcgattct
     4621 atttttgaac tcgttgccgg taaggtatcg ccagacaatg tctttcatat cagattgact
     4681 ttccattgca gaacgcacgc gagcaagctc aatgaggccg tttcgcagag cggcactgag
     4741 ccagggagca gtctgaaaat cgcagatcca gattccttcc atgcaaccgg ctgatcgaat
     4801 ggagtcaggc agtgcctgag atacaatgat agcaagatcc gccttttcgt tgtttctgtc
     4861 cgtgcgcagt ttctcaatcc aggtattcga ccaggctgca gttcgtttgc tttcataaac
     4921 gattctagcg cagacttttc catttacgac gacagagtga atgacatccc ctcctttttt
     4981 tcctttgccc actcgttcaa agcggtcccc cggaaaatgc ttctgcaaga ggtccatcag
     5041 tccaatctcc tgagcggtgc cctgcaattc ctgactggtc ttctccgctt ttttctgtag
     5101 ctcgtctatt ctagatgcaa gagaagtgcg ctccgtttgc atttccacga tcttgagttt
     5161 gtatttttcc tctgctgcca gttccgcctg caaggcgatc ttcttgcgtt cgtcatcgag
     5221 gatattctgc ttttcaattt caaaggcggc ggacttttct tcgagggaac gcatctttcg
     5281 tcgcacttcc agttctgaag atcgagcttc tctcagcgct ttttcttttt ctgcgacgga
     5341 ctcctgcaat gcttgcattt caacgctgag ccgatccttt gcttccgcac gcagtctctt
     5401 ttcgagcttg ctggcttcct cctgtttcca tctggtctgc tcttcctcca ggctttcgcg
     5461 aagctttgcc tccatttcac tttcgagcgc tttgctcagt tcgaacttat gggaacattt
     5521 gggacagctg acagattgca tcttcacggg ggcctccaga tgactggatt gtatccgggg
     5581 ggtgtgacat gtttggatac ggaaatgtag atcttacccg gggcgctatt ttcgcataaa
     5641 tcctgaaacg acagggagaa gaacaccgat caaagtaagc aacactccga agaaagtcac
     5701 tggagccgcg actcggtgga gctggggctc tactagaaac gcaatgggaa aaaggacggc
     5761 agatgcaaag agaaatcccg aggaaatcct tagcaagatc gatcttgtgc cacttaagcc
     5821 gtcgtcgtaa gcacgatacg ccatcaaatg aatcagccct gcaaacagga tgtaaatgtg
     5881 cctgcttcga tgcatcattc tatagagtgg atcgagccca tcggtatgaa gctgctccca
     5941 tttcatgaac gcccccgtgg aaacaaaaac aagtagaagt gcgatgccga cggccagatg
     6001 aagtttcttc acaatgagtg atcgcttttc atgatgatgc tgtcaagggg cttctctctc
     6061 aagcacgggc cgcaacgttc gaggcacact agtcgaccgg taggtccttc gcgcaacgga
     6121 aaccttgatt gttgaaaggg gccactggag ctgtgtgtcc gcggtagtac aatccaaaac
     6181 cattgcacgt attttcggag caccaccacg atccaccgcg cgccactctc tcgtatccgt
     6241 gatcgggacc ttttggattt gcaaccgcac gatctggatt ctgatccgcc tccgatgctg
     6301 catcctcgta tgcgtaagga ctataatagt cagccgtcaa ttgccacacg tttccgactg
     6361 gatcgtacat cccccaatca tttggcggat acgcgcgcac tggagaaaga tacagatatc
     6421 catcttctag actgccgtgt tcgtgatgct ctgtctgcca gaagttcaat ccaaacgctc
     6481 cctttcgcat gggcgaatcg ccccagggaa atctgagatc gcttcctgct cttgcggcgt
     6541 attcccattc cgcttccgtc ggcaatctct tgccgaccat tgcacaatat cgatttgcat
     6601 cttcccatga aacagcggtc acaggatcct cttcgtgcac aggaacattg cgaagctctc
     6661 caaatggatt tttccagctg gctcctctgg cttgcttcca tttccagtct gccatccctt
     6721 ccaatgaaat catcgcataa cccatgcgtt cggcggacgt ttgatagcct gtgcgttcta
     6781 cgaacctgcg aaaatcagca acggtaacca gagtctcatc cagatagaac gaacgaattc
     6841 tcactttgtg gcgcggcctc tggtctgctt tgcgcgatat ctgcaagcct ctgaaatagg
     6901 ttcccgatgg aagtttcgcc atgcccgcgg gcgtttgctg gcagcttacg agaggaagga
     6961 atagaatccc gaggacgatt ctaaggaacg tctccattcg gatactcgac catcattcaa
     7021 agaaagaagg gccattgcat tgtgcgggcc cttgactttg ccaggaattc atcagcaatt
     7081 gctcgcaata agggttgtcc actttttcgg cggcatccag gaaagcctga cgcgtagcct
     7141 cgtcattgat tccgcgtttg tcataaccgg gaattcgttc gatcaccaat tgatggatgc
     7201 gctctcgggc attttgatcg cagcctgcgc gcaattctac gcgtctccaa tcaatcttca
     7261 aatgctcaca ggcgtcgcag ttctggtaaa attgccctgc tttctgcgct tcttccatcc
     7321 tgcgcagctc ttctcgcgcg aggctttcta catacgcccg attttctttt tcaatttcgt
     7381 tgatctgctc tctggtgtag acaaggagcg gaggctccct ggagacgaaa cctccaaata
     7441 catatccagt tcctgccgga ccggaatact tcaaccagcg agatgaaata ctatcgatgg
     7501 ttacgggggc acctgcgacg ccagaaaaat gaaaatacgc tccctttttc agaagggcca
     7561 caggtttgct cttcgcatct gcttccttgc gcacgtacag tccagatggc gccgtcacgt
     7621 agagaaactg tggaatggac agatcgtcaa tctcgctggt tgtgatggcg gagtgccctg
     7681 aagagcgaaa agaaatggaa tcaggaagat ttttgaacga cggtgcatta ctctgccaat
     7741 gagagcaatt ccccgctgga aatgtgaact gtcaatacaa gttaaactac atttcctgta
     7801 aacaggaggt acaccgtaaa caaaaagaca atcccaaatt tcatc
//
LOCUS       DAOQJL010000124         7809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5082_length_7809_cov_4.338406, whole genome shotgun sequence.
ACCESSION   DAOQJL010000124 DAOQJL010000000
VERSION     DAOQJL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_7809_cov_4.338406"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLG78_07300"
     CDS             <1..911
                     /locus_tag="PLG78_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22955.1"
                     /translation="KAFWDVIASERQVYCSQPYSVSLENDTCFSTGGLINYQEFMNGR
                     GALYPSRDVGPGVYLHAGIFVRAIATGHSKLAGAIQQTAFDNNVVINATNITSLVQYD
                     PNLNDGLKQVLPPYWFPLHFNSIMGGVTANSMYMTNDYVTRVVEMRFNMKENMMVHGF
                     TDTNGYRSIVAFSDWRRAHAQERDLGGNVLMRARVYYPDYVNTLVIQGGTKSNRHYYA
                     IVDPVETYIEDNLPVAATPVRQGTNIIKNLMGGVNYIIQCRFDANNDGYPETILDQQT
                     FGVLPGPGTVTIGCQCGGGVTLTLCH"
     gene            complement(937..1512)
                     /locus_tag="PLG78_07305"
     CDS             complement(937..1512)
                     /locus_tag="PLG78_07305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE22956.1"
                     /translation="MIKPLPSQTARDWHHPNCYGCGPDNAKGLHADFPFHNETGEVRF
                     QHSVEKSFEGAPGFVHGGVIAALLDEAQGTLCFHVGHFVMTEQLHMKYHRAMPLETQV
                     SVRCWLTAVRKRRLYTRGTIHSPSGELLASSSASWYVLTERILQKLFAERYSPEDHAR
                     NAAILEANRKRAREIRRRLRQVVSASNQAGF"
     gene            1564..2211
                     /locus_tag="PLG78_07310"
     CDS             1564..2211
                     /locus_tag="PLG78_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE22957.1"
                     /translation="MDLDIVSFAVAPLGCNCSILRETATNETIVVDPGGDADRIIDLL
                     TEKGCKVKYIVHTHAHFDHVLGTHEVASHCRNSGHASAVCMHPGDLPLYRILPQQCGW
                     FGLPPVEAKEEISHRLEDDEVLSFGRFKMHVLHTPGHTPGSCSFSIAERGILFSGDTL
                     FAGSIGRTDLPGGDYDQIIKSIKDRLMVLDDGTTVIPGHGEFTRIFDEKRTNPFL"
     gene            2218..3600
                     /locus_tag="PLG78_07315"
     CDS             2218..3600
                     /locus_tag="PLG78_07315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE22958.1"
                     /translation="MLFHTLDFALFFGLVLAAYHLAPTSFRKNLLLGASLFFYGYWNW
                     RILGLLAVTVTANYICALQLEKTRKRIWLIVAICVNLGLLGFFKYYGFFAESLSLLLG
                     RMNPGLPALNVLLPVGISFYTFELISYVVDVSRGLPAEKNFRDLLLFGTYFPKMMAGP
                     IARAGHLIPLLKDPPKSTLSGIQSGSILAFSGLFKKLVIADNLALEVNRLVGNHGAPM
                     SALDIGKHTISDAPTLILAATFFAVQIYADFSGYSDMARGMSRMLGIELVQNFNHPFR
                     ATNPAEFWRRWHITLGSFLRDYIYIPLGGNRSGMGMQMRNLLIVWTIGGLWHGASAGY
                     VAWGFYCGLCLCVYVLLRPFLSGGGPIVRGAGVLATFLTFALGLMIFRLGSPLEVARL
                     LSIWKAPEIHPLAFLFCLPVFVSHINGDNQSILEKLSPRAKWACLIAMFYVLIFLGRF
                     SGDEFFYFQF"
     gene            3575..4255
                     /locus_tag="PLG78_07320"
     CDS             3575..4255
                     /locus_tag="PLG78_07320"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNE22959.1"
                     /translation="MSSSTSSFDSEEMLKRLLGNSDRAPERERMVTEQIQRRGVKDPR
                     VLRAMRAIPRHAFIPGNLQSEAYEDHPVSIGEGQTISQPYIVAYMTELLEVKPGHSIL
                     EIGTGCGYQTAVLAILGAEVYTIEIIASLQQTARQTLHALGFENIDFYCGDGSSGWPQ
                     KRTFDRIIVTAAPPTIPQELVNQLAESGKMVIPVGEMQQDLTVMRRSNGRLSEEKVLP
                     VRFVPITH"
     gene            4339..5256
                     /locus_tag="PLG78_07325"
     CDS             4339..5256
                     /locus_tag="PLG78_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNE22960.1"
                     /translation="MGRSLVILAFLLCSSLSAEIVYMKDGRILEGSIVAQNDQTLDIK
                     TKNGLQKIQKSTVRRIAYHTAEEKAKLEAERKARRDAAMKELERMRQLRAQEEEARKR
                     ADIARQAELAKQRAAAAKAVRESVEREDMEKPDDIIGFKDFAWRSALVPGWGHFAIGR
                     PIVGSIYAGATAGALYNIYDKRRKALAARDANHNDVLVNLLVSVAPVDAPLPFRLAYG
                     LDANRRAFTEYRARINRYNYSIASLGIVYGVQMTHIILNGITWESGFIVDSGVRPTFD
                     ISYNEHNTDERSFNREPDKQIVASFRMTF"
     gene            5301..6527
                     /locus_tag="PLG78_07330"
     CDS             5301..6527
                     /locus_tag="PLG78_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE22961.1"
                     /translation="MAIPPLDISKKIVDFFEKATVGTLSGIRDILDTSMGSTANRLSE
                     LTDKPLVKFTEIQGAFQKAAGQLSTANQVTAFGLATAIQSVTDAFDSAGNALRIADEF
                     THKHIFENVHISSTAGTSFDALLKTKIVASFRLRDADCSAQQIAEAVKASGKTSAVLF
                     VPGLFCDDGMWTQGPEPLLPLIESMGYASVLIRLHPGLPISENGQKLVTLLDDLFANI
                     EIPLHIISFSNGGLILRSCLYYAKLAGKPWLKHLRKVAIISPPDGGSYIEKLGFWLGL
                     ALKSTPSLGLQLLGWVGHMRSDAMKDLSHGIIRQEDRHELSQIKRYGVDLYYNELDDL
                     DAYLIYSLISESNDPVKTWLGDGVCEKKSLEYLRESVYLKKPNPEKRVRVIAQRSHFQ
                     ILSSAELRMHLKEIFS"
     gene            6524..6865
                     /locus_tag="PLG78_07335"
     CDS             6524..6865
                     /locus_tag="PLG78_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNE22962.1"
                     /translation="MKDKTIFAKIIDREIPASIVYEDDQCLAFKDVHPQAPVHILIVP
                     KKPIDRLSSASGEDQLLLGHLLLTANKIAEQAGLKDFRLVVNSGPGAGQTVLHLHVHI
                     LGGREMEWPPG"
     gene            complement(6911..7225)
                     /locus_tag="PLG78_07340"
     CDS             complement(6911..7225)
                     /locus_tag="PLG78_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich protein"
                     /protein_id="HNE22963.1"
                     /translation="MRSILYSTLVVVLLALGFSQAIAQPATCEAACAKYRECAVDIWK
                     KNNKVPTAEQSKTMYGGCMRTCGKFKEKTLVCYKQTLAAKGDACMTYWSCVQSAYNSG
                     KK"
     gene            7456..>7809
                     /locus_tag="PLG78_07345"
     CDS             7456..>7809
                     /locus_tag="PLG78_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNE22964.1"
                     /translation="MAKSPSEVLNWAKSEKVEFVDIRFTDMFGTMQHFTMPLHAIDEE
                     SFTYGIGFDGSSIRGWKAINESDMLARLDADTVFIDPFLEHKTMAVVCDIYEPGANPK
                     PYGRDPRYILKKAIKY"
BASE COUNT         1962 a         2007 c         1948 g         1892 t
ORIGIN      
        1 cgaaggcttt ctgggatgta atcgcatctg aacgccaggt ctactgcagt cagccttact
       61 ctgtaagcct cgaaaatgat acctgcttct ctacgggcgg actcatcaac tatcaggaat
      121 ttatgaacgg ccgcggtgcg ctctaccctt caagagacgt cgggcccggt gtatatctcc
      181 atgcaggaat ttttgttcgc gcaatcgcaa ccggccatag caaactggcg ggcgcaattc
      241 aacaaactgc gttcgacaac aacgttgtaa tcaacgcaac caacatcact tcgcttgttc
      301 agtacgatcc caatctgaac gatggtctga aacaggttct gccgccgtac tggtttcctc
      361 tccatttcaa ctctatcatg ggcggcgtaa ctgctaactc tatgtacatg actaacgatt
      421 acgtaacaag agtcgttgaa atgcgtttca acatgaaaga aaatatgatg gttcatgggt
      481 ttacggatac aaatggctat cgctccattg ttgcattcag tgactggaga agagcacatg
      541 cgcaggaacg cgatctgggt ggcaatgtgc tcatgcgcgc gcgcgtttat tatccggact
      601 atgtgaacac tctcgtcatt cagggcggaa caaagagcaa tcggcactac tatgcaatcg
      661 tggatccggt tgaaacttat attgaggaca atcttccagt ggcggcgacg ccggtacgac
      721 agggtaccaa catcatcaag aacctgatgg gtggagtgaa ctacatcatc cagtgcagat
      781 ttgatgcgaa taatgacggg tatcctgaaa ccattctgga ccagcagacc ttcggtgttc
      841 tacccggtcc agggacagtt actattggat gccagtgcgg cggcggggtc actctcactc
      901 tctgccattg acctctgcgg ttgactattt tcgcgatcag aatcctgcct gatttgatgc
      961 agaaacgacc tgacgcaggc gacggcgaat ttcgcgagct cgtttgcggt tggcctcaag
     1021 aatcgccgca ttgcgcgcat gatcttcggg cgaatatctc tccgcaaaca acttctgcaa
     1081 tattcgctca gtaagaacat accaggacgc gctggaactg gccagaagct ctccggaagg
     1141 ggaatggatg gtaccgcgtg tgtagagcct tctctttcta actgcagtca gccagcagcg
     1201 aacggaaacc tgagtctcca gaggcatcgc gcgatggtac ttcatgtgaa gttgctccgt
     1261 catcacaaaa tggcccacat gaaagcacaa tgtaccctgc gcctcatcta aaagggcggc
     1321 aatgacgcca ccgtgcacaa atccaggcgc gccttcaaaa gatttttcca cactgtgctg
     1381 aaatctcact tctcccgttt cgttatgaaa tggaaaatct gcgtgtagtc ctttcgcatt
     1441 atcgggccca cagccgtagc agtttgggtg atgccagtca cgcgctgtct gactgggaag
     1501 cggtttgatc atgtgtcaga tttcagaata gacagccggc gcgcatccaa tctttatcat
     1561 cgagtggatc ttgatatagt gagttttgca gtcgctcctc ttggatgcaa ctgctccatt
     1621 ctgcgggaaa cggcaaccaa cgaaaccata gtcgtggacc cgggcggaga tgcggatcga
     1681 atcattgatc tgctcacaga gaagggctgc aaggttaaat acatcgttca tactcacgct
     1741 catttcgatc acgttcttgg aacgcatgaa gtcgcttctc attgcagaaa ttcaggacac
     1801 gcatccgcgg tatgtatgca tccgggggat ctccctctgt atcgaattct gccccagcag
     1861 tgcggctggt ttggtctacc tcctgtcgaa gcaaaagaag aaatatcaca cagactggag
     1921 gatgatgaag tgttgtcgtt cggtagattc aaaatgcatg tcttgcacac gcccggacac
     1981 actcctggat cctgctcctt tagcatcgca gagcgcggaa tcctcttcag cggggacacg
     2041 ttattcgcag gaagcatagg caggaccgat ttgcctggcg gcgactacga ccaaatcata
     2101 aaaagcataa aagatcgcct catggtacta gacgacggaa ccactgtgat ccccggccac
     2161 ggagaattca cgcggatctt tgatgaaaaa agaacaaatc cctttctcta atctactgtg
     2221 ctatttcaca ctttagactt cgcgctgttc tttggtctgg tcctggccgc ttaccatctg
     2281 gcccctacga gcttccgaaa gaatttactc ctgggtgctt ctctgttttt ttacgggtac
     2341 tggaactggc gtatactagg cctgcttgca gtaacggtaa ctgcaaacta catctgcgcg
     2401 ctacaattgg agaaaacgcg caaaagaatc tggctgatcg tagccatctg cgtcaatctc
     2461 ggactacttg gcttttttaa gtattacggt ttctttgcag agagtctgtc cttacttctg
     2521 ggccgaatga atccaggcct gcccgcactg aacgtacttt tgccagtcgg gattagcttc
     2581 tatacttttg aattgatttc ctacgtagtt gatgtgagcc gcggactacc cgcggagaag
     2641 aacttccgcg atctacttct atttgggact tactttccaa aaatgatggc cggcccgatc
     2701 gcaagggccg gtcatttgat tcctctttta aaggatcctc caaaatcaac gttaagcggt
     2761 attcagagtg gttccatcct cgcattcagc gggcttttca agaagctagt catcgcggac
     2821 aacctggcac tggaagtaaa cagactggtg ggaaaccacg gagctcccat gagtgcactc
     2881 gacatcggga agcacacaat ttctgacgca cccaccctga tactggccgc aacttttttc
     2941 gcggttcaga tttatgcaga cttctccggg tattcagaca tggcccgggg tatgtctcga
     3001 atgctgggaa tcgaacttgt ccaaaacttc aatcatccat ttcgcgcaac caaccccgcc
     3061 gaattctgga gacgatggca catcactctg ggctcctttc tcagagatta catatacatt
     3121 ccgctcggtg gaaatcgatc ggggatggga atgcagatgc gaaatctcct cattgtctgg
     3181 acgattggtg gactgtggca tggggcttcc gccggctacg tggcgtgggg attctattgc
     3241 gggctgtgcc tgtgcgtgta tgtccttctg cgtccttttc tttcaggcgg aggcccgatc
     3301 gttcgtgggg caggagttct tgctacattt ctgacttttg cactgggcct gatgatcttt
     3361 cgcctgggtt cgcccctgga agtggcacga ctgctaagca tatggaaagc cccagaaatt
     3421 cacccgcttg ctttcttatt ctgtcttcca gtatttgttt cgcacattaa tggagacaat
     3481 cagagcattc tggaaaagct ttctcctcgc gcaaaatggg cttgcctgat tgctatgttt
     3541 tacgtattaa tctttttggg cagattttca ggagatgagt tcttctactt ccagttttga
     3601 ttcagaggaa atgctcaaac ggcttttggg caattcagat cgcgccccgg aacgagaacg
     3661 catggtcacc gaacagatcc agagaagagg cgtaaaggat cctcgcgttt tgcgagctat
     3721 gcgagccatc ccgcgacacg cattcattcc tgggaatctg caaagcgaag cgtacgaaga
     3781 tcatcctgtg tccatcgggg aaggacaaac aatttcgcag ccatacatcg tggcgtacat
     3841 gacagaactt ctagaagtta aaccaggaca ttcgattctt gaaattggaa caggttgcgg
     3901 ctaccagacg gcagtgcttg ccattctggg cgcagaagtg tatacaatcg aaatcattgc
     3961 cagcctgcaa cagacggccc ggcagacgct gcatgctctt ggattcgaga acattgattt
     4021 ctattgcggg gatggaagct caggctggcc acagaaacga acgtttgatc gaatcatagt
     4081 cacagctgct ccaccaacca ttccacaaga attggtgaat caactggcag aatccggaaa
     4141 gatggtgatt ccggtggggg aaatgcaaca agatcttacc gtcatgcgtc gatcaaacgg
     4201 gcgtctgtcg gaagaaaaag tcctgccggt aagatttgtt cctatcacac actgacccat
     4261 tctaaagaac tcgtgcagat cttcaaattt tcaacggaag cagtttgacg gccccgggtc
     4321 gtggtcagag tttgtgccat gggccgcagc ctcgtcattc ttgccttcct actgtgctct
     4381 tctctaagcg cagaaatcgt atatatgaag gacggccgca ttctcgaagg ttccatcgtg
     4441 gcccagaacg atcagaccct ggatattaaa acaaaaaacg gacttcagaa gattcaaaag
     4501 tccaccgtgc gaagaattgc atatcacacg gcggaagaga aagcaaaact cgaagcggaa
     4561 cggaaggcca gacgagacgc ggccatgaaa gaactggagc gcatgagaca actgcgcgcg
     4621 caggaagaag aagcacgtaa aagagcggac atcgctcggc aggcagaact ggccaaacag
     4681 cgcgccgctg cggccaaagc tgtacgcgaa agtgttgagc gcgaagatat ggagaaacca
     4741 gacgatatca ttggtttcaa agacttcgcg tggaggtctg ctctggttcc aggatggggt
     4801 catttcgcaa tagggagacc gattgttgga tccatctatg caggcgcaac ggccggtgca
     4861 ctctacaaca tctatgacaa aaggcgcaag gcgctggccg cacgcgatgc gaaccacaac
     4921 gacgttctgg tcaaccttct cgtttctgtg gcacctgtgg acgctcctct tccgttccga
     4981 cttgcctacg ggttagacgc aaaccgcagg gctttcaccg agtacagggc acgcatcaat
     5041 cgctacaatt actccattgc ttccctcgga attgtctacg gagtgcagat gacccacatt
     5101 atcctgaacg gtatcacgtg ggaaagtggt ttcattgtgg acagtggggt tcgccctacc
     5161 tttgacattt cgtataatga gcataatacg gacgaacgat ccttcaaccg cgagccggac
     5221 aaacaaatag tcgcgtcttt tagaatgacc ttctaaagtt attccttgaa cttcaccgtg
     5281 ggcgtgagat aaaaggaccc atggccatcc ctccactgga cataagcaaa aagatcgtag
     5341 acttctttga aaaagcaacg gtcggaacgt taagcggcat tcgcgatatc ctggatacct
     5401 ccatgggtag caccgcaaat cgtttgagcg agctcactga caagccgctc gttaagttca
     5461 cagaaatcca gggcgcgttt caaaaagctg caggccagct cagcactgcc aaccaggtaa
     5521 ctgctttcgg gctcgcaaca gccattcaat ccgtcacaga tgcattcgat agcgccggaa
     5581 atgctcttcg catcgcagat gaattcacgc acaaacatat ctttgaaaat gtgcatatca
     5641 gcagcaccgc cgggacttct tttgatgcgc tactgaagac caagattgtt gcgtcctttc
     5701 gcttgcgaga tgctgattgc agcgcccagc agatcgcaga ggcagtcaaa gcttcgggaa
     5761 agaccagcgc cgttcttttt gttccggggc ttttctgtga tgatggaatg tggactcagg
     5821 gaccagagcc cctcctccca ttgatcgaat ccatgggata tgcatccgta ttgattcgtc
     5881 tgcacccagg gcttcccatt tcggagaacg gacaaaagct cgttacactc ctggatgatc
     5941 tgtttgcgaa catcgagatc ccgttgcaca tcatcagctt cagcaacggc ggcctcattc
     6001 tacgcagctg tctatactac gcgaagctgg cgggcaaacc atggttgaag catttgcgca
     6061 aggtcgccat catcagccct ccagacggcg gttcctatat tgaaaaactt ggtttctggc
     6121 tgggccttgc gctgaagtcg actccctctc ttggtctgca actgctcggc tgggttggtc
     6181 atatgcgcag cgatgcgatg aaggatcttt cgcatgggat cattcgccag gaagacagac
     6241 atgaactcag ccagatcaaa aggtacggtg tggatctata ctacaatgaa ctggatgatc
     6301 tggacgcgta tctgatctac agcctcatat cagaaagcaa tgatccggtg aagacgtggc
     6361 tgggagacgg agtttgcgaa aagaaaagcc ttgagtatct tcgtgagtca gtgtacttga
     6421 aaaaaccaaa tccagaaaaa cgggtgcggg tcatcgcgca aagatctcat tttcaaattc
     6481 tatcttctgc agaactgagg atgcacctca aggaaatctt ctcgtgaaag ataaaaccat
     6541 cttcgcaaaa atcatagata gagaaattcc cgcgtcgatc gtctacgaag acgatcaatg
     6601 tctggccttc aaagatgttc atccccaggc accggttcac atactgatcg ttcccaaaaa
     6661 gccaatcgac cggcttagct ctgcttcagg cgaagatcaa ttgctccttg gccatctact
     6721 tctgaccgcg aacaaaatcg cagagcaggc ggggctaaaa gatttcagac tggtagtcaa
     6781 cagcggtccc ggggccgggc agactgtgct tcacttgcat gtgcatatac tgggtggacg
     6841 cgagatggaa tggccgcccg gttagggcga ccattttgat ttatttgtag aactttagtt
     6901 tctggctgcc ttacttcttc ccgctgttgt aggcagactg aacgcagctc cagtaggtca
     6961 tacaagcgtc gcctttggcg gccagagtct gcttgtagca aaccagagtc ttctctttga
     7021 actttccgca agtgcgcatg cacccgccgt acatcgtttt actctgctcg gcggtcggaa
     7081 ccttgttgtt ctttttccag atgtcgacag cacattcacg atacttcgcg caagcggcct
     7141 cgcaggtcgc aggctgtgca atcgcctgac tgaaacccag agcaagcagg accacaacca
     7201 gagtgctgta aagaatcgat ctcattttcc ctccggggca attccaaatg ccacctgtat
     7261 agtagacggt ccccccgtcc attcgttcgc gcaaaaccgg ctttcaagcc gtgcctggac
     7321 cagcgacagg ttgccgtagc ctatgccgaa tgccaaaaaa tcaggcaaaa aaatgccatt
     7381 tccgggggta cattacttgc tcttatacgc atgagcggct aaccatgcgg cgaaccaaat
     7441 atcaggagtc tttgaatggc taaatcacca tcagaagtcc tcaactgggc caagtcggaa
     7501 aaggtcgagt ttgtggacat tcgttttacc gacatgttcg gaaccatgca acatttcacg
     7561 atgccgctac acgcgatcga cgaagagtct ttcacatacg ggatcggttt tgatggttcc
     7621 tccatccgcg gatggaaagc aatcaacgaa tcagacatgc tggctcgtct ggacgcagat
     7681 acggtgttta ttgatccgtt tctcgagcac aagacgatgg cagtcgtatg cgacatttac
     7741 gaaccaggag cgaatccgaa accttacggc cgcgatcctc gttacattct aaaaaaagcg
     7801 atcaaatac
//
LOCUS       DAOQJL010000125         7782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5109_length_7782_cov_4.893749, whole genome shotgun sequence.
ACCESSION   DAOQJL010000125 DAOQJL010000000
VERSION     DAOQJL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5109_length_7782_cov_4.893749"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PLG78_07350"
     CDS             <1..1347
                     /locus_tag="PLG78_07350"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE22965.1"
                     /translation="AEIEQDSFRFSLWKLTDAKYREAAEQYYERKSKELHYVDPNRGL
                     PSRLKRPPVRHWKYRELPEIDREFWKYRIRLAGNLLKKYANIKNSRFEFNCHHRQSVL
                     VNSEGSMILQQQCIFELRGSLWLLNKKGEGIVQEIHFTEGDIKDLPDEKEFLRIIREK
                     IDRLLILEKAPRMDSYSGPVLLSPVAAGLFFHEVVGHRLEGSRLLSPDEGGTFRSLRG
                     KRIAPDYIDIIDHPSLTAFGNRQMIGHFLYDDEGSPAEKSVLVEKGILKEFLTTSTPI
                     PGQKKTNGHARSQKFERPISRMGNLMVVNRKPVSPEEMKERFIEEIRRQKKPYGIHVL
                     DTLGGETGTNSYDFQAFKGEILDATRVFPDGREEPVRGVDFVGTPLSSLDSVMCMGND
                     PMLDNSYCGAESGVVPVSTVSPSMLMRSLELQGQDRERYTQYVVPLPYSTGKRKKN"
     gene            complement(1348..2739)
                     /locus_tag="PLG78_07355"
     CDS             complement(1348..2739)
                     /locus_tag="PLG78_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE22966.1"
                     /translation="MALNHRERIFHLLLVEYGRILQRELMKHPSTLDRPMRDGRSYRA
                     FLETQLAETESITGPVRSGPSILESIYVRFVDKSVLPAQDFDTVTAKNYILAIRSRME
                     AIQAHDEQEEERRIRQSVDIMMRVVNSANTTFTEFNLRENAVRIELLLSLKKELGEEA
                     AIYKEARSMMDRHSLILINYMHELFLDRLKGALEAEQKLREELEKKRQQTEQELRMAQ
                     KIQESLLPARFPEDASLRFAARYLPMSSVGGDFYDAQSISLADQSTGVGVIIADASGH
                     GVPAAFIAAMTKMIWPGSFAGDSDPARALTRMNDSLYERISGNFITVSLGFFQPLDQP
                     PQSLAPSKRGVFHFANGGHQPPVLLRKGESPSLLEAKGRIVGIFPEMSVEARCIDYQA
                     GDRFVFYTDGLVEARKNSGEMLGDESVIAYMDSLRNLPPDEFCDRLIGYVRESTSNCA
                     QDDDVTLFVIDVV"
     gene            complement(2739..2987)
                     /locus_tag="PLG78_07360"
     CDS             complement(2739..2987)
                     /locus_tag="PLG78_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNE22967.1"
                     /translation="MSFKPVIAEKAPFVCEEKQGTTKFWCACGLSQNQPYCDGSHKRE
                     KTGMSPVKVEFSEDKKVAWCRCKQTKTPPFCDGSHRSC"
     gene            3102..3305
                     /locus_tag="PLG78_07365"
     CDS             3102..3305
                     /locus_tag="PLG78_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE22968.1"
                     /translation="MKQNMGTVDRIIRIVAALAIAVLAYVNLISGTVAIVLGVLAVVF
                     IATSFVGFCPLYLPFGLSTRPKE"
     gene            complement(3316..4065)
                     /locus_tag="PLG78_07370"
     CDS             complement(3316..4065)
                     /locus_tag="PLG78_07370"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE22969.1"
                     /translation="MLDSEAMDQIKVAASQLASASSLAVITGAGISAESGIPTFRGEG
                     GLWRKFRAEDLATPEAFRRDPNLVWEWYDWRRQICARALPNAAHEALALLESRMSSFL
                     LITQNVDGLHQRAGSKRILEIHGNIWKARCTACKTKFEISETPLPSLPVLCPDCGSLA
                     RPHIVWFGESYDESLIQEAVSFLRQCDVLLVIGTSGMVSMPGFLTEQAFRAQRIELNP
                     EPSGITPLVQISIRAKAAEAMPLFWERLNAG"
     gene            4069..5271
                     /gene="thiH"
                     /locus_tag="PLG78_07375"
     CDS             4069..5271
                     /gene="thiH"
                     /locus_tag="PLG78_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNE22970.1"
                     /translation="MIASRFVKTDHPPARRNYLMGLPNPPGFPGIVFTDYYRRMHFAS
                     ISAEIENASSSDVEHALSKAGTGQISDRNILALLSPAALPYLEEMARMAQSITRQRHG
                     NIIQMYAPLYLSNECRSSCTYCGFSYENNIRRRTLSMEEIEKEGSILYDQGIRHVLLL
                     TGEDYKRTPVQFIADAARRLAKSFSSVSIEVYPLKEDDYSMMREAGVDGLAVYQETYD
                     PECYRRVHLRGMKKNLEFRLECPDRAASAGIRKISIGALLGLSDSATEVFFCAQHARY
                     LMKTYWQTQLSVSLPRLRPAEGLTDVPLVKNRSYVQYLCALRIILPDAGIILSTREGE
                     SFRDQMAGICITQMSAGSRTEPGGYSGLEATVQFETEDRRSVHEFCSSLRANGLEPVF
                     TDWSPVMK"
     gene            5306..5827
                     /gene="hpt"
                     /locus_tag="PLG78_07380"
     CDS             5306..5827
                     /gene="hpt"
                     /locus_tag="PLG78_07380"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNE22971.1"
                     /translation="MQSDFEILISGKQIAEKVREIGAQITRDFQNRELVIIGVLRGCF
                     VFMADLARAIDLPVAIDFMEVSSYGDDLESSGNVKIIKDIKDPIHGKHVLLIEDIIDT
                     GHTLNFVVDHLKLKNPASIGIAGLLIKKDKHNFRGKIDYSGFDIADHFVVGYGMDAKG
                     LYRNLDHVAIFRA"
     gene            5857..7368
                     /locus_tag="PLG78_07385"
     CDS             5857..7368
                     /locus_tag="PLG78_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22972.1"
                     /translation="MNIPASTRAFKLPAIVILSLAALTFFAAVRSMPVETSVFADGIG
                     KWLQIRGLLTRGDFECAYQESVDPDFQFIPGPWYFYTIQNGKCSYGYQAAYALLAAPF
                     FKIHPLGFLFLHFFLYLAMILGFMLCAHRLLPERSWAPWWAGIIAAWIIPTVVFAMDL
                     SEVILSLAMGAWALALLCKRESFQSIEWKLPSFSEFKYFNSVIFASGIVLGLILSLRT
                     ESLIHGICWAFGLLLVQRKSFVSFASGYAVAILAVMGFHLWFFGNLMGNRGSSHAAVT
                     AGLTASTHIGIARTLLLGGPLGLITSLPAIFLGLFAFSRHARKIHGPQILFMAVISIL
                     PTLGVLITAPSDGGYSWGPRFLSLTFAPYFLLVLLALDHPYAHRWPAMIVLAAIAAYG
                     MQYTWKGRNVYRRAMVQNAGYAAYIESTSSEAIIGADYSLLAILSRSALQGHVYQARS
                     IPEITILADRLKNAGVRRVILLRYATSEVTQLPGWTVKSRKSMDLVEFVVCER"
     gene            complement(7330..>7782)
                     /locus_tag="PLG78_07390"
     CDS             complement(7330..>7782)
                     /locus_tag="PLG78_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE22973.1"
                     /translation="ADEPTTALDVTVQAQILELMNALQKEMGTAILLITHDLGVVAQT
                     AHTMSVLYAGQVVESGSVRDVFSEPCHPYTQALFQSLPARARNGELVPIPGSVPPATE
                     YDSLHSPCRFYDRCQFKDDRCFQQSTAPGHVAWCRRSAFANNKLNQVH"
BASE COUNT         1955 a         1976 c         1929 g         1922 t
ORIGIN      
        1 gcggagattg agcaagattc gtttcgcttc agcctttgga aactcacgga tgcaaaatat
       61 agagaagcgg cagagcagta ttacgaacgc aaatcaaaag aactccacta tgtggatcca
      121 aacagaggtt tgccatcgcg tctgaaaaga ccgcccgttc gccactggaa gtacagagaa
      181 ctccctgaaa tagacagaga attctggaag tacagaattc gactcgcggg caaccttctc
      241 aaaaaatatg cgaacattaa gaactctcgt tttgaattca attgtcatca cagacagagt
      301 gttctcgtaa attcagaagg ttccatgatt ctgcagcagc aatgcatttt cgagctgcgc
      361 ggttcactgt ggcttttgaa taagaaggga gaagggattg ttcaagaaat ccacttcaca
      421 gagggagaca tcaaagacct cccggatgaa aaagaatttc ttagaatcat tcgagagaaa
      481 atcgatcgcc tgctgattct tgaaaaagcc cctcgcatgg attcctattc aggtccggtt
      541 ctactttcgc ctgtggccgc cggcctgttc tttcacgaag ttgtgggaca cagactcgaa
      601 ggctctcgtc tcctttctcc ggatgaagga ggaactttca gaagtctccg cggcaagaga
      661 atcgcgccag attatataga tatcattgac catccttctc tcactgcatt tggaaacaga
      721 cagatgattg gtcatttcct ctacgacgac gagggttccc cggcggaaaa atctgtgctc
      781 gtggaaaaag gaattctgaa ggagtttcta acgacttcca ctccgattcc agggcagaag
      841 aaaaccaacg gtcacgcaag aagccagaag tttgagcgtc ccatttcgcg catgggcaat
      901 ctaatggtgg tcaatcgcaa gccagtttct ccggaagaaa tgaaagaacg ttttatagaa
      961 gaaatccgca gacaaaagaa accgtatggc attcacgttc tggacactct gggtggagaa
     1021 acgggcacaa acagctacga ctttcaggct ttcaaaggag agatcctgga tgcaaccaga
     1081 gtattcccgg acggcagaga agaacctgtc cgcggagtgg attttgtggg gactcccctt
     1141 tcctctctgg attcagtcat gtgcatgggg aacgacccaa tgctcgacaa cagctattgt
     1201 ggcgcagaat caggagtggt cccggtttca acggtgtctc cctccatgtt gatgcgaagc
     1261 ctcgagcttc agggacagga cagagagcgc tacactcagt atgttgtgcc gctgccgtat
     1321 tctactggaa aaagaaagaa aaactgatca gactacatca atgacgaata acgtcacatc
     1381 gtcgtcctgg gcgcagttgc tagtgctttc tctcacgtat ccgatcaacc tgtcgcaaaa
     1441 ctcatccgga ggaagatttc gcaaggaatc catgtacgcg atcacggact cgtctccgag
     1501 catctctcca gaattcttcc tggcttctac cagaccgtct gtgtaaaaaa caaatcgatc
     1561 cccggcctgg tagtcgatgc atcgcgcttc cacggacatc tccggaaaaa tgccgacgat
     1621 acggcctttc gcttcaagca aggaaggaga ctcacctttc ctgagtaaaa ccggtggctg
     1681 gtgtccacca tttgcaaaat gaaaaactcc gcgcttgctt ggagcaagac tttgcggcgg
     1741 ctgatccaga ggctgaaaga atccaaggct aactgtgatg aaatttcctg aaatgcgttc
     1801 gtaaagactg tcattcattc gcgtaagcgc acgggcagga tcagaatctc ccgcgaaact
     1861 tcccggccag atcatctttg tcatcgcagc gatgaaagcc gctggcactc cgtgaccaga
     1921 agcgtcagcg ataatcacac ccacgcccgt cgattgatcc gcgagcgaaa tcgactgagc
     1981 gtcatagaaa tctccgccaa cactggacat gggcaaataa cgggccgcga agcgaaggct
     2041 ggcatcttct ggaaaacgag caggcaacag tgattcctgg atcttctggg ccatgcgaag
     2101 ctcctgttct gtttgctgcc gtttcttctc caattcctca cgcagttttt gttccgcttc
     2161 gagcgcgccc ttcaggcgat cgagaaagag ctcgtgcata tagttgatca agatgagaga
     2221 atgtcgatcc atcatggatc ttgcttcttt gtaaatggcc gcctcttccc ccagttcttt
     2281 cttaagagaa agaagtagtt ctattcggac tgcgttttcg cgcagattga attcagtgaa
     2341 agtcgtgttg gcggaattca cgactcgcat cattatatct acggactggc ggatccttcg
     2401 ctcctcttct tgttcgtcat gtgcctgaat tgcttccatg cgagagcgga ttgccagtat
     2461 gtaattcttc gcagtgacag tgtcgaaatc ctgggcagga agaacgctct tatccacaaa
     2521 acgaacgtag atgctttcca gtatggacgg acctgaccgg accgggcctg taatggattc
     2581 cgtttccgcc agttgcgttt ccagaaatgc gcgataggac cgcccgtccc tcatggggcg
     2641 atccagagtg gacgggtgtt tcatcagttc cctctggagt attctaccgt actcaaccag
     2701 gagaagatga aaaatgcgtt ccctgtgatt caatgccatc agcagctgcg atgcgaaccg
     2761 tcacaaaatg gcggtgtctt tgtctgtttg catctgcacc atgccacttt cttgtcttcc
     2821 gaaaactcaa ctttcacagg agacatcccg gtcttttcgc gcttgtgact tccatcgcag
     2881 tagggctggt tttgcgaaag accacacgcg caccagaact tcgtggtacc ctgcttctct
     2941 tcgcatacga aaggggcttt ctctgcaatg accggcttaa acgacatgcc tcaaagtaag
     3001 aactacctga ccggacgtca atgggaatca ctgttaaaac atgacaaata tcatgtgcga
     3061 acccgacctg gatcgcctat cattggaaca tagaggatac tatgaaacaa aacatgggga
     3121 cagtggatcg aatcattcgg attgtagcag ctctcgccat cgcagttctt gcttacgtga
     3181 atctgatttc agggaccgtc gcaattgtac tgggagttct ggcagttgtt ttcatcgcta
     3241 cgagctttgt tggattctgc ccgctctacc ttcctttcgg actttctact cgtcctaagg
     3301 aatagaaact aaacgttagc cggcattgag cctctcccag aagagaggca ttgcctccgc
     3361 ggctttcgca cgaatcgaaa tctggaccag tggagtaatt ccggaaggct ccggattcag
     3421 ttcgattctt tgtgcgcgaa atgcctgctc tgtaagaaac ccgggcatag aaaccattcc
     3481 ggaagtgcct atcaccagta gaacatcgca ctgacgcaaa aaagaaactg cctcttgtat
     3541 caaagactca tcatacgatt ccccgaacca gacaatgtgc ggtctggcca gagatccaca
     3601 atccggacac aggactggaa gtgagggaag cggagtttcg gagatttcaa atttggtttt
     3661 gcatgcagtg cagcgcgctt tccagatgtt tccgtgaatt tccagaatgc gtttgctacc
     3721 agccctttga tggaggccgt ccacattctg agtgatcaga aggaaagaag acattctgga
     3781 ttcgagaagc gccagagcct cgtgcgccgc attgggcaga gcgcgcgcac agatttgacg
     3841 cctccaatca taccattccc agaccaaatt cggatcgcgc ctgaacgctt ctggagttgc
     3901 cagatcctcc gcacgaaatt ttcgccagag tccaccttca ccacgaaaag taggaatgcc
     3961 gctttctgca gaaatcccgg cccctgtgat cacagcaagg cttgacgcgc tcgccagctg
     4021 agaggcagcc actttgatct gatccattgc ttcggaatcc agcattcttt gatcgccagt
     4081 cgatttgtga aaacagacca cccaccagca aggcgaaatt atttgatggg tcttccgaat
     4141 ccgcctggtt ttcctggcat tgtgttcacc gattattata gaagaatgca cttcgcgtcc
     4201 atctccgcag agatcgaaaa cgcgtcttcc agcgacgtgg aacatgcact ctccaaagca
     4261 ggaacgggcc aaatttcaga tcgaaacatc ctggcgctcc tctctcccgc cgcccttccg
     4321 tatcttgagg agatggcccg gatggcacaa agcatcacac gccagagaca tggaaacatc
     4381 attcagatgt atgctccttt gtatctttcc aatgaatgca gatccagctg cacttactgc
     4441 ggcttcagct acgagaacaa catcagaaga agaactctca gcatggaaga aattgaaaag
     4501 gaggggagca ttctatacga tcagggaatt agacacgtgc tgcttttgac aggagaggat
     4561 tacaaacgca cacccgtgca attcattgca gacgcagcac gcagactcgc gaagagtttc
     4621 tcttcggtga gcattgaagt ctatcctctc aaagaagatg actattcgat gatgcgagag
     4681 gcaggagtcg acggcctggc agtgtatcag gaaacgtacg atccagaatg ctacaggcgt
     4741 gtgcatctgc gtgggatgaa aaagaatctg gagttcagac tggaatgtcc ggatcgagcg
     4801 gcgtctgcgg gaatcaggaa aatttccatc ggcgcgcttc taggactttc tgattcagcg
     4861 actgaggttt tcttttgcgc acagcacgcg cgctacttga tgaaaacata ctggcagact
     4921 caattgagtg tatcgttgcc caggctcagg ccagccgagg gacttacaga cgtccctctt
     4981 gtgaaaaatc gatcctatgt ccaatacctt tgcgcgcttc gcatcatttt gcccgatgct
     5041 ggaatcattc tgtccacgcg cgaaggtgaa tccttccgcg accagatggc aggaatctgc
     5101 atcactcaaa tgagcgcagg ttctcgcacg gaaccaggcg gatattccgg gctcgaggca
     5161 actgtgcaat ttgaaacgga agatcgcaga tccgtacatg aattctgcag ttcactgcgt
     5221 gccaatggac ttgaaccggt cttcactgac tggagcccgg tgatgaaata gtttccaccc
     5281 attgtgtggc tgatcaaact ggtttatgca gtcggatttt gaaatcctga tcagcggcaa
     5341 acagattgcg gagaaggtcc gggaaattgg ggcacaaatc acgcgcgact tccagaaccg
     5401 cgagcttgtg atcataggag tattgcgcgg ctgcttcgta tttatggcag atcttgcgcg
     5461 ggccatagat ctgccggtgg ccattgactt catggaagta tcttcctacg gagacgatct
     5521 cgaatcatcg ggaaacgtaa aaatcataaa agatatcaag gatccaatcc acggtaaaca
     5581 cgtactcttg attgaggaca tcattgatac cggccacacc cttaactttg tggtggatca
     5641 tctcaagctg aagaatccgg catccattgg aattgcgggc ctgctgatta aaaaggacaa
     5701 acacaacttt cgcggaaaga tcgactactc gggctttgac attgcagacc atttcgtagt
     5761 cggctacggc atggatgcga aaggactgta tcgcaatctt gatcatgtag cgatttttcg
     5821 cgcttgatct attggaattc caaccaacaa aggtgcgtga acatacccgc atccacgcgc
     5881 gctttcaagc ttccagcaat cgtaatcctg agtcttgctg ctctcacatt ctttgccgca
     5941 gtccgctcca tgcctgtaga gacgagtgtt tttgcagatg ggattggtaa atggcttcaa
     6001 atcagaggtc tcctcacgcg cggtgatttt gagtgcgcgt accaagaatc tgtagatccg
     6061 gattttcaat tcattccggg accgtggtat ttctacacaa ttcaaaacgg aaaatgtagc
     6121 tacggatatc aagccgcata cgcattgtta gccgctccgt tcttcaagat ccaccctctt
     6181 ggattcttgt ttctgcactt ctttctctac ctggcaatga tcctgggctt tatgctttgt
     6241 gcgcacaggt tactgccgga aagatcctgg gctccatggt gggcgggaat cattgcggcg
     6301 tggatcatcc cgacggttgt gttcgccatg gatctttcag aagtcattct ttccctggcc
     6361 atgggtgcgt gggccctggc tctcctgtgc aaacgcgaat cctttcagtc tatagaatgg
     6421 aaattacctt cattttcaga attcaaatat tttaattccg ttatcttcgc gtccgggatt
     6481 gtgctgggtc taattctttc gcttcgcacg gaaagtctca tccacggaat ttgctgggcc
     6541 ttcggcttgc ttttggttca gagaaagagt tttgtttcct tcgcgtcagg atacgcagtg
     6601 gccattcttg cggtgatggg atttcatctc tggttctttg ggaatctaat gggcaatcga
     6661 ggaagttctc acgcggctgt gactgcgggg ctcaccgcca gcacgcacat tggaatcgct
     6721 cgaacgttat tgctcggagg cccgctcgga ctaattacaa gcctgcctgc catatttctc
     6781 ggactgtttg ccttttccag gcatgcgcga aagatccacg gacctcaaat tctttttatg
     6841 gcagtcattt ccattttgcc aactctcgga gtgctgatca ctgcaccaag cgacggagga
     6901 tattcctggg gtcctcgctt cctgtcgctt acttttgcgc cctacttcct cctggtcctt
     6961 ctggctctgg atcatcctta cgcgcataga tggcctgcaa tgatcgtcct ggctgcaata
     7021 gcagcgtatg gaatgcaata tacctggaaa ggcaggaatg tgtacagacg cgccatggtt
     7081 caaaatgcag gatacgcggc ctacattgaa tctacttcct cggaagcaat cattggagca
     7141 gactactccc ttctagccat tttgtcgagg tccgcattgc aggggcatgt ataccaggcg
     7201 cgcagcattc ccgaaatcac gattctggcg gatagactca agaacgcagg agtcagaaga
     7261 gtgattcttc tccgttatgc gacatctgaa gtgactcaac tgcctggatg gacagtgaaa
     7321 tcgcgcaaat caatggacct ggttgagttt gttgtttgcg aacgctgagc ggcgacacca
     7381 cgcaacgtga ccgggcgctg tgctctgctg gaagcatcta tcatctttga actgacagcg
     7441 atcatagaat cgacatggag aatggagact gtcatattca gtggcaggag gaacactccc
     7501 gggaatcggc acgagctcac cattgcgcgc acgggcaggc agagactgaa agagtgcctg
     7561 agtgtacgga tgacagggtt ctgaaaatac atcccgaaca gatccgcttt ccaccacctg
     7621 gcctgcgtac aagacgctca tcgtatgtgc ggtctgcgcg acgactccaa gatcgtgcgt
     7681 aatcaaaaga attgcggtgc ccatttcttt ttgaagagca ttcatcaact caagaatttg
     7741 agcctgcaca gtaacatcga gagctgtagt gggctcatcc gc
//
LOCUS       DAOQJL010000126         7725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5155_length_7725_cov_4.491525, whole genome shotgun sequence.
ACCESSION   DAOQJL010000126 DAOQJL010000000
VERSION     DAOQJL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5155_length_7725_cov_4.491525"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLG78_07395"
     CDS             complement(<1..197)
                     /locus_tag="PLG78_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22974.1"
                     /translation="MGSSDHLISNGAVRTGIFSEPVTVVDPAAYDLRSPLGKKLGRRA
                     RHFGYKQFHYVGGISNDLIFGA"
     gene            283..867
                     /gene="coaE"
                     /locus_tag="PLG78_07400"
     CDS             283..867
                     /gene="coaE"
                     /locus_tag="PLG78_07400"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNE22975.1"
                     /translation="MVYKIGLTGAIGSGKSTAARIFAKLGATVLDADVLAKEALEDPD
                     VQTDLKVRFPAAFENGALSHKALADIVFSDRSRLAKLTELTHPVVRRKFKEIVSSMKE
                     GVLVYDVPLLFEAGLEGDFDLTVVISTPDEIRHDRLKKRGLTEEDIKRREGLQLKPAE
                     KENRADVVLTNAGAPEDLENAILKILESSRRALK"
     gene            864..1784
                     /locus_tag="PLG78_07405"
     CDS             864..1784
                     /locus_tag="PLG78_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE22976.1"
                     /translation="MKKRIFYVINLDRGRILVLSVLVTGFMLISFATGYRFGTATEPK
                     RDLGARDEVFMDPAKQGLDRSIAPADSSLSAPLDTSKSEVPRSEKRNESADRTAPDRT
                     SADRSDRNASRPEKETKPRPTQVTENRPEKPAHSNAKTEKPGHAKRETARRPEDKKEN
                     KNEKKNSEKQSRVADRARTEKPASVKPARESKEQRDRPAKNDAKTHNQSKPEKDKSSA
                     VLEPERSMRLADTAPQANKRSFQLGSFSSKDAAGRMTEQLKKQGFAPAIVQSNGKYMV
                     RVGGSGQEDLATLEKQLRAKNYSPIRVSSP"
     gene            1909..2487
                     /locus_tag="PLG78_07410"
     CDS             1909..2487
                     /locus_tag="PLG78_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22977.1"
                     /translation="MYSDWISQKGFDGIPDFFRNHLYSPANKEARDAALDNLYNKLKS
                     VTGPEMTANIHMLIELNRLTDDLDLDTARVLMATTLQTTRPEDLTFDQLFHAVRQTGR
                     KEERKKQVQMVSDALTFFFSLSKLPLIKLVMAPIKVAASLVGAMDLISTMEAGYALSR
                     NIKDMTPFVEAFVKREVEFIDSDYTAHSLIQK"
     gene            2523..4709
                     /gene="ppk1"
                     /locus_tag="PLG78_07415"
     CDS             2523..4709
                     /gene="ppk1"
                     /locus_tag="PLG78_07415"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNE22978.1"
                     /translation="MTQTPYFNREISWLQFNFRVLEEASRDDNPLLERLKFLAISESN
                     LNEFFMVRVAGLKQVVASAVNEIQMDGKTAQETLTEIHRQVSVMVRLQYQYMGDIFAG
                     LKPSGIEFVEDFRSFTEADKEFARQFFENNVFRILTPLAIDPSHPFPLIGNNKLNMAV
                     CLKRPGFEQDFFAIVEVPTVIKRFPELPPGDGGVRRFTALEEIIKLHAPDFFPGTQVT
                     SIQGFTITRNSDIAIDEVASDNLLSTIEDELKQRRWGEAVRLSTRIGMPHHVREFLRT
                     KLDLSPEECFERPGLLNLQDLFEIANALQDRADLANNPFIPRNPVQQKKMSHIFQTIR
                     RKDILLHHPYDSFQPVLDLLDFAAMDPKTLAIKQTLYRTGGDSPLIESLIQAAENGKQ
                     VTVLMELKARFDEERNIVRAREMEQHGVHVVYGLVGLKIHGKMLQIIRREDDGVKAYC
                     HLSTGNYNPNTARGYTDLALMTADREINNDVTNLFHALTGYASMPKFSRIAAAPINLR
                     ETLLSLIQQEIENAEKGMASRIRLKMNALVDPDMIALLYRAARAGVRIELNIRGICCL
                     VPNAEGTEDRIRVISIVGRFLEHSRIFHFENGGDPRVFLASADWMPRNLNRRVEVMFP
                     ILDPDHKHTILEILDIIFKDDHNARLLGPDGTWKRLRPEGGGEGFSSQRYFRDQARKN
                     FEQKEKERESQIVFQPAGSPNTPVLSRLIPVDHEAGERASQSDSGN"
     gene            complement(4744..5766)
                     /locus_tag="PLG78_07420"
     CDS             complement(4744..5766)
                     /locus_tag="PLG78_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNE22979.1"
                     /translation="MIRLTLLIALTLPVLNCATMTIHTGTGNLYGREGQKEEGNYFEP
                     ATKRDASSQMPACKTEPPVAGNDPHNTAVAPEKTPEKTPEKVSDRSAPGYDDKAKDEE
                     ASYFNNQAENPPVVADNSTGEESGTASWYGRDFDGKATASGEPFDSRKLTAAHRTLPL
                     GTVILVKNTENNREALVTVNDRGPFVKGRIVDLSEYGAEMLGYKEKGLTTVSIRVVRR
                     GAMTEKGPGVTSAAFNDADHAQGNGPESVKPQVVTRPKVQAPANIREEKALGKYSVQV
                     GLFSEYDNADKLGKYLTSYNQPVNIFQRGTSYVVKVGEFADKNQAEQLKTRLSSDGYT
                     AFISAP"
     gene            5765..6439
                     /locus_tag="PLG78_07425"
     CDS             5765..6439
                     /locus_tag="PLG78_07425"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HNE22980.1"
                     /translation="MVLSTQGLISSIDRLADHKEDFLRPKPKSRACQAAMRCEKCHLL
                     PSNCICAQIPSIRTRMRLTLILHALEARRSTNTGHLAALALSNSRVEIHGRAGQAIND
                     SFEVTAGMKPLFLFPDGSEELRPEMANDGDVELIVPDASWAQAVRMSRRIHGLSGVPH
                     VRLPRVTPRKILRKSLEGRISTFEAISHAVQILEGEEAGASMLTLFDAYASALLRERG
                     RRSRFD"
     gene            complement(6440..7483)
                     /gene="hemH"
                     /locus_tag="PLG78_07430"
     CDS             complement(6440..7483)
                     /gene="hemH"
                     /locus_tag="PLG78_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNE22981.1"
                     /translation="MARTSDRTGILIINLGTPDSPSTHHVRRYLREFLSDPRVIDIPG
                     LLRFFLLNFIILPLRPARSAEAYRKIWTNEGSPLLIHTRDLSLALARHLPRVSVKFAM
                     RYGSPSIPDVMKEFAKEGVRKIKVLPLYPQYAAASTASTSARVYECAASMNDPLEVEM
                     LGPFFDHDGFLDATAHLARPFLASAKGAHVLFSYHGVPERQIDRSVRSGLSHADQEHC
                     SFGDCCHSPGDRMALCYRAQCFSTTKGLVSRLQLKQEDYSIGFQSRLGRTPWVRPYTD
                     ELIQTLPARGVKHLAVICPSFVSDCLETLEEIQIRAKEDFLRAGGESFVYIPCLNSDE
                     TWVKAVARMVEGE"
     gene            7537..>7725
                     /locus_tag="PLG78_07435"
     CDS             7537..>7725
                     /locus_tag="PLG78_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE22982.1"
                     /translation="MSDELLKQIETKTSDLLKQVTETLAKRSEEKGQVSVSKMDENQL
                     VHYNLSYSTAEKRISDHFT"
BASE COUNT         2078 a         2022 c         1902 g         1723 t
ORIGIN      
        1 gcaccaaaga tcagatcgtt tgaaatcccg cccacatagt gaaattgctt gtagccaaaa
       61 tgcctggcac gccggccaag cttctttccc agcggactcc tcagatcgta tgcagccgga
      121 tccacgacag taactggctc tgaaaaaatg ccggttcgca ccgcaccgtt agatatcaaa
      181 tgatcgcttg agcccattta tctgttttgc gccaatgcgc ctgatgtaaa gccagatcga
      241 aaaattactt gtacttctta aagacccgcg aaacgatgcg ccgtggtcta caagatcgga
      301 ctgactggcg ccatcgggtc tgggaaatca acagccgcac gcatcttcgc aaaactggga
      361 gctacagtcc ttgacgcaga cgtactggca aaagaagcac tggaggatcc ggacgttcaa
      421 acagatctca aagttcgctt ccccgctgct ttcgaaaacg gagccctgtc tcacaaagca
      481 ctcgcggaca ttgtgttctc cgatcgctcg aggctcgcga agcttacgga actgactcat
      541 ccagtggttc ggcgcaagtt caaagaaatc gtttcatcca tgaaagaagg cgttctggtc
      601 tatgatgtgc ctctgctgtt tgaggcgggc ctggaagggg acttcgacct cactgtggtc
      661 atcagcaccc cggatgaaat tcgccacgat cgcctcaaaa agcgcggttt gacggaagaa
      721 gacataaaaa gaagagaagg cctccagctc aaaccggccg aaaaagaaaa cagggcagat
      781 gtagtgctca cgaatgcggg agcaccagaa gacctggaaa acgcaatatt gaaaattctg
      841 gaaagcagcc ggagggctct gaaatgaaga agagaatatt ctacgtaatc aacctggatc
      901 gcggaagaat cctggtactc tctgtactgg tcaccggatt catgttgatc tcgtttgcaa
      961 caggctacag attcggcacg gctacagagc caaagcgcga tctgggagcg cgcgacgaag
     1021 tattcatgga cccggccaaa caagggttag acagatccat cgccccggca gactccagcc
     1081 tcagcgcgcc cctggacacg tccaaatccg aagttcccag atcggaaaag agaaatgaat
     1141 ctgcggatcg cacagctccc gatcgcacct ctgcagatcg cagtgatcgc aatgcatcgc
     1201 ggcccgaaaa agaaaccaaa cctcgcccaa ctcaggtaac ggaaaatcgc cctgaaaaac
     1261 cggcgcattc aaacgcaaaa acagaaaagc caggccacgc gaagcgcgaa acagcccgtc
     1321 gcccggaaga caaaaaagaa aataagaacg aaaaaaagaa ttctgagaag caatctcgcg
     1381 tagcagatcg ggccagaaca gaaaaaccag cgtctgtaaa gccggcgcgc gaaagcaaag
     1441 aacagcgcga tcgcccggca aaaaatgatg cgaagactca caaccagtcg aagccagaaa
     1501 aagacaaatc gtccgctgta cttgagccgg aacggagcat gagactcgca gacaccgctc
     1561 cgcaggcaaa taaaaggtct tttcagctgg gtagcttctc ttctaaagat gcggccggcc
     1621 gcatgacaga gcagctcaag aagcagggtt ttgcgcccgc catcgtccag tcgaacggca
     1681 aatacatggt gcgagtgggt ggttccggac aggaagacct ggcaaccctg gaaaagcagc
     1741 ttcgcgcgaa gaactacagc ccgattcgcg tctcctcacc ctgagggttt tagacttgtc
     1801 accaaaaggc cttttgggcg ctcttatcct gtgaccgcac aattgacgga tgaagaaaag
     1861 tccatccgac tgctggtagt aagccggacg attgatcgcc tggctgtcat gtattccgat
     1921 tggatttccc agaagggatt cgacggcatc cctgacttct tccgcaacca tctctattca
     1981 cctgcaaaca aagaagcgcg ggacgcagcg ctggataatc tctacaacaa gctcaagtca
     2041 gtcacgggtc cagaaatgac cgccaacatc catatgctca ttgagctcaa tcgcctcaca
     2101 gacgatctgg atctggacac ggcccgggtt ctcatggcca ccactcttca gaccaccaga
     2161 ccggaagatc tcacgtttga tcaactcttc cacgccgttc gccagaccgg cagaaaggaa
     2221 gagagaaaga aacaggtcca gatggtatca gacgccctga ctttcttctt ctccctttcc
     2281 aaattgcctt tgatcaagct ggtgatggcg cctatcaaag tagcggcatc cctggtgggc
     2341 gccatggatc tgatttctac aatggaagcg ggctacgcac tttcccgtaa cataaaagac
     2401 atgaccccgt tcgtagaagc atttgtgaaa cgcgaagtgg aattcatcga ttccgactat
     2461 acagcccatt cgctgataca aaagtaatag tctgggctca ctgccctctc agaacataga
     2521 ctatgacgca aacgccttac ttcaaccgcg agatatcctg gcttcaattc aacttccgcg
     2581 tgctggaaga agcgtcccgc gatgacaatc ctctcctgga aagactgaag ttcctggcca
     2641 tctcagaatc caatctaaac gagttcttca tggtgcgagt ggcaggcctc aagcaggtcg
     2701 ttgccagtgc ggtcaacgaa atccagatgg atggcaagac cgcgcaagaa actctcacag
     2761 agattcatcg ccaggtgtcc gtgatggttc gcctgcagta tcaatacatg ggcgatatat
     2821 tcgcaggtct caagccatct ggaattgaat tcgtagaaga ctttcgctca tttacagaag
     2881 cagacaaaga atttgcgcgc cagtttttcg aaaacaatgt gtttcgtatt ctcactcctc
     2941 ttgcgatcga tccttcgcat ccgtttccac tgattggaaa taacaaactc aacatggcag
     3001 tctgcctgaa gaggccagga tttgagcagg atttctttgc catcgtagaa gtgcccacag
     3061 tcatcaaaag atttcctgaa cttcctccag gggacggagg cgtgcgccga ttcactgcac
     3121 tggaagaaat catcaaactc cacgctccag acttctttcc tgggactcaa gttacaagca
     3181 ttcagggttt taccatcact agaaactcag acatagcgat cgatgaagtg gccagcgaca
     3241 atctactgtc cacaattgaa gatgaactca agcagagaag atggggagaa gcagtccgat
     3301 tgtctacgcg cattggaatg cctcatcacg tgcgggaatt tttgcgcacg aagctggatc
     3361 tttcgccgga agaatgtttc gaaaggcctg gactactcaa tcttcaggat cttttcgaaa
     3421 tcgcaaatgc tctccaggat cgtgcagatc tggcaaacaa tccgttcatt ccgcgcaacc
     3481 ccgtgcagca aaagaagatg agtcacatct tccagaccat tcgcagaaaa gacattctac
     3541 tccatcaccc gtacgattca tttcaaccgg ttctcgattt gctcgatttt gcggccatgg
     3601 atccaaagac tctggccatc aagcagacat tgtatcgcac gggtggagac agtccactca
     3661 ttgaaagcct catccaggca gcagagaatg gcaaacaggt gactgttctc atggaactca
     3721 aagcacgatt cgatgaagaa agaaacatcg tgcgcgcgcg cgaaatggaa cagcatggcg
     3781 tccatgtagt ctacggactc gtaggtctca agattcacgg aaaaatgctg caaatcattc
     3841 gaagagaaga tgacggagta aaagcgtact gccatctttc tactggcaat tacaacccga
     3901 acactgccag aggttataca gacctggcac tgatgactgc ggatcgcgaa atcaacaacg
     3961 acgtaaccaa cctctttcac gcgctgaccg gttacgcgtc catgccaaag ttttcaagaa
     4021 tagcggccgc ccccatcaac ctgcgcgaaa cgcttctatc tctgatccag caagaaatcg
     4081 aaaacgctga aaagggaatg gcatcgcgca ttcgcctgaa gatgaacgcg cttgtagatc
     4141 ctgacatgat cgcacttctc tatcgtgctg caagagcagg agttcgcatt gaactgaaca
     4201 tcaggggcat ttgttgcctt gttcccaatg cggagggaac agaagataga atcagagtga
     4261 tctccattgt tggacgtttt cttgagcatt cgcgaatctt tcattttgaa aatggcggag
     4321 atccgcgcgt cttccttgcg tctgcagact ggatgccgcg caatctgaat cgccgcgtgg
     4381 aagtgatgtt tccgattctg gatccagatc acaaacatac gatcctggaa atcctggata
     4441 tcatcttcaa agatgatcac aacgccagac tacttggacc ggatggcaca tggaagagac
     4501 tcaggccaga aggtggcgga gaaggtttct cttcgcaaag gtactttcgc gaccaggcaa
     4561 gaaagaattt tgagcaaaag gaaaaggaac gcgagagcca gattgttttt cagccagcag
     4621 gaagcccgaa cacgcccgtt ctttccaggc tcattccagt ggatcacgaa gcaggggaaa
     4681 gagcatccca gagcgattca gggaactgaa cacgaggggc gcggagcagg gcgaaggcgc
     4741 gccttacggc gcgctgatga atgcagtgta gccgtcagat gacagcctgg ttttgagttg
     4801 ttctgcctgg tttttatccg cgaactcccc taccttcaca acgtagcttg tgcctctctg
     4861 aaaaatattc accggctggt tgtagctggt aagatattta ccaagcttgt ctgcattgtc
     4921 atactctgaa aagagtccaa cctgcactga atacttacca agcgcttttt cttcgcggat
     4981 gttcgcaggt gcctgaacct tagggcgcgt tacaacctgt ggtttgacgc tttccggtcc
     5041 gtttccttgc gcatgatctg catcattgaa tgcggcagac gtaacgcctg gacctttctc
     5101 agtcatggct ccacgacgca ccactcgaat cgaaacagtg gtcagtcctt tctctttgta
     5161 accgagcatt tctgcaccat attcgctaag atccacaatg cgccctttta cgaaaggtcc
     5221 gcgatcgttt acagtcacga gtgcttcgcg attgttttca gtgttcttta caagaatgac
     5281 tgtgcccaga ggcaaagtgc gatgagcggc ggtgagcttg cgcgaatcaa atggttcacc
     5341 acttgccgtt gctttgccgt caaagtcacg accataccag gatgcagttc ctgactcttc
     5401 tccagtagaa ttgtctgcca caaccggtgg attctctgct tgattgttga agtaactagc
     5461 ttcttcatcc ttagccttgt cgtcataccc tggcgcagaa cgatcactta ccttctcagg
     5521 tgttttttcc ggagtcttct ctggtgccac agcagtattg tgcggatcgt ttccagctac
     5581 aggtggttct gtcttgcatg caggcatttg cgaggatgca tcccgtttgg ttgcaggctc
     5641 gaaataattg ccctcttctt tctggccttc gcgaccgtac agatttcctg ttccggtatg
     5701 aatggtcatt gtggcgcaat tcaatactgg aagcgtcagt gcaattagta gggtaagacg
     5761 gatcatggtt ctctccacac agggactcat ttccagtatc gaccgactcg cagaccacaa
     5821 agaagatttc ttgcgtccaa aaccaaaatc gcgagcctgc caggctgcca tgagatgcga
     5881 gaaatgccac ctcctcccgt ctaattgcat ctgcgcgcag attccctcta ttcgcactcg
     5941 aatgcggctc accttgatcc tgcatgccct ggaagccaga cgttccacga acacggggca
     6001 tctggcagca ctggcacttt ccaattcgcg cgtagaaatt catggccgcg caggccaggc
     6061 aatcaatgat tcttttgagg tcactgccgg aatgaagcct ctgtttcttt ttccggatgg
     6121 atcagaagaa ttgcggcctg aaatggcgaa tgacggagat gtggagctca ttgttccaga
     6181 tgcaagctgg gctcaggcag ttcgaatgag cagacgcatt cacggactct ctggagtgcc
     6241 acacgtacga cttccgcgag tcacccccag aaagattttg agaaagtctc tggagggacg
     6301 catttccacg tttgaagcga tttcacatgc agttcaaatt ctagagggcg aagaagcagg
     6361 cgcatccatg ctcactctat ttgacgcata cgcgtccgct ctgctcaggg agcgcggaag
     6421 aagaagcaga ttcgactgac tattctccct caaccattct ggccactgcc ttcacccagg
     6481 tttcatccga gttcaggcac gggatataca caaacgattc tccacccgcg cgcaagaaat
     6541 cttcttttgc gcgaatttga atttcttcca gagtctcaag acaatcagaa acaaacgaag
     6601 gacaaatcac cgccagatgt ttgactccgc gcgctggaag cgtctggatc aattcatctg
     6661 tgtagggtcg aacccacgga gttcttccga ggcgagattg aaaaccaatg ctgtagtctt
     6721 cctgcttcag ctgcagcctg gacacgagtc ccttcgtcgt gctaaaacat tgagcgcgat
     6781 agcacagtgc catgcgatct ccaggagagt gacaacaatc tccgaaggaa caatgctctt
     6841 gatctgcgtg gcttaaacct gaacggacag aacgatcaat ttgtctttct ggcactccgt
     6901 ggtagctgaa tagaacatgg gctcccttcg cactggccag aaagggacga gccaggtgtg
     6961 ccgttgcatc cagaaatcca tcatgatcaa aaaagggccc gagcatttct acttctaaag
     7021 gatcgttcat ggaggcggca cattcgtata cgcgcgcgga tgtggaagca gtagaagcgg
     7081 ccgcgtactg cggatagaga ggaagcactt tgatcttacg tacgccttcc ttcgcgaatt
     7141 ctttcatgac atccggaatg gaagggctac cgtatcgcat ggcgaacttc acgcttacgc
     7201 ggggcaaatg acgcgcaaga gcaagagaca ggtcgcgagt gtgaatcaaa agaggagaac
     7261 cttcgttcgt ccagatcttc ctgtaagcct ccgcagaacg agcgggcctg aggggcagta
     7321 tgatgaagtt taatagaaag aatcgcaaca gtccagggat gtctatgact ctgggatcgc
     7381 ttaaaaactc gcgcagatac cgacgaacat ggtgcgtgga ggggctatcc ggagtgccga
     7441 gattgataat cagaatgcca gttctgtctg aggttcgggc catagtctta ccttaatttg
     7501 attggccgcc aaaattcaaa atcaaaaagt gggtctatgt ctgacgaatt gctgaaacaa
     7561 attgaaacca aaacaagcga ccttctaaaa caggtaacgg aaactctcgc aaagagatcc
     7621 gaagaaaaag gtcaggtatc cgtctctaag atggatgaga accagctggt tcactacaat
     7681 ctctcctact cgactgctga aaaaagaatc tccgatcatt tcacg
//
LOCUS       DAOQJL010000127         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5165_length_7711_cov_4.284091, whole genome shotgun sequence.
ACCESSION   DAOQJL010000127 DAOQJL010000000
VERSION     DAOQJL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5165_length_7711_cov_4.284091"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLG78_07440"
     CDS             complement(<1..568)
                     /locus_tag="PLG78_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNE22983.1"
                     /translation="MKGLLTLDQLKEKHAKGEIETVLVAFPDQYGRFMGKRYTASFFL
                     DAVARDGMHACDYILTVDMEMDVIPGYKLSSWEKGYGDFHCKPDMSTLRQLTWLDRTA
                     LVICDLVNEKTHALVGPSPRAMVQHQIDRLRAAGYAGLGASELEYFMFDETYESAAAK
                     NYENLKTFGNYIEDYHIFQGTKAERLNAA"
     gene            complement(571..1227)
                     /locus_tag="PLG78_07445"
     CDS             complement(571..1227)
                     /locus_tag="PLG78_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE22984.1"
                     /translation="MMQTLHNLVIVWFGLVHDWGYGGIVFLMALESSIVPIPSEVVMP
                     PAAFWAAQGKMNFWFVVLAGTVGSYLGSVISYFVARSIGSPLLYRFGKFIFLPPEKLK
                     LAEAWFNTYGAAGIFFARLLPVIRHLISIPAGVFKMNFAAFSTWTIVGAGLWCIVLSW
                     YGGQVLGESPQLLESPIEMVEVLKAKMYWIIAGVAGIAILYGIMVFAKRRLLEKKPEA
                     "
     gene            1343..2155
                     /locus_tag="PLG78_07450"
     CDS             1343..2155
                     /locus_tag="PLG78_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE22985.1"
                     /translation="MGGKLNTLRVVLVESRATFLRAFRHTREDWRTLVRATGIFSACL
                     AVVYTIGVFAVMRFTYSLAFTAPEYIRELQNILLNVIAMPFLAYFLGGLLQFFRERTR
                     GTQAPYILLIKGHRHYFRILLYCLAYYAGYRAFVRTVVGIEDYPVDLRLRMLFGLPLF
                     IWVITRVCFSFAFVTEQDAGPKLGIKSSFILTGGRFWRTLVILFPVPVSFATAGLCIG
                     LLPHPVLVILALILVCAALPFVLSVVFLSYFWLFDRYRQEPTVVEIAAVTGQ"
     gene            complement(2164..3555)
                     /locus_tag="PLG78_07455"
     CDS             complement(2164..3555)
                     /locus_tag="PLG78_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE22986.1"
                     /translation="MRAVIFFCLCLMSCIDVYENVSSVDISDIKKRGKLLALTTYGPN
                     SYFLFKGQPLGYEYELLSRLADDLGVKLELVIVKDVETMPYMLNSGAGDIVAANIPVT
                     RKKAMTIQFTEHLTLTRQVLVQRAQGYKPAGRPDLKEGRPIRNTVDLIGQKVHVREGS
                     PYATRLKSLSEEIGGEISIVTVPGDVTTEDLIKKVSEGEFDYTVSDESTALINQAYYP
                     GIDVETPISFPQRIAWVVRNKSPELLSTVNAWIKRMKTTDSYGVIYNRYYRDPKQYRD
                     RLASQFFTVSGGRISPWDQTFRESAKPMGMDWRLLAAIAYQESRFDPTARSWAGAQGV
                     MQLMQPTAVQFGAPNAFVAEANIKAGAKYLAWLDEQWKSITDPDERIKFVLASYNAGP
                     AHVEDARRLATRYKKDPNKWFDHVEIFILDLSRPEFYNDPVVQYGYLRGEEPYHYVRE
                     ILERYEHYKKFVK"
     gene            3658..4842
                     /locus_tag="PLG78_07460"
     CDS             3658..4842
                     /locus_tag="PLG78_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE22987.1"
                     /translation="MQFPIDRVVQYNFPTRIRSGFGARHEIGPYLLAEGQKRPLIVTD
                     AELAKLPIPGDLEKELNGAGLSAKIFAGVQGNPVESQVTAGVAVFRAHNADSIVALGG
                     GAAMDVAKAITLMAHHPGKLFDYEDVPGTKPVDQTIPFFVTVPTTAGTGTEVGRSTVI
                     SDDTTHAKKIMFSPRLLARCVFADPELTLGLPARVTAATGMDALTHLVEAYLANDYHP
                     ICDGIALEGVRLVAQSLVQCTEYAKAKDPGSDDHRRVRGMMLNAAMMGGIAFQKGLGA
                     VHSTAHPLSTVFDFHHGLANGIMIPYVMRFNAEAVPERFVTLAQAAGIANPGPDSFIH
                     WLENLKEQLGIPRTISEAGAKKEGLEDLVRFAVLDVCHAMNPRPVSEGDFRNIYTKAF
                     GS"
     gene            4839..5585
                     /locus_tag="PLG78_07465"
     CDS             4839..5585
                     /locus_tag="PLG78_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNE22988.1"
                     /translation="MSLRIGVSACFFHADPKRPIFKGKTLVYAEASMLQWVMSGGAIP
                     VMIPPPYAGFAIKDFLAEVDGVLLQGGSDVSPTSYGETPLKPEWNGDRVRDDYEKSII
                     ETAIEMDRPVLGICRGLQIMNVALGGSLYQDIREQNSASIVHRDWDIYDDLLHRVRID
                     PSSRLGAMYGEGGMINSIHHQGIKDLSPQLSAEAWCVEDGIIEAVRLKSNKAYCLGIQ
                     WHPEFIRKDQNLLDPAPILKEFLTEVSNRK"
     gene            5582..7048
                     /locus_tag="PLG78_07470"
     CDS             5582..7048
                     /locus_tag="PLG78_07470"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE22989.1"
                     /translation="MIDLARDFARLTEKLKNSKEISIPLQSLESEEEIFANGLVTKAL
                     AYIGQIRLEGTLHLAVAELLSNAEKALLKRTFAEFFANTADYDNPDFLKSFHRKLRED
                     LRSLRAILKKSKPSIEVNMKIQASGLSLSILTGSMPSLQEDEFIHRAMEAGLRAKSLA
                     DLEALPDTIEAEGRGLAISVFSLKSAGIPGIVYALQGDRACFSISVPAHRPDENFLSR
                     IGSDLIGEIQSLPAFPDTTRKLLEMCNSPDTDLKSIALEIGRDAAIAGELIRVANSGG
                     FAGGRVADIAEAAKILGMSNITGLLMRSAAYAILDERYGVTEELLAHPVRVAVYSRTL
                     ARKYKLSAIADQAYVAGLLHDIGKIVLVHHMKQTGSLSSVEKTSRFRDRRSQVNLEEI
                     ACGADHALIGKLLGEKWNFPDELCMAIGMHHSPLGAPKETRKLAFVVYMANALADYEE
                     GQTEFYSVEPEVLAFFNIATVQAFQMLAGSLNSEYSST"
     gene            complement(7091..>7711)
                     /locus_tag="PLG78_07475"
     CDS             complement(7091..>7711)
                     /locus_tag="PLG78_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE22990.1"
                     /translation="RQQEYEKWLSDYQDEDREAVEIFRNRWEITPTALGILFFLIAAA
                     FFIIRFRFPSWLSGSGIDAHAQIALTVVLPAFAAAVLLLRDKINYLYFRETMLRVPSG
                     TLVHIEHERGTSQDTMIFDILPGGAFLSGVDDLSQGDRLHLSISRNNKPSLRVRCAVR
                     WVNNVNRQLPTGALVQFLKPEPGFLLFMMIHALKRIHLRPVTRHAG"
BASE COUNT         1811 a         1965 c         1958 g         1977 t
ORIGIN      
        1 tggcagcatt caatctctcc gctttggttc cctggaaaat gtggtaatct tctatgtaat
       61 tgccaaatgt tttgagattt tcgtaatttt ttgcggccgc actttcatac gtttcatcaa
      121 acatgaagta ttccagttcg cttgcaccga ggcccgcata tccagccgcg cgaagtctgt
      181 ctatttgatg ctgcaccatg gccctgggcg atggacctac aagcgcatga gtcttttcat
      241 tcaccagatc gcaaatcaca agtgctgtgc gatccagcca cgtaagttgg cgaagagtgc
      301 tcatatctgg cttgcagtgg aagtctccat accctttttc ccagctcgac aatttgtatc
      361 ctgggattac gtccatttcc atgtctacgg tcagaatgta atcgcaagca tgcattccat
      421 cacgagccac tgcatccagg aagaacgatg ccgtataacg ttttcccatg aagcgaccgt
      481 actggtcagg gaaagcgacc agaaccgttt cgatttcgcc ttttgcgtgt ttttctttca
      541 gctggtctag agtgagcaat cccttcatag ttatgcctcc ggctttttct cgagcaaacg
      601 tctcttcgca aacaccatga tgccgtacag aattgcaatc cctgcaactc cggcaatgat
      661 ccagtacatc ttcgctttga ggacttctac catttctatg ggtgattcga ggagctgtgg
      721 actttccccg agaacctggc caccgtacca gctcagcacg atgcaccaca ggccggcacc
      781 cacaatggtc caggtggaga acgcggcgaa gttcattttg aatacgcccg ccgggataga
      841 aatcaaatgc cgaataacgg gaagcaagcg tgcgaagaag atgccagccg ctccgtatgt
      901 attgaaccat gcttccgcaa gtttcagttt ttcaggtggc agaaagataa attttccaaa
      961 acgatagaga agcggacttc caatggaccg ggcaacaaag taactgataa cacttcccag
     1021 gtagcttccg actgttccgg cgagcacaac gaaccagaaa ttcatcttac cctgtgcggc
     1081 ccagaaggcg gcgggtggca taacgacctc agaaggaata ggcacgatag agctttccag
     1141 ggccatcagg aagacgattc ctccgtagcc ccagtcatgc accagaccga accagacgat
     1201 gacgagattg tgcagagttt gcatcataga gttcgtgcat ttcgcattct gccgtaacca
     1261 ctggctagcg aatttttgca gaggagcccg gccgcagtct gaatgatgtt gactctttcc
     1321 agggcccgca gacaattcgg gcatgggggg aaagctcaat actttgcgcg tcgtccttgt
     1381 ggagagtcgc gcaactttcc ttcgtgcgtt tcgtcatacc cgcgaagact ggcggactct
     1441 cgtgcgcgcc acaggaattt tcagtgcctg cctggccgtc gtctatacca tcggtgtgtt
     1501 tgctgtgatg cgattcacct attctcttgc ctttactgct ccagagtaca tccgcgaatt
     1561 gcagaacatt ttgctcaatg tcatcgccat gccttttctt gcctattttc tcggagggct
     1621 actgcagttc tttcgtgagc gaaccagagg cacccaggcg ccttacattt tactcatcaa
     1681 gggtcacagg cattacttta gaatcctatt gtattgcctg gcgtactatg ctggatatcg
     1741 cgcgttcgtt cgcacggtgg ttggaatcga ggattaccct gtggatctca ggctcaggat
     1801 gctgtttgga ttgcctctct tcatctgggt catcactcgc gtgtgtttct cttttgcgtt
     1861 cgtaacagaa caggatgcag gtcccaagct tggaattaag tcttctttta ttttaacggg
     1921 ggggcggttc tggcggactc tcgtgatctt gtttcctgtt cctgtgagtt ttgccaccgc
     1981 cggcttgtgc attggcctct tgcctcatcc ggtacttgtg attctggctt tgattctggt
     2041 gtgcgcggcc ctgcccttcg tgctctctgt cgtatttttg tcctatttct ggctctttga
     2101 tagatacagg caggagccaa cggtcgtgga gattgccgca gtcacaggcc agtaatagtt
     2161 aatttatttt acaaatttct tgtagtgttc gtatctctcc aatatttctc tcacatagtg
     2221 atacggttct tctcctctca gatatccata ctgcaccacc ggatcgttgt aaaactcggg
     2281 gcgtgaaaga tccaggatga agatctccac atggtcaaac cacttgtttg gatccttttt
     2341 gtagcgagtg gccagtcttc tggcgtcttc cacgtgagcg gggcccgcat tgtacgaggc
     2401 caatacaaat ttgatgcgtt cgtccggatc tgtgatggat ttccattgtt catcgagcca
     2461 ggccaggtat ttcgcgcctg ctttgatgtt tgcctctgca acaaaagcat tgggagcccc
     2521 aaattgcact gctgtcggct gcatgagctg catcacaccc tgtgcgcctg cccacgatct
     2581 tgcagtcgga tcgaatctgg attcctggta ggcaatcgcg gcgaggagcc tccagtccat
     2641 tcccattggc tttgcgcttt ctctaaatgt ctggtcccac ggcgaaatgc ggccgccgct
     2701 tactgtgaaa aattgactgg ccaggcgatc tctgtattgc ttgggatctc tatagtatcg
     2761 attgtatata acaccatacg aatctgttgt tttcattctt ttgatccacg cattcacagt
     2821 ggatagaagc tccggcgact tgtttcgaac cacccacgca attctttgcg gaaagctgat
     2881 aggcgtttcg acatctattc ctggatagta agcctggttg atgagtgcag tgctttcgtc
     2941 ggagacagtg taatcaaatt ctccctctga tactttcttg atcaaatctt ccgtagtaac
     3001 atctcccgga accgtgacga tgctgatttc tccgccaatt tcctcagaaa gacttttcag
     3061 tcgcgtagcg tacggagagc cttcgcgcac gtgtactttc tggccaatca aatccactgt
     3121 gtttcgaatc ggtctccctt cctttagatc tggtcttccc gccggtttat acccctgagc
     3181 acgttgcacc agaacctgtc tggtcaacgt taggtgctca gtgaactgga tcgtcattgc
     3241 tttcttgcgc gtcacaggaa tattcgcggc gacaatatct ccggcaccgc tgttgagcat
     3301 atacggcatg gtttctacat ctttgacgat caccagttcc agtttgactc ccagatcatc
     3361 tgcaagacgc gaaaggagtt catactcata tccgagaggc tggcctttaa aaagaaaata
     3421 gctgttgggg ccatacgtgg taagtgccag aagcttgccg cgtttcttga tatcagagat
     3481 atccacagaa gaaacattct catacacatc gatgcaggac ataagacaga ggcaaaaaaa
     3541 gattacggcg cgcatggaag gtccccctca tcatcctgcc agcctggcct cagaacatgc
     3601 aagcgatttg accgcaaaaa aggatgactt ccaaagctga ggctagaatc cgagatcatg
     3661 cagttcccca tagatcgagt tgttcaatac aattttccaa cacgcattcg ctccggtttt
     3721 ggtgcgcgcc atgaaatcgg cccgtatctg ctcgcggagg gccagaaacg cccgctcatc
     3781 gtaacggatg cagagcttgc gaagctgccg atcccagggg atctggaaaa agaactcaat
     3841 ggcgcggggt tgtctgcgaa gatcttcgca ggcgtccagg gcaatccggt ggagtctcag
     3901 gtaacggcag gagtcgcggt ctttcgcgcg cacaacgcag actccattgt agctttaggc
     3961 ggcggagctg ccatggatgt tgccaaagca atcacactca tggcgcatca cccgggcaaa
     4021 ctctttgact atgaagatgt tcctggaaca aagccagtgg atcagacgat tcctttcttc
     4081 gtcacggttc caaccaccgc tggcacagga acagaagtgg gtcgcagtac tgtgatctct
     4141 gatgacacaa cgcatgcgaa gaagatcatg ttttcgccgc gactgctcgc tcgttgtgtt
     4201 ttcgcggatc cggaactgac gctcggtctg cctgctcgtg taacagccgc tacagggatg
     4261 gatgcgctca ctcatctggt agaagcgtat cttgccaatg attatcatcc aatctgcgac
     4321 ggcatcgctc tggaaggagt gaggctggtg gcgcaaagtc tggtgcagtg tacggaatat
     4381 gcgaaagcaa aagacccagg ctcggatgat cacagacgcg tacgcggcat gatgctaaac
     4441 gccgctatga tgggtggaat cgcttttcaa aaggggctcg gtgccgttca ttcaacggct
     4501 catcctcttt ccacggtctt tgattttcat cacggtcttg caaacggaat catgattcct
     4561 tacgttatgc gctttaacgc ggaggcagtg ccagagcgat ttgtcactct ggcccaggca
     4621 gcgggaatcg ccaatccagg ccccgactct ttcattcact ggcttgaaaa tctaaaagag
     4681 caacttggca ttccacgtac catttctgag gccggtgcga agaaagaagg tctcgaagat
     4741 ctggtgcgat ttgcagttct cgatgtctgt catgcaatga atccgaggcc tgtgagcgaa
     4801 ggcgacttta gaaacatata cacgaaggca tttggatcgt gagtttgcgc attggtgttt
     4861 ccgcctgctt ttttcacgca gacccgaaga gacctatctt taagggcaaa acacttgtat
     4921 atgcggaagc atccatgttg cagtgggtca tgtcaggtgg cgccattccc gtaatgatcc
     4981 ctccacctta cgctggtttt gcgattaaag attttctcgc ggaagtagac ggagttcttc
     5041 ttcagggcgg atcggatgtg agccctacga gttacggcga aactcctctc aagccggaat
     5101 ggaacgggga ccgcgtacgc gatgattatg agaaaagtat cattgaaacg gccattgaga
     5161 tggatcgccc tgtacttggc atttgtcgcg ggctacagat catgaatgta gctctgggtg
     5221 gttcgcttta ccaggatatt cgagagcaaa attcggcttc tattgttcat cgcgactggg
     5281 atatttatga cgatttgctc cacagagtca ggatcgatcc gtcttccagg cttggggcca
     5341 tgtatggcga aggtgggatg atcaattcaa ttcaccatca ggggatcaag gatctctctc
     5401 cccaattgtc tgcggaagcg tggtgcgtgg aagacggcat cattgaagca gtgcgattaa
     5461 agtcgaacaa agcatattgc ctcgggattc aatggcatcc tgagttcatt cgcaaagacc
     5521 agaatttact cgatccggcc cccattctga aggagtttct gacggaagtg agcaatcgaa
     5581 aatgatcgat ctggcccggg atttcgcgcg gcttactgaa aaactcaaga actcaaaaga
     5641 aatcagtata cctttgcagt ctctggaaag tgaagaggaa atctttgcaa acggtctggt
     5701 caccaaagct cttgcataca ttggtcagat tcgtctggag ggtacgcttc atctggcagt
     5761 ggcagaacta ctgagcaacg ccgaaaaagc acttctgaaa agaacattcg cagaattctt
     5821 cgcgaacact gcggactacg acaatcctga tttcctgaaa tcctttcatc gcaagctgcg
     5881 cgaagatttg cgttctctgc gtgccattct taaaaaatct aaaccttcca ttgaagtgaa
     5941 catgaagatc caggcgtccg gtctgtcttt gagcattctc acgggttcta tgccttccct
     6001 gcaggaagat gagttcattc atcgtgccat ggaggctggt ctgcgtgcga agtctctggc
     6061 agatctagaa gcgttgccgg atacgatcga agcggaaggg cgcggacttg ccatatctgt
     6121 tttctctctt aaatctgccg gaatccccgg gatcgtctac gcgctgcaag gcgatcgagc
     6181 ctgtttttcg atttccgtcc ccgcacacag gccagatgag aattttcttt cgcgcatcgg
     6241 ctcagatttg attggcgaaa tccaatctct ccctgcattc cctgatacta cgcgaaaact
     6301 ccttgagatg tgcaactcgc ctgacacgga cttgaaaagc attgccctgg aaattggaag
     6361 agacgcggcg attgccggcg aactcatccg cgtggccaat tcaggtggat tcgcaggagg
     6421 gcgtgtagcg gacattgcgg aggcagcaaa gattctgggc atgtccaaca ttacgggctt
     6481 actcatgcgc tctgccgcct acgccattct ggatgaacgc tacggagtca cagaagagct
     6541 gcttgcacat ccggttcgtg tggcggtgta ttcgcgcaca ctcgctcgca agtacaaact
     6601 gagtgcgatc gcagaccagg cgtacgtggc cggtctcctg cacgacattg ggaagattgt
     6661 gttggtccat catatgaagc agaccggaag cctctcctcc gtagagaaaa cctccaggtt
     6721 cagagatcgg cgaagccagg tgaacctgga ggaaatcgcc tgcggtgcgg atcacgcctt
     6781 gatcggaaag ctcctgggtg aaaaatggaa tttcccggat gaattatgca tggccattgg
     6841 aatgcatcac agtccactcg gggcgccaaa agaaacgcgt aagcttgcat tcgtggtgta
     6901 catggccaac gctctggctg attatgaaga gggacagaca gagttttatt ctgtggaacc
     6961 tgaagtgctc gcgtttttca atatcgcaac agttcaggcc ttccagatgc ttgccggttc
     7021 actgaattca gagtacagca gcacgtgacc tccgtgggcg gcacgtgacc gggcgcaacg
     7081 taatatttca ctagccggcg tgtcgtgtaa cagggcgaag gtggattctc ttcagcgcgt
     7141 ggatcatcat gaacaacaga aatccaggct caggcttcag aaactgaacg agcgcacccg
     7201 tcggcagttg tcgattcaca ttgttcaccc accgaacggc gcaccggacc cgaagggatg
     7261 gtttgttgtt tcttgaaata gataaatgaa gtctgtctcc ctgggaaaga tcgtccaccc
     7321 cggatagaaa agctccaccc ggcagaatat cgaatatcat cgtatcctgg gaagttcctc
     7381 gttcgtgctc gatatgcacc agagtaccgg aaggcactcg aagcattgtt tctcgaaaat
     7441 agaggtagtt gatcttgtcg cgaagcaaca gtacagccgc cgcaaaggca gggagtacaa
     7501 ctgttaaagc aatctgcgcg tgagcatcaa ttccagaacc agagagccac gatggaaagc
     7561 gaaaacggat tataaagaaa gcggctgcga tcagaaaaaa caaaatgccc agagcagtgg
     7621 gcgtgatttc ccagcggttg cgaaagattt ccactgcttc cctgtcttcg tcctgatagt
     7681 cgctcaacca tttttcatat tcttgctgcc t
//
LOCUS       DAOQJL010000128         7683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5193_length_7683_cov_4.665181, whole genome shotgun sequence.
ACCESSION   DAOQJL010000128 DAOQJL010000000
VERSION     DAOQJL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5193_length_7683_cov_4.665181"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="rplO"
                     /locus_tag="PLG78_07480"
     CDS             <1..399
                     /gene="rplO"
                     /locus_tag="PLG78_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE22991.1"
                     /translation="RAGYKKKLGFEGGQNPLRLRIPKRGFTNIFATRYQVVNLKDLTE
                     AGFSGEVNPEALKGKGLISRPGMPVKILGVGTVSGKLSVTADAFSATAKAAIEKSGGT
                     CTIRPDYIPRVDRKFEKKAQRKTRLGKGRA"
     gene            396..1748
                     /gene="secY"
                     /locus_tag="PLG78_07485"
     CDS             396..1748
                     /gene="secY"
                     /locus_tag="PLG78_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNE22992.1"
                     /translation="MNALINIFKIPELRRKILFTAGLVIVYRLGSFITIPGVNPIAVA
                     QSRPPGQSFLDVVNVFSGGALFKLSIFSLGIMPYISASIIMSLLTVVIPQLARMQREG
                     EDGRRKINQYTRYGTVLLCAIQALFILVWAVDQKNPNNGEPFVRMARTWFFVVGLGAI
                     TTGTILLMWMGEQITERGIGNGASLIIFSGIIAQLPSKLAQMMKDKSISPFDIVILVS
                     LFLILIALTILLSQGVRKIPLQYGKKMQGRRLVQARSQSIQFKLNSANVMPIIFASSL
                     LLFPQTVIGMLGEDSRGWIGWRILQDWLNPFAPEFYKQLPYYTIYTILIIFFAYFYTA
                     IYINPQELSENLKKYGGFVPGIRPGTHTKEYVESTLNRIILPGAIFLAGLALAPYLII
                     NFLGFKANANIQGLAYTFGGTSLLIIVGVALDTLKQIESQLIMRNYDGFMKKGRIKGR
                     "
     gene            1753..2322
                     /locus_tag="PLG78_07490"
     CDS             1753..2322
                     /locus_tag="PLG78_07490"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNE22993.1"
                     /translation="MRIVFMGPPGAGKGTQAKIICDRYGIPQVSTGEILRAAVKNGTD
                     MGKKAKEYMDAGKLVPDSVVIGIVKDRIREPDAQKGYILDGFPRTLEQADALKAMLAE
                     IKTPLQIALNLDVRDDLLIARLLDRAKKEGRPDDTEPVIKSRLDTYHSQTRPLIDYYR
                     KEGVLVEVPGEGTLEEVTSRILSPLEKIK"
     gene            2369..2587
                     /gene="infA"
                     /locus_tag="PLG78_07495"
     CDS             2369..2587
                     /gene="infA"
                     /locus_tag="PLG78_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNE22994.1"
                     /translation="MAKEEAIEVEGTVVEPLPNAMFRVVLDNGHKVLAHISGKMRMHY
                     IRILPGDKVVVELSPYDLTRGRITFRKK"
     gene            2591..2704
                     /gene="rpmJ"
                     /locus_tag="PLG78_07500"
     CDS             2591..2704
                     /gene="rpmJ"
                     /locus_tag="PLG78_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNE22995.1"
                     /translation="MKVRVSVKKLCTNCRVIRRGGVIRVICENPRHKQRQG"
     gene            2704..3087
                     /gene="rpsM"
                     /locus_tag="PLG78_07505"
     CDS             2704..3087
                     /gene="rpsM"
                     /locus_tag="PLG78_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNE22996.1"
                     /translation="MARIAGIDLPKEKRAVVGLTYIYGIGKSQAAKILGAAGVDENIK
                     VGDLDEKQVNAIRNYIAQNCRLEGDLRTELNLNIKRLMDIGAYRGLRHRMGLPANGQR
                     TRTNARTRKGVKKTVANKKKAPTKG"
     gene            3102..3482
                     /gene="rpsK"
                     /locus_tag="PLG78_07510"
     CDS             3102..3482
                     /gene="rpsK"
                     /locus_tag="PLG78_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNE22997.1"
                     /translation="MAVAQKKKEKRNVPRGKALIQASFNNTIITITDLQGNVLAWSSA
                     GHMSFKGSRKSTPYAAQMAARNASEKAKESGVREVDVFVKGPGIGRESAIRAISIEGI
                     HVRSIKDITPLPHNGCRPRKRRRV"
     gene            3525..4517
                     /locus_tag="PLG78_07515"
     CDS             3525..4517
                     /locus_tag="PLG78_07515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNE22998.1"
                     /translation="MNLKHLMKGLKRPRQVEFGHETTQPNYGRFVAEPFERGFGATVG
                     NSLRRTLMSSIEGTAITAVRIEGVAHEFSTIEGVVEDVTRIILNLKQLRATYDSEGRD
                     EPKIIHIEKRGAGQLTAADFAVDSSIQILNPELHVATLNEDANLIMDVQFERGRGYVP
                     SEILKKNIDEIGTIAVDALFSPVRRANLDVSETRVGQRTDYEKITLDIWTDGSIAPED
                     ALAQAAKILKEHLTVFINFQEEPEEELDETNELEENLRRNLEKHVEELELSVRSLSVL
                     KSLEIETLADLVRRNEEELQKSRHYSPNCVEEIRQKLAGLNLEFGMRDFFAPRD"
     gene            4529..5068
                     /gene="rplQ"
                     /locus_tag="PLG78_07520"
     CDS             4529..5068
                     /gene="rplQ"
                     /locus_tag="PLG78_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNE22999.1"
                     /translation="MRKRNKTKQLNRVSSHRKAMMRNMATSLFDHERIISTRAKVKVL
                     RPYAEKLITRAKHTVSGEMNEAQVVHSKREILKHVRDRDVAVKLLQDIAPRFKERNGG
                     YLRIIHLPERDKDSAKMSIIELVERKEKVRKQKPEKERKASSSPEKGKDKKSLPSEKD
                     AKKEKWYNRFRKKKSDVPS"
     gene            complement(5101..5505)
                     /locus_tag="PLG78_07525"
     CDS             complement(5101..5505)
                     /locus_tag="PLG78_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE23000.1"
                     /translation="MRITSRGRYGLKAMMELSSGGLLKTREISERQNIPQKYLEQIVH
                     LLRKNGLVSSIRGAEGGYRLTRSASEITILEILEALEGDLSLIDRSDKTWDESTGGFW
                     KELESRLHEMLSVSLAEFMASGSKKGELMYYI"
     gene            complement(5505..6476)
                     /gene="gmd"
                     /locus_tag="PLG78_07530"
     CDS             complement(5505..6476)
                     /gene="gmd"
                     /locus_tag="PLG78_07530"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002148036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE23001.1"
                     /translation="MGKTALITGITGQDGSYLAEFLLSKGYKVFGMVRRSSSENFDRI
                     EHIRDKVILRQGDLLDQLSLLNLIEESAPDEIYNLAAQSFVPTSWTKPLLTGEFTALG
                     VTRMLEAMRAVKSKARFYQASSSEMFGMVQEVPQKESTPFYPRSPYGVAKVYGHFITV
                     NYRESYNMFAVSGILFNHESPRRGKEFVTRKVTDGVARIKLGLQTELKLGNLDAKRDW
                     GFAGDYVRAMWLMLQQDKADDYVISTGETHSVQELVEVAFSHAGLDWKKHVKIDPAFI
                     RPAEVDLLIGDASKAKKELGWVPEVSFEGLVKMMVDADIERLKNGRP"
     gene            6591..7106
                     /locus_tag="PLG78_07535"
     CDS             6591..7106
                     /locus_tag="PLG78_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23002.1"
                     /translation="MLQGKGVEKMGIEARIAERAESRHYDDADVKRVDSLSLSPLDSG
                     KWGFDAAATSELFQKPVPAPDFRSWKFRFIRGPLKPLARKIYVTLAQMFDRMSENKTQ
                     AFYDVVHELADLRRENAQLKERIQALEAKSRQDSRDPAVPSAENPRPPRPVERGAPGI
                     SSRSGNRPRPL"
     gene            7113..>7683
                     /locus_tag="PLG78_07540"
     CDS             7113..>7683
                     /locus_tag="PLG78_07540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE23003.1"
                     /translation="MDEQVLHSLQWIESMPSWVMFAFFFFSAFMENVFPPYPGDTILA
                     FSGFLAARGVMSLPIVFFAGFAGSVAGAFVMYMLGHRALELARRMERSVSRPAWLKNS
                     LHEAVSQESIEKTGLWFNRYGLYFVIVSRFSAGVRFFVSIVAGISRVSLPLYMLCFAL
                     GVVLWNGLLTAGGYTLGRNWELVLVYLKLY"
BASE COUNT         1905 a         1888 c         2000 g         1890 t
ORIGIN      
        1 cgtgccggct acaagaagaa gcttggcttc gaaggtggac agaacccact tcgtttgcgt
       61 attccaaaac gcggatttac caatattttc gcgactcgct accaggtggt aaacctcaag
      121 gacctgacag aggccggctt cagcggtgaa gtgaatccag aagcactcaa aggcaaaggt
      181 ttgatttcac gccccggtat gcctgtgaag atcctcggag tgggaaccgt gtcgggtaag
      241 ctgtctgtta cagcggatgc attctctgcg actgcgaaag cagccattga aaagtccggt
      301 ggaacctgca caataaggcc tgattacatt cctcgcgtag accgtaagtt cgagaaaaaa
      361 gcccagcgca aaacgcgcct tggtaaggga cgtgcatgaa cgcactgatc aacatcttca
      421 agatccccga gctccgtcgt aagattcttt ttactgcggg gcttgtgatc gtttatcgcc
      481 ttgggtcctt cattacgatt ccaggcgtca atccgatcgc agtggctcag tcacgcccgc
      541 caggccagtc attcctggac gttgtgaatg tgttctcagg cggggcgctc tttaagttgt
      601 cgatcttctc tctggggatc atgccctaca tttccgcctc catcatcatg agccttctga
      661 cagttgtgat tccacagctg gcaagaatgc agcgtgaagg agaagacggg cggcgcaaga
      721 tcaatcagta cactcgttat ggaacggttc ttctctgtgc aatccaggcg ctgtttatcc
      781 tggtctgggc tgtagatcag aagaatccaa acaacggtga gccgtttgtt cgtatggcgc
      841 gtacgtggtt cttcgtcgtt ggcctcgggg caatcactac aggcaccatt ctacttatgt
      901 ggatgggtga acaaatcaca gagcgcggca tcggaaacgg tgcatctctg attattttct
      961 ccggtatcat tgcgcaactg cccagcaagc ttgcgcagat gatgaaagac aaaagcattt
     1021 ctccttttga tattgtcatc ctggtctctt tgttcttgat tctaatcgcg cttaccatcc
     1081 tgctttcgca aggtgtgcgc aagatccctc tccagtatgg aaagaagatg cagggaaggc
     1141 gtctggttca ggcaagaagc cagagcattc agttcaaact gaactccgcg aacgtaatgc
     1201 caatcatctt cgcttcgtct cttcttcttt tccctcagac tgtaattgga atgctcggcg
     1261 aagactcacg cggatggatc ggttggcgca ttctccagga ttggctcaat ccttttgcgc
     1321 ccgagttcta caaacagctc ccgtattaca ctatttacac aattctcatc atcttcttcg
     1381 catacttcta cactgcgatc tatatcaatc cgcaggagct ttcggagaac ttgaagaagt
     1441 acggtggatt tgtaccgggg attcgcccag gaactcacac gaaagaatat gtagagtcta
     1501 ctctgaatcg catcatttta ccgggcgcga tctttctggc gggattggcc cttgctcctt
     1561 atttgatcat caactttctc ggatttaagg cgaatgcgaa catccagggt cttgcatata
     1621 cgttcggcgg gacatccctg ctgatcatcg tgggtgttgc actcgatact ctgaagcaaa
     1681 tcgagtcgca gctgatcatg cgcaactacg acggcttcat gaaaaagggc agaatcaagg
     1741 gaagatagtc ttatgcgcat cgtcttcatg gggcctccgg gcgcgggaaa aggaacgcag
     1801 gcgaagatca tttgtgatcg ttatgggatt ccgcaggtgt ctaccggtga gattctccgt
     1861 gccgccgtaa agaatggaac ggacatgggc aagaaggcga aagagtatat ggacgcaggc
     1921 aaactcgtcc ctgactccgt tgtgattgga attgtgaagg accgcattcg cgaaccggac
     1981 gcacaaaagg gttacatcct ggatggtttt cctcgcactc tcgagcaggc ggatgcgctc
     2041 aaagcgatgc tcgctgaaat caaaactcca ttgcagatcg cactgaatct ggacgttcgt
     2101 gacgacctgc tcattgccag actcctggat cgcgccaaga aagagggtcg tccggatgat
     2161 acggaacccg ttatcaagag tcgcctggac acatatcaca gccagacgcg gccgctcata
     2221 gactactatc gcaaagaagg cgtgctcgtt gaggtacccg gagagggcac tctagaagag
     2281 gtaacgtcaa gaattctttc cccactggag aagattaagt gagacttgac agggcaggtc
     2341 gttcctcaag tctgacaatt tcttaaccgt ggcaaaagaa gaagcaatag aagtagaagg
     2401 aaccgttgtg gaacccctcc cgaatgcgat gtttcgcgtc gttttggaca atgggcacaa
     2461 ggttcttgct cacatttccg gtaagatgcg catgcattac attcgcattc tgccaggcga
     2521 caaagtggta gtggagcttt ctccctacga tttgacgcga ggcagaatca cgttcaggaa
     2581 gaagtgacct atgaaagtta gagtatcggt aaaaaaactt tgtaccaatt gtcgggtgat
     2641 ccgacgcggt ggtgtgattc gcgtcatttg cgaaaaccca cgtcataagc agaggcaagg
     2701 ataatggctc gtatcgcggg aattgatcta ccaaaagaaa agcgggctgt tgtaggcctg
     2761 acttacattt atggaatcgg caaaagccag gctgcaaaga ttctcggagc agccggtgta
     2821 gatgagaaca tcaaggtcgg agatcttgat gaaaagcaag tcaacgccat tcgcaattac
     2881 atcgcgcaaa actgccgcct cgaaggggac cttcgcactg aattgaacct gaacatcaag
     2941 cgtctcatgg acattggtgc ttacagaggc ctccgtcatc gtatgggtct tcctgcaaat
     3001 ggtcagcgca ctcgcaccaa tgcacgcact cgcaagggtg tgaaaaagac tgtggcaaat
     3061 aagaagaaag caccaacgaa aggttaatcg gaatttagaa tatggctgta gcacagaaga
     3121 agaaagaaaa gcgcaatgtt ccacgtggga aggcgctgat tcaagcgtcg ttcaacaaca
     3181 cgatcatcac aattacagac ctgcagggca atgttcttgc gtggtcttcc gcgggtcaca
     3241 tgagcttcaa aggctctcgc aagtctacgc catacgcagc acagatggca gcccgcaatg
     3301 catctgaaaa agcaaaagaa tcaggtgtgc gcgaagtaga tgtattcgtg aaaggtccag
     3361 gaattggtcg cgaatctgca attcgagcca tctctatcga aggcattcat gtacgatcca
     3421 tcaaggacat cactccactg ccacacaatg ggtgtcgtcc tcgcaaacgt cgtcgcgtct
     3481 aatcaccctc gattttcgaa aatttggaag gagacaagcg taccatgaat ttgaagcatt
     3541 tgatgaaagg cctgaagaga ccccgtcagg ttgaattcgg ccatgaaaca actcagccga
     3601 actacggacg ctttgtggcg gaaccattcg agcgcggatt tggtgccacc gttggaaatt
     3661 ctctccgtcg tactctcatg tcttctattg aaggaactgc aatcactgcg gttcgtattg
     3721 aaggcgtggc tcacgagttc agcacaattg aaggcgtggt agaggacgtt acacgcatta
     3781 tcttgaatct gaagcaactt cgcgccacat atgattcaga aggccgcgat gagcctaaga
     3841 tcattcatat tgaaaagcgc ggagcaggac aactgactgc ggctgacttt gcagtagatt
     3901 catccattca aattctcaat ccagagcttc acgtagccac tctgaacgaa gatgcaaacc
     3961 tgatcatgga cgtgcagttt gagcgcggcc gcggatatgt tccatctgaa attctcaaga
     4021 agaacattga tgaaatcgga accatcgcag tggacgctct gttctcgccg gttcgtcgcg
     4081 cgaatctcga tgtatcagaa acacgcgtag gacagcgcac tgactacgaa aagatcactc
     4141 tggacatatg gacagacgga tcgattgcgc cggaagatgc actggctcag gcagccaaga
     4201 tcctcaaaga acatctgact gtatttatta acttccagga agagccggaa gaagaactcg
     4261 atgagacaaa tgaactcgaa gagaaccttc gccgcaatct ggagaagcat gtagaagaac
     4321 tcgaactttc tgttcgctct ctttctgtgc tcaagagcct ggaaatcgaa actctcgcgg
     4381 atctggtccg cagaaacgaa gaagagcttc agaaatcacg tcactattcc ccgaactgcg
     4441 tagaagaaat tcgccagaaa cttgcgggac tcaatcttga attcggaatg cgcgactttt
     4501 ttgcgcctcg cgactaaggc ctgacatcat gagaaagaga aacaaaacaa aacaactgaa
     4561 tcgcgtatct tcgcacagaa aagcgatgat gcgcaacatg gcaacgagct tgtttgacca
     4621 tgagagaatc atttctacgc gtgcgaaggt aaaagtcctt cgtccgtacg cggaaaagct
     4681 gatcactcgc gcgaagcaca ctgtgtccgg cgaaatgaac gaagcgcaag tggttcacag
     4741 caagagagaa attctgaagc acgttcgcga tcgcgacgtg gctgttaaac ttcttcagga
     4801 tatcgcgcct cgtttcaagg aacgcaacgg tgggtacctg cgcatcattc atcttccaga
     4861 acgcgacaaa gatagcgcga agatgagcat catcgaactg gtggaacgca aagagaaggt
     4921 tcgcaagcag aaacctgaaa aggaaagaaa agcttcatcc tcaccggaga aaggtaagga
     4981 caagaagagc cttccaagcg aaaaagacgc caagaaagag aagtggtaca atcgcttccg
     5041 taaaaagaaa tcagacgttc caagctgatt ggagaaaggc cgcgaaagcg gccttttttg
     5101 ttagatgtag tacattagtt cgcctttttt agaaccagag gccatgaatt ctgccagaga
     5161 tacgctgagc atttcatgaa ggcgagactc cagttctttc cagaagccac ccgtagattc
     5221 atcccatgtc ttgtctgagc gatcaatgag ggatagatct ccttcgagag cttcgagtat
     5281 ttcaagtatt gtgatttcag aagcagagcg agtgagtctg taaccgcctt ctgcgccgcg
     5341 gatgctcgat accagcccgt tctttcgaag gaggtgcacg atttgctcga ggtatttttg
     5401 cgggatgttc tgcctttccg agatttctct tgttttcaag agcccgcccg acgacaattc
     5461 catcatcgct ttgaggccgt agcggccgcg gcttgtgatt ctcactatgg ccggccgttt
     5521 tttagtcttt caatatccgc gtcgaccatc atcttcacga ggccttcaaa gctcacttct
     5581 ggaacccagc cgagttcctt ttttgccttg gatgcatctc cgatgagtaa atccacttcg
     5641 gcgggacgaa tgaatgcagg atcgatcttc acgtgcttct tccagtccag gcctgcatgg
     5701 gagaatgcta cttctacaag ttcctgcacg ctgtgtgttt ctccggtgga gattacgtag
     5761 tcgtccgctt tatcctgctg gagcatgagc cacatggcgc gcacgtaatc tccggcaaat
     5821 ccccagtcgc gtttcgcgtc caggtttcca agcttgagtt ccgtttgaag gccgagtttg
     5881 atacgagcta ctccgtctgt caccttacgc gtaacaaatt cctttccgcg gcgaggggat
     5941 tcgtgattga acagaatgcc tgaaaccgcg aacatattgt aggattcgcg gtagttcaca
     6001 gtgataaaat gcccgtacac ttttgcaacg ccgtaagggc tgcgaggata gaaaggtgtg
     6061 ctttcttttt gagggacttc ctgcaccatt ccaaacattt ccgagctcga tgcctggtag
     6121 aatcttgctt ttgattttac tgctctcatt gcttcgagca tacgagtcac accgagtgca
     6181 gtgaattctc ccgtcaggag tggcttcgtc caggaggtcg gcacaaagga ttgcgctgcc
     6241 aggttgtaga tttcatccgg ggcggattct tctattaaat tgagaaggct gagctggtcg
     6301 agcaggtctc cctggcggag gatcactttg tcgcgtatgt gctcgattct gtcgaagttt
     6361 tcgctcgatg agcggcgaac catgccaaaa accttgtagc ctttcgaaag caagaattct
     6421 gcgaggtagg aaccgtcctg gcccgtgatt cccgtgatca atgccgtttt acccacgatt
     6481 gcgctccttg ggccagattc gccaaattcg ctatttttac cattcctttt gcttgttgtc
     6541 ctggaagcaa aaatgagtgc ggtccccggc ctgcgagcaa tggtggcctc atgcttcagg
     6601 gcaaaggcgt ggaaaaaatg gggattgagg ctcgaattgc ggagagagct gaatctcgcc
     6661 attatgacga tgctgatgtc aagcgtgtgg attccctttc tctatctccg ctcgattctg
     6721 ggaagtgggg ttttgatgcc gcggcaacgt cagagctttt tcaaaagcca gtccccgctc
     6781 cggattttcg tagctggaag ttccgcttca ttcgcggtcc tctcaagcca cttgcccgca
     6841 agatctacgt tactctcgca cagatgttcg atagaatgtc tgaaaataag acccaggcat
     6901 tttacgatgt tgtgcacgag cttgcggacc ttcggcggga gaacgctcag ctgaaggagc
     6961 gcattcaggc actcgaagca aagtcgcggc aagactcgcg agatcctgct gttccttcag
     7021 cggagaatcc tcgcccgcca cggccggtcg agcgcggtgc gcctgggatt tcttcaagga
     7081 gtgggaatcg tccgcggcct ctctagttgt gcatggatga gcaggttctt cattctcttc
     7141 agtggatcga aagcatgcct tcctgggtca tgtttgcatt cttctttttt tctgcgttca
     7201 tggagaacgt ttttcctccg tatcccggag acacaattct agcattctcg ggctttcttg
     7261 ccgcccgcgg agtgatgtcc ttgcccattg tattctttgc tggattcgcg ggcagtgtgg
     7321 ccggcgcatt tgtgatgtat atgcttggac acagggctct cgaacttgcg cggagaatgg
     7381 aacgcagcgt ttcgcgtccg gcctggttga agaacagtct gcatgaagcg gtttcgcaag
     7441 aaagcatcga gaagacgggt ctgtggttca atcgctatgg tctttacttc gtcatcgtgt
     7501 cgcgattttc tgcgggagtc cgtttttttg tgagcatagt ggcgggaatt tcgagggtct
     7561 ctctgcccct ctacatgctt tgcttcgccc tgggtgttgt gctgtggaac gggcttttga
     7621 cggcgggagg ctacactctc ggccgcaact gggaactcgt acttgtatat ttgaagttgt
     7681 aca
//
LOCUS       DAOQJL010000129         7666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5211_length_7666_cov_3.650374, whole genome shotgun sequence.
ACCESSION   DAOQJL010000129 DAOQJL010000000
VERSION     DAOQJL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_7666_cov_3.650374"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLG78_07545"
     CDS             complement(<1..693)
                     /locus_tag="PLG78_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23004.1"
                     /translation="MKSRMIQTACLILLPFASFAEKSHEQMGDAYYKKKDFGLARIEY
                     DRAILDSSNESVRAKIGLTLIRERRFRQAIESLQGKSFDIMYFRMYGDLRANFRNRFL
                     MDQGELEESKLPESKKDLGRLLAGTLYLEDGDFDRARIHFEKLQRETENERVRIAASE
                     TLTAMTTYNEIPRKSVWLAAGLSALLPGSGQVYSRNVADGLTAFAFTSVLCGSAAYMN
                     HLETRARSGHGGS"
     gene            746..1054
                     /gene="yidD"
                     /locus_tag="PLG78_07550"
     CDS             746..1054
                     /gene="yidD"
                     /locus_tag="PLG78_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE23005.1"
                     /translation="MFRPMLYLIDRHREEFTHYDGPRCPMYPSCAAYADRAIRQYGFL
                     GALMFANRLFFAESGDLASKYVLVPHRMSEMPRYYDPVSDDILPGEHRPSLLMEDFSE
                     "
     gene            1095..3011
                     /locus_tag="PLG78_07555"
     CDS             1095..3011
                     /locus_tag="PLG78_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE23006.1"
                     /translation="MGILKTLAPLLDHPDYSGARDPFYFSRAIRSLRSPLDLPDMEKG
                     IAMLRSVIEAQGHVLVVGDRDVDGVSSAALLANFLRDQHAKRGGGLEIRVSDLGDDYG
                     LSGELFENVRDGKADLVILLDMGSSHGPEIHELIAAGKKVIVLDHHQVSDRRPSHDLC
                     AFINPQLNAEVHENEGKIPTVGLAFKFLFGYALSHIAEWNNHSLVDLSFLFSENANAG
                     ARFAVYRCGCLVESGENISGAHLRPLDADGMAFLKHERERILQSEKPVVTIGSLLLSR
                     IVETRPRLLDFLMSLSDLVSVGMITDMVPLAGENRSLVRIGLGMAGKRVRRGVPGYRA
                     LIGALDLPEFDLTGRDLAWSIGPAINAAGRMGNTALALDLLTSDSPVEAERLARELKA
                     LNKKRQERTRSNERIVTEHFEAYPEKTDRPIIFCYHESLEPGVSGIMATRLLEKYGKP
                     VVYINPDGKWAKGSARGEGHNVLQMLDCAAHLFIQFGGHPEAAGFSLPYERIEELERV
                     LLENAPFMDLEPVSLEIRPHLDLLPEQINRRLYDELRQMEPFGPGNPEPLIRITGAQV
                     TDVTYMKDKLHARFRIRGSRDIEFVAWRRGTEFGKDMERVDLIGSLERSIFAGRTRIQ
                     FRVEHVKAAAVLVA"
     gene            complement(3045..3566)
                     /locus_tag="PLG78_07560"
     CDS             complement(3045..3566)
                     /locus_tag="PLG78_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE23007.1"
                     /translation="MSEHSSTSQQKGPSKHGLSWIYFAIAFTFVAGTAYAIFYHGFLG
                     ATNATTYRESVGMSVVAVTEDFIPERTPQAIANGAASYKTCAACHGANLEGLACPNLA
                     DNEWLHFKEPKETNLAKLIKGGVPAGATKGPKGGAMPPMGTLDNMTKVWEVIYYLQTK
                     NPNIIKDSVPTKK"
     gene            complement(3582..4238)
                     /locus_tag="PLG78_07565"
     CDS             complement(3582..4238)
                     /locus_tag="PLG78_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNE23008.1"
                     /translation="MSRIYEKLFSSPLSTVLSSIAIVIVGYGIAVCLPFSENISHGSL
                     SQLSTEDTLVLKGHEVYYQEGCQYCHSQNLRPFGWEVSRYVNVEKYGYYPVPTAMEYS
                     HETPAMRGSFRIGPDLSRVAGKQDAAGLTSLLRGGKEDNLRNRYHAYGYLFNDDDLQP
                     LFLSWKIRMMLEAGVPLSDPYHKSAFDRLEGQTRGDALVAYLLSLGQKSMQYNGAFYR
                     "
     gene            complement(4238..5695)
                     /locus_tag="PLG78_07570"
     CDS             complement(4238..5695)
                     /locus_tag="PLG78_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE23009.1"
                     /translation="MIKLTPDFRKVLSFHLGGGVIWTLIAIVFGLLNQLQLMLGWPQP
                     DVLGYGLIRPLFTTSLIFGGIVNLSLAGSYYVMQKSGDLAWQPLALAGFGLQQLGLLL
                     GVIGILLGANKGREYGEMNWVSDHLFFLSLVFFIAVALRSLKKELNAPVLFILAGAAG
                     GAISFMLGNLALPYSPMGEVPLTAGMKDQAVQEFYRISVLGYFVLFPAFGLLYHFIPT
                     HYKLPIYNEGAVRFQALAMLALIPLSGAAYLAYGAGPQMSATFGIVCGIALNFAVIMG
                     AANLLYTVSRGPSRVWSNLDGSYLVWGVRFILILAILRAISLPRFMQADVGYTWWNSR
                     DISQDALVAASVVFAGLAYGYSQRFNQKAAANTLASAALLLAVVGSCLMIVAGTVQGI
                     LGGMSARAMTEAGPAVTSFSALLFAGNVAGDTASPTLKFILGLRGIYFLGYASVTVSL
                     ATWALSMLSIWRGQGNPFQEADLHLPDEENPEEAR"
     gene            complement(5692..6600)
                     /locus_tag="PLG78_07575"
     CDS             complement(5692..6600)
                     /locus_tag="PLG78_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE23010.1"
                     /translation="MPALASIEGITLKSLIITPAAIALFLIGCGQKTPPVYESSQDQI
                     FLGSVMYSQENCKNCHGNAWDGKGPDAESLGVPVPNFTSAVGPEKTPTSYFKAITVGT
                     DKVKNHAYQSLTDKGRWALAHFLYSLKKPLAGEKADVQRKNIEAGMTEARDAYAKTRR
                     WNMGFQPLDQREKAPDLAELVKIADLKPEGGPGAVSDERKARPHNGEGASLYKNNCES
                     CHGIYGEGRNLTFGMGLIASKNDFYGFKKTRATISTRDLASSGSTSAGGLKSAHGSGS
                     LLLNNFDSFTDAQWSALSDFIRGMAQ"
     gene            complement(6579..>7666)
                     /locus_tag="PLG78_07580"
     CDS             complement(6579..>7666)
                     /locus_tag="PLG78_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23011.1"
                     /translation="SLLLALLVALPLGLGGLYFVAFNHLAGAAWCVTIRRIAESYFPY
                     LLVVLALMAAVFFGMKNVYSHWMLTTEGGHADIADHLLQVKRPWLNPGFFVTRNFAFV
                     LLWLGFGFVLWKKSTDQDTDGQVEHTRSLAKWSAVFVILFALTYSASAWDLTMSLEPH
                     WFSTMWGPYCFAGLALTAFSSLILWIWYLKSSGYFGDALNENHYHDMGKYMWGHSIFW
                     AYVMICQYLLIWYAHIPEETIFYRKRIYTPEIGFQPWAIVSLLLILVRFVIPFFFIIQ
                     RKAKRNIKHLGIGACIVLLGQVIDMYWVTYPTLANGDFVMFDLRELGTISLVAGSFIL
                     VTAWSLSRKSLVPKGDPRLEECLHWHQ"
BASE COUNT         1847 a         1909 c         2028 g         1882 t
ORIGIN      
        1 ggaaccgcca tgtcccgaac gagcgcgcgt ttccagatga ttcatgtatg ccgccgatcc
       61 gcaaagtacg ctcgtgaatg cgaaagcggt gagaccatct gcaacgttgc gcgagtaaac
      121 ctgacccgat cccggaagca aggcggaaag cccggctgca agccatacgc ttttgcgcgg
      181 gatttcattg taggtggtca tggctgtgag cgtttcgctt gcggcgattc tcactctttc
      241 attttccgtt tcgcgctgga gtttttcgaa atgaattcgg gcacgatcaa agtcaccgtc
      301 ttccaggtat agagttcccg ccaggagtct ccccagatct ttctttgact cagggagctt
      361 gctttcttct agttctccct ggtccatgag aaagcgattt cgaaagttcg ctcttaaatc
      421 gccatacatg cgaaagtaca ttatatcaaa tgattttccc tgaagagatt caattgcctg
      481 gcgaaacctt ctttcgcgaa ttagggtcag gccaattttt gcgcggactg actcattgct
      541 tgaatcgagt atcgcgcggt cgtattcgat tctggccagt ccgaagtctt tctttttgta
      601 gtacgcatct cccatttgtt cgtgactttt ctcagcgaag cttgcaaagg gtagaagaat
      661 gaggcacgca gtctggatca ttctgctttt catgacagtt tgcaaatcgg cacagagtcc
      721 cgcggccggc gaggaaaatc cgatcgtgtt tcgtcctatg ctgtatttga ttgatcgcca
      781 ccgcgaagaa ttcactcact atgacggtcc acgttgtccc atgtatcctt cgtgtgcggc
      841 ctatgcggat cgggccattc gccagtatgg ctttctgggc gccctgatgt ttgcaaacag
      901 gcttttcttc gcagaaagcg gagatctagc atccaaatac gtgcttgtgc ctcatagaat
      961 gtctgaaatg cctcgatact acgatccagt gtccgatgac atccttccag gagagcacag
     1021 gccgtctctt cttatggaag acttctcaga atagttgacc ggattctgtc tcatagacat
     1081 aatacccttc agtcatggga atactcaaaa cactggctcc gcttctggac caccctgact
     1141 actcgggcgc ccgcgatcct ttttactttt ctcgcgccat tcgttctctg cgttcccctc
     1201 tggatttgcc ggatatggag aagggaatcg ctatgcttcg atccgtcatc gaagctcagg
     1261 ggcatgttct tgttgtggga gacagagacg tggacggcgt gagttctgcc gcacttcttg
     1321 cgaatttcct gcgagaccag catgcgaaaa gaggaggcgg acttgagatt cgtgtctctg
     1381 atctgggaga cgactacgga ttgtcgggag agctttttga gaatgtgcgc gatggaaaag
     1441 cggacctcgt gattttgctc gatatgggtt cttcacacgg gccagagatt catgagctca
     1501 ttgctgccgg aaagaaagta attgttctcg atcaccatca ggtttccgat cgcaggcctt
     1561 cgcatgatct ttgcgcgttc atcaatccac aattgaatgc agaagttcac gaaaatgaag
     1621 gcaagattcc aaccgtcggc cttgctttta agtttctatt cggctacgca ctttctcaca
     1681 tcgcagagtg gaacaatcat tctcttgtag atttgtcgtt tttgttttca gaaaatgcga
     1741 atgcaggcgc caggtttgca gtctatagat gcggatgcct tgtggaaagc ggggagaata
     1801 tttctggagc acaccttcgt ccactcgacg cggatggaat ggcgtttctc aaacacgaac
     1861 gcgaaaggat ccttcaatct gaaaaaccag tcgttactat tggttctttg cttttgtcgc
     1921 gcattgtgga gacccgtccg cgccttcttg attttttgat gtctctatct gatcttgttt
     1981 ctgttggaat gatcacggat atggttcctc tggccgggga aaacagatct cttgtgcgaa
     2041 tcggtcttgg gatggcagga aaaagagtga ggcgcggagt tcctggttac agagcgctca
     2101 ttggtgctct tgatttgcca gagtttgatc tgacaggtcg ggacctggcc tggtccattg
     2161 gtcctgccat caatgctgcg ggcaggatgg gaaacacggc acttgcactg gatttactca
     2221 ccagcgactc tccggtcgaa gcggagcgcc tcgcacgtga gctcaaagca ctcaacaaaa
     2281 aaagacagga gcgcaccagg tccaacgagc gcatcgtgac ggaacatttc gaagcgtatc
     2341 cagagaagac agatcgtccc attatattct gctatcatga aagcctggag ccgggtgttt
     2401 ctggaattat ggccacaagg cttcttgaaa agtacggcaa gcctgtagtc tacatcaacc
     2461 cggacggaaa atgggccaaa gggtctgcca gaggggaagg gcataatgtg ctccagatgc
     2521 tggattgcgc ggcgcatttg ttcatacagt ttggcggtca tccggaagcg gccggttttt
     2581 ctcttcccta tgaaagaatt gaagaactgg aaagagttct actggagaac gcacctttca
     2641 tggatctcga accggtcagc cttgaaatca ggccgcatct ggatctcttg cccgaacaaa
     2701 tcaaccgtcg tctttatgat gagctcagac agatggagcc attcggcccc ggcaacccgg
     2761 aaccactcat tcgcataacg ggtgcgcagg tgacagacgt aacttacatg aaagacaaac
     2821 tgcatgcgcg ctttcggatc cggggaagtc gggacatcga atttgtagcg tggcgtcgcg
     2881 ggacggagtt cggcaaggat atggaaaggg tggatcttat tggttcactg gagcggagca
     2941 tttttgcagg cagaacgcgg attcagttca gagtggaaca cgtgaaagcg gccgcagttc
     3001 tggtggcgtg atcaggcggc gccaatgccg cctgacgttg aaatttactt cttggtagga
     3061 actgagtctt tgatgatgtt cgggttcttg gtctgcaggt agtagatgac ttcccacacc
     3121 ttggtcatgt tatccagagt ccccatagga ggcatcgcgc cacccttcgg gcctttggtt
     3181 gctcctgcag gaacgccgcc tttgatcagt ttggccaggt tggtttcctt tggctctttg
     3241 aagtgaagcc attcgttgtc ggccaggttt ggacaggcaa gaccttcgag gtttgcaccg
     3301 tggcaggcgg cgcacgtttt ataagacgcc gctccattcg cgatcgcctg aggagtgcgc
     3361 tcaggaatga aatcttctgt cacggcaaca acggacattc ccacagactc gcggtatgtg
     3421 gttgcgttcg tcgcgccaag gaagccgtga tagaagatcg cgtatgctgt ccctgcaaca
     3481 aaagtaaatg caatcgcaaa gtaaatccag gatagaccgt gtttgctcgg tcccttttgc
     3541 tgcgaggttg atgaatgttc gctcataatt ataccttagc ctcagcgata aaatgcgccg
     3601 ttgtattgca tgctcttctg tccaagactc agaagatagg ccacaagcgc gtctccgcgt
     3661 gtttgtcctt ccaggcgatc gaaagcactc ttgtgatatg gatctgaaag cggaactccc
     3721 gcttcgagca tcatgcgaat cttccacgaa aggaacagcg gctggagatc atcgtcgttg
     3781 aacagatagc cgtaagcatg atagcgattg cgcaggttgt cctctttccc accgcgtagc
     3841 agggaagtca ggccagccgc atcctgtttg ccagcaacac gcgaaagatc tggaccaatt
     3901 ctgaaacttc ctcgcatagc aggtgtttca tgcgaatatt ccattgcagt tggaaccgga
     3961 tagtagccgt acttttcaac gttcacatag cgtgacactt cccagccaaa tggtctgaga
     4021 ttctgagaat ggcaatactg gcagccttcc tggtagtaca cttcatggcc tttgagaacc
     4081 agagtgtctt ctgtgctcaa ttgcgagaga gagccatgag aaatgttttc gctgaaaggc
     4141 aggcacactg cgatcccata cccaacgatc acaatggcga tcgaagatag tacagtgctc
     4201 agtggactgg agaaaagctt ttcgtaaatg cgggacatca gcgtgcctcc tctggatttt
     4261 cttcatccgg aaggtgaaga tctgcttcct ggaatgggtt tccctggcct ctccagatcg
     4321 acagcatgct gagcgcccag gtggcgaggc tcactgtgac ggatgcgtaa cccaggaagt
     4381 agattccacg gagtccaaga atgaatttca aagtggggga tgcagtgtct cctgcaacat
     4441 tgcctgcaaa caggagtgca gaaaaggaag tcactgccgg gccggcttct gtcatcgcgc
     4501 gggcagacat gccgcccagg attccctgca ctgtgcctgc cacgatcatc aggcagcttc
     4561 ctactactgc aagaagtagt gcggcactgg ccagtgtgtt tgccgcggcc ttctgattga
     4621 atctctgtga atagccatac gccaggccag cgaatactac agatgcagca acgagtgcat
     4681 cctgtgaaat atcgcgggag ttccaccagg tgtatcctac atccgcttgc atgaagcgcg
     4741 gaagagaaat ggctcgaaga atggccagaa tcaaaatgaa tcgcacaccc catacaagat
     4801 aggatccgtc cagattggac cagactctgc ttggtccgcg tgaaactgta tagagcaggt
     4861 tggcggctcc catgatcact gcaaagttca gtgcaattcc gcatacaatt ccaaacgtcg
     4921 ctgacatttg cgggcccgcg ccataagcca ggtaggctgc gccagagagt ggaatgagtg
     4981 cgagcatcgc cagcgcctgg aaacgcaccg cgccttcatt gtagatagga agcttgtagt
     5041 gagtaggaat gaaatgatac aggagaccaa acgccgggaa aagaacgaag tatccgagca
     5101 cgctgattct gtagaattcc tgcactgcct gatctttcat tccagctgta agcggcacct
     5161 cgcccattgg actgtagggc agggccagat ttccaagcat gaagctgatc gctccgcccg
     5221 ccgctccggc gaggatgaag agaacagggg cattcaattc ttttttcaaa gatcgcaacg
     5281 ccactgcaat gaagaagacg agagatagaa agaacagatg atcagaaacc cagttcatct
     5341 ctccgtattc tctacctttg tttgctccca ggagaatgcc gattactcca agaagcagac
     5401 ccagttgctg cagtccaaag cctgcgagcg ccagtggttg ccaggcaaga tcgcctgact
     5461 tctgcattac atagtaagaa ccggccagag agagattcac aattccgcca aagatcagag
     5521 aagttgtgaa aagtgggcgg atcagtccgt atcccagaac gtctggctgc ggccagccaa
     5581 gcatgagctg gagctggttg agcagaccaa atacaattgc aatcaaagtc cagatcacac
     5641 ctccgccgag gtgaaacgaa aggacttttc taaaatcagg tgtaagctta atcattgcgc
     5701 catacctcga atgaagtcgc tgagtgcgct ccactgagca tctgtgaagc tgtcgaaatt
     5761 gttcagaaga agactgccgc taccgtgagc cgacttgagg ccgccagcgc ttgttgagcc
     5821 ggagcttgcc agatcgcgag tgctgatcgt cgcgcgagtc ttcttgaagc cgtagaaatc
     5881 attcttggaa gcaatcagtc ccattccaaa tgtaagattg cgtccttcgc catagatgcc
     5941 gtggcagctt tcgcagttgt tcttgtagag gcttgcacct tcaccgttat gcggtcttgc
     6001 tttgcgctca tcgctcacag cccctggtcc accctcaggc ttgagatctg cgattttgac
     6061 gagctctgcc aggtccggag ctttttcgcg ctggtccaga ggctggaagc ccatgttcca
     6121 tctgcgagtc ttcgcgtacg cgtcgcgcgc ttctgtcatg ccagcttcta tgttcttgcg
     6181 ctgcacgtct gctttttcgc cagcgagcgg cttcttcaga gaatagagga aatgtgcgag
     6241 tgcccagcga cctttgtctg tcagagactg gtaggcgtgg tttttgactt tgtctgtgcc
     6301 gactgtgatt gctttgaaat aagaagttgg agtcttttca ggtccgaccg cagaagtgaa
     6361 atttggaacc ggcactccaa gtgattctgc atccgggccc ttaccatccc aggcatttcc
     6421 atgacaattc ttacagtttt cctgcgaata catcacggaa cccaggaaga tctggtcttg
     6481 cgaagattcg tagaccggcg gtgttttttg gccgcagcca atcagaaaaa gtgcgatggc
     6541 tgcgggagta atgatcaacg atttcaatgt gataccctct attgatgcca gtgcaggcat
     6601 tcttccagtc ttggatcgcc tttgggcaca agagacttac ggctgagtga ccaggccgta
     6661 actagaatga agcttcccgc aacgagtgat atggttccga gttcgcgaag atcgaacatg
     6721 acgaagtctc cgttcgcaag ggttgggtaa gtgacccagt acatatcaat cacctggccc
     6781 agaagcacaa tgcatgcgcc aatgcccaga tgtttgatgt tgcgttttgc tttgcgctgg
     6841 atgatgaaga agaaaggaat cacgaagcgc accagaatca acagcaggct gacaatggcc
     6901 catggttgga atccgatctc tggagtgtag attctctttc tgtagaagat ggtttcttct
     6961 ggaatgtgtg cataccagat gagtaggtac tgacagatca tcacgtatgc ccagaagatc
     7021 gaatgtcccc acatgtattt gcccatgtca tggtagtggt tttcattcaa tgcatctccg
     7081 aaatagccgg agctcttgag ataccagatc cacagaatga gagatgagaa agcagtgagt
     7141 gcaagtcctg cgaagcagta agggccccac attgtggaaa accagtgtgg ttcgagactc
     7201 atggtcagat cccacgcgct tgcagagtaa gtgagtgcaa aaagaattac aaataccgct
     7261 gaccatttcg caagagatct ggtgtgttca acctgtccgt ctgtgtcctg gtcagttgat
     7321 ttcttccaga gcacaaatcc aaatccaagc cacaacaaca cgaatgcgaa gttacgcgtt
     7381 acaaagaatc cagggttgag ccagggtctt ttgacctgga gtaagtggtc cgcaatgtct
     7441 gcatgaccgc cctctgttgt tagcatccag tggctgtata cattcttcat tccaaagaag
     7501 actgcagcca tcagagcgag aaccacgaga aggtatggaa agtagctttc cgcgatgcgg
     7561 cgaatggtaa cgcaccacgc agcacccgct agatgattga aagcgacaaa atagagtccg
     7621 cccagaccga gcggcaatgc gaccagaagc gcaagcagca gagaca
//
LOCUS       DAOQJL010000130         7644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5226_length_7644_cov_5.006588, whole genome shotgun sequence.
ACCESSION   DAOQJL010000130 DAOQJL010000000
VERSION     DAOQJL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_7644_cov_5.006588"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLG78_07585"
     CDS             complement(<1..801)
                     /locus_tag="PLG78_07585"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNE23012.1"
                     /translation="MMSDAIDQLVKSASARDPDPLVIAGRAFASRLLVGTGKFSSPSI
                     MAEAVRASGTEMVTVALRRIDLSQKLEDQDLVSHLDRDRIVLLPNTSGARDAEEAIRL
                     ARLAREMGGGDWLKLEVTPDPVYLLPDPVETLKAAAVLVKEGFKVLPYINADPILALK
                     LQDAGCVTVMPLGSPIGSNQGIRTAESIRIIIEQARVPVVVDAGLGAPSHAALALEMG
                     AHAALVNTAIAVARDPVRTAEAFALAVIAGREGFLHGPSTGGGHDWKKA"
     gene            851..1945
                     /locus_tag="PLG78_07590"
     CDS             851..1945
                     /locus_tag="PLG78_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE23013.1"
                     /translation="MDPIPFEAPAFARNPHFQTIATHLMQKVPGYMPRVLGLVYETAV
                     VPTLDGNGDQLLLHVHRFHDHDQPAVLLVHGLEGSADSHYIVKMTDKLLAAGFHVVRM
                     NLRSCGAGRTLARTAYNCGLTIDVEAALKFMQEHVSRAIALVGFSLGSNLTVKYMGED
                     RSERDQQIIARKGKPIRTRIRDRIAEVFCAISIPLDLYASCEYLDSDNCRIYRDAFMA
                     DFKKKVESGKFDQIAGAREEIKNVKTWFDFDHYYTAPSTGFRSAVDYYASYSSRNFVS
                     RIDIPGLMLHARDDPLIHPGAWEETDWSGYKHLTAEITEHGGHLGWLGQKHPFFPDRR
                     WMDYRVLKYLLEWKDSRKLKTKRYSLFSGP"
     gene            complement(1921..2604)
                     /locus_tag="PLG78_07595"
     CDS             complement(1921..2604)
                     /locus_tag="PLG78_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23014.1"
                     /translation="MRLPKSALLLLVMVSCASRPSWVHSLPDHGGALGLRAVGLNLPA
                     GKWVRAAKNRFPYAHRTTYSDAREQIEISESGRYAKLHLYAAVSVDNEEFIAYEEEGT
                     VERSSGNWVLFRPEKSKTYQRSGPPVNVKMRRDQPYPFPAVSSIPFQEVSSGEPLLYL
                     VDEYHGKPVLTPAAYERLGKVFDFGIFQGSETMYDSQSRHFQDFRSKLFEKSFQPTHY
                     SYQGPEKSE"
     gene            2681..3601
                     /locus_tag="PLG78_07600"
     CDS             2681..3601
                     /locus_tag="PLG78_07600"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE23015.1"
                     /translation="MTARTGWKRRKKSDNLKYMTQASTDYQRIEKALCYIDSVFPAQP
                     PLAEIARHTGLSEFHFQKLFQRWVGISPKRFLQFITKDHSLRILKESRSILDAALDAG
                     LSGPGRLHDLLVECDAMTPGEIKSGGRNLLIRYGTTTSPFGLCMLAITDRGICRLEFI
                     ETRKDEKRIQNQWKQDFPEATFREDVVAADEMTLQIFGGKKADALSVLLNGTNFQIQV
                     WQALLRIPSGSVLSYEDVAQMIGRPEATRAVASAIAHNRIAFLIPCHRVIRKMGVIHK
                     YHYGTTRKKAMLGWEAGMKRPSLRNTQEEE"
     gene            3652..4476
                     /locus_tag="PLG78_07605"
     CDS             3652..4476
                     /locus_tag="PLG78_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNE23016.1"
                     /translation="MPVAYLGKKGSFASLVARQRYPDAETVSQPTIGDLVTYVKEGAD
                     RMGVVPIENSSGGIIFDTVDAFVDDSFNLFILEETRIRVRYALLGKSGQPIQTILSHY
                     AALRHCAAWIRSTYPAVRTQPVASTSEAAAMAAAGSQLAAISTRESANEYGLDVLHFP
                     IMDSVPNITQFFTIGHAPVNYSGSGGNTSLVATLDNSPGSLFRFLKPFHDLGVNLNRI
                     QSRTIPGDSSACKFFVGLEGVESETNVGRAIELARPLTVALQLLGSYPRLMDPYES"
     gene            complement(4484..6178)
                     /locus_tag="PLG78_07610"
     CDS             complement(4484..6178)
                     /locus_tag="PLG78_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE23017.1"
                     /translation="MARFCLLWLIPFVLHAGPALRVLEVDSSHFPNVEILAELGEGEG
                     TPQFTLRDGAPITPSITASRKEADRLVLAVVFDATGSIPAADYRRAQNGAGVFLSSLT
                     ESDRTALFQINGGSINISGFVPGGKTISEQIAQMKRVGRTTRIFDALHSGINAAREGI
                     LRNQNTESSSKQIAKAAVLLFTDGRDEASYLNEQDCLELAELGTKLRVPVYTVLYGHP
                     RNQRVFQRLSMRTGGKLITMLTPDTMRNLSRSIRLLKDETFRLSYKAPEGAGPRNIVL
                     SVKRGTTIDEDTASYNAPSVSIPHSEGPWYNSIPWGNVLLGVLSFLLFVAAVVALILV
                     LKQKHDAAKATNPAVVSASDSPTAHVKLPEGETEKVDIVEGIPAEDMPHPAVQYLREN
                     AYRSLQEALRGAPRYAAASLIRMKDGMSREYDLFLENTALGSGRWASIRVEDSGAAKV
                     HAKVKRVDSRYVLYDLMGGSSVFLNGKKLLRPRGLHDGDEIKLGRSVFQFRGKGKGNA
                     AAPDDRTETVQGKSNPASSMDSTQAMISDGSEDPADYSDTMPEEDLHDSDFLQKAE"
     gene            complement(6200..6463)
                     /locus_tag="PLG78_07615"
     CDS             complement(6200..6463)
                     /locus_tag="PLG78_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23018.1"
                     /translation="MIRDILYSIIYSGIGIYLVLIALGFLHRGVLGEMTGARRLAAWI
                     MALGFLGLGGHHAWQYYRFSSPEGRQILEKMKETEGNVIIGSP"
     gene            complement(6460..7425)
                     /locus_tag="PLG78_07620"
     CDS             complement(6460..7425)
                     /locus_tag="PLG78_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE23019.1"
                     /translation="MQSGHLGIDLGGSNIFSVVFDDSYHPVTQSKIDTEAKEGYNHVI
                     GRLRGQIDALIASCESKGIRIETIGLGVPGVVSKDTDLVRVAPNLNWENRHPLQDLGL
                     LVQRDIRSVLVNDVNAGLMGELARMQHKPSIAIAFFCGTGIGGAIAINGELVLGRDGG
                     AGEVGHIIVRDGGRKTDSGLRGSLESYIGKWALNKRVDRYIGMKKKTVLRDLIDYNLR
                     KTPIKSSSLKKAYEKGDGFAQALMNEYYCKFLAVGISQAVNMLNPDLVILGGGIMEAM
                     GVKLLPHVQSKLAMRCINPLPQIRLAELGDMAGPMGAAYLARKKQ"
BASE COUNT         1896 a         2042 c         1913 g         1793 t
ORIGIN      
        1 tgccttcttc cagtcgtggc cgccacctgt ggacgggccg tgcaagaatc cttcgcggcc
       61 tgcaatgaca gccagagcaa acgcctctgc ggtacgaacg ggatcgcgcg cgacggcaat
      121 cgccgtgttc acaagtgctg cgtgtgcccc catttccagt gcaagcgctg cgtgggaagg
      181 agcacccagt ccggcatcga caaccacagg aacgcgcgcc tgctcaatga tgatgcgaat
      241 gctttccgct gtgcgaattc cttgattgga tccaatcgga gaaccaagtg gcatcacagt
      301 gacgcagcct gcgtcctgaa gttttaaagc aagaatcgga tctgcattga tgtaaggcag
      361 taccttgaat ccttctttca caagaactgc ggcggccttc aacgtttcca ccggatccgg
      421 taacagatac actggatcag gtgtgacttc cagcttcaac cagtctccgc cgcccatttc
      481 ccgcgccaac cttgccaggc gaattgcttc ttccgcatcg cgcgcgccac ttgtattggg
      541 taaaaggaca attctgtccc gatcgagatg cgaaaccagg tcctgatctt ccagcttctg
      601 actcaaatca atgcgccgaa gtgcgaccgt gaccatctcc gttccagaag ctcggaccgc
      661 ctctgccata atggacggcg acgagaactt gcctgtgccc acgagcaaac gggaagcaaa
      721 ggcccgtccc gcaatcacaa gaggatccgg gtccctcgcc gacgcggact tcacaagttg
      781 atcgatagca tcgctcatca ttttcaggga aaaactcgac agcgcaggcg acaaatggat
      841 tttttttcta ttggacccga tcccttttga agctccggcc ttcgcgcgca acccgcactt
      901 tcaaacgatc gccactcatc tgatgcagaa agtgccaggg tatatgccca gggtgctcgg
      961 actcgtctat gaaactgcag tcgtgccgac tctcgatggc aacggggacc agctgcttct
     1021 tcacgttcac agatttcatg atcatgacca accggcggtc ctcctcgtgc atggacttga
     1081 aggctccgcc gattcgcact acatcgtgaa gatgacggac aaacttctcg cggctggatt
     1141 ccatgtagta cgtatgaatc tgcggagctg cggcgcagga cgcactctcg cgcggacagc
     1201 gtacaactgt gggctaacaa tcgatgtaga agccgcgctg aagttcatgc aggaacacgt
     1261 ttcgcgagcc atcgcactcg tcgggttcag cctcgggtcc aatctaacag taaaatacat
     1321 gggagaagat cgatcagaaa gagaccagca aatcatcgca cgtaaaggaa agccaattcg
     1381 cactcgcatc cgtgaccgaa ttgcggaagt cttttgcgcc atcagcattc cactcgatct
     1441 atatgcttca tgcgaatacc tggattctga caactgccgt atttatcgcg acgcgttcat
     1501 ggctgatttc aaaaagaaag tggagtcagg gaaatttgat cagattgccg gtgcgcgcga
     1561 agaaatcaag aatgtgaaga cctggtttga ttttgatcac tactatacgg caccttccac
     1621 tggttttcga agcgccgttg actactatgc ttcatactcc agcagaaact ttgtttctcg
     1681 catagacatt ccagggctca tgctccatgc gcgcgacgat ccgctcattc accctggcgc
     1741 ctgggaagaa acggactgga gtggttacaa acatctcaca gcagaaatca cggagcacgg
     1801 aggccatcta ggatggctgg gacagaagca tcctttcttc ccggaccgca gatggatgga
     1861 ctatcgcgtt ctgaagtatt tactggagtg gaaagattcc agaaagctta aaaccaaacg
     1921 ttattcgctt ttctccgggc cctgataaga atagtgagtg ggctgaaaac ttttctcaaa
     1981 aagcttagag cggaaatctt gaaagtgtcg agattgcgaa tcgtacatgg tttccgagcc
     2041 ctgaaagatc ccgaaatcaa agaccttccc cagcctttca tatgctgcag gagtcaaaac
     2101 tggtttccca tggtactcat cgacgagata gagaagtggc tcgccgctcg aaacctcctg
     2161 aaacggaatg gaggaaactg cggggaacgg gtatggctga tctctcctca tcttcacatt
     2221 gacgggagga cctgagcgct ggtatgtctt gctcttttcg gggcgaaaca gcacccagtt
     2281 tccggaggaa cgctctacgg ttccctcttc ttcatatgca ataaactctt cgttgtctac
     2341 actgacggct gcgtatagat gcaatttcgc ataacgtcca ctttcggaaa tctcaatttg
     2401 ttcgcgcgca tccgaatagg tggttctgtg cgcatacgga aaacgattct ttgcagcgcg
     2461 cacccatttg ccagcgggta gattcaatcc aaccgcgcga agaccaagtg cgcccccgtg
     2521 atcaggaaga ctgtggaccc aggaaggtct ggacgcgcag gaaaccataa caagcaacaa
     2581 gagagccgat ttaggcagcc gcatgagtgg agattcgcat acaccccggt ttttacaaga
     2641 aaaatgctgg aatcaggaga agggaactgg agtcaaagaa atgaccgcaa gaaccgggtg
     2701 gaaacggagg aagaaatcgg ataatctcaa atacatgaca caggcatcca cagactacca
     2761 gagaatagag aaggctctat gctacatcga ttccgttttc ccggctcaac cgccgctggc
     2821 tgaaatcgcc cggcacactg gccttagcga atttcatttt cagaagctct ttcaaagatg
     2881 ggtcgggatc agcccaaaac gctttctaca atttatcaca aaagaccaca gcttgagaat
     2941 cttgaaagaa tcgcgaagca tactcgatgc ggcactcgat gcaggattga gcggtccagg
     3001 aaggctgcat gatctgctcg tggagtgcga tgcgatgaca ccaggagaaa taaaatccgg
     3061 tggccgcaat ctattgattc gctacggaac cactacgagc cctttcggac tctgcatgct
     3121 tgccatcacc gatcgaggca tttgccgact ggaatttatt gaaaccagaa aagacgaaaa
     3181 gcgaatccag aatcaatgga aacaggattt tcctgaagcc acatttcgag aagacgtggt
     3241 tgcggcagac gaaatgaccc tgcagatatt cggaggcaaa aaagccgacg cactttctgt
     3301 tttgttgaat ggtactaact ttcaaatcca ggtgtggcag gcactcttac gaattccttc
     3361 cggctccgta ctttcctacg aagatgtcgc gcaaatgatc ggccgacctg aggcaacgcg
     3421 cgcggtggca agcgccatcg cacacaaccg aattgcattt ctcattccgt gccatcgcgt
     3481 gatccgcaaa atgggagtca tccacaagta tcactacgga accaccagaa aaaaggcaat
     3541 gctcggctgg gaggcaggaa tgaagagacc gtctctcaga aatactcagg aggaagaatg
     3601 agggaaccaa atggaaattg acgcgcgacg cgctgcacgg tagcaggcaa tatgccggtc
     3661 gcatacctgg gtaagaaggg aagctttgct tccctggtcg cacgacaaag atatccggac
     3721 gcagagactg tctcacagcc aacgattgga gatctggtta cctacgtgaa agaaggagcg
     3781 gaccgaatgg gtgttgtccc aatcgaaaat agctccggtg gaatcatttt tgatactgtg
     3841 gacgcttttg tggatgattc attcaatctt ttcatcctgg aagaaacgcg catccgcgtg
     3901 cgatacgctt tactgggaaa atccgggcaa ccaattcaga ccattctctc ccactatgca
     3961 gctctgcgcc actgcgcagc gtggattcgc tctacatatc cagcagtcag gacacaacca
     4021 gttgccagca cgtcagaggc cgccgcgatg gccgccgcgg gatctcagct tgctgcgatt
     4081 tcaacgcgcg aatccgcaaa cgagtacggt ctggacgttc tgcattttcc aatcatggat
     4141 agcgttccca acattacgca attctttacg attgggcatg cgccagtaaa ctactcgggg
     4201 tccggaggaa acacaagcct tgtggccact ctggacaatt caccaggaag cctttttcga
     4261 ttcctcaaac cctttcatga cctgggagta aacctgaata gaattcaatc gcggacgatt
     4321 ccgggagact caagcgcgtg caaattcttt gtagggctcg agggagtgga atctgaaacg
     4381 aatgtgggtc gcgccataga actggctcgc cctctcacgg ttgcgctgca actgcttgga
     4441 tcctatccga gattgatgga cccttacgaa tcttgacggt aggtcactct gctttttgca
     4501 gaaaatcaga atcgtgcaga tcttcttcag gcatagtatc cgagtaatcc gccggatctt
     4561 cgcttccgtc tgaaatcatg gcttgagttg aatccatcga agaagcggga ttggattttc
     4621 cctgaactgt ttctgttcga tcatccggcg cagcggcatt acctttgcct tttccgcgga
     4681 attgaaacac agagcgcccg agcttgatct catcgccatc atgcaagccg cgagggcgaa
     4741 gtaatttctt tccattcaag aaaacgctcg aaccacccat tagatcgtat aacacataac
     4801 ggctgtccac acgctttacc tttgcgtgaa ctttagccgc tcctgaatcc tctacgcgaa
     4861 tgctggccca tctgcccgaa ccaagggccg tgttttcaag gaagagatca tactctcgcg
     4921 acattccatc tttcatgcga atcagagaag cagcagcata acgaggtgct ccgcggagtg
     4981 cctcctgcaa agatcgatat gcgttctcgc gcaaatactg aacagcagga tgaggcatgt
     5041 cctctgctgg aattccttct acaatatcta ctttctctgt ttcgccttcg ggaagcttca
     5101 catgcgccgt tggcgaatcg gaagcagaaa caactgccgg gttcgtggct tttgccgcgt
     5161 catgcttttg tttcagcaca aggatcagag ctacaacagc ggccacgaac aagagaaaag
     5221 aaagcactcc gagcaataca ttgccccatg gaatggaatt gtaccacggt ccctctgaat
     5281 gtgggatcga tacggatgga gcgttgtaag aagccgtatc ttcatcgatg gtggttcctc
     5341 tttttacaga tagaacaatg ttgcgcggcc ctgcaccctc cggtgctttg taagaaaggc
     5401 gaaacgtttc atctttgaga agtcgaatgg acctggaaag atttcgcatt gtgtcaggcg
     5461 taagcatggt aatgagctta ccgcccgtgc gcatggatag tctctggaag acgcgttgat
     5521 tgcgcggatg tccgtataac acagtgtaga cgggaactcg cagttttgtt cccagttcag
     5581 ccagctcaag acaatcctgt tcgtttagat aactcgcttc atcccggcca tctgtaaaca
     5641 gtaagacggc cgcttttgcg atttgcttgc ttgagctctc cgtgttttga ttgcgaagga
     5701 ttccttctcg cgccgcgttg ataccgctgt gaagcgcgtc aaaaatgcga gtcgttctgc
     5761 cgactcgttt catttgcgct atttgttcgg aaatcgtttt gccgccagga acaaatccgc
     5821 tgatgtttat acttccgcca ttgatttgaa agagggcagt gcgatcgctt tctgtgagac
     5881 tggataagaa aacgccagca ccgttctgag cgcgacgata gtccgccgcc ggaatggatc
     5941 cggtcgcatc aaatacgact gccagaacca gacgatccgc ttctttcctg gaggcagtga
     6001 tgctcggcgt gatgggcgcg ccatcgcgca gagtgaattg aggtgttccc tctccttcgc
     6061 ccagttccgc cagtatttct acgtttggaa aatgagatga atcgacctcg agcacgcgca
     6121 gagccgggcc agcatgaaga acaaatggaa tgagccatag caggcagaag cgtgccattg
     6181 ctttgattat cggccgatct tacggactgc cgatgattac atttccttcc gtttccttca
     6241 tcttctcgag gatttgtctg ccttcagggc tcgaaaatcg gtagtattgc caggcgtgat
     6301 gtccgcccag accaagaaat ccaagcgcca tgatccaggc agccagcctg cgagcgcctg
     6361 tcatttcccc gagtaccccc cggtgcaaaa agccaagggc aatcagaacc agatagatgc
     6421 ctatcccgga gtatattata gaataaagta tatctcgtat cattgttttt tccgtgccag
     6481 gtacgcggcg cccatgggac ctgccatgtc tcccagttct gctaggcgaa tctgtggaag
     6541 tggattgatg cagcgcatgg caagcttgct ttgaacatgg gggagaagct tcactcccat
     6601 ggcctccatg attccgcctc ccaggatcac aaggtccgga ttcagcatgt tgactgcctg
     6661 cgaaatcccg actgccagga acttgcaata atactcgttc atgagtgcct gcgcgaaacc
     6721 gtcccccttc tcatacgcct ttttcaaact ggacgatttg atcggagtct ttcgcaaatt
     6781 gtagtcaatc aggtcgcgaa ggactgtttt ctttttcatt ccaatgtatc gatccactct
     6841 cttattgaga gcccattttc caatgtaaga ctccagactt cctctcaaac cactgtccgt
     6901 ctttctgcca ccatctctga caatgatgtg ccccacttca ccggctcctc catcgcgacc
     6961 aagaaccagt tcgccattta tggcaatggc tccgccaatt cccgtaccgc agaaaaacgc
     7021 aatggcaatc gacggtttgt gttgcatgcg cgccagttct cccataagac ctgcgttcac
     7081 atcgttcaca agcacggaac gaatgtccct ctgcacaaga agtccgagat cctgcaaagg
     7141 gtgtctattt tcccagttca agttgggagc aacacgcacc aggtctgtgt ccttggaaac
     7201 cacgccggga acgcccaggc cgattgtctc aattcgaatt cctttgcttt cgcaactggc
     7261 aatcagcgcg tcgatctggc cgcgcaaacg accaatgacg tgattgtagc cttcttttgc
     7321 ttccgtgtcg attttgcttt gcgtaaccgg gtggtaggag tcgtcaaaaa cgactgagaa
     7381 aatgtttgaa ccgcccagat ctattccaag gtgccccgat tgcataaatc ggagtatgaa
     7441 gggaggtcca cactgtcaat cgattgatga cagttcaatc tccccggcga taatcctggc
     7501 gctggcggat gcttctgcca agtgtgactc gatccgcata ctcaatggca gcacccgttg
     7561 gaacgccatg agagattcga gtgatgttca aattgcgacc ggtgagcgcg ttggcgatgt
     7621 agtctgcagt tacgtctcct tcca
//
LOCUS       DAOQJL010000131         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5229_length_7641_cov_4.188505, whole genome shotgun sequence.
ACCESSION   DAOQJL010000131 DAOQJL010000000
VERSION     DAOQJL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5229_length_7641_cov_4.188505"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLG78_07625"
     CDS             <1..303
                     /locus_tag="PLG78_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNE23020.1"
                     /translation="GVLETLPLAEISHFEAADKQVIIHQNHGRKRTCTRKLGDVEKVL
                     PPRFIRVHRSFIADTGQIKTLQAAGGGRYDLTMQSGVTIPVSRSAYASLKKNLMQS"
     gene            complement(443..649)
                     /locus_tag="PLG78_07630"
     CDS             complement(443..649)
                     /locus_tag="PLG78_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNE23021.1"
                     /translation="MKFELKGHEYIQLNQLLKFLGLVETGGEANQCIVDGQVLFNGSV
                     ELQKRKKVRRGDTVEFQSKKIEII"
     gene            complement(692..2425)
                     /locus_tag="PLG78_07635"
     CDS             complement(692..2425)
                     /locus_tag="PLG78_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE23022.1"
                     /translation="MIQSNYFADNPDLQDHVERFVDWQEMIGLYEDGFRDAQEYKATG
                     NERLSMAPSTNEEALAYYHQMLDAYGDMCGNELSQLAAPMDRIGLKFENGKVTHPAEI
                     EKLFEKFDDAGLRPIAFQKKYGGLGVPNVLKALCQEMSYRADGALSITCGSVNLAAIL
                     EAYASKEMRDEWIPKLIHERYSVTMGLSEADFGSDLPSVRTRAEKIDGKWYLTGTKRF
                     QTMACGLNKYPAVLLALARTGAPDSGARGLSFFLVDSKDFEVTGIEKKLGIKASPTCE
                     VAFEKAPGLLIGEEGHGLSRYVIGMLNGARLSVASQGTGIATAAWYEARKYASERIQF
                     GKPIDQIPAVAKMLRRMERESKAMRCLMVEAASAVDRYHWKALRAIDSDEKTKKDDFG
                     RKWEKLASTITPMAKYYISEMANSVAYDAIQVFGGAGFIEEYDVARLYRDARITNIYD
                     GTTQIQVNAAIGGVVSGMAETGGLRAHLQEMLDLVAKGESSRAAILKELFAEFESLVS
                     HFKTLEGAKKDSFAFEVVETATRTLNSLLLAKTSLKLTGTAREERFQLTHEYAVESLG
                     TAQASKLRMTR"
     gene            2594..2989
                     /locus_tag="PLG78_07640"
     CDS             2594..2989
                     /locus_tag="PLG78_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23023.1"
                     /translation="MRLLSIFHYVNAGLTATGLLFLAGHYAIMHTVFTNPALTKSPKG
                     DGPPLEFFKVFLWFYVLGAVFLTVTATLNVLSAYFIQKRINRVFSIIVAGLNCVLSMQ
                     CPIGIGLGVFTFVVLFRESVRELYDVAAR"
     gene            2986..3891
                     /locus_tag="PLG78_07645"
     CDS             2986..3891
                     /locus_tag="PLG78_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE23024.1"
                     /translation="MIQSPRLIPGRTNAIALGLLFPTLLLLLMFGPGTHGITFAVATV
                     AFSILGFTAYCFLHEAEHELLFESRFLNDGAGVMLGLFFPAPFHLLRQGHLGHHARNR
                     TDTEAFDLYHPNRPREKWMRRFQLYGTLTGIWYLVVVLSNVLAFTWPLPLLFRRTDRS
                     ARALLSSFNPEKIGIVRLEALLALLFHGTLLWLSDSPGFYLLMYFFSGLLWSSQQYLH
                     HYGTERHPERGARNVRTWKFLDLLWLNHNLHLTHHLKPSLPWIHLRLNGPPATTTMMS
                     AYWQMWRGPVADASTVMDATEGRVV"
     gene            3906..6086
                     /locus_tag="PLG78_07650"
     CDS             3906..6086
                     /locus_tag="PLG78_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE23025.1"
                     /translation="MLRRHFRLKAKVALLLAVGIPGPYFLLQYFPILQATPAPSIFLD
                     GKIPFIPETMWIYQTLYPCLLLVGFSLFRRHDVIRYSWTLLLASVIADFVFLIHPTFV
                     PRPDFAQGLYGIWTQIERPMNAIPSLHAAFIAISWNYAGTKSRSSMRPAQVLFSVWMI
                     VILISTIATSQHRWLDLAVGLLLGQFCSLLIRRPHPARSVLIVTGSLLEPSGSIAGAL
                     LRMLAQRSSEKHGMFSLKIKAVIGEIFVYGILERLGRQRYAAYISEYFKGPPSPPDLT
                     HSLLETMLRKEGIPTDHITVAQVFANPFRTARLLRKHSCIFLSSTLLHDQSELTTVAS
                     ALKRPWNRVVVGGALTGSLPTTFGAAHIDMLAPGYGEILVPALAPWIQSGFRNHAFES
                     LRVRRIQSMIRVEAFPPSSRDLDFLPSPDFHGQPETIYYESVRGCPYRCSFCNYPYLF
                     SDANFRFKSARKIYDDWMQYAGSGVRTIISLDSLFTIPRKRLDQLCNSLIRAGSPVRW
                     ICYVRADDIDLERAQMMREAGAFQVHIGIESGDDAILANMNKKTSAEVNARAIHNLRR
                     AGITSIATLIVGFPGETEQSIQTTLNFLQQNPPDFHFLAAFSTRVPGVPVLSEESKSR
                     FGLVVNTELATMSPYWSHATMDCAKAGVFLRNATKYLIENRISLDAALFHKGLSGYDP
                     AHRAELLDFQEAAIRRYTTLRRFMDAVHQKIDHALARDMAAFFQ"
     gene            6093..6905
                     /locus_tag="PLG78_07655"
     CDS             6093..6905
                     /locus_tag="PLG78_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23026.1"
                     /translation="MSADLYEHVDKECFRLYVRFIRRHIRYAPIVIALYGYSLFQWKK
                     ARPLRAGFALAQAVDDMLDGDWQIQSDPIELTRLCVRDPSAVHDDGLKQICVYLIPWM
                     KEHGLLDSLSSLFETLILDCKRRSTREQWPGEKLKNHHFRTFELSAKIALGLCELELS
                     PADARGVIESLAWVSPMRDLREDLEKGINNIPSEVVDTSRMTAKEALSNRRVIEWMKT
                     EYQSALLSLDRSNLELKRIQDKKGAVYFRIFHRLVKSYARKYPRKYPEILSC"
     gene            6899..>7641
                     /locus_tag="PLG78_07660"
     CDS             6899..>7641
                     /locus_tag="PLG78_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23027.1"
                     /translation="MLTRPFVQISNGLKWMKDPCSLLDLMAARHGLTFRLDLPILGNC
                     LITGDPDLLAAIKNHPNLEAGKGIRALRRILGDGSLITLDGEAHATRREIIAKHFRAD
                     QSQAVDALTVDVMRSLYEDLPDGDFSAYSLFHEASLLVILRFLFEDLTLKELEDLRSD
                     TLLFLSAFSSAVVLFVRPLQIDHILFPWGRALNHRRNLEKSILKFSRRQNRASQIFAE
                     MEEAGSTNETACAEILTLLMFGHDTAAAA"
BASE COUNT         1768 a         2070 c         1800 g         2003 t
ORIGIN      
        1 ggcgtactcg aaacactacc tcttgcggaa atctcacact ttgaagcagc agacaagcaa
       61 gtgatcattc atcaaaacca cggtcgcaag cgaacgtgca cgcgaaagtt aggcgacgtt
      121 gagaaagttc ttccgccgcg tttcatacgc gtccaccgct ctttcattgc agacaccggc
      181 cagatcaaaa cgctacaggc cgccggcggc ggacgttatg atctgaccat gcaaagcggc
      241 gttacaattc cagtaagccg ctctgcctat gcgtcgttaa aaaagaatct gatgcagtcg
      301 tgatctaagc gaaacccgtc gtagtcgggt aatccgcaac atcaccttta cacatcctgg
      361 agggtgtccc gtttccgagc cgtggccccc caccgacatc ggtcgcggtc agggtgtaat
      421 tattccgctc tgcgcgcagc ttctaaataa tctcaatctt cttagattga aattccaccg
      481 tgtctccacg gcgcactttc tttcttttct gaagttctac gctcccgttg aacaagacct
      541 gtccgtccac aatgcactgg ttggcctccc ctccagtttc cacaagtccc agaaacttca
      601 agagctggtt gagctggatg tattcatgac ctttgagttc gaatttcata ctcgaagcag
      661 tgccagaatc aagctcattc gccgcgacaa ctcatctggt catacgcagt ttggatgcct
      721 gcgccgtacc cagactctcg accgcatact catgtgttag ctgaaatctt tcttcacggg
      781 ccgtcccggt aagcttcagt gatgtctttg cgagcaaaag tgaattgaga gtgcgtgtag
      841 cagtttccac tacttcaaag gcaaaactat ctttttttgc gccttcgagg gttttgaaat
      901 ggcttaccag tgattcgaat tcagcaaaca attctttcaa aatcgcagcg cgggaagatt
      961 cccctttcgc tactagatcc agcatttcct ggagatgcgc ccgcaggccg cctgtttccg
     1021 ccattccact caccacgccg ccaatcgccg cgtttacttg aatctgcgtg gtgccgtcgt
     1081 aaatattcgt gatcctggcg tctctgtaca gacgcgccac atcatattct tcgatgaatc
     1141 cagcaccgcc aaatacctga atggcatcat acgcaacgga gtttgccatc tcagaaatgt
     1201 agtactttgc catgggagtg atagtgctcg cgagtttttc ccacttgcgt ccaaaatcgt
     1261 ctttctttgt cttctcatct gaatcaatcg cgcgcaaagc tttccagtgg tagcgatcca
     1321 ctgcactggc tgcttctacc atcaggcatc gcatggcttt ggattcgcgc tccattcttc
     1381 gcagcatttt cgcgactgca ggaatctgat cgattggttt tccaaactga attcgctcgc
     1441 ttgcatattt gcgcgcttcg taccaggcgg ccgttgcaat gcctgtcccc tgcgaagcta
     1501 cactcaggcg cgctccattg agcatgccta tcacataacg agaaagacca tgtccttctt
     1561 cgccgatgag caggcctggc gctttttcaa aagcaacttc gcatgtaggg gatgctttga
     1621 ttcccagttt cttttcaatt ccggttactt caaaatcttt actatccaca aggaagaaag
     1681 acaggccacg agcaccgcta tcgggcgcac cggtgcgagc aagagcaagt agaaccgcgg
     1741 ggtatttatt gagtccacag gccatagtct gaaaacgctt ggttccggtc agataccatt
     1801 tcccgtctat tttttcagcg cgcgtacgca ctgacggtaa gtcggaccca aagtctgctt
     1861 cactgaggcc catggtgacc gaataacgtt cgtgaatcaa tttcggaatc cattcgtcgc
     1921 gcatttcctt gctcgcatat gcttctagaa tcgctgccag attcacagat ccgcatgtaa
     1981 tgctgagcgc tccatccgca cgataactca tttcctgaca gagtgcctta aggacgttag
     2041 gaacacccag gcccccgtat tttttctgaa acgcgatggg ccgcagaccc gcatcatcaa
     2101 atttctcgaa tagtttttca atctccgcag gatgagtgac cttcccgttt tcgaatttca
     2161 gaccaatgcg atccatcgga gccgccagct gcgagagctc gttgccacac atatcgccat
     2221 aagcgtcgag catctgatgg tagtaagcaa gagcctcttc gttcgtggac ggtgccatac
     2281 tcagacgttc gtttccggtt gccttgtatt cctgagcgtc cctgaacccg tcttcgtaca
     2341 aaccgatcat ttcctgccag tctacgaagc gttctacatg gtcctgtaaa tcaggattgt
     2401 ctgcgaaata attgctttga atcattcggc aaaactggcg aagattgcgc ttgtgagtat
     2461 cctttttttc tggatccagt ccgggtaaaa agcacttgca gacgaagcgc tcacagggcg
     2521 aaatctgctg gtggatcaaa caaacgagct cccacctatt ttgaaagatc aacgaaaaac
     2581 ggatgtcgaa catttgcggc ttctttccat ctttcactac gtaaacgctg gactgacggc
     2641 caccgggttg ttgttcctcg cgggacacta cgcaatcatg catactgttt tcacaaatcc
     2701 tgctttgacg aagagtccaa aaggagatgg accaccatta gaattcttta aggttttctt
     2761 gtggttctat gttctagggg cagtattctt aactgtgact gcaactctca atgtcctttc
     2821 cgcatacttc attcagaaaa gaatcaatcg tgttttttct atcattgtgg caggactcaa
     2881 ctgcgttctc tcgatgcaat gcccgattgg gattggcctt ggggttttta cgttcgttgt
     2941 tttgttccgc gaatctgttc gcgagctata tgatgtggcg gccagatgat ccaatctccg
     3001 cggctgattc ctggccgcac gaatgcaatc gcgctggggc tcctattccc gacattgctg
     3061 ctccttttga tgtttgggcc gggcacacac ggaatcacat ttgccgtcgc cacagtcgct
     3121 ttttccatcc tcggtttcac ggcgtattgc ttcctgcatg aagcagaaca cgaacttctt
     3181 ttcgaatccc gcttcctcaa cgatggcgca ggagtgatgc tcggactttt ttttcccgcg
     3241 ccgtttcatc ttctcagaca gggacatctg ggtcaccatg cgcgcaatcg tacggacacg
     3301 gaagcgtttg acctgtatca tccaaataga cctcgagaaa aatggatgcg aagatttcag
     3361 ctttatggta cgctcactgg aatctggtat ctggtggtag tcctgtcaaa tgtactcgca
     3421 tttacctggc ccttgccgct tctatttcga cgaaccgatc gcagtgccag ggctctgttg
     3481 tcttcattca acccggaaaa gattggaatc gttcgcctgg aagccttgct tgcgctcctg
     3541 ttccatggca ccttactctg gttaagcgat tcccccggtt tctatttgct gatgtatttc
     3601 ttctccggtc ttttgtggtc ttcacagcaa taccttcatc actacggcac tgagcgtcat
     3661 ccggaacgcg gcgccaggaa tgtgcggaca tggaagtttt tggatctgct ctggttgaat
     3721 cacaatctgc atctcacgca tcacctgaaa ccgtccttgc cctggattca cctgagactc
     3781 aacggacctc ccgcaaccac gactatgatg agtgcctact ggcaaatgtg gcgaggacct
     3841 gtagcggacg cgagcaccgt catggatgct actgaaggta gagtcgtttg agacggatcg
     3901 tgcgaatgtt acgacggcat tttcgtctca aagcaaaagt ggccttgctc ctggctgttg
     3961 gaattccagg gccctatttt ctactacaat actttcccat tctgcaggcc acacccgcgc
     4021 cttctatttt cctggatggc aagattccgt tcattccgga aaccatgtgg atctaccaga
     4081 ccctttatcc atgcttactg ctcgtcgggt tttcgctctt tagaagacat gatgtgattc
     4141 gctattcctg gactcttctt ctggcaagcg tcatcgcgga ctttgtgttt ctcattcatc
     4201 ccacgtttgt cccgcgaccg gactttgcac agggactcta tggaatctgg acgcagatag
     4261 aaagaccgat gaacgccatt ccgtctcttc acgccgcctt catcgcaatt tcgtggaatt
     4321 acgcagggac caaatccagg agttcgatgc gaccggccca agtgcttttt tccgtctgga
     4381 tgattgtgat cctgatcagt acgattgcga cttctcagca ccgatggttg gatcttgcag
     4441 tcggtctttt gctgggacaa ttctgctctc ttctgatccg tcggccccac ccggcgcggt
     4501 ctgtactgat tgtaaccggg agcctgcttg aaccgtccgg ttccattgca ggcgctcttc
     4561 tcagaatgct tgcacaacga tcttcggaaa agcacggaat gttttcactc aagatcaaag
     4621 cagtcattgg ggagattttt gtctacggga ttctggagcg actcggcagg caacgttatg
     4681 cggcatacat atcagaatat tttaaaggac cgccatctcc tcctgacttg acccactcac
     4741 tcctggaaac catgctgcgc aaggagggca tccccacaga ccatataacg gtcgcgcagg
     4801 tgttcgcaaa tccatttcga acggcccggc ttctccgaaa gcattcctgt atatttcttt
     4861 catccacgct tctgcacgac cagagtgagt taacaacagt agcttccgca ctcaagcgcc
     4921 cctggaatcg agtcgtggtg gggggcgctt tgacaggcag cctgcctacc accttcggtg
     4981 ccgctcacat agatatgctt gcccctgggt atggagagat tctagttccg gcccttgcac
     5041 cgtggatcca atctggattc aggaatcatg cattcgaatc gctcagagtg aggcgcattc
     5101 aatccatgat acgagtagag gcatttccac cttcctcgcg agacctggac ttcttgcctt
     5161 cgcctgactt tcatggtcaa ccagaaacaa tctactacga atcagtacgg gggtgtcctt
     5221 atcgctgcag cttctgcaac tacccttacc tgtttagcga cgcgaatttc agattcaagt
     5281 cagcaagaaa gatctatgac gactggatgc aatatgccgg gtcaggagtg cgtacgatca
     5341 tcagccttga ctcccttttt acgatcccgc gaaaacgact cgatcaacta tgcaactctc
     5401 taattcgggc cggctctccc gttcgatgga tctgctacgt tcgcgcggat gacatcgatc
     5461 tggaaagagc gcaaatgatg cgagaagcag gtgcgttcca ggtccatata ggaattgaat
     5521 ccggtgacga tgcgattcta gccaacatga acaaaaaaac tagtgcagaa gtaaatgcgc
     5581 gcgccatcca caatctcaga cgcgccggca tcacaagcat tgccacgttg atcgttggtt
     5641 tccccggaga aacggaacaa tccattcaga cgactctaaa tttcctgcaa cagaatcctc
     5701 cggactttca ttttctcgca gcatttagca cgcgggtacc gggcgttcct gttctttctg
     5761 aggagagcaa aagtcgattc ggcctggtgg tcaacacgga gcttgccacc atgtcaccgt
     5821 actggtctca tgcaacgatg gactgcgcga aggccggagt gtttctgcga aacgcgacca
     5881 aatacctgat cgaaaatcga atctccctcg atgctgcctt gtttcacaag ggtctttctg
     5941 gctacgaccc agcgcacagg gcggagttgc tggattttca ggaagcagcg atcagacgtt
     6001 atacgacgtt gcgtcgcttc atggatgcag tgcaccagaa gatagaccat gctcttgctc
     6061 gagatatggc tgcttttttt cagtaacgga ccatgagcgc agatctttac gaacatgtgg
     6121 acaaagaatg ctttcgcctt tacgtgcgat tcatccgaag gcatatccgt tatgctccga
     6181 ttgtcatcgc attgtatgga tattccctct ttcagtggaa gaaagctcgc cctcttcgcg
     6241 caggattcgc attggcccag gcagtggacg acatgttaga cggggactgg caaatccaat
     6301 cagacccgat cgaactcaca cgtttgtgcg tccgcgatcc ttccgccgta cacgatgatg
     6361 ggctaaaaca aatttgcgtc tatttgattc cctggatgaa agaacatggt ctgctggatt
     6421 ccctttccag tcttttcgaa acgctcattc ttgactgcaa gagaagatcc acccgcgaac
     6481 aatggcctgg cgaaaaatta aagaaccatc actttcgcac atttgaactt tccgcgaaga
     6541 ttgcacttgg actctgtgag ctcgaacttt caccggcgga cgcgcgtggc gtcattgaaa
     6601 gcctggcctg ggtatcaccc atgcgcgatc tgcgcgaaga cttagaaaaa ggaatcaata
     6661 atattcccag cgaagtagtg gatacttcgc gtatgacagc aaaagaagct ctttccaaca
     6721 ggagagtgat cgaatggatg aagacggaat accagagtgc actcctgtcg ctggatcgat
     6781 cgaatctgga gctcaaaaga atacaggaca aaaaaggtgc cgtgtacttt agaatcttcc
     6841 atcgactggt gaaaagctac gccaggaaat atccgcgtaa gtatccggag atcctcagtt
     6901 gctgactcgc cctttcgttc aaatctccaa tggcctgaaa tggatgaagg atccgtgctc
     6961 gctcctggac cttatggctg ccagacatgg actcaccttc agactggacc ttcccattct
     7021 gggcaattgc ctcataacgg gagatccgga tcttctcgcc gccatcaaga atcatcccaa
     7081 cctcgaggcc ggaaagggaa tccgggcgct ccggagaatt ctcggggacg ggtctctgat
     7141 cacactggac ggagaagccc acgcaaccag acgcgaaatc attgcgaaac attttcgtgc
     7201 agatcaatcc caggctgtgg acgcgctcac agtggacgtt atgcgctctt tatatgaaga
     7261 tttgccagac ggagacttca gtgcctattc tttgtttcat gaagcatcct tgctcgtcat
     7321 acttcgcttt ttgttcgagg atctaacctt aaaagaattg gaggatctga gaagcgatac
     7381 tctgcttttt ttgtctgctt ttagcagcgc cgtggtttta ttcgttcgtc ctcttcaaat
     7441 cgaccatatt ctttttccct ggggaagggc attgaaccac aggcgaaatc tggaaaagag
     7501 catcctgaaa ttctcgcgaa ggcaaaaccg ggcgtcccag atctttgctg aaatggaaga
     7561 ggctggatcc acaaatgaaa ccgcctgcgc tgaaattctc actctgctca tgttcggcca
     7621 cgacaccgcg gcagctgcga t
//
LOCUS       DAOQJL010000132         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5240_length_7634_cov_4.581475, whole genome shotgun sequence.
ACCESSION   DAOQJL010000132 DAOQJL010000000
VERSION     DAOQJL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5240_length_7634_cov_4.581475"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PLG78_07665"
     CDS             <1..1593
                     /locus_tag="PLG78_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNE23028.1"
                     /translation="PNLLKRHAREDQLHDQEVEERWHKRVNVPRIARILLAIMVLSVI
                     ASSFGFTRVKWDNDSRNMIDYNIASERLQEEIALRYELTGSPAVIATKSLDEARALFE
                     HFEPPTPAVADIVAQTISIWAYVPPYQQQVQNSELMQKFRADSSQIKPGILPPQYRHF
                     YPIYQTIIGQKPFGVKDLPKDTISQFESKPESKEKGWLTFFYPVASKLYTVDGLNQME
                     RQTGKISVPIVGRQSLKSLAYFAPKLEVALRKKAGGDAAKKVSLDGMEFTEREVSLAL
                     FAANSGDQKFLKSTGLSDLAIETILKHRPFANVEALQNEKLSVQGTGSVLIVARFANL
                     VLEESKTIILMSLSLVVALLYLSFRDLRSTLIALVPMIAGLVLMIGLMAIFNVKVNYF
                     NVAVLPIVIGYGINNGFFLFYRFMESGSIFQTMFHTGVAGLGSALTSLAGWGAIASAT
                     HPGLRSMGYLAFLGLSAMTIAMLVFLPALLALLNSRYPALIERLRLRHGGTAKADPSE
                     IEVPVAVTSAAEGSTKRAKKGK"
     gene            complement(1600..2286)
                     /locus_tag="PLG78_07670"
     CDS             complement(1600..2286)
                     /locus_tag="PLG78_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNE23029.1"
                     /translation="MKVQKKRIAELESFVVEGKDKGPVVVLCHGFGANAQDLLPLAEA
                     LSAPAGTTWIFPDAPIEFPIGPGRMGRAWFPLVAEEIARLAATGKHLNFYEVVPPGLE
                     ASRKKLEALIHETGRPANLITLGGFSQGSMLAVDTYLHASETMAGLVIMSGNLICRDQ
                     WTRLAPARRGSQFIQSHGLMDPVLPYENAKRLETVLREGGLSGEFISFSGGHEIPQGV
                     IKKVSLYLTR"
     gene            complement(2283..2819)
                     /locus_tag="PLG78_07675"
     CDS             complement(2283..2819)
                     /locus_tag="PLG78_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE23030.1"
                     /translation="MMPEIFTSRIKLRAFRESDLDEFAAMNADERVMKYFPATLTRQE
                     TIELMRRIDDHEKKHGFTLFALEDISTGKFFGFTGAAVPAFDAHFTPCVEIGWRLAFD
                     FQGKGLATEAALATVNYAFAHLGVSELVSFTFKGNLPSRRVMEKIGMAYHPEEDFLHP
                     QLAREHHLAPHVLYRRKR"
     gene            complement(2829..3263)
                     /locus_tag="PLG78_07680"
     CDS             complement(2829..3263)
                     /locus_tag="PLG78_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23031.1"
                     /translation="MSFAQDNKSENDRLRAIMARLTDHHWSLDLGGDWTVGTLLCHLA
                     YWDRMTSVRIKKWMGDGTLTAVPDADNIECINNSVRFLSRGVGRVEGIRTVQEAADEI
                     DSIALSLSPDQLNFLEANGRDRWFRRSLHRQHHLARIEKALL"
     gene            complement(3344..4207)
                     /locus_tag="PLG78_07685"
     CDS             complement(3344..4207)
                     /locus_tag="PLG78_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23032.1"
                     /translation="MIRTTLLVLTFAVCLGLHAEETSPIQLDWQTFRIFSTGSDDRHL
                     LVFQKKKDPGPLLRLQAWDYGHQRIMNVAPMRGTSGELLYAQTFDRGESGGSITLTFF
                     KASGDAVQPLPFEIARSGYNAPEVQSGSGPSRILIPCEAFYGKRVGPFSLTGVYVSGH
                     GLSMIPRIAVMDQAQGKVNLKDVTFSTGVRSVLNGFLTQSEKALGEQSGKTWEGAEGL
                     QLAAGLATYYYFKIKLGEEADALAKITRAGIKILDSDQEKSSPAIHAADWLKTSRADI
                     VRFGDAEKLAE"
     gene            complement(4204..4635)
                     /locus_tag="PLG78_07690"
     CDS             complement(4204..4635)
                     /locus_tag="PLG78_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23033.1"
                     /translation="MSKETLSFISRTAQDDIFEVVFASYLKKDFISRALTLIHDSKLI
                     MRSLRFHSNDGGIAVWENGKGIISANSWEDEDAGIDPDSLMRFRFPLLNGAIAPDLAR
                     ETIIHDVISHLPNGELASILLKISKTKKWKEPLPSSYLEIL"
     gene            complement(4691..5299)
                     /locus_tag="PLG78_07695"
     CDS             complement(4691..5299)
                     /locus_tag="PLG78_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23034.1"
                     /translation="MIDLQNIQFKCTEPVDPSMEARLPEDLRTVLHKINGFTAFRGGL
                     HVRGACVAPSWHSLETIWDGKFSLRRTYELLGEKDIPFAEDCMGDQFILRDGMVYRLT
                     LETAFLDPLGLSLDGFFASANEDPVEFLQMHPLLRLEKEGKKLEPGQLIHAFPPYCTK
                     ESAQGVSLKAVEANAQIDFELDFFRQIRDIEDGGQIRIRVID"
     gene            complement(5296..6195)
                     /locus_tag="PLG78_07700"
     CDS             complement(5296..6195)
                     /locus_tag="PLG78_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNE23035.1"
                     /translation="MDSNSIYLQETIRVFRQYKELADKAIAQLDKDEFYFKDFGPRSP
                     SIASIAKHVSGNLLSRWTDFLTTDGEKENRNRDGEFVIQSGETRAVLTAFWEKGWAAL
                     LDTLSSLGPADVSRTVTIRGEPHSVPLAIQRSLAHTSYHVGQILYLARLAKEGEWKWL
                     TIPPGASAQFNQAMAGQFPKKSPRTIFHIISRDDWMAAGLAGSVRPSSLETEGFVHCS
                     TADQVAETANLFFSEAHDLLLLEIEEDRLTADIRFEAPSDAKSSNALFPHVYGPINLD
                     AVTAVHTLHPDSRGRFQFPTGSA"
     gene            complement(6232..6687)
                     /locus_tag="PLG78_07705"
     CDS             complement(6232..6687)
                     /locus_tag="PLG78_07705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE23036.1"
                     /translation="MEFEIRKARSLDEDEVYGLVRNFAVSFQPDRVTFSNSFEKLIRE
                     ESARVLVAVSQKTIVGYLLGFTHLTFFANGSVGWIEEIMVNERLRRQGIARALTVDFE
                     QWVTSRGGKLVALATRRASDFYRSIGYEESAIYFRKKLTTPAVGQTGFD"
     gene            complement(6695..7336)
                     /locus_tag="PLG78_07710"
     CDS             complement(6695..7336)
                     /locus_tag="PLG78_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE23037.1"
                     /translation="MQKLVVRCFSISVDGFGAGPEQSLENPLGIGGRDVHSWFFPTRT
                     FQQMFGESKGETGVDYDFAARGFENIGANILGRNMFGPIRGPWPNDEWKGWWGPNPPY
                     HSPVFVLTHHARQSIPMEGGNTFHFVTDGIESALKQAFAAANGKDVRLGGGVSLVRQY
                     LNKGLIDELHVAISPTILGSGERLFEGVDLVALGYKITEHVTTKNATHIVLTK"
     gene            complement(7339..>7634)
                     /locus_tag="PLG78_07715"
     CDS             complement(7339..>7634)
                     /locus_tag="PLG78_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNE23038.1"
                     /translation="CYDKEALASRKEHKPKDSAVNMANAMGIELLTEEEYRQLQELGE
                     FDTKTSSWVKTPADVRKLGGAVFCDRRYGRVFLYHNGAESYYAVRGFRGSLRI"
BASE COUNT         1755 a         1963 c         1916 g         2000 t
ORIGIN      
        1 cccaatctac tcaagagaca tgcgcgcgaa gaccagttac acgaccagga agtggaggag
       61 cgctggcaca agagagtgaa tgttccgcgc attgcgcgca ttttactcgc aatcatggtt
      121 ctgtctgtga tcgcatctag ctttggtttc acgcgcgtga agtgggacaa tgactccaga
      181 aacatgatcg actacaacat cgcctccgaa agactgcagg aagaaatcgc ccttcgctat
      241 gaactgacag gttctcctgc cgtgattgcg accaaatccc tggatgaggc ccgcgcgctg
      301 tttgaacact ttgaaccgcc gactcctgca gtggcggaca ttgtagcgca gacgatttct
      361 atctgggctt acgttccacc ttaccagcag caggtgcaga atagtgagct gatgcagaag
      421 tttcgtgcgg actcaagcca gattaagcca ggcatcttgc ctccgcagta caggcatttc
      481 tatccaatct atcagacgat catagggcag aagccgtttg gagtcaaaga ccttccgaaa
      541 gataccatca gccagttcga aagcaagccg gagtccaaag agaagggctg gttgactttc
      601 ttttatccgg ttgcaagcaa actctacacc gtagatggcc tcaatcaaat ggagagacag
      661 accgggaaaa tcagtgttcc tattgtgggg cgtcagtctc tcaagagcct cgcgtacttt
      721 gctccaaaac tcgaagtggc gcttcgcaag aaagcaggcg gagacgcagc gaagaaggtg
      781 agcctggatg gaatggaatt cactgaaaga gaagtatcgc tcgccctgtt tgcagccaat
      841 tctggagatc agaagttttt gaaatcaacg ggactctctg atcttgcgat tgaaaccatc
      901 ctcaagcaca gaccgtttgc gaatgtagag gcattgcaaa atgaaaagct gtctgtgcag
      961 ggaaccggat ccgttttgat tgtagctcga tttgcgaatc tggttctgga agaatccaaa
     1021 accatcatct tgatgagtct ttctcttgtg gtggctttgc tctatcttag ctttcgcgac
     1081 ttgcgctcta ctctgattgc tctggttcct atgattgcag gccttgtgct gatgatcggt
     1141 ctgatggcca tattcaatgt caaagtgaac tacttcaatg tagcggtttt acccattgta
     1201 atcggatacg gaatcaacaa cgggtttttc ctcttctatc gttttatgga atctggctcc
     1261 atcttccaga ccatgttcca tacgggtgtg gccggtctgg gctcggcgct gacttctctg
     1321 gcgggctggg gggccatcgc atcggccact catccagggc ttcgctctat gggctatctt
     1381 gctttccttg gcctcagtgc catgacgatt gccatgctag tgtttcttcc tgcacttctt
     1441 gctcttctca actcccgtta tccggcattg atagagagac tgaggctcag acacggtggg
     1501 actgcaaagg cagacccgtc tgaaatcgaa gtgccggtgg ctgtgacttc agctgcggaa
     1561 ggatctacaa aacgcgccaa aaaaggaaaa tgattccgat cagcgcgtga ggtacaggct
     1621 tactttcttt attacaccct gcggaatctc atggccgccg gagaaagaaa tgaattctcc
     1681 ggataggccg ccttcgcgaa ggaccgtttc gagtcttttc gcattttcgt agggtagcac
     1741 tggatccatc aatccatgag attgtatgaa ctggcttcct cttcgcgctg gtgcgagtcg
     1801 tgtccactga tcgcgacaga ttagatttcc agacatgatg accagtcctg ccatggtttc
     1861 agaagcgtga aggtatgtgt ctacggcaag catcgacccc tggctaaagc cgcctaacgt
     1921 gatcagattc gcaggtctcc cggtctcgtg gatcagagct tctagttttt ttctggatgc
     1981 ttcgaggcct ggaggaacta cttcataaaa attcagatgc tttccagtcg cggcaagccg
     2041 tgcgatttct tctgcgacca gtggaaacca ggctctgccc attcttcctg ggccgattgg
     2101 aaattcaatg ggtgcgtccg ggaagatcca tgtagtgccg gccggcgcgc tgagcgcttc
     2161 tgccagtggc aatagatcct gcgcatttgc cccaaaacca tgacatagaa cgacaaccgg
     2221 acctttgtct tttccctcta ctacgaaact ctccagctca gcgattctct ttttttgaac
     2281 tttcaacgtt ttctccggta caatacgtgt ggtgcaagat gatgctcgcg cgcgagttga
     2341 ggatgaagaa agtcttcctc tggatggtaa gccattccaa ttttttccat gacgcgacga
     2401 gaaggcaagt tgcctttgaa tgtaaaagat acgagttcac tcactcccag atgtgcgaat
     2461 gcatagttca cggttgcaag agctgcttcc gtggccaggc ctttgccctg aaaatcaaac
     2521 gcaagtctcc agccaatttc cacacacgga gtgaagtgcg catcaaaagc gggaacggca
     2581 gctccggtga agccaaaaaa cttaccggtg gaaatatctt cgagcgcgaa gagagtaaac
     2641 ccgtgtttct tttcatgatc gtcgattcgc ctcatgagtt cgatggtttc ctgtctggtc
     2701 agagtggccg gaaaatactt catcaccctt tcatctgcgt tcatggccgc aaattcatcc
     2761 aggtcggact cccgaaatgc acgcaatttg atcctggaag taaaaatctc cggcatcatg
     2821 ctcatgcatc aaagcagagc tttttcgatt cttgccagat gatgctgcct gtgcagagat
     2881 ctcctgaacc accggtcgcg cccatttgct tccaggaagt tgagttgatc cgggctcaga
     2941 gaaagagcaa tcgaatcaat ttcgtctgca gcttcctgca ctgttcgaat tccttccact
     3001 ctgccgacgc cgcggctcaa gaatcgaact gaattgttga tgcattcaat gttgtcggcg
     3061 tctggaactg cagtgagagt tccatcgccc atccatttct taattcgcac agaagtcatg
     3121 cgatcccagt acgctagatg gcacagaagt gtacctacag tccagtcacc acccagatcc
     3181 aatgaccagt gatggtctgt gagtcgggcc atgatggccc tcaggcgatc gttttcactc
     3241 ttgttgtcct gggcgaagct catgtgcgga aggaagcatt tgatgagctt cagtcaagtt
     3301 taattgttcc tttcgggcat gccttcggcc aggtgactca cattcactct gccagttttt
     3361 ccgcatcgcc aaatcgcacg atgtctgccc tggaagtttt tagccagtct gctgcgtgga
     3421 tcgcaggcga agatttctcc tggtcagaat ccagaatctt gattccagcc ctggtgatct
     3481 ttgcaagagc gtctgcctct tctcccagtt tgattttgaa atagtaatag gttgcgagtc
     3541 ctgctgcgag ttgcagacct tccgcgcctt cccaggtttt tcccgattgt tccccgagtg
     3601 ctttttcgct ttgagtgaga aatccattca gtacgctccg cacgcccgta gaaaaagtaa
     3661 catctttgag attcacttta ccctgggcct gatccatcac tgcgattcta gggatcatgc
     3721 tcaatccgtg gccgctcacg tagactccag tcagcgaaaa aggacccact ctctttccgt
     3781 aaaatgcttc gcagggaatc aggattcttg agggtccgct cccgctttgc acttcaggcg
     3841 cattgtatcc gcttcgcgcg atttcaaatg gaagtggctg cactgcgtca ccggatgctt
     3901 tgaagaaagt aagagtgatt gatcctccgc tttctccgcg atcaaacgtt tgcgcgtaga
     3961 gtagttctcc tgatgttccg cgcatggggg ctacgttcat gattctctgg tgtccataat
     4021 cccacgcctg caaacggagc agggggcccg ggtccttctt tttctggaat actagaaggt
     4081 gtctgtcatc gcttccagtg gaaaagattc ggaaggtttg ccaatctaac tggatcggag
     4141 atgtttcctc cgcatgcagt cccagacaga cggcgaacgt aagtacaagc agtgttgtgc
     4201 gaatcataaa atctctagat aggacgacgg caggggctct ttccactttt ttgtttttga
     4261 aatttttaac aaaatagaag caagttcgcc gttgggcaga tggctgatga cgtcgtggat
     4321 gatggtctcg cgggccagat ccggggcgat ggctccgttg agaaggggaa agcgaaagcg
     4381 catcaaagaa tcagggtcga ttcccgcatc ttcatcctcc cagctatttg cagatatgat
     4441 gccttttccg ttttcccaga ctgctatgcc gccgtcgttt gaatgaaaac gcagcgatct
     4501 catgatcagc tttgagtcat gaatcagagt gagcgcacgc gaaatgaagt ccttcttcaa
     4561 ataggacgcg aagacaactt caaagatgtc atcctgtgcc gttctgctga tgaagctgag
     4621 cgtctctttg ctcataaatc attctcccac cattccagga gattgtcaaa gcggaatttt
     4681 gctttctgtc tcagtcaatg acacggatgc ggatctggcc gccgtcttca atatctcgga
     4741 tctgacggaa gaaatccagt tcaaagtcaa tctgggcatt cgcttccact gccttgagag
     4801 acactccctg tgccgattcc tttgtgcaat agggtggaaa cgcgtggatg agctgccctg
     4861 gctcgagttt ctttccctct ttttcaaggc gaagcagagg gtgcatttgc aagaactcca
     4921 ccgggtcctc gtttgcactg gcaaagaaac cgtccaggga aagaccaagc ggatcgagaa
     4981 acgcggtctc gagcgtgagc ctgtacacca ttccgtctct gagtatgaac tgatctccca
     5041 tgcagtcttc cgcgaacgga atgtcttttt cgcccagtag ttcatacgtt cgcctcaggc
     5101 tgaattttcc atcccagatg gtttccaggg aatgccagga aggagcgacg catgcacctc
     5161 gcacatgaag acctccgcga aacgcagtga agccgttgat cttatggaga acggtgcgaa
     5221 gatcctcagg gagtctggct tccatggacg gatccactgg ctctgtacac ttgaattgaa
     5281 tgttttgtag atcgatcatg cgcttccggt tggaaattga aatctgcccc gcgaatctgg
     5341 atggagagtg tgaactgctg tcaccgcatc tagattgatt gggccgtaga cgtgggggaa
     5401 aagagcgtta gacgattttg catccgaagg cgcctcgaac cgtatatcag ccgttaggcg
     5461 atcctcttct atttccagta gaagaaggtc atgcgcttcg gaaaagaaca ggtttgcagt
     5521 ctccgccact tgatccgccg tagaacaatg cacaaatccc tctgtctcca gacttgaagg
     5581 gcgaacagag ccggcgagcc ccgcggccat ccagtcatcg cgcgaaatga tatggaaaat
     5641 cgttctgggc gactttttgg gaaactggcc cgccattgcc tgattgaact gtgcgcttgc
     5701 tcccggtgga atcgtaagcc atttccattc tccctctttt gccagtcgcg ccaggtatag
     5761 aatttgacca acatgatatg aggtgtgtgc gagagatcgc tggatggcca gaggcactga
     5821 gtgcggctct ccgcggatgg tcacggtgcg cgatacgtcc gcaggcccaa gggaggaaag
     5881 agtatccagt agcgcagccc atcctttttc ccagaatgcg gtcaggaccg ctctggtttc
     5941 gccggattga attacgaatt cgccgtctcg atttcgattt tctttttctc cgtccgtggt
     6001 caagaagtcc gtccaccgcg atagaaggtt tccggaaaca tgctttgcaa tcgaagcgat
     6061 ggacggagac cggggtccga aatctttgaa atagaattca tctttgtcca gttgcgcgat
     6121 cgctttgtct gcaagttcct tgtactgacg aaatacgcgg atggtttctt gtagatagat
     6181 gctatttgag tccatggatc atcctttgcg cgtttgtgcc gttagccgct tttagtcgaa
     6241 cccggtctgc cctaccgccg gagtagtgag tttctttctg aaatagatgg ctgattcttc
     6301 gtaaccaatg gagcgataaa aatcggaggc gcgtctcgtt gccagagcaa ctagctttcc
     6361 tcctctggat gtaacccatt gttcgaaatc tactgtaagt gcccgtgcga ttccctggcg
     6421 tcttaacctt tcattcacca taatttcttc tatccatcca acgcttccgt ttgcaaagaa
     6481 agtaagatga gtgaatccaa gcaagtaacc cacaatcgtc ttttgtgaca cggccacgag
     6541 tacgcgagcg gattcttcgc ggatcaattt ctcaaacgag tttgaaaagg tgactctgtc
     6601 tggttgaaac gatactgcga aatttcgcac aagcccgtag acttcatctt cgtccaggga
     6661 gcgggctttg cgaatttcaa actccatgct caaatcattt ggttagaaca atgtgcgtcg
     6721 cattctttgt agttacgtgc tctgtgattt tataacccag agccaccagg tccacgccct
     6781 cgaaaagtct ttctcctgat cccagaatgg tcggggagat ggcaacgtgg agctcatcga
     6841 tcagaccttt gtttaagtac tggcgcacaa gagaaactcc tccgcccaat cgcacatcct
     6901 ttccattggc tgcggcgaat gcctgcttga gcgcggattc gattccatct gttacaaaat
     6961 gaaaagtgtt cccgccttcc atcggaatgg actggcgcgc atggtgagtg agaacaaaga
     7021 ccggactgtg atacggtgga tttggacccc accagccttt ccattcgtcg ttgggccagg
     7081 ggccgcgaat gggaccgaac atgtttcggc cgagtatgtt tgcgccaatg ttttcaaatc
     7141 cgcgcgcggc gaaatcgtaa tctactcctg tttccccttt gctttcgccg aacatttgtt
     7201 gaaatgtcct ggtaggaaaa aaccagctgt gtacatctct gcctccaatc ccaagtggat
     7261 tttccaggct ctgctctggc ccggcgccaa agccgtcgac tgaaatggag aaacaacgaa
     7321 ctacaagttt ttgcatgatt atatcctgag agatccgcga aagcctcgca ccgcgtaata
     7381 agactctgct ccgttatgat aaaggaaaac tctaccgtag cgacgatcgc aaaagacggc
     7441 ccctcccagt ttcctcacat ccgccggcgt tttcacccag ctggacgttt tcgtgtcaaa
     7501 ttcgccgagc tcctggagtt gtctgtattc ctcttctgtg agtagctcga tgcccatcgc
     7561 atttgccatg ttcacggcgc tgtctttggg tttgtgttcc ttcctggatg cgagcgcttc
     7621 tttgtcgtag caaa
//
LOCUS       DAOQJL010000133         7554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5331_length_7554_cov_4.336178, whole genome shotgun sequence.
ACCESSION   DAOQJL010000133 DAOQJL010000000
VERSION     DAOQJL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5331_length_7554_cov_4.336178"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..720
                     /locus_tag="PLG78_07720"
     CDS             127..720
                     /locus_tag="PLG78_07720"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNE23039.1"
                     /translation="MIRDKEDRCPWCLKDPVMLKYHDTEWGVPQTKDKKLFEFLILEG
                     AQAGLSWITILKRRAGYAEAFAQFDPEKVATFPKRKIESLLGFEGIIRNRLKVESAIT
                     NAKAFLKVQEEFGSFNQYQWQFVDGKPKQNSWKAIGQVPATTKESDAFSKDLKSRGFK
                     FVGSTIMYAHMQAVGMVNDHLVGCPRHAAVSRISFKI"
     gene            790..2907
                     /locus_tag="PLG78_07725"
     CDS             790..2907
                     /locus_tag="PLG78_07725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23040.1"
                     /translation="MLFRAAILLFLAAGTLVAAPLPGKDPVLLGPYMGIAHDRGLTIK
                     EVTAPDFASEFKAAPSPIPNFGMTDDAYWTRFDLANPSPSPRSLMVEVAMPHLDDVRL
                     YVPEGRLFHEYVSGRNYPFSTRAVAHENYVFPVELPPYFQGTLYMRFEQEGTFQLPVY
                     LWDADNFHQADRLKQLVLGSYYGVAIVMLIYNLFLWIAIRERAYIYYVLAILFTHFLV
                     VFHINGFSYVWFFPESPVIAKRSYPVLLNLAGYFSIQFVQALLLTKERSPRLDRGLVW
                     LRRFALITSFFAVFASYFLAYKVTVYLALVMAVALIASATYRAIQGDRIAVYYVVAWS
                     VLVAATVLLILRTVGVAPVNFATDYGVQVAASLEVVLLSLALGERINEMRREKDRILI
                     DLREKQQSQQLAVALRQLTAKISSTLNHNELIEHVKVSLPDIVRADQVRLLLKSAKDE
                     LTIPDAESNGGSLDLAEMTRAMDKSEPSILQTDRAHLVAVPVHSRSVPRGLLLLRRSE
                     RFPQDEVDILRTVASEIGMVLENIRLFSEMHEMATVDSVTGLLSRAHFMNLASVEFER
                     SRRYKRPLSALMIDIDYFKQVNDLYGHQAGDEILTRIATLCKSSLREVDIIGRYGGDE
                     FSVMLPETPPRGARDVAERIRKRIEKESLKFTGKGDLQVTLSLGTATVDKKTDSLKTL
                     LSRADAALLEGKKAGKNRVAAAR"
     gene            complement(2909..3673)
                     /locus_tag="PLG78_07730"
     CDS             complement(2909..3673)
                     /locus_tag="PLG78_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE23041.1"
                     /translation="MIDVVRNGHILELYIQTNDTNSMGLEFFRALGANIEEAEVDRGI
                     KAVMLSSRNEKFFSNGFDPSIFIGRSREDVYSVLQSALDASGKVLFSKKPVVCAMNGH
                     AMGVGAVLAIFSDYRILVEKKGRIGFPESLIGINFPSTSAYVLTQLVGIRTARDLLYS
                     GRALKSDEALSLGLVDESASPEEVLPRARAWCAQFENMAPESVTGIKVALRDSQRVIA
                     AELASNDLEFLAEAVMSPNGQEGMRSILEKRRPKFS"
     gene            complement(3686..5581)
                     /locus_tag="PLG78_07735"
     CDS             complement(3686..5581)
                     /locus_tag="PLG78_07735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23042.1"
                     /translation="MGTVSSDDFSDRRRPKMESKQSDQRGAEEVHIPLEVVQQAGSGI
                     SGLLAASEVETTLLLLNAIADGVIGLNRSGMIIYANQSARGLLGYSSAELMGRRLDEF
                     LIPPQSIEKILDPVLQGDVVSTDETILLSRKASTMDVIITGVPLIQHGQVSGALVIFR
                     DITPGILLNRSLKEREEILRGIFYILPSGIMLMGQDGRIVRINEAARGLLSLGQAELR
                     GEPYPATLWQAQDLDGNEIPIEEAPFQTALQSGLPVVGKVLRLGYHPNERFVLMSSSP
                     LNVPGAPESRMVVTTVTDVNQQLKVQSRLETAVSLNNLLKNLLTETVPEETVDTALRG
                     VCELMNADLALLGLLDDERKQITYDHILGFPEVFRGYTRPFSGSICEEMFARKMPVTI
                     AKYSADPRRIDALVRAGAMTMLAAPILADQELIGSILMFRKVERPFESTDGESLMTLT
                     PVLSAALYKATYERKLRELATSDPLTGIWNRRVVYEHLDMEIERCRRYGASLSILVLD
                     LDHFKLVNDTYGHQAGDAVLITFSQLLRRLTRRTDMVGRSGGEEFMVILPDTSLQGAL
                     KTAEKIRVDFEHSPTQYRDLTIPATVSIGCTEYIRGESFEDFFGRVDRLLYRAKAAGR
                     NQVVVDD"
     gene            5653..6519
                     /gene="mtnP"
                     /locus_tag="PLG78_07740"
     CDS             5653..6519
                     /gene="mtnP"
                     /locus_tag="PLG78_07740"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNE23043.1"
                     /translation="MAESAKIGIIGGSGLYGLEGLRVETEVTPETPWGKPSDAIVIGE
                     YAGKRVAFLSRHGRGHVYSPSEVPFRANIAALKMLGVEQIIAFSAVGSLREEIAPRDF
                     VLPVQIIDRTKGIRASTYFENGVVVHATFGDPFDGQLATLVKQAAARVNGLKLHADKV
                     VVCMEGPAFSTRAESQLYRSIGGDIINMSVLPEAKLAREAEIPYQMICMSTDYDAWKE
                     HEEAVTTELVIGHLNANAESARKLLAELIPLLGEGANPLKGSIKNAVITAPEKRNPEQ
                     LKKLAKLLPGTF"
     gene            6520..7221
                     /locus_tag="PLG78_07745"
     CDS             6520..7221
                     /locus_tag="PLG78_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23044.1"
                     /translation="MIRTLVAVSVLVMALPLSAQLSTDTRPTTGSTPDTPGTSNNSIL
                     PGAGTVPALQERRPTPRDPRYRPPVSVPGLKEPSNPAALYLFSGDVDAQKFMDSRNKQ
                     KKASRYDEADYDDLPAGGRERVWREKEMLRQFGVDHFPYAGPEQEEPESDLRRFQIVF
                     FISLPITAAASYGLFRAAKGQSAGGFSRGETMGVFALGLGLSGAIGAYDYYQNHLTQR
                     SANIELFHAAVTFRF"
     gene            7269..>7554
                     /locus_tag="PLG78_07750"
     CDS             7269..>7554
                     /locus_tag="PLG78_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23045.1"
                     /translation="MDEPIIYAGSTAVDASVPEELPLVFLIEGDPALRSDVSTQIHYS
                     GYRVLAFEKIESALEECRRKVPAVMVVDLSQPEGDMAMVLAEIQMLNEHSI"
BASE COUNT         1955 a         1937 c         1863 g         1799 t
ORIGIN      
        1 ggttcgcgca gaaaccattt tgtgcgacga aaacacagta aaagcggcag gaagtcacgt
       61 tcaattcgaa caacaaccgg acctttccct gaatcgactc ggcggagtcc gaacatttcg
      121 cccactatga tccgcgataa agaagatcga tgcccgtggt gcctgaaaga tcccgtcatg
      181 ttgaaatacc atgacaccga atggggtgtg ccgcagacaa aagacaagaa gctatttgag
      241 tttctcatcc tcgaaggcgc tcaggcaggc ctgagctgga ttacgattct aaaaaggcga
      301 gcgggctacg cggaagcatt cgctcaattt gatccagaga aagtggcgac attcccaaaa
      361 agaaaaatag aatccctttt gggttttgaa ggaatcatac gcaatcgtct gaaagtagag
      421 tctgcgatca cgaatgcgaa agcatttctg aaagttcagg aagaattcgg gagttttaat
      481 caataccagt ggcaatttgt agacggaaag ccaaaacaaa atagctggaa ggccatagga
      541 caggtgcctg cgacaacgaa ggaatcagac gcattcagca aggatctaaa gtcgaggggc
      601 ttcaagttcg ttggatccac catcatgtat gctcatatgc aggcggtagg gatggtcaac
      661 gatcatctgg tcggttgccc cagacatgca gccgttagcc gaatcagttt taaaatctaa
      721 actaattcaa tttcgtcctg aattcgaaaa agaatcgaca caacaacctc ccgtcttcag
      781 gctcatcgcg tgctttttcg cgcagccatc ctcttgttcc tggcagccgg cacactggtc
      841 gcagcccctc tacccggaaa agatccggta ctgcttgggc cgtatatggg aatcgcgcat
      901 gaccgcggac tgacaatcaa agaagtcact gcacccgatt tcgcgagcga atttaaggct
      961 gctccttctc ccatcccgaa ctttggaatg acagatgatg catactggac cagattcgat
     1021 ctggcaaacc cctccccttc tcccagaagc ttgatggtag aagtggccat gcctcatcta
     1081 gacgatgttc gtctctacgt tccagaaggg agactctttc acgaatatgt atctggaaga
     1141 aattatccgt tcagcactcg cgcagttgca cacgagaact atgtattccc ggttgagctt
     1201 ccgccctatt ttcaaggcac gctgtatatg cgattcgagc aggagggcac gttccagctt
     1261 ccggtctacc tctgggacgc agataacttt catcaggcag acaggctaaa gcaactcgtc
     1321 ctgggatctt actacggcgt tgcgatagta atgttgattt acaatctgtt cctgtggatc
     1381 gccatacgcg agcgcgcata catctactac gttcttgcca tcctgttcac tcatttcctg
     1441 gtcgtgtttc atatcaacgg ctttagctat gtctggtttt ttccggagtc gccggtgatt
     1501 gcaaaaagaa gctatcctgt gctcctgaac ctcgccggat atttctctat ccaattcgtt
     1561 caggcacttc tacttacaaa agaaagatct ccacgcctgg atcggggcct ggtgtggctt
     1621 cgcagatttg ctctcatcac ttccttcttc gcagtcttcg catcgtactt cctcgcgtac
     1681 aaggtgacgg tttatctagc actggtgatg gccgtggcac tcatcgcaag tgcaacgtat
     1741 cgggccattc agggagatcg gattgcagtc tactacgtgg tggcgtggtc tgtgcttgtg
     1801 gcagcaactg ttctattgat tcttcgaacg gtcggtgtgg ctcccgtgaa tttcgcaacg
     1861 gattacggcg tgcaagttgc agcatcttta gaagtggtgc tcctctctct ggctcttgga
     1921 gaaagaatca atgagatgag gcgagagaaa gatagaatcc tcattgacct gcgagaaaaa
     1981 caacaaagcc agcagcttgc cgtagctctc agacaattga cggcgaagat tagctccaca
     2041 ctcaatcaca atgaacttat agaacatgta aaagtgtctt tgcctgatat agtcagagca
     2101 gaccaggtgc gtctacttct caagtcggcc aaagacgaac ttacaattcc cgatgcggaa
     2161 tcaaacggtg gatctctgga cctggcagag atgaccagag ccatggacaa aagcgaaccg
     2221 tccattcttc agacagatcg tgcacacctg gtcgcagtgc cagtgcactc tcgctccgtt
     2281 ccccgcggac tgttgttgct tcgaaggtca gaacgatttc cgcaggatga agtggacatt
     2341 ttgcgcacgg ttgcatccga gatcggcatg gtccttgaga acattcgcct cttttcagaa
     2401 atgcacgaaa tggcaaccgt ggattctgtg accgggctac tatccagagc tcacttcatg
     2461 aaccttgcat ccgtggaatt tgaaagatcg cgcagataca agagaccgct ttccgcactc
     2521 atgatagaca tcgactattt caaacaggta aacgatctat acggtcatca ggctggagac
     2581 gaaatactca cacgcattgc aactctttgc aagagcagtc tgcgcgaagt ggatatcatt
     2641 ggaagatacg gtggagacga attctccgtg atgcttccag aaacgcctcc tcgcggagca
     2701 cgcgacgttg cagagagaat tcgaaagaga atagaaaaag aatcactgaa gttcacgggg
     2761 aaaggagacc ttcaggtaac gttgagtctg ggcactgcca ccgtggacaa gaaaacggat
     2821 tctctaaaaa cccttctgtc gcgcgcagac gcagcgcttc tggaaggaaa gaaggctggc
     2881 aaaaatcgcg tcgcagctgc cagatagttc agctaaactt tggtcttctt ttttccagaa
     2941 tggaacgcat accttcctga ccgttaggcg acatgaccgc ctctgccaga aattcaagat
     3001 cattggaagc cagttctgct gcgatcactc gttgagaatc tctcagtgcc accttaattc
     3061 ccgtgacgga ttcgggtgcc atgttttcaa actgagcaca ccaggcacga gccctcggga
     3121 gcacttcctc gggggatgca ctttcatcta cgagaccaag agacagagct tcatccgatt
     3181 tcagagcgcg accactgtac aaaagatcgc gcgcggtcct gattccaaca agctgggtaa
     3241 gcacgtaagc agatgtagaa ggaaagttga ttccgatgag agattctgga aatccgattc
     3301 ttcccttctt ctccactaga attctgtaat ctgaaaaaat cgcaagcaca gcgcccacgc
     3361 ccatagcatg cccattcata gcgcaaacga ctggtttttt tgaaaagagc actttgcctg
     3421 acgcatccag ggcagattgt agaacagaat acacgtcttc gcggctgcgc ccaatgaaga
     3481 tagaaggatc aaaaccattg gaaaaaaact tttcatttct acttgaaagc atcacagcct
     3541 tgatgcctct atctacttct gcttcttcta tgtttgcgcc cagggccctg aagaattcga
     3601 gacccatgga atttgtatca ttggtctgga tgtaaagctc caggatatgc ccgtttctga
     3661 ctacgtcgat catgtgactc tccgactaat cgtctacgac tacctggttt cttcccgccg
     3721 ctttcgcgcg ataaagcaat cgatccactc ttccgaagaa atcttcaaaa gactctccgc
     3781 gtatatattc agtgcatcca atggaaacag tcgctggaat ggtcagatct ctgtactgag
     3841 taggcgaatg ttcaaaatcg actcgaatct tctctgcagt cttgagtgct ccctgaagag
     3901 aagtgtccgg taagatcacc atgaactctt ctccaccgct tctgcccacc atgtctgttc
     3961 tgcgcgtgag ccttctcaga agctgagaga atgtaataag aactgcgtct cctgcctgat
     4021 ggccgtatgt gtcattcacc agcttgaaat gatccagatc cagcacaaga atgctcaagc
     4081 tcgcgccgta tctgcgacat ctttctattt ccatatccag atgctcgtag acgactctgc
     4141 gattccagat gccggttaac gggtcactgg ttgccagctc tctcaacttt ctttcgtagg
     4201 ttgctttata aagcgccgcg gacaacacag gcgtaagtgt catgaggctt tcgccgtccg
     4261 tagattcaaa gggacgctct actttgcgaa acatgagaat agaaccaatg agttcctgat
     4321 cggctagaat gggggctgcg agcatggtca tggcacccgc gcgaacgagt gcatcaattc
     4381 tccgtggatc tgcagagtat tttgcgatgg tcacaggcat ctttcgcgcg aacatttctt
     4441 cgcaaatgct tccgctgaag ggtctggtgt atccgcgaaa cacttccggg aatccgagga
     4501 tgtgatcgta agtgatttgc ttgcgctcgt catcgagaag cccgaggagt gcgagatccg
     4561 cattcatgag ttcgcacaca ccgcgaagag ctgtgtccac tgtctcttcg gggacagtct
     4621 ctgtgagtag attcttgagt agattgttga gagagacggc agtttcgagt ctgctctgta
     4681 ctttgagctg ttgatttacg tccgtgaccg tggtcacaac cattctggat tcgggcgctc
     4741 cgggaacatt gagtggactc gaagacatca atacaaaacg ctcgttggga tgatagccca
     4801 ggcgcaaaac ttttccgacc acaggtagtc cgctctgaag agctgtttga aaaggcgctt
     4861 cttcgattgg gatttcattc ccgtccaggt cctgcgcctg ccagagagtc gccgggtaag
     4921 gttctccgcg cagttctgcc tgaccgagag atagaaggcc tcttgccgct tcattgattc
     4981 gaacaatgcg accatcctgc cccatgagca taatgccgga cggtaatata tagaagatac
     5041 cgcgaagaat ttcctcacgt tctttcagag aacgattcaa taaaattcca ggcgtaatat
     5101 cgcgaaagat caccagggct ccggaaacct ggccatgctg aatcagaggc acgcctgtga
     5161 tgattacatc catagtagac gctttgcgcg aaagcaagat ggtctcatct gtggagacaa
     5221 cgtctccctg cagaactgga tctaaaattt tctcaatgga ctgaggtgga atcagaaact
     5281 cgtcgagtct acgacccatg agttctgcgg acgaataacc aagtagcccg cgcgcagact
     5341 gattggcgta gatgatcata ccagatcgat tgaggccaat gactccgtct gcgattgcat
     5401 tgagcaacag cagagtggtt tcaacttcag acgcagcaag caggcccgaa atgcccgacc
     5461 cggcttgctg gactacttcg agaggaatgt gaacttcttc agctcccctc tgatcgcttt
     5521 gttttgattc catctttggg cgacgcctgt ctgaaaagtc gtcggaactt acagttccca
     5581 tgaaaatttt tgacagcgat ggaggcaaga aaaagaaaat gacaggcttg cccgaactgg
     5641 cgagttcatc ccatggcaga atcagcgaaa attggcatca ttggcggcag cggactctac
     5701 ggactggaag gtcttcgcgt agaaacggaa gtaacgccgg aaactccctg gggaaaacca
     5761 tcggacgcaa tcgtaatcgg cgaatatgca ggcaaacgag tcgcgttcct ctctcgccat
     5821 ggccgcggac atgtctacag cccttcggaa gtgccctttc gcgcaaacat agctgctctt
     5881 aaaatgctcg gggtagagca aatcatcgcc ttctcagccg ttggatctct tcgcgaagaa
     5941 attgcacccc gcgattttgt cctacctgta cagatcattg atcgcaccaa agggattcgc
     6001 gcatccacat atttcgaaaa cggagtcgtg gtccatgcaa cctttggaga cccgttcgat
     6061 gggcaactgg ccactctggt gaagcaggca gcagcccggg tcaacggatt aaaactgcat
     6121 gcagacaaag tggtcgtgtg catggaaggt cccgcatttt caactcgtgc agaatcacaa
     6181 ctatatcgca gcattggcgg agacatcatc aacatgagtg ttttgcctga ggcaaaactc
     6241 gcgcgcgaag ctgaaattcc ttaccagatg atctgcatgt ccacagacta cgatgcgtgg
     6301 aaagaacatg aagaggcagt gaccaccgaa cttgtaatcg gccatctcaa cgcaaacgca
     6361 gagagtgcgc gaaaacttct ggcagaactc attccacttc ttggagaggg agccaatcct
     6421 ctcaagggaa gcatcaaaaa tgcagtcatc acggcgccag aaaagagaaa tccggaacaa
     6481 ctgaagaagc ttgcgaaact attgcccggg actttctgaa tgattcgtac tcttgttgcc
     6541 gtatctgtgc tggtgatggc tcttcctctc tcagcgcaat tatccacgga tacgcgcccc
     6601 acgaccggaa gcacaccaga cacacccggt acgtctaaca actcgatcct tccaggtgca
     6661 ggcaccgtgc ccgcactgca ggaacgaaga cccactcccc gcgatccaag atatagacct
     6721 cctgtatccg ttcccggact caaggaacca tccaatccgg cggctctcta tttgttctcc
     6781 ggagacgtgg atgcgcaaaa attcatggat tcgcgcaaca aacaaaagaa agcaagcaga
     6841 tacgatgaag cagactacga cgacttgcct gctggcgggc gcgaaagagt ctggagagaa
     6901 aaagaaatgc tcagacaatt cggcgtggat cattttccat atgccgggcc tgaacaggaa
     6961 gaaccagaaa gcgacctgag acgattccag attgttttct ttatttcact tcccattacg
     7021 gcggccgctt cctacggcct gtttcgcgct gccaaaggcc agagcgcggg tgggttttcg
     7081 agaggagaaa ccatgggagt gtttgctctg ggccttggac tttcaggagc gatcggggcg
     7141 tatgactact accaaaatca tctcacgcaa agaagtgcca atatagaact attccacgct
     7201 gccgttacat tccgcttcta atcttttcta ctttccaaaa ccaggcgggc ccgtatgatc
     7261 aggccgcgat ggatgagcca ataatctacg cgggctccac tgcagtggac gcaagcgtac
     7321 ctgaagaatt acctctggtt tttctcatcg aaggggatcc ggcacttcgc tcggacgttt
     7381 ccactcagat tcattacagc ggttacagag tgcttgcttt tgaaaagata gagtccgcgc
     7441 tcgaagaatg caggcgcaaa gttccggctg tcatggttgt agatttgtct cagccagaag
     7501 gcgatatggc catggtgctt gcggaaattc agatgctcaa tgagcattct attc
//
LOCUS       DAOQJL010000134         7552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5337_length_7552_cov_4.288649, whole genome shotgun sequence.
ACCESSION   DAOQJL010000134 DAOQJL010000000
VERSION     DAOQJL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5337_length_7552_cov_4.288649"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLG78_07755"
     CDS             complement(<1..586)
                     /locus_tag="PLG78_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23046.1"
                     /translation="MKPFVLFLAALTLFTAECKQGGKISQSTETRVTRFRMGVLKTMG
                     DPKSALPLSLEKGEVVTLDRIESYKPAQGAAVEYAKIKIAGGQEGYVVASYLAKSGVV
                     ITGDAKLYQRPNITSGLARGNSNLTVGTVAFVENEEFSDGEWVEVSGGANSASYFRGW
                     LKGGAGISKDTELVTAAVRFEQAAEIYKNPKASSK"
     gene            complement(666..1496)
                     /locus_tag="PLG78_07760"
     CDS             complement(666..1496)
                     /locus_tag="PLG78_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23047.1"
                     /translation="MFKPLYLLIAFISLANCESILSRRPTIDFVDADVTDLTMKYIDL
                     RLKLKLNNPYPIKLPPGALGGDLSLDGKTITGITTTTPEVAASGAGTFPLQIRIPFES
                     IMNLGQDLEKKEEFHFRVKGNADVQVTALAGAPAARLNLPFDYEQNVPAFVPDITFGN
                     LQLETPSMASPSAGVSFDLGVRNKAAGKFNLGKMDFQLLSNGMSLLNGSTQEVVNSGT
                     ETKARIVSKVSLLAVPFALLQGSKNFTVKGGTTLSFPGFFGGKTFPLQFEKPLQESAR
                     "
     gene            complement(1489..2586)
                     /gene="fliG"
                     /locus_tag="PLG78_07765"
     CDS             complement(1489..2586)
                     /gene="fliG"
                     /locus_tag="PLG78_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNE23048.1"
                     /translation="MQKRSIDVLISDEGILPTNPVPQMKRPAQTPVPPTSDSGATRAA
                     KLLLSLGPDQASVILREMEPAEVEKVVSEMIRIRSITATEKKSILGEFHAIVEDFDPP
                     VRGGIDSATDLLVRSLGEQKAGEILSRVNRRDVRNDFAFLEGIDPNLLSTVLSAEHPQ
                     VVAIALASIHPRTAASVLRSFPDELRAAVSLRIAKTSKTHPDALEKVARVLREKFEKR
                     KDEIYSEVGGADTLATILNHMDRDLEDNILKELETAAPDLLTTVKEKLYTFEELIALT
                     PKEMRLLASQINDDSILACALRGAGEDMRRMFFNNLSQNRAADILEEIDRRGPISLRE
                     IHEARGFILSAARRLDDEGRIVIKKSKEEYV"
     gene            2697..5228
                     /locus_tag="PLG78_07770"
     CDS             2697..5228
                     /locus_tag="PLG78_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNE23049.1"
                     /translation="MNGIPGLPEILAPAGGREQFFAALGAGADAVYLGLKGFNARARA
                     ENFTLEDLRELVPLARTSGMRVLVATNVVLKQDELPLIMDAIAELDWIGVHALIIQDL
                     GLARTIRERMPWMRMHASTQLAVHNEDGAAFAADMGFKRVVLARELTARDISRIKEAV
                     GDRIELEVFCHGSLCYSYSGLCFFSGAEDARSGNRGECAYTCRKPYRIVSEPGYGFLF
                     SMKDLATSDHVDALLGAGVSALKIEGRKKDAQYVASSVGLYKQARDRALGEASGAARN
                     FSEDLKSSYQRDTTSFFVKGRYHENVIDLDNAGHRGTRIGVVQSAGKGWMEVTTSEPL
                     DLFDGLRVDSPDQVYHALPQQGQNLRGSVKKAQAKYENEICQFSLREMQKGSKRMTTA
                     LAGDHLRIQIPSDARVKEGDVVFRVRSNELKRYVDSISSESGRLRSLYLMRMLVSIQD
                     GPPLQLRASLFDHSGEIVSASIQTEASRPRGTSTLAQDLRETFALLGDAGVECDVEVE
                     GDPEWFVPRSKIKELKRMLSSQALQSMPAILAARKRDARPSSQGSAPVARATTYQIKV
                     DRLEYLSALDQILTSHPGLVDEIIFEPKKSNISKSSPTDVFASLRSVADKHSVHVRFA
                     IPVVIRSWDEGHLRAYVNEYVLQGGSRFEVANVGAFKFLQDHKIALTDLASDFTLYAL
                     NSQSVLSLHELGCNRVALSVEDDWQNLQRLLAQWPHQEILPQAILYKDTPLFIAEACS
                     LTALHNGCPTSKVCGYRTLEIENDEGDRFFVAHEQCKSIVFGKNAYSISGRRSLLEGA
                     GVHRFRLDFLTRLYSKEDILRIANLAFKDQQIANTHPANFHRKLL"
     gene            5265..5558
                     /locus_tag="PLG78_07775"
     CDS             5265..5558
                     /locus_tag="PLG78_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23050.1"
                     /translation="MNLLMRIFILLSLLWMIGCKDLRDGYEKGFKEEFQKSFTKSCAS
                     SAVKKGMNEAKATEYCACTAKELASRHSSQELMKMAASDKDGEVAAAVKACTK"
     gene            complement(5539..6198)
                     /locus_tag="PLG78_07780"
     CDS             complement(5539..6198)
                     /locus_tag="PLG78_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23051.1"
                     /translation="MDASEFPYRSVRRTLLAWFKKNARDYPWRKTGNWFHLTMAEMML
                     RRTRADQAARVYAKFTSRFTTAQSAQANREEVLAILAPLGLKWRTKQILETIDYLKDT
                     YKTRKPSANDDLTRIPGVGEYSNAMIRNRLYGERLPALDSNMARLICRISGKKFTQES
                     RRNRFVSDVAHSLVQTKHSRDLNLAVLDYCALVCKPTRPLCGQCKLISKCKYGATSYR
                     P"
     gene            complement(6173..7264)
                     /gene="dcm"
                     /locus_tag="PLG78_07785"
     CDS             complement(6173..7264)
                     /gene="dcm"
                     /locus_tag="PLG78_07785"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNE23052.1"
                     /translation="MQKPKVPGSRSVSTDHTSMRPQSRGIARANNRRNYKIIDLFAGI
                     GGIRLAFENAGYSSVFSSEWDKSAAFTYAANFKDTPAGDIRAVDSRTLPDFDVLCGGF
                     PCQPFSIAGVSKKNALGRPHGFADETQGTLFFEVLRILKDKKPSALLLENVKNLTSHD
                     KGRTMQVILAALKEAGYHVHHSLLNARKLLPQNRERIFIAGFRNLQSFQAFQFPELKD
                     RSPRLSDILEKNVPAKYTLSDHLWQYLQDYKAKHEAQGHGFGFGLASRDGISRTLSAR
                     YYKDGSEILIPSRGKNPRRLTPRECARLMGFPDHFKIVVSDTQAYKQFGNSVAVPVVQ
                     AIAENMKKALDAATKTQRTSAHGRIRISV"
     gene            complement(7304..>7552)
                     /locus_tag="PLG78_07790"
     CDS             complement(7304..>7552)
                     /locus_tag="PLG78_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23053.1"
                     /translation="HKPSSALKMGVSRTGDSCRLTYNRQGKTFSKEFRTPHGSYPDLG
                     CVAQCYTNENLIELESLSPLTDVPPGSALEHLETWSIS"
BASE COUNT         1742 a         1820 c         2009 g         1981 t
ORIGIN      
        1 ttttgctcga tgctttagga tttttataaa tttctgcagc ctgttcaaat cgcaccgctg
       61 cggttacaag ttcagtatct ttggaaattc ccgctccacc tttcagccag ccgcggaaat
      121 agctcgcaga gttagctccg cccgaaacct ccacccattc tccatcactg aattcttcgt
      181 tctctacgaa cgccacagtg ccaacagtaa gatttgaatt tcctctcgca aggccggatg
      241 tgatgtttgg acgctgatag agtttcgcat ctccagtaat gacgactccg ctttttgcaa
      301 gatagcttgc gaccacgtag ccttcctgac cgccggcgat tttgatcttc gcgtattcta
      361 ctgcggcgcc ctgagctggc ttgtacgatt caattctgtc gagagtcacc acttcgcctt
      421 tctccagaga aagaggcagc gcgctttttg gatctcccat tgttttgaga acgcccatgc
      481 gaaacctggt cactcttgtt tctgtggatt gcgaaatctt gccaccctgc ttgcattctg
      541 cggtgaagag tgtgagcgcg gcgaggaaaa ggacgaaggg tttcattgca ttctctctga
      601 aggtgattgg tcagagatgc gtccctgtgt cgcgggacgc aagcatttag cgagaaccgc
      661 cagacttaac gggcgctttc ttgcagtggc ttttcaaact gcaacggaaa tgttttccct
      721 ccgaagaaac cgggaaaact cagagtggta ccacccttca cggtgaaatt tttacttccc
      781 tgcaacaggg cgaaaggcac tgcaagaagg gaaacttttg aaacaatcct ggcttttgtt
      841 tctgttccgg agttcactac ttcttgagtc gatccgttga gcagtgacat gccattggag
      901 agcaattgaa agtccatttt tcccagattg aatttcccgg cggccttgtt tcgaactccg
      961 agatcaaaac tgacgcccgc ggaaggagag gccatagacg gcgtttccag ttgcaaattt
     1021 ccaaacgtga tatctggcac aaatgcgggt acgttttgct cgtagtcaaa cggaaggttc
     1081 aatctcgcag caggagcgcc ggcgagtgcc gtgacctgga catctgcgtt tccttttacg
     1141 cgaaagtgaa actcttcctt tttctccagg tcctggccca gattcatgat cgattcaaaa
     1201 ggaattctga tttgcagcgg aaatgtgccg gcgcccgagg ccgctacttc cggagtggtg
     1261 gtagtgatgc ctgtgattgt ttttccatcg agagaaagat ctcctcccag agcgcccgga
     1321 ggtagtttga ttggatatgg attgtttaac ttcagtttca atctcagatc aatgtatttc
     1381 atcgtgagat cggttacatc cgcatccacg aaatcaatgg ttggcctacg actgaggatg
     1441 ctttcgcagt tggcaagcga gatgaaggca atcaggaggt agagaggttt aaacatattc
     1501 ttcctttgac ttcttgataa caatgcgtcc ttcgtcgtca agtcgtcttg ctgcagaaag
     1561 aatgaatcct cgggcctcgt ggatttctct gagtgaaatc ggaccccgcc ggtcaatttc
     1621 ttccagtatg tctgcggccc ggttctgcga gagattgttg aagaacattc gcctcatgtc
     1681 ttctccagcg cctctcaaag cgcatgccag gatagaatcg tcattgatct gcgatgcgag
     1741 aagtctcatt tcttttggag tgagagcaat gagttcttca aacgtgtaga gtttctcttt
     1801 cacagtggtg agcaggtcgg gtgcggctgt ctcgagttct tttagaatat tgtcttccag
     1861 atcgcgatcc atgtgattga gaatcgtcgc cagcgtatct gcgccaccca cctctgaata
     1921 gatttcgtct tttctctttt caaatttctc acgaagcacg cgcgccactt tctccagtgc
     1981 gtccgggtga gtcttgctcg tcttggcgat gcgcaatgat actgcagcac gcagttcatc
     2041 cggaaaagaa cgtagaacgg atgctgctgt tctgggatga atgctcgcca gtgcaatcgc
     2101 tacgacctgc gggtgttcag cagaaagaac tgtggaaagc aggtttggat ctattccttc
     2161 gagaaaagcg aaatcgtttc taacatctcg acgattgacg cgtgaaagaa tctctcctgc
     2221 cttttgttcg cccaggctgc gcacgagtag atccgtggcg ctgtctattc ctccgcgaac
     2281 aggtggatca aagtcttcta caattgcatg gaattcaccc aggatacttt tcttttccgt
     2341 cgccgtgatg cttcggattc ggatcatttc cgagaccact ttttccactt ctgcaggttc
     2401 catctcgcga aggatgacag acgcctggtc cggtccgaga gacaacagta gcttggccgc
     2461 gcgcgtggcc ccggagtctg aagtaggagg caccggcgtt tgcgccgggc gcttcatctg
     2521 gggcacaggg ttcgtcggga ggatgccctc atctgaaatc agaacgtcga tgcttctctt
     2581 ctgcatgact cactcatgtg acataatcca gaggaggcaa gttgattttg cagcgaaact
     2641 gcgattgtct attttttctg atcgccccgg ggcgtgattc tgctgtcttg attggtatga
     2701 acgggattcc tggattgcca gagattctgg cacctgcggg gggcagagaa cagttctttg
     2761 ccgcgcttgg cgcaggtgca gacgcagtgt atctgggact caaaggattc aacgccagag
     2821 cacgcgctga aaatttcact ctggaagatc tgcgcgaact cgtgcctctc gcgcgcactt
     2881 caggaatgag agtgctcgtg gccacgaacg tggttctcaa gcaggatgaa cttccgctga
     2941 tcatggacgc catagcggag ctggattgga ttggagtgca tgctttgatc attcaggacc
     3001 tcgggctggc gcgtaccatt cgcgaaagaa tgccatggat gagaatgcac gcgtccacgc
     3061 agcttgcagt acacaacgaa gacggcgctg cctttgccgc agatatggga ttcaaacgag
     3121 tcgttcttgc tcgtgaactc actgcgcgag atatctcgcg aatcaaagaa gcagtgggcg
     3181 atcggattga actggaagtg ttctgtcacg ggagtctgtg ttacagttac agtggtcttt
     3241 gttttttcag cggtgcagag gatgcgcgaa gcggcaatcg aggcgagtgc gcatacacgt
     3301 gcagaaagcc atatcgaatt gtgagcgaac caggatatgg atttttgttc agcatgaaag
     3361 atctggccac gagcgatcac gtggatgctc ttctgggtgc aggtgtgagt gccttgaaaa
     3421 tagaaggcag aaagaaagat gcgcaatacg tagcttcttc ggtcggtctt tataaacagg
     3481 cgcgcgatcg agctcttggc gaggcttccg gcgctgcacg caatttcagc gaagatttaa
     3541 aatcaagcta ccagagagac accacgagct tctttgtcaa aggacgatac cacgaaaacg
     3601 taatcgatct ggataacgcg ggtcatcgcg gaaccaggat tggtgtggtt cagagtgcag
     3661 gcaaaggctg gatggaagtg accacgagcg aaccattgga cctttttgat ggcttaagag
     3721 tggattcacc ggatcaggtt tatcacgccc tgccgcagca gggacagaac ttgcgtggat
     3781 ctgtaaaaaa ggcgcaggct aaatatgaaa atgaaatatg ccagttttca ttgcgcgaaa
     3841 tgcagaaagg atcgaagaga atgaccacag ctctcgcggg agatcatctt cgcatccaga
     3901 ttccatcaga tgcgcgtgtc aaagaaggag acgttgtatt tcgagtgcgc tcgaatgagc
     3961 tcaaaagata cgtggattcc atttcttctg aatcaggacg attgcgatct ctctatctca
     4021 tgcgcatgct cgtttctatt caggacggtc ctccgctgca gcttcgggcc tccctgttcg
     4081 atcattcagg agagatcgtt tctgcttcta tacagacaga agcaagtcgg cccaggggca
     4141 cttctaccct ggcgcaggac ctgcgggaaa cctttgcact gttaggtgat gcaggagttg
     4201 agtgcgatgt ggaggtcgag ggggatccag aatggtttgt tccgcgcagc aaaataaaag
     4261 aattgaagcg aatgctttct tcgcaggccc tccagagcat gcccgcgatt ctcgccgcgc
     4321 gaaagagaga tgcgaggcct tccagtcaag ggagcgctcc ggtggctcgc gcgacaacat
     4381 atcagatcaa ggtggatcgc ctggaatatt tgtccgctct ggatcaaatt ctaaccagtc
     4441 atcctggtct ggtggatgaa atcatcttcg agccaaagaa atcgaatatt tcaaaatctt
     4501 cgccgacaga tgtcttcgca tctcttcgaa gcgtagcaga caagcatagc gttcatgttc
     4561 gatttgccat tccggttgtg attcgatcgt gggatgaagg ccatctgcgc gcctatgtga
     4621 atgaatatgt tctccaggga gggtctcgat ttgaagtcgc caatgtgggt gcgtttaagt
     4681 tcctccagga tcacaagata gcgctgaccg atcttgcctc cgacttcact ctgtacgctc
     4741 tcaatagcca atcggttctt tccttgcatg agcttgggtg caatcgagtg gcgctctctg
     4801 tcgaggatga ctggcagaac ctgcagcgcc ttctagcgca atggccccac caggagatac
     4861 tccctcaggc gattttgtac aaagacacac ccctttttat tgcagaggct tgctcgctta
     4921 cggctcttca caacggttgc cctacttcga aagtctgcgg gtaccgcact ctcgagattg
     4981 aaaacgacga gggagatcgc ttctttgtag cgcatgaaca gtgcaaatcc attgtttttg
     5041 gaaagaatgc gtatagcatt tccggcagac gctccttgct cgaaggggcc ggtgtccatc
     5101 gcttcaggtt ggattttttg acccggctct attccaaaga agacatactc aggattgcaa
     5161 atctagcatt caaggaccag cagattgcaa acacccatcc agcaaatttt caccgcaaat
     5221 tgctttgacc ctggaaaaga atttgtaatt ccaaatagaa cgttgtgaat ctgctcatgc
     5281 gtattttcat tttgctttct ctgctgtgga tgattggatg caaagacctt cgcgatggtt
     5341 acgagaaagg cttcaaagaa gaattccaga agagcttcac aaaatcctgt gcgtcaagcg
     5401 cggtgaagaa gggaatgaac gaagctaagg caaccgaata ctgtgcatgc accgccaaag
     5461 aactcgccag tcggcacagt tcgcaggaat tgatgaagat ggctgcctct gacaaggacg
     5521 gagaagtggc ggcggctgtt aaggcctgta cgaagtagcg ccgtatttgc attttgaaat
     5581 gagcttgcat tgaccgcaga gtggtctggt gggcttgcaa accagagcgc agtagtccag
     5641 caccgccagg ttcaaatcgc gcgagtgttt tgtttgtacc agagaatgcg ccacatcgga
     5701 tacaaatcga tttcttctgg attcttgcgt gaactttttg ccagagatgc ggcaaatcag
     5761 gcgtgccata tttgagtcga gagcaggtag tctttcaccg taaagccggt tgcgaatcat
     5821 tgcatttgaa tattcaccta cacctggaat cctcgtgaga tcgtcgttcg cggaaggctt
     5881 tcgcgttttg tatgtatcct ttaagtaatc gatggtctca agaatctgtt tcgttctcca
     5941 tttcagacca agcggagcga gaatagcgag aacttcttcc ctgtttgctt gagctgattg
     6001 ggccgttgtg aatctggaag tgaacttagc atacactctg gctgcctggt ctgcgcgcgt
     6061 acggcgcagc atcatttctg ccatggtgag atggaaccag ttcccagttt ttctccaggg
     6121 atagtctctg gcgtttttct tgaaccaggc caggagagta cggcggacgg atctatacgg
     6181 aaattcggat gcgtccatgg gcggaagttc gctgcgtttt tgtcgcggcg tccagcgcct
     6241 ttttcatgtt ttcagcaatg gcctggacca cggggactgc cactgagttt ccgaattgtt
     6301 tgtaggcctg ggtgtccgag actacaatct tgaaatggtc agggaaaccc atgagtctgg
     6361 cacattctct gggagtgagt ctcctgggat tttttcctcg cgaaggaatc agaatttcag
     6421 atccgtcttt gtagtaacgt gcagatagag ttcttgaaat cccatcgcgc gacgccaggc
     6481 caaatccgaa accgtgtccc tgcgcttcgt gcttcgcttt gtagtcttgc aggtattgcc
     6541 agaggtgatc ggacagagtg tatttggccg gtacgttttt ctccagaatg tctgatagcc
     6601 ttggggaccg atccttcagt tccggaaatt gaaatgcttg aaatgattgc agattgcgaa
     6661 acccggcgat gaagattctt tctcgatttt gcggaagtag ttttcgagcg ttgagaaggg
     6721 agtgatgcac atggtagcca gcctctttca gtgcggccag aatcacttgc atcgttcttc
     6781 ctttgtcatg cgacgtcagg ttctttacat tttctagcag aagagcggac ggcttcttgt
     6841 ctttgaggat gcgcaatact tcaaagaaga gagtgccctg cgtttcatcg gcgaagccat
     6901 gcggtctgcc cagggcattc tttttcgaaa cccctgcaat ggaaaagggc tggcatggaa
     6961 acccaccgca gagcacatca aagtccggca aagttctgct atctactgcg cgaatgtccc
     7021 ctgccggcgt gtccttaaaa ttggctgcgt aagtgaatgc ggctgatttg tcccattcac
     7081 tcgaaaaaac agacgagtag cctgcgtttt caaacgccag acggattccg ccgatgcccg
     7141 caaacagatc gatgattttg tagtttcttc tattgttagc gcgcgcaatg ccgcgggatt
     7201 gtggccgcat agatgtatga tcggtagaaa cggacctgga gcctggaact ttaggtttct
     7261 gcatgcttct gtctacagcc tgagtgtgac aggtttcgag aaatcacgag atcgaccatg
     7321 tttccaggtg ttctagtgca gagcccggtg gcacatctgt aagaggagaa agcgactcta
     7381 actcgatgag gttttcgttt gtgtaacact gtgccacaca tcccaggtcc gggtagctgc
     7441 cgtgtggcgt gcggaattcc ttggagaatg tttttccctg acgattgtat gtgagcctgc
     7501 aggaatcacc ggttcgcgaa actcccatct tcagagcgct cgacggtttg tg
//
LOCUS       DAOQJL010000135         7546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5342_length_7546_cov_4.946469, whole genome shotgun sequence.
ACCESSION   DAOQJL010000135 DAOQJL010000000
VERSION     DAOQJL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_7546_cov_4.946469"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLG78_07795"
     CDS             complement(<1..195)
                     /locus_tag="PLG78_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23054.1"
                     /translation="MPDTAVRSVNNQQELLQQIDEITRAICQIFHDVPDEYLYSAANP
                     DGWTVEKNMKHIASTNRLMSR"
     gene            complement(245..514)
                     /locus_tag="PLG78_07800"
     CDS             complement(245..514)
                     /locus_tag="PLG78_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23055.1"
                     /translation="MPNGTKRKRSISPALDKLIGQMEDLSTDAVDAEVCKRFRLLLET
                     NEINVNADVLKKFTANPQDTDLSVFGDDIRPFLRHFRFMHKRQLP"
     gene            727..2250
                     /locus_tag="PLG78_07805"
     CDS             727..2250
                     /locus_tag="PLG78_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23056.1"
                     /translation="MRIRYALAVFVAAILAGCRYDSSCYDLDIICHPWLGLAFYRPLT
                     NPCQYQGGTSRSFYTFLGSAGFGDQARAVCAISDTSILVGGIASGNIPLLQGVTPLNA
                     YTGADDFVIVRLDTAGNVYWYTFVGSAGGTDQLTALTQTLDGGFFAVGSVTANIASLQ
                     GKTPLNAYSASTDILAIKFDQFGNVVWYTFLGGPGIETPTSAAVTIDGGLVINGSGDT
                     NIASLGGQTPLNGYNSLTDVLVMKLSSTGALSWYTFFGSAGTDAGTAVASAPDGSLLI
                     AGNASANVATLQGKTPNNAYVGADDYMIIKLSASGAVSWYTFLGGAGTDIPTGVAVMP
                     DLGAAVTGRANAAVASMQGVTPGAAYAGNFDHMVVRLTPAGNVSWFNFIGTAAASDQS
                     GSVAVYSDSSVLVSGFVAASVPSLQGRSPNNPYTAGDDFLLARYTDTGSLTWYTHLGG
                     AGNDQALSGLTPTSDGGFIVSGFSAATIASLQGKTPVNAFAGVSDMVVIKVKPDGNL"
     gene            complement(2273..4534)
                     /locus_tag="PLG78_07810"
     CDS             complement(2273..4534)
                     /locus_tag="PLG78_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE23057.1"
                     /translation="MTTLFETSLNQSPAAVMIVDIHGNIQYVNAAFSRLCGYSSEEVI
                     GKNPRFLKSGDMPKGVYQKLWETLLAGSTWTGEFHNRRKDGTTYWESASVTPVRDDSG
                     SITHFIAFKNEITKHSEDDSREAANRLRMTFNTMSEGVALNEIIFDDHGDMIDYRIID
                     VNAAFYNTADFTGKNVIGNTATALYGMSTESIRAFWQSHKSATTVSHTEMVSPLHQRH
                     FLISVSPFAGNRFVTSFLDITERKRIEAEHTRALELLRTIADNVPAMIAYWTRDLRCA
                     FANRAYEGWFGRKPDEMIGSSLDQILGPELYAKNKPYVERALAGEPQQFERTLIKPNG
                     EIGYTWAQYVPHVHDGEVQGMIVLISDITEPRKAESRKRELEAELVQARKLESLGTLA
                     GGVAHDFNNILGIILGHASLLKMSVKEPELTERLSTIENAAMRGGALVNQLLTFARKS
                     ETSLSLVSVNDTVRETVKLLSATFPRNIEISVQLSENLPSVTGNATQIQQALMNLCTN
                     ARDAMPDGGNLTISAKIADPSIIAAEFPKAHFSDFLLIEVKDTGVGMDTETKRRIFEP
                     FFTTKGPGRGTGLGLSVAYSILKEHRGLFVVDSEPGQGSTFRIYLPIESNTTTATGLT
                     IETDRSGKDTILIIEDEQMLRASLEETLVTQGYKVFTAVNGEQGVEVFQQHKDDITVV
                     ICDLGLPGLSGHEVIKKIHANRPGTRIIATSGFMDSEALQKLNQSGNPHILHKPYRLA
                     ELISAVRSEVNGI"
     gene            4782..5387
                     /locus_tag="PLG78_07815"
     CDS             4782..5387
                     /locus_tag="PLG78_07815"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE23058.1"
                     /translation="MPTPEKGILRALVRMSEIRDPYTAGHERRVAHLATAIGTRMRIG
                     EKSLHAVHLAGIVHDIGKIAVPVEILNKPGKLNSHEMGLIRTHPEVGFDILKDSDLPD
                     HVAEIVLQHHERVDGTGYPHGTRNGDIRLESRILAVADVVESMISHRPYRPGLGVEHA
                     LGEIESGIGTRYDGEVVRVCIYLFRDGGFTLPEHGSSSSQQ"
     gene            5495..7504
                     /locus_tag="PLG78_07820"
     CDS             5495..7504
                     /locus_tag="PLG78_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23059.1"
                     /translation="MLKWVSFVLLSCFSIGLFETHALHSQAQESAQDAANRIAATILS
                     GTNEEQSRAAIREAFAWAGMSTRNSGTVVLGARLPDMPIYLDESEIEILLADAKGRKD
                     GLFRMTLAELGESVNAPESVFPTDPPIPPGTVFRLSILMAVRAARQDPNNVHSFVPLF
                     LDAMAQRSYRGRDITNSRIPGNQFGLSLLEVQLLLSALMRTARPTQSYPVERDLPGQR
                     GQNVCSDLLDRTGDFNTPLRIAIRSAADVPFQSFFKFVGDNSDPAAGAFLRNSSSVLK
                     YAKMTGLMDKVMGVKYDFALNPIPAEQHYNVGSQTNVRASFKASVTLNQRLTNRDNAA
                     LDCARLLGITVPDTTEAMRRAAGGFRVYWFGVAGFPTYAEIMDGESRFNYHAPLGSDL
                     TLSGNTAFATMIVRPATESQQAHNAGLQKRESVSVVAQLDKSDANLIQNLINAVRSGV
                     GPQLAIDPAVNLMKKLFGQKREATLTVTHHQPFRLAGTIRGKFAFPRQGAGPAHELKG
                     SEELEVFFRENGTVAASYKLDWGYADMRCKNRTSNYIWTVKDSGKQNLAISDFQVQVD
                     EGGNYTIRFPLNEFFQDIGPWHPLDPMCKPGPLDYANLRGFGSPDQLITLRGTIPEGS
                     RTMSGNLTQTDAEFLNIGFSNGSSSVRAPTGARTLQWNLRITDAP"
BASE COUNT         1690 a         1873 c         1987 g         1996 t
ORIGIN      
        1 gcgactcatc aaacgattgg tgctcgcaat gtgcttcatg tttttttcaa cggtccatcc
       61 atctggattc gcggcgctat acaggtactc gtctggcaca tcatgaaaga tctgacagat
      121 ggcccgagtg atttcgtcga tttgttgcag gagctcctgc tggttattta cactgcgtac
      181 ggctgtatct ggcatacgtt caatttgatt cgatttgagc ggacgaaaag cctttttttg
      241 acgatcaggg gagctggcgt ttgtgcatga agcgaaaatg gcggagaaac gggcgaatgt
      301 cgtctccaaa aacggacaga tccgtatcct gagggttcgc tgtgaatttc ttgagcacgt
      361 ccgcgtttac gttgatttcg tttgtttcta gaagcagtct gaatcgttta caaacttcag
      421 cgtctacagc gtctgtagac aagtcttcca tctgtccgat caatttgtcc agggctggcg
      481 aaatactacg ctttcttttg gtaccatttg gcattatgtg caggaaaaaa aaatcgctcc
      541 cggggtcaat tactacgaat tgcccgatcc tcgcggcgct gcatcgcatg gacgatcttt
      601 tctgatagtg ggctagcagg tttttgcagc ttcctatgat ttttcttatg cccgaaatgg
      661 aatcctgagc tcccgggcgt tataaagaca atttgactgc ctggacctgg agactaaaat
      721 agttagatgc gcatacgtta cgcattagct gtgtttgtag cggcaatact cgccggatgc
      781 aggtacgatt cgagctgcta cgatcttgac ataatttgcc acccctggct gggtcttgcc
      841 ttctacagac cactgaccaa tccatgtcag taccagggag gaacttcgcg ctcgttttac
      901 acgttcctgg gaagcgcggg ttttggagat caggcgcgcg ccgtgtgcgc aatttcagac
      961 accagtatcc tggttggcgg aattgcaagc ggcaacattc cactgcttca gggcgttact
     1021 cccttgaatg cgtacaccgg tgcggacgac tttgtcattg ttaggttaga tacagccggc
     1081 aatgtttact ggtacacgtt tgtcggcagc gcgggtggaa cagatcagtt gactgcgctg
     1141 actcagacgc tggacggggg cttttttgca gtcggatcgg tcactgcgaa tatcgcatcg
     1201 cttcaaggga aaacacctct caacgcttat tccgccagta cagacatact cgcaatcaag
     1261 ttcgatcaat ttgggaatgt agtttggtat acatttctcg gtggtcccgg gatcgaaacg
     1321 cccaccagcg cggccgttac gatagatggg ggccttgtga tcaatggcag cggagataca
     1381 aacatcgcct ctctgggcgg acaaacgcca ttgaatggtt acaacagttt gacggatgtt
     1441 ctcgttatga agctttcctc taccggggcg ttgtcctggt acacgttctt tggtagcgcg
     1501 ggcacggacg caggcactgc cgtggcatct gcgccggacg gctcgctttt gattgcgggc
     1561 aacgcttctg caaatgtggc cactctgcag gggaaaacac ctaacaacgc gtatgtgggc
     1621 gcagatgatt acatgattat taaactctcc gcgagcggag ctgtcagctg gtatacattt
     1681 ctgggtggtg cgggtacgga cattcctacg ggtgttgctg tgatgccaga tctgggcgcc
     1741 gcagtgactg gtcgcgcgaa cgccgctgtg gcgtccatgc agggtgtgac tcccggggcc
     1801 gcatacgcgg gcaactttga tcatatggtc gtacgcctca cgccggccgg gaatgtatcc
     1861 tggttcaatt tcattggaac agcggccgcg agtgaccaat ccggatcagt agcagtctac
     1921 agtgacagca gcgtgctcgt aagtggtttc gtggccgcat cggttccgag tcttcaaggc
     1981 cgttcgccta ataacccgta caccgccggt gacgatttcc ttctggcccg ctatacagac
     2041 acaggatctc tcacgtggta cactcatctc ggcggggcag ggaatgatca ggcactctcc
     2101 gggctcactc caacatcaga tggagggttc atcgtgtcgg gcttttccgc ggcaacgatt
     2161 gcttccctgc aaggcaagac acccgtcaac gccttcgcgg gtgtaagcga catggttgtg
     2221 atcaaagtga agccagacgg gaatttgtaa atttccggca tgtctgtacg ggtcaaatgc
     2281 cgttgacctc tgatcgcacg gcagagatca attcagcaag tcgatacggt ttgtgcagga
     2341 tatgcggatt tccggattgg tttagtttct gcaacgcctc cgaatccata aacccgctcg
     2401 tcgcaatgat acgagtgcct ggccgattcg catgaatctt cttgatcact tcatggcccg
     2461 agagccccgg caggcccagg tcgcagatga ccactgtaat gtcgtcctta tgctgctgga
     2521 atacttcgac tccttgttcc ccgttcactg cggtgaagac cttgtatcct tgcgttacga
     2581 gagtttcttc cagagacgcg cgcagcatct gttcatcttc aatgatgaga attgtgtctt
     2641 tcccggatct gtccgtttct attgtcaatc cggtcgcggt cgttgtattg ctttcaattg
     2701 gcaggtaaat gcgaaatgtc gaaccctgtc ctggctcact gtcgactaca aacagtccac
     2761 gatgctcttt caggatggaa tatgcgactg acaatcccag accggttcca cgtccgggtc
     2821 ctttcgtggt gaagaacggt tcaaagatcc ttctctttgt ttctgtatcc atgcccacgc
     2881 ctgtgtcttt cacttctatc aaaagaaaat cagagaagtg agctttcgga aattcagcgg
     2941 caatgataga agggtccgca atcttcgccg aaatggtcag gttgcctccg tccggcattg
     3001 catcgcgcgc attcgtgcag aggttcatga gcgcctgctg gatttgtgta gcgttccccg
     3061 ttacagaagg aaggttttcg gaaagttgaa ccgatatctc tatattgcgt gggaaggttg
     3121 cactcagtag tttcacggtt tcgcgcaccg tatcattcac agatactaga gacagacttg
     3181 tttcgctttt gcgtgcgaat gtaagaagct gatttaccag cgcgccaccc ctcatggccg
     3241 cattttcaat cgtgctcagt ctctccgtca gctctggttc tttcacgctc atcttcaaaa
     3301 gagatgcatg tcccagaatg atccccagga tattgttgaa gtcgtgcgcg acgccacctg
     3361 ccagagttcc aaggctttcc agctttcgcg cctgaacgag ttcggcttct agttctctct
     3421 tacggctttc cgcttttctg ggttcagtga tatccgaaat caagacaatc attccctgca
     3481 cttcgccgtc gtgtacgtgt ggaacatact gggcccaggt ataaccgatt tcgccattcg
     3541 gcttgatgag tgtacgttca aattgctgtg gttcacccgc cagcgctctt tcaacgtagg
     3601 gcttgttttt cgcatacaat tccgggccca ggatctggtc cagggacgag ccaatcattt
     3661 cgtcgggttt tctgccaaac cagccttcgt atgccctatt tgcgaaggca cacctgagat
     3721 cccgggtcca gtaggcgatc atagctggaa cattgtcagc aatggttctc agtagctcga
     3781 gtgcgcgtgt atgttctgct tcaattcgtt ttctttcagt aatgtccagg aaagaggtaa
     3841 caaaacgatt tccagcaaac ggagaaacag aaatcaggaa gtgtctttga tggagggggc
     3901 tcaccatctc tgtgtgtgaa actgtcgttg ccgatttgtg actttgccag aaggctcgga
     3961 tggattcagt ggacattccg tacaatgctg tggcagtatt gccaattaca tttttgccgg
     4021 tgaagtcagc agtattgtag aaagcagcgt tcacatctat gatgcgatag tctatcatat
     4081 ctccgtggtc atcgaagatg atttcgttca gggcaactcc ttccgacatt gtgttgaagg
     4141 tcatgcgcaa tcgatttgcg gcttcgcgcg aatcgtcttc gctgtgtttg gtgatctcgt
     4201 tcttgaatgc gatgaaatga gtgatgcttc cgctgtcatc gcgaaccggt gtgacagacg
     4261 cagactccca gtaagtggtt ccgtcttttc ttcgattgtg gaattctccg gtccacgtgg
     4321 accctgcgag aagtgtctcc cagagttttt gatatactcc ttttggcata tccccagatt
     4381 tcaagaatcg tgggttcttt ccgatgactt cctcgcttga atagccgcac aggcgactga
     4441 aggctgcatt tacatactga atatttccgt ggatatccac gatcatgacc gcagcagggc
     4501 tctggttgag cgaagtttcg aagagtgtag tcacggtcat aggttggcag ctagattgtc
     4561 acaatcgagt tgcctcgtgg acgagcgatc cgcacgtttc ttcttctttc tgaatgttct
     4621 cgtcgaagtt gtctacttca aatcgcgcgc cgcattccac tctatgaaga tcttatgaga
     4681 actgatctca aagtattgtt tttttcgccc tgcaaaaatg attcatcaac cctggacagt
     4741 atttccatcg acaaggaacc cttgtgcgac atattcgccg ggtgccgacg ccggaaaaag
     4801 ggattcttcg cgcactcgtg cgaatgagtg agatacgtga tccgtataca gcgggtcatg
     4861 agcgtcgcgt ggcacatctt gccacagcta ttggaacacg gatgcggatt ggtgagaagt
     4921 ctttgcacgc agttcatctg gctggcatcg tccacgacat tgggaagatt gcggttcctg
     4981 ttgaaatctt aaacaaaccg ggcaagctca attcgcatga aatggggctg attcgcacgc
     5041 atccagaagt tggttttgat attctcaaag attcagatct tcctgaccat gtggcagaaa
     5101 ttgtattgca gcatcacgaa cgagtggatg gcaccggata tccgcacggg accagaaacg
     5161 gagacatccg tctggaaagc agaatccttg ccgtcgcgga cgttgtggag tccatgattt
     5221 cgcatagacc ttacagaccg gggcttgggg tagaacatgc cctcggtgag attgaatcag
     5281 gaatcggcac tcgatatgac ggcgaagtgg tgcgcgtatg catttatcta tttcgcgatg
     5341 gcggattcac tctgccagaa cacggatcct cttcatcgca gcaatgacac tgcgatgatt
     5401 cagcctgtaa aatgacaaag atcatactga acagtctgag ctcttgattc gtttccactt
     5461 gattttcgat tcgcaagaac aagaacattc actcatgttg aagtgggtgt cctttgttct
     5521 tctgtcctgt ttttctattg ggttgttcga aacgcatgct ctacattcgc aggcccagga
     5581 gagcgcccag gatgcagcaa atcgaattgc cgcgactatt ctctcaggca caaacgagga
     5641 acaatcgcgc gccgccattc gcgaagcgtt cgcatgggcc gggatgtcca cgcgcaatag
     5701 cgggactgta gtgctcggtg cgcgtttgcc ggacatgccc atctatctgg atgaaagcga
     5761 aattgaaatc ctactcgcgg atgcgaaggg cagaaaggac ggactgtttc gcatgactct
     5821 cgcagaactc ggggagagtg tgaacgcacc agaatctgtt tttccaacgg atcctcctat
     5881 tcctcctggc actgtgtttc gtttgagcat tctaatggca gtgcgagccg cacgtcagga
     5941 tccaaataac gttcatagtt ttgttccgct ttttctcgat gccatggccc agcgatcgta
     6001 ccggggacgc gatattacaa attcgcggat tccgggtaac cagttcggat tgagtctgct
     6061 tgaggttcaa ttgcttcttt ccgctctgat gcgcactgcg agacccacgc aatcgtatcc
     6121 cgtggaacgc gatttgccgg gtcagcgagg tcaaaacgtt tgctcggatc ttctcgatcg
     6181 cacgggagac ttcaatactc cgttgcgaat cgcgattcga tctgcggctg acgttccatt
     6241 tcaaagtttt ttcaagtttg taggggataa cagcgacccg gctgcaggtg catttcttcg
     6301 caatagttct tcggtgttga agtacgcaaa gatgacgggt ctaatggaca aagtaatggg
     6361 ggtgaagtac gattttgctt tgaatcccat tccggcagag caacactaca atgtaggatc
     6421 tcaaaccaat gtccgtgcgt ctttcaaagc ttctgttaca cttaatcaga gattaaccaa
     6481 ccgtgacaat gcggccctgg attgtgcccg tcttctcggg attactgttc ctgatactac
     6541 ggaagcgatg cgtcgcgccg ccggcggttt tcgggtttac tggttcggag tagcgggctt
     6601 tcctacgtat gcggaaatta tggatggaga aagtagattt aactatcacg ctccgctcgg
     6661 aagcgatcta acgttaagcg gcaatacagc gtttgccaca atgatcgttc ggcctgcaac
     6721 tgaaagccag caggcccaca acgcagggct gcaaaagcga gagagcgttt ctgtcgtggc
     6781 gcaattggat aagagcgatg caaatctcat tcagaatctc atcaatgcgg ttcgttccgg
     6841 ggttggtccg caactggcca tcgacccggc cgtcaatctc atgaaaaaac tgtttggtca
     6901 gaagagagag gcaactctta ccgtgacaca ccatcaaccg tttcgtttag cgggaacgat
     6961 tcgcgggaag ttcgcttttc cgcgccaggg tgcaggcccg gcgcatgagt tgaagggcag
     7021 cgaagagctc gaggtcttct tccgcgaaaa cggtactgtg gcagcgagtt acaagcttga
     7081 ctggggctac gcagacatga ggtgcaagaa ccgcacgtcc aactatatct ggacggtgaa
     7141 ggattcagga aaacagaatc tggctatttc ggactttcag gtccaggtag atgaaggcgg
     7201 aaattacaca atccgctttc ctctcaatga gttctttcaa gacattggac cctggcatcc
     7261 gctcgatcct atgtgcaaac ccggtccact ggattatgcg aatttgcgcg gatttggctc
     7321 cccggatcag ctcatcacat tgagaggaac aattccagaa ggatcgcgta caatgagcgg
     7381 caatcttaca cagacagatg cggagtttct caatattgga ttttccaacg ggagtagttc
     7441 tgtgcgagct cccactggag cgcgaactct gcagtggaat ctgaggatca cggacgcgcc
     7501 ctgaaaggca atcggattgt attctggctg ctctctgatg ttctgg
//
LOCUS       DAOQJL010000136         7542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5348_length_7542_cov_4.474957, whole genome shotgun sequence.
ACCESSION   DAOQJL010000136 DAOQJL010000000
VERSION     DAOQJL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5348_length_7542_cov_4.474957"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PLG78_07825"
     CDS             complement(<1..962)
                     /locus_tag="PLG78_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNE23060.1"
                     /translation="MCRADLALIFFYRGCTHVVSTATATAVWGQGSAGTSFTSSATGL
                     SSTTINGPTYAYPDMANGLLVIDDVNVRILYFPTNSFTATQVWGLGPPGNNFTSSGAG
                     GTTSTTFDTATALLRDAAGGLYVADSLNNRVLYFPSSSYVATKVWGQGAAGNDFVSST
                     SALSATALNGPGGLALDSTGGLYVSDTGNGRVVYFPPGSYTATRAWGINASGTTLTSG
                     GSGVSAVGLTSPKGIRVDLYGGLYVTDQARIVYYAPGSFTATKVWGQGSGGNSFTTNS
                     ASVSPTGLTNALDLALDDLGGLYAVDATANRVIYFPPQSFTATTV"
     gene            complement(1111..2196)
                     /locus_tag="PLG78_07830"
     CDS             complement(1111..2196)
                     /locus_tag="PLG78_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE23061.1"
                     /translation="MRIPAWIGRITLAVGFTLFYIVIYFGDKLTFAGGPLQTTMQSAV
                     YSLLDGLAFLVRGRPSDQWFFYGTVYTAAVLVFGVRSLIKYRASRYQMIRTGSVMFFQ
                     LFFAYLIPGWLQLFKQPEFYFSYFWPLKPEYFYPYRWAGLAASSPSFWFLFFALFMTV
                     VATPLLTYFFGKRWYCSWVCGCGALAETMGDPFRDLSNKSTAVWKFERWIVHGILAFI
                     TITTVLLWINSATSGAVFGNLSSRLAEWYGFYIGAIFSGVIGVGFYPLLGSRVWCRFG
                     CPMAAYLGILQKYFSRFRITTNGGQCISCGNCSNYCEMGIDVKWYAQRGQDIVRASCV
                     GCGICSEVCPRGVLKLEAVSGARANRH"
     gene            complement(2193..3473)
                     /locus_tag="PLG78_07835"
     CDS             complement(2193..3473)
                     /locus_tag="PLG78_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE23062.1"
                     /translation="MNLVIAGNGITGLTAARTVRKVLPRAKIQIVSNESDYPYARTAL
                     MYIFMGHLKERATECYERKFYKDNSLELIRDTALRADTKACTVYLESGRTLPYDALLI
                     ATGSTPSLYDWPGQDLEGVQGLYSLQDLQKLEENARSAHHAVIVGGGLIGVELAEMLH
                     SRKIPVSMLVRENSYASHILPPEESELVNFEIRKHRIALHLGESLKEITGTTRASGVT
                     TVSGREIKCNLVGLTAGVSPNTGFAKASSIDCARGVLVSDLFQTNAPGVFAAGDCAQF
                     KEPGGPGRVEQLWYTGRIQGELAGLQICKMLSGQTPGAPYQPGVWFNSARFFTLDYQA
                     YGTMPKDSKSVLMIDHANQKSIRIACHGATRAVCGFVLMGIRYRHEVCEKWIQEERSL
                     DYVLANLHEANFDPEFSKKYEPLVRTQAEKAGFI"
     gene            3553..3888
                     /locus_tag="PLG78_07840"
     CDS             3553..3888
                     /locus_tag="PLG78_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNE23063.1"
                     /translation="MEETYYNPADLAKFGSISDLQPDLGKAFFAYYGKVFEAGALTQR
                     EKSLIALAVAHAVQCPYCIDAYTTDCLEKGSDETQMMEAIHVAAAIRGGASLVHGVQM
                     MNHAKKIGM"
     gene            3888..4919
                     /gene="arsS"
                     /locus_tag="PLG78_07845"
     CDS             3888..4919
                     /gene="arsS"
                     /locus_tag="PLG78_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671343.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNE23064.1"
                     /translation="MAGTLLRANHPLALPEAQRKFLESGSAPAFDSVLLKQGAGVLTS
                     SGIDILQMNVGRVCNQTCAHCHVDAGPDRRESMSRGTFEHCLRVLEEGNIPVVDITGG
                     APELNENFRWFVESASGLGKKVMVRCNLTIILSNERFMDLPEFFARHRVQVISSLPHY
                     SETRTDAQRGAGVFERSMKALKLLNDAGYGREDSGLELHLVYNPVGAFLPGSQSELED
                     QFKFQLLSRYAITFNHLYCITNMPISRYLEYLVRSGNYEAYMNRLVSAFNPAALPGLM
                     CRNTLSVGWDGRIFDCDFNQMLDLEAKPSSHISTLDLAALRRREIVTGRHCYGCTAGA
                     GSSCGGQTA"
     gene            5090..>7542
                     /locus_tag="PLG78_07850"
     CDS             5090..>7542
                     /locus_tag="PLG78_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE23065.1"
                     /translation="MRYLKLYLLPFFSFALIAQCVGRSGAEDYVLFGVTIRQGTDVSP
                     AASCASEIAVTTTQMDLVEDGDVTRSYSTTSPFYTDVDSDGGTSWGFKNMETCVYLVN
                     AFNGTVEIPITTNSTYSGRLNVKTSFPVAASGNWPGSNQNLPAKLVFTGNGTTGHGAT
                     SRQCFTMAVIQNVAQNPKESPFTVSLGEITSADDAGIYKGKNPCDVSASVEDDEGPGV
                     RVSSISRVMEEPGGAGFTNAQFLVRLRTMPSSSVVIPINDTYDSTNSSHREGIASPTS
                     LTFTTGNWNVDQAVTVTSQDDLEVDGTKTYTVQVAAASSGDSSYNGFKPRDVIVINND
                     KSVPGYTYTLFDTSTGNTNSSTGATVNGFATDEMNNMSWTYSNFNIKLRSKPSSNVTL
                     NFSTSNSAISNLITTSLTFTPANWNVDQTVYVTGKSNGTDGPSGNGNLDYTISFTTTT
                     ADSTYSSIPKPKFVMRSCDNDNTHLIQPCNFSGQPYGDSRGRLSGAEPSSTTNMWLIT
                     KSSPGSTVTVGLTSTDTTEGTVPANVTIDSTNYNALGGGSNKIVLSHVDDTALDGTIT
                     WDVTTALSTGGLSYNTADVNAQTTDNEQRYYINVTGTTKENTPGQTATIDVCLGATNA
                     NSVTINAACVGTAECGSVSPANVTFTPGQTISGANPTNASCSSDANKKTFTVTGADDS
                     YADGTQSYNVSLTVTTTDPIYTGNDPGNQGVSNQDDELPGHAVFVYSTAVVGEMTLSG
                     IGGADNYCANNKPAYAPAGTYKALMVSDDASNRRIATTDGSTSAGQVGWVLTPNNYYY
                     RCSGSGAANCSDQYTRLFIA"
BASE COUNT         1862 a         1959 c         1994 g         1727 t
ORIGIN      
        1 actgttgtcg cggtaaagct ttggggcgga aagtagatca ccctgttcgc cgtggcatcg
       61 acagcatata gaccacccag atcgtccaaa gcaagatcca gtgcgttggt gaggccggtg
      121 gggctgacgc tggcgctatt tgtagtgaag ctattcccac cggagccctg gccccagact
      181 ttcgtggcag tgaacgaacc cggagcgtag tagacgattc gggcctggtc cgtcacatac
      241 aatccgccat acaaatccac tcttatccct tttggtgagg taagtccaac tgccgagacg
      301 cccgagcctc cagaggttaa ggtggttccg gatgcattga ttccccaggc ccgtgtggca
      361 gtgtagctac ccggcgggaa ataaacgaca cggccgttcc ctgtgtcgga cacatacaat
      421 ccacccgtgg agtccagtgc cagacctccg ggcccattga gtgcagttgc cgaaagtgcg
      481 ctagtggatg agacaaagtc gtttccagcc gccccctgac cccacacctt cgttgccaca
      541 taagaacttg agggaaagta caaaacgcga ttgttgaggc tgtctgctac gtatagccct
      601 cccgccgcgt ctctgagcag ggcagtcgct gtgtcaaatg ttgtggaagt cgttcctccc
      661 gctccagatg acgtgaaatt attaccggga ggcccgagtc cccagacttg cgttgccgtg
      721 aagctgtttg tggggaaata caggatgcga acgttgacgt catctattac gagcaatcca
      781 ttggccatgt ccggatacgc gtaggtggga ccgtttatgg tcgtagaaga aagcccggtg
      841 gcactgctcg tgaaactcgt tcccgcagat ccctgacccc acactgcggt tgccgtggct
      901 gtgctgacca catgcgtgca tccacggtaa aagaagatca gagcaagatc cgcgcggcac
      961 atgatgtctg atgaagagca atgagacggg tatctgcaat gaagcaggcc gggcagcagc
     1021 agtaacagag cgtagggact aaccttcacc atgatttaga cggcagaaac tcgtttgagg
     1081 gcgagtgaat tttgaagaat tgtttccgat ctagtgccga tttgctctgg cgccagatac
     1141 agcttccagc ttcagcactc ctcgcggaca gacttctgaa cagatgccac acccaacgca
     1201 ggaagcgcgc acaatgtcct ggcctcgctg cgcataccat ttcacgtcga ttcccatttc
     1261 gcagtagttg ctgcagttgc cgcaactgat gcactgaccg ccgttggtag tgatgcgaaa
     1321 cctggagaaa tacttctgca gaatcccaag gtaagcagcc atagggcagc caaatctaca
     1381 ccagacgcgt gaaccgagaa gtggataaaa gcctactcca atgactccac tgaagatagc
     1441 accaatgtag aatccatacc attcagcgag tctcgaagaa aggtttccaa agacggctcc
     1501 ggaggttgcg gagttgatcc acaaaagaac ggttgtgatt gtgatgaagg caagaattcc
     1561 gtgcacaatc cagcgttcga atttccagac tgctgtgctc ttgttggaaa gatcgcgaaa
     1621 tggatctccc atggtttccg caagtgctcc acatccgcaa acccaggagc aataccatct
     1681 cttgccaaag aaataagtaa gaagaggtgt tgctacaaca gtcatgaaga gagcgaaaaa
     1741 tagaaaccag aacgaaggac tcgatgcggc gaggcctgcc caccggtagg gatagaagta
     1801 ttctggtttc agaggccaga aataactgaa gtagaactct ggttgcttga aaagttgcag
     1861 ccaaccaggg atcagatacg caaagaacaa ctgaaagaac atgaccgaac cggtgcgaat
     1921 catctggtaa cgcgaagccc tgtatttgat gaggctgcgc actccaaaaa ctaaaaccgc
     1981 agctgtgtaa accgttccat agaaaaacca ttgatccgaa ggccttccgc gaaccaggaa
     2041 ggcaaggccg tcgagcaggg aatagacagc agactgcatg gtcgtttgca gaggcccgcc
     2101 agcaaaagtg agtttatctc caaaataaat tactatgtaa aaaagagtaa atccaacagc
     2161 cagagtgatc cgaccgatcc atgccgggat tctcaaatga aacctgcctt ttctgcctgc
     2221 gttcgtacca ggggttcgta tttttttgaa aattcaggat cgaaattagc ttcatgcaag
     2281 tttgcaagta cgtagtccag actccgctct tcctgaatcc acttttcgca cacctcatgt
     2341 ctgtagcgga ttcccatcag aacaaatccg cacacagccc gggttgctcc gtggcaggcg
     2401 atgcgaatgg atttctgatt cgcatggtct atcatgagaa ccgatttgct gtctttgggc
     2461 atagttccgt acgcctgata gtccagagta aagaagcgcg ccgaattgaa ccacacaccg
     2521 ggctgatagg gtgccccggg cgtctgaccg gacaacattt tgcagatctg taatcctgca
     2581 agctcaccct gaatccttcc agtataccag agttgttcta ctcgtccggg tccgcccggt
     2641 tctttgaact gggcgcaatc gcctgccgcg aaaacgccag gtgcgtttgt ttgaaacagg
     2701 tcagagacaa gcactcctcg ggcgcaatct atactgcttg ctttcgcaaa gccagtgtta
     2761 ggcgacacgc ctgcggtcag tcctaccaga ttgcatttga tttcgcggcc acttacggtc
     2821 gtgacaccgg atgcccttgt tgtccccgtg atttctttga gactctcccc taaatgaagc
     2881 gcgatgcggt gcttacgtat ctcgaagttc acgagttcag attcttctgg aggcagtata
     2941 tgggaggcgt agctgttttc gcgcacgagc atggagaccg ggattttcct tgaatgaagc
     3001 atttctgcta actccactcc gatgaggcct ccgcccacga tgaccgcgtg atgcgcagac
     3061 cttgcgttct cttcgagttt ctgtagatcc tggagagagt aaagtccttg aactccttcc
     3121 aggtcttgcc cgggccaatc gtagagagaa ggggtagagc cagtggcgat caacagtgca
     3181 tcgtagggaa gggttctgcc actctcgagg tacaccgtgc aggcttttgt atccgcgcgt
     3241 agagccgtgt cccggatgag ttccagagag ttatctttgt agaactttct ttcgtagcac
     3301 tctgtcgctc gctctttcag gtgtcccatg aaaatgtaca tgagagcagt gcgagcgtat
     3361 ggatagtctg actcgttgga tacgatctga atctttgcgc gaggtagaac ctttcgcact
     3421 gtgcgcgcgg ctgtcaaacc ggtgatgccg ttgcctgcaa tgaccaggtt catgtaaaca
     3481 gatggccgga tgctttcccg gggtcaaaga aaaaagacgg gacgacgtgt ttcatggatt
     3541 tgtttattgt ccatggaaga aacgtattac aacccggcgg accttgcgaa atttggaagt
     3601 atctcggatt tacagccgga tctgggcaaa gcattctttg cttactatgg taaggtcttt
     3661 gaagcgggcg cccttacgca gcgcgagaag tctctcattg ctcttgcggt ggctcatgca
     3721 gtccagtgtc cctattgcat tgacgcatac actactgatt gtttggaaaa aggctcggat
     3781 gaaactcaaa tgatggaagc aatccacgta gccgcggcta ttcgcggagg agcatctctc
     3841 gtgcatggag tgcaaatgat gaatcacgcg aagaaaatcg gaatgtgatg gctggaactc
     3901 ttcttcgggc caatcatcct cttgcgctac cggaagcgca gcgcaaattt ctagaatcgg
     3961 gaagtgctcc cgcgtttgac tccgttctgt tgaaacaggg ggcgggcgtt ctgacctcat
     4021 ccggcattga tattctccag atgaatgttg gacgcgtctg caaccagact tgcgcgcatt
     4081 gtcatgtgga cgcagggccg gatagacgcg agagtatgtc gcgcggaacg tttgaacact
     4141 gtttgagggt gctcgaagag ggaaacatcc cggtggtgga tatcacgggt ggcgctcctg
     4201 agctaaatga aaattttcgc tggtttgtag aatccgcatc cggcctcgga aaaaaggtga
     4261 tggttcgctg caacctcacc atcattcttt ccaatgaacg tttcatggat ttgccggaat
     4321 tcttcgccag gcatcgagtc caggtcattt cctctttgcc ccactattct gaaactcgca
     4381 cggacgcaca aaggggggca ggtgtttttg agagatccat gaaagccctg aagctgctca
     4441 atgatgcggg ctacggtcgg gaggattctg gcctggagct tcatctggtg tacaatccgg
     4501 ttggcgcgtt tctgcccggc tcccagagtg agctcgaaga tcagtttaaa ttccagcttc
     4561 tttccagata cgccataacg tttaaccatc tgtattgtat aaccaatatg cccatcagcc
     4621 gctatctcga gtatctggtc cggtccggca attacgaagc ttatatgaat cggctggtga
     4681 gtgcgtttaa tccggcagcg ctgccgggtt tgatgtgcag gaacactctg tctgttggct
     4741 gggatgggcg tatatttgac tgcgatttca atcagatgct ggacctggag gcgaagccct
     4801 cttcgcatat ttctactctt gatctggccg ctcttcgcag acgcgagatc gtcaccggca
     4861 ggcactgcta cgggtgcacg gcaggcgccg ggtccagctg cggcgggcag accgcctgac
     4921 gttctcgttt ggcgcgaggt gttcgcacac tgatcgcacg tcgtgcgcac tcttcgcaca
     4981 acacgttaaa gacgccgttt gaactagtcc cttcgcagta gaaaaaaatc tctcccctcc
     5041 gcggctttgt ttctggacat tttctatgaa ttctaatcta ttgcgcccaa tgcgttattt
     5101 gaaactgtac ctgctaccat tcttctcttt cgcgctcatc gcacagtgcg tgggccgctc
     5161 tggagcggag gattacgtct tgtttggagt gaccatccga cagggcactg acgtatcgcc
     5221 tgccgcgtcc tgcgcatccg aaattgcagt gaccacaacg cagatggatt tagtagaaga
     5281 cggagacgtt acacgatctt attccactac ttctcctttt tacacagacg tagactcaga
     5341 tggcggcact tcctggggtt tcaagaacat ggaaacgtgc gtctatcttg tgaatgcttt
     5401 caatggaaca gttgagatac caatcaccac caattcaact tattccggcc gcctcaatgt
     5461 gaagacttcc tttcctgtgg cggcttctgg caactggccc gggtccaatc aaaatcttcc
     5521 ggcgaagcta gtgttcacag gaaacggcac tactggtcac ggagccactt ctcgtcagtg
     5581 tttcacaatg gcagtcatcc agaatgttgc gcagaatcca aaagaatctc ccttcactgt
     5641 gagcctgggc gaaatcacaa gcgcggatga cgcgggcatt tacaaaggga agaatccatg
     5701 cgatgtatct gcgagcgtgg aagacgacga aggcccgggc gttcgagtat ccagtatttc
     5761 tcgcgtaatg gaagagccag gtggagccgg gtttacgaat gcacaattcc tcgtccgtct
     5821 gcgcaccatg ccatcttcga gcgtagtgat tccgatcaac gacacgtacg attccacgaa
     5881 ttccagtcac agggaaggga ttgcaagtcc cacctctctt acgttcacaa caggcaactg
     5941 gaacgtggac caggccgtca ctgttacatc tcaggatgat ctggaagtgg atggcaccaa
     6001 gacatatacc gtgcaagtag cagcggcctc cagcggtgac tccagctaca acggattcaa
     6061 gccgcgcgac gtgatcgtga tcaacaacga caagagcgtt ccgggctaca catacacact
     6121 gtttgatacg agtacgggca acacgaactc atctacaggg gcaaccgtaa acggatttgc
     6181 aactgatgag atgaacaaca tgagctggac ctattctaac ttcaacatca agcttcgctc
     6241 caagccttca tcaaatgtga ctttgaattt ctcaaccagc aactctgcca tcagcaacct
     6301 gattaccacc agccttacat tcactcccgc caactggaat gtggatcaga cggtgtatgt
     6361 gaccggaaaa tcaaacggta cggatggacc gtctggaaac ggaaacctgg actacacgat
     6421 ttcgttcacc acaactacag cggacagcac ttacagttcg attccaaagc caaagtttgt
     6481 gatgagatcg tgtgacaacg acaacactca tttgatccag ccctgcaact tctccgggca
     6541 gccatacggt gattcgcgtg gcaggttgag tggggcggag ccttcatcta ccacaaacat
     6601 gtggctaatc acgaagagca gcccgggttc tactgtaacg gtcggtttga cgagcacgga
     6661 caccacggaa ggtacagtgc cggctaacgt tacgattgat tctacgaact acaacgcgct
     6721 cggcggaggt tcgaataaga tcgtgctttc gcatgtggat gacacggctc tggacggaac
     6781 cattacctgg gacgtgacca cggctctatc gaccggcggc ctcagttaca acactgcgga
     6841 cgtaaacgcg caaacgacag acaacgaaca gagatactac atcaacgtca cgggaactac
     6901 aaaggaaaac actccgggcc agactgcgac catcgatgtt tgtctcgggg cgaccaatgc
     6961 gaacagcgtg accatcaacg ctgcctgtgt cggaaccgct gagtgcggat ctgtgagtcc
     7021 tgccaatgta acgttcacgc cgggtcagac gatctctggt gcgaatccta cgaatgctag
     7081 ctgttcctcg gatgcaaaca agaagacatt cacggtaacg ggtgcagacg attcttacgc
     7141 agacggaacg caaagctaca atgttagcct cacggtgacc actacggatc cgatttacac
     7201 cggaaatgat ccaggaaacc agggagtttc caatcaggat gacgagctac caggacatgc
     7261 cgtgtttgtg tactcaactg cggtcgtggg agaaatgact ctcagtggca ttggaggcgc
     7321 ggacaattat tgtgcgaaca ataagccggc gtacgcacct gccggaactt acaaggcact
     7381 gatggtaagc gatgacgcaa gcaatcgaag aattgcaacc acggacggct ccacgagcgc
     7441 gggtcaggtg ggctgggtgt tgacaccgaa caactactat tatcgttgca gtggatcggg
     7501 cgcagcgaat tgcagcgatc agtacacgag gttgttcatt gc
//
LOCUS       DAOQJL010000137         7540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5353_length_7540_cov_4.194255, whole genome shotgun sequence.
ACCESSION   DAOQJL010000137 DAOQJL010000000
VERSION     DAOQJL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5353_length_7540_cov_4.194255"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PLG78_07855"
     CDS             complement(<1..1039)
                     /locus_tag="PLG78_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE23066.1"
                     /translation="MKRSRTGNLLPGVILAVGFGFSIGFFLISRDAVDRQVRDSFHLT
                     VSFEAAAIKDNMLMHEKELRELGAAVQGLGGGPALQRYLQGVDFGTNFPGFETIDFIS
                     TSGGLQRLYPAGERLSQNTYAHLQGELGKARAQKFLVAMASPGDDQIITWVQPLDGGD
                     LIVSKVSLARLIHALFRDRAVYTLDPGSQGVYFEVITISTGGLQKHLYFPMPADFEDT
                     AGRTLPYMILGSGSIISLLLFIVIRSLSGARDQALTLAEKMTEDFRRATSEAIRANQA
                     KSEFLASMSHEIRNPIHVITGMSEILAGTNLAEDQRDYLRSLRNAGDHLLGLINDILD
                     LSRIESGQLELE"
     gene            complement(1036..1716)
                     /locus_tag="PLG78_07860"
     CDS             complement(1036..1716)
                     /locus_tag="PLG78_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE23067.1"
                     /translation="MKRRLLLLVLLPVLCGQTPSRLSRITFARGEIFVTHAGETKPAE
                     SGQILYHDDMIRTGKDSYAEVMVQNVGVFRLAENSELKLEELQSKDKTIIRLDKGKAG
                     FFLLKIPSKEQVQVRLPTAVAGGRGTNFLVSAEKGSKVALFGGAVQMEDTAKKSVVID
                     KPGEITLKAGESLANKKVEKLSAESIADMKKLEEMLPLNLQSVPQDPGRMNQTDLDAP
                     QGSDLKKP"
     gene            1832..3232
                     /gene="leuC"
                     /locus_tag="PLG78_07865"
     CDS             1832..3232
                     /gene="leuC"
                     /locus_tag="PLG78_07865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNE23068.1"
                     /translation="MKPRTLYDKIRENHIVNEDAESVLLYIDRHLVHEVTSPQAFEGI
                     RMAGRKIRRPDLTFATMDHNVSTRSRDWGAAGELSRIQMETLSKNCKDAGIRIFDLNN
                     PDQGIVHIIGPEMGLTLPGLTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVH
                     QKSKNMLISIEGDTGPGVTPKDIILYIIGVIGTNGGQGSVIEYSGEAIRKLSMEGRMT
                     ICNMSIEAGARAGLIAPDETTFEYLKGKDFAPRGADFDKAVAYWKTLPSDEGAHYDRV
                     VKIKGSDITPMVTWGTNPGQVASVAAAVPDPDSMSDNVERETARHALKYMGLKAGQKM
                     TDITVDNVFIGSCTNGRIEDLRIAAKLAKGKKVKSGVKAIVVPGSGRVRRQAEEEGLH
                     KVFLDAGFEWRLAGCSMCLGMNDDVLAPEARCASTSNRNFEGRQGRGARTHLVSPQMA
                     VAAAVEGKFVDVRNWN"
     gene            3232..3849
                     /gene="leuD"
                     /locus_tag="PLG78_07870"
     CDS             3232..3849
                     /gene="leuD"
                     /locus_tag="PLG78_07870"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNE23069.1"
                     /translation="MQAFTKLSGVAAPLDRSNVDTDQIIPKQFLKKIERSGFGIHLFH
                     DWRFSDADGKVPIADFLLNRAPYNKASILLARENFGCGSSREHAPWALEDFGIRSIIA
                     PSFADIFYNNCFKNGMLPVILPTGQVDELFEYEAKNPGQSFEIDLPAQLIRRPDGKTY
                     SFEVDAFRKECLLNGWDDIGLSLRHADKISSFEKSYFEKFGYVKV"
     gene            3836..4216
                     /locus_tag="PLG78_07875"
     CDS             3836..4216
                     /locus_tag="PLG78_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23070.1"
                     /translation="MLKFRFFSFLLLAAVASGVFLANCGPARDPIPTGITTAEMAAGQ
                     IRVTATGRAEQGTIDRANGAMMRTTSCDAARLLLKNELKSTAYKNPSRNFKESSEVHL
                     IYNGEYCRLSGIYDPAGKFEPTTP"
     gene            4197..5039
                     /locus_tag="PLG78_07880"
     CDS             4197..5039
                     /locus_tag="PLG78_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNE23071.1"
                     /translation="MNPQLLDGKAASEQIRNDLRSRVEKLKLRGGPVPVLVTILVGNN
                     PSSEVYVKMKGKACESIGLGSRKIQMPEETTTEELLKVIDECNADPTVRGILLQHPSP
                     SQIDERAAFDRIAIDKDVDGVTAFGFGRMSLGIQAYPACTPAGIVKLLDHYKINLSGL
                     HAVIVGRSPILGKPMAQLLLNRDCTITVCHSRTKDLPRHLQSADLVVAACGKPRYIVG
                     DWIKEGAIVVDAGYNDGNIGDADFESCAKKASWITPVPGGVGPMTITTLIAHTVESAE
                     KASG"
     gene            complement(5041..5787)
                     /locus_tag="PLG78_07885"
     CDS             complement(5041..5787)
                     /locus_tag="PLG78_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE23072.1"
                     /translation="MRDNIVYGRQTVREFLRADNPPRVNKIYLSESFPNDLRSQVMAL
                     GGAVQELPRREIDRMFPGIHHQGVVLELAGSYRAAAPATAKDALALGGPFLALDEVSD
                     PQNLGSILRTAEALGVRAVFSGSKTSPVTPAVHRASSGASLHIPAYNVGNLPQFVERM
                     KEAGIWIIASVPDETEPARGQTFLRTTDSDDLPEAEKLCLLVGSEGTGIKSILIERAD
                     YLMTIPMHGKTGSLNAGVACAIILERIVNR"
     gene            complement(5784..7322)
                     /gene="cysS"
                     /locus_tag="PLG78_07890"
     CDS             complement(5784..7322)
                     /gene="cysS"
                     /locus_tag="PLG78_07890"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNE23073.1"
                     /translation="MSFRLFNSLSGKKEEFQPGQPGVVKLYNCGPTVYNFNHIGNFRS
                     YMSVDVLRRSLLFFGYKVDHTTNITDVEDKIIANAAKQGKDIFAFTKPFIEYFLEDLA
                     YLGIQDVEHRPKATDSIPAMIDLMQKLEKSGHTYSQDGDVYFRLKSFSEYGKLSRIEP
                     EQLKAAAGGRFTADEYTKEDIRDFALWKKQQAPSEPAWDSPYGKGRPGWHLECSAMIR
                     DIYGAGGVDIHAGGIDLLFPHHENEIAQSCCAYRGENFVRYWVHNEHLLVDGKKMSKS
                     LGNYFTLRDLSEKQGLEKLISENRAPADTMKLHEKLSMKRALRYLLLSTHYRSRLNFT
                     FENLKASDAACERIQTCLDKALGTSGITEKQAATTFAARDPAQPQKLFADKGLATAIQ
                     SFQDALLDDLNISRALAAVFDAVSDLNSRGMDADLARQAVVFFYAANQIIAVLDFRPE
                     DQREKPQADLGDLASYVQSKIEERLRARAEKNWALSDQIRDELKAKGIILKDSPQGTT
                     WEKA"
     gene            complement(7353..>7540)
                     /locus_tag="PLG78_07895"
     CDS             complement(7353..>7540)
                     /locus_tag="PLG78_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004771712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23074.1"
                     /translation="FRWASIAYNGGPGSLARWKAQYYHGDFNLFLENIPSKESRNYCR
                     VTFQNYMHYRTTYTLYP"
BASE COUNT         1841 a         1828 c         1936 g         1935 t
ORIGIN      
        1 cttctagctc cagttgccct gattcaatgc gcgaaagatc cagaatgtca ttgatgaggc
       61 caagaagatg atcgcccgca tttcgcaggg aacgcaaata atctctctgg tcttctgcga
      121 gatttgtgcc ggcaagaatt tcgctcatgc cagtgattac gtggattgga tttctaattt
      181 catgactcat acttgccagg aattctgatt tagcctggtt ggcgcggatt gcttcactgg
      241 tggcgcgccg gaaatcttcc gtcatttttt ctgccagagt cagagcctga tctctagcac
      301 cggagagtga gcgaatcaca atgaaaagaa gaagagagat aatcgagccg gaaccaagga
      361 tcatgtaggg aagagtgcgg cctgcagtgt cttcaaaatc tgctggcatg gggaaataca
      421 gatgcttctg caatcctcct gtgctgatgg tgatcacttc gaagtaaaca ccctgactgc
      481 ctggatccag ggtatacact gcgcgatcgc gaaagagtgc atggatcaat ctggccaaac
      541 ttactttaga aactataaga tctcctccgt ccagaggctg cacccacgta atgatctgat
      601 catctcccgg gcttgccatt gccaccagga atttctgagc gcgtgctttc cccaattcgc
      661 cttgcagatg cgcgtacgta ttttgagaga gtctttcgcc tgcagggtag agtctttgta
      721 atccgccgga agtggaaatg aaatcaatgg tctcgaatcc cggaaagttg gtgccgaagt
      781 ccactccctg aagatatctc tgcaaggctg gtcctccgcc caggccctga actgcagcac
      841 ccaattcacg cagctccttt tcatgcatca gcatgttgtc tttgatcgct gccgcctcaa
      901 agcttacagt caggtgaaag gaatcgcgca cctgtctgtc cactgcatcg cgcgaaatca
      961 agaagaagcc aatggagaat ccgaaaccaa ctgccagaat gactcctggc agcaagttcc
     1021 cggtgcgaga acgtttcacg gtttctttag atcagatccc tgcggggcgt ccaggtctgt
     1081 ctggttcatc ctccctggat cctgtggaac ggattgcaaa ttgagcggaa gcatctcttc
     1141 cagttttttc atatctgcga tggattctgc actgagcttt tccactttct tgttcgcaag
     1201 agattctcct gctttcaaag tgatctctcc gggcttgtcg atcacgacac tttttttggc
     1261 ggtgtcttcc atttgaaccg ctcctccgaa gagtgccacc ttggagcctt tttcagcaga
     1321 cacgaggaag ttggtcccgc gaccacctgc gaccgccgtt ggcaatctta cctgcacttg
     1381 ttctttcgat ggaatcttta acaggaagaa gccggcctta cctttatcca ggcgaatgat
     1441 ggttttgtct ttggattgaa gttcttccag ttttagttcg gagttttcag ccagcctgaa
     1501 tacacctacg ttttgaacca tcacctctgc gtaagaatct tttccagtgc gaatcatgtc
     1561 gtcatggtat aagatctggc cagactctgc tggttttgtt tcccctgcat gagtgacgaa
     1621 tatttctccg cgtgcaaaag taatgcggga caatctggat ggagtctgtc cgcagagaac
     1681 gggcaaaagt accagtagaa gtaggcgacg tttcataagc aaatggcctc aagcgggcgg
     1741 gtgccgtcca ttgaattcca ggcatgccca tgtaaaaaaa gacataaaat cctttctgga
     1801 atagggcctt tttcgttttc tgtacccggc gatgaaacca cgcacattat acgacaaaat
     1861 tcgtgaaaac cacattgtaa atgaggacgc agagtctgtg cttctctata tagaccggca
     1921 tctggttcac gaagtaactt ctcctcaggc tttcgaaggg attcgaatgg ccggccgtaa
     1981 gattcgcaga ccggatctaa cgttcgcaac catggatcac aacgtctcca ctcgttctag
     2041 agactggggc gctgcaggcg aactctccag aatacagatg gagactctgt ctaaaaactg
     2101 caaagacgcc gggattcgga tttttgattt gaacaatcca gatcaaggga tcgtccacat
     2161 cattggtcct gagatgggtt tgactttgcc tggcctcacc atcgtttgcg gagattctca
     2221 tacaagcacg cacggtgcat ttggtgctct tgcatttgga atcggcacaa gcgaagtaga
     2281 gcacgtactg gcaacgcaga ctctggttca tcagaagtca aagaacatgc tcatctccat
     2341 tgaaggagac actggacctg gtgtgactcc gaaagacatc atcctctaca tcattggtgt
     2401 gattggcaca aacggcggcc agggttccgt catcgaatat tcaggagaag ccatccgcaa
     2461 actttctatg gaagggcgaa tgactatctg caacatgagc attgaagcag gcgcccgcgc
     2521 gggtctcatc gctccggatg aaactacatt tgaatatctc aaaggaaaag atttcgcacc
     2581 aaggggcgcc gattttgaca aagcggtggc ctactggaag actcttccat cagacgaagg
     2641 ggcacattac gatcgagtgg tgaagatcaa aggttctgac atcacaccta tggtaacctg
     2701 gggaaccaat ccaggtcagg tcgcgtccgt tgctgcggct gtgccggatc cagactcaat
     2761 gtcggataac gtggagcgag aaacagcacg tcatgcgctc aagtacatgg gactgaaagc
     2821 tggtcagaag atgaccgata tcaccgtgga caatgtattc attggatcgt gcacgaacgg
     2881 ccgcattgag gatcttcgca ttgcagcaaa actcgcgaag ggaaagaaag tcaaaagcgg
     2941 agtcaaagca atcgtggttc ctggatctgg ccgggtgcgc agacaggcag aagaggaagg
     3001 acttcacaag gtatttctcg atgccggttt tgaatggaga ctggcaggct gttctatgtg
     3061 cctgggcatg aacgatgatg tgctggcacc ggaagcaaga tgcgcttcta cttccaacag
     3121 aaactttgaa ggcagacagg gtagaggagc acgcactcat ctggtgagtc ctcagatggc
     3181 cgtggccgcc gccgtagaag gcaaattcgt agatgtgagg aactggaact gatgcaggct
     3241 tttactaaac tatcaggcgt agccgccccg ctcgaccggt ccaacgtgga cacggaccag
     3301 atcatcccca agcaattcct aaagaaaatt gaacgttctg gatttggaat tcatctgttt
     3361 cacgactgga gattctcaga cgcagacggg aaggttccca ttgctgattt tcttttgaat
     3421 cgcgcacctt acaataaagc ctcgattctg cttgcgagag agaactttgg ctgcggttca
     3481 agcagggagc atgcaccctg ggctctcgaa gattttggaa ttcgctccat catcgctcct
     3541 tctttcgcag acatcttcta caacaactgc ttcaagaatg gaatgcttcc tgtgatcctt
     3601 ccgacgggcc aggtggacga actctttgag tatgaagcaa agaatccggg gcagtctttt
     3661 gaaattgatc tgcctgcaca gttgatccgt cgtccggacg gaaagacgta ttcctttgaa
     3721 gtggatgctt ttagaaagga atgcctgctc aacggctggg atgacattgg tttgtctttg
     3781 cggcatgcag ataagatctc atcctttgaa aagtcttact ttgagaaatt tggatatgtt
     3841 aaagtttaga ttctttagtt ttctgcttct ggccgcggtt gcctctggag tgtttctcgc
     3901 aaactgcggt cctgcgcgtg atcccatccc aaccgggatc accactgcag agatggcagc
     3961 gggccagatt cgagtaactg cgacaggacg tgcggagcag ggaacgattg atcgtgcgaa
     4021 tggtgcgatg atgcgcacaa ccagctgcga tgcggccagg cttcttctga aaaatgaatt
     4081 gaaaagtact gcatacaaaa atccgtccag gaatttcaaa gaaagtagcg aagtgcactt
     4141 gatttacaac ggcgagtatt gcagactcag cggaatctac gatccggcag gaaaatttga
     4201 acccacaact ccttgatggc aaagctgcgt cagaacaaat ccgaaatgat cttcgctctc
     4261 gcgtagaaaa gctgaagctg cgaggcggtc cagttccggt gctcgtgacc atcctcgttg
     4321 gaaacaatcc atcttcagaa gtttacgtga agatgaaagg aaaggcctgc gaatcgattg
     4381 gccttggatc gcgcaagatc cagatgccag aagagaccac tacagaagaa ctactgaaag
     4441 taatcgatga gtgcaatgcg gatccaaccg tccgcggaat attgctgcag catccttctc
     4501 cttctcaaat tgatgagcgt gcagcattcg acagaatcgc aatagacaaa gacgtagatg
     4561 gagtcactgc atttggtttt ggcagaatga gtcttggaat tcaagcttac cctgcctgca
     4621 cccccgcagg aattgtgaag ctgctcgatc attacaagat caatctgagt gggctacacg
     4681 ctgtcatcgt tggaagatca ccgattctag gaaaaccaat ggcacaactt ctactgaatc
     4741 gggattgcac catcactgtg tgtcattccc gcacaaaaga tcttccgcgt cacctgcaga
     4801 gcgcggacct tgttgtggca gcatgtggaa agccgcgtta catcgtaggt gattggatca
     4861 aagaaggagc catcgtggtg gatgcaggct acaacgacgg aaacattggt gacgctgatt
     4921 tcgaaagctg tgcgaagaaa gctagctgga tcactccggt tccaggcgga gtcggcccca
     4981 tgaccatcac cactctcatc gcgcacacag tagaaagcgc agaaaaagct tccggttaaa
     5041 tcagcggttt acgattctct caagaatgat cgcacaggcc acgcctgcgt tcagtgaacc
     5101 tgttttgccg tgcattggaa tggtcataag ataatctgcg cgctcaatca agatcgattt
     5161 gattcctgtt ccttcacttc ccacaagcag gcacagtttc tccgcttcag gaagatcatc
     5221 cgaatcggtt gtgcgaagga atgtttgtcc tctggccggt tctgtttcat ccggaactga
     5281 ggcgatgatc cagatccctg cttctttcat tctttctaca aattgaggaa gatttccaac
     5341 attataggcc ggaatgtgaa ggcttgcacc gctcgatgct ctgtgaactg ctggagtgac
     5401 aggggaggtt ttgcttcctg aaaaaacagc gcgcactccg agagcttctg cagtgcgcag
     5461 gatgcttccc aggttctgtg gatctgaaac ttcgtcgagc gccaggaaag gtccgcccag
     5521 agcgagtgcg tcttttgctg tcgcaggagc ggctgctctg taagaacctg ccagctcaag
     5581 caccactccc tggtggtgaa tgccaggaaa cattctgtct atttctcttc ttggcaattc
     5641 ctgaactgca ccgcccagtg ccatgacctg cgatctgaga tcgtttggaa aactttctga
     5701 caaatagatt ttattcactc gcggcggatt gtctgcgcgc aaaaactcgc gcacagtttg
     5761 tctgccgtaa acgatgttgt ctctcaagcc ttctcccagg tggttccctg aggtgaatcc
     5821 ttcaagatga ttccctttgc tttgagttcg tctctgatct gatcggaaag tgcccagttt
     5881 ttttccgcgc gagcacggag tctttcttcg atcttgcttt gcacgtaaga tgcaagatct
     5941 cccagatccg cctgtggttt ttcgcgctgg tcttccgggc gaaaatcaag cactgcgatg
     6001 atctggttgg ctgcgtagaa aaacacaact gcctgtctgg ccagatctgc atccatgcct
     6061 cttgaattca ggtcgcttac agcatcaaag acagcggcca gggcgcgtga aatgttcaga
     6121 tcgtcgagaa gtgcatcctg gaaagattga atcgcagtgg cgaggccctt gtccgcgaat
     6181 agtttttgag gctgcgccgg gtcgcgcgct gcgaaagttg tggccgcttg tttttcagtg
     6241 atcccggaag tgccgagagc tttgtccaga caggtctgga ttctttcaca cgctgcatct
     6301 gatgctttga ggttttcgaa agtgaaattc aagcgagagc gatagtgagt agacaagagc
     6361 agataacgta gagcgcgttt catggagagc ttttcatgca gcttcattgt atctgcggga
     6421 gcgcgatttt cagagatgag tttttcgagt ccttgctttt cgctcagatc gcgaagggtg
     6481 aagtagttgc ccagagattt ggacatcttc tttccgtcca caaggagatg ctcgttgtgc
     6541 acccagtagc gtacgaagtt ttctccgcgg taagcgcagc aggattgagc aatttcgttt
     6601 tcgtggtgtg gaaacaataa atcaattcca cctgcatgta tgtctacacc accggctccg
     6661 tatatatctc ggatcatggc agagcattcc aggtgccatc caggtctgcc ctttccatac
     6721 ggtgaatccc aggctggttc agaaggcgct tgttgttttt tccagagtgc gaagtctctt
     6781 atatcttctt ttgtatattc gtctgctgtg aatcttccgc ccgcagctgc tttcagttgt
     6841 tccggttcga tgcgcgaaag cttcccgtac tccgagaaag atttcagtct gaaatagaca
     6901 tctccatcct gagaataggt gtgcccgctt ttttccagtt tctgcatgag atctatcatc
     6961 gcggggatgg aatcggttgc ctttggtcgg tgctctacat cctgaatgcc caggtatgct
     7021 agatcttcca ggaaatattc aatgaaaggc tttgtgaaag cgaagatgtc ttttccctgc
     7081 tttgctgcat tcgcgatgat cttgtcttcc acatctgtga tgttggttgt gtgatccact
     7141 ttgtatccaa agaagagcag ggacctgcga agcacatcca cagacatgta ggatctgaag
     7201 ttgccgatgt ggttgaaatt gtaaaccgtc ggaccgcagt tgtagagttt cactacacca
     7261 ggctggccag gttggaactc ttcttttttt ccgctcagag agttgaatag acggaaagac
     7321 atatggtcat gggcgtcgtg gagcgtcatt cgtcaaggat acagcgtgta ggtagtgcga
     7381 taatgcatgt aattctggaa agtcacgcgg cagtagttac gggattcttt ggaaggaata
     7441 ttctcgagga acagattgaa atcaccgtga tagtactgtg ccttccatct ggccagactt
     7501 ccgggtccgc cattgtaagc gatagacgcc cagcggaagt
//
LOCUS       DAOQJL010000138         7492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5406_length_7492_cov_5.341804, whole genome shotgun sequence.
ACCESSION   DAOQJL010000138 DAOQJL010000000
VERSION     DAOQJL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_7492_cov_5.341804"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..2978)
                     /locus_tag="PLG78_07900"
     CDS             complement(138..2978)
                     /locus_tag="PLG78_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23075.1"
                     /translation="MSTSESITHQAAAEFFHTSLLSRQSASSRIVMQGGQAAKTIETL
                     HSMLCEMLLKDSRLARTRKLENFPKMSTILADKDGAFQITLGRGLPAESFRASFLGWL
                     AFLRSEGVIEILPALESRGGEIVCNDYLVLQEDKEQDLQLDSFKNESLKTSIDAAGAW
                     LSSFAWSHGVADDLMEKKDPTFVRLLPYYQMLEAMRSDWHAPKDTSQAGFADQIKGKA
                     ELLGKIYNALLGSEKLKFQTKLSGVKKLGDRFPFAWPGSSNAMNDLAAFHDRVRLVFA
                     RDVIMSEKVPGTKPDPKSQFHHANVTAEAFQTAYQSYLKSPDVTPSPYLAHKMGAGLL
                     QQHAHLFSQKEDALRAEPGVLFARNAIVFMLMHSEAVVDHQKEKEKIQFYQAVELYVQ
                     KLASANPPYVDLNVFVRLDKNIVKNENLKKRMIDALRSHQNVKSLEIQDNPTFGHSKL
                     IYLAFHTSQLESMCARSYKAARENKDYNLHMNLERIYDCVQDPSRFQPLVRPDTYERF
                     LQIRQDFFVSQMPFFKRLFWKLFSFARKIDPEQIKDFMKTAMRTEVHKAASIKEKELR
                     TAARKEQERMAKRAVDGSGPHETEPGEIQEHVLDLESTSSSGPSTAPRATSGGRPDPF
                     TEARERSQPDPFSRGATQSRSSERPDFKMPESSTRTEAGRAESSPGVDVSRSSSRSDS
                     TMDRQTTVTRESGSPAGVEVRRENPAASGEPRRAETAAAERRPEERKEERRDEKREEG
                     TRSEEVAGGETGRSSSRLSLEELSAKADRPQVDPDHVQFLKDRPKGAAPQGPQQPQVP
                     EWEIQVIKAGHKVGLDLTERRRLERKKEKEQKKRERHALAVQKLEEHKRKMAAKKKAP
                     RPQQPVPAAAAHAPGTPKHLVIIEVPEKFCAPGKPGRIQFQKKFFKSESFRKEMADFY
                     RKESISASSPEDKKYFAYLISAMEQNYNHYLK"
     gene            complement(3058..4977)
                     /locus_tag="PLG78_07905"
     CDS             complement(3058..4977)
                     /locus_tag="PLG78_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE23076.1"
                     /translation="MRSHLTQIALFILILDLITRRKVVQDFHSREWMLYVASWSAFLI
                     FLHLSVFFAKLLLREGRRFMYASFLISVSLWLAIGTSVSTVFYFTNGIAPNLFSAEFA
                     LREPYNAWTLLRDTASFAHLMWIVCGAALVFHLLKRATTPAPPTPGDGKAAPDTAFEK
                     TALRTLRKGILPAALSLLFMIMVHNVQFYEQCYVPITGNIVILLRSAWNRATNTQLWS
                     RGFTGRNPIQLSDTHEHANFHLLIILNESLRKKGMQIYGGQRQNTPYMQSLKDSGQII
                     LFSRAYANATSTYLSLPSILTGINPVQETELTLTYPNLFEYAKAAGQHTYYISSHKLS
                     WGRMSVFIKTPALEKYWAYEESGAALSNDLGINDLLTLAEWKKHIRETIQNGETMDHQ
                     RTAGMLHFNTTHYPYNVEPEFSLYSGTSRDSYDNTIYKTDALVQEVFKQLTESAMIDR
                     TIVVFASDHGEAFGEHECVAHFYCQYLETVQVPLWIYVPKALQNKLDMQQLKKTAALN
                     VSNVDLIPTIIGLYGMDSSSQISSLKKRFLGSDLFKAVKPDRTILITTSNEMMRTDVG
                     ASIIQNNQQYLLKLARPAHEELYSFDIDPEENHSMWETTEPEARQTWHKWMDSQKATF
                     EIRQRFRKATEHSTK"
     gene            5094..5621
                     /locus_tag="PLG78_07910"
     CDS             5094..5621
                     /locus_tag="PLG78_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23077.1"
                     /translation="MKLFFAFAIFALMRCIPENQIRSYGDPPAAADSFVYATFDIDSN
                     AGKCLLKLHPTDNKGPDQLFAASLLGLAILTVPPGEYELNEVVFATNLQKKEQPFTSP
                     LYLQKFAVQPGEGVYLGNFHCSAVTHGTPQYNQLRIEFGFKLSKYQMNETTRRIVELY
                     GPQLNGRVFRNLENP"
     gene            5618..6346
                     /locus_tag="PLG78_07915"
     CDS             5618..6346
                     /locus_tag="PLG78_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23078.1"
                     /translation="MKLFNIAILIIITTNHCMLMIRTESPQAIYRGARAGVPTKVRCE
                     IVARTSQIYEHPLDNPMTPGQIEMMVRRGLDQQGYSCVNNDSGLPVLTVDAAVYKTHP
                     NPLFFGLTVFLLDLVPTYFGRLTVDVAVSLDIANKPHRFQYTGNYSIWTSMLFIPTGN
                     PEDYGKKKVREIIEDDMAAFAMDAAFVTGAKSEDCFDSDLVLKDGRRIYSARVSRSST
                     AVDVTARDGRHWVFAPEEIVKVDR"
     gene            6461..7093
                     /locus_tag="PLG78_07920"
     CDS             6461..7093
                     /locus_tag="PLG78_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE23079.1"
                     /translation="MKIIASLLFLLGTVNCTYEANCSASDMRCNPILSTGLFIRTCYT
                     QGFCKIFATSGTWFGDLGGSTGVSGADRLCNADANRPAGGGFYAALISDGITRIATVT
                     SNIGDGQVGWVMYPNMEYRLANGSTVWTTNSAGLFVFGTVTNQIAAAGTYYTGMDAFW
                     VSSATCLSWTDGTGGNSGRYGTGGASGNTVISLSQSACNSAMRGLYCVQR"
     gene            complement(7139..>7492)
                     /locus_tag="PLG78_07925"
     CDS             complement(7139..>7492)
                     /locus_tag="PLG78_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23080.1"
                     /translation="ILEVDPIAHTVTSFGNTGGGSNYLGAAVAPNGHIYLTPRSSAVV
                     LEFDPITHTQTTFGSGWGAAVTAFDGGILAPNGKIYAFPLTGSGNNQQPLEIDPKANG
                     NYCPAVARSAYFNKF"
BASE COUNT         1687 a         1802 c         1930 g         2073 t
ORIGIN      
        1 tcaaattatc ctgtggcatg agccgcatca tcagggccag gcccatgcga cgttcaatct
       61 atttaaggcc gccaacggtt tgatggatga gcaaaaataa atatgctggc acaatgagtt
      121 gcatttgagc gcgccactca cttcaggtaa tgattgtagt tttgttccat ggcgctaatt
      181 aagtaggcaa agtacttctt atcttcggga gaggaagccg agatggattc tttgcgataa
      241 aaatccgcca tctctttgcg gaaactttcg ctcttaaaaa actttttctg aaattggatt
      301 ctacccggtt tgccgggcgc acaaaacttt tcgggaactt ctatgatcac aaggtgtttc
      361 ggagtaccag gtgcgtgagc tgctgctgcc ggtacaggtt gctgcggcct gggagccttt
      421 ttcttcgcgg ccattttcct tttgtgttct tctagcttct gcacggcgag cgcatgtctt
      481 tcgcgtttct tctgttcttt ttcctttttt ctttcgagtc gccttctttc cgtgagatcc
      541 agaccaacct tgtggccagc tttgatcacc tggatttccc actccggtac ctgaggttgc
      601 tgcgggccct gaggcgcggc ccctttagga cgatctttca ggaattgaac gtgatccgga
      661 tctacctgcg ggcgatctgc ttttgcggat agttcttcca ggctgagcct gctcgacgat
      721 ctccccgttt ctccgccggc gacttcttcg ctcctggttc cttcttctcg cttctcatcc
      781 cggcgttctt cttttctctc ttccggtctt ctctctgctg cggctgtttc cgcccgtctg
      841 ggctctcctg atgcagccgg attttcgcgt ctgacttcca cgcctgccgg agaacctgac
      901 tctcgtgtaa ccgttgtttg gcgatccatt gtagaatcag aacgtgatga ggagcgactt
      961 acatccactc caggactcga ttctgctctg cccgcttctg tccttgtgct tgactcaggc
     1021 attttaaagt ctggcctttc cgatgaccgg ctctgtgtcg cgcctcttga gaaaggatcc
     1081 ggctggctac gctcacgcgc ctccgtgaaa ggatcaggac gtccgccgga tgttgctcta
     1141 ggagcagtag aaggtcctga agatgaggtt gactcaagat ccagaacgtg ttcctggatt
     1201 tctcctggct ccgtttcatg tggtccggag ccatccacgg cgcgtttggc catccgttcc
     1261 tgttctttgc gcgctgcggt acgaagttcc ttttctttga tactggctgc tttgtgaact
     1321 tctgttcgca ttgcagtctt catgaaatcc ttgatctgct ccggatctat ttttcgtgcg
     1381 aagctaaaga gtttccagaa gagccttttg aagaaaggca tctgtgatac aaaaaagtcc
     1441 tgtcttattt gaagaaagcg ctcgtatgtg tccggtcgaa ccagtggctg gaaacgagaa
     1501 ggatcctgga cgcaatcgta gatccgctcc aggttcatgt gtaagttgta atccttgttt
     1561 tctcgggctg ctttatatga gcgagcgcac atgctttcca gctggcttgt gtgaaaggct
     1621 agatagatta gtttagaatg tccgaaagtt gggttatctt gaatttccag gctctttacg
     1681 ttttgatgcg aacgaagagc atcgatcatt ctctttttca ggttttcatt ttttacaatg
     1741 ttcttatcca atcgaacaaa tacattcagg tccacgtaag gtggattggc tgacgccagt
     1801 ttctgcacgt acagctctac cgcctgatag aattggatct tttctttttc tttttgatga
     1861 tctacaactg cttcggagtg catcagcatg aatacaattg cattgcgagc aaatagtacg
     1921 cctggttctg cgcgaagtgc atcttctttc tgcgaaaaga ggtgagcatg ctgctgaagc
     1981 agaccggctc ccatcttgtg cgcaagataa ggagaaggag tgacatccgg cgattttaaa
     2041 tagctctgat aggcagtctg gaatgcctcc gctgtaacat tcgcatgatg aaactgagat
     2101 ttcggatctg gttttgtccc gggcactttc tcagacatga tcacatcgcg tgcgaaaaca
     2161 agacggactc tatcatgaaa ggccgccaga tcgttcattg cattcgatga accgggccag
     2221 gcaaatggaa accgatcccc cagcttcttt acaccggaca atttggtttg aaattttagt
     2281 ttttctgatc caagaagtgc attataaatt ttcccaagca attctgcctt gcctttgatt
     2341 tgatctgcga aacctgcctg gcttgtatct ttgggcgcat gccagtctga tcgcatggct
     2401 tcgagcatct gatagtaagg gaggagccgc acaaacgttg gatccttttt ttccatcagg
     2461 tcatccgcga ctccgtgact ccaggcgaat gaactcaacc atgcgcccgc cgcgtcgata
     2521 gaggttttca atgattcatt tttgaagcta tcaagttgca gatcttgttc cttgtcttcc
     2581 tggagaacta gatagtcgtt gcagacgatt tctccgcctc tgctttcgag agcgggtaga
     2641 atttcaatga caccttctga gcgcaagaat gcaagccacc caagaaagct ggctcgaaaa
     2701 ctttctgcgg gaaggccgcg gcccagcgtg atttgaaacg ctccatcctt gtccgcaagg
     2761 atcgtagaca tctttgggaa gttctccagc tttctagtgc gagcgaggcg tgaatctttg
     2821 agaagcattt cacatagcat gctgtgcaga gtctcgatgg ttttggccgc ctgcccacct
     2881 tgcattacaa tgcgactact ggccgactgt ctggatagaa ggctggtgtg gaaaaattcc
     2941 gctgctgcct ggtgtgtgat gctttctgat gtagacatga acggcccgga aatgacatga
     3001 aaagaagtag aatctttcgt caaggattgt tgagatttga tcctgttttc agtgtgatta
     3061 ctttgtagaa tgttcggttg cttttctaaa acgctgcctg atttcaaagg ttgctttttg
     3121 agaatccatc catttgtgcc aggtttgtct tgcttctggt tccgtggttt cccacatgga
     3181 atggttttct tccgggtcta tgtcgaatga atagagttct tcatgtgcag ggcgggccag
     3241 tttaagcaaa tattgttgat tgttctggat gatgcttgct cccacatccg tacgcatcat
     3301 ttcattggag gtcgtaatca ggatcgttcg atcgggctta actgccttga agagatcgct
     3361 gccaaggaat cttttcttga gggaggagat ttgcgagctt gagtccattc cgtaaaggcc
     3421 gattatcgtt ggaatgagat ccacgttcga aacgttcaat gcggctgttt tcttcagttg
     3481 ctgcatatcc agtttgtttt gaagtgcttt cggaacatag atccagagcg ggacctgcac
     3541 cgtttccaga tactggcaat agaaatgagc cacgcattca tgttcgccaa acgcctcacc
     3601 gtgatcggat gcaaatacta caatcgtgcg gtcgatcatt gcagattccg ttagttgctt
     3661 aaagacttct tggaccagtg cgtctgtctt ataaatcgta ttatcatagg aatcacgact
     3721 cgtgccagaa tagaggctga attccggctc cacattgtac gggtagtgag tggtattaaa
     3781 atgaagcatc cccgcggtcc tttgatgatc catggtttct ccgttctgaa tcgtttcccg
     3841 gatgtgtttt ttccattctg cgagagtcaa tagatcgttg attccaaggt cgttggaaag
     3901 agcggcacca ctttcttcat aggcccagta tttctccagt gcgggcgtct ttatgaatac
     3961 ggacattcga ccccagctga gcttgtgcga tgatatgtag tacgtatgtt gtcccgccgc
     4021 cttcgcatat tcgaagagat tgggataggt gagggtgagc tctgtttctt gcaccggatt
     4081 aattccggtg aggatgctcg gaagagacag atacgtggat gtggcgtttg catacgctcg
     4141 cgaaaacaga atgatctgac cgctatcctt cagcgattgc atatacggtg tgttctgcct
     4201 ttgtccgcca taaatttgca tgcctttctt acgaaggctt tcgttcagaa tgatgagaag
     4261 atgaaagttt gcgtgttcgt gtgtatcgct cagttgaatt ggatttcttc cagtgaagcc
     4321 acggctccag agttgtgtgt tcgtggctcg attccacgcg gatcggagta ggattacgat
     4381 gtttcccgtt atgggaacat agcactgttc gtagaattga acgttatgca ccatgatcat
     4441 gaagagcaaa gatagtgccg ccgggaggat gccttttcgc aatgttcgaa gcgcggtctt
     4501 ttcaaaggca gtatccgggg ctgcctttcc gtcaccgggt gttggagggg caggtgttgt
     4561 ggcgcgtttg agcaagtgga acaccagggc cgcgccgcaa acgatccaca tcaggtgggc
     4621 gaacgaggct gtatcgcgaa gcaaagtcca ggcattgtaa ggttctcgaa gtgcgaactc
     4681 cgccgaaaag agattgggcg cgattccgtt cgtgaagtag aagaccgtgg aaacagacgt
     4741 tccaatggcc aaccataggg agacgctaat aagaaaagag gcgtacataa atctccgtcc
     4801 ttcgcgtaac agtagtttgg cgaaaaaaac ggacaggtgt aagaagatga gaaacgcaga
     4861 ccatgatgcg acgtacagca tccattcacg actgtgaaaa tcctggacta ctttcctgcg
     4921 cgtaatgaga tcgaggatca atatgaaaag cgcaatctgc gtcagatgag accgcatgat
     4981 tcttctaaat aatgtcacga tggcactact tgtgaaatgc gttcgccggt cacccaattc
     5041 gcgtgtgagc gaaaatgatt gacagaagtt ttttctaatc cccagttgcg cgcatgaagc
     5101 tgttctttgc tttcgccatt ttcgctctca tgcgatgcat tcccgagaac caaattagaa
     5161 gctacgggga tccgccggcg gcggcagata gtttcgtata tgcgacgttt gatatagact
     5221 ccaatgccgg aaaatgtctg ctaaagctcc acccgacaga caacaagggg ccggatcaac
     5281 tgttcgcagc gagtctactc ggcctggcaa ttcttactgt gccccccggc gagtatgaac
     5341 tcaatgaagt ggtttttgca actaatttgc aaaagaaaga gcagccgttt acgtcgccac
     5401 tctatcttca gaaattcgca gttcaaccag gggagggcgt ctacctgggt aattttcatt
     5461 gttcagctgt tacccacggc acgccacaat acaaccagct ccggattgag tttgggttca
     5521 aactgagcaa gtaccagatg aatgagacaa ctcgaagaat tgttgaactc tacggtccgc
     5581 aactcaacgg ccgcgttttt agaaatctgg agaacccatg aagctcttta acattgcgat
     5641 tttgataatc attacaacaa atcactgcat gctcatgatc cgaacagaat cgccgcaggc
     5701 gatctatagg ggtgccaggg ctggagttcc tacaaaagtt cggtgtgaga tcgtggctcg
     5761 cacatcgcag atctatgaac acccgctcga taatccaatg acacccggtc aaattgagat
     5821 gatggttcgc cgtggtttgg accaacaagg ttacagctgt gtcaacaacg actccggttt
     5881 gcctgttctc accgtcgatg ccgcggtcta taaaacgcat ccgaatccgc tatttttcgg
     5941 gctgacggtt ttccttctcg atttagttcc cacctacttt ggcaggctga ctgtcgacgt
     6001 cgccgtttca ctcgatatag caaacaagcc tcatcgcttt caatatacgg gcaactattc
     6061 tatctggact tctatgctct ttatcccgac cggcaatccc gaagactatg gtaagaaaaa
     6121 ggtgcgcgaa atcatcgagg acgacatggc tgcttttgca atggacgctg cgtttgtgac
     6181 gggagcgaaa tcggaagatt gctttgactc ggacctggta ctcaaggacg gcagacgcat
     6241 ttactccgca cgcgtttcac ggtcttcgac agctgttgat gttactgcac gcgacggacg
     6301 gcattgggtt ttcgcgcctg aagaaatcgt gaaggtggat cgatgaatgt tgtggaccga
     6361 ttgatgttcg ggtcgcatga aagtccgcgt gtgctcacaa tgcgctcgac aaaccgtcga
     6421 gtcttcgcca ggatacctcc gggacacgat gatgtttcgg atgaagataa tcgcatcact
     6481 ccttttcctg ctgggaacgg tgaattgtac gtatgaagcg aattgctcgg ctagcgacat
     6541 gcgatgcaat cccatacttt cgactggcct gttcatacga acgtgttaca cgcaaggatt
     6601 ttgtaagatc ttcgctacct ctggcacatg gtttggagac ctgggtgggt ctacaggagt
     6661 gagcggcgcg gatcgactgt gcaacgcgga cgcaaacagg cctgcgggtg ggggtttcta
     6721 tgctgccctt atcagtgacg gcataacgcg cattgccact gtcacttcga acattggcga
     6781 tgggcaggtt ggctgggtga tgtatccgaa catggaatac cgcctggcca atggatctac
     6841 agtctggacc acaaattcag ctggtctgtt tgtgttcggg actgtcacga accagattgc
     6901 tgctgcaggc acctactata ctggaatgga tgcattctgg gtaagcagcg cgacctgcct
     6961 ctcatggaca gacgggaccg gaggaaatag tggcaggtac ggtaccggcg gtgcgtccgg
     7021 aaacactgtg atctccttgt ctcagtccgc ctgcaattca gccatgcgcg gcctgtattg
     7081 cgtccagcgt tgacgttagg cggcattcaa atactttgaa cgcttggtcc ctgcgcagtc
     7141 aaaacttgtt gaagtaagcg gatctggcga cggcaggaca gtagttgcca ttggctttcg
     7201 gatcgatttc aagcggctgc tgattgtttc cgctgccagt aagcggaaac gcataaatct
     7261 ttccattggg agcgagaatt ccaccgtcga acgccgtcac cgcggctccc cacccggatc
     7321 caaacgtggt ttgcgtatgg gtgatcgggt caaattcgag aacaacagca gaacttcgcg
     7381 gggtcaggta gatgtgaccg ttaggcgcca ctgcggcgcc cagataattg gaccctccgc
     7441 ctgtgtttcc aaaactggtg actgtgtgcg ctatcgggtc tacttcgaga at
//
LOCUS       DAOQJL010000139         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5419_length_7476_cov_4.786417, whole genome shotgun sequence.
ACCESSION   DAOQJL010000139 DAOQJL010000000
VERSION     DAOQJL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5419_length_7476_cov_4.786417"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLG78_07930"
     CDS             complement(<1..678)
                     /locus_tag="PLG78_07930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE23081.1"
                     /translation="MMRWKPVLLLFAIFGFLAVAFALFFGALSIFDSPLYAFRLLAYG
                     NSDTDDFKIFAERRIEKASLPSFIERTTLDIPAQVSLPYSGEQRTENIEEFLKKADTR
                     AFLVIKDDRIAVEKYWNSSRDSINTSFSVVKSFDSAMIGAAISDGLIGSVDDPVIEYI
                     PEIAGRGLDQLTIRNLIRMDTGIEYKHNFEIPFFRIPFGDDARTYYGGNLHRVAFSVR
                     PSASPIGP"
     gene            complement(758..2020)
                     /locus_tag="PLG78_07935"
     CDS             complement(758..2020)
                     /locus_tag="PLG78_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE23082.1"
                     /translation="MNYIRLTLHSLKNRALTTSLTVLSIGLSIGLFFGVERVRTAARE
                     SFEGTISGAQLLVGARTGGVQLLLYSIFHIGDSTNNVSYQSFKDWSGRKGVLWTIPFS
                     LGDSHRGYRVVGTNENFYKYYKFRDSRSLEFTSGAPNADLFDVVLGSEVAEKLHYGLG
                     EKVLVSHGVAAKTLESHEDKPFTVRGILKRTGTPIDRSLFISLEGMEAIHIDWKGGYA
                     PSEEERISADRVRKMKIKVDTVTAFLVGLQNRSQVLTFMRNVNDYKEEPLTAVVPAYA
                     LQQLWRIVGYAEDALRIISVFVVLVGITGMILSIYTTLNERRREMSILRSLGARSHHI
                     LLLLVSEAFLLTVAGCITGIVFVYALLGGLQPIIEDQFGIFIPVQMPTLMEFGYLGCV
                     LAAGILAGLIPAWTAYRNALHDGLSVRV"
     gene            complement(2017..2769)
                     /locus_tag="PLG78_07940"
     CDS             complement(2017..2769)
                     /locus_tag="PLG78_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE23083.1"
                     /translation="MNAIEIKDLRFAYSHGPEILEMPEFHVRQGEKVFLFGPSGSGKT
                     TLLSIITGILSARSGRVSVLGEDLTRLSASARDRLRGTRMGYIFQMFNLLPYLSVIDN
                     ILLPCKMNPHRAERVGADVEAEAWIIAEHLGIAEHAHKKPSDLSVGQQQRVAAARALL
                     GNPDLIIADEPTSALDTDHRESFLELLFTNTKKSGAAVLFVSHDRSLARLFDRSISLP
                     DLNLAAPSVSPAIKKPATKVTRTSSGKRVQKK"
     gene            complement(2766..3308)
                     /locus_tag="PLG78_07945"
     CDS             complement(2766..3308)
                     /locus_tag="PLG78_07945"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNE23084.1"
                     /translation="MNRIPLTISIAFLTILVPIFAEPHREHGAHEHGIASLDVTVDGN
                     KVDIDLHSPAVNIIGFEHSARTAADKARQQAGLDRIRKNLSVLFQFDPSRGCKIKIKD
                     LKIETEQHADHPGEKTGEHSELDGEFEVTCAKSPAGSKLKFAFTKIFRDFNLTKIRVQ
                     VMSDHPVSTVIENDEGSVEL"
     gene            complement(3999..5285)
                     /locus_tag="PLG78_07950"
     CDS             complement(3999..5285)
                     /locus_tag="PLG78_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23085.1"
                     /translation="MPQTKILLVALALNGALFAQEVPRDPATENKPTTEPAKQADPAQ
                     TNTQTPSSQTPALPDASIQVSLQLVSNSVFRGDDTFKGRAQQKRKQYGTHSGEWMFQP
                     SITFNTPLEGLTFNWVGSFALAGRQDTDVDQRIESGRDGNETTFFDSYYGSIASGSST
                     AAAFAQARTDLINTFGGVGPVPAATANSFPTRAVADYSIYAPYKEANGLRRLDEMDWT
                     LGYERQTKIGTMGGGLLVQTMSDATGKANPFISGGSGYILTEFFLTYALPQFKDLKLY
                     AGADVIRSDQYYKLTYSHEFPINDSMSVSVNTGPGYMVTQNLQGVRDVPTTVQLNVKG
                     FNVGFNAVYRPKLEFFDTDTATTRAVWLDGGSTKADGLVADPSKYQAGVVSDAINGAI
                     TQAVRTRSGNTNYNYVPRQKLPHWLYFVNLGYTSTF"
     gene            5399..6802
                     /locus_tag="PLG78_07955"
     CDS             5399..6802
                     /locus_tag="PLG78_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23086.1"
                     /translation="MNRKPLFGLKLDLLQGAFARLSKEALAIRYGIRPNRPIELSWKE
                     SPHQPGLRIIEATAGPKVDAGLIVGSVRMGYGHHRMAYSVSSWAIARGETPYLHDLLA
                     IDSPESKLIRESDDLYSFLSRLASEWGGPVEWAWGMVTMNGDLSSLQFSTLLAEGLRN
                     LITDIKPILPLVSSYPLNGQMAVAAGFRRVVHLVPDNHPQYYLLVPGALNLVQSNGAY
                     AKFIQMGVPAENLAVAGHWVSHDIAVNAEADSRVRMERLDAKKPVRVLFSVGGAGAQK
                     SYLSRFIQAIVDLIRSGQVHLWVNAGDHAEVADAVRTAFDKLKIGYSTVESFSDLQTF
                     CSAYPLHGDDPARNAVVFLFSSHYEAFTATDLLIRRCDLLSTKPGELAFFPVPKLFAK
                     RVGDHEAASAVRSMELGEGTAECRSPSDAAALLRKMISDRDLLRQMSEAVIQNAADGI
                     YNGSKTAVDIALSMKDE"
     gene            7143..>7476
                     /locus_tag="PLG78_07960"
     CDS             7143..>7476
                     /locus_tag="PLG78_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23087.1"
                     /translation="MDGDPAYDRAQQQRQQMRDYNNQQRWNSANTELQRSYERAQENA
                     RQESMRRTTNTSPTTTNPGLNAGKSNPASRPEPGQKLDEPAIREQLRRQYLLQTRFET
                     AIKSDDVDE"
BASE COUNT         1824 a         1788 c         1929 g         1935 t
ORIGIN      
        1 cgggccaatc ggactggcag acggccgcac gctgaatgca acgcgatgga gattccctcc
       61 atagtaagtg cgcgcgtcgt ctccaaatgg aatgcgaaag aaggggatct cgaagttatg
      121 tttgtattcg attccagtgt ccattcgaat cagattgcga atggtcaact gatcgagtcc
      181 cctgcctgca atttcgggaa tgtattctat tacagggtcg tccacagacc cgatcagccc
      241 atccgaaata gccgctccaa tcatggctga atcaaatgat tttactacgg agaaagaagt
      301 attgatggag tcccgcgaag agttccagta tttctcaact gcgatgcgat catccttgat
      361 cacaaggaat gcacgtgtgt ctgctttttt gagaaactct tctatgtttt ctgttctttg
      421 ttctccacta tagggcaggg atacttgcgc agggatgtcg agagttgttc gctcaatgaa
      481 agaaggcagt gaggcctttt caattctcct ctccgcgaat atcttgaagt cgtccgtgtc
      541 agaatttcca taggccagga gtctgaatgc atagagtgga gagtcgaaaa tggaaagggc
      601 cccgaagaac agagcaaagg ccactgctag aaagccgaag atcgcgaaca gcaagaggac
      661 cggcttccat cgcatcatga gccgagtgtg atggtcctct tcagtgtgcg caaccgtttt
      721 tagatgcggc ccgcgcggtc aaaaagatgc aagatcttta gactcgaacg ctcaaaccgt
      781 catgtagagc attcctgtat gcagtccagg cggggatgag gccagccaga atgcctgcag
      841 ccaggacgca tcccagatag ccaaactcca taagcgttgg catttgcacg ggtatgaaga
      901 tcccgaactg atcttcaatg atcggttgca gtccacctaa cagcgcgtaa acgaaaacaa
      961 ttcccgtgat gcaaccggcg actgtaagta ggaacgcttc tgatacgagc agaagaagaa
     1021 tatgatgcga tcttgccccg agagaacgaa gaatggacat ttctcttctc cgttcgttta
     1081 aagtggtata tatagaaaga atcatgccgg taattccaac gagtaccaca aagacgctga
     1141 tgattcgcag tgcatcttct gcgtatccca cgattcgcca gagttgttgg agagcgtagg
     1201 ccggaaccac tgctgtgagt ggctcctctt tgtagtcgtt cacgtttcgc ataaaagtga
     1261 gaacctgact tctgttttgc agaccaacca gaaacgcagt cacggtatcc actttgattt
     1321 tcattttgcg cacccggtct gcgctgatgc gttcctcttc tgatggagcg tatccgccct
     1381 tccagtcgat gtgaatcgct tccattccct ccaggctgat gaacaaagat cgatcaatcg
     1441 gagtgcccgt gcgcttaaga attcctcgca ctgtaaaggg tttgtcttcg tgcgattcca
     1501 gggttttggc cgcaactccg tgagaaacga gtaccttctc tccgagtccg tagtgtaact
     1561 tttcagccac ttcagatcca aggactacgt cgaataaatc cgcgttcgga gcgccggaag
     1621 taaactccag tgatctgcta tcacgaaatt tgtagtattt atagaagttt tcgtttgtgc
     1681 cgactacgcg atagccgcga tggctatctc cgagcgaaaa tgggatggtc cacagtacac
     1741 cttttctccc cgaccagtct ttgaatgact gataactgac attgttagtg gagtctccaa
     1801 tatgaaagat ggaatagagc aggagctgga ctccgcctgt tcgtgcgcct accaggagtt
     1861 gtgcgccgct gattgtgcct tcaaaacttt ctctggccgc agttcttact cgctctactc
     1921 caaagaacaa tccaatggat agtccaatcg aaagcacagt aagagaagtt gtaagcgctc
     1981 tattcttgag tgaatgaagt gtcaggcgaa tgtagttcat ttcttttgta cccgtttgcc
     2041 ggatgaggtt ctggttactt tcgtggctgg ttttttgatg gccgggctta cagaaggtgc
     2101 cgccagattt agatcaggca atgaaatcga acggtcaaac aatctggcaa gggatcgatc
     2161 gtggctcaca aacagaaccg ccgctccgct cttctttgta ttcgtgaata aaagctcaag
     2221 gaagctttcg cgatggtccg tgtcgagcgc ggaagtaggt tcatctgcga tgatgagatc
     2281 tggattcccg agaagtgctc ttgctgcggc cactctttgc tgctgaccca cgctgagatc
     2341 ggatggtttc ttgtgcgcgt gctctgcgat tcccaggtgc tctgcgatga tccatgcttc
     2401 cgcctccacg tccgcgccca ctctctctgc gcgatgaggg ttcattttgc atggcaatag
     2461 aatgttatct atgacagaaa ggtaaggaag tagattgaac atttgaaaaa tatatcccat
     2521 tcgagtgccg cgcagtcggt ccctggcaga cgcgctgagc cgggtgagat cttctcccag
     2581 gacggaaacg cgaccggaac gggcggatag aattccagtg atgatggaca acagagtggt
     2641 ctttccgctg ccgcttgggc caaagagaaa taccttttct ccctggcgca cgtgaaactc
     2701 tggcatttct agaatttcag gaccgtggct gtatgcgaag cgaaggtcct ttatttcaat
     2761 tgcattcaaa gctctacact tccctcgtcg ttttctatga cagtggacac aggatgatcc
     2821 gacatcactt gtacacgaat ttttgtcaga ttaaaatccc ggaatatctt cgtgaaggcg
     2881 aatttcaatt ttgaacccgc gggtgatttc gcgcaagtca cctcgaactc cccatccagt
     2941 tcactatgtt cccctgtttt ttcccccgga tgatccgcat gctgttctgt ttcaatcttc
     3001 aagtctttga ttttgatttt gcagccgcgc gaagggtcaa actggaaaag aaccgacaga
     3061 ttcttgcgaa tccggtccag gccggcctgc tgtctggctt tgtctgctgc agtgcgtgcg
     3121 ctgtgttcaa atccaattat attcactgcg ggagaatgca ggtcaatatc cactttgttc
     3181 ccatccacgg tgacatccag agaggcgatc ccatgctcat gggccccgtg ctcacggtga
     3241 ggttctgcaa atatgggcac caaaatggtg agaaatgcaa tagagatggt caggggaata
     3301 cgattcattg tcattgattt tactccagag tcgtatattt gtaaataagt attttcttgt
     3361 ctagaaatat tcctgggtgc tatccgggga aacaacacat ctgcccacgt actttgttta
     3421 atacgagcgc ccggtgtcta tgcggacaaa aaaaggattt atacttgacg atcgttcggt
     3481 tagggagtcc atctaagccg agtcttctat gtatacgctg gaacaatgtt ttcaatctct
     3541 caggaaaaat ggccagaagc tgacccccca gcggatggaa attctgagag tcttgtctga
     3601 ggcaagggcg cccctgactg cacgcgacgt acacactcga gtgaaaaaaa aattctcgca
     3661 tgtaaccctg gatactgtgt accgcaacct tcttatgctt acaggcaccg gatttatcag
     3721 tcatatcaat ttacagaaca agggtacggc gcgctttgaa tttcaggcgg gacatcacca
     3781 ccatcatgcc atctgtctag agtgtggagc ggcgttttgt attgatgttt gttttgtgcc
     3841 agatcgaatc ccggctccca aaaaagacaa aggattcaaa gtcacttcgc acgcatttga
     3901 ggtttatggt acgtgctcaa aatgcgcggc atgacgcttg ctgcgaagca atgcgatccg
     3961 gttttatccg gatcgtctaa aatgttcaga ttttggttct agaaagtgga agtatacccc
     4021 aggtttacaa agtacaacca gtgtggaagc ttctggcgcg gcacgtagtt gtagtttgta
     4081 tttcctgatc gtgtacgtac cgcctgcgtg atggctccgt tgattgcatc ggatacaaca
     4141 cctgcctggt atttggatgg atctgccaca aggccgtctg ctttggtcga tccaccatca
     4201 agccacactg cacgagtggt tgcggtgtcc gtgtcaaaaa attcaagctt tggtctgtag
     4261 actgcgttga atccaacgtt gaatcctttg acgttcagtt gaacagtcgt aggtacgtcg
     4321 cgaaccccct gcagattctg cgtgaccatg tagccaggac cagtgtttac agaaacggac
     4381 atggagtcat tgatcggaaa ttcgtgcgaa tacgttagct tgtagtactg atctgatctg
     4441 atcacatcgg caccagcata tagtttcaaa tctttgaatt gaggtagggc gtacgtgagg
     4501 aagaattcag tgagtatgta tccactgcca ccactgatga atggatttgc tttccccgtg
     4561 gcgtctgaca tggtctgcac gagtaggcct ccgcccatgg tcccaatttt cgtctggcgc
     4621 tcatatccaa gagtccagtc catttcatcc aatctgcgaa gaccgtttgc ttctttgtaa
     4681 ggcgcataga tcgaataatc tgccaccgca cgagttggaa aagagttcgc agtcgccgca
     4741 ggcacaggtc ccactccgcc gaatgtattg atcagatctg ttcttgcctg tgcgaaagct
     4801 gcggcagtgg aagagccact ggcgatggag ccatagtaag aatcaaaaaa agtagtttca
     4861 tttccgtctc tgccggattc gattctctgg tcaacgtccg tgtcctgtcg tccagccaga
     4921 gcaaagcttc ctacccagtt gaatgtgagc ccttcgagag gggtgttgaa agtaatggaa
     4981 ggttggaaca tccattcgcc tgaatgagtg ccgtactgct tgcgcttttg ttgcgcacgg
     5041 cctttgaatg tgtcgtctcc gcgaaagacg ctgtttgaaa ccagttgcag actgacctga
     5101 atgctggcat ccggcagagc gggtgtctgc gaagaaggcg tttgtgtatt tgtctgcgcc
     5161 ggatctgcct gttttgcagg ctctgtggtt ggtttgtttt ctgttgccgg atctctgggc
     5221 acttcctggg cgaaaagtgc accgttcagg gcaagcgcga ccagaaggat tttcgtttga
     5281 ggcatcttca taaagttggc ctgatttatc ggttgcaacc gtaaaagcaa cttgtaaatc
     5341 caacagagtc gaatctggag aaatagttga gactgtatct caatatgggc aatcactgat
     5401 gaatcgtaaa ccattgttcg ggctgaaact cgatttgctt cagggtgctt tcgcacgact
     5461 gagcaaggag gcactggcga ttcgatatgg aatcagaccg aatcgcccca tcgagctttc
     5521 atggaaggaa tcgcctcacc agccaggtct tcgcatcata gaagcgactg caggtccgaa
     5581 ggtggatgca ggtctgatcg taggaagcgt tcgcatgggc tacggccatc atcgcatggc
     5641 ttattccgtt tccagctggg ccatcgcacg cggagaaact ccctaccttc acgatctcct
     5701 ggcaatcgat tcgcctgaaa gtaaattgat tcgcgaatca gacgatcttt atagttttct
     5761 aagcaggctc gcatctgaat ggggcggccc cgtggagtgg gcatggggaa tggtcactat
     5821 gaacggtgat ctgtcctcgc ttcagttctc cacattactt gcggagggtc ttcgcaattt
     5881 gattacagat attaaaccaa tccttccgct tgtgtcttct tatccattga atggtcagat
     5941 ggccgtggca gccgggtttc gcagagtggt ccaccttgta cctgacaatc atcctcagta
     6001 ctacttactg gttccgggag ctttgaatct tgtacagagt aacggtgcgt atgcgaaatt
     6061 cattcagatg ggagtccccg cggaaaacct ggcagtggcg ggacactggg tttcgcatga
     6121 catcgcagtg aatgcagagg cagactcaag agtgcgcatg gaaagactgg acgcgaagaa
     6181 gccagtgcgc gtgctttttt ctgttggcgg cgcgggggcg caaaagagtt atctttcgcg
     6241 attcatccag gcgattgtgg atctgatccg atccgggcag gttcatctct gggtgaatgc
     6301 aggagatcat gctgaagtgg cagatgcagt tcgcactgct tttgataaat tgaagattgg
     6361 ttattcgacc gtggagtcct tttcggattt gcagacattc tgtagcgcgt accctttgca
     6421 tggagacgat ccggcgcgaa atgcagtggt gtttcttttt tcttcgcact atgaggcatt
     6481 cactgcaacg gatctcttga tccgtcgttg tgatcttctc tctacgaaac caggggaact
     6541 tgcgtttttc cccgttccca agttattcgc aaagagagtg ggggatcacg aagccgcttc
     6601 tgcagttcgc tcgatggagc tgggagaagg aacggcagaa tgcaggagtc cgtctgatgc
     6661 agccgctctt cttcgtaaaa tgatttcaga tcgtgatttg ttgcgtcaga tgtcagaggc
     6721 agtgattcaa aatgcggcgg acggcattta caatggcagc aaaacagcag tggatattgc
     6781 gctttcgatg aaagacgaat gatgtgcatt ctgaaaccat aacaggatct tggaaaggga
     6841 gcgaaaagaa cctggttttc aagcaatgac ccgcgtctga atactctgaa acctggttgg
     6901 cgccacactg cgcttcaatg aaaagacatg ccatgctagg acatacgcag cgctggggcc
     6961 tgtcaaacac acgactgccc tgagcatact ccatccgtgc atttattgcg agaggagcca
     7021 tcggatttaa ggcaaatctt ctttacactt gcagagcaga atagtaagct cacgccatgc
     7081 ttctagataa gaggaattct ctcatctttc tgatcatttt gttcgtttct ttgtgcactc
     7141 gtgtggacgg cgatccggca tacgatcgtg cgcaacaaca acgccagcag atgcgggatt
     7201 ataacaatca gcaacggtgg aatagcgcaa atactgaact acagcgctcg tacgaaaggg
     7261 cccaggaaaa tgccagacag gagtccatgc ggcggaccac gaacacttca cccacaacaa
     7321 caaatccagg gctcaacgca gggaagagta acccggcgag ccgccctgaa cccggacaaa
     7381 aactcgatga acctgcaata cgggagcagt tgaggcggca atatttgctt caaacacggt
     7441 tcgagactgc gattaagagc gatgatgttg atgagg
//
LOCUS       DAOQJL010000140         7448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5456_length_7448_cov_4.363452, whole genome shotgun sequence.
ACCESSION   DAOQJL010000140 DAOQJL010000000
VERSION     DAOQJL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_7448_cov_4.363452"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLG78_07965"
     CDS             <1..733
                     /locus_tag="PLG78_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HNE23088.1"
                     /translation="FLVLHTAQKNQDKVEYGETHVFIGYGYIVTVRHGASLPYTTVRE
                     RCEKTPEHLCRGPGFPLYAVMDFVVDNYLPIVEEFEDELVALEETIFKGNSHEDTTER
                     IYDLKREIMGLRRATVPLLEVCNNLTRVTTPLLTAEVQPYFRDVHDHVVRINEAMETL
                     REMLNTAFQVNLSLVTMKQNDVVKKLAGWAGLLGVPTMMASFYGMNFKYMPELEWKYG
                     YPALMVVMISACLFLYYRLKKSDWL"
     gene            768..1832
                     /locus_tag="PLG78_07970"
     CDS             768..1832
                     /locus_tag="PLG78_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23089.1"
                     /translation="MAWYWASLPLVAVLANIAAFLSSLQDRDSFSRGFRVLLVLLAGG
                     NLSLAAVLMSEPSANAPLAFHVFRHFAFLAAPTLASLVRILSGRTLDGWMTRILAVFS
                     FLLIVAIEIDFFSERSLLIDHWASHEWGTFPVLAIRARILVGSLFLLCAGSSIYWLVN
                     AKESLVRKPVTFPITLWWLAFVGNFPALLGVGLYPVGMIFETIVSVLIAATLRKNSAN
                     HWSQKASEVMACSSLSLTIGVLLSALMPENHFKAIAVSLAAGIAAFLGTDRLRSKQIE
                     KKIPGLASYQLTRQEERICELVAEGYTRAQIGFFLGIADGTLRNHLVHLYAKTVDREK
                     ESGVRDKFQRLTLFLLNLQK"
     gene            1922..2641
                     /locus_tag="PLG78_07975"
     CDS             1922..2641
                     /locus_tag="PLG78_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23090.1"
                     /translation="MQTPTYINVYMILASSAVAVAAFGYLRARIESTKERRWWQKSLF
                     VFVALYSIHSVLALSGFYSVFSLPPRFPVAAMATLVIFILFGLSETGRSVLHRIGPDR
                     LALFQVWRFLPEILILLLVRESLLPEAMTAAGRNFDLWIPVTASAMYYLRSRRLLPDA
                     VLIVWNLLGILVLGFTVYTGLRSAPFPFNQPGPIDTSVLAQFPIAFLPLFMVPLAMFS
                     HIAGITLLISNPGADRVPART"
     gene            complement(2604..3836)
                     /locus_tag="PLG78_07980"
     CDS             complement(2604..3836)
                     /locus_tag="PLG78_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23091.1"
                     /translation="MRIDPAFIGFESLEELNRFISDQQLIGAFQFSEPVKDKAGNILI
                     KEKIFVKESAIKRLEAMAGNYDPDFRIAVDPEIIKKLRASLARIIVATLDETESHFIR
                     HLFEKTRSNYNAYIQHAFATDLLVLTFYKQWSDHAEFFRHAAKTGLLALGIIVQKSFG
                     IPRLHRYAFLAGFIAEMPMAASDEWRAQLSDRRRREIAEAASRIAVKLQLPPEVRDAL
                     KNSSLDVEKPANEDVPFDIIDPAHQSGSTEATDDGDDPAPGAPADPASASALTSVVRL
                     ALFIHESSKQIADPAARAEQIVPMVAYNGARGFFHKDLVELIIERFREYEAIARRMMK
                     VAAVEKSCLYPDSAWAYPKPRAAQVLCRHFKTGCPKLESGWDIHVVSPTEAYGWLGTP
                     LAAGNYPKCALERDLPPD"
     gene            3879..4976
                     /locus_tag="PLG78_07985"
     CDS             3879..4976
                     /locus_tag="PLG78_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNE23092.1"
                     /translation="MYDFRLLFVTWVHYDGIMRNILAAFGFLIRLSLKQRKSYSSEYI
                     TINDESIGVDIYDPIGPSKGCILTINGFAPLGNRDERFLHLNSALAGIGFTVVSPHLP
                     GFCNLQIDSAHVSLIQRLTLAISHRRDLTRSGKVAVLAPSFAGGMTLIAAATASSEHI
                     SAICTIGTYANVDALLDSLMLNQNADEYGRLIILRNFLHLSAGRQPRIATAITHILHD
                     SYFHARDPVFPDYEKTLRRTERTFLLRLREDSNFRMRHWKRIIKKSGKRRSILAQICA
                     MKQIKKMDVPVCLIHGCDDNVVPASESRQLHEKFQLLGATSRLILTPLISHGDSRRHL
                     SDLPAAWQLAAGFAFFFKNASTDSRLPDNAR"
     gene            complement(4980..5402)
                     /locus_tag="PLG78_07990"
     CDS             complement(4980..5402)
                     /locus_tag="PLG78_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csp1 family four helix bundle copper storage
                     protein"
                     /protein_id="HNE23093.1"
                     /translation="MTRKEMLLASGAAIAAYKTAGLLADPKDNRGTGIPTLDLSLAAS
                     VCVANGERCVDHCLDLLRNGDDSMGPCSKAAHDMVAICRAMVTLAASKSPMLKNYIVI
                     CKEACETCAAECEKHKMHDPCKRCAESCRACIAAMKAA"
     gene            complement(5399..6022)
                     /locus_tag="PLG78_07995"
     CDS             complement(5399..6022)
                     /locus_tag="PLG78_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE23094.1"
                     /translation="MSCREPDRATEDEHTIMLRIEKTFVRLICILVGMMAVHCRTPRF
                     EVLEPPVSLEGISLVDVDHRVVDTKDILFVVTGYTRCPDICPTTLRSAVMVMDRLPAA
                     SRARFAFLSVDAKNDSPQKTRDYASGFSKRLIALDPSIESSRRFLTLASVFASVDSET
                     ALIQHSTSILWVQDGKIMRKIPVRFASQDLVEEIQDAEREKAERRNM"
     gene            6109..6432
                     /locus_tag="PLG78_08000"
     CDS             6109..6432
                     /locus_tag="PLG78_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23095.1"
                     /translation="MRKITIMFTCVALIIFADCATVQNFGEPRRDPAYVFAGTRANLN
                     DLGNTSDRVDYCFAKLDFPFSFVLDIILLPVAIPLALTWHEKPDPDEDTSPNQAPAKD
                     EADKK"
     gene            6569..6892
                     /locus_tag="PLG78_08005"
     CDS             6569..6892
                     /locus_tag="PLG78_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23096.1"
                     /translation="MGRNRITSVFLSAALLASVAVPSLHALTFDSGKESSREILCGGH
                     SLQIPARILKGTSETFARPGHLILATPSPTLTGHNLVSYTVPGSEIFLKALAHAYHFA
                     RGPPV"
     gene            6932..>7448
                     /locus_tag="PLG78_08010"
     CDS             6932..>7448
                     /locus_tag="PLG78_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23097.1"
                     /translation="MYYLFIKRAMLAPAFLYLASCGGILSQASEDTSTNALIALAALA
                     GSSCASYSEPVISNSLFSGGTRTLYNVSGCDTAGWSRIGFTSDGTTQVSDASKVVTDS
                     SYSGFGTNIEVTFTVTSASGTLDVMTYGTGTFPGTSTTNPTVRIVAGSSCSAQARHSG
                     TGALNNMSTCTD"
BASE COUNT         1770 a         2025 c         1777 g         1876 t
ORIGIN      
        1 gtttcttgtt ttacacactg ctcaaaaaaa ccaggacaaa gtagaatacg gggaaactca
       61 cgtattcatt ggctatggct acattgtcac cgtccggcat ggcgcatctc tcccttacac
      121 aacagtgcgc gaaagatgcg aaaagacccc ggagcatcta tgtcggggac cgggcttccc
      181 tctgtacgct gtgatggact tcgtcgtgga caactacttg cccatcgtgg aagaatttga
      241 agacgaactg gttgcgcttg aagagaccat ttttaaaggc aattcgcacg aagatactac
      301 ggaacgaatc tatgacctga agcgggaaat catgggcctg cgaagagcca ctgttccgct
      361 gctggaagtt tgcaataacc tgacgcgtgt aaccacacca cttctgactg cagaagtcca
      421 accctacttc agagacgttc acgatcacgt ggtgcgaatc aacgaagcca tggaaactct
      481 gcgagaaatg ctcaacactg cattccaggt gaacctgtcc cttgtgacca tgaagcaaaa
      541 tgatgtggtc aaaaagctgg cgggatgggc cggtttgctg ggcgtgccga ccatgatggc
      601 tagcttctac ggaatgaatt tcaaatacat gccggaactc gaatggaagt atggctaccc
      661 ggcgctcatg gtggtcatga tcagtgcatg tcttttcctt tattatcgcc tgaagaaatc
      721 agactggtta tgattcgcgg tcctcactgc catgaggaga acgtctagtg gcctggtact
      781 gggcttcgct gcctcttgta gccgtactcg caaacatcgc ggcgtttctt tcttctctgc
      841 aagaccgcga ttcattcagc cgagggtttc gcgtactcct ggttttgctt gcgggcggga
      901 acttgagtct ggccgctgtt ttgatgagtg aaccttctgc aaatgctcct ctggcgtttc
      961 acgtatttcg gcacttcgca tttctggcag cacccactct agcatccctc gttcgcattc
     1021 tctctggccg cactctggat ggatggatga cgcgaatcct cgcagttttt tctttcttgc
     1081 tgattgtcgc gatagagatt gatttttttt cagaacggtc tcttctgatc gaccactggg
     1141 cctcgcatga atggggcacg tttccggtac tcgccattcg cgcacgaatc cttgttggtt
     1201 cactttttct tctatgtgcg ggcagctcca tctactggct tgtaaatgct aaagaaagtt
     1261 tagtgcgaaa acctgtcacc tttccaatca cattgtggtg gcttgcgttt gtgggaaact
     1321 ttccggcact cctgggagtg ggcctttacc ccgtgggcat gatcttcgaa acaatcgtaa
     1381 gcgtgctgat agcagccaca ctcagaaaga actcagcgaa tcactggagc cagaaggcat
     1441 ctgaggtcat ggcctgcagc tctctttcgc tgaccattgg ggtgctcctt tcggcactca
     1501 tgccagaaaa tcatttcaaa gccattgcag tgtcgctggc ggcggggatc gcagcgtttc
     1561 tgggaacaga ccggctgaga agcaaacaaa tagaaaagaa gatccccggg cttgcatcct
     1621 atcaattgac aagacaggaa gagagaattt gcgagcttgt agcagagggt tacactcgcg
     1681 ctcagattgg ctttttcctt ggaatcgcgg acggtactct cagaaatcac ctggtccatt
     1741 tgtatgcaaa gactgtggat cgcgaaaaag aatccggagt gcgcgacaaa ttccagagac
     1801 tgactctgtt ccttctcaat ctgcaaaaat agcgaaaacc gtgacagatg tcacggttcc
     1861 tggtcacatt cgtgacaggc ggcctatttt caaaatcacg ctcctgtcct agaatgaatg
     1921 catgcaaaca ccaacctaca tcaacgttta tatgatcctc gcaagcagtg cagtggccgt
     1981 ggccgcattc ggctatttgc gcgccagaat agaaagtacc aaagaacgcc gatggtggca
     2041 gaagtctctc tttgtctttg tcgcgctcta ctccattcat tcagtgctag cgttaagcgg
     2101 attctattca gtgttcagcc tgccgccacg ctttcccgtg gcggccatgg ccactctagt
     2161 aatcttcatt ctgtttggcc tgagcgagac tggcaggtct gtccttcata gaatcggccc
     2221 agaccgcctc gccctttttc aggtctggcg ctttctgcct gaaatactca tcctgttgct
     2281 cgtgcgagag tcacttttgc ccgaagcgat gactgcggcg ggaagaaatt tcgatctatg
     2341 gatccccgtc actgcatctg ctatgtatta cttaagatcg cgcagactct tgccagatgc
     2401 agtcctgatc gtctggaatc tgttagggat tcttgtcctg ggattcactg tatatacagg
     2461 cctcagatct gcaccgtttc ctttcaacca gccaggtccg attgacacat ccgttctggc
     2521 tcaatttccg attgcttttc ttccgctctt catggtgccg ctggctatgt tttcgcacat
     2581 agccggaatc actcttctga tttctaatcc gggggcagat cgcgttccag cgcgcactta
     2641 ggataattgc ccgcggcgag cggagtgccc agccatccgt atgcttctgt gggtgacact
     2701 acatggatgt cccatcccga ttccagtttg ggacatccag ttttgaaatg tctgcagagc
     2761 acttgcgcag cgcgcggctt cggatacgcc caggcagaat caggataaag acaactcttt
     2821 tccactgcag ctaccttcat catgcgacgt gcaatggctt catattcgcg gaaccgttcg
     2881 atgataagct ccacaaggtc tttgtggaaa aatcctcgcg caccgttgta ggcaaccatg
     2941 ggaacaattt gctccgccct cgccgctgga tcggcaattt gcttgctcga ttcatgaatg
     3001 aaaagagcga ggcgaacgac acttgtgagt gcagaagcgg acgctggatc tgcgggcgca
     3061 cctggcgcag gatcatcacc atcgtccgtc gcttccgtgc taccggactg atgcgcagga
     3121 tcaataatat cgaatggtac gtcttcgttc gctggctttt ccacatccag ggatgaattc
     3181 ttgagagcgt ctctgacctc gggtggtagc tgtaacttca cagcaatcct ggacgcagct
     3241 tccgcaatct cgcggcgacg gcgatctgac agctgcgccc tccactcatc tgaagccgcc
     3301 attggcattt ccgcaatgaa cccggcaaga aaagcataac gatgaagacg cggaattcca
     3361 aacgactttt gcacaatgat cccgagtgcg agaagtcccg tctttgcggc gtgtctaaag
     3421 aactccgcat gatcactcca ttgtttgtag aatgtaagga ccagcaagtc agtcgcaaac
     3481 gcgtgctgga tgtacgcgtt gtagtttgac ctggtctttt caaacaagtg acgaatgaag
     3541 tgagactccg tttcatccag agtggctact atgatgcggg caagacttgc acgtagcttc
     3601 ttaatgattt cagggtccac tgcaatgcga aaatccgggt catagttgcc cgccatcgct
     3661 tccaggcgct tgatagcact ctcctttaca aagatctttt ccttaataag aatgttgcct
     3721 gctttgtcct tcaccggttc agaaaactga aatgcaccga tcaattgctg atccgaaatg
     3781 aaccgattca attcttccag actctcaaat ccaatgaatg ctggatcaat gcgcatgggt
     3841 gaaaggttcg gtcccgcgcc tttttagcca ccatattttt gtacgatttc cgactcctgt
     3901 ttgttacatg ggtgcactac gatggaatca tgcgaaacat cctggcagct ttcggttttc
     3961 tgatccgact cagcctgaag cagaggaaat cctactcatc agaatatatc acaatcaatg
     4021 acgaaagcat cggagtagat atatacgacc cgatcggacc gagcaaagga tgcatcttga
     4081 ccatcaacgg attcgcccct ctcgggaatc gcgatgagag attcctccat ttaaacagcg
     4141 ccctcgctgg aatcggattc accgtcgtat caccgcacct acccggtttc tgcaatcttc
     4201 aaatcgattc tgctcacgtg agccttatac agcgcctaac gttagcaatc tcgcatcgac
     4261 gcgatctgac gcgatcggga aaagtagctg tgctcgcacc atcctttgct ggaggcatga
     4321 cgctcatcgc ggccgcaact gcttcttccg agcacatcag cgctatatgc accattggca
     4381 cttacgcaaa tgtggatgct cttctggact ctttgatgct caatcaaaat gcagacgaat
     4441 acgggcgact gatcattcta agaaactttc tacacctgtc tgccggaaga cagccgcgta
     4501 ttgcaactgc gatcacacat atccttcacg acagctattt tcacgcaaga gaccctgttt
     4561 tccctgatta cgaaaagact ctgcgacgga cagaaagaac atttctattg cgactgcggg
     4621 aagattcgaa ctttagaatg cgtcactgga aaagaatcat aaagaagagc ggcaaacgtc
     4681 gctcgattct agcgcaaata tgcgccatga agcaaatcaa gaaaatggat gtacctgtct
     4741 gcctgatcca cggctgcgac gacaatgtag ttcctgccag tgagtcgcgg caattgcatg
     4801 aaaaatttca gctcctgggg gcgacttcaa gattgattct cactcctctc atttcacacg
     4861 gggactcgag gcgtcatctt tccgatcttc cggcggcctg gcaattagct gccggattcg
     4921 ctttcttctt caagaatgcg tccaccgata gccgattgcc agacaatgcc cgctaaatcc
     4981 taggctgctt tcatggcagc gatgcatgcg cgacagcttt cagcgcaacg cttacatgga
     5041 tcatgcatct tgtgtttttc gcattcagcc gcacacgttt cgcaggcttc tttgcaaatt
     5101 actatatagt tctttagcat cggcgatttg ctcgccgcca gagtcaccat tgcacggcaa
     5161 atagcgacca tgtcgtgcgc cgccttggaa cagggtccca tgctatcatc gccgttgcga
     5221 agcaaatcca gacaatgatc cacacatctc tctccgttcg caacgcacac cgacgcggcc
     5281 agggaaagat cgagcgttgg aatcccggtt cccctgttgt cttttggatc agcgagaagt
     5341 cccgccgtct tgtaggcagc aatggcagca ccgctcgcca gaagcatttc ctttctagtc
     5401 acatgtttct cctttctgct ttttctcttt cagcgtcctg gatctcctct accaggtcct
     5461 gtgatgcaaa cctgaccggg attttgcgca tgatctttcc atcctgtacc cataagatgg
     5521 aagtgctgtg ctgaatgagt gcagtctctg aatcaacgct tgcaaataca gacgccagag
     5581 taaggaagcg acgcgatgat tcgatggatg gatcgagtgc aattaacctt ttgctaaagc
     5641 cagaagcgta gtcccgggtt ttttgcggcg aatcattctt cgcgtccacg gaaagaaatg
     5701 cgaacctcgc gcgacttgcg gcaggcaatc gatccatcac catgacagca gagcggagtg
     5761 tagtgggaca tatgtccggg cagcgcgtat agccagtcac tacaaacagg atgtcctttg
     5821 tatccacaac gcgatgatcc acatccacga gcgaaatacc ttcgagagaa actggcggtt
     5881 ccagtacttc aaagcgaggc gtcctacaat ggaccgccat catccccacc agaatacata
     5941 taaggcggac aaaagtcttt tctattcgca gcatgatggt gtgctcgtct tctgtcgcac
     6001 gatccggctc ccgacaggac atggttcatc gaccggtgaa gtttgtccat cggttcacga
     6061 ttccctgaat cttgttgcac aatagtctca gcatgaaaga atccgcccgt gcgtaaaatc
     6121 accatcatgt tcacatgcgt cgccctgatt atcttcgcag actgtgcgac tgtgcaaaac
     6181 tttggagaac ctcgcagaga tccggcctat gtattcgcag gaacgcgcgc gaacttgaat
     6241 gatcttggaa acacgtccga tcgtgtggac tattgttttg caaagctcga ctttcccttc
     6301 agtttcgtcc tcgacatcat tctgctaccc gttgccattc cgctggcttt gacctggcat
     6361 gaaaaaccag atcccgatga agacacatcc cccaatcaag cgcctgccaa agatgaggct
     6421 gataaaaagt gaattccact cctgtaattg atctacgtta ggcgatcgca ttaaacagaa
     6481 agcagcccta tgacaaaaat caggccagca agaaacgccc cacgagctct tgcacttgac
     6541 tgtctcctat ccagaagaaa agtgagtcat gggcagaaac agaatcacat ctgttttcct
     6601 ttccgcggca cttctcgcaa gcgtcgccgt gccctcgctg cacgcgctga cctttgattc
     6661 tggcaaagaa tcctcgcgag aaatactgtg tggtggccat tctcttcaga ttccagccag
     6721 aatcctgaag ggcacatccg aaacatttgc tcgccccggg catctcattt tagcaacgcc
     6781 ttcgcccact ctaacgggcc acaatcttgt atcttacacc gtccctggca gtgaaatatt
     6841 cttaaaggcc cttgctcacg cctaccactt cgctcgcggc cctccggtct agatcgttcg
     6901 cataacgatc gttcgattct ggaggaatca tatgtattac ttatttatta agcgagccat
     6961 gctcgcgccg gccttcctgt atcttgccag ctgcggagga atcctttctc aggcatccga
     7021 agatacttct accaacgcac tgatcgcgct cgcagcactc gcaggctcaa gctgtgccag
     7081 ctatagcgaa cccgtgatat caaacagcct gttcagcggc ggcacgcgga ctctttacaa
     7141 tgtaagcgga tgcgacacag cgggatggtc tcgcattggc tttacatccg atggaacgac
     7201 tcaggtttca gacgcaagca aggtcgtaac agattccagc tactcaggct ttggcacgaa
     7261 catcgaagtg actttcacgg tcacctcagc gtccgggact ctcgacgtaa tgacctacgg
     7321 gacaggcaca tttcccggca catccactac aaatccaacg gttcgaatcg tcgcaggttc
     7381 atcttgttct gcgcaagcac gccacagcgg aacgggtgca ctcaacaata tgagcacttg
     7441 cacggata
//
LOCUS       DAOQJL010000141         7402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5506_length_7402_cov_4.063563, whole genome shotgun sequence.
ACCESSION   DAOQJL010000141 DAOQJL010000000
VERSION     DAOQJL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_7402_cov_4.063563"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PLG78_08015"
     CDS             complement(<1..1783)
                     /locus_tag="PLG78_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE23098.1"
                     /translation="MQQFEVFGTRLPGQRDDWLFIGGSALVILIFGALLFWDFNRKNF
                     GGGGEVIGTITFKHRTAQRKFASQVIWDDLDTETPLYNRDAIRTAESSEAVVTLKNGT
                     KIQLDESTMIVLNISDKSADIDFAYGSVQAKQDQKAGQEVAALNIKTQDNKVISIQNS
                     DVKLSRNEGEDLSLTVQRGQANVQTSDGKTQKVEKDQKATIAAEIQVRKVVLRPTAPD
                     DGARSFITENQKAFAFAWEKPTEALTFEIASDRSFSRIITRVTTAGPGTSVALGDGSY
                     FWRLAAQDKTGKIDYSDFRRFTVIRNAPAVLLSPVSGAQFDYVTDDPLVNFTWSRSET
                     AQGYALEIAENPGFASARRIETSTTSISQKLPAGNYFARIVTTSSEAGNLPGAATAFT
                     VVRRQKTQAPLAVRPAGDRIARVILEKEGLAFSWSVDRDVRSTTLEISPNASFDQIAF
                     KGTADTNFLTVKQGFREGKYYWRLQGIDRKGNATDFSNVSSFTVAEMEKVALRGPANG
                     ADMDPVQARLEGVGLLWEGPDLSQFRVVVSPNQNLDPAVVNQMTSARAYSARNLAPGR
                     YYWKVLLVSQNGSVLTESEIRSFSILDL"
     gene            complement(1791..4526)
                     /locus_tag="PLG78_08020"
     CDS             complement(1791..4526)
                     /locus_tag="PLG78_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23099.1"
                     /translation="MIAELKTTDLVLNGGEIKIADGNSWMGAGDGINHALDDLAEIGV
                     QELLIYAQKNEVPKELIRAVKAKGLTASHIQPHNILEGGDFTPVRKHHGALLSRARKH
                     SSAVVYVGHHEPDDLLFFLAGLVLFSHRELTAIEACRTVADSDGNEAVHEALNRYAEF
                     LREESGHSQKNGKSTVVSPSHDDADAHAFLARTAPPAATQPTDVEAVSMAAALSPREK
                     ETEAPVPTPVAEPYSPPEAIEPPAPVPAVVESQKLPEKAETKPAPKPASKPAGGEIRK
                     LDHSFKPSKFTIKVKLLSIISAVIVGALTAMIFLATFFFRRDSERSIQGSNLSITEII
                     GLQLQSEIETISYKSHLMGTTLEQPQAADQKKVFIDLFLQNNAHFMFIGVARRAGDAI
                     IIGQSVTNDKFLKDNNISQEDITRLNQQNGKAFLASFGGATIVHNASQGFKIPLIAVS
                     VPLARGESIVVAYLDPGTFIKAFQQTTQALPGTTAFLVDQAGDVIAHPDSKLVLSGTN
                     LADSPIIKALLESKVDNGQIRYSDKNGWNLGAYKKLSVGGLGIVSTAEENVVFGPVFA
                     IQRRNIYIMVIVVALSFIVVYLFSKTLTVPIVKLVGATQQVERGEYGIDIKPASGDEI
                     GDLTHSFVNMAVGLEEREKLKDAMGKFTNPEIAEMVLRGEMKLGGERKDAAIFFSDSR
                     GFTAMSENMEPEEVVEFLNEYFTDMVNCVDVTGGVVDKFIGDAVMAHWGALFSRGNDT
                     ENSINAAIMMRKALIAFNERPTTKSGKKKPIAMFGCGINTGPVISGQIGSETRLDYTV
                     IGDTVNLASRVESLNKPFGTDILITTDAYDRVKDVFQVEEMPAIKVKGKSEAQIIYAV
                     LGRLDDPERPKSLKELRSLVGIKFDEEKASKMEQAASGEGEVKYEVIGDKSKDAH"
     gene            complement(4599..5429)
                     /locus_tag="PLG78_08025"
     CDS             complement(4599..5429)
                     /locus_tag="PLG78_08025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE23100.1"
                     /translation="MKLRRVSLSVLGFLLASAVFSALPRVAPLMEGLSGSGQSPGYSR
                     VPILCFHDLNGRGRYSITSATFRHFMKRLRDENIAVIPLAEVVERSQNKRLLEKPAVA
                     ITVDDDYEGAVRIAAPILREFGYPATFFVYTGGIHTDPAEGMSYDDLRRLLKEGFDVQ
                     NHSHTHTAFHVPRKGESAAEYEQRLVVEIEDSRKILEKNLMGHKIRFFAYPMGYHTPA
                     LRERVFKAGYELVVTTDARPVDLTRPFNGSFDRRTIELDGGVAELIFEQAIQIAKKAI
                     "
     gene            5533..7056
                     /locus_tag="PLG78_08030"
     CDS             5533..7056
                     /locus_tag="PLG78_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNE23101.1"
                     /translation="MDTTLRDGEQTHGVSFSAFEKLQIARFLLEQVCVDRIEVASARV
                     SRGEHETVKEIFVWAHEKGVADRIELLGFTDHKGSIDWIGEAGGRAINLLTKGSLKHC
                     REQLGKTLAQHLADIEKTVRYGEEHGFRVNVYLEDWSNGFLHSPDYVREMVGALRNLP
                     IHRFLFPDTLGIMSPDEVRAGLRDIFDLYPDIQIDFHGHNDYDLATANCLAAVSEGVV
                     GIHCAVNGLGERAGNSPLEAVVTALHDKLRVQTGVNEKALVDVSRLVETFSGKRISAN
                     HPVVGHDVYTQTAGIHADGDKKNNLYANPILPERFGRQRVYALGKLAGKASVSNNLGM
                     LGIQLPPEKELLLLDRIKQLGEQKQTVTPDDLPFLMEDLFGSGDSRQIRIKSAEIHST
                     LQGKPRAKVTLDFNGQTYEETGEGNGGYDAFMKALELIAKRVGIELPQLMDYEVRIPP
                     GGKTDALVEAMIEWAPAEGRSFRTVGVDCDQVMAAIIATEKMLNRIVQPAADLLRQF"
     gene            complement(7047..>7402)
                     /locus_tag="PLG78_08035"
     CDS             complement(7047..>7402)
                     /locus_tag="PLG78_08035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE23102.1"
                     /translation="NPELVRPLIKYYLEKKNFARTAEIAEPLAEAGHLDNEFAFVTAH
                     CRKKLNRLKEAADMAERVYLREPELAKNLFLLAEVYARMGNIARGADFLDKAEKLDPG
                     NPAVAKVRAMFGTQN"
BASE COUNT         1694 a         1833 c         1895 g         1980 t
ORIGIN      
        1 aaagatccag aatgctaaac gatctgattt cgctctccgt aagaacagaa ccgttctgcg
       61 aaaccaggag cactttccag tagtaccggc ccggtgcgag gtttcgcgca gagtatgcac
      121 gtgcagacgt catctgattt accacggcgg gatccaggtt ctggttcggt gaaacgacca
      181 ctcggaactg cgaaagatcc ggaccttccc acaacaatcc aaccccttcg agacgtgcct
      241 gaactggatc catatcagcc ccgtttgccg gaccgcggag agccactttc tccatttcgg
      301 ccacggtaaa agatgagacg ttggaaaagt cagtcgcatt acctttcctg tcgattccct
      361 ggagtctcca gtagtattta ccttcgcgga atccttgttt tacagtcagg aagttcgtat
      421 ctgccgtgcc tttgaatgcg atttgatcaa aagaagcatt gggcgaaatt tcaagagtag
      481 tgctgcgtac gtcgcggtct acgctccagg agaacgcaag gccttctttt tcaagaatga
      541 cgcgcgcaat tctatctcca gccgggcgaa cagcaagagg agcctgcgtt ttctgtcttc
      601 gcaccactgt aaatgcagtg gctgcacccg gcaaattccc cgcttctgag ctcgttgtga
      661 cgattcgtgc gaaatagtta cctgcaggca gtttttgact gatcgatgtg gtagaggttt
      721 cgattcttct tgcgcttgca aagccagggt tctctgcaat ttcgagcgca tatccttgcg
      781 cagtttcgct tcgcgaccat gtgaagttta cgagagggtc atccgttacg taatcgaatt
      841 gcgcgccaga cacgggcgaa agaagcaccg ccggtgcgtt tcgaatcaca gtgaatcttc
      901 taaagtcgct ataatcgatt ttgcctgtct tgtcctgtgc tgccagtctc cagaagtaag
      961 acccgtctcc cagagcaacg ctggttccgg ggccggccgt tgtgacgcga gtgataattc
     1021 ttgagaagga tctgtccgat gcgatttcaa atgtgagcgc ttcggttggt ttttcccatg
     1081 caaatgcaaa tgccttttga ttttcagtga tgaaggatcg cgcaccgtcg tctggagcag
     1141 tggggcgaag taccaccttt cgcacctgga tttcggccgc aatggttgcc ttttgatcct
     1201 tctctacttt ctgagtttta ccgtctgaag tctgaacatt cgcctgaccg cgctgcacgg
     1261 tgagagagag atcctctcct tcgtttctcg agagtttgac gtctgagttt tgaatagaaa
     1321 tgactttgtt gtcttgcgtc ttgatgttga gagcggccac ttcctggcca gctttctgat
     1381 cctgctttgc ctgcacggag ccgtatgcaa aatcaatgtc tgctgatttg tctgaaatgt
     1441 ttagaacgat catggtggat tcgtcgagct gaatcttggt tccgtttttg agagtgacta
     1501 cagcttccga agattccgca gtgcgaatcg cgtctctgtt atagagggga gtttccgtat
     1561 ccagatcgtc ccagatgacc tgtgaagcga acttacgttg cgctgtgcga tgcttgaatg
     1621 tgatggtgcc gatcacttcg ccaccacctc cgaaattctt tcgattgaag tcccagaaca
     1681 gcagcgcacc aaaaatcaaa atgacaagcg cgctgcctcc gatgaagagc cagtcgtctc
     1741 tttgtccagg aagtcgggtg ccgaacactt cgaattgctg catgatgtcc tcagtgcgcg
     1801 tccttcgatt tgtcgccgat tacttcgtat ttcacttctc cttcgccgga agcagcctgt
     1861 tccattttgg atgctttttc ttcgtcgaat ttgatgccga cgagtgaacg aagttctttc
     1921 agagattttg gtctttctgg atcgtccagt cgcccaagaa ctgcatagat gatttgcgct
     1981 tctgattttc ctttgacttt gatcgcaggc atctcttcta cctggaatac atccttcact
     2041 ctgtcgtaag catccgttgt gatcagtatg tccgttccaa aaggtttgtt caaagattca
     2101 acgcggcttg cgaggtttac cgtatcaccg atcactgtat aatccagtct cgtttccgaa
     2161 ccaatctgtc cggaaatgac cgggccagtg ttgataccac agccaaacat cgcaatcggc
     2221 ttttttttgc cgctcttcgt cgttggtctt tcgttgaatg cgatgagcgc ttttcgcatc
     2281 atgatcgctg cattgattga gttttctgtg tcgtttccgc gcgaaaacag tgcgccccag
     2341 tgagccatca cagcatcacc gatgaactta tccacaaccc cgcccgtaac gtccacgcaa
     2401 ttgaccatat ctgtgaagta ctcgttgagg aattcgacga cttcttccgg ttccatgttc
     2461 tctgacatcg cagtaaatcc gcgcgaatca gagaagaaaa tagccgcgtc tttgcgttcc
     2521 ccgcccagct tcatttctcc gcgaaggacc atttccgcga tttcagggtt tgtgaatttg
     2581 cccatcgcat ctttcagttt ttcgcgttcc tcgagaccca cggccatgtt cacgaatgaa
     2641 tgagtcagat ccccgatttc atctccggag gcaggcttga tgtcgatgcc atattcgcct
     2701 ctttccactt gctgtgttgc acctactagt tttacaatag gaacagtcag cgtttttgaa
     2761 aacaggtaca ctacaatgaa cgacagagca actacgatca ccatgatgta aatgtttcgt
     2821 ctttgaatcg caaatacagg gccaaatact acgttttctt ccgcagtaga tacgatccca
     2881 agtccgccca cgctgagctt tttgtacgcg cccagattcc atccgttctt atctgaatag
     2941 cgaatctggc cattgtccac cttgctttcg agtagagcct taatgatggg agaatctgcg
     3001 aggttagtgc cgctcaatac aagctttgaa tccggatgtg caatcacatc tcccgcctga
     3061 tccacaagga atgcggtggt tccaggaagt gcctgtgtag tttgctggaa cgctttgatg
     3121 aatgttccag gatcgagata cgctacaact atgctttcgc cacgtgcgag aggaacgctc
     3181 actgcaatca gcggaatttt gaatccctgc gaagcgttgt gtacgatggt cgcacccccg
     3241 aaggaggcca ggaatgcttt tccgttttgc tggttgaggc gagtgatgtc ttcctgagag
     3301 atgttgttgt ctttgaggaa tttgtcattg gtcacacttt gaccgatgat gatcgcatct
     3361 cctgcgcgtc ttgccactcc aatgaacatg aaatgcgcat tgttctgtag aaatagatca
     3421 atgaacactt tcttttgatc cgcggcctgt ggctgctcga gagttgtccc catcaggtgg
     3481 gatttgtacg aaatcgtttc aatttcggac tgcagctgca gaccaatgat ttccgtaatg
     3541 ctcaggttac ttccctggat gcttctttcg ctgtctcttc taaagaagaa agtagcgagg
     3601 aagatcatcg cagtgagagc tcccacaatc accgcggaaa tgatggagag aagcttcact
     3661 ttaatagtga atttcgatgg tttaaaagaa tgatccagtt ttcggatttc tccgccggcg
     3721 ggcttagacg cgggtttcgg ggcaggcttt gtctctgctt tttcaggcaa tttctggctt
     3781 tcgactacgg caggcaccgg tgcgggaggc tcaatggctt ctggcggtga gtaaggttct
     3841 gcgactggag ttggaaccgg agcttccgtt tctttttctc tgggcgagag ggctgccgcc
     3901 atggaaacag cttctacatc tgtaggctgc gtggccgccg gaggagctgt gcgcgcgaga
     3961 aatgcatgag catccgcatc gtcgtgtgaa ggagagacga cagtgctttt tccgttcttt
     4021 tgcgagtggc ctgattcttc tctgaggaat tccgcataac ggttcagagc ttcgtgtacc
     4081 gcctcattgc catcagaatc tgcgacggtg cgacaggctt cgattgcggt tagttcgcgg
     4141 tgcgagaata gaaccagtcc ggcaaggaag aagagaagat cgtctggttc atgatgacca
     4201 acgtacacga cagcgctgct gtgttttcgc gcgcgcgaaa gaagcgcgcc gtgatgtttg
     4261 cgcactggag taaaatctcc accttccagg atgttgtgtg gctgaatatg agatgcagtg
     4321 agtccctttg ctttgactgc acgaatgagt tctttgggga cttcgttctt ttgtgcgtag
     4381 atgagcagtt cctggacgcc aatttccgcc aggtcatcga gggcatggtt gattccatcg
     4441 ccggctccca tccaggagtt gccgtctgcg atcttaattt ctcctccgtt taggacgagg
     4501 tctgtcgttt tcaattccgc gatcaaggtt ccgccccctg ccaggtagta cgacgataaa
     4561 aaggtcgtgt caatcgtaat tatcgggcgt ttgtgacgct aaatcgcctt tttcgcgatc
     4621 tgaattgctt gttcgaaaat taattctgca acgccgccgt ccagttcaat ggttcgcctg
     4681 tcaaatgagc cattaaacgg gcgcgtaagg tccactggac gtgcgtctgt cgtcacaacc
     4741 agttcgtaac ctgccttaaa tactctttcg cgcagtgcgg gagtgtggta acccattgga
     4801 taggcgaaaa agcgaatctt atgtcccatt agattctttt ctagaatttt gcgcgaatcc
     4861 tcaatttcca caactaatct ctgttcgtat tccgctgcgg attcaccttt tcgcggaaca
     4921 tgaaaggcag tgtgcgtgtg cgaatgattt tgaacgtcaa atccttcttt caggagacgg
     4981 cgaagatcgt cataggacat gccctctgct ggatctgtat gaatcccgcc tgtataaacg
     5041 aagaacgtgg cagggtaacc gaattcgcga aggatggggg ccgcaattcg aacggctcct
     5101 tcatagtcat catctacagt gatggcaacg gcgggtttct caaggagccg tttattctgg
     5161 gatcgttcca ccacttctgc cagtggaatc acagcgatgt tctcatcgcg tagccttttc
     5221 atgaaatgac gaaatgtggc gctcgtgatc gaataacggc cccggccgtt tagatcgtga
     5281 aagcagagta tgggaaccct gctatagccg gggctctggc cgctaccgga aagaccttcc
     5341 atcagcgggg ccacacgggg cagggctgaa aatacagcag aggcgagcaa aaaaccgagg
     5401 acggaaaggg atactctgcg aagtttcata ggcccagctt tattttcgga gtcgatctgc
     5461 gtttacatag gcggcaatct gaaaattttg gctatcttga taagcaggcc ccctgtgcga
     5521 cgaatagaga tcatggacac aactctccgt gacggagagc agactcacgg agtgagtttt
     5581 tctgcatttg agaagctgca aattgcgcgc ttcttactgg agcaggtatg cgtggatcgc
     5641 attgaggtgg caagtgcccg cgtttcgcgc ggagagcacg agactgtaaa agagatcttc
     5701 gtatgggccc atgagaaggg tgtggcagat cgcattgaac ttcttggttt tacggatcac
     5761 aaaggctcca ttgattggat cggtgaagcg ggcggtcgtg ccatcaatct tcttacgaaa
     5821 ggttctctca aacactgtcg cgaacagctg ggaaagactc tggctcagca tctggctgac
     5881 attgaaaaga cagtgagata cggtgaagag catggctttc gagtgaatgt gtacctcgaa
     5941 gactggtcta acggatttct gcattcgcct gattacgtgc gcgaaatggt cggtgctctt
     6001 cgcaatctcc ccattcatcg ttttcttttc ccggacactc ttggaatcat gagtcccgat
     6061 gaagtacgcg cgggtctcag agatattttt gatctctatc cggacattca aattgatttt
     6121 catggtcaca acgattatga tctcgccact gcaaactgtc tggccgcagt aagcgaaggt
     6181 gttgttggaa ttcactgtgc agtcaatggt ctgggggaaa gagcagggaa ctctcctctc
     6241 gaagcagtcg tcacagcact tcatgataaa ttgcgagtgc aaacaggtgt gaatgaaaaa
     6301 gctctcgtgg atgtttcgcg ccttgtggaa acattcagtg gcaaaaggat ttccgcaaat
     6361 cacccggtag tgggtcacga tgtttacacc cagactgcgg gcattcacgc agacggagac
     6421 aagaagaata atctatacgc caatccaatt cttccggaaa gattcgggcg gcagagagta
     6481 tacgcgttag gaaagctcgc aggcaaagcg tctgtttcca ataacctggg aatgctgggc
     6541 attcaacttc ctccagaaaa ggaacttcta ctcctcgatc gaatcaaaca gctgggtgaa
     6601 caaaagcaaa cagtcactcc ggatgatctc cctttcctta tggaagatct gtttggatca
     6661 ggcgattcaa gacagattcg aatcaagagc gcagaaattc attccactct tcagggaaag
     6721 cctcgtgcga aagtcactct tgacttcaac ggtcaaacgt acgaagaaac aggagaagga
     6781 aacggcggat acgatgcctt catgaaggca ctcgagctca tcgcgaagag agttggaatc
     6841 gagcttccac aacttatgga ctacgaagtg cgcattccac ctggaggaaa aacagacgcg
     6901 ctcgtggaag cgatgattga gtgggcccct gcagaaggca gatcctttcg cacagttggt
     6961 gtggactgcg atcaggtcat ggccgcgatc atcgcaacgg agaaaatgct taaccgcatt
     7021 gtgcaacctg ccgctgattt actccgtcag ttctgagttc caaacatcgc gcgcactttg
     7081 gcaacggccg ggttccccgg atcgagcttc tctgctttgt ccaggaaatc tgcaccgcgc
     7141 gcaatgtttc ccatcctcgc ataaacttcc gcgagaagaa acaaattctt tgcaagctca
     7201 ggttctcgca gatacactct ttccgccatg tccgctgcct ctttgaggcg attgagtttc
     7261 ttgcggcaat gagccgtgac gaatgcgaat tcattgtcca gatgcccggc ctctgcgagc
     7321 ggttctgcga tttctgcagt gcgtgcgaag ttcttctttt ctagatagta tttgatgagg
     7381 ggccgcacaa gttcagggtt ca
//
LOCUS       DAOQJL010000142         7378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5543_length_7378_cov_4.600847, whole genome shotgun sequence.
ACCESSION   DAOQJL010000142 DAOQJL010000000
VERSION     DAOQJL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5543_length_7378_cov_4.600847"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PLG78_08040"
     CDS             complement(<1..1280)
                     /locus_tag="PLG78_08040"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE23103.1"
                     /translation="MAIRVALHHKTSYRYDHAVTAFPHVVRLKPAVHSRTPILSYSFR
                     VGPEPHFINWQQDPFGNHLARLVFPEKTDRLDFTVDLVAELHSINPFDFFLEPDAEQF
                     PFQYSPDVKAQLAPYLALRENGPLLKYYVESNRRKQKTIDFLVELNQRIHSDVSYLIR
                     MEPGVQTCEDTLKLKSGSCRDSAHLLVHVLRGLGLAARFVSGYLIQLKPDETPLSGPP
                     GPAHDFTDLHAWAEVYLPGAGWVGLDATSGLFTSEGHIPLACSPNPEDAAAITGAVEP
                     AKSDFSFEMKVTRIEESPRVSQPYTDLQWKKIQGVGRVVDADLMSHNVRLTSGGEPTF
                     VSQTDRDAPEWNISANGLKKKDLARRLALALRGRFAPGSSFHSGQGKWYPGEEIPRWA
                     YSVFFRKDGRPLWRDDRLLADETRNYNYGPREAR"
     gene            1619..1894
                     /locus_tag="PLG78_08045"
     CDS             1619..1894
                     /locus_tag="PLG78_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNE23104.1"
                     /translation="MELIHALATSLLSPMVRCSVFEVGLVTGRRMQDLKKVGWFLIAF
                     AVVMPLIHGLPGVLIGTWTGLSIGGTCVARISHHLPPDSFYCSHFSF"
     gene            1943..2251
                     /locus_tag="PLG78_08050"
     CDS             1943..2251
                     /locus_tag="PLG78_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23105.1"
                     /translation="MNLHHVKLLTIIAPDNLEARLTSDVRLFGARGYTISPAVGAGLS
                     SVRDNEWTGKNIRLETLVGQDVAYALMAHLAKDYFEKFGVIAFMSDVEVLRKERFDYR
                     "
     gene            complement(2248..2814)
                     /locus_tag="PLG78_08055"
     CDS             complement(2248..2814)
                     /locus_tag="PLG78_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNE23106.1"
                     /translation="MSFSALFSVRSVGIAFLALMISSSAQAQGRAPEHLTDSAQIAMF
                     NRLTSKVNCYCGCHSIIGTCGHIEENCFGVQLRRFIENRIIEGRSESSIEEGLVSGFG
                     EEIRKDPQVIILQAEGRVDLVSGFVKGFGKMIFVKPPVVWPWIVVAIVGAFALLWVWS
                     RRNKPRPVAHARSPEIDSILNRTRELDR"
     gene            2925..5501
                     /locus_tag="PLG78_08060"
     CDS             2925..5501
                     /locus_tag="PLG78_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE23107.1"
                     /translation="MGLTVAIVLGLLLAVCAAWIARELRNRRNQIARQLFLRHGSVML
                     LIKPDDGRIAFANLAAARFYGYSPRQLESMSVKDLNTLGPAEVQAELDRARREERSYF
                     LFRHRVASGDIRTVQVHSSPLVIDGETFLLSIIDDVTHHAKLTKERETRGRVLELLAA
                     NAPLESILTELVLAYEDLAPGSICSILLTDSEESTLLPGAAPGLPEWFNHEVQGLSTG
                     EGQGSCGTAIARKQRVIVSNIATDPLWDKYRHLAERAGLAACWSEPIFSGSRKILGSF
                     AIYYKQVREPHEEDLQLISRHAQLASLCIEQTQLRRRQQLADHVFQNTGEGILITDDK
                     SQILAANPAFGKLTGYEPAEILGKTPRILSSGKHPKSFYEDMWGQIRNTGKWKGEIWN
                     KRKNGELYPELLSISTVYTKGSVIGYYVAIFTDISNIKETEARLDELAYRDPITALAN
                     RSLFQLRLAEALHRAERSGTSVWILLIDLDRFKDVNDALGHEVGNKLLEAVTGRFLEV
                     LEPGDLLARMGGDEFGLLLENADAGRIAQRLLHSLNAPVHLKSGDQIDVGASVGISQF
                     PHDGNSVEQLIQNADAALYDAKASGRRTFRFYTSTLTARAKARLSLETKLRRALERNE
                     ILLHYQPQVNIRTGKIVGVEALARWPDGDQMIPPDQFVPVAEESGLILPLGQSLFRKA
                     CMQARQWNDAGISLMVAVNLSARQCANARLAHEIAEVIRETGVNPEQLELEITETALM
                     SQGQDALNLLEQMRKIGVRMALDDFGTGYSSLAYLRHFPVDTLKIDRSFISEIPTHNS
                     GAEITSAIIRLGHSLNFTVLAEGVETEAQLAFLAKQECDLYQGYYRGRAVPPEEIPAL
                     LQ"
     gene            5584..6633
                     /locus_tag="PLG78_08065"
     CDS             5584..6633
                     /locus_tag="PLG78_08065"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE23108.1"
                     /translation="MEPRLTLPAVRDTISKESLRNPTWKGLLYFARDIAVYAAILVGL
                     YSTDAWYFLVPLWILAGLAVSGLFIIGHDAAHGALFKNQKLAYLIGQISMLPSLHAYH
                     QWVYGHNRIHHGHTIKMEGDFVWRPTSPAQYRDLSWAGKIWHRITWSAIGSGIYYLVQ
                     IWFGGMILFVAQTKGAKRDRILIILAAIAMIAVPIALFPTWQAGLWVAVKLGVIPFLA
                     FNYCIGFSVYVHHIHDFIPWKKHKDWTPFYGQMLGTVNYHIPAFFNFFLHNIFLHMPH
                     HVNMRVPFYNLGRALSEIKSVYGQYVIERKSMFMDYLHTTRKCKLFDPESGKWLTYAE
                     AAEERTPNLSQSVPA"
     gene            6567..7010
                     /locus_tag="PLG78_08070"
     CDS             6567..7010
                     /locus_tag="PLG78_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE23109.1"
                     /translation="MANVRGSGGRKNAKPQPVRAGLSRESILDAALEILNEGGMAALS
                     LRSIVKRLGCSVAGPYTYFAGQDEIISTLIQRGEEELTGDLKRAGQEGGDLFEVLRRL
                     AATYMTFARRNRELHKLMFNINGHKRIFPSQPQAIAPILKPSGVE"
     gene            7064..7195
                     /locus_tag="PLG78_08075"
     CDS             7064..7195
                     /locus_tag="PLG78_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23110.1"
                     /translation="MWAWLYGLIVLEMTGVTTMNADEAIDEGFSFFQRLLEQGEKSA"
     gene            complement(7192..>7378)
                     /locus_tag="PLG78_08080"
     CDS             complement(7192..>7378)
                     /locus_tag="PLG78_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23111.1"
                     /translation="SKYFFKTLEYPEVELGRPMNYFWYADQASHAFYEFGLSKTDLDQ
                     FLSRETKALVVVNPQPR"
BASE COUNT         1918 a         1937 c         1804 g         1719 t
ORIGIN      
        1 attcttgctt ccctcgggcc gtaattgtag tttctggttt catccgcgag caaacgatca
       61 tcgcgccaca gtggccttcc atctttgcgg aaaaaaacag aataagccca gcgcggaatc
      121 tcttctcccg gataccattt gccctggccg gaatgaaaag atgacccggg ggcaaagcga
      181 cctctgaggg caagagcgag acgacgcgcc agatctttct tcttcaagcc gttcgcggaa
      241 atattccatt caggtgcgtc cctgtctgtt tgcgatacga acgtaggttc tccgcctgac
      301 gttagacgaa cgttatgcga cattaaatct gcgtctacga cacgcccaac tccctgaatc
      361 ttcttccact gcaaatcggt gtagggttgc gaaacccgag gtgactcttc gattcgcgtg
      421 accttcattt cgaagctaaa atcggatttc gcaggctcga ccgcacccgt gatagcagct
      481 gcgtcttctg gattgggaga gcaggccaga ggaatatgac cctccgaagt aaagagcccg
      541 gaagttgcgt ccagaccaac ccagccagca ccaggcaagt acacttccgc ccaggcatgc
      601 aagtctgtaa aatcgtgcgc aggtccgggc ggtccagata gtggcgtttc atctggcttc
      661 agctgaatca aataaccgga aacaaatcgt gcggccaggc caaggccgcg caacacgtgc
      721 accagcaaat gagccgagtc acgacacgaa ccactcttca actttagagt atcttcgcat
      781 gtttgaactc ctggttccat gcgaatcaaa taggacacat cggaatgaat gcgctgattg
      841 agttctacca gaaagtcgat tgtcttttgc tttctacgat tggactccac atagtacttc
      901 agcagcggcc cgttttctct cagagcaagg taaggcgcga gctgtgcttt tacgtctgga
      961 gaatactgga agggaaactg ctccgcatct ggctcaagaa agaaatcaaa cggattgatg
     1021 gagtgaagtt ctgcgaccag atccaccgtg aaatccaggc gatccgtttt ctccggaaac
     1081 acaagcctgg ccaggtggtt tccaaaggga tcttgctgcc agttgatgaa atgaggttcc
     1141 ggcccgacgc gaaaggaata ggaaagaatg ggagtgcgtg aatggactgc gggcttgaga
     1201 cgcactacgt gaggaaatgc agtgactgca tgatcgtatc gatacgaagt cttatgatgt
     1261 aatgctacgc gaattgccat atatctgagc aagcacatcc gtgctcgcaa cagtagtgca
     1321 aagagcgtac cccgcctaac accttgcaga ggtcccgacc tggtcagaaa gcgaggatcc
     1381 gccaggtctg aacgattgcc tgccagaacg tccaatatgg cgttggtctg ggtttagaaa
     1441 ccgccatgcc gcaattccgt cggtccagcc cgtccgaaag caaattcgag agactaccgg
     1501 gaattcccga aacaaaacac gcaccttaac acgcatacta taaaaacgcg tattccgata
     1561 catgaattat atttcttatt ttttgattgc gtcatttatg ttttgtgttc cattaaaaat
     1621 ggaactgatc cacgctctgg caaccagcct gctctcacct atggttcgct gctctgtgtt
     1681 cgaagtcggt cttgtgaccg gacgcagaat gcaggatctg aaaaaagtcg ggtggttctt
     1741 gatcgccttt gcggtcgtta tgccgttgat tcatggattg ccgggtgtct tgattggaac
     1801 atggaccggt ctttcgatcg gaggaacttg cgttgccaga atctcccatc acctaccacc
     1861 tgacagcttc tattgtagtc acttttcctt ttgacgttgt ttgtggaatt cctttgtatt
     1921 acactctagc gggagccatc caatgaatct acaccacgta aaactgctga ccatcattgc
     1981 cccggacaat ctagaagcca gattgaccag tgacgttcgt ctcttcggag cacgcggata
     2041 tacgatttct cccgcggtag gagcaggact cagcagcgtg cgcgacaacg aatggactgg
     2101 aaaaaatatt cggcttgaga cgcttgtagg gcaagacgtt gcatacgctt tgatggcgca
     2161 cctggccaaa gattattttg aaaagttcgg agtgattgcg ttcatgtccg atgtagaggt
     2221 gcttcgcaaa gagcgatttg actaccgcta acgatccagc tctcgcgttc gattgagaat
     2281 ggaatcaatc tcaggcgatc ttgcatgtgc aactggtcgt ggtttatttc tgcgagacca
     2341 gacccacaaa agcgcaaatg ctcccacaat cgctaccaca atccagggcc acaccacggg
     2401 cggctttacg aagatcattt ttccaaaacc ttttacaaaa ccgctgacca gatcgactct
     2461 gccttctgcc tgcagaatga ttacttgagg atctttacga atttcttccc cgaaccctga
     2521 gacaagtcct tcttctatgc tgctttcgct tctgccttca atgatgcggt tttcaatgaa
     2581 ccgacgaagc tggactccga aacaattttc ttcgatgtgt ccgcatgttc ctatgatact
     2641 gtggcaacca cagtaacaat tcaccttcga cgtaagtcga ttgaacatcg caatctgagc
     2701 agaatcagta aggtgttcgg gagcacggcc ctgagcctgt gccgagctgg aaatcatcag
     2761 agccagaaac gcaatgccca cgcttctgac agaaaaaaga gccgagaaac tcatattcat
     2821 caaggtttca gaaactaccg tcgaaacaac caaaaataga tcgctgttag gcgcccgggt
     2881 atgtttgaaa ttgacgcaaa tctgactcgg ctaaaatgac aaaaatgggc ctgacggtcg
     2941 ccatcgttct tggactgctt cttgcggtct gcgcagcatg gattgcgcgc gagttacgca
     3001 atagacgcaa ccagatcgct cgtcagttgt tcctgagaca cggctccgtg atgcttctga
     3061 tcaaacctga cgacgggagg attgcgtttg cgaacctggc ggcggcccgc ttctacggct
     3121 attcaccgcg tcagctcgag tccatgtccg tcaaagacct gaacacgctg ggtccagcag
     3181 aagtccaggc tgaactggat cgcgcgcgaa gggaagagcg cagctacttc cttttccggc
     3241 atcgagttgc aagcggtgac attcgcacgg ttcaagttca ttcttctccg ctggtcatcg
     3301 atggagaaac ctttttgctc tccatcattg atgacgtgac ccaccacgcg aagctaacaa
     3361 aagagcgcga aaccagaggg cgcgttcttg aactgctcgc tgcaaacgct cccctggaat
     3421 ccatcctcac tgaacttgta ctggcttacg aagatctcgc gcccggttcc atttgctcta
     3481 ttcttctcac ggacagtgag gagagcacac ttcttcctgg tgccgctcct ggtcttcccg
     3541 aatggttcaa tcatgaagtg cagggactct ccacaggaga aggccaggga tcgtgcggaa
     3601 cggccatcgc gcgaaagcaa agagtcattg tttcaaacat cgcaacggat cctttgtggg
     3661 ataaatatcg ccatctcgca gaacgcgcag gccttgccgc atgctggtcc gaaccgattt
     3721 tttcaggatc aagaaaaatc ctgggatcat ttgcaattta ctacaaacag gttcgcgagc
     3781 cacacgaaga agatcttcaa ctcatctcca ggcacgcaca actggccagt ctctgcattg
     3841 aacaaacgca actccgcagg cgccagcaac tggccgatca cgtatttcaa aatacgggag
     3901 aaggaattct catcacggac gacaaaagtc agatccttgc ggcaaaccct gccttcggca
     3961 aactcactgg ctatgaacct gctgaaatcc ttggcaaaac acccagaatt ctttcctccg
     4021 gaaaacaccc gaaatcgttc tacgaagaca tgtggggaca gatcaggaac acaggcaaat
     4081 ggaagggaga aatctggaac aaacgtaaga atggagaact ttatcctgag ctgctgagca
     4141 tcagcacggt atacaccaaa ggatctgtca ttggatacta cgtggctata tttactgata
     4201 tctccaacat caaagaaaca gaggctcgcc ttgacgagct ggcgtaccgc gatccaatca
     4261 cagcgctcgc caatcgctcc ctgtttcaat tgcgactcgc agaagcattg catcgcgcag
     4321 aaagatcagg cacttctgtg tggatattgc tcattgacct ggatcgattt aaggatgtga
     4381 atgatgcgct cggtcacgaa gttggaaaca aactgcttga ggctgtaaca ggaagatttt
     4441 tagaagtgct cgaacccggg gacttgcttg cgcgcatggg aggagacgag ttcggcttgc
     4501 tccttgaaaa cgcagacgct ggaagaatcg cgcaacggct gctccattcg ctaaacgcac
     4561 ccgtccatct caaaagcgga gaccaaatcg atgtgggtgc aagtgtgggg atcagtcagt
     4621 ttccgcatga cggtaactct gttgaacagc tcatacagaa tgcggatgct gccctctatg
     4681 atgcaaaggc gtccggaaga agaacattcc gattttacac cagcacactc actgcccgcg
     4741 cgaaagccag actctctctc gaaacaaaac tgcgtcgtgc gctggaaaga aacgaaatcc
     4801 ttcttcacta ccagccgcag gtgaacattc gcacaggtaa aattgtagga gtagaagcac
     4861 tcgctcgatg gccggacggt gatcaaatga ttccaccgga tcaatttgtt ccggttgcag
     4921 aagaatcggg gctgattctc ccgctgggtc aatctctctt ccgaaaagca tgcatgcaag
     4981 cacgacaatg gaatgacgcg ggcatctctc tcatggtggc tgtgaacctt tctgccaggc
     5041 aatgtgcaaa cgctcgtctc gcgcacgaaa tcgcggaagt tattcgcgag acaggagtga
     5101 atccagaaca actcgaactt gaaattactg aaactgctct gatgagtcag gggcaagatg
     5161 cgctcaatct gctggaacaa atgagaaaga ttggagtgcg catggctcta gacgattttg
     5221 gaacaggcta ttcttctctg gcctacctcc gtcactttcc agtcgatact ttgaagattg
     5281 accgcagttt tatcagcgaa atccccaccc acaacagtgg tgctgaaatc acatccgcca
     5341 tcattcgcct cgggcattcg ttgaacttca cagtgctcgc agaaggcgta gaaacagaag
     5401 cacagctcgc gttccttgca aaacaagaat gcgacctcta ccagggctac taccgcggac
     5461 gagccgtccc gccggaagaa atcccggctc ttctgcaata aacccatcga atacttagtg
     5521 cttgacaaac ctaacaccgt tagttttatt gtccccagac agctaacact gttaggagta
     5581 attatggaac cgagactcac tttaccggcc gttcgggata cgatctcgaa agaatctttg
     5641 cgtaatccca catggaaagg attgctatac tttgcacgag acatcgcagt ctacgctgcc
     5701 attctggttg gactctattc gacagacgcc tggtatttcc tggtgcctct gtggattctg
     5761 gcaggcctgg ccgtgagcgg ccttttcatc atcggtcatg acgccgcgca cggggcactt
     5821 ttcaaaaatc agaagctggc gtatttgatc ggacaaatat ccatgctacc ttccctgcac
     5881 gcgtatcacc agtgggtata cggacacaat cggattcacc atggccatac catcaagatg
     5941 gaaggcgact ttgtctggcg cccaacttcc cccgctcagt atcgcgacct ttcctgggca
     6001 ggaaagattt ggcacagaat cacatggtct gcgatcggct ctggcatcta ctatctagtt
     6061 caaatctggt ttggaggaat gatcctgttc gtcgcgcaga cgaaaggcgc aaagcgagat
     6121 agaattctaa tcattctggc agcaatcgcg atgattgccg ttccaatcgc actgtttcca
     6181 acctggcagg caggcctctg ggtggccgtg aagctgggcg tcattccatt tcttgcattc
     6241 aactactgca ttggattctc tgtctatgtt catcatatcc atgacttcat tccatggaag
     6301 aagcacaaag actggacgcc gttctacgga cagatgcttg gaactgtgaa ctaccacatt
     6361 cctgctttct tcaatttctt cttacacaac attttcctgc acatgcctca ccacgtgaat
     6421 atgcgcgtgc ctttctacaa ccttggacgg gccttgagcg agatcaaatc ggtgtatgga
     6481 caatatgtga ttgaaagaaa atcgatgttc atggactatc tacacactac cagaaagtgc
     6541 aaactgttcg acccagaaag cggaaagtgg ctaacgtacg cggaagcggc ggaagaaaga
     6601 acgccaaacc tcagccagtc cgtgccggcc tgagccggga atctatcctg gatgccgccc
     6661 tcgaaattct aaacgagggc ggtatggccg ctctcagcct tcgcagcatc gtcaagagac
     6721 tgggctgcag cgtcgcagga ccttacacat atttcgcggg acaggatgaa atcatcagca
     6781 ctctcatcca gagaggagaa gaggaactca caggagacct caaacgcgca ggccaggaag
     6841 gcggagatct tttcgaagtc ctaagaagac tggccgccac atacatgaca ttcgcgcgac
     6901 gcaaccgcga gctgcacaaa ctcatgttta acatcaatgg acacaaacgc atttttcctt
     6961 cccaaccgca agctatcgcg cctatattga aaccatccgg ggtggagtaa aaagtggcag
     7021 acttcatttt tcacgcaaag gctaccacgc catagcgcga actatgtggg cctggctcta
     7081 cggattgatt gttctagaaa tgaccggcgt taccacgatg aatgcagatg aagcaatcga
     7141 cgaaggattc agctttttcc agcgcttgct ggagcaggga gagaaaagcg cctaacgggg
     7201 ctgcggattc actacaacca gtgcctttgt ttctcgactc aaaaactgat ctaaatctgt
     7261 cttgctcaat ccgaattcgt agaaagcatg tgacgcctga tccgcgtacc agaagtagtt
     7321 catgggcctg cccagttcca cttccgggta ttcgagagtc ttaaagaaat atttagaa
//
LOCUS       DAOQJL010000143         7338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5594_length_7338_cov_5.068653, whole genome shotgun sequence.
ACCESSION   DAOQJL010000143 DAOQJL010000000
VERSION     DAOQJL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5594_length_7338_cov_5.068653"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2208)
                     /gene="nirB"
                     /locus_tag="PLG78_08085"
     CDS             complement(<1..2208)
                     /gene="nirB"
                     /locus_tag="PLG78_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNE23112.1"
                     /translation="MKRKVIVVGHGMVAHRFIEKLSSATDVEITVFGEEKYPAYDRVH
                     LSSFFDTNSHEGLLLADPAAYAEKGIQLKTGVRVDSIDPAARTIVDSNGTSYPYDILV
                     LATGSTPFVPPLPGRQKTGVFVYRTIDDVMAMRDFSKKATSAAVIGGGLLGLEAAKAS
                     LDMGLKTHVIEFAPRLMPRQIDEDGSSFLCDKIKSLGIELHLGAEVGEIHGDDSVCGV
                     GLKSGEQLSVEMVIISAGIRPRDDLAKSCGLTVGERGGIAVDDSMRTSDPNIFAIGEA
                     ALHRGFVYGLVAPGYEMAAVAADAIVGGERQFTGYDLSTSLKLVGVDVASFGDPFGNK
                     ETKPIVFADRYSGVYKRLNLSADGRRLLGGILVGDASDYGMLTQFTSAGTVLPEDASA
                     LLLGGKSAKMPELAGTAEICKCNNVSKDTIVAAVTAGCTELGALKKQTCAGTGCGGCS
                     PMVSEILNKTLKDLGQTVDMRLCEHFPHTRQELVHIIQIRQLKSFREVLSACGQGVGC
                     EICKPAVASILASSWNDLILRDDVVQDTNDRFLANIQKGGTYSVIPRIPGGEITPERL
                     IVIGQVAKKYNLYCKITGGQRIDLLGARVDQLPQIWEELIDAGFESGHAYGKALRTVK
                     SCVGSTWCRFGVQDSTSFAIRIEERYKGIRAPHKLKSAVSGCIRECAEAQSKDFGLIA
                     TEKGWNLYVCGNGGARPQHAVLLASDISEDLAIKYLDRFIVYYIRTAERLTRTS"
     gene            2513..3943
                     /locus_tag="PLG78_08090"
     CDS             2513..3943
                     /locus_tag="PLG78_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNE23113.1"
                     /translation="MSREAITMQSESTLEFKMPEVKTNPRTIEHWDVENESFWKTTGR
                     AIALRNLAFSIFAEFLAFTIWQVWSAVATQLPAVGFKFTLNELFWLAALPGLTGATLR
                     MPYAFMVPIVGGRNWTLISTALLLFPAVGIVMAVQNPETPYWVFLTLALLCGFGGGNF
                     ASSMANINFFFPKRRKGFALGLNAAGGNIGVSVVQFVTPLVIGLSIFGSFGGSGQMAG
                     TKTLFLQNAALIWIPFIVIAFGTTWFFMNNLHVSRSSVKEQLTMLKDKNAWIMSLLYM
                     GTFGSFIGYSAGFPLLIKSQFPNVNPLQFAFIGPLVGSVIRPVGGWLGDKIGGARVTF
                     WNFAVMGLAVSGVIYFLSIKTEPGAFYGFFAMFLILFVAAGIGNGSTYRMIPIIYMRK
                     NNAHEGATATTESLNRANRQSGVVVGITSAIAAYGAFFVPKSYGTSIELTGGPSGALI
                     LFLAFYIGCLAATYYFYYRKKAAYPC"
     gene            3937..>7338
                     /locus_tag="PLG78_08095"
     CDS             3937..>7338
                     /locus_tag="PLG78_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE23114.1"
                     /translation="MLSGTRTTCSYCGVGCGIVVREHKGTLQVEGDPEHPVNRGMLCS
                     KGRTLGYTIADKTDRILYPRLRASRGHSQSRVTWDTALNRAANVFRSITEKYGPDSVG
                     FYVSGQLLTEEYYIVNKLTKGFIGTNNIDTNSRLCMSSAVTAQKMAFGEDCVPINYED
                     IELADLYLIAGANPAWCHPILFRRMEEKGERVRKVVLIDPRKTDSASLADLHLQLRPG
                     TDVALYNAIARILIEKGNIDSNFISNHTENYSTLEEAALSISVREAAEICDLSASDIE
                     KCADWIADSSGFLSFWAMGLNQSAQGVRKNLSLINLHLITGHIGKPGSGPFSLTGQPN
                     AMGGREVGGLATMLAAHRDIGNQAHREEVKTFWNSKDISPKPGRTATEMFDALRSGEM
                     KAIWIACTNPIVSLPDSMAVQEGLEKARFVVVQDISERSETVKYADLVLPAAGWLEKE
                     GTMTNSERRITYLPKLIEPPGEARPDSEIWGDFARRMGYADAFAYKSAEEIYAEHCAL
                     TRGTNIDVSGLSYDRLKQSSMQWPVPHPDHPGTPRLFQDLKFHTQSGRAKIHGFRFEN
                     RSEALSAEYPLVLTSGRIRDQWHTMTRTGKVNKLGTHIPEPYVEINPEDALSRGIAEG
                     DLAEVHSKRGSVRLRARLDERIRPGLVFVPMHWGSTIGDDGTRANNVTSPALDDFSKQ
                     PDLKYAAVQVRKPVFTRRKVVIIGAGASAFRFLEEYRRMNQTDDITLICAESESFYNR
                     ILLPDVISGRKEKDSLSLVDAAQVTEWKLKLATETRATSIDRISRTVLDSRGQSHPYD
                     ILVLATGSRAILPPRLAGLSGVHTLRKLADAEQILADAKGPVVVLGGGVLGLETAGAL
                     SERGLSVTVIHRSSALMSRQVDATTGRLLRSAMIDRGIQTIVNDSIAACRGVDRIDSV
                     RLTSGRIVDCETLICAAGTEPDIDLARDAGLACESGVCVDDRMQSSDPHIYAIGEAAE
                     HRGTLYGTTLAAEDQGRTAARALCGDPGAVYSGSIRMNILKVEGVRLASIGRVEASHP
                     GQEELVYTDESAGIYKKCVVEGDRLIGCILFGDLSEMEQFRALIASRTELGELRRSLL
                     MRFEEIAPLKGDLVCSCNSVGKGNIEDCISAGITDFKELTAKCKAG"
BASE COUNT         1866 a         1950 c         1835 g         1687 t
ORIGIN      
        1 actggtcctc gtgagtctct ccgcagttcg tatgtagtac acaatgaatc gatccagata
       61 tttgatagcc agatcttcgc taatgtcaga cgccagaaga actgcgtgtt gcggacgcgc
      121 tccaccattt ccgcaaacat acaggttcca acccttctct gtagcgatca aaccaaagtc
      181 tttactttgc gcttctgcac attcgcgaat gcagccggaa acggccgact tgagtttgtg
      241 cggcgcgcga atccctttgt aacgctcctc gattcgaatc gcaaagctcg ttgaatcctg
      301 gactccaaag cgacaccagg tagacccaac gcaactcttc actgtgcgaa gagccttgcc
      361 gtacgcgtgc ccgctttcaa agccagcatc aatcaattcc tcccatatct gaggaagttg
      421 atctacgcgt gcgcccagaa ggtcaatcct ctgtccgcct gtgatcttgc agtatagatt
      481 gtatttcttt gcaacctgtc caatgacaat caagcgttcc ggtgtgatct ctccaccagg
      541 aattcgcgga atcacagaat aagtgccgcc tttctgaatg tttgccagaa aacgatcgtt
      601 tgtgtcctga actacatcat cgcgcaaaat caaatcgttc caggaacttg cgagaatgga
      661 tgcaacggcc ggtttgcaga tttcgcatcc aactccctgg ccacaggcac tgagaacttc
      721 cctgaaagat ttcaactgtc tgatctgaat gatgtgaacg agctcttgtc tggtgtgtgg
      781 aaaatgttca cacaacctca tgtccaccgt ctgacccaga tcctttagag tcttgtttag
      841 aatttcagaa accataggcg aacatccacc gcatccagta ccggcgcatg tctgtttctt
      901 aagggcaccc agctccgtac aacctgctgt aacagcagcc acgattgtgt ccttgctcac
      961 attattacat ttgcagattt cagccgtccc ggcaagctcc ggcattttcg cggattttcc
     1021 gcctaatagc agtgcagacg catcttccgg aagaactgtc ccggcagaag tgaattgagt
     1081 gagcatgccg tagtcggatg catcacccac tagaatgccg cctagaagcc tgcgaccgtc
     1141 cgcggacaga ttcaaacgtt tgtatacacc agaatagcga tctgcaaaaa caattggttt
     1201 cgtttcttta ttaccgaatg gatctccaaa gctcgcaacg tctacgccca ccagtttcag
     1261 gctcgtggat aaatcatagc cagtgaattg ccgctcaccg cccacaatag cgtctgctgc
     1321 cactgccgcc atttcatatc caggagccac gagaccatac acaaaacctc tgtgcagagc
     1381 cgcctcgcca atcgcgaaga tgtttggatc agaagtgcgc atggagtcat ccactgcaat
     1441 gccacctcgt tctccgacag tcagaccgca acttttcgca agatcgtccc ggggacgaat
     1501 ccccgcggag ataatcacca tttcaacaga aagctgctca ccgcttttga gtccaactcc
     1561 gcatacagaa tcgtctccgt gaatttcccc gacttccgca ccgagatgca attcaattcc
     1621 aagcgatttg attttgtcgc agaggaaaga agaaccatct tcatcaatct gtctgggcat
     1681 aagcctgggt gcaaattcaa tgacatgcgt tttcaggccc atatccagcg aagccttcgc
     1741 agcttcgaga ccaagaaggc cgccaccgat gacagccgca cttgtggcct tcttactgaa
     1801 atcgcgcatg gccatgacat catcaatcgt gcgataaaca aacactcctg tcttctgtct
     1861 tcctggaaga ggtggaacga aaggagtgga acccgtcgca agaaccagga tatcgtaggg
     1921 ataagaagta ccgtttgaat ctacgatcgt cctggccgcg ggatcaatgg aatccacgcg
     1981 aacgccagtc ttcagttgaa ttcctttttc tgcataggca gcagggtcag ccaggaggag
     2041 cccctcgtga gagttcgtat caaagaaact ggaaaggtgc actcgatcgt acgctggata
     2101 tttttcctca ccgaatactg tgatctctac gtccgtggca gaagagagct tctctataaa
     2161 ccgatgagcc accatcccgt gaccgactac tatcaccttg cgtttcatgg tttatctaga
     2221 gcaataagca taccaatgac agaagcaggt caaagcggat agtttgggac acaatccgcc
     2281 cacataacag gcaattatat gaatccgtga aataaccgcc cgattttata ccctagtgcc
     2341 tcaagtttac acacactgcg acccgctcgg caatttgcag aattcgtccg acgttctgtt
     2401 gatctttgcg tctttcttcc attaaaggaa ggtgttaggc gaaaatacac tgcggccagc
     2461 catgccagca gaacaaaaca tcacgtggca ccgttcttgc tgaaggcatc ccatgtcgag
     2521 agaagcaatt accatgcaga gcgaatcaac gctcgaattc aaaatgcccg aagtgaaaac
     2581 aaacccacgc accattgaac actgggatgt ggaaaatgaa tccttttgga aaactacggg
     2641 ccgcgccatt gctctgcgca accttgcgtt ctccatcttc gcagagttcc tggccttcac
     2701 aatttggcag gtgtggagtg cggtagctac acagctccct gcagtgggat tcaagttcac
     2761 tctgaatgaa ctgttctggc tcgcggcact gcctggtcta accggtgcca ctttgcgcat
     2821 gccgtatgcc ttcatggttc cgatcgttgg cggcagaaac tggactttga tttcaaccgc
     2881 gttgcttcta tttccggcag tcggcattgt gatggcggtg cagaatccag agactcccta
     2941 ctgggtcttc ctcactcttg cacttctgtg cggatttggc ggaggtaact tcgcttcaag
     3001 catggcaaac atcaacttct tctttcctaa aagaagaaaa ggatttgccc tgggtctcaa
     3061 cgcagccggc ggaaacattg gagtgagtgt agtgcagttt gtcactcctc ttgtgattgg
     3121 tctttctatc tttggatctt ttggcggttc aggccagatg gcaggcacga aaactctgtt
     3181 cctgcaaaac gcagccctca tctggattcc gtttatcgtc atcgcgttcg gtaccacatg
     3241 gttcttcatg aacaatctac acgttagccg ctcttcagtg aaagaacagc tgaccatgct
     3301 caaagacaaa aacgcctgga tcatgagtct tctctacatg ggaacattcg gatctttcat
     3361 tggatattct gcaggatttc ctctcctcat caaatcgcaa ttccccaacg tcaatcctct
     3421 ccagttcgca ttcattggtc cactggttgg atccgtgatt cgtcccgtag gcggatggct
     3481 gggagacaag attggaggcg cgcgcgtcac cttctggaat ttcgcagtga tgggcctcgc
     3541 agtgagtggc gtaatctatt tcttgagcat caagactgaa ccaggtgcat tctacgggtt
     3601 ctttgcgatg ttcctcattc tctttgtggc ggcgggaatt ggaaacggct ccacgtatcg
     3661 catgattcct atcatctaca tgagaaagaa caatgcgcat gaaggagcaa ccgcaaccac
     3721 cgaaagtctc aaccgcgcaa accgtcagtc cggagtggtg gtcggaatta catcggccat
     3781 cgcagcatac ggagcattct ttgtacctaa aagctatgga acttccattg aactgaccgg
     3841 cgggccttcc ggagcgctca ttctcttcct cgcattctac attggatgtc tggcggcgac
     3901 atactacttc tactatcgta agaaagcagc atacccatgc tgagtggcac gcggactacc
     3961 tgttcctact gcggcgttgg ctgcggaatt gtagtccgcg agcacaaagg caccttacaa
     4021 gtagaaggtg atccagagca tcctgtcaac cgggggatgc tctgctccaa gggccgcact
     4081 ctcggttaca caattgcgga caaaacagat cgtatcctct atccgcgcct cagggcgagt
     4141 cgtggtcatt ctcaaagtcg tgtaacgtgg gacacagcgc tcaatcgcgc agcaaatgtt
     4201 tttagatcca tcaccgaaaa atacggcccg gacagcgttg gtttctatgt atccggccag
     4261 cttctcacag aggaatacta catcgtaaac aagctcacca aaggattcat tgggacgaac
     4321 aacatagata cgaattcgcg cctttgcatg agttcggctg tgacagcaca gaagatggca
     4381 ttcggagaag attgcgtacc tatcaactat gaagacattg aactggcaga tctatacctg
     4441 atcgcaggag ccaatccggc atggtgtcat ccaattctat ttcgcagaat ggaggagaag
     4501 ggagagcgag ttcgaaaagt agtcttgatt gatccaagaa aaacggattc ggcctccctg
     4561 gcagatcttc atcttcagct tcgcccggga acagacgtcg cactctacaa tgcgatcgca
     4621 cgaattctaa tagaaaaagg gaacatagac tcgaacttca tttccaatca tacggagaac
     4681 tattccactc tcgaagaagc ggcgctttcg atcagcgttc gcgaagcggc ggaaatatgc
     4741 gacctgtccg cgtctgatat tgagaaatgc gcggactgga ttgcagactc cagcggattc
     4801 ctgagcttct gggccatggg actcaaccag agcgcgcagg gagttcgcaa gaatctatct
     4861 ctcatcaatc ttcatttgat cacaggtcac atcggcaagc ctggatccgg cccgttctct
     4921 cttacaggtc aaccaaatgc gatgggtgga cgcgaagtcg gcggactggc caccatgctc
     4981 gccgctcatc gcgacattgg aaaccaggca catcgcgaag aagtgaaaac cttttggaat
     5041 tcgaaagaca tttccccgaa gccgggcaga accgcgacgg agatgtttga tgctctgcga
     5101 agcggagaga tgaaagcgat ctggattgca tgcacaaatc cgatcgttag ccttccggac
     5161 tccatggcag tgcaggaagg acttgagaaa gcaagatttg tggtggtgca ggatatttct
     5221 gaaagatcag aaacagtcaa atatgcagat ctcgttctcc ctgcggcagg ctggctggaa
     5281 aaagaaggaa cgatgaccaa ctctgaacgc cgcataacgt atcttccaaa gctcatcgaa
     5341 ccgccggggg aagcgagacc tgatagcgaa atctgggggg actttgcacg tagaatgggt
     5401 tatgcagatg cattcgcata caaaagcgcc gaagaaatct acgcggagca ctgcgccctc
     5461 actcgaggaa caaacataga cgtttccgga ctatcctacg atcgactcaa acaatccagc
     5521 atgcaatggc ccgttcctca tccggatcat ccgggcactc cgcgcctctt tcaagacctg
     5581 aaattccaca cacagagcgg tcgcgcgaag atccatggat ttcgatttga aaatcgttca
     5641 gaagcactca gcgcagagta cccgctggtg ctcacatctg gaagaatccg agatcaatgg
     5701 cacacaatga cccgaacggg aaaagtaaac aaactgggca ctcatatccc agaaccgtat
     5761 gtagagatca accccgaaga cgctctttcg cgtggcattg cggaagggga tctggcagaa
     5821 gttcacagca aaagaggaag cgtcaggctc agagcccgcc tggatgaaag aattcgacct
     5881 gggctcgtat ttgttcccat gcactgggga agcacgattg gagacgatgg cacaagggcg
     5941 aataacgtta cttctcccgc cctggatgac ttctcaaaac agcctgatct aaaatatgcc
     6001 gcagtgcagg tgcgaaagcc agtctttacc aggcgaaaag tggtaatcat tggcgcaggc
     6061 gcatccgcat ttcgcttcct cgaagaatac aggagaatga accagacaga tgacatcact
     6121 ctgatctgcg cggagtcgga atctttttac aatcgaatcc tccttccgga tgttatctcc
     6181 ggccgaaaag aaaaggatag tctgtcgctt gtggacgcgg cgcaagtgac tgaatggaaa
     6241 ctcaagctcg cgacagaaac gcgggccact tccatagatc ggatttctcg caccgttctc
     6301 gattcgaggg gtcaatcgca tccgtacgac attcttgttc tcgcaacagg cagtcgggca
     6361 attctacctc cacgcctggc cggactcagt ggagtgcaca ctcttcgcaa gcttgcggac
     6421 gcagagcaaa tcctggcaga tgcaaagggc ccggtcgtgg tactcggtgg aggagtcctg
     6481 ggcctggaaa cggcgggagc actcagcgaa cgcgggcttt ccgtgaccgt cattcatcgt
     6541 agctctgcgc tcatgagcag gcaggtggac gcaaccaccg gtcggcttct tagaagcgcc
     6601 atgatagatc gcggaatcca gacgatcgtc aatgatagca ttgctgcatg tcgcggtgtg
     6661 gacagaatcg attcagtcag gttgacgagc ggacgaattg tagactgcga gactttgatc
     6721 tgtgctgcgg gcaccgaacc tgacattgat ctggcgcggg atgcgggact ggcgtgcgaa
     6781 agcggagtgt gcgtggatga ccgcatgcag agctcagatc cgcacattta cgccatcgga
     6841 gaggcggcgg agcatcgcgg aacgttatac ggcactactc ttgcggcgga agatcagggg
     6901 cgcacggctg cgcgcgcgct ctgcggggat ccaggggccg tttacagcgg atcaatccga
     6961 atgaacattc tcaaagtgga aggagttcga ctcgcaagca tcggccgcgt ggaagcttca
     7021 catcctggcc aggaagaact ggtctatacg gatgaatccg ccggcattta caagaagtgc
     7081 gtcgtggagg gcgatcgtct cataggctgc atcctgttcg gcgatttgag cgaaatggaa
     7141 cagtttcgcg ctctcattgc atctcgcacg gaactcgggg aattgcgacg ctctctcttg
     7201 atgcgatttg aagaaatcgc cccactgaaa ggagatctag tatgctcctg caattccgta
     7261 ggaaaaggaa acatcgaaga ctgtatctct gccggaatca ccgactttaa agagctcact
     7321 gcaaaatgta aggctgga
//
LOCUS       DAOQJL010000144         7284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5638_length_7284_cov_4.185779, whole genome shotgun sequence.
ACCESSION   DAOQJL010000144 DAOQJL010000000
VERSION     DAOQJL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_7284_cov_4.185779"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLG78_08100"
     CDS             <1..864
                     /locus_tag="PLG78_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23115.1"
                     /translation="IPSATGNTRRQIEMIQDFGVTAIHCTPSYALHLAEAAEEMDADL
                     SSLKKGMFGAEPWSDAMRKDLEKKLGVDPGGRNLLRRFLHFGKVLLERAEQQYGVEVS
                     PDKDFDYRIGRIRHTILDNIADRFNVKNFDRKADAIGKLRQLFALMEMISIGYPDPNL
                     PKVTPEDLEWAHGECVKAFDFIVIKKDYLVSNPTPERFYEWLARFESYVYGKKPRALG
                     GEPSPRARKAVVKFAKPFRLSEFAPKDKKAKKSALDELLKKLRREMQEMLDESQSLTR
                     PLFKPGDIGGE"
     gene            893..1180
                     /locus_tag="PLG78_08105"
     CDS             893..1180
                     /locus_tag="PLG78_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNE23116.1"
                     /translation="MYTFRAKVWIYSGQGAWHFVTLPAKTARELKEDYGHSRRGWGTI
                     KVTASTGKTKWNTSVFPEKKSGSFVLPIKMEVRKAEKISAGKTIQVHLEIA"
     gene            1276..1860
                     /locus_tag="PLG78_08110"
     CDS             1276..1860
                     /locus_tag="PLG78_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23117.1"
                     /translation="MIAGHYAAAFLPAAHKARGPFWLFLLASQIPEFLWLLFAIAGIE
                     PTLPASILDATFDSIVVDMRFSHNLVPALFQAIITGVVVAIVFRSAVTALWSAALVVI
                     HVLCDYVVGFSHQIMWYSSPSIGLNSYKTMPYVAIGIELVFAWICLGYFYWKRKEDLP
                     WSNRQWMILTVAFSAGILAWMPAARIPIRHWLGL"
     gene            1901..3976
                     /locus_tag="PLG78_08115"
     CDS             1901..3976
                     /locus_tag="PLG78_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23118.1"
                     /translation="MRQIARQLILIAALIATAGCSHIFPPQGPIQLNESTPMFRPGPQ
                     MEYCVDESGTKTLEEIKNCNFIPVRAYYPAFGITTNAVWVRFRILPDPKATTSWYFEL
                     ASSLPRSVHFYRLHQDGTLADKLIYDEVPPRLRLIPHHYFLFPIQNVTEPESIYMRAQ
                     SVGGLNMPVFIWNARSFPMRDMLRTGFFAMFFGLMFAMALYNLLLFISIRDRSYLYYV
                     FYILFVTEYFAFISGHAAYLVPDSWTGKTPALGPVVALAATIFALIFSRKFLLLPEKR
                     PRLAMAANVVALIHLLGLIALPFLTIPQMAQIGNGMPVLGIIVILVAAISLVRDGFKP
                     AKYFLTAWSFLMIGILLFIVQNLNFIPAGFITQYGQFFGAAFEAILLSLALGYRINSL
                     RQSEQEARRDLSQEQARALAEQTAAAESFARFVPGEFLEYLGKKNIMSISHGDAVRKQ
                     MAVLFLDIRGFTEMSERLGSEGTFSFLNEFHGGLEPIIQKHDGFIDKFIGDAIMALFP
                     APTGAVQAAIEMQNWVAYTGKARVGIGIHFGELMLGTIGSPHRLETTVIGDTVNLASR
                     IESINKQYGTEIIVSDSVYKLIQEIPQLAAREVDAIRVRGKKQPVVLYEIMNGLSPDV
                     LASREKHISSYMGALLSYRAGDFAAAKTQFSEHMQNVNGDSVAEMYLKRLDRINESAP
                     WDGIWEAEH"
     gene            complement(3994..5076)
                     /locus_tag="PLG78_08120"
     CDS             complement(3994..5076)
                     /locus_tag="PLG78_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNE23119.1"
                     /translation="MELLVIVGFSASCKYPSHCESVFCSIWPAALVFARPRTLPDTGQ
                     TTCATTAGAVISCAGTGQDAEYATPGAYILYETTLGNAVGEVLGSDLLWQKCSFGQTG
                     NSCAGTALSTVTRTQAFAACSGVAIADRVWRLPSVREMHLLPYFGGATVAMPSSFFPN
                     TASGTNVYHWTSTTVVASPANSYLFAQKFGFFASQSNALATDTFARCVAGPAQPTQDY
                     VRLGVTEPSVRDNTTGLTWTVCALKTGGAADTSANCAGPAPANFTQTNALTFCESLVY
                     GGFTDWRLPNVRELASLMDYTQSVNPTVNQTYFTANPSATVYWSSTNDISNAANAYIF
                     DVSDGTSGTIARTASIGDVRCVRGPW"
     gene            complement(5232..5795)
                     /locus_tag="PLG78_08125"
     CDS             complement(5232..5795)
                     /locus_tag="PLG78_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE23120.1"
                     /translation="MQRSWFARFFALSVGLTKSGSGANNDLKDRRFMKLSKKTDYALR
                     AIFTLVEQHATGEPVSISDLAQANRIPKRFLEHIMLDMKSQGWVSSTAGKYGGYRLAK
                     HPSQITMGEIVRYFDGILAPIGCVSIARYEPCKQERVCRFRRLLLNVRNAVAGMMDRA
                     TLESVFAEGIVTQKEVMHVSLMGGDGI"
     gene            complement(5758..>7284)
                     /locus_tag="PLG78_08130"
     CDS             complement(5758..>7284)
                     /locus_tag="PLG78_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE23121.1"
                     /translation="RNCPGSPMGETNAQFRQILDVSPIPFALNDENQNITYLNRAFTR
                     TFGYDLTDIPTLAEWWPRAYPEPAYRERVMKSWQERMEKATREGTQFEALELTIRCKD
                     GSDRIVIVSAVSPGDAPEGVYAVMLYDITERMNLLAAAQRADRLNSLGVLAGGIAHDF
                     NNLLSSIFGYMELARGATTESRVIEYLDRGLSAFSRARGLAQQLLTFARGGDPVMQSG
                     NLGTLLSETCEFVLAGSNVHYQCHVADDLWPCNFDTGQMNQVFSNLLINARQAMPDGG
                     NIGAVASNRSLNETSVHPAGPYVEIQISDSGRGIPAEQISHIFDPFYSTRPGGTGLGL
                     ATCFSIIQKHAGFIDVESTPGKGATFRILIPVAKSGNSRGASFAKHSAGEQRLLVMDD
                     EELIRAVSQDLFASAGFSVQVAAHGEEALILCDEAARSGRPFHAAILDLTVPGHMGGK
                     DLARLLRKDHPEIALFASSGYSEDPVMTAPAEYGFVASIRKPYLIADAAQLVRQVLRT
                     "
BASE COUNT         1831 a         1885 c         1806 g         1762 t
ORIGIN      
        1 atcccgagtg ctaccggtaa cacccgccgg cagatcgaga tgatccagga cttcggcgtc
       61 accgcaatcc actgcactcc cagttatgca ctccaccttg cagaagctgc cgaggagatg
      121 gatgctgatc tttccagcct gaagaaagga atgttcggtg ctgaaccgtg gtcagacgcc
      181 atgcgaaagg atcttgaaaa gaaactcgga gtggatccgg gtggaagaaa tctgcttcgc
      241 cgattcttgc atttcggaaa agttctactc gagcgcgcgg agcaacagta cggagtggaa
      301 gtttctccgg acaaagattt cgactatcgc attggaagaa ttcgccacac gattctggac
      361 aacattgcag atcgattcaa cgttaagaac tttgatcgca aagcggatgc aatcggaaag
      421 ctgcgtcagc tattcgcgct catggaaatg atatccattg gttatccaga tcctaatctt
      481 cctaaagtaa ccccggaaga tctggaatgg gcacatggtg aatgcgtaaa ggctttcgac
      541 ttcatcgtca tcaagaaaga ctacctggtt tctaacccta caccggagcg tttttatgaa
      601 tggctggcac gatttgaatc ctacgtctac ggcaaaaagc cgcgcgcact cggaggagag
      661 ccatctccgc gcgctagaaa ggcggtcgtg aaatttgcaa aaccgttccg cctctcggaa
      721 ttcgcgccaa aagacaagaa ggcaaagaaa tctgctctag acgaattgct caagaagctc
      781 cgccgggaaa tgcaagaaat gctcgatgaa tcacagtcat tgacccgtcc tcttttcaag
      841 ccaggagaca ttggaggaga gtgacggata ggatcaaccc ttcgcgccag ttatgtacac
      901 atttcgcgcg aaagtatgga tctactccgg gcagggagcg tggcattttg ttacactccc
      961 tgcaaagaca gccagagaac tgaaagaaga ctacggccac agcaggcgcg gatggggaac
     1021 catcaaagtg actgcgtcga cgggaaaaac aaagtggaac acatctgtct ttccagagaa
     1081 aaagagtgga tcgttcgttc tgcccattaa aatggaagtg cgcaaagcgg aaaaaatctc
     1141 cgctggcaaa accattcagg tacatttaga aatcgcttaa cggtaaggag catccatgac
     1201 gtccctcatt tttgcgctca ttgcgttcta cgcgttcttt ttctggctgc ttatgcggct
     1261 tgcaaggcgg agcgtatgat cgccggtcac tacgcggctg cttttcttcc cgcagctcac
     1321 aaagcacgag gaccgttctg gcttttcttg cttgcttctc agattcctga atttctctgg
     1381 ctgctttttg cgatagccgg cattgagccc acgctccctg catccattct ggatgcaacg
     1441 tttgatagca ttgtagtaga tatgaggttc agtcacaatc tggttcccgc cctcttccag
     1501 gcaatcatca ctggagtcgt ggtggccatt gtgtttcgaa gcgcagtcac tgctctctgg
     1561 agcgccgctc tcgtcgtcat acatgtgcta tgcgactatg tggtcgggtt ttcgcatcag
     1621 atcatgtggt attcttctcc atccattggt ctaaacagct acaagacaat gccctacgtg
     1681 gccattggaa tcgagctggt gttcgcctgg atttgccttg gttacttcta ctggaaaaga
     1741 aaggaagacc tcccatggtc gaacaggcag tggatgattc tgactgttgc ttttagcgcg
     1801 ggcattctgg cgtggatgcc ggctgctcga atccccattc gacactggct tggtctgtag
     1861 aagtcatttg acgaacaccc acagccgttt gtactgcgac gtgcgacaga ttgccaggca
     1921 actgattctc atcgcggcgt tgattgcgac agccggctgc tcacatatct ttccgccgca
     1981 gggccccatt caattgaatg aaagtacgcc catgttcagg cccgggccac agatggaata
     2041 ttgcgtggac gaatctggca caaaaactct ggaagagata aagaattgca actttattcc
     2101 tgtgcgtgct tactaccctg ctttcggaat cacaaccaac gcagtctggg tccgctttcg
     2161 cattcttccg gatccaaaag caaccaccag ttggtacttt gaactggcca gttcacttcc
     2221 gcgctcagtt catttctatc gacttcacca ggatgggacc cttgcagata agctcattta
     2281 cgatgaagtt cctcccaggc tcagactcat tccgcatcat tactttctgt ttcccatcca
     2341 gaacgtaaca gaaccagagt ccatctacat gcgcgcgcag tctgtgggcg gactcaatat
     2401 gccggtcttc atatggaatg ccagatcctt tcccatgcgc gacatgctac gcactggttt
     2461 ttttgccatg ttctttggcc tcatgtttgc catggcactc tacaacctat tgctatttat
     2521 atccattcga gacagaagct atctctacta tgtcttttac attttgttcg taacagagta
     2581 ttttgcattc atcagcggtc atgcggcata cctggtgccc gattcatgga ctggaaaaac
     2641 accggcgctt ggaccggttg tggcgctggc tgctaccata ttcgctctca tattctcgcg
     2701 aaaatttctg ctcctgccgg aaaagaggcc tcgcctggca atggcggcta acgttgtggc
     2761 tttgattcac cttctgggtt tgatcgccct tccattcttg acgattcccc agatggcgca
     2821 gattggcaac ggaatgccag tcctcgggat cattgtgatt ctggttgctg cgatcagcct
     2881 ggtgcgagat ggatttaagc cagccaaata cttccttact gcatggtcgt tcttgatgat
     2941 tggaattttg cttttcatcg tgcaaaatct aaacttcatt cctgcaggct tcatcactca
     3001 atatggacaa ttttttggag ccgcgtttga agcgatcctt ttgagtctgg cgctgggata
     3061 cagaatcaac tcattgcgac aatcggaaca ggaagccaga agggatctgt cgcaagaaca
     3121 ggccagagct ctcgcagaac agaccgccgc ggcagagtca tttgcgagat ttgtaccagg
     3181 agaatttctg gaataccttg gaaaaaagaa catcatgagc atttcgcatg gcgacgcagt
     3241 tcgcaagcaa atggcagttc tgtttctgga catacgaggg ttcaccgaaa tgagcgaacg
     3301 gctgggctca gaaggaacgt tttcgtttct aaatgaattt cacggcggac tggaaccaat
     3361 cattcaaaag cacgacggat tcatcgacaa gttcatcggg gacgccatca tggcactctt
     3421 cccggcacca accggagcgg tccaggccgc catcgaaatg caaaactggg ttgcatacac
     3481 gggcaaagct agagttggaa ttggaattca tttcggagaa ttgatgcttg gaaccattgg
     3541 ctctcctcac aggctggaaa caactgtgat tggcgataca gtcaatctgg cttccagaat
     3601 agagagcatc aataagcagt acggtacaga aatcattgta tccgattcag tctataaact
     3661 cattcaggaa attccgcagc ttgctgcgcg cgaagtcgac gcgattcgtg tacgaggaaa
     3721 aaaacaaccc gttgtattgt atgaaatcat gaatggtctc tcacctgacg ttctggcttc
     3781 gcgcgaaaaa catatctctt cttatatggg agctttactc tcctatcgcg ccggtgattt
     3841 cgcggccgca aaaactcaat tcagtgaaca catgcagaac gtcaatggcg acagtgtggc
     3901 tgagatgtat ctcaaacgcc tcgatcgaat caatgaatca gcgccatggg atggaatctg
     3961 ggaagcggaa cattgatcaa cggcggagaa gactcaccac gggcctcgta cgcatcgaac
     4021 atctccgatc gaagccgtac gcgcgattgt gccggaagta ccgtctgaaa catcaaagat
     4081 gtaagcgttc gcagcattgc taatatcgtt ggtggaagac caataaactg tagcactcgg
     4141 attggcggta aagtaggtct gatttactgt tgggttcaca gactgcgtgt aatccatcaa
     4201 gcttgcgagc tcccgaacat tcggaagtct ccagtcagta aatccaccgt acacgagcga
     4261 ctcgcaaaat gtgagagcgt tcgtctgcgt gaagttagcc ggcgcgggac ccgcgcaatt
     4321 agctgaagtg tctgccgcgc caccggtctt gagagcgcaa accgtccagg tcaagcccgt
     4381 agtattgtca cgcacagaag gctcagttac accaagcctc acataatcct gcgtgggctg
     4441 ggcgggaccg gccacacaac gagcgaacgt gtctgttgcc agcgcattgg attgcgaagc
     4501 gaagaaacca aatttttgag caaacagata tgagtttgcg gggctggcca caacagttgt
     4561 agaagtccaa tgatacacgt tcgttccgga agctgtgttc ggaaagaaac tagaaggcat
     4621 ggctactgtt gcacctccaa agtaaggaag aagatgcatc tctcttacac tcggcaaacg
     4681 ccacacacga tcagcaatcg caactccgga acatgcggcg aatgcctgcg tacgagtcac
     4741 cgtagacaac gcagtgccag cgcaagaatt tccggtttgc ccaaaactac atttctgcca
     4801 gagaagatct gaaccgagca cctctcctac tgcatttccg agagttgttt cgtagagtat
     4861 gtaggcccct ggcgttgcgt attcggcgtc ctgccctgta cccgcgcagg agatcacagc
     4921 ccccgcggtc gtcgcacatg tggtctgtcc agtatcggga agagttcgcg gacgcgcaaa
     4981 gacaagggct gcgggccata tactacaaaa gacgctttcg caatgcgaag gatacttaca
     5041 ggacgcagag aatcccacga taaccaatag ttccatggct gcgaaccaac tgcgggcgaa
     5101 cgttgcagat gacgttgtca aaaatcgttt ccgcatgcga agatagaatt ttcgcagacc
     5161 gcttcaggtc aaatgtttaa cgccttacca ttgctctgcg atatgacgaa tcagcatgct
     5221 caagtcctga gtcagattcc atctccgccc atcaaagaca cgtgcatcac ttctttctgc
     5281 gtgacgattc cttctgcgaa cacagattca agcgtcgcac gatccatcat tcctgcgacg
     5341 gcgtttcgca cattcagcag aagacgacga aagcgacaca cgcgttcctg cttgcatggt
     5401 tcataacgag cgatggaaac gcaaccaatg ggcgcgagaa ttccgtcaaa ataacgaacg
     5461 atttctccca tcgtgatctg cgatggatgt tttgcgagtc gatatcctcc gtacttccct
     5521 gcagtgctcg acacccatcc ttgcgatttc atatccagca tgatgtgctc gagaaatctc
     5581 tttggtatgc gattggcttg cgctagatcg gaaatggaaa ccggttcccc ggtcgcatgc
     5641 tgctctacga gagtaaagat ggcacgcagc gcatagtctg tttttttaga aagcttcatg
     5701 aatctacgat cctttagatc gttatttgca ccactgccgc tcttcgtcaa gccaacgcta
     5761 agtgcgaaga acctggcgaa ccagctgcgc tgcatccgca atcagatatg gctttcgaat
     5821 gctggcgacg aatccgtatt ctgcgggggc cgtcattact ggatcttctg aatagccact
     5881 ggacgcaaac aatgcaattt ccgggtgatc cttcctgagt agtctggcaa gatcctttcc
     5941 acccatgtgt ccaggaaccg tcaaatccag aatcgcagca tgaaaaggtc tgccgctcct
     6001 ggccgcttca tcgcacaaaa tgagtgcttc ttcaccgtga gccgctacct gaacggaaaa
     6061 tcctgcagag gcaaagagat cctgagacac tgcacgaatg agttcttcgt catccataac
     6121 cagaagtctt tgttcacctg cgctgtgttt ggcgaaactt gctccgcgcg aattccctga
     6181 ctttgcaaca ggaatcaaaa tacgaaacgt tgctcctttg ccgggagtgg attctacatc
     6241 gatgaatcct gcatgtttct gaatgatgga aaagcaagtg gcaaggccca gccctgtacc
     6301 tcctgggcga gtgctataga atggatcaaa gatgtgcgaa atttgctccg ctggaattcc
     6361 gcgtccgctg tctgaaatct gaatctcgac gtatggccct gccgggtgaa cagaagtttc
     6421 attcagagaa cgattcgaag cgactgcccc tatgtttcct ccgtccggca ttgcctgacg
     6481 tgcattgatc aacaggttag aaaaaacctg gttcatctga ccggtatcga aattgcaagg
     6541 ccacaaatcg tctgctacgt ggcactgata gtgaacattg gatccggcca gaacaaattc
     6601 gcacgtttcc gaaaggagtg tcccgagatt gcccgattgc atgacaggat ctccgccgcg
     6661 tgcgaatgtg agaagctgtt gagcgagtcc tcgcgctctc gaaaacgcgg acagaccgcg
     6721 atcgagatat tcaataacgc ggctctctgt agtggcaccc cgcgcgagct ccatgtatcc
     6781 aaaaatagaa gaaaggagat tattaaaatc gtgggcgatt ccgcctgcaa gaacaccaag
     6841 tgaattgagg cgatccgcgc gctgggctgc ggccagcagg ttcattcttt cggtgatgtc
     6901 gtagagcatg accgcataca caccttcagg cgcatctcca ggagaaaccg cgctcacgat
     6961 tacaatccga tctgaaccat ctttgcagcg aatcgtcaat tccagggctt caaattgcgt
     7021 gccttctctg gtcgctttct ccattctctc ctgccaggac ttcatcaccc gttcgcgata
     7081 agccggctcc gggtaagcgc gaggccacca ttctgcgaga gtagggatgt ccgttaaatc
     7141 ataaccaaac gtgcgtgtaa aagcccggtt caaatatgtt atgttttgat tttcatcatt
     7201 cagagcgaag ggaataggag aaacatcaag tatctggcga aactgcgcat tcgtctctcc
     7261 catgggcgat cccgggcaat ttct
//
LOCUS       DAOQJL010000145         7260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5671_length_7260_cov_4.560999, whole genome shotgun sequence.
ACCESSION   DAOQJL010000145 DAOQJL010000000
VERSION     DAOQJL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5671_length_7260_cov_4.560999"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLG78_08135"
     CDS             complement(<1..485)
                     /locus_tag="PLG78_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23122.1"
                     /translation="MADRYAFLDLLKEQEALARGANLNDKVHHAIYKTIKSNSTVDGV
                     RHNTLYHLGVNVPEVLHLTKKDLVTVLTRLLQERLVTQIFVFLFEPGVPKPSFRPCFV
                     TIPPEEKQSVGQLYQEGIVQSSRALESYIDTLPPIGADAIWEDLEKDLEMEQVPQPSD
                     LP"
     gene            complement(487..1242)
                     /locus_tag="PLG78_08140"
     CDS             complement(487..1242)
                     /locus_tag="PLG78_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE23123.1"
                     /translation="MGRRPRKAFENREFLRSPAARKVRILTEVTYAEHVFAEQGVTDT
                     VVFFGSARVPAPEQKKEGKINEFGVYYDRARDLSREITSWSMAHAAPAKRNFVICTGG
                     GPGIMEAANRGALDAGGKSIGLNIELPEEQFPNPYITDELNFDFHYFFTRKFWFLYYA
                     RALVVFPGGFGTFDELFETLTLQQMKSIRNTIPVFLYGKDFWRRVIDFDFLAEIGMIA
                     RKDLELFHMVDSVEETMAHIVPALEESLRNPPE"
     gene            1257..2381
                     /locus_tag="PLG78_08145"
     CDS             1257..2381
                     /locus_tag="PLG78_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNE23124.1"
                     /translation="MKIGVDARPLAYAGNGNARYLFDMLRGAESGHDFVLYSHRPIHS
                     DYRSTLDRLGVSFRIDQSWTSRAGLLFLNFRLPGWLKEDLCDVYWGSLSMLPFFYKQR
                     VQIPSMVNFHDLNAFVAADTMTKANALQHGLTNGHTLRNADRVLCLSQTTANDIARFF
                     PAVKNKLEVVYPGIELKPIRPIAPEGTPGKLRHFYLMVGTIEPRKNQNTVIDAYLKLK
                     QELTSKHKLPPLVIVGRKGWGADVLFERLSSGILEKDGIYYIAGASDAVLEWCYKNSS
                     LYLFPSVHEGFGLPILEAAFRGIPLALSDIPIFREVGGKSAFAAPFDIQAWQEILRKP
                     PKKAPAFNKKVWSIENRAKKITQIVREIAEIRTTGRKQDS"
     gene            complement(2378..2608)
                     /locus_tag="PLG78_08150"
     CDS             complement(2378..2608)
                     /locus_tag="PLG78_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23125.1"
                     /translation="MLYLEREPGGRGPFRYNDEHDVIRRITVESCSGSCVCRLKYSDP
                     DLDEIFYDSAGCRKQSRYSNHPLYFRGSGCRI"
     gene            complement(2806..3930)
                     /locus_tag="PLG78_08155"
     CDS             complement(2806..3930)
                     /locus_tag="PLG78_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23126.1"
                     /translation="MRSKSNRLADFLKDKEQVRKFKESIRRIEFNAIVRPGSHSSSYR
                     KEYLEKTAGRLFPYPFLDHDFHIKCIADHVERAVSGEIVLHPVLAYASLFDLRSSYAP
                     ASKSKAKGKKKTSRAAARTRGKSGDALRKKRFLAVQKIMSRLRVPAYMKRAKTEFEIK
                     ESSVRAFEDMYGEFLNLRLRIQTLENEVLAYQNPDYAHPPIKAKLQEFQTQNNDLAMR
                     FVKEQNRADILESEIQRLTDHILAQPAEGELDSLKREYNVLSQKCDNLVSRNIELSNR
                     MKELGRSKQLEDILDGIRDKINSALRAGIHEAPDAILKSLSDEISQLKRARLYLGRAL
                     YDIGILYHRKGDRERAKEELRAARELGVRDAEVENILKQN"
     gene            complement(3986..>7260)
                     /gene="metH"
                     /locus_tag="PLG78_08160"
     CDS             complement(3986..>7260)
                     /gene="metH"
                     /locus_tag="PLG78_08160"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNE23127.1"
                     /translation="LNRSLSLSPDVNDPGFRAVTFDQVVKAYLEQIRGLVEGGSDILL
                     VETIFDTLNCKAAIYAIDQYVRESGNNIPVMLSGTIVDMSGRTLSGQTTEAFWLSISH
                     AKNLLSVGLNCALGSAQMRPFLTELSRVATVPVSLYPNAGLPNAFGGYDESPEYMAQQ
                     IDAYAGEGMINIIGGCCGTTPDHVKAMAQAALKHPPRKPAQTSGEFALAGLEPLTLRS
                     NMNFVNIGERTNVTGSPKFAKLILDGNYDEALSVARQQVENGAQIIDINMDEGMLDSE
                     AAMTKFLNLVAAEPDIAKVPVMVDSSKWSVLESGLKCLQGKSVVNSISLKEGEENFLN
                     ASRKIRSYGAATVVMAFDENGQADSYERRIEICKRAYDLLVKDGFPPEDIIFDPNILT
                     VGTGIAEHNNYAVDFIRATEWIKKNLPHARVSGGISNISFSFRGNNPVREAMHTAFLY
                     HAVRAGLDMGIVNAGMLGVYEDIPKDLLEMVEDVLLNRRPDATERLVEFADRFKGKAS
                     AEVKTDEWRNTSVEERLKHALVHGILDFIDQDTAEAHAKYGKPLSVIEGPLMGGMSVV
                     GDLFGAGKMFLPQVVKSARVMKKSVAYLIPFMEKEAAEGGGRKAAAKVLLATVKGDVH
                     DIGKNIVGVVLACNNFEVVDMGVMVPRDKILDQAREIGADVIGLSGLITPSLDEMVEV
                     AREMERRGLKVPLLIGGATTSKIHTAVKIAPSYRQPVIHVLDASRSVPVVNSLITPDQ
                     SAGFASSIEAEYEKLRHDFANREQSKKFLTLENARRNAPRIAHTGNPEPKRKSIQVLR
                     NYPLDELRQYIDWTPFFITWEMQGKYPAIFQDEKFGTEAKKLFDDANALLDQIIARKL
                     IHAHGVAMIWPANSVGDDIEVYADESRTSTIAVFHGLRQQTEKRAGEPNRCLSDYVAP
                     RGVKDYVGAFAVTAGDGVLDLAKSYEDKGDDYNSIMVKAIADRLAEAFAERLHEMVRK
                     EIWGYASDESLSAEDLVREAYQGIRPAPGYPSQPDHTEKQDLFRLLEATKHTGVSLTE
                     SLAMSPAASVCGIYLAEKQAEYFNLGKIEKDQVEDYARRKGMPVGEVEKWLSPALNYD
                     RP"
BASE COUNT         1729 a         1948 c         1714 g         1869 t
ORIGIN      
        1 ggaagatccg aaggctgcgg cacttgctcc atttccagat ccttctccag atcttcccag
       61 attgcatctg caccaatggg aggcagagta tcaatgtaag attccagggc gcgcgatgat
      121 tgaacaattc cctcctggta aagctggcct acggactgct tctcttccgg cggtatcgtg
      181 acaaaacaag ggcgaaacga tggctttgga actcctggct caaagagaaa tacgaagatc
      241 tgcgtgacca atcgttcttg cagaagacga gtcagaactg taaccagatc cttctttgtc
      301 agatgaagca cttctggaac gttcacaccc agatggtata gagtgttgtg cctcactccg
      361 tctactgtgg agttcgattt gattgttttg taaatggcat ggtgtacctt atcattgagg
      421 tttgcaccac gcgccagggc ctcctgttct ttcaggagat caagaaaggc gtaacgatcc
      481 gccatgctac tccggaggat tcctcagaga ttcttccagg gcaggtacaa tgtgcgccat
      541 cgtttcttct acagaatcca ccatgtgaaa taactcgaga tcctttctgg caatcatgcc
      601 aatctcagca agaaaatcaa aatctatgac tcggcgccag aaatctttgc cgtacaggaa
      661 tactggaatc gtatttcgta tactcttcat ttgctggaga gtcagggttt caaagagctc
      721 gtcaaatgtg ccaaacccgc ccggaaaaac aaccagggca cgggcatagt agagaaacca
      781 gaatttgcgg gtgaagaaat aatgaaaatc gaagttcaat tcatctgtga tgtatggatt
      841 tggaaattgc tcttcgggca actcaatgtt cagtccaatg ctctttccgc ctgcgtccag
      901 ggcgcctcta ttcgcagcct ccatgatgcc tggacctcca cccgtacaga tgacgaagtt
      961 cctctttgcc ggagcagcat gagccatgga ccaggatgtg atttcgcgcg aaaggtctcg
     1021 ggcgcggtcg taataaacac caaactcgtt gatcttccct tccttctttt gttcgggcgc
     1081 cgggactctg gcggatccaa aaaagaccac cgtgtccgta acaccctgct ctgcgaacac
     1141 gtgctcggcg taggtgacct ccgtcagaat ccgaaccttg cgggcagcgg gtgaacgtag
     1201 aaattcgcga ttctcaaatg ccttgcgcgg tcgcctgccc atgaaaagtt ctcctgatga
     1261 aaattggagt cgatgcccgg ccactggcct atgccgggaa tggcaacgcc cgctatctgt
     1321 ttgatatgct caggggcgcc gaatccgggc acgactttgt gctctattcg cacagaccca
     1381 tacactccga ttatcggtcc actctggacc gattgggagt cagtttccgc atcgaccaga
     1441 gctggacttc gcgagcgggg ctgctttttc tgaattttcg tcttcctggc tggctcaagg
     1501 aagatctgtg cgatgtatac tggggcagtc tgtccatgct tcccttcttc tataagcaga
     1561 gagtgcagat cccgtccatg gtcaatttcc acgacctcaa cgcattcgta gccgcagata
     1621 ccatgacaaa ggcgaacgca ctccagcacg gcctgaccaa cggacacact ctgagaaacg
     1681 cagacagggt gctctgcctg tcccagacca cggcaaacga catcgcacgt tttttccctg
     1741 cggtcaaaaa caaactcgaa gttgtttatc ccgggatcga actcaaacca atcaggccca
     1801 ttgcacccga aggcactccg ggaaagcttc gccatttcta tctcatggtg ggaaccattg
     1861 aaccgcgaaa gaaccagaac acagtcatag atgcatattt gaaactcaaa caggaactga
     1921 catcgaaaca caaactcccg cctctcgtga tcgtcggaag gaagggctgg ggcgcggatg
     1981 ttctctttga gagactttct tccgggattc tcgaaaaaga cggaatctac tacattgccg
     2041 gggcaagcga cgctgtactg gaatggtgct acaagaattc gtctctctat ctatttccaa
     2101 gcgttcatga aggatttgga cttccaatcc tcgaagctgc atttcgcggc atcccgcttg
     2161 ctctatccga catacctatc tttcgcgaag taggaggaaa gtccgcattc gccgcaccat
     2221 tcgatattca ggcgtggcaa gaaatccttc gaaaaccccc gaaaaaagcc cctgctttta
     2281 ataagaaggt ctggtcgatt gagaaccggg ccaaaaaaat cacgcagatc gtgcgcgaaa
     2341 tcgcagaaat caggactact ggcagaaagc aggactctta aattctgcag ccgctcccgc
     2401 gaaagtagag agggtgattc gagtatctgg actgcttacg acagccggca gaatcgtaga
     2461 agatctcgtc caggtccggg tctgagtatt tcaatctaca aacgcaggat ccactgcagc
     2521 tttctaccgt tatgcggcga atgacatcgt gctcatcatt gtacctgaaa ggacctcttc
     2581 ctccaggctc tctttcgaga tagagcatct caagggaaac gtacgtcatt ctatcgagga
     2641 aaatggctct gggagtatcc cctgcctcgc aggaaccctt gactgagttg aattcaatgt
     2701 cacaaatgaa cgattttcga tctgcagaga gagtgcagag cttgcctgga ggtgcagtct
     2761 tctcctgctt cgcgcaagcg aagatacaga agaaaagaac caggatcagt tttgctttaa
     2821 tatgttctcc acttctgcgt cacggactcc gagttctctt gctgcgcgca attcttcttt
     2881 ggctctttcc ctgtcccctt ttctgtggta gagaatacca atgtcataga gggcacggcc
     2941 tagatagagg cgagcgcgct tgagctgtga aatctcatcg ctcagactct taagaatcgc
     3001 atccggtgct tcatgaatgc ctgctctcag agcgctgttg attttgtctc tgatcccatc
     3061 gagaatatct tcgagttgct tggatcgtcc aagctctttc atgcgattgg acagctcaat
     3121 gttgcggctt acgaggttat cgcatttttg agaaagaacg ttgtattctc ttttcagcga
     3181 atccagttct ccttccgcgg gttgagccag tatatgatcc gtcagtctct ggatctcgct
     3241 ttctagaatg tccgcgcggt tttgttcttt tacaaaacgc atggccagat cattgttctg
     3301 agtctgaaat tcctggagct ttgctttgat gggcggatga gcgtagtctg gattttgata
     3361 ggccagcact tcgttttcta gagtttgaat ccgcagacga aggttcaaaa actctccgta
     3421 catgtcttcg aatgcccgaa cggaagattc tttaatttca aattctgttt tcgcacgctt
     3481 catgtaggca ggcacgcgaa gtctggacat gatcttctga actgccagaa agcgtttctt
     3541 acgaagagcg tccccggact tgcctctggt gcgagctgcc gcacgcgagg tctttttctt
     3601 gccttttgcc ttagacttac tcgcaggcgc ataagaactt ctcaggtcaa agaggctggc
     3661 atacgcaaga acaggatgca gaacgatttc tccgctgact gcacgctcta catgatctgc
     3721 gatgcattta atatggaagt catggtccag gaatggatag gggaagagtc tacctgctgt
     3781 cttttccagg tattcctttc tataagaaga cgaatggctc cccggacgca cgattgcgtt
     3841 gaactcaatc ctgcgtatgg attccttgaa cttccggacc tgctctttgt ccttcaggaa
     3901 gtctgccagt ctgttgctct tgctcctcaa aacccttgct ccgattagat tatcggaggc
     3961 tctcaaaggc acggctacct cttttttaag gtcgatcgta gttcagggcc ggggaaagcc
     4021 atttttctac ttcgcccaca ggcatgccct ttctgcgggc ataatcctca acctggtcct
     4081 tttctatttt gcccagattg aagtattctg cctgtttttc ggccagataa atgccgcaaa
     4141 cggacgcagc aggagacatg gccaggcttt ccgtcaaact gacgccagta tgttttgtag
     4201 cctcgaggag gcggaacaga tcctgcttct ccgtatgatc cggctgagac ggataaccag
     4261 gcgccggccg aatcccctga taggcttctc ggaccagatc ttccgcggac agagattcat
     4321 ccgaagcata gccccagatt tcctttctca ccatttcatg gagtctctct gcgaatgctt
     4381 ctgcaagccg atccgcaatt gcttttacca tgatggagtt ataatcatcc cctttgtctt
     4441 cgtatgattt tgcaagatcg agaacgccgt cccctgcagt cacggcgaat gcacctacat
     4501 aatccttcac gcctcgcgga gcgacataat cggagagaca acgattgggc tctcctgccc
     4561 tcttttcagt ctgctggcgc aggccatgaa aaaccgcaat cgtagaagtg cgcgattcat
     4621 ccgcatacac ttctatatca tctcccaccg agttggcagg ccagatcatc gcaactccgt
     4681 gcgcgtgaat gagctttcgc gcaatgatct gatcgagcaa tgcgttggcg tcatcaaaaa
     4741 gcttctttgc ctcggtgcca aacttctcat cctgaaagat cgcaggatat ttcccctgca
     4801 tttcccaggt aatgaagaaa ggagtccagt caatgtattg cctgagttca tcgagtggat
     4861 agtttctgag aacctgaatg ctctttcttt tgggttcagg atttccagta tgcgcaattc
     4921 tgggcgcatt cctgcgagca ttctccagcg tgagaaattt cttactctgt tcgcgattgg
     4981 caaagtcatg gcggagcttt tcgtactcgg cttcgatgga agatgcaaaa cccgcactct
     5041 ggtcaggagt gatgagactg tttacaactg gcacactacg cgatgcatcg agcacgtgaa
     5101 tgactggctg cctgtaggaa ggagcaatct tgactgcagt gtgaatcttg gaagtagtgg
     5161 cgccgccaat caagagcgga actttgagtc cgcgtctttc catttcgcgc gctacttcca
     5221 ccatctcgtc caatgacgga gtgatcaacc cggacagacc aattacatcc gcgccgattt
     5281 cgcgagcctg atccagaatc ttgtcccggg gaaccatcac acccatgtcg acgacttcga
     5341 agttgttgca cgcaagtacg actccaacga tgtttttgcc aatatcgtgg acatcacctt
     5401 tgactgttgc aagaagtact ttggccgcag ctttcctgcc tccgccttcc gcggcttctt
     5461 tctccatgaa aggaatcagg taggccacag attttttcat cactctggca ctcttcacca
     5521 cttgcggcaa aaacattttg cccgcaccaa acaggtctcc cactacagac attccaccca
     5581 tgagaggccc ttctatcaca gacagtggtt tgccgtactt cgcatgcgcc tccgccgtat
     5641 cctggtcgat gaaatccagg atgccatgca ccagtgcatg tttgagtctt tcttctacag
     5701 atgtatttct ccattcatcc gtcttcacct cagcggacgc tttgcccttg aacctgtctg
     5761 cgaattcaac caggcgttct gttgcatccg gcctgcgatt taaaagaaca tcctctacca
     5821 tctccagtag atcttttggg atatcctcgt aaactccaag catccccgcg ttcacgatcc
     5881 ccatgtcgag ccccgcgcgc actgcatgat aaagaaacgc agtgtgcatt gcttcccgca
     5941 ctggattgtt gccgcgaaaa gagaaagaaa tattgctaat acctccgctc actcgcgcat
     6001 gcggaagatt cttcttgatc cactcggtcg cgcgaatgaa atccacagcg tagttgttgt
     6061 gttctgcgat cccggttccc acagtcagaa tgttcggatc gaaaataatg tcctctggcg
     6121 gaaatccgtc ttttacaagc aaatcatagg ctcttttaca gatttcaatc cggcgctcgt
     6181 atgaatctgc ctgaccgttc tcgtcaaacg ccattactac agtagccgca ccataggagc
     6241 gaatcttgcg cgatgcgttc aggaaattct cttcgccttc tttgagcgaa atagaattca
     6301 caacactttt tccctgcagg cacttcaagc cagactcaag tacactccat ttggaagagt
     6361 ccaccatgac cggaactttc gcaatgtccg gctctgccgc gaccagattc aaaaactttg
     6421 tcatggccgc ttctgaatcg agcatgcctt catccatgtt gatgtctatg atctgcgctc
     6481 cgttctccac ctgctggcgg gcaacgctga gtgcttcgtc gtagtttcca tccaggatca
     6541 gcttcgcgaa ctttggagac cctgtgacgt ttgtacgttc gccaatgttt acaaagttca
     6601 tgttgctgcg aagggtaaga ggctcaagac cggccagcgc aaactcaccg ctcgtttgcg
     6661 caggcttgcg cggaggatgc ttgagcgccg cttgcgccat cgccttgaca tgatcgggtg
     6721 tagtcccgca gcaaccgccg atgatgttga tcatcccttc cccggcatac gcatcaatct
     6781 gctgtgccat atattctgga ctttcatcat acccgccgaa tgcattggga agtcccgcgt
     6841 ttggataaag acttaccgga accgttgcca cgcgcgaaag ttccgtgaga aacggcctca
     6901 tctgcgcaga accaagagca cagttgagcc ccactgaaag cagatttttc gcatgtgaaa
     6961 tagaaagcca gaatgcttcc gtagtctgcc cggagagagt tctccctgac atatccacaa
     7021 tggttccaga aagcatgacc ggaatattgt taccgctttc tcgcacatac tgatcgatcg
     7081 catagatcgc agctttgcag ttcaaagtat caaaaatagt ttctacaagt agaatgtcag
     7141 agccgccttc tacgagacct cggatttgct ccagatatgc ctttactacc tgatcaaaag
     7201 taaccgcgcg aaatccagga tcattgacgt caggagaaag agaaagactt ctgttgagcg
//
LOCUS       DAOQJL010000146         7249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5685_length_7249_cov_4.407562, whole genome shotgun sequence.
ACCESSION   DAOQJL010000146 DAOQJL010000000
VERSION     DAOQJL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_7249_cov_4.407562"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLG78_08165"
     CDS             complement(<1..828)
                     /locus_tag="PLG78_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE23128.1"
                     /translation="MKSFEIQSAFGIENLKISERPDPVPGHGEVLVRMRAASLNYRDF
                     LTVIGQYNPKYKLPLIPGSDAAGEVVSTGPGVSEYKTGDRVMSCFALDWISGTPGIKQ
                     VRNSSLGGPLDGTLTELRVFPEKSLVRIPDFMTYEEASTLPCAGVTAWSALVTEAVVP
                     PGSTIVVLGTGGVSIFALQIGKMLGARVIVTSSSNEKLELARKLGADEVINYREKPQW
                     SKEVRKLTSMEGADHIIEVGGAGTLEESLRAVKFFGQISLIGILAGSARDLNLLPILM
                     "
     gene            complement(887..2131)
                     /gene="ccrA"
                     /locus_tag="PLG78_08170"
     CDS             complement(887..2131)
                     /gene="ccrA"
                     /locus_tag="PLG78_08170"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNE23129.1"
                     /translation="MAQEIVPVGTLPPLGEVPKKMHAQLVRPERYGEPRTAFQVEQID
                     VPDIAPDEVLVAVMAAGVNYNNVWAALGYPVDVIAARNKKGEPEKFHIGGSDASGIVF
                     KVGSDVKNVKVGDEVVVHCGIWDRNDPWVKAGKDPMFAPSQIIWGYESNWGSFAQFCK
                     VQDHQCLPKPPHLTWEEAAAYMLVAATAYRMLHHWSPNSVQKDDVVLIWGGAGGLGSM
                     AIQIAKAAGALPIAVISSDDKIEYCKKLGAAGTINRNNFKHWGALTTEINKGEVFGQW
                     TKSAREFGKAIWDIAGKGRNPQIVFEHPGESTLPTSVFVCETGGMVVICAGTTGFNAT
                     GDLRYLWMRQKRLQGSHFANDENCKGLNDLVMQKKVDPCLSKTYKFEETGACHQLMRE
                     NKHPHGNMSILVGAPSEGLGRK"
     gene            complement(2154..2333)
                     /locus_tag="PLG78_08175"
     CDS             complement(2154..2333)
                     /locus_tag="PLG78_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23130.1"
                     /translation="MRIVLLVVTLAIVLGLQVGLTKLFAPYTHMEMEFRFIRYFLVCI
                     VGFLLIPWSAKKLLS"
     gene            complement(2366..3064)
                     /locus_tag="PLG78_08180"
     CDS             complement(2366..3064)
                     /locus_tag="PLG78_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE23131.1"
                     /translation="MFESLEQTLVHVNIAAQKIPGLAGAMRAASFLGVETFYFALVPA
                     LFWLFSRRAALELLLMIIATAYTNSILKWVFSRPRPYWISDSVQALAEETTYGIPSGH
                     AQNAVVVWFMLAVFAVRKWPNRKTAIYGVATFIVALISFARIYLGVHFIEDIVAGWLF
                     GSILLALYFKFHKPMEEWWRVSPARVGVVLLPVVLLWFAFKVRTLSPGIEDLFPWRPS
                     SAPRAVSWVSSLSF"
     gene            3141..3986
                     /locus_tag="PLG78_08185"
     CDS             3141..3986
                     /locus_tag="PLG78_08185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE23132.1"
                     /translation="METQIVGFPLDETERRIRHVISIMPALGRSLLSSQDSLNIRNSS
                     SSQSDKILMEKADRTISHTIVRSLQTQFPEDKIVSEDEGQVGSDGDFEWWCDPVDGTR
                     NFIHGIPFFCISMGLTWRGSPVGGVVHVPAMGQTGLTYHAIVGQGAFKNQNPIHVSTV
                     SQLERAVMASGLPYRRKEIIGDILGDIFAFVTTGIGLRRTGSVILDLCWIAEGKFDAL
                     WDRDISPWDSCAASVILFEAGGKISGYLGEKFDIQQTRIVASNGILHEEAVSVLQKAR
                     KTTMN"
     gene            complement(4005..5222)
                     /locus_tag="PLG78_08190"
     CDS             complement(4005..5222)
                     /locus_tag="PLG78_08190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE23133.1"
                     /translation="MHRAVQITTWQRLTRPSSNEVPLLDGLRALSLVAVLLFHYWASL
                     RHFARRVPDSVDVFLLNLNTGVVLFFVLSGFLISRGLYNEHRRTGEISFRSFYVKRTF
                     RILPAYYFFLLFALLFIVINRAKVVHSTGILSPTADWYFDFLFISNYLPGIQGHTWTL
                     SIEEQFYLLFPLLAAFLFKLRRDRLLLALVGIYLIPLAFRILSMVTLPEGEYAPHVDR
                     ASLTRFDDIIVGILLAVGGKELFPRKFTALIGLAGLTLTAIVLLLPAQVPDWFVVVRP
                     NFTNLGFGAIVYWAIHREDVKVPKLVTAVARLSYSMYLWHMIAGAFGAGSVMHLIYDG
                     TKIGAGPLLRGLLVSALATGLCGYLSYRFVEQPFLRLREKLETPPEDRKPDDPSTDDQ
                     DSQTPGELVVTPF"
     gene            complement(5529..6614)
                     /locus_tag="PLG78_08195"
     CDS             complement(5529..6614)
                     /locus_tag="PLG78_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE23134.1"
                     /translation="MRRQVDSLSAGPKKTPGWIAATVALLFFVSCSMFQQNESSDKTN
                     SAVAFLLAGYLLNTQSAVPTVQRNSTYNVVKTTHVYAQALSHSNWGSTTTSVVDLSLD
                     LYRPENAPGNRPAMILIHGGGFMTGAKDDANMVDMANYFASRGWVCISIDYRLAAARG
                     TLSTAWGNYVLANVVSTEREQSYAMYPAARDAKAAVRWLYANAGTYGINTNYVTALGG
                     SAGSFLANMLGITNASDFRDESLSIVDTTLSTTNLSSGSTVHTVIDHWGGIHMMTYLQ
                     AIDGQSRFDAKDPPISIVHGTADAEVPFSQAQALRDAYISTGAPYEFNALVGAGHGAW
                     SATINGRSLSENAFLFITQKQNLKVLQ"
     gene            complement(6751..>7249)
                     /locus_tag="PLG78_08200"
     CDS             complement(6751..>7249)
                     /locus_tag="PLG78_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE23135.1"
                     /translation="FLSVWRENRSELVSFIQKLPEDQFAKAPASGGWSASEIAEHLYL
                     TQWNLARSLPIVLGGKFGKDKAELKSDLRYETLSKLLIRPRGAKNPIEVGPKGGMDKA
                     ATLESLQKSMDKLDKAVSSKTKDQLQSRGMDHPFFGAISMFDWLWVMTNHEATHIQTL
                     ISKYS"
BASE COUNT         1919 a         1810 c         1795 g         1725 t
ORIGIN      
        1 catcaagatg ggaagtagat ttagatcgcg agcagatcca gctagaattc cgatcaagct
       61 gatctgtcca aaaaacttca ccgcgcgaag cgattcttcc agagtcccgg ccccgcccac
      121 ttcaatgatg tggtccgcgc cttccatgct ggtgagcttg cgcacttctt tggaccattg
      181 cggcttttcg cggtaattga tgacttcatc tgcccccagt tttcgcgcaa gctccagctt
      241 ctcattcgag ctcgaagtaa caatgactct cgcgccgagc attttgccga tctgtaaagc
      301 gaagatcgaa acgccgccag tacccagaac cacaatcgtg ctgccaggtg gaactacagc
      361 ttctgtgacc agagcgctcc atgcggttac acccgcacac ggaagagtgg atgcttcttc
      421 gtaggtcatg aaatcgggaa ttcgcaccag gctcttttca ggaaaaactc taagttctgt
      481 gagtgttccg tccagtggac cgcccagaga agaattgcga acttgtttga tacctggagt
      541 gccggaaatc cagtccagag cgaaacagga catcactctg tctcctgtct tgtattcaga
      601 aactccagga ccggtggaga ccacttcacc cgcagcatct gaaccaggga tcaaagggag
      661 tttgtatttt ggattgtact gaccaatgac tgtgagaaaa tccctgtagt tcagggaagc
      721 ggcgcgcata cgaacgagca cttctccgtg acctggaacc ggatccggtc tttccgaaat
      781 cttcagattt tctattccaa acgcggactg aatttcaaaa gacttcattg cgattctcct
      841 gataggcgat ccatcggcgc ccgcgactgc ggacgccgga atctatttac ttgcgaccca
      901 ggccttcgga aggagcgcct actagaatcg acatgttgcc atgaggatgc ttgttttcgc
      961 gcatcaactg gtggcatgca cctgtttctt caaacttgta agttttggag aggcatggat
     1021 ccactttctt ctgcatgacc aggtcattga gtcctttgca gttttcgtcg tttgcgaagt
     1081 gagaaccctg caatctcttc tgacgcatcc agagataacg aagatcccct gttgcattga
     1141 agccagttgt tccagcgcag atgactacca taccgcctgt ttcgcatacg aatacagaag
     1201 ttggtagagt ggattctcct ggatgttcaa atacgatctg aggattcctg ccctttcctg
     1261 cgatgtccca gattgctttt ccaaattcgc gcgctgattt ggtccactgg ccgaacactt
     1321 cgcctttgtt gatttcggtt gtcagagcgc cccagtgctt gaaattgttt ctgttgattg
     1381 tgccggctgc acccagtttc ttacagtatt caatcttgtc gtctgaactg atcactgcaa
     1441 tgggaagagc ccctgctgct ttcgcaatct gaatggccat ggaaccaagt ccacctgcac
     1501 cgccccagat caaaaccaca tcgtcttttt gaacgctgtt gggagaccag tggtgaagca
     1561 tgcgatacgc agtggcagca accagcatgt atgctgcggc ttcttcccag gtaagatgag
     1621 gaggttttgg aagacattga tgatcctgca ctttgcagaa ttgagcgaag gatccccagt
     1681 tgctttcata accccagatg atttgcgatg gagcaaacat tggatctttt cctgctttca
     1741 cccacggatc gttgcgatcc cagattccgc aatggacgac cacttcatcc cccactttta
     1801 cattcttcac atcgcttccg actttaaaaa cgattccaga agcatctgaa ccgccgatgt
     1861 ggaacttctc aggttctcct ttcttgtttc gcgcggcaat tacatctact ggataaccca
     1921 gagcggccca aacgttgttg tagttcacac ctgctgccat cactgcaacg agcacttcat
     1981 ccggagcaat gtccggaaca tcgatctgct ctacctgaaa cgcagtgcga ggttctccgt
     2041 atcgctctgg tcttacgagc tgagcgtgca tttttttggg aacttctcca agcggaggga
     2101 gtgttccaac gggtacgatt tcttgtgcca ttcgcccaaa agtaccggat cactcaggag
     2161 agcaattttt ttgcagacca gggaatcaga agaaaaccaa caatgcaaac gaggaaatac
     2221 cgaatgaatc tgaattccat ttccatgtgg gtgtaaggcg caaataactt cgtcagcccc
     2281 acctgcaaac ccaggacaat cgccagagta accacaagaa gcacaattcg catgcgcaaa
     2341 tgaggttgga aactcaccac aggaatcaaa aagacaatga tgagacccag gagactgccc
     2401 tgggtgctga tgacggtcgc cagggaaaga ggtcctctat accgggactg agtgtacgca
     2461 ccttaaaagc aaaccacagt aagacgactg gcaaaaggac gacacccact cgagcaggag
     2521 agactcgcca ccattcttcc atcggtttat gaaatttgaa ataaagagcc aggagaatgg
     2581 agccaaacag ccagcctgca acaatgtcct ctataaaatg caccccgaga taaattcggg
     2641 cgaaactgat caacgcgacg atgaaggttg cgactccgta gatggccgtt ttacgattcg
     2701 gccattttcg cacggcaaac acagccagca tgaaccagac aaccacagca ttctgagcat
     2761 gtccggacgg aattccatat gttgtttcct ctgcaagagc ctggacgctg tcagagatcc
     2821 agtagggcct ggggcgagag aacacccact tgagaatgct attcgtgtaa gcagtagcga
     2881 tgatcatcag gagcagttcc agagcggcgc ggcggctgaa gagccagaac agagccggca
     2941 caagtgcaaa ataaaacgtc tctacgccca gaaagctcgc agcccgcatg gcgcccgcaa
     3001 gcccggggat cttctgtgcg gcgatgttta cgtgcaccag agtctgctct aaggattcaa
     3061 acatacggca actcaccatg ggaatctaga ttgaacaaga aaaacttgcc cgcgatccgg
     3121 ggatcagtcg catggaataa ttggaaacac agattgtagg attcccgctc gatgaaacgg
     3181 agcgacgcat tcggcacgta atttcgatca tgcccgcact cgggcggagc ctgctctctt
     3241 cgcaggactc attgaacatt cggaattctt cgtcctcgca gtcagacaaa attctgatgg
     3301 aaaaagcaga tcgcacgatc agccacacca ttgttcgctc tctgcaaacg cagtttccgg
     3361 aagacaaaat cgtatctgaa gacgaaggac aggtcggatc ggatggcgat ttcgaatggt
     3421 ggtgcgaccc tgtagacggc actcgcaatt tcattcatgg tattccattc ttctgcatat
     3481 ccatgggact gacgtggaga ggaagtcctg tgggcggagt cgttcatgtg cctgccatgg
     3541 gacagaccgg gctcacgtat catgcgattg taggacaggg agcatttaaa aatcaaaatc
     3601 caattcacgt ttctactgta agtcagctag aaagagcggt gatggcttcc ggcctgccct
     3661 accgcagaaa ggaaatcatt ggagatatac tcggtgacat tttcgcattc gtgaccacag
     3721 gaatcggcct tcgcagaaca ggctctgtga ttctggatct ttgctggatt gcggaaggta
     3781 aattcgacgc gctctgggac agagacatca gtccctggga tagttgtgct gccagcgtca
     3841 ttttatttga agcgggcgga aagatttccg gctatctggg agagaagttc gacatacaac
     3901 agactcgcat tgttgcgtcg aatggaattc tgcacgaaga agcagtcagc gttttacaaa
     3961 aagcgcgcaa gaccacaatg aactaaccgc gcgaaagtcg tgcatcagaa gggcgtgacc
     4021 accagttcac caggtgtctg cgaatcctgg tcatctgtgc tcgggtcatc gggcttcctg
     4081 tcttctggag gagtttccaa tttctcccga agacggagaa agggttgttc gacaaatcga
     4141 taagacagat aaccgcaaag tcccgttgca agcgctgaaa cgagcaatcc tcgcaacaaa
     4201 ggtcccgcgc caattttcgt tccgtcgtaa atgaggtgca tgacagaccc cgctccaaat
     4261 gcaccggcaa tcatgtgcca cagatacatg gaatagctga gtctggcaac tgccgttacg
     4321 agttttggaa ctttcacatc ttcacgatga atagcccagt agacgatcgc cccgaagcca
     4381 agattcgtaa agtttggcct gacaaccaca aaccagtccg ggacttgcgc gggcaacaga
     4441 agcacaatgg cggttaacgt caagccggcg aggccaatga gtgcggtgaa ctttctggga
     4501 aagagttctt ttccccctac tgccagcaga attcccacaa tgatatcatc aaaacgagtc
     4561 aggctggccc tgtccacatg aggagcatat tcgccttccg gtagagtaac catggagaga
     4621 atgcgaaacg caagtggaat cagatagatt ccaaccagtg ccaggagtaa gcgatctcta
     4681 cgaagcttaa acagaaaagc agccaggaga gggaagagaa ggtagaactg ctcttctata
     4741 gaaagagtcc acgtatgccc ctggatgccc ggtaaataat tggaaatgaa aaggaaatca
     4801 aaataccaat cagcggttgg tgataggatc cccgtgctgt gaaccacttt tgctctattg
     4861 atcacaatga acaacagcgc aaatagtaag aagaaatagt atgccggcaa aattcgaaac
     4921 gttctcttca cataaaaaga gcgaaaagaa atctccccgg tgcgccggtg ttcattgtag
     4981 agtcctctgg aaatcagaaa accgctgaga acaaaaaaga gaacaacgcc tgtgtttaga
     5041 ttgagcagaa acacatctac agaatctgga acgcgcctgg caaaatgacg aagcgaggcc
     5101 cagtagtgaa aaagtagaac tgctacgagt gacaaagcgc gaagtccgtc tagcaaagga
     5161 acttcgttgc tcgatggtct ggtcagccgt tgccaggttg tgatctgaac cgctcgatgc
     5221 atatgaaaca caacaactca ggtcatatct tttgcgcttt tatgttgagc cggatgcatt
     5281 tgtgtctaat tcgtcaccag gatgtaacga catgctagtc gcgcacgttt cacaaccgtt
     5341 cgcgccactg gaaaaacaaa acagcgcgtt cctcgaccag acctttgcag acaatgtgcg
     5401 cacacattgt gttgtttatg tccgatttcg tatctttcat gtaacgcttg gttagttgct
     5461 ccagcagatg gcatggtgta tggatggttc catggttatg gcgacctgag agagtgagcg
     5521 ggaagccatt actgaagcac tttcaaattt tgcttctgag taatgaagag aaaagcattt
     5581 tcagaaagag atctgccgtt gatcgtcgca gaccaggcac cgtgcccggc tccaaccagt
     5641 gcgttgaatt cgtacggcgc acctgtggaa atgtatgcgt ctctgagagc ctgggcctgg
     5701 gaaaagggta cctctgcgtc tgctgtacca tgaacaatgc taatgggcgg gtcttttgca
     5761 tcaaatctgg actgaccatc gattgcctgc agatacgtca tcatgtggat gccaccccag
     5821 tgatcgatca ctgtatgaac ggtagatcca gacgaaagat tggtagtgga aagagtagta
     5881 tctacgatac tcaagctttc atcgcgaaaa tcgctcgcat ttgtaattcc aagcatgttg
     5941 gcgagaaacg atcctgcgga tccaccgagg gctgttacat agtttgtatt gattccataa
     6001 gtcccggcat tcgcatacag ccagcgaacg gcagccttcg catctctggc agcaggatac
     6061 attgcatagc tttgttcccg ctccgtagaa acaacattgg cgagtacgta attcccccac
     6121 gccgtagaaa gagtcccgcg cgccgcagcc agacgatagt caatggaaat gcaaacccag
     6181 ccgcgacttg caaaatagtt ggccatatct accatgttcg catcgtcctt tgcgcctgtc
     6241 atgaatccgc cgccatgaat gaggatcatc gcaggcctat ttccaggtgc attctcaggg
     6301 cgatacagat ccagacttaa gtctaccaca gaagtggtgg tagatcccca gttggagtga
     6361 ctgagcgcct gagcgtatac gtgagttgtc ttcacaacgt tataggtgga gtttcgctgc
     6421 acagttggaa cggcgctttg tgtattgaga agatatccgg ccaggagaaa agccacagcc
     6481 gaattcgttt tgtcgcttga ttcgttttgc tgaaacatcg agcaagaaac gaagaacaaa
     6541 agcgcaaccg tagccgcgat ccatccgggt gttttttttg gccctgcaga aagagagtcc
     6601 acttgccttc tcatatctaa taaacgaaaa gacagaactt gtttgcaact attcttgcgc
     6661 gcgcacggag atgaaccctg gacaaatgga gaaaaagaac gaatgtccag ggtttttcag
     6721 aaggaatttt gcgaatctaa gtacaaatac ttaggaatat ttactaatta aagtctgaat
     6781 gtgagtggct tcgtgattgg tcatcaccca caaccagtca aacatgctaa tggcgccaaa
     6841 gaaaggatga tccatcccac gcgactgaag ttgatctttt gttttggaag aaacagcttt
     6901 gtccagcttg tccatggatt tttgaagcga ctcaagtgtg gctgctttgt ccattccacc
     6961 ctttggtcct acctcgattg gattttttgc accgcgcggc ctgatcagaa gtttgcttag
     7021 agtctcgtat ctgagatccg atttgagttc cgctttgtct tttccgaatt ttccgccgag
     7081 tacaatcggc aaggatctgg ccaggttcca ctgagtgaga taaaggtgct ctgcgatttc
     7141 agacgcgctc cagcctccgg aggcaggtgc ctttgcgaat tgatcctctg gaagtttctg
     7201 gatgaaactt acaagctctg atctattctc tcgccacaca gagaggaaa
//
LOCUS       DAOQJL010000147         7248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5689_length_7248_cov_3.850549, whole genome shotgun sequence.
ACCESSION   DAOQJL010000147 DAOQJL010000000
VERSION     DAOQJL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5689_length_7248_cov_3.850549"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLG78_08205"
     CDS             <1..1186
                     /locus_tag="PLG78_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE23136.1"
                     /translation="DTIQALKELDRNSSRFLIEQGIIGQVRRSPLGVVLCMGPYNYPL
                     NETFATLIPALVMGNTCVFKPPKLGILLYRPLLEAFRDSLPKGVINTVYGSGEEIVPP
                     IMESGKIDVIAFIGSARVADTLKKAHPHPHKLRSVLGLGAKNPAIVLKDADLDTTIPE
                     AVTGALSYNGQRCTALKIFFVHEDIAEVFATRLSEKIAQLTFGMPWSAGTKLTPLPVP
                     GKSEYLDGLVKDAQSKGARVMNANGGAFYRTFFYPAVLFPVNDSMRAYHEEQFGPVIP
                     IVPYSNLDDAMNYVYNSHYGQQLSIFGSDPAVIAKMVDPLVNQVCRVNLNSQCQRGPD
                     TFPFTGRKGSAEGTLSVSDALRSFSIRSLFAAKESAQNKAIIETILRDDTSSFISTDF
                     LF"
     gene            1128..2051
                     /locus_tag="PLG78_08210"
     CDS             1128..2051
                     /locus_tag="PLG78_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE23137.1"
                     /translation="MKRFCETIRPVSFRLIFFFEHGYSRSSAGPCSPAHRRKICASRS
                     SCSWRKGSRAFSFSGSHGSMGSFARRNERCGPHPHIGLATVTYLFEGSILHRDSVGSV
                     QEIHPGDVNWMNAGKGIVHSERRPPSLANRAYRIHGLQAWVGLPLSQELSEPFFHHHP
                     RRDLPHFTIDGVEISLLAGSALGVTAPAQTFSDLFYMDLRIPRGGSWTFVQDHRECAL
                     YIVEGTLRIHGQNFVSHSLVVFEPGKEVKMDAQDGVIAVLLGGAPLDGPRHMWWNFVS
                     SSRDRIEEAKRSWASGTFGEIEGETGSIPLP"
     gene            2102..2413
                     /locus_tag="PLG78_08215"
     CDS             2102..2413
                     /locus_tag="PLG78_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23138.1"
                     /translation="MLTSPEKKATDKPEMFDTLGIYRRLKRARASEPVAREIAMAMGE
                     IVQSQLATKEDLLVVRRDFQDVKQELKLEISASRMETLRWMMGMMVAQLALLVSILKI
                     L"
     gene            complement(2466..2849)
                     /locus_tag="PLG78_08220"
     CDS             complement(2466..2849)
                     /locus_tag="PLG78_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23139.1"
                     /translation="MRKLFAITPFLLLAALACSKPSSSSGGAEDYKAESDKIKSQMCG
                     KMEECMQEMMKGMPENMRQMMSAKFSKESCMAQASAVPQKDKPENFTKEDMEAAKACG
                     EAMAKASCTDIKNHNVAGCEKFMNK"
     gene            2989..3873
                     /gene="cysM"
                     /locus_tag="PLG78_08225"
     CDS             2989..3873
                     /gene="cysM"
                     /locus_tag="PLG78_08225"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF008735.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNE23140.1"
                     /translation="MDKAFRLSQLIGNTPLVDLSRKGSRVRLFGKLKGNNPAGSVKDR
                     PALWMMEQAELRGELKPGMRIVEATSGNTGIALAMVAAVKGYGIELIMPEDATRERVL
                     AMQAYGATVTLTDASRSMEGAIDLAHERFAEGGFWMPNQFANPDNWKSHYHTTGPEIW
                     RDTEGRVTHFVSSMGTTGTIMGTSRFLKEKNPAIQIIGVQPEDGAKIPGIRRWPVEYL
                     PAIFEPARVDRVMDVTQQDAEATTRLLAKRDGVFCGMSSGGAICAASRLAEQLEDGVI
                     VTILCDRGDRYLSSDLFG"
     gene            complement(3878..5056)
                     /locus_tag="PLG78_08230"
     CDS             complement(3878..5056)
                     /locus_tag="PLG78_08230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNE23141.1"
                     /translation="MKDPAYILEGRRLPVGAFLGSLSRLGPVELGVLAAQAAIEAASV
                     DHGAVSSIICGNVVPSGPESLYLARHVGLKAGLPISASALNVNRLCGSGLEAIAQAAH
                     QIRAGEDLSLACGVESMSQSPYLAAGARTGIRYGDGKLIDLLSSALTDPYIGQAMGRT
                     AETLAQEYGISRQDQDAWALVSQTRAEAAQKSGKLSDEIKSVQVKDGKEMKVFDRDEF
                     VRGTAAGAKLAGLAPAFLENGTVTAGNASGINDGASAVVVCSERYLQSSGKKPLAKIL
                     GWASRGCPPEKMGIGPVHAIPAALASAGIQQEQIQLWEINEAFAAQYLAVAKSLKLDP
                     EITNVNGGAIAIGHPLGASGNRILLGLAKELRRRKLKYGCASLCIGGGQGIAMVIESV
                     "
     gene            5390..6001
                     /locus_tag="PLG78_08235"
                     /pseudo
     CDS             5390..6001
                     /locus_tag="PLG78_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
     gene            6028..7116
                     /locus_tag="PLG78_08240"
     CDS             6028..7116
                     /locus_tag="PLG78_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNE23142.1"
                     /translation="MELIKKIFDRRTATIAAVSSIAAVALALGGGIVSADVKTAVGSD
                     VSSNELRSITVESWDKDYSNGGYGWEVSTDKDMTSKDGQYQPNAQSLQAEREVKLIKG
                     TPQDIRENVNYPEARVLGVKFGFTFPGYNVVSIRPPHVDQYVVERPRPFLNEYAVTGD
                     YKSRSCYKNPALSSVNAGRAMVVDCLYGIELPGEVHKVSVWVMGRGNDYDLEGWFEDW
                     KGATHILKFGSVNFIGWRPMSIEIPKSIPQDVNSFPAIKTLVFKQFKLRSKPHTSLES
                     VYIFFDELRVLTDIFEVHFDGAALDFDKADCDRKNHLLKMIRDNARNPDAYTALVDCS
                     KAPGPAAPIQSGNTQPSQPAQPAQPAQP"
BASE COUNT         1738 a         1804 c         1925 g         1781 t
ORIGIN      
        1 cgatacaatt caggctctca aagaactcga tcgaaattcg tcgaggtttc taatagaaca
       61 gggaatcatc gggcaggtac ggcgttcacc actcggagtc gtgctctgta tgggcccgta
      121 taactacccg ttaaacgaaa ctttcgcgac tctgatcccg gcactcgtga tgggaaatac
      181 atgcgtattc aaaccaccca agcttggaat tctgttgtat cgtcctcttc ttgaggcatt
      241 tcgagattct ctgccaaaag gtgtaatcaa tacagtgtac gggtcggggg aggaaattgt
      301 gccccctatt atggaaagcg gcaagataga cgtgattgcg ttcattggtt ctgctcgcgt
      361 cgcagacact ttgaagaagg cgcatccaca tcctcacaaa cttcgttccg tgcttggcct
      421 gggtgcaaag aatccggcca tcgtcttgaa agatgcggac ctggacacga ccattcccga
      481 ggcagttacc ggagcattgt cgtataacgg tcagagatgt acggcactca aaatcttttt
      541 tgttcatgag gacattgccg aggtttttgc aaccaggctt tcagagaaga ttgctcaatt
      601 gacttttgga atgccgtggt ccgccggaac gaagctcact cctcttcccg tccccgggaa
      661 aagtgaatac ctggatggac ttgtaaaaga tgcgcagagc aagggcgcac gagtgatgaa
      721 tgcgaatggt ggagcgtttt ataggacgtt cttttatcct gcagttcttt ttcccgtgaa
      781 cgattccatg cgcgcttacc atgaggagca gtttggtccg gtcattccta ttgttccata
      841 ttcaaacctg gacgatgcca tgaactacgt ttacaattcg cactacggcc agcagctgag
      901 cattttcgga agtgatcctg cagtgatcgc gaagatggta gatccactgg tgaatcaggt
      961 ttgtcgcgtg aatctgaata gccagtgcca gcgagggccg gatacatttc catttacggg
     1021 aagaaaggga tccgcagaag gaactctttc cgtatcggac gcgcttcgtt cattttcgat
     1081 tcgctctctt tttgcagcca aagaaagcgc tcagaacaaa gccatcattg aaacgattct
     1141 gcgagacgat acgtccagtt tcatttcgac tgattttctt ttttgaacat ggctattcga
     1201 gaagtagtgc aggcccgtgt tcgccagctc acagacgaaa gatctgtgct tcgcgttctt
     1261 cctgttcatg gcggaaaggg agtagggcct tttctttttc tggatcacat gggtccatgg
     1321 gaagtttcgc cagaagaaac gagcgatgtg ggccacatcc gcacattggc ctggccacag
     1381 tcacctatct atttgaagga agcattctgc atcgagacag cgtaggttcc gttcaggaaa
     1441 ttcatcctgg agatgtaaac tggatgaatg ctggaaaagg gatcgttcat tcggagagaa
     1501 gaccaccctc tcttgcgaat cgcgcgtatc gaatccacgg tctgcaggca tgggttgggc
     1561 ttccactttc gcaggagctg tctgaacctt tctttcatca tcacccgcgg cgcgacttgc
     1621 ctcatttcac aatcgacgga gtggagattt ctctcctggc aggaagtgca ctcggagtca
     1681 ctgcaccggc gcagacgttt tctgatttgt tttatatgga tcttcgcatt ccgcggggtg
     1741 gaagctggac ttttgttcag gatcacagag agtgcgctct ttatatagta gaaggcactc
     1801 tgaggatcca cgggcagaat tttgtcagtc actctctggt tgtgtttgaa ccgggaaagg
     1861 aagtgaagat ggatgcgcag gatggagtga ttgcagtgtt gctcggcggt gcgcccctgg
     1921 acggccctcg ccatatgtgg tggaattttg tttcttcttc gcgagatcgc atagaagaag
     1981 caaagcgatc gtgggcttcc ggaacatttg gggaaatcga aggtgagact ggatccattc
     2041 cgctgccttg aactcggaac gattttggaa ccagctaccg tcaggaaata tgttagaaaa
     2101 tatgttgacc tcgccagaaa agaaagcaac ggataaacct gaaatgtttg atactcttgg
     2161 tatctaccgc aggctgaagc gcgcccgcgc ttccgaacct gttgcgcgcg agatcgctat
     2221 ggccatggga gaaatcgtgc agagtcagct ggcaacgaaa gaggacctgc ttgtcgtcag
     2281 gagagatttc caggatgtga agcaagaact gaaattggag atatccgcgt cccggatgga
     2341 gactctgcga tggatgatgg gaatgatggt ggcccagctg gccttgcttg tctcaatact
     2401 taaaattctt taggaatgca ggggtagagt tgcatccgct tttgcggcgg atgcaacaat
     2461 agaactcatt tgttcatgaa tttttcgcag cccgccacat tgtggttctt gatatctgtg
     2521 cagcttgctt ttgccatggc ttccccgcat gctttagcgg cttccatgtc ttcttttgtg
     2581 aagttttctg gtttgtcttt ctgcggaacg gcagaggcct gcgccataca ggattctttg
     2641 gagaacttcg cagacatcat ctgacgcatg ttctcaggca tgcccttcat catttcttgc
     2701 atgcattctt ccatctttcc gcacatctgg gacttgatct tgtctgattc cgctttgtag
     2761 tcttctgctc cgccggacga cgaagatggt ttgctacagg caagagctgc caggagcaaa
     2821 aagggggtga ttgcaaagag ttttctcatg ataacctctg attttttgat aagatcatga
     2881 ttcgatttcc gcccgcgcca atcaatcaaa actcgaaatg tttgaaaaat catttgactg
     2941 aattcaccgt taggcggcaa cgaagggtcg atctgaaagg aggccgcgat ggataaggca
     3001 tttcgcctga gtcaactgat aggaaataca cctctcgtgg acctctcaag gaaagggtct
     3061 cgcgtccgtc tgttcggaaa gctgaagggc aacaatcccg ccggaagcgt gaaggacagg
     3121 cctgcgctat ggatgatgga acaggcggag cttcgaggag aattgaagcc cggaatgcgg
     3181 atcgtggaag cgaccagcgg aaatactggg atcgcgctgg caatggtggc agccgtcaaa
     3241 ggttacggaa ttgaattgat catgcctgag gatgcaacgc gagagagggt acttgccatg
     3301 caagcatacg gtgctacagt cacactcacc gatgcatcca gatctatgga aggcgccatc
     3361 gatcttgctc acgagcgctt cgcggaaggt ggtttctgga tgcccaacca gttcgcaaac
     3421 cctgacaact ggaaatcaca ctatcacacc accggtcctg aaatctggag agatacagag
     3481 ggaagagtga cgcattttgt ttcttccatg ggaactacag gaaccatcat ggggacgtct
     3541 cgctttttga aagagaagaa ccctgccatt caaatcattg gtgttcaacc ggaggatggt
     3601 gcgaagattc caggcattcg tcgctggccc gtcgaatact taccggccat ctttgagccg
     3661 gcgcgtgtgg atcgcgtaat ggatgtcact caacaagacg cagaagcaac cactcgcctt
     3721 cttgcgaaga gggacggtgt attctgcggc atgagttcag gcggagctat ctgcgccgcg
     3781 tcgcgactcg cagagcagtt agaagatgga gtgatcgtca cgatcctttg tgatcgcgga
     3841 gacaggtatc tctcctctga tttgttcgga tgattttcta aaccgattcg atcaccatgg
     3901 caatcccctg gcctcctcca atgcaaaggg aggcacatcc gtatttcagt tttcgcctgc
     3961 gcagttcctt tgccagtcca agtagaattc tatttcctga tgctccgagt ggatggccaa
     4021 tcgcgatggc accgccgttt acgtttgtaa tttccggatc cagtttcaat gattttgcaa
     4081 ccgcgaggta ttgagccgcg aacgcttcgt tgatttccca gagttggatt tgttcctgct
     4141 ggatgcccgc gcttgcaagt gcggccggaa tggcgtgcac cgggccaatt cccatttttt
     4201 ccggagggca cccgcgcgat gcccatccaa gaattttcgc cagtggcttt tttccggacg
     4261 actgcaaata acgttcggaa cagactacaa ctgcggaagc gccatcgttg atgccggagg
     4321 cgtttccagc tgtgacggtt ccgttttcga gaaatgcagg tgcaagcccg gccagcttcg
     4381 cgcccgccgc ggtgccgcga acaaattcat cgcgatcaaa tactttcatt tctttaccgt
     4441 ctttgacctg gacgcttttg atttcgtctg aaagcttacc tgatttctgt gctgcctctg
     4501 ctcgcgtttg tgagacgagt gcccaggcgt cctggtcctg ccgagagatc ccgtattcct
     4561 gggccagtgt ttccgcagtc cttcccattg cctggccaat gtatgggtct gtcaaagcgc
     4621 tggacaatag atcgatgagt ttcccgtctc cgtaacggat tcccgttctt gccccggctg
     4681 ccagataagg agactgactc atgctttcta caccgcacgc cagagataga tcttctcctg
     4741 cgcggatctg atgggctgcc tgggcgatcg cctcaaggcc gcttccgcag agtctattca
     4801 cattgagagc agatgcggag atgggcaggc ctgctttgag cccgacgtgg cgcgccaggt
     4861 agagagattc gggccccgag ggcactacat tgccgcagat aatggaagat acagccccat
     4921 gatcgacgga agcagcctca atggccgcct gcgccgccag gacacccagt tccaccgggc
     4981 ccagtcgcga aagactgccc aggaaggctc ccacaggcag tcttctgccc tctagaatat
     5041 atgctggatc tttcaatgtt ggcctgcctt tgcccgaaaa tcgtacagag tcgaaaaaat
     5101 ctggacgcct gtgagaaaaa acacgaattc tcgcagcacg agcgtaaaag tttctttttg
     5161 ggcagaccga tactgactaa tggttcggtc tacccgaagt agaggagaaa gggacatgga
     5221 tgttagaaaa agcggattcc tcgcaatctt tgcatcattg atgataaccg gtcaggtatg
     5281 ggcgcaggct caaccaacga ccacacaacc ggcacagcca gcaacgcagc ctggtcagcc
     5341 tgcacaaccg gcaacaccgg cggctgaaac agtaggcgct acaggaggac tcgaagtgat
     5401 cttgctcgat gacttcgaag agtctgaaga ctggcatgcg cgctccacgt gcccgctcgg
     5461 agaaaccacc acactgaaga tggttcagcg cggtgagatt cgcgcagccg atgacgagca
     5521 gtccaagcca ccagaaggcg aatcgctcaa cgttcaaaac aatccagaca acccgaacca
     5581 catcctcgga atcaagacct acttcgacaa ccgcggcttt gatcgtgtgg aagtgaaacc
     5641 acctcacgag tatgtaatca aaggtaaggc gcgtcaattc tccgtctggg tactgggccg
     5701 caagtttcgt cataacatga gcctgaaact ccgcgactat cgcggaaaca ctcacaacct
     5761 tccgcttggt cgtctggact tcttcggttg gagaaaactg actgtgaccg ttcctggatg
     5821 gctgccacag agcacccgtt atgcgctatt ggataagaac ctgcacttcg taagtctgtt
     5881 tgtcacgagc gatgtgcacg aagtaccggg ccagttctac ttctatgtag atggactgaa
     5941 ggctctcgtt gataaaagcg agaatgctta ccctggttca gaagtgaagg acaactggtg
     6001 aaaacgatga ttcgtgcgga gaaacctatg gaactgatta agaaaatctt cgatcgtcgc
     6061 acggcgacca tcgcagctgt gtcaagcatc gctgcagtgg ctctggcact tggcggcgga
     6121 atcgtaagtg cagacgtcaa aacggcagtt gggagtgacg tgtcttccaa tgaactgcga
     6181 tctatcacag tggaaagctg ggacaaggat tactcaaacg gcggctatgg ctgggaagtg
     6241 tccacagaca aggatatgac ttccaaagac ggccagtatc agcccaatgc tcaatctctt
     6301 caagctgaga gagaagtgaa gctgatcaaa ggtacacctc aggatattcg cgagaacgtg
     6361 aactatccag aagccagagt actcggtgtg aagtttggtt ttactttccc tggatacaac
     6421 gtggtgagca ttcgcccgcc gcatgtggat caatatgtgg tagagcgtcc tcgcccattt
     6481 ttgaacgagt atgctgtgac cggtgattac aaatcacgtt cctgctacaa gaatccagct
     6541 ctatcttctg tcaatgcagg tcgtgcgatg gtggtagatt gcctttatgg aatcgaactt
     6601 cctggtgaag ttcataaagt atctgtatgg gtgatgggcc gtggtaacga ctatgatctc
     6661 gaaggatggt ttgaagactg gaaaggtgct acccacattc tgaaattcgg ttctgtgaat
     6721 ttcattggct ggcgtcctat gtccattgag attcctaaga gcatccctca agatgtgaac
     6781 tctttccctg cgatcaaaac gctcgtgttc aagcaattca agcttcgttc caagccacat
     6841 acgtctctcg aaagcgttta catcttcttt gatgagcttc gcgttctgac ggacatcttc
     6901 gaagttcatt tcgatggtgc ggctcttgac ttcgacaagg cggattgtga tcgtaaaaac
     6961 catctgctca agatgattcg cgacaatgca cgtaacccgg acgcttatac tgctctcgta
     7021 gattgcagca aggctccagg tcctgcagca cccatccaga gcggaaatac gcagccttca
     7081 cagcctgctc aaccagcaca gcccgctcaa ccgtaagcga gtggtctaag actgaacgaa
     7141 agccgcctcg aaagggcggc tttttttatg gcctgaacag ggcaccagag tcgtcagata
     7201 gaacaatcct cgcaccagca tcgcgtttga attagggtgt gaagggcg
//
LOCUS       DAOQJL010000148         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5712_length_7226_cov_3.518059, whole genome shotgun sequence.
ACCESSION   DAOQJL010000148 DAOQJL010000000
VERSION     DAOQJL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_7226_cov_3.518059"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..775)
                     /locus_tag="PLG78_08245"
     CDS             complement(14..775)
                     /locus_tag="PLG78_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23143.1"
                     /translation="MKHMIPGMILVLASCATTYNQFYLAQPVPSDRASRILITPIFIT
                     KSQIRILSKYGVKWEEAEDEQAFDTNFHFLTELLQRKTNHESELLTKEDRDELMDELE
                     VSGTPKLAERNEGARNFWNGKHCASCIKKLLDERNAKWLLVPSVMVSEFSGNYYLNGN
                     SYCPDRHWAGLRSQYLLFDTEGTLIMDSSQLTPSNFLTGVEPASVGIHISDLVVSRTQ
                     GETRQACRVVMRPKVTIAASYSRINYLLPGMEKPK"
     gene            complement(800..2383)
                     /locus_tag="PLG78_08250"
     CDS             complement(800..2383)
                     /locus_tag="PLG78_08250"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNE23144.1"
                     /translation="MKTQKKTLPFEITEPAFEIKVGKAPAGAILVMPMFKDQAVKTLP
                     LSGEGKSLLEEFVKSKTFQGKSSEICDLSARGLIVFGLGAKEAFHPDTIAGQMIHIAP
                     KLLKYEKSTYVLRFTEEVWQALKGFRDGKVEPAEDDESFSDYFAPMTDEELLSQCVSS
                     LLAGSDSMEMLKSKKKSAEKKTVVYVEAEYLKKKLADAVARGVAVGELSHSMRYVESL
                     PGNFFHPEAFQTYTEQLTRKHGVSLKVFGMSDLEKMGCGGIVSVGKGSRIPPRMMILE
                     YKPKNAKNKKPIVLAGKGITFDTGGISLKPAGEMHEMKYDMCGSAIVIHALALAARRK
                     LPVHITGIVAIAENMPGGNAIKPGDVFTAYNGVTVEIQNTDAEGRLVLADILSYACAH
                     LKPGVLFDFATLTGACVVALGHETAGVMTPDDELADRIDRTSRRSLDRTWRLPHWRQY
                     GQGLKSEVADLRNVSGRDGGTITAFRFLSKFVQRSVPWAHFDIAGIAWHSKGYGTRTK
                     GASGWGLAFLNRYFEDEMR"
     gene            complement(2380..3111)
                     /locus_tag="PLG78_08255"
     CDS             complement(2380..3111)
                     /locus_tag="PLG78_08255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE23145.1"
                     /translation="MAKKAKISPSKKSAKKSAPSKNKKSAKKAVKKSIKKSTKKSVKK
                     SVKKVIKKAPARKAPAKVAPAKKAPAVSAPSRTTAVPTPVAPQAPAVSYPDQIADRSV
                     RLTDGSTSSLSGLSGPKGLVLYFYPKDDTPGCTVEACNFRDAESKIRAMGYKIAGVSP
                     DTSTAHQKFTAKYHLNFPLIADEDHSLCEAAGVWQEKSMYGKSYMGVARTTLVLDPSL
                     NVKKVYEKVKPDGHADAIAGDLSGL"
     gene            3191..4156
                     /gene="trxB"
                     /locus_tag="PLG78_08260"
     CDS             3191..4156
                     /gene="trxB"
                     /locus_tag="PLG78_08260"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNE23146.1"
                     /translation="MSAIHSVVIIGSGPAGHTAAIYSARAGLSPAMYEGFMAGGVAAG
                     GQLTTTTEVENFPGFPEGITGPELMDRMRTQSQKYGTTIFTETIERVDFSQRPFKIYS
                     QNDPDTAILAHTVIIATGATAKTLPIGGFQDYWQKGISACAVCDGSLPIFKNVPLFVV
                     GGGDSACEEATYLSKFGSRTYLVHRRDKLRASQIMQERVLKNPRIEILWNSEIVEAAG
                     DGRKIGSVVVRNIASGETRKQDAGGLFFAIGHKPNTDVFKNILSMDEDGYLITKPNST
                     ATNIEGVFAAGDVQDKKYRQAITAAGSGCMAALEAERFLAATGAH"
     gene            4237..5475
                     /locus_tag="PLG78_08265"
     CDS             4237..5475
                     /locus_tag="PLG78_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNE23147.1"
                     /translation="MRHKQALSETSERRWNIVLDELRREIPPPLFRAFIAPVSVSCEN
                     EDLHIIAPDENKRRHLDKHYLTLLESAWRKQASTGRIRLLTLEKEREQLQERAQPIPV
                     SSHFDFLIHERVSPLVSEMLNLNFRAPLYFLHGETSTGKTELMRQIETRAKAAGQTVR
                     RTGLDGFIVEFAAACKRKSTVDFRKSWKDCRVVMIDDLQFLKPGAKQTREELRHLAEH
                     SLAGNLTLILAADVPLRQIHMEEDLRSRLAAGAHIQLPPPDRSMRIRLFERFCKEAGV
                     TPQESLSARAARSYKDPRALRSLALQLSVDPAAGDRIPAHEPEFSSILRHTAAFCGLP
                     ESEIMGDGQSRRTVGARRMAMVLVAELMNRTPTQIARLFNRSGHTSVLYAMARMKKDL
                     EEDLFIANQLAELRRLILAD"
     gene            5503..6282
                     /locus_tag="PLG78_08270"
     CDS             5503..6282
                     /locus_tag="PLG78_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNE23148.1"
                     /translation="MVKYTLLLLLLPFHLMAAMPPGVAPGATFDSRGHVWVLTANNQQ
                     VIWFENGVKKAEGPFEHNQKRGVWTHYYESGKIKGTGAYEANLKQGPWKIYHPEGKVE
                     SEGSFKNDARSGKWVYYDKMGNKSAEGEFRAGMKEGPWVSYYGNGQVASRGSYRAGEA
                     QGAWEYFFEKGQPFQSGQFDGDVRIGTWTICVAPQGPCGKEVYNSASKSGAPAVSGLN
                     PAELTSQEIRNAKGNPARVLESMEGDVPDAVPSSINQKWND"
     gene            6343..>7226
                     /gene="pyrB"
                     /locus_tag="PLG78_08275"
     CDS             6343..>7226
                     /gene="pyrB"
                     /locus_tag="PLG78_08275"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNE23149.1"
                     /translation="MFSHLHILDADQFSREDLETVLSSTDRIAQLLKENKTFGLLDGK
                     ILASLFFESSTRTRLSFETAMNRLGGRVVTTVGFQFSSISKGETLYDTMKMIEAYADI
                     AVIRHPVEGSSRIAAAAVNIPVINAGDGIGQHPTQALLDLYTIFKEKGAIEGLRIGFV
                     GDLKFGRTIHSLIKMLRHYNVEIVLISPVDLALPESYRKELRRENVKFEETEDLKALW
                     DCDVGYITRIQEERFADRSEFERLKDSYNVNRAFLLASRKPTTILHPLPRVAELSTDV
                     DDMPTAAYFRQAGYGVVIR"
BASE COUNT         1822 a         1880 c         1754 g         1770 t
ORIGIN      
        1 attttgccac ttgttatttc ggtttctcca tcccgggcaa tagatagttg attctactat
       61 aggaagcagc aatcgtcact ttgggtcgca tcaccactcg acaggcctga cgcgtctcgc
      121 cctgagtgcg actgacaacc aggtcggaaa tgtgaatgcc aacgctcgcg ggctccactc
      181 cagtaagaaa attggaggga gtcagctggc ttgaatccat gatcaaagtc ccttccgtgt
      241 caaaaagaag atactgactt ctcaagccgg cccagtgtcg atcaggacaa taggaattgc
      301 cgttcaaata atagttacct gaaaattcac tgaccatgac ggaaggaacc agaagccatt
      361 tcgcattccg ctcatcgagg agcttcttaa tgcaggatgc gcagtgtttt ccattccaga
      421 agttccgggc tccttcattc ctttcggcaa gcttcggagt cccgctcacc tccagctcgt
      481 ccatcagctc atcgcgatct tcttttgtta gcaattcact ttcatgattt gtttttctct
      541 gcaagagttc ggtcagaaaa tggaagttgg tatcgaatgc ttgctcatcc tctgcttcct
      601 cccacttcac gccatacttc gagaggatgc gaatctgtga tttcgtaatg aagattggtg
      661 taatcaggat acgagatgcg cgatcggaag gtactggctg agccagatag aactgattgt
      721 aggttgtagc gcacgaagct aaaactagaa tcatgcccgg tatcatatgc ttcattatga
      781 aagaaaaccc tcgccagtgc tatctcatct catcctcaaa gtaacgattc aaaaacgcaa
      841 gtccccatcc gctggcgccc ttcgtgcgag ttccataccc tttcgagtgc caggcaatcc
      901 ctgcgatatc gaagtgggcc cagggaacac ttctctgcac aaacttggat agaaaacgaa
      961 atgcagtgat agtgcccccg tctctcccag aaacgtttct caggtctgcc acttctgatt
     1021 tcaaaccctg gccatactgt ctccagtgag gcagtctcca cgttctgtct agagacctgc
     1081 gcgaagttct atcgattcta tccgcgagtt catcatccgg ggtcatcacc cccgccgtct
     1141 catgcccgag agcaacgaca cacgcacctg tgagcgttgc aaaatcaaag agaacccctg
     1201 gctttagatg agcgcaagcg taggacagaa tgtccgcaag aacgagcctg ccctccgcgt
     1261 ccgtattctg gatctccacg gtgacaccat tgtaagcagt gaatacatct cctggcttga
     1321 ttgcatttcc gccaggcata ttctccgcga tcgcaacaat tccagtgatg tgaaccggga
     1381 gcttacgcct tgctgccagg gccagagcat ggatgacgat ggccgatccg cacatgtcat
     1441 atttcatttc atgcatttca ccggccggct tgagagaaat tccccccgta tcaaaggtga
     1501 tgccttttcc cgccagaacg atgggtttct tattctttgc attctttggt ttgtactcta
     1561 gaatcatcat tcgtggtgga attctggatc ctttgcccac ggaaacaatc ccgccgcatc
     1621 ccatcttttc caggtctgac atcccgaata ctttcaggct aactccatgt ttgcgagtga
     1681 gctgctccgt atacgtctga aatgcctccg gatgaaagaa gttcccaggc aaggattcca
     1741 cataacgcat gctatgcgaa agctctccaa ctgcgacacc acgcgccacc gcatccgcga
     1801 gtttcttttt caaatactct gcttccacat acactacggt ctttttttca gccgatttct
     1861 tctttgactt gagcatttcc atcgaatcgg aacctgcaag caatgacgag acgcactgcg
     1921 agagaagttc ttcgtcggtc atgggcgcaa aataatccga aaatgattca tcgtcttctg
     1981 cgggctccac cttcccgtcc ctgaagccct tcagcgcctg ccagacctct tccgtgaacc
     2041 tgagaacgta ggtgcttttc tcgtacttca gaagcttcgg ggcaatgtga atcatctgcc
     2101 ctgcgattgt atccgggtga aatgcttctt tcgctcccag accaaataca atgagcccgc
     2161 gcgcagacaa atcgcaaatt tcagaggact ttccctgaaa cgttttggat tttacgaact
     2221 cttccaggag agactttcct tctcccgaca gtggcaaagt ctttactgcc tggtctttga
     2281 acatgggcat tactagaatg gcgccggcag gagcttttcc caccttgatt tcaaaggcag
     2341 gctcggtgat ttcaaagggc aatgttttct tttgtgtttt cacaggcctg agagatctcc
     2401 agcgatcgca tctgcatgtc cgtccggttt cactttttca taaactttct taacattcaa
     2461 cgacggatcc agtaccaacg tagttcgcgc cacgcccata tacgattttc cgtacatgct
     2521 cttttcctgc cagactccgg ctgcttcgca cagcgaatga tcttcgtctg cgatgagcgg
     2581 aaaattcaaa tggtactttg ctgtaaactt ctggtgagct gtggaagtat ccggagagac
     2641 tcccgcaatc ttgtaaccca tggcgcggat ctttgattcc gcatcacgga agttacacgc
     2701 ttctaccgtg caccctggag tgtcatcctt gggatagaaa tagagaacga gtccttttgg
     2761 accggacaaa cccgaaaggc tcgatgtgct tccgtctgta agtcgcacag atctgtctgc
     2821 gatttgatcc gggtagctga ctgcaggtgc ctgtggagct actggagtgg gaacggctgt
     2881 tgtcctggaa ggtgcactta cggctggcgc tttctttgcc ggtgctacct tcgctggagc
     2941 ctttctggca ggtgctttct tgatgacttt ctttaccgat ttcttaaccg actttttggt
     3001 agacttctta atcgattttt taacggcctt ttttgccgat ttcttgtttt tgctcggggc
     3061 ggactttttc gcgcttttct tggaggggga aatctttgct ttctttgcca tagcacttac
     3121 tacaaaatcg caccgcacga gtcctgaaaa tttttatttg atcggcagac ggtttttgtt
     3181 ttctgactgc atgagcgcga ttcactcagt tgtcatcatt ggaagcggac ctgccggaca
     3241 cacggcggcc atctactctg cccgggccgg gctgtctccc gccatgtatg aaggattcat
     3301 ggcaggtgga gttgccgcag gcggtcagct gaccacaact acagaggtgg agaactttcc
     3361 aggcttcccg gaaggaatca caggcccgga actcatggat agaatgcgaa ctcaatctca
     3421 gaaatacggc actactatct tcacagaaac catagaaaga gtagatttca gccagagacc
     3481 tttcaagatc tatagccaga acgatccaga cactgccata ctcgcccata cagtcatcat
     3541 tgccacgggt gccactgcga aaactcttcc gatcggtggc ttccaggatt actggcaaaa
     3601 gggaatttca gcatgtgccg tatgcgatgg ctcactgcct atttttaaga acgttcctct
     3661 cttcgttgta ggtggtggag attctgcatg cgaagaagcc acctatcttt caaaattcgg
     3721 ctcgcgcacg tacctggtcc atagaagaga caaacttcgc gcatcccaaa tcatgcagga
     3781 aagagttttg aaaaacccgc gcatagagat tctgtggaat tcagaaattg tagaagcggc
     3841 aggagacgga agaaaaatcg gaagcgtggt agtgcgcaac attgcaagcg gagaaacacg
     3901 caagcaagat gcaggcggtt tgtttttcgc aatcggccac aaaccaaata cggacgtttt
     3961 caagaatatc ctcagcatgg acgaagatgg atacctgatc acaaaaccaa acagcactgc
     4021 aaccaacata gaaggtgttt tcgcggccgg tgatgttcag gataagaaat acaggcaggc
     4081 aatcacagct gcaggaagtg gctgtatggc cgctctggaa gcagaaagat ttctcgcagc
     4141 caccggcgcg cactgatcac ttgatttagc ttgcgtcgct ggaagaatca ggcgacaatg
     4201 tccgatcgca ctttttctta tgcgacataa tcacggatga gacataaaca agcattatcg
     4261 gagacatcgg aacgcagatg gaacatcgtt ctagatgaac tgagaaggga aattcctcct
     4321 ccactctttc gtgcgttcat tgcacccgtt tcggtttcct gcgaaaatga agatcttcat
     4381 atcattgcgc cggatgaaaa taagagacgg cacctggaca aacactacct gactctactg
     4441 gaaagcgcgt ggagaaagca ggcgtccaca ggcagaatca gacttctcac tctggaaaaa
     4501 gagagagagc aacttcagga aagagcgcag cccatccccg tttcctctca ctttgatttc
     4561 ttgattcacg aacgtgtttc ccctcttgtt tcagaaatgc tgaacttgaa tttcagagct
     4621 ccgctttact ttctgcacgg agaaacatcg acgggcaaaa ctgagctcat gcgacagatt
     4681 gaaactcgtg cgaaggcagc gggtcagact gtccggcgaa caggcctgga tggattcatc
     4741 gttgaatttg cagcagcgtg caaacgcaag tccacagtag acttcagaaa atcctggaaa
     4801 gattgccgtg tcgtcatgat tgatgatctg caattcctga aaccaggcgc aaaacagaca
     4861 cgcgaagaac tcaggcacct ggcagagcat tctctcgcag gaaatctcac actgattctt
     4921 gcagcggatg tcccgctcag gcaaatccat atggaagaag atcttcgctc tcgcctggca
     4981 gcgggcgctc acattcaact tcctcccccg gatcgctcca tgcgaatcag gctttttgaa
     5041 cgattctgca aagaggcagg agtgacgccg caagagagcc tctccgccag agcggccaga
     5101 tcctacaaag atccaagagc ccttcgaagc ctggccctcc agctcagcgt ggacccggcg
     5161 gcaggggatc gaatcccggc acacgaaccg gagttttcct ccattttgag acatacggcc
     5221 gccttctgcg gcttgcctga atccgaaatc atgggagacg ggcaaagcag acggactgtg
     5281 ggggccagga gaatggccat ggttcttgtc gcagaattga tgaatcgcac tcctacacag
     5341 atcgcgaggc tattcaatcg cagcggccat acgagcgtcc tgtacgcgat ggccagaatg
     5401 aagaaagacc tggaagaaga tttatttatt gcgaaccagc tggcagagct cagacgtctt
     5461 atacttgcag attgagtttt ttctgcaatt accgatcatc atatggtgaa atacaccctt
     5521 ttgctcttac ttttgccttt ccacctcatg gccgccatgc ctcctggcgt ggctccgggg
     5581 gccacatttg attcccgcgg acatgtatgg gtcctcaccg caaacaacca gcaagtgatc
     5641 tggtttgaga acggtgtgaa gaaggcagag gggccttttg agcacaatca gaagcgcgga
     5701 gtctggactc attattatga atcgggcaag atcaaaggga caggcgctta cgaagccaat
     5761 ttgaaacagg gcccgtggaa gatctaccac ccggaaggga aagtggaatc agaaggatcc
     5821 ttcaaaaacg atgcgcgcag cggtaagtgg gtctactacg acaagatggg aaacaaaagc
     5881 gcggaaggcg aatttcgcgc aggaatgaaa gaaggtccgt gggtttctta ctatggaaat
     5941 ggccaggtgg ccagccgcgg gagctatcgc gcgggtgaag ctcagggagc atgggaatat
     6001 ttttttgaaa agggacagcc attccagagt ggccaattcg atggagacgt gcgcattgga
     6061 acctggacca tctgtgtcgc tcctcaaggt ccttgcggaa aggaagtgta caactcagcg
     6121 tctaagtcag gcgcgcctgc agtatccggc ctgaaccccg cagaactcac ttcgcaggaa
     6181 atcagaaatg caaaggggaa tcctgcccgc gttctggaat ccatggaagg agatgtgcca
     6241 gacgcagtgc cctcttcgat caaccagaag tggaacgact agcttttttc tagcaggtga
     6301 tttttcactt ttcttcgtga gcctgaatgg ctcagtggac tcgtgttcag ccacctgcac
     6361 atcctcgacg cagatcagtt ttcgcgcgag gatctagaga cagtactgag ttccactgac
     6421 agaattgccc agctgctcaa agaaaacaaa acgttcggcc tgcttgacgg caaaatcctc
     6481 gcatctttat ttttcgaatc ttccacgaga accagactca gttttgaaac agcgatgaat
     6541 cgtctgggtg gccgcgtagt taccaccgtt ggttttcaat tctcctccat cagcaaagga
     6601 gaaactctct acgacacaat gaagatgatt gaagcgtatg cggacattgc cgttattcgc
     6661 catccagtgg aaggatccag tcgcatcgcc gcggccgcgg taaatatccc cgtaatcaat
     6721 gccggagacg gaatcggaca gcatccaaca caggctcttc tcgatttgta taccatcttc
     6781 aaagaaaaag gtgcaattga aggactgcga attggatttg tgggtgattt gaaatttgga
     6841 agaaccattc acagcctgat caagatgctg cgccactaca atgtagagat tgtcttgatt
     6901 tcgcctgtgg acctcgcatt gccagaatcc tatcgcaaag aactgcgacg cgaaaatgta
     6961 aagtttgaag aaacagaaga cctcaaagct ctctgggatt gcgatgttgg atacatcacg
     7021 cgcatccagg aagaacgctt cgcggacaga agcgaatttg aacggctcaa agactcctac
     7081 aatgtcaatc gcgccttcct tcttgcttct agaaaaccaa ccacaatcct ccacccactt
     7141 cctcgcgtgg cagaactctc aacagacgta gacgatatgc caaccgccgc ttactttcgc
     7201 caggcaggct atggagttgt gattcg
//
LOCUS       DAOQJL010000149         7197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5747_length_7197_cov_3.781014, whole genome shotgun sequence.
ACCESSION   DAOQJL010000149 DAOQJL010000000
VERSION     DAOQJL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_7197_cov_3.781014"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLG78_08280"
     CDS             complement(<1..1182)
                     /locus_tag="PLG78_08280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE23150.1"
                     /translation="MVFSSLIFLYVFLPVCIALYYVWQNRVWQNWVLIVASLIFYAWG
                     EPVWVVLLVLSSLVDYLNGLWIARFRGKAAAKLSVVCTLIFNLGILAIFKYQAFFIHN
                     LNLVSGLSIPVPEHHLPIGISFYTFQTISYVIDVYRDEVPAQKDFSKFLLFVSLFHQL
                     VAGPIVRYKDIAYEIENRIFSWNDFSYGVSRFAVGLAKKVVLANPAGEIAGSFLDGSP
                     ERMSVLGSWWGILLFTFQIYFDFSGYSDMAIGLGKMFGFTYKENFNYPYLARSATDFW
                     RRWHISLSSFFRDYVYIPLGGNRTRPLVSLTVVWFLTGLWHGASWNFVVWGLFYGALI
                     LFERAVQSVTGREIPRIIGHPYAILATLVGWTLFYFTDLGHAFAHLGRMFGVSGASLT
                     DP"
     gene            complement(1185..2267)
                     /locus_tag="PLG78_08285"
     CDS             complement(1185..2267)
                     /locus_tag="PLG78_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23151.1"
                     /translation="MREVRVVAFLFLIASGCAAPAAEEKLTRWDSSARLLAGMDLPES
                     DPLYSLTRTDEWQTHHRAMDRRFSEFEDMYGKKITPWAASEIGDSGAGVVFYPFGGPD
                     LLFPLRLFPDAKEYILFGMESPGKQPDPASVYARDRIALASVFPTIRKSLAWFLNFTY
                     FQTYQMDKWLRGRDLGGAGPVMMVFLARSGATITDVQEFQDPSAHGIRISFKRAGKDH
                     TATYYTVNASDSSLSSNPGFLAGIRSRGERVSYMKAASYLLYGPGFDIVRNFVTTESQ
                     MIVSDEAGVPLSSYPPEMELRFYGNYDGPLPMYWPMRQKALDQAYARESRSLPFDLGY
                     TASWKRNASVLIVASRKHATPGERKN"
     gene            complement(2264..2911)
                     /locus_tag="PLG78_08290"
     CDS             complement(2264..2911)
                     /locus_tag="PLG78_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23152.1"
                     /translation="MILDLHVYLRQRIWTCAACFLFLLSLCLPGSADAQEFFHYRIFT
                     PESGDLRELPDSSSESLATLSRGVALMETGMRDRQIVVQGKDGDWIQVAYFGDVGWVP
                     LNHTALPASIRTVSTSAEFLGTWRSNLKCRIQSYIHIEADGMFYGSHCISCTMEGCPE
                     SISGKWTIEDGMLVLTERKGQKAQYFIKDGVLVSDPTNGFIWEYHGASVLSGLRK"
     gene            complement(2908..3342)
                     /locus_tag="PLG78_08295"
     CDS             complement(2908..3342)
                     /locus_tag="PLG78_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNE23153.1"
                     /translation="MDLSSLPPINATLNGLSFLLQVSGYIAIRTGRRSLHRALMLSAV
                     TVSAIFLTSYLYLHAHVGSIPFGGQGFARVAYFSILISHSILAACTLPLVITTVTLAL
                     RGSVKHPKWGRVTILVWGYVSVTGVIVFLMMLPYYPRAFSPG"
     gene            complement(3342..3548)
                     /locus_tag="PLG78_08300"
     CDS             complement(3342..3548)
                     /locus_tag="PLG78_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23154.1"
                     /translation="MKTIFALISFALAVVPAELFACGACIGRGYSQSLIDSYVVITIL
                     LSGLPLIFGGLLIYYMVKRSKSGQ"
     gene            complement(3545..4066)
                     /locus_tag="PLG78_08305"
     CDS             complement(3545..4066)
                     /locus_tag="PLG78_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNE23155.1"
                     /translation="MDSTGRQNNVFFGMMLLLTTEAMFFSGLISAYLVARGNAAVWPP
                     ADQPRLPMFITAINTMILVLSGLTAVVSFRKKSVRLMILAAVLGLLFLLIQGSEWVRM
                     LSFGLTTVSSIYGAFFYLIVGAHGIHVAAGLLLLLLLLWKTKSGSVWIAGTIYWAFVV
                     AIWPVLYFLVYIL"
     gene            complement(4067..4384)
                     /locus_tag="PLG78_08310"
     CDS             complement(4067..4384)
                     /locus_tag="PLG78_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNE23156.1"
                     /translation="MSDHQQQNGHEDYHGHPNYFVIWAVLVVILGISLGLGYLGNHTL
                     AVTLIFALAFVKAFLVLANFMHLRWEPKAVTAIIIFSMIVLGYVFLGVYPDIVPIERI
                     IAQ"
     gene            complement(4400..4975)
                     /locus_tag="PLG78_08315"
     CDS             complement(4400..4975)
                     /locus_tag="PLG78_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNE23157.1"
                     /translation="MSSVATNQKVEFGRVPLGRIAMWWVIASEVVIFGGLITCYLLYR
                     IRYPEWGHEAAHTNTWLGALNTFVLLTSSFTIVLAHAASTKGELKKVWLYSLCTILGG
                     FIFLGVKAVEYTTEIHHGFTITKSVFWSFYYVMTGLHASHVIAGMTAIFVVALKARKG
                     EHLHRVEMAGLYWHFVDVVWIFLFPLLYIAK"
     gene            complement(5005..6693)
                     /locus_tag="PLG78_08320"
     CDS             complement(5005..6693)
                     /locus_tag="PLG78_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE23158.1"
                     /translation="MSHAMHAAPTSFLRKYVFATDHKVIGMQYLITGILMALVGGFLS
                     YLFRMQLAFPGESIPGFGKLAASQYNIAITMHGTIMVFWVAMPILLAAFGNFLIPLMV
                     GADDMAFPTLNMMSYQVFFISTVILLVSFFVPGGAFAAGWTAYPPLSAGTYAGSKDFL
                     SNMGGHLWIIAIALEFIAFLMGGINFLVTSFNMRAPGLSWFRLPVVVWMLDLAVLLFM
                     FSIGPLLAGAIMLLFDRTVGTGFYDPAQGGDPILFQHLFWFFGHPEVYVMLLPTLGFL
                     CEILATFARKPIFGYKYILYFTFGAALLSFIVWAHHQFVSGINPRMANFFSLTTLLIS
                     VPFTVIVFASIATLWRGNIQFSVPMLWAVGFVAEFMIGGVTGVYLGSSAFDIYAHDTY
                     FVIAHFHYALIPTVVFGGFAAIHFWYPKFMGRMPNETLGKWHFWITAIAFNGIFLPLF
                     FTGLMGEHRRIFDYSAWPSLMTDEMKLYRKFATTSLLVLLAAQPLFFINMIYSYFKGA
                     WAVNNPYKANSLEWSVPSPPPHGNFTEVPTVYRGAYEFSVPGREADYWPQDAKS"
     gene            complement(6710..>7197)
                     /locus_tag="PLG78_08325"
     CDS             complement(6710..>7197)
                     /locus_tag="PLG78_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE23159.1"
                     /translation="PALRLTQDAVPGRFIHGWFNATKPGKYEIACGQICGAGHTGMRG
                     MLIVHNKEEFQKWVENPVTGGSTDPMEILKGNGCTSCHSVDGTRVVGPSYKGIWGRSE
                     TVITDGAERQVVVDEAYIKKSIREPKADTVKGYPAGVMPAGTLSDADIDVLIKYFKTL
                     K"
BASE COUNT         2058 a         1792 c         1810 g         1537 t
ORIGIN      
        1 cggatccgtg aggctcgctc cggacacacc aaacattcgc cccagatgtg caaaggcgtg
       61 ccccagatct gtgaagtaaa aaagagtcca gccaacgaga gtggccagga ttgcatacgg
      121 atgcccgatg attcgcggaa tttcccgtcc ggtcacggac tgaaccgctc gctcaaaaag
      181 aatgagcgct ccatagaaaa gtccccatac tacaaaattc cagctcgccc catgccagag
      241 cccggtcaag aaccacacta ctgtgagact caccagaggc cgtgtgcgat tgccaccgag
      301 tggaatgtat acgtagtcgc gaaagaagga actgaggctg atgtgccagc gtctccagaa
      361 atcagtggca gagcgcgcga gatagggata gttgaagttc tctttgtaag taaaaccaaa
      421 catttttccc agaccgatcg ccatgtccga ataaccggaa aaatcaaaat agatctgaaa
      481 cgtaaaaagt aaaattcccc accaggaacc aagcacactc atgcgctccg gagatccgtc
      541 gagaaacgag cccgcgatct ctcctgccgg gttggccagt accactttct tcgcaagacc
      601 cactgcaaat cggctcacgc cgtaagaaaa atcattccat gaaaaaattc gattctcgat
      661 ttcgtatgcg atgtctttgt accgtacgat tggtccggcg acgagctgat gaaatagact
      721 gacaaagagc aagaattttg aaaaatcttt ctgcgccggc acttcatctc gatatacatc
      781 tatgacgtag gaaatcgtct ggaatgtgta aaaagatatc ccgataggca gatgatgctc
      841 aggaacagga atcgaaagac cgctcaccag gtttaggttg tgaatgaaaa aagcctgata
      901 cttaaaaatt gcaagtattc ccagattgaa gatcaaggtg catacaacag aaagttttgc
      961 agcggccttt ccgcgaaaac gcgcgatcca cagcccgttg agataatcta ccagagaaga
     1021 aagtacaagg agcactaccc agacaggctc accccaggca tagaagatca aagacgcgac
     1081 aatcagaacc cagttctgcc agactcggtt ttgccagacg tagtaaagtg caatgcaaac
     1141 aggaagaaat acgtatagaa agatcaggct agaaaatacc atgatcagtt cttcctttca
     1201 ccgggtgttg cgtgttttcg cgaagccaca atcagcacag atgcattcct cttccaggag
     1261 gcagtgtagc ccagatcgaa tggaaggctg cgactttctc ttgcatacgc ctgatcgaga
     1321 gccttttgcc gcatgggcca gtacatggga agaggcccgt cgtagtttcc gtagaatctc
     1381 aattccattt cgggaggata ggaagaaaga ggcactcccg cttcatccga aacaatcatc
     1441 tgcgattcgg tcgttacaaa atttctaacg atatcaaagc ctggcccata gagaagatag
     1501 gaagccgcct tcatatacga cactctctct cctctagagc gaatgcccgc cagaaaaccc
     1561 gggttggaag agagagaaga atcagaagcg tttactgtgt agtaggtggc cgtgtggtct
     1621 tttcctgctc ttttgaatga aatgcgaatt ccatgggcgg atggatcctg gaattcttga
     1681 acgtctgtga tggtagcacc actccgggcc agaaagacca tcatcacggg ccctgctcct
     1741 cccagatcgc ggccgcggag ccatttgtcc atttgatagg tctgaaaata ggtgaaattc
     1801 aaaaaccatg cgagtgactt gcgaatcgta ggaaacacag aagcaagcgc aatacgatcg
     1861 cgcgcgtaga cggatgccgg gtccggctgc tttccgggag attccattcc aaatagtata
     1921 tattctttag catccggaaa aagcctgaga ggaaacaata agtcgggtcc accaaatgga
     1981 tagaacacca ctccggcacc cgaatcacca atttcagagg cagcccaggg tgtgatcttc
     2041 ttgccgtaca tatcttcgaa ctcagaaaat cgtctgtcca tggcgcggtg atgcgtctgc
     2101 cattcatctg tgcgagtgag cgagtaaaga ggatccgact ctggaagatc cattcctgca
     2161 aggagccgcg cagacgaatc ccagcgagtc agtttctctt ccgcggcggg tgcggcacaa
     2221 ccagaggcga tgagaaaaag aaacgcgacg acacgtactt ctctcatttg cgcaaccctg
     2281 aaaggacact ggcgccgtga tattcccaga tgaatccatt ggttggatca gatacaagaa
     2341 caccgtcttt gatgaagtac tgtgctttct ggccttttct ctcggtgaga acaagcattc
     2401 cgtcttctat cgtccatttt cccgaaatgc tttccggaca accttccatg gtgcaggaaa
     2461 tgcagtgcga accataaaac attccatcgg cttctatatg aatataactc tgaatcctgc
     2521 acttcaagtt gctacgccag gtgccaagaa actccgcgga agtagagact gtgcgaatgc
     2581 tcgctggcag tgcggtatga ttgagaggaa cccagcccac gtctccgaag tatgctacct
     2641 gaatccagtc tccgtccttt ccctgaacaa cgatttgcct gtcgcgcatt ccggtttcca
     2701 tgagggccac accacgactg agagttgcca gggactcgga agaagaatcg ggaagttcgc
     2761 gcagatcgcc ggattcaggg gtgaagattc tgtaatgaaa gaattcttgt gcgtccgcag
     2821 atccaggcag acaaagcgag agcaaaaaca agaagcatgc agcgcacgtc cagatgcgct
     2881 gacgcaaata gacatgcaaa tccagaatca tccaggagaa aaggcgcgag gatagtaggg
     2941 aagcatcatc aagaacacga tgacccccgt aaccgacaca tacccccaga ctagaattgt
     3001 aacgcggccc cactttggat gtttgacgga tccgcgaagc gcgagggtaa ccgttgtgat
     3061 cacaagcgga agagtgcatg ctgcgagaat tgaatgactg atcaaaatgc taaagtaggc
     3121 gactcgtgca aatccctggc cgccaaacgg gatgctgccc acatgcgcat gcaaatacag
     3181 ataactggtc aagaagatcg cggatacggt aacggcagaa agcatcagcg cgcgatgcag
     3241 actacgacga cctgtgcgaa tcgcaatgta cccgctcacc tgaagtagaa aggaaaggcc
     3301 attgagagtt gcattgatgg ggggaagaga actgagatcc atcactgtcc tgacttggat
     3361 cgtttgacca tgtagtagat cagaaggccg ccaaagataa gtggaagacc gctgagcaag
     3421 atcgtaatga ctacataaga atcaatcagc gattgactgt acccgcgacc gatgcaagcg
     3481 ccgcaggcaa acaactctgc cggaaccact gccagtgcaa atgagatgag cgcgaaaatc
     3541 gtcttcatag tatgtacacc agaaaataca aaactggcca aatggcgacg acaaaggccc
     3601 agtaaatcgt tcccgcaatc cacacagatc cactcttagt cttccagaga agcagaagca
     3661 aaaggagcag gccggctgcc acgtgaatcc catgtgcccc gacgatcaaa tagaagaacg
     3721 ctccgtagat acttgatact gtggtcagac caaaggaaag cattcgcacc cattccgatc
     3781 cctgaatcaa gaggaacaga agcccgagta cggcggcgag gatcataagg cgaaccgatt
     3841 tcttgcgaaa gctaactaca gccgtgagac cactgagcac cagaatcatt gtattgatcg
     3901 cagtgatgaa catgggcaac ctgggctggt ctgccggagg ccaaacggcc gcgttgccgc
     3961 gtgcaacgag ataggcgctg atgagaccgg aaaagaacat tgcttctgtg gttagaagca
     4021 acatcattcc aaaaaagacg ttgttctgtc ttccggtcga atccatttac tgtgctatga
     4081 tcctttcaat gggaacgatg tctggataga caccgagaaa gacatatcct agaacgatca
     4141 ttgagaagat gatgatcgca gtgactgcct ttggttccca ccgaagatgc atgaaattcg
     4201 cgagcaccag gaatgccttc acgaaagcca gagcgaagat cagcgtcact gccagagtgt
     4261 gattgccaag gtatccaagc ccaagactga tccccaggat gactaccaga acggcccaga
     4321 tcacaaaata gttgggatga ccgtgataat cttcgtgtcc gttttgctgc tgatgatccg
     4381 acatttgctt ctccagaaat tattttgcaa tgtaaagaag tggaaacagg aaaatccaga
     4441 ccacgtcaac aaagtgccag tacaggcctg ccatttcgac gcgatgcaga tgttcccctt
     4501 tgcgtgcttt gagtgcgacc acaaagatgg ctgtcattcc tgcgattacg tgcgacgcgt
     4561 gaaggccagt catcacgtag taaaagctcc agaaaacact cttggtgatt gtgaagccgt
     4621 gatggatttc agtcgtgtat tctaccgcct taacgcccag gaagatgaaa cccccgagaa
     4681 ttgtgcagag agaatagagc cacactttct tcagctctcc ttttgtggaa gccgcgtgag
     4741 ccagaacgat cgtgaaactg gacgtaagca gaacgaaagt gttcagcgcc ccaagccagg
     4801 tgtttgtgtg agccgcttcg tgaccccact ccggatagcg gattcgatag agaaggtagc
     4861 aggtgatgag cccaccgaag attacaactt cggatgcaat cacccaccac attgcaatgc
     4921 gtcccagagg gacgcggcca aattcaactt tttggttagt ggcaacagaa ctcattttat
     4981 catatctcca gtttggtttg ccttttagct cttcgcatct tgtggccagt agtcagcttc
     5041 gcgacctggc acactgaatt catatgcacc gcgataaacg gtaggcactt cggtgaagtt
     5101 cccgtgcggc ggaggggaag gaacagacca ctcaagcgaa tttgccttgt atggattgtt
     5161 tactgcccat gcacctttga agtagctgta gatcatgttg atgaagaaca gtggttgcgc
     5221 cgcgagcaac acgagaagag aggtggtggc aaactttcta taaagcttca tctcatctgt
     5281 catgagactt ggccatgcag aatagtcaaa aatacgacgg tgttctccca tcaggcctgt
     5341 gaagaagagc gggaggaaga taccgttaaa cgcaattgct gtgatccaga aatgccattt
     5401 acccagagtt tcgttgggca tccttcccat gaactttgga taccagaagt gaatggcggc
     5461 aaaccctcca aacacaaccg ttgggatgag agcatagtgg aaatgcgcga tcacgaagta
     5521 cgtatcatga gcgtagatat caaacgcgga agaaccaagg tatacaccgg tcacaccgcc
     5581 aatcatgaac tctgccacga aaccaacagc ccagagcata ggcactgaga actgaatgtt
     5641 gccgcgccac agagtcgcaa tacttgcgaa cacgatgaca gtaaatggaa cagaaatcag
     5701 cagagtcgtt aagctaaaga agttagccat gcgaggattg attccggata caaactggtg
     5761 gtgtgcccat acgatgaagg acagaagagc cgcaccaaag gtgaaataga ggatgtactt
     5821 gtaaccgaag attggctttc tcgcgaaagt tgccagaatc tcgcacagga atccaagagt
     5881 gggaagaagc attacgtaca cttcaggatg accgaagaac cagaacaaat gctggaacag
     5941 aatcgggtct ccaccctgag ccgggtcata gaacccagta cctaccgtgc gatcaaagag
     6001 aagcatgatg gcgccagcga ggagtggtcc aatcgagaac atgaagagga gaacggcaag
     6061 atccagcatc cacactacta caggaaggcg aaaccagctc aggcccgggg ctctcatgtt
     6121 gaaggaagta accaggaagt tgataccacc catgaggaat gcgatgaact caagggcgat
     6181 cgcaatgatc cagaggtgac cgcccatgtt gctcaagaag tcttttgaac cagcgtatgt
     6241 gcccgcactg agaggaggat acgcagtcca tccagcagca aacgctccgc ctggaacaaa
     6301 gaaagacacg agaagaatga ctgtactgat gaagaacacc tgatacgaca tcatgttcaa
     6361 cgtcggaaac gccatgtcgt ctgctcccac catcagcgga atcaggaagt tgccgaatgc
     6421 agccaggaga atcggcatgg ccacccagaa taccatgatg gttccatgca tggtgatggc
     6481 aatgttgtac tgcgaagcgg cgagcttacc aaatccagga atgctttctc ctggaaatgc
     6541 aagctgcata cggaagagat aggacagaaa gccgcccacc agcgccatca gaatccctgt
     6601 gatcaggtat tgcattccaa tgactttgtg atcggttgcg aagacgtatt tgcgcaggaa
     6661 gcttgtgggc gctgcatgca ttgcgtgact catttctttc tccctttcct tacttcagtg
     6721 tcttaaagta tttgattaga acatcgatgt ctgcgtctga aagagttccg gcaggcatta
     6781 caccggctgg atatcctttg accgtgtccg cttttggttc acgaatcgat tttttgatgt
     6841 acgcttcatc tacaaccacc tggcgctctg cgccatctgt gatcacagtc tctgatctac
     6901 cccagatacc tttgtaactt gggccgacga ctctggttcc gtccacggag tgacaggaag
     6961 tacatccgtt tcctttgagg atttccattg gatctgtaga gccacctgtt acaggattct
     7021 ctacccattt ttgaaactct tctttattat gaacgatcag cattccgcgc attcccgtgt
     7081 ggccggctcc acagatttgc ccgcatgcga tttcgtattt gccaggtttg gttgcgttga
     7141 accatccgtg aatgaaacga cctggaaccg catcctgagt gaggcgaagt gctggca
//
LOCUS       DAOQJL010000150         7179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5770_length_7179_cov_4.294076, whole genome shotgun sequence.
ACCESSION   DAOQJL010000150 DAOQJL010000000
VERSION     DAOQJL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_7179_cov_4.294076"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..939)
                     /gene="trpA"
                     /locus_tag="PLG78_08330"
     CDS             complement(115..939)
                     /gene="trpA"
                     /locus_tag="PLG78_08330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNE23160.1"
                     /translation="MMDKLKEYILKKRAQGPLYMPYLTAGDPDFASTVRFACGMIDAG
                     ADLIELGIPFSDPTADGPVIQAAMVRAMENADFSFERIFQCAREIHEARPDVPIVFLS
                     YMNPVFRGFAHKSDRPLEAFLAEAARAGIQGIVIPDLPADQPEANEFFRVSERFQIAQ
                     IFMSAPNTSPDRLNDLRKHGRGFIYHVTSYGVTGERSELPPEVKTQMKAVRESTGLPV
                     FAGFGFSRPDQADTVKAVADGVIVGSLHHRLIQEKGPGALEAICESTAAFVKALRG"
     gene            complement(936..2111)
                     /gene="trpB"
                     /locus_tag="PLG78_08335"
     CDS             complement(936..2111)
                     /gene="trpB"
                     /locus_tag="PLG78_08335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNE23161.1"
                     /translation="MGSEKSGFFGEFGGRYSPEVLMPAILELEEVYFKVRSKKRFQKK
                     WEQLQRDYIGRPSRLTYAARLSDEWGAQIYLKREDLNHTGAHKINNAIGQALLALEMG
                     KERIIAETGAGQHGVATATAAAHFGLKCTVYMGSEDVRRQKLNCYRMKLLGAEVIPVT
                     SGTSTLKDATNEAMRDWARNVRNTHYIIGSAIGPHPFPIIVADFQSVIGKESRKQMKK
                     GPGLPHAVIACVGGGSNAIGIFHGFLKDDVDLYGVEAGGRSDDPGENSASITFGEVGY
                     FHGTKSLFIQKEGGQIQGVHSVSAGLDYPGVGPEHAYLAKTGRVKYVRVRDDRALECF
                     QEVSRLEGIIPALESAHALAFARELARDAGKKARILVNLSGRGDKDVAEAASILGME"
     gene            complement(2141..3865)
                     /locus_tag="PLG78_08340"
     CDS             complement(2141..3865)
                     /locus_tag="PLG78_08340"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNE23162.1"
                     /translation="MRASSLLIHTLREPPADAVLASHKMMLRAGLVSKLGSGLYNMLP
                     MGLRVFRKIENIIREEMNAAGGQEFVMPILIPAELWETSGRWNAMGKEMFRIKDRHET
                     WNVLGPTHEESFTDAMKGILKSYRDLPVNVYQIHTKFRDEIRPRFGVMRSREFVMKDA
                     YSFHKDEECLGRTYQVMRKAYRNIFGRLGLETIPVEADTGTMGGSRSEEFMVPSEVGE
                     ETLLISAGKKYFSNQEKTPFVAGKVEKPPALVKSAKKHHTPNAKSIEDVARFLGVSSV
                     HLAKALIYRVGESYVLVFIRGDRAVNDVKLKNALGGEEFRAATEDEVRSLGFVPGFIG
                     PGHVPEGMRIIQDSSIESGRPYVVGANEVDHHRSGHVFEANPDARDVALAVEGDRSPM
                     GDGVLHAIKGIEVGHIFQLGTKYTKAFGVSVLDESGRPLTPIMGCYGIGVQRTMAAII
                     EQNHDEKGIAWPVSACPFEVVLVSITKGEEEEQKVAALYQKLQKAGLDVLWDDRDLRP
                     GVKFADAELIGYPVRVTVGKTFFESGKLEIQLRKTGEKVETTPAEMVKTIRNLTRSLK
                     RKHPPKKR"
     gene            complement(3867..5471)
                     /locus_tag="PLG78_08345"
     CDS             complement(3867..5471)
                     /locus_tag="PLG78_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE23163.1"
                     /translation="MIALILGGALMLGICVFIHELGHYTMGRLVGVKAEIFSIGYGRG
                     IWKKKIGDTTWQVTAIPIGGYVKFYGDDISGNDKTPGGLFSVPPLTRIIPVLGGPLFN
                     LILGFGIFLLLHTISGPIAPRVDFWEETMQDSPAYRAGLRSGDSITAINGEAVHDFHD
                     LQKLVLLSGGADLTVDADRNGSKVQVKVKPEVDTAGRALIGLRAPGERNLKVNYPSRL
                     VWAYKLQSFLGHTELPAGLRAYPYLNNGDVILSVQGRNVKSATELQDLLGEFHGQPVQ
                     IRVKRQTLSWLAPWFTEETTVTVPSQAEYRIELHNLVDLKYGTPVADQVLLSNVEAHQ
                     RALSILSIDGKPPGSYEKMYEALATERAITLTLKDRNYSAKAHAAKIGLLGFRPDSRI
                     ETTYLPGHGSVSEVISHAFGDTAKNVMVYPAFFGSLISGRLSFIDNAMGPVGMFAAAG
                     VVLESGIQDYLGMFAAISIALFIMNLLPFPVVDGGHVVFFLYEAVRGKPVTPGIMEGI
                     YRGGLAVLVFMGLWIMYRDILFISGL"
     gene            complement(5482..6696)
                     /gene="dxr"
                     /locus_tag="PLG78_08350"
     CDS             complement(5482..6696)
                     /gene="dxr"
                     /locus_tag="PLG78_08350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNE23164.1"
                     /translation="MAFFEYSAQISAPARAKRTRKLILLGASGSIGSSTLSILPESGI
                     ELVAVSVHGKADRLAEIISKFPTIRYAAITDPEMAQGLARLSASFPSVQFFSGSSGLV
                     ELVRLGAQAGADTVLTAVMGSAGIAPTLEAIQIGLKIALANKETLVTAGPVIENALAL
                     SRACIVPVDSEHNALFQLLLGVEPEHLRRAILTASGGPFLSKSRADVEKVSRDEVLNH
                     PTWSMGPKITVDSAGLINKGLEIIEAHHLFGIPYSQLDVLIHPVSHVHAMVETPDGYR
                     MMASRPTMVFPIAHALYYPDPVPELGIATRPESWPPLEFRTVDTEQFPGFCLCMEAAR
                     AGGTAPAVLNASNEVAAGLFLSGLIQFTAIPHLIELALDRIPVQKGTETGLYLEADAR
                     ARALTSEMAREL"
     gene            complement(6703..>7179)
                     /locus_tag="PLG78_08355"
     CDS             complement(6703..>7179)
                     /locus_tag="PLG78_08355"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNE23165.1"
                     /translation="MTLCHAFLILAEERGLFFFVVVALATIGTDTGAYFAGRYFGKHN
                     AGLKVSPRKTIEGYVGGFVFSILLMLGFLYCWKRFAYEIHMGYVEMALFTAVLSVISV
                     FGDLVESAIKRDSKKKDSAALIPGHGGMLDRGDSLYFTIPIGYFYLYIRGIAGLTL"
BASE COUNT         1728 a         1958 c         1745 g         1748 t
ORIGIN      
        1 aaaagaacca gcggtagaag cagaaagagt gctgcagccg caaccagaag ccccagggca
       61 attttacgca taggagacga ttttggacct ccgccaacct ttcaacacgt atttttagcc
      121 ccggagagcc ttcacgaatg cagccgtcga ctcacagatc gcctccagtg cacccggccc
      181 tttttcctga atcaggcgat ggtggaggct gcccacaatc accccgtccg ccactgcctt
      241 aaccgtgtct gcctggtcag ggcgactgaa accaaaaccc gcgaatacag gaagtcccgt
      301 gctttcgcgg accgctttca tttgcgtttt tacctcgggc ggcagttcgg atctttcgcc
      361 agtgacgccg tagctcgtga cgtgataaat gaagccgcgg ccgtgtttgc gcaaatcatt
      421 cagtctatct ggagaagtat tgggtgcaga catgaagatc tgggcgatct ggaacctttc
      481 ggaaactcgg aaaaactcgt tcgcctctgg ctggtctgca ggcaggtccg gaattacaat
      541 tccctgaatt ccggcgcggg cggcctctgc caggaatgct tcaagaggtc tgtcgctctt
      601 gtgtgcgaag ccacgaaaca ccggattcat gtagctcaga aatacgatgg ggacatccgg
      661 acgcgcctcg tggatttctc tggcgcactg gaagatgcgt tcaaaagaga agtctgcatt
      721 ctccatcgcg cgcaccatgg ctgcctgaat gacggggcca tctgccgtag ggtctgaaaa
      781 aggaattcca agctcgatga gatctgcacc tgcatcaatc atcccgcagg cgaagcgaac
      841 cgtggaggca aaatcaggat cccctgcagt cagatacggc atgtagagag gcccctgggc
      901 tctcttcttc aatatgtact ctttgagttt atccatcatt ccatacccag aatagaagcc
      961 gcttctgcta cgtccttgtc accacggccg gaaaggttca cgaggatgcg cgctttcttt
     1021 cccgcgtcgc gcgccagttc ccgcgcgaaa gcgagtgcat gggcgctctc gagtgccggt
     1081 atgattcctt cgagcctgga tacttcctga aagcattcca gagcgcgatc gtctcggacg
     1141 cgcacgtact tcactctacc cgtctttgcc agatacgcat gttctggtcc gactcctgga
     1201 taatccagcc ccgcagatac gctgtgcaca ccctggattt gtccgccctc tttctgtatg
     1261 aaaagagact ttgtgccgtg gaaatagccc acttcgccaa aggtaatgct cgccgagttt
     1321 tcaccaggat catcgcttct gccgcctgct tccactccgt agagatctac atcgtctttc
     1381 aaaaatccgt gaaagattcc aattgcattg gatccgccac ccacgcatgc gatgactgca
     1441 tgaggaagac cagggccttt cttcatttgc ttgcgagact cttttccaat gactgattgg
     1501 aagtctgcta caatgattgg aaacggatga gggccaatgg cagagccaat gatgtaatga
     1561 gtgttgcgaa cgtttctcgc ccagtctctc attgcttcat tggttgcatc tttgagagta
     1621 gacgttccgc ttgttacagg aatcacttcc gcacccagaa gtttcatgcg ataacaattc
     1681 aatttctgac ggcgcacatc ttcgctgccc atgtaaacgg tgcatttcaa tccgaaatga
     1741 gcggctgctg tagccgtggc caccccatgc tgtccggctc cggtttcagc aatgatccgt
     1801 tcctttccca tttcgagagc gagcaatgcc tggccgatgg cgttgttgat cttatgcgct
     1861 ccggtgtgat tgaggtcttc gcgcttgaga tagatttgtg cgccccattc gtcgctgagc
     1921 ctggctgcat acgttagacg agatggtctg ccaatgtagt ctctttgcag ctgttcccac
     1981 tttttctgaa aacgcttctt actgcgaacc ttgaagtaga cttcttccag ttccaggatc
     2041 gcaggcatga gaacctcggg gctgtacctt ccaccgaatt ctccaaaaaa accgcttttt
     2101 tcagaaccca tgagccagcg ttggcgtcat gccctgcact tcatctcttt tttggcgggt
     2161 gctttcgctt gagcgatctg gtaagattgc gaatggtttt gaccatttca gcaggcgtgg
     2221 tttctacttt ttcgcccgtc ttacgcaatt gaatctctag ttttcctgac tcaaaaaaag
     2281 ttttaccgac tgtaacgcga acaggatatc caatgagttc tgcatctgcg aatttcacgc
     2341 ctggtctgag gtccctgtcg tcccacaaaa catcgaggcc cgctttctgt aacttctggt
     2401 agagcgccgc taccttttgc tcttcttctt ctccctttgt gatgcttaca agcaccactt
     2461 caaaaggaca ggcactcacc ggccaggcaa ttcccttctc gtcatggttc tgctcaatga
     2521 tggcagccat ggttctttga acgccaattc cgtaacaacc catgatagga gtgagaggcc
     2581 tgcctgattc gtctagaacg gacacaccaa atgcttttgt gtacttcgtt ccaagctgga
     2641 agatatgacc tacctcaatg cctttgatcg catgcagtac tccatcgccc ataggcgaac
     2701 gatccccttc tactgcgagt gccacatcgc gcgcgtctgg atttgcttcg aaaacgtgac
     2761 cgcttctatg atgatctact tcatttgctc ccactacata aggacgaccc gactcaatgg
     2821 acgaatcctg aatgatgcgc attccttctg gcacatgacc cgggccaatg aaacctggaa
     2881 caaaaccaag ggaccgcact tcgtcttctg tggcagcgcg aaactcctct ccacccaggg
     2941 cattcttcaa cttcacgtca ttgacagcgc gatctccgcg aatgaagaca agaacatagg
     3001 attcgcccac gcgatagatc agagcttttg ccagatgaac ggacgacaca ccgagaaacc
     3061 gggcgacatc ttctatggac ttagcattcg gagtatgatg cttcttcgct gattttacaa
     3121 gtgccggtgg cttctccact tttcccgcaa caaacggagt cttctcctga ttgctgaagt
     3181 atttcttccc ggccgaaatc aaaagcgtct cttcccctac ttcagaagga accatgaact
     3241 cttcggatct cgatccgccc atggttccag tatctgcttc tacgggaatg gtctcaaggc
     3301 caagcctgcc gaatatattg cgatatgctt tgcgcatcac ctgatacgtt cgacccagac
     3361 actcttcgtc cttgtggaac gaatacgcat ccttcattac aaattcgcgc gatcgcataa
     3421 caccaaaccg cggacgaatt tcatcgcgaa acttggtgtg aatctggtac acatttacgg
     3481 gaagatctcg ataggatttc aaaatccctt tcatcgcatc cgtgaaggac tcttcgtgtg
     3541 tgggtccaag aacgttccat gtttcgtgtc tgtctttgat tcgaaacatt tccttgccca
     3601 tggcattcca tctgccggat gtttcccaca gttcggccgg aatcaggatg ggcatgacaa
     3661 actcttgacc gcccgcggca ttcatctctt cgcgaatgat gttttcaatc ttgcgaaaga
     3721 ctcgcaggcc cataggaagc atgttgtaaa gaccactacc gagcttgctg accaggccgg
     3781 cgcgtagcat catcttgtgt gacgcgagta ccgcatccgc cgggggctcc cggagagtgt
     3841 gaatcaaaag agaacttgcg cgcattctaa agtccagata tgaagagtat gtcgcggtac
     3901 atgatccaca gtcccatgaa gacaagaact gcaagaccac cgcggtagat tccttccata
     3961 attccaggcg ttacgggttt accgcggaca gcttcgtaca gaaagaatac tacgtggccg
     4021 ccgtcaacaa cagggaacgg caaaaggttc atgatgaaaa gcgcaataga aatcgcagca
     4081 aacataccca ggtagtcctg aattcctgat tcaagcacta ctccggccgc agcaaacatc
     4141 ccgacaggcc ccattgcgtt atcgatgaaa gaaagtctgc ccgaaatcag gcttccaaaa
     4201 aatgcaggat acaccatgac gttcttcgca gtatcaccaa aggcgtgcga aatcacttcg
     4261 ctaacggaac catgccccgg aagatacgtg gtttcaatgc gcgaatccgg tctaaatcca
     4321 agaaggccaa tttttgcggc gtgcgccttc gcagaatagt tgcgatcctt tagtgtcaga
     4381 gtgatggcac gttctgttgc cagagcttcg tacatttttt cgtaagaacc aggaggtttt
     4441 ccgtctatag aaagaatgct gagtgcacgc tggtgtgctt ccacattgct gagcaatacc
     4501 tgatctgcca cgggagttcc gtacttcaag tcgacaagat tgtgcagttc aattctgtat
     4561 tctgcctgcg atgggactgt tacggtggtt tcttctgtaa accacggagc tagccacgac
     4621 aacgtttgtc tcttcacacg aatctgcaca ggctgtccgt gaaattctcc gagcaaatcc
     4681 tggagttctg ttgcggattt tacatttctt ccctgcacgg aaagaatgac atctccgttg
     4741 ttcaaataag ggtacgccct gagtccagcc gggagttcag tatggcccag gaaggactgg
     4801 agcttgtacg cccacaccag gcgagacgga taatttactt taagatttct ttctccgggt
     4861 gcgcgaagac caatgagagc gcggcctgcc gtgtctactt ctggcttgac cttgacctga
     4921 actttgcttc cgtttctatc tgcatccaca gtcagatcgg caccgcctga tagaagcacc
     4981 agtttctgca aatcgtgaaa gtcatgcacc gcttctccgt tgatcgcagt gatggaatct
     5041 cctgaccgaa ggccggccct gtaggctgga ctgtcctgca tggtttcttc ccagaaatca
     5101 acgcgcggcg cgataggacc ggagatcgtg tgaagtagaa gaaagatacc aaaaccaaga
     5161 atcaaattga agagcggtcc gcccagcact ggaatgattc gcgtgagagg aggaacagag
     5221 aaaagtccac ctggagtctt atcgttgccg gagatatcat ctccgtagaa tttcacatat
     5281 ccgccaatcg ggatcgcggt cacctgccag gtagtatctc ctattttctt tttccagatg
     5341 ccgcggccat aaccaatgga gaaaatttct gccttcactc ccacgagtcg gcccatggtg
     5401 tagtgaccca gttcgtgaat aaagacacag atgccgagca tgagagctcc gccaagaatg
     5461 agtgcgatca tacagcctct tttacagttc ccttgccatc tctgatgtca gcgctctggc
     5521 ccgggcatcc gcttccagat agaggcccgt ttccgttcct ttctgtaccg ggatgcgatc
     5581 cagtgccagc tcgatgagat gagggatagc cgtaaattga attaaaccag atagaaacaa
     5641 accggccgcc acttcattgg aagcgttgag aactgcgggg gcagttccgc ccgctcgagc
     5701 ggcctccatg cagaggcaaa atccaggaaa ctgctctgtg tccacagtgc gaaactcaag
     5761 tggcggccac gattctggcc tggtggcgat tccaagctca ggaaccggat ctggataata
     5821 cagagcatgc gcgatcggaa acaccatggt gggcctgctt gccatcattc ggtaaccatc
     5881 tggtgtctcc accatcgcat gcacatggct gaccggatga atcaagacgt cgagctgcga
     5941 gtatggaatt ccgaatagat gatgcgcctc tatgatctcg aggcccttgt tgatgagacc
     6001 ggcagaatct actgtaatct ttggccccat gctccaggta ggatgattta aaacttcatc
     6061 acgactcact ttctctacgt ccgctctact cttgctcaga aaaggaccgc cggatgcagt
     6121 cagaatcgcg cgacgaagat gctctggttc tactccaagc aacagctgaa atagggcatt
     6181 gtgttcggag tctacgggca cgatgcaagc acgcgaaaga gccagtgcgt tttcaatgac
     6241 cggtcctgca gtgacgagtg tttctttgtt tgcaagcgcg atcttgagcc cgatctgaat
     6301 ggcttcgagt gtgggcgcaa ttccagcaga acccatcact gcagtcaaaa cagtatctgc
     6361 tccggcctgt gcgcccaatc gtacgagttc tacgaggccg ctgcttcccg aaaagaactg
     6421 aacggaagga aacgacgcag aaagcctggc caatccctgg gccatttctg gatctgtgat
     6481 ggccgcataa cggatggttg gaaattttga aatgatctct gcaagtctgt ctgctttgcc
     6541 gtgaacgctc acggcaacga gttctattcc gctttctggt aaaatggaaa gagtgctact
     6601 tccgatcgac ccggatgcgc cgagcaagat gagctttcgc gtacgtttcg cacgggccgg
     6661 tgccgagatc tgcgccgaat attcaaagaa cgccatgctg gatcaaagag tcaggccagc
     6721 gatgccacga atgtacaaat agaagtaacc aatgggaatt gtgaaataga gagagtcccc
     6781 tcggtcgagc attccgccgt gacctggaat caaggcagct gaatctttct tttttgagtc
     6841 acgcttgatg gcactttcca ccagatcccc aaatacagaa atcacagaca gcaccgcagt
     6901 gaagagcgcc atttctacgt aacccatgtg gatttcgtaa gcaaatctct tccaacagta
     6961 aagaaacccg agcatcaaaa gaatggagaa aacaaaaccg ccaacgtaac cttcaatcgt
     7021 tttgcgcgga ctcaccttca gacctgcatt gtgttttcca aagtaacggc ccgcaaaata
     7081 cgcccctgtg tccgttccga tggttgccag agccactact acgaaaaaga acaaacccct
     7141 ttcctctgcg aggatgagaa acgcatggca tagggtcat
//
LOCUS       DAOQJL010000151         7159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5800_length_7159_cov_4.342765, whole genome shotgun sequence.
ACCESSION   DAOQJL010000151 DAOQJL010000000
VERSION     DAOQJL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_7159_cov_4.342765"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLG78_08360"
     CDS             complement(<1..878)
                     /locus_tag="PLG78_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23166.1"
                     /translation="MKRFQFKTYDEAVIAQVRQAVDSCLSQNGLEMSRHIIETVLSEL
                     IQNAVKANLKRVFFTSRQNNFKSDSHASEQFGEALAADAGALLSLLKNAPLSVRVEIF
                     AKQKVEIHVFNSTQMNASETKRVRQALQGKRVKGEGAGIGLGMCARLLDGIGIKKGLS
                     FQSNKNGTLFRLKIARGITAKDPRKSRVERFVQSCLPLPVAKTTADQIRGGAAPEALP
                     DAAMQFNLLHCGEKTPRTSRIKTTSNPASLEILGAMQAGEWEGIERYFLTTLSPGRLQ
                     ELRAISGRGKASRRAG"
     gene            complement(875..1591)
                     /locus_tag="PLG78_08365"
     CDS             complement(875..1591)
                     /locus_tag="PLG78_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE23167.1"
                     /translation="MLLAIPVYNEKKTIPSLIKQTLANLPEQVHRVVCIDDGSTDGSA
                     EVLDEEARRHSNIEIVHQAQNGGYGRSILTSLEIGRARGYTHVITMDCDLQHRPEDLI
                     RFVQEPLDIDLVSGSRYMPESQSQGHAPGDRMEINGRITALLNSRYNWNLTDSFCGFK
                     RFRLAKIDPSVFSETGYAFPMEFWAYARAQKLTIHEIPVSRIYTTDNRTFGEDLDRKR
                     RRYRYYLETLHRSERKFKLQ"
     gene            1590..2516
                     /locus_tag="PLG78_08370"
     CDS             1590..2516
                     /locus_tag="PLG78_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE23168.1"
                     /translation="MFRKTRFGIAISGGGCKAFFGLGVGAVLRDAGLNLVSYSGASAG
                     SAMAASLFARNYRDVVRYFRSVTLRNPSNFHFRNLFLGARPFPHERIYRRAVRTYMDW
                     RVFVESKERIAINALEVPHELYPTSFARARLLVRIVDAFRKEMKLYREGRYKSLLSEC
                     VRACGLVEKVFRNEDIDSPEHLEDIILASSSVPPMVKFQKVKGRYYLDGGITRNLPVA
                     LLENVDVAIGIYYNFWTRLQYEGVNVVDGGQEIEKMEKGKKAGPRVILVSPERDLPIQ
                     TWDYANPRGIELAYEAGLRAGEHLLPIVKKMR"
     gene            2614..5832
                     /locus_tag="PLG78_08375"
     CDS             2614..5832
                     /locus_tag="PLG78_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23169.1"
                     /translation="MSIASVSIKRPIFITSIVLVIIFTGAFSLLKLGVDLFPDVNFPF
                     VVVSTVYTGAGPEEVENLISRPLEEELSSLQGVKRITSRSQEGFAVVFVEFTLSTDIK
                     YGEQQVRNRVSRVRRLLPSSIEEPIIVRFDPADQPVVRYALFADLPPARLYELAKEKI
                     KPRLEQAPDVGSIALIGGVKREIQIEIDRNKLNALQLSAVGIGQSMKNYGLNVPVGKH
                     EQGASETTFRTIGQFDSLKQIEEVGISFGGEVGNAVSLRSVATVRDGTEDAKNICELW
                     APTTTQPEYGFFSRNRHAQIERQNNAALFIDIYKQSGSNTVTVTDGVKLRVEKLNEFL
                     KDQEGHPRLVMVRDGSRWIRANVEDVGLAIVLGILLTVLVVYLFLGNIRSTIITGLAL
                     PNSLLGAFILMYIMGFTINVMTLLALSLAIGLLVDDAIVVRENIFRKMELGESPVKAA
                     EDGTTEVALAVIATTATVLAVFLPIGFLSGIVGQFFKQFGLTVCFAMIISLFDAMTIA
                     PMLSAYFGGQVHGEPNFVVRGFRRFQDFLEKGYIAIMRFGLKRPIVIILLTVLVFVGS
                     ILTLGGVKKTFLPPNDQGEFMVSIQLPPGTALQGTYDAALKIREKLQKIPEMNLIAMV
                     VGSTEGEPNVAIFGVGLTDFTQRTRSTQKVQEEIREMLKPYAEWKPSVNEYSAVGGGV
                     QYPFNVNIMGDDLQQLQPYAEQVVTMLKGIPDLTDVSMDYRAGKPEYRIEFDPNRMQN
                     VGVNPTVAGAELRYHIAGEVVGTLRERGYEYDIRMRLRPEQRNLKSAYAQTRVPNNQF
                     KMIPLAAIATGRPALGPEKITRQDRSRVIQIHANLAPGGAIGSATDIAKKALTTKLIP
                     PKGTSYVFWGQSEDFIELLVNVGIAFLFAIVFIYLVLASLYESFITPLAILISIPPAI
                     SGAFLSLYIFNEMLNIFSMIGLILLMGLVTKNSILLVDYALEGIRGGLDINEAILRAG
                     RTRLRPILMTSLAMIAGTMPIALGLGEASRARTAMGIAIIGGIVVSTVLTLIVVPAIF
                     GYIERFREWIEAKFRPAEIEAIRLQELSSMSQAMREPEKKRK"
     gene            complement(5925..6749)
                     /locus_tag="PLG78_08380"
     CDS             complement(5925..6749)
                     /locus_tag="PLG78_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23170.1"
                     /translation="MSIGNLPVLAHDMVLMIKAEVKRDLLYMKRYPLEIISFMFFMFL
                     ILIAIIYGIDFLLPKGSGISDKGNKEKLVLGYCLMQFVMATQMGWSGQIQNESQTGTL
                     EQLSISGHSLGGVLFARGIAQLPRQTITFYIMLALYRIALPGSAISLNHMLAGSLDLL
                     IMAIGIFGISYIFAGITLLFKRVGFFFQIINFAFLGLFWIDRAKLPEILQNIYDHFPL
                     TVGMSRLMQTLTDPVIASPYSMIEFIVYSFLFMAVGWFCFSRMERTARSYGLLSQY"
     gene            complement(6746..>7159)
                     /locus_tag="PLG78_08385"
     CDS             complement(6746..>7159)
                     /locus_tag="PLG78_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23171.1"
                     /translation="EMMLELTRKHGKSILLTTHQMDLVEEVADRAGIIREGKIVREGT
                     IPELRNVFRNHIYRFSVRGKFKLPPEWQKKYGAALLSGQNDHTEWEMDCKKPEGLYTV
                     LSALGKKKLEIISMHKPVEDLEEIFMRTLSEEATR"
BASE COUNT         1686 a         1728 c         1842 g         1903 t
ORIGIN      
        1 attccggccc gcctggacgc tttgcctctt ccgctgatcg ccctgagttc ttgcaaccga
       61 ccgggagaca gagtggtcaa aaagtaccgt tcaattcctt cccattctcc ggcttgcatg
      121 gctcccagaa tttcaagcga cgcaggattt gaagtagttt tgatcctgga agtgcgaggg
      181 gttttttctc cgcagtgaag caggttgaat tgcatggccg catccggtag agcttccggc
      241 gccgctccac cgcgaatttg atctgccgtg gttttcgcga ctggcaacgg caaacaggat
      301 tgcacaaatc tttctaccct ggactttcgc gggtcttttg ccgtgattcc gcgcgcgatt
      361 ttcaatcgaa agagagttcc attcttattc gactgaaagc tcagcccttt tttgattcca
      421 atgccatcga gcaggcgagc gcacattcca agaccgatcc ccgcgccttc gccttttact
      481 ctttttccct gcaatgcctg acgcactctc tttgtctcgg aggcgttcat ctgcgtgctg
      541 ttaaaaacat ggatctccac tttttgcttt gcgaaaatct cgactcgcac gctgagaggt
      601 gcgttcttca gaagactcag tagagctccg gcgtccgctg ccagagcttc gccaaattgc
      661 tctgaagcgt gcgaatcgct tttgaagttg ttttgcctgg aagtaaagaa gactcttttt
      721 agattcgctt tcactgcgtt ctggatgagt tcgctcaata ctgtttctat aatgtgcctg
      781 gacatctcga ggccgttctg gctgaggcac gaatccactg cctggcgcac ctgtgcgatg
      841 actgcttcat cgtatgtttt gaattggaat cgtttcactg tagtttgaac tttctttcag
      901 agcgatgcag cgtctctaga tagtatcggt atcttctcct cttgcgatcg aggtcctccc
      961 cgaaagttct gttgtctgta gtatagattc tgctcacagg aatctcgtgg atggtcagct
     1021 tctgcgcccg cgcgtaggcc cagaactcca tggggaaggc gtaaccggtc tccgaaaaaa
     1081 cagaaggatc gatcttcgcg aggcgaaatc gcttgaaacc gcagaacgaa tctgtgagat
     1141 tccagttgta gcgcgagttg agcaatgcag tgatgcgccc gttgatttcc attcgatcgc
     1201 ccggagcatg tccctgagat tgcgattcgg gcatatatct ggagccgctt accaggtcga
     1261 tgtccagggg ttcttgcaca aatcgaatca aatcttccgg tctgtgttgc agatcgcagt
     1321 ccattgtgat gacgtgagtg taacctcggg ctcgtccaat ctcgagcgat gtcagaatgc
     1381 ttctgccgta tcctccattc tgagcctggt ggacgatttc tatattgctg tggcgtcgcg
     1441 cttcttcatc gagaacttcc gccgatccgt ccgtggagcc gtcgtcaatg cacacaaccc
     1501 ggtggacttg ctcaggcagg ttggcaagag tctgcttgat caaagatgga attgtttttt
     1561 tttcgttata gaccgggatg gctagaagca tgtttcgaaa gacgcgattt gggattgcca
     1621 tttccggcgg tgggtgcaag gcattttttg ggctcggagt gggtgcggtt ctacgagatg
     1681 ctggattgaa tctggtttca tatagcggcg caagtgcagg atccgccatg gccgcgagtt
     1741 tgtttgcacg caactatcgc gatgtagttc gctacttcag atctgtcact cttcgcaatc
     1801 cctctaattt tcattttcgt aatcttttcc ttggcgcgcg tcctttccct cacgaacgaa
     1861 tctacaggag agctgtgcgg acctacatgg actggcgcgt ctttgttgaa tcaaaagaac
     1921 gcattgccat caatgcactg gaagtgccac atgaactcta tccaacatcc tttgcacggg
     1981 cccgcttgct ggtccgaatc gtggatgcat ttagaaaaga aatgaaactt tatcgcgaag
     2041 ggcgatacaa gtctttgctt tcagaatgcg ttcgtgcatg cggcctggtg gagaaagtct
     2101 ttagaaatga agacattgat tcgccagagc atctagaaga catcatcctt gcatcgagca
     2161 gcgtgccgcc gatggtgaaa tttcagaagg tgaaaggccg ctactatcta gatgggggaa
     2221 ttacacgcaa tttgcctgtt gcattgctcg aaaacgtgga tgtggcgatt ggcatttatt
     2281 acaacttctg gacccgcctg caatacgaag gagtcaacgt tgtggacggc ggccaggaaa
     2341 tagagaagat ggaaaagggc aagaaggccg gtccgagagt gatccttgta tccccggagc
     2401 gcgacctccc tatccagacc tgggactatg cgaacccccg cggaattgag ctggcgtatg
     2461 aggccggact gagggccggc gagcatcttt tgccgatcgt taaaaaaatg cgctgagcaa
     2521 agcccgcgcc gttctgcttc aaaatcctgt ctctgccgcg atcattcgga aaaaaaaaca
     2581 gacagcctgg tccatttccc gagactcgcc ctcatgtcga tcgcaagtgt ctcgatcaaa
     2641 cgccccattt ttatcaccag tattgttctg gtcatcatct tcacaggtgc attctcctta
     2701 ttgaagttag gcgtagattt gttcccggat gtgaacttcc cgtttgtggt tgtgagcacc
     2761 gtgtacacag gcgccggtcc ggaagaagta gagaatctga tctctcgccc tctggaagaa
     2821 gaactcagtt cccttcaggg tgtgaagcga atcacttcgc gcagccagga aggcttcgca
     2881 gttgtgtttg tagagttcac tctcagtaca gacatcaagt atggagagca gcaggttcga
     2941 aatcgagtct cgcgcgtgcg acgccttttg ccaagttcca ttgaagagcc aatcattgtg
     3001 cgatttgatc cggcagatca gcctgtggtc cgttatgcgc tcttcgcaga tcttcctcca
     3061 gctcgccttt atgaactggc aaaagaaaaa atcaagccaa ggctggagca ggcgccggac
     3121 gtgggatcaa tcgcactcat tggcggagtg aagcgcgaaa ttcaaattga aattgatcgc
     3181 aacaaactca acgcactgca gctttccgca gttggaatcg ggcaatccat gaagaattat
     3241 ggcctgaatg ttcccgtggg taaacatgaa cagggtgcat ctgaaaccac attcaggacg
     3301 atcggtcagt ttgattcgct taaacagatt gaagaggtgg gcatttcatt tggtggagaa
     3361 gtgggcaatg ctgtcagtct ccgaagcgtg gcaactgtaa gagatggaac agaagatgcg
     3421 aagaatattt gcgaactctg ggcgcctacc accacacagc cagagtacgg attcttttcg
     3481 cgcaatcgac atgcgcagat agaacgccag aacaatgctg ctctattcat tgatatttat
     3541 aaacaatccg gttccaacac tgttacagtt acggatggcg tgaaactccg ggtggagaag
     3601 ctcaacgaat ttctaaaaga tcaggaaggg catccaagac tcgtcatggt tcgtgacggt
     3661 tcgcgctgga tccgcgcaaa cgtggaagac gtgggcctgg cgattgtgct cgggattctt
     3721 ttgactgtgc tcgttgttta tctgttcctg ggtaacattc gttctacgat cattaccggt
     3781 ctggcacttc caaacagttt gctcggtgca ttcattttga tgtacatcat gggctttacc
     3841 ataaacgtga tgacccttct tgctctgtct cttgcgattg gtctgctcgt agatgatgcg
     3901 atcgtagtgc gtgaaaacat tttcagaaag atggagctcg gagaatctcc ggtcaaggcc
     3961 gcggaagatg gaacgactga ggtggctctt gctgtgatcg cgactacggc cacagtcctg
     4021 gcggtgttct tgccgatcgg ctttctctcc ggcatcgttg gtcaattttt taaacaattt
     4081 ggtctgactg tttgttttgc gatgatcatt tctctctttg atgcgatgac cattgcgccc
     4141 atgctttctg cttattttgg cggccaggtt catggagagc caaatttcgt tgtgagaggt
     4201 ttccggcgtt ttcaggactt tcttgagaaa gggtacattg caatcatgcg atttgggctc
     4261 aagcgtccca tcgtcattat tctgctcact gtgctcgttt tcgttggaag tattttgact
     4321 ctgggtggcg taaaaaagac cttccttccg cccaatgatc agggcgaatt catggtctct
     4381 atccaacttc ctccgggcac agcactgcag ggaacttatg atgctgctct caagattcgc
     4441 gaaaagcttc agaagattcc tgaaatgaat ctcattgcaa tggtcgtcgg aagtacggaa
     4501 ggggagccaa acgttgcgat ctttggcgta ggtttgaccg actttacgca acgcactcga
     4561 agcacgcaaa aggttcagga agaaattcgc gaaatgctca aaccatatgc tgaatggaaa
     4621 ccttccgtga atgaatacag tgctgttggc ggcggcgttc agtatccttt caacgtaaac
     4681 atcatgggag acgacttgca acaactgcag ccttacgcag agcaggtagt gaccatgctc
     4741 aaaggaattc cggatcttac ggacgtctcc atggactatc gggccggtaa accggaatat
     4801 cgcattgagt ttgatccaaa ccgcatgcaa aacgtgggag tcaacccgac tgtggccggg
     4861 gcagagcttc gctatcacat cgcaggtgag gtagtgggaa cgctgcgcga acgcggatat
     4921 gaatatgaca ttcgcatgcg ccttcgtccg gagcaaagaa acttgaaatc cgcttacgcg
     4981 caaacgcgtg ttcctaacaa tcaattcaag atgattcctc tggcggccat cgccacagga
     5041 aggccggccc ttggtccaga aaagatcacg cgtcaggata gatctcgcgt gattcagatt
     5101 catgcaaacc tggctccggg aggtgcgatt ggatctgcga cagacattgc aaaaaaagcg
     5161 ctcactacaa aactgattcc gcccaaagga accagctacg ttttctgggg ccaatcagag
     5221 gacttcattg aactcctggt gaatgtgggc atcgctttct tatttgcgat tgtattcatt
     5281 tatctggttc ttgcgagttt gtatgaatcg ttcattactc ctcttgcgat tttgatttcg
     5341 attccacctg cgatttctgg tgcgttcctt tccctctaca tcttcaatga gatgctcaac
     5401 atcttctcga tgattggttt gattctattg atgggccttg tgaccaagaa ctcgattctg
     5461 ctggtggact acgctcttga aggaatcagg ggtggcctgg acatcaatga agcgattctc
     5521 agagcgggtc gaaccagact tcgacccatc ttgatgacga gccttgcaat gatcgcaggc
     5581 accatgccga ttgcgctcgg tctcggggaa gcgtctagag ctcgcactgc gatggggatt
     5641 gccatcatcg gtggtattgt tgtttccaca gtgctgactc tcattgtggt tcctgcaatc
     5701 ttcggataca ttgagagatt ccgcgaatgg atcgaagcga aattccgccc cgcggaaatt
     5761 gaagcaattc gacttcagga actcagctct atgtcccagg caatgcgcga gcctgagaag
     5821 aaacgcaagt aagccttcgc gaagctcacg aagaattgcg ggcaggtgcg tccggatttc
     5881 cgggcgcgac ctgtgatgtg ggcgtttctt actctgagat tggatcagta ctgagagagt
     5941 agtccgtaac tgcgtgccgt tctttccatg cgagaaaagc aaaaccagcc cactgccata
     6001 aacaagaacg aatacactat gaattcaatc atagagtagg gggacgcgat gactggatcg
     6061 gtgagtgttt gcatgagcct ggacattcca actgtgaggg gaaagtgatc gtaaatgttc
     6121 tggagaattt caggaagctt ggcccggtct atccagaaca gacccagaaa agcaaaattg
     6181 atgatctgaa agaagaaccc gactctttta aaaagtagag tgatcccggc aaatatataa
     6241 gaaatgccaa atataccgat ggccatgatg agaagatcca gagaaccagc cagcatgtgg
     6301 ttcaaggata tagcgcttcc aggaagagca atgcgataaa gagcaagcat gatgtagaac
     6361 gtaatggtct gtcttggcaa ttgtgcgatt ccgcgcgcga atagaactcc gcccagagaa
     6421 tgacctgata tagaaagctg ttcgagcgtg ccagtctggc tttcgttttg aatctggccg
     6481 gaccatccca tttgagtggc cattacgaat tgcatgaggc aataacccag tacgagcttt
     6541 tctttgttcc ctttgtcgct gatgccggat ccctttggca aaagaaaatc gattccataa
     6601 atgatcgcga tgaggatcaa aaacataaag aacataaacg atatgatctc aaggggatat
     6661 cgcttcatgt agagcaggtc tcttttgacc tccgccttga tcatcaagac catgtcgtgt
     6721 gccaatacgg ggagattgcc aatgctcatc tggttgcctc ttcgctcagt gtgcgcataa
     6781 aaatttcttc caggtcttct accggcttgt gcatcgaaat gatttcgagt ttcttttttc
     6841 caagcgcgga cagaactgtg tagagacctt ccggcttctt gcagtccatt tcccattcag
     6901 tgtgatcgtt ctggccggac aggagtgctg cgccgtactt cttctgccat tcaggcggca
     6961 gtttgaattt tcctcgaacg ctgaatctgt agatgtgatt gcgaaataca tttctaagtt
     7021 cagggatggt tccttctctt acaatcttgc cttcgcgaat gatcccggca cggtctgcca
     7081 cttcttctac tagatccatc tggtgtgtgg tcagtagaat ggacttgccg tgctttcgcg
     7141 taagttctag catcatttc
//
LOCUS       DAOQJL010000152         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5894_length_7066_cov_3.986735, whole genome shotgun sequence.
ACCESSION   DAOQJL010000152 DAOQJL010000000
VERSION     DAOQJL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5894_length_7066_cov_3.986735"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLG78_08390"
     CDS             <1..273
                     /locus_tag="PLG78_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23172.1"
                     /translation="SVDTVKSGEYAPLSRPLFIYVSTKAAAKEEVKAYIDFYLTNGAK
                     LSAQVGYIALPDGVYTKAKSRFASLQTGSVFQMEGHEGKTIEQVFN"
     gene            323..1273
                     /gene="pstC"
                     /locus_tag="PLG78_08395"
     CDS             323..1273
                     /gene="pstC"
                     /locus_tag="PLG78_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNE23173.1"
                     /translation="MSDLLTQAVRGNRRDVFSRYGERPSYGVKERFVRYTLRTFSYFT
                     VFVTLAIVFILVIDASEFFSQASFLEFITGREWLPFGEPKKLGVLPLLTGTVMIAVGS
                     SLVSIPIGLGIAIFLTQYTSRRFQRFMGPVLEVLGGIPTVVYGFFALTTLTPLLKHLF
                     TSIDTFNALSAAIVVGLATLPMVASLSADALQVVPSSIRNAGYALGMSKFHVVTRIMV
                     PAAFSGIVASFLLAFARAVGETMAVSIAAGSTPSMSFDYLKSIQTMTAFIVQISMGDT
                     AAGSIEYYTIYAIGISLFVLTFAFNFAATAIIRRFREVYQ"
     gene            1270..2133
                     /gene="pstA"
                     /locus_tag="PLG78_08400"
     CDS             1270..2133
                     /gene="pstA"
                     /locus_tag="PLG78_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE23174.1"
                     /translation="MKDLRVRYRIYGAIFKGFCFSANVLAFGFLLYLLGSVLFQGVPN
                     INWTFLTEYPSRFPQKAGILAAIAGTVWMMVLTAFVSIPLGIGAAIYLEEYARPGRLV
                     NLVRLNIQNLAGVPSIIYGMLGLTMIVRGLGLGRSILAGALTMALLILPVITIATQEA
                     LRAVPGSLRLAGFGIGMTRWQVVRYQVFPSALPGIMTGIILALSRAVGESAPLLLVGA
                     TAYSAYLPSSPMDGFTVLSVQIYNWIDQPQKEFQALAAGAIVILLVLLIALNSIAIYL
                     RQYYRKKVSTG"
     gene            2138..2941
                     /gene="pstB"
                     /locus_tag="PLG78_08405"
     CDS             2138..2941
                     /gene="pstB"
                     /locus_tag="PLG78_08405"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE23175.1"
                     /translation="MQQITQKIAAPENGDATRTEAVFACSNVNIHYGDFHAIQDLSLD
                     LLENRVTALIGPSGCGKSTFLRSLNRMNDFIDSFRISGSLLLDGEDIYAPDVDPVEIR
                     LRVGMVFQSPNPFPKSIRENVALGPRINGYKGNLAELVEEALVKSYLWDEVKDKLDQS
                     AYSLSGGQQQRLCIARAIAMQPEVLLMDEPTASLDPISTKRIEELIVELKKSYSIVIV
                     THNMQQAARIGDFTAFFYQGKLIEYDSTFRMFTNPGQKLTEDYITGRFG"
     gene            complement(2942..4840)
                     /locus_tag="PLG78_08410"
     CDS             complement(2942..4840)
                     /locus_tag="PLG78_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23176.1"
                     /translation="MRAWLSIFGLLLAASAIHGDAVTVPRTGFAEVEAGKVFILSDPG
                     VDSVEEITKREKDFKPATTLDLLDPGKVYWAYFDIVNPDPGMPVLEARSDLLHRIDLY
                     FEQDGRMVQKSAGISLPFMQREIAYRYSAFRLPMKPTIRVYLRLEFQIRQHMSLKLWN
                     EKWFTQKNRREQLILGVYYGIVIAMAVYNLFVFLSLRSVSYMHYVLYIISFALYQTTL
                     NGISFEYFWPQTAGLDIKASFVSAGLSIMFAALFARHFLGVEKRSRFFNFAFLGSAAL
                     AGLWAAASLILPFQTANVMGRVLGSLVVPLIIAGSIWALRDGYKPARYFLIAWTFLLI
                     GVLMFSLTGLGVLPWNPATRYSMQLGSVVEILLLSLALGDRINSLREQKDAAVTRTRE
                     LTAELQIAKRIQSSILPESNPQIEGLRIESAYRPASEVGGDFYDFHTEGKMVGLIVAD
                     VCGHGVGAALIASMVKISFANLRSHWKEPDEILRGLHRSMSPRIRGQFITACYAFVDM
                     NMRRLYFSSAGHPPVLIWRRREKRLHELGMRGPVIGWPGDWTCPSVSWDLEPGDRLVL
                     YTDGIFEQRNQKGEMFGFDRFYESISATSELTTKDAADAILSAVDSWHKKEDQLEDDV
                     TLILLDIE"
     gene            complement(4856..6010)
                     /locus_tag="PLG78_08415"
     CDS             complement(4856..6010)
                     /locus_tag="PLG78_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE23177.1"
                     /translation="MILTRPIILLSLVSLFTDIASEMLYPVMPIYLGSIGFSVFYIGV
                     LEGFAEATAGLSKGYFGNLSDAIGRRLPFVRGGYALSAISKPMMAVSVFPGWIFLSRT
                     VDRLGKGIRTAARDAMLSDASAPEHRGRVFGFHRALDTTGALLGPVLSLTFLYYLPGQ
                     YRTLFILAFIPGAFAILLTLFLSEIRGNSRTRPGILDFLKYAIHGPRSYRKLIAGLLF
                     FALMNSSDAFLILLMKQRGVSDSAAILAYIFYNTVFALAAYPAGMAADRFGAKRMLTI
                     GLLLFAFVYAAMPYAHSPITFALLLAMYGIYAAATEGIGKAWITTTAAPEDVATAVGT
                     FASLQSICTICASAAAGALWLWAGPEIVFGISAAGTFIAAMYFVFAFRTA"
     gene            complement(6007..6846)
                     /locus_tag="PLG78_08420"
     CDS             complement(6007..6846)
                     /locus_tag="PLG78_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE23178.1"
                     /translation="MQMRILATFLLIFMIQCSQKKNAEFCSVGGEELSPIVDSWSAIY
                     QESGRKVPQHEARGNRTAAPALASRICNLAFVSDALPSEDLDLITRKLGRTPGAIPIA
                     VEIIAFAVPKSFPVDSLSVEQIKSIFGSGEEDASALGLPPGKISAFGINSASDRYFWL
                     KGALGIRAFSDRVKEMSGPLTLMDRVAVTSYGIGYGRPLEANGVKLLDISKDGRVLGI
                     KSTDYPFRRYYFAYVTEKTPDTKAFLEFVLSDRGQKALLPSGLYPLPDSVLIKAREEL
                     ARQ"
     gene            complement(6894..>7066)
                     /locus_tag="PLG78_08425"
     CDS             complement(6894..>7066)
                     /locus_tag="PLG78_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE23179.1"
                     /translation="ERFFRADQNRSRREGGSGLGLSIVRQIARIHGGNVTVQDGPQGG
                     SVFRVLLPQTPD"
BASE COUNT         1714 a         1769 c         1790 g         1793 t
ORIGIN      
        1 tctgttgaca cagtcaagag cggcgaatat gctcctcttt cgcgtccact gttcatctac
       61 gtgagcacaa aagcggccgc aaaagaagaa gtgaaggctt acattgattt ctatctcacc
      121 aatggagcca aactttccgc tcaggtgggc tacattgcac tgccggatgg agtctataca
      181 aaagcaaaat ctcgtttcgc gagtctccag actggatctg tttttcagat ggaaggccac
      241 gaaggcaaga cgatcgagca agtttttaat tgaacaatcg caggcggacc cgtttctggg
      301 tccgctcgca ctttattgaa ccatgagtga tttacttacc caggccgttc gcggcaatcg
      361 cagagatgtt ttttcccgtt atggggagag gccttcttac ggggtaaagg aacgattcgt
      421 tcgctatact cttcgcacct tctcgtactt cacagtcttt gttactctcg caattgtatt
      481 catactcgtc atcgacgcat ccgagttttt ttcccaggca agctttctag aattcatcac
      541 gggtagagag tggctgccat ttggagagcc aaagaaactc ggcgtgcttc ctttgcttac
      601 tggcactgtg atgattgctg ttggatcctc gctggtttcc attcccattg gacttggaat
      661 cgccatcttt ctcacgcagt atacatcgcg caggtttcag cgtttcatgg ggcctgttct
      721 tgaggttctc ggtggaattc ctacagtggt atatggtttc ttcgcgctca ccactcttac
      781 tcctcttctc aaacatctat tcacaagcat tgatacattc aacgcactgt ctgctgccat
      841 tgtggtgggg ctggcaactc ttcccatggt tgcatctctc tctgcggacg cgctgcaggt
      901 ggtcccttct tcgattcgca atgcaggata tgcgctgggt atgtccaagt ttcatgtggt
      961 gactcgaatc atggtgcccg ccgcgttttc tggaattgtt gcctctttct tactcgcgtt
     1021 tgctcgagct gtgggagaaa ctatggcggt gagcattgct gctggttcca cgcccagcat
     1081 gtcttttgat tatctcaaga gcatccagac catgaccgca ttcattgttc aaattagcat
     1141 gggagatacg gctgccggta gtatagaata ctatacaatc tatgcgattg gcatttcgct
     1201 ctttgtcctc acgtttgcat ttaactttgc cgcaacagcc atcatccgca gatttcggga
     1261 agtgtaccaa tgaaagatct gcgcgtgcga tatcgaatct atggagccat tttcaaagga
     1321 ttttgttttt ccgcgaacgt gctggcgttt ggctttctgc tgtacttgct cggctctgtt
     1381 ctcttccagg gcgttccgaa catcaactgg actttcttaa ctgaataccc ttccaggttt
     1441 ccgcaaaaag cagggatcct cgcggcgatc gcaggcactg tctggatgat ggtgctgact
     1501 gcattcgtga gcatcccgct tggaattggc gcggctatct accttgagga gtatgcacgg
     1561 cccggtagac ttgtgaatct ggttcgcctc aacatccaga atcttgcggg cgttccatct
     1621 atcatttacg gtatgcttgg cttgaccatg atcgttcgcg gtctgggtct gggcagaagc
     1681 attcttgcgg gtgccctcac catggcgctt ctgattcttc cggtcattac catcgcaacg
     1741 caggaagctc ttcgcgcagt ccctggttca cttcggttgg ctggattcgg gattggaatg
     1801 actcgctggc aagtagtgcg ctaccaggtg tttccttctg cgctcccagg aattatgacc
     1861 ggaatcattc ttgccctgtc gcgcgcagtg ggggaatcag ccccgctctt gctcgtcggt
     1921 gcgaccgcgt attctgccta tcttccatcg agtcctatgg atgggtttac ggttctatcg
     1981 gttcagattt acaactggat tgatcagcct caaaaagaat tccaggccct tgcggccggt
     2041 gccattgtta tattactcgt tttactcatt gctctcaatt caattgcaat ctatctgcga
     2101 caatactatc gcaaaaaagt gagcacagga taatcgtatg cagcagatca ctcagaaaat
     2161 tgcggcacct gaaaatggcg acgcgactcg cacagaagcg gtgtttgcgt gtagcaatgt
     2221 gaacattcac tacggtgatt ttcatgcgat ccaggatctt tctctggacc tccttgagaa
     2281 tcgagtgaca gcattgattg gaccatcggg ttgcggaaaa agtacgtttc ttcgcagtct
     2341 aaatcgcatg aacgatttca tcgattcgtt tcgtatttca gggagtcttt tgttagacgg
     2401 cgaagacatc tatgcacctg acgtggaccc tgtggaaatt cgtttgagag ttggaatggt
     2461 atttcagagt ccgaatccat ttccaaaatc gattcgcgaa aacgtggctc ttggcccgcg
     2521 catcaatggt tacaagggaa acctggccga acttgtggaa gaagcgctgg tcaaatccta
     2581 cctctgggat gaagtaaaag acaagctcga tcaaagcgcc tactcgttgt caggtggtca
     2641 acagcaaagg ctatgcattg cacgcgccat cgccatgcag ccagaagttt tgctaatgga
     2701 tgagcctacg gcttctctcg atccaatttc aaccaagagg attgaagaac tgatcgtaga
     2761 gctcaagaaa agctattcta ttgtaattgt gactcataac atgcagcagg cggcccgcat
     2821 cggcgatttt actgcgttct tctatcaggg taagttgata gagtacgatt ccacctttcg
     2881 catgtttaca aatccaggtc agaaactcac ggaagactac atcacaggca gatttggctg
     2941 atcactcaat gtccagaagg atgagagtga cgtcatcttc cagctgatct tctttcttgt
     3001 gccaactatc cactgctgat agaatcgcat ccgctgcgtc tttcgtcgtg agttctgacg
     3061 tggcggaaat ggattcatag aagcgatcaa acccgaacat ttcccctttc tgatttcgct
     3121 gctcaaagat tccatctgtg taaagcacca gacgatctcc tggttccagg tcccagctta
     3181 cagaagggca agtccaatct cctggccaac caatgacagg gccgcgcatt cccagttcgt
     3241 gaagacgctt ttctcttctt ctccagatta gaacgggtgg gtggcctgcg gaagaaaagt
     3301 aaagcctgcg catattcata tctacaaacg cgtagcatgc tgtgatgaac tggccgcgaa
     3361 tgcggggaga catggaacga tgaagccctc gcagaatttc gtcaggttct ttccagtgcg
     3421 aacgaaggtt cgcaaatgaa atttttacca tagacgcaat gagcgcggca cccacaccgt
     3481 gcccgcatac gtctgcgacg atcagtccca ccatttttcc ttctgtatga aaatcgtaga
     3541 agtctcctcc tacttcgctc gcagggcgat aggcgctttc gattctcagt ccctcgattt
     3601 gcggattcga ttctggaagg atggagcttt gaattctttt tgcaatttgt agttccgctg
     3661 tgagctctcg agtgcgcgtc acggccgcgt ctttttgttc tcgcagcgag ttgattctat
     3721 ctccaagtgc aagagagaga agcaggattt ccactaccga tccaagttgc atactatatc
     3781 ttgtggccgg gttccatgga aggacaccga gtcctgtcaa agagaacatc aaaacgccaa
     3841 tgagaaggaa ggtccaggcg atcaaaaagt atcgggccgg cttgtatcca tcgcgcagcg
     3901 cccagatgga acctgcgatg ataagaggaa ctaccaggct tccgaggact cttcccatca
     3961 cattggcagt ctggaaagga aggatcaaac tggcggcggc ccagagccct gccaatgcgg
     4021 cggaacccag aaatgcaaaa ttgaaaaagc gagatctttt ttctacacca agaaaatgtc
     4081 gcgcgaagag tgctgcgaac atgatggaga gaccggcgga aacgaaggag gctttgatgt
     4141 cgagtcctgc agtttgtggc cagaagtact cgaatgatat gccgtttaac gttgtctgat
     4201 acagtgcgaa tgaaatgata tagagcacat agtgcatgta actcacagac cgcagtgaga
     4261 gaaatacaaa gaggttatac actgccatgg caatgacaat gccatagtaa acacccagga
     4321 tgagctgttc gcgcctgttc ttctgggtga accacttttc attccacagt ttcaaagaca
     4381 tgtgctgccg aatctggaat tctaatctga ggtacacgcg aattgttggt ttcatcggga
     4441 gtctaaatgc agaataacga taggcgattt ccctctgcat gaatgggagg ctgatcccgg
     4501 ccgatttctg aaccattcgt ccgtcctgtt cgaagtacag atcaatacga tgcagcagat
     4561 cagatcttgc ctccagcacg ggcattcccg ggtccgggtt tacaatatca aagtaggccc
     4621 agtatacttt ccccggatcg agcaggtcca gagttgtggc cggtttgaag tctttctctc
     4681 ttttcgtgat ttcttccacg gagtccacac ctggatcgga gagaatgaat actttccccg
     4741 cctccacctc agcaaatcca gtgcgcggaa ccgtcacagc atctccgtga atggcggacg
     4801 cggccaatag taacccaaaa atggaaagcc aggcgcgcat taagacccaa atggatcaag
     4861 cagtacggaa tgcaaacacg aagtacatgg cggctataaa agtgcctgcc gcggatatac
     4921 caaatacgat ctccggcccg gcccagagcc acagagcgcc ggccgcggcg cttgcgcaaa
     4981 ttgtgcagat actctgcaaa gaggcaaacg tgcctacagc cgtggccaca tcttcgggtg
     5041 ccgctgtagt cgtgatccag gctttcccaa ttccttctgt cgcggcggcg tagataccgt
     5101 acattgcgag taacaacgca aaggtgattg gtgagtgcgc atagggcatg gccgcgtaaa
     5161 caaatgcaaa aagaaggagt cctatcgtca gcattcgctt tgcgccaaag cgatcagctg
     5221 ccatccctgc tggataggcg gccagggcaa atacagtatt gtagaaaata taggcaagta
     5281 tagcggcaga atcgctcacc cctctttgct tcatgaggag aatgagaaat gcgtccgaac
     5341 tattcatcaa tgcgaagaac agtagccctg cgatgagctt gcgataggat cggggaccgt
     5401 ggatggcgta tttcaaaaaa tccaggatcc ctggacgcgt tcgagagttt ccgcggattt
     5461 ccgaaaggaa cagagtaagg agaatggcga aggctccagg gatgaaggca agaatgaaga
     5521 gcgtccgata ctgtcctggt aaatagtaaa gaaatgtaag tgatagcacg ggaccgagca
     5581 aggctcctgt cgtatccagg gctctgtgaa acccgaacac tcttcctctg tgttctgggg
     5641 cagatgcatc cgaaagcatc gcatctctgg cggcagtgcg aattcctttt ccgagtctgt
     5701 ctacggtgcg tgataaaaag atccagcctg gaaaaacaga aacagccatc attggtttgg
     5761 aaatcgcgct gagcgcgtat cctcctctga caaacgggag tctacggcca atggcatctg
     5821 atagattgcc aaagtatcct ttggatagtc ctgccgttgc ttctgcgaat ccctcgagca
     5881 ctccaatgta gaatacggaa aaaccaatcg atcccagata gatgggcatg accggataga
     5941 gcatttcgct tgcgatgtcc gtaaaaagac tcacaagcga aagaaggatg atggggcgcg
     6001 tgaggatcac tgacgcgcca gttcttccct cgctttgatg agaacggaat cgggaagagg
     6061 atagagtccc gaggggagga gcgctttttg tccacgatcg gatagaacga attccagaaa
     6121 cgcttttgtg tccggagtct tctctgttac gtacgcgaaa tagtatctgc gaaaaggata
     6181 gtccgttgat ttgattccca gcactcgccc gtctttgctg atgtccagta gcttcactcc
     6241 atttgcttcc agaggccttc cataaccaat gccgtatgac gtgaccgcaa ctcgatccat
     6301 cagcgtcagt ggccctgaca tttcttttac tctatcagaa aaagcacgaa tccccagggc
     6361 acctttgagc cagaagtacc tgtctgaagc tgagttgatt ccgaacgcag aaatttttcc
     6421 gggcggaagg ccaagtgcgc tcgcatcctc ttcgcctgat ccgaaaatcg atttgatctg
     6481 ttctacgctt agagagtcca ccggaaagga tttgggcact gcaaaagcga tgatctccac
     6541 tgcgattgga atggcgcccg gtgtgcgacc gagcttccgc gtgattagat ccaggtcctc
     6601 tgaagggagt gcgtcggaca caaaggcaag attgcatatg cgcgaagcaa gggcaggagc
     6661 ggccgttcta tttccgcgcg cctcgtgctg tggaactttt cttcctgatt cttgatagat
     6721 ggcagaccag gagtctacga taggactgag ttcttctcca cccacactgc agaattcagc
     6781 gttctttttc tgggaacact gaatcatgaa gatcagaagg aaggtggcca ggattcgcat
     6841 ttgcatcgag tagatccagt ggtgccggcc gtccgggtca agccgaaagc atttcaatca
     6901 ggcgtttgcg gcaacagaac acgaaacaca gatccgccct gagggccatc ctgcactgta
     6961 acatttccgc cgtgaatgcg agcaatttgt ctgacaatgc tcaggccgag tccggatcca
     7021 ccttctctgc gcgatctgtt ctggtctgcg cgaaagaatc gttcaa
//
LOCUS       DAOQJL010000153         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5899_length_7063_cov_5.106735, whole genome shotgun sequence.
ACCESSION   DAOQJL010000153 DAOQJL010000000
VERSION     DAOQJL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_7063_cov_5.106735"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLG78_08430"
     CDS             complement(<1..447)
                     /locus_tag="PLG78_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNE23180.1"
                     /translation="MTDGAFLGLTQEEAEVLLAQNGPNEIATEKRESSIRRFLTSLRN
                     PLVLLLLFLATVSYATGDLRATIVILVMVLLGVVLRFFQEERADRAAEKLKKLVGSTA
                     TVIRDGVPHEIPFAKLVVGDLVHLAAGDMVPADVRVVQAKDLFLNQA"
     gene            502..1023
                     /locus_tag="PLG78_08435"
     CDS             502..1023
                     /locus_tag="PLG78_08435"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE23181.1"
                     /translation="MLKKILKWVGIVVGVFVLVGLLLPRAAKLERSIVIQAPADKVFV
                     YVNSLEKFQEYSPWRDMEPEAAVTMGEKKEGVGASFSWKGKKTGEGSMTITESVKNER
                     VVTALDFKDQGKAGATFLLKPQGAETQVTWTFEGDAGYNLISRYFYLFIDKLLGSDYE
                     KGLAKLKKVVEKK"
     gene            complement(1102..2406)
                     /locus_tag="PLG78_08440"
     CDS             complement(1102..2406)
                     /locus_tag="PLG78_08440"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNE23182.1"
                     /translation="MKLILAALIAFSACVPSRAEPISSGPMAGNQSPRTVTLWLQTNG
                     VESGEIKYSNVKLKGVPRSFKFTTSSEDDYALHAELSDLEPASTYTYEIIFKSKTYGP
                     FEFRTLRNGKSEEEFRVLTGSCAYINDSRFESGKPYGAHYEIFETMAKANPDVMLWLG
                     DNSYLRDVDFLNPWSMNQRFRKDRSLPQMQALLHKGHHYAIWDDHDYGPNDSTSSFIF
                     KDHSLKFFKRYWANPTYGLNETPGIFTSFRKGDAEFFLLDDRFNRDADRSPNRPEKQM
                     FGPAQMRWLKNALVNSNASFKIIAGGSQMFNDANKFEGWQNFPQERKDFLDFVAEARI
                     TGLFFLTGDRHHTELAKIERPGTYPIYELTCSPLTSGIHPVSEEEKKNGKHLPGTLIE
                     ERNFCQLDFGGAGSDRRITVSSFDPTGKMLWEKKIQLSELGK"
     gene            complement(2403..2771)
                     /locus_tag="PLG78_08445"
     CDS             complement(2403..2771)
                     /locus_tag="PLG78_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HNE23183.1"
                     /translation="MKRSPALVFLLGLCTLGLYLLFWYNRIYNELKELGGKTPTGNSY
                     GFDLLLTIVTCGVWGIYVDYRISLTLDEILVARSMPAQNSAPLVLVLDIFFYVTGGLT
                     YLATSAIHQDIINRVYEQKA"
     gene            complement(2771..3190)
                     /locus_tag="PLG78_08450"
     CDS             complement(2771..3190)
                     /locus_tag="PLG78_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNE23184.1"
                     /translation="MKRIQFNEFEVGAKLPELKVGPVQHMDLVRYSGASGDFNPIHTD
                     PTFAKSVGLDGTIAHGMYVMAQVGRMCTNWVHPAQIQFYGIKFKGMTKPGEMIVCTGE
                     VKKKKEEDGKKLMTVSVSAANEAGEVKAAGDLVVVCD"
     gene            complement(3201..3647)
                     /locus_tag="PLG78_08455"
     CDS             complement(3201..3647)
                     /locus_tag="PLG78_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNE23185.1"
                     /translation="MALSKDIVGKKLDSYQFTVERGKVKEFCRAIGETNPIYTDPAEA
                     KKAGFQDTPIPPTMQTTFQFWGYPKIWDDMKGMGIDISRLLHMKEEYTYLKPVYPGTT
                     IHAAGEVIDVKTGKMDMVTFKTTYANEKGEPCILAEMAIVIRPEGT"
     gene            3745..4392
                     /locus_tag="PLG78_08460"
     CDS             3745..4392
                     /locus_tag="PLG78_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNE23186.1"
                     /translation="MMVQRTTKRKILGMLAVLGFFSLVRDHWAEPEERLLQRSFLTGY
                     IQRTHPQIHSVDRDRLVLAILDQAGGLALSDQKIDGAPVDRVLFLTAVIETESTFKSD
                     AISSMDARGYMQLLPSTAKWMGDKFGLGVPATQDLFRPEVNLPRGVIFLNHLNEQLRD
                     IRLVCLAYNAGLGNVQRGYWDERYWQKIYINYRRLSSEAEDNRWMSREVTGVRDI"
     gene            complement(4403..5284)
                     /locus_tag="PLG78_08465"
     CDS             complement(4403..5284)
                     /locus_tag="PLG78_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23187.1"
                     /translation="MRTRLFLLFFSSVCAVTAVYANMRQRWQVDRYPSYFPQSVPGTT
                     VDSEDLNFICDLAYEGDADWSEMQKRGCNVRALYAITTKIERGVALEFILPSEDPVTV
                     FINGEKVKVDSREIVMTDTTRRAYRLSEVCHFCGSKFSRLYAASFDGKLRAGKNEIEV
                     QYRQPYSFYETAYGYFKTSEWLNSFEYELWPLRSWEVSPGFKMNIRVQLQRPGWLKRI
                     TTDSKLECSGENLQIPTHFKLPPQSTSENNFESWIKQNTRTDVKLNRRNEADQAIFTT
                     TMTENFPDRLACIVQSD"
     gene            5356..5832
                     /gene="nudB"
                     /locus_tag="PLG78_08470"
     CDS             5356..5832
                     /gene="nudB"
                     /locus_tag="PLG78_08470"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF006961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNE23188.1"
                     /translation="MGWVIPLAQPTPLTSPMHYKVPESALVVVYSKDQFLLLERSDRP
                     GYWQSVTGSRDVHETIEQTAARELKEETGLTMADGVLISTGRTHTYPIMDEWRHRFAP
                     GVSENRETVFYFSMPQVCNVTLSEHIRYEWVSGEEAVGRVFSRTNREEIQRILMTV"
     gene            5883..6809
                     /locus_tag="PLG78_08475"
     CDS             5883..6809
                     /locus_tag="PLG78_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE23189.1"
                     /translation="MAKKKQAIPKKAARKTYELQRVNFPSGSGGCEADLYIPKGRGNH
                     PVVVMAHGFGAERSFRLPAFADRFAEAGIAVLLFDYRSFGDSPGEPRNLISPRMHNED
                     WKAALQFVRRMPGLDSQRIALWGTSFSGGHIVNIAPEEKPAALSLLVPFSDGFRTLFN
                     YSAAFVLQAVFHGLWDLLLSLFGRSHNVPIIGKPEEFALLNTAECWEGYGGIVDKESA
                     WQNSAPARICLTLPMYRPVAKASRIKCPVIIVAGEQDSLCPLPLVQTLARKIRGSMMM
                     LLSTGHFEPYSGRAFEETVRAQLQFFRQCFVR"
     gene            6855..>7063
                     /locus_tag="PLG78_08480"
     CDS             6855..>7063
                     /locus_tag="PLG78_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23190.1"
                     /translation="MKNRLSLSFVLGLPFAALLTSASILIAQSQPSVQVFSPQGIVKK
                     PRQVRVQFSEAMVPFGFLKQPVKPF"
BASE COUNT         1659 a         1694 c         1807 g         1903 t
ORIGIN      
        1 ggcttgattt agaaagagat cctttgcctg caccacgcgc acgtctgccg gaaccatatc
       61 acccgcggcc agatgcacta aatcgcctac caccagttta gcgaatggga tttcatgagg
      121 aacaccgtcg cgaatcacgg ttgcggtcga gcccacaagt ttcttgagtt tttccgcagc
      181 ccggtctgct ctctcttcct gaaaaaaacg aagtactacg cccaggagca ccatcaccag
      241 gataacaatg gttgcgcgaa gatcgccggt tgcgtaggaa acagtcgcta aaaacagaag
      301 gagcaggacg agagggtttc gcaggctcgt caggaatcgt ctgatgctgg actcgcgctt
      361 ttcagtggca atttcatttg gcccattttg cgccaggagc acttctgctt cttcctgggt
      421 caggccgagg aatgcaccgt ctgtcatggg aacatctcac tttcgcttgc agaaagttca
      481 agcgcattgc aaacgtggtt catgcttaag aagattttga aatgggtcgg gattgtagtc
      541 ggtgtgtttg ttcttgttgg attgctcttg cctagagctg cgaaactgga acgttccatt
      601 gtcattcagg ctccggcgga caaagtgttc gtctatgtga atagtctgga aaaatttcag
      661 gaatactctc cgtggaggga tatggagcca gaggctgccg tcacgatggg agagaagaaa
      721 gaaggcgtgg gcgcttcctt tagctggaaa ggaaagaaga caggcgaagg aagcatgacc
      781 atcactgaaa gcgtgaagaa tgagcgagtg gtaactgcgc tcgatttcaa ggatcagggg
      841 aaggctggcg cgacgttcct gctaaaaccg cagggcgctg aaactcaggt cacctggact
      901 tttgaagggg acgctggcta caatttgata tccagatatt tctatttgtt catagacaag
      961 ctacttggat cagactatga gaaggggctt gcaaaactca agaaagttgt agagaagaag
     1021 taagttgcac caggcatcac gatagttcag gaactacgat tttttcgcat gaatcgcctt
     1081 cgggcgattc tacgcgaaga gttatttgcc cagttctgaa agctggatct ttttttccca
     1141 cagcatcttg cccgttggat caaacgatga taccgttatg cggcgatcag atcctgctcc
     1201 cccgaaatcc aactggcaga aatttctctc ttctatgaga gtgccgggta aatgctttcc
     1261 atttttcttt tcttcttcac tgactgggtg aatcccgctg gtcagagggg aacacgttag
     1321 ctcatagatc ggataggtgc caggacgttc aatcttcgcc agttcagtgt gatgtctgtc
     1381 tcccgtcagg aaaaagagac ccgttatgcg cgcttccgcg acgaaatcca ggaagtcctt
     1441 tctttcttgc gggaaatttt gccagccttc gaatttgttt gcgtcgttga acatctggct
     1501 tccgccagcg atgatcttga aagatgcatt tgagttaacc agtgcatttt tcagccatcg
     1561 catttgagca ggaccaaaca tttgcttttc cggtcgattc ggagatcgat ccgcatcgcg
     1621 gttgaatcgg tcatcgagca aaaagaattc tgcgtcgcct ttgcgaaaag aggtgaatat
     1681 gcctggggtt tcgttcaatc cataggttgg gttggcccag taacgcttga aaaacttaag
     1741 gctgtggtct ttgaaaatga aactcgatgt ggagtcattg ggaccatagt catgatcgtc
     1801 ccagatggcg taatgatgcc ccttgtgcag cagtgcttgc atttgaggga gagatctatc
     1861 tttgcgaaag cgctgattca tactccaggg attcaagaaa tccacatcgc gaaggtaaga
     1921 gttgtctccg agccagagca tgacgtcagg atttgctttt gccatggttt cgaatatttc
     1981 gtagtgggcc ccgtagggtt ttcctgattc aaagcgagaa tcgtttatgt aggcgcagga
     2041 gccagtcagg actctgaact cctcttcgct tttgccattg cgtagagtgc gaaattcgaa
     2101 tgggccgtat gtttttgatt tgaaaatgat ttcgtaagtg taagtggatg ctggctctag
     2161 atcagaaagc tctgcgtgca acgcatagtc gtcctcagaa gaggttgtga atttaaaaga
     2221 ccgcgggacc cctttgagtt ttacattgga gtatttgatt tcccctgatt ctactccatt
     2281 cgtctgcagc cagagtgtga ctgtgcgagg agactgattg cccgccatgg gtccagagga
     2341 gatcggctct gcccgcgaag gaacgcaggc agagaacgcg atgagggcag caagaattag
     2401 tttcaagctt tctgttcgta aacccggttg atgatgtctt gatgaatggc agaggttgcc
     2461 agatacgtca aaccacccgt tacatagaaa aagatgtcca ggaccagaac cagaggagct
     2521 gaattctgtg caggcatgct gcgcgccacg aggatttcat cgagagttaa agatattcta
     2581 tagtctacat agattcccca gacaccgcat gtaacaatgg tcaggagaag atcaaagccg
     2641 tacgaatttc cagtgggtgt ctttccgccc agttctttga gttcgttgta gatgcgattg
     2701 taccagaata gaagatacaa tccaagagta caaagaccaa gtaagaatac aagtgcagga
     2761 gagcgtttca tcagtcgcac accactacaa gatcgcccgc tgctttcact tcgccggctt
     2821 cattggccgc ggaaacagaa acagtcatca gtttcttgcc atcttcttct ttcttctttt
     2881 tgacttcgcc tgtgcagacg atcatttcgc ctggctttgt cattcctttg aacttgattc
     2941 catagaattg aatttgagcc gggtgcaccc agtttgtgca catacgcccc acttgcgcca
     3001 tcacatacat accgtgcgcg atggttccat ccagtcctac gcttttagcg aaagtaggat
     3061 ctgtatgaat cggattgaag tcaccggatg cgccggaata gcgcaccaga tccatgtgct
     3121 gcaccggccc gactttcagc tccggcagtt ttgcgccgac ttcgaattcg ttgaattgaa
     3181 tgcgtttcat gttttgctcc tcaagtccct tccggacgaa tcacgattgc catttctgcc
     3241 agaatgcagg gttcgccttt ttcattcgca tacgttgttt tgaatgtaac catatccatc
     3301 tttccagttt tcacatcgat gacttccccc gctgcgtgga tggtcgttcc ggggtaaact
     3361 ggtttgaggt aagtatactc ttctttcata tgtagaagac ggctgatgtc gattcccatg
     3421 cctttcatgt cgtcccagat cttggggtat ccccagaact gaaatgtagt ctgcatggtg
     3481 ggaggaatag gagtgtcctg aaagcccgct tttttggcct ccgccgggtc agtgtagatc
     3541 gggttggttt ctccgattgc gcggcagaat tcttttacct ttccgcgttc caccgtaaac
     3601 tgatacgaat cgagcttttt gcccacaatg tctttgctga gtgccatatc gctttgtggg
     3661 atcgcacctt ttcgggtcaa gctacatgca ttctggaccc caaaaaggac tttacctggt
     3721 gcaacgcaaa aaaatcatgg cttcatgatg gtgcaacgca caacaaaacg caagatcctg
     3781 gggatgctgg cagtgctcgg cttcttttcc ctggtccgcg accactgggc ggaaccggaa
     3841 gagaggctgc tgcagcgatc ttttctgact gggtacatcc agagaaccca tcctcaaatt
     3901 cattctgtgg atcgtgatcg gttggtcctg gccattctgg atcaggccgg cggtctggct
     3961 ctttcggatc aaaagatcga tggggcccct gtggatcggg ttctgttttt gactgcggtc
     4021 atcgaaacag aatccacttt caaatccgac gcaatctcct ctatggacgc gcgcggatac
     4081 atgcaactgc tcccgtctac tgccaagtgg atgggcgata agttcgggct gggagtacct
     4141 gctacgcagg atttgtttcg tccagaagtg aaccttcctc gtggcgtgat cttcctcaat
     4201 catctcaatg agcaattgcg cgacattcgc ctggtctgtc tcgcatacaa tgccggtctg
     4261 ggcaatgtgc aacgcggata ctgggacgaa agatactggc aaaagatata catcaactat
     4321 cgcaggcttt cttcggaagc agaagacaat cgttggatga gccgggaagt tacgggagtg
     4381 cgcgatatct agtcgcgcat tctcagtccg attgcacaat gcacgcgagg cgatcaggaa
     4441 aattttcagt catcgtagtc gtgaaaatgg cctgatctgc ttcatttctt cgattcaatt
     4501 taacatcggt tcgcgtgttt tgtttgatcc atgattcgaa gttattttcg gatgtgcttt
     4561 gaggcggaag cttgaagtga gtcgggattt gcaggttctc accgctgcat tctagtttgc
     4621 tgtctgttgt gattcttttc aaccatccgg gtctctgcaa ttgcactcgg atgttcattt
     4681 tgaacccggg ggacacttcc cagctgcgca gaggccatag ttcgtattca aatgaattga
     4741 gccattcact tgtcttgaag taaccgtatg ccgtttcgta gaaactgtag ggttgtctgt
     4801 attgtacttc aatttcattt tttccagctc gcaatttccc gtcaaaggac gcagcgtata
     4861 gacgcgagaa ttttgatccg cagaaatggc agacttcact caggcgatac gcgcggcgtg
     4921 tggtgtctgt catgacaatc tctcgtgaat ccactttcac tttttctccg ttgatgaaca
     4981 cggtcacagg gtcttccgac ggtagaatga attccagcgc cacacctcgc tctatctttg
     5041 ttgtgattgc atacaaagcg cgcacgttgc atcctctctt ttgcatttcg ctccaatctg
     5101 catcaccttc gtaagcaagg tcacagatga aattgagatc ttctgaatct acagtggttc
     5161 ccggcacact ctgaggaaaa tacgaggggt aacgatccac ctgccagcgt tggcgcatgt
     5221 tggcatacac tgcagtgaca gcgcaaacgg aggagaaaaa gagaagaaag agccttgtgc
     5281 gcatgcctga aaataattga agtctttagc ccggaaagtg cttttcagcc tgctcgggtc
     5341 cgatatattg acagtgtggg ctgggtgatc cccctcgctc agcccacacc tcttacatct
     5401 cccatgcact acaaagtccc agaatccgct ctggtcgtgg tgtattcaaa agatcagttt
     5461 cttctattgg aaagatcgga caggcctggc tactggcaat cggttacggg gtctcgcgat
     5521 gtgcatgaaa cgatcgaaca gactgctgct cgtgaattga aagaagaaac gggcctcaca
     5581 atggcagatg gtgttctaat ttctacggga cgcactcata catatccaat catggatgaa
     5641 tggcgccatc ggtttgcgcc cggtgtatct gaaaacaggg agacagtgtt ttatttttcc
     5701 atgcctcagg tttgcaacgt cactctgtcg gaacacattc gatacgaatg ggtttcaggg
     5761 gaagaggccg tcggtagagt cttctcaaga acaaatcggg aagagatcca gcggattctc
     5821 atgactgttt gattggtcta gaaatgaagt gcgctccaaa acgactgcgg tgcgattgac
     5881 acgtggccaa aaagaaacag gcgattccta aaaaagccgc gcgaaaaacg tacgagttgc
     5941 agcgtgtaaa ttttccgtct ggttccggtg gttgcgaagc ggatctttat atcccgaagg
     6001 gtcgtgggaa tcatccggtg gttgtgatgg ctcatggttt tggagctgaa agaagtttca
     6061 gacttcctgc gttcgcggat cgcttcgcgg aagctggaat cgcagtgctc ctgtttgact
     6121 atcgaagctt cggagatagt cccggtgagc cgcgaaatct gatcagtccg cgcatgcaca
     6181 atgaagactg gaaagcggca cttcaatttg tccgcagaat gcctggcctc gattcgcagc
     6241 gcatcgctct ctggggaact tcttttagcg gtggccatat tgtgaacatt gctcctgagg
     6301 aaaaaccagc cgcactttca ctgctggttc cattctctga tggctttcgc actcttttta
     6361 attacagcgc agcttttgta cttcaggccg tgtttcatgg cctgtgggat cttttgcttt
     6421 cactctttgg gagatcgcat aacgttccga tcataggcaa accagaggag ttcgcacttc
     6481 tcaacaccgc ggaatgctgg gaaggatacg gtggaatcgt agacaaagaa agcgcctggc
     6541 aaaactcagc tcccgcgaga atctgtttga cccttccgat gtatcgccct gttgcgaaag
     6601 cgtccagaat caagtgcccc gtcatcattg tagccgggga gcaggattcg ctctgtcctc
     6661 tgcctctcgt tcaaactctg gcccgcaaga ttcgcggatc catgatgatg cttttgtcca
     6721 ccgggcattt tgaaccatac agcggccggg catttgaaga aacagtaaga gctcaattgc
     6781 agtttttccg gcagtgcttc gttcgctgat tttgttgaca ctggttttcg cagcttgctt
     6841 ctaaagcaga agctatgaaa aatcgccttt cgctctcctt tgttctgggt cttccgtttg
     6901 cggcccttct caccagcgcc tccattctca tcgcgcagtc gcaaccgagc gttcaggttt
     6961 tttctcctca aggaatcgta aaaaagccgc gccaggtgcg cgtgcaattt tcggaagcca
     7021 tggtgccatt cggttttctc aaacaacctg tgaaaccgtt tga
//
LOCUS       DAOQJL010000154         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_5978_length_7005_cov_3.877122, whole genome shotgun sequence.
ACCESSION   DAOQJL010000154 DAOQJL010000000
VERSION     DAOQJL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_7005_cov_3.877122"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLG78_08485"
     CDS             <1..190
                     /locus_tag="PLG78_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE23191.1"
                     /translation="MQNVRMQGILVGSRDSLASVARAYEIHKVRPVIDRTFSFDQTPA
                     AIQYLADGKHFGKVCITI"
     gene            263..1519
                     /locus_tag="PLG78_08490"
     CDS             263..1519
                     /locus_tag="PLG78_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23192.1"
                     /translation="MRILIFLLLMPLSLVADTVRLRDGSVLTGKVTCQDPSSITLESK
                     DGVQSISKSEIAAFREDDSGTESYKRRLADARAASETRQKQIDQAIADRRQAVPTGFW
                     IGMGAGSGRIYFPQESILIHDRIEASLIPNGAQANAFPGFARRPANGEDANAGYQGDR
                     FYVEADGLSYRNHLETAALGLPSLSSGGLNSSSSTQFWSLKGFGQLQQASLVAGVEPF
                     RFSERFRIFLFAGKTGFNSTTQFSGLLFSPLVFTPSFYLTGFIQNFRLRLYGEGPRGG
                     VQLRFEPAVRWEIRGQAAVASFAGNSSEHVTVMFGPFNIPGVTYQLPTKISTRGSLWV
                     RQVEYTLTGFFALPSRVRLFLSYMAQDVHIQYGSRTNQNFPADLFLSAYFSQARFSEV
                     ILRQYDHPRGNGRFRRVSAGAEFRIY"
     gene            1497..2612
                     /locus_tag="PLG78_08495"
     CDS             1497..2612
                     /locus_tag="PLG78_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE23193.1"
                     /translation="MLNSGSTEKGKARFRLVGEVKNTLIFPAHELHSSMADSMNPSIA
                     ILGLGAIGASIARALYLNRIPFTLLARDQGRLAELNRGFLFTTGRKRVEINSSSGVMK
                     ASLISSGETYDRIFLGMKSVHLPESVVAARKLLTPSGKLILLQNGIPLDLLPSASAAF
                     CMDGIVGYNVQIKDGSYYQSNAGHLILGEDGRMPDKDLKLALEPHLPVILTDNAKGFR
                     WNKLAINCVINGLGAISGEPLGPIFGNKHGRELAIQLLEETGSVIKAMGVHEEIVPGG
                     ISVFKFGRGGWPRIVQHSVLRALGLKYRQIKTSMLQDIENHRPTEVRSIHGAVLDAAR
                     KVNVAVPRMKAVVETIGYIEKGEMSPYPGLLSELAKA"
     gene            2734..3861
                     /locus_tag="PLG78_08500"
     CDS             2734..3861
                     /locus_tag="PLG78_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23194.1"
                     /translation="MKQYDALTALVVYYNVTRACSFSFQSVTQPAVWQFVKSELESQS
                     VKGHLQGESVTTFGPAAPGTGAYSGAVLAPNGKIYLVPGSATSYRVIDPVAGTVSTFG
                     PAAPGGSAFRGGVLAPNGKIYLIPFNATSYRTIDTATDTVGSFGTAPGGAAFQGGVLA
                     PNGRIYMAPFNSTTYSYIDPTNDTVSSFGPAAPGSSAYHGAVLGPNGKAYLIPAANTT
                     FRYIDTFTDTVFALGTAAPGGDAFHGGVLASTGRIYLVPYLSTTYRYIDTSNDSVVTF
                     GPAAPGSNAFDGGVIGPGGRIYLVPHDAGNFYYIDPGTGTVATYGPGGLPPGAFMGGI
                     VTSTGRVYFAPYNEATYRYTDTGAAGSLCTSILLSGYLNKY"
     gene            3973..4734
                     /gene="fabG"
                     /locus_tag="PLG78_08505"
     CDS             3973..4734
                     /gene="fabG"
                     /locus_tag="PLG78_08505"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNE23195.1"
                     /translation="MSINLTGKAAVITGSARGIGKAIALKLASLGADVVISDINAEQA
                     AATAKEIASATGKRAISHAGDISKLDQAEALIDACVKEFGHIDILVNNAGITKDTLLM
                     RMKPEQWDAVIAVNLTGTFNCTQPAFKQMMKQRSGAIVNISSIAKDGNMGQTNYSASK
                     AGVVGFTKAVALEGASRGIRSNAIAPGFIKTDMTDAIPEAIRSRMVKMIPLQRAGLPE
                     DIANGVAFLSSDMASFITGHVLEINGGGFQPASAD"
     gene            4884..5120
                     /gene="acpP"
                     /locus_tag="PLG78_08510"
     CDS             4884..5120
                     /gene="acpP"
                     /locus_tag="PLG78_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE23196.1"
                     /translation="MADMEKIKAIIVEQLGVDESEVTPEAHFIDDLGADSLDTVELVM
                     ALEEEFGIEISDEDAEKIQTVGDVSRYIDEHASK"
     gene            5165..5914
                     /gene="rnc"
                     /locus_tag="PLG78_08515"
     CDS             5165..5914
                     /gene="rnc"
                     /locus_tag="PLG78_08515"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNE23197.1"
                     /translation="MGIRLFRKTQDLPPDRKARLAVFAKKTHLPFKDLTLLHGALVHS
                     SFAHEQKPPIPDNQRLEYLGDSVLGLIINDSLFRSYQNMDEGRMARIKASLASEQTLA
                     RAASRISLGEVLLLGRGEKQTGGKTRSSILADALEAVIGALYVDRGWQAVYTFVTELL
                     ADEIEKLSGREDVRDPKTKLQEYLQKQGKALPEYKLLGKIGPDHKSTFQISVSLSGKE
                     IAQGTGESRKKAEHQAASNALKKFHRMRIGN"
     gene            5928..>7005
                     /gene="aroB"
                     /locus_tag="PLG78_08520"
     CDS             5928..>7005
                     /gene="aroB"
                     /locus_tag="PLG78_08520"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNE23198.1"
                     /translation="MEAAANIRLPGSAPYEYPIFIQESYAGLAQILRERPGKLFVISQ
                     KGLESVLDLFFRESGVPRDRLYLIEQGEENKHLNQLGEVYNWLIQSGIDRSSSIIAPG
                     GGVVGDFAGFVASTILRGVPFIQIPTTLLASVDSSVGGKVAVNADFGKNMVGSFYHPA
                     FVYCNVSVFSTLPEDEWSCGFAEIVKHSFLEETSRDLNYLEQNVQNIRKPEILSLVIR
                     DNVRFKASIVSQDEKEAGLRSILNLGHTTAHALESITHYKKFSHGQAVSRGLVTALLL
                     SEKTGLSATDRERMLSLMQKLALPLDTAGFGAAELLEHMKFDKKNTGGQIRFVLLEKP
                     GKPVFGIPITAEEFENAWSAQKRLF"
BASE COUNT         1724 a         1779 c         1795 g         1707 t
ORIGIN      
        1 gatgcagaat gtgcgcatgc agggaatact cgtgggaagc cgcgacagtc tggcttctgt
       61 ggcccgcgca tacgaaattc acaaagtcag gccagtcata gatcggactt tttcgtttga
      121 ccagactccg gcggccatcc agtatctggc agatggcaaa cactttggca aagtctgcat
      181 cacaatctga agctcaaagc gcgccacggt tcgctgagaa acgcggacac atcatcgaaa
      241 aagtcgtctg atcggggttc atttgcgcat ccttatcttt ctattgctga tgcctctttc
      301 gctcgtggcg gacaccgtgc gcttgcgcga cggttctgta ctcaccggta aagtcacgtg
      361 ccaggatcca tcgtcgatca ctcttgagtc aaaagacggc gttcaatcaa tctccaaatc
      421 agaaattgcg gcattcagag aggatgactc cgggaccgaa tcatacaagc gcaggcttgc
      481 ggatgcgcgc gctgcatcgg aaaccagaca aaagcaaatc gatcaggcga tcgcagatcg
      541 caggcaagct gttcccactg gattctggat cggaatgggc gcgggttcgg ggcgaattta
      601 ttttccgcag gaatccattc tcatccatga tcgcatcgaa gccagtttga tcccaaatgg
      661 agcgcaggcc aatgcgtttc caggttttgc gcgacgtcct gcaaatggtg aggatgcaaa
      721 tgcaggctat cagggagatc gcttctacgt ggaagcggat ggtttatctt atcgcaatca
      781 tctcgagacg gcggcactgg gcttgccttc tttgtcctct ggcggactca attcaagttc
      841 aagcactcag ttctggtcac tgaaagggtt tggacaattg cagcaggctt ccctggtggc
      901 cggcgtggaa cctttccggt tttctgaacg attccgaatt ttcttgttcg caggtaagac
      961 tggatttaac tccaccactc aattcagcgg cttgttgttt tcgcctctcg tctttacgcc
     1021 ttctttctat cttacgggtt tcattcaaaa ttttagattg cggctctatg gagaaggtcc
     1081 acgcggaggc gttcaacttc gttttgagcc cgcagttcgc tgggaaatcc gaggtcaggc
     1141 agcagtcgcc tccttcgcag gtaattcatc cgaacatgtt acagtaatgt tcgggccctt
     1201 caacattccg ggcgtcacct accagctgcc caccaaaatc agtacccggg gctccctgtg
     1261 ggtccggcag gttgaataca ctttaacggg ttttttcgcg cttccatccc gcgttcgcct
     1321 ttttctcagt tacatggccc aggacgttca cattcagtac ggcagtcgga ccaatcagaa
     1381 tttccctgcc gatttgttcc tgagcgcgta cttttcgcag gctcggttca gcgaagtcat
     1441 cttaaggcag tacgatcacc cgcgcggcaa tggacgcttt cgccgcgtga gcgcgggtgc
     1501 tgaattcagg atctactgag aaggggaagg cacgctttcg cctcgtggga gaagtgaaaa
     1561 atactttgat ttttccagca catgagctac acagttctat ggccgattcc atgaatcctt
     1621 ccattgcaat tcttggtctc ggagccatcg gtgcaagcat cgcacgcgcc ctctacctaa
     1681 accggattcc gtttacattg ctggcgcgtg atcaaggcag gcttgcggag ttgaatcgcg
     1741 gctttttatt cacaacaggt cgaaaacgtg tggaaataaa ttcgtcttcc ggtgtgatga
     1801 aagcgtcact gatttcatcc ggagaaactt atgatcgcat cttccttgga atgaagagtg
     1861 ttcatctgcc ggagtcagtg gtcgcagcgc gaaagctcct gaccccatcc ggaaaactga
     1921 tcctgctgca aaatgggatt ccactggatc ttcttccttc tgcttctgct gccttctgca
     1981 tggatggaat tgtgggatac aatgtgcaaa tcaaagatgg ttcctattac cagtcgaatg
     2041 cggggcattt gattctgggc gaagacggaa ggatgcctga caaagatttg aaattggctc
     2101 ttgagcccca tttgccggtg attttgacag acaatgcgaa aggctttcgc tggaacaaac
     2161 tcgccatcaa ctgcgtgatc aatggtctgg gagccatcag cggggaacca ctcggcccta
     2221 tttttggaaa caaacacgga agagagctgg ccattcaact tctagaagaa acgggcagcg
     2281 tgatcaaagc gatgggagtt catgaagaga ttgtgcccgg tgggatttct gttttcaaat
     2341 ttggcagagg tggttggccg cgcatcgttc agcatagcgt gcttcgagct ctcggactca
     2401 agtacagaca gatcaagaca tccatgctgc aggacataga aaaccacaga ccaactgaag
     2461 tgcgaagcat tcacggtgct gttctggatg cagccagaaa agtgaatgta gcagttccgc
     2521 gcatgaaggc agttgtagaa acaatcggtt acattgaaaa gggcgagatg tctccttatc
     2581 ctggtttgct ttctgaactg gcaaaggcct gaaagtttcg catgaaagaa aggattaccg
     2641 ttgcacagca tcttacgtaa aactagactt cgctcgtttt catttttgat cgggttcttt
     2701 ttcctttcgg cctgcgctcg atttcctgta gatttgaagc agtacgatgc tctaactgca
     2761 ttggtagtct attataatgt cacacgcgcc tgttcgtttt cgtttcaatc cgtaacgcag
     2821 cctgcagtct ggcagtttgt gaaaagcgaa ttggaatcgc agtctgtgaa aggtcatttg
     2881 cagggggagt ccgtgactac atttggtccg gctgctcccg gaaccggtgc atacagcgga
     2941 gcggtccttg cgcccaacgg gaagatttac ctggttccag gttcagccac cagctacaga
     3001 gtgattgatc ctgtggccgg aacagtgagc acatttgggc ctgcagcacc tggaggctct
     3061 gcgtttcgcg gcggagtgct cgcgcccaac ggaaagatct atttgattcc tttcaatgct
     3121 acgtcgtatc gcaccatcga tactgccacg gatactgtag ggagttttgg aacagcgcca
     3181 ggaggagctg ccttccaggg cggagttctt gcgccgaacg gtcgcatcta catggctcca
     3241 ttcaacagca ctacgtattc ctacatagac ccgaccaatg acacagtatc ctcgtttggc
     3301 cccgcagccc ctggttcgag cgcttaccac ggcgcagttc tgggaccaaa cggaaaggcg
     3361 tatctgattc ccgcggccaa cacaacgttt cgctacatag atacattcac tgataccgta
     3421 tttgcgttgg gtacggcggc acctggaggc gacgcatttc acggaggagt tcttgcatct
     3481 accggtcgca tttatcttgt tccgtaccta agtactacgt atcgatacat tgacacctca
     3541 aacgattcgg ttgtgacgtt tggtccggca gcacccggaa gcaatgcttt tgatggagga
     3601 gtcattggcc cgggcggacg aatctacctc gtacctcatg atgccggtaa tttttactat
     3661 atagatcctg gaacaggaac cgtagcaacg tacggcccgg gaggtcttcc tccaggtgcc
     3721 tttatgggcg ggatcgttac ttccacagga agagtctact tcgcgcctta caacgaggct
     3781 acttatagat acacggacac cggcgccgcc ggatccctct gtacgtcgat tctactgagc
     3841 ggttatctga acaaatactg atcgcctgtt tgcctggcgt tcctttcgcc caaaaacgct
     3901 ggtcatgagc aagttcttca gagaattgga ccaagacggc acaatgccgt cgcaacaatc
     3961 ggggagtctt ccatgagcat caatcttacc ggcaaagcag ccgtcattac cggttctgct
     4021 agaggaatcg gcaaagcaat cgcactgaaa ctggccagcc tgggcgcaga cgtagtcatc
     4081 agcgatatca atgcagagca ggcagcagcc acagcaaaag aaatcgcatc ggccactgga
     4141 aaaagagcca tctcacacgc aggagatatt tcaaaactcg atcaggcaga agcactcatt
     4201 gacgcatgcg tcaaggaatt cggccatatt gatattctgg tgaacaatgc agggatcaca
     4261 aaagatacac tgctcatgcg catgaagcca gagcaatggg atgctgtgat tgcagtgaac
     4321 ctgactggaa ctttcaactg cacccagcct gctttcaagc agatgatgaa gcagcgttcc
     4381 ggtgcaatcg tgaacatttc ttctattgca aaagacggca acatgggcca gaccaactac
     4441 tccgcctcca aggcaggtgt ggtaggattt acaaaggcag tggcactcga aggcgcaagc
     4501 cgcggcatca gaagcaacgc aattgcaccc ggtttcatca aaacggacat gacagacgca
     4561 attccggaag cgattcgcag ccgtatggta aagatgattc cacttcagag agctggactc
     4621 ccagaagaca tcgcaaacgg agtcgcattt ctatcctctg atatggcgtc tttcatcaca
     4681 ggccacgtac tggagatcaa cgggggcggt tttcagccag cttctgcaga ctaatcacag
     4741 tttttgcacg tgcgaagaat ccgtggtttt ttcaagacca cggatttttt tttggttttg
     4801 cacttgcccg cgcaaagggc atggtcattt tgagcacctc gtacctacta caggggtacc
     4861 aatcaaaaac gtggaggcct ataatggctg atatggaaaa aatcaaagct atcatcgtcg
     4921 agcagcttgg agtagacgaa tcagaagtca ctccagaagc tcacttcatt gacgatctcg
     4981 gcgctgactc gctcgacact gttgagcttg ttatggctct tgaagaagag ttcggcatcg
     5041 aaatctctga cgaagatgct gaaaaaattc agacagttgg cgacgtatct cgttacatcg
     5101 acgagcacgc ttccaagtaa tctgctgcgc cggcaatcgc aagattgccg gcacttttct
     5161 ctgaatgggc attcggctgt ttcgtaagac gcaagatctg ccccctgacc gcaaagccag
     5221 actggctgta ttcgcgaaaa agactcacct acctttcaaa gacctgaccc tccttcacgg
     5281 cgcgcttgtg cactccagct tcgcgcatga acaaaaacct cccataccgg ataaccagcg
     5341 tctggaatat ctgggagact ccgtgcttgg cctcatcata aacgattcac tatttcgcag
     5401 ttatcagaac atggacgaag gaagaatggc acgcatcaag gcaagcctcg cgtccgaaca
     5461 aactctcgcg cgtgctgctt cgcgcatttc tctcggagaa gtattgctgc ttggccgtgg
     5521 agaaaagcag acaggcggaa agacccgctc atcgattctg gcagatgctc tcgaagctgt
     5581 gatcggtgca ttgtatgtgg atcgcggctg gcaagctgtc tatacttttg tgacagaact
     5641 gcttgcagac gaaatagaga aactctccgg acgcgaggat gtccgggatc ccaaaaccaa
     5701 gcttcaggaa tacctgcaga aacagggcaa agcactgccc gaatacaaac tgcttggaaa
     5761 aataggacca gatcacaaga gcacttttca aatttcagtt tctctttctg ggaaagaaat
     5821 tgcccagggc acgggtgaat cgcgcaaaaa ggcagaacac caggcagcca gcaatgccct
     5881 aaagaagttt caccgcatgc ggattggaaa ttgatggtaa acagatgatg gaagcggcgg
     5941 caaacatcag acttccggga agtgctccct acgagtatcc cattttcatt caggaatcgt
     6001 acgccggtct cgcccagatc ttgcgcgaaa gaccaggcaa actttttgtc atcagccaga
     6061 aaggtcttga atcggtcctg gatttgtttt tccgcgaatc gggtgtgcct cgcgatcgcc
     6121 tctatttgat agagcaggga gaagagaaca aacacttgaa ccagctcggt gaagtctaca
     6181 actggttgat ccagtcagga atcgatcgct ccagttctat catcgcccct ggcggcggcg
     6241 tcgttggcga tttcgcagga tttgtcgcat ccacaattct tcgcggagtt cctttcattc
     6301 agattccaac cactctgctt gcttctgtgg attcgagcgt gggcgggaaa gtggcagtga
     6361 atgccgattt cggaaagaat atggttggtt ctttttatca tccggccttt gtatactgca
     6421 atgtttctgt tttctccact ttgccagaag acgaatggtc ctgcggcttc gcagaaattg
     6481 tgaagcattc ttttctggaa gaaacgtcgc gcgacctgaa ctatctggaa cagaacgtgc
     6541 aaaatattcg caaacccgag attctctctc ttgtgattcg cgacaatgtt agattcaaag
     6601 cgtccatagt ttcgcaggat gaaaaagaag cgggcctgag atcgatcctg aacctgggtc
     6661 acaccacagc acatgcgctg gagtctatca ctcactataa aaagttttcg cacggccagg
     6721 ctgtgtcgcg tggcctggtc acagcacttc tactttcaga gaaaacgggg ttgtctgcta
     6781 cggacagaga gcgcatgctt tccctgatgc agaagcttgc tcttcctctg gacactgctg
     6841 gattcggggc ggccgagctg cttgagcata tgaaattcga caaaaagaac acaggcgggc
     6901 agattcgttt tgttcttctt gaaaaaccag gcaagccagt attcgggatc ccaatcacag
     6961 cggaagaatt tgaaaacgcg tggtccgcac aaaaacggtt atttg
//
LOCUS       DAOQJL010000155         6958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6032_length_6958_cov_4.730697, whole genome shotgun sequence.
ACCESSION   DAOQJL010000155 DAOQJL010000000
VERSION     DAOQJL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_6958_cov_4.730697"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLG78_08525"
     CDS             <1..348
                     /locus_tag="PLG78_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23199.1"
                     /translation="VLEHFPDQKSAFESVSKMIRPGGIFAAALPSSHGPTLKYSPDDW
                     IRTHPEDHFADYSPRSLAKILPLYGMELINARPASFHPSRARGILRWPPLYRLYARAN
                     SFGDTMEILARKK"
     gene            complement(355..732)
                     /locus_tag="PLG78_08530"
     CDS             complement(355..732)
                     /locus_tag="PLG78_08530"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE23200.1"
                     /translation="MNSVAYFEIQSDNPAKSAEFYGSIFGWTCTHAPEMPIEYWRVET
                     AGIRGGLLKRPARIPPPQSGTNAFCCSMEVKNFDSVATQILDRGGQVALPKFAVPGVC
                     WQGYFIDPDGNTFGLFQADSNAK"
     gene            complement(776..2086)
                     /gene="hisD"
                     /locus_tag="PLG78_08535"
     CDS             complement(776..2086)
                     /gene="hisD"
                     /locus_tag="PLG78_08535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNE23201.1"
                     /translation="MPLRILDESSIDSASRARLIERAYAQDRSAFAAAQGAIDDVEVR
                     GADAVREFTKKFDGVDLSDILVSEEEWARGDLTASEVQDAFTKAYENILAFHRLQLDS
                     LKGARETIGGTVLGFRYQAVTTAAMYVPGGKASYPSSVLMGLIPAVVAGVKNRIVVTP
                     PDKDGTLLREILFCAKLAGATQIIKAGGSQGIAAAAFGHNAPGLRTPPAELIIGPGNR
                     YVTAAKSILASRGLVRIDSPAGPSEVLIIADHTARPDFVAADLLSQAEHGEDSAAVLC
                     CLSRKFAQEVSAEIERGFRERPARRDMKEKSIRDHSFAIVFESLENAVAFSNDFAPEH
                     LEICTENPEKVFEGIVSAGSVFLGHFAPVALGDYFSGTNHVLPTGRAAASYSGLGVDT
                     FLKRITYQHPTRESLKEALLPIQVMSKVEGLEHEHGHSVEIRFH"
     gene            complement(2067..3137)
                     /locus_tag="PLG78_08540"
     CDS             complement(2067..3137)
                     /locus_tag="PLG78_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23202.1"
                     /translation="MIVNKYLAFALLLLAAAPLFAGPDDYVEWKPIPGSSGYQLQVRD
                     AKTKRIMVDQFVEGTYLDVDLQVGHFEQRLAPLSPFGKPLIWSDWRPLNILVALSPVV
                     DEANAVEVKERRPFSMFLRGKNFTPNTRVFLKKDGNSIPVPNRSINAAGTELTLLVNP
                     ATIPPGNYSLYLENPRQKTWTGESLVSLKYAENAVAVVDPKDQKDVKNPLDQKDPRTQ
                     KNQRDQKDPKNQNKTQQNKSEVTGDFRDHLRTMPSTCKNSGLPDELVLKCYKRYIVLN
                     LSTPAKVELFNYLMVYQGNYDDRMRGYSAFRDSCNSMSRHTESLLRERLAQSGESLDL
                     AEKQQIQGTLSFIHNCNASSNP"
     gene            complement(3124..4974)
                     /locus_tag="PLG78_08545"
     CDS             complement(3124..4974)
                     /locus_tag="PLG78_08545"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE23203.1"
                     /translation="MDRLREIRIPLIVGSVAVMALLLFICDYTRRGDIGSREEIGSII
                     FRRHGVQRKFVDEVVWERIEQGTRLANSDSIRTDDKSEVKIRLKDGTVLTVDQNSMIQ
                     LDVASNRIRFETGSMQVQSSGSSSLTLQSGDSKVQMGEGSLTLSKSEKQDLSLTVYEG
                     TAKVTSGGKTENVGKNQKADIGKEGISVKEVPVVLIKPNPFQNVPAEGDGTSVSFKFQ
                     PSDSLKKPTLEISKDQEFKDSRKVQTPGVATMDLPPGTYYWRVTGEDAKTNQPAMSET
                     RKMTIVRGEPLRLHSPGSGERVAFNSNPPPVPFAWSQDPLAESYELEVARDAGFTQIV
                     KTIVTASNSVTIDDLPAGSYRWRIRTKPNQPDLNSRVSQSGSFEVYKTDLVPPETASP
                     FMSEHLTVSQAKEGIFFNWRAPGEMKSFVLQISQNPGFGNSLMNQQTEQNFSVVKGMP
                     EGIFYWRVRGTTASGKETPYSVVQSFTIGDKYMKSEIETPDATRVHVLFPLRQAVDMT
                     GRKSIDFKWEAVPGARKYNIRLYRNLGGKYEPIYAGSTTATGFSYTDMKSLDIGYFAW
                     EVQAEGGTLQPSGRIDFSITLNRYLQNLKPGDIQFVNPENIYREEQNDSK"
     gene            complement(4980..6809)
                     /locus_tag="PLG78_08550"
     CDS             complement(4980..6809)
                     /locus_tag="PLG78_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23204.1"
                     /translation="MLRSLLNRITNLVPEDDRGIRWTIRLKLSLIISLLVGLALFLII
                     SLASLFFRRDNEVRIQESSRQLAANVGTAVSAELKSRIQNLRLLSESLGAARGEKVLF
                     ENDRSLALVGIFQVQGDSIAPRTVLVNAESFKDSRGDIQQALVRLYPAFARARNGSIV
                     VRNVSPDLRVPLCAIAFPSRIQGGDRIIVMVLRLDELLAMFQTGGIIKSALVNEEGTV
                     IAHEDRDTVVSGTSFASVPIVKEMMASKLNNGLTEYDFKGVSYWGAFRRLDFGNVGVI
                     TYAEGSKWFEAVDRILKWNFLLMIVVICVAIIFVFFFARSFSEPILKLVGAASDIENG
                     RFILNLKPETSDEIGLLTKSFASMGRGLQERENLKVSFGRFVNKELAEMAMSGELDLG
                     GVSKEVTIFFSDIRGFTTISEKLEPEEVVEFLNQYMTEMVKCVQQTYGLVDKFIGDAV
                     MALWGALRPHGNDAVNAVNCAILMRKQLMKFNRGRGSARKPIIRIGMGINTGHVIAGQ
                     MGSAEKMDFTVIGDAVNTASRVEHLNKTYGTDILITDHTYEKTGKLFKVEEVDRVAVR
                     GKSKPVTLYAVLGMADDKDTPSNIKELRKMVGIEFNAAAAKKG"
     gene            complement(6886..6958)
                     /locus_tag="PLG78_08555"
     tRNA            complement(6886..6958)
                     /locus_tag="PLG78_08555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6923..6925),aa:Val,seq:tac)
BASE COUNT         1688 a         1814 c         1674 g         1782 t
ORIGIN      
        1 gttctggaac attttccgga ccagaagagt gcgtttgaat ctgtaagcaa aatgattcgc
       61 cccggtggaa tctttgccgc tgcccttccc tcaagccatg gacctacttt aaaatactcg
      121 ccggatgact ggattcgaac tcatccagaa gatcatttcg ctgactactc tcctcgcagt
      181 cttgcaaaga tactaccgtt atacggcatg gaactgataa acgcgcgacc agcgtctttt
      241 catccgtctc gcgcgcgcgg aatcttgcgc tggccaccac tctacaggct ctacgccaga
      301 gcaaattcct ttggagacac aatggaaatc ctggcgcgaa agaaataggt gcagttactt
      361 tgcgtttgag tccgcctgga acaaaccaaa cgtatttccg tctggatcga tgaagtatcc
      421 ctgccagcaa acacctggca ctgcgaattt tggaagtgcg acctgaccgc cccgatcgag
      481 aatttgagtg gccacagaat caaaattctt tacctccata gaacagcaga aagcattcgt
      541 tccgctctgc ggaggaggaa tccttgcggg tctttttaga agaccgccgc gaattccagc
      601 agtctctacg cgccagtact cgatgggcat ttctggcgcg tgcgtgcatg tccatccaaa
      661 gatagatcca taaaattcgg cagactttgc cgggttgtcg gattgtattt caaaataagc
      721 aactgagttc accgctcaag aatggagaca ttccagggag cgtcaattca aagagtcagt
      781 gaaagcgaat ctctacagag tgaccgtgct catgttcgag accttccact ttgctcatca
      841 cctgaatggg aaggagcgcc tctttcagac tttcacgcgt cggatgctgg tatgtaattc
      901 tcttcaagaa tgtgtccacg cccagaccgg aataggaagc agccgcgcga ccagtaggaa
      961 gaacatggtt ggttccacta aaataatccc cgagagcaac aggagcgaaa tgtccaagga
     1021 atacactgcc cgcagaaaca attccttcaa aaaccttctc tggattctcc gtgcaaatct
     1081 ccagatgctc cggcgcaaaa tcgttggaaa aagcgaccgc attttccagg ctttcaaata
     1141 caattgcgaa agaatgatcg cgaatgcttt tttccttcat gtcacgacga gccggacgtt
     1201 ctctaaatcc acgttcaatc tccgcactta cttcctgcgc gaatttgcga gaaagacagc
     1261 atagaacagc ggcgctatcc tctccgtgtt cagcctgact gagaagatct gcagcgacaa
     1321 aatcaggtcg agcagtgtga tcagcgatga tcaggacttc agagggaccg gccggcgaat
     1381 caatgcgaac gagcccgcga gaagctagaa ttgattttgc cgccgtgaca taccgatttc
     1441 ccggaccaat gatgagctct gcgggtggag tccgcagacc aggagcattg tgtccaaagg
     1501 ccgcggccgc aattccttga gaaccacccg ccttgatgat ttgcgtggcg ccggcaagct
     1561 tcgcacagaa aagaatttcg cgcaatagag ttccgtcttt gtcaggaggt gtaaccacaa
     1621 ttcgattctt cactccggct acgactgccg gaatcagacc catcaaaaca gaactgggat
     1681 aacttgcttt ccctccagga acatacatgg ccgctgtagt cacggcctga tatcgaaagc
     1741 caagtacagt gccgcctatc gtttcgcgag cacccttgag agagtccagc tgcagacgat
     1801 ggaatgcgag aatattctcg taagccttcg tgaaagcatc ttgcacctca gacgcggtga
     1861 ggtcccctct ggcccactct tcttcagata ctagaatatc cgaaagatct actccatcaa
     1921 acttctttgt aaattcgcgc actgcatctg caccgcgcac ttccacatcg tctatcgcac
     1981 cttgagctgc agcgaaagcg gaacgatcct gcgcgtatgc gcgctctatc agcctggccc
     2041 gcgaagcaga gtctatgctg gattcgtcaa ggattcgaag aggcattgca gttgtggatg
     2101 aacgaaagtg tgccctgaat ctgctgcttc tctgccagat cgagagattc gcccgattgt
     2161 gcgagccttt cccttaagag agattcagtg tgtctggaca tggagttgca actatcgcga
     2221 aacgcgctgt agccgcgcat gcgatcgtca tagttgccct gatagaccat caggtaattg
     2281 aacagttcta ccttagcggg tgttgataaa ttcaagacaa tgtatcgctt gtaacatttc
     2341 aaaacgagtt cgtcaggcag accggagttc ttgcatgtag acggcatggt ccgaagatga
     2401 tcacggaaat cccccgtgac ttcagattta ttctgctggg ttttgttctg attctttgga
     2461 tctttctgat ccctctgatt cttctgagtg cgcgggtctt tttgatccag cgggttcttt
     2521 acatccttct gatcttttgg atcgacgacc gccactgcat tctccgcgta cttcaaactg
     2581 acgagacttt ctccggtcca ggttttctgg cgcggattct caagataaag gctgtaattt
     2641 cctggaggga tggtggccgg attcacaaga agagtcaatt ctgtgccggc ggcgttgatg
     2701 ctgcgattgg gaactggaat gctgtttccg tctttcttca agaagacgcg cgtattgggc
     2761 gtaaagtttt ttccgcgcaa aaacatgctg aatggccttc tctcttttac ttccactgcg
     2821 tttgcttcat ccaccacagg ggaaagtgcc acgagaatgt tgagcgggcg ccagtcggac
     2881 cagatgagag gctttccaaa cgggctgagc ggtgccagac gctgctcgaa gtgacccacc
     2941 tggagatcta catcgagata tgttccctcc acaaattgat ccaccatgat gcgctttgtt
     3001 ttagcatcgc gcacctgcag ctgatatccg gaagaaccag ggataggctt ccactccaca
     3061 tagtcatcgg gacctgcgaa aagcggtgcc gccgccagga gcaaaagtgc aaatgctaag
     3121 tatttattta ctatcattct gctcttccct gtatatgttt tccggattca caaattggat
     3181 gtcgcctggc tttagattct gcaaatagcg attcaaagtg atagaaaagt caattcgacc
     3241 gctgggctga agcgtgccac cttccgcctg aacttcccat gcgaaatagc caatgtccag
     3301 cgacttcatg tccgtgtacg aaaaaccagt ggcagttgtg cttcccgcgt aaatgggctc
     3361 gtacttgccg cccaggtttc gatacaggcg aatgttgtac ttccgcgcgc ctggaaccgc
     3421 ttcccatttg aagtcaatgc tcttgcggcc agtcatgtcc actgcctggc gaagagggaa
     3481 aagaacatga acgcgagtcg cgtccggtgt ttctatttca ctcttcatgt acttgtcgcc
     3541 gattgtaaag ctttgcacta ctgaataggg agtctctttg ccagatgcag tggtgccccg
     3601 aacgcgccag taaaagattc cctctggcat tcctttgacc acggagaagt tttgctccgt
     3661 ctgctggttc atgagggaat tcccaaatcc agggttttga gaaatttgga gaacaaatga
     3721 tttcatttcc ccgggtgctc gccagttaaa gaagattcct tcttttgcct gagagactgt
     3781 cagatgttct gacatgaaag gactcgctgt ctccggcggg accagatcgg ttttgtaaac
     3841 ttcaaacgat ccgctctggc taacgcgcga attcaaatcc ggctggttag gcttcgtgcg
     3901 aattctccaa cgataacttc cagcaggaag atcgtctatc gttactgaat tagaagcagt
     3961 cacgatggtc ttcacaattt gagtgaatcc tgcatccctg gccacttcga gctcatatga
     4021 ttccgcaaga ggatcctgag accaggcgaa cggcacaggc ggaggattcg aattgaatgc
     4081 gacgcgttcc ccggaaccgg gagaatgaag gcgcaacggc tctccgcgca caatcgtcat
     4141 cttgcgcgtt tcactcatgg cgggctggtt agttttcgcg tcttcgcctg tcacgcgcca
     4201 gtagtatgtc cccggcggaa gatccatagt ggcgactcct ggggtctgca cctttcgtga
     4261 gtctttgaat tcctggtcct tgctaatttc aagagtaggc ttctttagag aatcggaagg
     4321 ctgaaacttg aaagatacag atgttccgtc cccttctgcc ggtacattct gaaatggatt
     4381 gggtttgatc aaaacgactg gcacttcttt gacggagatt ccttccttgc caatgtctgc
     4441 tttctgattc ttgcctacgt tctccgtctt gccgcctgac gttacttttg ctgtgccttc
     4501 gtagactgtg agactgagat cctgtttttc tgatttcgac agagtcaggc taccttcgcc
     4561 catctgcacc ttgctatctc ctgactgcag agtgagactg gatgagccgc tcgattgaac
     4621 ctgcatggat cctgtttcga atcgtatgcg attggatgca acatccagct ggatcattga
     4681 attctgatct acagtaagca cagttccgtc tttcaggcga attttgactt cagatttgtc
     4741 gtccgtgcga atgctgtccg aattggcaag cctggttccc tgctcgattc gttcccagac
     4801 tacttcatcc acgaatttgc gctgcacgcc gtgtctgcga aatatgatag atccgatttc
     4861 ttcgcgcgat ccaatgtcac cgcgacgagt gtagtcgcag ataaagagaa gcagcgccat
     4921 gactgccact gagcccacta tcagcgggat tctaatttcg cgtaatctgt ccatcgacct
     4981 cagcctttct tcgcagcggc cgcattgaat tcaatgccaa ccatcttgcg cagttctttg
     5041 atattagacg gggtatcttt gtcatccgcc atccccagaa ccgcataaag agttacaggt
     5101 ttgctttttc cgcgtactgc gacccgatcc acttcctcca ctttgaagag tttcccggtc
     5161 ttttcatacg tatgatctgt gatgagtatg tctgtgccgt aggtcttatt cagatgttcc
     5221 acacggcttg cggtgttcac tgcgtctcca atgaccgtga agtccatttt ctccgcagaa
     5281 cccatctgtc cagcaatcac atggcctgta ttgattccca ttccaatgcg aatgattggc
     5341 tttctggcag accctcgacc gcgattgaat ttcatgagtt gcttgcgcat gaggatggcg
     5401 caattgactg cattgactgc gtcgtttccg tgaggacgaa gagcgcccca caaagccatc
     5461 acagcatctc caatgaactt atccacgagc ccgtatgttt gctgtacgca cttcaccatt
     5521 tctgtcatgt actgattcag aaattcgacg acttcttccg gttctagttt ctcagaaatc
     5581 gtcgtgaaac cgcggatatc cgaaaaaaat atggttactt ctttgctcac tccaccaagg
     5641 tccagttccc cggacatggc catttctgcg agttccttgt tcacaaacct tccaaaagat
     5701 actttgagat tctcgcgttc ctgaaggccg cgacccatgg atgcaaacga ctttgtcaac
     5761 agtccaattt cgtctgaagt ttctggtttc agattcagaa tgaagcgacc gttttcaata
     5821 tcgctagcag cacccacaag cttcaagatt ggttcagaga agctgcgcgc gaaaaagaaa
     5881 acaaaaatga tcgcgacgca aatgactacg atcatgagaa ggaaattcca tttgaggatt
     5941 cgatccacag cttcaaacca cttgctgcct tctgcgtaag taatgactcc aacgtttcca
     6001 aagtcgagtc tgcggaatgc tccccagtaa ctaacgcctt tgaaatcgta ctctgtgagg
     6061 ccattgttga gtttacttgc catcatttct tttacgatgg gcacagacgc aaaagaggtt
     6121 ccggacacca ctgtgtctct gtcttcgtgc gcgatgacag ttccttcttc gttgacaaga
     6181 gcggacttga taatgccacc cgtttgaaac atcgcaagca actcatcgag acgcaatacc
     6241 atcacaatga ttctgtctcc accctgaatg cgagatggaa aagcaatggc gcataacggg
     6301 actcggagat caggactcac atttcgcact acgatggatc cattgcgagc tcgagcaaaa
     6361 gccggataca atcttacgag ggcctgctgg atatctcctc tggagtcctt aaacgattct
     6421 gcatttacaa gcacggttct gggcgcaata gaatcgccct gcacctggaa gattccaaca
     6481 agagcaagac tccgatcgtt ttcgaagaga actttctctc cgcgcgcagc accaaggctt
     6541 tcagaaagga gacgcagatt ctgaattctg gattttaatt ctgctgaaac cgcagtgccc
     6601 acgttggccg ccagctgacg gcttgattcc tggatacgaa cttcgttgtc gcggcgaaag
     6661 aaaagcgatg ccagcgagat aatcagaaaa agagcaagac ccacaagaag ggaaatgatc
     6721 aagctcaact tgagtcggat ggtccagcga atgccgcggt catcctccgg gacaaggttt
     6781 gtgattcgat tgagaaggct tctcagcatt ggagcaaagc tcacacggaa gcgcgaaacg
     6841 ccaatctttt attggaggaa gatgttttaa acgggagtag agatgtgggc gatgacagga
     6901 tcgaactgcc gacatcctgc ttgtaaggca ggcgctctac cagctgagct aatcgccc
//
LOCUS       DAOQJL010000156         6944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6053_length_6944_cov_3.523298, whole genome shotgun sequence.
ACCESSION   DAOQJL010000156 DAOQJL010000000
VERSION     DAOQJL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_6944_cov_3.523298"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1089
                     /locus_tag="PLG78_08560"
     CDS             142..1089
                     /locus_tag="PLG78_08560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE23205.1"
                     /translation="MAEPLVSVVMPSFNHARYILESIQSVLAQDFSDFELIVVDDAST
                     DNTREVLESIADDRVRFRINEHTGLPAITRNTGIAMARGEMVAFLDSDDLWEKNKLSS
                     QLEMLRAHPECGFCYHDFDTFDSETRKVLSRFGETCSLFEGDIAGAMLQGNMIGSPTP
                     LIQKSLLLRAGCFPEDWSVRFFEDWELWLRLAGLSPAAFVPAILAHYRIHSRPTFSGA
                     ALSLRFKTTLQVINRAVKDMPDRFKPHLPAALNAAYRIYGPTMLSQGATASFLKILFG
                     QFMVAGPIAHARAFQFALRARRVARRKLDSKLEVGSGQS"
     gene            1086..2282
                     /locus_tag="PLG78_08565"
     CDS             1086..2282
                     /locus_tag="PLG78_08565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE23206.1"
                     /translation="MRATKKRLRILFICPADVSTAQSFVATVADTGSFDVLVFDSGRQ
                     GPFPAQKWPFPTISIYRSEHPANGWHRWIGLPSNRFVRFLIQKRHWISALFLRMVIRV
                     YRPDVVHCLGINPESRILLRALRGISKTRRPPWALTSWSADVYWDRYKPECLSHLRES
                     FETVGWFLADCKRDLRIAVELGLSPAKLLIQDAIPGVGHVNAENIERPLREGPLTILV
                     PKAYDGVYHKPHIMLEALSRLRLRGLTFRLKIIGAQEDVRIWWRLRDPQGEAAAEFFD
                     SLPREEYLEMLRTADILLAPSLTDGTPNALLEAMSTGAFPIFSSHESIREWIEDGRNG
                     LLVDPVDCVDIEQKLIRVFENRALIPAAAELNGQIVKQRLSKVRMQELLTQGYETLAN
                     ATGGAG"
     gene            2279..3208
                     /locus_tag="PLG78_08570"
     CDS             2279..3208
                     /locus_tag="PLG78_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23207.1"
                     /translation="MKFLLFPIRLLRSIKELLRLVPLMSEEMERMRQAIGRVEARQVK
                     DATRIAEAEFRVYSQWGEDGIIQNLIGRIPGVDPSFIEFGVEDYRESNTRFLLVNNNW
                     RGLILDAAGSQLDTVRRSKTYWQHSLTAVTAFITRENINDLIQSAGFAGDIGLLSVDI
                     DGNDYWVWKAIDCVRPAIVVAEYNAILGCDHPLTIPYDSAFDRTRAHFSHVYYGASLS
                     ALHALGEEKGYALVASNSAGNNAFFVRRDLLGTLKALRPADVFVKPRFREARNPDGSL
                     GFLDLKAARDLIADMPLVNVSTGQVTNLRAAGV"
     gene            3210..4334
                     /locus_tag="PLG78_08575"
     CDS             3210..4334
                     /locus_tag="PLG78_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNE23208.1"
                     /translation="MPRILYDHQAFWIQKHGGVSEYFYHVIRRIRAMSSFSAELTAPR
                     TNNGYARELAGSEPFFRMSDRFFFRGAGRIDSVLRKIHVLPDYEKTNREMALSLLSSD
                     SFDVFHPTYYDDYFLANVKKPFVVTVFDMTHEMFPGFFPGQEIFLRQKKTLAERAAGI
                     IAISSTTRDDLCEAYGLDPKKVQVSHLGGPAPTPVARGRGSHILYVGQRSIYKNFSFF
                     LESIAPFLIKNEVRLIAAGGNPFTSTEKTLIHRLGVEKFVSHTRVSDDAAFIPLYRDA
                     LLFVFPSLYEGFGLPLLAAFAYGCPVACSNRGSLPEVAGDAAELFDPENSESILETVR
                     RVAESVSLQTALREKGMRRAGEFSWDRCASEHAKMYASLI"
     gene            4466..5287
                     /locus_tag="PLG78_08580"
     CDS             4466..5287
                     /locus_tag="PLG78_08580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE23209.1"
                     /translation="MPSSTEVAKLYSDYYPRRNYDPSTWHPPQDPRGFVSWLWGRKGG
                     AYAWVPERVRVLDVGTGAGESLGYHNVRGCQAVGMDPDANVERIAREKNVQIKIGLFS
                     NTDIQGESFDYVTMNQVIEHFVDPIEVMKEAARVLVPGGRLIMGTPNYRGLLARLFGA
                     RWIHWHIPYHIHFFSRQSMQKAASLAGLELESVNMVTESDWMFFQICHALTRPAPVSA
                     SRFWSVGEQRPSLTKLAMGLALIPRFLRLTHLWTRMLDAMGAGDNVVYICRKPLR"
     gene            5372..5971
                     /locus_tag="PLG78_08585"
     CDS             5372..5971
                     /locus_tag="PLG78_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE23210.1"
                     /translation="MSSFSRTFMDEVIDVSNKIDCDRIETIARGLAQVRENQGRVFFL
                     GVGGSAGNCGHAVNDFRKICGIECYAPTDNVSELTARTNDEGFEHIFSAWLKTSRLGP
                     KDAIFIFSVGGGNSEKNISVNLVHAIEFAKERGATIFGVVGKDGGHTGKSSSNVVIIP
                     TVNADHITPITEAYQAVVWHLLVTHPALKFNETKWESTK"
     gene            5953..6522
                     /locus_tag="PLG78_08590"
     CDS             5953..6522
                     /locus_tag="PLG78_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE23211.1"
                     /translation="MGVHKIERRAVFLDRDGIINRSIVRDGLPYPPASASELEFTPGI
                     ENTLRTLKEAGWFLVIVTNQPDVARGTATRESVEEIHSLIRHQLPVDAVYACYHDSGE
                     GCECRKPRPGMLLTAAREMNLDLSRSFMVGDRWRDVDAGNAAGCHTIFVDYGYTEELK
                     SRPDYVIDAPQKMTDIILQQKEEKGESNS"
     gene            6519..>6944
                     /locus_tag="PLG78_08595"
     CDS             6519..>6944
                     /locus_tag="PLG78_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNE23212.1"
                     /translation="MKKVSDLHIKIFADGAEKAGMLEMYKQPFISGFTTNPTLMNKVG
                     IKDYRAFAQDIVKAIPDRPISFEVFADDFAEMERQAGEIATWGEYVYIKDSSNQYESR
                     TFLRLDPPPHSEGSTVECDCGHDNRANGENCRSTQRNKGC"
BASE COUNT         1594 a         1714 c         1870 g         1766 t
ORIGIN      
        1 cttctgatcg ttttccaact ctagctcgcc gacctccaga tacttcgttt gataactccc
       61 tgttaaagaa gctcactggc agaaacccga cgactctgaa cgcggctctg gccgaaatga
      121 aatcgcaaat gaggcaatct ggtggctgag ccgctcgtat cggtggtgat gccgtcattc
      181 aaccacgcgc gatacatcct ggaatccata cagagcgttc tggctcagga tttttccgat
      241 tttgaactca ttgtggtgga tgatgcgtcc acggacaata cgcgcgaagt cctggaatct
      301 attgcagacg atcgcgttcg ctttcgaatc aatgagcata ctggactgcc cgcgatcacg
      361 cgcaatacag gcattgccat ggcgcgaggc gaaatggtcg cgtttctgga ttcagatgat
      421 ctgtgggaaa agaacaagct ctcctctcaa ctggagatgc ttcgcgccca tccggaatgt
      481 ggattctgtt accacgactt tgatactttc gatagcgaaa cgcgcaaagt cctgtccaga
      541 ttcggggaaa cctgctcact tttcgaaggc gacatcgcgg gagcgatgct gcaaggcaat
      601 atgattggtt cgccgacgcc tttgatccag aaaagtctgc tcttgcgtgc cggatgcttt
      661 cccgaagatt ggtccgttcg tttttttgag gactgggagt tgtggcttag gcttgcggga
      721 ctttcacctg ctgccttcgt tccggccata ctggctcact atagaattca cagtcgtccc
      781 actttttctg gcgcggcgct cagtctcagg ttcaagacga cgttgcaggt gatcaaccgg
      841 gcagtcaaag atatgccgga tcgtttcaag ccgcaccttc cggccgccct caatgccgcc
      901 tatcgtatct atggtcccac tatgctttcg cagggagcga cagcatcatt tttgaagatc
      961 ttgttcgggc agttcatggt tgccggtccg atcgcgcacg cacgagcgtt tcaatttgcg
     1021 ctcagggccc gccgtgttgc gcggcgtaag ctggattcga agttggaagt agggtcgggc
     1081 caatcatgag agccacaaag aagaggttgc gcatcctttt tatctgcccc gcggacgttt
     1141 ccaccgcgca gagttttgtc gcaacagttg cagatacggg cagcttcgat gtgcttgtgt
     1201 ttgattccgg tcgtcagggg ccttttccag ctcaaaagtg gcctttccca accatttcga
     1261 tctatcgatc ggagcatccg gcaaatggct ggcatcgctg gatcggtttg ccttccaatc
     1321 gctttgtgcg attcttgatt caaaaacgcc attggatctc cgcgcttttc ctgcgcatgg
     1381 tcattcgtgt ttatcggcca gatgtagtgc attgtcttgg aatcaatcca gagtcgcgca
     1441 ttttgcttcg cgcattgcgc ggcatttcaa agaccaggcg acctccctgg gcgcttacgt
     1501 cgtggagtgc ggacgtgtac tgggatcgat acaagccaga gtgtctgtcc catcttcgtg
     1561 aatcctttga gacagtgggc tggtttctgg ccgattgcaa acgcgatctt cgcatcgcgg
     1621 tggagctggg tctttcgcct gcaaaacttc tgatccagga tgcgattccg ggtgtgggtc
     1681 atgtaaacgc ggaaaacata gaacgacctt tgcgcgaagg tcctcttacc atccttgtcc
     1741 ccaaggcgta tgacggtgtt tatcacaagc cgcacatcat gctggaagct ctttcgcgac
     1801 tccgcctgcg cggcctgact tttagactga agatcattgg tgcgcaagaa gacgttcgca
     1861 tctggtggcg tcttcgcgat ccgcaaggag aggctgcggc tgaatttttt gattcactgc
     1921 cgcgcgaaga atatctggag atgcttcgga ccgccgatat tctcctggca cccagtctta
     1981 cagacgggac accgaatgct ttgcttgaag ccatgtccac gggtgcattt ccaatcttct
     2041 cgtcgcacga atcgattcgt gaatggattg aggacggccg caatggattg ctcgtggatc
     2101 cagtggactg cgtggacata gaacaaaagt taattcgtgt ttttgaaaat cgcgctctca
     2161 ttccggctgc tgcggagttg aatggacaga tcgtgaagca gcgcctgagt aaggttcgca
     2221 tgcaagaatt gcttacacag ggctatgaaa ctctcgcgaa tgctacgggt ggtgcaggat
     2281 gaagttcctt ttgtttccga taagattgct gcgttctatc aaagagctgt tgcgacttgt
     2341 tcctcttatg agcgaagaaa tggaacgcat gcggcaggcc atcggccgcg tagaagcacg
     2401 ccaggtgaag gatgcaaccc ggattgcgga ggcagagttt cgcgtgtatt cccaatgggg
     2461 cgaagatgga atcattcaaa atctgattgg ccgaattcca ggtgtggatc cgtccttcat
     2521 tgaatttggc gtggaagact acagagaatc gaacactcgc tttctgcttg tgaacaacaa
     2581 ctggcgaggt ctcattctgg acgctgccgg ttcgcagctc gacacggtgc gcagaagcaa
     2641 aacatactgg cagcatagcc tgaccgccgt gactgcattc attacacgcg aaaacataaa
     2701 cgatctgatc caatctgccg ggttcgcagg tgatatcggt ctgttgagtg tggatattga
     2761 tggcaacgac tactgggtct ggaaggcaat cgattgtgtg cgtccggcca ttgtggttgc
     2821 ggagtacaat gccattctcg gatgcgatca tccgcttacc attccgtacg attctgcttt
     2881 cgatcgcacg cgcgcgcatt tttctcacgt atactacggt gcgtctctga gcgcacttca
     2941 cgctctgggg gaagagaaag gctacgcact cgttgcatct aactccgccg gaaataatgc
     3001 attctttgtt cggcgcgatt tgctcggcac gttgaaagca ctgcgcccgg cggacgtgtt
     3061 cgtaaaacca cgttttcgcg aagctcgcaa tccggacggt tccctcggat ttcttgattt
     3121 gaaagcagcc cgtgatctca ttgcggacat gccgctcgtc aatgtttcaa ccggtcaggt
     3181 gacgaatctt cgcgctgccg gtgtctgaaa tgcctcgaat tctatacgat catcaggctt
     3241 tctggatcca gaagcatggc ggtgtctccg aatacttcta tcatgtaatc cgccgcatcc
     3301 gtgcgatgag ttccttttcc gcggaactca ctgccccaag aacaaacaat ggttatgcga
     3361 gggaacttgc tggctctgaa ccttttttca ggatgagtga tcgtttcttc tttcgcggag
     3421 ccgggcgaat agattcggtt ctgcgaaaga ttcatgtgct gccggactac gaaaaaacga
     3481 atcgtgagat ggcgctctct cttctttcgt cggattcttt tgacgtcttt catccaacat
     3541 actatgacga ctacttcctg gcgaatgtga aaaagccgtt cgttgttaca gtttttgata
     3601 tgactcatga aatgtttccg ggcttcttcc ccggccagga gatatttctc aggcagaaga
     3661 aaaccctggc agaacgggcg gccggaatca tagcgatttc ttcaacaacg cgtgacgacc
     3721 tgtgcgaagc ctacggcctc gatcctaaaa aagttcaagt ttcgcatctg ggtggcccgg
     3781 caccgactcc tgttgccaga ggcaggggga gtcatattct gtacgttggg caaagatcca
     3841 tctacaagaa cttctctttc tttctagagt caatcgcacc ctttctcatc aagaatgaag
     3901 tgcgattgat tgcagctgga ggaaacccgt tcacttcgac ggaaaagact ttgattcatc
     3961 gtctgggtgt ggagaaattt gtctcccaca ctcgcgtgtc ggatgacgca gcgttcatcc
     4021 ctctctacag agacgccctg ctgtttgtat ttccgagtct atacgaaggg tttgggcttc
     4081 cacttctggc cgcttttgca tatggttgtc ccgttgcctg tagcaatcgt ggctcgctgc
     4141 cggaagttgc gggagatgca gcggagcttt ttgatccaga aaattcagaa agcattctgg
     4201 aaaccgtgcg tcgcgttgca gaatccgttt ctcttcagac tgcactgcgc gaaaaaggta
     4261 tgcggcgcgc aggcgagttt tcctgggaca gatgtgcctc tgagcatgcg aaaatgtacg
     4321 cgagtttaat atgaatcctg tctgtcccat ttgctccgct caatctgtat tgcatcgttc
     4381 agatttgtac gacgatcgtt atgcgtatcc tggacggttc catgtgtatc gttgccagtc
     4441 ctgcgcgcat gcttttacaa atttcatgcc ttcttcgaca gaagtggcaa agctttattc
     4501 tgactactat cctcgacgca actacgatcc gtccacgtgg catcctccgc aagatccgcg
     4561 tggattcgtt tcgtggctct gggggagaaa gggcggtgcg tatgcctggg ttcccgagcg
     4621 cgttcgagtt ctagatgttg gcactggagc cggtgaatcg cttggatatc acaatgtccg
     4681 tggatgtcag gcagttggaa tggatccgga tgcaaacgta gagcgaattg ctcgcgagaa
     4741 gaatgttcag atcaagatag gacttttttc caacacggat atccagggag aaagttttga
     4801 ctatgtgacc atgaaccagg tgatcgagca cttcgtggat ccgattgaag tcatgaaaga
     4861 agcagcacgc gtactggttc ctggcggtag actgatcatg gggactccga attaccgtgg
     4921 tcttctcgcg cgtttgtttg gtgcacgctg gatccactgg cacattccat atcacatcca
     4981 tttcttttcc aggcaatcga tgcaaaaagc ggcatcactg gcgggcctcg agctcgaaag
     5041 tgtgaatatg gttactgagt ctgactggat gttctttcaa atttgccatg ccttgacccg
     5101 tccggctccc gtaagcgcgt cgcgattctg gtctgtcggg gagcagcggc cttcgcttac
     5161 gaagctggcc atggggctgg cattgattcc gcgctttttg cggctgactc atttgtggac
     5221 caggatgctc gatgcgatgg gagcgggtga caatgtcgtt tacatatgca gaaagccttt
     5281 gcgttgactc tattgcatct aaccactcgc agttcaaagt gattgcttgg acacgtcaat
     5341 tgcgcaaagg tggcaaaact cggagaaaga tatgagcagt ttcagtcgca catttatgga
     5401 cgaggtcatt gacgtctcaa acaagattga ttgcgatcga attgagacga ttgcgcgagg
     5461 actggcgcag gttcgcgaga atcaaggccg cgtcttcttt ctgggagttg gcggaagtgc
     5521 aggcaactgc ggtcatgcgg tcaatgactt tcgcaagatt tgcgggatag aatgctatgc
     5581 tcccacagac aatgtatcgg agctgactgc gcgtacaaac gacgagggtt ttgagcatat
     5641 cttctctgcc tggctcaaga catcgagact cggacctaaa gacgcaattt tcatattctc
     5701 tgtcggcggc ggcaattcag aaaaaaacat cagcgtcaat ctcgtgcatg cgattgaatt
     5761 tgcaaaagag agaggcgcga ctatcttcgg agtggtcgga aaagacggcg gacatacagg
     5821 caagtcttcc tcaaacgttg tgatcatccc gactgtgaat gcggaccaca tcactcctat
     5881 cacagaagcc tatcaggcag tggtgtggca tttgcttgtt acgcatccag ctttgaagtt
     5941 caacgagaca aagtgggagt ccacaaaata gaacgtcgcg cggtcttcct ggaccgcgac
     6001 ggaatcataa accgtagcat cgtgcgagac gggttgccgt atccgcctgc ctcggcgagt
     6061 gaacttgaat tcactccagg aatagaaaat actctgcgca cactcaagga ggctggctgg
     6121 tttctcgtga ttgttaccaa tcagcctgat gttgcgcgcg ggaccgccac cagagagagc
     6181 gtagaagaaa ttcactccct gatccgccat caattgccgg tggacgcagt gtacgcgtgc
     6241 tatcatgact caggggaagg ttgcgaatgt agaaaaccgc gtcccggtat gctactgacg
     6301 gcggcccgtg agatgaatct ggatctctca cgaagcttca tggtgggcga tcgatggcga
     6361 gatgtagatg ctggaaacgc ggcaggctgc catacgatct tcgtggacta tggctacact
     6421 gaagaactta aatccaggcc ggactacgtg attgacgcac ctcaaaaaat gacggacatt
     6481 attttacagc agaaggaaga aaaaggagaa agcaattcat gaagaaagta tcagatcttc
     6541 acattaagat tttcgcggat ggtgcagaaa aagcaggaat gcttgaaatg tacaagcagc
     6601 cgtttatcag cggattcaca accaatccta cgctcatgaa caaagtgggc atcaaagact
     6661 atcgcgcgtt tgcgcaggac atagtgaaag cgattccgga tcgtccaatt tcttttgaag
     6721 tttttgcaga tgattttgcg gagatggagc gtcaggcggg cgagattgct acctggggtg
     6781 agtacgttta catcaaagat tccagtaacc aatacgaaag cagaaccttc ctacgacttg
     6841 atccgccgcc tcactcagaa gggagcacgg ttgaatgtga ctgcggtcat gacaatcgcg
     6901 caaacggaga aaattgtaga agcactcaaa ggaacaaagg gtgc
//
LOCUS       DAOQJL010000157         6936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6065_length_6936_cov_3.577532, whole genome shotgun sequence.
ACCESSION   DAOQJL010000157 DAOQJL010000000
VERSION     DAOQJL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_6936_cov_3.577532"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1458
                     /locus_tag="PLG78_08600"
     CDS             49..1458
                     /locus_tag="PLG78_08600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE23213.1"
                     /translation="MLRYAAMILQKQEELSHMNKSRIAVIALVALIILCGAVRPDRLI
                     ALAAGAVSQNVCSGTFVSGLSPETVYDQEMRAEGGMSLINWALVYQVDQSERTVTTTV
                     FGRFKTRSRFHEGSGCRIEHPGEIPLGTLSKTAVVPHLPDIAGAEPVAPVNPKLAEAM
                     SEAFADNKNGAPRFTRAVVVVRQGRIVGERYSPGIGLDTPLISHSIAKSVLNAMIGIL
                     VRDGKMQIDQKVSASAWKTPVTVDQLLRMDGGLPVDEGRGPGLAQRMWFLERDQAAFA
                     QSTPLDTDPETKWAYSNLSYAVLSRLVRDATGGSPETVSSYMQRELFGPLGMRNATME
                     FDSTGSPMGSNAMFASARDWARFGLLYLNDGMAGKKRILPEGWVSYSKRRTLDSGYGA
                     GFWLNQTDSPIPGWGTPWGIPGAPRDAYMARGYLGQYIVIVPSESLVVVRLGVDRARG
                     SGIDGIGSLVQSVIAALHR"
     gene            1519..2037
                     /locus_tag="PLG78_08605"
     CDS             1519..2037
                     /locus_tag="PLG78_08605"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE23214.1"
                     /translation="MEVTDGRQFAVYMRTVRKRTLDVVQKVPIDRSQWRLSPDSMSVV
                     DIILHIASVEQALWGLDMATGTPGKLREFEKDSMNLSDAIATMANVRKGSSEFWSSLT
                     TTQLEQDILTPTGHSLPLKRWLVLAAEHEIHHRSFIHAYRKIWGLASSPIYGLTLDEL
                     RQHLEPKPASDS"
     gene            complement(2113..2502)
                     /locus_tag="PLG78_08610"
     CDS             complement(2113..2502)
                     /locus_tag="PLG78_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23215.1"
                     /translation="MNRNLYSILALVFAFSLYGQPGSGMGKNYDASKEVTVSGTVTEI
                     TRVPHGDMREGIHLAMSAGNETLVVMLGPTFYLEKQSVKVLKGDSITVIGSRTGNAII
                     ARQVTKGSQTLKLRTETGAPVWGRGGR"
     gene            2606..2869
                     /locus_tag="PLG78_08615"
     CDS             2606..2869
                     /locus_tag="PLG78_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23216.1"
                     /translation="MNYIKPLIAILVLSFARCGYEDIPNPRTGKTERQYQREKDQRNA
                     RKRIEFCTITVLVCQSMTPPGGTAPGGCNFTLSYVCTDYTNTI"
     gene            2969..3421
                     /locus_tag="PLG78_08620"
     CDS             2969..3421
                     /locus_tag="PLG78_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE23217.1"
                     /translation="MSNTGTVRLHRVLRAPPDRVYKAFVDKQALERWLPPYGFTGAIG
                     EMDVRVGSGYKMSFTNFSGGGSHSFSVKFVELVPAERIVHTDRFDDPNLPGEITVTIQ
                     LKKVSCGTDLSIVQEGIPAVIPEEMCYLGWQESLDQLTRLVEPEISAN"
     gene            3449..5632
                     /locus_tag="PLG78_08625"
     CDS             3449..5632
                     /locus_tag="PLG78_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23218.1"
                     /translation="MNHQKVERPGKIAFCVGWSIFSMLASVSSPVRAQSGLSIQTIPD
                     LSVMTSLDTTTQNSWWLATEPIDADRETKDAVAICNQKMIDRPALHWKQITVPGIIER
                     QTNFRGSTFWYRRLFRLSGAPEASVAVRLGRINDRDQTYLNGVLIGATGEWDSTIPAG
                     YDRTRIYGIPDNLLNRRGCNVLLIHVKRYFAESSGIATGVTVIGPASSITHEYFLEMI
                     RTSWMLGAYPALGLLFLIIYAVRRKQTEYLYFGLFTILLTLYELFRFDVKFELGISFF
                     ATKRIEYVLLYCLFPIFTAFLDTYFDAASLFRTRSIRRVILAANLYLICFILPTLLSN
                     DVDFWWTLQSHFIQPVWPVLFGYCLLILALQSMRGRKDALLLVPATLLLMAAVVLDIL
                     HAWNVVIHLPPLTIYAFSIFILSIAFLLAQRFAQSFSAVETTMSNLNATNAAYSRFVP
                     AEFLQILGKREITEVALGDQTLREMTILFSDIRSFTVMSEAMTPEENFNFLNSYLKRM
                     QPHVQMNGGFIDKYIGDAIMALFPRHPGEALDSAIAMQHEMIRFNQDRVAKGFQEIRA
                     GIGIHCGHLMLGTIGAADRMEGTVISDAVNLAARMEGLTKFYGAGILITEHVMMRLPD
                     PTRYLLRTLERVRVKGKSSAVSVIEVLDGYANCELYSASRPEFDRGMHSYLNREFKDA
                     ADSFHAVLDQNPGDGASKILLNRAIEHQNRGVGADWEGIIDMDAK"
     gene            5697..6443
                     /locus_tag="PLG78_08630"
     CDS             5697..6443
                     /locus_tag="PLG78_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE23219.1"
                     /translation="MRSEFALRALSPPGYHRANFMNESRKTSLTFGLFFAGTFVFSIP
                     ALFFYDPIFKNADYIRGGGFDPIISVGAIFEIFLAICNTATAIVIFPVTKRTSESGAL
                     AYVAVRIIESLIILTGVMSLMSIVTLRATATASQDPTVLVMMGKSLLAFHDWSFLLGP
                     QFCAGLGNGLVLGLLMLRSGLLPRPMALLGVIGGPLAFLGGVLVLFGILQPMSPGLFA
                     LTALEIIWEVSLTVYTLALGFKTIPSGQVA"
     gene            6457..>6936
                     /locus_tag="PLG78_08635"
     CDS             6457..>6936
                     /locus_tag="PLG78_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE23220.1"
                     /translation="MKRGKFQYYTATTIDGFIADENHSLDWLFEIQGGNSSFADFFAQ
                     LGAFVMGRSTFDWIVKHEDLLNQPEKWKQYHGDLPCWVFTHRPLPQVQGASIRAATGD
                     IASVCSEILAAANGKNVWLAGGGQLVGSFFDAGFLDELIFQIAPVTLGKGAPLLPRRI
                     "
BASE COUNT         1652 a         1809 c         1746 g         1729 t
ORIGIN      
        1 gcatctgatc actatcctct ccttgccagg ctgcgagtcg gttactagtt gctacgttat
       61 gcggcaatga ttctacaaaa gcaggaagag ttgagtcaca tgaataaatc cagaatcgca
      121 gtcatcgcat tggtcgcgtt gatcatcctt tgtggagcgg tacgaccaga tcgtttgatt
      181 gcacttgcgg caggtgcagt cagccagaat gtatgctcgg gcacttttgt ttcagggctc
      241 tcacccgaaa cagtctatga tcaggaaatg cgcgcggaag gcggcatgag tctgatcaac
      301 tgggcgcttg tttatcaagt ggatcaatcc gagcgcacag tgactaccac tgtgtttggc
      361 cgattcaaga caaggtctcg cttccatgaa ggatctggtt gccgcatcga acacccgggc
      421 gaaatccctc tgggcactct ttcgaagact gcggtcgttc cgcatctgcc tgacatcgcg
      481 ggtgccgaac ctgtggcccc ggtaaatccc aaactcgcag aggcaatgag cgaagccttc
      541 gcagacaaca aaaatggtgc acctcgtttc acgcgcgctg tcgttgtcgt tcgccagggc
      601 cgcatcgttg gtgaacggta ctctcctgga atcggactgg atactccgct catcagtcac
      661 tccatcgcga aatcagtgtt aaacgccatg attggaattc tggtgcgcga cggaaaaatg
      721 caaatcgatc agaaagtgag cgcaagtgcg tggaagactc cagtgacagt ggaccagctt
      781 ttgcgaatgg atggtggtct tcccgtcgat gaaggtcgcg gaccaggact cgcacaacgc
      841 atgtggtttc tagaaagaga tcaggctgct ttcgcacagt ccacaccact cgatacggat
      901 cccgaaacaa agtgggctta cagcaatcta agctatgctg tactttcgcg cctcgtgcgc
      961 gacgcgacag gaggatcgcc ggagacagtc tcctcctata tgcagcgcga actttttggc
     1021 ccgctcggaa tgcgaaatgc aacgatggag tttgacagta caggatctcc catgggatcc
     1081 aatgcgatgt ttgcaagtgc gcgcgattgg gctagatttg gtctgctcta tttaaatgac
     1141 ggcatggcgg gaaagaaaag aattcttccg gaaggctggg tctcttattc aaaacgccga
     1201 actctcgatt caggttatgg tgccggcttc tggttaaacc aaactgactc tccgattcca
     1261 ggatggggta caccgtgggg cattccgggt gcgccgcgtg atgcgtatat ggcccgcggc
     1321 tatctgggtc agtacattgt gattgttcct tctgaatctc tcgttgtagt gcgactcgga
     1381 gtggaccgcg cccggggttc cggcatcgac gggattggat cgcttgtgca gagtgtgatc
     1441 gccgcccttc atcggtgatc cattcgcaaa ccgcctggag tttctcgcat ggacgcagcc
     1501 gtagactaga gacaagaaat ggaagtgaca gatggcagac aattcgcagt ctacatgcgc
     1561 actgtgcgca agcgaactct ggatgtagta caaaaagtgc cgatcgatcg cagccagtgg
     1621 agactatcgc cagatagcat gtctgtggtg gatatcatcc ttcatattgc ttccgtggaa
     1681 caagcattat ggggattgga catggcaacc ggaacacccg gaaaactccg tgaatttgaa
     1741 aaagattcaa tgaatctttc agatgcgata gccaccatgg cgaatgtgcg caaaggatcc
     1801 tctgaattct ggtcctctct caccacgact caacttgaac aggacatcct gactccaacc
     1861 ggacattctc ttccgctcaa gcgctggctg gtgttggccg cagaacatga aattcaccat
     1921 cgttcgttta tccacgcata tcgcaaaata tggggattgg catcttctcc catctatggt
     1981 ctgacgttag acgaacttcg tcaacatctt gaacccaaac ccgcatccga ttcatgatct
     2041 ggccaatgac ctgctacgtg tgcaacgcag accctttgct ttctctacgg tccgcgttgc
     2101 acgcaatgac aattaacggc ccccacgccc ccagacaggc gcacctgttt ccgtgcgcaa
     2161 tttaagtgtc tgactacctt tcgtaacctg tcgagcgatg attgcattgc ctgttctgga
     2221 cccaatgacc gtaattgaat cacccttcag cactttgacc gattgcttct caaggtaaaa
     2281 ggttggtcca agcatcacca cgagcgtttc gttgcctgcg gacatggcaa gatgtatgcc
     2341 ctcccgcata tcgccgtgcg gtactctggt aatttctgtc actgtcccgg ataccgtcac
     2401 ttctttggac gcatcataat tcttgcccat gcccgatcct ggttgtccgt acagggaaaa
     2461 cgcaaacacc agtgcaagga ttgaatacag attcctgttc atcctaacct ccatgacttt
     2521 tcgtctttat aataaaagca ggaagaactg gcgccagccc cgagaaagat cagatattac
     2581 ccgtgataat tatcaggatt tcctcatgaa ctacatcaag cctctcatcg ccatcctggt
     2641 tctatcattc gcgcgctgtg gctacgaaga cattccaaat ccgcgaacag gaaaaacaga
     2701 gagacaatac cagcgagaaa aggatcagcg aaatgcgcgc aagcgaatcg agttctgcac
     2761 aatcacggtt ctggtctgtc agagcatgac acctccgggc ggcaccgctc cgggagggtg
     2821 caatttcacc ttgtcttatg tgtgcacgga ctatacaaac accatctaac gcaggcaaat
     2881 ctgactggac gaattgaatg acctgctcta acataacaca cgttagtcgc ccttgatcat
     2941 gcattcatgg cgctaaaagg agaaagaaat gtccaacacg ggaacagtcc gtctgcacag
     3001 agtactgcga gcaccacccg atcgcgtcta caaagcattt gtagacaaac aggcgctcga
     3061 gcgctggctt ccgccttacg gatttacggg agcgattggc gaaatggacg ttcgggtggg
     3121 tagcggatac aaaatgtctt tcaccaattt ttcgggtgga ggcagtcatt ctttctctgt
     3181 gaaatttgta gaactcgtgc cggcagagcg aattgtgcac acggatcgat ttgatgaccc
     3241 aaacctcccg ggtgaaatca cagtgaccat ccagctgaag aaggtttctt gcggaacgga
     3301 tctttctatt gtacaggaag gcataccagc agtgattccg gaagaaatgt gctatctcgg
     3361 atggcaggag tctctcgatc aactgactcg actcgtggaa ccggaaatat ccgcgaactg
     3421 atagcgccct ttgtttcaac agaactgaat gaatcaccaa aaggtagaaa gacccggcaa
     3481 gatagcgttc tgcgtgggat ggtcaatttt ctcaatgctt gcgtccgttt cctcccccgt
     3541 tcgagcgcag tctggattat ccatccagac cattcccgat ctttccgtga tgactagtct
     3601 cgacaccacg acgcagaatt cctggtggtt agcaaccgag cccatagatg cagatagaga
     3661 aaccaaggac gccgtggcca tctgcaatca gaaaatgatc gaccggcccg cgttgcactg
     3721 gaagcaaatc accgtgccag ggatcattga aagacaaacg aattttcggg gatccacatt
     3781 ctggtatcga agattatttc gtttgtccgg ggcgccggaa gcctctgtag cggtccgcct
     3841 cggcaggata aatgatcgag accaaaccta tttaaacggt gtattgatcg gggcaacagg
     3901 cgaatgggat tccacaattc cggcagggta tgacagaacg cggatatatg gcattccgga
     3961 taacctcttg aaccgcagag gctgcaatgt gcttttgatt cacgttaagc gctatttcgc
     4021 agaatcgtcg ggaatagcca cgggcgtcac cgtgattggt ccggcttctt ctataacgca
     4081 tgaatatttc ctggagatga ttcgcacatc ctggatgctc ggcgcctacc ctgccctggg
     4141 acttctcttt ctcatcatat atgcggtgcg acgcaaacaa accgaatacc tgtattttgg
     4201 gctatttacg attcttctca ccctttatga attgtttcga ttcgacgtga aattcgaatt
     4261 gggcatttct ttctttgcga caaagcgcat tgaatatgtg ttattgtatt gtttgtttcc
     4321 gatctttaca gcgttccttg atacctattt tgacgcggcc agtctgtttc gcacccggag
     4381 cattcgtcgc gtcattctag cagcgaattt gtatttgatc tgcttcattc tacccacgtt
     4441 gctttcgaac gacgtcgact tctggtggac cctgcaaagc cactttattc agccagtctg
     4501 gccagtttta tttggctatt gcctgctcat tctcgccctc cagagtatga gaggtcgaaa
     4561 agatgcgctt ctactcgttc ctgccactct gttgctaatg gcggcggtgg tcctcgatat
     4621 ccttcatgcc tggaacgtgg tcatccatct tccaccgtta accatatacg cctttagcat
     4681 atttattttg tcgattgcct tcctcctggc gcagcgtttt gcgcaatcat tttcggctgt
     4741 ggaaactacg atgagtaatc tgaacgcgac caacgcggcc tacagccgat ttgtcccggc
     4801 cgagttcctt cagatcctgg gcaagcgcga aatcacagaa gtggctcttg gcgatcaaac
     4861 actacgagag atgactatct tgttctcaga tattcgttcc ttcacagtga tgtccgaggc
     4921 catgacaccg gaagagaatt tcaacttcct caactcctac ctcaaacgaa tgcagccgca
     4981 tgtgcaaatg aacggtggtt tcatagacaa atatattggc gacgccatca tggcgctgtt
     5041 cccgcgacat cccggagaag cgcttgattc cgcaatagca atgcaacacg aaatgattcg
     5101 attcaatcaa gatcgagtcg ccaaaggatt ccaggaaatt cgggccggga ttggtattca
     5161 ttgcgggcat ttgatgctcg gcaccattgg cgctgcagat cgaatggaag gcaccgtcat
     5221 atcggacgca gtaaatctag cagctcgcat ggaaggactc accaagtttt acggagctgg
     5281 aatcttgatc acggaacacg taatgatgcg cctgcctgat cctacacggt atcttcttcg
     5341 taccctggaa cgcgtccgcg taaagggaaa gagctccgcg gtatccgtca ttgaagtgtt
     5401 ggacgggtat gctaactgcg agctgtattc tgcttcgcgt ccagaattcg atcgcgggat
     5461 gcactcctac ttaaatcgtg aatttaagga cgcagcggat tcatttcacg ctgtactcga
     5521 tcaaaatccg ggagatggcg caagtaaaat cctgctcaat cgagccatcg agcaccagaa
     5581 tcgtggcgtg ggagcagact gggaagggat cattgatatg gacgcaaaat gaaatcctga
     5641 gcatatcaga tggttgcgtg gaagtgaaga tcagtccgct ctagaaaaat tcccgaatgc
     5701 gaagcgaatt tgcattgcgc gcgctcagcc ccccggggta tcatcgcgcg aactttatga
     5761 atgaatctag aaagacctct ctcaccttcg gactgttttt cgccggcact ttcgttttct
     5821 ccatccctgc tctattcttc tacgatccaa tcttcaaaaa cgcagactac atccgcggcg
     5881 gggggtttga tcccatcatc tccgtcggtg ctattttcga aatctttctg gctatctgca
     5941 acacggccac ggccatcgtg atctttcctg ttacaaagcg cacgagtgaa tcgggcgctc
     6001 tggcgtacgt tgctgtgcga atcatagaat cgttgatcat tctcacagga gtcatgagcc
     6061 tcatgtcgat tgtaactctg cgggccactg caactgcgag tcaggacccg accgtgcttg
     6121 tgatgatggg aaagtctctc ctcgctttcc acgactggtc tttcctactt ggcccgcaat
     6181 tttgcgcagg tctgggcaac ggcctcgtgc ttggcttact gatgctgcga tccggtctcc
     6241 tccctcgccc catggcgctc ctcggtgtca ttggaggtcc gcttgcattc cttgggggcg
     6301 tgcttgtatt gtttgggatt cttcagccca tgagcccggg actctttgca ctcacggcac
     6361 ttgaaatcat ctgggaggtt tctctcacag tctatacgct ggctctgggt tttaaaacca
     6421 taccctctgg acaagtggcc taacaacggg tatgcaatga agcgaggaaa atttcaatac
     6481 tacaccgcga caacgatcga tggattcatc gcggacgaaa atcattcgct ggactggctc
     6541 ttcgaaatac agggaggcaa ttcgagcttc gcggacttct tcgcgcagtt gggcgcgttt
     6601 gtgatgggaa gatccacatt cgactggatt gtaaagcacg aagacttatt gaatcaacct
     6661 gagaaatgga agcaatacca cggagactta ccctgctggg tgttcactca caggccactg
     6721 ccacaggtgc aaggagcatc gattcgcgcg gcgaccggcg acattgcatc tgtctgctcc
     6781 gaaatactgg ccgcggccaa cgggaagaat gtctggctgg ccggaggcgg acagctcgtg
     6841 ggatcttttt ttgacgcagg ctttctagat gaactgatct ttcaaatcgc acccgtgact
     6901 ctgggcaagg gtgcgcctct cttacccaga agaatc
//
LOCUS       DAOQJL010000158         6906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6097_length_6906_cov_4.750985, whole genome shotgun sequence.
ACCESSION   DAOQJL010000158 DAOQJL010000000
VERSION     DAOQJL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6097_length_6906_cov_4.750985"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            744..2228
                     /locus_tag="PLG78_08640"
     CDS             744..2228
                     /locus_tag="PLG78_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNE23221.1"
                     /translation="MNPFKQIPVGRPIPESLHAVSLSLPTMAHVRGYEERLPSTVEHI
                     RSGYPRFVTHHLIEESKQHFLKKNRIEGKIAYCLNSQKAASGLLAFAGAGADVRTDRG
                     ITFVLFDAGSEAVKLAHSYLQHTGVCISSRCAEDYLIAEGVRPGTVQAAETDSKRVLE
                     ILGEAHKTSQSHVHLATSGMNAFFTVFRGLQEIQATKGRTLWLQLGWLYVDTGEIIKK
                     FTAPSDRAFMGDVFDMGAIRDFVSKNSDRIAGIVTECPTNPLVEMTDLQELHAITRSA
                     GAALILDPTLVTPLNVEVLVHSDAVINSLTKYASHMGDVMIGSAVLNPASPFYKELSR
                     KVETSIENPYPRDLARLAEQIENYHSVVAKINANTMRLATYLSNHKNIKKVYWAYAEQ
                     SRKNYEKFQRRAEAPGGIITIELNIPLEKFYDRITVAKGPSFGSVFTILCPFMYLAHY
                     DLVQSEAGRRELASLHLDPDLVRVSVGIEPIEEIIAAFAEALSD"
     gene            2221..3471
                     /locus_tag="PLG78_08645"
     CDS             2221..3471
                     /locus_tag="PLG78_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE23222.1"
                     /translation="MIDFGFDRFWKLTTAAGRLGWALLGDPTEQRRLKSAARAIESLS
                     KLKGAPMKLGQMLSLHEGLLPPEISGLLAGLRKDAEPIAVSEIQDILRLELGEEKASQ
                     LRLEEKPIAAASLGQVHRASHPDGRVFAIKVQYPGIREALGSDLRILRTIMTAFSPMT
                     AVPARKLIGEVKDRLLEEIDYVREASHLDQATEMYSDPIVIPTPVREFCTGRVLTTNF
                     IPSMSIAEASVSAETLRFQWAVHLLTAIVKGAVEHKFLHADPNAANVGFLSDGRIVLY
                     DFGCVKLVPESLSDSYSKAAAELLSGRLSPVPGLLFAAGIRYKSGEKLTEEFIQPIAR
                     MLNQVFPDEETVFGSIPDIYSKLWQMGTSDFLESRNIVFPPDLIFVHRTLLGHFGNLR
                     ALKPRGNWRQIVLGLLADAGVRPD"
     gene            complement(3494..4456)
                     /locus_tag="PLG78_08650"
     CDS             complement(3494..4456)
                     /locus_tag="PLG78_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE23223.1"
                     /translation="MMKKIVLIVLGLIVGSVVGLLGYVRFALPNVGPAPVMTIESTPE
                     RVERGKYLANHVTVCIDCHSTRDWSKFSGPLVPGAIGKGGELFDQKFGFPGAYYSRNI
                     TPAGIKNWTDGELFRLITTGVTKDGKAIFPVMPYPYYGRMDEEDIKSIIAYVRTLPAL
                     ENEVPASRSDFPMNFIVNTIPHKASLVKRPDPKDEVKTGEYLANAGACMECHTQVEKG
                     QVIPSLAYAGGREFPMPTGDMVRSANITPDVETGIGKMTRKEFVARFKERMAAARAPA
                     QGFNTIMPWGMYGGMTDQDLGAIYEYLRTISPKANNVVKYTPAK"
     gene            complement(4482..5960)
                     /locus_tag="PLG78_08655"
     CDS             complement(4482..5960)
                     /locus_tag="PLG78_08655"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNE23224.1"
                     /translation="MSDLNGVMMQYFQWYSPADGSYWKQTAANARELAAAGITALWLP
                     PAYKGIGGGADVGYGVYDMYDLGEFNQKGSVRTKYGTKEEYLSCIQTLQQSGIQVYAD
                     TVLNHRMGGDNPETPVATPFWQDNRMYAKGEPRKIQAYTHFQFPGRAKKYSDFEWHWN
                     HFDAVDYDGFNPNDHSTVYLLEGKQFDDRVAMEKGNFAYLMGCDLDCQSADVRAELIR
                     WGKWYLDTTKVDGFRLDAIKHIAAWFFPEWLDTLEKYVNKDLFVVGEYWVPDVEALHN
                     YLNLVGERMTVFDVPLHFNFHAASKSGGNFDMRRLFSGTLMEQRPRMAVTFVDNHDSQ
                     PLQALESCIEPWFKPLAYAAILLRREGYPCIFHPDYYGADYEDRGKDGNTYRIVMPSH
                     RFLIDKFLYARRNFAYGPQYDYFDHYNSIGWTRLGDLSHPKSMAVLMSDGPAGHKWME
                     TGKRGAQFRDITGHVSHIVTTNGDGWGEFHCNGGSVSVWVEE"
     gene            complement(6012..>6906)
                     /locus_tag="PLG78_08660"
     CDS             complement(6012..>6906)
                     /locus_tag="PLG78_08660"
                     /inference="COORDINATES: protein motif:HMM:NF023145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HNE23225.1"
                     /translation="SYLANYDIDSGSPESTGVNIRRIAEILASSFKGKMVIFTGDFCY
                     SGAFEEALRTLQKKGIRGFMLASASASNESTGNWTFSQTWIDCLSGNPLCDKNGDGKI
                     TLGEAQSEVAQAMKFRERQRNGFLLAGVSESLIISSATGKNQPGVGSYVLAPRAGAML
                     PARITNRNGSRVTCEFYSYSKKTSVDLAENAVKPLNFRQYSVGTNLKVIWNGKSYPAT
                     VKKLDNDFHLITYSGYDSSWDEWVMDDRIVADDSVLVEWKGKWYPAKVLKTEGGRSFI
                     HYEGFESSWDEWVTSSRIKKP"
BASE COUNT         1735 a         1776 c         1686 g         1709 t
ORIGIN      
        1 gccaggatca aactcttcgt tatgtaatcc gtattgctac ggactgcaat ttgtgatttt
       61 ttgcaccgat tactcggtgc gtccgtttcc ggacagccat cgcaactttc gctgcttcaa
      121 gcatcactca tgatcatgac tgactaagat actgtgttcg agaatttctt ctcgtataaa
      181 gatctatgct ccattcagat tgaacggagt ccgcatctat tcagctgtta aagatccaga
      241 tcacgtgaat tgctttcgca attcgcgccc cggttttgcc gggtgagggg ccactgtttt
      301 ccaaccgact caccgtgtca aacacaaatg ggaaaaattt tcccatcccc ttcgaaaaaa
      361 ttttcgaagg tgcgaatcct ttttttggac agccctcgct ggtaattcat agtattgcac
      421 acaaacaaaa gatgatcaag aaaaatctga cataaatcca ggaattttcc gcgcgaaaac
      481 gaatccctgg tgcttcacca ggggattttt tgaagatttt tctaatgaga cataatctag
      541 aaaaggggga catgagcaat gtcgcctaac gtgtcgcgaa agagtacgtt cgcattgcac
      601 attcgacaaa tcctgaaata catttctaca tccgcacaaa gacttgaaat actgatttat
      661 gcgctaaggc acacgaaggc caccgaggcg atcgccattg caaatgactt gagcgcctga
      721 agcctcgaaa gattgtgtta gagttgaatc cttttaaaca aatacctgtg ggaaggccga
      781 tccctgaaag tctgcacgct gtttcgctca gcctgcctac gatggctcac gttcggggat
      841 atgaagagag actcccctct actgtggaac acattcgatc ggggtatcca cgatttgtga
      901 cccatcatct tatagaagaa agcaagcaac acttcttaaa gaagaaccgc attgagggga
      961 agatagcata ttgcctgaat tcacaaaaag cggcaagcgg cctgctcgcg tttgcggggg
     1021 ccggggcgga tgtgcggacc gaccgtggaa tcacctttgt gttgttcgac gccggatcag
     1081 aagcagtcaa actcgcgcac agctatctcc agcataccgg cgtgtgcatt tcttcgcgat
     1141 gcgcggaaga ctacctgatc gcagagggag ttcgccctgg aacagtgcag gcggcagaga
     1201 ccgatagcaa gcgtgtcctt gagattctgg gggaggcgca caagacttcg cagtcccacg
     1261 tccatcttgc aacgagcgga atgaacgcgt tcttcacagt gttccgcggt cttcaggaaa
     1321 ttcaggccac aaagggccgc actctctggt tgcagctcgg atggctctac gtagatacgg
     1381 gagaaatcat caagaaattc actgctccgt cagatcgcgc attcatgggc gacgttttcg
     1441 acatgggcgc gatacgcgat tttgtttcaa aaaactcaga caggatcgca gggatcgtca
     1501 cagaatgccc gaccaacccg ctcgttgaga tgacagatct ccaggaattg cacgcaatta
     1561 ccaggtccgc aggcgcggcg ctgatcctgg atccaactct ggtcacgcct ctgaacgttg
     1621 aagttctggt gcattctgac gccgtcatca acagccttac gaaatatgcc tctcatatgg
     1681 gagacgtgat gatcggctct gctgttctaa accctgcatc tccgttctac aaagagcttt
     1741 cgcgcaaagt agaaacatcc attgagaatc cctacccgcg cgatcttgcg cgcctcgcag
     1801 aacagatcga gaactatcac tctgtagtcg cgaaaatcaa tgcaaatacg atgcgactgg
     1861 caacatacct ctcaaatcac aaaaacatta agaaagtata ctgggcctat gcagaacagt
     1921 cgcgcaagaa ctatgagaag tttcagcgca gagcggaggc gccgggcggg atcattacga
     1981 ttgaactcaa tattccgctc gagaaattct atgatcgcat aacagttgct aaaggcccga
     2041 gttttggttc tgtttttacg attctttgcc cgttcatgta tcttgcccat tacgatctgg
     2101 tccagtcgga ggcgggaaga cgcgagcttg ccagtctaca cctggatccg gacctggttc
     2161 gtgtttctgt tggaatagag ccaatcgaag aaatcatcgc agctttcgcg gaagcgctca
     2221 gtgattgatt tcggttttga ccgattctgg aaactcacga ctgccgccgg tcgactcggg
     2281 tgggcattgc ttggcgatcc gaccgagcaa cggcgtttga aatcggcggc gcgcgccatc
     2341 gaaagcctga gcaaactgaa aggggcgccg atgaaactcg ggcaaatgct ttctctgcat
     2401 gaaggactgc tgcctccaga aatctccgga ctgctggccg gactgcggaa ggatgcagaa
     2461 ccaattgctg tttctgaaat tcaagatata ctgaggctgg agctgggcga agaaaaggca
     2521 tcgcaactca ggctggaaga aaagccaatc gccgcggcgt ctcttggcca ggtacatcgc
     2581 gcttcgcatc cagatggtag ggtgttcgca atcaaagtgc aatacccggg aatacgcgaa
     2641 gctctgggaa gtgatctaag aatacttcgg acgatcatga ctgctttttc cccgatgact
     2701 gccgtgcctg cgcgaaagct gattggtgag gtgaaggata ggcttcttga ggagatcgac
     2761 tacgtacgcg aagcctctca cctggatcag gcaacagaga tgtattcaga tccaattgtg
     2821 atcccgacgc cagtaagaga attctgcaca ggccgcgttc tcaccacgaa ctttattccg
     2881 tccatgagta ttgcagaagc gtccgtgtct gcggagactc ttcgattcca gtgggccgta
     2941 catctcctta ctgcgatcgt gaagggggcg gtagaacata aattcttgca cgcggatcca
     3001 aacgcggcta acgtaggatt tctttccgac gggcgcattg tgctttatga ctttggctgt
     3061 gtcaaactgg tgccggaatc tctttcagac agctactcaa aggccgccgc ggaactcttg
     3121 agcggcagac tttcgcccgt acccggtctt ttgtttgcag cggggattcg ctataagagc
     3181 ggagaaaaac ttaccgaaga atttatccag ccaatcgcaa gaatgctaaa tcaagtattt
     3241 ccggacgagg agactgtctt tggatccata ccggatatct attcaaaact atggcaaatg
     3301 ggtacttcag actttcttga atcaagaaac attgtgtttc cacccgactt gatctttgtg
     3361 catagaacgc tgctcggtca ttttggaaat cttcgagcgc tgaaaccacg aggcaactgg
     3421 agacaaattg tgcttggttt gcttgctgac gccggcgtcc ggcctgattg accggagccg
     3481 tgcagtataa gaactatttt gccggagtgt atttcacaac gttattcgcc tttggcgaaa
     3541 tggtgcgaag atattcgtag atggctccca gatcctgatc tgtcatgccg ccatacatgc
     3601 cccacggcat aattgtatta aaaccttgag caggtgcgcg ggccgcggcc atgcgctctt
     3661 tgaatcgcgc gacgaattcc tttctggtca ttttgccaat tccagtttcc acatcaggag
     3721 taatgttggc agaacgaacc atatcgccgg taggcattgg aaactcgcga ccacctgcgt
     3781 aagcaagaga tggaatcacc tgcccttttt ccacttgagt gtgacattcc atacaagctc
     3841 cggcatttgc caggtattcg cctgtcttca cttcatcctt aggatcaggg cgcttcacga
     3901 gacttgcttt gtgtggaatt gtatttacaa tgaaattcat tgggaagtcc gacctggaag
     3961 cgggcacttc attttccagg gcaggcagag tacgcacgta cgcgatgatg cttttgatgt
     4021 cttcttcgtc catgcgaccg tagtacggat aaggcatcac ggggaagatt gccttaccat
     4081 cttttgtaac gcctgtggtg atcagccgaa acaattcgcc atctgtccag ttcttgattc
     4141 cagcgggagt gatgttgcgc gaatagtacg cgccaggaaa accaaacttt tgatcgaata
     4201 attctccgcc ttttccaatg gcaccaggta caagcgggcc tgaaaattta gaccagtcgc
     4261 gagtggaatg gcaatcgatg catacagtca cgtggtttgc cagatacttt cctcgctcga
     4321 ctctctccgg tgtagattca atggtcatca cgggtgccgg gcccacgttg ggcaaagcaa
     4381 aacgcacata accgagcagg cccactactg atccaacgat caatccgagc acaatcagaa
     4441 ctatcttctt catcattccc ccttttcaat gccgtctaac gttactcttc tacccagacc
     4501 gagacggatc caccattaca gtgaaactcg ccccagccat ctccatttgt ggtcacaatg
     4561 tgcgaaacat gtccggtaat atcacgaaac tgcgctccgc gttttccggt ttccatccat
     4621 ttgtggccgg ccggaccatc gctcatgaga actgccatac tcttcggatg cgaaaggtcc
     4681 ccgagtctgg tccaaccaat tgaattgtag tgatcaaagt aatcgtattg cggtccgtaa
     4741 gcgaagttgc gccttgcata tagaaatttg tcaatgagaa agcgatgcga aggcatcaca
     4801 atgcgatacg tattgccgtc tttgcctcga tcttcgtagt ctgcgccata gtagtcagga
     4861 tgaaagatgc atggatagcc ttcgcggcga agcaaaattg cggcataggc gagaggctta
     4921 aaccagggct caatgcagga ctcaagggcc tgaagaggct gcgaatcatg attgtctaca
     4981 aacgtaaccg ccatccgtgg acgctgctcc atgagagtgc cggagaaaag gcggcgcata
     5041 tcaaaattgc caccggattt actggccgcg tgaaaattga aatgaagggg aacgtcgaaa
     5101 acagtcattc gctctccgac cagattcaga tagttgtgaa gcgcctctac gtctggcacc
     5161 cagtactcac ccacaacgaa caggtctttg tttacatact tctcaagagt atccagccac
     5221 tccggaaaga accaggctgc aatgtgcttg atcgcatcca ggcgaaaccc gtccaccttc
     5281 gtagtgtcca gataccattt gccccatcga atcaattctg cacgaacgtc cgcactctgg
     5341 cagtccaggt cgcatcccat gaggtacgcg aagtttcctt tttccatagc aacgcgatca
     5401 tcaaactgtt tgccttccaa aagataaact gtggaatgat cgttgggatt gaagccgtca
     5461 taatctacag cgtcaaagtg attccagtgc cattcgaaat cggagtattt cttcgctctg
     5521 ccgggaaatt gaaagtgtgt gtatgcctgg atcttgcgtg gctctccttt cgcgtacatc
     5581 ctgttatcct gccagaatgg agtggccact ggagtttcag gattgtcacc acccatgcga
     5641 tgatttagta ctgtgtccgc atatacctgg atgcctgact gctgcagtgt ctggatgcat
     5701 gagagatact cttcttttgt tccgtacttt gtgcgaacgg atcccttctg gttgaattcg
     5761 cccaggtcat acatatcgta gacgccgtag ccaacgtccg cccccccacc gattcccttg
     5821 tatgcgggag gaagccagag agcggtaatt ccagcagccg caagctcacg agcattggca
     5881 gcagtttgct tccaataact gccatccgcc gggctatacc actggaagta ttgcatcata
     5941 acgccgttca agtctgacat gaagattctc cttgggcatt gaggaacaaa gctttagaaa
     6001 tgtaaactgt ttcagggttt ctttatgcgc gaagacgtca cccattcatc ccaggaagac
     6061 tcaaatccct cgtaatggat aaacgatctc cctccttctg ttttcaaaac cttcgcagga
     6121 taccacttcc ctttccattc caccagcaca gaatcatctg cgacaatgcg atcgtccatg
     6181 acccattcat cccacgacga atcgtatccg gaatacgtaa tcaaatgaaa atcgttgtcc
     6241 agcttcttga cagtggccgg gtaagatttt ccattccaga tgactttcaa attggttccg
     6301 acagaatact gacgaaagtt taaaggtttc accgcattct ctgcgagatc cacagaagtc
     6361 tttttgctat aggaatagaa ttcgcaagtt accctggatc cgtttctgtt cgtgatacga
     6421 gccggaagca tggcacccgc gcgcggagcg agcacatagg agcctactcc gggttgattt
     6481 tttcctgtcg cagacgaaat gatgagagat tcgctgacac cggcaagcaa gaagccattt
     6541 ctctgtctct cacgaaactt catagcctgc gccacttctg actgagcttc tcccagagtg
     6601 atttttccgt caccgttctt gtcgcataac gggttgccgg ataaacaatc aatccacgtc
     6661 tgcgaaaaag tccagttgcc tgtggactca ttggacgcgc tggccgacgc aagcatgaaa
     6721 ccgcgaattc ctttcttctg cagagtgcga agagcttctt cgaacgcacc tgaatagcag
     6781 aagtctcctg tgaagatcac catcttccct ttgaagctgg aggccaggat ctccgcgatg
     6841 cgacgtatgt ttacgcctgt agattcgggg ctgccagaat caatatcgta gttggccagg
     6901 taggac
//
LOCUS       DAOQJL010000159         6854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6162_length_6854_cov_4.783792, whole genome shotgun sequence.
ACCESSION   DAOQJL010000159 DAOQJL010000000
VERSION     DAOQJL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_6854_cov_4.783792"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /locus_tag="PLG78_08665"
     CDS             <1..2296
                     /locus_tag="PLG78_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE23226.1"
                     /translation="FFPMEFISPGKAVSHSFIAVPGSWNKAPYNGDSLGAHAFGSFRM
                     RILVQTDRPLAFRVGEVGTAYRLICEGQLLGGRGFPSDNPEISVPLTQPATYIFTPHS
                     NQVEVILHVSNFHYRKGGIWHSISFSDANRITDLIVLKQWTSIVVFAMLAILGMYHVA
                     FWFVGRRSFFFFGLVCIDIALRETVTGSKIALLVIPALPWELSIKVEYWTLTWSSGFF
                     ALYMNSLFPAFFHRSWTRGMVGLSVLVSIMVLFTPASFYTQTVYLQNGYTALTVLLSV
                     IAATRARMAGFTSATAVLLPGMVLSISVINDILHTNHFINTGNFTSVGLTVFVFGQAV
                     LLGNQFTRSFSQTRTLGRKLRAQKRTLSHFNTRLEEEVKTRTSELELARHKAESAANA
                     KTMFLANMSHELRTPLSSIIGFSELLQREGLNPEESANYMRIVAGNARHLLQLVNEVL
                     DYSKLETGAMRFEKVDFRLRDMILGVIDSESIKAASRGDIVEIEIDAAVPSIVRGDPT
                     RIAQVLYNLVNNAVKFTDNGVIRVEIKALSQDAQTVDLQFSITDTGIGIAQEQRSIIF
                     DRFGQADSGIARKYGGTGLGLAIVQMILQALGSSLQLESSPGNGSRFYFHLKFEKGGE
                     AATILEAPVSNERLAGIRVLVVDDNEINRMLAEKLLQRWGATVDGAADGEEGVRKAQS
                     SAYDVILMDLQMPGMDGYEAARRIRDLGIKTPLIALSAAALDEVKDRALEAGMDDFVS
                     KPFHQEELYSRLVQHTSPDVRKNH"
     gene            complement(2270..3706)
                     /locus_tag="PLG78_08670"
     CDS             complement(2270..3706)
                     /locus_tag="PLG78_08670"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE23227.1"
                     /translation="MRDLKFEVDLFLAKLQLINEKGTGQPSVDKKRIKENLHEFLRDD
                     MIYFLQKLLSEHRTTFEKMMPGLVPSHREIIFQLKELQARVFYPVGKDFSKTSMIGRW
                     LLALHARGHLSPHKVMNYFNIYKNRLYSLLEEKPFGFSDTETLVRRMLFSTDYEVSFD
                     EIYSAEIELKREITFTLLRRVSTQSRIDYLVSKEVVEELSDNFIRILHRETAARLKQA
                     LAEMDELNKRILAEKKTVESEIKIAAKSQDKNLQKSLPADDPRIKFALWYDPLMSVGG
                     DYYNVIRVSEHEYAIFLADISGHGIGAAMFFNTLRLAFEESLESADRPEKTMKLVNES
                     VYGKLSDNFVTAIYVYVNLKKKIIKYCNAGHPKAFLFQKDGDRMRVRFLRQTGRVLGL
                     FRKVSYKEKQIPLGGTARLIVYTDGITEAYGRENQMLGERGFLSMFRHTLTSGAEDAM
                     ESIRKDVLAFSGTEQREDDRSVILADIG"
     gene            3839..4663
                     /gene="uppP"
                     /locus_tag="PLG78_08675"
     CDS             3839..4663
                     /gene="uppP"
                     /locus_tag="PLG78_08675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNE23228.1"
                     /translation="MIQVTEAAILGIIQGLTEFIPISSTAHLRIIPAMLGMGDPGAAY
                     TAVIQLGTLFSLIIYFWRDLLNFAQSSLQGLASGSPFRDENARMVWYLGVGTIPVCVF
                     GLLFAHKIKGDFRSLYVIAGSLIVFAITLYIADRVSRRHKTIHQFDLRDALLIGLAQS
                     VALIPGASRSGMTLTMALLLGYTRESGMRISFLLSIPAIAISGLYELYEERRALAEAG
                     FVGLAVATIVSGIVGYMSVAFLLRYLKTHSTTAFVLYRIAMGVVILGLLRAGYLMS"
     gene            4761..>6854
                     /locus_tag="PLG78_08680"
     CDS             4761..>6854
                     /locus_tag="PLG78_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtN system VIT domain-containing protein"
                     /protein_id="HNE23229.1"
                     /translation="MSENRIQKYFIRPVRIVGMELPAWGWMVGSFAAICFLAVCVYAL
                     LYANVLRWLNSSILLWGSGVFLGIVFLGLFLVLLRAVFSSIRQFTIFTLVLAAASFAT
                     NAYVLSHEVFPPFTSRTEVALWVCFIALAISALNGWLSDPIRYALLPLLSAGAFLCVA
                     FVIVLGPLMAFAWFFVWAAGLGFLPYSPLFALIAFVLNLMRLESSVRQRVVARWLIAG
                     PAALIVIYSIYYKIQWNRAEEVMRRPEISAGHNRLDEDLPLWIQKAARIPANHVTQMV
                     LQPNRRSEISIFGLQTLFDPLAFLSSTSPWSGSRGVDIISAEDSGRMLHLLYGQSHAY
                     LDRLWRGHSLITTNVQTHVQIHPELRVAYTETTLSIFNEDQDSNHKVMGLRLSAGNVP
                     QEAIYTISVPEGSICTKLSLWVNGEERPARLTFKSTARRAYETIVSVERRDPSYVEWL
                     DGNRLRLRVFPVSPGQYRTVRVGIVSPLRADEGRLTYNRIEFDGPVQTFAEQSVLVDL
                     FSSARLDVESSGMFLKEKVVSDGQVRQFRGSLVRPHWSFSFPAPAAGGSISAGGKSFL
                     VHPLQMENKPFSPQAILVAVNGSRSRAAWKEFISKTYGLGLPVYILTNEWFETADVKK
                     AMAYLDDCEIPAFNLYPPAFPATRKLGPVLWVTSGEEQSIPLGELRGSIRFANTQIAA
                     TERPADAVAVLNGRVS"
BASE COUNT         1502 a         1658 c         1747 g         1947 t
ORIGIN      
        1 gttctttcct atggaattca tcagcccggg taaggcagtc agccattctt tcatcgcggt
       61 tcccggatca tggaacaagg ctccgtacaa tggcgactct ctcggtgcac atgcttttgg
      121 ctcttttcgc atgcgaattc tggttcaaac agatcggcct ctcgcgttta gagtaggcga
      181 agtcggcacc gcctacaggt tgatatgcga aggtcagctc ctgggaggtc gcggttttcc
      241 atctgacaat ccagagatat ccgttcctct gactcagccg gccacgtaca tcttcactcc
      301 tcattcaaat caggtggaag tgattttgca tgtttccaat tttcactatc gcaagggtgg
      361 aatctggcat agcatttcgt tttccgacgc aaacagaatc acagacctga tcgtgctgaa
      421 gcaatggacc agcattgtag tatttgcaat gctcgcgatt ctagggatgt atcacgttgc
      481 cttctggttt gtcggaaggc gaagcttctt cttctttggt ttggtctgca ttgatattgc
      541 cctacgggag acagtgactg gaagcaaaat cgcgttgctt gtgattccgg cgctgccgtg
      601 ggaattatcc atcaaagtcg aatactggac tttgacctgg tcttccggat tcttcgcact
      661 ctatatgaac tccctttttc ctgcgttctt tcatcgcagc tggacgagag gcatggttgg
      721 tctttccgtt cttgtgagca tcatggtgct tttcactccg gccagctttt atacgcagac
      781 tgtctatctg caaaacggat acactgccct cactgtctta ctgtccgtga ttgcagccac
      841 tcgtgcgcgc atggccggtt ttacttctgc aactgctgtt ctattgccgg gcatggttct
      901 atctatttcc gtcataaatg atattttgca tacgaaccat ttcatcaata ccggcaattt
      961 cacttcagtc gggttgactg tttttgtttt cggtcaggca gttttgctgg ggaaccagtt
     1021 cactcgttcc ttttctcaga cgcgaactct tgggagaaag ctcagagcgc agaaaagaac
     1081 tttatcgcat ttcaatacaa ggctcgaaga agaagtaaaa acaagaacgt cggagcttga
     1141 gctggcgcgc cacaaagcgg agtccgccgc caatgcgaag actatgtttc tggcaaacat
     1201 gagtcacgaa cttcgcacac ctctcagcag catcattgga ttttcagaat tgcttcagag
     1261 agaaggcctc aatccggaag agtccgcaaa ctatatgcga attgtagcgg ggaatgctcg
     1321 tcacctgttg cagctcgtaa atgaagttct ggactacagc aaactcgaaa cgggcgccat
     1381 gcgatttgaa aaagtggatt tcagattgcg agacatgatt cttggtgtga ttgattctga
     1441 atccataaag gccgcatcgc gcggagacat tgtggaaatt gagattgatg ctgctgtgcc
     1501 ttccatcgta cgcggagatc ctactcgcat tgcacaggtt ctttacaatc tcgtgaacaa
     1561 tgcagtgaag ttcacggaca atggagtcat tcgagtagag atcaaggcgc tctcgcagga
     1621 tgcgcagact gtggatcttc aattctccat tacagacact ggaattggaa tcgcgcagga
     1681 acagcgatcg attatttttg atcgattcgg tcaggccgat tctggaatcg cgcgaaagta
     1741 cggcggaact ggactggggc ttgcgattgt gcagatgatc cttcaggcac tcggctcgtc
     1801 tttgcagctg gagagttctc ccggaaatgg aagtcgcttt tattttcatt tgaagttcga
     1861 gaaaggcgga gaagccgcca caatcctcga agctcctgtg tcaaacgaac ggctggcggg
     1921 cattcgggtt ctcgtggtcg atgacaacga aatcaatcgc atgcttgctg agaaactgct
     1981 gcagagatgg ggtgccactg tggacggcgc cgctgacgga gaagagggag ttcgaaaggc
     2041 gcagagcagt gcttacgatg tcatcttgat ggatctacaa atgccgggaa tggatggata
     2101 cgaggccgct cgaaggatcc gtgatttggg aatcaagacg cctttgattg cactttctgc
     2161 tgcagcactg gatgaggtga aggatcgtgc tctcgaagcg ggaatggacg attttgtatc
     2221 caagcctttt caccaggaag agttatactc tcgcctggtg cagcacactt cacccgatgt
     2281 ccgcaagaat cactgaccga tcgtcttcgc gttgttctgt gccagaaaac gcgagcacat
     2341 ccttgcgaat ggattccatt gcgtcttctg cgccgctggt caatgtgtgg cggaacatgg
     2401 acaaaaatcc cctttctccg agcatctggt tttcgcgtcc gtatgcttcc gtgattccgt
     2461 ctgtgtagac gatcaatctg gcagttccac ccagtgggat ttgtttttct ttgtagctta
     2521 ctttgcggaa caatccaaga acacgcccgg tctgtctgag aaaacgcact cgcattctat
     2581 ctccgtcttt ctggaacaga aatgctttag gatgtcccgc gttgcagtat ttgatgattt
     2641 tctttttcag attcacataa acgtagatgg ccgtgacaaa gttgtccgag agttttccat
     2701 acacagattc attcacgagt ttcattgtct tttctggtct gtccgcagac tcgaggcttt
     2761 cctcaaacgc gaggcgaagt gtattgaaga acatggcagc tccaatgccg tgacctgaaa
     2821 tatccgcaag aaagatagca tactcgtgtt ccgaaacgcg aatcacgttg tagtagtctc
     2881 caccaacact catgagtggg tcataccaga gagcgaactt gatgcgcgga tcatctgccg
     2941 gcagactctt ctggagattc ttgtcttgag acttggcggc gattttgatt tctgattcta
     3001 ccgttttctt ttccgcaaga atccgtttat tgagttcatc catttccgcg agagcctgtt
     3061 tcagtctcgc ggcagtttcc ctgtgcagaa tgcgaatgaa gttgtctgaa agttcctcta
     3121 ctacttcttt gctcaccagg tagtcaatgc ggctttgcgt ggagacacgg cgcagaagag
     3181 taaatgtgat ttctcttttt aattctattt ccgcagaata gatttcatca aacgaaactt
     3241 cgtagtcggt ggagaaaagc attctgcgaa ccagggtttc tgtatctgaa aacccgaagg
     3301 gtttttcttc gaggagcgaa tagaggcgat tcttataaat attgaagtaa ttcatcacct
     3361 tgtgagggct cagatggccg cgagcatgga gggccagaag ccatctacca atcatggagg
     3421 ttttgctgaa gtctttgccc actggataga aaacgcgagc ctgtagctct ttgagctgaa
     3481 aaataatttc gcgatgcgaa ggaacaagac cgggcatcat cttttcgaac gttgtgcgat
     3541 gctccgaaag tagcttctgc aagaagtaga tcatgtcgtc tcgcagaaac tcgtgcaggt
     3601 tttccttgat tctcttcttg tctacggacg gctgtccggt tcctttttcg tttatgagct
     3661 ggagtttggc caggaaaaga tctacttcga atttcagatc tcgcacccga tcctctgaaa
     3721 ccgactcgat cattcggtcc acttgctcct gcagtctttg tttttcgggg acgtccactc
     3781 tatgaccaga aaacagtaaa tggtttgatt ggaaagtaat acctgggccc atgacactat
     3841 gattcaggtc acagaggcag ccatcctggg catcattcaa gggctcacgg aattcattcc
     3901 tatttccagt accgcacatt tacgcatcat cccggccatg ctcgggatgg gggacccggg
     3961 ggccgcttac accgctgtga ttcagctggg gactcttttc tcactcatca tttatttctg
     4021 gcgcgacctt ctgaatttcg cgcaatcgtc gctgcagggt cttgcttccg gatctccatt
     4081 tagagacgaa aatgctcgca tggtttggta tctgggcgtt ggaactattc cagtatgcgt
     4141 gttcggtttg ttattcgcgc ataaaataaa gggcgacttt cgctcactgt acgtcatcgc
     4201 ggggagtctg atcgtttttg cgatcactct gtatatcgca gatcgcgttt cgcgtcgtca
     4261 taaaaccatt catcaatttg atctgcgcga tgcattgttg attggactgg cgcagagcgt
     4321 tgcattgatt ccaggcgctt ctcgttcagg catgactctg actatggctc tcttgctcgg
     4381 gtatacacgt gaatccggca tgcggatttc tttcctgctc agcattcctg ccattgcgat
     4441 ttccggtttg tatgagctgt atgaagaacg ccgtgcactc gcggaggccg gttttgtggg
     4501 tctggctgtg gccaccatcg tgtcggggat cgttggatac atgagcgttg ccttcttgct
     4561 gcgctacctg aagacgcatt ctaccactgc atttgttctg tatcgcattg ccatgggagt
     4621 cgtgatctta gggcttttgc gtgccgggta tttgatgtcc tgagggcttt tctcaaaaaa
     4681 ggccccttgc tcacacaatt cacataattc gcacaaattc ttcccatatc caacgcctac
     4741 cttgattgag gagttgcgat atgagtgaaa acaggattca gaagtatttt atccggccgg
     4801 tgcgaattgt tggaatggag ctgccggcct ggggttggat ggtcggatca tttgccgcca
     4861 tctgcttcct cgctgtctgt gtgtatgctt tgctgtacgc caacgtgttg cggtggctaa
     4921 attcttccat cttactctgg ggttcaggag tctttctcgg aatcgttttt ctgggattgt
     4981 ttctagttct acttcgcgcc gtgttttctt cgattcggca attcacgatc ttcaccctgg
     5041 tcctggcagc tgcatccttt gctactaacg cgtacgtcct gtcacatgaa gtattccctc
     5101 catttacatc cagaacggag gtagccctct gggtttgttt catcgcactt gcgatttcag
     5161 cactgaacgg gtggttaagc gatccgattc gatatgcatt gttacctctt ctgagtgcag
     5221 gcgcttttct ctgcgtcgcg tttgtcattg tgcttggacc gctgatggct tttgcgtggt
     5281 tctttgtctg ggcggcaggc ctcgggtttc ttccttatag tccacttttc gcgctcattg
     5341 ctttcgtttt gaatctgatg cggcttgagt cttctgtgcg gcagagggta gtcgcgcgct
     5401 ggctgatcgc cgggccggcc gccttgattg taatctactc catatactat aagatccagt
     5461 ggaatcgcgc agaggaggtg atgcgaagac cggagatctc tgcgggccac aatcgtctcg
     5521 atgaggatct tccactctgg attcagaagg ccgcgcgaat tcctgccaac catgtaacac
     5581 agatggttct acaaccaaat cgaagatctg aaatttccat ttttggtctg cagactctgt
     5641 ttgatcctct cgcatttctc agttctacct ctccctggag tgggtctcgc ggcgtggata
     5701 ttatttctgc agaagattca ggcagaatgc tgcacctgtt gtatggacaa agtcacgcct
     5761 atctggacag actgtggaga gggcattcgc tcatcactac gaatgttcag actcacgtgc
     5821 agattcaccc agagcttcgc gttgcttata cggaaactac tctttctatt ttcaacgaag
     5881 atcaagattc gaatcacaaa gtcatgggtt tgcggctcag tgcgggcaat gttcctcaag
     5941 aggccattta cacaatttct gttccagaag gaagcatttg cacaaaacta tctctgtggg
     6001 tgaacgggga agaacgcccg gcgagactta ccttcaaatc taccgcaaga cgcgcgtatg
     6061 aaacgatagt gagcgtggaa aggcgtgatc cgtcttacgt ggaatggctg gacgggaatc
     6121 gtcttcgcct gcgagtgttt ccggtctctc caggacaata tagaaccgtg cgagttggaa
     6181 tcgtctctcc tcttcgcgcc gatgaaggtc gcctgacgta caatagaatt gagtttgatg
     6241 gtcccgttca aacatttgcg gaacagtcag tgctcgtgga tttattttcc tccgctcgcc
     6301 tggatgttga gtcatccgga atgtttctga aagagaaagt ggtctcagac ggacaggtca
     6361 gacaatttcg gggatcgctt gttcgtccgc actggtcctt ttcttttccg gcacctgcag
     6421 caggtggttc catttccgca ggcggtaaga gctttctggt gcacccgctc cagatggaga
     6481 ataaaccgtt ttctccgcag gccattttag tcgcggtgaa cggttctcgt tcgcgagccg
     6541 cctggaagga attcataagt aaaacatacg gattgggtct gcctgtttat attctgacga
     6601 atgaatggtt tgagactgcg gacgtcaaaa aggcaatggc atacctggat gattgcgaaa
     6661 ttcccgcatt caatctgtat cctccagcct ttcccgccac gcgaaagctc gggcccgttt
     6721 tgtgggtcac ctccggagag gagcaatcca ttcctctagg tgaattacga gggtccattc
     6781 gattcgcgaa cacgcaaata gctgcaacag agaggccggc ggatgccgtg gcggttttga
     6841 acggtcgcgt ttct
//
LOCUS       DAOQJL010000160         6796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6226_length_6796_cov_4.778668, whole genome shotgun sequence.
ACCESSION   DAOQJL010000160 DAOQJL010000000
VERSION     DAOQJL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6226_length_6796_cov_4.778668"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..557
                     /locus_tag="PLG78_08685"
     CDS             141..557
                     /locus_tag="PLG78_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23230.1"
                     /translation="MYHRDYFIRMIQQFMVLLARLAGLKAKTDPEVILMETENGLRHF
                     TGLPPELIKSLTAASLASILNARQEGDYTTLAMTALLLKAEGEARVSIKDPEGVACLQ
                     KSIDLLGYLEVDRLPIELRSSMNFKEEVEQLLQKPW"
     gene            complement(613..1854)
                     /locus_tag="PLG78_08690"
     CDS             complement(613..1854)
                     /locus_tag="PLG78_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE23231.1"
                     /translation="MQSSAPSDTFSPYQKFIIVTIAVLQFTVILDFMVLSPLSALLLK
                     ELQVTTTQFGLVVSAYAFSAFASGIAAAGFADKFDRKRLLLFFYIGFLIGTLMCGLAP
                     DYYSLLVARIVTGLFGGVLGSIGGAIVTDLFPMSARGRVMGVVQMAFAVSQVFGIPVG
                     LLLANQFGWHAPFLMIVGFSSVVGVVIFTFMRPVRDHLHLNLGRHPMLHLWKTVTQWP
                     YIKAFLATTLMTTGGFMLMPFGAAFSVHNLGITNDQLPILYAITGVFSMIFGPIIGRL
                     SDRVGKYPVFAAGSLLTMIMVAIYTQRGITPFWFVIVLNVLLFAGISSRMISSMALMS
                     AVPEPHDRGAFMSINSSVQQLSGGVASAIAGTIVMQTRSGLIEHYDVLGYVVIVAIAI
                     TIAMMYWINLQIADKQAAHAR"
     gene            complement(1936..3072)
                     /locus_tag="PLG78_08695"
     CDS             complement(1936..3072)
                     /locus_tag="PLG78_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23232.1"
                     /translation="MRRFDPKYFLLFALATCSPQAQPGSSGDAPATSHYNDVARYLAG
                     LPIENPSAEVRKAIETPLYKNHVALMNQSWSAIEQTSLIPMREWKKQNLTDACEERLA
                     FYPLSGADFGNFYTMLPDCKNYLMIGLEDAGSVPEPFQLNDAQLASGLGQLERMVGQI
                     SIRNYFWTESMKTETANAYLEGVTPAILVFASRLGLNIRNIESVQVNDEGKVVVTRAV
                     PKDQRLKTLSGIRITFRAPGDKEDRTLQFLRIRIGPAAGSDTAPEGKFFKQYGDFITM
                     FKSAEYLMHFKLGEEFRSFVLQRSRLVIQDDSGIPYDKFTPEWTIKCFGDYVHPYPLV
                     QYTPQVQPGLAAAFAKERYPLPFPFGYGFYQGKGKSNLVIARKK"
     gene            complement(3172..4254)
                     /locus_tag="PLG78_08700"
     CDS             complement(3172..4254)
                     /locus_tag="PLG78_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23233.1"
                     /translation="MTRIAVISTLFLTLFAIPVFAEDHRFLVRGDAALGSISNAEAAE
                     DDYFYPLNNAIAGSLTIPSTTPSYKCPASLKPRMFETPEGSQERKDGCRSMASGVRAE
                     YRFQNMVRVFYDRFSLPNASYSTNFNTPVFAPGVNGVSQLQFGYNKREGRTRGGVGFY
                     YPVLEKLHIGVHIAQEKNFMHFGVTPASYTLYRVGNSSLSGLVAGAGIRELQTRGATP
                     GAGIEFDPMEYFGISLEYDRIRQRGSYSDNRVSGAASSTAGILSIGNPVIETIQAGLT
                     HSGYEAGLDLRFGKGRPIAFYLGGSQRKYVRSYQSYFYTTNSTFLAGPTITVLNNLYL
                     AGLYTSHRFIQNENLFHFKMELGLGF"
     gene            complement(4576..5334)
                     /locus_tag="PLG78_08705"
     CDS             complement(4576..5334)
                     /locus_tag="PLG78_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23234.1"
                     /translation="MVRYGQPRSAELVLITVAEPMDAKQRVKSDKPGPDIVYALKQNQ
                     ALNFQTGVYPYHYLNSLFWQIDNGRLLKAAMTSQEWCGTTTKVLLPQGGLDRLMYHSY
                     WDNEADGEMVLVRPDVQHPDRAILYDELPLIVRTPEAEHYRKLQVFPLLMSSQVTKPD
                     WDIYGEKRSPAYEPGTLEPESVDFMAMGVLRKARKITVRSGPGLKLVDEYFVEQNPAR
                     TLLGWNRHDGAHFVLRKISYAEYWKLNKPGDTLP"
     gene            5399..6430
                     /locus_tag="PLG78_08710"
     CDS             5399..6430
                     /locus_tag="PLG78_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE23235.1"
                     /translation="MKRGGLAGGPAQADMKNIPAQTEKILACSTLRIAYGTLSFFQGK
                     KKMNLRRDHIQPIQIAVQLASTPQAAHRAITSQTELRKWWAPRVIMSRNIVSQLNGKD
                     MEMKILQEDKYRLIRFTWRGADWKRDVPETVITYEITDLGAARGSTGQGIQIEMNHDG
                     WSDEEERNLQENIWQQALPGLQALLEGKKFTPWWEDEKNRSGDTGYKTADLKAFLERS
                     DKEGRGKNDRKKSIQVIWKICQAMDGVGEWIVKESTGEFELRARGALLFASLKGGILS
                     LNWRELEKLLGRELVDFANRLSLEQDLDLQIGKTQEKIIGKDLNPDLFSQWLQDVLDH
                     QKSDSKKKK"
     gene            6449..>6796
                     /locus_tag="PLG78_08715"
     CDS             6449..>6796
                     /locus_tag="PLG78_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23236.1"
                     /translation="MLPHVYSQATGMISFSWTALSEHIFEIRIKTPVTRVGTLYRLTA
                     AIYVLGLDIVSGTVDTAHEHGEEYADDTFLLRPSSDGETSFAYYSARVGVLMETLIRN
                     EKDPDDLLRERNIN"
BASE COUNT         1858 a         1675 c         1699 g         1564 t
ORIGIN      
        1 gatcgtgatt gttctttccg cactcttcct tggagaagca atcacctgga aggtggctct
       61 cggggccgct ttgattattg cagggaccat tgtggtgatc atctaacgtt agtcggccat
      121 gccgctggag aatccggagt atgtatcacc gcgactactt catccgcatg atccagcaat
      181 tcatggtgct cctggcgcgt ctggctggac tcaaagcaaa aacagatccg gaagtgattc
      241 tcatggaaac ggaaaatggc ctgagacatt tcacggggct gccgcctgag ctcatcaaga
      301 gcctgactgc tgcttcactc gcatcgattc taaatgccag gcaggaaggg gattacacca
      361 cacttgccat gactgctctt cttctgaaag cagaggggga agcgcgtgta tcgatcaaag
      421 atccggaagg agttgcgtgt ctgcaaaaat caatcgatct cctggggtat ctcgaggtag
      481 atcggcttcc gattgaattg cgttcttcca tgaactttaa agaagaagtg gagcagctat
      541 tgcaaaagcc ctggtgaaca actgcggcgg cagtgagcgc ctccctggaa ggcgcgtcag
      601 accgctgttc gcctaacgcg cgtgcgccgc ttgtttatct gcaatttgta gattgatcca
      661 gtacatcatc gcaatcgtaa tcgcaatggc aactatgacc acataaccca gcacatcata
      721 gtgctcaatg agtccgcttc tggtttgcat cacaatagtt ccggcaatcg cagaggccac
      781 ccctccggaa agctgctgca cggatgaatt gatgctcatg aacgcacctc tatcatgtgg
      841 ttcaggaaca gcggacatga gagccataga cgaaatcatg cgcgaagaaa tccctgcgaa
      901 aagaagaaca ttcagcacga tgacaaacca gaacggagtg atacctcttt gagtatagat
      961 ggctaccatg atcatggtca aaagcgatcc tgccgcaaat acgggatatt ttcccacgcg
     1021 atcgctgagt cttccgatga ttggaccgaa gatcatggaa aacactccgg tgatcgcgta
     1081 cagaatgggg agctggtcat ttgtaattcc caggttgtga acagagaatg cagcaccaaa
     1141 tggcatgagc atgaagccac cggtcgtcat caatgtggta gcgaggaatg ctttgatgta
     1201 tggccactga gttacagtct tccagaggtg aagcatggga tgcctgccca ggttcagatg
     1261 caggtgatcc ctcacgggtc gcatgaatgt gaaaattact acacccacaa cagaactaaa
     1321 accaacgatc atcagaaaag gagcgtgcca tccaaactga ttggcgagaa gtaatccgac
     1381 tggaataccg aacacctgac tgacggcgaa agccatctga actacgccca tcactcttcc
     1441 gcgagcagac atgggaaaca aatccgttac gatggctcca ccgatagaac caagaactcc
     1501 accgaatagt cccgtaacga ttcgtgcgac caggagtgaa tagtaatccg gtgccagtcc
     1561 acacatcagc gtgccgatca aaaacccaat atagaagaac agtaacaatc tcttacgatc
     1621 aaatttgtct gcgaatcccg ccgcggcgat cccagacgcg aacgcgctga acgcgtaggc
     1681 ggaaacgacg agcccgaatt gagtggttgt gacctgtaac tctttcagca ggagcgcgct
     1741 gagaggtgag agaaccatga aatcaagaat tactgtgaac tgtagaactg cgatggtgac
     1801 gatgatgaat ttctggtaag gcgaaaatgt gtcggaagga gccgaacttt gcatgagatc
     1861 tcctgggatt tgttacaaga acataaagaa gggagagaac gccatgcatt tgcccaggcg
     1921 atggcgcgtt tttttctact tcttgcgagc gatcactaga ttggacttac cttttccctg
     1981 gtaaaacccg tatccgaacg gaaagggaag aggatagcgt tccttcgcga atgcagcggc
     2041 cagacccggc tgcacctgag gagtgtactg caccagcgga tatggatgaa catagtcgcc
     2101 aaagcatttg atggtccact ctggagtgaa tttgtcataa ggaatgccag aatcatcttg
     2161 aatcaccaga cgactgcgct gcagtacaaa gctacgaaac tcttcgccga gtttaaagtg
     2221 catcaagtat tctgcggatt tgaacatggt gataaagtcg ccgtactgtt tgaagaattt
     2281 tccttcgggc gctgtatcgc tacctgccgc aggtccaatc cgaatccgca ggaattgaag
     2341 agtacgatct tctttgtctc caggtgcgcg gaacgttatg cgaattccgc tcaaagtttt
     2401 gagtctctga tcctttggca cagctcgggt tacaacgact tttccttcgt catttacctg
     2461 aacggattca atgttgcgaa tgttcaaacc aagtctggat gcaaaaacga gaatcgcagg
     2521 agtcactcct tcgaggtagg cgttcgctgt ttcagttttc atggactctg tccagaaata
     2581 attgcgaatg gaaatttgtc ccaccattcg ttcgagctga cccaggccgc tggcaagctg
     2641 cgcatcattc aactgaaatg gctctggaac agagcccgcg tcttccagac caatcattaa
     2701 atagttctta caatccggca gcatggtata gaaattccca aaatcggcac cggagagagg
     2761 atagaatgcc agcctttctt cgcacgcgtc cgtcaggttc tgtttcttcc attcgcgcat
     2821 gggaatcaga ctggtttgct caatggcgct ccaggattga ttcatcagag ccacgtggtt
     2881 cttatataga ggcgtctcga ttgctttgcg aacttccgca gaaggattct ctataggaag
     2941 acccgccagg tatctggcta catcgttgta gtgcgacgtg gctggagcat ctccggatga
     3001 accgggctgc gcctgcggag aacaggttgc cagcgcgaat aataaaaagt atttaggatc
     3061 gaatcttctc atggccttat gccaggaaac agccgaaagt gctgtaggca agcgaaaaca
     3121 agaccaggga agcgagtgct tcgcggaggc catccggact ccgcgaacga atcaaaagcc
     3181 cagacccagc tccatcttga aatgaaacag gttttcattt tgaataaaac gatgagaggt
     3241 atacaggcct gcgagataca ggttgttcaa aaccgtaatg gttggaccgg caagaaacgt
     3301 actgttcgtt gtataaaaat aagactgata gctccttacg tactttcgct gcgaacctcc
     3361 cagatagaac gcaattggcc gacccttgcc gaatcgcaga tccagacctg cttcataacc
     3421 ggagtgcgtg agaccggcct ggattgtttc aatcacaggg ttgcctatgg agagaatgcc
     3481 agctgtagaa ctcgcggcgc cgctgacgcg attgtcgcta tagctaccgc gttggcggat
     3541 gcgatcatac tcaagagata taccaaagta ttccattgga tcaaactcaa tgcccgcacc
     3601 cggcgttgct ccacgcgtct gcagttcgcg aatccccgct ccagccacaa gtccgctgag
     3661 agaagaattc cctactctgt acagagtgta ggatgcaggg gttaccccaa agtgcataaa
     3721 attcttttcc tgagctatgt gcacgccaat gtgaagtttc tcaaggaccg ggtagtagaa
     3781 accaacacct cctcgcgtac gtccttccct tttgttatag ccaaattgca gctgcgaaac
     3841 gccattcaca cccggagcaa aaaccggagt attgaagttg gtgctgtagc ttgcgttggg
     3901 taagctgaag cgatcataaa agacgcgaac catgttctgg aaacggtact cggctcgcac
     3961 tccactggcc atacttctgc acccgtcctt acgctcctga gaaccctcgg gtgtttcaaa
     4021 catcctgggt ttcaaacttg cgggacattt gtaggagggc gtcgtagaag gtatggtcaa
     4081 actacctgcg attgcgttgt tgagcggata gaagtaatca tcttcggccg cttctgcatt
     4141 ggaaatagat ccaagcgccg catctccacg gaccaggaat cggtgatctt ccgcaaatac
     4201 cggaatagcg aataacgtta agaacaaagt agatatgaca gcaattctag tcacaaaaat
     4261 cctccaaaaa tagcaaaacc aactcagtag gttgaagtgc aatttgagtt cttgtgtttt
     4321 actatctgtg cacgacagag aaagcgtaca tgagcagact accagagcca ttgcagtctt
     4381 gaaaattgaa atcgatcgtg aactcatttc acaaccgcaa tctcagcgtg cgcatttgcg
     4441 atcaagcaat acgcaaagtt ttataaacta ttttcagttt ttgtaaactg atatgctgtt
     4501 cattaccaga ttcaagacgt tcattcaatg attcggaatt tgtgaacgtt cagtctcagc
     4561 gccaggtgaa aaacatcagg gcagagtatc gcctggttta ttcagtttcc agtactcagc
     4621 ataggagatc ttacgtagta caaagtgagc tccatcgtga cgattccatc caaggagagt
     4681 ccttgcagga ttctgctcca caaaatactc atctacaagt ttaagtccag ggccgctgcg
     4741 aacggtgatc ttgcgagcct tgcgcaaaac tcccatcgcc ataaaatcaa cagactcggg
     4801 ctcaagtgtt cccggttcat atgccggact tcttttttct ccgtagatat cccagtctgg
     4861 ctttgtgact tgcgacgaca tcaaaagagg aaatacctgc agcttgcgat agtgttctgc
     4921 ttccggcgtc cgcacaatca gaggcaactc atcgtacaga atagcgcgat cggggtgttg
     4981 cacgtccggt cgaacgagaa ccatttctcc atctgcttca ttgtcccagt aagaatgata
     5041 catgagtcga tccagcccgc cctgaggaag gagaaccttg gtggtagtgc cgcaccattc
     5101 ctgcgaagtc atggcagctt tcagaaggcg cccgttgtcg atctgccaga agagggaatt
     5161 cagatagtga tatggataaa cgccggtctg aaaattgagc gcctgattct gcttgagcgc
     5221 gtacacaatg tccgggcccg gtttgtctga tttgaccctc tgttttgcgt ccataggctc
     5281 tgccaccgtg atcaaaacca gttctgcgct tctgggctgc ccgtatcgga ccacagaggc
     5341 atcataaatg gcgatctccg ccctgccctg aaaccctggt tcctttaaaa aagggaaagt
     5401 gaagcgaggc ggactggccg gcgggccggc acaggcggat atgaaaaaca tccccgcgca
     5461 aacagaaaaa atcctagcat gcagtacgct tcgcatagcg tatggaactc tctctttctt
     5521 tcaggggaag aagaaaatga acttgcgcag agatcatatc cagcccattc aaattgccgt
     5581 tcagctggcc tccacgccgc aggccgctca tcgcgcgatc acctcccaga cggaattgcg
     5641 aaaatggtgg gcgccccgcg tcatcatgtc acgcaacatt gtctcacaat tgaatggaaa
     5701 agacatggag atgaaaattc tccaggaaga taaatacaga ctcatccgat ttacgtggag
     5761 aggcgcagac tggaagcgcg atgttcccga aacagtcatc acctacgaaa tcacagatct
     5821 gggcgcggca cgcggaagca caggccaggg catccagatc gagatgaatc acgacggctg
     5881 gtcagacgaa gaggagcgaa atctccagga aaacatctgg cagcaggccc ttccaggact
     5941 gcaggcactc ctggaaggaa agaaattcac tccctggtgg gaggatgaga agaatcgttc
     6001 aggggatacc ggttacaaaa ccgcggatct caaagcattc ctcgaaagat cagacaaaga
     6061 aggacgcggc aagaacgatc gcaaaaaatc cattcaggta atctggaaga tctgtcaggc
     6121 aatggacgga gtcggcgaat ggatcgtcaa agaaagcaca ggcgaatttg aacttcgcgc
     6181 aagaggtgct cttctattcg catctttgaa aggtggaatc ctttctctga actggagaga
     6241 actggaaaaa ctcttgggca gagagcttgt tgattttgcg aatcgtctat ctcttgaaca
     6301 ggatctcgat ctacaaattg gcaagacgca agaaaagatc attggcaaag atctgaatcc
     6361 agacctgttc tcgcaatggc tacaggacgt gctggatcat caaaagagcg actctaaaaa
     6421 gaagaaatag ctttttggat tgacgggcgt gcttccgcac gtatattcac aggcaaccgg
     6481 aatgatctct ttttcctgga ctgccctgtc cgaacacatt tttgaaattc gaatcaaaac
     6541 accggtaacg cgagtgggta ccctctatcg cctgacagct gctatctacg tcctgggtct
     6601 ggacattgta tcgggaacag tggatacagc tcacgaacac ggagaagaat acgcagatga
     6661 tacttttcta ctacgccctt cttcagacgg agagacaagt ttcgcgtact attcggcacg
     6721 agtgggagtt ctcatggaga ctctcattcg aaatgaaaaa gacccggacg atttactgcg
     6781 cgaacgcaat atcaat
//
LOCUS       DAOQJL010000161         6793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6230_length_6793_cov_4.167112, whole genome shotgun sequence.
ACCESSION   DAOQJL010000161 DAOQJL010000000
VERSION     DAOQJL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6230_length_6793_cov_4.167112"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLG78_08720"
     CDS             <1..690
                     /locus_tag="PLG78_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23237.1"
                     /translation="RNDLDLHVICPSGEEIFYGHKQSVCGGWLDVDMNVQGETTKPVE
                     NVRWSRGTAPKGRYRIFVQNYRIHEGSGPTDYRVEIEINGEIKQFKGKMSAHGETSVE
                     SNQIIYEFDYDPSKRKVQDDPGEKATYAAYDDSVIRKQWASVLPSSHILPIADPARIL
                     DVMLGAMAITAGTADISSFARDLKGRDHTDAQVEEVRNALSGISIPSAGGSQFPGAGE
                     GENRQSGSRRI"
     gene            690..1985
                     /locus_tag="PLG78_08725"
     CDS             690..1985
                     /locus_tag="PLG78_08725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNE23238.1"
                     /translation="MARAVLLAGLQFGDEGKGSVTDFLCSRGYNLVVRYNGGPQAAHN
                     VVFWRNGQRVHHCFSQFGSGSFHGAKTILSRHMAIDPPALLEEGRVLESKGVPAFDLL
                     RVDEACTVITPYHRIANRMREMTRSVRHGSCGVGVGEAVLDREKGAPGIQVGENAGHL
                     RRKLEEIREYKIESLKGLRDFSQETESLFQDLLEKRVTEVFASYFQQIISRVRVSGEA
                     ELREAVKNHSTVFEGAQGVLLDRDSGFFPYVTPSKTTLALAEEWIAGLDLEVHRVGVM
                     RSYTVRHGAGPMPGETKEEALARVLRDEHNIDSPWQGSLRTGWLDLVLLRYALSVCKV
                     DSIAVTGLDRMAEWRFCEKYDLGGEDFYSLGPGGRGGEFTRRLMNASGYFSEPVDAGA
                     LLQKIESVCGVPVLIESTGPAAPDKKVHPDFPIPENNSG"
     gene            complement(1946..4261)
                     /locus_tag="PLG78_08730"
     CDS             complement(1946..4261)
                     /locus_tag="PLG78_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNE23239.1"
                     /translation="METVEGTLLDVYNFNDEIHLWIKTDSRALHFIDSFHPIIFADGP
                     VDLLKKLVTRFHELGAIHGQPVWTIRNHFYDNSPRRVLKITIAKPAILNRVRRRLYSF
                     YGKLDLYHTDIEIPVGYMFHKNIHPLSRVRIDYTTSGQTRNIERIVSLDRITDLEFDI
                     PPFKQIGIKLEKSHRLGLKDNRLIFETSRGTIALSAHNQKECLLQINRILAEEDPDII
                     VSSYGDQIIFPELFTLAQTHNIPLLFDRHGHHSLRKIVREGTSFNTYGSWIFRAASYP
                     LFGRLHVDAHNSFVFKESRLVGIFELARLARTPIQRMSRQSTGGALTSIETHVAIRKH
                     YLVPWQKSAVEKPKTAYDLLAVDKGGLIFVPNASETSVFENVAQLDYSQMYPSIMVNH
                     NISPECVNCPCCANDPSAPKVPEAGYHICKRRRGVVSDALAHILFRRKYYKTRLRELK
                     AQPETQHTISQIDFYDARQDSLKWMLVTSFGYLGYRNAKFGRIESHESVTAFGREKLL
                     LAKEIAEEEGYTLSHAITDCVFLHRQGQVLDKESILRMAESITNASGIEMSLDGIYSW
                     VIFLPSRQDRLLPVVNRYCGRFLDGSIKFRGIAARRKDTASFIRNAQLELLEIMAKGT
                     SIEHLRSLHSEVMELYDRKCSELRNGTVPWEQLLLRKTTSREFKDYQVKNATYLAMEQ
                     LSDLSLKVQAGEKVRYVVLNTKHPDRRKRYLSEESAALTRDSDVIPYDVAFYLKMLRE
                     AFYDLWSYFSPPDSFVEEDQPELFSGMGKSG"
     gene            complement(4248..4562)
                     /locus_tag="PLG78_08735"
     CDS             complement(4248..4562)
                     /locus_tag="PLG78_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23240.1"
                     /translation="MGRTHLPYSMQIDLVEDRLKKLRRGLRKEDQKIFDRLMRTARMQ
                     IQAGAMASSPNPFDTMALTMLIEIQRQIDVQSEQIKALQSVVLEIKNQSLLHTEKEDG
                     NS"
     gene            complement(4566..5216)
                     /locus_tag="PLG78_08740"
     CDS             complement(4566..5216)
                     /locus_tag="PLG78_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23241.1"
                     /translation="MLVRPTDSLRYFTAFSGKTGLLTGRSGIFHLSHLWQTHLSLLGQ
                     RVLVIDCAIRFNAFYLADEAMRMGLQTEALLQSILVQRAFTPYQILDVLYGILDAAHK
                     PPDSTYFLLAPFKQFFDGDVSDEEGRFLLKKLTSAVSLIQRESIPLLIVEKDSYSHPS
                     FAPAFEDLKSIASIQWRLTDTDGIFSVRIEQLPMRQASIIHKPVSIPSTNLQINSA"
     gene            complement(5287..>6793)
                     /locus_tag="PLG78_08745"
     CDS             complement(5287..>6793)
                     /locus_tag="PLG78_08745"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNE23242.1"
                     /translation="LMHEGKDVVMYPEGVPGIGKGFDKRYQIQKFSTSFLRMATEFNA
                     ELIPFHTVNAEYLHPYSYKSDRLNRFVQKLGVPFLPLSPLTALVPLFPWMFYFSLPAN
                     LHFVFADPIQPSDLTSKSHQELKRSDYIRMRDDIHSDFQKGINIHVEEFGQDPFRLGE
                     FMEQLLSNPERALFTLPTGWPILITGLQKLYDDGLKKELKYTMDEFISLAARATDGLS
                     FSLPMSWPVVFGLKGFSVDIMRQIGHFMEEELAMATPEPLPEKPTPAEPSAKEAATAE
                     NLNEEASYAEAIQLLDDFEANAKNLDRATNILLPLTRGKNPGRAFGLLCQAQYWRGME
                     ATANKISIYEKGIEFGEAGVERDPGSVESHFWLAVNYGLLGETRGILESLFLVKPIEA
                     HLHKAIELDESYFFGGPHRALGWVLHKLPPWPLSHGSNQEALEHMERAIELGANFPIN
                     LLYAAHICVSLKDKKKAREYCDQLLGMEINPRHSREETRYRQEALAMIAKI"
BASE COUNT         1671 a         1497 c         1803 g         1822 t
ORIGIN      
        1 agaaatgatc tggacttgca tgtgatctgt ccttctggag aagagatctt ctacggccac
       61 aagcaatccg tatgtggtgg ctggctggac gtggatatga atgttcaggg cgaaaccacg
      121 aaaccagtgg aaaatgtaag atggagtcgt ggcacggcac caaagggaag atacaggata
      181 ttcgttcaga actatcgaat tcacgagggc tccggtccaa ccgactatcg cgttgaaatt
      241 gaaatcaatg gagaaatcaa acaattcaaa ggaaagatgt ccgcgcacgg agaaactagc
      301 gtggagagca accagatcat ctatgagttc gactatgatc catccaaacg caaagtgcag
      361 gatgatcccg gagaaaaggc aacgtacgcg gcgtatgatg attcagtgat tcgaaagcaa
      421 tgggcgagcg tactgccgtc gtcgcatatc ttgcccatcg cagatccagc aagaattctg
      481 gacgtgatgc ttggcgcgat ggccatcacc gcgggcacgg cagatatttc ttctttcgca
      541 cgggatttga aggggagaga ccacacagat gcgcaggtag aggaagtgcg aaatgcattg
      601 tccggaatct ccataccatc cgccggcggc tctcagttcc ccggtgcagg agagggagaa
      661 aacagacaat ctggttcgcg tagaatttag tggcgcgcgc agtacttctg gcagggcttc
      721 aatttggcga tgaaggcaaa ggttccgtaa cagacttcct gtgcagtcgt ggctacaacc
      781 ttgtagtgcg atacaacggc ggtcctcagg cggcgcataa cgttgtattc tggcgaaatg
      841 gacagcgcgt tcatcattgc ttttctcagt ttggctccgg gtcgtttcat ggagcaaaga
      901 caattttgtc caggcacatg gccatagatc caccggctct tctagaggaa ggacgtgtac
      961 tggaatcgaa aggtgtcccc gctttcgact tgctgcgcgt agacgaagcc tgcaccgtca
     1021 ttactccgta tcatcgcatt gcaaatagaa tgcgagaaat gactcgatcc gttcgccatg
     1081 gaagctgcgg agttggagta ggggaagctg ttctggatag ggagaaagga gcgcctggaa
     1141 ttcaggtggg cgaaaatgca ggtcatttgc gccggaaact tgaggaaata cgcgaataca
     1201 aaatcgaatc tctgaaaggg ctccgtgact tcagccagga gacagagtct ttgttccagg
     1261 atttactcga gaagagagtt acggaagttt tcgcatctta tttccagcag atcatttcgc
     1321 gcgttcgtgt ttctggagaa gcagagcttc gggaagctgt gaagaatcat tccacggtat
     1381 ttgagggggc acagggagtt cttctcgatc gagattcagg tttctttccg tacgtaacgc
     1441 cctcgaaaac cacactggct ctggcggaag aatggatcgc cgggctggat ctggaggtcc
     1501 atcgagtcgg cgtcatgcga agttacacag tgagacatgg ggccggaccc atgcctggtg
     1561 aaacaaaaga ggaagctctg gcccgagttc tcagagacga gcacaatata gattcaccct
     1621 ggcagggaag tctgcgaact ggctggctgg acctggttct gctacgttat gcgctatccg
     1681 tatgcaaggt agattcgatc gccgtgactg gactggatcg aatggcggaa tggagattct
     1741 gcgaaaaata tgatctgggc ggggaagatt tttacagcct cgggcctggt gggcgcggag
     1801 gcgaatttac acggagactg atgaatgctt ctggctactt ttctgagcca gtggatgcgg
     1861 gtgctcttct tcagaagata gaaagtgttt gcggagttcc tgttttgata gaatcaacag
     1921 gtccggcggc accggacaag aaagttcatc ctgattttcc catcccagag aataattctg
     1981 gctgatcttc ttctacaaaa gagtccgggg gggagaaata ggaccagaga tcatagaaag
     2041 cctcccgtag cattttcaga tagaacgcaa catcgtaagg gatgacgtct gaatctcgcg
     2101 tgagtgccgc cgattcttct gagagatatc tttttcttct gtctggatgt ttagtattaa
     2161 gtactacgta tcgtactttt tcacctgcct gaaccttgag tgatagatcg gacaactgtt
     2221 ccatggccag ataggtggcg tttttgacct gatagtcttt aaattcacgg ctggttgtct
     2281 ttctcagaag aagctgctcc cagggaacgg ttccatttct gagttcgctg cattttcggt
     2341 cgtataattc catgacttct gaatgaaggc tgcgcagatg ttcaatggaa gtgccctttg
     2401 ccattatttc tagtaattcg agctgcgcgt ttctgataaa agaagccgta tcttttcttc
     2461 tcgctgcaat tccacggaat tttatgcttc cgtccagaaa tcggccacaa taccggttga
     2521 ccactggaag caatctgtcc tgacgagaag gaagaaaaat cacccaggaa tagatgccgt
     2581 caagagacat ctcaattccg gaagcattcg tgatggattc tgccatgcga agaatagatt
     2641 ctttgtccag aacctggccc tgtctgtgga ggaacacgca gtcagtgatt gcatgagaca
     2701 atgtatatcc ttcttcttca gcgatttctt tggcaagcag tagcttctcg cggccaaagg
     2761 ctgtgacgct ttcgtggctt tcaattctgc caaactttgc attgcgatag cccagatatc
     2821 caaaagaagt aacgagcatc catttgaggc tgtcctgtct tgcatcgtaa aaatcgattt
     2881 gagaaatcgt atgttgcgtc tccggttgcg ccttcagttc gcggagtctt gttttgtagt
     2941 atttgcgtct aaaaagaatg tgagcgagag cgtcagaaac cactccgcgt cttcttttac
     3001 atatatgata tcctgcttcc ggtacttttg gagcggaagg gtcattggca cagcacggac
     3061 agttcacgca ttcaggcgaa atgttgtgat ttaccatgat gcttggatac atctgactgt
     3121 aatcgagctg ggctacattt tcaaacacgc ttgtttcaga tgcgttgggc acaaagatca
     3181 atccgccttt gtctacagcc aatagatcgt atgctgtttt tggcttttct actgcacttt
     3241 tctgccacgg caccagatag tgttttcgga tggctacgtg agtttcaatg gatgtaagtg
     3301 cgccgcccgt ggattgccgt gacattctct gtatgggtgt gcgcgccagt cttgccagtt
     3361 cgaatattcc aaccaggcgc gactctttga atacgaagga attgtgtgcg tccacatgaa
     3421 ggcggccgaa tagaggatag gaggcagcgc gaaagatcca gctgccatac gtattgaagc
     3481 tggtcccttc gcgtacgatt ttacgcagac tgtgatgacc atgtcgatcg aatagtagag
     3541 ggatattgtg agtttgtgca agagtgaata gttcaggaaa tatgatttga tctccataag
     3601 aagaaacaat gatatccgga tcttcctcgg cgagtatgcg attgatctgg agcaggcatt
     3661 ctttctggtt gtgagcggag agagcaatgg tcccacggct tgtttcgaaa atgagacgat
     3721 tgtctttcag ccccagtctg tggctctttt ccagctttat tccgatttgt ttgaacggtg
     3781 gaatatcaaa ttccagatct gtgatgcgat ccagggatac aattcgctcg atgttcctgg
     3841 tctggcctga agtagtatag tcgattcgaa cgcgcgaaag tggatgtatg ttcttgtgaa
     3901 acatgtatcc aactggaatt tcaatgtctg tgtgatatag atccagtttg ccgtagaagg
     3961 agtaaaggcg tcttcgcacg cggttgagaa tggcgggttt ggcaattgtg atttttaaaa
     4021 ctcttctggg cgagttatcg tagaagtgat ttcggatggt ccagactggc tggccatgga
     4081 tggctcccag ctcatggaat cttgtgacta gcttttttaa aagatcaacc gggccgtctg
     4141 cgaatatgat tggatggaag gaatcgatga agtggagtgc cctggaatct gttttgatcc
     4201 agagatgaat ttcatcgttg aaattgtata catccagaag tgtgccttca actgtttcca
     4261 tcctctttct ccgtgtgcag aagggactgg tttttgattt caagtacgac gctttgcaat
     4321 gctttgattt gttctgattg aacgtcgatt tgtctttgga tttctataag catggtgagt
     4381 gccattgtat caaaaggatt cgggctggac gccatggctc ccgcctggat ctgcatgcga
     4441 gccgttctca tgagccggtc gaagatcttt tgatcttctt ttctgaggcc acgtcttagc
     4501 tttttgagtc gatcttctac aagatcgatc tgcatggaat agggaaggtg cgttcgtccc
     4561 atacgttatg cggaattgat ctggagattt gtggacggaa tggagactgg tttgtgaatg
     4621 atggatgcct gtctcatggg gagctgttca attcttactg aaaagattcc atctgtatcc
     4681 gtgagccgcc attgaatgga tgcaatggat tttaaatctt cgaaggcggg agcgaaagaa
     4741 ggatgagagt acgaatcttt ttctacgatc agaagaggaa tggactccct ttgtatgagg
     4801 gaaacggcgc tcgtgagttt ctttaaaagg aatcgtcctt cctcatcaga cacatctccg
     4861 tcgaagaact gcttaaacgg agccagcaag aagtaagtgc tgtccggtgg tttgtgtgct
     4921 gcgtccagga ttccatacag cacgtccagg atctggtatg gagtgaatgc tctctgtacg
     4981 agtatggatt gaagcagcgc ttctgtttgc agtcccatgc gcatggcttc atccgccaga
     5041 taaaacgcgt tgaatcgaat ggcgcagtca atgaccagga ctctctggcc aagaaggctg
     5101 aggtgggttt gccacagatg ggatagatga aagattccgc ttcttccagt caaaaggcct
     5161 gttttgcctg aaaaagcagt gaaatagcgc aatgaatccg tggggcgaac cagcatgatc
     5221 catactagtg tcggctatac aaatgtcaag tattagcctt tcttttcgca acggagctct
     5281 gtttgctcaa attttcgcaa tcatcgcaag cgcttcctgt ctgtacctgg tttcttccct
     5341 gctgtgacga gggttgattt ccatacccag caattgatcg cagtattctc tggccttttt
     5401 cttgtctttc agagatacgc agatgtgcgc ggcatacagt aggttgattg gaaaattcgc
     5461 gccaagctca atggcgcgtt ccatgtgctc gagtgcttct tgattgctac catgcgaaag
     5521 aggccatgga ggtagtttgt gtagaaccca tccaagagcg cgatgagggc cgccaaaaaa
     5581 ataggattcg tccagttcga tggctttgtg cagatgcgct tcgattggtt tgaccagaaa
     5641 cagactttcc agaatgcctc tcgtttcgcc taacagaccg tagttgactg ccagccagaa
     5701 atgagattct acgcttccag gatctctttc cacccccgcc tcaccaaact cgattccctt
     5761 ttcgtaaata gagatcttgt ttgccgtggc ttccattcct ctccagtatt gagcctgaca
     5821 tagaagtcca aatgcccgac ccggattttt tcccctggtc agaggtagaa gaatgtttgt
     5881 ggctctgtcc aggtttttgg cgttcgcttc aaagtcgtcc aggagttgaa tcgcttccgc
     5941 gtaggaagcc tcttcattta gattctcagc agtcgcagcc tctttcgcag atggttccgc
     6001 aggtgtgggc ttttccggca gaggctccgg tgttgccatg gccagttcct cttccatgaa
     6061 atgaccaatc tgccgcatga tatcgacaga aaatcctttc aggccgaata caacaggcca
     6121 ggacatgggg agagaaaagg acaggccgtc cgtggcacgg gctgccaggg aaataaattc
     6181 atccatcgta tatttgagtt cttttttcag gccatcgtca tagagttttt gcagacccgt
     6241 gatgagaatc ggccaaccgg ttggaagagt gaagagagct ctttcggggt tggatagaag
     6301 ctgttccatg aactcgccca ggcgaaacgg atcctgaccg aactcttcta cgtgtatgtt
     6361 gattcccttc tgaaaatcag aatgaatgtc gtctctcatg cgaatataat cgcttctttt
     6421 cagttcctgg tgcgatttcg aggttagatc ggatggctgg atcgggtctg caaaaacgaa
     6481 atgaagattc gcgggcaggg agaagtagaa catccaggga aaaagaggaa caagggcagt
     6541 taacggggac agaggcagaa acggaacccc cagcttctgc acgaaacgat tcaatcgatc
     6601 gcttttgtag gaatacggat gcaagtactc tgcattcaca gtgtgaaatg gaatcaactc
     6661 tgcgttgaat tcagtagcca tgcgaagaaa agacgtcgag aatttctgga tctggtatcg
     6721 cttatcaaat ccctttccga ttccagggac tccttctgga tacatcacta cgtctttgcc
     6781 ttcatgcatc agg
//
LOCUS       DAOQJL010000162         6753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6281_length_6753_cov_4.665273, whole genome shotgun sequence.
ACCESSION   DAOQJL010000162 DAOQJL010000000
VERSION     DAOQJL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_6753_cov_4.665273"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2780
                     /locus_tag="PLG78_08750"
     CDS             96..2780
                     /locus_tag="PLG78_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23243.1"
                     /translation="MEDSTPSASMGRITSRKLSQAIRINSKTGALVAAAISGLFCSNH
                     SRLPVLYMPPESPEIRLEFAGMDLIRQTRTREETGPLPGTRISEVAITDDQCIRSHSP
                     GFFEHEEGLRFEICGTGIDREQFETSMARRERASMGHLFELREISLTEIRKLNSEIVV
                     DHAFDPLSVQSTLTLNTGGNLPAVYPLHAPRVFADIQHLSIVFARRDSRFMILETSDP
                     RIVAPFLSGLSRVPGITNNLRGSADVYVSEVKQGKDAFLEISSRTAGRAKLRILRGAS
                     VENLELYFFGGSAYVFDHADAVELPSGVFSITADQYETAGGNATRSVTVRDEHLVPTA
                     CLQLCDSRGISSFPSGTSDDACKPEDLELTELSPAGVYTDHVDASGKFLEVRVKRSCM
                     LGSVLLRAGSTIDPGQGKVESGARLLFASSSEFFSEKVLDADLRSLNYSTPVEAFDSE
                     GHAAILYSPDADARWADSSGFVHSIVLDQGVAIYHDRSRVGLRPDLTSNAMSPGLPEI
                     SEAPHSSFSLSEVSLMGSWNGSSVPDDEFIELTGKEGGPGRWTVDVKRLSDGKTIRHH
                     FPPQDGIAAYFRKSPVCFPGYFGRADLFLPNEPVELSWFDSHGENQGHASVSPALATS
                     MSSAYASAVWHESTHSLRVHSPIVGICAKANASPGAPGSPSPFFAEEPSPAGRIFRFY
                     SDVAASISVEIDGLSSSRMAANEDLISVTALRPRSVFRITASGAQVSGEVLLSPGLAV
                     TGVHPSPVAPGVEWIRVCATAPFFPQTNLYVHDEDSEDRIVPFSQRTGRALLSSTMNL
                     PAFGCALIVDPDYTGQSFPPPPFPFYDYAGWSISSTSAIGSGLGADESVWITNATGDI
                     LATYGLPDIAPFHVHASSGQTIVRTWPSYDAPESWSAQ"
     gene            2777..4171
                     /locus_tag="PLG78_08755"
     CDS             2777..4171
                     /locus_tag="PLG78_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23244.1"
                     /translation="MKSFIRFFSSSFLMPAFLHVAASVFVSDTLYAIENDDPVYAGRS
                     PLSVSFLAQGIRSSVYPDRLKDALEFSSDELSAGVHVKGSSLDAEENSFLYSADAAAL
                     FSAQGKVQLLRGSLFAAGYSGCSASGCGIMLGQFYPDPRGSVGVDPIGGNAHSSHASF
                     LGDAEDGRSGTAVFFRGEHAGVEFYPAIVDLNNAEARGARAQAFLFFKNAGMSVHYEG
                     IQQGSQAGKGKDRLDYTGAGFYIDKPDGFIQFYTRIAVIHSQGRYTTMHTGETHSTRA
                     SLEGQALKAGAMFSVGWFHLYADGFLPEPPSNVDGNRTSDEKSGYTGWNQFSGTNIFL
                     REIDAVPFARLCRAKENCTGIESSTSFRAFRFAAGVARARVSLEMPRLELSLAGSLFR
                     PLAPRTDHSGNPYSKIRRDHKIPELREITVQVSIQHEVGRLLFEYGRLSAKSDEGSKL
                     AAERFTLSLERAAF"
     gene            4212..5816
                     /locus_tag="PLG78_08760"
     CDS             4212..5816
                     /locus_tag="PLG78_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE23245.1"
                     /translation="MGRLSTLFFLILIHESCIHRSRPEFPGVLLDQASHDFLFTRPDV
                     YDPSIRDRIVHEIDGAKESISLYCYEIDEPEILRALDRARGRKILLKIVGSPDQSYAE
                     LRESGFAFRIRARSGLQHSKVWIIDHRLLISGTGNFTQSDLFHNNNAFFFLNVSTNTA
                     MEVDRSLENADANASPVFLPFGGVLLVSPSRGKLIQTRLILAALNAKKTLRYMIFSHT
                     DQVLSSAMAESAMRGVLIEGIYDAESDGLPDEGVQLNIATRAFIYTDGNRSEFQTEDG
                     VVHGGHLHHKTMVVDDEMVLTGSYNFSIGARDSNQEAYFEFHDPAVTWLFNQEFERVV
                     SSARLEGKPPFPTSGLPVAFESPLWCGTIRRLTVFFGSGASAGAQHFRASDSCVSANQ
                     KASESAGTARSSWMVPGQTLAIHSLSWRGNFAGSTPGIPSVKKAVSIKRATTDRLWVD
                     SQTRYSALMAWVRDGLVNIQFQESAPGVLTFAPLPPGDAIVFLWSGGVWETACLQSGT
                     SLDSAPSAFLQALELEGIHVSCTAIE"
     gene            5898..>6753
                     /locus_tag="PLG78_08765"
     CDS             5898..>6753
                     /locus_tag="PLG78_08765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE23246.1"
                     /translation="MISIPGYRFTDTISEGDKIAVYRGQRLSDGQPARVRAIGGRFPD
                     PRDVARMRRDHEISSALEVPGVIRSLGLFPHQGGLAIVLEDPGAVELARATEQGVHSI
                     FDFLRIGIQVAEILAQVHKKRIVHKNLHPGSILVHPTTGHVYITDFGISSTLGRENAG
                     LLTPDQIEGQLPYIAPEQTGRMNRSIDYRSDFYSLGITLFEILTGRRPFESNDPMEFV
                     HFHIARDLPQAGKARYGDAVPEGIQQVLSRLTAKNPEDRYKSAAGLARDLTECLKQLQ
                     EKGTISNFT"
BASE COUNT         1612 a         1807 c         1704 g         1630 t
ORIGIN      
        1 tcaatgccgc caggttcggg ccgaaaaaaa tcagtaacga gcagaactcg cttcaacttt
       61 gatccagccc gaggaatccc aaaaagtggg aacgtttgga agattctaca cccagtgcta
      121 gcatgggacg catcacttcc aggaaacttt cacaggccat tcgcatcaac tccaaaacgg
      181 gcgcgcttgt ggccgcggcc atctcggggc tgttttgtag caatcattct cgccttcctg
      241 tgctctatat gccgccagaa agcccggaga tccggctgga atttgctgga atggatctca
      301 ttcgtcaaac tcggaccaga gaagaaacag gcccacttcc cgggacacga atttcggaag
      361 ttgcgattac ggacgatcaa tgcattcgct cccactctcc cggatttttt gaacatgaag
      421 agggtcttcg atttgaaatc tgcggcacag ggatagaccg ggaacaattt gaaacgagca
      481 tggcgcgacg cgaacgcgca tccatgggtc atcttttcga gctaagagaa atctctctca
      541 ccgaaatccg aaaactcaat tctgaaatcg tagtcgatca cgcgttcgat ccactttccg
      601 ttcagtccac acttactctg aacaccggcg gaaaccttcc ggcagtctat ccacttcatg
      661 cgcccagagt cttcgcggac atacaacatt taagcattgt gtttgccaga cgcgactcca
      721 ggttcatgat cctggaaaca tccgatcctc gcatcgtcgc gccgttcctg agcggtcttt
      781 cgcgtgttcc aggcatcacc aataatcttc gcggatctgc ggatgtatat gtcagcgaag
      841 tgaagcaagg gaaagatgcc ttcctagaaa tttcttcgcg aactgctgga cgcgcgaagc
      901 ttcgcatcct tcgaggagca agcgtagaga atctggaatt gtatttcttt ggaggaagcg
      961 cctacgtgtt tgaccacgca gatgcggtag aacttccgtc tggagtattc tccatcaccg
     1021 cggatcaata tgagacggcc gggggcaacg ccaccaggtc tgttactgtg agagacgaac
     1081 atctggttcc caccgcctgc ctgcagttat gcgattcgag aggaatctcc tccttcccgt
     1141 ccgggacgtc ggatgacgca tgcaagccgg aggatctgga actcaccgaa ctttcgccag
     1201 caggagtgta cacagaccat gtggacgcgt ccggaaagtt cctggaagtg cgcgtgaaaa
     1261 gatcgtgcat gctcggttcg gttctactcc gcgcgggttc caccatagat ccgggtcagg
     1321 gaaaagtcga atccggcgca aggcttctct tcgcttcttc cagcgagttt ttctcagaaa
     1381 aggttttgga cgcagatctt cgctcattga actatagcac gccagtagaa gccttcgatt
     1441 cagagggcca tgcagccatt ctgtattcac cagatgcaga tgccagatgg gcagactctt
     1501 ccggcttcgt tcactccatt gtcctggatc agggcgttgc catctatcat gacagaagca
     1561 gagtcggact tcgtccagat ctaacgtcca acgctatgtc tcccgggctt cctgagatat
     1621 cagaagcgcc acattccagc ttttctctat ctgaagtgtc tctgatgggg agctggaatg
     1681 gaagctctgt tccagacgat gaattcattg aactaacagg aaaggaaggt ggtccaggac
     1741 gctggacggt agacgttaag cgactttctg atggaaaaac aatccggcat cattttccgc
     1801 cgcaggatgg aatcgccgca tattttcgca aaagccctgt ctgttttcca gggtactttg
     1861 gccgcgccga tctctttctt ccaaacgaac cggtagaact ctcctggttt gattctcatg
     1921 gagaaaatca aggccatgca agcgtatccc ctgcactcgc cacttccatg agtagcgcat
     1981 acgcaagtgc cgtctggcat gagtctacgc actctcttcg cgtacattcg cccattgtcg
     2041 gaatctgcgc aaaagcgaac gcaagtccag gcgctccggg ttctccgtct cctttcttcg
     2101 cagaagagcc atcacctgcc ggacgaatct ttcgcttcta ttcagatgtt gcggcttcta
     2161 tctctgtaga aatagacggc ttaagttctt ctagaatggc cgcaaatgaa gatctcatct
     2221 cagtgacggc gcttcgcccc aggtccgttt ttagaattac agcgtcaggc gctcaggttt
     2281 caggtgaagt cctgctgtca ccgggacttg cggtcacagg agtgcatcct tctcccgtgg
     2341 ctccgggcgt agaatggatt cgagtctgtg cgactgcacc gttttttccg caaacaaatc
     2401 tctatgttca tgacgaagat tcagaagata gaatcgttcc tttttcgcag cgcacaggac
     2461 gcgctcttct ttcttctact atgaatcttc ctgcgttcgg atgcgctctc atcgtagatc
     2521 ctgactacac cggccaatcg tttccaccac ctccctttcc gttttacgac tatgctggct
     2581 ggtccatctc ctccacttct gcgattggtt ctggactggg cgcagacgaa tcagtctgga
     2641 ttacgaatgc aacaggcgac attctggcca cgtatggcct gccagacatt gctccttttc
     2701 atgtgcatgc atcctccgga caaaccatcg ttcgcacatg gccttcctat gatgcgccag
     2761 aatcctggag cgcacaatga aatctttcat tcgattcttc tccagttcgt tcttgatgcc
     2821 cgcattcttg catgtggcgg catccgtttt cgtttcggac acgttatacg ccattgagaa
     2881 cgacgaccct gtctatgcag ggagatcacc gctttccgtg tcgttccttg ctcagggaat
     2941 cagatccagc gtctatccgg acagactcaa agacgctcta gaattttcct cagatgagct
     3001 gagtgctggt gttcacgtca aaggctcttc tctggatgca gaagaaaact cgtttctata
     3061 ttccgcagac gcagccgcac tcttcagcgc tcaaggcaaa gtccagctac ttcgaggctc
     3121 tctatttgcc gccggatatt ccgggtgcag cgcttctgga tgcggaatca tgctcggaca
     3181 attctatccg gacccacgag gatcggtggg cgtggacccc attggaggca atgcacattc
     3241 ttcgcacgct tctttcctgg gagacgcaga agacgggagg agcggcactg cggtcttctt
     3301 tcgcggagaa catgcgggcg tagaattcta tcctgcgatc gtagatctga acaatgcgga
     3361 ggcccggggc gcaagggccc aggcatttct attttttaaa aacgctggaa tgtccgttca
     3421 ttacgaagga atccaacagg gcagccaggc gggtaaagga aaagaccgtc tggattacac
     3481 gggcgccgga ttctacatag acaagccgga cggattcatc cagttctaca cacgcattgc
     3541 ggtgattcat tctcagggac gttatacgac catgcacaca ggggaaacgc attccacgcg
     3601 ggcctctcta gaaggtcagg cattgaaggc cggagccatg ttttctgtcg gatggtttca
     3661 cctctacgca gatggttttc tgccagagcc tccgtccaac gtggacggga atcgcacgtc
     3721 cgatgaaaag tcaggataca ccgggtggaa tcagttctca ggaacaaaca tcttccttcg
     3781 cgaaatcgac gcagttcctt tcgcgaggct ttgtcgtgcg aaggaaaact gcaccggaat
     3841 cgaatcatca acttcctttc gcgcttttcg cttcgcggct ggagtggctc gcgcgagggt
     3901 ctccctggaa atgcctcggc tcgaactttc tctggcagga tcactgtttc gtccgctggc
     3961 gcctcgaaca gaccactctg gcaatcctta ctctaaaatt cgtcgcgacc ataaaatccc
     4021 ggagctcagg gagatcactg tccaggtctc catccagcat gaagtaggga gattgctatt
     4081 cgaatatgga agactgtccg cgaaatcgga cgaaggctca aagctcgccg cagaacgatt
     4141 caccctttcg cttgagcgag ccgctttttg attcgggcag aacgatttgc acatcggata
     4201 ccagtacagg aatgggaaga ctttcaacac tgttcttttt gattttgatt catgaatcct
     4261 gcatccatcg atcaaggccg gagtttccag gggttctttt ggatcaggca tcgcatgatt
     4321 tcttgttcac gagaccggac gtatacgatc cttctattcg agatcgaatc gtccatgaga
     4381 tagatggtgc aaaagagtcg atcagcctgt attgctatga aatagatgaa ccagagattc
     4441 ttcgcgcgct cgaccgcgcc agagggcgca agattcttct aaagattgta ggatccccgg
     4501 atcaaagtta tgcggaattg cgcgaatccg gttttgcctt tcggatcaga gccaggtcag
     4561 gtttgcagca ttccaaagtg tggattatag atcacagact gctgatttct ggcacaggaa
     4621 attttacgca gagcgatcta tttcacaaca acaatgcatt cttttttctg aatgtgagca
     4681 caaatactgc catggaagtg gacaggtccc tcgaaaatgc agacgcaaat gcatctccag
     4741 tattccttcc attcggagga gttcttctgg tttcgccctc tcgcggaaag ctcatccaga
     4801 ccagactcat tctggccgcg ctcaatgcta aaaaaacatt acgatatatg atcttcagcc
     4861 acacagacca ggttttatcc tctgccatgg cagaaagcgc tatgcgagga gttcttatag
     4921 agggaatcta tgacgcagaa agcgacggac tgccggacga aggcgttcag ctaaacattg
     4981 ccacaagagc ttttatatac acggatggaa accgttctga attccaaact gaagacggtg
     5041 tagtgcacgg tggacatctt catcataaga ctatggttgt agatgatgag atggttctaa
     5101 cggggtcgta caatttttcg attggagcgc gcgattccaa tcaggaagcc tattttgaat
     5161 ttcacgatcc tgctgtaacg tggcttttca atcaggaatt tgagcgagtg gtatcgagtg
     5221 caagactgga aggaaagcca cccttcccca ccagcgggtt gccggttgca ttcgaaagtc
     5281 ctctatggtg tggaaccatt cgaaggctga cagtattttt tggaagcgga gcttccgccg
     5341 gtgcacagca ctttcgcgcc tccgattcat gcgtaagtgc aaaccagaag gcgagcgaga
     5401 gtgcggggac agcaagaagc tcctggatgg ttccggggca gacgcttgcc attcacagcc
     5461 tctcatggcg aggaaatttc gcaggaagca ctcccggtat tccatccgta aagaaggcgg
     5521 tcagcatcaa acgcgccaca acggatcggt tatgggtgga ttcgcaaaca cgttattcgg
     5581 cattgatggc atgggttcga gacggattgg tcaacatcca gtttcaggaa agcgctccag
     5641 gagttctaac tttcgcgcct cttccaccgg gcgacgccat cgtctttctg tggtcgggag
     5701 gcgtctggga aacggcgtgc cttcaatcgg gcacttcgct cgactccgca ccatccgcgt
     5761 tcctccaggc tctggagctt gaggggatcc acgtatcctg cacggcaatc gaatgaggcg
     5821 ccgcatgatc caaaaagata caagagaaag acgacacctt acttgacaaa tcgacagccc
     5881 aactcacgtt ccaccggttg atatcgatac ccggctaccg cttcacggat acgatttcgg
     5941 aaggagacaa aattgcggtc tatcggggac aaagactctc tgatgggcag ccggcccgcg
     6001 ttcgagcgat tggcgggaga tttccagacc cgcgtgacgt cgcacggatg cgacgcgatc
     6061 acgaaatttc cagcgctcta gaagttcccg gcgtaattcg ctctcttggc ctgtttccac
     6121 accagggtgg tcttgccatc gtactcgaag acccgggtgc agtcgagctt gcgcgagcca
     6181 ccgagcaagg agtccattcc atcttcgact tcttgcgaat cggaatccag gtggcagaga
     6241 tactcgcgca ggttcacaaa aaaagaatcg ttcataagaa ccttcatcca ggtagcattc
     6301 tagtccaccc gaccacaggt cacgtctaca taactgactt tggcatttct tccacactcg
     6361 gtcgcgagaa cgcaggactc ttgacgccgg atcaaattga gggacaactt ccctacatcg
     6421 cgccagaaca aacaggcagg atgaatcgat ccattgatta tcgctctgat ttctattctc
     6481 ttggcatcac tctatttgag attctcacag gaagacgtcc ctttgaaagc aacgatccaa
     6541 tggagttcgt ccatttccac atcgcacgcg atcttcctca agcgggcaag gcaagatacg
     6601 gcgacgcagt gccggaaggc atacaacagg ttctcagtcg tctgactgca aagaatcccg
     6661 aagatagata caaaagtgca gccggtcttg cgcgcgatct tacagaatgt ctgaagcaac
     6721 tccaggaaaa aggtacgatc agcaacttca cag
//
LOCUS       DAOQJL010000163         6641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6449_length_6641_cov_4.597783, whole genome shotgun sequence.
ACCESSION   DAOQJL010000163 DAOQJL010000000
VERSION     DAOQJL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_6641_cov_4.597783"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLG78_08770"
     CDS             complement(<1..389)
                     /locus_tag="PLG78_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23247.1"
                     /translation="MKLRWRLEVGLASAVFRFFGLWSGAGRIMCGRLFGFLGRILVAK
                     VRKRTRANLRIAFPEASEARIDQLMRDAYCSIAKGSYDFYWSGRWTKEEVESRIQWEP
                     ESLKSFESAYRAGKGIVLVVGHLGNWE"
     gene            440..841
                     /locus_tag="PLG78_08775"
     CDS             440..841
                     /locus_tag="PLG78_08775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE23248.1"
                     /translation="MPGRDPSGGFGSGVDNEDGLQLKLTFDEDTKTAYGEYRCPAKFR
                     GEKESVHPGVLAVILDEIMARINEAMNFDTVTGELTIRYLQSATVDENLYLRGWFVKK
                     NRRVVENRAEIENEIGKIVARGKGKYIERED"
     gene            complement(838..2121)
                     /locus_tag="PLG78_08780"
     CDS             complement(838..2121)
                     /locus_tag="PLG78_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23249.1"
                     /translation="MTDYAELHSHLYGCLTLDDLRYLAGRNKPRWEIFLKSYESAYGR
                     SADIQDLMAPESDSRLRSYYHFKGPPDFPKFQSAFDLIIALSSTEPGELFEVASRVCA
                     REPAVHAEYRQLFSPMISVSEFEEKTGALAEAMEAACKNHPGKTFRLVMSLPRNPVTG
                     PVLFESVRTLKRAGKNIVGIDFCAQEEGYPPSLLSDLLSAIKSAEERYSILYHVGESF
                     RDKSVESAVRWVVEAALAGAHRLGHAAALGMDPDVFRGSTREELASERLDQIDFEIKN
                     YDSIREFILARPAPGSAAAMVPNKDQLSQEARDIRASGKKVVSVFYSPERTEFLRAFQ
                     DWAMSQVAATGAVVECCPASNQAIIGITRNHPLLRFLKSNVRCVIGADDPGIFDTDLE
                     KEFETVRKWGVSESEIAQLKKNSLEFTSEKLTSVD"
     gene            complement(2114..2317)
                     /locus_tag="PLG78_08785"
     CDS             complement(2114..2317)
                     /locus_tag="PLG78_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23250.1"
                     /translation="MNPEDLREFYITSEFLTRLSRRARYWSRDFILEQMDHFRSRHPD
                     YPELIDVLQLEIDIRDKKELQVD"
     gene            complement(2385..3761)
                     /locus_tag="PLG78_08790"
     CDS             complement(2385..3761)
                     /locus_tag="PLG78_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNE23251.1"
                     /translation="MHLIVFARAPELGRVKSRLAAAIGKDSALGIYETLLDHTLAVAA
                     HFDFSIYLEGESSLILPVLDRAGIREPYLKEQTGENLGDRMIAAILSELDLHDRVILV
                     GSDIPDLSTDVISQCDRLLLENDLIFGPAHDGGFYLLGVHKGADTEEILRSLFSGMEW
                     SHNSVLAELLARARDLEKNVTLGPVLQDLDNASDLANWLSENQGSLKSRIQKTVPDVR
                     VIIPVLNEAENLPVVIRGLTAVFPFRQIICVDNGSTDGSAEIARSLGAHVLESARGYG
                     NACLAGMDWVKNQGGADVILFADADCADKPQDAVSVVGAVLRGADFALVERIPEEEGA
                     LLPHARFGNWLSTSLIALFFGHSYRDLGPLRAIRFASLLSLEMEDRNFGWTVEMQIRA
                     VRRGLKIVEIPGGYRKRLYGASKVSATIMGSIRAGLIILRTVGREVWRDRGASWAKRR
                     KRNHHPSK"
     gene            complement(3762..4631)
                     /locus_tag="PLG78_08795"
     CDS             complement(3762..4631)
                     /locus_tag="PLG78_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23252.1"
                     /translation="MVDETLQTPETPDPQRKKRDPFQNVGPIVSSETVTGRRELATRS
                     DIKTIKKAGIRVHGRKVVTDDVKKLKSLSAAPIIRPDEKISFYVEPVRIEYYIPKESR
                     FAVEMRDLYIPLIDPVVLPEHEVLHAAMEKNRFVDLIEVMNEYPQTISGLLESYHEVL
                     NLYEHVSRAVKQAEATEPEQFRTAFYLMEVLLENEPTVASLEFMGDFVTYNLNWLVRT
                     MNALGVEFRATDRIVSYFIKLRNNYWEEHNLEYDERFEILASLFFDQAYPHRGTSMAE
                     DDFLFEMFDRQRK"
     gene            complement(4621..5916)
                     /locus_tag="PLG78_08800"
     CDS             complement(4621..5916)
                     /locus_tag="PLG78_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE23253.1"
                     /translation="MRVKVLFCFVLLPLALLASDRTADLLDSRGDSRRIGMWYVVQEK
                     RSDLLKPVAEILLTSEDVRDQKQVLNVFAAYGDDLETALPEWYVYVDHFLKSGRDEDL
                     LIQAMDLGVKWKEYRLMNAFARMAVHPMHRVRLHAFRSMAALRNDQIMPILLGLSNSG
                     RAIERMYALEGMQEYSDSRVAPFAEKLLQDPNRSVRIFAIPGYAAQPGSDGNSYAIAR
                     LFTQEGDEEVRERVIDLIATRKWGQHTTLVAQACSDPSSLVRAAAFRGARVFNLAGSV
                     SRQLEQEPEARLRSAGLDALAAIGNSGGGTGLAHILSSDENPALRLKAAALLGMYRER
                     MGLSSLHYNLRNDSSADVRAEAADALGDIGDPSSAQVLRTVMEDDQEQIAVKSAAMLS
                     FLKLPLADKKSVLLSIVDTIKDRAFARQLQRAALSFNGR"
     gene            complement(5906..6370)
                     /locus_tag="PLG78_08805"
     CDS             complement(5906..6370)
                     /locus_tag="PLG78_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE23254.1"
                     /translation="MPKPGISKIEQYITFSVGGEDYGVPVLEVLEIVRIENLIKVPHG
                     RDYLLGMMDIRGQVLPVIDLKTKLQMEAMDSHPERAIIMQIGARKIGLGVDSVSHVRS
                     FSSDNIDEGPPVLRSGSSRHIIGVGKSGDQFIVLMSLNKIFSPEEVEQFFAR"
     gene            complement(6401..>6641)
                     /locus_tag="PLG78_08810"
     CDS             complement(6401..>6641)
                     /locus_tag="PLG78_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23255.1"
                     /translation="PAEGFQIACVGTFALGYLHNLLGMAQFACLVGFFGLWSGAYDWL
                     VRDKSPFLPRICCMLGISGYAIWLETQYKIWGIFL"
BASE COUNT         1691 a         1777 c         1566 g         1607 t
ORIGIN      
        1 aactcccagt ttccgaggtg acccacaacc agaacgattc ctttgccagc tcgataggca
       61 gactcaaagg atttcagact ttctggttcc cattgaattc tagattccac ttcctctttt
      121 gtccagcggc cggaccagta gaagtcgtaa gatccttttg cgatggaaca ataggcgtcc
      181 ctcatcaact ggtcgatgcg agcctcggat gcttctggaa aagcgatgcg aaggttggcc
      241 cgtgtgcgct tccttacttt ggcgaccagg attctgccca gaaagccaaa cagtctgcca
      301 cacatgatgc gccctgcccc ggaccacaga ccgaaaaatc gaaatactgc ggacgcaagg
      361 cctacttcca gcctccagcg gagtttcacg ggtcacaaaa taaggattga cttgcagcca
      421 accacttatt tttagattca tgcctggacg cgatccaagc ggcggttttg gctccggcgt
      481 cgacaacgaa gacggactac agctcaagct cacctttgac gaagacacaa agactgcata
      541 cggcgaatat cgttgccctg caaagttccg cggggaaaaa gaatccgtac atcccggagt
      601 gctcgcagtc atcctggacg aaatcatggc cagaatcaac gaagccatga acttcgatac
      661 agtcacggga gagctcacca tccgctacct tcagtcggcc accgtggatg agaatctata
      721 cctgcgcggc tggtttgtaa aaaagaatcg cagagtggtt gaaaaccgcg ctgaaatcga
      781 aaatgaaatc ggcaagattg tagcgcgcgg caaaggaaag tacatcgaaa gagaagacta
      841 gtctacggaa gtcaacttct cggaagtgaa ctccaaagaa ttcttcttca actgcgcaat
      901 ctcagactcg ctcactcccc atttcctgac agtttcaaac tctttttcta aatccgtatc
      961 aaagattcct ggatcatctg caccgatcac acagcgaacg ttggatttca aaaatcgcag
     1021 aagcggatgg ttcctggtaa ttccaatgat tgcctggttg gatgcaggac agcattccac
     1081 gacagcaccc gtggccgcca cctgactcat agcccaatct tgaaaagcac gcagaaattc
     1141 agttctctca ggtgagtaaa acacagacac aactttcttt ccagatgccc gaatgtctcg
     1201 agcttcctgc gatagctggt ctttgtttgg aaccatggcc gccgcggagc ctggtgcggg
     1261 acgagcaaga atgaactcgc ggatcgaatc atagttctta atctcaaaat caatttgatc
     1321 caaacgctca gaagcaagtt cttctcgagt gcttccgcga aatacatcag gatccatacc
     1381 cagggcagca gcatgtccca gtctgtgagc gccggccaga gcggcctcca ctacccatcg
     1441 caccgcagac tccaccgatt tgtctctgaa actttctccc acgtggtaca agatcgaata
     1501 acgttcctct gctgatttga tcgcggacag cagatcggaa agaagagaag gagggtaccc
     1561 ttcttcctga gcgcaaaaat caatccccac aatattcttt ccagcgcgct tgagcgttct
     1621 gactgattca aacaaaacgg gacctgtaac tggatttctg ggcaacgaca tcacaagcct
     1681 gaacgtcttt ccaggatgat tcttgcaggc ggcctccatg gcttctgcaa gtgcacctgt
     1741 cttttcctcg aattctgaaa cagagatcat gggggaaaac agttgtctgt attctgcgtg
     1801 aaccgcaggt tctcttgcac agactctgga tgcgacttca aaaagttcac ccggctccgt
     1861 agaagaaaga gcaatgatca ggtcaaatgc agattgaaac tttggaaaat ccggtggtcc
     1921 tttgaagtga tagtaactgc gcagacgaga atcagactcc ggggccatca agtcctggat
     1981 atctgcgctt cttccgtaag cagactcata agatttgaga aatatttccc agcgcggttt
     2041 gttgcggccg gccaggtatc tgagatcgtc taacgttaaa caaccataga gatgagagtg
     2101 tagctccgca tagtcagtca acttgcaatt cctttttgtc ccgaatgtct atttcgagct
     2161 ggagtacatc tatcagctca gggtaatcag gatggcgaga acgaaagtga tccatttgtt
     2221 ctagaataaa atcacgcgac cagtacctgg ctcgcctgga aaggcgagtc aggaattcgg
     2281 aggtgatgta aaattcgcgc aggtcttccg gattcacttg tctttgtttt tctccttagc
     2341 cttgcgcttc ttttcttcga ggagcttcat ctcatcatga atgtctattt cgatggatgg
     2401 tggtttctct ttcttctctt cgcccatgat gccccccggt cgcgccatac ttcccgccca
     2461 acggttcgca ggatgatcag gcctgcccgg atcgaaccca tgattgtggc ggacactttg
     2521 cttgccccgt acagcctttt gcgatagcct ccgggaattt caacgatttt cagtccgcgt
     2581 ctgacagcgc gaatctgcat ttccacagtc cagccaaagt tacgatcctc catttccagg
     2641 ctcagcaatg aagcaaaacg aatcgcacga agtgggccca gatccctgta cgaatgccca
     2701 aaaaacagtg cgatcaaaga agtagagagc cagtttccaa atcgtgcgtg aggtagaagg
     2761 gcgccctctt cctctggaat tctttccaca agggcaaaat cggcgcctct aagaaccgcg
     2821 cccactacac tgactgcatc ctgtggtttg tccgcacagt ccgcatccgc gaatagaatc
     2881 acgtctgcac caccctggtt tttcacccag tccatgccag cgaggcaggc attcccatac
     2941 cccctggcac tttcgagcac atgagcgccc agactcctcg caatttccgc agatccatcc
     3001 gtactaccgt tgtccacaca gatgatttgt ctaaacggaa acacagcagt gagaccccga
     3061 atgactacgg gaagattctc tgcttcattg agaaccggga tgatgacgcg aacgtccgga
     3121 acggttttct gtatccggct tttcagagat ccctgattct ctgaaagcca gtttgccaga
     3181 tcactcgcat tgtccagatc ttgcaacact ggacccagtg ttacgttttt ttccagatcc
     3241 ctggcccgcg ccaaaagttc agctagtaca ctgttatgcg accactccat cccggaaaac
     3301 aggctgcgca gaatctcttc tgtatccgct cctttgtgca ctccgagcaa atagaatccg
     3361 ccgtcatgag caggcccgaa tatcagatca ttttcaagga gcaaacgatc gcactgagaa
     3421 atcacatcgg tagaaagatc aggaatgtca gagccaacca gaatcacacg atcgtgcagg
     3481 tcgagttcag atagaatggc cgcaatcatt ctatctccca gattctcacc cgtttgctct
     3541 tttaaatagg gctcgcgaat gcccgccctg tcgagaactg gcagaatgag agaagactct
     3601 ccctccagat aaatggagaa atcaaaatgt gcggcaaccg caagtgtgtg atccagaaga
     3661 gtttcgtaga ttcctagggc agagtctttt ccgatcgcgg ccgccagtct ggattttacg
     3721 cgcccgagtt ccggagcgcg cgcaaacaca atcagatgca attactttct ctggcgatcg
     3781 aacatttcaa aaaggaaatc gtcttctgcc atactggttc cgcgatgagg ataagcctgg
     3841 tcaaagaaga gagaggccag aatttcaaat cgttcatcgt attctagatt gtgctcttcc
     3901 cagtaattgt tgcgaagctt gataaaatag gaaacgattc tgtctgtggc gcgaaattca
     3961 acacccagag cattcatggt tcgcacaagc cagttgagat tgtacgtaac aaaatcaccc
     4021 ataaactcaa gggacgcaac cgtgggctca ttttccagga gaacttccat gaggtaaaac
     4081 gcggtgcgaa attgctcggg ctctgttgct tccgcctgct tgactgctct ggaaacatgt
     4141 tcgtatagat ttaatacttc gtggtaggat tcaagcaaac cggaaatcgt ctgcggatat
     4201 tcattcatca cttcaatgag atctacaaaa cgattcttct ccatggcggc atggagcact
     4261 tcatgctccg gcaaaaccac aggatcaatg agcggaatat agaggtctcg catttcgacg
     4321 gcgaagcgcg actctttggg tatgtagtat tcaatgcgga caggctccac gtagaagctg
     4381 atcttttcat ccggacgaat gatgggagcg gcagagagtg atttgagttt ctttacatcg
     4441 tccgtgacga cttttcttcc gtggacacgg atccctgctt tctttatggt cttgatatcg
     4501 gagcgagtgg ccagctctcg ccttccagtg accgtctcag aggatacgat gggccctaca
     4561 ttctggaatg gatctctctt tttcctctga ggatctggcg tttccggtgt ctgtagtgtt
     4621 tcatctacca ttgaaagaaa gagccgctct ctgcaattgc cgcgcaaaag cccggtcctt
     4681 gatcgtatct acgatcgaaa gcagcacgct ctttttatcg gccagtggaa gcttcaaaaa
     4741 cgacagcatg gcggcgcttt ttacagcgat ttgttcctga tcgtcttcca tcacggttct
     4801 cagaacctgc gcagaagaag gatctccaat gtcccccaga gcatccgccg cttccgcgcg
     4861 tacgtctgcg gaggaatcgt ttcgaaggtt ataatgcaaa gaagaaagac ccatcctttc
     4921 gcggtacatg ccaagcagtg cggccgcctt gagcctgagt gccggattct catcggaaga
     4981 aagaatgtgt gccaggccag ttccgccgcc ggaatttcca atcgcggcaa gagcatcgag
     5041 gccagcggac ctcaggcgcg cttctggttc ttgctccagc tgtctggaaa cggatccggc
     5101 cagattgaaa actcgcgcac cgcggaaagc ggccgctcga accagagaag atggatccga
     5161 acaagcctga gctaccagag ttgtatgctg gccccatttc ctggtggcga tcagatcgat
     5221 cactctctct ctgacttctt cgtccccttc ctgggtgaag agcctggcga tcgcgtacga
     5281 attgccgtcc gaacctggct gcgctgcata acctggaatg gcaaagatgc gcacactgcg
     5341 atttggatcc tgaagcaact tctccgcgaa aggagctacc ctgctatctg aatattcctg
     5401 cattccctcg agagcgtaca ttctttcaat ggcgcgacct gaattggaaa gcccgagcag
     5461 aatgggcatg atctgatcat ttcgcaatgc tgccatggag cgaaacgcgt gaagccgaac
     5521 ccggtgcatt ggatgcacag ccattcttgc aaacgcattc atcagtcgat attctttcca
     5581 ttttacaccc aggtccatag cctgaatcaa aagatcttca tctcttccgg atttcagaaa
     5641 atgatctaca tatacatacc attcaggaag agctgtttcc agatcatctc cgtacgcggc
     5701 aaacacattg agaacttgtt tctgatcacg gacatcttcg ctggtaagta ggatttctgc
     5761 gactggcttg agtaaatcgc ttctcttttc ttgaaccaca taccacattc caatcctgcg
     5821 gctgtctccc cggctgtcca ggaggtctgc agttctatcg gaggcaagta acgcgagtgg
     5881 aagaagaaca aaacagaaaa ggactttaac gcgcaaaaaa ctgctccact tcttctggtg
     5941 agaatatctt gttcagactc ataagaacga tgaactgatc tccgcttttt cccacaccga
     6001 tgatgtgcct ggagctgccg ctgcgaagta ctggaggacc ttcatctatg ttgtctgaag
     6061 aaaaacttct cacgtgcgaa acagaatcaa ccccgaggcc aatctttctt gcaccgatct
     6121 gcatgatgat ggctcgctct ggatgcgaat ccattgcttc catttgtaat tttgttttaa
     6181 gatcaatgac gggaagaact tgtccgcgaa tatccatcat tcccaggagg taatctctcc
     6241 cgtgtggaac tttgattaga ttttcaatgc gaacaatctc gagcacttcg agtacgggca
     6301 cgccgtaatc ttctccgcca acggagaatg tgatgtactg ctcgatcttg cttatgcccg
     6361 gctttggcat ggcacattgt ttccggcggg gctcgccttg ctacagaaaa ataccccaga
     6421 ttttgtactg tgtttccagc cagatcgcat agccggagat tccaagcatg cagcaaatcc
     6481 tgggaagaaa gggactcttg tcccgcacga gccagtcgta tgcgccagac cacagcccga
     6541 agaaaccaac cagacatgcg aactgagcca tgcccaggag attgtgcaaa tacccgagtg
     6601 cgaaagttcc cacacatgca atctgaaagc cctctgccgg g
//
LOCUS       DAOQJL010000164         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6467_length_6628_cov_4.530808, whole genome shotgun sequence.
ACCESSION   DAOQJL010000164 DAOQJL010000000
VERSION     DAOQJL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_6628_cov_4.530808"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLG78_08815"
     CDS             complement(<1..313)
                     /locus_tag="PLG78_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23256.1"
                     /translation="MDRSGFPIDLTQSRWRACSGFQSDFALGLPANANCIPVKSFPIL
                     LDRILPVPTGSGTHDWTLETHFSLESPGKRSVALYLPVIGENWEIFLNGVSVRREIHS
                     SA"
     gene            complement(409..1530)
                     /locus_tag="PLG78_08820"
     CDS             complement(409..1530)
                     /locus_tag="PLG78_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE23257.1"
                     /translation="MRLWLSIFILIVSFSCKEAPRVRKPAKVTALVLFKVGSVRAGGR
                     EITTGEIIQDHELISVGAQSILDLQVRETEASIAMRLRENTEVTLAGFVDPEGQLNIS
                     PEQSKGTLLTQVEKLGAKERMLVRMPSALASVRGTKFETAIDPGNMARVTVLDGKVAA
                     RTRITELEDSAAASQIPELAQLTGALDKTETIIESGKSATLDPKAKDQWLEDSGLGPL
                     LNSPEFKALAGKETVSPEQIASAQAASAKHFQSPESQTKLKSSLSRPPAPPTVESVPK
                     EEWDRKLKEYEELISIEQAKAKDQTAAGPAVKARNDAIQAQLLSRIEQIMGKASETLL
                     LKNGTRIRGVIYQNGADYLVLTAEGSKTFSDAEVSGIEF"
     gene            complement(1583..3421)
                     /gene="htpG"
                     /locus_tag="PLG78_08825"
     CDS             complement(1583..3421)
                     /gene="htpG"
                     /locus_tag="PLG78_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNE23258.1"
                     /translation="MSTLQKGKLSIQTGNILPVIKKWLYSEKEIYMRELVSNAFDAIT
                     KLRKVSLHEDIFGADDQDFAIDIHIDREKGILTIEDNGIGMTAEEIQKYISQIAFSGA
                     EEFVKKYEQKDGHGIIGHFGLGFYSAFMVAKRVEIDTRSYKPDAEAAYWTSEGGEEYE
                     SGPGSRTKRGTEIRLHMDDESKSHLDKLEVTELVRKYCDFLAVPVRVDGSQVNRKDAL
                     WSKQPNQVQKEEYVEFYKYLFPHQGDPLFHIHLNVDHPFRLQGILYFPRMLHELDVNK
                     NGVRIFCRNIFVSDEAQEILPKYLTMLQGVVDLPDLPLNVSRSYVQTDPEIRKIASHI
                     TKKVADRLNEEFTKNRTQYEQIWREIGPFVKYAMLSDEKFYDQAKASVLFEIAGQENK
                     FCTIEEYQLQNKERAQEKIYYASDLIQQAGPMQMLKEQGIEAVYLPQVIDTHFIQFLE
                     SKGIGVHFTRVDAELAEHIVDAGASSKIVDPDAKSSEDRVRELFTKALANPKVTIRIE
                     SLKNDQVPALILLPEFMRRMSEMAAVYKMQDAKMGEEHTLVLNLKHPLIQALSRPGIL
                     AGEGPSKQERVARAIYGLARLSQGAVQPEEIGKFTGETYELLASVI"
     gene            3788..6070
                     /locus_tag="PLG78_08830"
     CDS             3788..6070
                     /locus_tag="PLG78_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23259.1"
                     /translation="MLSATLLKTTLELLRDHLGTTGISYRAGGQVIYESGRIAGGGPV
                     AFMNGELFTGLFDKELSANERGAIRTAARMLEVFQGAAIPARGPNAISASLIAKSTFE
                     IAVSLEARSRQLAHYDKILQLNKSILLAQDLQGVLQILMDTAREALTGVGSSLLLVDP
                     RTGELYFNVVSGEKGGELKEIRIPPGKGIAGDVVQKAKAELIRDAVTDPRMFQEVDQK
                     LDQRTRDMVVAPLVARDRVIGVVEVINSQSPEGFTKEDLEFLVNIASHTSLLIDNAKN
                     RDDLIKSNHALDRKISELNAFSEVSRVLNSTLDSIEMRKGLLRTLLKLMHIGQGAIVI
                     PDPTGRRAVSEVRMRLKDGRLEEYGELTVYEDTADILLWMKQNREPLFFANSQGGETE
                     GLVRRFLSANASLEGEHLDLWFPVFQDDQQTVSFIVSLSDVSFRRKHPSDDLTFFKGI
                     MSLSYAAVRNVESYQNVIKAQEREERIRRGFQRYVPERVVEEVFAQEESPAPRSQKVS
                     VLFADIRNFTRHAENRDPGELLDLLNEFFEEMVEAVTHAGGTVDKFMGDSIMVLFGVP
                     DPTDVDAKSALTCARDMIGRLDVLNRRREESSRPTFQVGIGVHTGSAIIGNMGARKRM
                     DFTAVGDTINLGARLEQLTKLYQVRALFSEDTLKEAGDVTCREVDLIQVRGRMAVTRI
                     YQLATNREEDEMIRASAEVWPEMLKAYRMRNFWDCLQILISLPNDPLAGVFRARCESF
                     VKNPPPADWKGFYHLDEAWI"
     gene            6143..>6628
                     /locus_tag="PLG78_08835"
     CDS             6143..>6628
                     /locus_tag="PLG78_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNE23260.1"
                     /translation="MKKINIEQTFDHPLDVLLSARQERYKHLDKFPELKNVQIVSETT
                     EGHILKQVRNISLADAMPAVLQTVLPSGANMLVEESEFDDTTNTHTFKVTPGGGLDNI
                     FMVKGVSRYFASGDNKSTRTYDIEVTSKVLFVGGVVETAIGDVYRHNLEKDKSSIEHF
                     IQ"
BASE COUNT         1570 a         1612 c         1689 g         1757 t
ORIGIN      
        1 gagcagatga atggatttcg cggcgaacag atacgccgtt taaaaatatt tcccagtttt
       61 cgccgatcac aggcaaatac agtgcgacgc ttctcttgcc gggtgattcg agtgagaagt
      121 gagtttcgag agtccagtca tgagtcccag aacctgttgg aacgggaaga atgcggtcca
      181 gtaagattgg aaatgatttt acggggatgc agttcgcatt cgccggaagg cccagggcga
      241 agtccgattg aaatccgctg caggcgcgcc atcttgactg cgtcagatcg attgggaatc
      301 ctgaccggtc caaaggcgct gaacagcctg cggcaagcag gagtagaagg aatacgtttc
      361 gaatcatggc ccttttggat tgctctaacg ttatgcggga ttggttggtc aaaattcaat
      421 gccgctgact tctgcatcgc tgaatgtttt gcttccttcc gcagttagca cgagatagtc
      481 cgcaccgttc tggtaaatga ctccgcgaat tctggttcca tttttaagca gcagggtttc
      541 cgaagctttg cccatgatct gttcaatgcg cgagagaagc tgcgcctgta tcgcgtcgtt
      601 tcttgccttg actgcgggtc cagccgcagt ctggtctttt gcttttgcct gttcaatgga
      661 aataagttct tcgtattctt tgagcttgcg atcccattct tcttttggaa cagactctac
      721 tgtgggaggt gcaggcgggc ggctcaggga agatttcagt tttgtttgtg actctggact
      781 ctgaaagtgt ttcgcgctgg ccgcctgcgc gcttgcgatt tgctccggag aaacggtctc
      841 ttttccggcg agagctttga attccggaga attgagaagc ggccccaggc ccgagtcttc
      901 tagccactgg tcttttgctt ttggatcgag cgtggcgctc ttgccgcttt cgatgatggt
      961 ttctgttttg tccagggctc ccgtcagttg tgctagttct gggatttgcg acgcggcggc
     1021 cgaatcttcc agttctgtga ttctggtgcg ggctgcgact tttccgtcga gtacagtgac
     1081 gcgcgccata tttcctggat cgatcgctgt ttcgaattta gtgcccctta cagatgcaag
     1141 agcggaaggc attctcacga gcattctttc ctttgccccg agtttttcaa cctgagtgag
     1201 gagtgtgcct ttcgattgtt caggagaaat attcagctgg ccttccgggt ccacaaaccc
     1261 agccagagtg acttctgtat tttctcgcag acgcatggcg atggatgctt ctgtttcgcg
     1321 cacctggaga tccagaatgg actgagctcc cactgaaatg agttcatgat cctggatgat
     1381 ttccccggtt gtgatttcgc ggccccctgc ccgaacggag cccaccttga aaagaacaag
     1441 tgcagtaact ttcgcaggtt ttcggactct gggcgcttcc ttgcaagaga agctcacgat
     1501 cagaatgaaa atagaaagcc acagacgcat gggggactct attctcaaac actctggttt
     1561 acaatctaaa aatcgtcagg gctcagatga cagatgcaag aagttcatag gtttctcccg
     1621 tgaatttccc gatttcttct ggttgaaccg ctccctgcga taggcgcgcc agtccgtaga
     1681 tcgcgcgagc tactctctcc tgtttggaag gtccttctcc cgcaagaatt cctgggcgag
     1741 agagagcctg gatcaaggga tgcttgagat tcaaaacaag agtatgttct tctcccatct
     1801 tcgcatcctg catcttgtac accgctgcca tttcagacat tcgtctcatg aactctggaa
     1861 gcaggatcaa agcaggcacc tgatcgtttt taagcgattc gattcggatg gttactttag
     1921 gattggccag tgcctttgtg aatagttctc gcactctgtc ttcgctgctc tttgcgtccg
     1981 gatccacgat tttggaagag gcgcctgcat ctacgatgtg ttcggcgagc tctgcatcca
     2041 cgcgagtgaa gtgtacgcca attccttttg attctagaaa ttgaatgaaa tgagtgtcga
     2101 tcacctgcgg gagatacacc gcttcaatgc cctgctcttt cagcatttgc ataggcccgg
     2161 cctgctggat gagatcgctt gcgtagtaga tcttttcctg agctcgttct ttgttctgca
     2221 attgatattc ttcgatggtg cagaatttgt tttcctgtcc ggcgatttca aaaagaacag
     2281 atgcctttgc ctggtcgtag aatttctcat cgctgagcat ggcatatttt acaaacggac
     2341 caatttctct ccagatctgc tcatactgag ttctattttt cgtgaattct tcgttgagtc
     2401 tgtccgcaac tttcttcgtg atgtgagagg caatcttgcg aatttctgga tctgtttgca
     2461 cataagacct ggacacgttg agtggaaggt ctggcaaatc cacaacgccc tggagcatgg
     2521 tgagatactt tggaagaatt tcctgcgcct catccgatac gaaaatgttt ctacaaaaaa
     2581 tccgaacgcc gttcttgttt acatcgagtt catgcagcat ccgcgggaag tatagaattc
     2641 cttgcagccg aaatggatgg tccacgttca ggtggatgtg aaagagagga tcaccctggt
     2701 gaggaaataa atacttataa aattcaacgt actcttcttt ctgaacttga ttgggctgct
     2761 tgctccagag tgcgtctttg cggttcacct gggagccatc tactcttaca ggcaccgcca
     2821 gaaagtcgca atattttcgg accagttctg taacttccag tttgtctaaa tgcgatttgg
     2881 attcatcgtc catgtgaagg cgaatttcag ttccacgttt tgttcgcgac cctggcccgc
     2941 tttcgtactc ttcgcctcct tccgaagtcc agtatgccgc ctctgcgtcc ggcttgtaac
     3001 tgcgagtatc gatctccact cgttttgcaa ccatgaatgc tgagtagaat ccaagcccga
     3061 agtgcccgat gattccgtgg ccgtcctttt gttcgtattt ctttacgaat tcttccgcac
     3121 ccgagaacgc aatttgcgaa atgtattttt gaatctcttc ggctgtcatt ccgatcccat
     3181 tatcttcaat ggtcagaatt cccttttcgc gatcgatgtg aatgtctatg gcgaagtcct
     3241 gatcatccgc gccaaagata tcttcgtgaa gtgagacttt gcgaagcttg gtgattgcat
     3301 caaacgcgtt ggaaaccagt tcgcgcatgt agatttcttt ttcagagtag agccatttct
     3361 tgatgaccgg aagtatattg ccggtttgta tagaaagttt acctttttga agtgtggaca
     3421 tagttcctcc aattaagagg gccgtatgcc tttatgcagg actatttctg cggagggcaa
     3481 ggacacgcgc ggttgcactc gatcttaaca ccgcggcaga ttgccatgca gttgttcttg
     3541 agagtgccag tctctgtaca gacttcgccc gttggcaggt cgagacattg cccgcattga
     3601 tctgttttct tttctggttc ctgtggcgga gacttacaaa aagctccggc ggaaagggcc
     3661 agaaagatcg cacacaatcc tgcaagtttt ctcatacagc ccgattttag gaaatgagcg
     3721 gaaaggtccg ggtcaagccc ttttttttgg cgagggaagc actctgagat ccgttaattg
     3781 aagcagtatg ctttcggcaa cactgctcaa gacgaccctc gaactattgc gcgatcatct
     3841 gggaaccacc ggcatttcgt atcgcgctgg cggacaggtg atttatgaat ctggcagaat
     3901 cgccggtggc ggaccggttg ccttcatgaa cggtgagctc ttcaccggcc tgtttgacaa
     3961 ggagttgtcc gcaaatgagc gcggcgcgat tcgcacagca gcacgaatgc tcgaagtctt
     4021 tcaaggagcc gcgattcccg cgcgcggccc caatgcaatc tctgcttctc tcattgctaa
     4081 aagtacgttt gagattgccg tttcgcttga agcgcggtcc agacagctcg cgcactatga
     4141 taaaattctt caactaaaca agtccatcct tcttgctcag gatctgcagg gagtgcttca
     4201 gattctgatg gatactgcgc gcgaagcgct gaccggagtg ggatcttccc ttttgcttgt
     4261 ggacccgcgc accggagaac tgtacttcaa tgtagtatcc ggcgagaaag gtggtgaact
     4321 caaagagatt cgtattccgc cgggcaaagg gattgcgggt gatgtagtcc agaaggcaaa
     4381 agcagaattg atccgcgatg cagttacgga cccgcgtatg ttccaggaag tggatcagaa
     4441 attggatcag agaaccaggg acatggtggt cgcaccgctt gtggcccgcg accgcgtcat
     4501 tggcgtggta gaagtgatca acagtcagtc tccagaaggt ttcacaaagg aagatcttga
     4561 atttctcgtg aacatcgcat cgcatacatc ccttctgatt gacaatgcga aaaacagaga
     4621 cgatctgatt aaatccaatc acgccctcga tcgtaaaatt tctgaactaa atgcattcag
     4681 cgaagtcagt cgcgtactga actctaccct cgattccatt gaaatgagaa aagggctcct
     4741 tcgcaccctg ctcaaactca tgcatattgg ccagggcgcc attgtcattc cggatcccac
     4801 aggaagacgt gctgtttctg aagttcgcat gaggctcaaa gacggcagac tcgaagaata
     4861 tggtgaactt acagtctatg aagacacagc ggatatactg ctgtggatga agcaaaatcg
     4921 cgagcctttg ttctttgcca acagccaggg tggagaaaca gaaggactcg tgcggcgttt
     4981 cctgtccgca aacgcctctc tcgagggaga gcatttggat ttatggtttc ctgtttttca
     5041 ggatgatcag caaaccgttt ctttcattgt atctttgtct gatgtaagct ttcgtcgcaa
     5101 acatccatcg gatgatctca ctttcttcaa aggaatcatg agtctatcgt atgccgcagt
     5161 gcgaaatgtg gaatcctatc agaacgtaat caaggctcag gaacgcgaag aaaggattcg
     5221 ccggggattc cagcgatacg taccggaaag agttgtggaa gaggtattcg cgcaggaaga
     5281 atcacccgca ccgcgcagcc agaaagtaag tgttctattt gcggacattc gcaactttac
     5341 caggcatgcg gaaaatcgtg atccaggaga gctcctggac ctgctcaatg agttctttga
     5401 ggaaatggtc gaagcggtca ctcatgcagg tggcaccgtg gacaagttca tgggcgattc
     5461 gatcatggtg ctgttcggtg tacctgatcc aacggatgta gatgcgaaaa gcgcactcac
     5521 ctgtgcccgg gatatgattg gcagactgga cgtgttgaat agaagacgcg aagagtcctc
     5581 aagaccaact ttccaggttg ggattggcgt tcatacaggt tcagcgatca ttggaaacat
     5641 gggtgcgcga aagagaatgg atttcacagc agtgggagat acaatcaatc tgggcgcaag
     5701 actcgaacaa ttgaccaagc tctaccaggt tcgcgcgctt ttctctgaag acactctgaa
     5761 agaagcaggc gatgtcacct gtcgcgaagt cgacctgatc caggttcgag gcagaatggc
     5821 agtcacgcgc atctatcagc ttgcgacaaa ccgcgaagaa gacgaaatga tccgggcctc
     5881 tgcggaggtc tggccagaaa tgctcaaggc ctatcgcatg cgcaacttct gggactgcct
     5941 ccagatcctg atttcattgc ccaacgatcc gctggcaggc gtattcaggg ccaggtgcga
     6001 atctttcgta aagaatccgc cgccagccga ttggaaaggc ttctaccacc tggacgaagc
     6061 ctggatctga ttaacctgga attcttctga attcaagttg atctctggac gattccccca
     6121 caatggggcg caggattcct ccatgaaaaa gatcaacatt gagcaaacat tcgatcatcc
     6181 cctggacgtg cttctttcgg cgcgtcagga gagatacaag catcttgata agttccctga
     6241 attgaagaac gtccaaatcg taagcgagac aacagaagga cacattctta agcaggttcg
     6301 taacatttcc cttgcagacg caatgcctgc cgtccttcaa actgttctcc cttcaggcgc
     6361 caacatgctt gtggaagaat ctgaatttga tgataccaca aacacacaca ccttcaaagt
     6421 aactccgggt ggcggactcg ataacatctt catggtaaag ggagtctcca gatacttcgc
     6481 cagcggagat aacaagtcta cacgtactta cgacatcgaa gttacaagca aagtgctctt
     6541 cgttggtgga gtagtagaaa cagcaatcgg ggatgtgtat cgtcacaatc tagaaaaaga
     6601 caaatcgtcc attgagcatt tcatccag
//
LOCUS       DAOQJL010000165         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6468_length_6628_cov_4.497946, whole genome shotgun sequence.
ACCESSION   DAOQJL010000165 DAOQJL010000000
VERSION     DAOQJL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6468_length_6628_cov_4.497946"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..615
                     /locus_tag="PLG78_08840"
     CDS             187..615
                     /locus_tag="PLG78_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNE23261.1"
                     /translation="MSLSEDLYKEVILQHAGAPSHRGPLVGANVKESGVNRSCGDELE
                     LELIVKDGKIEKIALGGKGCSIHTASGSLMAEAVEGKSIAEALHIIRDFKSMLVDQKD
                     IHFPEDLEELEALRGVQRYPIRVKCATLSWNTLEQALNHK"
     gene            624..1646
                     /locus_tag="PLG78_08845"
     CDS             624..1646
                     /locus_tag="PLG78_08845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE23262.1"
                     /translation="MLPINVDFLGLHFRGYEGHWAFATLVLGFFYQRAKSLRAGYSND
                     WFVSAYTFAILTGFVCARLFHFMFWDTTNFLANPLIVFRPGGGFAILGGTIGTGFGGW
                     LYCKRTGANFYHWCESLMTPICLCLALGRISCFLNGDAYGLPSTLPWAVSFSEKSLDW
                     MAEWNGLHHLYANSDQPLAVISSIFQQYVPLSALPLPQSLQHLQAEGVHSVADLARFY
                     PPNALLSQSELAAKGLVPFPTVYPRVHPAQLYELGIMLVAYFFIMYAEKQEWARQRSF
                     FLFWICYGLNRFFIEMVRGDRNVAFASLTYAQVISILLVISGAGAFYYLTRKWKQTGL
                     PEPVLK"
     gene            1648..2304
                     /locus_tag="PLG78_08850"
     CDS             1648..2304
                     /locus_tag="PLG78_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE23263.1"
                     /translation="MANGSIVVGGTGLIGSEVLKLLAADPAFTPVTALARRSFDAPAG
                     IQVKVTDFSEKLESETPASEYYFCCIGTTIKKAGSQEAFRKVDYDLVINSARAAFAKG
                     ARIALVVSALGSDKNSSIFYNRVKGDMEEALQQIGFRTVYVFRPSLLTGDRAEMRPGE
                     KVGQAIGFLAGPLFLGPMKRYKPIAGKTVAAAMVRLAKAQGTGVHFIESEMIAKVASA
                     "
     gene            2327..3166
                     /locus_tag="PLG78_08855"
     CDS             2327..3166
                     /locus_tag="PLG78_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23264.1"
                     /translation="MIARICLLLALTPLTQCLTMQNIQAARGAPTDTRYKILGASVSD
                     EFAFLDVEGWQMHNTCSSDLDSSVHMPRGQNIPFTQCVSLKDGRAVDEEKCTKRKDFQ
                     VLEPWEKCCSNLLQISRNYYADLSSLNRTAKEGALSEYEKHRLEDISEVKVAPDLSVI
                     QIKEASGRLTTLYGQKSGDGMAFWPGALANNPRTPATVNLPIARRRTLQINGAKFLYP
                     EGDPGPDKFLRTTILTPQRPRPELWPFVPLMMAIDIVTLPFRILSEIGKFIVSPNKCY
                     PLF"
     gene            3439..4215
                     /locus_tag="PLG78_08860"
     CDS             3439..4215
                     /locus_tag="PLG78_08860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE23265.1"
                     /translation="MEKPFNTKIFLILQCAAVAGLLAVIPYALALNPAATPDKLPLPL
                     PLIAAIQIGIQALMFAAAIAVGLFFSSRIGLGLPLLEAKLKGEPIAPNVRAILPFSAV
                     AGIVSSVLIVALDVYVFQPALGGAIGVKLPGRANPAAWKGFLASFYGGINEEILLRLC
                     LLSFLAWVGGFLLRGQNGKPAISAFWTANVVAAILFGLGHLPATSMILPVTPLVVLRA
                     VVLNGLAGIAFGYLYFRFGLESAMLAHFIADIVLHVLLAL"
     gene            complement(4224..6263)
                     /locus_tag="PLG78_08865"
     CDS             complement(4224..6263)
                     /locus_tag="PLG78_08865"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE23266.1"
                     /translation="MSGQSTYVIWFATMDSLVVLLALIAILRLRSGLLRSIYLAFLAF
                     GALWVNLIYTVKWIAMPESIAVMLSRAIFVSILFTLCAFVCFMIAFCDLKEEFRKARF
                     FAFVGANALLILGTLLGYVENGVHMTATGLAPVYGPLHPVLVGSNLVWGAYVFWLVIR
                     EFRKSTDPIFRSQLRILFACCLFTFAWAGTMNGILPVLLGSSAFSHLGPLGFLVLYAG
                     IIWIILEERTLFIARDFRALLKLPAFQTQSNVMIMKQLFLDVEDAVTRPTSPFRKSYL
                     FHTGQGEADLTVSHGTGAAIQAPGALLPGNTEGFTDNLRALETQNRRLTFALLEAQSR
                     LRDPELGATIHSVPPRIPSSASGPYRLSDFVDEIEVNKRDMRESYGFPFPCFSRARVE
                     LLTRIRMYAPSLLTPLFVGPPASGRTTFSRVMHSLRSASSLREVSCASTDAGDLRPLV
                     REFLSPSCESTGILIRELHLLDPSGWPAIEQLLTEKRAGKWVYFTAEEEGLAELLSGA
                     GAHRATELLQLQLSVPALCSSREEMFFLALDLIRTRSRLLGRGIDAVSRTWMDRLISR
                     PWPGNYAEFAGVIDRALVESDSSIVDGSLPKLPAQATARKLEKAQAIPDLAHLTPLEN
                     AERRTIYEHLVANNFNQRKTSEEIGIRPNTLIAKMRKYEIAPPGGARPGRPRLNR"
     gene            complement(6260..>6628)
                     /locus_tag="PLG78_08870"
     CDS             complement(6260..>6628)
                     /locus_tag="PLG78_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HNE23267.1"
                     /translation="RGKGVEAINAHGPSDDLVDRVEADVLRDVFGARLHKIPAVSIKG
                     GIGSSMAAAGAMQVASAALMLKTGIIPPTRNYSNPDPDCALNISAQVRRMKRPLGSVL
                     VNAHGIGGMNSAILLKRVKV"
BASE COUNT         1672 a         1734 c         1643 g         1579 t
ORIGIN      
        1 acaggaaccg caagctgccc ctggactctt gtacctgcca atgataaata ccacgcaagg
       61 atcttcattc tagacgattt cgttcggagg ctagagcgag aactctctgc ctgagtgtga
      121 cattcgagaa tcagtcattc gagaatcagt tgccgaatgc gggcttaaga ttttgttaac
      181 tgtaggatgt cactgtccga ggacctttac aaagaagtaa ttttgcagca cgcgggcgcg
      241 ccctctcatc gcggtccgct tgtgggcgcc aatgtgaaag aatccggagt caatcgcagc
      301 tgcggagacg aactggagtt ggaactcatc gttaaggatg ggaaaataga aaaaatcgct
      361 ctgggtggaa aaggatgcag catccacact gcctctggat cgctcatggc ggaagcagtg
      421 gaaggaaagt cgatcgctga ggcattgcac atcatccgcg atttcaaatc tatgctcgta
      481 gatcaaaaag acatccactt cccggaagac ctggaggaac tcgaagcact gcgcggtgtg
      541 caaagatatc caataagagt aaaatgcgcc actctgtcct ggaacacgct cgaacaggcg
      601 ctcaatcata aataggcagg accatgcttc cgattaacgt agattttcta ggccttcatt
      661 ttcgtggcta tgaaggccac tgggctttcg ccactcttgt gctgggtttc ttctatcaaa
      721 gagcaaagtc tcttcgcgcg ggttattcca acgactggtt tgtatccgct tacacattcg
      781 ccatattgac tggatttgtt tgcgcgagac tctttcattt tatgttctgg gataccacca
      841 actttctcgc caatccgctg attgtttttc gcccgggtgg tggatttgcc attcttggag
      901 gaaccatcgg aacagggttc ggcgggtggc tctattgtaa gcgcaccggc gcaaactttt
      961 accactggtg cgaatcactc atgactccga tttgtttgtg cctggcgctc gggagaatca
     1021 gctgtttctt gaacggggac gcgtatggac tgccaagcac acttccatgg gcagtcagtt
     1081 tctcagaaaa gagtctggac tggatggcag agtggaatgg cctgcatcac ctgtatgcaa
     1141 acagcgatca accgcttgca gtgatttctt ccatattcca acaatatgtg ccgttatccg
     1201 ctcttcctct tcctcaatct ctgcagcatc tgcaagcgga aggcgttcat tccgtagcag
     1261 acctggctcg tttctatccg ccaaatgctc tcctctctca gtctgaactg gcggcaaagg
     1321 gtcttgtccc atttccaact gtgtatccgc gcgttcatcc tgcacaactc tacgaactgg
     1381 gcatcatgct ggtcgcctac ttcttcatca tgtacgcaga gaagcaagaa tgggccagac
     1441 agcgttcttt ctttttattc tggatctgtt acggactcaa tcgcttcttc attgaaatgg
     1501 ttcgcggaga cagaaacgtt gctttcgcat cgcttacata tgctcaggtg atttcgattc
     1561 tgctggtcat ctcaggggcc ggcgcgttct actatcttac aaggaaatgg aaacagaccg
     1621 gacttccaga gccggtgttg aaataatatg gcaaatggaa gcatcgtagt tggtggaact
     1681 ggactgattg gttccgaagt attgaaactc ctcgcagctg atcctgcgtt cacccccgtc
     1741 acagctctcg cgcgaagatc gtttgatgca cccgcgggca ttcaggtgaa ggtcacagac
     1801 ttttcagaaa agctggaatc ggagactccg gcctctgagt actatttttg ctgcattggc
     1861 actacgatca agaaggcagg atcccaggaa gcattccgta aggtagacta tgatctggtc
     1921 atcaattctg cgcgcgccgc atttgcgaaa ggtgcgcgaa ttgctctggt tgtttccgct
     1981 cttggttcag acaaaaactc gagcatcttc tataatcgag tcaaaggaga catggaagaa
     2041 gcgctccagc aaattggatt tcgcacagtc tatgtcttca gaccatccct ccttacagga
     2101 gatagagcag aaatgcgtcc cggggaaaaa gtaggacagg ccatcggatt ccttgcaggc
     2161 ccgctatttc ttggacccat gaagcgctac aagccaatcg cgggaaagac agtggccgca
     2221 gccatggtgc gccttgcgaa agcgcaagga accggcgttc atttcataga atccgaaatg
     2281 attgcaaagg tggcctccgc ctaacaacga gtaagcgatt gagcctgtga tcgcgcgcat
     2341 ttgtttactg ctagcactga ctcccctgac tcagtgcctg accatgcaaa acatccaggc
     2401 agcccgcggt gccccgactg acacgcgtta caagatacta ggcgcgagcg tttccgatga
     2461 attcgcattc ctcgatgtag agggctggca gatgcacaat acctgctcct ctgatctgga
     2521 ttcatccgtt cacatgccgc gcggccagaa tattcctttt acccaatgcg tttcgttgaa
     2581 agacgggcgc gcggtggacg aagagaagtg cacgaaacga aaagattttc aagtcctgga
     2641 accctgggaa aaatgctgct caaacctatt gcagatcagc cggaactact acgcagatct
     2701 ttccagcctg aaccgtacgg caaaagaggg ggctctttca gaatacgaaa aacatcgact
     2761 ggaagatatc tctgaagtca aggtagctcc agatttatct gtcattcaaa tcaaggaagc
     2821 ttccgggcga ctcaccactc tatacggtca aaaaagtggg gatggaatgg ctttctggcc
     2881 tggagcactt gcgaacaatc cgcgcacgcc ggcgacagtg aacctgccta ttgccaggcg
     2941 tagaaccctt cagatcaatg gggccaagtt tttgtatcca gaaggagatc cagggccaga
     3001 caaattctta cggacaacca tactgacgcc gcaacgaccg cgtccggaac tatggccatt
     3061 tgtgccgctc atgatggcta tcgacatagt cacgttgcca tttcgcattc tttcggaaat
     3121 tgggaaattt atcgtaagtc ctaacaagtg ttatcctctt ttctgaatga gaaggcaaga
     3181 tcgtctgacg ggttaccgtt aggcgatcta taaccaaaaa ctacggctgt ttctcgatct
     3241 tttcaccgct tcaccattac tttgatcacg ggatctctcc ttcttgagtt ttagaacgta
     3301 ctgatccaaa cacataagtt aagtgcttaa gtaagcaaaa ttctaaagtt aaaatccggg
     3361 cccatgggtc gcaattgagc ttgaccgaac agcaaaagaa caacgaatct tcgcaagagg
     3421 ataccgcgcg cccctgagat ggaaaagcct ttcaatacaa aaatctttct gatcctacag
     3481 tgcgccgccg ttgcaggatt gcttgcagtg attccttatg ctctggcttt aaaccccgcg
     3541 gccacgccgg ataaacttcc gcttcccttg cctctgatcg ccgcaattca aatcggcatt
     3601 caggcgctta tgttcgccgc tgccattgca gttgggctct ttttttcaag caggatcggg
     3661 ctcgggctcc cgttgctcga agccaaactc aaaggcgaac caatagcgcc taacgtcagg
     3721 gcgatccttc ccttcagcgc agttgcggga attgtctcct ccgtattgat cgtggccctg
     3781 gatgtgtacg tgttccagcc cgccctgggg ggcgccatcg gcgtgaaact tccaggacgt
     3841 gcaaatccgg cggcatggaa aggattcctc gcctcctttt acggaggcat caatgaggaa
     3901 atcctgctac gactctgtct gctttctttt ctggcctggg tgggtggatt tttactcagg
     3961 ggacagaacg gcaaacctgc gatttctgca ttctggaccg cgaatgtagt cgccgccatc
     4021 ctctttggac tcgggcattt gcctgcaaca tccatgattc ttcccgtaac gccattggtc
     4081 gttttacgag ccgttgtcct caatggatta gctggaattg cctttggcta tttgtatttt
     4141 cgcttcgggc ttgaatcggc catgctcgca catttcatcg cggacattgt actgcacgtc
     4201 ttgctcgctc tctaacatcg aggtcatcga ttgaggcgcg gtcgacccgg ccgcgcgcct
     4261 ccgggcggtg caatctcata ttttcgcatt ttcgcgatga gggtgttggg tctgattcca
     4321 atttcttcgc ttgtcttgcg ttgattgaaa ttgtttgcca ccaggtgctc ataaatggtg
     4381 cgacgttctg cgttctcaag cggtgtcaga tgcgccagat ccggaattgc ttgtgctttc
     4441 tcaagcttcc tggctgtcgc ttgcgcaggc aatttaggaa gggatccatc tacaatagag
     4501 gaatcgcttt caactaaggc acgatcgata acacccgcaa actcggcata gttgcctggc
     4561 catggcctgg agatcaaccg atccatccac gttcgactca cggcgtcaat gccgcgacca
     4621 agaagacgac ttcgagtgcg aatcaaatcg agcgccaaaa aaaacatctc ttctcgcgaa
     4681 gaacacaatg caggaactga aagctgcaat tgcaggagct ctgttgcgcg atgtgctccc
     4741 gcaccggaga gcagctcggc caatccttct tcctccgcgg tgaagtacac ccacttaccg
     4801 gcacgttttt cagttaacag ttgttcaatg gcaggccaac ccgaaggatc gagcagatgc
     4861 aattctcgaa tcagaatccc tgtactttca cacgaaggag aaagaaactc gcgcaccaat
     4921 ggacgaagat cgccggcatc cgtagacgcg caggaaactt cgcgcaatga tgaagcggat
     4981 cgcagggaat gcatcactct ggaaaaggta gttcttccgg acgccggtgg tccaacaaat
     5041 agaggagtca agagagacgg agcatacatg cgaatgcgcg tcagcagctc cacccttgcg
     5101 cgtgaaaaac aaggaaaagg aaatccatag gactcgcgca tgtcgcgctt gtttacttca
     5161 atttcatcaa caaaatcaga aagtctgtac ggacctgaag cggaagacgg tatgcgtggc
     5221 ggcacagaat gaatggtggc tcccagttcc ggatctctca atcgagactg agcttcgagc
     5281 aatgcgaacg tcaggcgtct gttctgagtc tcgagtgcac gtagattgtc cgtgaatccc
     5341 tccgtgttgc ccggcaacag cgcgccaggt gcttgaattg ctgctccagt tccgtgcgaa
     5401 acagtcaaat cagcttcacc ctgacccgtg tggaataaat agctttttcg aaacggagat
     5461 gtgggcctgg tgactgcgtc ctccacatcc aggaaaagct gcttcatgat catcacgttg
     5521 gattgagttt ggaaagcagg cagcttcaga agtgcgcgaa agtcgcgggc aatgaacaat
     5581 gtgcgttcct cgagaataat ccagataatg cctgcgtaaa gtacgaggaa tccaagcggg
     5641 ccaagatgcg aaaatgcaga cgaaccaaga agaacgggca gaatcccgtt cattgtcccg
     5701 gcccaggcaa atgtaaagag acagcaggca aacagaatcc tcagctgaga tcggaagatc
     5761 ggatctgtgc ttttcctgaa ttcacgaatc acaagccaga atacgtaggc cccccatacc
     5821 agattggatc cgacgagcac cggatgcaaa ggaccgtaaa caggagcaag tcccgtcgca
     5881 gtcatatgca ctccgttttc cacataaccg agcaatgtcc cgaggatcag aagggcattg
     5941 gcaccaacga aagcgaaaaa gcgtgccttc ctgaattctt ctttcaaatc acaaaacgcg
     6001 atcatgaagc acacaaaggc gcataacgtg aacaatatgg aaacaaagat tgcgcgtgaa
     6061 agcatgaccg cgatagattc cggcatcgca atccatttca cggtgtagat gagattcacc
     6121 cagagcgcac caaatgcaag aaacgcaagg taaatagatc gcagtaagcc tgatcgcaac
     6181 cttagaatcg caatcagtgc aagcaaaacg acgagtgagt ccatcgtcgc aaaccagatc
     6241 acgtaagtac tctgcccgct cataccttca ccctcttgag taaaattgcc gagttcatcc
     6301 ctccgattcc gtgcgcattt acgagcacag atcccagagg tcgtttcatc cgcctgactt
     6361 gcgccgaaat attcagtgca caatcaggat caggattaga ataatttctt gtgggaggga
     6421 tgatcccagt cttgagcatc aaagcagcgg aagccacctg catcgcaccc gcggcagcca
     6481 tgctgcttcc aattccacct ttgatggaaa cagccggaat cttgtgcaag cgagcaccaa
     6541 aaacatccct gagcacatct gcctctacac gatcgactag atcatcgctc ggtccatgag
     6601 cattgatcgc ttctacacct ttacctcg
//
LOCUS       DAOQJL010000166         6616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6481_length_6616_cov_4.227404, whole genome shotgun sequence.
ACCESSION   DAOQJL010000166 DAOQJL010000000
VERSION     DAOQJL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6481_length_6616_cov_4.227404"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLG78_08875"
     CDS             complement(<1..1390)
                     /locus_tag="PLG78_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE23268.1"
                     /translation="MLLIASVVLVFIFITAARTFGADWARVTSFGDFDLSGFRAHLKD
                     STSILFFQILAILGLSFLVARIVRRLGQPEVIGEMIAGIMLGPSLLGFLWPDAYTALF
                     PPASLGNLASLSQLGLMIFLFLIGMEVDLSHIRNHLRAAIFISHAAIVIPFTLGVLLA
                     LYIYRQYSPAGVDFLSFGLFIGISVSITAFPVLARIVQERGLLGSPTGRMALTVAAID
                     DATAWCILALVVALVQAKGVASAFVTVVLALTYVLVMLFVVRPFLNRMGTVYYTREII
                     GRGVMASIILLILGSAVLTQLIGIHALFGAFMAGVVLPADSRFGHLVAEKLRDFASVI
                     LLPLFFALTGVRTDFVLIFKSGAWDVMGLVLLVAIGGKVGGGMLSALLVGMKPKPALE
                     LGVLINTRGLIELIILNVGYDLGILTRELFAVLVLMAFITTFMTGPILSIMHRKRSED
                     VPVAVKPPSARGI"
     gene            complement(1540..2739)
                     /locus_tag="PLG78_08880"
     CDS             complement(1540..2739)
                     /locus_tag="PLG78_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23269.1"
                     /translation="MIQSSLRLSLFNLGLWFCLSYALLALVPPGSVNVFRVLFAAIFL
                     IANFFVARRLSWKEPGALRERSWLVFLLCFLSALILFFLRMSAHPHGMWDAWAAWNPK
                     AAFFAENFLGGNPFTVQLRLGSQPGYPLALPMSLGALGSMAGGWSPILPQLAHASFFL
                     VHWLLFFCIARRGVPAQPVWAGLCAALPALTFQASDQCADFPLSLALAGGTYLVTRPW
                     DSETPILDLWIAGICAGLLPNLKNEGLITGLAFLAFVFWSARSQKWPMRWVFVIVVPV
                     LILWASYFYLRAQSPEAPRFVGDLAAYLRDPARYIAVIKYDLLLLTVVTLLLPVALAM
                     RTRSYAALLPWVGLWAVYSAFFVITTYPQEWHLATAHFRLHLQFLPAFFVVLLLAPIR
                     GKAAENE"
     gene            complement(2736..3116)
                     /locus_tag="PLG78_08885"
     CDS             complement(2736..3116)
                     /locus_tag="PLG78_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23270.1"
                     /translation="MTSGRGVSVYLSLCLAFLLGLTWTAAGTIRADKHTRILQTAINE
                     MRSHRGIKAERVELCRSAHQIATEFSVAATYAMPGLSFVEVEKCAGPGHSIRFFLTDD
                     SQVSPAMEHQILFKGKGLVIVEAL"
     gene            complement(3113..3889)
                     /locus_tag="PLG78_08890"
     CDS             complement(3113..3889)
                     /locus_tag="PLG78_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE23271.1"
                     /translation="MAASNSRAPVSVLVPAFNEEATIGLVIDKLLKLPFVKEIIVVDD
                     CSKDRTAEIVEEKRRRNRKIRLLRHSENQGKTAAIKTAVQDAQGSIVIIQDADLEYSP
                     SEMINVVSPIINGVADVVYGSRFLVRREARVLYYGHFLANRFLTTLSNIFTNLNMTDI
                     ETGYKALRTPLLKSLVLTSSGFGMEAEITAHIARLPIRIYETPISYYGRTYEEGKKIG
                     MKDGIMAILYIFYYNILGVRLPSARKYRNTVRDFLSEIKR"
     gene            complement(3873..5033)
                     /locus_tag="PLG78_08895"
     CDS             complement(3873..5033)
                     /locus_tag="PLG78_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE23272.1"
                     /translation="MIASISGALRSHRFQVLLRYGMSGAVVFAFNMGLAAFLFSFQWT
                     SESDLHTNAANVLATEVSFIFSFFFHNFLTWRQRGPGIVRRFVHFHLVAAAGLAVRFI
                     LFALMIAFDASWLVSTLLSIGCQMFLNFVGFDRLVFSLAKQPLASAAEAYAADGAGVL
                     TLDTLEEAVNYNRYVLSKIAPSLGASNLELGAGRGTISALVAETHTVTLCELSQDNLR
                     ALRERFRGSSRVRAISEDFFRFKQREFDCIYSANVLEHIEDDERVLEHACELLTPGGR
                     FVAYVPAGMWLYSSFDRKIGHYRRYTRQDVRRLRSFVKERGLPLQLKWRYMNLPGALG
                     WFIRMRLLKASHIDRRSALVADRLVGLFAILDRLPLPLGQNMLLEFEYGRKQ"
     gene            complement(5030..>6616)
                     /locus_tag="PLG78_08900"
     CDS             complement(5030..>6616)
                     /locus_tag="PLG78_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23273.1"
                     /translation="EVAYFIQSLIFESGSRHALLIPPTGMALESLKDAAFLPFLIQTG
                     NEYYSAHMHGWSIVLSLFSMVHAKSIATLVFTLLNIGLFYALSRHFLSEAVASARIIA
                     LSLFVASPVLLFLSNTYMSHTLALALSQCVVLAHAKLTRLRPIWMVVFVLASVAAFFV
                     RPQSIVPLMAALVVYEMVRFACSEAKAASALRILFIVLSCGLAYGALSLYADWMRFDS
                     VIPMTAYVKQYLVDGCQSLGFGPGHGCFPTYGTFGHSFRKTIWNIGDLVARCSQELAV
                     GGVPLVLGVIVLIVRHFRQIRVDGPETLFLLILVITIGEFGLYFHNGGESYRGRYLAE
                     VAFALMLLVGLLIRHETILTSRAASVAAMGIAFALPVISLFQIRGEYFHPVLGSFSSV
                     GDRNPSIRDSLIRVEPVQGGVETVRSEFGGPDETIELTRRKRYLNLGYGTLAATAVRV
                     NANGLLEDARGNVLLGDISDEDAQRFGRPVYLLRYSAYEPVTKSSKIVRIWKRSPAVV
                     ERRGDSLLKSENSLKDRNGQ"
BASE COUNT         1980 a         1755 c         1597 g         1284 t
ORIGIN      
        1 gaattccgcg cgcagagggt ggcttaactg caacaggcac gtcctcggat cgcttgcgat
       61 gcataatgct gaggatagga ccagtcatga aggtagtgat gaacgccatt aataccagga
      121 ctgcgaaaag ctctctggtc agaattccga ggtcatagcc gacgttcagg atgatcagtt
      181 cgatgagacc gcgagtgttg atcaatacac cgagctcaag cgcaggcttt ggcttcattc
      241 cgacgagcaa tgcagaaagc attccgcctc ccacttttcc gccaatcgcc accagaagta
      301 caaggcccat tacgtcccag gcgccagatt taaaaatcaa aacgaaatca gtgcgaacac
      361 cagtcagtgc gaaaaacaaa ggaagtagaa tgacgcttgc gaaatcgcgc agtttctctg
      421 ccaccagatg accaaacctg gaatcggcag gaagcacaac gcccgccata aatgcaccaa
      481 aaagcgcatg aatgccaatc aattgagtca aaacagcaga acccagaatc aatagaatga
      541 tggatgccat tactccacgc ccaatgattt cgcgcgtata gtagacggtt cccattcgat
      601 tcaggaatgg cctgacaaca aacaacatca cgagcacgta ggtaagagcc agcactacag
      661 ttacaaaagc agatgcgact cctttcgcct ggaccagcgc aaccaccagc gcgagaatgc
      721 accaggcagt cgcgtcgtcg atggctgcca ctgtaagagc cattctgccg gtaggcgaac
      781 caagaaggcc ccgttcttga acaatgcgcg ccagaaccgg gaaagcagtg atgctcacgg
      841 aaatccctat gaacaaaccg aaagatagga aatccacacc tgctggcgaa tactgtctat
      901 agatgtaaag agcgagtaac acacctaagg tgaaggggat aacaatggcc gcatgactga
      961 tgaaaatagc ggcgcgaaga tgattgcgaa tgtgactcaa atcgacttcc attccgatga
     1021 gaaaaaggaa gatcatcaaa ccgagttgag atagactggc caggttccca agagatgccg
     1081 gaggaaagag ggccgtgtaa gcatccggcc acagaaatcc cagaagagac ggaccaagca
     1141 taatgccagc gatcatttca ccgatcactt caggctggcc cagacgtctg acgatgcgtg
     1201 caaccagaaa ggaaagtccg agaatcgcca gaatctgaaa gaaaagaatg ctcgttgagt
     1261 ctttcaggtg cgcccgaaac ccgctcagat caaaatcgcc aaaagaagta acgcgcgccc
     1321 agtctgcacc aaatgtccgg gcggcagtga taaagatgaa aaccagaaca acgcttgcga
     1381 tcagaagcat cagggcatat gcagtcaggt cacgccggcg agagttcaag tttgcctacc
     1441 tttgcgcatg gtttttgtct cgcagcggcc caacaagctt gatttgcgtc ccggacaaat
     1501 gccggttccg ataaccaatc ttatgtctca cccgcgcatc tattcgttct cagccgcctt
     1561 gccgcggata ggtgctagaa gcaagacgac gaagaacgct ggcaggaact gcaagtgaag
     1621 acgaaaatgc gccgttgcca gatgccattc ctgaggatac gttgtgatga caaagaatgc
     1681 agaatagaca gcccagaggc cgacccaggg aagcaatgcc gcgtaactgc gcgtccgcat
     1741 agccagcgca accggtagca aaagtgtaac tacagtgaga agaagcaaat cgtacttgat
     1801 caccgcgatg taccgagccg gatcccgaag ataggcggcc aggtccccca caaatcgcgg
     1861 tgcttccgga gactgagcgc gaagatagaa atacgaagcc cacagaatca gtacgggaac
     1921 cacgatgaca aaaacccaac gcatcggcca tttttgactc ctcgcactcc agaacacaaa
     1981 agcaaggaac gccagacccg taatcaaacc ttcattcttt agattcggca gcaaccctgc
     2041 gcagatgcct gcgatccaca gatcgagaat cggagtctca gaatcccagg gacgagtcac
     2101 caggtacgtg ccaccggcga gtgccagact caatgggaaa tcggcgcact gatcagacgc
     2161 ctgaaaagtc aaagctggaa gtgcagcaca caaaccggcc cacacaggtt gcgcaggaac
     2221 accacgacgc gcaatgcaaa agaacaataa ccagtgcacc aggaaaaaag aagcatgcgc
     2281 aagctgaggg agaatgggac tccaaccacc ggccatgcta ccgagagcgc cgagtgacat
     2341 tggcaacgcc agaggatagc caggctgcga tccaagccgg agttgaaccg taaatggatt
     2401 tccgccaagg aaattctccg cgaagaaagc agctttggga ttccacgctg cccatgcgtc
     2461 ccacattccg tgcggatgtg cagacattct aagaaaaaag agaatcagtg cggacaggaa
     2521 acaaagaaga aatacaagcc acgatcgttc gcgaagagcg ccgggttctt tccatgaaag
     2581 ccgcctggcc acaaagaagt tcgcgatcaa gaaaatagcg gcgaaaagaa cgcgaaagac
     2641 attcaccgac cctggaggaa ccagagccag aagtgcataa ctcaaacaaa accacagccc
     2701 caaattgaac agagagagcc tgagcgatga ctgaatcaca gggcctccac gatcaccagc
     2761 cccttccctt tgaaaagaat ctgatgttcc atggctggcg aaacctgtga atcatctgtg
     2821 agaaagaaac ggatcgaatg acccggaccc gcacactttt caacttccac aaaagatagg
     2881 cccggcatcg cataggttgc ggccactgag aactcggtgg caatttgatg cgcagatctg
     2941 cacaattcca ctcgttcagc tttgattccg cgatgactcc gcatttcatt gatcgccgtc
     3001 tgtagaatgc gtgtatgctt atccgctcga attgtcccgg ctgcagtcca ggtcaatcca
     3061 aggagaaagg ccaggcaaag actcaggtaa acggaaaccc cacggccaga ggtcatcttt
     3121 tgatctcaga aagaaaatcg cggacagtgt tcctgtattt gcgcgcagat gggagtctga
     3181 ctcctaaaat attataataa aaaatataaa gaattgccat gatcccatcc ttcatgccaa
     3241 tcttttttcc ttcttcgtac gtacggccat agtacgagat cggggtttcg taaatacgaa
     3301 ttggtaaacg cgcaatgtgt gccgtgattt ctgcttccat gccaaatccg ctactggtca
     3361 aaaccaggga cttgagcaaa ggagttcgca gggccttata acccgtttca atgtctgtca
     3421 tattgagatt cgtaaagata ttgctgagcg ttgttagaaa gcgatttgcc aaaaaatgac
     3481 cataataaag cacacgcgct tctcttcgca caagaaaccg acttccgtac acgacgtccg
     3541 caacgccatt gatgattggg ctgaccacat tgatcatttc tgaagggctg tactcgagat
     3601 ccgcgtcttg aatgatgaca atcgatcctt gcgcgtcttg aactgcggtt ttgatagccg
     3661 cggtcttccc ctgattctct gagtgtcgaa gtaaacgaat cttacgattg cgcctccgct
     3721 tctcctctac aatttcagct gttctgtctt tcgaacaatc atctactaca atgatttctt
     3781 ttacaaaagg aagcttcaga agcttgtcta ttacgaggcc aatcgttgct tcctcattga
     3841 atgccgggac cagcacactg accggggccc tgctattgct tgcggccata ttcaaactcc
     3901 agaagcatat tttgccccag cggcaaaggt agtcgatcaa gaatcgcgaa gagtcccaca
     3961 agcctgtcag ccacaagagc actccgccga tcaatgtgcg aagctttcaa taaacgcatt
     4021 ctgataaacc agccaagagc accgggtaaa ttcatatagc gccacttcaa ttgaagcggc
     4081 aaaccgcgtt ctttcacgaa cgagcgaagg cgtcgcacgt cctgccgcgt gtatctgcgg
     4141 tagtgaccaa tcttgcgatc aaaagaagaa taaagccaca tccccgcggg gacgtacgca
     4201 acaaaccgtc ctccgggagt gagcaactcg caggcatgtt cgagcactcg ctcgtcgtct
     4261 tcaatgtgtt ccagtacgtt ggcagaataa atgcaatcaa attctctctg cttgaaccga
     4321 aagaaatctt ccgaaatcgc gcgaacacgc gaggatccgc gaaacctttc gcgcagtgcg
     4381 cgcaaattgt cctgcgaaag ctcacagaga gtaaccgtat gcgtctctgc aactagagcc
     4441 gatatggttc ccctgcccgc accaagctca agattggaag cacccagtga aggcgcgatc
     4501 ttactcagta catatcgatt gtaatttaca gcttcttcga gggtatccag agtcagcacg
     4561 cctgctccgt ccgccgcata ggcctcagcg gctgaggcca gaggttgttt tgcaagcgaa
     4621 aacacaaggc gatcaaaccc aacaaaattc aagaacatct ggcagccaat agaaagaagc
     4681 gtacttacca accaggacgc atcaaatgcg atcattaatg cgaacaaaat gaaacgtacg
     4741 gccagtcccg ccgcagccac cagatgaaaa tgaacaaagc gccgaacaat cccaggccct
     4801 cgctgcctcc aggtcaaaaa gttatggaag aagaatgaaa atatgaaaga tacttctgtg
     4861 gccagaacgt tagccgcatt cgtgtgcaaa tccgattcag acgtccactg aaaagaaaaa
     4921 agaaacgcgg ccaggcccat gttgaatgca aagaccactg caccgctcat gccatagcga
     4981 aggagcacct gaaagcgatg agagcgcaag gcaccggaaa tactggcaat cattgcccgt
     5041 tccgatcctt taatgaattc tccgacttaa gaagcgaatc tcctctgcgc tccacaactg
     5101 cgggcgacct tttccatatt cgcacaatct tgctgctttt agtcacaggt tcataggcag
     5161 aatatcgcag cagatacacc ggccttccga atcgctgtgc gtcttcatct gaaatgtctc
     5221 cgagcaatac attgccgcgg gcatcttcca ggagcccatt tgcattcacg cgaacggcag
     5281 tagcggccag tgttccataa cccaggttca agtatcgttt ccttctagta agttcaatgg
     5341 tttcatccgg gccgccaaat tcagaccgaa ctgtctccac tcctccttgc actggttcaa
     5401 ctcgaatcag agagtcgcga atcgagggat ttctatcccc aaccgatgag aaagaaccca
     5461 ggactggatg aaaatactcc cctcgaatct ggaataagga aatcacgggc aatgcaaagg
     5521 cgattcccat tgcagcgacg ctcgctgcgc gcgaagtgag aatcgtctca tggcgaatca
     5581 ggagaccaac cagtagcatt agtgcaaaag caacttcggc caggtatctg ccgcgatagc
     5641 tttcgccgcc gttatgaaag taaagtccaa actcaccgat cgtaatgaca agtatcaaca
     5701 gaaacaatgt ttcgggtccg tccacgcgga tctggcgaaa gtggcgcacg atcagtacaa
     5761 tcacgcctaa caccagtgga acaccaccaa ccgcgagctc ctggctgcac ctggccacca
     5821 aatcacctat gttccagatt gttttgcgaa atgagtgacc aaacgttccg tacgttggga
     5881 agcatccatg cccggggcca aatccgagag attgacaacc gtccactaga tattgcttca
     5941 catacgctgt catcggaatc acggaatcaa atcgcatcca atccgcataa agagacaacg
     6001 cgccatatgc cagaccgcat gacaaaacaa tgaacagaat gcgcaaagca gacgccgcct
     6061 tcgcctcgct acatgcaaag cgaaccattt cataaactac gagggcagcc atgagcggga
     6121 caatggattg cggtcttaca aaaaatgcag ctacagacgc caaaacaaaa accaccatcc
     6181 agatgggcct taagcgagtt agcttcgcat gcgcaagcac aacacactgc gaaagagcaa
     6241 gagccagagt gtgcgacatg tatgtgttgg agagaaacag caagaccggt gaagcaacga
     6301 aaagggacag agcaatgatt cgcgcagaag caaccgcttc tgagagaaaa tgacgagaga
     6361 gcgcataaaa caggccaatg ttcagaagtg taaaaacaag agttgcgatg gactttgcgt
     6421 gaaccatgga aaacagactg agcacgatag accaaccgtg catgtgtgca ctgtagtatt
     6481 cgtttccagt ttggatcaag aaaggaagaa acgcggcgtc cttgagagac tcgagggcca
     6541 ttcccgttgg agggatcaga agtgcatgcc ttgaaccgct ttcgaaaatt agcgattgaa
     6601 taaaatacgc gacttc
//
LOCUS       DAOQJL010000167         6543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6590_length_6543_cov_3.912145, whole genome shotgun sequence.
ACCESSION   DAOQJL010000167 DAOQJL010000000
VERSION     DAOQJL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6590_length_6543_cov_3.912145"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLG78_08905"
     CDS             complement(<1..677)
                     /locus_tag="PLG78_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23274.1"
                     /translation="MKVTRWTICLIGFCSFCSSLSPGGPPAEITAITYNTHYMQRGVP
                     GLIRTLSPLQADVIAMQEVLVQNGHVSSQTIAQHLGMNHVSSLPYVAYGNSQWVLTIL
                     TRYPVLYVDQRLLGANRRALRVLLNVKGHRVNFVTMHLTPYTNEHPLKEANQIRSDLR
                     KREIKDLLEWIGTPPDPVVLLGDFNMLRGAVRLWGLNEYSLVTSAGYTDADGGFLPTN
                     SDTFPLD"
     gene            complement(797..1207)
                     /locus_tag="PLG78_08910"
     CDS             complement(797..1207)
                     /locus_tag="PLG78_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23275.1"
                     /translation="MRFFSIGLVLMFAHCATTLALRERWNGETVTNWEYNPPIQEKIP
                     IIYGGTRMDLRLLTAWKPLLCPVPEDSKTRGRLTHEWAPVAACAGTIDILPSFLLDTV
                     LLPVTIIWSLFEALTENAKTPATEYSRPENQSRD"
     gene            complement(1358..1807)
                     /locus_tag="PLG78_08915"
     CDS             complement(1358..1807)
                     /locus_tag="PLG78_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE23276.1"
                     /translation="MKERLLPRMHNVGIVVESLDNAISFFTELGLTLEGRAMIEGEWA
                     GRVTGLPSQQVEIAMMVTPDGHNRLELSRFLKPAVIADHRNAPVNSLGYLRVMFTVDN
                     LDNTLTKLEKIGSTLVGEVVDYENMYRLCYVRGPEGILIGLAEELQK"
     gene            complement(1840..2223)
                     /locus_tag="PLG78_08920"
     CDS             complement(1840..2223)
                     /locus_tag="PLG78_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE23277.1"
                     /translation="MPEFMLYIRNAKDAKKSLTEDQHLAFIKQCETYIGTLKSKNALL
                     AAQPIVREGVVLKKTEGGWNEKDIAADQETQVGYYHIRAASLEEAIQIAKENPEFDYV
                     PSATIEVRPVKTKEMETGFVYPTNS"
     gene            complement(2242..3060)
                     /locus_tag="PLG78_08925"
     CDS             complement(2242..3060)
                     /locus_tag="PLG78_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE23278.1"
                     /translation="MPETNVMRNSDNMSIAPDIVNTVQNRLTSIEVEHNVEILFAIES
                     GSRAWGFASEDSDYDVRFIYRHPIPWYLNILPKRDVIEYPIVDIMDYSGWDLRKAFFL
                     ANKSNPVLFEWLQSPIVYRKNDRFFDSFFPAAMAYFSPISTVYHYLHMATNNYREYLK
                     RDMVKLKKYFYVMRPIFSCMWVEERQTVPPMEFDVLRAAVVKDAVLTEIIEDLLTKKK
                     SGTELGLAPQIPELNAFLEERIAHFESVSAEFNPAQKPDAELLNNSFQSILGLR"
     gene            complement(3073..3786)
                     /locus_tag="PLG78_08930"
     CDS             complement(3073..3786)
                     /locus_tag="PLG78_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNE23279.1"
                     /translation="MIRFSIILSSFLALACQSFVPRPPQPEPRLVAVTGTASVRKAPD
                     IIYVEIMSFAESTDPRAAFESALKTSDTTVAHLRNSYNLATEDVAVGSLSISPAYKTI
                     KGERTDIIEKYKAQLLLHLSIRNFADAPKIISDVLKLGIGSVQSFRYDIDRRSEIVRA
                     AQVLAFEDARAKALALAKAAGFRSVSLITIEIDNSRAHFSLNDSVQGYTARNFESFQM
                     AKETLDVEAQVTCRFEMLP"
     gene            complement(3851..4342)
                     /locus_tag="PLG78_08935"
     CDS             complement(3851..4342)
                     /locus_tag="PLG78_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23280.1"
                     /translation="MKRIFHTVLASAALALAHGIHASDGQEPKKPVCPLIWIGSDEME
                     GNGNFFSARLKLEITGTEGAFHVEAWRCHGARATTPEDCRDLIVHSKALVRGRVSITG
                     QKVRFAGESVTEEIFWPEGMATGKYCLDTLDGTIEGSLFKAVHSDPCNPSTRVILKRV
                     DCT"
     gene            complement(4369..4995)
                     /locus_tag="PLG78_08940"
     CDS             complement(4369..4995)
                     /locus_tag="PLG78_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE23281.1"
                     /translation="MKDSIHKKPRQNLRERARAIKPFRCKNLIAVLEEPADYKNVGTV
                     IRNVNALGVEKAYVIDSRNLLPGDWAEMRDRKSLSATSASAIKWSFVRTFQSTDECIA
                     HLKKKGFVSMATSPHRKGRENIVLHEGNYTTPAKLAIWFGNESQGISELAVAASVACI
                     NIPMFGLIESLNLGTSTGIVLYEVTKQRRGYQSQYKRAGKRMPIPKKR"
     gene            complement(5007..5864)
                     /locus_tag="PLG78_08945"
     CDS             complement(5007..5864)
                     /locus_tag="PLG78_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23282.1"
                     /translation="MKRFLLLTSLLATAVYCQPPYLSSQEAFAVYATEFYGKKLPGTR
                     VTKLQNFSLSFQLPNKEPAILFLDNPFLACKSAPSDCETTLLKYLPSPDSADLKGDRL
                     SIRPMLKSKEYASQLQSMFNSGPKKQKAPTTPFIGDITILYAFDFPERMAVVDEEDMR
                     AVGASDVESLHRLALLNMEARTGELAYKEIQPESGIYALTYEDSLAPSRVLLQDKWAA
                     VARKIKGNPILAIPSREFVLFVAEGDAARVRSLRELVRKVFSGEDHAVSMQLMHWTPR
                     GWELYSDEQ"
     gene            complement(5897..>6543)
                     /locus_tag="PLG78_08950"
     CDS             complement(5897..>6543)
                     /locus_tag="PLG78_08950"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNE23283.1"
                     /translation="FMKIPFTTEQFLGVFAAYNQSVWPAQVALFAAALVILALSLKTF
                     SFSSIMSGSILGVLWIWMGIAYHIVQFTSINPAAYVFGIACILQGLIFLWNGFRRTLE
                     FRFSWDVRGWAGGLLILYALILYPIVGSLLGHVYPSSPTFGLPCPTTIFTFGMLLWVR
                     KPPLIVLIIPVLWSIVGFSAALQLGIKEDVGLPMAAALVIILQVFATKAPQPVR"
BASE COUNT         1588 a         1565 c         1693 g         1697 t
ORIGIN      
        1 tcgtccaggg gaaacgtgtc tgagttcgta ggaaggaagc cgccgtccgc atccgtgtag
       61 cctgcggaag taacaagcga atactcgttt agcccccaca gtcgcacggc acctcgaagc
      121 atgttgaaat cgccgagcaa tacaactgga tcgggagggg ttccgatcca ctcgagcaga
      181 tctttgattt cgcgctttcg caaatcggat cttatttgat ttgcttcttt gagtggatgt
      241 tcattggtat acggcgtgag gtgcatggtc acgaagttca ctctgtggcc tttcacatta
      301 agaagcactc gtaatgcgcg gcgatttgca ccgagcagtc tctggtcgac atagagaacg
      361 gggtagcgcg ttagaattgt cagaacccac tggctgttcc cgtaagctac gtatggcaga
      421 ctgctaacat gattcattcc aagatgttga gcaatggtct gcgatgaaac atgcccattt
      481 tgcacgagca cttcctgcat tgcgatgaca tctgcttgca aaggcgaaag agtccgaatg
      541 agccccggca cgcctctttg catgtagtgc gtattgtaag taatggccgt gatttccgcg
      601 ggggggccac ccggagacaa agatgagcag aaagagcaaa agccaatcaa gcagatggtc
      661 catctggtta ctttcatcga ggtccaaaat cggtcgccag gtagaagacg caaacagaaa
      721 gagctcctcc gatgagaaga attacgtgac cagaaaaaca aggatgtctt tcgcaagcgg
      781 ggtgcaaatg gcatggtcaa tccctggatt ggttttccgg tcttgagtat tcggtagcag
      841 gagttttcgc gttctcagtt agagcttcga acaggctcca gattatggta acaggcaaaa
      901 gcacagtatc cagaagaaag ctgggcagta tgtcgattgt tcccgcacag gcggcgacgg
      961 gagcccattc gtgcgtgagt cgaccgcgag ttttagaatc ttcggggacc gggcataaaa
     1021 gaggtttcca tgctgtaagc aggcgaagat ccatgcgagt acctccgtag ataataggaa
     1081 tcttctcctg gataggagga ttgtattccc agttggtgac agtctctccg ttccagcgtt
     1141 ctctcaaagc gagagtagta gcgcaatgcg cgaacatcag tacgagtcca atcgaaaaga
     1201 atctcacggc tctgctagaa gtgctttcaa ctgcgcgagc tcctctggct tcatactttt
     1261 gaagagaggc cggactgtaa agttaccata catcttgccg ccgcgcacct ttttgttctg
     1321 tcaagtcggt atcgtatttt cgcttaacgt tgctttctta tttctggagt tcttccgcca
     1381 gtccaatcag gattccctca gggccacgga catagcacag ccgatacatg ttttcgtaat
     1441 caactacttc tcccaccaga gtagacccaa tcttttcgag ttttgtcaga gtgttgtcca
     1501 ggttgtcgac tgtgaacatc acgcgcagat aacccagaga gtttaccgga gcatttctat
     1561 ggtctgcgat caccgctggt ttcagaaatc tggagagttc caggcgattg tggccatcgg
     1621 gagtgaccat cattgcaatt tcgacttgct gcgaaggaag ccccgtcact cgcccggccc
     1681 attctccttc gatcatggct ctgccttcga gtgtgagacc aagttcggta aaaaaggaaa
     1741 tggcattatc tagagattcg actacaatgc caacattgtg catgcgcggg agcaaccttt
     1801 ctttcatgtt tgccattctc aagtcaggag tagcacctat caactgtttg ttggatagac
     1861 aaatcctgtt tccatttctt ttgtcttcac aggtcttact tcaattgtag cagaagggac
     1921 gtagtcaaac tcaggatttt ctttcgcaat ttggatggct tcctccagac ttgcggcgcg
     1981 aatgtgatag tagcccacct gagtttcttg atcggctgcg atgtctttct cgttccagcc
     2041 accctccgtt ttttttagaa caactccctc gcgcacaata ggctgtgcgg ctagcagcgc
     2101 attctttgac ttcaacgtgc cgatatacgt ttcgcattgt ttgatgaacg caaggtgctg
     2161 gtcttctgta agagactttt ttgcgtcttt tgcgtttcga atatagagca tgaattcggg
     2221 cattgatttg ttccttcttt gctaccgtaa gcccaggatg ctctgaaagc tattgtttaa
     2281 aagttctgcg tctggttttt gggcaggatt gaattccgcg gagactgact caaaatgagc
     2341 aatgcgctct tccagaaagg cgtttagctc cgggatttga ggagcgaggc ccagttcggt
     2401 tccgctcttc tttttcgtga gcagatcttc aatgatttcc gtgagcactg cgtccttcac
     2461 tacagcggcg cgcaaaacgt cgaactccat gggaggaacg gtttgccgtt cttctaccca
     2521 catacacgag aagatgggcc tcatcacata gaaatatttc ttgagtttca ccatatcgcg
     2581 cttcaggtat tcgcgatagt tgttcgtcgc catgtgcagg taatgataga ctgtggaaat
     2641 cgggctgaag taggccatag ctgcgggaaa gaagctatca aagaagcgat cattcttgcg
     2701 atacacgatc ggtgattgca gccattcgaa aagcacaggg ttagacttgt ttgcaaggaa
     2761 gaaggcctta cgaagatccc agccgctgta gtccatgatg tcgacgatcg gatattcaat
     2821 cacgtctctc ttcggaagta tgttcagata ccacggaatg gggtggcgat aaatgaatcg
     2881 tacatcgtag tcgctatctt ccgacgcaaa tccccatgca cgagaaccgg actcgatggc
     2941 gaaaaggatt tcgacgttgt gctcgacttc aatggaagtc agtcgatttt gcactgtatt
     3001 cacaatatca ggtgcgatgc tcatgttgtc tgaattgcgc ataacgttag tttccggcat
     3061 cctgaggcca ttttacggga gcatttcaaa tcgacaggtt acctgggctt ccacgtcaag
     3121 agtttccttt gccatttgaa atgattcaaa gttgcgtgct gtgtaacctt gaacagaatc
     3181 attcagggag aaatgcgcgc ggctattgtc tatttcaatt gtgatgaggg aaacgctgcg
     3241 aaaaccagcc gcttttgcca gagcaagggc tttcgctcgc gcgtcttcaa aggcgagaac
     3301 ttgcgccgct cggactatct cagatctgcg atcaatgtca tatcgaaatg attgcacgga
     3361 gccaatcccg agtttcagca cgtccgaaat aatctttgga gcatctgcga aatttcggat
     3421 ggaaagatga agtaggagtt gcgctttgta tttttcgatg atatccgtcc gctcaccctt
     3481 gattgtcttg taagccggag aaatggaaag ggacccgacg gccacatcct ctgtggccag
     3541 attgtaactg tttctcaaat gagccactgt cgtgtcagat gttttgagcg cactctcgaa
     3601 tgcggcgcgc ggatctgtgc tttctgcgaa ggacatgatt tcgacataga tgatatcagg
     3661 cgcctttcgg acggacgcgg ttccagtcac tgcaacaaga cgcggttctg gttggggcgg
     3721 ccgcggcacg aatgactggc aggctagcgc aaggaaacta ctcaagatga tcgaaaatct
     3781 tatcattcat tttctccgat tctgggatat tctttgatcc tgtgtcgttt gattcgcacg
     3841 ccggagcgca ctatgtgcaa tctactcttt tcagaatcac tcgagtggac gggttgcatg
     3901 gatcggaatg aactgctttg aaaagacttc cttcgatggt tccgtcgagc gtgtccaggc
     3961 agtacttgcc cgtggccatt ccttcgggcc agaagatttc ttccgtgacc gattcacctg
     4021 caaagcgcac tttctgaccg gttatagaaa cgcgtccgcg aaccagagct tttgaatgaa
     4081 caataagatc tctgcagtct tcgggtgtgg tcgccctggc accgtgacat ctccaggctt
     4141 ccacatggaa ggcaccttct gttccggtta tttccagttt gagtcgagca gaaaagaaat
     4201 tgccgttccc ttccatttca tcggaaccga tccatatcaa agggcaaaca ggtttctttg
     4261 gttcctggcc atcgcttgcg tgaatgccgt gagcgagagc gagagcggct gaagccagaa
     4321 ctgtgtgaaa gattcttttc atgttgtctc ctgtggatgc aatggcgctt aacgtttctt
     4381 tggaataggc atgcgtttcc ccgctctttt atactggctc tggtaaccgc ggcgctgttt
     4441 cgttacttca tacaaaacga ttcccgtcga ggttccgaga ttcaggcttt caattagacc
     4501 aaacattgga atattgatgc acgcaacact tgccgcgacg gccaactcgc tgatgccctg
     4561 ggattcattt ccaaaccaga tcgcaagttt tgccggcgtt gtgtagtttc cctcgtggag
     4621 aacaatgttc tcacggcctt tacgatgtgg agatgtagcc atagaaacga agcccttttt
     4681 cttgagatgc gcgatgcatt cgtctgtact ctggaaggtg cggacaaagc tccatttgat
     4741 tgcggaggcg gaagtggccg acagcgattt tctgtctcgc atttcagccc aatcgccggg
     4801 cagcagattt ctggaatcaa ttacataggc cttctcaacg ccgagggcat tgacattgcg
     4861 aatgaccgta cccacattct tgtaatccgc aggttcctca agcactgcga tgagattctt
     4921 gcagcggaag ggcttgattg ctctggcgcg ttccctgaga ttttgccgcg gttttttatg
     4981 gatggaatct ttcataaatt atgaactcac tgttcatctg aatacaattc ccatcccctg
     5041 ggcgtccagt gcatcaattg catggaaaca gcatgatctt cgcctgaaaa caccttacgg
     5101 actagttcgc gcaaggatcg cacgcgcgct gcgtcgcctt ccgctacgaa caaaacgaat
     5161 tctcgcgaag gaatggcaag gatcggattg cctttgattt ttcgcgcaac ggcggcccac
     5221 ttatcttgaa ggagcactct ggacggagcc agtgaatcct catacgtaag tgcgtaaatc
     5281 ccggattcag gctggatttc tttgtaagcc agttcgcctg ttctggcttc catgttgagc
     5341 aacgcgaggc gatgcaggga ttctacatca ctcgcgccca ctgctcgcat gtcttcctcg
     5401 tcgactacag ccatgcgttc cggaaagtca aatgcataga gtatggtgat gtcgccgata
     5461 aacggtgtag tcggggcttt ttgctttttt gggcccgagt tgaacatgct ttgcagctgg
     5521 gatgcatatt ctttggattt tagcatgggg cggatggaca agcgatcacc cttcaagtcg
     5581 gctgaatccg gagaggggag atacttcaag agcgtagttt cgcaatccga gggtgcactc
     5641 ttacaggcca ggaatggatt gtccagaaag aggatggccg gttctttatt gggtaactgg
     5701 aaggagagcg aaaaattttg cagcttcgtt actctggttc caggcagttt ctttccgtag
     5761 aactccgttg cgtagacggc aaatgcttcc tgtgagctaa ggtaaggagg ctgacagtaa
     5821 actgcagttg caagaaggct cgtcagtaag agaaatcgtt tcatgtgata tcctatagag
     5881 tcaatgtcgc ttaacgttaa cggacgggtt gtggtgcctt cgttgcaaaa acctggagga
     5941 tgatcacgag cgctgcggcc attggcaatc caacgtcttc cttaattcca agttgcagcg
     6001 ctgcagaaaa gccgactata gaccagagta cgggaatgat caggacgatc aacggaggct
     6061 ttcgcaccca gaggagcatt ccgaaagtga agatggtcgt ggggcagggc aaaccaaacg
     6121 tgggcgagga tggatagacg tgcccgagaa gagagcccac gatcggatac agaatgagcg
     6181 catagagaat caagagacct ccggcccatc cccgaacatc ccatgagaag cgaaactcta
     6241 gagttctgcg aaatccattc cagagaaaga tcaagccctg aagtatgcag gcaatcccaa
     6301 agacgtaggc cgcaggatta atggaagtaa actgaacgat gtggtaggcg atccccatcc
     6361 agatccaaag gacaccgagt atggaaccac tcataatcga cgagaacgag aacgttttca
     6421 aagacagagc aaggatcacg agcgcggctg caaagagcgc cacctgtgcc ggccacacgc
     6481 tttgattgta agcggcgaac actccaagaa attgttcagt tgtgaatgga attttcataa
     6541 atc
//
LOCUS       DAOQJL010000168         6522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6619_length_6522_cov_4.385496, whole genome shotgun sequence.
ACCESSION   DAOQJL010000168 DAOQJL010000000
VERSION     DAOQJL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_6522_cov_4.385496"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /locus_tag="PLG78_08955"
     CDS             complement(<1..2346)
                     /locus_tag="PLG78_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE23284.1"
                     /translation="MKDFPEGRTLERTLLQNLGEGVYGVDQDGLCTFVNEAALAMLGF
                     SEAEVLKKDQHTLFHWRKEDGSPYPPEECPVSKTIRDLQTRHVEDSFVCKDGRMLPVE
                     LTIAPVSDEAGRPGAVVAFQDLTELRKARAEAKEAVEMRQVLLDQSLAGIALLKDRRI
                     LWANRRMETMFGYNTGGLHGLETSALYAANADFQTLGEQAYPTMQAGGTYSDELLMKR
                     KDGATFWCQLSGQLVNPREPARGSIWTISDVDENHRRAQELVESEERYRQAFEVNSAI
                     KLIIDEETGAIVDANGAACHFYGYSREQLLRMNIRDINCMSPDEITRELTAAGKQQRL
                     VFEFKHMLSSGEIRDVEVYSGPIRVDGRPRLHSIIHDITERKKAQSDLELAARVITGA
                     AEGVVVTDQHAKILAVNPAFTEITGFAQSEAIGQTPRILASGRQSQDFYQAMWEDLIA
                     KGNWAGEIWNRKKDGTVYPEWLSISRTGSGADLRYIAIFSDITRRKADEERIRRLAHY
                     DPLTGLPNRALLFDRAMQALSLCRRGDMPLALMFLDVDHFKRINDTLGHKIGDEILVA
                     LSQRLTGILRVEDTVCRLGGDEFVILLPEIGAEGAAHVATKLVESIQTPLNVSGGIAV
                     TLSVGITMFPEDGDDFEILCKNADTAMYRAKAEGRNGFRFYTAEMQKKAARHWELEHA
                     LRSALANGELAVHYQPQLSLDSSTVTGVEALLRWTHPQLGAVSPVEFIPIAEEIGLIT
                     TIGHWVLRCAVKQAVSWIQSGRESLVMSVNVSAAQLREAGLV"
     gene            2538..3578
                     /gene="dctP"
                     /locus_tag="PLG78_08960"
     CDS             2538..3578
                     /gene="dctP"
                     /locus_tag="PLG78_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNE23285.1"
                     /translation="MKQAVAYFFVFLIQSFALAAEPIEIRLASNLPDNSRMARDFKEW
                     SAKVEERTGRRISFRFYFDGSQGDERDVIRKTKLGQLDGGIITSGGLAMIHSDFRALR
                     APLFFASDAEHDYVVEKCTADFKRISSTAGFELVLWNDMGWVYWYSRQPIANANGLRQ
                     VKMWQWTEDTAISALHRQLGVSGVPLGQVELLSALQTGKVDAYYGTPLQSVEMDWYTQ
                     TRYSTSVPVAYEPGALMISNRVWNRISPADRNVILSLGSTLQAELRRDIRKWNDDATS
                     AMARRGVSVTQAESAFRSDLSEKAEVVYRTLSENAGSQTMYDLVKRHRSDYRRRHLSP
                     GRDKEPFVPQSR"
     gene            3671..4405
                     /locus_tag="PLG78_08965"
     CDS             3671..4405
                     /locus_tag="PLG78_08965"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNE23286.1"
                     /translation="MTKSLLLCLIWVSSLGAQTRELDRVLRTHVADGRVNYKALKDDP
                     LFERSVQRLAQVNPGTLKSQSERLAFWINVYNAFTLKLIVDHYPVASINDLHSGPGLI
                     VSTVIGKTVWKVWKFRIHERNYTLDEVEHSILRPMGDYRIHAAINCASRGCPPLRSEA
                     YTAEKLNEQLDDQMRCFLRPPYARYDSASNTLYVSKVFDWFAEDFEKQEPLPYALLPY
                     LPENVNPKTAASARIRTLDYDWNLNE"
     gene            4574..4789
                     /locus_tag="PLG78_08970"
     CDS             4574..4789
                     /locus_tag="PLG78_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23287.1"
                     /translation="MRFRFAVASSGCKVSAQTDNLPEISLFGLFGRTIILNKTWTGIQ
                     KSGTDQLTAALYSFGRNAIYLPRAQTS"
     gene            complement(4934..5347)
                     /locus_tag="PLG78_08975"
     CDS             complement(4934..5347)
                     /locus_tag="PLG78_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HNE23288.1"
                     /translation="MSRLTEKLSKAQQHAMSIRPKVGGFPVLAEVLRECGVEMNRWSL
                     PSCQSIYLMKEGSVVQQGTPLVTGTHEIPKFDRDALIAALRRDQRGESNFPEFLQSAW
                     NAGVTGYDVDFSARKITYYGASGENYLEEYPAVQL"
     gene            complement(5361..5831)
                     /locus_tag="PLG78_08980"
     CDS             complement(5361..5831)
                     /locus_tag="PLG78_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE23289.1"
                     /translation="MKNRQKPDPVQLKSHIGYHLRVISNAVSHSFARKLAATDVTVAE
                     CVILREMYAGNQKTSPGTVAEITGLTRGAVSKLVDRLLNKGLVTRTESSRDRRFQEIQ
                     LTPRAARLVPQLARIADENDESFFSILSQGEKKALVRTLVKLATIHRLNAHPIE"
     gene            6019..>6522
                     /locus_tag="PLG78_08985"
     CDS             6019..>6522
                     /locus_tag="PLG78_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23290.1"
                     /translation="MSSFRFVAIALVFVSQTSITPGPKKKEPEQLCRTYATEFEERPA
                     HTISTCQFDEAALEYRCTKSSGDFKTRVYASARDFVMEGIHRERAYAIRTRFRSDGKD
                     WEAKLVYTDDGYLAREENTALPHVVLVWDKWDASGRNTAGRMEVGKICSLVSSVEYKD
                     TDHASFAT"
BASE COUNT         1508 a         1660 c         1694 g         1660 t
ORIGIN      
        1 cacaagcccg gcttctcgca gctgggcagc agagacgtta accgacatta ctagactctc
       61 tctaccgctc tgtatccagg agactgcctg tttcaccgcg cagcgcagta cccaatggcc
      121 aatcgtcgta atcaggccga tttcctccgc gattggaatg aactcgaccg gagagactgc
      181 acccagctgt ggatgcgtcc agcgcaagag cgcttccact cccgtgaccg tgctggaatc
      241 cagggataac tgcggctggt agtgaactgc gagttcccca ttcgccagtg cgctgcgaag
      301 agcatgctcg agctcccagt gtcgcgccgc tttcttctgc atttcagcag tgtagaagcg
      361 aaaaccattt cgtccttctg ctttcgcgcg atacatggcc gtgtctgcgt tcttgcagag
      421 aatttcaaaa tcatcgccgt cttcgggaaa catagtgatg ccaacagaaa gagtcactgc
      481 aattccgccg gaaacgttaa gcggcgtttg gatgctctca acgagcttgg tcgctacgtg
      541 cgctgctcct tctgctccaa tttctgggag gagaatgaca aattcatctc ctcccagtct
      601 gcagacggtg tcctccactc gcagtatgcc agtgagtctc tggcttagag caacgagaat
      661 ctcgtcaccg atcttatgcc ccagcgtatc gttgatcctt ttgaagtgat ccacatcaag
      721 aaacataagt gccaggggca tatctccgcg ccggcacaac gagagtgcct gcattgcacg
      781 atcaaatagc agcgcacggt tgggtaaacc agtaagagga tcgtaatgtg ccagtcgtcg
      841 aatgcgttct tcatccgctt ttcgtcgcgt gatgtcagaa aagatggcga tgtacctcag
      901 gtctgcgcct gagccagtgc gactgatgga caaccactcg gggtaaacgg taccgtcttt
      961 cttgcgattc cagatttcac cggcccaatt tcctttcgcg atcaaatctt cccacattgc
     1021 ctgatagaaa tcctggctct gtctgcccga agcgagtatt ctgggtgtct ggccgattgc
     1081 ttcggattgt gcgaaaccgg tgatctcggt gaacgcggga ttgaccgcga gaattttggc
     1141 atgctggtct gtaactacaa cgccttccgc cgctcctgtg atgacgcgcg ctgccagttc
     1201 caggtcagac tgagctttct ttcgctctgt gatgtcgtga atgatagagt ggaggcgagg
     1261 ccgtccatcc acgcggattg gacccgaata cacttccaca tcccggattt caccagatga
     1321 cagcatgtgt ttgaactcga agacgagtct ctgttgtttt cctgctgcag tcaattctcg
     1381 cgtgatttcg tcgggggaca tgcaattgat atcgcgaatg ttcatgcgca gcaattgttc
     1441 ccgtgagtag ccatagaagt ggcatgctgc tccgttcgcg tcgacgatcg ccccggtttc
     1501 ctcgtctatg atgagtttga tggcactgtt gacttcgaat gcttgccgat agcgttcttc
     1561 gctttcgaca agttcctggg cgcgacggtg attttcatcg acgtctgaga tagtccagat
     1621 agatccacgc gctggttccc ggggattgac gagttgtccg ctcaactggc accagaaggt
     1681 cgcaccgtct ttgcgtttca tgaggagttc atctgaatag gttcctcccg cttgcatcgt
     1741 aggatatgcc tgctctccga gtgtctgaaa gtcagcgttt gcagcataga gagcggaggt
     1801 ttcaagtccg tgaagcccac cagtgttgta cccaaacatt gtttccattc ggcgatttgc
     1861 ccagaggatg cgacgatctt ttagaagcgc gatgcccgcg agactctgat cgagaagtac
     1921 ctgtcgcatt tccacagctt cttttgcttc cgctctcgct ttgcgcaact ctgtcaaatc
     1981 ctggaacgca accacggcgc ccgggcggcc tgcttcgtct gaaaccggag caatcgtaag
     2041 ttcgaccgga agcatccgac catccttgca cacaaaggaa tcttctacgt gccgagtttg
     2101 caaatcgcga atggttttgg acaccgggca ttcctccggt gggtagggac ttccgtcttc
     2161 ttttcgccaa tgaaacaagg tgtgttgatc ctttttcagc acttcagctt cagagaagcc
     2221 cagcatcgcg agtgctgctt cattcacaaa tgtgcacagt ccgtcttgat ctacaccata
     2281 cacgccttcg ccgaggttct ggagaagggt tcgttccagc gtgcgacctt cagggaaatc
     2341 cttcatcggg ttctgaccat ttttcggcca tggttgcgaa tgctcggaaa caatgctagc
     2401 gcgaatgact ttcgcgcatc cgtgtgcgaa agagcgtgtc ctgaattgag aatcaagatc
     2461 gattccgaag agattcctct cctgtaattc gtttgttttt tcgtgacgcg aaagcgtgct
     2521 aaacgtctga gcaaatcatg aagcaagccg ttgcgtactt tttcgtattt ttgattcaat
     2581 cttttgcatt ggcggccgag ccaatcgaga ttcgcctggc gtccaacctt cccgacaact
     2641 ctcgaatggc cagggatttt aaagaatgga gtgcaaaggt tgaagaacga acaggcaggc
     2701 gaatctcctt cagattctat tttgatggtt ctcagggaga tgagcgagat gtgatcagga
     2761 agacaaaact gggtcaactg gatggaggaa tcataacgtc aggcggcctt gctatgatcc
     2821 attctgattt tcgtgcattg cgtgcgccac ttttcttcgc aagcgacgcc gagcatgact
     2881 acgtggtgga aaagtgcacc gccgatttta aacgaatttc cagtactgcc ggcttcgagt
     2941 tggttctgtg gaacgatatg ggatgggtct actggtattc tcgccagcca atcgcaaacg
     3001 ccaacggatt gcgccaggtg aagatgtggc aatggacgga ggatacagcg attagcgctt
     3061 tgcacagaca actgggagta agtggcgtcc ctctgggtca ggtggagctt ctgtccgcac
     3121 tccagaccgg taaggtcgat gcgtactacg gcactcctct tcaatcagtg gaaatggatt
     3181 ggtatacaca gaccagatat tcgaccagtg ttcccgttgc gtatgagcct ggagccctga
     3241 tgatttcgaa tagagtttgg aatcgtattt cgcctgcgga tcgcaatgtg atcctctctc
     3301 tgggttcaac tcttcaggcc gagttgcgcc gtgatattcg taagtggaat gacgacgcga
     3361 catctgcgat ggcccgccgc ggtgtgagcg tcacgcaagc agaatctgca tttcgttctg
     3421 atctttcgga gaaggcggag gttgtgtatc gcacgctttc cgaaaacgca ggatcgcaga
     3481 ccatgtatga tcttgtgaaa cggcacagaa gcgactatcg caggagacat ttgtctccgg
     3541 gaagagacaa agaacccttt gtgccacaat cgcgctagcg tcctggaaac acgaatcatt
     3601 ggagtaaaaa tgtttaccct ttgcagtgct ggtatcttct tgagtgggga aagaagacag
     3661 gatgactgcc atgacaaaaa gcttgttgct ctgtctgatc tgggtctcat cgctcggcgc
     3721 gcaaacccgc gaacttgacc gggtgcttcg tactcacgta gcggatggcc gcgtcaacta
     3781 caaagctctc aaagacgacc cgctcttcga gcgatctgtg cagaggctgg cgcaggtaaa
     3841 cccgggtaca ctcaaatcgc agagcgagcg gctcgcgttc tggatcaatg tgtacaatgc
     3901 attcacactg aagctgatcg tagaccatta cccggtcgca agcatcaatg atctgcacag
     3961 tgggcccggc ctcattgtct ctacggtcat tgggaaaaca gtttggaagg tctggaagtt
     4021 caggatccac gaaagaaatt acactctcga cgaagtggag cactcgattc tcagacccat
     4081 gggcgactat cggatccatg ctgccatcaa ttgcgcatca cgcggttgtc cgcctctgcg
     4141 ttcggaggca tacacagccg agaaactaaa tgaacaactc gacgaccaga tgcgctgttt
     4201 tctcaggcca ccttacgccc gttacgacag tgcgtccaat acgttgtatg tttcaaaagt
     4261 ctttgattgg tttgcggagg attttgagaa acaggaacct ttgccgtatg cactattgcc
     4321 atatctgcca gaaaacgtga atccgaaaac ggcagcctct gcgcgcattc gcactctgga
     4381 ctacgactgg aatttgaatg agtaggaatt tccctctaac cgcactgaat gatcgactaa
     4441 cggtggttaa aaaacagaaa cctgctgacg acgaactgtt gttatgggag caagtttgca
     4501 aaaaccgcag atcaaaaacg attgcccaaa ccagtcttcc ctctttccct ggatgaatgc
     4561 gaaccccgct tgtgtgcgct ttcggtttgc tgttgcatcc agcgggtgca aggtcagtgc
     4621 tcaaacagac aacttacctg agatttctct cttcggattg tttgggagaa ccattattct
     4681 caacaagacc tggactggaa ttcagaaaag cggaacagat caactaaccg ccgctctata
     4741 cagcttcggc agaaacgcaa tctacttgcc tcgcgcgcaa accagctgaa tctagctctg
     4801 ggtttttttg gtgcgatcta tcttaccaat gtggcagaag cgctggtctt tcgccaccaa
     4861 tcattgcaat cagacatcgc gccttctgat cagcttcgtg ttgcatattc ttttcagttt
     4921 tgagagcggc gcttcacaat tgaacagccg ggtattcttc cagatagttt tcgccgcttg
     4981 cgccgtaata ggtgattttg cgtgcggaaa aatcgacatc atagccagta actcctgcgt
     5041 tccatgcact ctgtagaaat tcgggaaagt tgctttctcc cctttgatct ctcctgagag
     5101 ctgcaatcaa tgcgtcgcga tcaaattttg ggatttcatg cgttcctgta accaggggag
     5161 taccttgctg cacgactgaa ccttctttca tcaggtaaat ggattggcag gatggcaaag
     5221 accatcggtt catttctaca ccgcactcgc gaagaacttc cgcgagtacg ggaaatcctc
     5281 ctacctttgg gcgaatcgac atcgcatgtt gttgtgcttt gcttagtttt tcagtgagcc
     5341 gactcatgta atactccttg ctattcgatt ggatgggcgt tcagtctgtg aattgttgcg
     5401 agcttgacaa gcgttcggac cagagctttt ttttcgccct gcgagaggat tgaaaagaag
     5461 ctctcatcgt tctcatctgc gatgcgcgcc agctgtggca cgagccgggc ggcccttggt
     5521 gtaagctgga tttcctggaa gcgcctatct ctggaagatt cggtgcgcgt cacaaggcct
     5581 ttgttcagga gtcgatccac cagtttggag acagctccgc gcgtaaggcc tgtgatctcc
     5641 gcgaccgttc caggagaagt cttctgattt cccgcataca tttctcgcag aatcacgcac
     5701 tctgctactg ttacgtccgt ggctgccagc ttgcgtgcga atgaatgcga aacagcattc
     5761 gagatcactc tcaggtgata gccaatgtga gacttcaatt gaaccgggtc aggtttttgc
     5821 ctgttcttca ttcgtcgatt tccgtgttca taatagtttc ctaggaaata gttgtcaagg
     5881 attcaaatgg ggctggcctt cagatccatt caatcaaaaa ggcttgcgaa gtgccacaca
     5941 gggagaactc tcgggatgat ggttgttcaa tggattttca gacagcccgc caacaaaagg
     6001 cttcaggcgg agtatgcaat gtcctcattc agatttgtag cgattgctct ggtgtttgtt
     6061 tcgcagacat cgattacacc gggcccgaag aaaaaggagc cagagcaatt gtgccgaacg
     6121 tatgcgactg agtttgaaga acgtccagca cacacgatca gcacatgtca gtttgatgaa
     6181 gctgccctcg agtatagatg caccaaatcc agtggcgatt tcaagaccag ggtctatgct
     6241 agcgcgcgcg atttcgtaat ggaagggatt cacagagagc gagcctatgc gataagaacc
     6301 agattccgca gcgatgggaa ggattgggaa gcgaagctag tttacacgga cgatgggtat
     6361 ctcgcgagag aagagaatac tgcgctcccc catgtggttc ttgtctggga caaatgggat
     6421 gcgagtggtc gcaatacagc aggccgaatg gaagtaggta agatctgcag tctcgtaagt
     6481 tcggtcgagt acaaagatac cgatcatgca tcatttgcta ct
//
LOCUS       DAOQJL010000169         6517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6625_length_6517_cov_4.070102, whole genome shotgun sequence.
ACCESSION   DAOQJL010000169 DAOQJL010000000
VERSION     DAOQJL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6625_length_6517_cov_4.070102"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLG78_08990"
     CDS             complement(<1..775)
                     /locus_tag="PLG78_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23291.1"
                     /translation="MGGGYYSQDIAREARSSGGGVFSYQGYGVNAADASARREVHPLL
                     NVHGKNRECMNETAIVVALDVTRSRGNDTKVVYEKLPLFLGQIELKGYVKGAAISFAA
                     IGDANSDQAPLQVGQFEADNRLDEVLSKFWIEEGGGGTGQESYELAAYYYAKHSILDC
                     NRKGKKGFFFFVGDEGFYPSVSPDHVEKILGRSISGPVPSLQAFQALQEKYHVFFVQP
                     EKTWEERKADVDAEIQARVEAAGGQYKNVGVRASLIWNNR"
     gene            complement(779..1831)
                     /locus_tag="PLG78_08995"
     CDS             complement(779..1831)
                     /locus_tag="PLG78_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE23292.1"
                     /translation="MLELLGAAASAIGSIASQPWAIALAGIASAVGIFLVKHFLTRAG
                     DDMYHWLRRKIFGRRKVESETQRELASRETRTARERFSPFEVETPVDRYSVTEYVARG
                     DIASIYGGTNRSDRQVILKVAMSTGDNDLLQNEANTLRALHQGDTVYNKHLPELLDQF
                     RASSGEIGLVLARIRASDLRTVRESYPQGIPQQHILWILRRTLAAVGHAHSRGIIHGN
                     IDPSHILISAPDHNVFVIDWAYAVHKPAETGQGFKAINDHFSPPEVAEKKQPLPASDL
                     YSIGKCMIYLLGGNVQLDTMPDNVDERIQRFIKFFTRTSALQRAQDAWQMYAKADDLR
                     EEVFGPHKFLEFKIQE"
     gene            complement(1878..2453)
                     /locus_tag="PLG78_09000"
     CDS             complement(1878..2453)
                     /locus_tag="PLG78_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNE23293.1"
                     /translation="MSELRFRTLSQAEEHLERIEKAPAYKATGLWSEAQIFDHLANSF
                     ENSVSGYPGLLPAVLRKTIGRMIFRSQQKNGFMKPGSPNPNAPKVRAEGDAKQALKRL
                     RKAIVQFKAHDGEMAIHPAFDRLSKDEFSHLHAMHMANHLEHIHLEETKGRDARTARL
                     TGKTQSGKSGSRKSTGKRSASKKTGRSAKRK"
     gene            complement(2542..5361)
                     /locus_tag="PLG78_09005"
     CDS             complement(2542..5361)
                     /locus_tag="PLG78_09005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE23294.1"
                     /translation="MIVEFRKAAFVFIVAGVTTLAALLAERPEVPAEVRSWLPRINDL
                     APDLPGFPWPGAKNSPSDHRTAPGEATSRDLKTGGETKTGVPQKEKVLLENEKALEGV
                     LRTLGRKDRPVRIVHSGDSLMWADNVSSRLRRHLQSRYGDGGRGIVSIVNSPDSVVQD
                     IRIEPTGFSLQKISGAFPQGVDSKLGFFSRSSQSIADPANVQFSFPDGSSPIHKARVV
                     WSGNGTVNLASNGKTLDTATKVVEGACRLTVAETGAEGVHAITMQASGLPVIHAAIFE
                     SSSGVSYSALVTMGIHQAWVRTVSDENFICGWRAAAPDLVIFQFGLNESASIDTYYNG
                     FSQSVYETDLRAYYTRLRKALPDAAVLIVGPLDRVKSADGGLVPVAAQTQVREVQRKI
                     AREFGFAFYDTAVALGPSHVSEFMAKGFFLSDYMHLSVSGGNAVADAMIRDIFAGDSP
                     PEKASTVVEKKSEDRTILFHSVRYAWYLGGVILMAGLLRRFVRLRMYVLMAASMYFYA
                     SWRLWALGLLVLTATVDYILARGIENKRLEDTESSRRWAGFLLFLSLSSNLGILFFFK
                     YYNFFASSAVQAAGWFGLHADFPVLSLILPVGISFYTFQSLSYTIDVYRGVEPAEKSL
                     PRLLFFVSFFPQLVAGPIVRARHFLLRLREDMRHLTPSMRQFQTGIFLIMKGLTKKLA
                     ADWIAVQMVDRVYQNPGMFTSAEILAAVYGYGLQIYGDFSGYTDIALGSARLLGYRLT
                     VNFDRPYMSYSITDFWRRWHISLGSWFRDYLYIPLGGGRSHAGLNLFITMTIAGLWHG
                     AGWNFVIWGTFYGAMLAFERATGLSKTSTANGWKMLRILITLHLVLFGWILFRVDSME
                     TFRGVMGGLSRLEFRSPNLNWIQILVLCAGYGLHLVPVRWILRLRARYALLPVYAAAT
                     LCAIFLLIAGHLAVPQAVPFIYFQF"
     gene            complement(5351..6022)
                     /locus_tag="PLG78_09010"
     CDS             complement(5351..6022)
                     /locus_tag="PLG78_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23295.1"
                     /translation="MSCQTQTRAPEALPSILIVGDSIILGKLGEYLETELGRRGHEVH
                     RHAMVSSGLSGLHDYNWPEATYDYIDRYHPDLLIAMFGANDSLAVKNYADDAKFTFFS
                     DSGFRERYARKVRLFLSLNAPRVKTVYWVGQPNLDHHELTPKFPVLNEIYAQEVARVP
                     NGVFIPVWDFTSNAGKYKPVMADATGVVGPLKWDFVHFTPHGGKVLGDHIIEAIYNHR
                     RSHDR"
     gene            6164..>6517
                     /locus_tag="PLG78_09015"
     CDS             6164..>6517
                     /locus_tag="PLG78_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNE23296.1"
                     /translation="MDAESLIEEVRKEYSALDAKIADFVATTGLACPSGCGRCCENPN
                     VTATPLEAILLAASMPPNGTVPDSSICMFYESKGNGRGQCLAYEARPLVCRLFGFASH
                     RKRDGSIGFRPCSVHS"
BASE COUNT         1626 a         1777 c         1601 g         1513 t
ORIGIN      
        1 ttctattgtt ccagatgagg gatgcgcgaa cacccacgtt cttatactgg ccgccggctg
       61 cttccactct ggcctgaatt tcagcgtcca catccgcctt tctttcttcc catgttttct
      121 ctggctgaac aaagaacaca tgatacttct cttgcagtgc ctgaaacgct tgaagagaag
      181 gaacgggtcc tgaaatgctt ctgcccaata ttttttctac atgatccgga ctcacactcg
      241 gataaaaacc ttcatcccca acaaagaaga aaaacccctt cttgcccttg cgattgcaat
      301 ctagaatgga atgcttcgcg tagtagtacg cggcgagctc ataggattct tgcccggttc
      361 cgccaccgcc ttcttctatc cagaacttgc tgagcacttc gtcgagtcta ttgtccgcct
      421 cgaactgccc gacctgtaaa ggagcctgat ccgaattggc atctccaatg gcagcaaagc
      481 tgatcgcggc acctttcacg tagcccttca gttcaatctg tccaagaaat aaaggcagct
      541 tttcatagac cacttttgta tcattgccgc gtgagcgcgt aacgtccagc gcaaccacga
      601 tggcagtctc attcatgcat tcccgatttt tgccatgcac attcaagagt ggatgaactt
      661 ctcttctagc agacgcatcc gctgcgttga ctccatatcc ctggtaagag aagacgccac
      721 caccggagga tctcgcttct cttgcaatgt cctgactgta atagccgccg cccatacatt
      781 actcctgaat cttgaactcc aaaaatttat gaggaccaaa tacttcttct ctcagatcat
      841 ctgcctttgc atacatttgc caggcatcct gggcgcgctg gagcgcgctt gtccgagtaa
      901 agaatttgat gaatcgctgg atgcgttcat ccacgttatc cggcattgtg tctagctgca
      961 cgtttccacc caggagataa atcatgcatt tgccaataga atagagatcg gaagccggaa
     1021 gaggttgttt cttctccgcc acttctggag gagagaaatg atcattgatc gccttgaacc
     1081 cctgccctgt ttccgctggc ttgtgaaccg catacgccca atcgatgacg aacacattgt
     1141 ggtccggcgc gctgatgagt atatgagaag gatctatgtt tccatggatg atcccgcggc
     1201 tgtgagcgtg tcccacggca gcgagagttc ttcgcaggat ccagaggatg tgttgttgtg
     1261 gaatgccctg cggataactc tctcgcactg tgcgaagatc agatgctcga attctcgcaa
     1321 gcaccaggcc gatttcaccc gaagaagcgc gaaattggtc tagaagctcc ggcaaatgtt
     1381 tgttgtatac ggtgtctccc tggtgaagag cgcgaagggt gttcgcttca ttttgcagaa
     1441 gatcgttgtc acccgtgctc atagcgactt tcaaaatgac ctgacgatcg gatctgtttg
     1501 tcccgccgta gatcgaggcg atatcgcccc gggccacgta ttctgtaacc gaatagcgat
     1561 ccacaggcgt ttccacttca aaaggtgaaa acctctcgcg ggcggttctg gtttcgcgcg
     1621 atgcaagctc cctctgtgtc tcgctttcca cttttctgcg gccaaagatc ttccgccgca
     1681 gccagtgata catgtcatcc ccggcgcggg tgagaaaatg ttttaccagg aagattccaa
     1741 ccgcgcttgc aatgcccgca agcgcaatgg cccacggctg ggacgcaata gagccaatcg
     1801 cagacgcagc cgcgcccagg agttccagca tggatcagga cagggtcaac cgggaaaacg
     1861 gtcaaccctt tgcagtgtta ttttcgcttc gcgcttcttc ctgttttttt agacgcggat
     1921 cttttgcccg tgctctttct cgatcctgat ttgccagatt gagttttgcc agtcagacgt
     1981 gcggttcgcg catcgcgacc tttggtctct tctagatgaa tatgctccag atggtttgcc
     2041 atgtgcatgg cgtgcaggtg ggagaattcg tctttagaca ggcgatcgaa tgcgggatga
     2101 atggccatct ctccgtcatg cgctttgaac tgcacgatcg cctttcgcag acgtttcaat
     2161 gcctgcttcg catccccttc cgcacgcact ttgggtgcat ttggattggg cgacccgggt
     2221 ttcatgaacc cgttcttttg ctgggaacga aagatcattc taccgattgt cttgcgaagg
     2281 actgcgggga gtagtcctgg atacccgctc accgaatttt caaatgaatt tgcgaggtga
     2341 tcaaagattt gtgcttcaga ccagagcccc gtggctttgt aagccggtgc tttttcaatt
     2401 cgctccaggt gttcttccgc ctgggataaa gttctaaaac gaagttcgct catgatccat
     2461 tttccgcaga agctcccggg cgaacagtca aaaccgtgca cgcgggatcg cagaaggatg
     2521 gaggcagacg tcaggcgcct ttcaaaactg gaaatagatg aacgggactg cttgcggaac
     2581 ggccaggtgt ccggcgatca atagaaagat cgcgcataaa gtggcagctg cataaactgg
     2641 aaggagagca taccttgcgc gcagtcgcag gatccatctc accggaacca ggtgcaatcc
     2701 atatccagcg catagaacca ggatttgaat ccagttcaga ttgggagatc taaactctag
     2761 ccgcgaaagt cctcccatca ctcctctaaa ggtttccatg gaatccacgc gaaatagaat
     2821 ccagccaaac aagaccaggt gcagagtaat caagatgcgc agcattttcc aaccatttgc
     2881 ggtggacgtt tttgacagac ccgtcgcacg ctcaaacgca agcatcgcac catagaaagt
     2941 tccccatatt acgaagttcc agccggcgcc atgccacagc cccgcaatcg tcatggtgat
     3001 gaacaaattc aacccggcgt gagagcgacc acctcccagg ggaatgtaga gataatccct
     3061 aaaccagctc cccagagaaa tatgccatct tctccagaag tcagtgatgg aataagacat
     3121 gtaaggacgg tcaaaattta cagtcaggcg atatccaagt agcctggcac ttccgagcgc
     3181 aatgtcagta taaccagaaa aatcgccgta tatttgaagg ccatatccat aaactgccgc
     3241 cagtatttcc gcagatgtaa acatgccagg attctggtaa acgcggtcca ccatttgcac
     3301 agcgatccag tcagccgcga gtttcttcgt aagccctttc ataataagaa agatgcctgt
     3361 ctgaaactgt cgcatactcg gtgtaaggtg gcgcatgtct tctctcagac gcagaaggaa
     3421 atgcctcgcg cgcacgattg gcccggccac aagttgaggg aagaaactca caaagaacaa
     3481 tagtctgggc aggctcttct ccgccggctc cacaccgcga tacacgtcga tcgtataaga
     3541 aagactctga aatgtataaa aggaaattcc cacagggaga atcaggctca gaacggggaa
     3601 atccgcgtgc agtccaaacc atcctgcagc ctgcacggcg cttgacgcaa aaaagttata
     3661 gtacttaaag aaaaacagga ttcccagatt gctcgaaagg gaaaggaaca gtagaaatcc
     3721 ggcccatctt ctggacgatt ctgtatcttc cagacgcttg ttttcaatgc ctcgagcaag
     3781 gatgtagtcc acggtcgcgg ttagaacaag aagccccagc gcccagagtc gccaggatgc
     3841 atagaaatac atagaggcag ccataagcac atacatgcga aggcgaacga agcgtctgag
     3901 aagccccgcc atgagaatga ctccaccgag ataccacgca taacgaacgg aatgaaaaag
     3961 aatggttcga tcttctgatt ttttctccac tacagtgctg gctttctctg gcggactatc
     4021 tccggcaaaa atgtcgcgaa tcatcgcgtc tgccactgca ttgcctcccg acacagacag
     4081 atgcatataa tcagatagaa aaaaaccctt tgccatgaat tcagaaacgt gagaaggccc
     4141 gagagccacc gcagtatcat agaacgcgaa accaaactct cgcgcgatct tacgctgcac
     4201 ttctcgaacc tgtgtctgtg ccgcgacagg aaccagtcct ccgtccgctg attttactcg
     4261 atcgagcgga cctacaatga gcactgcggc atcaggcaat gcctttcgaa gcctcgtgta
     4321 gtatgcgcgc aggtcagttt cgtagacgga ctgcgaaaaa ccattgtagt acgtgtcaat
     4381 ggaagcagac tcattgaggc caaattgaaa aatcacgaga tcaggagcgg cggctctcca
     4441 tccgcagatg aagttttcat ccgataccgt gcgaacccag gcctgatgga tgcccatggt
     4501 cactagagct gaataagaaa ctccggacga agactcgaag atagccgcgt ggatcacagg
     4561 caagccagac gcctgcattg tgattgcatg aacaccttct gcgcctgtct ctgctaccgt
     4621 caggcgacat gcaccttcta ccacctttgt cgctgtatcc agagtttttc cgttgcttgc
     4681 aaggttgacc gtaccgtttc cagaccagac cactctggcc ttgtgaatcg gacttgaacc
     4741 atccggaaat gaaaactgca cgttcgcagg atctgcgatc gactgcgaag atctgctgaa
     4801 aaatccaagt ttagaatcca ctccctgagg aaacgctcct gaaatttttt ggagcgaaaa
     4861 tccagtgggt tcaatgcgaa tgtcctgcac cactgaatcc ggtgaattta caatgcttac
     4921 aattccgcgg ccgccatctc cgtatctgga ttgaagatga cgacggagcc gcgaagatac
     4981 gttgtctgcc cacatcagtg aatcgcccga gtgaacgatt cgcacgggcc tatcctttct
     5041 tccaagcgtg cgaaggacac cctccagtgc cttttcattc tcgagaagca ccttctcttt
     5101 ctgcggaact ccggtctttg tctcgcctcc ggtcttcaga tcacgcgaag tggcttcgcc
     5161 cggggccgtg cgatgatcgg aaggtgaatt tttcgcgccc ggccatggaa agccgggaag
     5221 gtcaggggca agatcattga tgcgcggcag ccaggacctg acctctgccg gtacttctgg
     5281 cctctctgcc agcagcgccg ccagagtcgt aaccccggcc acgataaaaa caaaagcagc
     5341 ctttctgaat tcaacgatca tgagaccgcc gatggttgta gatcgcctct ataatatgat
     5401 ccccgagcac cttaccgccg tgaggtgtaa agtggacaaa gtcccatttg agtggaccga
     5461 cgaccccggt tgcatccgcc atcacaggtt tgtattttcc cgcgttcgaa gtaaagtccc
     5521 atactggaat gaacacacca ttgggaactc gcgccacttc ttgcgcatag atttcattga
     5581 gcacagggaa tttaggagtc aactcgtggt gatccaggtt tggctgtccc acccagtaga
     5641 cagttttcac tctcggagca ttcagactga ggaaaagtcg caccttgcgc gcataacgtt
     5701 cgcgaaatcc cgaatcacta aagaaagtaa actttgcatc gtccgcgtag ttcttcacag
     5761 cgaggctgtc atttgcacca aacatggcaa tcagtagatc cggatggtat cggtctatgt
     5821 aatcatacgt tgcttccggc cagttgtaat catgtaatcc gctgagtccg gaagacacca
     5881 tagcatgacg atgaacctca tgtccgcgcc ggccaagctc cgtctccaga tattcgccaa
     5941 gcttccccag aatgatggaa tcccccacaa tcaaaatcga cgggagtgct tccggggctc
     6001 tggtctgtgt ctggcaagac accagaatgc caaaaaaaac aagtaagatt gagggcaagg
     6061 cccgcattgg acgaaattca aacgggcacg tatccgcgaa agcaggaatc ggcaatcgct
     6121 atggttccct tgacattttg aatacccgct ccacccctgg ggcatggacg cagagagcct
     6181 gatcgaagag gttcgaaaag aatactcggc gctggacgct aaaattgcag attttgtggc
     6241 cacgaccggg ctcgcctgcc cctccggatg cggacgatgc tgcgaaaacc cgaacgtcac
     6301 ggcaacacct ctcgaggcca tcttgcttgc cgcttctatg ccgcctaacg gcactgtacc
     6361 ggactcttct atatgcatgt tctatgaatc gaaaggaaat ggccggggac aatgtctcgc
     6421 ctacgaggca aggcccctcg tgtgccggct gtttggcttt gcgtcgcaca gaaagagaga
     6481 cggaagcatt ggtttcaggc cttgcagcgt gcacagt
//
LOCUS       DAOQJL010000170         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6631_length_6512_cov_4.513861, whole genome shotgun sequence.
ACCESSION   DAOQJL010000170 DAOQJL010000000
VERSION     DAOQJL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_6512_cov_4.513861"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PLG78_09020"
     CDS             <1..1599
                     /locus_tag="PLG78_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23297.1"
                     /translation="ISGPRRAYLDLSRRGRCLNISLDLPRVFPDIRRYLTRMPQLEKL
                     SLKKPDAMKGVRVLLIHHITGEILATIRAIENMNAEFLRVLFVKYAGIVPPEYLETLL
                     SLPENQFAFHGLNKIEASGSVEGFYILSRQYSPITRFQDLDRRLAEKKLAFFEAMQLA
                     AGNLFFREAGICIRTNKKLLLVEDGGYLAPQLNALSLEKKSVRDAMIYYGFDPGKKNE
                     GLKEILPVSDKDLEKPLGEWLSAFFTGSVEHTRNGYDRLVGVQKKYNRLQFPALTIAV
                     SKTKREMESREVSVSILHAVESILHGQGLVLSNRRALVLGSRGAIGQCLVQHLKSRIQ
                     PDRVAGVDLVINSKNSSAQGITEAAALEKLGKEHVRDADIILGVIGKSIFTDEWIEDL
                     ILNGKRPHVFFASGSTKTLEFTHLSHYVEKLRQAQSPRIRGQAARVDAEPIRDPQTGL
                     IQGTQVRIELDKKTRTLHLLGGLTPINFLYYGVPTETMDPVLTQLIQSAAGLLARQKS
                     GKGLPPRVLAVDHEITVDAELIKK"
     gene            1596..4571
                     /locus_tag="PLG78_09025"
     CDS             1596..4571
                     /locus_tag="PLG78_09025"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNE23298.1"
                     /translation="MNPEEISARIQRIENDLAELKAAVRQQSSSSSTQTAETRPTSSV
                     TTPPRPPAKPGIVTETLSRFKEDNVEVFLGGNLLGKLGIVAIVLAVSWFIKLAFDNQW
                     VNESGRIYTGLLVGFAIAGVAVSLAKRNMRVVPAPLFGAGISIVYISLFSAYHFYYLL
                     GIQEVFVSLFVLSVAATVLSLTANRQSLYVFGLIGSFLAPIMLSRGENSYRFLFTYIA
                     VIQLGFLWVSWYRPWRISAVIVLAGTAIMWTGWASNYLNKSGFVFPMAILVFTLAAFF
                     VRHTFLSLRHKTAPSIPDLIILPLSSLFYASAGYYLTRHHYPNAISHFLLVLTLLMAF
                     FYIRYTAIEARDNSTRSILFFQILVFTFASISQFFDGRLLTLSWIVFASALSISSVTL
                     KTRPVLLVSMIAWILAVFRLIFAEYTPSPFHPLVNVRFALYLVAGVGASITYLRHRKD
                     PFETLVRGFAFLSMFLFVFGTLLENRDFIASPHYRNLGYSYVLGLYAAIFLVYGFLRN
                     HKTMRLCGVFLAALVVMKLYFYDIWTMSRLVRIIAGFSLGVALVVLSIVYQKYRDKIL
                     QIGKTLMMWLAAPLALFILLSSNNLQAETIRAGSFKKVASLKPDNPSALKDSKTAYGR
                     FTMNDDLVRAGSSSVRIAWKDRALPYVLQRVREDKQRTGAAVPEVIFEEGQRNARVFV
                     LRLPEAPGGTVWTDLEVESTEMYESGVQVEAGEKPGDWNVIGHRNIYNYAGASDRVIS
                     IDAGRSKYLRVTLDKNLHMVFPKVHYGPSRDREVSFDIPPEEWKITQNTDEHATEFLF
                     NNKDQRKIERLTLQFAEPRFHRRVEVYVMQRASHEFVINTTTGFMKRTNDPADQVLDL
                     ASPATDAVKVVVFNEDDAALKLTKSTAFAPLEQVIFQLPSADELATEEGNPEIKIYAG
                     NEYVGFPDYDLSRTMDDSVVSASFTPTALGDNAAFAYSAVEPPLSSWIIRIAFYIGLA
                     ALAIPGLRVFRDYAQSVKS"
     gene            4597..5382
                     /locus_tag="PLG78_09030"
     CDS             4597..5382
                     /locus_tag="PLG78_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23299.1"
                     /translation="MKSATLVLIVTLSMGGQLLAQTETCSRGDCKNGPGTLQLPNGSR
                     YEGEFKNGNFHGQGVYTLPSGVYTGEFKDNKIEGKGVFLLKTGRKLEGNFVNGKLNGE
                     GTETLPDGTRTQGQFESNKFVKGTLTRPDRTTFTGSFTDGQLQGKGESTLPDGSNYTG
                     EFVNGVFQGKGTLTHSDGGVYTGEFVNGKKEGKGIYKYANGDQYTGDFKNNFKEGTGV
                     YSYADKRKYEGQFQNDQPHGHGTYTAQDGKSRTGTWVRGQEQK"
     gene            complement(5386..6414)
                     /locus_tag="PLG78_09035"
     CDS             complement(5386..6414)
                     /locus_tag="PLG78_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE23300.1"
                     /translation="MRTAAEQQATYRLRLRASVVSIVVGFLVCLLKFAGFLTTGSYAI
                     LSDALESIINVAAASFAAYCIVQGARKADFDHPYGRGRLEYFSASLEGALILLAGVII
                     LNESIPKLFAGNVVGRMDLGLMFTGVGTVANGILAAFLIRSGKKHHSMALEADGHHVW
                     SDVVSSIGLFVGLVLVAISKLPWLDPLLACIMALWILWSGFRILRQSFFQLMDRASPD
                     VVTKVTSAIAELRKPEMIHPHRMRLRESGPYVLVDFHMIVPRFFTVRQLHDLEEHIHR
                     NLESILDRPVDLMLHNDPCTSNNCGYCSMKNCRVRKKKQTSQLKLDPQHLLEDISHPV
                     HTRDRRGD"
BASE COUNT         1747 a         1684 c         1546 g         1535 t
ORIGIN      
        1 atttcgggcc cgagacgcgc ctatctcgac ctttcaagaa gaggcagatg cctcaacatt
       61 agcctcgatc ttccgcgcgt attccccgac attcgccgct atctcacacg gatgcctcag
      121 ctggaaaagc tcagcctgaa gaaaccagac gcaatgaaag gcgttcgcgt actcttgatt
      181 catcatatca ccggagaaat cctggccaca attcgcgcga tcgaaaacat gaacgcggag
      241 tttctgcgcg tactctttgt taagtacgcg ggtattgttc caccggaata tttagaaact
      301 ttactatctc ttcctgaaaa ccagttcgcc ttccatgggc tcaacaaaat tgaagcttcc
      361 gggagcgtgg aagggtttta cattctatcc agacaatatt cgcccattac gcgattccag
      421 gatctggatc gcagactggc ggaaaagaag ctcgctttct ttgaggcgat gcaactggca
      481 gcgggcaatc tgttttttcg cgaagccggt atttgcattc gaaccaataa gaagcttctg
      541 ctcgttgaag acggcggtta cctggcgccg caattgaatg ctctatctct ggaaaaaaaa
      601 tccgtacgcg acgcaatgat ctactacgga tttgatcctg gaaagaagaa tgaaggtcta
      661 aaagaaatcc tgcctgtctc tgacaaagac ctggaaaaac ccctgggaga atggctcagc
      721 gctttcttta ccggcagcgt agagcacaca cgcaatggat acgaccgact ggttggagtt
      781 cagaagaaat acaatcgcct tcagtttcca gcactgacca ttgcagtttc aaaaacgaaa
      841 cgagaaatgg aatcgcgcga agtttcggtt tctatcctgc acgcagtgga aagcatttta
      901 cacggacagg ggcttgtcct ttctaatcgc cgggctctcg tgcttggaag cagaggggcc
      961 attggtcagt gtctcgtgca acatttgaaa tcgcgcattc aaccggaccg cgtcgccgga
     1021 gtcgatctgg taatcaatag caagaattca tccgctcaag gaatcacgga agcggccgca
     1081 cttgagaaac ttggaaagga acacgtgcga gacgcggaca tcattctggg tgtgattgga
     1141 aaatccatct tcacggatga gtggatcgaa gacctcatcc tgaatggaaa gcgtccccat
     1201 gtgttttttg caagcgggtc cacgaagact ctggaattca cgcatttgtc gcattacgtt
     1261 gaaaaactca gacaagctca atctccgcga attcgtggac aggcagccag agtagacgca
     1321 gaaccaatac gagaccccca gacagggctc attcagggca cgcaggttcg catagaactg
     1381 gataagaaaa ctcgaaccct ccatctactc ggtggtctaa cacccattaa ctttttgtac
     1441 tatggggtgc cgacagagac catggatcca gttctaacgc agctgatcca gtctgcggca
     1501 ggcttgctgg cacgacagaa gtccggcaaa ggtttaccgc cgcgcgtact tgcggtggat
     1561 catgaaatta cagtggatgc ggagctgatc aagaaatgaa tccagaagaa ataagcgcgc
     1621 gcattcaaag aatcgaaaat gatctcgcag aactcaaagc agcagtcaga caacaatcgt
     1681 catcctcatc tacacagacc gcagaaaccc ggccaacttc tagcgtaacg actcccccgc
     1741 gcccgcccgc aaagccagga attgttacag aaactctatc acgcttcaag gaagataacg
     1801 tcgaagtatt ccttggcgga aacctgcttg gcaaactcgg aattgttgct attgttctgg
     1861 ccgtttcgtg gttcatcaaa cttgcattcg acaaccagtg ggtcaatgag tcgggtagaa
     1921 tttacaccgg cttactcgta ggtttcgcaa ttgcaggtgt tgcagtgtct ctggccaaaa
     1981 gaaacatgag ggtggtacct gctcctctgt ttggggctgg catttccatt gtctacattt
     2041 cgctattcag cgcgtatcat ttctactacc tgcttggcat tcaggaagtt ttcgtaagtc
     2101 tgtttgtgct gagcgtcgcc gcaactgtgc tttctctcac tgcaaataga cagagtctct
     2161 acgtcttcgg gttgatcggt tcatttctgg cacccatcat gctttcgcgc ggggaaaact
     2221 cctaccgctt tctctttaca tacatcgcag tcattcaact tggttttctg tgggtgagct
     2281 ggtacagacc ctggcgcatt tctgcagtca tcgtgctggc aggaactgcg atcatgtgga
     2341 ctggatgggc ctctaattat ctaaacaaga gtggctttgt ttttccaatg gccatactcg
     2401 tattcactct tgccgctttc ttcgttcgac atacgtttct atctctgcga cacaagacag
     2461 ctccatccat tccagatttg atcattctgc cgctatcctc tttgttttat gcctccgctg
     2521 gttactatct gacgcgacat cattacccga atgcgatcag ccactttctt ctggttctca
     2581 ctctgctcat ggcattcttc tacattcgct acacagcaat tgaagccaga gacaacagca
     2641 ctcgcagtat tctcttcttc caaatactcg tattcacgtt tgcatccatt tcgcaattct
     2701 ttgatggcag gcttctgacc ctttcctgga tcgtatttgc ttccgctctt tccatttctt
     2761 cggtcactct gaaaaccaga cccgttctcc tggtaagcat gattgcctgg attctggctg
     2821 tattccgcct catctttgca gaatacactc cctcgccgtt tcatccgctg gtcaatgtgc
     2881 gattcgctct ctacctggtc gcaggcgtag gcgcctccat cacgtatctc aggcacagaa
     2941 aagatccgtt tgaaaccctc gtgcgcggat ttgcatttct ctccatgttc ttgttcgttt
     3001 ttggaacttt gctagaaaac agggatttca tcgcgtcgcc gcactatcgc aacctggggt
     3061 attcgtatgt tctgggacta tatgccgcga tcttcctcgt ctacggtttc ctgcgaaatc
     3121 acaaaaccat gcgactgtgt ggcgtgtttc ttgccgctct agttgtaatg aagctctact
     3181 tctatgacat ctggaccatg agtcgccttg ttcgcatcat cgcagggttc agcctgggcg
     3241 ttgcacttgt tgtactcagc atcgtgtacc aaaagtatcg cgacaaaatt ctacaaatcg
     3301 ggaagactct tatgatgtgg ttggctgctc ctcttgctct attcatacta ctttcgtcta
     3361 acaacctgca ggcggaaacg atccgtgctg gtagcttcaa gaaagttgcc tctttaaagc
     3421 cggacaatcc atctgcgcta aaggattcaa aaacggccta cggaagattc acgatgaacg
     3481 acgatctggt tcgcgcagga tcctcctccg tccgcattgc ctggaaagat cgcgcgctcc
     3541 cgtacgtgtt acagagagtg cgcgaagaca aacaacgcac cggcgcggca gtacccgaag
     3601 taatctttga agaaggacag agaaacgctc gagtctttgt cctccgattg cccgaagcac
     3661 cgggaggaac cgtctggacg gatctggaag tagaatcaac cgaaatgtat gagtcgggcg
     3721 tccaggtaga agcgggagaa aaaccaggag actggaacgt gatcggtcac agaaacatct
     3781 acaactacgc gggcgcgtcg gacagagtca ttagcattga tgcaggccgc tccaaatatt
     3841 tgcgagtgac actcgacaag aaccttcata tggttttccc aaaagtgcac tacggccctt
     3901 cgcgcgatcg cgaagtctct tttgatatac caccagaaga atggaaaatc acgcagaaca
     3961 cagacgaaca cgctaccgaa tttctcttta acaacaagga ccagcgcaag attgagagac
     4021 tgacgcttca gtttgctgaa ccacgctttc acaggagagt ggaagtgtac gtgatgcagc
     4081 gggcttcaca tgaattcgtg atcaatacga ccactggatt catgaaaagg accaacgatc
     4141 ccgcagacca ggtactcgat ctggcaagtc ccgcaacaga tgcagtgaag gttgttgtat
     4201 tcaacgaaga cgacgcagca cttaagctaa caaaatccac agcgttcgct ccgctagaac
     4261 aggtgatatt ccagcttcct tctgcagatg aactggcaac ggaagagggg aatccagaaa
     4321 tcaagatcta tgcaggaaat gaatacgttg gattcccgga ctacgatctc tcacgcacaa
     4381 tggacgattc agttgtgtcc gcaagtttca cgccgacagc tctcggtgac aacgcagcgt
     4441 tcgcatactc agcagtggag ccaccgcttt ccagctggat catccgaatc gcattctaca
     4501 ttggtctggc agcactcgcc attccaggct tgagagtgtt ccgcgactat gctcaatcag
     4561 taaaatcata aagaacttaa acttttgagg atcgccatga aatctgccac tttggttctg
     4621 atcgtcacac tttccatggg aggccaactt ctcgcccaga cagaaacatg ctcgcgaggg
     4681 gattgcaaaa atggtccggg aactctgcag cttcccaatg gctcgcgata cgaaggcgaa
     4741 ttcaagaatg gcaatttcca cggccagggt gtttacactc tgccgtccgg cgtatatact
     4801 ggagaattca aggacaacaa gatcgaaggc aaaggcgtct ttttgctgaa aaccggcaga
     4861 aagcttgagg gcaacttcgt aaatggaaaa ctaaatggag agggaacaga aaccctgcca
     4921 gatggaacca gaacgcaggg ccaatttgaa agcaataagt ttgtgaaggg aactctgacc
     4981 aggccagacc gcactacatt tacaggatca ttcacagatg gacagcttca gggcaaaggg
     5041 gaaagcactc tgcctgacgg aagcaattac accggggaat ttgtgaacgg agtttttcaa
     5101 gggaaaggga ctctgactca cagcgacggt ggcgtctata ctggcgaatt cgtaaacggg
     5161 aagaaggaag gaaaaggcat ttataagtac gcgaacgggg accagtacac gggagatttc
     5221 aaaaacaatt tcaaagaagg caccggcgtc tactcgtacg cggacaaacg caaatacgaa
     5281 ggccaatttc aaaacgacca accgcacgga cacggaactt acacggcgca ggatgggaag
     5341 tctcgcacag gaacgtgggt ccgaggccag gaacagaaat agtacctagt cccccctccg
     5401 atcacgcgtg tgtactggat gcgaaatatc ctccagcaaa tgttggggat ccagtttaag
     5461 ttgtgaagtt tgcttcttct tgcgaaccct gcaattcttc atcgaacaat aaccacagtt
     5521 attcgaagtg catggatcat tgtggagcat cagatccaca ggtctgtcaa gaatgctctc
     5581 cagattccga tgaatgtgtt cttccagatc gtgcaattgc ctcactgtga aaaagcgcgg
     5641 cacaatcatg tggaaatcta ccagaacata cgggcctgat tcgcgtaacc gcatgcgatg
     5701 cggatgaatc atttccggct ttcgcaattc cgcaatcgcg ctcgtcactt tcgtaaccac
     5761 atccggactg gcacgatcca ttagctgaaa gaaactctga cgaagaattc gaaacccgga
     5821 ccacaaaatc catagtgcca tgatacacgc aagcaatgga tccaaccacg gcagttttga
     5881 aatggccacc agaaccaacc caacaaacaa accaatactt gaaacaacgt cagaccatac
     5941 gtgatggccg tccgcttcta gagccatgct gtgatgtttc ttgccagaac gaatcaagaa
     6001 agcggccagg attccgttcg cgacggtgcc cactcccgtg aacattagac ccagatccat
     6061 cctgcccact acattaccgg caaacaactt tggaatagac tcattcaaaa tgataacgcc
     6121 agcgagcaaa atgagagcac cctcaaggct tgctgaaaaa tattccaggc gaccgcgacc
     6181 gtatggatgg tcaaagtccg cctttcttgc accctgcaca atgcagtatg cggcgaaact
     6241 ggctgcagcc acatttatga tactttcgag tgcatccgaa aggatcgcgt aggaacctgt
     6301 agtgagaaac cctgcaaact tcaacaagca tacaagaaag ccaaccacga tggacacaac
     6361 ggaagcccgt agacgcagac gataagttgc ctgttgctct gctgcagtgc gcactggttc
     6421 acagaatcga tctttccgag gatggcaacg aaaaagatcg aaggaacgcg ctacgaaccg
     6481 aaaaccagca ctagttgaga ttcaatttca ca
//
LOCUS       DAOQJL010000171         6470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6693_length_6470_cov_4.605924, whole genome shotgun sequence.
ACCESSION   DAOQJL010000171 DAOQJL010000000
VERSION     DAOQJL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_6470_cov_4.605924"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..639)
                     /locus_tag="PLG78_09040"
     CDS             complement(61..639)
                     /locus_tag="PLG78_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE23301.1"
                     /translation="MRKFILILCVLSLFAQCKKEIHPITSIPDDSLYQLDSEWKTQKN
                     TVIHLRDLSGRVLIVGLIFTRCTAICPTIVADMKTIEKNIGWFHKDDVGFLLVSIDPT
                     RDTPEAMRSYMKKMNLDESRWTFLQAKPPDVADLASLLGVKISPLPDGSFSHSRILTV
                     LSPSGQIKFQDPRVSEDGANFAEAARQAADFH"
     gene            complement(636..1385)
                     /locus_tag="PLG78_09045"
     CDS             complement(636..1385)
                     /locus_tag="PLG78_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNE23302.1"
                     /translation="MSAARLTVLSLALLAFELSAATPDGMSFVPPGVFQPFFKNIAPQ
                     KVRGFYLDQTQVTNAEYLIFLKANPSWEKGSAKRIFADAGYLRGWTSPGSFPAESAEA
                     PVTDISQFAARAYCTWRGKRLPTLAEWEYAAGADPSHPMTDAENARLLAWYERNSTGR
                     PGRVRNVSNLHGIYDMHGLVWEWVDDFNSVMISDESREGGSDKQMFCGGGAANAADFR
                     NYAAFMRYAFRSSLRSNFTSSSLGFRCARNQ"
     gene            complement(1385..2782)
                     /gene="nirK"
                     /locus_tag="PLG78_09050"
     CDS             complement(1385..2782)
                     /gene="nirK"
                     /locus_tag="PLG78_09050"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNE23303.1"
                     /translation="MHTSTRSLLLLASIALTFGLTQCAKKTETAVLTTAPNVPPPIQR
                     STAAHVIVNLETREVTGTLSDGVQYTFWTFGGTVPGPMIRVREGDDVEFHLSNHPTSK
                     MPHNIDLHAVTGPGGGAASSFTAPGHSSQFSFKAMNPGLYVYHCATAPVGMHIANGMY
                     GLILVEPSGGLPSVDKEFYIMQSEFYTKGKFGEGGLQAFDMDKAVHEQPEYVVFNGSV
                     GAIAGDKALKAKVGDRVRIYIGNGGPNLISSFHVIGEIFDKVYQEGGVKVSQEQVQTT
                     LVPAGGSAMTEFRVDVPGTFILVDHSIFRTFNKGSLGMLKVEGPEDKNIYSGKQSDTV
                     YLPEGGAIQSIKEAQEAQKPLSRDESIAAGKIVYTQVCMACHQANGEGMPNAFPPLAK
                     SDFLMKDKQRAIRIVMNGLQGEVVVNGNKYNSVMPALGLKDADIANALNYVRNTWGNS
                     APDTITPAEVKALRK"
     gene            complement(2959..3816)
                     /locus_tag="PLG78_09055"
     CDS             complement(2959..3816)
                     /locus_tag="PLG78_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23304.1"
                     /translation="MKRISILLIAIAGFTTQLFAEDPAPTPAADNDSVLPIQEQNLGF
                     DPTRLQTMGTGSFEYRNLPSKAQDFTLRAGGDYRFSFLDDNRRWWGVSGSVPITMHQG
                     GKWGANKTGLGDLNLGVRRVFPGDIRQVGFVDVTWMLGGQPGLSGGHSLIAPGYGLSM
                     NIADRMQFAGTVTYVTDIGSKDWSKHEKIRVLHFKPMILYGLSDVLLLKGAFDVGYSL
                     TDGVETVPYFRKVNNKLQLTPSVTAGYLLGDDHRWMAFGTFQMPLDKFQIGYREQYSL
                     KVGANYFFD"
     gene            complement(3827..4297)
                     /locus_tag="PLG78_09060"
     CDS             complement(3827..4297)
                     /locus_tag="PLG78_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE23305.1"
                     /translation="MSKWSVFSVLIFAACLSGQSTQKTNEAYVQDLKSGDPALQREAC
                     KQLGLRRADNTVEAITALLTSSTDVDVQVSAAAALGEIAEVRSTSPLLKVLNTNPSPR
                     LQYASLAALGGIARINGTGKHKAAVIEAATRVKSGSTDSLLQDLADKIIQMYQK"
     gene            complement(4359..5012)
                     /locus_tag="PLG78_09065"
     CDS             complement(4359..5012)
                     /locus_tag="PLG78_09065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE23306.1"
                     /translation="MQVENNPADKHIARAKEKPLGWFEDLYRDAARNADLIPWADDEA
                     NPNLRSWAKTASGQEVLGSAKTALVVGCGLGDDAEFISRFVPEVTAFDISETAIAWAK
                     ERFANTKVKYMTANLFELATSCDFVFESYTIQALPLDMRSRAVARLPEFMNPGGRLLL
                     VARGRENEQPLADHPPWPLSRADLSPLDAVLQTETFEDYVEGTDPGIRRFRILYKKP"
     gene            5044..5550
                     /locus_tag="PLG78_09070"
     CDS             5044..5550
                     /locus_tag="PLG78_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNE23307.1"
                     /translation="MRNRICILFCSFTFLILQHCSSPQKSEPLPYEWMILTSTKDLPP
                     FAGAEILVRHTSSGSIGWVLNGERTTIHKLGLNGLGLPPDLVVRMGGPVETGKISFLY
                     RDPGTDAVHFSRYLPTLIEILKSEPANVVVFAGYSGWAEDQLASEIRRGLWVRSSKPR
                     RSIFVPET"
     gene            complement(5547..6149)
                     /locus_tag="PLG78_09075"
     CDS             complement(5547..6149)
                     /locus_tag="PLG78_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23308.1"
                     /translation="MKIETVKGKGMVVAGNDVDTDRILPARFMKSIVFSGLEEHAFED
                     ERKAFSRRGDLHPFDDPKNSDAVFLIAESNFGCGSSREHAVQSLARRGIKAIAAESFG
                     EIFLGNCRTVGVTAFTMSAADLAEARKLAKAGESFTIDLIQSRVSAGSQTFAMQMDEG
                     VRKQFTTGKWDTLAELLNAKDAAREKGMSLPNFGTEHGLF"
     gene            complement(6146..>6470)
                     /locus_tag="PLG78_09080"
     CDS             complement(6146..>6470)
                     /locus_tag="PLG78_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNE23309.1"
                     /translation="TTKAAHVKAVVVPGSHNVYNEAVALGLDRVFTDAGFEFRQPGCS
                     MCLAMNSDKLEAGQVCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALRGYVCDVREF
                     TGGVK"
BASE COUNT         1505 a         1637 c         1611 g         1717 t
ORIGIN      
        1 atactatcgc aagggaaatc ccatccggtt tatcatcacc ccgaacggga agattggggc
       61 tcaatgaaaa tcggcggctt gcctggccgc ttccgcgaaa ttggcgccat cttcgcttac
      121 gcgaggatcc tggaacttga tctgtccgga tggactgagt acagtaagaa tgcgcgagtg
      181 agagaagctt ccatccggaa gcggggaaat tttcacaccc agcagggaag ccaggtcggc
      241 aacgtcaggc ggctttgcct ggagaaaggt ccaccgtgat tcatctagat tcattttctt
      301 catgtaactg cgcatcgctt ctggagtatc acgggttgga tcaatggaaa cgagcagaaa
      361 tcccacgtca tctttgtgaa accatccaat gttcttttcg attgttttca tgtccgcgac
      421 gatggtcggg caaatggcag tacaccgtgt gaagatcaat ccgacaatca agactcttcc
      481 ggaaagatcg cgaaggtgaa tgactgtgtt cttttgtgtt ttccattctg aatcaagctg
      541 atacagagaa tcatccggga tagatgtgat cggatgtatt tcctttttgc attgagcaaa
      601 gagcgaaaga acgcaaagaa ttagaatgaa tttcctcact ggttcctcgc acagcgaaag
      661 cccagagagg aagaagtaaa attggaacgt aagctggatc tgaaagcata tcgcatgaaa
      721 gcggcgtagt tgcgaaaatc agccgcgttg gccgctccgc ctccgcaaaa catctgtttg
      781 tcggaaccgc cttcgcgtga ctcatcggag atcatgactg aattgaaatc gtccacccat
      841 tcccagacga gtccatgcat gtcatagatg ccgtgcaaat tggaaacatt ccgtactcgg
      901 ccaggtctgc ccgttgaatt tcgttcgtac caggcaagca gtctcgcgtt ctctgcatcc
      961 gtcattgggt gtgaaggatc cgctccagcg gcgtattccc attcagcaag agtagggaga
     1021 cgttttcccc gccaggtaca gtaagcccgt gctgcgaatt gcgaaatgtc cgtgaccggg
     1081 gcttctgctg attcagcagg aaaagatccg ggcgatgtcc atccgcgaag gtaacctgca
     1141 tctgcgaaga ttcgttttgc ggatcctttt tcccacgatg gatttgcttt caagaagatc
     1201 aggtattctg cgtttgtaac ctgagtctgg tcgaggtaga agccgcgcac tttttgcggt
     1261 gcaatgtttt tgaagaaagg ctgaaaaacg ccaggcggaa caaaagacat tccgtctggc
     1321 gtcgcagctg acagttcaaa ggcgagcaag gccagtgaaa gaacagtcag ccgcgcagca
     1381 ctcattattt ccgcaacgcc ttcacttctg caggcgtaat cgtatcaggg gcagaattgc
     1441 cccaggtgtt tcgaacgtag ttgagcgcat tggcaatgtc tgcatctttc agtccgagtg
     1501 caggcattac actgttgtat ttgtttccat ttacaaccac ttcaccctgc agaccattca
     1561 tgacgatgcg aatggcgcgt tgcttgtcct tcatgagaaa gtctgatttt gccagaggtg
     1621 gaaatgcatt tggcatgcct tctccatttg cctggtggca ggccatgcat acttgcgtgt
     1681 atacgatttt tcccgcagcg atgctttcat ctctcgaaag aggtttttgc gcttcttggg
     1741 cttctttgat ggattggatg gctccacctt cggggaggta gacggtgtct gactgttttc
     1801 cggagtaaat atttttatct tccgggcctt ctactttcaa cattcccagg gatcctttgt
     1861 tgaatgtgcg aaagatggaa tgatcaacca gaatgaatgt acccggaaca tctactctga
     1921 attcagtcat ggcagatccg cccgcaggaa ccagagtagt ctggacttgc tcctgactta
     1981 ctttgactcc accttcttga tatactttgt caaagatttc tccaatcacg tgaaaggatg
     2041 agatgagatt cgggccgccg tttccaatgt agatgcggac tctatctccc actttggcct
     2101 tgagtgcttt gtctcctgcg attgctccga cggaaccgtt gaacacaaca tactcaggct
     2161 gttcatgaac tgctttgtcc atgtcaaacg cctgtagtcc tccttcgcca aatttcccct
     2221 tcgtgtagaa ttcactttgc ataatgtaga attccttatc aacagaagga aggccgcctg
     2281 acggttctac aaggatcagt ccatacatac cgttggcaat gtgcataccc accggggcgg
     2341 tggcgcaatg atatacgtag agacctggat tcattgcttt aaatgaaaac tgagaagagt
     2401 gaccaggtgc cgtgaacgac gaagcggctc cgccacccgg gccagtcact gcatgcaagt
     2461 cgatgttgtg aggcatctta ctggttgggt gattgctgag gtgaaactct acgtcgtctc
     2521 cttcgcgtac gcgaatcatt ggtccgggta ccgtgcctcc gaatgtccag aaagtatact
     2581 gcacaccgtc ggaaagcgtt cctgttacct cacgtgtctc caggttcaca atgacgtgtg
     2641 cggccgtaga tctctggata ggtggaggaa cgttgggagc cgtggtcaat acggcagttt
     2701 ctgtcttctt tgcgcattga gtcagtccga aagtgagggc aatggacgcc agaagaagga
     2761 gagatcgagt acttgtgtgc atggagcgcc tccgggatgg cactatccta tcatgttcgg
     2821 agtctgagtg ccttgacgcc cgtcaattcc ggctacgttc ccggatatga catttgtcat
     2881 ttgggtctgg aaggtttaga aagttgaatg aattgccgcg ctcttgaaaa gaacgcggcc
     2941 gagaggctaa cgattgagtt agtcgaagaa gtagttggca cccactttta gagagtattg
     3001 ttctctgtag ccaatctgga atttgtctag aggcatctgg aatgttccga acgccatcca
     3061 gcgatgatca tccccaagta aatagcctgc agtgacagac ggtgtgagct gaagcttatt
     3121 gtttactttt ctaaagtatg gaacagtttc cactccatcc gtgagagagt aaccaacgtc
     3181 aaaagctccc ttgagtaaga gtacgtcaga caggccgtag aggatcattg gcttgaaatg
     3241 aagaactcga atcttttcat gcttggacca gtctttggat ccaatgtctg tcacgtatgt
     3301 tacagtgccc gcgaattgca ttctgtctgc gatgttcatg gaaaggccgt aacctggagc
     3361 aatgagactg tgtccgccag atagacctgg ttgcccgcca agcatccacg tcacatccac
     3421 aaaaccaacc tggcgaatgt ctccaggaaa tacgcgacga actccgaggt tgagatctcc
     3481 cagaccagtt ttattcgcgc cccattttcc accctggtgc attgtgatcg gcactgagcc
     3541 ggacacaccc caccagcggc gattgtcatc caggaagctg aagcgataat ctccaccagc
     3601 gcgaagagtg aagtcctgcg ctttgcttgg aagatttctg tattcgaaag agcctgttcc
     3661 catagtttgc aggcgagtag gatcgaatcc cagattctgt tcctgaattg gaagaacaga
     3721 atcattgtct gctgcaggtg tgggtgccgg atcttctgcg aagagctgag ttgtaaaacc
     3781 agcgattgca ataaggagga ttgaaattct tttcacgcga tgacctctac ttttgataca
     3841 tctggatgat cttgtctgca agatcctgga gaagagaatc cgtagatcct gacttcaccc
     3901 tggtggcagc ttcaatgact gctgctttat gttttccagt accattgata cgtgcgatcc
     3961 cgccaagcgc ggccagagaa gcatactgca gacgcggcga cggattggtg ttcaacactt
     4021 tcagaagagg gctggttgaa cgaacttctg caatttctcc aagtgcggcg gcagcactca
     4081 cctgcacgtc cacatctgtg gaagatgtaa gaagggcggt gatcgcttct actgtgttgt
     4141 cagctcgacg cagtccaagt tgcttgcacg cttctcgctg caatgcagga tctcccgatt
     4201 tgagatcctg tacgtaagcc tcatttgtct tctgggtaga ctgaccagaa aggcaggctg
     4261 caaaaataag aacggaaaat acgctccact tgctcatact gccagaggaa agctttgtac
     4321 ttactggtca atgcttattg ggccccggag ggcccggatc agggcttctt atagagaatt
     4381 cggaagcgcc gaatgcccgg atctgtgcct tccacgtagt cctcgaatgt ctccgtttga
     4441 agcaccgcgt cgagagggga gagatccgcc ctggacagag gccagggtgg gtgatcggcc
     4501 aggggctgtt cgttttctct acctcttgcg accaacagga gccttccccc gggattcatg
     4561 aactctggga gcctggccac tgctcgtgat ctcatgtcca gtgggagagc ctggatcgtg
     4621 taggactcaa acacaaagtc gcatgacgtg gccagctcga acaaattggc agtcatatac
     4681 tttacttttg tattcgcgaa ccgttctttt gcccaggcga tggcagtttc agaaatgtca
     4741 aatgcggtga cttccggaac aaatcgagag ataaattctg catcatctcc cagaccgcat
     4801 ccaaccacaa gagctgtctt cgccgatccc agtacttcct ggccgcttgc agtctttgcc
     4861 cacgatctta gattcggatt tgcttcatca tctgcccatg ggatgagatc agcgtttctt
     4921 gccgcgtcgc gatagagatc ttcgaaccat ccgagaggtt tctctttcgc acgtgcgatg
     4981 tgtttatctg ctggattatt ttcaacttgc atactatcct cactttattg tagattctca
     5041 acaatgcgaa atcgcatctg cattcttttt tgttctttca ctttcttgat tcttcagcac
     5101 tgctcctctc cgcagaagtc tgaacctctt ccatatgaat ggatgattct cacttccaca
     5161 aaagatcttc cgccgttcgc tggcgctgag attctggttc gtcatacttc ctctggttcg
     5221 atcggttggg ttctcaatgg agagagaacc acaattcaca aacttggtct gaacggcctg
     5281 ggccttccac cagatcttgt cgttcgcatg ggtggcccgg tggaaacagg aaagatcagc
     5341 tttctgtatc gtgatcctgg aacggatgct gttcattttt caagatacct cccgactttg
     5401 attgaaatcc ttaagagcga acccgcaaac gtagttgtct ttgctggata ttccggctgg
     5461 gcggaagacc agcttgcatc tgaaattcgc cggggcctct gggttcgttc ttccaaaccc
     5521 cggcgaagca tttttgttcc agagacttag aagagtccat gttctgtccc aaaatttgga
     5581 agactcattc ctttttcgcg cgcagcgtct ttcgcattca acaattctgc aagcgtgtcc
     5641 cattttccgg tggtgaactg cttgcgaaca ccttcgtcca tctgcattgc aaaagtctgt
     5701 gaacctgcac ttactctgga ttgaatcaga tcgatcgtga atgactctcc tgctttcgca
     5761 agcttgcgtg cttctgcgag atctgcggca ctcatcgtga aggccgtcac tcccacagtc
     5821 ctgcaatttc caagaaagat ctctccaaac gattccgcgg cgatcgcctt gatgcctctt
     5881 cgcgccagag attgcactgc atgttcgcgc gaagacccgc atccaaaatt gctttctgcg
     5941 atcaagaata ccgcgtctga attttttgga tcgtcgaagg ggtgcagatc acctctgcgc
     6001 gaaaatgctt tgcgttcatc ttcaaaagca tgttcctcga gcccgctaaa cacgatggac
     6061 ttcatgaatc ttgccggaag aatgcgatcc gtgtccacgt catttccagc aactaccatt
     6121 ccttttcctt ttactgtttc gattttcatt tcacacctcc agtgaattca cgcacgtcac
     6181 atacatatcc acggagcgcc gctgccgcta ccatggctgg cgacatcaag agagttcttc
     6241 ctgtgggaga gccctgtctt cctttgaagt tgcggttgga agagctggcg cacacctgtc
     6301 ctgcttccaa tttgtctgaa ttcatggcca ggcacatgga acatcctggt tgtctgaatt
     6361 caaatccagc atccgtgaac acgcggtcga ggccgagcgc caccgcttcg ttgtagacgt
     6421 tatgcgaccc tggcacaacc actgctttta catgcgctgc ctttgtagtt
//
LOCUS       DAOQJL010000172         6461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6709_length_6461_cov_4.677178, whole genome shotgun sequence.
ACCESSION   DAOQJL010000172 DAOQJL010000000
VERSION     DAOQJL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6709_length_6461_cov_4.677178"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1199
                     /locus_tag="PLG78_09085"
     CDS             144..1199
                     /locus_tag="PLG78_09085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23310.1"
                     /translation="MSFSLPDEIQHYEKKIYDQRQLIEISRALNSTLDYNYLIQAILD
                     ICLAQVQTMQAALFLMPDMDSDFIGPVDGYRGFVFTKPEEEYRIQVQSPLVQLMETTN
                     KSLKLAELDERISSHQPVPSLRELGVELMVPLKAKGKVIGLILLGEKAVGGDYPDNEK
                     SFLFDLASLGGIAVENARLYERATVDMMTSLKNHAYFQSRFREEREKALKRRHPLSLL
                     LTDVDKFKNFNDTHGHQAGDEVLKAVARVLIESARRTDFAARYGGEEFCLVMPGADEK
                     TALAMGEQIRQTVEAMTVIHEGKEMKVTMSIGVSCFDPDLDARSNKTMIERSDKALYA
                     CKHGGRNQVRAYSKDMG"
     gene            1271..1438
                     /locus_tag="PLG78_09090"
     CDS             1271..1438
                     /locus_tag="PLG78_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23311.1"
                     /translation="MPKLLELERLARQIDPDLECLVEYRGEDDRTGIQFRDCIVDYTA
                     CQNQTCIKKLM"
     gene            complement(1440..1523)
                     /locus_tag="PLG78_09095"
     tRNA            complement(1440..1523)
                     /locus_tag="PLG78_09095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1487..1489),aa:Leu,seq:caa)
     gene            1586..2227
                     /locus_tag="PLG78_09100"
     CDS             1586..2227
                     /locus_tag="PLG78_09100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNE23312.1"
                     /translation="MIFRADLTAHVELQEHETRHARARRLTDGAPISLGDGLGRRVRG
                     QLTSLRTAQFDLTTVETQFEPSRILYSAVPEPSRMEWLLQKGTELGMTAFVPVRFVRS
                     ENRSVRDDRARRIIEEAAVQSRRFVLPKMHPAMDLEEACKVSKNPIVLLPEAEKLLAT
                     EGSTEEISFFVGPEGGFSPDEISLLSKFSQRRLSRTILRVETAAICALSRCLL"
     gene            2224..2874
                     /locus_tag="PLG78_09105"
     CDS             2224..2874
                     /locus_tag="PLG78_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNE23313.1"
                     /translation="MTIRFVLASASPARRKLLDSAGIPHIAMASDFDEDSVKAEPMLF
                     VQILAKAKAETILAKIDSPDGSTHLPGLADNFFVLGCDSVLVLDGEIHGKPRDRSEAV
                     GRWRQMRGRSGELLTGHALIRNDRGQRRTLVKVQRTVVRFAQLEDSEIEAYVDAGEAM
                     KCAGAFALEGRGGLFVEGIEGCHSNVIGLSLPLLRKMMLELGAAPHEFWVSSAEEL"
     gene            2871..4040
                     /locus_tag="PLG78_09110"
     CDS             2871..4040
                     /locus_tag="PLG78_09110"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNE23314.1"
                     /translation="MKSENFETIAVRTQAERSGFREHSAPIYMTSSFMFEDAEQARAL
                     FADEIEGNIYSRFSNPNTTEFIQKICKLEGAEDGIATASGMSAIFTSMMPFLSSGDHI
                     IVSRSVFGSTHQIVTQILPRWGIEYTYVDILDTVGWEKAFRKNTKMVFVETPSNPALD
                     MIDLAWLSGLCKSHNVLLNVDNCFATPYLQKPISFGADIVMHSATKFIDGQGRTIGGV
                     IVGKKEIIAKVRFFARHTGPSMAPMTAWILSKSLETLAVRMDRHCQNALALARFLETH
                     PKVEKVIYPFLESHPQLELAKKQMRLGGGIVTFLVRGGIEAGRKFLDATEGLSFSANL
                     GDTRTIVTHPASTTHSKLSDEERAAVGIHPGTIRVSAGLEHIDDIISEIDRMIKA"
     gene            complement(4030..4941)
                     /locus_tag="PLG78_09115"
     CDS             complement(4030..4941)
                     /locus_tag="PLG78_09115"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNE23315.1"
                     /translation="MLKDKILKRQSGITLYGITPPKASTAQEKLSEISDAQVQRLSSL
                     SIDGLIVYDLQDEGSRTTENRPFPFMETLDPFSYARKYLEKLRVPKVIYRAVGKYSAD
                     ELTHFMEHAPPDEYLSVFVGAASKTQKVNLTMREAYALKKKIKSELLVGGVVIPERHS
                     AHSPEHLRVFDKISSGCKFFVSQGVYNLDASKNFLSDYYYYGKEHKIELVPIIFTLTP
                     CGSLKTLQFMQWLGISIPKWLENDLIHSNDILSRSIELSRNIWLELKSFADAKNIPIG
                     RNIESVSIRKEEIDASVELLKITSATP"
     gene            complement(4972..5382)
                     /locus_tag="PLG78_09120"
     CDS             complement(4972..5382)
                     /locus_tag="PLG78_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE23316.1"
                     /translation="MEIELTRKNDGYLFEAKNESGNTVMIDASPAIGGSGGGARPMEV
                     VIMALGGCSSIDIVNILKKQRQEITSFQVKIHAERREGETPSLFKSIHVDYYIGGVDS
                     DKAQRAADLSIEKYCSVSKLLEPTVKITYAVHSV"
     gene            5413..5946
                     /locus_tag="PLG78_09125"
     CDS             5413..5946
                     /locus_tag="PLG78_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE23317.1"
                     /translation="MSTECARQREEPMLESYWPFYYGGLAIAAVALLVLSIGGDYLAI
                     TRGYVSVCSIFTRRPYFNTPDIGGPFGFRTMFTIGVVVGGFIAAIVSGGFKPTFAFGT
                     FDSVWGTSMAVKGAVLVSGGICWGYGSRMAGGCTSGNSISGISRGSLASIVATVCFLV
                     GGAIVVHILEYVVRGKL"
     gene            5946..6347
                     /locus_tag="PLG78_09130"
     CDS             5946..6347
                     /locus_tag="PLG78_09130"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE23318.1"
                     /translation="MLRLFYLLAGVAFGFVLIYSGVSNYNVLYDMFLFRSFHMYGLLG
                     VAVGLSFVGVQILKKLKWKSVITGQDLKFETLMPTRAHVIGGLISGAGWGLTGACPGP
                     ALAQVGFGTLAGIFTVCGIFIGVYLFGRGKG"
BASE COUNT         1532 a         1488 c         1702 g         1739 t
ORIGIN      
        1 cctgtgacct ttgggttatg agcccaacga gctaccaact gctctaccgc gcggtgttat
       61 gactaggatt cgaaaagcgg ggcttcttgt ccatcatttt taggtgttga cccttgggca
      121 ggcgtgtata aaccttatcc gccatgtcgt tctcacttcc agatgaaatt cagcactacg
      181 aaaagaagat ttatgatcaa cggcagctca ttgagatttc cagagcgctc aattctactc
      241 tagattacaa ttatctcatt caagcaattc tcgacatttg tctggctcag gtccagacca
      301 tgcaggctgc tctgtttctg atgccggata tggattccga ttttatcggt cctgtggatg
      361 gctatcgcgg ttttgttttc acaaagccgg aagaggaata tcgcattcag gttcagtctc
      421 ctctcgttca actcatggag accacaaaca agtctctgaa actcgcagaa ctcgatgagc
      481 gcatctcatc gcatcagcct gtgccttctt tgagagagct gggcgttgaa ctcatggtcc
      541 ctctgaaagc aaaagggaag gtgattggtt tgattctcct cggagaaaaa gcggttggtg
      601 gagattatcc ggacaacgaa aaaagcttcc tttttgatct ggcatcgctc ggcggcatcg
      661 cggttgaaaa cgctcgtttg tatgaacgag ccacagtaga catgatgacc agccttaaga
      721 atcatgcata cttccagagt cgctttcgcg aagaaaggga aaaagctctc aagagaagac
      781 atcctctctc tcttcttctt acggacgtag ataagtttaa gaatttcaat gacactcatg
      841 gtcaccaggc aggagacgaa gtgctcaagg ctgtggccag ggtactcatt gaatctgcgc
      901 gtagaaccga ttttgctgcg agatacggcg gggaagaatt ctgtctcgtg atgcctggtg
      961 cggatgaaaa aacggcgctc gccatgggtg aacaaattcg ccagaccgtg gaagcaatga
     1021 ctgtgattca cgaaggcaag gaaatgaagg tcactatgtc gatcggtgtt tcctgctttg
     1081 atccggatct ggacgccaga agcaataaaa ccatgattga acgctcggat aaggctcttt
     1141 acgcttgtaa gcatggcggc cgaaatcagg tccgggccta cagcaaagac atgggctgat
     1201 ccgataatct aactacatac agaagggcca aaaggtcctg gcacgaatga acgtccacgg
     1261 agggatgttt atgccaaagc tcctcgaact agaaaggctg gcacggcaga tcgacccgga
     1321 tctggaatgc ctggttgaat accgcggtga agatgacagg actggaatcc agttccgtga
     1381 ttgtatcgtg gattacacgg cctgccagaa tcagacctgc attaagaagt tgatgtgagt
     1441 accgaaggtg ggacttgaac ccacaaggga ttgctcccgg tggattttga atccactgtg
     1501 tctaccaatt ccaccacttc ggcgtagaat caacgtcccc gcacggcctg tactgtaaac
     1561 catttatgca tcagttatta tgtttttgat ttttcgcgcc gatctcactg cgcacgtaga
     1621 gctgcaggaa catgagacca ggcatgcgcg tgcacgacgg ctaacagacg gcgcacccat
     1681 tagcctggga gacggtctgg gacgccgcgt gcgcggacaa ctcacatctc ttcgcaccgc
     1741 tcaatttgac ctgaccactg ttgaaactca attcgagcct tcgcgcattc tgtactctgc
     1801 cgttccagaa ccttcgagga tggaatggct tctacaaaag ggaacagagc tggggatgac
     1861 cgcgtttgtt ccggttcgtt ttgttcgttc ggaaaaccga tctgtcagag acgatcgtgc
     1921 gcgaagaatc atagaagaag cagccgttca atcccgtcgc tttgttcttc caaagatgca
     1981 tcctgcgatg gatctcgagg aggcatgcaa ggtttcgaaa aatccaatcg tgcttttgcc
     2041 cgaggcggag aaacttctcg cgacagaggg atctactgag gagatttcct tcttcgttgg
     2101 tcccgaaggt ggattctcgc cggatgaaat ctctcttctt tccaagtttt ctcagcgcag
     2161 gttgagtcga accatacttc gcgtagagac agccgcgatc tgtgcacttt cacggtgtct
     2221 gttatgacga ttcgctttgt acttgcatcc gcttctcctg cgaggagaaa gctgctggat
     2281 tcggccggga ttccgcacat tgcgatggcc agtgattttg atgaagattc ggtgaaagcg
     2341 gaacccatgc tgtttgtcca gattctggcg aaagcaaaag cagagacgat tcttgcgaag
     2401 atcgattcgc cggatggatc tacccacctt cctggcctgg cggacaactt cttcgtgctt
     2461 ggctgtgatt ctgttttggt tctggacggt gaaatacatg gaaagccgcg agatcgaagt
     2521 gaggccgtgg ggcgatggag acagatgcgc ggtagaagtg gtgagctttt gaccggtcat
     2581 gctttgattc gaaacgatcg cgggcagcgc agaactcttg taaaagtgca gcgtacagtg
     2641 gttcgattcg cacagcttga ggattcagaa attgaggcat acgtggatgc gggtgaggca
     2701 atgaagtgcg ccggtgcttt cgctctggaa ggaagaggcg gactttttgt tgaaggaatt
     2761 gagggttgtc actctaacgt aattggcctg agtctaccgc tcttgcggaa gatgatgctg
     2821 gaactgggcg cggcgcccca tgagttctgg gtgagttctg cggaggaatt atgaagagtg
     2881 aaaattttga aacaatcgcg gtgcgcactc aggcggagag aagtggattt cgcgagcatt
     2941 ctgcgcccat ttacatgact tccagtttta tgttcgaaga tgcagagcag gctcgcgctt
     3001 tgtttgcgga tgaaatagaa ggaaatattt acagcagatt ctccaacccc aacacaaccg
     3061 aattcattca aaagatatgc aaactggaag gggcagaaga cggaatcgcc actgcttccg
     3121 gaatgtcagc gatcttcaca agcatgatgc cttttctaag ctcgggggat cacatcattg
     3181 tatcgcgatc tgtgtttggt tctactcatc agatcgtaac tcaaattcta cctcgctggg
     3241 gaatcgaata cacctatgtg gatatcctgg acactgtggg ctgggaaaag gcatttcgca
     3301 agaataccaa aatggttttt gtggaaactc catccaatcc tgctctcgac atgattgacc
     3361 tggcatggct ctccggcctg tgtaagtcgc ataacgttct gctcaatgtg gacaattgtt
     3421 ttgcaacgcc ttatctacag aaaccgattt cgtttggtgc ggacattgtg atgcattcag
     3481 ccaccaagtt catagacggg cagggaagaa cgatcggcgg cgtcatcgta ggcaaaaaag
     3541 aaatcatagc aaaggttcgg ttcttcgcca gacacacagg tccttccatg gcgcccatga
     3601 ctgcgtggat tctttcgaaa agcctcgaaa ctctcgcggt gaggatggac cgtcattgcc
     3661 agaatgcact ggcacttgct cgttttctcg aaacacatcc aaaagtggaa aaagtcatct
     3721 atccattcct cgagtcgcat cctcagctgg agttagcaaa aaagcagatg aggctgggtg
     3781 gtggaatcgt tacgtttcta gtgcgcggcg gaattgaggc aggccgcaaa ttcctggacg
     3841 caaccgaagg tctttctttc tccgcaaatc tgggagacac gcggacaatc gtaactcatc
     3901 ctgcttctac aactcattcg aaactatccg acgaggagcg ggcggctgtt ggtattcatc
     3961 ctggaacgat tcgagtttca gcgggccttg aacacataga cgatatcata tccgagatcg
     4021 atcgtatgat caaggcgtag cgcttgtaat cttcaaaagc tcaacagagg catctatctc
     4081 ttccttccgg atggaaacac tctcaatgtt tcttccaatc ggaatgtttt tggcatcagc
     4141 gaagcttttc aactcaagcc agatgttcct ggacaattca atggacctgg aaagaatatc
     4201 gtttgagtgg atgaggtcat tttcaagcca ttttggaatg ctgatcccaa gccattgcat
     4261 gaattgcagt gtcttcaaag agccgcacgg agtcagcgtg aagataatcg gcacaagttc
     4321 gatcttatgc tctttgccat agtagtaata gtctgacaga aagtttttgg atgcgtctaa
     4381 gttgtatacg ccctgggaca cgaagaattt gcacccggat gagattttgt caaagactct
     4441 cagatgctcg ggcgagtggg cggaatgcct ttcagggatg actacaccgc cgaccaacaa
     4501 ctcgctcttg attttctttt tcaaagcgta agcttctctc atcgtcaggt ttactttttg
     4561 tgttttggaa gccgcgccaa caaagaccga caggtattca tccggcggag catgttccat
     4621 gaaatgagta agttcatccg cgctgtattt acctaccgcc ctataaatga cttttgggac
     4681 tctcagtttt tctaagtact tccgagcgta ggaaaaagga tcgagggtct ccatgaatgg
     4741 aaagggccga ttctctgtgg ttctagatcc ttcatcctgc aaatcgtaaa caatgagacc
     4801 gtcaatagag agggaactca gtctttgaac ttgcgcgtcc gaaatctcgc tgagtttttc
     4861 ctgagctgta gatgcttttg gtggggtgat cccgtagagt gtaattccgc tctggcgttt
     4921 aagtattttg tctttcagca tgattgcggt ttgtttatcg cataactcct gttaaacgga
     4981 atgaaccgcg tatgtgattt tcacggttgg ttcgagcagt tttgatacgg agcagtactt
     5041 ctcaatagaa agatctgccg ctctttgtgc cttgtctgag tccacgccgc caatatagta
     5101 atctacatgg atggatttga agagggatgg agtttcccct tcgcgtctct ccgcgtgtat
     5161 tttgacctgg aagcttgtga tttcctgccg ttgttttttg agtatattta caatgtctat
     5221 tgagctacag ccgcccaggg ccataatgac cacttccatg ggtcgtgcgc cgccacctga
     5281 accgccgatg gctggagatg cgtcgatcat gactgtgttg ccagattcgt ttttcgcctc
     5341 aaaaaggtag ccatcgtttt ttcttgtcag ttctatttcc attgtaatcc ttccatattc
     5401 gcgaccgggc aggtgtcaac cgaatgcgcc cggcaaagag aggagcctat gcttgaaagt
     5461 tactggccgt tttactacgg cggcctggcc atcgcggccg tcgcccttct tgtcctatcc
     5521 attggaggcg actaccttgc gatcactcgt ggatatgtga gtgtttgttc gatctttaca
     5581 cgtcggccct atttcaacac gcccgatatt gggggcccgt ttggctttcg cactatgttt
     5641 acgattggag tcgtggtggg aggattcatc gctgcgattg tatcgggtgg tttcaaacca
     5701 accttcgcat ttggcacttt tgattcggtc tggggaacgt cgatggcagt gaaaggtgca
     5761 gttcttgttt cgggaggaat ctgctggggt tacggatctc gcatggcggg aggatgcacg
     5821 tctggaaatt caatttcagg aatttcgcgt ggttctcttg cgagcattgt tgcgaccgtt
     5881 tgtttcctcg tgggaggagc aattgtagtt catattctcg aatacgtggt gcgggggaaa
     5941 ctgtaatgtt gagactcttc tatctacttg ccggcgttgc gtttggtttt gttctcatct
     6001 actccggtgt atccaattac aatgttctct atgatatgtt tctgtttcgc agctttcaca
     6061 tgtatggcct gctgggtgta gctgtaggcc tttccttcgt gggcgttcag attctaaaaa
     6121 aactcaaatg gaaatcagtg atcacaggcc aggatcttaa atttgaaact ctcatgccaa
     6181 cacgtgctca tgtgattggt ggactgatct caggtgcagg ctggggtttg accggagcat
     6241 gccccggacc ggccctggca caggtaggtt ttggaacgtt agccggcatc tttacagtgt
     6301 gcggaatttt tatcggagtg tatctgttcg gcagaggaaa gggctaatcg tgccgggcgc
     6361 gtggtctgga tttgcgcaag cgattgtaaa gggagtccgg gcggctggcg cgggacttca
     6421 ctatgtttat cgaaaaggag atgaacataa gcactatgtt c
//
LOCUS       DAOQJL010000173         6446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6743_length_6446_cov_4.996558, whole genome shotgun sequence.
ACCESSION   DAOQJL010000173 DAOQJL010000000
VERSION     DAOQJL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6743_length_6446_cov_4.996558"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..778
                     /locus_tag="PLG78_09135"
     CDS             203..778
                     /locus_tag="PLG78_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23319.1"
                     /translation="MGFLQKFFRKKDDPDLFEYAARSQEEEPSKKRRFWFLLIPIVIP
                     ASFFAGFQLGKDNSCEEERATISAMADTGTTNPQTAPYQSFLGTYKVDVSGHQGLLYI
                     YSLPSGGPGATIHFQNWGRRVPEPLFAVQTAGNRITFVRACQAQRCQEIGAPGPFRQD
                     YQGTLSDDAKKLEGTYTGGQSASTWRAIRIR"
     gene            986..1894
                     /gene="lpxC"
                     /locus_tag="PLG78_09140"
     CDS             986..1894
                     /gene="lpxC"
                     /locus_tag="PLG78_09140"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNE23320.1"
                     /translation="MTVSATHPGLTKRATVRGIVHIDGIGLHSGEAANLRIKPAPAGT
                     GLVFQSPNHRGPIEVTPFNAVDTRQAVTLSNKNWKVQTVEHLLAALAATGVTDAILEL
                     DSQEVPILDGSSRIFVQEIQKVGVRELGAELEPIRLAQAVWVVDGDRYIVALPSDSFR
                     VTCTIDFPHPELRGQSISMDLDSRTFLEEVVDARTFGFLTDVEALRKKGLIKGASADN
                     AVVLTEDGYLDREGLRFSDECIRHKVLDLVGDLYLMGRPIHAHVIAHRGGHGLDVALA
                     RLILQRTAMDELSARRQQKADTVVAL"
     gene            1978..5226
                     /locus_tag="PLG78_09145"
     CDS             1978..5226
                     /locus_tag="PLG78_09145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23321.1"
                     /translation="MEALDFLNRHSIYLNYFAIGAIICFIFNFICGAFLLALPHKTKS
                     TTHMGIGLMFMSFFVFGYVISQSFYSDIAAYHRWVTVGFVLPAIVHFVQWLLRFPENN
                     KPGLTRWTGVAMWALSLGALALFMYASVGADRKFHFSAHYWDFDLEKASKYVGFLIFA
                     FVLCMPGFGIWKFVIVKTKERYAIVLIIASLLIASVIPAILNIQSRDGVVERGTFLLV
                     FVLTIVGGFFMVILTYMNTTKDRSTFMAKIVGITLVTFLVMMQGVSLVTMTDREEEYD
                     ALRVQYMLRALDGADVHPTIEYIVALEPVKKTIDQSYQSERYAQLPESKRVDFVAQTP
                     ALLNTRIFEEIQSASAQPQFRDAVANIVRDSHVEFAGYKAALEDAAKQAPNDDALKGS
                     INDYMARLDKEAFINTNRISGIPSDSHFREELMKHLGKANKYFAFFVPGIQEYMKQNP
                     DMPPVTLKKGILKYLARFSPDHTRVYRQSPDGNQHFVAFNVYDAGTGTMHEVGYSYQE
                     YRAFVHPAAKRLVFILLIVLVVLVVLYPLFFRGSLVNPLNSLLRGVTKVNQGDLTVEV
                     PVKVQDEIGFLTTSFNSMVASIKDARAKLEDYAANLEEKVQARTAELNKTLEVVHALK
                     IQQDGDYFLTSLLAKPLFFNANKSRSVTTEFLLRQKKHFEFRNKQSELGGDICITGNL
                     RFSKHPERRYVVSLNGDAMGKSMQGAGGSLVMGVVMNSILARSAKNNRVLTISPEQWL
                     KDVYEEVHSVFLAFNGSMVISVVMSIVDEVTGEMWYWNAEHPFQILYRNGKASFIEED
                     LQLRKVGLESEIPFRVRNFQLQPGDVVLMGSDGRDDIDLTPDASTRTINEDEFLILKR
                     VEESRADLNTILERLLATGELTDDLSFLRIEFKGVTAAGTSGPTESKPVAMAAPERVI
                     IDIDPDDEDDMNFEDVEELGSFEELFQEGRRLARQGKHEEALELLRQAYAMRRDVPAL
                     NKILAVLTFKDRDYPKAIEILGNYLEHDPHVADFWLYLSIAHKRMGEYERSLEAAQKV
                     YEINPNRIPNLINMADLYQKLGNASRAQELLDRALQLDPGNRQAQSLLTVIS"
     gene            5228..5773
                     /locus_tag="PLG78_09150"
     CDS             5228..5773
                     /locus_tag="PLG78_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE23322.1"
                     /translation="MEQKRSARRTSLRYKFQLRLVRRAMLPLAVLSAAAMVVIWSATT
                     GRHEYSMDELRWLGIGGVALLFVASLVGVGWSLMLYSSRLAGPLVHLEKTLAAIAAGD
                     LSTRVHLRKDDELQEHAAHLNRTFMSLQDRVKRIHQYCRYARETVDRLAASPDVKDKD
                     QLDQMRQLIGFIEEALQDFKV"
     gene            5776..6273
                     /locus_tag="PLG78_09155"
     CDS             5776..6273
                     /locus_tag="PLG78_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23323.1"
                     /translation="MVMRLDDVLQRIRTLDRDVEELRRLRARLPANRAFTSGMQVAFD
                     RHINELLNEKIQLEELEVEHIPEDLLNDIASVDVATASRITVDREKKEYELTSEDKKI
                     MEFIRAMPKTEIHLHMEACINQQTLQGMLDKNGVPYESEQLKKLYQFTNLQEFIKLFL
                     FIFNM"
BASE COUNT         1586 a         1627 c         1614 g         1619 t
ORIGIN      
        1 agattccatt catcatgaaa ctggggagcc aagggcatca tgatcacgaa atccaggatg
       61 ttcgtaaatt gcatgcctgc aaggatcagc aggatggatc tttcgtgtgc ttcgggagac
      121 atcgggtcaa actcgcggga ttgcgtttcc agtcacgaac ataaggctca atctgttctc
      181 ttttgagccg atgagaagat acatgggctt tctccaaaaa ttcttccgca agaaggatga
      241 tccagacctg ttcgagtatg ccgcacgctc tcaagaagag gaaccaagca aaaagcggcg
      301 attctggttc ctgttgattc caattgtaat tccggcttct ttttttgccg ggttccagct
      361 tggtaaagac aacagttgcg aagaagaacg agcgaccatc agtgcgatgg ccgacactgg
      421 cacgaccaat ccacaaacag cgccttacca gtcgtttctc ggaacctaca aggtagacgt
      481 cagcggacat cagggtctcc tctatatata ctctctccct tccggtggtc ctggagccac
      541 gattcatttt cagaactggg ggcgtcgtgt tccagaaccc cttttcgcag tccaaacagc
      601 cggcaatcgg atcacatttg tgcgtgcctg ccaggcgcag agatgccagg agattggcgc
      661 tccagggccg ttcaggcagg attatcaggg aactctctca gatgatgcca aaaagctgga
      721 aggaacatac acaggcgggc agtcggccag cacctggagg gcgattagaa tccggtagaa
      781 gcctgtccag ggtcgtgttg cagaaccgca tcactgagtg cgcccttact acaatcggga
      841 cagattatgt ctcattcccg gcaatggctg accaaggccg atttgcggtc tcgcccccaa
      901 aatctgcaca gaatggccaa attgcggcgt tttttgtgaa agggctcccc tggtacgggt
      961 cttgcgttag ggggggcaga agcctatgac tgtatctgca acacatcccg gtctgacgaa
     1021 acgtgccact gtgcgcggca tcgtccacat cgacggcatc ggcctgcatt caggtgaagc
     1081 cgccaatttg cgcatcaaac cagctccagc cggcaccggt cttgtttttc aaagccccaa
     1141 tcaccgtggc ccaatcgaag tgaccccttt caatgctgtg gacactcgtc aggctgttac
     1201 tctgagcaat aaaaattgga aagttcagac ggtagagcat cttctcgcgg cccttgcagc
     1261 cactggcgtg acagatgcga tcctcgagct cgattcccag gaagttccta ttctggacgg
     1321 ctcttcgcgt atcttcgtgc aggaaatcca aaaagtaggc gttagagagc ttggagccga
     1381 actggagccg attcgccttg cacaggcagt ctgggtagtg gacggagatc gttatattgt
     1441 agcccttcct tctgactcat ttcgcgttac atgtaccatt gattttccgc atccggagct
     1501 acgtggtcag tccatcagca tggatctgga cagcagaacg ttcctggaag aagtagtgga
     1561 tgcgcgcact tttggctttt tgacagatgt ggaggctctc cgcaagaagg gactcatcaa
     1621 aggtgcttcc gcagacaacg cagttgtgct cacagaagat ggttacctgg accgcgaagg
     1681 tcttcgcttt tcagacgaat gcattcgcca taaggtcctg gacctcgtag gcgatctgta
     1741 tctcatggga cgccccattc acgctcatgt aattgcgcac aggggcggcc atggattgga
     1801 tgttgctctg gctcgtctga tcctccagcg cactgcaatg gacgaacttt cagcaagaag
     1861 acagcaaaaa gcagacacag ttgtggctct gtaggcctct aaaaatactg ttgacactcc
     1921 ccgatttgcc gaactataaa tgatggatcg gccgttcccg gtccatgggg agttgcgttg
     1981 gaagccttag attttctcaa tcgccacagt atttacctga actacttcgc cattggcgcg
     2041 atcatctgct tcatctttaa cttcatttgt ggagctttcc tcttagcgct cccacacaag
     2101 acaaaaagca caacgcacat gggcattgga ctcatgttca tgtccttctt tgtttttggc
     2161 tatgtaatca gtcagtcttt ctatagcgat atcgctgctt accatcgctg ggtgactgta
     2221 ggctttgttc ttccagctat tgttcacttt gtgcaatggc tccttcgctt tcctgaaaac
     2281 aacaaaccag gtctgactcg ctggaccggg gtggccatgt gggctctttc cctcggtgct
     2341 ctggctctct tcatgtatgc gagcgtaggt gcagatcgta agtttcactt ctctgctcac
     2401 tactgggact ttgatctgga aaaggccagc aagtatgttg gattcctgat ctttgccttt
     2461 gttctgtgca tgcccggatt tggtatctgg aagttcgtga tcgtaaagac aaaggaacgt
     2521 tatgcgatcg tgttgatcat cgcatcactc ctgattgcgt cggtcatccc tgctattctc
     2581 aacattcaaa gccgcgatgg tgtagtggaa cgggggacgt tccttctcgt atttgtacta
     2641 acgattgttg gtggtttctt catggtgatc ctcacctaca tgaacacgac caaagatcgt
     2701 tccacattca tggccaagat cgttggtatc actcttgtga cgttcctcgt gatgatgcag
     2761 ggcgtgagtc tcgttaccat gacggatcgc gaagaggaat acgatgcgtt acgagttcaa
     2821 tacatgcttc gcgcactcga tggagcagat gttcatccaa cgatcgaata cattgtggcc
     2881 cttgagcctg tgaaaaagac catcgatcag tcgtatcaat cggaacgtta tgcgcaattg
     2941 ccggaaagca agcgtgtcga ttttgtagcg caaacaccgg ccctgctgaa cactcgtatt
     3001 ttcgaagaga tacagtccgc ctctgcacag cctcagttcc gcgatgccgt tgcgaatatt
     3061 gtgcgtgata gccacgttga gtttgccggt tacaaagcgg ctctcgagga cgcagcaaag
     3121 caagcgccca atgatgacgc gctcaaaggc tctatcaatg actatatggc tcgcctggac
     3181 aaagaggcgt tcatcaacac aaaccgaatc tcaggcattc cttccgatag tcactttcgc
     3241 gaagagctga tgaaacatct gggcaaggca aacaagtact ttgcattctt tgttccgggc
     3301 attcaggaat acatgaaaca aaatccggat atgccaccgg tgacactcaa gaagggaatt
     3361 cttaaatacc tcgcgcgatt cagtccggat cacaccaggg tctatcgcca gagcccggat
     3421 ggaaatcagc attttgtagc tttcaacgtg tacgatgcag gcaccggaac gatgcacgaa
     3481 gttggatatt cctatcagga gtatcgcgca ttcgttcatc ctgcggccaa acgcctcgtc
     3541 tttattttgt tgatcgttct ggttgttctg gtcgtcctct atcctctgtt cttcagaggg
     3601 agtcttgtca atccactcaa ttcacttctg cgtggtgtga ccaaggtgaa tcagggcgat
     3661 ctgacagtag aagtgccggt caaagttcag gatgaaatcg gctttctcac tacgtctttc
     3721 aactccatgg ttgcatccat caaggatgcg cgcgccaagc tagaagacta cgccgccaac
     3781 ctggaggaga aagttcaggc tcgcacggca gaattgaaca agaccctgga agtggtgcat
     3841 gcactgaaga tccagcagga tggggactac ttcctcactt ctcttcttgc aaaaccgctt
     3901 ttcttcaatg caaacaaatc tcgctccgtg accaccgaat ttctgcttcg tcaaaagaag
     3961 cactttgaat tcagaaacaa gcaatcggaa ctgggtggtg atatttgcat caccggtaac
     4021 cttcgcttct ccaagcatcc ggagcgtaga tatgtagttt cgctgaacgg agatgcgatg
     4081 ggtaaatcca tgcagggtgc cggtggatcc ctcgtgatgg gtgttgtgat gaactccatc
     4141 ctggctcgtt ccgcgaagaa caatcgtgta cttacgattt ctccagagca atggttgaaa
     4201 gatgtgtacg aagaagttca ttccgtcttc cttgcattca acggatcgat ggtgatctcg
     4261 gtcgtcatgt ccatcgtgga tgaagtgaca ggcgaaatgt ggtactggaa cgcggagcat
     4321 cctttccaga ttctctatag aaatggaaaa gcctccttca ttgaagaaga cctccagttg
     4381 cgaaaagtag gtcttgagtc agaaattcca ttccgcgttc ggaatttcca gcttcaacca
     4441 ggtgacgtgg ttttgatggg atcggacggt cgcgacgaca ttgaccttac tccagatgca
     4501 tccacacgaa cgatcaacga agacgaattc ttaatcttga agagagtaga agaatctcgc
     4561 gcggatttga atacgattct ggaaagactc ctcgcaacgg gcgaattgac agacgacctt
     4621 tccttccttc gcattgaatt caaaggcgtg actgcagccg gaacttcagg tccgactgaa
     4681 tccaaaccgg tagccatggc tgcgccggag agagtgatca tcgacattga cccggacgac
     4741 gaagatgaca tgaacttcga agacgtagag gaactgggca gtttcgaaga acttttccag
     4801 gaaggtagaa ggctcgcacg tcagggaaag cacgaagaag cgctggagtt gctcagacaa
     4861 gcatacgcga tgcgaagaga tgttcctgct ctgaataaga ttcttgctgt gctcactttc
     4921 aaggatcgcg attatcccaa ggcaatagaa atcctgggca attacctgga acacgatccg
     4981 cacgttgctg atttctggct ttatctctcc atcgcgcaca aacgaatggg cgaatatgag
     5041 cgatcccttg aggccgccca gaaggtatac gaaatcaacc cgaaccggat tccaaacttg
     5101 atcaatatgg cggatctgta tcagaagctg ggaaatgcgt cgcgtgctca ggaactcctg
     5161 gatcgggcac tgcaacttga ccccggtaac cgacaggcgc agagcctgtt gaccgtcatc
     5221 tcctgacatg gagcagaagc gcagcgcaag acggacatcg ctccggtata aatttcagct
     5281 tcgcctggtg cgtcgggcca tgcttcctct tgccgtgttg tccgcggccg ccatggttgt
     5341 gatctggtct gcaaccaccg gtcgtcatga gtattccatg gacgaacttc gctggctggg
     5401 gattggcgga gtggctctgc tctttgtagc atctctcgtg ggtgttggct ggagtctgat
     5461 gctctattct tccaggctcg ccggtccact cgttcatctt gaaaagactc tcgcggccat
     5521 tgcagccggt gacctttcca ccagagtaca tctgcgaaag gatgatgagc tccaggaaca
     5581 cgcggctcat ttgaatcgga cgttcatgtc tttacaggat cgcgtgaagc gaatccacca
     5641 atactgcaga tacgctcgcg aaacagtaga tcgcctggcc gcgtccccgg acgtgaagga
     5701 caaagatcag cttgaccaga tgcgtcagtt gattggattc attgaagaag ctctccagga
     5761 cttcaaggta taaccatggt aatgcgactc gacgatgttt tgcagagaat tcgcacgctc
     5821 gatcgcgacg tcgaagagct ccgccgtctc cgggcccgtc tacccgccaa cagagcgttt
     5881 acttccggaa tgcaagtggc gtttgatcgc catatcaatg aattgctcaa tgagaaaatt
     5941 caactcgaag aacttgaagt agaacatatc cccgaagacc tcctcaatga cattgcttcc
     6001 gttgacgttg ctactgcgtc gcgaattacg gtcgaccgcg aaaagaaaga atacgagctc
     6061 acttccgaag acaagaaaat catggaattc attcgggcca tgcccaagac tgaaatccac
     6121 ctgcatatgg aagcctgcat caatcagcag acgcttcagg gcatgctgga caagaatggt
     6181 gttccatacg aatccgaaca actaaagaag ctttatcaat ttacgaattt gcaggagttc
     6241 atcaaactct ttcttttcat ctttaatatg taaaaccttt tcacaaagat ttttaacaat
     6301 ctcggtcaat tctgtttctt ttggatatgc ccaattgatt ctactggtta gattgtagaa
     6361 gtattgcttt gacataaaga tcattggcgt atagagttcc atattatcct ttaccgtgat
     6421 cttattccat accaaacgga aagcca
//
LOCUS       DAOQJL010000174         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6843_length_6380_cov_4.788458, whole genome shotgun sequence.
ACCESSION   DAOQJL010000174 DAOQJL010000000
VERSION     DAOQJL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6843_length_6380_cov_4.788458"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3979)
                     /locus_tag="PLG78_09160"
     CDS             complement(<1..3979)
                     /locus_tag="PLG78_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE23324.1"
                     /translation="MRRVLFVCILLAACKTIPPVDPPEFDYPGLARLAETAPGRTIPL
                     TIQRGANLHPVSSQTGFLFYSSNRDGSGDIWMRDLHNTVAFPVIKHPAEQGKPAVPLN
                     GQKLAFVSFDTDAGGDIRIVEIKPDSIIKDSLAGSTPPNLWDSSDSLSEQVVQASKQL
                     PPACRGAFAETDPVFSGDGKYLYYVSDRCTPGTFNIWRIELKGIDLKGTPEIISQKGG
                     VQPALSYDGKRITYVSYRSGHAEIYVQDLQTRQEILVRTGGGILQYPALSPDGAIVFY
                     IAVREDTNQNGSLDAMDKAGIYSAPVAGGREARLVDPSTSLHGLYFSLFLGGTLLYSA
                     ESYQNISVYFVRPEGVIPRQADIEEQYLFASRFLREPGKRTIALDALERFFGGTKEFI
                     LYEGKYLQSRVDTLIGDERKAAEEELKEARARNPFAEYQLTLAALPRQKSRVEFIQEF
                     LKKTESSDLQNEQRTLIVPAVLDDLSKAQESEGDKPGALESLVRIDKEFPTYYRRARI
                     RLREAVLELKVTGKIPAVAMEELQAKPSIQRREEILTEIVNYFGRIRKPDDLDAAVSK
                     ELERADLIPLIRSTLEYLRARSLYDRGRDEEALTLARNLQTGLPQESGMYVRTWQLLA
                     FLFERRRDYAGAYEARLRYGGAYRPDTGVKIGLDEYREILEQSDRTIKRTLASARSIA
                     AVVDEQDAVISRTLLSRTLEGTPVQTLLNQVRQNPIELSASDREALHDLCFGNTGAII
                     GNLGKPEYVKTFVQFCKDAEPYLKGKNEGGMNLEFARTGSDLLYLVSYSNVNILNILF
                     YNMKRVNLFEKFHEENSIKYHRLKVDIAMERNRNRIEWDRRQVAILDPRNVIDLFSEG
                     DPFDATSFNEVVHGYKFAATEARSVGELSFVYGYAYALIQKSVERDRFYDRSAKEGIT
                     IPEDVRRAKKQDTLRDLKTAEYLLQYILFVKPDNADAYLQLGWLYQYIDDRRSRNIPT
                     AETFVVRVVNYLTRTSAPRATDGQYYIDIYRAYFPDRLYENNVELYRQAIQLIGFLNS
                     TDPALPDLHLNLANNYFQLLNFKKAIDHFNQNEDLRRAVGGASDPVRTGLFYFNRGRA
                     YYYEGQTTSASRDLKKALEMYTSSDLSPAEELVEGIRFRITDVGEKSENMARLKVRLE
                     DGQKRLDNARLRVAMIGALYALSLYESGRYAESSRAYETADAQIRRMQEPANELRAGI
                     LNFLALARQNQSRIESSDIQAIASRDLSKEIGLSRQDARYQPQSIGGRLLGCLLTYGE
                     DFSVIGEGRNPYGLAPLRQYELSLGILLENRILAGDRSGAFEILSQRREIFDGKDGDV
                     NLGRIG"
     gene            complement(3976..4602)
                     /locus_tag="PLG78_09165"
     CDS             complement(3976..4602)
                     /locus_tag="PLG78_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23325.1"
                     /translation="MKGLCIFLALWAFASCAFLQKNLKDPAVNLYRRGSCVSIDIPPI
                     TLTPARTAAERQLIGEDREIEKDGWLLASARSTTRAEESVVLQESRRIYREQAVLEFY
                     EESLREYARAGVLGEDTDRAEVVIVPERIAPRTARFRSPEEMDLARRTADEINKSRSY
                     IIQYYLKQEKEKPGSDVSLVEKSLRQSFLKNLRPGDWVMSNGNWIRQK"
     gene            complement(4599..>6380)
                     /locus_tag="PLG78_09170"
     CDS             complement(4599..>6380)
                     /locus_tag="PLG78_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23326.1"
                     /translation="SYNNATIRGNANIIPGEGVLVDLIVSRLRLAPFAGPSMDGVASG
                     QIRLQSPESFRDLDVKSELHVSETRLRMQDSRSAPLELGLVANGKIGTGDTTTVSMDK
                     LLLTVQNQSGVNLLTLNSATDMSFGELQNIQFHIKDLTVRYADLHSSLPGNLRYTLAP
                     YRSYLDDGVKVSGSFGMRGTKDVTNITTDMMLAMPGIHLMDLAVHTSVSLSEKKTLIE
                     DLKMTGLRGALLGTVKGSITKVDGKSVPDLTVNLGLSSKTLFAIHKNLALQGGLLMDF
                     KIQADSVRGNVNANDLSLEIYTDCDAQSACKKMRIEKMNLALPIFHDLKIARPALLAD
                     SPAGLTFDAAQFRGDANLTIQFIASTHNPRGAIVKDGYFYSGSLLPGRGPGVSASLEY
                     RKNVLYIKWLRYQIFRPKAREGNDVWVADGRIEGRDVFFNLADLSPRSMEFGGVMQIQ
                     NFDLEPYLPASRSNYDGVISAEMGFRGKDLGNALYNSDLRLSVYRLSREFSGFATRVV
                     MPGQVAGFIVKSALEIPSITLELQNGLVYTTIGIARKDVRSFTTALSFIMKPTGEEIR
                     QERIPLAQFLDRARSEVEAGVKVEERQ"
BASE COUNT         1550 a         1686 c         1538 g         1606 t
ORIGIN      
        1 aaccaatccg cccaaggttc acgtcaccgt ctttgccatc aaaaatctca cggcgttgcg
       61 aaaggatctc gaaggcaccg cttctgtctc cagctagaat tctgttctct aaaagaatcc
      121 cgagtgaaag ttcatactga cgaagaggtg ccagaccata cgggtttcta ccttccccga
      181 tcacagagaa atcctctccg tatgtcagaa gacaaccgag caacctccct ccaatcgact
      241 gcggctgata gcgcgcgtcc tgcctgctta aaccaatctc ttttgaaagg tcacgtgaag
      301 ctatggcttg aatatcagag ctttcaatcc gactttgatt ttgacgcgcc agtgccagaa
      361 aattgagaat gccagcccgc agttcatttg ccggttcctg cattcggcga atctgcgcgt
      421 ccgccgtttc ataagctcga gacgattccg cataacgtcc agattcatac aaagacagag
      481 catatagagc tccgatcatg gccacgcgaa gtcttgcgtt atccagtctc ttctgtccgt
      541 cttccagcct gaccttgaga cgtgccatgt tttcgctttt ttcacctacg tctgtgatcc
      601 tgaatcgaat tccctccacc agttcttctg ccggtgacag atccgaggaa gtgtacattt
      661 ccagtgcttt cttcagatcg cgcgaggcgc ttgttgtctg accctcgtag taataggcgc
      721 gaccgcgatt gaaatagaaa agacctgttc gcacgggatc agaggccccg cccacggcac
      781 gcctgagatc ctcattctga ttaaagtgat caatcgcttt tttgaaattg agaagctgga
      841 aataattatt cgccaggttc aagtgcaggt cagggagcgc ggggtccgtt gaattcaaga
      901 aaccaatcag ctgaatggcc tgcctgtaaa gctctacatt gttctcataa agacgatcag
      961 ggaaataggc gcggtaaata tctatgtagt actgaccatc tgtggcacga ggagcgctgg
     1021 ttctggtcag atagttgacc acccgaacca cgaacgtttc cgccgtagga atgtttctac
     1081 tccttctatc gtcaatgtac tgatacagcc agccaagttg caaataggcg tccgcgttgt
     1141 ccggcttcac aaacaagatg tactgcagca aatattctgc agttttcaag tcgcgtagag
     1201 tgtcctgttt ctttgctcga cgcacatcct ccggaatggt gatgccttcc ttcgcgctgc
     1261 gatcgtagaa gcgatctcgc tctactgatt tctgaatcaa ggcatacgcg tatccatata
     1321 caaaactcaa ttcaccaaca ctgcgggctt ccgtagccgc gaacttgtat ccatgcacta
     1381 cttcattgaa ggaagttgcg tcaaacggat ctccttcgct aaaaaggtcg atcacattgc
     1441 ggggatccag aatcgccacc tgacgcctgt cccactcgat tcggtttcga ttgcgctcca
     1501 tcgcaatgtc caccttgagt ctgtgatatt tgatcgagtt ttcttcgtgg aacttttcga
     1561 agagattcac tctcttcata ttataaaaga gaatgttgag aatatttaca ttcgagtaag
     1621 ataccagata aagaagatca gatcctgtcc ttgcgaattc aagattcata cctccttcat
     1681 tttttccctt gagataaggc tctgcatctt tgcagaattg aacaaacgtc tttacatatt
     1741 ctggtttgcc caggtttcca atgatggcac ccgtattgcc aaaacacaaa tcgtgcagag
     1801 cttccctgtc agaagcggat agctcaatcg gattctgcct cacctgattg aggagggtct
     1861 gcactggagt tccttcgaga gtacgagaaa gaagtgttcg cgaaatgaca gcatcctgtt
     1921 cgtccacaac cgccgcgata gatcgcgcgg atgcgagggt gcgcttgatg gttctgtctg
     1981 actgttcgag gatctcgcgg tattcgtcga gaccaatttt gacgcctgtg tccggacgat
     2041 aggctcctcc atatcgcaga cgcgcttcgt atgcgcctgc atagtcacgt cgtctctcga
     2101 acaaaaacgc gagcaattgc caggttctca cgtacattcc agattcctgg ggaaggccag
     2161 tctgcagatt tcgcgcaaga gtgagtgctt cttcgtctct ccctctatca tagagacttc
     2221 gcgcccgaag atattcgagg gtggagcgaa tgagaggaat gaggtcggct cgttccagtt
     2281 cttttgatac agcggcgtcc agatcatctg gctttctgat tcttccaaaa taattgacga
     2341 tctctgtcag aatctcctca cgtcgttgaa tggatggctt tgcctgaagt tcttccatgg
     2401 caacggctgg aatctttccc gtgaccttca actcgaggac cgcttcgcgc aggcgaatgc
     2461 gcgctcgacg atagtaagta ggaaactctt tgtcgatccg caccaaagac tcaagtgcgc
     2521 ctggcttatc cccttcagac tcctgcgcct tacttagatc atcaagaacg gcaggaacga
     2581 tcaacgtgcg ctgttcgttc tgcaaatcgg aagattcagt tttcttcaag aactcctgga
     2641 tgaactccac tctgcttttt tgtctgggta aagcagccag agtcagctgg tattccgcaa
     2701 aaggatttcg cgctcttgct tccttcaatt cttcttccgc ggcctttcgc tcatctccga
     2761 tcagcgtgtc cacgcgcgat tgaagatact taccttcata tagaatgaac tcttttgtgc
     2821 cgccgaagaa tctctccagc gcgtccagag caatcgttcg cttccctggt tcgcgcagga
     2881 atctggaagc aaacagatac tgctcttcga tgtcagcctg acgcgggatc acaccttccg
     2941 gccgaacgaa atacacgctg atgttctggt atgactccgc agaataaaga agggtgcctc
     3001 ccagaaacaa agaaaagtaa aggccgtgca aagatgtaga tggatccaca aggcgggctt
     3061 cgcgaccccc ggcaacaggc gcagaataga taccggcctt gtccatggca tcgagtgatc
     3121 cattctgatt tgtgtcttcg cgaactgcga tgtagaacac gatggctccg tcaggcgaaa
     3181 gagcgggata ctgaagaatg cctccccctg ttctaaccag gatttcctgt cttgtctgca
     3241 aatcctgtac ataaatttct gcgtgtccac tgcgataaga gacgtacgtt atgcgtttgc
     3301 catcataaga aagtgccggc tgcactccgc ctttctgcga gatgatttcc ggggtcccct
     3361 tcaaatctat gcccttcaat tctattcgcc atatattaaa ggtccctgga gtgcagcgat
     3421 cgctcacata gtagagatac ttcccatctc cggaaaacac tggatccgtt tcggcgaacg
     3481 caccacggca ggcaggcgga agctgtttgg aagcctgcac tacttgttcg gagagactgt
     3541 cagacgaatc ccagaggtta ggcggcgttg atccagccag actatctttg atgatcgagt
     3601 ctggtttgat ttcaacaatg cgaatgtctc ctcccgcgtc ggtgtcaaaa gaaacgaatg
     3661 ctagtttctg tccattgaga ggaactgcag gcttgccctg ctccgccggg tgcttgatca
     3721 ctggaaatgc aactgtgttg tgcaaatcgc gcatccatat gtcgccggat ccatccctgt
     3781 tggatgaata gaacaaaaag ccagtctgag aactgactgg atgaagattg gcccctctct
     3841 gaatggtcaa aggaatggtg cggccgggtg ctgtttccgc gaggcgagca agaccgggat
     3901 aatcaaactc cggcggatcc accggaggga tggtcttgca tgcagcaaga aggatgcata
     3961 caaagagtac gcgtctcatt tttgcctgat ccagttaccg ttgctcatca cccagtctcc
     4021 cgggcgaagg tttttgagaa aggattgtcg cagggacttt tccaccagcg aaacgtccga
     4081 gccgggcttt tctttttcct gttttaagta gtactgaata atataagatc tggatttatt
     4141 gatctcatct gccgtgcgac gcgccaggtc catttcttcc ggacttcgaa aacgagccgt
     4201 acgcggtgca attctttctg gaacaatcac gacttctgct cgatccgtat cttctccaag
     4261 gaccccggcg cgtgcatatt cccggagcga ttcttcataa aactcaagta cagcctgctc
     4321 gcgatagatg cgtctggatt cttgcaggac cacggattct tcagcgcgag tggtgcttcg
     4381 cgcggaggcc agaagccatc cgtccttctc aatttcgcgg tcttctccaa tgagctggcg
     4441 ctccgccgcg gttctagccg gagtcagagt aataggtgga atatcaatgg agacacacga
     4501 tccccggcga tacaggttca ctgccgggtc cttcaagttc ttctgcaaga acgcgcagga
     4561 cgcaaacgcc cagagggcca gaaaaatgca aaggcctttc attgccgttc ctccactttc
     4621 acgccggcct ccacttcgct tctggctcga tccagaaatt gcgcaagcgg gattctttcc
     4681 tgacggattt cctcacccgt tggcttcatg atgaagctga gtgctgtagt aaaactgcgg
     4741 acatcttttc gcgcaatccc gatagttgta taaactagac cattctgaag ttccagtgta
     4801 atggatggaa tctcaagcgc actctttact ataaaacccg caacctgccc cggcatcact
     4861 acgcgcgtcg caaaaccact gaactccctg ctgagacgat acacggacag gcgaagatcc
     4921 gaattgtaaa gcgcgttgcc caggtccttg ccgcgaaaac ccatttctgc gctgatgact
     4981 ccgtcgtaat tggagcgcga agctggaaga tatggttcaa gatcgaagtt ctggatttgc
     5041 atcacaccac cgaactccat ggacctgggt gacagatcag ccagattgaa gaaaacatct
     5101 ctgccttcaa ttcgcccgtc ggcaacccac acatcatttc cttcgcgagc tttcgggcga
     5161 aagatctgat acctcaacca cttgatatag agcacattct ttctatattc gagagaagca
     5221 gaaacgcccg gccctctgcc tggcagaagg gaaccagaat aaaaatagcc gtctttgacg
     5281 atcgccccgc gtggattgtg ggtggaggca atgaactgaa tggtcaggtt tgcatcgcca
     5341 cgaaattgcg cggcatcgaa cgtgagccct gccggagaat ctgcaagaag agctggccgg
     5401 gcaattttca agtcgtgaaa gatggggagc gcgagattca tcttttcaat acgcattttt
     5461 ttgcaggcgg attgtgcgtc acagtccgta tagatttcca gagacagatc attggcattc
     5521 acattgccgc gcacggaatc tgcctggatc ttgaaatcca tcaagagccc accctgcaat
     5581 gcaaggttct tatgaatggc aaataacgtc ttactggata ggccaaggtt gaccgtcaaa
     5641 tcaggcacac tttttccgtc tactttcgtg atggatcctt ttacagtccc aaggagcgca
     5701 ccgcgaaggc cggtcatctt gagatcctcg atcaatgttt tcttttccga cagtgaaaca
     5761 gaagtgtgca cggcaagatc catcagatga atccccggca tggccagcat catatcggtg
     5821 gtaatatttg taacatcctt tgtgccgcgc attccaaacg agccggacac cttgactccg
     5881 tcatccagat aggagcgata gggtgcaaga gtgtagcgaa gatttccggg aagagatgaa
     5941 tgcaaatccg cataacggac tgttaaatcc ttgatgtgaa attgaatatt ctgaagctcc
     6001 ccgaaactca tatcggttgc ggaattgaga gtcaaaaggt tgacaccaga ttggttctgc
     6061 acggtaagaa gcagcttgtc catggagact gtggtcgtgt ctccggttcc aatctttcca
     6121 tttgctacaa gccccagttc cagaggagca gatctgctgt cctgcatgcg aagccgcgtt
     6181 tccgatacat gcaactcact cttcacgtcc agatcgcgaa aactctccgg cgactgaagc
     6241 cgaatctgcc cggatgcgac tccatccata gagggccccg caaacggagc aagtcgtagg
     6301 cgcgaaacaa tcagatcgac cagtacaccc tctccgggta tgatgtttgc gtttccgcga
     6361 atggtcgcat tgttataaga
//
LOCUS       DAOQJL010000175         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6883_length_6357_cov_4.366868, whole genome shotgun sequence.
ACCESSION   DAOQJL010000175 DAOQJL010000000
VERSION     DAOQJL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_6357_cov_4.366868"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..2187
                     /gene="ftsH"
                     /locus_tag="PLG78_09175"
     CDS             193..2187
                     /gene="ftsH"
                     /locus_tag="PLG78_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNE23327.1"
                     /translation="MNRNARYFILILFGIVVIMMLMGGPSKLLQPLEKPETITFSQFK
                     QMLTPEGRAATGSIFTRASDKEEDSIVLRIAPRKITGRYLKPGVSIEKSDSLDKVRSK
                     TYPFEAEISQGAITEKIQQSLDESGVIYKFENPEEGSLFSSILQILPFVLLIGFLWMF
                     MMRQIQATGNRAMSFGKSKARLMPEGKKKLNFHDVAGCDEAKTELVEVVEFLKDPKKF
                     QAIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRD
                     LFEQAKRQAPCIVFIDEIDAVGRLRGAGLGGGHDEREQTLNQMLVEMDGFEENEGIIV
                     VAATNRPDVLDPALLRPGRFDRQIVVDIPDVKGREAILKIHAKKVPLTSDVSLQNIAR
                     GTPGFTGADLANLINEAALLTARRNKRRVTQDELEEAKDKVMMGPERRSFMITPKEKE
                     VIAYHEGGHALLGTLLAYAEPVHKVTIIPRGRALGLTQQLPTDDKHIHPSRYWEDRIC
                     VLMGGLLAEDIVFGDRSTGASNDIQVATNIARRMVCEWGMSKKIGTINYSTHNDNVFL
                     GREIQSPRTYSDETASTIDEEVNRIVTEQREKGRSLLNQHREKLERIAKALLAHESIS
                     GTELNEIVGADASESQKRSLDPRHPHGMEPASEPSVAPAS"
     gene            2230..3786
                     /locus_tag="PLG78_09180"
     CDS             2230..3786
                     /locus_tag="PLG78_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23328.1"
                     /translation="MDDDLVKLLANPFEEDRLQARAMLAGRAQDLGEGETHDFLRDLR
                     AFRGSPDTVLYTHRSSAETVQQRLFSETLAPGARHAIEPYRARLTQHITSLLKNFKVF
                     VETCGEEILLREIKRLERRYFDTDPTPQQINFLKLKRIYEKIKTFQELARVDWREVFK
                     VVDTLTVIEETRNMARILEKEVLRSIPLLMRRTDHFLESVSSYMGGGEEARDPVTGSY
                     RLAVKYNAELAYTIGHMLGDEIFQEAQEEEKKEETDETEEAETFSGSIARNIVIDTRE
                     KILNKSSLISAPLLGSRDWNRTPAYSMEVPVRQYEECFENFKQSFIVHLEPGVLPANA
                     ASNQMAARLRSGSGESVMDDYVKLVFDLIEEVAQDLVGKDFPGLDKPLIFLYHCGPIA
                     MFNMLLLTMKQRQIGEIQYLDKSGNAIREYPQEMLKKLLIDWWRARFLPLGSEEIDSY
                     INYSRCLDHVKKDYRILYNAGVEVRKKEQPMGLYSGLQRWMRENRARVFGIRRMEVFR
                     RFIPGVFLNF"
     gene            3838..4992
                     /locus_tag="PLG78_09185"
     CDS             3838..4992
                     /locus_tag="PLG78_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE23329.1"
                     /translation="MKQLPRILPFTKEHDDFRAMAADFFDQEVAPHHEQWEKDGIVPR
                     EIWAKAGSLGLICPNFPEEYGGAGADFLYNLIVIEESARVGASGFFISLHADVIAPYV
                     LHHASDEQKQRWLPGIIDGTKILAIAMTEPGAGSDLAGITSTAIDKGDHYLVNGSKTF
                     ISNGYLSDLIITVAKTDPEKEINGISLLMVERGMEGFERGKRLKKIGLNAQDTSELFY
                     HDVKVPKANLLGKKGYGFRYLMSELATERLVLAASSMRGCEVALEETLKYVKTRKAFG
                     KSLGSFQNTQFKLADAATEVAAARCFLDQVILAFMRGDKVVVEASQAKLLCSELLKKT
                     VDTCLQFFGGYGYMMEYPIARAFVDARVQSIYAGTSEIMRGIIASKGLGL"
     gene            5022..5474
                     /locus_tag="PLG78_09190"
     CDS             5022..5474
                     /locus_tag="PLG78_09190"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNE23330.1"
                     /translation="MEEAYELALQADLSGEVPVGALVVELSTLKIVGRGKNEIISRKD
                     PTAHAELLAIREACAHFQSERMLPSVLVSTLEPCTLCTGAILFARVAEVQYFTPVLSG
                     AGIVRLLDQFGTSYNHRPLLTHIESHQEKARQILVSFFERKRARLPEV"
     gene            complement(5475..6281)
                     /locus_tag="PLG78_09195"
     CDS             complement(5475..6281)
                     /locus_tag="PLG78_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23331.1"
                     /translation="MLEAQPVNRTWRDWIRLIPARGADYFLLFYLALDLALILFHDSY
                     RDLLPKGSVTYVRYFDFSLIAIWSIDFILRLRRQEDRWGYVRAHWYEAIGLFPLHPMR
                     YFLALRAAKIGIAYFRILRSDRDVGQLVVEEITFRFRDIFVDTIADAVFLRSLERVDE
                     VMSRLDYETLARSTFQKHQASMQKAVHDSINSKSIIGDLARIPLMGTVVNKIGADVSS
                     IVIEVLETEVTGRIMKDITSSILTEMNERVKLLDLERLTGRRLTTGRTKK"
BASE COUNT         1630 a         1524 c         1658 g         1545 t
ORIGIN      
        1 aggaagccgg tgagccaggt gaaataggcc tcgaatttga accaatggag atcctccagc
       61 tcatcagcat catcaatgat gcggaagctg ccgttcgatc catctcgagc ggggacataa
      121 acgaggtcat cgagaagttc aagcagtgat tgacgtttcc tcgtttcgca cttattatgg
      181 agccgtaata caatgaatcg taacgcgcgt tatttcatcc tcatcctctt tggcattgtg
      241 gtcatcatga tgctcatggg aggcccgagc aaactccttc agcctctgga aaagccggaa
      301 actatcacgt tttcgcagtt caagcaaatg ctgacaccgg agggcagggc agccactggc
      361 tctatcttca cacgggcttc tgacaaagaa gaagacagca ttgttcttcg tatcgcgccg
      421 cgtaaaatca caggtcgtta tctaaagcca ggcgtttcga ttgaaaaatc ggattctctg
      481 gacaaagttc gaagcaaaac ctatccattt gaagctgaga tttctcaggg cgccatcact
      541 gaaaagatcc agcaatccct cgatgagagc ggagtgatct ataagtttga aaatccggag
      601 gagggtagct tgttcagttc cattctccag atcctgccat ttgttttgct catcggtttt
      661 ctctggatgt tcatgatgcg tcagatccag gcaactggaa acagagccat gtctttcgga
      721 aagagcaagg ctcgtctgat gccggaagga aaaaagaagc tgaacttcca tgacgttgcg
      781 ggttgcgacg aagcaaagac tgaacttgtt gaagttgtag aatttctgaa agacccaaag
      841 aagttccagg caattggagc gaagattccc aagggcgttt tgctggttgg tcctccgggt
      901 acaggtaaga ctcttctcgc acgtgctgtt gcaggtgagg ccggagttcc tttcttctct
      961 atttccggat ctgactttgt ggaaatgttt gtaggtgtgg gcgcatctcg cgttcgcgat
     1021 ctgtttgagc aagccaaacg tcaggcgcca tgcattgtat tcatcgacga aattgatgca
     1081 gtaggaaggc ttcgcggagc gggccttggc ggcggacacg atgagcgcga gcagactctc
     1141 aaccagatgc ttgtggaaat ggacggattt gaagagaacg aaggaatcat cgtagtcgct
     1201 gcgaccaacc gtccagacgt actggatcct gctcttcttc gtccggggcg ttttgatcgc
     1261 cagattgtag tagatattcc ggatgtaaaa gggcgcgaag ccattctgaa gatccacgcg
     1321 aagaaagttc ctctcacttc ggatgtgtcg ctgcaaaaca ttgcgcgcgg aactccaggt
     1381 ttcactggcg cggatcttgc caacctcatc aacgaagcgg ctctcctcac cgcgcgtcgt
     1441 aacaagcgtc gcgtgactca ggatgaactg gaagaagcaa aagacaaagt gatgatgggt
     1501 ccggagagaa gatcgttcat gatcactccg aaggaaaaag aagtcattgc atatcacgaa
     1561 ggtggtcacg cacttctggg aactcttctc gcctatgcag agccggttca caaagttacc
     1621 atcatcccgc gcggtagagc tcttggtcta actcagcaac ttcctacaga tgacaaacac
     1681 atccacccga gccgttactg ggaagatcgc atttgcgttc tcatgggtgg tcttcttgca
     1741 gaagacattg tgttcggaga tcgttctact ggagcatcca acgacattca ggtagccaca
     1801 aacattgcac gtcgtatggt ttgcgaatgg ggcatgtcca agaagattgg aaccatcaac
     1861 tattccacac acaacgacaa tgtgttcctt gggcgcgaga ttcagagccc acgcacttac
     1921 agcgatgaaa ctgcttctac cattgatgaa gaagtgaatc gtattgtaac agagcagcgc
     1981 gagaagggca gaagtcttct caaccagcac cgtgaaaaac tggaaaggat tgcaaaagcg
     2041 cttcttgctc atgaaagcat ttctggcacc gagctcaacg aaatcgtggg cgcggatgcg
     2101 tctgaaagcc agaaacgttc tctggatccc agacatccgc atggcatgga acctgcttcc
     2161 gagccttccg tcgctccagc ctcgtaaaaa aggcttgaga cgccctcggg ccggggcgag
     2221 aatcgaagca tggacgacga tttagtcaag ctcctggcca atccattcga ggaagatcgt
     2281 ctccaggctc gtgccatgct cgcgggccgg gcccaggacc tgggggaagg ggaaacccac
     2341 gactttctca gagaccttcg cgcttttcgt ggcagccccg atacagtcct ctatacccac
     2401 cgcagttctg cggaaacagt tcaacagaga ctcttcagcg aaactctagc acctggtgcg
     2461 cgtcatgcga ttgaaccata ccgtgccaga ctgacccagc acatcacaag ccttctgaag
     2521 aacttcaaag tgtttgtgga aacatgcggc gaagaaatct tactgcgcga aatcaagaga
     2581 ctggagcggc gctactttga tacagacccg actccgcaac agatcaactt tctcaaactg
     2641 aaacggatct atgaaaagat caagacgttt caagagctgg cccgtgtgga ctggcgcgaa
     2701 gtcttcaaag tagtagatac tcttacagtg atagaagaga cgcgcaacat ggcgcgcatt
     2761 cttgaaaaag aagtacttcg ttccattccg cttttgatga gacggaccga tcatttcctg
     2821 gaaagtgttt ccagctacat gggcggaggc gaagaagctc gcgaccctgt cacaggaagc
     2881 tatcgtcttg ctgtgaagta caacgccgag cttgcatata cgattggtca tatgctcggc
     2941 gacgaaatct ttcaagaagc acaagaggaa gaaaagaaag aagaaaccga tgaaacagag
     3001 gaggcggaaa cctttagcgg ctccattgcg cgcaacattg tcattgatac gcgcgaaaag
     3061 attctgaaca aatccagttt gatttctgcg ccattgcttg gctcgcgcga ctggaatcgc
     3121 actccagcat atagcatgga agttcctgtc cgtcagtatg aagaatgttt cgagaatttt
     3181 aaacaatcct tcattgtgca tctcgagcca ggagtacttc cggcgaatgc agcttccaat
     3241 cagatggctg cgcgtttgag atctggatcc ggcgaaagcg taatggacga ttacgtgaaa
     3301 cttgtctttg atttgattga agaagtcgcg caggatctgg tgggtaagga ttttcccgga
     3361 ctggacaagc cactcatctt tctctatcat tgtgggccga tcgccatgtt taacatgctt
     3421 cttctgacca tgaagcagag acagattgga gagattcaat atctggataa gtccggaaac
     3481 gcaattcgag agtatcctca ggaaatgctc aagaagctat tgatagactg gtggcgcgcg
     3541 cgatttcttc cgctcggttc tgaggagatt gattcctaca tcaactattc acgctgtctc
     3601 gatcatgtga agaaagacta tagaattcta tacaatgcgg gtgtagaggt tcgaaagaag
     3661 gaacagccga tggggctcta ctctggtttg cagagatgga tgcgcgaaaa tcgcgccaga
     3721 gtgtttggaa ttcggcgcat ggaagttttc cgtcgtttta ttccgggcgt gtttctcaat
     3781 ttttgattgc gacagcgtgg ttttttctga ctcttggtct accggccgga ggccaacatg
     3841 aaacaacttc cacgcattct tcctttcacg aaagagcatg atgactttcg cgcgatggct
     3901 gcggatttct tcgatcaaga agtagctccg caccatgaac aatgggagaa agacggcatt
     3961 gttccgcgtg aaatctgggc caaggcaggc tctcttggtt tgatctgtcc caatttcccc
     4021 gaagaatatg gtggagcggg cgctgacttt ctctataatc tgattgtaat tgaggaatcc
     4081 gcgagagtgg gagcgagcgg tttcttcatc agtctgcacg cagacgtaat tgcaccgtat
     4141 gttttgcacc acgcaagcga tgagcaaaaa cagcgctggc tgccagggat cattgacgga
     4201 acaaagattc tggccattgc tatgacggaa cctggtgccg ggtcggatct cgcgggcatt
     4261 accagtactg ccatagacaa aggggatcat tatctggtaa acggatccaa aactttcatt
     4321 tccaacggat atctttccga tttgatcatc actgttgcca aaacagatcc tgaaaaagag
     4381 atcaacggaa tttcccttct catggtagaa cgcggaatgg agggatttga gagaggcaag
     4441 cgtctgaaga agattggcct gaatgcgcag gatacttctg aattgttcta tcacgatgtt
     4501 aaagttccta aagcaaatct tctgggaaag aaaggatatg gttttcgcta tttgatgagc
     4561 gagcttgcaa cagaacgatt ggttctcgcg gcgtccagta tgcgcggctg cgaagtggct
     4621 ctcgaagaaa ctctcaaata cgtgaaaacc cgaaaagcgt ttgggaaaag tctgggcagc
     4681 tttcagaaca ctcagttcaa actggcagat gctgccacag aagtggcggc cgcgcgttgt
     4741 tttctcgatc aggtgatcct ggcgttcatg cgaggcgaca aggtagtcgt agaggcttcg
     4801 caggcaaaac tactgtgctc agaacttctc aaaaagacgg tggatacgtg ccttcagttc
     4861 ttcggaggct acggctacat gatggaatat cccatcgcac gcgcctttgt agacgcgcgc
     4921 gttcagagca tttacgctgg aacatcagag atcatgcgcg ggatcattgc atccaagggt
     4981 ctgggtctct gacgagttca catccgcttc ctgctgaaat cctggaggaa gcatacgaac
     5041 tggcccttca ggcggatcta tcgggcgaag ttccagtcgg agctctggtt gttgaactct
     5101 ccactctgaa gattgtgggg cgcggcaaga atgaaatcat ttcgcgcaaa gatccaacgg
     5161 cccacgcaga gctcctggct attcgcgaag catgcgcgca ttttcagtct gaaagaatgc
     5221 ttccgtctgt tcttgtttct actcttgaac cctgcaccct atgcaccggt gcgattctgt
     5281 ttgcgcgcgt tgcggaggtt caatatttta cgcctgtgct ctcaggggcg ggtattgtgc
     5341 gattgctcga tcaatttggt acttcttaca atcacagacc acttctcact cacatagaaa
     5401 gccaccagga aaaagccagg cagattcttg tttccttttt cgagagaaag cgagcgcgtt
     5461 taccggaagt ctgattactt ttttgtgcga cctgtggtca gtcttctgcc ggtgagtcgt
     5521 tctagatcga gcagcttcac cctctcattc atctcagtta gaatgctaga agtgatatcc
     5581 ttcatgattc gtccagtgac ttccgtttcc agtacctcga tgacaatgga gctaacgtct
     5641 gctccgattt tgtttacgac tgtacccatc agaggaatac gagccaggtc tccgatgatt
     5701 gacttcgaat tgatggagtc gtgaactgct ttttgcatgg acgcctgatg cttctgaaac
     5761 gtggatcgag ccagtgtttc atagtccagc ctgctcatca cttcatccac tctctcgagt
     5821 gatctgagga acacagcatc ggcgattgta tccacaaata tatcgcggaa tcggaatgtg
     5881 atctcttcta ctacgagttg ccctacatcg cggtcagacc tgaggattct gaaatacgca
     5941 atgccgatct tggccgcgcg aagagccaga aagtatcgca ttggatgcag cggaaaaagt
     6001 ccaatcgctt cataccagtg tgcgcgcaca tatccccatc gatcttcctg tctgcgaagg
     6061 cgaaggatga agtcgatgga ccagattgct atgagggaaa agtcaaagta gcgaacgtaa
     6121 gtgacactgc ctttgggaag gaggtcgcgg tatgaatcgt gaaacaagat gagtgccaga
     6181 tccagtgcca gatagaagag caggaagtag tctgcccctc tggcgggaat caatcgaatc
     6241 cagtcacgcc aggtgcgatt tacgggctgc gcttcaagca ttaaactgct gcaactggag
     6301 caagcgccag gtccggtgtc atgcgctgca aaagagtttc tgcacgcttt ctatggg
//
LOCUS       DAOQJL010000176         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6884_length_6357_cov_3.316090, whole genome shotgun sequence.
ACCESSION   DAOQJL010000176 DAOQJL010000000
VERSION     DAOQJL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6884_length_6357_cov_3.316090"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLG78_09200"
     CDS             <1..378
                     /locus_tag="PLG78_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNE23332.1"
                     /translation="STPTIAQSWRGIEMKKMSKWFLTFLPLLLLSACISSPLQGWLIT
                     YTGSHSSMVDKGAISSAKIVKKGESCSWSAWIFKDIYYGGGGDVKEAADSANIKKIAV
                     VDRRSFSLLFDLVFKECVLVYGE"
     gene            381..1178
                     /locus_tag="PLG78_09205"
     CDS             381..1178
                     /locus_tag="PLG78_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23333.1"
                     /translation="MSLQWRTFIIAAALLAASRVHAQSDELYRKDRLYVDIAYGTGPT
                     TVDFVAAGFGNASYTAALVDLSLGNQNNRNLALLYFLTKAGKNPNVSMSGSQRIGIEY
                     AATSWLTFGVGASHFTGTISGGGLNQLDGLGNLGYLIMLYNYTSVGSSSGNSLSSTLY
                     TLPYSVSINTSIAEIQYAVHVQAGFADPYFRMSGGSAKGISKSEIAFGSRFRFAGNAY
                     VYTEAFYTQYALNSDLTQSTIAATVVNKGARFGVGYAFDPPDTIASK"
     gene            complement(1206..2219)
                     /locus_tag="PLG78_09210"
     CDS             complement(1206..2219)
                     /locus_tag="PLG78_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23334.1"
                     /translation="MSLWKISLAFLFVFFSCGKPPVQPLDHCQKINGMPGPEDFDYDR
                     AGNRLLVSSQERRKQNGGYPEGAIFAVDLSGKEAVGTARALPITGRDSSPFHPHGISL
                     FAGKSETLLYVVAHPDEEHNAIEVFRVLPDSLAFKERLTSEVLVHPNDVASLANGELY
                     VTNDHGWTGGLAVLEDLFALPRSTVAHYREGKWSFVADGFRVANGIAVNRALDRLYVG
                     ATRDRGIHVYVRNPADGSIGKELDFIDMGSGTDNVMWESDEILDVAGHYSMIRFIRTV
                     KDPTALSPNDITRVNVTSGKKEMIFATDGSEISGASTGLVLKGKLYISQVFEPFILSC
                     DMK"
     gene            complement(2265..2393)
                     /locus_tag="PLG78_09215"
     CDS             complement(2265..2393)
                     /locus_tag="PLG78_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23335.1"
                     /translation="MMHHKRIEEDRTIKKEFARVPLSRGLHECIPSTRVFSRNYIA"
     gene            2555..2914
                     /locus_tag="PLG78_09220"
     CDS             2555..2914
                     /locus_tag="PLG78_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23336.1"
                     /translation="MCLHSVYSTLQHIPGSAAQHILSLGLARLANSLPSWLRTGGEVF
                     PAIFIGSLFIVWNEYLISFLRATDIGSAGVGAVITTLCFNGFVPCRIALLATPRLRPV
                     NVAVGIIALITYIAGLA"
     gene            2931..3266
                     /locus_tag="PLG78_09225"
     CDS             2931..3266
                     /locus_tag="PLG78_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNE23337.1"
                     /translation="MNIQIFGLKSCPNTRKAEMFFKERRITYQFINLAEKPMSRGELT
                     SVMASIPAEELIDTESRAYVSAGLQYMKFNIAEKLLEEPRLFKTPIVRDAKRATAGFA
                     PEIWKKWLS"
     gene            3443..4687
                     /locus_tag="PLG78_09230"
     CDS             3443..4687
                     /locus_tag="PLG78_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE23338.1"
                     /translation="MQIDSGTTAWMLIATALVLLMVPGLAMFYGGLVRTKNVVGTMMH
                     SFVAMAVIGTLWVAVGYSLTFGKSIAGGIVGWNWDYFFLKGIDESVVNHVPEYVLAMF
                     QGKFAIITPALISGAMAERVSFRGYLAFIVLWFLIVYCPLAHWIWAADGWLATTGPLG
                     VIDLAGGLVIHVSAGTSALVVALFLGKRYGFPHNTQNPNNLVMTLMGAGLLWVGWFGF
                     NAGSTVQSGLDTARALTMTQISAAAGASTWMVIEAIKFRKVTSLGFVSGILAGLVVIT
                     PAAGVVLPSGALILGMCSSVLCFFALEMKNRLGYDDSLDCFGIHGVGSGMGVLALSFL
                     IRDSWLAKAAGIAGSWDHLAQFVVQLKGFGATVALSAVATIAICFVVEKTIGLRVSKS
                     EELAGLDHTEHRESGYGLINLN"
     gene            4711..5052
                     /locus_tag="PLG78_09235"
     CDS             4711..5052
                     /locus_tag="PLG78_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNE23339.1"
                     /translation="MKMIIAVIQPDKVDEVREALIAADITRISINRITGHGQQADTEL
                     FRGQEVTPNLLSKVEIKIACNDQFVDIIVQTIMKAARHGEGAIGDGKIFVLPLERCYR
                     IRTGESGNGAI"
     gene            5103..5963
                     /locus_tag="PLG78_09240"
     CDS             5103..5963
                     /locus_tag="PLG78_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE23340.1"
                     /translation="MSQSDIVEYYSEAGQDYSAWSPAFNMHFGYYRKWMNPFRLEPML
                     EQMNAEILSRMTGGKPAREMEFLDAGCGLGATARHIARQASAKVLGITIVPWQIEEAG
                     KLAARAGLSVRITFDLRDYRDTKLPPNQFDGVYALESSCYAGAGDKADFIRESFRLLK
                     PGGRLVVADGFLSRLNSKPNAIYRKCLSLMEEFWALETFAHKEPFLAALEKAGYRDVQ
                     IENANFRIAPSVAFIPWVTAKFFIKDVIAKRRTLTRRQLGNALAPILGLIVGLHMHRF
                     SYCIVTARRP"
     gene            6009..>6357
                     /locus_tag="PLG78_09245"
     CDS             6009..>6357
                     /locus_tag="PLG78_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23341.1"
                     /translation="MQSQSSPISRTGPKWIMAAAAAGLLLVGGLVFYFVDFFPSLSHV
                     NVRLYSGATTGDYYAGAARSVQIAQSHQGKIENISTSGSIDNLKRLEQEREHGRFALV
                     QNGMPWPPGLQLVG"
BASE COUNT         1530 a         1633 c         1651 g         1543 t
ORIGIN      
        1 tctactccaa ctattgcaca atcgtggcgg gggattgaaa tgaaaaaaat gagcaaatgg
       61 tttcttacat ttctacctct gctgctgctg agcgcttgca tcagttctcc tctgcaaggc
      121 tggctgatca catacactgg aagtcactcc agcatggtgg acaaaggtgc gatctcttct
      181 gcaaagattg tgaagaaggg agagtcctgt tcctggtctg cgtggatctt taaggacatc
      241 tactacggtg gtggtggaga cgtgaaagag gcagcggatt ctgcaaacat caagaaaatt
      301 gcggtggtgg atcgcagatc attctcactg ctgttcgatc ttgtcttcaa agaatgtgtc
      361 ctggtctacg gagaatgatc atgtctctgc aatggcggac attcatcatc gcggcggcgc
      421 tccttgcggc gtcccgggtg cacgcgcaat cggatgagct ctaccggaaa gacaggctct
      481 acgtcgacat tgcgtatgga accgggccta ctacagtaga tttcgttgca gcggggttcg
      541 gcaacgcaag ctacacggct gcactcgtag atctttcgct gggaaaccaa aacaatcgta
      601 acctggccct gctctatttt ttgactaagg cgggaaagaa tccgaatgtg tccatgagcg
      661 gaagccagag aattggcatc gaatacgcgg ctacgagctg gctaacattc ggcgtcgggg
      721 caagccactt caccggaact atttcggggg gcggtctcaa ccaactggac ggcctcggaa
      781 acctgggtta tctgatcatg ctgtataact atacgagcgt gggctccagt tccggaaata
      841 gcctgtcctc cacgctctac actctgccgt attctgtttc gatcaatacg agcatcgcgg
      901 aaattcagta tgcagtgcat gttcaggccg gctttgcaga tccttacttt cgcatgtccg
      961 gtggatctgc gaagggaatc tcaaagtcag agatagcatt tggctccaga ttccgttttg
     1021 cagggaatgc gtacgtgtat acggaagctt tctatacgca gtatgcgttg aattcggacc
     1081 tgactcagag cacaattgcg gccacagttg taaacaaagg cgctcggttc ggggtgggtt
     1141 atgcgttcga tccgccagat acgattgcct ctaaataatt gccgcaaaca ggaaagaaga
     1201 gccgctcatt tcatgtcgca ggatagaatg aacggctcaa atacttgaga aatatagagt
     1261 tttcctttca atacgagtcc cgtggacgca ccactgatct ctgaaccgtc ggttgcaaaa
     1321 atcatttctt tctttccgga tgtaacgttc actcgagtga tatcgttggg agaaagtgca
     1381 gttgggtctt tcacagttcg gatgaatcga atcatgctgt aatgccctgc cacgtctaaa
     1441 atttcatccg actcccacat cacgttgtct gtccctgaac ccatgtcgat gaaatccagt
     1501 tcctttccaa tggacccatc tgccggattg cgaacataca catgaatgcc tctgtctctg
     1561 gtggcgccta cgtacaaccg gtcgagtgca cgattcaccg caattccatt tgcaacgcga
     1621 aagccatctg cgacaaaact ccacttccct tcgcgatagt gagcgaccgt tgatcgaggg
     1681 agggcaaata agtcttcaag aacagccagt cctcccgtcc agccatgatc attggtaacg
     1741 tatagctctc cgtttgcaag actggccaca tcgtttggat gaaccaggac ttcagacgtt
     1801 aggcgctctt taaaggcaag agaatctgga agaacacgaa acacttcgat cgcattgtgt
     1861 tcttcatccg gatgagccac aacatacagc agcgtttcgc tttttcccgc gaacaatgaa
     1921 attccatgag gatgaaatgg ggacgaatct cttcccgtaa tcggcagagc acgggctgtt
     1981 ccgactgctt ctttcccgga aagatccacg gcaaagatgg caccttccgg ataaccgcca
     2041 ttctgttttc ttctttcctg agaggataca agaaggcgat tgcctgcccg gtcataatca
     2101 aagtcttccg ggccgggcat tccgttgatc ttctggcagt gatccagagg ctgaacgggt
     2161 ggcttaccgc atgaaaaaaa tacaaacaaa aaggccagag aaatcttcca gagtgacatg
     2221 gcccaggctg caggcccgga acttcgcggg caactctttt aacgttatgc gatgtagttg
     2281 cgcgaaaata cacgtgtaga aggaatgcat tcatgcaggc cgcgcgaaag tggaacacgg
     2341 gcgaactctt ttttgatggt acggtcctct tctattctct tgtggtgcat catgccttac
     2401 tcagaggcag acactttgca gaattccttt ctccagccgg ggcattctat gcttacctgg
     2461 cgctcagttt ttttctgccc tggttcttag gctccatcta caggagattt gcagactccc
     2521 cccgcgccct tcgtctggcc gccctcggca gcggatgtgc cttcattctg tttactctac
     2581 tttacaacac attccaggga gtgcagcaca acacattctc agtcttggcc tggcgcgcct
     2641 cgcaaacagc cttccttcct ggctcaggac gggaggagaa gtgtttcccg cgatcttcat
     2701 tggcagttta ttcattgtct ggaacgaata cttgatttcg tttttgcgag cgacagacat
     2761 tggatcggcg ggagtcggtg ctgtcatcac aacactttgt ttcaatgggt ttgtgccttg
     2821 cagaatcgca ttgctcgcga caccgcgatt acgaccagtg aatgttgcgg taggaatcat
     2881 tgccctcatc acatacatcg cgggacttgc gtaaagagca ggagagaaag gtgaacatac
     2941 aaattttcgg actcaaaagt tgccctaaca ctagaaaggc ggaaatgttc ttcaaggaac
     3001 gccgcataac gtatcaattc atcaacctcg cggaaaagcc aatgtccaga ggagagctca
     3061 cttccgtgat ggcatccata ccggcagaag agctcataga cactgaatcc agggcgtatg
     3121 tttctgcggg acttcagtat atgaaattca acattgcaga aaagctactg gaagaaccac
     3181 ggctcttcaa aactcccatc gtccgcgatg cgaaacgtgc cacggcggga tttgcgcctg
     3241 aaatctggaa gaagtggctc tcttgatcca gagaatcagc gcctgaggca ttttgccgca
     3301 taagagcgca gcatgcatcc agtctaatca gattgcctct ttttggattg cgtccttcgc
     3361 gcaagataca aatttgccgg cgagcgttcc gaactggcgc aagcacagcc acagcacgcc
     3421 tcactctatt ggaaggaagt ttatgcagat cgattctgga actactgcct ggatgctcat
     3481 cgccacggcc ctcgtgctgc tgatggtacc gggcctggcc atgttttacg gaggcctggt
     3541 tcggaccaag aatgtggtag gcacaatgat gcacagcttc gtggccatgg ctgtcatcgg
     3601 cactctctgg gttgcggttg gctattcgct cacatttgga aagagcatag cgggcggaat
     3661 tgtcggatgg aactgggact acttcttcct gaaaggaatt gatgaatcgg tagtgaatca
     3721 cgttccagaa tatgttctgg ccatgttcca gggaaaattt gcaatcatca ctcctgctct
     3781 catcagcggt gcgatggcgg agagagtttc cttccgcggc tacctggcat tcatcgtatt
     3841 gtggtttctg attgtctact gtcccctggc ccattggatc tgggctgcag acggctggct
     3901 ggctacaaca ggcccgttag gcgtcattga tctggccggc ggcctggtca ttcatgtatc
     3961 tgctggaacg agcgcactgg ttgtggcact ctttcttgga aagagatacg gcttcccgca
     4021 caacacgcaa aatccaaaca acctggtgat gactctgatg ggtgcgggtc tcctgtgggt
     4081 aggctggttt ggattcaatg caggatccac agtgcaaagc ggcctggata cggcgcgcgc
     4141 ccttaccatg acgcaaatat cagcggccgc aggtgcaagc acctggatgg tgatcgaagc
     4201 aattaaattt cgcaaggtta cttctcttgg atttgtatcc ggaatcctcg cagggctggt
     4261 ggtgatcact cctgctgcag gtgtagttct cccttcgggt gcactgattc tcgggatgtg
     4321 ctcgagcgta ctctgtttct tcgcactcga aatgaaaaac cgtctggggt acgatgatag
     4381 cctggactgc tttggaatcc acggagtggg cagcggcatg ggagtacttg ctctttcatt
     4441 cttgattcgc gactcatggc ttgcaaaagc tgccggaatt gcaggatcgt gggatcatct
     4501 agcacagttc gtagttcaac tgaaaggatt tggagcaacc gtcgctcttt cagccgtcgc
     4561 gacgatcgcc atctgctttg ttgtagaaaa aacgatcgga ttgagagtct ccaagagcga
     4621 agagcttgca ggccttgacc acacagaaca ccgcgaaagc ggctacggcc ttatcaacct
     4681 caactaacac ggattaaacg gaattcgcaa atgaaaatga tcatcgcagt tatccagcca
     4741 gacaaagtag acgaagtgag ggaagctctc attgcagcag acatcacgcg catctccatc
     4801 aatcgaatca cgggccacgg gcagcaggca gacacagaac tcttccgcgg ccaggaagta
     4861 acccccaacc ttctctcgaa ggtggaaatc aaaatcgcat gcaatgacca gtttgtagac
     4921 atcattgtgc agaccatcat gaaagcagcc cgtcacggtg aaggtgcgat tggagacgga
     4981 aagatcttcg tcctgcctct cgagcgctgc tatcgcattc gcaccggcga aagcggaaat
     5041 ggtgccatct aaccccgcgt aatcgttctt gaaggtctgc aggcaacccg cacacgtggc
     5101 gggtgagcca gtcggacatc gtcgaatact attccgaagc cggccaggac tacagcgcct
     5161 ggagcccggc gttcaatatg cacttcgggt attaccgcaa gtggatgaat ccgtttcgac
     5221 tcgagccaat gctggagcag atgaacgctg agatcctgag caggatgacc ggcggcaaac
     5281 cagcacgtga aatggaattt ctggacgcag gatgtggcct cggagccact gccagacaca
     5341 ttgcacgcca ggccagcgcg aaagtccttg gaattacgat tgttccctgg caaattgagg
     5401 aagcgggtaa gctggcagca cgtgcaggtc tttctgttcg cataacgttc gatttaagag
     5461 actatcggga tacaaagctc ccccccaatc agttcgacgg cgtctacgct cttgaatcct
     5521 cctgctatgc aggagcagga gacaaagcgg atttcattcg cgaatcgttc aggctactga
     5581 agccaggcgg acgattggtc gtggcagacg gtttcctatc gcgactgaac tcgaaaccaa
     5641 acgcaattta tagaaagtgt ctttccctca tggaagaatt ctgggcactt gaaacctttg
     5701 ctcacaagga gcctttcctt gcagctcttg agaaagcagg atatcgcgac gttcaaattg
     5761 aaaatgcgaa ttttcgcatc gcgccgtctg tagcattcat cccgtgggtg actgcgaagt
     5821 tctttatcaa agatgtgatt gcgaagagaa gaactctcac tcgtagacag ctcgggaatg
     5881 ctctggcgcc gattcttggt ctgatcgtgg gactacacat gcaccgattt tcctattgca
     5941 tcgtaaccgc ccgccgcccc tgattttttc gattgattgc acaatgcact cttcatgatg
     6001 gctttgcaat gcaaagtcaa tcctcaccca tttcgcgcac aggaccaaaa tggatcatgg
     6061 cggctgccgc cgcgggactc ctccttgtcg gagggcttgt gttttacttt gtggatttct
     6121 ttcccagcct gtctcatgta aatgttcgcc tgtattccgg cgccacgact ggagattact
     6181 acgcaggcgc agcgcgatct gttcaaatcg cacagtctca ccagggcaaa atcgaaaaca
     6241 tatccacaag cggatccata gacaatctca agcgactcga gcaagagcgc gaacacggtc
     6301 gattcgctct ggtgcaaaat ggaatgccgt ggccgccggg gctgcagctg gtggggc
//
LOCUS       DAOQJL010000177         6345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6904_length_6345_cov_3.799841, whole genome shotgun sequence.
ACCESSION   DAOQJL010000177 DAOQJL010000000
VERSION     DAOQJL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_6345_cov_3.799841"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLG78_09250"
     CDS             <1..662
                     /locus_tag="PLG78_09250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE23342.1"
                     /translation="LFYAPGLSVALDLNRMRYSEKLILHPAPIGTFGLLGADLALEFG
                     RDIEPWGPFLSYRVGRSPPAMADSMFVIEPLERSAGTEHLEIIRSDPSVQCFGCGGAN
                     AAGLALPFIFDPRANTARSWFKPPAWMAGHPSWMHGGFISLLLDEVCAKVLRGLQRKG
                     ATVELNVAFRKPVPLGHEIELLAKLETIEGRRIRVQGSILAEGHVRAEAQALFVELLS
                     A"
     gene            complement(629..1291)
                     /gene="tmk"
                     /locus_tag="PLG78_09255"
     CDS             complement(629..1291)
                     /gene="tmk"
                     /locus_tag="PLG78_09255"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNE23343.1"
                     /translation="MPCSIPQITPVPGLFVVVEGIDGSGKSTLVTGLGKALVDHAVLS
                     LCEPTKLASGLLIRRHLTDHSPLPQEAWLEMFGADRRIDLETNINPALARGEIVLLDR
                     YLYSTAAYQGSAGKLNAKEILHSQTNQFRQPDVLIYLDISPEDALARVSATREKLEIF
                     ETSHELHRIHSNYEEILQEVAQEQAGPTLIRIAATDSLDMNIAKAEKAIRQALSNSTK
                     RA"
     gene            1354..2664
                     /locus_tag="PLG78_09260"
     CDS             1354..2664
                     /locus_tag="PLG78_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23344.1"
                     /translation="MRLMGRIYQVIFCLVGLANFALVLFDATYLWKLPYTRLTARDLY
                     LAHAPDLVRRYDPVKGIDAHRFTTAYLAQADHAFELSQAGNYKDAEKVYADLAAMSRE
                     VIDQRPGFSHFSIAEKDGTLQVIKNAMRGHFGIESAKDSFARYWSRENLALDRIAAEK
                     GFFDREIRPLMAQNYFRWIDEDGEMRDYFYRIDLWFVAFFLVDFLARWVIAIRLGRHR
                     KWYMFPVRHGVEIFNLVPPHHAVWMRLLRAIPLYLRMKKAGMIPGEGIMPEILHDNAA
                     LIAEEISGLVLVNILDQLAVMVRDANPSGIVQSEVAQVTKDLLDSQLDRIADTVVPDV
                     EPQITGLVIHSVYQAMGPYLRTPLGPAIRLGMYAVEAAIKDGLRSALVSQDGREQMKG
                     ILRKFLSEGTTELTASENLAVIQNQSSILLDRIKHEVLRSLDEG"
     gene            2709..4058
                     /locus_tag="PLG78_09265"
     CDS             2709..4058
                     /locus_tag="PLG78_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNE23345.1"
                     /translation="MQMDLPSKPYYVRLPDLDFFDLFLKIDQAYEHCFLLESLGEWTA
                     DSRYSVIGFDPDQTLRGTPDGLFVGNQLIPSENPYRLLKEFLPAGIISRNYCGGLVGY
                     IGYDATKFFEPSLDLTPHPDFDSFVFGVFTDGLIHDSMTGETFYFYYRKDRSSQILEL
                     LGSGNRSLSARVVSLGDAVDQATHAAMVAETKEEILRGNTFQCQIGFRSDFELKGDAL
                     AVYRILRTVNPSPHMFYLKADGRTLLGASPELAFRLRQGEMETFPLAGTTRRGSFDEE
                     DRRLARGLLNDPKEIAEHNMLVDLHRNDLGRVARPGTVKVRRLMDIKRFSHVQHISSE
                     VVGIISRQHDMFSGLASVFPAGTLSGAPKIESMKIIERIEADPRGPYGGAVGHFGFNG
                     DCTFAIPIRSLFLRGDRGFLRASGGIVFDSTPEGEYAEIQAKLASMRKALAPFMVQV"
     gene            4055..4669
                     /locus_tag="PLG78_09270"
     CDS             4055..4669
                     /locus_tag="PLG78_09270"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNE23346.1"
                     /translation="MKVLIIDNYDSFTYNLFQIAAEALEESSSQLDVVRNDELTEEEI
                     RRRNYDRIILSPGPGSPDDPAYFGVCAAVLTSIGQVVPILGVCLGMQGLAHYYGGKVV
                     RAQIPMHGKVSPIEHDSRGVFADLPQGLSAMRYHSLVVEEASLPDCFELTARAVDGAR
                     ELMGIRHKTLPLEGIQFHPESFATEGGIRMIENFLFRPERSGST"
     gene            complement(5168..>6345)
                     /locus_tag="PLG78_09275"
     CDS             complement(5168..>6345)
                     /locus_tag="PLG78_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE23347.1"
                     /translation="YSACMAHPIYRTDSMPRNDSTYLRNLLSRPEFLPFQRILQTPDQ
                     HRLQILVTEFQRDGRTLKPILHSYRLREQEYFYPASLVKLPVALLALEKVQKIGIPGI
                     NLDTSYRLEPGDCQQEDLGPSTIRRDILAMFAVSENEAFDRLYSFLGQDSIVAGLRMR
                     GYKTGQVTHRLAAKCSMEENRHYNGVDFESPEGETLLRQEPRVTTEHYTNHLEGMDIV
                     HGYDGTERSFLSIPELHRLTTAAVLPELLPSSATFALNDESRTLLLKAMSTMPEDTDL
                     DFDEQDHQGCTSKYLVMGGSRERLPKKFSIFNKVGKALGFLTDSALLIDRESKRTLLV
                     TVTLYVNYARSANDEDHHYKDLGLPFLATLGQQLLRSQNERQDQPLEVEEFFNLYQR"
BASE COUNT         1359 a         1579 c         1730 g         1677 t
ORIGIN      
        1 gtctcttcta tgcgcccggt ctttctgttg cactcgatct aaataggatg cgatattctg
       61 agaagctgat ccttcatcct gcgccgattg gcacctttgg gttgttaggc gctgatcttg
      121 cgctcgaatt tggccgggat atcgaaccat ggggtccgtt tctgtcctat cgcgttggac
      181 ggtcgcctcc tgccatggca gattcaatgt ttgtgatcga gcctctggag cgatcggcag
      241 ggaccgaaca cctcgagatc attcggtcag atccttcggt tcaatgcttt ggatgcggcg
      301 gtgcaaacgc agctggattg gcgttgcctt tcatctttga tccgcgagcc aacactgcgc
      361 gaagctggtt taagcctcca gcctggatgg ccgggcatcc gtcctggatg cacggcggtt
      421 tcataagtct actgctggat gaggtttgcg cgaaggtgct tcgcggattg cagagaaagg
      481 gagcgacggt cgagctcaat gttgcgttcc gtaaacccgt gccgctgggg catgagattg
      541 aactgctggc caaactcgaa accatagaag gtagaaggat tcgggtgcag ggttccatcc
      601 tcgccgaagg gcatgtgcga gcagaagctc aggctctttt cgtggagttg ctcagcgcct
      661 gacggattgc tttttctgct ttggcgatat tcatatccag cgagtctgtt gcggcgattc
      721 taatcaacgt gggacccgcc tgctcttgtg ctacttcctg taggatctcc tcatagttcg
      781 agtggatcct gtgcaattcg tggctggttt caaatatttc gagtttttct ctggtagcgc
      841 tcactcgggc cagggcatct tcgggcgata tatcgagata gatgaggacg tccggctgtc
      901 ggaattgatt cgtctgggaa tgtaggatct ccttcgcgtt cagttttccg gcggatccct
      961 ggtaggccgc tgttgagtaa agataccgat ccaggagaac gatctcgcct ctagccagag
     1021 caggattgat attcgtttcc aggtcgatcc ttctgtctgc gccgaacatt tcgagccacg
     1081 cctcctgggg cagcggtgaa tggtccgtta gatgcctgcg tatgagcaac ccggatgcga
     1141 gtttcgtggg ctcgcatagc gaaaggaccg catgatccac cagggctttc ccgagtccag
     1201 ttacaagagt gctctttccc gatccgtcaa tcccctctac gactacaaac aaccctggca
     1261 caggagtgat ttgtggaata gagcagggca ctcaagcgtt tttggcggaa agtgtttgac
     1321 tgcagatcgt tccgcaccga cgaacagaaa gtcatgagac ttatgggacg catttatcag
     1381 gtgattttct gccttgtagg cctggccaat ttcgcgctgg ttttgtttga tgctacctat
     1441 ttgtggaaac ttccctatac tcgtttgact gctcgcgacc tgtacctggc tcacgccccc
     1501 gatcttgttc gacgttatga tccggtgaaa ggaatcgatg cgcatcgttt tacaaccgct
     1561 tacctggccc aggcggatca cgccttcgag ttgtcccagg ccggaaatta caaagatgca
     1621 gaaaaggtgt acgccgacct ggcggccatg agccgcgaag tgattgatca gcgtcccgga
     1681 ttcagtcact tcagcattgc ggaaaaagat ggtactttgc aagtaatcaa gaacgcgatg
     1741 cgcggtcact ttggcattga gtctgccaag gactcatttg cgcgctactg gtccagggag
     1801 aatctggcac tcgatcggat tgcagccgag aaagggttct tcgacagaga aattcgaccc
     1861 ttgatggccc agaactattt cagatggatc gacgaagacg gtgagatgcg cgattacttc
     1921 tataggatcg atctctggtt tgtggcattc ttcctggtcg attttctggc gcgctgggtg
     1981 attgcgattc gcctgggtcg tcatcgcaaa tggtacatgt tcccggttcg ccatggcgtt
     2041 gagattttca atctggttcc tccccatcat gcagtgtgga tgaggctcct tcgtgccatt
     2101 ccattgtatt tgagaatgaa aaaagccggg atgataccgg gcgaaggcat catgccggag
     2161 atcctacatg acaacgccgc cttgatcgca gaggagattt ccgggctcgt tcttgtaaat
     2221 atccttgatc agctggctgt tatggttcga gatgcaaacc cctcaggaat cgtccagagc
     2281 gaggttgctc aggtgacgaa agaccttctg gattctcaac ttgaccgcat agcggacacg
     2341 gtcgtccccg atgttgagcc ccagattacc ggccttgtga ttcattccgt gtatcaggct
     2401 atggggccct atttgcgtac gcctcttggc cctgcgattc gactgggcat gtatgcggtg
     2461 gaagccgcca tcaaggacgg tttgcggtcg gctctggtga gccaggatgg tcgcgagcaa
     2521 atgaaaggta tccttcgtaa attcttgagc gaggggacga ccgaattgac cgcctcagaa
     2581 aacctcgctg tgattcaaaa ccagtcttcc attcttctgg atcgaatcaa gcatgaggtc
     2641 ctacgcagcc ttgacgaagg gtgattttgg attttcgata gcgcgggtta tcgggcaatg
     2701 gccattcaat gcagatggac cttccctcga agccgtacta tgtgcgactg ccggatcttg
     2761 acttctttga tctttttctc aagattgatc aggcctacga gcattgcttc ttactagagt
     2821 cattggggga atggaccgct gattcacgtt actcggtgat agggtttgac ccggaccaga
     2881 cgctacgggg tacgcccgac ggtctgtttg tgggaaacca gctgattccg tcggagaacc
     2941 cataccgatt gttgaaggag ttcctaccgg ccggcattat ttcgcgcaat tactgcgggg
     3001 gcctggttgg ctacatcggc tacgatgcaa caaagttttt cgagccttct cttgatttaa
     3061 ccccacatcc agattttgat tctttcgttt tcggcgtctt tacagacggg ctcatacatg
     3121 actcgatgac gggtgagaca ttctatttct actaccgtaa agatcgctct tcgcagattc
     3181 ttgagctact tggttctggc aaccgctccc tttcggcacg ggtagtgtct ctcggagacg
     3241 cggttgatca agcgacgcat gccgcaatgg ttgccgagac caaggaggag atcctacgcg
     3301 ggaatacctt tcaatgtcag attggcttcc gatcggactt tgaattgaaa ggggatgcac
     3361 tggctgttta ccgcatcctc cgaaccgtca atccttcccc acacatgttc tatctcaaag
     3421 ctgatgggag gactttgctt ggagcgagtc ccgagcttgc gttccggctg cgtcagggag
     3481 aaatggaaac atttcctctg gcaggaacca cgcggcgggg atcctttgac gaagaggacc
     3541 gtcggctcgc tcgcggactc ttgaatgatc caaaagaaat cgcagaacac aacatgcttg
     3601 tggatttgca tagaaacgat ctgggtcgcg tagctcgccc cgggaccgtt aaagtgcggc
     3661 gcctaatgga tattaaacgt ttcagtcatg tgcagcatat ttcgagtgag gtggtcggaa
     3721 tcatctcgcg tcagcatgat atgttctcgg gacttgcttc agtctttccc gctggaacgc
     3781 tgtctggtgc tccgaaaatc gaatctatga aaatcataga gcggatcgaa gcggatcctc
     3841 gcggaccgta cggaggagcg gttggacact ttggattcaa cggtgactgt acatttgcaa
     3901 ttcctattcg ttcccttttc cttcgcggcg atcggggttt tctccgggct tcaggaggaa
     3961 ttgtttttga ttctactcca gagggagaat atgccgaaat tcaggccaag ctggcgagta
     4021 tgcgcaaagc actggcccct tttatggtcc aggtatgaaa gtcctcatta ttgataacta
     4081 tgattccttc acatacaatc tgttccaaat cgctgcagaa gctttggagg agtcttcctc
     4141 gcagctggat gttgttcgaa atgatgaact aactgaggaa gagattagac gtaggaacta
     4201 cgaccgaatc attctctcgc caggaccagg cagccccgat gatccggcat atttcggagt
     4261 gtgcgctgcg gttctcacat caatcggcca agttgttccg attctgggcg tctgtctcgg
     4321 gatgcaagga ctcgcgcatt attacggagg caaggtcgta cgcgcacaga tcccgatgca
     4381 cggtaaggtg tctcccatcg agcatgattc ccgcggagtt ttcgccgatc tgccacaggg
     4441 actatcagcg atgagatatc attctctagt cgtggaggag gcgtcattgc cagactgttt
     4501 cgaactcacc gctcgtgctg tggatggagc aagggagtta atggggattc gccacaagac
     4561 gttacctctg gaaggtattc agtttcatcc ggagtcattt gctactgaag gcgggattcg
     4621 gatgatcgaa aattttctct ttcggccgga acgatccggg tctacctaac cttcgaaaat
     4681 gtgcgttcac agtctggcca tacttttgga cactcatcag cagataccgc tttgccaaat
     4741 cgcagttcac cctgcaccct gattctttcg ttccgcctgc gtaccttctt gattcgttcc
     4801 attcgctcca gtgcgctgac cccgagcgaa tggtttgagc gtctgatctt caggtttcaa
     4861 caaagacttc cctcattgat tgcacgggta aggcaaagac cgcgcactag gtatcagcca
     4921 tcctcctcgg atttgacacg atggaatttt cgcatacaca cgcgggtctg gcttcagctc
     4981 aaagtccttg cccgtggcat gaacatatcc atatgttttc tagtgagtat acttttgtcg
     5041 ctcgttcttg aacttggttc cacgactggc aattggaagg tgaagccgtc cgttgcaata
     5101 cgattggaag agcggctcgt tatgtctgtt ggccggattg ttcggaggtt gtgggtggaa
     5161 aagagactta acgttgatac aagttaaaga attcttcaac ctcgagaggc tgatcttgcc
     5221 gctcattctg tgatctgagt agttgctgcc caagggtcgc caggaagggc aagccaaggt
     5281 ctttgtagtg atgatcttca tcattcgcag agcgggcgta gttcacatag agcgttaccg
     5341 tcacgagcaa cgttcgcttg ctttctcgat caatcaagag agccgagtcc gtcaggaatc
     5401 cgagagcctt tccgactttg ttgaaaatcg aaaacttttt cggcaatcgt tctctacttc
     5461 cgcccatgac tagatactta ctcgtgcagc cctgatgatc ttgctcgtca aaatcaagat
     5521 ccgtatcctc cggcattgtt gacatagctt taaggagaag cgttctgctc tcatcgttga
     5581 gggcaaacgt ggccgatgat ggtaggagct ccggcaaaac cgccgccgtt gtgaggcgat
     5641 gaagctcggg aatggagagg aagcttctct ccgtgccatc gtagccgtga acaatatcca
     5701 taccctcgag atgatttgtg tagtgttcgg tggtaacgcg aggttcctgc cgcaagaggg
     5761 tttcgccttc cggagattcg aagtctactc cgttgtagtg ccggttttct tccatactgc
     5821 atttggcggc gagccgatgt gttacctgcc ccgttttgta cccgcgcatg cgcagtccgg
     5881 ccacgattga gtcttgtccc agaaaggaat aaaggcggtc gaatgcttca ttttcgctca
     5941 ccgcgaacat cgcaagaatg tcgcggcgga tcgttgatgg tccaagatcc tcctgctggc
     6001 aatctccagg ctcaagtcgg tacgatgtgt ccagattgat gcccggaatt cctatctttt
     6061 gcactttctc gagtgcaagg agtgcgaccg ggagcttaac cagactggcg gggtaaaagt
     6121 attcctgttc tcgcaaacgg taggaatgca ggatcggttt tagtgttcgt ccatcacgct
     6181 ggaactcggt caccaggatt tgcagtcgat gttgatccgg agtctgcagg attctttgaa
     6241 aaggtaggaa ctccggccgg ctcagcagat tgcgcaaata ggttgaatca tttcgcggca
     6301 tggaatcggt tcgatagatt ggatgtgcca tgcaagcaga atacc
//
LOCUS       DAOQJL010000178         6310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6955_length_6310_cov_3.631655, whole genome shotgun sequence.
ACCESSION   DAOQJL010000178 DAOQJL010000000
VERSION     DAOQJL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_6310_cov_3.631655"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /gene="ligA"
                     /locus_tag="PLG78_09280"
     CDS             <1..1635
                     /gene="ligA"
                     /locus_tag="PLG78_09280"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNE23348.1"
                     /translation="TLVLYYEKGKLERAVTRGTGQVGDVVTENARTIRSIPDRLSEEI
                     DLTVRGEAYMNFDEFDAFNEQQGNIYANPRNLVSGSLKHKKSRETALRPIRWVAFEAY
                     FAKDEKLKTDSSQLDRLSQLGLPVFESHTVSSSGLAAKIELLSKKVKKLNFPVDGLVL
                     KVDDLNLRRDLGYTSASPRWATALKFEPELAQTTVEEIEVFTGRTGRVTPRARLVPVK
                     LAGTTVTYATLHNADYIEKLGVRVGSTVKISKRGEIIPAVEEVVDQGKGPPFSFPKKC
                     PSCGSKLVRGEEAADWLCPNEECDEKLMNAIVFFCQRKQMDIAGLGERNVEILFRKGF
                     VKTIPDLYRLGERKEELEAMEGFGSKSVQIILEGIEASKKQEYRRVLYSLGLREIGPS
                     VTDILLDHGLHSIDDLINAARAKGAVEALQEIHGIGPSTAEAIVEQLNDKRVLAMIDS
                     LRQAGLQFSQKIEKSNLQQIFEGQTWCVTGSFENFKPRDLAMAEVKKRGGKVVSGVSK
                     KTSHLLAGEGAGSKLEQAEKFGTTIVSEEEFLRLIG"
     gene            complement(1642..1905)
                     /locus_tag="PLG78_09285"
     CDS             complement(1642..1905)
                     /locus_tag="PLG78_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HNE23349.1"
                     /translation="MAIELSKEDTQSILPSIIQYMREEFEVELSEMRAGFLLKYFMEE
                     IAPFAYNKGVRDAEQYMQEKVGDLTGVVYEAPLNYWKKKKKGK"
     gene            complement(1924..2385)
                     /locus_tag="PLG78_09290"
     CDS             complement(1924..2385)
                     /locus_tag="PLG78_09290"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNE23350.1"
                     /translation="MATRRTELRFTNYKDIIQDIQRLQKSGYEMTGKWNLGQICRHLS
                     FYWKGSLDGFTVQLPWIIRATLGRMILKSSLKETIRKPGGQTAPQSVFAAEPDDREAV
                     AEAIALLQRLDQNTAKLHPSALFGEVTNDQWKTLHCNHAGHHLSFLVPKGA"
     gene            complement(2387..2935)
                     /locus_tag="PLG78_09295"
     CDS             complement(2387..2935)
                     /locus_tag="PLG78_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE23351.1"
                     /translation="MDPKTKQEIASAMRTQASQLKEFYSSIPDNVFFVRPAKGWSAAG
                     NVRHLKKAAQGVLVGMYTPKGIIRVIFGKSKKPPRTAVQLKELYLRKLGQGAGAGLFS
                     PVNKGGSGNALKKAKLIESFSRATESLAKSLDRFSEEELDQLRLPHPILGMLAIREMI
                     NFGLFHNEHHTGIVRERMKKGN"
     gene            complement(2937..5534)
                     /gene="leuS"
                     /locus_tag="PLG78_09300"
     CDS             complement(2937..5534)
                     /gene="leuS"
                     /locus_tag="PLG78_09300"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNE23352.1"
                     /translation="MSYPFQEIESRWQNHWEKHSIFKTPEDRTRPKFYILDMFPYPSG
                     AGLHVGHPEGYTATDIVARFKRMRGFNVLHPMGWDAFGLPAERYAMKTKIHPSITTEQ
                     NITNFRRQLKALGFSYDWDREINTTDPNYYKWTQWIFLQIFNSWYDTDAGKARPIKEL
                     PIPAELKKKGKEAEKEAFINSKRLAYIAEMPVNWCPGLGTVLANEEVDEWVSKGYTVE
                     RRPMRQWMLRITAYAERLLGDLSTVEWPTGTLELQKNWIGKSTGASIKFAVKDSNHFI
                     EVFTTRPDTLFGISYMVLSPEHPLVEEVTIPAQKSAVESYREEARKKSDLERQLKGEK
                     EEKTGVFTGGYAIHPYTKESIPIWIADYVLMGYGTGAIMAVPAHDERDFAFARKFNLP
                     VKIVVEPVSGKATAEEAFTGEGKSVNSDFISGLPSKEATLKMIERIQADGLGESRVNY
                     KLRDWLFSRQRYWGEPVPVSFTKDGRYVGEKEKNLPLRLPDCEDFQPAETGESPLAKL
                     KDWVEHEDEKLGKLRRETNTMPQWAGSSWYYLRFIEPRNKKALVSEQEEKYWMGETGV
                     DLYVGGAEHAVLHLLYARFWHKVLFDLGHVHSIEPFHKLVHQGLILGEDGQKMSKSLG
                     NVVNPDDVLVEFGADSFRLYEMFLGPLEMSKPWSTKAIGGVYRFLSRVWRLYTARGAG
                     DSDEDGENARSSAHSPLDEVLLVEPEGDRRVKVERLLHQTIRKVTEDIEKMSFNTAVS
                     ALMVFVNETTAEKKIGRDAAEKFLLLLSPFAPHIAEELWSKLGHNESIAHSAWPGFDL
                     TKTQEDEIEIVFQVNGKVRGKAKMATGSSDDQMKTAALADEAVLRAMEGKEPRKVIVV
                     KNKLVNLVV"
     gene            5546..>6310
                     /locus_tag="PLG78_09305"
     CDS             5546..>6310
                     /locus_tag="PLG78_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23353.1"
                     /translation="MEKGPWIEFFPVGRFGQADNQNSMVRGLVCVFMLVFSGALHAEG
                     GELIYAFRNYTRPDPIKMLLVGEIQSKIKAPRITEQQSPFSGYDTRLDQVTIKVRNPT
                     GLKVGQKLYIIDKNPHHEKFRNGLIVGEITVQSVLKTSFYGWVVTGTGILLRVREGLF
                     VARTQESENLVHAFDLKKKGDFYADSGAFDKAVATYQASLVADPGLPEAHAALGTLFY
                     QESKKGSHEFPSRSLSEFEQAWKVRENFRYRYDEMAF"
BASE COUNT         1500 a         1553 c         1552 g         1705 t
ORIGIN      
        1 acacttgttc tttactatga gaagggaaag cttgaacgcg cggttacacg cggaacgggg
       61 caggtgggag atgtggtcac tgagaatgca cgcacgattc gcagcatccc ggaccgcctg
      121 agcgaagaga ttgatctgac tgtacgcgga gaagcgtaca tgaacttcga tgaatttgat
      181 gcattcaacg agcagcaggg aaatatctac gccaatccac gcaatctcgt atctggttct
      241 ttgaagcata agaagtcccg cgaaactgct cttcgaccca ttcgctgggt tgcctttgaa
      301 gcatacttcg cgaaagatga aaaactaaaa acggattcat cgcaactgga tcgtctttcc
      361 caactggggc ttccagtgtt cgaatcccac acagtgtctt cttccggtct cgcagcaaaa
      421 atagaattgt taagtaaaaa agttaaaaag cttaactttc ccgtggatgg tcttgttctg
      481 aaagtagacg acctgaatct gcgtcgtgat ctgggatata cttctgcttc ccccagatgg
      541 gccacagctc ttaagtttga acccgagctt gcacagacaa ccgtagaaga gattgaagta
      601 ttcacgggta gaaccgggag ggtgactccc agggccaggc tggttcctgt aaagctggca
      661 ggcacaactg ttacgtatgc tacgcttcac aatgcggact acattgaaaa actcggagtg
      721 cgtgttggtt ccacggtcaa gatttccaag cgcggcgaaa tcattcctgc agtcgaagaa
      781 gttgtagatc agggcaaagg cccgcctttc tcttttccta aaaaatgtcc ctcctgcggc
      841 tcaaagctcg tacgcggtga ggaagcggca gactggcttt gtccaaacga agaatgcgac
      901 gaaaagctaa tgaacgcaat cgtctttttc tgtcagagaa agcaaatgga tattgcggga
      961 cttggagagc gtaacgttga gattctattt cgcaaaggat ttgtgaagac aatccccgac
     1021 ttataccggt taggcgaacg caaagaagaa ctcgaggcca tggaagggtt tggatccaaa
     1081 tccgtgcaga tcattctgga aggaatcgaa gcatctaaga agcaggaata ccgcagggtg
     1141 ctttattcac tggggttgcg cgaaataggt ccaagtgtca cagacattct cctggaccat
     1201 gggcttcata gcattgacga tcttataaac gcagcgcgcg caaaaggtgc cgtagaagct
     1261 ttgcaggaaa ttcatgggat cggcccaagc actgcggagg cgattgtaga gcagctgaac
     1321 gacaaacgag ttcttgccat gatcgattct ctaagacagg cagggcttca attctcgcag
     1381 aaaatcgaga aatcaaatct acagcaaatt tttgaagggc agacgtggtg cgtgactggc
     1441 agctttgaga atttcaagcc acgcgatctt gccatggcag aagtgaagaa gagaggcggg
     1501 aaagtagttt ccggtgtttc aaagaaaact tcgcatctac tggccggaga aggcgcgggg
     1561 agcaagttag agcaagcaga gaagttcggc actacgattg tcagcgaaga agagttcctg
     1621 cgtttgatcg gctgatggcc gctatttccc tttcttcttc tttttccagt agttgagcgg
     1681 ggcctcatac acgacgccgg tcagatctcc taccttttct tgcatgtact gctccgcatc
     1741 gcgtacaccc ttgttgtacg cgaatggagc gatttcttcc atgaaatatt tgagtaagaa
     1801 tccggcccgc atttcgctga gttccacttc aaactcttcg cgcatatatt gaatgatgga
     1861 aggaagaatg gactgcgtgt cctctttgct cagttcgata gccatagggt tctccttgta
     1921 acgttaagcg ccctttggca ccagaaaact caaatgatgt ccggcatgat tgcagtgcaa
     1981 ggttttccac tgatcgttgg tcacttcccc gaaaagagca gatgggtgga gcttcgccgt
     2041 gttttgatcc aacctttgca gaagtgcaat tgcttctgcc accgcttctc tgtcatccgg
     2101 ctctgcggcg aatacagatt gaggcgcggt ttgtcctcct ggcttgcgaa tggtttcctt
     2161 cagcgagctt ttgaggatca ttcttcccag agtggcgcgg atgatccagg gaagttgcac
     2221 tgtgaatcca tccagggagc ccttccagta gaaactgaga tgtctgcaaa tctgtcccag
     2281 attccatttg cctgtcattt catatcctga cttctgcaag cgctggatgt cctggatgat
     2341 gtctttgtaa ttggtaaagc gaagctccgt tcttctggtg gccatgttag tttcctttct
     2401 tcattctttc gcgcacgatg ccagtgtgat gctcgttgtg aaacaaacca aagttgatca
     2461 tttcgcggat agcaagcatc ccgaggatgg gatgcggtag gcgaagttga tccagttctt
     2521 cttctgaaaa acgatcgagt gatttcgcaa gtgattctgt tgccctgcta aacgattcga
     2581 tgagctttgc tttcttcagc gcatttccag acccaccctt atttacagga gaaaacaatc
     2641 ctgctccagc accctgtccg agtttgcgaa gatagagctc cttgagctgg actgctgtcc
     2701 ggggtggctt cttgcttttc ccaaagatga cgcgaataat accctttgga gtgtacattc
     2761 caacgagaac tccctgcgct gcttttttca aatgacgtac gttgcctgct gcagaccatc
     2821 ccttcgccgg tcgtacaaaa aagacgttat ccggaatcga tgagtagaat tctttcaatt
     2881 gcgacgcctg ggttctcatc gcggaggcga tttcttgttt tgtcttagga tccactttac
     2941 accaccagat tcacgagttt gttcttcact acgatgactt tgcgaggttc ttttccttcc
     3001 atggctcgca aaaccgcttc gtctgcaagt gcggcagttt tcatttgatc gtctgatgat
     3061 ccggtcgcca tctttgcttt gccgcgcact ttcccattca cttgaaacac gatttcgatt
     3121 tcgtcttcct gcgttttcgt cagatcaaat ccaggccagg cggagtgtgc aatggactcg
     3181 ttgtgaccaa gcttactcca gagttcttct gcgatgtgag gcgcaaaagg agaaaggaga
     3241 agtagaaatt tttcggcagc atcgcgtccg atttttttct cagcagtggt ttcatttacg
     3301 aacaccatga gagcagacac tgcagtattg aaagacatct tctctatgtc ttctgtcact
     3361 ttgcgaatgg tctggtgaag aagcctctct acttttacgc gcctgtctcc ttctggttcc
     3421 acaagcagaa cttcatccag aggcgaatgc gcactcgatc ttgcattttc tccgtcttcg
     3481 tctgaatctc ctgcaccgcg cgcggtgtag agcctccata cgcgcgatag aaaacgatac
     3541 actccaccga ttgctttggt agaccacggt ttgctcatct ccagtggacc caggaacatt
     3601 tcgtagagac ggaatgaatc tgctccaaac tcaacgagaa cgtcgtccgg atttacaaca
     3661 ttgcccagag acttggacat tttttgtccg tcttcgccca ggatgagtcc ttgatgaacg
     3721 agtttgtgaa atggttctat ggaatgaaca tgccccagat caaagagcac cttgtgccag
     3781 aacctcgcgt agagcaaatg aagaaccgcg tgttcagcgc cacccacata cagatcgact
     3841 cctgtttctc ccatccagta tttctcttcc tgttcggaaa caagagcttt cttattgcga
     3901 ggctcaatga acctcaggta ataccaggag cttcccgccc attgaggcat tgtgtttgtt
     3961 tcgcgacgaa gtttgcccaa tttttcatct tcgtgctcta cccagtcttt tagcttcgcc
     4021 agaggcgatt ctcccgtctc tgcgggctgg aaatcttcgc aatcaggaag gcgaagcgga
     4081 aggttctttt ccttttctcc tacatatctt ccatcttttg taaacgacac aggcacaggt
     4141 tctccccagt atctctgtct tgaaaaaagc cagtcgcgca atttgtaatt cacacgactt
     4201 tctccgaggc catctgcctg gattcgttcg atcattttca gtgttgcttc tttggaagga
     4261 agtccggata tgaaatccga gttcacactc tttccctctc ctgtgaatgc ttcttccgcg
     4321 gttgcctttc cgctcacggg ttccactacg atcttcacag gtagattgaa ctttcgcgcg
     4381 aatgcgaagt ctctttcatc gtgagctgga actgccatga tagcgcccgt gccgtatccc
     4441 atgagcacat agtctgcgat ccagatgggg atggactctt ttgtgtaagg gtgaatcgca
     4501 taaccacccg taaacacacc tgtcttctct tctttttctc ctttgagttg tcgctcaagg
     4561 tctgactttt ttctggcttc ttctctgtac gattctacag cgctcttctg ggccgggatg
     4621 gtcacctctt ctaccagagg atgttccggg gatagaacca tgtaagagat gccgaataac
     4681 gtgtcgggtc tggttgtgaa tacttcaatg aagtggttgc tatctttgac cgcgaatttg
     4741 atagaagccc ctgtggattt cccgatccag tttttttgca gttccagagt tccggtaggc
     4801 cattccacag tactcagatc gcctaacaga cgttctgcgt atgcggtgat tcgcagcatc
     4861 cactgacgca tgggtctgcg ttctacggta tagcctttgc tgacccattc atctacttct
     4921 tcgtttgcaa gcacggtgcc aaggccggga caccagttca caggcatctc tgcgatgtag
     4981 gccagtcgtt tcgaattgat gaatgcctct ttctccgcct cttttccttt ctttttcagt
     5041 tctgcaggaa tgggaagttc tttgatggga cgtgctttgc ccgcatctgt gtcataccag
     5101 gaattgaaaa tctgcagaaa gatccattgt gtccatttgt aatagtttgg atccgttgtg
     5161 ttgatttcgc gatcccaatc gtatgaaaaa cccagagcct tcagttgcct gcgaaagttc
     5221 gtgatgtttt gctccgtagt aatggatgga tgaatctttg ttttcatcgc ataacgttcc
     5281 gctggcaaac caaatgcgtc ccagcccatt ggatgcagaa cgttgaatcc tctcattcgc
     5341 ttaaagcgcg ccacaatgtc cgtcgcagtg tatccttccg gatgaccaac gtgaagccct
     5401 gcacccgaag ggtaggggaa catgtccagg atgtagaatt taggacgagt tctgtcctct
     5461 ggagtcttga aaatggaatg cttttcccag tgattttgcc agcgggattc gatttcctgg
     5521 aaagggtacg acatacggcc agaaaatgga aaaaggcccc tggatcgagt tttttccggt
     5581 gggccgtttc gggcaggccg acaatcaaaa cagtatggtg cgcggtctcg tttgcgtctt
     5641 tatgctcgtc ttttcgggtg ctttgcacgc ggaaggagga gagctgatct acgcatttcg
     5701 caactacact cgcccggatc caatcaagat gctcctcgtg ggagaaatcc agagtaagat
     5761 caaagcgcct cgcattacgg aacagcaatc tcccttctcc ggctacgaca ctcgcctgga
     5821 ccaggtcacg atcaaggtgc gaaacccgac cggactcaag gttgggcaaa aattgtatat
     5881 aattgataag aatccgcacc acgagaaatt caggaacggc ctgattgtgg gcgaaatcac
     5941 agtacagtct gtactgaaaa caagcttcta cggctgggtt gtaacgggca ccggaattct
     6001 acttcgcgtc agagaaggtc tatttgtagc gcgcactcag gaatccgaaa acctggtgca
     6061 cgcctttgat ttgaaaaaga aaggcgattt ctacgcggac agcggcgcgt tcgacaaagc
     6121 agtggctacg tatcaggcgt cgcttgtggc agatccggga cttccggaag cacacgcagc
     6181 cctgggaact cttttttatc aagaatcaaa gaaaggaagt catgaatttc cttcgcgatc
     6241 tctgtccgag ttcgagcagg catggaaggt gcgcgaaaac tttcgatata gatacgacga
     6301 aatggctttc
//
LOCUS       DAOQJL010000179         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_6960_length_6305_cov_4.393440, whole genome shotgun sequence.
ACCESSION   DAOQJL010000179 DAOQJL010000000
VERSION     DAOQJL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_6305_cov_4.393440"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1528
                     /gene="sucC"
                     /locus_tag="PLG78_09310"
     CDS             311..1528
                     /gene="sucC"
                     /locus_tag="PLG78_09310"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNE23354.1"
                     /translation="MKIHEYQAKDILRRYGVTVPEGRVTGNPAEIGKLYDELGGGIVA
                     VKSQIHAGGRGKGIVYDKEDPATATKIQDGGVKVAKSREEAEKYGSNIVGNYLVTQQT
                     GGVGKRVLKVYLEKGTEIAREFYLSILVDRAVSKPIVIYSTEGGMDIEEVAAHTPDKI
                     HRLEVEPAIGLQAWQAREILYKLGVPAEAMKQGVDFLLKLWKACDDEDASMLEVNPLV
                     LTKQNTIIALDCKFTYDDNALYRHPEAVHLRDITEEDPLELRASEFNLNYVRLDGNVG
                     CMVNGAGLAMATMDIIKLAGEFPANFLDVGGGANAQTVANGFRIILGDPNVKAIFINV
                     FGGIVQCDRVANGIIQAAKDVNIHVPLIVRLKGTNADIAKDILAKSGLKILVASELKD
                     GADAVATAIRGGK"
     gene            1539..2411
                     /gene="sucD"
                     /locus_tag="PLG78_09315"
     CDS             1539..2411
                     /gene="sucD"
                     /locus_tag="PLG78_09315"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE23355.1"
                     /translation="MAVLVDKNTRVVVQGITGKEGSFHTSQMIEYGTRVIGGVTPGKG
                     GQKSEHGVPIFNTLQDAVDKEGANATVIFVPPPFAADAILEAVGAQIPLCICITEGIP
                     VRDMSRVYSVLKTSKTRLIGPNCPGVISPGKCKIGIMPAMIHKEGRIGVVSRSGTLTY
                     EAVGQLTIQGFGQSTCIGIGGDPIIGTKHKAAVELLMNDPETDGIIMIGEIGGTDEED
                     AAEWIKLNKKKPVVGFIAGQTAPPGKRMGHAGAIISGGKGTASTKMEAMRNAGIVVVE
                     SPAEMGAAMKKALG"
     gene            complement(2717..3928)
                     /locus_tag="PLG78_09320"
     CDS             complement(2717..3928)
                     /locus_tag="PLG78_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNE23356.1"
                     /translation="MKQILVLGGNFAGVTAALEIQRKLGKEAEVTVISPSEKFLYVPS
                     LIWVPFGARKVQDISFQIGPVLSRKGVKFVLDRATKIRPDANIVETAASGEFKYDYLV
                     AATGASLGFDAVEHLDPKDGFVNCIVTPPLAEKAYDAFREIVKNPGPVIVGATQGASC
                     MGAAYEYLFNLDKYLRKAGVRDKVEITWVTPEPFLGHFGIGGVMGGQKMLELFMKMYG
                     IKWHTNATVSKIEKEKLTLGDGTAIPYKLAMLIPPFRGADVMRNSPELVDDKGFVVTN
                     EGYQHIKYKNVYAAGLAVEVIAPFTKCAAPFGVPKTGFPSDVMGKIVAENIKHDMHGT
                     GKFKKLAFGKIPGICIMDAGKKEVWILTNHLFKPRQFEIMIPNMVYNFGKVLLEKYMI
                     WKNRSGLAWMP"
     gene            complement(4016..5095)
                     /locus_tag="PLG78_09325"
     CDS             complement(4016..5095)
                     /locus_tag="PLG78_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE23357.1"
                     /translation="MEDFILALLSSSAILLSAVIVLALSLRRRTAHLTTLRAEVEKAF
                     GRVPSEPDEMAGIVHRLESDLADFTRTRDSIGRIISFIDGLLQESENQTQALELISPN
                     LQVMSFSMQNIADSAQKQLETIENTGKVFSDFNESTGELFSVISVINDKSIETLSHAK
                     RGTETILGSFQSMDEIHQSLSRIGEIIKTIRNIADKTNLLSLNAAIEAARAGEQGRGF
                     AVVADEVSKLADKSTQSVKQIAGMLRENLETSVQGKEVSDQARSSFDEISGSILDMNE
                     QVQEVNAYVSSEKRQMLDLESQIHTLKNASLEISASIQNQSIQAKELVRSIEILFETT
                     KSVSAGVRSVSQLLEEIRHDPTLSP"
     gene            5204..6013
                     /locus_tag="PLG78_09330"
     CDS             5204..6013
                     /locus_tag="PLG78_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE23358.1"
                     /translation="MHVHLFPAPVFRAIWRFFESSSNGLWNIRYRLHGDDHVRHLEEQ
                     GIRKFTGLLYAHKPGMADSLNDFMAESAQRYSGLLPFGTIYAADGDVAARSRRIFEDY
                     GFYGIKLHPFVSREQLDDRGFYPAYEIMEDLGRVLVCHPASAPVYDATDGARRISNIL
                     SDFPGLRIVIAHCGAFETREYLELADRHEHVYFDTAMNCVHTHVFENNCPGREFFLRY
                     QDRIVYGSDYPNIPYEYQEQRMSIQALGLGPEIEKKIFENNATRLLRLEKV"
     gene            6010..>6305
                     /locus_tag="PLG78_09335"
     CDS             6010..>6305
                     /locus_tag="PLG78_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE23359.1"
                     /translation="MNSDIVKKLEERRQAALAMGGEASVAKHRAKNRLNVRERLNVLF
                     DAGSFLEYGLHADHAEVSTELHGKHAAADGVVTGVGRVDGRDVFCIAYDFTVLAG"
BASE COUNT         1559 a         1546 c         1666 g         1534 t
ORIGIN      
        1 agtgaatcat catcgattcg tggttcccca tcaggaaggt ggatcgctct ttttcgcgta
       61 agcatctttc gattaccccg gcagagtcgg gtccgcgatc cacataatcg cctaaaaaca
      121 gcagtggttc gtgctccgga atgcggtcga gcagagcatc cagttctctc aagcaaccgt
      181 gtatgtctcc gatgatccac atagcgaaat aagcacgatg gcggattgaa aaagaatttt
      241 caacacaccg tgtccgcaca aattagcacc tgcttctgca gaggaaacaa aaacagcaca
      301 ggactgaacc atgaaaattc atgaatacca ggcaaaagac atactgcgac gctatggcgt
      361 gacagttcca gaaggaagag tcacgggcaa tcccgcggaa atcggcaagt tgtatgatga
      421 gcttggcggc ggcattgtag ccgtaaaatc acagatacac gcaggcggac gcggaaaagg
      481 catagtatac gacaaagaag atcccgcaac agcgaccaaa attcaggacg gcggcgtaaa
      541 ggtcgcaaag tctcgcgaag aagctgaaaa atacggctcc aatattgtgg gcaattatct
      601 agtaacgcaa cagaccggcg gagtcgggaa acgagtgctt aaagtatacc tcgagaaggg
      661 cactgagatc gcgcgcgaat tctacctttc cattcttgta gatcgcgcag tctccaagcc
      721 aatcgtgatc tattctacag aaggtggaat ggacattgag gaagtggcgg ctcacactcc
      781 agacaagatc catcgtcttg aggtggaacc ggcgatcggg ctgcaggcct ggcaggcgcg
      841 cgaaatctta tacaagctcg gagtccccgc tgaagcaatg aagcagggcg tggacttcct
      901 cttgaaattg tggaaagcgt gcgacgacga agatgcatcc atgctggaag tgaatcctct
      961 ggttctcaca aagcaaaaca caatcatcgc tctggattgc aaattcacgt atgacgacaa
     1021 tgcgctttac cgtcatcctg aagcagttca tctccgcgac atcacggaag aggatccact
     1081 ggaacttcgc gcatctgaat tcaatttgaa ctacgttcgc ctcgatggta acgtaggatg
     1141 tatggtgaac ggtgccggtc ttgccatggc aaccatggat atcatcaaac tggcaggtga
     1201 attcccggca aacttcctgg acgtcggtgg tggagcaaat gcacagactg tggcaaacgg
     1261 atttcgcatc attctgggcg atccaaatgt aaaagcaatc ttcatcaacg tttttggtgg
     1321 gatcgttcaa tgcgaccgtg ttgccaatgg catcattcag gcagcgaagg atgtaaacat
     1381 tcatgttccc ctcatcgtga gattgaaagg aaccaacgct gatattgcga aagacattct
     1441 tgcgaaatcg ggtctgaaga tcctggttgc aagtgaactc aaagacggcg cggacgcagt
     1501 ggctactgct attcgcggag gaaaataagg aacggtttat ggcagtacta gttgataaga
     1561 acaccagagt agtggtccag gggatcacag gtaaagaagg atcgtttcat acgtcccaga
     1621 tgattgaata tggaactcgc gtgatcggcg gtgtcactcc cggaaaaggc ggacagaaga
     1681 gcgagcacgg agtgcccatt ttcaatacgc ttcaggatgc agtggacaaa gagggcgcga
     1741 acgctacagt gatttttgtt ccacctccat tcgcagcgga tgcgattcta gaagcagtcg
     1801 gtgctcagat tcctctttgc atttgcatta cagagggaat cccggtgcgc gatatgtccc
     1861 gcgtatattc tgttctcaag acttcaaaga ctcgcctgat tggaccaaac tgtccaggtg
     1921 tcatcagccc tggcaaatgc aagatcggga ttatgcctgc gatgattcac aaagaaggta
     1981 gaatcggtgt ggtgtctcgt tctggaactc tcacctatga agcagtaggc cagcttacga
     2041 ttcagggatt tggtcagagc acgtgcattg gaattggcgg agacccgatc atcggcacga
     2101 agcacaaggc ggcagtagag ttgctcatga atgatccgga aacagatgga atcatcatga
     2161 tcggcgaaat cggaggcacg gacgaagaag acgcagcaga atggatcaag ctcaataaga
     2221 agaagcctgt cgttggcttc atcgcaggcc agactgcacc tcccggaaaa agaatgggac
     2281 atgcaggcgc catcatcagc ggtgggaaag gtacagcgtc cacaaaaatg gaagcgatga
     2341 gaaacgctgg cattgttgta gtggaatctc ccgcagaaat gggcgcggcc atgaagaagg
     2401 ctctgggcta aacgaaagtc cattccttgc aaccacggaa agcctccgct cctaaagcgg
     2461 gggctttttt tttgccctcc ctccgaagcg accgaagaat gcaacagcca gcagcagccg
     2521 acagtttgta gcgcgggcgc ggctggtgca tgggacttcc cagaatatgg tactttgcaa
     2581 gtgagacagg ggtcggttga cttctcgcgc cgatctgcaa catggcgccg caggtcggcc
     2641 ggctcgctcc gaggtggggc actctttgaa tgcacccacc accaaaatcc acccacaggg
     2701 ggtgggggag gactgttcag ggcatccagg cgagcccgct gcggttcttc cagatcatgt
     2761 atttctcaag aaggaccttg ccaaagttgt agaccatgtt ggggatcatg atctcaaact
     2821 gacgcggctt gaagaggtga ttggtcagaa tccacacttc tttctttccc gcatccatga
     2881 tgcaaatacc cgggatctta ccaaaagcaa gtttcttgaa cttacctgtt ccatgcatat
     2941 cgtgtttgat attctctgcg acgatcttgc ccatcacgtc ggacgggaag ccggtcttgg
     3001 gcactccaaa cggggcggcg cattttgtga aaggagcaat cacttctaca gcaagacctg
     3061 cggcatacac attcttatat ttgatatgct gatagccttc gtttgtaacg acgaaacctt
     3121 tgtcgtctac aagttcgggt gaattgcgca taacgtccgc cccgcgaaac ggaggaatca
     3181 acatcgcaag cttgtaagga atcgccgtcc catcgccgag tgtaagcttt tctttttcaa
     3241 ttttggaaac cgttgcattt gtatgccact tgatgccgta cattttcatg aagagctcga
     3301 gcatcttctg tccacccatt actccgccga ttccgaaatg tccgaggaag ggctcaggag
     3361 tcacccatgt gatctccact ttgtcgcgca ctccggcctt gcgtaggtac ttatccagat
     3421 tgaacaaata ttcgtaagcg gcgcccatgc aactggctcc ctgcgtagct cctacaatga
     3481 cgggaccagg attcttcacg atctcgcgaa acgcatcgta tgctttctca gccagaggag
     3541 gtgtgacaat gcagtttaca aatccgtctt ttggatccaa atgctccact gcatcaaaac
     3601 ccagagacgc gcccgttgcc gcgaccaggt agtcgtactt gaattccccg gacgccgctg
     3661 tttctacaat gtttgcgtcc gggcgaattt tcgttgcgcg atccagtacg aatttcacgc
     3721 ctttgcgact cagcaccggc ccaatttgaa acgaaatgtc ctgaaccttt cttgctccaa
     3781 acggaaccca gatgagggaa gggacataca gaaacttttc agaagggctg attacagtga
     3841 cctcggcttc tttgcctagc tttctttgga tttcgagggc cgctgttact ccagcaaagt
     3901 ttccacccag tacgaggatt tgtttcatgc tttcaatata gggtatgggg tatcctatgc
     3961 aagcacaaac gtgcgatgcg actgcggatt tcgtatgatt tttgtcatgg aatgtttacg
     4021 gagaaagggt ggggtcgtgt ctgatttctt ccaggagctg gcttacggac cggactcctg
     4081 cagacacgga cttcgttgtt tcaaaaagga tttcaatgga tcgaaccagt tctttggcct
     4141 ggatagattg attttgaatg gaagcagaaa tctcgagact tgcgttcttc aaagtgtgga
     4201 tttgactttc tagatccagc atctggcgct tctcggaact cacgtatgcg ttgacttcct
     4261 ggacctgttc gttcatatcc agaatgcttc cagaaatttc gtcgaagctg gaccttgcct
     4321 gatcggatac ttcctttcct tgcacagaag tttccaggtt ctctctgagc atgcccgcga
     4381 tctgctttac agactgcgtg ctcttgtccg cgagcttgga aacctcgtcc gccaccactg
     4441 caaatccacg accctgttcc cctgcgcgcg ccgcttcgat ggctgcattg agcgagagaa
     4501 ggttggtctt atctgcgatg ttacgaattg tttttatgat ttcgccaatt ctcgaaaggc
     4561 tctggtgaat ttcgtccatg gactggaaac ttcccaggat ggtttctgtg ccgcgtttcg
     4621 catgcgatag agtctcgatc gatttgtcat tgatgactga gatgacgctg aagagttcgc
     4681 ctgtgctttc gttaaagtct gagaatactt tgcctgtgtt ttcaatggtt tcgagttgtt
     4741 tctgagcgga atctgcaatg ttctgcatag agaagctcat cacctgaagg ttcggagaaa
     4801 tcagttcaag tgcctgcgtc tggttctctg actcctggag gagcccgtca atgaacgaaa
     4861 tgattctacc aatggagtcg cgcgtcctgg tgaagtctgc cagatcagac tccaggcgat
     4921 gcacgatgcc tgccatttca tccggttcag atggcactct gccgaacgct ttctctacct
     4981 cagcgcgtag agtggtcagg tgggccgttc gtcgcctcag agagagggcc agaacgatca
     5041 cagcagacag cagtatggca gaactgctca gaagtgccag aatgaaatct tccatggggc
     5101 cctgggtctt attttcggca tttcgagccc tctgaccgcg ctctcgtggg tttttttggt
     5161 ttgccgacca ggcgcctcga gggagggtcg agcgttgatt gacatgcatg tccacctctt
     5221 cccggccccc gttttcaggg cgatctggag gttttttgag tcgagttcca atggtctgtg
     5281 gaatatccgc taccgcctgc atggggatga ccatgtgcgt catctggaag aacagggaat
     5341 ccggaagttc acaggtttgc tttatgctca caagcctggc atggcggatt ctctcaatga
     5401 cttcatggca gaatcggcac agcgttattc tggccttttg ccattcggca ccatctacgc
     5461 tgcagatggc gatgtagctg cccgatccag acgcatcttc gaagactacg ggttttatgg
     5521 aatcaaactg cacccttttg tttcgcgcga gcagctcgat gaccgtggtt tttatccagc
     5581 atacgaaatc atggaggacc tggggcgagt gctggtctgt catcccgctt ctgcacccgt
     5641 atacgatgcc acagatggag ctcgacgcat ctcaaatatt ctgtctgact ttccaggcct
     5701 tcgcattgtg atcgctcatt gcggcgcatt tgaaaccaga gagtatctgg aactggcaga
     5761 tcgtcatgag cacgtatatt tcgacactgc gatgaactgt gtacacacac atgtatttga
     5821 aaacaattgt cccggtcgag agttcttcct gcgatatcag gacagaatcg tttatggctc
     5881 cgattatcca aatattccat acgaatatca agagcagcga atgtcgattc aggcactcgg
     5941 gcttgggccg gagattgaaa aaaagatatt tgagaacaac gcaacgcggc tgcttcgtct
     6001 ggagaaagta tgaattctga tatagtaaag aaacttgagg aaaggagaca ggccgcactc
     6061 gcgatgggtg gagaagcttc tgtagcaaaa caccgtgcga agaaccgttt gaacgtgcgg
     6121 gaaagattga atgttctatt tgatgctgga tctttcctcg aatacggact ccatgccgat
     6181 cacgcggaag tatccactga actgcacgga aagcacgcgg ccgcagatgg cgtggtgaca
     6241 ggggttggta gagtggacgg ccgcgatgtg ttttgcatcg cctatgattt cacggtgctt
     6301 gccgg
//
LOCUS       DAOQJL010000180         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7022_length_6268_cov_4.282150, whole genome shotgun sequence.
ACCESSION   DAOQJL010000180 DAOQJL010000000
VERSION     DAOQJL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_6268_cov_4.282150"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLG78_09340"
     CDS             <1..315
                     /locus_tag="PLG78_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE23360.1"
                     /translation="VLVMKDGRPSGMITAFDLLRLGYEEVFDPDRDYLKTTVGAVLGD
                     RPLPSVSSGTKLWELLNMMIEQNLRTVAVVDDETIKGVVSMVDLAKWYRNTHQEIRTG
                     HL"
     gene            302..649
                     /locus_tag="PLG78_09345"
     CDS             302..649
                     /locus_tag="PLG78_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE23361.1"
                     /translation="MATSDFLPHYGPRLDERELAREKDKARKLRDSPWWRKKKQSGVC
                     YYCRKKFKPADLTMDHLIPLARGGRSVKENLVPCCKDCNNKKRYYIPAEWNDYLKSLR
                     GEEPPPENQEIST"
     gene            646..2805
                     /locus_tag="PLG78_09350"
     CDS             646..2805
                     /locus_tag="PLG78_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNE23362.1"
                     /translation="MRQAFFCAWIAFGLLGACKSTYPGGIPASADPASALTACSTSGL
                     ETTGVKRVAVFVFDPGGKEEALRMKVLDAYAAGFLRDKRFRPVEREKLGTILREQGLS
                     SAGLLTGEDAAKMGGVLPVDWIVSATVMRSSGKVTVSGRFLDAVNGSILQGFDCPFLV
                     DSIAADQSVQISQNITNSNNTTNSNNTTTSSTITSNTINSNNTTNSNNQSTNINIGVL
                     NVTDPTRIIQKKCDSVHAPVIAALRNLTSPELVERAARLAMKIRFDMECGQIHFLVMD
                     TFERYGFAPPEYGDFLIRTMDGIDDVSDDSRTQSILRYLAATDGRVDQAEWKSGFSAL
                     MRARTGSVTGYLIYLLNHRREGMIAVDRAREVLSAADAGRLGRPVPAKGGQILYSVLS
                     ALKFDSPAGSGPFVSVLSQNSEKYAAYQYERLSFASLVSLVTAYYRYADEDGRTVMRQ
                     SLARFFAGQKNQSDRAHEHLFYDIAYGLEREANGEDGESPDKRNRAARELQSALIDLH
                     DPMCRAFTLYDRPTLSTRVDQIHFLLRNGAACPGLPTVSELISKVEAGDWDEKASAFR
                     TLVEMRGNAKQAEAVAIRYFKIEGMSRSSEIRAYCAQILGYTRTTNPEAHQLLIAGAG
                     DFDSPVQDSSEKALGRLGLLVKPAILRYLMVPRTRQDQVREIRRRMAMVQTLGRMGRD
                     ASDAIPVLNQIAATDVDSYLQRMAKTAIVNIQENRTE"
     gene            complement(2818..4296)
                     /locus_tag="PLG78_09355"
     CDS             complement(2818..4296)
                     /locus_tag="PLG78_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE23363.1"
                     /translation="MILASPGHSEVLLEIEGSTGPVRILFDAWLSDFAFGDFMQRNPR
                     VDLGSIGKLDAVYISHSHCDHLDPYTLTALYQKQTPVLVVPETIEFLVPLLSKFLPGA
                     SIEVLRHNKTSNICGLSWTGFAFAVPYLTNEEDVMPLVIRSKKESVFFEADIALPDSQ
                     EAHGAVDRAMGETERVFVSTRNELEALYASYDAKTPQERKQKLTAYRRRRKDELYWEY
                     ARYAELELPCPFRPGSLKLITGQGMIFPVDLSREFLPLSNPFPLADVTALEVQAARET
                     GYDLKLMTLEAGEQIDSRTIKKSKSQIKVEQTRLTQDPAAPAPAPRPQGPLFDDLRDH
                     SLQKQRILHILNNRFVPHMAYHREEPLKALLLARGSYTIRILYGTSSSHRHEDYTIRF
                     ADMTFRELKEQNKQEGSAGRTKAKPDEIYWANDLEDFLNGRQDQFSTTLHNFFEGTAI
                     RLWTMLGLPFLNNDLIEAKVGFHFERAASGHTVNDWIEQIKK"
     gene            complement(4293..5534)
                     /locus_tag="PLG78_09360"
     CDS             complement(4293..5534)
                     /locus_tag="PLG78_09360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE23364.1"
                     /translation="MAPDYITMAIPVFLFLIAVEIIVSRLKKGGYYDYHDSVNDLSAG
                     ILSQVVGVFGKIITVGLYVYVYQNWRLLEISEKSVIAWVACFFGVDFFYYWFHRIAHE
                     SAVFWGSHLPHHQSEEYNLTVALRQGAFQSFFSIWFYVPLALVGIPPLIFLVHSQFNT
                     IYQFWIHTRAVGKLGFLEIFMNTPSHHRVHHGKNPIYIDKNYAGTLIIWDKMFGTFQE
                     ETEEVHYGTIKPLASWNPIWAQVHYFLELFQKSIKAPRFLDKILLWFKGPGWLPTGLE
                     YDAFMADPAYSPEGMKNRVPARYPAKYRTKLSTALEIYTFVNFLFTLGFASYFFFMSP
                     TMLLGAKLFYGGFVALSLLTLGGIFESKRWAHILEIHRLFIGALMIALYGGDIGFLAA
                     IPIMMAVFFYLQVFRTEMKKA"
     gene            5672..>6268
                     /locus_tag="PLG78_09365"
     CDS             5672..>6268
                     /locus_tag="PLG78_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE23365.1"
                     /translation="MKTVVIDGVTVQLASPDLIDQNWVGNMDVLNQLMACWVLVHPSD
                     LPLNPRLIGRPGVGKTTLACAAARVLDKPTYIFQATMDTRPEDLLITPVISESGKIKY
                     VASAIVTAMLEGGIAILDEGNRMGEKSWASLAPLLDHRRYIESIVAGIKIKASPEFRF
                     CATMNEDASTYEIPEYIQSRLQPQIYIDFADADEELEIL"
BASE COUNT         1617 a         1469 c         1641 g         1541 t
ORIGIN      
        1 gtactggtca tgaaagacgg aagaccctct ggcatgatca ctgcatttga tttactcagg
       61 ctcggctatg aagaagtgtt cgatccagat cgcgattatc taaagacaac ggtgggtgcg
      121 gtcctgggag atcggccact tccgtctgtg tcttccggaa ccaaactatg ggaattgctc
      181 aacatgatga tagaacagaa cctacgcaca gttgctgttg tggacgacga gacaatcaaa
      241 ggtgttgttt ccatggtgga tctggcgaag tggtatagaa atacgcacca ggagatccgt
      301 actggccacc tctgattttc ttcctcatta tgggccacgt ctcgacgaaa gggaactggc
      361 ccgagagaaa gacaaagcgc gcaaattgcg cgatagcccc tggtggagga agaaaaaaca
      421 atccggtgtc tgctactatt gcagaaagaa attcaagccc gctgatctga ccatggacca
      481 tttgattccg ctcgctcgcg gcggcagatc ggtcaaagaa aacctggttc catgttgcaa
      541 agactgtaac aacaaaaaac gatattacat tcccgctgaa tggaacgatt acctcaaaag
      601 ccttcgagga gaagagccgc cccccgagaa ccaggagatt tctacttgag gcaggctttc
      661 ttttgcgcat ggatcgcatt tgggctgtta ggtgcctgca aatccactta tcccggtggc
      721 attccggcca gtgcagaccc ggcttccgcg cttactgcct gtagcacgtc aggccttgag
      781 accactggtg taaagagagt cgctgtgttt gtgttcgatc caggcggaaa agaagaagcc
      841 ttgcgcatga aggttctgga cgcatatgct gccggattct tacgggacaa aaggtttcgt
      901 cctgtggaaa gagagaagct cgggacgatt cttcgcgaac agggtttatc ttcagccggg
      961 ttattgaccg gggaggatgc agcaaagatg ggcggggtgt tgccagtaga ctggattgtt
     1021 tctgctaccg ttatgcgatc ttccggcaaa gttactgtga gtggcagatt cctcgacgcg
     1081 gtgaacggtt ctattctgca ggggtttgat tgcccatttc tagtggattc catagcggca
     1141 gatcagagcg ttcagatttc gcagaacatc acaaatagca ataatacaac taacagcaat
     1201 aacacgacta cgagtagcac gattaccagc aatacaatca atagcaacaa caccacaaac
     1261 agcaacaatc agagtacgaa catcaacata ggtgttctca atgtaacgga tcccactcga
     1321 atcattcaga agaaatgtga ttcagtgcac gcgcctgtga ttgcagctct tcgcaatctt
     1381 acatctcctg agcttgtgga gagggcagcg cgtctggcca tgaagatccg ttttgatatg
     1441 gaatgcggtc agattcattt cctggtgatg gatacgtttg agcgttatgg atttgccccg
     1501 ccggaatacg gagatttttt gattcgcacg atggacggga tagacgacgt ttccgacgat
     1561 tctcgcactc aatcgatcct tcgctacctg gccgccacgg atggcagggt cgatcaggcg
     1621 gaatggaagt caggcttttc tgctctcatg cgagcgcgaa ctggtagcgt gaccgggtat
     1681 ttgatctatt tactaaacca tcggcgtgag ggaatgatcg cagtggatcg tgcgcgagag
     1741 gtgctctccg cggcagatgc agggaggctg ggaaggcctg tgcccgcaaa gggaggtcag
     1801 attctttatt ctgtcttatc tgctttgaag tttgatagcc ctgccggatc cggacccttt
     1861 gtgtctgttc tcagtcaaaa ctcagaaaaa tatgctgcct accagtacga gagactgagt
     1921 ttcgcatcgc ttgtgtctct ggtgactgct tactatcgct acgcagatga agatggtaga
     1981 accgttatgc ggcaatcgct ggctcgattt tttgcaggac agaaaaatca atcagatcga
     2041 gcgcatgagc atctcttcta tgacatcgcg tatggcctgg aacgcgaagc gaatggagaa
     2101 gacggagaga gtcctgataa gagaaaccgt gccgccaggg aactgcaaag tgctctcatc
     2161 gacttgcatg atcctatgtg ccgggctttc acactgtatg atcgcccaac tctctccacc
     2221 cgggtggatc aaattcattt cttgctgcga aatggcgctg cttgtccggg actcccaact
     2281 gtctctgaac tgatcagcaa ggtggaggcg ggggactggg atgaaaaggc ttctgcattt
     2341 cggactctcg tggaaatgcg aggcaacgcg aagcaggcag aagcagtggc cattcgttac
     2401 ttcaagatag aaggcatgag tcgatccagt gaaattcgag cctactgcgc acaaattctt
     2461 ggctataccc gaacaacgaa tccagaggca caccagctac tgattgccgg cgcaggcgat
     2521 tttgattctc ccgttcagga ctcctctgaa aaagccctgg gtcgactcgg gttgctcgtc
     2581 aagcctgcca ttcttcgata cttgatggtt cctcgaacca gacaggatca ggtgcgcgaa
     2641 atcaggagga ggatggccat ggtgcagaca ctgggaagaa tgggcagaga cgcaagtgat
     2701 gccattcccg ttttgaatca aattgcggcc acggatgtgg actcgtatct ccagagaatg
     2761 gcaaagactg cgattgtcaa tattcaggaa aacagaactg aatagcgcct aacggttcta
     2821 tttcttgatt tgctcaatcc aatcgttcac tgtatggccg ctcgccgccc gttcaaaatg
     2881 gaatcctact tttgcttcaa tcaggtcatt gttcaagaaa ggcaacccca gcatggtcca
     2941 gagtcggatg gcagttcctt cgaagaagtt gtgtagagtt gtggagaatt gatcctgtct
     3001 tccattcaag aagtcttcta gatcgttggc ccagtagatt tcatctggct ttgctttagt
     3061 tcgcccggcg gacccttctt gtttgttctg ttcctttagt tccctgaacg tcatgtccgc
     3121 gaatcggatc gtgtaatctt catggcgatg cgaagaggaa gttccgtata gaatgcgaat
     3181 ggtgtaggat ccgcgggcca aaagcaaagc cttgagcggt tcctctctgt ggtatgccat
     3241 gtgaggaaca aatctgttgt tgagtatgtg aagaatcctt tgcttttgca aagaatgatc
     3301 tcgcagatcg tcgaacaacg gtccctgtgg ccgcggggcg ggagccggtg cggccgggtc
     3361 ctgggtgagc ctggtctgtt ccactttgat ctgcgatttt gatttcttga tcgtgcgtga
     3421 gtcaatctgc tccccggctt cgagagtcat caatttcagg tcatatccag tttcgcgcgc
     3481 tgcctggact tcgagtgccg ttacatctgc cagggggaag gggttagaaa gagggagaaa
     3541 ttcgcgtgag agatctacgg ggaaaatcat tccctgaccg gtgatcagtt tcaggcttcc
     3601 aggcctgaac ggacagggca actccaattc cgcataacgt gcgtattccc agtaaagttc
     3661 gtcctttcgc cttcttctat aggcagtgag tttctgtttt ctttcctgcg gagtctttgc
     3721 atcatacgag gcgtagagtg cttccagttc attcctcgtg gatacaaata cgcgttcggt
     3781 ttctcccatc gctcgatcca cagctccgtg tgcctcctgc gagtccggaa gcgcaatgtc
     3841 tgcctcgaaa aatacggatt ctttcttact tcgaatcacc agaggcatca cgtcttcttc
     3901 gttggtgaga taaggaaccg caaatgcaaa tccagtccag gacagtccgc aaatgttcga
     3961 cgttttgttg tgtctgagga cttcaatgga tgcgccgggt agaaatttag aaagaagagg
     4021 caccagaaac tcgattgttt cgggcacgac aagaacgggc gtctgtttct ggtagagtgc
     4081 agttaaagtg tacgggtcca gatggtcgca atgagagtgc gaaatataaa ccgcgtccaa
     4141 ttttccgatg ctaccgaggt ccacacgagg gttgcgctgc atgaaatcgc caaaggcgaa
     4201 atccgaaagc cacgcatcga acaggattct aaccgggccg gtagatccct ctatttctag
     4261 aaggacttcc gaatgcccgg gagaagccag aatcacgctt ttttcatttc agtgcgaaat
     4321 acctgcaggt aaaagaacac tgccatcatg attggaattg cggccaggaa tccaatgtct
     4381 ccgccgtaaa gggcgatcat cagtgctcca atgaatagtc tatgaatctc aagtatatgt
     4441 gcccatctct ttgactcaaa aatgccgccc agagtcaaaa gagacagcgc cacaaatccg
     4501 ccgtagaata gcttcgcgcc cagaagcatg gttggactca tgaagaagaa gtaggacgcg
     4561 aatccgagtg tgaaaaggaa attcacaaat gtatagattt caagagctgt gctgagtttg
     4621 gtgcgatact tggccggata acgggctgga acacgattct tcattccttc aggtgagtaa
     4681 gccggatctg ccatgaacgc atcgtactcg agtcccgttg gaagccagcc aggtcctttg
     4741 aaccagagca aaatcttatc cagaaagcgc ggagctttga tggatttttg aaaaagttcg
     4801 aggaagtaat gaacctgtgc ccagattgga ttccagctgg ccagaggttt gatggtcccg
     4861 tagtgcactt cttctgtttc ttcttgaaac gttccgaaca tcttgtccca gatgatcaac
     4921 gttccggcgt agttcttgtc tatgtagatt ggatttttgc cgtgatgcac tctatgatga
     4981 gaaggagtgt tcatgaagat ttccaggaaa ccaagtttgc caactgcgcg agtgtgaatc
     5041 cagaactgat agattgtgtt gaactgggaa tgaaccagga agatcagagg tggaattccg
     5101 accagtgcaa gcggaacata aaaccaaatg gagaagaaac tttgaaaagc gccctggcgt
     5161 agagccacag tcaggttgta ttcttcgctt tgatggtggg gaagatgcga tccccagaag
     5221 actgcagatt catgagcaat gcgatggaac cagtagtaga aaaaatccac gccaaagaag
     5281 catgcaaccc aggcaatcac tgacttttct gagatttcaa gaagccgcca gttctgatac
     5341 acgtaaacgt agagaccgac cgtaatgatt ttgccgaata ccccgacgac ttgagaaaga
     5401 attccagcgc tcaagtcgtt tacagagtcg tggtagtcat agtatccacc tttcttcagg
     5461 cgtgagacga tgatctccac cgcgatgaga aacaagaata ccggaattgc cattgtgatg
     5521 tagtcaggcg ccatgcgaaa aatccccttg ttatgtctgg tttaatgata aaaagtaagg
     5581 ccgcaagcgc gaaaatttag atttttgaat ctcaaatgat tgcgcgaatg attcgcctca
     5641 gttcagctga caaagccgca aacgcataaa tatgaaaact gtagtaatcg atggtgtaac
     5701 tgttcagctt gcaagtccag atttgatcga ccagaactgg gtcggaaaca tggacgttct
     5761 caatcaattg atggcgtgct gggttctcgt tcatccaagt gacctgcctc tcaatccaag
     5821 gttgattggc cggccgggag taggaaaaac tactctggcc tgtgcagccg cgcgtgtcct
     5881 ggataaacca acctatatct ttcaggctac catggatacc aggccggaag atctgctcat
     5941 cactccggtc atttcagaat caggaaaaat caaatacgtg gcttccgcca ttgtcaccgc
     6001 catgctagaa ggtggaattg ctatactcga tgaaggcaat agaatggggg aaaagtcctg
     6061 ggcgtccctg gcacctttgc tcgaccacag acgttacatc gagtccatcg ttgctggcat
     6121 caagatcaag gcaagtcctg agtttagatt ctgcgctaca atgaatgaag atgcttccac
     6181 ttacgaaatc ccggaataca tccagagcag actccagccg cagatctaca tagactttgc
     6241 agacgcggat gaagagctag agattctg
//
LOCUS       DAOQJL010000181         6261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7032_length_6261_cov_4.044151, whole genome shotgun sequence.
ACCESSION   DAOQJL010000181 DAOQJL010000000
VERSION     DAOQJL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7032_length_6261_cov_4.044151"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLG78_09370"
     CDS             complement(<1..879)
                     /locus_tag="PLG78_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23366.1"
                     /translation="MSVRNIKPKELSDIIADPAELSKGIRIRDDGGLQHPARFENKLF
                     ADAKGSGASPYKVQIIYDEAENKFKGRCSCMAARSRPFCKHAVALLAAWSSAPEAFAV
                     SEGPPQGTPGETKKAKVKTSKVNTKNLMAEGVAQAATLVGELAISGLSGLSRQRLEQV
                     ESLGANLRESKLRRLSARLQTLAAQMSKASGIEKSAVTLAQLYSDAVLTVRKLEKHLA
                     GEELEAKYVEELIGKNWGKKDRKSQSGLDLLEYAYIHHTTADDFVIRESRFVDLQSGA
                     HYSEKQILPAFLAKKTQ"
     gene            complement(876..2054)
                     /locus_tag="PLG78_09375"
     CDS             complement(876..2054)
                     /locus_tag="PLG78_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE23367.1"
                     /translation="MKQDAKEAILRWRLALGPGAEKTSSEFNLREGLGLPEERLSDLD
                     DALSFVYREKGAGGTGASAPYIPKWLGHLRDFFRQEVVAFVQKEAIEKRGLTQLLFEP
                     ETLPFLEKNVDLVSTLISARGLIPEKAKEIARQIVREVVEELRKKLESQTRTAILGAV
                     RRNNPSPLKVLRNLDWKKTIRKNLHGWNSETKRLVPEEIYFWSNQRRQHEWDVVLLVD
                     QSGSMATSVVYSSVMAAIFASLDVLKTKLVFFDTEIVDMTSYLTDPVEVLFSTQLGGG
                     TDINRAVAYAQANLIERPEKTLFILVTDLFEGGNQSELVMRMRQIVDGRSKTMVLLAL
                     SDRGQPSYDHGLATTLTEMGVHCFGCTPALLIGVVERIMKNQDVTEYVAAYQKAEKES
                     "
     gene            complement(2051..4342)
                     /locus_tag="PLG78_09380"
     CDS             complement(2051..4342)
                     /locus_tag="PLG78_09380"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNE23368.1"
                     /translation="MASKKKQPEIVLPDVSFFGIRHHSPRSSSALFALLEKMRPECIL
                     VEGPTDARPLFEAVAASDTLPPVAILGYRTDDQVGSSFYPLASYSPEYVAIRWAFANK
                     VDFDFIDIPFEQSIAYDRHQKEDPSEDMEPENNSALFYKTLADRFGCRSFEEFWEAQF
                     EGPSYDPDTFRAVIVAYSDVSRQFGADLDFHRARDAFMAARIQDKIQGGANPSRTLVV
                     AGAAHVAAFMAKDVAPEKIDLLPAACPVAVTLIPYSFTRLAEQTGYGAGNRAPRYYER
                     AYQRGCDFKAASLEMLIEFCSHLRLRGFAVSLADTIEAYRLANMLAQMRNKSAPGLDE
                     VRDAAAATLCRGELSHIDGFLWSTVVGSAVGKVGAAVAKNSLQQEFWNEVRKFRLPES
                     DNPESFVLKLNDPHHVSASVFLHRLRISDVPYGSFIGKDEREAAGGVDALSRVRETWQ
                     AQWTPATELALAERVVLGDSFERVCARRLRDSLSSAPRTAEAAQILLEAVVADIDGIV
                     SSALEVCNKSAANDGDVISLANACRTLSGLVSYGTSRKSMEGAKAAIEALCSQTFSRV
                     SLRIPDACKTDDDGAPSIQNAILAIHEIALAQKLVDKQLWFETARQLIRDYTIHPLCA
                     GSIAGLLYVAREISDKEAQEIIEQRLTFRSQPAQAAGFVAGFLEVNALIIVRDAGIVE
                     KLNEFILELSADEFVNALPMLRRAFVQLGATERRYLIENILKLTGASPAESLAMLKEK
                     DKEKLKTVSKEMGKIMKDLDDLL"
     gene            complement(4345..5418)
                     /locus_tag="PLG78_09385"
     CDS             complement(4345..5418)
                     /locus_tag="PLG78_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE23369.1"
                     /translation="MRLSAESKFEKEIQALIAGDKSEKPPGWAMTPRAAETYLMGSDK
                     PVGGVKITPKYIGDASIIQVAIATLASDRALMLTGEPGTAKSWISEHLSAAISGTSAY
                     VIQGTAGTSEEHIKYGWNYAMLLAEGPSHKSLVASPILRGMKEGKFARFEEITRTSSE
                     IQDALISILSEKQIAIPELGEMIHAKRGFNIIATANTRDRGVNEMSAALKRRFNFVNI
                     PIVDDLEQEIQIVLKRESELRSDYSVGAQPPEELGKVLVTVFQELRKGITRDGKIKIK
                     SPNSVLSTAEAISVLFNGGILAQSFGSGKVTGAEIARALVGAVAKESQEDLKVLKEYC
                     ETVVKTRTGPWKEFYKAATEKLN"
     gene            6017..>6261
                     /locus_tag="PLG78_09390"
     CDS             6017..>6261
                     /locus_tag="PLG78_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23370.1"
                     /translation="MLAIFPGASYAYSKVEDLPENGNKTGLVREGSTTDLAPSHNSGK
                     LTSANGGGRAPSYLSPGRIYRDMGLRYKMIVLILGVVS"
BASE COUNT         1371 a         1620 c         1546 g         1724 t
ORIGIN      
        1 ctgagttttt tttgcaagga atgcaggcag aatttgtttc tcggagtagt gcgccccact
       61 ctgaagatca acaaaacgac tctctcgaat tacgaaatca tctgcagtcg tatgatggat
      121 gtaggcgtac tccagtaggt ccagtccgct ctgcgatttt cgatctttct ttccccagtt
      181 ctttccaatc agctcttcta cgtattttgc ttctagctct tctcccgcga gatgcttttc
      241 taatttgcga accgtgagaa ctgcatctga atagagttga gccagggtca cagcggattt
      301 ctctatgcct gacgctttag acatttgcgc cgcgagggtt tgcaggcgtg cagaaagtct
      361 acgaagtttt gactcgcgca ggtttgcgcc aaggctctcc acctgttcca atctctggcg
      421 agacaatcca gacagaccac tgatcgcaag ttcgccaacg agtgtggctg cctgcgcaac
      481 cccttccgcc atcagatttt tcgtgtttac cttgctggtt tttacttttg cctttttagt
      541 ttcgccgggc gtaccctgtg gcggtccctc acttacagca aaggcctccg gagcagagga
      601 ccacgccgca agaagtgcaa ctgcatgttt gcagaacggt cgcgatcgtg ctgccatgca
      661 tgaacagcgt cctttgaatt tgttttctgc ttcatcataa atgatttgaa ctttgtaagg
      721 cgacgcgccg gaaccttttg cgtctgcaaa tagtttgttc tcaaaacggg ccggatgctg
      781 gaggccgcca tcgtctctaa tccgaattcc tttgctcagc tctgctggat ctgcaatgat
      841 gtccgaaagc tctttgggtt tgatgttgcg aacgctcatg attctttctc cgccttctga
      901 taagcagcta catactctgt gacgtcctga tttttcatga ttcgctccac aactccaatg
      961 agtagagccg gagtgcatcc aaaacaatga acacccattt ccgtgagggt tgttgccagt
     1021 ccgtggtcgt aggaaggttg tcccctgtct gacaatgcga ggagtaccat agtcttcgat
     1081 ctgccgtcta caatctgtct cattcgcatt acgagttctg attgattgcc gccttcgaat
     1141 agatcagtca cgagaatgaa aagtgtcttt tctggccttt ctataagatt cgcctgcgcg
     1201 taggcaaccg ctcgattgat gtccgtgcct ccgccgagct gtgttgagaa taaaacttca
     1261 acgggatccg tcagatagga agtcatgtcc acgatttccg tatcaaagaa gaccagcttc
     1321 gtttttaaga catcaaggga tgcaaatata gccgccatca cagaactata gaccacactc
     1381 gttgccatgg atcctgattg gtctacgagc aacacgacat cccattcatg ctgcctgcgt
     1441 tgattggacc agaagtagat ttcttccgga accaatcgct ttgtttccga attccatccg
     1501 tgtagatttt tgcgaatcgt cttcttccag tctagatttc gcagcacctt tagtggactt
     1561 ggattgtttc ttcttaccgc cccgagtatg gcagtgcgag tctgcgattc caattttttg
     1621 cgcagctcct ctacaacctc tcgaacgatt tgtcgcgcga tctcttttgc cttttccgga
     1681 atcagtccgc gggcgctgat caaagtggaa acaaggtcaa cgttcttttc cagaaatggg
     1741 agtgtttctg gctcaaagag tagctgcgta agcccgcgtt tttcaatcgc ttctttctga
     1801 acaaaagcca ctacttcctg cctgaaaaaa tcgcgcaggt ggcccaacca ttttggaata
     1861 tatggcgccg acgcgcccgt gccaccggcc cctttctctc gataaacgaa tgaaagcgca
     1921 tcgtccagat ccgatagtcg ctcttctggc aacccgagtc cctctcgcag attgaattcg
     1981 ctggatgttt tttctgcgcc tggccccagg gcgagtcgcc atctgagtat cgcttccttt
     2041 gcgtcttgct tcatagcaaa tcatccaggt ctttcatgat tttgcccatt tctttgctga
     2101 ccgttttaag tttctctttg tccttttcct tcagcatggc aagcgattcc gccggagaag
     2161 cacctgttag tttcaaaatg ttctcaatga gatagcgacg ttccgtagca cccagttgga
     2221 caaatgctct gcgcaacatg ggcagtgcgt ttacgaattc atctgcagac aattcgagaa
     2281 tgaattcatt tagtttttca acaattccgg cgtcgcgaac aatgatgaga gcgtttactt
     2341 caaggaatcc tgccacaaac ccggctgcct gggcaggttg cgaccgaaac gttaggcgct
     2401 gttctatgat ctcctgtgct tctttgtctg aaatttctct ggcaacgtag agtaagcctg
     2461 cgatcgaacc cgcgcagagt ggatgaattg tgtagtcgcg gatcagctga cgcgctgttt
     2521 caaaccagag ttgcttatcc accaattttt gcgccagagc aatctcgtga attgcgagga
     2581 tcgcgttctg gatggacggt gctccgtcgt catctgtctt acatgcatct ggaattctga
     2641 ggcttacgcg cgaaaatgtc tgcgagcaca aagcttcgat agctgcttta gctccttcca
     2701 tggatttccg tgacgttcca tagcttacca ggccagagag agttctacaa gcattggcga
     2761 gcgaaataac atctccgtcg ttagccgcgg atttgttgca cacttccagc gcactcgata
     2821 cgattccgtc aatatccgca acaactgctt ctaacaaaat ctgggccgcc tccgcagtcc
     2881 tgggcgccga tgaaagtgaa tcgcgcaaac gccgcgcgca tacgcgttcg aacgaatctc
     2941 ccagcacaac tcgctccgca agggccagtt ctgttgccgg agtccactgt gcctgccacg
     3001 tttctctgac gcgcgacaat gcatctacgc cgcccgctgc ttccctttca tcttttccaa
     3061 tgaacgatcc gtacggaaca tcgctgattc gaagcctgtg gagaaataca gatgcggata
     3121 catggtgggg atcattgagt tttagaacaa agctctctgg attgtcgctt tccgggagtc
     3181 tgaactttcg cacttcgttc cagaattctt gctggagaga attttttgcg accgctgcac
     3241 ctactttccc gacagcgctg ccaaccacgg tagaccatag gaatccatca atatgactga
     3301 gttcaccgcg gcatagagtc gcggcggccg catcgcgtac ctcatctagc ccgggggcag
     3361 acttgtttcg catttgagcg agcatgtttg ccaggcgata cgcttcgatc gtatctgcca
     3421 gggacacagc gaatccacgc aagcgaaggt ggctgcaaaa ttcgatcagc atctcaaggc
     3481 tggccgcttt aaaatcgcag ccgcgctggt aggctctttc atagtacctg ggcgctctat
     3541 ttcccgcacc gtaccctgtt tgttctgcaa gacgcgtgaa gctgtagggg atcaaggtga
     3601 ctgcgacggg gcaggctgcg ggaagcagat cgattttctc cggcgctacg tcttttgcca
     3661 taaatgcagc aacgtgagcc gctcctgcca cgacgagtgt tctggatggg tttgcgccgc
     3721 cctggatttt gtcctggatt ctcgcggcca tgaaagcatc gcgtgcgcga tgaaaatcaa
     3781 gatccgctcc gaattgccgg ctaacgtctg aatatgcgac tatgactgcg cgaaacgtgt
     3841 ccggatcata ggatggtcct tcaaactggg cttcccagaa ctcttcaaac gatctacatc
     3901 caaaacgatc tgcaagtgtt ttgtagaata aagcagaatt gttttctggc tccatgtctt
     3961 cagatggatc ttctttttga tgtcgatcgt aggcaatgga ttgttcgaat ggaatgtcga
     4021 tgaagtcaaa gtcaactttg ttggcgaacg cccagcgaat tgccacatac tcgggagagt
     4081 aagaggcgag cgggtaaaaa ctcgagccaa cctgatcatc tgttctatat ccaagaattg
     4141 caacgggcgg caaagtatca ctggcggcca ctgcttcgaa caaaggtctc gcatccgtcg
     4201 gtccctctac cagaatgcat tcggggcgca tcttttcaag gagtgcaaag agtgcgctcg
     4261 agctgcgcgg agaatggtgc cgaattccaa aaaatgaaac gtccggcagc actatttcag
     4321 gctgcttctt ttttgaggcc atgatcagtt caatttttcg gttgcggctt tatagaattc
     4381 cttccaggga ccagtccgcg tcttgacgac tgtttcgcag tactctttca gcaccttcag
     4441 gtcttcctga gattctttag cgacagctcc gaccagggcg cgcgcaattt ctgcgcccgt
     4501 gaccttgccg gatccaaaac tctgcgcaag aatgccgcca ttgaataaaa cagaaattgc
     4561 ttctgctgta gacagaactg aatttggtga tttgattttt atttttccgt ctctagtgat
     4621 gcctttgcgc agctcttgaa agactgttac aagcactttg ccgagttctt cagggggctg
     4681 ggctcccacg gaataatcgg atctcagttc ggactctcgt tttagaacaa tttggatttc
     4741 ctgctccagg tcatctacaa taggaatgtt tacgaaattg aaacgtcgct tcaaggccgc
     4801 ggacatttca ttgacgccgc gatctcttgt gttggctgtt gcgatgatat tgaatccgcg
     4861 tttagcatgg atcatttccc ccagctcggg gatggcgatt tgcttttctg aaagaatcga
     4921 aatcagagcg tcctgaatct cggaactggt ccgagtgatt tcctcaaagc gtgcgaactt
     4981 tccttctttc attccgcgca agattggaga cgcaaccagg gatttgtgcg aaggcccttc
     5041 tgccaggagc attgcgtagt tccagccata cttgatgtgt tcttcgctgg tgcctgccgt
     5101 gccttgaatt acgtatgcgc tcgtccctga gatcgcggca gacaaatgct ctgaaatcca
     5161 gcttttcgca gtacccggct ctccggtcaa catcagggcg cggtcggagg caagggttgc
     5221 gatcgctacc tggatgatgg aagcgtcccc aatgtatttg ggagtgattt tgaccccacc
     5281 gactggtttg tcgcttccca tcaaatacgt ctcggcggcc cgcggcgtca tggcccaccc
     5341 tggaggcttt tcgcttttgt cccctgcaat gagtgcctgg atctctttct cgaattttga
     5401 ttccgctgat aagcgcactg catccatcta taaactctcc gctgggcaat cggcatatag
     5461 atgaggcgaa gtggcaattc aaaaatcggc gtttctggtt ccggctggct tccgggctct
     5521 ggaagtcagg atcgtttttg cggtgcgaat ctggaatcaa cgacacgaaa ttggtaatga
     5581 acaacacttt caaacgttaa gcgatctttc ttgaaattgt tttatgtttt tatatagttt
     5641 agatgtggtt attcaatgaa aatgcattga cccgttcttg cggcgttccg gttctggaga
     5701 tgtactgatt gctgattcgc ggggcgtcag aaggagttta caatgttcaa acgcttcctg
     5761 atctctcttt cggttttgct cgttgtgttt tgcgcggaaa aaaaggagga ggataattcc
     5821 agcctgcttc tactcgggtt gctaggggcc ggaagggcaa gttctacgag ctcttcggcg
     5881 acttgcggtg gtgttgccct cacttccgcc actgcgttga cagacgatgc tgccgcagtg
     5941 acaagcagta cacttcccgg aatctacgcc gcctaacgtt ggacgttttg aacgaaaagt
     6001 cacaaatttt tgaagagtgc ttgctatttt tcccggtgcg agttatgcct actcaaaagt
     6061 ggaagatcta ccagaaaacg ggaacaagac gggcttggtg cgcgaaggct ccacgacgga
     6121 tctggctcct tcgcataact ctggtaagct gacctcggca aatgggggtg ggcgcgcccc
     6181 ttcgtatctt tcccccggtc gcatctatcg cgacatgggt ttgcggtaca aaatgatcgt
     6241 cttgatcctg ggggtggtat c
//
LOCUS       DAOQJL010000182         6240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7072_length_6240_cov_4.920776, whole genome shotgun sequence.
ACCESSION   DAOQJL010000182 DAOQJL010000000
VERSION     DAOQJL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7072_length_6240_cov_4.920776"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLG78_09395"
     CDS             <1..929
                     /locus_tag="PLG78_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNE23371.1"
                     /translation="AIARCDLDIPIVLGQTLFRPPFAIGHEFVGSIESVSEDLAGQFQ
                     KGDRVAVAFQISCGTCPMCTGGLSKNCTTVTPAANYGMAPAAKEFGGALADFVKVPFA
                     SSMLLRLPPNVPAASVASLSDNIAEAWKLAGSVLSNRPSSTVLILGGVASSIGLYTAM
                     LAKIMTKGSVLYMDTDPARVLHAERLGIQAEHVKEFPKSRGKFDLVCECAASEAGWTC
                     ATRSVAPAGVLTTASIFWTNKLEIPFLEFYNGGVELRISRVDSREMMEKMLTLISDGT
                     YDPGLVTTSTASLADAKEAWMEPATKLVITNE"
     gene            complement(932..1396)
                     /locus_tag="PLG78_09400"
     CDS             complement(932..1396)
                     /locus_tag="PLG78_09400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE23372.1"
                     /translation="MYPKIRITLPAGTIECELYNDVAPRHAERILTLAGKGFYDGILF
                     HRIVPDFVAQAGDPKTKNGLNQPGVGAGGSDLPDLPLEVSPLQKNTRGSLAMARTQNP
                     NSANSQFYIVLKDAPHLNMQYTVFGRVLENGMDVVDKIKMGDAIIKMEEIKG"
     gene            complement(1479..2534)
                     /locus_tag="PLG78_09405"
     CDS             complement(1479..2534)
                     /locus_tag="PLG78_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE23373.1"
                     /translation="MSGILRIAYGALAVVCSAGLLETTAGAFASRVDPVTALDPVTRY
                     AYKPGLDLVSTAEEYRITFRTDSMGLRVCGSGEVGREPPAKGGFYFIGDSFTEGLGVD
                     CEKTFAAAVARSKRKDFFNAGMHGGAPPFYVLRNQKFLKRLTPEAVFVQIYDNDATDM
                     DAMSEFISLGQDVRVKPLSEKESLRIHLTEHSSFLSALRIRLKENHSPWFFRAGTAPA
                     ELPPGTDASKNSDSFGFYGKTKMESPWSNRLEMLAASMDRMIQDLRVLAPDARLVLIH
                     IPDRTIYQYSLVSQEQNAVRAMVRLAAARSGAIFVDGQVALSRDPGTKYFPGNGHINA
                     DGHAAIARAILVQLGTR"
     gene            complement(2518..3054)
                     /gene="lptE"
                     /locus_tag="PLG78_09410"
     CDS             complement(2518..3054)
                     /gene="lptE"
                     /locus_tag="PLG78_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNE23374.1"
                     /translation="MTIAQRVCILCVIAAQGTCRLLQHTDLSDRDPATRTIFVHNFSN
                     ETFQPDVQAEITDWLKKELSRRDNFKVVSNREDARIWVYGEVVVFSKEGRMYDNFRNP
                     TRYEMLTTVHIKMRENPARSTGGPLLDKSGEVTQTIQFSEKEGYKEPEFQARQRMLRI
                     LASKISDALESEYLEWHP"
     gene            complement(3051..4136)
                     /locus_tag="PLG78_09415"
     CDS             complement(3051..4136)
                     /locus_tag="PLG78_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNE23375.1"
                     /translation="MNRRLGIILGVGSLLILGLLLLFGPGTQKDKPDAIFWKKDFDAI
                     EYVSAGKNRATSLRLVRKAGLIRDEFTVEGSGKSARRGNYNAKNIFTDFSKPRNAGQY
                     KLTPERVEELGFTAESPQVILYEKIGSAPLRLTVGKKNQTGNTFVRCDDPSFKDQVLL
                     MPSYLFERFDKPIKEFREQRFLVYSTDSFTEELEVTIDGGGPIKSYWMKQEKYKKDGI
                     ELPRWRDRQGTEIPLNQASALENAVREIQILEYRDADGLPSAEDAWKRAGKDTTVVKV
                     KVKGQDETIIRLRSSYVIQNGKDSLITVQSTVEPGTDFVRANVEHDLASKIDQIVESL
                     QKKAEVEKRNHEQKDQPNASPAVEPTR"
     gene            complement(4133..6046)
                     /locus_tag="PLG78_09420"
     CDS             complement(4133..6046)
                     /locus_tag="PLG78_09420"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNE23376.1"
                     /translation="MKNFIDILSDVYSRTGVWIQTVATLTLFTSFSFLDGPTGWVFFA
                     LSAIVIVSDPVVLVLSARSRGARVQSGYLATVVLIVVAVAAYAIRTKLNLTLDTVADA
                     DDAWLTRLRRLLLALFLFSYAGFFVYRLFICIGENATRIQGSADIKRRQMLQKSLISI
                     FAALPLFVLANYLTTVRNPTIDMSPGYYSFGEASRTILKSVPVDVEATVFLPEMQAMK
                     DSSQSKTQPELFRIAEDVRVMADQLPVINGHFKLKYRNADMDLVASQDFGSVNNGTFV
                     FRVMKQGLTTGDNKPYIERRVYVFNESDMERLEREIVRAAIQVSSPEKNVYFTAANGE
                     RWDVPGRVRSPGAIETMKDALRFMNFTLKPLDSQNSWPGPIPKDADALVIAGPTVSFS
                     PEARTAVLDYLKRGGKVLASINPAGPEDMSWLLEAADSHLRFKNAFLSSEAVWPGIPI
                     TDSLETHRITENLSVAGRAFIAFPECGYFAEGSVPSAQPQILQENNQKMKQTIFLYTK
                     FNTVVDTNRDGRKEPGEESGRFPLGVAFEYPDHKGARLVVYSGIGWMTELGLRFPIDQ
                     RNIILAQDSLSFLMENPIAAGLVPEQRKTRSIPVTEGLKFRNFILGVVAFPLSTVLLT
                     LGGVWLYRRRRKK"
     gene            complement(6043..>6240)
                     /locus_tag="PLG78_09425"
     CDS             complement(6043..>6240)
                     /locus_tag="PLG78_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23377.1"
                     /translation="GGVLPSPAKEIVTHFHGLSQFAPFARGVISLKACVFFISLTIFF
                     LFLTVRVLESRRWRLQAGGNG"
BASE COUNT         1542 a         1634 c         1501 g         1563 t
ORIGIN      
        1 tggcgattgc tcgctgtgac cttgacattc ccattgtact cgggcagaca ttgtttcgcc
       61 ctcctttcgc catcggacac gaattcgtag gatctattga atccgtttct gaggacctgg
      121 ctggccagtt tcaaaaggga gatcgagtgg ccgttgcatt ccagatctca tgcggcacgt
      181 gccccatgtg caccggaggc ctatccaaga actgcactac tgttacaccg gcggccaact
      241 acggaatggc acctgcggca aaagaatttg gaggagccct cgctgatttt gtaaaggtac
      301 ctttcgccag ttcgatgctt ctgagattgc cgcctaacgt tcctgcggcg tctgttgcaa
      361 gcctgagcga caatattgca gaagcgtgga agctggcagg ctcagtgctg agcaatcgtc
      421 catcttctac agttctgatt ctaggcggag tggcttcgag cattggcctc tatactgcta
      481 tgcttgcgaa aatcatgaca aaaggatctg ttctctatat ggacaccgat ccggcgcgag
      541 tccttcatgc agagcgactt ggaattcaag cagagcacgt caaagagttc ccaaaatcgc
      601 gcggaaagtt tgacctcgtc tgcgaatgcg ctgcaagtga ggcaggctgg acttgcgcca
      661 caagatccgt agctccggcg ggtgtgctga caacggcgtc catcttctgg accaacaagc
      721 ttgaaatccc gttcctcgaa ttttataatg gtggagtgga gctacgaatc agccgcgtgg
      781 attcaagaga gatgatggag aaaatgttga ctctgatatc tgatggaacc tatgaccccg
      841 gcctggttac aaccagcaca gcctctctcg cggatgcgaa agaggcctgg atggagcctg
      901 cgaccaagct ggtgatcaca aatgaataga tctatccttt gatctcttcc atcttgatga
      961 tggcgtctcc catcttgatt ttgtccacga cgtccatacc gttttcgagg acgcgcccga
     1021 atacggtgta ctgcatattc aggtgcggcg cgtctttcaa tactatataa aattgactat
     1081 tagcggaatt tggattctgt gttcgagcca tggccagact gccacgcgtg ttcttctgaa
     1141 gggggcttac ctctaaaggc agatcaggaa gatccgaacc acctgcaccc acacccggtt
     1201 gattcaaccc attctttgtt tttggatctc cggcctgggc tacgaaatcg ggaacaatgc
     1261 gatgaaataa aatcccatcg tagaagccct tgcctgccag agtgagaatg cgttccgcgt
     1321 gcctgggcgc tacatcgttg tagagttcgc actcaatggt tcctgcaggt agagtaattc
     1381 gaatttttgg atacatggac tgaagatttc tgaacccttt gtcccgtccg ccagaaaaac
     1441 aatttctcag actacgcgaa aaggtccgac aagatcgcct aacgcgtgcc cagttgcact
     1501 agaatcgcac gtgcgatcgc cgcatgacca tccgcattga tatggccatt gccagggaaa
     1561 tactttgtac ctgggtcgcg cgatagcgcg acctgaccat ccacgaaaat agcgcctgat
     1621 ctggctgccg cgagccttac catggcccgc actgcatttt gctcctggct tacaagcgaa
     1681 tactggtaga tggttctgtc tggaatatgg atgagcacaa gccgggcatc tggtgcaaga
     1741 acgcgcaaat cctggatcat ccgatccata gatgcagcca gcatttccag gcgattcgac
     1801 cacgggcttt ccatctttgt ctttccgtaa aaaccaaacg aatccgaatt cttactcgcg
     1861 tccgttcctg gaggcagctc ggccggagct gtccccgcgc gaaagaacca cggactatgg
     1921 ttttccttca gccgaattcg cagggcagaa agaaacgaag agtgttctgt aagatgaatg
     1981 cgaagcgatt ccttttccga tagaggtttg actcgcacat cttgaccgag ggagatgaat
     2041 tctgacatcg catccatatc tgtcgcatcg ttatcataaa tctgaacaaa gactgcttcc
     2101 ggtgtgaggc gtttcaaaaa cttctggttt ctcaagacat agaaaggagg tgcccctcca
     2161 tgcatgccgg cgttaaagaa gtctttgcgt ttgctccgcg ctactgcggc tgcgaatgtt
     2221 ttctcgcagt ctacgcccag tccttccgta aacgaatcac ctataaaata gaagccgccc
     2281 tttgcgggag gctctcgacc cacttcgccg gaaccacaca cccttagacc catcgagtcc
     2341 gtcctgaacg ttatgcggta ttcctctgcg gttgaaacca gatcgagacc cggtttgtac
     2401 gcgtatcttg ttaccggatc aagggcagta actggatcaa cgcgagaggc aaacgcaccc
     2461 gctgtagttt caagaagacc cgcgctacag acaaccgcca gcgcgccgta ggcgattcta
     2521 aggatgccac tcaagatatt ctgactccag agcatccgag atcttggaag caaggattct
     2581 cagcattctc tgccttgctt gaaactcagg ttctttgtat ccctcttttt ctgaaaactg
     2641 aatggtctgc gtgacttcac cagatttgtc gagcaaaggt ccgccggtgg aacgagcagg
     2701 attttcgcgc atcttgatat gcacggtggt caacatttcg tatcgtgttg ggtttctaaa
     2761 gttgtcatac atcctgccct ctttagaaaa aacaacgacc tccccataaa cccagatacg
     2821 agcatcttct cgattgctca caactttgaa attatccctt ctactgagtt ctttcttgag
     2881 ccagtctgtg atctctgcct gtacgtccgg ctgaaatgtt tcgtttgaga aattgtgtac
     2941 gaatatggtt cgcgtcgccg gatctctgtc agacaaatca gtgtgctgca aaagccgaca
     3001 ggttccctgt gcagcgatca cacatagaat gcagactcgc tgcgcaatcg tcatctggtg
     3061 ggctctacag ccgggcttgc gtttggctga tctttttgct cgtgatttct tttctcaacc
     3121 tcagcttttt tctgcagtga ctcgacgatc tgatctatct tgcttgcaag atcatgctcc
     3181 acgtttgcgc gcacgaaatc tgtgcctggc tccacagtag attgcactgt tataagagag
     3241 tctttgccat tctgaatcac ataactggaa cgaaggcgaa tgattgtttc atcctgccct
     3301 ttgaccttaa ctttaaccac ggttgtgtct tttccagcac gcttccaggc atcttcggca
     3361 gacggaagtc cgtccgcgtc gcgatactcc agaatttgaa tttcgcgcac tgcattctcg
     3421 agagcagaag cctggttaag cgggatttct gttccttgtc gatcgcgcca gcggggcagt
     3481 tcgatgccgt ctttcttgta tttttcctgc ttcatccaat aagacttgat cgggcctcca
     3541 ccgtcaatgg tgacctcaag ttcttctgta aatgaatccg tgctgtagac cagaaaacgc
     3601 tgctcgcgaa actctttgat tggtttgtca aagcgctcaa acaaataaga aggcatcaac
     3661 agcacctggt ctttaaaaga gggatcatcg cagcgaacaa acgtgtttcc ggtttgattc
     3721 ttttttccaa ccgtcaaacg aagcggagca gaacctattt tttcgtacag gatgacttgc
     3781 ggcgattctg cagtgaatcc aagctcttct actctttcgg gagttagttt gtactggcct
     3841 gcgttgcgcg gtttgctaaa atcggtgaag atattttttg cattatagtt tccccgcctg
     3901 gcagatttgc ccgaaccctc aactgtaaac tcgtccctga tcaggcctgc ttttcgaacc
     3961 aggcgcaaag aagttgcacg atttttgccc gcagatacgt attcaattgc atcaaaatct
     4021 tttttccaga agatcgcgtc cggtttgtct ttctgcgtgc cggggccaaa caacaataac
     4081 agcccgagga tcagcagaga tcctacgccc agaatgattc cgagtcgtct gttcatttct
     4141 tacgacgcct ccggtaaagc caaacgccgc ctaacgtcag aaggacagta gaaagcggga
     4201 aagctacaac tcccagaatg aaatttctaa atttgagacc ttccgttaca ggaatactcc
     4261 tggttttcct ctgctccggg acgagccccg cagcaattgg attttccatg aggaaggaga
     4321 gactgtcctg cgccagaatg atgtttctct gatcgatagg gaaccggaga cccagttctg
     4381 tcatccaacc aatgcccgag tagacaacca gtcgcgctcc tttgtgatcc ggatactcaa
     4441 atgcaacacc cagagggaat cgaccggatt cttctccggg ttcctttctt ccatcgcggt
     4501 tcgtatccac taccgtattg aattttgtgt agaggaagat ggtctgcttc atcttctgat
     4561 tgttttcctg tagaatctgt ggctgcgcag aagggacaga accttccgcg aaatatccgc
     4621 attcaggaaa tgcaatgaac gctctacccg ccacagacaa gttctccgtt atgcgatgag
     4681 tctcaagcga atcagtaatc ggaatccccg gccatacagc ttcgctcgaa agaaaagcat
     4741 tcttgaagcg aagatgcgaa tctgccgcct caagcaacca gctcatgtct tccggtcctg
     4801 cgggattgat ggaagcaaga accttgccgc ctcttttcag atagtccaga accgctgtgc
     4861 gcgcttccgg cgaaaaagaa acagtgggcc ctgcaatcac gagtgcatcc gcgtcttttg
     4921 gaatgggacc gggccaggag ttttgagaat caagaggctt cagagtaaag ttcatgaatc
     4981 tgagcgcgtc tttcatggtt tcaatcgcac caggagagcg gactcgccca ggaacatccc
     5041 agcgctcacc gttggccgca gtgaagtaca catttttttc aggtgaggaa acctgaatgg
     5101 cagcgcgcac aatttcacgc tccagccttt ccatatcgct ttcattgaat acgtaaacac
     5161 ggcgctcgat gtatggcttg ttgtcccctg tcgtaagccc ttgcttcatt acgcgaaaca
     5221 caaacgttcc attgttcaca gatccgaagt cctgagaggc gactagatcc atgtccgcgt
     5281 ttctgtactt caatttgaaa tgcccgttga tgactggaag ttgatctgcc atcactcgaa
     5341 cgtcttctgc aatgcgaaag agctcaggct gcgttttgga ttgcgacgag tccttcattg
     5401 cctgcatttc tggaagaaac accgtggctt ctacatccac cggaacactt ttgagaatcg
     5461 tgcgcgatgc ctccccgaag gaatagtacc cgggactcat gtctatggtt gggtttcgca
     5521 cagtggtcag atagtttgcc agaacaaaaa gaggtagtgc ggcgaagatc gaaatcaacg
     5581 atttctgaag catctggcgt cttttgatat ctgcgcttcc ttgaatcctg gtagcatttt
     5641 caccaatgca gataaacaaa cgataaacga aaaagcccgc atacgagaac aggaacagag
     5701 ccagaagcaa tctgcgaagt ctggtgagcc aggcatcatc cgcgtctgca acggtatcca
     5761 gagtcaggtt gagcttcgtg cgaatcgcat acgccgcaac tgccacaacg atcagcacaa
     5821 cagttgccag ataaccggac tgaaccctcg cccctctaga acgtgcagaa agaacaagaa
     5881 ccaccggatc ggaaacaatc acgatcgcag acaatgcgaa gaacacccaa ccggttggcc
     5941 catccagaaa ggaaaaggat gtaaacaaag tcaaagtggc gacggtctga atccagactc
     6001 ccgtccgcga atatacatct gaaagaatat caataaaatt tttcatccat tgcctcctgc
     6061 ttgcagacgc catcttcgcg attcaagaac gcgaacagtc aggaacagaa agaagattgt
     6121 aaggcttatg aaaaacacgc atgccttgag agaaatcacc cctcttgcga acggagcaaa
     6181 ctgactgaga ccgtgaaagt gcgtcacgat ttccttggca ggggagggaa gaactcctcc
//
LOCUS       DAOQJL010000183         6200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7146_length_6200_cov_5.096664, whole genome shotgun sequence.
ACCESSION   DAOQJL010000183 DAOQJL010000000
VERSION     DAOQJL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7146_length_6200_cov_5.096664"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLG78_09430"
     CDS             <1..916
                     /locus_tag="PLG78_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE23378.1"
                     /translation="LYIVNNLSIPASLWKSYSIYSGSTDANIQWWYGHNAVAFVLTTP
                     ILGMMYYYLPKHSRLPIFSHRISIIHFWSLIFIYIWAGPHHLLYQPIPDWAQTLGMVF
                     SVSLIAPSWGGMLNAFLTLTQAKDKIRTDATLKFILVAMTFYGMSTFEGPMMAIRSVN
                     MISHNTDWTIGHVHGGALGWVAGMMFVAMYYLVPRLWNTKLYSEKLAEIHFWMATVGV
                     LVYMVSMWVSGITEGLMWRAVDSTGALQYPDWIEIVRTLKPYRIARALGGTLFLSGFI
                     LMVYNLIQTIRRKGQGFVPVDLREAASK"
     gene            929..1597
                     /locus_tag="PLG78_09435"
     CDS             929..1597
                     /locus_tag="PLG78_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNE23379.1"
                     /translation="MSEEHKNLTFYEKLSEKIESKSLKFILFTTLAVLIGGLVEIPPF
                     FLMGSVQKIESVKPYNALELAGRDAYQQEGCFYCHTQVIRPFKWETDRWDRNREYGPE
                     PFSKAGEFVYEHPFLWGSKRTGPDLSHEASLNNNASWHRAHLMNPRETTPGSVMPAYP
                     WLFESKLDAAEVKSHMRALQTVGVPYTQTDMDAADELVKGKTQGDALIAYILKLGRDT
                     MVVK"
     gene            1606..1797
                     /locus_tag="PLG78_09440"
     CDS             1606..1797
                     /locus_tag="PLG78_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNE23380.1"
                     /translation="MEELIGIYKGLRMPLLGVVLILLAAYVYWPSRKKDLEQARFNML
                     DDDVDHDLSKKFTSKPDMQ"
     gene            1809..2345
                     /locus_tag="PLG78_09445"
     CDS             1809..2345
                     /locus_tag="PLG78_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE23381.1"
                     /translation="MSFNEYRDDDEIKESRGWLPTWWMVLFWGAIAFSVVYGVYMHGV
                     EGWSMAKQYREEIEAFEKAHPEKLVALNDDGTNPYRTDAAAIERGKKTFESICAACHK
                     VDMTGLVGPNLTDTTWLHGNTDKELYAIVMNGVPQEKLKQNPPKGPMPAHKNSLGSAK
                     VLEVLAYVASKNPSLKAK"
     gene            2364..3686
                     /gene="ccoG"
                     /locus_tag="PLG78_09450"
     CDS             2364..3686
                     /gene="ccoG"
                     /locus_tag="PLG78_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNE23382.1"
                     /translation="MVVARPVQGKHRTIKNRINVVLLILFLVLPFLRFGGNPFVLLDI
                     PGRKFHVFGLEIWPHEFYFLHLILIMSGLSLFFFTALLGRIWCGYGCPQTIFMDFYDW
                     GAKLLVGSEYGKRPLNTSQKIRQYLAFFILAIFFSFIFLAYFVPYEEIASDLMHARFF
                     AGPDTLAPAAWVVFMVMSVGFAVFNAGYFRENACKLVCPYGRFQTALLDTHSPIVSYD
                     VKRGEPRREGKQKLFEHGGDCIDCNMCNLVCPTGIDIREGLQVGCIACGLCVDACTNV
                     LSKFEKRTLIDYRTIEQVENPDAHRKYLRPRTVVYASLLLCFATAFVYLLVTRIPMYA
                     TVLRDRAISALYVPDIGYQNGYEVHVGNQSRKPIEVKIMVLDKRYEILAPQDTYKIGA
                     EGFEKIRFVVRSAQKEEFARTRPIEFKIVDVNEPTHERVVQTVFSYPI"
     gene            3763..4233
                     /locus_tag="PLG78_09455"
     CDS             3763..4233
                     /locus_tag="PLG78_09455"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNE23383.1"
                     /translation="MISLLLGATVASGIWFVKYAMSVQEPVMDDAYFAKGLNYQNRVA
                     SLDRAKAQGFAIATNLPANAPLSRSKQQLTFTVSGKTPIDNATLHIVIERPATTRERK
                     ILDLSFAQSAKISETERKFQTEVDFPGSGLWEISAEAAVNKDMAVYTRQTVTVQ"
     gene            4233..4373
                     /gene="ccoS"
                     /locus_tag="PLG78_09460"
                     /pseudo
     CDS             4233..4373
                     /gene="ccoS"
                     /locus_tag="PLG78_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
     gene            4478..5140
                     /locus_tag="PLG78_09465"
     CDS             4478..5140
                     /locus_tag="PLG78_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE23384.1"
                     /translation="MIEAVFAGAFVQGLTGSLHCPGMCGPFAHAFGRSGRISVSILYN
                     VMRTISYGLVGYLLGTSGGAINHLFESGIAAIVGGCLLVLLGLGLVFPRFSVHLRMPD
                     LFLKMIGNVMRSSTGAYAGPAFLGAISGFMPCGVLWPAYALALGTGVPWVGAVVMVFF
                     SFGTYPAMLAMGLSGGVWREKLSRPVYRVVFAILMIGLGIYTIVAHAFFSEICRSVAW
                     QS"
     gene            5128..>6200
                     /locus_tag="PLG78_09470"
     CDS             5128..>6200
                     /locus_tag="PLG78_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNE23385.1"
                     /translation="MAVITEKTKCYHCGNDATDTTFVLEVDSEKRAFCCSGCLTAYRI
                     IHEGGIESYYSHRDAYAPTVREDRDAGFYQAWEKRIPLRDGIRSGLFLIEGIHCASCV
                     WLNERVILAVGGVISASVQLSTSRAKISWNDSDTSLQKIAEALAKIGYRLVPIEETLE
                     SASRSQARGLLRRMTVAGFFAGNVMLLSVALYAGYFGSIDRYSKNFLQLASLMMTIPV
                     VLYSASVFFKNAYHALRNRTLSMDVLTATGISLAFSYSLYVYFSEVGEVFFDSICFVV
                     FIILTGRFIESRLRLKGLYYVENIRKSAAKTARVDREGWRTVLVDEVKKGDLVDVAAD
                     EMVPVDGVLRSENAEVEESMLTG"
BASE COUNT         1477 a         1454 c         1625 g         1644 t
ORIGIN      
        1 tctatacatc gtgaacaacc tttcgattcc tgccagtctg tggaaatcgt actcgattta
       61 ctcaggaagc acggacgcga acattcaatg gtggtacggt cacaatgctg tggcattcgt
      121 tttgaccact ccaattctcg ggatgatgta ttactatctt cccaagcact ctcgccttcc
      181 gattttcagt caccggattt caatcattca cttctggtca ttgattttca tctacatctg
      241 ggcgggtccg catcaccttc tctaccaacc aattccagac tgggcacaaa cgctcggcat
      301 ggtgttttca gtgagcttga ttgctccttc gtggggtggt atgctcaatg cgttcttgac
      361 gttgactcaa gccaaagaca agattcgaac agatgcaact ctcaagttca ttctggttgc
      421 gatgactttc tatggtatgt ctacgttcga aggaccaatg atggcgattc gatctgtgaa
      481 tatgatttcg cacaacactg actggacaat cggtcacgtg catggcggcg cactcggatg
      541 ggtggctggc atgatgtttg ttgcgatgta ctatctggtt ccacgtctct ggaatacaaa
      601 actctacagc gagaagctcg cagagatcca cttctggatg gcgacagtcg gcgtactggt
      661 ctacatggtg tccatgtggg tttctggaat tacagaaggc ctgatgtgga gagcagttga
      721 ttcaaccggt gcacttcagt atccggactg gattgaaatt gttcgcacgc tgaaaccata
      781 tcgcatcgcg cgcgcgctgg gtggaactct gttcctctct ggattcattc tgatggttta
      841 caacttgatt caaacgattc gtcgcaaggg tcagggcttt gtgccagtag atctcagaga
      901 agcagcgagc aagtgaggag tatgaactat gtcagaagaa cataagaatt taacatttta
      961 cgagaagctt tctgagaaga tcgaaagcaa aagtctcaaa ttcattctct tcacaaccct
     1021 ggccgttctg attggcggtc tggtggagat ccctcctttc ttcctgatgg gaagcgttca
     1081 gaagatcgaa agtgtgaagc cttacaatgc tctcgaactg gcaggccgcg acgcctatca
     1141 gcaagagggt tgcttctact gtcacacaca ggtcattcgc ccattcaaat gggagacaga
     1201 ccgctgggac agaaaccgcg agtacggacc ggagccattc tccaaagcag gggagttcgt
     1261 ttacgaacat ccattcctct ggggatccaa aagaaccggg cctgaccttt cgcacgaagc
     1321 aagcctgaac aacaatgcgt cctggcacag agctcacctg atgaatcctc gtgaaacaac
     1381 acctggttct gtgatgcctg cgtatccgtg gttgtttgaa tccaaactgg atgcggcaga
     1441 agtgaagtct cacatgcgcg ctttgcagac agtgggagtt ccttacaccc agactgatat
     1501 ggacgccgct gacgagcttg tcaaaggcaa gactcagggt gatgcgctca tcgcgtacat
     1561 tctgaaactt ggccgcgata cgatggttgt caaataggca aggcaatgga agagctcatt
     1621 ggaatttata agggtttgag gatgccgctt ctgggcgttg tcctcattct tcttgccgcc
     1681 tatgtatact ggccttcgcg caagaaagat cttgagcaag ccagattcaa catgctggac
     1741 gatgatgtgg atcacgatct ctcgaagaaa ttcacatcaa aaccggacat gcagtaaagg
     1801 agcaatgtat gtcgtttaac gaatacagag atgatgatga aatcaaggaa agccgcggct
     1861 ggcttcctac ctggtggatg gtgcttttct ggggtgccat tgcattctcc gttgtgtatg
     1921 gtgtgtacat gcacggagta gaaggttgga gcatggcaaa gcaataccgt gaagagattg
     1981 aagccttcga aaaagcgcat cctgaaaaac tggtcgcgct caatgatgat ggaaccaatc
     2041 cctatcgcac ggatgcagct gccatcgaac gcggaaagaa aacgttcgaa agtatctgcg
     2101 ctgcctgcca caaagtagac atgaccggtc tggttggtcc aaacctgacg gacacaacct
     2161 ggcttcatgg aaacacagac aaggaactgt acgcgatcgt aatgaacggc gtacctcaag
     2221 aaaaactgaa acagaatcca cccaaaggcc caatgcctgc tcataagaac agccttggat
     2281 ccgcaaaggt gcttgaggtg ctcgcatacg tcgcaagcaa gaatccttct ttgaaggcca
     2341 aatagggtaa gggagaaaat atcatggtag tagcacgtcc ggttcaggga aaacacagaa
     2401 cgattaagaa tcgcatcaat gtggttcttt tgattttgtt ccttgttctt ccgtttcttc
     2461 gctttggggg aaatccgttc gtgctgctgg acattcctgg cagaaagttc cacgtcttcg
     2521 gactggaaat ctggccccat gaattctatt tcttgcactt gattctaatt atgtccgggc
     2581 tgagtttgtt tttcttcaca gctctgctcg gacgtatctg gtgcggttac ggctgcccgc
     2641 agactatctt tatggatttt tacgattggg gcgcgaagct tctggtcggg tctgaatatg
     2701 gaaagcgtcc gctgaatact tcgcagaaga ttagacagta tctcgctttt ttcattctcg
     2761 caattttctt ctccttcatt ttcctcgcat actttgttcc atatgaagag atcgcatccg
     2821 atctcatgca tgccaggttc ttcgcaggcc cggatactct ggccccggcc gcctgggtag
     2881 tgtttatggt tatgtctgtt ggcttcgcgg tctttaacgc cggttacttt cgcgaaaacg
     2941 catgcaagct ggtctgtccc tacggacgct ttcaaactgc acttctggat actcactcac
     3001 cgatcgtcag ttatgacgtg aagcgcggcg aaccgcgccg cgaaggcaag cagaagctat
     3061 ttgaacacgg cggggattgc atagattgta atatgtgtaa cctggtttgt cctactggaa
     3121 tcgacatacg cgaaggcctt caggtgggtt gcattgcctg cggactttgc gtggatgcgt
     3181 gcacgaacgt tctgagtaaa ttcgaaaaac gcactctgat tgactatcgc acaattgagc
     3241 aggtggaaaa tccggatgcc catcgcaaat acctgagacc gcgcacggtc gtgtatgcgt
     3301 cgcttcttct gtgctttgca actgctttcg tttatctact cgttactcgc attcctatgt
     3361 atgccacagt ccttcgtgac cgcgcgattt ccgcgctcta tgtgcctgac attggctacc
     3421 agaacggata tgaggttcat gttggaaatc aatccaggaa gccaattgaa gtgaaaatca
     3481 tggtcctgga caagcgttac gaaatactgg cacctcagga cacttacaaa attggggcgg
     3541 aaggatttga aaagattcgc tttgttgtaa gatctgcaca gaaggaagaa ttcgcacgca
     3601 ctcgccccat tgagttcaag atcgtggacg taaacgaacc aacgcatgag agagtggtgc
     3661 aaactgtgtt cagttaccca atttagaagt gatagaaacg gaccaggaat caaagaggtc
     3721 aataaacctg ctatgtcaaa atcaatgaga atcgctttca tcatgatttc cctcctgctc
     3781 ggtgcaaccg ttgcgagcgg aatctggttc gtgaagtatg caatgagcgt tcaagaacct
     3841 gtgatggacg atgcgtattt tgccaaaggc ctcaactatc aaaatcgagt ggcttccctg
     3901 gacagggcaa aggcacaggg atttgcgatt gccacgaatt tgcctgccaa cgctccactg
     3961 tcacgttcaa agcagcagct gacttttact gtttcgggga aaacgcccat tgataatgcc
     4021 accctgcaca ttgtaattga aagaccggcc accacgcgcg agagaaagat ccttgatttg
     4081 agtttcgcgc agtctgcgaa aatttcagaa acggaacgta agttccagac agaagtggac
     4141 tttccaggat ccgggctctg ggaaatttca gcggaagctg cagtgaacaa agacatggcc
     4201 gtttatactc gacagactgt gacggtgcaa tgatgcctgg ccttcttttc atgcttccga
     4261 ttgcaatcgg cattgcgctg atttttctgg gattcttcct ctggagtgtg cgcaacgggg
     4321 actacgaaga tatggaaatg ccggcgtaca agatgacctt tgatcaggat gaaggcgaca
     4381 aatccattca gacttcgaac ggcgtttcgc cggtccacga agcaggttcc ctggcggttg
     4441 ctcaacctgc cgacgcgccc gatcccggga aatagaaatg attgaagccg ttttcgcggg
     4501 agcgtttgtt caggggctta caggttccct gcactgtccc gggatgtgcg gaccatttgc
     4561 gcacgctttt ggcagaagcg gccggatttc tgtttccata ctttataacg ttatgcgcac
     4621 tatctcgtac gggcttgtgg ggtatcttct aggaacttcc ggaggcgcca tcaatcattt
     4681 gtttgaatcg ggaattgccg cgatcgtggg tggttgcctt ctggtgctgc tcggtctcgg
     4741 gcttgtgttc ccgcgtttct ccgttcacct gcgcatgccg gacttatttc taaagatgat
     4801 tgggaacgtt atgcgctctt ctacgggggc ttatgccgga ccagcattcc ttggcgcgat
     4861 ttctggattc atgccgtgcg gtgtgctctg gcccgcttac gctctggcac ttggaacggg
     4921 cgtaccctgg gtgggtgctg ttgttatggt tttcttcagt tttgggactt atcccgctat
     4981 gcttgcaatg ggcctttccg gcggtgtctg gcgtgagaaa ctatccaggc ccgtctacag
     5041 agtagtgttt gccatcttga tgattggcct tggaatttac accattgtgg cgcatgcatt
     5101 tttttctgag atatgcagga gtgtagcgtg gcagtcataa ctgaaaaaac aaaatgttat
     5161 cattgtggaa acgatgcaac ggatactact ttcgtactgg aagtagattc agagaaacga
     5221 gcgttttgtt gcagtggatg tttgactgca tatagaatca tccatgaagg cgggattgaa
     5281 agttactaca gccaccgcga cgcctatgca ccaacggttc gcgaggatcg cgatgcagga
     5341 ttttaccagg catgggaaaa acgaattccg ttgcgagatg ggattcgtag cggcctgttt
     5401 ctgatcgaag ggattcattg cgcgtcgtgc gtctggttga atgaacgagt catccttgct
     5461 gttggtggag tgatttctgc gagtgtccag ctatctacca gcagagcgaa gattagctgg
     5521 aacgattcgg atacgtctct tcagaagatt gcggaagcac tggcgaagat tggctatcgc
     5581 ctcgttccca tagaagaaac actcgaaagc gcatctcgca gccaggcacg tggacttctt
     5641 cgtcgcatga ctgtggcagg cttctttgct ggaaatgtga tgctcctgag tgtggcgctc
     5701 tacgcaggct attttggttc gatcgacaga tattctaaga acttcctcca gcttgccagt
     5761 ctgatgatga ccattccggt cgttctctac tccgcttccg tcttcttcaa gaatgcttac
     5821 catgcgctgc gaaatcgcac tctgagtatg gacgttctga ctgccacagg aatcagcctt
     5881 gcgttttcgt acagtttgta tgtgtatttt tcagaagttg gggaagtgtt ctttgattcc
     5941 atttgttttg tcgtctttat tatcttaacc ggaaggttta ttgaatcccg tcttcgcctg
     6001 aaaggtttgt actacgtaga gaatattcgc aaatctgctg caaagaccgc acgcgtggat
     6061 cgcgaaggct ggcgcacggt ccttgtggat gaagtgaaga aaggggatct tgtggatgtg
     6121 gccgcggatg aaatggtccc ggtggacgga gtgctccgca gcgaaaacgc ggaagtggaa
     6181 gagtcgatgc tcacgggaga
//
LOCUS       DAOQJL010000184         6183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7176_length_6183_cov_4.769256, whole genome shotgun sequence.
ACCESSION   DAOQJL010000184 DAOQJL010000000
VERSION     DAOQJL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_6183_cov_4.769256"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1383
                     /locus_tag="PLG78_09475"
     CDS             271..1383
                     /locus_tag="PLG78_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23386.1"
                     /translation="MNKEIQKAFRDVVATSLNSDQVNALGQQMDRRFDIHELSGFGTK
                     IAIPGTAAAETLLHFLRTEERVVEFFERMLDQEGKFIYDSTVKLNYRDDFIRLLGKNK
                     WIFDVETKRFLRDPFFAEQLNFLKQLEMIDLRRSINLPAFSQQLQEDAVRLKLEDLNW
                     QVTIRTYRLTGEIATFLRDIISLLLMKQNLGSLSAEIYTCLNEMAINASKATYKVLFE
                     RHVAKPAGVDPKIEYNKFASMFREELEEHGDENLHKLAAAEDKYFDVIFRSTDANIAC
                     WTTNYTSISRPEKLRLLQRLNYTMFGENLTELMEDPHREGAGLGINLILTILGRLTPE
                     KHPLKPVFYQDRTKIGFVLKREALVNYVASRQTADT"
     gene            complement(1435..2625)
                     /locus_tag="PLG78_09480"
     CDS             complement(1435..2625)
                     /locus_tag="PLG78_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE23387.1"
                     /translation="MEISLKPESDLFDPTPAHRELREVVRKFSEGPLREQARHGDESE
                     KFNIDLFRRLGSELGILGLTVPEDQGGAALGPVAEVILHEELSKSDPAFTLSVLAHEI
                     LFVNNLSVNGNDAQRKKYLPGVISAQKIGGMCMTEPGAGTDVLSLTTVAVKKGDRYVL
                     NGSKAWITNGYEGDYFLVYAKMDNSSNKSITKFIVESAYPGFRVGKKEEKMGMRASST
                     AVLSFEDMEVPAENLVGEENRGLVSMMRNLEIERIALAAQSLGIAERCIEVMAKYAVS
                     DRSSFGKHLIEHGQIQRLVSESYADWSAARCLVYHTAGRLSPNVRNSTAAAAAKLVAT
                     QMAERVGRNAIQVLGGNGYSREYPVERLYRDAVLLSIGGGTNEAMQKNIANDLIKIFA
                     GPSH"
     gene            2682..3044
                     /locus_tag="PLG78_09485"
     CDS             2682..3044
                     /locus_tag="PLG78_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23388.1"
                     /translation="MQLSIPLLVFTVLCVGACAYLSVVIRQAYVHSLTSVEEKSAGII
                     HFYRARLRVLLSGAPDRMSILCGLHYLTVHLIPLVAGLSFYLKTDANFLVVILSIVWN
                     MQLFQRVFPGDSDAEDQT"
     gene            3113..3874
                     /locus_tag="PLG78_09490"
     CDS             3113..3874
                     /locus_tag="PLG78_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNE23389.1"
                     /translation="MKILVLIKQVPDTETSIKVSGAGIVDAGIKWIVSPFDEHALEEA
                     LRLKEKTGGTVIAASLGPDRVVEALRTAYALGADQAIHIKDDSYNVLDIGYAGSVLAK
                     FVEAEKPDLILTGHVAIDSQSSMVPSMIAQTLGCANINNAIELKVEGNKVVVKREIEG
                     GSAVMEASTPVVITAAKNLNEPRYPSLKGIMAAKKKQVDVKAADSFGSGFAKVEIVSL
                     ELPPPRPPGRIIDGDAPETKAKELVKALREEAKVI"
     gene            3893..4831
                     /locus_tag="PLG78_09495"
     CDS             3893..4831
                     /locus_tag="PLG78_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNE23390.1"
                     /translation="MAVLAVAELKGDSLKKISKELVSAGRTLGTPVVALLVNGNDAQA
                     KELLGAGADTVVKATLANYSPEGMANIVSAVAKEKGAKIVLAAHSVLGRDYAGRVAVQ
                     LGANVAADVVSIKGSLENVELRKPVYSGKAFANIRIKSPAVITVRPNSQEVKAHSGAE
                     AIETSSAGEGNVTGKITSTDMSGGSKIQLTDASIIVSGGRGIKGPENWPLLQALCDVT
                     GAALGASRAAVDAGWISHSAQVGQTGKTVSPNCYIACGISGAIQHLAGMGSSKYIVAI
                     NKDKDAPIFKVATYGIVDDLFKVVPAMTDEFKKVLG"
     gene            4824..5426
                     /locus_tag="PLG78_09500"
     CDS             4824..5426
                     /locus_tag="PLG78_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23391.1"
                     /translation="MAKSAYRLLFLTAAVAWQLAAVPLFAEPNPDVQAVVSRMRSLGG
                     FKASISISSGGGGALRGNLSYSNGKVHLALSDGRVIAATGRELVVYNPSSSVAGKQLM
                     VPGGGGLGWLLTGFKTKVSGNTALLESENPSSNIQEVRLKWGAEHTLEQLSIRHKSSG
                     DWTTITLSNLRKVDAFPVSLFSYDAPAGSRTVENPLNQSN"
     gene            5428..>6183
                     /locus_tag="PLG78_09505"
     CDS             5428..>6183
                     /locus_tag="PLG78_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23392.1"
                     /translation="MDPTLTRAAAHKAFGDLYRKARPPEVQEQVEKVIQSVSDVFARI
                     QRIEELDAAHARKQKGVETVERKRPSAGSGSGAAGAGAQRQAPKKPEPRRTGLLGAIL
                     GGGEIGVWGKDTETLTTGFLGRNLHISKKAEDIFSTLNEDVIVAVIKCFRVAARDGWT
                     QWDPPTYNVIVTAYQFFNEFITVAPMFKRVERQEVLAAESGKMQKMYAALIQYPNFRK
                     ILTDPFVKFVSESKELGGYTEALRNAMAFISSLD"
BASE COUNT         1669 a         1624 c         1475 g         1415 t
ORIGIN      
        1 ctggaatcat caaatccctg gcccccgccg gagcccggcc tgcactcttg cgcggaaaga
       61 aaacccgaga ggccagagtc tcatcgtcta acggattcat aaccgctctc ctttccgtgc
      121 aaccatgcac cagtagaact accaaaaggc caatccgagc acaaaggaac atgactcatt
      181 cacagggggc ccgggcaggc ctcaatccaa aaaatccggg cactagttct tgactggaat
      241 gcggcaatat ttcacaaatg ccaccacctg atgaacaagg aaattcagaa agcattccgc
      301 gatgttgttg caacgtctct gaactcagac caggtcaacg cgctgggcca gcagatggac
      361 cggagatttg atatccatga gctgtctgga tttggcacca agatcgccat acccgggacc
      421 gccgctgcgg aaacactttt gcatttcctg agaacagaag agagagtcgt tgaattcttc
      481 gagagaatgc tcgaccagga aggcaagttc atatatgact caacagtcaa attgaactat
      541 cgcgatgatt tcattcgcct gctcgggaag aacaaatgga tctttgatgt tgaaacaaaa
      601 agatttctca gagatccatt cttcgcagag cagctcaact tcctcaaaca acttgagatg
      661 atagatcttc gccgctctat caatctgcca gccttctctc agcaactgca ggaagatgcg
      721 gtgcgcctca agctggaaga tctgaactgg caggtaacca tccgtacata tcgcctgacg
      781 ggagaaatcg caacgttctt gcgcgatatc atttcacttt tactgatgaa gcaaaacctg
      841 gggtctcttt ccgcagagat ttacacctgc ctcaacgaaa tggccatcaa cgcatccaag
      901 gcaacttaca aagtgctctt tgaaagacac gtagcaaagc cggcaggtgt ggacccaaaa
      961 atcgaataca acaaatttgc atccatgttc cgcgaagaac tggaagaaca cggggatgaa
     1021 aaccttcaca agctcgcggc agcagaagac aaatacttcg acgtaatttt cagatccaca
     1081 gacgcgaaca ttgcctgctg gactaccaac tacacaagca tctcaagacc ggaaaaatta
     1141 cgccttcttc aaagactcaa ctacaccatg tttggggaaa acctgacgga gctcatggaa
     1201 gatcctcacc gcgaaggcgc gggactgggc atcaatttga ttttgacgat ccttggaaga
     1261 ctcactccgg aaaaacatcc gctcaaacct gtattctacc aggacagaac taagattggc
     1321 tttgtactca agcgtgaagc acttgtgaac tacgtcgcat ctcgccagac cgcggatact
     1381 tgagatccgc ttcatcttgc gtctaattac tccgtttaga gaatcacttt cccttcagtg
     1441 cgaaggacca gcgaagatct taataagatc atttgcaatg ttcttctgca tggcttcgtt
     1501 ggttccgcca ccaatgctca atagaaccgc gtcgcggtaa agacgttcca cgggatattc
     1561 acgactgtaa ccattcccgc ccagtacctg gatcgcattt cttccaactc tttccgccat
     1621 ctgtgttgcc acaagcttgg cggcggctgc cgcagtcgaa tttcgaacgt taggcgataa
     1681 tcttccagct gtatgataca caagacagcg agcggcagac cagtccgcat aactttcaga
     1741 aaccagtcgc tggatttgtc cgtgttcaat gagatgcttt ccaaaactgg atcgatcgct
     1801 cactgcgtac tttgccatca cttcaatgca gcgctccgcg attccaagtg attgtgcggc
     1861 cagagcgatt ctttcgatct ccagattgcg catcatggaa accaggccgc ggttttcctc
     1921 ccctacaagg ttttccgcgg ggacttccat atcttcaaaa gaaagaacag ctgtgctcga
     1981 cgcacgcatg cccatcttct cttctttttt cccgacgcga aatcctggat atgcagactc
     2041 aacgatgaac ttggtaatac tcttattgga agaattgtcc atcttcgcat aaaccaggaa
     2101 ataatcgcct tcgtatccgt ttgtgatcca ggctttggat ccattgagca cataacgatc
     2161 gccttttttc acggcaaccg tggtcaaact cagaacgtct gttccagcac caggttccgt
     2221 catgcacata ccacctatct tctgcgcaga aatgacccct ggtaaatact tctttctctg
     2281 cgcatcgttt ccatttacgg aaagattatt cacaaataga atttcgtggg caagaacact
     2341 caaagtaaac gccggatcgc ttttggaaag ctcttcgtgc agaatcactt ctgcgacagg
     2401 gcccagagcc gctcctcctt gatcttcagg aactgtgagg ccgagaattc caagctcgct
     2461 gcccagcctg cgaaacaagt ctatgttgaa tttttctgat tcgtctccat gacgcgcctg
     2521 ttcgcgcaat gggccttcgc tgaacttgcg aaccacctca cgaagctctc tatgggcggg
     2581 cgttggatca aaaagatcgg actctggctt cagcgatatt tccataggat gaaaagtttt
     2641 ccttgcactg tcccataaac aattttttct cttctcgatt catgcaactg tccattccac
     2701 ttctagtatt tacagtgctt tgtgtgggtg cttgcgccta cctgagcgtg gtcatccgtc
     2761 aggcttatgt ccactcactc acgagtgtgg aagagaagag cgccgggatc attcatttct
     2821 atcgcgccag actgcgcgtt ttgctttctg gagccccgga tcgcatgtcc atcctttgcg
     2881 gccttcatta cctcacggtt catctgattc ctctcgtcgc ggggctttct ttctatctaa
     2941 agacagatgc gaatttcctc gtcgtgattc tttctattgt atggaatatg cagctatttc
     3001 agcgcgtttt cccaggcgat tctgacgcgg aagaccaaac gtaaagtcat tgaccccggc
     3061 ccggaccacc aaaaagtcgt agaatccaag ttttatccat tggagatcct caatgaagat
     3121 actcgtactt attaaacagg ttcctgacac agaaaccagc atcaaggttt ctggcgcagg
     3181 aattgtagac gccggcatca aatggattgt atcgcctttt gatgaacatg cgctcgaaga
     3241 agcccttcgc ctgaaagaaa aaaccggcgg gactgtaatt gcggcatcac tcggcccgga
     3301 ccgcgtagtg gaagcgcttc gcactgcata tgcgctcggc gcagaccagg caattcacat
     3361 caaagacgat tcctacaacg ttctggacat tggctacgct ggttcagtgc tcgcgaagtt
     3421 cgtagaagca gaaaaaccag atctgatcct gactggccac gttgcaattg actctcagtc
     3481 ttccatggtt ccatccatga tcgcacagac tctcggatgc gcgaacatca acaacgcgat
     3541 cgagctaaaa gttgaaggaa acaaagtagt tgtaaaacgc gaaatcgaag gcggaagcgc
     3601 agtcatggaa gcttccactc cagtagtgat cacagcagca aaaaacctga acgaacctcg
     3661 ctatccttct ctgaaaggaa tcatggcagc caagaagaag caggtagatg tgaaagcagc
     3721 tgattcattc ggaagcgggt tcgcaaaagt agaaatcgtt tctctcgaac ttccacctcc
     3781 tcgtccacca ggccgcatca tcgacggtga cgcacctgaa acgaaggcga aagaactggt
     3841 taaggcgctt cgcgaagaag caaaagtaat ctgaggaaag aggtagaata caatggcagt
     3901 actcgcagta gcagaactca aaggcgattc gctgaagaaa atctcaaaag agcttgtttc
     3961 agctgggcgc accctcggaa ctccagtcgt tgcattgctt gtaaacggca atgacgcaca
     4021 ggcaaaagaa ctcctgggcg caggcgcaga tacagttgtt aaagccactc tggcaaatta
     4081 cagcccggaa ggaatggcaa acatcgtttc cgcggttgca aaagaaaaag gcgcaaagat
     4141 cgttctggca gctcattctg ttctaggacg tgactacgct gggcgcgttg cagtgcaact
     4201 cggagcgaac gttgcagcag atgtggtgag catcaaagga agtcttgaaa atgttgaact
     4261 gcgcaaacca gtctactctg gaaaagcgtt cgcaaatatt cgtatcaaga gcccggcagt
     4321 catcacagtc cgccccaact ctcaagaagt gaaagctcac tctggcgcag aagcaatcga
     4381 aacttcctca gccggagaag gcaatgtaac cggaaaaatc accagcacag acatgtcagg
     4441 tggatccaag attcagctca cagacgcgtc gatcatcgtt tcaggcggtc gtggaatcaa
     4501 aggcccggaa aactggccac ttctgcaggc tctgtgcgat gtaacaggtg cagcgcttgg
     4561 cgcatcccgc gctgcagtgg acgcgggatg gatttctcac agcgcgcaag ttggacaaac
     4621 tggtaagaca gtttctccaa actgctacat tgcatgcgga atctcaggcg ccatccagca
     4681 tctggctggt atgggatctt ccaaatacat cgtggccatc aacaaagaca aagatgctcc
     4741 cattttcaaa gtcgcaacgt acgggatcgt agacgatctg ttcaaagttg tgccggcaat
     4801 gacggatgaa ttcaagaagg ttcttggcta agtctgctta cagacttctg ttcctgacgg
     4861 cggcggttgc ctggcaactg gccgccgttc cgttatttgc agaaccaaac ccggacgttc
     4921 aggcagtcgt gtctcgcatg cgatccctgg gtggattcaa agcttctatc agcatatcct
     4981 ccggcggcgg gggagctctt cgcggcaacc tttcgtatag caatggtaag gtgcatctcg
     5041 cactctcaga cggacgagtg attgcagcca cgggacgcga actagttgtg tacaatccat
     5101 catcaagcgt cgcaggaaag cagctcatgg tcccgggcgg cggtggactt ggctggcttt
     5161 tgaccggatt caaaacaaaa gtcagtggga acacagcgtt actcgaatcg gaaaatcctt
     5221 cctccaacat tcaggaagtt cgtctcaaat ggggtgcgga acacacactc gaacaactga
     5281 gcattcggca taaaagctca ggcgactgga ccacaatcac tctcagcaac ctgcggaaag
     5341 tagacgcctt tcccgtaagt ttattttctt acgatgcccc cgcaggatct cgcacggtag
     5401 aaaaccctct caaccagagc aactgatatg gacccgaccc tcacgcgggc tgccgcgcac
     5461 aaggccttcg gcgatctata ccgcaaagca cgtcctccgg aagtgcaaga gcaagttgaa
     5521 aaagtaattc aatccgtcag cgatgtattc gcccgcatcc agagaattga ggaactggac
     5581 gcggcacatg cgcgaaaaca aaaaggcgta gaaacagtag aaagaaagcg accttcggcg
     5641 ggctccggta gcggagccgc aggtgccggt gcacagagac aggcaccaaa gaagccggag
     5701 ccgcgacgaa cggggttgtt aggtgctatc cttggcggag gagaaattgg tgtttgggga
     5761 aaagatacgg aaaccctgac caccggattc ctgggaagaa atcttcatat atccaaaaag
     5821 gcggaggaca tcttctccac tctcaacgaa gatgtaattg tagccgtaat caaatgcttt
     5881 cgcgtcgcag ctcgcgatgg ttggacccaa tgggatcctc ccacttacaa tgtgattgtg
     5941 actgcttatc aattcttcaa cgaattcatt acagtcgcac ccatgttcaa gcgagtggaa
     6001 agacaggaag ttctcgcggc agaatcgggc aaaatgcaga agatgtatgc tgctctgatt
     6061 caatatccaa acttcagaaa gatcctcacg gatccattcg ttaaatttgt ttcagaaagc
     6121 aaagaactgg gcggctatac cgaagccctt cgcaatgcga tggcattcat ttcatcgctt
     6181 gat
//
LOCUS       DAOQJL010000185         6174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7193_length_6174_cov_4.638993, whole genome shotgun sequence.
ACCESSION   DAOQJL010000185 DAOQJL010000000
VERSION     DAOQJL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7193_length_6174_cov_4.638993"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLG78_09510"
     CDS             complement(<1..342)
                     /locus_tag="PLG78_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNE23393.1"
                     /translation="MKKAIVFGLALSLTGIMTSANATVRKTANPVVFAHGLAGWDNIL
                     GAFYFGDNNGNFVGDPCDEFLEVLCNGGLNSSQKAYAASVQPFQSSEVRGTQLADKIQ
                     SYMTSVGASYVN"
     gene            615..3416
                     /locus_tag="PLG78_09515"
     CDS             615..3416
                     /locus_tag="PLG78_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE23394.1"
                     /translation="MRQSTMAGLILLAGLFVLPRAASAQIKPSVEILEDPTASLTSQT
                     ANISKNWKHFGSATPNLGYSRSTHWVRFDLEAPAAGEYIIEVAYPLIDLLECTILRAN
                     SESVETQGRLRSQADRLPLFHLSLAEKEKVRVIMRVQSDDSITFPVRIYTPQEHEHEQ
                     NTRQFLFGLYYGVIAVMILYNLFLYYFVRDQAYLYYVLTLVLLHGLFQLALNGFARMF
                     LGSELLWWKKGAIVFFHSAGVLAAILFCRVFLLIPRVAKKWDYIARAIIVWGALNMLI
                     SFFDLYRLVILSTVGLGILGIVFALASGIIAMRRGYRPARFYLLAWSALLFGGFIYVL
                     KIFGIIPSYWLTEYGVQIGSAVEATLLSIALGDRITTGRQDQVKARTEMLVHKRIALL
                     AQTELLNHLSQLDSLKSQMHSIEYEGNSLARLLENILETLRKILSFDKGFIVIADRLQ
                     KTYLRSVGSMQPDFYEKIPDDHYLHMLIKLPDALFDQVNRIVHLDRPVFENIPENSSQ
                     VTQVMKALEDTVAALRETGFSLCLPLAYEREIFGYVMLSARKSGEQYTNSELHLIDTF
                     RPSIAMAVRNAVLYEEVVLLRGRAEQKAAKLSDVIIDMKDTTRFDLKEKALVYISHPM
                     AEIYQTVRKYADKPQPVLISGETGTGKELIAQTIHEVGGLSREPFIPVNCAAIPASLW
                     ESEIFGHEKGAFTDAKSERAGKIEQAAGGTLFFDEIGEMPLELQAKLLRLIQERKYER
                     VGGKKSLEARCRFLFATNRDLQVMQKAGTFREDLYYRISVFQIVLPPLRERREDIPVL
                     VNFFIRKYAAEMQVPVSHIEKAAMESLTYYDWPGNIRELENCVIQTLVNSTSDTIRVE
                     DIPGHLSSRGQVVSKEALRTWDPDMDFRELDAMVSDYTRQVMIAAIQRCAGNKQEAAK
                     LLGVKRATFYYRLKELGIQ"
     gene            complement(3418..4755)
                     /locus_tag="PLG78_09520"
     CDS             complement(3418..4755)
                     /locus_tag="PLG78_09520"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNE23395.1"
                     /translation="MNRTEFGPDFVFGTATAAYQIEGAWQEDGKGESIWDRFSHTRGK
                     IENNQTGDVACDHYHRFESDLVILKSLGIQHYRFSISWSRIFPEGRGRVEQRGVDFYK
                     RLIAACAARGITPWVTLFHWDLPQALEDKGGWPARDTLDAFLEYVDFCTRTFSEIKNW
                     MILNEPMVFTGGGYLIGVHAPGKRSPSKFFAAAHHAALAQAEGGRITRANCPGAQVGS
                     TYSMTHAEAWSFAPRHQAARDRFDAVINRLFLDPACGRGYPWDTVPSLKKIQKHMKSG
                     DDAKLKCDFDFIGIQNYTRDVVRRSILMPYVWARPVSAKKRRVPETEMGWEVYPQGIY
                     ELLRKFGSYPEIKKIYVTENGAAFPDQVQDGRVHDEKRIAFLRAYMGEVIRAKRDGSP
                     VHGYFVWSFLDNFEWAFGYRPRFGLVHVDYETQVRIPKDSALFYRDLIAGKDV"
     gene            complement(4984..6111)
                     /locus_tag="PLG78_09525"
     CDS             complement(4984..6111)
                     /locus_tag="PLG78_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23396.1"
                     /translation="MSAREEIASLMLEGRFGEAYRKCDQALRGDSGGGQKSKVASDAD
                     FFRIAGAVSLLGLGHIRQAEEWTANVESTDPAFYYIEALIHLHAGRTHEALLCYTRLI
                     DEDPSDTFADRLIEKLRAHPDEIVSEVQETAIGRYLPIKHWFGEDGLIKRKTEEKPVS
                     PPLDRLRARFKSIRFTAKPVRWVMAGSIAFLFLVAAIFLYLKFGNRDHDLAERLPDPP
                     TQGTIIPLSELKKDEVRFTFDSRKEVIDQYELARRKVIDARVNEARVLLNEIDLSNAG
                     FELKERALLLKQMIPYIAPSRFSDNVEIQKVVEDPDLYQGVQVLWPGEIVNEPTGPVL
                     RTAGGKVRLNGSVPSSGTVKVSGFFQRIDRDPVVDVREIVR"
BASE COUNT         1560 a         1621 c         1514 g         1479 t
ORIGIN      
        1 gtttacataa gaagcgccta cgcttgtcat atagctttga atcttatccg ccagttgcgt
       61 tccgcggact tctgaactct gaaaaggctg cacggatgcc gcataagcct tctggctgga
      121 attcaacccg ccattgcaga gtacttccag aaactcatcg cagggatctc ccacgaagtt
      181 cccgttgttg tccccgaaat agaacgcccc cagaatattg tcccagccag ccagaccgtg
      241 cgcgaacaca accggattgg ctgtcttgcg tactgtggca ttcgccgatg tcatgatccc
      301 ggtcaaactc agagccagac cgaaaacaat ggctttcttc attctattcc tcctgtgcat
      361 tcgcacctgg cactacatgt gcatagatcg taccatgcaa ggcactcaca gatacgaatg
      421 gttgcgcggt gcaccagtcg caggagcgac taacgttcac gcgaaaatcg ctttcaccgt
      481 tggccaaagt gattagtgcg acgcaaaaaa taaaatcttc gtcttcgcac gcgcgcgaaa
      541 gaaaaagggt tgatttccaa tattcgggat tccaacattt cggaaattcc agatctttgg
      601 aacgcggggg aagcatgcgg cagagtacga tggcggggct gatcctgctt gcagggttat
      661 ttgtgcttcc acgcgctgca agcgctcaaa tcaagcccag cgttgaaatt ctagaagacc
      721 cgaccgcaag tctaacatcg caaactgcga acatttcaaa aaattggaaa cacttcggaa
      781 gcgcaacacc caacctgggg tattcgcgtt ccacccactg ggtccggttt gatctggaag
      841 caccggcggc cggagaatac atcattgaag ttgcctatcc gctgatagac ctcctcgagt
      901 gcaccattct gcgagccaat tcagagagcg tagaaaccca ggggcgcttg agatcgcaag
      961 cggatcgact cccattgttt catttaagtc tggcagaaaa agaaaaagtg cgagtgatca
     1021 tgcgcgtaca gagtgatgat tccatcacgt tcccggttcg catctatacg ccgcaggagc
     1081 acgagcacga acagaataca cgtcagtttc tttttggact gtactacggc gtcattgccg
     1141 tgatgattct atacaatcta ttcctgtatt acttcgtgcg cgatcaggca tatttatact
     1201 acgtactgac tctggtgctg ctgcatggcc ttttccagct cgctctcaac ggctttgcgc
     1261 gaatgttcct tggatctgag ctgctctggt ggaaaaaagg cgccatcgtc ttcttccatt
     1321 ctgctggagt gcttgcagcc atcctctttt gtcgcgtgtt tctactgatt ccacgagttg
     1381 ccaaaaagtg ggactacata gccagagcga taattgtgtg gggagccctc aacatgctca
     1441 tttcattctt tgacctgtat cgcctggtca tactatccac agtggggctt ggaatccttg
     1501 gaatcgtctt cgcgctggct tccggaatca tcgccatgcg aaggggatat agaccggcca
     1561 ggttctattt gcttgcctgg tctgctcttc tctttggcgg atttatctat gttctcaaaa
     1621 tttttggaat catcccgagc tactggctta cggaatacgg agtgcaaatt ggctctgcag
     1681 tagaagcaac tctcttatcc atcgctctgg gtgatcggat tacgactgga aggcaggacc
     1741 aggtcaaagc tcgcaccgaa atgctggtgc acaaaagaat tgcacttctg gcacaaacgg
     1801 aactcctcaa tcacctcagc cagctggata gtctgaagag ccagatgcat tccatcgaat
     1861 acgaaggtaa ctctctggct agactgctcg agaacatact cgaaactctt cgcaagattc
     1921 tgtcttttga taaaggattc attgtaatcg cggatcgact tcaaaaaacg tatctacgaa
     1981 gcgttggatc tatgcagccg gatttctacg agaagatacc ggacgatcac tatctccaca
     2041 tgctcatcaa attgccggat gccctgtttg accaggtgaa tcggatcgtg catctggatc
     2101 gtcctgtgtt tgaaaacata cctgaaaata gttcgcaggt cactcaggta atgaaagcgc
     2161 tggaagatac agtggctgca ctgcgcgaaa ctggcttcag tctgtgtctg cctcttgcct
     2221 acgaacgcga aatttttgga tacgttatgc tcagcgcgcg caagagcgga gaacagtaca
     2281 ccaattcaga acttcacctg attgatacat tccgcccttc catcgccatg gccgtgcgaa
     2341 acgcagtgct ctatgaagaa gtagttttgt tgcgcggtcg cgcagaacaa aaggcggcca
     2401 agctttcaga tgtgatcatc gacatgaaag acaccaccag atttgatctc aaagaaaaag
     2461 ccctcgtcta tattagccac ccgatggccg agatctacca gactgtgcga aagtatgcag
     2521 ataagccgca gcctgtactc atttcgggag aaaccggaac aggcaaagaa ctgattgcgc
     2581 agaccatcca cgaagtggga ggcctttcta gagagccgtt cattcctgtg aactgcgctg
     2641 cgatccccgc atctctctgg gaaagtgaaa tctttgggca tgaaaaaggt gccttcacag
     2701 acgcaaaatc agagagagcc gggaagatcg aacaggcagc agggggcact ctattctttg
     2761 acgaaatcgg cgaaatgcct ctcgaactcc aggcaaaact actgcgcttg attcaggaaa
     2821 gaaaatacga acgagtcggt ggaaagaaat ctctcgaagc acgatgcaga tttctcttcg
     2881 ccaccaaccg tgatctgcag gtaatgcaga aggcaggaac atttcgcgaa gatctctact
     2941 acagaatcag cgtgtttcag atagttctgc ctcctttgcg agaaagacga gaagacattc
     3001 cggtgctcgt gaatttcttt attcgcaagt atgcggcaga aatgcaggtg cctgtttcgc
     3061 acatagagaa agctgccatg gaatcgctca cctattacga ctggccagga aacatccgcg
     3121 aactggagaa ctgcgtcatc cagactctcg tcaatagcac ttcagataca atccgggtag
     3181 aggacattcc aggacacctc agttcgcgag gacaggtggt aagcaaagag gctcttcgca
     3241 cgtgggatcc tgatatggat ttccgcgaac tagacgcaat ggtcagcgac tacacaagac
     3301 aggtcatgat tgctgccatc cagagatgcg cgggaaacaa acaggaagcg gccaaactac
     3361 tcggtgtgaa gagagccacg ttctactaca gactcaaaga actgggaatt caatgaatca
     3421 gacgtctttt ccagcgatca aatcacggta gaaaagcgca gaatccttgg gaattcgaac
     3481 ctgggtttcg tagtccacgt gcaccagtcc gaatcttggg cgatagccaa atgcccattc
     3541 gaaattgtcc aggaagctcc atacaaaata accgtgaacg ggagatccgt ctcttttcgc
     3601 gcgaatcacc tctcccatgt atgcacgaag aaatgcgatt ctcttttcgt cgtggacgcg
     3661 cccatcttgc acttgatcgg gaaacgcggc tccgttttcc gtgacgtaga tcttcttgat
     3721 ttccgggtag gatccgaact ttcgcagaag ctcgtagatc ccctgaggat atacttccca
     3781 ccccatttca gtttcaggaa cgcgtctctt ctttgcagac accggcctgg cccagacgta
     3841 cggcatcagg atcgatcgcc ttacaacatc acgcgtatag ttctgaattc caatgaaatc
     3901 aaaatcgcat ttcagtttgg catcgtcgcc tgatttcatg tgcttctgga tcttcttcaa
     3961 agatgggact gtatcccacg gatatcctct cccacaggca ggatcgagaa acaaccgatt
     4021 gatcacagca tcaaaacgat cccgggccgc ctgatgacgc ggagcaaatg accaggcttc
     4081 cgcatgcgtc atggaataag tactgcctac ttgcgctcct ggacaattcg cgcgcgttat
     4141 gcgacctccc tctgcctgag cgagagctgc atggtgggcg gccgcaaaga acttcgaggg
     4201 cgatcttttg ccaggcgcgt gaacgccaat gagataaccg ccgccggtga agaccattgg
     4261 ctcgttcaaa atcatccagt tctttatttc agagaacgtt cgcgtacaga aatcaacata
     4321 ttccagaaaa gcgtctaacg tatcgcgagc gggccatcca cccttgtctt cgagtgcctg
     4381 tggtaaatcc cagtgaaaca aagtcaccca gggtgtgatc ccgcgcgcgg cgcaggcagc
     4441 aatcaatcgt ttgtagaaat caacgccgcg ctgctctacc ctaccgcgac cttccggaaa
     4501 aatgcgagac caggaaatag aaaagcgata gtgctgaatt cccagggatt ttaatataac
     4561 aagatcagac tcaaagcgat ggtagtgatc gcaggcaaca tccccggtct ggttgttttc
     4621 gattttgcca cgagtgtgac tgaaccgatc ccagatcgat tcaccttttc cgtcttcttg
     4681 ccacgctccc tcaatctggt aggctgccgt tgcagttcca aaaacgaaat cggggccaaa
     4741 ttcagttcga ttcactcatc aagcctggag gtcaaggttc ttgccacggt cttcaccggg
     4801 gacttcgatg gggttcatgc gatccaggta gcggtggatc tcctgcctgc tgaacaattg
     4861 ggccgctggc tccgctggag gcgccttgtg gaacggattg attggctgtg ctacagccga
     4921 tgtcacctga gcttccatct acttactaaa atagctcccg cgcgggaaga ctgcaagaaa
     4981 aattcagcga acgatttctc gaacatccac gactggatct cggtcgattc tctggaaaaa
     5041 tccgctgacc ttgactgttc ccgaagacgg aacggagcca ttcaggcgaa ccttgccacc
     5101 ggctgtgcga agaacgggtc ctgtgggctc atttacgatc tctcctggcc acagaacctg
     5161 gactccctgg tacaggtccg gatcctctac tactttctga atttcaacat tgtccgaaaa
     5221 acgcgaaggg gcgatgtaag ggatcatctg tttgagcaga agggcccgct ctttgagttc
     5281 aaagcctgca ttggataagt caatttcatt gagcaacact cgggcttcgt tcacgcgtgc
     5341 gtcgatgacc ttcctgcgcg caagctcata ctgatcgatg acctctttgc gcgaatcaaa
     5401 cgtaaagcga acttcatcct tcttcagttc tgaaagtgga atgatagtgc cctgggtggg
     5461 tggatcggga agtctctctg cgagatcatg atctctgttg ccaaacttga ggtacaaaaa
     5521 gatagcagcg accaggaaca gaaaagcaat ggaccctgcc atcacccatc tcaccggttt
     5581 tgctgtgaat cgaatcgatt taaaccgagc acgaagccga tcgagcggtg gcgaaacagg
     5641 tttctcttct gtttttctct tgatgagccc atcttcgcca aaccagtgtt tgatgggcag
     5701 ataacgtcca atggccgttt cctgaacttc gctcacaatt tcgtctggat gggcgcgaag
     5761 cttttcgatc aatctgtctg cgaatgtatc tgacgggtct tcgtctatga ggcgagtgta
     5821 gcagagcaaa gcttcgtggg ttctacctgc atgcaaatga atgagagcct ctatatagta
     5881 gaacgcagga tccgtagact cgacattggc ggtccactcc tctgcctggc ggatatgtcc
     5941 aaggcccagc aaggagacag cgcctgcaat gcgaaagaag tcagcatccg acgcaacttt
     6001 tgacttctgg cctccccccg aatcgcccct gagagcctga tcacacttgc gatacgcttc
     6061 gccgaatctg ccttcgagca tcaaggatgc gatttcttcg cgcgcactca tcagccaaac
     6121 aaccgttttt gtgcggacca cgcgttttca aattcttccg ctgtgattgg gatc
//
LOCUS       DAOQJL010000186         6154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7221_length_6154_cov_4.545499, whole genome shotgun sequence.
ACCESSION   DAOQJL010000186 DAOQJL010000000
VERSION     DAOQJL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_6154_cov_4.545499"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..2143)
                     /locus_tag="PLG78_09530"
     CDS             complement(35..2143)
                     /locus_tag="PLG78_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNE23397.1"
                     /translation="MLEEIRKNLRNADTVLGIGVILIFALIVVPLPGPLLDALIAVSL
                     LAGLLILLTALSSKGPADFTVFPTILLVTTIYRLAVNVSTTRMILSKGEASNSALVNA
                     FGTFVVGGSGMGSYVIGAVIFIIVMLVQIMVITKGATRVSEVAARFALDAMTGKQMAI
                     DTDLQNGHITEQEARQRRENVQKESNFYGAMDGASKFIQGDVRVGLIITGINIVGGLI
                     IGMTIRGEPFIDALARYTKFTIGDGLVNQIPALMISAATGVIVTRSVGEESLPTDIRK
                     QLFRNPQILYVVGAVLVMAGLLPGFPLLSMGTVGGFILFLGYRMDQSLKQAKIEDVRK
                     EKEKVEERKPESYTQQAKTDKLGVEIGYNLIPLVDPKSGGTLLDQISRLRGQFARDMG
                     LIVPPVRIRDNMSLQPNQYHVRLDGDVVGDFVLKPDHLIGLPTRVRDPLPGLEEFTEP
                     TYNMRAVWVPSDMKTDAENAGYDVVDPASVIATHLSSIIRKSSADIMGRQEIKEILDS
                     IRQDHPVIVSEVLDERKIPMGRIQEILKLLLREGVSIRNMVRILEVIAGQAEKQIYDP
                     YIVSEAVRMALRRQIVADYIQDGVLHCITVSPDIERRMRDAIHSDPTEGFILALKPDL
                     NIAIRDAFVQEFQASQQKGKMPVFMSSRVIRSPLFDYLIRFLPAGNFTVLAHEEVPQG
                     AGIKVEVSGQVALQKAEVAV"
     gene            complement(2143..3354)
                     /locus_tag="PLG78_09535"
     CDS             complement(2143..3354)
                     /locus_tag="PLG78_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNE23398.1"
                     /translation="MKMVHESDLDLSDPIEFEFSASLSFDFAGVDAVFSIPADALPHF
                     HLSRFAAEDEGRTEAPTERRLREEREKGRVARSQDLTNAVVLLGAVMGLYFTGGYMVN
                     QCMQLFHRFFSESAFAQTSFQAEDLKSFVGTLFFEVFKICMPLFGISMFMGIVGNVVQ
                     VGGLFTVRPLFMPERLTPDFTRIIPSRRNFYLLLKTLVLVGIIGFTAYMVIMDDFIPM
                     LKSAGMDTRQALVLFAHSAFKLLIVCAVLLLALAIPDYFYERFEYMENLKMDISEVKR
                     ERKEDFGDPLVRQRQRDRYFQLTRQRNMLKEVPKADVVITNPTHYAVALLYDPAVATG
                     PRVIAKGQDWLAFEIKRIARENDVPIEENPEVARAIYSAVEVGREIPENLYRAVSLIF
                     AKLDKFKAVRS"
     gene            complement(3351..4223)
                     /locus_tag="PLG78_09540"
     CDS             complement(3351..4223)
                     /locus_tag="PLG78_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNE23399.1"
                     /translation="MWTIRRTFSASSRDSKGIFLERFDHNTQYFLLIFARMVGMVFVA
                     PVFSADSIPYRTRMMIAFLLSAVLYPVSVNYLPPLPSGPVSFIGEATAQAALGIIIGF
                     LIQMIFAAFQIAGELFSVQSGLSFAEVLDPASQTTMPIIGTLKSSIGLLLFLSLNFPV
                     DGITAPAYLHMVRALAFSIKAAPTMMLDMTTLGGLLSTVDQTMGVLFVTALKIGIPAA
                     GILFIATVALGLIGRAAPQLNLMNMGVHINIILGLTLLLVLSPIYIPIMGGAFQSMYD
                     IIGTMLRDWPGKHS"
     gene            complement(4177..4440)
                     /gene="fliQ"
                     /locus_tag="PLG78_09545"
     CDS             complement(4177..4440)
                     /gene="fliQ"
                     /locus_tag="PLG78_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNE23400.1"
                     /translation="MLEADAIKLIQDALVVTLKVSAPMLILSLFVGLVVSILQTTTSI
                     QEQTLTFVPKLLAVFAALSLFASYIIQTLVDYTKDVFGLIQRF"
     gene            complement(4440..5216)
                     /gene="fliP"
                     /locus_tag="PLG78_09550"
     CDS             complement(4440..5216)
                     /gene="fliP"
                     /locus_tag="PLG78_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772588.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNE23401.1"
                     /translation="MSRAKLLTILALLFIAAGSLSAQVPGFQIPVIDGVRGARDGREA
                     SFSLLLIVLFTLLSLAPALVMMMTSFTKVVIVFDFVRRALSLQNMPPNQVLFGLALFV
                     TFFIMAPTVQEFNKSALQPYNRGELSTGKFMDEGMKPFRTFMIKQIGKDGAKEIGLFI
                     GLSGKDKNEIKTVDDIDSYILIPAFMLSEIKKAFIIGIVIFIPFIVIDLIVSSTLMSM
                     GMVMLPPAMISLPFKIILFILVDGWHLITYNLVISYNGGG"
     gene            complement(5310..>6154)
                     /locus_tag="PLG78_09555"
     CDS             complement(5310..>6154)
                     /locus_tag="PLG78_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNE23402.1"
                     /translation="VKEIDKSWMATIHGNPSDPKPDAAKTDDSSPNVTSTQDSARENA
                     DQSTAAKPGSAEKPDENPTRLRNPGSDPKSLFPDEDKPSFVGTLFRVLLMFVLMTGVF
                     YGVMRYLKSRSIGGSMGELATTLGSVALAPGKHLQVIDLAGKILVLGVSDAGVQLVTA
                     VDDARTADRIRIWHQSKPTLPKPENLIQKWLLGFGRADLKFWSRPEPPPPDFRTQLGQ
                     AVGPSFTAAEPAESVERVRPGASEILEQDLFGPEDELRRMLREQKNRLEKIREPGKKK
                     PSTG"
BASE COUNT         1601 a         1533 c         1508 g         1512 t
ORIGIN      
        1 agtgcttgcc agcactgcct atcggcactg cccgttagac ggcaacctcc gccttctgca
       61 gtgcaacctg tccgctcact tctactttga tgccggcgcc ctgtggcact tcttcatgcg
      121 caagcactgt gaaatttccg gccggcaaga aacggatcaa atagtcaaag agcggagaac
      181 gaatcacgcg gctggacatg aacactggca tttttccttt ttgctgcgac gcctggaact
      241 cctgcacgaa agcatcgcga atggcaatgt tgagatccgg cttgagagca agaatgaatc
      301 cttctgttgg atctgaatga attgcatccc tcattcttcg ttcaatgtca ggagaaacag
      361 taatgcaatg gagcacgccg tcctggatat agtctgcaac aatctgacgg cgaagagcca
      421 tacgcactgc ttctgagaca atgtagggat cgtagatttg cttttctgcc tgacccgcga
      481 tcacttcgag aatgcgcacc atgttcctga tcgaaacacc ttcgcgaaga agtagtttga
      541 gaatttcctg gatgcgcccc atagggattt ttctttcatc aagaacttcg ctgacgatga
      601 ctggatgatc ctgacgaatg ctatccagga tttccttgat ttcctggcga cccatgatgt
      661 cggcactcga tttgcgaatg atactcgaaa gatgcgttgc gatgacggat gcaggatcca
      721 ctacatcata gcctgcattt tcagcgtccg tcttcatgtc agaaggaacc cagactgcgc
      781 gcatattgta tgtgggctcc gtgaattctt cgagaccagg cagtggatct cgcacacgag
      841 ttggaaggcc aatgagatga tctggtttaa gtacaaaatc gccaaccaca tccccgtcca
      901 ggcgaacatg gtattgattc ggctgtagac tcatgttatc ccggatgcgc accggaggaa
      961 ctatcaggcc catatctcgc gcgaattgtc ctcgcagtcg cgatatttga tccagcaacg
     1021 tacctcctga ttttggatct acaagtggaa tcaagttgta gccaatttcg acgcccagtt
     1081 tgtccgtctt cgcctgctgc gtgtaagact ctggctttcg ctcttctact ttctcttttt
     1141 cctttcttac atcttctatt ttagcctgct tgagagattg atccattcta tatccaagga
     1201 atagaatgaa accacccacc gttcccatag acagaagagg gaatccagga agaagtccgg
     1261 ccatgacgag tactgcaccc accacataca gaatctgcgg gttgcgaaac aattgcttgc
     1321 gaatgtctgt aggtagactc tcttcaccca cgcttcgcgt aacgatcaca cccgtggcgg
     1381 cagaaatcat cagagctgga atctgattca cgagaccgtc accgattgta aactttgtat
     1441 accttgcgag tgcgtcgatg aacggctctc cgcgaatggt cataccaatg atcaaaccac
     1501 ccacgatgtt gatgcctgtg atgatcagac ctacccgtac gtccccctgg atgaacttgg
     1561 aagcaccatc catggctcca tagaagttcg attccttttg aacattctcc cgtctctgtc
     1621 gtgcttcctg ttctgtgatg tgaccattct gcaaatccgt atcaatggcc atttgcttgc
     1681 ccgtcatcgc gtcgagagca aaccttgctg ccacttcgga cacgcgcgtt gcaccttttg
     1741 taatgaccat gatctgcact aacatcacaa tgatgaaaat cacagcgcca atcacgtaac
     1801 tgcccatgcc gctgccgccc accacgaacg ttccaaacgc atttacaagt gcagagttag
     1861 acgcctctcc tttggaaaga atcatcctgg ttgtggacac gtttacagca aggcgataga
     1921 tcgtcgtaac aagtagaatg gttggaaaaa cagtaaagtc ggccggcccc ttggaagaaa
     1981 gggcggttag caaaatcaac agacctgcaa gcaaactaac tgcgatcaga gcatcgagaa
     2041 gcggacccgg aagtggaact acaatcaatg cgaaaatgag aatgacgccg atgccaagca
     2101 ccgtgtctgc gtttctcaaa ttcttgcgaa tttcttccaa catcagcttc ttaccgcctt
     2161 gaatttatcc agtttcgcaa agatcagact gacagcccgg tagaggtttt ccggtatttc
     2221 ccgtcccact tccacagcgc tgtatatggc gcgcgccact tcagggtttt cttctatagg
     2281 tacatcgttt tcgcgtgcga ttcttttgat ttcgaatgca agccagtcct gccctttcgc
     2341 aatcactcgt ggtccagtgg caactgcagg atcgtataga agagcaacag catagtgagt
     2401 cggatttgtg atgaccacat ccgcttttgg tacttctttt agcatgttgc gctgtcgcgt
     2461 gagctggaag taacgatctc tttgtctctg gcgcacaagt ggatcaccaa agtcttcctt
     2521 tctctctcgc ttcacttctg aaatgtccat tttcagattt tccatatact cgaagcgttc
     2581 gtaaaaatag tcaggaatcg ctagagcaag tagaagaact gcgcagacaa tcaagagttt
     2641 gaatgccgaa tgcgcgaaga gtacaagcgc ctgtcttgta tccatcccgg cagacttcag
     2701 cattggaatg aaatcgtcca tgatcaccat gtacgcggta aatccaatga tgcctaccag
     2761 caccagagtc ttgagcaaaa gataaaagtt ccttctgctt ggaatgatgc gcgtgaaatc
     2821 aggtgtgagt ctttccggca taaacagggg gcgaaccgtg aaaagaccgc ctacctggac
     2881 tacatttcca acaattccca tgaacataga aatgccgaat aacggcatgc agatcttaaa
     2941 tacttcaaag aacagcgttc caacaaatga cttcaagtct tctgcttgaa agctggtttg
     3001 tgcgaatgct gattcactga agaatctgtg gaacaactgc atgcactgat ttaccatgta
     3061 gccgccggta aaataaagac ccatcaccgc accgagcaat acaaccgcat tggtcaaatc
     3121 ctgcgaacgc gcgactcgac ctttctctct ttcttctcgc aggcgtcttt ctgttggagc
     3181 ttcggttcgg ccttcatctt ccgccgcgaa tcggcttaga tgaaagtggg gaagggcgtc
     3241 tgccggaatg gagaagaccg cgtccactcc tgcgaaatca aacgaaagag aagccgaaaa
     3301 ctcaaactca attggatcag agagatccag gtcagactca tgcaccattt tcatgaatgt
     3361 ttcccgggcc aatcgcgcag catcgtgcca atgatatcat acatggactg gaatgctccg
     3421 cccatgatcg gaatgtagat cggcgaaaga acgagaagca gtgtcaggcc caggatgata
     3481 ttgatgtgaa cacccatgtt catcaggttc aattgcggcg cggcgcgtcc gatcaatcca
     3541 agtgccacag tggcgatgaa tagaattcct gctgcgggga ttccgatctt gagcgccgtg
     3601 acaaagagaa ctcccatggt ttgatccacg gttgacagaa gtccaccgag agtggtcata
     3661 tcgagcatca tggtgggcgc agccttgatc gaaaacgcaa gtgcgcgaac catatggaga
     3721 taggcaggcg ctgtgatccc atccactgga aagttcaggc tgagaaaaag caaaaggccg
     3781 atgctgcttt tgagagtgcc aatgattggc atggtggtct gcgatgcagg atccaggact
     3841 tctgcgaaag acaatccaga ttgtacggaa aataattcac ctgcaatctg gaatgcggca
     3901 aagatcatct gaattagaaa tccgatgatg atgcccagtg ctgcctgtgc tgtcgcttct
     3961 ccgatgaatg agacgggccc ggaaggaagc ggaggcaaat agttgactga aacaggatac
     4021 agcactgcag aaagcaagaa agcaatcatc atgcgtgttc gatatggaat gctgtctgcg
     4081 ctgaatacgg gagcaacaaa caccattcca accattcttg cgaagatgag caggaagtat
     4141 tgagtgttgt gatcaaatcg ttccagaaaa atacccttag aatctctgga tgaggccgaa
     4201 aacgtccttc gtatagtcca caagggtctg aatgatgtag cttgcgaaaa gggagagcgc
     4261 ggcaaacaca gcaagcagct tgggaacaaa cgtaagagtt tgttcctgaa tggatgtggt
     4321 tgtctgaaga atggatacta cgagtccaac gaacagagaa agaatcagca tgggcgctga
     4381 tacctttagc gttacaacaa gtgcgtcttg aatgagttta attgcgtccg cttcgagcat
     4441 catccaccgc cgttatacga aattacaaga ttgtatgtaa tcagatgcca tccgtccacg
     4501 aggatgaaca aaatgatttt gaaaggcaga gaaatcatgg caggcgggag catcaccatg
     4561 cccatcgaca tgagggtgga tgaaacaatc agatcaatta ctatgaatgg aatgaaaatt
     4621 acgatgccaa tgatgaatgc cttcttgatc tcagagagca tgaaagccgg aatcagaatg
     4681 taggaatcaa tgtcgtctac ggttttgatt tcgtttttgt ctttgcctga caggccaatg
     4741 aacagtccga tctcttttgc gccgtctttt ccaatttgct tgatcatgaa tgtgcgaaag
     4801 ggtttcattc cttcgtccat gaacttaccg gtagatagtt ctccgcgatt gtatggttga
     4861 agcgcagact tgttgaattc ctgcacggtg ggcgccatga tgaagaacgt tacaaacaaa
     4921 gccaggccaa agagaacctg gttgggcggc atattctgga gagagagcgc gcggcgaaca
     4981 aaatcaaata caatgacaac cttcgtgaaa gaagtcatca tcattacaag agcgggtgcg
     5041 aggctgagaa gagtgaacag tacgatcagg agcagggaga aagatgcttc tcgcccatcc
     5101 ctcgctcccc ggactccatc gatcactggg atctggaatc cggggacctg ggcagacaaa
     5161 gaccctgctg caatgaaaag cagagccaga atcgtaagca gtttagctcg tgacataata
     5221 cggccccagg gcccaggttc ctccgctctg agatgagtac aagaatattt tagaagtaca
     5281 agtatttttt tggcaaaagc gggccttttt cagcccgtag atggcttctt tttgcccggt
     5341 tcacggatct tttccaggcg atttttctgt tcgcgtagca ttcttctgag ctcgtcttcc
     5401 ggcccgaaca agtcctgttc caggatctcc gatgcaccgg gtctcactct ctctactgat
     5461 tctgcaggtt cagccgcagt aaacgatggg cccactgcct ggccaagctg cgtgcgaaag
     5521 tcaggaggag gaggttcggg cctggaccag aatttcaaat cggcgcggcc aaaccccagg
     5581 agccatttct ggatcagatt ttcgggcttt ggtagagtag gcttgctttg atgccagatt
     5641 cgaatccgat cggctgtgcg tgcgtcgtcc actgcagtga ccagctgaac tcccgcatca
     5701 gacacgccca gtaccaggat ttttcctgca agatcaatga cctgtaaatg ctttccgggg
     5761 gccagtgcca ccgatccaag agtagtggcc agttctccca tgcttccgcc aatcgaacgc
     5821 gacttcaaat agcgcataac gccatagaaa acgcctgtca tgagtacgaa catcagtaga
     5881 acgcgaaaca gagttcccac aaaggacggc ttgtcttcat ccgggaataa tgactttgga
     5941 tcgctgcccg gattgcgaag tcgtgtcgga ttttcatcag gtttttctgc actgcctggt
     6001 tttgcggccg tggactggtc cgcgttttct ctcgcagagt cctgtgtgga tgtaacgtta
     6061 ggcgacgaat cgtctgtttt tgcagcgtct ggctttggat cagatgggtt gccgtggatc
     6121 gtcgccatcc aggacttatc aatttccttc acac
//
LOCUS       DAOQJL010000187         6152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7222_length_6152_cov_4.122027, whole genome shotgun sequence.
ACCESSION   DAOQJL010000187 DAOQJL010000000
VERSION     DAOQJL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7222_length_6152_cov_4.122027"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLG78_09560"
     CDS             complement(<1..409)
                     /locus_tag="PLG78_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23403.1"
                     /translation="MNCPHTGQALREILGGRAFQNEQGVIYVKGTRSLDYGADYFLTE
                     YEKQYGKTYLQDEPNLRRLAARRLEDLSRVAPPPASLFEIGAACGFFLDEARICGYDV
                     HGLEISEFASNHARSLGLDVQTASFPTATNKQYD"
     gene            complement(406..1881)
                     /locus_tag="PLG78_09565"
     CDS             complement(406..1881)
                     /locus_tag="PLG78_09565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE23404.1"
                     /translation="MTGLKNSGRILLAGSVLVLVVFTRLHNTIWPLTWPDEALFSSPA
                     NELASGRPFSTPVLSGLMYGMDRATLWNCPLHMVLLAGVYSVTGESRKAGRALSLCLS
                     VLSLGIFFLLARRISGEATAGAASLVLSLDPVFERAANTIRMDILTLLLILAALHLAL
                     RAREHKSYLLAFLGGLCGGLAGISHPFAAIVVPVLALALLPDARRLVASAAGVALGFL
                     PWAIYILRNQEIFAEQFIPQLQRKWTFAEMFLGGAGSETRGVFHVYFSQYGSYAGSWP
                     SMIAAGLLYVFACLVFLRACWLILQKQDDRLRVILCFFAVAGFALFSSEGWYVVYGDA
                     FLILGAAGVYRMADFRAARFLLLATALTMSGLLCYFTIRHKLLDTTRHAREMSSAMMK
                     AMSGCKAVYLRARPDPYFDLRNTQIRTYEFVPGKLKIKPERINDLKKTWDSIDCFLLE
                     RNPTWEPLLTEYLEQRKGEFRITSLEAPYPLYSSDLYRRDR"
     gene            complement(1856..2200)
                     /locus_tag="PLG78_09570"
     CDS             complement(1856..2200)
                     /locus_tag="PLG78_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE23405.1"
                     /translation="MPVVEVTSQLINDVLVVRLIGRLDIIGSEGLERKLVELLKNSTG
                     NILLNLKDVSYMSSSGISAVLGASRVASTMKRKLALCEISPSIQKLLEVVELGNIFPV
                     YETQGDGIEKLR"
     gene            complement(2428..3096)
                     /locus_tag="PLG78_09575"
     CDS             complement(2428..3096)
                     /locus_tag="PLG78_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE23406.1"
                     /translation="MGELKDFLASFLMPQRLARIRDVLSKRTNHITVVLEDIYDPHNA
                     SAVLRSCDAMGIQTVHIIQKRHTFQVKEGVSMGTGRWLDLRMHKETSECFDELRKNGY
                     SIASASPPLSGTVLLPDFQSPGKTAIVLGSEKDGLSPFARENSDCLITIPMFGFAESF
                     NLSVSGAILLYALRSRDGVPGLTTAESEALELEWIRKSLRNAEELEKAYHAKGDAEEG
                     SSRR"
     gene            3182..3574
                     /locus_tag="PLG78_09580"
     CDS             3182..3574
                     /locus_tag="PLG78_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNE23407.1"
                     /translation="MRVKISELKIGNRIRTNPGDLTALRDSMRRLGLLQPILIDSKYN
                     LIAGFRRLESAKMLGWESIEARLIETRDKKERLLIEADENTTRRDFSPEEMGRVDKLL
                     LRYSKGGIFWKFIAWLMDLFEWIFRRRS"
     gene            complement(3586..4653)
                     /gene="rlmN"
                     /locus_tag="PLG78_09585"
     CDS             complement(3586..4653)
                     /gene="rlmN"
                     /locus_tag="PLG78_09585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNE23408.1"
                     /translation="MSEETPILKGALLEELTEFFKSIGEPGYRGKQAFIRINRFQART
                     LSEFTEFPLALREKLEAMGAFPDLPIIQTSKDADGTEKAVFAAGTDRRDEETKKIEAV
                     WIVSEGRRTACISSQVGCTLNCAFCATGTMKFRGNLKAWQIVDQVYGLMRHRGSSMAA
                     DDPERLTNVVFMGMGEPFYNYDQALRAAHLLHNPHGLNLGARHITISTAGVVPGIERF
                     TSEGQPFNLAISLNHPDPEGRRDIMDITDRFSLPDLLAAVRAYTKASGKPVMFEFVMI
                     PDKNMGKENVEKLVAIAKSVNCKINLIPLNTNLHNWRRPTDEEMVEFQNALRQEGILA
                     FNRGSPGRRVGGACGMLALTG"
     gene            complement(4658..5689)
                     /gene="purM"
                     /locus_tag="PLG78_09590"
     CDS             complement(4658..5689)
                     /gene="purM"
                     /locus_tag="PLG78_09590"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNE23409.1"
                     /translation="MDYKTAGVDTHAGQQFVKLIGPAVASTKKPHVLGGLGGFAAMSD
                     LSFLKEYKRPVLLSTTDGVGTKLHLAKLFNRHETVGIDLVAMCANDLLVTGARALIFL
                     DYVACGKLNPEQMAIVVQGVAEGCKQAGCSLVGGETAEHPGLLDNDEYDLAGFCAGVA
                     EHDLLITGKEIREGDVILALPSSGIHSNGVSLIRKIFLKEDRLPDSASDRDFLLNDIL
                     LKPTRIYEPFLRGAIDNHPGAITGMVHITGGGFYENVPRIIPDGLCADIDTALIPVPA
                     LFDHIMKKGSVARKEMYSVYNMGAGMIMAVRPDQVESVVASIQQAGLSAHKAGIVIRN
                     TEKVLLRGI"
     gene            complement(5761..>6152)
                     /locus_tag="PLG78_09595"
     CDS             complement(5761..>6152)
                     /locus_tag="PLG78_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23410.1"
                     /translation="IATNDTGVIQYRAKDKIGNETAIQTYSFRIDSVAPQLPTIFQGL
                     QEPYRIPREGLSVADFPKNAIVEYKIDDSDFRKLEPGDRIVIDKEGEHTVTIRVTDEV
                     GNVTEKTYKVLVDNTPPKSELRAEIAP"
BASE COUNT         1429 a         1600 c         1585 g         1538 t
ORIGIN      
        1 catcgtattg tttgtttgtc gctgtcggaa aggaagctgt ttgcacatcg agccccagac
       61 tccgtgcatg gttggaggca aactcagaga tttccagacc atgtacatcg tatccgcata
      121 tgcgtgcttc gtctaaaaag aagccacagg cagctccgat ttcaaacaaa gatgcgggag
      181 gaggcgctac gcgggaaaga tcttccagtc ttcgcgcagc aagtcttcgc aggttgggtt
      241 catcctgtaa ataggtcttg ccatattgct tttcgtattc cgttagaaaa tagtctgcgc
      301 cgtagtccag actgcgggtg cccttaacgt agatgacccc ttgctcattc tggaaggcac
      361 gtccgcctag aatttctctg agagcctggc cggtgtgcgg acagttcacc gatctctccg
      421 atacaggtca gacgagtaga gcggataggg cgcttccagg gacgttatgc ggaattctcc
      481 ctttctttgt tccagatact ccgtaagcaa tggttcccac gtgggatttc tttcgagtaa
      541 aaagcaatca atcgagtccc aggtcttttt cagatcgttt atacgttccg gtttgatttt
      601 gagtttaccc ggtacgaatt cgtaggttct gatttgtgta ttgcgcagat caaaatatgg
      661 atcaggtctc gcgcgaagat acactgcttt gcagccgctc attgctttca tcatggcgga
      721 gctcatctcg cgcgcgtgac gcgtcgtatc cagcagtttg tggcgaatgg taaagtagca
      781 gagaaggccg gacatcgtaa gggccgttgc gaggagcagg aatcttgccg cccgaaagtc
      841 cgccatgcga tagactcctg ctgcgcctag aatcagaaac gcatcaccgt aaactacata
      901 ccacccttcg gaagagaaga gcgcgaaacc agcaactgca aagaagcaaa ggatcacacg
      961 tagcctgtcg tcctgctttt gcagaatcaa ccagcatgcg cgcagaaaaa caaggcaggc
     1021 gaatacatac agaagacccg ccgctatcat cgaaggccag gagccggcat aggagccgta
     1081 ctgactgaag tacacgtgaa acactccgcg tgtttcgctt cccgctccac ccagaaacat
     1141 ttcagcaaac gtccacttgc gttgcagctg gggaatgaat tgttctgcaa agatctcctg
     1201 attgcgcagg atgtagatgg cccagggaag aaatccaagc gccactccgg ccgcactcgc
     1261 aaccaggcgg cgcgcgtcag gtagaagggc cagcgcgagc acaggcacta cgatggccgc
     1321 gaatggatgt gagatgccgg ccaggccgcc gcagagtccg ccaagaaatg ccagaagata
     1381 actcttgtgt tcccttgcgc gaagagcgag atgcaatgcg gcaagaatca acagaagagt
     1441 gagtatatcc attctgattg tgtttgccgc tctttcgaat actggatcca gactcaagac
     1501 cagcgatgca gcaccggcag tcgcttcccc ggaaatgcga cgtgcgagaa ggaagaagat
     1561 tccaagagat agtacgctca ggcaaagtga aagcgcgcgt cctgcttttc tgctttctcc
     1621 ggtcacagaa taaacacctg caaggagaac catatgcaga gggcaattcc agagagtcgc
     1681 gcggtccatg ccgtacatca gaccggaaag gactggagta gaaaatggtc tgccgcttgc
     1741 gagttcattg gcaggtgaag agaaaagagc ttcgtcgggc caggtaagag gccagatagt
     1801 attgtggagt cgtgtgaaga cgaccaggac caggacagaa ccggcgagga gaatcctacc
     1861 ggagtttttc aatcccgtca ccttgcgttt cgtagacggg aaagatattg cccagttcta
     1921 ctacttcgag cagcttctgg atcgacggcg agatttcgca gagcgccagt ttgcgtttca
     1981 tggtggatgc aactcgtgac gcgcccagca cggcagaaat gccggacgag gacatgtagc
     2041 ttacgtcttt gaggttgagc agaatgttgc cggttgaatt cttcagcagc tctacgagct
     2101 tcctttccag accttccgaa ccaatgatgt ccaggcggcc gatgaggcgc actacgagta
     2161 cgtcgtttat gagctgcgag gttacttcta caacgggcac gcggtcaaat tacccgtgca
     2221 agcacccctg gccagccttt ttacgggtcg ggctttgtgg gttgatcgag ccagggggcc
     2281 cagactcgct tccacgaatc tgtccacgtg taccatctgt cccgagtctt gcggttttct
     2341 tccagctcgc ggtctaccgt ttcacacagt tccgccagcc tctcgcgtcg attctggatc
     2401 cgatcccaga gctcgtccat aggctaatta tcggcggctt gagccttctt ccgcatcccc
     2461 ttttgcatgg tatgccttct ccagctcttc tgcattccgt agagattttc ggatccattc
     2521 gagctccagg gcttcacttt ccgcagtggt aagcccaggc acaccgtcgc gtgagcgtag
     2581 agcgtacaat aaaatggcgc ccgaaacaga caggttgaaa ctctccgcga atccgaacat
     2641 gggaatcgta atcagacagt ccgagttctc gcgcgcgaac ggggaaagcc catctttttc
     2701 agatccaagt acgatggccg ttttgccggg cgactgaaaa tcggggagca gcactgtgcc
     2761 cgaaagagga ggagaagcgc ttgcgatgga gtacccattc ttgcgcaatt cgtcaaagca
     2821 ctctgaagtt tctttgtgca ttcgcagatc caaccaccgg cctgtcccca tcgacactcc
     2881 ttccttcacc tggaaagtat gcctcttttg aatgatgtgg accgtttgaa ttcccatggc
     2941 gtcgcagctt cgtaacaccg cgcttgcatt gtgcggatcg tatatatctt cgagcactac
     3001 tgtgatatga ttggtgcgtt ttgatagaac gtcccggatg cgcgccagtc gctgcggcat
     3061 taagaaggat gccagaaagt ctttcaactc tcccataatt tgtcacatat agcatccgct
     3121 tgcggcatga aaaggcgata aaacggcttg cgctcggaag gaactccgcc aggatcacac
     3181 catgcgggtg aaaatctctg aactgaaaat cggtaaccgg attcgtacga atcctggtga
     3241 tctgacagcg ctcagagatt ctatgcgtag actcgggctg ctgcagccaa ttctgatcga
     3301 ctcaaagtac aatttgattg caggcttcag aagattggaa agcgcgaaga tgcttggctg
     3361 ggaatctatt gaagcgagac tcattgaaac ccgggataag aaagaacgcc tgctcattga
     3421 agcagatgag aataccactc gccgcgactt ttcgccggaa gagatgggcc gcgtagacaa
     3481 gctccttctt cgatacagca aaggcggcat cttctggaaa ttcattgcat ggctgatgga
     3541 tttgttcgag tggatctttc gccgtcgcag ctgattgggg cgcgttcaac cagtcaacgc
     3601 cagcataccg cacgcaccac ccacacgcct tccgggggag ccgcgattga atgcgagaat
     3661 cccttcctgg cgtagcgcgt tctgaaattc taccatctct tcatccgtag gtcttcgcca
     3721 gttgtggaga tttgtgttga gtggaatcaa attgattttg caattcacag atttcgcgat
     3781 cgccacgagt ttttctacat tctcttttcc catgttcttg tctggaatca tcacgaattc
     3841 aaacatcact ggcttaccgc ttgccttcgt gtatgccctt acagctgcca atagatcggg
     3901 aagagaaaat cgatctgtaa tgtccataat atcccgacgg ccttctggat ccgggtggtt
     3961 gagcgaaatg gcaagattga atggctggcc ttcgctcgtg aatctttcaa tccctggaac
     4021 tactcctgca gtagaaattg taatgtgccg cgcacccagg ttcaggccgt gaggattgtg
     4081 aagtagatgc gccgcgcgca atgcctgatc ataattgtag aagggctctc ccattcccat
     4141 gaacactaca ttggtcaatc tctctggatc gtctgctgcc atactcgagc cgcggtggcg
     4201 cattagaccg tacacctgat ctacaatttg ccaggctttg aggttgccgc gaaatttcat
     4261 ggtgccagta gcacagaatg cgcagttgag tgtgcatcca acctgagacg aaatgcacgc
     4321 tgtgcgtctt ccctcggaga cgatccacac agcctctatt ttctttgtct cttcgtctct
     4381 gcgatcggtt ccggcggcaa agactgcttt ttcagttccg tccgcgtcct tgctcgtctg
     4441 gatgatgggc aaatcgggga acgcacccat ggcctctaac ttttcgcgga gtgccagcgg
     4501 gaactctgta aactcagata gagttcgcgc ctggaatcga ttgatccgaa tgaatgcctg
     4561 tttgccccgg tagcccggct ctcctatgct tttgaagaat tcagtcagct cttcgagcag
     4621 agcgcctttc aggataggag tttcttcgct catgcttcta gattcctctc agaagcactt
     4681 tctccgtgtt cctgatgacg atccctgctt tgtgcgcgga gagtcctgcc tgctggatgc
     4741 ttgcaacaac ggactctacc tgatccggac gcactgccat gatcatccct gcacccatat
     4801 tatatacgga atacatttcc tttctggcta cactcccttt cttcatgatg tgatcaaaaa
     4861 gagcgggcac aggaatcaga gccgtgtcta tgtccgcaca cagcccatcg gggatgatgc
     4921 gcgggacgtt ctcgtagaaa ccgcctccag tgatgtgcac cattccagtg atggctcctg
     4981 ggtgattgtc aatggccccg cgcaggaatg gttcgtagat tctagttggc ttcaaaagaa
     5041 tgtcatttag aagaaagtct ctgtctgaag cagaatctgg aaggcgatcc tctttcagga
     5101 agatcttgcg aatcagagag actccgttgg aatgaatgcc ggatgacggt agtgcgagga
     5161 ttacatctcc ttctcgaatt tcttttccgg taatgagaag atcatgttcg gccactccag
     5221 cacagaatcc tgcgaggtca tattcatcat tgtccaggag tccaggatgt tctgctgttt
     5281 ctcctcctac aagagagcaa ccggcctgct tacagccttc ggcgactccc tgaactacga
     5341 tcgccatttg ctccgggttc agcttcccgc acgctacata gtccagaaag atcaatgccc
     5401 tggctccggt gacaagcaag tcgttggcgc acatggccac caggtcaatc cccacagtct
     5461 catgtcgatt gaacagttta gcaaggtgaa gctttgttcc tacaccgtcc gttgtggaaa
     5521 ggagtacggg tcttttgtat tcttttagaa aactcagatc gctcatggcc gcgaatccgc
     5581 cgagtcctcc cagaacgtga ggtttcttcg tactcgcaac tgcaggacca atgagtttta
     5641 caaattgttg tcctgcatga gtgtcaacgc cggctgtttt ataatccata agcatccatt
     5701 ttctttagca aaaaaaaagg ccggtttccc ggccttttct cttttagaga tcagagttga
     5761 tcaaggagca atctctgctc tgagttccga tttaggcgga gtattgtcta cgagcacttt
     5821 gtaagtcttc tctgtcacgt ttccaacttc gtcggttacg cgaatggtta cagtgtgttc
     5881 gccttctttg tcgattacga tgcgatctcc tggttccagt tttctgaaat cagaatcatc
     5941 aatcttgtat tcaacaatag cattctttgg gaaatccgca acgctcagac cttcgcgtgg
     6001 gatgcggtaa ggttcctgca gtccctggaa gatggttggg agttgtggtg caacgctgtc
     6061 gatgcggaat gagtaagtct ggatagcggt ttcattcccg atcttgtcct tcgcgcgata
     6121 ctgaatcact ccagtgtcgt ttgttgcgat gg
//
LOCUS       DAOQJL010000188         6119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7284_length_6119_cov_4.480706, whole genome shotgun sequence.
ACCESSION   DAOQJL010000188 DAOQJL010000000
VERSION     DAOQJL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7284_length_6119_cov_4.480706"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLG78_09600"
     CDS             complement(<1..240)
                     /locus_tag="PLG78_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23411.1"
                     /translation="MNAILCVVGSIPFFWAAFAPGSFEFVRDSGRIIQLSIVVLLVIL
                     AWIRHRDPESTLPAFLESKHWHLLLIVASSLHLLVF"
     gene            complement(237..1622)
                     /locus_tag="PLG78_09605"
     CDS             complement(237..1622)
                     /locus_tag="PLG78_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23412.1"
                     /translation="MLSHSNRSASGMIWAFPAGQYGGLWALARTDGYSPVSHIWLETP
                     CDVSDHYPDRFTGSPGESFYLWNTAIERSIPVLVRLHRASICLFEVIPEKPMRAGDTY
                     VLVVRDVLNRSGNPVVLSHLFDKGADYETLLSERLVPMPSNFRKFGVQTILHITIRSY
                     ENMIGQFLHLRNSILAWTDPDDVPVSITEILQNPGQLIVHGTAQLPTICERDLFGHCR
                     ITPSDTGRIEYNRSTITRDFFVRIPETTARRGLFIPAMVYQEPETLIQLNGVARLLDD
                     TGSAAFGFRDQKNFLSLNRKPVYDLPSPLPGYILDQANHDRLTAMPDREAVRVLHGIL
                     LSRLMQSRIPALISGRVKRPLRIDKIEGLALEADRDAFALSVNPFLSGDVLLFADDEP
                     RGLPFGPGGTIAEKEYVRLYWDGMHFPNYAPLIEGRGTMPDTPRRKTLVVRSILIEEE
                     RKKVVRFLNGP"
     gene            1852..2451
                     /locus_tag="PLG78_09610"
     CDS             1852..2451
                     /locus_tag="PLG78_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL21"
                     /protein_id="HNE23413.1"
                     /translation="MKKWYLIGAIALSSMVLYCGGSGNDARRDATSTGPDGWIFEGWA
                     CAPDAAKALLKQSPAQYCQDDKPKDFLYMKFSAAASQNAIDKGMVAMKQSTCRKAAKD
                     QISSDGLAKILGEYLEQASGVKDGQSTGQVIIAQNQGIIRGVSIYNCCSLDQQTGVCA
                     TAGAAETWDQCQCVGVMKYPGGQKGFEAAAEKASSSANP"
     gene            2597..5005
                     /locus_tag="PLG78_09615"
     CDS             2597..5005
                     /locus_tag="PLG78_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE23414.1"
                     /translation="MARIPLLCILFLGSCARMNEATLDLPSDWRIVSGFRDAANPLTD
                     GMKVRLPVRPNEIFPDFKGSTAHYTLETTFALTPDEITRLPDPAFFFPMLGEGYNLYL
                     NGNSIVENLRLHPDGELSAVTTGRGLIVPFPSHLLRAGENRLSIHLAGFTRRTALSSN
                     SVDFGLPVKGGYRLASHTTLMRENSEMALYAFCGVYAVFAFYSLLYFVRRPKHIYTLY
                     FALFTCTYSVYLICLSNRAFASVADVRTVICIAYAAQPAALGFFVAFLNAYTGAGFRK
                     TTRLMIAFDSILAIAALSLPYPYLQSWLIVFYMVMAPQLILVLYIIGAVVKSRAPGSA
                     FAAASISLGLGFVVWDILDTVFFRTSIRLSRYAHFPVILAMMLMLVNRSLASMEEVEK
                     LNLELSFATKDLDQRVQERTRQLEAAMSSAKRADRAKTSFLANMSHEIRTPLNVLIGM
                     GDWLHEREEDSEKRRVLASMLTAGNGLLTLIQELLDVARIESGTIRLDSVPFDAEAIA
                     QDVVHFFEGRAKEKSLSLEYQSPAKRIGPLRGDPARIRQVLVNLLGNSLKFTETGSVR
                     LTLTLLSETSDTSKIRFLVKDTGPGIAPEDTRTIFERFAQGVSSMNRKHAGTGLGLSI
                     SQDLLKLMGSKIDLTTTVGHGSEFAFTLDLPKSADVIAEIVHSPELPDERPLRILVAE
                     DDEVNRTLLSMYFAQSAHTLEFAHNGLEAVRAFESHPFDVIFMDLMMPEMDGIEATIR
                     IRKIETELLSSGVRTQKTPILALSASTYAEDVALSKQAGCDEHLAKPVKRATLFAALA
                     RVTS"
     gene            complement(5061..>6119)
                     /locus_tag="PLG78_09620"
     CDS             complement(5061..>6119)
                     /locus_tag="PLG78_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HNE23415.1"
                     /translation="ETQVENTDQSRFEILVVEDDEIQSRLLREFLEILRYSVREAPTC
                     ADALRLIHERRPDLTLLDVHLPDGSGLDLVQQIHLIDKDASVVLMSADRTAEMLVESL
                     AKGAEEFLEKPLDLGELAIRIQNLLQTVAFRKKSEELQAELAREKEILTRYFSAETVT
                     SILTGEMEADASGQTGVATILIFDLRNSTGLAEIMGAIPFAELLNSLMIALMDLIFEK
                     NGSIAKLTGDGLVATFLPGQACAAISCAQSVAQYFQSIAQESSSEPARKLGFGIGIAT
                     GKVFQGNVGTFRRLEWTIVGDAVARAARLESLTKKLGCSILIDRPTMRGCGSQETSAR
                     EKRVLIKGRKATLYTLAD"
BASE COUNT         1449 a         1536 c         1566 g         1568 t
ORIGIN      
        1 gaacacaaga agatgaaggg atgaagctac aatgaggagc agatgccagt gtttgctttc
       61 caggaatgcg ggcagagtgg attcaggatc gcgatgacga atccaggcaa gaattacgag
      121 caaaactaca atagaaagct ggatgattct ccccgaatcg cgcacaaatt caaacgatcc
      181 tggtgcgaag gcggcccaga agaatggaat tgagccgacg acgcacagaa ttgcgttcac
      241 ggtccattta agaatcgcac tactttcttg cgttcttcct caatcaagat gcttcgcaca
      301 accagagttt tgcggcgagg agtatccggc atggttcccc tgccttctat gagcggggcg
      361 tagtttggga aatgcatgcc atcccagtag agtcgcacat attctttttc tgcaatcgtt
      421 ccgcccggtc cgaatggcaa tccgcgcggt tcatcgtctg cgaagagaag aacatctcct
      481 gagagaaatg gattcacgga tagagcgaat gcatcgcgat cagcttccag agccagacct
      541 tcgatcttat ctatgcgaag aggtctcttc acacgcccag atatcaacgc gggaatccgt
      601 gattgcatta aacgagaaag tagaatgcca tgcagtacgc gaacagcttc acgatccggc
      661 atggccgtta agcgatcgtg atttgcctga tcgagaatat accctgggag aggagatgga
      721 agatcgtaga ctggttttct gttgagtgac agaaaattct tctggtctct aaacccgaac
      781 gcagcagatc cagtgtcgtc taacagccgc gccacgccgt tgagttgtat aagtgtttcc
      841 ggttcctggt aaaccatcgc aggaatgaat agaccccgac gagccgttgt ttccggaatt
      901 cgcacaaaga aatcgcgtgt aattgtagat cgattgtatt caattctgcc tgtatcagat
      961 ggcgttatgc ggcaatgccc gaacaaatcg cgctcgcata tcgtgggaag ttgtgcggtg
     1021 ccgtggacaa tgagctggcc cggattctga aggatttctg taatgcttac cggtacgtcg
     1081 tccgggtctg tccaggcaag gatggaattt cgcagatgta gaaattgtcc tatcatgttt
     1141 tcgtaggatc gaatcgtgat gtgcaggatc gtctgtactc cgaactttct gaaattggaa
     1201 ggcatcggga cgagcctttc gcttagaaga gtttcgtagt ccgctccctt atcgaataga
     1261 tgggagagca caaccggatt cccggatcgg ttcagtacgt cgcgcaccac aagtacgtat
     1321 gtgtccccgg cgcgcatggg cttttccggg atcacttcaa acaaacagat tgatgccctg
     1381 tgcaatcgca ccagaacggg aatgcttctt tcgatggccg tgttccagag gtaaaaggat
     1441 tcgccgggtg aaccagtaaa tcgatccgga tagtggtcgg agacgtcaca gggggtctca
     1501 agccagatgt gcgaaactgg gctgtagccg tccgttcggg ccagagccca caatccccca
     1561 tactggcccg ccgggaaagc ccagatcatg ccggatgcac ttctgttgga atgcgaaagc
     1621 attcgatcgt ccgggaaggg gaagttgcaa tccgagagag acagacggca gtcccccggc
     1681 ctggcatggg tggggaatga agccaggatg agaataactc tcagagctat aaaaacgatt
     1741 cgggtatttt gaatttttct tgaacgcagg gacggaaagc ctcgtctgat cttgtatcag
     1801 gaagccggag tggcttccca tcaagtttct attaggggac aggagaagtc tatgaagaaa
     1861 tggtatttga tcggggcgat tgccctgtcc agcatggttc tgtattgtgg cggtagcgga
     1921 aacgatgcac gtcgtgacgc gacgagcacc gggccagacg gttggatttt tgaaggttgg
     1981 gcttgcgcac cagacgcagc aaaagctctc ctcaagcaaa gtcctgctca gtactgccag
     2041 gatgacaagc caaaggactt tctgtatatg aagttcagcg cggctgcatc tcagaatgca
     2101 atcgacaaag gcatggttgc tatgaagcag tctacttgcc gcaaagcagc caaggatcag
     2161 atttcatctg acggtcttgc gaagatcctc ggtgaatacc tggaacaagc atccggtgtg
     2221 aaagacggcc agtccacagg tcaggtcatc atcgcgcaga accagggtat cattcgtggc
     2281 gtgtccatct acaattgctg ttctctcgac cagcagacag gtgtttgcgc gactgccgga
     2341 gctgcagaaa cctgggatca gtgccagtgt gtgggtgtta tgaaatatcc aggcggccag
     2401 aaaggtttcg aagctgctgc tgaaaaagca tccagctctg caaacccgta attcaaaact
     2461 cgaattgcga caaaagaagc cccggtctaa aagccggggt ttttttgtgt ccagggtcaa
     2521 cgaacgttag gcgaaaacac acgcaactgg aatgttcttg cagtaacttg tattgtgcaa
     2581 agttcttgtg acacgcgtgg ctagaattcc tctcctttgc attctgttcc ttggttcttg
     2641 tgcgcggatg aacgaggcca ctctagacct tccttctgac tggcgtattg tctccggatt
     2701 tcgagacgcg gcgaatcctc tcactgacgg aatgaaggtg cgacttcctg ttcgcccaaa
     2761 tgaaatcttc cctgatttta aaggttcgac agcgcactac acgctcgaaa ctacatttgc
     2821 gcttacgccc gacgaaatca cccgcctccc tgacccagct ttcttttttc ccatgctggg
     2881 ggaaggttac aatttgtatt tgaacgggaa ttccattgtt gagaaccttc ggcttcatcc
     2941 agacggagag ctgtctgcag tcactacagg tcgcggactg atcgttccat ttccgtcgca
     3001 cctgcttcgc gccggagaaa atcggctgtc cattcacctt gcaggattca cgcgaaggac
     3061 tgccctttca tccaattcgg tagactttgg tttacccgtg aagggcggct atcgcctcgc
     3121 ttcgcacaca actttgatgc gcgaaaattc ggaaatggca ctttacgcgt tttgcggcgt
     3181 ctacgcagtt tttgccttct acagtctcct ctattttgtt cgccgtccaa aacatattta
     3241 cactctctat ttcgcgctct tcacgtgtac ttattctgtg tacttgattt gtttgtccaa
     3301 tcgtgctttt gcttctgtgg cggatgttcg cacagtgatt tgcattgctt atgccgcaca
     3361 gcccgcggct ctcggattct ttgtagcatt tctcaatgcg tacaccggtg ccggatttag
     3421 aaaaacgaca cgcctcatga tcgcttttga ttccattctt gcgatcgccg cactctcctt
     3481 accgtatcca tacctgcaga gctggctcat cgtcttctat atggtgatgg cgccccagtt
     3541 gattcttgta ctgtacatca ttggagcggt cgtgaagagt cgcgcgcccg gtagcgcttt
     3601 tgctgctgca agtatttctc ttggtctcgg atttgttgta tgggatatcc tggatacggt
     3661 cttcttccgc acaagtatcc gcttgtcgcg atatgctcat tttcctgtga ttcttgcgat
     3721 gatgctgatg ctggtgaatc gatcgcttgc gtctatggaa gaagtggaga agttaaattt
     3781 ggagctttct tttgctacaa aggatctgga tcaacgcgtg caagaacgta cgcgccagct
     3841 cgaagcggcc atgagctctg caaagcgcgc tgacagagca aaaacaagtt tccttgcgaa
     3901 catgagccat gaaatcagaa cgccgcttaa cgttcttatt ggaatggggg attggctgca
     3961 cgaaagagaa gaagattctg aaaaacgtcg cgttcttgca agcatgctta cagccggcaa
     4021 tggtcttctc actctgattc aggaattgct ggatgttgcg cgcatagaat caggtacgat
     4081 acgccttgat tcagtgccct ttgacgcaga ggcgatcgcg caggatgtag ttcatttttt
     4141 tgaaggccgt gccaaagaaa aatcattgag cctcgagtat caatcccccg cgaaaagaat
     4201 tggaccgctt cgcggcgatc ccgccaggat tcgccaggtt cttgtgaatc tacttggaaa
     4261 ctcactaaaa tttaccgaaa caggaagtgt tcgtttgact ctgacccttc tcagtgagac
     4321 ttccgacacc tctaagattc gctttctggt gaaagacacc ggaccgggaa tcgctccaga
     4381 agacacccgg actatttttg aacgtttcgc gcagggagtt tcctccatga acaggaaaca
     4441 cgcaggcacc ggtttgggtt tgtctatcag tcaggactta ctgaaactta tgggcagtaa
     4501 gattgatctt acaacaaccg ttggccatgg ttctgaattc gcatttactt tagatttgcc
     4561 aaaatccgca gacgtgatcg cagaaatcgt acattcgccg gagttgccgg acgaacgtcc
     4621 gctgcggatc cttgttgcgg aagatgatga agtaaatcgc acactgcttt ccatgtattt
     4681 tgctcagtct gcgcataccc tggagttcgc gcataacggg ctggaagccg ttcgtgcctt
     4741 tgaatcacat ccttttgatg ttatcttcat ggacttgatg atgccggaaa tggacggaat
     4801 cgaagctacc attcggattc gaaaaattga gaccgagctt ctttcttctg gagtgcgcac
     4861 tcaaaagact ccgatccttg ctttgtccgc atccacatat gcggaggatg ttgctctaag
     4921 taagcaagcc ggttgcgacg agcatcttgc caagccggtg aaacgcgcga cgttattcgc
     4981 cgctctggca cgcgttacct cttagtcgca tggattgaaa ttcgcaatcg agtctggatg
     5041 caagcacatg cgaagaccca tcaatccgca agggtataaa gggttgcttt tcttcctttg
     5101 ataaggactc gtttttcgcg ggcagaagtt tcctgagaac cacagcctct cattgtaggt
     5161 cgatcgatga gaatgctgca accgagcttc tttgtcaggc tttccagcct cgcagcccgc
     5221 gcaaccgcat cccccacgat ggtccattca agtctgcgaa aggtgccaac gtttccttga
     5281 aatacttttc cggtagcaat tccaatcccg aatcccagct tgcgcgcagg ttcgctcgaa
     5341 ctttcttgcg cgatggactg gaagtactgc gcaacgcttt gtgcgcacga gatcgcagcg
     5401 cacgcctgtc ccggaagaaa agttgcaaca aggccgtcgc cggttagttt tgcaatgctg
     5461 ccgttcttct caaatataag atccatgagc gctatcataa ggctgttgag tagttccgca
     5521 aagggaatag cccccattat ttcggcgagg cccgtcgaat tacgcaaatc gaatatcagg
     5581 atcgtcgcga ctcctgtttg tccacttgca tctgcttcca tttctcccgt cagaatagat
     5641 gtcacagttt ccgctgagaa ataacgcgtg agtatttcct tttctcttgc gagttctgcc
     5701 tgaagctctt cgcttttttt tcggaaggca accgtttgca gcaagttctg aatacggatt
     5761 gcgagttctc ccaggtccag cggcttttcg agaaactcct ccgcaccctt tgccagactc
     5821 tccacgagca tctccgcagt tctatccgca gacatcaaca ctaccgaggc gtctttatca
     5881 atgagatgaa tttgttgcac caggtccagg ccggatccgt ccggcaggtg cacatcgagc
     5941 aaagtcagat cgggccggcg ttcatggatc aatcgtaacg catccgcgca cgtgggcgct
     6001 tcgcgtacgg agtagcgtag aatttccaga aactcgcgca gaagtctgga ttggatttca
     6061 tcatcttcga ccactagaat ttcaaaacga gactgatctg tgttctccac ttgcgtctc
//
LOCUS       DAOQJL010000189         6093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7328_length_6093_cov_4.098211, whole genome shotgun sequence.
ACCESSION   DAOQJL010000189 DAOQJL010000000
VERSION     DAOQJL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_6093_cov_4.098211"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLG78_09625"
     CDS             <1..209
                     /locus_tag="PLG78_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23416.1"
                     /translation="LSAAVGLFFGFPTLLIVFVAAACGSIFYSLVIQGSRNAPAPFGA
                     SLGFATLTAIPLQIVAQWAGFLQF"
     gene            complement(214..2385)
                     /locus_tag="PLG78_09630"
     CDS             complement(214..2385)
                     /locus_tag="PLG78_09630"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HNE23417.1"
                     /translation="MPKIELTDPYTTLPRGGYVLETSAGYLQVGSPPETIKDTMFLPR
                     STPQIFVLPNKFFHVTKGISVAELEFPIYYNHFLRQKKTYVVCTEEQREQLKIVLNEA
                     VFGPDVADLRSEYVQGEHTFGYPAMKAEMDYFRGNRKLEDLVRFVIFRDNRVRLNNVT
                     ITKKDDGNFVVEDTDWARTIEVPGEVGFNIIYDTGERLPEPYLPPVLGITCLGPSHGF
                     DTEDNTSGFLLWINHQGTMVDPPVNSTEWLRKSNVNPKLINTIILTHCHADHDAGTFQ
                     KILEEGRITIYSTETVMHSFVRKYHALTRIPMKELFTLFEFVPVIVGKSYMINGAEFR
                     FSYALHSIPSLSFEFNFQDMSFLYSSDHLNDPDKFKEMFEKGILTETRYRDLLNFAWH
                     HTIIYHEAGIPPLHTRVDYLKTLPEDIQKKITVYHIAKKDMPKDTKLTLATFGMENTV
                     YPPITPPKHEEAYRLLDVLSNIDIFRNFPISKSKEFLTLVEEEIFKRGDYIIRKNTMG
                     DKFYIIQSGNVKVEGMEQEEPNDGAFKRYGSYEYFGEASLILERPRSADVIAETDVIA
                     LTIEKSRFLNFIKGSDLLDKFAQLNEIRKTGTWEVLSQSRLLGGITSAQKTQLELIMD
                     RKSFRAGSYLIEQGRNFYEAFIIKHGTVEVLSNGRVVEKLGRGDFCGEIFCLQKESPS
                     TLTFRAVSDIDVYAIDRPRLVHYIADNPGVYMRLNYVYGAS"
     gene            2407..2739
                     /locus_tag="PLG78_09635"
     CDS             2407..2739
                     /locus_tag="PLG78_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23418.1"
                     /translation="MFRRRSSFFNLKKSLLALVFAFGCASDSRDPRIAAIVSMSAGHD
                     HGTNVTYRLLHSGMRTFALLENQGHSHAFTLTLEQAALIELGFPVTVESTSTNTHTHT
                     VVLRKQDL"
     gene            complement(2748..3059)
                     /gene="trxA"
                     /locus_tag="PLG78_09640"
     CDS             complement(2748..3059)
                     /gene="trxA"
                     /locus_tag="PLG78_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE23419.1"
                     /translation="MAVVALKDDSFSQDTKEGLVLVDFWAEWCGPCRMVAPIVEELSS
                     EMPQVKFAKVNVDENQRIAQTLGITAIPTLVLYKDGQPVDRVVGLLPKPQLKNFINKH
                     V"
     gene            complement(3113..3919)
                     /locus_tag="PLG78_09645"
     CDS             complement(3113..3919)
                     /locus_tag="PLG78_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23420.1"
                     /translation="MGVTKEQKNLYNERVKSYKQGLDEIKKEMAMVKGMGKSPTLQPY
                     ATIRLAIESLKNATIHAQMSRLSYRILKIRRDEVLADAKKDIFNSLTDLLKLVGSDVD
                     ESLTENKERLPKIAAMTPRQKLELLKGFRSVTDYVQEAMGPSHKMRWSFPELHYKIAV
                     LGKNIMDFKEYERSKDPTEEHYQDRRALLQFIVDQCHFAAQEYRSKHELSTKEVTDLQ
                     SVRKMFELIKKIQSMTGNRTELEKITTSLDSVNEKIEAMMAEKKDKKKKI"
     gene            4022..4312
                     /gene="tatA"
                     /locus_tag="PLG78_09650"
     CDS             4022..4312
                     /gene="tatA"
                     /locus_tag="PLG78_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNE23421.1"
                     /translation="MVLFLTNSPFDWVIILVIVLILFGGRKIPALAKDLGGGIREFRQ
                     ALLNPNQTQTTQMIEHQPQPAETYTPPPAAPRKRAAAKPAARKPAKKSRARS"
     gene            4312..5148
                     /gene="tatC"
                     /locus_tag="PLG78_09655"
     CDS             4312..5148
                     /gene="tatC"
                     /locus_tag="PLG78_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNE23422.1"
                     /translation="MAGRKRATRARAPIQPPPTPAPAPEELPDPREGVMTLGDHLEEL
                     RRRILWSLGILAIFSIAAGFFISDIHRYISEPYHRATGLNMMLGSAMDALNTMIQLSV
                     AVGFAAGLPVVIFMLFEFVTPAMERRTAIYARISMVFSCLLFWGGVLFCWFYIFPLSV
                     EMMFKFLLPPNTQALISLDKYYSFFFILHIGTGVTFQLPLLIVILGALGVVPISLHRR
                     AWRFVIVGMFLLSMAVTPGDPVSLFVVALPLCVFYAIAVAIVYMIERNREKAAARDAA
                     EA"
     gene            complement(5155..>6093)
                     /gene="clpX"
                     /locus_tag="PLG78_09660"
     CDS             complement(5155..>6093)
                     /gene="clpX"
                     /locus_tag="PLG78_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNE23423.1"
                     /translation="GSGKTLLAQTLAKMLKVPFAIVDATALTEAGYVGEDVENILLRL
                     IQNAENDVKRAEIGIIYIDEIDKISRKGDNPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQDFITLDTRNILFICGGAFVGLDKIIERRLGSRTIGFNRDEEAARKAGE
                     KSEILANVLPDDLMKFGLIPEFVGRLPIVATLQDLDVETLKKIFMEPRNSLLKQYQKI
                     FEIEGVKLKFTDEAITRIAELAIKREAGARGLRSIVEHQMMDLMYDIPSRDDVESVAI
                     TPGVIESGDSPVLSFKKEGAKEKEKASSSKKQEKLA"
BASE COUNT         1419 a         1563 c         1432 g         1679 t
ORIGIN      
        1 ctctctctgc cgcggtcggt ctgtttttcg gattcccaac tctactgatt gtgtttgttg
       61 ccgccgcatg cgggagcatc ttctacagtc tggtcatcca gggatctaga aatgcgcccg
      121 ccccgtttgg cgcaagcctc ggctttgcta ctctgactgc gattccgcta caaatcgtgg
      181 cgcagtgggc aggctttctg caattctagt cgatcaggaa gcgccgtaga catagttcaa
      241 gcgcatatac acacccggat tgtctgcgat gtagtgtaca agccgtggcc tgtcaatggc
      301 gtatacatct atgtccgaga cggcgcgaaa cgtcaaagta gatggcgatt ccttttgcag
      361 acagaagatc tctccgcaga aatctcctct gcccagcttc tccactacgc gtccgtttga
      421 tagaacttcc acggtcccgt gtttgatgat aaacgcttcg tagaaattac gtccctgctc
      481 gatcaaatac gaacctgcac ggaagctctt acgatccatg atcaattcaa gctgcgtttt
      541 ctgcgcggaa gtgatcccgc caagaagacg agactgagag agaacttccc aggttccggt
      601 ctttctgatt tcattgagct gtgcgaactt atcgagtaga tccgaaccct taataaaatt
      661 taggaatctt gacttttcaa tggtaagagc gatcacatcc gtctctgcaa ttacatccgc
      721 agagcgagga cgctccagga tcagtgaggc ttccccgaaa tactcgtaac ttccgtagcg
      781 tttaaacgca ccatcgttag gttcttcttg ctccattcct tccactttta cgtttccaga
      841 ctgaatgatg tagaacttat cccccattgt gtttttgcgg atgatgtagt ccccgcgctt
      901 gaaaatttcc tcttctacca gagtgagaaa ctcctttgat ttggagatgg ggaagttgcg
      961 aaagatatca atgttggaaa gtacatccag cagcctgtac gcctcttcat gttttggcgg
     1021 agtgatcggt gggtataccg tgttctccat gccaaaggtg gccagagtga gcttcgtgtc
     1081 ctttggcatg tccttcttgg caatgtgata taccgtaatc ttcttctgga tgtcctccgg
     1141 aagagtcttc agataatcca cgcgagtgtg tagaggtggg attccagcct cgtgatagat
     1201 aatcgtgtga tgccatgcaa agttcaaaag gtctctgtaa cgcgtttctg ttagaattcc
     1261 cttctcaaac atttccttga atttgtcagg atcattgagg tggtcggagg aatagagaaa
     1321 agacatatcc tgaaaattga actcaaaact caggcttggg atagaatgca gtgcgtagct
     1381 gaatctgaat tcagctccgt tgatcatata gctcttgcct acaatcacgg gaacaaattc
     1441 aaaaagcgta aacagctctt tcattggaat gcgagtcaat gcgtggtact ttcgcacaaa
     1501 ggagtgcatc acggtttcag tagagtagat tgtgattctg ccttcttcca ggatcttctg
     1561 gaaagttcca gcatcatgat ccgcatgaca atgagtgaga atgattgtgt tgatgagctt
     1621 tggatttacg tttgatttgc gtagccactc tgttgaattc acgggtggat ccaccatagt
     1681 cccttgatga ttgatccaga ggaggaaccc acttgtatta tcttccgtat caaatccgtg
     1741 acttggacca agacatgtaa ttccaagtac aggaggtaga tacggctcag gaagacgctc
     1801 acctgtatcg taaatgatgt taaatcctac ttcaccgggc acttcaatgg tgcgcgccca
     1861 gtccgtatct tccaccacaa agttgccgtc gtctttctta gtgatagtga cattgttgag
     1921 tcgcactcga ttgtcccgaa agatgacaaa tcgaaccaga tcctcaagct tgcgattgcc
     1981 ccggaagtaa tccatctcag ctttcatcgc agggtaacca aacgtatgct ctccctggac
     2041 gtactctgat ctaaggtctg ctacatctgg accgaacacg gcctcattca gcacaatctt
     2101 cagctgctca cgctgttcct ctgtgcaaac tacgtacgtt ttcttctggc gcagaaagtg
     2161 attgtagtaa atgggaaact caagctccgc tacagaaatc cctttggtta cgtgaaagaa
     2221 cttgtttggc agaacgaaaa tctgaggagt gctccgtgga aggaacattg tgtccttaat
     2281 cgtttccggt ggtgagccga cttgcagata acccgcggat gtctcaagga cgtaaccacc
     2341 acgtggaaga gttgtgtacg gatctgtaag ttcgatttta ggcatgctct tccttttgac
     2401 aggcctatgt ttagaaggcg aagctccttt ttcaatctaa aaaaaagcct tctggccctt
     2461 gtcttcgctt tcggatgcgc gagcgacagc cgggatcctc gcattgctgc catcgtaagc
     2521 atgagcgccg gccatgatca cggaaccaat gttacataca ggctgcttca ttccggaatg
     2581 cgaacgttcg cccttcttga aaaccaggga cacagccacg cattcacgct gactctggaa
     2641 caggctgctc tgatagaact tggatttccg gtgactgtgg agagcacttc aacgaacacg
     2701 catactcaca cagtggtcct gcgcaagcag gacctctgaa cggtagacta gacgtgctta
     2761 ttgatgaagt tctttagctg tggttttgga agcaaaccaa cgacacgatc caccggctgc
     2821 ccgtccttat agagaacgag tgttgggatg gctgtgatgc ccagagtttg cgcgatgcgc
     2881 tgattttcat ctacgttcac cttcgcaaac ttcacttgag gcatttcgct ggaaagttct
     2941 tctacaatag gggcgaccat gcgacagggg ccgcaccact ctgcccagaa atcaacgagg
     3001 acaaggccct ctttcgtatc ctggctaaaa gaatcatctt tgagtgctac aactgccatg
     3061 ggtcctccta caagactgat tccataaacg gggggcggaa gggacgcgga aactaaattt
     3121 tcttcttttt atcctttttc tcggccatca tcgcttcaat tttttcgttt acggagtcca
     3181 gagaagttgt tatcttctcg agttccgtcc tgtttccggt catgctctga atctttttga
     3241 tcagctcaaa catctttcgc acgctctgca aatcggttac ttctttcgtg ctgagttcat
     3301 gcttggaacg atattcctgt gccgcgaaat ggcattgatc cactatgaac tgcaagagcg
     3361 cccgcctgtc ctggtagtgc tcttccgttg gatctttaga tctctcgtat tccttgaaat
     3421 ccatgatgtt ttttccaagc accgcaatct tgtagtgcaa ttcaggaaaa gaccatctca
     3481 tcttgtgact cgggcccatc gcctcctgaa catagtccgt cacagagcga aatcctttca
     3541 gcaattcaag cttctgcctg ggcgtcatcg cagcgatctt cggtagcctc tctttgttct
     3601 ccgttagact ttcgtccaca tccgaaccca cgagcttgag caaatctgtg aggctgttga
     3661 atatatcttt cttcgcatcc gcgagcactt catcccggcg gattttcagg atgcgatacg
     3721 aaaggcgaga catctgcgca tggatggtcg cgttcttcag actctcgatg gccaggcgaa
     3781 tggttgcata gggctggaga gtgggagatt ttcccattcc tttcaccatg gccatttcct
     3841 tcttgatctc gtcgagaccc tgtttatagg atttcaccct ttcgttgtat aagttcttct
     3901 gctctttggt gacgcccatc tggtttaaac ttcggtccta tcctacccgc aaaaaaggaa
     3961 atgcaagtga aaatcacttg ttttcgcgcg attcatggat tgtttggacc caggcaggca
     4021 tatggtactt tttttaacga acagcccctt cgactgggtc atcattctag tcattgtact
     4081 catcttattc ggtggccgca aaatcccggc tctggcaaaa gatctcggtg gcggcattcg
     4141 tgaatttcgt caggctctcc tgaatccaaa ccagacacaa accactcaga tgatcgagca
     4201 ccagccacaa ccggcggaaa catacactcc tccgcctgca gcaccccgga aaagggcggc
     4261 tgcaaagccc gcagctcgaa agccagcaaa gaagtctaga gcaagatcct gatggcagga
     4321 cgcaagaggg ccacgagggc ccgcgccccg atccagccac ctcccacgcc agcgcctgcg
     4381 ccagaagaac tgccggatcc acgcgaaggc gtgatgactc tgggcgatca cctggaagaa
     4441 ctcagaagaa gaatcctctg gtctctgggc atccttgcta tcttttctat tgctgccggc
     4501 tttttcattt cggacattca ccgctatatc tctgagccgt accatcgcgc caccggactc
     4561 aacatgatgc tcggaagtgc catggacgct ttgaacacga tgatacagtt gagcgtggct
     4621 gttggatttg ctgcgggctt gcctgtcgtc attttcatgc tctttgaatt tgtaacacct
     4681 gcgatggaac ggcgaacagc gatctacgca cggataagca tggttttttc ctgcctgctt
     4741 ttctggggcg gagtgctctt ctgctggttc tacatctttc cactttctgt ggagatgatg
     4801 ttcaaatttt tgctgccgcc caatacacag gcgctgatca gtctggataa atactacagc
     4861 ttctttttta ttctgcacat aggcacggga gtaacattcc agctccctct actcatcgta
     4921 attctaggag ctctcggagt cgttcccatc agccttcatc gccgcgcatg gcgatttgta
     4981 attgttggta tgtttcttct atcgatggcc gtgacaccgg gagaccctgt gtctctcttt
     5041 gttgtagctc tacctctttg cgttttctat gccattgcag tggcgatcgt ctacatgata
     5101 gagagaaaca gagagaaagc agcggcgcga gatgcagcag aagcctgaca gacgtcaggc
     5161 gagcttctct tgtttcttag aagaggatgc cttttccttt tctttcgctc cctctttctt
     5221 gaaactgagc acgggcgagt ctccggactc aatcactcca ggagtgatgg caacactttc
     5281 gacgtcatcg cgcgaaggaa tatcatacat caaatccatc atctggtgtt ctacaatgga
     5341 tcgcagtcca cgagctcccg cctctctctt gatggcaagc tccgcaatgc gagtgatggc
     5401 ctcgtctgtg aatttaagtt tcacgccttc gatttcaaaa atcttctgat actgcttgag
     5461 gagagaattg cgcggctcca tgaagatttt cttcagagtt tctacatcca gatcctgaag
     5521 agtggccaca atcggtaaac gacccacgaa ctccgggatc aaaccgaatt tcataagatc
     5581 atctggaaga acattggcca ggatttcgct tttctctcct gctttgcgcg ctgcttcttc
     5641 gtcccgattg aaaccaatcg ttctgcttcc gagcctgcgt tcaatgatct tgtccagacc
     5701 aacaaatgca ccgccgcaga taaaaagaat attgcgagtg tccagagtga tgaagtcttg
     5761 atgaggatgc ttgcgtcctc cttgaggagg aacgttggcc acagtgcctt ccaggatctt
     5821 gaggagggct tgctgaaccc cttctcccga aacatcgcgc gtgatagacg ggttgtctcc
     5881 tttgcgagag atcttgtcta tttcatcaat atagatgatt ccaatttcag cgcgcttcac
     5941 atcattctca gcgttctgaa tcaagcgaag aaggatgttc tccacatctt cgcctacgta
     6001 gcctgcttct gtcagagcag tagcatccac gatggcaaac ggaaccttga gcatttttgc
     6061 gagagtttga gccaggagag ttttgcccga acc
//
LOCUS       DAOQJL010000190         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7378_length_6063_cov_4.681425, whole genome shotgun sequence.
ACCESSION   DAOQJL010000190 DAOQJL010000000
VERSION     DAOQJL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7378_length_6063_cov_4.681425"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLG78_09665"
     CDS             complement(<1..978)
                     /locus_tag="PLG78_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE23424.1"
                     /translation="MRIATLALFFALVNCTRIQNPSEPLPASPPAPQKFDIPSRSYWP
                     TAGWRTSKPEEEGLKPEAIAKLNQYLFTRTGDDLDRNGVRTDGALIIRGGKIVYERYA
                     RGYDENRPHLIWSSAKSFISALIGIAVKEGKLSLSDSASKYFAEMKDGQKSEIKVEHI
                     LQMSSGLAWNEGYEASPLDSSVIAMLYTAGRTDMARYTASYPLRYRPGTRWYYSSGDT
                     NMLSAILRKTMSEEEYQNYPWAKLFEPLGMKNMTFERDGSGTFVGSSYIYGSPRELAK
                     FGFLYLNDGVWDGKRILPEGWVTLSRTMAPAFYAHPEREDAWDGNYGAQW"
     gene            complement(1028..1984)
                     /locus_tag="PLG78_09670"
     CDS             complement(1028..1984)
                     /locus_tag="PLG78_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE23425.1"
                     /translation="MTALKIKSAWFYISFAFALTACSGQIKNGERITKDPLILKDQEA
                     TSSQPLPWKMVRTPEAAALNDSGVEAVHDLRLDDAEKLFEDSIQKSPLYGLAYLNLAR
                     LYWVAEEENNSSKVFARLAVSGISYEDLLKTADRLYEYSRTRESVALLEQLSQIPQAG
                     HLPSLRLGNYYLGQALYAQADAHFDRVLAKKPGNADALFARGFIRFQANDFERAASFL
                     EAAKTNGSQEPQLCRLYLQSLFRTNKMEKAKTEQANCRGTDPDLAEIKIRILLVLNPF
                     EDVSRIVAELKQEDAAALEMRVFGSNNRSALRQIQKELELGY"
     gene            complement(2050..2949)
                     /locus_tag="PLG78_09675"
     CDS             complement(2050..2949)
                     /locus_tag="PLG78_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23426.1"
                     /translation="MKQKLSLITFAFTLISCSTIQTTENEDLQKQEREKLEQQTREDQ
                     TRADQKKRFAEEEKKRLELLETAKKDREKLDAQEKEKARMAEMQRREEARKAEEKRAA
                     DEQKRTDEKKTAEMQIADQKKEEIKKQEEAARAAAEERERQRILEEQRLAEERRKAFE
                     ARQNRLDQIDPDRKAFFRIEMQNKVGSLSCRRDSVIIYRNKRPLQFQGYTMAALPDAD
                     IDSPFSFGGKDLADALRGKKGHFVALEDRSTTRPLLCDGRNGSFRIIDMDSDGEGLWL
                     GLEMENSNGEFYKIYTESVPLGR"
     gene            complement(2946..3941)
                     /locus_tag="PLG78_09680"
     CDS             complement(2946..3941)
                     /locus_tag="PLG78_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23427.1"
                     /translation="MLRSVIVLVAVCAATGPLLAGNGTLLFNQAYKLEETDPSGAVEI
                     YHSAIQAGLTTELHRAARWRLFFIFKDQENFPEALRESEYLPASARTQLVEYIRERYS
                     PSKTAFESYLSGIRLIISSDSEKRNQGAVQLEKIYSTSDRLFQKRILLDMSEHGSAER
                     ALNLIRSSGKDTASVLRECDLLIYLGRLYEAEQSISKLLANQKDDFSTEEKMRLVYLL
                     GRARRDSGPDVEMFRISEKYATGEEKMRMRALAAYALLRHDYPVQARALMKDNSSSDP
                     DVQLLDLVLRAEIDRDPQALRTLQGKKESLKSTVRKKKDAYLAQRALRLIAGVEH"
     gene            complement(3972..4502)
                     /gene="thpR"
                     /locus_tag="PLG78_09685"
     CDS             complement(3972..4502)
                     /gene="thpR"
                     /locus_tag="PLG78_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNE23428.1"
                     /translation="MGAVRLFVAIPIPEEVQDDLGGLTGSLAGVKWTDPQNIHLTLHF
                     LGEDFEPEEIHSALASVHSGPFPVEVKGVGRFLHNQGGAIWAGVLPSPQLMELHARIG
                     KALRAVSVPTEKRAYQPHITLGRVKNEPEHRIQDYILHHQDFHTSFKAVSFALFSSRL
                     RPDGPIHSIDMEYEFD"
     gene            4628..5413
                     /locus_tag="PLG78_09690"
     CDS             4628..5413
                     /locus_tag="PLG78_09690"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNE23429.1"
                     /translation="MEYYLICALIFGVAYLVNATYISIFYHRGLTHSAIELKPGLRKF
                     VVASGPWVMGIDAKTWCTMHRLHHRHSDKESDPHSPLNSSIPRVIVEQLKSYERIMRG
                     LIARKKQYTSEVPDLDFNVHWLYRNKLWWLPYALHLAISVGLSFALGGWLVGLAFYSG
                     MVTHPIQGWLVNSFGHSMGYQTFDNGDNSRNNNIVSWLVMGEGYQNNHHKHPRSPKFS
                     YRWFEVDYGYRICQAMQALGMITIEKDYLIRVVERRELEERAA"
     gene            complement(5431..>6063)
                     /locus_tag="PLG78_09695"
     CDS             complement(5431..>6063)
                     /locus_tag="PLG78_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23430.1"
                     /translation="LMLHGVGNWGNFWRPGATSTSIRKRYTINAGAGQYEVSWQFLDQ
                     LQVALVGAGASGRLGHEPNASSSEFRTDQFRTNSSAFQFTSIALDSSGGYTIFPGGKL
                     TGIVAHGIEFRSTIFNVLQADFSAYLIQLYRSPVVDYNAYYTRYPALHRSDRRLGKEY
                     DAKFAWRFLSDLTLEAKLAYFDADDGYRIINDVVYGDDMAEIQISATQKF"
BASE COUNT         1331 a         1508 c         1518 g         1706 t
ORIGIN      
        1 ccattgtgct ccgtagttgc cgtcccaggc atcttctctt tctggatgtg cgtagaaagc
       61 cggggccatc gtacgcgaaa gagtgaccca tccttctggc aaaattcttt ttccgtccca
      121 gacaccgtcg ttcaaataga ggaatccaaa tttcgcgagc tcccgcgggg acccatagat
      181 ataagatgag ccgacgaaag ttccagaccc gtctctttca aaagtcatgt tcttcattcc
      241 caggggctca aagagttttg cccatggata gttctggtat tcttcttcgc tcattgtctt
      301 gcgaagaatg gcgctgagca tattcgtatc tccgctcgaa tagtaccatc tggtaccggg
      361 acgatatctc agaggatagc ttgccgtata acgggccatg tctgtgcgtc cggctgtgta
      421 gagcatggcg atcacggatg aatcgagagg agatgcttca tatccttcgt tccatgcaag
      481 cccggagctc atctggagaa tgtgttctac tttgatttct gacttttgtc cgtctttcat
      541 ctccgcgaaa tatttggatg cagaatcaga aagcgagagc ttcccttctt tcactgcaat
      601 tccaatcagt gcgctaatga aacttttcgc agaagaccag atcagatgcg gacgattttc
      661 atcatatcca cgtgcgtaac gttcgtagac gatttttcct ccgcgaataa tcaaggctcc
      721 atctgtgcgc actccatttc tatccagatc gtcgccggtg cgtgtgaaca aatactgatt
      781 gagcttcgcg attgcctccg gcttcagtcc ctcctcttcc ggtttagagg ttctccatcc
      841 ggcggttggc cagtaggaac gagatggaat gtcgaatttc tgcggtgcag gtggcgatgc
      901 aggaagtggc tcagaagggt tttgaattct ggtgcagttt acaagtgcga aaaacagggc
      961 cagagtggcg atgcgcatag tccgattgca acgataggaa aggacgctac aagcaaaaaa
     1021 accccaatca ataacccagc tctagttctt tctgaatttg ccgcagagcg gatcgattgt
     1081 tggatccaaa gacgcgcatt tcaagggccg cggcgtcttc ctgtttcaat tccgcgacga
     1141 tccgggaaac atcctcgaac ggatttagca caagcaatat gcgaatcttg atttccgcca
     1201 gatctggatc agttccgcgg cagttcgcct gctctgtttt tgccttctcc attttgtttg
     1261 tgcgaaacag ggactgcaga taaaggcgac agagttgagg ctcctgcgat ccatttgtct
     1321 ttgccgcctc aaggaagctt gccgctcttt caaaatcatt tgcctgaaaa cgaatgaagc
     1381 cgcgtgcgaa aagcgcgtcc gcgtttcccg gtttttttgc gagaacgcga tcgaagtggg
     1441 catctgcctg cgcatacaag gcctgaccca gataataatt cccgagccgc agagatggga
     1501 ggtgtcctgc ctgcggaatc tgactgagtt gttccagcaa tgctacggat tctctggttc
     1561 gggaatactc gtagaggcgg tccgccgttt tgagcaggtc ttcgtaagag attccggaca
     1621 cagccaggcg agcaaagact tttgacgaat tgttttcttc ttctgccacc cagtagagtc
     1681 gtgctagatt tagatacgca aggccgtata acggcgattt ttgaatggaa tcttcaaaca
     1741 acttttctgc gtcatccaga cgcaaatcat ggactgcctc cacaccacta tcattgagcg
     1801 cggctgcctc cggagttcgc accattttcc acggcagcgg ctgactgctg gtggcttctt
     1861 gatctttgag aatgagcgga tcctttgtga tgcgttcgcc gttcttgatc tggccgctgc
     1921 aagcggtcaa cgcgaaggca aagctaatgt agaaccaggc gctttttatt ttcagtgcgg
     1981 tcacgggttc atgcttggtt tgtttgcaga tgggtcaatt cgcttccagg gactattgcg
     2041 catttatctt catcttccga gcggaacgga ctcggtgtat attttgtaga attctccatt
     2101 tgaattctcc atctcaagcc caagccacag tccctctcca tcagaatcca tgtcgatgat
     2161 gcggaacgac ccattccttc catcgcataa gaggggcctt gtcgtggatc tgtcctcgag
     2221 tgctacgaag tgtccttttt tgccgcgcaa ggcgtccgcc agatcttttc cgccaaatga
     2281 aaagggagaa tctatatctg cgtcgggtag agctgccatg gtatagccct gaaattgtaa
     2341 cggtcttttg tttctgtaga tgattacact atcgcggcgg caggaaagac ttcctacttt
     2401 gttttgcatt tcaatgcgaa agaatgcttt tctgtccgga tcaatttgat cgagtcgatt
     2461 ctgtcttgct tcaaaggctt ttcttctttc ttctgccagc ctttgttctt ccagaatcct
     2521 ttgtctttcg cgctcttctg cggcggcccg tgcagcctct tcttgttttt tgatttcttc
     2581 cttcttttga tctgctattt gcatttctgc ggtttttttc tcatccgttc tcttttgttc
     2641 gtcggcagct cttttttctt ctgctttccg tgcttcttcg cgtctttgca tttctgccat
     2701 cctggctttc tctttctcct gtgcgtccag tttctctctg tctttctttg cagtttccag
     2761 tagttcgagc cgtttctttt cttcctccgc gaacctcttc ttctgatccg cgcgtgtttg
     2821 atcctctcgc gtttgttgtt cgagtttttc tctttcctgt ttttggaggt cttcgttctc
     2881 cgttgtctgg atcgtgctgc aagaaatcag cgtgaacgcg aaagtgatca gcgagagctt
     2941 ctgcttcaat gttctactcc cgcgatcagg cgcagggcgc gctgcgcaag ataggcgtct
     3001 ttcttttttc taacggtgga tttgagtgac tcctttttcc cctgcagtgt gcgcaatgcc
     3061 tggggatctc tgtctatttc tgcgcgaagc accagatcaa ggagttgcac gtccggatcg
     3121 ctcgaagagt tgtctttcat caatgctcgc gcctggacag gatagtcgtg gcgtaacaac
     3181 gcgtaggcgg ccagggctcg cattctcatc ttctcttcgc cggttgcgta tttctctgag
     3241 atgcgaaaca tttctacgtc cgggccagaa tctcggcggg cccgcccgag gagatagacg
     3301 agcctcattt tctcttccgt ggaaaagtca tctttctggt tggcgagcag tttcgaaatg
     3361 ctttgctctg cttcatagag tctgccaaga tagatcaaca aatcgcattc acgaagaacg
     3421 ctggccgtat ctttgcctga agaacggatc aaattcaacg cacgttccgc agaaccatgc
     3481 tcagacatgt ccagcaggat tctcttctga aaaagacgat ctgacgtgga gtaaattttt
     3541 tccagctgca cagcgccctg attccttttt tccgagtcag aactaatgat tagccgtatg
     3601 ccgctcaagt acgattcaaa agccgtttta gatggcgaat aacgttcgcg tatgtactct
     3661 acgagctggg tgcgagcgga ggccggcaaa tactcgcttt cgcgcagggc ttccggaaaa
     3721 ttttcttgat ctttgaaaat gaagaagagt ctccatcgcg cggcgcgatg cagctctgtg
     3781 gtaagtcctg cctggattgc agaatgataa atttcaactg ctccggaagg atctgtttct
     3841 tccagtttgt acgcctgatt aaatagaaga gttccatttc cagccagtag cgggccggta
     3901 gctgcgcaaa cggcgacgag aacgatgacg gagcgcagca tggctcgatt tgaaatcggg
     3961 gccggataac ttcaatcgaa ttcgtattcc atgtctatgg aatggatcgg gccatccggt
     4021 ctcagtctgc tcgagaacaa tgcgaaagac acggctttga aggaagtgtg aaagtcttga
     4081 tgatgcaata tgtaatcctg gattctgtgt tctggttcgt tcttcacccg gcccagcgtg
     4141 atgtgaggtt gataggcgcg tttttccgtt ggaacagaaa cagcgcgaag agccttgccg
     4201 attctcgcgt gcaattccat aagttgcggc gatggtagaa cgccggccca gatggccccg
     4261 ccctggttgt gcaggaatcg acctacccct ttcacttcca ccggaaaagg accggagtga
     4321 acggaggcaa gagcggagtg gatttcctcg ggttcaaagt cctctccgag gaaatggagg
     4381 gtgagatgga tgttctgtgg gtcggtccat tttacgccgg cgaggctgcc tgtaagccct
     4441 cctagatcgt cttgaacctc ttccggtatg ggaatggcga caaatagacg cacggcgccc
     4501 atttttccgc attgggcaga attctgcact cgatttaggt ccaggatgag gaaagggctt
     4561 gtcagcgccg gagtaagaaa aaacatggtg tatatctaac catgttagat atgcacggag
     4621 ggttatcgtg gaatactatt tgatttgtgc cttgattttt ggcgtcgcgt atctggtgaa
     4681 tgcgacctat atttctatct tctaccatcg cggattgact cacagcgcca ttgagctcaa
     4741 gccgggactg cgcaagttcg tcgtggcaag cggtccgtgg gtgatgggaa tcgatgcgaa
     4801 gacctggtgc acgatgcaca gacttcacca tcgtcactca gataaagaaa gcgatccgca
     4861 cagtcctctt aactcaagca taccacgcgt gatcgtggag caactcaagt cctacgaacg
     4921 gatcatgcgc ggactcatcg caaggaaaaa gcaatacacc tccgaggtgc ctgaccttga
     4981 tttcaacgtg cactggttgt atcgcaacaa gctctggtgg ttgccttacg ctttgcacct
     5041 tgcgatttca gttggtcttt ctttcgctct gggcggatgg ctggtaggcc ttgcgttcta
     5101 ttctggaatg gttacccatc caattcaagg ctggctcgta aattcttttg gtcattccat
     5161 gggataccag acgtttgata acggagataa ctcaagaaac aacaatattg tttcatggct
     5221 tgttatgggt gaaggatatc agaacaatca ccacaaacat cctcgctctc caaagttttc
     5281 ctatcgctgg tttgaagtgg attatggtta tcgcatttgc caggcaatgc aggccctggg
     5341 tatgattacc attgagaagg attatttgat tcgcgtagta gaaagacgcg aactggaaga
     5401 gcgcgctgcc tgattgaggg ctgtgttcga tcagaatttt tgcgtggccg agatttgaat
     5461 ctcggccatg tcgtctccat agacaacatc attgataatt ctatagccat catctgcatc
     5521 aaagtaggcc agttttgcct ccagagtcag atccgacaaa aatctccacg cgaacttcgc
     5581 atcatattct ttgcccagtc ttctatctga tctgtgcaat gccggataac gggtgtagta
     5641 cgcattgtag tccacaactg gcgagcggta caattgaatc aaataagcgg aaaaatctgc
     5701 ctgcagtaca ttgaagatag tggatcgaaa ctcgattccg tgcgctacga ttccggtcag
     5761 ctttccgcct ggaaagattg tgtatcctcc ggaagaatcc agggctatag aagtgaattg
     5821 gaatgcagat gaattcgtgc gaaactgatc tgtgcgaaat tcgcttgagc tggcattcgg
     5881 ttcatgcccg agtctgccgg atgctcctgc tcccactagc gccacctgaa gttgatcgag
     5941 gaattgccac gaaacctcgt actgtcctgc tccagcattg atcgtgtacc tttttcttat
     6001 ggacgtcgag gttgccccgg gacgccagaa gtttccccag ttcccgacac cgtgcaacat
     6061 aag
//
LOCUS       DAOQJL010000191         6033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7429_length_6033_cov_4.381733, whole genome shotgun sequence.
ACCESSION   DAOQJL010000191 DAOQJL010000000
VERSION     DAOQJL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7429_length_6033_cov_4.381733"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLG78_09700"
     CDS             <1..424
                     /locus_tag="PLG78_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNE23431.1"
                     /translation="AKNHPDTYDIIIVDSSDPIGPAEVLFQFPFYESCRNALKSDGIL
                     ATQAENFLLHAKIIKELLSFGKKLFPISRYYYTQVPTYPGGMIGFTFFSRKHAHDELV
                     DKRFAESGIKAEEFRFWSVDFHKKAFTLPGATMRDFYS"
     gene            complement(686..2497)
                     /gene="guaA"
                     /locus_tag="PLG78_09705"
     CDS             complement(686..2497)
                     /gene="guaA"
                     /locus_tag="PLG78_09705"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNE23432.1"
                     /translation="MSDNRKIGVVDFGGQYAHLIASRIRRLGALAQIADPETLTPSEA
                     RAQYSGLIYSGGPSSVYEPGAPASDPALLSAGLPVLGICYGHQLMMQQLGGEVRNSGH
                     HEFGRAILKVKSDVSVLRGENNSVSAVWMSHGDEVTRMAPGFVGIAHTDDCENAAVFN
                     AEKNLTGIQFHPEVQHTEHGDQYLLNFIRMCGLENSYRIGDFLSKEKEALARSIGDRS
                     VFFLLSGGVDSTVAFTLLSEILPADRLYGLFIDTGFMRKDEGIEVGTALRAHGIQLNI
                     EDARKRYATSLAGVVDPEKKRRIIGDLFLEIQADCVRRLNLDPEHWLLGQGTIYPDTI
                     ESGGTKKSHLIKTHHNRVPAIQRLIEEGKVVEPIRDLYKDEVRELGRLLRLPEKLVDR
                     HPFPGPGLAIRLLCSDADNADFRSAAALLAEHNQPLLKDIECTILPIKSVGVQGDQRT
                     YAHPALVYEREGDTARLHEIARALPNFVREVNRVLRFVAGNRPEMVRKAELSADRVER
                     LREADHVVNSFLHEKNIYGEIWQFPVVLVPLARKGVEEDAVILRPVVSLDAMTVSPYE
                     IKRDQLKDLSNRLLAISGIGSILYDLTTKPPGTIEWE"
     gene            complement(2503..3021)
                     /locus_tag="PLG78_09710"
     CDS             complement(2503..3021)
                     /locus_tag="PLG78_09710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE23433.1"
                     /translation="MLNYIRKNWSSILLFAFVIGFVLYRKIPLWENERGLEGQSLPHG
                     SLRTLAGSEIDLSSRKEKVILVSFWATWCMPCRVEMPMLVSLRRELDGQSFEIFAVTD
                     EDPITVAHFIEEHPVNFPIVLDRGPLQQAFRIQVFPTLVQFKDGKLVSVSNGVDWFLK
                     FKIRHAVTGNYF"
     gene            complement(3022..3774)
                     /locus_tag="PLG78_09715"
     CDS             complement(3022..3774)
                     /locus_tag="PLG78_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23434.1"
                     /translation="MEPSVDSSGQETPDSPEFMDFDLHLEYLIQQDHPRIMNLERKIP
                     QNYSWQRLLSKHFANPAFDLGRDDCEVVLEAGKKVYRIQETRKPTTFYSLLRFLEFFR
                     SNTEKLTQNLNLIEQHYDRVHKTKHENTGLRGLLRKQWTSDMIDNKHEIIAAALQKDT
                     NVDSVLGKLEHLLAENLPVTTRWQYLLQPFMNEVINHINHNLRDGAFNLRMYYPVRTM
                     EDKFKPYYSNAMRYAVELIDAEKITSAFVKRL"
     gene            3852..4148
                     /locus_tag="PLG78_09720"
     CDS             3852..4148
                     /locus_tag="PLG78_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNE23435.1"
                     /translation="MTAIELRSAPGRLEVFSPQTSEGAYRVVIIDNDVNTYEQVIQIC
                     MKALGIHYRDALEIAIAVDQNGRAEVFEGSQADAEAVASVIRTIGIEVLVVPAA"
     gene            complement(4145..5344)
                     /locus_tag="PLG78_09725"
     CDS             complement(4145..5344)
                     /locus_tag="PLG78_09725"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNE23436.1"
                     /translation="MNGNIEQAIEDIRHGRMIILVDSEARENEGDLIIAAEYADAQAV
                     NFMAVHGRGLICVPLDEETAVRLELGPMVRRNTDQFRTNFTVSVDAAHGTTTGISAAD
                     RAATIKVLADSTSRPEDLIRPGHIFPLIAAKGGSLRRAGHTEASTDLARLAGLRPVSV
                     ICEIMNEDGTMARLPDLQIFAERHGLKIYTIEDLIRYRRAHDTLIRKEVEARIPTDFG
                     EFKVNAYSTTVDDKIHLALIHGDVKGKENVPVRVHSECLTGDVFHSLRCDCGEQLARA
                     LSFISRQECGVLLYMRQEGRGIGLLNKLKAYELQDTGLDTVEANEKLGFAADLRDYGI
                     GAQMLSSLGITSMQLLTNNPRKVVGLEGHGLVITGRIPIAVEPGDENRKYLTTKKNRL
                     GHLLDTV"
     gene            complement(5341..5994)
                     /locus_tag="PLG78_09730"
     CDS             complement(5341..5994)
                     /locus_tag="PLG78_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE23437.1"
                     /translation="MASKKKSKPVTTKKAINARSKPSQAGRLSRNFARDVYVHPQATV
                     MGDVTLGQGVSIWPGAVLRGDMNSIRIGRMVNIQDNCTLHVDSSLGLEIGEYTLVGHN
                     CMIHSCTIGRGCLIGIGSVILEGAVIGDGAMITAGCLVRGGRKIPPFSLVVSKNGELV
                     VYEKKARTLLALAGCIEYSELARRAQKGKFGPFSVDEEKQFYAEAQIVYAQLFGEVK"
BASE COUNT         1503 a         1584 c         1450 g         1496 t
ORIGIN      
        1 ggcaaagaat catccggaca catacgacat catcattgtg gattcatccg atccaatcgg
       61 cccggcagaa gtgctgtttc aatttccatt ctacgaaagc tgccgaaacg cactgaagtc
      121 agatggaatc ctggcaacgc aggcagaaaa ttttctgctt catgcaaaga tcatcaaaga
      181 acttctttcg tttggtaaga aactatttcc aatttccaga tactactaca cccaggttcc
      241 cacctacccg ggcgggatga ttggatttac tttcttttct cgcaagcacg cacacgatga
      301 acttgtagac aaacgcttcg cagagtcagg gatcaaggca gaagaattcc gcttctggtc
      361 cgttgacttt cacaaaaaag cgttcactct gccgggtgct accatgcgcg atttctactc
      421 ctgattcact tcagctggtt gctgatctgg gagtgatttt gcaaagccga gtcggcaggc
      481 aggtcgatct ggaagagatt ttatatctcc gatggttgca atgttgctga gagtgatttt
      541 gcaaagtcga acccgcccgg gcggggtgtg tcccaattgc gaagccacga tggcggagcg
      601 ccgctgcgag tcgggcatgg aggctcgtct cgctgcggtt gggatacact ccggcccgcg
      661 gcgcactgag aatttcaaaa tcgcgtcatt cccactcgat cgtgccaggt ggctttgtgg
      721 tcaggtcgta aagaatggaa ccaatcccgg agattgcaag aaggcgattg gaaagatctt
      781 tcaactggtc ccgtttgatc tcgtagggag aaacagtcat cgcatccaga gacacgaccg
      841 ggcgaagaat gacagcatcc tcttccactc cctttcgcgc cagaggaaca agcacaacag
      901 gaaactgcca gatttcaccg tagatgtttt tttcgtgtag aaacgaattc acaacgtgat
      961 ctgcttcgcg cagcctttcc acccgatccg cagataattc agccttgcga accatttcag
     1021 gacgatttcc cgccacaaag cgaagaacac gattgacctc acgaacaaaa ttcggaagcg
     1081 cacgagcgat ctcatgcagg cgcgcagtgt caccttcgcg ctcgtatacg agagccgggt
     1141 gagcataggt tctctggtcc ccctggactc ccactgattt gatcggaaga atggtgcact
     1201 caatgtcctt gagcaatggc tgattgtgct cagcgagtaa agccgctgca gaacgaaagt
     1261 ctgcattgtc cgcgtcagaa cacaaaaggc gaatcgcaag cccgggccct ggaaatggat
     1321 gccgatccac gagtttctct ggcaaccgga gcaatcgacc cagctccctt acctcgtctt
     1381 tgtaaagatc gcggataggc tcaaccacct ttccttcttc tatcaatctc tgaattgccg
     1441 gaacccggtt gtgatgagtt ttgatcaaat ggcttttctt cgtgccgcca gattcaatgg
     1501 tatccgggta gatggtgccc tgcccgagca gccagtgttc cgggtccaga ttcaatcttc
     1561 gcacgcagtc tgcctggatt tccaggaata gatctccaat gattcttctt ttcttttccg
     1621 ggtccaccac accggcgaga ctggtcgcat aacgtttgcg agcgtcctct atgttcaatt
     1681 gaatcccatg cgcacgaaga gcagtaccca cttctatccc ctcatccttt cgcatgaatc
     1741 cggtatcaat aaaaagaccg tagagtcgat ccgcaggaag aatctcagat agaagagtaa
     1801 acgctaccgt tgaatccacg ccacccgaaa gtaaaaagaa aacactgcga tctccaatgc
     1861 ttcgcgccag agcttccttt tcctttgaaa gaaaatctcc aatgcgatag ctattctcga
     1921 gaccacacat gcgaataaag ttcagaagat actgatctcc atgctctgta tgctgcactt
     1981 cgggatgaaa ttgaatgcct gtaagattct tctccgcatt gaatactgcc gcattctcgc
     2041 agtcgtcggt atgtgcgatc ccaacaaatc caggtgccat gcgtgtgact tcgtctccat
     2101 gactcatcca gacagcggaa acagagttgt tttcaccgcg aagaacagac acatcgcttt
     2161 tcactttaag aatggcgcgt ccaaattcgt gatggcctga gtttcgaacc tcaccgccga
     2221 gctgctgcat catcagctgg tgaccgtaac agatgccgag aactggaagc cccgcggaca
     2281 aaagagcagg atcagaagcg ggagctcctg gctcgtacac ggaagaaggt ccgccagaat
     2341 aaatgagacc gctgtactga gcgcgcgcct cagaaggagt tagagtttcc ggatctgcaa
     2401 tctgagccag agctccgagc cttcgaatgc gcgaagcaat caaatgcgca tattgcccgc
     2461 caaaatcgac tacaccgatc ttcctgttgt ccgacattgc ggtcagaaat agtttccggt
     2521 cactgcgtgg cgaatcttaa atttcaaaaa ccagtctact ccgtttgaga cagaaacaag
     2581 cttcccatcc ttgaattgca ctagagtggg aaagacctga atccggaaag cttgctgcag
     2641 cgggccccga tccagaacga tcggaaagtt gactggatgc tcctctatga aatgagcaac
     2701 ggtgattgga tcttcgtctg taaccgcgaa gatctcaaag gattgtccat cgagttcgcg
     2761 gcgaagcgaa acgagcattg gcatttccac gcgacacggc atacaccagg ttgcccagaa
     2821 agaaacaaga atcactttct ctttacgcga agataggtca atctcagatc cagcaagtgt
     2881 gcgaagactg ccgtgaggaa gactctgccc ttcaagacct ctttcgtttt cccagagagg
     2941 gatcttgcga tacaacacaa agccaatgac aaaagcgaaa agaagaatac tactccagtt
     3001 ttttctgatg taattcagca actaaagtct cttcacaaat gcagaagtaa tcttttccgc
     3061 gtcgatgagc tccaccgcgt aacgcattgc atttgaatag taaggcttga atttatcttc
     3121 catggttcgc accggataat acatgcgaag attgaatgcg ccatcgcgaa gattgtgatt
     3181 gatatgattg atcacttcat tcatgaaagg ctgcagcaag tactgccagc gagttgtaac
     3241 tggaagattc tctgcgagca aatgctccag ttttccaaga acagaatcca catttgtatc
     3301 tttctgaagt gcagccgcta tgatttcatg tttgttgtcg atcatgtcgc tggtccattg
     3361 ctttcgcaag aggccacgaa gaccggtgtt ctcgtgcttt gttttgtgaa cccgatcgta
     3421 atgctgctca atcaaattga ggttctgggt aagcttctct gtgttcgagc gaaagaattc
     3481 aagaaagcga agcagactgt agaacgtggt aggctttcga gtttcctgaa tcctgtacac
     3541 cttcttgccc gcttccagca ctacttcgca gtcgtcgcgt ccaagatcga atgcaggatt
     3601 tgcaaaatgt ttggacagaa gcctttgcca ggaatagttc tgaggaatct tcctctccag
     3661 gttcatgatg cgaggatgat cctgctggat gagatactct agatgcaggt cgaagtccat
     3721 gaactccgga ctatcagggg tctcctggcc ggaagagtcg acggatggct ccataagatt
     3781 catgaaccag ccggtgccgg gccaatcaat taatttcttg cgcagaatcg aggatctaat
     3841 tcaaaaaaca agtgaccgca atcgagcttc gctccgcccc gggacgcctg gaggtctttt
     3901 cgcctcaaac aagcgaagga gcctatagag tcgtgatcat cgacaatgac gtaaatacgt
     3961 atgagcaggt cattcagatc tgcatgaaag cgctcggaat ccattacaga gacgcgctgg
     4021 aaatcgccat tgcggtggat cagaatggca gagcagaagt tttcgaaggg tcgcaagcgg
     4081 atgcggaagc tgtggcttct gtcattcgca ccatcggaat cgaagtactg gtcgtgcccg
     4141 ccgcctagac tgtatcgagc agatggccca gtctattctt cttggtggtt agatactttc
     4201 tgttttcatc tccaggctcc actgcgatcg ggattctacc tgtgataacc agaccatgcc
     4261 cttcaagccc gaccactttg cgcggattgt tagtcagaag ctgcatgctt gtgattccca
     4321 gagaagaaag catttgtgcg ccaattccgt agtcgcgaag gtcagcggca aaccccagct
     4381 tctcgtttgc ttccacagta tcgaggcccg tgtcctgcaa ttcgtaggct ttcagtttat
     4441 tgagaagccc gatcccccga ccttcctgtc tcatgtagag tagaacgccg cattcctgac
     4501 gtgaaataaa agacaacgct cgcgcaagtt gttcaccaca atcgcagcgg agagaatgaa
     4561 acacatctcc ggtaagacac tcgctgtgca cgcgcactgg aacattttcc ttccctttta
     4621 catctccgtg gatcaatgct aaatgaattt tatcatctac ggttgtggag tacgcgttca
     4681 ctttgaattc gccaaaatct gttggaattc ttgcttccac ttctttgcga atcagagtgt
     4741 cgtgcgcacg gcgatagcga atcaaatctt caattgtata aatcttcagg ccatggcgtt
     4801 ctgcgaaaat ctgcagatcc ggaagacgag ccatggttcc atcttcattc atgatttcgc
     4861 agatcacaga tacaggcctg agtccggcaa gccttgcaag atccgtagag gcttctgtat
     4921 gcccggctct tctcaaagac ccaccttttg ccgcaatcag tgggaaaata tgcccgggcc
     4981 gaatcagatc ctcgggacga gacgtactgt ccgcaagcac cttgatcgtg gccgcgcgat
     5041 ccgcagctga aattccagtt gtagttccgt gcgccgcatc cacgcttaca gtaaaattgg
     5101 ttctgaattg atctgtattt ctgcgaacca tgggtccgag ctctagacgc actgctgttt
     5161 cttcgtccag gggaacgcaa atcaggcctc ggccgtgaac ggccatgaag ttcacagcct
     5221 gcgcatccgc atactctgcc gcgatgatga ggtctccctc gttttcgcgt gcctccgaat
     5281 ccacaaggat gatcatgcgg ccgtgtcgaa tgtcttctat tgcctgttct atatttccgt
     5341 tcatttcact tctccgaata actgcgcgta gacgatctgt gcctctgcat aaaattgctt
     5401 ttcttcatca acggaaaaag ggccgaactt tcccttctgt gcgcgccggg ccagttcgct
     5461 atattcaatg cacccggcga gtgcaaggag agtgcgcgcc ttcttctcat atacaactag
     5521 ctctccattc tttgaaacga caagggaaaa cggagggatc ttgcgtcctc cacgcaccag
     5581 acaaccggca gtgatcatgg cgccgtctcc aatgaccgct ccttccagga tgacagatcc
     5641 gattccaatc aggcagcctc ttccgatcgt gcatgaatga atcatacaat tgtgaccgac
     5701 tagagtgtat tcgcctattt ccagaccaag cgaagagtct acatgaagag tgcagttgtc
     5761 ctgaatgttc accatcctgc caattcgtat gctgttcatg tctccgcgaa ggactgctcc
     5821 gggccagatc gacacccctt gtccaagggt gacatctccc atcacggtcg cctggggatg
     5881 aacatacacg tcccgagcaa agttccgaga aagccggcct gcctgtgaag gtttgctccg
     5941 cgcattgatt gcttttttgg tggtgaccgg cttcgatttc tttttcgagg ccagaggctt
     6001 tttcatgatc tatgataccg atggatcatt tct
//
LOCUS       DAOQJL010000192         6029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7433_length_6029_cov_4.219451, whole genome shotgun sequence.
ACCESSION   DAOQJL010000192 DAOQJL010000000
VERSION     DAOQJL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7433_length_6029_cov_4.219451"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            500..640
                     /locus_tag="PLG78_09735"
     CDS             500..640
                     /locus_tag="PLG78_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23438.1"
                     /translation="MSRERREDGLHPGDAVFTARRRKRREDGLHPGVAVSTTTPRDVN
                     TR"
     gene            760..1605
                     /locus_tag="PLG78_09740"
     CDS             760..1605
                     /locus_tag="PLG78_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23439.1"
                     /translation="MKWQKRRMQAVPLLLLRTLFVHCATEIPPANRNCNAAQATADLT
                     RLETDPSAPADNRLGDYLLAQDPTIARRARDILKKRIFIENRQYLVPPASNGPAPTRR
                     DGLFGLSHLAETNGLDSQTQVAFGHGLNDGTAENRYRTMQFLDALGRKAEPLKEQIAS
                     LFRDPDSRVANLTEFMTARFSSERFVPRQRIHEILGKSDDHVIVYGTTWCGWIQKQLA
                     GLKVGGIKHELLDAANHERNLEATVLALNYTETSTGSEDQCTGYPTIVYKYKAYVGYR
                     PDIEK"
     gene            1851..3632
                     /locus_tag="PLG78_09745"
     CDS             1851..3632
                     /locus_tag="PLG78_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23440.1"
                     /translation="MPLVHQSFEIYQDGRLIYSYGSMNGAGVIPWRSHIVPLLTWEDH
                     SEVTLRVFSRVRPVGAANIYVGSKSDVVLVMLERDLAKLLLSAIYFVVGLISLLAFFR
                     TNHQPVFLAFGIFTLLFSAYAVSNRTNEVQHLLLDAPRFWMYLEFACLYTLAPAGLWF
                     LSHVLPRVSRGLRLIIVLNLAFAAGSWFLHFTIGLDIVATVLPGLMIALATSAFGLVE
                     CIRGAIAGDRTSRLLVLAITCFAACAVFDSLVFAFAFAISKFVRWNVQTTPIGFLILC
                     GTLSYIVGNRYVEDRKKAELILIETQKNLIRSYQRFVPEQLFSLMNRDSVIDVQLGDA
                     SQREMSILFSDIRSYTTISEALGPTENFAFLNGLMRITGPVIRNHGGFIDKFIGDAIM
                     ALFPGPADDALNGAIGMKRAIEGYNAERRAQGQPPVSMGIGLNTGRVILGTLGEAERM
                     DSTVISDTVNLASRVEGLTKYYGVALLITHHTLVRLSDPTRYQYRLVDRVVVKGKTEA
                     VSIFEIFDGDADRETIVKINTQVDFERGVQEYHARNFSVAREFFQKVIRTNPNDQVAN
                     IYLSRSEHFAKHGVPVDWEGISIMEGK"
     gene            complement(3657..4127)
                     /locus_tag="PLG78_09750"
     CDS             complement(3657..4127)
                     /locus_tag="PLG78_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE23441.1"
                     /translation="MKQTTTVVNEIDISISPEMVFDLVSSPGLWPRWHPSSRHLAPGA
                     DRPLKKGETFSEEIKAGGREGKLQWIVTECDRPRLWVATASSDQGVTIQLKYETEKNS
                     AGARFKRTLNYTMTSPFLILANTIFMRFKIRRESAHALGNLKQLLEKGVSSDVV"
     gene            complement(4442..4786)
                     /locus_tag="PLG78_09755"
     CDS             complement(4442..4786)
                     /locus_tag="PLG78_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE23442.1"
                     /translation="MGLLFIWGSIAFFFHMYPKPELTGNLKIFNEGIDASGYLMVLVK
                     SVELICGIAFLSGRFVTLASVVIAPIIVNILCVHIFLDHTGLPVAIFLVLANGFLAYL
                     NRENYKSLFVVK"
     gene            5067..5612
                     /locus_tag="PLG78_09760"
     CDS             5067..5612
                     /locus_tag="PLG78_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23443.1"
                     /translation="MTSRAAAVFPLFFFLLKVYQYRMPADQAQILWWCTISNLGLGLS
                     MLLGFARGVWIFSFMLILGTPIWLLDVWATGDFTIYSVFTHVISPALGIYSVRKLSVP
                     RGIWWQGMIYYFSLILIARALTPPALNVNLAHDVYAAAKPFIQNYWLYTAMNSILLAT
                     SLYVLGRLASQRFPLARGPRT"
     gene            complement(5648..>6029)
                     /locus_tag="PLG78_09765"
     CDS             complement(5648..>6029)
                     /locus_tag="PLG78_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase"
                     /protein_id="HNE23444.1"
                     /translation="ERMEVTVISDAVNQASRMEGLTKAYGVGTIISETTFRELANPQD
                     YSLRLLGSFSMKGKSNNVTLLEILDGLPAAEFELRKSTRSDFEHGVFSHLNGQLEEAR
                     SYFGKVLAVNPLDEAAKYYVSICK"
BASE COUNT         1435 a         1607 c         1496 g         1491 t
ORIGIN      
        1 acgccaggtt caggtcctgg tgggagcaat cctgtggagg ttcaagtcct ctcctcggca
       61 ttttcacgac gccgtttccc tcacccgcta gtcgacacgc gcacttccgc tttcggcaca
      121 ggactagaac caccgtccgc ttcgcgttcg gcacgtcgct ccccatgggt cgctccgtgg
      181 gttcaagtct cactgtaccc agcactacaa cgcccgcaca aaacccgaaa gtctctcagg
      241 ctgtgcagta tgctgcagcg cccgggtgca atccacagcg gcgttcggag cgtccgagcg
      301 tcgcataaat gtgtgtccgt gggttcaagt cttcactgtc cctggcaatg catctcccgc
      361 acacaacctg aaagtctcta agactttgca gttagctgca gcgcccgggg tgcaagccac
      421 agcggcgttc ggagcgtccg agcgtcgcaa cggcgtcgcg aggatggctt gcatcccggg
      481 cgttgcagtt tcttactaaa tgagtcgcga gagacgtgag gatggactgc atccgggcga
      541 tgcagttttc actgcacgac gtcgcaagag acgtgaggat ggactgcatc cgggcgttgc
      601 agtttctact accactccca gggacgtaaa cactcgctga ataattgact gatcagtatt
      661 ctcttaggaa ccactctgct cttatgtatg caaaattgcc tattccttcc agaacctaaa
      721 acgcattgac ctttggcagc aaggctccag actccattca tgaaatggca aaagcggcgg
      781 atgcaggcag tcccacttct gcttctccgc accctctttg tccattgcgc aacagaaatc
      841 ccgccggcaa atcgtaactg caacgccgcg caagccacgg ccgatctgac gcggctcgag
      901 acggatccgt ccgctcccgc ggacaatcga ctgggtgact acttgctcgc gcaagatccg
      961 acgatcgctc gccgtgctcg tgatatttta aaaaagcgaa tatttatcga aaacagacag
     1021 tatcttgtcc cacccgcgtc caacggtccg gcgcctacgc gccgtgacgg tctctttggc
     1081 ctgagccatc ttgcggaaac gaacggactg gattcgcaga cgcaggtcgc tttcgggcat
     1141 ggcctgaatg atggcacggc ggaaaacaga taccgcacca tgcagtttct agatgcgctc
     1201 ggaagaaaag cagaacccct caaagaacaa attgcatcac tttttcgaga cccggattcg
     1261 cgtgtagcaa acctgaccga atttatgact gcgcgattca gttcagagag attcgtcccg
     1321 cgccagcgaa ttcatgaaat ccttggcaaa tcggatgacc acgtaatcgt ctacggcacg
     1381 acgtggtgcg ggtggattca aaagcaactc gctggattga aagtgggagg catcaaacat
     1441 gagcttctgg atgcggcaaa tcacgagcgc aatctggaag cgaccgtgct tgctttgaac
     1501 tacaccgaga cgagcaccgg gagtgaggat cagtgcacag gatatccaac aatcgtatat
     1561 aaatataaag cttatgttgg ctatcgccct gatatcgaga aatgatgttg cgtgctgtgg
     1621 ctttcagcgt tctggtgatt agcgccgtca atgctttcgg atgcacgcac caccagacga
     1681 ttatcgatct gacttcgaaa gttgaggtat taacgaatga acaacctgaa ccattctgga
     1741 cgcaattgtc caaacacgac aatgcattcc tggttgagaa gccggaaccg cgtattcgaa
     1801 tatttcgggt cgcattgcca aaaagcgttc cgcccgaacc ggttttgcac atgccgctgg
     1861 tccatcaaag cttcgagatc tatcaggacg gaagattaat ttactcttac ggttcgatga
     1921 atggcgctgg agtaattccg tggcgttcgc acatcgtacc cctcctcaca tgggaagatc
     1981 acagcgaagt cacgttaagg gtcttctcga gagtgcgacc cgttggcgcc gcgaatatat
     2041 acgttgggag caaatcagat gttgtcctcg ttatgcttga gcgcgatctg gcaaagcttt
     2101 tgctgtccgc tatttacttt gttgtggggc tgatttccct gctggctttc tttcgcacga
     2161 accaccaacc agtattcctt gcgtttggca tttttacgct gctcttcagt gcctatgcag
     2221 tctcaaaccg cacaaacgaa gtccaacatc ttctcctgga tgcccctcgg ttctggatgt
     2281 atttggaatt tgcatgtctg tacacgctgg cgcccgcggg cctgtggttc ctgagtcatg
     2341 ttctcccgcg cgtcagccgg gggcttcgcc tgatcattgt tttgaacctc gcatttgccg
     2401 caggatcctg gttcctccat ttcaccattg gcctcgatat cgttgcgact gtcctgcccg
     2461 gattgatgat tgcgctggca acgtccgcct ttggcctcgt agaatgcatt cgcggagcga
     2521 tagccggcga ccgcacgagt cgtcttctcg tgctcgcgat cacgtgtttc gccgcatgcg
     2581 cagttttcga ttctctcgtg tttgcttttg cgttcgctat cagtaagttt gtgcgctgga
     2641 acgtacaaac cacccccatt ggctttctga tcctttgtgg aactctgagc tatatagtcg
     2701 ggaaccgcta tgtggaagat cgcaagaagg cggaactcat attgattgaa acgcaaaaga
     2761 acctcattcg gtcctatcaa cgcttcgtac ccgaacaact attctcgctc atgaaccgcg
     2821 acagcgtaat cgatgtccag ctgggggacg cttcacagcg cgaaatgagt attctctttt
     2881 ctgatatcag atcctacaca actatctcag aagcactcgg gccaaccgaa aactttgcgt
     2941 tcttgaacgg tttgatgcgc attacaggtc ccgtgatacg caatcacggc ggtttcattg
     3001 ataagttcat cggagatgca atcatggcgc tttttcctgg accggcggac gacgcattga
     3061 acggcgcgat cggcatgaag cgcgcaattg aaggttataa cgcggaacgc agggctcagg
     3121 gacagccccc tgtctccatg ggaattggcc tcaacaccgg cagagtaatt ctcggaaccc
     3181 tgggtgaagc cgagagaatg gacagtactg ttatcagcga tacggtaaac ctggcttcgc
     3241 gggtcgaagg actgacgaaa tactatggag ttgcgctttt gatcacgcat cacaccctcg
     3301 tgcggttaag cgatccaaca cgctatcaat accggctggt cgatcgtgtc gtcgttaaag
     3361 gaaaaacaga agctgtttcg atttttgaaa tcttcgacgg ggatgcagac agagagacaa
     3421 ttgtcaagat caatacccag gtcgatttcg aaagaggtgt tcaggaatac cacgccagga
     3481 acttctcagt ggcccgggaa ttttttcaaa aagtaatccg gacaaatccg aatgaccagg
     3541 ttgccaacat ctacctcagc aggtctgaac attttgcgaa gcatggcgta cccgtagact
     3601 gggaaggaat ctcgattatg gagggaaagt gaatgcagtg gcattgcgca tttccactac
     3661 acgacgtcgc tagagacgcc tttctcaaga agctgtttca gattgcccag cgcatgcgcg
     3721 gattcgcgtc tgattttgaa ccgcatgaag atggtgttgg ccaatatcag aaatggactg
     3781 gtcatggtgt aattcagagt ccttttgaag cgcgcgccgg ccgagttctt ctccgtttca
     3841 tatttcaatt gaatcgtgac gccctggtca ctgcttgctg tggcaaccca tagacgaggt
     3901 ctgtcgcatt ctgttacgat ccactggagt ttcccctctc tgccgcctgc tttgatttct
     3961 tctgaaaacg tttccccttt tttcaaagga cgatccgctc caggagcaag gtggcgagaa
     4021 gacggatgcc atctgggcca taggccgggc gagctcacca gatcaaagac catctccggc
     4081 gaaatggaaa tatcaatttc atttactacc gtagtggtct gtttcattta taagttcttc
     4141 taccgaggtt gcaggaaagg caagcagtat tgaaatgtcg gcaaccaatc aagtccattg
     4201 aattacaaaa tatatcatct cccgcacaca acctgaaaag tctctcagac tcatgcagta
     4261 cctgcagcgc ccgggtgcag tccacagcgg agttcggagc gtccgagcgt cgcttcagcg
     4321 cgtcgcgagg atggactgca tccgggcgtt gcagtttcta acacatgagt cgcaagagac
     4381 gccgaggatg gtttgcatcc cgggcgctgc agtttcttac tacatgagtt gcaagagacg
     4441 cctacttcac gacaaagaga gatttatagt tctctctgtt cagatatgca aggaagccgt
     4501 ttgcaagaac gagaaaaatc gcaactggaa gcccggtgtg atctagaaag atgtgcacgc
     4561 acaaaatatt cacgatgatt ggtgcgatca caacgcttgc cagagtcaca aatcgacctg
     4621 aaagaaacgc aatcccgcaa atcaactcaa cggatttgac aagaaccatc aaatatccgg
     4681 atgcatcgat tccttcgttg aagattttca agttacccgt gagttcgggc tttggataca
     4741 tgtggaaaaa gaacgcgatc gacccccaga taaaaagtaa tcccatcagc gtacggacaa
     4801 ctatgccagc aattttcatt tcgaaatcct ctacggcaac catttgaaat ggagggcgtg
     4861 cttacaattg ttttgcgaga cgattgcatg ttttttccgc gtgattcatg actcgatttt
     4921 tcgtgcccgg cggccgccgc ataacacgcg ttagcttaca atctccttct cgatcactca
     4981 aggggtgaaa ccacaagcaa cgagaacgcc ctggaggact ttttttgatt gcacgcaagg
     5041 cgcaaacgca tggccaatga aacacgatga catctcgtgc agccgccgtt ttccccctgt
     5101 tcttctttct gctgaaggtt tatcagtaca ggatgcctgc agatcaggca caaattctat
     5161 ggtggtgcac aatttccaat ctcggactcg ggctatccat gcttctcggt ttcgcgcgag
     5221 gcgtctggat tttttcattc atgttgatac tgggaactcc catctggctt ctagatgtat
     5281 gggccacagg cgatttcacc atctattccg tattcacgca tgtgatcagc cccgctcttg
     5341 gaatttatag cgtaaggaaa ttgtcggtac cgcgcggaat ctggtggcag ggaatgatct
     5401 actacttctc tctcattctc atcgcgcgag cccttacacc gccggctctg aacgtaaacc
     5461 tcgcacacga cgtttacgcc gcggcaaaac ctttcattca gaattactgg ctgtatacgg
     5521 cgatgaattc tattctgctt gctacgtcac tttatgttct gggaaggtta gccagccaga
     5581 ggtttcctct agcgcgaggc cctcgcacat aacgttaggc gactcactct gacttttcac
     5641 cctgacttta tttacaaata cttacgtaat atttcgctgc ctcatccagg ggattgaccg
     5701 cgaggacttt gccaaaataa gagcgagcct cttccaactg cccgttcaaa tgactgaaga
     5761 caccgtgctc aaaatctgat cgcgttgact ttcgcaattc aaactccgcg gctggaagcc
     5821 catcgaggat ttcaagcaag gtaacgttgt tgctcttgcc tttcatggaa aaagaaccaa
     5881 gcaatcggag ggaatagtcc tgaggatttg caagttcgcg gaatgttgtt tcactgatga
     5941 tggttccaac gccgtacgcc tttgtgagtc cctccatacg cgacgcctgg ttcacagcgt
     6001 ccgaaatcac cgtcacttcc atcctctca
//
LOCUS       DAOQJL010000193         6004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7488_length_6004_cov_4.149941, whole genome shotgun sequence.
ACCESSION   DAOQJL010000193 DAOQJL010000000
VERSION     DAOQJL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7488_length_6004_cov_4.149941"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="PLG78_09770"
     CDS             <1..2632
                     /locus_tag="PLG78_09770"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23445.1"
                     /translation="VGSFFAETSLTIIPGLATLGLALYYFERLFPQDKSKVLRRFDYI
                     SLAVFGTSAIAFVITYASSASSVFHRVKSILPEPLSALGPDLYTFCLWFSFFWLIIGA
                     IYTFQGLARAGVDRGNLGGLIIAIMTSFFFALSLAKFPLFKSLENTALVNRYAFLRSP
                     SGTLAPGGAAAYCQAPYDDKGEKVVAMAPPPPGVRDDIRIVGISNETIEKVNFKWPLD
                     WNIYADLASTLGGAENGIILFDISFVDEKGLYGGDACGVILDCRPLSADRPPRRQVDI
                     LAESFSKSKAAIISDYPLETSDQFLKPIRQNPVSSDRYNRRVEMMYAQNRLTNVKNGQ
                     LAEPGLSFPNPAVEPIGKAQKGAGYANILKNEESGMNWQMPLVQRVVNLDKFRAPGYD
                     SNRDDIFLPGIDLIVAALYYGIDLKKDVAVDYAAGTITLSNIPEKYKEKVNLATFENE
                     KLDIMARPNASRTIVIPIDRRGQMHISFRGGQYCFPFQEILEVNKMTPAEGAANFKDK
                     IVLVAMYYATGVGTAQDIHLSPYGDMAGIEHHAYAINTILNQDFIYEVPPVVNLLILL
                     AVGLIMGFYQPRVATWLAFVFAIVIAAVYSVMTLFITFQVFNVLHVFPTVIILQFVQL
                     VGFIGFRVLTEEENVKFIRNTFSKFVSQDVVEELLSNPEAIALGGSKKEISVFFSDVR
                     GFTTISEALGPEELVNLLNEYLSEMTELIIEYRGTIDKYMGDAIMAFWGAPAKNDDHA
                     YYACVASLAQYHALQSLQKRWSERNIPVLDIGIGINTGMAVVGNMGSSRRMDYTLMGD
                     TVNLGSRLEGATKNYGVKIIISEFTYERVKDRVYARELDLVRVKGKLEPVRIYELMAL
                     KNDADVEALKTSNAHKDTAA"
     gene            2632..4545
                     /gene="dxs"
                     /locus_tag="PLG78_09775"
     CDS             2632..4545
                     /gene="dxs"
                     /locus_tag="PLG78_09775"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNE23446.1"
                     /translation="MDLLSKINSPADLRKVAPEDLPRVCDEVREYLIDTLASVGGHFA
                     SNLGVVELTVALHYVLNTPEDKLIWDVGHQIYPHKILTGRREQLRSVRRRGGLSGFPK
                     REESVYDLYNTGHAGTSISQALGEAMARDRLGLNHQCVCVIGDASVASGMAFEALNHG
                     GHVQTDCIVILNDNDMSISKNVGALNQYLNRLITSPTYNKGKRIWYSILMWLPLIGPL
                     VQIFSRKIERTFKDFLTPGSLFSDFGFRYIGPVDGHNVAELVEVLEKAKELKGPVLIH
                     AYTQKGKGYVHAERDPIVYHSVSRFNREDGTMQGSKARGKISFSEIVGETLCEIIEHN
                     EKVVVITPAMIEGSGLRSVADRFPNRVFDVGIAEQHSMAFAGGLASGGAVPYLCIYST
                     FMSRAYDQLIEDVALMNFPVKLIVDRAGCVGPDGETHQGLYDLGVLLSVPNVRVYAPA
                     TGGELKAFLKFVEKDSQGPIAVRFPKDSADRESLLPANLPDVTRIRPEIHQGGKDLCI
                     LAVGIMWETALGVQKELRAHGIYATVLGIRWIRPLDIEFIQNTIHDCEYMVILEDSAI
                     NSSAASHIRWSLNSASNAKHLHTFAFPVEAIVHGTREEIMHDHGLSKEAITGALLARP
                     EFANRRIPLARQA"
     gene            4545..>6004
                     /locus_tag="PLG78_09780"
     CDS             4545..>6004
                     /locus_tag="PLG78_09780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNE23447.1"
                     /translation="MFSGWENLGEYLDSYTYRQSQEKFSDSIQRALADQAVLIGEAGT
                     GTGKTIAYLIPLLLYAVETETRVAVSTDTKSLQTQLLTKDIPLVESILGLSPGAELCL
                     GASNYICKRKMKTAVDQGGLLLAGSELDSLIAWEEETLSGIRSDYPGSVGESVWGAVT
                     RETDNCLGRRCPNYQESYYFVAREKWKKARILVVNHALLASHMALEGKLLPEFSAVVV
                     DEAHKFPDAALDAMKQRLILGELSHLIRSSKRRADRILQTIESYRQEVEGKYRNAQRI
                     RTPAGTSSGHRLIEQIERIEEELREEMQEKENPDNEGDLKAQMLLGRLGDARRVMERF
                     EEGPDTDHVHWVTVEGQKGVIMHRSPLDAQSFIRSELLEKHSSVIFTSATLGASGKKP
                     FSYFARETGCDRAEKEVRYARVGSPFDYEKRTLLFLPPDIVEPSDEDRFPQDCAYWID
                     RMVGLTGGGAFILFTSRLMLSRVHALLEAKESGISY"
BASE COUNT         1523 a         1437 c         1554 g         1490 t
ORIGIN      
        1 ggtaggttcc ttcttcgcgg aaacatccct gaccatcatt ccaggactgg ccacacttgg
       61 attggctctc tactattttg agcgattgtt tcctcaggat aaatccaaag tactgagaag
      121 attcgattac atctcacttg ctgtattcgg aacgtccgca atcgcgttcg tcatcactta
      181 cgcaagcagc gcttcctctg tttttcatag agtaaaatcc attctacctg aaccactctc
      241 tgcactcggt ccggatcttt atactttctg tctctggttc agtttcttct ggctgatcat
      301 tggtgctatt tacactttcc agggtctcgc tcgcgcaggt gtggatcgcg gaaacctggg
      361 cggtttgatc atcgccatca tgacttcgtt tttctttgca ttgtctcttg cgaagtttcc
      421 gcttttcaaa agtcttgaaa atacagcact tgtaaaccgt tatgcgttct tgcgcagtcc
      481 aagtggcact cttgctcctg gaggagcggc cgcctactgt caggcacctt acgatgacaa
      541 aggcgaaaag gtagtggcca tggctccacc tccgccaggc gttcgcgacg acattcgaat
      601 tgttggtatt tctaacgaaa cgattgaaaa agtgaatttc aaatggcctc tggactggaa
      661 catttacgca gaccttgctt ccactctcgg aggcgcagaa aacggaatca tcctgtttga
      721 cattagcttc gtagacgaaa aaggactcta cggcggtgac gcctgtggtg tgattctgga
      781 ttgtagaccg ctgagtgcag atagacctcc gcgtagacag gtagacatcc ttgcggaaag
      841 tttttcaaaa tcaaaagccg ctatcataag tgactaccct cttgaaacgt ccgaccagtt
      901 cctgaaacca attcgtcaga accccgtttc cagtgaccga tacaatcgtc gcgtggaaat
      961 gatgtacgcc cagaatagac tgaccaacgt aaaaaatggg cagcttgcag agcccggtct
     1021 ttcctttcca aaccccgcgg tcgaaccaat cggcaaagca cagaaaggcg cgggctacgc
     1081 gaacattttg aagaacgaag aatccggtat gaactggcag atgcctctgg ttcagagagt
     1141 ggtgaatctg gataagtttc gcgcgccggg ctacgactcc aatcgcgacg acatcttcct
     1201 tcctggcatt gacttgattg tcgcggctct ttattatggg attgatttga agaaagatgt
     1261 ggcagtagac tatgcagctg gcaccatcac tctaagcaat attccagaga aatacaaaga
     1321 gaaagtaaat ctggcaacgt ttgaaaacga gaagctggac atcatggcgc gcccgaatgc
     1381 atcgcgcacg attgtaattc caatcgacag acgcggacag atgcacatca gtttccgcgg
     1441 cggacagtac tgctttcctt tccaggaaat tcttgaagta aacaagatga ctccggcaga
     1501 gggagccgcg aacttcaaag acaagatcgt acttgtggcc atgtactacg ccaccggtgt
     1561 aggaacagcg caggatattc acctttcccc ttacggtgac atggcgggca ttgagcacca
     1621 cgcatacgcc atcaacacga ttctaaacca ggatttcatc tacgaagttc cgcctgtcgt
     1681 gaatcttctg attcttcttg cggtaggctt gatcatgggc ttttaccagc ctcgagtggc
     1741 cacatggctc gcgtttgttt ttgccatcgt aattgcggct gtttattcgg taatgactct
     1801 gtttatcacc ttccaggtgt tcaacgttct gcacgtgttt ccgaccgtga tcattttgca
     1861 gtttgtgcaa ctggttggtt tcattggatt tagggtgctg acggaagaag aaaacgtgaa
     1921 attcattcga aacacattct ccaagttcgt atcgcaggat gttgtggaag aacttctctc
     1981 gaaccctgag gcgatcgcac tgggtggatc caagaaagaa atcagcgtgt ttttctcaga
     2041 cgttcgcggg tttacaacga tctcagaagc acttggtcca gaagaactcg tgaatctgct
     2101 caatgaatac ttgagcgaaa tgacagagct catcattgaa tatcgcggca ccattgataa
     2161 gtacatggga gatgcgatca tggcattctg gggcgctcct gcgaaaaatg acgatcatgc
     2221 ctattacgca tgcgttgcat ccctggcgca gtatcatgct ctccaatctc tgcagaagag
     2281 atggtcggag cgaaacattc cggttctgga cattggaatt ggaatcaaca caggtatggc
     2341 ggtcgtaggg aacatgggct cgagccgtcg tatggactac actctaatgg gagacactgt
     2401 gaacctggga tcgcgccttg agggtgcgac caagaactat ggtgtaaaaa tcattatttc
     2461 tgaattcaca tacgaacgag tgaaggatcg cgtatatgcg cgtgagctgg atcttgtgcg
     2521 ggtgaaggga aagctggaac ctgttcgcat ctatgaactg atggcactca agaacgatgc
     2581 agatgtagag gctctgaaga cttcgaacgc acataaggac acagccgcct gatggatctt
     2641 ctttcgaaga tcaatagtcc ggcggatctg agaaaggtcg cgccggagga cttgccccgt
     2701 gtttgcgatg aagtgcgaga gtatctcatc gatactcttg cgtccgttgg tgggcatttt
     2761 gcttccaatc tgggagtggt ggaactgacg gttgccctgc attacgttct caatacccct
     2821 gaagacaaac tgatctggga tgttgggcat cagatttatc cgcataagat actaaccgga
     2881 aggcgagaac aacttcgcag cgtgcgccgt cgcggaggtc tatctggttt tcccaaaaga
     2941 gaagaatccg tctacgattt gtacaacaca ggacatgcgg gtacttccat ttcgcaggca
     3001 ctcggagagg ccatggcccg cgatagactg gggctgaatc atcagtgtgt gtgcgtgatt
     3061 ggagatgcat cggtggccag cggcatggca ttcgaggcgc tcaaccatgg cggacatgtg
     3121 cagacggact gcattgtcat tctcaatgac aatgacatgt ctatatccaa gaatgtgggc
     3181 gcactcaacc agtatctcaa tcgtctcata acatctccca cttacaacaa gggaaagcgc
     3241 atctggtatt ctatcctgat gtggctgcct ttgatcggac cgctcgttca aatcttttcg
     3301 cgcaagatag aaagaacgtt caaagacttc ttaactccag gcagcctatt ttctgatttt
     3361 ggatttcgct acattggacc tgtggacgga cacaatgttg cagaacttgt agaagtgctc
     3421 gagaaagcaa aggagctcaa gggccctgtt ctaattcacg cctatactca aaaaggaaag
     3481 ggatacgttc acgcggagag agatccaatc gtatatcata gcgtatctag attcaatcgc
     3541 gaagacggaa ccatgcaggg ttcgaaagcc cgtggtaaaa tttcattttc cgagatcgtt
     3601 ggtgagacgt tatgcgaaat cattgaacac aatgagaaag tagtggtgat cactccagcc
     3661 atgattgaag gctccggcct cagatctgtt gcggatcgtt tcccgaatcg cgtatttgat
     3721 gtgggaattg cagagcaaca ttccatggct tttgccggag ggctcgcctc gggtggagcc
     3781 gttccctatc tatgcattta ttctacattc atgagtcgcg cctatgatca gttgattgag
     3841 gatgtggctc tcatgaattt cccggtgaag ttgattgtgg atagagcggg ttgtgttgga
     3901 ccggacggag aaacgcacca gggtctgtat gatcttggag tgcttctcag tgtgccaaat
     3961 gtgcgtgtgt atgcgccggc tacgggcgga gaactcaaag cattcttgaa atttgtagaa
     4021 aaagattccc agggacccat cgccgttcga ttccccaagg actcagcaga tcgggagtct
     4081 ttgcttcctg ccaatctccc ggacgtgact cgaattcgcc ctgaaatcca tcagggagga
     4141 aaggatcttt gtattctggc agttggcatt atgtgggaga cagctcttgg agttcagaag
     4201 gaactgagag ctcacggaat ctatgcgact gtgctaggca ttcgctggat tcgtcctctg
     4261 gacatagagt tcattcaaaa tacaattcac gattgcgaat acatggtcat cctggaagac
     4321 tctgccatca acagcagcgc tgcatctcac attaggtgga gtctgaattc tgcgagcaat
     4381 gctaagcatt tgcatacttt tgcatttcca gtggaagcaa ttgttcacgg cactcgtgaa
     4441 gagatcatgc acgatcacgg cctgagcaaa gaagcgatca caggtgcctt gcttgcgcgt
     4501 cccgaattcg caaatcgcag gatacctcta gcaaggcagg cctgatgttc agtggctggg
     4561 agaatctcgg cgaatacctg gacagctaca cctatcgcca gtctcaggaa aagttttcag
     4621 actctatcca gcgtgccctc gcagatcaag cggtcttgat cggcgaagca gggaccggca
     4681 caggcaagac gatcgcctat ctcattcctc ttttgctcta tgctgtggaa acggaaactc
     4741 gtgttgccgt atccacggac acaaaatcac tccagacaca gctcctaaca aaggacatac
     4801 ctcttgtaga aagcatactt ggcctatctc ccggagctga gctttgcctt ggcgcgtcca
     4861 actatatctg caaacgaaaa atgaagactg cagtggacca gggcggtctg cttcttgcgg
     4921 gctctgagct tgattctttg attgcatggg aagaagagac gttaagcggc attcggtccg
     4981 actatcctgg cagcgtggga gagtctgtat ggggagcagt cacgcgcgag acggacaatt
     5041 gtcttgggag aaggtgcccg aactatcagg agtcctatta ctttgttgcc agggagaagt
     5101 ggaagaaagc ccgcatcctt gtggtcaatc acgctctcct tgcatcgcat atggccctcg
     5161 agggaaagct actccccgag ttcagtgcgg tggtcgtaga cgaagcgcac aaattcccgg
     5221 acgcggccct ggacgcgatg aagcaaagac tcatactcgg agaactttcg catctcattc
     5281 gatcaagcaa gagaagggcg gatagaattc tacaaacgat cgaatcgtat cgtcaggaag
     5341 tagaagggaa atacagaaac gcacagagaa ttcgaacccc cgcgggcact tcgagcggac
     5401 acagattgat tgaacaaatc gaaagaattg aagaagagct cagagaggag atgcaagaaa
     5461 aggaaaaccc ggacaacgaa ggagatctca aggcgcagat gctgctcggg agacttggag
     5521 atgcgcggcg ggtcatggag agatttgaag aaggtccgga cacggatcac gttcactggg
     5581 taaccgtaga gggacaaaag ggtgtaatca tgcaccgcag tcctctcgat gctcagtctt
     5641 tcattcgctc tgagctttta gaaaagcatt cgtctgttat ttttacttcc gcaactctcg
     5701 gagcatctgg caagaagccc ttttcatatt tcgcacgcga aacaggctgt gatcgcgctg
     5761 agaaagaagt gcggtacgcg cgcgtgggtt caccttttga ttacgaaaag cgaactctcc
     5821 tctttcttcc gccggacata gtggaacctt ctgacgaaga tcgattcccg caggattgcg
     5881 cctactggat cgacaggatg gtagggctca ccggcggcgg agcattcatt ctgtttacat
     5941 ctcgtcttat gctttcgcgc gttcatgctc tcctcgaagc gaaagaaagt ggaatctcct
     6001 acat
//
LOCUS       DAOQJL010000194         5971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7550_length_5971_cov_4.754226, whole genome shotgun sequence.
ACCESSION   DAOQJL010000194 DAOQJL010000000
VERSION     DAOQJL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7550_length_5971_cov_4.754226"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2833
                     /locus_tag="PLG78_09785"
     CDS             <1..2833
                     /locus_tag="PLG78_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23448.1"
                     /translation="LFYHFPRTRYPKVARTVLTIQVSIGVILTAAFIYTSLSRGAVYS
                     FSGHFWDFDMDDWSKAVALVALLNLVHLIVAAVVHIIVVPEKRLALGLMFFGFMFAIL
                     IPTVTNIMSRDGLFPRDLHQTLLVLLSITGLFTVLIVYLNHTQDKTTFMVKIVGISLV
                     TFMVVMVFITYFSFQDKEDAYDAVRIERTSRLAIEKNYPVDDLAYLVSYDAVSGKIEV
                     LSGKPDAIDYSDTEPEFASAVIYERIRNLNQTEWKSGLEEILKNSPEEFQGYAGFIRS
                     LAVTAPSAQAVLALVDKDSQQFTYRSIKIRELPDKDFRKLLTAYNDKLPPKLASFAEA
                     IRRYLASHPDMDGAKLKQEVLHFFSPFHPETTRRYRSDAANHHYTAFMKFDHQHKILY
                     EAGYRYIDYRRFIQPVGVRLTLVFITVVLTIIFGFRLFFLGTLVRPLQALLSGVRKVN
                     KGNLAVHVPVGVADEIGFLTESFNGMVSSIRIAREKLERHADELEVKVRERTAELTET
                     LQTVQDLKSQQDGDYFLTSLLIKPLARNTARSETVHVEFLMEQKKKFEFRKWKEEIGG
                     DFCSAHTVELAGKRYTVLVNADAMGKSIQGAGGALVLGAVFESIMERTRSIPSFRDQS
                     PERWLKNAFQELHGIFESFDGSMLVSMVLGLLDDESGLLYYVNVEHPWTVLYRGGKAS
                     FIETEHQFRKLGVAGVEGQIRVQTFQMEPDDVLILGSDGRDDILLGTSESGERIINED
                     EHLFLKMVEAAQGRLPEIRSHLEEQGPLTDDLSLIRVAYRSHEEDQAPDREDAMTAYR
                     EKARQLMEENKFAEAAVALGDALQLDPRSLKTIRMMVRLFLDQKDYDRAFAQVEDYIY
                     LRPLDTDMIYLASYLCKKLKKLRDAAEFGERVRLRAPDMVRNLLNLSEVYARLGNKDR
                     SAKILREVYKLEPDNKKADKLRKLLETAA"
     gene            complement(2841..3623)
                     /locus_tag="PLG78_09790"
     CDS             complement(2841..3623)
                     /locus_tag="PLG78_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE23449.1"
                     /translation="MIKTPNVFNLIYSVVLWAILLTVTLFFGISVMPFALFRARKAVH
                     FGARMWGKSLAFLSRMTIQVKNKEKLYRGGPVIYLANHQSMFDIAVFYSFLDTEFRWM
                     AKASLFKIPFVGWGMTGAGYIPVERGDRKKAMESLFQAADRIQGGASVIIFPEGTRSE
                     NPHEGMLPFKKGAFVLAKKANVTIQPITIWGAHHIMPAKQKVWVQRIYPGLVQVEVHD
                     PISPEESGKMDQEQLSAHVRTVIERPMKVMLETLDDNEKKRG"
     gene            complement(3660..4673)
                     /locus_tag="PLG78_09795"
     CDS             complement(3660..4673)
                     /locus_tag="PLG78_09795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNE23450.1"
                     /translation="MEGESFYSLTPESILASVEAAGLPVTGRCLQRNSLENRVYEVEI
                     DIESENKSERFRIAKFYRPGRWTREQILEEHQFISDLRAQEIPCAPPIALPGGSTLGK
                     TESGIFFSLFERVGGRQLDELGGTEREQLGRLLARVHNVGAASKSVHRIKLNEETYGL
                     QNLDYLLESESIPEHMEERYAQLVEDICEHTANLREELSAPNEFLPALEEIRLHGDCH
                     GGNILWSNAGPFLIDFDDMVSGPPVQDLWLLASDSEELEAIVNGYEQMRTFDRRSLKL
                     VEHLRALRYIHFSAWIARRWEDPSFPGAFPAFGTEQYWATQIDDLVEQLERIETTQSP
                     FSD"
     gene            4754..5440
                     /locus_tag="PLG78_09800"
     CDS             4754..5440
                     /locus_tag="PLG78_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE23451.1"
                     /translation="MNEPSQLLYFGDPMCSWCYGFAPVLLRLKEDLEGRLPIHCVLGG
                     LRPGPHAEKMDEKLKRFLRHHWEEVHRTTGQEFGYKIFDRDDFVYDTEPSCRAVAIVR
                     KLKPDAVIDFFAQVQTAFYRDSKDTTMLETYIPLAAEIGVSPEEFTALWNSEDMKAET
                     WKDFEFAASAGVRGFPTLIYIGEGRGQIICRGYLPYEDVRERVETVLTARKPVLAPTQ
                     EACDIEKGIC"
     gene            complement(5448..>5971)
                     /locus_tag="PLG78_09805"
     CDS             complement(5448..>5971)
                     /locus_tag="PLG78_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HNE23452.1"
                     /translation="HPKAEKFYCIRAILPIFEPVKVGIQTGSLKGRAAGSARTSTGES
                     TTAEKNSFLDLLTEVLPASTPERASLQNLWSQMPQAERDLIDNPSKDNLAAYQDLVRA
                     IARQTLDEAMKVEKRTRPATFSRAQNELTTVRIIDEKLHRMALVMLSPENSAFSMMKS
                     LAEIRGFLMDMRR"
BASE COUNT         1414 a         1410 c         1556 g         1591 t
ORIGIN      
        1 gctcttctat cacttcccac gcactcgtta tccaaaggtg gcgcgcacag tgctgacgat
       61 ccaggtgtcg attggagtga tcctgactgc agcgtttatc tatacatccc tttcgcgcgg
      121 agcggtgtat tcattctccg ggcacttctg ggattttgac atggacgact ggagcaaagc
      181 ggttgcgctc gttgcgctct tgaacctggt tcatctgatt gttgcggctg tcgtgcacat
      241 catcgtggtt ccagaaaaac gtctcgcgct tggattgatg ttcttcggtt tcatgtttgc
      301 gattttgatt cctactgtca caaatatcat gagtcgcgat ggtttgtttc cgcgcgatct
      361 gcatcagact cttctcgttc ttttgtcgat tactggtttg tttacagtgc tgattgtcta
      421 tttgaatcac acccaggata agactacatt catggtgaag atcgttggaa tcagcctggt
      481 cacattcatg gttgtaatgg ttttcattac ttacttttca ttccaggaca aggaagatgc
      541 gtacgacgcc gttcgcattg aaagaacgtc gcgccttgcg attgagaaga attatccagt
      601 cgatgatcta gcgtatcttg tttcgtatga tgcggttagc ggaaaaattg aagtcctttc
      661 cgggaagccg gacgcaatcg actattcgga cacggagccg gaatttgcaa gtgcagtgat
      721 ttacgaacgc attcgcaacc tcaatcaaac cgaatggaaa tcaggtcttg aagaaatact
      781 gaaaaacagt ccggaagagt tccagggata cgctggattc atacggagtc tggcagtgac
      841 tgcgccgtcg gcacaggcgg tgcttgctct tgtggacaag gattctcagc aattcactta
      901 cagaagcatc aagattcgcg agctaccgga caaagacttt cggaaactgt tgactgctta
      961 caatgataag ttgcccccga aactggcctc atttgcagaa gcgattcgac gttatctggc
     1021 atctcaccct gacatggatg gagcaaagtt aaaacaggag gttcttcatt tcttcagtcc
     1081 ttttcatccg gaaactacca gacgctaccg atcagacgcg gccaatcatc actacactgc
     1141 tttcatgaag ttcgatcatc agcacaagat tctgtacgaa gcaggatatc ggtacattga
     1201 ctatcgtcgt ttcatacaac ctgttggagt tcgcctgact ctcgtgttca ttacagtcgt
     1261 acttacgatc attttcggat tcagactttt cttcctcggg actctggtgc gtccgcttca
     1321 ggctcttctt tctggagtac gcaaagtaaa caaaggcaac ctggcagtac acgttccggt
     1381 tggtgtggcg gacgaaattg gatttctaac agagtctttc aacgggatgg tttcttctat
     1441 tagaattgcg cgcgaaaagt tggaacgtca tgcagatgag ctggaagtaa aagtgcgtga
     1501 aagaaccgcg gaactcacag aaacactgca gactgttcag gatctaaaaa gccagcagga
     1561 cggagactat ttccttactt ctttgttgat caaaccgctg gctagaaaca ccgctcgaag
     1621 tgaaacagtt cacgttgaat ttctcatgga gcagaagaag aaatttgaat tcagaaagtg
     1681 gaaagaggaa atcggaggag atttctgcag tgcgcatact gtggaacttg ccggcaaacg
     1741 ttatacggta ttggtgaacg cagatgctat ggggaaatcc attcagggcg ccggtggtgc
     1801 tctggtactg ggggcagttt tcgaatccat tatggagcga actcgttcta ttccttcttt
     1861 ccgtgatcaa tctccagaaa gatggctgaa gaatgcgttt caggaattgc atggcatttt
     1921 tgaaagtttt gacggaagca tgctcgtctc catggtgctt ggtttactgg atgatgaaag
     1981 tggtttgctg tactacgtga acgtggagca tccctggact gttctgtatc gtggtggcaa
     2041 agcaagcttc attgaaacag agcatcagtt tcgcaagctt ggtgtggcgg gcgtagaagg
     2101 tcagattcgc gtacagacat tccagatgga accagacgac gttttgattc tcggttcaga
     2161 cggacgcgac gacattctcc tgggtacttc tgaatccgga gaaagaatca tcaatgaaga
     2221 tgagcattta tttctcaaga tggtagaagc agcccagggt cgccttcctg aaattcgctc
     2281 tcacctggaa gaacagggcc cacttacgga tgatctttct ttgattcgcg ttgcctatcg
     2341 ttcgcatgaa gaggaccagg caccggacag agaagatgct atgactgcct atcgcgagaa
     2401 agctcgccag cttatggaag aaaataaatt cgcggaggcc gcagtcgctc tcggagatgc
     2461 actgcagctg gatccgcgta gtctcaaaac aatccgtatg atggtccggt tgttcctcga
     2521 tcaaaaagac tatgatcgcg ctttcgcgca ggtggaagac tacatttatc tgcgtcctct
     2581 ggatacagac atgatctatc tggcaagcta cctctgcaag aagctcaaga agctgcgtga
     2641 tgcggcagaa tttggagaga gagtgcggct gcgtgcgcct gacatggtgc gcaatctact
     2701 caacctgtct gaagtgtacg cgaggcttgg gaataaggat cgctctgcga agatattgcg
     2761 agaggtgtac aagctcgagc cggataacaa gaaagcagac aagttgcgca agcttctgga
     2821 aacagcggcg tagcgcggaa ctatccgcgc ttcttttcgt tgtcgtctaa cgtctcaagc
     2881 atgactttca ttggtctttc aatgacggtc cttacatgtg ccgatagctg ctcttgatcc
     2941 atcttgccag attcttccgg agagatggga tcgtgcactt ccacctggac cagtccagga
     3001 taaattctct gcacccatac tttttgcttc gcgggcataa tgtggtgcgc gccccagatg
     3061 gtaatgggct ggatggttac gtttgccttc tttgccagta cgaatgcgcc ctttttaaag
     3121 gggagcattc cctcgtgcgg attttcgctt ctggttcctt ccgggaaaat gattacggaa
     3181 gcgccgccct ggattctgtc tgccgcctgg aagagagatt ccattgcctt cttgcgatct
     3241 cccctttcta ccggaatgta tcctgctccc gtcatgcccc agccaacaaa aggaattttg
     3301 aaaagggacg cttttgccat ccaacggaat tcagtgtcga ggaatgagta gaatacagca
     3361 atgtcgaaca tgctctggtg attggccagg taaatgaccg gaccgccgcg gtagagtttc
     3421 tctttgtttt tcacctggat ggtcatccgt gaaagaaaag cgagactctt cccccacatg
     3481 cgtgcaccaa agtgtactgc ttttctggcg cggaaaagag cgaatggcat aacggatata
     3541 ccaaagaaca gtgttaccgt cagcaggatg gcccacagca caacagagta gatgaggtta
     3601 aagacgttag gcgttttgat catcttgaag tgtgatcttc gcgcgacggg aactcctggt
     3661 caatccgaaa acggtgattg tgttgtttca atccgttcta gctgttctac cagatcgtcg
     3721 atttgtgtgg cccagtattg ttccgtgccg aatgccggaa atgctccagg gaaagaaggg
     3781 tcttcccatc ttcgggcgat ccaggcgctg aaatgaatgt accggagtgc gcggagatgt
     3841 tctaccagct tgagactgcg acgatcgaat gtccgcatct gctcgtagcc gttcacaata
     3901 gcctcaagtt cttccgaatc ggaagcaagc agccacagat cctgaacagg tggcccactg
     3961 accatgtcat caaagtcaat cagaaatggt ccggcgttgc tccacagtat gttgccgccg
     4021 tggcagtctc cgtgcagcct gatttcttct agtgccggca ggaattcatt gggagcggaa
     4081 agctcttctc tcaagtttgc ggtgtgctcg caaatatctt ccaccaattg cgcataacgt
     4141 tcttccatgt gctccggtat ggattccgat tcaagtagat agtccaggtt ttgaagtccg
     4201 tatgtttctt cgttcagttt aattctgtga actgattttg atgcagcccc aacgttatgc
     4261 acccttgcca ggagtctgcc cagttgttct ctttctgtgc ctcccagttc atctaactga
     4321 cggccgccca ctctttcaaa aagagaaaag aagatgccac tttctgtttt gcccagtgtg
     4381 ctgccgccgg ggagcgctat aggcggcgcg cacgggattt cctgggctcg taaatctgaa
     4441 atgaattggt gctcttcgag gatttgctct cttgtccagc ggcctggtct gtagaacttc
     4501 gcaattcgga atcgttctga tttgttttcg ctttcaatgt cgatttctac ttcgtaaact
     4561 cgattctcca gggagtttcg ctgcagacat ctacctgtaa cgggaaggcc ggctgcttcc
     4621 acagatgcaa gtatggattc aggagtcagg ctgtagaaag attctccttc catggtgtca
     4681 gattgatgac gcagtcctgg cgggctttcc aaatccgatt gacctcctgg aaaaaaacag
     4741 gcattctgta tttatgaacg aaccgtcgca gctgctctat tttggagatc cgatgtgctc
     4801 ctggtgttac ggatttgctc ccgttctatt gcgactcaaa gaagatctgg agggaagact
     4861 tccgattcat tgcgtgctgg gcggacttcg cccgggccct catgcggaga aaatggacga
     4921 aaaattaaaa cgatttctcc gccatcactg ggaagaagtc catcgcacta cgggtcagga
     4981 gttcgggtat aagatctttg atcgcgacga ttttgtatac gacacggaac cttcctgtcg
     5041 cgcggtggcg attgtgcgaa agctgaagcc agacgctgtg atcgactttt tcgcacaggt
     5101 gcaaacagcc ttttacagag atagcaaaga taccaccatg cttgagacct acatccctct
     5161 ggctgcggag atcggagttt cgcctgagga atttactgcg ctctggaaca gtgaagacat
     5221 gaaagcggag acctggaagg attttgaatt cgcagcgtct gcgggcgttc gcggttttcc
     5281 aactctcatc tatatcgggg agggaagagg gcagatcatt tgcagaggat atcttccata
     5341 tgaagatgtg cgagagaggg tggagacagt tttgactgcg cgaaagcctg tactcgctcc
     5401 cacgcaggaa gcgtgcgata tcgaaaaagg aatttgttaa cccggcacta tcggcgcatg
     5461 tccatcagga agccgcgaat ctccgcgaga gatttcatca tagaaaaagc agagttctcc
     5521 ggcgaaagca tgacgagcgc catgcgatgc agcttttcat caatgatgcg aacggtagtg
     5581 agttcgtttt gagcgcgaga aaatgtagca ggtctggttc gcttttctac tttcattgct
     5641 tcgtccagag tttgtctggc aatcgcccgc accagatcct ggtaagcagc caggttgtct
     5701 ttggatggat tgtcgatcag gtctcgttcc gcctgaggca tctgagacca cagattctgc
     5761 agcgaagctc tttccggagt gctcgcgggc aacacttccg tcagcaaatc gaggaagctg
     5821 tttttctctg cagtcgtcga ttcgcctgtg cttgtgcgcg cggagccggc cgctcgccct
     5881 ttgagggatc cggtttgaat gcctactttc actggttcga agatcggcag aattgctcga
     5941 atgcagtaga acttttctgc ttttggatgg a
//
LOCUS       DAOQJL010000195         5964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7561_length_5964_cov_4.546455, whole genome shotgun sequence.
ACCESSION   DAOQJL010000195 DAOQJL010000000
VERSION     DAOQJL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7561_length_5964_cov_4.546455"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..568
                     /gene="ndhC"
                     /locus_tag="PLG78_09810"
     CDS             203..568
                     /gene="ndhC"
                     /locus_tag="PLG78_09810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE23453.1"
                     /translation="MNPVLEQYWPFAVFFVVAMLVAVGGLVVQSFLGPKLSSKHKFDP
                     YECGLDQMDSPHKPYSLKFYVVALLFMLFDIETIFLLPWAVGYRSFGWESLIAVIIFS
                     GIVVFGLWYVVKLKVFEWD"
     gene            559..1053
                     /gene="nuoB"
                     /locus_tag="PLG78_09815"
     CDS             559..1053
                     /gene="nuoB"
                     /locus_tag="PLG78_09815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNE23454.1"
                     /translation="MGLNPSTDFATSRIDAIVNWSRANSLWPMPFGVACCAIEMMASY
                     MPKYDTSRFGAEVLKFSPRQSDLLIVSGRISLKMMPVLTRIWEQMPEPKWCISMGACA
                     SSGGVFDTYALIQGIDQFIPVDAYIPGCPPRPEAFIDAVMMIQEKAKKGQARSQDYVD
                     RAVV"
     gene            1055..1588
                     /locus_tag="PLG78_09820"
     CDS             1055..1588
                     /locus_tag="PLG78_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE23455.1"
                     /translation="MIQFQASNFDTVKTRLESLGATNIHHDGASVVAEIPRTKAFDII
                     KKIKDDPEIGFRFFTDITGADFLKYSVKKPERYAVIYVLLSPSLGVRLELIAYLPEND
                     PSIQSIASLFTGANWAERETYDMYGFEFKGHPNPGRLLTPEGFEGHALRKDYPLKGKG
                     ERSSFPVYRATPGHASE"
     gene            1585..2574
                     /locus_tag="PLG78_09825"
     CDS             1585..2574
                     /locus_tag="PLG78_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE23456.1"
                     /translation="MKFRVLSSLRSFSRSTSFTLAGGVAFLLVMAGTSFFVQKVFAQV
                     PAPRFREATVLVPRGSIYYKVMGKGQPIILVHGGPGLDHRYMFQAFQDLAKDYRVIFY
                     DQRGTGASQCKLSDRSVTIEQFVEDLDAVRRHLGLRRINLLGHSWGGLLAVHYTVRHP
                     QNVRSLMLANSSGLTGESQKAFFENLDSMRTPEDKEELENLKGKSDQESLQRHNELYM
                     RPYFYDSSKIALLSYEPSKLTAENNERVRTLIWRQLLDYDLRPQASRLHIPTLVIHSD
                     RDVIPLEVAEETAETIPRSELVVMQDVGHYSFIEDPTEFFKEIRKFLKTVHGP"
     gene            complement(2580..4730)
                     /locus_tag="PLG78_09830"
     CDS             complement(2580..4730)
                     /locus_tag="PLG78_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE23457.1"
                     /translation="MRRVYKIVRIPLLLLVVGLIWSNVRYGIINRPLPVDPAWTAQAK
                     NVTIIRDEWGVPHIKGKTDADAAFGLAYAHCEDDFKFIQGGMVAARGDLARLVISKEA
                     VVNDFYVRLFKIRERADRDYDKISPDFRKVLEAYANGVNYYAATHPSEAKSKFFPASG
                     KDIAAGFIHKIPLFIGADKAMQKIMGDQPLRVDDPLDNAVAFQGLPVGSNAHALSHTR
                     TADGATYLNINSHQPWEGPVAWYEAHVQSEEGWNMTGGTFPGAPFILHGHNDHLGWAH
                     TVNYPDLVDVYKLKLDEQEDQYMFDGQLRPLNKTSVRIQVDIGLFDLPIPMSTYESAQ
                     GPVLKSDHGKYAVRVSGIDRLIFAGEQWYRMNKARNLTEWKKAMQMQAIPMFNAVYAD
                     ASNIFYVYNASLPTGRVPNQSYRKVLPGDTSQNIWDDKILPFAKLPAVENPPSGFIQN
                     CNSSPFETTTGPGNPDPGRYSLSMGIEKRSTNRSMRSRELLSKPGKISFEDFLRMKWD
                     RTYAPRSPIYEYAINPLLRDFKPQNEDESQALELLRKWNGRADDSPGSSLAILVYRPI
                     WQQTDLDHGPIPDRAILLRDAISFLKKHYGKLDVPLSILQRMRHGHLDEGLVGGPDVL
                     NAAHSLEEKDHIVGVAGDSYVLIVQFQPANVKSWSIHQYGNSTHKGSAHFADQMPLFL
                     KRTLKSTFRDVRDLNEHTECQYHPGGQSCAEARD"
     gene            complement(4754..>5964)
                     /locus_tag="PLG78_09835"
     CDS             complement(4754..>5964)
                     /locus_tag="PLG78_09835"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNE23458.1"
                     /translation="HLLVFTESARTSLYFQDQDITNISSAIANTATGHGLLPTAFLQT
                     GKAGSYLGHHFAPALLFFAPIYAAFPQTDHSIYPRILWIVFTMGAWIWMKIVHKSLPE
                     NKQGIVLFLIFSAFPVQRVEQSFHNEILVILFSGIAFLGLVTNRKFWFWFGIILWMTV
                     KEDMAVYTGLLGAVTFVWPHPHLNRREGAILSVCSLVWLIGSRLAQSHFGGGEGPAWS
                     MFFNADWPAQSLQPVTAKWKATVLVLGAFGFLAFRRIRYAALIVPIMMIHLVSQHPWH
                     STYYGHYAYSFLPFLMLTATEAAKETRLTWLLVLTTAACYINAGDRETPSPPFKADPA
                     YESLREKAQSLPADVCIHAEKHLSALVPVSVLVFPLEPFPGNPHRNGFGLDQECKKHI
                     LFEPNQPVDK"
BASE COUNT         1404 a         1460 c         1577 g         1523 t
ORIGIN      
        1 tcaggcttgc gactctctat catcagattg gcaaacatga tcgtgcatct gcgatccttt
       61 ctggatacag ggaggccttt cccgacgatc caaagggcgc tgcactcagt acattgctcg
      121 ggtccgccag ggttggcccc ggcgcaagct aaactgttct ttttctattt acagaaccct
      181 cgattcgaag gaacttgcgc ctatgaatcc ggttctcgag cagtactggc cttttgctgt
      241 ttttttcgta gtcgccatgc tggtggcagt gggtggcctc gtggttcagt cgtttcttgg
      301 cccaaaactt tccagcaagc acaagttcga tccatatgaa tgcggcctgg accagatgga
      361 ttcaccgcac aaaccgtatt ctcttaagtt ttacgtggtc gcgctcttat tcatgctctt
      421 cgatattgaa acgattttcc tgttgccctg ggctgttggc tatcgttcct ttgggtggga
      481 gagcttgatc gccgtaatca ttttctctgg catcgtagtc tttggtctct ggtacgttgt
      541 gaagctgaag gtgttcgaat gggattgaat ccaagcacag atttcgccac ttctcgtatt
      601 gatgcgattg taaactggtc gagagcaaac agcctgtggc ctatgccatt cggtgttgcg
      661 tgctgcgcga ttgagatgat ggcctcttat atgccaaagt atgacacttc gcgatttggt
      721 gcggaggttt tgaaattctc tcccaggcag tctgatttgc tcattgtctc cggccgcatt
      781 tctctcaaga tgatgccagt gctcactcgc atctgggaac agatgcctga accaaagtgg
      841 tgcatttcta tgggcgcatg cgcgtccagt ggcggagtgt ttgatacgta tgctctgatc
      901 cagggaattg atcaattcat tcctgtggat gcatacattc ctggatgtcc acccaggccg
      961 gaagctttca ttgatgctgt gatgatgatt caggaaaaag caaaaaaagg ccaggcccgt
     1021 tctcaggact acgtggatcg ggcggtcgtt tgagatgatt cagtttcaag cctcgaattt
     1081 tgacactgta aagaccagac ttgagtctct cggcgcaacg aacattcatc atgacggtgc
     1141 ttccgtggtt gcagaaattc cgcggacaaa agcattcgat attattaaga agatcaaaga
     1201 tgatccggaa ataggatttc gctttttcac ggatataaca ggtgccgatt tcctgaagta
     1261 ttctgtgaag aaaccggaac gttatgcggt catctatgtt cttttgtctc catctcttgg
     1321 cgtgcgcctt gagctaatcg catatctacc cgaaaacgat ccaagcatcc agagcatcgc
     1381 atctctgttc actggcgcca actgggcaga acgcgaaaca tacgatatgt acggctttga
     1441 attcaaggga cacccgaacc ccggcaggct tcttactccg gaagggttcg aaggccatgc
     1501 actgcgcaaa gattatccac tgaaaggcaa aggcgaaagg tccagcttcc ccgtttatcg
     1561 ggctactccc ggtcacgcgt ccgaatgaag ttcagagttt tatcaagcct gcgttcgttc
     1621 tccagatcca cttccttcac tctcgcgggg ggagtcgcgt tcctccttgt gatggctgga
     1681 acgagcttct ttgtgcaaaa agttttcgca caggtgccgg ccccgcgttt tcgcgaagcc
     1741 acggtcctgg ttccgagggg atccatctac tacaaggtaa tgggcaaagg ccagccaatc
     1801 atactcgttc acggcggccc ggggctggat catcgctaca tgtttcaggc atttcaggat
     1861 ctcgcgaaag attaccgcgt gatcttctat gatcagcgag gaactggcgc atcacaatgc
     1921 aaactctcgg atcgatctgt aaccatagag cagtttgtgg aagatctcga tgcggttcgc
     1981 aggcacctcg ggcttcgtcg tattaatttg ctgggtcatt cctggggtgg actgctggcg
     2041 gttcactaca ctgtgaggca tccgcagaat gtaagatctc tcatgcttgc gaattcatcc
     2101 ggcctgaccg gggaatcgca gaaggcattt tttgagaatc tggatagcat gcgaacaccg
     2161 gaagacaaag aggagctgga gaacctgaaa ggaaagtcag accaggaatc tctccagaga
     2221 cacaacgaat tgtacatgcg gccctatttc tatgactctt cgaaaattgc cctcttgtcc
     2281 tatgaaccaa gcaaactaac cgctgaaaac aatgagcgag tgagaacttt gatctggcgt
     2341 cagcttctgg actacgacct tcgtccgcag gcttcgcgtc tccacatccc cactctagtg
     2401 attcactcgg atcgagatgt gattcctctt gaagtcgcag aagaaacggc ggagacaatt
     2461 ccgcgttccg agttggtagt gatgcaggat gtgggacact acagtttcat tgaagaccca
     2521 actgagtttt tcaaagagat ccgcaaattc ctgaagaccg ttcacggtcc gtgatcggcc
     2581 taatcacgag cttccgcgca gctctggcct cctggatggt actgacattc tgtatgctcg
     2641 tttagatcgc gtacatcgcg aaaagtagat ttgagagttc gcttgaggaa cagaggcatc
     2701 tggtctgcga aatgtgccga acctttgtgc gttgaatttc cgtactgatg gatagaccag
     2761 gatttcacat tcgctggctg aaattgaacg atgagcacat aagaatcccc ggcaactccc
     2821 acgatgtgat ctttttcttc aagcgaatgg gcggcattga gcacatccgg gccacccacc
     2881 agcccttcgt ccagatggcc gtgtcgcatt ctctgcaaaa tagagagagg tacgtccagt
     2941 tttccatagt gcttctttag aaatgaaatc gcatctctca gtagaatggc tcgatccggg
     3001 atgggaccgt gatctaaatc cgtttgctgc cagatgggtc tataaaccag aatcgcaaga
     3061 gaggacccgg gagaatcatc cgcccttccg ttccactttc taagcaattc gagggcctgg
     3121 gattcatctt cgttctgcgg tttgaagtct cgaagcaacg ggttgatcgc atactcgtag
     3181 ataggcgatc ggggagcata ggtcctgtcc catttcattc gcaagaaatc ttcgaaactg
     3241 atcttgcccg gcttcgaaag tagttcgcgc gaacgcatgg aacgattggt gcttctcttc
     3301 tcaattccca tggatagcga ataacgtccc ggatcaggat tccctgggcc tgtcgttgtt
     3361 tcaaatggcg acgagttgca gttttgaatg aatccggaag gaggattttc tacggcaggg
     3421 agttttgcga aaggcaggat tttatcatcc cagatgttct gacttgtgtc tcccgggaga
     3481 actttgcgat acgattgatt cgggactctg ccggtgggca atgacgcgtt gtagacatag
     3541 aaaatattgg aagcatccgc gtacacagca ttgaacattg ggattgcttg catctgcatc
     3601 gcttttttcc attcggtgag attgcgcgct ttgttcatgc gataccattg ttcgcctgca
     3661 aagatgagtc gatcgatgcc agagactcga acagcatact ttccatgatc ggacttgagc
     3721 acggggccct gtgccgactc atacgtggac atggggatgg gtagatcaaa aagtccgata
     3781 tcgacctgga tgcgaacgct cgttttgttc agaggacgca gctgcccatc gaacatgtat
     3841 tgatcttctt gttcgtcgag tttgagtttg taaacatcca caaggtcagg atagtttaca
     3901 gtgtgcgccc acccgaggtg atcgttgtgc ccgtgcagaa tgaaaggcgc tccaggaaaa
     3961 gtgccccccg tcatattcca cccttcctct gattgtacgt gagcttcgta ccaggcgact
     4021 ggaccttccc acggttgatg tgagttgata ttgagatagg ttgcgccgtc tgcggttcgc
     4081 gtgtgcgaaa gagcgtgagc gttcgaacca acgggaagcc cttgaaatgc cacagcattg
     4141 tcgagaggat cgtccacgcg cagtggttga tcgcccataa tcttttgcat tgctttgtct
     4201 gcgccaatga acagcgggat tttgtgaatg aacccggcgg caatgtcttt gcccgatgcg
     4261 ggaaagaatt tgctttttgc ctctgatgga tgggtagcgg cgtagtagtt gactccgttc
     4321 gcgtatgctt ccagtacttt gcgaaagtcc ggcgaaatct tatcgtagtc tctgtccgcg
     4381 cgttcgcgaa tcttgaagag ccgcacgtag aaatcgttca ctaccgcttc ttttgaaatc
     4441 acgagtctgg ccagatcgcc gcgcgcggcg accattccac cctggatgaa tttgaaatcg
     4501 tcttcgcagt gcgcgtaagc gaggccaaat gcagcatccg catccgtctt cccctttatg
     4561 tgaggaacac cccattcgtc gcgaatgatg gttacgttct ttgcctgcgc ggtccatgca
     4621 ggatcgactg gcaaaggccg atttatgatg ccgtatctga cgttagacca gatgagaccc
     4681 acgaccagaa ggaggagcgg gattcgcacg attttgtaaa cgcgtcgcat aacagggatt
     4741 tagccctgat tgctcatttg tcaacaggtt ggttgggttc aaacaggatg tgtttcttgc
     4801 attcttgatc caggccgaat ccatttctgt gcggattgcc cggaaacggc tcgagaggaa
     4861 agaccagaac actcacagga acgagtgcgc tgagatgctt ttctgcgtga atgcatacat
     4921 ctgccggcag gctttgggct ttttcgcgca aagattcata ggctgggtct gctttgaaag
     4981 gaggtgaggg agtctcacga tctccggcgt tgatatagca ggcagctgtc gtaagcacca
     5041 ggagccatgt gagcctggtt tctttcgccg cctccgtcgc ggtaagcatg aggaagggca
     5101 gaaaagaata agcgtagtga ccgtagtacg tagaatgcca tgggtgttgc gaaaccaggt
     5161 gtatcatcat gatgggcacg atcaatgccg cataacggat tctgcgaaat gcgagaaaac
     5221 caaaagcgcc cagcacgagc acagtggctt tccatttcgc agtgacgggc tggagggact
     5281 gtgccggcca atctgcgtta aaaaacatgc tccacgccgg tccttcgccg ccaccgaaat
     5341 gcgactgagc caggcgactt ccaatcagcc acaccaggct gcaaacagag aggatcgcac
     5401 cttcgcggcg gttcaagtga ggatgtggcc agacaaaagt gactgccccc agtagaccgg
     5461 tgtaaaccgc catgtcctcc ttgacagtca tccagagaat gattccgaac cagaaccaga
     5521 attttcgatt tgtgaccagt cccaggaatg cgattcctga gaaaaggatg accagtattt
     5581 cattgtgaaa tgattgctct actctctgca caggaaacgc ggagaagatc aagaacagga
     5641 cgattccctg tttgttctct gggagagatt tgtgtacgat cttcatccag atccaggcac
     5701 ccattgtaaa tacgatccag agaattcttg ggtagatgct gtgatctgtt tgcggaaacg
     5761 ctgcatagat cggagcaaag aagagaagcg ccggagcaaa atgatgaccc agataactcc
     5821 ccgctttccc tgtttgtaga aacgcagtgg gcaggagccc gtgaccggtg gcagtgtttg
     5881 caattgcaga agatatgttg gtaatgtcct gatcctggaa atagagagac gtgcgcgcgc
     5941 tttccgtgaa cacaagaaga tgaa
//
LOCUS       DAOQJL010000196         5959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7575_length_5959_cov_5.015752, whole genome shotgun sequence.
ACCESSION   DAOQJL010000196 DAOQJL010000000
VERSION     DAOQJL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7575_length_5959_cov_5.015752"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLG78_09840"
     CDS             <1..371
                     /locus_tag="PLG78_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE23459.1"
                     /translation="EVIFNAPDFALEDFKKAAPILRPAVKRITLYCSPGDNALVASEK
                     VNGGKRIGRCAKIPDVDVVNVNEVDSPVLGIGGLGHGYYSGRAILTDVYQVLLGVDVS
                     RRLFVRKSSEYGGEDYVLRR"
     gene            complement(353..1765)
                     /gene="rsmG"
                     /locus_tag="PLG78_09845"
     CDS             complement(353..1765)
                     /gene="rsmG"
                     /locus_tag="PLG78_09845"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNE23460.1"
                     /translation="MALSASRLADDLADAGIELDSHQAKQLLRFYELVEERNPDLNMT
                     RIRGYEATVRKHYVDSLIILRYLKRIFPPLGLPASILDFGTGAGFPGVPLAIACPETR
                     VILNESRANRAEFLEDVVEECGLKNATVLGKRLAMEDDIHVDAVIARAVSTMADLLTR
                     TIFSIKKGGSIIYMKGPNCDAEIAEMGAGVSLLLDQSYTLPASEDHRRLVCFRVDDPG
                     EWKAPGSNAISSRENEKFKMLISLQSSRGIRKNGLCLIGGKKIVSEVVSNARVRVQAA
                     VVPQSFSGSMPPDVRQFEFKNDLFAQIDELGTGAPLLLAEIPEMSEWDGRVKGLCVVI
                     PFQDPENVGAAIRSAVAFGAGRIILTKECANPFLARAIRSSAGAVFVAPLVKGPSLVE
                     LLENYPEIYQLSSDGIALEDVNFSKDILITAGLEGPGLPGAGGNRLRIVMRSDVESLN
                     AATALAIALYEFDRSQRRKT"
     gene            1915..3072
                     /locus_tag="PLG78_09850"
     CDS             1915..3072
                     /locus_tag="PLG78_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE23461.1"
                     /translation="MFEWVHYQFPARIFIEKNATHKIGTLVKEVGSRALLVALKSEMS
                     NPDELAVIKTSLDKYTSGCILYDDMASRPGNKELDTAAHFAKQSRADIIIGYGGRDSF
                     HAAKAIALLCKNDVFAEDLPHTALPLKRAPLPVAQVPAAPSMGEETSPVFSVYNPETR
                     SAFFSQDYRLFPAIIFVDPNITSTLSKGELSRAGAANVAAAVESILSRRANDITTSIA
                     LRSLDLVQKNLQQVMKEPGNMAARTNVSLASVLCGMAHSNSMLGLCYSIASATSLLTG
                     LEFHLAMALLLPHVMEYNLTTSAGRYVQIARALDEDIKDITVIEAAIKAVEGVRKTYL
                     ELKIPQRLSEFEIAKEDLPEIAELAFRIPMVKNSPRDLDKNEIETILIAAY"
     gene            complement(3084..4127)
                     /locus_tag="PLG78_09855"
     CDS             complement(3084..4127)
                     /locus_tag="PLG78_09855"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE23462.1"
                     /translation="MKIFITGGAGFLGYHLVQRLKKDKHKITLYDIADFNPADYPKDV
                     KMIKGDVRDAKALQEAMKGHDAVIHAAAALPLWKKEDIYSVTIDGTRNVLDSAAVNKI
                     DRVVYISSTAVYGIPKKHPILETDPRVGVGPYGEAKIEAEKICEEYRHRTKMCICVIR
                     PKTFIGTGRLGVFQILFDWVESGVKIPVFGNGKNRYQLLDVRDLCDAIELGMTGNAKK
                     ANDNFNVGAEKFQTVREDVGALCAFSKSGSRVFGVPSSPLKIALRFFEILHISPLYRW
                     VYGTADKDSFVSTEKLTKALGWHSKYSNSDALIDSFRWYLVNKPQDADSVAGVTHRVP
                     WKQGILKVFKKFL"
     gene            4211..5215
                     /gene="ilvC"
                     /locus_tag="PLG78_09860"
     CDS             4211..5215
                     /gene="ilvC"
                     /locus_tag="PLG78_09860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNE23463.1"
                     /translation="MAQVFYDDSCDSKFLKGKTIAVLGYGSQGHAQAQNMRDSGLNVI
                     IGLKADSKSAPIAREDGFEVLSPFDAAAKADIIQLLAPDQVQMTIYEESIKPNLKKGK
                     ALVFSHGFNIHYELIKPPADVDVYMVAPKGPGHLVRRVYREGGGVPCLIAVHQDATGE
                     AKQRALAHASGVGGGRAGILETNFKNETETDLFGEQVVLCGGLTSLILAGFETLTEAG
                     YDPDIAYFECLHEVKLITDLIYEGGIARMRHSISDTAEYGDLTRGPRIVDAGVKARMK
                     EVLTEIQKDKGAKFATEWLAESKNGYPNFNKLREAGLKHPIEQVGQKLRGMMKFLGKK
                     "
     gene            5225..>5959
                     /locus_tag="PLG78_09865"
     CDS             5225..>5959
                     /locus_tag="PLG78_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23464.1"
                     /translation="MSLLYDARLVENSGIGTTIRGQLREILSRKIPITLTGNSRQLAS
                     HSLTGVPVIPFDAKLYSIKEQTSFPRASHARIHVPHYNAPLRYLSHCSVTVHDLIHLQ
                     SDEFKHPAYRAYASLMLSQIARRSPCILTVSETTRSQFLSRYPEARHRTFTTLNGIDH
                     KLFRPASSGAVAALKKKHSLPDEYFLAIGIGKKHKNIDFALRALKHLWKDTDCPPIVL
                     AGAGGKIPSYATHAMMENSVRKNVILL"
BASE COUNT         1575 a         1582 c         1384 g         1418 t
ORIGIN      
        1 gggaagttat attcaacgcg cctgactttg ctctcgaaga ttttaagaaa gcagcaccca
       61 ttcttcgacc tgcagtcaag cggatcactc tctattgctc tccgggagac aatgctctcg
      121 ttgcctctga aaaagtaaac ggaggtaaac gaatcggcag atgtgcgaag attccagatg
      181 tagatgttgt aaacgtaaac gaagtagatt ctcccgttct aggaatcgga ggactcggac
      241 acgggtacta ctcaggtcgt gcgattctca cggacgtata tcaggtacta ctcggagtgg
      301 acgttagccg gcgactcttt gtgcgcaaaa gtagcgaata cggaggcgaa gattacgttt
      361 tgcgacgctg acttctgtca aactcataaa gcgcaattgc aagagctgtc gcggcattca
      421 gtgattccac atctgatcgc ataacgatgc gtagacgatt gccgccagct ccgggtaaac
      481 ccgggccttc cagacccgct gtgatcagaa tatccttgct gaaattcaca tcttcgaggg
      541 cgattccgtc agacgaaagt tgatagattt ccggatagtt ctcaagcaat tccacaagag
      601 aaggtccctt caccagtggg gccacaaaaa ctgctcccgc agaggaacga atggcccgcg
      661 caagaaaagg attcgcacat tcctttgtga gaatgatcct gcccgcacca aaggcaaccg
      721 cgctcctgat ggcagcacct acattttccg gatcttgaaa gggaatcaca acgcaaagac
      781 ctttcaccct tccatcccat tcggacattt cgggaatctc cgcaagaagc aacggggcac
      841 ctgtacccaa ctcatcgatc tgtgcgaata gatcattctt gaactcaaat tgccttacgt
      901 ccggcggcat gctcccggaa aagctctgag gaaccaccgc cgcctgtact cgcacgcgcg
      961 cattggacac tacttccgaa acaatcttct ttccgccaat aagacaaagt ccattctttc
     1021 tgataccccg ggaactctgc agagagatca acatcttgaa cttttcgttt tctcgggatg
     1081 aaattgcgtt cgatccagga gctttccact ctcctggatc atccacgcga aagcacacca
     1141 ggcgacgatg atcttccgaa gcaggcaggg tgtatgactg atcgagaaga agagacacac
     1201 ccgcacccat ctccgcaatt tctgcatcgc agttcggacc tttcatgtaa atgatggaac
     1261 cgcccttctt gatggagaag atggtccgag tcaggagatc tgccattgtg ctcaccgcgc
     1321 gtgcgatgac tgcgtccacg tgaatgtcat cttccatggc gagccgctta cccagaaccg
     1381 ttgcgttctt caatccgcat tcttctacca catcctcgag gaactccgcg cgattggctc
     1441 tgctttcgtt cagaatgact ctggtttcag gacaggcgat cgccagcgga actcccggaa
     1501 atccggcgcc tgtaccaaaa tcaagtatgg acgccggcaa tccaaggggt ggaaaaattc
     1561 tctttagata tcgaagaatg atcaggctgt ccacatagtg cttgcgtact gtagcctcat
     1621 atccgcgaat ccgcgtcatg ttcaggtctg ggttgcgctc ctcgaccagt tcgtaaaagc
     1681 gaagcagctg cttcgcctgg tgagaatcca gttcgatccc ggcatctgcc agatcgtctg
     1741 cgagcctgga tgcactgagc gccatagttc accgcacatt cctgaatgca caggtcaagc
     1801 aggagcgcga aatgagcgag ggattaaccg aaaacctccg atactatagc gggagcccat
     1861 cgtccatttt gaacaatcca tccctgtcac tatccaactg cgaggaagcg gcctgtgttt
     1921 gaatgggtac actatcagtt tcctgctcgc atttttattg aaaagaatgc gacccacaag
     1981 atcggcaccc tcgtaaaaga agtgggaagt cgagcgcttc tggtcgcgct gaaaagcgaa
     2041 atgtcgaatc cagatgaact ggcagtcatc aaaaccagtc tggacaaata tacgtcgggc
     2101 tgcatcctct acgacgatat ggcttcgcgg cctggaaata aggaactcga cactgcggcc
     2161 catttcgcga agcaatcgag agccgacatc atcatcggtt atggcggcag agactccttt
     2221 cacgctgcaa aggcaattgc tctcctgtgc aagaatgatg tcttcgcaga agatctaccg
     2281 cacactgcac ttccactgaa acgggcgcct ctgccagtcg cccaggttcc tgcggcgccc
     2341 tccatgggcg aagaaacgag tcctgtgttc tcagtttaca atccagaaac tagatccgca
     2401 ttcttttcac aggattacag actgttcccc gcgatcatat tcgttgatcc aaatatcaca
     2461 agcacactga gcaaaggcga actcagtcga gctggtgcgg caaacgtcgc cgctgccgtg
     2521 gaatcgatat tgtctcgacg tgctaacgat atcacaacgt cgattgctct gcgctctctg
     2581 gatcttgtgc aaaaaaactt gcaacaggta atgaaagaac ctggcaatat ggctgcacgc
     2641 accaacgtga gtcttgcaag cgtgctttgc ggaatggcgc attcgaacag tatgctcggt
     2701 ctctgctata gcattgcatc cgcgacgagc ctacttacag gcctggaatt tcatctggct
     2761 atggcacttc tacttccgca cgtaatggaa tacaatctta caacgtctgc ggggcgttat
     2821 gtgcaaattg cgcgagcgct tgacgaagac atcaaggaca tcaccgtgat tgaagctgcg
     2881 attaaagcgg tggaaggcgt ccgcaaaacc tacctggaat tgaaaattcc tcagagactg
     2941 agcgaatttg aaattgcaaa agaggatttg cccgaaattg cggaacttgc gttccggatt
     3001 cccatggtga agaactcacc acgggatcta gacaagaatg aaattgaaac aatcctgatc
     3061 gcggcctact aggccattca ttcttaaaga aatttcttga atactttcag gatcccttgc
     3121 ttccacggta cgcgatgcgt aacacctgcc acgctatctg catcctgcgg cttgttcaca
     3181 agataccacc ggaaagaatc aatgagcgcg tctgaattcg aatacttcga atgccatcca
     3241 agagctttcg tgagtttctc agtggagacg aatgaatcct tgtctgccgt tccgtatacc
     3301 cagcgataaa gcggagaaat gtgaaggatc tcaaaaaatc taagagcgat cttgagggga
     3361 ctggatggga ctccaaagac tctcgagccg ctttttgaaa aggcacacag agcgccgacg
     3421 tcttcgcgca ctgtctggaa tttttccgca ccaacattga agttgtcgtt ggccttttta
     3481 gcatttccgg tcattccaag ttcgatcgca tcacagagat ctcttacatc aagaagttga
     3541 taacggttct ttccgtttcc aaagacagga atttttacac cggattcgac ccagtcaaaa
     3601 aggatctgaa acacaccaag acggcctgtt ccaatgaatg tttttgggcg aataacacaa
     3661 atacacatct ttgtgcgatg acgatactct tcgcaaattt tctccgcttc gatctttgct
     3721 tctccgtaag ggcctacgcc cacacgagga tcagtctcaa gaattggatg tttcttggga
     3781 ataccataaa ctgcagttga gctgatatac actactcgat cgatcttgtt aacagcagcg
     3841 ctatccagaa cgtttcgcgt gccgtctatc gttacagaat agatatcttc ctttttccag
     3901 agaggaagcg cggcagcagc gtgaatgact gcatcatgtc ctttcattgc ttcctgcaat
     3961 gccttcgcat cgcgcacatc ccctttgatc atcttcacat cttttggata gtcagcgggg
     4021 ttaaaatctg cgatatcata aagagtgatc ttgtgtttgt ctttcttcag tctctgtaca
     4081 aggtgatagc ccagaaagcc ggcaccgccg gtaataaaga ttttcatagt attttggtta
     4141 attttttttg agaggccccc ctgtaaagtt tttccttgcc atcgcactga cctgaacgcg
     4201 ttgtgtccgc atggcacagg ttttttacga cgattcttgc gattctaagt tccttaaagg
     4261 aaaaacaatc gctgttcttg gttatggcag tcagggacat gcacaagcgc agaacatgcg
     4321 cgattcaggc ctgaatgtca tcattggcct caaagcagac tccaaatcag cacctatcgc
     4381 ccgcgaagat ggtttcgaag tactttcacc atttgacgca gccgctaaag cggacataat
     4441 ccagttgctt gcaccggatc aagtgcaaat gacaatctac gaagagagca tcaaacccaa
     4501 tctgaagaaa gggaaagcac tcgtgttttc gcatggcttc aacattcact acgaactcat
     4561 caaaccaccg gcagacgttg acgtttacat ggtcgctccc aaaggtcccg gtcacctcgt
     4621 gcgtcgcgtg tatcgcgaag gcggaggagt tccttgcctc attgcagttc accaggatgc
     4681 gacaggagag gccaaacaaa gagctctcgc gcatgcgtcc ggtgttggtg gaggtcgcgc
     4741 tggaattctg gaaacaaatt tcaagaatga aacggaaaca gatctcttcg gtgaacaagt
     4801 agttctttgc gggggactca catcactcat cctcgccggt tttgaaacac tgacagaagc
     4861 cggttacgat ccggatattg cttactttga atgtctgcat gaagtaaaac tcatcacgga
     4921 cctgatctac gaaggtggaa ttgcacgcat gcgccattct atctctgaca ctgcagaata
     4981 cggagacctc acgcgcggac cacgaatcgt ggatgctgga gtgaaagctc gtatgaaaga
     5041 agtactcact gaaatccaga aagataaagg cgcgaaattc gcgacggaat ggcttgcaga
     5101 atccaagaat ggttacccga acttcaacaa acttcgcgaa gcaggcctga aacacccaat
     5161 tgaacaggta ggtcaaaagc tccgcggaat gatgaaattc ctgggcaaaa aataacatcc
     5221 ggccatgagc ctgttatacg atgccagact cgttgagaac tccggcatcg gaaccacgat
     5281 ccggggtcaa ttgcgggaaa ttctttcccg caaaatcccc atcactctta ccggaaacag
     5341 cagacaactt gcatctcatt cgctcaccgg cgtccccgtc attccctttg acgcgaaact
     5401 atattccata aaggagcaaa catccttccc cagagcttcg cacgcacgca tccatgttcc
     5461 tcattacaac gcaccacttc gttatctgtc tcattgttct gtgactgtcc atgacttgat
     5521 ccatcttcag tcagacgaat tcaagcaccc ggcttacaga gcatatgcgt cgctcatgct
     5581 ttcgcaaatc gcacgccgtt caccctgcat tctaactgtt tctgaaacaa cgcgaagcca
     5641 gttcctcagt cgatatccag aagcacggca ccgcacattc acaacgttaa acggcattga
     5701 ccacaaacta ttcaggccgg ccagctccgg tgcggtcgct gcactcaaaa agaaacattc
     5761 tctccccgat gaatactttc tggcaatcgg cattggaaag aagcataaga atattgactt
     5821 cgccctgcgt gcgctcaagc acctctggaa agacacggac tgtccgccca tcgtgctcgc
     5881 gggagccgga ggaaaaatcc catcttacgc gacacatgcc atgatggaga actcggtcag
     5941 aaaaaatgta atccttctc
//
LOCUS       DAOQJL010000197         5940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7607_length_5940_cov_5.010195, whole genome shotgun sequence.
ACCESSION   DAOQJL010000197 DAOQJL010000000
VERSION     DAOQJL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_5940_cov_5.010195"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1617
                     /locus_tag="PLG78_09870"
     CDS             232..1617
                     /locus_tag="PLG78_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE23465.1"
                     /translation="MHPDQLRKLISLSDCFAEVRYHKKKSRTISVRKGEVVEMSLRQL
                     DGVSIRVLKNGTFGFASTSDMTDEGLARALKSAMEMARELQTRRLHPVTLAQANLARG
                     TFATDGYQTLDQMPVEEKLGIVRKCEEDLRKSSHIQGAQCQYAEIFEEKHIVTTDGAD
                     CIVQIVRPELRFSAFAEEGGKMTSGFDSVGATGGWKCLFENRSIESYIETAAKSAVDL
                     LGAPVAEGGKHTVILSPAMVGLLSHEAIGHTVEADFVKSGSVAQGKIGHMVASELVTL
                     CDSGLSEFKDHAGGMLPVDDEGVVTGNTTIIDKGRLVSYLHNRQSAAEFGAAPTGNAR
                     AWEYSDEPLIRMRNTYVAPGSSELDHMIGEIKEGFYIDGPEGGQADATGEFMFGASRV
                     RKIKNGKLEGMVQKVSVSGNAFEVLSSVDAVSRDFQWDLGSGHCGKGQPAKVDAGGPF
                     IRCKVLIGGEQ"
     gene            1614..2987
                     /locus_tag="PLG78_09875"
     CDS             1614..2987
                     /locus_tag="PLG78_09875"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE23466.1"
                     /translation="MTPEQLHQIEKELQQAAREALGEVGRADGEICCGYENKARIIFE
                     GNEFSLGAVNEERTFGVRVIDQGRMGFSSTNQPDHLLSTARVACEIARLSPASEFHCI
                     PDGTPHNVVSLIDSKLMETDSRDAGNLLEILVKAASSDKRVTIDRAEIALSTRIQSVL
                     NSKGVYSSYSITTAEYYIMGMARDGEEVTSFDYDGGVSAQRGDLESQIETTGLEFRSS
                     VIGSLHPIKGKSTRGAVLLHPRAVSDLFGSAVFFNTNARMHQDLTSSWRDKLNQSVAH
                     PSLMVTEHPLARDRAEGFMPFDREGVPASDHAIIDGGRLNFVAHNSFTARRGRSAPTG
                     NASGSPKSMPGIGFSNTSFGLKSGSVPIQTESEWLDDLKHALLLVRFSGNDDPVSGEF
                     SGVAKNSSWIEHGALRPASEVMISGNVFDLLKNIAGGTAQVFPVFGGSHAPYILVEGV
                     SITAGNE"
     gene            2984..3625
                     /locus_tag="PLG78_09880"
     CDS             2984..3625
                     /locus_tag="PLG78_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23467.1"
                     /translation="MSEKQTINLRVLDEKTKGVLRTTFDDLERQLKIDSLGLTLFYVV
                     MELVGNAVKANLKRVFFRTHSYNLSDPDDYRIHIDEFKAYYNAVSEEEYTRAFDDLGL
                     KVTIDIDLDHRRLLIFVENTATLHPEEEKRIRAKLASTREMKDILEFSVQFADETEGK
                     GLGLAMIVLLIRDLGFDPQYFRVYKTEDKTVARLEFPLSADYTPIRLRQSTLS"
     gene            complement(3607..4644)
                     /locus_tag="PLG78_09885"
     CDS             complement(3607..4644)
                     /locus_tag="PLG78_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23468.1"
                     /translation="MMQAYILEPDSNIQEYYRNLLGTELRGLTLHFFATGEELRRRIS
                     DQPCDLVILESMGNPAVVELIDELSSRKIPSIVVSEECTERLVVECLKAGALDFVSKR
                     NIKLGLLLDVVRRALLEADRWMDTRDLYSRLPVRPEFIRMNQSLRTSISKEQAEVRRM
                     RIKTGQDLGGTYEEGKSYNLIFVFLQLHFPETLARAGDDKSSKVVNEALDKIVSVPAR
                     YGGEVWVRRRDGGIFVFPAAQALAALLCAFEVRGLISMLNVTTENIYENIKANLALTS
                     GMTVYRRNVGDVHSDALNLAAHLAIQYPRSNAILMPSSLVEQLSERARKYFFKGETFE
                     GKEISVYERVL"
     gene            complement(4641..5591)
                     /locus_tag="PLG78_09890"
     CDS             complement(4641..5591)
                     /locus_tag="PLG78_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23469.1"
                     /translation="MFAKFFELFMAAENLKDIRKLVKEFLWKSLNTDQMNYVAKEADP
                     RFDLGKVSGFGHSIVVTRQTAADVIVDYFATEDAFLHLFATLLFMEGKNASGGVIHFV
                     NPDPIIKKLRERQWIYDPSAGRFIKSQSQTRTADWGFLQEGSEYSLVFGSIDIVGSSA
                     LARTNVPVDVENTLVRMRAYLQDQIEARNGRIWYWHGDGGVMAFYGADAASRAVISSV
                     AILAYLPIFNLTQNELRPENDVSLRIGLHAGLAVYRSDTEKILSPDIRFAEEVEKHCA
                     ETNTIAITERTVNSLPPEVRRQFNPAGEHSANRIYVYRPV"
BASE COUNT         1545 a         1493 c         1528 g         1374 t
ORIGIN      
        1 gcagtggcta acgctgagaa gacattcgaa ggcttcaaag caaaaggtgc cgcggatttc
       61 tcagacccgg ccatgaagac tcgcgagttc gtagcaactg cagttcgcac tgtagctggc
      121 gacaagtaat cgctccctgg ccgggcatcc gcccggccgt gatttttttt ctactgcagg
      181 atctgttatt tttgcctggc acaccgcacc ccttctcact ttcttaccaa cgtgcaccca
      241 gaccagcttc gaaaactcat ttcactctca gattgtttcg ctgaagtccg ttatcacaaa
      301 aagaaatcca gaaccatttc ggtccgcaag ggagaagtgg tagagatgag tctgcgtcaa
      361 ctcgatggag tatccattcg agttctcaaa aatggaacgt ttggattcgc atccacttcg
      421 gacatgacag acgaaggtct cgcacgtgca ttgaagtctg ccatggaaat ggctcgtgaa
      481 cttcaaacgc ggcgtcttca ccctgtgact ctggcacagg ccaatcttgc gcggggcact
      541 ttcgcaacag acggctacca gactctcgat caaatgcctg tagaagaaaa gcttggcatt
      601 gtgcgaaagt gcgaagaaga cttgcgaaaa tcatcgcaca tccagggcgc gcagtgccag
      661 tacgccgaaa tctttgaaga aaagcacatc gtaactacag acggcgcgga ttgtatcgtt
      721 caaattgttc gcccggagct tcgcttctcc gccttcgcag aagaaggtgg aaagatgacc
      781 agtggatttg attcagtggg tgccacgggc ggctggaaat gtttgttcga aaacagatcg
      841 attgaatcct acatagaaac cgccgcaaaa agcgcagtcg atctactcgg agcccccgtg
      901 gcggaaggcg gtaagcacac agtaattctc tctccagcaa tggttgggct cctttcgcac
      961 gaggcaatcg ggcacactgt ggaagcagac tttgtaaaaa gcggcagcgt agctcagggt
     1021 aagatcggac atatggttgc ctccgagctc gtaacgttat gcgacagcgg tctgagcgaa
     1081 ttcaaagatc atgcaggcgg tatgctgccc gtggacgatg aaggagtcgt tacaggaaac
     1141 acgacgatca tagacaaagg cagactcgtc agctacttgc acaacagaca gagcgcagcc
     1201 gaattcgggg ccgcacccac aggaaatgca agagcctggg aatactcgga cgaacctctc
     1261 atccgaatga gaaatactta cgttgcgccc gggtcttctg aactcgacca catgattggc
     1321 gaaatcaaag aaggcttcta tatagacgga ccggaaggcg gacaggcaga tgcaacgggc
     1381 gaattcatgt tcggtgcgtc ccgcgttcga aaaatcaaga atggaaaact agaaggcatg
     1441 gtgcaaaaag tgtctgtttc cggaaatgca ttcgaagtgt tatccagcgt ggacgcagtc
     1501 agtcgcgact tccagtggga tctgggcagc ggccattgcg ggaaaggaca gcctgcgaaa
     1561 gtagacgcgg gaggcccgtt cattcgctgc aaagtgttga tcggcggaga acaatgaccc
     1621 ccgagcagct ccatcaaata gaaaaagaac tacaacaggc agccagagaa gcgctcggcg
     1681 aagtgggacg tgcggacgga gaaatctgct gcgggtatga aaacaaggcc agaatcattt
     1741 ttgaagggaa tgagttttca cttggtgctg tgaatgaaga acgtacgttc ggagtgcgcg
     1801 taatcgacca gggacgaatg ggattctctt ccacaaacca gcccgatcat ctattgtcca
     1861 cagcgcgcgt tgcatgcgaa atcgcaagac tctctcccgc ttccgaattt cactgcattc
     1921 cagacggaac gccgcataac gttgtatctc tcatagactc gaagctcatg gaaacggact
     1981 cccgcgatgc tggaaattta ctggagattc tggtgaaagc tgcgtcctct gataagagag
     2041 tcacgatcga cagagccgaa atcgcattga gtactcgcat tcagagcgtc ttaaactcaa
     2101 agggcgtcta ttcgtcctat tccattacaa cagcagaata ttatatcatg ggcatggcac
     2161 gggacggaga agaggtcacc tcctttgatt atgatggcgg ggtttccgca cagagaggtg
     2221 atctggaatc gcagatagaa accactggtc tcgaatttcg ctcgtctgtc atagggagtc
     2281 tccatcccat caaaggaaaa tcaaccagag gagcggtgtt gcttcatccg cgcgccgttt
     2341 cagatctatt tggaagcgct gtcttcttca atacaaatgc acgcatgcac caggatctaa
     2401 ccagcagctg gagggacaaa ctgaatcaat cggtcgctca tccctccctg atggtcacgg
     2461 agcatccgct ggcccgcgac cgcgcagagg gcttcatgcc ttttgacaga gaaggagtgc
     2521 ccgcatccga tcacgccatc atcgatggag gtcggttgaa tttcgttgca cataactcct
     2581 ttaccgcccg cagaggaaga tcggctccga ctggaaatgc atccggaagt ccaaaatcta
     2641 tgccaggaat cggattctcg aatacttctt tcggactaaa aagcggttct gtgcccatcc
     2701 agacagaatc ggaatggttg gacgatctca agcacgctct cctcctggtg cgcttcagcg
     2761 gaaatgacga tcctgtttcg ggcgagtttt ccggggttgc caagaattca agctggatag
     2821 aacatggagc attgaggccc gcaagcgaag tgatgatatc cggaaacgta tttgatctac
     2881 tgaagaacat tgccggcggc actgcgcagg ttttcccggt atttggcgga agccatgcac
     2941 cctacattct cgtggaaggt gtaagcatca cggcaggaaa tgaatgagcg aaaagcaaac
     3001 aatcaacctc cgcgtactgg atgagaaaac aaagggagtg cttcgcacta cttttgacga
     3061 tcttgaacgt cagctgaaaa tagacagtct tggactcacg ttgttctacg tggtcatgga
     3121 actggttgga aacgcagtca aagcaaacct caagagagtc ttctttcgca cgcacagcta
     3181 caacctgagt gatccagacg attacagaat ccatatcgat gaattcaaag cgtactacaa
     3241 cgccgtctca gaggaagaat acactcgagc attcgacgat cttggactga aagtcaccat
     3301 tgacattgac ctggaccaca gaagactctt gatctttgta gaaaacactg ctacacttca
     3361 cccggaagaa gaaaaacgca ttcgcgcgaa gctcgcaagc acgcgcgaaa tgaaagatat
     3421 tcttgaattc agtgttcaat tcgcggatga aacagaaggc aaaggcctcg ggctggcaat
     3481 gattgttctt ttgattcgcg atcttggatt tgacccgcag tactttcgag tctacaaaac
     3541 ggaagataag acagtggcgc gcctggagtt tcctctctcc gcggattaca cgcccatccg
     3601 gttgcgtcag agcactcttt cgtaaacgga aatttctttt ccttcgaacg tctccccttt
     3661 gaagaaatat ttgcgcgctc tttcgctgag ctgctccaca agagaagatg gcatgagaat
     3721 cgcattgcta cgcggatatt gaatggccag gtgtgcggcg agattgagcg cgtcactgtg
     3781 cacatctccc acgtttctgc gatatacagt cataccggaa gtaagtgcaa gattggcttt
     3841 gatattttcg tatatattct cagtcgtaac gttgagcatg ctgatgagac cgcgcacttc
     3901 gaacgcacac aaaagagctg caagcgcctg agccgccgga aatacgaaga ttccaccatc
     3961 tcgtcgcctc acccacactt ccccgccgta gcgcgctgga acggacacaa tcttgtcgag
     4021 tgcttcattg acgaccttgc ttgatttgtc gtctcctgcg cgtgcgagag tttcagggaa
     4081 atgaagttgc aaaaacacga agatcaggtt gtaggacttg ccttcttcgt acgttccacc
     4141 cagatcctgg ccggttttga ttcgcattct gcgaacttct gcctgctcct tgctgatgga
     4201 tgtacgaagg gattgattca tccgaataaa ttcggggcga acaggcaggc gagagtacaa
     4261 atcgcgagtg tccatccatc tgtctgcttc cagcaaagct cgacgcacta cgtcgagaag
     4321 aagcccgagt ttaatgtttc ttttggaaac aaaatctaga gctcccgctt tcagacattc
     4381 tacgaccaac cgctccgtgc attcttcgga tacaacgatc gaagggatct tgcgcgaaga
     4441 tagctcgtcg atcaattcta cgactgcagg gtttcccatg gactcaagaa tcactagatc
     4501 gcatggttga tccgaaatac gacgcctgag ttcttctccg gtcgcaaaga aatgcagagt
     4561 caagccgcgc agttccgttc ccagaaggtt gcgatagtat tcctgaatat tcgagtccgg
     4621 ttcgagaata tatgcctgca tcacaccggc ctgtaaacgt agattcgatt tgcggaatgt
     4681 tcaccagccg gattgaactg gcgacgcact tcaggaggaa gagaatttac agtgcgttct
     4741 gttattgcga ttgtatttgt ttccgcgcag tgcttttcca cttcttccgc gaagcgaatg
     4801 tccggactca ggattttttc cgtgtcactt ctgtagactg cgaggcctgc gtgaagaccg
     4861 atgcgaagcg aaacatcgtt ctcaggtctc agttcgtttt gagtcagatt aaaaatcggc
     4921 agataggcca gaatggcaac agatgaaatg actgctcgcg atgcggcgtc cgcgccataa
     4981 aaggccatca cgcctccatc gccatgccag taccaaatgc gtccgttcct ggcttcaatt
     5041 tgatcctgca agtaggcgcg catacgaacg agagtatttt caacgtccac gggaacattg
     5101 gttctggcaa gagcagaact cccaacaatg tctatggaac caaacacgag cgaatactca
     5161 cttccttcct ggagaaatcc ccagtcagcg gtacgagtct ggctctggct cttgatgaat
     5221 ctgcctgcag atggatcata gatccactga cgttcgcgca atttcttaat gattggatct
     5281 ggattcacaa agtgaatcac gccgccgctt gcgttcttgc cttccatgaa aagaagagtc
     5341 gcaaacagat ggagaaatgc atcttctgtt gcaaaataat ctacaatcac gtctgcggcg
     5401 gtctgtcgcg taacaacgat actgtgacca aagccggaaa cctttccaag atcaaagcgc
     5461 gggtccgctt ccttggcgac gtaattcatc tggtccgtat tcaaagactt ccagagaaac
     5521 tctttgacta gcttacgtat gtctttgaga ttctctgctg ccataaagag ttcgaagaac
     5581 tttgcgaaca cataccgtta tctgaaaagt actttttccc ctgctctcgg gcaggtttgg
     5641 cctgtggcgg gcactcgatt cggcctgatc ttccgtccag gcctggattc atccgggctg
     5701 gtctcagggt atcgtcaggg aaaccgttag gcgatcttga gatgcgggtc tcaaagacca
     5761 gggagagtca gtacggcagg gcctggagtc ctgtgatctg gcagtaatct ggttgaagga
     5821 aagaagccga ctcgactccg aagtcttgca ctcattcgga agtggttggc tgatgaaagt
     5881 gaatgtggag actaccggga tcgaaccggt gaccttctgc gtgcaaggca gacgctctcc
//
LOCUS       DAOQJL010000198         5919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7649_length_5919_cov_3.759209, whole genome shotgun sequence.
ACCESSION   DAOQJL010000198 DAOQJL010000000
VERSION     DAOQJL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_5919_cov_3.759209"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLG78_09895"
     CDS             <1..323
                     /locus_tag="PLG78_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE23470.1"
                     /translation="KPPQFLLTVAPDGATAIESFSKKKFDIVLMDLHLPDMSGYEILS
                     EMRKIENQIGQKHTPVIAVSASVIAEEVTRSLEAGFAHHLGKPISKSGLMAAMHRVLD
                     PELS"
     gene            complement(324..869)
                     /locus_tag="PLG78_09900"
     CDS             complement(324..869)
                     /locus_tag="PLG78_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNE23471.1"
                     /translation="MSDPNFFQTVVLMVEHNAEGAFGLVVNRRSRLVLSDILPGFAHN
                     RGASTPIYVGGPVQQEYLFVLHSDLPDAEAGSEYRSIPVKGVNFEPAFRRIENYFNEE
                     NWNAIPADDRPRIHLYLGYSGWAPGQLEQEMDRGSWIIHPASAKIVFHENPEEGWANA
                     LRDKGGIYRVFADTNPDPGLN"
     gene            complement(924..2525)
                     /locus_tag="PLG78_09905"
     CDS             complement(924..2525)
                     /locus_tag="PLG78_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002744214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNE23472.1"
                     /translation="MDASSTEKPTSPLEREISRRRTFAIIAHPDAGKTTLTEKLLLYG
                     GAIQLAGHVRARRDRRKTRSDWMTMEQERGISISTAALQFEYNGYRMNLLDTPGHEDF
                     SEDTYRTLMAADCAIMLLDASRGVEPQTIKLFKVCRERGIPIITFINKMDLPAKDPFS
                     LLDEVEKVLGITAIPLLWPMGSGKDFRGVYDLVEKKVHRYERVAGGASQVPVTVGGID
                     DPTLDEIMDEYTARVFREGADLVQNALPPFSQEDFIAGTITPVFFGSAVTNFGVQLFL
                     NNFLTLAPQPPPVHLTDGTDLLPSSPVFTGFIFKLQANMNRAHRDRVAFLRITSGKFE
                     RGMTAFVPRLGKQVHLTNPVAFFGQERSTIDDAYPGDIIGMINPGTYHIGDILSTGKP
                     PAFPDLPRFAPDMFASLIPTDTSKLKQFKKGIQELAEEGVVQIFSTQDGSVVIGAAGQ
                     LQFEVFRFRMEDEYGAPCRLEVMPFECSRWIRPSDANKFSSYDRIVKDEKGRTVVLFK
                     SEYRLKSFLQTNADIPVYDHPPQAD"
     gene            2561..3115
                     /locus_tag="PLG78_09910"
     CDS             2561..3115
                     /locus_tag="PLG78_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23473.1"
                     /translation="MKSWEPDQEFERADFPRAGKKPKDAPQKREATPGREVKAWKPRT
                     VSEAAATPRASSSAPAAKSQAPLAKNLDAQGGKSEPRVISSSSGYAAATLGAAILENL
                     EATGAELRHLYWQKEQTGISSKMKIKVIIQKAGAQALGALKSVMTTLERSQMAEIVKT
                     EADCDGATQEQICREFIARIRRAK"
     gene            3112..3672
                     /locus_tag="PLG78_09915"
     CDS             3112..3672
                     /locus_tag="PLG78_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNE23474.1"
                     /translation="MIEFAGYVVLALALTMDTLAVSVVCGNFLKEFRVREALRLGLSM
                     ALVQGAFSLIGVLAGSFAARYLEKVDHWIAFVLLVGIGIKMIRDAYKSDDDEEAPSLA
                     LRVILLMSVATSIDSIMACAGLALTDPHLINAVPVIAVMTLLISLVGTYAGAKLGAAI
                     GSHAGALGGIVLIGLGSRILYLHFAA"
     gene            complement(3682..4677)
                     /gene="galE"
                     /locus_tag="PLG78_09920"
     CDS             complement(3682..4677)
                     /gene="galE"
                     /locus_tag="PLG78_09920"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNE23475.1"
                     /translation="MDVLVVGGCGYIGSHACKILSEKGYNVINLDNLSRGHREFAQWG
                     TLYEGDLGNSDLLDQIFSRHKIEVVMHFAALAYVGESVTKPDIYYQNNVQKPLVLLDA
                     MRKHKVEYFIFSSTCATYGELTPESIPVREDCAQNPVNPYGRSKYFFEQILLDYEKAY
                     GLKSLFFRYFNAAGADPSGAIGEWHEPETHLIPLIFQATLGVVPELTVFGSDYVIPGG
                     NGDGTAVRDYIHINDLVQAHILGIDYLRRINKTEAFNLGNGKGFSVLEMIQTGERITG
                     MKVPYKLGPRRAGDPPVLVGAAEKARNLLGWKPEFPHIERIMETAWNWEKKFRKK"
     gene            complement(4677..>5919)
                     /locus_tag="PLG78_09925"
     CDS             complement(4677..>5919)
                     /locus_tag="PLG78_09925"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE23476.1"
                     /translation="SDTRVNELWLELARQDFEMVDRLTRIFYKNKFFFYPLKPFNALF
                     NLGPIESVLCFLSYLKEKVKPTPDDGSFETWVTGRFGKKLFSIFFKTYSEKLWGISCK
                     DLDAEFAAQRIKKLSLWEAVKNALFGGRGNKHKTLVDQFAYPSGGTGMIYERMADFIR
                     SKGSNVMLKTPVQRVITENGKATAIETEGGRTEKYDHVVSSMPITLLVSRLPEVPEPI
                     KEFSKKLKFRNTVLVYVEVKAKDLFPDNWLYVHSDNLLMGRLTNFRNWVPELYGKEES
                     TIMVLEYWCYDEDAFWTRTDEDLIELAKKELKSTGLIGNAEMPRGHVHRIHRCYPVYD
                     RGYKERLKPVEDYLSSVGNLSVIGRYGAFKYNNQDHSILMGILASENILHGKNNNLWD
                     INTDYEYQESSKITKTGLVKG"
BASE COUNT         1364 a         1447 c         1523 g         1585 t
ORIGIN      
        1 taaagcctcc gcagttttta ctcacagtgg cgcctgacgg agccacggcc attgagtctt
       61 tctcaaaaaa gaaatttgat atcgtgctga tggatctcca tcttccagat atgagtggct
      121 acgaaattct atctgaaatg cgaaagatcg aaaaccaaat tggtcagaag catacaccgg
      181 tgatcgcagt atccgcgagc gtcatcgcag aagaggtgac gcggtcactc gaagcaggtt
      241 tcgcacatca tctgggaaaa ccaatttcaa agtcggggct catggcggcc atgcacaggg
      301 ttctggatcc ggaactctcg tgatcagttg agtccaggat ccgggtttgt gtctgcgaat
      361 acgcggtaga tgccgccttt gtcacgtaga gcgttggccc agccttcctc cggattctcg
      421 tgaaagacga tcttcgcaga tgcagggtga atgatccagc ttccgcgatc catttcctgt
      481 tccagctggc ctggcgccca gccagaatat ccaaggtata aatgaattct cgggcgatca
      541 tctgctggaa ttgcattcca gttttcttcg ttgaagtagt tttcgattcg tctgaatgcg
      601 ggttcgaaat tgactccttt gactggaatg ctgcgatact ctgagcctgc ctccgcgtcc
      661 ggaagatccg aatgcaatac aaaaagatat tcctgttgca ccgggccgcc gacgtagatg
      721 ggagtgctgg ccccgcggtt atgcgcgaaa ccaggaagga tgtcagatag aacgagtcgt
      781 gatcttctat ttaccacgag gccaaatgcg ccttccgcat tgtgttccac catgagaacg
      841 actgtctgaa aaaaattggg atcagacatg ttcgcttccg aaatcaggaa atgtcctttc
      901 agtgaatcgg ggcgtttagt cattcaatcc gcctgaggag ggtgatcgta aaccggaata
      961 tcagcatttg tctgtagaaa actctttaga cgatattcgc tcttaaatag cacaactgtt
     1021 cgcccttttt catcttttac aatcctgtca taagagctga atttgttcgc gtcagaagga
     1081 cgaatccacc tggagcattc aaacggcatc acctcgagtc tgcagggcgc accatattca
     1141 tcttccatgc gaaaacgaaa cacttcaaac tgaagctggc ccgcagcccc gatgaccaca
     1201 ctgccatcct gtgttgaaaa tatttgaacg accccttcct ctgccagttc ctgaattcct
     1261 ttcttgaact gtttgagttt ggatgtgtcc gttggaatga ggcttgcgaa catatccggc
     1321 gcgaatctgg gaagatctgg aaatgcgggc ggcttaccgg tggatagaat gtccccaatg
     1381 tgataggtgc ccggattgat catcccaatg atgtcgcctg gataggcgtc gtcaatcgta
     1441 gagcgttctt gcccgaagaa tgcgacgggg tttgtaaggt gaacctgctt gccgagtcgc
     1501 ggaacaaacg cagtcatccc ccgttcgaat ttcccgcttg tgatccgaag gaatgcgaca
     1561 cggtcgcgat gagcgcgatt catgtttgcc tgaagcttga aaatgaatcc ggtgaaaacc
     1621 gggctcgatg gaagtagatc tgttccgtct gtgagatgca caggcggtgg ttgtggggca
     1681 agagtcagga agttgttcag aaacaactgc accccgaagt ttgtaacagc agaaccaaag
     1741 aacacaggag taattgtacc tgcgatgaaa tcttcctgac tgaagggcgg cagtgcgttc
     1801 tgcaccagat ctgcaccttc gcggaagacg cgggcggtat attcgtccat aatctcatcg
     1861 agagtcgggt catcaatgcc gcctaccgtt accggaacct gggaagcgcc acctgctact
     1921 ctttcgtagc gatgtacctt cttttccacc agatcgtaga ctccgcgaaa gtcttttcct
     1981 gaacccatgg gccatagaag cggaatcgcg gtgattccca ggaccttttc cacttcgtcc
     2041 aggagagaga atggatcttt ggcgggaagg tccatcttgt tgatgaaggt gatgatcgga
     2101 attcctcttt cgcggcatac cttgaagagc ttgatcgtct gtggctctac accgcgagaa
     2161 gcatccagaa gcatgatagc gcagtccgct gccatcagcg tgcgatacgt atcctcggaa
     2221 aaatcttcat ggcccggtgt atccaggaga ttcattctgt aaccgttgta ttcaaattga
     2281 agcgcggccg tgctgatgga gattccacgc tcttgttcca tggtcatcca gtccgatctc
     2341 gttttgcgtc tgtcccttct tgcgcgtaca tgacccgcca gctggatggc tcctccgtac
     2401 agcagcaatt tttccgttag cgtggtcttc cctgcatcgg ggtgagcgat gatagcgaac
     2461 gtgcgacgac gcgaaatctc tctttccaga ggtgatgttg gtttttcagt ggacgaagcg
     2521 tccatgcggg acacgatttc tggaaaagcg gagcgcacaa atgaaatcct gggaaccgga
     2581 tcaggagttt gaacgggcgg attttccccg tgcgggcaag aagccaaaag acgcgcctca
     2641 gaaacgcgag gcgacgccgg gccgcgaagt aaaagcctgg aagcctcgca ctgtttctga
     2701 ggcggccgct acaccccgcg cctcgtccag cgctccggca gcgaaatctc aggcacctct
     2761 tgcaaagaat ctggacgcgc aaggcggcaa atcagagccg cgcgtgattt ctagttcctc
     2821 cggatatgct gcggccaccc tgggtgctgc tattctggaa aatctggagg ctacgggggc
     2881 ggagctcaga catctgtact ggcagaaaga gcagacgggc atcagttcta agatgaagat
     2941 caaggtgatc attcagaagg cgggtgctca ggcgctcggc gcattgaaat ctgtcatgac
     3001 tactctggag cgctctcaga tggcagagat agtaaaaaca gaagcagatt gcgatggagc
     3061 cacgcaggaa cagatttgca gggaattcat cgcgcggatc aggcgggcaa aatgatagaa
     3121 ttcgcaggtt acgtggttct cgctctcgct cttacaatgg atactctggc ggtctccgtg
     3181 gtttgtggga attttctaaa agagtttcgc gtacgcgaag cgcttcgtct tggtttgagc
     3241 atggctctgg tccagggggc cttttctctc attggcgttc tggccggatc tttcgctgcc
     3301 cgctatctag aaaaagtgga tcactggatc gcctttgtgc tgttagtcgg aattggcatt
     3361 aagatgattc gagatgccta taaatctgat gatgacgaag aggcaccatc tctggctttg
     3421 cgagtcattt tgctcatgtc tgtggcaacg agcattgatt cgatcatggc ttgcgcagga
     3481 cttgccctca cggatcctca tctaatcaac gcagttcccg tcatcgcagt tatgacactg
     3541 ttgatttcac tggttggcac gtatgcggga gcaaaactgg gagcggcgat tggttctcac
     3601 gccggggcac tcggtggcat tgtgctcatt ggactcggtt ccagaattct ctatttgcat
     3661 ttcgcagcct agctgcggcg cttatttctt tctgaatttc ttttcccagt tccaggcagt
     3721 ttccataatt cgctcaatgt gagggaattc tggtttccat cccagtagat ttcgcgcctt
     3781 ttctgccgca cccaccagaa caggaggatc tccggctctt cttgggccga gtttgtaggg
     3841 aactttcatt cctgtgattc tctcgcctgt ctgaatcatt tccagtacag agaacccttt
     3901 accatttccc aggttgaagg cttctgtttt gttgattctt cttaaataat cgattccgag
     3961 tatgtgagcc tgtacgagat cgtttatgtg aatgtaatcg cgaactgcgg ttccgtctcc
     4021 attgccgccc ggaatgacgt aatcagagcc aaatacggtg agttcgggca ctacacccag
     4081 agttgcctgg aaaatcagag ggatcaggtg cgtctccggt tcgtgccatt caccaatggc
     4141 gcctgacggg tccgcgcctg cagcattgaa ataccgaaaa aacaaggatt tcagtccgta
     4201 tgctttttcg tagtccagga gaatctgttc aaagaagtat ttagatctgc cgtaaggatt
     4261 caccggattc tgcgcgcaat cctctcgcac tggaatgctt tccggagtga gttctccata
     4321 cgttgcgcac gtggaagaga aaatgaaata ttctactttg tgctttcgca tcgcatcaag
     4381 caagacgagc ggcttttgca cattattctg gtagtaaata tccggttttg tgactgattc
     4441 tcccacgtaa gccagtgctg caaaatgcat cacgacttca attttgtgtc gcgaaaaaat
     4501 ctgatcgagt agatccgaat tcccgagatc tccctcgtac agagtgcccc attgcgcgaa
     4561 ttctcgatga ccccggctta gattgtcgag gttgattacg ttgtagcctt tttccgaaag
     4621 aatcttgcat gcatgcgagc ctatgtagcc gcatccgcct acaaccagaa cgtccactat
     4681 ccttttacca gccctgtttt ggtgattttg gaggattcct ggtattcgta gtcagtgttg
     4741 atatcccaca gattgttgtt tttgccgtga agaatgtttt cagaggccag gattcccata
     4801 agaatagaat gatcctggtt gttgtacttg aacgcgccat atcttccgat gacgctgagg
     4861 ttcccaacac tcgagagata gtcttccact ggcttaagtc tttctttgta accacggtca
     4921 tagacaggat agcagcgatg aatgcgatga acatgcccgc gaggcatttc cgcgttgccg
     4981 atgagcccgg tggatttgag ttctttttta gcgagctcga ttaagtcttc atctgtgcga
     5041 gtccagaatg cgtcttcgtc atagcaccag tattcgagaa ccatgatcgt ggattcttct
     5101 ttgccgtaga gttctggtac ccagttgcga aagttcgtca gtcgtcccat gagtagattg
     5161 tctgaatgca catagagcca gttgtccgga aatagatctt ttgctttcac ttccacgtaa
     5221 acgagcactg tgtttctgaa ttttagtttt ttcgaaaatt ctttgatggg ctctggaact
     5281 tctggaagtc tgctcacgag cagagtgatg ggcatagagg aaactacatg atcatatttc
     5341 tctgtgcgtc cgccttctgt ttcaatggca gttgcttttc cgttttctgt gatgactctc
     5401 tgcacaggag tcttgagcat tacgttactg cctttgcttc gaatgaaatc tgccatgcgt
     5461 tcgtagatca ttccagtgcc gcctgacggg tatgcaaact ggtccacgag agttttatgt
     5521 ttgtttccgc gacctccgaa gagagcgttc ttcactgctt cccacagaga gagtttctta
     5581 attctttgcg cggcgaactc tgcatccagg tctttgcagc tgatgcccca gagcttttcg
     5641 ctgtaggtct tgaagaaaat agaaaacaac tttttgccga atcttccggt cacccaggtt
     5701 tcaaaagatc cgtcatccgg cgttggtttt actttttctt tcagatagct gagaaagcac
     5761 agtacagatt caatgggccc gagattgaac agcgcgttga atggtttaag aggatagaag
     5821 aagaacttgt ttttgtaaaa gatgcgcgtg aggcgatcca ccatttcaaa gtcctggcga
     5881 gccagttcca gccacagttc attgacgcgt gtgtcgctg
//
LOCUS       DAOQJL010000199         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7701_length_5892_cov_4.602707, whole genome shotgun sequence.
ACCESSION   DAOQJL010000199 DAOQJL010000000
VERSION     DAOQJL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_5892_cov_4.602707"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PLG78_09930"
     CDS             complement(<1..1724)
                     /locus_tag="PLG78_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNE23477.1"
                     /translation="MKMEQDRIYLRSPQIRNLAVLFLFAISVFAVRAEEKFDPSSVQP
                     ETCGPPLYACGVAQPDLETMRAIPVYRGIPRGLPSSADISSRMPPPSSQGRQGSCVSW
                     ATAYATRSFFEQAARNWGYDAPVTGGRGEHIFSPAYIYNQVNGGRDQGSTIPESLQLV
                     MEQGVAPWSAMPYSDRDYRTKPNAAQRKAASRFRIGSYAFLARHSVDGYKAEIAAGRP
                     IPFAMTVYQNFYNVSDKVYDSAEGRILGGHAMVIVGYDDDKVSPKGDRGAFKIMNSWS
                     KHWGQGGFGWISYKRWLALTSGGYAITPVDLEPEPLPANQISPPVSVTATQGAYSDRV
                     VVYWAAVRDARAFTIYRAEPGASFEEIGNSGSHSFTDRAVKMNVAYRYRVSAVRDAKV
                     FSDPARSPIAEGYAAMNPSGNPQKVTGLMADAISIGRNPAVRLSWTPTPGASSYEVSR
                     YDSLSRKWKTIQRPMEPQITDSAVAPDATVSYSVRAIGSTEGPWSDAVQLKIAGEVTV
                     PSVPSNVRAVANNEGIMVISWDAVPGAESYNVIRYSHRTGKWTRPASVSDTQYVDKDS
                     TRSAEDFL"
     gene            1880..2212
                     /locus_tag="PLG78_09935"
     CDS             1880..2212
                     /locus_tag="PLG78_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE23478.1"
                     /translation="MITKAAEFPATLNELADFAKALSHPARTQILKTVAKRGECVCGE
                     IVDVMPLAQSTVSQHLKDLKGIGLIKGETEGKTSCYCIDWKAMKRFKSLASQFFEEME
                     KFEKSNCC"
     gene            2229..3092
                     /gene="arsM"
                     /locus_tag="PLG78_09940"
     CDS             2229..3092
                     /gene="arsM"
                     /locus_tag="PLG78_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNE23479.1"
                     /translation="MKTSEEIKSMVRETYAGIAQAGPQPSGQSCCGTQKGNVWFNEDY
                     SKLEGYVADADLGLGCGIPVEFSKIKNGDVVIDLGSGAGNDAFIARRQTGETGKVIGI
                     DMTSEMIDRARENNDKLGYNNVEFRLGEIEKLPVSANQADVIISNCVLNLVPDKQAAF
                     LEIFRTLKPGGHFCVSDIVLSGPIPEKLRSVASMYAGCVSGAVQKEEYLNQIKSAGFD
                     NISINKERTIHIPQEVLAEHLTKEEMETLASAKLEVLSITVSGEKPSLKFLQTEKAAA
                     KEACCAPGCCA"
     gene            3080..3544
                     /locus_tag="PLG78_09945"
     CDS             3080..3544
                     /locus_tag="PLG78_09945"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNE23480.1"
                     /translation="MLRITMVKPRILILCTGNSCRSHLAEGILRQAAGDFLEVASAGS
                     KPAGFVHPLAIRAMKEIGIDISEHTSKSMNDFLGSQIETVITVCGNADQACPVFPGQV
                     NRHHWPFDDPAHATGTEEEQMEVFRRVRDEIKKVFEAYADGRRDAIRTGTSK"
     gene            3541..4632
                     /gene="arsB"
                     /locus_tag="PLG78_09950"
     CDS             3541..4632
                     /gene="arsB"
                     /locus_tag="PLG78_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNE23481.1"
                     /translation="MKQEIRHLDFFERYLTIWVLGCMAIGVTLGMLFPETSRTLGALE
                     FTQGSHINAPIAVLIWLMIYPMMLKVDFGGLREVVRKPKGLAITLVVNWLIKPFSMAL
                     LGWIFLHLFFRNLGLMTEEAARDYNAGLIILAAAPCTAMVFVWSYLTNGDSAYTLAQV
                     AVNDLIMLFLFAPIVMLLVGVTGVVVPAQVLIASVVTFIVLPLIAAFASRVFLIRAKG
                     KEWFEQTFLQALKPVTIGALLATLALIFMFQSRNIRENWLAVVLIAVPITIQVYFNAG
                     ITYWLMKIFRVPHNVAAPGSLIGASNFFELAVAVAITLFGTQSGAALATIVGVLVEVP
                     VMLSVCRICVGSRSWYQANTGSPATSVDG"
     gene            complement(4618..>5892)
                     /locus_tag="PLG78_09955"
     CDS             complement(4618..>5892)
                     /locus_tag="PLG78_09955"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE23482.1"
                     /translation="LSTSMSRAGVTPLAPAPPQNAALVTLGRALFFDKELSGNRNIAC
                     STCHLRNSGTSDGLSLSIGWGGNGLGAVRTVGTGNFVGRNAPPLFNLGAKITKYFWDG
                     RVSRADNGILTTPEPALNGASPTRADIASQLTTGLAAQAMFPVTSDLEMRGLPGNPVR
                     DAANNQAVWAVLMSRLVGTNNGTVGGLPGYRALFAAAFPTVTNFDNFNFGHAAAAIAA
                     FETESYSAVGSPFEQYVKGNTGALTPEAKAGGILFFGKANCSTCHNGPMLTDNEFHGV
                     ASPQILGTSGYLNTGDDTGRNVVTGQPGDMYKFRTPNLHNVELSAPFTHSGAYASLNQ
                     VVDHYTNPSNAQLNFSAQGLRPDFAFTADLNFAHKTNRINALDPILRQPLVLSAQEKQ
                     SLVAFLMSLTDPQARNLTGSAPASVPSGLSID"
BASE COUNT         1484 a         1526 c         1501 g         1381 t
ORIGIN      
        1 taaaggaaat cctccgcgct acgtgtcgaa tctttgtcca catactgtgt gtcagatacg
       61 gacgcaggcc tggtccactt tccggtgcga tgcgaatagc gaataacgtt ataagattcc
      121 gcccctggaa ctgcatccca ggaaatcacc atgatacctt cattgtttgc tacagcacgg
      181 acattggaag gaacagaagg gacagtcact tcgcctgcga tcttcaattg cacggcatcg
      241 ctccagggac cctcggtcga tccgatggct cgcacactgt aggaaaccgt agcatctgga
      301 gccactgcag aatcagtgat ctgcggttcc attggccttt gaattgtttt ccatttacgc
      361 gaaagagagt catacctgga cacttcgtaa gaagacgccc ctggagtggg agtccaagaa
      421 agcctcactg ccggatttct gccgatcgaa attgcgtccg ccatcagacc ggttaccttt
      481 tgcggattgc ctgaaggatt catagctgcg tatccctcag caatcggcga tcgagcagga
      541 tcagagaaaa ccttcgcatc cctgaccgcc gaaacgcgat accgataggc gacattcatt
      601 ttgactgctc tatccgtgaa tgaatgcgag ccggaattgc caatctcttc aaaagaagct
      661 cctggctctg cccgataaat tgtgaacgcc cttgcatcgc gaactgcggc ccagtagacg
      721 accacgcgat cagaatacgc accctgtgtt gcagttacac tcactggagg agaaatctga
      781 ttggcgggaa gcggttcggg ttcaaggtcc acaggtgtga tcgcataacc gcctgaagtt
      841 aatgccagcc atcttttgta ggagatccac ccaaaaccac cctgccccca gtgcttgctc
      901 catgagttca tgattttgaa ggcaccgcga tctcctttcg gagaaacttt gtcatcgtca
      961 taaccaacaa tgaccatcgc gtgaccgccg aggatccttc cctccgctga atcgtacact
     1021 ttgtccgaga cattgtaaaa attctgatat actgtcatgg cgaaaggtat gggcctgcct
     1081 gcggcgatct ctgccttgta gccatccacg gaatgccgag cgagaaacgc atagcttccg
     1141 atcctaaaac gcgaagccgc ttttctctga gctgcattcg gtttggttcg atagtctcta
     1201 tccgaatatg gcatcgcact ccagggtgcc acgccctgct ccatcaccag ctgcaaggat
     1261 tctggaatcg tgctgccctg atcccttccg ccattcactt gattgtaaat gtacgcaggc
     1321 gaaaatatat gctcacccct gccgcctgta acgggcgcat cataacccca gttgcgcgcg
     1381 gcctgttcaa agaaagaacg agtagcgtaa gctgtggccc aggaaacgca gcttccctgt
     1441 ctgccctggc tcgacggcgg cggcatgcgc gaggaaatgt ccgcagaaga cggcagccca
     1501 cgtggaatcc cgcgatatac tggaatcgcc cgcatggttt ccaggtccgg ttgcgccaca
     1561 ccacatgcat agagcggcgg gccgcacgtt tccggctgca cagaagaagg atcgaacttt
     1621 tcttccgccc tgacagcaaa aacagaaatg gcaaacagaa acaggacggc cagattgcga
     1681 atctgcggcg acctgaggta gattcgatct tgctccatct tcatgcggaa catgtaaact
     1741 ccgacgcatg aaatctccgc aagaagaaaa gggttgtttc agccaattct gatcggccac
     1801 ctctgcaccg ttattcgccc gtaaacggta cagaattttt ttcttgccgg gtattccact
     1861 gatcgtataa ttacgatata tgataacaaa agcggctgag ttcccggcta cattgaacga
     1921 gcttgcggat ttcgcaaaag cactatcaca tccggccaga acacagatac tgaaaaccgt
     1981 tgcaaaacgt ggagaatgcg tctgcggcga aatcgtggat gtaatgcctc tagctcagtc
     2041 cacggtatcc cagcatctca aggacctgaa gggaattggc ctcataaaag gcgaaacgga
     2101 gggcaagacg tcgtgctact gcattgattg gaaggccatg aaacgattca agtccctcgc
     2161 ctcgcaattc ttcgaagaaa tggaaaagtt cgaaaaatca aactgctgct aatgaaagga
     2221 gaagcaaaat gaaaacgtca gaagaaatca aaagcatggt tcgcgaaacc tacgcgggaa
     2281 ttgcgcaggc aggtccacaa ccatccggtc aatcatgttg cggcactcaa aagggaaatg
     2341 tctggttcaa tgaagactat tccaaactgg aaggttacgt tgcagacgca gacctgggtt
     2401 taggttgcgg aatccctgtg gaattttcga agatcaagaa cggcgacgta gtcatcgatc
     2461 taggatccgg cgcaggcaat gacgcgttca tcgccaggcg ccagacagga gagacgggta
     2521 aagtcattgg aatcgacatg acgtctgaaa tgattgatag agcgcgagaa aacaacgaca
     2581 aactcggata caacaatgta gagtttcgtc ttggagaaat tgaaaaattg cccgtcagcg
     2641 cgaatcaggc ggatgtgatc atcagcaatt gtgtgctcaa tctggttccg gacaaacaag
     2701 cggccttcct ggaaatcttt cgcacactga aaccaggtgg ccacttctgt gtgtctgaca
     2761 ttgttctttc ggggcctatc ccggagaaat tgcgttctgt agcttcgatg tatgctggat
     2821 gtgtatccgg tgctgttcag aaagaagaat acctgaatca gatcaagagc gccggatttg
     2881 acaatatctc gatcaacaaa gaacgaacca tccacattcc tcaggaagtt ctcgcagagc
     2941 atcttacaaa agaagagatg gaaaccctgg cgtccgctaa actggaagtt ctcagcatta
     3001 cagtcagcgg tgaaaagcct tctctcaaat tcctgcaaac agaaaaagcg gcagccaaag
     3061 aagcatgctg cgcccctgga tgctgcgcat aacaatggtt aaaccgcgca ttctgattct
     3121 ttgcaccgga aattcctgcc gcagtcatct cgcagaaggg atcctgcgcc aggcagccgg
     3181 tgactttctt gaagtggcca gtgccggttc aaaaccggcc ggctttgttc atccacttgc
     3241 cattcgcgcc atgaaagaaa tcggcattga tatttcagaa cacacttcga aatccatgaa
     3301 tgattttctc ggttcacaaa ttgaaacagt cattactgta tgcggaaatg cagaccaggc
     3361 atgtcctgtt tttccaggcc aggtgaaccg tcatcactgg cctttcgatg atcctgctca
     3421 tgccactgga accgaagaag agcagatgga agtgtttcga cgcgtgcgag acgaaatcaa
     3481 gaaagtcttc gaggcatacg cagatggcag acgagatgcc atcagaaccg ggacatccaa
     3541 atgaaacaag aaatcaggca tctagatttt ttcgaacgtt atctgaccat ctgggtactt
     3601 ggatgcatgg caatcggcgt aactcttgga atgctctttc cggaaacgtc gcgaactctg
     3661 ggcgcgcttg agttcacgca gggatcgcac atcaatgcac cgatcgccgt gctcatctgg
     3721 ctgatgatct atcccatgat gcttaaggta gattttggtg gtctacgcga agtggttcgc
     3781 aagccaaaag ggcttgccat cactctcgta gtcaactggc tcatcaaacc attcagcatg
     3841 gctctacttg gctggatttt cttacatctg tttttcagga atcttggtct catgacggaa
     3901 gaagcggctc gagactacaa tgcaggcttg atcatcctcg cggccgcacc gtgcactgcg
     3961 atggtctttg tctggagcta tcttacaaat ggagactccg catacactct tgcacaagtt
     4021 gcagtgaacg atctcatcat gctgtttctt ttcgcgccca ttgtcatgct tctcgtggga
     4081 gttacagggg ttgtggtgcc agcccaggtt ttgattgcat ccgtggtcac tttcattgta
     4141 ctccccctga tcgcggcgtt tgccagccga gtcttcctga tccgtgcgaa aggaaaagaa
     4201 tggtttgagc aaacatttct acaggctctc aagccagtga ccattggcgc acttcttgcc
     4261 acgcttgcct tgatcttcat gtttcaatca cgcaacatac gcgagaactg gctggctgta
     4321 gtgctgatcg ccgtgccgat cacgattcag gtctatttca atgcaggcat aacttactgg
     4381 ctgatgaaga tcttcagagt gccgcataac gttgccgcac cgggttctct cattggcgca
     4441 agcaacttct ttgagctggc agtggcagtg gccatcacgc tttttggaac acaatcaggc
     4501 gccgctctgg caaccattgt gggtgtgctc gtggaagtac cggttatgct ttccgtgtgc
     4561 agaatctgcg ttggctccag gtcgtggtat caggcgaaca ctggctcgcc cgcaacgtca
     4621 gtcgatggat aatccgctgg gcacgcttgc cggcgcactg cctgtcaaat tgcgtgcctg
     4681 aggatccgtc agagacatca gaaaagcgac cagactctgc ttttcttgcg ccgaaagaac
     4741 cagaggctgt ctcaagattg gatccagcgc attgattcga tttgttttgt gtgcgaaatt
     4801 cagatcagcc gtgaatgcaa aatccggtct aaggccctgt gcagaaaagt tcagttgagc
     4861 gttagatgga ttcgtatagt gatccacaac ctgattgaga gaagcatatg ccccagaatg
     4921 agtgaatgga gcagacagtt ctacattgtg aagattgggc gtgcgaaatt tgtacatgtc
     4981 gccgggctgc cctgtaacta catttcgccc ggtgtcgtct ccggtgttta gatagccgga
     5041 cgtacccaga atctgcggag aggcaactcc gtgaaactca ttgtccgtca gcatgggtcc
     5101 attgtgacac gtggagcagt ttgcttttcc gaaaaacaaa atcccgcctg cttttgcctc
     5161 tggtgtgaga gcgccagtat ttcctttcac gtattgctcg aagggtgagc ccactgcaga
     5221 ataactctcc gtttcaaagg cagcaattgc tgctgcggca tgtccgaagt tgaaattatc
     5281 gaagttggta acagtaggaa aagcggcagc aaaaagagcg cgataaccgg gcagtccacc
     5341 tacggtaccg ttattcgttc caacgaggcg agacatgaga acagcccaga ccgcctgatt
     5401 gttggccgca tctctgaccg ggtttcctgg aagacctcgc atctcaagat cggacgtaac
     5461 tggaaacatt gcctgtgcgg ccagaccggt cgtgagttga cttgcaatgt ctgcgcgcgt
     5521 cggagatgca ccgttgaggg ctggctcggg tgtggtaagt ataccgttat ccgctctact
     5581 cacacgccca tcccagaagt atttcgtaat cttcgcaccc aggttaaaca aaggcggagc
     5641 atttcttccg acaaagtttc ctgttccaac ggttcgcact gcaccaagtc cgtttccacc
     5701 ccatccgatc gacaaggaga gaccgtctga tgtgcccgag ttgcgaagat gacaggtaga
     5761 gcaagcgatg ttgcgatttc ctgaaagttc tttatcaaag aaaagagcgc ggcccagagt
     5821 cacaagagcc gcgttctgag ggggcgcggg cgccagagga gtcacacctg ccctgctcat
     5881 gctagtgctc aa
//
LOCUS       DAOQJL010000200         5863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7760_length_5863_cov_4.037534, whole genome shotgun sequence.
ACCESSION   DAOQJL010000200 DAOQJL010000000
VERSION     DAOQJL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7760_length_5863_cov_4.037534"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLG78_09960"
     CDS             <1..429
                     /locus_tag="PLG78_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23483.1"
                     /translation="NGGKSTLASYRLSPGASFDSVPETHRAFLDSLLLYYESDNFLCV
                     HAGLDVSEPDISRQMRDTILWIRTPWIQHESKWKGKHVYYGHTPTRYVNGMENYRELI
                     DGRKSTGLDTGCVYGGCLTALNAENGAVIQVAAEKNYWDP"
     gene            426..1685
                     /locus_tag="PLG78_09965"
     CDS             426..1685
                     /locus_tag="PLG78_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-A polymerase"
                     /protein_id="HNE23484.1"
                     /translation="MKLIEKLDDQTREDIRTISDILSQSSYQCYMVGGCVRDLLLGRP
                     VKDIDITTNAEPQVVQKLFKRTVPTGIQHGTITVLFEKRSYEITTYRAEGTYSDARRP
                     DTITFAKTLSEDLSRRDFTINALAYDPTADVLQDEHGGLDDLKQKIIRTIGKAHDRFF
                     EDGLRTVRACRFASVLDFRIEEETFKAICDPAIHERTKKVAVERFSDELRKGLSGPFP
                     SRLIELLEDTGLMNLFLPYKAPRPELQKLSLLSNVENRLGCYLHFICMREKDPVASIE
                     STAKKLKLSNETAAQAIRTHRFLLASAGDLNDVEEQIDQRRFCAWLKKTSADPLSILD
                     DSSELASIQSSLQTLIPVWKTMIQNDPLVAKDLALNGNDIQELGFSGKEIGTAQASAL
                     EYLYRWPGRNTREDLTQFLLGKRSSSS"
     gene            complement(1649..2326)
                     /locus_tag="PLG78_09970"
     CDS             complement(1649..2326)
                     /locus_tag="PLG78_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE23485.1"
                     /translation="MSGLRDCKAILFDVDGTIFSSEEIIHEIYIEEFEKTRALLGRPA
                     HIPTRPEIIEQIGKPVPVIFANLAPDLSRPEQDELSGRILEGLVTGIAAGRGHHYPGV
                     TETVRELKKRGYDLFTASNGRAPYVRSILQAGGIFPEFKSIPTVGGTIQNKSELVASI
                     LKENLLMPAQAALIGDRASDRDAAIENQVPFVAARFGHGTEEEWGGSVARIDSIHELL
                     DLFPSRN"
     gene            complement(2323..3165)
                     /locus_tag="PLG78_09975"
     CDS             complement(2323..3165)
                     /locus_tag="PLG78_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23486.1"
                     /translation="MKLAAMVIALSVAACSPQLRSAEAPPNFPVEGLWIQDTAKAGED
                     HFTILWVEKSASKTPGAAPYTFSFVRTVARNLAWNDQTLFVTRRTGDLRTSGKEVLLS
                     QQVYLSSKRSVLENVKGKQIWPANQFGPEEDQRKIEDGYNLDLLELSEDGNTLSGKTG
                     TFYRGNPSGFGRFPQAVVCKVDKDNKEVALYGLPTGKFATGQVLKNAKGDIALRATGQ
                     NAEYVSATIEKGLPVIGDVVLPSDAKEDPKMKPLSKEEVLERIRRGEFVPRDDLIRVL
                     GDKK"
     gene            3195..4160
                     /locus_tag="PLG78_09980"
     CDS             3195..4160
                     /locus_tag="PLG78_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE23487.1"
                     /translation="MNTKKTSARRPGLEHAPVVLTDYRIEAGTFSGAGGLTLRYKAWI
                     CEDVDSGRNLVFHHGFGEHCGRYEYLVEALAGDRVNLFSFDARGHGRSEGIRGGAGDI
                     TDMVRDLEHFMTFLREEFGVRCPLLLGHSMGGLVVLCFALHFSNQWETRGLLTSGAAL
                     KVKMNPVLRIKQVSGQIIGQFAPDLVLPAGVDPNLLSHDPREAQAYEDDPLVHGNISI
                     RLGLDMVRQGKLAIENAARIKVPIFMGHGGGDEVADPLGTRQFFKGVQSEIAELRIYP
                     GLYHEIFNESPDARSAVFDDYRRFIQNILAATETILPPVEPATES"
     gene            4246..4623
                     /locus_tag="PLG78_09985"
     CDS             4246..4623
                     /locus_tag="PLG78_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23488.1"
                     /translation="MEKILQDILNAGIALFRAGEGTVQNAVKEVQRTFEQLKEKGAQD
                     NSEQAVKLRKTLDDIVHQTSGLSSKAGAAYSDALAQIEQLYKNVGDQIQKLVPPERIN
                     EVKDKIEELTRVIQQRAGGAPKV"
     gene            4664..5047
                     /gene="queF"
                     /locus_tag="PLG78_09990"
     CDS             4664..5047
                     /gene="queF"
                     /locus_tag="PLG78_09990"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNE23489.1"
                     /translation="MDYESKQDHIRELKTPDIEVFRNIYNDREYRIDMEIPEFTAVCP
                     KTGLPDFGILYLSYKPAEHCLELKSLKEYVLSYRNVGIFHENVVNRILDDIVKACSPR
                     WARLHGDYNVRGGIKTRVTAVYRRP"
     gene            complement(5010..5690)
                     /locus_tag="PLG78_09995"
     CDS             complement(5010..5690)
                     /locus_tag="PLG78_09995"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNE23490.1"
                     /translation="MSYDSILDTIPAVEENGRIDLPYRSEIELVLGKSSSAQRCRLVH
                     ISSTSVYAEDFHGEEKTLPCFDEFTPARPETVRGERRLALEERIKESFPDAVILRAGG
                     IYGPGRALPLQFLAGQFGRADSGNRMVSRIHVHDLAQLALAAATSDIDLINAVDGNSA
                     LNSEVFAYLENLLSVKVPGSWATDLPSGRRIISRYADQLIRFRYPDYRSGFEEILRDA
                     DKLPLRES"
BASE COUNT         1507 a         1559 c         1411 g         1386 t
ORIGIN      
        1 aacggaggca aatccactct ggcttcctac aggctttcgc ccggcgcttc gtttgactct
       61 gttcctgaaa ctcacagggc ctttctggat tcacttttac tctattacga atctgacaat
      121 tttctgtgcg tccacgcagg tttagacgtt tccgaaccag acatcagccg ccagatgcgc
      181 gatacgattc tctggattcg cactccctgg attcagcacg aaagcaaatg gaaaggaaaa
      241 catgtgtact acggacacac acccacgcgt tacgtgaatg ggatggaaaa ctacagagaa
      301 ctcatagacg gaaggaaatc cactggtctg gataccggct gcgtgtatgg aggttgcctg
      361 actgctttga atgcggaaaa tggtgcggtc attcaagtgg cagcggaaaa gaattactgg
      421 gatccatgaa actcatagaa aaactagacg atcaaacccg cgaagacatc cgcaccatca
      481 gcgacattct ctcacaatcg tcttaccagt gttatatggt aggcggctgc gtgcgggact
      541 tactcctcgg tcgtccggtc aaggacattg acataactac aaatgcagaa ccgcaagtag
      601 ttcaaaaatt gtttaaaaga acagtgccca ctggcattca gcacggaacc atcactgtgc
      661 tttttgaaaa acgttcttac gaaatcacca cctacagagc ggaaggcaca tactcggacg
      721 ctcgcagacc agatacaatc acttttgcaa aaactctctc agaagatctt tcgagacgcg
      781 attttaccat aaacgctctt gcctacgatc caaccgcaga tgtattgcaa gacgaacatg
      841 gcgggctcga cgatctgaaa caaaaaatca ttcgcacgat cggaaaggcg cacgacagat
      901 tctttgagga cggtcttcgc accgtgcgcg cctgtcgatt tgccagtgtg ttggatttta
      961 gaatcgaaga ggaaacattc aaagcaatct gtgatcccgc gattcatgaa cgaacaaaaa
     1021 aagtggccgt agagagattc tcggatgaac tcaggaaggg cctctcaggg cctttcccgt
     1081 ccaggctcat tgagctactc gaagacaccg ggctcatgaa tctgtttctt ccatacaaag
     1141 caccgcggcc ggaattgcag aagctttccc ttctttcaaa tgtagaaaac aggctgggtt
     1201 gttatttgca tttcatctgc atgcgagaaa aagacccggt ggcctcgatc gaatcgactg
     1261 caaagaaact caagctatcc aatgaaactg cggcgcaggc aatcagaacg caccggttct
     1321 tgctcgccag tgcgggcgat ctaaatgacg tagaagagca gatcgatcaa aggaggttct
     1381 gtgcctggtt aaagaaaaca tccgcagatc ctctttccat cctcgatgat tcgagcgaac
     1441 tcgcttctat tcagtcctcc ctgcaaacgc tcattcctgt ctggaaaaca atgattcaaa
     1501 acgatcccct agtcgcaaaa gacctggctc tcaatggcaa tgatattcag gagcttggtt
     1561 tttccggaaa agagattgga acagcccagg cgtctgcact cgagtattta tatagatggc
     1621 ctggtcgaaa cacgcgcgaa gatctgactc aattcctgct tggaaaaaga tccagcagct
     1681 cgtgaatcga atcaattcga gccaccgaac ctccccattc ttcttccgtc ccatgcccga
     1741 agcgggccgc tacaaatggg acctggttct cgatcgccgc atcgcgatcc gatgcacgat
     1801 ctccgatcaa cgctgcctgt gcgggcatta gtaaattttc tttaagaatg cttgcgacga
     1861 gctccgattt attctgaatg gtgccgccca ccgttggaat tgatttgaac tccggaaaga
     1921 ttccgccagc ctgcaggatg gatcgaacgt aaggtgctct cccattcgat gcagtgaaca
     1981 gatcgtatcc tcgcttcttc aactcacgga ccgtttctgt tacgcccggg taatggtgcc
     2041 cgcgccccgc tgcaattcca gtcaccagac cttctagaat tcgcccggat agttcatcct
     2101 gttccgggcg cgaaaggtct ggagccagat ttgcaaaaat cacgggcact ggctttccga
     2161 tctgttcgat gatttccggc ctggtgggta tatgagccgg tcttccgagg agagccctgg
     2221 ttttttcaaa ttcttcgata tagatctcgt gaatgatttc ctcgcttgag aaaatcgttc
     2281 cgtccacatc gaagagaatc gctttgcagt cgcgaagacc gctcacttct tatcgcctag
     2341 aacgcgaatc aaatcatctc ttggcacgaa ttcgccacgc ctgatccttt cgagtacctc
     2401 ttccttgctg agaggtttca tttttggatc ttcttttgcg tctgatggca acacaacatc
     2461 tccgatgacg ggcaatcctt tctcgatcgt cgcagacacg tattctgcat tctgccctgt
     2521 tgcgcgcagc gcgatatctc ccttcgcatt cttcaatacc tggcccgttg caaatttacc
     2581 tgtaggcagc ccgtaaagcg ccacctcttt gttgtccttg tccaccttgc aaacaactgc
     2641 ctgaggaaat cgcccaaatc ctgatggatt tcctctataa aacgtccccg tcttgccgct
     2701 cagagtgttt ccatcctctg agagctcgag taaatccaga ttgtaaccgt cttcaatttt
     2761 ccgttgatct tcttcgggac caaactgatt ggccggccag atctgctttc cttttacatt
     2821 ttcaagaacg gatctcttgg agctgagata cacttgttgc gaaagcaaca cctctttgcc
     2881 gcttgtgcga agatccccgg ttcttctggt aacgaagagt gtctgatcgt tccaggcaag
     2941 attgcgcgct actgtgcgaa cgaaactgaa ggtatagggt gcagcccccg gagttttaga
     3001 tgcagatttc tctacccaaa gaatcgtaaa atgatcctcg cctgctttcg ctgtgtcctg
     3061 gatccacaac ccctccactg gaaaatttgg tggagcctct gccgatcgca gctgcggact
     3121 acaggctgcc acactcagag ctattaccat tgctgccagt ttcatgcgga tgaaaatgca
     3181 caaacacgcc ctgcatgaat acaaaaaaaa cttccgcccg gcgccccggt ctcgagcatg
     3241 cacctgtggt tctaacagat tacagaatcg aagcagggac attctctggc gcaggtggat
     3301 tgactctccg ctacaaagcc tggatatgcg aagacgtaga tagcggacga aatcttgttt
     3361 tccatcacgg ctttggagaa cattgcggaa gatacgaata cctggtagaa gcgctggcag
     3421 gggatcgcgt aaacttgttt tcatttgatg cacgcgggca cggaagatcc gaaggaatca
     3481 gaggtggtgc cggtgacatt acagacatgg tgcgcgattt agaacacttc atgactttct
     3541 tgcgcgaaga gttcggagta agatgccctc ttttgctcgg tcactcgatg ggcggactcg
     3601 tggttctgtg ctttgctctt catttctcta atcaatggga gacccggggt cttttgacaa
     3661 gcggcgcggc gctgaaggtg aaaatgaatc cggtcctgcg catcaagcaa gtttccggtc
     3721 aaatcattgg acagtttgcg ccagacctgg ttttacctgc tggagtggat ccaaatctac
     3781 tcagccacga tccgcgcgaa gcgcaggctt acgaagatga tcctctcgta catggaaaca
     3841 tcagcattcg tctgggcctg gacatggtgc gtcagggcaa gctggccatc gaaaacgcgg
     3901 cccgcataaa agtgcccatt tttatgggcc acggcggagg cgatgaagtg gcagaccctc
     3961 tgggaactcg tcaatttttc aagggcgttc agtcagaaat cgcagagctc cgcatctatc
     4021 ccgggcttta ccacgaaatc ttcaatgaat ctcccgatgc tcgctccgct gtgtttgatg
     4081 actatcgcag attcatacag aacattctgg cagccacaga aacaatcctg ccgccagtcg
     4141 aacctgcgac cgaaagttag ccaaaaagca gaaatcgggc aaaatcgggt ttgacctcga
     4201 aggtagaatc cggtctcatt tccccaccac aaggagggga cctcaatgga aaaaattctc
     4261 caggacattc tgaacgcagg catcgcactg tttcgcgcag gcgaaggaac agtccagaac
     4321 gcagtcaagg aagttcagcg cacgtttgag caactgaaag aaaagggagc tcaagacaat
     4381 agcgagcaag cagtgaagct acgcaaaacg ctcgacgata tcgttcatca gacatccgga
     4441 ctttcgtcca aagcaggcgc tgcctactcg gacgcactgg ctcagattga gcagctctac
     4501 aagaacgtag gcgatcaaat ccagaagctt gtgccaccag aaagaatcaa cgaagtcaaa
     4561 gataagatcg aagaactcac tcgtgtcatt cagcaaagag ctggcggcgc tccaaaagtt
     4621 taatccacaa tccgctccgg aaacggagcg gagcttttcc ttcatggact acgaatcaaa
     4681 acaagaccac atccgcgaac tcaaaacgcc agacatagaa gtcttccgta acatatacaa
     4741 cgaccgcgaa tacagaatcg acatggagat cccggagttt acggcagtct gtccgaagac
     4801 cggccttcct gatttcggaa tcctgtacct ttcttacaaa ccggcggaac attgccttga
     4861 gctgaagagt ctcaaggaat atgtcctctc ttacagaaat gttggtatct tccacgaaaa
     4921 cgtagtcaat cgcatcctgg atgatattgt aaaagcatgt tccccgagat gggcacgcct
     4981 gcatggcgac tacaacgtgc gtggcggaat caagactcgc gtaacggcag tttatcggcg
     5041 tccctgagta tctcctcaaa gcctgagcgg taatccggat atctaaatcg aatcaactga
     5101 tccgcataac gactgattat gcgccgtccg gacggcaggt ccgttgccca gcttcccgga
     5161 actttcacgg acagcagatt ctcaagatat gcgaagactt ctgagttgag cgcggaattg
     5221 ccgtctactg cgttgatcaa atctatatca gacgtggccg cggccaacgc cagctgtgcc
     5281 agatcgtgca cgtgaattct ggataccatc ctgtttcctg aatctgcacg gccaaattgt
     5341 ccggccagaa actgcagcgg cagggctctg ccaggaccgt agatgccacc tgccctcaag
     5401 atcactgcgt ccggaaaact ctctttgatt ctttcttcga gcgccagcct gcgctcgcct
     5461 cttacggttt ccggtcgggc cggagtgaat tcgtcaaagc aaggaagggt tttctcttcc
     5521 ccgtgaaaat cctccgcata tacgcttgtg gaactgatgt gaaccagtct gcatctttgc
     5581 gcagaggaag attttccaag gaccagttcg atctcactcc tgtacggcaa atcaattcga
     5641 ccgttttctt cgactgcagg gattgtatct agaatcgagt cgtaactcaa cgcaggatca
     5701 aagggcaaca aagcttggga aacaagggcc tcgggccgcc gagtcagaaa tcggacctca
     5761 acggaaggga actcgtgatg gagaattttg cccgtgtaac tagcaccaca gacgagcact
     5821 ctcaaccgtc tacgtctccg gcaggcgctt tcttgatcag gag
//
LOCUS       DAOQJL010000201         5859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7773_length_5859_cov_4.459855, whole genome shotgun sequence.
ACCESSION   DAOQJL010000201 DAOQJL010000000
VERSION     DAOQJL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7773_length_5859_cov_4.459855"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLG78_10000"
     CDS             complement(<1..503)
                     /locus_tag="PLG78_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23491.1"
                     /translation="MKFSRFRAVFILGSLALGAVLFYITFFVYGGFQSSITFNGGIRF
                     SLVLPAGLGKADLEKAALKAGVDNPQIKLSGFRTNQYDLEIGPKTKEKLEKELAALKP
                     SEKPKGDGGVTAELESRILGNLGLTPRSVVSRETMAASYGDELWRISLWSLFWCIIII
                     GIYLSFR"
     gene            complement(500..2428)
                     /gene="secD"
                     /locus_tag="PLG78_10005"
     CDS             complement(500..2428)
                     /gene="secD"
                     /locus_tag="PLG78_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNE23492.1"
                     /translation="MRSLASRTIVVVFTMGACVLLMLPNFLTQKLAVVFQENHRLEDG
                     KKVPVDLAEVRDFMRDRENGLGLYFPSAQCVPEAGDGIPAERRCVLEARFITAAAVNE
                     MAQAHPDLVDEFRTRLLPHPIEKFFGFLSDKNIKNLKIRLGLDLQGGMRATFRADFDK
                     HIASLKERYEPLIADLEGKLARSSPEEKKNLEARIANLKSQMTITEQKKQDLLQEARR
                     IIDARLASQNLTEPELRVQPGSYSIGVDLPGMANSSEVLARIRDTVTVEYRLVNDEAQ
                     AKLGRVQGLDKDLADIQKMYIESRVDQDEIKRILTSITEKAQIRPDEGKVFLRWRRGI
                     KDGSQSLPRSFVVLGPVQLDGNDMTEAHANPSQGSPYYEISFVLSAEGAAKFGDLTTK
                     NVGKQIAIVWGDRVVSSANIKEPIIGGVGRISGDFTVQDAKEISDVIREGALPLNLEI
                     LSVSFVGPSLGQESIIAGVISLMLSFLLVIMFMAGYYRWCGVVAVTALFFDMIIMTAV
                     LSFLEFTLTLPGFAGLILTVGMAVDANVIIYEKMKEDIRAGKTASLAIEGGFRASFWT
                     IFDSHVTTLISSIILFKIGDGPIKGFAIVLFFGLITSLFTSLYVSHLIFDWFSLLFRP
                     ERLSIGWGYSRYAQGGKK"
     gene            complement(2436..2810)
                     /locus_tag="PLG78_10010"
     CDS             complement(2436..2810)
                     /locus_tag="PLG78_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23493.1"
                     /translation="MRWLPLLLITAGLYAQEPQETPKPQEEPRKQEAPRKPPVAAPLP
                     KVQGLSDDFPIGDEKYLPEYKGETIAPPKETAQPEKKPDDSPKGPGFLAGLLSGDKTF
                     LNVLILCLLVGIFVLYRLRGRK"
     gene            complement(2812..3183)
                     /gene="yajC"
                     /locus_tag="PLG78_10015"
     CDS             complement(2812..3183)
                     /gene="yajC"
                     /locus_tag="PLG78_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNE23494.1"
                     /translation="MEFLRFLAQQPAAPPTTEAPAQAPGAAAAGPGSSLTMFLLPVAI
                     FAFMWLFVIRPQRKEEKKKREMVNTLKKGDEVVTTGGMIGTIATIKDDTVILNVGDKT
                     RLEFMKSHISQMRETKKAEAK"
     gene            complement(3234..4271)
                     /gene="trpD"
                     /locus_tag="PLG78_10020"
     CDS             complement(3234..4271)
                     /gene="trpD"
                     /locus_tag="PLG78_10020"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNE23495.1"
                     /translation="MQEYLKKVVSRQDLTAEEASHAMTAIMTGEAGEISTAALLTALA
                     MKGETPVEIEAFARSMRKAAVAWTGRSEDAILDTCGTGGDRSNTINLSTLSALVLASA
                     GLKVAKHGNRAVSSSTGSADLLEALGISLGVQSPEVLDQCLNQTKIAFLFAQAWHPAM
                     KHAGPVRRALGFRTVFNLLGPLTNPAPVTHQLVGVFDARFLEPVAATLLHLGRKAAYV
                     VHAENGLDEISPAGGTRYFRVQDGKVESGTWKPEDFGFSSFPLSAIVAKDKAASVDRA
                     RAIVSGQGTQEENAAISMNAAPLYAAVRGVDLKTAAGACEQLLKQGAPGKVLEAWQKF
                     TSEKDLSVGAG"
     gene            complement(4271..4960)
                     /gene="pgsA"
                     /locus_tag="PLG78_10025"
     CDS             complement(4271..4960)
                     /gene="pgsA"
                     /locus_tag="PLG78_10025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNE23496.1"
                     /translation="MNLPNFLTVLRVISVPFFIYFLVQPSFTWRLTAFILFALASLTD
                     LVDGYLARKWNQQTEFGKFLDPLADKFLVLGAFITFVVLTEQVQVWMVLCIVGRDMLI
                     TALRYLGIRKGKSLRTSRFGKWKTALQMFSIVIILMSFLLVSYGDKKEINRIYAEGRS
                     QGIGPFDVAWFNLQEAMAGNHADWFYSLASFLPYYLMLITTIITILSGLRYLVTNYKL
                     LLPPYRVKEKV"
     gene            complement(4957..>5859)
                     /locus_tag="PLG78_10030"
     CDS             complement(4957..>5859)
                     /locus_tag="PLG78_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE23497.1"
                     /translation="QAQGKPYAPVKISDGCDRGCSFCAIPLFRGKFRDIPADEIIAEC
                     ERLAAQGVREVFLVSQDSNSYGGKPEAYLDLVERIHEVQGLHWIRMLYMYPDGKTERI
                     LEGMRTRSMPKVVPYLESPVQHASESVLRAMRRIGSSERFADLFALARDVMPGCEVRT
                     SILLGFPGETDADVEKVEQFIATAKPEKLALFSYSPEEGTPGAEMSAQIAPEIIADRI
                     RRVQSVHRGVQSEHLRSLVGTTFTCMVDESSAEEVVLRRPQDAPEADGVVYAEPKSRL
                     LHSGDLVNVEITGFTEYDLTGVIT"
BASE COUNT         1490 a         1548 c         1406 g         1415 t
ORIGIN      
        1 aatcgaaagc tcaaatagat cccgatgatg atgatacacc agaacagact ccagagggag
       61 atcctccaga gttcgtcacc gtagctggcc gccatggttt cgcgcgacac tacactgcgc
      121 ggagtgagac ccaggtttcc aagaattctg ctttcgagtt cagcggtcac tccgccgtct
      181 ccctttggtt tctccgaagg tttgagggca gcgagttcct tttccagttt ttcttttgtt
      241 tttggtccaa tctccagatc atactggttt gtgcgaaagc cggacaactt gatctgagga
      301 ttgtccactc ctgctttcaa cgcagctttt tcaagatccg cttttccaag accggccggt
      361 aaaaccagac tgaaacgaat gccaccgttg aaagtgatcg acgattgaaa accgccgtac
      421 acgaaaaacg taatgtaaaa cagaaccgca ccgagtgcga gagagcctaa gatgaagacg
      481 gcgcgaaagc gtgaaaattt cacttcttgc ctccttgcgc gtagcgagaa tatccccatc
      541 caatagaaag gcgctccggc cggaaaagca gactgaacca gtcaaagatg agatgtgata
      601 cgtatagaga cgtaaacaga gacgtgatca gaccaaagaa cagcacgatg gcgaagcctt
      661 tgattggccc atctccaatc ttaaatagaa tgatggaaga aatcaatgta gttacgtgcg
      721 agtcaaagat cgtccagaaa gaagctcgaa agccaccttc aatggcaaga gaggctgttt
      781 tgcctgctcg tatgtcttcc ttcatttttt cgtagatgat cacgtttgca tccacagcca
      841 tacctacagt cagaatcaac ccggcaaaac caggtagagt gagagtaaat tcaaggaaac
      901 tcaatacggc cgtcatgatg atcatgtcaa aaaagagtgc ggtgaccgcc acaacgccac
      961 accagcgata gtaacctgcc atgaacataa tcacaagcaa aaacgaaagc atcagggaaa
     1021 tcacaccggc aatgatggat tcctgaccca gacttggacc aacaaaggaa acagacagaa
     1081 tttccaggtt cagcggaaga gcgccttcac ggatcacatc agaaatctct tttgcgtcct
     1141 gaactgtaaa atcgccgctg attcttccca caccgccaat gattggctct ttgatgtttg
     1201 cagaagaaac aactctatct ccccagacga tggcgatttg ttttccaacg ttcttggtag
     1261 tgaggtcccc gaacttggcc gcaccttccg cggacaatac aaaactgatt tcatagtaag
     1321 gagaaccctg ggaaggattc gcatgagctt ctgtcatgtc gtttccgtcc agttgcactg
     1381 ggccaagaac tacgaaagat cttggaagag attgagagcc gtctttgatc ccgcgtctcc
     1441 agcggagaaa gacttttcct tcatccgggc gaatctgcgc tttttcggta atcgaagtca
     1501 gaatacgttt gatctcatcc tgatccacgc gcgattcaat atacattttt tgaatatcag
     1561 ccagatcttt gtccagtccc tgcactcttc cgagctttgc ctgcgcttca tcgtttacga
     1621 ggcgatactc caccgtaaca gtatcacgaa tgcgagcgag tacttcagag ctgtttgcca
     1681 taccaggcaa atccacacca atgctgtaac ttccaggttg cactctcaac tcgggttctg
     1741 ttagattctg cgaagcaaga cgtgcgtcaa tgatgcgacg agcttcctgg agaagatcct
     1801 gcttcttctg ctccgtaatg gtcatctgac ttttcaggtt tgcaatgcga gcttcgaggt
     1861 ttttcttttc ttccggagaa gaacgcgcaa gctttccttc cagatccgcg atcaatggct
     1921 cataacgttc tttcagagac gcgatgtgtt tgtcaaaatc cgcgcggaag gtggcgcgca
     1981 tgccaccctg caagtcgagc ccgaggcgaa tcttaagatt ctttatattt ttatctgaca
     2041 ggaatccgaa gaatttctca attggatgag gcaaaaggcg agtgcgaaac tcatccacca
     2101 gatcaggatg cgcctgcgcc atttcgttca ctgcggcggc cgtaatgaaa cgcgcttcga
     2161 gaacgcatct tctttctgca gggattccgt ccccggcctc tggaacgcac tgagcggaag
     2221 gaaaatacag acccaggcca ttttccctgt cgcgcatgaa gtcgcgcact tccgccagat
     2281 ccacaggcac tttctttcca tcttccagcc tgtggttttc ctgaaacact acagccagtt
     2341 tctgagtgag aaagttgggc aacatgagga gcacgcaggc tcccatagta aatacaacta
     2401 caatggttct tgaggcaagt gatcgcataa cggtcctatt tacgtccccg aagtctatag
     2461 agaacgaaga tacctacgag gaggcagaga atgagaacat tgaggaaggt cttgtctccg
     2521 gagagaagac cggccagaaa tccaggacct ttaggagagt cgtccggttt cttttcaggc
     2581 tgcgccgttt ctttaggggg tgctatggtt tcccctttgt attccggcag atacttttca
     2641 tcaccaatgg ggaaatcatc agacagcccc tggacctttg gaagaggagc ggcgaccggc
     2701 ggtttgcgcg gcgcttcttg tttgcgcggc tcttcctgcg gctttggtgt ttcctgcggc
     2761 tcctgagcgt agagcccggc ggtgatcaga agaagcggaa gccagcgcat atcactttgc
     2821 ttcagctttc tttgtttcgc gcatctggct gatgtgcgac ttcatgaatt caagacgagt
     2881 cttgtctcca acgttgagaa tcactgtatc gtctttgatt gttgcaatgg tgccgatcat
     2941 gccgcctgtg gtgactactt catcgccttt cttcagagtg ttcaccattt cgcgcttttt
     3001 cttttcttct ttgcgctgag gacgaatcac gaagagccac atgaatgcga aaatcgcaac
     3061 tggcaaaagg aacatggtca gagatgaacc tggacctgct gctgccgcgc cgggtgcctg
     3121 tgcaggcgct tctgtggtag gaggggcggc tggttgttgc gcgagaaaac gtaagaattc
     3181 catgcaacca gacgcccgat tctcgcctgg cttgcaacta taaacttctg aagttagccc
     3241 gcgcctacag aaaggtcttt ttcagacgta aatttttgcc aggcttccag caccttgcct
     3301 ggagctcctt gcttgagcaa ctgctcacag gccccggccg ccgttttaag atctacaccc
     3361 cgaaccgcgg catagagagg agccgcattc atagagatgg ctgcgttttc ttcctgagtg
     3421 ccctgacctg aaacgatcgc tctggcgcga tccacggatg ccgctttgtc ttttgcaaca
     3481 atggcagaaa gtggaaaaga gctgaatcca aaatcctctg gcttccacgt tccactttcc
     3541 actttcccat cctgaactcg aaagtagcga gtgccgcccg cgggcgaaat ttcatctaaa
     3601 ccgttttctg cgtgaactac gtaagctgcc tttcttccaa gatgaagaag agtcgccgcc
     3661 acaggttcga ggaatcgcgc atcaaacact cccaccagtt gatgggtgac tggcgcggga
     3721 ttcgtcagag ggccaagcaa gttaaagact gtgcgaaagc caagagcacg ccggacaggg
     3781 ccggcatgct tcatggctgg atgccaggcc tgagcgaaca agaacgcaat tttagtctga
     3841 ttcaggcatt gatcgagcac ttcaggactc tgcacaccca gagaaatgcc cagcgcttcg
     3901 agcaaatcag ccgagccagt ggaactggac acagccctgt ttccatgctt ggccactttc
     3961 aacccggcag acgccagaac aagagcagat aacgtagaaa gattgattgt attgcttctg
     4021 tctccgcctg tcccgcaagt atcgaggatg gcgtcttctg atcgaccggt ccacgcaact
     4081 gccgcttttc tcatggaacg agcaaacgcc tcaatttcaa cgggagtctc ccctttcatg
     4141 gccagggcgg tcagcaaagc ggccgtcgag atttctccgg cttctcccgt catgatggca
     4201 gtcatcgcat gcgacgcttc ttctgcagtg agatcctgcc tgcttactac tttctttaaa
     4261 tattcctgca ttacactttc tccttcacgc gatacggcgg cagaagcagc ttatagtttg
     4321 taacaagata acgaagccca gaaagaatgg tgatgatcgt tgtgatgagc atcaggtaat
     4381 atggtagaaa gctggccaga ctgtaaaacc agtccgcatg atttcctgcc attgcttcct
     4441 gtaaattgaa ccatgcgaca tcaaacggcc caattccctg cgatcttcct tctgcgtaaa
     4501 ttcgattgat ttctttttta tctccgtacg aaaccagaag gaaactcatg agaatgatga
     4561 cgatgctgaa catttgcaga gcagtcttcc atttgccaaa acgagacgtg cgaagagact
     4621 tacctttgcg aattccaaga taacgaagag ctgtgatgag catatcacgc cctacaatgc
     4681 acaaaaccat ccatacctga acctgttcgg taagaacgac aaacgtgata aacgcgccga
     4741 gtaccaggaa cttgtccgca agcggatcga ggaactttcc aaactctgtt tgctggttcc
     4801 atttacgcgc gaggtatccg tccacaagat ctgtaagaga cgccagagca aaaagaatga
     4861 aagcagtcaa acgccaggtg aaagaaggct gcaccagaaa gtaaatgaag aatggaacag
     4921 agatgacgcg cagtacggtt agaaaattgg gcagattcaa gttattacgc ctgtcagatc
     4981 gtattccgta aagccggtga tctcaacatt tacaaggtcg cccgaatgga gcaaccttga
     5041 tttaggctct gcgtatacga ctccgtcagc ttccggagca tcctgtggac gccgcaaaac
     5101 gacctcttct gcgctcgatt cgtccaccat gcatgtaaaa gtagtgccga cgagggagcg
     5161 taggtgctcc gactgaacac ccctgtgcac gctctgcact ctgcgaattc tatctgcaat
     5221 gatttccggg gcaatttgcg cggacatctc tgccccggga gttccttcct cgggagaata
     5281 cgaaaacagc gccagcttct ccggcttcgc ggtggcaatg aactgctcta ccttttctac
     5341 atccgcatcc gtttctcccg gaaaaccaag tagaatcgaa gtgcgaactt cgcatcctgg
     5401 catgacatcg cgagcgagag caaacagatc tgcgaatctt tctgaacttc caattctcct
     5461 catcgcgcgc agaacagact cagatgcgtg ctgcaccgga ctttctagat acgggaccac
     5521 tttaggcata gagcgagttc gcataccttc gaggattcgc tctgtctttc cgtcaggata
     5581 catatacagc atgcgaatcc aatgcagtcc ttgtacctca tgaattctct ctaccagatc
     5641 caggtatgct tccggttttc cgccgtacga attggaatcc tgactgacca ggaagacttc
     5701 gcgcactccc tgcgccgcaa gcctttcaca ttcagcgatg atctcatctg ccggaatatc
     5761 tctgaacttt ccgcgaaaga gtggaatcgc acagaaagaa caaccgcgat cgcatccatc
     5821 tgaaattttc acgggcgcat acggctttcc ctgcgcctg
//
LOCUS       DAOQJL010000202         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7786_length_5854_cov_5.023107, whole genome shotgun sequence.
ACCESSION   DAOQJL010000202 DAOQJL010000000
VERSION     DAOQJL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_5854_cov_5.023107"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLG78_10035"
     CDS             complement(<1..419)
                     /locus_tag="PLG78_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE23498.1"
                     /translation="MRILIVEDEPIHARYLSHTLKEIPATAEAVVHTQKTLTAAECYL
                     LDHPVDLLFLDLNVYDGDGFTLLQGFAGAQFATIVVSAHPERAIEAFECGVLDFLPKP
                     ITKARLLTAIERYEKHRYDSRHTLKFFSIFADGVLETL"
     gene            complement(400..2304)
                     /locus_tag="PLG78_10040"
     CDS             complement(400..2304)
                     /locus_tag="PLG78_10040"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23499.1"
                     /translation="MNKASVKAVCLFVFVAIPGCGRSAGEPAVVQGQMDLSNHELLDG
                     QRIKLDGAWEFYHGQFLVSGQFPQRTLDFLSLPGYWNERGATVDEYPGHGFATYRAHL
                     RLHPRDVKRRLALYVPHAFTSYQLYLNGELVSQSGKPGRTAQEATSRFLPQAAFFQSQ
                     SPDVEIVIHVANFTSLKGGFRQSIELGTEREIMSYKQMLLAQDLFLIGGMLVIALYHL
                     YLYFLRRGESYALCFAGFVGTFLLYRITTGEYFIGMLFPGIQQPAVIKTFFFSLFTAP
                     PLFVTLLLRIYPDETNRFIARMAQLTGAALVLSLLYPGTQLAEHLLLPAEIVILIVGV
                     YCTAVLAFAFAHRREGATGFLLGFAFFFITVINDILFERDIIQTEVFAPLGLFVLLMA
                     DSLMLLRRFVSLIDTVEKQQAALVGAAELKERLTRARLQTKRMEFEMLKKAIQPHFLS
                     NSLAAIRGWLLENPEKSANLLDDFAGEIALIQAMVGQKDVPLADEIRLCRLHLHVMEA
                     RREKIYSFRARGMTGHERVPPMIFHTMLENAFTHLDTENGSLRFSLRKTSSKSTTEVV
                     SRFAFAVSNHDAGSIRQRKRGNGIGLRYVQARLEESFPGSWTLEQKRCRKGYLIRINI
                     KEGADANTDR"
     gene            2602..3633
                     /locus_tag="PLG78_10045"
     CDS             2602..3633
                     /locus_tag="PLG78_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23500.1"
                     /translation="MQGCPLNLQGRVVTLAGSGFLGGDVEGRGSSAKFSGLGAWAANG
                     SFAFGGDIGNKKIKKIDLRSADVAIIAGPAAGAVVSGDADGSSAVSTFNDPQMTTLSG
                     NALYVVDGTNNKIRRIDLATGIVSTFAGPSSGTTTFGDTDGVGNAARFFAPTGITTDG
                     TNLYVVENGNCKIRRVSLATQQVTTIVGPLPGSNGCSLDNDGIGNAARLFAPGAITTD
                     GKNLFVSDNSKIRKVDLNTLQVTTIAGPAPGSSGTGHVDAAGTAARFGSILGLATDGF
                     SLFVSESYYVRVLNPGTQVVNTLAGDGNLGLTDGVGPGAQFTTQLRLSASDGAHLILG
                     DGNNVRLLE"
     gene            3683..5443
                     /locus_tag="PLG78_10050"
     CDS             3683..5443
                     /locus_tag="PLG78_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE23501.1"
                     /translation="MIANNYFLDDPDLQTFFNHLIDWDSIVQAVEGNDFADHRRFLET
                     GNSRFEMAPASVEEAVGLYRSSLESLGEFCGKELSQVSQAMDRAALQFQNGRVVFPSE
                     TISVYEKFRETGLMPYSISRHAGGLGLPATVGAFIVMLLARADVSFCMTITLLNLAQI
                     VARYGTEEQIEQFAAKAAAGETLFAMALTEPDYGSDLNSVRMTAIRSEDGSYRLNGTK
                     RFISQGCGLGPYPCTLLTLARTGKAGGGARGLSVFLVKSEDVTIAGIESKMGIHASPT
                     CEVVYEDAPGQILGEEGLGLTRYTAGMTNFMRLGSAAGGPGGGAGAYFECEKYASSRI
                     QFGKPIAQIPAVAEMIQKIRREVNAMRLLAFETARIVDLFQHHQIRLEKEGVEDRDIR
                     RDETIKTWSTLASILTPVAKYYCSEEGHRCGNLAVQIHGGAGYTEDYDVSRMFRDSRI
                     NTIYEGTSQIHVRIATGAISAGMGEGANFRKYLEDLYSQVRAPSTFLIEQFRLLQESV
                     DCFRMIESEDSRERLAENLMIVAARFLCTVLYEKALGLLANHPDLERWRADCRSYGLE
                     SAAIASGCFYRLKNSEFAAV"
     gene            5537..>5854
                     /locus_tag="PLG78_10055"
     CDS             5537..>5854
                     /locus_tag="PLG78_10055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE23502.1"
                     /translation="MELQAITPRSTFRVSFHDCDAFGHLNNARYISHFIDARTDHLRD
                     FHGYDVYADAEKTGKNWVVQNTSIRYLYPAKLNQLVEIETRLLRFEKHRLFPEGIMRD
                     PETG"
BASE COUNT         1391 a         1404 c         1616 g         1443 t
ORIGIN      
        1 agtgtttcga gtacgccgtc tgcgaagatg gaaaagaatt tcagcgtgtg cctggaatcg
       61 tagcggtgtt tttcatagcg ttcgatggca gtgagcagac gtgcctttgt tatgggcttt
      121 ggaaggaaat ccagaacacc gcattcgaat gcttcaatgg ctcgttcggg atgtgcggat
      181 acaacgattg tggcaaactg cgcgcccgcg aatccttgca gtagagtgaa tccgtctcca
      241 tcgtagacat ttagatccag gaaaagaaga tccaccgggt gatcgagcag gtaacattcc
      301 gccgcggtga gtgtcttctg tgtgtgcact actgcttccg cggttgccgg gatttcttta
      361 agagtatgac tcaaatagcg cgcatggatt ggttcgtctt caacgatcag tattcgcatc
      421 cgcaccctcc ttgatgttga tcctgataag gtaacccttt ctgcatcgtt tctgctcaag
      481 tgtccaggac ccggggaagg actcttcgag cctggcctga acataacgca ggccaattcc
      541 atttccgcgc ttacgttgcc gaatcgagcc tgcatcatgg ttgctcactg caaatgcaaa
      601 gcgagacacg acctcagtgg ttgatttcga ggaagtcttt cgcagtgaaa agcgaagcga
      661 accattctct gtgtccagat gagtgaatgc gttttcgagc attgtatgaa agatcatagg
      721 aggaacgcgc tcatggcccg tcattcctcg ggcacggaaa gaatagatct tctctcttcg
      781 cgcttccatg acatgcaaat ggagtctaca aagacggatt tcatctgcca gtggcacatc
      841 tttctgaccg accatagcct ggatgagagc gatttcgccc gcgaaatcat caagtagatt
      901 ggcggacttc tcgggattct caagtagcca gccacggatt gccgccagcg aattgctcaa
      961 gaaatgtggt tgtatggctt tcttaagcat ttcgaattcc attctctttg tctgcaatct
     1021 cgcccgtgta agcctctctt tgagttctgc cgccccaacg agtgctgctt gctgtttctc
     1081 cacagtgtcg atgaggctca cgaatctgcg cagaagcatc agggagtctg ccatgagaag
     1141 aacgaacaaa cccaggggag caaacacttc tgtctggatg atatcccgtt caaagagtat
     1201 gtcgttgatt actgtaataa agaagaatgc gaaacccaat agaaatccag ttgccccttc
     1261 gcgccggtgc gcgaatgcaa aggcaagcac cgcagtacaa tacacaccta cgataaggat
     1321 tacaatctca gccggcaaaa ggaggtgttc cgccagctgc gttcctggat atagcagcga
     1381 taagacgagc gctgcccctg ttaactgtgc catacgtgca atgaatcgat tcgtttcgtc
     1441 cggataaatt ctaagaagaa gcgtcacaaa caatggtgga gccgtgaaca aggagaaaaa
     1501 gaatgttttg atgacggccg gctgctgaat cccaggaaag agcattccta tgaagtattc
     1561 gccggtagta atcctgtaga ggagaaacgt tcccacaaat ccagcgaagc acagtgcgta
     1621 cgattctccg cgtctgagga agtagagata cagatgatac agcgcaatca caagcattcc
     1681 accgatcaaa aacaggtctt gagcaagaag catttgcttg tagctcataa tctctctttc
     1741 agttcctagt tcgatgctct gtctgaatcc tcctttaaga gatgtgaagt ttgccacatg
     1801 aattacaatt tcgacgtccg ggctctgact ctggaagaac gcagcctgtg gaagaaacct
     1861 ggaagttgcc tcctgtgctg tacgtccagg cttgccgctt tgtgaaacaa gctcaccatt
     1921 caggtagagt tggtacgatg tgaaagcatg cggcacgtag agggctaatc gtcgcttaac
     1981 gtctcgaggg tgaaggcgca gatgcgcgcg atacgttgca aaaccgtggc ccgggtattc
     2041 gtcgactgtt gctccccttt cgttccaata gcccggaaga gaaagaaaat caagcgtacg
     2101 ttgtgggaat tgtccgctga ccaggaattg cccgtgatag aattcccagg cgccatccag
     2161 tttgattcgc tggccatcga gcagttcgtg attggaaaga tccatctggc cctggactac
     2221 tgcaggttcc cctgcggatc gaccgcagcc aggaatggcg acgaaaacaa acaagcagac
     2281 agccttgacg gacgctttgt tcacaggtgg gcgaggtttt agtatgaggc cacgtctgca
     2341 atgaaattgt gatttttgtc aggtgcttct cgcgcccgtg gaagagcatt tcgcgcacct
     2401 cgcgttgacg gagggcgcct aacggtttct aatctgaggc ctatgttgcg acggccccgg
     2461 gtgaagctgt tcatttgtat aatttcttta acaatgcaat gcagcgtctc tggcgcctgc
     2521 aattctggtg atccaagctg tagcatagcg tccttcccgt tgtttctgct gacctgtaca
     2581 ggatacgcaa tgggtggagg catgcaagga tgccctttga atcttcaggg gcgcgtggtg
     2641 actcttgccg gaagtggatt tcttggtggg gatgtggagg ggcgaggaag ttccgcgaaa
     2701 ttctctggtc tgggtgcctg ggctgcgaat ggttcattcg catttggcgg agacattggc
     2761 aacaagaaga tcaagaaaat agatttgcga agcgcggacg tcgccatcat cgcgggcccg
     2821 gccgccgggg cagtggtcag cggagacgca gacggttcgt ctgcggtttc cacctttaat
     2881 gatccacaga tgacaactct ctctggcaat gcgctttatg tcgttgatgg aacaaataac
     2941 aaaattagac ggattgatct tgccactggc atcgttagca catttgcggg accgtcatcc
     3001 ggcactacga cttttggcga caccgacgga gtggggaatg cggcgcgctt cttcgcgccg
     3061 actggcatca cgactgatgg cacaaatctc tacgttgtgg aaaacggcaa ttgtaagatt
     3121 cgtcgggtct cgctcgccac gcagcaggta acgacgatcg ttgggccgct acccggaagc
     3181 aacgggtgta gtctggacaa tgatggcatt ggaaacgcgg cacgtttatt tgcgccgggc
     3241 gcgattacaa cggacggtaa gaatctattt gtttctgata acagcaagat tcgcaaagtg
     3301 gacctcaata cacttcaggt tactacgatc gcggggccag ctcctggttc cagtggaact
     3361 ggtcacgtgg atgcggcggg gactgcggca cgatttggtt ccatcctggg tctggctacc
     3421 gatggcttca gtctttttgt ttcggaaagc tattacgttc gcgtgctaaa ccctggcaca
     3481 caggttgtca acaccctggc tggagatgga aaccttggtt tgacagatgg ggtcgggcca
     3541 ggagcacagt ttacaaccca gctgcgtctt tccgcgtctg acggcgctca tttgatcctt
     3601 ggagacggca acaacgttcg tctgctagaa tgacgggttc ggaattgatc gtccttcctc
     3661 cggggtgttg cgattctggc gaatgatcgc aaacaactac ttcctcgatg atcccgattt
     3721 gcagaccttt ttcaatcatt tgattgattg ggattccatc gtgcaggccg tggagggaaa
     3781 cgatttcgca gatcacagac gctttctgga aacgggaaac tctcgcttcg aaatggctcc
     3841 ggcgagtgta gaagaagctg ttggtctcta tcgttcgagc ctggagtctc tcggtgaatt
     3901 ttgcggcaaa gaactatcac aggtttcgca ggcgatggat cgcgcggctc tccaatttca
     3961 gaatggccgc gttgtatttc cttccgagac aatctcagtt tatgaaaaat tcagggaaac
     4021 ggggctcatg ccttattcta tctcgaggca tgcgggtggc cttggcttac cagcaacggt
     4081 cggtgctttt attgttatgc ttctggcgcg tgcggacgtt tccttttgca tgaccatcac
     4141 tctactgaac cttgcgcaaa ttgtggcaag atacggaacg gaagagcaga tcgagcaatt
     4201 tgccgcaaaa gcagctgcag gggaaacgtt attcgccatg gctttgacag agccagacta
     4261 cggatccgat ctcaacagcg ttcgcatgac tgcaatcaga agtgaagacg gtagctatcg
     4321 attgaatggc accaagcggt tcatttctca gggatgcggt cttgggcctt atccatgcac
     4381 tcttctgacc ctggcacgca ctggaaaagc gggtggtggt gcgcgaggtc tttccgtatt
     4441 cctggtcaag agcgaagatg taacgatagc cggcattgag agtaagatgg gaatccacgc
     4501 ttcccccacc tgcgaagtgg tctatgagga tgcgcccgga cagatccttg gagaggaagg
     4561 cctgggttta accagataca cggcaggcat gaccaatttc atgagactgg gaagtgcagc
     4621 gggtgggcct ggcggcggcg cgggcgccta cttcgaatgt gaaaagtatg cgtcatcgcg
     4681 cattcagttt ggaaaaccaa tcgcgcagat tcctgcagtt gctgaaatga ttcagaaaat
     4741 ccgcagagag gtcaatgcaa tgcggctact ggcttttgag acagcgcgca ttgtcgattt
     4801 gtttcagcac caccagattc gtctggaaaa ggaaggcgta gaggatcgcg acattcggcg
     4861 cgacgaaacc atcaagacat ggagtacgct tgcgtcgatt cttacgccgg ttgcgaaata
     4921 ttattgttcc gaagaaggac atcggtgtgg gaatctcgcc gttcagattc acggcggagc
     4981 cggttatacg gaagactacg acgtctccag aatgttcaga gattcacgaa tcaataccat
     5041 ctacgaagga accagtcaga tccacgttcg catcgccacc ggcgcgattt ccgctggtat
     5101 gggggaaggc gcaaatttta gaaaatacct ggaggacctg tactcgcagg tgcgagctcc
     5161 gtcgactttt ctgatcgagc agttccgttt gctgcaggaa tctgtggatt gttttcgcat
     5221 gatagagagc gaagacagtc gtgagcgtct ggcagagaat ttgatgatag tggccgcaag
     5281 attcttgtgc acggtccttt acgaaaaggc ccttgggcta cttgccaatc atccggacct
     5341 ggaacggtgg cgggcggatt gtcgaagtta tgggctcgaa agcgcggcga ttgcgtctgg
     5401 ctgcttttac aggctcaaaa acagcgagtt tgcggcagtc tgaaaatacg tggatatcca
     5461 ctcttttttt gtggacaagt ttgttctgcc ttcttaaatg agtgtatatc cacctattgg
     5521 tcggatggag ggttttatgg aacttcaggc aatcactccc aggtcaacgt tccgggtgtc
     5581 tttccacgat tgtgacgctt tcgggcatct aaacaatgca cgctacattt ctcatttcat
     5641 tgacgcgcgc acggatcatc ttcgcgattt ccacgggtat gatgtgtatg cggacgccga
     5701 gaagacgggg aagaactggg tggttcagaa cacttccatt cggtatctgt acccggctaa
     5761 actcaatcaa ctggtggaaa tcgagaccag gcttttgcga tttgagaaac atcgtctgtt
     5821 cccagaagga atcatgcgag atcctgaaac agga
//
LOCUS       DAOQJL010000203         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7801_length_5844_cov_4.257730, whole genome shotgun sequence.
ACCESSION   DAOQJL010000203 DAOQJL010000000
VERSION     DAOQJL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_5844_cov_4.257730"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..724)
                     /locus_tag="PLG78_10060"
     CDS             complement(209..724)
                     /locus_tag="PLG78_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23503.1"
                     /translation="MQELTFDSFQDSKTISVSGQNVSAYPSGHGCVYFEWAFGNRSEK
                     KWESYSVGFQSEVQLNLKTENGKMTVSRADIRLYLNPAVEKYYTKESDAPEAVRAWIR
                     EHGSVSVAEYRLETGKKYYAVLHVDHYYLPRPGGPPSSHSKKVLWISDEPIKDGKPSV
                     EITPAYRGWTY"
     gene            complement(795..1601)
                     /locus_tag="PLG78_10065"
     CDS             complement(795..1601)
                     /locus_tag="PLG78_10065"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNE23504.1"
                     /translation="MTDADILSGLSDAVATAASAVLRVFNDKSFSVSSKGDAGPLTTA
                     DLESNRILESHLRRLLPDAGWLSEESTDDDDRLSCREVWIVDPIDGTKEFVAGQKDFS
                     VSVGLSRNGTPVLGAVAMPAEDILISGGMGVMKNGVRASISRRRELIGSTLLVSQTEF
                     QRGTFAELQNDFEIRPTGSIARKLAMLAGGEGDLVVSLYPKNDWDICGGTALVLAHQE
                     TMVTDLENRKARHYNQKDPKSFGLVAGNTDLVLQFHEYFLRRGLKLARKY"
     gene            complement(1598..2050)
                     /locus_tag="PLG78_10070"
     CDS             complement(1598..2050)
                     /locus_tag="PLG78_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23505.1"
                     /translation="MKRVIVIIALFSVPVWAGRKEGVQQEYRAVEEAIRNKQTGPEAR
                     LKTLETNLVNAVRMAMTRHYYEEKETIIKDLSIQTIEYENPTSPLVYFVKYKNSFFRF
                     EYARNPESFAQSPITEKFLDAAEVDMTHRESSTNPTAPATPAQPAKTP"
     gene            complement(2113..4299)
                     /locus_tag="PLG78_10075"
     CDS             complement(2113..4299)
                     /locus_tag="PLG78_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE23506.1"
                     /translation="MKTELKAKIEEELLANLNPVQKQAVLHNEGPLLILAGAGSGKTR
                     VITHRIAYLCRVLNVPPYRVCAVTFTNKAAGEMHQRLYGLIGPMADSVLVRTFHSLGL
                     YILRRNAPKLGLKSGFSIYDQNDQEALIKRVIKDLDITSSWLTPRAAAERINRARDQM
                     VSPEKFEQTADRYEEDVIPIYREYMIRLRKNNALDFGDLIYETVRMFEREPEVLADYQ
                     SMWRYFMIDEYQDTNHAQYMLGRLIARDHTNIAVVGDDDQSIYSWRGADIENILSFEK
                     DYPNCTVLRLEENYRSTAPILKAASSLIAHNSERREKTLFTRQPGGEKIHYTVYESDT
                     EEAAGAISRIKSLKQQGIPYAESAVFYRTNAQSRHFEELLRRDGIPYLLVGGFPFYDR
                     KEIKDLIAYLSVVVNPEDSISLMRIINTPARGFGEAGLARLEALSVTEGITLYDAMAR
                     AGELQGVRGSRAIMDLHSWFENWRGMNPARPSLVLESIIEKTGYLEFLAKDENPGESR
                     IENVEEFASSIQEFEESESEPNLSAYLQQISLYSEQGDAEGQDAVNLITLHNAKGLEY
                     RAVFFTGLEEGTIPHTFSLEDNIEEERRLAYVGITRAREFLYLSSSRFRRIQGSYQPR
                     LPSRFISEIDVSVFEEGIATRAQNTPRARDSMGSFAGVGRRPPPPSVQVVPVQIGDRI
                     RHKSYGPGTVVEQEDTVAGAKIGIQFDGDEKVRYFLARFAPIEKIE"
     gene            complement(4296..5417)
                     /locus_tag="PLG78_10080"
     CDS             complement(4296..5417)
                     /locus_tag="PLG78_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23507.1"
                     /translation="MDAGIVAELSRQSVIALGRTWPNPSVACVIQSKGQIFSGATEIP
                     GRRHAEIVALDLFDQSLPRDADGDLYVTLEPCSKFGRTPPCTKRLANYPNLRLHIGNL
                     DPSLSGEGLARLRESGLQIVDDSNGMGREFLEGFIGRTLGQGPRFHLKVASCDGIMGS
                     KEKRIMVSSEDGIAFGQMLRAKLDAVLVGPRTIFVDRPGLEARTRSPLRMKRTSGQDP
                     FVDAIIANDELAATNSNQPLRVFVLGRPFPGAETWFEKQKALTETTGREPLFLSVEPW
                     PGRWTHALPPLSHPSFMIELRDVLADRGLGEVLVEGGAGLFHAIMPGLGSRDRLYHLE
                     RSGTLEAGPDSVRVPSALLQARPMASYHAGEEKLGVYHF"
     gene            complement(5422..>5844)
                     /gene="secF"
                     /locus_tag="PLG78_10085"
     CDS             complement(5422..>5844)
                     /gene="secF"
                     /locus_tag="PLG78_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNE23508.1"
                     /translation="FALGASLSLVHDVILTIGFIGVARIEPSIPVVAAVLTIVGYSIN
                     DTIVIFDRIRENSKDRAQMSLRTTMDEAMTQTLSRTLVTSILTMISVLALLLGGADSL
                     RDFALVLIFGIFMGTYSSIFVAAHSVQYYEEFVARIRR"
BASE COUNT         1420 a         1561 c         1411 g         1452 t
ORIGIN      
        1 gatcgagtgc ttgtagacga gaatgctgcg accttcctgt tccaggagaa ccgtgaatcc
       61 atcatgagcg cgcacttttc cgcgcacggg cactccggtt ttcagataaa gagagatggg
      121 gcgaccggtt ttaagcgcgc cttcgaacaa ttcatcctgg attacctcag gcatggttca
      181 tcttccattg gttgcgtggc gcgtcaactc agtacgtcca accgcgatac gcaggtgtga
      241 tctccacaga tggtttgccg tctttgattg gttcgtcaga aatccagagc accttcttac
      301 tgtgcgatga aggagggcca cccggcctgg gaagataata gtgatccaca tgcaaaacag
      361 cgtaatactt cttgcccgtc tccagtctat attctgccac acttacagaa ccatgctctc
      421 gaatccaggc cctgaccgct tccggtgcgt ccgattcctt tgtgtaatat ttctccacgg
      481 ccgggttcag ataaagccgg atgtccgcgc gcgaaacagt catctttccg ttttctgttt
      541 tcaaattcag ctgcacttcg ctctgaaaac ccacgctata ggattcccat tttttctcag
      601 agcgatttcc gaaagcccac tcaaagtaga cacagccgtg gccggacgga taagcagata
      661 cattctgtcc cgaaacagaa atcgttttgc tatcctggaa cgaatcaaat gtcaattctt
      721 gcaacgaact ctcctggggc tcgcgcacgg ggcgggcaca ttctggaaat agaaaaaaac
      781 aaacgcaggc aacgctaata cttacgcgca agcttcaatc cccgtctcaa gaagtattcg
      841 tgaaactgca gaacaagatc cgtatttccg gcaacaaggc caaacgattt tggatctttc
      901 tgattgtaat ggcgagcttt gcgattctct aaatctgtaa ccatggtttc ctgatgcgcg
      961 agaacaaggg cagtgccacc acaaatatcc caatcgtttt tgggatacaa tgaaactaca
     1021 agatcgcctt ctccacccgc aagcatagcg agctttcgcg caatggaacc ggtgggtctg
     1081 atttcgaaat cgttctgcag ttctgcaaat gtccctcgct gaaattcagt ctggctcaca
     1141 agcaatgtgg atccaatcaa ctcccggcgg cgagagatgc tggccctcac tccgttcttc
     1201 atgactccca tgccgcctga aatcagaata tcttctgcag gcatggccac ggcgcctagc
     1261 actggagttc cattgcgaga tagtcccacg gaaactgaga aatccttctg acccgcaaca
     1321 aactcttttg ttccgtcgat tggatctaca atccagactt cgcgacaaga aagtcgatca
     1381 tcatcgtctg tggactcttc agaaagccag cccgcatccg gcagaaggcg tctaagatga
     1441 ctttctaaaa tgcgattgga ttcgaggtca gcagtggtga gaggccccgc gtcccctttg
     1501 ctggaaacgg agaatgattt gtcgttgaat actcgcagaa cggcggacgc ggcggttgcc
     1561 accgcgtcag ataggccgga caaaatgtcc gcgtcagtca cggagtcttt gctggctgcg
     1621 ccggagttgc aggagcagtt ggattcgtgc tggattcgcg atgggtcatg tccacttccg
     1681 ctgcatcgag gaatttctca gtgataggag actgagcaaa gctctccggg ttacgcgcat
     1741 actcaaaacg aaagaatgag ttcttgtatt tcacgaagta gaccagagga gaagtagggt
     1801 tttcgtattc gatggtctgg atggacaggt ctttgatgat cgtctccttt tcttcgtagt
     1861 agtgacgagt catggccatg cgaaccgcgt tcaccaggtt cgtttccaga gtcttaagtc
     1921 tggcttcagg gccggtctgc ttgttgcgaa tggcttcttc tactgcccgg tattcttgct
     1981 ggacgccttc ttttcttccg gcccagaccg gaacactgaa caatgcaatg attacaatta
     2041 cccgcttcat ctttacctcg atcggcaaca ggatggcgcg aattgagccg gaagcaacga
     2101 taatttccgg gtttattcta tcttctcgat gggagcaaac ctggccagga agtacctgac
     2161 cttctcatct ccatcaaact gtatgccgat ctttgcaccc gctactgtat cttcctgctc
     2221 caccacggtg cccgggccgt aggatttgtg tcgaattcta tccccgattt gtacgggcac
     2281 aacctgcact gacggaggcg gcggccttct tcccactcca gcaaaactgc ccatagaatc
     2341 tcgcgcacgc ggagtgttct gagctctcgt ggcgatccct tcttcgaaaa cggatacgtc
     2401 tatttctgaa atgaaacgag acggaagcct gggttgatag ctgccctgaa tgcggcgaaa
     2461 tcgactgcta gacaaataca gaaattcacg cgctcgagtg attcccacat acgcaagcct
     2521 gcgctcctct tctatgttgt cctcaagcga aaaagtatgc ggaatggttc cctcttccag
     2581 acccgtaaaa aacacagcgc gatattccag acctttcgcg ttgtgaaggg tgatcaggtt
     2641 gactgcgtct tgaccctcag catctccctg ctctgaatag agagagattt gctggagata
     2701 cgcactgaga tttggttcag attcgctttc ctcgaattcc tggatggagg aagcgaattc
     2761 ttctacgttt tcgatcctgg attcaccggg gttctcatct ttcgcaagga attccagata
     2821 tcctgtcttt tctatgatgg attcaagcac cagagaaggt cttgctggat tcatcccgcg
     2881 ccagttttca aaccaggaat gcaagtccat gatcgcgcgc gaaccacgca caccctggag
     2941 ttcccccgct ctcgccatag catcgtagag agtgattcct tctgtcacag aaagcgcttc
     3001 tagacgagcc aggcccgcct ctccaaaacc acgggccgga gtattgatga tgcgcatcaa
     3061 gctgatgctg tcttccggat tcacaaccac ggacagatag gcgattaaat ctttgatttc
     3121 ctttctgtcg tagaagggaa agcctcccac gagcaaatac ggaatgccat ctcgtcttag
     3181 caattcttca aagtgtctgg attgcgcgtt ggtgcgataa aacaccgcgg actccgcata
     3241 tggaattccc tgctgcttga gactcttgat acgcgaaatg gctcccgccg cttcttctgt
     3301 gtcagactcg taaactgtgt aatgaatttt ctctccacct ggctggcggg taaagagagt
     3361 cttttcgcga cgctcactgt tgtgcgcgat gagagaggaa gctgctttga gtatgggcgc
     3421 agtgcttcta taattttctt ccagacgaag cactgtgcaa tttggataat ctttttcaaa
     3481 cgaaagaata ttctcaatat ccgcaccacg ccaggaatag atggactgat catcgtcccc
     3541 gacgactgct atgtttgtgt gatcgcgcgc gatcaatcgc ccaagcatgt actgtgcgtg
     3601 attcgtatcc tgatactcat cgatcatgaa atatcgccac atgctctggt agtctgcgag
     3661 cacttctggc tctctttcga acatgcgcac tgtctcgtag atcaaatcgc caaaatcaag
     3721 agcattgttt tttctcagac ggatcatgta ctcgcgataa atcggaatca cgtcctcttc
     3781 atacctgtcc gccgtttgct caaacttttc aggagaaacc atctgatcgc gtgcgcgatt
     3841 gatcctttct gcagcagcgc gcggagtgag ccacgacgaa gtgatgtcta gatccttgat
     3901 gactctcttg atcaatgctt cctgatcatt ctgatcatag atagaaaagc ctgacttcag
     3961 tcctagttta ggcgcgttcc ttcgcagaat gtaaagccca agagagtgaa acgtgcgaac
     4021 cagtacactg tccgccatgg gaccaatcaa accgtagagc ctctggtgca tttctcccgc
     4081 agctttgttt gtaaaggtaa cggcgcacac tctgtacggc ggaacattca gtacgcgaca
     4141 cagataggcg attctgtgag tgatgactcg cgtctttccg gatccggcgc cggctagaat
     4201 cagaagagga ccttcattgt gcaacaccgc ctgcttctga accggattca gattggccaa
     4261 aagctcctct tctatctttg cctttaattc tgttttcaaa aatgatacac tccgagcttt
     4321 tcttcgcccg catgataact ggccatgggc cttgcctgaa gaagcgcaga cggaacgcgg
     4381 actgagtccg gtcctgcctc cagagtccct gacctttcca gatgatacaa acgatcccga
     4441 ctgcctagac caggcatgat cgcgtgaaac aatccagcac cgccttccac cagaacttca
     4501 cccagaccgc gatctgcgag cacatcgcga agttctatca tgaaagatgg gtgagacaga
     4561 gggggaagag cgtgcgtcca cctgcccggc catggttcta cggacaaaaa gagaggttct
     4621 ctcccggttg tttctgtaag tgccttctgt ttttcaaacc aggtctctgc tcctggaaaa
     4681 ggacgaccca gaacaaatac gcgaagcggc tgattggaat tcgtagccgc cagttcgtca
     4741 tttgcgatga ttgcgtctac aaacggatcc tgcccggagg ttcgtttcat tctcaaagga
     4801 gatcttgttc gcgcctcaag ccctgggcga tcgacaaaaa tagtgcgcgg accaaccaga
     4861 acggcgtcca gcttcgcacg aagcatctga ccaaacgcaa ttccatcctc ggaggacacc
     4921 atgattctct tttctttgct tcccatgatc ccatcgcaac tggctacctt gagatgaaac
     4981 cgcggtccct gcccgagagt gcgaccaatg aatccttcga ggaattcgcg acccattccg
     5041 ttagaatcat ctacgatttg cagacctgat tcgcggagac gagcaaggcc ctctccgctc
     5101 aaactcggat ctagattccc aatgtgaagt ctcaggtttg gatagtttgc cagtcgtttc
     5161 gtgcacggag gagtgcgccc aaatttcgag catggttcga gagttacata cagatctcca
     5221 tctgcatcgc gcgggagact ttgatcaaat aggtcgagag caacaatttc cgcgtgccga
     5281 cgccctggaa tttctgttgc accggaaaaa atttgtcctt ttgactgaat gacacaggcg
     5341 acggacggat tgggccaggt gcggcccaga gcgatcacag attgccggct cagctctgca
     5401 actatgccgg catccacttt tctaccgccg aatacgagcg acgaattctt cgtagtactg
     5461 aacgctgtgc gcggccacga aaatcgaaga atacgttccc ataaaaatac caaagatcag
     5521 aacaagagca aaatccctga ggctgtccgc gccaccgagt agaagggcca gaacggagat
     5581 catagtcaga atggaagtaa ccagagttcg agagagagtc tgagtcattg cctcgtccat
     5641 ggttgtgcga agagacatct gcgctctgtc ttttgaattc tcgcggattc tatcgaagat
     5701 cacaatcgta tcattgatcg aataacctac gatggtaaga accgcggcaa cgactggaat
     5761 gcttggctca attcttgcca ctccaatgaa tccaatggtt agaatcacgt cgtgaacgag
     5821 tgaaagcgat gcgcccaatg caaa
//
LOCUS       DAOQJL010000204         5821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7856_length_5821_cov_4.067118, whole genome shotgun sequence.
ACCESSION   DAOQJL010000204 DAOQJL010000000
VERSION     DAOQJL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_5821_cov_4.067118"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..542
                     /locus_tag="PLG78_10090"
     CDS             177..542
                     /locus_tag="PLG78_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23509.1"
                     /translation="MSEPKVFSSQALTIKVREDGPGFILEWTGKSNEREPGKFITPIL
                     TEIMEKAGQNPVVLDFGNLEYMNSSTITPIIRILDTAKKNKQKIKILYKKALKWQDLS
                     FSALTIFKTEDGQISIEGV"
     gene            548..1939
                     /locus_tag="PLG78_10095"
     CDS             548..1939
                     /locus_tag="PLG78_10095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE23510.1"
                     /translation="MSAATTFEGELIGKERRIEVTVGYGSRYSLLATIQNNQLIPYNE
                     QFDELSLKANGSNISFGPCFLITDDNIGKFNGRIVFAHDAYNFDSFFQNTAAPGLEGH
                     FHNLPLIRSQKDRIEPLFKEYTQNLLYDLNVHKTFFDDLDRKLQLETDSVRRAAQNAI
                     IETEGRKFLRFFDQSIDDLQKIVKNFSKEENERHGFYFRRMVWHLILTSDFLARTNIK
                     PRGYQGDSEMMRMTYVNDYEGSSIFGKLLHKHPIETAAAQAVRNRRKYIPEILHETLG
                     HFPKTGKHAFRIMSIACGPAWEMQDVFAGSDSFDRFHLTLLDQDVEALGEAASAISAV
                     EKARGLKTRVDYLNESVRTMLRTPKLQDKWGQHHFIYSMGLFDYLTGPVAKAVIKKIF
                     ELLKPGGTMIIGNYHVDNPTKTYMDYWMDWVLFYRTETEILSLLADEPKAEASVTFED
                     SRSQMFLHVRKKA"
     gene            1936..3282
                     /locus_tag="PLG78_10100"
     CDS             1936..3282
                     /locus_tag="PLG78_10100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23511.1"
                     /translation="MKFLKRFIVPNPQLTPEHEALFHEETLSSARNSLQFVCFAAGTL
                     YPIFGVLDYISFPDRYLGLVFIRCIVVPFVFLTLGLTFVDRLRPYQNWFGIGLFQVLA
                     LGIIAMTWFTGGKTSPYYAGIILVLLTQIVAMPWRLRMVFANSFGVWGIYTISVILLD
                     WINEANSSEADWRLFIGNNFFLIGTILIGSVWSGISFRLRKVVFLNRAQLALEKEKSE
                     ELLRNTLPDFVIEELKRNGSVHPRRLDCTVLFTDFAGFTKISEKMEPEDVLLELDRSF
                     EYFDTICEKFGMDKIKTIGDSYMCAAGVDAAGKRHAIVAALTAIEFRSYMNMMKQIKM
                     AVNEEYWQIRIGIHSGPVVGGIVGKFRYAYDIWGDTVNTASRMESSGIIGEINISQDT
                     NSLLQEYFVTHSRGLVNAKGKGELEMFTLDSIRPEFADDEAGRTPNAKLLSLIDIS"
     gene            3325..4665
                     /locus_tag="PLG78_10105"
     CDS             3325..4665
                     /locus_tag="PLG78_10105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE23512.1"
                     /translation="MSTPAADVNSITEFRPYLNAIIHSWLKIMTALGYTLVPLFFVLD
                     IFVIPKEHSDLLPRFAIYRAIVTAFIIAQSIVIHRTKPSNYSFIHGYVFTVSVSMVIV
                     MMTRDLGGFNAPYYAGLNLVIIAANMLMPWHAKHSAINGLLTVGMYVLVNSFFGGPFT
                     WNILINNLYFLFGTVIIVAAINFVKHNLIKKEFQGRQELRLARDALWGEMEIAKRIQT
                     ALLPPDHGISGYEIAAVMVPADEVGGDYYDILDFGDKKWITIGDVSGHGVESGLIMMM
                     TQTSVKSMVSNDPMASPAAVLSHVNTVLKENISRLGADRYMTISALSLNGKTMVVAGK
                     HQDIFIYRKKSRHIETVHTHGAWIGILDDITDFLTDQEVALDVGDIILLFTDGITEAE
                     NKEGDMFGEERLNDKLNEYANLPVKEIMNNILRDVTVFQERQADDITLLIIKKI"
     gene            complement(4732..5118)
                     /locus_tag="PLG78_10110"
     CDS             complement(4732..5118)
                     /locus_tag="PLG78_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23513.1"
                     /translation="MIEKNLQDVLNAGIGLFKSSEENFKVAVSNLEKTFNDMRNKGAA
                     DNSDAAIKIREILENTLRSAKDVQAKAEQNLNVFMAEAQKNYSQVLEQVKKVVGEERI
                     RDLNTKIDELTTLVKQKVGQPGGPKV"
     gene            5328..5675
                     /locus_tag="PLG78_10115"
     CDS             5328..5675
                     /locus_tag="PLG78_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNE23514.1"
                     /translation="MDKAKREKFKEKLLKAKAEIIKELEVEQEYFVYNDQGDLVDVAD
                     SVISNEILNKLSDMDLDKVRLIDIALEKMEKGTYGVCEGTGKNIPEARLNAIPWTRYT
                     VEYAEQMEKLKAN"
BASE COUNT         1489 a         1375 c         1466 g         1491 t
ORIGIN      
        1 gatggcgcgt ctccgcctgc cgccgccggg gcggctcgcc gcgggagaga gcggactagg
       61 gctagtgcga atcgcatacg aaggtcagtc gattctggac ttctacgtgg atgatcacaa
      121 catactcgcc gtatccgcgg tgcttgagta caaatagata gtggggagct ggattcatga
      181 gtgaacctaa agtttttagc agccaggcgc tgaccatcaa agtgcgcgaa gatggtcccg
      241 gattcattct ggagtggaca gggaaaagca acgaaagaga gccagggaaa ttcatcacgc
      301 ccatcctcac tgaaatcatg gagaaggcgg ggcagaaccc tgtggttctc gatttcggaa
      361 atctggaata tatgaattcg tccaccatca ctccgatcat tcggatcctg gacactgcga
      421 agaagaacaa gcagaagatc aagattctct acaaaaaggc gctcaagtgg caggacctga
      481 gcttcagcgc gctgaccatt ttcaaaactg aagacggtca gatctctatt gagggtgtgt
      541 aaggtcaatg tccgctgcaa caaccttcga aggcgaattg attggcaaag aacgccgcat
      601 tgaagtcacc gtgggctatg gctcccggta ttcgttgctt gctaccattc agaataacca
      661 gctcatcccg tacaacgaac aattcgatga gttgagcctg aaggcgaacg gcagcaatat
      721 atcattcggt ccgtgctttc tgatcacgga tgacaacatt ggaaaattca acggcaggat
      781 tgtattcgca cacgatgcgt acaactttga ttcttttttc cagaacactg cggctcccgg
      841 cctcgaaggt cattttcaca atcttcctct gattcgttcg cagaaagatc gaattgaacc
      901 tctcttcaaa gaatatactc agaacttgct ctatgatttg aacgtgcata aaacgttctt
      961 tgatgatctc gatcgtaagc ttcagcttga aactgattcc gtcaggcgcg cggcgcaaaa
     1021 tgcaatcata gaaacagaag ggcgcaagtt cctgcgtttc tttgatcaaa gcattgatga
     1081 tctacagaag atcgtgaaga atttttccaa agaagagaat gagcggcatg gcttctattt
     1141 tcgccgcatg gtctggcacc tgattttgac gtctgacttt ctggctcgaa ccaacatcaa
     1201 acctcgtgga taccagggcg attcagagat gatgcgcatg acttatgtca atgactacga
     1261 aggcagctcc atttttggca aactcctgca caagcacccg attgagacgg cagctgcgca
     1321 ggcagtgcgc aatcggcgta aatatatacc tgaaattctc catgaaactc tgggacattt
     1381 cccaaaaacc gggaagcacg cttttagaat catgtcgatt gcatgcggcc ctgcctggga
     1441 aatgcaggat gttttcgctg gatcagacag ctttgaccgt tttcacctga ctctcctgga
     1501 tcaggatgtg gaagctctcg gagaagcggc ctctgccatt tctgctgtgg agaaagctcg
     1561 aggtctcaag actcgcgtgg attatttgaa tgaatctgtg cgaaccatgc ttcgcacgcc
     1621 aaagcttcag gacaaatggg gccagcatca tttcatctat tctatgggcc tctttgacta
     1681 cttgaccggc cctgtggcaa aagcggtgat caagaagatc ttcgaattgc tgaagccagg
     1741 cgggaccatg atcattggga actaccatgt ggacaatcca accaaaacct acatggacta
     1801 ctggatggac tgggttctct tctatagaac ggaaactgaa attctatctt tgcttgcgga
     1861 tgagccaaaa gcggaagcat ccgtcacatt tgaagattca agaagtcaga tgtttctcca
     1921 tgttcgcaag aaagcatgaa gtttctcaaa agattcatcg ttccaaatcc acagcttacg
     1981 ccagagcatg aagctctttt tcacgaagag actctgagta gcgcgaggaa ttcccttcag
     2041 tttgtttgct ttgccgcggg aacgctctat cccattttcg gtgtactcga ctacatttca
     2101 tttcccgatc gatacctggg tctcgtattc attcgctgca tcgtagttcc gtttgttttt
     2161 ctgactcttg gtttaacgtt cgtcgatcgt cttcgccctt accagaactg gtttgggatt
     2221 ggactgtttc aggttctggc cctgggcatc attgcgatga cctggtttac gggtggaaaa
     2281 acaagtccat actatgcggg catcattctt gtccttctca ctcagatcgt cgccatgcca
     2341 tggagacttc gcatggtttt cgccaacagc tttggagtct ggggcattta tacgatttct
     2401 gtgatcttgc tagactggat caatgaagcc aattcgagcg aagcagactg gcggctcttc
     2461 attgggaaca acttcttttt gatcgggaca atcctcattg gatctgtctg gtccggcatc
     2521 agctttcgtc tgcgcaaggt tgtgtttctc aaccgagctc aacttgctct ggaaaaagaa
     2581 aaaagcgaag agcttctgcg aaatactctt ccagattttg tgatcgaaga gttgaagaga
     2641 aacggaagtg ttcatccaag aagactggat tgcactgtcc tgtttactga cttcgcgggt
     2701 ttcacaaaga tttcagagaa gatggaaccc gaagacgttc ttctcgaact cgatcggtcc
     2761 tttgaatact tcgataccat ctgtgaaaag tttggaatgg ataagatcaa gacgatcggg
     2821 gactcttata tgtgcgcggc cggtgtggat gcggcaggaa agcgccatgc gattgtggct
     2881 gccctcactg ccattgagtt tcgcagctac atgaatatga tgaagcagat caagatggcc
     2941 gtgaatgaag aatactggca aattcgaatc ggcattcatt ccggcccggt cgtggggggc
     3001 atcgtaggta agttccggta tgcctacgac atatgggggg atacggtgaa caccgcatcg
     3061 cgcatggaaa gctctggaat tataggtgaa atcaacattt cgcaggatac caactctctc
     3121 ctgcaggaat acttcgtaac ccactccagg gggctggtca atgcgaaggg gaagggggag
     3181 ctggagatgt tcaccctgga ctcgattcgc ccggaattcg cggacgacga agccggccga
     3241 acccccaatg cgaagctctt atcgttgatt gacatttcct gagagcccct tcatcctgcc
     3301 ctgcaggggg acgatatctg aaacatgagc acgccggcag ccgatgtgaa ctcgattacc
     3361 gagttcaggc cctatcttaa cgcaatcatc cattcgtggc tcaagatcat gactgcgctg
     3421 ggctacaccc tggtgcctct gttctttgtc ctggacatat ttgtcattcc caaagagcat
     3481 tcggaccttc tgccgcgctt tgcgatctat cgcgccattg tcacagcgtt catcatcgcg
     3541 caatcgatcg tcattcatcg cacaaagcct tctaactatt cgttcatcca tggctacgtc
     3601 tttactgtct ctgtcagtat ggtcatcgtg atgatgactc gcgacctggg cggtttcaat
     3661 gcgccgtatt acgcgggcct caatctggtc atcattgcag ccaacatgct catgccctgg
     3721 catgcaaagc attctgccat caacggactt ctgacagtgg gcatgtatgt gctggtcaat
     3781 tccttcttcg gtggcccgtt tacctggaac atattgataa acaatcttta ctttctcttt
     3841 gggacagtga tcattgtcgc cgcgatcaac ttcgtaaagc acaacctcat caagaaagag
     3901 tttcagggac gtcaggaact gcgtctggca agagatgcgt tgtggggaga aatggaaatc
     3961 gccaagcgaa tccagacggc actccttcct ccggatcatg gcatctcggg ctacgaaatt
     4021 gcagcagtca tggttcctgc agatgaagtg ggcggagact actacgacat tctcgacttc
     4081 ggggataaga agtggatcac aattggagac gtgtccggtc atggagtgga atcaggtctc
     4141 atcatgatga tgactcaaac cagtgtgaaa tccatggtga gcaatgatcc catggcaagt
     4201 ccggctgctg ttctcagtca cgtgaatact gttctcaagg agaacatatc gcgtctgggt
     4261 gcggatcgtt acatgacgat ttctgctctt tctttgaacg gaaagacaat ggtagtggca
     4321 ggaaagcatc aggacatttt catttatcgc aaaaagtcca ggcacattga gacagtacac
     4381 acacacggtg catggattgg aattctggac gacattactg atttccttac ggatcaggaa
     4441 gtggctctgg atgtgggaga tatcattcta cttttcacag atggaattac agaagcagaa
     4501 aacaaagaag gcgatatgtt cggcgaagaa cgtctgaacg acaagctgaa tgaatatgca
     4561 aaccttcctg tcaaagaaat catgaacaac attctgcgcg atgtaactgt tttccaggaa
     4621 cgtcaggcag acgatatcac tctgttgatc atcaagaaga tctagcaacc agcgggcaaa
     4681 aaaaagccgg gctattgccc ggctgctctt cgcattcgtt tagaacgcta attaaacttt
     4741 tggtccgcca ggctggccga ctttttgttt tacgagcgtt gtaagctcat cgatttttgt
     4801 gttgaggtcg cggattcttt cctcgcctac cactttcttt acctgctcga gaacctgact
     4861 gtagttcttc tgggcttctg ccatgaacac attgaggttc tgttctgcct tcgcctggac
     4921 gtcttttgcg ctacgcaggg tgttttcaag gatctcacgg atcttgatcg ctgcgtctga
     4981 gttatcagcg gcgcctttgt ttctcatatc attgaaggtt ttctccagat tggacactgc
     5041 cactttgaag ttctcttcgc tggatttgaa aaggccgatg ccggcgttca gtacgtcttg
     5101 aaggtttttt tcgatcatgg ttttctccga tttgccggac atccttggat gtccttatgc
     5161 agtgcacaat tgatttagtg cgccgcaaaa tcaagtaaaa aacgatcccg aggtgcattt
     5221 tttcggtacg ggagaggcca tccgggccca tgatcccttt tttctttaca gggagcccag
     5281 ggccggcagg attgtgcctt tactagttct ggtggacata attccgtatg gataaggcaa
     5341 agcgcgaaaa attcaaagag aagctgctca aggccaaggc tgagatcatc aaagagcttg
     5401 aggtagagca ggagtacttc gtatacaacg atcagggaga cctggtcgac gtggcggaca
     5461 gcgtcatcag caacgaaata ctaaataaac tttcagacat ggacctggac aaagttcggc
     5521 tcatagacat tgctcttgag aagatggaaa agggcacgta cggcgtttgt gaaggcaccg
     5581 gaaagaatat tcctgaagca agactcaatg cgattccatg gactcgctac acagtggaat
     5641 atgcagagca aatggagaaa ctgaaggcta attgatgtca aagcaggagc acggtaagta
     5701 cggcgaaagc cagatcacag cgctaaaagg tctagaagcg gtccgcaaaa gaccggggat
     5761 gtacatcgga acgcaggacg caactggcct gcacaagatg gtctacgaag ttgtagataa
     5821 c
//
LOCUS       DAOQJL010000205         5811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7882_length_5811_cov_3.638985, whole genome shotgun sequence.
ACCESSION   DAOQJL010000205 DAOQJL010000000
VERSION     DAOQJL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_5811_cov_3.638985"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLG78_10120"
     CDS             <1..779
                     /locus_tag="PLG78_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE23515.1"
                     /translation="WPPNVRPNYEAGGSAGDAWNLCCDLSRTGERRLVSAANEILKLL
                     EKQAGEIPESVRKSFIAGARELVVPSGAQIAFENEACARLPFVLSGGLRVYKAAPDGR
                     SITLYDIDPGETCVLTMSCILGGEPFPAGAEALGETRVLVVSSEDWLRWVESSPSLRR
                     YSHQIAARRLSAVIETLSEVAFHRVNQRVARLIVRRSTGSGMQATHQQIADELGTARE
                     VVSRILKEFERNGWIRMSRHEIVLLDKSALVKLAEDADAV"
     gene            852..1055
                     /locus_tag="PLG78_10125"
     CDS             852..1055
                     /locus_tag="PLG78_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE23516.1"
                     /translation="MKKNMGTIDRALRVVAALAVGVLWYLGVISGTVAIVLGVLAVVF
                     VLTSLISFCPLYLPFGLSTRGKE"
     gene            complement(1171..1572)
                     /locus_tag="PLG78_10130"
     CDS             complement(1171..1572)
                     /locus_tag="PLG78_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNE23517.1"
                     /translation="MQSEHDDKIPLDLVVQGAVFEYRRAFSRAEILAFADLSGDHGEH
                     HVHGDRLMAHGLLLASIVTKIGGDLNYISQEMLLRFEAPVYEGEEVTGRITLTGVIDK
                     RLRKKIEMECNLFKGDVPVVRGTSYGQILRK"
     gene            complement(1643..1990)
                     /locus_tag="PLG78_10135"
     CDS             complement(1643..1990)
                     /locus_tag="PLG78_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNE23518.1"
                     /translation="MSEISVAHGDVLFVVARAKALEVQEIAAALGKDKSTVSSIVSQL
                     EAHGYLARARDPGDARRAIIRLTPLARRITPRMLAISRVFNARLLTGISRQERKFLEE
                     LLRRMDTNLAELE"
     gene            2439..2798
                     /locus_tag="PLG78_10140"
     CDS             2439..2798
                     /locus_tag="PLG78_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23519.1"
                     /translation="MNFKRTLLSGLCLLLLGCATTATQQVDIPVYFGPRPGAPASKDF
                     NNIAARDSFLLVGVRNSAGADQKSEVEKVLINNTKSTKDWATIQEVDVSGYTIIILPW
                     MFGTAENSVKGKVEHAQ"
     gene            2851..3222
                     /locus_tag="PLG78_10145"
     CDS             2851..3222
                     /locus_tag="PLG78_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23520.1"
                     /translation="MVGGRGTATFDQVRYPISGTTTLLKKDGTTLNAASAGKPLGHVQ
                     AHKFYWAMLYGSVSLTGKYDLSQDMNAAVEEVGGNAVINAKLTNTQCPTNYVLVVLGI
                     LPFIPGCVSVTLEGDAVKLDL"
     gene            3236..3532
                     /locus_tag="PLG78_10150"
     CDS             3236..3532
                     /locus_tag="PLG78_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23521.1"
                     /translation="MLVLLLASCATPQAPVLTEEEKKIVVLQATGVKFLEMNCKPLSG
                     VEDYAFRESAIQAAHRRRATHVQLLPLASGMSSTGGFATSYQSFSYRAWSCISP"
     gene            complement(3561..3851)
                     /locus_tag="PLG78_10155"
     CDS             complement(3561..3851)
                     /locus_tag="PLG78_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23522.1"
                     /translation="MNPEEIRAAFDRIKRRNLFAGFVPLVFPFFAFAIYQKDLFKQPE
                     ILFVSILPALAAAVWVHLVNWRCPACRAWLGRFFTVSECPSCKVKLTLKLNT"
     gene            4107..>5811
                     /locus_tag="PLG78_10160"
     CDS             4107..>5811
                     /locus_tag="PLG78_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23523.1"
                     /translation="MKHFIRLTLLLYFVSALPAAPVHMTDEEVPVSGTSLQIIEDKLA
                     ALDLTNLPEGLWKSSHNGDFGAGFTEAVYWLKFSAVNMRPEENWTLHIENALVDELEI
                     WKTGGESAHIQTGTKIPYKDRELAGRLFHLPVKIKKGETANFLIRAKMRASMNLPIRL
                     RTDRVTASWNNRENLILGLFHGAVLIMIVYHAFIWLSVRDRIYAYFILVLLFGSTFMA
                     AQNGLAFQYLWPGIGERGVQINLTFGVLTLLTSSLFIREMLNLRARWLPGFFAANGLI
                     ILSAICLAAVWIVSYKIAASTMTTLTLMQAMVAMSVGLRFALRRDRIAAWFASAYFVV
                     IASSDLFALRSVGVIPPSQWITHSLMIGILMQNILLSLSLAERINVLHLEAEVSKQAS
                     ELKTKFMATMSHEIRTPMHGVIGLTNLLLDTQLDAEQRMLATQTSRSAQALLEIINDV
                     LDFSKIEAGRLELHPETTRITDPVASVVNLMQPLAQKKGLHIRTSLSREIPPFVSVDA
                     GRLRQILLNLVANAVKFTSEGEISLTGGYEQGNIFFSVRDTGIGIDSEYLPHLFDPFY
                     QV"
BASE COUNT         1412 a         1559 c         1485 g         1355 t
ORIGIN      
        1 attggccgcc taacgttagg ccaaactacg aggcaggagg tagcgcggga gacgcatgga
       61 atctttgttg cgatctgtcg cggaccggtg agcgtcgcct tgtgagcgcg gccaatgaaa
      121 ttctcaagct gcttgagaag caggccggag agatcccgga gtcagtgcga aagtccttta
      181 tcgcaggcgc gcgcgagctt gtggttccat ccggcgcgca aattgcgttt gaaaacgaag
      241 cctgcgcgcg tctgcctttt gtgctcagcg gagggcttcg ggtctacaag gcggcacccg
      301 acggcaggtc catcactcta tacgacatcg atcccgggga aacctgcgtc cttacgatga
      361 gctgcattct gggtggagaa ccattccctg ccggcgctga ggctctcgga gaaacgcgag
      421 tcctggtagt ctcgtccgaa gactggctgc gctgggtgga gagctctccc tctctgcgtc
      481 gttattcgca ccagattgcg gcacgacgat tgagcgccgt catcgaaaca ctcagcgagg
      541 ttgcttttca tcgggtcaac cagcgcgtgg cccgcttgat tgttcgccgc tccacaggca
      601 gcggtatgca ggcaacacat cagcagatcg cagatgagct cggcacagcc cgcgaagtgg
      661 tcagtcgcat cctgaaggaa tttgaacgca atggatggat ccgcatgtcc cggcatgaaa
      721 ttgtcctgct agacaaatcg gccctggtga agcttgcaga agatgccgac gctgtgtgat
      781 ctatgtcaca gacaggtcta gcatttcgtg ctatattcct acatgttcaa caagattgaa
      841 cggaggaata tatgaaaaag aatatgggaa ccatagaccg cgccttgcgt gtagtggctg
      901 cactggcagt aggcgtgctc tggtatctgg gcgtgatttc tggcacagtt gccatcgtgc
      961 tcggcgtcct ggcagttgta ttcgtgctta cgagcctgat cagcttctgc cctctctacc
     1021 ttccctttgg cctctccact cgcggcaaag aataattgcg agagcatgac tgccacgcgc
     1081 atcgccggcg tggcagtcat gccgccgcgc ggcataccgt taggcgattt atattggacc
     1141 acgcactctt cactacgcgc atgaatgaat tcattttcgc aagatctgtc cgtaactcgt
     1201 acctctcaca acgggaacat ctcctttaaa gaggttacat tccatttcga ttttctttcg
     1261 cagacgctta tcaatcactc cagtcagggt gatcctgcca gtcacctctt cgccctcgta
     1321 aacgggtgct tcaaatcgca gtagcatttc ctgggatata tagttcaaat ctccgccgat
     1381 ctttgtcacg atactcgcca acagaagtcc atgcgccatc aaccgatctc catgaacatg
     1441 atgttccccg tgatcgcccg aaaggtctgc aaacgccagt atttctgcgc gtgagaaagc
     1501 tcgccggtat tcaaaaacag ctccctgaac cacaagatcc agaggaattt tgtcatcatg
     1561 ttccgattgc attggcggct ccaatagttt tgtacaaaac cattttccgt gagtcacgct
     1621 cgtcaacaag ctttcttcgt ttctattcca gttccgcgag attggtatcc atacgccgca
     1681 gaagctcttc cagaaacttg cgttcctgcc tggaaatacc ggtcagaaga cgggcgttga
     1741 aaacccttga aatggcaagc atccgtggcg taatccgccg cgcgagcgga gtaaggcgaa
     1801 tgattgcacg ccgggcatcc cctggatcgc gcgccctggc cagatacccg tgtgcttcca
     1861 attgagaaac gatggaactg acggtggact tatcctttcc aagggccgcc gcgatctcct
     1921 gaacttctag agcttttgcg cgcgccacaa caaacaatac atcgccgtgc gcgacggaaa
     1981 tctcggacaa tcccgcttcc tccagatgct tttcaagata gcggaaagct gcggatcgaa
     2041 cccggctcag gaggtgcagt aaagaagttt tcaaatggta gctcgatcag tgaacaaaaa
     2101 caaagaacaa gacgagggct gtcagcttct ggtcatccag gaatcgctct gcattcaagc
     2161 tcgccactgc gaagctccgc gcaatcaaaa aagatccgct ccatgacgat atcaaggatg
     2221 ttgctttgaa agagatagtc cacctcccga ggtcgcgaga tgaatgcact aacattcgaa
     2281 tgaaagctac gtaaagcgta atgaacaaca caaagatcgt gtgcagaacg aatcagttaa
     2341 agaaaatcaa gtatacgcgc aggaggatcg agtctcttca aaaaatgatt gaccgcattg
     2401 gcgcctaacg gtcctttatg ccgcgggaga accagccaat gaacttcaaa cgaacgcttc
     2461 tttccgggct atgcctgctt ctactcggct gcgccactac agcaacgcag caggtcgaca
     2521 ttcccgtcta tttcggacca cgccccggtg cgcccgcatc caaagacttc aacaacatcg
     2581 ccgccagaga ttcttttcta ttggtaggcg tgcgaaattc agccggagca gaccagaaaa
     2641 gcgaagttga aaaagtactg atcaacaata cgaaatctac aaaagactgg gccaccattc
     2701 aagaggtgga tgtctccggt tacacaatta tcatcttacc gtggatgttt ggtactgcgg
     2761 aaaattctgt gaaaggaaag gtagagcatg cgcaatagaa tttcattttt tgtattcgtc
     2821 gcgatattcg cgtcggtttg cacttcctgc atggtcggcg ggcgaggtac cgcgactttc
     2881 gatcaggttc gctatccgat ttctggcact acaaccttac tgaaaaagga cggtaccacc
     2941 ttgaacgcgg cctccgccgg caagccactc gggcatgtac aggcacacaa gttttactgg
     3001 gccatgttgt acggatccgt ttcgctgacc ggcaaatatg acctcagcca ggatatgaat
     3061 gcggcggtag aagaagtggg cggcaatgca gtgatcaacg ccaaactcac gaacacacag
     3121 tgccctacca attatgtact ggtagtgctc ggaattctcc cgtttattcc gggttgtgtt
     3181 tccgtcacgc tggaaggcga cgcagtaaag cttgaccttt gattgcagac ggtgcatgct
     3241 cgttttgctg ctcgcgagct gcgcaacccc gcaggcgccc gttctaactg aagaagagaa
     3301 gaagattgta gtccttcagg cgacgggcgt aaaattcctg gaaatgaact gcaaaccgct
     3361 cagcggagtg gaggactatg catttcggga aagcgcaatc caggctgcgc atcgacgtcg
     3421 cgccacgcat gtgcagctcc tacctctggc aagcggaatg agttcgaccg gcggcttcgc
     3481 cacatcctat cagtcgtttt cataccgcgc atggtcctgt atttcgccct gacttctgtt
     3541 cctggttgcc cgcaggggtt tcaagtgttc aatttcaatg tcagcttcac tttgcaggaa
     3601 ggacactcgc tgactgtaaa gaagcgaccc agccacgccc tgcaagcggg acagcgccag
     3661 ttcaccagat gaacccagac cgccgcagca agtgcgggaa gaatggaaac gaacagaatc
     3721 tccggttgtt taaacaggtc tttctggtaa attgcaaatg cgaaaaatgg aaagaccagc
     3781 ggaacaaacc ccgcgaagag atttctcctt ttgattctat cgaacgcggc gcggatttct
     3841 tcagggttca tctgactcaa ggtccgcgat tgacggccgc gggcaactgc attatgaact
     3901 taaatatcgc acgaattaaa tgcggaccag agtatcactc gcctaccatc gagggaattg
     3961 ctccgctttc gcgctgttcg cggcgaattt ccgcgcctca aaaaagattg cgaggtgccg
     4021 cgagaaacta attatcggag tcaaacatgg gcatgtctgg aaaggcgcta taaattgagt
     4081 cgaaagccag ggccaatcgc atatccatga aacatttcat tcgcctaaca ttgctgttgt
     4141 atttcgtatc tgctttgcca gccgcgcctg tgcacatgac tgatgaagaa gtgccggtat
     4201 caggaacaag tcttcagatc atcgaagaca aactggctgc tctggacctg accaacttgc
     4261 cggagggcct ctggaaatcc tcccacaacg gagactttgg agccggcttc acagaagcag
     4321 tctactggct gaagttttcg gctgtgaata tgcgaccgga ggagaattgg acacttcata
     4381 ttgagaacgc actcgtagac gagcttgaga tctggaaaac gggcggcgaa tcggcgcaca
     4441 tccaaaccgg aaccaaaatc ccttacaaag accgagagct tgcaggccgg ctcttccatt
     4501 tgccggtcaa aatcaagaaa ggtgaaactg caaattttct cattcgcgcc aaaatgcggg
     4561 cgtcaatgaa tcttccgatc cgtcttcgca cggacagggt gactgcttcg tggaacaacc
     4621 gggagaattt aatactcggg cttttccacg gcgccgttct catcatgatc gtttaccatg
     4681 cattcatatg gttgtcggtg agagatagga tctatgccta cttcattcta gtattgctct
     4741 tcgggtccac tttcatggct gcgcaaaacg gcctcgcatt tcagtatctc tggcctggca
     4801 tcggcgaaag aggcgttcag ataaatctca cgttcggagt gctgacgctg ctcacgagca
     4861 gtctcttcat tagagaaatg ctcaatctac gtgcacgctg gcttccgggt tttttcgcgg
     4921 ccaacggact catcattttg agcgcaatct gtcttgctgc agtctggatc gtatcataca
     4981 aaattgccgc ttcgaccatg actaccttga ccttgatgca agccatggtg gccatgagtg
     5041 tggggctgcg cttcgcgctc cggcgcgaca ggattgcagc ctggtttgcc tctgcctatt
     5101 tcgttgtcat cgccagctct gatctttttg cgctccgaag tgtcggcgtg attccaccat
     5161 cccaatggat cactcattca ctcatgattg gcattctcat gcagaacatt ctactatcat
     5221 tgtctctggc cgagcggatc aatgtgctgc acctcgaggc cgaagtctca aagcaagcca
     5281 gtgaactcaa gactaagttc atggcaacta tgagccacga aattcgcact ccgatgcacg
     5341 gtgtgattgg actgacgaac ctcttgctcg atacacagct cgacgcagaa caacggatgc
     5401 tcgcaacgca aacatcgcga agcgctcaag cattgctcga gatcatcaat gacgttctag
     5461 atttttcgaa gatcgaagct ggcagacttg aactgcatcc agagactacc cggatcacgg
     5521 atcctgtcgc ctccgtcgtc aacctcatgc agcctctcgc acagaagaag ggcctgcaca
     5581 tacgcacgag cctcagtcgc gaaattccac catttgtgag cgtcgacgct ggacggctcc
     5641 ggcagatttt attgaatctt gtggcgaacg cagtgaagtt cacctctgaa ggcgaaatca
     5701 gtctgaccgg aggttacgag cagggaaata tcttcttcag cgttcgtgac acggggattg
     5761 ggattgattc ggaatacttg ccgcacttgt tcgacccatt ctaccaggta g
//
LOCUS       DAOQJL010000206         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7902_length_5805_cov_4.006783, whole genome shotgun sequence.
ACCESSION   DAOQJL010000206 DAOQJL010000000
VERSION     DAOQJL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7902_length_5805_cov_4.006783"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1006
                     /locus_tag="PLG78_10165"
     CDS             161..1006
                     /locus_tag="PLG78_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNE23524.1"
                     /translation="MRILVSALVLLVLSCSSAFNKRQSLYVGDHEAFYRLKSASYPSE
                     EIRAQLPKQTAFPDLDEDSIARLLATLKYRKAGLFGDTNRRIFYNEQLPEIAHRFKES
                     QARLDETQRMVLIVTHDPDNSVLSHLERTTLVYWADEAGLNIVLGEIRQDIPHNDMLE
                     RQDWTSVLPVSMKHSYSDLRLIDSPHYQLKQINGSTHATWAVFPMDQLAQLPAIPPDN
                     RVSLSKTEPASTEPAKPAKDNQPPAKSDPQLTRRLQDLKQALDQGLITQEEYDQKRKK
                     ILDSY"
     gene            complement(1000..1590)
                     /locus_tag="PLG78_10170"
     CDS             complement(1000..1590)
                     /locus_tag="PLG78_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23525.1"
                     /translation="MEVRANLFSLDKLRKSGQVSSLIVTFSMLVQTVFAFPGASDRML
                     ENFESVLWRAGADTYLTRVSAFDGSGHAMRVQFKSTSRLIPPRPIHLPAQSIALKLKV
                     FGSGRKDRLFVILKDAYGTDHSILIGNTDFKISKEMSIPLAGLTQAVHLEEKGLELRW
                     FLIVPASDKPASIQMDDLTVQTRDLIRIPPLAGSAQ"
     gene            1603..2613
                     /locus_tag="PLG78_10175"
     CDS             1603..2613
                     /locus_tag="PLG78_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23526.1"
                     /translation="MKRLCAILAIGFLTSVAYAQAPATTAPATEPKPATTQPGLNPGE
                     EPAKTTPAQPAQATGNGIWTSPAGENYGTSRVRFVLQAQDNLSGLDYIEYRVDTSEFR
                     RYVAPLQLDKEGPHTIVYHGVDKAGNREVDQVYHVVTDNKAPDVSILAAKPFVVKNGR
                     SFSSPNNSFTMRISDDFSGVKSVVYGVNSDEMKAYQDEVIKLTTPGSQLIQYTADDNL
                     GNRAQGSLLIEVDGAKPTVEIVPGEPLMPLGDKMYAKRRTGFKINGTDNGSGVEQILI
                     RVDGAQEWTTYSNVLYFDTEKEHSIEAKAIDAVGNESDVRKITFIVDDNPPTTDLRAN
                     VE"
     gene            2720..3388
                     /locus_tag="PLG78_10180"
     CDS             2720..3388
                     /locus_tag="PLG78_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23527.1"
                     /translation="MILDFLFPERCVVCSIEGQSLCGSHSLSSYVIDPVNRCPVCFVH
                     RGPQGCPKCAGQDIYFSYHSSIYDYRGAVRDLIHRWKFENERSVFKVFLPELTRRLGR
                     MNADRIGVIGSDLRTRSFAHSLDLLRAGKGAGLCGGVDFIKKRIGKKHKQSQRNQAGR
                     YFEIRGSLECVRDLSLVKHYVLLDDVFTTGATANEAARMARIAGAEKVSVLTVAMRED
                     LEHV"
     gene            3385..3729
                     /locus_tag="PLG78_10185"
     CDS             3385..3729
                     /locus_tag="PLG78_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE23528.1"
                     /translation="MIEIQKQGGPNVQILIISGKLEPDDAEVFQAELMELTSREKAAI
                     VLDMVGLNYICSTALGILISQHRRMRRLEGELKLVVEEGYVRNLLRMTMLDRVFPMFT
                     SREDAVRTFAAI"
     gene            3729..4334
                     /gene="rdgB"
                     /locus_tag="PLG78_10190"
     CDS             3729..4334
                     /gene="rdgB"
                     /locus_tag="PLG78_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNE23529.1"
                     /translation="MLALATGNRHKLEELTRQIGIDLLPPDRFGLVMDVEETGTTFEA
                     NAKLKADWLSAHSHIPALADDSGIVVDALGGEPGVYSARYGGDGKSDRDRRLLLLHNM
                     EGKQIRTARFVCVLCLSFPEGESVFFRGECEGTITFAEQGEGGFGYDPVFLDPESGLT
                     FAELDSAAKDRVSHRGKAIRKLIAWLEDGGRERVGSYSIPA"
     gene            complement(4338..5618)
                     /locus_tag="PLG78_10195"
     CDS             complement(4338..5618)
                     /locus_tag="PLG78_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNE23530.1"
                     /translation="MIQISTILAGARRIVQTQAALAGAGLSWLFGDRPPAPEFLRKTF
                     ERLGTTYIKLGQFIASSPSLFPEEYVREFQKCLDQTAPVPFAVVRGIIEEEFKDPDSI
                     FGFIDPVPLASASIAQVHTGKLRTGEEVVLKIQKPGVQDVMLADLTFLYAATRVLEWL
                     IPNFKRMSLSGILEDISTTILQECDFALEASHQKKFAEFLSRMDLENVATTPALYPHA
                     SSRRVLTMERFRGVALTDLESIRRVTSDPEETLIQALNVWMSSLMNNEFFHADLHAGN
                     LMVREDGRLGFIDFGIVGRIQKSTWKSMNHLMIALNTRNYKLAADSLIGIGAADEKAD
                     KKRFGEELKALFENMDQFAATVQASDGMLIDQGRVQEILLQIAKIGERNGIKFPREFA
                     LLIKQFLYFDRYIRLLAPDLDMMMDPRVEKAITD"
BASE COUNT         1465 a         1455 c         1488 g         1397 t
ORIGIN      
        1 aagtgaagaa aaggggcggt tccttcttca tatgtggcct gaataaatct atgcaatctg
       61 tctttcgcct ggccgggctc cacaagatct ttgaactcct ggagcaggaa caggcaaaca
      121 tgcggtttcc cgagctccag tcctagccga tactataacc atgcgaattc ttgtctctgc
      181 cctggtcctt ctggtcctat cctgctcatc tgctttcaat aaaagacagt ctctttatgt
      241 aggggatcat gaagcttttt acaggctgaa gtctgcctcc tatccatcag aggagattcg
      301 cgcacaactt ccgaaacaga ccgctttccc tgaccttgat gaggacagca ttgcgaggct
      361 cctggccact ctcaaatatc gcaaggcggg tctattcgga gacacaaaca gaagaatctt
      421 ttacaatgaa caactccctg agattgcaca cagattcaaa gaatcccagg ccaggctaga
      481 cgaaactcag agaatggttt tgatcgtgac tcacgatcct gacaacagcg ttctgagcca
      541 tctggaaaga acgactctgg tttactgggc ggacgaagca ggtttgaaca tcgtgctcgg
      601 cgaaatccgt caggacattc ctcacaatga catgctggaa agacaggact ggacgagtgt
      661 tcttcccgtc agtatgaagc attcctacag cgacctgcgc ttgatcgact ctccccacta
      721 ccagctcaag cagatcaacg gatccactca tgctacctgg gccgtgttcc ccatggatca
      781 actggcgcag ttgcctgcga tcccgcctga caatcgagta tccctttcaa aaacagagcc
      841 cgcgtcaacc gagccggcca aacctgccaa agacaatcag cctccggcga aaagcgatcc
      901 gcagcttacg aggagactgc aggatctcaa gcaggctctc gatcaggggc tgatcactca
      961 ggaagagtac gaccagaaaa gaaagaagat cctcgatagc tactgagcgg atcctgcgag
     1021 aggcggaatc cggatcaggt ccctggtctg cactgtaaga tcgtccatct gaatgctggc
     1081 cggcttgtct gaagctggaa caatcaagaa ccacctgagc tccaggcctt tttcttccag
     1141 atgaactgcc tgggtcaggc ccgcgagagg gatcgacatt tctttgctga ttttgaaatc
     1201 cgtatttcca atcaggatcg aatgatcggt gccatacgca tctttgagaa ttacaaacag
     1261 tctatctttg cgtcctgaac cgaatacttt tagctttaga gcaatgctct gggccggcag
     1321 gtggattggc ctggggggaa tgaggcgcga tgtgctttta aactgaacgc gcatggcatg
     1381 tccggacccg tcaaacgcgc tgacccgggt gaggtaggta tccgcgccgg cccgccacag
     1441 tacggactca aaattttcca gcatccgatc gcttgcgcct ggaaaagcga aaacagtctg
     1501 gacgagcata gaaaaagtga cgatcagact tgacacctgg cccgattttc ggagtttatc
     1561 caggctaaat agatttgccc gcacttccac gaggagaaat tcatgaaacg tctttgcgca
     1621 atcctggcaa tcggttttct tacatctgtt gcctacgcac aggcgccggc cactactgca
     1681 ccggcaactg aaccaaaacc agccaccact cagcctggac tgaatccagg agaggagcca
     1741 gccaaaacca ctccagctca gcctgcacag gccactggca atggaatctg gaccagcccg
     1801 gcaggagaga actacggaac tagccgagtt cgctttgttc tgcaagctca ggacaacctt
     1861 tccggtctcg attacatcga atacagagta gatacttctg aattccgtcg ctatgttgct
     1921 ccgctgcaac tcgacaaaga aggacctcat acaatcgttt atcatggcgt tgacaaagca
     1981 gggaaccgtg aagtagacca ggtttaccac gttgttacag acaacaaggc gcctgatgtt
     2041 tctatcctcg cagccaagcc attcgtcgta aaaaacggcc gcagcttcag ctctccaaac
     2101 aactctttca caatgagaat ttcagacgat ttctcaggcg tgaaatcagt agtatacgga
     2161 gtaaacagcg acgagatgaa agcataccag gatgaagtga tcaaacttac cactccaggt
     2221 tctcagctca tccagtacac tgcagacgac aatctcggaa atcgcgcaca gggatcgctt
     2281 ctcattgaag tagatggcgc aaagccaact gttgaaatcg ttccaggtga acctctgatg
     2341 cctcttggag acaagatgta tgcaaagcgt cgcacaggat tcaagatcaa tggaacagac
     2401 aacggttccg gcgttgagca aatcctgatt cgtgtagacg gagcccagga atggactaca
     2461 tacagcaacg ttctctactt cgacactgag aaagagcata gcattgaagc aaaagcaatc
     2521 gatgcagtag gaaacgaaag cgatgttcgc aagattacgt tcatcgtaga cgacaatccg
     2581 cctacaactg atcttcgtgc taacgttgag taactaaaca gtctcctcgg aaataaaagc
     2641 cggcttcacc gccggctttt tttttgtcct gtcgcgctct ccctcggaac gagattcaca
     2701 ggctctaccc ccagccagta tgatactgga ttttctgttt cctgagcgct gtgttgtttg
     2761 tagcatagaa ggccagagtc tctgcgggag ccactctctc tctagctacg tcatcgatcc
     2821 tgtgaatcgt tgtcccgtgt gcttcgtgca tcgaggtccg cagggttgtc caaaatgtgc
     2881 ggggcaggat atttacttct cttatcattc atcgatctat gattacaggg gtgccgtccg
     2941 ggacctcatt cacagatgga aattcgaaaa cgagcgttct gttttcaaag tgtttcttcc
     3001 agaattgacc cggcgcctgg ggcgaatgaa cgcagaccgc attggtgtga ttggttcgga
     3061 tcttcgcact cgctctttcg cgcactccct ggatctactc cgggcaggca agggggcggg
     3121 actttgcggt ggcgtggact tcattaaaaa aaggattggc aaaaagcaca aacaatcgca
     3181 gagaaatcaa gcgggccgat atttcgagat tcgcggttct ctggagtgcg tccgtgacct
     3241 gtccctcgta aaacattatg tcttgctgga tgacgtgttt acaacggggg ccacagcgaa
     3301 tgaagcggcc agaatggcac ggattgcagg tgcagagaaa gtatctgttc tgactgtggc
     3361 catgagagaa gatttggagc atgtatgatt gagattcaga aacagggcgg gcccaatgta
     3421 cagatcctga tcatctctgg caaattggag ccggatgacg cggaagtatt tcaggctgaa
     3481 ctcatggagc tgacaagccg cgaaaaggca gcgatcgtcc tggatatggt tggattgaat
     3541 tatatttgtt ctactgccct tgggattcta atttcgcagc atcgtagaat gcgaagactg
     3601 gaaggggaac tcaagctcgt tgtggaagaa ggctatgttc gcaatctact tcgcatgacc
     3661 atgctcgacc gcgtattccc catgtttact tcccgcgaag acgcagtgcg cacttttgct
     3721 gcgatctaat gcttgcactg gccacgggca atcgtcataa attagaagag ctcacccgcc
     3781 agatcggaat cgatcttctt ccaccggaca ggtttggcct ggtcatggac gtagaagaga
     3841 ccggaaccac atttgaagca aacgcaaaac taaaagcgga ctggctttcc gctcattccc
     3901 atattccagc tctggcagat gactcgggca ttgtagtaga tgcactcggc ggtgaacccg
     3961 gagtttatag cgctcgatat ggcggagacg gtaagtcgga tcgtgacagg agactgcttc
     4021 ttcttcataa catggaaggc aaacagattc gaactgctcg ttttgtttgc gtgctctgtt
     4081 tgtcttttcc ggaaggggaa tccgttttct ttagaggaga atgcgaaggc acaatcacat
     4141 tcgccgaaca gggcgaaggt ggttttggct atgacccggt gttcctggat ccggaaagcg
     4201 ggcttacgtt cgcagagctg gactcggcgg caaaagatcg ggtgagtcac cgcggaaaag
     4261 caattcgaaa attgatcgca tggcttgagg atggcggcag agagcgtgtt ggatcgtact
     4321 cgatccctgc ctgaggttca gtctgtgatt gctttttcta cacgcggatc catcatcatg
     4381 tccaggtcag gtgcaagcag tcgaatgtac ctatcgaaat ataggaactg tttgatcaag
     4441 agagcgaatt cgcgcggaaa tttgattccg tttctttccc ctatctttgc gatctgcaaa
     4501 agaatttctt gaacccttcc ttgatcgatc agcattccat ctgatgcttg cactgtggcc
     4561 gcaaattgat ccatgttttc gaagagcgcc ttgagctctt ctccaaatct tttcttgtcc
     4621 gccttctcat ctgcggcccc gattccaatc aagctgtctg ctgcgagttt gtagtttctg
     4681 gtattgagtg caatcatcag atgattcatg gacttccagg tggacttctg aattcggccc
     4741 acaatcccga agtcaatgaa tccgagtctg ccgtcctctc gaaccatgag gttgcccgcg
     4801 tgaaggtctg cgtgaaagaa ttcattgttc ataaggctgg acatccacac attcagtgcc
     4861 tgaatgagag tttcttccgg atcagaagtg acgcgtcgga tgctttctag atctgtaagt
     4921 gcaactcctc tgaatcgttc catggtgagc actcttctag aagaagcgtg cggatacagc
     4981 gcaggagttg tggcaacatt ctccaggtcc atgcgagaaa ggaattctgc gaatttcttc
     5041 tgatgcgaag cttcaagagc gaaatcgcac tcctggagaa tcgtcgtaga aatatcttct
     5101 agaatccctg agagactcat ccgcttgaaa tttggaatca accattcgag tactcgagtc
     5161 gcagcgtaga gaaaggtgag gtctgcgagc attacatcct ggactccagg cttttgaatc
     5221 ttcagtacta cttcttctcc cgtgcgaagc ttgcctgtat gaacctgagc gatggacgcg
     5281 gatgccagag gaaccgggtc gatgaatcca aaaatgctat caggatcctt gaattcctct
     5341 tcgatgattc cccgcaccac ggcgaaggga acgggagcag tctgatccag gcatttttgg
     5401 aattcgcgca catactcttc tggaaaaaga gagggagaac tggcaatgaa ttgacccagt
     5461 ttgatgtagg ttgtaccaag ccgttcgaat gttttgcgca agaattcggg tgccggcggt
     5521 ctgtctccga aaagccagga caatcctgcg ccggccaggg ccgcctgggt ctggacgatg
     5581 cggcgcgcac ccgccagaat agtactgatc tggatcatag atccatttag aagatgagtt
     5641 gcggtgatgg aagcgttttg tgaatggtcg gctgcacgcg cgctctctga aagaatagaa
     5701 tgggttggcc ccctgcggta tctatgccat agacctggtt tagaaagcga aggagggaag
     5761 gaaggtctcc ggtcgtcatg agctgaatca gattggccgg tctct
//
LOCUS       DAOQJL010000207         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7919_length_5797_cov_4.518635, whole genome shotgun sequence.
ACCESSION   DAOQJL010000207 DAOQJL010000000
VERSION     DAOQJL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7919_length_5797_cov_4.518635"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLG78_10200"
     CDS             complement(<1..850)
                     /locus_tag="PLG78_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23531.1"
                     /translation="MIRQTTLGVLLAFGMILLPLGAIQSQPMQMPGQQGPMARGPVVI
                     QGSVTNGTTGVAVAADEIALLRPGQGMQVLETIKPSSSVFKFAPVEAGNGPLLVRATF
                     QGENYIQVIPPTARATGSFQKVMVYETGAKPSDIMVTSGMQVIKKKETLHISRVYALE
                     NRSNPPRSYRGNDLKIFIPSGATDVQNQLQHQGSMPLPNVLGVDGVLSRSLRPGSAEL
                     TIEYDVEGTVLEERPHQLAGSPAVHPFIVLLHQPADAKPVFQGGEADQVEIPDLGPAL
                     RVRFTAD"
     gene            complement(847..1215)
                     /locus_tag="PLG78_10205"
     CDS             complement(847..1215)
                     /locus_tag="PLG78_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23532.1"
                     /translation="MSILLTIALVGCILALFFPLLRSRSAPEYYVGAPQDERIAETRA
                     QLQSVLAELRQDFDAGKLSEEEFSQMATPLANRLDSLASIPAPSRKKAISSNRQSCAV
                     CGYLGAPFEMCPQCGTEVKQ"
     gene            complement(1212..3719)
                     /gene="ccsA"
                     /locus_tag="PLG78_10210"
     CDS             complement(1212..3719)
                     /gene="ccsA"
                     /locus_tag="PLG78_10210"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNE23533.1"
                     /translation="MNLNQIGAAFLLLSAALVTFSIFMSLLGMSIRQIRSLIRFLPFE
                     LADDFRKLRGRRRLSHRMSHAVVSVWMVVRRRLIALLGFRSASVALDLARSALYVNAA
                     AASFAMFTLMVLMVQGDLTNHYVVTHSSADLHIFYLITGAWAGSSGSLLFWYFILTSL
                     SAVAVYQSRILRNRMPFLFLVLAGLQFIFVFMGLYFSDAQPFRVFPQPMKAGQGLNPL
                     LQHWGMIIHPPILYVGYVSSAIPFAIAMSVLVSRNTRNAFYPLLRRWTIFSWFFLGTG
                     ILLGSKWAYEELGWGGYWAWDPVENASLMPFLLMTAFLHTAIVSEQRGLLKFWTMLLI
                     LLAYHFSMLGTWITRSGVLQGPHSFSESSIGKPLIIYIGVSFYFYLRYIIMERKRLRP
                     DEELQTITSKEGSMLLNNFLMLLSVFVILIGVFSPLIPLDCRFDDGFSCSKAEWKLTA
                     YNRIMIPVGVLTLFLMGASPLFAWKKSADQVYVKNLRIPLILGVVVTIAFALLYGKMF
                     KPSGEDSAWGSSLVAEVFSILTVGIAVFTIAGIVQEYSRGIRSRRVRLGENSVQAFLH
                     LIGRNKRRYGGYLVHLSVVFLFLGYAGGSFKKTVKMDFMYYRMPQYPGSSEVHYYSGD
                     KSYVDNYEVEAGDLFFAPYFLANADQNNPLHFVVTHEAHYQLREKGDRAVLVERPEGM
                     TPHAFANRAPATNERIMKLLTGFIPGGHMKTERHFHPQIYPVTGEIQRSEDGRANRIP
                     TSKPDMRSSFAEDIYIQLGSIQDARRKTNPDFAHIYEFYYFEFQKSPEAYQQLFPPVI
                     VATLEVWVNPLVKLIWLGSVMFFLSGLILVLPYGERK"
     gene            complement(3716..4192)
                     /locus_tag="PLG78_10215"
     CDS             complement(3716..4192)
                     /locus_tag="PLG78_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNE23534.1"
                     /translation="MKTRFWVLMILIIGSLAAMVIFTGGSKNTSVMLADASHVVDQPS
                     KYQEHELRVRGFVKPGTILRYGDKADFVMTLDGRDLKVHFDGGTQLPDTFTDAAPVRA
                     DGRLAPDTAGLLVANKVEAKCASKYEADYAKKGDFTSARFQRMAADEKVPGGDHPK"
     gene            4207..>5797
                     /locus_tag="PLG78_10220"
     CDS             4207..>5797
                     /locus_tag="PLG78_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE23535.1"
                     /translation="MAHSVWQAISMNEFSRKEFLKKTLSWTGKSAAAGIVGGTAYNFF
                     GHSEYKQAFPNIPENHANVQSNGKTVLILGGGLAGIQAACELADRGFQVTVLEKSGHA
                     GGKLKSWKDKKFAQKYFPDGYSREHGLHGVWGFYKNLREFLGRHQIPLNPLGEKESFY
                     SFIDAQGVRNKIKPVKWPIPFDRIEMMNNGIYVPDEKDLKKPAPDLFNAFRATMKLSG
                     FNFKSESQRMYLDSMTFYDWAKKLGVHDQFIRYYYEAMSDMALFMSTKECSALGIANL
                     IRLGATPSETRVDYYKWPPDETVIGPLVKHLEMKGGRVLFHNEVTELVVENGKVTAVK
                     TNQNLPAGSVRRCRICGNLIYGGVEDHCPYCGADASHIEVMTEQEKVPKTFRADHYIL
                     AMDIPGAKHVLMNTDLARQYEYFRNIQNLSQSTILCVNLLYENSDAWERMFPSGTTLS
                     AVDFFPTGFKILGFTSNWSSTGIPGLRQKKVDLIEVQVCRWQQFIGKDFRSIAAAVHA
                     ELKTALPDLPEPTDFYINRWDT"
BASE COUNT         1600 a         1510 c         1436 g         1251 t
ORIGIN      
        1 agtctgccgt aaatcgcacg cgaagagccg gacccagatc gggaatttct acctggtctg
       61 cttccccacc ttgaaagaca ggcttcgcat ctgcaggctg atgaagaagc acgatgaatg
      121 gatgaaccgc gggacttcct gcgagttgat gaggacgttc ctccagaaca gttccctcta
      181 cgtcgtactc aatggtgagt tctgcgcttc ccggacgaag agaacgcgaa agaacaccgt
      241 ctacaccgag cacattgggc aatggcatag aaccttgatg ttgcagctga ttctggacat
      301 ctgtggcgcc tgacggtata aaaattttaa gatcgtttcc ccgataactt ctcggcggat
      361 tcgagcgatt ctcaagagca tacacgcgcg aaatgtgcaa agtctccttc ttcttgatta
      421 cctgcatgcc ggacgtcacc ataatgtctg acggcttcgc gccagtctca tagaccataa
      481 ccttctgaaa agaaccggtt gctcttgccg tgggaggaat cacctggatg tagttttcac
      541 cctgaaacgt agcacgcaca agaagagggc cgtttcctgc ttccacagga gcaaacttaa
      601 aaacactgga agatggcttg atggtttcta gaacctgcat gccctgaccg ggccgcaata
      661 gagcaatttc atctgcagca accgcaacgc cagtcgttcc attggtcaca gatccttgaa
      721 tcacaaccgg acctcgagcc atagggcctt gctgtccggg catctgcatt ggctgagatt
      781 gaatggcgcc taacggcaac agaatcattc cgaaggcaag caagacgccc agagtagtct
      841 ggcgaatcat tgcttcacct ctgtgccaca ctgcggacac atctcaaatg gcgctcccag
      901 atacccgcac acagcgcaac tttgtctatt tgatgaaatc gccttcttgc gcgaaggtgc
      961 tggaatgctg gccagcgaat ccaacctgtt tgcgagagga gttgccatct gggaaaactc
     1021 ttcttctgag agtttgcccg catcaaaatc ctgcctcaat tccgcaagta cactctggag
     1081 ttgcgcgcgg gtttccgcga ttctctcatc ctgaggagct cctacgtagt actccggtgc
     1141 cgatcttgac cggagaagag ggaagaagag ggcgaggatg caaccaacca gagcgatcgt
     1201 gagcagaatg ctcatttgcg ttccccgtac ggcagaacca gaatcaaacc agaaaggaaa
     1261 aacattacag aaccgagcca gatcaatttc acgagcggat tcacccacac ttctagagtg
     1321 gccacaatca caggcgggaa gagttgctgg tatgcctctg gacttttctg aaattcaaag
     1381 tagtaaaatt catagatgtg cgcaaagtcc ggattggttt tgcgtctcgc gtcctgaatg
     1441 gatccaagct ggatgtagat gtcttctgcg aaagaagaac gcatgtctgg cttactggtt
     1501 ggaatgcgat tcgctcttcc atcttcactg cgctgaattt ctccagtcac gggataaatc
     1561 tgcggatgaa aatgcctttc tgtcttcata tgcccgcccg gaataaaacc ggtaaggagc
     1621 ttcatgatcc tttcattcgt ggcaggcgcg cgatttgcga aggcgtgagg agtcatccct
     1681 tctggcctct ccacaagaac ggctctgtcc cctttttcgc gcaactggta gtgagcctca
     1741 tgagtcacga caaaatgaag aggattgttc tggtccgcat tggccagaaa gtatggggca
     1801 aaaaacaaat ctccagcttc cacttcgtaa ttgtctacgt atgatttatc tccgctgtaa
     1861 tagtgcactt ccgaactgcc tgggtactgc ggcattctat aatacatgaa atccattttg
     1921 actgttttct taaacgatcc gcccgcatat cccaggaaca aaaacaccac agaaagatgc
     1981 accagataac cgccgtatct tcgcttgttt cgtccgatca aatgcaagaa cgcctgaacg
     2041 gagttttcac ccagcctgac acgcctggaa cgtatcccgc gagaatattc ctgcacaatg
     2101 ccggctatcg taaaaactgc aattccaacg gtaagaatag aaaagacttc cgccacgaga
     2161 gacgatcccc aggcgctatc ctcaccggac ggcttgaaca tttttccgta taacagagca
     2221 aaggcgattg taaccaccac accaaggatc aacgggatcc tgaggttctt cacgtacacc
     2281 tgatccgcgc ttttcttcca cgcaaagagc ggagaggccc ccatcaagaa cagagtgagc
     2341 acacccacag gaatcatgat gcgattgtag gcggtcagct tccactctgc tttgctgcag
     2401 gagaaaccgt catcgaatct gcaatccagc ggaatgagcg gagaaaaaac cccgattaga
     2461 atcacaaaca cactcaacaa catcaggaag ttgttgagaa gcatgcttcc ttctttagac
     2521 gtgatggtct gcagctcctc atctggtcga agcctcttcc tttccatgat gatatatcgc
     2581 aaatagaagt agaaactcac tccaatgtaa atgatgaggg gtttgccaat gctcgactca
     2641 gaaaagctat gcgggccctg gagcactccg cttcgagtga tccacgtgcc cagcatgcta
     2701 aagtggtacg cgagaagaat caaaagcatg gtccagaatt tcaaaagacc gcgctgttct
     2761 gatacgatcg cagtgtgaag aaatgctgtc atgagaagga aaggcatgag gctcgcgttc
     2821 tccactggat cccaggccca gtagccaccc catcccagtt cttcgtaggc ccacttcgaa
     2881 cccagcagaa tccccgtgcc cagaaaaaac caggagaaga tcgtccatct gcgaagaagt
     2941 ggatagaacg cattacgagt gttacgcgaa acaagaacag acatggcgat cgcgaaagga
     3001 atcgcggagc ttacgtaccc aacatataga atgggaggat gaatgatcat gccccagtgc
     3061 tgcagaagag gattgagacc ctgacccgct ttcattggct gcgggaaaac tctaaagggt
     3121 tgcgcgtcgc tgaagtagag tcccatgaac acgaagatga actgcaatcc agcaagaacc
     3181 agaaagagaa acggcatgcg attgcgcaga attctgctct gataaaccgc aacggcgctg
     3241 agagaagtca gaatgaaata ccagaacaaa aggcttccgc tcgaaccggc ccaggcgcca
     3301 gtgatgaggt agaagatgtg cagatccgca gaggaatgtg taactacata gtgattggtg
     3361 aggtccccct gcaccatcag caccatcagg gtgaacatgg caaaggatgc agccgcagcg
     3421 ttcacgtaga gagcagatcg agcaagatcc agcgcaaccg aggcacttct aaaaccaaga
     3481 agagcaatca gcctgcgacg cactaccatc cagacagaca caacggcgtg agacatgcga
     3541 tgcgacagcc tgcgccttcc gcgcaacttg cgaaagtcgt ctgcaagctc aaaaggtaga
     3601 aatcgtatga gagaacgaat ttgccggatg ctcatgccga gcaaagacat gaaaatagaa
     3661 aacgttacta gagcggcaga gagaagtagg aacgccgcac cgatttgatt cagattcact
     3721 tcgggtgatc tccgcccggc actttttcgt ccgcggccat gcgctgaaag cgagcactcg
     3781 taaaatcgcc cttcttcgcg tagtctgctt catacttgga tgcgcatttc gcttccacct
     3841 tgtttgccac aagtaggccc gccgtatctg gcgcaagacg gccgtctgca cgaaccggag
     3901 ctgcatctgt gaaagtgtct gggagttgag tcccgccatc gaagtgcacc ttcaaatcgc
     3961 gaccgtctag agtcatgacg aaatcagctt tgtctccata gcgaaggatc gtacccggct
     4021 tcacaaatcc gcgcactctc agttcatgtt cctgatactt cgatggctga tctacgacgt
     4081 ggcttgcatc tgcgagcatt acggatgtat tcttgcttcc gccggtaaag atgaccattg
     4141 cggcaagtga gccgatgatg agaatcatca gaacccagaa tcgggttttc ataattgcaa
     4201 gaaaaaatgg cgcacagcgt atggcaagca atcagtatga acgagttttc gcgaaaagaa
     4261 ttcctcaaaa aaacactgtc ctggaccggc aagagcgccg cggccggaat tgtaggtgga
     4321 acagcctaca acttcttcgg acattcggaa tacaagcagg catttcccaa catacctgag
     4381 aatcatgcga atgttcagtc aaacggaaag acagtcctca ttctgggtgg aggactcgcg
     4441 gggatccagg ctgcctgtga actcgccgat cgcggcttcc aggtaactgt acttgaaaaa
     4501 agcggtcatg caggcggaaa actcaagtcc tggaaagaca agaagttcgc tcagaaatac
     4561 ttcccggacg gctactcgcg cgaacacggg ctgcacggag tctggggctt ctataaaaat
     4621 cttcgcgaat tcctgggaag gcatcagatc ccactcaacc ctctgggaga aaaggaatcc
     4681 ttctatagct tcatagacgc gcaaggagtg cgcaacaaaa tcaagcctgt caagtggccc
     4741 attccgttcg atcgaatcga aatgatgaac aacggaattt acgtgccgga tgaaaaggat
     4801 ctaaaaaagc ctgccccaga tctattcaac gcatttcgcg ccacgatgaa actctcaggc
     4861 ttcaacttca aaagcgaaag ccagagaatg tacctcgaca gtatgacctt ctacgactgg
     4921 gccaagaaac tgggcgtaca cgatcagttc attcgctact actacgaagc aatgtcggat
     4981 atggcgcttt tcatgagcac gaaagaatgc tccgctctgg gaattgccaa cctcattcgc
     5041 ctgggcgcta caccgagcga aaccagagtg gactattaca aatggccgcc agatgaaacg
     5101 gtcatagggc ctctggtaaa acatctagaa atgaagggcg gcagggtgct ctttcacaat
     5161 gaagtcacag agcttgtagt agaaaacggc aaggtaacag ccgtgaagac aaaccagaac
     5221 ctgccggccg gaagcgttcg tagatgtaga atttgtggca acttgatcta cggcggcgtg
     5281 gaagatcatt gtccttactg cggcgcagat gcatctcaca ttgaagtgat gacagaacaa
     5341 gagaaagtgc ccaagacttt ccgcgcagac cactacatcc tggctatgga cattccaggt
     5401 gccaagcacg tgctcatgaa cacggatctg gccagacagt acgagtattt tagaaacatc
     5461 cagaatctga gccagtctac gattctgtgc gtaaatcttc tatatgagaa ctctgacgcc
     5521 tgggagagaa tgttcccgtc tggcacaact ctgagtgctg tggatttctt ccctaccgga
     5581 tttaagatcc ttggattcac gagcaactgg tcgagcactg gcattccagg cctcaggcaa
     5641 aagaaagtgg atctaatcga ggtccaggtt tgtcgctggc agcaattcat tggaaaagac
     5701 tttcgatcca tcgcggctgc cgtacatgca gagctcaaaa cggccctgcc cgatctgccc
     5761 gaaccaacag acttctatat taaccgctgg gacacct
//
LOCUS       DAOQJL010000208         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7927_length_5792_cov_3.657661, whole genome shotgun sequence.
ACCESSION   DAOQJL010000208 DAOQJL010000000
VERSION     DAOQJL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_5792_cov_3.657661"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PLG78_10225"
     CDS             complement(<1..1198)
                     /locus_tag="PLG78_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23536.1"
                     /translation="MPVVLFLVASAFLGSIAFAIFLRRLDTPGLKINQVRRQGELQTE
                     KLDEIARNQIQAIRDATIEFELIVRQSRKLKDELRNDLEQYQNRIQSLRSDRDVLDTV
                     SVDLAQVAGSARTVASQVDRLDSGLQRLAYAQEEIAGIRKQLDDLSSISERKGQEAEI
                     RLGEIISRLVQDTETSTRLLTDQTRSNVSNVKDEFQDLTYKLEDQFKQVQVVSERISS
                     LNMRLDEKWSQDASRLDEKFAQQEKNYLDRISSIEAGLGAIRQTAVEALQSEVARIRR
                     DLDGFNLEGISRRDEILSESRRMAEMMNTQIADFNEKHLAAESRLQRISEQQKNVLKE
                     KLEALTKEWDALEETRLASIETRIRGIEEEFDRLRAEQAEHLATEAHQAIEQIRAESG
                     EATALLR"
     gene            1307..2056
                     /locus_tag="PLG78_10230"
     CDS             1307..2056
                     /locus_tag="PLG78_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE23537.1"
                     /translation="MQIVGGSVRTVPIYSAFAMIYDEVMSHVPFQNWARYLAAQAGRR
                     QRLLDLACGTGKVLQTLLQYTAADTIGIDSSEPMLSIARRRLKGQAEIRPGNLTRIPV
                     EDASADWAICTHDSINYLMTEGQLTRHFKEVFRVLEPGGIYSFDAVTEQNMITEFDGQ
                     VREEMIDGVLLRWSNRYERDRRVLVSHLVFQDGSSIQVEMHEQQYYDSETILSLASRA
                     GFETGPVQGDYEDRPLRARDAFMNFNCTKPL"
     gene            2074..2466
                     /locus_tag="PLG78_10235"
     CDS             2074..2466
                     /locus_tag="PLG78_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNE23538.1"
                     /translation="MKFLNYRDRSTEEVRRYLRRKNIVSEAEIPAVIKKLEDLGFAND
                     IRFTENRIRYRIGNLYGPFYIQRELAGLGISAETFRPLLAELEPEFVDAARQYAAKIK
                     GGLDGPRLEKRLLGRGFSTNQVKLALKA"
     gene            complement(2413..2856)
                     /locus_tag="PLG78_10240"
     CDS             complement(2413..2856)
                     /locus_tag="PLG78_10240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002732758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE23539.1"
                     /translation="MTPGFFQISLKLFLMRGDELLILRDRASKYGDLPGGRLGETEIY
                     LPFPESLGREIREELGESVRYSLNPEPLFVFPHKMIAGHEALGIAFIGRYEGGEIELS
                     EEHDQMKWENVRSFDPRSVFFAHLLDAVLRFQSEFDLIRRKAPAE"
     gene            complement(2871..4049)
                     /locus_tag="PLG78_10245"
     CDS             complement(2871..4049)
                     /locus_tag="PLG78_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23540.1"
                     /translation="MNYGFLSNYNRYREEGSREAGEQLAGHLHRLLSAWTETGSPDQD
                     FLEQFQEFMEELQTLPPDQNDLLDLGLMHAVEAFNLLKENRPEEALRIASDARFPFLA
                     YLDENSYRSLTNVTFHEIDFQIYERIRGNFPEESARNYLLNAKRTDLWAAIRYLEGLE
                     NREEAIEILSGMIERRIVLPERLILLAFWLLLDPDILTSERRPFFEQEQLLESLARVA
                     SPGMATEGLDTGWVERMNPLYENEILFFYQAYFEISGSPLSPGWIHLLEKSISNHWRV
                     TIPEFPDEVHEPNPEFAGSILHLIEDEYIENLLKTSLILPLFFENLEKYVDFSFYEIS
                     SGIARKEAIFMEELGHFLGRLTSEISELLRNRLEECAEILDCDLTHSDRGFILQRRHI
                     "
     gene            complement(4046..>5792)
                     /locus_tag="PLG78_10250"
     CDS             complement(4046..>5792)
                     /locus_tag="PLG78_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNE23541.1"
                     /translation="SILNISLYASTFNYSVNEKHLGWEFFAYINDLGSLWRDVFEKDV
                     ASGLFYGLVPLVFLAGAIAVFRKQGGFHSDEPRRFETRFKKRKLFATLGIFVLLGIFL
                     RGGLQQSPLRTADAMTTDSPYLNNLKLNGIFTVLNDSRDSLEFKSFYSREENIKTMTQ
                     FLSAGKISKDYPLLRRMPPRTLASARPVSHPRVILVVLESFSSKFLAVHGYDPSIAPQ
                     FNELIHRGVYFRRFFATGGRSANGLFAMFAGIPDRAGRTILRSSQIQNRFGGLAGILG
                     RKGYRTFFVHGGDLKFDNLNTALPHLGFSESYGRDEIAATGFQAEPNVLGYDDRDAFR
                     FFLGQMDKKDQSFGVIFTQNTHFPYIAPDYKDYSKDRVANLFLSSYHYVDSLIGEFVR
                     ELEKRPYAKDTIVLFVADHTHHAGLNYIEDRSIPFLVYAPSLFSPAQIDFDASQLDIL
                     PGIVSLTGGNALYASMGNDPFLRAGLVRGEPEVLPEPFAFFAGGSNTNVIGWIQKDRI
                     AIRWLDRDTPLFLTSTPPITGENFALKEKSRLDDYTAHMRHYHQYARTLEKENRIWPE
                     PDELAKLEKDGKASP"
BASE COUNT         1425 a         1423 c         1465 g         1479 t
ORIGIN      
        1 cgcgaagaag cgcagtcgct tctccagact cagcacgaat ctgctcaatt gcttgatgag
       61 cttccgtggc caggtgttct gcctgttctg cgcgcaggcg atcgaactct tcttcgattc
      121 cacgaattct ggtttctatg gatgccagtc ttgtttcttc cagtgcgtcc cattctttcg
      181 taagcgcttc cagtttctct ttgagaacgt tcttttgctg ttctgaaatg cgttgcagtc
      241 tgctttctgc ggccagatgc ttctcattga agtccgcaat ttgcgtgttc atcatctctg
      301 ccatgcgacg gctttcactt agaatttcat ctctgcgcga gataccttct agattgaatc
      361 catcgaggtc acggcggatg cgcgccactt cgctttgaag agcttctacc gctgtttgcc
      421 ggatcgcgcc aagtcctgct tcaatagaag aaattcgatc gaggtagttc ttttcttgct
      481 gagcaaattt ctcatcgagg cgcgaagcat cctgagacca tttttcgtcc aggcgcatgt
      541 tgagacttga gattctctca gatactacct gcacctgctt gaattgatct tccagtttgt
      601 aagtgagatc ctggaattca tctttcacat tggatacatt ggatctggtc tggtctgtaa
      661 gaagtcgagt ggatgtttct gtatcctgca ccagacgcga aatgatttcg cccaggcgaa
      721 tctccgcttc ctgtcccttg cgttctgata tagaggaaag atcgtcgagc tgcttgcgaa
      781 ttcccgcgat ttcttcctga gcataggcga gtctctgcag gccggagtcc agtctgtcca
      841 cctgcgaagc aactgtgcga gcgctgcccg ccacctgcgc caggtccacg gacacagtgt
      901 ccagaacgtc gcgatctgag cgcagactct gaatgcgatt ctggtattgc tccaggtcgt
      961 ttctgagttc atctttcagt ttacgggatt gacgcacgat gagttcaaat tctatcgtcg
     1021 catcacggat cgcctggatc tgatttctgg caatttcgtc cagcttttcg gtctgcagtt
     1081 caccctgacg gcgaacttga ttgatcttca ggccaggtgt atcgagccgt ctgagaaaga
     1141 tggcaaatgc aattgagccc agaaaagcgc ttgcaaccag aaatagtaca acaggcattt
     1201 ccaccataat tcagccccga aagcgtaatg cgacccgcat gatattatgt cgcatatgcg
     1261 agcagggcag gaatccacgc acatggcaag aaaaaaagga gaatccgtgc aaatagttgg
     1321 aggttctgta agaaccgttc ccatctattc tgcgtttgcc atgatctacg atgaggtcat
     1381 gtctcacgtt cctttccaaa actgggccag atacctggcc gctcaggccg ggaggcggca
     1441 gcggttgctc gatctggcat gcggcacagg caaagtgctg caaactttgc ttcagtacac
     1501 tgccgccgat acaatcggga ttgattcctc cgagcccatg ctttccattg cgcggcgtcg
     1561 tctcaaaggg caggcggaga ttcggcctgg aaacctcacc agaatcccgg tagaagatgc
     1621 cagcgcagac tgggccattt gcactcacga cagcatcaac tatctaatga cagaaggcca
     1681 gctcaccagg cactttaaag aggtctttcg cgtcctcgaa ccaggcggaa tctattcttt
     1741 tgacgcagtt acagagcaaa acatgattac ggagttcgac ggccaggtcc gagaggaaat
     1801 gatagacggt gtacttttgc gctggtccaa tcggtatgag cgtgaccggc gagtgcttgt
     1861 ttcgcatctt gtctttcagg atggctcgag catccaggtg gagatgcacg agcagcaata
     1921 ctatgattcc gaaactattt tgtctcttgc gtccagggca ggatttgaaa caggcccagt
     1981 ccagggagac tacgaagaca gaccactgcg cgcacgcgac gctttcatga acttcaactg
     2041 cacaaagccc ttatgaatat tccagaacca gtcatgaagt ttctgaacta cagggacaga
     2101 tctacagaag aagtgcgcag ataccttcgc cgcaagaata tcgtaagcga agcggaaatt
     2161 ccggccgtga tcaagaagct cgaagatctg ggattcgcta acgacatacg cttcacagag
     2221 aacaggattc gataccgcat tggaaatctg tacggccctt tctatattca gagggaactg
     2281 gcaggtcttg gaatttcggc agagacgttt cgtccgcttc ttgcggaact tgagccggag
     2341 tttgtggatg ctgcgcgtca gtacgcagcg aaaatcaagg ggggcctgga cgggccaaga
     2401 ctcgaaaagc ggctactcgg ccggggcttt tctacgaatc aggtcaaact cgctttgaaa
     2461 gcgtaagacc gcatccagaa gatgtgcaaa gaaaacggaa cgaggatcaa aggatctcac
     2521 attctcccat ttcatctggt catgctcttc tgatagttcg atttctcctc cttcatacct
     2581 gccaatgaac gcgatcccga gtgcttcgtg gcctgcgatc atcttgtgcg gaaaaacgaa
     2641 tagtggttct ggattgagcg aatatcgaac gctctctccc agctcttctc tgatttcgcg
     2701 acccagggat tcaggaaaag gtaagtagat ttcagtttct ccgagcctgc ctcctggcag
     2761 gtcgccgtac ttacttgctc gatctcgcag gatcaagagc tcgtcgccgc gcatcagaaa
     2821 tagttttaag ctgatttgga aaaatccagg agtcatacgt tattccgttc tcaaatgtgg
     2881 cgccgctgca aaatgaaacc tctgtcagaa tgagtcagat cgcagtccag gatctcagcg
     2941 cactcctcca gtctatttcg cagtagttct gatatttcag acgtcaggcg accaaggaaa
     3001 tgcccgagtt cttccatgaa gatggcttct ttcctggcga tcccggatga gatttcataa
     3061 aaggaaaaat ctacatactt ctccaggttc tcgaagaaaa gaggcaaaat caggctggtt
     3121 ttcagaaggt tttctatgta ttcatcttca atcaggtgca ggatgctgcc cgcaaattct
     3181 ggatttggct cgtgcacttc atctggaaac tctggaatgg tgaccctcca atgattcgaa
     3241 atgcttttct ccagaaggtg aatccatcca gggctcagcg ggctgccgct gatctcaaaa
     3301 tacgcctgat aaaagaatag aatctcattt tcatagagtg gattcattcg ttctacccag
     3361 cctgtatcga ggccttctgt tgccattcca ggagaagcaa cacgcgccag ggattcaagc
     3421 agttgctcct gttcaaaaaa tggcctgcgt tctgaagtca ggatgtctgg atcgagtaac
     3481 agccagaagg cgagaaggat cagccgttcc ggtagaacga tgcgtctttc tatcatcccg
     3541 gagagtattt caattgcttc ttctctgttc tccaatcctt ccaggtatcg aatggcagcc
     3601 cacagatctg ttcgtttggc gttcagcaaa tagttgcgcg cggattcttc cggaaagttt
     3661 ccgcgaattc tctcgtagat ttgaaaatca atttcgtgaa aggttacgtt ggtgagactg
     3721 cgataggagt tttcatccag ataggcaaga aatggaaatc gagcatccga tgcaatgcgc
     3781 aacgcttctt ctggacgatt ctctttgagc agattgaatg cttccacagc atgcatcaaa
     3841 cctagatcga gcagatcgtt ttgatcggga ggaagagtct gcaactcttc catgaattcc
     3901 tgaaactgtt ccaggaaatc ctggtccggg ctgccagtct ctgtccaggc actcagaagt
     3961 ctgtgaaggt ggcccgcgag ttgttcgcct gcttcacggc tgccttcctc tcgatagcga
     4021 ttgtagtttg ataagaatcc gtagttcaag gggaagcctt cccatctttt tccagtttcg
     4081 caagttcgtc tggttccggc cagatccggt tctctttttc cagggtgcgc gcatactgat
     4141 gatagtgacg catgtgcgct gtgtaatcgt ccagcctcga tttttcttta agggcaaaat
     4201 tttcgccggt gattggagga gtggaagtca aaaacagcgg agtatcgcga tccagccaac
     4261 gaatggcgat tctatctttt tgaatccagc ctattacatt ggtgttggac cccccggcaa
     4321 aaaacgcgaa tggctccggc agaacttctg gttctccgcg aaccaggccg gcgcgaagga
     4381 atggatcgtt gcccatggag gcgtagagag catttcctcc ggtcagcgaa acgatccctg
     4441 gaagaatatc cagctggctt gcgtcaaagt cgatttgtgc aggggagaaa agagaaggag
     4501 catacaccag aaaaggaatt gagcgatcct ctatataatt caatcccgcg tgatgtgtgt
     4561 gatctgcgac gaagagaacg attgtgtctt tcgcatacgg acgcttctcc agttctctca
     4621 caaattctcc aattagagaa tccacgtagt gatagctgga caggaagaga ttggccactc
     4681 tatcttttga ataatctttg tagtccggcg caatgtatgg gaagtgagtg ttttgtgtga
     4741 agatgacacc aaaagattga tccttcttat ccatttgtcc aaggaagaac cgaaacgcgt
     4801 cgcgatcgtc gtatccaagt acattcggtt ctgcctgaaa tccggtggct gcgatttcgt
     4861 ctctgccgta ggattccgaa aagccaagat gtggaagggc agtgttcaga ttatcaaact
     4921 tcaggtctcc gccatgaaca aagaatgtgc gataaccttt tctaccgagg attcccgcca
     4981 gtccgccgaa gcggttttga atctgcgacg accttagaat cgtccggcct gcgcgatcgg
     5041 ggatcccggc aaacatagcg aagaggccgt tggcgctacg gccgcctgtc gcaaagaaac
     5101 gacggaaata cacaccgcgg tggatcaatt cattgaattg cggagcaatg gaaggatcat
     5161 atccatggac cgccaggaat ttggagctaa acgattcgag caccacaagg atgactctcg
     5221 ggtgcgatac tggcctcgca gaagcaaggg tgcgaggcgg cattcttctg aggagtggat
     5281 agtcctttga tatctttcca gcagaaagaa actgagtcat agttttgata ttctcttcgc
     5341 ggctatagaa cgatttaaat tcgagagaat cgcgcgaatc gtttaacact gtgaagatgc
     5401 cgttcagctt gagattgttg aggtagggcg aatcggtagt catcgcatct gcggtgcgaa
     5461 gagggctctg ttgaagaccg ccgcgaagga agatccctag caaaacaaag atccccagag
     5521 ttgcgaagag ctttcttttc ttgaatctgg tttcaaatcg gcgcggttca tccgagtgaa
     5581 agccaccctg cttgcgaaat actgcgatgg cccccgccag aaaaacgaga gggacaagac
     5641 cgtagaagag tccagacgct acatcttttt cgaatacatc acgccacaaa gatccgaggt
     5701 cgttgatgta ggcgaagaat tcccagccca gatgtttttc attcacactg taattgaaag
     5761 tggatgcgta cagactgata ttcagaatag aa
//
LOCUS       DAOQJL010000209         5782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_7954_length_5782_cov_5.194168, whole genome shotgun sequence.
ACCESSION   DAOQJL010000209 DAOQJL010000000
VERSION     DAOQJL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7954_length_5782_cov_5.194168"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2387
                     /locus_tag="PLG78_10255"
     CDS             111..2387
                     /locus_tag="PLG78_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23542.1"
                     /translation="MADIPGLSGPIDYHKAITKKERSSRYIRTAFDYLVQVQGEDALN
                     ELLREVGIERDSSIFKHIYDDENWNSYELEVFLYEKLKDRFEDPYQAIWQFGIASGSG
                     RLDQKDTLFAFKLKIAPVPVILRKVSEHTERVTLVSECNAEILKTPATHIKNGMAATI
                     SFKYSRLPENFQYPSWTSVVAGYAIVYATVRYRKGLDFYLKITHWPNLPSDLPSVDGK
                     VYVFDKVTKNVSEKETGKVVGNARNGPFEIDGVVWNNGVEAIAWIEWERESLGQAIAK
                     LTIHRKRLKKEEELRVLRNEIVEELSAQHQKQLAAYEKELTEKARLIQAHLEKIHELK
                     IQQDGDYFLTSLLTSPMIANRNKSDAVQTEIHVEQKKKFEFRKWKSELGGDTCLTDQV
                     ILRGRQYSVFLNGDAMGKSLQGAGGAIVLGVVFNTYLNRSRFSSYQKQKSPELWLRDA
                     ALDLQNAYLSFQGSMYVSLVMGLIDETSGVMYFINAEHPWMVLYRDGKASFIGDELLL
                     RKLGTPGMEDQIRINTFQLKPGDSIIMGSDGRDDLEIGHDGSNRVINEDETIFLKVVE
                     ESKGNVESIVKNIKKQGSITDDLSLLKVSFFGSPANIENANAEYKASLGKAVALGRLN
                     KLDEAIQILHSLYRAHPDRVEPLRYLGRLQFKQKDYPAAAQTLTNFFEQHQDESGVQY
                     LISRASKKAGDYKAAIDFGERCMLREPEHLNNLINLADAYRLSGNTDRARQLVTRAVS
                     LSPDHAAARKLANLMDRS"
     gene            complement(2384..2695)
                     /locus_tag="PLG78_10260"
     CDS             complement(2384..2695)
                     /locus_tag="PLG78_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23543.1"
                     /translation="MKRILLSFLIVLFGCTTAEDQTIDADEANTRIGIAIGLKNAQCG
                     YGQFLTLPAIFPLREEAVTLCVSAIISTPCSTWAGNNPLSGACLALPFYRKKSRDNYG
                     L"
     gene            2729..3637
                     /locus_tag="PLG78_10265"
     CDS             2729..3637
                     /locus_tag="PLG78_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE23544.1"
                     /translation="MTVDSSRIAGAVLVFLSAVAFSAKAVIIKLAFLGPVDPITLLTL
                     RMIFSFPFFLILAVISSKKSRSPGISRGDWFLILFCGVVGYYLASLFDFLGLQYISAG
                     LERLILFIYPTFVVLISVFAFGKRVTKQIAIALVLTYAGIAFVFWHDFGTFGENAWIG
                     GGLILLSAITYAFYLAIGGRLIGRLGSMRFTTLALLVSVGAVVIHFLLTRPLHLLSDV
                     DQKTYGYAFLMAIVCTVLPTLLLASGIRRIGSSAAAIIGSVGPVSTIALAWWFLDEHV
                     DVTHAIGTILVLSGVLTVSLQKSHEP"
     gene            3662..4249
                     /locus_tag="PLG78_10270"
     CDS             3662..4249
                     /locus_tag="PLG78_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23545.1"
                     /translation="MKSALILFSTTFLAVTGVSADEWNISTYQGLYSQTNLGQILLQG
                     KTHYQPGYISAATVGKPTDYGIFGLKLETEGQVVRHTGRDKHMEFNGLVMARTEELFN
                     LPITFAMAEGLSLATRNPDLENPRKSIGNPGTESEYSRNLLNYLAFEVEVGVPLDAYK
                     PRAFVRVHHRSGIYGILCPPTCGSNYLGYGMRFSY"
     gene            complement(4294..5151)
                     /locus_tag="PLG78_10275"
     CDS             complement(4294..5151)
                     /locus_tag="PLG78_10275"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE23546.1"
                     /translation="MVISIIGAGIMGKGIASNAARNGHHVRIYTRSRAKIEDILGPGI
                     EYFGKPEEAVTGAELTVVCLTEDHVVRAIVLSESFLSAISGSVMDVGTTSPELTDELF
                     RAFDKRGIRFFDAPMTGSKQAARDGKILFMMGGSDDDYAAGKPVFDSAGRAVVRCGKI
                     GDGQRAKIALNMVQAGTLQILIEGYHLSSLLGIEKTTFKEILDQSAARSGISDFKTPF
                     FLDRNYETNFSLKNMNKDLNHALSLAMSVRAELPSAFALKAVFDRAMNLGNAEKDFCS
                     IADATLNAD"
     gene            5231..>5782
                     /locus_tag="PLG78_10280"
     CDS             5231..>5782
                     /locus_tag="PLG78_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE23547.1"
                     /translation="MRLTIPLILLCAVCSRSGFKFADTFHPAFPAPNIALQGSKGPYD
                     FSKDRAENRQVVTLVFFGFTHCPDICPTTMHRLSQAMGALNPKEQARVRLLFITIDPQ
                     RDSPKIAGDYAAGFFPGFIGLSGSPGDIDKVKTAFSATSDSQGGEITHSSGIYWIDSE
                     GNVRKKISQNFEIEDMAHDLKMVS"
BASE COUNT         1535 a         1454 c         1366 g         1427 t
ORIGIN      
        1 attcctcaac aatatgtcta tccataggac attcctcttc gaatgggtga cagatcaagc
       61 gattcgcaat ttaggttgac ctggcagtca tcacctagca gcgttagctc atggcagaca
      121 ttccaggtct cagcggaccg atcgactatc acaaagcaat caccaagaag gaacgctctt
      181 ccagatacat tcgaactgcc ttcgattatc tggtgcaggt tcagggcgaa gatgctctca
      241 atgaattgct ccgtgaagtt ggaattgaaa gagacagttc cattttcaaa cacatctacg
      301 acgacgagaa ctggaattcc tatgaactgg aagtgtttct ctacgaaaag ctgaaagatc
      361 gtttcgaaga tccttaccag gctatctggc aattcgggat tgcatctgga tccggaagac
      421 tggatcaaaa agatacgtta ttcgccttta agctcaagat cgctccggtg cctgtgatcc
      481 ttcgcaaagt ttccgaacac acagaaagag tgactctggt ctcggaatgc aacgcagaga
      541 ttctcaaaac ccctgcaacg catattaaga acggaatggc cgccaccata tcttttaagt
      601 acagccgcct tcctgaaaat tttcaatatc cttcctggac ttcagtggtc gccggctatg
      661 ccattgtata cgcaaccgtg cgctaccgta aaggtctcga tttctactta aagatcactc
      721 actggcctaa ccttccgtcc gacttaccgt ccgtagatgg aaaggtttat gtgtttgaca
      781 aagtcacgaa gaacgtttct gaaaaagaaa ctggaaaagt cgtcggtaat gccaggaacg
      841 gaccttttga aatcgacgga gtagtctgga acaacggcgt ggaagccatc gcatggattg
      901 aatgggaaag agagtctctg ggtcaggcca ttgcaaaact aacgattcat cgcaagcgtc
      961 tgaagaaaga agaagaactg cgagtcttgc gtaacgagat tgtcgaagaa ttgagcgcac
     1021 agcaccagaa gcaactcgcg gcgtatgaaa aagaactcac agaaaaagcc agattgatcc
     1081 aggcccatct ggaaaagatc cacgaactca aaattcagca agatggagac tactttctta
     1141 cttctctgct aaccagcccc atgatcgcca atcgaaacaa gagtgatgcg gttcaaacag
     1201 agattcatgt agagcagaaa aagaaatttg aatttcgcaa atggaaatca gaactgggcg
     1261 gggatacttg ccttaccgat caggtaattc ttcgcggcag acaatactct gtcttcttga
     1321 atggagatgc gatgggaaag tccttgcagg gtgcaggtgg tgcaatcgtg ctcggagtcg
     1381 tattcaatac atacttgaat cgctccaggt tttccagtta ccagaaacag aaaagtccag
     1441 aattgtggct acgagacgca gctctcgatc tgcaaaacgc atatctatcg tttcagggaa
     1501 gcatgtatgt ttccctggtc atgggcttaa tcgatgagac ttctggtgta atgtacttca
     1561 tcaatgcaga gcatccgtgg atggttctgt atcgagacgg gaaggcgtct ttcatcggag
     1621 acgaacttct tctgcgaaaa ctgggaactc ccggaatgga agaccagatt cgcatcaaca
     1681 cattccagct caaaccaggg gattccatca tcatgggaag tgacggaaga gacgatctgg
     1741 aaatcggcca cgatggatcc aatcgcgtaa tcaacgaaga cgaaacaatc tttctcaaag
     1801 tagtagaaga atccaaaggc aacgtggaaa gcattgtcaa aaacataaag aagcagggaa
     1861 gcatcacgga cgacctttcg ctcctcaaag tttccttttt tggctcaccg gctaacatag
     1921 agaatgcgaa cgctgaatac aaggcgagtc tgggcaaagc ggtcgccctg ggccggctca
     1981 acaaactgga cgaggcaatc cagatcttac attctctgta tcgtgcgcat ccggatcgag
     2041 tagaaccact tcgctatctg ggaaggctgc agtttaaaca aaaggattat cccgcagccg
     2101 cacagactct taccaatttc ttcgagcagc atcaggacga aagcggcgtg cagtatttga
     2161 tttccagggc aagcaagaag gctggcgatt ataaagctgc catcgatttt ggagagcgtt
     2221 gcatgctaag agaaccagag catctcaaca atttgatcaa tctggcagac gcttacagac
     2281 tttctggcaa tacggataga gcgcgacaac tggtgacgcg ggccgtttca ctttcgccgg
     2341 atcatgcggc ggccagaaag cttgcaaatc tcatggaccg atcctagaga ccataattgt
     2401 ctcgcgactt cttcctgtaa aacggaagag ccaggcaggc gcccgaaagc ggattattcc
     2461 ccgcccaggt gctgcacggt gtggatatga tagcggaaac gcagagtgtg actgcttctt
     2521 ctctcaaagg aaaaatggcc ggcaaagtaa gaaactgtcc gtaaccgcac tgcgcattct
     2581 tgagtccaat ggcaattcca atgcgagtgt ttgcctcatc agcatcaatg gtttgatctt
     2641 cagcggttgt gcagccaaac aaaacaatca gaaatgacag aagaattctt ttcattttca
     2701 gggacgttcc tttcccagaa atggaaacat gacagtggat tccagcagaa tcgcaggcgc
     2761 cgtcctggtc tttctttccg ccgtcgcctt ctctgcgaaa gcagtgatca tcaagctggc
     2821 attcctcggt cctgtggacc cgatcactct actgactctt cgcatgatct tttcgtttcc
     2881 attcttcttg atcctggccg tgatatcgag caagaaatca agatcacctg gaattagcag
     2941 aggggactgg tttcttatcc tattttgtgg ggtcgtgggc tactacctgg caagtctgtt
     3001 tgattttctt ggacttcagt atatttcggc aggtcttgaa agactgattc ttttcattta
     3061 tccaacgttc gttgttttga tttcagtgtt cgcatttggc aaacgcgtca caaaacaaat
     3121 cgcaatcgcg cttgtgctaa cttacgcggg gatcgctttc gttttctggc acgactttgg
     3181 aacatttgga gagaatgcct ggattggcgg aggcctgatt ctactgagcg ccattaccta
     3241 cgccttctat ctagcaatcg gtggaagatt gattggacga ctgggttcta tgcgcttcac
     3301 cactctggcc cttctggtct ctgtgggggc ggtcgtgatt cactttcttc tcacgcggcc
     3361 gttgcatctt ctttcagatg tggaccagaa aacttacggt tacgcattcc tcatggcgat
     3421 cgtctgcaca gttcttccca cacttctcct cgcatctgga attagaagaa taggatcgag
     3481 cgctgccgcc atcattggaa gcgttgggcc agtatcgacc atagcacttg cctggtggtt
     3541 cctggatgag catgttgatg tcactcatgc aattggaacc attcttgtac tgtcaggagt
     3601 tctcacggtt agccttcaaa aatcgcatga accctgaggc aggtattccg tctaacggcg
     3661 gatgaaatct gcactgattc tattctccac gacattcctt gcggttacgg gagtttccgc
     3721 agatgaatgg aatatttcta cctaccaggg actctactcc cagaccaatc tcgggcagat
     3781 tcttcttcaa ggaaagacgc actaccagcc tggttacatc agcgctgcca cggtaggaaa
     3841 accaacggac tacggaatct ttggactgaa actggaaaca gagggccagg tcgtacggca
     3901 cacagggcgt gataagcaca tggaattcaa cggccttgtg atggctcgca cggaagaact
     3961 cttcaacctt cctattacat ttgccatggc agaagggctt tctctggcca ctcgcaatcc
     4021 cgatctggaa aaccctcgca aatccattgg aaaccctgga acagaatcag agtattcacg
     4081 aaacctcctg aactatcttg cctttgaagt agaggtgggg gtgccgctcg acgcatacaa
     4141 acctcgcgca tttgttcgag tgcaccacag gtctggaatc tacggaatcc tgtgcccgcc
     4201 tacgtgcgga agtaactacc tgggatacgg aatgaggttt tcctattgaa gatcgctctc
     4261 cttctcaacc ttctataaac tcgctttgca ccgttagtcg gcattgagag tggcatccgc
     4321 aatggagcag aaatccttct ctgcattgcc caggttcatg gcccgatcaa agacggcttt
     4381 cagtgcgaag gcagaaggga gttcagcgcg cacactcatc gcaagagaca gagcgtgatt
     4441 caaatccttg ttcatattct tcaaagaaaa attcgtttca tagttcctat ccagaaagaa
     4501 aggtgttttg aaatctgaaa tacctgaacg agccgcagac tgatccagaa tttctttgaa
     4561 ggttgtcttc tcgattccaa gtagagacga aaggtggtat ccctcgatca atatctgaag
     4621 agtcccagcc tgaaccatgt tgagcgcgat cttcgctcgc tggccgtctc caatcttccc
     4681 gcatctaacc actgctctgc ctgcagagtc aaagaccggt ttacccgctg cgtaatcgtc
     4741 atcgcttccg cccatcatga acaaaatctt tccatcgcgc gctgcctgct tgctccctgt
     4801 catgggagca tcgaaaaagc gaattcctct tttgtcgaaa gcacggaaca attcatccgt
     4861 gagttctggc gaagtggttc ctacatccat cacggaacct gaaatcgcag acaggaagga
     4921 ttccgaaagt acgatcgctc gcaccacgtg atcttccgtg agacaaacga ctgtcaattc
     4981 cgcaccggtg actgcttctt ctggcttccc aaaatattca attcccggcc ccagaatgtc
     5041 ttcgatcttc gcacgactcc tggtatagat gcgaacatga tgcccgttcc tggccgcatt
     5101 ggatgcgatc cctttgccca taattccagc gccaatgata gaaataacca tagttcccta
     5161 atcgatggat gcggactttc gtcgaacgca atttcattgc cggggacgac gacacaccaa
     5221 atcgttgcac atgcgcctta ccatcccgct aattcttctg tgtgcggttt gctcacgatc
     5281 cggattcaaa tttgcagata cctttcaccc tgcgtttccg gcgccaaaca ttgctttgca
     5341 gggatcaaaa ggtccgtatg acttctccaa agacagagca gaaaaccgcc aggtcgtgac
     5401 cctggttttc tttggattca ctcattgccc ggatatctgc cccaccacaa tgcacaggct
     5461 gtcgcaggcc atgggtgccc tgaatccaaa agaacaggcc cgcgtaagac ttttgttcat
     5521 caccattgac ccgcagcgcg atagtcccaa aattgcgggg gactacgctg ccggattctt
     5581 tccgggcttc attggattgt ccggcagtcc aggcgacata gacaaagtga agacagcgtt
     5641 tagcgccaca agcgattcac agggagggga aatcacacat tcttcaggaa tctactggat
     5701 cgactcagaa ggcaatgtgc gcaaaaagat ttcacaaaac ttcgaaatcg aagacatggc
     5761 acacgacctt aaaatggttt ct
//
LOCUS       DAOQJL010000210         5738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8038_length_5738_cov_4.918705, whole genome shotgun sequence.
ACCESSION   DAOQJL010000210 DAOQJL010000000
VERSION     DAOQJL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8038_length_5738_cov_4.918705"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLG78_10285"
     CDS             <1..207
                     /locus_tag="PLG78_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23548.1"
                     /translation="GRAFFSLRAEHSVIHVHRLVPIYTPITQLSLLQSGISSSLLPDL
                     IFKYGKTSGIDDYKSYEVGYEVML"
     gene            260..1300
                     /locus_tag="PLG78_10290"
     CDS             260..1300
                     /locus_tag="PLG78_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNE23549.1"
                     /translation="MSDPILAVVPLGFPWQTLDPFLFCVHHKDAYPQGDDRFAPRASL
                     SGRQIGNDFSYKDGWSMYHGEIVPGFPQHPHRGFETITVARAGFIDHSDSLGAAARFG
                     KGDVQWMTAGAGVVHSEMFPLLNKDSDNPLELFQIWLNLPAKSKMAAPYFSMYWDHLV
                     PKLELKDSAGHSIRLAAYAGPLDGKAITPPPDSWAAAAEADVRILTIRLDAGAQFEIE
                     KAGPGVNRVLYFFDGKSVSISGTVVKEKHAIQLNAEMNATIVATDGPCELLMLQGRPI
                     GEPVAQYGPFVMNTQEEIQNAFADYRRTQFGGWPWPDDAPVHPRDAGKFARHADGRVE
                     KPLDAMAASADL"
     gene            1346..1504
                     /locus_tag="PLG78_10295"
     CDS             1346..1504
                     /locus_tag="PLG78_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23550.1"
                     /translation="MNGKTAKLLRKFGAKKGSNVKDLKRVWMSLPAKERFKKRQEMLA
                     ELSGTKKS"
     gene            1591..2391
                     /locus_tag="PLG78_10300"
     CDS             1591..2391
                     /locus_tag="PLG78_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23551.1"
                     /translation="MNDSNAVTRIASRYSGPPRTGNGGYVCGVCAGLAYSQLGEQKQD
                     SFTFEVRYKSPVPVDLDVSLLSKDNLCVLESIDGKTTYAEGQSSKPVSTVLPAVVTLE
                     QARYATLQSIPEKEHAFPDCYVCGPARKVGDGLRILPGPVAEHSGMAAAVWNVLPDAM
                     SSSGDLSVPILWAALDCPGYFAYHFSHGGAMQVAVLAKMKCTVIRRRTESDQLVVIGW
                     MKRKKGPLVEVSTALLDGSEILAIADGTWMQPASWNPENTGHKSPQYS"
     gene            complement(2379..>3196)
                     /gene="mltG"
                     /locus_tag="PLG78_10305"
     CDS             complement(2379..>3196)
                     /gene="mltG"
                     /locus_tag="PLG78_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE23552.1"
                     /translation="LCLISVPVFANAGAHGGRKRPPEIIWKVRAGEKIRDLCQRLERE
                     GACLCTHFESTAKSADLSFIPRSEKTLRKFEGIFVPGEYRIEKIRGQDSQTRSARILG
                     SLLAAARTRYDHSDLEGNALHHRITIASIVEKESVKKENFEEVASVFFNRLRTGQTLG
                     SCPTVEYALGYHRPFLLESDVRIQSPYNVYRNKGLPPTPIAFFSEGAWNAALHPKETE
                     YQFFVFDWTTGKLGFSKSYEEHKDRASRARANFIRRFGAARLHAMDKTKYYEY"
     assembly_gap    3197..3296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3297..4060)
                     /locus_tag="PLG78_10310"
     CDS             complement(<3297..4060)
                     /locus_tag="PLG78_10310"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE23553.1"
                     /translation="MQKSLSAVRAVIPPHCMRPHAGRALLYMASGIIFYSASVAFAWF
                     VWEKSIYWLYPISWFVGGTASTSLFVLAHDCGHYSILKSRRIMDVLGHVLLLPVCYPF
                     YAWKHSHDAHHKYTNLLKREDSIYFDNAWIPETIEEYETEKSQRPFYAFLYRIARRFP
                     PLGAWMHHGHYLLSSPELYREDHRKKAVFSIWFVLIGMLTFGPAISFLSGSWFGAFHF
                     WFLPALCFQFWMAIYTFLHHTAEDVPFLPEGEWTPY"
     gene            complement(4060..5133)
                     /locus_tag="PLG78_10315"
     CDS             complement(4060..5133)
                     /locus_tag="PLG78_10315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE23554.1"
                     /translation="MQPPVVPSISQIRASIPNHCFQPSVWKSLFYFFFDISTIAGLAS
                     LALYLDSWYFYPVYWFLQGTMFWALFVVGHDCGHGSFSHIKWLNNLIGHLSHTPILVP
                     YHGWRISHRTHHQHTGDVDKDETWYPVTETQYSHMTLSIRILRYYVFLFLFPLYLFRR
                     SPGRDGSHFLPGSDLFRPSEKWDVLTSTLLWGIFFAGLCVVGWKFGIVFLAKFYIAPY
                     FVFVVWLDLVTFLHHTAPDVPWYRGEAWNFVRGNLSSVDRSYGIFEWFHHNIGTHMVH
                     HLFIGIPHYKLREATSHMAPFLGDLYRKSDESIFRAFWRAFRSCHIVSDEGPVVFYRP
                     AATLGQESDSGKPSASLKEAGAR"
     gene            complement(5133..5672)
                     /locus_tag="PLG78_10320"
     CDS             complement(5133..5672)
                     /locus_tag="PLG78_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23555.1"
                     /translation="MIESTASEAVRVTNMIKTFVQDGIPFTIELQKQGEGFYIGISEE
                     GKPDIKLPVETAQILNGDTFSIQTAAGETKGRFYFAGDEVYVHIAGQTFRMKSVSPAD
                     LETNTSNLHRSPMPGKVIAVQVAVGDSVQKDQTIVIVEAMKMENAIKATLSGKVKAVH
                     CKVGDLVSPDLNLVELEGS"
BASE COUNT         1434 a         1351 c         1486 g         1367 t
ORIGIN      
        1 ggccgtgcat tcttctcttt gcgcgcggaa cattctgtca ttcacgtgca caggcttgtg
       61 ccgatctaca ctccgatcac ccagctgagt cttttgcaaa gcggcattag ttcgtctctt
      121 ttgcctgatc tgatttttaa gtatggcaag acgtcgggca tagacgacta caagagttat
      181 gaggttggat acgaagtaat gctctagacg ttgctctttt tttgatcgcc cacgccagac
      241 acgctcttta tatagaagaa tgagcgatcc aatcctcgct gtagtcccac tcggtttccc
      301 gtggcaaaca ctggatccgt ttttattctg cgtgcatcac aaagatgctt atccgcaggg
      361 agatgatcga tttgctcctc gcgcttcctt gagtggcaga cagattggga atgatttttc
      421 ctacaaagac ggatggagca tgtatcacgg ggaaatcgtt ccaggatttc cacagcaccc
      481 gcacagaggt tttgaaacga tcacagtggc gcgcgcaggg ttcatagatc acagtgactc
      541 actgggtgct gccgccaggt tcgggaaagg agatgtgcag tggatgacgg caggtgccgg
      601 agtggttcat tccgaaatgt ttccacttct gaataaagat tcagacaatc cactggagct
      661 ttttcaaatc tggttgaatc ttcctgcgaa aagcaaaatg gccgctccct atttttccat
      721 gtactgggat catctggttc caaagcttga attaaaagat tcggcgggac acagcattcg
      781 cctcgccgct tatgctggtc cgctcgatgg aaaagcaatc accccaccac ctgattcctg
      841 ggctgcagcc gcagaagcgg acgttcggat tttgactatt cgcctcgacg cgggtgcgca
      901 attcgaaata gaaaaggccg gacctggagt gaatcgcgtg ctgtattttt ttgacggcaa
      961 aagcgtgagt atttcgggca cagttgtaaa agagaaacat gcgattcaac tgaatgcaga
     1021 gatgaacgcg acgattgtgg ccacagacgg gccgtgcgaa ttgctgatgc tccagggtag
     1081 accgatcggt gagcccgtgg cgcagtatgg accttttgtg atgaacactc aggaagaaat
     1141 tcaaaacgca ttcgccgatt acagaaggac tcagtttggc ggatggccct ggcctgacga
     1201 tgcgccggtt catccgagag atgcgggtaa gtttgcgcgt cacgcggacg gcagggtgga
     1261 aaagcctctc gatgctatgg ccgcctctgc ggacctgtga atcgctttta gttgacgcac
     1321 gtccccacct gcggacactt ggcccatgaa cgggaaaaca gcgaaacttc tccgcaaatt
     1381 cggtgcaaag aagggctcga atgtaaagga tttgaagcgc gtttggatgt cccttccagc
     1441 aaaagaacgc ttcaagaagc gtcaggaaat gctcgcagag ctttccggca caaagaaatc
     1501 ctgatcgctt tcatcatctc gaaattccgg cgcgaatttc aatatttttc aatgtgacat
     1561 aatccaggcc agggcccatt cggggggaac atgaacgatt cgaatgcggt gactagaatt
     1621 gcatccaggt attctgggcc tcctcgcaca ggcaatggag gttacgtttg cggcgtatgc
     1681 gcgggccttg cttactcaca gttgggtgaa caaaaacagg attcctttac gtttgaagta
     1741 cgctataagt ctcctgttcc agtcgatcta gatgtgtctc tgttatcaaa agacaacctg
     1801 tgtgtcctcg aatcgattga tggcaaaaca acctatgcgg aaggacaatc ttcgaagcct
     1861 gtttctactg ttttacctgc cgtggttaca ctggagcagg cccgttatgc gacattgcaa
     1921 tccattccag aaaaagagca tgcatttccg gattgttacg tctgcggccc ggcgcgaaaa
     1981 gtgggggacg gcttgaggat actcccggga cctgtggcag aacattcagg aatggcagcc
     2041 gcagtctgga acgttcttcc ggatgcaatg tcttcgagcg gagacctatc tgttccgatt
     2101 ctgtgggcgg ctcttgattg tccgggctac tttgcgtatc atttctctca cggcggcgcg
     2161 atgcaagtag ctgttcttgc taaaatgaaa tgcactgtga ttcgacggcg aacagagtca
     2221 gaccaactcg ttgtgattgg ctggatgaaa agaaagaaag ggccgttggt tgaagtgtcc
     2281 acagcgcttc tggatgggtc tgaaatcctg gcgattgcag atggtacgtg gatgcaaccc
     2341 gcatcgtgga atccagaaaa cacaggacac aaaagtcctc aatattcgta gtattttgtt
     2401 ttgtccatcg catgcaatct ggccgcgcca aatcgccgga tgaagttcgc tcgggctcga
     2461 ctggcgcgat ctttgtgttc ctcgtagctc tttgaaaagc caagtttacc tgtggtccag
     2521 tcaaagacga agaactgata ttctgtttct ttcggatgaa gggccgcgtt ccacgcgcct
     2581 tcagagaaga aggcgatcgg tgtaggcggg agtcctttgt tcctgtagac gttatacggc
     2641 gactggatgc gaacatcgct ctccagtaga aaggggcgat ggtaaccaag ggcgtactct
     2701 actgtagggc aggatccaag agtctggccc gtacgcagcc ggttgaagaa tacagaggcc
     2761 acttcttcga aattttcttt tttgacagat tctttttcca caatgcttgc gattgtgatt
     2821 ctgtgatgca gtgcatttcc ttcgaggtcg gagtgatcat atcttgttcg cgcggctgcc
     2881 aacagagaac ccagaatcct cgcagatcgc gtctgcgaat cctggccgcg aattttttcg
     2941 attctatatt ctccaggaac aaagatccct tcgaatttgc gaagagtctt ttcggatcga
     3001 ggaatgaatg aaaggtccgc gctttttgct gtgctttcaa aatgagtgca aaggcaggca
     3061 ccttctcgtt ctaacctttg gcagaggtct ctgatttttt ctccagcccg cactttccag
     3121 atgatctccg gcggacgctt gcgcccgcca tgtgctcccg catttgcgaa tacgggcacg
     3181 gaaatcaggc agagtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgt
     3301 aaggtgtcca ttctccctcc gggaggaaag gcacgtcctc tgcggtgtgg tgaaggaaag
     3361 tgtaaatggc catccagaat tgaaaacaaa gcgctggcaa aaaccagaaa tggaacgcgc
     3421 caaaccagga gcctgaaaga aaggaaattg ccgggccaaa tgtcagcatc ccgatcagaa
     3481 caaaccatat ggaaaaaacc gcctttttcc tgtggtcttc acgatagagc tcagggctgc
     3541 tcaatagata gtgaccgtga tgcatccagg ctcccagcgg cgggaatcgt ctggcgatgc
     3601 gatacaaaaa tgcatagaat ggtcgctggc tcttctctgt ttcgtactct tcgattgttt
     3661 ccggaatcca ggcgttgtca aagtagatgg agtcttctct cttcagcaga ttcgtatact
     3721 tgtgatgcgc atcgtgcgaa tgcttccacg catagaatgg ataacaaaca ggcaacagca
     3781 gaacgtggcc aagcacatcc atgatgcgcc tgctcttgag aatagaatag tggccgcagt
     3841 catgcgcgag cacaaacaat gaagtggagg cagttccgcc aacgaaccag ctgattggat
     3901 aaagccaata tatggatttc tcccatacaa accatgcgaa ggcgacggat gcagaataaa
     3961 agatgatgcc actggccatg tagagaaggg cgcggccagc gtgaggtctc atgcaatgag
     4021 gaggtatgac tgctcgaacg gcagacaatg acttttgcat tatcttgctc cggcctcttt
     4081 gagagaggcc gatggctttc cggagtcact ctcctgaccc agtgtggccg cggggcgata
     4141 gaaaaccact ggtccttcat cgcttacaat gtggcagctg cgaaaggctc gccagaacgc
     4201 gcgaaagatg gattcatctg actttctgta cagatctccg aggaacggag ccatgtgaga
     4261 agtagcttcg cgcaatttat agtgagggat tccaatgaac aaatgatgca ccatgtgagt
     4321 gccaatattg tggtgaaacc attcaaaaat tccgtacgag cgatccacgg aagaaagatt
     4381 gccgcgcacg aaattccagg cttccccgcg ataccaggga acatccggag cggtgtgatg
     4441 aaggaatgtg accaggtcca gccacaccac gaatacaaag tacggtgcaa tgtagaactt
     4501 tgcaagaaat acgattccga atttccatcc gaccacacac aggccagcaa agaagattcc
     4561 ccagagaaga gtgctcgtca gcacatccca tttttcagaa gggcgaaaca gatcactgcc
     4621 gggaaggaag tgagaaccgt cgcgaccagg ggatctgcga aacagataca gcggaaaaag
     4681 gaatagaaat acatagtatc gcaagatgcg aatggacaga gtcatgtgtg aatactgagt
     4741 ttcagtgact ggataccacg tttcatcttt gtccacatca ccagtatgct ggtggtgagt
     4801 gcgatgactg attctccaac catggtaggg cactagaata ggcgtgtgac tcaaatggcc
     4861 gattagattg ttcaaccact tgatgtgcga gaaagaacca tgcccacaat catgacccac
     4921 aacgaacaaa gcccagaaca tggttccctg gagaaaccag tagactggat agaaatacca
     4981 gctgtccaga tagagagcga gagaagcaag gccggcgatg gtgctgatgt cgaagaagaa
     5041 atagaacagg gacttccata cggacggttg aaaacagtga ttgggaatgc ttgcgcgaat
     5101 ttgagagatc gatgggacta cgggtggctg cattatgatc cttcgagttc gaccaggttc
     5161 agatccggtg acaccagatc tccaaccttg cagtgaacgg cctttacttt gccgctcaaa
     5221 gtcgctttga ttgcgttttc cattttcatt gcttccacaa ttacaatggt ctgatccttc
     5281 tgaactgaat cgcccacggc cacttgcacg gcaatgactt ttcccggcat aggacttctg
     5341 tgaagattcg atgtgtttgt ttcaaggtct gcaggagaaa cagacttcat tcgaaaggtc
     5401 tggcctgcga tatgaacata gacttcgtcg cccgcgaagt agaatcgtcc tttcgtttcg
     5461 cccgcggctg tctgaatgga aaaagtatct ccattcaaaa tctgtgctgt ttctacaggc
     5521 agctttatgt ctggtttgcc ttcttccgag attccgatgt agaatccttc gccctgtttc
     5581 tgcagctcga ttgtaaaggg gattccgtcc tggacgaagg tcttgatcat gttcgtaact
     5641 cgcacggcct cggaggctgt cgactcaatc atgaaatgaa ttgcgcggct gcaagcccgc
     5701 agtaaccggg tctgtggaca atagcctttc agaacaat
//
LOCUS       DAOQJL010000211         5734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8045_length_5734_cov_4.206198, whole genome shotgun sequence.
ACCESSION   DAOQJL010000211 DAOQJL010000000
VERSION     DAOQJL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_5734_cov_4.206198"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..943
                     /locus_tag="PLG78_10325"
     CDS             92..943
                     /locus_tag="PLG78_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE23556.1"
                     /translation="MPSNAKLLEIIHLQTEIAKLGIDLSQVMALVVDRTIPLVRADGA
                     AIELAEGEDMVYRAVSGNAKPFLGLRIKRNGSLSGLCVQTGEVLLCEDSETDPRVDRQ
                     TTRKIGLRSMIVMPLRYRDATVGVLKVMSAEAHKFQKEDEMILGLLSEVVAAAMFHAT
                     RLESDDLLHKATHDALTDLANRSLFMDRLRNILSSSSNQAGLLMIDMDGLKTINDSLG
                     HRADELSCLLSENSLLKDRPFPFMQASAGQAFQRILWIWRNSLKPLTVECMRPSEVID
                     RTPHPIP"
     gene            complement(989..2602)
                     /locus_tag="PLG78_10330"
     CDS             complement(989..2602)
                     /locus_tag="PLG78_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE23557.1"
                     /translation="MSFVGTKYKQEEVDSHYDAVIIGSGMGSLSTAALLSKAGKRVLV
                     LEKHYTAGGFTHSYTRKGYEWDVGVHYIGEVHKRLSPLRLLFDLVTDGKLEWSYMGDT
                     YDSVFFGEERYDFMAGEEQFMANLLRHFPGEKDTLKNYIKLIKSANRLAIPFYVSQKI
                     FPDAVGKMASSLSDSILAKYVRRTTGEVLADLTGNKKLRGVLTAQWGNYGLPPAQSSF
                     MIHAAVAHHYLDGGNFPAGGASQIARTIEKVIQKPGGKILVNAPVSQILVNGSRAMGV
                     RLENGHEILADRVISGAGYFNTFGKLLSTETAEKVGGGSKLKTVRPSIGHVCLYTGMK
                     HDAEELKLPMGNLWVYQDYDHDASLARFLEKPNLDFPLVYISFPSAKDPLWKTNHPGR
                     STIDVIAPMPYAWFEKWKGTEWNKRGDEYENYKESISKKLLEKIYKYVPQAEGKISYY
                     ELSTPLSTEHFGNYSRGELYGIDHNVGRFEQNWMKPQTQIENLYVTGQDILFCGVASA
                     LSSGVMTAASILGSEIGSIVPGFANHFRS"
     gene            2721..3308
                     /locus_tag="PLG78_10335"
     CDS             2721..3308
                     /locus_tag="PLG78_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE23558.1"
                     /translation="MKKPYHHGNLKEELIAAGISVLKKEGTGGLRLREVAGLVGVSHA
                     SLYRHFTNKEELLAAIAEQGFRGLIENVELYKKRAGSNFKSQFIESGRAYVDFAASHK
                     EQFHIMFSGIIPDRKKHPALALAAEESFGQLVQIVEECREAGVIPSHRDRKVHTLAAW
                     SMLHGIATLLIEGQIPGGRSRTRAITRSLLILLTV"
     gene            3394..3882
                     /locus_tag="PLG78_10340"
     CDS             3394..3882
                     /locus_tag="PLG78_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23559.1"
                     /translation="MGATMKIRSFLNIAALSLAFAFSTASLRAEPVVLTEDVLKRYMA
                     IFPSYMRITLELDGTSKMREGAAKEAKVTELIEKRNALLTANGWEDFSEYMEVDARVV
                     RAMTPLGILEKLKNASEPDRKKAEDTVAAQLQDYTPEEIALVKKNLVGLTKMRRDALA
                     GR"
     gene            3971..5080
                     /locus_tag="PLG78_10345"
     CDS             3971..5080
                     /locus_tag="PLG78_10345"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE23560.1"
                     /translation="MRPWIRACFISLLSSLAAQCNQSSKCKEADASCNVLPLLLYSKV
                     AVATNLYVVNATSGSLSEYSIDRGTGLLSSVGTATGMAGTNGVQVDPLGRFVFVAAST
                     TPRIHRFAIDTTGALSVLGTTTPNSTFPIGLCADPTGAYLFSVVAGNGTVEGFSVSSN
                     SVLTSTGTALAAAGGISLRIHPSGRFMYTTNSGAANISAYSLSVGAPTNILTTASGGT
                     SPQGIALDPTGNFLYVANSGGTPNIAIFSINQSTGIPTLQTTINSTSVYGIVVDPTGQ
                     YAYTTNQGASNTVSTYRRDSAGLLTLVGTVAAGTAPAFLAIDPQSKFLYVSNNNTATQ
                     YVSQYRIVSGLPVSIGTIAAGDGGYFVDLLVKYFY"
     gene            complement(5095..>5734)
                     /locus_tag="PLG78_10350"
     CDS             complement(5095..>5734)
                     /locus_tag="PLG78_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE23561.1"
                     /translation="VWGAVYLAGILYMLSAGRIIPFHSLFFYGQTAFFPFDALFFILS
                     LYRRYRTMESARLDLEREMHSTLAKLASQSRERSAKKKVGYSRSKLSGVNVGKILESL
                     DHSLRTEKLFKKEDLSLAELASHIGITPHQLSEICNAQLNTSFPKLLAYHRVLEAKEL
                     MANSDLNILQIAFDSGFASKSSFNIEFKRVTGLTPREYRARPDVRHDFASAV"
BASE COUNT         1441 a         1504 c         1394 g         1395 t
ORIGIN      
        1 tcgccttcgc cttaccctcg ctaaatatta aagagagtcg ttgactacca aaagtgccca
       61 aagagaaaga cgtattcaac gggacaagcg aatgccgtct aacgcgaagt tactggaaat
      121 cattcacctc caaacagaaa tcgccaagct aggaatcgac ctgagccagg tgatggccct
      181 cgtggtagat agaactatcc cgctcgtcag ggcagacggc gcggccattg aactggcaga
      241 aggcgaagat atggtctatc gcgccgtatc gggaaacgcg aaaccatttc tcggtttgcg
      301 aatcaagcgc aacggaagcc tatcgggcct gtgcgtacaa acgggtgaag tcttattgtg
      361 cgaagactcc gaaacagacc cgagggtgga tcgccagacc actcgaaaga ttgggttacg
      421 atccatgatc gttatgccgc tgcgataccg cgatgcaaca gtcggtgttt tgaaagtcat
      481 gtccgcagaa gctcacaagt tccagaaaga agacgaaatg attctgggtt tgctgtctga
      541 agtcgtggcc gcggccatgt ttcatgcaac tcgcctcgaa agcgacgatc tcttacacaa
      601 agccacacac gacgcattga cggatctggc caaccgttct ctgttcatgg atcggctgcg
      661 aaatatcttg agctcgagta gcaatcaagc aggccttctc atgatcgaca tggatgggct
      721 caagacgatc aacgattctc ttgggcaccg tgccgacgaa ttgagttgtc ttttgagcga
      781 gaattcactt ttgaaggaca gaccattccc attcatgcaa gcatcggcgg ggcaagcttt
      841 ccagaggatt ctatggatct ggagaaactc attgaaaccg ctgacagtag aatgtatgcg
      901 gccaagcgaa gtcatagaca gaacgccgca tccaatcccc tgaaccaacc ttcgaaacga
      961 aacaatccag actaactccc tggacttctc aggaccggaa gtgatttgca aatccgggaa
     1021 caatcgatcc gatctcagaa ccaagaatgc tcgcggcagt catgactcct gaactcagag
     1081 cgctggctac tccgcaaaac agaatgtcct gtcccgttac atatagattc tcaatctgag
     1141 tttgaggttt catccagttt tgttcaaacc ttcccacatt gtgatcaatt ccgtaaagct
     1201 cgcctctgga gtaatttcca aaatgctctg tggacagagg tgtggataat tcgtagtagc
     1261 tgatcttacc ttccgcctgc gggacatatt tataaatttt ttcaagcaat ttctttgaaa
     1321 tggattcttt gtagttttcg tattcatctc cccgcttgtt ccattccgtt cctttccatt
     1381 tttcaaacca ggcatacggc atgggtgcaa tcacatcgat ggtggaacgg cccggatgat
     1441 ttgttttcca gagcggatct ttcgcactcg gaaaagaaat atagacgagg gggaaatcca
     1501 gatttggctt ttccaggaat cgggcgaggc tcgcgtcgtg atcgtagtcc tggtagaccc
     1561 agagattgcc catgggcaat ttcaattctt cggcgtcatg cttcattccg gtgtagaggc
     1621 atacgtgacc gatcgacgga cgaaccgtct tcaatttgga gccaccacct accttctctg
     1681 cggtttccgt ggaaaggagc ttaccgaatg tattgaaata tccagcgccg ctgatcactc
     1741 ggtctgccag aatctcatga ccattttcca ggcgaacccc catcgcgcgg ctgccgttca
     1801 caagaatctg agatacaggt gcattcacca gaatcttccc gcccggtttc tggattacct
     1861 tttcgatggt gcgagcgatc tgagatgcgc ctcccgccgg aaaatttcct ccatccaggt
     1921 agtggtgagc cactgccgca tgaatcatga agctagactg tgctggaggc aaaccgtagt
     1981 tcccccattg cgcagtgaga acccctcgga gttttttatt gccagtcaaa tctgcgagca
     2041 cttcgccggt ggttcgtcgc acgtatttcg caagaatgga atcagataga ctcgatgcca
     2101 tcttgcccac tgcgtccgga aaaatcttct ggctgacgta aaatggaatc gcaagtcgat
     2161 tggcgctctt gatcagcttg atgtaattct tgagtgtgtc tttttccccg ggaaaatgac
     2221 ggagcaaatt ggccataaac tgttcttcgc ctgccatgaa gtcataccgt tcttcaccga
     2281 agaaaacgct gtcgtacgta tctcccatat aagaccattc cagcttaccg tccgttacca
     2341 ggtcaaacag cagtctaagt ggagagagtc ttttgtgaac ttctccaatg taatgaactc
     2401 ccacgtccca ctcgtaacct ttgcgcgtgt aactgtgagt gaatcctccg gcagtgtagt
     2461 gcttttccag cacaagcact cgcttccccg ccttggacaa aagcgccgcc gtggacaaac
     2521 tgcccatgcc tgaaccaatg atcacagcat cgtagtgcga atccacttct tcctgcttgt
     2581 atttcgttcc aacaaatgac atagggcctc cctgtgctcg ccagcctgac attgcacaat
     2641 gtaaccattg gtaacattga tgtttcccgt ggcaacattt ttaattgtaa ttttccctgg
     2701 cgaccgactg ctggtttgag atgaaaaagc cttaccacca cggaaacctc aaagaagaac
     2761 tcattgctgc cggtatttct gtgctaaaaa aggagggaac cgggggcctg cgtctgcgtg
     2821 aagtggctgg ccttgtgggt gtaagccacg cttctcttta caggcacttt acaaataagg
     2881 aagaactcct ggccgccatc gcagaacagg ggtttcgcgg gctcattgaa aacgtggagc
     2941 tctacaagaa gcgcgccggc tccaatttca aatcgcagtt catcgagtct ggccgcgctt
     3001 atgtggattt tgccgcgtca cacaaggaac agtttcatat catgttcagc ggaatcattc
     3061 cggatcgaaa aaagcacccg gccctggcgc tcgcggcgga agaatcgttt gggcagctgg
     3121 tgcagattgt ggaagaatgc agagaggcag gagtgattcc ctcccacaga gatagaaaag
     3181 tgcacactct ggctgcctgg tccatgcttc atggcatcgc cactctcttg atcgaaggcc
     3241 agattccagg gggcagatcg cgcactcgag ccatcacacg atctcttctg attttgttga
     3301 ctgtgtgatt cgcacagacg cagagcctta ctttttccct ccccatcccc cgtttttatt
     3361 gacacttctt tctattggat gcgtctctca tctgtgggtg caacaatgaa gattcgatcg
     3421 tttctcaaca tcgcggctct gtctctggcg tttgcctttt caacggcgtc tcttcgcgcg
     3481 gagccagtcg ttctgacgga agacgttctg aaaagataca tggcgatttt tcccagctat
     3541 atgagaatca ctctggagct agacggtacc tctaaaatgc gggaaggtgc agcaaaagaa
     3601 gcaaaggtaa cagaattgat agagaagagg aatgcccttc tcactgcgaa tggatgggag
     3661 gatttctccg agtacatgga agtggacgcc cgcgttgtgc gcgccatgac tccgctgggc
     3721 atcctggaaa aactcaagaa tgcatcagag cctgacagaa agaaagcgga agatacagtt
     3781 gcagcacagc ttcaggatta cactccggaa gaaattgctc tggtaaaaaa gaacctggta
     3841 ggccttacaa agatgcggcg tgatgctctg gctggaaggt aaagcgcgca taatcttctc
     3901 tccataataa aaaggtcgcg gattatagac cgcggcgaca ggcgccagat gatccgctat
     3961 catcctgact atgcgaccat ggatcagggc ctgtttcatc tctcttttgt ccagcctggc
     4021 ggcccaatgc aaccagagct ccaaatgcaa ggaggcagat gcaagttgca acgtccttcc
     4081 cctgctgctt tattcaaagg ttgccgtcgc aaccaatctc tacgttgtaa acgccacgtc
     4141 cggttcactt tcagagtact ccattgacag gggcacggga ctgctctcct ccgttggaac
     4201 cgcgacaggt atggcaggca cgaacggtgt gcaggtggat ccgctcggtc gttttgtctt
     4261 cgtggccgcc tcgaccacgc ccaggatcca tcgtttcgcc atcgatacga cgggtgcgct
     4321 ctcggtcctt ggcacaacga cacccaattc aacatttcct attggcctct gcgctgatcc
     4381 cacgggcgcc tatttattca gcgtggtcgc cgggaatgga acagtggaag gtttcagtgt
     4441 gagcagtaat tcagtgctca caagtacggg aaccgcattg gccgcagcgg gcggaatctc
     4501 tctgcgcatc catccatccg gccgatttat gtatacgacg aactcaggag ccgccaatat
     4561 ttccgcttac tcgcttagcg tcggagctcc aacaaacatc cttacaaccg cgtccggagg
     4621 gacttcaccg caggggatcg cgctggatcc taccggcaat tttctatatg tcgcaaattc
     4681 gggcggcaca cccaacatcg ccatcttctc tatcaatcag agcacaggca ttccgaccct
     4741 gcagactact atcaactcaa cgagcgtcta cggtatcgta gtcgatccaa cgggtcagta
     4801 cgcgtacaca accaatcagg gtgcttccaa tactgtgtcc acatacagac gcgacagtgc
     4861 gggacttctc acgttagtcg gcactgtagc ggccggaacg gcaccggcat ttcttgcgat
     4921 cgatccacag agcaaatttt tgtatgtgag caacaacaat actgcaacgc agtacgtttc
     4981 acaatatcga atcgtttcag gccttcctgt ttccatagga accatcgcag cgggagacgg
     5041 cgggtatttt gtagatttgc tggtgaaata tttctactga gtaaaggatg attgtcacac
     5101 ggctgaagca aagtcatgcc gcacatccgg ccttgccctg tattcgcgcg gagtcagccc
     5161 cgtgactcgc ttgaactcta tattgaaaga tgatttagat gcgaaaccag aatcaaaagc
     5221 aatctgcaga atattcagat cactgtttgc catcagctct tttgcttcca gaactctatg
     5281 atacgccagc aacttaggaa aactggtatt caactgagca ttgcaaatct cagacaactg
     5341 atggggtgtg atgccaatgt ggctggcaag ttctgcaaga gagagatcct ccttcttaaa
     5401 tagcttctcc gttcgcaaag agtgatccag actttctaga atttttccta cattcacacc
     5461 ggatagtttg ctcctggaat aaccgacttt cttcttcgcg gatcgctcgc gagactgaga
     5521 agcaagcttc gcaagagtag aatgcatctc gcgctccaga tccagacgtg cactctccat
     5581 cgttctgtat ctcctgtaca aactgagtat aaagaagaga gcatcaaacg gaaaaaatgc
     5641 agtttgaccg tagaaaaaaa gcgaatgaaa cgggatgatg cgtcctgcgg aaagcatgta
     5701 tagaattcct gccagataca ctgcccccca gacg
//
LOCUS       DAOQJL010000212         5659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8220_length_5659_cov_4.477159, whole genome shotgun sequence.
ACCESSION   DAOQJL010000212 DAOQJL010000000
VERSION     DAOQJL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_5659_cov_4.477159"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLG78_10355"
     CDS             <1..1393
                     /locus_tag="PLG78_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE23562.1"
                     /translation="VVASYPTKGLSGIDGSQGIWLLFDREMDQQKTQSAFTLGSAGGG
                     TTGGFLWEGTRMIYNPRSGLTGSDEFTMTLSKSAEASTGVNIQQDYIVRFFAQADTTH
                     PTLTSSNPANGATNVATNQTITLNFSKPIDFSTISSGISISPAIVTTIIQNQAKDQVI
                     ITPSSNLTYGTTYTVNLTTALKDVFGNSLQNGSTISFVVGSASSPPALTSATAGVLLT
                     NGINTTGIDRNSPIVLQFSAAMNPVTTEAAISLSPAASWIKTWNGASDQLTLTFPSGL
                     SSQSNYTLSVSNSASDSFGNLMSTNYSFPFYTDAAVSTRPVVTSVRQEGSSTPGTCAG
                     ASGSGIYSALTDYATLDLAQQADADPGPLTTCILQIRIDFSGTMVRNSLVTSTSFQRI
                     FDASSGNVQIYGIVTPGPTSGNSMTVQLYGNPWPSAPGIPTYRLKILGGPSGAIDVNG
                     NTLASDYVIYLTF"
     gene            1393..4587
                     /locus_tag="PLG78_10360"
     CDS             1393..4587
                     /locus_tag="PLG78_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23563.1"
                     /translation="MLRFLVNRPIATSMIYAAILLIGFISLGRLRVSLFPDIQIPQLT
                     ILTAYPNVAPDEMENLVTRPIEDAVSSVSGVRRVTSKSQEGLSIVEVFFDWNTSLDLA
                     IVNTRQKIDLAKSVLPQDAEKSIIVRSDPSADPIITLIARPVDLPEMQLRDYIENDVR
                     PFIERIDGVASLSVLGGSKREIQVKVDAQRLHSIGLSLEDLRGALESSNFSFPAGTVR
                     KGDRDLTVRVQGDFQSVEQIGETVINVSSSGAPVFLRQVAEIENGFKERRGSAFYNGK
                     PAVVVGIKKEPDANTIQTAANIRAGLAEINARFGKKLKLEIVQDKSDFVLSAIANVRT
                     EALSGGIIAVIVLFLFLKDVWPSLIISITIPISIITTFACMYIKGLSLNIMSLGGLAL
                     GVGMLLDSAIVVIESITLEREQNPDDSLIDTAVRGTQQVVGSVISAAGTTIVVFVPII
                     FVPGIAGQVFKDFAFTITFSLLSSLLCSLTLVPVLFSLPVYKVTWMRRAFEKINHISK
                     PAFGYAEKFTSLASRLYDRSIDYSLARPARILLISLGVGVVGIALLCFLPRSLFPDVD
                     QGVVEAEVEMPPGTTLAENEALQQKIHGFLSKNNLAAHAITTLGFESDDLASQVKGIK
                     HPNYSRSIIYIDRDRMTSREFNETLSGGLSNFANIKTTFRIKGEALQELLGETSPVIV
                     AEVEHRSRTRAREAALALRESIQKTKGIVNISSTAGATEPEVKVFVDREKIAAQGLTI
                     ESIARTIHSAVNGTIATGYREGDHEIGVRLRLREEDRNSLDHVRRLLVPVGDGKQMEV
                     GTFIRPQEGTGHSAVLRENQRRVEQIRVEYEKSERKTVFAELEQAISNTRASYAKEPD
                     ENRPQLRLREDNEETSESLAGLVYAFAISAVLIYMLLAGQFESLIHPITLALIIPLML
                     FGVSTALLITGHGLNISSAIGIILLAGIVVDSATVLYEYIEQQRGDAGHDGSDIALLP
                     EILKKCGHARIRAILLTSLTNMLGSLPSAGLASSGNLQAPMAVAIVGGLIVATVLTLV
                     AFPTMFLVVETFRHRVRQNGTLLAIKSLTARPAA"
     gene            4584..>5659
                     /locus_tag="PLG78_10365"
     CDS             4584..>5659
                     /locus_tag="PLG78_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23564.1"
                     /translation="MNRFLGFFPQRPVLCLMLTLCGCFLGAISLLHLPVGLMPNLANP
                     GITIVTRYPGVSAEKIHQMITIPIERSVSDLGNIEEILSVSSEGESKINLLFSNEADI
                     KIKMVEASERIDLASARFPRDVNAPYIVQYDPSDRPVFVVSFSSSHMDLKALREHIDR
                     KVKLKFERVEGVSEVFVGGGFEREIQIAVDPGKLSTSGTTISGVQSSIAEANTYVPGG
                     RIGIPRETSLYTDARLTSTTSLAEVPVRGQKGSWLPLDRLAHVTDSFRDPDSVARTNG
                     EERVTIFIQKSGKANTLSLTEACETIVSGLGGEEFETHIVHNQGEHIRRAIRRVSLEC
                     IIGGIAALIVLQAFLQKRSNSLLIG"
BASE COUNT         1415 a         1521 c         1367 g         1356 t
ORIGIN      
        1 ggtagtggcg tcttacccga cgaagggcct gagcggcatt gacggttcgc agggaatctg
       61 gctccttttt gatcgcgaga tggatcagca aaaaacgcag tccgccttca cacttggaag
      121 tgcgggaggc ggcacaaccg gcggctttct ctgggaagga acgcggatga tctacaaccc
      181 gcgatcgggg cttaccggtt cagacgaatt cacaatgact ctttcaaagt cggcagaggc
      241 ctccacaggc gtaaacatcc agcaggacta cattgttcgc ttcttcgcgc aggcagacac
      301 aactcatcca actcttacgt cttcgaatcc tgcaaacggt gctacgaatg tagccacaaa
      361 ccagacaatc actctcaatt tttctaaacc tatagatttc tcaactatct caagcggaat
      421 ttcgatctca cccgccatcg taaccaccat aatacagaac caggcaaaag atcaggtcat
      481 catcactccg tcctcgaacc tgacatacgg cactacctac acagtgaacc tgacaacggc
      541 tttgaaagac gtttttggga attctctgca gaacgggtct acgatttcgt ttgtagtagg
      601 aagtgcttct tcgcctcctg cactcacttc tgcgacggca ggagtactgc tgacaaacgg
      661 aatcaatacc accggaatcg acagaaactc acccattgtc ctccagttca gcgccgccat
      721 gaatccggtt acgaccgagg ctgccatctc cctttctcct gctgcctcct ggatcaaaac
      781 atggaacggg gcgtcagacc agctgactct gacttttccg tccggccttt cttctcaatc
      841 caactacaca ctttccgttt caaactcagc ttccgattct ttcggaaact tgatgagtac
      901 caactattct ttcccgttct acacggatgc agcggtgtcc acgcgaccgg tcgtcacttc
      961 tgtaagacag gaaggttctt ctacgcctgg aacctgcgcg ggtgcttctg gcagtggaat
     1021 ctatagcgca ctgacggact acgcaactct cgatctggcg caacaggcag acgcagatcc
     1081 gggtccactc acaacctgca ttctccagat tcgcattgat tttagcggca ccatggtgcg
     1141 aaattcactg gtcacttcaa cgagtttcca gagaatcttc gacgcttcga gcggcaatgt
     1201 tcaaatttac ggaatcgtca cacccggacc gacgagcggc aacagcatga cggttcaact
     1261 ctacggcaat ccatggccat ctgctccggg aattcccacg tacagactga agatactggg
     1321 aggaccgtca ggcgccattg atgtcaatgg aaacactctg gcttctgact acgtcattta
     1381 ccttacattc tgatgcttcg cttcctcgta aatcggccca tagccacctc gatgatctat
     1441 gcggctattc tactcatcgg ttttatttct ctggggcgac tgagagtaag cctgtttccg
     1501 gatattcaga ttccgcagct caccatactc actgcctacc cgaacgttgc tccagacgaa
     1561 atggaaaatc tggtgactcg tccaatcgaa gacgctgtga gctctgtgtc cggagttcgc
     1621 cgtgtaacgt caaaatccca ggaaggtctt tcgattgtag aagttttctt tgattggaac
     1681 acttctcttg atctggccat tgtgaataca cgccagaaaa tcgaccttgc caaatcagtt
     1741 cttcctcagg atgcagaaaa atcgatcata gttcgttcgg atccgtccgc agatccaatc
     1801 atcactctca tcgcgcgccc ggtggacctg ccagaaatgc agctgcgcga ttacatcgag
     1861 aatgatgtaa gacctttcat cgaacgcatc gatggagtcg cttccctttc tgtgctcggt
     1921 ggatccaaac gagaaatcca ggtcaaagtc gacgcacaaa ggctgcattc gattggcctt
     1981 tcgctcgagg acctgagggg tgctctcgaa tcgtctaact tcagttttcc ggccggcacg
     2041 gtgcgcaaag gagaccgcga cctgacagtg cgcgtgcagg gagattttca gagtgtagaa
     2101 caaatcgggg aaacagtgat caacgtatct tcttctggtg cgccagtgtt tctgcgccag
     2161 gtagcagaaa ttgaaaacgg attcaaagag agacgcggtt ccgccttcta caacggcaaa
     2221 cctgcagtcg tagttgggat caagaaagaa ccggatgcaa atacgatcca gactgccgca
     2281 aacattcgcg cgggtctggc tgaaatcaat gcgcgatttg gaaagaagct gaagctggaa
     2341 attgttcagg ataaatccga ttttgttctg tctgccattg caaacgtgag aacagaggcg
     2401 ctctccggag gcatcatcgc agtgatcgtt ctgtttttgt ttctaaagga tgtctggcct
     2461 tctctcatca tttcgatcac gattccaatt tcaattatca ccacgttcgc atgcatgtac
     2521 atcaaagggc tttcgctgaa catcatgagc ctgggaggcc tggcactcgg ggtgggcatg
     2581 ctgctcgaca gtgcgattgt agtgatcgaa tcgatcacac tcgaacgaga acaaaatcca
     2641 gatgattcac tgatagatac tgcagtgcgg ggaactcagc aagttgtggg ctctgtcata
     2701 tctgctgcag gcacaacgat cgtggtattt gttcccatta ttttcgttcc agggattgca
     2761 ggacaggtat tcaaggattt cgctttcacg atcacattct ctcttctgag ctcccttctc
     2821 tgttctctga ctctcgttcc cgttcttttt tctctgccag tgtacaaagt aacgtggatg
     2881 aggcgagcat ttgaaaagat caatcacatc tcaaagcctg cctttggtta cgcagagaaa
     2941 ttcacatctc ttgcttcgcg tctctatgat cgctctattg actattccct tgcaagacct
     3001 gcgcggattt tactcatctc actcggagtg ggcgttgttg gaatcgcact cctatgtttt
     3061 cttccgcgaa gccttttccc ggacgtagat cagggtgttg tggaagcaga agtggaaatg
     3121 cctccaggca ctactctggc agaaaatgaa gcattgcagc agaagattca cggctttctg
     3181 agtaaaaata atctggccgc tcatgcgatc actactctcg gatttgaatc cgatgacctt
     3241 gccagccagg taaaaggaat caagcatccg aactactctc gcagcatcat ctacattgat
     3301 cgagatcgca tgacgtctcg agaattcaat gagacgttgt ccggcggtct ttccaatttc
     3361 gcaaatatca agacaacatt caggatcaaa ggagaagcac tgcaagaact gctcggagaa
     3421 acatctcccg tgatcgtcgc agaagtagaa cataggtcgc gaacccgcgc tcgcgaggcg
     3481 gccctggctc tgcgcgaaag cattcaaaaa acaaaaggaa ttgtaaacat ctcatctacc
     3541 gccggtgcaa cggaacctga ggtaaaggtg tttgtggatc gcgagaaaat agcagcccag
     3601 gggctgacca tcgaatcgat cgcacgcacc atccatagcg cggtcaacgg caccatcgca
     3661 accggctacc gcgaagggga ccacgaaatc ggtgtcaggc tcagacttcg cgaagaggac
     3721 agaaacagcc tcgaccatgt gagacgcttg cttgtgcctg tgggcgatgg caagcaaatg
     3781 gaagtgggca ctttcatccg ccctcaagaa ggcacgggtc attctgcagt tcttcgcgag
     3841 aatcagagac gtgtagagca aatccgggtc gaatatgaaa agagcgaacg aaagacagtc
     3901 ttcgcagagc tggaacaggc catctcaaat acccgcgcat cctatgcgaa agagccagat
     3961 gaaaaccgtc cgcaactgcg gctacgcgaa gacaacgaag aaacaagcga atccctggca
     4021 ggtctagtgt atgcgtttgc aatttcagct gtgctcatct acatgttgct cgcaggtcaa
     4081 tttgagtcac tcattcatcc aatcactctg gcactcatca ttccactgat gctattcggt
     4141 gtgtctacag cacttctcat cacaggacac gggctcaata tctcttctgc cattggcatc
     4201 attcttctgg cagggatcgt tgtggattca gccactgttc tctatgagta catagaacag
     4261 cagcgaggtg acgccggaca cgacggatca gacatcgcgc ttttgcctga aattctaaag
     4321 aaatgcggac acgctcgcat ccgtgcgatt ctactcacaa gccttaccaa catgcttggc
     4381 tctcttccat ctgcgggact cgcctcaagt gggaatctcc aggcgcctat ggcagtggcc
     4441 atcgtaggcg gattgatcgt agccacagtg ctcactctgg tcgcattccc gaccatgttt
     4501 cttgtagtag agacgttccg ccacagggtc aggcagaacg gaactcttct ggccatcaaa
     4561 tccctgactg caaggcccgc tgcatgaacc gctttctcgg attctttcca cagaggcctg
     4621 tgttgtgtct gatgctcacg ctgtgcggct gctttctggg cgccatttcg ctcctccatt
     4681 tgcctgtcgg cctcatgcca aatcttgcga atcctggcat aaccattgtt acgcgatacc
     4741 ccggtgtttc cgcagagaag atccatcaaa tgatcaccat tcccattgag agaagcgtaa
     4801 gcgatcttgg gaacatcgag gaaatcctgt ccgtatcaag cgaaggagaa tcaaagatca
     4861 acctgctatt cagcaacgaa gcggacatca aaatcaaaat ggtagaagca agcgagcgca
     4921 ttgatcttgc atccgcaagg tttcctcgtg atgtaaacgc gccctacatt gtgcagtacg
     4981 atccctccga tcgacccgtg tttgttgttt ccttctccag ttcgcacatg gatttgaagg
     5041 cgcttcgcga acacatagac agaaaagtga aactcaaatt cgaaagagtg gaaggagtat
     5101 cggaagtgtt cgtcggaggc ggcttcgaac gcgaaatcca gatagcagtt gatccaggaa
     5161 aactatccac atccggcaca accatctctg gtgtacaatc gtccatcgca gaagcgaaca
     5221 cctatgttcc aggaggaaga attggcatcc ctcgcgagac ttcgctgtac acggatgctc
     5281 gcctgacgtc tacgacatct cttgcggaag ttccagtgcg cggccagaaa ggttcgtggc
     5341 ttcccctcga tcgactcgcc cacgtgacag actcttttcg cgatcctgat tctgttgcca
     5401 gaacaaacgg ggaggagcgc gtcacaatct tcattcagaa atcggggaag gcaaacacac
     5461 tttcgcttac ggaagcgtgc gaaacaatcg tgtcaggcct gggcggagaa gaatttgaaa
     5521 ctcacattgt tcacaaccag ggcgaacaca tccggcgcgc cattcgccgg gtttctctgg
     5581 aatgcatcat cggtggaatc gcagccttga ttgtgctgca ggcctttctg caaaagagaa
     5641 gcaattcgct actcattgg
//
LOCUS       DAOQJL010000213         5652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8237_length_5652_cov_4.289798, whole genome shotgun sequence.
ACCESSION   DAOQJL010000213 DAOQJL010000000
VERSION     DAOQJL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8237_length_5652_cov_4.289798"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLG78_10370"
     CDS             complement(<1..1534)
                     /locus_tag="PLG78_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23565.1"
                     /translation="MRILNIRNGTTFALLALVTTTWYFAAGLLSPSWRDDSLGGNDWD
                     LNFFYTESSALPVRDFAQIPFWNPFYKGGMPLFENPQARTFTPAVFLAPVMGPVRALK
                     FSVFIYFLLGAAGSFYLFRFALSIRSLALVIPVVMVPFCGYVPQHVFAGHANFVSILM
                     LPWCLGAMILFQNTGRFRHVAVFSLSAATMVWDGHIYGFLQLLVFVTIVFSTMAVVQK
                     KPEIFLNAFILLVAACALSAVRLLPEIVYFLKYGAYFRPDHVSLTLSNVLHTFVASSQ
                     DPLLARNWENQEHAWWEYGNYIGLLPFLLLLCALPFLRKSEVPWLVALVLFILLMMGD
                     FHVLSPAHLLSQIPGFSNMRCSGRWGFPVVISLAVLLSICLHRALEKASEYTGMGAIA
                     SVLVICFSLYFCYDIRKNRKPISQTFVSGKSASSLVSRIERSLITVPSLPTYGANSAQ
                     FAGILNFFSVRDGYDMLNSNTSMPAKGEAFYRGEYYLEHGSEVKPQLWTPNRIDFEIE
                     SEK"
     gene            1614..1994
                     /locus_tag="PLG78_10375"
     CDS             1614..1994
                     /locus_tag="PLG78_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE23566.1"
                     /translation="MLSNTVLWSLQGVLCFVFLAAGLLKMLRTREQIMNGGGEWAVNV
                     SARNIRIIGVLEIAAAIGIILPLYFNVMPVLTFFAAVGMIFTMIVAGSLHFKRGEMAL
                     LLVTVATGFFAALVAFGRRGLLGL"
     gene            complement(2078..3790)
                     /locus_tag="PLG78_10380"
     CDS             complement(2078..3790)
                     /locus_tag="PLG78_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNE23567.1"
                     /translation="MTFARRSAWFLGIVSVFCAGLFQIPVNLQGTLVYSATLVVFWWR
                     IRHSKVRSFWQHPLIAVVGGFILAGFLTFTTNGLLVTALHLSIFYLLGDLLLRQFGEG
                     PPESISDYCGRAAKLILLSLKRFFSRSGVVEFSSLKTLREDGTRGRLPLVLFSVLLGF
                     VIFFLLHYLFAAVNDDYRRFMDPLVRWVWSACLKNLCLSVIHAYILFCIFTVERSENK
                     KEERALPIFTAVAITVGAVIVTGVFAFFQTRVVLSSSDLNFHELSKYTQKGFLQLIFA
                     ILLGYLIALLAIYSKQKAGKGNHLIALVIFLMLEMILAASFSAHKLYLLQSAFGLKDQ
                     RILASCGVLFVGFSFLLLFWKIFSPNPNFSGFRAQTFFLGALVVALNWINVDLFTTHT
                     NPIRYYVDKKPYVDYSYLLSNSDDNFAEWPALMRRMKEENPPAPPHGYFWGFESKSGK
                     DAFYIRYAPICSDRPEIRTVKEADGTTLHLPTAHVAFLFKKYGTLAPAEPAKNPVASP
                     NNELDEEDIQPEEIHPSFDLSQALDFNWREYASYKSARANAKTFEEFSLYLDKYCEAP
                     PGRM"
     gene            complement(3818..5215)
                     /gene="xrtN"
                     /locus_tag="PLG78_10385"
     CDS             complement(3818..5215)
                     /gene="xrtN"
                     /locus_tag="PLG78_10385"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase N"
                     /protein_id="HNE23568.1"
                     /translation="MRIRDESQSVSVMKMRGESRLDRLMQGHPQTWLTITAILSLVPF
                     LGTAYALFGGAPQSYLSLYPIGFLILFRSQKVRHRWLPLLFVALSILITLSGILTNRL
                     SMIWVGSFWSFLGVCNLYGVPFAFPAPLLVFAAPPLAAFTSLLGGFSLRLWLTARAVQ
                     ILSLLDVTATAQGNVIQFRGNQFSVDRVCEGMKMAVATALIAMVLARFSDRRGRLAIF
                     LLAVPLWLGANLIRIICLAIFHVPPSSASHELIGLVLFAALVVFPLMLMALLFPVQIQ
                     DTRPQLPFPNPLFGPLSRFVLPIVFILLSAGFLLYERRPLPFAWPESVGISARLTSGD
                     RKFLLQQSSVGGDSRFAVYGLGDSSLILKRDLFAPGTAHDPHICFEAAGFAFQGETEE
                     TIGDIRLKSALVQKDGKSARLFWWYSWNTKRSSSDLEWRVARLRGEDVIQFNLYGFDD
                     AALRNAAQMLLKSEI"
     gene            complement(5199..>5652)
                     /locus_tag="PLG78_10390"
     CDS             complement(5199..>5652)
                     /locus_tag="PLG78_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XrtN system VIT domain-containing protein"
                     /protein_id="HNE23569.1"
                     /translation="LKMLGDRRIQVPRETDRVFPLRASAIELEESAESASRPGSDLLV
                     RMLMYRRIMRTLGQRFFNHDLDNADMVKLARDGMIVSPVSTLIVLESEADYKRFGIEN
                     DPSLLGQSKLEAPGAVPEPHELALLLLILLVIFYTRTRIRTAVHAHSR"
BASE COUNT         1667 a         1421 c         1358 g         1206 t
ORIGIN      
        1 ccttttcgct ttcaatttca aagtcgattc tgtttggagt ccagagctgc ggctttactt
       61 ctgagccgtg ttccagatag tactcccctc gataaaacgc ctctcccttt gccggcatgg
      121 acgtgtttga attgagcatg tcgtaaccat ctcgaacaga aaagaaattc aagatgcctg
      181 caaactgcgc ggagtttgct ccatatgttg gaagagaggg tactgtaatt agagatcttt
      241 cgattcgaga caccagactg gatgcggatt ttccggatac gaaggtttgc gaaatgggct
      301 ttctgttctt gcgtatgtca tagcagaagt acaacgaaaa gcatatcacc agcacgctgg
      361 caatcgctcc catgccagtg tactcgcttg ctttttccag agctcgatgg agacagatgg
      421 aaagtaagac agccagcgaa atgacaacag ggaaacccca tcgtcctgag catcgcatat
      481 tgctgaaccc tgggatctgc gagagtaaat gcgcgggcga aagaacgtga aaatcgccca
      541 tcatcagaag gatgaacaat accagtgcca ccaaccaggg gacttctgat tttcgcaaaa
      601 agggaagtgc gcatagtagc aacaggaaag gcaagaggcc aatatagttt ccatattccc
      661 accacgcgtg ttcctgattt tcccagtttc gcgcaagaag aggatcctgg gaagaggcca
      721 cgaaggtatg caacacgttc gaaagtgtga gtgaaacatg gtcaggcctg aaataagcac
      781 catactttag aaaatatact atttccggaa gtaacctcac agccgaaagc gcgcatgctg
      841 cgaccagcaa aataaatgcg ttcaggaaga tttcaggttt tttttgaaca acggccatcg
      901 tagaaaacac gatcgtcaca aatacaagca gctgaagaaa tccataaatg tgtccatccc
      961 agaccatagt cgccgcactg agagaaaaaa ccgctacatg acgaaatcga cctgtgtttt
     1021 gaaacagaat catggcaccc aaacaccagg gaagcatcag gatcgaaacg aaattcgcat
     1081 gcccggcaaa cacatgctgc ggaacgtatc cgcaaaaagg gaccatcaca acaggaatga
     1141 cgagcgcaag gcttcgaatg gacaatgcaa aacgaaacaa gtagaaactg cccgctgcac
     1201 ctaacaggaa gtagatgaaa acggaaaact tcagagcacg cacggggccc atcaccgggg
     1261 ccaggaaaac agcaggcgta aatgtacgcg cttgcggatt ctcaaacaaa ggcatcccgc
     1321 ccttgtagaa agggttccag aaaggaatct gtgcgaaatc tcggacaggg agagcagaac
     1381 tctccgtgta aaagaaattc aaatcccagt cattgccacc cagggaatca tccctccacg
     1441 aaggcgaaag aagaccggcg gcaaaatacc acgtcgtagt cacaagcgca aggagcgcga
     1501 atgtggtgcc gtttctaatg ttcaggattc tcaagtgatt cttctttcct acgatgtccc
     1561 caccattcaa ggattttcgt tgaccgtctc agccacgtgc gctacaatcc gacatgcttt
     1621 caaacaccgt tctgtggtca cttcagggag ttttgtgttt tgtttttctg gcggcaggcc
     1681 tcttgaaaat gcttcgaacc agagaacaaa tcatgaatgg cgggggagaa tgggccgtaa
     1741 acgtttccgc aagaaacatt cgcatcatcg gtgtcctgga aatcgcagct gccatcggaa
     1801 tcattctgcc gctctacttc aacgtcatgc ccgtactcac cttcttcgcg gccgtaggta
     1861 tgattttcac catgatcgtt gctggatctc tccacttcaa acgcggagaa atggccctac
     1921 tactggtcac tgtagccacc ggattttttg cggcacttgt ggcctttggt cggcgaggac
     1981 tcctgggcct gtaacgcagc gcatgcggga ctacatgcgt cctgggcatt tggagccaaa
     2041 ggtccgacta ttccagaata gacaaacccc gccgggacta cattcgtccc ggtggtgcct
     2101 cgcaatattt gtccagatag aggctaaact cttcaaacgt cttcgcattg gcccgcgcac
     2161 tcttgtacga tgcgtattcg cgccagttaa aatcaagagc ctgactcaga tcaaacgaag
     2221 gatgaatctc ctctggttga atatcctctt cgtctaactc attgttaggt gatgcgacag
     2281 gattcttcgc aggttcggcc ggagcaagcg tcccatattt cttgaagaga aaagccacat
     2341 gcgccgttgg caaatgcaac gttgtaccgt ccgcctcctt tactgtccgt atttcaggtc
     2401 gatcagaaca aatcggagca tagcgaatat agaatgcatc tttcccggat ttgctttcaa
     2461 agccccagaa atatccgtga ggcggagcag gaggattctc ctctttcatc ctccgcatca
     2521 aggccggcca ttcggcaaag ttgtcgtcgg aattagaaag caagtaagaa taatctacat
     2581 aaggcttttt atccacatag tagcgaatcg gattcgtatg agtggtgaac aaatccacgt
     2641 tgatccagtt gagcgcaacc acgagcgcac ccagaaagaa cgtttgagcc cggaagcccg
     2701 aaaaattagg attaggagag aatatcttcc agaatagaag caaaaaagaa aaaccaacaa
     2761 acagaacgcc gcagctcgca agtattctct gatctttcaa accaaatgca gattgaagca
     2821 agtacagttt gtgagctgag aaactggccg ccagaatcat ctccagcatc agaaaaatca
     2881 caagagcaat gagatgattt cccttgcctg ctttttgctt gctatagatg gcaagaagtg
     2941 caatcaaata tcccagcagg atcgcgaaaa tcaactggag aaatcccttc tgagtgtact
     3001 tcgagagttc gtgaaaattc aggtcactcg aagaaagcac taccctcgtc tgaaaaaaag
     3061 caaacactcc agtcacaatc acggccccca ctgtgattgc aacggcggta aagatcggca
     3121 atgcccgctc ttctttttta ttctccgatc tctctacagt gaagatacaa aacagaatgt
     3181 aggcgtgaat cacggagagg caaagattct tcagacacgc agaccatacc cagcgcacaa
     3241 gtggatccat gaatcttctg taatcgtcat tgaccgcggc aaacaagtaa tgcaacagaa
     3301 aaaagatcac gaagcccagc aggactgaaa acaaaacgag aggcagtcta ccgcgagtcc
     3361 catcttcgcg caaagtcttc agagaagaga attcaaccac gccactgcgc gaaaaaaatc
     3421 tctttaagga aaggaggatg agctttgctg cccgcccaca gtagtcggaa atgctttccg
     3481 gtgggccctc tccaaactga cgcagcaaaa gatcgcctaa caggtagaag atactcagat
     3541 gcagagcagt cactagcaaa ccgttcgtgg tgaaggtcag gaatcctgcc agaatgaatc
     3601 cgcccaccac tgcgatcaat gggtgctgcc agaaggaacg aactttcgag tgtctgattc
     3661 tccaccagaa aacaaccaga gtcgcagaat ataccagggt tccttgcaaa ttgaccggga
     3721 tctggaaaag gcccgcacag aacactgaga cgattccaag aaaccaggca gaccggcgtg
     3781 caaaagtcat ttgattttcc atactgttct tctccatcta gatttcggat ttaagaagca
     3841 tttgagcggc gtttcgcaga gccgcgtcgt caaaaccata cagattgaat tgaatgacgt
     3901 cctctccgcg aagccgtgcg acgcgccatt ccagatcaga agaagaacgt ttcgtgttcc
     3961 aggagtacca ccagaaaagt ctggcagatt tgccatcttt ctgcacaagg gcggacttca
     4021 agcgaatgtc gcctatcgtc tcttctgttt ctccttgaaa tgcgaaacct gctgcctcaa
     4081 aacaaatgtg cggatcatgc gcagtgccgg gcgcaaacaa gtccctcttc agaatcaatg
     4141 acgagtcgcc aaggccatag acagcaaaac gagaatctcc ccccacagag ctctgctgca
     4201 aaagaaattt ccgatctccg gaagtcagcc ttgcactgat ccctaccgac tcaggccagg
     4261 cgaacggaag tgggcgcctc tcgtatagaa gaaagccagc tgacaggaga ataaaaacaa
     4321 tgggaagcac gaatcgactc aggggcccga acaaaggatt ggggaaaggg agctgtggtc
     4381 gagtgtcttg tatttggact ggaaagagca gagccatcaa catgagagga aacacaacca
     4441 gtgccgcgaa caaaaccaga ccaatcagtt catgactggc actggatgga ggtacgtgaa
     4501 atatcgccag acaaatgatt cggatgagat tcgcgcccaa ccataaaggc accgccagaa
     4561 ggaaaatagc aaggcggcct cgtctgtcag agaatcgtgc gaggaccatg gcaatcaaag
     4621 cagtggcgac ggccatcttc attccttcgc acactcgatc gacgctgaac tggttaccgc
     4681 gaaattgaat gacatttccc tgtgctgtgg ctgtgacatc gagcaaagag agaatttgaa
     4741 cggcccgggc agtcagccac agcctcaaag agaatccgcc caataaagat gtgaaggcgg
     4801 caagaggagg agcagcaaaa accagaagag gagccggaaa agcaaacgga accccataga
     4861 gattgcagac tcctagaaaa ctccagaagg atccgaccca gatcatactc aatctattcg
     4921 ttagaatgcc gcttaacgtg atcagaatgc tcagtgctac aaacaacaaa ggcagccacc
     4981 tgtgacgaac tttctgactg cgaaaaagaa tcaaaaatcc tattggatat aaactcagat
     5041 aggattgagg agccccgccg aacaaagcat aggcggtacc gaggaatgga acgaggctga
     5101 gaatggctgt gatagtcaac caggtttgtg gatgaccttg catcagacga tccagtctgc
     5161 tttcgccgcg catcttcatg acactcacac tttgcgattc atcgcgaatg cgcatgcacg
     5221 gctgttcgta tgcgcgtcct agtgtagaaa atcaccagca gaatgagcaa tagaagtgct
     5281 agttcgtgag gttcaggcac tgctccggga gcttcgagtt tgctctggcc taaaagagac
     5341 ggatcattct cgattccgaa tcgcttatag tccgcttctg attcgagtac tatcagcgta
     5401 gaaacaggag atacaatcat cccatcgcgc gccagtttca ccatatctgc attgtccaga
     5461 tcgtgattga aaaatctctg acccagagtt cgcatgattc gcctgtacat cagcatccga
     5521 accagcaaat ctgagccagg tctcgaagcc gactcggctg attcttcgag ttcaatcgca
     5581 gatgcccgga gtgggaatac gcgatctgtt tcgcgaggca cctgaattct acgatcgcca
     5641 agcattttca aa
//
LOCUS       DAOQJL010000214         5645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8252_length_5645_cov_4.888909, whole genome shotgun sequence.
ACCESSION   DAOQJL010000214 DAOQJL010000000
VERSION     DAOQJL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8252_length_5645_cov_4.888909"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1627)
                     /gene="mqo"
                     /locus_tag="PLG78_10395"
     CDS             complement(152..1627)
                     /gene="mqo"
                     /locus_tag="PLG78_10395"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HNE23570.1"
                     /translation="MGKPEHSEVVLIGAGIMSATLGMMLKELDPGIRIHVFERLPEAA
                     RESSDAWNNAGTGHSAFCELNYTPQKADGSVDVSKAVKVASSFEMSREFWSHLIEKGR
                     IDSPKRFIHRIPHMSFVWGENVPFLRKRYAAMSASPLFSGMNYSEDWSLLSDWMPLVM
                     QSRAKENVAATRMDIGTDVDFGSLTRAMLNHLLMEGVDLHMEHEVRDIVRHENGQWNL
                     TICDLATQEEFKTRADFVFIGAGGGSLTLLGKSDIPEADGFAGFPVSGQWLKCTNQAV
                     VDMHQAKVYGKASVGSPPMSVPHLDTRMIGGKKALLFGPYAGFTTKFLKKGSYLDLAA
                     SLELENIFPMLSAGMHNLPLTKYLIQQALQTPEDRIEELRVYLPDAKIEDWELAVAGQ
                     RVQVIRADEEKGGVLEFGTEIVSAKDGSLSALLGASPGASTSVSIILEVLQECFPAQM
                     ASNGWKKKLKEMIPTYGENLAGNAALVKKSRARTASLLELD"
     gene            1715..3079
                     /locus_tag="PLG78_10400"
     CDS             1715..3079
                     /locus_tag="PLG78_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE23571.1"
                     /translation="MALQRQLGFITGALVVVASMVGSGVFYLPQIILGLTQNGWVFML
                     TFLVGGLIAITGSLSYAEMATMFPDDGGEYQYLKKTFGFLPAFLTGWISLLVGFTASI
                     TGSALVLKMYFVPLMDQMLGAENIFHNALFMKFFLACIIITLGTLHIIGVKFGSSVQN
                     ALTVIKMTIVISFILAGLYVLKGADFSQALSWVGPKAETSGLLIYSAALINVMYIYSG
                     WNGATYIGGEMKDPQKNLPRALLSAAVLTTVLYLLLNLVFIAAVTPEKMAGNPGFGGM
                     TAARLFSPGVSTLYGAFIVVILLSSISVQLMIGPRVYFKMAEDGVLFRFLSTVPGKSS
                     SPVYAIITQIALSIFYVLVFDDDELFSYMGFALSVFPILTVIGVMYLRRTQPNAVRPF
                     KVPGYPVVPAIFILLSSAMLALSLANAFTSEKKFGYIMALVVVLGGIPIYYVWKKLAA
                     SQKA"
     gene            3046..3684
                     /locus_tag="PLG78_10405"
     CDS             3046..3684
                     /locus_tag="PLG78_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE23572.1"
                     /translation="MEKAGGIAESLILERCLAPTSFLDSDHPAVQEYAHTHSGSGSAL
                     DRAVKLYYSVRDGWKYNPYKVSTDPIKSKASWVLTQTNGYCTTKALLLAALGRSIGIP
                     TRLGFGDVRNHLSSPRLIEYLKSEIFAWHGFTEFYLEGHWVRCTPAFDSALCRKFGVA
                     ALEFDGRTDSLFHPFDGEGRKFMEYIQYRGVYEDLPHEEMFRSLREIYPHLF"
     gene            complement(3676..3888)
                     /locus_tag="PLG78_10410"
     CDS             complement(3676..3888)
                     /locus_tag="PLG78_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23573.1"
                     /translation="MAGTAVKSSRIRLSVSIAASSAVARSTDVVMSTCPFARMMEAFP
                     FFSTWMWRTMQRPRPGDGRLQAKNLE"
     gene            complement(4129..5157)
                     /locus_tag="PLG78_10415"
     CDS             complement(4129..5157)
                     /locus_tag="PLG78_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNE23574.1"
                     /translation="MDKKKIMIAGAAVGVVIIGVIVYFLVAGNSGREKTVALKPENWD
                     APFSTSKEMIDEVFAMFDEEDMPATFDVFLKGLESGKINMVWKLWELRRQCPQGMDRY
                     NCNSRILAYLMRKYPGAGGEKLATLVKRYLEFEEVMSITRMSDDLTMRERYDIMRKKR
                     HEIFQPDEEALVFGMEEAKMDLADKSAAFFKETAGQSGNQRVKAYEEMRKKAMGRYYD
                     EFVKDEPSFDRYETEIALRDTDFSKVNSSEQAALTNSIRVRYFGEAGAARMAAVEKQL
                     AAEDQAEKNYEAAEAKFLKENPNLTGDAKEAALKKLRSQYMGEEEADAYARRKQYEDA
                     MKAMNVTP"
     gene            complement(5255..>5645)
                     /locus_tag="PLG78_10420"
     CDS             complement(5255..>5645)
                     /locus_tag="PLG78_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNE23575.1"
                     /translation="SANALAALSSLTKSGVAAFNAATPNASGVKYYSYSGHMYLLDPI
                     QHPLMALTWPICSAVGLLNGYGADCDGLVPVSSAHWGTWKGEPSVGWFITGVDHLQIS
                     NTLYSGQTWFDVSGFFLGVAKNAKANQ"
BASE COUNT         1353 a         1469 c         1313 g         1510 t
ORIGIN      
        1 cattcagtgg agaatctaaa ccaatcatca acgtgcttgg gccgtggctc tggacagaag
       61 acagcatgcc agtcctgcca gaaagcatac agagcaggat gcactttgtt ccgacagatt
      121 tgaatctgat ctgagtcgca gtcttacaaa atcagtccag ttcaagaaga gaagcagtgc
      181 gagcgcggct ctttttcaca agtgcggcat tgcccgccag gttttcgcca taagtcggaa
      241 tcatttcttt gagctttttc ttccaaccgt tagacgccat ttgtgcagga aaacattctt
      301 ggagtacttc cagaatgata gaaacagatg tagacgcgcc aggggaagct ccgagcaaag
      361 cggaaaggct accgtccttc gcagatacga tctccgtgcc aaactcgagc actccaccct
      421 tttcctcatc cgcccgaatc acctgcactc tctggcctgc cacagcaagc tcccagtctt
      481 ctatcttcgc atccggtaga tagacgcgaa gttcctcgat tctatcttct ggcgtctgca
      541 gagcttgctg gatcagatac ttcgtcagcg gaagattgtg catccccgca gaaagcatgg
      601 ggaaaatatt ctccagttcc agagacgcgg ccagatccag ataggatccc tttttcaaaa
      661 acttggtggt aaatcccgca tacggtccga agagaagcgc tttttttccg ccaatcatcc
      721 gagtgtccag atgaggaaca gacatgggcg gagatccaac agacgcctta ccatacactt
      781 ttgcctggtg catatccaca acggcctgat ttgtgcactt cagccattgt ccgctgactg
      841 gaaatccagc aaagccgtcc gcctccggaa tatctgactt cccgagcaac gtgagcgatc
      901 ctccgcctgc accaatgaat acaaaatctg ctcttgtttt gaattcttcc tgcgtcgcaa
      961 gatcgcatat cgttagattc cattgcccgt tctcatgacg aacaatgtcg cgcacttcgt
     1021 gctccatatg cagatctact ccttccatca gaagatgatt cagcatcgct ctggtaagag
     1081 agccaaaatc aacatctgta ccaatgtcca tcctcgtggc ggcaacgttc tcttttgcgc
     1141 gagactgcat aacaagcggc atccagtccg aaaggagaga ccagtcctca gaataattca
     1201 ttcctgaaaa cagaggagag gcggacatgg ccgcataacg tttccgcaaa aatggaacat
     1261 tttctcccca gacaaaactc atatgtggaa tgcgatgaat aaaacgtttg ggcgaatcta
     1321 tccgtccttt ctcgatcagg tgagaccaga attcgcgcga catttcgaaa gaagacgcaa
     1381 ccttcactgc cttgctcaca tctacactgc catccgcttt ctggggagtg tagttcagct
     1441 cacaaaaggc agaatgcccc gtgcctgcgt tgttccaggc atctgaactt tcgcgcgcag
     1501 cctccggaag tctctcaaat acgtgaattc gaattccggg atcgagttct ttgagcatca
     1561 tcccgagagt agcactcatg attcccgcac cgatcaatac tacttcagaa tgttccggtt
     1621 tgcccatacc tgttgaaagc gacggaatgt agcgtcgtca atccctttcc gaaaagaact
     1681 tgcggcgatg cagtgcaccg ccatatcgga tggaatggca cttcaaagac agctgggatt
     1741 cataaccgga gcactcgtcg tagtcgcaag tatggttgga tctggcgtat tctatcttcc
     1801 ccaaatcatc ctcggactga ctcagaacgg ctgggttttc atgctgacat ttctggttgg
     1861 cggtctcatt gcaattacgg gcagcctttc ctacgcggaa atggccacca tgtttccaga
     1921 cgacggcggg gagtaccagt atctcaagaa aacattcgga tttttaccag cgttcttgac
     1981 cggttggatt tcacttctgg tgggattcac ggcttcgatc accggtagcg cactcgttct
     2041 caagatgtat tttgttccat tgatggacca gatgctgggt gcagagaata tttttcacaa
     2101 tgcgctgttc atgaaattct ttctggcgtg catcatcatc acactgggga ctctgcacat
     2161 catcggagtg aaattcggaa gcagcgtgca gaatgctctc acagtaatca agatgaccat
     2221 cgtgatctca ttcattctcg cgggacttta tgttcttaaa ggcgcagact tctctcaggc
     2281 tctgtcctgg gtgggaccaa aggcggaaac atcagggctt ctgatttatt ctgcagcact
     2341 gatcaacgtg atgtacatct attctggctg gaatggcgcc acctacattg gcggggaaat
     2401 gaaagatcca cagaaaaacc tgcccagagc gctcttgtct gctgcagttc tgacaaccgt
     2461 tctctatttg ctcttgaatc tggtcttcat cgcggcggta actccggaga agatggcagg
     2521 caaccctgga tttggtggca tgacggccgc gcgtctattc agccctggag tttctacact
     2581 ctacggtgcg ttcattgttg tgattctttt gtcttccata tccgttcagc tgatgattgg
     2641 tcctcgcgtt tatttcaaaa tggcggaaga cggtgtactg tttcgattcc tttccactgt
     2701 tccgggcaaa tcatcttctc cggtttacgc gattataact cagatcgctc tgagcatttt
     2761 ctacgtgttg gtcttcgacg atgatgaact cttttcttat atgggcttcg ctctcagcgt
     2821 atttcccatt ctcactgtaa tcggcgtaat gtatctgcgc cgcacccagc caaacgctgt
     2881 aagaccgttc aaagttccag gataccccgt ggttccggcc attttcattt tgctttcttc
     2941 cgcaatgctc gcactttccc ttgcaaacgc gtttaccagc gaaaagaaat tcggatacat
     3001 catggcactc gttgtggttc tcggtggcat tcctatttac tacgtatgga aaaagctggc
     3061 ggcatcgcag aaagcctgat tctcgagcgc tgccttgcgc cgacttcatt cctggactcg
     3121 gatcatcccg cagttcagga atatgcgcac actcattctg gaagtggatc ggcgctcgat
     3181 cgcgcagtga agctttacta cagtgtgcgc gatggctgga aatacaatcc gtacaaagta
     3241 agcacagatc ctatcaaatc gaaagcaagc tgggtgttga ctcagacgaa cggatattgc
     3301 accacaaaag cccttctgct cgcagctctt ggccgttcga ttggaattcc tacacggctt
     3361 ggttttggag acgtgcgaaa tcacctttcg agtcccaggc tgattgaata tctaaagagc
     3421 gaaatctttg cctggcatgg ctttactgaa ttctacctgg aaggccactg ggttcgctgc
     3481 actccagcgt ttgattcggc actgtgccgc aagtttggtg tagcagctct tgaattcgat
     3541 ggaagaacag acagtctctt tcatcctttt gacggcgaag gcagaaagtt tatggaatat
     3601 atacaatata gaggtgtata cgaagacctc cctcacgagg agatgtttcg gtctcttcgc
     3661 gaaatctatc ctcacctatt ctaaattttt tgcttgcagc cgcccgtcac cgggacgggg
     3721 tctttgcatg gttctccaca tccaggtaga aaagaaaggg aacgcttcca tcattcgcgc
     3781 gaaaggacag gtagacatga ccacatccgt ggatcttgcc acagcactcg aagccgcgat
     3841 ggaaacagac aatctgattc tgctggattt tacagcagtg ccggccattg actcatccgg
     3901 gattggagcg gttgtgcgaa tcgctgaaaa aataaaaatg agcggcaaaa agctctgcat
     3961 ctttggtctt tctaatcagc tcaagaaatt gttctgggtt ttgaaactcg ataagaacct
     4021 ggcgattttt gaaacagaat cgcaggcaat ggcgggacaa actaaaaaag cggcctctgt
     4081 tcaagaccgc tttctgcttc tcgacgcgga gatgctctta aggcgtcctc aaggagtcac
     4141 attcattgcc ttcatggcgt cctcatactg ctttctacga gcatacgcat ctgcctcttc
     4201 ctctcccatg tattgcgaac gcagcttctt gagggcggct tcctttgcgt ccccggtcag
     4261 atttggattt tccttcaaga actttgcttc cgcagcttcg taattctttt ccgcctgatc
     4321 ttccgccgcg agttgctttt ccactgcagc catgcgtgcg gcacctgcct ctccgaagta
     4381 gcgcactcga attgaattag ttaaagcggc ctgttcggaa gaattcactt tgctgaaatc
     4441 cgtgtctctc aatgcaattt cagtttcgta acggtcaaac gaaggctcat ctttcacaaa
     4501 ctcatcgtag tagcgaccca tcgctttctt gcgcatctct tcgtatgctt tgaccctttg
     4561 attgccggat tgaccggcag tttctttgaa aaaggcggca gacttgtctg ccagatccat
     4621 tttcgcttct tccattccaa aaaccagagc ctcttcatct ggctgaaaaa tttcgtggcg
     4681 ctttttgcgc atgatgtcgt atctttcgcg catggtcaga tcgtctgaca ttcgcgtgat
     4741 gctcatcact tcttcaaact caagatatct cttcacaagg gtggccagct tttctccgcc
     4801 agcgcccgga tatttacgca tgagatacgc gagaatcctg ctgttgcaat tgtaacgatc
     4861 catcccctga ggacactgtc tgcgcagttc ccatagtttc cagaccatgt tgattttccc
     4921 ggattcgaga cccttcagga aaacatcaaa tgtggctggc atgtcttctt catcgaacat
     4981 ggcgaatacc tcatcgatca tttctttcga agtagaaaaa ggggcatccc agttctctgg
     5041 tttcagtgcg acggtctttt cgcgccctga gtttccagct acaaggaaat atactataac
     5101 acctataata accacgccga ctgcggcgcc cgcgatcatg attttctttt tgtccatatg
     5161 ctttacgttt cagagttgtt ggaaggccgt gcaagtaaaa aagaaaaagc ggacgcaagc
     5221 gcccgctttc ccaaaatgat tctggtctaa agtcttactg attcgctttt gcgtttttcg
     5281 caacgcccag gaagaatcca gatacgtcga accaggtttg tccagaatac agagtgtttg
     5341 aaatctgcag gtggtctaca cctgtgatga accagcctac agaaggctcg cccttccatg
     5401 ttccccaatg tgcggaagaa actggaacca gtccgtcgca atcagctcca taaccgttga
     5461 gcagaccaac agcagagcag attggccatg taagagccat cagtggatgc tggattggat
     5521 caagcagata catgtgtccg ctgtaagagt agtattttac tccggaagcg tttggtgtgg
     5581 cagcattgaa tgcagcgacg cctgactttg taagagagct gagagctgcc agagcgtttg
     5641 cgctg
//
LOCUS       DAOQJL010000215         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8255_length_5644_cov_4.319020, whole genome shotgun sequence.
ACCESSION   DAOQJL010000215 DAOQJL010000000
VERSION     DAOQJL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_5644_cov_4.319020"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLG78_10425"
     CDS             <1..405
                     /locus_tag="PLG78_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23576.1"
                     /translation="GDTRPKVLALLNKIARENSHLGMSPGEQFLDLVHVDDVCAAFET
                     AAVRMLKDQVQGHEVYAVSSSNPVQLREIVNIFNHAAGSKLDIGWGERPYRAREVMHP
                     YNKGIPVPGWEPRISLHEGIRDFIHSNQESKT"
     gene            402..1361
                     /locus_tag="PLG78_10430"
     CDS             402..1361
                     /locus_tag="PLG78_10430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE23577.1"
                     /translation="MKKKLISIVVPVYFEEELIAEFYSRMHPVMNQLKSRFDTELIFI
                     NDGSKDRSLEILLELTKKDSRVRILDFSRNFGHQRAITAGIDYADGDAVVIIDSDLQD
                     PPEVIPEMIAKWEEGYDVVGGTRKARKGETAFKLFTARIFYRLLNSLSEIPLPLDTGD
                     FRLMDRKVVKVIRTMKEEGRYVRGLIVWAGFRQSSVLYERDARFAGETKYSLKRMLGL
                     ALDAITSFSEKPLMLAVQLGLFITAGSFLAMLYLLISRILRPESVVAGWTSLFVAILF
                     VGGVQILVVGILGVYIGRIYRESRDRPLYIVQETYGFKKRNSK"
     gene            1358..2185
                     /locus_tag="PLG78_10435"
     CDS             1358..2185
                     /locus_tag="PLG78_10435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE23578.1"
                     /translation="MKSPEISVCIPVYNTEPFIAEAIESALNQTFKDFELIILDNRST
                     DNTLKIVRKFKDKRIRVVAHEKNLGAEANWNAALDQAKGKYIKILCADDYIYPDCLEK
                     QVEALRAESSAVLVCAARHIVDEKGKRIMKRGPRKSETVPGIEAVRRAIRGGTNIYGE
                     TSSALFRRDAQKKAGAFDASIPYVIDLDMWTRLLLHGNLILMDEAVSAYRVGAASWSA
                     EIMTKQKENFMRFIERIEANPAFCLTGTDAIIGRFRATLQAALRQLFYKFVLGWRKS"
     gene            2182..2598
                     /locus_tag="PLG78_10440"
     CDS             2182..2598
                     /locus_tag="PLG78_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE23579.1"
                     /translation="MNEEVHQRGLISHWLHLFMRAQFLRFLIVGGINTAFAYGVFSLG
                     LFIGLHYAVASLTSIILGMFFSFFTQGKLVFKSNKPEELLRFLGVATVMYLVHTGLLK
                     VASTFEIDLYLAGAVLTVPLALLSYVLNKHLVFRRN"
     gene            complement(2599..4596)
                     /locus_tag="PLG78_10445"
     CDS             complement(2599..4596)
                     /locus_tag="PLG78_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23580.1"
                     /translation="MTWITDANNRIHLHVKVMLAVVYGLTLYPMLEIGFTMQDDALLA
                     LAYQSKSLSPEPISYLIHSAVKESQYFGRVWVIYGSLMSYLPFAFHSAVYYKIVAFTS
                     LTLNVVLFFFVLQRLFRTLHFALLAVVLFLAFLQNSLHYNLITSFVAHFMTGISFLFA
                     SVLLFDRYLNTKKTWHHAGAGLCLFMSFFTYELFVLYYCLIPVLVLSSVMERHSLTRF
                     KEALPHWREIVRELVPTAAVLLVYLIIYAGYRLAYPTKYTGIEIAFSPARYIESVYRL
                     TIGALPISLYYRHSYDSVFAYLSENLQGHRSSIFYVLVRAHADWLARAVLLAFLALVV
                     LRDRPKRMSGRNLLWLCGFCLFLFWIPGTLPSLTSRYQIFVQMGLPMYLVTSFSYYAF
                     IVALTAVLIFVNERINHSALRRALHAIVVVIVFSWSILTDYTNFYVAKLQSQNQRQWL
                     IMDDFMHTPEFAAVPAGSIVYAPTLWQHSPLAPMVEVKEYWQDYIRAMTLKKLSIIKT
                     ESEFKRAFQAGELAGVDVYYLKFSQERKDPNQFLVFAKVNSSGVQNGRPFFRADHAAV
                     FMRSAYREYTAYVPLSGIEKTERVIVDGEERMLRGSFVAAPLDKSSVKGDLIKTEFRA
                     PAIDIERISVSNYVEQYEFPYNEKPNVEYDLEQERRKLLHQ"
     gene            4693..>5644
                     /locus_tag="PLG78_10450"
     CDS             4693..>5644
                     /locus_tag="PLG78_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNE23581.1"
                     /translation="MTLPGGKPQRQIVETFFATAARGTEECVEREIRDLAARYAESKI
                     KDIRRTRAGVEFKGTLQAGYLACLWLRTASRVLLKLSEFDAYSPEELYDGVADIDWSQ
                     HLKANQSLAVDCGISRSNMTHSHYGALKTKDAIVDQFMDHDGIRPGVDLKNPDVRINV
                     YVLEDYATLSIDLSGALHERGYRTEMTAAPLKENLAAALLLTAGFRESFEAGGTLVDP
                     MCGSGTLPIEALGIALDVAPGLDRKNFGFLRWKGHDSSLWASVFEDAKKRAAAGFDRA
                     YGERKGQFPFIGFDEDANAIRIASDTMLRMGFEGLVHFERR"
BASE COUNT         1543 a         1373 c         1433 g         1295 t
ORIGIN      
        1 ggagacacgc gacccaaagt gcttgctttg ctgaataaga tcgcgcgcga aaattcacat
       61 ctgggaatgt caccaggaga gcagtttctg gacctcgtgc atgtagacga tgtgtgcgcc
      121 gcatttgaaa cagccgcagt tcgcatgctc aaagatcagg tccaggggca tgaggtctat
      181 gccgtctcgt cgagcaatcc ggtccagcta cgcgaaatag taaatatctt taaccatgcc
      241 gctggcagca aactggacat tggctggggc gaaaggccat accgggcgcg cgaagtaatg
      301 catccataca acaagggcat acctgtgccc ggttgggagc cccgcatctc acttcacgaa
      361 ggcattcggg atttcatcca ttcaaatcag gagagcaaaa catgaaaaag aaactcatct
      421 ccattgtagt ccctgtgtac tttgaagagg aactgatcgc tgaattctat tccagaatgc
      481 atccagtgat gaatcaactg aagtccagat ttgacactga actgatattc atcaatgacg
      541 ggagcaaaga cagatctctg gaaatcttgc ttgagctgac gaaaaaagat tcgcgagtcc
      601 ggattctgga tttctcgcgc aattttggcc accagcgcgc catcacggcc ggcattgatt
      661 acgcggacgg ggacgcagtg gtcatcatcg atagcgacct gcaggatcca ccggaagtca
      721 tcccggagat gattgcgaaa tgggaagaag gctatgacgt ggttggcggc acgcgcaaag
      781 ctcgaaaagg ggaaacagcc ttcaaactct tcactgcccg cattttctac agactcctca
      841 acagtctcag tgaaatacct ctcccgctgg atacgggtga ctttcgttta atggatcgta
      901 aggtggtgaa agtcattcgg accatgaaag aggaagggag atatgtgcgt ggcctgatcg
      961 tatgggccgg atttcgtcag agcagtgtgc tctacgaacg cgatgcacga tttgccggag
     1021 aaaccaaata cagcctcaag cggatgctcg gactcgcgct ggatgcaatt acaagttttt
     1081 cagagaaacc tttgatgctc gcagtgcagc tgggtctttt catcacagct ggctcatttc
     1141 tggctatgct ctatctgctt atcagcagaa ttctcagacc tgagtctgtg gtcgcgggct
     1201 ggacttcatt gtttgtggcg attctgttcg taggtggagt gcaaatcctg gtggtcggaa
     1261 tccttggtgt ttatattgga agaatctatc gcgaatcgcg cgacaggcca ctctacattg
     1321 ttcaagaaac ctacggcttc aaaaaacgga attcaaagtg aaatcaccgg aaatctctgt
     1381 ctgcattccg gtgtataaca cggaaccttt cattgcagag gcaatcgaat cggccctgaa
     1441 tcaaaccttc aaagacttcg aactcatcat tctggacaat cggtctacgg acaatactct
     1501 gaaaatagtg cgcaaattca aagataagag aattcgcgtt gtggcgcacg aaaagaatct
     1561 tggcgcggaa gcaaactgga acgccgctct cgatcaggcg aaggggaaat acattaagat
     1621 cctttgtgca gatgattata tttaccccga ctgcctggaa aagcaggtag aagcccttcg
     1681 tgccgagtcg tctgcggtac ttgtctgtgc tgcccgtcac atcgtggatg aaaaaggaaa
     1741 gcgaatcatg aaacgtggac cccggaaaag cgaaacagtg ccgggcatag aagcggttcg
     1801 acgcgcgatc agaggcggta cgaacatcta tggggaaacc agttctgcgc tgtttcgccg
     1861 cgacgcacag aagaaagcgg gggcattcga tgcgtccatt ccgtatgtga ttgaccttga
     1921 tatgtggaca cggctgcttc tacacggaaa tttgattctg atggatgagg cagtctccgc
     1981 ttacagagtg ggggcggcat cctggagcgc tgaaattatg accaagcaaa aagaaaactt
     2041 catgcgcttc atcgagcgaa tcgaagcgaa tcccgccttc tgcctgaccg gaacggatgc
     2101 gatcattggc cgctttcgcg caacccttca ggcagcactc agacaactct tctacaaatt
     2161 cgttctggga tggagaaaat cttgaacgaa gaagtgcatc agcgcggcct gatttcgcac
     2221 tggctgcacc tgttcatgcg tgcacaattt ttgcgcttcc tcattgtggg cggaatcaat
     2281 accgcgttcg cctacggtgt gttctctctg gggttattca ttggcctgca ctatgctgtg
     2341 gcttcactaa catccatcat tctggggatg ttcttttcct ttttcactca gggaaaactg
     2401 gtcttcaaaa gcaacaaacc cgaggagctc ctcaggtttc tcggagtggc cacagtgatg
     2461 tacctggtcc ataccggact cctgaaggtt gcatctacat ttgaaatcga tctctacctg
     2521 gcgggcgctg ttttgactgt tccgcttgct ctattgtctt acgtcctcaa caaacacctg
     2581 gtgttcagaa gaaactgatc actgatgcaa caacttgcgt ctttcctgtt caaggtcgta
     2641 ctctacattt ggcttttcat tgtacggaaa ctcatattgc tccacgtaat tcgaaacgct
     2701 aatcctttct atgtcgatgg ccggggcacg aaattcggtt ttgatcaagt ctccttttac
     2761 agatgactta tccaggggcg cggcaacaaa cgaaccgcgt aacatccgtt cctctccatc
     2821 tacaatcact cgctccgttt tctcaatgcc agataacggt acgtacgcag tgtattcacg
     2881 gtatgcagat ctcataaaga ctgcagcatg atccgcgcga aagaatggac gtccattctg
     2941 aacgccagaa gaattcactt ttgcgaaaac caggaactga ttgggatctt tgcgctcctg
     3001 ggaaaacttt aagtagtaaa catctacccc ggcaagttcg ccggcctgga acgctctttt
     3061 gaattcactt tcagttttaa ttatggaaag cttctttagt gtcatggcac ggatataatc
     3121 ctgccagtac tcttttactt ccaccatagg ggccagcgga gaatgttgcc agagtgtcgg
     3181 agcatagaca atactacctg ccggcactgc cgcaaactcc ggagtgtgca tgaaatcatc
     3241 catgatcagc cattgtctct gattctgact ctgcaatttc gcaacgtaga agttggtata
     3301 gtccgtcagt atgctccacg agaatacgat gacgaccaca atggcatgaa gcgcacgtcg
     3361 caaagcggag tgattgatcc tttcgttcac gaatatcaaa accgcagtta gagccacaat
     3421 gaacgcgtaa taggagaaag aagtgaccag atacatggga agacccattt gcacaaatat
     3481 ctgatacctg gaagtcaacg aaggcagagt tcctggaatc cagaacagga acagacagaa
     3541 cccgcacaac cacagaagat tccgtcccga cattctttta ggacgatcgc gcagaactac
     3601 gagagctaaa aacgcaagca acacagcgcg cgcgagccag tctgcatgcg cacgaaccag
     3661 cacgtagaaa atgctgcttc tatgtccctg caggttttcg gaaaggtatg cgaatacaga
     3721 atcgtacgag tgcctgtagt agagggaaat cggcaacgct ccaatagtta acctgtagac
     3781 gctttcgatg tatcgagcag gtgaaaaagc aatttcaatg ccagtgtatt tcgtaggata
     3841 ggccaaccgg tagccagcat aaatgatcaa atatacgagc aatacggctg cagtcggcac
     3901 cagttcccga acgatctcgc gccagtgcgg caacgcttcc ttaaaacgag tcagtgaatg
     3961 ccgttccatt acgctactca ggaccagaac cggaatcaaa cagtagtaaa gtacaaacaa
     4021 ttcataggta aagaaagaca tgaacaggca caatcctgct ccggcatgat gccaggtctt
     4081 tttcgtgttc agatagcgat caaacaacaa aacggatgca aatagaaagc tgatcccggt
     4141 catgaagtgc gccacaaagg acgtaatcag attgtaatgc aggctattct gcaaaaacgc
     4201 gagaaacagc accactgcga gcagcgcaaa atgcaacgta cgaaaaagtc tctggagcac
     4261 aaagaagaac aaaactacgt tgagggtcag ggatgtgaac gctacgatct tgtagtacac
     4321 tgcgctatga aaggcaaacg gcaagtagct catgagagag ccgtagatga cccagacacg
     4381 cccgaaatac tgagactcct ttacggccga atgaatgaga taggagatag gctcggggga
     4441 aagcgacttg gactgatacg caagagccag aagggcgtca tcctgcatcg taaatccgat
     4501 ttctaacatt ggatagagag tcaggccgta aacgactgcc agcatcactt tgacgtgaag
     4561 atgtatccga ttgtttgcat ccgtaatcca ggtcatgcag cagggactcc aaactcaggc
     4621 aggcacgata ggtgccgcac gcaatcaaat ttttgttgcc tgggacgcgc ttcaaggcgc
     4681 gattcactat ccatgaccct gcccggcggc aagccccagc gccaaatagt ggaaacattc
     4741 ttcgcgactg cggccagggg aacagaagaa tgtgtggagc gcgaaatcag agacctcgcg
     4801 gcccgttatg cggaatctaa aatcaaagac attcgccgca ccagagctgg agtagaattc
     4861 aaaggcacac ttcaggcagg gtatcttgca tgcctgtggc tgcgaactgc gtccagagtc
     4921 ttgctcaagc tttcagaatt cgacgcctat tcaccggaag aactgtatga cggagtggct
     4981 gacattgact ggagccagca tctcaaagcg aaccagagcc tggcggtgga ttgtggtatt
     5041 tcgcgctcca acatgacgca ctctcattac ggggcactca agacgaaaga cgcaattgtt
     5101 gatcaattca tggatcacga tggaatcagg ccgggtgtgg atttgaagaa tcccgatgtc
     5161 cgcatcaacg tctacgttct agaagactat gccacgttga gtatagattt aagcggcgct
     5221 cttcatgaga gaggatatcg cacagaaatg actgccgccc cactaaagga gaatcttgcc
     5281 gcggctctgc ttctgactgc cggattcaga gaatcattcg aggcaggcgg gacacttgtg
     5341 gatcccatgt gcggctctgg gactcttccc atcgaagcac tgggcatagc actcgatgtg
     5401 gcgccaggtc tggatagaaa aaactttgga ttcctgagat ggaaaggtca tgactcttct
     5461 ctatgggcga gcgtctttga ggacgcaaaa aaaagagcag ctgcgggatt tgatcgagcc
     5521 tatggcgaac gcaaaggaca atttcctttc atcggatttg acgaagacgc aaacgcaatt
     5581 cgcattgcgt ccgatacaat gcttcgcatg ggctttgaag gactggtcca ttttgaaagg
     5641 agag
//
LOCUS       DAOQJL010000216         5629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8281_length_5629_cov_4.351094, whole genome shotgun sequence.
ACCESSION   DAOQJL010000216 DAOQJL010000000
VERSION     DAOQJL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8281_length_5629_cov_4.351094"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLG78_10455"
     CDS             complement(<1..428)
                     /locus_tag="PLG78_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNE23582.1"
                     /translation="METISFKAPEYQSDGSFQEIEYRFEGDEKSGWTIFRNGQKQMEL
                     GEGYTAVKSLYCGVCSTDLARRFLPYPLPQIIGHEVVGIKDNKPVVIEINASPLARHI
                     ETKDPFSLHGLHTHSPERITLGIDRLPGGFAPWILAPVDA"
     gene            complement(430..711)
                     /locus_tag="PLG78_10460"
     CDS             complement(430..711)
                     /locus_tag="PLG78_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23583.1"
                     /translation="MEWEKILRDSVKENAIRELHLRHIPVLKTCENWNDVKEIGLVDH
                     RTKYAHYKGLLVRYGERIYYVPEARMEALAPFRNWTSKVHIKVTDPEKK"
     gene            complement(730..2226)
                     /gene="gcvPB"
                     /locus_tag="PLG78_10465"
     CDS             complement(730..2226)
                     /gene="gcvPB"
                     /locus_tag="PLG78_10465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNE23584.1"
                     /translation="MQNQLSTTRNGQIFEEPLIFERSRPGRIGVSFPADDASLPIEID
                     SSLLRSAEDIHLPEVSEPEVVRHFTRLSTWNYGIDLNLYPLGSCTMKYNPRINEEIAA
                     SAQFADLHPNTPEKYAQAGLRAVYELQEVLKTVTDMPGITLQPAAGAHGELTGLFLVK
                     AYLKKKGDTHKTVVLVPDSAHGTNPASCTLAGFTVRELPSTAEGLLDVEEFKKALTPD
                     VAALMITNPNTLGIFETNIPLVADLLHKNGSLLYMDGANYNAIVGKVSIGKMGVDISH
                     LNLHKTFSTPHGGGGPGAAAVVVSDRVREFLPGPIVEKKESSQGPVYVVTSPSASIGR
                     VKAGPGHFGMIIRALTYILSYGNELHRVAEQAVLNANLVRKSLQEVFEPASGLDTMHE
                     AVFSDAKQRKTGLETIHLAKTLIDYGYHPPTIYFPLSVHGAIMIEPTETESPETIAHF
                     CAVMKDLAARMNGQDESMKSSPTRSFVRRIDEVEAARKPVLNYFTKKA"
     gene            complement(2241..3566)
                     /gene="gcvPA"
                     /locus_tag="PLG78_10470"
     CDS             complement(2241..3566)
                     /gene="gcvPA"
                     /locus_tag="PLG78_10470"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNE23585.1"
                     /translation="MNPHKYLPVSEKEYQEELSSLSLKSADDLFRCIPEALRGNTTTS
                     LRSPMSEMEIREHFRPGVEKIQHTFIGGNGHTHYIPSIIGPLISRGEFLTAYTPYQPE
                     ISQGTLQAMFEYQSMMATLMGVEISNASLYDGSTAMLEACYMAQRITGKSSILFSEQI
                     HPEYIETMRTYSEGGIFQFDFVSADASGRIDVQKLSGKISDNTAALVVQTPNFSGIIE
                     DLTALKKILEAKGILLIAVITEAMSLALVKSPGACGADIVCGEAQSFGVPLSYGGPWL
                     GFLGASPAFMRNMPGRLIGEAKDANGERAFVVTLATREQHIRREKATSNICTNQGLMA
                     LRAAIYLSVMGKHGIRRAAERCARFAAYGRKKASSIAGVQVIFSSSPIFNEFVIETKK
                     DANEVFEHCVEKFGIAPGSVIGKNRLHVSFHEMHSKEIVDRWAEALASA"
     gene            complement(3586..3981)
                     /gene="gcvH"
                     /locus_tag="PLG78_10475"
     CDS             complement(3586..3981)
                     /gene="gcvH"
                     /locus_tag="PLG78_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNE23586.1"
                     /translation="MDSKIPEGYLFTKQHEWAREEGGFLTVGISDYAQNALGDIVYVD
                     LKAPGTKVEAGKTFGTIESVKAAEDLYTPVSGVIDSLNPAVKDKPEAVNKDAYGTWLI
                     KLKDFDKGALSGLMNSADYAKYLASLDGH"
     gene            complement(4011..5111)
                     /gene="gcvT"
                     /locus_tag="PLG78_10480"
     CDS             complement(4011..5111)
                     /gene="gcvT"
                     /locus_tag="PLG78_10480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNE23587.1"
                     /translation="MNLKKTPLNSIHHSSGGRMVPFAGWEMPVQYSSILQEHQAVREK
                     AGLFDVSHMGEIEVKGTTAEEFLDSVTCNRVQEMQDGQVQYNAVLNEKGGIVDDITIY
                     RVNPESFFVVSNASNYEAVTAHFEKYKIAGTDVQNISNTIHQIAIQGPLAEDILRDFL
                     NLDLSSIGYYRFKDMDSRFGKLRLSRTGYTGEDGFEIYSDVNAGVEIWKGILAQKSSS
                     GLIACGLGARDLLRLEARYPLYGHELNETWTPVESGIGWIVKEKTSVMPGYARVLEQK
                     KSGPANQVVGFKLMEGGVPREGYAVVNESGAELGKVLSGGFSPIVNAGIGTAFLPVGL
                     EAFYIQIRDKKVPAKAEKKAFVQGTAGKNRQK"
BASE COUNT         1285 a         1342 c         1399 g         1603 t
ORIGIN      
        1 attgcatcca ccggtgcaag aatccacgga gcaaatcctc ctggaagtct gtcaattccc
       61 agagtgatcc tttcaggcga atgagtgtga agaccgtgga gagagaatgg atcttttgtt
      121 tcaatgtgac gcgcgagtgg agatgcattg atttcaatga cgactggttt gttgtctttg
      181 atgccaacta cttcatgtcc gatgatctga ggcagaggat acggcagaaa tcttcgcgca
      241 agatccgtag agcacacgcc gcagtagaga gattttacag cagtgtagcc ctcaccgagt
      301 tccatctgct tctggccgtt tctgaaaatg gtccagcctg acttttcgtc tccctcgaag
      361 cggtattcaa tctcttgaaa gctgccgtcg ctttgatact cgggagcttt gaaagagatt
      421 gtttccaagt tatttcttct ctgggtcggt tactttgata tgaaccttgg atgtccagtt
      481 gcgaaagggt gctaaagctt ccatacgagc ttccgggacg tagtagatgc gttcgccgta
      541 gcgaacgaga aggcccttgt agtgagcata tttggttctg tgatcgacca ggccgatctc
      601 ttttacgtcg ttccagttct cacaggtctt gagaactgga atatgccgga ggtggagctc
      661 ccggattgcg ttttccttta cggagtccct gaggatcttc tcccattcca tccattagtt
      721 tccaacagat caggcttttt ttgtaaagta atttagtacc ggctttcgag cggcttccac
      781 ttcgtctatg cgacgaacaa agctccgcgt cgggctggac ttcatacttt cgtcctggcc
      841 attcattctg gccgccaggt ccttcatcac agcgcagaaa tgcgcgattg tttccggaga
      901 ctctgtctca gttggctcga tcatgatcgc cccatggacg ctgagcggga aatagatggt
      961 aggtggatga tatccgtagt ctatgagagt ttttgcaaga tgaatggttt cgaggcctgt
     1021 tttacgctgt tttgcgtcgc tgaagaccgc ttcatgcatc gtgtccaggc ctgaggctgg
     1081 ctcgaagact tcttgcagag actttcgcac aagattcgcg ttgagcactg cctgctcggc
     1141 gacgcggtgc agttcattgc cataagacag aatataagtc agagcacgaa tgatcattcc
     1201 aaagtgaccg gggccagctt tgactcttcc aatggacgcg gaaggcgatg tgacaacata
     1261 gaccgggccc tgtgagcttt cttttttttc cacaatgggt ccaggtagaa attcgcgcac
     1321 cctgtcagat acaacgactg ccgccgcacc tggtccgcca cctccatgag gagtgctgaa
     1381 tgttttgtgg agattcagat ggctgatgtc cacgcccatt ttgccaatag acaccttgcc
     1441 tacgatggcg ttgtagttgg ctccgtccat atagagaaga gaaccattct tgtgtaaaag
     1501 atctgccacc agaggaatgt ttgtttcgaa gatgcccaga gtgttcgggt tcgtgatcat
     1561 gagagcagcc acatctggag tgagcgcttt cttgaattcc tctacatcca gaaggccttc
     1621 cgcggtggag ggcagttcac ggacagtgaa gcctgccaga gtgcaggaag caggattggt
     1681 tccatgtgcg gaatccggga caagcaccac tgttttgtgc gtatcaccct tcttttttaa
     1741 gtatgctttt acaaggaaca gcccggtcag ctcaccgtgg gcacctgccg caggctgcag
     1801 cgtgatcccg ggcatgtctg tgactgtctt gagcacctct tgcagttcgt agactgcgcg
     1861 aaggcccgcc tgtgcgtatt tttcaggagt gtttggatga aggtccgcga actgagcgct
     1921 cgctgcaatt tcctcgttga tgcgaggatt gtatttcatc gtgcagcttc ccagaggata
     1981 gaggttcaaa tcgattccgt agttccaggt ggagagtctt gtgaagtgtc gcaccacttc
     2041 cggttcagaa acttccggaa ggtggatgtc ctctgcgctg cgaagaagag atgaatcgat
     2101 ttcaattgga agagatgcgt catccgcagg gaagctcacg ccgattcttc cagggcgaga
     2161 tctttcaaaa ataaggggtt cttcgaagat ctgcccgtta cgagtggtgc tcagttgatt
     2221 ctgcattgct tttctccggc ttacgccgat gccagggcct ctgcccagcg atctacgatt
     2281 tctttcgaat gcatttcgtg gaacgaaacg tggagtctgt tcttgccaat gacgcttcca
     2341 ggtgcgatgc cgaatttttc cacgcaatgc tcgaagactt cgtttgcgtc tttctttgtc
     2401 tcgattacaa attcattgaa aatgggagag gaactgaaga ttacctgcac acctgcaatc
     2461 gaggacgctt tctttctgcc gtaggctgcg aatctggcgc atctttctgc ggcgcgacga
     2521 attccgtgtt tgcccatcac agatagatag atggccgcgc gaagggccat gagaccctgg
     2581 tttgtacaga tattggaagt tgctttctcg cggcgaatgt gttgttctcg cgtggcgaga
     2641 gtgactacga atgcgcgttc tccgtttgca tcttttgctt ctccaatcaa tcttccaggc
     2701 atatttcgca taaaagcggg agatgctccg agaaatccga gccatggtcc gccgtaggag
     2761 agtggaactc caaagctctg tgcttctcca catacaatgt ctgcgccgca tgcgcctgga
     2821 gatttcacca gtgcaagaga cattgcttcc gtgatcactg cgatcaacag tatacctttt
     2881 gcttctagaa ttttcttcag tgcagtgagg tcttctatga tgccggaaaa gttgggcgtt
     2941 tgcacaacga gtgctgctgt gttgtcagaa attttccctg aaagtttttg aacgtcgatt
     3001 ctgccggatg catctgcaga aacaaaatcg aactgaaaaa ttccgccctc tgaatacgtg
     3061 cgcatggttt cgatgtattc tggatgaatt tgttctgaga agaggatgga tgatttcccc
     3121 gtgattctct gtgccatgta gcatgcttca agcatcgcag tggagccatc gtagagagac
     3181 gcattcgaaa tttccacacc catgagtgtg gccatcatgc tctggtattc gaacatggcc
     3241 tgcagtgtgc cctggctgat ttctggctgg tagggtgtgt atgctgtgag gaattctccg
     3301 cgcgagatga gcggaccaat gatggaggga atgtagtgag tgtgtccgtt tcctccaatg
     3361 aatgtgtgtt ggattttttc tacgcctgga cgaaagtgct cgcggatctc catctctgac
     3421 atgggcgaac gcaacgaagt ggttgtattt ccgcgaagag cttccggaat gcagcgaaac
     3481 aaatcgtctg cgctcttcaa agagagagac gagagctctt cctggtattc cttttcagat
     3541 actgggagat acttgtgtgg gttcatttgc tactgccttt agaaatcaat gcccgtcgag
     3601 gctggccaga tacttcgcat agtctgccga attcatcagg ccggaaagcg cgcctttgtc
     3661 aaagtccttg agtttgatca gccaggttcc gtatgcgtct ttgttgaccg cctccggttt
     3721 gtctttcacg gcggggttca gggaatctat gacgccagag acaggagtat agagatcttc
     3781 tgctgctttt acggattcga tggtgccgaa agtttttcct gcttctacct tcgtgccggg
     3841 agccttcaaa tctacgtata caatgtctcc cagggcgttc tgggcgtagt cagagatccc
     3901 aacggtcaaa aagccgcctt cttctctggc ccattcgtgt tgttttgtaa aaagatagcc
     3961 ttctggtatt tttgagtcca tctagattct cctgattgcc cggctgaggg tcacttctgc
     4021 cgatttttcc cggccgtccc ctgaacaaac gcttttttct ctgctttcgc aggaactttt
     4081 ttgtcgcgaa tctgtatata gaaagcttcg agccctacag gcagaaaggc agttccaatc
     4141 cccgcgttta caataggaga aaagccacca gaaagaactt tgcccagttc cgcgcccgac
     4201 tcattcacaa ctgcatatcc ttcgcgcgga acgcctcctt ccatgagttt gaagcccact
     4261 acttgattgg ccgggccact cttcttttgc tccagcacgc gtgcataacc aggcattacg
     4321 gaggttttct cttttacaat ccaaccgatt ccactttcta caggtgtcca tgtttcattg
     4381 agttcgtggc cgtagagagg atagcgcgct tcgagtcgaa gcaaatcgcg tgcaccgagt
     4441 ccacatgcaa tcaacccgct gcttgatttt tgtgcaagaa tgcctttcca gatttccact
     4501 cctgcgttca catcggaata gatttcaaat ccgtcttctc cagtgtaacc ggtgcgactc
     4561 agtctgagtt ttccaaatct tgagtccata tctttgaagc gatagtaacc aatggaagaa
     4621 agatctagat tcaagaaatc gcgaagaata tcttctgcca gtggtccctg gattgcaatt
     4681 tgatgaatgg tgttgcttat gttctgaaca tctgtgccgg cgattttata tttctcaaaa
     4741 tgtgctgtaa ctgcttcata gttagaagca tttgagacaa cgaagaaact ttctggattt
     4801 acgcgataaa tcgtgatatc atccacgatt ccgcccttct cattcaatac tgcattgtat
     4861 tgaacctgtc catcttgcat ttcctgtacc cgattgcagg tgacagaatc caggaattct
     4921 tcagcagttg tgccctttac ttcgatctct cccatgtgtg aaacatcaaa tagtcctgct
     4981 ttttcgcgta cagcctggtg ttcttgcagg atagaagagt actgaacagg catttcccat
     5041 ccagcaaacg gaaccattct gcctccagat gaatgatgaa ttgaattcag cggtgttttt
     5101 tttaggttca tttggaaaat tccgtggatt ggattttcca gaagtaaatt tgcacgctgc
     5161 ttctgctata aattcgcgca cgatttagcg aaaatttttt tttggtgcaa gttcttcata
     5221 attgttttga ccgtcggagc ccgtttgatt tcatcgcatc ttcttgaagc ggattgttta
     5281 tctcctcaga gagggtgtga attgtccgtg tataattttc tgtcacatta gttttgcgaa
     5341 attcgcggag agaatgtgag aacgcaatac tacgaagaac gattgttgaa tacatgaatg
     5401 agatgaagga aaagactgag caatcaatgc aaaagaagca gtcctgtgca caatgcgggg
     5461 aaacatctga taaattgtat cagcacaatc tctgcaaaga atgcttgaag cagagttttc
     5521 agcgtctgat tccaatgatc aacgaattcc gcatccgcaa ttgagttctg aacatccatt
     5581 gaagcgtcag attagctctg cccccaccaa tctctgcata cgtttcctc
//
LOCUS       DAOQJL010000217         5621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8304_length_5621_cov_5.299677, whole genome shotgun sequence.
ACCESSION   DAOQJL010000217 DAOQJL010000000
VERSION     DAOQJL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_5621_cov_5.299677"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLG78_10485"
     CDS             complement(<1..193)
                     /locus_tag="PLG78_10485"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNE23588.1"
                     /translation="MSLRKIDSALQMEEACCAFFAQFLESLPVSVVLLRGEPGAGKTT
                     FCRGFMRAMSIRENINSATF"
     gene            complement(205..399)
                     /locus_tag="PLG78_10490"
     CDS             complement(205..399)
                     /locus_tag="PLG78_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23589.1"
                     /translation="MARDIMERFQPELVQIPSVITGIFLLKWIGIFAGVVLVSFWGAG
                     AVVYKNLSGRDLSDRDDWRR"
     gene            637..1761
                     /locus_tag="PLG78_10495"
     CDS             637..1761
                     /locus_tag="PLG78_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23590.1"
                     /translation="MKKYLGIGLILAVSSMAPLRAQIVCTGPGCQYLPLSQATLDQMY
                     YDFKTQYTDQLFKDMAAAGVLANLTGAPTGTVNLYGWTIGASAGLGYIPIKEVSIPIT
                     GYGELKDIPRAGGAVNPRIFFGANVGWLLNQAYDPWGDGESSEPFFLSLSRFDLYGSF
                     IQDRRHYVMQDSGQVTVNTRNLGFDVRYHLVEGKPLAAGPLLRFLGVSVGAGYHTAAM
                     DITAIQNQNQKMKITLTGGSNLIWDGANQGKIQTQVKSMPLEIMTGLQILYALNMSFG
                     GGMSINRGSADVTLTRLGPVYLSNDSAALLAAATGQTVASSAYNSYLTMQVTGSGQAP
                     RNMAYAKLGLDFNILWFKIFAEGLWAKRAYGANLGVRWQM"
     gene            complement(1765..2529)
                     /locus_tag="PLG78_10500"
     CDS             complement(1765..2529)
                     /locus_tag="PLG78_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNE23591.1"
                     /translation="MTFVFKRLTLVAILLVVVSACRVAEPRGNSMGQDYLEREFKCTE
                     TPLPVEVDPGFGLIKQAVRQNWGNENRKSARIVLTGDSTIALFAGPRLQAYLPGLDVA
                     NRGIGGDTSGGLLARLDTDVVALHPSTVVIVIGGNDILQGRCMRTVLANTTSIFKTLK
                     ESLPGVRIIMVSIPPVVSWKANSITPYYNRKLEYLTRDEGIEYLDLWPALSDSDKPQL
                     KEEFYFRLPGGKVDQVHFNDKGYETFGRLLQPLLSR"
     gene            complement(2526..3815)
                     /locus_tag="PLG78_10505"
     CDS             complement(2526..3815)
                     /locus_tag="PLG78_10505"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE23592.1"
                     /translation="MFGPIKKRKDPLTILVSIIIILLFAFLAGTAALVMAAFLAVLVL
                     FYPFFLYWWDKLYGTRDIADQLFYAHTDDGWNIAMHFHMPRYPKANAYPVIMSHGIAV
                     NKYGVDLDEAHSLPYFLKQNGYPVFVLSLRGCGKSYHSSKGRYRDFNFDDIVMYDVPA
                     VIRRVKELTGAPKVNWVGHSMGSMIMYGFLGRELPGSEDIACFTALASPGKIDHAKTT
                     IWGAIANMPWLPRVLDMKFGAQIISPLTGRFLTPIDNIVYDSENISKVTIRRLMKNGI
                     ENIGAGLISQFLDWIKSGVEHTRDGSHSYRDGFKNIKAPALFLAGTRDHIAPAESVRF
                     AYEQCGSKIKEFRLLGKATGFHCDYCHTGIVLGDRARDDVYPLVRDFLDEYGMKKRRR
                     NFIKAIFKRFIRKRRQRQLKGLRIFREPAKPGSIIVS"
     gene            3861..4292
                     /locus_tag="PLG78_10510"
     CDS             3861..4292
                     /locus_tag="PLG78_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23593.1"
                     /translation="MQSESGGYQTFQGPFFSIRLPSHWEIEVIENMPAFFDPEGNGVL
                     QVAALKRQGQDYDIQAELERYLAQNQIEADPERMSRFRLPSGLNGIAVEYIVDNRFWM
                     VNALGAGNQCLLAIYNSDEVPDQGMAMLISEALASIEFMHS"
     gene            4289..4894
                     /gene="purN"
                     /locus_tag="PLG78_10515"
     CDS             4289..4894
                     /gene="purN"
                     /locus_tag="PLG78_10515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNE23594.1"
                     /translation="MKRIVVLASGRGSNFRAVLSAIEDHRIAGAHILALVSDRSGTGA
                     EQIARDANIPITLVDYSDKSKRASSDENLTKVISNLAPDCVLALGFMRILPEKLVLAF
                     RHKIINVHPSLLPAFPGMNAQKQALEYGVRVTGATVHFVDTGVDTGPIILQSAVQVPA
                     GATLEEITDLIQAEEHRIVVQALDLFLKDQIQVDGRKVLIK"
     gene            4908..>5621
                     /gene="purH"
                     /locus_tag="PLG78_10520"
     CDS             4908..>5621
                     /gene="purH"
                     /locus_tag="PLG78_10520"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNE23595.1"
                     /translation="MIQIKRALISVSDKTGLNDFARFLAGRGVDIISTGGTLKTLQEA
                     GIKARAVEEVTGFPEIMDGRVKTLHPKIHGGLLSLRDKQSHLDAMKAQQIEGIDLLIV
                     NLYPFAATIARDASFEDSIENIDIGGPAMLRAAAKNYRFCAAVTDPSDYSRLKEEIER
                     AGGIPEKMSLNLARRVFHHTAEYDSLISQYLDQIEAVKFPDSLTIAFRKVQPMRYGEN
                     PHQEAAYYRPLIDIERERRT"
BASE COUNT         1471 a         1444 c         1392 g         1314 t
ORIGIN      
        1 taaatgtagc gctattgata ttctcgcgaa tcgacatagc tcgcatgaat ccgcggcaaa
       61 aggtggtctt tccagcacca ggctctcctc gcaacaagac cacacttact ggaaggcttt
      121 cgaggaattg cgcgaagaaa gcgcagcagg cttcctccat ctgcagtgca gaatcaattt
      181 tccgtaaact catcaaagac tcggtcaacg acgccaatca tcgcggtcgc ttaaatcgcg
      241 gccggagaga ttcttataaa cgactgctcc cgctccccag aaggaaacca gcacaactcc
      301 cgcaaaaatc ccaatccact tcaggaggaa aataccagtg atcacagaag gaatctggac
      361 gagctcaggc tggaatcgct ccattatgtc gcgtgccaca cccgcaaata tgaaaaaaaa
      421 gacagagaag acaatcgtat agaggacctt tcgggcagct cgcatggatc agattcgtca
      481 gagaatcgac aaaaacaact caaaaccata gatcggggcc gccgaccaac cgatgtaccg
      541 tctgggggtt tgtttgacaa aaaatgaatc cgagtttgtt atcattatta tagcggagcc
      601 acggattggc ccgtctgatt gtaccgggga aggtgtatga aaaagtatct tgggatagga
      661 ctcattctgg ctgtgagcag catggctcct ctccgcgcgc aaatcgtttg tacgggaccg
      721 ggatgccagt acctccccct gagccaggca accctggatc agatgtatta cgattttaag
      781 acgcaataca cggaccagct cttcaaggac atggctgcgg caggagtgct tgcaaacctg
      841 actggagcac ccaccggaac ggtgaatctc tacggctgga ccattggagc cagcgcagga
      901 ctgggttaca tcccgataaa agaagtcagc attccaatca caggctacgg cgaactcaaa
      961 gacatcccgc gcgctggcgg cgcagtcaac cctcgtatct tttttggagc gaatgtaggc
     1021 tggctcctca accaggcgta tgatccgtgg ggtgatggag aatcctctga gccatttttc
     1081 ctctccctct ctcgattcga cttgtacggc tcattcatcc aggaccgaag gcactatgtg
     1141 atgcaagata gcgggcaagt tactgtaaac acccgtaatc tcggatttga tgtgcgatat
     1201 catcttgtag aaggcaaacc tctggcagcc ggtccacttc ttcgcttcct cggcgtgtct
     1261 gttggtgctg gttatcacac ggccgcgatg gacatcaccg ccatacagaa tcagaatcag
     1321 aaaatgaaaa tcaccctgac aggtggttcc aatttgatct gggacggcgc caatcagggg
     1381 aagatacaaa cccaggtaaa atccatgccg cttgaaatca tgactggtct gcaaattctc
     1441 tatgcgttga acatgtcgtt tggtggagga atgagcatca atcgtggatc agcagatgtg
     1501 acactgactc gactcggtcc tgtttatctg tctaacgact cagcagcact gcttgcggcc
     1561 gccaccgggc aaacagtcgc ctcttccgcg tataattcct atctgacaat gcaagtaacg
     1621 ggaagtgggc aggctccgcg taacatggcg tatgcgaaac tgggactgga cttcaacatt
     1681 ctctggttta agattttcgc agaaggtctg tgggctaaac gcgcttacgg tgcaaacctt
     1741 ggcgttcgct ggcaaatgta ggatctacct ggaaagaagc ggctggagaa ggcgtccgaa
     1801 agtctcgtag cccttgtcat tgaaatggac ctggtccacc tttccgcctg gaaggcgaaa
     1861 gtaaaattct tccttgagtt gcggcttgtc tgaatcagaa agagcaggcc ataaatccag
     1921 atactcaatc ccttcgtccc gcgtcaggta ctccagcttg cgattgtagt agggagtgat
     1981 gctgtttgct ttccacgata ctaccggtgg tatgctgacc atgatgatcc ttacgccggg
     2041 caatgattcc ttcaaggtct tgaaaatgct tgtggtgttt gcgagaaccg tacgcatgca
     2101 tctgccctgc agtatgtcat ttccgccaat cactatcaca accgtggaag gatgaagggc
     2161 caccacatcc gtatccaaac gcgcgagcaa tccacccgat gtatctccac caatgccgcg
     2221 gttagcgaca tccaatcctg gcagatacgc ttgcagacga ggtcccgcga aaagggcgat
     2281 ggtggaatca ccggtcaaaa caatgcgcgc actctttctg ttctcgttgc cccagttctg
     2341 cctgacagcc tgcttgatga gcccaaatcc aggatcgact tcaacgggca gaggcgtctc
     2401 tgtgcatttg aattcgcgct ccaggtaatc ctggcccata gaattgccac gaggctccgc
     2461 tactctgcag gcggagacaa cgaccagcag aatggcgact aacgttaagc gcttaaaaac
     2521 gaatgtcatg atacgatgat gctgcccggt tttgccggct ccctgaatat gcgaagtcct
     2581 ttgagttggc gctgcctgcg ctttcgaatg aatcttttga agatcgcctt gatgaaattt
     2641 ctgcgtctct ttttcatccc gtattcgtcc aggaagtcgc gcacaagagg atacacatca
     2701 tcgcgcgcac gatcccccag aacgattcct gtgtgacagt agtcgcaatg gaagccggtt
     2761 gcttttccaa gaagtctaaa ttccttgatc ttacttccgc attgttcgta agcaaacctg
     2821 acagattctg caggagcaat gtgatcacga gtaccggcca ggaagagtgc aggcgctttt
     2881 atgtttttaa atccgtctct gtaactgtgt gaaccatccc gagtgtgctc cactccactc
     2941 ttgatccagt cgaggaactg cgagatgagt cctgctccaa tattctcaat cccgttcttc
     3001 atcaatctgc gaatggttac tttcgaaata ttttctgaat catatacaat gttgtcaatg
     3061 ggagtcagaa acctgcctgt gagcggggag atgatctgtg caccaaactt catatcaagc
     3121 actcgaggaa gccacggcat gttcgcaatc gcaccccaga tggtcgtctt cgcatgatca
     3181 atcttcccgg gactggccag tgctgtgaaa caggcaatgt cttctgaacc aggaagttct
     3241 ctgcccagaa atccgtacat aatcatggat cccatgctat gacccaccca gtttactttg
     3301 ggtgcaccgg tcagttcttt gaccctgcga atcactgcag gcacatcata cataactatg
     3361 tcatcaaaat tgaaatcgcg gtatctgcct ttggaagaat gatacgactt tccgcatccg
     3421 cgcaaactca acacgaacac tggatatcca ttctgtttca gaaagtatgg aaggctgtgc
     3481 gcttcgtcca gatccactcc gtatttgttc accgcaatgc cgtgagacat gatcacagga
     3541 taggcgtttg ctttggggta gcgcggcatg tgaaaatgca tcgctatgtt ccagccatcg
     3601 tccgtgtgcg cgtaaaagag ctgatcggca atgtcccgcg tgccgtagag tttatcccac
     3661 cagtagagga agaacggata aaataggacc aggacggcca gaaacgccgc catgacgagc
     3721 gcagctgtgc ccgcgagaaa tgcaaaaagc aaaatgatga tgatcgagac caggatggtc
     3781 agcggatctt tgcgtttttt gatgggacca aacaaggtgc tttacaaaat gcgtcgagag
     3841 tgcccttgag caactaaaat atgcagagcg aaagcggcgg ttaccagacc tttcagggtc
     3901 cctttttctc cattcggctc ccatctcact gggaaatcga agtgatcgag aatatgcccg
     3961 cgttttttga cccggaaggc aatggagtgc tgcaggttgc ggccttgaaa agacagggtc
     4021 aggattatga tatccaggca gaactcgagc gatatctggc gcaaaatcaa attgaagcag
     4081 atccggagcg aatgtccagg tttcgcctgc catccggcct gaacggaatt gcagttgagt
     4141 acatcgtgga taaccgattc tggatggtca acgcgttggg cgcggggaat caatgcttgc
     4201 ttgcaattta taactcagat gaagtaccgg accagggcat ggccatgcta atatcggaag
     4261 ctcttgcttc catagagttc atgcattcat gaaacggatt gtggtacttg cttctggtcg
     4321 tggatccaac tttcgcgcag ttctttcggc catcgaggat catcgcatcg caggcgcgca
     4381 catcctcgct ctggtctctg atcgatctgg aacaggagca gaacagatcg cgcgcgatgc
     4441 gaacattccc atcactcttg tagattattc agacaaatcg aagcgcgcgt cctcggacga
     4501 aaatctaaca aaagtcatct cgaatctcgc gccggattgc gttcttgcac tcgggtttat
     4561 gcgaatcctc ccagaaaaat tggttcttgc ctttcgtcac aaaatcatca atgtacaccc
     4621 ttcccttctt cctgcttttc caggaatgaa tgcgcaaaag caggcgctcg aatacggcgt
     4681 tcgcgtaacg ggagcaacgg tgcattttgt ggacactgga gtggatacag gcccaatcat
     4741 tctacaatct gcggttcaag ttcccgcagg ggcaactctg gaagaaatca cggacctcat
     4801 tcaggcagag gaacatcgca tcgtagtgca ggctctggat ctttttctca aggatcaaat
     4861 ccaggtggac ggcagaaagg tactcataaa ataaccagga aaacagcatg attcagatca
     4921 aaagagcgct catctcagtt tcagacaaaa ccggcctaaa cgatttcgca agattcctcg
     4981 caggaagagg cgtagacatc atatcgacag gagggacact gaagactctg caggaagcag
     5041 ggatcaaagc gagggcggtg gaagaggtca ccggttttcc tgaaatcatg gacggaagag
     5101 taaaaactct tcacccaaag attcacggcg gccttctctc tttgcgagac aaacaatcgc
     5161 acctggacgc aatgaaagcg cagcaaatag aagggattga tcttctgatt gtgaatcttt
     5221 atccatttgc cgcgaccatc gcacgcgacg cgtcttttga agattccatt gaaaacatag
     5281 acattggtgg accggccatg ctgcgcgccg cagccaagaa ctatcgcttt tgcgcggcag
     5341 taacggaccc ttctgactat tccagactca aagaggaaat cgaaagagcg ggcggtattc
     5401 ctgagaagat gtccttgaat ctcgcgcgac gcgttttcca tcacacagca gaatatgatt
     5461 cgctgatttc tcagtacctc gatcagatcg aagcagtaaa atttccagac tcacttacaa
     5521 ttgcatttcg caaggtgcaa ccaatgcgct acggcgaaaa cccgcatcaa gaagctgcct
     5581 actatcgtcc tctgatcgac atagaacgcg aaagaagaac c
//
LOCUS       DAOQJL010000218         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8316_length_5616_cov_4.411437, whole genome shotgun sequence.
ACCESSION   DAOQJL010000218 DAOQJL010000000
VERSION     DAOQJL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8316_length_5616_cov_4.411437"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..698
                     /locus_tag="PLG78_10525"
     CDS             216..698
                     /locus_tag="PLG78_10525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23596.1"
                     /translation="MLLISLEQPGSAAKEGISEAIAYAALESPRLADVIASVHKVAFE
                     KGGILRAGVYELREDEAPGEVPGLFHAVTAGLPPPLLIRGGEFTFPLRSGIPAGIVSE
                     WVSQTQSMEWHAGDALYLYTDSAEVEASHDFHEYLRAHKGGSLQIPEDMIVFRIEFGS
                     "
     gene            743..1123
                     /locus_tag="PLG78_10530"
     CDS             743..1123
                     /locus_tag="PLG78_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23597.1"
                     /translation="MWMWPALIVSIVLGAIGQILMKIGMKAAGPVPLDRGIVDLFYYF
                     MHAVLSLPLIGAALSYGVSFVLWLGVLSVADLSLARPIMSAGYILTLAYGFASGEDIT
                     PARVLGTCLIVVGIAFVAKSGIRT"
     gene            1120..2163
                     /locus_tag="PLG78_10535"
     CDS             1120..2163
                     /locus_tag="PLG78_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE23598.1"
                     /translation="MKAAVLEKDARFLQIKEVERPALAAGMVRVKVHACGVCGSDVHL
                     VLHRSMHAKSYPRIPGHESSGVVIEVGENVTRVKPQDRVVVSAGTSCGHCAACKAGRE
                     NLCPEVGVLGFDSDGSFAEEVVVPERCLFVFSSHIPFEQAAILADAVSTPYHALRYAG
                     NIKQGDTVAVFGCGGLGIHGVLLARALGASRVFALDVEAGALSNAEKAGANEIIDLSG
                     KSSVGKMNAGKILKEKGGVDLVVDFSGYYSNIEESVRAMNPGGRMVMVGLGRNPLKFA
                     IPATLIFKMISVCGSYGSDSRALPELIDLLEKGKINLAPSITSTHPLEEAQECIEKLE
                     NRKGNPIRFVIRP"
     gene            2166..3368
                     /locus_tag="PLG78_10540"
     CDS             2166..3368
                     /locus_tag="PLG78_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23599.1"
                     /translation="MAALLTLALVWFVPFDASLVKAALVALALLDSFFPVTSRSRMII
                     VSWIVLSQLILAFTGFYFSDDSLRHLHDGFYLLRGIDIYLFPPAALPVVVPTPDYHRM
                     IGSVYFPVVQFMSMIGSLFSVSRGYVFVFHGLCSLLFGILAMRIRGRVLSFFSRFVFS
                     PAFVLIASGRHEDLLAAAIFFLSLTLHSRGAGFFTGLLPFIKPDAAFLSLLTMRKNAA
                     YLLAMIVSASCLLWFSFQFLVISDLTLPAFLSNLRFFADWYVGYNPLSLPGPLFFVRI
                     RPWIALAAFLAGLVFIWRAASSGPSRRFVSILAATGAAGVLFSFALRGVWHPWYFCSA
                     ISALIFFKKRKLAQSIPPYLVLFFLPIADLRAGGGFHFGGFYVAVLGLLVLHLGHKFL
                     TRRNSDRL"
     gene            3373..4242
                     /locus_tag="PLG78_10545"
     CDS             3373..4242
                     /locus_tag="PLG78_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE23600.1"
                     /translation="MSREIESLLLSLYDSSDEKESAYLTKELFRTIPIGYHPLRYVEA
                     LSTPAVRWKLRTGLFPALGDLKIRHDLKELLNRSREDGDMDLEMGAFLIARGVEEPEV
                     VPEDLTVQLDALAKPLARAIKYAGGDPESRLNLFRFYMFDKLGFQGNTADYYDPRNSF
                     ITSLLRTGKGIPVTISVLCILIARRVGLPVSGVNMPGHFLLTFQEGDLVAYMDPFNSG
                     AILSHEECLQFLTRQNLPSDPAYLRIADSMTILKRMYRNLIHIHSSRGNREEERQLRR
                     HLFILENAGAASP"
     gene            4239..5168
                     /locus_tag="PLG78_10550"
     CDS             4239..5168
                     /locus_tag="PLG78_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE23601.1"
                     /translation="MTGGPVGQIERKLASIIGQDLPVLKKIKKYVIQSGGKRIRPLTH
                     YYTLRMLGSDHKEWVDAGAIGELIHNASLLHDDVVDESDLRRGKPSAPVLFGNKTVVL
                     AGDYLLACGLHHLSTLEHSRELLVIFSRTIRLLSEGELIQMEWEGNLKIPPKAYERII
                     YCKTGCLFGAMTESAAVLAGADSARAQYRAFGERLGRYFQVRDDYLDYFTPAKETGKK
                     EMQDFERGLVTWPVMRLLETKKGADKILRDIWKDRKKNLKKMLGLLEERRIQESSRAY
                     LKSELDSMLGFILKHPPSSFRDKMHSTLKTLEL"
     gene            5165..>5616
                     /locus_tag="PLG78_10555"
     CDS             5165..>5616
                     /locus_tag="PLG78_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23602.1"
                     /translation="MSQPMDSSPKVLRTVTAIRYALPLREGGSLPGLMEADDDGLYVV
                     KFRGAGQGAAALVSEVFFGEIARSLGLPIPEIVLVDLDVDISAAEGDPEIQDLLRSSA
                     GLNLGIDFLPGAFPFAPPIARALAADIVWFDSLVMNVDRTAKNPNLLR"
BASE COUNT         1214 a         1387 c         1453 g         1562 t
ORIGIN      
        1 cgcgataaag gagtgatcct ctgaatcccg gaaccgttga gcctttcagc atccacgcag
       61 gcatcctgga cattcgtttt cgcgtctacg ccgggcatct gagtctggcg caagagctaa
      121 agagaaacgg agaaaaagag catctctccc tttgtctcaa tcagggcgat tacacaaaag
      181 cagcttactt tctgaaaaca gacgaacgca ttcgcgtgct tttgatttcc ctcgagcaac
      241 cgggttctgc agcaaaggaa ggaatttccg aagcgatcgc gtatgcggca ctcgaatcgc
      301 ccaggcttgc ggacgtcatt gcatccgttc acaaagtggc gtttgaaaaa ggtggaatcc
      361 ttcgcgccgg agtgtatgag ctgcgcgaag acgaagcgcc tggggaagtg ccgggactct
      421 ttcatgccgt gactgcagga cttccacctc ccttgctgat ccgcgggggt gagtttacat
      481 tccctctccg gtctggaatt cctgccggga ttgtatctga atgggtctcc cagactcaga
      541 gcatggaatg gcatgcagga gacgcactgt acctctacac agattcagca gaggtagaag
      601 cttcccacga tttccatgaa tatcttcgcg cgcacaaagg cggttctctc caaattccgg
      661 aagatatgat agtatttcgc atcgaatttg gctcctgata aaataccttt gatcttttga
      721 ccacggcctt ctgagtgggt tcatgtggat gtggcctgct ttgatcgtct ctattgtcct
      781 gggtgcaatt ggacagatac ttatgaaaat tggaatgaag gcggcggggc ccgttccact
      841 ggatcgtgga atagttgatc ttttttacta tttcatgcat gcggtgctct cgttgccttt
      901 gatcggggcg gctctttcct atggagtcag ttttgtgttg tggctgggtg ttctttctgt
      961 agcagacttg agtctggccc gtcctatcat gtctgcaggc tatattctca ctcttgccta
     1021 cggctttgct tcgggtgaag atatcactcc cgcgcgggtt ttaggaactt gcctgattgt
     1081 agtgggcatt gcgtttgtag caaaaagtgg gattagaaca tgaaggctgc cgtactcgaa
     1141 aaagacgcac gatttctcca gataaaagaa gtagagaggc cagcactcgc tgcgggtatg
     1201 gtgcgcgtga aggtgcatgc atgcggtgta tgcggatcgg atgttcatct ggtgttgcac
     1261 agaagcatgc acgccaaatc ctatccaaga attcccggtc acgaatcatc cggagtagtc
     1321 atcgaagtag gagaaaatgt aaccagggtg aagcctcagg acagagtggt tgtttctgct
     1381 ggaaccagct gcggtcattg tgctgcctgc aaggcgggtc gcgaaaatct ttgcccggaa
     1441 gtcggagtcc tggggttcga ttcggatgga tcgtttgcgg aagaagtagt ggtgccagaa
     1501 cgttgtctct ttgttttttc ctctcacatt ccttttgagc aagcagccat tttagcggac
     1561 gcggtatcca ctccctatca tgcacttcga tatgcgggga acatcaagca aggagatact
     1621 gtggcagtgt ttggctgtgg tggccttggc attcacgggg ttctactggc gcgcgctctt
     1681 ggcgcgtcta gagtgtttgc gctcgacgta gaagcaggcg ctctctctaa cgcagagaaa
     1741 gcaggggcca atgaaatcat agatttgtcc ggaaagtcaa gcgttggcaa gatgaacgcc
     1801 ggcaaaattc taaaagaaaa aggcggagtg gacctggttg tagatttttc aggatactat
     1861 agtaatatag aggaatccgt tcgcgcaatg aatcccggcg gcagaatggt gatggtcgga
     1921 ctgggcagaa atccgctcaa gtttgcaatt ccggccactc tcatcttcaa gatgatcagt
     1981 gtatgtggat cgtatggatc ggattcgcgc gctctgccag agttgattga ccttctcgaa
     2041 aaaggaaaaa tcaatctggc gccttccatt acttctacgc atccattgga agaagcccag
     2101 gagtgcattg agaagctgga aaacagaaaa ggcaatccaa tccgatttgt aattcgtccc
     2161 tgaacgtggc ggccttactc actcttgcac tggtctggtt tgttccattc gacgcctctc
     2221 ttgtgaaggc ggcgctggtc gcactggcgc tcctggactc tttttttcca gtcacctctc
     2281 gcagcagaat gatcatcgtt tcatggattg ttctttcaca gttgattcta gcgtttactg
     2341 gcttttactt ttcagacgat agcctccgcc atcttcacga tggcttctat ttgcttcgag
     2401 gaatagacat ctatctgttt cctcccgcgg ctctgccggt ggtcgtgccc acaccggact
     2461 accatcgcat gatcggaagt gtctattttc cagttgttca gttcatgtct atgattggct
     2521 ccttgttttc tgtatcgcgt ggctatgtct ttgtatttca cggactctgt tctctgttat
     2581 ttggaatcct ggcgatgcga attcgtggaa gagtgttgtc tttcttctct cgttttgttt
     2641 tttctccagc atttgtgctc attgcaagtg gtcggcatga ggacttgctt gcggccgcga
     2701 ttttttttct ctctttgacc ctccactcac gaggtgcagg cttcttcact ggacttcttc
     2761 cctttatcaa gcctgacgca gccttcctct ccttgcttac gatgagaaag aatgccgcct
     2821 atctcctggc tatgattgtc agtgcttctt gcttgctctg gttttccttt caatttctgg
     2881 taatcagtga tctaacactt ccggcctttc tatccaatct ccgcttcttc gcagactggt
     2941 acgtgggata caatcctctg agtcttccgg gccctctgtt ttttgttcga attcgtccgt
     3001 ggattgctct ggctgcattt ctggcaggct tagtcttcat ctggcgtgca gcgtcttccg
     3061 gcccttccag gcgtttcgtg tccattctgg cggccacggg tgcggccgga gtcctgttca
     3121 gttttgctct tcgcggtgtc tggcatccat ggtacttttg ctctgcaatt tccgcgctca
     3181 tcttcttcaa aaaaagaaaa cttgcacaaa gtattccgcc ctatctcgtt ttattcttcc
     3241 tcccaattgc cgatcttcgt gcgggagggg gctttcactt tggcggattt tatgtcgcag
     3301 ttctcgggtt gctggtattg catctcgggc acaaattctt gacacgtcga aacagtgatc
     3361 gcctctgaac ttatgagccg ggaaatcgag agtctccttc tttctctcta tgactcctcc
     3421 gatgaaaagg agtcagcgta tcttacgaaa gaactctttc gcacgattcc tattggctat
     3481 cacccactgc gttacgtgga agcactcagc actcctgcgg ttcgctggaa acttcgcacc
     3541 ggcctttttc cggcgcttgg agatctgaag attcgccacg atcttaaaga gcttctgaac
     3601 cgcagccgcg aagacggaga catggatcta gagatgggag cattcctcat cgcacgtgga
     3661 gtggaggaac cggaagtagt gccggaagac ctgactgtgc agctcgacgc gcttgcgaag
     3721 cctcttgccc gggccatcaa gtatgcgggt ggagatccgg agagccgttt gaatttgttt
     3781 agattctata tgtttgataa gctgggcttt cagggaaaca cagctgatta ctacgatcct
     3841 agaaacagtt tcatcacttc tcttcttcgc accggaaaag ggatacccgt gacgatttct
     3901 gttttatgca ttctgattgc gcgtcgcgta ggcctgcctg tctccggagt caacatgccc
     3961 ggacactttc ttctgacgtt tcaagaaggg gacctcgtgg cgtacatgga tccatttaac
     4021 tccggagcca tcctgagcca cgaggaatgt cttcagttcc tcactcgcca gaaccttcct
     4081 tctgatcctg cctacctgag aatcgcagac tccatgacca ttctcaaaag gatgtatcgt
     4141 aacttgattc atattcactc gtcgcgcggc aatcgtgagg aagagcgcca gttgcgccgg
     4201 catcttttca ttcttgagaa cgccggggca gcctcgccgt gaccggcgga ccggtcggtc
     4261 agattgaacg gaagcttgct tctatcattg ggcaggacct tccggttcta aagaaaatta
     4321 agaaatacgt aattcaaagc ggcggaaagc gaattcgtcc gcttactcac tattatacgc
     4381 tgcgcatgct tggctcggat cacaaggagt gggtggatgc aggcgccata ggagaactga
     4441 ttcacaatgc ttctctcctt catgatgatg tagtggatga atcggatctt cgccgcggca
     4501 aaccttctgc acctgttctt tttggaaaca agacagtggt tctcgccggg gactacttgc
     4561 ttgcgtgcgg tcttcatcat ctgtccactc tggaacattc acgcgagttg ctcgtaattt
     4621 tttctcgtac gattcgcctt ctcagcgagg gagaactcat tcagatggaa tgggaaggaa
     4681 atctaaaaat tccaccaaaa gcatacgaaa gaattatcta ctgcaaaaca ggttgtctgt
     4741 ttggagcaat gactgaatcg gcggccgttc ttgccggcgc ggattctgct cgggcacagt
     4801 accgcgcgtt tggcgagcgt ctgggaagat attttcaagt gcgcgatgac tatctggact
     4861 acttcactcc agcaaaagag actggaaaaa aagagatgca ggattttgaa agaggcctgg
     4921 tcacatggcc cgttatgcga cttttggaga caaaaaaggg cgctgacaag attcttcgcg
     4981 acatctggaa agatcgtaaa aagaatttga aaaagatgct gggtctgctc gaagaaaggc
     5041 gcatccagga atcgtcgcga gcttatctga aatcagaact ggattccatg cttggtttta
     5101 ttttgaaaca cccgccttct tcttttcgcg acaaaatgca cagcaccctc aaaacgctgg
     5161 agttgtgagc cagcccatgg actcttcgcc caaagtactt cgcaccgtta cggccatccg
     5221 ctatgctttg cctcttcgcg aaggtggctc cttgcccgga ttgatggagg cagatgacga
     5281 tggtttgtat gttgtaaaat ttagaggagc aggacaggga gcggcggctc tcgtctctga
     5341 agtcttcttt ggagaaattg cgcgctccct gggccttcct attccagaaa tcgttcttgt
     5401 ggatctggac gtggatatct ctgcggcgga aggagatcct gaaattcagg atctgttgcg
     5461 ctcgagcgcg ggcctgaacc ttggaattga ttttttgccc ggagcttttc ctttcgcgcc
     5521 accaattgcc cgtgcgcttg cggcagacat cgtctggttt gattctctgg tcatgaatgt
     5581 agatcgcacg gccaaaaacc ccaatctact tcgctg
//
LOCUS       DAOQJL010000219         5575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8414_length_5575_cov_3.715942, whole genome shotgun sequence.
ACCESSION   DAOQJL010000219 DAOQJL010000000
VERSION     DAOQJL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8414_length_5575_cov_3.715942"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="PLG78_10560"
     CDS             complement(<1..1646)
                     /locus_tag="PLG78_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23603.1"
                     /translation="MRRVIILIAFAALAAPSALFAQLYTPPGYGAGQSVQGMGFGLDL
                     GYETVDDDIYVTVAPLLELPFFFDIKLGVQIPLEVLAYDRDPKSGKKTGSLRTGTFDD
                     TSDYIKVIKYARRGTHLYYNPGDAFNWSFFYGQMTDGYIGHRTIVHRYVNNYDPTVFK
                     AGLMADINNDWGGLEAFMSDVYRKEAVAGRAYIRPVGIVTGVHDIFWASSGFRPSQVA
                     LSVADNRDPHVNGGVFFQEEADKKMLEPGRGGRLQQHLYPTMRDSVDQGGTTGANTTS
                     ANNPSNPNRPIQFEKVTDPITGETQVRAVPADNPATNPANNPATNPANNPATNPANNP
                     ATNPVAPADSGKSESSKGSKWSPSFWNRWALGYTIARDKNAPLTLEKDGSANLVVDPD
                     DKRPRAATDQTLTIVGMDTEFRLSPFTFLEVTPYADLNKFKDIDKSKGLHGGVNFEFR
                     IMETLKLYIRPEYREMSSNYIPTYFDQYYSLERTALQPRGVSDFSGLSSASNQTKLAY
                     LKSLPVGGTKVRGYYGQVMMDWLQTLVVELNYEDYVGPDNS"
     gene            complement(1699..3306)
                     /locus_tag="PLG78_10565"
     CDS             complement(1699..3306)
                     /locus_tag="PLG78_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNE23604.1"
                     /translation="MNFRLLSEYPELSSEERSLCHMLIQVETPPVTDSPTRRPLVVAI
                     AIDKSKSMYGEKMQATLEAASSLVNWLTRHDQLAIIAYDTNVEVVQPLTPLSDKFSVI
                     KKLENVRVGTATNLSGGWLQALRSVEAADVENAFRRVILLTDGMANTGIVSTPELVKI
                     AEDHFSRGISTTTIGFGRDFSELLLRDVAKAGGGNFYFIDGPEQAQEVFFREFGDIAA
                     LFGQGLEIKLKTSPGVQVKEILNDMPFQMQDDGSVILRPGDLRSDDLRNIVVVLEIDG
                     KVAAPYSEGLLGAELSFYNLVHGSRLERFDAGVAMNFKARPSDGYNKTVRLEALTAAS
                     ARAMIEASRVSGERDLSAAKQILGRMVRRLQENEATEPDQVRKLIRRLEEMERNLEEN
                     MNLARKNMMAGGAELGRITSGEYYFSGTAHDKVAELSLSGQLDLYKCPDLKGDMRRLM
                     EQGYRFVVFDLTDLSYVDSSGIGTLIQVSNWMKKRGGLMVLVNVQGSVEKIFQLSKLD
                     EFFAFRDTVSEARQFLEELIAQGRQGR"
     gene            complement(3363..4022)
                     /locus_tag="PLG78_10570"
     CDS             complement(3363..4022)
                     /locus_tag="PLG78_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23605.1"
                     /translation="MRRLLIVIWIVLCAPLIAEKEDESKLAYESGIAYSMAGDAAKAK
                     AQYEKAASVKGDFSDLARVELLRLQARDGKTLIAPLTQMLSQIKDQTLQEQAYLALAG
                     SLKESRRYQDSIDVCMLLVGRMPESELADDALLLSARIFFDLGQFDSARSQAEQIVAR
                     YKKSNSLDSARLLLAELYLIHDENYSPALACKFYASAARDSQAILTPVDSNLSRICSE
                     R"
     gene            complement(4022..4645)
                     /locus_tag="PLG78_10575"
     CDS             complement(4022..4645)
                     /locus_tag="PLG78_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23606.1"
                     /translation="MNSLFALIDRMTAKLPPNVLRLIRLGAILVWTVGATVVVFFSWK
                     KGQDATPAQGQDLSIATIKERVQKEENRRIRGDVTVPDLGEFLPESSSPDLPRQRKEN
                     HKQGLSGVDDRLIDPENPIEKPSALPPFLGEDARGETPLRLPDTRPREDAKRDDMRRS
                     DRSREGGPREDKPRENLRDAGRSNDRRSDMPAPARRDRQKPDLMPVE"
     gene            complement(4685..>5575)
                     /locus_tag="PLG78_10580"
     CDS             complement(4685..>5575)
                     /locus_tag="PLG78_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE23607.1"
                     /translation="ANPAYFYYLSRRLFTGLRRPGYMILEVSPESFNRNTGILGTTIL
                     NHGIAGGYMEPMSTMIPQMDKEAYNDTRAFAFYGLPFSLREAILRVRGSISPEKKKSG
                     SMFDLIPIEQMEKGFQAIYGPAVEWQSGLMMSMKNAEIHNLDLYSMEQSHEVKFLNLM
                     NGAQYAWFGTATDAELKSDTDRIAGIYLKRFQVSEEQVVFFERTLDAARAAGVKVIVF
                     WPQVNPYLETRLNADPRFAILKKRIADESKDRGAVVLDMSAQEETRCKNFYDASHLSI
                     QCFPAITGFLLDRLEKVPSN"
BASE COUNT         1309 a         1468 c         1399 g         1399 t
ORIGIN      
        1 gaattatctg ggccaacgta gtcttcatag ttgagttcca cgaccagagt ctgcagccag
       61 tccatcatta cctgaccgta gtaaccacgg accttcgttc cgcccactgg cagacttttg
      121 agatacgcga gttttgtctg attggaggca gaggatagcc ctgaaaaatc agacactccg
      181 cgaggctgca gtgccgttct ctcgagcgaa tagtactgat caaagtaagt tggaatgtaa
      241 ttcgaactca tctcgcgata ttccggccga atgtagagct tcaatgtttc catgattcga
      301 aactcgaagt tcactccgcc atgaaggcct ttgctcttgt caatgtcctt gaacttattc
      361 aggtccgcgt agggagtcac ttcgaggaat gtgaaaggag aaagcctgaa ctctgtgtcc
      421 atacccacga tggtcagagt ctggtctgtg gccgcacgag gacgcttatc gtccgggtct
      481 acaaccagat tcgcggatcc atctttttcc agagtcaacg gcgcattctt atcgcgcgcg
      541 atcgtgtagc ccagggccca tctgttccag aaactcggag accatttgga tcccttgcta
      601 ctttcgcttt ttccggaatc cgcaggcgcc acaggattgg tcgcagggtt attcgctgga
      661 ttggtcgcag ggttattcgc tggattggtc gcagggttat tcgctggatt ggtcgcaggg
      721 ttgtccgctg gaacggcacg cacctgagtt tcgcctgtga tgggatctgt tactttttca
      781 aattgaatcg gtctgtttgg attggaggga ttgttcgcgg aagtagtatt cgctcccgta
      841 gttcccccct gatccacact gtctctcatg gttggataga ggtgctgctg gagtcttcct
      901 ccgcgtccgg gctcgagcat ctttttgtcc gcttcttcct ggaagaagac gccgccgttt
      961 acgtgaggat cacggttgtc tgccacagac aacgccacct gcgaaggacg aaagccagag
     1021 ctggcccaga agatatcgtg aactccagtt acaattccaa caggccgaat gtaggctcgc
     1081 cccgccactg cttctttgcg atagacatcg gacatgaacg cttccagacc accccagtcg
     1141 ttgttgatgt ctgccatgag ccctgctttg aatacagttg gatcatagtt gttcacgtat
     1201 ctatgaacaa tcgttctgtg accaatgtag ccgtccgtca tctgcccgta gaagaaagac
     1261 cagttgaatg cgtctccagg attgtagtaa aggtgcgttc ctcgacgagc atacttgatc
     1321 accttgatgt agtcggacgt gtcatcaaag gtcccggtac gaagagatcc tgttttcttt
     1381 cctgattttg gatcgcggtc gtatgcgagg acttccagtg ggatctggac tccaagctta
     1441 atgtcaaaga agaacggaag ttccaggaga ggcgctaccg tgacgtaaat atcatcgtct
     1501 acggtttcgt agccgaggtc caggccaaag cccatgccct ggacgctctg gcccgcgccg
     1561 tatccaggcg gagtgtagag ctgggcaaac agcgcagacg gagctgccag tgctgcaaaa
     1621 gcaatcagga tgatgacgcg acgcaattct tcctctagta tcttcgatac ggaaagtacg
     1681 ggcaaaaaat gaagtaaatt aacgaccctg acggccctgg gcgatcagtt cttccagaaa
     1741 ttgccgcgct tcagaaaccg tatcgcgaaa tgcgaagaac tcatccaatt ttgaaagctg
     1801 gaagatcttc tcaacactgc cctgaacgtt gaccagaacc ataagccctc cccgcttctt
     1861 catccagtta gaaacctgga tcagggtgcc gataccggac gaatctacat acgagagatc
     1921 cgtcagatcg aacacgacga agcgatatcc ttgctccatt agacgacgca tatcgccttt
     1981 tagatcagga cacttgtaaa gatcgagctg ccccgataga ctgagttccg ccactttgtc
     2041 gtgggccgta ccgctgaaat agtattcccc ggaggtgatt ctccccagtt ccgcgccgcc
     2101 tgccatcatg ttcttgcggg caaggttcat attctcttcc agattccgtt ccatctcttc
     2161 cagacggcga atgagtttcc gcacctgatc tggctcagtg gcctcattct cctgaaggcg
     2221 acgcaccata cgaccaagga tttgtttggc ggcagaaaga tccctttctc ccgaaacccg
     2281 cgatgcttca atcatggctc tggcactcgc tgcagtcaaa gcttccaggc gaacggtctt
     2341 gttgtatccg tcagaaggcc tggccttgaa attcatcgcg accccggcgt caaatcgctc
     2401 caggcgagat ccatgtacaa gattgtagaa agaaagctct gcccccagca agccttcgct
     2461 gtatggtgcc gctacctttc cgtcgatttc aagaacgact acgatgttgc gaagatcatc
     2521 cgagcgaaga tctcctgggc gaaggatgac agaaccatcg tcctgcattt gaaacggcat
     2581 atcgtttaga atttctttta cctgaacgcc gggggaagtc ttgagtttga tctcaagacc
     2641 ctgaccgaag agtgccgcaa tgtctccgaa ttcgcggaag aaaacttcct gcgcctgctc
     2701 tggaccgtct atgaaataga aattccctcc gcctgccttt gccacatcgc gcaaaagcaa
     2761 ttcagagaag tcgcgtccaa atccaatggt tgtagtggag attccgcgcg aaaaatgatc
     2821 ttccgcaatt ttcacaagct ccggagtaga aacaattcca gtgtttgcca tgccgtctgt
     2881 aagcaagatc acacggcgaa atgcgttttc aacgtccgct gcttccacag aacgaagagc
     2941 ctgaagccag cccccgctca aattcgtggc agttcccact cttacgtttt ccagtttctt
     3001 gatgactgag aatttatccg aaagaggagt cagaggttgc acgacttcta cgtttgtatc
     3061 gtatgcaatg atggcgagtt gatcgtgacg ggtgagccag ttcaccagag aagaggccgc
     3121 ttccagagta gcctgcattt tttcgccata catggattta cttttatcga ttgcaatggc
     3181 cacgacgagc ggacggcgag taggcgaatc tgtgacaggt ggtgtctcca cctgaatgag
     3241 catgtgacag agacttctct cctcgctgga gagttccgga tactcggata gtagacgaaa
     3301 gttcatatca cctgccccag acttcaattt ccggggggcg aacaaaagat caagtatttc
     3361 cgtcagcgct cagagcaaat acgagacaaa tttgaatcga ccggagtcaa aatggcctgc
     3421 gagtcgcgcg ccgcagatgc gtagaattta catgccagag cgggcgaata gttctcgtcg
     3481 tgaatcagat aaagctcggc caggagaagg cgggctgaat ccagggaatt ggacttcttg
     3541 tagcgagcca cgatttgctc cgcctgggat cgcgccgaat caaactgacc cagatcaaaa
     3601 aagattctgg cagaaagcaa tagagcatcg tctgccagct cactttccgg catccggcct
     3661 acaagcagca tgcatacatc gatggagtcc tggtatcttc tagattcctt cagacttccg
     3721 gcaagcgcaa gatacgcctg ctcctggagg gtctggtctt ttatctgcga aagcatttga
     3781 gtgagcggag caatgagagt cttgccatcg cgagcctgca gccggagaag ttctacgcgc
     3841 gccaggtctg agaagtcgcc cttcacggac gctgcttttt cgtactgagc ttttgccttc
     3901 gctgcgtcac ctgccatcga atatgcaatg cctgactcgt aagcaagctt actttcatct
     3961 tctttttcag cgatcaacgg agcacagaga acgatccata ttacaatcaa aagtcgtcgc
     4021 atcactctac cggcatcaga tctggcttct ggcgatctct tcgcgcaggt gcaggcatgt
     4081 cgcttctcct atcattgctt cgacccgcat cgcgcaaatt ctccctgggc ttgtcttcgc
     4141 gaggtccgcc ttcgcgcgac ctgtcagatc gccgcatatc gtcacgcttt gcatcttcgc
     4201 gcggtcttgt atccggtagg cgaagaggag tttctccgcg tgcgtcttcg cccaggaatg
     4261 gaggaagagc agaaggtttt tcaattgggt tttcaggatc aatcaagcga tcgtccactc
     4321 cagagagtcc ctgtttatga ttttcttttc tttggcgcgg caggtccggt gaggatgact
     4381 ccggcaggaa ttctcccaga tctggcactg ttacgtcccc acggatccgg cgattctctt
     4441 ccttttgcac gcgctctttg atggtcgcaa tggatagatc ctgaccctgg gctggagtcg
     4501 catcctgtcc cttcttccac gagaagaaca caaccacagt tgcacccacg gtccagacca
     4561 ggatggcgcc caggcgaatc agcctgagca cattgggagg cagttttgcc gtcatccggt
     4621 cgatcagggc gaacaatgag ttcatgatgg tattatcgtt aagatttgga agagtgtgga
     4681 caatttaatt agacggaact ttctccaatc ggtcgagtaa aaagccagta atcgcgggaa
     4741 aacattgaat ggataggtgg cttgcgtcat agaagttctt gcagcgagtt tcctcttgag
     4801 cgctcatatc cagaacaact gctccgcggt ctttagactc atctgcgatt ctcttcttga
     4861 gaattgcaaa gcgcggatcc gcattcaggc gagtttccag atacggattt acctgaggcc
     4921 agaacacaat caccttcact ccagcagccc tggccgcatc gagtgttctc tcaaaaaaca
     4981 caacttgctc ctctgaaacc tgaaatcgtt tcaaatagat gcccgcaatt ctgtctgtgt
     5041 ctgatttaag ttctgcgtct gttgccgttc caaaccatgc atactgcgcg ccgttcataa
     5101 ggtttagaaa ctttacttca tgagactgct ccatgctgta aagatccagg ttgtgaatct
     5161 ccgcattctt catggacatc atcagaccgc tctgccattc caccgcgggt ccgtagattg
     5221 cctggaagcc cttttccatt tgctcgatgg gtatgagatc aaacatactt ccggatttct
     5281 ttttctcggg cgaaatcgat ccgcgaacgc gaagaatcgc ttcgcgaagt gaaaaaggca
     5341 gaccgtaaaa agcaaatgct cgcgtgtcat tgtatgcttc tttgtccatc tgcggaatca
     5401 tagtggacat tggttccata tagccgccag caattccgtg attgagaatc gttgtgccca
     5461 gaatgcccgt gttcctgttg aaagactctg gggaaacctc aaggatcatg taacccggtc
     5521 tgcgaaggcc tgtgaaaagt ctgcgagaaa ggtagtagaa ataagccgga tttgc
//
LOCUS       DAOQJL010000220         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8428_length_5567_cov_4.476234, whole genome shotgun sequence.
ACCESSION   DAOQJL010000220 DAOQJL010000000
VERSION     DAOQJL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_5567_cov_4.476234"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLG78_10585"
     CDS             complement(<1..1141)
                     /locus_tag="PLG78_10585"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNE23608.1"
                     /translation="MGLKYYERFTPRVSYRETQDGKLITESFDSMGVVRDSELKTFRW
                     APSEKNEVLSGTLRLTRNKPVYLERFELVLAADEKKSPFVSLHDDISPDSGLRLFQHG
                     YHSWSWTGARQADETDNICLLKWKHNLDENPETQINSALPFSLPINVFPRKGKFHSEF
                     LVLLETNTKFLKKKNGKCILFSTYGRGDQFLKFRIHLDPKTAKLEEFAIVWDGAGMHM
                     PGSTIITLTQVKHFNSIHVRPRTRVRLKRTQPSPAEFLESCAKDVAGEFKTPKMENSV
                     TGWCSWYYYYNKISEGVLLRNLRLVKEKKLNIDFFQIDDGWQKNIGDWLKTAPEFPSG
                     MAFFSKAIKNEGLRPGIWLAPFIARPDSDVFKENKEFVLHDQHGDP"
     gene            complement(1193..2338)
                     /locus_tag="PLG78_10590"
     CDS             complement(1193..2338)
                     /locus_tag="PLG78_10590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNE23609.1"
                     /translation="MEKKPLLLIVDDDPNIRAILQNILAENHNLLHANDGIEGLGVAR
                     KSLPDLIISDVMMPGMDGFEFCASIRSDPFLRRIPILLLTGEHDLGSKLKGLHQRADD
                     YVTKPFHAEEIEARVSNLLEKRRLERELQQRLEEMQNDLALARNVQQQLLPVRIPPLP
                     NAHFETLYRPIAALGGDFFEFVVLPDRLGLFICDVAGHGIPAALVASMVKIVLANVAM
                     DAAGPGDLMVRLNRQMIQSTGERYVTAFYATLDLSTLQMQFASAGHIAPILVRNGALI
                     PLEAHGPLLGAFPKDHWGEEVVALQPRDRVFFYTDGVIDCRNKSEEHFGEERLNSCLL
                     AHQGATARFFLDQLYHEISHFVGDRNFADDITAFVIDLATRKGAVVS"
     gene            complement(2396..4165)
                     /locus_tag="PLG78_10595"
     CDS             complement(2396..4165)
                     /locus_tag="PLG78_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNE23610.1"
                     /translation="MEFHVSDFVVIAIYFIVSLGAGLLGSRNKSSLQEFFISGGRAPF
                     WLVGTGMVATTFAADTPLAVAGLVGKNGIAGNWIWWCSAAGGMLTVFFFARLWRRSGI
                     TTDLELIELRYSGKAAAILRGSKAIYFGLIINCIILGWVNLAMLKIFQGMIPGIDARW
                     LVTGSAIITALYVAVSGLWGVAITDAFQFAIAMIGCIVLALIAAFHPQIQAAGGIAHV
                     LPPSVYNFFPTFHSDPVQSADLSPLYTIPLTSFLAFILVQWWASWYPGAEPGGGGYIA
                     QRILSARDEKHGMLATLWFVIAHYCVRPWPWIIAGIAALALYPGLLPQEKESGYVYLM
                     RDLLPSPYRGLLIAAFLGAYMSTLSTHLHWGSSYLIHDFYQRFVKRNASERHYLLAAR
                     LCTILLLCVSLFVTFNLLETISGAWEILLEFGAGTGFVLILRWYYWRINAISEITAMI
                     VPAICVLLIHFLPRAFGEPESGSMLATLVKFPGNLFLIVSTTIAFTLLATFLTRPEPE
                     HVLQNFAKIVRPAGPGFKRFGGSGVLWPKFAGWISGTALTYAVLFLSGYLLFGMWERA
                     AYFSVLAIVSLIALIYSIQKDTA"
     gene            4407..5123
                     /locus_tag="PLG78_10600"
     CDS             4407..5123
                     /locus_tag="PLG78_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23611.1"
                     /translation="MGESLILGAWISANRWLTFNPSSIGGLRFWIRAENIQQSNGTSV
                     TSWADLSGTGNTVVAGTAPTYYSSTNTINSRPVVRFTGTAVLTNAAPVGLTSSDSGSI
                     FAVLRSNQASADILIIGGCGNTARQFQFSTTTISPTKTCVGQAINPAYSWPIGAMHEV
                     AWIQNLATYVEVRADGRLVASGVPAASGYGTGALNVSNSTASGMDLAELLWFDSALTT
                     QNITSVECYLGARYGTTTCL"
     gene            complement(5204..>5567)
                     /locus_tag="PLG78_10605"
     CDS             complement(5204..>5567)
                     /locus_tag="PLG78_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23612.1"
                     /translation="EGCERLAKGMSVVIFPEGTRSRIFQPSQFNSLGTKLAKRAGVCM
                     MPVALKTDFWGNGKLLRTFGPLDCRKQAHFKFGAPVMPEGNGKEAQEYALEFIQKHVQ
                     MWDRKPGQVVELPGFAAT"
BASE COUNT         1368 a         1392 c         1412 g         1395 t
ORIGIN      
        1 cagggtctcc gtgctggtcg tgcaagacga attctttatt ttctttaaag acatctgagt
       61 cgggacgtgc gatgaaaggt gccagccaga ttcctggacg aagcccttcg ttcttgattg
      121 cctttgaaaa aaacgccatg ccggatggaa attcaggtgc tgttttcagc cagtctccaa
      181 tgtttttctg ccagccatcg tcgatttgga agaagtcaat gttcagtttc ttttctttta
      241 cgagccgcag gttgcgaagg agcactcctt cgctgatttt gttgtaatag taataccacg
      301 aacaccatcc tgtgacagag ttttccatct ttggtgtttt gaattcgcct gctacatcct
      361 ttgcacacga ttccagaaac tctgcaggag aaggttgtgt tcttttcagt ctcactcgag
      421 tgcgaggccg cacgtgaatg ctgttgaagt gttttacctg agtgagcgtg atgatggtgc
      481 ttccgggcat gtgcattcct gcgccgtccc acacaattgc aaactcttcc agtttcgcgg
      541 ttttaggatc cagatgaatt ctgaatttga ggaattgatc tcctcttccg taggtggaaa
      601 acagaatgca ttttccattt ttctttttca gaaactttgt gttggtctca agcaacacca
      661 ggaactccga atggaatttt cctttccgcg gaaatacgtt gataggcaaa gagaatggaa
      721 gtgcactatt gatttgggtt tccggattct catccagatt gtgtttccac tttagaagac
      781 agatattgtc tgtttcgtct gcctgtcttg cacccgtcca ggaccatgaa tgatagccat
      841 gctgaaacaa tcgcaagcct gaatctggag atatgtcatc gtgcagggaa acaaaagggc
      901 ttttcttttc atctgccgcg agtacgagtt caaacctttc tagatacact ggtttgttgc
      961 gagtcaggcg aagagttccg ctcaatactt catttttttc agagggagcc cagcgaaagg
     1021 tcttgagttc agaatcgcga acgactccca tcgaatcaaa agattctgtg atgagtttcc
     1081 catcctgtgt ttcacgatag cttacgcggg gagtaaatcg ttcgtagtac ttcagaccca
     1141 tctgcacaga ggacgccagg ccatgcctgt tgtaaagaat ttggcgggcg cgtcagctga
     1201 ctacagcgcc tttcctggtc gccagatcaa tgacaaaagc tgtgatgtcg tctgcgaagt
     1261 tgcggtcccc gacgaagtga gatatttcat gatacaattg atcgaggaag aatcgcgcgg
     1321 ttgcaccctg atgggcgagc agacatgaat tcagtctctc ctctccgaaa tgttcttcgc
     1381 ttttgtttct gcaatcgatc actccatccg tgtaaaaaaa tacccggtcg cgcggttgca
     1441 gcgccacaac ttcttctccc cagtgatctt ttggaaacgc gcccagaagt ggtccgtgtg
     1501 cctccagtgg gatgagagct ccatttcgca ctagaatcgg tgctatgtgc ccggcagaag
     1561 cgaattgcat ctgtagagtg ctcagatcga gtgtagcata gaaagcagtt acatatcttt
     1621 cgcctgtgct ctggatcatc tggcgattga gtcgaaccat caggtctccg gggccagcgg
     1681 catccatggc aacattggca agaacgattt tcaccatgct ggccacgagt gcggcaggaa
     1741 tgccgtggcc tgcgacgtcg cagataaaaa gtccaagtcg atccggcaaa actacgaatt
     1801 caaaaaaatc accacccagt gcggcgatgg gacgatataa cgtttcaaaa tgagcgttgg
     1861 gcagaggcgg gattcgcacg gggagcagct gctgctgtac atttctcgca agagcgagat
     1921 cgttttgcat ttcttcaagc cgttgctgca actctcgttc cagtcttcgt ttctcaagca
     1981 agttggaaac acgcgcttca atctcttctg cgtggaaagg cttggtaacg tagtcatccg
     2041 ctctctgatg aagacctttc agtttgcttc caaggtcatg ttcgcctgtc aaaaggagaa
     2101 ttggaattct acgaagaaat ggatctgatc gaatgcttgc gcaaaattca aatccatcca
     2161 tgcctggcat catcacatca gagatgatca gatccggcag actctttcgc gcaacaccca
     2221 gtccctcaat gccatcgttt gcatggagca ggttgtgatt ttctgctaga atgttttgga
     2281 ggatggcacg aatgttcgga tcatcatcta cgatcaacag aagtggcttc ttttccatcc
     2341 tcaaaacaac caggtcactg tatacacccg gctgccgagg atcgtcaagc tgatctcagg
     2401 ctgtatcttt ctgaatcgaa taaatcaatg ctataagaga aacaatcgca aggacggaaa
     2461 aataggccgc tctctcccac attccaaata gtagatatcc tgaaaggaat aataccgcgt
     2521 aggtgagtgc agtgccggat atccagcctg caaatttagg ccagagaaca cctgagccgc
     2581 caaatcgttt gaatccaggg ccggccggtc tcacgatctt tgcgaaattt tgcagtacgt
     2641 gctccggctc aggtctggtt agaaatgtgg ccagaagagt gaatgcgatt gtagtagaaa
     2701 caatcaagaa cagattccca ggaaacttca caagagtggc cagcatactt ccgctttcag
     2761 gttctccgaa tgcacgcggt agaaaatgaa tcaggagaac acaaatggca ggaacgatca
     2821 tggctgtgat ttcagagatg gcgttgattc tccagtagta ccagcgcaga atgagaacaa
     2881 aaccagtgcc agctccaaac tcaagcagga tctcccaggc tcctgagatc gtctccagca
     2941 agttgaaagt cacaaacaaa ctgacgcaaa gaagcagaat ggtgcagagt cttgctgcca
     3001 acaggtagtg tctttcggat gcgttccttt ttacgaatct ctggtaaaaa tcgtgaatga
     3061 gataacttga gccccagtgc aaatgagtgg agagagtgga catatatgca cccaggaaag
     3121 ctgcgatcaa caaaccacgg taaggcgaag gcagcagatc gcgcatcagg taaacgtaac
     3181 ccgattcttt ttcttgagga agcagtccgg ggtaaagagc aagggctgca attcctgcaa
     3241 tgatccaggg ccacgggcga acgcaataat gtgcaatcac aaaccagagt gttgcaagca
     3301 tcccatgttt ctcatctcgc gcggagagaa ttctctgtgc gatgtatccg cctcctcctg
     3361 gttctgcgcc cggataccac gaagcccacc attgcaccag aatgaatgca agaaaactcg
     3421 taagaggaat cgtatagagt ggagaaaggt ctgcggattg aacaggatca gagtggaacg
     3481 ttggaaagaa attgtagaca gagggcggaa gaacatgtgc gattccacct gccgcctgaa
     3541 tttgcggatg aaaggcagcg atcagcgcga gcacgatgca cccaatcatg gcgattgcga
     3601 attgaaacgc atccgtgatg gccactcccc ataaaccaga aacggcgacg tagagcgccg
     3661 tgatgatggc gctgccggtt acgagccatc gcgcgtctat tcctggaatc atgccctgaa
     3721 aaatcttgag cattgccagg ttgacccatc cgagaatgat gcagttgatg atcaggccaa
     3781 agtagattgc tttggaaccg cgaaggatgg ccgccgcctt tccgctgtat ctgagttcga
     3841 tcagttccag gtctgtcgtg attcctgatc ttcgccataa gcgtgcgaag aagaaaactg
     3901 tgagcattcc tccagctgca gagcaccacc agatccagtt tcccgcgatt ccgttttttc
     3961 caacaagtcc tgccactgca agaggagtgt ccgccgcgaa agtagtggcg accattcctg
     4021 tgcccacgag ccagaagggc gctctccctc cgctgatgaa gaattcctgc agagaggatt
     4081 tgttccgact tccaagaagt ccggcaccga ggctgacaat gaagtagatt gcgatgacta
     4141 caaaatctga aacatgaaat tccatctggt gcaggcgatc attgggcctg tccctgtaaa
     4201 gaaaacatgg tccgcgcgaa gatttgtttg ttgaattcgt gcgaaaggca tacgatatat
     4261 ggaggaaatc aggacttgtg tttatgctgg aatcgtctcg cctttcgctt cgtaacttgc
     4321 gccattggcc tttggttggt cttctggcac tgggctttct gcccggctgc actccctcct
     4381 tccctgacaa ttctgctaat gagccaatgg gcgaatcttt gattctgggt gcctggatca
     4441 gcgcgaatcg ctggcttacg ttcaatcctt cctctattgg cggtcttcgg ttctggattc
     4501 gcgcagaaaa cattcagcaa tcaaatggaa catccgttac atcctgggcg gatctaagcg
     4561 ggacagggaa tactgtggtg gcgggcacag cacccactta ctattccagc accaacacaa
     4621 tcaatagccg gcccgtagtg cgcttcaccg gcactgcagt tttgaccaat gctgcaccgg
     4681 tcgggttgac cagttccgat tccggtagca tcttcgcagt cttgagatcg aaccaggcga
     4741 gtgcggatat tttgatcata ggtgggtgcg gaaacactgc gaggcagttt cagttttcca
     4801 caactaccat ctctcctacg aagacctgcg tggggcaggc gatcaaccca gcctattcct
     4861 ggccgatcgg agcaatgcac gaagtcgcct ggattcagaa tcttgctacc tacgtggagg
     4921 tgcgcgcaga cgggcgtctt gtcgcctcgg gagtgcccgc ggcttccggc tatggcactg
     4981 gagctttgaa tgttagcaat tcgactgcgt ccgggatgga tcttgcggag ctgctctggt
     5041 ttgacagtgc actcacaacg cagaatatca ccagcgtaga atgctatctg ggcgcccgat
     5101 acggaactac cacatgcctt tgagcgcgtt gatccgttag gcggcgttgc tcagctctgc
     5161 gacctggttt tttgcgctac agtcgttttg cgtggcgatc gggctatgtg gcagcaaatc
     5221 caggcagttc tacaacctgg cccggttttc tgtcccacat ttgcacgtgt ttctgaataa
     5281 actcgagcgc gtactcctgc gcttcctttc cgtttccttc tggcatcaca ggagcaccaa
     5341 acttgaaatg ggcctgcttg cgacaatcga gcgggccaaa tgtccgaagc aatttgccat
     5401 tgccccagaa atcagttttc aatgcaacgg gcatcatgca cactcccgct ctcttcgcga
     5461 gctttgtacc cagtgaattg aactgggacg gttgaaagat tctgctgcga gtcccttccg
     5521 ggaagatcac gactgacatt cccttcgcga ggcgttcgca tccttcc
//
LOCUS       DAOQJL010000221         5551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8461_length_5551_cov_4.374636, whole genome shotgun sequence.
ACCESSION   DAOQJL010000221 DAOQJL010000000
VERSION     DAOQJL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_5551_cov_4.374636"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3248
                     /locus_tag="PLG78_10610"
     CDS             <1..3248
                     /locus_tag="PLG78_10610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE23613.1"
                     /translation="FLIAFFTFLLVGGGLAAAFVAALFVTPPLPDFQHKRNVDKRVRI
                     LRDQAQDLSAKQKHGHEVQRKVEDKKGPLLAIPRQGDKLVTAFLVQHDLASVAAFRDH
                     KDRIDVVFPDWYFLTSAKCEVSERIDEEVRRVLDESDSAVIGRVTNGDHEKWHTAEMR
                     RLLASPQLRECIAGVLVQFAERDHLKGLNIDIETLGTAGRADYVKFLKELADRLHERD
                     MILTVDITAHDPAYDVKAIAQVADGVVLMAYDEHYTSGPAGPIASQEWFEENVAFTLK
                     DVPPEKLIVGIGTYAIDWSQNHSYSMKFSEVMALADDMGALPGMDPESRNMHFSYSED
                     GNNHTVWFLNNVTAWNQKLAIDRLGVAGIGVWRLGAEDPSIWKILPQQKPNIEAVVEP
                     EALRSVLYLSFGEVFHLHTVPSRGKMSVEQDGEGFIDAAKYDSLPEGYLIERIGNSIP
                     DKSLVLTFDDGPDPVWTPKVLDALKESKVHATFFVVGEQAERYPEIVQRILAEGHAIG
                     NHTYFHPDLSKTSPERVRLELNSTQRIIERETGYKTMLFRAPYNTDSNPDEAPEIDPL
                     RTAADMGYLIIGANIDVADWQKPTARLMADRLIEQAKNPRNHIIVMHDGGGDRSRTVE
                     ALRIGIPELKSKGYVFQSLEDATGISKSTLFPAMPASEAMLVYITAASWLSIRWGWVI
                     ITWLFLFTTLLAIFRIVFLGILVFKSVRKAEERKDDGTFLPPVSVLIPSYNEAKVLSQ
                     TIQSLQQSTHPQFEMLVIDDGSTDNTKEIVRGLMKKDKRIRLVSQPNGGKSSALNLGM
                     KEAKHEIVVTIDADTIVMPQTLTELAAPFKDPLVDAVCGNVEVGNVHNVLTGFQSLEY
                     ITSQNFDRRAFDALNCISVVPGATGGWRRSKVLEAGGYKSDTLTEDADLTLTMLENGA
                     RIVYAAEARSRTEAPATIRTLSKQRFRWSFGTFQCLWKHRHAFFKGPLGWVALPNMFL
                     FQVLFPALSPIGDVVFILSILRGDMEAIAVGYLLFLFMDVCGSLLAFILENKRKRLMF
                     LVLIQRFFYRQFMYIITFKAMFAMLKGARYGWNKLERQGTVRMNPELAKSH"
     gene            complement(3245..4963)
                     /locus_tag="PLG78_10615"
     CDS             complement(3245..4963)
                     /locus_tag="PLG78_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE23614.1"
                     /translation="MYNTSLALATRAARFRSRLTFGALVFFAFFRLILLPEGEGLPLF
                     SSWLADSAVFLTLLLIARILQAIPGGRIVSRLFFHSAYLLYFVLTFSYCYFFREATIR
                     QYSIFDLTLSGIAYFFTNVLPFWGNILLLAALLLVYVVSYLRDDWHIPTTLAFLLCGS
                     LFMVAKATANENASPILHLIQDVSEAARSPGLTVDPAPTEFSLDDLDSWTHPAPRFEP
                     RFQKIIVLVMEQITLAQLREDSRALPHGTFFNREEEHSHEYNNFFAADMDSRTGMLAL
                     LGGRLIPFEAYSDEDVDQYSFVSGKPSLLNIVKNRNYRTVYSASETERELVVTDLPWD
                     QIYTLTHEEVDQARSKYLCINPYEFEHSCEDKIMVSRLVNDVVKHDRVFLFYEFIFGH
                     ASEYIDIQKKTAVQYYSEFIDDLISGLRKAHELDHTLIVVTSDHGIRDHGYESWLSTY
                     RIPLLFYHSSFKKQLHPELHSQIDFRDIFLEESSGKPSSLKSRKFAVFMGTTTSSIVG
                     AITDQGDVMVLKNRKWLPYVMFNKNADEEDAPGGPPKSDLKPGAVVRYIQDYRAKFLS
                     GNLPAH"
     gene            complement(4997..>5551)
                     /locus_tag="PLG78_10620"
     CDS             complement(4997..>5551)
                     /locus_tag="PLG78_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23615.1"
                     /translation="FLTPLTDYQAADLKNMLLHPAGLPALEKRYATLLTHEKSEIARD
                     LNGALPHLRAEQLEAHLASMREDERKTLFAFFVSRVVSSLLADQIVRYRGILRDNAKR
                     EILILLYEPVHSSSHYYGRLLDFFAKEKPGYNLRLAMAYKRPPPRRRENLLHPFVEAL
                     RDLENKKYIESAEPLIPDDSSPRD"
BASE COUNT         1426 a         1283 c         1485 g         1357 t
ORIGIN      
        1 cctttctcat cgcattcttt acattcttgc tcgtgggcgg tggactagct gccgcgtttg
       61 tagccgcgct ctttgttact cctcctctcc ctgattttca gcacaaacgc aatgtggaca
      121 agagggtcag gattttgcga gaccaggcgc aggatctgag tgcaaaacag aagcatggcc
      181 acgaagtgca gcgcaaagtg gaagataaga aaggtccact tcttgcgatt ccccgtcagg
      241 gagataagct ggttaccgcg tttttagtgc agcatgatct ggcgagtgtg gcggcatttc
      301 gcgatcacaa ggatcgaatc gatgttgtgt ttcctgattg gtactttcta acaagtgcca
      361 aatgcgaagt gagtgaaaga attgatgaag aagttcgtcg cgtcctggac gaatccgatt
      421 ctgccgtgat tggccgcgta acgaacggag accatgagaa atggcataca gcggaaatgc
      481 gcagacttct cgccagcccg caattgcgag agtgcatcgc aggtgtcctg gtccaattcg
      541 cagagcgcga tcatctcaag ggattgaata ttgatatcga gactcttggc actgctgggc
      601 gtgcggacta tgtaaagttt ctaaaggaac tggcggatcg tttgcatgaa cgcgatatga
      661 tcctcacggt agacatcaca gcacacgatc ctgcttatga tgtaaaagca atcgctcagg
      721 tggccgacgg cgttgtattg atggcatatg atgaacacta tacgagtggt ccggctggtc
      781 cgattgcaag tcaggaatgg tttgaggaaa acgttgcgtt cactttgaaa gacgttccac
      841 cagagaaatt aatcgttggg attggaacct atgcgattga ctggtcgcaa aatcactcct
      901 acagcatgaa gttttcagaa gtaatggctc tggcagacga tatgggagca cttcctggaa
      961 tggacccgga aagtcggaac atgcattttt cttacagcga agatgggaac aatcacacgg
     1021 tctggttctt gaataacgtc accgcctgga accagaagct tgcgatcgat cgcctgggtg
     1081 tggcaggaat tggtgtttgg cgtctgggtg cggaagatcc atccatatgg aagatcttgc
     1141 cgcagcagaa acccaacatc gaagctgtag tcgagccaga agcacttcga agtgttctct
     1201 atttgagttt tggagaagtc tttcatcttc ataccgtgcc atcgcgcggt aaaatgagtg
     1261 tagagcagga tggagaagga tttatcgacg ctgcaaaata tgactccctt ccggaaggat
     1321 atttgattga gcgaatcggg aattcaattc cagacaaatc gcttgttctg actttcgacg
     1381 atggtcccga tccagtttgg actccaaaag ttctagatgc actcaaggaa agcaaagtgc
     1441 acgcgacgtt ctttgtggtg ggagagcagg cagaacgtta tcctgaaatt gtacagcgga
     1501 tcctcgcgga aggtcatgcg atcggcaatc atacctactt tcatcctgat ctcagcaaga
     1561 cttctcccga acgcgttcgc ctggagctca acagcacgca gcggattatt gaaagagaaa
     1621 ctggctacaa gaccatgctg tttcgcgcac catacaacac ggattcaaat ccagacgaag
     1681 ccccggaaat cgatccgctt cgcacggctg cggacatggg atacttgatc atcggggcaa
     1741 acatcgacgt cgccgactgg caaaaaccaa ctgctcgctt aatggcagat cgtctgattg
     1801 agcaagcgaa gaatccaaga aatcatatca ttgtgatgca tgacggaggt ggcgatcgca
     1861 gtcgcactgt ggaagctctt cgcattggca ttccggagct caaaagcaag ggatatgtat
     1921 tccaatctct ggaagacgca acagggatta gcaaatccac tttgtttcct gcgatgcctg
     1981 caagcgaggc catgcttgta tatatcaccg ccgcttcgtg gctgagcata cgctggggat
     2041 gggtgatcat tacatggctg ttccttttta caactttact tgcgatcttt cgcattgtgt
     2101 ttctcgggat tcttgtcttc aaatctgttc gcaaagcaga ggaaagaaag gacgacggaa
     2161 catttttgcc tcctgtgagc gtgctcattc cttcgtataa cgaagcgaag gtgctttcgc
     2221 agaccattca atctctgcag caaagtacgc accctcagtt cgaaatgctg gtgattgatg
     2281 atggaagtac ggacaatacg aaagaaatcg tgcgaggctt gatgaaaaaa gacaagcgca
     2341 ttcgtctggt ttcgcaacca aacgggggca aatcttccgc gctaaatcta ggaatgaaag
     2401 aggcaaaaca cgaaatcgta gtaacaattg acgcggatac catcgtcatg cctcagactc
     2461 tgacggagct ggcagcaccg ttcaaggatc cactcgtaga cgccgtatgc gggaatgtgg
     2521 aagtcggaaa cgtgcacaac gttctgacgg gttttcaatc tctagaatac atcacctctc
     2581 agaatttcga cagacgtgcc tttgacgcac tcaactgcat cagtgtggtt cctggggcta
     2641 caggcggatg gcgtcggtcc aaagttcttg aggccggtgg ctacaagagc gatactctca
     2701 cggaagacgc agatctcact cttaccatgc ttgagaatgg agcgcgcatt gtatacgctg
     2761 cggaagctcg ttcgcgcaca gaagctcctg ctaccattcg cactctttcc aagcaaagat
     2821 tccgctggag ctttggaacc ttccagtgtc tgtggaagca caggcatgca ttcttcaagg
     2881 gcccgctcgg ctgggttgca ttgcccaaca tgtttctgtt ccaggttctt ttcccggctc
     2941 tctcaccgat aggcgacgtt gtgttcattc tctccattct gcggggggac atggaggcaa
     3001 tcgctgtggg ttacctgcta ttcttgttta tggatgtctg cggttcactg cttgccttca
     3061 ttctggagaa caagcggaag cgtctgatgt tcctcgtgtt gattcagaga ttcttctaca
     3121 gacagttcat gtacatcatt acctttaagg caatgttcgc tatgctcaaa ggagcgcgat
     3181 acggatggaa caagctggag cgtcagggca ctgtcagaat gaatccagag ctggccaaat
     3241 cacactagtg ggccggcaag ttccctgata agaactttgc gcggtaatcc tgaatgtatc
     3301 gcactactgc tccgggtttg aggtcgcttt tgggcggccc gcccggcgcg tcttcttcgt
     3361 ctgcattttt gttgaacatg acgtaaggca gccacttgcg attcttcagg accatcacat
     3421 ctccctgatc cgtgatggcc cctacgatag acgacgttgt agtccccatg aagactgcga
     3481 actttctgga cttcagactg gacggtttgc ccgatgattc ttcgaggaag atatctcgaa
     3541 agtcgatttg agaatggagc tccggatgaa gctgcttctt aaacgaagaa tgatagaaca
     3601 gcaaggggat tctgtatgtg gaaagccagg actcatagcc gtgatcgcga atcccatgat
     3661 ccgacgtaac tacaatgaga gtatggtcca gctcgtgggc ttttcgcagg cctgatatca
     3721 gatcgtctat gaattcagag taatattgca ccgctgtttt tttctgaatg tcaatgtact
     3781 ctgacgcatg gccgaagatg aattcgtaga agagaaatac gcgatcgtgt tttactacat
     3841 cgttcaccag tcgcgaaacc atgattttgt cctcgcaact gtgttcgaat tcgtacggat
     3901 tgatgcagag gtatttggat ctggcctggt ccacttcttc atgcgttaga gtgtatatct
     3961 ggtcccatgg caagtctgtc acaaccagtt ccctttcggt ttctgatgcg gaatacacgg
     4021 ttcgatagtt acgattcttt acaatattaa gaagagaagg ttttccagag acaaaagaat
     4081 attggtccac atcttcatct gagtatgcct caaacgggat cagtcttcct cccaggagag
     4141 caagcatccc ggtgcgactg tccatatctg cggcaaagaa attgttgtat tcgtgagagt
     4201 gctcctcttc tcgattgaag aaggtgccgt gtggaagggc acgcgagtcc tctcgtaact
     4261 gtgcaagagt aatctgttcc atcaccagca cgatgatctt ttgaaaccgc ggttcgaagc
     4321 gcggtgcggg gtgagtccat gagtccaggt cgtccagaga gaattcagtg ggagccggat
     4381 ctactgtgag cccgggagaa cgcgcagcct ccgagacgtc ctggatcaaa tgaagaatgg
     4441 gtgaagcatt ctcattcgcc gtagcctttg caaccatgaa gaggcttccg cataatagga
     4501 aagcgagggt ggttggaata tgccagtcat ctctgagata gctcaccaca tagaccagaa
     4561 gcagggctgc gagaagcaaa atgtttcccc agaaaggaag cacattggta aagaagtaag
     4621 caatgccaga taacgttaaa tcgaagatgg aatactgtct aatggttgct tctcgaaaaa
     4681 agtagcagta ggaaaaagtt agaacgaagt aaagaagata cgcggagtga aagaacagcc
     4741 gggaaacaat tcggccgcct ggaatggcct gcaaaatccg agcaatcaga agaagagtca
     4801 ggaagacagc cgagtccgca agccacgaag agaagagagg aagcccttcc ccttcaggca
     4861 gcagaatgag tcggaagaat gcaaagaaaa caagggctcc aaacgtgagt cgagagcgaa
     4921 agcgcgcggc tcgggttgcc agtgccaggg atgtgttata catttaaagc gatctaggac
     4981 ggatgccgta tggcggtcaa tctctaggtg aactatcgtc cggaatcaga ggctctgcag
     5041 attcaatgta cttcttattt tccaggtctc tcagtgcctc tacgaacgga tgaagcaggt
     5101 tctctcttct tcgcggaggc ggtcttttgt aagccatggc cagacgcagg ttgtagccgg
     5161 gcttttcttt tgcaaaaaag tccagcagtc gtccatagta gtgagaggag gagtggaccg
     5221 gttcgtaaag caggatgaga atttctcgct ttgcattgtc ccgcagaatg cccctgtagc
     5281 gaacgatctg gtccgcgagc agagatgaga cgacgcgaga aacaaagaaa gcaaatagag
     5341 tctttcgttc atcttcacgc atggaggcca gatgtgcctc gagctgttcg gcgcgtagat
     5401 gcggcaacgc tccgttcaaa tcccgtgcga tctctgattt ttcgtgagtg agcaatgtcg
     5461 catatcgttt ctcaagagcg ggaagcccgg cgggatggag gagcatattc ttgagatctg
     5521 ccgcctggta atctgtaagg ggtgttagaa a
//
LOCUS       DAOQJL010000222         5544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8479_length_5544_cov_4.145382, whole genome shotgun sequence.
ACCESSION   DAOQJL010000222 DAOQJL010000000
VERSION     DAOQJL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8479_length_5544_cov_4.145382"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2490
                     /locus_tag="PLG78_10625"
     CDS             <1..2490
                     /locus_tag="PLG78_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23616.1"
                     /translation="ITAISREHKELPAGLLETPMKQWNVRSMGEAPTADQFGNLPILY
                     RGGAPMFDTLIRIRDVARVEDGLDEIRRISRFNGTSAVGIGIVKQRGTNAIDVAKAVK
                     AKAAEIQKTLPPGYKIALAFDSTRFIEESIGDLELTLFLSALLTSLVCFFFLGNWSST
                     INILLAIPTSIMGTFIVLYIMGFTLNTFTLLALSLAIGIVVDDAIMVLENIVRHREMG
                     ESLVQGALNGAKEISFAAVATTLSIVAIFLPVVFMKGIIGKYFFQFGIAITAAVLLSL
                     LEALTLTPMRCSQFLKVGHGGALTQLVDSWFSRLSNAYSRILEWCLNNRLKVVLASIL
                     FFAGSFLALFPIKKEMVPAQDQSLFLVRAQTPVSASMEYTDKSARAMEAWVGSRPEIA
                     RYFSAIGGFQGGQSNLVNIFVTMKEMPDRPVDPEKKKKLSQQEFSEVVRKGLSAISPD
                     LRVTVQDLSMRGFSATRGFPVEISITGSDWEVLTDSSEKIMELMKTSGQFRDVDSNYR
                     RGQREVQVYPDREKAARFGVSISTIGSVLGAMIGGVKAGQFSEGGHRYDIRVRVDTPD
                     RQQIQDIRRIFVRNNRGELVSLADVTRVEDRPALQSITRINRERSIIVTANPAPGVGQ
                     FAAVEKGIAIARGALPAGYNAEATGQSKTAGESNWELVIALLLGIVVAYMILASQFNS
                     FLHPVTVLVALPFSFSGAVLTLFAFGQSINIYSFIALLLLMGLVKKNSIMLVDFTNRI
                     RTTGQDVRSALLEACPIRLRPILMTSIATIAAAIPAALSFGPGGETRIPMALAVIGGT
                     IVSTVLTLVVVPCVYSLFRRFERMPPESLGH"
     gene            2541..3965
                     /locus_tag="PLG78_10630"
     CDS             2541..3965
                     /locus_tag="PLG78_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNE23617.1"
                     /translation="MRLVVSVFALFVGLISCSAQRPPVEGLLLEALLAQGVWQTIYPG
                     PGSFTMALRSTSGTYARSYNPICSGRPGTNPSYAFLVKRGSSSNLLINFMGGGACWSG
                     ANCLGPSSTITYYPEVASLIPATLAVGNQVIDQGILNHNRSDNPFQTWNMVFVSYCSG
                     DIHVGSADTVYADPQTGVQSVIHHHGVDNTLSVLSYLQAQFPESSVSRVFITGQSAGG
                     YGAIFNYPLIKEMYYSRQVDVLGDAANGVLDVSDTGAAANQFQQTSSVNWNATASVPS
                     WIPTINGQYLTLSIGDFYNRVATYYESGATNPAPGKSRFAQYTAAYDGNQRFFFGVMR
                     NSLKTPAKTYTTTDNMWGRTDGYDAQRLGSNPALNVSCNWNTQMRAQGTLAATSPLYK
                     RVVAAGSVHTISMSNSYYTTVIQTAGGNVRLVDWYNSMLTGSGFADAICAHAGSKCAA
                     PNTEESTPLNCTGQPGFNGAGDGW"
     gene            complement(3969..4928)
                     /locus_tag="PLG78_10635"
     CDS             complement(3969..4928)
                     /locus_tag="PLG78_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23618.1"
                     /translation="MIRILLLLLGMSAALSADTLRLGVSVDDTHLHSELQPHVKNDDL
                     FPGIGLGRIQLDQEWDHDSTFIFPLSIEYLKKLGPGSVRIGANYPRYQPHLRFDSIGS
                     LYLARTRLKEFEITDAEGQLGYDFALFSEKLTLAPWIGAHFHGTRYTTFETTLGPAQA
                     TTTIASPFQDGATGMVYGIGAQFQFLPDWAVFGSLEKDGPRRGIVGAMRDQKVTIGTQ
                     NSSPFIQIDQSNAGTLINLRKLALGVEYSISQKMRLRVGVAEDLLLTRHPGYQTVPVL
                     IVGGLATFPVYTSELLTDSVLWDKAEKQRQFHFFTSVTYGLEF"
     gene            complement(4934..>5544)
                     /locus_tag="PLG78_10640"
     CDS             complement(4934..>5544)
                     /locus_tag="PLG78_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNE23619.1"
                     /translation="EPNSAGYSGAEVARRVLDASGLSNVKIERVEGELTDHYDPSSKT
                     LRLSDSTFDSTSVAAAGVAAHEAGHAIQDKVKYPMLAFRTSIVGMAGFGSNLSWILIM
                     IGFGMMSFLRSPLGYYIALAGVAFFGVVVVFQLITVPVEIDASNRAKQVLQSMAITRT
                     GSGTGVNEVLNAAAWTYVAAAGTAVATLLYYALQLAGFRNRD"
BASE COUNT         1305 a         1412 c         1447 g         1380 t
ORIGIN      
        1 atcactgcga tcagccgcga acacaaagaa ctacctgcgg gcttgcttga gactcccatg
       61 aagcaatgga acgtgcgttc catgggggaa gctccaacag cggatcagtt tggaaatctg
      121 ccaattctgt accgcggtgg cgcgcccatg tttgatacat tgattcgcat tcgcgacgtg
      181 gcgcgcgtgg aagatgggct cgatgaaatc agacgtattt ccagattcaa tggaacatct
      241 gcggttggaa ttggaattgt aaagcagcgc ggaaccaatg caatcgatgt tgcaaaagca
      301 gtaaaagcga aagccgctga aatacaaaag actctgcctc ccggttacaa gatcgcactt
      361 gcatttgatt ccactcgatt catagaagaa tcaattggag atctggaact cactctcttt
      421 ctatccgctc tgctgaccag tctagtctgt tttttcttcc tgggcaactg gagctctacg
      481 atcaacattc ttctggccat ccccacttcg atcatgggaa cattcattgt tctctacatc
      541 atggggttta ctctcaatac gttcacactc ctggctcttt ctcttgcaat cggtattgta
      601 gtggatgatg cgatcatggt tctcgagaat attgtgcgac acagggaaat gggagagtct
      661 ctggtgcaag gggctctgaa cggcgcgaag gagatttcat tcgccgccgt ggctaccact
      721 ctgtccattg tcgcgatttt tctgcccgtc gttttcatga aaggaatcat tggtaagtac
      781 ttctttcaat ttggaatcgc aatcacagcc gccgtacttc tgtctttgct cgaagctctc
      841 actctcactc ccatgagatg ctctcagttc ttgaaagttg ggcatggtgg agcgttgact
      901 caacttgtgg attcctggtt ttcgcggctc agtaacgctt actcacgcat actcgaatgg
      961 tgcttgaaca atcggttgaa agtagtactc gcttcgatcc tgtttttcgc cggaagcttc
     1021 cttgcacttt ttccaatcaa aaaagagatg gtgccggccc aggaccagag tcttttcctg
     1081 gttcgcgcgc agactccagt gtctgcgtcc atggagtaca cagataagtc cgcgcgtgcc
     1141 atggaagcct gggttggttc gcgtccagag attgcgagat acttcagtgc cattggtgga
     1201 ttccagggcg ggcagtccaa cctggtgaac atatttgtca caatgaaaga gatgccggat
     1261 cgacccgtgg acccggagaa aaaaaagaaa ctcagtcagc aggaattttc tgaagtagtg
     1321 cgaaaaggtc tctctgcaat ttctccggat cttcgcgtaa cggttcagga tttgtctatg
     1381 cgcggatttt ctgccactcg cggttttcca gtagaaatca gcatcacggg cagtgactgg
     1441 gaagtcctca ctgattcttc tgaaaagatc atggagttga tgaaaacttc gggtcagttc
     1501 agggacgtgg attcaaacta cagaagaggg cagagagaag ttcaggtcta tccggatcgc
     1561 gagaaggcgg cgcgatttgg agtgagcatt tcaacgatcg gcagtgtact cggggctatg
     1621 attggaggcg tgaaggcagg tcagttttct gaaggcggcc acagatacga cattcgcgtg
     1681 cgcgtagaca caccggatag acagcagatc caggacatac gacgcatctt tgtgcgaaac
     1741 aatcgcggag agcttgtgag tctggcagat gtgactcgag tagaagacag accggcgctg
     1801 cagagcatca caagaatcaa tcgcgagcga tccatcattg ttaccgcaaa tccggcaccc
     1861 ggtgtgggac agtttgcggc ggtggaaaag ggaattgcga ttgcgcgggg tgcactgccc
     1921 gccggttaca acgcggaagc aaccgggcaa tcaaaaactg caggcgaatc gaactgggag
     1981 ctggtcatcg cacttcttct tggaattgtt gtggcgtaca tgattcttgc cagtcagttc
     2041 aatagtttcc ttcatcccgt gactgttctt gtggcgctgc ctttcagctt ttctggagct
     2101 gttttaacgt tattcgcctt tggccagtcg atcaatattt acagcttcat tgctctgctt
     2161 ctcctgatgg gtctggtaaa aaagaattcg atcatgcttg tggattttac aaacaggatt
     2221 agaaccacag gccaggatgt gaggtccgct cttctggagg cctgtcccat tcgtcttcgc
     2281 ccgattctca tgacttccat tgctacgatt gccgccgcca ttccggcggc gctttccttt
     2341 ggaccgggtg gagagaccag gattccaatg gctctggcag tcataggcgg gaccattgtg
     2401 tccacggttt tgactctggt agtagtccct tgtgtctata gcctgtttcg cagatttgaa
     2461 aggatgcctc cggagagtct ggggcactga atcaaaaaaa gctggcactt cgcgcacttt
     2521 ccggtttctc tggattcact atgcgcctgg ttgtctccgt ctttgcgttg tttgtcgggc
     2581 tcatttcatg ctcggcacaa aggcctcctg tggaaggcct gcttcttgaa gctttgcttg
     2641 cccagggtgt ctggcagacc atttaccccg ggccaggttc gttcacaatg gccttgcgct
     2701 caaccagcgg tacgtacgcc agaagctaca atcccatatg ctctggaagg cccggaacca
     2761 atccatcgta cgcgtttctt gtgaagcgtg gaagttcaag caatctactg atcaacttca
     2821 tgggaggagg agcctgctgg agcggagcaa attgccttgg tccttcgagt accatcacgt
     2881 actatcctga agtggcatcg ctcatacctg ccacactggc cgttggcaat caggtcattg
     2941 atcagggaat tctcaatcac aatcgatcag acaatccttt ccagacctgg aacatggttt
     3001 tcgtttctta ctgcagcgga gacatccacg ttggttccgc agataccgtg tatgcggatc
     3061 cgcagactgg agtgcaatcc gtgatccatc atcatggcgt ggacaatact ctttctgttc
     3121 tttcctatct acaggctcaa tttcctgaat cttccgtttc gcgcgtgttc attaccggac
     3181 agagtgcagg cgggtatggt gccattttca attatccttt gatcaaagaa atgtattact
     3241 ccagacaggt agatgttctg ggagacgcgg ccaacggcgt tctggatgta tcggacacag
     3301 gtgccgcggc caatcagttt cagcaaacgt cttctgtgaa ttggaatgca accgcaagtg
     3361 tgccttcgtg gattccaacg atcaatggcc agtacttgac tctttccatt ggggactttt
     3421 acaatcgcgt agccacctat tacgaatcag gggccacgaa tccggcaccg ggcaaatcca
     3481 gattcgcaca gtacacggcc gcctacgatg gaaaccagcg cttcttcttt ggcgttatgc
     3541 gcaatagctt gaagactcct gcgaaaacat atactaccac agacaacatg tggggaagaa
     3601 cagacggata cgatgcgcaa cgtctgggat ccaatcctgc actgaacgtt agctgcaatt
     3661 ggaatactca gatgcgtgcc cagggaactc tggccgctac atctcccctt tataagagag
     3721 tggttgcggc aggaagcgtt cacactattt ccatgagcaa ttcttactac actactgtca
     3781 ttcagacggc cggcggcaac gtgcgactgg tggactggta taattccatg cttaccggga
     3841 gcggatttgc agatgcgatt tgcgcccatg ccggctcgaa gtgtgctgct cccaatacgg
     3901 aagagtctac tcctctcaat tgcacgggtc agcctggttt caacggcgcc ggagacggct
     3961 ggtaattctt agaattccag gccgtacgta acgctcgtaa agaaatgaaa ctgtctttgc
     4021 ttctctgctt tgtcccagag cacgctgtct gttagaagtt ccgaagtgta gactggaaat
     4081 gtggccaggc ctcccacaat caaaacaggc acggtctggt aacctggatg tcgggtcaaa
     4141 agcaaatctt ctgcaactcc cacgcgcagg cgcatctttt gagagatgct gtactctact
     4201 cccagcgcga gcttcctgag attgatcagt gtcccggcat tggactggtc aatctggata
     4261 aacggagagg agttctgcgt accaattgta accttctggt cccgcatggc tccaacgatt
     4321 ccgcgacgcg ggccgtcctt ctctaaacta ccgaacaccg cccagtcagg cagaaattga
     4381 aactgtgcac caatgccgta taccatacct gtggccccgt cctggaaggg agaagcgatt
     4441 gtagtcgtgg cctgggccgg tcccagagtt gtttcgaatg tcgtataacg ggttccatga
     4501 aagtgagcac caatccacgg ggctagagtc agcttttcgc taaacaatgc gaagtcgtag
     4561 ccgagctgtc cttctgcatc cgtgatttca aattctttga gtctggtgcg tgcaagatag
     4621 agagatccaa tcgaatcaaa acgaaggtgc ggctggtatc tcggataatt ggcgccaata
     4681 cgaacagagc caggcccgag cttcttgaga tactctatgc tcagtggaaa gatgaatgtg
     4741 gagtcgtggt cccactcctg atcgagctgg attctaccca gtccaattcc ggggaacagg
     4801 tcatcatttt tcacgtgagg ctgcaattca gaatgcagat gagtgtcatc cacagacacg
     4861 ccaagacgaa gggtgtccgc ggacaaagcc gcggacattc caagaagaag taggagaatg
     4921 cgaatcacag cgactagtct ctgttacgaa atccagccag ctgcaaggcg tagtagagga
     4981 gagtggccac tgcagttccg gcagccgcca catacgtcca cgccgccgcg ttgagcactt
     5041 cattgactcc tgttccactt ccagtgcggg tgatggccat actttgaagc acttgcttcg
     5101 cgcgattgga agcgtcgatt tcaacgggca cagtgatcag ttgaaacaca accaccacgc
     5161 caaagaaagc aacacccgcc agagcgatgt agtagcccag aggagagcga agaaatgaca
     5221 tcatcccaaa accaatcatg atgagtatcc agctcaggtt ggagccgaat cctgccatgc
     5281 ccacaatgga tgtgcgaaag gcgagcattg gatatttcac tttgtcctga attgcatgac
     5341 cggcttcatg agcggccact cccgccgcgg ccacgcttgt ggaatcgaac gtggaatcag
     5401 acaggcgaag agtcttggaa gatggatcat aatgatccgt aagttcgcct tccactcttt
     5461 cgatttttac gttgctgagg ccggacgcgt ccagcactct gcgagcgact tccgccccgc
     5521 tgtagccagc cgagtttggc tcct
//
LOCUS       DAOQJL010000223         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8497_length_5536_cov_5.173326, whole genome shotgun sequence.
ACCESSION   DAOQJL010000223 DAOQJL010000000
VERSION     DAOQJL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8497_length_5536_cov_5.173326"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLG78_10645"
     CDS             <1..251
                     /locus_tag="PLG78_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNE23620.1"
                     /translation="EKPGILDIAAHPSAIAFLGHMRSSENLSPSEIFRLNTESRVAER
                     IYSNDGKQISGSSTGLVWNGMLIVGQVFEPFLLTCNAD"
     gene            complement(231..1946)
                     /locus_tag="PLG78_10650"
     CDS             complement(231..1946)
                     /locus_tag="PLG78_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE23621.1"
                     /translation="MSQFSDSLCAQQMRMLRLDLPVSSEAEVARLHSKYDPWEEARRQ
                     LGQIESRPVVLAFGAGLGYAIALLLRSGKECIWFESDPRILSRALGTVDLQEFIQSGR
                     LRVVRRISNEEELEEIFRGRGNDDISFFSHRNFLTETHAEWKRKIEFFLNKKSVNMAT
                     LSRFESIWARNLVANMPLLLRGQPVSTLFGIHSNKPAIVVGAGPSLSDSIGDIEKVRQ
                     KAVLICVDTALAVLRHEGIDPDYVVTVDPQPVNRCYLEGYDGEALIVADPTASRFALR
                     HVVADRIRYFWSPFAMAQVFFEAIGVTAGEIAFGGSVSTNAYDLARKMGCDPVFVVGQ
                     DLSFPGGEVHCRGATLEERLNWKESKTFRRELHNYRQLSALPPVLLPGLTQASERTND
                     KLVIFYRWFERRFRKDLENGLRIRNCTARGARFDFLERAQLSELAGLPDAMPGPQMIR
                     ANDVSDKARKLLLVLESLEADFASTSKELELAVQIIRKEGPPKKTGETLDRVDEELRK
                     RKNALAILGNASQKAIHAVTESAHGGERALELYEALYSASLRYERWLKRSIRILSETL
                     VGIAR"
     gene            complement(1943..2707)
                     /locus_tag="PLG78_10655"
     CDS             complement(1943..2707)
                     /locus_tag="PLG78_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE23622.1"
                     /translation="MIVAHNVRYVRQGNTILDGITWKAERNEHWIMLGPNGSGKSSFI
                     KLLTLYEWPTDGFIEIDGMRSGTEPVSVMRRKIGLFEPALHSSIAHFYPDMTGEEIVQ
                     AGARGALALYESPPEEVVHRARKLLVEERLPWERPFSVMSSGEQRRTLLMRALMPKPS
                     MLILDEPFESLDMKARYQLESTLKRNTEGMVTLTALHRIEEIPAHASHALLMKNGKVL
                     RSGPLEELLNRENLSELYEMQLEVTKHAGRYSCRPT"
     gene            complement(2704..4227)
                     /locus_tag="PLG78_10660"
     CDS             complement(2704..4227)
                     /locus_tag="PLG78_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE23623.1"
                     /translation="MDGSSKHTLPVKVVTRIEPGQEDQRGIQGILNGAGLGFRYSGLI
                     DARFNFVSGFLHRLLCEVDLGYRTELVLTILRELATNCSKAHAKRIYFQSQGLDISIP
                     ADYASGMASFRADVLSHWDQFVAANSTNEFYIELSFELNADAIVIHMENNVELLPSEW
                     ERITQRRQKAAQIRDILEAFNAFDDDNEGAGLGIVFISLLLRNAGIAESNLIIDSRRG
                     VTRHTLIVPRLPAQLRSGIRDEMLREIEGLPSLPDRVNQLVVLCSDSSASIASIAQEI
                     ERDPAMAAQILRLVHSAGFMTRRRNMDVESAVKVIGLQEVSELVVATASRSVLANHFQ
                     IKEMETIWDASNRVSFFAAELAERCHVKRSDAAIAGLLLELGKIVMIGLKQTTMERID
                     EMLGSKRVRTSSILEESTLGISHPEIGARLGEKWNFPQAVIDCIRYQHKPLLCVSPNE
                     ALVRAVSLASRLHASTQGMADYYAVDPEVLQAGRVHSPEEFAKLAKLLEEQFKKQIA"
     gene            4260..4556
                     /locus_tag="PLG78_10665"
     CDS             4260..4556
                     /locus_tag="PLG78_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23624.1"
                     /translation="MNRQWQEFKKAPGLYFRFINGKLELLGKYDRDPSQWAQIAGEIK
                     AQWEIENLREKPALDEGLQGNLFSDEDHELAGKLRTLPLLRPEKPLRPHKRRSA"
     gene            complement(4567..>5536)
                     /locus_tag="PLG78_10670"
     CDS             complement(4567..>5536)
                     /locus_tag="PLG78_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23625.1"
                     /translation="PEDHMSVPKSIIAAMFFMGGVAAIISQTPPATTPATTPVAAETK
                     PAGPASAIQLQDFDPVAVKDPRFTLTNVNFDRRYAPNGVGEFLDVVFDLKNLTSEPVD
                     FYAYVIAFWETDAVDLNSRRLIPYPTWRVNDPARGEYLVHYITITPKDLPDEEIWSDK
                     DPDYKHYSHIVQSMRDSVATTEPVSDVHPPYWKYLSYISQHPAQGLKFKLYGDKGPGQ
                     HEVIQTNYVPPTAEEKKNKVHRTLSQHKFTIEHTRRMAIFRSHHYTAYRADFKFFNSV
                     SIVLFDAKKADAYEEQRKAGGPKAGEAKIDPLIYKQTFKIGRPLKM"
BASE COUNT         1348 a         1516 c         1309 g         1363 t
ORIGIN      
        1 gggaaaaacc tggtatcctg gatatagcgg cgcatccttc tgcgatcgct ttcctcggac
       61 atatgcgatc ctcagagaac ctttcccctt cagaaatctt caggctgaac accgagagcc
      121 gggttgccga acgcatctat tctaacgatg gaaaacagat cagcggatct tccacgggtc
      181 tcgtgtggaa cggaatgctg attgtcggcc aggtctttga accgttcttg ctaacgtgca
      241 atgccgacta acgtttccga aaggatccgt atggatctct taagccaacg ttcgtacctg
      301 agcgacgctg agtaaagagc ctcatacaac tccagtgctc gctcgcctcc gtgtgcagat
      361 tcagtcacag cgtgaatcgc cttctgcgac gcatttccca gaatcgccag ggcattcttt
      421 ctcttgcgca gctcttcatc gacgcgatcc agagtctccc cggttttctt cggcggtcct
      481 tccttccgaa tgatctgcac cgcgagctcg agctcttttg atgtggatgc gaaatccgct
      541 tctagcgatt cgagaaccag aagcaatttg cgagctttat ccgaaacgtc attcgcgcga
      601 atcatctgtg gaccgggcat tgcgtccggt aaaccggcaa gctcgctgag ctgagctctc
      661 tcaagaaaat caaaacgagc accgcgagca gtgcaattgc gaattcgcag accattttcg
      721 aggtcctttc gaaaccgcct ttcaaaccat cgatagaaaa tcacaagctt gtcgttggtt
      781 ctctcgcttg cctgggtgag tccaggcagc aacactggag gcagggcgga aagctgtctg
      841 tagttgtgaa gctcacgcct gaacgtctta ctttccttcc agttcaatct ctcttcgaga
      901 gtcgctcctc tgcagtgcac ttctccacca ggaaagctca gatcctgacc gacgacgaag
      961 accggatcgc atcccatctt cctggccaga tcatacgcat tcgtcgaaac agatcccccg
     1021 aatgcgattt caccagcggt cactcctatg gcctcgaaga atacttgtgc cattgcgaag
     1081 ggagaccaga agtagcgaat ccgatccgcc accacatgac gcaatgcaaa tctcgaggca
     1141 gtgggatcgg ccacgatcaa cgcctcgccg tcgtatccct ctaaatagca acgattcacc
     1201 ggttgaggat ctacagtcac cacatagtca gggtcaatac cctcatgccg aagcacggcc
     1261 agagctgtgt ccacgcaaat taagaccgct ttctggcgaa ccttttcgat atcgcctata
     1321 ctatccgaga gggacgggcc cgcacccact actatcgcgg gcttgttcga atgaatgccg
     1381 aataacgtag aaacaggttg tcctcttaga agaagaggca tattggctac gagatttctt
     1441 gcccagatgc tctcgaaccg ggatagcgtt gccatgttca cactcttctt attcaagaag
     1501 aattcgattt ttcgcttcca ttctgcatgt gtttctgtta gaaaatttct atgtgagaaa
     1561 aacgagatgt catcgttccc tcgaccccgg aagatctcct ccagttcttc ttcattagag
     1621 attcgtctaa ctacccgaag gcgaccagac tggatgaact cctgcaaatc gacagtaccc
     1681 agtgcccgac tcagaatgcg cggatcgctt tcaaaccaga tacactcctt tcccgatcgc
     1741 aagagcagcg caatcgcata gcccagccca gccccaaacg cgagaacgac cggacgcgat
     1801 tctatctgcc ccagttgtcg ccgcgcctct tcccatggat cgtacttgct gtgaagtctc
     1861 gctacctccg cctcagaaga aactggtaga tcgagacgca acatgcgcat ctgttgcgcg
     1921 cacaatgaat cagaaaactg gctcatgttg gtctgcatga gtatcgaccc gcatgctttg
     1981 taacctctaa ttgcatttca tacagctcag aaagattctc gcgattcaac agctcttcga
     2041 ggggcccgga tcggagcacc ttgccgttct tcatcaacaa agcatgactc gcatgcgctg
     2101 gtatctcttc gatgcgatga agcgcggtga gcgtcaccat cccctctgtg tttcgcttca
     2161 aagtggattc aagttgatag cgcgccttca tatcaagcga ttcaaaaggt tcatccaaaa
     2221 tcaacatgga gggctttggc atcagggcgc gcatcaacag agttcgccgc tgctctccac
     2281 tcgacatgac actgaatggc ctttcccagg gtaggcgctc ctccaccaga agcttcctcg
     2341 cacgatgaac cacttcttcc ggtggtgatt cataaagggc gagagctccc cgcgcccctg
     2401 cttgaacgat ttcttcgcct gtcatatccg gataaaaatg agcgatcgat gaatgcagtg
     2461 caggttcaaa caggccgatt ttccgccgca tgacgctcac tggctccgta ccacttcgca
     2521 tcccatcgat ttctatgaat ccatcggtag gccattcata caatgtcagc agcttaatga
     2581 aggaagattt tccagatcca tttggaccga gcatgatcca gtgctcgttt ctttccgcct
     2641 tccaagtgat cccatccagg atcgtgtttc cctggcgcac atagcgcacg ttatgcgcaa
     2701 cgatcatgct atttgttttt taaattgctc ttcaagtaac tttgcaagct tcgcgaactc
     2761 ttccggggaa tgcactctac ccgcctgcaa tacctcagga tcaacggcat agtagtctgc
     2821 catcccctga gtgcttgcat gcaatctaga ggcaagcgaa accgcgcgca cgagagcctc
     2881 attcggagaa acacagagca agggtttatg ctgatatcga atgcaatcta tcacagcctg
     2941 cggaaaattc catttttctc ctaaccgagc gccgatttcc ggatgcgaaa tcccaagcgt
     3001 actttcctct agaatggaac tcgttcgcac gcgtttgctg ccgagcattt cgtctatgcg
     3061 ctccattgta gtttgcttaa ggcctatcat gacgattttg ccgagctcga gcaatagacc
     3121 cgcgatggcc gcatcacttc tctttacatg acaacgttca gcaagctccg cggcgaaaaa
     3181 tgatacacga ttgctcgcat cccagatagt ttccatttct ttgatttgaa aatgatttgc
     3241 aagaacgctg cgactggcag tagcaactac cagctcagac acttcctgca ggccaatgac
     3301 cttgaccgca gattctacgt ccatgtttcg tcgcctggtc atgaaaccgg cagaatgaac
     3361 cagccgtagt atttgcgcag ccatggctgg atctctttcg atttcttgtg cgatggaagc
     3421 aatagacgcg gaagaatcac tgcataaaac gaccagctga ttgacccgat caggcaacga
     3481 cggaagccct tcaatttcgc gcagcatttc atcgcgtatt ccactacgta gttgcgcggg
     3541 caatcgcggt acgatcaaag tgtgtcgagt cacgccgcgt ctcgagtcga tgatcaaatt
     3601 ggattccgca atgccagcat tgcgcagtag taaagaaatg aaaacaattc ccaggcctgc
     3661 tccttcgttg tcgtcatcga atgcattgaa tgcttccagt atgtccctga tctgcgctgc
     3721 tttctgtctt ctttgcgtga tcctttccca ttctgaggga aggagctcta cattgttttc
     3781 catgtggatc acgatcgcgt ccgcattgag ttcaaaactc agctcaatgt aaaactcgtt
     3841 tgttgaatta gcagctacaa actgatccca gtgagaaagc acatccgccc ggaaggatgc
     3901 cattccggaa gcataatctg cagggataga aatatcaagg ccttgcgatt gaaagtagat
     3961 tcgctttgca tgtgctttcg aacaattcgt cgcaagctcc cgcaaaatgg tcaaaaccag
     4021 ctccgtgcga taaccgagat ctacttcgca gagcaacctg tgaagaaatc cagaaacgaa
     4081 attgaatcgc gcgtcaatga gcccagaata gcgaaacccg aggccagctc cattgagaat
     4141 cccctgaatg cctctttgat cttcctggcc tggctcgatg cgagtcacga ccttaactgg
     4201 cagtgtatgt ttggaactgc catccatttt tgtcacacag gcccgataga ctctggttta
     4261 tgaacagaca atggcaggag ttcaaaaagg cacccggtct atattttcgc tttatcaacg
     4321 gcaaactgga gcttctgggt aaatacgacc gcgatccgtc ccaatgggcg caaatcgcag
     4381 gggaaatcaa ggcacaatgg gaaatcgaaa atcttcggga aaagcccgcg ttagacgaag
     4441 gcctccaggg aaacttgttc tcagatgagg accatgaact ggctggaaaa ctcagaacgc
     4501 tgcctttgct ccgtcccgag aagccgctgc gaccacataa gcggcgcagc gcatgacttc
     4561 ggttaattac attttcaggg gacgaccaat cttgaacgtc tgcttgtaaa tcaaaggatc
     4621 gatttttgcc tcgcccgcct ttggaccgcc ggctttcctt tgctcttcat aagcatctgc
     4681 tttcttcgca tcgaacagca caattgaaac agaattgaag aacttgaaat ccgctctata
     4741 tgccgtgtaa tgatgcgaac ggaaaatcgc catgcgacga gtatgctcaa tcgtgaactt
     4801 atgctgcgag agagtgcgat gcactttgtt ctttttttcc tcagctgtag gcggaacgta
     4861 gtttgtttga atcacttcgt gctgtcccgg acccttgtcc ccgtaaagtt tgaacttcaa
     4921 tccttgcgcc gggtgctgcg aaatatagct cagatacttc cagtatggcg ggtgaacgtc
     4981 tgagaccggt tcggtagtgg ccactgaatc gcgcatgctt tgcacgatgt ggctgtagtg
     5041 tttgtaatcc ggatccttat cactccagat ttcttcgtct ggaaggtctt tgggcgtgat
     5101 ggtaatatag tgcaccagat actcgccgcg agccggatca ttcacgcgcc aggttggata
     5161 cggaatcagt ctgcgcgaat tgagatccac cgcatcggtt tcccagaacg caatcacata
     5221 ggcatagaaa tcgaccggtt cgctcgtgag attcttgaga tcaaacacta catcgaggaa
     5281 ctcgcctaca ccattgggag catatcttct atcaaagttc acattcgtaa gcgtgaaccg
     5341 cggatccttc acggcgacag gatcaaaatc ctgcagctgg atggcagaag ctggcccggc
     5401 cggttttgtt tccgccgcca ccggagtggt cgcgggagtt gtggctggag gggtctggct
     5461 gatgatggcg gccacacctc ccataaaaaa cattgcagca attatcgact tggggacgga
     5521 catatgatcc tctggc
//
LOCUS       DAOQJL010000224         5518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8529_length_5518_cov_3.139301, whole genome shotgun sequence.
ACCESSION   DAOQJL010000224 DAOQJL010000000
VERSION     DAOQJL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8529_length_5518_cov_3.139301"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PLG78_10675"
     CDS             <1..1749
                     /locus_tag="PLG78_10675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23626.1"
                     /translation="KWSRELPAPHIQKRMILLRAEARALTGHPRALADYSTAMQLAGQ
                     DEDILIRALAAERAGRYCRRIGAPEAADHYVSDALYYYSVYGADHKLSLLQNDFPAAQ
                     IRKTETRATESADLLAITRTAIAISSEIVQSRLIERVLESILVQAGARRIAYLHEHDG
                     RLCMAAVGQADPEIKVEVVHKLAADVPFIPQLLVHNVMNKREPVILNDASQDETFNMG
                     DLSIRSALCMPLLVKGNVAGVLYLDNDLATGVFTEEKLGFLSVLTSQFAVSLENAALY
                     SGLEKRVQERTAELENSLEQVRTLKLAQDLDYYLTALLLQPLSGIRITTDGLAIQQHV
                     LQKKKFKYMRWEEQLGGDICIARDVRIGGRICTAIVNADAMGKSMQGAGGALVFGAVF
                     ESITASSYEDSGPEQWLRNCYTELNEVFRSFSGYMMISAVISLVDTKARTIFSINADH
                     PLPVLVRDGHASFQPQTVLPRFGMRPTPPEIEINVTQANEGDIFILASDGRDDLIVPG
                     PDGPTLNEDPDLFLHTVEDNAGDAETIFKTLCAGDRLTDDLSIISVTFPAAASHNLSQ
                     QDARDASVRESEHSPH"
     gene            complement(1691..2212)
                     /locus_tag="PLG78_10680"
     CDS             complement(1691..2212)
                     /locus_tag="PLG78_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23627.1"
                     /translation="MLELVENAVKANLKRAFFSKNTYNFEDPTSYTAGIESFVKSYPD
                     IRQGHYDSALKDLELVVSVQVDYNDHRLLVYVENNAVLVSQEEIRIRKQLAAAMLSDK
                     IAEFYVQYGDETEGSGLGLAMIVVLIRNLGFDAHNFRVFQKDNRTIARLEFPLNADYV
                     PIRERWRREHPAG"
     gene            2440..4494
                     /gene="glgX"
                     /locus_tag="PLG78_10685"
     CDS             2440..4494
                     /gene="glgX"
                     /locus_tag="PLG78_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNE23628.1"
                     /translation="MKIEATLPGRSFPPGSTVLPEGVNFCVFSKNCQFIELLFFDDVN
                     AKEPSRIIPLHPENNRTFYYWHILVKGIKPGQLYGYRAQGPFEPETGLRFDGEKILID
                     PYGKAVAVGDSYDRTAAQKPGSNAHCCMKSVVVDTASYDWEGDMPIHRDLRHSFIYEL
                     HVGGFTRSMDSGIAEEKRGTFAGIIEKIPYLKSLGVTEVELLPVQHFDAQDAPLGKPN
                     YWGYSPVSFFAPHSGYATHSDPQAPVREFRDMVKALHRAGIEVILDVVFNHTAEGDHT
                     GPTLSFRGLENRAYYIPDPENRSRYANYSGCGNTFNANQSIARRMILDSLRYWVSEMH
                     VDGFRFDLASVLSRDEWGAPLRSPPILWEIESDPVLAGTRIIAEAWDASGLYQVGSFI
                     GHKWAEWNGIFRDDVRRFVRGDRGLVPKLMQRITGSPDVFPQKEWEPERSINFLTCHD
                     GFTLNDLVSYNQKHNESNGEQNRDGMDENYSWNCGEEGISSNPLVNALRKKQIKNFLT
                     LLFISQGTPMIQMGDEAARTQNGNNNAYCQDNETSWFRWKDLETNAEILRFQRELAEF
                     RKANTVFHMPRFWTSGSPEKPVLRWSGVRLWEPDTGHVSHSIAFQIQEPEKSEKIFVI
                     LNAYHGPLDFELPPSETGWKRAIDTSLPAPDDICPPSSRPVIPGKQYTANERTFIVLT
                     TG"
     gene            4496..>5518
                     /locus_tag="PLG78_10690"
     CDS             4496..>5518
                     /locus_tag="PLG78_10690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE23629.1"
                     /translation="MDTAIFSLGPATIDSTVTVPQFINDDERVLIDLQLSSVKAAFAS
                     GGPTSFENAGPRRKIFFEPSRTKAAIVTCGGLCPGLNNVIQSVVRMLYFSYGVRQILG
                     VRYGYEGLIPDFGHSFLELTPQLVHDIHKTGGTILGTSRGPQDIGRMVDTLVANKIDI
                     LFTLGGDGTLRGADAIHEEIKRRGLKISVAGIPKTIDNDIQFAHKTFGFATAVSIANE
                     SVHAAHAEAKSARNGIGLVKVMGRDSGFIASYVALASNEVDYVFVPESHFELRGEKGF
                     LPHLEKRLAESKHAVVLVAEGAGQKYCEITGEKDASGNVKYGDVGVVMSTEIKKYFKE
                     KNIPVVL"
BASE COUNT         1468 a         1429 c         1349 g         1272 t
ORIGIN      
        1 aaatggagcc gtgaacttcc cgctccccat atccagaaga gaatgatcct tcttcgcgcg
       61 gaggctagag ctctcacggg ccacccacgt gcccttgcag actacagtac agctatgcaa
      121 ctggcaggtc aagatgagga cattttgatt cgcgctctgg ccgcagaaag agccgggaga
      181 tattgcagaa ggattggcgc accggaggca gcggatcact acgtttcgga cgccctgtat
      241 tactattccg tttacggggc agaccataaa ttatccctgc ttcaaaacga ttttccggca
      301 gcacaaattc gcaaaacgga aacgcgtgcg acagaatctg cagatctgct ggccattact
      361 cgcactgcca tcgccatctc gagtgaaatc gtacagagca gactcattga aagagtactg
      421 gagtccatcc tggttcaggc gggcgcaaga cgaatcgcct acttacacga acacgacggg
      481 cgtctatgta tggcggcggt gggtcaggca gatcctgaaa tcaaagtaga agtagttcac
      541 aaactggccg cggacgttcc gttcattccc cagctcctgg ttcacaacgt gatgaacaag
      601 cgtgagcccg taattctaaa tgacgcctcc caggacgaaa cgttcaatat gggcgacctg
      661 tccattcgat ctgctctttg catgcccctc ctggtgaaag gcaatgtagc gggggtgttg
      721 tatctggaca atgaccttgc cacgggagtt ttcacggaag aaaaactagg atttctctct
      781 gtgcttacct cgcaattcgc agtctctctg gaaaatgcgg cgctttactc cggccttgaa
      841 aagagagtcc aggagcgcac ggcagaactg gaaaattcac tggaacaagt tcgaacattg
      901 aaacttgccc aggatctaga ctattatcta acagctctcc tcctgcaacc gttaagcggc
      961 attcgaatca ctacagacgg cctggccatc cagcaacacg ttttgcaaaa gaagaaattc
     1021 aaatacatgc gttgggaaga acaactggga ggagacattt gtatcgcacg ggatgtgcga
     1081 atcggtggta gaatttgcac cgcgattgta aacgcagacg ccatgggaaa atctatgcag
     1141 ggtgcgggtg gcgcactggt atttggcgca gtgtttgaat ccatcacggc atcctcgtac
     1201 gaagattcgg gacccgagca atggctgcga aattgttata cagaactgaa tgaagtgttc
     1261 cgcagcttca gcggctatat gatgatttcc gctgtaattt cactcgtgga cacaaaggca
     1321 cgaaccattt tcagtatcaa tgcagaccat cctttgcccg tactcgttcg ggacggacat
     1381 gcatcctttc agccgcaaac agtactgcct cgatttggaa tgagaccaac tccacctgaa
     1441 attgaaatca atgtaacgca agcgaacgaa ggggacatat tcattcttgc atcggacggc
     1501 cgcgacgatc tgatcgtgcc gggaccagac gggcccactc tcaatgaaga tccggatctt
     1561 tttctacata ccgtagaaga caacgccgga gatgcagaaa caattttcaa aactctctgc
     1621 gcaggggatc gcctcacaga tgatctgtct atcatctccg tgacttttcc agccgcggcg
     1681 tcacacaatc ttagccagca ggatgctcgc gacgccagcg ttcgcgaatc ggaacatagt
     1741 ccgcattgag aggaaattcg aggcgtgcga tggttctgtt gtccttctgg aatacacgaa
     1801 agttgtgagc atcaaaacca agattgcgga tcaaaaccac aatcatggcc aggccaagac
     1861 cacttccctc cgtttcgtcg ccgtactgaa cgtagaattc cgcgatcttg tctgaaagca
     1921 tggcagctgc gagttgcttg cgaatcctga tttcttcctg cgaaacaaga actgcattgt
     1981 tctccacgta caccagcaac ctgtgatcat tgtagtctac ctgaacggaa accaccagtt
     2041 ccagatcctt gagggcagaa tcatagtgac cctgtctaat atcgggatac gattttacga
     2101 aagactcaat tccagcagtg taactggttg gatcttcaaa attgtaagta ttcttgctaa
     2161 aaaacgcgcg cttcaagttg gctttgactg cattctccac gagctcgagc acagcggtgg
     2221 tcagaactac tccaatgcca tcaatctaca ggtctgcttc caggttttca aaaacggccc
     2281 gaagacgatt tcgcgcgtcc gcgtccacgt atcgaacctg cacctgttgt cgtccgaaag
     2341 ccatttgatc ccatgctcaa aacgagttgc tgatccggat agcgaaaatt cacgaaattc
     2401 acttgagccc gtcggctctt gcactcatgt gaggcgagca tgaagattga agcaacactg
     2461 cccggcagat ccttcccacc cggttccacg gtgctgcccg aaggtgtaaa tttctgcgtc
     2521 ttttcgaaga actgccaatt catcgagctt ttgttcttcg atgatgtaaa cgcaaaggaa
     2581 ccctcacgaa tcattccact ccatcctgaa aacaatcgaa ccttctacta ctggcatatt
     2641 cttgtgaaag gaatcaagcc cggtcaactc tatgggtatc gcgcccaggg gccgtttgaa
     2701 ccagaaacag gactgcgctt cgacggtgaa aagatactga tcgatccgta tggaaaggca
     2761 gtggcagtcg gtgattccta tgatcgcact gctgcccaaa agccagggag caacgctcat
     2821 tgctgcatga agagcgtggt cgtggacaca gccagctatg actgggaagg agacatgccg
     2881 atccacaggg atcttcgtca ctcgttcatt tatgaactcc atgttggtgg tttcactcga
     2941 agcatggaca gcggtatcgc agaggagaag agaggaacat tcgcaggaat cattgaaaag
     3001 attccctatt tgaaatcact cggagttaca gaagtggagc tccttccagt gcagcatttt
     3061 gatgcgcagg atgctcctct ggggaaaccc aactactggg gctacagccc tgtgtccttc
     3121 tttgcgcctc actccggtta tgcgactcat tctgatccgc aggcacctgt acgcgaattc
     3181 agagatatgg ttaaggcact ccatcgcgct ggaattgaag taattcttga tgtggttttc
     3241 aatcacacag cagaaggaga tcatacaggg cctactcttt cctttcgcgg ccttgaaaat
     3301 cgagcctact acattccaga tcctgaaaac agatccagat atgcgaacta cagtggttgc
     3361 ggaaacacat tcaatgcgaa ccagtccatt gcacgcagaa tgatcctgga cagcctgcgc
     3421 tactgggttt ctgaaatgca tgtagacgga tttcgttttg acctggcgtc cgtgctttcg
     3481 cgcgacgaat ggggagcgcc acttcgcagc ccaccgatcc tgtgggaaat cgaatcggat
     3541 cctgttctag cgggcactcg catcattgca gaggcatggg acgcatccgg gctctaccag
     3601 gttggatcgt tcatcggtca caagtgggcg gaatggaatg gaatctttcg cgatgatgtt
     3661 cgtcgatttg ttcgcgggga cagaggcctt gttccaaaac tcatgcaacg aatcacggga
     3721 agtcctgatg tatttccgca aaaagaatgg gaacccgaac gaagcatcaa cttcctcacg
     3781 tgccatgacg gattcactct caacgatctc gtttcctaca atcagaaaca caatgaaagc
     3841 aacggggaac aaaacagaga cgggatggac gagaactaca gctggaattg cggcgaagaa
     3901 ggcatatcct caaacccact ggtaaatgca ctccgcaaaa aacagatcaa gaattttctc
     3961 actctattgt tcatttcgca gggaactccc atgatccaga tgggcgacga agctgcgcga
     4021 acgcagaacg gaaataacaa cgcatactgt caggacaatg aaacgtcctg gtttcgctgg
     4081 aaagatttgg aaaccaacgc agagatcctt cgcttccagc gcgaactggc ggaatttcgt
     4141 aaggcaaaca ccgtattcca tatgccccgc ttctggactt ccggttcccc ggaaaagcca
     4201 gttctgcgat ggtccggagt ccgtctctgg gaaccggata cagggcatgt ttcgcactcg
     4261 atcgccttcc agatccagga gcccgaaaaa tctgaaaaga ttttcgtgat tctaaacgca
     4321 taccacggtc ctctcgattt tgaactgccg ccttccgaaa ctggctggaa acgcgcgata
     4381 gatacatctc ttcctgctcc agatgacatt tgtcctcctt cttcgcgccc ggttataccc
     4441 ggaaaacaat atacagcaaa cgagcgcacg ttcattgtat tgaccacagg ataatatgga
     4501 cacagcaatc tttagcctcg gccccgcaac cattgactct accgtcacag tcccccaatt
     4561 catcaacgat gatgaaagag ttctgatcga tctgcagctc agctctgtaa aggcagcctt
     4621 cgctagcgga gggccaacga gctttgaaaa tgcgggtccc cgtcgaaaaa tattcttcga
     4681 accttcgcgc acaaaagcgg ctatcgttac gtgtggaggt ttatgccctg gactgaacaa
     4741 cgtcattcaa agcgtggtga gaatgctcta cttctcttac ggagtccggc agatcctggg
     4801 cgttcgctat ggctacgaag gtctgattcc agattttggt cattcatttc tggaattgac
     4861 tccacaactc gtgcatgaca tacacaaaac cggcggcact atcctcggaa catcgagagg
     4921 tccacaggac atcggcagaa tggtggacac actcgttgca aacaaaattg atattctgtt
     4981 tacgctcggc ggcgacggca ctctacgagg cgcagacgcg attcacgaag aaatcaaaag
     5041 acgcggctta aagatctcag tcgccggaat tccaaagacc atcgacaacg acattcaatt
     5101 cgctcacaaa acatttggct tcgcaactgc cgtttccatt gctaacgagt ccgttcacgc
     5161 ggcacatgcg gaagcaaaat cagcgcgcaa tgggatcggt cttgtcaaag tgatgggtcg
     5221 cgattctggc ttcatcgcat cttacgttgc gctcgcttcc aatgaagtgg attatgtttt
     5281 cgttccggaa tcacatttcg aacttagagg cgaaaaggga ttcctccctc acctggaaaa
     5341 gagactcgca gaatccaaac acgcagtcgt ccttgttgca gaaggtgcgg gccagaagta
     5401 ttgcgaaatc acaggagaaa aggatgcatc tggaaatgtg aaatacggcg atgtgggcgt
     5461 tgtgatgagc acagagatca agaaatattt caaagaaaaa aacatccccg tagttctc
//
LOCUS       DAOQJL010000225         5511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8538_length_5511_cov_3.703446, whole genome shotgun sequence.
ACCESSION   DAOQJL010000225 DAOQJL010000000
VERSION     DAOQJL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_5511_cov_3.703446"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1178)
                     /locus_tag="PLG78_10695"
     CDS             complement(6..1178)
                     /locus_tag="PLG78_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23630.1"
                     /translation="MRRLLRYVALAFYLAGRLRRVLRNARFRSPIQSKLAIFDREGSQ
                     YLLGYCLPDVKACVLPVRGEEYLLGARVLWYMLLEVRIFFLRTRFYRDIASRSFHVYL
                     TACIRASGARVVVTHIDNNHHFRWIAEFSGAATFCAVQNGTRLPVEERWNTGEPILPL
                     PEFFCYSQFDIDIFRRYEVPVKQFHPVGSLIAGYYRKRVPWSGSSREFDVCFISDWYP
                     GVHRDFEIPELKRAILVLHYFLRRWTRNSKARVCVATRSPNPGEKEYYKRLLGEATHL
                     ADFDPVSRSTYRIMDQSLVAVTSASTAGREAFGWGQRVLFSNFSGLAQYDPPTTGPWH
                     INRADYSSFARALDDLLNMSDMDYEKQSGPARAYFMPYPDVPAHEFIRNAILTKLE"
     gene            complement(1175..1867)
                     /locus_tag="PLG78_10700"
     CDS             complement(1175..1867)
                     /locus_tag="PLG78_10700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNE23631.1"
                     /translation="MIGGKKVLALIPARGGSRGIPRKNIKILGGRPLIGWTIEAARQA
                     AFLDMIVVSSEDEEILTVAKSLGAQALKRPVELATDEASGMDPVRHAIDCFPEIEILL
                     LLQPTSPLRTSGDIDGFMQAFIASGCQSAVGLSPAPSPPEWMYRLDRDGKMKAILGGK
                     VPARRQDLEPAYVINGALYAASSAHLRAGKGFSESGAFGYVMPADRSIDIDTPLDWAM
                     AEFQLSQRPNPA"
     gene            complement(1864..2913)
                     /locus_tag="PLG78_10705"
     CDS             complement(1864..2913)
                     /locus_tag="PLG78_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE23632.1"
                     /translation="MRSWEQSILAPERSILDALKQIDATGIQLVLVAGPAGKLEGVLT
                     DGDIRRAILKGQSLDIAARDVMNSNPVTVRPDTSHEEMLSIMRRGVLHHLPVVDADRR
                     IVGLATLDDLIGAVEKPNRVVLMAGGQGKRLRPLTEDTPKPLLAVGGKPILENILENF
                     AEQGFRRFYVSVNYLAHTISDYFGDGTKWGVKIEYLHEKQSLGTAGALSLLPEVPEEP
                     ILVMNGDLLTRANFSAMLKFHEENKAAATMAVKEYDFQVPYGVVRIEGHEIRGIEEKP
                     VHKFFVNAGIYTISPAALKLIPSGQFFDMPTLFDKLRTAGQSTSAYPLREYWLDIGGL
                     EDFRRANGEFGETFS"
     gene            complement(2922..3554)
                     /locus_tag="PLG78_10710"
     CDS             complement(2922..3554)
                     /locus_tag="PLG78_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HNE23633.1"
                     /translation="MKSGLLLLIAGGGHGRVVLDSALRAGISVDGVIDGALSPGELIF
                     GIPVLGNDSYLESLPASGNSVLNGFGAVKNVERRRTQYSAWLQMGFTVQGVLHPDTIV
                     GNECEIDPSAQILAGAVIQNRVRIAENVVINTAARVDHDCRVGAHCYVSPGAILCGGV
                     TLEESVFVGAGAILVPGTRVGAGSVIGAGSVVIQEVAAGATIVGNPARVI"
     gene            complement(3551..4627)
                     /gene="neuB"
                     /locus_tag="PLG78_10715"
     CDS             complement(3551..4627)
                     /gene="neuB"
                     /locus_tag="PLG78_10715"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016754108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HNE23634.1"
                     /translation="MSKCFIIAEAGVNHNGSLEVARKMIEVAASAGADAVKFQTFRTE
                     ALVSASAPRAEYQIRAMKGDESQAEMLKKLELDESAHSDLIAHAKQVGIEFLSTPFDS
                     LSLRLLTERFGIRTIKIPSGEITNGPFLLQIARSAEKIILSTGMSDLSEVEAALAIIA
                     FGFLNPTGNPDRSSLIEIGDSQKAWDELRNRVILLHATTEYPAPMSEVNLRAMDTLAS
                     SFGLKTGYSDHTRGIHVAVAAVARGACVIEKHFTMDRGMDGPDHKASLEPAELREMVH
                     AIRDVEQALGDGRKRPTISERKNMSVARKSLVALREIQSGQEFTEENLGVKRPGNGIS
                     PMAYWKWIGKKATRTYSAEELLNE"
     gene            complement(4624..>5511)
                     /gene="neuC"
                     /locus_tag="PLG78_10720"
     CDS             complement(4624..>5511)
                     /gene="neuC"
                     /locus_tag="PLG78_10720"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNE23635.1"
                     /translation="PDCIVLLGDRYELLSAASAALVARIPIVHIHGGETTEGAFDEAI
                     RHSITKMSHLHFVAADAYRRRVIQLGEDPQNVHVVGGLGLDGISKIALLGRSELEQAL
                     DFKLGARNLLITFHPVTLESESSETQMRALLSALDSFPDIHCIFTMPNADNDSRVIFR
                     LIEEFTARRANSKAFTSLGQLRYLSCLRHVDGVVGNSSSGLIEVPSFRKATINVGDRQ
                     KGRLRAQSVLDCNADSTAIRSCIEKIYSDEFQNLLSQTVNPYGTAGASEKIVKILEAR
                     RENRSVKKTFYDLKPGDLA"
BASE COUNT         1396 a         1540 c         1323 g         1252 t
ORIGIN      
        1 atttttcact ccagttttgt cagtatagca ttgcgtatga attcgtgcgc gggcacatcc
       61 ggatatggca taaaataggc gcgcgcgggc cctgactgtt tctcatagtc catgtcagac
      121 atgttcagca aatcgtctaa cgccctggca aaagaagaat aatccgcccg gttaatatgc
      181 cagggtccag tcgtcggagg atcgtattgg gcaagacccg agaaattaga gaacaaaact
      241 ctttgccccc agccgaatgc ctcacgaccc gccgtggatg cgctcgtcac tgcaaccaga
      301 ctctgatcca tgatccgata tgtgctccgc gaaaccggat cgaagtctgc cagatgagtt
      361 gcttcgccca gaagacgttt gtaatattcc ttctctcctg gattcggaga gcgtgtagca
      421 acacagacac gcgctttcga atttctagtc cagcgccgca gaaagtaatg aagaaccaga
      481 atcgcgcgtt tcagttccgg tatctcgaaa tcccggtgaa ctccgggata ccaatcagaa
      541 ataaagcaca catcaaactc gcgcgagctt cctgaccacg gaacgcgttt gcgatagtac
      601 cccgcgatga gagaaccaac cggatgaaac tgtttcaccg gaacttcata acggcgaaaa
      661 atatcgatat caaactgact gtagcaaaag aactccggca gaggcagaat aggttcgccg
      721 gtattccagc gttcttccac tggaagacgc gtgccattct gaactgcaca aaacgtggcc
      781 gccccggaaa actctgcaat ccaacgaaaa tggtgattgt tgtcaatatg cgtgacgact
      841 acacgggctc ccgatgcgcg aatgcaagca gtcaggtaca cgtgaaatga acgtgatgca
      901 atgtcacgat agaacctggt tcgaagaaag aagatcctta cttccagaag catgtaccag
      961 agcacgcgcg cgcccaataa atactcttcg ccgcgcaccg gcagcacaca tgcttttacg
     1021 tcaggcagac aatatcccag aagatactga cttccttcgc gatcaaaaat ggcaagcttg
     1081 ctctggattg gactgcgaaa cctcgcgttg cgcaaaacgc gcctcaggcg accggccaga
     1141 tagaaagcaa gtgccacata tctcaatagt cgcctcatgc cggatttggg cgctgcgaaa
     1201 gctggaactc ggccatagcc cagtcaagcg gagtgtcaat gtcaatggat cgatctgcag
     1261 gcatcacgta tccaaacgcg cccgattcag agaagccttt ccctgcgcgc agatgagcag
     1321 agctcgcagc gtacagagca ccattgatca catacgccgg ttcgagatcc tgacgacgag
     1381 ccgggacctt ccctccaaga attgctttca tttttccatc tctgtccagg cggtacatcc
     1441 attcaggagg agaaggtgca ggtgaaagcc caacagcgct ttgacaacca gaggcaataa
     1501 aagcctgcat aaatccatca atgtcgcctg acgtgcgaag tggcgatgtt ggctggagaa
     1561 gcaaaagaat ctcgatttct ggaaaacaat cgatggcatg ccttaccgga tccatgcccg
     1621 aagcttcatc cgttgccagc tcgaccgggc gtttcagagc ttgagcgcca agagatttag
     1681 ccaccgtgag tatctcttca tcttccgacg aaacaacaat catgtccagg aatgccgcct
     1741 gacgggccgc ttcgatggtc catccaatca ggggccgtcc gcccagaatc ttgatgtttt
     1801 ttctggggat gcccctggat cctccgcgcg ccggaatcag ggccagaacc ttcttgccgc
     1861 cgatcacgaa aatgtctcgc caaactctcc gttcgcacgg cggaaatctt cgagccctcc
     1921 aatatccagc cagtattcac gcagtggata tgcggatgtg gattggcctg ccgtcctgag
     1981 tttgtcaaac agtgttggca tatcaaagaa ttgacccgag ggaatgagtt ttagtgcagc
     2041 gggggaaatc gtgtagattc cagcatttac aaaaaacttg tgaacgggct tttcctcaat
     2101 gccccgaatt tcgtgacctt caattcggac cactccatag ggcacctgaa aatcgtattc
     2161 cttcacggcc attgtcgccg cggctttgtt ctcttcgtga aactttagca tggcgctaaa
     2221 attcgcacgc gttagaagat caccgttcat gaccagaata ggctcctccg gcacttcggg
     2281 aagaagggag agcgctcctg ccgtaccaag gctttgcttt tcgtgcagat attcaatttt
     2341 gacgccccat ttcgtgccat ccccaaaata atcagaaatg gtgtgcgcga gatagtttac
     2401 agatacatag aatctgcgga agccctgttc tgcgaaattt tctaaaatgt tttcaaggat
     2461 aggctttcca ccgacggcca ggagaggttt gggtgtatct tctgtcagag ggcgaagtcg
     2521 cttcccctga ccgcccgcca taaggacaac tcgattgggt ttctcaactg cgccaatcaa
     2581 atcatccagt gtagccagcc caacaatgcg acggtccgca tccactacag gcaaatgatg
     2641 gagcacaccc cttcgcatga tcgaaagcat ttcctcgtgc gaagtatctg gacgaacggt
     2701 cacgggattt gaattcatca cgtctcgcgc tgcaatatca agactctgac ctttcaaaat
     2761 cgcacgtcga atatctccgt ccgtaagaac accttccagc ttcccggcag gccctgccac
     2821 cagtacgagc tggatccccg tagcatcaat ctgcttgagc gcatcgagaa tcgatcgctc
     2881 cggagccaga atactttgtt cccaacttct caaatcttac ctcaaatcac acgcgctgga
     2941 tttccaacaa tcgtggcacc ggccgcaact tcctggatga caacagagcc agctccaatc
     3001 acagatccag cgccgacgcg agttcccggt accaatatgg cacccgcacc gacgaagaca
     3061 gattcctcaa gcgtcacgcc gccacagaga attgcgccag gcgaaacgta gcaatgcgcg
     3121 cccactctgc aatcgtgatc tacacgcgcc gccgtattga tcacaacgtt ttcggcgatt
     3181 cgaacgcggt tctgaattac agctccagca aggatctgcg cgctgggatc aatttcacat
     3241 tcattcccca caatcgtgtc cgggtgaagc acaccctgca ccgtgaaacc catttgaagc
     3301 cacgcggaat actgcgtgcg ccgtcgctct acattcttta ctgcgccaaa cccgttgagt
     3361 acggaattcc cgcttgctgg aagcgactcc agataagagt cattgcccag cacaggaata
     3421 ccaaaaatga gttcgcccgg agacaaggcg ccatcaatta cgccatccac ggaaatcccg
     3481 gcgcgcaagg ccgagtccag aacgactctc ccatgcccac ctccggcgat tagaagcaga
     3541 agtcctgatt tcattcgttc agcaactcct cagcgctata ggttctggtt gctttctttc
     3601 cgatccattt ccagtacgcc atgggcgaaa taccatttcc tggtctcttt acaccgagat
     3661 tttcttccgt aaactcctgg ccgctttgaa tttcgcgcag ggcaaccaga ctcttcctgg
     3721 ccacgctcat attctttctc tcagaaatgg ttgggcgttt gcgaccatcg cctaacgctt
     3781 gttctacgtc acgaatcgca tgaaccattt ccctgagttc cgcgggttcg agagacgcct
     3841 tgtgatcggg gccatccata ccgcgatcca ttgtaaaatg cttctctata acgcacgcgc
     3901 ctcgtgcaac ggcagccacc gcgacgtgaa tgccacgggt atggtcagag tagcctgttt
     3961 tcaaaccgaa ggaagaagcg agtgtatcca tcgcacgaag attcacttcg ctcatggggg
     4021 cgggatactc tgtggttgcg tgcagaagga tgaccctgtt tcgcagttcg tcccacgctt
     4081 tttgagaatc cccgatctcg atcaaagaag acctgtcagg atttcctgtc ggattaagga
     4141 aaccaaatgc tatgatcgcc agagccgctt ccacctctga aagatcgctc atgccggtcg
     4201 ataagatgat tttctcagcg gaacgagcaa tttgaagaag aaaggggccg ttcgtgatct
     4261 ctccagaagg aatcttgatg gttcgaattc cgaagcgttc cgtgaggaga cgaagactca
     4321 gagaatcaaa aggtgtagac agaaattcga tcccaacttg ctttgcgtgc gcaatcagat
     4381 cgctgtgagc ggattcatcg agctccagtt tcttaagcat ctccgcctga ctttcgtctc
     4441 ctttcattgc gcgaatctga tactcagcac gcggcgcaga tgcgctgacc agcgcttctg
     4501 tgcgaaaagt ctgaaacttt actgcatccg cacccgcact ggctgcgact tcgatcatct
     4561 ttcgcgcgac ttcgagagat ccgttgtgat tgacgccagc ttcagcgata atgaagcact
     4621 tgctcatgcg agatctccgg gcttgagatc gtagaatgtc ttcttaactg atcgattttc
     4681 gcgtctggct tccagaatct tcacgatctt ctcactcgca cctgcggtgc catacgggtt
     4741 tacggtttgc gaaagcaaat tctgaaactc atccgaatag atcttttcaa tgcaagaacg
     4801 aattgcagtg gagtctgcat tgcaatccag gacactttgc gctctaagac gacctttttg
     4861 acgatcgccc acattgatcg tagctttgcg aaaggaagga acttcaatca aaccgctgga
     4921 ggaattcccc acaacaccgt ccacatgccg taaacacgaa aggtacctca gctgcccgag
     4981 ggatgtgaac gcctttgagt ttgcgcgacg cgccgtgaat tcttcaatca accgaaagat
     5041 aacgcggctg tcattgtctg cattgggcat cgtgaaaatg caatgtatgt ccggaaaact
     5101 gtccagagcg ctgagcaacg ctcgcatctg agtctcagag gactcagact ccagcgtaac
     5161 cggatgaaac gtgatcagaa gatttcgcgc accgagttta aaatcaagcg cctgctccaa
     5221 ttccgaccgc ccgagcaaag caatcttaga aattccgtcg agacccagtc ccccgactac
     5281 atgcacgttt tgtggatctt ctccaagctg aatcacgcgc ctgcggtagg catccgctgc
     5341 aacaaagtga agatgtgaca tcttcgtaat agagtggcga atggcttcat caaacgcacc
     5401 ctcggttgtt tctccgccgt gaatatgaac gattggaatt cgtgccacaa gggcagctga
     5461 agcggccgac agaagttcat agcgatcacc gagcagtaca atgcagtcag g
//
LOCUS       DAOQJL010000226         5493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8567_length_5493_cov_4.042847, whole genome shotgun sequence.
ACCESSION   DAOQJL010000226 DAOQJL010000000
VERSION     DAOQJL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8567_length_5493_cov_4.042847"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLG78_10725"
     CDS             <1..360
                     /locus_tag="PLG78_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE23636.1"
                     /translation="KGEILDAVNMDLETCRMYPREERAGRVCLRAGKLLRRSGDVDRA
                     RSVFKEVADSFPASGVARMASVELSLMSESQNTPDQPEPAVNEQRNRDEMPTNEQKEP
                     IREEELPGQGVFDAKEP"
     gene            408..1613
                     /locus_tag="PLG78_10730"
     CDS             408..1613
                     /locus_tag="PLG78_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE23637.1"
                     /translation="MSFQMVNFKAGSYVVIEGKREALSFFIIRQGKLKTQKENPVIGE
                     DSSQILGPGDFFGVESAMSGHSRIETVVALSDCSLIQVNNDQFGLLIQKNAPVAMKII
                     RSFSVKLRSFDNTITRLSFRNAVEEDPSHLFVLGEHWMREGKTNHAAYAFQKYLQWNP
                     QGDKLSQAKIHLQSLNKPLKAPVIPETSINRTYKPDDMIFCENEPGRELYIMQNGRVK
                     ITKMVDSQEVMLAVLQTGDIFGEMALLDNKPRSASAIASENTTMLAINKANFETMVQQ
                     QPQLATRLITLLSERIWMAYRQLANLMITDPVGRLYDTLLTLAEKHHVKIAAKAAYTY
                     EIGGKDLLKMVGLDPQKDERYLMELLKSRWLKLDEGKIICMDLLELEKQVQFYKKKSS
                     MERKRDKPA"
     gene            1613..2110
                     /locus_tag="PLG78_10735"
     CDS             1613..2110
                     /locus_tag="PLG78_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HNE23638.1"
                     /translation="MWAPFRVETGAIIAPRPLPGIDGIGDRLRTAAFAEMQAERAFAR
                     AAEIYSDAPESLRSAWLHLASEENRHMNWLLGRMKELNIDIVERPVSDSLWNSLMSCK
                     DARSFCHYIADSEQRGRRAGLRFYDVLKDIDPTTAEIFSKIAEEEIRHIELAFQFYPI
                     ETASR"
     gene            2169..3035
                     /locus_tag="PLG78_10740"
     CDS             2169..3035
                     /locus_tag="PLG78_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HNE23639.1"
                     /translation="MPRSFFLFVRVLVPLSLAFLLTCSPTGGFTAGDLAQDYGIAAVA
                     ACGLRTARAQAGRTFTTGFEQVSDFNGFYITPSPYQGAVTHGLSTAQVHGGSNSHLAT
                     ISNAATSCTGNCNHRGYPTIQLHKTAAGGMSGVVFTEFYVYVTMTINDGQWLSLATLT
                     PDSSDAWSRTVLVNVGNLGRTGGGNYMHLMHVPNQGESGWTYQAGSTNGGTQFPQNQW
                     VKVSICLDLNPATGWAKAWMNDVPVSAAPVSGGCGVLEQAHFGLYAPPAISSGVIYND
                     DLQIREVSVCPK"
     gene            complement(3037..3441)
                     /locus_tag="PLG78_10745"
     CDS             complement(3037..3441)
                     /locus_tag="PLG78_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNE23640.1"
                     /translation="MTESGMQRARVIKWPHKETPTKEAVYKELEMHGFQVYDLQTIPP
                     WCERSLHTHDYPEIRGAVAGVTTFHFPEGPVTIEAGDILFIPAGVPHSLRTHNGNEFT
                     AYKGSINGIRKVTELGDGKNSVEYLAKLKDAQ"
     gene            3547..>5493
                     /gene="scpA"
                     /locus_tag="PLG78_10750"
     CDS             3547..>5493
                     /gene="scpA"
                     /locus_tag="PLG78_10750"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNE23641.1"
                     /translation="MANFADWQKQLAKDLKGTTDQDLTTMTPEGIPLKPLYTAADLAA
                     TDQEAVGSLPGLYPFLRGPYTSMYSHRPWTIRQYAGFSTAEESNAFYKRNLAMGQKGL
                     SVAFDLATHRGYDSDHPRVKGDVGKAGVAIDSVEDMKILFSDIPLADISVSMTMNGAV
                     IPVLACFIVAGEEQGVGPDKLAGTIQNDILKEFMVRNTYIYPPDPSMRIVSDIIEYTA
                     HRMPKFNSISISGYHMQEAGATCELELAFTIADGLEYVKAALARGLDIDEFAPRLSFF
                     FAIGMNFYMEIAKLRAARVLWARHMKVFNPKSEKSLLLRTHCQTSGVSLTEQDPYNNV
                     IRTAIEAMAAVFGGTQSLHTNALDEAIALPTDFSARIARNTQLIIAEETGIPRVIDPW
                     GGSYFMEKLTQDLVDRAEALIKEVNELGGMTKAILSGMPKRKIEECATRHQAQVDKGD
                     KTIVGVNKYRAKTQDPMEILEIDNADVLRKQIARLEKIRSSRDAGAVKAALGAIEEAA
                     KSGKGNLLELAVNAARLRATVGEISDAMETSFGRYAAPIQAISGVYAAHFAENEEWKL
                     IRTEVEKFAKDKGRRPRILIAKMGQDGHDRGAHVVATSLSDLGFDVDIGALFMTPQEV
                     AKSAVENDVHVVGVSSLAAGHKTLV"
BASE COUNT         1398 a         1369 c         1423 g         1303 t
ORIGIN      
        1 aaaggagaga tcctggacgc agtgaacatg gacctggaga cctgtcgcat gtatccgcgc
       61 gaagagcgcg cgggcagagt ctgtttgcga gcaggaaagc tcctgcgccg gtcaggcgat
      121 gtagaccgcg ccaggtctgt ttttaaggaa gtcgcggaca gtttccctgc ctctggagtt
      181 gccagaatgg cgtccgtgga gctcagtttg atgagcgaat cgcagaacac gccagatcag
      241 cctgagcctg ccgttaatga gcaaagaaat agagacgaaa tgccgacaaa tgaacagaag
      301 gagccaattc gggaagagga attacctgga cagggcgttt ttgacgcaaa ggaaccttga
      361 ttttcttgtt gtcactagtg ccaccggaaa cacccctgag ataagccatg tcctttcaaa
      421 tggtcaactt taaagcgggc tcctatgtgg tcatagaggg caaacgcgaa gctctgagtt
      481 tcttcatcat ccgtcagggc aagctgaaga cccagaaaga gaaccccgtc attggcgagg
      541 acagttccca gatccttggt cctggggatt tctttggcgt ggagagcgcc atgtccggcc
      601 actccagaat tgaaactgta gtcgcactgt ctgactgctc tctcatccag gtaaacaacg
      661 atcaattcgg acttctcatt cagaagaacg cgcctgttgc gatgaagatc attcgatctt
      721 tctctgtaaa gctcagatca tttgataaca cgattacgcg tctttcattt cgcaacgcag
      781 ttgaggaaga tccttcgcat ttatttgttc tgggcgaaca ctggatgcgc gaaggcaaaa
      841 caaatcacgc agcttacgcg tttcagaagt atctacagtg gaatccgcag ggcgacaaac
      901 tcagccaggc caaaattcat cttcaatctc tcaataagcc tctgaaagct ccagtcattc
      961 cggagacatc gatcaatcgt acgtacaaac cggatgatat gatcttttgc gaaaacgaac
     1021 ccgggcgtga actgtatatc atgcagaacg gcagagtgaa gatcacaaag atggtggata
     1081 gtcaggaagt gatgctcgca gttttgcaaa ccggtgacat attcggtgag atggcgcttc
     1141 tggacaacaa gccccgcagt gcgtctgcca tcgcatctga aaatactacc atgcttgcca
     1201 tcaacaaagc aaactttgaa accatggtgc agcagcagcc acagcttgcc acgcgtttga
     1261 tcactctgct ttcagaaaga atctggatgg catacagaca acttgccaac ctgatgatca
     1321 cagatccggt gggtcgtctg tatgacactc tgctcactct tgcagagaag catcacgtta
     1381 agattgcggc caaggcagca tacacttacg aaatcggcgg aaaggatcta ctgaagatgg
     1441 ttggtctgga cccgcaaaaa gacgaacgct atctcatgga actcttgaag tctcgctggc
     1501 tcaaactcga tgaaggaaaa atcatctgca tggatcttct agaactggag aagcaggtgc
     1561 agttctacaa aaagaaatcg agcatggagc gcaagagaga caagccagcc tgatgtgggc
     1621 cccctttcgc gtcgaaacag gcgcgatcat cgcaccaaga ccacttcctg gaatcgacgg
     1681 aatcggagat cgtcttcgca cggctgcttt tgcagagatg caggcggagc gtgcttttgc
     1741 tcgcgccgct gaaatttatt cagatgcgcc cgaatcactg cgaagtgcat ggttgcacct
     1801 tgcttccgaa gagaatcgcc atatgaactg gctgctgggt cgcatgaaag aattgaacat
     1861 tgacatagta gaaagacccg taagcgattc cctctggaat tcgctcatgt cgtgtaaaga
     1921 cgcgcggagc ttctgtcatt acatcgcaga ttctgagcaa agaggaaggc gcgcaggcct
     1981 tcgtttttat gacgtattaa aagacattga tcctacaacg gctgaaattt tttcgaagat
     2041 cgcggaagaa gaaattcgtc acatcgaact tgcgtttcag ttctatccga ttgagaccgc
     2101 gtcccgttaa gcgcaattgc ggacaaaacg gtttgactca tttcgccaga tcgtcgcaaa
     2161 cttcaaccat gccacgttcg ttcttccttt tcgttagagt gctcgtccct ttgagccttg
     2221 cgttcctcct cacttgcagt ccaacaggcg ggtttacggc gggggattta gcgcaggatt
     2281 acggaattgc ggcggtggct gcctgcggac tccgaaccgc tcgtgctcag gcaggcagaa
     2341 cgtttacaac tggattcgaa caggtaagcg atttcaacgg cttctacatc acgccttcgc
     2401 cttaccaggg tgctgtgact cacggacttt ccacagccca ggtgcatggc ggaagcaatt
     2461 ctcatctagc aactatttcc aacgctgcaa caagctgcac tggaaactgc aatcatagag
     2521 gatacccaac cattcagctt cataagaccg ccgcaggcgg aatgtcaggt gttgttttta
     2581 ctgagttcta tgtgtatgtt actatgacca tcaacgacgg acagtggctg agcctggcaa
     2641 ctcttactcc tgattcttca gatgcctgga gtcgcaccgt gctcgtgaat gttggaaacc
     2701 tgggacgcac agggggcggc aattacatgc acttgatgca cgtaccaaac cagggggaga
     2761 gcggctggac gtatcaggca ggctctacga acggtggaac gcagtttccg cagaaccagt
     2821 gggtcaaggt ttccatctgt ctggacttaa atccggcgac gggctgggcc aaagcatgga
     2881 tgaatgatgt gcctgtgtct gcagcaccag tctcgggagg ttgcggagta ctcgaacagg
     2941 ctcattttgg attgtatgct ccgcccgcga tttcaagtgg agtcatatac aacgatgatc
     3001 ttcagatcag agaagtctct gtctgtccaa agtgaatcac tgcgcgtctt ttagttttgc
     3061 gagatactct acgctgtttt ttccgtcacc cagttccgtg actttgcgaa taccgttgat
     3121 cgagcctttg tatgctgtga attcatttcc gttgtgcgtt ctaagggaat gcggaactcc
     3181 agctggaata aaaagtatgt ctcctgcttc aatggtaacc ggaccttccg gaaaatgaaa
     3241 cgtggttaca ccagccactg ctccgcgaat ttcagggtag tcatgagtgt gcaggctacg
     3301 ctcgcaccag ggcggaatcg tctgcagatc gtatacctgg aatccgtgca tttctagttc
     3361 tttgtatacc gcttcttttg ttggagtttc tttgtgcggc catttgatga cccgggctct
     3421 ttgcattcct gattctgtca catttgcgcc tccaggtctt tgacgcccgt caggtctcga
     3481 tgatgacttt cgtcacacgt gctgttttcg gcttttcttg cgcacgcgcg ggagaatccc
     3541 tgcatcatgg cgaatttcgc agactggcaa aagcaacttg caaaagatct aaaaggcaca
     3601 accgaccagg acctgaccac catgactccg gaaggcattc cgctgaagcc cctttacact
     3661 gcggcggatc tggcggccac agatcaggaa gctgtaggat ctctacccgg gctttatccc
     3721 tttcttcgcg gcccatacac ttcgatgtac agccatcgcc catggacgat tcgccagtat
     3781 gcaggcttct ccactgcaga agaatcgaac gcattctata aaagaaacct ggcgatgggg
     3841 cagaagggtc tgtccgtggc gtttgacctg gccactcatc gtggttacga ctcagatcat
     3901 cccagagtga aaggagatgt tggaaaggca ggcgttgcga tcgattcagt cgaggatatg
     3961 aagatcctct tcagcgacat tcctctggca gacatttcag tttctatgac catgaatggt
     4021 gcggtgattc cagttctcgc ctgtttcatt gtcgcaggag aagagcaggg agtgggtccg
     4081 gacaaactcg ccggcacaat tcaaaacgat atcctcaaag aattcatggt gcgaaacacc
     4141 tacatctatc cacccgatcc ttccatgcga atcgtgtctg atatcattga atacacagcg
     4201 catcgcatgc caaagtttaa ttcgattagc atttcaggtt accacatgca ggaagcagga
     4261 gcgacctgtg aactggaact cgccttcacg atcgcagatg gtcttgagta tgtaaaagca
     4321 gcgctcgcgc gcggattgga tatagacgag tttgcaccca ggctgagttt cttttttgca
     4381 atcggaatga acttttacat ggagattgcg aaactgcgtg cggcacgcgt tctctgggcg
     4441 cgccacatga aagtattcaa cccgaaatca gaaaaatctc ttctccttcg cacgcattgt
     4501 cagacaagcg gggtaagcct cacagaacag gatccgtaca acaatgtgat ccgcaccgca
     4561 atcgaagcca tggccgcagt gtttggcggc acacagagtc ttcacaccaa cgctctagac
     4621 gaagcaatcg ctcttcccac tgattttagc gctcgaattg cgcgaaacac gcagctcatc
     4681 attgcagagg aaactggaat tcctcgcgtg attgatccat ggggcggcag ttacttcatg
     4741 gaaaaactca cgcaggacct ggtcgatcgc gccgaggctt tgatcaaaga agtaaacgaa
     4801 ctcgggggaa tgacaaaggc gattctttcc ggaatgccaa agagaaagat tgaagaatgt
     4861 gccactcggc atcaggccca ggtggataaa ggcgataaaa ctatcgtagg tgtgaacaag
     4921 tatcgcgcga agacgcagga tccaatggag attcttgaaa tagacaatgc agatgttctg
     4981 cgcaagcaga ttgctcgtct ggaaaagatt cgttcttcgc gcgatgcagg agctgtcaaa
     5041 gcagcactcg gtgccatcga agaagcagct aaatccggca aaggcaatct tctcgaactc
     5101 gctgtcaacg ccgccaggct tcgtgctacc gtgggagaaa tttcagacgc catggaaaca
     5161 tcttttggac gttatgcggc acccattcag gctatttctg gagtgtacgc ggcgcacttt
     5221 gctgaaaatg aagagtggaa actcattcgc accgaagtgg agaaatttgc aaaagacaaa
     5281 gggcgcagac cccgtattct cattgcgaag atgggccagg acggtcacga tcgtggagct
     5341 catgtagtgg ccacttctct gtctgacctt ggatttgatg tggacattgg agccttgttc
     5401 atgactccgc aggaagtggc caagtcagct gtggaaaatg acgttcatgt ggtaggagtg
     5461 tcgtctctgg ccgccggtca caaaacgctc gtt
//
LOCUS       DAOQJL010000227         5476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8602_length_5476_cov_4.258624, whole genome shotgun sequence.
ACCESSION   DAOQJL010000227 DAOQJL010000000
VERSION     DAOQJL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8602_length_5476_cov_4.258624"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLG78_10755"
     CDS             complement(<1..1368)
                     /locus_tag="PLG78_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE23642.1"
                     /translation="MAEDRYLELFLEEAGEQIEELSQALLRLEKEQTNLEVINDVFRN
                     AHTLKSSAAFVGLDDLSLLAHHMEDVLHMIRSGALQVTTPMVSLLFRCLDRIKSAVAV
                     VHGGGRPDDRFADLIQALDEVKPAGGAKAPATSPSQASASVTSSASPSQSADIPSSVL
                     SGAVAAPYSTGVPSAPYSAESSDSFELSFGEQEMLKSQAGTSRIYEGLVRLDPESPMK
                     GLRFMLLSQNLKKAGSIFKCSPSEAELDSGSSSNTFRFFFYGDISSAELTKLCQVDMV
                     EEVRVSEKTFGKLDAGKPAQEETAVKTRNIKVSSEKIDYLLNSVGELVITNSGLQKTY
                     EDLQGEYRDSPLLAELKSRIDQAARIARDLQSGIMKTRMIPVGLVFHRFTRPVRDLAL
                     ELGKEVEFEFHGEETELDKNIIDGLNEPLLHIVRNSLDHGIETPEQRRAAGKPPVATL
                     GMHAYQ"
     gene            complement(1361..1855)
                     /locus_tag="PLG78_10760"
     CDS             complement(1361..1855)
                     /locus_tag="PLG78_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE23643.1"
                     /translation="MNKEIFSGHIEGLDLDSEKETELVQFLVFQCGDGIYGINILDTH
                     EILKPVPVTRLPNVDPEYLGVLNLRGNIIPLLDANRKFGDTYSEITNLTRIVVCASGG
                     KFLGILVDRILEVARLPSDRIEGREVSGLSHQYVSAVGRSDARLFLILNLQILSGKAE
                     EENG"
     gene            complement(1848..2357)
                     /locus_tag="PLG78_10765"
     CDS             complement(1848..2357)
                     /locus_tag="PLG78_10765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNE23644.1"
                     /translation="MAQKEFHSFLQDGRAASAILHLRTFVQAQPYRFILAFAALPSEL
                     DVLQAILPPYYLPRTLPGKQMAFHLTRAGDALEAGPYGILQPSAENPQFEMPAGREDL
                     VIVPSLAVNKEGCRLGRGGGYYDRARDQLVRAKIVSVLPDALAGLDFPGESHDLKVTT
                     VITESGIHE"
     gene            complement(2392..2697)
                     /locus_tag="PLG78_10770"
     CDS             complement(2392..2697)
                     /locus_tag="PLG78_10770"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNE23645.1"
                     /translation="MSPAKTTVSILGETFTIRGEASEDYIAEIARMVDSTMRDIRGQQ
                     QNLDRFKLAVLAAINIADELMRRKQEPEIKPEEEFTRRTSQLIALLDEGLVGERLAG"
     gene            complement(2694..3500)
                     /locus_tag="PLG78_10775"
     CDS             complement(2694..3500)
                     /locus_tag="PLG78_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23646.1"
                     /translation="MITLQQLDELEGRIVRALELIGDLRSENSRLESENEHLRSEHDQ
                     LKLALEQKEKEMAGLRTQLDQTGAELKELRSKEEVLEKKITEMLSRLTDMEKGRPISS
                     ASSHSIASSAPAASVAPAAVAAAPVAHVVTPAPARAEEDESVVIIEDVPPSKPAAPAP
                     AAAAAVAPAPAPMAAAPVHHAEPEEEIIILDDEEDDIVLSQEDELEIISEDLPHDKAA
                     PAAAVAPAPAPAAHAHTHADEDIVLEDDEESLGVFDVEDEDDFLIVEEDK"
     gene            complement(3595..3948)
                     /gene="rplT"
                     /locus_tag="PLG78_10780"
     CDS             complement(3595..3948)
                     /gene="rplT"
                     /locus_tag="PLG78_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNE23647.1"
                     /translation="MRVKGGPNHKNRRKSLMKQAKGFRAGAGRLYRTAIQAVIHAEEH
                     RFASRKQFKRSMRSLWIVRINAACRESGVSYSRFLHGLKKSGIDMNRKMLADLAVTDA
                     AALKALIARVTQKAA"
     gene            complement(3949..4152)
                     /gene="rpmI"
                     /locus_tag="PLG78_10785"
     CDS             complement(3949..4152)
                     /gene="rpmI"
                     /locus_tag="PLG78_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNE23648.1"
                     /translation="MSKLKTNKSAAKRLRLTSSGKVKRRRAFGNHILTKKSAKRMRHL
                     KKTTLINIADRKAIQDLLPYGGR"
     gene            complement(4248..4775)
                     /gene="infC"
                     /locus_tag="PLG78_10790"
                     /pseudo
     CDS             complement(4248..4775)
                     /gene="infC"
                     /locus_tag="PLG78_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            5004..>5476
                     /locus_tag="PLG78_10795"
     CDS             5004..>5476
                     /locus_tag="PLG78_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNE23649.1"
                     /translation="MISNILFAYVGGLLLNLMPCVLPVLSMKVLHVMEGGEKRSHSIF
                     YILGILISFWILFGVLSALKASGRALGWGFQLQSPVFVAFTAIVFLLLALNLFGAFEW
                     GAWLQQKAGTAEASTRTLGGAPQAFLTGVLAVFVASPCSAPFMGPALAYALSAPAL"
BASE COUNT         1200 a         1379 c         1429 g         1468 t
ORIGIN      
        1 ctgatacgcg tgcataccca gagtcgcgac cggaggcttc cctgcagcgc gtctttgttc
       61 aggagtttca attccatgat ccagcgaatt tcgcacaatg tgaagaagag gttcgttcag
      121 gccatcaatg atgtttttgt cgagctcggt ttcttctccg tgaaattcga attcaacttc
      181 ttttccaagt tcgagagcca ggtccctcac aggacgagtg aagcgatgaa acacaagccc
      241 gaccggaatc atcctggtct tcatgatgcc tgactgtaag tctcgggcga tgcgcgccgc
      301 ttggtcgatc ctacttttga gttctgccag aagtggcgaa tcgcgatact cgccctgcaa
      361 atcttcataa gtcttttgca gtccagaatt cgtaattacg agttctccaa cactgttgag
      421 caaatagtct attttttccg aagagacttt gatattacgc gtttttaccg ctgtctcttc
      481 ctgggctggc ttaccggcat cgagcttgcc aaatgttttc tcagatacac ggacctcttc
      541 caccatgtcc acctgacaga gtttggttaa ctctgcagaa gagatatcgc catagaaaaa
      601 gaatcgaaac gtattggaag agcttccaga atcaagctct gcttctgatg gagagcattt
      661 gaaaatcgat cctgctttct tcagattctg cgaaagaagc atgaatcgca atcccttcat
      721 aggggattca ggatcgagcc tgacaagtcc ttcgtaaatt cttgaagtgc ctgcttgcga
      781 cttcagcatc tcctgttcgc cgaaagacag ttcgaatgag tcagatgact ctgcggaata
      841 gggagcagat ggaactccgg tagaataggg agcggccact gcgcccgaaa gaacagaaga
      901 aggaatgtct gcagattgcg aaggcgatgc ggacgaagtg acggacgcag aggcctgcga
      961 aggtgaagtt gcaggtgctt tcgcacctcc cgcaggtttt acttcatcga gtgcctgaat
     1021 caaatcggcg aagcgatcat ccggacgacc gccaccatgc acgacagcca ccgcggattt
     1081 aattctgtcg aggcagcgaa acaacaggct gaccatggga gtcgtgacct gaagagcgcc
     1141 ggatcgaatc atatgaagaa catcttccat atgatgcgca agcaaagaca gatcatcgag
     1201 accaacaaag gcggcagaag atttcagagt gtgcgcgttt ctaaagacat cgttgatcac
     1261 ttcgagattg gtctgttctt tttccagacg aagaagcgcc tgactgagtt cttctatttg
     1321 ctcccctgct tcttcgagga agagctctaa ataacgatct tcagccattt tcttcttctg
     1381 ccttgccaga aagaatctgg agattcaaaa tcaaaaacaa tctcgcatcc gatctcccca
     1441 ctgcactgac gtactggtgc gaaagaccgc tgacttcccg gccctcgatt ctgtcggaag
     1501 gaaggcgagc cacttccagg attctatcta ccaggattcc gaggaatttg ccgccgctcg
     1561 cgcagaccac gattcgcgtg agattggtaa tttcagagta tgtatctcca aacttgcgat
     1621 tggcatctag aagcggaatg atattgccgc gaagattcag cacgcccagg tattccggat
     1681 ctacgttggg taagcgagtc acaggcactg gcttgaggat ctcatgggtg tccagaatat
     1741 tgatcccata gatgccatct ccgcattgaa atacgagaaa ttgaaccagc tctgtctctt
     1801 tctcagagtc cagatcgagt ccctctatat ggccgctaaa gatctcttta ttcatgtatt
     1861 cctgattccg tgatcacggt ggtcactttc agatcgtgcg attctcctgg aaagtccagt
     1921 cctgccagag catccggcaa gacagataca atcttcgctc gcacgagctg atcccgggcc
     1981 cgatcgtaat acccaccgcc gcgacccaga cgacaacctt ctttattaac ggcaagtgaa
     2041 ggcacaatta ccagatcttc ccggcccgca ggcatctcga actgaggatt ttctgcggat
     2101 ggctgtaaaa taccgtacgg cccggcttcc agcgcgtcac ctgcccgggt cagatggaat
     2161 gccatttgct taccaggcaa ggtcctgggc aaatagtacg ggggcaaaat ggcctggaga
     2221 acgtctagtt cagaaggcag ggctgcaaat gctagaatga atctgtaagg ttgtgcctgg
     2281 acgaacgtgc gaaggtgaag tatggcgcta gcggcgcggc cgtcttgtag aaaggaatga
     2341 aattcttttt gggcgatggc cctggcttct atcttggaaa gaatgctagc ctcagccggc
     2401 caggcgttcg cctacgaggc cttcatccag aagagcgatg agctggctcg tgcgacgagt
     2461 gaactcttct tctggtttga tttctggttc ttgtttgcga cgcatcagtt catctgcgat
     2521 gttgatggct gcaagcactg cgagtttgaa acgatccaga ttctgttgct ggccgcgaat
     2581 gtcgcgcatg gtagaatcca ccatacgcgc aatctcagcg atgtagtcct cggatgcttc
     2641 accacgaatg gtgaaggttt ctccgagaat gctcactgtg gttttggctg gactcactta
     2701 tcctcttcta cgatgagaaa atcgtcttcg tcttctacgt cgaagacgcc caggctttct
     2761 tcgtcgtctt cgagtacgat gtcttcatct gcatgcgtgt gtgcgtgtgc tgccggggct
     2821 ggagcaggag ctacagccgc cgctggcgct gctttgtcat gaggaagatc ttcagagatg
     2881 atttcaagtt catcttcctg cgaaaggacg atgtcatctt cctcgtcgtc gaggatgatg
     2941 atttcttctt ccggttcagc atggtgaact ggagctgcag ccatgggggc cggagcagga
     3001 gcaacggcag ctgccgccgc aggagcaggt gctgctggct tggatggcgg aacgtcttca
     3061 atgatgacaa cgctttcatc ttcttccgct ctggctggag caggagtcac tacatgcgct
     3121 acgggagccg ctgcaaccgc tgcgggtgcc acggatgcgg ccggtgccga ggaagcaata
     3181 gaatgagatg atgcgcttga aatcggtctg cccttctcca tgtctgtgag acggctgagc
     3241 atttctgtga ttttcttctc gagcacttct tctttggagc gaagctcctt gagttcagcg
     3301 cctgtctggt ccagctgcgt gcgcaggccg gccatttctt tttctttttg ctccagcgca
     3361 agtttcagct ggtcatgctc tgagcggaga tgttcgtttt ctgattcgag gcgggagttc
     3421 tccgaacgaa gatctccaat tagctccagg gcgcgaacaa ttcgcccttc cagttcatca
     3481 agttgttgca gtgtgatcat ttttgccgcc caaagatact ataacagggt tacagtattg
     3541 aacggcaaga gatcacagaa caagtagatt ttttttgcct ggtatgaaaa aaactcaggc
     3601 cgctttctgg gttactctgg cgatgagcgc tttgagggcc gctgcatctg taactgccag
     3661 gtcagcgagc attttgcgat tcatgtcaat tccggacttt ttaagaccgt gcagaaaacg
     3721 agaatagcta actccgcttt cgcggcatgc agcgttgata cgaacaatcc acagagagcg
     3781 catggagcgc ttgaactgct tacgagatgc aaaacgatgc tcttctgcgt ggatgactgc
     3841 ctggattgct gtgcgataaa gtcttccggc gcctgcgcgg aagcctttcg cctgcttcat
     3901 caggctcttt ctacgattct tgtggttggg gccgccctta acgcgcattt atctgcctcc
     3961 gtatggaaga aggtcctgga ttgccttacg gtccgcgatg ttgatgagag tggtcttttt
     4021 cagatgacgc attctcttcg cagatttctt cgtgaggatg tgattcccga aggcgcgacg
     4081 acgcttcact ttgccagaag atgtgaggcg aaggcgtttt gccgccgatt tgttggtctt
     4141 taacttggac ataattgcac ctttcaggtc tgttggccag atttctctac ctgctccgcc
     4201 ggtgcaactg gttttggctt tgtatctacc tttttcttca aaccaggctt ggctgagagc
     4261 accattacaa tctgcttgcc ttccattttg gcgggggttt cgatcgtggc cacctcagtg
     4321 acatctgtca gcaatcgatt catgatggcc atgcccagtt ccgggtgagc gatttcgcgt
     4381 ccccgaaaac gaagagtgac ttttacctta tctccctttt ctagaaagga aacggcctga
     4441 ttcttcttga tgtcgtagtc gttcttgccg acttttgggc gcagcttgat ctctttgatc
     4501 tggatgacgt gctggttttt ggcctgctct ttcttccgtt tctgttgctc gaatttccat
     4561 tttccgaaat ctacaatctt gcaaacgggt ggatcctggt cgggggaaat ttcaactaga
     4621 tccaggccgc gggcttctgc ctgttcaatg gcagtgcggg tatctactac ttctgtgccc
     4681 ttgccttcta ctacgagcct gaccttaggg ttagtaattt gacggttgat gcggggatcc
     4741 ttggacgatg gtggcctgtt tctatgctgc tgcaatgaac tctcctgaag tttggaacga
     4801 attttgggga cgaaatgaag aggaagacat catgagcgtg caagtgccag agcgcgtgaa
     4861 gaatcaggca ggtgaagaga aatgagtact tgcattcggc gaaaataggc tctaaccagc
     4921 gtgtgccgta gaaagccaaa ttccgcaagc acttttcgtt gtagaacgcg ccgcgaatcc
     4981 tttctttgac tgtagtagtt cccatgattt cgaacatcct attcgcttat gtgggcgggc
     5041 ttctcctgaa tctgatgccc tgcgttctgc cagttctgtc catgaaagta cttcatgtga
     5101 tggaaggcgg agaaaagcga agtcactcca tcttctatat acttggaatt cttatatctt
     5161 tctggattct gtttggagtg ctctctgccc tcaaggcgag cggtcgcgcc ctcggatggg
     5221 gcttccaact ccaatcgcct gtattcgttg cgttcactgc gatcgtgttt ctgcttcttg
     5281 ctctcaacct gtttggagca ttcgaatggg gcgcctggct gcaacaaaaa gcgggaaccg
     5341 cggaagcttc cactagaact ctgggaggcg cacctcaagc gttcttaacg ggtgtgcttg
     5401 ctgtattcgt cgcgtctccc tgcagcgctc cctttatggg gccggccctg gcctatgctc
     5461 tttctgcacc ggcgct
//
LOCUS       DAOQJL010000228         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8701_length_5435_cov_3.854647, whole genome shotgun sequence.
ACCESSION   DAOQJL010000228 DAOQJL010000000
VERSION     DAOQJL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8701_length_5435_cov_3.854647"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1122
                     /locus_tag="PLG78_10800"
     CDS             100..1122
                     /locus_tag="PLG78_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE23650.1"
                     /translation="MKQILQLSLAQLRGFLREPGILFWAFAFPILMAWVLGAAFDKKG
                     EGPVSVSLVQTSPAALRPTAGASLKKDPLIHLREESADAALLALRRGESQIIVEENSA
                     GVKVRFDPANQEAVLARYRAERALSKNQLPVNVEQITARGSRYVDFLVPGLVALGIMN
                     SCMWGIGWLMIEFRMKKLLRAMIAASMKRIQYLISFFVTRIIANTVETGLLLLFAGLY
                     FGIRIEGTLLAFMMVIVAGLVAFTGIAILGACRAASTQVGNGILNAVTLPLMILSGVF
                     FSYHNFPDAVVPIIRYLPLTLLADSLRAVFVEGAGVAQVWLSCAILCITGAVTFVAGL
                     RFYRWY"
     gene            complement(1123..1479)
                     /locus_tag="PLG78_10805"
     CDS             complement(1123..1479)
                     /locus_tag="PLG78_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23651.1"
                     /translation="MSSNPYSPPDTKAPLEEGERLLTPTGILRFWEIGRIPYNGILFA
                     EAVFVSQGIPKMAEIAFYGIIINALYCFGPFVDFYFLAIAHHPLPRILRYALYVLGTI
                     LCAGSMFVILAAASAS"
     gene            complement(1479..2306)
                     /locus_tag="PLG78_10810"
     CDS             complement(1479..2306)
                     /locus_tag="PLG78_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE23652.1"
                     /translation="MSLREYTIRNQRFLGISSGGVETSMILPTFHLAFDAGRCQNQLV
                     EIPTVLLTHGHLDHASGIPYYVSQRCLRHLPGGTIYCPPELEDPLRRILRIWSEIEDY
                     QMEFSLQGVDYSTFYPLFGNFSFRAVRSVHRVASNGYTIYERVRKLRKEYSHLTGPQI
                     AEMKKTMNLFEDVQSPVVTFSGDTQIEFALENEDVRRSKILFLECTYIDEARPVERAR
                     KWGHTHLFEIIENAEAFKEVEHLVLIHFSPRYSKDRILDTLRKQMPEDLYRKTTAFV"
     gene            complement(2314..3759)
                     /locus_tag="PLG78_10815"
     CDS             complement(2314..3759)
                     /locus_tag="PLG78_10815"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE23653.1"
                     /translation="MKRSLILFLLPAALMAQQTRPPANLSSKESREHAEKLIADIPST
                     PIRFAPPPVRQTTLPSGATMLVTEEKSLPFLTVELHFKGGNSLEKVDHAGLWDAATSL
                     LAVDGAGSRSGDEIAEEMSRLGARFSIRGDHESWVVSITALKWNFPKAFAIVSDILLK
                     PKLSEERLAVILDGMETDIDQRNNRPESIAGRRLLEVMYPNSRRGYVLQKTDLKKITP
                     QLIREDLNRRLYAPGVIVACAGDVSSVPVESMVNGLLAQFPAKSAQEQIQKPAGAPLL
                     AGKILLVTAEVPQASVAMGTILPGHSSEDFYALQVANHILGGNSFNSRLMKEVRTARG
                     LAYYSMSRTTFFKDDGRLTAASGTRSEQANETVKLMLEIIGGMRQPADAVELNLARES
                     ILNSLVFLYDDPESFLQSQVRFKLHGMPDDYLARYADHIRALTAEDVSRAYARHVHSD
                     MTIVVVGPKSLAPELQKIRPVVMVTPEGKLP"
     gene            complement(3756..5294)
                     /locus_tag="PLG78_10820"
     CDS             complement(3756..5294)
                     /locus_tag="PLG78_10820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNE23654.1"
                     /translation="MTSRIVFVLLSIFALPLAAQSELDSTMKSLRTDVRRVTLENGLR
                     IIMVRRPFSPTVACYMKFKAGGADETEESAGIAHMLEHMLFKGTERVGTLDFEKEKKY
                     IEQSNVWADRLDDARRALDAAEKSGDTTRIAEAKAEVQKRKQRLMSLQQMARKYMIPD
                     EDSYIYAQAGERGYNAYTTKDLTNYQVELPANRLEIWAKLESDRMENSVLRDFYTERE
                     VVREERRLRVDNVAQGALLEQFIKDIYGSHPYGRSLIGSMQVIQYLNFGQAKSFYNTY
                     YAPNNTVIALVGDIDLAQTEALMRKYFSHLKRKDIPERPSVAVAPSRARTEIEKPGSP
                     VMFLAWNKPSGMTKDSLSLEVLSSILAGRSDSRLVRKLVMKDRSAAEVSAFTGYPGER
                     HPNLLTIQAVPAPGKSYDDVQKGVLEEIASVQKDGVTQDELDRVKASMVADFLYGLRS
                     NAEYADNLSYYELLYGNYETLFTKITALSAIAPADVKKAAIEYMTPDKMHSARLKQAK
                     GEEQ"
BASE COUNT         1210 a         1311 c         1423 g         1491 t
ORIGIN      
        1 tttatccgcg gcaaagtctg cgggaatcac tgacatcgaa tgcagaagaa gtaccctgga
       61 cgatgttttc atccatatga caggaaggac tttgaacgaa tgaaacagat tttgcagctc
      121 tctctcgctc agttgcgcgg tttcctgaga gagccgggca tcttattctg ggctttcgca
      181 tttccgattc tgatggcctg ggtgcttggt gcagcattcg acaaaaaagg ggaaggccct
      241 gtgtctgtgt ctctcgtgca aacgagtcca gcagcccttc gtccgactgc cggtgcctct
      301 ctgaaaaagg atccattgat tcaccttcgc gaagaaagtg cggatgcagc tcttcttgct
      361 cttcgccgcg gtgaatctca gatcatcgtg gaagagaatt cagctggagt gaaagtgcgg
      421 tttgatcctg caaaccagga agcggttctc gcgagataca gggcagaacg cgctctatcc
      481 aaaaaccagt tgcctgtgaa tgtagaacag atcaccgcaa gagggagccg ctatgtggat
      541 ttcctggtcc ctggtctggt cgctcttgga atcatgaatt catgcatgtg gggcattggc
      601 tggctgatga ttgagtttcg catgaagaag cttcttcgcg cgatgattgc tgcttccatg
      661 aaacggattc agtatttgat ttcctttttt gttaccagaa tcatagcgaa cactgtggaa
      721 acaggtctct tgctgctgtt tgctggattg tattttggaa tcagaataga gggaacgctg
      781 cttgctttta tgatggtgat tgtggccgga ctagtcgcct ttaccggcat cgcgattctc
      841 ggagcctgtc gtgccgcaag cacccaggtg ggaaatggaa ttctaaacgc agtgactctt
      901 cctctgatga ttctttccgg tgtgttcttc agctatcata attttccgga cgcagtggtt
      961 ccgatcatac gttatttgcc attgactctt cttgcagatt cactccgtgc cgtgtttgtg
     1021 gaaggagcag gagtggctca ggtatggttg tcctgcgcga ttctttgcat taccggtgcg
     1081 gtcacatttg ttgcggggct gcgattctat cgctggtatt gatcaactcg cggaagccgc
     1141 cgccagtatt acaaacatgg aaccggcgca caggatggtt cccaaaacat agagtgcata
     1201 tcgcaggatt cgcgggagtg gatgatgcgc aatcgccaga aagtaaaagt ccacaaaagg
     1261 gccaaagcaa tacagtgcgt ttatgatgat cccgtaaaac gcaatctctg ccatctttgg
     1321 aattccctgg gagacaaaga ctgcctctgc gaatagaatg ccgttgtacg gaattcttcc
     1381 aatttcccag aagcgcaaga tgcccgtggg cgtcaaaaga cgctccccct cttccagggg
     1441 tgcctttgta tccggtggag aataaggatt ggaactcatt atacgaatgc agtggttttt
     1501 cgataaaggt cttctggcat ttgttttctg agagtgtcaa ggattctgtc cttcgagtat
     1561 ctgggagaga aatgaatcaa gaccagatgc tctacttcct tgaaggcttc tgcattttcg
     1621 atgatttcaa aaagatgagt gtggccccac tttctcgctc tttccacggg ccgcgcttcg
     1681 tctatatagg tgcattccaa aaatagaatt ttggatcttc gaacgtcttc attttcaaga
     1741 gcgaattcta tctgtgtatc tccggagaaa gtaaccactg gcgattgaac gtcctcaaaa
     1801 agattcattg tttttttcat ttccgcaatc tgggggccgg tgaggtgact gtattctttc
     1861 cttagttttc gcactctctc gtagatggta tatccattgg atgcaactct gtgaacgctt
     1921 ctcaccgcac gaaacgaaaa gttgccgaag agaggataaa acgtggagta atccacgccc
     1981 tgcaggctga attccatctg gtagtcttcg atttcggacc agatgcgcaa gattctgcga
     2041 agtggatcct ctagctctgg agggcagtag atggttcctc caggaagatg acggaggcat
     2101 ctctgcgata cgtagtacgg aataccggat gcatgatcca ggtggccatg agttagcagc
     2161 acggttggaa tttcgacgag ttgattctgg catctccctg catcaaatgc aagatggaag
     2221 gtgggcagaa tcatactcgt ttccactcca ccgctggaga ttcccaggaa acgctgattg
     2281 cgtattgtgt attctctgag gctcaatgcg tgctcaggga agcttgcctt ccggtgtaac
     2341 catcacaacg gggcgaattt tttgcagctc aggtgcgagg cttttcgggc caacaaccac
     2401 aatcgtcatg tcagagtgaa cgtgtcgcgc gtatgcgcgg ctaacgtctt ctgcagtgag
     2461 agcgcgaatg tggtcagcgt agcgagcgag gtaatcgtca ggcatcccgt gcaacttgaa
     2521 gcgaacctgg ctttgtaaaa aactttcagg atcatcatat agaaatacaa gtgaattcag
     2581 aatgctttct cgcgccagat tcagttccac ggcatccgcc ggttgtctca tgccgccaat
     2641 gatttcgagc atgagcttta cagtctcatt cgcctgctca gagcgagtgc cggatgcagc
     2701 agtcagcctg ccatcatcct tgaagaacgt agtgcgggac atggagtagt atgccagtcc
     2761 gcgtgcggtg cgcacctctt tcatcaaacg gctgttaaac gaattgccac ccaggatatg
     2821 attggccact tgcagcgcat aaaaatcctc agacgagtgt ccgggcaaga tagtccccat
     2881 ggcaacggat gcctgaggaa cttctgcggt cacaagcagg attttgccgg caagaagtgg
     2941 agcgcccgcc ggtttctgaa tttgttcctg cgcagacttt gccggaaact gtgccagaag
     3001 accattgacc atggattcga ctggaacaga actcacatcc cctgcgcacg caacgatcac
     3061 accaggagca tacaatctgc gattgagatc ttcgcgtatg agttgtggcg tgattttttt
     3121 tagatctgtt ttttgcagaa cataaccgcg tctgctgttc ggatacatca cttcgagcag
     3181 ccttcgtcct gcgatggatt caggtctgtt gtttctctgg tcaatgtctg tttccattcc
     3241 gtccaggatc acggccaggc gttcttccga cagctttggc ttcagtagaa tgtccgaaac
     3301 aatggcgaat gccttcggga aattccattt caatgcagtg atgcttacca cccaggattc
     3361 atgatctccg cgaatgctaa aacgagcgcc cagtcggctc atttcttctg cgatttcatc
     3421 tccggatctt gaaccggctc cgtccacagc gagcaagctt gtggctgcgt cccagagtcc
     3481 tgcatgatcc actttctcga gtgagttgcc tcctttgaag tgaagttcca cagtcaaaaa
     3541 gggcagggac ttttcttccg ttacaagcat cgtggcaccg gaaggaagag tggtctgccg
     3601 taccggaggc ggcgcaaatc gaatcggagt ggatggaatg tctgcgatga gcttctctgc
     3661 gtgttcgcgc gattccttgg aagaaagatt ggctggaggt cttgtctgtt gcgccatcag
     3721 tgcggcgggc aggagaaaga ggatcagaga tcttttcatt gttcttctcc ctttgcctgt
     3781 ttgagtcgag cagaatgcat tttgtctggt gtcatatatt ctatggctgc tttctttacg
     3841 tctgcaggcg caatggcgct cagtgctgta atcttagtga agagtgtttc ataatttccg
     3901 tataacaact cgtaatacga aaggttgtcc gcatattctg cgttggatct gagcccgtag
     3961 aggaagtccg cgaccatgga tgctttgacc cgatcgagtt catcctgggt gacgccgtcc
     4021 ttttgaacag aagcgatttc ttcgagtact cccttttgga catcatcgta ggatttgccc
     4081 ggggccggga ctgcctggat ggtcagtaga ttgggatgtc tttctcctgg atatcctgtg
     4141 aatgcggaaa cttcagccgc gcttctgtct ttcatcacaa gctttcgcac aagtctgctg
     4201 tcgcttctac ctgcaaggat gctcgataga acttcgagac tcaggctgtc tttagtcatg
     4261 ccggatggtt tgttccacgc cagaaacatc acaggggatc ctggcttttc tatttctgtt
     4321 cgtgctctgg agggagccac ggcaacagaa ggtctctccg ggatgtcttt tcttttcaaa
     4381 tgagagaagt atttacgcat caacgcttct gtttgcgcca ggtcaatgtc tcccaccagt
     4441 gcgatgacag tattgttggg ggcgtaatag gtgttgtaga atgatttcgc ctgaccaaaa
     4501 ttcaaatact gaatgacctg catgctcccg atgagcgagc gcccgtacgg atgcgatccg
     4561 taaatgtctt tgatgaattg ttccagaagc gcgccctgag ccacgttgtc cacgcgcagt
     4621 cttctttctt cgcgaacaac ttctctctcc gtgtaaaaat cacgaagcac agagttctcc
     4681 attctgtctg attcgagttt ggcccatatt tcaaggcgat tggcaggcaa ttccacctgg
     4741 tagtttgtga ggtcctttgt agtgtacgcg ttgtagccgc gttctccggc ctgcgcgtag
     4801 atgtaggaat cttcatccgg gatcatgtat ttgcgcgcca tctgctggag actcatcaag
     4861 cgttgcttgc gcttttggac ttccgccttt gcttccgcaa tgcgagtagt atctccactt
     4921 ttttctgctg catccagggc gcgtctggcg tcgtcgagtc tgtctgccca gacgttgctc
     4981 tgttcaatgt atttcttttc tttctcgaag tccagagtac ccactctctc tgttcctttg
     5041 aacagcatgt gttcgagcat atgcgcgatt cccgcgcttt cttctgtttc atctgctccg
     5101 cccgctttga atttcatgta gcaggcgacc gtgggcgaaa aaggccgtcg aaccatgatg
     5161 atgcgaagac cgttttccag agtcactctg cgcacatccg tgcgaaggct tttcatggtg
     5221 gagtccagtt cagattgcgc ggccagaggc agggcaaaga tggagagcaa aacaaatacg
     5281 atgcgggaag tcacaggcga tccttttgag ggagagttct ctgtaaagag ttgtttgacc
     5341 gccaatgcgc aagtagaaaa actggtccag caagtccggg ccagtagctc agttggttag
     5401 agcacttctc tgataaggaa ggggtcggta gttcg
//
LOCUS       DAOQJL010000229         5408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8766_length_5408_cov_4.506632, whole genome shotgun sequence.
ACCESSION   DAOQJL010000229 DAOQJL010000000
VERSION     DAOQJL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8766_length_5408_cov_4.506632"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLG78_10825"
     CDS             complement(<1..1215)
                     /locus_tag="PLG78_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE23655.1"
                     /translation="MLIIRNPATGAVIAQKPVDTASQIADKYRRARKAQPTWAQTPYQ
                     IRAAAILRFRDLLIKNADDLAQTLSAEMGKPLSQAKGEIRATPQRIDFFLKHTEEAMK
                     EELAAESGLTGGTREVITQEPLGVIANISAWNYPYFVGNNVFIPALLTGNTVLYKPSE
                     FATLSGIAIDSMMHEAGIPADAFITVIGGGAEGAEILKHPVNGVFFTGSYGTGKKIAE
                     SVAGRMIRIQLELGGKDPVYVCEDVDIEKAAESLAEGAFYNAGQSCCAVERIYVNEKI
                     YDTFLDAFTKNVKSYKCGDPMQDGTFIGPLTRAPQLDILDRQVQEARDKGARILAGGK
                     RMLGPGQYYEPTVIADANNSLAVMREESFGPVIGLQKVKDDEEALRLMNDTDYGLTAG
                     VYTANEDRAKRIL"
     gene            1266..2729
                     /locus_tag="PLG78_10830"
     CDS             1266..2729
                     /locus_tag="PLG78_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23656.1"
                     /translation="MSSSLSLSLAVQKSGRWSLHFVLANPAAKIQGHTMRSVVVVILG
                     LLLLAPEVRTQEQPSVDQVRQKAKLHFDRARALERSKEMYKFTSEEFGSLLGDYEAGL
                     EILVSEAEAHPDTDDARKLVSQAAGPLREYANHALRKYRYDWVFDYWDRMRKIKKDIP
                     IEAEFASAMALQAHGAFAEGKYDEAIRIFSDMAQISREGSYGKLVMASYHPEFYEKIV
                     RRAKVLETMQVKPEYTAKVFSLRVGLLRFKWKENGLNGEPPTEVSSSPLKLRKGDVEL
                     ADMAEESLRRTVLAMSDGKLDLVFDRLALIPPLDVVLPDGQKLPNGITQSPSTSGDDI
                     IDRAAGEVGDLLRERTKAADVVILFWWGTSPLTQHGGSRSFKTGSGKSWRGNINIGVQ
                     FYHYDPGLDYMNCGACPGPNGIGFYPHVYMHEFFHVVESRFGIQPVHGFTMPQKFPAW
                     KGSDEVDYYWWQIGQNIPSLKKPGRRGNWSDFSFRER"
     gene            complement(2726..4708)
                     /locus_tag="PLG78_10835"
     CDS             complement(2726..4708)
                     /locus_tag="PLG78_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE23657.1"
                     /translation="MILSRRIVILCFALAIACRPAGQTPGVRHGQIDLRGFNAEDAVI
                     PLDGDWEFYWDQTERARTEPPEFLPVPGSWVDRGVHPPAGKAVYRLRVLSNQTGRVFA
                     LKIYEFPEAYRLYINGEKVLENGKYAEDPAQSARTLVRPVVTFPLKETNDIIFEVVNL
                     NEDEPGPRRSILLGTERAIRGVQENQIITDMITCGVLLIMGLYHLALYYQRRKENGAF
                     VFGLYCLIMAFRLLVTEEHYLHKYFPGFPGWLEQRLDVMSFFILPPVIAGIFSNFFRR
                     DFHSNLLRFIVWPCATLMAIYCIFPNRLIFETYILLSLVIGIYLGYVLVLGVRRSRRG
                     ALLFLVGWLIYLGTAILDFLSFNNIVRTIYVSHIGFLFFVFSQAYFLSLRFNRALATA
                     EELSERLELRVRERTRELNHSLEIIQKDLAMAREVQESLLKRKGPGYPGLVIVEKYIP
                     FSEVGGDIYDIREIAPGKVRIFVADANGHGVQAALITVMIKSEYDSLNQAQLRPAEII
                     ESFNRSYTDSRRSYVSMFSCFILDLDFEHRTIEYSSGGHPAQMLLRAGEILRLKPGGP
                     MIGIKGNARFGHGVLPLLPGDRFLIFTDGIFEEFNDRKEIFGEERLWASVGALSHQGM
                     SELTVSLLAEVQSFLGNAKWDDDTTILGIEVRIPET"
     gene            5101..>5408
                     /locus_tag="PLG78_10840"
     CDS             5101..>5408
                     /locus_tag="PLG78_10840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE23658.1"
                     /translation="MALWRALHVRIDAEPHVFVDEIGEKIIGEENWRARADMAPEFSK
                     SMRASVTGRARFIEDIVEEKVNEGVTQYVILGAGIDTFAQRRGDLASRLQVFEVDQPG
                     P"
BASE COUNT         1247 a         1322 c         1481 g         1358 t
ORIGIN      
        1 taggattcgt tttgctctgt cttcgtttgc agtgtagact cccgcagtca ggccatagtc
       61 tgtgtcgttc atgagacgaa gtgcttcttc gtcgtctttg accttttgca gtccaatcac
      121 cggcccaaag ctttcttccc tcatcacagc gagtgaattg tttgcatccg cgatgacggt
      181 gggttcgtag tactgccctg gtccgagcat gcgttttccg cctgcgagaa tgcgcgcgcc
      241 tttgtctcgc gcttcctgga cttgtctgtc cagaatgtcg agctgcggag cacgcgtgag
      301 agggccaatg aaggttccgt cctgcattgg atctccgcat ttgtagcttt tcacgttctt
      361 tgtgaacgcg tccaggaacg tgtcgtagat tttttcattc acgtagattc tttccactgc
      421 gcaacagctc tggcccgcgt tgtagaacgc gccctctgcc agagattccg ctgctttttc
      481 tatatccaca tcttcgcata cgtaaacagg atctttcccg ccgagctcga gctggatgcg
      541 gatcattcta cctgcaacag actctgcgat cttctttccg gttccgtagg agcctgtgaa
      601 aaatacgcca ttgaccggat gcttgagaat ttcggcgcct tctgcacctc cgccaatcac
      661 agtgataaac gcatctgccg gaatacctgc ttcgtgcatc atggagtcaa tggcaatgcc
      721 gcttaacgtg gcaaattcag acggcttgta gagcactgtg ttgcccgtaa gaagtgccgg
      781 gataaacaca ttgtttccta caaagtacgg ataattccag gcagaaatat ttgcgatcac
      841 gccgagcggt tcctgggtga tcacttccct ggtcccgcct gtgaggccgc tttctgctgc
      901 cagttcttct ttcatggcct cttctgtgtg tttgagaaag aaatcaattc tctggggagt
      961 cgcgcggatt tcgccttttg cctgcgaaag tggctttccc atctctgcgg aaagagtctg
     1021 agctagatcg tctgcgtttt tgatgagcag atcgcggaac ctcaatatgg ctgccgctcg
     1081 gatctggtaa ggagtctgtg cccatgtggg ctgtgctttt cgcgctctcc tgtatttgtc
     1141 tgcgatttga gaggctgtgt ctactggctt ctgggcaatc actgcgcccg tggctggatt
     1201 ccttatgatg agcattctgc actgattgca gatccgggcc acgcaatcat tcactttttg
     1261 gagtcatgtc gagctcccta tccctgagtt tggcggttca gaagtctggt agatggtcgc
     1321 tccatttcgt ccttgcaaat ccagcagcaa agatccaggg tcacacgatg cgaagcgtgg
     1381 tcgttgtcat tctgggtcta ctgctccttg caccagaggt gaggacgcag gaacaacctt
     1441 ccgtggatca ggttcgccag aaggcgaagc ttcattttga tcgagcgaga gctctggaaa
     1501 gatccaaaga aatgtacaaa ttcacgtcgg aggaatttgg aagcctgctc ggcgactatg
     1561 aagccggtct tgagattctc gtatctgaag cagaggcgca tcctgacaca gacgatgcac
     1621 gaaagcttgt gtcacaggcc gcgggacctt tgcgagagta tgcgaatcac gctcttcgca
     1681 agtatagata tgattgggta tttgactact gggatcgaat gcgaaagatc aagaaagaca
     1741 ttccgattga ggcggagttt gcatcagcta tggcgctgca ggctcatgga gcattcgcag
     1801 aaggaaaata tgatgaggcg attcgcattt tctcagacat ggcgcagata tcgcgtgaag
     1861 gatcgtatgg aaagcttgtg atggcatcgt atcatccgga attctacgaa aagattgtgc
     1921 gtcgtgcgaa ggtcctggaa accatgcaag tcaagccgga gtataccgcg aaagtttttt
     1981 ccctcagagt tgggctgttg cggtttaaat ggaaggaaaa cggcctgaac ggcgagccac
     2041 ccacagaagt cagcagttct cctctgaagt tgcggaaagg agatgtggag cttgcagata
     2101 tggcggaaga gtctcttcgc agaacggttc ttgcaatgag cgatgggaaa ctcgatctgg
     2161 tttttgatcg tcttgcattg attccacctt tagacgtggt gctgccagat ggccagaagc
     2221 ttccgaatgg aattacgcaa agcccttcca cgtcaggaga cgacatcatt gatcgcgccg
     2281 cgggggaagt gggcgatctc ctgcgcgaaa gaacaaaagc ggctgacgtt gttattttgt
     2341 tctggtgggg aaccagtccg ctcactcagc acggtggaag caggtctttc aagactggtt
     2401 caggcaaaag ctggagggga aatatcaaca ttggagtgca attctaccac tatgatcccg
     2461 ggctggatta catgaattgc ggcgcctgtc ctggacccaa cgggatcggc ttttatcccc
     2521 atgtgtacat gcacgagttt tttcacgtag ttgaatcgcg ttttggaatt cagccggttc
     2581 acggctttac tatgcctcag aaattccccg cgtggaaggg aagcgatgaa gtggactact
     2641 actggtggca aatcggtcag aacattcctt cattgaaaaa gcctgggcgt cgtggtaact
     2701 ggagcgattt cagtttcagg gagcgttagg tttccgggat gcgcacttcg attccaagga
     2761 tcgttgtatc atcgtcccac ttcgcgtttc caagaaaact ttgcacttct gccagaaggg
     2821 aaacagtcag ctcggacatt ccctgatggg acagagctcc cacagaggcc cagagccttt
     2881 cttcgccaaa gatttctttg cggtcgttga attcctcgaa aatgccgtct gtgaaaatca
     2941 agaagcgatc cccgggtaga agcggcagca cgccgtggcc aaatcttgcg ttgcctttga
     3001 ttccaatcat tgggccgcca ggtttcaggc gaagaatttc tccggcccgt aaaagcatct
     3061 gagctggatg tccgccggaa gagtactcaa tggttctgtg ttcaaagtcc agatcgagga
     3121 tgaagcagga gaacatggat acgtaggatc gtctactgtc cgtataactt ctgttaaacg
     3181 attcgatgat ttccgcgggg cgcaattgtg cctggttgag gctgtcgtat tcacttttga
     3241 tcatgaccgt gataagggcc gcctgcactc cgtgtccgtt tgcatccgcg acgaatatgc
     3301 gaaccttgcc cggagcaatc tctcgtatgt cgtagatatc cccgcccacc tcagaaaatg
     3361 gtatatattt ctctactatt acgagacccg gatatcctgg acctttgcgt ttcaggaggc
     3421 tttcctggac ttctcgcgcc atggccagat ctttctgaat gatttccagg gaatgattga
     3481 gttctctcgt gcgttctctg actcgtagct cgaggcgttc gctcaattct tccgcagttg
     3541 caagtgctct gttgaatctc agagaaagga agtaagcctg tgaaaagaca aagaacaaga
     3601 atccaatgtg acttacgtaa atcgtgcgaa cgatgttgtt gaaagaaaga aagtcaagaa
     3661 tcgcagttcc aagataaatc agccagccta ccaggaacaa aagcgcacct cttctcgatc
     3721 gccgcactcc gagcaccagg acatatccca ggtagattcc aatgacgagc gaaagcagga
     3781 tgtaagtctc aaagatgagt cgatttggga atatacaata gatggccatg agagtggcgc
     3841 aaggccagac gataaagcga agcaagttcg agtgaaagtc tcttctaaag aagttagaaa
     3901 agattcctgc gatgacgggt gggaggatga agaaagacat tacatcgagc ctttgctcca
     3961 gccatcccgg aaatccgggg aaatatttgt gaaggtagtg ctcctctgtc accagaagtc
     4021 gaaaggccat gatgagacag tagagcccga acacgaacgc gccattctcc tttcttcttt
     4081 gatagtagag cgcaagatgg tacaggccca tgatgaggag tacaccacac gtgatcatgt
     4141 ctgtgatgat ttgattttcc tggacgccgc gaatcgcgcg ctccgttcca agcagaatgg
     4201 atctgcgcgg tccaggttcg tcttcgttta gatttactac ttcgaagatg atgtcgttcg
     4261 tttcttttag agggaaggtg accacaggcc ggacgagtgt gcgggcactc tgcgccggat
     4321 cttccgcgta ctttccattt tcgagaactt tctctccatt gatgtagagg cgatatgcct
     4381 ccgggaactc atagatttta agagcgaaca ctctgccagt ctggttggaa agaacgcgca
     4441 ggcgatagac tgcttttccg gccggcgggt gcactccgcg atccacccag gatccgggca
     4501 ccggtaggaa ctccggcggt tctgttcgag cgcgttctgt ctggtcccag taaaattccc
     4561 agtctccatc gagagggatg actgcatctt ctgcgttgaa cccacgcaag tcgatctgtc
     4621 cgtgccgaac tccaggagtc tggcctgcag ggcgacaggc aattgcaagc gcaaagcaga
     4681 gaattacgat gcgccgggac agaatcacgg ttcagacact gggcaacagc ttttgtggag
     4741 caactcaaaa agtaagccct cgaatgcgcc tgcgggcagc ccctggcctg cgccagacga
     4801 gatggcaacc ctgatcgcag tcaaaggggg gcgtgcgtaa atgtgacctt tcgcttgctt
     4861 cattttgggc agatcgcaag gcacgttagt cgcttttgtt ggccacacgt ccacccatcc
     4921 tcgcgtctgc gcgtcgcccg cgcatagccc gcgcaaccag gtcgttgttt tatcacgcat
     4981 aaaaagtgcg tgttagtacg cggacgtgta gttattgcgt cctttgattg cgggataaat
     5041 caaaggcggt attggatggg caaatcacag acagcagctc cggaacatac agcggtgagg
     5101 gtggcattat ggcgtgcatt gcatgtgcga atcgatgcgg agccgcacgt ttttgtagat
     5161 gagatcggtg agaaaatcat cggcgaagaa aactggaggg cgcgcgcgga tatggccccg
     5221 gagttttcga agagcatgcg tgcttctgtg accggtcgag ctcgtttcat tgaagatata
     5281 gtagaagaga aggtgaatga gggcgtgacg cagtatgtga tccttggtgc gggcattgat
     5341 acctttgcgc agcgcagagg agacctggca tcgcgcctgc aagtgtttga agtcgatcag
     5401 cccggtcc
//
LOCUS       DAOQJL010000230         5394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8806_length_5394_cov_4.398577, whole genome shotgun sequence.
ACCESSION   DAOQJL010000230 DAOQJL010000000
VERSION     DAOQJL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8806_length_5394_cov_4.398577"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="PLG78_10845"
     CDS             <1..2460
                     /locus_tag="PLG78_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23659.1"
                     /translation="EESQKGKGRLHDPLTHMIVRTAVLEDRFPGAGEAMSGSHRWPHG
                     QTYYLYGAMMAEYLHKTEPEKAGKFWREETPVWFLEMKLSSMRIRSFNLLYRDFRTSK
                     EEEMRKEIESLRAAGLTQYKLLTQDGETKEHLTEGETGLLYFARPGARASGMFRLIPG
                     SEAERIRLSYTSEGMTERNGQILASEDHFFYPGLGIRQELHDARSVLFSRLLPGVSVV
                     DPLFSPDGNTLYYIEKDRFERRLIRASWKNEEIKDPETIFHVPFSGILRFPAVSPDGQ
                     QLAFVARKDETGMGVVLVCDARASNCKTVAAGPAAKVRPRFSKDGKQLFFSSDADGIY
                     NIYSVNLASGEILKRTRTLTGFFDPVPTESALYALSYNGKGFDVAEISYEHMLAEKSG
                     LFAAHGDESSFFEAETEAPLPGQPQNWPEDPDYNGALSMRPYFAGLLGSGSLLGLGVA
                     ALDPLERHVLAAGIVAGAPDPYAWAFYDYSRFVVGFSGFYSTNYWKRDRAPGCLNPSD
                     PNRFICDDQYSFVESMGGYLRYTFEGRYVSFQVLPGYASTKMRNARRIRTLEMDARDL
                     NLSGPSFAMLLGRTEEFAESVSPERGFRFFALTDYYSKTTSRRELDPQTQRPIDYGAA
                     EGGLALYLPSFFDHHVNYLSGYAFTSYGPDREVEKVRLSRLQRGIAYDRSPTATSAVV
                     WTYEYRLPIAWLSASPESMPHFILRNIGMSLFYEAGAAFEKIPYQRDYKSSVGVTFQF
                     GITALYLPLAPFRLTFVKGLGREGENQVYLGYETTFGPGMDSGRNIPGNRIVEPWHRP
                     IPRSTEEPGYFRRASAGGILE"
     gene            complement(2469..3536)
                     /locus_tag="PLG78_10850"
     CDS             complement(2469..3536)
                     /locus_tag="PLG78_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23660.1"
                     /translation="MAESLLKKQRPAKTPKQRVFVAALPGESDLPAFLEGLYATRLIR
                     MRHPDIRTMLIVAPANRALAEECGLFDEIIDAAGDLSTLIRNVHADIIYVPSVDFRLS
                     MKLLFSGSIRIAGGARLGKFLRFFDLRRDGEKLKKAGLDMLPESVGISFRNAPLPDEM
                     IVYASLFDEHNVSGSWPVGHAARLSRLLSTLGAKMIVPLPEEKFTRTFTPLPSSNSRE
                     FAKDVQYLKKYAPEIQFVSAKEIQKKAEWMRKSSAIVAPAGSETVFAALLHRPVVTLH
                     DMQSHRMLGKPDARSLGPRTADPGVLGLFTKIADSLDRHLMPQVEECIEDCPACTHLS
                     CVDTISPERVFENVKRILMPY"
     gene            complement(3529..4170)
                     /locus_tag="PLG78_10855"
     CDS             complement(3529..4170)
                     /locus_tag="PLG78_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE23661.1"
                     /translation="MPIGHPSDITLRALESFRDANLIFVEDPSTVSRLFKKWDVPFIR
                     ERIRVLNEHTTADELNDLVREAANVELSVVVSDAGMPVFCDPGGDFMRLCEKAGIRIE
                     IVPGPTALTSALVRAGINGAFYFAGFPPRKSEERGGFFRKLNQMTVPIVLYETPYRLE
                     KILGEIQRHFRPDKKVIVCAGLTSPDESIVTSTVANAGKIRIQSAPPVIIIDG"
     gene            complement(4194..4736)
                     /locus_tag="PLG78_10860"
     CDS             complement(4194..4736)
                     /locus_tag="PLG78_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE23662.1"
                     /translation="MIAEKQINQQMQMQDIMNIYPSAKRALFQKYHIGGCSSCGFAPT
                     DTLAEVFVKHNRPAEVDGAIEWIYESARVDEAMQIDPQTLKSEMDAGTPWKLIDVRDE
                     MEAEIVSIPGSELITREMAYEILQKWPKDTKIAFYCHTGVRSLEAASYFKGHGLPNVK
                     SLRGGVDRWAVEIDTSLPRY"
     gene            4809..5297
                     /locus_tag="PLG78_10865"
     CDS             4809..5297
                     /locus_tag="PLG78_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23663.1"
                     /translation="MKIWLPVLFFLTAACLGSIRDKHKPRVVLSWHEKVNRAGVMIRD
                     LGLDVLAEPPGSLPYSSNASAAVWLGVNFPVDQLADVMDIGRTYYPDLRYVALSDYGL
                     DSPPAQVHDEVYIGGSTETALKLGLKAWTDQDFRKLRTLKTKEEVAAFVRSKYGPRRS
                     LK"
BASE COUNT         1333 a         1395 c         1367 g         1299 t
ORIGIN      
        1 gaagaaagtc agaaagggaa aggtagactc cacgatccgc tgactcatat gattgttcgc
       61 acggcagtac tcgaagaccg gttcccgggt gctggcgaag caatgtctgg ctcacacaga
      121 tggccgcatg ggcaaactta ctacctctac ggcgccatga tggcggaata tcttcataag
      181 accgaaccag aaaaagccgg aaaattttgg agagaggaaa cacctgtctg gtttctggaa
      241 atgaaactct cctcgatgcg tattcgctct ttcaacctgc tctaccgtga ttttcgcact
      301 tcaaaagaag aagagatgcg caaagaaatt gaatcgctca gggcagcagg tcttacgcag
      361 tacaaactat taacgcagga cggagaaaca aaggaacacc tgacagaagg agaaacggga
      421 cttctgtact tcgcgcgtcc cggcgctcgt gcatccggca tgtttcgatt gattccgggg
      481 tcagaagcgg agagaatccg tctttcctat acgtcggagg gaatgacgga aagaaacggc
      541 cagattctcg caagcgaaga ccatttcttt taccccgggc ttggaatcag acaggagctt
      601 catgacgcca gaagtgttct gttttcgcga ctcctccccg gcgtgagcgt agtggatccc
      661 cttttctctc cagacggaaa tactttgtac tacatagaaa aggatcgctt tgaaaggcga
      721 ctgatccgcg ccagctggaa aaatgaagaa ataaaggatc cagaaaccat atttcatgtt
      781 cctttttctg gtattctgcg tttccctgcg gtttctccgg atgggcagca acttgctttt
      841 gtggccagaa aagacgaaac agggatggga gtggtgcttg tatgcgatgc aagggcatcc
      901 aactgcaaga ctgtggccgc cggtcccgca gcaaaagttc gccccagatt ttctaaggat
      961 ggaaaacaac ttttcttctc ttcagatgct gacggaatct ataacatcta ctccgtaaac
     1021 ctcgcgtcag gagaaattct gaaacgcact cgcacactca ctggcttctt tgatccagtg
     1081 cctaccgaat ctgcgctgta tgcattgtcg tataacggta aggggtttga tgttgccgag
     1141 atttcgtacg aacacatgct tgcggaaaaa tctggattgt ttgccgctca tggagacgaa
     1201 tcgtcattct ttgaagcaga aacagaagca cctctacccg gacaaccgca gaactggccg
     1261 gaagatccgg attacaacgg agcactttcc atgcgccctt acttcgccgg cttgcttgga
     1321 agcggcagcc tactggggct cggggtggcg gcgcttgatc cgctcgagcg gcatgttctg
     1381 gcagcaggta ttgtggccgg tgcgcctgac ccgtatgcct gggccttcta cgattactca
     1441 cggttcgttg taggattttc cggcttttat tctacaaact attggaagag agatcgcgcg
     1501 cccggatgct tgaatccatc agatccaaat cgtttcattt gtgacgatca gtattctttt
     1561 gtcgagagta tgggaggata cctgcgatat actttcgagg gcaggtatgt ttccttccag
     1621 gtcctacctg gatatgcatc caccaaaatg cgaaacgcgc gaaggattcg gacgttggaa
     1681 atggacgcgc gtgacttgaa tctatccggt ccttcatttg ctatgcttct gggcagaaca
     1741 gaagagtttg cagaatcagt gagcccggaa aggggctttc gcttttttgc actcacagat
     1801 tactactcca aaacaacttc gcgcagagaa ctcgatccgc aaacacagcg acccattgac
     1861 tacggtgcag cggagggcgg gcttgccctc tatcttccgt ctttctttga tcatcacgta
     1921 aactatctta gcggttatgc attcacatct tacgggccag atcgcgaggt agagaaagtc
     1981 agactgagca gactgcaacg cgggatcgcc tatgaccgct ccccgaccgc gacgagcgcc
     2041 gttgtatgga catatgaata tcgtctacca attgcatggc tttctgcgtc tcctgaatct
     2101 atgccgcact tcatactacg caatattgga atgtctctgt tttatgaggc gggcgccgcc
     2161 tttgagaaaa ttccatacca gagggactac aaaagcagcg tgggcgtgac cttccagttt
     2221 ggaatcacag cactctacct tcctctcgct ccattccgcc taacgttcgt caaaggtctt
     2281 ggccgcgagg gagaaaacca ggtctatctt ggttacgaaa caacgtttgg tcctggtatg
     2341 gacagcggac gcaatattcc ggggaatcga attgtggaac cctggcatag accaattccg
     2401 cgaagcacag aagaaccagg atatttccgc cgcgccagcg ccggcggcat actcgaatag
     2461 ctagcgtctc agtaaggcat caaaattcgt tttacgtttt cgaaaactct ttccggcgaa
     2521 attgtatcca cgcaggatag atgcgtgcag gccgggcaat cttcaatgca ttcctctact
     2581 tgaggcatga gatgcctgtc cagagagtct gcgatttttg taaacaaacc cagcactcct
     2641 ggatccgcag ttctcgggcc cagagatctg gcgtcgggtt tgccgagcat tctatgcgat
     2701 tgcatgtcgt gtaacgttac aaccgggcga tgcaaaagtg ctgcaaacac agtctcggac
     2761 ccggccggcg ccacaatcgc agaagatttt ctcatccatt ctgctttctt ttgaatttcc
     2821 ttcgcagata cgaactgaat ttcgggtgca tacttcttga gatactgaac gtcttttgca
     2881 aactcacggc tgttcgaaga agggagcgga gtaaatgttc gagtgaattt ttcttccggg
     2941 agtggaacga tcatcttcgc cccgagcgta gaaagcaggc gagaaaggcg cgctgcatga
     3001 cccacaggcc agctaccgga aacattgtgc tcatcaaaga ggctggcata cacaatcatc
     3061 tcgtcaggga gcggggcatt gcgaaatgaa attccaacgg attccggaag catgtccagc
     3121 ccggccttct ttaacttttc gccgtcgcgg cgaagatcaa agaaacgcag gaattttcca
     3181 aggcgagcgc ctcctgcaat gcgaatggat ccggaaaaca gcagcttcat gctcaagcga
     3241 aaatcaaccg aaggaacata gataatgtcc gcgtgaacgt tccgtatcaa agtggacaga
     3301 tcccccgcgg catcgatgat ctcatcaaat agcccgcatt cttctgcaag tgcacgattc
     3361 gcaggagcaa caatgagcat cgtgcgaatg tcaggatgac gcatgcgtat cagtcgagtg
     3421 gcgtagagcc cttctagaaa cgcaggtagg tcgctttcgc ccgggagcgc cgccacgaac
     3481 actctctgct ttggtgtttt agccggacgc tgttttttta gaagagattc agccatcaat
     3541 gatgattacc ggaggtgctg actggatgcg aatcttaccc gcatttgcta ctgtcgaggt
     3601 gacgatactt tcatctggcg atgtcaaacc ggcgcagaca atcacttttt tatccggcct
     3661 gaagtgtctc tggatttcac cgaggatctt ttccagacga tacggagttt cgtacaatac
     3721 gattggtacc gtcatctgat tcagttttcg gaagaatccg cctctctcct ctgattttct
     3781 tggagggaag cctgcgaagt agaaagctcc attgattccc gctcggacaa gagcgcttgt
     3841 cagagccgta ggaccaggaa cgatttcgat tcgaatgccc gccttttcgc acaggcgcat
     3901 gaagtctccg cccggatcac aaaacacggg catgcccgcg tctgaaacaa ccacagagag
     3961 ttctacgttt gccgcttccc ggaccaggtc atttaattca tccgctgtgg tgtgttcgtt
     4021 gagtacgcga atgcgttcgc gaatgaaagg cacgtcccat tttttgaaaa gacgcgaaac
     4081 tgtagaagga tcttcgacga agatcaaatt cgcatcacga aaggactcaa gagcgcgaag
     4141 ggtaatgtca gagggatgcc cgatgggcat ccctgtaact ctaagtgtcg agatcagtat
     4201 ctaggaaggc ttgtatcgat ctcaaccgcc cagcgatcta ctccaccgcg cagactcttg
     4261 acattgggaa gtccatgtcc tttgaaatac gacgcagctt ccagagagcg aactccagta
     4321 tgacagtaga atgcaatctt tgtatccttt ggccactttt gaagaatctc gtacgccatt
     4381 tcgcgcgtaa tgagctctga gccgggaatg gatacgattt cggcttccat ttcatcgcgc
     4441 acatcaatca gcttccacgg agttcctgca tccatctcgg acttcagagt ctgaggatca
     4501 atctgcatgg cttcgtccac acgagcgctt tcatagatcc actcaatggc gccatccact
     4561 tctgcagggc gattgtgttt cacaaaaacc tctgccagag tgtccgtagg cgcaaacccg
     4621 cagctagagc atccgccaat gtggtacttc tgaaacaaag cacgcttggc gctcggatag
     4681 atgttcataa tgtcttgcat ttgcatttgc tggttgattt gcttttcagc gatcatagtc
     4741 cctccctcag ggcggaaaat ttagatttgc gtttatggac ggccctgaat gactacgcgt
     4801 caagtataat gaaaatctgg ttgcctgtcc tctttttcct gacagccgct tgtctgggct
     4861 ctatccgaga caagcacaag cccagagtag tcctcagctg gcatgaaaag gtcaaccggg
     4921 ccggtgtcat gatccgagac ctgggtctgg acgttctggc agaaccacca ggatcgcttc
     4981 cctactccag caatgccagc gcggccgtgt ggctgggtgt gaactttcca gtggatcaac
     5041 tagcagatgt tatggacata ggtaggacct attacccgga cctcagatac gttgcacttt
     5101 cagactacgg cctggacagc ccaccggccc aggtgcacga cgaggtctac atcggtggtt
     5161 ccacagagac tgctctcaaa ctcggactca aagcctggac ggaccaggac ttccgcaaac
     5221 tgcgcaccct gaaaacaaag gaagaagtag ccgcctttgt cagaagcaaa tacggcccgc
     5281 ggcgttcctt gaaatgaatg ttccggacat tgactcactg gaactgattc ctcacttcca
     5341 gcctatcatc cgactggacg atcgtgccat ctgcggctat gaatccctgg gaag
//
LOCUS       DAOQJL010000231         5372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8859_length_5372_cov_3.938123, whole genome shotgun sequence.
ACCESSION   DAOQJL010000231 DAOQJL010000000
VERSION     DAOQJL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8859_length_5372_cov_3.938123"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLG78_10870"
     CDS             complement(<1..1055)
                     /locus_tag="PLG78_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23664.1"
                     /translation="MIFLTAHTTANIILVIFNLLVGGFLILRPGRTTASFHLGLGFIF
                     MAVFPSGYVFGTSLYHPIGAFHRYMTVSFVVPLCMQFGQFFLRYPANEHRRGANALLA
                     VGWIVSASLSIYFILSTFYGPVEFEFSSGVFNFVADRPTRIVGLFILAAVVHIIFVLG
                     YRILRKDPESKFLTALAALLIGSSFPTAIVNYLVTLGYFDRGFFFNMFIFNILTFFFG
                     FVILYINHTHERSTFMAKLVGITVVTVLLLVQSFSYYTFGKAERSFDETMLEQSIRLA
                     SDPRQTGAASYVATMEPLGNLRWSSLAASPDIEMTKNAIANARWIETLRQKTAAPAER
                     PDSFLLYDAILAERRKA"
     gene            complement(1130..2518)
                     /locus_tag="PLG78_10875"
     CDS             complement(1130..2518)
                     /locus_tag="PLG78_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE23665.1"
                     /translation="MNELFSESTELDRKLGLKETSAIVVGRIIGSGIFRTPSSIFALV
                     GCSSLFYGVWILGGLVSLMGAFCVMEMIAMMPRSGGPYAFLKAAYPPVWTFLRGWAMF
                     FVAETGAIAAVALVFAEYSEFLSKELGGPLFGIAGRTAIALMLVWSWTAANCFGVQLS
                     GRLQVVFSSAKVLALLSVIVAGIALRPDPDHIANNFWPQNGGIGTVLAFFAAMRYGFF
                     AYSGWEGATYVAEEVKNPRRNLPLSLLLGISGVMLLYLAANTGYLLQLTGSEMHAARQ
                     YIAADAMRKALGAIGGVLIAAAVMINTFGNVGTQILVKARTWFAMARDGLFFPRLGEL
                     SAIYHTPNRALIAQAFWASVLLCFASLAKNAYETVIDFFAFTGAFFNVLTFLALFVLR
                     KKFPDAPRPFKMPLYPLPALIAIAVYVMLMISALFTAPLESLSGVALTASGLLYYRYL
                     RKGARSDLLQNH"
     gene            complement(2515..5079)
                     /locus_tag="PLG78_10880"
     CDS             complement(2515..5079)
                     /locus_tag="PLG78_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNE23666.1"
                     /translation="MNSNNGSLQSTNNSTKVMTGFRKAAPGAVFTIAVLSFSTLGAQE
                     SFNPASVMPAECRNGSYHCGFLPPNPDEYRSLPMVDSAFYKERGLPSSVDMSADMPPV
                     GFQGDQGSCLGWSLAYAIRSYQEKKAFGWSYDSPIYGGQGTRVMSPSFIYNQINGGRD
                     QGSDPIAALRLLVTRGVSTWKRMPYSLGDYRSQPGPEALAEAMQFRSRGFRAIDSAAV
                     DSIRTELSKGRPVMLGILAYENFYKLDKSNPVYDDFRGKFFGGHAVTIVGYDDSKDTP
                     GGRGAFKVMNSWGRAWGENGFAYISYRVFPKIVTAGFVIEQRDKASASPPSLEHLLLA
                     PREVAATRGVFEDKVELTWTSADGAVAYEIQRSSMRFERIGFSVAPRFTDNNVEKGIP
                     YKYRIIAIYRTGRSDPELSPTAEGFARNPGIFERYVLPPVLDIVANSLGTAFRSLVTP
                     DALPSQMTQTPAAASTNGITLSWNQTSGAEWYDIARYEPALKSFQLLAVSRETTFRDP
                     AGRMDAVYSIRARSRLTSGAWSAPIMAGNGVTRLDASRGLYRDRITVKWDPVPGANGY
                     IIHFYDATKQKYTGSTKTTSNQYTDESERAKSGSWTGYTVAALFEDQTGKFTGPVFGK
                     SNPALNRMILPPPSGLAVEVTRDGAQIQWNQVAGAERYFVFRKRERDSDFAMYAEVRA
                     NQFRDKELKAGELYYYTVRASNEEGESLDSQSLAAALPGNLLALVPQQPAPPNAGQRA
                     SRVSEDSARTESSKISQNIGAGDPFARNWKGSYWDGKELSPVSVKVNMKGEIAEAVVQ
                     FAGRPAQIIKGIHPAGAQIFEIPGIKATLSRDALMLRFTDNNIAPYPIQVAMDPSP"
     gene            complement(5042..>5372)
                     /locus_tag="PLG78_10885"
     CDS             complement(5042..>5372)
                     /locus_tag="PLG78_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNE23667.1"
                     /translation="IFMVRDGVLVTPPVAASVLEGVTRRSVLELAKSEGIPVEERDIA
                     RAELYVADEVFFTGTGAQIAWVSSIDHRVIGTEVGPVTNKLRSRFLKCVLGEDSEFKQ
                     WVTPVYE"
BASE COUNT         1386 a         1354 c         1384 g         1248 t
ORIGIN      
        1 ttggcctttc gcctttcggc cagtatggca tcatacaata gaaacgaatc aggcctttcg
       61 gctggcgcag cggttttttg ccggagggtt tctatccagc gtgcattagc aatcgcattc
      121 ttcgtcattt caatatcggg agatgcggcc aaactcgacc agcgcagatt tccgagcggt
      181 tccatggttg caacatagct ggccgcacct gtctgacgtg gatccgaagc aaggcgtatg
      241 ctttgctcga gcattgtctc atcaaaggat ctttccgctt ttccgaatgt gtagtagctg
      301 aaactttgaa caaggagcaa cacagttacg actgtgattc ccaccagttt ggccatgaaa
      361 gtagaacgtt cgtgcgtgtg attgatgtag aggatcacaa atccaaaaaa gaaagttagg
      421 atattgaaga tgaacatgtt gaagaaaaag ccgcggtcga aatatccgag cgttaccaga
      481 tagttgacaa tggcagtcgg gaatgatgat ccaattaaaa gggccgcgag cgccgtcagg
      541 aattttgact caggatcctt tctcaggatg cgatatccga gtacgaaaat aatatgaact
      601 acggctgcga ggatgaacag acctacgatc cgggtgggtc gatcggcgac gaagttgaac
      661 acgccggaag agaattcgaa ctcaacggga ccgtaaaaag ttgagagaat aaaatagata
      721 ctcaggcttg cggaaacgat ccagccgact gcaagcagtg cattggcgcc gcgcctgtgt
      781 tcgtttgcgg ggtatctcag aaagaattga ccgaactgca tgcacagtgg caccacaaaa
      841 ctaactgtca tatatcgatg aaatgccccg atcggatgat agagcgaggt tccaaaaaca
      901 tagccggatg ggaacacggc catgaaaatg aaaccaaggc ccaaatgaaa cgacgcagtc
      961 gtccgaccgg gccgaaggat gaggaatcct cccaccagta gattaaagat cacaagaatg
     1021 atattggcag tcgtatgcgc agtcaggaag atcatgggac gctacggtaa gtccgatgga
     1081 atctaattgt caagcattcg aactttcctg tcagacaaca ggatgaaatt caatgatttt
     1141 gaagcaggtc tgaacgcgcc cctttccgca aataacgata gtacagtaaa ccacttgcag
     1201 tcaaagccac tccgctgagt gattcgaggg gagcagtaaa caaagcggaa atcatgagca
     1261 tcacataaac agcaatggca atcaaagccg gaagcggata caaaggcatc ttaaaggggc
     1321 gaggagcatc cggaaacttt ttccgcagta cgaagagagc aagaaaagtc agtacattga
     1381 agaatgcacc ggtgaaagca aagaaatcaa ttacagtttc gtaagcgttc tttgcaagag
     1441 atgcaaaaca aagaagcacg ctggcccaga acgcctgagc aatcaatgcg cgattgggtg
     1501 tgtggtagat ggcactcagt tccccgaggc gcggaaaaaa aagaccgtca cgcgccattg
     1561 caaaccatgt gcgagctttc acaagtattt gtgtgcccac attgccaaag gtattgatca
     1621 tcacagcggc cgcgatcagc acccctccta ttgcacccag ggcctttctc attgcatccg
     1681 ccgcgatgta ctggcgagca gcatgcattt cacttccagt caattgtagc aaataaccag
     1741 tgttagctgc cagatacaag agcatcactc cggaaattcc gaggagaagg gagagaggaa
     1801 gattgcgtcg cggattcttc acttcttcgg caacataggt ggcgccctcc caaccgctgt
     1861 acgcgaagaa gccatatcgc atggctgcaa aaaacgcgag cacagttcca attcccccat
     1921 tttgcggcca gaagttgttc gcgatgtgat ccgggtcagg gcgaagagcg atgcctgcga
     1981 ctattacaga gaggagggca aggacttttg cggaactgaa cactacctgc aatctaccac
     2041 tgagctgcac gccaaaacaa tttgcggccg tccatgacca gacaagcatg agtgcaatgg
     2101 cagttctgcc cgcaatgccg aataacggtc ctcccagttc tttggaaaga aactctgagt
     2161 attcagcgaa cacaagtgct accgcggcga tagctccagt ttccgccacg aagaacatgg
     2221 cccagccgcg aagaaatgtc cagaccggag gataagccgc cttcaagaaa gcatagggcc
     2281 ctccggagcg cggcatcatt gcaatcattt ccatgacgca gaatgcaccc atcagagaca
     2341 caaggcctcc tagaatccac acgccgtaaa agagagaaga gcagccaacg agtgcgaaga
     2401 tggaggaggg cgtgcgaaag atccctgatc ctatgattct accaaccaca atggcggatg
     2461 tttctttgag tccgagtttg cgatccagtt ctgtggattc gctgaaaagt tcgttcacgg
     2521 agatggatcc atcgccacct ggatcgggta gggtgcaatg ttgttatcag taaatcttag
     2581 catgagcgca tctctgctga gcgttgcttt gattcccggg atttcgaaaa tctgcgcgcc
     2641 cgctggatga attcctttaa tgatttgtgc cggtcttccc gcaaactgaa ctacggcctc
     2701 tgcgatttca cctttcatat ttacttttac agacacaggc gagagttcct ttccatccca
     2761 gtaagatccc ttccaatttc gcgcaaacgg atcaccggcc cctatgttct ggctgatttt
     2821 tgagctttcc gttcttgcag aatcttctga tactcgagat gctctctgtc ccgcattcgg
     2881 aggagctggc tgttgcggaa cgagcgccag aagattgccc ggcaaagcgg cagcgagcga
     2941 ctgagaatcg agcgattcgc cttcctcatt gctggcacgg acagtatagt agtagagttc
     3001 tccggccttt agttccttgt ctctgaattg attggctcgc acttctgcgt acattgcaaa
     3061 atcagagtca cgctctcgct tgcggaaaac aaaataacgt tctgcgccgg ccacctggtt
     3121 ccactggatt tgtgctccgt ccctggtcac ttccaccgcg aggccgctcg gtggaggaag
     3181 aatcattcga ttgagcgccg ggtttgattt tccaaacact ggtcccgtaa attttccagt
     3241 ctgatcttcg aagagggcag caacagtata gccagtccac gaacctgatt tcgctcgttc
     3301 ggattcatct gtgtactgat tcgatgttgt cttcgtgctg ccagtgtatt tctgtttcgt
     3361 agcgtcatag aagtgaatga tgtagccgtt tgccccaggg accggatccc attttaccgt
     3421 tatgcgatca cggtacaacc cgcgagatgc atcgagcctg gttaccccgt ttcctgccat
     3481 gatcggagcg ctccaggcgc cggatgtcag gcgagatctg gctcgaatag agtatactgc
     3541 gtccattcgt ccagctggat cacgaaacgt agtttcgcgc gaaactgcca ggagttgaaa
     3601 cgatttgagt gctggctcat agcgcgcaat gtcgtaccac tctgcgccgg aagtctgatt
     3661 ccaggagaga gtgatgccat tggtagatgc agccgcaggt gtctgcgtca tttgggatgg
     3721 gagtgcatcc ggagtcacca aagaccgaaa ggcagtgccc agggagttcg ccacaatatc
     3781 gaggacgggt ggaagaacat aacgttcgaa gatgccaggg ttgcgcgcga agccttctgc
     3841 agttggactc agttcaggat cggatctgcc tgtgcgatag atggcaatga tcctatattt
     3901 gtaaggaata cctttttcta cgttgttgtc cgtgaaacga ggtgccacag aaaacccgat
     3961 tctctcaaat cgcatagaag atcgttgtat ttcataggcg actgcgccat ccgcactcgt
     4021 ccaggtgagt tccactttat cttcgaagac ccctcgcgtt gcggccactt cgcgcggagc
     4081 aagaaggaga tgttcgagag aaggcggact ggcgctagcc ttgtctcttt gttcaattac
     4141 gaatcctgca gtgacaattt taggaaacac tctgtagctt atgtaagcga atccgttttc
     4201 tccccacgct ctaccccatg agttcattac tttgaaagca ccccgcccgc caggtgtgtc
     4261 tttgctatca tcgtatccta cgatggtcac cgcatggcct ccgaagaact ttcctcggaa
     4321 gtcatcgtat acaggatttg atttatctaa tttataaaaa ttctcatatg ccagaattcc
     4381 aagcatcaca gggcgacctt ttgataattc tgtgcgaatc gaatctacag ccgctgaatc
     4441 gatcgcgcga aaaccgcgtg acctgaattg cattgcctcc gcgagagctt cgggccccgg
     4501 ttgcgaacga tagtcgccca gactgtacgg cattctcttc caggtggaca ctcctcgcgt
     4561 tacgagaagg cgaagcgccg cgattggatc agaaccctgg tctctcccgc cgttgatttg
     4621 attgtaaatg aaagacgggg acatgacgcg tgttccctgg cctccgtaaa tgggagaatc
     4681 atagctccaa ccgaatgctt tcttttcctg ataagatcga atcgcgtacg ccagagacca
     4741 tccaaggcag ctaccctgat caccctggaa tcctacaggt ggcatatctg ccgacatatc
     4801 cacggaagaa ggaagacctc tctctttgta gaaagcactg tccaccatgg gtagacttct
     4861 gtattcatcc ggatttggag ggaggaaacc gcaatgatat gaaccgtttc tgcattctgc
     4921 aggcatgacg ctggcagggt tgaatgattc ctgagcgcct aacgttgaaa aagaaagtac
     4981 ggcgatcgtg aagactgctc ccggagcagc cttgcgaaag ccggtcatca cctttgtgct
     5041 gttattcgta gactggagtg acccattgtt tgaattcact gtcctctccg agaacacact
     5101 tgaggaatct gctgcgaagt ttattggtaa caggtccgac ttccgtgcca atgactctgt
     5161 gatcaatgga ggatacccag gcgatttgcg cgccggtacc cgtgaagaag acttcatctg
     5221 cgacataaag ctctgcgcga gcaatgtctc gttcttccac cgggatgcct tctgatttcg
     5281 caagttccag cacgctgcgg cgtgtgacac cttcaaggac ggaggcggca acgggcgggg
     5341 tgacgagtac tccatcgcgc accatgaata tg
//
LOCUS       DAOQJL010000232         5351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8905_length_5351_cov_4.551926, whole genome shotgun sequence.
ACCESSION   DAOQJL010000232 DAOQJL010000000
VERSION     DAOQJL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_5351_cov_4.551926"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLG78_10890"
     CDS             complement(<1..1141)
                     /locus_tag="PLG78_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23668.1"
                     /translation="MRLSFLILLFPATALFSETILMQNGTYVRGNIIAQDRTSVTIDT
                     ATGRLSLQKATIKRIMYDDAKFDAEQKAREEGQRRQEAKNAEERRLAEEKRQAALREE
                     SKNKPDEPPGLQRTGPVLGVGLSTNRYSPGTIDYTKREKENPLGVSNLPSYPANFRTR
                     GYGGLRGSLGYQSRKWFILVDAMQIHGHSKYDELGGGYTFLQSPNPQLRLIDGRGAAN
                     STRTGIFSRAGFSPVPATWFIRPHLYAGYSSTNIQTRLVSTTLNYQPGVRNILLTAMR
                     ANVNGKGPELGGEIRFLRESGLELRTLYGQSWQRGHAKPVGNTIAFRAEPASSLLDNL
                     WIRSDGKGDSFLRVQRYAATMFSPRLGVGRAFFSLRAEHSVIHVHR"
     gene            complement(1227..2846)
                     /gene="nadB"
                     /locus_tag="PLG78_10895"
     CDS             complement(1227..2846)
                     /gene="nadB"
                     /locus_tag="PLG78_10895"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNE23669.1"
                     /translation="MTRIVDTDYLVIGSGISGLYSALILSEFGKVTIITKEEVTDANT
                     TYAQGGIASVLAASDTFESHIRDTLDAGAGLCDTEAVRVLVTEGPTHIRRLMELGAAF
                     DQDASGSLTLGREGGHSENRIVHAKDLTGKEVEDVLLRAVRARNIEIKEYQSAVDLIT
                     CYHLEKDPYLPGVAPPGPNQCFGAYVYDRKTWEIYIIRARATILATGGAGQVYLHNTN
                     PDVATGDGVALAYRAGAVISNMEFYQFHPTALYSPGHRTFLISEAMRGEGGILRGPDG
                     EPFTKKYDERGDLAPRDIVARAIDAEMKRTGAAFMHLDVTHLPREQIMKKFPNIYAHC
                     LDLGIEISRDRIPVVPAAHYMCGGVRTDVNGRTAIEHLFAVGEVSCTGVHGGNRLASN
                     SLLEGLVFADRIGRYIKENQISGQRPEIRRWQKEGLANAEEWILVQHNLGEIKSVMWD
                     YVGIVRSNLRLDRALRRIEMLSNEINDFYSRTVIQNKILELRNLALVGKLIVRSAKAR
                     RESRGLHFSTDYAENREPSMAYTSLQRSSAG"
     gene            complement(2830..3384)
                     /locus_tag="PLG78_10900"
     CDS             complement(2830..3384)
                     /locus_tag="PLG78_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNE23670.1"
                     /translation="MRSPRILRLAIVAAAVLIAGALLYEGGRYLWREKRWNWIVVHHT
                     ASDFGNLAYFRDLHQREKGWPDIAYHFLINNGTAGTAAGQVEESNLWHDRDGGYSTKV
                     HEVNQFGIAIVLVGNFEKHSVPDQQMEALLHLILRLKREHGIPLDHIVGHRELWATAC
                     PGKHLNMSEVRAKIAEMEKNDAHR"
     gene            3464..4684
                     /locus_tag="PLG78_10905"
     CDS             3464..4684
                     /locus_tag="PLG78_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23671.1"
                     /translation="MALTQRGKGWEWFQSWYMLLSLPFGFTTFLAFLYTGFRVKKWLW
                     MGFGVAYLLLNIYVVKKIPAADSGVPADDLTMGLLFGEWLVGMIHVIVIRKSYLLLLE
                     ATEQSSKISDDRLRTKIQADYGVSENKIDAALVEFKETDATVRVVSMLFSVFPFIPQY
                     EYYFSLKGAVRRVARREDADLFEKAKQLANSEDVLKALKVASVVDKADGGLGIYTGLK
                     NAYDAAKNKDRKRTFEADPQQAADAALKAVALSYMVAALYPGSVADKVRLFLETPAGQ
                     EIAAYYAGVEIALPFTDNLIEASGNWMGNLLESAGGAESRFGEFAGSGPMDQARAILA
                     SLREHIDSTLTRVKPYLDPLREKVSTIAPTALNIADSTTGAVATALDVLPAWRFLGAR
                     LAAEACVVRAMKGI"
     gene            4654..>5351
                     /locus_tag="PLG78_10910"
     CDS             4654..>5351
                     /locus_tag="PLG78_10910"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNE23672.1"
                     /translation="MRGSRHERHLKRLFGAGLRREHFPFLLSGTPHSVDWFEAISENY
                     MDSFGRPRQVLRRMREKFPIALHGVSMSIGQTDARREEYLRKLRILADEIDPFLVSDH
                     LCFTGAHGNLHDLLPLPYTEEAALVVSRNLKEAQDSLGRQIAIENVSCYLRYKSSEMT
                     EWEFLVQCASRAGARILLDVNNVYVNARNHGFDGREFINSIPVDLVAQMHLAGHTDTG
                     DFLFDTHSDHVSEDV"
BASE COUNT         1289 a         1435 c         1308 g         1319 t
ORIGIN      
        1 gcctgtgcac gtgaatgaca gaatgttccg cgcgcaaaga gaagaatgca cggcctactc
       61 ccagccttgg agagaacatg gtggccgcat atctctggac gcgaagaaat gaatcccctt
      121 ttccgtcgga cctgatccag aggttgtcga gcagactcga agcaggctcc gctctgaatg
      181 caattgtgtt tccgaccggc ttcgcatggc cgcgctgcca ggattgaccg tacagggtgc
      241 gaagctcaag accgctctct cgcaagaagc gaatctctcc ccccaactct ggacctttgc
      301 cattcacgtt cgcacgcatg gcagtcagca aaatgttacg aactccgggc tgatagttca
      361 acgtagtgga aacaagtcga gtctggatgt tcgtagagga atagccggcg tacagatgag
      421 gacgaatgaa ccacgtagca ggcaccggag aaaaaccagc gcgcgaaaag attccggttc
      481 gcgttgaatt cgcagcgccg cggccgtcta tcaggcgaag ctgcggattc gggctctgca
      541 agaatgtata accaccgcct aactcatcgt atttggaatg gccgtggatt tgcattgcgt
      601 ctaccaggat aaaccacttc ctggattgat aaccaagaga tccgcgaagt ccaccatacc
      661 cgcgagtgcg aaagtttgcg gggtaactgg gcaaattgga tacacccagc ggattttcct
      721 tttccctctt agtatagtca atggttccgg gagaatagcg attggtggaa aggcccacgc
      781 cgagaaccgg tccggttcgc tgcaaaccag gcggctcatc cggtttgttc tttgattctt
      841 ctctcaatgc cgcctgacgt ttttcctcgg ccagccttcg ttcctcagcg ttctttgcct
      901 cctgtctgcg ttgtccttct tcgcgcgcct tctgctctgc gtcaaacttc gcatcgtcgt
      961 acatgattct cttgatcgtc gccttttgaa gcgaaagtcg gcccgttgca gtatcaatcg
     1021 tgacagacgt acggtcctgg gctatgatgt tcccgcggac atacgtgccg ttctgcatga
     1081 gtatggtttc agaaaataga gctgtagcgg gaaaaagtaa aatcagaaac gaaagacgca
     1141 tggcgtctta ttctaagaac aaacgttcgc acgcaaccac aacacgcacg ttgctatgca
     1201 ttctacaaga aaagaaaatt ctagcgtcag cctgcggaac ttcgctggag actcgtgtac
     1261 gccatagaag gttcacgatt ttctgcatag tctgtagaaa aatgcaagcc cctgctttcg
     1321 cgtctcgcct tcgcagatcg tacaatgagc ttacccacca gagcaagatt tcgcagctcc
     1381 agaatcttat tctggattac agtgcggctg tagaagtcat tgatttcatt ggagagcatc
     1441 tcgattctgc gaagggcgcg atccagtctg agattagatc tcacaattcc aacgtagtcc
     1501 cacatgacgc ttttgatttc acccaggttg tgctggacta aaatccattc ttccgcattc
     1561 gccaggcctt ctttttgcca gcggcgaatc tcaggcctct gtccggatat ttgattttct
     1621 ttaatataac gtccaatgcg atccgcaaac accagcccct ctagaagact gttggatgca
     1681 aggcgatttc caccgtgcac tcccgtacat gagacttcac ccactgcgaa caaatgttct
     1741 atggctgttc ttccattcac atccgttcga actcctccgc acatatagtg agccgctgga
     1801 accaccggga tgcgatcgcg cgaaatctca atccccaggt ccagacaatg agcatagatg
     1861 tttgggaact tcttcatgat ttgctcgcga ggcaaatgag tcacatccag gtgcatgaaa
     1921 gccgcacctg ttctcttcat ctccgcatcg atggcccggg ctacaatatc cctgggtgca
     1981 agatcccctc tttcgtcata tttctttgtg aaaggctctc cgtccggtcc acgcagaatt
     2041 ccgccttctc cgcgcatcgc ttcgctgatg agaaaagtcc tgtgaccggg cgaataaagt
     2101 gcagtcggat gaaactgata gaactccata ttcgagatca cagcgcctgc cctgtacgcg
     2161 agagccacgc catctcctgt ggccacgtca ggattcgtgt tatgcagata aacctggccg
     2221 gcaccgcctg ttgccagaat cgtggcccgg gcgcgaatga tatatatttc ccaggttttt
     2281 ctgtcataca cgtacgcgcc aaagcattga ttggggccag gcggtgcaac acctggaaga
     2341 tacggatctt tttctaaatg atagcaggtg atcaagtcca cagcgctctg gtattctttg
     2401 atctctatgt tccgcgctcg cacagcacgt aaaagcacat cttctacttc tttgccggtg
     2461 agatccttcg catggacaat tcgattttca ctgtgacctc cttcgcgtcc aagggtgaga
     2521 cttccactcg catcctggtc aaaggcagcg cccagttcca taagtctacg aatgtgggtc
     2581 ggaccttccg taacaagaac gcggaccgct tccgtatcgc acagtcctgc acccgcgtcc
     2641 agagtatcgc gaatgtggct ttcaaacgta tcagaagctg caagcacgct tgcgatgccg
     2701 ccctgcgcgt aagtggtgtt tgcgtctgtt acttcttcct ttgtgatgat ggtgactttt
     2761 ccaaattcgc tgagtatcaa cgcagagtag agtccagaga ttccacttcc aatgaccagg
     2821 tagtctgtat caacgatgcg cgtcattttt ttccatctct gcgatcttcg ctctcacttc
     2881 agacatattc aaatgcttgc ccggacaggc agtggcccat agctctctgt gccccacaat
     2941 gtgatcgaga ggaattccat gctctctctt caatcgtaag atcaaatgaa gcaatgcttc
     3001 catctgctga tctggaacgg aatgcttttc aaaatttcca acgagcacaa tggcaatgcc
     3061 aaattgattc acttcgtgca cttttgtaga gtaaccgcca tctctatcat gccatagatt
     3121 gctttcttct acctgccccg ccgctgtccc cgcagtgcca ttattgatta agaagtgata
     3181 cgcaatgtcc ggccaacctt tctctctctg gtgaaggtcg cgaaagtaag caagatttcc
     3241 aaagtcagac gctgtgtgat gtactacaat ccagttccat ctcttctcgc gccagaggta
     3301 acggccacct tcatataaaa gtgctcccgc aatcaaaacg gctgcagcca caatcgccag
     3361 ccgcaatatt cgaggagagc gcatgcgctt ttgtgcggcg cgctttgctg ttgtccatat
     3421 ttttcgcttg ttttacggtc gaggcggggt ttatcctggc gatatggcac tcacacaacg
     3481 cggaaaaggc tgggaatggt tccagtcctg gtacatgctc ctttctcttc catttggttt
     3541 cacaacattc ctggcattct tatacacggg cttccgcgtt aagaaatggc tctggatggg
     3601 tttcggagtg gcttatctac tgctgaacat ctacgtggtt aagaagatcc cggctgcgga
     3661 ttcaggcgtg ccggcagatg accttaccat gggtcttctg ttcggcgagt ggctggttgg
     3721 aatgatccat gtcatcgtca tccgcaaatc gtacctgctc ctcctcgaag caacagaaca
     3781 gagctcaaag atctctgacg acaggcttcg cacaaagatc caggcggact acggagtaag
     3841 cgaaaacaaa attgatgcgg ctctcgttga attcaaagaa acagacgcaa ctgtgcgagt
     3901 cgtaagcatg cttttctctg tatttccatt cattccacaa tatgaatatt atttcagcct
     3961 gaagggtgcc gtgcgaagag tcgcacgcag agaagatgca gatctttttg agaaagcaaa
     4021 acaacttgcg aattcagaag atgtgctcaa agcactgaag gtagcatctg tagtagacaa
     4081 agcggacggc ggccttggaa tttacaccgg actcaaaaac gcctacgacg cagccaagaa
     4141 caaagatcgc aaaagaacgt tcgaagcgga ccctcagcaa gcagcagacg ccgctctcaa
     4201 ggcagtcgct ctttcgtata tggttgccgc tctctatcct ggaagcgtgg cagacaaagt
     4261 gcgcctgttc ctcgaaactc ctgcaggaca ggagattgca gcctactatg caggagtgga
     4321 aattgcactg cctttcacgg acaacctcat tgaagcatcc ggaaactgga tgggcaatct
     4381 tcttgagtct gcgggtggag cagaaagcag atttggagaa ttcgcaggaa gcggacccat
     4441 ggaccaggcg cgagccatcc ttgcctctct tcgcgaacac atcgattcca ctctgaccag
     4501 agtgaagcct tacctggatc ctctacgcga aaaggtgtct accatcgcgc caacagcgct
     4561 caacattgcc gattcaacca cgggtgcagt cgccaccgct cttgatgtat tgccggcgtg
     4621 gagattcctg ggtgcgagac ttgcagcaga agcgtgcgtg gttcgcgcca tgaaaggcat
     4681 ctgaaaaggt tattcggagc gggacttcgc cgcgagcatt tcccgttcct gctttccggc
     4741 acacctcatt ctgtagactg gtttgaggcg atttcagaaa actacatgga ctccttcggg
     4801 agacccaggc aagtacttcg ccgcatgcgc gaaaagtttc ccattgcact tcatggcgta
     4861 tccatgtcga tcgggcaaac cgacgcacgc cgggaagaat atttaagaaa attgagaatc
     4921 ctcgcagatg aaatcgatcc atttctggtt tcagatcatc tgtgcttcac cggagctcac
     4981 ggaaacctgc acgaccttct gcctctgccg tatacagaag aagcagccct ggtggtatcg
     5041 cgaaatctca aagaagcgca ggatagcctg ggacgccaga tcgccattga aaatgtttcg
     5101 tgttacttaa gatacaaatc atccgaaatg actgagtggg aattcctggt ccagtgcgct
     5161 tctcgcgcag gcgcgcgcat ccttctggat gtaaacaatg tgtacgtaaa cgcgcgcaac
     5221 cacggctttg atggcaggga gttcataaat tccattccgg tggatcttgt agcacagatg
     5281 catctagccg gccacacgga tacaggagat ttcctgtttg atactcactc ggatcatgta
     5341 agcgaagatg t
//
LOCUS       DAOQJL010000233         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8913_length_5345_cov_4.127599, whole genome shotgun sequence.
ACCESSION   DAOQJL010000233 DAOQJL010000000
VERSION     DAOQJL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8913_length_5345_cov_4.127599"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLG78_10915"
     CDS             <1..1318
                     /locus_tag="PLG78_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE23673.1"
                     /translation="TGKWSLPVNAGPKINSEADEISPSFSPDRGMLFFSSNREGGLGH
                     FDIYVSRFREDSFEAAENLGRPFNSERDDEYFTITEDRLWAYFASERQHVDAAGRLDL
                     YRTALPDWLRDPVDVAFSMLIMDASTRLPLGLEATVKIQWEKDSIVRHSKPFNKDPKD
                     LEKSNFEQSLVTGRQYRVVISAPGFYPQEILLDYRGSVPSSKKDHRIVYLEPVKQTNP
                     EESRIVRGRIVDAATDLPLSSTRLRKVTAGKDAEKIETIPTDNKGHFELRLTKGEKFT
                     LRADAPGYTQTTESFEEKETLKEIVLRLKPSSGDPCKGDLPECIENLRIFFALGASDL
                     SPSEKRKLDAVIRILKKNPDLRIEIRGHTDRTFRGPEQNSMAYNQKLSEIRSASVRRA
                     LVEGGIDTGRLITVGNSYLEPEESETSAAGRDRNRRVEFRRLSLDK"
     gene            1368..2498
                     /locus_tag="PLG78_10920"
     CDS             1368..2498
                     /locus_tag="PLG78_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNE23674.1"
                     /translation="MEILRDKKRDWDLLREADKIQYILRDKLRSKPFYLKGSEPPVEF
                     IMGNILPEGIEFFTIPEQSPQTQVTLYLTLNRHIEVDFEVKNIQDDRWLLMPTLARIG
                     KVVRAHPRFAMAEGSVYATNFKVSKNELGADIARLQIAAQVVLRDQEKQLAEEFPGLR
                     MFLTADRDRPLGLRPIEHMTETLVIKDTNDPHAYEIPVGDTTYFKLLGKTFEQVRLKL
                     GDTASLVIYPIALEEKGKKYLIAHMYLPLKHTGTDFDTLLKTLSEKAELIIERIREVN
                     MVTIKEKQKVRDISLGGVGIEIDNSDLKKYLPVSDRITFDLIFKMQAPLRFQGKICHV
                     AAVGDETLLAGIDIEGTGHTEYRKSNQERLGTLIQLIRSESK"
     gene            2498..3778
                     /gene="hisS"
                     /locus_tag="PLG78_10925"
     CDS             2498..3778
                     /gene="hisS"
                     /locus_tag="PLG78_10925"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNE23675.1"
                     /translation="MAGPYVGTRDFYPEDMQFRNAMFAVIRSVCESYGYLEYSAPLLE
                     PISLYQSKSSEEIVNDQVYRFTDRGDREVAIRPEMTPTMARMVSARGMELIRPVRWYS
                     IANFMRYERPGKGRLREFYQLNADLLGPATPAADAEILSLLIDILTAFGAKQGDFLLR
                     VSDRRLFDSYVQHTDKEVRRSIGRLLDKREKIGEEAFQEELRRTADGPLVDRVNSFLS
                     IRLEDLEKLGQKGEIDAAVGGHLSAVSDILSAQGKSAFLSFDPGIVRGFDYYTGLIFE
                     AQDLNPENRRAICGGGRFDRLLGQMGKNELPAVGFGLGDVTLEQFLRSRGFLADASPK
                     KGCFFILFPESRDASLKIASDLRKAGVLVETSLEPSVKIGKQMEAADKKGRRFVLMMG
                     GDELARGVVRVKDLATGDQTDVKPSDLSSHLNRA"
     gene            3794..4468
                     /locus_tag="PLG78_10930"
     CDS             3794..4468
                     /locus_tag="PLG78_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNE23676.1"
                     /translation="MRFRIVIFTFCFVLSCARVAAVPPSTRVVFFGDSITEIGVRQGG
                     YIQRMQTILSERGLSDRFELMGAGVSGDKIYDLYLRLEDDVFARNPQVVVVWVGVNDV
                     WHKQIHNTGTDAVKFKRFYVALIRKLQARNMRVILVTPMGVGEKRNLANPLDQELDQY
                     SALIRDLAVQYHCDLVDMRAAFLKYVGSHNPLDRESGILTYDRVHPNDTGNQFVANEM
                     LRVLLR"
     gene            complement(4645..>5345)
                     /locus_tag="PLG78_10935"
     CDS             complement(4645..>5345)
                     /locus_tag="PLG78_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNE23677.1"
                     /translation="IMGFEIDPGFQRMMIRMSLAALCGLAIGMERGYRGKAAGVRTNL
                     LICLGAALFMIVSEEVAYEAKSNGFPGPDPARIAAQVVTGIGFLGAGAILTNRGTVTG
                     LTTAASIWCVSAIGLCAGAGLYRLAMAATLGILFTLEFFTFFERRIRTTRFRYMRLEV
                     TIKKEARVQEVRRVLRNLKIQFSNEETRNILGETHYIATLYYKGELEKMIEDELMLVS
                     GVRDVNFLGEGMDT"
BASE COUNT         1399 a         1261 c         1406 g         1279 t
ORIGIN      
        1 aacaggcaag tggtctttgc ccgtgaacgc cggaccgaag atcaactctg aggcggatga
       61 gatttcacca tccttttctc cagatcgcgg gatgcttttc ttcagttcga atcgagaagg
      121 tggtctgggg cattttgata tttacgtaag tcgtttcaga gaagattcct ttgaagcggc
      181 ggaaaatctg ggtcgtcctt ttaattctga aagagacgat gaatacttca ccataacaga
      241 agacagactc tgggcctatt tcgcaagcga gcgccagcac gtagatgcag ccggcagact
      301 ggatttgtat cgcactgctc tgccggactg gcttcgcgat ccagtggatg ttgcattctc
      361 catgctcatc atggatgcat ctaccagatt gcctcttggt ctggaagcaa ccgtgaagat
      421 tcaatgggag aaagactcca tagtccgtca tagcaagcca ttcaacaaag atccaaagga
      481 tttagaaaag agtaattttg aacagtctct cgttactgga aggcagtata gagttgtaat
      541 ctccgcacct gggttttacc cacaggagat tcttctagac tatcgcggaa gtgttccatc
      601 cagcaaaaag gatcacagaa tcgtttatct ggaacctgtg aagcaaacca atccagaaga
      661 atccagaatt gtccgcgggc gaattgtgga tgctgctaca gatctacctc tatcttctac
      721 gcggcttcgc aaagtaacgg caggaaaaga cgcagaaaaa atagaaacga ttccaacaga
      781 caacaaagga cattttgagc tgcgtcttac gaagggggag aagttcactt tgcgagcgga
      841 tgctccgggc tacacacaaa caactgaatc gtttgaggaa aaagagactc tcaaagaaat
      901 tgtactgcgt ctcaaaccat cttctggaga tccatgcaag ggagacctgc cggaatgcat
      961 tgagaatctt cgaatattct ttgcacttgg tgcatccgat ctttccccga gtgaaaagag
     1021 aaagctcgat gcggtgattc gcattttgaa aaagaatcca gatcttagaa tagagattcg
     1081 cggacacacg gaccgcactt tccgcggacc cgaacagaat tcaatggcat acaatcaaaa
     1141 gttatcggag attcgttccg catctgttcg tcgcgctctt gtagagggtg gaattgacac
     1201 cgggcgtctg ataacagtgg gcaacagcta tctcgagcca gaagagtcag aaacatcagc
     1261 tgccggacgt gacagaaaca ggcgcgtcga gttcaggaga ttaagtcttg ataaataact
     1321 ctgtgcaagc actatcgccg caggccacgg attcgtctaa ttgagctatg gagatacttc
     1381 gcgacaaaaa acgtgactgg gatttgctgc gggaagcaga taaaatccag tatatcctcc
     1441 gagacaagct gagaagcaaa cccttctatt tgaaaggatc tgagccaccc gtcgaattca
     1501 tcatgggcaa catcttgcct gaaggaattg agttttttac cattcccgag caatcgcctc
     1561 agacccaggt cactctgtat cttactctca atcgccacat tgaagtggac ttcgaagtaa
     1621 aaaacattca ggacgatcgc tggcttctca tgccaacact tgctcgtatt ggcaaagtgg
     1681 tgcgcgcgca tccgcgtttc gccatggcag aaggaagtgt atacgctacg aatttcaaag
     1741 taagcaagaa tgagctggga gctgacattg caagactcca gatcgcagcg caggttgtgc
     1801 tacgcgatca ggagaagcag cttgcggagg aattcccagg cctacgtatg tttctgactg
     1861 cggacagaga tcggccattg gggcttcgcc ccattgagca catgacggaa actctggtca
     1921 tcaaggatac caatgatcct cacgcatacg aaatcccggt aggggacaca acgtacttca
     1981 agctactcgg aaagacgttc gaacaggttc gtctcaagct aggagacacc gcgtcccttg
     2041 taatctatcc aattgccctg gaagaaaagg gaaagaaata tctcatagca cacatgtatc
     2101 tccctctgaa gcatactgga accgattttg atacgctact caagaccctc agcgaaaaag
     2161 ctgaattgat cattgaacgc attcgcgaag tgaacatggt caccatcaag gaaaagcaaa
     2221 aagtccgtga tatctcgctg ggaggagtgg gcattgagat cgacaacagc gatttgaaga
     2281 aatacctccc ggtcagtgat cgcataacgt ttgatttgat ttttaagatg caggcgccgc
     2341 tcagattcca gggaaagatt tgccacgtgg cagcagtggg ggatgagaca ttgcttgccg
     2401 gaattgacat tgagggaacg gggcacacag aatacaggaa gtcgaatcag gaacgactgg
     2461 gcacattgat ccagctgatt cgttcggaat cgaagtaatg gctgggccct atgtgggaac
     2521 gcgggatttc tatcccgagg acatgcaatt tagaaatgca atgtttgctg tgattcgttc
     2581 tgtgtgcgaa tcgtacggct atctggaata ttcggctcct cttcttgagc caatcagcct
     2641 ctaccagtct aaatcatcgg aagaaatcgt aaacgaccag gtgtatcgct ttacggatcg
     2701 cggagacaga gaagtcgcca tccgtcctga gatgactcct acgatggctc gcatggtttc
     2761 cgcacgcggc atggaattga ttcgtcctgt gcgctggtac agcatcgcga acttcatgcg
     2821 atacgaaaga cctggcaagg gcaggctgcg cgaattttat cagttgaatg cggacttgct
     2881 tggtccggcc acgcccgcgg cggacgctga aatcctgagt ctgttaattg acatattgac
     2941 tgcgtttggt gcgaagcagg gagactttct tcttcgcgta agcgatcgta gattgtttga
     3001 ttcttatgtg caacacacgg acaaagaagt caggcgatcg attggcaggt tgctggataa
     3061 gagagaaaaa attggagaag aagcgttcca ggaagaattg cgtcgcacgg cggacggtcc
     3121 gctcgtggat cgcgtaaatt cttttctgtc catccggctt gaagatctgg agaaactggg
     3181 ccagaaggga gaaattgatg cagccgtggg cggccatctc tctgcagtca gtgatatact
     3241 atcggcgcag ggtaagtctg ctttcctttc ctttgatcct ggaattgttc gcgggtttga
     3301 ttattacact ggtttgattt ttgaagcaca ggatttgaac ccggaaaatc gaagggccat
     3361 ctgcggcggc ggcaggtttg acaggttgct cggacagatg ggaaaaaacg aactgccggc
     3421 tgttggtttt ggtctggggg acgtaacact cgaacagttc cttcgcagcc gtggctttct
     3481 ggcagatgcg tcacccaaga aaggttgttt ttttatacta ttccctgaaa gcagagacgc
     3541 ttcccttaaa atcgcatctg acttgagaaa ggcgggcgtg ctggtagaaa catctcttga
     3601 accatctgta aagattggaa agcaaatgga ggcggcggat aagaaaggcc gcagatttgt
     3661 gctcatgatg ggtggagatg aactggccag aggagttgtg agagtaaaag atcttgcaac
     3721 aggggaccag acggatgtca aaccatcgga tctgtcctct cacctgaatc gcgcttagta
     3781 ttgagcgatc gctatgcgat ttcgaatcgt aatcttcact ttctgcttcg tcctttcctg
     3841 cgcgcgtgtg gctgcggtcc cgccatctac cagagttgtt tttttcggcg attcgatcac
     3901 tgaaatcggc gtgaggcaag gtggatacat ccagcgcatg cagaccattc tatctgagag
     3961 aggactttcg gatcgctttg agcttatggg agcaggagtg agcggagata aaatctatga
     4021 tctctacttg cgtctggaag atgatgtatt tgcgcgcaat ccccaggtag ttgtggtttg
     4081 ggtgggagtc aatgacgtct ggcacaagca aatacacaat acaggcacag acgcagtcaa
     4141 gtttaagcga ttctatgtcg cgctcattcg taaattgcag gcgcgaaata tgagagtgat
     4201 tctggtgaca ccgatgggtg tgggggaaaa aagaaatctg gcaaatccgc tcgaccagga
     4261 actcgatcag tactctgccc tcattcgcga ccttgcagtt caatatcact gcgatcttgt
     4321 ggatatgaga gctgcttttt tgaagtatgt gggctctcac aatcctctgg accgcgaatc
     4381 cggaattcta acgtacgaca gagttcatcc aaatgacacg ggcaaccagt tcgtcgcaaa
     4441 cgaaatgctg cgagtacttt tacgttgata tgcggcacga aaggctcaga accttgccga
     4501 cacttagcct ctcagcgtac gaaccggcat gtcttgccca attgcggagg caggatgcca
     4561 gagccccggc gcgagtcgga catggacgac cgcgtctcgc gccggttggg caagggcatg
     4621 tcgtttcgca aaggccagag aggactaagt gtccattcct tctccgagaa aattcacatc
     4681 gcgcacaccg gacacaagca tcagttcatc ctcgatcatt ttttccagtt cgcctttgta
     4741 atacaatgtt gctatatagt gagtttcgcc gagtatgttg cgcgtttctt cgtttgagaa
     4801 ttggattttt agatttctta aaacgcgacg cacttcctgc acacgggctt ctttcttaat
     4861 ggttacttcg aggcgcatgt agcgaaagcg tgtggtgcga attctgcgct caaagaacgt
     4921 gaaaaactcc agcgtaaaca ggatgccaag agtggcagcc atggcgagtc tgtagagccc
     4981 ggcgccggcg cacagtccga tcgcagaaac acaccagata ctggcggccg ttgtgagacc
     5041 tgtgacagtt ccgcggttgg tcaggatcgc tcctgcaccc agaaagccaa tgccagtgac
     5101 cacctgggct gcgatgcgtg cagggtctgg tcctggaaat ccattggact tcgcttcgta
     5161 ggccacctct tctgaaacga tcatgaagag tgccgcgccc aggcagatga gcagattcgt
     5221 tcgcactccc gccgcttttc cgcggtaacc gcgctccatt ccaatcgcca gcccgcacag
     5281 cgcggcaagc gacatgcgaa tcatcattct ctgaaaccca ggatctattt cgaagcccat
     5341 gatta
//
LOCUS       DAOQJL010000234         5326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8959_length_5326_cov_4.414343, whole genome shotgun sequence.
ACCESSION   DAOQJL010000234 DAOQJL010000000
VERSION     DAOQJL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8959_length_5326_cov_4.414343"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLG78_10940"
     CDS             complement(<1..896)
                     /locus_tag="PLG78_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNE23678.1"
                     /translation="MEKNAKIYIAGHRGLVGSAIVRVLAEQGYTNVMGRTHSELDLTR
                     QPDVDAFFSKEKPDYAILAAAKVGGIHANNTYPAEFIYSNLQIQNNIIDACYRHGVKK
                     LLFLGSSCIYPKFAHQPMSESELLNGKLEPTNEPYAIAKIAGIVMCQSYNRQYGTNFI
                     SAMPTNLYGPGDNYHPENSHVIPALIRRFHEAKKNNVKEVVIWGTGSPLREFLFSDDC
                     ARACLFLMERYDVRGDSTGGEIVNIGSGSEISIRDLAKTVGEVVGFRGDIIFDVTKPD
                     GTPRKLLDVAKLHKMGWRHTVE"
     gene            complement(902..1945)
                     /gene="fba"
                     /locus_tag="PLG78_10945"
     CDS             complement(902..1945)
                     /gene="fba"
                     /locus_tag="PLG78_10945"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775027.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNE23679.1"
                     /translation="MALVPMRLLLDHAAENNYGIPAFNVNNLEQVLAIMRAADRADSP
                     VILQVSRGARAYAGEHFIRHLILAAVESYPHIPISLHQDHGNSPATCFSAIRNGFTSV
                     MMDGSLKEDGKTPADFEYNARVTADVVRVAHSIGVSVEGELGCLGSLETMTGDKEDGH
                     GAEGKLTREQLLTDPDEAVAFVQKTNVDALAVAIGTSHGAYKFKQKPTGEVLVIKRIE
                     EIHKKLPNTHIVMHGSSSVPQDYLAIINKTGGKIAETYGVPVEEIQKGIKVGVRKINI
                     DTDLRLGWIAAVREAAAKKPEEFDPRYFLKPAMKNMEDVCADRFAKFWCAGNGSKIKQ
                     VGLDEYAKKYSAG"
     gene            complement(1947..2480)
                     /locus_tag="PLG78_10950"
     CDS             complement(1947..2480)
                     /locus_tag="PLG78_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE23680.1"
                     /translation="MRAILLDRDDTINEDPGYLADATQVRLLPRVVEGLSLLRSHGYR
                     FFVMSNQSGVARGLISGPELQAVNDRISELLLAQGIVIERFYVCPHRDEDGCDCRKPK
                     PGLFRQFFQDWNASGSQCFAIGDKPRDLEAARPFGVRGILLQAGKEAPVYFPKNLVYR
                     AVDLLDAADYIIRTEGD"
     gene            complement(2477..2989)
                     /locus_tag="PLG78_10955"
     CDS             complement(2477..2989)
                     /locus_tag="PLG78_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNE23681.1"
                     /translation="MHDIERLSEGEARLIEALAERSSSQRILARAAGLSLGMTNILIK
                     RLVKKGLIKVVTLNGRTLSYMLTPRGFAEKVKRTYEYITVSIRFIDRVRSNILSIVAA
                     LPEGSPLYIAGPGELAQLAAETLRESGRVFEQVNTVADLEGKTGVCLVCLPEMPVPIP
                     PGLILKSIVA"
     gene            complement(3002..4063)
                     /locus_tag="PLG78_10960"
     CDS             complement(3002..4063)
                     /locus_tag="PLG78_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004782145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNE23682.1"
                     /translation="MYAPPLKMKMLVTGSAGFIGFHLVERLLSEGHQVVGIDNLNPYY
                     DPALKVSRLRAAGIEDPVPGSIVHARQRSYGFLQGDITNSTDLKSVFDLGPFDCVCHL
                     AAQAGVRFSLEQPLAYVHANIVGTTHLLEACRSDAPHFLFASSSSVYGLNQKMPYSVH
                     DNTDHPVSIYAASKKASELIVHSYSHLFGIPSTGLRFFTAYGPWGRPDMALFKFTQSI
                     LKGEPIQVYNNGRMQRDFTFIDDIVTGVVKLAHHIPQPDEGWDANHPDPSTSSAPYRI
                     HNIGNSRPTELLDYVRAIERALGKQAVLEMLPAQPGDVTSTWADTSELQKEIDFIPDT
                     AIDVGVSKFVAWFRQYYNV"
     gene            complement(4159..5094)
                     /locus_tag="PLG78_10965"
     CDS             complement(4159..5094)
                     /locus_tag="PLG78_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE23683.1"
                     /translation="MLPRIGVTSVSFSKNQTLRAELLSVFPESCFNDSGKMSEADVIS
                     FLARCDAAIIGTEPVTGRVIESLPGIGMYAKYGVGLDNIDLEVLATRGIPLGWTGGVN
                     RRSVSELALWFMLSLCRNTVHTSRQLASGIWDKDGGRLLSGLTVGIVGCGFVGEDLLS
                     LLSAFGGQRLIVDIEDRSGVALRFGARQVSYSEALSSADILSFHVPLTEATRGMLGSK
                     EIARLTPGAIVINTSRGSVFDEAALKEALRSGRIQAGLDVYSTEPPTDLELLAQKGLV
                     CTPHVGGNAREAVLAMGRSAISHLVHHFGQKKPSD"
     gene            complement(5118..5191)
                     /locus_tag="PLG78_10970"
     tRNA            complement(5118..5191)
                     /locus_tag="PLG78_10970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5155..5157),aa:Val,seq:gac)
BASE COUNT         1315 a         1440 c         1318 g         1253 t
ORIGIN      
        1 aattctactg tatggcgcca gcccatcttg tgcagcttcg caacatctaa caatttcctg
       61 ggagtcccgt ccggctttgt tacatcgaat atgatgtcac cgcgaaagcc aacgacctcc
      121 cccacagtct ttgcgagatc ccgtatggaa atttctgacc cggatccaat gttcacaatt
      181 tcgccgcccg ttgaatctcc gcgaacatca tatcgctcca tcaagaagag acatgcacgc
      241 gcgcaatcgt ctgaaaaaag aaactcgcgg agaggcgagc ctgtccccca gatgactacc
      301 tctttgacat tgttcttctt tgcctcatgg aacctgcgaa tcagcgccgg gatgacatgc
      361 gaattctccg gatgatagtt atctccaggt ccgtaaagat tcgtaggcat agcagagatg
      421 aagttcgtcc cgtattgacg gttgtagctc tgacacatga ctattcccgc aattttcgca
      481 atcgcatagg gctcatttgt tggctcaagc tttccattca gcaactcaga ttcgctcatg
      541 ggttgatgag cgaactttgg atagatgcac gaagagccaa ggaataagag cttttttacg
      601 ccgtgacggt aacatgcatc tatgatgttg ttctgtattt gaaggtttga ataaataaac
      661 tctgcgggat acgtattatt cgcatgaatc cctccgacct tcgccgcggc cagaatagca
      721 tagtccggct tttcttttga aaaaaaagca tccacgtcag gttggcgagt cagatccaat
      781 tcggaatggg tacgacccat cacgttcgtg taaccttgct cggcgagaac gcgaacaatc
      841 gcggagccga cgagtccgcg atgacctgca atgtatatct ttgcgttttt ttccattcgt
      901 ttcagcctgc ggaatatttt ttcgcgtatt catccagtcc gacttgcttg atcttcgaac
      961 cattgcccgc gcaccagaat ttagcgaaac gatctgcgca aacatcttcc atgttcttca
     1021 tagcgggttt aagaaagtat cttgggtcaa attcctcagg tttcttggcc gcagcttcgc
     1081 gcacagcagc aatccaaccc aggcgaagat ccgtatcgat attgatcttg cgcactccga
     1141 ccttgatccc cttctgaatt tcttccaccg ggacaccgta ggtttccgcg atctttccac
     1201 ctgtcttgtt tatgatggcg agatagtctt gcggcacact ggaagaacca tgcataacga
     1261 tgtgtgtatt tggaagtttc ttatgaattt cttctatgcg tttgatcaca agaacttcgc
     1321 cggttggttt ctgtttgaac ttgtaagctc cgtgactcgt gccaattgca accgcaagtg
     1381 catccacatt cgtcttttga acaaacgcaa ctgcttcatc aggatccgtc agtagttgct
     1441 cgcgcgtaag tttcccttcc gctccgtgcc catcttcttt gtcgcccgtc atagtttcca
     1501 gagaacccag acatccaagc tcgccttcta cgctcacacc aatggaatgc gcaacgcgaa
     1561 ccacgtccgc ggtcacgcgc gcgttgtatt cgaaatcggc gggagttttg ccgtcttctt
     1621 tcagggaacc atccatcatg acggaagtga atccgttgcg gatggcggag aaacaggtgg
     1681 ccggactgtt accgtgatcc tgatgtagcg aaataggaat atgaggatag gactcgacag
     1741 cagcgagaat caaatgtcga ataaaatgct ctccggcata ggcccgcgcg ccgcgcgaaa
     1801 cttgaaggat gacaggactg tccgcccggt ccgccgctcg catgatggcg agcacctgct
     1861 ccagattatt tacattgaac gcgggaatcc cgtaattgtt ttctgcagcg tgatcgagca
     1921 gcagcctcat cggtacaagc gccatactag tctccttcag tcctgattat gtagtccgcc
     1981 gcatccagaa gatccacggc cctgtagaca agatttttcg ggaaataaac aggcgcctct
     2041 tttccggcct gcaaaagaat cccacgaacg ccaaatgggc gagcagcttc caaatcgcgt
     2101 ggtttatcgc cgattgcgaa acactgagaa cctgacgcgt tccaatcctg gaagaattgg
     2161 cgaaacaggc caggctttgg ctttcgacaa tcgcatccat cttcatcgcg gtgcggacaa
     2221 acatagaagc gttcgattac tattccttga gcgagcaaca attccgagat tcgatcgttg
     2281 accgcctgca gctctggccc actgattaag cctctggcta ccccggattg attgctcatt
     2341 acgaaaaacc gataaccatg agaacgcaac agactcagcc cctccactac gcgcgggagt
     2401 aaccgaacct gagtcgcatc tgcgagatag ccagggtctt cgttgattgt atcgtcccga
     2461 tcaagcagaa tggctctcat gcaacgatgc tcttgagaat cagtcccgga ggaatgggga
     2521 caggcatttc cggaagacac acaagacaaa ctccggtctt accttccaaa tccgcaaccg
     2581 tgttcacctg ctcaaaaacg cgtccggatt cgcgcaaagt ctcagcagcc agctgcgcca
     2641 gctcacccgg acccgcgatg taaagaggcg aaccttccgg cagagcagct acgatcgaaa
     2701 gaatgttact tcgcacacga tcgatgaatc gtatggagac ggtaatgtac tcatacgttc
     2761 tctttacctt ttccgcgaaa ccacgcggcg tgagcatgta acttagagtc cgaccattca
     2821 gagtcaccac tttgatcagt cccttcttta caagacgctt gatgagaatg tttgtcatcc
     2881 cgagggacaa acctgcggcc cgtgccagaa tccgctgact gctgcttctt tcggcgagcg
     2941 cttctatgag ccgcgcttca ccttcgctca atcgttcaat gtcatgcatg atggagagaa
     3001 actaaacgtt gtagtattgc ctgaaccagg cgacaaactt gctgacgcca acgtcaatcg
     3061 ccgtatcggg tatgaagtcg atttccttct gtagttcaga agtgtctgcc caggtagacg
     3121 tcacatcgcc tggttgcgca ggaagcatct caagcactgc ctgtttaccg agagctcgct
     3181 caatggcgcg aacatagtcg agcaattctg tcggcctgga attcccaatg ttatgaatgc
     3241 gatatggcgc cgaagatgta gagggatccg gatgattcgc gtcccagcct tcatctggtt
     3301 gaggaatgtg gtgcgccagc ttcaccactc cggtcacgat atcatctatg aaagtaaaat
     3361 cacgctgcat gcgcccgtta ttgtacactt gaattggctc gcccttcagg atcgactgtg
     3421 tgaacttgaa gagagccata tctggcctgc cccagggccc atacgcggtg aagaatcgaa
     3481 gacctgtgga agggatgccg aacaggtggc tgtacgaatg gactatgagc tccgatgctt
     3541 tcttactggc ggcatagata ctcactggat gatctgtgtt gtcatgaact gagtaaggca
     3601 ttttttgatt cagtccgtaa acggaggaac tggaagcgaa tagaaagtgg ggcgcgtcag
     3661 atcggcaggc ttctagaaga tgggtcgtgc ccacgatgtt ggcatgcaca taggccaggg
     3721 gttgctcaag actaaatcgt acgcctgcct gcgctgccag atggcagacg caatcaaacg
     3781 gcccaagatc aaatacggac ttcaaatcgg tcgaatttgt tatgtccccc tgcaagaagc
     3841 catacgaccg ctgtctggca tggacgatcg accctggcac tggatcctca atacctgcgg
     3901 ccctcaagcg actgactttg agagcgggat cgtagtaagg attcaagttg tctattccaa
     3961 caacttgatg gccttcggag agcagccgct ccacgaggtg gaatccgata aacccggctg
     4021 aaccggtgac tagcattttc atcttaagag ggggcgcgta catggcttcg ccaggtgagt
     4081 cccaggggga aaaaaggcac cttccagggt gagccggaga ggctttgcgt caatcgattt
     4141 tgttcaacga ttgaacgatc aatcagacgg ctttttttgg ccgaaatggt gaaccaggtg
     4201 agaaatggcc gaccgcccca tggcaaggac cgcttcccgg gcgttaccgc ctacgtgcgg
     4261 ggtgcatacc agtcctttct gggcaagtag ctcaaggtcg gttggtggct ccgtcgagta
     4321 aacgtcaagc ccggcctgga tgcgaccgct cctgagcgcc tctttcaagg cggcttcgtc
     4381 aaaaacggag ccacgggatg tgttgatcac aatggcgcct ggcgtgagtc gagcgatctc
     4441 tttggagccc agcatcccac gtgtagcctc ggtcaatgga acatgaaagc ttaagatgtc
     4501 agcggaagaa agagcctctg aataagagac ttgccgtgct ccgaatctaa gggcaacgcc
     4561 cgagcgatct tcaatgtcca caatcaggcg ctgtccacca aacgcggaaa gtaaagaaag
     4621 caaatcttcg cctacaaaac cgcatcccac tatgcccacg gtgagtccgg agagaagtct
     4681 cccgccgtcc ttgtcccaga ttccagaagc aagctgcctg ctcgtatgca ctgtgttgcg
     4741 acagaggctg agcatgaacc agagggcaag ctcgctgacg ctgcgccgat tcactccgcc
     4801 cgtccaaccc agcggaatgc cccgagtggc caggacctcc aaatcaatgt tgtcgagacc
     4861 gacaccgtac ttcgcataca taccaatgcc aggtagagat tcaatgaccc ttcctgtcac
     4921 gggttctgtg ccaatgatgg cagcatcgca ccgggcaaga aaagaaataa catctgcttc
     4981 cgacatcttt ccagagtcgt tgaagcaact ttccggaaac accgaaagca actctgctct
     5041 tagagtctga tttttcgaaa aagaaacgga tgtaacgccg atgcgcggca gcatttagat
     5101 cgccggggct ttcaaactgc gagctactgg aatcgaacca gtgacctctt ccatgtcaaa
     5161 gaagcgctct aaccagctga gctaagcccg cgtcctcttt cagagattct ttcgacgcag
     5221 aggtcgtcaa tgaatattcg aagaccgcca cttccagaat acgaagcctg cgaccgcaac
     5281 cacagcaacg atgataaata ccgtattgta caacttcaaa tataca
//
LOCUS       DAOQJL010000235         5317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_8982_length_5317_cov_4.735272, whole genome shotgun sequence.
ACCESSION   DAOQJL010000235 DAOQJL010000000
VERSION     DAOQJL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8982_length_5317_cov_4.735272"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..2517)
                     /locus_tag="PLG78_10975"
     CDS             complement(49..2517)
                     /locus_tag="PLG78_10975"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNE23684.1"
                     /translation="MKKVSGSKGLGSSIAQRKTTSVLRKYMPLLLIVAFFAPFSIFAS
                     EADLKIPDLSSVNFAIGNGKTLLFVGMGICVLGMLFGLMEFTAIKNLPVHIAMREISE
                     LIYETCKTYLLQQGKLLLVLEGFIGVIMVVYFGFLRHMPATTVLVILLCSLIGIAGSY
                     GVAWFGIRINTFANSRTAFAALRGKPFPNYAIPLRAGMSIGLLLISIELFLMLCILLL
                     IPAEYAGPCFIGFAIGESLGASALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGV
                     IADCTGDNAGDSVGPTADGFETYGVTGVALISFILLAVKDPTVQASLLVWIFVMRIVM
                     ILASSGSYGINALIAKARYGNADDMNYEHPLTVLVWLTSFVSVALTYVVSYVLIPNLG
                     GDTTIWWKLSSIITCGTLAGAIIPELVKVFTSTESGHVREVVSASKEGGASLNILSGL
                     TAGNFSAYWMGITIAALMAGAYGISTMGIEGPLGMMAPAVFAFGLVAFGFLGMGPVTI
                     AVDSYGPVTDNAQSVFELSLIETRKGAREEIKKEFGFDPHFIKGKEFLEKNDGAGNTF
                     KATAKPVLIGTAVVGATTMIFSIIVILTKGLQPGLLANLSVLHPPFLLGLIVGGAVIY
                     WFSGASTQAVTTGAHRAVEFIKENIKLEGVEKASVEDSKKVVEICTIYAQKGMFNIFL
                     VVFFSTLAFSFVGEYFFIGYLISIAVFGLYQAIFMANAGGAWDNAKKLVETELKAKGT
                     PLHDASVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVELAIEIPADMLGVRLGLAG
                     VFFLISAVFVWRSFYAMRIEHIGD"
     gene            2626..3939
                     /locus_tag="PLG78_10980"
     CDS             2626..3939
                     /locus_tag="PLG78_10980"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNE23685.1"
                     /translation="MAEGGISLSNAWILPFVIFLLCVAIIPLINHHFWDRHLWWFSLG
                     FFCFPMVYALVFILGGPFIQKTVEKCMEYVSFMILLASLYVISGGILIRGHFRGTPLV
                     NTCFLVVGSVFASLIGTTGASMLLIRPLLRANAGRKRTAHIVVFFIFTVSNIGGLLTP
                     LGDPPLFLGYLQGVPFLWTIQLWKEWLFVLTLVIALFWLVDAYILRKDGKGIDLHHHD
                     TTEKFHISGVLNLILLLGVVASIFFHGYFLRTVSGWPHFGPQEGSMLVLTCVSLLAMP
                     VSHEIRLQNGFSWKPILEVMFLFAAIFATMIPALHILETRATSLGISAPWQFFLASGL
                     LSSLLDNAPTYLAFLSVAKGLALANDAGFVFHDGTHVSAAILAAISCGSVFMGANTYI
                     GNGPNFMVRAIAAERGVKMPGFFGYMGYSAAILLPIFALVAWIFF"
     gene            complement(3960..4961)
                     /locus_tag="PLG78_10985"
     CDS             complement(3960..4961)
                     /locus_tag="PLG78_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23686.1"
                     /translation="MRLLAICFFLTTTALAARDPEGLRLRASIFGGGYTPDLQEKYAN
                     DRLYGSFVAGGIGANAPWNNKNASGLPLEAEYSTHPFHFELEIASIHSRPDYAGVSAV
                     SLTSSTIRYDVQQYDTTNVELSRFTPVLLARYDLTSQDSAHEISILGGIRRVDTRATY
                     DYKYAQRFTISQSGLSVSGSVYGQSFDFRSRGVGTGLIFGGDYRYHLENGSNFKLRLA
                     LFSADGSWFQNRETADVNTGGSGSGLIRREDGGYHVNGVEFIAGYFHSVSENIRIFAQ
                     LRTETSAIRDNKVRLTRISQPTATEFQNLLIDSALTYPGSHAKDTMGGLQAGVEVIL"
     gene            5036..>5317
                     /locus_tag="PLG78_10990"
     CDS             5036..>5317
                     /locus_tag="PLG78_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23687.1"
                     /translation="MTPEAITGFENRLIRMHKHIGKWARRQGITCFRIYDRDVPQFPL
                     CIDKYEDFIIVSEYRTSKTAEGDEHDQWIDLCRQIIAKTLSVPIAHIDFR"
BASE COUNT         1388 a         1325 c         1303 g         1301 t
ORIGIN      
        1 aaggcgcccc ttgcgaggcg cctttcatca atcgagaatc agagacgatt agtcgccgat
       61 gtgctcaatt cgcatagcgt agaaggatct ccatacaaac accgcagaga tcaggaagaa
      121 cacgcctgca agtccaagac gcacgccaag catatctgct ggaatctcga tggcgagctc
      181 cacagccaga aggccaaaca gggttgtgaa cttgatgatt gggttcatgg ccacagacga
      241 tgtatctttg aacggatcac ctacagtgtc tcccacaacg gaggcatcgt gaagcggagt
      301 tcccttggct ttcagttcag tttcaacaag cttcttcgca ttgtcccagg caccgccggc
      361 attcgccatg aagatcgcct gatacagacc aaacacagca atggagatga ggtatccaat
      421 gaagaagtat tctccaacga atgagaaagc aagtgtgctg aagaacacaa cgaggaagat
      481 gttgaacata cctttctgag cgtagatggt gcaaatttcg acgactttct tggagtcttc
      541 tacggatgct ttttccacgc cttcgagctt aatgttctct ttgatgaact caaccgcacg
      601 atgcgcacca gttgtaactg cttgcgtgga cgcgccagag aaccagtaga tcaccgcgcc
      661 gccgacaatg agtccgagca ggaatggtgg atgcagaacc gagaggttcg caagcagacc
      721 aggctgcagg ccttttgtca gaatcacaat gattgagaaa atcatagtag ttgcgcccac
      781 gacagcggtt ccaatcagaa ctggcttcgc agttgctttg aaagtgttac ccgcgccgtc
      841 attcttctca aggaattctt ttcctttgat gaaatgagga tcgaatccaa attctttttt
      901 gatctcttcg cgagcgccct tgcgcgtttc gatgagagaa agttcaaata cagactgagc
      961 gttgtcagta accgggccgt aagagtctac agcgatggtt acaggaccca ttccaaggaa
     1021 accaaaagct accagaccaa aggcgaaaac agcaggagcc atcatgccga gcggtccttc
     1081 gattcccatg gtgctgattc catatgcgcc agccatcaga gctgcgatcg tgattcccat
     1141 ccagtatgcg ctgaagttac cagcagtcag accggaaaga atgttcagag atgcgccgcc
     1201 ttctttagaa gcagatacaa cttcgcgcac gtgaccggat tcagtagacg tgaacacttt
     1261 cacgagttct ggaatgatcg cacctgcgag tgtaccgcaa gtaatgatag aagaaagttt
     1321 ccaccagatc gtggtatcac caccgaggtt cgggatgagc acgtaagaaa caacatatgt
     1381 cagagcgaca gaaacgaaag aagtcagcca tacaagaact gtaagcggat gctcgtagtt
     1441 catgtcgtct gcgtttccgt aacgcgcttt cgcaatcaat gcgttgattc cgtaagaacc
     1501 ggaagaagcg aggatcatca caatgcgcat tacgaagatc caaacgagca gagatgcctg
     1561 aacggttggg tcttttactg caagcagaat gaaagaaatg agagcaacac ctgttacacc
     1621 ataagtttcg aatccgtcag cagttggacc aactgagtca cctgcgttgt cgcctgtgca
     1681 gtctgcaatc acacccgggt tacgggcatc gtcttcttta attttgaaaa cgatcttcat
     1741 gaggtcggaa ccgatgtccg cgatcttggt gaagataccg cccgcgattc tcagagcaga
     1801 tgctccaagt gattcaccga ttgcaaaacc gatgaagcaa ggaccagcgt attcagcagg
     1861 aatcaacaga agaatgcaga gcatcaggaa cagttcgatt gaaatcagga ggagaccaat
     1921 gctcatacct gctctcagtg gaattgcgta gttaggaaat ggcttaccgc gaagcgccgc
     1981 gaatgcggtg cgagagtttg caaacgtgtt gatacgaata ccaaaccatg caacaccgta
     2041 agaacctgcg ataccgatca agctgcaaag cagaatgacg agcaccgtag ttgccggcat
     2101 atggcgcagg aaaccaaagt aaaccaccat gattacgcca atgaaacctt cgagaaccag
     2161 gagcagtttg ccttgctgaa gcagatacgt tttacacgtt tcatagatca gctcggagat
     2221 ttcgcgcata gcgatgtgca ctggcagatt cttgatcgca gtgaactcca tcagaccgaa
     2281 cagcataccg agaacacaaa tacccatgcc tacgaaaagc agggtcttcc catttccgat
     2341 tgcaaagtta acggatgaca gatctggaat tttaagatcc gcttcgctcg caaaaatcga
     2401 gaagggagca aagaacgcta ctataaggag caggggcata tacttccgca gtacggaagt
     2461 tgtcttgcgc tgagcgatag acgagcccaa gccttttgag cctgaaactt tcttcatttt
     2521 gaacctctta agaaattgag taagagccga ttcggatctt acggatgcaa aatgtgcatt
     2581 ttaagattga ctcatcgatg cgcgaaaact gacaactcta atttgatggc agaaggcgga
     2641 atttccctat cgaatgcgtg gattttgccg tttgtgatct ttctgctctg cgttgccatc
     2701 attccactga tcaaccatca cttctgggat cgccatctat ggtggttttc tcttggtttc
     2761 ttttgttttc ccatggtcta cgctcttgtt ttcatcctgg gaggaccgtt cattcagaaa
     2821 acagttgaga aatgcatgga atatgtctca ttcatgattc tgcttgcgtc gttgtacgtg
     2881 atttcaggcg ggatcctgat tcgaggacac tttcgcggaa cacctctcgt caatacctgc
     2941 ttccttgtgg tgggttcggt atttgcaagt ttgattggaa caaccggtgc atccatgctt
     3001 ctgattcgtc cgcttttgcg cgcaaatgca gggcgaaagc ggacagccca tatagttgtg
     3061 ttttttatat ttacggtttc taacattgga ggcctgctca ctccactcgg tgaccctcct
     3121 ctgtttctgg gttatctgca gggtgttcct ttcctgtgga ccattcagct ctggaaagaa
     3181 tggttgttcg ttcttacact cgtgatcgcc ttattctggc tagtcgacgc gtacattctg
     3241 cgaaaggatg gaaagggaat cgatcttcat caccacgata caaccgagaa gtttcacata
     3301 tccggcgtgc tgaacctgat tcttctgtta ggagtggttg cttccatttt cttccacggc
     3361 tattttctgc gaacggtctc cggttggcct cactttgggc cgcaggaagg atccatgcta
     3421 gtgttaacgt gcgtctctct cctggcgatg ccagtgagcc atgagatacg cctgcaaaac
     3481 ggtttcagct ggaagccaat tctagaagtc atgtttctat tcgctgccat cttcgcgacc
     3541 atgattcctg cgctgcatat cctcgagacc agggcgacat cgctcggcat cagtgctccg
     3601 tggcagttct tcctcgcttc cggcctgttg tcttctctgc tagacaatgc gccaacgtat
     3661 ctcgcttttc tgtccgtcgc caaggggctc gcacttgcca acgatgctgg tttcgtattt
     3721 cacgatggca cgcatgtaag cgccgcgatt ctggctgcta tttcgtgcgg ttcggtgttc
     3781 atgggcgcaa acacttacat tggaaatggt cccaacttca tggtcagagc aattgcggcg
     3841 gaacgaggag tgaagatgcc cggcttcttt ggctacatgg gatattcagc cgccatcctt
     3901 ctcccgatct tcgcactggt tgcctggata ttcttctagt cgcttcgcaa cctttgtatc
     3961 tacaggatca cttctactcc tgcctgaagt ccgcccatcg tatccttcgc atggcttccg
     4021 ggataggtca gtgcagaatc aatgagcaag ttttgaaact ctgtagcggt tggctgagag
     4081 atgcgcgtta gacgcacttt gttgtctcga atagcggaag tctctgtgcg aagttgagca
     4141 aagatgcgaa tgttctctga aacagaatga aagtatcccg caatgaattc tacgccattt
     4201 acatgatagc cgccgtcttc gcgtcgaatg agcccgctcc cagagccacc cgtattgacg
     4261 tcagctgttt ctctattctg aaaccaggat ccatctgcag aaaaaagcgc aagcctgagt
     4321 ttgaagttcg atccgttctc aagatgataa cgatagtcgc caccgaagat caaaccagtg
     4381 cccactccgc gcgaacgaaa atcaaaactc tgcccgtata ctgatccaga gacagagaga
     4441 ccggactgcg aaattgtaaa cctctgggca tatttataat cgtaagtggc tcgagtgtcc
     4501 acgcgtctga ttcccccaag gatcgaaatc tcatgcgctg aatcttgcga tgttaaatcg
     4561 taacgtgcga gaagaaccgg agtaaatctg ctgagttcta cgtttgtcgt atcgtactgc
     4621 tgaacatcgt aacgaatcgt agacgaagtc aagctcacag cagaaacacc cgcatagtcc
     4681 gggcgagagt gaatggacgc tatttcaagc tcaaaatgaa acggatgggt agagtattct
     4741 gcttcgagcg gcaacccgga tgcattcttg ttgttccagg gtgcgttggc cccgatcccg
     4801 ccggccacaa aggaaccata gagacgatca ttcgcgtact tctcctgcaa atcaggagtg
     4861 tagcccccgc caaaaatcga cgcgcgaaga cgaagccctt ccggatctcg agccgcaagt
     4921 gccgtcgtgg tgagaaagaa acaaattgca agaagcctca tgagtggaat accgggcaaa
     4981 cggctttgcg cggtcaagcg atcgccggaa aagaccgcaa cgatcttgaa tcaaaatgac
     5041 accagaagca atcacaggat ttgaaaaccg tctgatccga atgcacaaac acattggcaa
     5101 gtgggcccgc agacagggaa ttacctgttt tcgcatctac gatcgtgatg ttcctcaatt
     5161 cccgctctgc atcgacaaat acgaagattt tatcatcgtt tcagaatata gaaccagcaa
     5221 gacggcagag ggagatgaac acgatcagtg gatcgacctg tgtcgtcaga tcatagcaaa
     5281 gactctgtct gttcccatcg cacatataga ttttcgg
//
LOCUS       DAOQJL010000236         5306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9010_length_5306_cov_5.131404, whole genome shotgun sequence.
ACCESSION   DAOQJL010000236 DAOQJL010000000
VERSION     DAOQJL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9010_length_5306_cov_5.131404"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..869
                     /locus_tag="PLG78_10995"
     CDS             132..869
                     /locus_tag="PLG78_10995"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNE23688.1"
                     /translation="MKLADLQKLFEENARGLSDELSSYIEPAGTLSAKDAVHVYRTGH
                     TVRMTEALGEIYEGCWRLLGDEEFFKAAEAYIAKYPSQSYNLADYGEHFAEFLADRFP
                     GQPVASELAQFERKFAQLFHAKEETGCTAEEIATMDESCRMQLVSSMFIQEGSGAFYS
                     IWQARANTDPPEINFDSPEMIVCCRSGTEILIQSISRAQCSILRSLMAGKTLAESLNL
                     SNATAEEVKELFAYLAANKLILKLERG"
     gene            complement(866..1420)
                     /locus_tag="PLG78_11000"
     CDS             complement(866..1420)
                     /locus_tag="PLG78_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNE23689.1"
                     /translation="MKTILFLGNSLTEGWGLFPGQSMPDLIQKKIQDSGLPYVHINGG
                     YRGDTSGQALSKLKRYLSLSPSGSVDVCVIELGTNDWMQGYSVADIESNLTTILATLR
                     SSNSAMVLALIEMRSFPGFRQGEEYDSLFSKFSKRNSIALIPFPLTAVITNDSLLQSD
                     GVHPNAKGTEIYADEVWKYLFPLL"
     gene            complement(1422..2942)
                     /locus_tag="PLG78_11005"
     CDS             complement(1422..2942)
                     /locus_tag="PLG78_11005"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNE23690.1"
                     /translation="MAEKIYIFDTTLRDGEQSAGVAFTGEEKLEIAFQLERLKVDIIE
                     AGFPCTSPGDLKAVQLISGEIKNATICALARAVPRDIDLAWEAVQHAKDPRIHVFINT
                     SDIQIAHQLKKGRDEVLIQAEAMVRHAAKYTSNVEFSPMDATRSDREFLYLMIERCID
                     AGATTINVPDSVGYAIPEELGGLFRSIMENVKNIHKCRISFHGQNDLGLCTANSLSAI
                     QNGVRQIECTINGIGERAGNTSLEEVVMAIKTRKDFLQFYTDIQTEEIYRASKLVEGL
                     SGMPVQWNKAIVGKNAFRHGSGIHQDGILKLRETWEIMNPAEIGIPKGTELVMGKLSG
                     RHAFKVHLEEMGYHLSEAETERVFNNFKELADKKMQVDDRDISALVQAHLGEPEKEAW
                     KLELVQVSAGDHAAPTATIRLIDSHGTLRTDAAVGTGPVDAIYQCINRITGLTPALTE
                     FSVKSVTEGIDAQGEVTIRIEESGRAFLGKASDTDIIVSSARAYLNALNRMIAARQ"
     gene            3077..4885
                     /locus_tag="PLG78_11010"
     CDS             3077..4885
                     /locus_tag="PLG78_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNE23691.1"
                     /translation="MNILSIDRVSKHIGDRSLFENLTFGIADDDRLGLIGPNGAGKTT
                     LLRILSGDSEPDDGRVAKSTQLQIANLPQIPVFQPDLSIRQALLSPISISNKRVTEDM
                     EREMVGILRELGMTDLDLRMDSLSGGMIKKVALAQTLALDADLLILDEPTNHLDAWTN
                     LWLEDVLRAWKKALLVITHDRYFLENVTNRILELEDGKLYQYEGNYSYYLEKKAEREA
                     SEARTQAKQSRLLKSELEWLRRQPKARGTKQKARVDRIEDLAANQRGGQERAFAFDSS
                     GTDSKNRILEVKGLGMSYGGRSLFSGFEHTFRSRERLGIIGPNGCGKSTFFNILMGKI
                     EPEAGTLKKGLHTKFGYFDQTSRDLDPNAKVLEYVKKNAGEIVGRADGSMTEATRLLD
                     YFGFSSRMQQGPIGKLSGGERRRLHLILMLMASPNFLILDEPTNDLDIQTLVLLEEFL
                     SDFQGCVLVASHDRYFLDRVAASLLVYENGKIEPYMGTASEYLAENRAHTSESENKKK
                     SAPARQETKPETDVAPKKSGRKLTPNEIKEYREIERQIEKLETDKKSVEESLSNPSSG
                     HEELKKLAESHAALEISLAEKWKRWEELSAIKDAAG"
     gene            complement(4899..>5306)
                     /locus_tag="PLG78_11015"
     CDS             complement(4899..>5306)
                     /locus_tag="PLG78_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23692.1"
                     /translation="RYLLFEWLGFYYRINPIRRSGDFKMSGPQIMNIQTGTNGPTSLS
                     IIVPVDYAQFSDKGIRHRLEASVRFFCRYYAVLGFLKEDYKRNYSIYVGNSFATIGGF
                     TPKTSGLGIGEMSQAHKASKMEVYLKFGAAFFL"
BASE COUNT         1422 a         1329 c         1296 g         1259 t
ORIGIN      
        1 gaactggcgg tcgccagatt caaagacgcg ccggtgctga tcgaatggga tgaaagcatt
       61 ccagaatttt ctgagctgga agcagaagta aatcgcgcac gcaaggtcgc tctcgccgcg
      121 gagagcgcga aatgaagctt gccgatctgc aaaaactatt cgaagaaaat gcgcgcggcc
      181 tgtcggatga gctttcgagt tacatagaac cggccggcac tctcagcgcg aaggacgcag
      241 tccatgtata tcgcacggga catactgttc gcatgacgga ggcgctcgga gagatatacg
      301 aaggttgctg gagactgctt ggagacgaag aatttttcaa agcggcagag gcatacattg
      361 cgaaatatcc ttcgcagtcc tacaatctgg cagactacgg agagcatttc gcagaattcc
      421 tggcagatcg atttccaggg caaccggtag cctctgagct ggctcaattt gaacgcaagt
      481 tcgcacagct ctttcacgca aaagaggaaa ctggatgcac ggcagaggaa atcgccacca
      541 tggacgaatc ttgccgaatg cagttggtat cctccatgtt cattcaggaa ggaagcgggg
      601 cattctattc tatctggcag gcacgtgcaa acacggatcc acctgaaatc aattttgatt
      661 ctcctgaaat gattgtctgc tgcagatcag gaacagagat tttgattcag agcatttcgc
      721 gcgcgcagtg ctccatcctg cggtctctca tggccggaaa gactctagca gaatctttaa
      781 atctttcgaa cgctaccgca gaagaagtga aagaattgtt tgcttatcta gccgcaaaca
      841 aactcatcct gaaactggaa cgcggttaaa gcagaggaaa caaatacttc cagacttcat
      901 ccgcgtatat ctctgtccct ttcgcatttg gatgcactcc atccgactgc agaagagaat
      961 cgtttgtgat gaccgcggtg agcggaaaag gaatgagcgc tatgctgttt ctttttgaaa
     1021 atttagaaaa aagagaatcg tactcttctc cctgtctgaa tccagggaaa gaacgcattt
     1081 ctatgagagc aagaaccatg gcagaattcg aagatctcag cgtcgcgaga atcgttgtaa
     1141 gattgctttc gatatctgcc acggagtagc cctgcatcca gtcgtttgtg cccagctcga
     1201 tgacgcaaac gtccacactt ccagaaggag acagagatag ataccgcttc aatttagaga
     1261 gagcctggcc cgacgtgtcc ccacgatagc cgccattgat atgcacgtag ggaagacccg
     1321 agtcctgaat ttttttttga atgaggtcgg gcatggattg ccctgggaac aggccccacc
     1381 cttccgtgag gctattgcct aaaaacagaa ttgttttcaa atcactgacg cgctgcaatc
     1441 atgcgattga gtgcatttag atacgcgcgc gcactcgata cgatgatgtc cgtatccgat
     1501 gccttcccaa gaaaggcacg accggactct tcaatgcgaa tcgtgacttc tccctgcgcg
     1561 tcaattcctt ccgttacaga cttcacgctg aattcagtca aagcaggtgt aaggcctgtt
     1621 atgcgattga tgcactgata gatcgcgtcg acagggcccg tccctacggc tgcatctgtg
     1681 cgaagagttc catgagaatc aatcaaccgg atggtggcag tcggtgccgc atgatcgccg
     1741 gcagaaacct gaaccaattc cagcttccag gcttctttct ctggttcacc aagatgcgcc
     1801 tggacgagcg cagagatgtc gcggtcatcc acctgcattt tcttatctgc gagttctttg
     1861 aaattgttga acactctttc cgtttccgct tcgctcagat gatatcccat ttcttccaga
     1921 tgcaccttga acgcatgacg acctgaaagc tttcccatta caagctcggt tcctttggga
     1981 ataccgattt ccgccgggtt catgatttcc caggtttcgc gcagttttaa aattccatcc
     2041 tggtgaattc cgcttccatg acgaaacgcg ttcttaccaa cgattgcttt attccattga
     2101 accggcattc cggaaagccc ttctaccagt ttgctcgccc gataaatctc ttctgtttga
     2161 atgtctgtgt agaactgaag aaaatccttt cgcgtcttga tggccatgac cacttcttcc
     2221 agagaagtgt tccctgctct ttctccaatt ccgttgatgg tgcattcaat ctggcgcact
     2281 ccattctgaa tggcggagag agaattcgca gtgcaaagcc caagatcgtt ttgaccgtga
     2341 aaactgattc tgcatttgtg aatgttcttc acgttttcca tgatggatct aaaaaggccg
     2401 ccgagttctt ctgggatcgc gtaaccaaca gaatccggaa cgttgatggt tgtcgcaccc
     2461 gcgtcgatgc atctttcgat catcagatac agaaactctc gatctgacct ggttgcatcc
     2521 attggactga attcaacgtt ggatgtgtac ttcgcagcat ggcgaaccat ggcctctgcc
     2581 tggatcagta cttcatcgcg accttttttg agctggtgag cgatttgaat gtcacttgta
     2641 ttgatgaaca cgtgaatgcg tggatccttt gcatgctgca ctgcttccca ggcaaggtca
     2701 atgtcacgcg gaacagcccg ggccagggca cagatggttg cattcttgat ttcccctgaa
     2761 atgagctgaa ccgctttcaa atctccagga gaagtgcacg gaaagccagc ctcgatgata
     2821 tctactttga ggcgttccag ttgaaacgca atctcaagct tttcttcgcc ggtgaatgcc
     2881 actcctgcgg actgctctcc atcgcggaga gttgtatcaa agatatagat tttttctgcc
     2941 atgccattcc agggaaatgt ccggcaagaa aagggcaagc gacatttgtt tttgccatgc
     3001 cacgggctcg cctttcactg gcagaggaag cgctttttcc agctgggcgc tttgcagatc
     3061 ggagccactg aactagatga acattctgtc tatagatcgc gtttcaaaac acataggcga
     3121 tcgctctttg tttgagaacc tgacgttcgg gatcgcagac gacgacagac tgggcctcat
     3181 tgggccaaac ggagcaggca agacgactct gcttcgcatt ctctccggcg actcagaacc
     3241 agacgatggc cgcgttgcga aatccacaca gctgcaaatt gcaaacctcc cgcagattcc
     3301 ggtcttccag ccagacctgt cgattcgcca ggcacttctc tctcccatct ccatttcaaa
     3361 caagcgagtc acagaagata tggaacgcga aatggttggg atcctgcgcg aactgggtat
     3421 gacggacctg gatcttcgca tggattcact ttccggcgga atgatcaaga aagtggctct
     3481 cgcacagact ctagcactcg acgcagattt gctgattctg gatgaaccaa ccaaccacct
     3541 ggacgcgtgg accaatctgt ggttagaaga tgtactcagg gcctggaaaa aagcactcct
     3601 cgtcatcact cacgatagat acttccttga aaacgtaacc aatcgcattc tggaactgga
     3661 agatggaaag ctttatcagt atgaaggaaa ttactcttac tacctggaaa agaaagcgga
     3721 aagggaagcg agcgaagcgc gcacgcaggc aaagcaatca cgtcttctca aatcggaact
     3781 ggaatggctg agaagacagc caaaagcgcg cggaaccaaa caaaaagcgc gcgtggatcg
     3841 aatcgaagat ctggctgcaa atcagcgcgg tggacaggag cgtgcgttcg cattcgactc
     3901 aagcggaaca gacagcaaga acagaattct agaagtgaag ggcctgggaa tgtcttacgg
     3961 cggccgcagc ctgttctcgg gatttgagca tacgtttcgc tcgcgcgaga gacttggaat
     4021 cattggaccg aatggttgcg gaaagtctac tttcttcaat attctcatgg gcaagatcga
     4081 accagaagcc ggcactctta aaaaaggact tcacacaaag tttggttact ttgatcagac
     4141 atcacgagat ctcgatccga acgcgaaagt actggagtac gtaaaaaaga acgcaggcga
     4201 aattgtggga cgcgcagacg gaagcatgac ggaagccacg cgtctcctgg actactttgg
     4261 attctcttcg cgcatgcagc aaggccccat tggcaaactg tcgggcggag aacgtagaag
     4321 attgcatttg atcttgatgc tgatggcttc tcccaacttt ctcatactcg atgaaccaac
     4381 caatgacctg gacatccaga ctctcgttct attggaagag ttcttgtctg atttccaggg
     4441 ctgcgttctg gttgcatctc acgaccgcta cttcctagat cgcgtcgccg cttctcttct
     4501 tgtatacgaa aatggaaaaa tagagcccta catgggcact gcaagcgagt atcttgctga
     4561 aaaccgcgcg cacacttcgg aatctgaaaa caaaaagaaa tcggcaccgg ccagacagga
     4621 aaccaaacca gaaacggatg ttgctccaaa aaaatccggc cgtaaactca caccgaacga
     4681 aatcaaagag taccgcgaaa tcgaaagaca aatcgaaaaa ctcgaaacag ataagaaatc
     4741 tgtagaggaa agcctgagca atccctcaag cggccatgaa gaactcaaga aactggcgga
     4801 gtctcatgca gcactcgaaa tcagtctcgc ggaaaaatgg aagagatggg aagagctctc
     4861 cgcaatcaaa gacgcggctg gctgattgcg cacaatcgct acaggaagaa agcagcaccg
     4921 aacttcaaat acacttccat tttgcttgct ttgtgagcct gagacatctc tccaattcca
     4981 agcccggaag tctttggagt aaagccacct atcgttgcaa atgagttgcc cacataaatg
     5041 ctatagtttc ttttataatc ttctttcaag aatcccagaa ctgcatagta gcgacagaag
     5101 aatcgaacag acgcttcgag cctgtggcga atccctttgt cgctgaactg agcatagtcc
     5161 acgggaacaa tgatggaaag agatgttggc ccatttgttc ctgtctgaat gttcatgatc
     5221 tgcggaccgg acattttgaa atcgccggat ctgcggattg gatttatgcg ataataaaag
     5281 ccaagccatt caaagagcaa ataacg
//
LOCUS       DAOQJL010000237         5298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9032_length_5298_cov_3.753385, whole genome shotgun sequence.
ACCESSION   DAOQJL010000237 DAOQJL010000000
VERSION     DAOQJL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_5298_cov_3.753385"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLG78_11020"
     CDS             complement(<1..251)
                     /locus_tag="PLG78_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNE23693.1"
                     /translation="MVVNCVLYSAGHPVQNIEINEIRSHLGQQDSFIWLGLYEPNEEL
                     MKEVQKAFGLHDLAVEDAHNAHQRPKIEAYGDSLFLVLHT"
     gene            343..1413
                     /locus_tag="PLG78_11025"
     CDS             343..1413
                     /locus_tag="PLG78_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23694.1"
                     /translation="MPAFDAIAINPRDTRALVIGADPEDVARTASFLGALSYQVEGVS
                     DYDRAVQKVEEFSPHIVLLDAVFNDLSGIDLLEQIKKARPDTGVILVSHAPRPEQILH
                     AFFEGASGFLTKPLFITEVDLRIRSVLKKIAYRKQMQETANHLEAEKQLLLRYFSGDV
                     AEKLLSGEITADLKGTRADVSMFFLSLRNAAAIAKTMSAGDFSHFLSQIFVDVLDLVY
                     SHHGSVNKLTGAGILATFGLPVASPNDAKNCVDCARSLQEHFQLMNETGAWPQRIEFG
                     IGIATGSVFAGNIGSYRKMEYTVIGDPANLAARLDQLSRKLGIDILADGATVARSGVQ
                     GAERIAESSVRGKKHDVEIFRL"
     gene            1410..2471
                     /locus_tag="PLG78_11030"
     CDS             1410..2471
                     /locus_tag="PLG78_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HNE23695.1"
                     /translation="MNLKMFRPDLARATILIVEDDPDQLALLEYYLALMRFRTAGVST
                     GADARVWLEQNQTDLILLDVNLPDMDGLVLLAEVREKNPDQAVMIMSAEKRAELIVEA
                     LFEGAEEFLQKPFSLIELSTRMHNILEMVAFRRNAESLVEKLSKEREILARYFSPEIV
                     ESILKQGPASQGGEYIHTTVLVYDLRNSTGLAEELGSERFAEIINMITVDLMDLIFSN
                     KGTITKLTGDGLIATFGTRESAEDVHNAVKCALAMREYYETILPVAMEQGVELAGFGI
                     GLATGRAFAGNVGSFRRMEYAIVGDVLSRAGRLEALTKSLGHPILVDARTQSFVPEVP
                     VMRRFLGRGRGKEAQLFGL"
     gene            2529..3761
                     /locus_tag="PLG78_11035"
     CDS             2529..3761
                     /locus_tag="PLG78_11035"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNE23696.1"
                     /translation="MNKIKVANPVVELDGDEMTRIIWKFIKEKLILPYLDVDIKYFDL
                     GVEYRDKTEDKVTVEAAEAIKKYNVGIKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNVPRLVPNWTAPICIGRHAFGDQYRATDFVTKGKGKLTITFTPEG
                     GAPQTHEVFDFKGDGVALAMYNTDESIRGFAYSCFNMALTKKWPLYLSTKNTILKKYD
                     GRFKDIFEEIYQKEFKKAFEAAGTVYEHRLIDDMVASALKWNGNFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYREHMAGKPTSTNPIASIFAW
                     TRGLEFRGKLDNNPDLVKFCQALEQVCVETVESGKMTKDLAVCVYGNKVNHGEHFLYT
                     EEFLSALDTNLKAKLGYK"
     gene            3800..4618
                     /locus_tag="PLG78_11040"
     CDS             3800..4618
                     /locus_tag="PLG78_11040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE23697.1"
                     /translation="MILTFPALDRIRSINSGAKITIAANPLYAQAALEFRLVDEILSF
                     EGRELRPLFIGQTANIHFDEAYVWMKDVDDLFVQSLRKICPVYASPSIQSHPFVHQSE
                     FLFRIHGADVSASEIARVWASLPGRLPIRSEEFDVCIHPGSAGIHKRWALERFRAVRD
                     SLVQSGRRVSWILGPAENELLESGIVMSGSEDTIFRSPSIRELIGILSSCKVFLGNDS
                     GVSHLAAAAGAPCVVIFGPTDPCIWAPRGNVQILHTLDASVQDVLDLLKSARRH"
     gene            4624..>5298
                     /locus_tag="PLG78_11045"
     CDS             4624..>5298
                     /locus_tag="PLG78_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE23698.1"
                     /translation="MILVVEDSPDQQRLVRQMLETAGYRDVRTCDDAPEALEIVRKNI
                     DQMEAVITDVWLIDGSGIQLTRDIRKIDQLRDLPVIIMTGKEGPEILPQAVEAGVNDF
                     LFKPFHWMELIARIRNNLRLKSEIARRVAREIELAAAKDELREALSRFEAVSHQDALT
                     GVSNRRKMDEHLEREWRLAQRARRPVSVVLIDVDYFKEYNDQYGHPQGDNCLRIIARV
                     VANGVHR"
BASE COUNT         1309 a         1274 c         1418 g         1297 t
ORIGIN      
        1 gtgtgtaaaa caagaaacag actgtctcca tatgcctcaa ttttaggcct ctggtgcgcg
       61 ttgtgggcat cttccactgc aagatcatgc agaccaaacg ccttctggac ttccttcatc
      121 aactcttcgt ttggttcata aaggccaagc cagatgaatg aatcctgctg cccgaggtgc
      181 gagcgtatct cgttgatttc tatgttctga accgggtgac cggcagaata gagcacgcag
      241 ttgacgacca tacagttcta tatcttcgca catcaggaag aaagcaatcg caaaaatcca
      301 ttacggcttg cggttttcgg cgcgcggcca ttcctgcgac ttgtgcctgc atttgacgca
      361 atcgcgatca atccacgtga tacgagggcc ctggtaattg gagctgatcc cgaggatgtt
      421 gcccgcactg cctcttttct gggtgcattg tcctatcagg tggaaggggt ttcggactac
      481 gaccgggcag tgcaaaaggt agaagaattt tccccgcata tcgtacttct cgatgcggtg
      541 tttaacgatc tcagcggaat cgatttgctc gaacaaatca agaaggcgcg cccggataca
      601 ggcgtaatac tcgtttcgca tgcgccaaga cccgaacaga tattgcatgc cttctttgaa
      661 ggtgcatccg gattcttgac caagcccctt tttattactg aggtggacct tcgcattcga
      721 agtgtgctga agaagatcgc ctatcgtaaa caaatgcaag aaactgcaaa tcatctcgag
      781 gcagaaaagc agctcctgtt gcgctacttc tccggggatg tggcagaaaa gcttctttct
      841 ggagaaatta ccgcggatct taaaggaacc cgggcagacg tttctatgtt ttttctcagt
      901 ttgcgaaacg cggctgccat tgcaaagaca atgagtgcag gcgatttctc tcatttcctc
      961 agtcagatat tcgtagacgt tctagatctg gtctattctc accatggttc tgtaaacaag
     1021 ctgacgggcg cggggatact ggcgactttt ggacttcctg ttgcatcccc aaacgatgcg
     1081 aagaactgcg tcgattgcgc tcggtctttg caggagcatt ttcaattgat gaatgagaca
     1141 ggagcctggc cgcagagaat tgaattcggg attggaatcg caaccggatc tgtctttgcc
     1201 ggaaacattg gctcttatcg taaaatggaa tacaccgtga ttggagatcc tgcgaatctg
     1261 gcagcgcgac tcgatcagct gagtcgtaag ctagggattg atattctggc tgacggcgcg
     1321 acggttgctc gttcgggagt gcagggcgca gagaggattg cagaaagttc agtgcgtggc
     1381 aagaaacacg acgtggaaat attccgtcta tgaatctgaa aatgtttcgg ccggatctag
     1441 cccgggcaac catactcatc gtggaagacg atccggatca actcgctcta ctggagtatt
     1501 atctggcttt gatgaggttc aggactgcag gagtttcgac cggagctgac gcgcgcgtgt
     1561 ggttagagca gaatcaaaca gacctgatcc ttttggacgt gaatcttccg gacatggatg
     1621 gccttgttct gctggcggaa gtgcgcgaaa aaaatccaga tcaggctgtg atgatcatgt
     1681 ctgcggagaa gcgagcggaa ttgattgtgg aagcattgtt cgaaggagcg gaggagtttt
     1741 tgcaaaaacc gttttccctc attgagcttt ccacccgtat gcataacatt ttggagatgg
     1801 ttgcttttcg tcgcaacgcg gaaagcctgg tggagaaact ttcgaaagaa agagagattc
     1861 tggcgcgata cttctctcct gagatcgttg aatcgatttt gaagcagggg cccgcttcac
     1921 aaggcggcga atacattcac acaacagttc tcgtttacga tctgaggaat tctactggcc
     1981 tcgcggagga gcttggctcc gagagattcg cggaaatcat caacatgatc accgtggatt
     2041 tgatggatct catattttca aacaagggca ccatcaccaa gctcaccggc gatggtttga
     2101 ttgcaacgtt cgggacgcgc gaatccgcag aagacgtcca caatgcggtg aagtgcgctc
     2161 tggcaatgcg cgaatattat gaaacgatct tgcctgtagc catggagcag ggggtagagc
     2221 tggccggatt cggcattggc ctggcgactg gccgcgcatt tgccggaaat gttgggtcct
     2281 ttcgtagaat ggaatacgcg attgtgggag atgttctttc gcgcgccggt cgcctggaag
     2341 ctctgacgaa gtctctgggc catccgatcc tcgtagatgc gcgaactcag tctttcgtgc
     2401 ccgaggttcc ggtgatgcgt cgttttctgg ggcgaggcag gggcaaggaa gctcagttgt
     2461 tcggactgtg aaggcattca tagaaaaagt cttgcccgcc cttagccctc acgcgcaaca
     2521 tcggaaacat gaataagatc aaagttgcca atccagttgt agaacttgac ggagatgaga
     2581 tgaccaggat catctggaag ttcatcaaag aaaaactgat tcttccatac ctggatgtag
     2641 atattaagta cttcgacctt ggcgttgaat accgcgacaa aactgaagac aaagtcaccg
     2701 ttgaggctgc ggaagccatc aagaagtaca acgttggcat caagtgcgcc acaattactc
     2761 cagacgaagc gcgcgtaaaa gaatttaatc taaagcaaat gtggaaatcg cctaacggca
     2821 caattcggaa cattctggat ggcactgttt tccgcgaacc tatcgtttgt aaaaacgttc
     2881 cgcgcctggt tccaaactgg actgctccca tttgcattgg ccgccatgca ttcggggatc
     2941 aataccgcgc gacagacttt gtaaccaaag ggaaaggaaa gctcaccatc acattcacgc
     3001 cggaaggtgg cgcaccccag acacacgaag tcttcgattt caaaggagac ggcgttgctc
     3061 tggcaatgta caacacggat gaatctatcc gcggctttgc gtattcctgc ttcaacatgg
     3121 cgctcacaaa aaagtggcct ctgtatctct ctacgaagaa tacaatcctg aaaaaatatg
     3181 acggaagatt caaagacatt tttgaagaaa tctatcagaa agaattcaaa aaggccttcg
     3241 aagcagccgg tacagtgtat gagcatcgtc tcattgacga tatggttgct tccgcgctca
     3301 aatggaatgg caactttgta tgggcttgca aaaactacga tggggatgtg cagtctgaca
     3361 ccgtcgcgca aggctttggt tctcttgggc tcatgacttc tgtgcttgta acgccggacg
     3421 gcaagaccat ggaagcagaa gcagctcacg ggaccgttac gcggcattac cgcgaacata
     3481 tggcaggcaa gcctacttcg accaatccaa tcgcatccat cttcgcatgg actcgtggtc
     3541 ttgaattccg cggcaaactg gacaacaatc cagatctggt gaaattctgc caggcgctgg
     3601 aacaggtctg cgtagagact gtagaaagtg ggaagatgac caaggacctt gctgtttgcg
     3661 tttatggcaa caaggtcaat cacggagagc atttcctcta cactgaagaa ttcttgtccg
     3721 cactggacac gaatctcaaa gcaaaactcg gctacaaata aaaacgatcc tcgcagtgcg
     3781 gcccggagct ctcggagacg tgatcctcac gtttcccgct ctggaccgca tccgttctat
     3841 aaacagcggc gcaaaaatta caatcgctgc aaacccgtta tacgctcagg cggcacttga
     3901 attccgtctg gtagacgaga tcctttcttt cgagggcagg gaactgcggc ctttgttcat
     3961 tggtcagacg gcgaatattc actttgatga agcgtatgtc tggatgaagg acgtggatga
     4021 tttgttcgtt cagtcgcttc gcaaaatttg tccggtgtat gccagccctt ctatccaatc
     4081 gcacccattt gttcatcagt ccgaatttct ttttcgcatt catggtgcgg acgtgtccgc
     4141 gtctgagata gccagggtct gggcatcact gcccggtcga ctgcctatcc gatcggaaga
     4201 gttcgatgtt tgcattcatc caggatccgc tgggatccac aagagatggg ccctggaacg
     4261 gttcagggcc gtaagggatt ccctggtaca aagtggccgc cgcgtatcct ggattctggg
     4321 cccggcagag aatgaactcc ttgagtctgg cattgtcatg tccgggagtg aagataccat
     4381 cttccgaagt ccttccatac gggagttgat tggaatcctt tcctcctgca aggtcttcct
     4441 tggaaacgat tcgggtgttt ctcacctggc tgcggcggcg ggtgcaccct gtgttgtgat
     4501 ttttggtcca acggatcctt gcatctgggc tcctcgtggc aatgtgcaga tcctgcatac
     4561 tctggatgct tctgttcaag acgtgcttga cctcctcaag tccgcccggc gccattagaa
     4621 agcatgatcc tggttgtaga agattcgcct gaccagcaga gactggtgag acaaatgctg
     4681 gagacagccg gttacagaga tgttcgcaca tgcgacgatg caccagaggc ccttgagata
     4741 gtgcgcaaga acatcgatca gatggaggca gtgatcaccg acgtctggtt gattgatgga
     4801 agcggcattc aattgacccg agacattcgc aaaatagatc agctgcggga tctccctgtg
     4861 atcatcatga ctggaaagga aggtcccgag atcttgccgc aggcagtgga agcgggagtc
     4921 aacgattttc tatttaagcc atttcactgg atggaactga ttgctcgcat tcgaaataat
     4981 ctgagattga aatctgaaat tgcgcggcgt gttgcgcgag agattgagct tgctgcggcc
     5041 aaagatgaat tgcgcgaagc cctcagccgc tttgaggcag tttcgcatca ggatgctctt
     5101 acaggcgtct ccaatcggcg aaagatggac gaacacctgg aaagagaatg gcgcctggct
     5161 cagagagcgc gtcgtcctgt aagcgttgtg cttattgatg tagattactt taaggaatac
     5221 aacgatcaat acgggcatcc ccagggagac aactgtcttc gcattattgc ccgggtcgtt
     5281 gccaacggtg tgcatcgc
//
LOCUS       DAOQJL010000238         5292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9044_length_5292_cov_3.682643, whole genome shotgun sequence.
ACCESSION   DAOQJL010000238 DAOQJL010000000
VERSION     DAOQJL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9044_length_5292_cov_3.682643"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLG78_11050"
     CDS             complement(<1..636)
                     /locus_tag="PLG78_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23699.1"
                     /translation="MRAADKDLTTEEIRYYHSLLQEELTDFDCGTLCAPDNNGTPYCC
                     KSEHAVPLLYKAEFELLTSMGDLWHVWKPVTSEDREIASTASRDQIFCECKGIEHCIR
                     DQRSISCRTFPLEPYLDRRGVFVGLTFVRDFTEKDPDTGKVKCPMTRRKKEIRQAFVD
                     MHFMFWEKILLRRLDEYETYIDTSKRLRRERNRTGRQFHVLFPSHFLDSKTV"
     gene            716..2044
                     /gene="miaB"
                     /locus_tag="PLG78_11055"
     CDS             716..2044
                     /gene="miaB"
                     /locus_tag="PLG78_11055"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004768679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNE23700.1"
                     /translation="MAESRKVYLETYGCQMNEYDSGLVRKILEGSYSAVGEPEQADLI
                     LLNTCAIREKAHEKVYSRLESFHILKRKRPDLVIGVLGCMAQNLGEDLFSQGLPLDFI
                     AGPDNYRNLPELIESSQSGSSERVHTRLSQDETYGDIQPTVTNGVLASVTIMRGCDNF
                     CSFCVVPYTRGRERSRSIDSIVEEVSRLIEEEGVREVMLLGQNVNSYKYEGHDFAALV
                     ERLLSSTSIARIRFTSPHPHDFPDHLLSLMASEERFCSQIHLPVQSGSTSVLSRMKRD
                     YSREEYLALVAKMKDRIPEVGLSTDIIVGFCGETESEFEDTLSLVKECRFDVAYMFRY
                     SEREGTFAKKKMPDDIPEEVKLERLARLISVQSESSLAKNQEEIGKVHRVLVEGRSKR
                     SAADNSGRSDSGKKIIFPAAGTKPGEIVPVRIESVTSATMRGTVVAPVFS"
     gene            2079..3065
                     /locus_tag="PLG78_11060"
     CDS             2079..3065
                     /locus_tag="PLG78_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23701.1"
                     /translation="MNRLISILLFLWLAPLLADRDVHGDISSAEVSRYLDVRLDLDRL
                     AVQPGQKVMVRYRVTNQGYRIFRIFPHEDARRTFQFLVEDKAGKEVAPRERPEDASEQ
                     PVRDLVGNRVKEILLHPGETFEKTMDLNAMYDLSPGQEYRVCAYFVPDQESDLAIRSK
                     NWARVRVSKMERAPAAREQDAAVSSDSGLSPEETVYLFLSAEIKHNWPAYLKHVDLPR
                     FVGSYDRFALKYAQASDLERPGVLREFSTYLTRRDGDDLKRFRILRTDLDRDKSGEIL
                     PTGNARVTVQADRDTEGFRVRYEYVYTLKRAEQEGFWKIIHVSARVVRQGQS"
     gene            3118..4143
                     /gene="fliM"
                     /locus_tag="PLG78_11065"
     CDS             3118..4143
                     /gene="fliM"
                     /locus_tag="PLG78_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNE23702.1"
                     /translation="MTEILSQDEIDALLSAISTGEVEMEDYSNVGEQKKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVGVHVGSVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAVLEIDPGITFTIIDRLFGGPGEPTKITRELSDIEMSVMESIIVRI
                     LGNLREAWATVIDLRPRLGNIETNPQFAQIVPPNDMVVLITLETKVGDVEGMTNLCIP
                     YITIEPIISKLSAQYWYSSIRRGESDENRAIIQERLDMVDIPLRCEVGEVDLPMADVI
                     NVREGDVVRLGGTHIKSDMMIRVGDRKKFKCRPGRIGNRLCVQIGDAIEDVPDELLGS
                     SRSEDEV"
     gene            complement(4140..4622)
                     /locus_tag="PLG78_11070"
     CDS             complement(4140..4622)
                     /locus_tag="PLG78_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23703.1"
                     /translation="MRAKMKTRPGILIDEKDRWIYQQAELIQPDILAYFRQNLHRSDR
                     TYFIRNTFGELVEEGELDAVLGFPLHAISAVPQTEDVHFLVDSGERITIPFSAIYLDR
                     DDCFFFEMPGRGVPVRLTASAMTHVSDTLVDTSPPRWSAGVPVSRKSLSEYFINPPAR
                     "
     gene            complement(4607..5008)
                     /locus_tag="PLG78_11075"
     CDS             complement(4607..5008)
                     /locus_tag="PLG78_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23704.1"
                     /translation="MRRKILFAAIFALASCRSVEFIPDAEYANRRKDNPESFHLYARK
                     PDVKVEALGRLIFRNFYGNPGSEEFQRDVALEAAARGANCGYVSKKQINHQTQFRTNA
                     IDSQNKHGGVTGEVQAEIETVEVTLFQCERK"
     gene            5030..5278
                     /locus_tag="PLG78_11080"
     CDS             5030..5278
                     /locus_tag="PLG78_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23705.1"
                     /translation="MAKKGSKESSQGIITYIRTLAPLAYDVFTAWRDRPKWEQIMADL
                     ELVAQENEKLRKRSVLLTVFLGALFLWNAVLTYFLFAR"
BASE COUNT         1309 a         1272 c         1397 g         1314 t
ORIGIN      
        1 cactgtcttt gagtccagga agtggcttgg aaacagtacg tgaaattgtc tgccagtgcg
       61 attgcgctcg cggcggagtc tcttggaagt atcaatatag gtttcgtact cgtccaggcg
      121 gcgaagaagg attttttccc agaacatgaa gtgcatatcc acgaacgcct gacggatttc
      181 tttttttctc cgcgtcatgg ggcattttac cttgcctgtg tccggatctt tttcagtgaa
      241 atcgcgcacg aaagtgagac ccacgaaaac cccgcgccgg tccaggtacg gttcgagtgg
      301 aaaagtcctg catgaaatgg atctctggtc gcgaatgcag tgctcgattc ccttgcattc
      361 acagaagatc tggtctctag aagcagtgct tgcgatttca cggtcttccg aggtgactgg
      421 tttccaaaca tgccagagat ctcccataga agtgagcagt tcaaattctg ctttgtatag
      481 aaggggcact gcatgctccg atttgcagca gtagggagtt ccattgttgt ccggagcgca
      541 cagtgtgccg cagtcaaagt cagtaagctc ttcctgaagc agagaatggt agtagcgaat
      601 ctcttccgtg gtaaggtctt tatccgctgc ccgcattatg tctctatctc tctcgaacgg
      661 ttccctgtaa attaagatag caacgggtgc gagctttttg ttctttggac ggaacatggc
      721 ggaatcgcgc aaagtgtatc tggagacgta tggctgccag atgaatgaat atgattcggg
      781 tctggtgcgt aagatcctgg aaggaagcta ttctgccgtg ggtgaaccag agcaggcaga
      841 tctcatctta ctcaacacct gcgccattcg cgaaaaggcg cacgagaagg tctatagccg
      901 cctcgaatcc tttcacatac tcaaacgcaa acgcccggat ctggtcatcg gagtgctggg
      961 atgcatggca cagaatctag gagaggattt gttttcgcag ggtcttcctc tggatttcat
     1021 cgccggcccg gacaattacc gcaatctccc tgaattgatt gagtctagcc agagcggaag
     1081 cagcgagcga gtgcacacga ggctatccca ggatgaaacc tatggcgaca tccaaccaac
     1141 cgttacaaat ggagttctgg catccgtaac catcatgcgc ggttgcgata acttctgttc
     1201 cttttgcgtt gttccttaca cgagagggag agagagaagc cggtcgatag attccattgt
     1261 ggaagaagtc agcaggctga tcgaagaaga gggcgtgcgc gaagtcatgc ttcttggcca
     1321 gaatgtgaac tcctataaat acgaaggcca tgattttgcg gctcttgtgg aaaggctcct
     1381 ttcttctact tccattgcgc gtatccgctt tacttctcct catccgcatg actttccgga
     1441 tcatcttctg tccttgatgg catcggaaga gcgcttctgc tctcagattc atctaccggt
     1501 tcaatccgga tccacctctg ttctttctcg catgaaacga gattattcgc gcgaagagta
     1561 tctggctctg gttgcgaaaa tgaaagacag aatcccggag gtcggactga gcacggacat
     1621 cattgttggc ttctgcgggg aaacggaatc tgagttcgag gataccctca gtcttgtgaa
     1681 agaatgcaga ttcgacgtcg cttacatgtt ccggtattcg gagcgcgaag gtacgtttgc
     1741 caaaaagaaa atgccggacg atattccgga agaggtcaag ctggaacgcc tggcccggct
     1801 catttccgtt caatccgaaa gctcccttgc aaaaaaccag gaagaaatcg gcaaggtgca
     1861 tcgggtgctg gtggaaggca ggagcaagcg atctgcagcg gacaactcag gcagatctga
     1921 ctcgggtaag aagatcattt ttccggcagc cggcacgaag cccggggaaa ttgtgcccgt
     1981 ccgtattgaa tcggtcacgt ccgcaaccat gcgcggaacc gtagttgctc cggttttttc
     2041 ctgaaatttt tccggagaag atcgatacta aagatagcgt gaatcggctc atcagtattc
     2101 tcttattcct atggctggct ccattgctcg ccgaccgtga tgttcacggg gatatttcgt
     2161 ctgcagaagt gtcgaggtac ctggacgtgc gtctggacct ggatcgactg gctgttcagc
     2221 cggggcagaa ggtaatggtg cgatatcgcg tcacaaatca gggttatcga atctttcgca
     2281 tttttcctca tgaagacgcg cgccgcacgt ttcagtttct ggtagaagac aaggccggaa
     2341 aggaagtggc tccgcgcgaa aggcccgaag atgcatccga gcaacctgtg cgtgacctgg
     2401 tggggaatcg agtaaaagaa atccttcttc atcctggcga aacgtttgaa aagactatgg
     2461 acctgaatgc aatgtatgat ctgtctccgg gtcaggaata tagagtgtgt gcctatttcg
     2521 tgccagatca ggaatcagat cttgccattc gctcaaaaaa ctgggccagg gtgcgagtga
     2581 gtaagatgga aagagctccg gccgccagag aacaggacgc agcagtgagc tctgattcag
     2641 gcctttctcc ggaagaaaca gtctaccttt ttctgtccgc tgaaatcaaa cacaactggc
     2701 ctgcctatct caagcatgta gatttgccca ggttcgtggg atcgtatgat cgttttgctt
     2761 taaaatacgc tcaggcatcc gacctcgaga ggccgggagt gcttcgcgaa ttttctacgt
     2821 atctgaccag gcgcgacggc gacgacctga agcgttttcg catcctgcgt acggatctgg
     2881 atcgagacaa gtccggggaa atccttccca ctggaaatgc cagggtcacc gttcaagcgg
     2941 atcgcgacac ggaagggttt cgtgtccgct acgaatatgt gtacactctc aaaagagcag
     3001 agcaagaggg gttctggaaa atcatccatg tatctgctcg agtggttcgt cagggccaga
     3061 gctgaaaaag gattgccaca atggttccac acgttcaatc tgagatgagg ccggcgcatg
     3121 actgagattc tttcccagga tgaaatagat gctctacttt ccgcgatttc cacgggcgaa
     3181 gtggaaatgg aagactacag caatgtaggc gaacaaaaga aagtaaagat ctatgacttt
     3241 cgcagaccgg ataagttctc aaaagaccag attcgtactc tgcagatgat gcatgaaaca
     3301 tttgcccgtc tgactacaac cgcgctttcc gctcagctgc gcgcactcgt cggtgtgcac
     3361 gttggttccg tggatcagct gacgtacgaa gaattcattc gatccattcc gaatcctacc
     3421 acacttgctg taatcaacat ggatccattg aagggttcag ccgttcttga aatcgacccg
     3481 ggcatcacat ttaccatcat agaccgattg tttggtggtc caggggaacc tacgaagatt
     3541 acgcgggaac tttcagacat cgagatgtct gtaatggaaa gtatcattgt gcgcatcctc
     3601 ggaaacctca gggaagcgtg ggccaccgta atcgatctta gacccagact cggaaatatt
     3661 gaaacaaatc ctcaattcgc gcagattgta ccgcccaatg acatggtggt tttgatcaca
     3721 ctggaaacaa aggtgggaga cgtagaggga atgaccaacc tctgtatccc atacattaca
     3781 atcgaaccaa tcatttcaaa actgagtgcg cagtactggt attcatccat tcgccgcggt
     3841 gagtcagatg aaaacagggc catcatccag gaacgtctgg atatggtgga cattcccttg
     3901 cgctgcgaag ttggagaagt ggatcttcct atggcggacg tcatcaatgt acgcgaaggg
     3961 gatgtggttc gcctcggtgg cactcacatc aaatcagaca tgatgattcg ggtgggagat
     4021 cgcaagaagt tcaaatgccg tccaggtcga atcgggaatc gtctgtgtgt tcagatcggg
     4081 gatgcaattg aggacgtgcc ggacgaacta ctaggatcaa gtcgttcaga agacgaagtc
     4141 taacgcgccg gcggatttat aaaatattcc gaaagggact ttcgagagac cggaacgcct
     4201 gcgctccagc gcggtgggct tgtgtctacg agagtatctg atacgtgagt catggcgctt
     4261 gcggtcaggc gaaccggaac tcctcttcca ggcatttcaa agaagaagca atcgtcccga
     4321 tccagataaa tggcagaaaa ggggatggtg attctctcgc cggagtccac aaggaaatgt
     4381 acatcttctg tttgcgggac agcggatatt gcgtgaagag gaaatccaag aaccgcgtcg
     4441 agttctcctt cttccactaa ttcgccaaat gtgtttcgaa tgaaatacgt gcgatcggac
     4501 ctgtgtaaat tctggcgaaa gtaagcgagg atgtcaggct gaatcagttc tgcctgctgg
     4561 tagatccagc gatctttttc atcgatgaga atccctggcc tggttttcat tttcgctcgc
     4621 attgaaacaa tgtcacttcc actgtttcta tttctgcctg aacttcccct gtaactccgc
     4681 cgtgcttgtt ctgactgtct attgcattgg ttctaaattg ggtctggtgg ttgatctgct
     4741 ttttagacac atatccacaa ttggctcccc gcgcagcggc ttccagggcc acgtctctct
     4801 ggaattcctc tgagcctgga tttccgtaaa aattgcgaaa gatgagacgt ccgagcgctt
     4861 ccactttcac gtccggctta cgggcataca gatggaatga ctcaggatta tctttgcgcc
     4921 tgtttgcata ttcagcatcc ggaatgaatt caaccgatct gcaggacgcg agcgcaaata
     4981 ttgcggcgaa tagtatttta cgccgcacgc caggcccgca gaatgctcca tggccaaaaa
     5041 agggtcgaaa gaaagttctc aaggcatcat cacctatatc agaacactgg ctccacttgc
     5101 atatgacgta tttactgcgt ggagagatcg tcccaaatgg gaacaaatta tggccgatct
     5161 agagcttgtg gcccaggaaa acgagaagtt gcgcaaacga tccgttctgt taacagtgtt
     5221 ccttggcgca cttttccttt ggaatgcggt gttgacatac tttcttttcg cgcgctgaaa
     5281 gagcattgct tt
//
LOCUS       DAOQJL010000239         5277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9081_length_5277_cov_4.851589, whole genome shotgun sequence.
ACCESSION   DAOQJL010000239 DAOQJL010000000
VERSION     DAOQJL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9081_length_5277_cov_4.851589"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..458
                     /locus_tag="PLG78_11085"
     CDS             96..458
                     /locus_tag="PLG78_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE23706.1"
                     /translation="MQSHLDQTFSALADPTRRAMIAHLSRGEASVSELARPFLKSMSL
                     PAVTKHIKVLEKAGLVTKTREAQWRPCRLNAAALRDVADWTHQYQKFWEESFDRLEQY
                     LKTAKVKENKKGKKHGKS"
     gene            445..897
                     /locus_tag="PLG78_11090"
                     /pseudo
     CDS             445..897
                     /locus_tag="PLG78_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            922..1410
                     /locus_tag="PLG78_11095"
     CDS             922..1410
                     /locus_tag="PLG78_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE23707.1"
                     /translation="MNRTFEAPRDRMFEMWINPEHLSQWMGPAGSTMKLIRSDIRKGG
                     GIFYSMSGDNGTMYGKANYLEIDRPHKLVYTQHFCDEQENVSRHPMAPTWPETMLTRV
                     VFEEESGDRTRVTVIWEVYGSATPEEVATFINGRSGMTQGWGGSFDRLDEYVASSGVK
                     VA"
     gene            1731..1967
                     /locus_tag="PLG78_11100"
     CDS             1731..1967
                     /locus_tag="PLG78_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE23708.1"
                     /translation="MLTRRATLPACYFTFPLLTTTPESYNGRYRVAEAERLMKAHPEK
                     TLIEIAFESGFNSKTSFHVGFKKLRGVAPSKFGR"
     gene            1971..2753
                     /locus_tag="PLG78_11105"
     CDS             1971..2753
                     /locus_tag="PLG78_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23709.1"
                     /translation="MSRYTEELKKKAARLQESDAKRRAVWLSVNQWVERLFQKLANII
                     ERQNSKKNDRFLVWWRIRTIDPVVGMFVSSALITLLILTFTIPISEYQTMLLAGSTGD
                     RIALAAGVAGYIVWFWGIYDAVRFKYWRRRCPFEIRGWENLVDDPELEHERWRPCEIH
                     ITLAIDSPDIRAGLTALYDVFAIEANRLYYNADSEDDGKKRIPWMSSGTDATGSANSR
                     VLWKIYKLLSEHLGKFHRKTKAIASVSITAGASFNVSHPSSD"
     gene            complement(2868..3332)
                     /locus_tag="PLG78_11110"
     CDS             complement(2868..3332)
                     /locus_tag="PLG78_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNE23710.1"
                     /translation="MEPKGTRPGLTAMKLKNKIFLGLLALGSVAFFANCRHHSIEDRS
                     KWVTGKISDKLDLDKSQEAQLDKIRDEVVAKVKAQKPQRDQLAQEFQTLVKADKLDKT
                     KLKDLQTKHDALHAEIENLVIDRMAELHASLKPEQREKAANAIQKLRDHMAR"
     gene            3391..4566
                     /locus_tag="PLG78_11115"
     CDS             3391..4566
                     /locus_tag="PLG78_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE23711.1"
                     /translation="MIYFALFFVYFLGNGAYFSGFSPYVLGNYPAESAAIFLSSQIAS
                     PAGTLLAGVTTDKTRLLRWPLFVCVSSLVVFQFLFFSVLSPLWTIVSSICLRFFLSAA
                     GQILVIATLESGGARFARARTAGTVGFCVIQIVLYLLAETLVAHSSAEFGRWTAAFYS
                     IGMILALFVPLKRKTHEPYYFSEAVQRMFRGSAPAFFVASFFFYLCYQLVDYYIGRFL
                     EMRHGISAVYLGWGLSVFLEILLMPFTALLFEKFHTRSLFVLAIAAGALRFLWLGVAA
                     TSEIPAVFAQLLHGLHFAGYYAGVIFLLRRYFPAHLYGTGHALFSGFAMSCGGIAGNM
                     LTAYMLGKSPGVSSFAIVFLGAAGIHILLIGAFYFMRLPGGAVSHSARTEAENPPAV"
     gene            complement(4569..>5277)
                     /locus_tag="PLG78_11120"
     CDS             complement(4569..>5277)
                     /locus_tag="PLG78_11120"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23712.1"
                     /translation="FAMTDELRRVCEEIQQRIGDRLFHETALGNRPALESLVEEYWWI
                     RLRRTTHAWKKNTLPPVITHNLVDDAHDDVLNQIRYLSMFNAQSDRVKVIYHPDFLSE
                     TNPLFGMDYEQFSRGCHLGIFPGSYEPWGYTPLECLVRGVPAVTSDLAGFGSYVKQNI
                     PSHQNSGLYVLARRGVEFQNASTELTAILENYCRLERRDRIVLRNRCEALSDRFDWMD
                     LASAYFEAHGMALSRTK"
BASE COUNT         1227 a         1270 c         1360 g         1420 t
ORIGIN      
        1 ccgtcactgt gaagaagggg attcgtgacg ggtcaaaacc cgattgacaa gctttctgca
       61 tacaaccgat tggttaatta acctataggt atattatgca gagccacctt gaccaaacgt
      121 tttctgcgct cgcggacccc acacggagag ccatgattgc tcacctctcc cgtggtgagg
      181 cgagcgtatc tgagctggcc aggccttttc taaagagtat gagtctccct gcagttacca
      241 aacacatcaa ggtccttgaa aaggcggggc tggttacaaa aaccagggag gctcaatggc
      301 gtccctgcag actcaatgca gcggcactga gggatgtggc ggattggacg caccagtatc
      361 aaaaattctg ggaagagagc ttcgatcgtt tggaacaata tttgaaaaca gctaaggtta
      421 aagaaaacaa gaagggaaaa aaacatggaa aaagctaaca agtctaacgc gattagcatc
      481 acccgggctt atgaggctcc gctgaaggcg gtgtgggacg cctggacgga tccaaaacaa
      541 attgcgcaat ggtggggccc aagaggattc actatcacaa cacacagcag ggacttgagg
      601 gttggcggta cctggcgtta cacaatgcac ggcccagacg gagtggatta tccaaacgta
      661 actcaatacc ttgaagtaga aaaatatcag aaactcgttt acgatcacgg tggagacgat
      721 actcgcgcgc ccatgtttcg tgtgacggtt ctgtttagcg aaacgggagg taagacaaaa
      781 atggaaatga ccatgagcct tgcaactcct gaagatctag cggaagcccg cgcaatcatt
      841 aaacaggctg gaggaaattc cacctgggat cgtcttgcgg aattccttga gaaagaatca
      901 actacgaagg agatatttgt catcaatcgc acctttgaag cacctcgtga cagaatgttt
      961 gaaatgtgga tcaatccgga acatttgtca cagtggatgg gcccggctgg ttctaccatg
     1021 aagctgattc gatccgacat cagaaaaggc ggaggcattt tctattctat gtctggtgac
     1081 aacgggacta tgtatggaaa agccaactac cttgaaattg atcggccaca taaacttgtc
     1141 tatacccagc acttctgcga cgaacaagaa aacgtctccc gtcatccaat ggccccgacc
     1201 tggcctgaga ccatgctaac gcgcgttgta tttgaagaag agagtgggga ccgcactcga
     1261 gtaactgtaa tctgggaagt ttacggaagc gccacgcctg aggaagttgc aacattcatc
     1321 aatggaagaa gcggcatgac ccagggatgg ggaggctcgt ttgatagatt ggacgaatac
     1381 gtcgcaagtt cgggcgtaaa ggtggcttga gtattcaaca ggcgggattg tcagactttg
     1441 gtttctagaa ttctgacaat cctgtagtgg atttgattga gcctgtcctg gcatggaccc
     1501 gttagttcga atcatgtagc ggtagctctt tttccgttcg gtgttataac ccggggcgac
     1561 aaccgcttct gagtatggta aaaatagaat tgctttgcga tcagttgaag agcaaatctc
     1621 gaatccatcc cgggttgaaa cacgcccggg ctttcccaga aaatacgcga tgctctgctg
     1681 catcgttgcg ctctgtctcg gatgctgcaa aatgggaggc gattgcatcg atgctgaccc
     1741 gtcgtgcgac gctgccagct tgctatttta ccttcccctt gctcacaaca acgccggaat
     1801 cttacaatgg acgctaccgg gtagccgagg ctgaacgact gatgaaagca catccggaaa
     1861 agactctgat cgaaattgcg tttgagtctg gtttcaattc aaagacatct ttccatgttg
     1921 gttttaagaa attgcgcgga gtagctcctt ctaaatttgg tagatgatct atgagccgct
     1981 atacggaaga gctaaaaaag aaagccgcac gcctacagga gtcggacgcg aagcgcaggg
     2041 cagtctggct ctcagtgaac cagtgggtgg aacgtctatt ccagaaactc gcgaatatca
     2101 tcgaacggca aaactcaaag aagaatgatc ggttccttgt ctggtggagg attcgcacga
     2161 ttgatcccgt cgttggaatg ttcgtatcgt cagctctcat cactcttttg attcttacct
     2221 tcacgatccc catatccgaa taccaaacaa tgttgctcgc gggaagtacg ggggatcgca
     2281 tcgctcttgc tgcaggtgtg gcaggatata ttgtgtggtt ctggggcatc tacgatgcgg
     2341 ttcgctttaa gtactggcgc agacgttgtc cattcgagat tcgcgggtgg gaaaacctgg
     2401 tagatgatcc ggagcttgag catgaaagat ggagaccgtg cgagattcat atcactctgg
     2461 caattgattc gccggacata cgtgccggcc ttaccgccct ttacgatgtt ttcgcaatcg
     2521 aagccaatcg cctttactac aatgcggatt ctgaggacga tggaaaaaag cgaattccgt
     2581 ggatgagttc aggtaccgat gcaacgggct ctgctaattc gcgcgtcttg tggaagatct
     2641 acaagctgtt aagtgaacac cttgggaaat ttcaccgcaa aacaaaagca atcgcttccg
     2701 tgtcgatcac ggctggagca tccttcaatg tatcgcatcc cagttcggat tgagatcctg
     2761 ctttttttca ttcgcgctcg ttttaaccgg cgtggcgaaa aaaatcccgg cagtgttact
     2821 gccgggtgct ttgatggtct tacgcgtttg caagcctcac attgcactca tcgtgccatg
     2881 tgatcgcgga gcttttgaat cgcatttgct gctttctcgc gctgctctgg cttcagagaa
     2941 gcgtgaagtt cagccatacg atcaatcacg aggttttcaa tttcggcatg aagtgcatcg
     3001 tgttttgtct gaagatcctt caactttgtc ttgtctagtt tgtcagcttt caccagagtc
     3061 tgaaattctt gcgcaagctg gtcgcgctga ggtttttgag ctttcacctt cgctacaact
     3121 tcgtcgcgga ttttgtccag ctgagcttcc tggcttttgt ccagatccag tttgtctgaa
     3181 atctttccag tgacccattt gcttctgtct tcaatgctat ggtgtctgca atttgcgaag
     3241 aacgccacag agcccagggc aagcaggccg aggaatatct tattctttag cttcatggcc
     3301 gtaagacctg gcctggttcc tttcggttcc atttgtgtct ctgtgaacat acgattgcat
     3361 gctctgcttg ccaaatttct ctgacatgca ttgatctact tcgcgctttt ttttgtttac
     3421 tttctgggta acggagctta tttctctggc ttcagcccgt acgtgctggg caactatccg
     3481 gcagaaagcg ccgccatttt tctttcgtca cagattgcat cgccggcggg aactttgctt
     3541 gcgggcgtga caacagacaa gacgcgtttg ctccggtggc cgctatttgt ttgcgtttct
     3601 tccctggtgg tttttcaatt cctgtttttt tctgtactat ctcccctgtg gacaatcgtg
     3661 agttccatat gcctgcgttt ctttttgtcc gctgcaggac agatcctcgt gattgcgaca
     3721 ctggagagcg gcggagctcg atttgcacgc gcgagaactg caggaacggt tggtttttgt
     3781 gtgatccaga tcgttctcta tcttcttgca gaaacgttag tcgctcattc gagtgcggaa
     3841 tttggccgct ggacagccgc gttctattca atcggaatga ttttagcgct ctttgttcct
     3901 ctcaagcgca agacacatga gccgtattat ttttcagaag cggtgcaaag gatgtttcgc
     3961 ggaagcgctc ccgctttttt tgttgcatcc tttttcttct atctgtgtta ccagcttgtt
     4021 gactactaca ttggacgatt ccttgagatg cgtcatggga tttctgccgt gtatctgggc
     4081 tggggccttt ctgttttcct tgaaatatta ctgatgccgt tcacggccct gctatttgag
     4141 aagtttcata cccggtcatt gttcgtactc gccattgcgg cgggtgcgct tcgttttctg
     4201 tggctcggtg tggccgctac ttccgaaatt cctgcggtct tcgcacaact cctgcacggc
     4261 ttacattttg cgggctacta tgccggagtg attttcctgc tgcgcagata cttccctgct
     4321 catctgtacg gaacaggcca cgctctcttt tccgggtttg ccatgagctg tggaggaatc
     4381 gcgggcaaca tgctcactgc atatatgctt ggaaagtcgc caggtgtgtc ctcgtttgcg
     4441 attgtttttc ttggcgcggc gggaattcac attctcttga ttggtgcttt ctatttcatg
     4501 aggctgccgg gcggcgctgt atcgcacagc gcgcgaacag aagcagaaaa cccaccggcc
     4561 gtttagtctt atttcgtgcg cgaaagagcc atcccgtgag cttcaaaata agcggaagcc
     4621 agatccatcc agtcgaatcg atccgataag gcttcgcatc gatttcgcag gacgatgcga
     4681 tcacgacgtt ccaatctgca atagttctca agaatagctg tcaattctgt ggaagcattt
     4741 tggaattcga cgccgcgtct tgcgagaacg taaaggccgc tattctggtg cgatgggata
     4801 ttctgtttta cataggatcc aaacccggcg aggtcacttg ttacagcggg aacaccgcgc
     4861 acgagacact ccaggggagt gtagccccac ggctcatagg atcctggaaa tattcccagg
     4921 tggcagccgc gggaaaactg ttcgtagtcc attccaaaca aagggttagt ctcgctgaga
     4981 aaatctggat ggtagatgac ctttacgcga tccgactgag cgttaaacat actcagatat
     5041 cgtatctggt tgagaacgtc gtcatgagca tcgtctacga gattgtgtgt tataacagga
     5101 gggagtgtgt tcttcttcca ggcatgcgtt gttctacgca agcgaatcca ccagtactcc
     5161 tccacgagcg attccaatgc tggcctgttg ccgagcgccg tttcatggaa gagacgatct
     5221 ccgatccttt gctggatttc ctcacagact cgtctgagtt catccgtcat cgcgaag
//
LOCUS       DAOQJL010000240         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9175_length_5242_cov_5.094081, whole genome shotgun sequence.
ACCESSION   DAOQJL010000240 DAOQJL010000000
VERSION     DAOQJL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_5242_cov_5.094081"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLG78_11125"
     CDS             <1..708
                     /locus_tag="PLG78_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE23713.1"
                     /translation="RGLFDFSHTRTDKLADSYDEALYHGARLSDLKGFKTVINSVSAT
                     TGHPFYFEGGNAKARLGEYETGFVSADSFSVARAVAASGAYPVFLSPLELTSSEYSPA
                     RTFLVDGGVYDNLGIHSLLREEDLDLLIVSDAGAPLRRNETPGDGVFAIESTMDIMME
                     QMRGMQIQRLTHLEKGKRAIWFSIDSKGEGDNAEICSRIPTGLSALPRNDLSILQRHA
                     ETLLRARLSAYAPEWIS"
     gene            918..1715
                     /locus_tag="PLG78_11130"
     CDS             918..1715
                     /locus_tag="PLG78_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE23714.1"
                     /translation="MARLFSLPFLLYAAFLAALCVDVVRLGDLRLAHGLLVASLSLLK
                     GRHQLVQTGDVIREWFDSLESITKAFGACYDSKPLESEGLCRIVQSSLEEVEKLRKIK
                     NQNIFCETPPSVAVLAEEKGLALCVRELLTNAFKYSPEGSNIHITSYRTGNSVSLVVL
                     NDVEFSTGRNTGVPREMENAVFEPFFRIHNTYDERFRSEELGMGIGLTVVQNAVNQFG
                     GSVYVYEITDHASGLVPRRRVAAELILRTSQEASIADRTVSMRSVTR"
     gene            complement(1661..3895)
                     /locus_tag="PLG78_11135"
     CDS             complement(1661..3895)
                     /locus_tag="PLG78_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE23715.1"
                     /translation="MLPARRSRAWSEFLGVFFSLSLVLITCAVQWAFWQYIQPTRWLL
                     FFPAMLFSALRWRAGGYTSTLGAATLVAIFFMPHTDTFPFIGPHDLLLSVIFTGGGLV
                     TSYTLNAARYIRMELKFHSEVLTNLAEGVMIVDTHNSKILFTNPRFDDLFGYSAGELL
                     GQHVSILNSKSNGAPRDRAQEIDDQLRKDGKWSGEVLNAKKDGTPFWCSVRITTFSPN
                     TADEVWIAVHDDITAAKAASEALALAERKFSDLAETIDEVFWMVNKDKTQMLYVSPAF
                     ERIFERTCASLYQNPLEWIECIHPDDRRRVLESAMNQVSSGHYAEDYRIVRPDGQIRW
                     IRDTGFTRKNRNGEVIGFAGVARDITDQKLMAEQILHSQKMESIGTLASGVAHDFNNL
                     LGIIMGQTAWLEENTQGETRRRIESIQQTAERGAALVRQILTFARKGETLRRSVDINA
                     LLLEFRPLISQLFPKNIHTEFHLDQNLPPLSLDSTQLHQVVMNLCVNARAAMPEGGDL
                     IVSTRLVDQSEVRTHFIHAEAKQYVEVSVKDTGSGMKEEIRSRIFEPFFTTRPGGEGT
                     GLGLAVASGIVESHSGFIRVESEPGKGSIFSVYLPVPLAPGRRENPNTAPEKATSTTA
                     NHTILVVEDEPHLLEIMKSVLSSHGYEVITAENGNAGLQLFRNGQFHAVVSDLSLPLM
                     DGYALAREMKRIAPSIPIIIASGFFDPILKLELEGAGIKHFLQKPYRVTDLIETVRSA
                     IEAS"
     gene            4068..4754
                     /locus_tag="PLG78_11140"
     CDS             4068..4754
                     /locus_tag="PLG78_11140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23716.1"
                     /translation="MNCPKCSANMEIRAIDGVEIDLCTGCGGIFLDDGEFQSFTGMDP
                     ATGFVKLSKFVKVLAKLNERAVLDELTGVYTRKYFNEYMETLFASEKRGKITLIGIDV
                     DHFKTVNTEFGHDGGDAVLRTVAQRLKDCMRSSRDDSVFRIGGEEFCVVLLELSPDDS
                     YHSAENLRRLVQMDPIPMPNNGEKVITISMGVALARPTDTPESLYKRADELLYEAKNS
                     GRNRVVMEKA"
     gene            4754..>5242
                     /locus_tag="PLG78_11145"
     CDS             4754..>5242
                     /locus_tag="PLG78_11145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE23717.1"
                     /translation="MGDVLNHYEDHLADIYSWIYGDFHARLRANHDFFEMHGIVGPGN
                     AIDLGAGPGYQSIALCDLGFSVTAADFSAKLLDELHERAGQRNIQTLQADILEDVWFG
                     RSPSLIVCMGDTLTHLPSQDAVSSLLDSIYGELGPGGRMILTFRDLKGTPGEIRFIPV
                     RSS"
BASE COUNT         1257 a         1240 c         1384 g         1361 t
ORIGIN      
        1 cgtggcctct ttgatttttc gcatacccgt acagacaaac tggctgattc atacgatgag
       61 gcactctatc acggcgctag actttcggat ctaaaaggat tcaaaacagt catcaattct
      121 gtgagtgcaa cgacgggtca tcctttctac tttgaaggtg gaaatgcaaa ggcgcggctg
      181 ggtgaatacg aaactggttt tgtaagcgct gattcgtttt ctgtggcacg tgcagtggct
      241 gcaagcggag cgtatcctgt atttctgtct cctctggagc ttacctcaag cgaatattcg
      301 cctgcgcgca ctttcctcgt cgatggtgga gtgtatgaca acctgggaat tcattctctt
      361 cttcgcgagg aagatctaga tctattgatt gtgagcgatg cgggcgctcc tcttcgaaga
      421 aatgaaacac cgggggatgg tgtgtttgcc attgaaagca cgatggacat catgatggaa
      481 cagatgcgcg gcatgcagat ccagagattg actcatctgg aaaagggaaa acgtgcgatc
      541 tggttttcta tcgatagcaa aggagaaggc gacaacgcgg agatctgttc tcgaattcca
      601 accggccttt ctgctctccc tcgcaatgat ctttctattt tgcaaagaca tgcagaaact
      661 ctgcttcggg cccggctatc cgcctacgct cccgaatgga tttcctgagt ggccagagtc
      721 tgatcactga acggctacgt ggattgcaat gagtgcggcc gaaaggatca accctgcgag
      781 gaagaacttc aagtccatgg agtacgtttg catgaagcca gattgtctgc tctatgagga
      841 tttgtccgta aaaatacctg gtcacactgg tacgtctacc ccttgacccg gaaaacaaat
      901 gtgataatct tccggggatg gcccggcttt tcagtttgcc attcttgttg tacgccgcgt
      961 ttctcgccgc gctgtgcgtg gatgtagttc gtctgggaga cctgcgtctg gctcacggtc
     1021 tactcgtggc ttccctgtca cttctcaaag gaagacatca actggttcaa acgggagatg
     1081 tgattcgcga gtggttcgat tctctcgaaa gcatcacgaa agcctttgga gcttgctacg
     1141 attcaaaacc actggaaagc gaaggacttt gccgcattgt ccaaagctct ttggaggaag
     1201 tggaaaaact gagaaagatc aagaatcaga atatattttg cgaaacacca ccctccgtgg
     1261 ccgtacttgc ggaagagaaa ggacttgctc tgtgcgttcg cgagcttttg accaacgctt
     1321 ttaagtattc gcctgaaggg agcaacatcc acatcacttc ctacagaaca ggaaattcag
     1381 tttcgctagt ggttctaaac gatgttgaat tttccacggg cagaaacacc ggtgtgccgc
     1441 gcgaaatgga aaatgctgta ttcgaaccct tctttcgcat tcacaataca tatgatgaac
     1501 gctttcgctc cgaggagctg ggcatgggca ttgggttgac tgttgtgcaa aacgccgtca
     1561 atcaattcgg aggcagcgtc tacgtctatg agatcactga tcatgcgtcc ggtctggttc
     1621 caagacggag agtggcagcg gaattgattc tgagaacttc tcaagaagct tctatcgcgg
     1681 atcgtactgt ttcaatgagg tcagttacgc gataaggttt ctgtaagaag tgcttgattc
     1741 ccgctccctc cagttccagt ttcaggattg gatcaaagaa tccgcttgca atgatgatgg
     1801 gaatagacgg tgcgattcgt ttcatttctc gagccagagc gtatccgtcc atcaaaggca
     1861 aactgagatc agatacgacc gcgtgaaatt gtccattccg aaacaattga agccccgcat
     1921 ttccattttc agccgtgatc acttcgtatc catgcgatga aagcacactc ttcatgatct
     1981 caagcaggtg aggctcgtct tccacaacga ggatagtatg attcgcggtt gttgaagtgg
     2041 ccttttcggg cgcagtgttt ggattctccc gtcttccagg tgcaagaggt acaggcaggt
     2101 acacagagaa gatgctccct tttccaggtt cgctctcaac gcgtatgaag ccggagtgag
     2161 attcaacgat gccggaagct actgccagtc caagtccggt cccttctccg cccgggcgag
     2221 tggtgaagaa gggctcaaag atcctggaac gaatctcctc tttcattcca gaccccgtgt
     2281 ctttgacaga gacctctaca tattgcttag cctcggcatg aatgaagtga gtgcgcacct
     2341 cgctctggtc cacaaggcga gtggatacaa tgagatctcc gccctcgggc atcgcagcgc
     2401 gagcgttgac gcatagattc atgactacct ggtgaagctg cgttgaatcg agcgagagag
     2461 gaggcaggtt ctggtcgaga tggaattcgg tgtgaatgtt ttttggaaag agctgactga
     2521 taagcggccg aaactcgagc aacaaagcat tgatgtccac agatcggcgg agagtttcgc
     2581 ctttgcgtgc aaaggtgagg atttgtctga ccagcgctgc gcctctttcc gcagtttgct
     2641 ggatggactc aattcgcctt ctggtttctc cctgtgtatt ttcttccagc caggctgtct
     2701 gacccatgat gatgcccaga agattgttga agtcgtgagc tactccgctt gcaagtgtgc
     2761 caatgctttc catcttctgc gaatgtaaaa tctgttctgc catgagtttt tggtccgtga
     2821 tatcgcgagc gacgcctgcg aaaccaatca cttctccgtt gcgattcttg cgagtaaatc
     2881 cagtgtcgcg gatccagcga atttgtccgt ccggtcttac gattctgtaa tcctctgcgt
     2941 agtgaccgga tgagacttga ttcatcgcac tctcgagcac gcggcgtcga tcatctggat
     3001 ggatgcactc tatccattcc aacggattct gatatagact tgcacatgta cgctcgaaga
     3061 tacgttcgaa tgcggggctg acgtatagca tttgagtctt gtctttgttt accatccaga
     3121 agacttcatc gatggtctct gccagatccg aaaattttct ctccgccagc gccagagctt
     3181 ctgacgcagc cttcgcggcc gttatgtcat cgtggactgc gatccagact tcgtctgccg
     3241 tgtttggtga aaatgtagtg atgcgaacag aacaccagaa gggcgttccg tctttcttcg
     3301 cgttcagtac ctcgccgctc catttgccgt cttttcgcag ttgatcgtct atttcctggg
     3361 ctcgatcccg cggggcacca ttgcttttcg agttgaggat gctaacgtgc tggccgagca
     3421 gttctcctgc gctgtatccg aataggtcat caaagcgggg attggtgaaa agaatcttag
     3481 aattgtgcgt gtccacaatc atgactcctt ccgccagatt ggttaagact tcgctatgaa
     3541 acttgagctc cattcttatg tagcgtgcgg cgttcagagt gtaggatgta accagacctc
     3601 ctcctgtgaa aatcacggag agcaagagat catggggacc aatgaatgga aatgtgtccg
     3661 tgtgcggcat gaagaaaatg gcaacgagcg ttgctgcgcc aagagtagaa gtatagcccc
     3721 cggccctcca cctgagagcg gagaagagca tggcaggaaa gaagagaagc catcgcgttg
     3781 gctggatgta ctgccagaac gcccactgaa ccgcacatgt tatcagaacc aggctcaggc
     3841 tgaagaatac gccaagaaac tcggaccagg ctcgagaacg gcgcgcaggc agcattgata
     3901 ttgcataacg tcacctgctt gatccgcaag caaattaatc gaccccgggc tggaattgaa
     3961 gctgactgaa cgcttcacag tatgcgcaaa aggttctgtt tggcttgatg gaaatgccag
     4021 tggttcttta gtcgtttatt gtaagtgcgc tcgctcagga gagttttatg aattgtccaa
     4081 agtgttctgc caatatggaa atacgcgcca tcgacggagt ggaaatcgat ctttgtaccg
     4141 gctgcggagg gatctttctg gatgacgggg agtttcagag tttcaccgga atggacccgg
     4201 ccaccggttt tgtgaagctc tcaaagttcg tgaaggttct tgcaaagtta aacgaacgtg
     4261 ctgttctcga tgaacttaca ggcgtgtaca cacggaagta cttcaatgaa tacatggaga
     4321 ctttgtttgc cagcgagaaa aggggtaaga tcactctgat cggaattgat gtagatcatt
     4381 tcaaaacagt aaatactgag ttcggacatg atggtgggga tgcagttctt cgcactgtcg
     4441 cccagagatt gaaagattgc atgcgctcaa gtcgcgatga cagtgtcttt agaattggtg
     4501 gagaagaatt ctgcgttgta cttcttgaac tgagcccgga tgatagctat cattctgcag
     4561 aaaacttgcg tagacttgtt caaatggacc ccataccaat gccgaataac ggtgaaaagg
     4621 taatcacaat ttcaatgggc gttgctcttg cgcggccaac ggatactccc gagtctcttt
     4681 ataaacgtgc ggatgaactt ctctatgaag caaagaactc ggggcgaaat cgcgtggtga
     4741 tggaaaaagc ttgatgggag atgtactcaa tcactacgaa gatcatctgg cggatattta
     4801 tagctggatc tacggagact ttcatgcgag gttgcgcgcc aatcatgatt tctttgaaat
     4861 gcacggaatc gttggacctg gaaatgcgat agatctcggg gcaggtcccg gttatcaatc
     4921 catcgcactg tgcgatcttg gtttttcagt gacagcagcg gacttcagcg cgaaactcct
     4981 ggacgagctt catgagcgag caggccagag aaacatccag acattgcagg cggacattct
     5041 cgaagacgtc tggtttggtc gctccccttc tttgattgtg tgcatggggg acactctcac
     5101 tcacttgcct tcacaggatg ccgtcagctc tcttctagat tccatttacg gtgaactggg
     5161 gccaggcggt aggatgatat tgacctttcg cgatttaaag gggactccag gggagattcg
     5221 ctttatcccg gtgcggagtt ct
//
LOCUS       DAOQJL010000241         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9177_length_5242_cov_3.513399, whole genome shotgun sequence.
ACCESSION   DAOQJL010000241 DAOQJL010000000
VERSION     DAOQJL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_5242_cov_3.513399"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLG78_11150"
     CDS             complement(<1..199)
                     /locus_tag="PLG78_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNE23718.1"
                     /translation="MGLSATSARRDVIANNIANVDVPNFKRSEISFEADLKRAVDSER
                     SFAEQPLLKTLHPGHIDGRRPI"
     gene            316..699
                     /locus_tag="PLG78_11155"
     CDS             316..699
                     /locus_tag="PLG78_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23719.1"
                     /translation="MKSLSRILPTPSARSLALILLAALAAGSCRSVYRNHDPAVPSTQ
                     TATLSQSGFVFRLTALKNKVSMSDTLSLKPGDYMIDFQLPSTGDGGVARCVLDAGKVY
                     KFKIVERRYLPEIGMHALVGECAIK"
     gene            686..1678
                     /locus_tag="PLG78_11160"
     CDS             686..1678
                     /locus_tag="PLG78_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23720.1"
                     /translation="MRSSNRSLRKVTRGLAFVLIACGIAEATLRFIPGVDFYLKENSL
                     HCAASFYLRLCPSRSIEFKNSAGETYTVTTNEAGERITSALRDTSRQDSRTTQLELWL
                     IGDSIAMGHGLTDSDTAAWIAQSRLKSWKVRNLATDSVGPLAMTDILKQTPGKPAQII
                     WIFNPSDFIDDPLETARRESFPRRAIFILKYYVRSSAIASAARIIAGSSDASYVQGGS
                     SPLPDAADPIFAHILELSKEAGNHGARFCILLYSDVEAGANKPEKENRLRSMIADLAL
                     AHHICTIDVRGQFQSSKENLYLQNGHPGPAASRIFADAIVNSVSGQEPRAKSEQ"
     gene            complement(1626..2393)
                     /gene="pssA"
                     /locus_tag="PLG78_11165"
     CDS             complement(1626..2393)
                     /gene="pssA"
                     /locus_tag="PLG78_11165"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNE23721.1"
                     /translation="MNIRAGLNWVPNLFTLGNLTLGFFAILFSAREGDSAGSLALSCG
                     LILLACLCDGLDGYAARLLKAQSDLGAQLDSLADLTAFGIAPAVVMYSFALRGINPVV
                     EGITLPIGMALSAVYPACAAYRLARFNVAHDPTSFQGLPSPVAGVIVALMPLGLQGSF
                     QIPVALMVVIFVGVAFLMVSTIKYSKPQATVFKRFNPVRLGILIAFIVIALIAVGILR
                     SFAISAALLFMIVLVYGISGVIALILHAVLGQRQNSQ"
     gene            complement(2390..3388)
                     /gene="tsaD"
                     /locus_tag="PLG78_11170"
     CDS             complement(2390..3388)
                     /gene="tsaD"
                     /locus_tag="PLG78_11170"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNE23722.1"
                     /translation="MFGIGIETSCDETSVAVIEDGLIMRSCAIYSQVADHARFGGVVP
                     EIASRSHLEKINSVFAQAMDDSGVRMEDLSYCAVTTRPGLVGSLMIGGILAKSIHLAF
                     GTPIVTCDHLEAHFYAAALDAGKPLVYPFLGLLLSGGNSAIFIVRGPGDLEQIADTAD
                     DAIGEAFDKTAATLGLSYPGGPAVESCASGYVEKSPSIFTPLLRNEQGLLFSFSGIKT
                     AVTKAKREGHDFAKICHDFQETCFELVTRMLTRAVKQTGIDQVVASGGVIANTILRSR
                     LESAAASGKFDLRYPAKKILCTDNGAMIGALGFFLFQKGKKDVVGFAVSDRRTQNP"
     gene            complement(3392..4756)
                     /locus_tag="PLG78_11175"
     CDS             complement(3392..4756)
                     /locus_tag="PLG78_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE23723.1"
                     /translation="MNLKVAALILCMAIAPAIAQNAPPSRGEKTSQAQIEASHMYQNV
                     LQQVLYLIENYHVDSVSRAKLFEGAVRGMLEATGDPYSRFLNREEQKDFNHSEGGDLV
                     GIGVEITIQGGYPLIVAPMRGGPAEKAGLMTGDRIISIGGQPTRYVGVSKLSELISGE
                     PGSIAELGIQRGGSSFPVRVERGVFQLEYVRTAFLADSRVGYVRLQAFFGEDAGSISK
                     FKKAVQDFARQNVKGIIIDLRNNTGGHVDMARTLSGYFLKKGDVVYRVKSRTDSREFV
                     ADGDTELVPPATKVVILVNRGSASASEILSGCLQDHGRAILVGEQTFGKASVQQVFRP
                     LPDETAVLVTTQRYLTPKNRAIHGIGLKPDVEVPDLMPTPQESVYLGSIEEKKMLEAF
                     LKENPQFDPSLVDKFAKRIAAENMSLRPEIVRLLLKREYGIREPDPDTQSDPQLRRAL
                     ELIQ"
     gene            complement(4753..5019)
                     /locus_tag="PLG78_11180"
     CDS             complement(4753..5019)
                     /locus_tag="PLG78_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23724.1"
                     /translation="MKKILMVLLLAALSGCYAKPTEADADRVIQEFMTLRFQAPLNPE
                     LSQKTDKEIFELACRSKRVKCDLVKDIVRTRNPELYKTLAGDKP"
     gene            complement(5016..>5242)
                     /locus_tag="PLG78_11185"
     CDS             complement(5016..>5242)
                     /locus_tag="PLG78_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23725.1"
                     /translation="AFIGEVGLTGEVRSPGAVSARIRELSSLGYKTIVLPEAAAKEVG
                     KGGAKEKSVTLRAMQQITELGAVVGAREEG"
BASE COUNT         1296 a         1429 c         1262 g         1255 t
ORIGIN      
        1 cgatcgggcg ccttccgtca atgtggcccg ggtgcaaagt cttgagcaaa ggctgctctg
       61 cgaaggatct ctctgaatcc acggctcttt tcaggtctgc ctcgaaggaa atctcgcttc
      121 ttttgaagtt cggaacgtcc acattggcga tgttgtttgc gatcacatct cgcctcgcgg
      181 aggttgcaga aaggcccatt tcgagcatct gcatacttct catgcctggt ccgtcaaaca
      241 tcgacatatt tagactttcg gccggtatat aaaaagcctt gatgaattat gtcacccgcc
      301 cgacattttt catagatgaa atccctttcc agaatacttc ccaccccttc tgcacgctcc
      361 ctcgctctga tccttctggc agccctggcc gcaggaagct gcagaagcgt ctacaggaac
      421 cacgatcctg ccgttcccag cactcaaacc gcgacgctgt ctcagtccgg cttcgtgttc
      481 agactgactg cgctcaagaa caaggtttct atgtcagaca ccctcagtct gaagcccggg
      541 gattacatga tcgatttcca gcttccttcc acgggagacg gtggagtggc cagatgcgtt
      601 ctggacgcgg gcaaagtgta caagttcaag attgtagaaa ggagatacct gcccgaaatc
      661 ggaatgcatg ctctcgtcgg cgaatgtgcg atcaagtaac cggtctttgc gaaaagtcac
      721 gcgcggcctg gcattcgttc tgattgcatg cgggatcgcg gaagcaaccc ttcgattcat
      781 tccgggcgtg gatttctatc tgaaagaaaa ttcgctgcac tgcgccgcct ctttttatct
      841 cagactctgc ccttccaggt cgattgaatt taagaactcc gccggggaaa catacactgt
      901 aactacaaac gaagccggag agagaatcac ttccgcattg cgcgacacat cgcgccagga
      961 cagccgaacg acccagcttg aactatggtt gatcggagat tccattgcaa tgggacacgg
     1021 cctcactgat tcagatacgg ccgcctggat cgctcagtcc agattgaagt cgtggaaggt
     1081 cagaaatctg gccacggatt ctgttggacc actggctatg acagacattc taaaacaaac
     1141 tccagggaag cccgctcaaa taatatggat ctttaacccg agcgacttca tagacgatcc
     1201 tctcgaaacg gcacgacgcg aatctttccc tcggagggct atctttattc ttaagtacta
     1261 cgtaagatca agcgcaattg catccgccgc gagaatcatc gctggaagtt cagatgcttc
     1321 gtacgttcag ggtggatcct ctcctctccc ggacgcggca gatccgatct tcgcacacat
     1381 tctggaactc tcgaaagaag ctggcaatca cggggctcgt ttttgcattc tgctttattc
     1441 agacgttgag gcaggcgcaa ataaaccgga aaaagaaaat cgtttacgat ctatgatcgc
     1501 ggatctggct ctggcacacc atatctgcac gatcgacgtg aggggtcagt ttcaaagctc
     1561 aaaagagaat ctgtatttac agaacggaca ccccggcccg gctgcttctc gcattttcgc
     1621 ggatgctatt gtgaattctg tctctggcca agaaccgcgt gcaaaatcag agcaatgact
     1681 ccgctgattc cataaaccag aacaatcata aacaacaacg ccgcagatat ggcaaagctt
     1741 ctcagtatcc ccactgcaat cagtgcaatg acaatgaagg cgatgaggat tccaagacgc
     1801 actggattga atcttttgaa taccgttgcc tgcggcttag aatacttgat ggtgcttacc
     1861 attaagaagg caactcccac aaaaatcaca accatcagag ccactggaat ttgaaaggag
     1921 ccctgcagac caagaggcat gagtgccaca atgacgccgg cgacgggaga tggaagtccc
     1981 tggaaagaag ttggatcgtg tgcgacgttg aaccgagcaa gcctgtaggc ggcacaggca
     2041 ggatacaccg cagacagagc catgccgatg ggtagagtga ttccctccac gaccggattg
     2101 attccgcgca gtgcgaaaga atacatcaca acggccgggg caattccaaa tgcagtcaaa
     2161 tccgcaagac tgtccagctg cgcgcccaga tcagactgag ctttgagcaa acgagccgca
     2221 tatccgtcga gcccgtcgca aaggcaggca agcaggatca aaccgcaaga cagagcaaga
     2281 gaaccggcgc tgtccccttc tctggcggaa aacaagatgg cgaagaatcc cagtgtaaga
     2341 ttgccgagag tgaataggtt cggtacccag ttgaggccag cgcggatgtt catggattct
     2401 gagttcttct gtctgacact gcaaatccca caacatcttt tttgcctttc tgaaacagaa
     2461 agaatccaag cgctccgatc atcgctccgt tgtccgtgca gaggattttc tttgccggat
     2521 atctcagatc gaatttgcca ctggctgccg ccgactcaag gcgcgatcgc agaatcgtgt
     2581 ttgcaatcac tccgcccgag gcgacgacct ggtcgattcc ggtttgcttg accgctcttg
     2641 taagcatccg cgttacaagt tcgaaacagg tctcctgaaa atcgtgacaa atctttgcga
     2701 aatcgtgtcc ttctcgtttt gcttttgtga ctgcggtctt gattcctgaa aaagaaaaca
     2761 ggagtccctg ctcgtttcga agcagaggag taaaaattga cggactcttt tcgacgtatc
     2821 cggatgcgca agattccacg gccggcccgc ccggatagga aagacccaaa gtggctgccg
     2881 tcttatcaaa tgcttcgccg atcgcatcat cagcggtgtc tgcaatctgt tccagatcgc
     2941 ctggccctct cacaatgaag atggcggaat tcccgccgct caatagaagt ccaagaaacg
     3001 gatacacaag tggtttgccc gcatcgagag ccgcagcata aaaatgcgcc tctagatgat
     3061 cgcacgttac gatgggcgtg ccaaaagcaa gatgtataga ttttgcaagt attccgccga
     3121 tcatcaaaga tccaacaagt cccgggcgag tggtcacagc gcaataggac agatcttcca
     3181 tgcgaacacc ggaatcatcc attgcctgcg cgaagactga attgattttt tcaagatgcg
     3241 atctgctggc gatctctgga accactccgc caaacctggc atgatccgcg acctgagagt
     3301 agatggcgca cgatctcatg atcaggccat cttcaatgac tgctactgac gtttcgtcgc
     3361 aggacgtttc aatcccgatg ccgaacatga actactgaat caactcaagc gcacgacgaa
     3421 gctgcggatc gctttgagtg tctggatccg gttcgcgaat tccatactct ctctttaaga
     3481 gaagacgaac gatctcaggg cgaagagaca tgttttcagc agcaattcgt tttgcgaatt
     3541 tatccacaag agaaggatca aactgtggat tctcttttaa gaaggcttcc agcattttct
     3601 tttcttcgat cgaccccaga tacacggact cctggggcgt gggcatcaag tcaggtactt
     3661 ccacatctgg cttgagacca attccatgaa tggctctgtt ttttggggta agatatctct
     3721 gtgtagtgac aagaactgca gtttcatccg gaagaggacg aaacacctgt tgcacggacg
     3781 cctttccgaa tgtttgctct ccgaccagga tggctcttcc atgatcctga aggcagccgg
     3841 acagaatctc agaggcggat gcagaccctc gattgaccag aatcacaact tttgtcgcgg
     3901 gcggcacgag ctctgtatct ccatccgcaa caaactccct gctgtccgtt ctgcttttta
     3961 cgcgataaac aacatctccc ttctttagaa agtagccaga aagagtgcgc gccatatcca
     4021 cgtgaccgcc cgtgttgttg cgaagatcta tgatgattcc ttttacattc tgccgggcga
     4081 aatcttgaac ggctttctta aatttggaaa tagaacccgc atcttctcca aaaaatgcct
     4141 gcagccttac gtagcccacg cgcgaatctg cgagaaatgc ggtgcgcaca tattccagct
     4201 ggaatactcc gcgttccact ctgacaggaa atgagcttcc gcctctctga attccaagct
     4261 ccgctatgga ccctggctct ccgctgatca attcagatag tttagaaaca ccaacatatc
     4321 tggttggttg ccctccaatg ctgatgatgc ggtctcctgt catgagtcct gctttttcgg
     4381 caggaccacc gcgcattggt gcaacaatca aaggatatcc accctgaatt gtaatttcaa
     4441 ctccgattcc gaccaggtct ccaccttcag aatgattgaa gtccttctgc tcttcgcggt
     4501 ttagaaaacg agaataagga tcccctgtgg cttcgagcat tcccctgact gcgccttcga
     4561 aaagtttggc gcgcgataca gaatccacat gatagttttc aatgagatag agcacctgct
     4621 gcagcacgtt ttgatacatg tgcgacgctt cgatttgggc ttgagaagtt ttttcccctc
     4681 gcgatggagg ggcgttctgc gcgatcgccg gtgcgatcgc catgcaaagg atgagggccg
     4741 caactttcaa attcatggct tatccccggc aagagttttg tacagctctg gattgcgcgt
     4801 ccttacaatg tcttttacca ggtcgcattt cactctcttg ctcctgcagg caagttcgaa
     4861 gatctctttg tccgtcttct gggacaattc aggattcaga ggagcctgaa atcggagggt
     4921 catgaactcc tgaatcacac ggtccgcgtc tgcttccgtt ggcttcgcat aacaaccgct
     4981 aagcgccgcc aaaagtaaaa ccatcaggat ttttttcatc cttcctccct cgcaccaacg
     5041 acggcaccga gctccgttat ttgctgcatt gcgcgcagcg tcacactttt ctcttttgct
     5101 ccgcccttgc cgacttcttt tgcagccgcc tccggcagaa caatggtctt gtatcccagg
     5161 gaagaaagct cccgaatccg cgcggacacg gcaccggggc tgcgaacttc tccggtcaaa
     5221 ccaacttctc caatgaatgc aa
//
LOCUS       DAOQJL010000242         5239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9183_length_5239_cov_4.673418, whole genome shotgun sequence.
ACCESSION   DAOQJL010000242 DAOQJL010000000
VERSION     DAOQJL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_5239_cov_4.673418"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /locus_tag="PLG78_11190"
     CDS             complement(<1..2103)
                     /locus_tag="PLG78_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23726.1"
                     /translation="MRYYVVLMFLLISSALHAVEPDVFVGGKKILFAELKASEWKTLS
                     LRDRRMMAPRLVDYGTFNATRRSEFVELLSAESDPYLRAHAIVARAFFRSADDRIVRD
                     ELQSQLSRADSISRPILEYYAAMLAKMPAANLSSLACKFSELDDSCRAIKLRVYIEQL
                     ALLPQVSVQQIRDLQSMAAPLLLKNPIKPAMYHRIAEGVPEKLSRLRLPLEAAIIQER
                     MLRGIESAWANDLRARIPFYLATAGDFASALRYTENRASLRNIDLRMARLDWMILSGR
                     YKQAVDSLTEIIEGNIDLSGLHGKDPWTGFTYSKEGLRLRLAMVLHLAGDSGRAARAL
                     EMLKDSKGKTDLGEPVALYSRIRLAQILLKDRPELAHKLAEDVTYEAQAQNWSQLEYY
                     ATILDGWALYYSKQYFPAVVNFTKAGGIVRDPPAEYSRLLGLMVSQMAMAPASPQIYS
                     IQRLKTMLASRPYHRAIYTIRDWVPEDAGQDFFIEQSVLNFETRKDRWSSLLLLEEFK
                     RLEEFYFHAGNNPGGARGLLTSDVWFRTLREFPSVRILSRGVLEDNADSLRASIQGLI
                     QPQALFLADDSAYLFPFQVRDKVYTYLVAPAVPRSRAPRLILREDMSVADYFALADSC
                     RRGPCKESFPELGKFAASFRILQVQWRTGDPDFQKLFRRQDLSVYLFYDGFLPPKQYK
                     STYHGAYKPACSTPGAPLD"
     gene            2183..2920
                     /locus_tag="PLG78_11195"
     CDS             2183..2920
                     /locus_tag="PLG78_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23727.1"
                     /translation="MKQMYLVFAFLISAAVSAQATQPGTPASTTPAASQEDPHNADNY
                     LEKQTALKGIDQARSEQQKDIRKLSVIVANYGSAVAGSDAELKKIKDLYEESSVLYYR
                     QQYVKSREILNQAKTQTSELYKKFDDMYAKQTDELMIQCAKGVANLETTQTPGGGNSP
                     EWSTVLILNQSRLRTAFGHVVIAQDLNREKRYDDAIDHYRLAKLFGIGLLRGLEEDPQ
                     KAKAIDDKYKIDLQDATGGRAASAPSK"
     gene            2962..4305
                     /locus_tag="PLG78_11200"
     CDS             2962..4305
                     /locus_tag="PLG78_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNE23728.1"
                     /translation="MHRPIVFFLASFFAGCLPLFAFPDEIPVVMDPGHGGAIVPRKDD
                     KWDPVTRQYLDFYLSGMMYNQYTEQEIVLDLSKKVRKYLELTRSDDGWLEFEKILRLF
                     SDQKDFERVRFKIDMAREDGWQNRGKPVTDPEVNAPYRMYDYPNPARDMRMEMGRVSY
                     INSKQPYLVLALHLNPAEGTNPGGMGAVLSPGYPTFDLLRRITLGQAPADAFYKLPWA
                     DKWLISDPGFTQFESAKGDAWVYFHGYRMLKNNTPWLGKNRGIRWNMITWKYSDPPGW
                     EKLAAKAGPGPYAIKYAEWKPEGKFWDRERSPMELWRHEGGPLGYGGDNHYASDELLR
                     FVQMGVPIQVPERRAPGSMGEIVPPFVSTYGLPTFSNAIIAYLEIGHLNRDRDRTMVL
                     QNSEELARSLAAGIYSLFRGLSVKKTGKVRPKGAPLNFAKYENAPGGNYFRAVTE"
     gene            4305..5126
                     /locus_tag="PLG78_11205"
     CDS             4305..5126
                     /locus_tag="PLG78_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23729.1"
                     /translation="MRWQSAEVTAAAPSPWKDLPESPISFLVEVNGSNIVMVPGINDA
                     PACILTVEEVRLPSTWTLIRRITSIPATVVARMVQPFLPVENTNQTLCDILGWKRDYQ
                     NLTEAADLLSFDSQTIQLSCMHDLPVPVLRLFARIQEKTQLLQIFKTRSVRKNLVREI
                     ILDLYDLEEGARTRVINELLRSAQSTDSLAGEDLRDLVRKERHPKSEQIRQQIKEIKK
                     SMNLPSGLQIEVPADLENGQTKITLSFRRQEEFQKLLGEMDQAFSAKLSEILKLV"
BASE COUNT         1402 a         1345 c         1299 g         1193 t
ORIGIN      
        1 gtctagcggc gcaccaggag tggaacaggc aggcttgtac gctccgtgat aggtactctt
       61 gtattgcttt ggcggtaaaa acccgtcgta gaagagatac acagaaagat cctgtctgcg
      121 aaacagtttc tggaaatcag gatccccggt tctccactgc acctgcagaa tccgaaaaga
      181 ggccgcaaat ttgccgagct ctggaaacga ttctttgcag ggccctcttc tgcatgaatc
      241 agcaagcgcg aaatagtctg ctacgctcat gtcctcgcgc aagatgagtc tgggtgctct
      301 ggaccttgga actgcaggtg ctaccagata ggtatacact ttgtctcgaa cttgaaaggg
      361 aaacagataa gccgagtcat ccgccagaaa gagtgcctgg ggttgaatga gtccctgaat
      421 gctcgcgcga aggctgtctg cattgtcttc tagaacacca cgcgacagga tccgaacaga
      481 gggaaattca cgcaacgtgc gaaaccatac atcggatgta agaaggccgc gcgctccgcc
      541 tggattgttc cccgcgtgaa agtaaaactc ttccaacctc ttaaactctt ctaacaatag
      601 aagagaagac catcgatcct ttctcgtttc gaaattcaaa acagactgct ctatgaagaa
      661 atcctgtccc gcatcttctg gcacccagtc tctgatggta tagatggccc ggtgatacgg
      721 acgagaagcg agcatcgtct tgagacgctg gatagaatag atctgaggcg aagcgggcgc
      781 catggccatc tgcgaaacca ttagccccag cagtcgcgaa tactctgcgg gcggatcgcg
      841 cacgattcca cctgcctttg taaaatttac gaccgcagga aagtattgct tactataata
      901 gagtgcccag ccgtccagaa tcgtagcgta gtattctagc tgagaccagt tctgtgcctg
      961 cgcttcgtag gtaacgtctt ctgccagctt atgagcaagc tcgggcctat ctttcaacag
     1021 aatttgcgcg agccgaatgc gcgaataaag tgcaacaggc tccccgagat ccgtcttccc
     1081 tttggagtct ttgagcattt ccagggcgcg ggcggcgcgt ccgctgtctc cagccagatg
     1141 aagcaccatg gccaatcgca aacgcaatcc ttctttggaa tatgtgaagc cagtccacgg
     1201 atccttcccg tgtagaccgc tcaaatcaat gtttccttct atgatttcag tgaggctatc
     1261 cactgcctgt ttgtatctac cggatagaat catccagtcc agtctggcca tgcgaaggtc
     1321 aatgttgcga agtgacgcgc gattctccgt gtaccgaaga gcggacgcga aatcgccagc
     1381 agtggccaga taaaaaggaa tgcgagcgcg caaatcattc gcccatgcac tttcaattcc
     1441 gcgaagcatt ctctcctgga tgatggcggc ttccagtgga aggcgaagcc tgctgagttt
     1501 ttctggaacg ccctccgcaa tgcgatggta catggcgggc ttgattggat tctttagcag
     1561 aagaggcgcg gccatcgact gcagatcacg aatctgttgc acactgacct gaggcaacaa
     1621 tgcaagctgc tcgatgtaga cgcgaagctt gatcgctcta caactgtcat ccagttccga
     1681 aaacttacat gcgagagaag agagattggc agcaggcatt tttgcaagca tggctgcata
     1741 gtattccaga atcggtcttg agatggaatc tgctctggat agttgtgact gcaactcatc
     1801 tcgcacgatc cgatcatctg cgcttctaaa gaaagcacgt gcgacgattg catgggcacg
     1861 gagatatgga tccgattcag cggaaagaag ttcaacaaat tcgcttctgc gggtcgcatt
     1921 gaatgttcca tagtcaacga gacgaggcgc catcatcctg cgatcacgaa gagaaagagt
     1981 tttccactcc gatgccttca gctcagcaaa taagatcttt ttgccgccga caaacacatc
     2041 cggctccaca gcatgaagag cactcgaaat aagaaggaac atcaagacga cataatatcg
     2101 cacaggacag aatcagctca cgagtccgaa ctgtaaaacg tttttggttt gacgaggaaa
     2161 caagcgggca catcacaaca gcatgaaaca aatgtatctg gtcttcgcat ttctcatctc
     2221 agccgcagta tccgctcaag ctacgcagcc gggaacgccc gcatccacta ctccagcggc
     2281 gtcacaggaa gaccctcata acgcagacaa ttacctcgaa aagcaaacgg ccctcaaagg
     2341 cattgatcag gcaagatcag agcagcagaa ggatattcgc aagctttctg tgatcgttgc
     2401 aaactacggt tcagcggtgg caggctcaga tgcagaacta aagaaaatta aggatctcta
     2461 tgaagagtca tccgttctct actaccgcca gcagtatgta aagtcccgcg aaatcttaaa
     2521 ccaggcaaaa acacagacat ctgaactcta caagaagttc gatgacatgt atgcaaaaca
     2581 aacggacgaa ctcatgattc agtgcgccaa aggagtagcc aatctcgaaa caactcaaac
     2641 tccgggtggc ggaaactctc ctgaatggtc cacagtgttg attctcaatc aatctcgtct
     2701 gcgcactgct tttggtcatg tagtcattgc ccaggacctc aatcgtgaaa aacgctatga
     2761 tgatgcaatc gatcactaca gacttgcgaa attgtttgga attggtttgc ttcgcggact
     2821 ggaagaagat ccccagaaag caaaagcaat cgacgacaaa tacaagatag atttgcagga
     2881 cgcaactggc ggcagagctg cctcagctcc ttctaagtaa aatcccttga cggcgcgccc
     2941 cgcgccgtct gtgtcaactc agtgcatcgc cccatcgtct tctttctcgc atccttcttt
     3001 gcaggatgcc ttccactctt cgctttcccg gatgaaattc cagtcgtcat ggaccctggg
     3061 cacggcggtg ccattgtccc gcgcaaggat gacaaatggg accctgttac gcgtcagtat
     3121 ctggacttct atctttccgg gatgatgtac aatcagtata cggaacaaga aatcgttctg
     3181 gatctttcaa aaaaagtccg caagtacctg gaactcacgc gatcggacga tggctggctt
     3241 gagtttgaaa aaatcttacg actgttttcg gatcagaaag atttcgagcg cgtccgattc
     3301 aaaatcgaca tggcgcgcga agatggatgg cagaaccgcg gaaagcccgt aacagatcct
     3361 gaagtgaacg ctccgtaccg aatgtatgac tacccgaacc cggcgcgtga tatgcgcatg
     3421 gagatgggcc gagtatccta tatcaacagc aagcagccgt acctcgttct tgctcttcat
     3481 ctaaatccgg ctgaaggaac caatccaggt ggaatgggtg ctgttctttc tcccgggtat
     3541 ccaacgtttg atctattgcg cagaattaca ctggggcaag cgccggcaga tgcattttac
     3601 aaacttccct gggcagacaa atggctgatc agcgatccag gtttcactca gtttgaatca
     3661 gcaaagggag atgcgtgggt ctactttcac gggtatagaa tgctcaagaa caataccccg
     3721 tggctgggca agaatcgcgg aattcgctgg aacatgatta cgtggaagta ttcagatcct
     3781 ccgggctggg agaagctcgc agctaaagcg ggaccaggcc catatgctat caaatacgca
     3841 gaatggaaac cagaaggaaa gttctgggat cgcgaaagaa gcccgatgga actgtggaga
     3901 catgaaggcg gccctcttgg atacggagga gacaatcact acgctagcga tgaattgctt
     3961 cgattcgttc aaatgggtgt gcccatccag gttccagaaa gacgcgcccc ggggtctatg
     4021 ggagagattg ttccgccttt tgtgagcaca tatggactgc ctactttttc caatgcaatc
     4081 atcgcttatc ttgaaatcgg gcatctcaac cgggacagag acagaaccat ggtgcttcaa
     4141 aattcggaag agctggcacg aagcctggcg gcgggcatct actctctctt tcgcggccta
     4201 tctgtcaaaa aaacaggcaa ggtaaggcct aagggcgctc ctctgaattt tgcaaagtat
     4261 gaaaacgctc cgggcggaaa ttactttcgc gcagtcacag aataatgcgc tggcaatcag
     4321 ctgaggttac cgcggctgca ccctctccgt ggaaagatct acctgaatct cctatctctt
     4381 tcctggtgga agtaaacggt tccaatattg taatggtccc aggaataaac gacgcacccg
     4441 cttgcattct gacagtcgag gaagtcagac tcccgtccac ctggacgctg attcgtcgaa
     4501 taacgtctat cccagcgacc gtcgttgcca gaatggttca gccatttctg cccgttgaga
     4561 atacaaatca aacgttatgc gacattcttg gctggaaaag agattatcag aacctgacag
     4621 aagccgcgga cttgctctca tttgattctc aaactattca gctatcctgc atgcacgatc
     4681 tgcctgtgcc tgtgctcaga ctctttgcca gaatccagga aaaaactcaa ttgcttcaaa
     4741 tattcaaaac tcgcagtgta cgaaagaacc ttgtgcgcga aatcattctg gatctgtacg
     4801 acctggaaga gggcgcccgc acccgtgtga taaacgaatt actccggtcc gcacagagca
     4861 cagattcact ggctggagaa gatctccggg atctggtgcg caaggaacgc catccaaaat
     4921 cagaacaaat cagacagcag atcaaggaaa tcaagaaatc gatgaatctg ccttccggcc
     4981 ttcagataga ggtcccggcg gatcttgaaa acggccagac aaagataacg ctatcatttc
     5041 gccgccagga agaatttcaa aaactcctgg gagagatgga ccaggcattc agcgcgaaac
     5101 tctctgaaat cctaaagctg gtttaggacg agcggcgatg cgccgcgaag accgtcagcg
     5161 tttcttagcc ttcttcgctg ctttcttcgc gggtttagat tttgcggccg gttttttcgc
     5221 agtctttttg gcacttttc
//
LOCUS       DAOQJL010000243         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9222_length_5227_cov_4.313418, whole genome shotgun sequence.
ACCESSION   DAOQJL010000243 DAOQJL010000000
VERSION     DAOQJL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_5227_cov_4.313418"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLG78_11210"
     CDS             complement(<1..474)
                     /locus_tag="PLG78_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23730.1"
                     /translation="MPSVLDLVGAFYRLGTRYLPVVDPVKAEVIGVLSRDHITAISAD
                     PERARREFETIPSEMYLEQPLSPAVMQDVGAHHTVFVLFPDLTESKQEASGVLAMLSH
                     YRKKMPVTAHAKDDTDASHPEDVKLWLSRILLASVPLPLFAIDLHPFAEGIKAGAD"
     gene            complement(496..1494)
                     /locus_tag="PLG78_11215"
     CDS             complement(496..1494)
                     /locus_tag="PLG78_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23731.1"
                     /translation="MMQIYVNNTELEAQLTDEQNLLQVYDAVSDWTRANHRYILGIQV
                     DQKIMAPDHLEEINTASVDRVDFRIGDEIEMMAESVAEMDRYIDRIGNSLFDRTHLTA
                     EEIQNLKDGVRWISEIIASSCAIMHLNSDEIMCPVVGEDGAETKTVHELLAALATSAE
                     ELKTDGPDSVLVFLSILRDLRFFVIKLKMQLLALNATEAELVSSLEEFESRIPVLMEE
                     LVQVTTQFQSGQDFQALESLDAVVEKLNTYLSAMLAVEYRLARDGSLPAQKGGEGMTF
                     QQVSEDLTGILKDISSAMVDSDIVAVGDILEYELTARLSQMPPFLKQLREILVARS"
     gene            complement(1491..2948)
                     /gene="fliD"
                     /locus_tag="PLG78_11220"
     CDS             complement(1491..2948)
                     /gene="fliD"
                     /locus_tag="PLG78_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNE23732.1"
                     /translation="MPSPPMPGLGTGADTQKVIQQLIEVERRPLKRIILDNESSGQSI
                     KIWEEARRRSRNLQDKSQVLYSFSGAFATKAIVSSDPGAVTGQASPDMDDQSMQIEVS
                     KLATHHRIHSAPVSAEKDLPAGTFTIKVGEKSLEYDFPGGKLTALVRLIKSKAAGTID
                     VNMIRKDSETSIVVLRSLTSGTKGKMEFVDAGVLATLGLPPGAGGASEVEAPQDAVLK
                     LHGVEVTRTSNENITDVLEGASLTLRKVTTGPVTLTVQANTSVIEKQIADWITAYNEL
                     MQFLRENSKTVSREEFERNRPEGDDVGEGLRRLKASSGLFTTDSNVRQLMFGLQGIVA
                     ASYPNSRPNGVRVLADIGISSTAISRGEADPAAGLLMLDADKLRSALSQDAAAVKDIF
                     ASDTNEDSVIDNGAAFRMQQMLAPYTRMSGGLITTRIDLIKSQISDNKEKIRRKEMEL
                     VGKEAELRRRFGRMDSAIRESKNMSQSLGKLGEGQ"
     gene            complement(3038..3361)
                     /locus_tag="PLG78_11225"
     CDS             complement(3038..3361)
                     /locus_tag="PLG78_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23733.1"
                     /translation="MSFLDLILQLVVYTASGIAASYYFYNWKRKDLLGGFWGGTVIGL
                     VGAVLISWVTSRDAWFIRLVSWLMIPKFGDGFQIPVNLIASVLGGLLFVAILNRINHN
                     KDRRF"
     gene            3474..4367
                     /locus_tag="PLG78_11230"
     CDS             3474..4367
                     /locus_tag="PLG78_11230"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNE23734.1"
                     /translation="MPEHPVAASGKTRVYGILGNPVAHSLSPLIHNAAFQFKKIDAAY
                     VPFHTTQTGAFLKKAVVSMGIRGLSITIPHKIWAAKAADQKDPLTKLCGAANTWIIEN
                     GIVSAHNTDGPGAVRAMQEKHKGVGKRYLILGYGGTALAIAHALLLESSPRSITITGR
                     NLKKAARFAEDLRQAHARHRSLIDSAPLSQIAPEQIDAVIQTTPSGMNAEKGSKVLPE
                     LGFDPDWITKNHLVFDVIYVPMRTPLIELAERRGSTIVYGYKMLLYQAVRQFELFTGQ
                     SAPESLMERLLLSHLRNGTRR"
     gene            4364..>5227
                     /locus_tag="PLG78_11235"
     CDS             4364..>5227
                     /locus_tag="PLG78_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNE23735.1"
                     /translation="MKLERELAIRINPEKSRRFTAEQFAANLARLTDAALSMQGEDRT
                     IVSVVGTNGKGSTSYYLAQLFESQTGLYTSPHLHSVYERIRVNLESISETECDAALLR
                     LEKQHDLSNYSYFELLTILAGFLFHEKNIPVQIFEAGLGGRLDATRIFRAQYVVLTSI
                     GLDHTELLGNSHEEILAEKLGICTKNARFVLCANQPHLSRKTVEEMSPVPVLFFDEPY
                     LPNSYLAFNQRFAIFCAKKIAEDSSRTLSSISSPLRDPPGRLERRNLNQRAVFFDNAH
                     NPDAIHTALSSL"
BASE COUNT         1317 a         1365 c         1273 g         1272 t
ORIGIN      
        1 atccgcgcca gctttgatgc cttccgcaaa ggggtgcaga tcaatggcaa acagaggaag
       61 cggaacagaa gcgagcaaga ttctagagag ccagagcttc acgtcttctg gatgagaagc
      121 gtctgtgtcg tcttttgcat gcgcggtgac cggcatcttc tttctatagt gactgagcat
      181 ggcaagcacg cccgatgctt cttgtttgct ctccgtaaga tctggaaaaa gaacaaacac
      241 agtgtgatgt gcacctacat cctgcatgac tgcaggcgaa agaggttgct ccagatacat
      301 ctcagaagga atggtctcga attcgcggcg cgccctttca ggatctgcag aaatagcagt
      361 gatgtgatcg cgcgagagca cgccaatgac ttccgccttc actggatcca ctaccgggag
      421 gtaacgagtg cctaaccgat aaaaagcgcc gacgaggtcg agtacactgg gcactctgcc
      481 tctgtgacgc gcgcgtcagg agcgtgcaac caggatttcg cgaagctgct taagaaaggg
      541 cggcatttga gaaagacgag cggtcagctc atattccagg atgtctccta cagccacgat
      601 gtccgaatcc accatggcgc tggagatgtc tttcaggatg ccggtcagat cctcggaaac
      661 ctgctggaag gtcattcctt ctccaccttt ttgagcgggg agcgagccgt cgcgcgccag
      721 acgatactct actgcgagca tggcagaaag atacgtgttg agcttttcta cgactgcatc
      781 gaggctttcc agagcctgga aatcctggcc cgattgaaac tgagtggtca cttgaacgag
      841 ttcttccatg agcacgggga ttcttgattc gaactcttcg agagaagaga cgagctctgc
      901 ttccgttgca ttgagcgcaa gaagttgcat ctttagtttg atcacaaaga atctcaggtc
      961 acgcaggata gagagaaata ctagaaccga atccgggccg tctgtcttca gctcttctgc
     1021 actggtcgca agagcagcga gaagttcgtg aacggtcttt gtctctgccc catcttcgcc
     1081 cacaaccgga cacatgattt cgtccgaatt cagatgcatg atggcgcaag aagaagcaat
     1141 gatttcagaa atccaacgaa ccccgtcttt aagattctga atttcttctg cggttaaatg
     1201 agtgcgatca aacagagaat tcccgattcg atcaatgtag cgatccatct ccgctacact
     1261 ctccgccatc atttcaattt cgtcaccgat tcggaagtcc actcgatcca cagaagcagt
     1321 gttgatttct tccaggtgat ccggcgccat gatcttctga tccacctgga ttcctagaat
     1381 gtagcgatga ttggctctgg tccagtcact gactgcatcg tatacctgga gcaaattttg
     1441 ctcatctgta agctgagctt cgagttcagt gttgtttacg taaatctgca tcattgcccc
     1501 tcaccgagtt tgcccagaga ctggctcatg tttttgcttt cgcggatcgc gctatccatg
     1561 cgaccaaacc tgcgcctgag ctctgcttct ttgccaacca gttccatttc ctttctacgg
     1621 atcttttctt tattatcgga gatctgagat tttatcagat caattctagt agtgatgagc
     1681 ccgcccgaca ttctggtgta gggcgcaagc atctgctgca tgcgaaatgc tgctccgttg
     1741 tctatcacgg aatcctcatt tgtatcggac gcgaagatgt ctttgactgc ggcggcgtcc
     1801 tgcgaaagag cagagcgcaa tttgtcagca tctagcatca aaagaccggc tgccggatcc
     1861 gcctctcctc tggaaattgc agtagaacta attcctatgt ctgcaagaac ccgcactcca
     1921 tttggtctgc tgtttggata gctggcagct acaatacctt gcaatccgaa catgagctgg
     1981 cgtacgtttg agtctgtagt aaagagtcca gagcttgctt tgagacgacg aagaccttct
     2041 ccgacatcgt ccccctcagg acgattgcgt tcgaattctt cgcgactcac tgtctttgaa
     2101 ttctcgcgca agaattgcat cagttcatta taagcggtga tccagtctgc gatttgcttt
     2161 tcaatcacag acgtatttgc ctgcacggtc agcgtaactg gcccggtagt cactttgcgc
     2221 agtgtgagac ttgcaccttc caggacatct gtaatgttct cgttagaagt cctggtcact
     2281 tctactccgt gcaacttcaa cactgcatcc tgaggagctt ccacttcaga ggcaccaccc
     2341 gctccagggg gaagacccag agtcgcaagc acgcctgcat ctacgaattc cattttccct
     2401 ttggttccgc tcgtcagact gcgcaaaacc acgatggaag tttcgctgtc cttgcgaatc
     2461 atgttcacgt ctatcgttcc agcggctttg ctcttgatga gccgcacaag agcggtgagc
     2521 ttgccaccgg ggaaatcgta ctcaagactc ttctccccga ctttgatagt gaaggtaccc
     2581 gcaggcagat ctttctctgc gctgacaggc gcggaatgaa tgcgatgatg agtggcaagc
     2641 ttgcttactt ctatctgcat ggattgatca tccatgtctg gcgaagcctg ccctgtgact
     2701 gcacctggat cgctcgatac aatcgccttg gttgcgaatg cgccagagaa gctatagagc
     2761 acctgcgatt tatcctgaag gtttctggaa cgtctcctgg cttcttccca gatcttgatg
     2821 gactggcctg aggattcgtt gtcgagaatg atgcgcttga gcggtctgcg ttctacttca
     2881 atgagctgct ggattacttt ttgagtatcc gctcctgttc cgagtcctgg catgggaggc
     2941 gatggcataa ttggtacctg atactatcat cgggcgtttt gaaaaaatga taatagccag
     3001 aaagcgcaaa gatagcccgc acgcagggat gcgagagcta gaaacgacga tctttgttgt
     3061 gattgattct gttgagaatc gccacaaaga gaagaccgcc cagaacggag gcgatgaggt
     3121 taaccggaat ctgaaatcca tccccgaatt tgggaatcat aagccagctc accagacgaa
     3181 tgaaccacgc atcccggctg gtcacccagc tgatgagaac ggcacccacg agtccaatga
     3241 cagttccacc ccagaagccg cccagaaggt ctttgcgttt ccaattatag aaatagtaag
     3301 acgcagcgat cccagatgcg gtgtagacta caagttggag tatcagatct agaaaggaca
     3361 tgttgtaaca ataaacagat cggctatttt gccggctgag cttaccatga tttatccatg
     3421 cctgcggggg aaatagctct ttacaacaga gcgggcggct ccactcaaaa aaaatgcctg
     3481 aacacccagt agcggccagc ggcaaaacca gggtctacgg aatcctgggc aatcctgtcg
     3541 cgcattctct cagcccgctc attcacaacg cagcctttca attcaagaag atcgatgcag
     3601 catacgtgcc cttccacaca acgcaaacag gagcatttct caagaaggca gtggtttcga
     3661 tgggaattcg ggggctgtcc attaccatcc cgcacaaaat ctgggctgcg aaagcggcgg
     3721 accagaaaga tcctcttacg aagttgtgcg gggcggccaa tacctggatc atcgaaaatg
     3781 gaattgtaag cgcgcacaat acagatggac ctggtgcggt tcgggccatg caagaaaaac
     3841 acaaaggcgt gggcaaaagg tatctcatcc tcggttacgg cggcaccgca cttgcgatcg
     3901 cccatgctct gctcctcgaa tcttctccgc gctccatcac gatcaccgga agaaatctaa
     3961 aaaaggcggc acgcttcgca gaagacttaa gacaagcgca cgcgcgtcat cgctccttga
     4021 tcgattctgc accactctca cagatcgctc ctgaacaaat cgacgctgtg atccagacga
     4081 ctccgtcggg aatgaatgcg gaaaaaggaa gtaaggttct tccggaactc ggttttgatc
     4141 cggactggat tactaaaaat catttagtat ttgatgtcat ctatgttccc atgcgcacac
     4201 cattgatcga actggcagaa agaagaggat ccacaattgt ttatggatac aaaatgctac
     4261 tctaccaggc tgttagacag tttgaactgt tcacgggaca atctgcgccc gagtcattga
     4321 tggaacgact gctcctctct caccttcgaa acgggactcg cagatgaagc tcgaacgcga
     4381 gctcgccatc cgaatcaatc cagaaaaaag caggcgattc acagcagaac agtttgcagc
     4441 aaaccttgca cggttgacag acgccgctct atccatgcag ggcgaagata gaacgattgt
     4501 gtctgtggta ggaacgaacg ggaaaggatc cacttcctat tatctggcgc aactgtttga
     4561 gagccagacg ggtctttata ccagccctca ccttcattct gtttacgaaa gaattcgagt
     4621 caacctggag agcatatcag aaacagaatg cgatgcggca ctattgcgtc tcgaaaagca
     4681 gcacgatctt tccaattaca gctacttcga attgcttacg attctcgcag gcttcctctt
     4741 ccacgaaaag aacattccgg ttcagatttt tgaagcgggt ctgggcggta gactcgatgc
     4801 gaccaggatc tttcgcgcac aatacgtagt acttacttcc ataggcctgg atcatacaga
     4861 attgcttggc aattcacacg aagaaatact cgcagaaaaa ctgggaatct gcacgaaaaa
     4921 cgcgaggttc gttctgtgcg ccaatcagcc tcatctttcg agaaagaccg tggaagaaat
     4981 gtctcctgtt cccgttcttt tcttcgatga accgtatctt ccaaattctt acctcgcatt
     5041 caatcaaagg tttgcgatat tctgtgcaaa aaaaatagcg gaagattcca gccgtaccct
     5101 gtcgagcatc tcatcgcctc tgcgtgatcc cccgggcaga ctcgagagaa ggaatctaaa
     5161 ccagagagca gttttctttg acaatgcaca caacccggat gcaatccata ccgctctatc
     5221 ttctctg
//
LOCUS       DAOQJL010000244         5226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9224_length_5226_cov_4.428350, whole genome shotgun sequence.
ACCESSION   DAOQJL010000244 DAOQJL010000000
VERSION     DAOQJL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9224_length_5226_cov_4.428350"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1405
                     /locus_tag="PLG78_11240"
     CDS             155..1405
                     /locus_tag="PLG78_11240"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE23736.1"
                     /translation="MNLIGRMRKICGLLILLLSSCARQFDDNTDFLTRSIVAYDVFRL
                     IDIPRFAYAPLSGGNIGGFSVNRVTGQITLVGTYSTGISPSALVTDTQGKFAYATGGT
                     TLYAASIDQTTGALVSAGTQATGAAPSAPRMDPYNRFVYLISGAGSVTGYAINQTSGA
                     TTLIGSWPSAVGYGKPLVHTSGRYLYANTGSGIYMYSIDQTSGALTSLGSISDGGIAG
                     QMELDSTGKYLYHTYPGSVTVFSVNSDGTLSNIQTVAGLAAPSSLNLEHPFKRFAYLT
                     SLTGGTTVMMYQVSNGALSLLGSVAGGTNTGAIRIEPSGRFAYLCNQGSGDVTQYSID
                     QFTGLLYSFGSVVSGSGPIIASPDPTGRFLYSPNATGASINQYSIDPFSGQLRLIQNT
                     PTSGNPSYVGLVSYPASAIYQYYY"
     gene            complement(1411..4323)
                     /gene="polA"
                     /locus_tag="PLG78_11245"
     CDS             complement(1411..4323)
                     /gene="polA"
                     /locus_tag="PLG78_11245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNE23737.1"
                     /translation="MEKRKTILVIDAHAMAYRAHFALERQNLTNAAGQNTGAIFGFFR
                     MFFKILQDFTPEETLVAWDPPGGGFRGDMYKEYKATRKPMPDDLRSQISEIKEIVRNM
                     GFATCEVPGFEADDVMGSMATRFGKASDVLLLTSDKDCFQLITKNVSMLKGAKGVTEF
                     VRVDPAWVENEIGVRPDQIPEYMALVGDTSDNIPGAKGIGPKTAAELIQQYKSIDGIY
                     KNLPRIAKESVRKKLQESESDVRLSRDLATIRTDIKEVLDFPEAKLRTPDYLREDVVQ
                     FFRQEGYRAIAEDLRRAAEKRRGKAKVAAASVAQEGEDVSSSSAQKKSAGKKDAASST
                     KPKQKSKKGALDQGTLFEEEPEQRMFSGKEHFLGTDVEYEMVTDAEQLKRALREHNRS
                     VVVVDTETDSADPMRARLVGISISAREKKATYIPLPPPGSPYESAGLALNEVRSLLKE
                     FFGEKTRSFVGQNIKYDYTVLRRHGMPLNAVAFDTMLAGYLLNPNIRRNSLDDLALDY
                     LDYSTIRYEDVVGTGRKQVTMDQLPPANIRDYACEDADLTLRLHQVLGRKLKENKLLE
                     INDSLEVPLIPVLADMEVAGMAIDLQYFKELSRDYKQKITKLEKKIFEEAGQVFNVNS
                     TRELQFVLIEKLKLPMAKKIKTGFSTDQKELDRMRGMHPMIDHLLEYRKYTKLLGTYI
                     DALPELVHPETGKLHTNFHQAVAATGRLSSMDPNLQNIPVREDTGKAIRRGFVCSKGR
                     VLLSLDYSQVELRIMAHYSHDPALMEAFTRDDVDIHARTASSIFGVAEDQVTADMRSK
                     AKVVNFSIIYGVTDFGLSQSLQVPRAEAKMYIEKFFEKYPGVKKYMDDTIAFAGKNGY
                     VETLFGRRRQIPDIHSPNRFRREGAERTAINTPIQGTSADLIKRAMIDIHAKMKSKKL
                     ESQMILQVHDELLFDVVPSEKDEVVSIAKGEMESTRLRVPLRVDHGFGQNWDEAKG"
     gene            4404..4730
                     /locus_tag="PLG78_11250"
     CDS             4404..4730
                     /locus_tag="PLG78_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE23738.1"
                     /translation="MLNTKKVGDKMVVYLEGRLDVSVANDVEEELNRIIETDNQKHLV
                     LNMEKVEYMSSSGFRACISTLRKLNAKDGTLKLCSIRPSVKRIFDVIELTSLFDICET
                     EADALK"
     gene            4732..>5226
                     /locus_tag="PLG78_11255"
     CDS             4732..>5226
                     /locus_tag="PLG78_11255"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNE23739.1"
                     /translation="MQGLLDRIVAAKREEIRSLKSIQPARVESHFLAAVKRAPGDSVR
                     VIAECKKTSPSSGRMRETYEPEKIATEYQRLGASALSVLTDRQFFEGDPAHVAMAKKS
                     GLPALRKDFVIDEKQIADSAVLGADAVLLIVRILEVSQLADYIAYADSLGLPALVETH
                     NEKEI"
BASE COUNT         1256 a         1381 c         1244 g         1345 t
ORIGIN      
        1 aagggcaatg cctacattta cgagatcacg tgcgatagtt tcgaactgag ctgattgagc
       61 catggacaat cttttcccct gtacggaggc tttcaagtca aacttgaagg ccgtttaggg
      121 ctggacaagc ccccgcgagg agccaaatca agttgtgaac ttgataggtc gcatgcgaaa
      181 gatatgcggg ttactgatac tactattgtc atcttgcgcc cgccagttcg atgacaacac
      241 ggacttcctc acgcggtcca ttgtcgccta cgacgtattc agactcatcg acataccacg
      301 cttcgcgtat gctcctctta gcggtggaaa cattggaggg ttctcagtaa accgcgtaac
      361 gggacagatc acacttgttg gaacctattc tacagggatc agtccttccg cactcgtaac
      421 agacacacag ggaaagttcg catatgcaac cggtggaaca actctctacg cggccagtat
      481 tgatcagacc acaggcgcgc ttgtaagcgc gggtacgcaa gcaaccggcg ctgcgccgag
      541 cgcaccgcga atggatcctt acaatcgctt cgtctacctc atcagtggcg ccggaagcgt
      601 aacaggctat gcaatcaatc agaccagcgg tgcgacgact ctgattggat cgtggccttc
      661 cgctgttggg tacggaaagc cactcgttca tacaagcggt cgatatcttt atgcgaacac
      721 tggctctggc atctatatgt attccattga tcagacttcg ggggcactca caagcctggg
      781 atccatcagc gacggcggga tcgctggaca aatggaactg gattcaactg gaaaatatct
      841 ctaccatacg tatccaggat ctgttactgt gttctccgtc aattcagatg gaacgctttc
      901 caacatccag acagtggctg gactggcggc gccttcttct ctcaatctgg aacacccctt
      961 caaacgattt gcctacctta caagccttac aggtggaacg acggtgatga tgtatcaggt
     1021 ttccaatggc gcactgagtt tacttggttc tgttgccggc ggcacgaaca ccggagccat
     1081 tcgcatcgag ccttccggaa ggtttgctta tctttgcaac cagggttcag gagatgtcac
     1141 acagtattcc attgatcagt ttactggttt gctgtactcg ttcgggagcg tcgtaagcgg
     1201 gagcggacca atcatcgcat cgcctgatcc aaccggacgg ttcttgtatt cgccaaatgc
     1261 aacgggagca agcatcaacc agtactcgat tgatccgttt tccgggcagc tacgcctcat
     1321 tcaaaataca cctacatctg gaaatccaag ttacgttgga ctcgtttcct atccggcttc
     1381 cgcaatttat caatactact actgaatcgc ctaacccttc gcttcgtccc agttttgtcc
     1441 aaatccatga tccacgcgaa ggggcacgcg aagccttgtg ctttccattt ccccttttgc
     1501 gatcgaaacg acttcatcct tttcagaggg cacaacatca aacagaagtt cgtcgtgcac
     1561 ttgcagaatc atctgagact caagtttctt tgatttcatc ttcgcgtgaa tatcaatcat
     1621 cgcacgcttg atcaggtctg cactggttcc ctggatcggc gtattgatgg ctgttctttc
     1681 cgctccctcc cgacggaatc ggttggggga atgtatgtct ggaatttgcc tgcggcgacc
     1741 gaataacgtc tccacatatc cgttctttcc tgcaaatgcg atggtatcat ccatgtactt
     1801 tttgacgccg gggtatttct caaaaaactt ctcgatgtac atttttgctt ctgctcgcgg
     1861 cacctgaaga ctctgcgaaa gaccaaaatc ggttacccca taaatgatcg agaagttcac
     1921 aacctttgct ttggatcgca tatctgctgt tacctgatct tctgcaactc caaagatgga
     1981 agaagctgtt cgcgcgtgaa tgtctacatc gtcgcgcgtg aaggcttcca tcaaagccgg
     2041 gtcatgcgaa taatgcgcca tgatgcgcag ctccacctga gaataatcga gagacaaaag
     2101 caccctgccc ttgctacaaa caaaacctcg gcgaatcgcc ttgcctgtat cctctcgcac
     2161 aggaatattc tgcagattgg gatccataga tgacagacga ccagttgcgg ccactgcctg
     2221 atgaaagttc gtatgaagct tgcctgtttc tggatgaaca agctcaggaa gagcgtcaat
     2281 gtatgtgccc agaagtttgg tgtactttct atattcaaga agatgatcga tcatcggatg
     2341 cattccgcgc atgcgatcga gttctttctg atccgtagaa aagccagtct tgatcttctt
     2401 tgccatgggt agcttcaatt tctcaatcaa gacaaattga agttcgcgag ttgagttcac
     2461 attgaagacc tgacccgcct cctcgaagat cttcttttcc agctttgtga tcttctgctt
     2521 atagtcgcgc gaaagttcct tgaagtactg caaatcgatg gccatgcccg ccacttccat
     2581 atccgccagt acgggaatca gaggaacttc gagcgaatca ttgatttcaa gaagtttgtt
     2641 ctccttgagc ttacgcccga gcacctgatg gaggcgaaga gtcaaatccg catcttcgca
     2701 cgcatagtct ctgatatttg ccggtggaag ctgatccatt gtcacttgtt ttctgcccgt
     2761 accaaccaca tcttcgtagc gaatcgtaga atagtctaga tagtccagcg ccaggtcatc
     2821 caggctgttt cttctgatat tcggattgag cagataaccc gcaagcatag tatcgaaagc
     2881 cacagcattg agaggcattc catgcctgcg cagtacagtg taatcgtact tgatattttg
     2941 ccccacaaag gagcgtgttt tttccccaaa gaattctttg aggagtgacc ggacttcatt
     3001 caatgcaagc cctgccgatt cgtatgggga accaggaggt ggaagtggaa tgtaggttgc
     3061 cttcttttcg cgggcagaaa ttgagattcc aaccagacgc gcacgcattg gatctgcgga
     3121 atctgtttcc gtatccacga ccacaacaga acgattgtgt tcgcggagcg ctcgcttcaa
     3181 ttgctctgca tctgtaacca tttcatattc tacatctgtc ccgagaaaat gctctttgcc
     3241 agaaaacatt ctttgctctg gctcttcttc gaagagagtt ccctgatcga gtgcgccctt
     3301 ctttgacttt tgctttggct ttgtggaaga ggctgcgtct ttttttcccg ccgatttttt
     3361 ctgagcagaa gaggaagaaa catcttctcc ctcctgggcg acagaggcgg cggccacttt
     3421 tgctttccct ctccttttct cagccgcccg gcgcagatct tctgcgatgg cgcgataccc
     3481 ttcctgtctg aaaaattgaa ctacatcttc gcgcagataa tccggcgtgc gaagctttgc
     3541 ctcaggaaaa tcgagcactt ccttgatgtc cgtgcgaatg gtggcaagat cgcgagaaag
     3601 acgaacgtct gattcactct cctgcaattt cttcctgact gactccttcg caatgcgcgg
     3661 gagattttta tagatgccat caatggattt gtactgctgg atgagttccg cggctgtttt
     3721 ggggccaatg ccctttgcgc ctggaatgtt gtcgctcgta tcaccgacga gcgccatgta
     3781 ttctggaatc tgatcggggc gcacgccgat ttcgttttcc acccatgcag gatccacgcg
     3841 cacgaattct gtaacgcctt ttgctccctt gagcatactg acgttctttg tgatcaattg
     3901 aaaacagtct ttgtcagagg taagaagcag aacgtcggac gccttgccga accgagtagc
     3961 catgcttccc atcacgtcgt ctgcttcaaa tccaggtacc tcgcaggttg caaaacccat
     4021 gttgcgaacg atctctttga tttcagaaat ctgactcctg agatcatccg gcataggctt
     4081 ccgcgttgcc ttgtactcct tgtacatgtc gccgcgaaat cccccacctg gcggatccca
     4141 ggctacgaga gtttcttccg gggtaaaatc ctgaaggatc ttaaagaaca tccgaaagaa
     4201 tccaaaaatc gcgcctgtat tctgaccggc agcgttggtt aaattctgac gctcgagcgc
     4261 gaaatgcgca cggtatgcca tcgcgtgagc gtcgattact agaatggtct tgcgtttttc
     4321 catcttttcc tggctatcag gatgcttgac agggcgggcc tggcaatgca aggtgccccg
     4381 agtactgttt agaggtgccc tgaatgctca atacaaaaaa agtcggggac aagatggttg
     4441 tctatctcga aggccgtctg gacgtttccg tcgccaacga cgtagaagaa gaactcaacc
     4501 gtatcattga aacggacaac cagaagcatc tggtcctgaa tatggaaaaa gtggagtaca
     4561 tgagctcctc cggattccgc gcatgcattt ctacattgcg caaattgaat gccaaagacg
     4621 gcactctgaa gctttgctcc atccgccctt ccgtaaaacg catctttgat gtcatcgaat
     4681 tgaccagcct ctttgacatt tgcgaaacgg aagcagacgc actgaagtaa cgtgcagggc
     4741 ctgctagacc gcatcgtggc ggccaagcga gaggaaatcc gctcactcaa atccatccag
     4801 ccagccagag tggagagcca tttcctggcc gcagtcaaac gtgctcccgg cgattccgtg
     4861 cgcgtcatcg cagagtgcaa aaaaaccagc ccctcctccg gtagaatgcg cgaaacctac
     4921 gaaccagaaa agatcgcaac cgaataccag agactgggtg catccgctct ctccgtactt
     4981 acggacagac aattttttga aggggatcca gcgcacgtgg ctatggcgaa gaagagcgga
     5041 cttcctgcgc taagaaaaga cttcgtcatt gatgaaaaac aaatcgcaga ctcagctgtg
     5101 cttggagcag atgcagtctt actgatcgtt cgcattttag aagtctcaca gcttgcagat
     5161 tacattgcat acgcagactc tcttggcttg cccgcgcttg tggagactca caatgaaaaa
     5221 gaaatc
//
LOCUS       DAOQJL010000245         5211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9272_length_5211_cov_4.480217, whole genome shotgun sequence.
ACCESSION   DAOQJL010000245 DAOQJL010000000
VERSION     DAOQJL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9272_length_5211_cov_4.480217"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="purB"
                     /locus_tag="PLG78_11260"
     CDS             complement(<1..888)
                     /gene="purB"
                     /locus_tag="PLG78_11260"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNE23740.1"
                     /translation="MIDRYSNPEISRIWELDNKYRIWLDVELAVTRALHKRGQVPDQD
                     WKEIQQKAGFDTAKILEIEEVVQHDVIAFLTNVKDHIGEAGRHLHYGMTSSDLGDTAL
                     SLQMVQAADILLKRVDELVDTARTLAIRHKNQVMIGRTHGIHGEPTTLGLKFALFYEE
                     MKRNRARLLRAREEVAVGKMSGAVGTFSANDPEIEEEVLADLGLKPDPISTQVISRDR
                     HAYFQSVLAVIAGSLDRMAQEVRLLQKTETREVEEPFRAGQKGSSAMPHKRNPVVCER
                     ICGLARVIQSNAMAGYRNMP"
     gene            complement(892..1461)
                     /locus_tag="PLG78_11265"
     CDS             complement(892..1461)
                     /locus_tag="PLG78_11265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNE23741.1"
                     /translation="MIEIVAGTNRPGSKTIQLAQIIRGLYDLAGAPSQILDIASLPVE
                     IYQPASYKTKPAAFAPFQERILACKGIHMVVPEYNGSFPGALKYFIDMLKFPESLEHR
                     PIAYTGLADGLWGGFRAVEQLQMVCNYRNAYGLPERVWFPHIGKKMQDNRITDEFLFG
                     LLRDQVERFIKFANHHEKEAVHSIPNKSA"
     gene            complement(1519..2865)
                     /locus_tag="PLG78_11270"
     CDS             complement(1519..2865)
                     /locus_tag="PLG78_11270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE23742.1"
                     /translation="MAAATTALVVICSGCQTPEIEEVPFGPENPERIAQIQGQLESFL
                     AYWRARGQFYGMTVGVVKADKLVYKHETGMTQDRKYALGSITKIFTATAVMKLVEEGA
                     VDLDAPLTDYMEVRIERPELSSRPVTIRHLLSHTSGMPDLRYFHPELIASPELPFRIP
                     PQIVPAGMHYRYSNTGFMILGEMVSRRSGKPMDQFMREEIFEPLEMRQTKTSQLTGAS
                     GVATTIEDLSHFAMMYLNHGKYGGKTILSEETVNRMLTQPVYYPPAEFQEYCGLGWRV
                     KRGPKGVVTFFHIGGADYIAAWLQLFPRYRMAILYLGDPPKYDDYTMGMLLALQAKLG
                     DLATAYAGADTPLYLFKATPPPPEVFETYPGTYINVLTGREAVVTAKNDVLFFSRAGT
                     YAYPLGTQTNHVFTGGFQDLSHDFVHEPGTNKLLGIAAYDGYYVRKPPEDTDTPQK"
     gene            2960..4342
                     /locus_tag="PLG78_11275"
     CDS             2960..4342
                     /locus_tag="PLG78_11275"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE23743.1"
                     /translation="MTFRHIRIAGFAFLGILFVVPGLRGPGFSHQVEGIPGVELIMQE
                     ASPSEWAKKRLRNIQDTLRDFVHSRAGRPALPNGLVIVNNVHEKDPIVEATQGMDRNT
                     PMTLASLTKPITATGILILVDQGKLRLDDPVSRYLPEFRMERHELGSAPITIRNLLQQ
                     TSGIPYAGRSPMVPSGVEGFFMPRQMYPAGMHHEYSNSNYELLGFIVERVTGMTFAAF
                     MQENLFGPLKMKNSRAAGVMSGASGITSTAEDLSHFAKMLLSWGRYEGMEVLSPELIR
                     EMFLPPPHIPVSENMSYYAMGWKVNVAHGRVVEAYHPGVWFNMLTDLRIYPTRKMFFV
                     TMSNPPFYRSDAATEFMGMTVGHGRRAVAYMGPSEDLEARPTQPDAGILNLYSGQYAN
                     SDGAVLRIAVQGKNIVRKMYQDERVLAPISQYEFTGDGNAMPHEFVWKNGQVAGLNTP
                     GGYYERRFPR"
     gene            4450..4890
                     /locus_tag="PLG78_11280"
     CDS             4450..4890
                     /locus_tag="PLG78_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE23744.1"
                     /translation="MRFKNIIFPTDFSESSLHALHVADEIADKFNSQLQILHVIYDMV
                     AYADAAAVMNWYPAMYVDLEKAAKEELHKVAKKVKFAKDPQLVVRRGVEETEIIRYAD
                     EQKADLIVMGTHGRTGVGRLLLGSTAEQVLRHAHCPVLTVRSDT"
     gene            4906..>5211
                     /locus_tag="PLG78_11285"
     CDS             4906..>5211
                     /locus_tag="PLG78_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE23745.1"
                     /translation="MWITYALLSAFFAALVAIFAKLGMSKVDSDLATAIRTVVILVLA
                     WSIAWFRGKLDGIVNLDRSAILFLVLSGLATGFSWLFYFRAMQMGKVSQVAPIDKLSL
                     "
BASE COUNT         1269 a         1348 c         1306 g         1288 t
ORIGIN      
        1 aggcatattg cgatatccag ccatcgcatt ggattgaatg acacgcgcga gtccacagat
       61 cctttcgcac acaacgggat tgcgcttgtg aggcatggca ctcgaacctt tttgtcctgc
      121 tctgaaaggc tcttccactt cgcgcgtttc cgtcttctgg agcaacctga cttcttgcgc
      181 catccgatcg agactccccg caatcactgc gagcacagac tgaaagtaag catggcgatc
      241 gcgactgata acctgagtag aaattggatc tggcttaagg ccaagatctg cgagcacttc
      301 ttcttctatt tcaggatcat tggcagaaaa tgttccaact gctcctgaca tttttccaac
      361 cgctacctct tcgcgcgcgc gaagcaggcg tgcgcgattg cgcttcatct cttcatagaa
      421 gagcgcaaac ttcaatccga gcgtggttgg ctctccatga attccatggg ttctaccaat
      481 catcacctgg ttcttgtgtc gaatggcgag agtcctggca gtgtctacga gttcatcgac
      541 tcgcttgagt aaaatgtcag cggcctgcac catctgcagg gacaacgctg tgtctccgag
      601 atcagacgaa gtcattccat agtgaagatg tcttcctgcc tctccaatgt gatcctttac
      661 gtttgttaga aatgcaatga cgtcatgctg gactacttct tcaatctcaa ggatcttcgc
      721 agtatcaaag ccggccttct gttgaatttc cttccagtct tgatccggaa cctggcctcg
      781 cttgtgaaga gctcgcgtga ccgccagctc cacatccagc catatgcgat atttattgtc
      841 gagttcccag atcctggaaa tctctggatt tgaatagcga tctatcatac gttatgcgct
      901 cttgtttgga atggaatgta ccgcttcttt ctcatgatgg tttgcaaact ttatgaatct
      961 ctctacctgg tcgcgaagga ggccaaagag gaattcatct gtgatacgat tgtcctgcat
     1021 tttcttccct atgtgcggaa accagactct ctcgggaagg ccgtaggcgt tgcgatagtt
     1081 gcaaaccatt tgtagctgtt ccactgcgcg aaacccaccc cagagcccat ccgcaagacc
     1141 ggtgtaggca atgggtctgt gttccaggct ctccggaaat ttcagcatat caatgaagta
     1201 tttgagagca cccgggaagg atccgttgta ttccggaacc accatgtgaa ttcctttgca
     1261 tgccagaatt ctttcctgga agggagcaaa tgcggccggc tttgttttgt aggatgcggg
     1321 ctggtaaatc tcaactggaa gtgaggcaat atccagaatc tgcgatggcg cgccagcgag
     1381 gtcgtataga ccgcggatga tttgagccag ctgaattgtt tttgatccgg gccggtttgt
     1441 gcccgccacg atctcaatca taccgttcca gacttttcaa aggaagcagg ctgtcgcctc
     1501 atttcgaaaa caggaaatct atttctgtgg cgtatctgtg tcttcagggg gcttgcgaac
     1561 gtagtatccg tcataggctg caatcccaag caacttgttg gtgccgggct cgtgtacaaa
     1621 atcgtgggac aggtcctgaa atccgcctgt aaaaacatga ttggtctgag tgcccagagg
     1681 ataagcatac gtgcccgcgc gagagaaaaa aagcacatcg ttcttcgcag tcacaacagc
     1741 ctctcttcca gtgagcacgt tgatgtatgt tccagggtag gtttcaaaaa cttcaggcgg
     1801 tggcggagtt gctttgaata aatacagagg agtgtccgct ccggcatatg cggttgcaag
     1861 atcgcctaac tttgcctgaa gtgccagaag cattcccatt gtgtagtcgt cgtactttgg
     1921 aggatcaccc agatacagaa tcgccatgcg atatcttgga aacaattgaa gccaggcggc
     1981 aatgtagtct gctccaccaa tatggaagaa ggtaacaact ccctttggcc ctctcttcac
     2041 tctccacccg agcccgcaat attcctgaaa ttcagcagga ggataataaa ccggctgcgt
     2101 gagcattcga ttcactgtct cttcagataa tatagttttg cccccatatt tcccgtgatt
     2161 caaatacatc atggcgaaat gcgataaatc ttcaatcgtc gtggccactc cagacgctcc
     2221 ggtaagctgc gatgtcttgg tctggcgcat ctcgagtggc tcaaagatct cttcgcgcat
     2281 gaattgatcc atgggctttc cgcttctgcg cgaaaccatt tccccgagga tcataaagcc
     2341 ggtgttagaa tatcgatagt gcatccccgc aggaacgatc tgcggcggaa tgcgaaaggg
     2401 cagttccgga gatgcaatca actcaggatg aaagtagcga aggtctggca tcccagaagt
     2461 gtgcgaaagc aaatgccgaa tcgtcactgg cctggaggaa agttcgggcc tctcaatgcg
     2521 aacttccata taatcggtga gcggcgcatc gaggtccacc gccccctcct ccacgagctt
     2581 catgactgcg gtggcagtga agatcttcgt gatcgacccg agcgcgtatt ttctgtcctg
     2641 ggtcatgccg gtctcgtgct tgtagacaag tttgtctgcc ttgaccaccc cgaccgtcat
     2701 cccatagaac tgtccgcggg ccctccagta ggccagaaag ctctccagct gaccctggat
     2761 ttgggcgatt ctctcaggat tttctgggcc aaatggcact tcctcgattt ctggcgtctg
     2821 gcagccagaa caaatgacga ccagagcagt cgtggctgcc gccaaaaggt gcttgaaaac
     2881 tggggcaatt tgaaaaaaat gccctctctt cgagggaaaa gcgttcatcc gataataaaa
     2941 tggggcaaat gaggttctaa tgacatttcg gcatatacgc atcgcaggtt tcgccttcct
     3001 gggtattctt tttgtagtac caggactccg cggaccaggc ttctcccacc aggtggaagg
     3061 aattccgggt gtggagctca tcatgcaaga ggctagcccc tctgaatggg ccaaaaaacg
     3121 tctccgcaac attcaggaca ctctgcgcga ttttgttcac agccgcgcag gcagacccgc
     3181 actccccaat ggactggtca ttgtaaacaa tgtccacgaa aaagatccaa tcgtagaagc
     3241 aacccaggga atggacagaa acactcccat gacgcttgct tctttgacaa agccaatcac
     3301 tgcaacaggt attctgattc ttgtagatca aggcaaactc cgcctggacg atccagtgtc
     3361 tcgctatttg ccagaattcc ggatggaaag acatgaactt ggcagcgctc cgatcacgat
     3421 tcgcaatcta cttcagcaaa catccggcat tccgtatgct ggcagatcgc ccatggttcc
     3481 ttctggagtg gaaggtttct tcatgccgcg ccagatgtat cctgcaggca tgcatcatga
     3541 gtattccaac tccaactacg aactgctggg cttcattgta gagagagtca caggaatgac
     3601 tttcgccgcg ttcatgcagg aaaatctgtt tgggccactg aaaatgaaaa actcaagggc
     3661 tgcgggagtg atgtcaggcg catccggaat cacaagcacc gcagaagatc tttcgcactt
     3721 cgcgaagatg cttctgagct ggggaagata cgaagggatg gaagtgcttt ctcctgaatt
     3781 gattcgtgag atgtttcttc ctccaccgca cattccagtt tcagaaaaca tgtcctacta
     3841 cgcgatgggt tggaaagtaa acgtggccca cgggcgagtc gtagaagcgt atcatcctgg
     3901 agtatggttc aacatgctta cggaccttcg catctatcca acgcgaaaaa tgtttttcgt
     3961 aactatgtcc aatccgccct tctatcgctc ggacgcggcc actgaattca tgggaatgac
     4021 cgtaggacac ggccggcgtg ccgtcgctta tatggggcca tcggaagatc tagaggcaag
     4081 acctactcag ccagatgcgg gcattctgaa tttgtacagc gggcagtatg caaatagcga
     4141 cggagcagta cttcgcatcg cagtgcaggg caagaatatt gtacgcaaaa tgtatcagga
     4201 tgagcgagtg cttgctccca tttcacagta cgaattcaca ggagacggca atgccatgcc
     4261 tcatgaattc gtctggaaaa atggtcaggt agccgggctc aatacacctg gcggctacta
     4321 cgaacgccgt tttccgcgct aatttccaca cttccggcca tcctgacaaa agtcataggg
     4381 cttcagtgac agcaaatcta ccttgcccga tccgggcatg gtagactaag cttacatttg
     4441 gaggccacca tgcgctttaa gaatatcatc tttccaacgg acttttcaga aagctccctg
     4501 cacgctctcc acgtcgcaga cgaaatcgca gacaagttca actcacagct gcagatcctt
     4561 cacgtgatct atgatatggt ggcctatgca gacgccgcgg cggtgatgaa ctggtatccg
     4621 gccatgtatg tggatctaga aaaagcggca aaagaagaac tgcacaaagt tgcgaagaaa
     4681 gtaaaattcg caaaagatcc tcaactggtt gtgcgacgcg gcgtagaaga aacagaaatc
     4741 attcgatacg cagacgaaca aaaagcggat ctcattgtga tgggaactca tggacgcaca
     4801 ggtgtgggca gattgcttct tggaagcacc gcagaacagg tactgagaca tgcccattgc
     4861 cccgtgttga ctgttcgttc tgacacttga tccacggcca aacttatgtg gatcacctac
     4921 gcgcttctct ccgccttctt tgcggcgctc gttgccattt ttgcaaaact tgggatgagc
     4981 aaggtggaca gcgatctggc cacagccatt cgcacagtgg tgatcctggt gctggcctgg
     5041 agcatcgcat ggtttcgcgg gaagttagac ggcattgtga atctggatcg aagtgccatc
     5101 ctgtttctgg tgctttctgg tctggccacc ggattctcct ggctctttta ctttcgcgcc
     5161 atgcaaatgg gaaaagtttc tcaggtggcg cccattgata aactcagcct c
//
LOCUS       DAOQJL010000246         5209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9279_length_5209_cov_4.456539, whole genome shotgun sequence.
ACCESSION   DAOQJL010000246 DAOQJL010000000
VERSION     DAOQJL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_5209_cov_4.456539"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLG78_11290"
     CDS             complement(<1..777)
                     /locus_tag="PLG78_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23746.1"
                     /translation="MNELTSCLPPVLIRNQDAIRENLETALGTLPTDLALSFESDLKR
                     AADGSLTRWLFQMICLHSGFASVTLFAGPIAIFSYGIQSDSRTMPPDVWTEIDLPFIV
                     GEHRLRFYGILPIKNKPFATQASDSLLVHAENVFLNSHGLREKSIQNIFFHVEKELRP
                     LLEALLEQGTPVTLTHFLFQDLRVYFSLSGEYRSGEIMDSIQKTIKENLKNTDSIFRI
                     SPLSYIVLSPGADAAQIETRFKSIYVQIKSLVLDYHLKLFT"
     gene            complement(796..1782)
                     /gene="cysK"
                     /locus_tag="PLG78_11295"
     CDS             complement(796..1782)
                     /gene="cysK"
                     /locus_tag="PLG78_11295"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNE23747.1"
                     /translation="MKIFEDNTLTIGRTPLVQLNRVAAGKGARVIAKIEGRNPAYSVK
                     CRIGAAMIWDAEKKGKLKKGMEIVEPTSGNTGIALAYVAAARGYPLTLTMPESMSIER
                     RKVLKAFGAKLVLTEAAKGMKGAIEKATEIANSDSAKYFMPQQFDNPANPAIHEETTG
                     PEIWQDTEGKIDIFVAGVGTGGTITGVGRYLKKQNKAIQIVAVEPAHSPVLTQTMKGE
                     KVAPGPHKIQGIGAGFVPSIVDISLIDRVEQVTNDESIEMARRLAKEEGILCGISCGA
                     AATAALRLASLPENAGKNIVVILPDSGERYLTTALFEGLFSGIDAAAAGAVI"
     gene            complement(1817..2869)
                     /locus_tag="PLG78_11300"
     CDS             complement(1817..2869)
                     /locus_tag="PLG78_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNE23748.1"
                     /translation="MSANKKSPVARRPSPGKLPAKEKAKKPSTQADTKFARNLKKLLS
                     LIPILKANQGIKIKDLQKISGYTSEKKLHEDLEKLLFFGQPPFTPSDYIDIYIEDDRV
                     FLEFPQGLDRPLALTPDEWTAVQALLEKEINFYSGGRKPTKHLRDIVLRMSQVPLAIE
                     PGPFARKQSILMRAIDDSLQVEFQYRSLYSKEPEIRRIDPWAVFANQGTIYCLGYCHT
                     RRSPRIFHLERMEKVEILNVKQEKAPSVQPVLNESFIFRKDKSGFSGEIEFDRGVRPA
                     LERTLDLKEIKKGAGGRWTAKVNIKDSHWFKAMIRSFGPYVKILSPDHLRQSFISELR
                     EIPVPEAFEVDGKSNS"
     gene            complement(2866..3903)
                     /locus_tag="PLG78_11305"
     CDS             complement(2866..3903)
                     /locus_tag="PLG78_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNE23749.1"
                     /translation="MPGTREEGMAKMERLGCLYLTFLNHPNGLSFSKIRDNLAPAYSG
                     DPESARRKFERDKDELRLLGLDLEHFPDGQALPNGNLARGHIYVPRNEFEKLPDLKMS
                     AEEASALSAVLLYAIENTKDERNAELYRSAATKLLYRDPSLLSRGSSQDFWAPSESFV
                     DHLAPIRSAIRKRLLLEIEYPDKELGSSIRLVEGRGLISHRGRWCLVGFARDANAIRY
                     FYLDRIVSVNVTKTQFRADPRFNIKEHTLHPLTLSIHEAAVIDLLLHEEGKEAMVDFL
                     SGIPDNRVKFGNEKNAARIQLRIKTTNKEALFSFMFRNPGVVVQIGPEDIRKDYLNYI
                     ARIRSQYGANP"
     gene            3991..4980
                     /locus_tag="PLG78_11310"
     CDS             3991..4980
                     /locus_tag="PLG78_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23750.1"
                     /translation="MSVVRGLEFLGRTQLMRYGRTEKTSLQIPVRGESLPAYRYSPAS
                     RLRGTILMVHGMTLRGIDDPRLENLGRSFASMGFEMVAPLFPEVRDLRITAETVSKIE
                     DAVTWLSEDVQGSVALFSASFSGGLSIVAACRAIKNKLSAVCTVGTLGDVDSTLDFLL
                     GQQGIDDYGTLVVLWNFLPVPEDVRTALYIAAADNGLIREPPELGRFVAGMKSTEREL
                     FERLRQDPAFRMERWKEFSSTSQFRELRQNVSPLLHVGKMPFRLVLVHGEADNVIPKE
                     ESEKLDRAFREAGTETQLCVTPLISHGDGSIGLSAAPQAWKLASAMGSFFSAI"
BASE COUNT         1149 a         1222 c         1354 g         1484 t
ORIGIN      
        1 tgtgaatagt tttagatgat agtccaggac caggctcttg atttgcacgt aaatgctttt
       61 gaatctggtt tcaatctgag ctgcgtctgc tcctggagat aaaacaatgt agctgagagg
      121 agagattcga aatatactgt ctgtattctt taaattttct ttaatagttt tctggatgga
      181 gtccatgatt tcgccgcttc tgtattctcc tgaaagagaa aaatatacgc gcaaatcctg
      241 gaatagaaaa tgagtcagag taaccggagt gccctgttcg agcaatgcct cgaggagagg
      301 gcgcagttct ttttccacgt gaaagaaaat gttctgaatg cttttctctc tgagtccgtg
      361 tgaattgagg aacacgtttt ctgcgtgcac gagaagtgag tcgcttgcct gagttgcgaa
      421 cggtttgttt ttgatgggaa ggattccgta gaagcgaagt ctgtgctctc ctacaatgaa
      481 tggtaaatct atttcagtcc aaacatccgg aggcatggtg cgcgagtcgc tctggattcc
      541 atatgagaaa atggcaatgg gtcctgcaaa cagcgtgacg cttgcaaatc cgctgtgaag
      601 gcagatcatt tgaaaaagcc agcgagtgag ggatccgtct gccgctcttt tgagatcact
      661 ttcaaaagaa agcgccaggt ctgttggaag agtacccagc gcagtttcaa gattttcgcg
      721 aatggcatcc tgatttcgta tcaggacagg gggcaggcag gatgtaagtt cgttcactgc
      781 cccctcccgg agtagttaga ttacggcgcc ggctgcagct gcatcaatgc cgctgaacaa
      841 tccttcgaaa agtgccgtcg tcagataacg ttcgccagag tcaggaagga tgacaacgat
      901 attcttgcct gcattctctg gcaacgaagc cagtctgagc gctgcggttg ctgccgctcc
      961 gcaggaaatg ccacagagga ttccttcctc ttttgccagt ctgcgcgcca tttcaatgga
     1021 ttcgtcattg gtcacctgtt ctactcgatc aatcaggctg atgtccacaa tggatggaac
     1081 aaaacctgcg ccgattccct ggatcttgtg cgggcctgga gctacttttt cgcccttcat
     1141 agtttgcgtg agtaccgggg agtgtgccgg ttccactgct acaatctgga ttgccttgtt
     1201 ttgctttttg agataacggc ccacacctgt gatcgttccg cctgtgccaa cgccagcgac
     1261 gaagatgtca atcttgcctt ctgtatcttg ccagatttca ggaccggttg tttcttcgtg
     1321 gatggctgga tttgcaggat tgtcaaactg ctgaggcatg aaatacttcg ctgagtcaga
     1381 gtttgcgatt tctgtcgcct tttcgatggc tcctttcatt cccttggcgg cttctgtcag
     1441 aaccagtttg gcgccaaatg ctttgagaac tttccggcgc tcgatggaca tgctctctgg
     1501 catggtgagt gtgagcggat agccgcgagc ggctgccaca taggccaggg caatgcctgt
     1561 gtttccagaa gtaggctcta cgatttccat tcccttcttc agctttcctt tcttctctgc
     1621 atcccagatc atggctgcgc cgattcggca ttttacagag taagccgggt ttctcccttc
     1681 aattttggcg atcacgcgcg cgcctttgcc tgccgctact cggttcagtt gaaccagggg
     1741 tgtgcggcca atcgtaagtg tattgtcctc gaagattttc ataaagacct cattgtctag
     1801 tgtttatatc aggatactag gaattgcttt ttccgtcaac ttcaaatgcc tctggaaccg
     1861 gtatctcgcg caactcggaa atgaagctct gtctcaggtg atccggagaa aggatcttca
     1921 catacggtcc aaaagaacga atcattgctt tgaaccagtg gctatctttg atgttcacct
     1981 tcgccgtcca tctgccaccc gcaccttttt tgatttcttt gagatccagg gttcgctcca
     2041 gagcaggtct cacaccccta tcaaattcta tttctccaga aaagccgctc ttgtccttgc
     2101 gaaagataaa agattcattc agaactggtt gaacggaagg agctttctcc tgcttcacgt
     2161 ttagtatctc tactttttcc attctttcca gatgaaagat gcgcggagaa cggcgagtgt
     2221 ggcagtatcc gaggcaatag atggttccct ggttcgcaaa cacagcccac ggatcgatcc
     2281 gacgtatttc cggttctttt gaatagagac ttctgtactg aaattccacc tgaagcgaat
     2341 cgtcgatggc tcgcatgagt atggattgct ttcgcgcaaa tggtccgggt tcgatggcaa
     2401 gcggaacctg actcatgcga agaacaatgt ctctcaaatg cttggttggt tttcttccgc
     2461 cgctatagaa gttgatttct ttttcgagta gagcctgaac tgcggtccat tcgtccggtg
     2521 taagagccag aggacgatcg aggccctggg gaaactccag aaagactctg tcgtcttcaa
     2581 tgtaaatatc aatgtagtca gaaggagtga agggcggctg accaaagaat aatagttttt
     2641 ccaggtcttc gtgcagcttc ttttcgctcg tatagccgga gatcttctgc aaatctttga
     2701 tcttgattcc ctgatttgct tttagaattg gaatcagaga aagaagtttc tttagattgc
     2761 gcgcgaactt tgtgtctgcc tgcgtggatg gcttctttgc tttttctttc gcgggcagtt
     2821 tcccgggaga gggacggcgt gccaccggag atttcttgtt tgcgctcatg gattggctcc
     2881 atactgactg cgaatgcgcg caatgtaatt caagtaatcc tttctgatat cttcaggacc
     2941 aatctgaacg accacacctg gattgcgaaa catgaaactg aagagcgcct ctttgtttgt
     3001 tgttttgatt ctgagttgaa tgcgtgctgc attcttttcg tttccaaact tgaccctgtt
     3061 gtccggaatc ccggacagaa agtcgaccat ggcttccttc ccttcctcgt gaagcagtag
     3121 atcaatgact gcggcttcgt gaatggacag agtgagcgga tgaagggtgt gctctttgat
     3181 attgaatctt ggatctgcgc gaaactgggt tttagtcacg ttcacggaaa caattctatc
     3241 cagataaaag tagcgaattg catttgcgtc gcgagcaaaa ccaaccaggc accagcgtcc
     3301 tctgtgtgaa atgagtccgc gcccttctac caggcgaatg ctcgatccca gttctttgtc
     3361 tggatattca atctcaagaa gcaatcgttt gcggatggcg gaccgaatgg gcgccagatg
     3421 gtccacgaag gactcggaag gggcccagaa atcctgagaa cttccacggc tcaggagaga
     3481 tggatcgcgg tagagcagct tggtggcagc ggatctgtaa agttctgcat ttctttcatc
     3541 ttttgtattt tcaatcgcat acagtagaac cgcagacagc gcgctcgctt cctctgcaga
     3601 cattttgaga tccggcaatt tttcaaattc gtttcgcgga acgtagatat gtccgcgcgc
     3661 aaggtttccg ttcggaagag cctggccatc cggaaaatgt tctagatcca gacccagcaa
     3721 tcgcagttca tctttgtctc tttcaaactt tcttctagcc gattcaggat ctccggagta
     3781 tgcgggggcc agattgtcgc ggattttaga aaaactcagt ccatttggat gattcaagaa
     3841 tgtcaggtag agacatccga gtctctccat ctttgccatt ccttcttcgc gagtgcccgg
     3901 catgcctggg taaggcacat cgccagtgtt gtcggtcaag gaaaaagaac aaaatgattg
     3961 cgagcgcgct atgctctcta gactttcggc atgtctgttg ttagagggct tgagtttctg
     4021 ggcagaacgc agttgatgcg ctacggtaga acggaaaaaa cctctctgca gattccagtt
     4081 cggggagagt ctctgcccgc gtatcgttat tctccagctt ctcgcttgcg cggtaccatc
     4141 ctcatggtcc atggcatgac tcttcgcgga attgatgatc cgcgtctgga gaacctgggt
     4201 cgatcttttg cgagcatggg gtttgagatg gtggctcctc tttttcccga ggtgcgcgac
     4261 cttcgcatca cagcagaaac tgtttctaag atagaagacg cagtgacctg gctttcagaa
     4321 gacgtgcaag gaagtgtggc tcttttctct gcttcttttt ctggcgggct ttccattgtg
     4381 gctgcttgca gagcgattaa gaataagctg agtgctgttt gtactgtggg cactctgggt
     4441 gatgtggatt ctactctgga ctttcttctg gggcagcaag ggatcgatga ttatggcaca
     4501 ctggttgtgc tgtggaattt tctgccggtg ccagaagatg ttcgcactgc tctctatatc
     4561 gcggctgcag acaatggact catacgcgag ccgcctgaac tcggaagatt tgtggcaggc
     4621 atgaagagca cggaacgaga actgtttgaa agactcagac aggaccccgc gtttcgaatg
     4681 gaaaggtgga aggagttttc aagcacctct cagttcagag agctcaggca gaacgtttct
     4741 cctcttttgc acgtgggtaa gatgcctttt cgcctcgtgc tcgttcacgg agaagcggac
     4801 aacgtaattc caaaagaaga atcagagaaa ttggaccggg cttttagaga agcaggtacg
     4861 gaaacgcaac tgtgcgtcac tccgcttatt tcgcacggcg acggttcgat cgggctttct
     4921 gccgcgccgc aggcctggaa gctcgcttct gcgatgggat cttttttttc tgcgatttag
     4981 gttttgcagc gggcttcgtg gttttctttc gctgagccga ttttgttttc tttctggtgg
     5041 gctttggatc tggttttgcg gatttgtgaa ttgtgtctga tagactgcgg tcttcgctga
     5101 ggatggcagt gcgaagagat tctgccagtt gttctttttc ctccggcaat gcagcaagca
     5161 cgctgtcttt ctgagaaatt tcgccagatg gtttgcgcga agccatcag
//
LOCUS       DAOQJL010000247         5201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9311_length_5201_cov_3.362029, whole genome shotgun sequence.
ACCESSION   DAOQJL010000247 DAOQJL010000000
VERSION     DAOQJL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9311_length_5201_cov_3.362029"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2642)
                     /locus_tag="PLG78_11315"
     CDS             complement(<1..2642)
                     /locus_tag="PLG78_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNE23751.1"
                     /translation="MSQTTENKQKHYWQTLEERTQPQSFAIYEKPEFTQDRKDMLEEA
                     ETLKVSRKTFLKVMGAGAVMAQAACRRPTEQIVPAVIQPPEIVPGIPVFYSTVAPSGS
                     GMIVRTREGRPIKLAGNADHPVNQGGLTASDVASIMDLYDPDRLRKAVRIKDGKKRNV
                     AAELLITEIKTNLGKGDYVLLTAPTISPATRSIVRDFMAQFPGGRQLTFRPDPTLRQI
                     AEGSQASYGKQVIPDYRFDKADYILSIDGDFLGTMIQPAHFTSLFAKGRELRRGQKTM
                     SRLVAIESMFSTTGSNADERIAIRPGDQVVVALSIAAQLAIKLGVNAPGGSGALEKYL
                     PAKVASSLAASPEQASKMQESLERIAKELSEVRGSSLVIGGSPLAATGSNASLAIAVN
                     LLNSMLGNDGKTVDHVAALQLDPGATDREIQDLIQQMNSGKVQSVIVIGANPVYHLPE
                     SYKFAEALKKVPYSAAIQDRIDETAKLTQAVLPVNHYLESWGDAELLPGVYSIQQPVI
                     RPLWATKSAEDYLIAIAGGSIKGASTFHQYMKSQWEGRARGDFRKFWQSSLQTGYFAP
                     GAADLGRDRGARNFNAGSLSQITQPEKTSGFKLGLYYNVQVLDGSGANNSYRQELPDP
                     VTKVVWENYVAILPDTGRKLKLKQGSVVTVKTAKGSFELPVHLQPGLHPEAVFIALGY
                     GRTDAGLTGNGQGKNAIALVSPGSDSIAYSGIKVDIEPTGDRYKLPNTQSVYRNGFNT
                     EDKAFFAPEGLPGAPMAGASQNGRPIIREATLKEYIEKPGEFKPEGVDYPGKDPSKKE
                     EAARLMKEWAYNGTKWNMAIDLTACTGCGACVTSCNSENNIPMVGPEEVARGREMHWL
                     RIDRYYSGDENSPEVAHQPMMCQH"
     gene            complement(2658..3236)
                     /locus_tag="PLG78_11320"
     CDS             complement(2658..3236)
                     /locus_tag="PLG78_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE23752.1"
                     /translation="MSLIRIFQKHGVKLFVIISLISGIAYLFAADAKYQGYAPDQPIP
                     FSHKIHAGELKISCQYCHSGVEVSAHATVPDQGTCMKCHSVVASDSPNIQYLRQTYKQ
                     GVPIRWVKVHDLPDHVRFSHRPHIARGFDCNQCHGDDTRGLKVEEMDKIQVTTKFNMG
                     WCIKCHRDNAVQEHPPGNNHDVKITECSTCHY"
     gene            3537..>5201
                     /locus_tag="PLG78_11325"
     CDS             3537..>5201
                     /locus_tag="PLG78_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNE23753.1"
                     /translation="MKLDKLTTKATEAISQAQEEARKRTNPEAMPEHLIYEIFRQENG
                     MGPVILQRLGMQEASVHRILTDYIDKLPKTQGDIDVRPSRAFIKLLEAGEALAQKRGD
                     QFLSTEHILLAYIQDSSQKLTREMGALGLTADAVSQVLDKLRAGKPVTSENPEDSTEA
                     LSKYARNLNDSARKGKLDPVIGRDEEIRRIMQVLSRRTKNNPILIGEPGVGKTAIVEG
                     LAGKIVQGEVPDGLKDKEIYALDLGSMIAGAKYRGEFEDRFKALITEVQASEGRVILF
                     IDELHTLVGAGAAEGSLDASNMIKPALARGELRCIGATTLKEYQKYIEKDQALERRFQ
                     PVYVNEPSVDDTIMILRGLKDRYELHHGIRITDGAILGAARLSDRYITDRFLPDKAVD
                     LIDEAMSKMRIELDSMPEELDKIAKRVQNLKIEREAIKMEKDTASKERLRLLEKELAD
                     VESEFRTKKGIWDSERIDVEKAKKIREQIDALRIQEKEAERKGDLNQVAEIRYGKLTA
                     LEAELKKAEANIEKAQKQYLREEITFEDIAGIVARWTGIPVSRMMQG"
BASE COUNT         1273 a         1235 c         1402 g         1291 t
ORIGIN      
        1 caatgctggc acatcatggg ctggtgggcc acttctggag agttttcgtc tcctgaatag
       61 tagcgatcaa ttcgcagcca gtgcatttcg cggccgcgtg ccacttcttc cgggccaacc
      121 atgggaatgt tgttttcgct gttgcagctg gtaacgcagg cgccgcagcc tgtgcaggct
      181 gtaaggtcga tcgccatgtt ccacttggtt ccgttgtaag cccattcctt catgaggcga
      241 gcagcctctt ctttcttgga aggatctttg ccagggtaat ccacaccttc tggtttgaat
      301 tcgcctggtt tttcgatgta ttctttgaga gtagcttcgc gaatgatcgg acgtccattc
      361 tggcttgcgc ctgccatagg ggctccaggg agaccttccg gtgcgaagaa tgctttgtct
      421 tctgtgttga acccgttgcg atatacagac tgagtgttag gcagcttgta acgatctcca
      481 gttggttcaa tgtctacttt gattccgctg tatgcaatgg aatcagatcc aggagatacc
      541 agagcgattg cgtttttgcc ctgaccattt ccagtcaggc ccgcgtctgt tcttccgtaa
      601 cccagtgcaa tgaacactgc ttctggatga agtccaggct gaagatgaac tggaagttca
      661 aaacttccct tcgcagtttt tacagttact actgaacctt gtttgagttt cagtttgcgt
      721 cctgtatcgg ggagaatcgc aacgtagttt tcccagacca cttttgtaac tggatctgga
      781 agttcctggc gataggagtt gtttgcacct gatccgtcta gaacctgaac gttgtagtaa
      841 agacccagtt tgaatccaga agttttctca ggctgagtga tttgggagag ggaaccagca
      901 ttgaaattgc gtgcgccgcg atctcgtccc agatccgcag cacctggagc aaagtaacca
      961 gtttgaagag aagattgcca gaacttgcgg aagtctccgc gtgcacggcc ttcccactga
     1021 gatttcatgt attgatgaaa cgttgaggcg cctttgatgg atccaccagc gatggcgatg
     1081 aggtagtctt ctgcggactt tgttgcccag agaggacgaa tcacaggctg ctggatggaa
     1141 taaacaccgg gaagaagttc tgcatctccc cagctttcca ggtagtgatt cacaggcagc
     1201 actgcctgag tgagctttgc agtttcatca attctgtctt gaatggcagc agaatatgga
     1261 actttcttca atgcttccgc aaacttgtaa gactctggca gatgatagac aggatttgcg
     1321 ccaatcacga tgacggactg cactttgcct gaattcatct gctgaatgag gtcctggatt
     1381 tctctatccg ttgcacccgg atcgagctgc aatgctgcaa cgtgatccac agtctttccg
     1441 tcatttccaa gcatggagtt gaggaggttc actgcgatgg caagagaagc attgcttcca
     1501 gtggccgcaa gaggggagcc gccgatgacg aggctacttc cgcgaacttc tgagagttct
     1561 tttgcaattc tttcgagaga ctcttgcatc ttgctcgcct gttctggaga tgcagcaaga
     1621 gagcttgcga ctttcgcagg cagatatttt tcaagtgcgc ctgatccgcc tggagcgttg
     1681 actcccagtt tgatggcgag ttgcgctgca atagaaagtg caacgactac ctggtcgccc
     1741 ggacgaatcg caatgcgttc gtctgcgttg gatccagtgg tgctgaacat actttcgatc
     1801 gcaacgagtc tgctcattgt tttttgtccg cggcgaagct cgcgcccttt cgcaaacaga
     1861 gaagtgaagt gagcaggttg aatcattgtg cccaggaaat cgccgtcgat ggatagaatg
     1921 taatctgcct tgtcgaagcg atagtcaggg atgacttgtt ttccgtagga agcctgggaa
     1981 ccttctgcga tctggcgaag agttggatcc ggacggaaag tgagttgtct tccaccgggg
     2041 aactgcgcca tgaaatcgcg aacaatggat ctggtagcag gactgatggt cggggcagtc
     2101 agaagaacat agtctccttt gcccaggttt gttttgattt cagtgatcag tagttctgct
     2161 gcaacgttgc gtttctttcc gtctttgatg cgcactgctt tgcgaagacg atctggatca
     2221 tacagatcca taatggatgc aacatccgat gcagtcagac cgccctgatt gacagggtga
     2281 tccgcgttgc ctgcgagttt gatagggcga ccttcgcgtg tgcgaacgat cattccgctt
     2341 ccgcttggag caactgtgga atagaagact ggaatgccag gaacaatctc aggcggctgg
     2401 attacagcag gaacaatttg ctccgttgga cggcggcatg ccgcctgcgc catcacagca
     2461 cctgcgccca tcaccttgag aaaagtcttg cggcttactt tgagagtttc cgcttcctca
     2521 agcatgtcct ttctatcctg agtgaattca ggcttttcat agattgcaaa cgactggggt
     2581 tgcgtacgct cttccagtgt ttgccagtag tgcttttgtt tattttccgt cgtctgactc
     2641 atttgttgtt ccggctctta gtagtggcag gtactgcact ctgtgatttt cacatcgtga
     2701 ttgttccctg gtgggtgttc ctggactgca ttgtcacggt ggcacttgat gcaccagccc
     2761 atgttgaact tggttgtgac ctgaatctta tccatctctt cgactttcag ccctcttgta
     2821 tcatctccgt ggcactggtt gcagtcgaaa ccgcgcgcaa tgtgaggcct gtggctgaaa
     2881 cggacgtggt cagggaggtc gtgtactttc acccagcgaa tgggtacgcc ctgcttgtaa
     2941 gtctggcgta gatactgaat gttcgggctg tccgacgcga ccacggaatg acacttcatg
     3001 caagttccct gatccgggac agtggcgtga gctgaaacct caacaccgga atggcagtac
     3061 tggcagctga tcttcagctc ccctgcgtga atcttgtggc taaacggaat gggctggtcg
     3121 ggagcatacc cctgatactt cgcatctgcg gcaaagagat aagcgatgcc gctgatgagg
     3181 gagatgatta cgaacagctt aacgccgtgt ttttggaaaa ttcgaatgag agacatagaa
     3241 gaaccagacg caaaaccttt ctatttgatt tttgctgacc tccgaccggg ttaagcaggg
     3301 cgactaaatt gtcaaagaaa tgtcaggcga tcacttcaaa aaattgcgcc ggttcatttt
     3361 tttcctcgcc gcacatcaaa aaattctgcc cgcttgagcc cgatcaactc ccatgacaaa
     3421 aagtctgagg tcatgacgaa tttgcttctc ctcggctttg catttttaag agaacttcta
     3481 tttgtaccct ttccgggccg gcaccaatcg tgcacgacag ggttcgggag cagtgcatga
     3541 aactcgacaa acttactaca aaagcgaccg aagcgatttc gcaggcccag gaagaggcgc
     3601 gcaaacgcac aaatccggaa gcgatgccag agcatttgat ctatgagatc tttcgtcagg
     3661 aaaatggcat gggtcccgtc atactccaga ggctgggaat gcaggaggcg agtgttcatc
     3721 gcattctgac ggattacata gacaaactcc cgaagaccca gggggacatt gacgttcgac
     3781 cttcgcgtgc attcatcaag ctgctcgaag caggggaggc gctggcgcag aagagaggag
     3841 atcagtttct ttctacggag cacatacttc ttgcctacat ccaggactct tcgcagaagc
     3901 ttactcgcga aatgggagca ctggggctca ctgcagacgc ggtatcccag gtcctggata
     3961 aattgcgcgc gggaaaacct gtcacgagcg agaatccaga agacagtacg gaggcactct
     4021 ctaaatacgc gcgaaatctc aacgactctg cgcgaaaagg aaaactggat cctgtcatcg
     4081 gaagggacga agagattcgt cgcatcatgc aggtactttc gcgaaggaca aagaacaatc
     4141 ctatcttgat tggtgaacct ggagtcggca aaactgcgat tgtagaaggc ctcgcaggaa
     4201 agatcgttca gggagaagtg ccggacggac tcaaagacaa agagatctat gcccttgatc
     4261 tgggaagtat gattgcaggc gcgaagtatc gcggagaatt tgaagatcgc tttaaggctc
     4321 tcatcacgga agtgcaggct tcggaaggaa gagtgattct gttcattgac gagctgcata
     4381 cactggttgg cgcgggcgcg gcagaaggat ctctagacgc atccaacatg atcaagcccg
     4441 ctctcgcgcg cggagagctt cgttgcattg gtgctactac tctcaaggaa tatcagaagt
     4501 acattgaaaa agaccaggct ctagaacgta gattccagcc tgtgtacgtc aacgagccat
     4561 ccgtggatga tacaatcatg atcttgcgcg gtctgaagga tcgctacgaa ttgcatcatg
     4621 gcattcgcat cacagacgga gcgattctgg gcgcggccag actttcggat cgctacatca
     4681 cggatcggtt tcttccggac aaagcagtgg acctgatcga cgaagccatg agtaagatga
     4741 gaatcgaact ggattccatg ccggaagaac tggataagat cgcaaaacga gttcagaatc
     4801 tgaagatcga acgcgaagcg atcaagatgg aaaaagatac tgcttccaaa gaacgccttc
     4861 gccttttaga aaaagaactg gcggatgttg aatctgaatt tcgcacgaag aaaggaatct
     4921 gggattcaga acgcattgat gtagaaaaag caaaaaagat ccgcgaacag attgatgcgc
     4981 ttcgcatcca ggagaaagaa gcggaacgaa aaggcgatct caaccaggtg gcggagattc
     5041 gttatggcaa attgactgct ctggaagctg agctcaagaa agcagaagca aacattgaga
     5101 aggcgcaaaa gcagtatctg agagaagaaa ttacattcga agacattgca ggcattgtgg
     5161 cgcgatggac gggaattcca gtctcaagaa tgatgcaagg c
//
LOCUS       DAOQJL010000248         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9321_length_5195_cov_4.208560, whole genome shotgun sequence.
ACCESSION   DAOQJL010000248 DAOQJL010000000
VERSION     DAOQJL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_5195_cov_4.208560"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..470)
                     /locus_tag="PLG78_11330"
     CDS             complement(60..470)
                     /locus_tag="PLG78_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23754.1"
                     /translation="MRNKLTLVLLSMLLSGFASAYAHEGHDKPDVLPPVGPHGGKYTK
                     MKNHFAEVVVQGDMAMIYILEDDIKSSPEKLSDVKASLEIPGKGNTTLKLSSMDGAFH
                     ASISIPKNVRRVYFQISAKVDGKLESGKVLYEPK"
     gene            complement(490..3669)
                     /locus_tag="PLG78_11335"
     CDS             complement(490..3669)
                     /locus_tag="PLG78_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23755.1"
                     /translation="MFTGKNHPIAGKKDLSHRIFDAVIAFSLERRRSVLAVAIILAAA
                     GTYTVMRLPVDVLPDLNRPAVSVLIESPGLAPEEVETIVTIPIESALLGGPGIGNVRS
                     QSNRGLAQVHVEFEAGSDIYRCRQIVKERIDSIASTLPRGTQIRLGPISSIMGEILLV
                     GITSKDGKNSPGDLRTFADWTVRKQLLSVRGVADITVIGGEAKQYQILIDKNKLSAYD
                     NSLFADNRYISFLAMKESLRGLGENTGGGFIEKEGKELSVRNLGNITDQSDLENSVIG
                     LRGGSPVRIKDIAVVSTGARVKRGDAGVNGNPAVILIIQKSPGVSTVDLTRSIATEIE
                     KIRKANDPDILIHDKLFRQADFIERAVGNVGEAIRDGSIIILIVVFLFLWSFRTTFIT
                     LVTLPLSLIAAIVTMAALGVDLNTMTMGGLAVAVGQLVDDSIVDMENIFRRLRENRNL
                     NAPRPALEVVFHASSEVRNSIVLATLVVILVFIPLFFLGDLEGRLFRPLAFAYIVALF
                     ASLLVALTVTPVLCYYLLRNASATQNETAIVRSLKAFQRRNLNALLDRPMLSTLIVGV
                     LFLISIGMAPFLKKDFLPSFNEGVFTVFGSLPTATSLPESVARMKEVEEVLLSTKGVG
                     QFARRTGRAEGDEHASGPHLTEIEVNVEPEKAGITRAKLLADLRTKFKSLKGIDLDIG
                     QPISHRIDHMMTGVRSEVAVFVYGDDLEAIQYQAYQIRSVLSKVPGITDLLIENQTKA
                     PELKIGINHAQASSYGITSGEITEVLEFALGGQTVGRTIENGKIFDIVLRFDDKSRAV
                     DGLSGIIIKYLPNGTPVRLGMVAEVYETEGPYEIKRENGSRRIAVQFNTQGRDLGSVV
                     SDVNAALKTLQMPQGIRYEIGGRFASRERAIRLIGIFGFCALVLLTFILYRNFKSWLL
                     VGQILVNVPFSFIGGIGALFVLGVPLSLASILGFIALTGIAARNGIMMISHFLHLMEV
                     EGMEFSRAMVIRGALERLVPVLMTAFSAVFALLPILVSGSDAAGKEILFPVALVISGG
                     LFSSTLLDIIFTPVIFYQVMKRAPNGASS"
     gene            4288..4974
                     /locus_tag="PLG78_11340"
     CDS             4288..4974
                     /locus_tag="PLG78_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23756.1"
                     /translation="MLITMRPGFRKFALTAHVTTSVGWFGAVGAFLCLAITGLRTTDA
                     HLMRATYLGMQLVAFCVIVPLSLASPLTGLVQSLGTHWGLFRHYWVLVKFLITVPCTI
                     ILLLHMQAIRALAQATASTTLNAIDVPALQVQMVVNAAVALVALLVATALSVYKPRGL
                     TPYGARSENRQDRSVLQSGDFARRPTFTQGKQSLSPRWVYVLGFVLIVIAAHIVILHI
                     SGGGLGGHTH"
     gene            5003..>5195
                     /locus_tag="PLG78_11345"
     CDS             5003..>5195
                     /locus_tag="PLG78_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNE23757.1"
                     /translation="MNAKGPKRGPGQPPISPGDPLKSTNVLLPGSYYKRIEKLVEAGK
                     FKTQSEAVRRMIEAGLKKFE"
BASE COUNT         1314 a         1328 c         1265 g         1288 t
ORIGIN      
        1 tcatataatg ctcctcaaaa aacggcgatc ctcacacagg ttggggatcg ccgttcttat
       61 cacttgggct cgtatagaac tttccccgat tccagcttcc cgtcgacttt cgcgctgatt
      121 tgaaaataca cgcgcctgac attctttgga atcgaaatgc tcgcatggaa agctccgtcc
      181 atggaactga gcttaagggt ggtgtttcct tttcccggaa tttcaagcga tgctttcaca
      241 tcactcagtt tctcgggaga actctttata tcatcttcaa gaatgtagat catggccata
      301 tcaccttgaa ctaccacttc tgcgaagtga ttcttcatct ttgtgtactt cccaccgtgc
      361 ggaccaaccg gcggaagaac atcgggtttg tcatggcctt catgcgcgta agcggaagca
      421 aatccgctca ggagcattga gagcagcacc aatgttagtt tgtttctcat gtttaacctc
      481 ttttgttttt tatgaacttg caccgttagg cgctcttttc atgacctggt agaatattac
      541 cggcgtgaaa ataatatcga gtagagttga gctgaataat ccaccggaga taaccagggc
      601 caccgggaag agaatttcct tcccggccgc gtcgctccct gaaacaagaa tagggaggag
      661 cgcaaagacc gctgagaaag cggtcattag aacaggtaca agccgttcaa gtgcaccacg
      721 aatcaccatt gcccttgaga attccatgcc ctctacttcc atcaaatgca ggaaatgaga
      781 gatcatcatg attccatttc ttgcagcaat gcctgttaac gcgatgaatc ccaatatgct
      841 tgcaagtgag agaggaacac ccaatacgaa cagtgcgccg ataccaccga tgaacgagaa
      901 tggcacattc acgaggattt gtccaacaag gagccaggac ttaaaatttc ggtacaggat
      961 gaaggtcaga agcacaagtg cgcagaatcc aaaaattccg atgagtcgaa tcgctcgttc
     1021 ccgacttgcg aagcgtccgc caatctcata gcgaatgccc tgcggcatct gcaatgtctt
     1081 gagggcggca tttacatcgg aaacgacaga gcccagatcg cgcccttgag tattgaattg
     1141 aaccgcaatg cgcctgctgc cgttttccct ttttatttca tacgggcctt ccgtttcata
     1201 aacttctgca accattccaa gccgaaccgg cgttccgttt ggcaggtact tgatgatgat
     1261 tccagagaga ccatctactg cgcgcgattt gtcatcaaat ctgagcacga tgtcgaagat
     1321 ttttccattc tcaatggttc ttccaactgt ctggccaccg agggcaaact ccagaacctc
     1381 ggtgatttct ccgcttgtaa taccatagga actcgcctgc gcgtgattga ttccaatctt
     1441 gagttcgggc gctttagttt gattctcgat gagtaaatct gtgatgcccg gaactttgct
     1501 caggacgcta cgaatctggt acgcttgata ttggattgct tcaagatcgt caccgtaaac
     1561 aaagactgcg acttccgaac gaacgccggt catcatgtga tcgattcgat gtgagatcgg
     1621 ttgtccgata tcgagatcga ttcctttcag tgatttgaat tttgttctaa gatccgcaag
     1681 aagttttgcg cgggttattc ctgccttttc cggttccaca ttcacttcga tctccgttag
     1741 atgcgggccg ctcgcgtgct catcgccttc tgcgcgtcct gtgcggcgcg caaattgtcc
     1801 cacacctttc gtcgaaagca ggacttcctc aacttctttc atgcgcgcca cggattcggg
     1861 cagcgaagtg gctgtgggca gcgagccgaa aaccgtgaac actccttcgt tgaaactggg
     1921 caagaaatct ttcttgagaa atggtgccat cccaattgaa atcagaaaca gaacaccgac
     1981 gatgagggtc gacagcatcg gacgatccag aagtgcgttc aggttcctgc gttgaaatgc
     2041 cttgagagag cgaacaatcg cagtctcatt ctgcgtagcg gatgcattgc gcagaaggta
     2101 ataacacaga acaggcgtta cagtcagggc aacaagcaac gatgcaaaga gtgcaactat
     2161 gtaagcaaac gcgagtggac ggaacaatcg tccttcgaga tcgcccagaa aaaagagtgg
     2221 aatgaaaacg agaatcacaa caagcgttgc aagaacgatg ctgtttctta cttcggacga
     2281 cgcgtgaaaa acaacctcga gggccgggcg cggcgcgtta agatttcgat tctctcgcag
     2341 acgcctgaaa atgttttcca tgtccacgat ggaatcgtct accagttgcc ccacggccac
     2401 ggccagaccg cccatcgtca tcgtatttaa gtcaacaccg agcgccgcca tggtgacgat
     2461 tgccgcaata agtgaaagag gaagagtaac gagtgtgatg aaggtagtgc gaaaactcca
     2521 caggaataag aatacaacaa tgagtatgat aatcgaaccg tctcgaatcg cttcgccgac
     2581 atttccgacg gctcgttcaa tgaagtccgc ttggcgaaat agtttgtcgt gaatcagtat
     2641 gtccggatca ttcgcttttc tgattttttc aatttcggtg gcaatgcttc gtgttagatc
     2701 aacggtagaa acgcccggtg atttttgtat gatcaggatc acggctggat ttccgtttac
     2761 gcccgcatca ccgcgcttca cgcgcgcgcc ggtagacacc accgcaatgt ccttgatgcg
     2821 aaccggactt ccacctcgca gtccaatcac agaattttcc agatcagact ggtccgttat
     2881 gttgccgaga tttcggacag aaagttcttt cccctctttc tcaatgaatc cgccgccagt
     2941 attttctccg agcccgcgca ggctttcctt catcgcgaga aacgagatgt agcgattgtc
     3001 cgcgaagaga ctgttgtcat acgctgaaag cttgttctta tcgatgagaa tttgatattg
     3061 tttggcttca ccaccgatga cggtaatgtc agctacgccg cgcacggaca ggagctgctt
     3121 gcgcaccgtc cagtcagcga aagttctcag atcacccggc gaattttttc cgtctttgga
     3181 tgttattcca acaagtagaa tttcccccat gatggagctg attggcccaa ggcggatctg
     3241 cgttccacgc ggcaaggtcg atgcaatgct atcgattcgc tcctttacaa tttgccgaca
     3301 cctgtaaata tcagatccag cttcaaactc aacatgcacc tgagcgagtc cgcgattcga
     3361 ttggctgcgc acatttccaa taccaggtcc accaagaagg gcggattcaa tcggaatcgt
     3421 gacaatcgtt tctacttctt cgggcgcgag accaggcgat tcgatcaaaa ccgaaacagc
     3481 cggtcgattc aaatctggga gaacatcgac aggaagtcgc ataacggtgt atgtacccgc
     3541 agcagctaga atgatggcga cggcaagaac cgagcgtcgg cgttccaacg agaacgcaat
     3601 gaccgcgtca aagatacgat ggctgagatc ctttttgccc gcgatcgggt gatttttgcc
     3661 tgtaaacacg actcctcctc aatcgatccg gccagacgca tgcgtggcgg aatcgaaaag
     3721 ctgtttaagc gagagaagga gttataggag gggcacgctg gcccagggtg aaacggattg
     3781 gcgcaggaag tctagtagta ccgtcccgta tcaggctata agacgccgat tcaacaacag
     3841 gcgggtatac aaatcgaact aagaaggtcg caatcagggc agaaagcaca agtaacacaa
     3901 ttgttgcgag ttcaagagga aggctcttgc tcagtacgag tttgagcaac gtcgctggaa
     3961 tctcatcctt tggaatactt ttgtcggagt gattgtgtgt aacccctttg ctgtgactgt
     4021 gcacagtccc attattatgt acatgaacgc caatcacagg gctcaacagg atgaaagaga
     4081 aggttgcgac gagcagccct cctacaattg gcgcgatttt tttcatccca gaactattta
     4141 cgtatgggcg ggccaatctg gccatcaaaa aaagtataat accgttaggc caaccccgtg
     4201 gaaaatttct tgcggcggct ctgttctttg catccaatag caaaatcctt gaacgacgca
     4261 cacttgaatt aaaccgggga gtaacatatg ctgattacaa tgaggccagg ttttcgcaaa
     4321 ttcgcactga cggcgcacgt cacaacttct gtcggttggt tcggagcagt gggtgcgttt
     4381 ctctgtcttg cgattaccgg cttgcgcacc acagacgcac atctgatgcg cgcaacatat
     4441 cttggtatgc aactggtagc tttttgcgta atcgtcccct tgagcttggc ctctcccctg
     4501 acgggacttg ttcagtcgct tggtacccac tggggtttgt ttcggcacta ttgggttcta
     4561 gtgaaattct taatcacggt tccatgtacc ataattttac tcctgcacat gcaggcgatt
     4621 cgcgctctcg cacaggccac cgcatcaacg acgttaaacg ctattgatgt cccggcgctt
     4681 caagttcaga tggttgtcaa cgcggccgta gcactcgtcg cattgctagt tgcgacggca
     4741 ctatcagtgt ataaaccacg cgggctgact ccttacggag cgcgttcaga aaacaggcaa
     4801 gaccgttccg ttttgcaatc cggcgacttc gcccgtcggc cgacctttac tcaaggtaaa
     4861 caatcattga gcccacgctg ggtttacgtc ctcggtttcg tgcttattgt catcgccgct
     4921 catattgtga tactgcacat ttccggtggt ggactaggcg ggcatactca ttgaaactca
     4981 cgctatttcc gggatattgt gaatgaacgc taaaggccca aaacgtggcc ctggccagcc
     5041 tccgatcagc ccaggcgatc ccctcaagtc aacaaatgtc ctgcttcccg gttcttatta
     5101 caaacgaatc gaaaaattag tggaagccgg aaaattcaaa actcaatcag aagcagtgcg
     5161 gcggatgatc gaagctggct tgaagaaatt tgaga
//
LOCUS       DAOQJL010000249         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9347_length_5186_cov_4.086728, whole genome shotgun sequence.
ACCESSION   DAOQJL010000249 DAOQJL010000000
VERSION     DAOQJL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9347_length_5186_cov_4.086728"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLG78_11350"
     CDS             complement(<1..235)
                     /locus_tag="PLG78_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23758.1"
                     /translation="MNIVSRILSVAFPFFVLLPFGAGLWLKKEPGSMDFAYFFYAYLA
                     ALLLAVYFAVRTTRDIALPLRALLSRIASYPVKQ"
     gene            complement(244..939)
                     /locus_tag="PLG78_11355"
     CDS             complement(244..939)
                     /locus_tag="PLG78_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE23759.1"
                     /translation="MEGPQNFKGMRILVVEDEDDIAQLIRFNLEEEGFLVTISGNGLD
                     ALSKLERNLPDAIILDLMLPGMGGVEILKKIRAKFDVPVVLVTARAGETDAVLGLEMG
                     ADDYVRKPFSPRELVARVKAVFRRKGTLRETATGSIERGGIEMDLSAHRVRVHGKEAD
                     LTLIEFKLLQLLMENSDMAFTRDRLLDRVWGREIYVSDRAVDVNIKRLRSKLGEEKER
                     LETVRGVGYRFRG"
     gene            complement(1012..1082)
                     /locus_tag="PLG78_11360"
     tRNA            complement(1012..1082)
                     /locus_tag="PLG78_11360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1048..1050),aa:Gly,seq:tcc)
     gene            complement(1164..1874)
                     /locus_tag="PLG78_11365"
     CDS             complement(1164..1874)
                     /locus_tag="PLG78_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNE23760.1"
                     /translation="MDFSIENKDGLTIVRFHVGNVSLFESRSLQSSIEKSTEDERVLA
                     LDLSSVEHMDSSALILFLKLSENLNLRGGFLILFGIRDSIKSLMTLTYLDHIVEIVAT
                     EADAIEIGTQTKNPREFVTSLNQALIFRGESRYHVIPYSEITHLESDGRATTVHTLKE
                     NYRTNKLLKDMETRIVSSIFLRIHKSYVVNMNYVNGMQYSVGGMYSIFLRDQANTSLP
                     VGRMYVQRIKALLGLGTD"
     gene            complement(2000..2767)
                     /locus_tag="PLG78_11370"
     CDS             complement(2000..2767)
                     /locus_tag="PLG78_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23761.1"
                     /translation="MKRILIVLFFTISHSLYAFGTYSEGWAVIVPTKIESSGIIFDSY
                     EGLFDVVTFDRNEKCVEKEDLCYSPKSKSIAFSVRPENAVVANFVQKNVGREMLVQYR
                     IHRFTAIALSSELEITDAKAIQTQLPADVKKQVLVKKSGSKRSFSVYGKILQFEYRGR
                     FIGTYEGIYYDKQRDKVHQFSVTNEEIAKQLQLCMQTNVPFYFGVTVSYVTAWRESDY
                     DIFEINYDGPAGQAKVEPTDDSKKDAKTEEKAPEPAK"
     gene            2837..4078
                     /locus_tag="PLG78_11375"
     CDS             2837..4078
                     /locus_tag="PLG78_11375"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNE23762.1"
                     /translation="MELNFQHADPGLSLMGEAAAARGLLESGKCKGSDFLGWLGLPSA
                     MVSQVSRIEEAAARIRKSDGLIVVGIGGSYLGARAVIEALGTDFPVVFAGHNMDSLYH
                     SDLLNSIQKKKYSINVISKSGTTTEPGLAFRILMNHMESRFSRDELAGLIFATTDANK
                     GSLRPLIKERGWTDFVIGDDVGGRFSVLSPVGLLPIAAAGKNIRRLLEGAQAMADIVR
                     KDSSEKNPALQYACYRNASYRSGKKIELFLSYQPGLHFIAEWWKQLYGESEGKEGKGI
                     FPASVDFSSDLHSMGQWIQEGERLIFETILDVIHPEKDIKIPSSPVDDGLEYLAGRSL
                     HEINRIALAAVQDAHSKGNVQSMKIMLDRMDEFNLGQLLYFYEYACAVSGIMLGVNPF
                     DQPGVEAYKKTMFRMLGKPGF"
     gene            4190..>5186
                     /locus_tag="PLG78_11380"
     CDS             4190..>5186
                     /locus_tag="PLG78_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE23763.1"
                     /translation="MNCPNCTKENAETAKFCKHCGTKLEITVEELGCTKCGKTLKAGA
                     KFCNHCGAPQHAPAVEKAPANREEDQKKAAEYMPGEAVGAMAELVNLIGLDAVKKTAD
                     KLRKLIKFNEERKKQGLPVGDISTHCVFLGNPGTGKTTVARILGKIFKDLGILPQGQF
                     IEVDKEALVAGYVGQTATKTKEIIDKAIGGILFIDEAYSLAGDSFGNEAIAQLLKRME
                     DQRGQFSVIVAGYDKEMKSFFDSNSGLRSRFQHYFQFEDYVPADLHRIATKLLKDKGY
                     QISPEGDQKLRDVLTTIYENRDAAFANGRTVRELIETSIMNLAARFDDGFSVEQLS"
BASE COUNT         1287 a         1204 c         1343 g         1352 t
ORIGIN      
        1 gttgcttgac cgggtagctt gcgatgcgcg aaaggagagc gcgaagtgga agtgcaatgt
       61 cgcgagtggt tctaactgcg aaatagactg caagcaacag ggctgccagg taagcgtaga
      121 agaagtaggc gaaatccata cttccaggtt ctttttttaa ccagaggcct gcgccaaagg
      181 gtagcagtac aaaaaacgga aacgcaacag ataggattct actgactatg ttcatgaaac
      241 cggttaaccc cggaatcgat atcctacgcc gcgcacagtt tccagtcttt ctttttcttc
      301 acccagtttg gatctgagtc tcttgatgtt tacgtccacg gcgcggtctg agacgtagat
      361 ttctcttccc cagacccggt ccagaaggcg atcgcgagtg aaagccatat ctgaattttc
      421 catcaacagt tgtaggagtt tgaattcgat caatgtgaga tctgcttctt tgccgtgcac
      481 gcgcactctg tgagcggata gatccatttc gattccgcct ctttcaatgc tgccagtagc
      541 ggtttcgcgg agagttcctt ttctccgaaa gactgctttg acgcgagcca ccagttctcg
      601 cggactgaac ggctttcgca catagtcgtc cgcacccatt tcgaggccca gcactgcgtc
      661 cgtttctccg gcacgggcag tgacgagcac gacgggcaca tcaaacttcg cacggatttt
      721 tttgaggatt tccacgccgc ccattccagg aagcattaga tcgagtatga ttgcgtctgg
      781 cagatttcgt tccagcttgc ttaaggcgtc caggccgttg cctgaaatag taaccaggaa
      841 tccttcctct tcgagattga atcgaatgag ttgtgcgatg tcgtcctcat cttctacgac
      901 gaggattcgc attcctttaa agttctgggg gccttccatg ttgttctacc agacagtaga
      961 aaacagattg gttacgtttt cattacaatc agttttctaa gtggtaaaac tagcgggcga
     1021 agggatttga accctctcga gaagcttgga aggctgccgt gctaccatta caccacgccc
     1081 gccggtgaat acaaaacgcg gctcgggggc cccccggtca atcggttttt tatcgagcag
     1141 ggcggccaca ggcgtgccag gaatcagtct gtgcccaggc caaggagtgc tttgatccgc
     1201 tgtacataca tccttccgac tggaagggaa gtgtttgcct gatcgcgcag gaagatgctg
     1261 tacattccgc ctacagagta ttgcatccca ttgacgtagt tcatattcac cacatagctt
     1321 ttgtgaattc taagaaagat cgatgagacg attctggttt ccatgtcttt gagaagctta
     1381 ttggttctat agttttcttt cagtgtgtgc acggttgtgg cccgcccgtc cgattccagg
     1441 tgagtgattt cggagtatgg aatgacgtga tatcttgatt caccccggaa gatcagagcc
     1501 tgattgagac tggttacaaa ttctcgcggg ttctttgtct gggtcccaat ttcgatggca
     1561 tctgcctctg tggccacgat ctccacaatg tgatccagat aggtgagggt catcagagac
     1621 ttgatggaat ctctgattcc aaatagaatc aagaacccgc cacgcaggtt gaggttttca
     1681 gatagtttca gaaacagtat gagtgcactc gagtccatat gctcgacaga agacaggtcc
     1741 agtgccagta ctctttcgtc ctctgttgat ttctcaatgg aactctgaag gcttctggat
     1801 tcgaacaggg aaacgtttcc aacatggaaa cgcacaatcg tcaagccgtc cttattttct
     1861 atggagaagt ccaaatcgcc aacccgcagg tgtatcgctc ttagatatga cgaacgggtt
     1921 gggaggcaag acttaaaaca agttagagcg gttgcaccgt tatgtgctgt tcattacctg
     1981 tttcatgtcg ttcattacat cattttgcag gttcgggcgc tttttcctca gtttttgcgt
     2041 ctttttttga atcatcggtt ggttctactt ttgcctgacc cgccggtccg tcatagttga
     2101 tctcgaagat atcatagtcc gattctctcc aggcagttac ataagaaacc gtcactccga
     2161 agtaaaacgg aacgtttgtt tgcatacata gttgcaattg tttagcaatt tcttcattgg
     2221 tcacggagaa ctgatgaact ttgtcgcgtt gcttgtcgta gtagatgcct tcatacgtcc
     2281 cgatgaaacg gcctctgtat tcaaattgaa gaatctttcc gtacacggag aagcttcgtt
     2341 tggatcctga tttctttacg agcacctgct tttttacgtc cgccgggagc tgcgtctgga
     2401 tggctttcgc atctgtgatt tccagttccg aagaaagtgc aatggcagtg aagcgatgaa
     2461 ttctatattg caccagcatt tcgcgaccca cgttcttttg aacaaagttg gccacgacgg
     2521 cgttctcagg ccttacgcta aatgcgatgg atttgctttt aggtgaatag caaaggtctt
     2581 ctttttcaac gcatttttcg tttctatcaa aggtaaccac gtcgaagagg ccttcgtagg
     2641 aatcaaaaat gattccagac gattctatct tcgtgggcac gatgaccgcc catccttcgg
     2701 agtaagtgcc gaaggcatac aggctgtgcg agatggtgaa aaaaagaacg attagaatgc
     2761 gtttcatagg cgttgacgaa tgaacccatt ggaagacagt gggattgttt ctgcaaggct
     2821 ttctcaggag taaaaaatgg aactaaattt tcagcatgct gatccaggac tgtctttgat
     2881 gggtgaggct gccgcagcac gcggtctgct tgaatccgga aaatgcaagg gatccgattt
     2941 cctgggctgg cttggacttc cgtccgcgat ggtttcacag gtttcgagaa tcgaagaggc
     3001 tgcggcccgg attcgcaaga gcgacggact cattgtggtt ggaatcggcg gatcttacct
     3061 gggagccaga gcagtcatcg aagccctggg aactgatttt ccggtcgttt tcgccggcca
     3121 taacatggac tcactctatc actccgacct gctgaattcg attcagaaaa aaaagtattc
     3181 gatcaacgta atttcaaaga gcggcacgac cactgagccg ggtctcgctt ttcgcattct
     3241 catgaatcat atggaaagca gattctccag agacgagctt gcgggtttga tttttgccac
     3301 aacggatgcg aacaaaggaa gtctgcgtcc tcttatcaag gaacgcggat ggactgattt
     3361 tgtaatcggc gacgatgtcg gtggaagatt cagtgtgctt tctcctgtag gccttctgcc
     3421 cattgctgcg gccggaaaga acatccgcag gcttctcgaa ggagcccagg caatggcgga
     3481 tatcgttcgc aaggacagtt cagaaaaaaa tccggcactc cagtatgcat gttaccgcaa
     3541 tgccagctat cgttcaggaa agaagataga gctgtttctg agctaccagc ccggtctcca
     3601 tttcatcgca gagtggtgga agcagctgta cggagaatca gaaggaaaag agggaaaagg
     3661 aatcttccct gcgtctgttg atttttcaag cgatctgcat tccatgggcc aatggattca
     3721 ggaaggggag cgtctgattt tcgaaacgat tctggatgtg attcatccgg agaaagacat
     3781 caagattcca tcttcgcctg tggacgacgg tcttgaatat ctggcgggtc gttctctgca
     3841 cgaaatcaac cgcatcgctc tggccgcggt gcaggatgct cacagcaaag ggaatgttca
     3901 gtccatgaag atcatgctcg atcgcatgga tgagttcaat ctgggccagc ttttgtattt
     3961 ttacgagtat gcctgcgctg tttccggaat catgctgggg gtaaacccgt ttgaccagcc
     4021 aggtgtagaa gcttacaaga aaaccatgtt tcgcatgctc ggaaagccgg ggttttagtt
     4081 ctgcggggcc ggtcgcgagc gaaaagtttc ctctttcttc ccggaaccat ttgacaggca
     4141 aaactcgccc cgcaagctct gtcgagccgc caggctttta ggatagtctg tgaattgccc
     4201 aaactgtacg aaagagaacg cggaaaccgc caaattctgt aaacactgcg ggaccaagct
     4261 tgaaattact gttgaggagt taggctgcac caagtgcggc aagacgctca aagcgggcgc
     4321 caaattttgt aatcattgcg gcgctccgca gcatgcgccg gcggtcgaaa aagctcctgc
     4381 aaatagggaa gaggatcaaa aaaaggcagc ggagtacatg ccaggggaag cggtaggggc
     4441 tatggccgag cttgtcaact tgatcggact tgatgccgtg aaaaagaccg cggacaaatt
     4501 acgtaaacta atcaagttca atgaggaaag aaagaaacaa ggcctgcctg tcggagatat
     4561 ttcaacgcat tgcgttttcc tcggaaaccc cggtaccgga aaaacaacgg ttgcacgcat
     4621 tttaggaaag atcttcaagg atttggggat tttgcctcaa ggccagttca tagaagtgga
     4681 taaagaggct ctcgtggcag gctatgtcgg acagacggct acgaagacaa aagagataat
     4741 cgataaggcc atcggcggca tccttttcat tgatgaggct tattccctgg caggcgactc
     4801 ctttggcaat gaggcaattg cacaactgct gaagcggatg gaggaccaac gcggtcagtt
     4861 ctctgtcatt gttgccggct atgataaaga aatgaagagc ttctttgatt ccaacagtgg
     4921 attgcgcagt cggtttcaac actactttca gtttgaagat tacgttccag ccgatttgca
     4981 caggattgcg accaaattgt tgaaggacaa aggatatcag atttctccag aaggggatca
     5041 aaaactcagg gatgtgctta ctacaattta tgaaaaccgc gatgcggcgt ttgctaacgg
     5101 gcgcacggtt cgcgagctga ttgagacatc aattatgaat ttggcggcac gtttcgatga
     5161 tggattctcc gttgaacaac tctcgc
//
LOCUS       DAOQJL010000250         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9363_length_5179_cov_3.862607, whole genome shotgun sequence.
ACCESSION   DAOQJL010000250 DAOQJL010000000
VERSION     DAOQJL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9363_length_5179_cov_3.862607"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLG78_11385"
     CDS             complement(<1..905)
                     /locus_tag="PLG78_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE23764.1"
                     /translation="MASEDAASGEVSAIPYLAGFKDNARNWTARDVVRADFKPVAGAI
                     LDEGFSDAAHWLKFSTPRTSGKQSMFLVFESLTLDRAELYSLENPESAVARAGDFYPR
                     SSWAVPWSDYPTFPVESGKSYLIRLQSTSLLMMPVTLRTESNLRGFTERRTFLSALYA
                     GFTLMLCLVAFGIFLALRDFVYLLYVGYVASQTLAFAALFSSFYRLLWPESSYIQNHL
                     YFFAQALSLAFGTSFFRAFTDLAERRPRLNILVTSVTVLSLIIAIGTLFSAQNVIFSR
                     SLTVIYLLWIPIFLFSILFGLSMDY"
     gene            complement(1077..2810)
                     /locus_tag="PLG78_11390"
     CDS             complement(1077..2810)
                     /locus_tag="PLG78_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23765.1"
                     /translation="MHLIQRFAFPFLVLFFSLIGSSHAQSESNFQPLEQHKFAEYELY
                     HYHAQLFGGWGPGNYPLIPMGVRIYFIPRTFDYPCCQSGVFLSAPRQAPHPSNYSIRM
                     DGFTGSPQGAEISLEAYEIGFSNDSESASDRFPNEKGMLYGGTWLTFTKIARVNPLLR
                     TPESDPNTHYLLRFQVQQPDFSIRLKQMPFQDFPNHPWPDASEFRDYGYTDEIGKSGM
                     VMLSGRLLLSPGLPRLASASPWTGPEVVNANTGIRTLRPAYALVAKNGWQSHYDLVAR
                     LSRGGSARDEYRSVKYMNFEPDANHTVIRFTGMIRYGTQAGDKPELLGEWMRKTTFSL
                     RKKPWGSLMASMNAYACVRPECEIKPMEVPSVQVFDTDAKENSDSLRINPDFRLGASR
                     YLNPNVGPYGDLNVPARLSFRLRSADGGALEGVSLEISASKADASIQIPKPAVTVGAM
                     FHKFLTLKYDPNFDPPMVGQIVPGFPARMEARQMVELDDAPGVRYKINCTVVIGSQRH
                     SCSRGSPGSVVTAVRQILPPEAGKTEGRARSFQRFEDFSSYTLGEHYTGADYDPVLDA
                     YPLFEIEVRKK"
     gene            complement(2965..>5179)
                     /locus_tag="PLG78_11395"
     CDS             complement(2965..>5179)
                     /locus_tag="PLG78_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE23766.1"
                     /translation="DVLILFNLPATMGIAIYPVGELQFIPAFLLAFVIVHYGVIPVIG
                     EATAIGNRVSLLILLFVPISILFYALGLRETASVTAILSHTIFVASPLALAFYMISFI
                     FLRPTALKMDETVRRLAEEKDRADAALHATEEARREIEALSRFTHIVNSHSDLEKIFK
                     EISQYVYQNFGISGSWLFLPDEKGEHLTSFRAYSYHEIRDDLYEFAKNLRIPLDSSGG
                     IAHVVWNRRKPLYLPQIRRFEFDFDRRLVENTGAQSFLHVPLVLNDRSVAMVIFTNVH
                     EPMRLSKKDIRTIETFCAQIAGVVNTVHLLRQTEKQKADMAALNRLTKSLNEDLDIET
                     ITRKLHEYVKENFGIQYYSLLCVDAAKTHLRLVAAALPDFVSAEDRDRIFHFHVPMKG
                     ARGGHGLTLRSKRPIYFKDFGEKDLRWLTDEERYVLDTCRIKSYLFLPLLLNGEPVGM
                     LNFSNSDGPLNLKDEDLTRFSILGEQMAGIITGSALFKEVQAARNSAESERKKSEKLL
                     LNILPADVASELKEKGATDPVLYENVSVMFTDFKGFTQIAETLSPRELIQDLDACFVQ
                     FDKVTERFNLEKLKTIGDSYMCAGGIPRRNKTHAIDCVLAALEIQAFMNLMKQIKTEQ
                     GLPYWELRLGIHSGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGTPGRINISGSTFE
                     LVKDVFDCEFRGYVSAKNKGEVAMYYVNGLKEEYSLAQDAKAPNGNFWKVYATVA"
BASE COUNT         1399 a         1322 c         1252 g         1206 t
ORIGIN      
        1 tggtagtcca tcgacagtcc gaacaggatg ctgaagagaa agattggaat ccagagcaaa
       61 tagatcacag tgagtgaacg ggagaatatc acattctggg ctgaaaacaa tgtgccgatt
      121 gctatgatga gagaaagaac agtcactgac gtaacaagaa tattgagcct cggcctgcgt
      181 tccgcaagat cagtgaaggc gcgaaagaaa gaagtaccaa acgcaagact cagagcctgc
      241 gcgaaaaaat acaggtgatt ctggatgtag gatgattccg gccagaggag cctgtaaaaa
      301 gaggaaaaga gtgccgcaaa cgcaagagtc tgcgatgcaa cgtatccaac atacagcaag
      361 tatacgaagt cgcgcaatgc gagaaaaatt ccaaaggcaa caagacagag catcaatgta
      421 aatccggcat aaagcgcgct taagaaagtg cgcctctctg taaagccacg caagttcgat
      481 tcagtgcgca gagtcacagg catcatcaga agagatgtgc tctgcaaacg aatcaaatag
      541 ctcttaccag attcaactgg aaatgttgga tagtcgctcc acggcacagc ccaggaagat
      601 cgtggataga aatcacctgc ccttgcaaca gcagactccg gattttccag ggaatagagt
      661 tccgcccgat ccagagtgag cgattcaaac acaagaaaca tgctctgctt gccacttgta
      721 cgaggtgtgg aaaattttag ccagtgagct gcgtcagaaa atccttcatc tagaattgcg
      781 cctgccacag gtttgaaatc tgcacgcact acgtctctgg ccgtccaatt tcgagcgttg
      841 tctttgaagc cggccaggta cggaatggca gagacttcac cgcttgcggc atcttctgaa
      901 gccacagaac aacccgaaga caacaaacac agcgtaaagc aacaaccaat ggcgttggtt
      961 cgaatccggg gcatgcggtg cagtcggcat gacgagtcca ggccgtaaac actgtttttg
     1021 aaacgatcgc ctaacgctat gatcagcggg ccttccgcga ggccccgcga gctgttctat
     1081 tttttgcgaa cttcaatctc gaagagcgga tacgcatcga gcacaggatc gtagtccgct
     1141 cctgtgtagt gctctcccag agtgtaagag ctaaagtcct cgaatcgctg gaaactgcgc
     1201 gcgcgtccct ccgtctttcc tgcctcggga ggcagaatct ggcgcactgc agtcacaacg
     1261 gatcctggac ttccacgact gcaggaatgt ctctgcgacc caatgaccac tgtgcagttt
     1321 atcttatagc gaacgccagg tgcatcatct aactccacca tctgtcgggc ttccattcgt
     1381 gcggggaatc ctggcacaat ctgacccacc atcggcggat caaaatttgg atcgtatttc
     1441 agggtaagaa atttatgaaa catcgcaccc actgtcactg caggctttgg gatttgaatg
     1501 gaagcatcgg ctttcgaggc actgatttct agagacacgc cttcgagggc cccaccatcc
     1561 gctgagcgca agcgaaagct caaccgggcc ggtacattta gatctccgta cggtccaacg
     1621 ttcggattca gatatctact tgctcccaga cgaaagtctg gattgatcct gagactgtcc
     1681 gaattctctt tcgcatctgt gtcaaagacc tgaacagacg gcacttccat tggcttaatt
     1741 tcgcattctg gacgaacgca cgcgtatgca ttcatagacg ccatcagaga accccacggt
     1801 tttttccgta gagagaatgt agtctttcgc atccactctc cgagcaactc tggtttatcg
     1861 cccgcctgag tgccgtagcg gatcatacct gtgaagcgaa taacggtatg gttcgcgtcc
     1921 ggttcgaaat tcatgtattt cacggagcga tactcatcgc gagcgctacc gccgcgcgaa
     1981 agacgggcca ccagatcata gtgactctgc catccattct tcgcgacgag cgcgtatgcg
     2041 ggacgtagtg ttcgaatgcc tgtattcgca ttcactactt ctggtccggt ccatggcgat
     2101 gcggaagcga gtcgaggtaa gccaggggac agaagaagcc gaccagagag catcaccatc
     2161 ccggattttc cgatctcgtc tgtgtaacca tagtcacgaa attcggaagc atctggccat
     2221 ggatgattcg gaaaatcctg aaaaggcatt tgtttcaagc gaatgctgaa atcaggttgt
     2281 tgcacctgga aacgaaggag gtagtgtgta tttggatccg attcaggcgt gcgcaacagt
     2341 ggattcactc gcgcgatctt tgtgaacgta agccatgtgc cgccatatag catgcctttc
     2401 tcgtttggaa agcgatcact cgcggactca gaatcgttcg agaaaccgat ctcataagct
     2461 tccagagaaa tttctgcacc ctgaggcgaa ccggtaaaac cgtccatgcg aatcgaatag
     2521 ttggatggat gcggagcctg gcgcggcgcg ctcaaaaaga ctccgctctg acaacagggg
     2581 tagtcaaaag ttcgcggtat aaagtagatt cgcacgccca tgggaataag cggataattt
     2641 ccagggcccc atcctccaaa taactgagca tgataatgat agagttcata ctctgcaaat
     2701 ttatgttgtt ccagtggctg gaaattgctt tcgctctgag cgtgcgaaga accaatcagg
     2761 ctaaaaaaca aaacaagaaa cggaaaagcg aaacgctgga ttagatgcat gcgcaaaagc
     2821 acatgccagg aggacacatc tcaagcagaa aacggattct aactcacgag aggatgagat
     2881 ggattcgcat tttaaaacag atcagataaa tgttcggggg aaattctccc ctatcgtttt
     2941 acttaaacaa gtagcctaac ctcatcaggc gaccgtcgcg tacaccttcc agaaattacc
     3001 gttaggcgct tttgcatcct gagccaggga gtattcctct ttgagcccat ttacgtagta
     3061 catggccact tcccctttgt tcttcgcact gacgtagccg cgaaactcac aatcaaaaac
     3121 atccttcaca agctcaaagg tcgaaccgct gatattgatc ctgcccggag tccccgagga
     3181 ttccatgcga gatgcggtat tgactgtatc tccccagaca tcatatgcga atttcttctc
     3241 tccgatcacg cccgcaacca gcgggccaga gtgaatcccc agcctcaact cccagtatgg
     3301 aaggccctgc tctgtcttga tctgcttcat cagattcatg aatgcctgaa tctcaagcgc
     3361 tgccagaaca caatcgatcg cgtgcgtttt atttctcctg ggaattccac cggcacacat
     3421 atagctgtct ccaatggttt tcagtttctc aagattgaat cgctctgtga ctttatcgaa
     3481 ctgaacaaag caagcatcca gatcctgaat gagctctcgc ggagagagtg tctccgcaat
     3541 ttgagtaaac cctttgaagt ctgtaaacat cacactcaca ttttcataca gtacaggatc
     3601 cgtggcaccc ttttccttca gctccgacgc cacatccgcc ggtagaatat tcaaaagaag
     3661 tttttccgat ttctttctct cagattcagc actgtttctg gccgcctgca cttctttgaa
     3721 aagagcagac cctgtgatga tccccgccat ctgttcgccc aggatggaaa agcgcgtaag
     3781 atcttcatct tttagattca gagggccatc ggaattcgag aaattaagca tccccacagg
     3841 ctctccattc aaaagcaaag gaagaaacag atacgacttg attctacaag tatcgagcac
     3901 gtacctctct tcgtctgtaa gccaccggag atctttctcc ccaaaatcct tgaaataaat
     3961 ggggcgtttg ctgcgaagag tcaacccgtg tcctccgcgg gctcctttca tgggcacatg
     4021 gaaatggaaa atccgatctc tgtcttctgc gcttacaaag tccggcaatg cagcggccac
     4081 gaggcgaaga tgagtcttcg cggcatctac gcacagcaga ctgtagtatt ggattccgaa
     4141 attctctttt acgtattcgt gcagtttcct ggtgatcgtt tcaatgtcca ggtcttcgtt
     4201 caagctcttc gtgaggcgat tgagcgccgc catgtccgcc ttctgctttt cagtctgtcg
     4261 aagtaaatga accgtgttga ccacgcccgc aatctgcgcg caaaaggttt cgattgtgcg
     4321 aatgtccttc ttggacagac gcattggttc atgcacgttg gtgaagatta ccatggcaac
     4381 gcttctatca ttgagaacga gcggaacatg tagaaaggat tgcgcgcctg tgttttccac
     4441 aagcctgcga tcaaagtcga actcaaacct gcgaatttga ggtaaatata gcggctttct
     4501 gcgattccat actacgtgcg caattccgcc cgatgaatcg agcgggatac ggaggttctt
     4561 tgcgaattcg tacaaatcat ctctgatttc gtgatagctg tacgcgcgaa agctagtcag
     4621 gtgctctcct ttctcatcag ggagaaaaag ccaggagccc gagattccga aattctgata
     4681 cacatactgc gatatttctt tgaatatttt ttccagatcc gagtgagagt tcacaatgtg
     4741 agtaaagcga ctgagcgcct cgatttcacg acgtgcctct tccgtagcgt gaagggctgc
     4801 atcggcacgg tctttttcct ccgccaggcg acgcactgtc tcatccatct tcaaagcggt
     4861 tggcctgaga aagataaagg aaatcatata gaacgcaaga gcaagaggag acgccacaaa
     4921 gatcgtgtgc gaaagaatcg cagtcacaga cgcagtttcg cgcagtccaa gagcgtaaaa
     4981 cagaatgcta atcggcacaa atagcagaat cagaagagag actcgatttc caatcgctgt
     5041 tgcttctcca attacaggaa tcactccgta gtggacgatc acgaacgcca gaagaaaggc
     5101 aggaatgaat tgcagctcgc ccacaggata gatggcgata cccatcgtgg ccggcagatt
     5161 gaacagaatg agaacatcg
//
LOCUS       DAOQJL010000251         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9377_length_5176_cov_4.273970, whole genome shotgun sequence.
ACCESSION   DAOQJL010000251 DAOQJL010000000
VERSION     DAOQJL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9377_length_5176_cov_4.273970"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLG78_11400"
     CDS             <1..1107
                     /locus_tag="PLG78_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE23767.1"
                     /translation="VMLTSAGVGALLIVSLYTVQATLQREMRFDGGGRPNLYIMDVQP
                     DQREGVEAVVHRYAASSQLVPMVSMRVKAINGEKIDRSNIEKNANRRNWENSLRSYEY
                     FASYRDHLNTSEKLARGSFWGTRRPENQEVSVDERWADRLGIRMGDLLEFDIGGAPFE
                     ATVTSMRNVDWAAMQINSILLFSPGAIEEAPHIFVSSLSVPAEKDRFRFQEELVNRYP
                     NISVIDVNQVVETIGGIAENVALVVRSIAFSTLIAGLIILTGAIGAGRFARVRESALF
                     KTIGARSRDIRVILSTEYALLGFLGALAGSVLSQAVSWPLLYYLFDLRAAVPLDAIVA
                     MSVSTGLISTLVGMLISRGVAASSPLEVLREETV"
     gene            complement(1104..1757)
                     /locus_tag="PLG78_11405"
     CDS             complement(1104..1757)
                     /locus_tag="PLG78_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23768.1"
                     /translation="MSFMLGMVHLIEPGTLVPDECSDRVARFLHSKEQNINAAPTQSN
                     SSRLQDMKLGQPMAPAADRASEEEDSCRKPLQTCGNCHLPTINNCNYQVVHYEKDKKV
                     ESLGQVLAQDDHTITIGRRACEGECVAETNTSRKVIQKEDIVKTVWLAGADAPEAWWN
                     FKKFSARGATAKKMLAIAQENNVSCANCHVAHGDFRLTSEGKEYLKTGKVIHTKPLH"
     gene            1969..3537
                     /locus_tag="PLG78_11410"
     CDS             1969..3537
                     /locus_tag="PLG78_11410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNE23769.1"
                     /translation="MQKHILAIDLGTSGPKVAIVSTTGQVMASEIEETQLILSHGGGA
                     EQDPADWWSAIARATRRLMESRHVPVESLAGIGVTAQWSGTVCVDAAGKHLSHAVIWM
                     DSRGADYVKKLTRGLIQIQGYGLSRLLKFIRLTGGAPGHSGKDSIAHILYIQATNPSL
                     YADTYKFLEPKDFLNLKLTGKFAATFDSIALHWLTDNRNINEIAYHDGLVSMAGIDRA
                     KLPDLKRSTDIIGVISPQAAAELGLPENLPVIGGTPDIQSAAIGSGAVRDFEAHLYIG
                     TSSWLTCHVPFKKTDLLHNMATLPSAIPGRYFIANEQETAGYCLTYLRDKIFYAQDSL
                     GIQPAPKDAYVRMTEAAASVPPGSDSLIFTPWLYGERTPIEDHSVRGAFYNQSLSTTR
                     AHMIRAVLEGVAFNSKWLLGAVESFMKRRAESINVIGGGARSALWCQILADVFDRTIR
                     QVHDPVQANSRGVGLLAAAALGLTTFEEISAQIPVSATYRPNSENRAVYDSLFKEFKN
                     IYKQNKSIYGRLNP"
     gene            3534..5066
                     /locus_tag="PLG78_11415"
     CDS             3534..5066
                     /locus_tag="PLG78_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE23770.1"
                     /translation="MIDPKTFAADHPALARFVEKTLLRFPVLDQWIRAIPSVRRQTEK
                     EYESLLGGMEESMKPYRKDFASYPELPKVGRKRSDILKDIQKMKAREESKWKSGYVSG
                     AVYHGDADHIDFLNKVYALQSQSNPLHFDLWPSINKYESEIISMTARMLGGTEDKSAD
                     QICGSMASGGTESILLAMKSYRDFARRERGIRRPELVVPVTAHAAFDKAAQYFKIKLV
                     RVPVGPDFRADVKLMEKAVTRNTIAMVGSAPTFPHGVIDPIRELSEIARKKGIGFHTD
                     ACLGGFVLPFARTLGYHVPPFDFRLPGVTSMSADTHKYGYAAKGTSVVLYRGEKLRSY
                     QYYTVTDWPGGLYFSPTFAGSRPGALIAAGWAAMLSMGEKGYLDATRKIMEAAHAIKS
                     EIRKIPDLYILGEPLWILAFASDTLDIYRVMEFMTHKGWNLNGLHKPACVHIAITLRH
                     TQPGVTKRFIKDLTDAVAHVRKHPEETGGMAPVYGMAATLPIRSVIGDLLKKYMDLLY
                     RV"
BASE COUNT         1187 a         1270 c         1359 g         1360 t
ORIGIN      
        1 gtcatgctta cctccgctgg cgttggcgca ctcttgattg tttctttata tacggtgcaa
       61 gcaacgctgc agagagagat gcgttttgac ggaggcggca gaccaaatct ctatatcatg
      121 gatgtacagc ctgatcagcg cgaaggagta gaggctgtgg ttcaccgtta tgcggcatcc
      181 tcacaactcg tccccatggt ttccatgcgc gtaaaggcga tcaacgggga gaaaatagac
      241 aggtccaaca ttgaaaaaaa tgcaaataga cgcaactggg agaacagtct tcgtagttac
      301 gaatactttg cttcttacag ggatcatttg aatacctcgg agaagctggc tcgcggaagc
      361 ttctggggaa ccaggcgccc ggagaatcaa gaagtgtctg tggatgagag atgggccgat
      421 cgactgggga ttcgcatggg ggacctgctt gaatttgaca ttgggggcgc cccctttgag
      481 gcaaccgtca cgagcatgag aaatgtagac tgggccgcca tgcagatcaa ttccatcctg
      541 ttgttttctc ccggggccat tgaagaagcg ccgcacattt tcgtttccag tctgtctgtt
      601 ccagctgaga aagacaggtt tcgttttcag gaagaacttg tgaatcgcta tccaaatatt
      661 tcagtgatag atgtgaatca ggtcgtagaa acgataggcg gcattgctga gaacgtggct
      721 cttgtggttc gctcgattgc attttccaca ttgatcgcag gtctgatcat tctgaccggt
      781 gccattggag ccgggcgatt tgcaagggtc agggagtccg ctttgttcaa gacgattgga
      841 gcgagaagtc gggacatccg tgtgatccta tctacagaat atgcccttct tggattcctg
      901 ggagcgctgg ccggatctgt tctttcccag gcggtttcct ggcccttgct ctactatttg
      961 ttcgatttgc gtgcggctgt tcccctggat gcgatcgttg cgatgagtgt ttctaccgga
     1021 ttgatttcaa ccctggtagg tatgctgatc agtcgcggcg tggcggcgag cagtccactt
     1081 gaagtgcttc gtgaggaaac agtttaatga agaggcttag tgtggatcac tttgccagtc
     1141 ttcagatatt cttttccctc tgatgtgagt ctgaagtctc catgtgccac gtggcagttc
     1201 gcgcacgata cattgttttc ctgcgcgatc gcgagcatct tttttgccgt agctccgcgc
     1261 gcagaaaatt ttttgaagtt ccaccacgct tcgggagcat ccgcacctgc aagccacacc
     1321 gtcttgacta tgtcttcttt ttgaatgacc ttgcgtgagg tattcgtttc tgccacgcat
     1381 tcgccttcgc acgctcttct accgatggtg atggtgtgat catcctgagc gagaacctgt
     1441 cccaaggatt ccactttctt atctttttcg taatgaacaa cttgatagtt gcaattgttt
     1501 atggttggca aatggcagtt tccacaggtt tgcagcggct ttctgcatga atcttcctct
     1561 tcgctcgccc ggtccgccgc cggcgccatt ggctgtccga gtttcatgtc ctgcaatctg
     1621 gaagagttgc tctgggttgg cgccgcattt atgttttgtt ctttggaatg gaggaatcgt
     1681 gccacgcgat cggaacattc gtctggaacc agtgtgcctg gctctataag atgaaccatt
     1741 cccagcatga aagacacttt ttgttcttca ttggtccatg gccgtgcctc tggatttcgt
     1801 ggccctttga gcccggaaag gcaggccatg caaaacaaaa tagttgataa gaatataaga
     1861 atgaacactc ggcgtttgat cacgcggatg agacgcacag gagagtgcgt tgggttctct
     1921 ttttttcgtt gcgggggaag gcggatcagt cgatttcgaa ccatccttat gcagaagcat
     1981 attcttgcga ttgatctggg aacctccggg ccaaaggtcg ccatcgtttc tactacgggt
     2041 caggtgatgg cctctgagat agaagagaca caactcattc tttcccatgg cggtggtgca
     2101 gagcaggatc ctgcagactg gtggtctgcg attgctcgtg cgacgcgccg gctcatggaa
     2161 tcgcgtcatg tgcctgttga atctctggcc gggattggag tcacagcgca atggtctggc
     2221 acagtttgcg tggacgcggc gggcaaacat ctttcgcatg ctgtgatctg gatggattcg
     2281 cgcggggcgg attatgtaaa gaagctaacg cgcggtttga ttcaaattca aggctacggg
     2341 ctttcgcgtc ttttgaaatt cattcgtctg acgggcggcg caccaggaca ctcaggaaaa
     2401 gactccatcg cgcatattct gtacatccag gctacaaatc cttctttgta tgcagacaca
     2461 tataagtttc ttgaaccaaa ggattttctg aatctaaaac tcaccggcaa gtttgcggcc
     2521 acgtttgatt ccatcgcctt acactggtta actgacaatc gcaatatcaa tgaaatcgcc
     2581 tatcatgatg ggctcgtctc aatggccggc attgatagag caaagcttcc agatctaaaa
     2641 cgatccacag atatcattgg cgtaatttct ccacaggcag ccgctgaact cggcctacct
     2701 gaaaaccttc cggtgattgg tggaacgcca gacattcaat ccgcggccat cgggtctggt
     2761 gctgtgcgcg atttcgaagc ccatctctac ataggaacaa gctcctggct tacctgccat
     2821 gtccctttca agaagacgga tcttcttcac aacatggcaa ctcttccttc cgcgattcca
     2881 gggcgctatt tcatcgcaaa tgaacaggaa actgccggct actgccttac ctacttgcgc
     2941 gataagattt tttacgctca ggattccctg ggaattcagc cggcccccaa agatgcatat
     3001 gtgcgtatga cggaggctgc cgcatctgtg cctcctggat cggacagcct catttttact
     3061 ccatggctgt acggtgagcg cacgccaatt gaagatcact ctgtgagagg agcgttttac
     3121 aatcaatcgc tgagcacgac tcgcgctcat atgatccgtg cagttctaga aggagtcgcg
     3181 tttaattcaa agtggctcct gggtgctgtg gaatctttca tgaagcgcag agcggagtct
     3241 atcaatgtga ttggtggtgg ggcgcgctcc gctctgtggt gccagattct cgcggatgta
     3301 tttgatcgaa ccattcgtca ggtgcatgac ccggtccagg cgaattcgcg cggagtgggg
     3361 ttacttgctg ctgcggctct tggattgact acctttgagg aaatttcggc acagattccc
     3421 gtttctgcaa cgtacagacc caactcggag aaccgggcag tctacgattc actgtttaaa
     3481 gaatttaaga atatttataa acaaaataaa tctatttacg ggcggctgaa cccatgatcg
     3541 atccaaagac tttcgctgct gaccatccgg ctctggctcg atttgtggag aaaacgctcc
     3601 ttcgttttcc tgtccttgat caatggatca gggccattcc atccgtgcgt cgtcagactg
     3661 agaaagaata cgaatctctc ctgggcggca tggaggaatc catgaagccg tatcgcaagg
     3721 acttcgcttc gtatcctgag ctgccaaaag tcgggcgcaa aagaagcgac attctcaaag
     3781 acattcagaa gatgaaagcg cgcgaagaat caaaatggaa gagcggatac gtgagcggag
     3841 ccgtgtatca cggggatgcg gatcacattg atttcttaaa caaagtgtac gctcttcaat
     3901 ctcaatccaa tccactgcat tttgatctct ggcccagtat caataagtac gaatccgaaa
     3961 tcatatccat gaccgcgcgc atgctcggcg gcacggagga taaatcggcg gatcaaattt
     4021 gcgggtccat ggcaagcggc ggaacggaaa gcatcttgct cgccatgaaa tcgtatagag
     4081 acttcgcgcg tcgcgagcgt ggtatcaggc gtccggaact tgtggttcct gtgacagcgc
     4141 atgctgcatt cgataaggcg gctcagtatt tcaaaatcaa gctcgtgcgt gttccggttg
     4201 gcccggactt tcgtgcagat gtgaagctca tggagaaagc agttacgcga aacacgattg
     4261 caatggttgg atccgcgcca acttttccgc atggagtcat tgatcccatt cgggaacttt
     4321 cagagatcgc gcgaaagaag ggaattgggt ttcacacgga tgcgtgtctg ggcggatttg
     4381 ttctgccttt cgcgcgcact ctcggatatc atgttccgcc gtttgacttt cgccttccag
     4441 gtgtgacttc catgtccgca gatacgcata agtatggtta tgcggccaaa ggaacgtctg
     4501 tcgttcttta taggggagaa aaactccgca gctatcagta ctatacggtg acagactggc
     4561 ccggcggatt gtatttttcc cccacattcg caggcagtcg tcccggtgca ttgattgccg
     4621 ctggctgggc cgcgatgctt tctatgggag aaaagggata tctggatgcg acgcgaaaga
     4681 tcatggaggc cgcccacgca attaaatcag agatcagaaa aatcccggac ctgtatatac
     4741 tgggagaacc actctggatt cttgcgtttg cctccgacac acttgatatt tatcgcgtca
     4801 tggagttcat gacgcacaaa ggctggaacc tcaatggcct tcacaaacca gcttgcgttc
     4861 acattgccat tactttgcgc catacgcagc ctggtgtaac caaacgattc atcaaggatc
     4921 taacagatgc agttgcgcac gtgcgaaagc accctgaaga aaccggcgga atggctcctg
     4981 tttacggaat ggccgcaacc ctcccgattc gcagtgtgat cggcgatctg ctaaagaagt
     5041 atatggattt gctataccga gtttgatttg cggattactt atccggtttt tctccgagct
     5101 cccaggtgaa tgctctggga actgcaatca acacggatct gaaatctatc ttctctccaa
     5161 tttttccaaa ttgcga
//
LOCUS       DAOQJL010000252         5157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9427_length_5157_cov_4.408075, whole genome shotgun sequence.
ACCESSION   DAOQJL010000252 DAOQJL010000000
VERSION     DAOQJL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9427_length_5157_cov_4.408075"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLG78_11420"
     CDS             <1..756
                     /locus_tag="PLG78_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23771.1"
                     /translation="VLAETPAGFHEDGIYLDLRGGQVWKRSGSVMRDLENGQKDSFTG
                     QEFGYTTFLRGNSDQIIANAAANSSAPDLSITSRNLGFGFEYGLFNYLGIGFSILDER
                     MNVRNIRSNISSDQTTLVALATAYPSASSAAYNQALINLEFLDPTVRVNRLPYLHATT
                     VNFQMRFHPLNGTLDPYAGFSLGIGRESQLPTNVLRPALHAGLRVLTRSVYTGIELEM
                     SFLEMGRKGTLPIPPHKQLTQTQFNIYAGLPLE"
     gene            complement(780..1982)
                     /locus_tag="PLG78_11425"
     CDS             complement(780..1982)
                     /locus_tag="PLG78_11425"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNE23772.1"
                     /translation="MNLSRLLSLIAFPILAAIASQCGGETGAIDQDRMQYFKSTYDES
                     RAEFRSLCKGLKGAVGSSLAVASTKESDLTIDTCSVGARVPQKLIIITSGLHGIEGFT
                     GSAVQRFWMKELLSSVDHERTGYLFIHAINPFGFRLERRVTENNVDLNRNFDVTPALF
                     SSHNDGYVKINELLNPDGKLNIGSMGNRFFPVRAVYNIIKHSMRALRQAALQGQYEFP
                     KGIYFGGSKFEQQKSLIEPLLVKESAPYSYVMVVDLHTGYGERGKLHFFPNAIKDATI
                     RKNIETVYAGYQVDYGDTADFYTTTGDFSEYVGKLLTAKKYIPMVFEYGTLDSQTTSG
                     SIKSIHNMIVENQGYWNGYESEGDLNEVKHRFREHFYPSSQVWRTKVIAISAEVFPLV
                     VDRFSKLQ"
     gene            complement(2013..3218)
                     /locus_tag="PLG78_11430"
     CDS             complement(2013..3218)
                     /locus_tag="PLG78_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNE23773.1"
                     /translation="MKLPLPAHEDLIVRRQADQLGIYVHFPYCIHKCSYCDFYSAGMD
                     RMPGGGKTVPVSDLESFAAQAQLEYQSRRDQFKKFGRVNSIFFGGGTASLLPAEYVDR
                     LLELFRSDFDTEGAEITVEGNPENFSPTYIESLARIGVNRIHVGVQSLHEHTLRDLDR
                     YYDRDRYSALIETLAHSRIGNRGVDLMYGVPNQTEGQFMADLRLLLSTGLTHVSLYSL
                     TAEPGTPYEAAVRKKKAPSPDEDLQAKLFQELPEFLSHEGFIHYEVSNFARPGFLCRH
                     NLRYWLYEPTLAIGPGAHGFDGSLRYANPRNVIAWQNSPATSPMTMHDPSLEIPLCIL
                     RILVPIAREDLESLFAPLDRGEQGMMLLQKWANQGLADFTGHFQWKSGAVLFLDDRIM
                     EMQEVLSPA"
     gene            complement(3215..4933)
                     /locus_tag="PLG78_11435"
     CDS             complement(3215..4933)
                     /locus_tag="PLG78_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNE23774.1"
                     /translation="MRHLIFLIAAAGVSCARLFSNPIDDLAKTAPAELVKQMTLEEKV
                     GQLIHIGMAGKAMNPALKADIEKYHVGGVILFEINLGKGPEIKALTDSIQDVESKSPN
                     AIPIFVSIDQEGGRVVRVKDGVEQFPGAAAIGQTGDEALAHDAGFITAQDLSALGIPF
                     VLAPVVDVNNNPANPVINTRSYGGDPNLVTRMALAYASGVMDASCIPALKHFPGHGNT
                     GTDSHTSLPTVKQDLAQMDAVELVPYKEGIKRGFPVIMSAHIVFPALDPAEPATLSPK
                     ILKDFLRTKLGFQGLITTDALEMKAVADRYPPPVLARKAFTAGVDVLLLTSSGERTRL
                     MFESLVDGFKKGELPMQDLDRAVERQIELKIRAGLFHKYKMNRPAWSTNVQEAFQVRE
                     EAQKKLRSEIAARYKDGLNRTISRASISSLRKAYNGVGQRADAKLFLRSPASVQQAQE
                     LGLEKQVTVSGFPVGSAAAVIAGFCRPGAQCPAAVIVELADFDLWRWNQLVAEMNDAR
                     KSNRTVPVLVGLYTGNPYLNVRVPSDGAVIGSFSPTEESLRALVYRAFEGGPVREARL
                     VLAEEK"
     gene            complement(4930..>5157)
                     /locus_tag="PLG78_11440"
     CDS             complement(4930..>5157)
                     /locus_tag="PLG78_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNE23775.1"
                     /translation="PVTLRNICPECRGGDTHCPVCNGRGSYKSGGMFRLNIDGGLVTG
                     QIIEIRLQGMRPAPLQYYKKSTIRIKVTRAA"
BASE COUNT         1259 a         1380 c         1251 g         1267 t
ORIGIN      
        1 gttctggcgg aaacgccggc gggttttcac gaagatggca tctacctgga cctgcggggc
       61 ggacaggtct ggaaacgatc cggcagcgta atgcgagacc tggaaaatgg ccagaaagac
      121 tctttcacgg gccaggaatt tggctatacc actttcctgc gagggaactc tgaccagatc
      181 atcgcaaacg cggctgctaa ctcaagcgca cccgaccttt ccattacttc ccgcaatctc
      241 ggattcggat tcgaatacgg actcttcaat tatcttggaa tcggtttttc gattctagac
      301 gagcgaatga atgtgcgcaa catcagaagc aatatctctt ctgaccagac cacacttgta
      361 gcactcgcca ctgcttatcc ttctgcgtct tcggctgcat acaaccaggc actcatcaac
      421 ctggaatttc tagatccaac cgtacgggtc aatcggcttc cttaccttca cgccaccact
      481 gtgaactttc agatgcgctt tcatccgctc aatggaacgc tcgatcctta cgcaggattt
      541 tcactcggaa tcggacgcga gtcgcagctt ccaaccaacg ttcttcgccc tgcattgcat
      601 gcgggactac gtgttctgac gcgatctgta tatacaggta tagaactgga gatgtccttt
      661 cttgaaatgg gcagaaaagg aacccttccc attccacccc acaaacaact gacgcagacc
      721 cagttcaaca tctatgccgg cctacccctg gaataagacg ttagacggca ttgtagcagt
      781 cactgcagtt tagaaaatcg atccaccaca agtggaaata cttctgcaga gatagcgatt
      841 acctttgtcc tccacacctg agaagaagga taaaaatgct cccgaaacct gtgcttcact
      901 tcgtttagat ccccttctga ttcatatcca ttccagtatc cctgattctc aacgatcatg
      961 ttgtgaatgg atttaataga tccagaggtg gtctgactgt cgagagttcc gtattcaaat
     1021 accataggaa tgtatttctt tgcggtgaga agcttgccca cgtactctga gaaatccccc
     1081 gtcgttgtat aaaagtccgc cgtgtctccg taatccacct gataccctgc atacacagtt
     1141 tcaatgtttt tgcgaatggt cgcgtctttg attgcattcg ggaagaagtg caacttgccc
     1201 ctttcgccat agcctgtatg caaatcaacg accatcacat aggaatacgg ggctgattct
     1261 ttcactaaca acggctcaat caatgacttc tgctgttcaa acttgcttcc accaaagtaa
     1321 attcctttcg ggaattcata ttgcccctgg agggccgcct ggcgcagggc ccgcatggaa
     1381 tgcttgatga tgttatatac cgcgcgaaca ggaaaaaacc gattgcccat gcttccaatg
     1441 tttaattttc cgtccggatt cagaagttca tttatcttta cataaccgtc gttatgcgaa
     1501 gaaaacaaag cgggagtcac gtcaaagttt cgattcaaat ctacgttgtt ttcagtgacg
     1561 cgcctctcca ggcgaaaacc aaacggattg atggcgtgaa taaataaata gcctgtccgt
     1621 tcgtgatcca cgctagagag gagttctttc atccagaaac gctgcacggc gctcccggtg
     1681 aacccttcaa ttccatgaag cccgcttgtg atgatgatca gcttctgagg gactcgcgcg
     1741 cccacgctac aggtgtctat agtgaggtca gattcttttg tagaagcaac cgcaaggctc
     1801 gagccaacgg ctcctttcaa acccttgcac agggagcgaa actctgcccg gctttcgtcg
     1861 tatgtagatt taaagtattg cattctatcc tggtctatcg cgccagtttc gccaccgcat
     1921 tggctggcta tagccgcgag aatggggaaa gcgatcaggg agagtagcct ggaaagattc
     1981 ataaccggaa ggtttcctgg tgcgcgaagt tgtcaagcag gcgaaaggac ttcctgcatt
     2041 tccataatgc gatcatccaa aaacagaaca gcgccggatt tccactgaaa gtggcctgtg
     2101 aaatcggcga gtccctggtt ggcccatttt tgaagcagca tcattccctg ctctccgcga
     2161 tcgagcggcg cgaacaaaga ctcgagatcc tctcgggcga tcggaaccag gattctcaaa
     2221 atgcagagtg ggatttcaag tgacgggtcg tgcattgtca taggagacgt ggccggagag
     2281 ttttgccagg caatcacgtt tcgcggattc gcgtaacgca agcttccgtc aaagccgtgt
     2341 gccccgggac caatcgcaag tgtgggctcg taaagccagt agcggaggtt gtgtctgcaa
     2401 agaaatcccg gccgtgcgaa atttgaaact tcatagtgaa tgaatccctc atgagaaaga
     2461 aactcgggca attcctggaa gagttttgcc tggagatcct catctggaga aggggccttc
     2521 tttttgcgca cggcggcctc atacggagtg cccggttccg cagtcagtga atacaatgaa
     2581 acgtgagtca ggccggtgga aagcagaagt ctgagatctg ccatgaactg cccttccgtt
     2641 tggtttggaa cgccatacat caggtccaca ccacggtttc caatgcgaga gtgagccaga
     2701 gtttcgatca atgccgaata acggtctcta tcatagtatc gatcgagatc tcgaagcgtg
     2761 tgctcatgga gagactgaac tcctacgtga atgcgattca ctccaatcct tgcaagactc
     2821 tcaatgtatg tgggagaaaa gttctccgga ttgccttcca ctgtgatttc tgcgccttct
     2881 gtgtcaaaat cactgcgaaa caattctaga aggcgatcaa catactctgc cgggagaaga
     2941 gaggcagtgc ctccgccaaa gaaaatcgaa ttcacgcggc cgaacttttt gaactgatca
     3001 cgtctggatt ggtactcgag ctgcgcttga gccgcgaaag attctagatc cgaaacaggc
     3061 actgtcttgc caccaccggg cattctgtcc atacctgcag aatagaaatc acaataagag
     3121 catttatgaa tgcagtatgg aaagtgaacg tagatgccca gctgatcggc ttgcctgcga
     3181 acgatcagat cctcatgcgc aggcaaaggc agtttcattt ctcttccgca agcaccagtc
     3241 ttgcttctct cacagggcca ccttcaaacg cgcgatacac aagtgcgcga agagattcct
     3301 ccgtaggaga aaatgaacca atcaccgcac cgtcagaagg aacacgaaca ttcaggtaag
     3361 gatttcctgt atacaaaccg accagaaccg gtaccgtgcg attcgacttg cgagcatcgt
     3421 tcatttccgc aacgagctga ttccatcgcc acaaatcaaa gtctgcgagc tccactatga
     3481 ctgcggccgg gcactgcgcc ccgggcctgc aaaaccctgc gatgactgcg gccgccgagc
     3541 caaccgggaa accggacact gtcacctgct tttcgagacc cagttcctgc gcctgctgca
     3601 cagaagcggg gctgcgaaga aacagcttcg cgtccgctct ctgtccgaca ccgttatacg
     3661 cttttctgag agaagaaatg gaggcgcgtg aaatcgttct attgagtccg tccttgtatc
     3721 tggctgcaat ttcagagcga agtttcttct gtgcttcctc tcttacctga aacgcttctt
     3781 gcacgttagt cgaccatgca ggccgattca ttttgtattt gtgaaaaagg cccgcgcgaa
     3841 tcttcagttc aatttgtctc tccacagcgc gatccagatc ttgcattggc agctcgccct
     3901 tcttaaatcc atccaccaga gattcgaaca tcaatcgcgt gcgctcaccg ctcgaagtaa
     3961 ggagcaacac atccacgccc gcagtgaatg cctttctggc gagcacagga ggaggatacc
     4021 gatccgccac agccttcatc tcaagagcgt ccgtagtgat gagaccctgg aagcccagct
     4081 tcgtacgaag aaaatctttt aagatctttg ggctgagagt ggccggctca gctggatcga
     4141 gtgccgggaa aacgatgtgg gcactcatga tcacaggaaa gccacgcttg attccttctt
     4201 tatatggaac cagttctacg gcgtccatct gcgccagatc ctgtttcacg gtcggcaaag
     4261 aagtatgact gtctgtgcca gtgtttccgt gacccggaaa atgcttcagc gcaggaatgc
     4321 acgacgcgtc cataaccccg gacgcgtagg caagcgccat gcgcgtaacc aggtttggat
     4381 caccgccgta cgatcttgta ttgatcactg gattggcagg attgttgttc acatctacaa
     4441 cgggtgccag aacaaacgga attccaagtg cgctcaaatc ttgagctgtg atgaagcctg
     4501 cgtcgtgcgc cagcgcttca tctccagtct ggccgattgc tgcggctcct ggaaactgtt
     4561 ccactccgtc tttcacgcgg acaacacggc cgccttcctg gtcaatgctt acgaagattg
     4621 gaatagcgtt aggcgacttt gattcgacgt cctgaataga atcagtcaat gctttgatct
     4681 ctggtccttt gcccagattg atttcaaaca gaatgactcc acccacatgg tatttttcaa
     4741 tgtccgcttt tagagcaggg ttcatggcct tccctgccat tccaatgtga atgagctggc
     4801 ctactttttc ctccagtgtc atctgcttga cgagttcggc aggcgccgtt ttcgccagat
     4861 cgtcgattgg attggagaat agtcgcgcac agctcacgcc ggctgctgcg atcaagaaga
     4921 ttaagtgtct catgctgctc tggttacctt gattcgaatt gttgatttct tataatactg
     4981 cagtggggcc gggcgcattc cttgaaggcg aatctctatg atctggccgg tcacaagtcc
     5041 accgtcaatg ttgagtcgga acattccgcc gctcttgtag gatccacgcc cattgcacac
     5101 ggggcaatga gtatctccgc ccctgcattc cggacatatg ttcctgagtg tcactgg
//
LOCUS       DAOQJL010000253         5147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9457_length_5147_cov_4.782797, whole genome shotgun sequence.
ACCESSION   DAOQJL010000253 DAOQJL010000000
VERSION     DAOQJL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9457_length_5147_cov_4.782797"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="PLG78_11445"
     CDS             <1..115
                     /locus_tag="PLG78_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23776.1"
                     /translation="DRARGFLVGQVMKETRGQAPAQEVNQLIDELVEKRRT"
     gene            complement(116..1075)
                     /locus_tag="PLG78_11450"
     CDS             complement(116..1075)
                     /locus_tag="PLG78_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE23777.1"
                     /translation="MHSDRESAARIVSEIRAADRRVRAKVSFLKYQDQIGLFLFVFAI
                     CGFFFTAWGALQGLSYWIVIPLIAFFTSILHEIEHDLIHDLYYRGNRWIQNLMFAFIW
                     ILRPNTISPWKRKGIHLLHHKVSGSPPDIEERLITNGMPYGFKRILCIMDGFLNMILR
                     RREVERIPGMRTFNIRLASLPMVPIFYGSILVECLLIATGNMSSIRTTLDFLMVVWVI
                     PNVIRQACLVFLSSTMHYYGGVDSVFRQTQVLNAWYFLPMQLFCFNFGSTHGIHHFVV
                     NQPFYLREMVKRSAHAAFRENGIHFNDLSTFRSANHYEPIGGI"
     gene            1160..2155
                     /locus_tag="PLG78_11455"
     CDS             1160..2155
                     /locus_tag="PLG78_11455"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNE23778.1"
                     /translation="MKTTVFGVGAWGTTLAGILAENGHDVVMWSHDPEIARTINEEHE
                     NKALFAGVKLSDKIRATTDLKEACVDSKIVVFVVASQFYASTVQKAHPFLPRDVVIVS
                     ATKGLNPADNRRPSEVLIENLPPEMKTRVAVLSGPNIAREIALKKPATTVIASPNEEI
                     AKAAQKFFSTPYFRVYTNTDMAASELGGTLKNVIAIAAGIVDGLDLGDNTKSALMVRG
                     LVEIIRFGTHFGAKADSFYGLAGMGDLITTCSSKMSRNHFVGESLAQGKTLKDILAGM
                     TAVAEGVEAARHVHEIAQREKIEMPVTTQVYKVLFEDKPVKQAIMDLMTRDLKAE"
     gene            complement(2165..2704)
                     /locus_tag="PLG78_11460"
     CDS             complement(2165..2704)
                     /locus_tag="PLG78_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNE23779.1"
                     /translation="MRVLIALLFLVPAGLFAHPGHAAGPLSGLWHPFTGLDHLLVMVA
                     VGFVASRLGGKVRLFAPAAFLLAMISGASASWAGVSLPGVEPLVQLSLAVFGVLLMAG
                     FRMHSGFFLVLVGVAGFFHGAAHASEGPAAISYAIGFLASTAALHVAGLLLGNIQTRA
                     GSVLSRAVGAAMIALAMAG"
     gene            2784..4601
                     /locus_tag="PLG78_11465"
     CDS             2784..4601
                     /locus_tag="PLG78_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNE23780.1"
                     /translation="MAALIQISGLHKQYGPRVLFDDAEVVISEDQKFGVIGRNGAGKS
                     TLFNIIPSKEEADSGSVWLSSDLRLSYLEQKDTHLPDETVIDFLMRTTDQEEWRCGKY
                     AGRFQIKGDMLGAKISGLAGGFRMRVKLTAMMLEEPNLLMLDEPTNYLDLKTLLMLEH
                     FLRTFRGAALIISHDREFLMKTCKHTLEVDNKTIASFPGNVEEYLEYKREVREQAIRE
                     NQNIEARRKELQNFVDRFRAKASKATQAQSKMKQISRLKTKEVAGAARSVRMRIPSPD
                     RQGGVALKCKDLRIGYGDRMIADNISFELKRGDRFAILGDNGQGKSTFLKTIQGSLPA
                     LAGEVRWGYDTKIGYYAQHVYETLGGEETVLQYLERYAPKSMTRQEILNMAGAFLFGG
                     DDTKKKVSILSGGERSRVLLLSLLLQKCDILLLDEPTNHLDFDTVEVLGEALQDFKGT
                     VFFVSHDRTFVNGVATGILEVKNKAIRLYEGSYEDYVYYLNEELDRAEASDDSEPVEQ
                     VETVQAAPPPGGKAKADKKARDKELKALEKKHATLGRELETLQQEQKDLLALVEKNPD
                     TVSKADYDRIGQLSSSVEEKEAEWLKVAESLERAKEPAG"
     gene            4598..>5147
                     /locus_tag="PLG78_11470"
     CDS             4598..>5147
                     /locus_tag="PLG78_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE23781.1"
                     /translation="MSLQVVNLKKTYKNPLSRKPPFEAVRGVSFEIKPGEIYALIGPN
                     GAGKSTIIKMIAGLIVPSSGDVMLDGKKLGSETAAYKKLSAVLEGTRNVYWRLTPLEN
                     LHYFANLRGISTTEIREKSVQLLKTLEIEGKKKNQSQHLSRGMLQKLALGAALITDPQ
                     IILLDEPTLGLDVGSSRKIKEMM"
BASE COUNT         1526 a         1284 c         1331 g         1006 t
ORIGIN      
        1 agatcgcgca cgcggtttcc tcgtaggtca ggtcatgaag gaaacccgcg gccaggctcc
       61 tgcgcaggaa gtcaatcagc tcatagacga gctggtagag aaacgcagaa cctgatcaaa
      121 tgccgcctat cggttcgtag tgattcgcgc tgcgaaaggt agatagatca ttgaaatgga
      181 ttccgttttc acggaaggct gcatgcgcgg agcgtttcac catctcgcgc aaatagaaag
      241 gctggttcac cacaaagtga tgaatgccgt gagtggatcc aaaattaaaa cagaacaatt
      301 gcatgggtag aaagtaccat gcattgagca cctgggtctg gcgaaataca gagtccaccc
      361 ctccgtagta gtgcatcgtg gaagaaagaa aaacaagaca ggcctggcga atcacgtttg
      421 gaatgaccca gactaccatc aagaagtcaa gagtcgttcg aatagatgac atgttcccgg
      481 tcgcaatcag gaggcattct acgagtatag atccgtaaaa aatcggcacc atggggaggc
      541 tggccaggcg aatattgaat gtcctcattc cagggatgcg ctccacttcc cgcctgcgaa
      601 gaatcatatt gagaaacccg tccatgatgc acagaatgcg tttgaaacca taaggcattc
      661 cgtttgtgat gagtctctct tctatatccg gtggacttcc agaaaccttg tgatgcagaa
      721 gatgaatgcc cttcctcttc cacggagaga ttgtgtttgg gcgaaggatc cagatgaaag
      781 cgaacatcag attttggatc cagcgatttc ctctataata aagatcgtgg atcagatcgt
      841 gctcgatttc atgaaggata gaagtaaaga aagcaatgag cggaatgacg atccagtaag
      901 acagaccttg cagggccccc catgcggtga aaaagaatcc gcagatcgca aacacaaaca
      961 gaaatagccc gatctggtcc tgatacttta gaaaactgac tttcgcacgg acccgccgat
     1021 ccgctgcccg gatttcgctt acaatgcgcg cggcactttc ccggtccgaa tgcatgatca
     1081 gagtatggat cggatacgaa aaagggtcca gcttttttct tcctcttgac cgcggatcgt
     1141 gtggagccaa gaatccgcca tgaagacaac tgtgtttggt gtgggtgcct ggggaacaac
     1201 tctggcggga attctggctg aaaacggaca cgatgtggtg atgtggtctc acgatccaga
     1261 gatcgcgcgc acgatcaacg aggaacatga aaacaaagct ctgtttgcag gcgtgaagct
     1321 atccgataaa atacgcgcca ccacggactt aaaagaggca tgcgtggata gcaagattgt
     1381 ggtatttgtg gttgcgtctc aattttacgc ctccactgtg cagaaggctc atccgtttct
     1441 tccccgcgat gtagtcatcg tttctgccac aaagggattg aatccggcag acaaccgcag
     1501 accatccgaa gtgctcatag agaatcttcc gcctgaaatg aaaacacgcg tcgcagtatt
     1561 gtccgggcca aacatcgcac gcgaaatcgc gctcaagaaa ccggccacca cagtcatcgc
     1621 atctcccaat gaagaaatcg caaaagctgc tcaaaaattc ttcagtactc cttactttcg
     1681 cgtgtacacg aacacagaca tggctgcatc tgaactgggc ggaactctca aaaacgtgat
     1741 cgccattgca gccggcattg tggatggtct ggacctcgga gacaatacga agtctgccct
     1801 gatggttcgc gggcttgtgg agatcatccg atttggaact cactttggcg cgaaggcaga
     1861 ttcattttac ggactcgcag gcatgggcga tctcatcaca acctgctctt ctaaaatgag
     1921 tcgcaatcat ttcgtaggcg aaagtctggc gcaaggaaag actctcaagg atatcctcgc
     1981 agggatgact gctgtggcag aaggtgtaga ggcggcgcga cacgttcatg aaatcgcaca
     2041 gagagaaaag atagaaatgc ctgtgaccac tcaggtatac aaggtactct ttgaagacaa
     2101 acctgtgaag caggccatca tggatttgat gacccgcgat ttgaaggccg aataaagtcg
     2161 gggcttagcc cgccatggcc aatgcgatca tggcggcacc gactgcgcgc gaaagaacac
     2221 tgcccgctct ggtctgaatg tttccaagca gcagccctgc cacatgcaag gcagctgtgg
     2281 acgctagaaa tccgatcgca taactgatcg cagccggacc ttccgatgca tgcgcggcgc
     2341 catgaaagaa tccggcaaca cccacgagca caaggaagaa accggagtgc atgcgaaagc
     2401 ccgccatcaa aagaacaccg aagactgcga gactcagctg taccagaggc tcaactcccg
     2461 gaagcgagac gcctgcccag gatgcagagg cacctgaaat cattgccagt agaaacgcag
     2521 cgggggcgaa gaggcgaact tttccgccca ggcgagaggc tacaaaacca accgcaacca
     2581 tcacaagcaa atgatccagg ccggtgaatg gatgccacag accagaaagt ggaccggctg
     2641 catgcccggg atgggcgaaa agaccggccg gaaccaggaa taaaagtgca atcagtacgc
     2701 gcatggaaaa cacaaaagag aaggggcgat ttcgaacaac taaaaagtaa tttatgcctg
     2761 ggggccaaaa aaaaacgtaa cggatggccg ctctcataca aatcagcggg ctccacaaac
     2821 aatatggacc cagagttctc ttcgacgacg cagaagtagt catctcagaa gatcaaaaat
     2881 tcggtgtaat cggacgcaat ggcgccggca aatcgactct cttcaatatc atcccctcca
     2941 aagaggaagc ggactccgga agtgtctggc tttcgagcga tcttcggctc agttatctgg
     3001 aacagaaaga cacgcatcta cctgatgaga cggtcatcga ctttctgatg cgcaccactg
     3061 accaggaaga atggagatgt ggaaagtacg ccggaagatt tcagatcaaa ggcgacatgc
     3121 tgggagcaaa gatctcgggt ctggcaggcg gctttcgcat gcgcgtaaaa ctgacagcga
     3181 tgatgctgga agaacccaac ctgttgatgc tcgatgaacc aaccaactac ctggacttga
     3241 aaactctact gatgctcgaa cattttctgc gaacgtttcg cggggccgct ctcatcatct
     3301 cgcatgaccg cgaattcctg atgaagacat gcaagcacac tctggaagtg gacaacaaaa
     3361 caatcgcaag cttccctgga aatgtggaag agtatctgga atacaaacga gaagtgcgcg
     3421 aacaggcaat cagagaaaac cagaacattg aagcacgcag aaaagaactc cagaacttcg
     3481 tagaccgatt ccgtgcgaaa gcatccaaag caacccaggc tcagtccaaa atgaagcaaa
     3541 tttcccgcct gaaaacaaag gaagtggcgg gagcggccag aagtgttcgc atgcgaattc
     3601 catcacctga tagacagggc ggcgttgcac tcaagtgcaa ggatctccgg attggatacg
     3661 gagacagaat gatcgcggac aacataagct ttgaattgaa acgcggagat cgctttgcga
     3721 tcctcgggga caacggccag ggtaagtcta cattcctcaa aacaattcaa ggatccctgc
     3781 ctgctctggc aggagaagtg cgctggggtt acgacacgaa gatcggctac tatgcgcagc
     3841 acgtgtatga aacgttaggc ggcgaagaga ctgttcttca gtatctcgaa cgttatgcgc
     3901 ccaaatccat gaccagacaa gaaatactga acatggcggg agctttcctt ttcggcggag
     3961 acgatacaaa aaagaaagta tccatcttat ccggtggcga aagatcgcgc gtgctcttgc
     4021 tttctcttct gcttcagaaa tgcgatatcc ttcttctgga tgaaccaacc aaccacctgg
     4081 attttgatac tgtggaagtg ctcggagaag ctctccagga tttcaaaggc acagtattct
     4141 tcgtcagcca cgacagaaca ttcgtcaacg gcgtggccac aggcattcta gaagtgaaga
     4201 acaaggcaat tcgtctctac gaaggctcgt atgaagacta cgtctactac ctcaacgaag
     4261 aactcgatcg cgcagaagca tccgatgact cagaaccggt agaacaagta gaaacggtgc
     4321 aagctgcgcc accgcccggt ggtaaggcga aagcagacaa gaaagctcga gacaaagaac
     4381 tcaaggcgct ggagaaaaaa cacgcaaccc tgggccgcga actcgaaact ctgcagcaag
     4441 aacaaaaaga tctgcttgcc ctggtcgaga aaaatccgga tactgtaagc aaagccgatt
     4501 acgaccgcat aggacaactt tcttcttctg tagaagagaa agaggctgag tggctcaagg
     4561 tcgcagaatc cctggaaagg gcaaaggagc cggccggatg agcctgcagg tagtaaacct
     4621 aaagaaaaca tacaaaaacc cactttccag aaaaccaccc tttgaagcag tccgcggagt
     4681 cagcttcgaa atcaaacccg gagaaatata cgcactcatc gggccaaacg gagccggcaa
     4741 atccacgatc atcaagatga tcgctggttt gattgtgcct tcttccgggg acgtgatgct
     4801 cgatggaaaa aagctgggaa gcgaaactgc cgcatataag aagctgagcg cagtcctcga
     4861 aggtacgcga aacgtgtact ggaggctcac tccactcgaa aacctgcact acttcgcgaa
     4921 cctgcgcgga atctcgacca cggaaattag agaaaagtca gttcagttac tcaagactct
     4981 agagattgaa ggcaaaaaga agaatcaaag ccagcacctt tcgcgcggaa tgcttcagaa
     5041 acttgctctg ggagcagcac tgatcactga tcctcagatc attctactcg atgaaccaac
     5101 tctgggcctg gatgtaggaa gctcgcgcaa gatcaaagaa atgatgc
//
LOCUS       DAOQJL010000254         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9483_length_5138_cov_4.559512, whole genome shotgun sequence.
ACCESSION   DAOQJL010000254 DAOQJL010000000
VERSION     DAOQJL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9483_length_5138_cov_4.559512"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLG78_11475"
     CDS             <1..279
                     /locus_tag="PLG78_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HNE23782.1"
                     /translation="QARQLLATEQVEVVFMEDGLDELAQFAHRVNEETENIGARRLHT
                     ILDRLLEDLSFRAPDLAADQRRVVIDRAFVLERLKPVMEDRDLSRYIL"
     gene            complement(286..987)
                     /locus_tag="PLG78_11480"
     CDS             complement(286..987)
                     /locus_tag="PLG78_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23783.1"
                     /translation="MIPPFQDHPLVEEFNELKKYFRQHETEASRRRIKQFRSFARVIA
                     EAGLPVGFDVMGSVNFGLATENSDVDLVLYVVCEDGYEGECPSPHCRTQQYVENLLIH
                     TWIRGISDSPYPVQVVDCINLNLLKRDLEKGDPESSCLLRFAFYRSVCRGVNARLLRP
                     YHKALLENQELVEKMKPYLWTLFDELVKSSKHNYSLMKYQDRLSQLGIGIPQSILGRI
                     RGHLDQSRRHHGDGI"
     gene            complement(1140..2030)
                     /locus_tag="PLG78_11485"
                     /pseudo
     CDS             complement(1140..2030)
                     /locus_tag="PLG78_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(2027..3142)
                     /locus_tag="PLG78_11490"
     CDS             complement(2027..3142)
                     /locus_tag="PLG78_11490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE23784.1"
                     /translation="MTSKLRVAVVHDWLTGMRGGEAVLESLLALYPHADLFTLLHNQG
                     SVSKFVEERKIRTSFIDRLPFKSRKYRNYLPFFPTAIETFSFRGYDLVISSSHCVAKG
                     VIVPPGTPHICFVHSPMRYVWDMTQDYFPYEFEDGSPGSLFNRFVFPFFANYLRMWDS
                     ASAHRVDRYIANSSFVAERIRKYYGRSAGVVHPPCIEEIPRSLPKERSDFYLIVSALV
                     PYKRVDLAIDAFRNLNRKLIIVGDGPDLKRLKSMAGPEIEFHGKVSREEIDRLYSQAR
                     ALVFPGIEDFGIVPVEAQAKGCPVIAYGRGGALETVTEKTGVFFHEQSPESLKAAVLE
                     CEKRRFLPENFRKNVGRFTNDIFLSKMSAEIKKLLRA"
     gene            3169..4008
                     /locus_tag="PLG78_11495"
     CDS             3169..4008
                     /locus_tag="PLG78_11495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE23785.1"
                     /translation="MAETTFHIRAQDGTRLAGSAWVPRRPKQVIAWIHGFAEHRKRYA
                     HLANWMNEERVALVAIDVRGHGESDGKRGYVHLFSDYFWDVSALLHYVRENFQKVPVS
                     IGCHSHGGLIVSRYLEENPPPVDLKCAVFSAPFLGLPPDFPPWKRKLGDAVAGLVPGL
                     SVPTGIDAKYISHDTKIQALYAGDPTIFKTATVRWFSEVLNAQAAALSRSPRIRLPAI
                     VLQGMADRIVSVDASRRFYEDLPSDKKKWVPYRGLYHEILNETNRLEVYKEIYKYIKK
                     FGR"
     gene            complement(4079..>5138)
                     /locus_tag="PLG78_11500"
     CDS             complement(4079..>5138)
                     /locus_tag="PLG78_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE23786.1"
                     /translation="GGGRQVITMDRYEANWSMVELGWKTHVKGEGRPFSSAQEAIATM
                     RKELPDAIDQDLPPFVIFENGKPVGPIKSGDVVIYFNFRGDRAIEISRAFTEKDFKPF
                     KRDPEVSVHYAGLMQYDGDLNLPEDYLVSPPAIDHTISQYLVDAGVKQYACSETQKFG
                     HVTYFWNGNNSQKFSETLEVWEEIPSDRISFDFKPEMKAKEITDAALKALSTKEFGFL
                     RVNYANGDMVGHTGNLPASIRAVEYVDEAIARLLEGAKAAGATVLITADHGNCDEMIM
                     LDKKGNAVKDEKGAYVPKTSHTLNPVPFILTGTASENYEIAAGKPGLANLAATVLTLL
                     GFQKPEMYLPSIVQLKSK"
BASE COUNT         1209 a         1246 c         1283 g         1400 t
ORIGIN      
        1 caggcccgcc agcttctggc aacagaacag gtggaagtag tatttatgga agatggcctc
       61 gatgagctgg ctcagtttgc gcatcgagtc aacgaagaaa cagaaaacat tggcgcgcgt
      121 cgcctgcaca cgattctgga tcgtcttctt gaggatttat cctttcgcgc cccggatctg
      181 gcggcggatc agaggagagt tgtgattgat cgtgcttttg ttcttgagag gctaaagcct
      241 gtgatggaag atcgtgatct ttctcgctac atcctttaaa cagggctaaa tgccatctcc
      301 atgatggcgt ctgctctggt ccaggtgccc gcggattctt ccgagtatgg attgtggaat
      361 tccgatcccg agctgagaga gtctatcctg atatttcata agcgaatagt tgtgtttcga
      421 gcttttgacc agctcgtcga ataacgtcca caaatatggc ttcatcttct ctaccagctc
      481 ctggttttcg agaagggctt tgtggtatgg tctgagcaat ctggcgttca ctccgcggca
      541 tacactgcga tagaatgcga atctcaaaag gcagctggat tcaggatctc ctttctccag
      601 atctctcttc agtaaattca gattaataca gtccactacc tgcaccgggt aaggcgaatc
      661 gctgattccg cgaatccacg tgtggatcag taaattttcc acatattgct gagtgcggca
      721 gtgaggagag gggcattcgc cttcgtatcc atcttcgcag actacgtaga gaaccagatc
      781 cacgtctgag ttctctgttg ccagaccaaa gttcacgctt cccatgacgt caaaacctac
      841 gggaagacct gcctctgcaa tcacgcgtgc gaaggagcga aactgtttga ttctgcgacg
      901 agaagcttct gtttcgtgct gcctgaaata cttcttgagt tcgttgaact cttctaccag
      961 agggtgatcc tgaaacggag gaatcattgt gcgattggcc tcccgcttgc gtcacgaacg
     1021 aatgcagtga ggtccaggtg agaaagctct ttggaatgcg gcatcatcac tgcgtggagt
     1081 ccattttcat ccacattgat gtccatcagc ggaatgcttt cttctccctt tagaatcatc
     1141 aaacgcaccc gatgcacgtt cttgttttca atgtatatct ccgcgtacgt tccacctggc
     1201 tggtatcctt caatggaatc cactccgcca aagttagacg gattcaggag tatcgttccg
     1261 ttctttttcg ctatgccgta atcttcgtgc acatgcccgg aaacggcaag aagcggggaa
     1321 tgatcgtcca ggtaattgcg cagtccctga gatcctacgt gccccatgga tgggatgcgg
     1381 tcaaaaaaac cgtaggccgg gttgtgaatc acacagatat ccgggacaat gtcatcaaag
     1441 aaattctccg gttcagaata gagtactccg tctatgattc gttcgtgaaa tacaacagag
     1501 agtttttccg gtattccaga tgtggccacg ggagctccgc cataccctgc aatcgtaagg
     1561 cgatcaaatt cacgcgtggc atgatggatg cttctggcac tcagtgcagt gtagcgcagg
     1621 tcaatgtcgt agttgcctgg taaaacgaga gtctccgcat tgctgtattt ttcaatgagg
     1681 ccttctatga gctcgtactt ctctttcatg gttttggccg cgatctgaaa caatcgtctg
     1741 tagtcggccg cctttctcac gaggtctgct tcatgatttg cagaatactt ttccgggaag
     1801 cgaagtatct cagtggcgag atcatacgga tacatcttct ccggcatttc tttgaggatt
     1861 ccgtagaact cgtcctggat acagacaaag tcgtatatat tgttttcgtc gtagaaggct
     1921 ttgtagagga tatcgccaga aagcaaatac agatccgctt ctgtattcga aagcaggatg
     1981 cgaagctctt tgagggcgtc gtgtatgtct gtggcgtaaa tgattttcat gcgcgaagga
     2041 gttttttgat ctctgcactc attttcgata ggaagatgtc atttgtaaac ctgccgacgt
     2101 tttttcggaa gttctccggt aaaaaacgcc gcttttcgca ttccagaacg gccgctttca
     2161 gtgattcagg ggattgctcg tggaagaaca cacctgtttt ttcagtgaca gtctcaagag
     2221 cacctccgcg tccgtaggcg atgacggggc agccctttgc ctgcgcttct accggcacaa
     2281 tgccaaagtc ctcgatgcct ggaaatacaa gtgctcgcgc ctgcgaatag aggcgatcaa
     2341 tttcttctct cgagactttg ccgtgaaact cgatttccgg acctgccatg gatttgagtc
     2401 tttttagatc cgggccgtct cctacaatga tcagcttcct gtttagattt cgaaatgcat
     2461 caatggccag atctactctt ttgtaaggaa cgagtgcact tacgatcaga tagaagtcgg
     2521 acctttcttt tggcagtgat cgcgggattt cttcaatgca tggcgggtga acgactccag
     2581 cagatcttcc gtagtactta cgaattcttt ctgcgacaaa gctgctgttt gcgatgtatc
     2641 tgtccacgcg atgcgcactt gctgaatccc acattctcag gtagttcgca aagaacggaa
     2701 acacaaatcg attgaacaga cttccaggag agccatcttc gaactcgtag gggaagtagt
     2761 cctgggtcat gtcccagacg tagcgcatgg gactgtgtac gaagcagatg tgaggagtcc
     2821 caggtggaac aatcactcct ttcgcaacgc agtgagagct ggatatgacc aggtcgtagc
     2881 cgcgaaagct aaacgtctca atggctgttg gaaaaaaagg cagataattg cgatactttc
     2941 tggatttgaa tgggaggcgg tctatgaaac tggtgcgaat ctttctttct tctacgaatt
     3001 tactgacaga accttggttg tgaagcagag taaaaagatc tgcgtgcgga taaagtgcaa
     3061 gcagcgattc gagcactgct tcccccccgc gcattcctgt cagccagtca tgtactactg
     3121 cgacgcgaag tttgcttgtc attcaggaag atgatttcat tcggacgtat ggcggaaacc
     3181 acatttcata tccgtgcgca ggatggcacc cggctggctg gcagcgcgtg ggtcccacgc
     3241 agacccaaac aggtgatcgc ctggatccat ggctttgcgg agcatcgcaa gcgatatgct
     3301 cacttggcca actggatgaa cgaagagcga gttgccctgg tggcgatcga cgtgcgcggg
     3361 catggggaat cagacggaaa gcgcggttat gtgcatctgt tttcagatta cttctgggac
     3421 gtatctgctc tactgcacta cgttcgagaa aactttcaaa aagttcctgt atccattgga
     3481 tgtcattcgc acggaggttt gattgtatcc aggtatttgg aggaaaaccc acctcctgtg
     3541 gatttgaagt gtgcagtgtt cagcgctccg ttccttggac ttcctcccga tttcccgccc
     3601 tggaaaagaa agctaggaga tgcagtggcc ggtcttgttc cagggctatc cgttccaacc
     3661 ggaattgatg ctaaatacat ttcgcatgat acaaagatcc aggctctcta tgcaggagat
     3721 ccaacgattt tcaaaacagc caccgttcga tggttcagtg aagtgttgaa tgcacaggca
     3781 gccgctcttt ctagatcacc gcgcattcgc ctgccggcca ttgtgcttca gggcatggct
     3841 gacaggattg tatctgtgga cgcatccaga cgcttctatg aagatcttcc ttctgataaa
     3901 aagaaatggg ttccctatcg cgggctctat cacgaaattc tgaacgaaac taatcgtctt
     3961 gaagtatata aggaaattta taaatatatt aaaaaatttg gaagatgaaa tcagggcaca
     4021 atccgaatgc caatgtgatt tgccaccggt ctttctgtgc ctggccagtg gtgctggatc
     4081 attttgattt taattggacg atggacggaa gatacatctc cggtttctga aatccaagca
     4141 gcgtgagtac tgtggccgcc agattcgcga gacccggttt tccagcagca atttcataat
     4201 tctctgacgc agtcccggtc agaataaagg gaaccggatt gagagtgtgg cttgtcttgg
     4261 gaacgtaagc gcctttttca tctttcacag cgtttccttt cttatccagc atgatcatct
     4321 catcgcagtt gccgtgatcc gccgtaatga gcacggttgc tcctgctgct tttgcacctt
     4381 cgagcagtcg tgcgatggcc tcatccacat actccactgc gcgaatgctg gcaggaagat
     4441 ttccagtgtg gcctaccata tctccatttg cgtagttcac tctcaagaat ccaaattctt
     4501 tcgtggagag agccttgagc gccgcgtccg tgatttcttt ggctttcatt tctggcttaa
     4561 aatcaaacga aattcgatcc gatgggattt cttcccagac ttcgagtgtc tcggaaaatt
     4621 tctgagaatt gtttccgttc cagaagtagg ttacatggcc aaatttctga gtttcagagc
     4681 aggcatactg cttcactccc gcatccacca ggtactgtga aatggtgtga tcgatggccg
     4741 gcggggaaac cagataatct tctggtagat ttagatcccc atcgtactgc atcaggccgg
     4801 cgtagtgtac agaaacttca ggatcgcgct tgaaaggctt gaaatctttt tctgtgaagg
     4861 cgcgcgaaat ttcaatggca cggtctccgc gaaagttgaa gtagatgacc acatctccgg
     4921 acttgattgg accaaccggc tttccgtttt caaaaattac aaacggtggt agatcctggt
     4981 caatggcatc cggcaattcc tttcgcatgg ttgcaattgc ttcctgggcg gagctgaatg
     5041 gtctgccttc tcctttgaca tgagtcttcc atccaagttc caccatgctc cagttcgctt
     5101 cgtatcgatc catggtgatc acctgacgtc cgcccccc
//
LOCUS       DAOQJL010000255         5120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9535_length_5120_cov_4.274235, whole genome shotgun sequence.
ACCESSION   DAOQJL010000255 DAOQJL010000000
VERSION     DAOQJL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9535_length_5120_cov_4.274235"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2506
                     /locus_tag="PLG78_11505"
     CDS             <1..2506
                     /locus_tag="PLG78_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNE23787.1"
                     /translation="SNSRTRLLAHQIEAAHTVVNALRPRFLIADEVGLGKTIEAGLII
                     KELMFRRGYHNILICVPAPLVVQWQSELRNRFNEEYTILQRSNYREVMEGKTRHMRII
                     TSIDFIKNPRYAESVLRKRWDLAVFDEAHRLRRDYNKVTVAYAFAEQVAEKCEALMLL
                     SATPFRGKLEELFYLIRLIDPHVLGPLSSFLQEEASGRTADLKRKLSQVLIRRRKVEV
                     GGFTKRHAQTIRFELSPEERAFYDETTEYVRREYNLALAEENRAVGFVMLVFQKLLDS
                     STRALMRALTNRKMMLERLVASSQTLPESPDESEWEDQEAPEELVGRVRDRRTWAELR
                     KEILTINRLLLLGRAIETDRKLIKTKETLQLLKSRGTNKFVIFTQFKSTVDYLCENLT
                     EWKVIPFHGSLRMAEKEQAIADFKDFADILICTEAGGEGRNLQFSNVLINYDLPWSPL
                     KIEQRIGRVHRFGQTKDVYIFNFATRDTVSERVLEVLTNKIKLFEESIGASDALLGAV
                     QDEVEFQRDLMEFITNKKTRKELNEELAAKLKTAESGYSKLHTLVSPSRMNFNWDDYY
                     GYTKRERSINSRTLEKITSNYLADVPQAGSLEKSGADYLLQRNGVRKQASFDSERALE
                     RDSLEFLALGHPLVEEAVDHYLSLPVGKSIHAIRDSRNGYYFIYSLRYSSNTMELTSV
                     FCSQDKPEEPLVLPDLILAPGHDAVAASFNADATENILLRRAAAAAAKAVEQDAETRR
                     LRMEETLKTVFRKEELKVESSHNKHLKGLREKQDIQKMRATMHPVFETRASLAKTEKE
                     ISRTQSARQIDLERIRRMSKIKAEIELLQIYRLMDR"
     gene            complement(2515..3270)
                     /locus_tag="PLG78_11510"
     CDS             complement(2515..3270)
                     /locus_tag="PLG78_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23788.1"
                     /translation="MNLFELSEEDFLRRLEDDMAITGDLGELFPRMVAYNVPYFVLSQ
                     YWPEERTNLLKDPLFPAFCVAEAGWKGRLENLKGSLDLSSGRLTCEKTYVMQCDVMYV
                     LVWAGEDLALCRVPRNASQTWTSRQEDTVRFVSKTGSVRDHFIVSVEQTLKREEYVTL
                     RKRNYVRAGMTIPRREMTGLAILAFGYLRFLKEDVSDLVGLRSELLQARATGELGAGA
                     VHAASQIFHQLLRVLPGDAHPFWGRAGSLFFQR"
     gene            complement(3267..4034)
                     /locus_tag="PLG78_11515"
     CDS             complement(3267..4034)
                     /locus_tag="PLG78_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23789.1"
                     /translation="MTTRKQGSRQSAGNRLLQATVLLCTLVTQNAYGRTGALYAELGF
                     VQGAYSGQGIRHQTQPALTEISQLTFLRASGERQSLLLLESAAAPDVRARGSGPRFAF
                     EYFLFPWLEIGRSFEPTVVRVHDVVAAQKLIWQYRAIAPAVGTFYSSGNLNGADFLGF
                     QRSTVKSQAYLGGGYEAAVYLPFWVVSPFARLSGDPFSGYASVAAGVKVFIEKFYISA
                     EGVASRQRVPLRNFNGSFTYRRAEETGFRICAGYRIH"
     gene            complement(3991..4302)
                     /locus_tag="PLG78_11520"
     CDS             complement(3991..4302)
                     /locus_tag="PLG78_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23790.1"
                     /translation="MEAKKVDHRRLSQVISQMKKDPEGSMGIYVVGGWRVQISRYKQT
                     SAERVRALYSRRRDQGLCIACGKKVTKTNPANGRLYRLCEDHRKNIDYKKAGKPAKRR
                     K"
     gene            complement(4315..>5120)
                     /locus_tag="PLG78_11525"
     CDS             complement(4315..>5120)
                     /locus_tag="PLG78_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE23791.1"
                     /translation="EALAQSRNVPAILVGRQVGLPRLSGQFRKFFFPDDSTFDRRFRM
                     DETIAIGSLEMSPLEMAVAFAAFADNGTIHRPYLILRIKDTSGKILYENDGSQDEFGT
                     HLPLMSRVLPGDVAEVMSSMLRDSAAFANTGVFSGLLMGKTGTTNEYRDAWFVGAIPG
                     LSASAWVGYDNPTYSMQGGMGARLAGPLFGRIVSRSGFSPNGSYAFSPSAERIRICKE
                     SGKISGPKCPSVTELFASVRRPEGICDLHDAGGNRTDRKDELSRQSDFE"
BASE COUNT         1414 a         1373 c         1202 g         1131 t
ORIGIN      
        1 ctccaactcc cgcacacgtt tgctggccca tcaaatagaa gcggcccata ccgtggtcaa
       61 tgctctgcgc ccgcgctttc tcatcgcaga tgaagtagga ctgggaaaga ccatcgaagc
      121 tgggctcatc atcaaggagt taatgttccg ccgcggatat cacaacatac tcatctgtgt
      181 tccggcaccc cttgtagtgc aatggcagtc tgagctcagg aaccgtttca atgaagaata
      241 cacaatcctt caacgctcca actatcgtga agtgatggaa gggaaaaccc gtcacatgcg
      301 aatcatcacg tcgattgatt tcataaaaaa tccacgttat gcggaatccg ttctcagaaa
      361 aagatgggat ctggcagtgt tcgatgaagc ccacagactg cgccgggact acaacaaagt
      421 tacggtggct tatgcctttg cagaacaggt tgcagaaaaa tgcgaagcat tgatgctact
      481 ttcagccact ccatttcgcg gcaaactgga agaactgttc tacctgattc ggctcatcga
      541 tccgcacgta ctcgggccgc tttcgtcctt tctccaggaa gaagcatccg gcagaaccgc
      601 agatctaaaa agaaaacttt ctcaagtatt gatcagacgc aggaaagtgg aggtgggagg
      661 attcacgaag cgacacgcgc agacgattcg cttcgagctt tccccagaag agagagcatt
      721 ctatgacgaa actacggaat atgtcaggcg agaatacaac ctggctctcg cagaagagaa
      781 ccgtgcagtc ggcttcgtta tgcttgtatt ccagaaactt ctggattcat ccactcgcgc
      841 actcatgcgc gcattgacca atcgaaagat gatgctcgaa aggctcgttg cttcttcgca
      901 aaccctgcct gaatccccgg atgaatccga atgggaagat caggaagcgc cggaagaact
      961 ggttgggcga gtgcgagaca gacggacatg ggccgagctt cgcaaagaaa tcctcacgat
     1021 caatcgtctc cttttgctgg gtcgcgcgat agaaactgac cgcaaactca tcaaaaccaa
     1081 ggaaacactt caacttctca aatcgcgggg cacaaacaaa ttcgtaatct tcacacagtt
     1141 taagtccact gtcgactatc tatgcgaaaa cctcacagag tggaaggtga tcccgtttca
     1201 cggctccctt agaatggcgg agaaggaaca ggcaatcgcg gatttcaaag atttcgctga
     1261 cattttgatt tgcaccgaag caggcggaga aggtcgtaac ctgcaattct caaatgtgct
     1321 catcaactac gatctgccct ggtctcctct caaaatcgaa cagaggattg gtcgcgttca
     1381 cagatttggt caaacaaaag acgtttacat tttcaatttt gcaactcgcg atacagtttc
     1441 tgagagagtt ctcgaagttc tgacaaacaa aatcaaactg tttgaagaaa gcattggcgc
     1501 ctctgatgcg ctcctcggag cagtgcagga cgaagtggag ttccagagag atctgatgga
     1561 attcatcaca aacaagaaaa cgcgcaaaga actcaatgaa gaactcgcag ccaaactaaa
     1621 gactgcagag agcggatatt ccaagcttca cactctcgtc tctccttcca gaatgaactt
     1681 caactgggac gattattacg gttacacgaa aagagaaaga agcatcaact caagaactct
     1741 ggaaaagatc acttccaact atctcgcaga cgtacctcag gcggggtctc tcgagaaatc
     1801 gggcgcagac tatcttctgc aaaggaacgg agtgcgcaaa caggcttcct ttgattcgga
     1861 acgagcgctg gaaagagact cgctcgaatt cctggcactg ggccatcctc tagtagagga
     1921 ggcagtggat cattatctat ctctccctgt tggtaagagc atccacgcca ttcgagattc
     1981 acgcaatggt tactatttca tctactcgtt gcgttactcc tccaatacaa tggaactgac
     2041 gagtgtgttt tgttcacagg ataagccaga agagccgctc gttttgccgg atttgatcct
     2101 cgctccgggc cacgacgcag tggcggcttc attcaacgcg gacgcaacgg aaaacattct
     2161 actcagaaga gctgctgcag ccgcagcaaa agcagtcgaa caggatgcag aaacgagaag
     2221 actgcgcatg gaagaaacat tgaaaacagt cttccgcaaa gaagaactca aggtggagtc
     2281 aagtcacaac aagcatctca aaggcctcag agaaaagcag gacatccaga agatgcgcgc
     2341 caccatgcat cctgtttttg aaacaagagc aagcctggcc aaaacagaaa aagaaatctc
     2401 gcgcacacaa tctgcgaggc aaatcgatct ggaacgaatt cgtcgaatga gcaaaataaa
     2461 agcagaaatt gaacttctgc aaatctatag actcatggat cgttgaagaa agttctatct
     2521 ctgaaagaaa agagaaccgg cgcgacccca gaatggatga gcgtccccgg gtaatacccg
     2581 caacaactga tggaatatct ggctggcagc gtgcacggcc ccagcaccaa gctcgcctgt
     2641 ggcgcgtgcc tgcaaaagct cagagcgaag cccaaccagg tcagatacat cctctttcaa
     2701 aaatcgcaaa tatccaaacg cgaggatggc cagtcccgtc atttcccgac gaggaatggt
     2761 cataccggct cgcacataat ttcgctttcg cagcgttaca tattcctctc gcttcagagt
     2821 ctgctccaca ctcacgatga aatgatcgcg cacgctacct gttttcgaaa caaatcgcac
     2881 agtgtcttcc tgccgactgg tccacgtctg cgaagcattg cgcggaactc tgcaaagagc
     2941 cagatcttcg ccggcccaca ctaaaacgta catcacatcg cactgcatca cataggtctt
     3001 ttcacacgtt aggcgaccag acgacagatc gagcgatcct ttcagatttt caagcctgcc
     3061 tttccacccc gcctctgcga cgcagaaggc gggaaacaga ggatccttga gtaagtttgt
     3121 cctttcttcg ggccaatact gggatagaac aaaataggga acgttatacg caaccatacg
     3181 aggaaagagc tcacccagat cccccgtgat cgccatgtcg tcttcgagac gacgcagaaa
     3241 gtcctcttca cttagttcaa atagattcaa tgaattctat accccgcgca aattctaaag
     3301 cctgtttctt ctgcccttcg atacgtgaat gatccattga aattccggag tggaactctc
     3361 tgacgagagg caactccctc tgcggaaata tagaatttct caataaagac cttcacacct
     3421 gccgccacac tcgcatatcc agaaaacgga tcaccggaca gtcgggcaaa aggcgatacc
     3481 acccagaaag gcagatagac ggcggcctca taaccgccgc ccaggtaggc ctgggacttg
     3541 actgtggacc tctgaaatcc caggaaatca gcgccattga gattgcccga actatagaac
     3601 gtaccgaccg caggtgctat ggcccggtac tgccagatca atttctgcgc ggcgactaca
     3661 tcgtgaaccc ggacaacggt gggctcaaaa ctccgcccga tttccagcca tggaaagaga
     3721 aaatattcaa aagcaaagcg aggccccgac ccgcgtgcgc gtacatccgg cgcagccgcg
     3781 ctttccagga gcaagagcga ctgacgttcc ccagaagctc gtaaaaaggt cagctgcgaa
     3841 atctcggtca aggcgggctg ggtctggtgg cgaattccct gacctgagta ggctccctga
     3901 acgaacccca gttccgcata caatgcaccg gtcctgccat atgcattctg tgttaccaga
     3961 gtgcacagta ggaccgttgc ttgcagtaac ctatttccgg cgctttgccg gcttccctgc
     4021 tttcttgtag tcaatattct tgcgatgatc ttcgcagagg cggtacagtc gaccattagc
     4081 cggattggtt ttggtaactt ttttgccaca ggcaatgcaa agtccctgat ctcttctgcg
     4141 gctgtacaga gcacgcaccc gttcagcgct ggtttgtttg taacggctga tttgaacgcg
     4201 ccatccaccg acgacataga tccccatgct gccttctgga tctttcttca tttgagaaat
     4261 cacctgcgag agacgacggt gatcaacttt ttttgcttcc atgtttgtgg tcctttattc
     4321 gaaatccgac tgacggctca gttcatcctt acgatcggtg cggttaccgc ctgcatcgtg
     4381 caggtcgcat atcccttctg gtctacgaac agacgcaaac aattcggtca cggacgggca
     4441 cttcggtccg gaaatttttc cagactcttt gcaaatacga attcgctccg cagatggaga
     4501 gaacgcatag ctgccattag gcgaaaaccc tgaccgtgaa acgattcgtc cgaacaaagg
     4561 gccagccagg cgcgcaccca ttccaccttg catcgagtag gttggattat catatccaac
     4621 ccatgcggat gcagacaaac caggaatcgc gccgacaaac catgcatctc tgtattcgtt
     4681 cgtcgttcca gtttttccca tgagcaaccc ggagaataca ccagtgtttg cgaatgcagc
     4741 ggaatcccgc aacatactcg acatcacttc agctacatct ccaggaagaa cacgagacat
     4801 taatggtaga tgtgtaccaa actcgtcctg cgatccatca ttttcataaa gtatttttcc
     4861 tgatgtgtct ttgattctca gtatcaagta gggcctgtga atcgttccgt tatccgcgaa
     4921 cgccgcgaag gcgaccgcca tttcaagagg agacatctcc agagaaccaa tcgcaatcgt
     4981 ttcatccata cgaaatcgtc tgtcaaaggt actgtcgtct ggaaaaaaga attttcgaaa
     5041 ctgtccggac aaacggggca atcccacctg acggcccacg agtatagcgg ggacgttacg
     5101 cgattgagcc agtgcttcac
//
LOCUS       DAOQJL010000256         5119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9540_length_5119_cov_4.429305, whole genome shotgun sequence.
ACCESSION   DAOQJL010000256 DAOQJL010000000
VERSION     DAOQJL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9540_length_5119_cov_4.429305"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLG78_11530"
     CDS             complement(<1..577)
                     /locus_tag="PLG78_11530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE23792.1"
                     /translation="MALRVKLGLLELALNRFASDFLRLISICAVLVIHSTGEFERSFA
                     SSHRFFSAEFVGIVLNQLARFSVPVFIMLSGFALSLRYKGDLDVREFFVRRASKIGIP
                     FLAWTPVFLLAHQIGQHPDLMVSASGWPDALVSALGELPAALFTYGADYHFYFFIIIL
                     QCYLLFPLLLRASGKVWWILIPLHLITHAPAH"
     gene            complement(590..1426)
                     /locus_tag="PLG78_11535"
     CDS             complement(590..1426)
                     /locus_tag="PLG78_11535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNE23793.1"
                     /translation="MSEDLSVFEWRRKLHEALHGVGIEAADLEADLILRDILNLDAAG
                     LILNKNNLVPADKTVRLTAVMHERLKRRPLAHVLGYTEFYGRRFRTDARALVPRKETE
                     LLVEQLLLAPLPAGPVLDLACGTGIAGITIALEKPGTIVHCSDLSEDALSLARENALA
                     LKAKVEFYMSDWFESVPDVRYAAILCNPPYIWLRDSNSLEPEVLFDPAMALFHENPPA
                     LYEHLLGEAKMRLVRGGFVFFELGADIAHAVLERAQKIFVNARILADYSGLPRMLIAS
                     TE"
     gene            complement(1423..2304)
                     /locus_tag="PLG78_11540"
     CDS             complement(1423..2304)
                     /locus_tag="PLG78_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNE23794.1"
                     /translation="MKDQIRRGIVDLGLSDAARASGVAVFPFASGLFELFRVDLPSTS
                     RAPARSLIVKCVSSLDMAEAEWDGLVRLREAGAVVPELIGFVKTDSGGRSGALLFLSM
                     VSGGRGSVRLESLIQIYSTRGESYGYSRDNYIGSLHQRNRRYATFEEFWAQDRIFPQL
                     NMVISAGLLTQKDLENARRVLDIASGWELSQPGPRLIHGDLWNGNLLIDSDMNPVLID
                     PSVSYSHPEQDLAMLELFGSPLSSSDLDLLARETGLPAGRIERRDFFQLYPLLVHVNL
                     FGASYVSQARRAIGRYL"
     gene            complement(2304..2888)
                     /locus_tag="PLG78_11545"
     CDS             complement(2304..2888)
                     /locus_tag="PLG78_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23795.1"
                     /translation="MDPFTANKYYAKKKLLKLFGGEVRIYDESRTNLLFFVKQKAFKL
                     KEDITVFADESQSRPLLKIQARSILDFGTTYDVTDVVTNQKVGAIRRKLLKSILRDEW
                     AILDASDKEFGTVSEDSMLMALLRRFLSNLIPQHFAMTVGGTQVGDLKQTWNPFVPQF
                     NVDFSMDSGRLDRRLGIAAVVLLQVIEGKQSSMD"
     gene            complement(2892..3692)
                     /locus_tag="PLG78_11550"
     CDS             complement(2892..3692)
                     /locus_tag="PLG78_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE23796.1"
                     /translation="MEISTVLGLVGGFTVVLLTLLIEGGHVASFINFGALILILGGSV
                     AVAVVSFSLKEVTAIPQYFMKAIFPPKENLKEMVELFVNLSERARREGLLSLEDDLSS
                     FKDKMMRTGMELVVDGTDPEIVRDILEDYADAEKEHDKIPPELMETLGGFSPTLGIIG
                     TVMGLVHVLEGLGGGGGIEQLGRGIAVAFIATFYGIGFANLLWLPMANKLKFLSHRSV
                     THRNIIIKGVMSIQSGDHPRVIREKLLCQISDPHLRQEIEEAAPKEGA"
     gene            complement(3706..4440)
                     /locus_tag="PLG78_11555"
     CDS             complement(3706..4440)
                     /locus_tag="PLG78_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNE23797.1"
                     /translation="MARKNKAPEQHKASGESGSGRWMLTYLDMVTLLFGVFVILYAMS
                     QVDTNKMKAVAESLRMGFQGGATIDEGSLSGGRTIIEDLFPEGVRARRVRARITGLFR
                     QEMLQKLIKVTEDERGVVVSLIGMDNFRPGSSELTEETQQLLEKMSLLLAEVEYPVRI
                     EGHTDDEVQDNNGAGISASDWELGAKRSVNVILFLETRDVDPRKMSAVSFGSTRPLDR
                     EGTPESHALNRRIDIIIVTGEKLKVK"
     gene            4611..>5119
                     /locus_tag="PLG78_11560"
     CDS             4611..>5119
                     /locus_tag="PLG78_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23798.1"
                     /translation="MRRIVWTLVLFTATVSANSSEKRALLLPVQAIFIHESMPARPEA
                     ALGLQIERGASMMIQSRGLRLTGASSATITKPEPGLVQSLAAKHGVDLVYWVEATQKE
                     LNYPRENSAQTAFCDDSEKDEPIRGSLSIKVRIFDAEGKEVWTASSTNDYRCSFPVTE
                     AWDQTMRLL"
BASE COUNT         1386 a         1359 c         1229 g         1145 t
ORIGIN      
        1 gatgcgccgg tgcatgcgtg atcaagtgaa ggggaatcag gatccaccac actttcccgg
       61 acgcacgcaa aagcagaggg aaaagcagat aacactgaag aataataata aagaaataga
      121 aatgatagtc cgcgccatac gtgaataacg ccgccggtag ttctcccagt gcggacacca
      181 gagcatccgg ccaaccggat gcggacacca tcaggtctgg atgctggcca atctgatgtg
      241 cgaggagaaa cactggagtc cacgctaaaa atggaattcc aatcttcgaa gcgcggcgta
      301 caaagaactc ccgcacatcc agatcccctt tgtagcgcaa cgacagagca aacccggaca
      361 gcatgatgaa aacaggaact gaaaagcgtg ccagttgatt gagaacaatc cctacaaact
      421 ctgcagaaaa aaagcgatgg ctcgaagcga aggatcgctc aaactctccc gtgctatgaa
      481 ttacaaggac ggcgcaaatg gaaatcaggc gaaggaagtc ggacgcaaaa cgattcaaag
      541 ctaactccag caggcccagc ttgacgcgaa gagccacaca ctcaatccat tactcagtgc
      601 ttgcaatcaa catacggggc aacccggaat aatccgcaag aattctggca tttacgaaga
      661 tcttctgtgc gcgttctagc acagcgtgcg caatgtcagc gccgagctca aaaaagacga
      721 acccgcctct gaccagacgc attttcgcct ctccaagcag atgctcgtac agtgcgggag
      781 gattctcgtg aaagagagcc atcgccggat caaataatac ctccggttcg agagagttac
      841 tgtcacgtaa ccagatgtac ggtggattgc ataggatggc cgcataacgt acgtctggga
      901 cggattcaaa ccagtcgctc atatagaatt caactttcgc tttcagtgca agtgcattct
      961 cacgggccag ggacagggca tcctctgata ggtcactgca gtgcacaata gtaccaggct
     1021 tttcaagcgc aatcgtaatg cctgcaatgc ccgtcccgca cgcaagatcg agaactggtc
     1081 cggcgggcag aggagcgagt aacaattgtt ctacgaggag ctctgtttct tttcgcggga
     1141 ccagggcacg tgcgtccgtg cgaaatcggc gaccgtaaaa ctcagtatat ccaagaacat
     1201 gagcgagcgg tcgacgcttg agtctttcgt gcattactgc tgtaagtcga actgtcttgt
     1261 cggcgggaac caggttgttc ttgttcaaga tcaaaccggc tgcgtcgaga ttcaggatgt
     1321 ctctaagaat caaatctgcc tcgagatcag ccgcttcaat acccacacca tggagggctt
     1381 cgtgcaattt tcggcgccat tcgaatacgc tcagatcttc gctcacagat accgtccgat
     1441 tgcgcgacga gcctgcgaaa cgtaagacgc gccaaacaga ttcacgtgaa cgagaagtgg
     1501 atagagctga aagaaatcgc gcctctctat tctgccggcc ggaagacccg tttcgcgcgc
     1561 gagcaaatcc agatcagaag aagaaagggg agagccaaaa agctcaagca tggccagatc
     1621 ctgctcggga tgagagtaag aaacggacgg atcaatgagg actggattca tgtctgaatc
     1681 tatgagcagg tttccgttcc ataaatcccc atgaatcagt cgcggacctg gctggctcaa
     1741 ttcccatcca gacgcaatgt ccaggactcg tcgcgcattt tccagatcct tttgggtcaa
     1801 aagcccggca gagatcacca tgttgagctg gggaaaaata cgatcctgag cccagaactc
     1861 ctcgaaagtc gcataacgtc tatttctctg atgcaggctg cctatgtagt tgtcgcgcga
     1921 ataaccataa gattcacctc gggtagagta gatctggatc agtgactcaa ggcgaacgga
     1981 acctctgcca ccggaaacca tcgacaggaa aagaagagcg ccggatcgtc cacctgaatc
     2041 cgtttttaca aaaccaatca attcaggaac caccgcaccc gcctctcgaa gtcgaaccag
     2101 accgtcccac tccgcttccg ccatatccag ggaagataca cactttacaa tgagagatcg
     2161 cgcaggcgcg cgcgaagtgg aaggaaggtc tacgcgaaat agttcaaata acccggatgc
     2221 aaaaggaaag acggcaacgc cggaagcacg cgcggcatca gagagaccaa gatccacgat
     2281 gccgcgtcgg atctgatctt tcatcaatcc atcgagcttt gcttgccttc aattacttgc
     2341 agaagaacga cagccgcaat cccgagcctg cgatcgagcc tgccagaatc catagaaaaa
     2401 tcgacgttga actgaggtac gaatggattc caggtttgct tgagatctcc cacctgagtt
     2461 ccgcccactg tcattgcaaa atgttgcgga attagattgg agagaaaacg gcgcagaaga
     2521 gccataagca tgctatcttc tgaaacggtt ccgaattcct tgtccgatgc atccagtatg
     2581 gcccactcat ctcgcagtat agatttaaga agttttctgc gaatggcacc taccttctgg
     2641 ttcgtgacca cgtcggtcac gtcataggtc gtgccaaagt ccagaatgct ccttgcctgg
     2701 attttgagaa gaggacgcga ttgagattcg tctgcgaaaa cggtaatgtc ttccttcagc
     2761 ttgaaagctt tttgtttcac aaagaaaagc agattggttc tgctttcatc atagatacgc
     2821 acttctccgc caaacagctt gagcagtttc tttttcgcat aatacttgtt agctgtaaaa
     2881 ggatccatga attacgctcc ctctttaggc gccgcttctt caatttcctg acgaaggtgc
     2941 ggatctgaaa tttgacacaa tagcttttcg cgaattacac gcggatgatc cccactctga
     3001 atagacatta cacctttgat gatgatattg cgatgagtca cggatctgtg acttaagaat
     3061 ttcaacttgt tggccatggg gagccagaga aggttcgcaa aaccaatccc gtaaaacgta
     3121 gcaatgaacg caacagcaat gcctcgaccg agctgttcaa taccaccacc accgccaagg
     3181 ccttcaagta cgtgcacgag acccataacc gttccaatga tcccaagagt tggggagaaa
     3241 ccaccgagag tttccatcaa ttcaggagga atcttatcgt gttctttttc cgcatctgcg
     3301 tagtcttcaa gaatatctcg cacaatctcc gggtcagtac catccacgac gagttccatt
     3361 ccagttcgca tcatcttgtc tttgaaactg gaaagatcat cttcgagact gagcagacct
     3421 tcgcgacgag ctctttcaga aagattcaca aacaactcga ccatttcttt caagttttcc
     3481 tttggaggaa aaattgcttt catgaagtac tgaggtatgg ctgttacttc tttcaaactg
     3541 aaggaaacca ccgcaaccgc gacagaacca ccgagaatca gaatcaaggc tccgaagttg
     3601 atgaatgacg ctacgtgacc accctcgatg aggagagtca agagaacgac cgtgaaacct
     3661 cctacgaggc ctaagactgt tgatatttcc atgctcggga ctccgctact ttactttgag
     3721 cttttctcca gttacaatga tgatgtcaat gcggcgattg agggcgtgcg attcgggagt
     3781 tccctcgcgg tccagaggac gagtacttcc aaaggaaacg gcactcatct ttcgcggatc
     3841 cacatcacgc gtttcaagaa ataaaatcac attcacagat cgcttagcac ctaactccca
     3901 gtcagacgct gagattcccg ctccgttgtt gtcctggact tcgtcgtctg tatggccttc
     3961 aatgcgaacc ggatattcca cctcagccag aagaagtgac atcttttcaa gaagctgctg
     4021 cgtttcctct gtgagctcgg aagatcctgg acgaaagtta tccattccga tcagacttac
     4081 aaccactccg cgttcgtctt cagtaacctt gatcaatttc tggagcatct cctggcgaaa
     4141 gagccccgtt attcgcgcac ggacacgccg cgctcttaca ccttccggaa aaagatcctc
     4201 gatgatggtt ctgccgccac tcagacttcc ttcgtcaatg gtggccccac cctggaaccc
     4261 catgcgaaga gattcggcca ctgccttcat tttgtttgta tccacctggg acattgcgta
     4321 gagaattacg aatacaccga acagcagggt caccatgtcc aggtatgtga gcatccatcg
     4381 cccactgccc gactcgccgc ttgctttatg ctgttccggt gccttgttct tgcgggccat
     4441 gaattatgtc gcatcatagg ggtctgtaca atcctgtcaa attcaatcgg gcccctggcc
     4501 ccgtcaggac cgcaaaaaac agcctgtttg acgcactttt gcgctttttc ctggtggaac
     4561 agtaaagatt ggcgcaaatt gagcgataca gagtgttggt aggatataac atgcgccgaa
     4621 tcgtctggac cctcgttcta tttacagcca ctgtatcagc gaactcttct gaaaagagag
     4681 cccttctgct tccagtgcag gccatcttca ttcatgaatc catgcctgca cgcccggagg
     4741 cagcgctcgg tcttcagatt gagcgcggtg catccatgat gattcagtca cgcggactgc
     4801 gcctcacggg agcatccagc gccaccatta caaaacctga accaggcctg gtgcaaagcc
     4861 ttgcagccaa acacggagtg gatctggttt actgggtaga agccactcaa aaagaactga
     4921 actaccccag agaaaactcc gcgcaaactg ctttctgcga cgatagcgaa aaagacgaac
     4981 caattcgcgg atctctgagc attaaggtgc gaatcttcga tgcagaaggt aaagaggtct
     5041 ggactgcaag cagcacgaac gactatcgct gttcatttcc ggttacggaa gcgtgggacc
     5101 agaccatgcg tttgcttga
//
LOCUS       DAOQJL010000257         5118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9544_length_5118_cov_4.702745, whole genome shotgun sequence.
ACCESSION   DAOQJL010000257 DAOQJL010000000
VERSION     DAOQJL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9544_length_5118_cov_4.702745"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLG78_11565"
     CDS             <1..951
                     /locus_tag="PLG78_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE23799.1"
                     /translation="KEDLLVVPDRNKAAAAGTSISVIGGVIRTLLSGSIVTKYYDGDR
                     EVDVRLRSEPGMFVSRDDLGTLMIPGQHGSSPLGSLVTTEQGEGDANIYRKNKRTTAS
                     ITIRVSGRSLEDVAEDADRILSASKMPGDTVYAFGKEYRKRMQSQKQMLLAVAVSILA
                     VYLLLGSLFESFIRPFLILLTVPLSIAAVLAAFMIGRSPLNMSVYIGMIMLAGIVVSN
                     SILVASTIHKRVSLAGSLVRSVLSASRERLRPVLITTTASVSGMLPMALDGSSGAALW
                     RPLALTVATGLTFSLAVSLLIVPLACLLYYNRRSQRPLGA"
     gene            954..1733
                     /locus_tag="PLG78_11570"
     CDS             954..1733
                     /locus_tag="PLG78_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23800.1"
                     /translation="MDQSQTEEILSPEDPTAIYREVTFTGPAVTFYDRLARAAHANKR
                     KIYFLMLCAIVVPIFLGLRSTRPLTASRIATDYFDRFRTGESNGVYSVAGATFGQDHL
                     RHLFDAVGSTTLGPAGLRQMDGNSAAYSAYVRDQFETDLLVHAALQEGVLDSPQASFF
                     IENSMRHSMADYYLLTRIMRPDTNLRVVVSDQAVQSYYEKNREQYAKSGLPQKETMEI
                     IRRTLEQIQSDQMQAQIRLLRQQTINSLKDRGEHHFTGVSQ"
     gene            1730..2404
                     /locus_tag="PLG78_11575"
     CDS             1730..2404
                     /locus_tag="PLG78_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23801.1"
                     /translation="MIQSMSTFWFALRKPQATWASVSGAFLEILKGFLLIAGASSLLY
                     FVTTIPNLEKLRLAAMSLQARTGFASGGIMGSIGASLPWNRLLLLPVWTAGVFTASFV
                     RFMTLRFLGEKSATSGDIQLSKIASISMLGIAPLLVANALLACLGNFLPVSDIRQANP
                     VAFASVLAFVAGVFLEAHITIRIFRAEFSQTRGRAILTWMSPYLAGFCTCSVPTITML
                     FLMAVR"
     gene            complement(2448..4169)
                     /locus_tag="PLG78_11580"
     CDS             complement(2448..4169)
                     /locus_tag="PLG78_11580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNE23802.1"
                     /translation="MRTRTIVAIAWTFLYSGPAWSQTVQGRVFDESGRPVAGAIVTVF
                     KEGSTIQESGYSDASGHFLVSSSFDGLRVRVRRPGYVDSIVPSASASSITLKAADAQA
                     LSDSLTASAHAAHVSWDSPEHKANFVSQCHYCHQIGNALTRAPKDQDTWKLTIDRMQG
                     YGALISYSDESSFRETLARSFDGKPIPFHQKHVVSPETFGAKITEWQMGEPDSFMHDA
                     EIHPNGKIYAVDMSKDKIYELDRKTNRIVAYDLPPSELPLGGMFSGAFRPLGTFAAHH
                     GPHSIQVGPDGKLWTTNSLAAEIMSFDPVTHAFKVYPVPDAIYPHTLRFDQKGRIWFT
                     VALSNKVGRFDPASGEFKILKLDSHGLFRLMSDLLLPTILKVSGWFPQKNLHVSLSHH
                     KATGEGHRILNLPYGIDVNPVDGSIWFSKLYGGFIGRVDPETLEVREWSSPLGGPRRL
                     RFAKDGTLWVPSFETSSLLRISTSTLKYKIYKLPLLNETEYETPYALGVHPVTQDVWI
                     TSNLSDRIFRFVPATEKFITYESPTRVTYMRDVLFDSDGTICTTNSNMPAYAIEGGRQ
                     KVFCLKP"
     gene            complement(4166..5047)
                     /locus_tag="PLG78_11585"
     CDS             complement(4166..5047)
                     /locus_tag="PLG78_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolaconase/LRE-like region"
                     /protein_id="HNE23803.1"
                     /translation="MSRLSRVGVYEIHISTKEVLPVLTDVLIPPDVQIDPAKPGIVYK
                     EGSAPKLKVSEFNASNSRPLAFCNDLAISPDAKRIYITTPYSYEGAAMGGGAFGEAIT
                     LARNGLLWRYNLENKTVELIARDFNFIDGILTERSDHSGEDSVLITETTKFRIDRLHL
                     RGPKAGTHDVLWEYLPGMPDGLDRDSEGRIWVGIIKKRTGFITWAHRARWIKFSLLNF
                     PQNWLPIPRQTAVLALSADASTPLFFTEHDGSGLGEISAVIPGKQHLYLANFRKNSRG
                     VYSIPYPKGLQPEGEQK"
BASE COUNT         1201 a         1324 c         1288 g         1305 t
ORIGIN      
        1 aaggaggatc tgcttgttgt gcccgataga aacaaagccg cggccgccgg aaccagcata
       61 agtgtaattg gaggtgtaat tcgaactttg ctctctggtt ccatcgtaac caaatactac
      121 gatggagatc gagaagtgga cgtgcggctg cgcagcgagc ctggaatgtt tgtaagcaga
      181 gacgatctgg gcaccctgat gattccagga cagcatggat cttctcctct ggggagtctt
      241 gtgaccaccg aacagggaga aggtgacgcg aacatttata gaaagaataa gagaaccacc
      301 gcatccatca ccattcgagt atcgggacgc tccctggaag atgtcgcgga ggacgcagac
      361 aggattctat ctgcgtcgaa gatgcccggg gacactgtgt acgcgtttgg aaaggaatac
      421 cgcaaacgaa tgcagagcca gaaacaaatg cttctcgctg tggcggtttc cattctcgcg
      481 gtctacttac ttctgggttc tctttttgaa tcattcattc gcccttttct cattctactc
      541 actgttcccc tttcgatcgc cgctgtactg gcagccttca tgattggcag atcgccgctg
      601 aacatgagcg tatacattgg catgatcatg cttgcaggca ttgtagtatc gaactctatc
      661 ctcgttgcgt ccacaattca taagagagtt tcactggcgg gcagcctggt gcgatccgtc
      721 ctgagcgctt cgcgtgaaag actcaggcca gttctcatca caacgactgc atccgtgagt
      781 ggaatgcttc ccatggcgct cgacggaagt tctggagcgg cactctggag accgctcgct
      841 cttacagttg caactggcct tacgttttcg cttgcggtga gtttactgat cgtcccactc
      901 gcctgccttc tctattacaa tcgtcgcagt caaagacctc tcggagcata acaatggacc
      961 aaagtcaaac agaagaaatc ctatccccgg aagacccaac tgccatctac cgcgaagtaa
     1021 catttaccgg accggccgtg acgttctacg atcgtctggc tcgcgcggca catgccaata
     1081 agagaaaaat ctacttcctg atgctctgcg cgattgtagt tccaatcttc ctgggcctga
     1141 gatctacgcg acctctgacc gcttcgcgca tagcaactga ctactttgat cgatttcgca
     1201 caggagaatc taacggcgtg tactcagtgg ccggggcaac ctttggccag gatcatttgc
     1261 gtcatctttt tgatgctgtg ggatccacca ctctgggtcc ggccgggctc agacaaatgg
     1321 acggaaactc tgcggcctat agcgcgtatg tgagagatca attcgagacg gacctcttgg
     1381 ttcatgcagc tttacaggag ggggtactcg attcgcctca ggcttctttc ttcattgaaa
     1441 attcaatgag acactctatg gcggattact atcttctgac gcgcatcatg cgtcccgata
     1501 caaaccttcg tgttgtggta agtgatcagg cagtgcaatc ctactatgag aagaatcgcg
     1561 agcagtacgc gaaatcagga ctccctcaaa aggagaccat ggaaatcatt cgccgaactc
     1621 tagagcagat tcaatcggac cagatgcaag cacagattcg cctgctgcga cagcaaacca
     1681 tcaactcact caaggaccgc ggcgaacatc acttcactgg agtttcacaa tgattcaatc
     1741 tatgagcaca ttctggtttg cccttcgcaa gcctcaagca acctgggcga gcgtctcggg
     1801 tgcattcctt gaaattctga aaggattcct gctcatcgca ggagcctctt cccttctcta
     1861 ttttgtaacc accattccca acctggaaaa actgcggttg gccgccatgt ctctccaggc
     1921 acgcactgga tttgcaagcg gtggaattat gggaagcatt ggtgcaagcc tgccctggaa
     1981 tagactgctt ttacttcctg tgtggactgc aggcgtgttt acagcctcct tcgtaagatt
     2041 tatgaccctt cgcttcctcg gagaaaagag tgcaacgtca ggcgacattc agctatcaaa
     2101 aatcgcatct atctccatgc tgggaattgc acctctgctg gttgcaaacg cacttctcgc
     2161 atgcctcggc aatttcctgc cggtttcaga cattcgccag gcaaatcctg ttgcatttgc
     2221 gtccgttctg gcatttgtcg caggagtttt tcttgaagct cacatcacaa tccgcatctt
     2281 tcgcgctgag ttttcacaga ctcgcgggcg cgcgattctc acctggatga gtccttacct
     2341 ggcagggttc tgtacctgtt cggttccaac cattacgatg ctatttctga tggcggttcg
     2401 ctgaaccgcc gcctcctacg cccgcccttt gcgcgctcgc attttcctca aggcttcagg
     2461 caaaacactt tctgccttcc tccttcaatc gcataggcag gcatatttga attcgtcgtg
     2521 catatggttc cgtccgaatc aaacaacaca tcgcgcatgt atgtgactct cgttgggctt
     2581 tcgtaagtga taaacttttc tgtcgcaggc acaaatcgga atatgcgatc cgacaggttc
     2641 gatgtaatcc atacgtcctg tgtgactgga tgcacgccca gagcgtatgg agtttcgtat
     2701 tcagtttcgt ttaacagcgg taatttgtag attttgtatt tgagagtgct ggtgctgatg
     2761 cgaagaagag aactggtttc aaaggaagga acccagagcg taccgtcttt tgcaaatctc
     2821 aggcgacgcg gaccacctag aggactggac cattcccgaa cttccagagt ctctggatcc
     2881 actcttccta tgaaaccacc gtacagtttg ctgaaccaga tcgaaccatc cactggattg
     2941 acgtcgatcc catacggaag attgagaatt ctatgcccct ctcccgttgc cttgtggtga
     3001 gaaagagaaa cgtgaagatt cttctgtgga aaccagcccg aaactttcag gattgtggga
     3061 agaagtagat cgctcatcag gcgaaacaat ccgtgcgaat cgagtttgag gattttgaac
     3121 tcgccggaag ctggatcgaa tcgcccgact ttgttcgaaa gtgcgactgt aaaccagatc
     3181 ctgcccttct gatcaaaacg cagagtgtgc ggatagatcg catcgggcac aggatacact
     3241 ttgaatgcgt gagtgactgg atcaaatgac atgatttccg cagccagaga attcgtggtc
     3301 cagagttttc catccgggcc aacctgaatg ctgtgaggtc cgtgatgcgc ggcgaacgtt
     3361 ccaagcggcc tgaatgcgcc ggaaaacatt ccccccagag gaagctcact cggcggcagg
     3421 tcatacgcga caatccgatt cgttttgcga tcgagctcat agatcttatc tttgctcatg
     3481 tccactgcat agattttccc attgggatga atctctgcat cgtgcatgaa ggaatccggt
     3541 tcacccatct gccactctgt gattttcgcg ccgaatgttt caggagacac aacatgcttc
     3601 tggtgaaacg gaattggttt tccatcaaag gatcgggcca gggtttctct gaaagaggat
     3661 tcatctgaat aggaaatgag cgcaccgtat ccctgcatgc gatcgatcgt aagtttccag
     3721 gtatcctggt ctttgggtgc acgggtgagt gcgtttccaa tctgatgaca gtagtggcac
     3781 tggcttacaa agttggcttt gtgttctgga gaatcccagc tcacgtgagc tgcgtgagca
     3841 ctcgcggtta aactgtcaga caatgcttga gcatccgccg ctttcagagt gatcgaagag
     3901 gcagaagcag atggaacgat cgaatccacg tagcccggtc tgcgcactcg caccctgagg
     3961 ccgtcgaagc tggaagacac caggaaatga ccgctcgcat ccgaataacc tgattcttga
     4021 atggtcgatc cttctttaaa aacggtgacg atggcgccgg ccactggtct accggactca
     4081 tcgaacactc ttccttggac tgtttgagac catgccgggc ccgaatacaa gaaggtccag
     4141 gcaatggcga ctatcgttcg tgttctcatt tctgttctcc ttctggttgc aaaccctttg
     4201 gataaggaat gctgtagacc cctcgagaat tctttctgaa atttgccagg tacaaatgct
     4261 gtttgcccgg gatgactgct gaaatctcac cgagtccgct tccatcgtgt tcggtgaaga
     4321 acaaaggtgt ggacgcgtct gcagagagtg ccagcaccgc ggtttgtctg ggaataggaa
     4381 gccagttttg cgggaaattc agaagtgaga acttgatcca gcgcgccctg tgagcccatg
     4441 tgatgaatcc cgttcttttc ttgatgatac caacccagat gcggccttct gaatcgcggt
     4501 ccagtccgtc cggcattccc ggaagatatt cccacagaac atcatgtgtg cctgctttgg
     4561 gcccgcgcaa atgcaatctg tctattctga atttcgtagt ctcagtgatg agcacggagt
     4621 cttcgccgga atggtcagat ctttctgtga gaatgccatc aatgaagttg aaatctcgcg
     4681 caatgagttc caccgttttg ttctctagat tgtatctcca tagtagaccg tttcgcgcca
     4741 gagtgattgc ctctccaaag gctcctccgc ccatcgcggc accttcatac gaatacggcg
     4801 ttgtgatgta gattcgtttg gcgtccggag aaatggcaag atcgttgcaa aaagcaagag
     4861 gtcttgagtt cgaggcgttg aattcagaca ctttgagctt tggagccgac ccttctttgt
     4921 atacgattcc tggctttgct ggatcgattt gaacgtcagg cggaatcaga acatcggtga
     4981 gtacagggag aacctctttt gtagaaatat ggatctcata tactccaact cgacttagcc
     5041 tggacaactg aattcaaacc ggagggctgg ttgaagatcc cgggagaaga agacgggact
     5101 cggcccgtgc ggctttca
//
LOCUS       DAOQJL010000258         5112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9559_length_5112_cov_4.147321, whole genome shotgun sequence.
ACCESSION   DAOQJL010000258 DAOQJL010000000
VERSION     DAOQJL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9559_length_5112_cov_4.147321"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /locus_tag="PLG78_11590"
     CDS             <1..1999
                     /locus_tag="PLG78_11590"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE23804.1"
                     /translation="EALATRLVGTTETIVPGYEDAYYYLWKEGNLPADVDPMRKDLSS
                     ESERNRLRILLERGMGSITGFALPLNFDGSLQSWTTGSWHFRRERMYLIPGDSAMGYR
                     LPLESLSGTDIGFPELSPMQEKSPFPDVAARTLRRAQTILATSGDSSSQTQIPMIHMR
                     TSVCVETRDGRLYVFLSPQWDAANYVDLIASVEDTAAELQMPVLIEGYEPPTDSRLGR
                     IAVTPDPGVVEVNIHPSDSFEEHARKLEILHEEARKCGLTTEKFLHDGRRSGTGGGNH
                     ITLGGPTPMESPFLQRPDLLRSLITYFQHHPALSYFFSGQFIGATSQSPRIDETNPES
                     LYELAIAFSQLPRSAVPVPGDAAPWLVDRLLRNLLVDQMGNTHRAEISIDKLHSPDSL
                     TGRLGIVEFRGFEMQYHAQMNSLQHLLMRALVAKFCKEPYRSAPMEWGFMLRDQYRLP
                     HFLWEDLRSIVSDLARAGYDFEESWFLPFLESRFPVYGTSQVEDCTIELRQALEAWQV
                     LGEEMSAVGTARAVDSAVERLQVRVDHFHPERYALLCNGRPVPLQATSHTGTYVAGVR
                     FKAWNPPRTLHPSIPVNSPLFFNLYDRVRKSFVGGCTYHVFHPGGRSYDTSPVNEREA
                     ESRMLSRFFPGSPAPSEFALYEENPEYPNTLDLRAGRLRDR"
     gene            1996..4479
                     /locus_tag="PLG78_11595"
     CDS             1996..4479
                     /locus_tag="PLG78_11595"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE23805.1"
                     /translation="MSASLQTKMSILESYRTSGGRDELIDENGAFRGHWNFLVDALES
                     MGPRALAGRQEEIHRFIKENGVTYHVYDEGGGKERPWPLDIMPVLIDSREWATVESGL
                     IQRAELFDLILQDLYGPAKLLKEGVLPPEIVFDHGGFLRSCHGLIPPNRRYLHFYAAD
                     LARSSNGSFTVISDRLQSPSGAGYALENRIALSRTLPSLYRDSHVHRLALFFRTLRNH
                     LSQLAGGGSRLVILTPGPENETYFEQAFLANYLGFNLVQGSDLTVRDGKAYLKTLNGL
                     RPIDGIFRRIDDAYLDPLELKNDSLLGVAGLMYCIRSGSVAVMNPPGAAVLENPGIYP
                     FLPRICKFLTGQELRLAGAPTYWCGDSLSSVLSRLSDLVIKPIARGWERGTIYCSRLS
                     SAELEDVRRRIQANPAGFIAQEKLPLSTTPLWTGEKFEPRPMVIRSFACARDESFMIM
                     PGGLVRVSPDPDSTVVSNQKGGVSKDLWILASEPEKQISLLSTSMSTPIVRSGGEIPS
                     RIADNLFWIGRYAERTEALARILREVYLRLVEMQGDEEVFDILLLALEPHCGLLDPEL
                     VRKHSGSPEVERKLRAIVFDASILGGLKFDVRALNTCGRALRERLSEDAWRTLNRLEG
                     ELDRPDKSILETLESTLLYLASFAGFVSESMTRGQGFRFLELGRRIERAGLAADLLMH
                     ATRSKAPDALWEHVLRIQNSYMTYRRRYRTRMDPIAVVDLLVFDESNPRSVAHQLIRL
                     EDLTRGLPEWKEAGKSAESRIALHALSLMRLADGAVRSADDDYRELQSLLSQEKNYLR
                     TLAETISLHYFSYIETQQTLEANQWSVTG"
     gene            4461..>5112
                     /locus_tag="PLG78_11600"
     CDS             4461..>5112
                     /locus_tag="PLG78_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE23806.1"
                     /translation="MVRYRIVHKTTYQYSEHVSLCHNEAQLSPRMTDFQIPRDTRLFV
                     SPQPAVLSERTDYFGNRLFYFAVQSPHNVLEVTSESVVDLLPRTGPAGSNPILPPSPA
                     WETVRDDVRSSWDLFQYLNESTHVRATDAVRQFALVSFFPGRPILEAAWDLNVRIHSE
                     FRYEPGSTRITTTTDEVLRIRKGVCQDFSHLAIACFRSLGLPARYVSGYIESAPQRG"
BASE COUNT         1159 a         1280 c         1360 g         1313 t
ORIGIN      
        1 tgaggctctc gccacaagac tcgtaggcac caccgaaacg attgttccgg gctatgaaga
       61 tgcgtactac tatttgtgga aagaagggaa tctgccggcg gatgtagatc caatgcgaaa
      121 ggatttgagt tctgaatccg aaagaaatcg ccttcgcatt ttgctcgaaa gaggaatggg
      181 cagcatcact ggctttgctt tgccgctgaa ttttgacggt tcactgcaat catggactac
      241 cggcagctgg cattttagaa gagaaaggat gtatctgatt ccgggcgatt ctgcgatggg
      301 ctatagactt cctctggaaa gtctatccgg caccgacatt ggctttccgg agctttctcc
      361 catgcaagag aagtcgccgt ttcctgatgt ggcagcgaga acgctacgtc gagcacagac
      421 cattctcgct acatcgggcg attcatcttc gcagactcag attccaatga tacatatgag
      481 aacatccgtc tgtgttgaaa cacgagacgg aaggctgtac gtatttctgt ctccccaatg
      541 ggacgcggca aactacgtag atttgatcgc aagcgttgag gacactgctg ctgaattgca
      601 aatgcccgtt ttgatagagg gctacgagcc accaactgac tctcgtcttg gtcgaattgc
      661 ggtgactcca gatcccggcg tagtggaagt caacatacat ccatctgatt cctttgagga
      721 acacgcgcga aagttggaga tccttcatga ggaggcgcgc aagtgtggcc tgactacgga
      781 aaagttttta cacgacggaa gaagatcagg cacgggcgga ggaaaccaca tcaccctggg
      841 tgggccgact ccgatggaga gtccatttct acagaggccc gatctgctgc gaagcctgat
      901 cacgtacttt caacaccatc ccgcactttc ctactttttc tccggccagt tcattggcgc
      961 gacgagtcaa tcgccgcgaa tcgatgaaac caatccggag agtctgtacg aactcgccat
     1021 cgcattcagt cagcttcctc gttccgcagt ccctgttccc ggagatgccg caccgtggct
     1081 tgtggataga ctccttcgca atctgctcgt ggatcaaatg ggaaatactc atcgagcaga
     1141 gatcagcatt gataagttgc attcacccga ttcgctgact ggtcggcttg ggatagtgga
     1201 gtttcgcggt tttgaaatgc aatatcatgc gcagatgaac tccctgcagc atcttctgat
     1261 gcgcgctctg gttgcgaagt tttgtaaaga gccatatcgt agcgctccga tggagtgggg
     1321 ctttatgttg cgagatcagt acaggctgcc tcactttctc tgggaagatc tgcgatccat
     1381 tgtgtctgac ctcgctcggg ccggttatga tttcgaagaa agctggttcc ttccatttct
     1441 agagagtcgg tttcctgtgt acggtacgtc tcaagtggaa gattgcacca tagaattgag
     1501 gcaggcattg gaggcctggc aagttctggg agaggagatg agtgctgtgg gaacggcaag
     1561 agccgtggat tcggctgtgg aaaggctcca ggttcgagtg gatcactttc atccggaacg
     1621 ttatgcgctc ttgtgcaacg gcagacctgt gccccttcaa gcaacatctc acaccggcac
     1681 ttacgtggcc ggagtccgtt tcaaagcctg gaatccgccg cggactctac atccgtctat
     1741 tcctgtgaat tcgcctttgt tctttaatct gtatgatcgc gtacgcaaat cattcgttgg
     1801 cggctgcacc tatcatgttt ttcatcccgg aggccgcagc tacgatactt cgcctgtcaa
     1861 tgagagagag gcagaatcca ggatgctttc caggttcttt cccggaagtc ccgcgccatc
     1921 cgagtttgcg ctttacgaag agaatccgga atatccaaat acattagact tgcgcgccgg
     1981 aaggctacgg gatagatgag cgccagtttg cagacgaaga tgagtattct ggaatcgtat
     2041 cgcacgtccg gcggccgcga cgaactcata gatgagaacg gagcgtttcg cggtcactgg
     2101 aatttcctgg tggacgcgct ggagtccatg ggcccgcgcg ctcttgccgg aagacaggaa
     2161 gagattcacc ggttcatcaa agagaacggt gtcacctacc acgtgtacga cgaaggcggt
     2221 ggcaaagagc ggccatggcc tctggatatt atgcctgtgc tcattgacag tagagaatgg
     2281 gccactgtag aaagtggtct gattcagagg gcagaactct ttgatttgat tttgcaggat
     2341 ttgtatgggc ccgcgaaact tctaaaagag ggagtgctgc ctccggaaat cgtgttcgat
     2401 catggtggat ttttgcgctc ctgtcacggc ctcattccgc ctaaccgccg ttacttgcac
     2461 ttttatgccg cggacctggc gcgatcctcg aatggttcct tcactgtgat ttcggacaga
     2521 ttgcagtccc cttccggtgc aggttacgca cttgaaaacc gaatcgcact ttctcgcact
     2581 ctacccagtc tctacagaga ttcgcatgtt cacaggcttg cgttgttctt tcgcactctc
     2641 agaaatcatt tgagccagct tgcaggcggc ggaagtcgtc tggtgattct tactcccggt
     2701 ccggaaaacg aaacctattt tgagcaggca ttccttgcca attacctggg attcaatcta
     2761 gtgcaaggct cggatctgac ggtccgtgat ggaaaggcgt atctcaaaac attgaatgga
     2821 ctcaggccca ttgacggaat ctttcgcaga attgacgatg cttacctgga tcctcttgag
     2881 ttgaaaaacg attctcttct tggcgtggcc ggtcttatgt attgcataag atctggatca
     2941 gtggccgtaa tgaatcctcc gggtgctgca gttcttgaga atcctggaat ctatccattc
     3001 cttccgcgaa tctgcaaatt tctgacgggg caggaactgc gtctggccgg agcgcccacc
     3061 tactggtgcg gcgattctct ttcttctgtt ctctctcgtt tgtccgattt agtaatcaag
     3121 ccaattgcgc ggggctggga aagagggacg atctattgtt ccagattgtc ttccgcagaa
     3181 ctggaagatg tcaggcgtcg gattcaggca aatccagcag gcttcattgc tcaggaaaaa
     3241 ctccctctct ctactacacc actctggaca ggcgaaaaat ttgaacctcg tcctatggtg
     3301 attcgcagct tcgcctgtgc gcgtgatgaa tcatttatga tcatgccggg cgggctggtt
     3361 cgagtatctc cggatccgga ttctactgtt gtttccaatc aaaaaggtgg agtgagcaag
     3421 gatctctgga ttcttgcatc tgagccagag aagcaaatca gcctcctgtc tacttcgatg
     3481 tccacgccga ttgtacgttc cggtggagaa attccttcgc gcatcgcaga caatctgttc
     3541 tggattggac gttatgcgga aaggactgag gcgttggcgc gcatactgag ggaagtgtac
     3601 cttcgtcttg tggaaatgca gggagatgag gaagtattcg atattctgct tctggccctg
     3661 gaaccacatt gcggtcttct ggatcctgag ctcgtgcgga agcattccgg ttcgccggaa
     3721 gtagaacgca agctcagggc gatcgtattc gatgcgtcca tccttggtgg gctcaagttt
     3781 gatgttcgcg cgctcaacac atgcggaagg gctctgcgcg aaagactgtc agaggatgca
     3841 tggagaactt tgaatcgcct cgaaggtgaa ctggatcgtc ctgataaaag cattctggaa
     3901 actctcgaaa gcactcttct gtacctggca agctttgcag gtttcgtgag tgaaagtatg
     3961 acgcgcggcc aggggtttcg gttcctggaa ctgggtagaa gaattgagag agccggcctt
     4021 gccgcagatc tgctgatgca tgcgaccaga tccaaagcgc ccgacgcact ctgggaacat
     4081 gtccttcgca ttcagaattc gtacatgacc tacaggcgca gatatcgcac acgaatggat
     4141 cctatcgctg ttgttgatct acttgttttc gatgaatcca atccacgctc cgtcgctcat
     4201 caattgattc gcctggaaga tctaactcgc gggcttccgg aatggaaaga ggcgggcaag
     4261 agtgcagaaa gtagaatcgc cttgcacgcg ctttccttga tgcgcctcgc ggatggagcg
     4321 gttcgatccg ccgatgacga ttatcgtgaa ctacagtctt tgctttcgca ggagaagaat
     4381 tatcttcgga cgctcgcaga aacaatcagt ctgcactatt tcagttacat cgaaacacag
     4441 cagactctgg aggccaacca gtggtccgtt acaggatagt tcataaaacc acatatcagt
     4501 attctgagca tgtttcttta tgccacaatg aagcgcagct cagtccgcgc atgacggatt
     4561 ttcaaatccc cagagataca cgtctttttg tttctccgca gcctgcagtt ttgagcgaaa
     4621 ggacggacta tttcggcaac aggctattct attttgcagt tcaatcgccg cataacgttc
     4681 tggaagtaac ttcagaaagt gtggtggatc ttttgcctcg caccggaccg gcaggttcca
     4741 atccgatttt gccaccttcc cccgcctggg aaactgttcg cgacgatgtt cgatcttcct
     4801 gggatctctt tcaatacttg aacgagtcta ctcacgttcg ggcaacggac gcagttcgcc
     4861 agtttgctct ggtctcattt tttccaggca gacccattct ggaggctgcg tgggatttga
     4921 acgtgcgaat tcattccgaa ttcagatatg aacccggttc cactcgcatc acgacgacta
     4981 cggatgaagt gttgaggatc aggaagggtg tctgtcagga tttttcgcat ctggccattg
     5041 cctgttttcg atctcttggt ttgcccgcca gatacgtcag cggctatatt gaatcagcac
     5101 cgcaaagggg cc
//
LOCUS       DAOQJL010000259         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9566_length_5110_cov_4.573492, whole genome shotgun sequence.
ACCESSION   DAOQJL010000259 DAOQJL010000000
VERSION     DAOQJL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_5110_cov_4.573492"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLG78_11605"
     CDS             <1..1293
                     /locus_tag="PLG78_11605"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNE23807.1"
                     /translation="SSVVLEDSIIKSAGRGITTGAGVRVLKGDQSGYAFSEDLTVESL
                     IRAARTAAAIADANSSIQIAPAFEGSKRNLYPASAESTLEEKIKILRDADSAARAFDP
                     SIEKVSASISDEIQTISCITSEGKVFGDVRPMVILRVSCIAQKNGKRQTGSDSAGGRF
                     GLEYFSERRTAADIAKEAARLAVLNLNAEDAPAGPMEVVLGPAESGVLLHEAVGHGLE
                     ADFNRKNLSNYSNRIGERVASEHCTIVDEGLFSNMRGSINVDDEGNEPSSAVLIEDGI
                     LRGYINDRMSAEILKTAPTGNGRRESYRCLPMPRMTNTYMRPGAHDPGEILSTVKRGI
                     YAKRFGGGQVDITKGDFVFSVLESYLIEDGKITRPLKGVTLIGNGPDVMTKVVMVGND
                     LTFTEGGWTCGKDGQSVPVGMGIPTCKVSEITVGGSRV"
     gene            1290..2666
                     /locus_tag="PLG78_11610"
     CDS             1290..2666
                     /locus_tag="PLG78_11610"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNE23808.1"
                     /translation="MTTQVQDRFQIDSLSLADDVVREVMKLGADACDVVVSEGADFSV
                     QVRLGRIEKLEESISRGLGIRVFKDGAVGLVHTTDFADKSIKELVKDAIAIARVSDRD
                     AANGLAPRELLGRYAGKLNIYDPSISAISPARKIEMARACEDAGLALDPRIKNSDGAS
                     WFDAEGRFTLATSDGFRGQYAGTTAGISASLLAEEDGVKQTDSWYSYDRFVSRLDTPE
                     AVGKEAARRTVARLGSRKVKSQTAALVVDPLVSRRFVSMIFGAASGGSIYRKSSFLLD
                     RLGTVIAAPGVTIDDDATLEDGPASRPFDGEGLPGRRLRLVENGTLGEIPCESYFARK
                     LGRSPTGNAVRGYASGPAAGSTNLFMQPGSDSPSDIIRSVKNGLYLNSLMGMGFNGVT
                     GDLSQGASGYWIENGEIAFPVQEITIAGNFLDMLAGIERIGNDLSFKMGSTAAPTLLI
                     REITIGGS"
     gene            complement(2667..3425)
                     /locus_tag="PLG78_11615"
     CDS             complement(2667..3425)
                     /locus_tag="PLG78_11615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE23809.1"
                     /translation="MLKNIRSVALIATSFFSILFCARMEAKLPSRPGMFAVIDTTQGS
                     LTIELFPDAAPKTVKNFADLAQKGFYKGIVFHRVIPDFMAQTGDPTGTGSGGPGYQFE
                     DEINPKALGLDKIKVKDAPEYGRQAQQLAVKNLDITSQSQLNAKRAQFEAEVRKIGEM
                     TVAEVLEKSGYKYQDSLPSRKAIRGAVAMANAGPNTNGSQFFINQVETPHLNGLHTVF
                     GQLEASDFEVLDKIIAAGNGNTKIKDVQVFDKRK"
     gene            complement(3455..4441)
                     /locus_tag="PLG78_11620"
     CDS             complement(3455..4441)
                     /locus_tag="PLG78_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23810.1"
                     /translation="MRTLRQWLRLITAHPAESLGIAVALGQLAFLRSLPILWLLILWI
                     PAALIAYLILRLTIPRILRRFNSIHNHWIELIVSALTLTILYLFLGLPWWSAVLLICV
                     FAFMVLAIADKRPGAFYVIYYGLLIFILFAGAHGILLAEFRTVAWLMDKHETGAMHEE
                     VKDGKWTILRGNNPILEFAILPEMKVSHDVNMEWIPGNALGVLSPGGGISPPGLIPFE
                     MTHKETGEKISFLLGGMNLSGTAESLTPPRRDSLSFSGVELQGLVFGFKNPQSGRLMQ
                     IALYCSSKFDLSFLVIEEVQPGFPHSPLILQQLDQARPGLQTGNPNCVFDNP"
     gene            4535..4858
                     /locus_tag="PLG78_11625"
     CDS             4535..4858
                     /locus_tag="PLG78_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE23811.1"
                     /translation="MHAEIHKDGTTLILRAVYPRLDGGNHLLNALKSGPWLEGDVEAV
                     EIDFGDVEYLNSLGITELVTIHRLVQEQTRGKTRFRFFNVDRKIRTILELVEVQKIAE
                     IVPRP"
BASE COUNT         1274 a         1202 c         1381 g         1253 t
ORIGIN      
        1 tcctccgtag ttctagaaga tagcattata aaatctgcag gccgcggtat cactacgggc
       61 gccggggttc gcgtgctcaa aggcgatcaa tcaggttatg cgttcagcga ggatctgaca
      121 gtagagagct tgattcgagc ggcgcgcact gcagccgcca ttgccgatgc gaatagttcg
      181 attcaaattg cgcctgcatt tgaaggaagc aaaaggaatt tgtatcctgc ctccgccgaa
      241 tccacgctgg aagaaaaaat caaaatcttg cgcgatgcag atagtgcggc ccgtgccttt
      301 gatccatcca ttgaaaaagt cagcgccagc atttcagatg aaatccaaac cattagctgc
      361 atcacctcag agggaaaggt gttcggcgat gtgcgaccca tggtcattct tcgcgtgtcc
      421 tgcattgcgc aaaaaaatgg aaagcgtcag acaggaagcg attctgcagg cggtcgtttt
      481 ggtcttgaat atttttctga gcgtagaacg gccgcggaca ttgcgaagga agcagcacgc
      541 cttgcagttc tgaatctaaa tgcggaagat gctcctgcag gtccaatgga agttgtgctt
      601 ggcccggcgg aaagcggtgt tttacttcac gaagcggtgg gtcacgggct ggaagcagac
      661 tttaatagaa agaatttgtc caattactcc aatcgaatcg gagagcgagt cgcatctgag
      721 cactgcacga tagtggatga aggacttttt tccaacatgc gcggaagtat caacgtagac
      781 gatgaaggaa acgaaccttc tagcgcggtg ctgattgagg acggaattct gcgcggatac
      841 atcaacgatc gcatgagcgc ggaaattctg aaaacagcgc caaccgggaa tggtcggcgc
      901 gaaagctacc gctgcctccc gatgcctcgc atgaccaata cgtacatgcg tcccggtgca
      961 cacgatccag gggaaatact ttctacagta aaacgcggaa tctatgcgaa gagatttggc
     1021 ggggggcagg tggacatcac caagggcgat tttgttttct ctgttctcga aagctatctc
     1081 attgaagacg gaaagatcac acgtcctctc aaaggcgtaa cgttgattgg aaacgggccg
     1141 gatgttatga caaaggtagt gatggttggg aatgatctca cgtttacaga aggtgggtgg
     1201 acttgcggaa aagacggcca atctgttccc gtgggtatgg ggattcctac atgcaaagtc
     1261 tctgaaatta ccgttggtgg ttcgcgcgta tgacgacgca agttcaagat cgttttcaaa
     1321 tagattcgct ttctctcgca gacgatgtgg tgcgagaagt catgaagctt ggcgcagatg
     1381 cctgtgatgt agtcgtttct gagggagcgg atttcagtgt ccaggtgagg cttggcagaa
     1441 ttgaaaaact cgaggagtcc atttcgcgcg gcctgggcat tcgtgttttc aaagacggcg
     1501 ccgttggact tgtccacact actgatttcg cagataaatc tatcaaagaa cttgtgaaag
     1561 acgcaattgc aatcgctcgc gtatcggatc gtgacgcggc caatggactt gcaccgcgcg
     1621 aactacttgg tcgctacgca ggcaaactca atatttacga tccgtccatc agtgcgattt
     1681 ctcctgcgcg aaaaatcgaa atggcgcgtg catgcgaaga cgcgggcctg gcactggatc
     1741 ccaggatcaa gaactcggat ggtgcatcat ggttcgatgc ggaaggcaga ttcactctgg
     1801 ctacttccga cggttttcgt ggacagtacg ctggaaccac tgctggaatt tctgcctcac
     1861 ttctggcaga agaagacggc gtgaaacaaa cggactcctg gtattcgtat gatcgctttg
     1921 tttcgagact ggatactccg gaggcggttg gaaaagaagc ggccaggaga actgtggcaa
     1981 ggctgggatc gaggaaagtg aaatcacaga ccgcagctct tgtagtggat cctctggttt
     2041 cgcgtagatt tgtaagcatg attttcggag ctgcgagcgg cggaagtatc tatcgcaaat
     2101 cttccttcct gttggatcga ctgggcacgg tgattgccgc gccaggagtc acgatagacg
     2161 acgatgcaac tctggaggat ggcccggctt cgcgtccctt tgatggggaa ggacttccgg
     2221 gacggagact cagactcgtg gagaacggaa cgttaggcga aattccgtgc gagtcctatt
     2281 tcgcgcgcaa gcttggtcgc agtccaaccg gaaacgcagt ccgcgggtat gcttccggtc
     2341 cggcggctgg aagcaccaat ctattcatgc agccaggctc agacagtcca tcggatatca
     2401 ttcgttcggt taagaacggt ctttacttga attctcttat gggaatgggt tttaacggag
     2461 taacgggaga tctatcgcaa ggagcttctg gatactggat tgaaaatgga gaaattgcgt
     2521 ttccagttca agagatcacg atagccggca attttctcga tatgcttgcc ggcatcgagc
     2581 gaattggaaa tgatctgagc tttaaaatgg gatccacggc ggctcccact ttgttgatca
     2641 gagaaattac aatcggcggt agttgactat tttcttttat caaaaacctg cacgtctttg
     2701 attttcgtat ttccgtttcc tgcggcaatg atcttgtcta gaacttcaaa atcagaagcc
     2761 tctagctgac caaacactgt gtgcagaccg ttgaggtgcg gtgtctctac ctgattgatg
     2821 aaaaactgcg atccatttgt gtttggacct gcatttgcca tggcaaccgc acctctgatt
     2881 gctttgcgcg atggcagaga atcctgatat ttgtagccgc ttttttctag aacttctgcc
     2941 actgtcattt ctccaatctt gcgaacttcc gcttcaaact gcgctctttt tgcattcagt
     3001 tgcgattgtg aagtgatatc caggtttttt acagcgagct gctgggcctg tctgccatat
     3061 tctggtgcat cttttacttt gattttgtca aggccaagag ccttcgggtt gatttcatct
     3121 tcgaattgat atccgggtcc gcccgatcct gttccggtag gatctcctgt ttgtgccata
     3181 aaatcaggaa tcacgcgatg aaacacaatc cctttgtaga atcctttttg cgcgaggtct
     3241 gcgaagttct tcacagtctt tggcgccgca tccgggaaca attcaattgt aagagaaccc
     3301 tgtgtcgtat caatgactgc aaacattcca ggccgcgatg gcagcttagc ttccatgcgc
     3361 gcacaaaata aaattgagaa gaaggatgtc gcgatgagcg cgacagagcg aatatttttc
     3421 aacataatga aaaatcgatt ctggttcgag gctgtcaagg attgtcgaac acgcaattcg
     3481 gattcccggt ttgtagaccg ggacgtgcct ggtccagctg ctggaggatc agaggagaat
     3541 gcggaaagcc aggttgcact tcttcaatca caagaaatga aagatcgaac ttgctcgagc
     3601 aatacagtgc gatttgcatg agccttccgc tctgcgggtt tttaaatcca aacacgagtc
     3661 cttgaagttc cacacctgaa aaactcagac tgtctcttcg cggcggagtc aaagattcag
     3721 cagttcctga tagattcatt cctcccagaa ggaaggatat cttttcccct gtttctttgt
     3781 gggtcatttc aaacggaatg agacctggag gcgaaatgcc acctcccggc gagagcacgc
     3841 caagtgcatt gcccggaatc cactccatgt tcacgtcgtg cgaaactttc atttccggaa
     3901 gaattgcaaa ttcaagaatc gggttgtttc cgcgcaggat cgtccatttc ccgtctttga
     3961 cttcctcatg catcgcacct gtctcatgct tatccatgag ccatgcgacc gtgcgaaact
     4021 ccgcgagcaa aatcccatgc gctcccgcga atagaatgaa gatgagcagg ccatagtaga
     4081 tgacgtagaa tgcgccgggc cttttgtctg cgatcgcaag aaccatgaag gcgaagacgc
     4141 atatcaacag caccgcgctc caccagggta ggcccaggaa aagatacaga atggtaagtg
     4201 taagggcgga aacaatgagt tctatccagt gattgtggat tgaattgaat cgccggagga
     4261 tgcgcggaat ggtgagccgg aggatgaggt aggcaatcaa tgctgccgga atccagagaa
     4321 tcaagagcca gaggatgggc aggcttctta gaaacgcgag ttgccccaga gcaacggcga
     4381 ttcccaggct ttcagccgga tgagctgtga tcagccggag ccattgtcgc aaagtgcgca
     4441 tctaatcccg aaaaacaatc tggttcctcc ttgcatctta aaatttgcat cagttttgat
     4501 ttgtggaaaa cgacaggctg cgatttctgg cattgtgcac gctgaaatcc acaaagacgg
     4561 gaccacctta atcctgcgcg ccgtgtatcc gcgtctggac gggggcaacc atctgctcaa
     4621 tgcactcaag tctggcccgt ggctggaagg ggacgttgag gcagtggaga ttgattttgg
     4681 cgatgtagaa tacctcaatt cgctcgggat tacagaactt gtgaccatcc acagactggt
     4741 gcaggagcag acgcgcggga aaacaaggtt tcgtttcttc aatgtggacc gcaagattcg
     4801 tacgattctt gagcttgtgg aagttcaaaa gattgctgaa atcgttccga gaccttaaat
     4861 tcagcctttt tcccttaata attgcagttg taggagctcc tacatcttgt gcgagctccc
     4921 agatctggca aagagaacag actttgttcg ggtttgatgt ggccgggcca gtcaatgcgc
     4981 gaacaaggga gaacctcgaa acactcgccc ggacgcggga agcctgggcc aggttttttc
     5041 caaaaacaga ggggatccgg atccagatgc acgctggaat gatgcttccc ggcgcgtacg
     5101 attcatccgc
//
LOCUS       DAOQJL010000260         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9597_length_5096_cov_3.543146, whole genome shotgun sequence.
ACCESSION   DAOQJL010000260 DAOQJL010000000
VERSION     DAOQJL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9597_length_5096_cov_3.543146"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLG78_11630"
     CDS             complement(<1..414)
                     /locus_tag="PLG78_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE23812.1"
                     /translation="MGALTIEEFLARSHEEPVLDVRTPAEFLQGHLPGAVSIPLFTND
                     ERAVIGTLYVQQGRKNAMKAGLDFIGPRMRSIVESAEQVARDGRVLVHCWRGGMRSGA
                     VSWLLSFYGFEVHTLTGGYKSFRNLALREIAKPRRF"
     gene            complement(530..1381)
                     /locus_tag="PLG78_11635"
     CDS             complement(530..1381)
                     /locus_tag="PLG78_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23813.1"
                     /translation="MRKIALLVIVGLFAAGDLSATTYKLFVHGRSGSNHCQSLTSTAS
                     GTTDYNNYWGGAVTGLSDVRYVGFDGTQSGGAYSWNSCGAQKQLNDALRVFCTGSNTC
                     EIYTHSTGGLVVAAYFGLNSPTGLNISRIQLMASAAGGSELADFSTSYLAWLGFDTLG
                     GALDQSVSTSGARNGFNHNTSGGRTYYTTSGEGTDYLYATSPLLPGYDDGVLANHSLC
                     NINGVASVGVSCTRGNGRLTESYACGFLWLSTCYNYYYRWSPYYTVWQGGSGESHGGA
                     KYDYNRR"
     gene            complement(1506..2309)
                     /locus_tag="PLG78_11640"
     CDS             complement(1506..2309)
                     /locus_tag="PLG78_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23814.1"
                     /translation="MKAPGQTKIASIMRTLPDRFWIRLHMSLILLGVTLSGIASGKIL
                     QLVGVGIVYRYAITVIVSYGVFLVLIRLWLWLVTPRITVRRTEASSQSSQSSSGSVDL
                     PDGVVLPDGILEEGEGASFSGAGGEFGGGGASASFGESASSSSGSSSSSGGGFDIGDV
                     DDARALVLLAAIAIVAALLAGVWVYIIYQAPVILTEAFLQVLLASGMFKKAKSMHSAG
                     WVGSVVGATKLPLLIVLLLSVGLAFVISTFCSEAHTIVQAVRLCIVPEL"
     gene            2411..4363
                     /locus_tag="PLG78_11645"
     CDS             2411..4363
                     /locus_tag="PLG78_11645"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE23815.1"
                     /translation="MRAGVVVLLILLGLLSCSGASGVSRATEKWQISDEVAPLSVTPR
                     SVTEPDQVIWQSIPALTRLGAKTAVIRIPVMGGAGRTLYIPASTFAMRVYCDSKIIYS
                     FSDETQFTPRHFRGWAYHVVDLDPCTQGILYFHVYSALNVRFVIPHSGTTEDVIRFLI
                     LNAIGPFIVVVICVLLGCGFFLLFLIQTDRLSGSLSLFLLCTGIWLFGLNPLSKLLFH
                     DHPLWASLAVTCLYASPAGFALFAERIMPGSILFRNGLGAAVFVLFAAAALFLDLTGV
                     IPLYRSILPFNVAVLCVALLTAVQLIRGLRHGRHEARILGIGVACLIVFAVHDMLLAF
                     STFAGVIDLRVSRLHYGMLGFILSMVGVAANQWKQMQSDIHRHSRELERRVNERTAHL
                     ESALAAIRANDVRVREEIAIASSIQRQLLLQLPIEMPGLRISGCYLPMHDVSGDFYDI
                     YYNENGSVIFFLADAVGHGVPAALIMSMLKAIFLESAPLFDDPSWILAHMNNRLEKHL
                     ARTGNYATGTVLFIPDKGPVLYSNAANRPAVILRENGTPEWLDGNGLILGAFPQQPED
                     YTLQTVELAPGDRILLFTDGITEQTFENDAFGEERLFDFLHGKSHLESDALTNAVLGE
                     LARFAHKKDFDDDVTICVMERLGKKK"
     gene            complement(4378..>5096)
                     /locus_tag="PLG78_11650"
     CDS             complement(4378..>5096)
                     /locus_tag="PLG78_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23816.1"
                     /translation="RTGRAGQEGVALTLVSPGEFRSWKRMTRENGGTCELAVIPSAAD
                     VRAKRTSGLVEKIAGTPPNHANEALLDRLTEMMDEREVGLRLLTMIQNSSEVEGPEKI
                     GKAISEVHDIAREEGRRKGGRFDRKGGGRFGRNRRDRASAHSDRQERDREGRGAGIYS
                     ASASHSAKSSHGKALNERSSHDKASHEKSAHARSSHGSSGSRKNEGKAKSGKVFRKAG
                     PGFGNHARKDRKKKGPKKKH"
BASE COUNT         1211 a         1373 c         1234 g         1278 t
ORIGIN      
        1 aaagcgtctt ggctttgcga tttctctgag ggccagattg cgaaagcttt tgtagcctcc
       61 tgtgagagta tgcacttcaa agccataaaa agaaagaagc cacgaaacag caccgctcct
      121 cattccccct ctccagcaat gaaccagcac tctaccatcg cgcgctacct gctctgccga
      181 ttctacgatc gaacgcattc tcggaccaat gaaatcaaga ccggccttca tcgcattctt
      241 tctgccctgc tgcacataga gagttcctat cacagcacgt tcatcgtttg taaagagcgg
      301 aatagatacg gcgcctggca gatgcccctg gagaaactcg gcaggagtgc gcacatctag
      361 aacaggctcc tcgtgggagc gggctagaaa ttcctctata gtgagggcac ccatggttca
      421 ttccagggtt tacgtcgggt tttgaaatcg aaatttaact gccggagtga gtgcgagggc
      481 aaaaaaaaac ccggtgattc caccgggttt catttagcag tttcaaatct cagcgacgat
      541 tgtagtcgta cttcgctccg ccatgacttt cacctgatcc accctgccat acagtgtagt
      601 aaggactcca tctgtagtag tagttgtagc aggtagacag ccagaggaat ccacatgcgt
      661 aggattctgt gaggcgccca tttccgcgcg tacaggaaac accaaccgat gcaacaccgt
      721 tgatgttaca aagactgtgg tttgccagaa cgccgtcgtc atatcctgga aggagaggac
      781 tggttgcgta gagataatct gtaccttcgc cggaagtagt gtagtacgtt cttccgcctg
      841 aggtgttgtg gttgaaaccg tttcttgcac ctgacgtact gacagactga tcgagcgcgc
      901 caccgagtgt atcaaagcca agccaggcaa gatacgaggt agagaaatcc gcaagttcag
      961 atccgccggc cgcggaagcc atcagttgaa tacggctgat gttgagtcca gtgggactgt
     1021 tcaatccaaa gtaagccgca actaccagac cgcccgttga gtgtgtgtaa atttcacagg
     1081 tattgcttcc tgtgcagaat acacgcaacg catcgttcag ttgcttctga gctccacaag
     1141 aattccagct gtacgctcca ccgctttgag ttccatcaaa tccaacataa cgaacatcag
     1201 acagacctgt tacggccccg ccccagtagt tgttgtaatc cgttgtcccg ctggcagtag
     1261 aagtgagtga ctggcaatga ttgctgcccg aacgaccgtg tacgaaaagt ttgtaggttg
     1321 ttgctgaaag gtctccagca gcgaaaagtc ctacgattac taacagtgct attttgcgca
     1381 ttgttatccc ccggtcaaaa gatgatcgag tcgcacgaaa gctggaagca tttttcatgc
     1441 attgcaccaa aaaaatttct aagcccaagt acctagactc ttcgcaggcg aagataggcg
     1501 aattctcaaa gctccgggac gatgcacaac cgcactgcct gaacgatcgt atgcgcttcg
     1561 ctacaaaatg ttgaaatcac aaatgcaaga ccaacggaaa gaaggagaac aatcaacagc
     1621 ggaagcttcg tagcgcccac cacactccct acccagccag ctgagtgcat ggattttgcc
     1681 ttcttgaaca ttccagacgc gagcagaact tgcaaaaagg cttctgttaa aatgacgggt
     1741 gcctggtaga tgatataaac ccagactccc gcaagaagtg ctgcgacgat ggcaatggcc
     1801 gcaagtaaga caagcgctct tgcatcatct acatcaccaa tatcaaatcc ccctccagaa
     1861 gatgacgaag acccggagga tgaagatgca gactcaccaa agctcgcgga agctcctcct
     1921 ccgccgaact ctccacccgc tcctgaaaag ctcgcaccct ctccttcttc tagaattcca
     1981 tccggcagta caactccatc cggtaagtcc acactaccgg aggatgattg ggatgactgc
     2041 gaggacgctt ctgtgcgcct aacggtaatg cgaggagtta cgagccagag ccacaagcga
     2101 atcaaaacaa gaaaaacgcc gtaagaaacg atcactgtga tcgcataacg gtatacgata
     2161 cctacaccca cgagttgcag tatttttcca gatgcaattc cagagagagt cactccaagc
     2221 agaatcagac tcatgtgaag cctgatccag aatcgatccg gtagtgttcg catgatggac
     2281 gcaatctttg tctgccccgg ggctttcatg ggtcaatccg gcggctgagc ggttgttcgg
     2341 gaaacaacaa atcgaacgat tcgccgggct tttcactttt ctcctcacgc aagcacgcga
     2401 agtatggcga atgcgagccg gagtagttgt cctcctgatt ctgctcgggc tactctcctg
     2461 ctcgggagct agcggcgttt cgcgtgccac tgaaaagtgg cagatttcag acgaagtggc
     2521 tccgctttca gtcacgccgc gttctgtcac agaaccagac caggtgatct ggcagagcat
     2581 tccagcgttg accagactcg gcgcgaaaac cgcagtcatt cgcatccctg tgatgggagg
     2641 agcgggaaga accctgtata ttccagcaag tacgttcgcg atgcgcgtct actgtgactc
     2701 caaaataatc tactcttttt cagacgaaac gcaattcact ccgcggcatt tcagaggttg
     2761 ggcatatcat gtagtcgatc tcgatccatg cactcagggc attctctact tccatgtgta
     2821 ttctgcgttg aacgttcgct ttgtaattcc tcacagcgga accacggaag atgtgattcg
     2881 ctttctgatt ttgaatgcaa tcggaccttt catcgttgtt gtgatttgcg ttttgcttgg
     2941 atgcggattc ttcctgttgt ttctcatcca gacagatcga ttgtccggtt ctctcagcct
     3001 gtttctattg tgcactggca tctggctgtt tggtttaaac ccactgtcca aactgctgtt
     3061 tcacgatcat ccactctggg caagccttgc tgtcacctgt ctctatgcat ctcccgcggg
     3121 ctttgctctc ttcgcagagc gaatcatgcc aggatccatt ctgtttcgaa atggactggg
     3181 ggcggccgtc ttcgttctgt ttgcggcagc ggcactgttt ttggacctca ccggagtgat
     3241 ccctctctac agaagcattc tccccttcaa cgttgcggtt ctgtgcgttg ctctactcac
     3301 tgcagtgcag ctgattcgcg ggctgaggca tggccgccat gaagccagga tccttggcat
     3361 tggagtggct tgcttgattg tatttgctgt gcacgacatg ctccttgcat tttctacatt
     3421 cgccggagtg attgatcttc gagtgagcag acttcactac gggatgcttg gattcattct
     3481 gtctatggta ggagtggccg ccaaccagtg gaagcaaatg caaagcgaca tccacagaca
     3541 ttccagagag ctagagcgaa gagtcaatga acgaactgca catctggaaa gcgcactcgc
     3601 tgccattcga gccaatgacg tgcgcgtgcg agaagaaatt gcaattgcgt cgagcatcca
     3661 gcgccagctt ctgttgcagc ttcccattga aatgccaggc cttcgcatat cgggctgtta
     3721 tctgcccatg cacgacgtga gcggagattt ctacgacatc tactacaacg aaaacggaag
     3781 cgtcatcttc tttctggcag acgctgtagg tcacggggtt cccgcagcac tcatcatgtc
     3841 catgcttaag gcaatctttc ttgaatcggc accgctcttt gatgatcctt cgtggattct
     3901 tgctcacatg aacaacaggc tggaaaagca cctcgcacgg acaggcaact atgccacggg
     3961 tacagttttg tttatcccgg ataaaggacc tgttctgtat tctaacgcgg ccaatcgacc
     4021 tgcagtgatc cttcgcgaaa acgggacacc ggaatggctc gatggcaatg gattgattct
     4081 tggcgccttt ccgcagcaac cggaagatta tacattgcag actgtggaac tggcacccgg
     4141 agaccgcatc ttacttttta cagatggaat cacagagcaa accttcgaaa acgatgcatt
     4201 tggagaagaa agactttttg atttcctgca cgggaagtct cacctggaat ccgatgctct
     4261 cacaaatgca gttctggggg aactcgcgcg gttcgctcac aagaaagact ttgatgatga
     4321 tgtgaccatc tgcgtgatgg aacgcctcgg gaaaaagaaa taggatctca cccgatctca
     4381 gtgtttcttt tttggaccct tcttctttct atcttttcgc gcgtggtttc caaaaccagg
     4441 tcccgcctta cgaaacacct tgccgctctt tgccttgcct tcgttttttc tggatccaga
     4501 agaaccgtgc gaagacctgg catgcgcaga cttttcgtgc gaagctttgt catgcgagga
     4561 cctttcattc aatgctttgc cgtgcgacga tttcgcggaa tggcttgcgc ttgctgaata
     4621 aatgccggcc cctcttccct cgcgatccct ttcctggcga tctgaatgag ctgaagctct
     4681 gtctcgcctg tttcttccaa atcttcctcc gcccttgcga tcaaatcgcc cacctttgcg
     4741 tcttccttct tcgcgcgcga tgtcgtggac ttctgaaatt gcttttccta ttttttccgg
     4801 cccttcgact tcggatgaat tttgaatcat ggtgagaagt ctcaggccta cttcacgctc
     4861 atccatcatt tcagtgagcc gatcgagaag cgcttcattt gcatgattgg gtggagtgcc
     4921 cgcgatcttc tctaccaggc cggaggttct tttcgcacgc acatctgccg cactcggaat
     4981 cacagcaagt tcacatgttc cgccattttc acgagtcatt cttttccatg agcgaaattc
     5041 gcctggagaa acgagagtca aagccactcc ttcctggccc gcgcgacctg tgcggc
//
LOCUS       DAOQJL010000261         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9660_length_5074_cov_3.947798, whole genome shotgun sequence.
ACCESSION   DAOQJL010000261 DAOQJL010000000
VERSION     DAOQJL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_5074_cov_3.947798"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1092)
                     /gene="queG"
                     /locus_tag="PLG78_11655"
     CDS             complement(154..1092)
                     /gene="queG"
                     /locus_tag="PLG78_11655"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNE23817.1"
                     /translation="MQAQAVEQTFIETALSLGFDLAAIAPISLPEKDTTAFKDFVAEG
                     RHGTMEYLARNLEMRLDPRSLMHDAKSALVLGSFYRTPESDAALKHARVKIARYAHTG
                     EYHDLLRERGRKLGRALKQKLPGLRCRFCIDTAPLAERVLGRLAGLGFQGKNTNLIHP
                     GRGSFFFISVLLIGQELKPGVPALDRCGSCRICIDACPTGALTEYKIDARKCISYLTI
                     ERRSPMPDELRPHVQGWMFGCDVCQEVCPYNRTASYGAGWPSPDPGVVRLMESASEES
                     LEDARANTPLGRPPLDLLKQSLQIAKESLDYLSEKI"
     gene            complement(1073..1885)
                     /locus_tag="PLG78_11660"
     CDS             complement(1073..1885)
                     /locus_tag="PLG78_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE23818.1"
                     /translation="MKKFWNDLPVYGSMIRAAIQSRMEYRGSFIMYILTIMGFYSAQI
                     GVLWVMVNKFNKIGTWNAGEVAFLYSLLVIATGVVSALFSGFLEFSDFVRQGTFDRVL
                     LRPLSPLFSILAMRFEPGGLANIVLGVVAFVTADRLVHIQWSPEAILMLVLVLSGGVM
                     IMGAIRIIIAATAFYTISNEGMQHLFVFSSREFLLYPVDIYNRPVRFALTFLFPLAFI
                     NFYPAHYFLNKSADSLFHPAFVYFTFPVGLVCMIIAIVFWRRAVVRYASTGS"
     gene            complement(1888..2673)
                     /locus_tag="PLG78_11665"
     CDS             complement(1888..2673)
                     /locus_tag="PLG78_11665"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE23819.1"
                     /translation="MAYFKFISMSFQRAISYRVEYFMAVLNAFLYIFIFTSVWRTLVP
                     DGSTKNGLTSAQLTAYAVLSTLVKASYGRNDSLLSTRIRSGEISSDLLKPFHFPLMYL
                     CDTIGASLFQLFSRALPLLVFCVFVFDIRLDMHAETFAKFIPLYLLSFLLFFLMSFLI
                     SSMAFYFTDIFPFWIFYYALITLTSGAIIPVDFFPEEMQKLLYSTPFPYLFYFPTMAI
                     LGRDLVFSYPELVLRYLVLIGSALLLSWMSYRSGIRKLTIAGG"
     gene            complement(2673..3608)
                     /locus_tag="PLG78_11670"
     CDS             complement(2673..3608)
                     /locus_tag="PLG78_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE23820.1"
                     /translation="MGSVVSLFSAQKETITAVDHVNFDIGQGELVGYLGPNGAGKSTT
                     IKMLTGILVPTSGRVLVDGIDPEKDRRAVARKIGVVFGQKTQLWWDLPVEESFDLLRA
                     VYRIPGAEYKKRLNFFIELLDMKDFLSQQARKLSLGQRMRADLAASLLHRPPILFLDE
                     PTIGLDILTRDIVRKFIKEINEQEKVTILLTTHDIQDIEYLARRLILLDRGRVHYDGG
                     IDDFIQRYAAEKVLSLQVDPPLQSSFFTQRGFEVLSESSDRNFEIRLGADETPAALIE
                     ALSASKIRIDEINMRKRDLGDTLKLMYTNQRNVEL"
     gene            3748..>5074
                     /locus_tag="PLG78_11675"
     CDS             3748..>5074
                     /locus_tag="PLG78_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNE23821.1"
                     /translation="MPRAIAAMDLGTNSFHLAVVQPDDQGRFEILSTEKEAVRLGSGG
                     GDLNLILPDAMERGLTVLKRFTAISRSMNADIRAVATSAVREAKNSAEFLERVKKECG
                     LDVEVIPGKEEARLIYLGILQCLPVYDKRVLTIDIGGGSTEYLVGKSGIPEFAVSLKL
                     GAIRLKDRFFPGGVVTDKAVSECRQFVRIALTGARDETKSRGYEMAIGSSGTIETLWD
                     MIRLRKKIPELAEPVIHLQDLDRVVDDILSIEGPEKRAKLDGLDEKRADIIVGGAILI
                     QESARLLGISALYFSRYALREGVVFDALQRKSDHALGIPDVRRSSVDRLAESFARVTQ
                     VVHAPGVVNVAARLFDEVTATRPGIGTLEDRFLLECAAKLHNVGVAISHGSHHKHSYY
                     IIRNSDLLGFSPIEIELIALVARYHRKGEPKQNHPEYGSLAKSTQSRVYN"
BASE COUNT         1415 a         1265 c         1259 g         1135 t
ORIGIN      
        1 atgaattgat tgtaccgttc tagcatcgca gccacacgag tctgctgcag catgatctca
       61 gaaacccaga ttctgtaggg atcacgcgtc tctctgaagg gaagtttgcg atgctccctg
      121 gcataccatt gatacagcgg attcagatca ggcttagatt ttctcactga gataatccag
      181 agattctttt gcgatctgca aactctgctt gagtaagtcg agaggaggcc gacccagagg
      241 ggtattggct cgcgcatctt cgagagattc ctctgaagcc gactccatca aacgaaccac
      301 cccgggatct ggcgagggcc acccggcacc gtaagacgca gtgcgattgt agggacagac
      361 ttcctggcac acgtcgcagc cgaacatcca accctgcacg tgcggacgca gttcatcggg
      421 catggggctt cttctttcaa tggtcagata cgatatgcat tttcgcgcgt cgattttgta
      481 ttcagtcagt gcgccggttg gacatgcatc tatgcaaatt ctgcaagaac cgcatcgatc
      541 cagggcagga acacccggtt tcaattcctg tccgatgagc aaaacgctga tgaagaaaaa
      601 agatcccctg cccggatgaa tcagattcgt attctttccc tgaaagccaa gtccagcgag
      661 ccgtccaagc actctctctg caagaggagc cgtatcaatg cagaagcgac agcgaaggcc
      721 gggcaatttc tgtttcaagg cgcggccaag tttgcgacct ctctctcgga gcaagtcatg
      781 atactctccg gtgtgcgcat aacgtgcgat ctttactctg gcatgtttca gtgctgcatc
      841 cgattcgggc gtgcgataaa aggaaccaag aacgagagca ctctttgcat cgtgcatgag
      901 actgcgtgga tccaggcgca tttccagatt gcgcgcaaga tactccatgg tcccgtgtct
      961 gccttctgcc acaaaatctt tgaatgcagt ggtgtctttt tctggcagag aaatcggagc
     1021 aatggcggcc agatcaaagc caagagacag agcggtctct atgaatgtct gctcaactgc
     1081 ctgtgcttgc atatcgcact acggctcgtc gccagaacac aatcgcaatg atcatgcaca
     1141 caaggcccac tggaaaagtg aagtatacga atgccgggtg aaacaaagaa tccgcgctct
     1201 tattcaaaaa ataatgcgcc gggtaaaagt tgatgaaagc gagaggaaat aaaaacgtaa
     1261 gagcgaatcg aaccgggcga ttgtaaatat ccacaggata gagaagaaac tcccgcgagg
     1321 aaaatacaaa cagatgctgc attccttcat tggatatagt ataaaacgca gtggcggcaa
     1381 tgatgatgcg aatggccccc ataatcatca ctccgccgga cagcaccaga accagcatga
     1441 gaatggcctc tggcgaccac tggatatgaa ccagtctgtc tgctgtgaca aaggccacca
     1501 ctccaagcac aatgtttgcc aggccgcccg gctcaaatcg catggccaga atggaaaaca
     1561 gcggcgaaag tggtctaagt aaaactcgat caaacgttcc ctgcctgaca aagtctgaaa
     1621 attcaagaaa tccagagaac aatgcagata caactccagt tgcgatcaca agcaaactgt
     1681 aaagaaacgc aacttctccg gcattccagg tgccgatctt gttaaactta ttcaccatca
     1741 cccagagcac accaatttgc gcagagtaaa aacccatgat ggtaagaatg tacatgataa
     1801 aactaccgcg atattccatt ctactctgga tcgcagcgcg aatcatcgaa ccatatacag
     1861 gaagatcatt ccagaatttc ttcattttca accgcctgcg atggtaagtt tgcggatgcc
     1921 acttctataa gacatccacg ataacaagag agcacttcca atcaagacca gataacgtag
     1981 aacgagttcc ggataactga agaccagatc tcttcccaga atagccatgg ttgggaaata
     2041 aaacagatat gggaaaggag tgctataaag tagtttctgc atttcttccg gaaaaaaatc
     2101 cactggaatg attgcgccac tcgtcagtgt gatcaatgcg tagtaaaaaa tccagaacgg
     2161 aaagatatcc gtaaaataga atgccatgga gctgatcaaa aaactcatca gaaaaaacaa
     2221 cagaaaactc aacaaataga gcgggatgaa tttggcgaat gtctctgcgt gcatgtccag
     2281 gcgaatatca aatacaaaga cacagaatac cagaagtgga agggccctgg aaaatagctg
     2341 gaagaggctc gcgccgattg tgtcgcacag atacatcaga gggaaatgaa acggcttgag
     2401 caaatctgag gaaatttctc cgctgcgaat cctggtagaa agaagggagt catttctacc
     2461 gtagcttgcc ttcaccagag tggaaagaac agcgtaggcc gtgagctgtg cggaagtcag
     2521 accattcttc gtgctaccgt ccggaacgag tgtgcgccag acagaagtga aaatgaagat
     2581 gtacagaaag gcgttgagaa cagccatgaa atattccacg cgatagctga tagcgcgctg
     2641 aaaggacatg gaaataaact tgaaataagc cattagagtt ctacgttcct ctgattggtg
     2701 tacatgagct tgagcgtgtc tcccagatcc cgctttctca tattgatttc atcaattcga
     2761 atcttgcttg cactcagcgc ctcaatgagt gctgccggtg tttcatctgc gcccagcctg
     2821 atttcaaaat tgcgatcgga ggattctgaa agcacttcaa atcctctctg ggtaaagaag
     2881 ctactctgca aaggaggatc cacctgaagg gacagcacct tctctgccgc ataacgttga
     2941 atgaaatcgt ctattcctcc gtcgtagtga actcgtccgc gatccagaag gatcaaacgc
     3001 ctggccagat attcaatgtc ctgaatgtca tgagtggtga gaagaatcgt taccttttcc
     3061 tgttcattga tttctttgat gaattttctg acgatgtcac gtgtgagaat gtccagcccg
     3121 atcgtaggct catccagaaa cagaataggt ggacgatgca ggagcgaagc tgcgagatcc
     3181 gctcgcatac gctggcccag actgagcttg cgcgcctgct gcgaaaggaa gtctttcata
     3241 tccagcagtt caataaagaa gttcaaacgc ttcttgtatt ccgcgcccgg aatgcgatac
     3301 acagcgcgaa gtagatcaaa actttcctcc acaggaaggt cccaccacaa ttgcgtcttc
     3361 tgaccaaaca caacccctat ctttcgcgca accgcccttc tgtctttttc aggatcaatg
     3421 ccgtctacca gaacacggcc tgaagtgggc acgaggatgc cagtgagcat tttgatggta
     3481 gtagattttc cagcgccatt tggcccgaga taaccaacca gttccccctg accgatgtca
     3541 aagttcacat ggtccactgc cgtgatggtt tccttctgcg ctgagaacag agagacgacg
     3601 gaccccataa acccgggcct tctcttcctg gacgtaaatg attttgttag ttcttgaacc
     3661 tggatcacgc accagaatca aatcgataac cgcacgcgcg acagcaaaaa ttgtttccgt
     3721 caccaccagc caggctcttc tgtgcagatg ccgcgagcca tagctgccat ggaccttggg
     3781 accaactcct tccaccttgc agtcgttcaa ccagacgatc agggccgctt cgaaattcta
     3841 tccactgaaa aagaagcggt tcgcctgggg tcaggcgggg gagacctcaa tctgatttta
     3901 ccggacgcga tggaaagagg tttgaccgtg ctcaaacggt ttaccgccat ctccaggtcc
     3961 atgaatgcgg acattcgcgc cgtggccact tccgctgtgc gcgaagcaaa aaacagcgcc
     4021 gagtttctag agagagtcaa gaaagaatgc ggcctggacg tggaggtgat tcctggaaag
     4081 gaagaagcgc gcctcatcta tcttggaatc ttacagtgcc tgcccgtgta tgacaaaaga
     4141 gtactgacga tcgacatagg gggcgggtct acagaatacc tggtcggcaa aagtgggatc
     4201 ccggaattcg cggtatctct gaagctcggg gccattcgcc tcaaggatcg attcttcccc
     4261 ggaggagtcg taacggacaa agctgtttct gaatgcaggc agtttgttcg cattgcactg
     4321 accggagcgc gagatgaaac caaatcgcgt ggatacgaaa tggccatagg aagttcggga
     4381 actatagaaa ctctctggga tatgatccga ctgcgaaaga agattccgga actggcagag
     4441 cccgtcattc atctgcagga tctggatcga gtggtagacg acattctttc cattgagggc
     4501 ccggaaaagc gcgcaaagct agatggactg gatgaaaaac gcgccgacat catcgtgggt
     4561 ggtgcaattc tgattcagga atcggcaagg cttcttggaa tttcagcgct ctatttctcc
     4621 agatatgctt tgcgcgaggg tgttgttttt gatgcgctgc agcgcaaatc ggatcatgca
     4681 ctgggaattc cagacgttag gcgatcttct gtggatcgtc ttgcggaaag ttttgcaaga
     4741 gtcactcagg tggttcatgc tcctggagtt gtaaatgtag ccgcacgcct gtttgacgaa
     4801 gtgaccgcaa ccagacctgg aattggcacg ctcgaggaca gatttctact cgaatgcgca
     4861 gccaaactgc acaacgtggg cgtggcaatt tcgcacggat cgcaccacaa acatagctac
     4921 tacatcatac gaaacagcga cctgcttggc ttctcgccca ttgaaatcga actcattgct
     4981 ctggtggcgc gctatcacag aaagggtgag cccaaacaaa accatcctga atacggatcc
     5041 ctggcgaaga gcacgcaaag cagagtttat aatc
//
LOCUS       DAOQJL010000262         5073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9662_length_5073_cov_4.570546, whole genome shotgun sequence.
ACCESSION   DAOQJL010000262 DAOQJL010000000
VERSION     DAOQJL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9662_length_5073_cov_4.570546"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PLG78_11680"
     CDS             complement(<1..1426)
                     /locus_tag="PLG78_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23822.1"
                     /translation="MIRASAFFLLFFATSLYTQPSQNRLEALYPGISSQIDRIGKLEP
                     DLASPSNRSALADYLQSNDPELRKGMKLILESELQSTMTRISSKLVVDGSDQDWKDVP
                     FVVTDPAGDNAADILEQRVFLDESGNLFIMMKPSLKAGAIPPEYIFQPLIYANGDRGS
                     FLKIYFAKGRARISERTGDKPEDLEIPGVEAAVGTVYEVKVPLDAWMRSKGGVGSLDV
                     WTGYFDRDKYDSVSQSIPILQTNYALLLLLDLIARGAVAGHDPITVALAYANSTLYAM
                     GDLDTRAQIRKDGREHFQLYRKVSAWQKSNSLPFELENLPVIPKMIWSARYNRLHHFG
                     LMREAIGLLGKKRLTKDIYLEFVDRPEVLEKIHDEIVARDLPLSRDITVLASNIEDLG
                     SSLCETRFALWKYEDLYKRGKMSKEDYDRIQAEAKNNYEIEYMGKKRNWEEFRWNNYQ
                     FQNRCFRGSKILGDCGTYTQAMTAI"
     gene            complement(1642..2568)
                     /locus_tag="PLG78_11685"
     CDS             complement(1642..2568)
                     /locus_tag="PLG78_11685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE23823.1"
                     /translation="MLQYKLKSMSDWSDEAEPLASDLAAQILEGTKEGIIITDAESRI
                     VWVNPAFTSITQYTASECLGRRPSILASGSHGREFYEDMWRELSATGAWTGEIWNRKK
                     DGTIYPEWLSITRVSFRGEDRYVGIFSDISRRKAAEARIQYLAHFDALTGLPNRHFLV
                     DRANQVMSLCRRRRLPLSLMLLDLDYFKTINDKLGHSTGDEALKAVASRIQSIIRHED
                     TVARLGGDEYVILLPETNQDGAFHVASKILKMVTEPLRTGSELVHLSLSIGVAVFPEH
                     GASFEILCQNADAAMYEAKRAGGNKFRFFTLR"
     gene            2596..4491
                     /locus_tag="PLG78_11690"
     CDS             2596..4491
                     /locus_tag="PLG78_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23824.1"
                     /translation="MSPASCETSDRITAKNTLAYGEGHLNGTSRAFIARYSILLCFLC
                     SGIEAANPIRLNSLVGRTYIGERLEEFEDTSGSLAFADVQSQTFHNVGSKTVHHGFTD
                     SVWWFRFSYDCAGVPEDQVYFLSMFNFRMYDASIYVATDGSLVRQMQSGMIGKRDVRT
                     TDIAFRLPRTKNAATVYLRVKGITPVRFAPSIQSEEELRNNQLTSDMLIGLAMGGQFS
                     LAIFNLVLFAAIRDRSYLSYGVFQILTVLLVLNGSGYTYEWFLSGYPMLDLSLTAPLG
                     MLTSVAIILFSADFLHIGKGLPRSVIRYFIAGLALFALLSFVIPLRVSIRYSSYFAVA
                     VCVLVFWLAIIRHRAGFGPARFFFVAFLPLLAFAFTFVISRFLTFIKTGPFSLHIFAA
                     GLPLAILLSSVLFSVALSDRFQTLRNALRKTMAAKDELRMRNEQALREEKHRINRELH
                     DVIGSDLSALIFELRKDHEHDSILPRLSRMLSRLRDLVHINALDPDFDLSSQIRSFLR
                     EIEMPGVRVEASIDEVALPVVSAFHLHRIFLESITNCLRHANATTLIIRLKRRHDKVT
                     LIVADNGQGFDVNKPGNGMTGVRERAQTIGAHLRIVSRSGKGTAVVLHISEAGAFLPL
                     DARGVLH"
     gene            4500..>5073
                     /locus_tag="PLG78_11695"
     CDS             4500..>5073
                     /locus_tag="PLG78_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE23825.1"
                     /translation="MAIRILILEDDPNITRTLAEMFASNSDFQLLGAYSTGADFFRDV
                     HKAADVLLLDFELPDQNALSVLRRLKSSEALRDMKVLIFTAFEDETRLIHAMEAGANG
                     FLLKDTSPELLLAEIRSVHLGGAPITARMARALLGHFAPPSTRADGLLTQQERRVLQL
                     LSMGLTYAEAAHKLGISINTLKNYTENTYRK"
BASE COUNT         1231 a         1310 c         1219 g         1313 t
ORIGIN      
        1 agatcgcagt catagcctga gtgtaagtgc cgcaatcgcc taaaattttg gaaccgcgaa
       61 aacaacgatt ctggaattga taattattcc atctaaactc ttcccagttc ctcttctttc
      121 ccatatattc gatttcataa ttgttttttg cctctgcctg gatgcgatcg taatcctctt
      181 tgctcatctt tcccctcttg tagaggtcct cgtatttcca gagcgcaaag cgagtttcgc
      241 acagggaaga cccaagatct tctatgttcg atgcaagcac tgtaatatcg cgcgaaagag
      301 gcaggtcacg cgcaacaatt tcgtcatgga tcttctcaag gacctcaggc ctgtccacaa
      361 actcgaggta gatgtccttt gttaggcgtt tcttgcccag aagaccaatt gcttcacgca
      421 tcagtccaaa atggtgcaga cgattgtaac gtgcgctcca gatcatcttg ggtataacag
      481 gcaagttttc cagctcaaaa ggaagcgaat tggatttctg ccaggcggac accttacgat
      541 acaactggaa atgctcacgt ccgtctttgc gaatttgagc tctcgtgtcc agatctccca
      601 tggcgtataa cgtgctattc gcgtatgcga gagccaccgt gattggatcg tgacctgcaa
      661 cagcacctcg tgcaatcaga tcgagtaaca gcagtaaggc gtagttcgtc tgaagaatcg
      721 gaatcgactg tgaaacagaa tcatacttat cgcggtcaaa atatccggtc cacacgtcga
      781 gagaacccac gccgcccttg cttcgcatcc acgcatcgag aggaacctta acttcataaa
      841 ctgtgccgac cgctgcctct acaccaggaa tctccagatc ttctggttta tctcccgtcc
      901 gctcgcttat tcgtgctctg ccctttgcga aataaatctt caagaaggag ccgcggtctc
      961 cattggcgta aatgagaggt tgaaaaatgt actccggtgg gatggccccg gccttcaggg
     1021 aaggtttcat catgatgaac aagtttcctg attcatcgag gaagaccctc tgctccagga
     1081 tgtcggctgc attgtctccg gccgggtccg tcacaacgaa cggcacatct ttccagtcct
     1141 gatcggatcc atctactaca agcttgcttg agattcgagt catggtagac tggagctccg
     1201 attccagaat cagtttcatt cccttgcgta attccggatc gttcgactgt aaataatccg
     1261 cgagagcact gcgattggag ggactcgcca gatcaggctc cagtttccca attcgatcta
     1321 tctgcgaaga aatacccgga taaagggcct ccagacgatt ctgtgaaggc tgcgtataga
     1381 gagatgtcgc aaaaaacaaa agaaagaagg cactggcgcg gatcatcgct tttcctctga
     1441 gacgtggata cgaccagagg ggttctctct tttgttctcg caaggactta acgttgcgtc
     1501 agaggacaac aatcgtcatg ggttttgctg tctcgtcgag tacaacacga tttcgcgtgg
     1561 ttctaaggaa gattcgaacc gaggttacag aacgacccag gtgctttatg atcaagcgaa
     1621 aggggaaaga tttccccctg gtcatcgtaa agtaaagaat ctaaacttat tcccacctgc
     1681 tcgctttgct tcatacatgg ctgcatccgc attctgacat agaatctcga acgaggcccc
     1741 atgttcggga aagacagcaa ctccaataga caacgacagg tgcacaagct cggagccggt
     1801 tcgcaatggt tccgtgacca ttttcaatat cttgctggcc acatgaaacg caccgtcttg
     1861 atttgtttcc ggaagcaaaa tgacgtattc atcgccacca agtcgagcca ccgtatcttc
     1921 atggcgaatg atggattgaa ttctagacgc gacggctttg agtgcttcat cgcctgtgct
     1981 atgtcctaac ttatcgttga tggtcttaaa ataatccaga tcaagaagca tcaaagaaag
     2041 aggaagacga cgcctgcggc agagagacat gacttgattg gctcgatcta ccaggaaatg
     2101 acgattcggc aagccagtga gagcatcaaa atgagccaga tattgaatgc gagcctccgc
     2161 tgctttgcgc cgcgaaatgt cggaaaagat tccaacataa cggtcttcac cgcgaaaact
     2221 cacgcgcgtg atggagagcc attccggata aatcgtgccg tccttcttcc tattccagat
     2281 ctctccagtc caggcgccag tcgcgctcag ctctctccac atgtcttcgt agaattcacg
     2341 accgtgcgaa ccggatgcca gtatggaagg tcttctaccg agacactcag atgcagtgta
     2401 ttgagtgatg cttgtaaatg cgggattcac ccagacgatg cgactctccg cgtccgtgat
     2461 gatgatgcct tccttggtac cttcgaggat ctgcgccgcc agatctgacg ccagaggttc
     2521 ggcttcatcg ctccaatcac tcatcgactt cagtttgtac tgtaacacat gggctcatcg
     2581 cacgcaaggg ggagaatgtc ccctgcatcc tgcgaaacga gtgacagaat cacagcaaaa
     2641 aacactctcg cctatggcga gggccatttg aacggaacct ctcgcgcttt catcgcgcga
     2701 tattcgattc ttctctgttt cctctgttcg gggatagaag ctgcaaaccc cattcgcctg
     2761 aactcgctcg tgggacgcac ctacattgga gaacgtctgg aggaattcga agatacgagc
     2821 ggctccctgg ctttcgcaga tgtacagagc cagacatttc acaatgttgg ttcaaagacc
     2881 gtccatcatg gcttcacaga cagcgtctgg tggttccgct tttcatatga ttgcgcggga
     2941 gttccagaag atcaggtata ttttctgagt atgttcaatt tcagaatgta cgatgcctct
     3001 atctacgtcg caacagacgg gtctctcgtc agacaaatgc agtcgggaat gatcgggaaa
     3061 cgcgatgtaa gaacaactga catcgccttt cgccttcctc gcacgaagaa cgcagccacg
     3121 gtttatcttc gcgtaaaagg aatcacacct gttcgattcg caccatcgat tcagtcagaa
     3181 gaagagttgc gtaacaatca gctcacttct gatatgttga ttggcctggc catgggcggt
     3241 cagttctctc tggcaatctt caatctcgtg ctcttcgcgg ccattcgtga tcgtagctat
     3301 ctttcctacg gagtctttca aatcctcacg gtattactcg tgctcaacgg atccgggtat
     3361 acgtatgagt ggtttctaag cggttacccg atgctggatc tttctctaac ggcgcccctg
     3421 ggaatgttga ccagcgtcgc gatcatactc ttcagcgctg actttcttca catcggcaag
     3481 ggcttaccca gaagtgtgat tcggtatttc atcgctggac ttgcgttgtt cgcacttctg
     3541 tcctttgtca taccactgcg tgtgtcgatc cgctattcgt cttactttgc agttgccgtt
     3601 tgtgtgttgg tgttctggct cgcgatcatt cgtcaccggg ccggttttgg gccagcgaga
     3661 ttcttttttg tagcgtttct tccacttctt gcattcgcat ttacgttcgt cattagccga
     3721 tttttgacct tcataaagac gggcccattc tctctccata tctttgccgc tggcctgcct
     3781 ctggcgatcc tgctgagtag cgttttgttt tcagtggcgc tctctgatcg attccagact
     3841 cttcgcaacg ctcttcgcaa aacgatggca gcgaaggatg agcttcgcat gcgaaatgag
     3901 caggcgctga gagaagaaaa acaccgaatc aaccgggaac tgcacgatgt aattggatca
     3961 gatctttccg cactcatttt cgaattacgg aaagatcacg agcacgacag catcctgccg
     4021 agattgagca gaatgctttc acggttgaga gatctggttc atatcaacgc actcgatcca
     4081 gactttgatc tttcgtcgca gattcgctct tttctaagag aaattgaaat gccaggagtg
     4141 cgtgtggaag cttctatcga tgaagtggcg cttccagtgg tcagcgcatt tcatctgcat
     4201 cgcatttttc ttgagagcat tactaattgt ttgaggcatg cgaatgcaac cacccttatc
     4261 attcgcctga aacgcagaca cgataaagta acactcattg ttgctgacaa tggccagggg
     4321 tttgatgtga ataagccggg caacgggatg actggagtac gcgaacgagc gcaaacgatt
     4381 ggcgcgcacc ttcgcattgt ttcacggtcg ggcaaaggca cggcggtagt gctccatatt
     4441 tcggaagcgg gggcatttct cccccttgac gcgaggggcg tcctgcatta agaataggta
     4501 tggcgattcg aatcctgata ctcgaagatg atccaaacat caccaggact ctcgcagaga
     4561 tgttcgcctc gaattcggat ttccaacttc taggagctta ttccacagga gcagatttct
     4621 ttcgcgacgt tcataaagcg gcagacgtgc tcttgctaga ttttgagctt ccggatcaaa
     4681 acgcattgtc tgttcttcgt cgtctgaaat ccagcgaggc acttcgcgat atgaaggtgc
     4741 ttatcttcac cgcatttgaa gatgaaacgc gcttgattca tgccatggaa gccggcgcaa
     4801 acggatttct tttaaaagac acttcccccg aactgttact cgctgaaatc cgaagtgttc
     4861 atctgggcgg agcaccgatc accgcgcgaa tggcaagagc acttctgggt catttcgcac
     4921 caccatcgac gagggcagac ggactcttga cgcaacagga acgccgtgtg ttgcagctct
     4981 tgagcatggg acttacctac gcggaagccg cgcataagct gggaatatcc atcaatactc
     5041 tgaaaaacta tacagaaaac acgtaccgta aac
//
LOCUS       DAOQJL010000263         5035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9779_length_5035_cov_3.784137, whole genome shotgun sequence.
ACCESSION   DAOQJL010000263 DAOQJL010000000
VERSION     DAOQJL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9779_length_5035_cov_3.784137"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLG78_11700"
     CDS             <1..679
                     /locus_tag="PLG78_11700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE23826.1"
                     /translation="RIDHVMALCRLYWVVSTSAGKTGAYVEYPFVELLRLAALVSQRK
                     RCMVIGEDLGTVPGYFRDALSRAELFSWKILFFERDGENFKDPGSYPARSIATLNTHD
                     LPTFLGWMEGEDIRIREALGLVTSFESEKAARERRLDIARARKFAGSTEGESSQDLCA
                     KLYGRLLNSSSAIRLVSIYDLFGEEEQPNLPGTTDEYPCWSMRHRLELSALGSMPLFS
                     SLFPVRS"
     gene            complement(792..1091)
                     /locus_tag="PLG78_11705"
     CDS             complement(792..1091)
                     /locus_tag="PLG78_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23827.1"
                     /translation="MAQGDSADQKAQRLDHLVTVYEKIARLTEQELANAYEIIKMYEA
                     INEYTRKELIQVKKTNQAQEAVSELGSEELKKSFDRITQLEEANKKLQEERSRIQ"
     gene            1173..2087
                     /locus_tag="PLG78_11710"
     CDS             1173..2087
                     /locus_tag="PLG78_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE23828.1"
                     /translation="MIEVKEVSRTFGDFTAVQGVSFNVDRKAGITGLLGPNGAGKTTT
                     MRMITGYLRPSSGDILIDGESVRDEENLTGIKRRIGYLPETTPLYPEMLVSEFLKFMG
                     EVRGVHGAELDASAVQMVDRLDLGSHLYSPIGILSKGFRQRVALAGTLIHKPEVIILD
                     EPTSGLDPNQISEIRSLIVELGKTATVILSTHILQEVEELCNRVIIINRGRVVADETT
                     AVLKSVQACSVVARGEDVEKKLASIPIVTSVIAGQNHKDGFRQYLCEVGENGPEKLFA
                     ELAGKSLEVREFAPVARSLQSVFEELTN"
     gene            2084..2815
                     /locus_tag="PLG78_11715"
     CDS             2084..2815
                     /locus_tag="PLG78_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE23829.1"
                     /translation="MKFLFRRELSNYFNTPIGYVFAVACLFFNFVFFFAGIWAIPDLF
                     PAFWDARSASIRSYMNLLPLTFILMVPAVSMRIWAEERKSGTLELISTLPLREVDLVI
                     AKFLGAWVYVGLLIVASLPLAITIWFMGHLDWGSTLALYAGSILMAGAYVSTGMVVSA
                     LTREQIVAFIITFFISLYMFISNYYIVSQHLSPGAARLIGFFSHSHHFTTFSRGLLDA
                     ADVMYYVTFIGMMLTANVWILRRER"
     gene            2812..4545
                     /locus_tag="PLG78_11720"
     CDS             2812..4545
                     /locus_tag="PLG78_11720"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNE23830.1"
                     /translation="MKSFFSALNHNTLFIRVNTVLLFLLLNGAASSWNCRWDMSRDRV
                     NSVTESTEKVFARQRDPVLVEAYITSDLPGELKAMLSPILYQLDEIGRVGGKKVRLRI
                     INPDTEEKKQAAEQRGIQGNPFEQARVDEVSQRLGYFGIYIQSGEKSTVLPLVEEGRI
                     IEDFEYRFLREVKALNKKDGPSGIGYVRVRGTLDTRSFQQSQEPDKDNLFGFYTLMQR
                     AFGNIVDVDLSREVDRDVETLILTGLPHFDDRQAYHLDQFLMRGGNLVCMLKGFDFQL
                     QQMDPRMARMGIGGQGRAFAMVQQDELSHFNQLFSKYGISLRGEVLFEPELAAPEMDI
                     QGQYVQKVRNPSWAVYSRDTGTIVSDLPAVRGVQQVVFPWVSGFDLKEDKQPNVTFSP
                     LVVTTPDAISKDTAPLGLREMQKIGRDPSDVRVDHSLPVAVLASGRFQSAFSEKDVPP
                     GEDKASYRGAQAGSRRSSIIVLGSAYMVSDILLRNESNFQIFRINQAFVTNLVEAAQG
                     DNDLAAARARVGGIDYLRPLFSRDDTLQAGFEKLFMWFHILFLPVLLGIYGARRLALR
                     GRRRGLEEVAK"
     gene            4542..>5035
                     /locus_tag="PLG78_11725"
     CDS             4542..>5035
                     /locus_tag="PLG78_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23831.1"
                     /translation="MNSIWSKIKSNPGLSLLSANVVLLAVIVLMQDPFGLFKTGYAGA
                     RVLLSASQDSISTIEVESPDKPGILRITRKDKLPVKKGEAPEYTWDVERTDGTKTTRM
                     SADRDRVKELFASLEKTRRYYGIARNSESEKDAEMATDSKGRLQCLQVRLISANGKKN
                     TLYVG"
BASE COUNT         1165 a         1205 c         1331 g         1334 t
ORIGIN      
        1 gcgaatcgat cacgtgatgg ctctctgtcg cctgtactgg gtagtatcca cttctgcagg
       61 aaaaacaggt gcttatgtag agtatccgtt tgtagaactg ctgagactgg cagccctcgt
      121 cagccagaga aagagatgca tggtgattgg tgaagacctg ggaactgtgc ctggctactt
      181 tcgcgatgcg ctcagtcgcg cagaactctt ctcctggaaa attctatttt ttgaaagaga
      241 cggtgagaac tttaaggatc cgggctcgta tcctgctcgt tccattgcca ctctcaatac
      301 gcacgacctg cctacatttc taggctggat ggaaggagaa gacattcgca ttcgtgaagc
      361 gctgggcctg gttacttctt ttgaatctga gaaggcagcg cgcgaaaggc gcctggacat
      421 tgcgcgggcc cgaaagttcg ctggatccac ggaaggggag agcagtcagg atctatgtgc
      481 gaagctctac ggtcggcttc tgaattcttc ttctgccatt cgcctggttt ccatctacga
      541 cctgtttggc gaagaagagc agcccaattt accggggaca acagacgagt atccttgctg
      601 gtctatgcgt cacaggctgg agttgtccgc tctcggctct atgcctttgt tttcttcctt
      661 gtttccagtg cgatcttgag tcctggagat cccgcgagag gtaagccagg cctggcgtcc
      721 ggcaaacatt cttttatctg gtgtcttgtc tgatctatca cataacggcg cgtaggtgct
      781 gacggaccgg atcactggat ccggcttctt tcttcctgga gtttcttgtt tgcctcttct
      841 agctgggtga tccggtcaaa agactttttc agctcttcgc ttccgagttc cgacacagct
      901 tcctgggcct ggttggtttt tttgacctgt atgagttcct tgcgcgtata ctcattgatg
      961 gcttcataca tcttgatgat ttcgtaggcg ttcgccagtt cctgctctgt gagccttgcg
     1021 atcttctcgt agactgtgac gagatgatcg agacgctgtg ctttttgatc tgcggaatca
     1081 ccctgggcca tgaggcaaag gacgctcgtt ttgtgggatc gtcaaaacaa ttttaaattg
     1141 cgccgaaggg gcgtgcggag ggttttgttc ccgtgattga agtaaaagaa gtgagccgga
     1201 cgttcggcga ctttacagca gtccagggcg tcagtttcaa tgtggaccgc aaagcgggca
     1261 tcacgggact ccttggacca aacggcgccg gcaaaacgac caccatgcgc atgattacag
     1321 gttatcttcg ccccagcagc ggcgatatcc tcatcgacgg cgaatcagtg cgcgatgaag
     1381 aaaatctaac cggaatcaaa aggcgaatcg gttaccttcc ggaaacaact cctctctatc
     1441 cggagatgct tgtatctgag tttttgaaat tcatggggga agttcgcggc gtgcacggag
     1501 cagagctaga cgcttccgcg gtccagatgg tagatcggct cgatctggga agtcatctct
     1561 attcgcccat tggcattcta tcgaaagggt ttcgtcagag agtggcactt gcgggaaccc
     1621 tgattcacaa accggaagtg atcatcctgg atgaaccaac atcgggtctc gatccaaacc
     1681 agatttcaga gatccggtct ttgatcgttg aacttggaaa aacggccacc gtgattcttt
     1741 ccactcacat ccttcaggaa gtagaggaac tctgcaatcg cgtgatcatc atcaatcgag
     1801 gcagagtggt agcagacgaa accacagcag ttctcaaaag cgttcaggcc tgttctgttg
     1861 tagcacgcgg agaagatgtg gagaagaaac ttgcatccat tccaattgtg acttctgtaa
     1921 tcgcgggaca aaatcacaaa gatggctttc gtcaatattt gtgcgaagtg ggggagaacg
     1981 gtccagagaa actctttgca gagctggcgg gcaagtctct ggaagtcaga gagtttgcgc
     2041 ctgtggccag atcgcttcag tccgtctttg aggagctgac aaattgaaat tcttattcag
     2101 gcgtgaactg agcaactact tcaacactcc catcggctac gtgtttgcgg tcgcttgcct
     2161 gttttttaac tttgtatttt tcttcgcagg aatctgggcg attccggatt tgtttcctgc
     2221 attctgggac gcacgcagcg cttccatccg atcgtacatg aatctgcttc cgctcacatt
     2281 tatactcatg gttccggcag tgagcatgcg catctgggca gaggaaagaa aatcaggaac
     2341 tctagagttg atctccactc ttcctctccg ggaagtggat ctggtcatcg cgaaattcct
     2401 gggagcctgg gtctacgttg gactgttgat cgtcgcttct cttccgcttg ccattaccat
     2461 ctggtttatg ggtcatctgg actggggtag cactctggca ctctatgccg gctccattct
     2521 catggcgggc gcatacgtaa gcaccggaat ggttgtgtcc gctctcacgc gtgaacagat
     2581 cgttgctttc atcattacat tctttatcag tctatacatg ttcatttcca actactacat
     2641 tgtatctcag catctcagcc ctggtgcagc aagactcatc gggtttttct ctcacagcca
     2701 tcactttaca actttttctc gtggcctgct ggatgcggct gatgttatgt attacgtaac
     2761 atttataggc atgatgctca cggcgaatgt gtggattctc aggagggaga gatgaaatct
     2821 tttttttccg ctctcaatca caacacattg ttcattcgcg tcaatactgt gcttttgttt
     2881 ttgcttttga atggagccgc cagttcctgg aattgcagat gggacatgag tcgcgatcga
     2941 gtgaattctg tgacagaaag cacggagaaa gtcttcgcac ggcagcgcga tcccgtactt
     3001 gttgaagcat acatcacctc tgatttgcct ggtgaactca aggcaatgct cagccccatt
     3061 ctctatcagc tggacgaaat cgggcgagtg ggtggaaaga aggtccgcct tcgcatcatc
     3121 aatccggata cagaggaaaa aaagcaggca gcagaacagc gcggcattca aggaaaccca
     3181 tttgagcagg cgcgtgtgga tgaagtgagc cagaggctag gctattttgg aatctacatt
     3241 cagtctggag agaaatccac agtgcttcct cttgtagaag agggaaggat cattgaggat
     3301 tttgaatatc gattcttgcg cgaagtcaag gctctcaata aaaaggacgg accatccgga
     3361 atcggctatg tgcgcgtacg tggaacgctc gacacgagaa gtttccagca atcgcaggag
     3421 ccggacaaag acaatctgtt cgggttttac actctgatgc agcgcgcctt cgggaacatt
     3481 gtggatgtgg atctttcgcg agaagtagat cgcgatgtgg aaactctcat tcttacaggt
     3541 ttgcctcatt tcgacgatcg ccaggcgtat cacctggatc agttcctcat gcgcggtgga
     3601 aacctggtgt gcatgctcaa gggatttgat tttcagctgc agcaaatgga tccgcgcatg
     3661 gctcgcatgg gaatcggagg tcagggccgc gcctttgcca tggtgcagca ggatgagctt
     3721 tcgcatttca atcaattgtt ttcgaaatat ggcatatcgc ttcgcggaga agttcttttt
     3781 gaaccagagc tggctgcgcc tgaaatggac attcagggcc agtatgttca gaaagtgaga
     3841 aatccgtcct gggctgtgta ctcgcgcgat accggaacca ttgtatctga tcttcccgcc
     3901 gtgcgcggag tgcagcaggt tgtgtttccc tgggtttctg gttttgatct gaaagaagac
     3961 aagcagccaa acgtaacttt ctctcctctc gtcgtcacta ctccggatgc aatttcaaag
     4021 gacacagctc cccttggtct tagagagatg caaaaaattg gtcgcgatcc ttctgatgtt
     4081 cgagtggatc attctcttcc agtggcagtg cttgcatccg gtcgttttca aagtgcattt
     4141 tctgaaaagg acgtgcctcc aggagaggac aaggcatcct atagaggggc acaggcagga
     4201 agcagacgat cgtctatcat tgttcttgga tctgcttaca tggtatctga cattctgctt
     4261 cgcaacgagt cgaactttca gattttcaga atcaatcagg cattcgtaac caatctagta
     4321 gaagcagctc agggagacaa cgatctggca gctgctcgag cgcgcgtggg cggcattgat
     4381 tacctcagac ctctgttttc gcgcgatgat acgttgcagg ctgggtttga aaaactattc
     4441 atgtggtttc acatcctgtt tttacctgtg ctccttggta tttacggcgc ccggcgactg
     4501 gcactccgcg gtcgtagaag aggactggaa gaggttgcga aatgaattcc atctggtcta
     4561 aaatcaagtc gaatcctggc ctttctctct tgtcggctaa cgttgtactt ctggcagtga
     4621 tcgtcttgat gcaggatcca ttcggtctat tcaagacagg ttatgccggt gcgcgagttc
     4681 tgttgtctgc ttcccaggac agcatttcta cgattgaagt ggagtctccg gacaaacctg
     4741 gcattcttcg catcactcgc aaggacaagc ttcccgtaaa aaaaggagag gctcctgaat
     4801 acacctggga tgtggagaga acggacggaa caaagaccac tcgaatgtcc gcggaccgcg
     4861 atcgagtcaa agaactcttt gcaagtctgg aaaagactcg tcgctattat ggaatcgcac
     4921 gaaattctga aagcgaaaaa gacgcagaaa tggcaacgga cagcaagggc aggcttcagt
     4981 gtctgcaggt tcgactgatt tccgcaaacg gaaaaaagaa cactctatat gtggg
//
LOCUS       DAOQJL010000264         5023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9820_length_5023_cov_4.000805, whole genome shotgun sequence.
ACCESSION   DAOQJL010000264 DAOQJL010000000
VERSION     DAOQJL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9820_length_5023_cov_4.000805"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLG78_11730"
     CDS             complement(<1..538)
                     /locus_tag="PLG78_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23832.1"
                     /translation="MKTLALILVLFASQTFAQALEDNIVYREAVENARKSGFGDLEVD
                     NLHGEIAARVKAILEAGGLPEGPGNFEELTDGAKVRPVITGKDRAGKPYLRLRLRDGF
                     SIASTEFPSQFVFRAHCYLYPKEGGGLEKIVFQFYRINHSGSSYVRDMRRYIHPDPRD
                     QAQAAGKPIGAPLELLNNS"
     gene            673..1083
                     /locus_tag="PLG78_11735"
     CDS             673..1083
                     /locus_tag="PLG78_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE23833.1"
                     /translation="MHRAIPPIQKYMTTTPVTVQQNTTIAEAHKLMQEHNFRHLPVMK
                     GTEFFGVVSDRDLKLMESFTGVDPSKATVGDIAHKDAYTISPDAQTDDVVKTMATHKY
                     GSALIIDNKKVVGIFTTVDAMNAFAEVLEALLKK"
     gene            complement(1107..2312)
                     /locus_tag="PLG78_11740"
     CDS             complement(1107..2312)
                     /locus_tag="PLG78_11740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE23834.1"
                     /translation="MKPPGVFAALGFRNYRLWFAGQLGSLVGTWMQATAQAFLIYQLT
                     HSPAYLGYAGFASGAPAVLGLFGGVVSDRMPRRILLLITQTTMMILAAILAALVFAHL
                     VEPWHIIALSLGLGIANAFDAPARQSFVAELVDRDYLTNGIALNAIMFNLARAAGPAA
                     AGAVYGAFGAGFCFLVNAFSFLAVIVALLAIRTVASEKRKAESLRIEIAEGIGFAFGH
                     PVIRIILLIVAMVAIFGLGYITIIPAWTVTMLGGSALEIGLLQGAQGLGALLGAFAIA
                     AFARGSRGKMLLTGLLLFPLAILGFAMSRNVILSGLLLVAGGFFVMFVFNLANAIVQT
                     IVPDHLRGRVISVYTLCFLGTMPIGSIVAGAIAEWKGELFTVILGGVVLSVFAVSAWF
                     LARPLRARD"
     gene            complement(2309..3079)
                     /locus_tag="PLG78_11745"
     CDS             complement(2309..3079)
                     /locus_tag="PLG78_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE23835.1"
                     /translation="MEIKGATVVITGAAGGLGKAMSFAFADAGAVVVISDLREDSLAA
                     AVNEVEARGGKAIAVTADTSKEADAERLMKEAVSRTGRLDVAVLNAGILRDGLLVRVD
                     KETGKVKGKLSLDQWQAVIDVNLTGVFLTGREAAVQMIETKSKGVIIPIASVSMHGNP
                     GQTNYSAAKAGVAAMTKLWAQELSRFRIRSAGIAPGFIATDMVMKDMKPDALAKWESV
                     IPIGRLGKPEEIAASALFIAQNDLVNGVILEISGGVKV"
     gene            complement(3070..3546)
                     /gene="ybeY"
                     /locus_tag="PLG78_11750"
     CDS             complement(3070..3546)
                     /gene="ybeY"
                     /locus_tag="PLG78_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNE23836.1"
                     /translation="MHRIYRRVRIDVRDRIPSDRKIRTVLTQWLTHLGYKNAIIEISL
                     VSDPAIRRVNAEFRGKNKPTDVLSFPAQEFTRTRVPRLVSDWPQAGPLPLGDIMISIP
                     TARRQAREIGHSIQDEFYRLLSHGVLHLLGYDHERSRAEEKRMIAREDELTSFLWK"
     gene            complement(3546..>5023)
                     /locus_tag="PLG78_11755"
     CDS             complement(3546..>5023)
                     /locus_tag="PLG78_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE23837.1"
                     /translation="DIPHMRIAAGEPIVKQGDVIRQENRTALEVHARLHLREKAKRIT
                     AITLQHFLLLMILVFFAYRFESKRMSDMSANIIFFMNIWIFCGMLFAIETLWHEPNIN
                     PEDHIFGSWVPIGIFAVLFSLLSGELFALATAVYLSLLVFGVSQYDPQSFLHSLVMSV
                     MAVWAGTRIRKRIHFLAASIFLMLLAGVLVTMGYLYLNRVIISQSGGDLLTTGYKSAM
                     VKALLMGAATAFTIVILPLYESVFNVPTRFRLQELSDPSTPLLQEFYRLAPSTWNHTL
                     TVASLTEKACERLGLNTLLARTGVYYHDIGKTRRAGFFVENQHLIPKPENIDKNNPEK
                     AAKVIIDHVLDGIEIARKIRLPREVMAFIPEHHGTSTMSFFYHQALEKSRRKVKRENF
                     RYPGPIPQSKETAIVMIADSVEAASRSLDEITETAVDSLIQKIINIKLAENQFDESGL
                     TMGDLAVIRKAFTEILLSSLHHRPKYPAMEATKKLEQARKG"
BASE COUNT         1358 a         1319 c         1194 g         1152 t
ORIGIN      
        1 tgctgttgtt gagaagttca agaggcgcac caatcggttt gcctgcagcc tgcgcctgat
       61 cccttggatc aggatgaatg taacgacgca tgtcgcgcac gtaagaagaa ccggaatgat
      121 tgatgcgata aaattgaaat acgattttct cgaggccgcc gccctctttt ggatacagat
      181 agcaatgagc gcgaaacaca aactgagatg gaaattccgt agaagcaatg ctaaatccat
      241 cacgcagcct gagcctcagg taaggcttgc ctgcgcgatc ctttcccgta atgacaggac
      301 gcaccttcgc tccatccgtc agctcttcga aattgcccgg accctctggc agaccgcctg
      361 cttctaaaat ggccttgacg cgcgcggcaa tttcgccgtg aagattgtct acttctagat
      421 ctccaaatcc ggatttgcgc gcattctcga ccgcctctct atacacgata ttgtcttcga
      481 gagcctgggc gaaggtctgc gatgcaaaca gtaccaaaat caatgcgagc gtcttcatac
      541 tgggtaccat atcgaaccga aagggcctaa aatccagtgt gccggccaaa atacaaaatg
      601 aaagtaaaaa actatgatcg gtatcatagg aatccgggtc tcctgccctc atactacatc
      661 tagaggacaa aaatgcacag agccatcccc cccattcaga aatacatgac caccacgccg
      721 gtcacagttc agcagaatac aacgatcgca gaagcccaca aactcatgca ggaacacaac
      781 tttcgccatc tgcccgtgat gaagggaacc gaattctttg gcgttgtttc agaccgcgat
      841 ttgaaactga tggaatcttt tacaggagtg gatccaagca aagcgaccgt gggagacatt
      901 gctcacaaag acgcatacac catctccccc gacgcacaga ccgacgatgt ggtcaagacc
      961 atggcaacgc ataagtatgg ttcagctctc atcatcgata acaagaaagt agtcggaatc
     1021 ttcacaaccg tagacgccat gaacgccttc gcagaagtac tcgaagcact tctgaagaag
     1081 taaaaaatct gggcctctgc ggcgcatcaa tcacgcgccc gcagaggtct cgcaagaaac
     1141 caggcagaaa ccgcgaagac agaaagcacc actccgccca gaatcacggt aaacagttcc
     1201 cccttccatt cggcgattgc tcccgccaca atgcttccaa tcggcatggt tccgagaaaa
     1261 cacagagtgt acacactgat cacacggcct cgcaaatgat ccggcacaat ggtctgaaca
     1321 attgcgtttg ccagattgaa aacaaacatt acaaaaaagc caccggcgac aaggagcagg
     1381 ccagacaaaa taacgttacg cgacattgca aacccgagaa tcgccagtgg aaaaagcaga
     1441 agcccggtca gaagcatctt accccgtgat ccacgggcaa aagcagcaat cgcaaacgct
     1501 ccaagcaggg cacccaggcc ctgtgccccc tgcaaaagcc cgatctcgag agcagagccg
     1561 ccgagcatcg ttacagtcca cgctggaatg atcgtgatgt aacccagacc aaaaatagca
     1621 accatggcca cgatcagcaa gatgatgcga atgactggat gaccaaaagc aaatccgatt
     1681 ccttctgcaa tttcaattcg aagtgattct gctttccgtt tttcactggc gactgttcga
     1741 atcgcaagaa gagccacgat cactgccaga aaagaaaacg cattcacaag aaaacaaaat
     1801 cctgcaccga atgcaccata taccgcaccg gccgccgcag gtcccgcagc ccgcgcgaga
     1861 ttgaacatga tggcattgag cgcgattccg tttgttagat agtcgcgatc tacaagctct
     1921 gctacaaatg actgacgcgc gggtgcatca aatgcattgg caattccaag accaagggaa
     1981 agagcgatga tgtgccaggg ttctaccaga tgtgcgaaca cgagcgccgc gagaattgcg
     2041 gcgagaatca tcatagttgt ttgtgtgatg agaagcaaaa tccgcctcgg cattctatcg
     2101 gatacaactc cgccaaaaag ccccagcact gcaggcgcgc cggatgcaaa tccagcgtaa
     2161 cccagatacg caggagaatg agtgagttga taaatcagaa atgcctgcgc agtcgcctgc
     2221 atccacgtgc cgaccaggct acccagctgg cctgcaaacc agagtctgta gttacgaaat
     2281 ccgagggctg caaagacccc gggtggtttc acactttgac gccgccggag atttcgagta
     2341 tgacgccgtt tactagatcg ttctgtgcga tgaacaaagc ggaagccgca atttcttccg
     2401 gttttccgag ccttccgatt ggaatgacag attcccattt cgcaagtgcg tcgggtttca
     2461 tatctttcat gaccatatcc gttgcaatga atccaggcgc aattccagca gaacgaatgc
     2521 gaaatcgact caattcctgg gcccagagtt tagtcatcgc ggccacacct gccttggctg
     2581 cagaatagtt tgtctgccct ggatttccat gcatggaaac agaggcgatg gggatgatca
     2641 ctccctttga ttttgtctca atcatctgca ctgccgcttc tcttcctgtc aaaaatacgc
     2701 ccgttaaatt tacgtcgatg actgcctgcc attgatcgag actcagtttg cctttgacct
     2761 tgcctgtctc cttatccaca cgaaccagaa ggccatcgcg caagattcca gcattgagta
     2821 ctgccacatc cagtctgcct gtgcgcgaaa ctgcttcttt catcagcctt tctgcgtccg
     2881 cttctttcga agtgtccgcc gtcacggcga ttgctttgcc cccgcgcgct tccacctcat
     2941 tcacagcagc cgcgagacta tcttcgcgca aatcagagat cacgactaca gctcctgcgt
     3001 ctgcaaaagc aaaagacatt gccttgccca gtccgcctgc tgctcccgtg attacaactg
     3061 ttgcgccttt tatttccata agaacgacgt aagttcatcc tctctggcga tcattctttt
     3121 ttcttccgcc ctggatcttt catgatcgta tccaagaaga tgcagtactc cgtgcgaaag
     3181 aaggcgatag aattcgtcct ggatgctgtg gccaatttca cgcgcctgtc tgcgggccgt
     3241 gggaatagaa atcatgatat cgcccagagg aagtggcccc gcctgcggcc agtccgatac
     3301 caggcgcggc acacgagtgc gagtaaattc ctgggccgga aagctaagca catcggttgg
     3361 tttgtttttc ccgcgaaact ccgcgttcac gcgtctgatc gcaggatcag atactaaaga
     3421 aatctcaatg attgcgtttt tgtagcccag gtgtgtgagc cattgagtta gaactgtgcg
     3481 aattttgcgg tcagatggaa tcctgtcacg tacgtctatg cgaacccgac gatagattcg
     3541 atgcatcagc ccttgcgcgc ctgttccagt ttctttgtgg cttccatggc cggatactta
     3601 ggcctgtgat gaagtgaact gagcaaaatc tcagtgaatg ctttgcgaat cactgcaaga
     3661 tctcccatcg tgagccctga ttcatcgaat tgattttctg cgagcttaat attgatgatc
     3721 ttctggatca aagaatctac cgcggtctct gtaatttcgt ccagggagcg cgaagctgct
     3781 tccacagaat ctgcaatcat cacaatggca gtttctttgc tctgcgggat ggggccaggg
     3841 tagcggaagt tttctctctt tactttgcgt cttgatttct caagtgcctg atgatagaag
     3901 aaagacatgg tagaagtgcc atggtgttct ggaatgaatg ccatgacttc gcgcggaagc
     3961 ctgatctttc gcgcaatttc aattccatcg aggacatgat cgatgattac cttggccgct
     4021 ttttcaggat tgttcttatc aatgttttca ggctttggaa tgagatgctg attttctaca
     4081 aagaagccag ctcgcctggt ttttccaatg tcatggtagt aaacgcctgt tcgcgcgaga
     4141 agagtgttga gtccaagtcg ttcgcatgct ttttctgtaa ggcttgcaac agtcagagta
     4201 tgattccagg tagaaggtgc caggcgatag aactcctgta agagaggagt agaaggatca
     4261 gaaagctcct gcaagcggaa gcgagtgggc acattgaaaa cagattcgta cagaggcaaa
     4321 atcacaattg taaaggcagt ggccgcaccc atcaaaagtg ccttcaccat agcacttttg
     4381 tatccggtgg tcagcaaatc cccgccggac tgagaaatga tcactcgatt gagatacaga
     4441 taccccatgg ttacaagcac gccggcaagc agcatcaaga agatggaagc cgcaagaaag
     4501 tgaatgcgtt ttcgaatgcg tgtgccggcc catactgcca tcacagacat cacaagagaa
     4561 tgaaggaagg actgcggatc gtattgagaa acgccaaata cgagaagcga aaggtagact
     4621 gcagttgcca gagcaaagag ctctccggat agaagagaaa acaaaactgc aaatattcca
     4681 atgggaaccc acgatccaaa gatatgatct tccggattga tatttggctc atgccagagt
     4741 gtctcaatgg caaacagcat tccacagaag atccagatat tcatgaagaa tataatgttc
     4801 gctgacatgt cgctcatgcg tttggattca aatctgtacg cgaagaagac aagtatcatc
     4861 aaaagcaaaa agtgctgtag tgtaatggct gttatgcgct tcgctttctc gcgcaagtgc
     4921 aaccttgcat gaacttcgag agcggttcta ttttcctgtc ggatcacatc cccttgcttt
     4981 acaattggtt ctcccgctgc aattcgcatg tgcggaatat cca
//
LOCUS       DAOQJL010000265         5002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9873_length_5002_cov_3.783101, whole genome shotgun sequence.
ACCESSION   DAOQJL010000265 DAOQJL010000000
VERSION     DAOQJL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9873_length_5002_cov_3.783101"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PLG78_11760"
     CDS             <1..1168
                     /locus_tag="PLG78_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE23838.1"
                     /translation="VALGRSLFFDKELSGDRNISCSTCHSPAGFTGDALSTSIGAGGT
                     GENQLRVVSSGSMVARNAPPLFNLGAGNTRMFWDGRVSRDNATGALTTPESTLNGITP
                     ARADVASQLTSALAAQAMFPVASDVEMRGAVGNEIRAAADNQAVWAALMARLVGTNNG
                     TTGGMAAYRSLFSAAYPGVAYDDLNFGHAARAIAAFETASYAAVHSPLDEFLRGNPNA
                     LSADAKAGGLLFFGRAGCGGCHRGPLLSDANFHGIAAPQLGPGASGGDDQGRFLISGS
                     ANDNYHFRTPALRNVELTAPYTHAGAYTTLAAVIAHYSDTATGLRNYNGAGLRADYAA
                     TVDTNASHIQARLNARDPRVVRPLGLNATEQAQIEAFLKSLTDPSARNLSPDGN"
     gene            complement(1165..2742)
                     /locus_tag="PLG78_11765"
     CDS             complement(1165..2742)
                     /locus_tag="PLG78_11765"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNE23839.1"
                     /translation="MIPERPMDVKLVDVTDVPFDLSIATARTCYSSKGIITPEDVSRT
                     PESRALRDRIARSTLQAGHLTTRQHVHFVFAIDNASRHLVWSFLHSHPFYNSEQVSQR
                     YVEVKPDRFYVPLSLRQPGRERHASFYMDAVQYAVGIYQELLRELDVPAAAEFYKIFP
                     ARKSRAEVWQKTVHKKSMEAARYILPTAMHTYFYHTINGLTLHRYSRLCEACDVPEEA
                     RNLVESMVRAAREIDPLFAAEIPDSVPLESTPEYQFFKSYYGEESAPVSSQFIREFDE
                     SLGKRTSKLVSYDPGAVSVLASSVRAVLGATREMLSDESAVDLVLSPAKNSHLASALN
                     ETTLSRITRALQNVHYTFAKKISHTADSQDQRHRMVPGSRPVLMKHFTGKPDFVSPAL
                     ISSHSASREKYEEAMSVYFEKIERFVDAGGTEEETVYLLPNAFPVRFYESGDLLNLHH
                     KWKIRTCYNAQEEIFQASVDELQQAREVHPEIGKHIHAPCRIRKEAGVKPFCPEGDRY
                     CGVRVWEQDLSEYKRVI"
     gene            2759..3820
                     /locus_tag="PLG78_11770"
     CDS             2759..3820
                     /locus_tag="PLG78_11770"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNE23840.1"
                     /translation="MAGQSKVMSPESKPNASIRFRGIGSYIPSRRITNQHWESLVSTS
                     DEWIQKNIGIRERSRIGPEETTTDMGVHSAKLAIETAGVDPASIDFILCATNSQDDLW
                     PSTAAKIQERLGIKTTSLDLQAGCSGWLFGVRLGTSLLGSGQAKRVLVVGSDALTRFL
                     NYYDRDNMLFGDGSGSCILDLDPSYTARRLPVPIFDTGTLPSRAMALETIYTEKENAM
                     EKYLAREDMSLANRPIPSMEGRTAMKYALTKTKESIASVLSQARAMGIEKEDIALYAP
                     HQTNALVVQSLCEFAGFPIEKIPYTLDKYGGISTAGIPTGLYEHYRAGKLKKGDLVLS
                     SAYGAGFTYGAMIFEWGVD"
     gene            complement(3817..4347)
                     /locus_tag="PLG78_11775"
     CDS             complement(3817..4347)
                     /locus_tag="PLG78_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNE23841.1"
                     /translation="MDQYKEEKKRIRLVRADITKLSVDAIVNAANESLLGGGGVDGAI
                     HSAAGPDLLRACAALKGCKTGQAKITPGFQLPAKYVIHTVGPVYRGGMNKESELLASC
                     YQSSLAVASHNNVNTIAFPSISTGVYGYPIEEACRIAIVETLRYLSGHSEITEVTFVC
                     FSDRDLKIYERAMTEF"
     gene            4410..>5002
                     /locus_tag="PLG78_11780"
     CDS             4410..>5002
                     /locus_tag="PLG78_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNE23842.1"
                     /translation="MKSGESKLEPLKRLEKSIPGPLVFVIMDGVALAKGQAQGYPGNA
                     LDLSNAPCIKGLLASAPVQTTLQAHGTAVGMPSDEDMGNSEVGHNAMGAGRIFSQGAS
                     LVNQAIANKSLFAGKVWKKLIGNASEPGAALKGSAVHFIGLLSDGNVHSHIDQLISLL
                     QECSAQKVKEVYVHPLLDGRDVAKASAGIYIDKLTEAL"
BASE COUNT         1265 a         1314 c         1284 g         1139 t
ORIGIN      
        1 cgttgcgctc gggcgcagtc tcttctttga caaagaactg tccggagata gaaacatatc
       61 gtgctccaca tgtcacagcc cggctggttt tactggagac gcactctcta cttccattgg
      121 tgcaggcgga acaggcgaaa accaacttcg cgtcgtttca tctggatcca tggttgcgcg
      181 aaatgccccg cctctcttca atctgggggc tggcaatacg cgaatgttct gggacggcag
      241 agtgagccgt gacaacgcaa caggagctct gaccactccc gaatcaacgt taaacggcat
      301 cactccagct agagcagacg tcgcctctca gctcacatct gctctggctg ctcaggcaat
      361 gtttcctgta gcatcggacg tagaaatgcg aggagctgtt ggaaatgaaa tcagagctgc
      421 cgctgacaat caagcggtct gggcggccct catggcacgc cttgtgggaa caaacaacgg
      481 gacaacaggc ggaatggccg cctatcgttc cttgttcagt gcagcctacc ccggtgttgc
      541 gtatgatgat ctaaactttg gccatgcggc gagagccatc gctgcattcg aaacggcaag
      601 ctacgccgcc gtccactcgc ccctggatga attcctgaga ggaaatccaa atgctctcag
      661 tgcggatgcg aaagcaggag ggcttctatt ctttggccga gcaggatgcg gtggatgcca
      721 tcgcggaccg cttctgtctg acgcgaactt tcacggaatc gcagcacctc agcttggccc
      781 gggtgcttcc ggtggagatg atcagggtag attcttgatt tcgggatctg caaatgacaa
      841 ctaccacttt cgcacaccgg ctcttcgaaa cgtagagctc acggctcctt acactcatgc
      901 tggagcatac acgacccttg ccgcggtgat tgcgcactac agcgacactg cgactggtct
      961 gcgaaactac aatggagcgg gactgagagc agattatgcg gccacagtgg atacgaatgc
     1021 atcccacata caggctcgtc tgaatgcgcg agatccaaga gtggtacgtc cgcttggact
     1081 caacgcaacc gagcaggcgc agatagaggc attcttaaaa agtctgacag acccttctgc
     1141 gcgaaacctt tccccggatg gaaactagat gactcttttg tattcggaaa gatcctgctc
     1201 ccagactcga acaccgcagt agcggtctcc ttccgggcag aatggtttta ctccagcttc
     1261 ttttcgaatt ctgcacggcg cgtggatgtg cttgccgatt tctggatgca cttcgcgcgc
     1321 ctgctgcaat tcgtctacgg aagcctggaa gatctcttcc tgggcattgt agcacgtgcg
     1381 aatcttccat ttgtgatgca gattcaacaa atcgcctgac tcgtaaaagc gtactggaaa
     1441 cgcattgggg agaagataca cagtttcttc ttctgtgccg ccagcatcta cgaatcgctc
     1501 gatcttttcg aagtagacac tcattgcttc ctcgtatttc tcgcgagacg cagaatgaga
     1561 ggaaatcaga gcaggcgaaa caaagtctgg cttccctgtg aaatgcttca tgagaactgg
     1621 acgagaaccg ggcaccatac gatgacgctg gtcctgcgaa tctgcggtgt gggagatctt
     1681 ctttgcaaaa gtgtagtgca cattctgcaa ggctcgagtg atgcgagaaa gagtagtttc
     1741 attgagtgca ctggcgagat gcgaattctt cgcaggactc aatacaagat ccactgcaga
     1801 ttcatcagac agcatttccc tggttgcgcc gagcacggcg cgaacggaag aagctagaac
     1861 agaaacggca cctggatcat aggacaccag cttagaagtt ctctttccaa gtgattcatc
     1921 aaactcacga atgaactgag aagaaacagg cgcagattct tccccgtagt atgatttaaa
     1981 aaactgatat tccggagtgc tttccagcgg aacggagtcg gggatctcag cggcgaataa
     2041 cgggtcaatt tcgcgcgcag cgcggaccat ggactccacg agattgcgcg cttcttctgg
     2101 aacatcgcaa gcttcgcaca acctgctgta gcgatgcaga gtcaatccgt tgatggtatg
     2161 atagaagtac gtgtgcatcg cagtgggcag aatatagcgc gccgcttcca tggacttttt
     2221 gtgaacagtc ttctgccaga cttctgctct ggatttgcgc gccggaaaga tcttgtaaaa
     2281 ctcagccgca gccggaacat cgagttctcg aagtagttcc tggtaaatcc ccactgcgta
     2341 ctgaaccgca tccatataaa aggacgcatg cctttctcta ccgggctgtc tcagggaaag
     2401 tggcacgtaa aaacgatccg gcttcacttc cacgtaacgc tggctgacct gttctgaatt
     2461 gtaaaacgga tgcgaatgca ggaagctcca cacaaggtgc cgactggcgt tgtctatggc
     2521 aaagacaaag tgcacatgct ggcgcgttgt caggtgcccc gcctgcagag tggacctcgc
     2581 gatgcgatct ctgagtgcgc gagattcagg agttctcgaa acgtcttccg gggtgatgat
     2641 tcctttggaa gaatagcagg tccgtgcggt cgcgatcgac aaatcaaaag gtacgtcggt
     2701 tacatcgacg agtttgacat ccattggacg ctcgggaatc atagcctgaa gttccggcat
     2761 ggccggccaa tctaaagtta tgtcgcctga aagcaagcca aatgcaagca ttagattcag
     2821 aggaatcggc tcttacatcc cttctcgcag aatcaccaac cagcactggg aaagcctcgt
     2881 ttccacgtca gacgaatgga tccaaaagaa cataggaatc cgcgaaagat ctagaatcgg
     2941 gccggaagag actacaacgg acatgggagt gcattctgca aagctcgcca ttgagacggc
     3001 gggtgtagat cctgcgagca ttgacttcat tctatgcgcc acgaattctc aggacgatct
     3061 gtggccatcc accgcagcaa aaattcaaga aaggctcggg atcaaaacca catccctgga
     3121 ccttcaggca ggatgctctg gctggctctt tggagttcga ctaggaacga gtctgcttgg
     3181 ttccggccag gccaaaagag tgctcgtagt cgggtcagac gcgctcaccc ggtttctgaa
     3241 ctattacgat cgcgacaaca tgctttttgg agatggaagc ggctcttgca ttctggacct
     3301 ggacccatcg tatactgcaa gaagactgcc cgttcctatc tttgatacag gcaccctgcc
     3361 ttcgcgcgcg atggctcttg aaaccatcta cactgaaaaa gaaaacgcga tggagaagta
     3421 tcttgcgcgc gaagacatgt ctctggccaa tcgacccatt ccttctatgg aaggtcgaac
     3481 tgccatgaaa tacgcgctca cgaaaactaa agaatctata gcatctgtcc tatctcaggc
     3541 gcgcgctatg ggcattgaaa aagaagacat cgcactctac gcgccacacc agacgaatgc
     3601 acttgtggtg cagtctttgt gcgaatttgc tggatttcca atagaaaaga ttccttacac
     3661 tctggacaaa tacggtggga tctctacagc cggaattcca accggactct acgaacacta
     3721 tcgcgccgga aaattaaaaa aaggcgatct ggttctgtcc tctgcctacg gtgcaggctt
     3781 cacctatggt gccatgatat ttgaatgggg tgtggactaa aactctgtca tcgctcgttc
     3841 gtagatcttc aaatccctgt cggagaaaca aacaaaggta acttccgtaa tctcagagtg
     3901 gccggacaag tagcgcaaag tctccacaat ggcgattctg catgcctcct cgattggata
     3961 tccataaact ccggtgctga tagagggaaa tgcaatcgta ttcacgttgt tatgcgatgc
     4021 aaccgcaaga gaagattggt aacaggatgc aagcagctcg ctttctttgt tcatccctcc
     4081 cctgtacact ggtcctactg tgtggatcac atattttgcc ggaagctgga agccgggagt
     4141 gatctttgcc tggccggtct tgcatccttt gagtgcagca caggcccgca gcagatctgg
     4201 cccggcagca ctgtggatcg caccgtccac accacctcca cccagcaaag attcgttggc
     4261 tgcatttaca attgcatcca cagaaagctt tgtgatgtcc gctcggacca aacggattct
     4321 ttttttctct tctttatact ggtccatctg cactctcccg ccgggtcgtt tttccttgac
     4381 gcgtacttca ctgtctccag aaatcaagaa tgaaatccgg cgaaagcaaa cttgaaccac
     4441 tcaaacgcct cgaaaaatcc atcccgggtc cgctcgtatt tgtaatcatg gacggagtgg
     4501 ctctggcaaa aggccaggct caaggttacc ccggaaacgc actcgatttg agcaacgcac
     4561 cgtgcatcaa gggactactc gccagcgcgc ccgtgcaaac cactcttcag gctcacggaa
     4621 ctgcagtagg aatgccttcg gatgaagaca tgggaaacag cgaagtgggt cacaacgcca
     4681 tgggtgccgg tagaatcttt agccagggag caagccttgt aaatcaggca atcgcaaaca
     4741 agagcctgtt tgccggaaaa gtctggaaga aattgatcgg aaatgcaagc gaacccggag
     4801 ctgcgctcaa aggaagcgcg gtccatttca ttggcctttt gtcagacgga aacgtacaca
     4861 gtcatataga tcagttgatc agcttactac aagaatgctc cgcgcagaaa gttaaggaag
     4921 tttatgttca tcctcttcta gacggacgcg atgttgccaa agcgagtgcc ggaatctaca
     4981 tagacaaact cactgaagca ct
//
LOCUS       DAOQJL010000266         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9928_length_4984_cov_4.503956, whole genome shotgun sequence.
ACCESSION   DAOQJL010000266 DAOQJL010000000
VERSION     DAOQJL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_4984_cov_4.503956"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLG78_11785"
     CDS             complement(<1..443)
                     /locus_tag="PLG78_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNE23843.1"
                     /translation="MSTVLFPGLPLPLHIFEPRYQLMIQRVISRKEPFIVALIKSGQE
                     ALGDVAEPYEIGTTASIKQVENLENGRMNLLAIGVDRVKIHSTSRALPYLEGEMEVME
                     YPNEDLSQCAGSLRTVVQGFLSLVAQSRKIPRIELPEDPRELIFL"
     gene            complement(440..1336)
                     /locus_tag="PLG78_11790"
     CDS             complement(440..1336)
                     /locus_tag="PLG78_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNE23844.1"
                     /translation="MNGIATYLRMIKFSHTIFALPFAGIAFIQALPASQLISNGVPGF
                     AFYRMLVLVIVCMVSLRSAAMGFNRLADRNIDAVNPRTRMREIPSGALSVSAVRSFVI
                     ISLVLFVVAAFAISTTCGLLSPIAAGLVLGYSYTKRFTWLCHFVLGTAIGQAPAATAL
                     AMTGSIPLSALAWSGGLALYIGGFDILYACQDAEFDRSQKLHSIPSRFGIPTALWIAR
                     ISHILALCLFAFAAISSQTGLVFFAFLFCTGILFLVEHALVRPNRLEKIPIAFFNVNA
                     SISTVLFAGLLVDLWIRIPFFH"
     gene            complement(1333..2841)
                     /locus_tag="PLG78_11795"
     CDS             complement(1333..2841)
                     /locus_tag="PLG78_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HNE23845.1"
                     /translation="MKGKTLGFLRLTGILLRSAPFALDVVMEKSRQNPRPGFIQACLR
                     SMDGLFRALFPDASAARPHRLLRLLIQLGPTFIKIGQAMANRPDLVPLSYMHELESLQ
                     DRVPPFATWRAKRILRKELKRKGKTLSVFTVFPKTSIAAGSLGQVYECFLDDGTRVAV
                     KIQRPGIDKKIARDIEALRILARYAASHTGIGMGVNWDDVVEEFADTIRMELDFGREM
                     ENAEKFRDNFKAWKDIYVPIFYPEYCTKKVLVMEFIDGLKLNDLPALSAADKDPMDGV
                     RIVTRSYLKQLIQDGFFHADPHPGNLRLLEDGRVAFFDFGMAGSIDDEQKSMVLDLLI
                     HVAEQDIHAIVQDLANLGFLRTGHDFSAFQPAIEEVFRHYLGETERDRIQIHEIVYAM
                     SEIVYRYPFTIPTRFTYILRALLTLEGIGLSITPAFSFFRFARAYALDYFFLSQMGIL
                     AKKLLRGLIGSADLKDGWDKTKKFSRMLWNYVTNKNVLNLHLEGPEDPSEKQ"
     gene            complement(2890..>4984)
                     /gene="pbpC"
                     /locus_tag="PLG78_11800"
     CDS             complement(2890..>4984)
                     /gene="pbpC"
                     /locus_tag="PLG78_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNE23846.1"
                     /translation="DMSPAVSEAFLLREDKYFYFHPGVNPAALLKATRGYFSGSSARG
                     ASTITMQLARMKYGLETRSIRGKIFQILCAFYLEFRYSKSSILEAYLNLIPLGHNIEG
                     VPAASLIYFGKASSSLNPVEAITLCIIPPSPAKRTRNSSNTAMEIDQFRKQLFEQWIQ
                     KHPEDKELILSVGHATLPRDPLPFLAPHFSQMVLADPSNTDRSEIKTTLRASLQQTLE
                     RTISNYVQSRKSTGIKNAAALLVDVHTGKTLAHVGSANFADESIDGQVDGTRALRSPG
                     SALKPLLYALAVDRGIIHPRTLLKDTPIQFSTYSPENFEGEFLGPLSAQSALVLSRNV
                     PAIYIAAKLRQPDFTDFLKSSGVSLSKPKDYYGLAPILGGIEISMAQLASLYGALAKG
                     GVQRPVSYDGRTNAGKTIFSPEAAFITLEMLKSAREDGMAEEWMQSPFTIYWKTGTSS
                     GFRDAWTAGIFGSYVLVVWIGNFDGSSNPAFVGRDAALPLFLRIARAMAAENNSIKDW
                     IEAPAGVSRIEVCSVSGKIPRNFCPLRAHTWYIPGKSPIEPCDIHREVAVDLDTGLRA
                     CAGAKNTKKEVYEFWPSDIERVLKSAGLPHKAPPRFSPECQTADNHAEPPSIHSPVHG
                     VTYESSREGEEIALEAAADASVTDLFWFVDDGFVGKSKPGESLFWRAHPGEFKIRVVD
                     SGGASDQMTVKINAR"
BASE COUNT         1205 a         1202 c         1338 g         1239 t
ORIGIN      
        1 cagaggaaaa tcaattcacg aggatcctcc ggcagctcta tgcgcgggat ctttctggac
       61 tgggccacga gagacaggaa tccctgaact acggtgcgaa gagaacccgc gcactgagaa
      121 agatcttcgt taggatactc catgacttcc atttctcctt ccaggtaggg caaagcgcgc
      181 gaagtagaat gaatcttcac tcgatccaca cctatggcca gaagattcat tctgccattc
      241 tccaggtttt ccacctgctt gatggaagca gtggttccaa tttcgtacgg ttctgcaacg
      301 tcgcctaaag cttcctgtcc gcttttgatg agtgcaacaa tgaatggttc cttgcgagaa
      361 atcactctct gaatcatcag ctggtagcgc ggttcaaaaa tgtgaagtgg aagtggcaac
      421 cctgggaaga ggaccgtact caatggaaaa acgggatgcg aatccataga tctaccagca
      481 aacccgcgaa gagcaccgtg ctgatggaag cgttgacatt gaaaaatgca attgggatct
      541 tctccagcct gtttgggcgc acgagtgcgt gctctactaa gaataggatg ccagtgcaaa
      601 agaggaaagc aaaaaagaca aggcccgttt gtgaagaaat cgccgcaaat gcaaaaagac
      661 agagagcaag tatgtgtgag atgcgagcaa tccagagtgc ggttggaatt ccaaatctag
      721 atggaatgga atgcagtttt tgtgatctgt caaactctgc atcctggcag gcgtagagaa
      781 tgtcaaaccc tccaatgtaa agagcaaggc cgccgctcca tgcaagagca gacagcggaa
      841 tgcttcctgt catggcaagt gcagtggcgg ccggcgcctg gcctatggcc gtgcccagta
      901 caaagtgaca gagccaggtg aagcgctttg tgtaggagta gcccagtacc aggcctgcgg
      961 cgatggggga cagaagtccg caggtagttg agatggcaaa agcagccacc acgaacaata
     1021 ctaaagaaat tataacaaag gatcgaaccg cggacacact gagtgctccg gatggaatct
     1081 cgcgcatcct ggtcctggga ttcaccgcgt caatgttgcg atctgcaagg cggttaaatc
     1141 ccatggccgc gctgcgcagg ctcaccatgc agacaatcac cagcacgagc atcctgtaaa
     1201 aagcaaaccc cggcacgccg tttgagatca actgtgaggc gggcagtgcc tgaatgaatg
     1261 caatgcccgc gaaaggaagc gcgaagatcg tatgcgagaa cttgatcatt cttagatatg
     1321 tggcgattcc gttcattgtt tctccgaggg atcttctggg ccttcgaggt gaaggttcag
     1381 tacattctta ttggtcacat agttccagag cattcgcgaa aatttctttg ttttgtccca
     1441 gccatccttg aggtccgctg atccgatcag accacgcaac agctttttgg cgagaattcc
     1501 catttgagat aagaagaaat aatccagtgc ataagctcgt gcgaagcgaa agaaactgaa
     1561 agcaggggta atggacagtc cgattccttc cagggtaagg agcgctcgca ggatgtaggt
     1621 gaatcgcgtt ggaatggtga aaggataacg atagacgatt tctgacatgg cgtaaacgat
     1681 ttcgtgaatc tgaattctgt cgcgctctgt ttcacccagg tagtgacgga atacttcttc
     1741 aatcgcgggt tgaaaagcgc tgaagtcgtg gccagtgcga agaaagccca gattggccag
     1801 gtcttgcacg attgcatgga tgtcctgttc tgccacatga atgagcagat ccagcaccat
     1861 cgacttctgt tcgtcgtcta tgcttcctgc cattccaaaa tcgaagaatg ccactctccc
     1921 atcttcgagg aggcgtaaat tcccaggatg cgggtccgca tgaaagaagc catcctggat
     1981 caactgtttg aggtacgacc tcgtgacgat gcgcacaccg tccatgggat ctttgtctgc
     2041 tgcactcaat gcaggaagat cgttgagttt aagtccgtct atgaactcca tcacgagcac
     2101 tttctttgta cagtactccg ggtagaaaat tggaacgtaa atgtctttcc aggctttgaa
     2161 gttatcgcgg aatttttctg cgttttccat ttcgcgaccg aagtccagtt ccattctgat
     2221 tgtgtccgcg aattcttcta caacgtcgtc ccagtttact cccataccga tcccggtgtg
     2281 agatgccgca taacgtgcaa gtattctcag tgcttcaatg tcccgcgcga tttttttatc
     2341 tattcccggt ctctggattt tcactgcaac ccgggttcca tcgtctagaa agcattcgta
     2401 gacctggccc aggcttccgg ctgcgatcga tgtttttgga aaaactgtga aaacgctcag
     2461 tgtctttcct tttcgtttca gttcttttct tagaatgcgc tttgcgcgcc aggttgcgaa
     2521 aggcggcacg cggtcctgga gactttccag ttcgtgcata tatgaaagag gaaccaggtc
     2581 cgggcggttg gccattgcct ggcctatctt gatgaaagtt ggtcccagct gaatgagaag
     2641 gcgcaaaagg cgatgaggtc tggcggcgct cgcatcaggg aaaagcgcgc gaaaaagccc
     2701 gtccatgctt cgcaggcatg cctgaatgaa tccgggccgg gggttttgcc gtgatttttc
     2761 catcacgacg tcgagcgcga aaggagcgct tctcagaagt attccagtga gccgcaggaa
     2821 cccgagggtc ttgcccttca ttctggtagc tcggagcggg gaataaggag tgcaatcgat
     2881 ttttgcggat catcgcgcgt tgattttgac tgtcatttga tcgctggcgc ctcctgagtc
     2941 cacaactcgt atcttgaatt cccctggatg agcgcgccag aagagagact ctcccggctt
     3001 tgattttcca acaaagccat catctacaaa ccagaataga tctgtgacgg atgcatccgc
     3061 ggctgcttca agagcgatct cttcgccttc gcgtgaagat tcatacgtca ctccatgcac
     3121 gggcgaatgg atggagggtg gctctgcgtg gttgtccgca gtctggcatt cgggggaaaa
     3181 tcgcggaggc gctttgtgcg gcaggccagc actcttgaga actctttcta tgtcagacgg
     3241 ccagaactca taaacttctt tctttgtatt ttttgctccg gcgcatgctc tcagtccggt
     3301 gtccagatct actgcgactt cgcggtgtat gtcgcaaggt tcgatcggag actttccagg
     3361 aatgtaccat gtatgcgcgc gaagcgggca gaagttcctc ggaatttttc cggaaaccga
     3421 acacacttca attctgctca ctccggcggg agcttcgatc caatctttga tgctgttatt
     3481 ttctgcggcc attgcgcgcg cgattcgcag aaacaaagga agggccgcgt ctcgccctac
     3541 gaaggcggga ttggaggaac cgtcaaagtt cccgatccac acaaccagga cataggaccc
     3601 gaaaattccc gcagtccagg cgtcacgaaa gccggatgac gttcctgttt tccagtagat
     3661 ggtgaatggc gattgcatcc attcttctgc cattccatct tcgcgtgcgg acttgagcat
     3721 ctccagtgta atgaatgcag cttctgggct gaagatggtt tttcctgcat tcgttctccc
     3781 atcgtaagag actggtctct gcactcctcc tttggcaaga gctccgtaga gtgacgcaag
     3841 ctgtgccatt gagatttcga ttccgcccag aatgggagcg aggccatagt aatcctttgg
     3901 tttggataga gatactccag atgatttcaa gaagtctgtg aagtctggtt gtctgagttt
     3961 tgctgcgatg tagatggcag gcacattcct gctcagaacc agcgccgatt gagcagataa
     4021 cgggcccaaa aactctcctt caaaattttc cggactgtag gtcgagaact ggataggcgt
     4081 gtctttcaac agtgttctcg gatgaatgat tccacggtct acggccagag catagagaag
     4141 aggcttcagt gcggatccag gagatctcag tgcgcgagtt ccatccacct gtccatcaat
     4201 gctttcgtct gcgaagttgg cgcttccaac atgagcgagt gtctttcctg tatgcacatc
     4261 cacgagcaaa gcggcggcat tcttgattcc agtcgatttt cgcgactgga cgtagttcga
     4321 aatcgttctt tccagagttt gttgcagact tgcgcgcaga gtggttttga tttctgatcg
     4381 gtctgtattc gaggggtctg ccaggaccat ttgcgaaaaa tgcggtgcga gaaaaggaag
     4441 aggatctcgc ggaagcgttg cgtgacccac agagaggatg agctctttgt cttcagggtg
     4501 tttctggatc cattgttcaa agagttgttt gcgaaactga tctatctcca tggcagtgtt
     4561 gcttgagttg cgggttcgtt ttgccgggct tggaggaatg atgcaaagag tgattgcctc
     4621 cacaggattg agagaagagg acgcttttcc aaagtagatc aaggaagcag ccgggactcc
     4681 ttctatattg tggccgagtg gaatcaggtt caggtatgct tccaggatag acgattttga
     4741 ataacgaaat tctagataga atgcgcatag aatttgaaag atcttgccgc gaatcgatct
     4801 ggtttcgaga ccatacttca ttcgagccag ttgcatggta atcgtggaag caccgcgcgc
     4861 agaactgccg ctgaaataac ctcttgttgc tttgagcaga gcggcagggt tcactcccgg
     4921 gtgaaagtaa aagtatttgt cttcgcgcaa caggaatgcc tcggatacgg caggcgacat
     4981 atct
//
LOCUS       DAOQJL010000267         4977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_9953_length_4977_cov_5.217798, whole genome shotgun sequence.
ACCESSION   DAOQJL010000267 DAOQJL010000000
VERSION     DAOQJL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9953_length_4977_cov_5.217798"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLG78_11805"
     CDS             <1..1033
                     /locus_tag="PLG78_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23847.1"
                     /translation="KNPTLKNTILAFHGLAKKRVPTWQEIVDEMGVGKPVLKRYRAPE
                     SVMQQIDGMIKKHHTQIAMRKAEMREIENLKRNFFDYDGAGKVKTDFLNPIVAEVLRR
                     IYNEKMVNDMTAKSHKTEPPKLLFVVMKDFDISSLYLLESSVHARDGGNPIEIILFKQ
                     GLFKATIDEFNALLRDIEVFFKKNKNMSFHFHNLTEVLKKDSGDEEMRRFVTLCRRAT
                     KLFRIVGTSFRTIWDNHIAAKSDTNQATQEKLARSKTIPIESLAVGKRYIPFYDLEIV
                     SNNRWNGMTVEGVIEQIVRNMYNYMLIFRDDELLGHLSSPARLQAEIQSLIDELKKLG
                     VEYDDGASD"
     gene            1075..2286
                     /locus_tag="PLG78_11810"
     CDS             1075..2286
                     /locus_tag="PLG78_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNE23848.1"
                     /translation="MAIQKIGILTSGGDCGGLNGVIIGAARLALSRGIKAYLIPNGYA
                     GLYNLVDFPGLVELTESRIEDIHVYLAGSEAGNSRVKISKIKDEKKYDRIREGLKKFG
                     IDALVISGGDDSGSVVVDLASQGIPCVHAPKTMDLDLMPYSVGGDSTVARIAEFTRDL
                     YTTGRTHNRIVVMEVFGRYAGHTAFRGGIAAGADAILIPEVPVDFDLLYKHVMGNFIR
                     RVESSDARKGTAAVIVAEGLKDSSGKELVDESSTADAFGHKKLMGAGKYVVKQLEDRV
                     KKDPAVKEFMKRTGQFVEGVYEIPEIREVRPGHLVRCGFTSPVDANFGLEVGAAAVEL
                     HLKGINGVTVISYQDGKIRYMPVAEAIVQRHVDIESIALYERMGICFGRAPANVEPKF
                     EKFSGRPERVY"
     gene            complement(2346..3083)
                     /locus_tag="PLG78_11815"
     CDS             complement(2346..3083)
                     /locus_tag="PLG78_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE23849.1"
                     /translation="MQESSEVSMKIPFFERRRRIRRPWTLLFFGIAFLLLPFLNYLGI
                     TSQLQISPQMPVVALKLLNPVELILLFTPILVGIGLLAVKRWGWWLFLAYAVVLIVHN
                     IYALARSPGYYNAGALIQTILGVAAVLYFVRRDISAPYMRMYPRGWRIQKRKPVEVEV
                     LVDGIRRSTRDVSDSGLYVAWEDCYREPGEEVHLAFFLDGAEYKCRAGVVRVDEDGAG
                     IAYRGVTRAFKKNIMRDIAKLENLNQP"
     gene            3184..>4977
                     /locus_tag="PLG78_11820"
     CDS             3184..>4977
                     /locus_tag="PLG78_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE23850.1"
                     /translation="MRLLLALLLLCGCHGQIVQAPPSVPISETLSDAYDVGYERERKI
                     RSTDWMSGVNPDVVRTLVGTSEGSAATLEKVLARGGNHPYIAWVMVQRLAGFRMAHGD
                     YAGAIAIWQKYSNYFPFRQMEVAQLIGLLARGDRANVQSVPGINSTANDYLPIPELSG
                     RRMYFTAFDRPGGKGKEDIWKTEQGDSGWTTPAIVKELCTSKSESPDGLSPDGTILSV
                     FGNYDGAYGNGDIFESRLGADGWSSLRHLPAPINSNYFESDSFYTADGNALIFSSDRP
                     GGYFTYRPVTELKGGFLMGNTDLYVAFRKPDGTWSTPRNLGPYLNTPGAERTPFLYAD
                     GKTLYFSSDGYNGFGRSDVYKSVRLDDTWLHWSTPENLGPLVNGAGQDWGFRLTAASD
                     RGYFSGELPGTVGGQDIFEITPLPPAIRPASIVMAVRGTVRDDMSVPLESQIEWQDLA
                     TGQSLGIMSSKPVSGEFFIALPAGREYAYFARKPGYFPVSQRVDLRNTQSYFEKTVDI
                     KLVSIASAQAKNVEIVLNNIFFESGSATLNPRSFSELDRLAVILKQNPNLRVEIQGHT
                     DDVGQPAANLELSANRARSVLQYLVQAGISRQ"
BASE COUNT         1367 a         1220 c         1267 g         1123 t
ORIGIN      
        1 gaaaaaccct acgcttaaga acactattct agcgttccac gggttggcaa agaaacgcgt
       61 tcccacctgg caggaaatcg tagacgaaat gggtgtgggc aaacccgttc tgaaaagata
      121 ccgcgctcct gaatccgtca tgcagcagat agacggaatg atcaaaaaac atcatacgca
      181 aatcgccatg cgcaaggcag aaatgcgtga aatcgaaaat ctcaaacgta atttctttga
      241 ctatgacggc gctggaaaag ttaagactga ttttctcaat ccgatcgtag cggaagtcct
      301 caggcgcatt tacaacgaaa aaatggtcaa cgatatgacc gcgaaatcgc acaaaaccga
      361 gccgccaaag ctattgtttg tggtgatgaa agactttgat atctcgagcc tctaccttct
      421 agaaagctct gtgcacgcgc gcgacggcgg aaatcccatt gaaatcattc tgttcaaaca
      481 gggcttgttc aaagcaacca tcgatgaatt caatgcattg ctgagagaca tcgaagtgtt
      541 tttcaagaag aacaagaaca tgagctttca ttttcacaat ctcactgaag tgctcaagaa
      601 agacagcggg gatgaggaaa tgcgacgctt tgttactctc tgccggcgtg cgaccaaact
      661 ctttcgcata gttggaactt cgtttcgcac gatctgggac aatcacatcg ctgccaaatc
      721 tgatacgaat caggcaaccc aggagaaact tgcaagatcc aaaacaattc ccattgagtc
      781 tctggcagtc ggaaagcgct atataccatt ctacgacctg gaaatcgtat ccaacaaccg
      841 ctggaacggc atgacagtgg aaggtgtgat tgagcaaatc gtcaggaaca tgtacaacta
      901 catgctgatc tttcgcgatg atgaactact cggacacctt tcgtctcctg ctcgactgca
      961 ggctgagatc caatctctca tagatgagct gaaaaaactg ggtgttgagt atgacgatgg
     1021 cgcgtccgat tgaatttgcg ttgactcatt tttagcctga agatgattca gggcatggca
     1081 attcagaaaa tcggcatcct tacttccggc ggagactgtg gaggtctcaa cggagtcatc
     1141 attggcgcgg cgcgcctcgc actatctcgc ggcatcaaag catatttgat cccgaatgga
     1201 tatgccggcc tttacaatct ggtagatttt ccgggtctgg tcgaactcac ggaatcgcgc
     1261 atcgaagaca ttcacgtata cctggctgga tcggaagcag gcaactcacg cgtgaagata
     1321 tccaaaatca aagatgaaaa aaagtacgac agaatccgcg aaggcctgaa gaaattcgga
     1381 atagatgcac tcgtcatatc gggtggagac gattccggaa gcgtagttgt ggatcttgct
     1441 tcgcagggaa ttccatgcgt acacgctccc aaaaccatgg acctggactt gatgccttac
     1501 agtgtaggtg gagacagcac ggtagcacgc atcgcggaat tcacgcgcga tctatacaca
     1561 actggccgca ctcacaatcg aattgtagtc atggaagttt ttggccgtta tgcgggacac
     1621 actgcatttc gcggtggaat cgcagccggt gcggacgcaa ttttgatacc ggaagttcca
     1681 gtcgattttg accttcttta taagcatgtg atgggcaatt tcatcagacg agtggagtca
     1741 tcggatgcgc gcaagggcac ggctgcggtg attgttgcag aaggactcaa agattcttcc
     1801 ggcaaagaac tcgtagatga aagctcaaca gcggatgcgt tcggtcataa gaagctcatg
     1861 ggtgctggaa aatacgtggt gaaacaacta gaagatcgtg taaaaaaaga tccggctgtg
     1921 aaagaattca tgaaacgaac tggccaattt gtagaaggag tgtatgagat tccggaaatt
     1981 cgcgaagttc gccccggcca tctagtgcgc tgcggattca caagtcctgt ggacgcaaac
     2041 ttcggactcg aagtaggtgc ggctgcagtg gaactccatc tcaaaggaat caatggtgta
     2101 actgtgattt cctatcaaga cggaaagatt cgttacatgc ctgtcgcaga ggcgatcgtt
     2161 cagagacatg tagacattga gagcattgca ctctacgaga gaatgggcat ttgctttggt
     2221 cgggcaccgg cgaatgttga accaaaattt gaaaaattct cgggaagacc agaaagagtc
     2281 tactgatcca ggagacaacg ccgcctgcga aggcggcgtt tgcacgacca aaagagaatc
     2341 gcgcgctagg gttggttgag gttctccagc ttagcaatgt cgcgcatgat gtttttctta
     2401 aatgcacgag tgactccgcg ataggcaatg cctgcgccat cttcgtcgac tcgcaccaca
     2461 ccggctctgc atttatactc agcaccatcc agaaagaaag ccagatggac ttcttctcct
     2521 ggctcgcgat agcaatcctc ccatgctaca tacaatcctg aatcacttac gtcgcgtgta
     2581 gatcgccgaa tgccatccac cagcacttcc acttctaccg gtttgcgttt ttgaattctc
     2641 catccgcgcg gatacattcg catatacggt gcagaaatat cgcggcgcac gaagtacaaa
     2701 actgcggcca ctccaagaat cgtctggata agagctcctg cgttgtagta tcccggagaa
     2761 cgagccagag cgtagatgtt atgaacaatc aacacgaccg catacgcgag aaacaaccac
     2821 cagccccatc tcttcaccgc gagaagtccg atcccgacga gaatgggagt gaaaagcaga
     2881 atcagttcca caggattgag aagcttaagg gcgacgactg gcatctgtgg cgaaatttgt
     2941 agctggcttg tgattcccaa ataattgaga aacggtagca acaggaaagc aatgccaaag
     3001 aacaacagag tccatgggcg gcgaattcgt cttcttctct cgaaaaaagg aatcttcatc
     3061 gagacttcag acgattcttg catgcctttt tctaaggtcg ccggtcaaca gatgcaacca
     3121 ctttccgcgt gactgaggaa attattcctt gaacacgtca tgcgatgagg caattcatcc
     3181 tgcatgaggt tgcttctggc ccttctgctt ctctgcgggt gccacggcca gattgttcaa
     3241 gctcctccct ccgtgcccat ttcagaaacg cttagtgacg catatgatgt gggctatgag
     3301 agagaaagga agatcagatc cacggactgg atgagcggag taaacccgga cgttgtacgc
     3361 acactggttg gaacatcgga gggcagcgct gccactctgg aaaaggtcct ggcacggggc
     3421 ggaaaccacc cttacatcgc atgggtgatg gtgcagagac tggctggctt tcgcatggcg
     3481 catggagact atgcaggcgc catcgccatc tggcaaaaat attcgaacta ctttccgttt
     3541 cggcagatgg aagtggcaca actcatcgga ttgcttgcaa gaggagatcg cgcaaacgta
     3601 caaagcgtcc ccgggatcaa cagcaccgct aacgactatc ttcccattcc ggaactgagc
     3661 ggccgcagaa tgtacttcac tgcattcgat cgacccggtg gcaaaggaaa agaagacatc
     3721 tggaaaacgg aacaaggcga ctcaggatgg acaacacctg cgatcgtcaa agagctctgt
     3781 accagcaaat cagagtcgcc tgacggtcta tctccagacg gaacaattct ctctgtattt
     3841 ggtaattatg acggcgcgta cggaaatgga gacatctttg aatcccggct cggggcagac
     3901 ggttggtctt cgctacgcca tctaccggcg cccatcaact caaactactt tgaatctgat
     3961 tcgttttata ctgcagatgg aaacgcattg attttttcaa gcgatcgccc gggtggatat
     4021 tttacataca gaccggtgac tgagttgaaa ggcggattct tgatggggaa tacggatctc
     4081 tatgtcgcct tccgcaaacc agacggaacc tggagcacac cgcgaaatct gggaccttac
     4141 ttaaatacgc cgggagcgga acgtacgcca ttcctatacg cagatggaaa gacgctctat
     4201 ttttcgtcag acggatacaa cggcttcgga agatcagatg tatacaaatc tgtaagattg
     4261 gatgatacgt ggcttcattg gtctacgcca gaaaatctag gacctctcgt aaatggcgca
     4321 gggcaggact ggggtttcag actcacggct gcatcagacc gcggttattt ttctggagag
     4381 ttgccaggaa ccgtgggagg ccaggacatt tttgaaatca ctcctcttcc tccggcgatt
     4441 cgcccggcgt ccattgtgat ggcagttcga ggcacggtgc gcgacgacat gagcgttccg
     4501 ctggagagcc agattgaatg gcaggacctc gccaccggcc agagccttgg aatcatgtcg
     4561 agcaaacctg tgagcggaga attctttatc gcactgcccg caggaagaga gtacgcctac
     4621 ttcgcacgca aacctggata ttttccggta agccagcgcg tggatctgcg aaatacgcaa
     4681 agctacttcg aaaagactgt ggatataaaa ctcgtttcga ttgcgtctgc tcaggctaag
     4741 aatgttgaaa tcgtgctgaa caacattttc ttcgaatccg gaagtgccac actcaacccg
     4801 agaagctttt cggaactgga tcgactggcc gtcatcctaa aacagaatcc taatcttcgc
     4861 gtagaaatcc agggacatac agacgacgtt ggacaacccg cagccaatct tgaactgagt
     4921 gcaaacagag ccagatccgt cctccaatac ctggttcagg caggcatttc gaggcaa
//
LOCUS       DAOQJL010000268         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10065_length_4942_cov_4.007571, whole genome shotgun sequence.
ACCESSION   DAOQJL010000268 DAOQJL010000000
VERSION     DAOQJL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10065_length_4942_cov_4.007571"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..488)
                     /locus_tag="PLG78_11825"
     CDS             complement(198..488)
                     /locus_tag="PLG78_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23851.1"
                     /translation="MKILFTAILALAALTALPTSVFADKCVLCGSGSTNGCQQCRLSN
                     GVDNQANRKACESRGCKITGTGSCSTAANVKVCHAPSGSQQDVTFAWPAPAR"
     gene            656..1294
                     /locus_tag="PLG78_11830"
     CDS             656..1294
                     /locus_tag="PLG78_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HNE23852.1"
                     /translation="MRKFLLFFLALAGCIPEPRRIPGDPLDSPLLDYLLFRSTRTASC
                     ADRGFCYIYSSQNPPATGSYGGIAGADAMCSQDAARPAAATYPIKAFLVGTTRRASLT
                     ASAGDGQLDWVLRSNQQYRRSDGTTVIGTTGSNALFTFPLAASFGTVGIGFRTGLNVN
                     WTTVGNDCLSWTDGSVGQTSEIGTANQTNNTAIGGGNITCSNPGYYLMCVEQ"
     gene            1383..2024
                     /locus_tag="PLG78_11835"
     CDS             1383..2024
                     /locus_tag="PLG78_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23853.1"
                     /translation="MFNKATTAVLSAALLLAGCGPSIITNSKAKFPVPVADNAPAFLF
                     PINLSHAGISGDSKTQGISVSAGIAAKFGKKVVSGQQLFDQVGNLSYELAEAISSQAK
                     AGTWTMTGPAATVASSLSTTMTGILSKLVSLNLIPAGYKFKYIIAVHSHGSAGMAPKT
                     VVVNSWGGIYDIESKEILDYIESSDTMVDDEKVALGNFPIVYNGIIQHLLDGK"
     gene            2080..2472
                     /locus_tag="PLG78_11840"
     CDS             2080..2472
                     /locus_tag="PLG78_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23854.1"
                     /translation="MIRILLTSLLVFSSFACGKKEPVADSELIGTWRGSVLIVDVEFI
                     FTKDSYQQRTFSFNNVMIGGTKGSMTVEGPVIRVHQTQEYRYDNDTRKGAWFDKEANY
                     TISYKISGNNISLHPSTSSETMELSKEQ"
     gene            2469..3173
                     /locus_tag="PLG78_11845"
     CDS             2469..3173
                     /locus_tag="PLG78_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNE23855.1"
                     /translation="MKSVAPRIFACVLLFAVVSNCMTVLTLTNYKDRYQPVLYSGVRT
                     SLGYDPHAHNPLAGLVQTLMFIDLPFSAVLDTLILPATLPLAAFAQSKDLAFERQGNP
                     YVRLVLPPVLDLLLLQGNYSLEDGQNPFPPASRDAQQSDNSTYKLIHLPVKNLEAGVP
                     GCYLHCATREEASGLFSLGRSGGVSWRTLGLIRVKGVYKRERPYRNETFQYCLPDGGT
                     RGDDNVQSMAINRMWQ"
     gene            complement(3282..4229)
                     /locus_tag="PLG78_11850"
     CDS             complement(3282..4229)
                     /locus_tag="PLG78_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23856.1"
                     /translation="MTSAQEAQEALAAGEFARAKDIYGSLLEEYPEESDYICGFYTSA
                     YWDNRHSMILEAPPGKARGSALVEAWDQFEALANDKEFSGLSSFRTAMRSILGRAAEE
                     FRLSFQDSGLTHDSRTLAELGRCLIRIEDYANAAEILHFARKVRSTPEVLFLLGEALC
                     ASAAGDPETLAKGLSYYRDAFILEPDIIDPSLIASHPASDVFTRVYEEKDQNKELASV
                     WFPAKLMSLAQRLPLRRLLPEEVKEISKDLARLSPDLERVVEKYKERVRARICFFILV
                     LLQHHYLHDYNEEAIQELETRLAELDAHLYKEYRDYLHG"
     gene            complement(4230..4721)
                     /locus_tag="PLG78_11855"
     CDS             complement(4230..4721)
                     /locus_tag="PLG78_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNE23857.1"
                     /translation="MATEYSFDISSKVDRQELTNALDAVRKEVENRFDFKGSPVTIDL
                     EKDTLNLEAQDEMKLKQLIDVIQSKLVKRNISLKAFKFGPFESNVSGKFKCKTEIQSG
                     LNQEQCKKITKIIKESKLKVQSRIQEDQVRVTGKDRDELQAIIRMIKEANFDFDTSFS
                     NYR"
     gene            complement(4744..>4942)
                     /locus_tag="PLG78_11860"
     CDS             complement(4744..>4942)
                     /locus_tag="PLG78_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE23858.1"
                     /translation="LIFMANRGAVEAGVDCGKLIREASGLIGGGGGGKPEMAQAGGKD
                     ASGLESAVQRAKGLLKESLHS"
BASE COUNT         1106 a         1254 c         1256 g         1326 t
ORIGIN      
        1 gtgcggggcc tggaaagatt catctttatc gcggtaagga aatagttaag aaaagtatac
       61 catctgagat tgcagatgaa gagctggtgc ggctgatcca ggagcacgga atgtgggtgg
      121 agccaggggc aggttgagca ctggattatg gaaaggagag ggacgtttgc acgtccctcc
      181 aatggatcga ctggttgtta tcgcgcgggc gcaggccacg caaaggttac gtcttgctgg
      241 ctcccggaag gagcgtggca aacttttaca ttcgcagcag tggaacagga tccagtgcct
      301 gtgattttgc atcctctgga ttcgcatgct ttccgatttg cctggttgtc tacaccgtta
      361 gacaatctgc attgctggca tccgtttgtg cttccgcttc cgcagagcac gcatttgtct
      421 gcgaaaacgc tggttggcag tgctgtgagt gctgctagtg ccaggatcgc cgtgaacagt
      481 atcttcatgt tatcccccgc aagatccaaa tgattgaatc ttcgcattgt ttgccaattt
      541 ttgtggttcg cgtttgcagt cgtctactgg tttttgatat cccgcggagt taaaaaaaaa
      601 ctgtttcata acatgatctt gcgactcatt ccaagaaaag atcgtccaac cgcagatgag
      661 gaagtttctt ctatttttcc tggctcttgc cggatgcatc cctgaaccgc ggcgcattcc
      721 aggggatcca ctggattctc ctctgctaga ctatttgctg tttcgcagca ccagaaccgc
      781 aagttgtgcc gatcgtggtt tctgctatat ttactcatct cagaatccac ccgcgactgg
      841 tagctacgga ggcattgcag gtgcagacgc gatgtgctcg caggacgcgg cgcggccggc
      901 ggcagcaaca tatccgatca aagcatttct tgttggaacg actcgccgtg catctctcac
      961 tgccagtgca ggggacggac aactcgactg ggtcttgcgt tctaatcagc aatacagacg
     1021 atcagacggt acgactgtca ttggaactac aggatcgaat gctcttttta ctttccccct
     1081 ggctgcatcg tttggaacgg tagggattgg ctttagaact ggattgaacg tcaactggac
     1141 aaccgtgggc aatgattgtc tgtcatggac ggatgggtca gttgggcaga catcggagat
     1201 tggcactgcc aatcaaacca acaacactgc catcggaggt ggcaacatca catgttccaa
     1261 tccaggctac tacctgatgt gtgtggagca gtaaattctg tttatttcct tgccctgtaa
     1321 aagacccccg gcatacctcg gttctggccc cattcggggt cattgatctc ggaggatatt
     1381 caatgttcaa taaggcaact acggcagtcc tttccgcagc tcttctgctg gctggatgtg
     1441 gaccgtccat catcacgaat tcaaaagcga agttcccggt tccggtagcg gacaatgcac
     1501 ctgcattcct gttcccaatc aacctttcgc acgctggaat ctctggtgat tctaagactc
     1561 aaggaatcag cgtaagcgct ggcattgcag ctaaatttgg aaagaaagta gtatccggtc
     1621 agcaactgtt tgatcaggta ggaaacctct cttacgaact ggcagaagcg atttcttctc
     1681 aggcaaaagc tggaacatgg actatgactg gtccggctgc aactgtagct tcttctctgt
     1741 ccacaaccat gactggaatc ctcagcaagc tagttagctt gaacctgatt cctgctggtt
     1801 acaagttcaa gtacatcatc gcagttcact cacatggatc tgctggtatg gcacctaaaa
     1861 ctgtagtagt aaacagctgg ggcggtatct atgatatcga aagcaaagag attctcgatt
     1921 acatcgaaag ctctgacaca atggtagacg acgaaaaagt tgctctgggc aacttcccaa
     1981 tcgtatacaa cggtatcatt cagcaccttc tcgacggtaa gtgattcaat cactccgggg
     2041 ccgcctgcca ttctgcgggc ggtctatctt ttaaaaacca tgattcgcat tctactaact
     2101 tctcttttag ttttttcctc tttcgcctgc ggcaaaaagg aaccggtcgc cgattctgag
     2161 ctgatcggta catggcgtgg gtcggttctc atcgtagacg tggaattcat attcacgaaa
     2221 gactcctatc agcagagaac cttttccttt aacaacgtga tgattggcgg aaccaaggga
     2281 agtatgactg tggaaggccc tgtgattcgc gtgcaccaga cccaggagta tcgctatgac
     2341 aacgatacgc gcaagggcgc ctggtttgac aaagaggcaa attacactat ttcgtataag
     2401 atttccggga acaatatctc ccttcatccg tccacttcct cagaaaccat ggagctttcc
     2461 aaagaacaat gaagagtgtt gcgccgcgca ttttcgcgtg cgtcctcctg tttgcagtgg
     2521 tttcgaattg catgacggtt ctcactttga ccaactacaa ggatcgctat cagccggttc
     2581 tgtattcggg ggtaagaaca agtttggggt atgatcctca tgcgcacaat cccctggccg
     2641 gtctggttca gactttgatg ttcattgatt tgcctttcag tgctgtcctg gacactctga
     2701 ttcttcctgc gaccttaccg ttagccgcct ttgctcaaag caaggatctt gcattcgaac
     2761 ggcaggggaa tccttatgtt cgtctggttc tgcctccagt gctggattta ctcttgcttc
     2821 agggtaacta ttcgctcgag gatggacaga acccctttcc tccagcttcg cgggacgcgc
     2881 agcagtcgga caattccacg tacaaattga ttcaccttcc cgtgaagaat ctggaagccg
     2941 gcgttccagg ctgctatttg cattgcgcca cgcgcgagga ggcctctggc cttttttctc
     3001 tagggcgcag cggaggcgtt tcgtggagga ctctcggctt gattcgagtc aaaggcgtct
     3061 acaagagaga gcgaccctat cgcaatgaaa cctttcagta ctgtttgccg gacggcggaa
     3121 cgcgagggga tgacaacgtg cagtccatgg ccatcaaccg catgtggcaa tgacgctttc
     3181 cctgcatgca aaggggattg cattgcaaga aatcattttg gcagtcttta ctacgtgaat
     3241 ccggacgcgg gtaaggttct ttcagaatag cgcttccgca ttcaaccgtg caagtaatcg
     3301 cggtactctt tgtacaggtg agcgtccagt tcggccaggc gcgtttccag ttcctggatt
     3361 gcttcttcat tgtagtcgtg caggtaatga tgctgcaaca gaaccaggat gaagaagcag
     3421 atacgtgcgc gcactctctc cttgtatttt tcaaccacac gctctaaatc cgggctgagt
     3481 ctggccaggt ctttgctgat ttctttgact tcctccggca aaagtcttcg cagaggcagg
     3541 cgttgtgcca gagacatgag ctttgccgga aaccacacgg aggcaagttc tttgttctga
     3601 tctttttctt cgtagactcg cgtgaatacg tctgaagcag gatgagaggc gatgagagat
     3661 ggatctatga tgtccggttc tagaataaac gcatcgcgat agtagctgag cccttttgca
     3721 agagtctcgg gatctccggc tgcgctcgca catagggcct ctcccagtag aaagagaact
     3781 tcaggtgtgg atcgcacctt tcgtgcgaaa tgaagaatct ccgcagcgtt tgcataatcc
     3841 tcaatgcgaa tgaggcatct gcccaattcc gcaagagtgc gcgaatcatg ggtcagtcca
     3901 ctatcctgga acgataggcg gaattcttct gccgcgcgcc ccagaatgga cctcatcgca
     3961 gttcgaaagc tggaaagtcc cgagaattct ttgtcgtttg caagtgcctc aaactgatcc
     4021 cacgcttcca ccagcgcgct gccccgcgcc ttgcctggag gcgcttccag gatcatgctg
     4081 tggcgattgt cccagtatgc ggatgtgtaa aatccgcaga tgtagtccga ctcctccggg
     4141 tattcttcga gcagagagcc gtaaatgtct tttgctcgcg caaattcgcc cgcggcgaga
     4201 gcttcctggg cttcctgggc agatgtcatc taacggtagt tggaaaagct ggtgtcaaaa
     4261 tcgaagtttg cttctttgat catgcggatg attgcctgca gctcgtctct gtcctttccg
     4321 gttacacgaa cctggtcttc ctggattcgc gattgaacct ttagtttaga ttctttaata
     4381 attttggtga tcttcttgca ttgttcctga ttgagtccgc tttgaatttc cgttttgcat
     4441 ttaaactttc cgcttacatt ggattcaaac gggccgaact tgaacgcttt gaggctgatg
     4501 tttcttttta cgagcttgga ttgaatgaca tcaatgagtt gcttgagctt catttcatcc
     4561 tgggcttcca ggttcaacgt gtctttctca aggtcgatcg taacagggga ccctttgaaa
     4621 tcgaatctgt tttcaacttc tttgcgcact gcatcgagtg cgttggtcaa ttcctgacgg
     4681 tcaaccttgg agctgatatc gaaagaatat tccgtggcca tgcactctga tgacctcctg
     4741 gcatcaggag tgcaagcttt ctttcaacag acccttcgca cgctgcacgg cgctttcgag
     4801 gccggatgcg tccttgccgc cagcctgcgc catttctggt ttgccgcccc cgccgcctcc
     4861 tatgaggccg gaggcttcgc gaatgagttt accacagtca acaccggctt cgaccgcacc
     4921 ccggtttgcc atgaagatca gc
//
LOCUS       DAOQJL010000269         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10142_length_4917_cov_3.915878, whole genome shotgun sequence.
ACCESSION   DAOQJL010000269 DAOQJL010000000
VERSION     DAOQJL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10142_length_4917_cov_3.915878"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLG78_11865"
     CDS             complement(<1..184)
                     /locus_tag="PLG78_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23859.1"
                     /translation="MRLPVILLLICALSPALAADRLSKPLTDFAYMLDPSSTIEASEM
                     EAARDSGQFQRVKGDFI"
     gene            325..693
                     /locus_tag="PLG78_11870"
     CDS             325..693
                     /locus_tag="PLG78_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HNE23860.1"
                     /translation="MKKKFVMTVLALSAMVASPLLAKDMYGALAYSKADGAWGWWADA
                     TTQAAASKNALAKCSEHSSGCEVVLEFWNTCAAFATGSGGSYGWAHGPDENQVQSKAV
                     DFCSEHGSGCEVRVWACTSR"
     gene            792..1430
                     /locus_tag="PLG78_11875"
     CDS             792..1430
                     /locus_tag="PLG78_11875"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE23861.1"
                     /translation="MGIPGLKLKLTLQQKALAFIFAVVIGTSAITAYSSYMATHMVIE
                     NCKEGDLRTAATLIQNDMQEQSNKSLARATMVARMPVVINSMRTQSREPLVDALVPMF
                     LILREKYGVREGQFHLAPATSFLRIYDVKAGHGEDLSGFREMVLMANKNKLPYSGVEI
                     GRRGLSIRGIDVITDGTDYFGSVEIGMDFGAIINNVKKNAGFEAGVFVDDRK"
     gene            1427..1888
                     /locus_tag="PLG78_11880"
     CDS             1427..1888
                     /locus_tag="PLG78_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23862.1"
                     /translation="MSEIATLIPKPDAERIIGGYRNTESTDWNMIRTVVTPDFLRSVT
                     DVTTRQQTIGGVDYGFVAVPLLDFKGTKIGSVVAVRNFSEFATQNMWALVRSISLAFI
                     QGLILCGVMLILINSLFIHPEKENAEKRRQAALNKDADPGLIPGQSGDADD"
     gene            1881..2936
                     /locus_tag="PLG78_11885"
     CDS             1881..2936
                     /locus_tag="PLG78_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23863.1"
                     /translation="MIRTLFFAAAAFFLSGPLSAESVPTGTESPLRVFVSAIVSQIGK
                     VNDQAGNFEMTVDLQLRWRDPRLKFDAREHGSDRKAFLGDEAEAETKEIWTPQLVIRN
                     MNGSPIRLEQAITIQSDGTVVHLQRIKASFDTKFRLQAFPFDKQELPVRLISNKFNSN
                     QIVLTQDQTDKDTSGIKQGVGVSGFHLQKIAFSPGRERGLNGEYFPTMDARVQIARDP
                     TSHIFTLFAPFLALLVIPTVLTLFAKVDVAPRLTAWSASILALIALNFTFSIRYPAVG
                     ADGLINSVVSTGFGYQLLMVFISLIVLYPPVAEKITAKLGNKYVIAEVEKFMKWGIPV
                     GLFALLISSVLLTMYTT"
     gene            complement(2947..4512)
                     /locus_tag="PLG78_11890"
     CDS             complement(2947..4512)
                     /locus_tag="PLG78_11890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE23864.1"
                     /translation="MHIPRAFRGRLPTILFAILTYGAVYFLLHLLYNPIGQVLPRQWT
                     DSADQKVELPFYRLVDQPSTIRIKQKVIVPRGSALVLPRVSGNRILIFLNNQQVAAIG
                     AARGTGNIWTGFHWIDLPDVAGESVLELEITGAYDLGIREVPYLAPSGDIALFRWVTS
                     VLFNELYWLVLGLILALGALQIYYAQIDPRLRKTYWLNTAGILLGGFYLFDFAIRSDS
                     GTMTSYLWARKLMLSAGYAAPLLMLMAMEYYTRKKLYFSKFTLLTTAVAAGLAISAGS
                     LSDLKKWSVLTAGLAQLNWLFLLIIAFRTRHNVIVFAVTFFYCTLVGSVFSMISPSSH
                     IFMIQIGYVFAITSVVYNMIEEFGRVHEDYRTAYRKSRTDPLTGAYNRFILDEVTVEP
                     GDAVVLIDMNNFKVINDAFGHHAGDRVLVEWVKSAQLHTDQSDMIIRTGGDEFIILVR
                     GARETDMNRILEDFQQRVSDLPASFSYGIEKIQTTLHDAIQNADQRMYAHKKNSRSTW
                     IRRLWQRRQKKTA"
     gene            complement(4592..>4917)
                     /locus_tag="PLG78_11895"
     CDS             complement(4592..>4917)
                     /locus_tag="PLG78_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23865.1"
                     /translation="KLRSVIACRVDSLSKEEKEKIKAKLPSFVIPRSAALLSSFPYRN
                     PASYFVGPSKVYPAFQKELTADKIIPPVAIETYGSMSKPADQIGFAMPLESKRTDYAK
                     LMEAF"
BASE COUNT         1206 a         1167 c         1318 g         1226 t
ORIGIN      
        1 ctatgaaatc gccctttact ctttgaaact ggccgctgtc cctggcggct tccatttcgc
       61 ttgcctcgat ggtggacgat gggtcgagca tgtaggcaaa atcagtgagt ggcttgctca
      121 agcgatctgc tgcgagggca ggggacaggg cacagatcag aagcaggatc actggcagcc
      181 gcatatgtct gagtcgatgg gggctcctgg atcttacaat gtgaaaatcc accgccgggc
      241 ggtcgccaga gcgaaaaaac agttgaacaa aaacgcgaaa cgggctgtct ctcgttctgg
      301 gttcattccc acggaggagt tcgtttgaaa aagaaattcg ttatgacagt tctggctctc
      361 agtgctatgg ttgcttcccc acttcttgct aaagacatgt acggtgctct ggcttattcc
      421 aaagcagacg gtgcatgggg ctggtgggca gacgcaacca ctcaggctgc agcaagcaag
      481 aacgcgcttg cgaaatgttc agagcacagt tctggctgcg aagtggttct tgagttctgg
      541 aacacttgcg ccgcatttgc aactggcagt ggcggatcat atggctgggc acatggacca
      601 gatgagaatc aggttcagtc caaggctgtt gatttctgca gcgagcatgg ttctggctgc
      661 gaagtaagag tctgggcttg caccagtcgc tgacttaaag aaagggagga cgtccgtcct
      721 ccccgatttt tcatcgaact tccgggattt tagttgcgcg acccactccg tggggccatt
      781 cgaaccactt tatgggaatc ccaggcctca aactcaagct cactctacaa caaaaagcac
      841 tcgcattcat tttcgcagtc gtaattggaa ccagcgccat cacggcgtat tcatcctaca
      901 tggccactca catggtcatc gagaattgta aggaagggga ccttcgcaca gccgccactc
      961 tcattcaaaa tgacatgcag gagcaatcga acaagtctct tgcgcgcgcc acaatggtgg
     1021 cacgtatgcc cgtcgttatc aattccatgc gaacgcagag tagagagcca ctggtggacg
     1081 cgctggttcc tatgtttctc atcctgagag aaaaatacgg agtgcgcgaa ggccagtttc
     1141 atctggctcc cgcgacttct tttctccgga tctatgacgt aaaggccggt cacggggaag
     1201 acctcagcgg ctttcgcgaa atggttctca tggcaaacaa aaacaaactg ccgtattcag
     1261 gagtcgagat tggaagacgc gggctcagca ttcgcggcat tgacgtgatc acggacggaa
     1321 cggactactt tggcagcgtg gaaatcggaa tggatttcgg agccatcatc aacaacgtaa
     1381 agaagaacgc gggttttgaa gcgggcgtgt ttgtggatga tagaaaatga gcgaaattgc
     1441 caccctgatt ccgaagccgg atgcggagag aatcattggc ggatatcgaa atacggaatc
     1501 cactgactgg aatatgattc gcactgtggt cactccagac tttctgcggt ctgtgacaga
     1561 tgtgaccacg agacagcaga cgataggcgg cgttgattat ggctttgtgg ctgttcctct
     1621 ccttgatttt aagggaacaa agatcggttc tgttgtcgcg gttcgcaatt tttcagagtt
     1681 cgcaactcaa aacatgtggg ccctcgtgcg ttccatctct cttgctttca ttcaagggct
     1741 gattttgtgc ggagtcatgc tgatcctgat caactctctc ttcatccatc ctgagaaaga
     1801 gaatgcggag aaaagacgcc aggccgctct aaacaaagat gcagatccag ggctcattcc
     1861 tggacagagc ggagacgcag atgattagga ctctcttttt cgccgcggct gcattcttcc
     1921 tgtctggccc gctctctgcg gaaagcgtcc ctacaggaac agagtcacct ctccgcgttt
     1981 ttgtttctgc cattgtaagt cagattggaa aggtcaatga tcaggccgga aactttgaaa
     2041 tgaccgtcga tctgcagctg cgatggcgag atcctcgtct caaatttgat gccagagaac
     2101 atggctcaga tagaaaggct tttctgggag atgaggcaga agcggaaaca aaagagatct
     2161 ggacccctca actggtgatt cgcaatatga atggatctcc cattcgtctt gagcaggcga
     2221 ttacgattca atcggatggc actgtagtgc atttgcaaag gatcaaagct agttttgata
     2281 ctaagtttcg cctgcaggcc tttccgtttg ataagcagga gcttccggtt cggctcatat
     2341 ccaacaagtt caatagcaat caaatcgttc tgactcagga tcaaacggac aaagacacat
     2401 ctggaatcaa gcagggtgtg ggagtgtcgg ggtttcactt gcagaagata gccttttctc
     2461 ccggccgcga gagaggactc aacggtgagt atttcccaac gatggatgcg cgcgttcaga
     2521 tcgcgcgtga tcctacttct cacatattca ctctgtttgc tccttttctg gcacttctcg
     2581 taattccaac cgtgctgact ctgtttgcga aagtggatgt tgcgccaagg ttgactgcat
     2641 ggtccgcgtc tatcctggcg ttgattgcgt tgaacttcac attctccatt cgttacccgg
     2701 ccgtgggcgc ggatggtctg atcaattcag ttgtttccac tggatttggt tatcagcttc
     2761 tgatggtttt cattagcttg attgtactct atccaccggt ggcagaaaag atcacagcaa
     2821 agcttgggaa caagtacgtg attgcggaag tggaaaagtt catgaagtgg ggcattccgg
     2881 tgggtttgtt tgcgctattg atcagcagcg tgcttttgac catgtatacg acttgatgtt
     2941 ttgaactcag gcagtctttt tctgcctgcg ttgccagagt cttcgaatcc aggtggacct
     3001 ggaattcttt ttatgcgcat acattctctg gtccgcgttt tgaatggcat cgtgcagagt
     3061 ggtttgtatc ttttcgattc cgtaggagaa agacgcaggc aaatctgaaa ctcgctgctg
     3121 gaaatcttcc agaatgcgat tcatgtctgt ctctcttgct cctcgcacta gaatgatgaa
     3181 ttcatctccg ccggtgcgaa tgatcatatc gctttgatct gtgtgcagtt gcgcgctctt
     3241 cacccactct acaagcacgc gatctcctgc gtgatgtccg aatgcatcgt tgatcacttt
     3301 gaaattgttc atgtcaatga gaacgactgc gtctcctggt tctaccgtca cctcgtcgag
     3361 tatgaatcga ttgtaggcgc ctgtgagagg atctgttctt gattttctat atgcagtgcg
     3421 ataatcctca tgcactcgcc cgaattcttc tatcatgttg tacacaacag atgtaatcgc
     3481 aaagacgtat cctatctgga tcataaaaat gtgagaagat ggtgagatca tagagaagac
     3541 acttccgacc agagtgcaat agaagaaagt gactgcgaaa acgattacgt tgtgacgagt
     3601 gcgaaacgca atgatcaaga gaaaaagcca gttgagctgg gcaagccccg ccgtaagcac
     3661 actccatttc ttcagatcag aaagagatcc ggccgaaatc gcaagccctg cagccaccgc
     3721 agttgtcaaa agagtgaact ttgagaagta aagcttcttg cgagtgtagt actccattgc
     3781 catgagcatc agaagaggcg ccgcatatcc cgcagaaagc atgagttttc tggcccagag
     3841 gtaggaggtc atggtgccgc tgtcgcttcg aatcgcgaag tcgaacagat agaagccgcc
     3901 aagcaaaatt cctgcggtgt tgagccagta ggtttttcgc agccgcgggt caatctgcgc
     3961 gtagtagatt tgaagcgccc ccagggcgag gataagcccc aggaccagcc agtagagttc
     4021 gttgaacagg acggaagtga cccatcggaa cagagcaatg tcgcctgacg gggccaggta
     4081 tggaacttct ctgattccca ggtcgtatgc cccggtaatc tccagctcca gaacggattc
     4141 tccagccacg tccggcaagt cgatccagtg aaatccagtc cagatgtttc cggttccgcg
     4201 cgcggcacca atcgcagcta cctgttgatt gtttaaaaat attagtattc ggtttccaga
     4261 tacacgcggg agaaccagag cagaaccgcg gggtacaatt actttctgtt tgattctgat
     4321 ggtggatggc tgatctacaa ggcgatagaa tggcagttcg actttctggt cagcagaatc
     4381 agtccactgg cgcggcaaaa cttgtccgat cggattgtag agcaaatgca aaagaaaata
     4441 caccgcacca taggtcagaa ttgcaaagag gatcgtgggg aggcgtcctc gaaaggcgcg
     4501 cggtatgtgc atgcggcagg atgatgcaaa ggttcaaaaa gggacaagca gatttgtaga
     4561 cctgtatcca cggggcgaat ccggcgcaga gttagaaagc ttccattagc tttgcgtaat
     4621 cggtacgctt gctttcgaga ggcatggcaa aaccgatttg atctgccggt ttagacatgc
     4681 tgccgtacgt ttcgatggcc actggcggaa tgatcttgtc tgcggtgagt tctttttgaa
     4741 aggccgggta tactttcgaa gggccaacga agtaggaggc cggattgcgg tatggaaaag
     4801 acgagaggag tgcggcggag cgcggaatca caaaggatgg cagtttcgct ttgatcttct
     4861 ctttctcttc ctttgaaaga gaatctacgc ggcaggcaat cacagaccga agctttt
//
LOCUS       DAOQJL010000270         4909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10159_length_4909_cov_3.775237, whole genome shotgun sequence.
ACCESSION   DAOQJL010000270 DAOQJL010000000
VERSION     DAOQJL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_4909_cov_3.775237"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLG78_11900"
     CDS             <1..1320
                     /locus_tag="PLG78_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE23866.1"
                     /translation="GESKAKPKSKEEILADLNKLIGLTSVKGAISDLITFLDVQRKKQ
                     DAGLKADRIPLHSVFLGNPGTGKTTVARILADVFKSLGYIEKGHLVEVDSSDLIGGYV
                     GQSEEKTKAILDKAMGGVLLIDEAYALAKSEFGKQAINIILKRMEDDRDKFVLIATGY
                     EKEMEEFFATNTGLKSRFSRYFTFADYDPGQLLQIAQGIASDSQLNLTDDAKSEIAAI
                     VQREYTNRKAGFGNARFVRQLLDEIKLAQSSRIMLLERAGNLVSLDMLQIVAPEDVRS
                     TGYYSDASSGTPKRQQQNEIENEIFKICLKIPADLQTLFLRNIQSISYYIAMSDGNFS
                     DEEAQFIRALFQNLSAKSSLFQPIANEMIDLPTTKRLLEEASKGNNGKMTVSLPNPER
                     VSQDDLMLFKTALFRTGVLVAEAEGGINEQEKKALANLRKMVGLANK"
     gene            1345..1500
                     /locus_tag="PLG78_11905"
     CDS             1345..1500
                     /locus_tag="PLG78_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23867.1"
                     /translation="MPVLIQLWCIVLRDFAWQYLLLERKLEFAAKRVLINLRALLRLE
                     HKNGKVK"
     gene            1776..2159
                     /locus_tag="PLG78_11910"
     CDS             1776..2159
                     /locus_tag="PLG78_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23868.1"
                     /translation="MTIANSFSISEAFQGATLARGEDFEIFKGVASAGSLYQVLYDHN
                     RPGFLIVLEGCDALLKNEAAINILQGVLKPEDAVAAFSAPKMPQDRDGDTIPAKFEMV
                     AGVIFVSQSSLDELPVVLKSRVLEP"
     gene            2175..2786
                     /locus_tag="PLG78_11915"
     CDS             2175..2786
                     /locus_tag="PLG78_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23869.1"
                     /translation="MAKNSKAFLKAIRARKGARAYQGADPSRADLIFVGIEANWPKVA
                     ELSSAHRDAIIDYLSDGAAFWTRHGFHHPLLCCGGRVPGRRFHTRFAKMHLDASYANR
                     VAFVELLAKHTTRGSSWGAATLMRAVSSKQNAPHLKQLQIWLQGSEKIVFLGSALLRD
                     SRIRESLGIQNPTGRNVIEHTHLSRISNKNLGQLRKKILRLGG"
     gene            2790..3575
                     /locus_tag="PLG78_11920"
     CDS             2790..3575
                     /locus_tag="PLG78_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE23870.1"
                     /translation="MENSEAFQEQSDSKKFSKHGARECAKCGWRGDSRFCQNCGTEII
                     EEFCTNCGAQLTTSFCTSCGVARETLQGGSTSFAGTEAFDSEQAIHSGEVERGSENAA
                     QSAEDDAVAAFERACFEFGEQISGTWHWRYTTSLGTPMLCLLTIRVFGGEADADAFFL
                     TIGDETQFPSNCVRRVFYDLTKGSPIQVVTEAISYCSPAGISTDVVGMVMLSDGYTAW
                     ENEKVGAANEYEYDDELDILQCTGAVYERMPRSRVTTIMQGLF"
     gene            3575..4186
                     /locus_tag="PLG78_11925"
     CDS             3575..4186
                     /locus_tag="PLG78_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23871.1"
                     /translation="MNEIQVTLTGNMIAFWALEFAGLECQKIRDYCEEHDEAIETFFG
                     GPSEEESVQAALEDDNWDLFSAAAVTDDFGSGGNAFIADEDLSIIVTHASDRIEIPFT
                     HLRQANRRTVSAEDWSASRAGQRILYIFGYRSDSPSAAEFSLGRSDMFSIDRLAIAVC
                     ENQIIGTSFVASISYTHPDGAVTVDATGNDFDGEYFPAEFLQT"
     gene            4260..>4909
                     /locus_tag="PLG78_11930"
     CDS             4260..>4909
                     /locus_tag="PLG78_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23872.1"
                     /translation="MKSISRAAKIHLLSAWAIMASIACVSQEMQNAQEICQKRDQSPV
                     QCAAQNFKEGVGMEDSGYYGSAKSAFRIAINLDPEGKNTWRYYAELCAVNYPLGRRAI
                     KHGKQFQWDDLHREVGYDRWLEEALAECDAAVAWAKDEYPDALAARGSLRLLAENKSG
                     ACEDFRKAKSIGGGEEPRIYTHDLVTACGSPTYFAFGPPQALRVDRDHLQIILPTS"
BASE COUNT         1302 a         1143 c         1334 g         1130 t
ORIGIN      
        1 ggagaaagca aggccaagcc aaaatcaaaa gaagaaatac tggcggactt gaataaacta
       61 ataggactca catctgtcaa aggagctatc tctgatctaa tcacgtttct tgatgtgcag
      121 aggaaaaagc aagatgccgg cttgaaggct gaccgcatac cactgcattc tgtttttcta
      181 ggcaaccccg ggacgggcaa gacgacagtt gcccgaatcc ttgcggacgt gtttaagtcc
      241 cttggttaca tcgaaaaagg acaccttgtc gaggtcgatt catccgactt gatcggtgga
      301 tatgtcggtc aatctgaaga gaaaacgaag gcgattctcg acaaggcaat gggaggagtc
      361 ctcctcatcg atgaggcgta tgcgctggca aagagtgaat ttggcaagca agcgattaac
      421 attatattga aacgaatgga agatgatcgg gataaatttg tcctgattgc cacgggttac
      481 gaaaaagaaa tggaagagtt ctttgcaacg aacacaggat tgaaatcacg tttcagtcgc
      541 tatttcactt ttgcagatta tgatcccgga cagttattgc aaattgcgca aggcatagcg
      601 tcggattcgc aattaaacct gacggatgac gccaaatcgg aaattgccgc aatcgttcaa
      661 agagaataca cgaatcggaa ggcaggtttc ggcaatgcga ggttcgtgcg tcagttacta
      721 gacgaaatca agctagctca atcgagcagg atcatgttac ttgagagagc cgggaattta
      781 gtgagcttgg acatgctcca gattgttgct cctgaagatg tgcgaagcac cggatactac
      841 tccgatgcat caagcggcac accgaagagg caacagcaga atgagatcga aaatgagata
      901 ttcaaaatat gcctcaaaat tccggcagac cttcaaacgc tctttttaag aaatatccag
      961 tcgatttcct attacatcgc aatgagcgac ggcaattttt cagatgaaga agcccagttt
     1021 atccgcgcgc tattccaaaa cttgagcgca aaaagctcgc tctttcaacc gattgcgaat
     1081 gaaatgatcg accttccgac caccaaacgg cttttggaag aggcgtcaaa aggcaacaac
     1141 ggcaaaatga cggtaagcct gccaaatcca gagcgcgtgt cgcaggatga cctgatgcta
     1201 ttcaaaacgg ctctctttcg gaccggggta ctcgtagcgg aggccgaagg tggtatcaac
     1261 gaacaggaaa agaaagcatt ggccaatcta cgtaagatgg tgggacttgc gaacaagtag
     1321 gtaggaggat gcgacgctgg gagaatgcct gtcttgatac agttgtggtg catcgttcta
     1381 cgagatttcg cctggcaata cttgctgctg gagcggaagc tggagtttgc ggcgaagcgc
     1441 gttctcatca atctgcgcgc tcttctcagg ctcgaacata aaaacggaaa ggtgaaatga
     1501 tgcaatgctc aaattgtcaa tcggagaatg atgcggacgc gaagtactgc gcggcatgtg
     1561 gccaggtttt gaagtttgtt tgcctacatt gcgggaccga gagtagtggc aagttttgcg
     1621 gaaagtgcgg aaaaccgtta ccaaatgccg tcgcaacagg gcaaactggc tccaaggaga
     1681 atcacgagaa cgcaggagag aaactgagac aagtaggaac gtatgcgaag cttgtgtgtg
     1741 aaggtagcaa gccgggatgt atatttgccg cacctatgac tatcgccaat tcattctcca
     1801 tttctgaggc ctttcaaggt gcaacgttag cccgcggaga agacttcgaa atctttaagg
     1861 gcgttgcgtc tgccgggagt ctgtaccaag ttctctatga ccacaataga ccaggctttc
     1921 taattgtcct cgaaggatgc gacgccctcc tcaagaatga agcagcaata aatattctcc
     1981 aaggtgttct caaaccggag gacgcagtag ccgcgttttc agctcctaaa atgccgcagg
     2041 acagggatgg cgacacgatt ccagccaaat tcgaaatggt ggctggagtt atttttgtct
     2101 cgcaaagctc ccttgatgag ttgccagttg tgcttaagtc aagggtattg gaaccttgag
     2161 gattctcggg cgctatggca aagaactcta aagccttttt gaaagcaatc cgcgcgcgaa
     2221 aaggggcacg cgcctaccag ggagccgatc cgagccgcgc ggacttgata ttcgttggta
     2281 tcgaagcaaa ttggcccaag gtcgcagaac tgagttccgc gcatcgcgat gcaattattg
     2341 actatctttc tgacggcgcc gcgttttgga caaggcacgg gttccaccat ccattgttgt
     2401 gctgcggcgg acgcgtgccg gggcgccggt tccatactcg cttcgcaaag atgcacttgg
     2461 atgctagcta cgcaaatcgc gtggcgtttg tggagctttt agcgaagcac acaacacgcg
     2521 gaagttcttg gggggccgcg accctcatgc gggcagtctc tagtaaacaa aatgcgccac
     2581 atttgaagca attgcaaatc tggctccaag ggtctgagaa aatagtcttc ctcggtagcg
     2641 cactcttgcg ggattctcgt atccgcgaaa gtctgggtat ccagaatccg accggaagaa
     2701 acgtgattga acacacccac ctctcaagga ttagcaacaa gaaccttggg caattgcgaa
     2761 agaagattct caggttaggc gggtgatcca tggaaaactc tgaagctttt caagagcaat
     2821 cggattcgaa gaagttttcg aagcatggag caagagaatg tgcgaaatgt ggttggcgcg
     2881 gtgattcacg gttctgtcag aattgcggga ctgaaataat tgaagaattc tgcacaaatt
     2941 gtggtgcgca actgaccact tcattctgca ctagttgcgg tgtagcgcgc gaaacccttc
     3001 aagggggcag cacttcgttt gcaggcactg aagcttttga ctcagaacag gcaattcatt
     3061 cgggtgaagt ggagcgaggg agtgagaacg cggctcaatc cgctgaagac gatgctgttg
     3121 ctgcctttga gcgcgcatgc tttgagttcg gagaacagat ttccggtacc tggcattggc
     3181 ggtatacgac gagtcttggg acgcccatgc tctgtttgct caccattcgc gtttttggcg
     3241 gggaagctga tgctgacgcc ttcttcctta ctatcgggga cgaaacacag ttcccttcca
     3301 attgtgtgcg gcgtgtgttt tacgacctga cgaaaggttc acccatccag gtcgtgacag
     3361 aagcgatctc gtattgctcc ccggcaggca tttcaaccga tgtggtcggt atggtaatgt
     3421 tatctgacgg ctacaccgca tgggaaaacg aaaaagtcgg ggcagctaac gagtacgaat
     3481 acgatgatga acttgatatc ctgcaatgca ccggcgcggt atacgagcgc atgccaagat
     3541 ccagagttac tacgattatg caggggttgt tctgatgaac gaaattcagg tcacgctaac
     3601 gggcaacatg attgcgttct gggcccttga gtttgctgga ctagaatgcc agaaaatacg
     3661 agattactgc gaagagcatg atgaagccat cgaaactttc tttggtggcc cgagtgagga
     3721 ggagtcagtc caagcagcgc tcgaagatga caattgggat cttttcagtg ccgctgccgt
     3781 gacagacgat tttgggtccg gcggaaacgc tttcatcgcg gatgaagatc tttcgatcat
     3841 cgtgacccac gcgtcagatc gcattgaaat tcccttcacg catttgcgcc aggcgaaccg
     3901 tagaacagtt tctgcggaag attggagcgc gtcgcgcgcc ggtcaacgga ttctctatat
     3961 attcggatat cgctccgatt caccttccgc ggcagagttc tcattgggcc gcagcgatat
     4021 gttttccatt gacaggctgg cgatcgcggt gtgtgaaaat cagatcatcg gaacatcatt
     4081 cgtcgcaagc atttcttata cccatccgga cggcgccgtg accgtggacg caacgggcaa
     4141 tgattttgac ggtgagtatt ttccggctga atttctgcag acttaagatg ttgccgccga
     4201 cccttcgaga ctaccgatat caaattgacg aagccgtcaa ccgttcgcac cacggtggaa
     4261 tgaaatctat ctcccgagcg gcaaaaattc atcttctgag tgcatgggcc atcatggcca
     4321 gcatcgcatg cgtctcgcaa gaaatgcaaa atgcccaaga gatttgccag aaaagggatc
     4381 aaagcccggt gcaatgtgca gcacaaaatt tcaaggaggg tgtcggaatg gaggattcag
     4441 gctactacgg ttcagcaaaa tcagctttcc ggatagccat caatctggat ccggagggaa
     4501 agaacacgtg gcgttattat gctgaacttt gcgcggtaaa ctacccattg ggtaggcgag
     4561 cgattaagca cggcaaacag ttccaatggg acgatctcca ccgagaagtc ggatatgatc
     4621 ggtggttaga agaagctcta gcggaatgtg acgccgcggt agcctgggcg aaagatgagt
     4681 atcccgatgc tcttgccgct cgcggatctt tgcgcctcct agccgagaat aagagcggcg
     4741 cctgcgaaga cttccgcaaa gccaaatcaa ttggcggagg ggaagagcca agaatctaca
     4801 cgcatgatct tgtaactgct tgcggatctc caacctactt tgcgtttgga ccgcctcagg
     4861 ccctgcgcgt cgatcgtgac catttacaga tcatcttacc gacatcgtt
//
LOCUS       DAOQJL010000271         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10214_length_4889_cov_4.742036, whole genome shotgun sequence.
ACCESSION   DAOQJL010000271 DAOQJL010000000
VERSION     DAOQJL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10214_length_4889_cov_4.742036"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLG78_11935"
     CDS             <1..304
                     /locus_tag="PLG78_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23873.1"
                     /translation="VAIPAFLLHQNIAYGSALGEFITYGLRAYLFGFAIWWASWGIGV
                     ALFAAALYVIVETLTILMISVRATYAIQMRGTLETIARVVLYAGLPVWVLFRIFSA"
     gene            complement(312..2981)
                     /locus_tag="PLG78_11940"
     CDS             complement(312..2981)
                     /locus_tag="PLG78_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE23874.1"
                     /translation="MSDWEFRAGVPTQPLDPQGAVWTKTHLPSYGKPVQGFDQYRGWL
                     LFRSRLPSELTAQNGDQLAVDAGLLSDVARVYVNDSLAGELGNVDPYSTGSMVTFVRT
                     VPNSIRLKSDGNYLYLALYTDGRYPIFASENPRVGAAEDILRDYYQSELLSIALLGVY
                     LLVGLYYLFLWSRRTKEVYHLFFGLLCVLLCFYWFFRLGSRDVVFGNHALLRSKVEYS
                     LLFLISPIFSFFLSQFFHGKYDRVGLLLTPFYALLLIVTAFGSYSVAVMCLTIWQFCI
                     MPIIAYYIFYIVREVYRRNPSALYLVPGLVALSLGAVHDILAIRGWISTPMIARFAFP
                     VFILGGALVLTHRFVFVHTQVEELLGNAYRLENFRTELLSIEPGASITELLERILVVL
                     ESLFECEKSFAIVPDAFGALHFTQELPEEIRNLILKGSRLHRRTKLISAEFFETYGSV
                     LQMELLKLEPSSPEGKQHLVSLRATELVFQKVAELGHKIAIALPHESEVLGMVFLGAK
                     KNKEKYSEAELSLIKTFQYSITQAIRNKMLFSEISQLKGEAEERVEKLSEYVIDRRNS
                     VQMEVKEKTIVYSSPSMVDVYEQAKKYADANKPVLISGETGTGKELIARAVHHFRNPD
                     APFIAVNCAAIPENLWESEVFGHARGAFTSAHGTHAGLIERAKGGTLFLDEIGEMPIS
                     VQPKMLRLLQERKFSRVGGEDELPASCQFLFATNRSLLDMQREGRFREDLYYRINVFQ
                     IHIPPLRDRRAEVPDLVRFFLAQYSRELRGRDTTVEEPALSALCRYSWPGNVRELENC
                     MIQALVHCKGEVLEVEDLPPVIRTFTQTRLAAEPPVQFKTAVSFDMLLADYSRKLILA
                     AMSRVKGNKLQTAKLLGLKRTTLYNKMKELGLR"
     gene            complement(3155..4849)
                     /locus_tag="PLG78_11945"
     CDS             complement(3155..4849)
                     /locus_tag="PLG78_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE23875.1"
                     /translation="MENANLAFDYVIIGSGFGGSVSAMRLAQKGYSVAVLEAGKRWEG
                     KDFPKTNWSLRKFLWMPKAFCYGIQRINVLNDVMILSGAGVGGGSLVYANTLYVPKKE
                     VFEYPLMKKMGGQRHMLPFFQIAQKMLGVITNPALTEVDELMKQTAAELGKGDTFTPT
                     PVGIYFGEAGKTVKDPYFGGEGPDRTGCNFCGGCMVGCRFDAKNTLDKNYLYFAEKLG
                     VKVFPETKAVDIIPLSADGSAGYEIRTKRTTGFFGLPNRTFRAKGIVFSAGVLGTMKL
                     LLTMQETGRLTRLSGRVGHMVRTNSEAIVGATSLSSKVDYSKGIAITSSVHPEEHTHI
                     EPVRYSRGSDAMGSLASVLTDGGGKIPRQIRFLGNVIRHPIQFLRTIHPIGFAKKSII
                     LLVMQTLDNHIHIVNKRRLIWPFSKSLSSEQEDAHDRKIPTYIPMANEFARRLAKRIG
                     GIPRSSYNEVLLNIPTTAHILGGACMGETPEDGVVDTQNRVFGYQNMLVCDGSMVPVN
                     LGVNPSLTITAFTERAMSFIPPKEKSVQAFKFEKKWGITSRLTENVKKGGFRLKFW"
BASE COUNT         1269 a         1244 c         1156 g         1220 t
ORIGIN      
        1 cgtggcgatt cctgcatttc tacttcacca gaacatcgcc tatggaagcg ctctgggaga
       61 attcatcacc tatggcttga gggcatacct gttcggattt gcaatctggt gggcatcgtg
      121 gggaatcgga gtggcactct ttgcggcagc gttatacgtc attgtggaaa ctttaacaat
      181 ccttatgatt tcagtccgag ctacatacgc gatccaaatg cgcggcaccc ttgagacgat
      241 tgcgcgcgtt gtactctacg ccggcctgcc tgtgtgggtg ttgtttcgaa tcttctcagc
      301 gtgattcgcg attaccgcag tccaagctct ttcattttgt tgtagagcgt ggttcgtttc
      361 aatccaagaa gcttcgcagt ctggagtttg ttccctttca ctctggacat ggctgctaga
      421 atcaacttgc gcgaatagtc ggcgagtaac atgtcaaagg atacagcagt cttgaactga
      481 accggcggtt ctgccgcaag acgcgtttgc gtgaaggtcc tgatgacagg cggcagatct
      541 tccacctcca gcacttctcc cttgcaatga accagagcct gaatcatgca attctcaagc
      601 tctcgcacgt tgcctggcca ggaatacctg cacagagcgg agagagccgg ctcttccact
      661 gtagtatcgc gtcctctcaa ttcgcgcgaa tattgagcca ggaagaatcg caccagatct
      721 ggaacctccg cacgacgatc gcgaagagga ggaatgtgaa tttgaaatac attgattctg
      781 tagtaaaggt cttcgcgaaa tctcccttct ctctgcatat ccagaaggct cctgttcgtt
      841 gcaaacaaga actgacacga tgcaggtagt tcatcttctc cacctactct ggaaaatttt
      901 ctctcttgta aaaggcggag catctttggc tgaacggaaa tcggcatttc tccaatttcg
      961 tccagaaaga gagttcctcc ttttgctctc tcaatgagac ccgcatgggt tccatgcgcg
     1021 ctcgtaaaag cacctctcgc atgcccaaac acctcactct cccacagatt ctcgggaatg
     1081 gcagcgcagt tcaccgcaat gaaaggagca tccgggtttc tgaagtgatg aacagcacgt
     1141 gcgatgagct ctttgcccgt tcctgtttct ccggaaatca gaactggctt gtttgcgtcc
     1201 gcgtacttct ttgcctgttc gtatacgtcg accatggatg gactcgaata gacaatggtt
     1261 ttctctttca cttccatctg aaccgagttc ctgcgatcga tgacgtattc ggaaagtttc
     1321 tccactcgtt cttccgcctc tcctttcagt tgagagatct cagaaaaaag cattttgttg
     1381 cgaatggcct gcgtgataga gtattgaaag gttttgatga gagacaattc cgcttccgaa
     1441 tacttttctt tgtttttctt cgcgccaaga aacaccatcc cgagtacttc tgattcgtgc
     1501 ggaagtgcta tggcaatctt gtgtcccagt tctgctactt tctgaaacac aagctctgtg
     1561 gctcttagac tgaccagatg ctgcttccct tctggagaag aaggctcgag tttcaggagt
     1621 tccatttgaa gaacagatcc gtatgtctcg aaaaactcag cggaaataag ttttgtgcgg
     1681 cgatgcaacc gactcccttt caaaatcagg tttctgattt cttctggcag ttcctgggtg
     1741 aaatgaaggg caccaaaggc atccggaaca atggcgaatg acttctcgca ttcgaataaa
     1801 ctctcaagga caacaagaat tctttccagg agctctgtga tgctagcgcc tggttctata
     1861 gacaaaagct cagtgcgaaa attttcaagg cgatatgcat ttccaagaag ctcttcgacc
     1921 tgcgtgtgaa caaaaacgaa tctgtgagtg aggaccagag ccccgcccag aatgaaaact
     1981 ggaaaggcaa agcgcgcgat cataggagtg gatatccagc ctcgaatcgc caggatgtca
     2041 tgcacagcac ccagactcag cgccacgaga ccaggaacca gatacaatgc acttggattt
     2101 cttctgtaaa cctcgcgcac tatatagaag atatagtaag caatgatagg catgatgcag
     2161 aattgccata tggtgaggca catcactgca acactatagg atccaaatgc agtgacgatc
     2221 agcagaagag catagaatgg agtcagaagc aagccgaccc tgtcgtattt gccgtggaaa
     2281 aactgggaca aaaagaaaga aaatatggga gatattaaaa aaagtaaact atattctacc
     2341 ttcgatcgca acaatgcgtg atttccaaac actacatcgc ggctgcccag cctgaaaaac
     2401 cagtagaaac agagcaaaac acaaagcaga ccaaagaaca gatggtagac ttctttagtt
     2461 cttctgctcc atagaaatag atagtacagg cccacaagca aatagacacc gagaagagca
     2521 atcgacagca gttcgctctg gtagtagtct cgcagaatat cttctgcagc gccaaccctt
     2581 ggattttcag aagcgaagat cggataacgt ccatccgtgt agagagcaag gtaaagatag
     2641 ttgccatctg atttcaaacg aatcgaattc ggaacagttc tgacgaacgt caccatcgat
     2701 ccggtgctgt aaggatctac attgcccaac tcgcctgcca gcgaatcgtt tacgtatacg
     2761 cgcgccacat cagacaataa tccagcgtcc acagcaagct gatccccatt ttgcgccgtg
     2821 agttctgatg gaaggcgcga acgaaacaaa agccatccgc gatattgatc gaagccctga
     2881 actggcttac cgtaagaggg aagatgagtc ttcgtccaga ctgcgccctg tggatcgagg
     2941 ggttgagtgg gaacgcctgc gcgaaattcc caatcgctca atgtaaagat tttagcgtcc
     3001 tgtgcgcagc ccgtctgaaa aagacaggtc aatacgaaaa tccaaacgca tctatgccgg
     3061 gtcatgtgat tcgcaatcta cgatatcagt agtcgcgcat ctggtccagc gcaaaccctg
     3121 gaaaggcgaa attgtactgc gtgcaacagt agagttacca gaacttgagt cgaaatccgc
     3181 ccttcttcac gttttctgtc agacgcgatg tgattcccca cttcttctca aacttgaacg
     3241 cctgcaccga tttttcctta ggcggaatga aactcatcgc gcgctctgtg aacgcagtga
     3301 tggtcaaact tggatttaca ccaagattca ctggaaccat agagccatcg catacaagca
     3361 tgttctgata accgaacaca cgattctgtg tatctaccac gccgtcttcc ggagtttctc
     3421 ccatgcatgc accaccgagt atatgagcag tggttggaat gttcaaaagt acttcattgt
     3481 aagaactgcg ggggattcct ccaattctct ttgcgagacg acgcgcaaac tcattggcca
     3541 ttggaatgta tgtaggaatc ttacgatcat gcgcgtcttc ctgttcagaa gaaaggctct
     3601 tgctgaaagg ccagatcaaa cgacgtttgt tcacaatatg aatgtgattg tcgagagtct
     3661 gcatgaccag aagaatgatg gacttctttg caaatccgat tggatggatg gttcgcagaa
     3721 actgaattgg atggcgaatc acgtttccaa gaaaacgaat ctggcgtgga atctttccac
     3781 ctccgtctgt cagtacactt gcaagagatc ccatcgcatc cgaaccgcga gaatagcgca
     3841 caggttcgat gtgagtgtgc tcttccggat gaacgctcga cgtgatcgca attccctttg
     3901 agtaatccac tttcgaagaa agcgaagttg ctcctacgat cgcctcgctg tttgtgcgaa
     3961 ccatatggcc cactctaccc gaaaggcgag tgaggcggcc ggtttcctgc atggtcaaaa
     4021 gaagtttcat ggttccaaga acgcctgcag agaacacgat tccctttgcg cgaaatgtgc
     4081 gattggggag tccaaagaat ccagtcgtgc gctttgtgcg aatttcgtaa ccggcagatc
     4141 catccgcaga aagcggaatg atatctactg cttttgtttc cgggaatact tttacgccca
     4201 gcttctctgc gaaatataaa tagttcttat ccagagtgtt cttcgcgtcg aatctacagc
     4261 cgaccatgca tccaccgcag aaattgcagc cagtgcgatc cgggccttct ccgccaaaat
     4321 acggatcctt cacagtcttg cccgcttccc cgaagtaaat tccaacagga gtgggtgtaa
     4381 acgtatctcc ttttccaagc tccgccgcag tctgcttcat gagttcgtcc acttctgtga
     4441 gcgcaggatt ggtgatcact ccgagcatct tttgtgcgat ttgaaagaat ggcaacatgt
     4501 gacgctgccc gcccatcttc ttcatcaaag gatattcgaa gacttccttc ttgggcacat
     4561 atagagtgtt ggcatacacc agactgcctc cgccgactcc ggcgccgctc aggatcatga
     4621 cgtcgttgag aacattgatg cgctggattc cgtagcagaa cgctttgggc atccagagga
     4681 atttgcgaag gctccagttt gttttgggga agtctttgcc ttcccagcgc ttgcccgctt
     4741 cgagcaccgc cacagagtag cccttctggg ccagtcgcat cgcggaaacg cttcctccaa
     4801 aaccagaacc aatgattacg tagtcgaatg ctaaattcgc attttccatc ggcagagtcc
     4861 aaactcggcg gccggagtgt ccatccaaa
//
LOCUS       DAOQJL010000272         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10219_length_4887_cov_4.546978, whole genome shotgun sequence.
ACCESSION   DAOQJL010000272 DAOQJL010000000
VERSION     DAOQJL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10219_length_4887_cov_4.546978"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1139
                     /locus_tag="PLG78_11950"
     CDS             51..1139
                     /locus_tag="PLG78_11950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE23876.1"
                     /translation="MKARLVLVCFVATCALWYLLPNPGSAEETAIPSYPAANPGKATI
                     LFGGDTHFQWGIEELIEKEDALAPVREMQRTFEKADFRILNVETAITDKGEPASGRSH
                     IFRASPGSIKTLTPLKLNLAILANNHAMDMGPEGLEDTRQNLRKAGILTAGVGANAKE
                     ASLPVLFRIGDIRFAVVAASAIGDERMWADDKTPGIAKPEQILAQIQAARTLADHVIV
                     SLHWGMEYNPYPMPDQRPLAQQFIQAGASAVIGHHPHVAQGIETSKNTVICYSLGNFL
                     FGSSNPFQTDNMIVLMEFEAKKPGVRGLRVIPITGHYRESGHAPRPLHTTELHSFFPD
                     LWTQSTKINPSMSSRIIEEEDGIRINVD"
     gene            complement(1160..2203)
                     /locus_tag="PLG78_11955"
     CDS             complement(1160..2203)
                     /locus_tag="PLG78_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23877.1"
                     /translation="MQIILLLVFAVPLFAETPIQNVSPATPTTPAKPDSRPSKEARTR
                     AETDLELKRLTASLDAAFRLQKKFEIPGLLEDLKKLAPDSAQNFYFQALDAYTRGEKL
                     KALALIKKSISKDPLLTPAYTIGGLIYADGGKDQSALPFFERAVQQSPYDPIYLLNLA
                     LCKNRLGMKADALSLLDQALRARANLAEAMYWKGVLLAENGQNAEAQKYARGAVDAGL
                     KNVSAYRLWLQLAFEAADDVDVARAVSELSQDARESARWRLAAEYSFRFGDFRKAVSL
                     LDRAFQIDEGMPEDRRMYVRCLFAEGQDPMPWIRSQRLSDAERQELRTLVDLLQNTPG
                     IVQSRDPLVRPAK"
     gene            complement(2194..2904)
                     /locus_tag="PLG78_11960"
     CDS             complement(2194..2904)
                     /locus_tag="PLG78_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNE23878.1"
                     /translation="MNAFLPAAGFGTRMGDLTRSIPKPLLPVGGVPMIYYALFQLWRW
                     KVDRVVVNLHFLKEKIERELSGFKAMEIIFSREDPILGTAGGIRNALPLFSSDAIVVL
                     NPDSIVFTDRSLEQITSAASSSWDSLLGIAPLPQGSANTPLSWKEGQLSFEESGSYYY
                     MGASVLKLSQFDHVASGYSELGSLWREQAYEGRLEGFLYEGNLLDAGNASEYRSLPEQ
                     IVPGHLAASWKEFIKNCK"
     gene            complement(2904..3905)
                     /locus_tag="PLG78_11965"
     CDS             complement(2904..3905)
                     /locus_tag="PLG78_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE23879.1"
                     /translation="MKPLLGDVREWLKELDLPEPTNILPLQKDLSTRTYYRLTLNEVC
                     EWGDSLVLCVHSGQYEATVSDFVSISRQFKEAGVAAPDVIAQKQHWLLQTDAGSIDLR
                     QHCLEWIEKGDTQSIEITYERLLDQLVRLQAVTPSHPVSGRFFDFDKLHFEMEHFALH
                     VAQAGQKFAREYFYSFEFQMFLKTICEKLAQSSSKVFVHRDLHSKNVMRSSENLVWID
                     FQDARSGLRYYDLASLLYDPYLPLSRDFRTRMLSSYAKKSGKAVEENLFYMQSFQRLV
                     KAIGTYMSVIHTRQDRDYLGFLRQAAIHLEEVVQLGGFPDHTFLFVRGMQKFSELEF"
     gene            complement(3902..>4887)
                     /locus_tag="PLG78_11970"
     CDS             complement(3902..>4887)
                     /locus_tag="PLG78_11970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002751150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE23880.1"
                     /translation="KFKRENGLSYEPKEVIVSTGGKQVLFNLFLAYLNPGEEVIVPAP
                     YWVSYADIVRIVGGVPVIVQCPASQNYLIQPSQIEEAVTPRTRAILLCSPSNPTGSVY
                     SEAELRAIAAVLEKYPDILIISDDIYEHILFDNRKFTNLAMLFPEWKDRIFIANGVSK
                     SYSMTGWRIGYGAGNRALIEGMETMQSQSTSNPSSISQAAALAALEGPQECVSTMRVA
                     FEKRKNIVMQKFSQVPEITLSAPGGAFYVFPNLDRVFALPGFQKLRAASKDRSLSRIL
                     SAHLLEKYDVAVVPGIAFGDDSGMRLSFALGEDALIKGLDRIAKMVADLRS"
BASE COUNT         1267 a         1253 c         1212 g         1155 t
ORIGIN      
        1 gcgaacaatt ttgttaggcg cggagcgtgg cgcgtagatc ttttgcaatt gtgaaagcgc
       61 gcttagttct ggtctgcttt gttgcaacct gcgctctctg gtatcttctt ccgaaccccg
      121 gatccgcgga agagactgca ataccatctt accccgccgc gaaccctggc aaagcgacga
      181 tcctttttgg aggagataca cactttcaat ggggcatcga ggaactgatt gaaaaggagg
      241 atgctctcgc gcctgtgcgc gaaatgcaac gcactttcga aaaggcagat tttcggattc
      301 tgaatgtgga aaccgctatc acggacaaag gagaacctgc gtcaggtcgg tcccacatat
      361 ttcgcgcaag tccgggttcg atcaaaacac tgactccgct caagctgaat ctggccatac
      421 ttgccaacaa tcatgccatg gatatggggc ccgaaggatt ggaagatacg agacagaacc
      481 tgcgaaaggc aggaattctg acggcggggg tgggagcaaa tgcaaaggaa gcgtctcttc
      541 cagtgctctt tcgaatcggg gacattcgat tcgccgtagt tgccgcatca gccatcggcg
      601 atgagcgtat gtgggcagac gacaaaacac ccggcattgc aaagccggag caaattctag
      661 cgcaaattca agctgctcga actctggcgg accacgtcat tgtaagtctc cactggggaa
      721 tggagtacaa tccatatcca atgccggacc agcgcccact ggctcaacaa ttcatccagg
      781 cgggggcgtc tgctgtgatt gggcaccatc ctcacgtcgc acaggggatt gagacttcca
      841 aaaataccgt tatctgctat tcactgggaa attttttgtt tggaagtagc aatccattcc
      901 aaacagacaa catgattgta ctcatggaat ttgaagctaa aaagcctgga gtgcggggac
      961 tccgtgtgat tccaatcacg ggccactata gagaaagcgg ccatgcgcca cgccctcttc
     1021 acacaacaga actccattcc ttttttccgg atctatggac tcagtctaca aagatcaatc
     1081 caagcatgtc ttctcgcatt atagaggaag aggatgggat caggatcaat gtcgactaac
     1141 gtggcaacaa ccgcgataat tatttagcgg gacgaaccag gggatctcta ctctgaacta
     1201 taccgggagt attctgaagt aaatcaacga gcgtccgcag ttcctgacgt tctgcatcgc
     1261 tcagccgctg agatcgaatc cagggcattg gatcctgacc ttctgcaaat agacatctaa
     1321 catacatgcg gcgatcctcc ggcattcctt cgtctatctg gaaggctcga tccagcaagg
     1381 aaaccgcttt gcgaaaatcc ccgaatcgaa acgaatattc ggctgcgagt ctccagcgtg
     1441 ctgattctcg cgcgtcctgc gaaagctccg acactgcgcg cgccacgtct acatcgtccg
     1501 ccgcctcaaa cgcaagctgc agccacaggc gatacgcaga aacattcttc aaaccggcat
     1561 caacagcacc gcgtgcatat ttttgcgctt ccgcgttctg accgttttcc gcgagaagga
     1621 cacctttcca atacatggcc tccgctagat tcgcgcgagc acgcaaagcc tggtctagca
     1681 acgagagagc atcggctttc attccaagac gattcttgca gagcgccaga ttgagcaggt
     1741 agatcggatc gtaaggactt tgttggaccg cacgctcgaa aaacgggaga gccgattgat
     1801 ccttgcctcc gtctgcatag atgagtccac ctatggtata ggccggggtc aagaggggat
     1861 cctttgaaat cgatttcttg atgagagcca gagccttcag tttctcaccg cgagtgtacg
     1921 cgtccagagc ctggaaatag aagttttgcg cggagtccgg cgcgagcttt ttcaggtctt
     1981 ccagtaaacc aggaatttca aacttctttt gcagtctaaa ggcagcatcc agggacgcag
     2041 tcaatctctt gagttctaag tccgtttctg cacgggtgcg agcttccttt gagggtcgcg
     2101 agtccggttt ggcgggagta gttggcgttg ccggagaaac attttgaatg ggcgtttccg
     2161 caaacaacgg cactgcaaac acaaggagca gaattatttg caatttttta tgaattcttt
     2221 ccatgatgcg gccagatgcc ctgggacaat ttgctctgga agggaacgat attcagatgc
     2281 gttgccggcg tccaacaggt ttccttcata caggaaaccc tccagtctgc cttcgtacgc
     2341 ctgctctctc cacagagaac ccagctcact gtaaccggag gcaacatgat caaattgtga
     2401 cagcttgagc acggacgcac ccatgtagta gtaggaaccg ctttcctcga aactaagctg
     2461 accttccttc caggacagag gagtgttggc tgaaccctga ggcaggggag caattccaag
     2521 caagctatcc cacgacgagg acgcagcaga cgtgatttgc tcgagacttc gatcggtaaa
     2581 cacaatgctg tccggattca aaaccacgat cgcgtcagac gaaaacagtg gtagagcatt
     2641 gcggatcccg cccgcggtac caaggatggg atcctcacgc gaaaaaataa tttccatggc
     2701 cttgaatcct gagagttcac gctctatctt ttcctttaaa aaatgcaaat tcacaacgac
     2761 gcggtccact ttccatctcc agagttggaa cagagcgtag taaatcatcg ggacaccgcc
     2821 cacaggcaag aggggtttcg gaatggaacg cgtcaggtct cccatgcgag tgccaaaacc
     2881 ggctgctggc aagaatgcgt tcatcagaat tctaactctg aaaacttctg cattccgcgg
     2941 acgaacaaaa atgtatggtc cggaaatccg ccgagctgaa ccacttcttc cagatgaatc
     3001 gccgcctgac gtagaaatcc aagatagtcc ctgtcctggc gcgtgtgaat cacggacatg
     3061 taagtgccga tcgcctttac caggcgctga aatgattgca tgtaaaacag gttttcctcc
     3121 acggctttcc cggacttttt tgcatagctg gacaacatcc tggtccggaa gtcacgagac
     3181 agaggcaggt agggatcgta gagcaaactt gccagatcat agtatctcag tccgcttcgc
     3241 gcgtcctgaa aatcaatcca taccaggttt tcagaagagc gcataacgtt cttgctgtgg
     3301 agatctcgat ggacgaaaac cttggatgat gattgcgcaa gcttctcaca tatagtcttt
     3361 agaaacattt gaaattcaaa actgtaaaag tactcgcgtg caaatttctg ccccgcctgt
     3421 gcgacgtgga gagcaaaatg ttccatctca aaatgcaatt tatcaaaatc aaagaaccga
     3481 ccagataccg ggtgagacgg agtcaccgcc tgcaaccgca caagttgatc cagcagacgc
     3541 tcatatgtta tttcaatcga ctgcgtatct cccttttcga tccattccag acaatgctga
     3601 cgcaaatcta tggaaccggc atctgtttgg aggagccagt gctgcttttg cgcgatcacg
     3661 tcaggcgcgg ccacaccggc ttctttgaac tgtctgctaa tgcttacaaa atcagaaaca
     3721 gtggcttcgt attgaccgga atggacgcac aaaacaagag aatcacccca ttcacatact
     3781 tcatttagag tcagcctgta gtatgtgcga gtggagagat ccttctgcaa aggtagaatg
     3841 ttcgtgggct caggaagatc aagttctttg agccactcac gaacatcacc taacagaggt
     3901 ttcatgatcg caagtcggct accattttgg caattcggtc caggcccttt atgagagcat
     3961 cctctccgag tgcaaagctc aatctcattc cgctatcgtc accaaaggca attcccggga
     4021 ccactgccac gtcatatttt tcaagcagat gcgcagacaa aatgcgcgaa agagatctgt
     4081 ctttcgatgc tgcgcgaagt ttttgaaatc caggcagcgc aaatacgcga tccaggtttg
     4141 gaaacacgta gaaggcacct cctggtgcag acagagtgat ttcgggaact tgcgaaaact
     4201 tctgcatcac aatatttttt cttttttcga aggccacgcg catggtggag acgcactcct
     4261 gtggcccttc gagtgctgcg agtgcggctg cctgagatat ggagctcggg ttcgatgtgc
     4321 tctgagattg catcgtctcc atgccttcaa tgagtgcacg gtttccagca ccgtagccaa
     4381 ttcgccatcc ggtcatactg tatgacttag aaactccatt ggctatgaag atgcgatctt
     4441 tccactcagg aaatagcatg gcgagatttg taaactttct gttatcaaac aatatgtgtt
     4501 cgtatatatc atctgaaatg ataagaatgt ccggatactt ttctagaaca gcggcgatcg
     4561 cgcgaagctc ggcttctgaa tacacgcttc cggtgggatt cgaaggagag caaagaagga
     4621 tcgcacgagt gcgcggagtg accgcctctt caatctgcga aggttgaatc aaatagttct
     4681 gcgatgcagg acactgaacg atgactggaa ctccgccaac aattctgact atgtcagcat
     4741 acgagaccca gtacggcgcg ggtacaatca cttcttcgcc cggattcaga taggcgagga
     4801 atagattgaa cagtacttgc tttcctccgg tagaaacgat gacttctttg ggttcgtaac
     4861 tgagaccgtt ctctcttttg aatttgt
//
LOCUS       DAOQJL010000273         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10227_length_4883_cov_3.530033, whole genome shotgun sequence.
ACCESSION   DAOQJL010000273 DAOQJL010000000
VERSION     DAOQJL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10227_length_4883_cov_3.530033"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLG78_11975"
     CDS             <1..616
                     /locus_tag="PLG78_11975"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE23881.1"
                     /translation="VLQEAGIPNFIAWFIAGRLPSFGFFYKLFVVPFSALWNLIRRIF
                     TKRARSQINPGNYIASLMDQDSYLRKTMVLLGMGRDRSDGEVTLNDDDEPIIRWRMDH
                     SRLHYDRVRREMEKMAHALGGKFMENPISYMDKIIAVHPLGGCVMADTAEDGVVDTRG
                     EVFGHPGLFVVDASVLPTSVGPNPSLTITALAEYFASKWPKKGA"
     gene            660..2507
                     /locus_tag="PLG78_11980"
     CDS             660..2507
                     /locus_tag="PLG78_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23882.1"
                     /translation="MFAACAPAGEGVKHGTVSLSPSTITSLRGTWTLCLSDSDCREFP
                     VPGGWIPHGLRITSATYRLNVKSTPGMKIGLVIPRTLAAYEVYWNHSLAGKSGKMEDA
                     ASSYEALTKRLFLFPESAAGEDLLEIRVAGIGEVGGVVQPEIYAGPAGPVSDLYFARR
                     TVSSILAGVFLFAGFSHLLFYFSRPKQFPYLYYSIACLLSTVAMIAAEGITYLITPSG
                     VFNTISLTTVLALMPPATLLFVEGFFGRSQSAITYVVIAAGLALCAVLWTGLFSIQAF
                     VFVVRYFLPVLMVFMLSSYFYIVYLVIRALISGQNGAVIVLSSLLLFILGFLNDFAVN
                     RGYFRSFSLSGEGYLCFITGMSIALARQFALFQREHDQLHRQLESSENNYRSLVEGSG
                     QMIFALDLEMRVTSANQACRSLLGMDPRKLIGKPFSQLVHASGHLESWLAGISTLMET
                     RKPVRFSAVLATSLGEPRAMDLTLEYANSGLGDFILGKASGRHADEIQAMCEEETQYY
                     VMSNYIVLAELLSRRLTAGIEKTAGAEAALAVEIGLRELLVNAVEHGNLEISFEEKSG
                     LQREGKYYEALRQKQLDPVLGARKVHIQYEFKGNEFKAVIRDEGKGLSK"
     gene            2681..4084
                     /locus_tag="PLG78_11985"
     CDS             2681..4084
                     /locus_tag="PLG78_11985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23883.1"
                     /translation="MKRPRIQLAISLRALTLILTTAFAAVTLVIAYGSARVITRNRVK
                     EANVSRAAERLNYLHFASESYLRLGQENRLILLRSALQELMSSFAGSPDLNVVMVTAP
                     DGTIIASSHPEEAGRTWESTNYGINAKIAEGVIRNRLARVIVDEDRSVVEGYTFMCDR
                     SAVSHPEPGTCGFLFYQLNLAYHMEAAGSDLVAQTVITGAGLLGGSGVLWWMLTYLIT
                     RRAERIGRGLRSFAEGQRNHRIDLGGQDEIARLAQSVDRLFDRVESDEAKIHDREERL
                     IHINEQLKKANGLLAEAAVTDSLTGLFNRRYFDARIEEEIRRAQRQGLAVSLIMCDVD
                     HFKPYNDHYGHQAGDQCLQKVASEIHTCFRRGGEFSARYGGEEFVIILPGLDLDQAWT
                     RAEDLRTIVEKKRIPHDASPTAPYVTLSLGVACHHPNDAGEPQISAIKLLRRADETLY
                     RAKESGRNRTEREVASN"
     gene            complement(4187..>4883)
                     /locus_tag="PLG78_11990"
     CDS             complement(4187..>4883)
                     /locus_tag="PLG78_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE23884.1"
                     /translation="AVDYLSTLKKVRVVLVAGAGKSFTTGLDLEEFFEQFKETLHGAT
                     GEEREKLAELIGVMQSGFRRMMSSPQVYIGCVHKHCIGGGLDLICACDVRLASKDASV
                     SLRETKVAIVADMGSLNRLPFIVGDGHTRLMAFTGRDFSGEECKAMGLFSEVYESQDK
                     MMTAARDLAREIASNPSIILRGVKQNLNFQNGKSTLEGMDYVTTYNAAFLDTAALREM
                     MTAFKERRRPVFD"
BASE COUNT         1109 a         1189 c         1302 g         1283 t
ORIGIN      
        1 tgtattgcag gaagcgggaa ttccaaactt catcgcctgg ttcatagcag gcagattgcc
       61 ttctttcggg tttttctaca aattgtttgt ggttcctttc tctgcgctct ggaatctcat
      121 ccgcagaatc ttcacgaaac gtgcgcggag tcagatcaat ccaggcaact acatcgcatc
      181 tctcatggac caggatagct atttacgaaa aacaatggtt cttctgggaa tgggtcgtga
      241 tcgttctgat ggggaagtga cgttaaacga cgatgatgaa ccgatcattc gctggcggat
      301 ggatcatagc aggcttcact acgatcgcgt cagacgcgaa atggaaaaaa tggcccacgc
      361 cctcggggga aaatttatgg aaaatccaat ctcttacatg gataagatca ttgcggttca
      421 tcctctggga ggatgcgtga tggcagacac ggcggaagat ggagtcgtgg atacgcgcgg
      481 agaagtgttc gggcatccag gtctctttgt cgtggatgca agtgtactgc ccacttccgt
      541 tggccccaat ccttccttaa ccatcactgc tctggcagag tacttcgcaa gcaagtggcc
      601 gaagaaaggg gcgtgatttg cgactggcgc gagcggtttt tgtattgcct ctgttcctga
      661 tgtttgccgc gtgtgcgcct gcaggagagg gagtaaaaca cggcactgtg tcgctttcgc
      721 cgtctaccat tacatctttg cgaggaacct ggactctgtg tctttcggat tcagattgca
      781 gggagtttcc cgttcctggc ggttggattc cccatggtct tcgcataacc agtgctacat
      841 acaggctcaa tgtgaagtca acgccgggaa tgaagatcgg cctcgtgata ccgcgaacgc
      901 tcgctgctta tgaggtgtac tggaaccatt ctcttgcggg caaatccggg aaaatggagg
      961 atgcggcctc ttcctatgaa gcgctcacca aacggttgtt cctttttccg gaatctgcgg
     1021 ccggcgagga tctactggaa atccgcgtgg ccggaattgg agaagtgggc ggagtggtgc
     1081 agcctgagat ttatgccggg cccgcggggc ctgtgtcaga tctgtatttc gcgcgacgga
     1141 ctgtctcttc gattcttgcc ggtgtgtttt tgtttgcagg cttcagtcac ttattgttct
     1201 acttcagtcg tccaaaacag ttcccatatc tatactattc gattgcctgt cttctttcca
     1261 ccgtggccat gattgcggcg gaagggatca cgtatctcat cactccgtct ggcgttttca
     1321 atacgatctc gcttacaaca gtgcttgcat tgatgccgcc cgctaccttg ctcttcgtag
     1381 aaggattctt tggacgttcg cagagtgcca tcacttatgt tgtgatcgcc gcgggccttg
     1441 cactgtgcgc ggttctgtgg accgggctgt tttctattca ggcttttgtg tttgtggttc
     1501 gttactttct gccagttctg atggtcttta tgctctcgtc ctatttttat atagtttatt
     1561 tagtaattcg agctctcatt tccggacaga atggggccgt gattgttctt tcgagtttgc
     1621 ttctttttat tcttggtttt ttgaatgact ttgctgtgaa cagaggttac tttcgcagct
     1681 tttctctctc gggagaagga tatctgtgct tcattactgg catgtctatc gcgctggccc
     1741 gtcagtttgc gctcttccag cgggagcacg accaactcca caggcaactg gaaagttctg
     1801 agaacaatta tcgcagtctc gtagagggat cgggtcaaat gatcttcgcc ctggatctgg
     1861 agatgcgcgt cacatctgcc aatcaggcat gcagatctct tctcgggatg gatccccgca
     1921 agctcattgg aaagccgttc tctcagctgg ttcatgcgtc cggacatctg gaaagctggc
     1981 ttgcggggat ttcaactctc atggagacta gaaagccagt tcgcttttcc gcagttcttg
     2041 ccaccagtct gggtgaaccg cgtgccatgg atttgactct ggaatatgcg aattctggcc
     2101 tcggggattt cattctggga aaagcgtctg gccgccatgc agatgaaatc caggcaatgt
     2161 gcgaagagga aactcagtac tacgtaatga gtaactacat tgtcctggca gagctacttt
     2221 cgcgcaggct aactgctggc atagagaaga cggctggtgc agaagcggca ctcgccgtgg
     2281 aaattggtct tcgtgaactc ctggtcaatg cagtggagca cgggaatctg gagatcagct
     2341 tcgaagaaaa gagcgggctt cagcgcgaag gtaaatacta cgaagcactc aggcaaaagc
     2401 aactcgatcc cgttctggga gcgcgcaagg tgcacatcca atacgagttc aaaggcaatg
     2461 agttcaaagc agtgattcga gatgaaggaa aaggcttatc aaagtagccg agcttggaca
     2521 attgcagcat ggtcggggac ttcttctggc aagggccgaa ttcgacagcg tgacttataa
     2581 cgacgcgggc aacgcggtga ccgttacgaa gaagttcgct tccgctccgg aatcaggctt
     2641 gcattgacag cagcgcgact ttatttacaa ccgtcgtcag atgaagcgcc cgcgcattca
     2701 acttgcaatt tctctgcgag cgctcacgct catccttaca acagccttcg cggccgtaac
     2761 gctggtcatt gcgtacggaa gtgctcgagt gattacacgc aatcgtgtga aagaagcgaa
     2821 tgtttcacgg gcggctgagc gtcttaatta ccttcacttt gccagtgaaa gctaccttcg
     2881 cctcgggcaa gaaaatcgtc tgatccttct tcgcagtgct ttgcaagagt tgatgtccag
     2941 cttcgcgggc tcgccggatc taaatgtagt tatggtaaca gctccggacg gaaccatcat
     3001 cgcgtcgagc catcccgaag aagcaggtag aacctgggaa tccacaaact acggaatcaa
     3061 cgcgaaaatc gcagaaggtg tgattcgaaa tcgtctcgca cgcgtgatcg tggatgaaga
     3121 tcggtctgtg gtagaaggtt acacattcat gtgcgatcgt tccgctgttt cgcatccaga
     3181 gccaggcact tgcggctttc tgttttatca gttgaacctt gcctatcata tggaagctgc
     3241 tggatcagat ctggtggcac aaactgtcat tactggagcg ggactgctgg gtggatcggg
     3301 tgtgctctgg tggatgctta cttatctcat cacgcggcgt gcagaaagaa ttggaagagg
     3361 gctgcggagc ttcgcggaag ggcagagaaa tcatagaatc gatctgggcg gtcaggatga
     3421 gatcgcgcgc ctggcgcaat ccgtggatcg actgtttgac cgggtggagt cagatgaagc
     3481 aaaaattcat gaccgcgaag aaagactcat tcacatcaat gaacaattga agaaagcaaa
     3541 tggactgctt gcggaagccg cggtgacgga ttcgctcaca ggcctgttca atcgcagata
     3601 ctttgacgca cgcattgaag aagagattcg tcgcgcgcag agacaggggc tggcggtgtc
     3661 tttgatcatg tgcgacgtgg atcatttcaa gccatacaat gatcactatg gtcaccaggc
     3721 cggagatcaa tgcttgcaaa aggtggccag cgagatccac acgtgctttc gccgtggtgg
     3781 agaattctcc gccagatatg gcggagaaga attcgtcatt attcttcctg gattggacct
     3841 ggatcaggcc tggactcgtg cggaagatct aagaactatc gtggagaaaa aaagaattcc
     3901 acacgatgca tcgcctacgg ctccgtatgt gactctcagc ctgggagttg cctgtcatca
     3961 tccaaatgat gcaggcgaac ctcagatttc tgcgattaag cttttgcgcc gggcagatga
     4021 aactctctat cgtgccaaag agtcaggtag aaatcgtacg gagcgcgaag tagcgagcaa
     4081 ctaacctttg ttctacgaaa cgtgatgcgg ccagttgcca acgaggatcg tcgagatcca
     4141 gtacacaatc ttgatgactt tgtctttcat gcgaactccc gtgtgattag tcgaatacgg
     4201 gtcttcttct ttctttgaac gccgtcatca tttcacggag cgcggctgtg tccaggaatg
     4261 ctgcattgta tgtggtcacg taatccattc cttccagtgt ggatttgcca ttctggaaat
     4321 tcaggttctg ttttactcca cggagaatga tagatggatt ggatgctatt tctcgcgcca
     4381 ggtctctggc tgccgtcatc attttgtcct ggctttcata cacttcggaa aacaatccca
     4441 ttgctttgca ttcttctccg ctgaaatcac gtcctgtaaa agccatcaaa cgcgtgtgac
     4501 cgtctcctac aatgaatggg agacgattga ggctacccat atccgctacg atggccactt
     4561 ttgtttcgcg caatgaaaca gaagcgtctt tcgaggcgag tcttacgtcg catgcgcaaa
     4621 tgagatcgag tccgccgcca atgcaatgtt tgtgcacgca accaatgtat acctgcgggc
     4681 tgctcatcat tctacgaaat ccggactgca tcactccaat caattctgcc agtttttcgc
     4741 gctcttctcc agtggcaccg tgaagcgttt ctttgaattg ctcaaagaat tcttcgaggt
     4801 ccagtccagt agtgaatgat tttcccgcgc cggccacaag aaccacgcgc acttttttta
     4861 gagtgctcag ataatccact gcc
//
LOCUS       DAOQJL010000274         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10261_length_4870_cov_4.014953, whole genome shotgun sequence.
ACCESSION   DAOQJL010000274 DAOQJL010000000
VERSION     DAOQJL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10261_length_4870_cov_4.014953"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLG78_11995"
     CDS             complement(<1..1314)
                     /locus_tag="PLG78_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23885.1"
                     /translation="MVHLRLLSSGGKKRVLRWIFASLFLLLVLAYNIAFYPPVFEKIL
                     IRGLTGVIPGRITAHVEKASLFRGFVFKDVEVRSENSPFFKASHVTLSYHLPSLLIGH
                     IGIRDFSLVRPEIFLIKKNGVWNTDEVFGPPANKEPEPETPPRKSISTYVAVKLYGKF
                     VIQDLHFNMDVQGKEAQFLDVDHLDLHAAFITRTFQEVPLNLHAFELFDTLMIGLAPN
                     RPVNVVHRGAGDVSGEVKLPFFIFRDVSRGEPEFNSRFHLNTGNLTLRKNGRSLQPQV
                     RLYYNVVYDAKKDSLRLGRLLAEQSGSEWMDLSANVDRLTSKNPSITLDMKQSHMDLT
                     SVETILNFLGTTMNLHGNLSLFPLHMEGPLQSVSMNGSLRGNAVSLKTGNLYHSIPDL
                     HLDMSAKMDLLQYMPVDVPEEKKERSGLAFGVFHSLLVPDLFVSYN"
     gene            1402..2142
                     /locus_tag="PLG78_12000"
     CDS             1402..2142
                     /locus_tag="PLG78_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE23886.1"
                     /translation="MDELFSFQDRVILITGAGRGIGKAFAEAFHKRGAIVYGTGSRKE
                     SADHMNAFGIQGRVADATDPDAMPGLIDEIMQTHGKLDCLINNSGISSDTPASFFKED
                     EMEKILNTNVKGVFRACQAYFKAHRKKGGVIVNVASVLGLYGFPLASIYSASKGGVIQ
                     LTRALAIEWASSGFRLNALCPGFIETDMTARMTGKAEYKSKAEEGIPMKRIGKPEDLV
                     GAALFLASPASSYMTGQVLIVDGGLTAQ"
     gene            complement(2174..3019)
                     /locus_tag="PLG78_12005"
     CDS             complement(2174..3019)
                     /locus_tag="PLG78_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23887.1"
                     /translation="MQKGINEILSQFKQDDYTVKLCSSLFSVVPFAPPFQFYNSLEGA
                     AGRMQATSDTVTKANALAASEDVKNAVWVADALDTSDKLIAGYAGVKNLLSLFSSSSA
                     PKARTFESDPQQAVDAGLKALGLAYMIQKLFAGSITEKVNAFRELPAGQEIGLYYATA
                     EIALPFADNLVEGGASVVERLVGKHKGDIQAKFAQFAGQEQVANAMGMLENLIKPLDE
                     YVNKAKQYAGPVTDKIKHYLPSAMNVADSATGMAAAGVDLMPVWSFLGGRLAAEACLL
                     RARNG"
     gene            complement(3135..4430)
                     /locus_tag="PLG78_12010"
     CDS             complement(3135..4430)
                     /locus_tag="PLG78_12010"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNE23888.1"
                     /translation="MSETAKLIFRDKQLELPVLTGTEDEIAVDIGKLRGSTGLITMDD
                     GYMNTGSCTSAVTFLDGEKGILRYRGIPIDQLAEKSTFLEVAYLLIYGELPSQKQYEA
                     FSTQVTRHTLIHEDLKRLYDGFPKDSHPMAILSSMICTLSGYYTDSSDPLNPRHREVS
                     IMRLLAKTPTIAAYSHKKSIGQPFVYPRNALSYVGNFLNMMFSVPAEDYEVDPIVEKA
                     LDLLLILHADHEQNCSTSTVRLVGSSRANIYATIASGVCALWGPLHGGANQAVIEMLE
                     LIRDDGGNVAKFVELAKDKNSTFRLMGFGHRVYKNFDPRARIIKKACDDVLNKLGVKD
                     PLLDIAKKLEEAALTDPYFVERKLYPNVDFYSGIIYRAIGIPTEMFTVMFALGRMPGW
                     IAQWKEMIEAPGTKIGRPRQVYTGYAERDYVPREKRPVD"
     gene            4526..>4870
                     /locus_tag="PLG78_12015"
     CDS             4526..>4870
                     /locus_tag="PLG78_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE23889.1"
                     /translation="MRFVFLLAALCVFAGCSSLPSGAEPVREIHNQVYAEHEGKNLMA
                     DVFLPQSPGPHPAILLLHSGSWRHGSKSRMADVGRAFAREGFVAVAANYRLTNEARFP
                     AQIHDTRDALRWM"
BASE COUNT         1211 a         1231 c         1230 g         1198 t
ORIGIN      
        1 gttataagac acaaacaaat ccggcacaag aaggctatgg aacaccccga acgcgaggcc
       61 ggatctttct ttcttctcct ccggaacgtc caccggcata tactggagaa ggtccatttt
      121 cgcagacatg tccaggtgaa gatcaggaat actgtgatat aaatttcccg tcttcagtga
      181 cacagcattt ccacgcaagg atccgttcat gctgacactt tgcagcgggc cttccatgtg
      241 aagaggaaac agagaaagat tcccgtgcaa gttcattgtg gttccgagga aattgagaat
      301 cgtttccacg gaggtaagat ccatgtgaga ttgcttcatg tccagggtga tagatggatt
      361 cttagacgtt aggcgatcta catttgcgga aagatccatc cactcagaac cactctgctc
      421 tgcgaggaga cggccaagac gaagactgtc ctttttcgca tcgtatacta cattgtagta
      481 caatcgaact tgaggttgaa gcgaacgacc attcttgcgc aaagtgagat tgcccgtgtt
      541 gagatgaaac cgcgaattga actctggctc cccgcgggac acatccctga aaataaaaaa
      601 tggaagcttc acttcacccg atacgtctcc tgccccgcga tgtaccacat ttactgggcg
      661 attgggagcc agaccaatca tcagagtatc aaacaattcg aaagcgtgga gattgagcgg
      721 cacttcctga aacgttcgcg tgataaaggc cgcgtgtaaa tccagatgat ccacgtccag
      781 gaactgcgct tcctttccct ggacgtccat attgaaatgc agatcctgaa ttacgaattt
      841 tccgtacaat ttcacagcca cataagtgga gatggacttt cgcggtggag tttctggttc
      901 cggttctttg tttgcgggag ggccaaacac ttcatctgta ttccaaacgc cgttcttctt
      961 gatgaggaag atttccggac gaaccaggct gaagtctctg attccaatat gcccgatcaa
     1021 cagagaaggc agatgatacg aaagagtaac atgactggct ttgaaaaatg gagaattctc
     1081 tgaacgcact tctacgtctt tgaatacgaa tccacgaaac agactcgcct tttctacgtg
     1141 ggctgtgatc cgaccaggga tgactccagt caaaccgcgg atcagaatct tctcgaatac
     1201 tggcggatag aacgcaatgt tataggccag gaccaggagc aaaaacaaag aggcgaaaat
     1261 ccacctgagt actctttttt taccaccgga gctcaacagg cgtaaatgga ccacgggcgc
     1321 gatccatgaa aagaaaaaaa cactcgaacc cgaatatcca aaagtgaacg acagtacagg
     1381 cacggggcgc acttaagcgc tatggatgaa ctgttttctt ttcaagaccg tgtcatattg
     1441 atcaccggag ccggccgggg gatcggaaag gcgttcgcgg aagccttcca caagcgtggt
     1501 gccattgtat atggcacagg ctcgcgcaaa gaatcggcag atcatatgaa cgcttttggg
     1561 attcagggca gagtcgcgga tgcaaccgat ccggacgcaa tgcccggact catagacgaa
     1621 atcatgcaaa cgcatggaaa gctcgactgc ctcatcaaca actctggaat ttcatcggac
     1681 actcccgcgt cctttttcaa ggaagatgaa atggagaaga tcctcaatac gaacgtgaag
     1741 ggagtctttc gcgcctgcca ggcctatttc aaagcgcaca gaaagaaggg cggagtgatt
     1801 gtgaatgtgg cttctgtact cgggctctat ggcttcccac ttgcgtccat ctattctgct
     1861 tccaagggtg gagtcatcca gctcacgcga gcgctcgcca tagaatgggc cagctcagga
     1921 tttcgcctca acgctctttg cccgggattc attgaaacgg atatgactgc gagaatgaca
     1981 ggcaaagcgg aatacaaatc caaagcggaa gaaggaattc ctatgaagcg aattggcaaa
     2041 ccggaagacc tggtgggtgc cgctttgttt ctggcatctc ctgccagttc ttacatgacg
     2101 ggtcaagttt tgattgtaga cggaggcctt acggctcaat gaggcgggca ctcgcccgcc
     2161 ttccattaaa gaatcaaccg tttcgcgcac gcagcagaca ggcttctgct gcgagtcttc
     2221 cacccaggaa actccacact ggcatcaaat cgactccggc tgcagccatg cctgttgcgg
     2281 aatcggcaac attcatagca ctcggcaggt agtgtttgat tttgtctgta acagggcccg
     2341 cgtattgctt tgccttgttc acgtattcat cgagcggctt gatcaaattt tcaagcattc
     2401 ccatggcgtt ggctacttgc tcctgacctg cgaactgagc gaattttgcc tggatgtctc
     2461 ctttgtgttt tccaaccaga cgttccacca cagacgctcc gccttctacc aggttatccg
     2521 cgaacgggag ggcgatctct gctgtagcgt agtagagacc aatctcctgg cctgcaggca
     2581 attcacgaaa tgcatttact ttttctgtaa tggaaccggc gaacaacttc tgaatcatgt
     2641 aagcaagacc cagagccttc agtcccgcat ccacagcctg ctgtggatcc gattcaaacg
     2701 tgcgcgcttt gggagcgctc gaagacgaaa acagggagag gagatttttg acccctgcgt
     2761 atcctgcgat cagcttgtcg cttgtatcga gcgcgtctgc aacccagact gcattcttca
     2821 cgtcttcgga ggcggcgagt gcgtttgcct ttgtgactgt atcgctggtt gcctgcattc
     2881 tacctgccgc accttccagt gaattgtaga actgaaacgg cggtgcgaac ggcaccacgg
     2941 aaaacaggct ggaacacagt tttactgtgt agtcatcctg tttgaactga gatagtattt
     3001 cattgattcc tttttgcatg atcactcctt ttgtaaacag ttagagaata caaaccgctc
     3061 ccgtgcagga aagcagattt tcggaggcga tttcgccgcg cattcccagg cgcggccggt
     3121 agctcttttc tagctcagtc tactggacgc ttttcgcgtg ggacgtagtc acgctctgca
     3181 tagccagtgt agacctgacg aggacgaccg atctttgttc cgggcgcctc tatcatctct
     3241 ttccactgtg caatccagcc aggcatacga ccgagtgcga acatgactgt aaacatctct
     3301 gttggaattc caatggcacg atagatgatg cccgaataga agtctacgtt tggatagagt
     3361 ttgcgctcta caaaataggg atccgtcaag gcagcctctt ccagtttctt tgcaatgtcc
     3421 agaagtggat ctttcactcc gagcttgttt agaacgtcat cgcatgcttt cttgatgatg
     3481 cgcgcacgcg gatcaaaatt cttatacacg cgatgtccaa agcccataag acggaatgta
     3541 gaattcttgt ctttggccag ttcaacgaac ttggcaacat ttccaccgtc gtcgcgaatc
     3601 aactcgagca tctcgatgac tgcctggttg gctccgccgt gaagaggacc ccaaagagca
     3661 cacacaccac tggcgatcgt tgcataaatg ttggcccggc ttgaacccac gaggcgaacg
     3721 gtagaagtag aacaattctg ttcgtggtct gcatgcagaa tgagaagcag atctagagcc
     3781 ttctctacga ttggatccac ttcgtaatct tctgccggaa cagagaacat catgttgagg
     3841 aagttgccca cataagacag agcattgcgc ggataaacaa acggctgccc gattgatttc
     3901 ttatgcgaat aagcagcgat cgttggcgtc ttggcaagca agcgcatgat ggagacttct
     3961 ctgtgacgcg gattgagtgg atcgctggag tctgtgtagt agccagacaa tgtgcagatc
     4021 atggaactca agatggccat tggatgagaa tctttaggaa aaccatcata caatcttttg
     4081 agatcttcgt ggatcagtgt atggcgtgta acctgggtgg aaaatgcctc gtattgtttc
     4141 tgactgggta gctctccgta aatcagaaga taggcgactt caaggaatgt ggatttttca
     4201 gcgagctgat caataggaat gccgcggtat ctaagaatgc ccttttctcc gtccaggaaa
     4261 gtcacagcac tcgtgcatga accagtgttc atgtagccgt catccatggt gatgaggccg
     4321 gtggacccgc gcaatttgcc gatatctact gcgatctcgt cctctgtgcc tgtgaggacc
     4381 ggaagttcaa gttgcttgtc tctgaagatc agtttcgccg tttcgctcat attgcctcgc
     4441 aaagcgtaat acgatgtcca gtcaggtcgt gacaagtata tttttcgtga atgcctgaac
     4501 gagccttctc tattattcta tgagcatgcg attcgtcttt ctgttagccg ctctttgcgt
     4561 gtttgctggc tgctcttccc tgccgtctgg cgcagagccg gttcgcgaaa tccacaatca
     4621 ggtttatgca gagcacgaag ggaagaatct gatggcggac gtatttctgc cccaatcgcc
     4681 aggccctcat ccggccatac ttctccttca cagtggctcc tggagacacg gcagcaagtc
     4741 acggatggcc gatgttggaa gagcgtttgc ccgggaggga tttgtggccg tggctgccaa
     4801 ctatcgcctg acgaacgagg cgcgcttccc cgctcaaatc catgacaccc gcgacgcact
     4861 tcgctggatg
//
LOCUS       DAOQJL010000275         4865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10280_length_4865_cov_4.678586, whole genome shotgun sequence.
ACCESSION   DAOQJL010000275 DAOQJL010000000
VERSION     DAOQJL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10280_length_4865_cov_4.678586"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLG78_12020"
     CDS             complement(<1..221)
                     /locus_tag="PLG78_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23890.1"
                     /translation="MNFKKTVFSLLLISLSLGAVEAKRFAIVMGSNYTGNSAGIPELE
                     LCESDAKLMEQTLKSNGSYDDATVLLGPMV"
     gene            complement(255..497)
                     /locus_tag="PLG78_12025"
     CDS             complement(255..497)
                     /locus_tag="PLG78_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23891.1"
                     /translation="MLEEGRVDRFLDLSESLRSQKPITTSPKTQEEAELLAACQDVLD
                     GMVPLMERALAQISRDEFETRVSGQIQEFLAKKEEP"
     gene            complement(538..969)
                     /gene="fliS"
                     /locus_tag="PLG78_12030"
     CDS             complement(538..969)
                     /gene="fliS"
                     /locus_tag="PLG78_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNE23892.1"
                     /translation="MMAAKKTHEAYKDTEIQTAGQGKLIVMLYEGAIRFINVAMSNMT
                     PRKYDVVNNNIIRAQDIVTELMLSLNMEEGGEIAQNLFNIYAYIKKRLLEGNVQKDNE
                     VLTEVVKLLEQLRDAWDQASRQSPPRVSVPAREGASFSITG"
     gene            complement(1098..2432)
                     /locus_tag="PLG78_12035"
     CDS             complement(1098..2432)
                     /locus_tag="PLG78_12035"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE23893.1"
                     /translation="MAEFRMFSRMRSLFNPASSVYFYGLAVNSSTILLIRFVDPVYGP
                     RIEWIPFIAFVSYYIFIALWLSRRLVRGARSLESWFDLVLITSFPVAIYYGYLSFVLP
                     PYSIVFWIWGTAVGMPGFGYIRRRYWIVWVLIMFAATIPGFVLGAFEIRIILSMAWVV
                     IMTFYMTRASNQLKKSRVALSVHLTEVRRNKRDLEKAHQKLKTAADRFQVELELAERL
                     QQGLLPTLGTLSSGLTLRGRYLAMQNLGGDFYDVATAGDKTVLLVADVTGHGVAASLI
                     TTMTKASFHAHKHLLDPSEILAAINSDLCEFLTGGQHFVTAVCVQFDVSARRVIHANA
                     GHPPGFHLSDGRLTTLGDDSLLLGLDRSLKWKSYTIEAKKSDRFFFYTDGLTETFSED
                     RSMYGERLESMLIKHSSLDSEAFLAALFDDLLEFRGSSPQSDDIAVVCVDMP"
     gene            complement(2455..4617)
                     /locus_tag="PLG78_12040"
     CDS             complement(2455..4617)
                     /locus_tag="PLG78_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE23894.1"
                     /translation="MAVVEVTHGVALCNAAGKNILFGCPPEVIKHLMVKGLGSPEVIV
                     LPDTPYRFDTLQNCTEFPLYYFLFVERNFMQGKKLTIVGTATHLRANRKLLRLTLLGP
                     TRKEYQDLGTSHWFDELYRESRALSVKDSSGRELAIDDFVDFIPFEKGVAHLPGGIRI
                     EHTGVDRFTVGEDKIDIAFNTPQPPPYDLRNDFITTMPAHFGVTVLGGASGFISDKPC
                     SGLILNYNSDHMLIDCVPFLEYALNARGISTTEIRSIFLTHIHDDHCNIFPLLRLSNK
                     VKLLATREIFWMAMMKLSLQTLMPIEDISEMFEFVEVKPYEVTEFYGLSIETHYTVHS
                     IPTIGATFRMKDGPMSRSIVFIGDNKAFDDIETMIDQGIVRPEKFAALKQKYTERHDI
                     LFADGGMGILHGNPRDALKSQSDRIVFMHLEKLPPEFDATFSHAVAGKRYSIIEGNYN
                     SYMIHTLHILGDAFRNISHEWSTALMNNFRIVTFNAGDVIFKQNEASKGLIYVILSGS
                     CSVMVHDGFTLSERTRKEAGDFVGEMAVLDENKVRSASIVAATPVTLCALDEKLFHEF
                     LIDEDRIDEMRNLWKVRSEIERFWPFEVFADNVNDRIARNSVRTRVAAGDKIVEQGTN
                     EGEFFIILSGEFSVRRNGTEVKILRAGEMFGEYGSLGDSVRNATVTALRDSVVLEIAR
                     GEIRKIVESAPIFNFSMREIMIRRSRELKKLEKSGLTT"
BASE COUNT         1279 a         1243 c         1170 g         1173 t
ORIGIN      
        1 accattggtc caagtagaac cgttgcgtca tcataggagc cgttcgattt cagagtctgc
       61 tccatgagct tcgcatccga ctcgcatagt tcgagttccg gaataccggc agagttgccg
      121 gtgtaatttg agcccatcac aatggcgaat cgcttcgcct cgacggcacc cagggaaagt
      181 gaaatgagta agagagaaaa aactgtcttt ttgaagttca tgttacccct gcataaatgg
      241 acgatgccag tgtttcaggg ttcttccttt tttgcgagaa attcctggat ctggccggaa
      301 acgcgggttt cgaactcgtc tcggctgatc tgggccaggg cccgttccat gagcggaacc
      361 atgccatcca gaacgtcctg gcaggccgcc aataattcgg cctcctcctg ggttttagga
      421 gatgtagtga taggtttctg agaacggagg gattccgaca ggtccagaaa tcgatcgacc
      481 cgtccctcct ccagcatcct tcgctgcctg cgaaggcgga agaggaggaa gcgaagatca
      541 gccagtaatg gagaagctgg ctccttcacg tgcaggcacc gatactcgcg gaggggactg
      601 tctggaagcc tggtcccagg cgtcgcgcag ctgctcgagg agtttgacca cctctgtgag
      661 cacttcgttg tccttctgca cattgccctc aagaagtctc ttcttgatat aagcataaat
      721 attgaagaga ttctgagcga tttccccgcc ctcttccata ttcaaagaaa gcatcagttc
      781 ggttacaatg tcctgcgctc gaatgatgtt gttgttcacc acatcgtact ttcgcggagt
      841 catgttagac atcgctacgt tgatgaatcg gattgctcct tcatagagca tgacgatgag
      901 tttcccctgc cctgcagtct ggatctcagt gtctttgtac gcttcgtggg tcttttttgc
      961 agccatcata acctgaaaca agtatcggtt gaatcgaaaa aaccttgaaa ccgggaaacg
     1021 ttattccacg tagaatttga tcccctccgg gtagcccgaa ggcactagaa tgcaggcgga
     1081 tgttgcagat cagcacatca gggcatatcc acgcatacga cggcaatgtc atcgctttgc
     1141 ggactggacc cacgaaactc tagcaaatca tcaaaaagcg cggccagaaa agcttcggaa
     1201 tccagagaac tgtgtttgat gagcatagat tccagccttt ctccatacat ggatcgatcc
     1261 tcagaaaaag tctccgtcaa accgtcggtg tagaaaaaga atcgatccga tttcttagcc
     1321 tcaattgtgt acgatttcca tttcaaagat cgatccagac ccagaagcag actatcgtct
     1381 ccgagagttg tgaggcgccc gtcagaaaga tgaaacccgg gcggatggcc agcattcgca
     1441 tggatcacac gccgcgcaga aacatcaaac tgaacgcata cagcagtgac gaaatgttgc
     1501 ccgcctgtta gaaattcgca caaatctgaa ttgatagcgg ccaaaatctc agacgggtca
     1561 agcaaatgtt tatgcgcatg aaaagaagct tttgtcattg tagtgatcaa ggatgcggcc
     1621 acgccatggc ccgtcacatc cgcaacgagg agcacagtct tgtctcccgc tgtagctaca
     1681 tcataaaaat ctccacctag attctgcatg gccagataac gtccgcgcaa ggttaagccg
     1741 cttgaaagcg tcccaagcgt gggcaataat ccttgctgca aacgctctgc cagctcgagt
     1801 tcgacctgaa agcggtccgc ggctgttttc aatttttgat gagccttctc caggtctcgt
     1861 ttgttccgcc taacttccgt caggtgaacg gaaagcgcca ccctgctctt cttcaattga
     1921 ttggaagcgc gtgtcatata gaaagtcatg atcacaaccc aggccatgga caagatgatt
     1981 cgaatctcaa aggcacccag cacgaatccg ggaatcgtgg ccgcaaacat aatcagcacc
     2041 cacacaatcc agtatcgtct gcgaatgtaa ccaaatcctg gcatacctac agcggttccc
     2101 cagatccaga aaacaatgga gtaaggaggc agaacaaaag acaaatagcc gtaataaatc
     2161 gcgacaggaa aactcgtgat cagaaccaga tcaaaccagg actcaaggga gcgcgctcct
     2221 cgcacgagcc ttctggacaa ccacagagca atgaagatgt aatacgaaac gaacgcaatg
     2281 aaaggaatcc actcaatgcg cggcccatac acaggatcca caaagcgaat caatagaatg
     2341 gtggatgagt ttacggcgag cccgtaaaaa tagacggatg acgctgggtt aaacagcgat
     2401 cgcatgcgac tgaacatgcg aaactccgcc atggcaaagg atcgcacgtc ccaatcaggt
     2461 tgtcaaccca cttttttcga gcttcttgag ctctcgcgat ctgcgaatca tgatttctcg
     2521 catgctgaag ttgaaaatcg gggctgactc tacaattttg cgaatctctc cgcgcgcgat
     2581 ttccagaaca acactgtctc tgagtgcagt cactgtagca tttcgcaccg aatcccccag
     2641 ggacccatat tccccgaaca tttcacctgc gcgaagaatc ttaacttctg taccgtttcg
     2701 cctaacactg aactcacccg acaaaatgat aaagaattcc ccttcatttg tgccctgctc
     2761 gacaattttg tctccggcag ccacgcgggt acgcaccgag tttcgcgcga tgcggtcatt
     2821 cacattgtct gcgaacactt cgaaaggcca gaatcgttca atttccgaac gcaccttcca
     2881 gagattgcgc atttcatcaa ttcgatcttc gtctatgagg aattcatgaa acagcttttc
     2941 gtcgagagcg cataacgtta ccggagttgc ggctacaata gaagcagatc tgactttgtt
     3001 ctcatctaga acggccatct ctcccacaaa atcgccggcc tcttttcgag tccgctctga
     3061 aagggtgaac ccatcatgaa ccatcacgct gcaggatccg gagagaatga cgtaaatcag
     3121 ccctttggac gcttcattct gtttgaagat cacgtctccc gcattgaacg tcacaatgcg
     3181 aaaattgttc atgagagctg tgctccactc atgcgaaata ttccggaatg cgtctccgag
     3241 aatatgtaga gtatggatca tataggaatt gtagtttcct tcaatgatgc tgtatctctt
     3301 tcctgccacg gcgtgagaga acgtggcgtc gaattccggc ggaagctttt ccagatgcat
     3361 gaatacaatg cgatctgatt gagacttcag cgcatcgcgt ggatttccgt gaagaattcc
     3421 catgccgccg tctgcaaaaa gaatatcgtg acgttccgtg tatttttgct tgagggctgc
     3481 gaacttctca ggtcttacaa ttccttgatc gatcatggtt tcaatgtcat cgaacgcttt
     3541 gttgtctccg ataaacacaa tgcttctaga cattggcccg tctttcatcc tgaatgttgc
     3601 gccaatcgtt ggaatggagt gcactgtgta gtgcgtctca atagagagac cgtagaattc
     3661 tgtgacctcg tatggtttga cttccacaaa ctcaaacatc tctgaaatat cttctatggg
     3721 catgagcgtc tgcagagaca gtttcatcat ggccatccag aaaatttctc gcgtggccag
     3781 aagtttcact ttgtttgaga gacgcaacag aggaaagata ttgcagtgat catcgtgaat
     3841 gtgcgttaga aaaatggatc gaatttcggt ggtggaaata ccacgagcgt tcagcgcgta
     3901 ttcgaggaac ggcacgcagt cgatcaacat atggtctgaa ttgtaattca agatcaaccc
     3961 ggaacatggc ttatcggaaa tgaatccaga agctccgcca agaacagtca cgccaaaatg
     4021 agcgggcatg gttgtgatga agtcatttcg caaatcgtat ggcggaggct gtggagtgtt
     4081 gaaggcaatg tctattttgt cttcgcctac tgtaaagcga tcaactccag tgtgctcgat
     4141 tctaatcccg ccgggaagat gcgcgacgcc tttctcgaat gggatgaaat ccacaaaatc
     4201 atcaatggca agctctcgac cggaagaatc cttcacagag agagcgcgcg attctctgta
     4261 caattcgtca aaccagtgac ttgtccccag atcctgatat tcttttcgcg tcgggccaag
     4321 aagcgtcagc cggagcagtt tgcgattcgc gcgaagatgc gtagcggtcc cgacgatggt
     4381 cagcttcttg ccctgcatga agtttctttc tacgaagaga aaatagtaga gcggaaattc
     4441 agtacagttc tgcagagtgt caaagcgata cggtgtgtct ggaaggacga tgacttccgg
     4501 actccccaga ccctttacca tcaaatgctt gattacttct ggcgggcatc caaacagaat
     4561 attttttccc gccgcattgc agagggctac accatgcgtg acttctacta cagccatgca
     4621 ggtggatcgc acaatgcacg tggacgtcca gaccttttct gcacctgaac agagtacagc
     4681 ctggcctcga tatctacaat cttagatacc cggaggagtc gggttcgcgc gagcacgctg
     4741 cgaaggcggt aagaaccgac gcatatggta gaatctcgtg gtgttgctgt aatcctcgag
     4801 cagaatgact actttcgcaa agcctctagc gtgcgccagc tgctgagccg taaaaccaga
     4861 aattt
//
LOCUS       DAOQJL010000276         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10293_length_4862_cov_4.700021, whole genome shotgun sequence.
ACCESSION   DAOQJL010000276 DAOQJL010000000
VERSION     DAOQJL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_4862_cov_4.700021"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLG78_12045"
     CDS             complement(<1..209)
                     /locus_tag="PLG78_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE23895.1"
                     /translation="MRNLILLLILFGAACGKEAERPDIEILREADSLEEARNSNGWEH
                     AGRIVPNFGFKTSGVWARFPLENKNP"
     gene            complement(206..1939)
                     /locus_tag="PLG78_12050"
     CDS             complement(206..1939)
                     /locus_tag="PLG78_12050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE23896.1"
                     /translation="MKRFRTVRFLILALAVSFALSSLFVAYAAARTVRMGRLTRESRI
                     KIAERLAFLESAGEVYFRTTNIEPLRAAMISFASETDLSVLFASDVDGKIIASTNIQD
                     VGELVANRYPRVHAKFLEDPLASGPRILPDAGTDFVVGYAGMCAPGRNGLRGGRCGFV
                     YYELDLAYRIRETNRELRTQMTVTAIGLLAGAFALWLVLTMRVTRRTETISTGIKKFA
                     SGNRSVRLRLEGADEIAELGRDVDDLFARVNETEIALKDRESRLREVFDSLLDGLIII
                     DTRGTIQSINPAGSAIFGYRDGELAGQNISVLMPEPHHSMHDVYLANYVATGVKNVIG
                     MAREAEACRKDGTKFPIDLKVSELRAEGQLLFIGIIRDISERRQMQQDLIAANQELEH
                     AVTQLAQAATTDSLTGLYNRRSLDVRIEEEIRRAQRHGQPVTLLVCDVDHFKMFNDHY
                     GHQAGDDCLIAISNILQSHFRRGGEFTARYGGEEFVVILPDLTLSEGVERAEHLRRAI
                     SSAGIPHAQSPTADHVTISIGVAVHHPDIHGEPRISARELFKNADDMLYRAKQAGRNQ
                     IQHAPVQAGGN"
     gene            complement(1936..2874)
                     /locus_tag="PLG78_12055"
     CDS             complement(1936..2874)
                     /locus_tag="PLG78_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE23897.1"
                     /translation="MLRCAAAVCAMLFFLDCEPGKSLRIGTNIWPGYAPLYLARDRKG
                     WEPGNIRLVEYPSATEVLRAFQGGALECASLTLDEALMVRESGIPFSVILIHDISQGA
                     DAIVARPLIADVKGLRGKRIGVEASALGAFVLTRALEKNKMTSADITIVPIRINEHEE
                     AFSEGRVDAVVTFEPQRSRLLKAGAREIFSSREIPGEILDVLIARRDAIEEHSDDLQN
                     VVDGWFEVNRHIQHDASLLSRVANQLKLTPGETRQAYAGIHIPDRSENIRELSGPLEE
                     HAARLGKIMAQKELLRKAPDTRGIADDSFVKRAPEK"
     gene            2976..3602
                     /locus_tag="PLG78_12060"
     CDS             2976..3602
                     /locus_tag="PLG78_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23898.1"
                     /translation="MDPVFERTCDLIDGELENWLQAVLGDILTSYDRLHLRDPLCTCL
                     LELATNANKANMKHIFFLEKGWDIYDPQLYTERLKEFRDHVLHFESTVAYAEKAKQAG
                     LYVRVSIHHSPAGLKIAVYNNLPVIPEDEKRIREKFLHALEYQNLVDFYRDYGDNSEG
                     SGLGFALMVLMLRSEGIPPSLLRVGTDGGQTAARIEVPFHPGSAPQSQ"
     gene            complement(3596..>4862)
                     /locus_tag="PLG78_12065"
     CDS             complement(3596..>4862)
                     /locus_tag="PLG78_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE23899.1"
                     /translation="TTAPWLNRWIEFSGIIALASSLSFLLVEPQTTGKIAAAMATPCL
                     LTMLIAGIRVMMSGFRPARLFTLAVVVYVIGGIVFTFRNWGYLPANNMTLYGIQFGGM
                     IQAVVFSLALADRVQVLRSNLKKHVEELETMKAAIELSEQKYRKLVEDTQDIIFSLDR
                     HFAFLTVNRAIGSHLGYSPKKIIGQRLQDITYTARSWKGELLTFQLKELETRGHTEFR
                     QAFKTSLGESRDLQVRLELLRDDGTILGKAQSPADDPLQAFFYSERTRYYVDNLISTS
                     ELASHRISRNLRRYCSEEDSQQIKMAVREMVLNAVEHGNLEIDFDTKTRAMAQGAYFD
                     LLLARQRDPAYAGRKVKVDVSLNPNRVIFQVEDEGRGFDHESAKARADQANEERLEHG
                     RGIKMTLAIFDLVQYNTRGNRVTLVKFFH"
BASE COUNT         1088 a         1254 c         1252 g         1268 t
ORIGIN      
        1 ggattcttgt tttcgagggg aaagcgggcc cagactccag aagtcttgaa gccgaaattg
       61 ggcacgattc tgcctgcgtg ctcccaccca ttgctatttc tagcctcttc gaggctgtct
      121 gcttctcgca ggatttcgat gtcaggtctt tccgcttctt tgccgcatgc cgcgccaaac
      181 agaatcaaaa gtagaatcaa atttctcaat tgccacccgc ctgaactggc gcatgctgaa
      241 tctgatttcg gccggcttgc tttgcgcgat agagcatgtc gtctgcgttc ttgaacaact
      301 cccgcgctga aatccggggc tccccgtgaa tatccgggtg atggacggca acacctatgc
      361 tgatggtcac gtgatcagcg gtcggcgatt gagcatgagg aatcccggca gagctaatgg
      421 ctcgtcggag atgttctgct ctttctacgc cctctgacag ggttagatct ggaagtatga
      481 caacgaattc ttctccgcca tagcgtgctg tgaattcgcc gccacgtctg aaatgcgact
      541 gaagaatgtt ggaaatagca atcaggcaat cgtcaccggc ctgatgcccg tagtggtcat
      601 tgaacatttt gaaatgatcc acatcgcaga caaggagtgt tacaggttgg ccgtgtcttt
      661 gtgcacgacg gatttcctcc tcaattcgaa cgtcaaggga tcttctattg tagaggccag
      721 taagggaatc ggtggtggct gcctgtgcaa gctgagtcac cgcatgctcc agctcctgat
      781 tggctgcgat cagatcctgt tgcatctgtc gccgctccga aatgtcgcgg ataattccaa
      841 tgaacagcaa ctgaccttct gcgcgtagtt cgcttacttt caagtcaatg ggaaattttg
      901 tgccgtcttt tctgcatgct tccgcttcgc gagccatgcc gattacgttt ttcactcctg
      961 tagccacata gtttgccagg tatacatcat gcatggaatg atgcggttct ggcatcaaga
     1021 cgctgatatt ctggcctgca agttctccgt ccctgtatcc aaagatggca gatcctgcgg
     1081 ggttgatgga ttgaatggtt cctctggtat ctatgatgat gagaccgtcg agcaaggaat
     1141 caaatacttc gcgaagccgg ctttctctat ctttgagtgc aatttcagtt tcgtttactc
     1201 gcgcaaacaa atcgtccaca tcccgtccaa gttctgcgat ttcatccgcg ccttcgagtc
     1261 gtagacgaac gcttctgttt ccacttgcaa atttcttgat tccggttgaa atagtttccg
     1321 ttctccgagt gactcgcatc gtgagcacga gccacaatgc aaacgcacct gcaagcaggc
     1381 caatggcagt gacagtcatc tgcgtccgta gttcgcgatt tgtttcgcgg attcggtacg
     1441 caaggtccag ctcgtagtac acaaacccgc atcgccctcc gcgcagcccg tttctgcctg
     1501 gagcgcacat gcctgcataa ccgactacaa aatcagtccc cgcgtccggg aggatgcgcg
     1561 ggcccgaagc cagagggtct tcaagaaact tcgcgtgaac acgggggtag cgattggcga
     1621 cgagctcgcc tacgtcctga atatttgtgg aagcaatgat cttcccatcc acatcggatg
     1681 cgaagaggac agaaagatct gtttctgagg caaagctgat catggcggct cgcaagggct
     1741 caatgttcgt ggtccgaaaa tagacttccc ccgctgattc gaggaacgcc agcctttctg
     1801 caatcttgat tctactttca cgcgtgagtc tgcccatgcg cactgtgcgc gcagcagcgt
     1861 acgctacgaa gagtgagctc aaggcaaacg aaacggcaag tgcaaggatc aagaaacgaa
     1921 ccgtgcgaaa tcgtttcatt tctctggcgc tctctttacg aagctgtcgt ctgcgattcc
     1981 tcgcgtgtca ggagcttttc tgagcaattc tttctgggcc atgatcttgc ccagtcgcgc
     2041 tgcgtgttct tcaagtggtc cggaaagctc gcgaatattt tctgatctgt cagggatgtg
     2101 aatgccggcg taggcctgtc tggtttcccc gggtgtgagc ttaagttgat tggccacgcg
     2161 agacagaagg gacgcatcgt gctgtatatg tctgtttact tcgaaccatc catccactac
     2221 gttttgtaaa tcgtcgctat gctcctcgat tgcgtccctg cgcgcaatga gtacgtccag
     2281 aatctcgccg ggtatctcgc ggcttgaaaa tatttcgcga gctcccgctt tcaagaggcg
     2341 ggacctctgt ggttcaaatg tgacaactgc gtctactcgt ccctcactga aggcttcctc
     2401 gtgttcattg atcctgattg ggacaattgt gatgtccgcg ctcgtcattt tattcttttc
     2461 aagagcccgt gtgagaacga atgcgccaag cgcggatgct tccactccga ttctttttcc
     2521 acgcagccct tttacatccg caatgagtgg tcgggctaca attgcgtctg ctccctggga
     2581 aatgtcatga atcagtatga cggaaaatgg aatgccactt tcgcgcacca tcaaagcctc
     2641 atccagggtc agggaagcgc actcgagagc gccgccctgg aatgcgcgca gcacttccgt
     2701 agccgaagga tattcgacca ggcgaatgtt tcctggttcc catcccttcc ggtcccgcgc
     2761 cagatagaga ggagcgtatc ctggccaaat atttgttccg atccgcaggg acttacccgg
     2821 ctcgcagtcg agaaagaaca acatggcaca gactgcggcg gcgcaccgga gcataagaca
     2881 gtatgttttt cccctgggaa accacatcaa ctttttgacc gatcggcctt aaagtattgc
     2941 ctgccgctgc tgcggggggt aatattgtga aacgaatgga cccggtcttc gagagaacgt
     3001 gcgaccttat agatggtgag ctggagaact ggcttcaggc agtgctgggg gatattctaa
     3061 cctcgtatga tcgccttcat ttgcgtgatc ctctttgtac ctgtctcctt gagcttgcga
     3121 ccaacgcaaa caaagcgaac atgaagcaca tttttttcct ggagaagggc tgggatattt
     3181 atgatccgca attgtataca gagagactga aagagtttcg cgatcatgtc ctgcactttg
     3241 aaagtacggt cgcatacgcg gaaaaagcga agcaagctgg cctgtacgtt cgtgtttcca
     3301 ttcatcattc gcctgccggt ttgaagattg cagtgtacaa caacttaccg gtcataccgg
     3361 aagacgaaaa aaggattcgt gaaaagtttt tgcacgctct ggagtatcag aatctggtcg
     3421 acttctatcg tgattacgga gacaatagcg agggctcagg tcttggattt gcgctgatgg
     3481 ttttgatgct tcgaagcgaa ggcatcccgc cgtctctttt gagggtcgga acggatggcg
     3541 gtcagactgc tgccagaatc gaagtaccct tccatccggg gagcgctcct cagtctcagt
     3601 gaaagaactt aaccagtgtt acgcgattgc cgcgcgtatt gtattgcacc agatcaaata
     3661 tcgccagagt catcttgatg ccgcgtccat gttctagcct ttcttcattt gcctgatctg
     3721 ctcttgcttt tgcggattcg tgatcaaatc cgcggccttc gtcctccacc tggaatatga
     3781 ctcgatttgg attgagtgaa acatccactt tcacttttct acccgcataa gcaggatccc
     3841 tttggcgggc cagaagcaaa tcgaagtaag caccctgcgc catagcgcgc gtcttcgtat
     3901 cgaagtcgat ttccaggttt ccgtgctcga ctgcattgag taccatttct ctgacagcca
     3961 tcttgatttg ctgtgaatct tcttcgctgc agtatcgtcg caggttgcgc gatattctgt
     4021 gcgaagcgag ctctgaggtg ctgatgaggt tatccacata gtacctggtt ctttctgaat
     4081 agaagaaagc ttgcagcgga tcgtctgcgg gactctgcgc cttgccaaga atggttccgt
     4141 catccctcaa aagttcgaga cgcacctgca agtctcgcga ttctccgagg ctagttttga
     4201 atgcctgcct gaattcagta tggcctctgg tttccagctc ctttagctga aaagtaagta
     4261 gctcgccttt ccaggaacgc gcggtgtaag tgatgtcctg caatctttgc ccaatgattt
     4321 tctttggaga gtagcccaga tgagatccga tggcgcgatt gactgtcagg aatgcaaagt
     4381 ggcgatcgag agagaaaatg atgtcctggg tatcttccac cagttttctg tatttctgct
     4441 cggaaagctc gatggctgct ttcattgttt cgagttcttc aacgtgcttc ttaagattcg
     4501 acctcagtac ctgcactcgg tcagcgagag caagggaaaa tacgacggcc tggatcattc
     4561 ctccaaattg aatgccgtat aacgtcatat tattggcagg aagatatccc cagttgcgaa
     4621 acgtgaatac aattcctcct atcacataga ccacaacagc cagagtgaag agtcgagccg
     4681 ggcgaaagcc agacatcatc actcgaattc cggcgatgag catggtgagg aggcatgggg
     4741 tggccatggc cgccgcgatt tttcccgttg tctgaggttc taccagaagg aaagatagac
     4801 tcgaagcaag tgctatgatt ccagagaatt caatccagcg attgagccag ggtgcagtcg
     4861 tg
//
LOCUS       DAOQJL010000277         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10304_length_4858_cov_4.600666, whole genome shotgun sequence.
ACCESSION   DAOQJL010000277 DAOQJL010000000
VERSION     DAOQJL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10304_length_4858_cov_4.600666"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLG78_12070"
     CDS             complement(<1..438)
                     /locus_tag="PLG78_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23900.1"
                     /translation="MNARLCRSGLKVGIALVIIFICFAATPQPGREEAAESQPLATQL
                     KVKKVEWNLDTFCAHVWIENQSKSPVDRLIIEIGADSLRPVRTTWNRSGSEAVLAPHA
                     ERSFEICDSSLASRKEQLTARVIMASAVIDLAKYSDLCDYIRRC"
     gene            complement(435..1112)
                     /locus_tag="PLG78_12075"
     CDS             complement(435..1112)
                     /locus_tag="PLG78_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE23901.1"
                     /translation="MAAKKIQTTRKKIETEKGSISVCIRRPSSFNVCLIIANGAGAPM
                     DSPFIRHFCDSLAEDGVMTVSFNFPYQEKKRKIPDRQAVLEQTYLSVIDLVARESKLA
                     LSHIVIGGKSMGGRIATHIAPRANVGGLVLLGYPLHAVGKPDVVRDEHLYSIHVPCLF
                     VSGDRDSLCTLALMKKVVSKMKRAAIFVVEDGDHSFKIRKNPARTQEHVFADVREQIL
                     LFLKKFK"
     gene            complement(1114..1626)
                     /locus_tag="PLG78_12080"
     CDS             complement(1114..1626)
                     /locus_tag="PLG78_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNE23902.1"
                     /translation="MNRIDLVLRLLMVALGLSILFLVNQSILKKEDTIRNGAVILLPL
                     APVDPRSLMQGDYMRLNYKLSIVRSVEESAGLVQDGILAVSMEKQIAVSDRRIQSLSE
                     LRKGELPLRFRQRGRVLKFGAESFFFQEGTGEDYAKARFGKLRVDSDGNPILVALCDA
                     SGKEMGFALK"
     gene            complement(1623..2663)
                     /locus_tag="PLG78_12085"
     CDS             complement(1623..2663)
                     /locus_tag="PLG78_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HNE23903.1"
                     /translation="MKLIDILPSVSEEQIRALEGEFAPWYVRLLSGVVSWVAAALILG
                     FLLAAHMLKTDSSLVIYGFLLMGAACVLSRIGRNVAMEQGALAASFSGQVLFLMGVYE
                     LLGHPRESGVVFYVGSAMEIILVFANADRLHRFLVTGLALTFFFVGWGFANHGLSGIG
                     LCIVWAVGGVLFWLISGDGIPAFWFYFLRPAGVASFIVLPLLAFSEWQAPSGDFAYTH
                     QVLTGIATVLLIVFLYVRLANQRAFLVVSCLFVLALAWITSSSPAIMLAAFMIGTAFH
                     ARSLPLLGYSMILLGLGVFQFYYEMGVSLLIKSYILMGSGLVLAAAWMVLRYLVGPES
                     SEEEKDKSGVEV"
     gene            complement(2660..3604)
                     /locus_tag="PLG78_12090"
     CDS             complement(2660..3604)
                     /locus_tag="PLG78_12090"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNE23904.1"
                     /translation="MKATPENAALLHVRGLADRDAYSRALEIMRPTPANWQSFLRRAL
                     LLIAVVLFVAGVIFFFAFNWKDLPRLARLGLVQALFAMFIAGFFFSRGVVRGAAATGA
                     SLTVGAIFAVFGQIYQTGADSYLLFEMWAICAFFIAVFSRFSGSWLIWLIVTNLAVYF
                     YFEQMFAFGHDLPRWLSHSAICAIAWIWFEFQHMRKLEISPHPWLADTAMLGMIAISS
                     LGATMRVFDSGTDPDASHTIPVFLFLSAVAFTASFFYLKRLFGIAVVLGGWILFLTSA
                     MAKNHNTSADFLALAFFLIVQTTLAILFLRYAARRLQS"
     gene            complement(3922..4425)
                     /locus_tag="PLG78_12095"
     CDS             complement(3922..4425)
                     /locus_tag="PLG78_12095"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNE23905.1"
                     /translation="MTEPHKSLTLFYSWQSDLPANTNRGFIEKALQTAIEEIAQDHKI
                     KITIYQDSRESANPEALSKVLGQIDHSAVFVADVTPVDKSRGHLLPNPNVMFELGYAM
                     KSLGADRIIAVHNDFFGDTRFLPFDLGFQRQTTYNLSPEAELKSEERKPLAKQIASSL
                     RTILSLT"
     gene            complement(4532..>4858)
                     /locus_tag="PLG78_12100"
     CDS             complement(4532..>4858)
                     /locus_tag="PLG78_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23906.1"
                     /translation="EASHMFIANSAGLIPFPMSLDFSTTGTDVFWSGMNGNMTAATQS
                     STPAQNAGDPSYRDNCAGWTYQNAPVNPFPTYYANTWTKNSTGTITVATNVACTSTQK
                     IICVQQ"
BASE COUNT         1345 a         1285 c         1169 g         1059 t
ORIGIN      
        1 gcatcttcgt atataatcgc agagatccga atacttcgca agatctatga cagctgacgc
       61 catgatcact ctggccgtca gttgctcctt tctggatgct agagaggaat cgcaaatttc
      121 aaaagatctc tctgcatgcg gagcaagaac tgcctcagag ccgctgcgat tccaggtagt
      181 tcgcacaggg cggagcgaat ccgcccctat ttcaatgatg agcctgtcaa ccggggattt
      241 cgattggttt tcaatccaca catgagcgca gaatgtatcc agattccact ccaccttttt
      301 gactttaagt tgcgtagcca gcggctgcga ttctgccgct tcttctctcc ccggctgagg
      361 agtggctgca aaacaaatga aaatgatcac gagtgcaatc ccaaccttca gacctgagcg
      421 acaaagacgt gcattcattt gaatttcttc agaaataaaa ggatttgctc tcgcacgtca
      481 gcaaaaacgt gttcctgcgt tcgagccgga ttcttccgga tcttaaaaga atgatctccg
      541 tcctctacca caaaaatagc ggcgcgcttc atctttgaaa ctaccttctt catgagcgcc
      601 agagtacaca gtgaatctcg atcgccggaa acaaacagac agggaacgtg gatggaatag
      661 agatgctcgt ccctcaccac gtcgggctta ccgactgcgt ggagcggata tccaaggagc
      721 accaggcctc ccacattcgc gcgaggcgcg atatgcgttg cgattctacc tcccatggac
      781 ttgccgccga tcacaatgtg cgagagcgcg agctttgact ccctggcgac caaatcaatg
      841 acggatagat acgtctgttc cagtactgcc tgccgatccg gtattttgcg tttcttttcc
      901 tgataaggaa aattgaaaga tacagtcatc actccatctt ctgcgagcga atcgcaaaag
      961 tgccggatga aaggactatc catgggagcg ccagcaccgt tggcgatgat caaacagacg
     1021 ttgaaggaag atgggcgacg aatgcaaaca gagatagatc ccttctctgt ttctattttc
     1081 tttcgggttg tttggatctt tttcgcagcc atcttatttc agtgcaaacc ccatttcttt
     1141 tccagacgca tcacaaagag ccactagaat tggattgccg tctgaatcca cacgcaactt
     1201 tccgaatctt gccttcgcgt aatcctcgcc tgttccttcc tgaaagaaaa aagactccgc
     1261 accgaatttc agcacgcgac ctctttgcct gaagcgaaga ggaagctctc ccttgcgcaa
     1321 ttcagacagg ctttgtattc ttctgtccga aaccgcgatt tgcttttcca tggatactgc
     1381 aagaattccg tcctgcacca gtcctgcgct ttcctcgaca gaccgaacta tactgagctt
     1441 gtagttgagc ctcatataat cgccttgcat gagagaacgc ggatccaccg gtgcaagggg
     1501 aagcaagatc accgccccgt ttcgaatcgt gtcttccttc ttcagaatcg attgattcac
     1561 gagaaataga atcgaaagac ccagagccac cataagcagg cgaaggacaa ggtcgatgcg
     1621 attcatactt ccactcctga cttatctttt tcttcctcgg atgattccgg cccgacgagg
     1681 tatcgcaata ccatccaggc cgcggcgaga actagcccgc ttcccatcag gatataagac
     1741 ttaatcaaca gcgacactcc catctcgtag taaaactgaa agactcccag cccgagcagg
     1801 atcattgaat atccgagaag tgggagcgac ctcgcatgaa atgcagtgcc gatcatgaat
     1861 gccgcaagca tgatcgccgg tgaagaagat gtgatccagg caagtgcaag aacaaagagg
     1921 caggagacta cgagaaacgc tctttgattc gcaagcctca catataaaaa cacaatgaga
     1981 agcacggtgg caattccagt caaaacctga tgagtgtaag caaagtcgcc tgacggggcc
     2041 tgccactctg aaaatgcaag caacggcaaa acaatgaacg aagcgactcc tgctggcctc
     2101 aggaaataaa accagaaagc cggaattcca tccccggaaa tcaaccagaa caaaactcca
     2161 cccacggccc agacaatgca aaggccgatc ccggataggc catggttcgc aaacccccag
     2221 ccgacgaaaa agaaagtgag ggcaagacca gtgacaagaa atcgatgcag cctgtctgcg
     2281 tttgcgaata caagaatgat ttccattgca cttccaacat agaacacaac tccggattct
     2341 cgaggatggc ccagcagctc atagacaccc atcaagaaca aaacctgccc ggagaagctt
     2401 gcggcaagtg caccctgttc cattgcgaca tttcgtccga tgcgagacaa aacgcatgcg
     2461 gctcccatga gaaggaaacc gtagatcacc agagatgagt ccgtcttgag catatgagcc
     2521 gcaagcaaga atccgagaat cagggccgcc gctacccagg atacaacacc gctgagcaaa
     2581 cgaacatacc atggagcaaa ctcaccttcc agagctcgga tctgttcttc ggaaacagat
     2641 ggcagaatgt cgatgagttt catgactgta acctcctcgc cgcataacgt agaaatagga
     2701 tggccagggt tgtttgaacg atgagaaaga aggcaagcgc aagaaaatcc gcggatgtat
     2761 tgtggttctt tgccatggca ctcgttagaa agaggatcca tccacccagg accactgcaa
     2821 tcccgaagag cctcttcaaa tagaagaaac tcgcggtaaa tgcgaccgcg gataaaaaca
     2881 gaaaaacagg aatggtgtgg cttgcgtctg gatccgtacc cgaatcaaac actctcatgg
     2941 ttgctcccag agaagaaatc gcaatcattc ctaacatggc cgtatccgcg agccacggat
     3001 gaggcgaaat ctccagtttg cgcatgtgtt ggaactcaaa ccagatccat gcaatggcac
     3061 agatggctga gtgcgaaagc cacctgggta gatcgtgtcc aaaggcaaac atctgctcaa
     3121 aataaaaata cacggcaagg tttgttacga tcaaccatat gagccacgat ccagaaaacc
     3181 gcgaaaagac tgcgatgaag aaagcacaga tcgcccacat ctcgaagagc aggtaggaat
     3241 ccgcgccggt ctggtagatt tgcccgaaca ctgcgaaaat cgcgcccacc gtaagagaag
     3301 ctcctgtggc cgctgcccct cgaaccacac cacgcgagaa gaaaaatccc gcgatgaaca
     3361 tggcgaagag cgcttgcacc agtccaagcc tggccaggcg aggaaggtcc ttccagttga
     3421 aggcgaaaaa gaaaatcacg cccgcaacga acaggacaac tgcaatcagt agcaatgcgc
     3481 gacgcaagaa actctgccag tttgccgggg taggcctcat gatttcgagc gcgcggctat
     3541 aggcgtctct atccgccagg cctcgcacat gaagaagcgc cgcattttca ggagtggctt
     3601 tcattgcggg gaatcaccgc acacaggtcc gcaaaatcaa tcccaaaatc ggacaaaatt
     3661 caatctcatt cccccggcgg tccgcaaaag atcgacaaat gaggccgtca taaatacgta
     3721 gcatggttga aatgcgctga atgtaagaaa actgtattga aatgcccccg atgcggagcc
     3781 acgggatgcc tttctccaaa atgctcaaac aaaggattca aggcaaatgg tcattgcctg
     3841 atgtgcggaa gcgccggaaa accggtggta gacactgaaa cggaaaagtc ctgaactcac
     3901 aggactttcg ctgcaaatgt cttacgtaag acttagtatt gtgcgaagac tcgatgcaat
     3961 ctgctttgcc agaggttttc tctcttcgct cttgagctct gcctccggcg acagattgta
     4021 ggttgtctgc ctttgaaacc caagatcaaa aggaagaaac cgggtatctc caaaaaagtc
     4081 attgtggact gcgatgatgc gatccgcgcc cagcgacttc atcgcatagc ccagctcaaa
     4141 catgacgttt ggattgggga gtaaatgtcc tctgcttttg tccacgggcg ttacatccgc
     4201 gacaaacacc gcagagtgat caatctgacc aagcactttg ctcagcgctt ctggattcgc
     4261 agattctcta gaatcctggt aaatggtgat cttgatttta tgatcctggg caatctcttc
     4321 gatcgcagtc tgtagagcct tttcaatgaa accgcgattc gtgtttgcag gtaagtctga
     4381 ctgccaggaa taaaacaatg tcagcgattt gtgaggctca gtcatgaagc actgtctata
     4441 acggaacgct agagtcaacc cattcggttg atcggctcgc atggagaacg cgggctgcta
     4501 cgcccgcgtt tgtgcctttt cgccgcgaat cttactgctg cacgcagatg attttttgcg
     4561 tgctcgtgca tgcaacgttg gttgccaccg taatcgttcc tgtcgaattc ttcgtccacg
     4621 tgtttgcgta gtacgttggg aacggattga ccggagcgtt ctggtacgtc catccagcac
     4681 agttgtctct ataagatgga tctcccgcat tctgcgcagg agtagaagac tgagtggccg
     4741 cagtcatatt cccattcatg ccactccaga acacatctgt gcctgtcgtg gaaaaatcca
     4801 gggacattgg gaacggaatg agtcctgcag agttcgcaat gaacatgtgg gacgcctc
//
LOCUS       DAOQJL010000278         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10305_length_4858_cov_4.142619, whole genome shotgun sequence.
ACCESSION   DAOQJL010000278 DAOQJL010000000
VERSION     DAOQJL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10305_length_4858_cov_4.142619"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..686)
                     /locus_tag="PLG78_12105"
     CDS             complement(30..686)
                     /locus_tag="PLG78_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE23907.1"
                     /translation="MNIPWKQIASELEDAILILDPSLGIQFINQAASRVFGYTEQDVL
                     RKPIDFLLESETMRKHSEYLARHPESVPTETMPPHTGVRVKRKDGELLEANIIVTRLT
                     SESGTHYLATVRPCEEERPNKSNSAEFAQHVLDAIDAHVAIMDSSGTIVAVNSAWRRF
                     AEQNDGRDVTEGANYLLVCDRASADDRAVEEIASGIRKLLQNGGAHFDMSTRAIPGEG
                     "
     gene            complement(683..1705)
                     /locus_tag="PLG78_12110"
     CDS             complement(683..1705)
                     /locus_tag="PLG78_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE23908.1"
                     /translation="MKASILVIEDEVALRENICELLTLEGYGISEATDGIQGLEEAVQ
                     RHPDLILCDILMPRMNGKEFLAEARRRRELQDTPIVFLTALASSGEVRAGMNLGADDY
                     ITKPFSADDLIVTIETRLDRKRRSMQSIQSRVEKILWRFGDQTSADLNDPLNAIVLPA
                     RVLAERNQNLEKDDIRVLARGILNASSELSRNIDHLLWQQYLDQKIQSWQDDSFKLTT
                     EWILSTLRSTLCDGDTFQIQGHVDITVRGSEEILRQILREAAFIALFEAEAKPLIVSI
                     STESDHVVLRMGCPRGVEAQEANKRAEQEGEKIIATLLESCGGQYERKTINQYDHLTL
                     LLRLVP"
     gene            complement(1702..4152)
                     /locus_tag="PLG78_12115"
     CDS             complement(1702..4152)
                     /locus_tag="PLG78_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE23909.1"
                     /translation="MSDFSKSVFKYIAGIAVTIFLCVFLVFWLIYSRTLQAQEEELSR
                     IVAQEARALSVNGTLIRTATGRDTTPEEIQRLLAVTKEGTGFEIVVGRDVGDRFSIFA
                     SSERGFESLDLPWTSDRAVPMKRALKGETGHDSTVDYEGKEVIAAYAPVPDLHLGLVA
                     KMDRSVIRELYFYSVILSILLAAALSILVSVIVWRVSRISLEKRILRPTLSRYELMLN
                     RSPHCIHEMGPNGAFISMNDAGLRMIRKRKEEIEGLHCLDLVHHSDQARVKMLFERAL
                     KGEPVEFEFKMVSGKSARSDFTPWKNEAGKVTRIIGTTVDLSDELQAAENVRTLENLL
                     METERISQTGSWSLDLKTGELVWSREIYHIFEIEPEFEPSYEEFVNSIHPEDREFVNT
                     AYRESVSSRQQFDVTHRLLMTDGRTKYVREHGITYYDGEGAAVRSVGTVQDITARIAA
                     ETKLAQSEAKYRLLFESSPDAIQVVDPVKLTRLEFNDVSAARLGYTREEYASIKVGNL
                     SDPTTAETFIQNIIRDGEVGAKGSIRKKDGGLLHVHTMAKKIEINGQTYLYMISRDIT
                     AEENALSQIAENLEKEKELNRLRSDFVSMISHQFRTPLATMHSSLQIMQIYSKNGHPP
                     EKMNDHLTRIGNEILRLTQLTEDVLTMGKYAAGAFEYKPQVSDIGCAIGEILAQRFHV
                     LYPQRQCQLERVGQPRKSVFDSVLMTHIFTNLISNAYQYSSGPPRVVVNYVPDEVFVD
                     VIDDGIGISEEDLGRIFRPFFRGHNALAASGTGLGLAIVQQFLEMHGASISVQSELNH
                     GTTFRVHIPDTQEWRTHL"
     gene            complement(4242..4858)
                     /locus_tag="PLG78_12120"
                     /pseudo
     CDS             complement(4242..4858)
                     /locus_tag="PLG78_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453255.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
BASE COUNT         1100 a         1204 c         1182 g         1372 t
ORIGIN      
        1 gaagggaatt gcgtgcattg tataccaggt catccctctc ctggaatggc acgggtactc
       61 atgtcgaaat gggcgcctcc attctgcagc agtttgcgaa tgccggacgc gatttcttct
      121 actgcgcggt cgtccgcgga tgctctgtca catacaagga gatagttggc tccctctgtg
      181 acatcacgcc catcgttttg ttctgcaaat ctgcgccatg cggaattgac ggcaacaatc
      241 gttccgctgg agtccatgat cgccacgtga gcgtcaattg cgtccagaac atgttgcgcg
      301 aattctgcgc tgtttgattt gtttggacgc tcttcctcgc acggtcgaac ggttgccagg
      361 tagtgcgttc cactctccga ggtgagccgg gtaacaatga tattcgcttc gaggagctca
      421 ccgtcttttc gtttaactct cactcctgta tgtggaggca tcgtttctgt ggggacggat
      481 tccgggtggc gcgcgagata ttcagagtgt ttcctcatgg tctccgattc cagtagaaag
      541 tcgattggtt ttcggagaac gtcctgctcc gtgtacccaa acactctcga tgctgcctga
      601 ttgataaact gaataccgag tgaggggtcc agaatgagga ttgcatcctc caattcagag
      661 gcgatctgtt tccatggtat gttcacggaa ctaaccgcaa caaaagtgtt aggtgatcgt
      721 attgattgat tgtctttctt tcgtactgac ctccgcagga ttcaagaaga gttgctatga
      781 tcttttctcc ctcttgttct gctcgtttgt tcgcctcttg agcttccacg ccgcgcggac
      841 aacccattcg cagcacgacg tggtcggatt ctgtagatat ggaaacaatg agtggttttg
      901 cttcagcctc aaagagggcg atgaaagcag cttctctcaa tatttgtcgc aatatttctt
      961 cgctgccgcg taccgtgatg tctacatggc cttgaatttg aaatgtatcc ccatcgcaga
     1021 gagtactgcg caaggtgctc aggatccact ctgtcgtcag tttgaaagag tcatcttgcc
     1081 atgattgtat tttttgatcc aggtattgtt gccacagaag gtgatctata ttccggctaa
     1141 gttcggatga agcattgaga atcccgcgag cgagaacgcg gatatcatct ttttcaagat
     1201 tctgatttct ctctgccagt actcgcgcgg gtaaaacgat ggcattgagt ggatcgttca
     1261 agtccgcaga tgtctgatct ccaaatcgcc acaaaatttt ttccacgcgc gattggatac
     1321 tttgcatact acgacgtttg cgatccaatc ttgtttcaat ggtgacaatc aagtcgtcgg
     1381 cagagaaggg ttttgtgata taatcatccg ctccaaggtt catcccagcg cgcacttctc
     1441 cagaggaggc aagcgcagtt agaaatacaa tcggtgtatc ctgaagttcg cgcctgcgtc
     1501 ttgcctctgc gagaaattcc ttcccgttca tccgaggcat gagaatgtcg catagaatga
     1561 gatccggatg gcgctgcact gcttcttcga gtccttggat tccatcggtg gcttcgctga
     1621 taccatagcc ttcgagcgtg agtagttcac atatattttc gcgcagcgct acttcgtctt
     1681 cgatcactag tatactcgct ttcacaggtg ggttctccac tcttgtgtgt ccgggatgtg
     1741 gacgcgaaat gtggtgccgt gattgagttc actctgaacc gagatactcg ctccgtgcat
     1801 ttccaaaaat tgttgaacaa tcgccagccc gagcccggta ccagaggcag cgagtgcgtt
     1861 gtgtccgcga aaaaagggtc gaaaaatcct tccgagatcc tcttccgaaa tcccaatacc
     1921 atcgtcgatc acgtccacga atacttcatc tggaacgtag tttaccacaa ctcggggggg
     1981 tcctgatgaa tactgatacg cgttagaaat caggttcgtg aatatgtggg tcatgagaac
     2041 agaatcgaac acactcttgc gcggttgccc cacgcgttcc aactgacatt gtctttgtgg
     2101 atagagcacg tgaaatcttt gtgcgagaat ttctccgatc gcgcaaccga tgtcagagac
     2161 ctgcggtttg tattcgaagg cgcccgctgc atactttccc atggtcagta cgtcttctgt
     2221 gagctgtgtg agtcgcagga tttcgtttcc aattcgcgtc aggtgatcat tcattttttc
     2281 tggtggatga ccgtttttgg agtagatttg catgatctga agactggaat gcatagtggc
     2341 cagcggcgtg cgaaattgat gtgagatcat ggatacaaaa tcagatctaa gccgattgag
     2401 ttctttttcc ttttccagat tttctgcgat ctgcgaaaga gcattttcct ctgccgtgat
     2461 gtcgcggctg atcatataca aataggtctg cccattgatt tcgatctttt ttgccatcgt
     2521 atgaacgtgc agcagcccgc cgtccttctt tcgaatcgat ccttttgcac ccacctcgcc
     2581 gtctcgaatg atgttctgga tgaaagtttc cgccgtggtg gggtcgctca gattgcccac
     2641 tttgatggac gcatattctt cgcgggtgta gccgaggcgt gctgcggaca catcgttgaa
     2701 ctctaatcgc gtgagcttga ctggatcaac cacctggata gcatctggag atgactcaaa
     2761 aagcagtctg tactttgctt cgctctgcgc gagttttgtc tctgcggcaa tccttgctgt
     2821 gatgtcttgc acagtcccaa cagatcgcac tgcggctccc tctccatcat agtacgtaat
     2881 tccgtgttct cttacatact tggtgcggcc atccgtcatg aggagcctgt gagtgacatc
     2941 aaattgctga cgtgacgaaa ctgattccct gtacgccgtg tttacgaact ctctatcttc
     3001 tggatgaatg ctgtttacga attcttcgta agatggttca aattcgggct cgatttcaaa
     3061 gatgtggtaa atctccctgg accagacgag ttcgcctgtc ttcaggtcca gggaccagct
     3121 tcccgtctgg ctgatgcgtt ctgtttccat caacagattc tcaagcgtcc gcacgttttc
     3181 ggcggcctga agttcatccg acaaatcaac agtagttcca atgatgcgcg tgactttgcc
     3241 cgcttcattt ttccacggag tgaagtctga gcgtgcgctt tttcctgaaa ccattttgaa
     3301 ttcgaattcg acaggttcac ctttgagagc gcgttcgaag agcatcttga ccctcgcttg
     3361 atcggaatgg tgaaccagat caagacaatg aagtccctca atttcttctt ttcgctttcg
     3421 aatcattcgt agcccggcat cgttcatgga aataaacgcg ccgttggggc ccatctcgtg
     3481 aatgcaatga ggagaccgat tcagcatgag ttcatagcgc gagagagtgg gtcgtagaat
     3541 ccgtttttct aacgaaatgc gcgaaacgcg ccaaacaatg acactcacaa gaatagaaag
     3601 cgctgcagcc agcaagatgc tcaggatgac agaatagaaa tacagttcgc gaatcacact
     3661 gcgatccatc ttggcaacca gacccagatg aaggtctggc acaggtgcat aggctgcgat
     3721 cacctctttg ccttcatagt ccacggtgga atcatgacca gtttcacctt tcagtgctcg
     3781 cttcatagga acggccctgt cgcttgtcca ggggagatcg agagattcaa aacctcgttc
     3841 gctcgatgcg aagatgctga agcgatcgcc cacatcgcga ccgaccacaa tttcaaatcc
     3901 agttccctct ttcgtcacgg cgagtaatct ttggatttcc tcaggtgtag tgtcgcgtcc
     3961 agtggcagta cggatcaagg taccgttgac ggacagggct cgtgcttcct gtgcaacaat
     4021 tcgagacagc tcttcttcct gcgcttgcag cgtacgggaa tagatcagcc agaataccag
     4081 gaaaacacag agaaaaatgg ttaccgcaat tccggcgatg tatttgaaaa ctgatttaga
     4141 aaaatcgctc aacgtgtaaa acgaaggcga aacgctcggg tcttacgagt agaaaacgaa
     4201 tccgtgattg gaaaaaaata tccgtaccac tacggatgcc tttactctgc tttctctccg
     4261 gctttgagcg ccggtcccat tccagcgagg cttttgagat acgcatttac gaacggatct
     4321 agatctccgt ccatgactgc gtctacattg cctgtttcat aatctgtgcg aaggtccttc
     4381 accagtttgt agggatgcat tacatagctt cgaatttgac tgccccatgc tacgtctcgt
     4441 ttttctccgg agcgactttc aatgtcgtct tcgagttttc gtttttctaa ttcgtggagc
     4501 cttgccttga gcattttcat cgcagtagat ttgtttttca tctgcgaacg ttcattctga
     4561 cactgcacaa caattcctgt aggattgtgt gtgattctca cagcggagtc tgttttgttt
     4621 acgtgctgtc cacctgcgcc gctcgaacgt tatgtatcaa tgcgaagatc tttttcatca
     4681 atgttgacaa tgatggaatc atcaatgtcc ggagtcacgt gcacggctgc gaaagaagta
     4741 tgtcttcttt tgtttgaatc aaaaggggaa atccgaacca gtctgtgaac tccattttcc
     4801 gcattcaaaa ggccaaatgc attttcaccg cgaacaatga gcgtggcgtt tttgattc
//
LOCUS       DAOQJL010000279         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10340_length_4847_cov_4.900668, whole genome shotgun sequence.
ACCESSION   DAOQJL010000279 DAOQJL010000000
VERSION     DAOQJL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10340_length_4847_cov_4.900668"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLG78_12125"
     CDS             complement(<1..522)
                     /locus_tag="PLG78_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23910.1"
                     /translation="MDTSRSIERVGGFCCSVLVALYVIAGVTFLLLPVKASVLDPVGF
                     ANAYTTHRFVYRAFFLSFGFIAVFTFGAIPAISALVRSESEGLIRWSSNLAYLGSGVL
                     ALNSFRTVGLIDMRTEIFLSLDAEGQKILAAAPLVMDQHAYLTFGATGFYFLTVGILL
                     FKSEGGLLASVGVM"
     gene            complement(625..1704)
                     /locus_tag="PLG78_12130"
     CDS             complement(625..1704)
                     /locus_tag="PLG78_12130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE23911.1"
                     /translation="MIVRFSKPLRRVIALIAIPALSLSGAAPIGANLHRIPGSAVFLT
                     FDAHPCKDTMLHKCEDLPAIDGAWKLLDTLRANGIRSTFFFTGQFIEKYPDIVRRALQ
                     DGHEIGSHLNSHTHPLEFQEKHITLNKTWFMNELLSADRALVKTGNGHAVKFFRMPYG
                     LASYSVLPGARRNILQWAQEAGYTHVDWSVDTLDWISRDARSHGSFLPPYLTSRQMEE
                     RVIADRGGAIVLSHLTRYRPFGEPSVNEMIPDLLVRLKKQNLRTARISDGLGVVPPAE
                     LAVVKEESVREVQKETPVAAVNKCNYSWDFKRVHICITGMTESDCAGLEESFLSREEE
                     CICTKPGVKYNLQNLGELVQISCKL"
     gene            complement(1701..2924)
                     /locus_tag="PLG78_12135"
     CDS             complement(1701..2924)
                     /locus_tag="PLG78_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23912.1"
                     /translation="MKVPKRLIASLAVAIVLIGVIAYFLLAPSRAQREQAQRESGSQT
                     EPGLRTDENGMPVIDDDPAAHLERYRKWAQYPPNSRPLFAGQVDLLDPYNAEKNPVYV
                     VQTPAEGCSANAQGLLECAKKAVFSDVECKLTPERYISIGTKDFHLYLSCTSKKQNEG
                     KPLAIESIETKFYRQLFKNIIPSLPPIAVGDDGANGDEKAGDHIYTITVRPTSQDWGD
                     MFLEAEFKVAGFKHVQRADFFSTPHKTAEFQSGIRDANRNGQLVVSVPVNVFKAGYYK
                     FDANLVQKEDDHQPVASASWEGDLTPGAQTIDIQFFGKVIKDQKLNGPYVVKNLRGMR
                     NNSPVTPSMLKNAIASGKPIGPQQHKEPLQEYMEPYAGDYSTQAYRADDFSSDEWQSE
                     EKDRRIKYLESLGTN"
     gene            complement(2963..3868)
                     /locus_tag="PLG78_12140"
     CDS             complement(2963..3868)
                     /locus_tag="PLG78_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23913.1"
                     /translation="MTIAADMNLFFARLIALLIMTPFFSCAVLFWKDASNSSGGTWKG
                     NDRFIEIQTEFQRKSSWYPLNQNALTRNYSTTLLLHKIAGESSTSSEKIAHYSGWTLN
                     DTLFLAGSSLIAIRGTSNSLGDENRELLSVSLKEGTVSDASVILKPDAFLLAAVPSPD
                     GKWIAAFLTDATNEKRTGNLFVDFYEFRGSSSLQKKSRVTVQWSGVPGNPELSWSRDS
                     QGLYLHVESAVLYLTAGGSTARNADRFPACFIPTSSGRNISESGLFFGRSQEGQVEIR
                     KTNEGIRFDAIPMVNNINDLGKGCP"
     gene            complement(3953..4822)
                     /locus_tag="PLG78_12145"
     CDS             complement(3953..4822)
                     /locus_tag="PLG78_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23914.1"
                     /translation="MKKLILLGLVAFAASSVSAATFKLFVHGRSATNHCSGTIPNTSS
                     ATTDVGGYWSGWGFTPQTTNVRYVGFDGEQPGGAYSWNECGAQLELYRALNTFCTGAN
                     DCEIYTHSTGGLVASAFFGLNPSLASYYHIRRIQEMASAAGGSELADMSVTYLAWIGW
                     SNYGGQLDHSVSTSGARNGFNHNQSGGLTIYLTSGEGHDPETYGVTPAFLPGIDDGVV
                     ANHSLCGINQVGSVNAHCARGNGQLKESYACGFLWLSTCYRTWTRWTPYYTVWQGGSG
                     ETHKTAMADYNRR"
BASE COUNT         1223 a         1246 c         1172 g         1206 t
ORIGIN      
        1 catcacgccc acagatgcaa gtaaaccgcc ctctgacttg aaaagtaaga tcccaaccgt
       61 gagaaagtaa aagccggtgg cgccaaaggt gagatacgcg tgctgatcca tgacaagcgg
      121 cgctgccgcg agtatctttt ggccttccgc atccaggctc aaaaagattt cagttcgcat
      181 gtcaatgaga cccactgtgc gaaatgaatt caaggccagc acaccagaac caagatatgc
      241 gaggttcgag ctccaccgaa tgagaccctc actttcagag cgcaccagtg cggaaatcgc
      301 agggatcgca ccaaaggtga agactgctat gaaaccgaat gacaaaaaga aagcgcggta
      361 tacaaatctg tgcgttgtgt acgcatttgc aaatccaacg gggtccagaa cagaagcttt
      421 cacaggcaag agcaggaagg tcacacctgc gatgacgtaa agcgccacga gtactgaaca
      481 acaaaaaccg ccgactcgtt caatgctacg acttgtgtcc acaaagcacc tcctggcgcc
      541 cgcgctgctg gcaatcgatt tgctcttcct gaatggacga taagcgcctg aatcaacagt
      601 gcaactcttt ttgactaact taaattacag tttgcaggaa atctgaacca gctcgcccag
      661 attctgcaga ttgtatttca cacctggttt ggtgcaaatg cattcttctt ctctagagag
      721 aaaggactct tccagacccg cgcaatctga ttcagtcatc cctgtaatgc atatgtgaac
      781 acgtttgaaa tcccaggaat aattgcattt gtttactgcc gcaaccggcg tttccttctg
      841 cacttctcgc acagattctt cttttacaac agccagctcc gcgggaggaa ccacacccag
      901 gccatcagaa atccttgcgg tgcgaaggtt ctgctttttc aggcgaacga gcaaatctgg
      961 aatcatttca ttcacggaag gttcaccaaa cggcctgtaa cgcgtcagat gactgagaac
     1021 aatcgcccct ccccgatccg caatcacgcg ctcttccatt tgcctcgatg ttaaataggg
     1081 aggtagaaat gatccgtgcg atctggcgtc cctggagatc caatccagag tgtctacact
     1141 ccaatcgacg tgcgtatatc ctgcctcctg agcccattgc agtatattcc tgcgcgcacc
     1201 cggaagcacc gaatatgacg ctaaaccata tggcatgcga aagaatttca ctgcgtgacc
     1261 atttcccgtc ttcaccagcg cgcgatctgc actcaaaagt tcattcatga accaggtctt
     1321 gtttaacgtt atatgctttt cctgaaattc aagaggatga gtgtgagaat tcagatgcga
     1381 gccaatttca tgaccgtctt gtagggcacg gcgcactatg tccggatact tctcaatgaa
     1441 ttggcccgtg aaaaaaaatg tagaacgaat gccatttgcg cgcagagtat cgagcaactt
     1501 ccacgcgcca tcaatggcag gaaggtcctc gcacttatga agcatcgtgt ccttgcacgg
     1561 atgcgcatca aatgtaagaa atacggcact gccaggaatt cgatgcagat tggcaccaat
     1621 gggagcagcg cccgaaagag aaagcgccgg aattgcaatg agtgcaataa cccggcgcaa
     1681 tggtttagaa aatcgaacta tcaattcgta cccagactct ccagatattt gatacgacga
     1741 tccttttctt cagattgcca ttcgtccgaa gaaaaatcat ctgcacgata cgcctgcgta
     1801 gaataatccc cggcataagg ctccatgtat tcctgaagag gttctttgtg ctgctgagga
     1861 ccgatcggct taccactcgc aattgcattt ttcagcatag aaggcgtaac gggagaattg
     1921 tttcgcattc cgcgaaggtt cttcaccacg taaggtccgt tcaatttctg atccttgatg
     1981 actttgccaa agaattgaat gtcgatcgtt tgcgcgccag gggttaaatc tccttcccag
     2041 gaggcgctgg cgactggttg atgatcgtcc tccttttgca ccaggtttgc atcaaacttg
     2101 tagtagcctg ctttgaacac gttgaccggc acggatacta cgagctgacc gtttctattc
     2161 gcatcccgaa tgccactctg aaactcagca gtcttgtgcg gagtggagaa gaaatctgca
     2221 cgctgcacgt gtttgaatcc tgcgaccttg aattcagctt ccaggaacat atcgccccag
     2281 tcctgagaag taggacgaac tgtgatggtg tagatatgat cgcctgcttt ttcgtctccg
     2341 tttgcgccat catcccccac ggcgatcggc ggcagagatg gaatgatgtt cttgaacaac
     2401 tgacggtaga actttgtttc aatggactca atcgcaagcg gcttcccttc gttttgcttt
     2461 ttggaagtgc acgaaagata cagatgaaag tcttttgtac caatagaaat gtaacgctcc
     2521 ggagtgagct tgcattccac atccgagaac acagcctttt tcgcgcattc cagaagcccc
     2581 tgtgcatttg cgctgcaacc ttctgctgga gtttgcacaa cgtagactgg attcttttct
     2641 gcgttgtatg gatccaggag atccacctga cctgcaaaaa gaggacgaga gtttggtgga
     2701 tactgtgccc acttcctgta tctctcaaga tgggctgccg gatcgtcgtc gatcacaggc
     2761 attccgtttt catccgtgcg aagacctggc tctgtttgcg agccactttc tctttgcgcc
     2821 tgctctctct gcgccctgga cggagccagc aggaagtagg caatcacgcc gatgagcaca
     2881 atggctacgg caagtgacgc aatcaaacgc ttgggcactt tcatacttta tttccccttt
     2941 tttgaacttt ctctttctta gatcaagggc atcccttgcc cagatcgttt atattattta
     3001 ccattgggat cgcatcgaaa cgaattcctt cgttcgtttt tcggatttcc acctgccctt
     3061 cctggctgcg cccaaagaaa agcccggact ctgaaatatt ccgtccggaa gaggttggaa
     3121 taaaacatgc gggaaaacga tctgcattcc tggcagtcga tccgcctgct gtcagataca
     3181 gtactgccga ttccacatga agatacagtc cctggctgtc gcgagaccag gaaagctcag
     3241 gatttccggg cacgccggac cactgcaccg tcactctgct tttcttctgc aaggaagagc
     3301 ttccgcgaaa ttcataaaaa tccacaaata ggtttccagt gcgtttttca ttggttgcat
     3361 ctgttagaaa cgccgcgatc cattttccat ccggagaagg tacggcagcc agaagaaatg
     3421 catccggttt caaaatcaca gaagcgtcag aaaccgttcc ttctttgagg gacacgctca
     3481 gaagttcacg gttttcatcc cccagtgaat tgctggtacc gcgaatcgca atgagagaag
     3541 aacctgccag gaatagagtg tcgtttaacg tccagccaga gtagtgcgcg attttctcag
     3601 acgatgtaga cgattcacct gcgatcttgt ggagcagcag agtggttgaa taattgcgag
     3661 tcagtgcgtt ctgattgagg ggataccagg atgattttct ctgaaattcc gtctgaattt
     3721 caatgaaacg atcgtttccc ttccaggttc cgccggaaga attactcgcg tctttccaga
     3781 agagcaccgc gcaggaaaaa aatggagtca tgatgagtaa agcgatcagt cgtgcaaaaa
     3841 acaaattcat atcagctgcg attgtcatcg ccgagtcaag aacggtccac acaaaaaaaa
     3901 gaccgcgccg aattagacgc ggtctcttgt ttcgcatgga ttctaagttg aatcagcgac
     3961 ggttgtagtc agccattgct gtcttgtgcg tttcgcctga tccgccttgc catacagtgt
     4021 agtatggagt ccagcgagtc caggttctat agcaagtaga gagccacagg aatccacatg
     4081 cataggattc tttgagctgt ccgttgccac gtgcacaatg tgcgtttaca gaaccaacct
     4141 ggttgatacc gcagagagag tggtttgcaa ccacgccgtc gtcgatgcct ggaaggaacg
     4201 caggagtcac tccataagtt tctggatcat gaccttcgcc tgacgtcagg tagatagtga
     4261 gtccaccgga ctggttgtgg ttgaagccgt tacgcgctcc gcttgtgctc actgaatgat
     4321 ccagctgtcc gccgtagttg ctccagccaa tccatgcgag gtaggtaact gacatatcag
     4381 ccagttcaga tccacctgct gcggatgcca tttcctggat gcgacgaatg tggtagtagc
     4441 ttgctagaga cggattgagg ccaaagaaag cagaagctac cagcccacct gttgagtgag
     4501 tgtagatttc gcaatcgttt gcgccagtgc agaatgtgtt gagtgcgcga tacagctcaa
     4561 gctgtgctcc gcattcgttc cagctgtagg ctccgcctgg ttgttcgccg tcaaaaccaa
     4621 cgtaacgaac gtttgtggtc tgaggtgtga atccccagcc gctccagtaa ccgcccacgt
     4681 ctgtagttgc gctggatgtg tttggaatgg tgccactgca atgattcgtt gcagatctac
     4741 cgtgtacgaa aagtttgaat gttgcggctg aaacgctaga tgctgcgaat gccaccagtc
     4801 ctagaagtat aagtttcttc atcggtcact ccccccactg agtgttg
//
LOCUS       DAOQJL010000280         4846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10347_length_4846_cov_3.810269, whole genome shotgun sequence.
ACCESSION   DAOQJL010000280 DAOQJL010000000
VERSION     DAOQJL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10347_length_4846_cov_3.810269"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLG78_12150"
     CDS             complement(<1..242)
                     /locus_tag="PLG78_12150"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE23915.1"
                     /translation="MAAIERFLARKDPELAEGVGLLCNHTSFHFASGQYLFQILSGRG
                     SLRRLFLPEHGLFAELQDQVGLSQTNVYESLGLKCQ"
     gene            284..2299
                     /locus_tag="PLG78_12155"
     CDS             284..2299
                     /locus_tag="PLG78_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE23916.1"
                     /translation="MQLNEEQSRAVATVHGPVLVFAGAGSGKTRVLTQRIQRMIESGV
                     AAAHITAVTFTNKSAREMKERLSKLPREKRRGLIVSTFHSLGARILRSHIEKLGYGNP
                     FTILSEDDRLRVLSEIYSRHKLDPSDAKKDGLLSWFSRAKNALANPARFFESNGLDEG
                     VIDFYEEYEHALRSTNSLDFDDLILLPIRLFHKDQELLEKYRKERTHYLVDEFQDTNP
                     MQYEFLRLLVLPHRNIFAVGDDDQSIYAFRGSDISLILNFHKDFAGASVHRLEKNYRS
                     QAEIVRAASAVIVRNTSRAPKEIRSVRPPGEKILGIYGTDEEEEAALVAEEIRQRIVR
                     ERRKPEDFAVLVRTNFQSRAFENAFRSAGIPHRVVGGYRFFDRREVRDVLSYLRIIAN
                     PRDDLSLIRILSRPGNGLGEVSIARIREASRDTGLYEFLVRMQGEPGLVQLKREGSAF
                     IAELMEIMERHRPAFARGVTQAARALVYELKFDSQFRREGEEDSAVSMRMQNISELLG
                     MMQSLETEARQEGEDFTIFDFLKAVSLLTSDDEEESGGRAQIMTVHVSKGLEFPVVFL
                     SGLMDGLFPPERSLSESGDIEITVAEERRLFYVGMTRAMDVLYLTASRTKKKFGEIID
                     MEPSRFLSELPEDSLVWTRGDPSRKPDPENELTDLISALDSFRAGKP"
     gene            2296..2634
                     /locus_tag="PLG78_12160"
     CDS             2296..2634
                     /locus_tag="PLG78_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23917.1"
                     /translation="MIQLLGTIVITAVVSSLFTLGLSYAFYILHLRDLLDRKLDEKLE
                     LAKSKVKEGLREEALDLMPKFRAEVREGFKEALNSAMGGQIIENMTKPVSSVVEASLS
                     FLIGRQEEER"
     gene            complement(2638..3657)
                     /locus_tag="PLG78_12165"
     CDS             complement(2638..3657)
                     /locus_tag="PLG78_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23918.1"
                     /translation="MRTVILSLLLTLGGPLFAVSPDYSNMGQVLAQNRMYLLFMDTCV
                     VNLRGPSAQEDPKGMRAQFEGYYKAAIEHDFYANLWYLQGNYGKTYSELRLSQNALQD
                     LYRRILENYIDETVILLEASAPIIVLTRDQSAKKLLELGFRDLESTRQIYLRGANTNP
                     KMFLNQIMTYQDGILRARRGRRFAILALLEAKVPVPEKSKYQVVTLDDVKNMMEEGEK
                     QTKYVEILNLLVNMTGRNLVPKVIASDVLGKMISLNTLEVHQDNYGRMYSDRRSVFQL
                     ITVTLRSDEFHKSDSMPRRNTDNRNEVPASDSDPIKPEGTTTQPQPQAITPVTPAPAT
                     DPGKK"
     gene            complement(3704..>4846)
                     /gene="lysA"
                     /locus_tag="PLG78_12170"
     CDS             complement(3704..>4846)
                     /gene="lysA"
                     /locus_tag="PLG78_12170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNE23919.1"
                     /translation="ESALVERCQALGSAFGNRRAGIYYSVKACSNIHILRILAGLGCG
                     ADIVSGGELFRCRKADISPEKIVFSGVGKTEAEIRYAITEGILFLSVESEAELHEISR
                     IATITGKTARISVRVNPDVNPGTHPYISTGLKENKFGISHKEVVRIYKLAQSLPGIEP
                     TGLGFHIGSQITDLSAFADAGKIAVRLVSEIEAAGIKLKHLDVGGGLGIRYANEEAPD
                     PATYVRTLIQSVGLDHLELMFDPGRSIAGNAGILVSKILYVKEGEKTFFIGDAGMNDL
                     IRPSLYDAYHHVFSDPLRPGTRSGDLVGPVCESGDFFAKDRELPAFESGDLAVLASAG
                     AYGMSMASNYNSRPRAAEVLIARDGTARLIRRRETFEDLLQPELDV"
BASE COUNT         1228 a         1209 c         1199 g         1210 t
ORIGIN      
        1 atctggcatt tcaatcccag gctttcatac acatttgttt gcgagagtcc tacctggtcc
       61 tgtagctccg caaaaagtcc gtgctcagga agaaagagcc tccggagaga tccgcggccg
      121 gacaaaatct ggaatagata ctggcctgag gcaaaatgaa acgaagtgtg attgcacaga
      181 aggcccacgc cttcggccag ttccggatct tttctcgcca ggaatctttc gattgccgcc
      241 atgagcggta ttatgtcact cgtcccaatc gtcgagtttt ttcatgcagc tcaatgaaga
      301 acaaagccgc gccgtagcaa ctgtgcacgg accagtactt gtgtttgcgg gtgcgggttc
      361 cggcaaaaca cgcgtcctca ctcagcgaat ccagcgcatg atcgaatcag gtgtcgcggc
      421 cgcgcacatc actgctgtga cgttcacaaa caaaagtgcg cgggagatga aagaaagact
      481 ctccaaactg ccccgcgaaa agcgccgagg cttgatcgta agcacatttc attcacttgg
      541 agcgagaatc cttcgttctc acattgaaaa gcttggatac gggaatccat ttaccatctt
      601 atcagaagac gacaggcttc gcgtgctttc tgaaatctat tcccgccaca agctcgatcc
      661 gtcagacgca aagaaagacg gactcctctc ctggttttcg cgagcgaaaa acgctctggc
      721 caatcccgcg cgtttcttcg aatccaatgg cctggatgaa ggagtgattg atttctatga
      781 ggagtacgag cacgctcttc gcagcaccaa cagccttgac tttgacgatt tgatcctcct
      841 tcccattcgc ctgtttcaca aggatcagga gctccttgag aagtacagaa aggagcgcac
      901 tcactatctg gtggatgagt tccaggatac aaacccaatg caatatgagt ttctgcgcct
      961 tctggtgctg cctcatcgca atattttcgc agtgggagac gatgatcagt ccatctatgc
     1021 atttcgggga tctgacatca gcctgatctt aaattttcac aaagattttg ccggtgcaag
     1081 tgtacatcgg ctggaaaaga actacagatc tcaggcggaa attgtgcgtg ctgcgagtgc
     1141 cgtgattgtt cgaaacacgt ccagggcgcc aaaggaaatc aggagtgttc gtccgcctgg
     1201 agaaaagatt cttggaatat acggaacaga tgaggaagaa gaagcggccc tggttgcaga
     1261 agagatcagg cagagaattg taagagagcg ccgcaaaccg gaagattttg cagttcttgt
     1321 cagaacgaat ttccagtctc gtgcgtttga aaatgcattt agaagtgctg gaattccgca
     1381 cagagtcgtg ggaggatatc ggttttttga caggcgtgag gtgcgcgatg tactttccta
     1441 cctgcgaatc atcgcaaacc ccagagacga tctaagtctg atccgcattt tatccagacc
     1501 tggaaacggc cttggagaag tatccatcgc gcgaattcga gaagcgtcga gagacacagg
     1561 cctctacgaa tttctagttc gcatgcaggg cgagccgggc ctcgtgcaat tgaaaagaga
     1621 aggatctgca ttcatcgcgg agctcatgga aatcatggag cgccatcgcc ccgcttttgc
     1681 cagaggagtc actcaggcag cacgggccct tgtgtacgaa ctcaagtttg attctcaatt
     1741 tcgccgcgaa ggggaagaag attctgccgt ttcgatgcga atgcagaata tttcagaatt
     1801 acttggaatg atgcaatctc tcgaaacgga agcgcggcag gaaggagaag acttcactat
     1861 ctttgatttt ctcaaagcag tttcgcttct cacttcagac gatgaagaag aatcaggtgg
     1921 gcgcgcgcaa atcatgacag tgcacgtatc caagggcctt gagtttccgg tggtatttct
     1981 atccggtctc atggacggtt tattccctcc ggaaagatcc ctcagcgaat cgggagacat
     2041 tgaaatcaca gtggctgaag agcgaaggct cttctacgtt ggaatgactc gcgctatgga
     2101 cgttctgtat ttgaccgcat cgagaacaaa gaaaaagttc ggcgaaatca tagatatgga
     2161 accgtcccgt tttttatcag agctgccgga agactctctc gtatggacca ggggggaccc
     2221 ttctagaaag ccggatccag aaaatgaatt gaccgacctt atatccgcgt tagactcatt
     2281 tcgagcaggt aaaccatgat acaactcctg ggcacaatag taattacggc cgtcgtttcc
     2341 tcattgttca ctcttggact atcctacgca ttctacattc tccaccttcg tgatctgctc
     2401 gacagaaagc tcgacgaaaa actggagctt gcaaaatcaa aagtgaaaga aggcttgaga
     2461 gaagaagctc tcgacctcat gcccaagttt cgcgcggaag tgagagaagg atttaaagaa
     2521 gctctcaact ctgcgatggg cggtcagatc atagagaata tgaccaagcc agtttcgagc
     2581 gtagtagaag caagcctcag ctttttgatt ggtcgccagg aagaagagcg ctgagcgcta
     2641 cttcttcccc ggatcggtag cgggcgctgg agtcacagga gtgattgctt gaggctgtgg
     2701 ctgcgttgtg gttccttccg gcttgattgg atctgaatca gaagcaggca cttcattgcg
     2761 attgtccgtg tttctcctgg gcatggaatc agacttatga aattcatctg atctcagtgt
     2821 tacagtgatc aactgaaaca cacttcgtct gtctgagtac attctgccgt agttgtcctg
     2881 atgcacttct aaagtattca ggctgatcat ctttccaagc acgtcgcttg caatgacctt
     2941 gggcaccagg tttcttccag tcatgttcac aagaaggttc aggatctcta catacttcgt
     3001 ctgcttctct ccctcttcca tcatgttctt cacatcgtcg agagtcacca cctgatactt
     3061 tgatttttca ggtacaggaa cttttgcttc gagaagtgct agaatggcaa atctgcgccc
     3121 acggcgagcg cgaaggatcc cgtcctgata ggtcatgatc tggttaagaa acatctttgg
     3181 attggtgttt gcaccgcgca gataaatctg tctcgtagat tcgagatcac ggaaaccaag
     3241 ttccagcaat ttctttgcgc tttgatcgcg agtcagaacg atgatgggtg cagatgcttc
     3301 gagcaaaatg acagtttcat ctatgtagtt ttccaggatt cttctgtaga gatcctggag
     3361 cgcgttttga gacaatctga gttccgaata ggtctttccg tagttcccct ggagatacca
     3421 tagattcgca tagaaatcgt gctcaatggc tgccttataa tagccttcga attgcgcacg
     3481 cattcctttt ggatcttcct gggcagaagg cccgcgaaga ttgaccacgc atgtatccat
     3541 aaatagaagg tacatgcggt tctgtgcgag aacctgcccc atgttcgaat agtcgggcga
     3601 cacggcaaaa agaggaccac ccagagttaa gagaagagac agaattacag ttctcaccct
     3661 tccgatatcg gccttccacg ccatttttcg agcaaaatac gagctacaca tccagttccg
     3721 gttggagcag atcctcaaag gtttcgcgtc tgcgaatgag ccgggcagtc ccatcgcgcg
     3781 caatcagcac ctctgcggcc ctgggtctgg agttgtagtt agaagccatg ctcattccgt
     3841 atgctcccgc agaagcgagc acggccaggt cccccgactc aaacgcgggc agctctctat
     3901 cttttgcaaa gaagtcacca gattcacaga caggccccac tagatctcct gacctcgtgc
     3961 ccgggcgcaa aggatcagag aagacgtgat ggtaggcgtc atagagagaa ggacgaatga
     4021 gatcgttcat gcccgcatcc ccaatgaaaa atgttttctc cccctctttc acgtagagaa
     4081 ttttggacac gagtatgccg gcgttcccgg cgatgcttct gcctggatca aacatcaatt
     4141 ccaggtgatc gagtcctacg gattggatga gagttcgaac ataggtggca gggtcaggtg
     4201 cttcttcatt tgcataacga atgcccagcc cacctcccac atcgagatgc ttgagtttga
     4261 ttcccgcagc ttcaatttca gaaacgagcc tcactgcgat ctttcctgca tccgcaaagg
     4321 cagatagatc cgtgatctgt gatccaatat gaaagcctaa tcccgttggc tcaatccccg
     4381 gcaatgattg ggcaagttta taaattctca caacttcttt atggctgatg ccgaatttat
     4441 tttcttttaa cccggttgaa atgtatggat gcgttccggg gttcacatcc ggatttacgc
     4501 gcacactgat acgagctgtc tttcctgtaa tcgttgctat gcgagaaatt tcatgcagtt
     4561 ctgcttccga ttccacactg agaaacaaaa tcccttcagt gatcgcataa cggatctccg
     4621 cttctgtttt tcccacgcca gaaaacacta ttttctccgg ggaaatgtca gctttgcggc
     4681 atctaaacaa ttctccgccg ctgacaatgt ccgctccaca gccaaggcca gcgagtatgc
     4741 gcaaaatgtg aatattcgag catgctttta cggaataata gatgcctgcc cgcctgtttc
     4801 cgaaggcaga gccgagggcc tggcaacgtt ctaccagggc gctttc
//
LOCUS       DAOQJL010000281         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10379_length_4834_cov_4.869429, whole genome shotgun sequence.
ACCESSION   DAOQJL010000281 DAOQJL010000000
VERSION     DAOQJL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10379_length_4834_cov_4.869429"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLG78_12175"
     CDS             complement(<1..461)
                     /locus_tag="PLG78_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE23920.1"
                     /translation="MKPGTYSTPNSVSTTTGRLLARNRTWFYLEYLHIVFRSRALAKK
                     GLYSREAWAKSSADVLALYERRGGQLTVEGLDQLHKLNGPVVFVANHMSTAETQILPG
                     FIQPVRDVTFVVKDSLMKGPVFGPIMRAVDGIGVTQKDARGDFETVMKEGC"
     gene            complement(630..980)
                     /locus_tag="PLG78_12180"
     CDS             complement(630..980)
                     /locus_tag="PLG78_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23921.1"
                     /translation="MKLLKTISLILVTIYLSGCTSYLGKFAILSTKDVDMSKQYEKMK
                     EPVEGSAATHIIILFPTSLSTIPLFAEATIDAMTKSGADFLENAEIETSAFYIPFIYG
                     KFMATVKGTGYKLK"
     gene            complement(982..1320)
                     /locus_tag="PLG78_12185"
     CDS             complement(982..1320)
                     /locus_tag="PLG78_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23922.1"
                     /translation="MKLTALVAIGSALLLSACETYKMGTVSVLPAKTGTEYELVTTAA
                     MKAHNRPIIFGVTFGEEKNFMDVMNDLQASAGCALLKKVEMRQYAVTYLGFGSNTTSV
                     KGECYKVKGK"
     gene            complement(1549..2454)
                     /gene="xerD"
                     /locus_tag="PLG78_12190"
     CDS             complement(1549..2454)
                     /gene="xerD"
                     /locus_tag="PLG78_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNE23923.1"
                     /translation="MAKTVPNTQKLIKDFQDYLLVEKGLSENSILSYHYDLKKFHTYA
                     QSRKKDLLKIDTDDITNFLKDQKRKKISSRSLARSVAALRQFYRYLKEEKRVVRNPVD
                     KVQAPEVQRTLPDYLTLGEINQLFAAIKETDAFEMRDKAIFELLYSSGLRISEACTLR
                     MEDVDMENMFITVRGKGGRERLVPFGEHSKMILSRYLDKARHDILRERQSVYIFISKK
                     GDSLNRKSVWRLLKKYLERTDIRKVVTPHTFRHSFATHMIENNADLRSVQELLGHIDI
                     STTQVYTHLANRTLKEVHKKYHPRG"
     gene            complement(2625..4286)
                     /locus_tag="PLG78_12195"
     CDS             complement(2625..4286)
                     /locus_tag="PLG78_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE23924.1"
                     /translation="MEFDFHSLARRSHQARDSFRLGMILAAIVALLVQILFDLERRSG
                     AIAGVPIAAAVFAILLIVFRGVAKVRLLKPVSAAAFHILFVIHFASCFLFDAFYEDAV
                     HRRYSLLDADPDNVAYLFQAILPWTVVISLIVSIVFIYASAFFLHHQIRKPGLLAAAI
                     LIAGTVGAVRADRPALHAFVASDVKDYISGSPLGKPASQNLDVRDYQDASARSLPVSG
                     KYKRVILFVMESITVKELARDCADSSCFPQKYANITHSYKNYYAANQDSRTGILSLLY
                     SAFIPFPAYADRDVEQYEFIGKSKSFLDALHQSGYTTAFSASLSDRERVIMDMPWKKV
                     FRLSEEEAKGGGKFVCLHPYEFERSCEDRILFDRIMDFVSRDPRPFLMHEFVYGHSSQ
                     YNEIEKKTSSRYYSEFISDVISGLEARGLLDETLIIVTSDHGIRDRAAMTETESYHIP
                     LLFIQKNFRRTENSAFVSPIDFRRIFAAEERGEVIPSTRKILPIMGSTGSAMTGILHG
                     VTGLVLIKNRRFGARALETSCHDCPEASDVLPMLEQYRRAFRQIR"
     gene            4382..>4834
                     /locus_tag="PLG78_12200"
     CDS             4382..>4834
                     /locus_tag="PLG78_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNE23925.1"
                     /translation="MSDSTKKATPEKGNGKKKVEDPLKDIVSLAKRRGLVYPGSDIYG
                     GLANTFDYGPYGTELMRNLKDMWWRAFVREREDVVGLDSSILLHPEVWKASGHVDSFN
                     DPLVDCKQCRARFRADHIVEQLDPNGPKAMTLEQLVEWFEKTETQCPTC"
BASE COUNT         1148 a         1121 c         1247 g         1318 t
ORIGIN      
        1 tcgcatcctt ccttcatcac tgtttcaaaa tcccctcgcg catctttctg tgtgacgcca
       61 atgccgtcca ccgcgcgcat gattggaccg aacaccgggc ccttcatgag agaatctttc
      121 actacgaatg tgacgtcgcg tactggctgg ataaagcctg gaaggatctg agtttctgca
      181 gtgctcatgt gattggcgac gaatactacc ggtccattca gtttgtggag ttgatcgagg
      241 ccttctaccg tgagttgtcc gcctctgcgt tcatacagtg ccagaacatc tgctgagctt
      301 tttgcccacg cttctctgct gtagagtcct ttctttgcca gtgcccgcga acgaaacaca
      361 atgtgtagat attccaggta aaaccaggtg cggttgcgag ccagaagtcg cccggtcgta
      421 gtcgaaactg aatttggtgt agaataggtg cctggtttca tacatcttcc tgtatttaac
      481 gttgttagtt ttaatgtatg aatcggttta gccggaaagg ttgcaaaaaa gagaacgctt
      541 ttatttacga ttggagtctg cattacagac tatgccggga agaagagaga gccaataacg
      601 ttcaaccttg ctattggctc tcgttgagtt tatttgagtt tgtagcctgt tcctttcaca
      661 gtcgccatga atttgccgta gatgaaaggt atgtagaaag cggatgtctc aatttccgca
      721 ttttcgagga aatctgctcc cgattttgtc atcgcatcga tcgtcgcttc tgcaaatagc
      781 gggatagtgc tgagagaagt tgggaataag atgatgatgt gggttgcggc tgatccttcc
      841 accggttcct tcatcttttc gtattgcttg gacatatcca cgtctttggt ggaaagaatt
      901 gcgaatttgc ccaggtagga agtgcaacca gagagataga tggttaccag gataagcgag
      961 atcgttttta atagtttcat attatttacc ttttaccttg tagcattcac ctttgacgga
     1021 agtggtgttg ctcccgaatc caagatacgt caccgcatat tgtctcattt caaccttctt
     1081 gagaagggcg cagcccgcac tcgcttgcag gtcgttcatc acatccatga aattcttttc
     1141 ttcgccaaaa gtgacgccaa agataattgg cctgttgtgg gctttcatgg ccgccgtagt
     1201 aaccagttca tattcggttc ctgtttttgc gggaagcacg ctgacggttc ccattttata
     1261 tgtctcgcaa gcggagagca gcaatgctga tccgatcgcg actaacgctg taagcttcat
     1321 ttagtcctcg cctgcaggtc gtgctgattg acaaaaagag gcaagcaaaa atgccagcaa
     1381 tcgagccttt ctggagccat gtgttgttca ttaccaaaaa agaggcgttc attcggacgc
     1441 ctgagttgct aatactcctt tagtttttcg ccttgacagg cgtttggtgg cggggtactc
     1501 atttccgctt acagcggccc attccaggct caattttggc gcgcggtgtt atccgcgcgg
     1561 atggtatttc ttgtggactt ctttgagagt acgattggcc aggtgtgtat acacctgagt
     1621 ggtcgaaatg tcaatgtggc ccaggagttc ctgcacactt ctgaggtccg cattgttttc
     1681 aatcatatgt gtcgcaaagc tatgccggaa tgtatgagga gttacaactt tcctgatgtc
     1741 cgttcgttcc agatatttct tgagcaatct ccagactgat ttgcgattga gagagtctcc
     1801 cttcttgctg atgaatatgt aaacgctctg gcgttcgcga aggatgtcat gacgagcctt
     1861 gtccaggtaa cgcgacagaa tcattttgga atgctctcca aacggaacca gtctttcgcg
     1921 cccacctttg cctcgaactg tgataaacat gttttccatg tctacgtctt ccatgcgcag
     1981 cgtgcacgct tctgagatac gtagaccact tgaatacaga agttcgaaaa tcgccttatc
     2041 cctcatttca aatgcgtctg tttctttgat cgcggcaaat agctggttga tttcgcccag
     2101 tgtcaggtag tcaggaagcg ttctctgcac ttccggtgcc tgcactttgt cgactggatt
     2161 gcgcacgacg cgcttctctt ctttcaaata gcgatagaac tggcggagtg ctgctacgga
     2221 acgagccagg gatcggctgg atattttctt tctcttctga tctttgagga aattagtaat
     2281 gtcatcggtg tcaattttga gcaggtcctt tttgcgggac tgcgcgtagg tgtggaattt
     2341 cttgaggtcg tagtgatagg aaaggataga attttcagac agcccctttt ctacgagaag
     2401 gtagtcctga aaatctttga tcagtttttg tgtgtttggt acagtcttgg ccacgctagt
     2461 ctccctgata aattttcgga gaactggcca aagcgaaggc aacttttttg ccctttatgc
     2521 cgcatttagt cacatcgctt taattcttcg ttctgtctcc tggggccgga cataattcga
     2581 atgtactttt tcttttgatt ttagcaacaa aattcagccg gttttcagcg gatttgccga
     2641 aaggcccggc ggtattgttc gagcatgggt agtacgtccg aggcttccgg gcagtcgtgg
     2701 cacgatgttt cgagcgctct cgcaccgaat ctgcggttct tgatcagcac cagacctgtg
     2761 acgccgtgta gaataccagt catcgcactg ccagtggatc ccatgatagg aagaatcttg
     2821 cgcgtagatg gaatgacttc tccgcgttct tctgccgcga aaattctgcg aaagtcaatg
     2881 ggagaaacga aggctgagtt ttccgtgcgg cggaaatttt tttgaatgaa aagcaacgga
     2941 atgtgatagc tttcagtttc tgtcatggct gctcgatccc ggatgccgtg atctgatgta
     3001 acaatgatga gagtttcgtc taacagaccg cgggcttcga gtccagagat cacatctgag
     3061 atgaattcag aatagtatcg cgaagaggtt ttcttttcta tttcgttgta ctgactgctg
     3121 tgaccgtata caaattcgtg catcaggaaa gggcgaggat cacgcgaaac aaagtccatg
     3181 attcgatcga acaagattct atcttcgcag cttctttcaa attcgtacgg atgcaggcat
     3241 acaaatttgc cgccaccttt cgcttcctct tcactgagtc taaaaacttt cttccagggc
     3301 atgtccatga tcaccctttc gcgatcggag agagaggcgg agaaagcggt cgtgtatccg
     3361 gattgatgga gcgcatccag gaacgatttg cttttgccaa tgaattcgta ttgctctaca
     3421 tctcgatccg cgtacgccgg gaagggaata aaagcggaat agaggagact gagaattcct
     3481 gtgcgactgt cctgattggc cgcgtagtag ttcttgtagg agtgcgtaat gtttgcgtat
     3541 ttctgcggaa agcaggatga gtccgcacaa tcgcgggcga gctctttgac tgtgatgctt
     3601 tccatcacaa aaagaatcac tcttttgtac tttccggaca ctggcagaga gcgagcgctc
     3661 gcgtcctggt aatctcttac gtccaaattt tgtgaggctg gtttgcccag aggactgccg
     3721 gagatatagt ctttcacatc ggaggctaca aacgcgtgca gtgctggtct gtccgcgcgc
     3781 acggccccca cggttcccgc gatcaggatg gccgccgcaa gtaggccggg ctttcgtatt
     3841 tgatgatgca gaaagaaggc gctcgcatag atgaaaacga tagacactat aagagaaatt
     3901 accactgtcc agggcaggat tgcctggaac agataggcga cattgtcagg gtctgcgtcg
     3961 agaagagagt atcttctgtg gactgcgtct tcgtagaagg catcaaacaa gaagcagctt
     4021 gcaaaatgga tcacaaacag tatatggaaa gccgcagctg acacaggctt aagaagtctc
     4081 acttttgcca ctccgcgaaa aacaatgagc agaatggcga acaccgccgc ggcaatgggt
     4141 acgccggcga tggcgccgga tctgcgttcc aaatcaaaca gaatctgaac cagcagggcc
     4201 acgatggctg ccagaatcat tcccagtcga aacgaatcgc gggcctggtg tgatcgcctt
     4261 gcgagagagt gaaaatcgaa ctccacatct cccgatatct ggaagcgcgt cctgcggcaa
     4321 gcaaatggca ttctcagttg caaggctcgg tgcttctggc gatctggcgc cggagcttgt
     4381 gatgtctgat tcaaccaaaa aagcaacgcc tgagaagggc aacggaaaga agaaagtaga
     4441 agatcctttg aaagacatag tatcgctggc aaaacgccga ggactcgtct atcccggatc
     4501 agacatctat ggtggcctgg ccaatacgtt tgactatggt ccgtacggca cagagctcat
     4561 gcgcaatcta aaggatatgt ggtggagagc ttttgtgcga gagcgcgaag atgttgtggg
     4621 tctggatagt tcgattctgc ttcatccgga agtatggaag gcaagtggtc acgtcgattc
     4681 gttcaatgac cctctcgttg attgcaagca gtgtcgcgca cgctttcgcg cagatcatat
     4741 cgtagagcag ctggacccaa atggtccaaa agcgatgact ctcgaacagc tggtagagtg
     4801 gttcgaaaag actgaaacgc agtgccccac ttgc
//
LOCUS       DAOQJL010000282         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10501_length_4794_cov_4.904410, whole genome shotgun sequence.
ACCESSION   DAOQJL010000282 DAOQJL010000000
VERSION     DAOQJL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10501_length_4794_cov_4.904410"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLG78_12205"
     CDS             <1..1267
                     /locus_tag="PLG78_12205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE23926.1"
                     /translation="NVVIFTDTYLPKVDGVGISVDHFTRILGGRGHNFVICAPRYSDH
                     DPETISDHIHVIRFKNAPLPSYPDIKVVIPSHKKLTKAMTMFPPDLVHIQTPGLLGQY
                     GVLAAKMYGVPMIGTYHTLVSEQDTYISLYRLLKVDTLLNYFKSHKKIEKRLDKIERK
                     KKEKTLKSQLIYKLTLGLYQAGELIISPSHLIKKDLVEGGASIPIEVVSNGMDLDRFR
                     GEIKEPRKPPKFLHVGRISFEKNCEVVLKAFALILEKIPDATLDIIGDGPALTSIKIE
                     AKQIGVFDRCNFPGFVAHGSLHEIYPKYDLFLTASTMETQGLVVLEAMACGLPCVGVN
                     AYALPELIQHERNGFVVEPFDHIHMAERALQLLGDPELYKKFSAQSLAIAGEHELNSC
                     ADRLESVYHSVVEKHRKKPAGLFGGLLPG"
     gene            complement(1275..1964)
                     /locus_tag="PLG78_12210"
     CDS             complement(1275..1964)
                     /locus_tag="PLG78_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23927.1"
                     /translation="MNYFEIEKNLQVALDDGQKIEIISFRVDEAVEKQLLLTVEKILE
                     RYSRAHLKDALFSCALELATNATKANMKHVYFTERGLDIDKPEDYEKGMAGFKDVVLT
                     RPWLEAYAEKARSLGLHVQMIFTHTDDGLRIEIINNRPILAQDEERIREKFQKAMTYD
                     SLHEYFLDHADQTEGEGLGFALNILLLKGENLDATLFRIGREERGTIARIEVPFTDKF
                     VSVRPKEKPDA"
     gene            complement(2035..2772)
                     /locus_tag="PLG78_12215"
     CDS             complement(2035..2772)
                     /locus_tag="PLG78_12215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE23928.1"
                     /translation="MIRAFVLFFALITPAYAEGTLYVLCYHSFQGRKTDYDVAPEAFE
                     KQMQQVHDLGYRFVRTEDVLADRVNGAKNVLVSIDDGNKSVEAIYDRVLKKHDIKPLL
                     FIYPNIINRSNVPYAMTNDQLNRYLREGATLGAHGYYHLFLNEKQYNKDKKGFLKEIY
                     DSRDTLESRFGHRPEVFAYPFGVFSDLARDEIKKAGYKYSFTIVQGPVLLPLARNADP
                     FKLPRFLVTKTSWKNVLAVLERAVRNR"
     gene            complement(2775..3386)
                     /locus_tag="PLG78_12220"
     CDS             complement(2775..3386)
                     /locus_tag="PLG78_12220"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNE23929.1"
                     /translation="MRVFHEVAHPWHGIHPGEQAPELLTAYIEIVPLDTVKFELDKTT
                     GHLIIDRPQQYSSLCPTPYGFIPRTYCGDGIAALARAKNKKIEKGDGDPLDICVLAEA
                     TITRSDILLRARPIGGLRLIDRGEADDKIIAVMRGDALFDACTDIKHIPETLLDRLRH
                     YFLTYKQIPGAGPRAVEIAEIYGVETAHEVIRVAMNDYRVSYT"
     gene            3475..3897
                     /locus_tag="PLG78_12225"
     CDS             3475..3897
                     /locus_tag="PLG78_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE23930.1"
                     /translation="MYQKGKTFDQIAVGEKASFTKTITETDIYLYAGISGDFNPLHVD
                     EEYAKTTPFKGRIAHGGLAGSLLAPVLGMKLPGLGTMALEVTQKYRSPVFPGDTITCT
                     VEVTEKIARMKAVDMKILWTNQKGETIAKGNCRVLPPA"
     gene            3954..4739
                     /locus_tag="PLG78_12230"
     CDS             3954..4739
                     /locus_tag="PLG78_12230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE23931.1"
                     /translation="MTERKEVALITGASSGIGQDIARILAEKKIDLIITARRRDRLDA
                     LAKDLRTIHNIQVDVIESDLAKPDGAVQLYNQARAISKDITIVVNNAGFGKQNPVLDQ
                     SIEEIRSMIQVNMTSLTELTRLFAADMKAQGYGRILQLSSIGAFQPSPIYAVYSAAKS
                     YVLSFSYAMNHELKGSGVSITTMCPGITETEFHDVAGHEKTGFMKSFSMTSRKVAEIG
                     VRAMMKRKANVIPGFSNAINSWLMESMPRAVSTSIAASLMKKS"
BASE COUNT         1269 a         1191 c         1171 g         1163 t
ORIGIN      
        1 gaacgttgta atatttacag atacgtatct tcctaaagtg gacggcgttg gaatttcagt
       61 ggaccacttc acccgaatcc tgggcggacg aggacacaat tttgtaatat gtgctccgcg
      121 atattcagac cacgatccag aaaccatcag cgatcacatt cacgtcattc gctttaagaa
      181 cgcaccactc ccttcgtatc ctgacatcaa agtggtgatc ccttcgcata aaaaacttac
      241 gaaggccatg accatgttcc caccggatct ggttcacatc cagactccgg gcctgctggg
      301 ccagtacgga gtgctagcgg ccaaaatgta tggagtgcca atgataggca cgtatcatac
      361 tcttgtgtca gaacaggaca cgtacatttc gctctatcga cttttgaaag tggatactct
      421 tctgaactat ttcaagtcgc acaagaaaat tgaaaagcgt ctggataaaa ttgaaagaaa
      481 gaagaaagaa aaaacactca aaagccagct gatctacaag ctcactctcg gactctatca
      541 ggcgggcgaa ctcatcatca gcccaagtca tttgatcaag aaggatcttg tagaaggagg
      601 agcttctatc ccaatcgaag tggtctccaa cggaatggat ctcgatcgct ttcgcggcga
      661 aatcaaagag ccgcgcaaac caccaaagtt tctgcatgtg ggtcgcattt cttttgagaa
      721 gaactgtgaa gtggtactaa aagcattcgc cttgattctg gagaaaatcc cggatgccac
      781 actcgacatc atcggagatg gcccggcgct tacatccatc aaaatcgaag ccaagcaaat
      841 cggagtgttc gatcgctgca atttcccagg atttgtggcg cacgggtctt tgcatgagat
      901 ttatccaaaa tacgatctct tcctgacggc aagcacaatg gaaacgcaag ggcttgtagt
      961 cctcgaagcg atggcatgtg gactaccgtg cgtaggagtc aatgcatacg ctcttcccga
     1021 actcattcag catgaaagaa acggcttcgt ggtagaaccg tttgatcaca ttcacatggc
     1081 ggaacgcgca ttgcaactgt taggcgatcc tgaactctac aagaagttct cagctcaaag
     1141 cctggccatc gccggagagc atgagctcaa ctcctgcgca gatcgcctgg agagtgttta
     1201 ccacagtgta gtagaaaaac ataggaaaaa gcccgcagga ctctttggcg gactgctccc
     1261 gggttgaggt gtgatcaagc gtcgggcttt tcttttggcc ggacgcttac gaacttatct
     1321 gtaaaaggaa cttcgatgcg ggcaatggtc ccgcgttctt cccgaccaat tcgaaacaat
     1381 gttgcatcca ggttttcacc tttgaggagc aaaatgttta gtgcaaatcc aagtccttcc
     1441 ccttccgtct ggtctgcatg atccagaaag tattcatgga gggaatcgta cgtcatagcc
     1501 ttctggaact tttctcggat gcgctcttca tcttgcgcga gaataggacg attgtttatg
     1561 atttcaatgc ggagtccatc atctgtgtga gtgaaaatca tttgaacatg caaaccgagt
     1621 gagcgcgcct tctctgcata tgcttcgagc catgggcgag tcagcaccac gtctttgaat
     1681 ccggccattc ccttttcgta gtcttccggt ttgtctatat ccaggccccg ctccgtaaaa
     1741 tacacatgct tcatgtttgc tttggttgca ttggttgcca gttccagagc gcaggagaag
     1801 agagcatctt tcaaatgagc gcggctgtat ctctccagga tcttttcgac cgtcaggagc
     1861 agctgttttt ccactgcctc gtccacgcgg aaggaaatga tctctatctt ctggccgtca
     1921 tcgagcgcta cttgaagatt cttttctatt tcaaaataat tcatggtcga ttaacggttc
     1981 ggacgagggc gacttgcaac gtaccgggct attccagggt tttcaagcaa aaagctaacg
     2041 attacggact gcgcgttcca gaactgctaa aacgttcttc cagctggttt tggtgaccag
     2101 gaaccgaggc aatttgaaag gatccgcgtt tcttgccagc ggaagaagaa ccggaccctg
     2161 gacgattgta aacgaatact tatatccggc ctttttgatt tcatctcggg ccagatcaga
     2221 gaaaactccg aacggataag caaatacttc cggcctgtgt ccgaatctgg actcgagtgt
     2281 gtctcttgaa tcgtaaattt cctttaagaa tcctttcttg tccttgttgt actgcttctc
     2341 attgagaaag agatgatagt aaccatgcgc acccagggtc gcgccttccc gcaaataccg
     2401 gttgagctga tcgttagtca tggcataggg aacgttcgaa cgattgatga tgttcgggta
     2461 aatgaaaagc aaaggcttga tgtcgtgctt ctttaagaca cgatcataga tcgcttccac
     2521 tgatttattt ccatcgtcta tgctcaccag aacgttcttt gcgccgttca ctctgtctgc
     2581 cagaacgtct tctgttcgca cgaagcgata acccagatcg tgaacctgtt gcatctgctt
     2641 ttcaaatgcc tcaggcgcta cgtcgtagtc tgtctttctt ccttgaaacg aatggtagca
     2701 aagcacatac aatgttcctt ctgcgtatgc aggagtgatc aaagcaaaaa aaagaacgaa
     2761 cgcacgaatc atacttacgt atagctcacc ctgtaatcgt tcatggcaac gcgaatcacc
     2821 tcatgagcag tttccacgcc atagatctcc gcaatctcga cagcgcgagg gccggcgcca
     2881 ggaatctgtt tgtacgtaag gaaatagtga cgaagtcgat cgagcagagt ctctggaatg
     2941 tgtttgatgt ccgtgcaagc atcgaataga gcatctccgc gcatcaccgc gatgatcttg
     3001 tcatctgctt ccccccggtc gatcaggcga agcccgccaa tgggacgggc gcgcagcaga
     3061 atatcgcttc gcgtgatggt tgcttccgcc agaacacaaa tgtccagtgg gtctccatcc
     3121 cctttttcaa tcttcttgtt cttcgcacga gcaagggcag caattccgtc tccgcaataa
     3181 gtgcgcggga taaatccata cggagtggga cagagactgg agtactgttg agggcgatcg
     3241 atgatcaaat gaccggtggt cttatcgagt tcgaacttca ctgtgtccag cgggacaatt
     3301 tcaatgtacg cagtgagaag ttctggcgcc tgttctccgg gatgaatgcc atgccaggga
     3361 tgcgcaactt cgtgaaatac tctcatggcg cagatttcaa cgggcaccag ggagggcaaa
     3421 tgattctgga ggcttttttc ttgccggcca aacgtgcctg aaaggagcta cgacatgtac
     3481 caaaaaggga agaccttcga tcaaatcgcc gtcggagaaa aggcaagctt cacgaaaact
     3541 atcacggaaa cggacattta tctatatgcg ggaatcagcg gcgatttcaa cccactgcac
     3601 gtggacgaag aatacgcgaa gaccacaccc ttcaaaggca gaatcgcaca cggggggctg
     3661 gccggatctc tcctggcgcc cgtccttggt atgaagttac ccgggctagg cacgatggct
     3721 ctggaagtaa ctcaaaagta tcgtagcccg gttttccctg gagataccat cacctgtacc
     3781 gtggaagtga ctgaaaaaat cgcgagaatg aaggctgtgg acatgaaaat cctatggacc
     3841 aaccagaaag gagagacgat tgcaaaggga aactgcagag ttctaccgcc tgcctgagga
     3901 gacgcagagc ggtttagcgc ttgcgctttt ctgcaagcac agttcgatgc ggtatgacgg
     3961 aaagaaaaga agttgctctc atcacaggcg catcgagcgg aatcggccaa gacatagcac
     4021 gcatcctcgc tgaaaagaaa attgatctga tcatcactgc acgcaggcgt gatcgcctgg
     4081 atgctctggc caaagacctc agaaccatac acaacattca agtagacgtc atcgagtctg
     4141 atcttgcaaa accagacgga gccgttcaac tctacaatca ggctcgtgcc atttctaaag
     4201 atattacaat agttgtaaat aatgcaggat tcggaaaaca gaatcccgtc ctcgatcagt
     4261 ccatagagga aattcgctct atgatccagg tgaacatgac ttccttgacg gagctcacac
     4321 gactattcgc tgctgacatg aaggcccagg gctatgggcg aattctccag ctcagctcca
     4381 ttggagcgtt ccaaccttcg cccatctatg cagtctactc ggcagcaaaa tcgtatgttc
     4441 tttctttttc gtatgcgatg aatcacgaac tcaagggaag cggtgttagc attactacaa
     4501 tgtgtcctgg gatcacagaa actgaattcc acgatgtagc cggtcacgaa aagaccggat
     4561 tcatgaaatc gttctcgatg acttcgcgca aagtggcaga gatcggcgtt cgcgcgatga
     4621 tgaagcggaa ggccaatgtg attcctggtt tctccaacgc aatcaacagc tggctcatgg
     4681 aatctatgcc gcgcgccgtt tctaccagca ttgcggcctc cctgatgaag aaaagctaac
     4741 gcttggattt gaaaaaggga gtccggcgga agggcaaatc aacgagcgca aaga
//
LOCUS       DAOQJL010000283         4764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10604_length_4764_cov_4.459121, whole genome shotgun sequence.
ACCESSION   DAOQJL010000283 DAOQJL010000000
VERSION     DAOQJL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10604_length_4764_cov_4.459121"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLG78_12235"
     CDS             complement(<1..615)
                     /locus_tag="PLG78_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23932.1"
                     /translation="MKKGAITWTVCILLLQPLFSETIPPEAAVENLTTNWKGDDTNPL
                     PSPDGSFLVFQSDRPGSLEDFNFWFSYNKNHSDRTGASSWTIPVPLRFPLTGEASDTM
                     KILRPQGSVGVEPGLFSVNSDGFDGGGSILWRNDSPVEMYFTSDAKARGEGYDGLNIY
                     YTRFRDDRWSKPIQIDTLCSNFDDRMPWISKDGLRIVFSSNRPGG"
     gene            complement(554..1687)
                     /locus_tag="PLG78_12240"
     CDS             complement(554..1687)
                     /locus_tag="PLG78_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23933.1"
                     /translation="MRTIDLIRKIYIDESVADSPLARNVRLRALPGVEIETTSQSAEI
                     LNHFRKNGNIEDKHTLLLYPFPGKFMSFCPGSDGMVCCNYFVINFGYGCLYDCHYCFL
                     QNYLNNPLLTMFANTNDLFAEIDKKIHGKKFHFRIGTGETTDSLALEPLTGMAEILVN
                     HFAELPNATLELKTKSSNVDSLLGLDHRGHTVISWSVNPPGIVSEVETGTATLEERLA
                     AARKAEAAGYRLAFHLDPVIHFEGWEKQYHDLIDLIFSTVRADSVAWLSLGSFRYLPG
                     LKTVIQGRFPEDTLTREEMVQGPDGKYRYFKTIREEMYRSIRAKVQSVDPKLFLYLCM
                     ETKRMWEDVFQFVPESAKNLDAGFEERRNYMDSLHPPAAAPVL"
     gene            complement(1701..2162)
                     /locus_tag="PLG78_12245"
     CDS             complement(1701..2162)
                     /locus_tag="PLG78_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE23934.1"
                     /translation="MDHRFDVETFWEFSGGEGMNFSRDHVIRFPGKTEIHGSSAPAFA
                     GNAANLNPEELFVSAISSCQMLTYLFLCFKNKLQIQSYEDHAEGELVRESAGKFWMKT
                     VTLKPIVKFRGADTQQVRALAIRLIHEAHVNCFIAQSVKTTVNIEPSLLFM"
     gene            complement(2206..3219)
                     /locus_tag="PLG78_12250"
     CDS             complement(2206..3219)
                     /locus_tag="PLG78_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE23935.1"
                     /translation="MNSTVLSPDLEIQTHPDYNTSHHTENGFRNPWKMEEPKPGPLDA
                     LGWMVSFAFKKREDLPLFSVPMDPSFVANHPDLRSAHWIGHSTVLLKLGGKYVLLDPV
                     FANRASPVPFAGPRREVDLPIEIEDLPGVDVVVLSHNHYDHLDTDAIARLAGKFDPLF
                     IVPLRNGDVMRSAGARRVIELDWRQSVSAEGMTFHCLPARHFSNRGLTDRNEMLWASF
                     LIEHDGWKAYFAGDTGYAEHFSEIGRMFPGIDLALIPIGAYKPRWFMREVHVDPEEAM
                     QAFVDLGASEFFAIHWGTFILAEEALHEPAERMEQLILKKKMDRSRFHILMAGQSVGL
                     DRF"
     gene            complement(3229..>4764)
                     /locus_tag="PLG78_12255"
     CDS             complement(3229..>4764)
                     /locus_tag="PLG78_12255"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNE23936.1"
                     /translation="KSYQFMLVDGGNGTMNHVYIDNLIDAVILLVEKNATGRAYNVTD
                     GGRTTFREYFQRLAREAGRGRLPSLPYNVARAVAWAAALPYKLFGRRPEVSADSIPFV
                     TRPHPVSIEAITALGFVPRVTLDEGIERTMEWLRSVNYEELHPFRPQTEEKPSRYRYA
                     ALIGVILLAYLLFFPVKIDPGPYVAQADVGYTGPFAPNQKLADIKMLPISAHGPEHVV
                     VDSGWIYAAVDSGAILRMRADGSSLEEFVQTGGRPLGFAFDAKKNLIIADAVKGLLLV
                     TAKKEIHVITDQVDGKPLQFTDAVVVSKSGKIYFTDASMRFSARGGTFEASILDTLEH
                     SCTGRLIEVEAGRAKVFSSGICFANGLALDSKEESVFVAETGEMRILRYWISGARKGV
                     KEVFLDKLPGYPDNLMRGSSGKIWVGLPRPRSKFADLAAAHPILRKITLRLPRFLWPV
                     PKPYGHVFAFDESGRIVADLQDPAGQYPDTTGVTETADRLYIQSLHAEGLGYVLAAGR
                     TAQ"
BASE COUNT         1214 a         1226 c         1163 g         1161 t
ORIGIN      
        1 cccgcccggc cggttcgaag aaaacacaat gcgaaggccg tctttagaaa tccagggcat
       61 ccgatcgtca aaattagaac agagagtatc tatctggatt ggtttggacc agcgatcgtc
      121 gcgaaagcga gtgtaataaa tattgagccc gtcgtaacct tccccgcgcg ctttcgcatc
      181 cgacgtgaaa tacatttcaa caggactgtc gttgcgccag agaatcgagc cgccgccatc
      241 aaagccgtcg gagttcacac tgaagagtcc tggctctact cccacacttc cctgaggacg
      301 aaggatcttc atcgtatcag acgcttcgcc ggttagagga aagcgaagag gcacaggaat
      361 cgtccacgag gaagcgcccg tgcgatccga atgattctta ttataggaga accaaaagtt
      421 gaaatcttcc agggagcccg gacgatcgga ctgaaacaca aggaagcttc cgtccggcga
      481 tggaagcgga ttggtatcgt ctcccttcca gttggtagtt agattttcaa cggccgcctc
      541 tggcgggatc gtttcagaga acaggggctg cagcaggagg atgcagactg tccatgtaat
      601 tgcgcctttc ttcaaatcca gcatccaggt tcttcgcaga ttcgggcacg aattggaaca
      661 catcctccca catccgcttc gtttccatgc aaagatacag gaaaagcttc gggtccacag
      721 attggacctt cgcgcggatg gatcggtaca tttcttctcg tatcgtctta aaataacggt
      781 actttccgtc cggtccttga accatttcct cgcgagtcag cgtgtcttct ggaaaacgtc
      841 cctgaattac ggtctttagg ccgggtaaat agcgaaaact gcccagggaa agccaggcaa
      901 cactgtctgc cctcactgtg gagaagatta gatcgatcag gtcgtggtac tgtttttccc
      961 agccttcaaa atgaatcaca ggatccagat gaaatgcaag tctatatccg gcagcctccg
     1021 ccttcctggc cgcggccaga cgttcttcca gggtggctgt cccggtttcc acttccgaaa
     1081 cgattccagg cggattgaca gaccaggaga tcactgtgtg gccacgatga tcgagcccga
     1141 gcaagctatc cacgtttgag gattttgttt ttagttccag agtggcgttc ggaagttccg
     1201 caaaatgatt caccagtatt tccgccatcc ctgtgagcgg ttcgagcgca agagaatccg
     1261 tggtttctcc ggtgccaatg cggaagtgaa acttttttcc atgaattttt ttatcaattt
     1321 ccgcaaacaa atcattcgta tttgcaaaca tggtcaaaag aggattgttc aggtaatttt
     1381 gaagaaaaca atagtggcag tcatagagac agccatatcc aaaattgatc acaaagtagt
     1441 tacagcagac catgccgtcc gagccagggc agaaggacat gaacttacct ggaaaaggat
     1501 agagcaacag agtgtgtttg tcttctatgt ttccgttttt tctgaagtga tttagaatct
     1561 ccgctgactg actggttgtt tcaatttcca ctcctggaag agcgcgaaga cgcacgttgc
     1621 gcgcgagagg agaatctgca acggattcat ctatgtaaat tttcctaatg agatcaatgg
     1681 ttcgcataaa cgaccggcgc tcacataaac aaaagactgg gttcaatgtt cacggttgtc
     1741 ttcacagatt gcgcgatgaa gcaatttaca tgcgcttcat gaatgagcct gatggcaagt
     1801 gcgcgcacct gctgggtgtc cgcgccacga aatttcacga ttggcttgag tgtaacggtc
     1861 ttcatccaga attttccggc agattcgcgc accagttctc cctcggcatg atcttcgtag
     1921 gattgaattt ggagtttgtt cttaaaacac aagaagagat acgtgagcat ctggcaggaa
     1981 gagatggcag aaacaaatag ctcttcaggg ttcaggttgg ccgcgtttcc cgcaaatgca
     2041 ggcgcggagg atccgtgaat ttccgttttc ccgggaaaac ggatcacatg atcccggctg
     2101 aaattcattc cctctccgcc tgagaactcc cagaatgttt ctacatcaaa gcgatggtcc
     2161 ataggtcagt ttatctccgg gttaggcgag cgcaaccgat ttgcactaga atcgatccag
     2221 accgacagat tgacctgcca tgagtatgtg aaatcgcgac cgatccattt tcttttttaa
     2281 aatcagctgc tccatgcgct ctgctggctc atgcagagct tcctctgcca ggataaacgt
     2341 tccccagtga atggcaaaaa attcagacgc acccagatcc acaaaggcct gcattgcttc
     2401 ctctggatcc acatgcacct cccgcataaa ccatcttggc ttgtacgcgc cgattggaat
     2461 gagagccaga tcaatgccag ggaacattct gcctatttcg gagaaatgtt ccgcataacc
     2521 ggtgtcaccc gcaaagtagg ctttccatcc atcgtgctca atgaggaagc tggcccacag
     2581 catttcattc ctatccgtaa gacctctgtt cgaaaaatgt ctggccggca agcaatgaaa
     2641 cgtcatgccc tccgcactga cactctgcct ccagtccagt tctatcacgc gacgagctcc
     2701 tgctgatcgc ataacgtctc catttctaag tggcactatg aataaaggat caaacttgcc
     2761 cgccagacga gcgatcgcat ccgtatccag gtgatcgtag tgattgtgag aaagaaccac
     2821 aacatccact ccggggaggt cttcgatttc aatgggcaaa tccacttctc ttctgggacc
     2881 tgcaaatggg acaggactgg cacgatttgc aaaaactgga tcgagtagaa catatttgcc
     2941 gccaagcttg agcaacacag tagaatgccc aatccagtga gcagatctga gatctggatg
     3001 attggctaca aaggagggat ccatgggaac agagaaaaga ggtagatcct ctcgcttttt
     3061 gaatgcgaaa gaaaccatcc atcccagagc atcgagtggg cccggctttg gttcttccat
     3121 tttccacgga ttgcgaaacc cgttttcagt gtggtgactt gtattgtagt caggatgcgt
     3181 ttggatttca agatcaggag acagaacggt ggaattcact ccaacagatt actgcgcagt
     3241 cctgcctgcg gcgagcacat atccaaggcc ttcggcatga agactctgaa tgtagaggcg
     3301 atctgctgtc tctgttaccc ctgtggtatc tggatactgg cctgcgggat cctgaagatc
     3361 cgcgacaatg cgtccggatt catcaaatgc gaatacatga ccgtacggtt ttggaacagg
     3421 ccagagaaaa cgtgggagcc tcagtgtgat cttacgcagg attggatgag cagccgcaag
     3481 atctgcaaat ttactgcgcg gcctcggaag acccacccag atcttaccgg aactgcctcg
     3541 cattagattg tccgggtagc ccggaagttt atccaggaac acttctttta caccttttcg
     3601 cgcaccagaa atccagtatc gaaggatgcg catctcaccc gtttctgcta caaatacact
     3661 ctcctctttt gaatcgagcg caagaccatt ggcaaaacaa attccagaag aaaagacctt
     3721 cgcgcgacca gcctccactt cgatgagacg accggtacag gaatgctcca gtgtatcgag
     3781 aatgcttgcc tcaaacgttc cgccgcgagc ggagaagcgc atgcttgcat ctgtgaagta
     3841 tatttttcct gattttgaaa ctaccactgc atccgtaaat tgaagcggct ttccatccac
     3901 ctggtctgtg atcacatgaa tttctttttt cgcagtgacc aggagcagtc ctttcactgc
     3961 atccgcgatg atcaggttct tctttgcatc gaatgcaaat ccaagaggac gtcctcctgt
     4021 ttgtacaaat tcttccagac tcgatccatc cgcccgcatg cgaaggatcg cacctgaatc
     4081 cactgcggca taaatccagc cagagtccac aaccacatgt tcggggccat gagcgctgat
     4141 gggtagcatt ttgatatctg ccagtttctg gttgggcgca aagggtccag tgtaccccac
     4201 atccgcctgg gctacatagg ggcctggatc aattttgact ggaaaaaaaa gcaagtaagc
     4261 aagaaggatc accccgatca gtgccgcata acggtatcga gaaggtttct cctctgtttg
     4321 cggtcgaaat ggatggagct cttcgtagtt cacgcttcta agccattcca tggtcctttc
     4381 gattccttca tccagggtca cgcgtggaac aaatccaagt gcagtaatgg cttctataga
     4441 gacaggatgg ggccttgtaa caaagggaat tgaatcagcg gacacctcgg gcctgcgccc
     4501 aaacagtttg tacgggagag cggccgccca tgcaacggct ctagcgacat tgtaaggaag
     4561 agacggaagc cgtccgcgac ctgcttctcg ggccagtctt tgaaagtatt cccgaaacgt
     4621 ggttctgcct ccgtccgtaa cgttatacgc ccttcctgtg gcgttctttt ccacaagcaa
     4681 aatgaccgcg tctatcaggt tgtctatgta gacgtgattc atggtgccgt tcccaccatc
     4741 cacaagcata aactggtaag attt
//
LOCUS       DAOQJL010000284         4750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10649_length_4750_cov_4.264963, whole genome shotgun sequence.
ACCESSION   DAOQJL010000284 DAOQJL010000000
VERSION     DAOQJL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10649_length_4750_cov_4.264963"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLG78_12260"
     CDS             <1..1102
                     /locus_tag="PLG78_12260"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23937.1"
                     /translation="GDHPTHTVFYEGTGSARTPAGNAWMPTVRFSGFQKQIKPGDFLL
                     NDGGIVGVIAGFDESTNTGYAIPAAIIEQFLSSQVFDTQRNSVALQNQAGPEPIPSRV
                     PPTIVRSSGLGGATASEPAYLRYMGLVTERPAFLVTRTAVQRTEERAVMQGDVILSTP
                     GGRPSSDGKIKDEYYGDLPLPLAIVLKNGRFTDENAVSIQVLRDRVERMIRLPVRNAS
                     EDLSVVPEKLFHSSYLITGGLVLLELTGERVRAMDDSTGRFRYLRSRYWGGDNMIGER
                     IIFVDRVLPVKQNEGQRPQRAVLTSVNGIGVRNLLHAHSIIREARRKGEDVVFTFDGN
                     EIAVFSAAALAQADEQVRNSYGIPFLYGESLE"
     gene            1086..1553
                     /gene="smpB"
                     /locus_tag="PLG78_12265"
     CDS             1086..1553
                     /gene="smpB"
                     /locus_tag="PLG78_12265"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNE23938.1"
                     /translation="MSPSNKKPPPSFENKQARRKYEVTETYEAGIALTGTEVKSLRHG
                     KCEILDAHIIIKEGELFVLNLRIEPYEHGGSYNHAEKRTRKLLMHRTEISKIGAKIRE
                     KQMTCIPLKVYFNEKGKVKVLIGLGKGKKMADRREDEKKAVAKREMERAIRGR"
     gene            1555..2880
                     /gene="der"
                     /locus_tag="PLG78_12270"
     CDS             1555..2880
                     /gene="der"
                     /locus_tag="PLG78_12270"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNE23939.1"
                     /translation="MSANKKLPLIAIAGKRNAGKSTLLNIMFGSKRAITDATPGLTRD
                     ILQVEIKRGKHRFLLCDTPGLDLDGSEPLDANILSRTREFLEGVDLILFVMEPPGPTP
                     FDLSFLDFFRKQRDLSRILFVLNKIDNPKQDSAEAEFYQMGFPELICISALGRKNLGT
                     LYDQIEKRLPAPGSTVASAGPPPDLTLCIVGKPNAGKSSLLNRLSGQELALVSDIPGT
                     TRDSIDSLFTYYGKTIRLIDTAGLKKTGKLKDSVEFYSMTRTKRAIAEAEIVVHVLDA
                     TLGVTDYDKKIVSLIEENGKPVVFAVNKWDAVEEKNQKAFMDRMYFMFPHAQSYPAIF
                     ISAKTGTRIGKVLEEAMKLKERLSFRVGTAQLNQWLKEWNRRLTNTQKTAKIYYGTQA
                     GTAPPQFIFFVNKKDHFRKDALLYFENQIRKDCDLHGIPITIQLREKES"
     gene            2886..3527
                     /gene="plsY"
                     /locus_tag="PLG78_12275"
     CDS             2886..3527
                     /gene="plsY"
                     /locus_tag="PLG78_12275"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNE23940.1"
                     /translation="MQVLLIPFSFLCGSIPFAVIVGRMRGIDVMTQGSCNPGASNVYR
                     LAGPIPGILVFLLDVAKGALPALAGMYLAVHDLSGNATEPNPVLQIWLELGAGFSAAF
                     GHIFSPFLKFKGGKGVATFLGVFLVIFPAGILTACALAAVAILITRYFSVGSILGAIV
                     LPVSYFIFVDPVWAPQNVPVLCLTILASVVILIRHRENISRLIKGTEHKTRHV"
     gene            3520..4101
                     /locus_tag="PLG78_12280"
     CDS             3520..4101
                     /locus_tag="PLG78_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23941.1"
                     /translation="MFEHLQEISERASIESMTRTAEQFLDNHPIARARDLYRWLWEGE
                     FGPGTRHQPTRLDELTQMIRTARMHKSAGSQPMWEPLGLTCRFVHVNLIPYSDAGCPL
                     KRILTLGERTRDYRADQLRFKMQWAFMKTQIIHGMRITVEEMVSFENDIAFHMTPEIS
                     FTDEYLAEYGSGYMVVPYDLFFETYPEYAEHES"
     gene            4101..>4750
                     /locus_tag="PLG78_12285"
     CDS             4101..>4750
                     /locus_tag="PLG78_12285"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNE23942.1"
                     /translation="MRIPTGVTFEDGKLYYVDQTKLPGTFEKKECTTLEEAEEAIRQL
                     RVRGAPAIATFASFAAALEMDRKRHLAHPQWKVEAEQVIDRLLATRPTAVNLSHAMKR
                     LRERLAAGDRSLLSEAAAIQEEDKQLCDALGKSGSTLIPAGARVITHCNTGALATAGI
                     GTAIGSFYTAAAEGKDITVYACETRPLLQGARLTSWELDRAGIRTILITDSMAAFI"
BASE COUNT         1263 a         1167 c         1229 g         1091 t
ORIGIN      
        1 gggagatcat ccgactcata ctgtgttcta tgaagggaca ggaagtgcca ggactccagc
       61 aggcaatgcg tggatgccca cagtcaggtt cagtggattt caaaagcaga tcaagccagg
      121 tgacttttta ctcaatgatg gcggaatcgt cggagtgatt gcaggatttg atgaatcaac
      181 aaacaccggt tatgcgattc ccgctgcaat catcgaacag tttctgagct ctcaggtgtt
      241 tgatacgcaa cgaaattcag tggcgcttca aaatcaggca gggccagaac cgattccgtc
      301 tagagtacct cctacaatcg tccggtccag tggcctcggt ggcgcaaccg cgtctgagcc
      361 tgcgtatctt cgttacatgg gcctggtaac agaaagacct gcttttctgg taacacgcac
      421 agcggtacag cgaactgaag aacgcgcggt gatgcaagga gacgtgattc tttctacgcc
      481 aggtggaagg ccttcctcag acgggaagat caaagacgaa tactacggtg atttgccact
      541 tcctctggcg atcgtgctga aaaacggccg atttacggat gaaaacgctg tcagcattca
      601 ggttcttcgc gatcgggtag agcgcatgat tcgcctacct gtgcgaaacg catccgaaga
      661 tctgtctgtt gttcccgaga agctatttca ttcttcgtat ttgattaccg gtggactggt
      721 gctcctggaa ctcacgggag agcgggttcg tgccatggat gactctacgg gccgatttcg
      781 ctacctgaga tcgcgctact ggggcgggga caacatgata ggagaacgaa tcatattcgt
      841 ggaccgggtt ctgcccgtga aacagaatga gggccagagg ccgcagaggg cagtgctgac
      901 gtctgtcaac ggaatcgggg tcagaaacct gctccatgct cattcgatca tccgggaagc
      961 gcgccggaaa ggcgaagatg ttgttttcac ctttgacggg aatgaaatag ctgtatttag
     1021 cgcagctgct ctggctcagg cagacgagca ggttcgcaat tcatacggta taccattttt
     1081 gtacggtgag tccctcgaat aaaaaaccgc ctccctcttt cgagaacaag caggcacgcc
     1141 gcaaatacga agtcacggag acatacgagg cagggatagc tttaaccggg acagaagtga
     1201 agagtcttcg tcacggaaag tgcgaaatcc tggacgcgca catcatcatc aaagaagggg
     1261 aactttttgt tctgaacctt cgaattgagc cttatgagca cggcggatcc tacaatcacg
     1321 cagagaaacg tacgcgcaaa ctgctgatgc atcgaactga gatctcaaag attggcgcaa
     1381 agatcagaga gaagcagatg acatgcattc ctttgaaagt ctacttcaat gaaaaaggca
     1441 aagtgaaagt tctcatcggt ctgggcaaag gaaagaaaat ggccgataga cgcgaagatg
     1501 aaaagaaggc agttgccaaa agagaaatgg agcgcgccat aagaggccgt tgatatgagc
     1561 gcgaacaaga aactccctct catcgccata gctggaaaac ggaacgcggg aaaatctact
     1621 ctactcaata tcatgttcgg atccaagaga gcgatcacgg acgcaactcc tggcctcacg
     1681 cgtgacatct tacaggtaga aatcaaaaga gggaagcatc gcttcctttt gtgcgacact
     1741 ccagggcttg acctggacgg cagcgaaccg ctcgacgcaa acatactctc gaggacccga
     1801 gaatttctag aaggagtgga ccttatcctc ttcgtgatgg aaccaccggg tcccactcca
     1861 tttgatcttt catttctgga tttttttaga aagcagcgtg atctatccag aattctattt
     1921 gttctcaaca agatagacaa tccaaaacag gactcagcag aagctgagtt ttaccagatg
     1981 ggtttcccag agctcatttg catttccgcc ctcggaagaa agaatcttgg gactctgtat
     2041 gatcagattg aaaagagact gcccgcaccg ggaagtacag tcgcatctgc cggcccgcct
     2101 ccagacctga cactttgcat tgttggaaaa ccaaacgcgg gcaaatcgtc tttgctcaat
     2161 cgcctttctg gccaggaact ggcacttgtt tcagacattc cgggaaccac gcgcgacagc
     2221 atagactctc tgtttacgta ctacggaaaa acaattcgcc tcattgatac tgcaggtcta
     2281 aagaagacag gcaagctaaa ggattccgtg gaattctatt ccatgactcg cacaaagcga
     2341 gcgattgcag aggcggaaat cgtagtgcat gtgctcgatg caacgctcgg agttacggac
     2401 tacgacaaga agatcgtaag cctgattgaa gaaaatggaa aaccggttgt ttttgctgtg
     2461 aataaatggg acgctgtgga agagaaaaac cagaaggcgt tcatggacag aatgtatttc
     2521 atgtttcccc atgcccaatc ctaccccgcg atattcatat ctgcgaaaac aggcacgagg
     2581 attggaaagg ttctagaaga agccatgaaa ctcaaggaac gcctgtcctt tcgcgtgggc
     2641 acagcacagc tcaatcagtg gctaaaggaa tggaaccgtc gcctgaccaa cacccagaag
     2701 actgcaaaaa tctattatgg aacgcaggcg ggaacggccc ctcctcaatt catcttcttc
     2761 gtaaataaga aggatcattt tcgtaaagac gcacttttgt atttcgagaa ccagattcga
     2821 aaagactgtg atctccatgg cattccgatc acgattcagc tcagggagaa agaaagttga
     2881 tctgggtgca ggttctactc attccgtttt cgtttctgtg cggctccatt ccttttgcag
     2941 tgatcgtggg ccgcatgcgc gggatagatg taatgacgca gggatcttgc aatccaggag
     3001 cgtctaacgt ctacagactg gcaggtccca tccctggaat tctagtgttc ttgcttgatg
     3061 tggccaaggg tgctcttcct gcgcttgctg gaatgtatct ggctgtccac gatctgagtg
     3121 gaaatgcaac cgaaccaaat cctgttttgc aaatatggct ggaactgggc gctggatttt
     3181 ctgccgcgtt tggccacata ttttcacctt tcttaaagtt caaaggcggc aaaggagtgg
     3241 ccacctttct tggagtgttt ctggtcatct ttcccgcagg aattctaacc gcatgcgctc
     3301 tggcggccgt ggccatcctg atcacgcgct atttttctgt aggcagtatt ctcggggcca
     3361 tcgtgttacc ggtcagttac tttatctttg tcgatcctgt gtgggcaccg cagaacgttc
     3421 ccgttctctg tttgaccata cttgcatctg tcgttatact cattcgccac agagagaata
     3481 tttccaggct catcaaggga acagaacaca agactcgtca tgtttgagca tctacaggag
     3541 atcagtgaac gagccagcat cgagtctatg actcgaactg cagaacaatt cctggacaat
     3601 catcctatag cgcgggcgcg agacctgtat cgctggctct gggaaggcga gtttggcccg
     3661 ggcactcgtc atcagccaac gcgactggat gaacttacac aaatgattcg gaccgctcgc
     3721 atgcataagt cagcgggttc gcagcccatg tgggagcctc tgggcctgac gtgtcgtttc
     3781 gtgcacgtga acttgattcc gtacagcgac gcaggttgcc cgttaaaaag aattctcact
     3841 ctgggagaga gaacacgcga ctatcgtgca gaccagctca gattcaaaat gcagtgggcg
     3901 tttatgaaaa cgcagatcat ccatggaatg cgcataacgg tagaagaaat ggtgtctttt
     3961 gaaaacgaca ttgcatttca catgactccg gagatttcct tcacagatga atacctggca
     4021 gaatatggaa gcggatatat ggtcgttcct tacgatttgt tttttgaaac ctatccagaa
     4081 tatgcggagc acgaatcttg atgcgcattc ctacgggcgt aacgtttgaa gatggaaaac
     4141 tctactatgt agatcagaca aaactgccgg gaaccttcga gaagaaagaa tgcactactc
     4201 tcgaagaagc agaagaagca atccgtcagc tgcgcgttcg aggagcaccg gcgatcgcga
     4261 cgttcgcttc ctttgccgca gccctggaaa tggatcgcaa gagacatctg gcccatccgc
     4321 aatggaaagt agaagcagaa caggtcatag atcggcttct ggccacaaga ccaaccgcag
     4381 tcaatctatc tcacgccatg aaaagattgc gcgagcgact cgccgcagga gacaggagtc
     4441 ttctttcaga agcggctgcc atccaggagg aggacaaaca actctgcgat gccctgggta
     4501 aaagtggaag cacactcatt ccagctggag cgcgcgtcat cacccattgc aatactggag
     4561 ctctggcgac cgccggcatt ggaactgcaa tcgggtcctt ctacacagcg gctgcggagg
     4621 gaaaagatat cacagtctac gcctgcgaaa cccgtccgct tcttcaagga gctcgtctca
     4681 catcctggga actcgatcgg gcggggattc gaacgatcct gatcacagat agcatggctg
     4741 ccttcatcat
//
LOCUS       DAOQJL010000285         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10654_length_4748_cov_3.784147, whole genome shotgun sequence.
ACCESSION   DAOQJL010000285 DAOQJL010000000
VERSION     DAOQJL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_4748_cov_3.784147"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLG78_12290"
     CDS             <1..647
                     /locus_tag="PLG78_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE23943.1"
                     /translation="YTERVRCCIPIASCMAHSAMQIAFNEVGRQAITSDPNWRGGAYY
                     GKARPEHGLAVARMVGHVTYLSEFSMHQKFGRKLQKAASNEDMFPQYSVESYLQHQGE
                     SFVRRFDPNAYLYITKALDNFDLLEGRAPEELLRDLKARFLVISFESDWLYPPSQSRE
                     MVRALNRSNAVVTYTNLETPYGHDSFLIQNPDFTRVLQNFLNTEYDSVSRQALV"
     gene            661..1257
                     /gene="metW"
                     /locus_tag="PLG78_12295"
     CDS             661..1257
                     /gene="metW"
                     /locus_tag="PLG78_12295"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNE23944.1"
                     /translation="MNEYLYDQVIQWIPEGSRVLDLGTGDGSFLARLVKAKKVQGVGV
                     EKEPELVAQCIEKGLVVHQGDIMDGLDQYGVQSFDCVLLLGTFQEIGTPLELLREAYR
                     VGKTVIIAYTNFAYFHIRLQIMFSGRSPITRALPHAWYTSPNIHYFSVEDFDDFCERY
                     KLRVLRNAYFNAAGPVSIFPNLLAEQAVSLLESTPISE"
     gene            complement(1268..2455)
                     /locus_tag="PLG78_12300"
     CDS             complement(1268..2455)
                     /locus_tag="PLG78_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE23945.1"
                     /translation="MMLRLLLALALFPGLAAAGPVRFEFKLEKDDVLAVDKYQDIRIQ
                     KNGEQKSREEKNRIVLKVSDSTPSGAVMEGLFHTYTRSPRLVGEYRRDRDFNSRFIFQ
                     KNGENVVSDDYVMPNLRGLPTFPDKELSAGDKWQAPGLETMDFGSTKIKIPLNVAYQF
                     TGVTHLPEDSKLSQDAPEIQYVYTFHKAISDPRVPFKMIVGHSADRLWFDTNAGVPIF
                     DTNRLTYTFYMRDGQILRMAYRIDSWWKKTRRARPEDRQEIVTDVNRQIAENPNVTVH
                     QTDEGVVLNLNAILFDVDSANLSEQARGDLDRVAEILRKYPDREIRVSGHTDSTGGAA
                     HNVKLSEDRARSVVQNLGKRTGIDSKRFSYRGYGSTKPAADNSTADGRAKNRRVEILI
                     VTD"
     gene            2557..2922
                     /locus_tag="PLG78_12305"
     CDS             2557..2922
                     /locus_tag="PLG78_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE23946.1"
                     /translation="MILLEGLHHISIGSSDLKKSLDFYRDLLDFELLEESSRYILIRL
                     DPITVRLNHIEGYKSPVQNPGEASLSFILDVDDFTDALKELEVKKVEILKGPVMIEGG
                     EAVLIADPDGNLIELFYNE"
     gene            complement(2999..3214)
                     /locus_tag="PLG78_12310"
     CDS             complement(2999..3214)
                     /locus_tag="PLG78_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23947.1"
                     /translation="MDVRSKPEIGMKNPEMFRDLKARKLENPAALPTTPDSERQMVTD
                     EKIAKSVLVSQSKDVFKVTGTQIDKIA"
     gene            complement(3284..>4748)
                     /locus_tag="PLG78_12315"
     CDS             complement(3284..>4748)
                     /locus_tag="PLG78_12315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE23948.1"
                     /translation="HFTKYAFAKENGTGELEQDMARLFAGEAAHRLRSELASRKDEYG
                     ASDALLNDLFSPDMNKKIQELTSRDRYNKVGDAALTGSFGALATEDLKDIRDTYKKIA
                     EQVVMPHAEHVHRNDDFVPDDILQSLLDNGAFGLSIPEEYGGYQDTFGNTGMMIVTEE
                     LSRAGLSIAGSLITRPEILSKAILKGGTPEQKTKWLGALATGEKMCAVAATEPDYGSD
                     VAGFKVTADPVEGGWVLNGVKTWCTFAGYADSLMVLARTEKDPGLKHKGLSILIAEKP
                     RDKGHKFDFAQPSGGKCTGSAIPTIGYRGMHSFEVNFDNYFVPAENLIGGEAGRGKGF
                     YLQMEGFAGGRVQTAARANGVMQASLEAAVRYANDRKVFSKPIADYQLTKWKIARMAM
                     IVQASRQYSYEVARLMDNHQGLMEASLVKFYASRIAEWVAREAMQIHGGMGYAEEYAV
                     SRYFVDARVFSIFEGAEEVMALRVCARDLLAQALGQS"
BASE COUNT         1054 a         1203 c         1191 g         1300 t
ORIGIN      
        1 aatacacaga gcgcgtgcga tgctgcattc cgatcgcaag ctgtatggcg cactctgcga
       61 tgcagattgc attcaatgaa gttggacgtc aggcgatcac ctccgatcct aattggagag
      121 gcggtgctta ctatggtaag gcacgcccgg agcatggtct ggccgtcgcg cgcatggtag
      181 gacacgtaac atacttgtcc gagttttcca tgcaccagaa gtttggacga aagctgcaga
      241 aagccgcctc gaatgaagat atgttcccgc agtactctgt ggagagctac ctccagcatc
      301 agggagagag ttttgtgcga cggtttgatc cgaatgcgta tctctacatc acaaaagccc
      361 tggacaattt tgatctgctt gaaggcagag caccggaaga gcttctacgc gatttgaagg
      421 caaggtttct agtgatttcg tttgagtcag actggctcta tccaccgtct caatcgagag
      481 agatggtgcg agcactcaac agatccaatg cagtggtaac ttatacgaat ctggaaacgc
      541 cgtacggaca cgattcgttt ctcattcaga atccggactt tacgcgagtc ctgcaaaatt
      601 ttctaaacac agagtacgat tcggtttccc gccaggctct cgtctaaaaa ctctgtccca
      661 atgaacgagt acctgtacga tcaggtaatc cagtggattc cagaaggttc gcgggtgctg
      721 gatcttggca ccggcgacgg tagctttctc gcgcgccttg tgaaagcaaa gaaagtgcag
      781 ggagtgggcg tagagaaaga gcctgagctc gttgcgcagt gcattgagaa aggactggtg
      841 gtccaccagg gcgatatcat ggacgggctc gatcagtatg gcgtgcaatc tttcgattgc
      901 gttttgttgt tgggcacatt tcaggaaatc ggcactccac tggagctgtt gcgcgaagca
      961 tatcgcgtcg gaaagacagt catcatcgca tacaccaatt tcgcatactt tcatatacgg
     1021 cttcagatta tgttcagcgg acgttctccc attacgcgtg ctttgcccca tgcgtggtat
     1081 acaagcccca acattcacta tttctctgtg gaagattttg acgacttttg cgagcggtac
     1141 aaactccgcg tgctgaggaa tgcttatttc aatgcggcgg gcccggtctc tatattcccg
     1201 aatctactgg ctgagcaggc cgtatccctt ctggagtcga ctcccatttc tgagtgatgg
     1261 ttcgcgctca gtctgtaacg atcaggattt ccactcttct gttctttgct cgcccatctg
     1321 cagtggagtt gtctgcggcg ggtttcgtgg atccgtatcc tctgtaagag aatctcttac
     1381 tatcaatacc ggttctcttt cccagatttt gcacaacgga tctagcacga tcttcagaaa
     1441 gtttcacgtt gtgcgcggct ccgcctgtgg aatccgtgtg gcctgagact ctgatttcgc
     1501 gatccggata tttgcgcagg atttccgcta cccggtcgag atctccgcgt gcctgttcag
     1561 atagattcgc agagtctaca tcaaatagaa ttgcattgag gtttagaacc acgccttcat
     1621 cggtttgatg aaccgtaacg tttgggtttt ccgcgatctg cctgttgacg tctgttacaa
     1681 tctcctgtct atcttctggt cttgctcttc tggttttctt ccaccaagaa tcaattctgt
     1741 aagccatgcg aaggatctgg ccgtcgcgca tgtaaaacgt atacgtgagt ctgtttgtgt
     1801 caaatatggg tacgcctgcg ttagtatcaa accacagtct gtccgcagaa tgaccaacaa
     1861 tcatcttaaa aggaacgcgc ggatcgctga tggctttgtg aaacgtatag acatactgga
     1921 tttcaggcgc atcctgcgag agttttgaat cctctggcaa gtgagtcacc cctgtgaatt
     1981 ggtaggctac gttcaatgga atcttgattt tcgtgctacc gaagtccatc gtctcgagtc
     2041 caggtgcttg ccatttgtca ccggcggaga gttctttgtc aggaaacgtg ggcagcccgc
     2101 gaagattggg cattacgtaa tcgtcgctga caacgttttc gccattcttt tgaaaaatga
     2161 atctggaatt gaaatcccgg tcccttctgt attcgcccac aaggcgaggc gacctggtat
     2221 aggtgtgaaa gaggccctcc atcacagcgc ccgaaggagt gctatcagag actttcagca
     2281 cgattcgatt cttttcttct ctgcttttct gttcgccatt cttttgaatg cgaatgtcct
     2341 ggtatttgtc cacagccaga acgtcgtctt tttctaattt gaattcgaaa cggaccgggc
     2401 cggcagccgc aagacccgga aaaagagcaa gcgcaagcag cagtcgaagc atcattgaat
     2461 cattcgacgg tcggttgaaa atcttgatag caactttccg gaaaatcgtt gatcgccctg
     2521 agcctggttc aattctgact ggatgcgggg tatagcatga ttctcctcga aggacttcac
     2581 catatttcta ttggatcttc ggatctaaag aaatccctcg atttttaccg ggacttgctt
     2641 gatttcgagc tcctcgaaga atccagccgt tatatcttga tccggcttga cccgatcact
     2701 gtgcgcctca accacattga gggatataaa tccccggttc agaatcctgg tgaggcatcc
     2761 ctttctttca tcctggacgt agacgacttt acagatgcgc tcaaagaact cgaagtaaaa
     2821 aaggtcgaga ttctcaaggg tcctgtgatg attgagggcg gggaagcggt tctgatcgca
     2881 gatccagacg gaaatctaat cgaacttttc tacaacgaat gatgagatca tctacggatg
     2941 atgaaatgaa accggctttc agggccggtt tttttgtgcc atgattttga aagaagagtt
     3001 aggcgatctt gtctatttga gtgccggtca ctttgaatac gtctttggac tggcttacga
     3061 gcacggattt tgcgatcttt tcatccgtca ccatctgtct ttctgagtca ggtgtggtag
     3121 gcagtgccgc cgggttctcc agcttacgtg ccttgaggtc ccgaaacatt tccgggtttt
     3181 tcatgccgat ttctggttta gatcgaacgt ccatgggttc ctcctttcgc aaaaatagaa
     3241 ggctggttca aatcgatcca gccttttttg ccaattcctt acctcagctc tgccccagag
     3301 cctgggccag tagatctctc gcgcagactc gcagtgccat cacttcctct gcgccttcaa
     3361 agatggagaa cacgcgagcg tctacgaagt agcgcgatac tgcgtattct tccgcgtagc
     3421 ccatcccgcc atggatttgc atggcttcgc gtgccaccca ttccgcgatg cgagatgcat
     3481 agaacttcac gagagatgct tccatgagtc cctggtggtt gtccatgagg cgagccactt
     3541 cgtaggaata ctggcgagaa gcctgaacga tcatggccat gcgcgcgatc ttccacttag
     3601 tgagctggta gtctgcgatt ggctttgaaa atactttgcg atcattcgcg tagcgaacag
     3661 ctgcttccag cgatgcctgc ataactccgt tcgcgcgcgc tgcagtttgc acgcggccac
     3721 ctgcgaaacc ttccatttga agatagaatc ctttgcctcg tcctgcttct ccaccgatca
     3781 ggttttctgc aggaacgaag taattgtcaa agttcacttc gaagctgtgc atcccgcgat
     3841 aaccaatcgt tgggatagca gatcctgtac actttccgcc tgagggctgt gcgaaatcga
     3901 acttatgacc tttgtcgcga ggcttttctg caataagaat ggaaagacct ttgtgtttga
     3961 ggcccggatc cttttcagtg cgagcaagaa ccatgagaga gtctgcgtat cctgcaaacg
     4021 tgcaccatgt tttcacaccg ttgagcaccc agccgccttc caccggatct gcagttactt
     4081 tgaatccagc tacgtcagat ccgtagtccg gttcggtagc tgcaaccgcg cacatctttt
     4141 ctccagtggc gagagctccc agccactttg ttttctgctc gggtgttccg cctttcagaa
     4201 tcgccttgct taagatttca gggcgagtga tcaagctgcc tgcgatggaa agtccggcgc
     4261 gcgaaagctc ttctgtaaca atcatcatgc ctgtattgcc aaatgtgtcc tgatatccgc
     4321 cgtattcctc tggaatagac agaccaaacg ctccattgtc caggaggctt tgcagaatat
     4381 cgtccggcac gaaatcgtca ttgcgatgaa catgttctgc atgaggcatc acgacctgtt
     4441 ctgcgatctt cttgtatgtg tcgcgaatgt ctttgagatc ttccgtggcc agtgctccga
     4501 aggatcccgt gagagctgcg tctcccactt tgttgtatcg atcgcgtgaa gtgagttcct
     4561 gaatcttctt gttcatgtcc gggctaaaca gatcgttgag aagtgcatcg cttgcgccgt
     4621 attcgtcttt gcggctggca agttctgatc tgaggcgatg agccgcttct cctgcgaaga
     4681 ggcgtgccat atcctgctcc agttcgcccg ttccattctc tttcgcgaag gcgtatttcg
     4741 tgaaatga
//
LOCUS       DAOQJL010000286         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10728_length_4724_cov_4.612765, whole genome shotgun sequence.
ACCESSION   DAOQJL010000286 DAOQJL010000000
VERSION     DAOQJL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10728_length_4724_cov_4.612765"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..961
                     /locus_tag="PLG78_12320"
     CDS             197..961
                     /locus_tag="PLG78_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNE23949.1"
                     /translation="MFFFLSKVLPGLLFPYPLFLILSGIVLRKMQPGRLRRLFGLALL
                     LVTVASTYVTAAFLMRGLENRYAPLTVEETPTAEAIVVLSGMVTPLTRAKGRPEFSAA
                     VDRILAGRELLAARKAPVLIISGGSGLISQIGTPEAETLRNWMIKDGFAPDKVIAEKA
                     SRNTAENAVETAKIARARGIKKIILVTSAFHMQRSVLCFEKEGFEITPYPVDYYILDE
                     FPGIEAFFPAPAGMTLSGIALKEYAGIIAYRLKGYL"
     gene            complement(990..1658)
                     /locus_tag="PLG78_12325"
     CDS             complement(990..1658)
                     /locus_tag="PLG78_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNE23950.1"
                     /translation="MRQITVYAHRGSALQARENTAAAFDSALHLGFKAFELDLLRLKD
                     GTIVVFHDFTLRRQFGKLKRIRSVTLKEFRKINSDLLTFDEFVDRYAKKKITVNFEVK
                     DDSKTLRAMLPGIAKFHDPVISSFRRTVVDAALCEGLEAGYLFHTMRAFRAGQKKMKG
                     NRLHISKNLLRSRNKPTKVFSSYNTHVYTVNDPIEALSLSAFPFVHGIFTDKPEVMHA
                     FQRK"
     gene            complement(1658..2203)
                     /locus_tag="PLG78_12330"
     CDS             complement(1658..2203)
                     /locus_tag="PLG78_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNE23951.1"
                     /translation="MAGEDVMDEEEGGSGEAPAEAAGSPASGLGKVVKILIYVALGAV
                     GLVIMAVIAYYVASFAAARQYKEVASIAVVKPPPPTENFNFTDDFRVNTADTGDPHFV
                     KLRLSLGYEQGNPALTAELGQRSPQLRNIINLILAGKTREDLRTVEDQLELREEIKAS
                     VNHVLAEGKVSEVYFNEFIVN"
     gene            complement(2300..2521)
                     /locus_tag="PLG78_12335"
     CDS             complement(2300..2521)
                     /locus_tag="PLG78_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNE23952.1"
                     /translation="MIQLHRLNKKAFFLNHRQIEIMEANPHTVITLANENKYIVEESI
                     SDIIALIRDYERQIVADPGSGIHSRTLSP"
     gene            2520..3179
                     /locus_tag="PLG78_12340"
     CDS             2520..3179
                     /locus_tag="PLG78_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE23953.1"
                     /translation="MHVVSRMKSDLSSAFLFCGIYLGVLFITSSLIPLNAWTAALPGA
                     LSTTVCLILAALASRIMPAQMGSFRPLSAAFIGLFLFAAQSRMTEPLPGFLREPVDLP
                     GAFFSMVLLGPLCEEAFFRGLLLDRFGGNARAVVLQAVFFAVSHFSLAGLPFAFTGGL
                     LLGFIAISTRGILSAFVAHATLNAIVISQRILWPETIPLWIIALCFACGLFGIWRFRK
                     A"
     gene            3176..4669
                     /locus_tag="PLG78_12345"
     CDS             3176..4669
                     /locus_tag="PLG78_12345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE23954.1"
                     /translation="MIFSSALFFFFFAAVFTMHWAILPGLVPPRHLRTARHAALLIAS
                     YVFFASYEWIYAFLLLFFTLTAYGSAFYLQPGGSRDKLRGWLLLAVIVTFVGLLGYYK
                     YYNFFLGNLLTLVGIVGYHPDVVFHTIALPLGISFFTFQALSYVIDVWRGKTPVEQSF
                     LRFALFKSFFPQLVAGPIVTAREFLPQLYADKELDLERLRSGARWFALGFIKKAVIAD
                     NVSPAVDMLFANPAGQFLHNSSGAWLAALGFSAQLYADFSGYSDMAWGSAIWLGFDLP
                     ENFRMPYMARSITDHWRRWHMSLARWIREYVYISMGGNRVSFVRHKVNLLFSFVISGL
                     WHGANWTYVVWGTAQGIILVIENTLGEWKKKREEGQPVTEPGAFTRIISVPVQLTLWL
                     YMFIFTTSSHVVFRSPSLSAAWTHLKTMYSFSSQGLAPQPSIYRPILISTVVVLLGQL
                     FGYWYFEKKKFQVKVPIWLEFAFYPFMILALIQLAAPEGGAFIYFVF"
BASE COUNT         1080 a         1175 c         1175 g         1294 t
ORIGIN      
        1 gttatactcg aagcggcgcg taagtcccgg gtttgacgtc gatacttgac gcaagaaaaa
       61 aaaggtcgcg cccgagaaaa ctgcgacgca gtcaacagaa ttgcttccga agcgactgca
      121 gtatgtagtt ctaaagtatt attactgtat tgccatcaat gcatcgcaca aaatggtgcg
      181 tcaggaggtt gtctgaatgt ttttctttct ttcaaaagtt cttccaggcc ttttgtttcc
      241 ttacccgctt ttcttgattc tttctggaat cgttctgagg aagatgcaac cgggtcgttt
      301 gcggcgattg ttcggactgg cactgctgct tgtcaccgta gcatctactt atgtcactgc
      361 cgcgtttctc atgcgtggcc tcgaaaaccg ttatgcgccg ttgacagtgg aggaaacacc
      421 caccgcggag gccatcgtag ttctttccgg aatggttacg ccgctcacgc gcgcgaaagg
      481 aagacctgag ttttccgccg ccgtagacag gattctggca ggaagagagc tccttgccgc
      541 gcgaaaagcg cctgttctta tcatcagcgg cggctcgggg ctgatttcgc agatcggaac
      601 accggaggca gaaacgttgc gaaactggat gatcaaagac ggattcgcac cggacaaagt
      661 gattgcagaa aaggcgtcca ggaacacggc agaaaacgca gttgaaacag ccaaaatcgc
      721 aagagctcgt ggaattaaaa aaatcatcct ggtgacttcc gcttttcaca tgcagcgcag
      781 tgtgctgtgt tttgaaaaag agggcttcga gatcactccc tatccagtgg attactacat
      841 cctggatgaa ttcccaggga ttgaagcgtt ctttccggcc ccggcaggaa tgacgttaag
      901 cggtattgct ctcaaagaat acgcagggat catcgcctat cgcctgaaag ggtatctgta
      961 ggatgcgcgc ctggcacaaa acagaaggac tatttccgct gaaaggcgtg catgacttct
     1021 ggtttgtctg taaagattcc gtgaacaaac ggaaatgctg agagagacaa agcctcgatt
     1081 ggatcattga ccgtgtacac gtgagtattg taagaggaaa aaacctttgt tggcttgttt
     1141 cgagagcgaa ggagattctt tgaaatatga aggcgattgc ccttcatctt cttctgaccg
     1201 gctctgaaag cacgcatagt gtgaaataga tacccggcct caagcccttc acagagggca
     1261 gcatccacga cagtgcgccg gaaagaggaa atgacaggat cgtgaaattt cgcaattcct
     1321 ggaagcatgg cgcgaagagt tttagaatcg tcttttactt caaaatttac tgtgatcttc
     1381 tttttcgcat aacgatccac gaactcatcg aacgtaagca aatcagaatt gatctttctg
     1441 aattctttga gagtgacaga acgaattcgt ttgagtttac caaactgtct gcgcagagtg
     1501 aagtcgtgaa agactacgat cgtgccgtct ttaagacgca acagatcgag ttcgaacgct
     1561 ttgaaaccta aatgaagagc ggagtcaaat gccgcggcag tgttttcacg ggcctgtagt
     1621 gcgctgccgc ggtgagcgta gactgtaatc tgacgcatca gttgacaatg aattcgttga
     1681 aatacacttc ggagacttta ccttccgcga gaacgtgatt gacggaggct ttgatctctt
     1741 cgcgcaattc aagctgatct tctactgtgc gaagatcttc gcgcgtcttg cccgcaagaa
     1801 tcaagttgat aatatttctt aactgtggac ttctttgacc aagttctgct gtaagcgctg
     1861 gatttccttg ttcatatccc agactcagtc tgagttttac gaagtgagga tctccggtat
     1921 ctgcagtgtt gacgcgaaag tcatctgtga aattgaaatt ttcagtcggt ggaggtggct
     1981 taaccactgc aatggatgca acctctttgt actgacgggc tgcggcaaaa gaggcaacat
     2041 agtacgcgat gactgccata atgaccagac ctactgcgcc cagggcaaca tagatcagga
     2101 tttttacaac tttacctaga ccgcttgcag gcgagccagc ggcttcagcg ggagcttcgc
     2161 cactcccccc ttcctcttcg tccatgacat cttcaccggc catgcaatcc tccgactgct
     2221 ccgcccacgg gcagtcctga tttataatcg gtcaatttgg cagaggcatc gcttcttttt
     2281 tacagcgaaa gcagcccgtt cagggcgaaa gagtacgcga gtgaattccg gaaccagggt
     2341 ctgcgacgat ctggcgttcg taatcgcgaa tcagggcaat gatatcagaa atggattctt
     2401 ctacaatata cttgttctca ttggccagag tgatgaccgt atgcggattc gcctccatga
     2461 tttcaatctg gcgatggttc agaaaaaaag ctttcttgtt cagcctgtga agctggatca
     2521 tgcatgttgt ttcccgcatg aaatcagatt tgtcgagcgc attccttttc tgcggcattt
     2581 acctgggtgt tctgttcatt acctcgagcc tcatcccact caatgcctgg acggccgcgc
     2641 tcccaggagc tctttccacc acagtctgcc tgatcctcgc cgcacttgct tcacgcatca
     2701 tgccggcaca aatgggcagt tttcgtcctc tcagtgcggc attcatcggc ctttttctgt
     2761 ttgcggccca gagcagaatg acagaaccgc tgccagggtt cttgcgcgaa ccagtagacc
     2821 tcccgggagc tttcttcagt atggtcctgt taggccccct atgcgaagag gcttttttca
     2881 gaggtttgct actggatcgg ttcgggggaa atgccagagc ggtggtgcta caggcagtgt
     2941 tcttcgccgt gagtcatttc agcctggcag gactcccttt cgcgttcacc ggcggtctgc
     3001 ttctgggttt cattgcgatt tccacgcgcg ggatcctatc cgctttcgtg gctcacgcta
     3061 ctctcaacgc aattgtaatc tcgcagcgca tactgtggcc agaaaccata cctctctgga
     3121 tcatagcgct ctgctttgcg tgcggcctgt ttggcatatg gcgatttaga aaagcatgat
     3181 cttctcaagc gcacttttct ttttcttttt tgccgcagtt ttcaccatgc actgggcgat
     3241 cctgcctgga ctcgttccac cacgccatct tcgcacagcc cgtcatgcgg cattgctgat
     3301 cgcgtcgtat gtgttttttg catcctatga atggatttac gcattcttac tcttgttctt
     3361 cactctgact gcctacggtt ctgcttttta tctccagccg ggtggttcgc gcgacaaact
     3421 gagaggatgg cttcttctgg cagtgattgt cacgttcgtt ggactgctcg gatactataa
     3481 atactataac tttttcctgg gcaatctact gactcttgtt gggattgtgg gctatcaccc
     3541 ggatgtggtt ttccatacaa ttgctcttcc cctgggcatt tcatttttca cattccaggc
     3601 actgagttat gtgatcgatg tttggcgcgg taaaactccg gtggagcagt catttctcag
     3661 attcgcgctg tttaagtcat tcttccctca gctggtggcc ggaccaatcg tcactgctcg
     3721 cgaatttcta ccgcaactct atgcggataa ggaattggat ctagaaagac ttcgctccgg
     3781 cgcgcgatgg tttgcacttg ggtttattaa gaaggcagtg attgcagata acgtttcgcc
     3841 tgccgtggac atgctcttcg caaatcctgc gggacagttc ctccataatt cgagcggtgc
     3901 ctggcttgct gcacttggat tttctgccca gctctacgca gacttctcag gatacagcga
     3961 catggcctgg ggatcggcca tatggcttgg atttgatctg cccgaaaact ttcgcatgcc
     4021 atacatggcc agatccatca cggaccactg gagacgatgg cacatgtctc ttgccagatg
     4081 gatccgcgaa tacgtctaca tttcgatggg tggaaaccgc gtttcgtttg tacgtcacaa
     4141 ggtgaatctt cttttctctt tcgtgatcag cggcctctgg catggagcga actggacgta
     4201 tgtagtgtgg ggaacagccc agggaatcat tctggtcatt gaaaacacac tgggcgaatg
     4261 gaagaagaaa cgcgaagaag ggcagccagt cacagaaccc ggcgccttta caagaatcat
     4321 atctgtgcct gtgcaactaa cgttgtggct ctacatgttt atttttacaa ccagcagcca
     4381 cgtagtgttt agaagcccga gcctttctgc tgcatggact catctcaaga caatgtattc
     4441 tttttccagt caggggctgg cgccacagcc atcgatctat cgtccgattc tgatttcaac
     4501 cgtcgtggtt ctgcttggac agctctttgg ctactggtac ttcgagaaaa agaaattcca
     4561 ggtcaaagtt cccatctggc ttgaattcgc gttctatccg tttatgatcc ttgctctcat
     4621 acagcttgca gctccggaag gcggggcgtt tatatacttt gtgttttaaa attttttcgc
     4681 actttcgtca aagaaaaatt catacagctc ggccaagcgt agat
//
LOCUS       DAOQJL010000287         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10732_length_4721_cov_4.157523, whole genome shotgun sequence.
ACCESSION   DAOQJL010000287 DAOQJL010000000
VERSION     DAOQJL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_4721_cov_4.157523"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3003)
                     /locus_tag="PLG78_12350"
     CDS             complement(<1..3003)
                     /locus_tag="PLG78_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE23955.1"
                     /translation="MSVVRIAGIDFPVSERETEWLPSANLVLTPTTIRNLQKILHPLR
                     AGIPLLLVGDAGVGKNALIYYINALRKHPTIRYSFNEDTLPEDLVGAYRIDPGHSFVW
                     SDGPLTHAIRTGSVFVADEMNLAPPEVLKRFLSVFSEGSIQILEGDSSTLRPAPGFHF
                     IATQNPAEGFEGRKNLPREIQKYFATVYVDAYPDSEVIEILCGTHPTVRADLVEAIVR
                     INGKVETALVKREIGARDLERYHFNLRNMNRLCYRIAGSDGSVAAVQREIYDVYVLPF
                     RNPEDQKIVKEIIHSVIPDRAEESSVRISVGGDAISIGHAILKPAVIDRARIEDSFNH
                     FPPVESRLPLLASIARSVEMHENVLLESDADVEPEEYAEFFARMMGAELTVVHLSRGM
                     HTSDVLGGLKPVRDRELEWVDGPITRAVRNGHYLLIRGLEAAGPELVEKLNMLLDDAR
                     ALLLPPEAGEREPLLLKENSVIFAVKVFRNTKSITTISRAFRNRFSSVIVGATQDPVS
                     LEDIVLSALDSDESARDLAARMVQFHLKIRDHAEKREIGSANIQPYMYGLTNLHRWAS
                     LLVGSNPESGEDLKAIVIRSAGISYLNEISDPMEREKMYQALERILSGLPLDELSQNA
                     DALKKKHLIKRTTFQKKIDWDPSQHFREANTGRAQRKLTGQELKKGVRINTPETGGNT
                     KEGPDAWYGQDTQGNRGQGEPGHGGGGWGYRTEELYQEFLKKRRALWEYNIGVSLQDF
                     HEVFGSEIERVVIDFDRLLDPRSDIHRRYQNHGSRVDGRRYLAYISGKGDGRVFDKTT
                     VVLERDRLKGVEIIFAVNKGRRIFNFEYSIATLVSILSSTIILSNHKVPVAACGYSDL
                     KNQKKDIDLMWFKNLDDPYTNQEEEHLFSGLARDWHGDTVPEADVLVSLADSFSAAAT
                     TRILVMLSDFRGSRAKMKLEDTVASKESVDLKQMTEQMEKRDIHFLGVGLGPKSLAEY
                     FFPEHLSVGGENYANLPILLSNKLSEMIH"
     gene            3075..4169
                     /locus_tag="PLG78_12355"
     CDS             3075..4169
                     /locus_tag="PLG78_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE23956.1"
                     /translation="MLKLSERMSRIDTSKIRRAFDLAAKLENPINLSIGQPHYATPEP
                     ITEAIAAALKDGKTAYTPTQGILPLRERIARKFLEQNKFAASPEHILISSGISSLIQL
                     LFLATIDPGTRVLITDPCFLIYRSLLRYFNANVTTVPEEFTQEDLDRLPPGDFSLIIF
                     ATPSNPTGYVMKKSQIEALAKFADARNALLVSDEIYELFDYEGQFTSAASVHKNTITL
                     SGFSKTYSMTGLRLAAAHGPEAIIKAMTTLQQYTVVCAPAPVQWAGIRALDLDMSSYV
                     KAYKKNRDACVSALSGRVKFPEPGGAFYIFPDVMEDEDSFVERAINEKQLLVVPGSIF
                     CAERTRIRISYAASPETLERGLAAFVSLLK"
     gene            4166..>4721
                     /locus_tag="PLG78_12360"
     CDS             4166..>4721
                     /locus_tag="PLG78_12360"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNE23957.1"
                     /translation="MSEELLLPFAYVFAGLLGAILGSFYYATATRVSYFFYSAARKSY
                     PRKRDRFRALIFKPSFCMECGAPISRVYLIPVFGYAFSRARCAECGTRISPKHLVAEI
                     YLAILLPTLLYFGRSWPEALLMTLFAGQLYVSIVTDTRHMILDHENTAFLFLWAAILT
                     AIADGTTARLIGGGGTLVIFGALYW"
BASE COUNT         1156 a         1246 c         1126 g         1193 t
ORIGIN      
        1 atggatcatt tctgaaagct tattggaaag taaaatgggc aggttcgcgt agttctctcc
       61 acccacgctg aggtgttccg gaaagaaata ctcggccagt gactttggac caagaccaac
      121 acccaggaaa tgaatgtcgc gtttctccat ctgctccgtc atctgcttca aatccacaga
      181 ttctttagac gcaaccgtat cttccagttt catcttcgca cgagacccgc ggaagtcaga
      241 aagcatcacc agaatgcgcg tggtagcagc ggctgaaaaa ctgtctgcga gcgatacaag
      301 cacgtctgct tcgggaacgg tatctccgtg ccagtctcgc gccagtccag aaaacagatg
      361 ctcctcttct tgattcgtat aaggatcatc cagattctta aaccacatca gatcaatgtc
      421 tttcttctga ttcttaagat cagaataacc gcaggcagcc actggcacct tgtgattgga
      481 gagaatgatc gtgctgctta aaatagaaac gagtgtggca atcgagtatt cgaaattgaa
      541 aatgcgcctt cctttgttga cggcgaaaat gatttcaacc cccttgagtc gatctctttc
      601 gagcacaacg gtggtcttat cgaagacccg gccgtcccct tttcctgata tgtatgccag
      661 ataacgtcta ccgtccacgc gagatccgtg attctggtag cgacgatgaa tgtcggacct
      721 ggggtcgagc aatcgatcaa aatcaatcac caccctttct atctcagacc caaagacttc
      781 atgaaagtcc tgcagagaaa cgccaatgtt gtattcccag agcgcgcgcc tcttcttcag
      841 gaattcttga tatagttctt ctgtgcgata gccccatccg ccaccaccgt gtccgggttc
      901 gccctgccca cgatttccct gggtgtcctg tccgtaccat gcgtccggac cttcttttgt
      961 gtttccaccg gtctccggcg tattgatgcg aacacccttc ttcagctctt gcccggttag
     1021 cttgcgctgg gcgcggcctg tatttgcttc gcgaaaatgc tgagatggat cccagtcgat
     1081 tttcttctga aaggtggttc gcttaatcag gtgttttttt tttaacgcat cagcattctg
     1141 gctgagctcg tcgagtggaa gaccggaaag aatccgttcc agagcctgat acatcttctc
     1201 tcgttccatt ggatcgctga tttcgttgag ataggaaata cccgcagatc gaatgacgat
     1261 cgcctttaga tcttccccac tttcaggatt gcttccgaca agcagactgg cccatctgtg
     1321 gagattggta aggccataca tataaggttg aatattggca gaaccgatct cgcgcttttc
     1381 tgcatgatca cgaatcttca aatgaaactg aaccatgcgt gcggccagat cacgtgcact
     1441 ctcatccgaa tcgagagcag acagtacaat atcttcaagg ctgaccggat cctgagtcgc
     1501 gccaacgatc actgagctaa agcgatttcg aaatgctctg gaaatagttg taatactttt
     1561 agtatttcta aataccttaa cggcaaaaat tacagagttc tctttcagga ggagaggttc
     1621 cctttcgcct gcttccggtg gaaggagaag agctcttgca tcatccagga gcatgttcaa
     1681 cttttctacg agctccggtc ctgcggcttc cagtccgcga atcagcagat aatggccgtt
     1741 tcgcactgct ctggtgattg gtccgtccac ccattccagt tcacggtcgc gaaccggctt
     1801 aagaccaccg agaacatcag aggtgtgcat tcctctagac aaatgcacaa cggtgagctc
     1861 cgcgcccatc atcctcgcga agaattcagc atattcctct ggctccacat ccgcgtcgct
     1921 ttcgagaagc acgttttcat gcatttccac agaacgcgcg atggaagcaa gaagcggcaa
     1981 tcgactctct accggtggaa aatgattgaa agaatcttca atccgagcgc gatcgatcac
     2041 tgcaggcttg aggatggcat gtccaatgga gattgcatcc ccacccacgg agattcgcac
     2101 ggatgattcc tctgcacgat ctggaattac agaatggatg atttctttta ctattttttg
     2161 atcttcagga ttgcggaaag ggagaacata cacatcataa atttcgcgct gcacggcggc
     2221 cacggatccg tctgaacctg caatgcgata gcacaggcga ttcatattgc gcaggttaaa
     2281 gtgatatctc tcaagatctc gcgcaccaat ctcgcgcttt acaagtgccg tttctacttt
     2341 gccattgatg cgcacaattg cttctaccag atccgcgcga acagttggat gagttccgca
     2401 gaggatttcg attacttccg aatccggata cgcatccaca tagacagtgg cgaaatactt
     2461 ctggatttcg cgcggtaggt tttttctgcc ctcgaatcct tccgcgggat tctgcgttgc
     2521 gatgaaatga aaaccaggag caggcctgag agtagacgaa tctccttcaa gaatctggat
     2581 gctgccttct gaaaaaacgg acaggaatcg cttcaacact tctggcggcg ccagattcat
     2641 ttcatcagcg acaaagacag aaccagtgcg aatcgcatga gtcagcggac catcggacca
     2701 gacaaaagaa tggccggggt caatgcgata cgcacccacg aggtcttctg gcagagtgtc
     2761 ttcattgaaa ctgtaacgaa tggttgggtg ctttcgcaga gcgttgatat aatagatgag
     2821 agcgttcttg cctacacccg cgtcccctac gagcaagaga ggaatgccag cgcgaagagg
     2881 atgcaggatc ttctgcaaat tgcgaatggt ggtgggtgta aggacaaggt tagccgaagg
     2941 aagccactcg gtttcccgct cgctcactgg aaagtcaatt ccggctatgc gcacaacgct
     3001 catactctaa agcggacctg aaaccatccc gcgcccaggc tggttcttgg aaacaatatt
     3061 tttcggagga tgcgatgctg aaactctcag aaaggatgag tagaatcgac acgtcgaaaa
     3121 tcaggcgcgc gtttgacctg gccgcgaagc tcgaaaatcc aatcaatctg tccattggcc
     3181 agccgcacta tgcaactcct gaaccaatca cagaggcaat cgcggccgcg ctgaaagacg
     3241 gaaaaacggc ctatacgccc actcagggaa ttcttccgtt gcgagaacga atcgcgcgaa
     3301 aatttttaga gcaaaacaag ttcgctgcat ctccagagca catcctgatt agctccggca
     3361 tctccagtct aatccagctc ttgtttctgg caacgatcga tccaggcact cgcgtactga
     3421 tcacggatcc ctgctttctt atttatagaa gcttgcttcg ctatttcaat gcgaacgtga
     3481 ccactgtccc ggaggaattt acacaggaag atctggatcg tctgcctccc ggcgacttct
     3541 ctttgatcat ctttgccaca ccttctaatc caactggtta tgtgatgaag aagagccaga
     3601 ttgaggctct cgcaaagttt gcagatgcgc gaaacgcact tcttgtatca gacgaaatct
     3661 atgaactgtt tgattacgaa ggccagttca cgagtgctgc ctccgtccac aagaacacaa
     3721 tcactctaag cggcttttca aaaacataca gcatgacggg tcttcgcctg gccgcggcgc
     3781 acggaccaga agcgatcata aaggcaatga ctacactgca acagtatact gtagtttgcg
     3841 caccggcccc ggtccaatgg gcaggcattc gcgctctgga cctggatatg agttcgtacg
     3901 tgaaggctta caaaaagaat cgggatgcat gtgtgagtgc tctctccggg cgtgtgaagt
     3961 ttcccgaacc aggtggcgcg ttctatatct ttcctgacgt gatggaagat gaagattcat
     4021 tcgttgagag ggctatcaat gaaaagcaac ttctggtagt gcccggcagc atattctgcg
     4081 ctgagcgcac acgaattcgc atttcgtacg cggcatcccc tgagacttta gagagaggcc
     4141 tcgcagcttt cgtgagcctt ttgaaatgag tgaggagctc ctcctccctt tcgcttacgt
     4201 ctttgcaggg ttgctcggtg ccattctggg cagcttctac tacgcaaccg caacgcgcgt
     4261 gtcgtatttc ttctattccg cagcgcgaaa atcctatccg cgcaagagag atcggtttcg
     4321 cgcgctcatt ttcaaaccgt ctttttgcat ggaatgcgga gcacccatct ctcgtgtata
     4381 tttgatccct gtttttggat atgcattctc acgcgcacga tgcgcagagt gcggcaccag
     4441 aatctcaccc aaacatttag ttgcagaaat ttatcttgcc attctgctgc ccactctcct
     4501 ctattttggc aggtcctggc cagaagctct cctcatgact ctgtttgccg gccaacttta
     4561 tgtgagcatc gtcacagata ctcgacacat gatccttgat catgaaaaca cagcctttct
     4621 tttcttgtgg gcagccattc tcacagcaat cgcagatgga acgaccgctc gcctgatcgg
     4681 aggtggtgga actctagtca tcttcggagc gctgtactgg a
//
LOCUS       DAOQJL010000288         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10741_length_4718_cov_4.717778, whole genome shotgun sequence.
ACCESSION   DAOQJL010000288 DAOQJL010000000
VERSION     DAOQJL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10741_length_4718_cov_4.717778"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..517)
                     /gene="fabZ"
                     /locus_tag="PLG78_12365"
     CDS             complement(26..517)
                     /gene="fabZ"
                     /locus_tag="PLG78_12365"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNE23958.1"
                     /translation="MAISKTPVKINRIKEILPHRYPFLLVDRVVELTESKIVAFKNLT
                     ANEEFFEGHFPVAPIMPGVLQVEALAQTAGLHYPLAFPEEAEKNKDKIGVFTKVNNCT
                     FEKPVIPGDQLRLEVTITEIEKDKNGDPRRITARGVASVDGKTTCQTDLEFSMIPAKL
                     IGR"
     gene            731..1231
                     /locus_tag="PLG78_12370"
     CDS             731..1231
                     /locus_tag="PLG78_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23959.1"
                     /translation="MGDVNIEFINWQLPAQVAQAKLIESVFRTAGFFDRVEVQPGGTG
                     WSIEIVNLEYPGEITLGEKFEQRPLQESFGFINRLVAGRTFLLFPIYHPVDRFVRFSV
                     YHRGIHVRDYDYQSRAHIFVGWLTLLATPWSESRELQLEMVTIARTFMAEATRDGLFR
                     REGKSR"
     gene            1228..1767
                     /locus_tag="PLG78_12375"
     CDS             1228..1767
                     /locus_tag="PLG78_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23960.1"
                     /translation="MIRVRKVGFGITLVSLSIFNMNCASFRSDVTLVSPANEYAIDKD
                     FSFSVVGWREGDRTGTFLKEQMGHANDAERAAVRLRASREELDAGSPLRILNAIVTIV
                     SGGIIPFYHPTKTRISYIAFEDGMMRGECSYEIKNNELFSLLMIPFMPFFWPASQQRN
                     MVKETLDKASECFRLKQAH"
     gene            1833..2831
                     /locus_tag="PLG78_12380"
     CDS             1833..2831
                     /locus_tag="PLG78_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23961.1"
                     /translation="MNSVPLSAQNPVAFHLEHEGPLGIFDPSFAQSPDGIFWMSYSSV
                     DFGWSDPRNGAVSTRLARSNDAVRWSVMAGALNPSVSKGASRQTVHEVSNLVFDASAP
                     ENEKWKIFWHEYEWVAGERDFRHGWISMRTSANPEGPWSSRKKLFVGWGFLSDGQDEP
                     LIRLDLLHADLGGCVAFTEPAAIYHDGSLYLALTCAQILKSRGRIILLRESGSEWTYV
                     GTILTNDADAAPTGYDGFSAPEFAQVGPQLHLIVTPTIGDIYAGCAIYSIEDLRTASV
                     RRLSGRATLSRFIAGPARFQGACGFRGGSSPDLYFGEAHFDELPIFHLFRFPFPDL"
     gene            2973..3740
                     /locus_tag="PLG78_12385"
     CDS             2973..3740
                     /locus_tag="PLG78_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNE23962.1"
                     /translation="MRTGECSIALCSWRRTVVGAIVFAIGSCTQGLPGHEKETRGDLA
                     LILVGLTPNLSGLVARYTFTGSANDSVSGRNLSVGAGAPTLTADRFGTPASAYSFNGA
                     SYFTASDSVPTALPSGSNPRTVCIWYNLTTLSGVTLTNYGTGVAGQAFGLGTNSLFSS
                     VFTYTYDLNIFLSPRTNVWTHYCGVYSGSDIKACINGALVAETTATSSGINTVLSSLA
                     VGKSNFQADFLTGSLDDLRIYNRALSAIDVAAIYSGW"
     gene            complement(3798..4427)
                     /locus_tag="PLG78_12390"
     CDS             complement(3798..4427)
                     /locus_tag="PLG78_12390"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE23963.1"
                     /translation="MSAKKIILGVVIALVTIVVGGYFYLRSSLPRKEDFANLVEPRIS
                     TMQEITVLRVDFNGDADTVISDAYTRLFSAYYKLEGVPKGSSPAPLARYENLANVDFA
                     HTEDLKKIPWKGFAAIQIPDQTNLNDAARAAGATIEKLPYGEVAEIVHFGPYSTEHPT
                     ILKLHRFIESRGYKISGLHEEQYYKGPGMFIPVKPEDYITVVRYQISRK"
     gene            complement(4430..4588)
                     /locus_tag="PLG78_12395"
     CDS             complement(4430..4588)
                     /locus_tag="PLG78_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23964.1"
                     /translation="MQIGETLLSKGIINRDQLEKALDEQKKTGARLGDVVAQLGFATK
                     DQIEAALK"
BASE COUNT         1019 a         1138 c         1241 g         1320 t
ORIGIN      
        1 gggtagtcag aatttgcaag atcgtctaac gtccaatgag ttttgccgga atcatcgaaa
       61 actcgaggtc agtctggcat gttgttttgc cgtccacact tgcaacacca cgtgcagtga
      121 ttcgtcttgg atcgccgttc ttgtcttttt caatttccgt aatggttact tccaggcgga
      181 gttgatcacc agggatcacc ggcttttcaa acgtgcagtt gttcactttt gtgaaaaccc
      241 caatcttatc tttattcttt tctgcttctt cgggaaaagc gagagggtag tgcaatccgg
      301 cagtttgtgc gagcgcttct acctggagaa ctcctggcat gatgggtgcc accgggaagt
      361 gaccttcaaa aaactcttcg ttggcggtta gattcttgaa cgctacaatt ttagattcgg
      421 tgagttctac aacacgatcc actaaaagga acggatagcg atgaggaaga atttctttga
      481 ttcgatttat ttttacgggt gtttttgaga ttgccatgtt tcgatccggt gacggtaggt
      541 tggattgtca atcagcatcc gtattcggcg accggaacaa tcgctggact atcgggggca
      601 attcgggaaa ccatgacgca tgaaatgctc ggcctgtaga ctattggcgg cggcatgttt
      661 tgctttcgtt tcggattgtg ctgtgtttat ctctcaaagg ccggcgcctc catctccgcc
      721 cgaacagcca gtgggagatg tcaatatcga gttcatcaac tggcagttgc ctgcgcaggt
      781 cgcgcaggcg aagttgatcg aaagcgtatt tagaaccgct ggcttttttg acagagtgga
      841 ggttcagccc gggggcacag gttggtccat tgaaattgta aacctggagt atcccgggga
      901 aatcaccctt ggagaaaaat ttgaacaaag gcccctccag gagagcttcg gcttcataaa
      961 tcgcctcgtg gcgggtcgca cattccttct ttttcccatt tatcatccgg tggatcgctt
     1021 tgtgcgattt tcagtctatc acagagggat ccatgtgcgt gactacgatt accagagtag
     1081 agcgcatatc ttcgtaggct ggctgacttt gctcgccaca ccgtggtctg aatccagaga
     1141 gttgcagctg gagatggtta cgattgcgcg cacgtttatg gcagaggcca cgcgcgatgg
     1201 tttgtttcgc cgcgaaggaa agagcagatg attcgcgtac gcaaggttgg tttcgggatc
     1261 acgcttgtct ctctttctat cttcaacatg aattgtgctt cgtttcgctc tgacgttact
     1321 cttgtatcgc ctgccaatga gtatgcgatc gacaaggatt tttccttttc tgtggttggg
     1381 tggagagaag gagatcgtac cggcacgttt ttgaaagagc agatgggtca tgcgaacgat
     1441 gccgagcgtg cggctgttcg tctacgcgca tccagggagg agttggatgc gggaagtccg
     1501 ctgagaattc ttaatgccat cgttacgatt gtttctggcg gtatcattcc gttctatcat
     1561 ccgaccaaaa ccaggatttc gtacatcgca tttgaggacg gaatgatgag aggcgagtgt
     1621 tcctacgaaa ttaaaaataa tgaactattc agtttactta tgattccttt tatgccgttc
     1681 ttctggccag cctcgcaaca gagaaacatg gtcaaagaga ctctggataa ggcgtcagaa
     1741 tgcttccgat tgaagcaagc gcattgaggt ttctcgtgaa ggttctttgc gctacactgt
     1801 ttctctcctt gttgccagga tgcagcgcgc gcatgaacag cgttcctctc tcagcgcaga
     1861 atcctgtggc ttttcatctc gagcacgaag gacctctagg aattttcgat ccgtctttcg
     1921 cacaatcgcc agatggcatc ttctggatgt cttattcgtc cgtggatttt ggatggtcag
     1981 atccgcgaaa cggtgcagtc tctacacgtc ttgcaagatc gaacgacgca gtacgatgga
     2041 gcgtaatggc aggcgcactc aatccttctg tcagcaaggg cgcatccagg caaacggtgc
     2101 acgaagtatc gaacctggtt tttgacgcaa gtgcgccgga aaatgagaaa tggaaaatct
     2161 tctggcatga atacgaatgg gtcgcgggcg aacgcgattt caggcatggt tggatttcta
     2221 tgcgaaccag cgcgaatccg gaaggcccct ggtcttctag aaagaaactc tttgtgggct
     2281 ggggattttt gtcggatggc caggatgagc ccctgattcg ccttgatttg ctgcacgcag
     2341 accttggtgg atgcgttgcc ttcacagaac cagcggctat ctatcacgat ggatctctct
     2401 atctcgcact tacatgcgca cagattctga aatccagagg gagaatcatt ttgcttcgcg
     2461 agtcgggaag tgaatggacg tacgttggga ctatattgac gaacgatgcg gatgctgcgc
     2521 ccacaggcta cgacggcttt tctgctccag aattcgcgca ggttgggccc cagctgcact
     2581 tgattgtaac tcccacgata ggcgacattt atgccggctg tgccatttat tcgatcgagg
     2641 atcttcgcac agcgtccgtc cggagattat ccggccgtgc gaccttgtcc aggttcattg
     2701 ctggccccgc gcgatttcaa ggagcgtgcg gctttcgcgg cggtagttct ccagatctct
     2761 atttcggcga agctcacttt gatgagcttc ctatctttca tctgttcagg ttcccttttc
     2821 cggatctctg aatacttgct cgaaatctat aataacccta aaccattaat tttttttagc
     2881 aacgaggccc cggagattct cgatttgcgt cgcctgtgtt cttcagttgc acctgccaaa
     2941 ggccgatggt cagaatcagg gctcctggca ggatgagaac aggcgaatgc agcatcgcgt
     3001 tatgcagttg gcgtcgcact gtcgtcggtg ccatcgtatt tgcgattgga tcgtgcacgc
     3061 agggcttacc ggggcacgag aaggagactc ggggagacct cgctcttatc ctggtcggtt
     3121 tgactccgaa tttatcaggg cttgtggcgc gttatacctt tacaggaagt gcaaatgact
     3181 ctgtgtctgg tcgcaatctg agcgtcggag cgggagcgcc cactttgacc gcggatcgat
     3241 ttggaacccc tgcgagtgcc tattctttta atggagcaag ctatttcacc gcgtctgatt
     3301 ccgttccaac agctcttccg tccggaagca atccgagaac tgtttgcatc tggtacaatc
     3361 tcacgaccct atctggcgtg actttgacca attacggtac aggggtggcg gggcaggctt
     3421 ttggcctcgg tactaactct ctgttcagct cagttttcac ttacacgtac gatctgaata
     3481 tctttctgtc accgcgaacc aatgtgtgga cgcactattg tggagtgtac tccggctctg
     3541 acatcaaagc atgtatcaat ggcgctctcg tcgcagaaac tactgcgacg tcgtctggaa
     3601 tcaacacggt tctctccagt ctggcggtgg gaaagtcaaa ctttcaggcg gattttctga
     3661 ctggctccct ggacgatctt cgtatttaca atcgagcgtt aagcgccatt gatgtggctg
     3721 ctatctactc tggctggtga tcggtttcaa acgggcccgg tctgaaggaa ggttcgcaaa
     3781 gcctggtttc agatggctca cttgcgcgat atctgataac gaaccactgt gatatagtct
     3841 tctggcttca caggaataaa cattccgggt cctttgtagt attgctcttc atgcagcccg
     3901 gaaatcttgt atcccctgga ttcaataaaa cgatgcaact ttagaatggt tggatgctcc
     3961 gtgctgtaag ggccaaaatg cacaatttcc gctacttcac cgtaaggtaa tttctcgatg
     4021 gttgcgcccg cggcgcgcgc cgcatcgttc aaattggttt gatctggaat ctggattgcg
     4081 gcaaaacctt tccatggaat cttcttgaga tcttccgtgt gtgcgaaatc cacattggcc
     4141 aggttctcat accgtgcgag aggggcaggc gaagatcctt ttggtacgcc ttcgagttta
     4201 tagtacgcag agaagagcct ggtgtatgca tccgaaatga ctgtgtctgc gtctccgtta
     4261 aagtcaacgc ggaggactgt gatttcttgc attgtggaaa tgcgcggttc cactagattt
     4321 gcaaagtctt ccttcctggg aaggctggag cgcagataga agtatccgcc gaccacaatc
     4381 gtaaccagcg cgatgactac tccgaggatg atctttttcg cactcatgac tatttcagtg
     4441 cagcttcgat ctggtctttc gtggcaaaac caagttgcgc aactacatct cccagacgcg
     4501 cacccgtttt cttttgttca tccagagcct tttctagctg gtctcggttg atgatgcctt
     4561 tgctgagaag tgtttcgccg atttgcatgg cgcacgttga atttactgga tgatttgtcc
     4621 atccactttg agccgatcgt cgcgcggaga cttttttggc atgtcttcag gcctgacacg
     4681 cgtcatgttt cactattgat ttctgtcata cggactga
//
LOCUS       DAOQJL010000289         4706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10789_length_4706_cov_3.822404, whole genome shotgun sequence.
ACCESSION   DAOQJL010000289 DAOQJL010000000
VERSION     DAOQJL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_4706_cov_3.822404"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PLG78_12400"
     CDS             <1..1461
                     /locus_tag="PLG78_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE23965.1"
                     /translation="GRINSLITRLQEEVMRVRLQPLRVFFQRLPRLVRDTAAATQKEV
                     RLVILGDSVELDKRLLDTIADPLVHMIRNAIDHGIESSAERLQKGKPAAGTLEVKAQL
                     MGGNVVIQIEDDGRGLNHRAILQAAEAHGLVDSVRAASLSPDEIAEFIFHSGLSTKSK
                     VTETSGRGVGMDVVRTSFESAGGSIVVRSQPGAGTQFIASLPQTLSIITCLIVRAGSQ
                     RFAIPQMNAVELVRFDPSAVAQMSSATVYRLRSRLLPIIDLKQLFTDEPSEKIGFLVV
                     VRSERGLYGLTIEGLDNPEEILVKPLGRQLQEIGLYAGAAVLADSSAVPILDTGGIAR
                     SVNLSSSGIDSVAQSAEDAITDAAFAGLVFQYGGSEFALPVESFPRVEKRTRYQEESS
                     LGKEVVHYTSGPIPLIRLESLLPVPASDGAFLVIFTRGGRTAAIVADEALRVGNDLPP
                     VELGLDPGSPILGRCVVDSRTIVVLSPEVLLNGGAS"
     gene            1458..1877
                     /locus_tag="PLG78_12405"
     CDS             1458..1877
                     /locus_tag="PLG78_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE23966.1"
                     /translation="MSIHRQYLVFCMRGVRYGVALDSCHEVNLRSRVYPVPRARPHIS
                     GIMNLRGEVVTVIDLPSMFSPSKGEASSGAVIRLKAGEHPIAIRVDAVEDILTTENAL
                     EPPPPHLAEEHLRLIAGVIQTENGIVQIIDPQGILRV"
     gene            1889..3556
                     /locus_tag="PLG78_12410"
     CDS             1889..3556
                     /locus_tag="PLG78_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNE23967.1"
                     /translation="MKAQGMKDAEKKQMNVTLTGKERIMDDGDILVSMTDLKSRVVYA
                     NKAFLKIAGYTQEELVGKPHNVVRHPLMPRSAFKDFWQTLQAGKPWNGLVVNRATNGD
                     HYWVDANVAPRFENGNLVGYMSVRRKPQRDQIEKAARLYRSVLEGKATVPSSLRKRMS
                     LKAKFWLFLVFVTVVVMGLGLDAAFDLLHGKVAVGLSVVFSVLTLAVGAGLSNSVLRP
                     IKEAADYARLIATGDLSVRIPHDRNDEVGDLFQALLSMLVNTAAVIGQIKESVESLDS
                     AAGNLTSASTSLSAGLDKVAQQSETFAGIASAATQMNTNLQSVTSSTEEMSISIQEVA
                     RKSSDSATVAEKAQITSREMNSLVKELGVNARDIGKVIESISEIASQTNLLALNAAIE
                     AAGAGDAGKGFAVVASEVKELARQSAESSEEIKDKIGAIQGSTENVIAAIGSITSVIS
                     QINEIAGSIASAVEEQAITTREIASNINQTAQASNDVTASINNVSTAVLSGAQESASF
                     TRLAVDLKDLAGNLNNLVTRFKLSSNGAGTGTIQTMQGSQEMTPEFV"
     gene            3556..4581
                     /gene="cheB"
                     /locus_tag="PLG78_12415"
     CDS             3556..4581
                     /gene="cheB"
                     /locus_tag="PLG78_12415"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNE23968.1"
                     /translation="MQPRISVLIVDDSAAYRNALRDVLASDPGLDVVGQASNGRLALP
                     RIRYHKPAVVILDQEMPEMDGLSTLAAIREEWPAVHVIMFSSRTQRGAETTVKALQLG
                     AADFLAKPAEKDTDIPLYLRSHLLPRIHALGQRFSLSYSSHMRSNVTGDFKVAALGIS
                     TGGPPVLKTILNKLNRGLHASVLIVQHMPPVFTAQLARSLSETSGIPVKEAEDAEPLA
                     PGFAYIAPGGRHLEVSSDGSQLRITDGDPELSCRPSVNVLFRSVARAFGPQALGLILT
                     GMGEDGYSGIQEIRARGGKIIAQSQDSCLIFGMPARPVREGLVDAVLSPDKIAERLNE
                     LFAGVRT"
BASE COUNT         1126 a         1161 c         1300 g         1119 t
ORIGIN      
        1 ggaagaatca attcattgat cacgcgactt caggaagaag tcatgcgagt ccgtctgcaa
       61 ccgcttcgtg ttttcttcca gagacttccg cgtcttgtga gagataccgc ggccgcgacg
      121 caaaaggaag tgagactcgt catcctgggg gattcagtag aactggataa acgtcttctg
      181 gacacgattg ctgatccgct tgtgcatatg attcgcaatg cgatcgatca tgggattgaa
      241 tcgagtgcgg aacgtttaca gaaggggaag ccggcagctg gtactcttga agtcaaagcg
      301 cagctgatgg gcggcaacgt tgtcattcaa attgaagacg acggcagggg tctcaatcac
      361 cgcgcgatcc tgcaggcagc ggaagcgcat ggacttgtgg attctgtccg tgctgcatct
      421 ctttcgccag atgaaattgc tgaatttatt tttcattccg gtctgagtac taaatcaaag
      481 gtaacagaaa catccggtcg aggcgtgggc atggatgtgg tgcgcacgag ttttgaatca
      541 gcaggtgggt cgattgtggt tcgcagtcag cctggtgctg gaactcagtt catcgcctct
      601 ttgccgcaaa ctctttctat catcacttgt ttgatcgttc gcgcaggatc gcagaggttt
      661 gccatcccgc agatgaatgc agtcgagctt gtgcgatttg atccgtccgc agtcgcgcaa
      721 atgagcagtg caaccgtata tcgtcttcgt tctcggctct tgccaatcat tgatctaaaa
      781 caactattta cagacgagcc atctgagaag attggatttc tcgtcgtggt tcgctccgag
      841 cgcggactgt acggtttgac catcgagggc ctggataatc cggaagaaat tctcgtgaaa
      901 ccgctggggc gtcagcttca ggaaatcgga ctctatgccg gtgcggcggt cctcgcagac
      961 agcagtgcag tgccgattct agacacgggt ggaattgcgc gttcggtgaa tctatcgagt
     1021 tccggcattg attccgttgc gcaatccgcg gaagacgcaa ttacggacgc tgcctttgcc
     1081 ggactggtct ttcaatatgg cggatccgaa ttcgctcttc cggttgaatc gtttccgaga
     1141 gtggaaaagc gcactcgtta tcaagaagag agcagcctcg gaaaggaagt ggtccactac
     1201 accagtggtc cgattccact cattcgcctg gaaagtttgc ttcccgttcc agcaagcgat
     1261 ggtgccttcc ttgtgatttt cactcgaggc ggacgcacgg cagcgatcgt tgcggacgaa
     1321 gcgttgcgag ttggaaatga tctgcctcct gttgaactcg gtctcgatcc aggttcccct
     1381 atccttggcc gatgcgttgt cgattcaaga accattgtag tgctttcgcc tgaagtgctt
     1441 ctgaacggag gcgcatcatg agcattcatc gtcaatacct ggtgttttgt atgcgaggag
     1501 tacgctacgg cgtggctctc gattcctgtc atgaagtgaa tcttcgttct cgcgtttatc
     1561 cagtaccgcg tgcgcgccct cacattagcg gcatcatgaa tctgcgcgga gaagtagtta
     1621 cagtcatcga tctgccgtca atgttttccc cttcgaaagg ggaggcatcg agcggcgccg
     1681 tgattcgact gaaggcaggc gagcatccga tcgcgattcg cgtcgatgcg gtggaagata
     1741 tactgacaac agagaacgct ctagaaccgc caccacctca tctggcggag gagcacctgc
     1801 ggctgatcgc cggtgtgatc cagactgaaa atggaatcgt acaaatcatt gatccacaag
     1861 gaattcttag agtttagtcg gaggaagcat gaaggcacaa gggatgaaag acgctgaaaa
     1921 gaagcaaatg aatgtaacgc tcacaggaaa agagcgcatc atggatgacg gagacattct
     1981 agtgtctatg acagatttga agtcgagagt agtgtatgcg aacaaagctt ttttgaagat
     2041 cgcaggctac acgcaggagg agctggtagg aaagccgcat aacgttgtgc gtcatccttt
     2101 gatgccacgt tccgctttta aagatttctg gcaaacactc caggctggaa agccatggaa
     2161 tggcctcgtc gtgaatagag ccacgaacgg ggatcattac tgggtggatg cgaacgtggc
     2221 accgcgattc gagaatggaa atctggtcgg ttatatgtcc gttcgccgca aaccgcaacg
     2281 ggatcagatt gaaaaggccg cgcgcctgta ccgcagcgtt ctggagggaa aggcaaccgt
     2341 cccttcttct ctgcgcaaac gaatgtcttt gaaagcgaag ttctggttat tccttgtatt
     2401 cgtgactgtc gtagtgatgg gccttggcct ggatgcagca ttcgatttgc ttcacggtaa
     2461 ggttgctgtc ggcctttccg tagtcttttc cgtattgact cttgcggtcg gcgccgggct
     2521 gtcaaattct gttctccgcc cgattaagga agctgcggat tatgctagat tgatcgctac
     2581 tggagatttg agcgtgcgaa ttccgcatga tcgcaatgac gaagtagggg atctattcca
     2641 ggctctgctc agcatgctcg tgaacacggc tgccgtgatt ggtcaaatca aggagagcgt
     2701 ggagtcactg gattctgccg ccggaaatct aacttccgcc agtacgagtc tctctgccgg
     2761 attggataaa gtggcgcagc agtctgagac tttcgcaggt atcgcgagtg ccgcgactca
     2821 aatgaatacg aatcttcaat ctgtgacttc ttcgacagaa gagatgtcca tttccattca
     2881 ggaagtggcg aggaagtcct ccgactctgc aactgtcgca gaaaaggcac aaatcacttc
     2941 gcgcgaaatg aacagcctgg tcaaagagct tggagtcaat gcacgcgata tcgggaaggt
     3001 aatcgaaagt atttccgaga tcgcatccca gaccaatctt ctcgcactca atgccgctat
     3061 agaagcggcc ggtgcgggcg acgcaggcaa aggattcgca gtggttgcct cagaagtcaa
     3121 agagctggcc aggcaatcgg ctgaatcttc ggaagaaatc aaagacaaga ttggcgcgat
     3181 tcagggaagc acagagaacg tgatcgccgc aatcggaagc attacaagcg tcatttcgca
     3241 aatcaacgaa attgccggat cgatcgcaag cgcagtggaa gagcaggcca tcactacgcg
     3301 cgagatcgca tccaacatca accagacagc ccaggcatcc aatgacgtaa ccgcgagtat
     3361 caacaatgtt tctacggcag ttctctccgg cgcacaggaa tctgccagct tcactcgtct
     3421 ggctgtggat ctaaaagatc tggcgggaaa cttgaacaat ctagtgactc ggttcaagtt
     3481 gtcgtctaac ggtgcgggaa caggcactat ccaaactatg cagggatcac aagaaatgac
     3541 tccagagttc gtttgatgca acctcgcatt tccgttttga tcgtggacga ctctgcagcg
     3601 tatcgaaacg cgctgcgaga tgttctggcc agcgatcccg gattggatgt ggtaggccag
     3661 gcctccaacg gtcgcctggc tttgccgcgc atccgttatc ataagccagc cgtggtaatt
     3721 ctcgatcagg aaatgccgga gatggacggc ctttccacgt tagccgctat tcgcgaagaa
     3781 tggcctgcgg ttcacgtgat catgttcagt tccaggactc agcggggcgc agagactacg
     3841 gtcaaggcat tgcaactggg agccgccgat ttcctcgcaa agccggcgga aaaagacact
     3901 gacattcctc tgtatcttcg ctcgcacttg cttccgcgca ttcacgcact cggacaacgg
     3961 ttctctctaa gttattcttc acatatgaga tccaatgtga cgggagattt caaagtggca
     4021 gcactcggaa tttccacagg cggcccgccg gttctaaaga cgattctaaa caaactcaac
     4081 cgcggtttgc acgcctccgt tctcattgtt caacatatgc cgcctgtatt tacagcccaa
     4141 ctggctcgtt ctttgagtga gacttcagga attccagtga aggaggcaga agatgcagag
     4201 ccacttgctc cggggttcgc gtatattgct ccgggtggac gacatctgga agtttcgtcg
     4261 gatgggagcc aattgcgcat aacggatgga gatccggagc tgagctgtcg cccgtctgta
     4321 aacgttcttt ttcgatctgt cgcgcgagca tttggacccc aggcactcgg gttgattctg
     4381 acgggtatgg gagaggatgg atactcaggc attcaagaaa tacgcgctcg aggtggtaag
     4441 atcattgcgc agagtcagga ctcgtgtttg atttttggca tgcccgcgag acccgtacgc
     4501 gaaggactcg tagacgccgt actttctccg gataaaatcg cagaacgcct caatgaatta
     4561 tttgcgggag tgcgtacatg agtaattctc cccccaattt gacccgcatc aagacagtca
     4621 tccgcgcgaa gaccggtgtg atcattgatg acaagaacga atatttgatc ggaagccgtc
     4681 tgggaatcct actcgaagag aacaat
//
LOCUS       DAOQJL010000290         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10797_length_4704_cov_4.431060, whole genome shotgun sequence.
ACCESSION   DAOQJL010000290 DAOQJL010000000
VERSION     DAOQJL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10797_length_4704_cov_4.431060"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLG78_12420"
     CDS             complement(<1..1488)
                     /locus_tag="PLG78_12420"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNE23969.1"
                     /translation="MAEKTPAAELPIEEHVKVVHSRLLREKPAPYVPKGEDDPEVVTI
                     RPGAAKRAMFSGEDIVEIDLPVGSRVIYPKAPIRGLPDPDEAIRHALMHPEKMNPLPH
                     YLKPGMKVTICIDDISLPLPPMERPDMRERILHIVLEYLDNAGVTDVHLIVATAFHRR
                     MTDGEIRRMVGPEIFKRFHPDRLYNHDAENKEGMKWLGHAEKGEAVEINRRAAESDLI
                     IYVNINFVPMNGGHKSVATGLAGYKTLREHHDPEVIANCNSYMDPDGSELARSNKRVG
                     SFIEARIPIFHIESALNNRMFKGPLQFLSKNEDDFNAVEKRAMTALKQSLKRMTPDIK
                     RKIFQRVYAPYEPIAVYAGQVDSVHDRILRASRRQYTVRIRGQADVLIMGIPFVSPYS
                     VNSILNPLLVQVMALGYLYHLYEGGVPLLRDGGTLILFHPCRDEFDPKHHPSYIEFFH
                     RILPEGTDSLTLQKKYEREFATNPDYIKMYREGNAYHGVHPFYMWY"
     gene            complement(1508..2176)
                     /locus_tag="PLG78_12425"
     CDS             complement(1508..2176)
                     /locus_tag="PLG78_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNE23970.1"
                     /translation="MKEKAAVFYDVDGTLVHTNILHVYVYYAMRLPKISEKARKVLLM
                     LLFTPLYMLIDQMSRTVFNKLFYRNYKGMPEERLRILGKELVREGLMPNVYRDAMERI
                     KKAKKMKLTQVLVTGSLDFAMKQFAKELGIDHVIANQLEMKAGIATGVLVPPILAGRE
                     KLKAMKDFAARENVDLSRSYAFADSKYDLEMLEAVGYPVAVNPDAGLRKIAESRGWPI
                     VSFT"
     gene            complement(2173..2550)
                     /locus_tag="PLG78_12430"
     CDS             complement(2173..2550)
                     /locus_tag="PLG78_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE23971.1"
                     /translation="MAEEFHYPAPIDVGEAPTPGKSILVELPNPPSGYAKDEAFVFTL
                     EDGQVVGYFNRCSHVQVPLDMGDGDFLDRSGFIMCRVHGARYDLVTGDALLGPAVRGL
                     TRLELALRDGRIIVTGWKKGRQA"
     gene            complement(2691..3536)
                     /locus_tag="PLG78_12435"
     CDS             complement(2691..3536)
                     /locus_tag="PLG78_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23972.1"
                     /translation="MRKLVLLGALLLAGSAEAATYRLFVHGRSGDNHCQPITSTASAT
                     NDHNNYWGGTVTGLANVRYVGFDGTQNGGAYSWNSCGAQKQLNDAVRIFCTGGNTCEI
                     YTHSTGGLVAAAYFGLNNPSGLSISRIQLLASAAGGSELADISTTYLSWLGIKTLGGQ
                     LDQSVSTRGARNGFNHNNAGGRTFYTTSGEGSDYWNLTSPFLPGKDDGVLANHSLCSV
                     NSVTDVGVSCTRGSAAMSESYRCGFLWLSTCHKSYGRWTPYYTVYTGGSNHSHGDAKA
                     DYNRR"
     gene            complement(3614..>4704)
                     /locus_tag="PLG78_12440"
     CDS             complement(3614..>4704)
                     /locus_tag="PLG78_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23973.1"
                     /translation="SHKDPTISPVGPEGIPIVNEDPREVLERYVKWAQYPPTSRPLHA
                     GMVDLTDPYNMDRPPVGVVAKPAQQCTSVDGIPRCEKPAVFSDVQCKMTPQASISVGK
                     GDFKITLKCFNKEGVNQPLNGVVPKVYRNLFRKDYPSLPPVSFGDRGTDGDEKAGDHE
                     WTFIVRPTQQDWGDMFLEVDFEVAGFKHNQRTSWFSTPHTVAEFRDGIQDSVRNGHLI
                     VSVPVVITKKGYYSFDANLQGTTGDKAFAATSSVEGDFEAGARTIDFQFWGKVLRDSG
                     VDGPYVVREIRGRRNNSPVTPSMVRKALAENREISGNHTEPLWEYMNMAPNYTTKSYS
                     ADDFSKEEWNSEEKNQRIEFLKKQVEQE"
BASE COUNT         1142 a         1214 c         1103 g         1245 t
ORIGIN      
        1 gtaccacata tagaacggat gaactccgtg atacgcattg ccttcgcgat acatcttgat
       61 gtagtctgga ttcgtggcga attctctttc gtacttcttc tgcagtgtga gcgagtctgt
      121 accttcgggc agaattctat ggaagaattc aatgtaactc ggatgatgct ttggatcgaa
      181 ttcgtctctg catggatgaa agaggatcag agtacctccg tcacgcagaa gtggaactcc
      241 tccttcataa agatgataga gataccccag cgccatcacc tgaacaagga gtggattgag
      301 aatcgagttc actgagtacg gactcacaaa tggaattccc atgatgagca catccgcctg
      361 cccgcgaatt cgcacagtgt actgcctgcg actcgcacgt aaaatgcggt cgtgcactga
      421 atccacctga cccgcataaa ccgcgatcgg ctcataagga gcatacactc tctgaaatat
      481 ctttcgctta atgtccggag tcatcctttt gagactctgc ttgagagcag tcatggccct
      541 cttttccact gcgttgaagt cgtcttcgtt cttcgaaaga aattgaagag gacccttgaa
      601 cattctgttg ttgagagcag attcaatatg aaagatcggg attcttgcct cgatgaacga
      661 ccctactctt ttgttggagc gagccagttc agaaccgtcc ggatccatat aggaattgca
      721 attcgcgatt acttccggat catgatgttc gcgcagagtt ttataaccgg caagacccgt
      781 cgcaacggac ttgtgtcctc cgttcattgg cacaaaattg atgttcacgt aaatgatcag
      841 gtcactttcc gcagcgcgtc gattgatttc taccgcttct cctttttcag cgtggcccag
      901 ccatttcatt ccttctttgt tttccgcgtc atgattgtag agacgatccg gatgaaagcg
      961 tttgaagatc tcagggccca ccattctacg aatctctcca tccgtcatgc gacgatgaaa
     1021 cgcagtggcc acgatcaaat gaacatctgt tacgccagcg ttgtccagat actcaagaac
     1081 gatatggaga attctctctc gcatatccgg gcgttccatg ggcggtaggg ggagagaaat
     1141 atcatcgatg cagatggtca ctttcatccc tggtttcaaa taatgaggaa gaggattcat
     1201 cttttcggga tgcatcaagg catggcgaat cgcttcgtct ggatctggaa gaccgcggat
     1261 cggggctttg ggataaatga cgcgtgaacc tacgggtaga tcgatttcta caatatcctc
     1321 tccggagaac atcgccctct ttgccgcacc cggccgaatg gtgactactt caggatcgtc
     1381 ttcacctttg ggtacgtaag gtgccggctt ctcgcggagt agacgcgaat gcactacttt
     1441 tacatgttct tctataggta gttcagctgc gggtgttttt tctgccatgt tcttcctgct
     1501 taaaaaacta agtaaaactt acgattggcc atccgcggga ttctgcgatc ttgcgcagcc
     1561 ccgcgtccgg gtttaccgca accggatatc cgactgcttc tagcatttcg agatcatatt
     1621 tggagtccgc aaacgcatag gagcgcgaca gatccacatt ttcgcgcgcg gcaaaatcct
     1681 tcattgcttt gagtttttcg cgcccggcaa gaatcggagg cacgagcact cctgtggcaa
     1741 ttcctgcctt catttccagc tgatttgcaa tgacatggtc tatcccaagc tcttttgcaa
     1801 attgcttcat tgcaaaatca agagagcctg taaccagaac ctgagttagt ttcatttttt
     1861 tagctttctt gattctttcc attgcatcgc gatacacgtt tggcataagc ccttcgcgca
     1921 caagttcctt gcccagaatg cgaagccttt cttccggcat tcctttgtag tttctataga
     1981 agagtttatt aaaaactgtt ctactcatct gatcgatgag catatagagc ggcgtaaaaa
     2041 ggagcatcag cagtactttt cgcgcctttt cagaaatctt gggcaacctc attgcatagt
     2101 atacatacac gtgcaaaata ttggtgtgaa cgagtgtgcc gtccacatcg taaaatacag
     2161 ctgccttttc cttcatgcct gtcttccttt cttccatcca gttacgataa tacgcccgtc
     2221 acgaagtgcc agttccagtc ttgtcagccc tctcacggca gggccaagca aagcgtctcc
     2281 tgtgaccagg tcataccggg caccgtggac tctgcacata atgaaaccgc ttctatccaa
     2341 aaaatctccg tcgcccatgt ccagtggaac ctgcacatgc gaacaccgat tgaaatatcc
     2401 tactacctgt ccgtcttcga gggtgaagac gaatgcttca tcctttgcat aaccagacgg
     2461 aggattcgga agctccacca gaatggattt gccaggcgta ggcgcctcac ctacgtcaat
     2521 cggagccggg tagtgaaact cttcggccat tcgaccagaa gcccaaaaag gcctatttac
     2581 gaaatgaaaa aagcccctct agaaaaacag accgttagag agtgacacaa ggtagaaaca
     2641 aaaaaagccg gcaccaggcc ggctttccag acttacaatc aaagatccaa tcagcgacga
     2701 ttgtaatccg cttttgcatc tccgtgtgag tgattgctgc caccggtgta tacagtgtag
     2761 tatggtgtcc agcggccgta agatttatgg caagtgctca gccacaggaa tccacaacga
     2821 tagctttcgc tcatagctgc acttccccga gtacaggaaa ctccgacatc tgttactgag
     2881 ttcacgctgc agaggctgtg attggcaagt acgccgtcat cttttcctgg aaggaaagga
     2941 ctggtgagat tccagtagtc agaaccttcg ccggaagtgg tgtagaatgt acgaccacct
     3001 gcgttgttgt ggttaaaacc atttcttgca ccgcgtgtgc tcacagactg atcgagctga
     3061 ccgccgagag tcttgatgcc cagccaggaa aggtaagtcg tagaaatgtc cgctagttca
     3121 gatccacctg ctgcagacgc cagcagttga attctgctga tgctgagtcc agacggattg
     3181 ttcaaaccaa aataagcagc agcgaccagg ccgcctgtag agtgagtgta gatttcgcat
     3241 gtgtttccgc ctgtgcagaa aatgcgaacc gcgtcattca actgcttctg cgctccgcag
     3301 gaattccagc tgtatgctcc gccattttga gtcccatcaa aaccaacata acgaacgttg
     3361 gccagaccag tgactgttcc accccagtag ttgttgtggt cgtttgtggc gctggcagtg
     3421 cttgtgattg gctgacagtg attgtccccg gatctaccgt gaacaaagag tctataagtg
     3481 gcagcttctg cgctgcctgc caggagcaat gctcccagaa gtacgagttt tctcatctta
     3541 gatatccccc tatctaacaa tgctcagaat gccacggcac tctggccgtg acaactactt
     3601 tttcagactc gagttattcc tgctccactt gcttctttaa gaactcaatt ctctgattct
     3661 tctcttctga gttccattct tccttggaga agtcatctgc ggagtagctc ttcgtcgtat
     3721 aattaggagc catgttcatg tattcccaga gcggttctgt atggtttcct gaaatctccc
     3781 gattttctgc cagggctttg cgcaccatgg aaggagtcac tggagaattg ttgcgacgtc
     3841 ctctgatctc gcgaacgacg tagggaccat ccactccgga atcgcggaga acttttcccc
     3901 agaattgaaa atcaatcgtg cgggctcccg cctcaaaatc tccttctaca gaagaggtag
     3961 cggcaaacgc tttatccccc gtggttccct gaaggttcgc atcaaagctg tagtagcctt
     4021 tctttgtaat tacaactggc acagaaacaa tcagatgacc gttgcgaacg gagtcctgaa
     4081 ttccgtctct gaactctgcc acagtgtgag gtgtactgaa ccagctggtg cgctgattgt
     4141 gtttgaaacc agcgacttcg aaatccactt cgagaaacat atcgccccaa tcttgctgag
     4201 taggacgaac gatgaacgtc cattcatggt cccctgcttt ttcgtctcca tctgtgccgc
     4261 gatctccgaa tgaaaccgga ggcagagatg gatagtcttt gcggaataga ttgcgataga
     4321 ctttgggaac aacgccgttt aacggctgat tcacgccttc tttattgaag catttcagag
     4381 tgatcttgaa atcacccttc cctacagaga tgctggcctg tggggtcatc ttacactgaa
     4441 catctgagaa caccgctggc ttttcacatc ttggaattcc atctacagaa gtacactgtt
     4501 gtgcaggctt tgcaaccact ccgactggcg gacgatccat gttgtaagga tccgtgagat
     4561 ccaccatacc tgcgtgcaga ggacgagacg taggcggata ctgagcccat ttcacgtagc
     4621 gctccagcac ttcgcgtgga tcttcattta caataggaat gccttccggg ccaaccgggc
     4681 tgatcgtagg atccttgtgc gaag
//
LOCUS       DAOQJL010000291         4703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10802_length_4703_cov_4.545181, whole genome shotgun sequence.
ACCESSION   DAOQJL010000291 DAOQJL010000000
VERSION     DAOQJL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10802_length_4703_cov_4.545181"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLG78_12445"
     CDS             <1..941
                     /locus_tag="PLG78_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNE23974.1"
                     /translation="VTVFEKNDRAGGLLRYGIPDFKMEKWLIDRRLEQMRIEGVTFKL
                     GVNVGVDISAEELIATFDAVVLALGCEVPRDLPLPGRQLKGIHFAMDFLRRQNKINAG
                     DQVPDAISAKGKNVIVIGGGDTGSDCVGTSNRHGAKSVRQIEIFDEPPKTRPSSTPWP
                     TYPRILRTSSSHEEGCIREWAVSTKEFKGNANGEVATLHGCKAKFEGGKFVDIPGSEF
                     VWEADLVLLAMGFVNPVQDGLIAQLTKMGMALDERKNVKATFGLEDGTFATTLPNVYA
                     CGDVRRGQSLIVWAISEGRKCAAQVHRNLLAKLKTV"
     gene            complement(997..2268)
                     /locus_tag="PLG78_12450"
     CDS             complement(997..2268)
                     /locus_tag="PLG78_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNE23975.1"
                     /translation="MEESATSVIIEQLQKLSGSRREDFTEDTIIGSDINQIRHTFYFL
                     RSERIPVIISYMGETYQARVERVENDRIILNAPGFSEGTARRCRLKFEIINILYQFEV
                     PIVDLSRDKIVVRMPAFVQSARHRKYVRVYPQDLFMRFNLVYMPMFGRRGVGQIVENR
                     YPKIVRELERDRPDLGLLVRIVTEAINEISPDYEFVMYNPDVKRTFLERVMMSEKKTL
                     FVRDTSRQDTYYSPLGIFGITNFEKEFRRIAREESIEEAENFFEQYRRAELVDFIANY
                     ICTPIILLDRVVGHIKIYSTVLDKRNLNPDEAQRIDLLVQLLNYALSKHAIAETYFTL
                     HNTKVINISMSGLLFELQDSNVFNYLLDHDRLKICIPIKAHEVELRAEITRLLPAENG
                     YRIGVMFFSGAPDDFHFLEGFIHDRIKAMLR"
     gene            complement(2317..2643)
                     /locus_tag="PLG78_12455"
     CDS             complement(2317..2643)
                     /locus_tag="PLG78_12455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE23976.1"
                     /translation="MITAEPRNNTLILHVTEPRVDMVVARKFREGLTAYLKDKPHRIV
                     LDLKETEYFDSSALGALVAFMKDVKAYGGQLVLCNLSRSLLTLLKLSKLDLLFEISED
                     LEKSIV"
     gene            2729..4534
                     /locus_tag="PLG78_12460"
     CDS             2729..4534
                     /locus_tag="PLG78_12460"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNE23977.1"
                     /translation="MPESSKHAIPEAVRARILDWAGPSFPLEIREEAVRVHADIMAGK
                     HSEEVEAYSGELEFGTGGLRGVLGHGAGRMNRYTVGRATLALCRVFAKRSRKPALVIA
                     YDSRRKSDEFSIVTAGIAAAQGFKVYLFDDVAPTPILSYAVRRLQAASGVVITASHNP
                     PEYNGYKVYAEDGSQIVGSIQKEIEKEIAAIAWSDLKFLSESDPLFKKQVKKIGPEIR
                     KAYFAEIARSPFATSARPEKKKLHLVYSPLHGTGGRWLPDLLRHFGFQITVVPEQEKP
                     NGEFPTVKYPNPEEPDALKLSRELAIQVDADLFLATDPDADRLGVGVRIERGNYALLN
                     GNQIGSLLCAHLCEKISQGKDASRYHVYKTIVTTDLQRRIADQNGIKIHDVLTGFKYI
                     AEQMGLLDTGSKQMGYKKGRDLYLFGGEESYGYLPVGFVRDKDSLSSALLLCEILAQR
                     GDLLGYLNQVYLKYGLYLEDLKSVTMKGLDGQKKIKEIMQRLRTDDWSGMQVGGRKVS
                     AVLDFLHQTRNGKKSHADFGVLPPSDVIQMVLEPEGRLTIRPSGTEPKVKLYISLKYP
                     GSPSSLDELAKARTELENELASASGIFIARTGLAG"
BASE COUNT         1225 a         1072 c         1253 g         1153 t
ORIGIN      
        1 aagttactgt atttgaaaag aacgatcgtg cgggtggact cctccgctac ggcattccag
       61 atttcaaaat ggaaaaatgg ctcattgatc gtcgtctgga gcagatgcga attgaaggcg
      121 ttacgttcaa gctgggtgta aacgttggag tagatatttc agcagaagaa ctcattgcaa
      181 catttgacgc agtggttcta gctcttggct gcgaagtacc tcgtgatctt cctcttcctg
      241 gccgtcagct gaaaggaatt catttcgcaa tggatttcct gcgcaggcaa aacaagatca
      301 acgcaggcga tcaagtacca gatgcgattt ccgcaaaagg caagaatgta attgtgattg
      361 ggggcgggga cactggttca gactgcgtgg gaacctcgaa tcgtcatggc gcgaagtccg
      421 ttcgtcaaat cgagatcttt gatgaacctc ctaaaacacg tccttcctct actccgtggc
      481 caacgtatcc gcgaatcctt cgcacgtctt cgagtcacga agaaggatgc atccgcgaat
      541 gggcggtgag tacaaaggaa ttcaaaggca acgcgaacgg agaagtggcc actcttcatg
      601 gatgcaaggc aaagttcgaa ggcggcaagt tcgtagatat tcctggaagt gaatttgtat
      661 gggaggcaga tctggttcta ctcgccatgg gttttgtaaa tccagtccag gacggcctga
      721 tcgcgcagct cactaagatg ggcatggctc tggacgaacg taaaaatgtg aaagctacat
      781 ttggactcga agacggaacg tttgccacga ctcttcccaa tgtttacgca tgcggagacg
      841 ttcgtcgcgg gcagtctttg attgtctggg ctatctctga aggccgtaaa tgtgcggccc
      901 aggttcaccg caacctgctt gcgaagctga aaactgtttg atggaaaagc cgcatttgcg
      961 gcttgtttcc gcaccatcta tcaggtgcgc aaacgcttag cgaagcatcg ctttgatgcg
     1021 atcgtgaatg aacccttcga gaaaatgaaa gtcgtccgga gcgcctgaaa agaacatcac
     1081 tccaatcctg taaccattct ccgcaggcaa aagcctggta atttcggcgc gcagttcaac
     1141 ttcgtgcgct ttgatcggga tgcagatctt caatcgatca tgatcgagca gatagttgaa
     1201 tacgttgctg tcctgaagct caaagagcag accactcatg cttatgttga tgaccttcgt
     1261 attgtgcaga gtaaaatatg tttctgcaat cgcatgtttg ctgagtgcat agttcaatag
     1321 ttgaaccagt agatcgattc tttgagcttc atccgggttg agatttctct tgtccagtac
     1381 tgtggaatag atcttgatat gccccacgac tcgatcaagt agaatgatgg gcgtgcatat
     1441 gtaatttgca atgaaatcga ccagttctgc gcggcgatat tgctcaaaga aattttctgc
     1501 ttcttcaatg gattcttcgc gggcaattct tcggaattcc ttttcaaagt ttgtaatgcc
     1561 aaagatcccg agtggcgaat agtacgtatc ctgtctcgat gtatcgcgca caaacagagt
     1621 ctttttttcg ctcatcatga ctcgttcgag aaaagttcgt tttacgtctg gattgtacat
     1681 gacgaattca taatcaggac tgatttcgtt gattgcctcc gttacaattc tcaccagcaa
     1741 accaagatcc ggcctgtctc tttcgagctc gcgcacaatc ttgggatagc gattttccac
     1801 gatctgaccc acaccgcgtc ttccgaacat gggcatgtac accagattga atcgcatgaa
     1861 caaatcctgc gggtaaacac gaacatactt ccggtgtcgc gcagactgaa cgaaagccgg
     1921 catgcgcact actatcttgt cgcgagaaag atccacaatg gggacttcga actggtaaag
     1981 aatatttatg atttcgaact tgagtctaca tctacgcgca gttccctcgg agaatcctgg
     2041 ggcattcaga atgattcgat cgttttcaac tcgttctacc cttgcctggt aggtttcgcc
     2101 catgtaggag atgatgactg gaatgcgctc ggagcgaagg aaatagaaag tatggcgaat
     2161 ctgatttatg tcagatccga tgatcgtatc ttcagtgaag tcctcacgtc gcgatccaga
     2221 gagtttctga agctgctcga tgatgacgga agtagcgctt tcttccactt atagaaaatc
     2281 gacctctgga gaggtgaaat tgcgggtgtc tggactttaa acgatgcttt tttcgagatc
     2341 ttcgctgatt tcgaaaagga gatccagctt ggaaagcttg agcagagtca ggagggatct
     2401 ggaaagattg caaagaacca gctggccgcc gtatgctttt acgtctttca tgaaagcaac
     2461 gagtgcacca agagcggaac tgtcaaagta ctctgtctct ttgagatcga gcacgatgcg
     2521 atgcggcttg tccttgaggt aggccgtgag tccctctcgg aacttgcgtg ccaccaccat
     2581 gtctacacga ggttcggtta catggaggat cagagtatta ttgcgcggct cggcggtaat
     2641 catggcccag aaaaagaaaa attgacccac actcccctgc ggtcaaataa aaatttactc
     2701 cagggtcccc gggaggaaaa gttcctacat gccagaaagc tccaaacatg ccattcctga
     2761 agccgtgcgg gccagaatcc tggactgggc aggtccctct tttccgctgg aaatcaggga
     2821 agaggccgtc cgcgtccacg cggacatcat ggcgggcaag cattcggagg aggtagaggc
     2881 ctactccgga gagctcgaat tcggcacagg aggcctgcgg ggcgttctgg ggcatggtgc
     2941 gggccggatg aatcgttaca cggtgggtcg ggccactctg gctctctgtc gcgtatttgc
     3001 aaagagatcg cgcaagccag ccctggtgat cgcctatgat tcccgtagaa agagcgatga
     3061 gttttccatt gtgacggccg ggattgcggc cgctcaagga ttcaaggtct acctttttga
     3121 tgatgtggcg cctacgccta ttttatctta tgcggttcgc agacttcagg ctgcttctgg
     3181 agtagtgatc accgccagtc acaatcctcc ggaatacaat ggatacaaag tgtatgcaga
     3241 ggatggatct cagattgtag gatccattca gaaagagatt gaaaaagaaa ttgctgcgat
     3301 cgcctggtcc gatctgaagt ttttgagcga atcggatcca ctgtttaaga agcaagtgaa
     3361 gaagattggt cctgagattc gcaaggctta ctttgcggag attgctcgct caccgtttgc
     3421 aaccagcgcg cgcccagaaa agaagaagct tcatctggta tactctcctc tccacggaac
     3481 aggaggcagg tggttgccgg accttctgcg tcatttcgga tttcaaatca cagtagttcc
     3541 ggagcaagaa aagccaaacg gggaatttcc gaccgtgaag tatccaaacc cggaggagcc
     3601 agacgctctc aagctctcga gagaattggc cattcaggtg gatgcggatt tgtttctggc
     3661 aacagacccg gatgcggatc ggcttggtgt aggcgtgcga attgagagag gcaactacgc
     3721 cctgttaaac ggaaatcaaa tcggaagctt gctctgtgcg cacctttgcg aaaagatttc
     3781 tcagggaaaa gacgcatcgc gttatcacgt ttacaaaaca attgtgacca cggacctgca
     3841 gaggcgcatt gcagatcaaa acggaatcaa gattcacgat gtcctgactg gtttcaaata
     3901 catcgcagaa caaatgggtc tcctggatac cggtagcaaa caaatgggct ataagaaagg
     3961 ccgtgatttg tatctgtttg gcggagaaga atcgtacgga tatctgcctg ttggttttgt
     4021 gcgggacaaa gactcactat ccagtgcgct cttgctttgc gaaattctgg cgcagcgcgg
     4081 ggatcttctc gggtatttga atcaggttta tctcaagtat ggtctttacc tggaagattt
     4141 gaaatccgtc acgatgaagg gactggatgg tcagaagaag atcaaagaaa tcatgcaacg
     4201 ccttcgtacg gacgattggt ctggaatgca ggtgggcggt cgcaaggtaa gtgccgttct
     4261 tgattttctc caccagacca ggaatggaaa gaagtcgcac gctgattttg gagtacttcc
     4321 gccatcagat gtaattcaaa tggttcttga accagaagga agacttacga ttcgtccatc
     4381 cggcacggag ccaaaagtta agttgtatat aagtctaaag tatccggggt cgccatccag
     4441 tctggacgag cttgcgaaag cacgaacaga attggaaaat gaactggcaa gcgcatcggg
     4501 gatcttcatc gcacgcactg gacttgcggg ataagacgga acaatcgagg tagagaatgg
     4561 actggaaagt agaagaaacg gaaggccagc ggactgcaag actgagtgct gcggcggaag
     4621 gaagagacct ggcaccggga gaaagaatgg agcgcatctt cgcgcagcca atgtctttcg
     4681 ctgacattaa ggaaaaatcg gat
//
LOCUS       DAOQJL010000292         4702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10808_length_4702_cov_4.012912, whole genome shotgun sequence.
ACCESSION   DAOQJL010000292 DAOQJL010000000
VERSION     DAOQJL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10808_length_4702_cov_4.012912"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLG78_12465"
     CDS             complement(<1..571)
                     /locus_tag="PLG78_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE23978.1"
                     /translation="MSSFTATKWRFRLALGFLIFAAAAAWAASYYHIAYIFSDSARGN
                     EDVAKLLTLVDILSGSLLAALLFAAVFIFRPLVLGLGRQHRILESLESRSRAVVESAP
                     DGILAFDEKGIIESFNPAAEKLFACSASEVIGKSIQTIIPGYRGSLEVNAETSASRIT
                     GIERQIVGHRWDGSTFDMDLTVSELMVDDK"
     gene            complement(604..2205)
                     /locus_tag="PLG78_12470"
     CDS             complement(604..2205)
                     /locus_tag="PLG78_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YndJ family transporter"
                     /protein_id="HNE23979.1"
                     /translation="MELRIPGIEEWILLFHLFFLLPGIIGLTFIEGKSRYPFRIAACS
                     TALAMFLKPGLASAALTIPTIAGSMVSFAHGFRLLGLRAFADRGRHRLSHACMAAGLM
                     YLPVGTGFLFAFRAGLNTGYSDAITSLTSVHFHYAAFSTSILAGFITRATDESTWPRF
                     QPLLLLGVMTGPAVTGIGIALGSRVELFASLYMTVFASALAFTWIFRVTFNGAGVRRS
                     AGVNHPNRLRAYLGVSSICLLMAMALASTYAIRPDLGAFPWIYWMIRTHGILNSAGFV
                     VPATIAALYCLGPLPPFQAVLSRLTGKLTIGKDSLNERFLEQTPGLLSLDDFASPQFS
                     PRSVHATIRDFYLRTSEYTMTVQSRWLPGFRLAGRLFKSFSRKIGQINFPEHKVRRQT
                     HGRLFRASPDNRGELTAWVRTYVESPDQKTESCLYAALYAKMPSSLVNIAMIMPGGNL
                     TSILRFSNDAHDGLILSSIPEAEPDTGIYFALFGRLALRLPMDESFHLVADNEHIRAL
                     HSMELFGYTFLTLDFEIAKANLRSA"
     gene            2292..3608
                     /locus_tag="PLG78_12475"
     CDS             2292..3608
                     /locus_tag="PLG78_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE23980.1"
                     /translation="MIIHPESKLKEFWDLYMLVLTVACALIIPLQIALHVPFHSSLLW
                     FESLLSISFGVDIVLHFFTGFRFENRIILDPRRIAVHYLRSWFILDLIAALPFFLIHG
                     TDAAEATQLLRVIRFLQIHRLLKLLRVNEVFSQFQRKHALNPGLFRLGYFVLLISLIT
                     HMLACGWILLDGITDETDPVTIYIKALYFTVTTLASVGYGDITPHNNIQRIFALGLMM
                     IGVGSYGFVIGNMASFLANRDIVRANYFKKLEEVTAFLRYRAIPSDLRSQILAYYDHV
                     WESRMGQDEAELLSDLPQSLRVDIALAMRKDLIQKVPFFREASEDLLRDIVMALRPAA
                     YIPGSFIIREGEMGDCMYIVSSGIVEIVSRDGSQVFTELTEGSFLGEMALIYKTERTA
                     SARARGYCDLYVLKKRDFDFILSRYPAFASHIMKVAEERRREAASR"
     gene            complement(3660..>4702)
                     /gene="glnA"
                     /locus_tag="PLG78_12480"
     CDS             complement(3660..>4702)
                     /gene="glnA"
                     /locus_tag="PLG78_12480"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE23981.1"
                     /translation="FGPENEFFIFDDLKIKDEINAQSYEIDSTEGVWRSNSDSEGSIN
                     TGHRPYTKGGYFPVAPVDSQVDIRAEIVKTLHKIGMETFVVHHEVAQGQGEIGVKFGT
                     LIEAADNVQKLKYVVKMVAHRHGKTATFMPKPLYGDNGNGMHTHQSVWKDGKPLFAGS
                     GYQNLSDVAMNYIGGVLKNGKSIAAFTNASTNSYKRLMPGFEAPSILAYSAQNRSASA
                     RIPFVNSDKARRVEFRFPDSSANPYLAFASMLMAGIDGIKKKIDPGPPHEEDLFELST
                     DEIKAKGINLMPYTLRDALTSLEGSSDFLRAGDVFTDHFLKTYIQYKYDTEVTPYEGR
                     PHPIEFVNTYSC"
BASE COUNT         1141 a         1120 c         1260 g         1181 t
ORIGIN      
        1 ttttgtcgtc caccatgagt tcacttacgg taagatccat gtcaaatgtg gatccatccc
       61 atctgtgtcc cacgatttgc ctttctatgc cggtgatgcg tgatgcagat gtttccgcat
      121 ttacttcgag ggagcctctg taacccggga tgatggtctg gatgcttttt ccgatcactt
      181 ctgatgcact gcatgcaaac aatttctctg ccgcaggatt gaaagattcg atgatccctt
      241 tttcgtcgaa tgccagaatt ccgtccggtg cactctctac cacagctctg gaccttgatt
      301 ccagactttc cagaattcta tgctgacggc ccaggccaag aaccagtggg cgaaagataa
      361 aaacggccgc gaacaaaagt gctgcgagaa gggatccaga aagaatatca acgagggtga
      421 gaagttttgc aacgtcttca ttgcctcgtg ctgaatcaga gaagatgtac gctatgtgat
      481 aatagctggc ggcccatgcg gctgcggcag caaagatgag aaatcccagg gcgaggcgaa
      541 atcgccattt ggttgcagtg aaagaagaca tctccctgga gaatggagta gaagagctgc
      601 gcgtcaagcg gatctgagat ttgcttttgc gatctcaaaa tccagagtca ggaatgtgta
      661 cccgaatagt tccatggaat gcagtgcccg aatgtgctcg ttgtccgcca ccaggtggaa
      721 cgattcgtcc atgggcaggc gaagggcaag gcgcccgaac aaagcgaaat agatgcctgt
      781 gtccggttca gcttcgggga tagaagacag gatgagtccg tcgtgcgcgt cattggaaaa
      841 gcgcagtatg ctggtgagat tgcctccggg catgatcatg gcgatgttta caagagagga
      901 cggcatcttc gcgtagagtg ccgcataaag acatgattct gttttttgat ctggcgattc
      961 aacatacgtt ctgacccagg cagtcagttc gccacggttg tccggactgg cgcgaaagag
     1021 cctcccgtgt gtttgcctgc gcactttgtg ttccggaaaa ttgatctgtc caatcttgcg
     1081 gctgaagctc ttaaacagtc ttcccgcaag cctgaagccc ggaagccatc tcgattgaac
     1141 ggtcattgtg tactcggatg tgcgaaggta gaaatcgcgg atcgtagcat ggactgagcg
     1201 cggcgaaaat tgcggggatg cgaaatcatc cagcgagaga aggcctggcg tttgttccag
     1261 gaatcgctca ttgagagaat cctttccgat cgtgagtttt cccgttaggc gactcagcac
     1321 agcttgaaat ggaggcaagg gtcccaggca atagagagcc gcgatcgtgg ctggaacaac
     1381 gaatcccgcg gagtttagaa ttccatgggt gcgaatcatc cagtagatcc agggaaatgc
     1441 ccccaggtct gggcgaatcg cgtaggtaga agcaagtgcc atggccataa gcaagcaaat
     1501 ggaagagacg cccagatatg cgcgaagacg attgggatgg tttactccgg ccgatcttcg
     1561 cacgcccgct ccgttgaaag tcacgcggaa aatccaggtg aatgccaggg cggatgcgaa
     1621 gaccgtcatg tagagagaag caaacagttc cacgcgggaa ccaagagcaa ttccaattcc
     1681 agtcactgcc ggtccagtca tcactcctag aagcagcaga ggttgaaagc gaggccaggt
     1741 gctctcatcc gtcgcgcgcg tgatgaagcc agcaagaatg ctcgtgctaa aggcagcgta
     1801 gtgaaaatga accgaagtca gtgaagtgat cgcatccgag tagccggtat tcagtcctgc
     1861 gcgaaatgcg aaaagaaagc ctgtccccac cggtagatac atcaacccgg cggccatgca
     1921 tgcgtggctc aggcgatggc gacccctatc cgcgaaggcc ctcaggccca gcaggcgaaa
     1981 gccatgcgcg aaagagacca ttgatcctgc aatcgttgga atggtgagag ccgcggacgc
     2041 gagtccaggt ttgagaaaca tggcaagagc agtcgagcac gccgcgatcc gaaagggata
     2101 tctggatttc ccctctatga aagtaagtcc aataattcca gggagaagaa agaaaagatg
     2161 gaacagcagg atccattctt ctatgcccgg gatgcgaagt tccatacgaa tcaggttgcc
     2221 agcctgattt ccgtcggcag ggttgtccag acatcgacga atcgttcgaa tggttcgaag
     2281 gcagaacaaa cgtgatcatt catcctgaga gcaaactcaa agagttctgg gatctttaca
     2341 tgctggttct caccgttgca tgtgcactga tcattcctct ccagattgcg ctgcatgttc
     2401 cgtttcactc atccctgttg tggtttgagt ctttgctttc gatttctttt ggagttgata
     2461 ttgttcttca tttttttaca ggctttcgat ttgaaaatag gatcattctg gaccccaggc
     2521 gcatagcggt tcattacctt cgttcctggt tcattctgga tttgattgcg gctcttccgt
     2581 tcttcttgat ccacggaaca gacgcggcgg aagcaacaca gcttctgcga gtgattcgat
     2641 ttttgcagat tcatcgtctg ttgaaattgc ttcgggtcaa tgaagtgttc tctcaattcc
     2701 agagaaagca tgcactcaat cccggcttgt ttcgtcttgg atactttgta ctgttgattt
     2761 ctctgatcac tcacatgctg gcatgcggtt ggatcttgct cgatggaatc acggatgaaa
     2821 cagatccagt caccatttac attaaagctc tctactttac tgtgaccacg cttgccagcg
     2881 tgggttacgg agacattacc ccacacaaca acatacagag gatcttcgct ctgggcttga
     2941 tgatgatcgg agtgggaagc tacggctttg tgattgggaa catggccagc ttccttgcaa
     3001 atcgagacat tgtgcgcgca aactatttca agaagttaga agaagtcact gcgttcctca
     3061 gatatcgcgc catcccatct gatctgcgat ctcagattct tgcttactat gatcacgtct
     3121 gggaaagcag gatgggtcag gatgaagcag aattgctgag cgatcttcca cagtcgctgc
     3181 gagtggacat tgcactggcc atgcgaaagg acctgattca gaaggttcca ttcttcagag
     3241 aggcgtctga ggatttgctt cgcgacattg tcatggcact cagaccggca gcctacattc
     3301 caggatcctt catcatccgc gaaggtgaaa tgggagactg catgtacatt gtaagctctg
     3361 gtatagtgga gattgtatcg cgcgatggct ctcaggtatt cacagagctt acagaaggga
     3421 gttttctcgg ggaaatggca ctgatttata agacagagcg aaccgcaagt gcgcgagcac
     3481 gcggatactg tgatctttac gttctcaaga aaagggattt tgatttcatt ctatcccgtt
     3541 atccggcatt cgccagtcac atcatgaaag ttgcggaaga aagaaggaga gaagctgcca
     3601 gtcggtagtg aagaaagaaa tgaaggtgcc ggcgggagaa gtccccgccg gctcaagtgt
     3661 tagcaggagt aagtgtttac gaattcgatc ggatgtggtc tgccctcata gggtgttact
     3721 tccgtgtcgt atttgtattg aatgtacgtc tttaggaagt gatccgtaaa aacgtctcca
     3781 gctctcagga aatccgaaga tccttccagg cttgtgagag catcgcgcag tgtgtaaggc
     3841 atcagattga tcccttttgc tttgatctcg tctgtggaaa gttcaaagag gtcttcttcg
     3901 tgaggtggac ctggatcaat cttctttttg atgccgtcaa ttccagccat cagcatcgat
     3961 gcgaaagcca gatacgggtt tgcagaagaa tccgggaaac ggaactcaac gcggcgagcc
     4021 ttgtcgctat tcacgaatgg gattcttgca cttgcgctgc ggttctgcgc agagtaggca
     4081 aggatggaag gcgcttcgaa acctggcatc aatctcttgt aagagtttgt ggatgcgttg
     4141 gtgaaagcag cgatgctctt gccattcttc aacacgccgc cgatgtagtt catggccaca
     4201 tcgctcaggt tctggtatcc gctgcctgcg aagagaggct tgccgtcttt ccagactgac
     4261 tggtgagtgt gcatgccgtt tccattgtca ccatacagag gcttgggcat aaatgtagcg
     4321 gtcttgccat gtctgtgcgc aaccatcttc actacgtatt tcagcttctg aacattgtct
     4381 gcagcttcaa tcagggttcc gaattttaca ccgatctctc cctgtccttg cgcaacttcg
     4441 tggtgaacta caaacgtttc cattccgatc ttgtgcaggg tctttacgat ttcggcccgg
     4501 atgtccacct gtgagtccac aggggccaca ggaaaatatc cacctttcgt gtagggacgg
     4561 tgacctgtgt tgatgcttcc ttcgctgtcg gagttggatc tccagacacc ttccgtacta
     4621 tcaatctcgt aggattgagc gttgatctcg tctttgattt tgagatcatc aaagatgaag
     4681 aattcgttct ccggtccaaa at
//
LOCUS       DAOQJL010000293         4695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10830_length_4695_cov_3.913362, whole genome shotgun sequence.
ACCESSION   DAOQJL010000293 DAOQJL010000000
VERSION     DAOQJL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10830_length_4695_cov_3.913362"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLG78_12485"
     CDS             complement(<1..144)
                     /locus_tag="PLG78_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23982.1"
                     /translation="MALSQNLVQKQTQKLIITQDLRQSIELLPLSNLELSEKIEKEVL
                     ENPM"
     gene            complement(149..856)
                     /gene="lptB"
                     /locus_tag="PLG78_12490"
     CDS             complement(149..856)
                     /gene="lptB"
                     /locus_tag="PLG78_12490"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNE23983.1"
                     /translation="MEDLSKAYNKRRVVDGVSFEICTREIVGLLGPNGAGKTTSFYMA
                     VGLVAPDSGKIYIDNKDVTTMPMYLRAREGIGYLAQEASVFRKLTVAENIESVLETMN
                     LTRSEIVERRDRLIDELHLNKVTNQNGYTLSGGERRRCEIARALATEPDFILLDEPFA
                     GVDPIAVKDIQQLIGHLRERGLGILITDHNVRETLKITDRAYIMYAGEILKSGTAKEL
                     VNDPKIRQIYLGEDFTL"
     gene            complement(906..2363)
                     /locus_tag="PLG78_12495"
     CDS             complement(906..2363)
                     /locus_tag="PLG78_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23984.1"
                     /translation="MKRVAIIVAFLISLSASACRAGFIRQNPPVTEEQTNPQKIDPQT
                     EEKPEPQVEPKIENVNNPENIIEEKTQDSSGRLVRVQIYGDELIREEVEVNGIPVKRT
                     TLRGNAVVLHNGVRIQAPLIVIEGGVRGACTGGVVIADPANGMSIYAERGDYDRQAQT
                     VILSGFPRMVSEGKGRKPAVLTSGRMVRKMADSVSVLEGDVRIATEGWTIVGENAVFA
                     DKDKKIDLPGDPMIFGNGQFITGNTLSYFTEERKLVLHGDIVNIRREDRSIVADKKPP
                     TLEQYVRSGERDNGGLDISGPGTLTADEITHDFKDKENVKTIVRGNVLFTREGLRIAA
                     PSLESIGSSATHLIAREGVDSTDHKQNLHITAGKMVYERASRMLKLEQDPKIEFLKKD
                     SVEVTGSLSGALIERNFETKQTIARGNVRLVQSDVTAVGQKAVFQEDTGAVVIEGEPG
                     IEQEKGIIRCEKIVYYPEKKRLLLMNRIRGSLAKD"
     gene            complement(2360..2905)
                     /gene="lptC"
                     /locus_tag="PLG78_12500"
     CDS             complement(2360..2905)
                     /gene="lptC"
                     /locus_tag="PLG78_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNE23985.1"
                     /translation="MNRFLLLILLLAACQKDPLQDDSLEKGASIRLKNFSRKSFSIDG
                     KEEWDLQAKEAYIFRSGDKESRIVAYGLDFKQFDKGRRTGLIQAERGEIDYKKKVLNL
                     AGTVSFTDDKRTVQSEELKYTMEDKILESTASVLLVEGASTTHCRKGISVNRSTNVQV
                     CKSPAVTRVSRPGDKQGFEDL"
     gene            complement(2907..3746)
                     /gene="kdsA"
                     /locus_tag="PLG78_12505"
     CDS             complement(2907..3746)
                     /gene="kdsA"
                     /locus_tag="PLG78_12505"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNE23986.1"
                     /translation="MLIKEREFLAGKKIGGREPFFLIAGPCVIESRDMLKRVCETMKK
                     LSEELGILYIFKSSFDKANRSSHDSKRGPGIEEGLRDLENIRKEFGVPVLTDVHEREQ
                     VKAVSQVVDVLQIPAFLSRQTDLLQVCAESGKWVNVKKGQFMAPQDAAKIADKIKSCG
                     SDKYLVTERGVSFGYNNLVFDPRSPEIIHEMGIPFVFDGTHSTQLPGAGKESGGDRRF
                     APVLMRAAVASGLEGIFMEVHPDPPSAWSDSSNQYYLDQAPELLRGLYKIDRLVKTQI
                     FAG"
     gene            complement(3749..>4695)
                     /locus_tag="PLG78_12510"
     CDS             complement(3749..>4695)
                     /locus_tag="PLG78_12510"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE23987.1"
                     /translation="LKEKLAMFCNVPEGAIVSACDIATSIYEIPEMYKQGGLDLGVLR
                     HFGMKPGKLNFKKWAQILHVINHPRKTVRIAVCGKYIALQDAYRSVYESLMHAGIANQ
                     VTVEFVKIDPEDLERTRSTAALKGVHGILVPGGFGSRGIEGKLMSIHYARTKKIPFFG
                     ICLGMQCATIEFARNVLGLHGASSTEFDPKTHHPVISLLEEQQGIEQKGGTMRLGAYP
                     CVIKEGTLAAREYKTTRIRERHRHRFEFTLRYKEAMEKHGLTFSGTSPDGQLMEIIEL
                     KSHPWFVGVQFHPEFQSKPIACHPLFAGFVAAASKLAR"
BASE COUNT         1055 a         1264 c         1059 g         1317 t
ORIGIN      
        1 cattggattc tcgaggactt ccttttcaat cttctcagac aattcgagat tggacagggg
       61 aagcagttca atcgactgac gcagatcctg agtgatgatc agtttttgcg tctgcttttg
      121 aactaaattc tgcgaaagag ccataggttt aaagtgtgaa gtcctcgccc agatagatct
      181 ggcgaatttt cggatcgttc actagttcct tcgcagttcc agatttcaaa atttcaccgg
      241 catacataat gtatgctctg tctgtaatct tcaatgtttc gcgcacgttg tgatccgtaa
      301 tcaagattcc aagaccccgc tcgcgcaaat gaccgatgag ctgctggatg tctttgactg
      361 caatcgggtc cacgccagca aaaggctcgt cgagcaaaat gaagtcaggc tccgtggcca
      421 gagctcgtgc gatttcgcac ctgcgccttt ctccacctga aagagtgtac ccattctgat
      481 tggtgacctt gttcaagtga agctcatcaa tgagtcgatc ccttctttcc acgatctcgg
      541 atcgggtgag gttcatagtt tctaacacag attcaatatt ttctgccact gtcaattttc
      601 gaaacacaga agcttcctgc gccaggtatc caatcccttc gcgcgctcgc aggtacatgg
      661 gcatagtggt tacgtcttta ttgtcgatgt aaatttttcc gctgtccggt gccacgagac
      721 ccacagccat ataaaatgaa gttgtctttc ccgccccgtt cgggccgagg agtcctacga
      781 tttcgcgtgt gcagatttcg aaactcacgc catctacaac tcttcgcttg ttgtatgcct
      841 tggaaagatc ctccatgcga aagcgacgca ctggttcttc cgattgtttt ggtctgcgct
      901 gcacgctagt cttttgcaag agatcccctg atccgattca tgagtaaaag gcgcttcttc
      961 tctggatagt aaacgatttt ttcgcaacga atgattccct tctcctgctc gattccaggc
     1021 tctccttcga tcaccacagc accggtatct tcctgaaaaa ccgccttttg gcccaccgca
     1081 gtcacatcac tctgcaccag tcgaacgttt ccgcgtgcaa tcgtttgttt tgtttcaaaa
     1141 tttcgctcaa tcagggcgcc ggaaagggat cccgtcactt ccacactgtc tttcttcaga
     1201 aattctatct tgggatcctg ctcgagtttg agcatacggc tggcgcgctc gtagaccatt
     1261 ttgcctgctg tgatatgaag attctgcttg tgatccgtgg aatccacacc ctcccgtgct
     1321 atcaaatgag tggcagaact accgatggac tcaagagaag gtgcggcaat gcgaaggcct
     1381 tcgcgggtga ataacacgtt gcctcgcaca atcgttttta cgttttcttt gtccttgaaa
     1441 tcgtgggtga tttcgtctgc ggtcagagtg cccggcccag aaatatcgag acctccgttg
     1501 tccctctctc cgcttcgcac gtattgctcg agagtgggcg gtttcttgtc tgcgacaatg
     1561 gacctatctt cgcgccggat atttacaata tctccgtgca gtacgagctt gcgctcctct
     1621 gtaaaataac tgagagtatt tccagtgatg aactgaccat tcccaaagat cataggatct
     1681 ccgggtagat cgatcttctt atccttgtct gcgaaaactg cattctctcc tacaatggtc
     1741 cagccttctg tggcaatgcg cacgtcccct tctagaacag aaacactgtc cgccatcttg
     1801 cgcaccatcc ggccggaagt cagcactgcc ggttttcttc ctttgccttc actcaccatt
     1861 ctgggaaacc cggataggat cactgtctgt gcctgcctgt cgtagtctcc tctttctgcg
     1921 taaatggaca tcccgtttgc cggatctgca atgaccacac cgcccgtgca agcgcctcta
     1981 acaccacctt caattacgat gagaggggcc tgtatcctca ctccgttgtg gagcactaca
     2041 gcattgccgc gtaacgttgt tcttttgacg ggaatgccgt tgacttccac ttcttcgcga
     2101 atcagttcgt ctccgtagat ttgaactcgt accaaccttc cggaagaatc ctgagtcttc
     2161 tcttcgatga tattttccgg gttgttgacg ttttctattt ttggctctac ctggggctca
     2221 ggcttttcct ctgtctgtgg gtcaattttc tgaggattgg tctgttcctc ggtcactggc
     2281 gggttctgtc gaatgaatcc agcgcggcat gcagatgcag aaaggctgat gaggaatgca
     2341 acgatgatgg cgacgcgttt catagatctt cgaaaccttg cttatcgccc ggcctggaaa
     2401 cgcgagtcac tgcaggagat ttgcaaacct gaacattggt agaacgattc actgagattc
     2461 cttttctgca gtgcgtggtc gaggctcctt ctactagaag cacgcttgcg gtactttcga
     2521 gaatcttatc ttccatagtg tacttcagct cttccgattg caccgttctt ttatcatccg
     2581 taaagcttac tgtgccggcc aggttgagaa ctttcttttt atagtcgatt tctccccttt
     2641 ctgcttgaat caaaccggtt cgtctgcctt tgtcaaactg tttgaaatcg agcccatagg
     2701 ccacgatgcg cgattctttg tccccggagc gaaagatata tgcctctttt gcctgaaggt
     2761 cccattcctc tttgccatct atactgaaag acttgcggga aaaattcttg agccgaatgc
     2821 ttgcaccttt ctccagtgag tcatcctgaa gcggatcctt ttgacaggct gcgagaagca
     2881 gaatcagaag gagaaatcgg ttcatatcag ccggcgaaga tctgcgtctt gacgagtctg
     2941 tcgattttgt aaagaccacg cagcaattca ggcgcctgat ccaggtaata ctgattggaa
     3001 gagtcggacc acgcgctcgg aggatctgga tgcacttcca tgaaaattcc ctcgagtcct
     3061 gaagcaactg cagcgcgcat aagcacaggt gcaaatctac gatcccctcc actctccttt
     3121 ccggctccgg gaagttgcgt actgtgagtc ccatcgaata caaatggaat acccatctcg
     3181 tgaatgattt caggactgcg cggatcgaac actagattgt tatacccgaa agaaaccccg
     3241 cgttccgtca caaggtactt gtctgatccg caggatttga ttttgtccgc aatcttcgct
     3301 gcatcctgcg gtgccatgaa ctgtcccttc ttaacgttca cccactttcc actttccgcg
     3361 cacacctgca aaaggtctgt ttgcctcgaa agaaaagcag gaatctgaag cacatctaca
     3421 acctgagaga ctgccttgac ctgttcgcgc tcatgcacat ctgtgagcac tggaacaccg
     3481 aattccttgc gaatgttttc cagatcgcgg agcccttctt caattccagg cccacgtttt
     3541 gaatcatgcg atgatctgtt tgctttgtca aagctgctct tgaaaatata aagaatccca
     3601 agttcctccg aaagcttctt catggtttcg cacactcttt tgagcatgtc tctgctttcg
     3661 atgacgcagg ggcctgcaat caggaagaaa ggctctcgcc ctccaatttt cttgcctgcc
     3721 aggaattctc tttccttgat caacataact acctcgccag cttggaggcg gctgccacaa
     3781 aacctgcaaa cagagggtgg cacgcgattg gtttagattg aaattcagga tgaaactgca
     3841 cacccacaaa ccacggatgc gatttcaatt cgatgatttc catcagctgg ccgtccggac
     3901 tggttccgga aaaagttagg ccatgctttt ccattgcctc tttgtatctt aaagtaaact
     3961 caaagcgatg acgatgacgc tcccgaattc gagtcgtctt atactctctg gcggcgagag
     4021 tcccctcctt aatcacgcag ggataggcac ccagtcgcat ggttccgccc ttttgttcaa
     4081 ttccctgctg ttcttccaga agcgaaatca caggatgatg cgtctttgga tcgaattcag
     4141 tggaacttgc accgtgtagc cctagaacat ttcgcgcaaa ctcaatggta gcgcattgca
     4201 tgcccaggca gattccaaag aatggaatct tctttgtgcg cgcgtagtga atggacatga
     4261 gcttcccttc aataccgcgg cttccaaatc cgcccggcac cagaattccg tgcactccct
     4321 tgagtgccgc cgtagatctg gttctttcca gatcctctgg atcaatctta acgaactcga
     4381 cggtgacctg atttgcaatt cctgcatgca tcaaagactc gtatacagat ctataagcat
     4441 cctgcagcgc gatgtatttg ccgcaaactg caatgcgcac agttttgcga ggatgattga
     4501 ttacgtgtag aatctgcgcc catttcttga agttgagctt gcctggcttc attccaaaat
     4561 ggcgaagtac tcccagatcc aggccaccct gcttgtacat ttcagggatt tcgtatatac
     4621 tcgtggcaat gtcgcaggca gaaacgatcg cgccctccgg cacgttacag aacatagcaa
     4681 gcttttcttt tagat
//
LOCUS       DAOQJL010000294         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10889_length_4674_cov_4.326911, whole genome shotgun sequence.
ACCESSION   DAOQJL010000294 DAOQJL010000000
VERSION     DAOQJL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10889_length_4674_cov_4.326911"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLG78_12515"
     CDS             complement(<1..444)
                     /locus_tag="PLG78_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23988.1"
                     /translation="MRYETLRGPSISRLMSEMRQKHGSAAVLIKQREVKEGGLLGTGL
                     LSRKIYEIHYMVPEKSVEQTASKKVRPAEFTKISRQELARKLKPVFEDESLPETAAQI
                     TPAIPTPQAISEAKPEIKLERESTPQYFLKIRAQLLGAKLSATYAD"
     gene            571..1155
                     /locus_tag="PLG78_12520"
     CDS             571..1155
                     /locus_tag="PLG78_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23989.1"
                     /translation="MRFTLCLAVLFFAVSCGKHRPDPDKPPLHLFVWGKNRGDVKGLL
                     EKEGYLVSERTEFIRATVPESEGRDIMPDESPYQLTFYFQNDRLNIVQIQIRNSPDRT
                     TIYEQNLKTAYKLAQPVLQKSLPPRTTEAGNQIKETHSLYDTGEVYIKLYKSQVEIAE
                     SRMEEVPDETDIMIYSRKENEGITAEGLLSPEEN"
     gene            1214..2269
                     /locus_tag="PLG78_12525"
     CDS             1214..2269
                     /locus_tag="PLG78_12525"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNE23990.1"
                     /translation="MDLAKVRELLGGQAESLLGHKAKVAKETIHLPGPDFVDRIWTQS
                     DRSPQVLRSLQQIFSHGRLGGSGYVSILPVDQGVEHSGGASFAPNPIYFDPENIVKLA
                     IEGGCNAVCSTFGVLGSVARKYAHRIPFLVKINHNEFLSYPNKYDQVMFGTIKEAWNM
                     GAVAIGATIYFGSDESRRQIVEVSEAFAYAHELGMATVLWCYLRNNAFKVDGVDYHTS
                     ADLSSQGNHLGVTIQADIIKQKLPTNNGGYNATKHGKTHPKVYSDLSTDHPIDLTRYQ
                     VLNCYSGRAGLINSGGESKGSSDLAEAVGTAVINKRAGGMGLISGRKAFQRPFEEGAQ
                     LLQSIQDIYLNKDITVA"
     gene            2270..2899
                     /locus_tag="PLG78_12530"
     CDS             2270..2899
                     /locus_tag="PLG78_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE23991.1"
                     /translation="MDLLNSLLGVVQNFSGPLPYLFVFFILLLCGFGLPLPEDVVLFT
                     AGVLAYKGLADIHIMVAVAFAGVMIGDGTIFFLGHKLGLRLTQLPFFQRVLSPVRLAA
                     IRERFHEHGNKIVFAARFMPGLRTPVFFTAGTLHLPFRVFLFYDGMAALISVPTITYV
                     VFLFGHELEAVINTVRSVEHGMVLLIALVIAFFVGKFLWGRRKSANAPE"
     gene            2953..3753
                     /locus_tag="PLG78_12535"
     CDS             2953..3753
                     /locus_tag="PLG78_12535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE23992.1"
                     /translation="MLFFQPVGKHRFSTPLAVYALIAISVLAYAFTALDSENIIKSFG
                     LVPARFSFIDSVISTFLHSGIFHLIGNMIFLYMFGPGVEDVVGPFMFVLLYLILAVIS
                     DSCYAWMNLQSTLPVVGASGAVSGILGVFLVFFPNVKVNLFLAADSEASASGPFLMNA
                     FWAIGFWFAGQLLWAILALALPTHVAYAAHVGGLIGGVILGLLLKAAGFLDSFHARVR
                     RTRGSSKPTVYCPACALPIAPTEFGRYRCPNCASEFTFDVKRGYRLLQ"
     gene            complement(3783..4352)
                     /locus_tag="PLG78_12540"
     CDS             complement(3783..4352)
                     /locus_tag="PLG78_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE23993.1"
                     /translation="MRDVLQESVLVLNTSMIAIDICSARTAILEILRNIAIPIKESED
                     LVRSEKLTLHVPRIIAQRSYHKIPRYDQKTSKINIIYRDDQTCCYCGVRFPIPELTVD
                     HVIPRSRWKKITGETPPYDFNSWWNLVTACRRCNSVKANRLLQEIGWKLQKKPDKPKN
                     TGLVIARQRAESLGWMEYCRVNVRLVESV"
     gene            complement(4396..>4674)
                     /locus_tag="PLG78_12545"
     CDS             complement(4396..>4674)
                     /locus_tag="PLG78_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNE23994.1"
                     /translation="GKRLPFNQLPAKGRGGKGMTYIKITDKNGPAMAVATVKEDDDII
                     VVARSGMVIRLSAKEIPALGRPTVGVRVVDISEGDRVRDVAVLSDDAP"
BASE COUNT         1018 a         1158 c         1173 g         1325 t
ORIGIN      
        1 atcggcgtaa gtggcactca gcttcgcacc aagtaattgt gcgcggatct ttagaaaata
       61 ctgtggagtg ctttctctct ccagtttgat ttctggtttt gcttcggaga ttgcctgcgg
      121 cgttggaatg gcgggagtta tctgcgctgc agtttcgggc agagactcat cttcgaaaac
      181 aggcttcaac tttcgcgcaa gctcctgtct gctgatcttc gtgaattcgg ccggtctcac
      241 tttcttgctt gcggtttgtt caacggattt ctccggaacc atgtagtgga tttcgtagat
      301 ctttcgggaa agcaaccccg tgccaagtag cccgccttct tttacttctc tttgtttgat
      361 caatacggcg gctgagccat gcttttgtct catttcagac atgagtctgg aaatgctggg
      421 gccgcgcagt gtttcgtatc tcatgcgatt tttctgtatt atgtcacatt tgccgtgctg
      481 gcaattgttt ttgtagtctt gctggatttt tgcgcctttt ttcagtcttt tttgattgta
      541 taccgtcgag tggaaaccaa aggttgcgac gtgcgtttca ccctgtgcct tgcagttcta
      601 ttctttgctg tttcttgcgg gaagcacaga cccgatccag acaaaccacc ccttcatttg
      661 tttgtatggg ggaagaacag aggagatgtg aaaggccttc tcgagaagga aggttatctg
      721 gtttcagaga gaaccgagtt cattcgcgcg accgtaccgg aatcagaggg tcgcgacatc
      781 atgccagatg aaagtccgta ccagcttacg ttctacttcc agaatgacag gctcaatatt
      841 gtccagatcc agattcgcaa cagtccggat cgcacaacga tctatgagca aaacctgaag
      901 acagcctaca aattggctca gcctgtcttg cagaaatcgc ttcccccgcg cacaacagaa
      961 gcaggcaatc agattaaaga gactcattct ctttatgaca ctggcgaagt atacatcaag
     1021 ctctacaaat cgcaggtcga aattgcagag tctcgcatgg aagaagtgcc tgacgaaaca
     1081 gacatcatga tctactccag aaaggaaaac gaaggtatca ccgcggaagg ccttttgagt
     1141 cctgaggaaa actgagtttc gctggactac ctttgtccgg gtgtagaatg atgagaaaaa
     1201 aggagttcat gcaatggatc tcgcaaaagt tagagaactc ctgggtgggc aggcagagtc
     1261 cctcctgggt cataaagcaa aagtagcaaa agaaaccatt cacctgcccg ggccggattt
     1321 cgtagacaga atctggactc agtcagacag atcgccgcag gttttgcgtt ctctccagca
     1381 gatattctca cacggccgcc tcggtggtag tggatacgtt agcattctgc cggtggatca
     1441 gggagtggag cattccggtg gtgcgtcttt cgcgcccaat ccaatctatt tcgatccaga
     1501 aaacattgta aagctggcaa tcgaaggtgg ctgcaatgct gtatgcagta cctttggcgt
     1561 gctcggctcg gttgcgcgca agtatgctca cagaattcct tttctagtga aaatcaatca
     1621 taacgaattt ctaagctacc cgaacaagta cgatcaagtg atgttcggca caatcaagga
     1681 agcctggaac atgggtgctg ttgccattgg agcaaccatt tattttggtt cggatgaaag
     1741 ccgtcgccag atcgttgagg tttctgaagc cttcgcatac gcacatgaac ttggaatggc
     1801 tacagtgctc tggtgctacc tgcgaaacaa cgcgttcaag gtggatggtg tggattacca
     1861 tacttccgcg gatttaagct ctcaaggaaa tcacctgggc gtaacgatcc aggcagacat
     1921 tatcaagcag aagcttccta caaacaacgg cggatacaac gcaaccaaac atggcaagac
     1981 tcatccgaag gtgtattcag atctttccac ggatcatccg atcgatctga ctcgttatca
     2041 ggttctgaat tgctattctg gccgcgctgg cttgatcaat tctggtgggg aatctaaggg
     2101 ttcttctgac ctcgcagaag cggttggcac tgcagtgatc aacaagcgcg caggcggcat
     2161 gggattgatc tctggtcgca aagcgttcca gcgtccattc gaggaaggtg ctcagctgct
     2221 ccagtctatt caggatattt acctgaataa agatatcacg gtagcctgat tggatttact
     2281 caattcgttg ctcggggtgg tccagaattt ctctgggccg ctcccgtacc tgtttgtatt
     2341 cttcattctc ctcctctgtg gatttggtct tcctcttccg gaagatgtgg ttctctttac
     2401 tgcaggtgtg cttgcctaca agggactcgc tgacattcac ataatggtcg ccgtcgcctt
     2461 tgcgggcgta atgataggag acgggaccat cttctttctg gggcacaagt taggcctgcg
     2521 attgactcag cttccgttct ttcaacgggt gctttctccc gtgcgattgg ccgcaattcg
     2581 agagaggttt catgagcacg gaaacaagat cgtctttgcc gcgcgcttta tgcctggact
     2641 ccgcactccc gttttcttca cggcagggac tctgcatctt ccgtttagag tatttctttt
     2701 ttacgatgga atggcagcgc tcatcagcgt gcccactatc acttacgtag tcttcttgtt
     2761 tggacacgaa ctcgaggcag tgatcaacac ggttcgtagc gttgagcatg gaatggtctt
     2821 gttgattgcc cttgtgattg ctttcttcgt aggaaagttt ttgtggggca ggcgcaagtc
     2881 tgcaaatgct ccggaatgat gggatcaagc gcggcgccta acagtggtta ggccagcgga
     2941 gccaaagaat caatgctgtt ttttcagccg gtagggaagc acagattctc cacgccgttg
     3001 gctgtatatg cgctgatcgc catatccgtg ctggcttacg ctttcactgc cctggacagc
     3061 gaaaatatca tcaaatcgtt tggtctcgtc cctgcccgat tctctttcat cgattcggtg
     3121 atttcaacat tcttgcattc cggcattttt cacctcattg gaaacatgat cttcttgtat
     3181 atgtttggac caggcgttga agacgtggtc ggccctttca tgtttgttct cctttacctg
     3241 attcttgcag tgatttcgga ttcttgctac gcatggatga acctgcaatc aactcttcca
     3301 gtcgtaggtg cttccggagc tgtctcagga atcctcgggg ttttcttagt attctttccg
     3361 aacgtgaagg tgaacttgtt cttggccgca gattctgagg cctccgcatc aggtcctttt
     3421 ctgatgaatg cgttttgggc cattggattc tggttcgcag gtcagttact ctgggcaatt
     3481 ctcgcacttg ctttgccaac gcacgttgcc tatgcggcgc atgtcggggg tctcataggt
     3541 ggagttattc taggtctttt gctcaaggcc gcaggttttc tagacagctt tcacgccaga
     3601 gttcggcgga cgcgtggatc atcgaagccc acagtttact gcccggcgtg tgcgttgccc
     3661 attgcgccca ctgaatttgg gcgctatcgg tgtcccaatt gcgcgagcga gtttactttt
     3721 gatgtgaagc gcggctatcg ccttttacaa tagtaaaggc ctggtctcgc gcataaccat
     3781 cgttagacgc tttccaccag ccggacattc actctgcagt attccatcca gcccagagat
     3841 tctgctcttt gtctggcaat gaccagtcca gtattctttg gtttatccgg cttcttttgc
     3901 agcttccagc caatttcctg caagagtcga tttgctttta cggaattgca tctcctgcac
     3961 gcagttacga gattccacca ggaattgaaa tcatagggtg gtgtttctcc ggtaatcttt
     4021 ttccatctgg atcgtggaat cacatgatct acagtcagtt caggaattgg aaatcgcacg
     4081 ccgcagtaac agcatgtctg atcgtcccta taaatgatgt tgatcttgga agttttctga
     4141 tcgtacctgg ggatcttgtg ataacttctc tgtgcgatga ttcgcggcac atgaagagtg
     4201 agcttttcag agcgcaccag gtcctctgat tctttgattg ggatggcgat gttgcgcagt
     4261 atctccagaa tcgcggttct ggcgctgcaa atgtcaatgg cgatcatgct cgtattgagc
     4321 acaaggaccg attcctgtaa gacatccctc acgcgccgaa acgtaggtgc gttccagcgc
     4381 gtgtaaagaa aaaatctaag gcgcgtcgtc gctcagtaca gcaacgtctc gcactcgatc
     4441 tccctcagaa atgtccacaa ctcgaacgcc cactgtaggc cttccgagtg ccgggatttc
     4501 tttggcgctg agtcgaatca ccatgccgct tcttgcaacg acgatgatat catcgtcttc
     4561 cttcacagtc gccacagcca tggccggccc gtttttgtcc gtgattttga tgtaggtcat
     4621 acccttccca ccgcgtcctt tcgccggcag ttgattgaac ggcagtctct ttcc
//
LOCUS       DAOQJL010000295         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10913_length_4667_cov_4.470729, whole genome shotgun sequence.
ACCESSION   DAOQJL010000295 DAOQJL010000000
VERSION     DAOQJL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10913_length_4667_cov_4.470729"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLG78_12550"
     CDS             <1..1354
                     /locus_tag="PLG78_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE23995.1"
                     /translation="GPVWVRFHVRNRTAPTHWLLISEYPWIDHVDLFEHRDGNLRLVE
                     ESGDSVSVTRASVPAKQPTFALELPSNQERVYYVRFVGNAEIYIGLTLEDNLTFGTRS
                     FSVRDLFVAFVGMSLLVFVANSFVWFRLRAPLSLYYCLFNVSSLAYGCLLDGSFLRMA
                     LKTTRFSDDQLHFGIGLFAAGSILLLWTRLIPMSDLFPRLNKFSRWYAIANCAAGLLT
                     VIGVFPHLVVDVLARILMMYSNLLFYPTAIIAYRRGFRPALYPIFGMPVFSVFMFCYL
                     MSGVVFSGNLIWKNSVWYAYLVEYSVFFASMVSRIRFETSGQMETFRESLARKSRLTG
                     LDIEQLRLRLEEQLSQGVYEDETLSIAKLALLLKIRPDQLSELINVLYGMGFRRLINS
                     RRVMKAQELLETQLDRTVLAIALDTGFGTKAAFNREFKLQTGLTPTEYRRRNSTLRRP
                     "
     gene            complement(1383..1931)
                     /locus_tag="PLG78_12555"
     CDS             complement(1383..1931)
                     /locus_tag="PLG78_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23996.1"
                     /translation="MLLQNAPVLFLAFASCKQDKIPAIEKIPGKPIREQTFTFQDSLL
                     GPVTFVPVKDESAPPHEQLKIYLKLGDGSHTKLPDADINFIFHDMDAVSFVDVDENGL
                     TDIIIISQYISGIGRDAARPFRMAIIYLRRTDGFEFDFEDSWRVSELAKGNLNIARVR
                     TLFREVTRHAAKDGGTGKVRRP"
     gene            complement(2001..2561)
                     /locus_tag="PLG78_12560"
     CDS             complement(2001..2561)
                     /locus_tag="PLG78_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23997.1"
                     /translation="MEHFDKRTPIPDSARRAATVLEALLIRALAIWTMLAQLCMGLNC
                     IGLGAGFFESTERFHSGPDSNPITSSELRDREGAPDAIVKTSSETEEWYYYYYKPLGF
                     DELRTPIGYRWWGPYLHAGCIGIPLVVPIGRDYRKYVIRKQQTEASYSTDTNLCFAGC
                     MCTFVPHNDKCECSFGCDSNFANMLR"
     gene            complement(2715..3017)
                     /locus_tag="PLG78_12565"
     CDS             complement(2715..3017)
                     /locus_tag="PLG78_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23998.1"
                     /translation="MREYVPSFQRRATIRFFLKGDYTAEGAESTNVFLAADGKFSLFL
                     GFLLLAFGLFICAVGNSPVILILIPVALLYWPVTAVRFRNSIQRQVQAVRAALEAR"
     gene            complement(3195..3440)
                     /locus_tag="PLG78_12570"
     CDS             complement(3195..3440)
                     /locus_tag="PLG78_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE23999.1"
                     /translation="MKKQQIPGQSPHQIPPELIEAARNNPNSFVYKIDGSYPIRDPLG
                     DAVPASAIVGAWKVDGNGNITGDFVPNPNYRPWKPDA"
     gene            complement(3437..3904)
                     /locus_tag="PLG78_12575"
     CDS             complement(3437..3904)
                     /locus_tag="PLG78_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24000.1"
                     /translation="MKLSIAVILSMLSFACKDTRPIRQIELSKFKCDKSAKELSDQPV
                     AISGYLVPPVVIFCKSGKCSVNLQLTEEQLEPSVTIDLVPGSGPNTMDMPPENFRKTD
                     FAFRDAAGKGHRSNEQVVVTGIFDFTDAYETIPNGCRIRQVREIRPEGQFLAK"
BASE COUNT         1102 a         1154 c         1148 g         1263 t
ORIGIN      
        1 agggccagta tgggtacgtt tccatgtccg aaatcgtaca gctcccactc actggctcct
       61 gatttcggaa tatccgtgga ttgatcacgt cgatttgttc gagcaccgtg acggtaattt
      121 gcgactcgtg gaagagagcg gagattcagt atcggttacc cgtgcaagtg ttcccgccaa
      181 acagcccacc tttgcacttg agctgccatc aaatcaggaa agagtgtatt atgttcggtt
      241 cgttggcaat gctgagatct atattggcct gacactcgaa gacaatttga cctttgggac
      301 gcgatccttt tctgttcgcg atctatttgt agccttcgtt gggatgagtc tgcttgtctt
      361 tgttgcaaat agttttgtct ggtttcggct gagagctcct ctttctctct actactgtct
      421 cttcaatgtt tcgtcactcg cctatgggtg cctgctcgac ggttcgttcc tcagaatggc
      481 gttgaagacg actagattct ctgatgatca gctccatttc ggaatcggac tcttcgcggc
      541 aggaagcatt ctgcttttgt ggacaaggct catcccgatg tctgatctct ttccgcgcct
      601 gaacaagttc agtcgttggt atgcaattgc caattgcgcc gcaggtttgc tcaccgtcat
      661 cggagtcttt ccgcacctgg tagttgatgt tctggcgcgg attttgatga tgtattcgaa
      721 tcttctattc tatccgacgg caatcatcgc atacaggcgt gggtttcgac ccgccctgta
      781 tcctatcttc ggcatgccgg ttttttcggt gttcatgttt tgttatctga tgtccggcgt
      841 tgttttttct ggcaacctca tttggaagaa ctccgtatgg tacgcatacc tcgttgagta
      901 ttccgtattc ttcgcttcca tggtttccag aatccgattt gaaacgtcgg gtcaaatgga
      961 gacctttcgc gagtcactcg cacggaaatc aagactcacg ggactggaca ttgagcaact
     1021 gcgattgcga ttggaagaac aactgagcca gggagtttat gaggacgaga ctttgagcat
     1081 tgcgaaactc gcattgcttc tgaaaattcg tcctgatcaa ttgtctgagt tgatcaatgt
     1141 tctgtacggc atgggcttcc gccgcctgat caattctaga cgagtaatga aggcccagga
     1201 actccttgag acccagctcg atcgcactgt ccttgccatt gcgctggata ctggattcgg
     1261 aacgaaggcc gctttcaatc gcgaattcaa attgcagacc gggctcacgc caacagagta
     1321 ccgtaggcga aacagtactc tccggcggcc ctgacaccat gttgggaatt tcggacgcac
     1381 tttcaaggcc gcctaacttt cccagtccct ccatcttttg cggcatgcct ggtcacctct
     1441 ctgaaaagag ttcgtactcg ggcgatattt aagttccctt tcgcaagctc tgaaaccctc
     1501 cagctatctt cgaaatcaaa ctcgaagcca tccgtgcgtc taagatatat gatcgccatt
     1561 cgaaagggtc gggctgcatc gcgtccaata cctgatatgt actgagatat gatgataatg
     1621 tctgtcagac cattttcgtc cacgtccaca aaggatacgg catccatatc gtggaagatg
     1681 aagttgatat cagcgtcagg gagttttgta tgcgagccgt cgccgagttt caagtagatc
     1741 ttgagttgtt cgtgcggagg agccgattca tcttttacag gaacaaaagt gaccggtccc
     1801 agcaatgaat cctgaaaagt gaaagtctgt tcacggatcg gtttgccagg gattttttca
     1861 atggcaggaa tcttgtcttg tttgcaggat gcaaacgcca ggaagaggac tggagcattc
     1921 tgaagaagca tttcgattag tctcggtaag attcttctca tgtcagtatt cccctgattc
     1981 ttcgtgactc gcgctctttc ctaccgtagc atgttcgcga aatttgagtc acaaccgaaa
     2041 gagcactcac atttgtcatt gtgcgggacg aacgtgcaca tgcatcccgc aaaacaaaga
     2101 ttggtatccg tggaatagga agcttctgtc tgttgttttc ggattacata cttgcggtag
     2161 tcacgcccaa tgggaactac aagtggaata ccgatgcagc cagcatgcag atacggcccc
     2221 caccatcggt agccaatcgg agtccttagc tcgtcaaagc cgaggggctt gtagtaatag
     2281 tagtaccact cctcagtctc agaactggtt tttacaattg catctggtgc gccctcacgg
     2341 tccctcagtt cgctggacgt aattggattt gagtctggcc cagaatgaaa gcgctcggta
     2401 gattcgaaga aaccggcacc gagcccgatg caattcaggc ccatgcacaa ttgcgcgagc
     2461 atggtccata tggcgagtgc gcgaatcaac aatgcctcca gtacagttgc agcgcgacga
     2521 gcagaatcag gtatgggagt acgcttatca aaatgttcca tgcaaaccgc aaataggcac
     2581 gaatcgtaac gtagaacaag tagaaagtgc ctacaagcat tgaggcggca aagcaaattg
     2641 cgcctatgcg accaagtagc ccggccacgt tggctgcaaa atcattcatg atatcaacgt
     2701 ttcgtcgttg gatttcatct cgcttccagt gcggcgcgga ctgcttgaac ttgtcgttgg
     2761 atgctattcc tgaaccgcac agcagttacg ggccagtaga gcagcgcaac cgggattaga
     2821 atcaagataa ccggggagtt tcctacggcg cagataaaca aaccaaatgc cagcaacaag
     2881 aagccaagaa agagggagaa cttcccgtct gcagctaaga acacgttcgt ggattcagcg
     2941 ccctccgccg tgtagtctcc ttttagaaag aagcgaatgg ttgcgcgcct ctggaaggaa
     3001 gggacgtatt ctcgcatcca gaaactgcct ccactgcact caccttcaaa cccgcgtcca
     3061 gaaacccatc ccttttctgg agtaagtaat ttgtgaattc ggatctctac ctgatctata
     3121 cggaggggta cccggaagga aaaccctatg ctcattttcc aaaaatgttg ctgtaattct
     3181 ttcatgtgcc cgggctacgc atctggcttc catggtctgt agttgggatt gggaacgaaa
     3241 tcgccggtga tgtttccatt accatctact ttccatgccc ccacaatggc agatgctggt
     3301 acagcgtcgc ctaacggatc acggatagga taggaaccat caattttgta aacgaagctg
     3361 tttggattgt tgcgtgcagc ttcgatcagc tccggcggta tttgatgagg gctttggccc
     3421 ggaatctgtt gttttttcat ttcgcaagaa attgtccttc agggcgtatt tcgcgtactt
     3481 gccttatcct gcacccgttc gggatcgtct cgtaggcgtc cgtgaaatca aaaattcccg
     3541 ttacaactac ttgttcgttc gatctatggc ctttccctgc agcgtccctg aacgcgaagt
     3601 ctgtttttct gaaattctca ggaggcatat ccattgtgtt aggtccgctt ccgggcacaa
     3661 ggtctatagt aacagagggt tcgagctgct cctctgtcag ttgcaggttc acagagcatt
     3721 tgccagactt gcaaaaaata accacaggcg gtacgagata gccagaaatt gctactggtt
     3781 gatctgagag ttcttttgca gacttatcac acttgaattt ggagagctcg atttgtctga
     3841 tgggcctggt gtctttacac gcaaatgaca gcattgaaag aatcaccgcg atcgaaagtt
     3901 tcatagccaa gcaggctgag tattctttgg tcagtcaaat ggaattggcg gatggatccg
     3961 ccatttgcgc atgagtcgcg attctctgga aagtgcaatg gttccttcgg ttcatttcag
     4021 ccaagcccgt ttttgcagga ccagccaatc gtgcagtaaa ggatcaacaa gtagttgaaa
     4081 ggcccaaaat agagcagctg aaacggggca gttacaatat caactggaac cgttaaagga
     4141 tagaggaaat atgcatggcc gattctagtt tcacgctcgg gttgaaggat tcctaaggat
     4201 atcgtcagct caggagaccc gttcctcccg cgccatttca gttccgtgat ttccgctctg
     4261 gaaatctcgt tgctccgact cctcgattga gacaatcaat gtcggctttc tggtggtctc
     4321 atcgtttttg gccccaggcg gtttttcgtg gtggctgtta agcaattgca tacaaacaag
     4381 aatgtcgcaa tcgcccacaa acctggagcg gacccgttta tcagattcca ggttttgttt
     4441 ttgaaatgct attcctcaca acctgccatg atcctctctt ttttcgtctg ttctccgctc
     4501 gactatacat atttcgaaac aagctctata aggattcttg gcgcaatccc tgcgaaggca
     4561 ccggcgagga tcgcggcggc aatccaccag ttcatgatga actcgacact tgcagggttc
     4621 agttgtttac cggatgacaa tcggaccggg cctgccacat aggcaac
//
LOCUS       DAOQJL010000296         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10936_length_4662_cov_4.800304, whole genome shotgun sequence.
ACCESSION   DAOQJL010000296 DAOQJL010000000
VERSION     DAOQJL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10936_length_4662_cov_4.800304"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLG78_12580"
     CDS             complement(<1..726)
                     /locus_tag="PLG78_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24001.1"
                     /translation="MEFLVFALLNLVTGLVLYVLFSLRMQAWQNKIRAAHPLQKDVEE
                     NIRQGLQAMNAYLEEMQEIRANFYQLVRHAEDLKKEETRKPRKKKREEPELKSLLPEE
                     EEQEDHVSRILDKIGPDTVDLTRPAAPQPRSQPPVQQPEPSAGILQGIGRFAASIFGV
                     KTDPFAASIGKATPAEEALSIDVPQPTIRPPVSLNVEPARESVGLPSASDSTAASAPI
                     TQDPNAWIRSLLAGGMKAADVSRV"
     gene            complement(729..1058)
                     /locus_tag="PLG78_12585"
     CDS             complement(729..1058)
                     /locus_tag="PLG78_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24002.1"
                     /translation="MRLLDLQVSYQALPEQARLQGMEHAAGAYRQVAAMALARQENLE
                     RPEKVIQAASTTSASMAAIDPREKRMQSQQQPSKSSREVETFIYNERGIKRKPPTPVG
                     SKLDLFI"
     gene            complement(1055..3031)
                     /locus_tag="PLG78_12590"
     CDS             complement(1055..3031)
                     /locus_tag="PLG78_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNE24003.1"
                     /translation="MDRLKQALMESDSQELNLVKDRVEVIGASIAGCLARAASHLGQE
                     VSRLDYEILERGKSTLLTKIPYRLLVSVLPPEERFADLEEFSIKLGVGDRMLSEELDQ
                     FVAPKHMDGRCLVRVYRSGVYVTVFPPLGQGMPVDLETVLNRVRSTGVQNFVQSTVEK
                     AVRDASGEPVRIGDTVPRPEADSSCKVDISDDEMTATVRITAPKMGGRHLEVNDVVNA
                     LKAHGVVIGFKEDLIRSALIEDRYMQDIVAAEGVPPLHGPDAKIEYKVNIKKNVQLEQ
                     DDRGKVDFKNLNLVENVVAGQILGVKIPAKKGVPGRTLTNRIVLARDGKDIELRQGKG
                     TILSDDKTKLIAEINGQVVYSNLKLSVEPVYRVVGDVGPKTGNIMFLGSVIVGGSVLD
                     NFEVKAAGNVDVHGSVGKARIEAEGDIIVKQGILGREGAFIESTSGSLFAKFIQSARV
                     NVAEDVVVQEGILHSEVDAGGKVVCNGRRAQIVGGAIRAKKEVRARMIGSPSYTGTEI
                     TVGTDPRVLKQFDELRALSKDTEDKLSRAKKSLATLNARKKADVDSFSPAQEEQLQKA
                     EDASQKLTARTKEIAEEMGKLEEYMNQLSAQGKVHIEKEVFPNVTVTIRNVSQTFSDT
                     YRAVTLTFENGVVKFNKLEKAEEKAAPTRGGRRK"
     gene            complement(3031..3786)
                     /locus_tag="PLG78_12595"
     CDS             complement(3031..3786)
                     /locus_tag="PLG78_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24004.1"
                     /translation="MDFERRIQESVARKKILIKSYLLREEGEEKLERIIHAVLRSCGR
                     EDLMGPIYACMRELVQNAAKANLKRVVFEEQGLNPFDEDQYRFGLEAFRKSLASKRIQ
                     HYRPILHARDVPFYVKIKWDGRVLLLNVENYFAMLTVEEKRIREKFIHSHNMDNLYEF
                     FMTYGDSTEGAGMGIAMVQILMKQAGFDAHNFTIFSDPIRNRTSARLVVPLVKDYSEP
                     RVRFAEEMKRRGVEASVLRKEIQEGKLEFPVFY"
     gene            complement(3786..>4662)
                     /gene="thrC"
                     /locus_tag="PLG78_12600"
     CDS             complement(3786..>4662)
                     /gene="thrC"
                     /locus_tag="PLG78_12600"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE24005.1"
                     /translation="STGDTSAALAAYAASAGIPTVVFLPAGKVSNAQLVQPVSSGSLV
                     LSLETDFDGCMQIVKEVTANKTLYLANSMNPLRIEGQKTISFEITQQMGWEVPDWVVI
                     PGGNLGNVSALAAGFEMLLETGIISKRPRILLAQSARANPMYLSYKNNYRYSPVQASQ
                     TLASAIQIGNPVSYPRARRALEKYNGIVEQATEQELADAAARADLHGLFNDPHTGVAL
                     AALIKCRERGDIEADARVVVISTAHGLKFQEFKAGYHEGTLADVSGKYRNSVKKLRAN
                     VSDVQSALEEFLRVN"
BASE COUNT         1041 a         1292 c         1101 g         1228 t
ORIGIN      
        1 tacacggctt acgtctgcag ctttcattcc accggcaaga agacttctga tccacgcatt
       61 cgggtcctga gtgatcggag cggaggcggc ggtagaatcg gacgcagaag gaaggccaac
      121 gctctctcgc gcaggttcca cgttaagcga tacaggcggg cgaattgtag gttggggtac
      181 atcgatagac agcgcttcct ctgcgggagt tgccttcccg atggatgcag caaatggatc
      241 cgtttttaca ccgaagatgg aggccgcaaa tcttccaatg ccctgaagaa ttccagcaga
      301 tggttctggc tgctgaacgg gaggctgact acgaggttgc ggagcggcag gacgcgtcaa
      361 atcgacagta tctggtccaa tcttatctag aattctgctt acatgatctt cttgctcttc
      421 ttcttccgga agaagtgact taagttccgg ctcttcgcgc tttttctttc ttggcttgcg
      481 agtctcttct tttttgagat cttccgcatg gcgaacgagc tgatagaaat tcgcacgaat
      541 ctcctgcatc tcttcgagat aagcgttcat ggcctgaaga ccctgacgaa tattttcttc
      601 tacgtctttc tgcaaaggat gggcggcgcg aatcttattt tgccacgctt gcatgcgaag
      661 ggaaaagagc acataaagaa caaggcccgt aaccagattg agcaatgcga agaccaggaa
      721 ttccattctt aaataaacaa atcgagtttt gaaccgactg gagtcggagg tttacgtttg
      781 attccgcgtt cgttataaat gaacgtttct acttcccgcg aagattttga tggctgctgt
      841 tgcgattgca tgcgcttttc gcgcggatca atggcagcca tagaagcact cgtcgtcgac
      901 gctgcctgga ttaccttttc aggacgctcc agattctcct gacgcgcaag cgccatggca
      961 gccacctggc gatacgctcc ggccgcatgc tccatcccct gcaggcgggc ctgttccgga
     1021 agtgcctgat acgatacctg taagtccagg agcctcattt tcttctgcca ccgcgcgtag
     1081 gcgctgcttt ctcttcagct ttttcaagtt tgttgaattt tacaaccccg ttttcgaacg
     1141 tcagagtcac ggcccgatat gtatccgaaa aggtctggga aacattgcga atggtgaccg
     1201 taacattggg gaagacttcc ttctctatat gaacttttcc ctgtgcagac agctggttca
     1261 tgtattcttc gagcttgccc atttcttctg cgatttcttt ggttctggca gtcagcttct
     1321 ggcttgcgtc ttccgccttc tgcagttgct cttcctgcgc cggtgaaaag ctgtccacgt
     1381 ctgctttctt gcgagcgttc aaagtggcca gagacttctt cgcacggctc agcttgtctt
     1441 ctgtgtcttt ggaaagcgcg cgcagctcat caaactgctt gagcacgcga ggatctgtgc
     1501 caactgtgat ttctgtgcct gtgtaagacg gggagccaat catccttgcg cgaacttctt
     1561 tcttcgcacg gattgctccg cctacaattt gcgcacggcg gccattgcag actacctttc
     1621 cgcctgcatc tacctcgctg tgtagaattc cttcctgaac cacaacgtct tcagccacat
     1681 tgacgcgcgc actctggatg aattttgcaa agagagaacc actggtcgat tcaatgaatg
     1741 caccttcgcg tccaagaatc ccctgcttca caatgatatc gccttctgct tcgatgcgag
     1801 ctttgcccac agagccatgc acgtccacat tccccgcagc tttcacttcg aagttgtcga
     1861 gcacacttcc gccaacaatg acggatccaa ggaacatgat atttccagtc ttcggaccaa
     1921 catcgccaac gactctgtaa acaggctcaa cagaaagctt caggttggag tacactacct
     1981 gtccattgat ctctgcaatc agctttgtct tgtcgtcaga taggatggtg cctttgccct
     2041 ggcgcagttc gatgtctttt ccatcgcgcg ccagaacgat tcgattcgtg agggttcttc
     2101 ccgggacacc tttcttcgct ggaattttca cgccgagaat ctgacctgcg accacgttct
     2161 ccactagatt caggttcttg aaatccacct taccacgatc atcctgctcg agctgaacat
     2221 tcttcttaat atttactttg tattctatct tcgcatctgg tccgtgaagt ggcggcacac
     2281 cttctgccgc aacaatgtct tgcatgtaac gatcttcaat gagagcggaa cgaatcagat
     2341 cttctttgaa gccgattacc acgccgtgag ctttcagcgc attgaccaca tcgttcactt
     2401 ccagatgtcg gcctcccatc ttcggagccg ttatgcgaac tgtggcagtc atttcgtcat
     2461 cagaaatatc aaccttgcaa ctagaatccg cctctggcct gggcactgtg tctccgattc
     2521 gcacgggctc accggaagca tcgcgcactg ccttttctac agtggattgt acaaaattct
     2581 gaacaccggt agaacgtacg cggttgagaa ccgtttccag gtccaccggc attccttgac
     2641 cgagtggggg aaataccgta acgtagacac ctgacctgta cacacgaacc agacaacgac
     2701 cgtccatatg tttgggtgcg acaaactgat ccagttcttc gctgagcatt ctatctccca
     2761 cacccagctt gatcgagaac tcttctagat ctgcgaagcg ctcctctgga ggcaggacag
     2821 aaaccagaag gcgataggga atctttgtca gtagagtgga cttacctctt tccagaatct
     2881 catagtcgag gcgtgatact tcctgaccaa ggtgagatgc agcgcgagcc aggcaaccag
     2941 cgatgctcgc accgatcact tccacccggt cctttaccag gttcagttcc tggctgtcgg
     3001 actccatcaa tgcctgtttc agccgatcca tcagtaaaac acgggaaact ccagtttccc
     3061 ttcctgtatt tcttttcgga ggactgaggc ctccactcct ctccttttca tctcttccgc
     3121 gaagcgcacc cttggctcag aataatcctt caccagcgga accacgagcc tggcagaggt
     3181 tcggttgcga atcgggtccg aaaatatggt aaaattatga gcgtcaaagc ccgcctgttt
     3241 catgaggatc tgcaccattg cgattcccat cccggcgcct tccgttgaat ctccatacgt
     3301 cataaagaat tcgtacagat tgtccatgtt gtgagaatga atgaatttct ctctgattct
     3361 tttctcttcc acggtaagca tggcgaagta gttctcaacg ttgagaagaa gcacacgtcc
     3421 atcccatttg atcttcacgt agaaaggcac atcgcgtgcg tgtagaatcg ggcggtaatg
     3481 ctgaattctc ttggaggcaa ggcttttgcg gaacgcctcg agaccaaagc gatactggtc
     3541 ttcgtcgaaa ggattgaggc cctgctcttc aaaaacaacg cgcttcaggt ttgccttggc
     3601 cgcgttttga accagctcgc gcatgcaggc atagattgga cccatgaggt cttcgcgtcc
     3661 gcaagaacgc aacacagcgt ggatgatcct ctcaagcttc tcctctccct cctctcgaag
     3721 tagataagat ttgatcagaa tcttctttcg cgcgacagac tcttgaattc tacgttcaaa
     3781 gtccatcagt tcaccctcaa gaactcctcg agcgctgact ggacatccga tacgtttgca
     3841 cggagcttct tcacagaatt gcgatacttc ccggacacat cggccagtgt accttcgtgg
     3901 taccctgcct tgaattcctg aaacttcaag ccatgtgcag tgctgattac aaccacgcga
     3961 gcatccgcct ctatgtctcc acgctctcta catttgatga gcgctgctag cgccacacca
     4021 gtgtgaggat cattgaacaa tccatgcaag tctgcgcgag ccgccgcatc cgccagctcc
     4081 tgctccgttg cctgctcaac gattccattg tatttttcaa gtgcgcgacg cgcacgcggg
     4141 tagctcacgg gatttccgat ctgaatggca gaggccaatg tctgggatgc ttgcacgggc
     4201 gaataacggt agttgttctt gtaactcagg tacattggat ttgccctggc actctgtgcg
     4261 agaaggatgc gcggtctctt cgaaatgatt ccagtttcca ggagcatttc aaaaccggcg
     4321 gcaagcgcgc tgacatttcc caggtttccg cccggaatga caacccagtc tggcacttcc
     4381 catcccattt gctgcgtaat ttcaaaactg attgtctttt gtccttcgat gcgaagagga
     4441 ttcatcgagt tggccagata aagcgtcttg tttgcagtga cttcttttac aatctgcatg
     4501 catccgtcaa aatcagtttc cagactcaga accagacttc cagacgacac tggctgcaca
     4561 agttgcgcat tggatacttt acctgctgga agaaagacaa ccgttggaat tccggcggaa
     4621 gcagcatacg ccgcgagagc agcactcgtg tctcccgtag aa
//
LOCUS       DAOQJL010000297         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_10938_length_4662_cov_3.200998, whole genome shotgun sequence.
ACCESSION   DAOQJL010000297 DAOQJL010000000
VERSION     DAOQJL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_4662_cov_3.200998"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLG78_12605"
     CDS             <1..289
                     /locus_tag="PLG78_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24006.1"
                     /translation="NFWLMIFAMVSVGFFVYGPQVLVAVVAAENVDARAVGTAVGMTG
                     FFGYVGATICGVGTGYVVDHFGWDVALVVYLISALIGAGLFAIAWKPVMRR"
     gene            374..1285
                     /gene="deoC"
                     /locus_tag="PLG78_12610"
     CDS             374..1285
                     /gene="deoC"
                     /locus_tag="PLG78_12610"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNE24007.1"
                     /translation="MKSAAVVPDFRYTPVIDQVGVEERVARFSSRSIKKESKVQGLKL
                     ALSMIDLTTLEGKDTEGRVRQMCYKAAHLHDVLPGLPTVAAVCVYPTHVKTAKDALKG
                     TGIKVASVATAFPSGNATLDVKIADTKFAVESGADEIDMVIARGEFLRGNYSFVFDEI
                     AAVKEACGKARLKVILETGELSTLDNVRRASDIAIYAGADFIKTSTGKIQPAATMPVT
                     LVMLDAIKDYYYQTGTMIGMKPAGGISTAKLALQYLVMLYETLGSKWMTNQWFRFGAS
                     SLANDILMQLAKEAHGVYQSPDYFSKD"
     gene            1294..2769
                     /locus_tag="PLG78_12615"
     CDS             1294..2769
                     /locus_tag="PLG78_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE24008.1"
                     /translation="MATQKKEGPRLDFFSDWKYSPAPESKEHIRLNKKYDLFINGKWQ
                     KPTGSKYFATHNPATEEKLAEVADAGEKDVDAAVQAARSAYEKTWRKMPGRERGKYIY
                     RIARMIQERARELSVIETMDGGKSIRESRDIDVPLAASHFFYYAGWADKLDYAFPNRK
                     PTPLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAETTPLTALKLAEIIQDSGL
                     PDGVVNIITGAGKTGAAIVNHPHVNKVAFTGSTDVGRIIQKAVAGTAKRVTLELGGKA
                     ANIIFEDAAIDQAVEGIVNGIYFNQGHVCCAGSRLFVQEGVADLVIRKLKDRMETLIV
                     GDPLDKNTDVGAINSKQQLQVITNYIQIGKEGGAEFYQSSCELPKKGYFVRPTLFLNV
                     SQSHRIVQEEIFGPVLAVQTFRTIEEVIEKANNIPYGLSAGVWTDKGSKIFNLTGKLR
                     AGVIWANTYNKFDPTSPFGGYKESGFGREGGVHGLLPYLSL"
     gene            2776..3657
                     /locus_tag="PLG78_12620"
     CDS             2776..3657
                     /locus_tag="PLG78_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE24009.1"
                     /translation="MGRVEVLKTYKLYIGGQFPRTESGRYYPLLDAKGRTIANVSLSS
                     RKDFRNAVVAARAAQGSWAGKTAFNRSQILYRIAEMMEGRFEQFVSEIVLQGKSRKAA
                     EEETRTAIDRVVYYAGWCDKFQALFSTVNPVASSHFNFSVPEPTGVVAIIADPKTSLI
                     GIVSAIMPVIAGGNTCVALASESLPLCAVTLGEVLETSDLPAGIVNILTGSALELAAH
                     FASHMDVNAVVDCSGSVERKKLIQEAAALNIKRAFFYEKDWIKPDAESPYLILDLQEI
                     KTTWHPIENIGVGGAKY"
     gene            3641..>4662
                     /locus_tag="PLG78_12625"
     CDS             3641..>4662
                     /locus_tag="PLG78_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24010.1"
                     /translation="MARSTELTFNCKRFVVFRRQSASRQKGGGMQGAVRNAFAALFFS
                     LLVASCGPRHKENPLLSLLLGTTPWAVPVFSYAGPGNQGTNMENLLVATIGTATSSIQ
                     CGFQDVNLGSVMNALQFAKSRGIVVRVGIDEDNRNMPGYRRLSGFLSTQGDDHEFWLG
                     NSGSGQAYTNICVADKSRVFISTAPPTMQGFYSEPAFAMYFQDEETELPRKFQSEVDL
                     LTHGSFGSNKQVLNRRNYYLLSDMEVGVYFAPKESAFEKFTVTRVKQARTSIQIFSSE
                     FFSNELDGSSFREASDLAFETRFAAAPTKQVVVTTYAAQTADPDASGGVNSANYLASN
                     GIGYKT"
BASE COUNT         1182 a         1094 c         1276 g         1110 t
ORIGIN      
        1 taatttctgg ctgatgattt ttgctatggt ttctgtagga ttctttgtct acgggccgca
       61 ggttctggtg gcggtggtcg cggcggagaa tgtggatgcg cgcgcagtcg gcacagcagt
      121 tggaatgaca gggttctttg gctacgtggg agcaaccatt tgcggagtcg ggactggtta
      181 tgtcgtagat cacttcggtt gggatgtcgc cctggtcgtc tatttgatat cggctctcat
      241 tggtgcgggc ctcttcgcga tcgcctggaa gcccgttatg cgaagatagg cactcttcgc
      301 gcgctttttg attgactggt tttcctttcg agttcatttt ctgcacagag ggcgcacagc
      361 cttcgtacat cgaatgaaat cagcagctgt tgttccagac tttcgctaca ctcccgtgat
      421 tgaccaggtt ggagtagaag agcgcgtggc gcgtttcagt tcgcgcagta ttaagaagga
      481 gtccaaggtc caggggctca agcttgcact gagcatgata gacctcacca cactcgaagg
      541 caaggacaca gaagggcgag tgcgtcagat gtgttacaag gcagcgcacc tccatgatgt
      601 tcttcctgga ttgccaacgg ttgccgcagt gtgcgtgtat ccaactcatg tgaagactgc
      661 gaaagacgca ctgaaaggaa caggaatcaa agtagcctct gtcgccactg catttccaag
      721 cggcaatgcc actcttgatg tgaagattgc ggatactaag ttcgccgtgg aaagcggagc
      781 ggacgaaatc gatatggtaa ttgcgcgagg ggaatttttg cgaggcaact attcgttcgt
      841 gtttgatgaa atcgcggcag tgaaagaagc gtgcggcaaa gctagattga aagtgattct
      901 ggaaacagga gaactgtcca cactggataa cgtcaggcga gcaagtgaca ttgccattta
      961 cgcgggagcg gattttatca aaacttctac cggaaaaatt caacccgcag ccacgatgcc
     1021 cgtgactctt gtcatgctcg atgcaatcaa ggattactat taccagacag gaaccatgat
     1081 cgggatgaag cccgcgggag gaatttcaac cgcgaaactc gccctgcaat atctggtgat
     1141 gctctatgaa accctgggaa gcaagtggat gaccaatcaa tggtttcgct ttggcgcatc
     1201 cagtcttgca aacgacatcc tcatgcagct tgcgaaggaa gcgcacgggg tgtaccaatc
     1261 accggactat ttttcgaagg attaggagac cgcatggcaa cgcagaagaa agagggcccg
     1321 cgtctggatt ttttctcgga ctggaaatat tcgccggctc cagaatcaaa agaacacatt
     1381 cgactgaaca aaaagtatga tttgtttatc aacggcaaat ggcagaagcc caccggatca
     1441 aagtatttcg ccacgcacaa tccggccaca gaagagaagc tcgctgaagt agcggacgcg
     1501 ggggaaaagg acgtagatgc agcggtccag gcagccagat ccgcgtatga gaaaacgtgg
     1561 agaaagatgc ccggtcgcga acgcggtaaa tacatttatc gcatcgcacg aatgatccag
     1621 gaaagagcgc gcgaactctc cgtgattgaa accatggacg gcggaaagtc cattcgagaa
     1681 tcgcgcgaca ttgatgtgcc acttgcagcc agtcatttct tctactacgc cggatgggcg
     1741 gataagctgg actacgcttt tccaaatcgc aaaccgactc cgctcggagt ggccggccag
     1801 atcattccgt ggaattttcc attgctcatg gcggcgtgga aaatcgcacc agctctagca
     1861 tgcggaaata cagtcgtttt gaaaccggcg gaaacaactc ctctcactgc gctgaaattg
     1921 gctgaaatca ttcaggatag cggccttcca gacggagtgg tcaacatcat tacaggcgcc
     1981 gggaaaaccg gggccgccat tgtgaatcat cctcatgtaa acaaggtggc cttcaccggc
     2041 agtacggacg tgggaagaat catccagaaa gccgtcgccg ggactgcaaa gcgcgtaact
     2101 ctcgagttag gtggtaaagc tgccaatatc atctttgaag atgcagccat tgaccaggca
     2161 gtcgaaggca ttgtgaatgg aatctatttc aatcagggtc atgtgtgctg tgctggatcg
     2221 cgtctctttg tccaggaagg agtcgcggat cttgtcattc gcaaactgaa agatcgcatg
     2281 gaaacgctca tcgtggggga tcctctcgac aaaaatacgg atgtgggcgc catcaactcc
     2341 aagcaacagc tccaggtaat caccaactac attcaaattg gaaaggaagg cggagctgag
     2401 ttttatcaga gttcatgcga acttccaaag aaaggatact ttgttcgtcc cactctcttt
     2461 ctcaatgtat cccagtctca tcgcattgtt caggaagaga tcttcgggcc agtactggcc
     2521 gtgcagacct ttcgcacgat tgaagaagtg attgaaaagg caaataacat tccctacggg
     2581 ctctctgccg gagtatggac ggacaagggt tcaaagatct tcaatcttac tggaaagctt
     2641 cgcgcaggag tgatctgggc caacacttac aacaagtttg atcccacatc gccttttggc
     2701 ggttacaagg agagcggctt tggtcgggaa gggggcgtgc atggtctcct tccttatctg
     2761 agtctgtgag gaagtatggg tagagtagaa gtacttaaga catataaact atatattggt
     2821 ggtcagtttc cgcgcactga atcgggaaga tattacccgc ttctggatgc gaaaggtaga
     2881 acgattgcga atgtaagtct ctcttcgcga aaagattttc gcaatgccgt tgtggccgcg
     2941 cgagcagccc agggttcatg ggcaggaaag actgcattca atcgatctca aattctatat
     3001 cgcattgcag agatgatgga aggtcgtttc gaacagttcg tgtctgaaat cgtattgcag
     3061 ggaaaatcca ggaaagcggc agaagaagag actcgcactg caatcgatcg tgtagtttac
     3121 tacgcaggct ggtgcgataa gttccaggct ctctttagta cggtcaatcc ggttgcttca
     3181 tcgcacttca atttctcggt gccggagcca acgggagtcg tcgcaatcat cgcagatcca
     3241 aaaacatctt tgattggaat cgtttccgcg atcatgcctg tgatcgcagg gggaaacact
     3301 tgcgttgccc ttgcgtctga atctttgccg ttatgcgccg ttacactggg agaagtgctc
     3361 gaaacatctg atttgccagc cgggattgtg aatattttga ccggatctgc cttagagctt
     3421 gcggcgcatt tcgcttcgca catggatgtc aatgcagttg tcgattgcag tgggtcggtg
     3481 gaaaggaaga agttgattca ggaagcagcc gctttgaaca tcaagcgtgc ctttttctac
     3541 gaaaaggatt ggatcaagcc ggatgcggaa agcccgtatt tgattctgga tctccaggag
     3601 atcaagacga cctggcatcc gatcgagaat attggagtgg gtggcgcgaa gtactgaact
     3661 gacgttcaat tgtaagcgtt ttgtagtctt tcgaagacag agtgcttcga ggcagaaagg
     3721 aggggggatg caaggtgcag tgcgaaatgc tttcgcggcg ttattctttt cccttctggt
     3781 cgcgtcttgc ggtcccaggc ataaggagaa tcctcttctc agtctgcttc tgggaacgac
     3841 tccctgggcc gttccagtat tttcgtatgc tggcccagga aatcagggaa cgaacatgga
     3901 gaacttgctt gtggcaacga tcggaacggc cacatcgagc attcagtgcg ggttccagga
     3961 tgtgaatctg ggaagcgtaa tgaacgcact tcagtttgcg aaatcacgcg gcattgtggt
     4021 gcgagtggga atagatgaag acaatcgaaa catgcctgga tatcgccggc tttctggatt
     4081 tctaagtacc cagggagacg atcacgaatt ctggcttgga aattctggtt ctggccaggc
     4141 atacacgaac atctgcgttg cagataagtc gcgcgtgttc atctctacag cgccgcccac
     4201 catgcaaggc ttctatagcg agcccgcatt tgcgatgtat tttcaggatg aagagactga
     4261 gcttccgcgc aagttccagt ccgaggtgga tctactcacg catggatcct ttggctccaa
     4321 caagcaagtc ctcaatcgtc gcaactacta cctgttaagc gacatggaag tgggagtcta
     4381 tttcgctccc aaagagagtg cattcgagaa attcactgta accagagtca agcaggcacg
     4441 cacatcgatt caaattttct cgagcgagtt tttctcaaac gaactggatg gatccagctt
     4501 tcgcgaagcc agtgatctcg cattcgaaac cagattcgca gccgcaccaa ccaaacaggt
     4561 tgtagtgacc acctatgctg cgcagaccgc ggatcctgat gcatccggag gcgtaaattc
     4621 agctaactac cttgcatcca atggaatcgg gtataagacc at
//
LOCUS       DAOQJL010000298         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11040_length_4630_cov_4.473443, whole genome shotgun sequence.
ACCESSION   DAOQJL010000298 DAOQJL010000000
VERSION     DAOQJL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11040_length_4630_cov_4.473443"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLG78_12630"
     CDS             <1..960
                     /locus_tag="PLG78_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HNE24011.1"
                     /translation="QGDNAFELKNAMAKEIDQHLRALIDIANGRDAVGRPMFGGDIVE
                     RMPFEPVVSNIRGGKGIELDNMIVGVEYRGNIGQQLREVERSQYIDVNLPGNKVFWGT
                     NMTVTGSVDNSGYRATTDQVFKIDGQTIRVAAGDTIDDIIDKINNASLEVKASKIGQD
                     FASLHTTNPHQVWLEDIEGGTVLRDIGLINPDKANPPNNFAETARVSGLSVFDVLIKL
                     RNDMIAGDQLEISGRDLGNLDEGLNNILRYRAEVGARQNRMDEHEKRVVWDQTYMTEL
                     LSKSEGVDMPEAIVNLKYLEAVHQYALNIGARLMKPTLMDFMR"
     gene            971..1423
                     /gene="fliW"
                     /locus_tag="PLG78_12635"
     CDS             971..1423
                     /gene="fliW"
                     /locus_tag="PLG78_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNE24012.1"
                     /translation="MTKTGRTLETKALGKITLDPEQLLSFPEGLYGFRDFHEFALVEE
                     SAENPFKWLQSTSDPALAFIVIQPELILSGYRPEVAREDLQSIGLSGVDESLVFLIVT
                     IPENDPESMTANLQGPVLINKTTKQGRQAISMNEKHVVRLPVLSQVDA"
     gene            1423..1659
                     /gene="csrA"
                     /locus_tag="PLG78_12640"
     CDS             1423..1659
                     /gene="csrA"
                     /locus_tag="PLG78_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNE24013.1"
                     /translation="MLVLARKINESIMIGDDIEIVVVDIKGDQIKLGIRAPRSVSVHR
                     AEIFKEIQEQNKKAVESAPQSLGKLKNLLDKKKQ"
     gene            1682..2035
                     /locus_tag="PLG78_12645"
     CDS             1682..2035
                     /locus_tag="PLG78_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24014.1"
                     /translation="MRAVLFIFLSCFSATLGAEPDGSKMDAHTSINYRITNRKYVSHI
                     LLERSGGINYVTLLTTHPMQWAFHSESHALKYACGKIPDCMDLMSRLDECLNAGKNLN
                     LHLDGERIIEIQVYE"
     gene            complement(2040..2900)
                     /locus_tag="PLG78_12650"
     CDS             complement(2040..2900)
                     /locus_tag="PLG78_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24015.1"
                     /translation="MRLLNAAILAVTLHQTACITTALHTMAFPDGYDFEHAQDIHDLE
                     ASGNSFVFCAQSPRTTRQIKVTGDSSRSYAMSSETLSIFETQKVWETNITDGCKAKGP
                     TGSFETAGTLFFSTQSVSMTAMKSRPVAADVPAARVAKLETDENQTRIRATLENGAVL
                     ELRRAGPGEFGIYNGTRETPFVPIASGKAENSTQWKEQPLRQLSGAVGIYFIRPDGNM
                     ALKLFDGVRPAGSETALIYVPYVSMSREKNRYYLLPLYPFTMILDVLTIALQIAIWDE
                     EIDTWTRCLK"
     gene            2987..3505
                     /locus_tag="PLG78_12655"
     CDS             2987..3505
                     /locus_tag="PLG78_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24016.1"
                     /translation="MRNLFILAVLAFFSMCTPAKKNGGNSVDPGGKFDFQVQYLYSGE
                     TFYDTLIIRDRTMVRKYFNDDGKKCAKAQRSPCWVESELESATATLTAEELASLKSLV
                     EETNFMVSDPDPHGLTGKGRFYAYIITVNHKSVEHRVVMGEFPGSADAPPASRRIYLR
                     LLDLAKAKSLGN"
     gene            complement(3911..4207)
                     /locus_tag="PLG78_12660"
     CDS             complement(3911..4207)
                     /locus_tag="PLG78_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24017.1"
                     /translation="MAEYLAAAPVVLITDAEEGRCYFCGSDLGNASCLRSDGIWLWEN
                     GLDHYVLEHAIRLPDTFVAHIEEREFRPPAEVHDDFEFLPMPTGAEVQGLLHLE"
BASE COUNT         1143 a         1176 c         1197 g         1114 t
ORIGIN      
        1 caaggcgaca atgcttttga attgaagaat gcaatggcca aagagatcga ccagcatctt
       61 agagcgctca ttgacattgc caatggccgc gatgctgtgg ggcgtcccat gttcggcggc
      121 gacattgtag agcgcatgcc gtttgaacca gtggtctcca acattcgcgg aggcaagggc
      181 atcgagttag acaacatgat cgtgggagtt gagtaccgcg gaaacattgg ccagcaactt
      241 cgtgaagtgg aacgctctca gtacattgat gtaaatttgc ccggaaacaa agttttctgg
      301 ggaacgaaca tgactgtgac cggctccgtt gacaattccg gctatcgggc caccacagat
      361 caggtcttta agatcgacgg acaaacgatt cgtgtcgctg ccggagatac gatagacgac
      421 atcatcgaca aaatcaacaa cgcaagcctg gaagtgaaag cttctaaaat tggacaggac
      481 tttgcatctc ttcatactac caatcctcac caggtctggc tggaagacat agaaggcgga
      541 actgttcttc gcgacattgg cctgatcaat ccggacaaag ccaatccgcc caacaacttc
      601 gcagagactg cgcgtgtttc ggggttgagc gtatttgatg tattgatcaa acttcgcaac
      661 gacatgattg caggtgacca gctggaaatt tcagggcgtg atcttggaaa cctggatgaa
      721 ggactcaata acattctgcg ttatcgcgcg gaagtaggtg cgcgccagaa tcgtatggac
      781 gaacacgaaa agcgcgttgt atgggatcag acttatatga cggaactgtt gtccaagagc
      841 gagggcgtgg atatgcccga agcgattgtc aatctgaagt atctcgaagc ggttcatcag
      901 tatgcgttga acattggagc tcgcttgatg aagccgactc tgatggattt catgagataa
      961 ggaatttaga atgacaaaga ccggacgcac actcgaaacc aaagcactcg ggaagattac
     1021 tctggatccc gaacaactgt tgagttttcc cgaagggctg tacggttttc gcgactttca
     1081 tgaattcgcg ctcgtagaag aaagtgcaga aaaccctttt aaatggctgc aatctacgtc
     1141 tgatccggca ctggcattca tcgtcattca acctgaattg atcctgagtg gctacagacc
     1201 ggaagtggct cgcgaagatt tgcagtcgat tggactatcc ggcgtagatg aatcgcttgt
     1261 gttcttgatc gtaacgattc ctgaaaacga tccggaaagc atgactgcca atttgcaggg
     1321 tcccgttcta ataaacaaga ccaccaaaca gggccgtcag gcgatctcca tgaacgaaaa
     1381 gcatgtggtt cgccttcctg tcctttcgca ggtggatgcc tgatgcttgt tcttgcaaga
     1441 aagataaacg aatccatcat gataggtgac gacattgaga tcgtcgtcgt ggatatcaaa
     1501 ggggaccaga tcaagcttgg aattcgcgcg ccgcgcagcg tttccgttca tcgagctgag
     1561 atattcaagg aaatccagga gcagaacaag aaggcagtgg aaagtgcgcc gcaatcactt
     1621 ggcaaactca agaacctcct ggacaagaag aaacaatagt cgccatacgg tctgaggtct
     1681 cttgcgcgcc gtccttttca tcttcttgtc ctgtttctcc gcgacgctgg gcgcagagcc
     1741 agacggatcg aagatggacg cgcatacttc catcaactat cgcattacaa atagaaagta
     1801 cgtaagccat atcttattgg aaaggtctgg cggaatcaac tatgtaactc tcctgacaac
     1861 gcatcccatg cagtgggcat ttcactctga atcgcacgcg ctgaaatatg cgtgcggcaa
     1921 gattcctgat tgcatggact tgatgagtcg cctggatgaa tgtttgaacg caggtaagaa
     1981 tctaaatctt cacctggacg gtgaacgaat cattgaaatc caggtttacg agtagcgcgt
     2041 cacttcaggc agcgcgtcca ggtatctatc tcttcatccc aaatcgcaat ctgcaacgct
     2101 atcgtcagta cgtccaggat cattgtgaat ggatagagag gtaacaggta gtaacgattc
     2161 ttttcccgcg acatggacac atagggtacg tagatcaatg cggtctctga acctgctggc
     2221 cgaactccat caaagagctt gagtgccatg ttaccatctg gccggataaa gtagatgccc
     2281 accgctccag agagttgcct caaaggttgt tccttccatt gagttgagtt ttctgccttt
     2341 ccagatgcaa tcggcacgaa aggtgtttcc cgggttccgt tgtagatgcc aaattctccg
     2401 ggacctgcgc gacgcagttc gagaacggcc ccgttctcaa gagttgcgcg aattctggtt
     2461 tgattttcgt ctgtttcaag tttcgctacg cgggcagccg gaacatctgc cgcaacaggc
     2521 ctggacttca ttgcggtcat ggatacactt tgagtggaaa aaaatagcgt gccggcggtt
     2581 tcaaaggagc ctgttggacc cttcgccttg cagccgtccg tgatgttcgt ctcccacact
     2641 ttctgagttt cgaaaatgct caacgtttcg gatgacattg cgtagctgcg actggaatcg
     2701 cctgttactt tgatctgtct ggttgtgcgt ggagattgag cgcagaagac gaacgaattt
     2761 ccggaagctt caaggtcgtg gatgtcctga gcatgctcga aatcgtaacc atccggaaat
     2821 gccatcgtgt gcaaagcggt cgtgatgcat gcagtctggt gcagtgtgac cgctagaatg
     2881 gcggcattga gcagtctcat gatgtctcaa cgagagtctt gctcgtgccc tgtcaatgca
     2941 caatcgattg cttgcctgcc gcgagtggtg tggctttctt gcacatatgc gaaacctttt
     3001 catcctggcg gtgcttgctt ttttctccat gtgcactccc gcgaagaaga acggaggtaa
     3061 ctcagtagat ccgggtggga agtttgattt tcaggtgcag tatctttact caggcgaaac
     3121 gttctacgac accttgatca ttcgcgatcg taccatggta cgcaagtatt tcaacgatga
     3181 cgggaagaag tgcgcgaaag cgcaaagatc accgtgctgg gtggaatcag agctcgaaag
     3241 tgcaacggca actctgactg cagaagaatt ggcctcgttg aagagtctgg tagaggaaac
     3301 caatttcatg gtttctgatc cggacccaca cggtcttact ggcaagggcc ggttctacgc
     3361 atacatcatc acagtgaacc ataagagcgt agagcatcgc gtagttatgg gggagtttcc
     3421 aggttcagca gacgcgcctc ctgcatcgcg acgaatctat cttcgcctcc tggatcttgc
     3481 caaagcaaag tccctcggga attaatcgcc tcgtgcggcc tccgcgcgga gaatccgccg
     3541 caggtgccgg gatacagtcc atcctcgcgc gcgctgttgc gacgctcgga cgctccgaac
     3601 gccgctgtgg accgcacccc ggcacaacgg cataagccgc gctggacctg cgagcctttc
     3661 attttgcgcg cgcggaccac gctccttcag gcttcgcgtt cgcttcggtc tcgctttgcg
     3721 agactgcttc gcaccttgcg gatcgttggc cggaagttgt tcgagtgcca acaatgccgt
     3781 gtaacgtcag gctaagagga gcacatatcc gcctgtggcg gatttgaata gatgggcgac
     3841 gtggagtttg ttgcgaggaa cataagtcct tctgcgacgc cgcttcatcc gaaaacttgc
     3901 ccgattgtcc ttactccaaa tgcaataacc cttgtacctc agcgccggtc ggcatcggca
     3961 gaaactcgaa atcatcgtga acctcggcag gaggccgaaa ctcccgttct tcaatatgcg
     4021 ccacaaaagt atcgggcaac cgaatagcgt gctccagaac gtagtgatcc aatccgtttt
     4081 cccagagcca aatcccgtca gacctcaagc aactcgcatt gccaagatcg ctgccgcaaa
     4141 aataacagcg tccttcctct gcatctgtaa tcaatacaac aggtgccgca gctagatatt
     4201 ccgccaagcg aacgcggtcc gtgtagaccc acgttttgtc cacgaatgct agccagtggg
     4261 gcgcgctctt tccgcaccgt tctggtaaga acggggcaaa ccatttgtag ggccagtcta
     4321 tgatccagtt cattaatcac ctactcgggc atggaactaa tccactttcc acgtttgctc
     4381 agatccagcc acggtttccc ttttagtcga gtttattggc ccacgtcttt tcatttggcg
     4441 ccatgatggg acattcaact ggttggccaa cttctgcgca aaaccattct gaagctttcc
     4501 agtctcacat gaaagcaatt gtatcgctgt tccttcatag tcagctcgat tcttgggtag
     4561 ttgcgcgagg tacaaactac ggccaaacaa tgcaccgcgt atcgtcagat tccattgact
     4621 cacgagacta
//
LOCUS       DAOQJL010000299         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11055_length_4626_cov_4.649311, whole genome shotgun sequence.
ACCESSION   DAOQJL010000299 DAOQJL010000000
VERSION     DAOQJL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11055_length_4626_cov_4.649311"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..722
                     /gene="mazG"
                     /locus_tag="PLG78_12665"
     CDS             3..722
                     /gene="mazG"
                     /locus_tag="PLG78_12665"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNE24018.1"
                     /translation="MDIMARLRGEGGCNWDRAQDAESMRVYLLEEAYEVIEAVNRNDP
                     ALIREELGDLLFIVFFYSRLFEEKGVFSVQDVMRDVSDKLVRRHPHVFGEQKDHSVQE
                     VLQNWEKIKAKEKSSEREAFLPALLRAFKVQEKAARKGFDWENARDVLPHLESEVAEF
                     RQAVADGESSARREEEIGDILFTIVNCLRHLGIHPETALHRSTDKFQDRFARMTESAG
                     EGFMDLSLEEKELLWQKSKQK"
     gene            725..1750
                     /locus_tag="PLG78_12670"
     CDS             725..1750
                     /locus_tag="PLG78_12670"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNE24019.1"
                     /translation="MGPYTTIHLGGPADFFLEANSDQEIIQGLAFAEDKNLRVRILGG
                     GSNVIFSDAGFRGLVIRVATRGIELERETNHGSELRVQAGHEWDAFVEHCVRANLSGV
                     ECLSGIPGTAGGVPIQNVGAYGQEAKETIVSVRVYDRNAKSIISIPGTECEFGYRTSR
                     FKHREAGRFIILDVLFALRNDSSPPRYPELITKMQDPAISGISPHPLAVRTAVLSLRK
                     AKSMVHSAEDPDSISCGSFFTNPVLDAESLAKLEAKMKSSGLTMPRAYPDGHNFKVSA
                     AFLVENAGFHKGLVRGGAGVSTKHALALINRGGTAADLLSLADEIKGEVLSRFGIQLE
                     MEPEYIV"
     gene            1786..2298
                     /locus_tag="PLG78_12675"
     CDS             1786..2298
                     /locus_tag="PLG78_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNE24020.1"
                     /translation="MDYSGGIFNVEEIELVFPKFNNEAKMTESEICMKCHGCCTYVAV
                     PLEYPRSKDTLDTYVWYLLHRNVEIEIDNYNKWFLIFKTPCDKLLPNGACSIYETRPE
                     ICRDYSADNCSRTAKDHAHQFKQPSELLQYLEQKKKAKSGTVKPASASRRIAGKKSVR
                     MTGKKSALRK"
     gene            2295..2960
                     /locus_tag="PLG78_12680"
     CDS             2295..2960
                     /locus_tag="PLG78_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE24021.1"
                     /translation="MKPFWISIGVKLLIEFWFLFIRAKIIVPPETEKLLAEKRGFILA
                     GFHNQIMSLTYHCSKYLGKKRKIKLTPLVSQSKDGEMIYQTFLRFQMYSERGSTSKGG
                     AAAFRALLKRIKDGWVPIFTPDGPRGPLYKVQPGVISLAAATGLPIVHFFSVFDRAYQ
                     TRSWDKMRLPKFGAKQWIEYSQPFYVPRKLSEEELEETIGRLEREMMQQVDRLETLVT
                     SKT"
     gene            complement(2944..3531)
                     /locus_tag="PLG78_12685"
     CDS             complement(2944..3531)
                     /locus_tag="PLG78_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNE24022.1"
                     /translation="MEESQKSPEEKARDAKDQLNNWVAVAVALIATFTGITKIKDDNI
                     VQAMLQMKSDAVDRWNESQASKIKLHIAENGLRQTQIASLTAGGAQRQALNTAAKEYE
                     AAIEKYRKRAEEASAEAKKNEEAYDSLGYRDDQFDMSDMFLSIAMSLLAVSALTGKRW
                     LFYFSMVLSTFGVIMGVAAFVQLHIHPNFLVKFLT"
     gene            complement(3564..3968)
                     /locus_tag="PLG78_12690"
     CDS             complement(3564..3968)
                     /locus_tag="PLG78_12690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE24023.1"
                     /translation="MNLDDLSRLESLCFPDPWTREMLEGSVARPDSIVIFRKINEQIS
                     AYLLATSSENGIELLRIGVAPSGRRLGLAKSMIMELVAMSQRILLEVSDSNRAAISLY
                     LGCGFKEIARRKRYYADGSDCIVMQLEKRSGF"
     gene            4025..>4626
                     /locus_tag="PLG78_12695"
     CDS             4025..>4626
                     /locus_tag="PLG78_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNE24024.1"
                     /translation="MERFILASCLPYRSAIYRMGGFQAFPNDFLKFYTKEEIETARID
                     ASRALEILEKDKDLRFISILDERYPASLRNIYDPPPGLYICGADLDGEFDAIVGTRAP
                     SPVVRCAVRILVRSLSSMIVSGFARGVDAEAHLECARGGRRTIAVLGSGHRFAGPGST
                     LRLLRPYSDFTTFVSEFPPHWPARSHSFPRRNRIIAGLSA"
BASE COUNT         1160 a         1109 c         1167 g         1190 t
ORIGIN      
        1 tactggacat catggcccgc ctgcgcggcg agggcggatg caactgggat cgcgcccagg
       61 atgcggagtc tatgcgcgtc tatttgctcg aagaagcata tgaagtcatt gaggcagtca
      121 acagaaacga tcccgctctg attcgcgaag aactgggaga tttgctcttc attgtttttt
      181 tctattcgcg ccttttcgaa gagaagggag tcttttcagt tcaggatgtg atgcgcgacg
      241 tttcagataa gctcgttcgc agacatcctc atgtttttgg tgagcaaaag gatcatagcg
      301 ttcaggaagt acttcaaaac tgggaaaaaa tcaaggcaaa agaaaaatcc tctgaaagag
      361 aggccttttt gcccgcactt ctgcgtgcgt tcaaagtgca ggagaaagca gcgcgcaaag
      421 gtttcgactg ggaaaacgca agagatgttc ttccgcatct agaatctgaa gtggcggagt
      481 ttcgtcaggc agttgcagac ggagaaagtt ctgccaggcg cgaagaggaa atcggggata
      541 tattgtttac aatcgtaaac tgtttgcggc atttaggaat tcacccggaa acggctcttc
      601 acagatccac ggataaattt caggatcgtt ttgcgcgaat gacagaatcc gcaggcgaag
      661 gattcatgga tctcagttta gaggaaaagg aattactctg gcagaaatca aaacaaaaat
      721 agatctgggc ccttacacca cgatccatct gggcggaccc gcagattttt ttctagaggc
      781 aaactcagac caggaaataa tccaggggct tgcgtttgca gaagataaga acttgcgagt
      841 gcgaattctc ggcggtggtt ccaatgtgat tttctcggac gctggatttc gcggacttgt
      901 aattcgcgtg gccactcgcg gaattgaact ggaaagggaa accaatcacg ggtctgaact
      961 tagagtccag gcggggcacg aatgggacgc attcgtagag cattgtgtgc gagccaacct
     1021 ttccggtgta gagtgtctct ccggcattcc gggaacagcg ggtggagtgc ccattcagaa
     1081 tgtgggagcc tacggacagg aagcaaaaga gacgatcgtt tctgttcgag tttatgatag
     1141 aaatgcgaag agcattattt ctattccggg cacagagtgt gagtttggtt acagaaccag
     1201 tcgctttaag cacagagaag cagggcgatt catcattctg gatgtactct ttgccttacg
     1261 aaatgattcc agtccgcccc gttatccgga attgatcacc aaaatgcagg atccagcaat
     1321 ctctgggatt tcgcctcatc ctctggccgt gcgcacggct gtgctatcac tcaggaaagc
     1381 aaagtccatg gttcattctg cagaagatcc ggattccatt tcctgcggct ccttcttcac
     1441 caatccagtc ctggatgcag agagtctggc taaactcgag gcaaaaatga agtccagcgg
     1501 cctgactatg ccgcgggcgt acccggacgg acacaacttc aaagtatccg ctgcgtttct
     1561 tgtggagaat gctggatttc acaaaggact cgtgcgtggg ggtgcgggcg tttccacaaa
     1621 gcacgctctt gctcttatca atcgaggcgg aacagcggcc gatctacttt cgcttgcaga
     1681 tgaaatcaaa ggagaagtgc tatcacgctt tggaattcaa ctcgagatgg aacctgaata
     1741 catcgtttga ttgcctccct gagcgtttag aatttcctgg cccggatgga ttattccggt
     1801 ggcattttta atgtagaaga gatcgagctc gtctttccga agttcaacaa cgaagcaaaa
     1861 atgacggaga gcgaaatatg catgaagtgc cacggttgtt gcacgtacgt tgctgttcct
     1921 ctcgaatatc caagatcaaa agatactctg gatacatacg tctggtactt gctacacaga
     1981 aacgtagaaa tagaaatcga caactacaat aaatggtttc tgatctttaa gactccttgc
     2041 gataagctcc taccgaacgg cgcatgcagc atttacgaaa cgcgcccgga gatatgccgg
     2101 gattattcag cagacaattg ctctcgcacc gcgaaagatc atgcacatca attcaagcaa
     2161 ccatcggaac ttctacagta tctggagcaa aagaagaaag caaagtcggg gacggtaaag
     2221 cctgcatccg cttcgcgcag aattgccggt aaaaagagtg ttcggatgac tggaaagaaa
     2281 tcagcattgc gcaaatgaag cctttctgga ttagcattgg cgtaaaactg ctgatagagt
     2341 tctggttctt gttcattcgt gcgaagatca tagtcccacc cgaaacagaa aagctcctcg
     2401 cggagaaacg cggattcatt ctggccggat ttcacaatca aatcatgtct ttgacgtatc
     2461 attgttccaa atatcttgga aagaagcgca agatcaaact tactcctctg gtttcgcaat
     2521 caaaggacgg agaaatgatt tatcagacgt ttctgcgctt tcaaatgtat tcagagcgag
     2581 gctctacgag caaaggcgga gcggcagcgt ttcgcgctct gctcaaacga atcaaggatg
     2641 gctgggtgcc gattttcact ccggatggcc ccagaggccc gctctacaaa gtacaacctg
     2701 gagtcatttc tctggcggcg gctaccggtc tccccatcgt tcatttcttt tccgtatttg
     2761 atcgcgcgta tcaaacaaga agctgggata agatgaggct gccgaagttc ggcgcgaagc
     2821 agtggattga atattcacag cccttctatg tgccgcgaaa gctgagcgaa gaagagcttg
     2881 aggaaacgat cggccgtctg gaacgcgaaa tgatgcagca ggtcgatcgc ctcgaaactc
     2941 tcgttacgtc aaaaacttga ccaggaaatt cgggtgaatg tgcagttgca caaaagccgc
     3001 cactcccatg atcactccaa acgtacttag aaccatggaa aaataaaaga gccagcgttt
     3061 tccagtcagt gcggagacgg ccagtagaga catggcaatc gaaagaaaca tatctgacat
     3121 gtcgaattga tcatctctgt atccgagcga atcgtatgct tcttcgtttt tctttgcttc
     3181 tgcgcttgct tcctctgcgc gttttctgta tttctctatg gcggcttcgt attcttttgc
     3241 agctgtattc agagcctgtc tttgtgcgcc tccggctgtc agagatgcga tttgagtctg
     3301 cctgaggccg ttttccgcaa tgtgaagttt gattttagat gcctgcgatt cattccatct
     3361 gtccacagcg tctgatttca tttgcagcat tgcctgcact atgttgtcgt ccttgatctt
     3421 ggtaattccg gtaaacgtgg caatgagagc cacggcgacg gccacccagt tgttgagctg
     3481 atctttcgcg tcccgtgctt tctcctctgg cgacttttgt gactcttcca tagaaactcc
     3541 ttgcgggagt tacgattgag cattcaaaaa ccggaccttt tttctagctg catgacaatg
     3601 caatcggacc cgtctgcgta gtaccgtttt cttcgcgcaa tttccttaaa tccacaacca
     3661 aggtagaggg agatggcagc gcggtttgaa tcagacactt cgagtagaat cctctgcgac
     3721 atggccacta gctccatgat cattgatttt gcaaggccca gccttcttcc tgaaggggca
     3781 acgccgattc gcagtaactc gattccgttc tccgaggatg tggccagcaa atacgcactg
     3841 atttgctcat ttattttccg aaatattacg atgctgtctg gcctggccac gcttccttcg
     3901 agcatctctc tggtccaggg atccgggaag caaagcgatt cgagccggct gagatcatcc
     3961 aggttcacgt gtcctcgtac ctgttttttt cgaaacgcac cattcttttt ttaccataga
     4021 aaagatggaa cgattcatac tggccagctg ccttccttat cgatctgcca tttatcgcat
     4081 gggcggattt caggcgtttc cgaatgattt tctgaagttt tatacaaagg aagaaatcga
     4141 aacagcaagg atagatgcgt cgcgtgccct ggagattcta gaaaaagaca aagatcttcg
     4201 ctttatttcg attctggatg aacgttatcc ggcatctcta cgcaacatct atgatccgcc
     4261 tcctggattg tatatttgtg gagcagatct tgacggcgag tttgatgcga tagtcggcac
     4321 gagagctccg tccccggtcg tgcgatgcgc cgtgcgaatt ttagttagat ctctctcttc
     4381 catgattgtt tctggttttg ccagaggcgt ggatgcagag gcgcatctgg aatgcgcgcg
     4441 cggaggcaga cgaaccatcg cagttctcgg gtccggtcac agattcgccg gacctggctc
     4501 cacccttcgc ctccttcgtc cgtacagtga cttcactaca tttgtttctg aatttccgcc
     4561 gcactggccc gcaagatcgc acagttttcc caggagaaat cgaatcatcg ccggactttc
     4621 tgccag
//
LOCUS       DAOQJL010000300         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11059_length_4625_cov_5.663239, whole genome shotgun sequence.
ACCESSION   DAOQJL010000300 DAOQJL010000000
VERSION     DAOQJL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11059_length_4625_cov_5.663239"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLG78_12700"
     CDS             <1..673
                     /locus_tag="PLG78_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24025.1"
                     /translation="PKLLSHPWDLVFFPFRLEFPGAMPALLGGGWGGLLIRLYLYWMF
                     GSIVEAELGRVRYNVYVWMGVVMVTLTGVFIPEVTAVYIYYSVFFAVAYLAPNMELYI
                     MFILPVKIKWLAWLAVAGIVLESLGSAAATGNYWLLAGPFLTFLNFFIFFGREFLETK
                     VRAHSSGRRMKAMATVRVSALHRCTVCGMTENDDPNMEFRFCVDCSDHEYCMTHLRQH
                     EHIRE"
     gene            complement(678..1544)
                     /locus_tag="PLG78_12705"
     CDS             complement(678..1544)
                     /locus_tag="PLG78_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE24026.1"
                     /translation="MAKKVLGRGLSNLLPGESTIQIQTNPDYQELDISIVVPSPDQPR
                     KQFNPDDIRELAQTLHSVGLIEPIVVRKSGANFEIISGERRFRACKLAGFKKIPAVIK
                     QVDDIRALEMGLIENIQREDLNPMEEARAYEIWMEKTGDKPSDLAEKVGKDRTTITNL
                     TRLLKLPEDIQKLIENKMMTAGQARPLLAIADRRTSTKLADRICRENWSARRVEDEVA
                     KLQGEPGARSPVAKAAARDPNLKSVEAKLRTKLGAKVSLNHKKNGAGKLTVFYGNLDD
                     LDRILHGMGIKA"
     gene            complement(1537..2298)
                     /locus_tag="PLG78_12710"
     CDS             complement(1537..2298)
                     /locus_tag="PLG78_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE24027.1"
                     /translation="MERIYAVANQKGGVGKTTTAVNLGGYLARMGHNVLLVDMDPQGN
                     ASSGLGVDIRELPRSVYSMLIGELSASEILQPTHIKGLSILPSNTDLAGIEPDLLQTD
                     NREYVLKRALEQLKTRYEIVLIDCPPNLGILTLNALCAASRVLIPLQTEYYALEGLTQ
                     LLDAISRVQKNFNPTLILDGVVLTMYDSRTSMSQQVVEDVRSHFHDRVYQAVIPRNVK
                     LSEAPSFGQFIGDYAPDSTGAAAYQALAKEVYDRG"
     gene            complement(2277..2987)
                     /locus_tag="PLG78_12715"
     CDS             complement(2277..2987)
                     /locus_tag="PLG78_12715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE24028.1"
                     /translation="MLEEFAKAGLPIPERLIEIVDWDRLGRFCEFLRNKNEAGGFFSK
                     GDAERILERHLYENVIFMDYVVSRCKVSRETNLADAGSGPGLPGYLFACLKQPPRITL
                     IDSSRRRLGFVEEHHRAEGSPASVRFEFSRLEEMRTTFSLIVMRALIPFPQCVELVCH
                     LVGKGGFLVLSGSRFQETDLSRFGFVSRETYVPSELSFLGARTFLLLEKTGKQDSLYP
                     RPWKIIQQEVKEWKESTP"
     gene            3052..3765
                     /locus_tag="PLG78_12720"
     CDS             3052..3765
                     /locus_tag="PLG78_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24029.1"
                     /translation="MRTLLLLSLAIPAALHAFQPGRYHPMDSYVTAGSPGGPASMVAK
                     DAKGQTLETVQFTYDGSGKLIRESFVDSKGQPAGKAEYKRNGDQLTEELRFDRTGTLL
                     SRTVFKYKDKDLIAMEVYNSQNALELRRTYTIQGGRITGGDEISDGVTDHFVMKYNDV
                     ARLLEALVFVQQDGHPFGHIAYRYDAQKRITERERVQVDKRELCRYEYDGAGRIIRFS
                     YFVMQGDWRLQKTLTFTYP"
     gene            3774..4490
                     /locus_tag="PLG78_12725"
     CDS             3774..4490
                     /locus_tag="PLG78_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNE24030.1"
                     /translation="MHEHRPYKYFDLVMAGFVTVLLCSSLIGVSKVSEFPEIHLGFLT
                     IGPFSFGSAVLFFPFSYIFGDILTEVYGYARARRVVWAGFGALFFATFMSWGVLAMPA
                     APGWPHQAAYETVFGSTWRIVLASLTAFWAGEFVNSFVLAKMKIWTEGKLLWARTIGS
                     TIAGEAVDSLIFYPVAFLGVWDTALVQKVLISNFFLKVGWEALMTPITYAAVSFLKRA
                     EHEDYYDRDTNFSPFRLDDE"
BASE COUNT          997 a         1158 c         1221 g         1249 t
ORIGIN      
        1 acccaaattg ctctctcatc cgtgggacct agtctttttt ccgtttcggc ttgagtttcc
       61 aggcgcgatg ccggccttgc taggcggtgg atggggaggc ctgttgattc ggttgtatct
      121 gtactggatg tttggatcca ttgtggaagc ggaactcggc cgggtcagat acaacgtcta
      181 cgtgtggatg ggcgtcgtca tggtgacgct gaccggggta ttcattccgg aagtaacagc
      241 tgtctacatc tattattcgg tgttctttgc ggtggcatat ctcgcgccca acatggagct
      301 ctatatcatg ttcatattgc ctgtgaagat caaatggctt gcgtggctgg cagtggcggg
      361 cattgtcctg gaatcgcttg gaagtgccgc ggccaccggg aactactggc ttcttgctgg
      421 cccatttctc acattcttaa atttctttat attctttggc agagagtttc ttgagaccaa
      481 ggtccgcgca cattcttctg gtcgtcgcat gaaggcaatg gcgaccgtgc gagtgagtgc
      541 acttcatcgg tgcactgtgt gcggcatgac agagaacgat gatccgaaca tggagttcag
      601 gttctgcgtg gattgttcag accatgagta ctgcatgact catctgcgac agcatgagca
      661 catacgagaa taagatccta tgctttgatt cccatcccgt gcagaatacg atccaggtcg
      721 tccaggtttc catagaatac agttaacttg cctgcaccgt tctttttgtg gttgaggctc
      781 accttcgctc ccagtttggt tctgagtttt gcctctacgg atttcaaatt tggatcgcgt
      841 gccgcggctt ttgctacagg ggatcgtgcg ccaggttcac cctggagttt tgcgacttca
      901 tcttccactc gtctggcaga ccagttctct ctgcaaatgc gatctgcaag ttttgtcgag
      961 gtgcgtctgt ctgcgatggc aagcaaggga cgagcctggc cggcagtcat catcttgttc
     1021 tctatgagtt tctggatgtc ttcggggagt ttcagaagcc gcgtgagatt ggtgatcgtc
     1081 gttcgatctt tcccaacttt ctccgcaaga tcggatggct tgtctccggt cttctccatc
     1141 cagatttcgt atgcgcgggc ttcttccata ggattgagat cttctctctg gatgttctcg
     1201 atgaggccca tctctagagc gcggatgtca tccacttgtt tgatgactgc ggggatcttt
     1261 ttaaatcctg cgagtttaca tgcgcggaac cgtcgctcac ccgagatgat ctcgaagttg
     1321 gcgcctgatt ttctgactac tatgggttca atgagaccga cgctatgcag ggtctgagcg
     1381 agctcgcgaa tatcgtctgg attgaattgc ttacgcggtt gatccggaga tggaacaaca
     1441 atggagatat ccagttcctg ataatcaggg ttggtctgga tttgaatggt ggattcgccg
     1501 ggcagaagat tggatagtcc gcgccccagt acttttttag ccacgatcgt atacctcctt
     1561 tgcaagtgcc tgataggctg ccgcgcccgt tgagtctggc gcatagtctc caatgaattg
     1621 tccgaatgaa ggagcttccg agagctttac gtttctcgga atgactgcct ggtagacgcg
     1681 atcatggaaa tgagagcgaa cgtcttcgac cacctgttgt gacatggacg ttcgagaatc
     1741 atacattgta agaaccacac cgtccagaat gagggtgggg ttgaagtttt tttgcactct
     1801 agaaatagca tcgagcaatt gagtgagtcc ttcaagagcg tagtattccg tctgcaatgg
     1861 aatcagtact ctacttgcgg cgcagagtgc gttgagggtg aggatgccca ggttaggcgg
     1921 acagtctatg agcacgattt catatctggt cttcagttgt tcgagagctc gcttgagcac
     1981 atactctctg ttgtccgttt gcagaagatc cggttcaatt cctgcaaggt ccgtgttgga
     2041 cggcaagatg gagaggcctt tgatgtgagt tggttgcagt atttctgaag cggataattc
     2101 cccgatcagc atgctgtaga cggagcgcgg aagttctctg atgtctactc cgagcccgga
     2161 ggatgcattc ccctgagggt ccatgtccac gaggaggaca ttatgtccca ttcgcgccag
     2221 gtatccaccg aggttcacag cggtcgtagt tttgccaact ccgcccttct gatttgctac
     2281 ggcgtagatt ctttccattc cttcacttcc tgttggatga ttttccaggg cctggggtac
     2341 aggctatcct gtttccctgt tttttccagg agcaggaacg ttctggctcc gagaaaagac
     2401 agctcagatg ggacataagt ttcacgtgaa acaaaaccga acctggacag gtccgtttcc
     2461 tgaaatcttg atcccgagag cactagaaat cctccctttc ccacaaggtg gcacaccagc
     2521 tcaacgcact gcggaaacgg aatgagtgcg cgcataacaa tcaggctgaa cgtggttcgc
     2581 atttcttcca ggcggctgaa ctcgaaacga acggaagccg ggctcccctc tgccctgtga
     2641 tgctcctcaa caaagcccaa tcttcgcctg gaggaatcaa tgagcgtaat ccgcggtggc
     2701 tgttttaaac acgcaaagag atatccaggt aggccagggc ccgaaccagc atctgccaga
     2761 ttagtttcac gtgaaacctt gcatcgtgat acgacataat ccatgaatat gacgttttcg
     2821 tacagatggc gctccagtat tctctccgca tctcccttcg aaaagaatcc ccctgcttca
     2881 ttcttgtttc tgagaaattc acagaatcgt ccaagtcgat cccagtccac gatctcaatg
     2941 agccgttccg ggatcgggag gccggctttt gcaaattctt cgagcatgca agcatccttc
     3001 ttacctccgg ttttcggtca actccagcgc ccttcctccg atacataagt ggtgagaaca
     3061 cttctgctcc tgagcctggc gatcccggct gccctccatg cctttcagcc agggcgctat
     3121 catcctatgg attcctatgt cactgcggga tctccaggcg gcccggcgtc gatggtcgcg
     3181 aaagacgcga aaggccagac tctcgaaacg gttcaattca cttatgatgg ttccggaaag
     3241 ctcatccgcg aatcatttgt agattcgaaa ggtcaacctg ccggtaaggc ggagtataaa
     3301 cgcaatggcg accagctgac agaggaactt cgatttgatc gcacgggcac tctgctgtcg
     3361 cgcacagtct tcaaatacaa agacaaagac ctcatcgcga tggaagttta caactcccaa
     3421 aacgctctgg aactcaggcg tacctacaca atccagggtg ggagaattac aggcggtgat
     3481 gaaatttcag acggagtcac ggatcacttc gtgatgaagt acaacgatgt agcccgactc
     3541 ctggaagctc tcgtatttgt tcaacaagac ggtcatccgt tcggtcacat cgcgtatcgt
     3601 tacgatgcgc aaaagcggat cacggaacgc gagagagtgc aggtcgacaa aagggaattg
     3661 tgtcgatatg aatatgacgg cgcgggacga atcattcgct tctcatactt cgtgatgcaa
     3721 ggagattggc gccttcagaa gactttgaca tttacttatc cctgaggcac cacatgcacg
     3781 aacaccggcc ctacaaatat ttcgatctag tcatggccgg ttttgttacc gtgcttcttt
     3841 gttccagttt gattggcgta tccaaggttt ccgaatttcc ggagatccat ctgggcttcc
     3901 tgacgattgg tcctttcagc tttggctcgg ccgttctttt ttttccattt agctacatct
     3961 ttggagacat actcacagaa gtatatggct atgcgcgggc gcgcagagta gtatgggccg
     4021 ggtttggagc tctgttcttt gcaacattca tgagctgggg agtgctggca atgcctgcgg
     4081 cgccagggtg gcctcatcag gcagcatacg aaacagtctt cgggtccaca tggcgaattg
     4141 tgctcgcaag tcttaccgca ttctgggccg gcgagtttgt gaactcgttc gttctcgcaa
     4201 agatgaagat ctggacggaa ggcaaattgc tctgggctcg taccatcggt tctacgattg
     4261 ccggagaagc agtggactct ctgattttct acccggtggc cttcctcgga gtatgggaca
     4321 cagctctagt acagaaggtt ttgatttcca atttcttctt aaaggttgga tgggaagccc
     4381 tcatgactcc tatcacgtat gctgctgttt cttttttgaa gcgagcggag cacgaggact
     4441 attacgatcg ggacaccaat tttagtccgt ttcgtctgga cgacgagtag cgcgggtttc
     4501 cccgcgccct ctggttcgcg taactggcgt taggcgaacg agttgctctg tggcttagtg
     4561 aaagcctcct gttttcattt ctgacacgag cctgctgatg gcatccttcg catctccaaa
     4621 gaaca
//
LOCUS       DAOQJL010000301         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11063_length_4624_cov_3.702342, whole genome shotgun sequence.
ACCESSION   DAOQJL010000301 DAOQJL010000000
VERSION     DAOQJL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11063_length_4624_cov_3.702342"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLG78_12730"
     CDS             <1..433
                     /locus_tag="PLG78_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24031.1"
                     /translation="DRRLKTLSVAGVLVILVLQFVLYPLLNKEVIPVGMSGRFASKMC
                     VISTETWDAMDLKAISPGVDVRVVPPGSPVTCIDGERGVLYISDMKQQENMYEEVVAK
                     QGYKESASWIIWKRWLSYFDVVDHWQKGSSLTTRAIYYEKH"
     gene            420..761
                     /locus_tag="PLG78_12735"
     CDS             420..761
                     /locus_tag="PLG78_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich protein"
                     /protein_id="HNE24032.1"
                     /translation="MKNIRSRKAHHLSLIPFYVFCLATLVFAIVSHKQQGDSGADTSG
                     GNGPDCEVICGRFAECLAVVYPPEQVKMYAWTIAAGCRNGCAKQRKRLGECFTPGATC
                     PAIQGCIGSKL"
     gene            complement(768..2654)
                     /locus_tag="PLG78_12740"
     CDS             complement(768..2654)
                     /locus_tag="PLG78_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE24033.1"
                     /translation="MALRTFYDIIDQSAKTWPSQVSFRKRGASGQFPGRTFSEIKRHV
                     DSLTAGFIAEGIQPGDRVTYLCDSSPNWLIGDCAITSTGAVSVPRGTDVTDEDIKYIL
                     SHSESRFALVQREKDKKRLEELKSHFKNLEKVFVLQTEDAELARGKDSVTELMEKGDA
                     LLAKDPELVSKRVAALDPGALATLIYTSGTTGSPKGVMLNQFGWVHAIERVLDRIQFT
                     HNDRAVSLLPPWHAFERAVEYGIVMRGMDFLVSDIQNLRNDLFEFKPTVFPSVPRIWE
                     TVYNGIITKVKKESPASQAIFNFFLSVGSFWARWKAIAFGYDPAVKQPFILWSAIRRI
                     TALLVLLATSPLKLIAMRVFHGIHAALGGHLRMSVSGGSALPGVVDQFLTAIGITVLE
                     GYGMTETSAVISIRRLNKPTPGTVGTPIPGYEVCLKDDKGNLVTEIGQKGSLWVKSKQ
                     ILMGYYKRPELNQSIFDKNGFFDTGDIMRLNHRGELSFAGRAKDTIALGGGENVEPVP
                     IEDRLITSEFIDQVMVVGDDKKSLGALIVPHFDHVRKKLPGIPDKTELWNFDKSVRDL
                     FKGEITRLISRESGFKAFEVIPGSCFYIVPRQFDPDTEMTRTLKLKRNVIKDNFAQDI
                     DAMY"
     gene            complement(2717..3568)
                     /locus_tag="PLG78_12745"
     CDS             complement(2717..3568)
                     /locus_tag="PLG78_12745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE24034.1"
                     /translation="MKSEKKFQHKSSYYNDIALRLMQRLFHVEHLHYGYFEGKLKPEI
                     KNLPAAQEAYMKLLLKQIPSGVKKIFDVGCGTGEVAAALTKKGYQMTCIAPDPYLVSM
                     TEKKTGGKAVTFVDLYENIDTEVEHGTFDLILMSESCQYIKPDPGWINHVRFLKPGGY
                     ILVADFFKAKPPDERNPSKSGQPLDDFLRRADENGFKIVKKQDITKNVAPTMDIYQRI
                     ILDRIFPVVEAVFEIVERKAPTIYKILRYFLSAKILKLKDKYEKTGADLFSEYKRYMI
                     FLLQKKK"
     gene            3666..4148
                     /locus_tag="PLG78_12750"
     CDS             3666..4148
                     /locus_tag="PLG78_12750"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNE24035.1"
                     /translation="MQDPRPYRKNVGIVVFNSQGQVLVGERMGMSGSVQMPQGGMDDG
                     EEPLAAALRELEEETGLSLSGPPQFEILNWLSYDFPEDVPDKLKKYRGQMQKWFFFYW
                     DGNPASLSLDHHQQEFSRLFWADFEDVVTRVVAFKQEVYRTLFETGRTFIKQYVAALR
                     "
     gene            4268..>4624
                     /locus_tag="PLG78_12755"
     CDS             4268..>4624
                     /locus_tag="PLG78_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24036.1"
                     /translation="MRVFALVCFLVAGSLCAETLLLKSGARVEGTIISQNRTTVTIRT
                     AGGDQTFRKDAIRRIQYGQTAAEREAEKAAAEKRAAEKAEADRIAAEKAAAEKSEAKV
                     TTPLEKVEEKPEANTKT"
BASE COUNT         1086 a         1097 c         1167 g         1274 t
ORIGIN      
        1 ggatagaaga ctcaaaactc tgagtgtggc cggagtcctg gtcattctgg ttcttcagtt
       61 tgtgctctat ccccttttga acaaagaagt tattcctgtt ggaatgtcag gtaggtttgc
      121 ttctaagatg tgtgtgatca gtacggaaac gtgggatgct atggatctta aagcaatctc
      181 tcctggcgtg gacgtcagag tggttccccc gggaagtccg gtaacatgca tcgatggtga
      241 gaggggagtc ctctacatat cagacatgaa gcagcaagag aacatgtacg aggaagtggt
      301 ggcaaagcag ggatacaaag aatctgcaag ctggatcatc tggaaacgct ggctttccta
      361 ttttgatgtg gtggatcact ggcagaaagg atcttctctc acaaccaggg cgatttatta
      421 tgaaaaacat tagatctcgc aaggctcatc atcttagcct gattcctttt tatgtgtttt
      481 gtctggccac cctcgtgttt gccattgttt cccacaaaca gcagggcgac tcgggcgcag
      541 acacgagtgg cggaaatgga cccgattgtg aagtcatctg cggaagattc gcagagtgtc
      601 ttgctgttgt ttatccacct gaacaggtga agatgtacgc gtggaccatt gccgctggat
      661 gtagaaacgg ctgtgccaag cagcgcaaac gccttggaga gtgctttact ccaggtgcaa
      721 cgtgcccggc tattcagggt tgcattggca gcaagctctg accaatttca atacattgca
      781 tctatgtcct gggcaaagtt gtccttgatt acgtttctct ttaatttgag agtgcgcgtc
      841 atttcagtgt ccgggtcaaa ttgacgcggc acaatataga agcaacttcc cggtattact
      901 tcaaatgctt tgaatccgct ttcgcgcgaa atgaggcgag tgatttcccc tttgaataga
      961 tcgcgcacac ttttatcaaa gttccatagc tccgtcttgt cgggaattcc cggtagtttc
     1021 tttctcacat gatcaaaatg cggaacaatc agcgcgccca ggctcttctt gtcgtctcct
     1081 actaccatga cctggtcaat gaattcagac gttatgagac gatcttcaat cggcaccggc
     1141 tctacgtttt ctccgccgcc cagagcgatt gtatcttttg cacgaccggc gaaggaaagc
     1201 tctcctctgt gattgaggcg catgatgtcc cctgtgtcaa agaacccgtt cttatcaaag
     1261 atagactgat tgagttcagg acgtttgtag taacccatga gaatctgttt gcttttgacc
     1321 cagagactgc ccttctgccc gatttcagtt actaaatttc ctttatcatc tttcaggcac
     1381 acttcgtaac caggaatggg agttcccacg gttcccgggg tgggtttgtt cagccttcga
     1441 atggagatga ccgcggaggt ttctgtcatc ccgtatcctt cgagcactgt aattccgatc
     1501 gcggttagaa attgatccac aacgccgggc aaggcacttc ccccggatac gctcattcgc
     1561 aaatggcctc cgagagccgc gtgaattccg tgaaacactc gcatcgcaat gagcttgagt
     1621 ggcgaagttg caagcaagac cagaagtgca gtgattcttc gaatggctga ccagagtatg
     1681 aagggttgtt tcaccgccgg gtcatatcca aaggcgattg ccttccatct tgcccagaat
     1741 gatccaacag acaaaaagaa attgaaaatc gcctggcttg cggggctttc ttttttcact
     1801 tttgtgatga ttccattgta cacagtttcc cagattctgg gaacagaagg gaatactgtt
     1861 ggtttgaatt caaagagatc atttctcagg ttctgaatgt cgcttaccag aaaatccatt
     1921 cctcgcataa cgatcccgta ttcgactgcg cgctcgaagg catgccacgg cggtaggagg
     1981 ctgacggcgc gatcgttgtg cgtgaactgg attctgtcca ggactcgttc gatggcgtgt
     2041 acccatccaa actgattgag catcactcct ttcggagaac ctgtagtgcc actcgtataa
     2101 atcagcgttg ccagagcacc cggatcgaga gctgctactc tttttgaaac aagctctgga
     2161 tcttttgcca ggagagcatc tcctttttcc atgagttctg ttacgctgtc tttgccgcgg
     2221 gccagctctg cgtcttccgt ttgaagaacg aatactttct ccagattctt gaaatgcgat
     2281 ttgagttctt cgaggcgttt cttgtctttc tccctttgta cgagtgcgaa ccgactttcc
     2341 gaatgcgaaa gtatgtattt gatatcttcg tctgttacgt ccgttccgcg cggcacactc
     2401 acagcccccg tggaagtgat ggcacaatcg ccaatgagcc agttcggcga tgagtcgcac
     2461 aaatacgtaa cgcgatctcc cggttgaatt ccttctgcaa tgaagcccgc agtgaggcta
     2521 tctacatgcc gtttgatttc agagaaagtc ctgccgggga actggccgga tgctccacgt
     2581 ttgcgaaatg aaacctggga aggccaggtt ttcgccgatt ggtctattat gtcgtaaaaa
     2641 gtgcgcagag ccatgctccg cttatgcgct caatcagcga acaatcaact actatttgat
     2701 cgagccggga cagcttctac tttttcttct gcaaaaggaa gatcatgtat ctcttatatt
     2761 ctgagaacag atctgcaccc gtcttctcgt acttatcttt cagtttcaaa attttcgcgg
     2821 atagaaagta gcgaaggatt ttgtaaatgg tgggggcttt cctttccaca atttcgaata
     2881 cggcttctac taccgggaag atacgatcga gaatgattcg ctggtaaatg tccatggtag
     2941 gtgctacatt ttttgtaata tcttgtttct ttactatctt gaatccgttt tcgtctgcgc
     3001 gcctcagaaa gtcgtccagt ggctggccgg atttgctggg gttgcgttca tccggtggct
     3061 ttgctttgaa gaaatccgcg accagaatgt atccacctgg tttaagaaaa cgaacatgat
     3121 tgatccatcc tggatccggt ttgatgtact ggcaggattc tgacatgaga atcaaatcaa
     3181 acgttccgtg ttctacttct gtatcaatgt tttcgtagag gtccacgaac gtgacagcct
     3241 ttcctcctgt ctttttttct gtcatggaaa ccaggtaagg gtccggcgct atgcaagtca
     3301 tttgatagcc cttctttgtg agagcggctg ccacttcacc tgtgccgcag ccaacatcga
     3361 agatcttctt tacgccggac ggaatttgtt tgagcagaag cttcatgtat gcttcttgcg
     3421 ctgccgggag attctttatt tcgggcttca gttttccttc gaagtatccg tagtgaagat
     3481 gctctacgtg aaacaatctc tgcatgagtc gcaaagcgat gtcgttgtag tagctgcttt
     3541 tgtgctggaa cttcttttcg gatttcataa gcaatgttag tgatttgttt tcgcgggtcc
     3601 tgaggcaagc gattctcttg cgcgaaaatg cttgcgcaga tagcctgccg acttgatttc
     3661 tccgtatgca agatccccgg ccgtatcgca aaaatgtcgg catcgtagtc tttaactcgc
     3721 agggccaggt tctcgttggt gaacgcatgg ggatgtcggg gtccgttcag atgccgcagg
     3781 gcgggatgga cgacggggag gaacctctcg cggcagctct tcgcgaattg gaagaggaaa
     3841 caggactttc tctttccggg ccacctcagt tcgaaattct caactggctc tcctatgatt
     3901 tcccagagga tgttccggat aagctgaaga agtaccgggg gcagatgcaa aaatggtttt
     3961 tcttctactg ggacggaaat ccggcttctc tatctttaga ccatcaccag caggagtttt
     4021 cccgcctgtt ctgggccgat ttcgaagatg ttgtcaccag agtggttgca ttcaagcaag
     4081 aggtctacag aaccttgttt gaaacaggcc gcactttcat caaacagtat gtggcggccc
     4141 ttcggtaggg ctgatttgac cacgttagcc gccttcgggg gtagtactat cagtttgcca
     4201 ttgtttccag gggttgccgc cgccgataat ttagccagcg gtttcattct gaatcgtcat
     4261 atagaatatg agagtctttg ctttagtttg tttcctggtg gccggctctc tttgtgccga
     4321 gactctcctt ttgaaaagcg gggccagagt agagggaacg atcatttcac agaatcggac
     4381 cacggttacc attcgcactg ccggcggaga tcaaacattt cgtaaagacg ctatccgccg
     4441 gattcaatat ggtcagacgg ccgcggagag agaagcggag aaggcagctg cagagaaacg
     4501 agccgctgaa aaagcagaag cagatcgaat cgcagcagaa aaagcagcgg cagaaaaatc
     4561 agaagcaaag gtcaccactc cgctggagaa agtggaagag aaaccggaag caaatacgaa
     4621 aact
//
LOCUS       DAOQJL010000302         4617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11088_length_4617_cov_4.488821, whole genome shotgun sequence.
ACCESSION   DAOQJL010000302 DAOQJL010000000
VERSION     DAOQJL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11088_length_4617_cov_4.488821"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLG78_12760"
     CDS             <1..256
                     /locus_tag="PLG78_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24037.1"
                     /translation="FKSGDLVWKSNEPAGLAYAFSDESFLAFANGTRTEVIDANGNRI
                     ARIPSPFGSGEPYQILPGPRKQQFLLQSYTGLVVFGFVSE"
     gene            complement(317..1252)
                     /locus_tag="PLG78_12765"
     CDS             complement(317..1252)
                     /locus_tag="PLG78_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24038.1"
                     /translation="MKSKLITALAVICAAGVLTAEPKVIDRIAEAKDYPDLQNTEKEQ
                     DNNIQDAYKRLTAYAPLVALAAKDRANSFKERAEPLRFEHTFRNVKYTPRNTYVRYVK
                     ESDKAPGDVMLVGLGDLKTIEDLIAQKTAQANAAGMPNGEQIAPFKFGNRDGVELTQF
                     QFIIREDADKIRAIGSRRKSVILFYKPAAAATPDTIGAQDLDLVVTRIVEDDYDAGVK
                     DVEVIIDPSPSTPGTNDVITLHRYNYKVTTCAILGTMSNTPNYPHRAAFKQKFYARLM
                     DHFDTLYRMVDTYAKRDGNDVNEKTIDRMNHSMDY"
     gene            complement(1353..1706)
                     /gene="secG"
                     /locus_tag="PLG78_12770"
     CDS             complement(1353..1706)
                     /gene="secG"
                     /locus_tag="PLG78_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNE24039.1"
                     /translation="MAIIQTVILVVFFICCAGLIFLILIQSGKGGSMGIMGGGGSNTA
                     FGSSTVDVVEKAAFWFAIAFFVLAILAAIAFADSGPRVPEKDAQTTEVPATQGTPANQ
                     ANPAPANPAPAPAQP"
     gene            complement(1738..2865)
                     /gene="pgk"
                     /locus_tag="PLG78_12775"
     CDS             complement(1738..2865)
                     /gene="pgk"
                     /locus_tag="PLG78_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNE24040.1"
                     /translation="MQRIETLSVSNQKVFVRADLSGLGDPPSGKALSRILPVVEILLG
                     KQAGVVLAGHDSNQNTFAPLADAIGKSLGRPVHLVEPGDMKQRWSTLKAGQVILLENL
                     SKYDGETKGENAFAEQIGGGADLYVSDCFAALALPYASITKLTKNKTIAAGPGLHSIL
                     LSLQEINSQKNKPLVSFLGGNDFAKKVDFMPRMLRLSQTALIAGSIAYTFLKSRLVPV
                     GNSIVDNGLEVTAFQLQEKAELEQTQFILPVDHMIGERFSRDAKAKASRDLPDRWMGL
                     DIGPKTIASFEKALKGAGSILWYGTAGAVELPAFQTGTKSLMKFASKLKAPITFLGED
                     TVRMADEFTFASATMIPGSESAIQVLLGKNPPGLALLQKEI"
     gene            2951..3511
                     /locus_tag="PLG78_12780"
     CDS             2951..3511
                     /locus_tag="PLG78_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNE24041.1"
                     /translation="MKKPILLKRYANRRLYDAETSRTLTLEDVAARIRSGHEIKVVEN
                     ATGEDITAYILGQTFLKVSLDRDHPEFSSFLLTALIREISGNVSHFFHRLVRGGIGFD
                     NLTRERMESIAARLVEKGELQIAERSEYVDRLMNQLHESRLDQIEKTTEAGKDRLREK
                     LNRDDRIEELSVKLQELSGFLQDLRK"
     gene            3583..>4617
                     /locus_tag="PLG78_12785"
     CDS             3583..>4617
                     /locus_tag="PLG78_12785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE24042.1"
                     /translation="MRQISFSIALLIVAGPVFAGSEDDIIQFNQARSEEALRNLDAGS
                     FDQKYLASLYMGGTRNPRFVRPLGRELLIGLQDPRGPRDPESTEIRIACGAQLNQICP
                     PTATQDPYIKASIAWALGEIGHKQAVPFLKEAMALVTQRIEVDVKRTSDLRAKLPGGE
                     YKNSVVLDRNTAGPAHLRKGYVYPFSPDIHWSEADDFKALGAVDEKDEGHRALMHSYN
                     FVNVAAMILQALGRIRDPSSLETIQAYLDHPIISIRGPAILAAGRQGRSALPVLLAQV
                     DKEKDKRMQARLARGILLIDKSQNAQYKVLITLLKDRDTEVRTEAVFALNELSMGEAV
                     DDLREALRIEE"
BASE COUNT         1125 a         1117 c         1212 g         1163 t
ORIGIN      
        1 tttcaagtca ggggatctgg tctggaaaag caatgaacct gccggacttg cgtacgcgtt
       61 ttccgatgaa tcttttctgg cctttgcaaa tggaacgcga acagaagtga tcgatgcaaa
      121 tggaaataga atcgctcgca ttccttctcc gtttggatcc ggggagcctt accagatatt
      181 gcctgggcct cgcaagcagc agtttcttct tcagtcttac actggactgg ttgtattcgg
      241 attcgtatct gaataagcgt acgtgcaaaa aaaaacccgc cgtgaagcgg gtttcttcct
      301 gttgagaagg ggattctcag tagtccatgc tgtggttcat gcgatcgatt gttttttcgt
      361 tcacatcgtt tccgtcacgc tttgcatatg tgtctaccat gcggtagagc gtatcaaagt
      421 gatccatcag tcgagcgtag aacttttgct tgaacgccgc gcgatgaggg tagttgggag
      481 tgttggacat ggttcccaga atcgcgcagg tagttacctt gtaattgtag cgatgcagag
      541 tgatgacgtc attggttccc ggagtagaag gggatggatc aatgatcact tccacatctt
      601 ttacacctgc gtcatagtca tcttccacga tgcgagttac tacaagatcc agatcctgcg
      661 caccaatggt gtccggtgta gcagcagctg ctggcttata gaaaagaatc acactcttgc
      721 gacgtgatcc gatggcgcgg atcttgtctg cgtcttcgcg gatgatgaat tggaattgag
      781 tcaattcaac gccgtcacga ttgccaaact tgaagggagc aatctgttcg ccatttggca
      841 ttcccgcagc atttgcctgg gccgtcttct gtgcgataag atcctcaatt gtcttgagat
      901 ctccgagacc aacaagcatt acatcgccag gagctttgtc gctttctttg acataacgca
      961 catacgtatt gcgaggggta tacttgacgt tccggaaagt atgttcaaaa cgaagaggct
     1021 cagccctttc cttgaaagag ttcgcacggt cttttgcggc taaagcaacg agtggagcat
     1081 atgctgtcag tcgcttgtat gcgtcttgaa tgttgttatc ctgctctttc tccgtatttt
     1141 gcagatctgg ataatcttta gcttccgcta tgcgatcgat gactttgggt tccgctgtga
     1201 gaacacctgc agcgcagatc accgcgagag ccgtaatgag cttgctcttc ataaaaaatc
     1261 ccttccttcc catgccaagg ctatatctag atcattatag gagggtgact gcctgaaatt
     1321 tagccgttcg aaaaaaaaaa tgaatttttt gcttaaggtt gagcgggcgc cggagcagga
     1381 tttgccgggg cagggtttgc ctgattggcc ggagtcccct gcgtggctgg gacttccgta
     1441 gtctgagcat ccttttcggg aacgcgagga ccagaatcgg caaatgcaat ggctgccaga
     1501 attgcgagga caaagaacgc gatggcaaac cagaaagctg ccttttctac cacgtctacg
     1561 gtagaagagc caaacgcagt gttcgatccc cctccgccca tgatccccat ggatccgcct
     1621 tttccggatt gaatcaggat caggaagatg agaccagcgc aacaaatgaa gaaaacaaca
     1681 agtatgacag tctgaatgat ggccatatat atagtaaatc tgcacctgag cgctatgtca
     1741 aatctctttt tgcagaaggg ccaatccggg cggattcttt cccaaaagta cctgaatggc
     1801 ggactcgcta ccggggatca tcgtggccga ggcgaaagta aactcgtctg ccatgcgaac
     1861 tgtgtcctcc ccgaggaatg tgatcggtgc tttgagtttg gaggcgaatt tcatcaggga
     1921 ctttgtcccc gtttgaaatg ctggaagctc gactgctccg gcagttccat accatagaat
     1981 ggacccggcg cccttcagtg ccttttcaaa ggatgcaatg gtcttaggcc cgatgtccag
     2041 gcccatccag cgatccggta gatcgcgcga agctttcgct ttcgcgtctc tggagaagcg
     2101 ttcgccaatc atgtgatcca ccgggagaat gaactgtgtt tgctcgagct ctgctttctc
     2161 ctgcagctgg aatgcggtga cttccagtcc attgtccacg atggaatttc caacgggcac
     2221 aagcctcgat ttcaggaatg tataggcaat gctgcctgcg atgagtgctg tctgcgaaag
     2281 tctgagcatt cgcggcatga agtctacttt ctttgcgaag tcgtttccac ctagaaaaga
     2341 cacgagaggt ttgttcttct gcgaattgat ttcctgcaga ctgagcagga tagagtggag
     2401 cccggggcct gcggcgatcg tcttgttttt tgtgagtttg gttatggaag cgtagggaag
     2461 tgccagggct gcgaagcaat cgcttacgta tagatccgcc cctccgccaa tttgctccgc
     2521 gaatgcgttt tcccctttcg tctctccgtc gtatttgctg aggttttcga gcaggatcac
     2581 ctggcccgcc ttgagagtgg accatctttg cttcatatcg cctggttcta ccaggtgaac
     2641 gggccgaccg agtgactttc caatcgcatc tgccagggga gcgaatgtgt tttgatttga
     2701 atcgtggcct gctagaacga cgcccgcctg tttgcccagg agtatttcca cgactggcag
     2761 tatgcgggac agtgctttcc ccgatggtgg atcgcccagg ccggaaagat ccgcgcgcac
     2821 gaataccttc tggttagaca ccgaaagagt ttcaattctc tgcatcgcgc gatgactcca
     2881 cgcggggtcc cactgtcatc tcaaaagtag cttgacgatc caaacgttca tggtgcactg
     2941 caaaaagaca gtgaagaaac ccattttatt gaaaaggtat gccaaccggc gcctctacga
     3001 cgctgagacc agtcgaacgc tcactctcga agatgtggca gcgcggattc gcagcgggca
     3061 tgaaatcaaa gtagtcgaga atgcaacggg ggaagacatc actgcataca ttctggggca
     3121 gacttttcta aaagtaagcc tggaccggga tcatcctgaa ttcagttctt ttctactcac
     3181 tgctctgatc cgtgaaatca gcggcaacgt ttcccacttc tttcacaggc tcgtgcgcgg
     3241 tggaattggt tttgataatc tcactcgcga acgtatggag agcattgctg ctcgcctggt
     3301 ggagaaaggg gaacttcaga ttgcagagag aagtgagtat gtagatagat tgatgaatca
     3361 gctgcacgag tcgcgtctgg atcaaattga aaagacaacc gaggctggca aagacaggct
     3421 tcgtgagaaa ttgaacagag atgatcgcat agaagagctt tccgtgaagc tccaggaatt
     3481 gagcggattt ctccaggatt tgcgaaaata gattacattt tttgaagcgc actttaagaa
     3541 tcgtctgacc gactccgaca gacaaactac aaaggaatgc ccatgcgcca aatcagcttt
     3601 tctatcgcac ttttgatcgt ggccggtcca gtcttcgcgg gatcggaaga tgacatcatt
     3661 caattcaatc aagcacgcag cgaggaagca cttcgcaatc tcgacgccgg aagctttgat
     3721 caaaaatatt tagcttctct ttatatggga ggaactcgta atcccagatt cgtgcgacct
     3781 ctcggtcgtg agcttctgat tggtcttcaa gatccacgtg gtccgcggga tccggagagc
     3841 actgaaatca gaatcgcatg cggagcgcag ctcaatcaaa tatgtcctcc taccgcaact
     3901 caggatccgt acatcaaagc gtcgattgca tgggcgctgg gcgaaatcgg acataagcag
     3961 gctgttccat ttttgaaaga agcaatggcg ctggtcaccc aaagaattga agtggatgtg
     4021 aagcgcacct ctgatctcag agcgaaactt ccaggtggcg agtataagaa ttctgtagtg
     4081 ctcgatcgaa acactgctgg cccggctcat cttcgcaagg gatatgtgta tcctttcagc
     4141 ccggacattc actggagtga ggcagacgac ttcaaagctc tgggcgccgt ggatgaaaag
     4201 gacgaagggc atcgcgcgct catgcacagc tataattttg taaacgtggc cgccatgatc
     4261 ctccaggcgc tcggaagaat tcgtgatcca agttctcttg aaacaattca agcatattta
     4321 gatcatccca ttatttctat tcgtgggccc gctatccttg ccgcaggtcg tcaggggagg
     4381 tccgcactgc cggtacttct ggcgcaagta gataaggaaa aagacaagcg gatgcaggca
     4441 agactggcgc gcggcattct gctgatagac aaatcgcaga atgcgcaata caaagtcctg
     4501 attactttgc tcaaagacag agacacagaa gtgcgcacgg aagcagtttt cgcactcaat
     4561 gagctttcca tgggcgaagc cgtagatgat ttgcgagagg ctcttcggat cgaagaa
//
LOCUS       DAOQJL010000303         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11219_length_4582_cov_4.538767, whole genome shotgun sequence.
ACCESSION   DAOQJL010000303 DAOQJL010000000
VERSION     DAOQJL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11219_length_4582_cov_4.538767"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLG78_12790"
     CDS             <1..1060
                     /locus_tag="PLG78_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE24043.1"
                     /translation="VSLARMSKIDYGSGLLHVGAGAITANIHRRAEQEGMMFPVDFAS
                     SGSSFIGGNIATNAGGIHVIRYGMIRNWIVSLRVVTGAGQILDLGANVQKDNTGPDLK
                     HIFIGSEGIFGIITEAVLRLTRKPHDTTLGMTGAASLEEVLGRLQEAKATTDLLAFEC
                     FDRRSALEVMAHLDLPEPMAISPWYAILEWEGEAEFPFDLAVAISSEQKKQFWKYREG
                     ISESLSRHRIYKNDVSIPGGDMPSYVEKIRNLAESLELDLAVFGHAGDENLHINLICR
                     SSMSADEFNDRAAQFTVQSYELIRSYKGSISAEHGIGLLKREDFARYGDPARIELLRS
                     IKKLLDPSGIMNPGKVLI"
     gene            1138..1350
                     /locus_tag="PLG78_12795"
     CDS             1138..1350
                     /locus_tag="PLG78_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24044.1"
                     /translation="MPSDRKLVACMQEHEMKTVLKHFKKGATEANVEAIQKACKAFKS
                     LDSYRPHNRDNFYKYIADKNVLKSLA"
     gene            1372..2742
                     /locus_tag="PLG78_12800"
     CDS             1372..2742
                     /locus_tag="PLG78_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNE24045.1"
                     /translation="MKPITLAASLAAFALFFACKQEPVPTTPARDFVYLLQVDGAITP
                     ASLEQLETALDQSEQDGARALILMLDTPGGLVTSMDGMIRRILASKIPVLTFIGPAGA
                     SCGSAGVYILYASHVAAMAPGTNIGSATPVMIGSGGETAKPSSSDIPKEAGADDQINL
                     KRKQIHHAIGQIRSLAEYHGRNADFGEKTITEAANVTSTTALQIHAIDLIVSTPEELL
                     ASVQGKRVRMLTGTQELLLNHAEIRKLDQGVRTRFLTFLAHPAVASILMMIGVLGILG
                     EIQYPGSIFPGVIGALCLLLGLYAMQTLPVNYAGAGLILLGVILMILEIKVVSYGMLA
                     VAGVISFALGGVMLVRSGGEVEKATIALIAGSGIAIGSVMAALIYLARKSQNADVQSG
                     AEQMLRSIGMAETVISPSGGRVRVHSELWNARTEGSEIPEGAKVRILERDGTFLRVER
                     VADKAP"
     gene            2771..4012
                     /locus_tag="PLG78_12805"
     CDS             2771..4012
                     /locus_tag="PLG78_12805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE24046.1"
                     /translation="MDKRRFEHRAFAALKVATLATVILCNPLSRSLSAEPSCAIGMGT
                     VCVDAVQRGETVTFTAQNRETYEVTITINGNLKNMTSSMRLPYSRTLPGKFNGPILTW
                     KANPRGAWSWTYTFNWTPGSLEGRHDSSVVYDLPFHGAHKIVQGFHGSFSHTGDFEYA
                     VDWEMPVGTSVFAAREGVVIGARSNMNEGGPEERYRNSANFVLIRHSDGSIGSYDHFK
                     QGGVAVRVGDRVERGTLIGYSGNTGFSGGPHLHFFVFRAKDGFTRESFPIKFNTASGV
                     TVPQEGAVNSSPGSSSTEAVETAEQPRTTPGNVTGDLRVCGIVDNSGPRNCKGDYKTT
                     DKIFVYVPIKKPGAYKFRISFTKPFEKSQPVNTEITTEADWGYSYFWITPSEERSPSG
                     TWEATIYVNEHPEGSVRFNAK"
     gene            4037..>4582
                     /locus_tag="PLG78_12810"
     CDS             4037..>4582
                     /locus_tag="PLG78_12810"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNE24047.1"
                     /translation="MAKGATIFKAEVDIADMDREYYAHHSLTLACHPSETEERMMVRL
                     LAFCMFASDHISFGKGLSTQDEPDLYENDLTGAMNLWLDVGQPDERSIRRSCGKARSV
                     VVVGFGGRGVGIWWDQNKEQLDRLENLTILNLPLKSTQALSSIAARNMNLQCNIQDGT
                     MSIISGDDLIDVEPAVLHGTWP"
BASE COUNT         1235 a         1142 c         1184 g         1021 t
ORIGIN      
        1 agtctctctg gccagaatgt ctaagataga ctacgggtcc ggactgttgc acgtgggtgc
       61 aggtgccatc accgcaaaca tccatcgaag agcagaacag gaaggaatga tgtttccagt
      121 cgatttcgct tcgagcgggt cttctttcat tggaggaaac attgcaacga acgcaggcgg
      181 cattcatgta attcgctatg gaatgattcg caactggatc gtttctcttc gcgtggtgac
      241 gggtgccggc cagattcttg atctgggtgc aaacgttcaa aaggacaaca cagggccaga
      301 cctgaaacat atcttcattg gctcggaagg aatctttgga atcatcacag aagcagttct
      361 tcgccttacg cgaaagccac acgatacaac cctgggaatg accggtgccg cgagcctgga
      421 agaagtcctg gggcgattgc aggaggcgaa ggccaccaca gatctgctcg cattcgagtg
      481 ctttgataga agaagcgctc tggaagtgat ggctcacctg gatctgccgg aaccaatggc
      541 gatttcgccg tggtatgcca ttctagaatg ggagggagaa gcagagtttc ctttcgatct
      601 cgcggttgcg atttcctccg agcaaaagaa acaattctgg aagtacagag agggaatcag
      661 cgaaagcctg agtcgtcatc gcatctacaa aaacgatgtg tcgattccgg gtggagatat
      721 gccgtcctat gttgaaaaaa tacgcaacct ggcggaaagc ctggagctcg acctggcagt
      781 gtttggccat gccggagacg aaaacctcca tataaatctg atctgccgct cttccatgtc
      841 cgcagatgaa ttcaatgacc gagcggcgca gttcaccgta caaagctatg aactcatccg
      901 aagctacaaa ggtagcatca gtgcagaaca cgggatcggg ctactcaaga gagaagactt
      961 tgctcggtat ggagatccag ctagaattga actgttgcga tcgatcaaga aactactcga
     1021 tccttccggc atcatgaatc ctggaaaagt tttgatctga gagagttttt tttgcgaaag
     1081 cgttgactgt acttcgatgc actgtaattt cctgcttacc acccaacgga gaataccatg
     1141 ccaagcgatc gtaaactagt agcctgcatg caggaacatg aaatgaaaac tgttctcaag
     1201 catttcaaaa aaggcgcaac agaagcaaat gtggaagcca tccagaaagc ttgcaaagca
     1261 ttcaagtcac tggactccta ccgtccacac aaccgtgata acttctacaa gtacatcgcg
     1321 gacaaaaatg tgctcaagag cctggcgtaa gcttctcgac ggcgccatgc catgaagcca
     1381 atcactctgg ccgcatcact ggcggccttc gcactctttt ttgcatgcaa acaagaaccg
     1441 gttcccacaa ctccggcaag agacttcgtc tacctgttgc aggtggacgg agccattacg
     1501 cctgccagcc tggagcaact ggaaacagcg ctggatcaat cggaacagga cggagcgcgc
     1561 gctctcattc tgatgctcga tactccaggc gggcttgtta cttcgatgga tggaatgatc
     1621 cgtcgcatcc ttgcttctaa aattccggtt ctgaccttca ttgggccagc cggcgcctct
     1681 tgcggatctg caggagttta tattctctat gcttctcacg tggctgccat ggcaccagga
     1741 acgaacattg ggtcggccac tccggttatg attggatctg gcggagaaac ggccaaacct
     1801 tcctcgagcg atataccaaa ggaagcgggt gcggatgatc agatcaatct aaagcgaaag
     1861 caaattcatc atgccattgg acaaattcga tccctcgcag aatatcacgg aaggaatgca
     1921 gattttggag agaagacaat cacagaagcg gctaacgtta cttccacgac cgcgctccag
     1981 atccatgcca tagatctaat tgtgtctaca ccagaagaac tactggccag cgttcaagga
     2041 aagcgagtgc gaatgctcac tggaacccag gaactcctct tgaatcatgc tgaaatcaga
     2101 aaattggacc agggtgtgag aactcgcttc ctgacgtttc ttgcacaccc ggctgtagca
     2161 tccattctca tgatgatcgg agtgctcggg attcttgggg aaattcagta tccaggaagt
     2221 atttttcctg gagtcattgg agcgctctgt ctactgctgg gactgtatgc aatgcagact
     2281 ctaccggtaa actacgcggg agcagggttg attttgctgg gtgtcattct gatgattcta
     2341 gaaatcaaag tggtcagcta cggaatgctt gcggtggccg gagtcatatc ctttgcactc
     2401 ggtggtgtca tgctcgtgag atccggcggt gaagtagaaa aagcaaccat cgcactcatc
     2461 gcaggaagcg gaatcgccat tggctctgtg atggctgcac tcatctatct tgccaggaaa
     2521 tcacagaatg cagatgttca atcgggcgca gaacaaatgc ttcgatccat cggcatggct
     2581 gagactgtca tctccccttc cgggggaaga gtgcgagtgc acagcgaact ctggaacgcg
     2641 cgcacggagg gatctgaaat tcctgaaggt gcgaaagtac ggatcctgga aagagacggc
     2701 acgtttctgc gcgtggaacg ggtcgcagac aaagctccct gacttcaaaa aaatttaagg
     2761 agatcatcgc atggacaaaa gaagattcga gcatcgcgct ttcgcggccc tcaaagtggc
     2821 cacccttgca actgtcattc tgtgcaatcc tctctcgcgt tctctttctg cagaaccatc
     2881 ctgtgcaatt ggaatgggca ctgtatgtgt ggatgcagtg caaagaggag agacagtcac
     2941 tttcacggca cagaaccgcg aaacctacga ggtcaccatt acgatcaatg gaaatctcaa
     3001 gaacatgaca tcgtccatgc gtctccccta ctcacgcact ctgcctggaa aattcaatgg
     3061 acccatcctg acctggaaag caaaccctcg cggagcctgg tcctggacat acacgttcaa
     3121 ctggactcct ggatctctcg aaggcaggca tgattcttcc gtcgtatacg atcttccttt
     3181 tcatggtgcg cacaaaatcg tccaggggtt tcacggctct ttcagtcata caggggattt
     3241 cgaatacgca gtggactggg agatgcctgt aggcacttcc gtattcgcgg cgcgagaagg
     3301 agtggtgatc ggagcaagat ccaatatgaa cgaaggcgga ccagaggaaa gatatcgcaa
     3361 cagcgcgaac ttcgttttga ttcgacattc ggacggatcg atcggtagct atgatcactt
     3421 caagcagggc ggcgttgctg tgcgagtcgg cgacagagta gaacgcggaa ctctgattgg
     3481 atattcgggt aacactggtt tctcgggtgg cccccacctt cacttcttcg tattcagagc
     3541 aaaagacggg ttcacgagag aatcgtttcc tattaaattc aacacagcct ccggcgtcac
     3601 agtacctcaa gaaggtgcag tcaattcctc tcctggaagc agctctactg aagctgttga
     3661 aactgcagag caacctcgaa ccacaccggg aaatgtaaca ggagatcttc gcgtctgcgg
     3721 catagtagac aactcggggc cgagaaactg caaaggcgac tacaaaacta cagacaagat
     3781 ttttgtatat gttcccatca aaaagccggg cgcatacaaa ttccgcatat cgttcacaaa
     3841 gccatttgaa aagtcgcaac ctgtgaacac tgaaatcaca acagaagcag actggggata
     3901 ttcctatttc tggatcactc cgtcagaaga acgcagccca tccggaacgt gggaagcaac
     3961 catatacgta aatgagcatc cggaaggatc cgttcgattc aacgcaaaat gaaaaacctg
     4021 gcacctggag catagaatgg caaaaggtgc cacgatcttc aaagcagaag tggacatcgc
     4081 ggatatggac cgcgaatact acgcacacca ttctctgact ctcgcatgcc atccgtcaga
     4141 gacggaagag aggatgatgg tacgactgct tgcattttgc atgttcgcga gtgatcacat
     4201 ttcttttggc aaagggcttt ccactcagga tgaaccggat ctctacgaaa atgatctgac
     4261 gggcgcgatg aacctgtggc tcgatgttgg acagccagat gaaaggagca tccggagatc
     4321 gtgcgggaaa gcgcgctctg tcgtggtcgt gggttttgga ggacgcggcg tgggcatctg
     4381 gtgggatcag aacaaagaac aactggacag actggaaaat ctaactattc taaatcttcc
     4441 tctaaaatcc acgcaggcac tctcttcgat cgccgccaga aacatgaacc tgcagtgcaa
     4501 catccaggac ggaaccatgt ccatcatatc aggagatgat ctgatcgacg tagaacctgc
     4561 agtacttcac ggaacctggc ca
//
LOCUS       DAOQJL010000304         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11315_length_4556_cov_4.135970, whole genome shotgun sequence.
ACCESSION   DAOQJL010000304 DAOQJL010000000
VERSION     DAOQJL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11315_length_4556_cov_4.135970"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2617
                     /locus_tag="PLG78_12815"
     CDS             <1..2617
                     /locus_tag="PLG78_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE24048.1"
                     /translation="DLINDAKKTVDIAVYNLGRQTIIDALILAHERGVKVRMVGDVDE
                     GATDGYRQIEFYRQIPFSLGNATGIQHNKFAVVDSKYVFMGTGNYTDSDLMRNNNNFM
                     VMESDTLAREYTREFEQMFYGKFAGAKLAPTHPQRQHYVNLTPIELYFSPYDGDEAIK
                     RMVQLIDGAKVQIQFMIFAYTHDEISAAMIRAARRGVLVRGVHDLTFIRGTSEEAPRL
                     YSAGVWMSTGPFNREDGNENTSVIGVAAHGGKLHAKTLIIDGNIVCTGSFNWSTNAVE
                     NNDENLLVVQSPYVAAELQQQWEAVWAVSHPITNQLSHPSGEPASPGDVVISEVMWAG
                     SYDSSGVYTNDDDWIELRNMTNHSIDLSHWSLAWDTKDTYNYPIPDEFNWFEDSVNTR
                     HRSLGKLVIPANGYFLLKNANGAIATGVEDNKLSGTKNFSLDSGSFKLKLYDLTMRLI
                     DEAGDGDPPLSGKQDGINKRAFSMERFFYPTGHPLAGKPLPGSAIGSWYTSNGNNLTA
                     PGSLAGTGQLDSAFKFCDATNTQCTIGTPNTSGNGSASSSQAFGAINNVQNVPIDAYS
                     TSTTTAVIQMRWGMAAVPFVNCAAGCGSICSSTQLDPNDPSKILITTASPQTATCTLT
                     VAGGTADVSNPAGGGFAAGGTISFDGQYSNRAQVQIDRVHPTCASSEDRVIIRALSTG
                     TVHRLGIYYYDSFSPNPVLLYRMHDVPINAGEYIMVTLDRPNDTSEDLLLGSSPTYAI
                     NTAGGPIGCGGAFPAGLAEATTYEVYSSTAGISSTDGLIFVSYDLDALPMDVMCYSNR
                     DGDMASGLMTGGMRDVFRWGTSVYNLAAFPVDTANDYDVQNSCAFYTGSGSNNYVART
                     PTGTDTNRGSDFQQF"
     gene            2626..2955
                     /locus_tag="PLG78_12820"
     CDS             2626..2955
                     /locus_tag="PLG78_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24049.1"
                     /translation="MKKVLLVIFCIAFAITCNPKQPDDSSTLAILAILIATGVIKPAR
                     INTPGGTGDPGGGDGLHLLKGSCHLDQSCLVPRLMTDASVECSARGGSYSEEPCDCEA
                     TTCDWLN"
     gene            complement(2952..3143)
                     /gene="rpmF"
                     /locus_tag="PLG78_12825"
     CDS             complement(2952..3143)
                     /gene="rpmF"
                     /locus_tag="PLG78_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNE24050.1"
                     /translation="MALPKRKTSLQKKRQRRAHHAIGKPNLVACKSCGAFIMPHRVCP
                     SCGFYKNRVVLAPKGVQKA"
     gene            3231..4262
                     /locus_tag="PLG78_12830"
     CDS             3231..4262
                     /locus_tag="PLG78_12830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNE24051.1"
                     /translation="MSLSEQLKKIEVNARNGLAGAADLAALDAWHADYLSKKGSLTEI
                     LKGLKDLSPDEKRSTGALANTVREALESAFLARKREISQAEINERLKSETYDALRPRS
                     SHRGHLHPLTQITEELEDLFSFMGFTILDGPEVETDENNFGKLNFSDDHPARDMQDTI
                     WTAQEGVLLRTHTSSVQVRAMQILKPPFRILAPGRCFRYEETDASHENTFNQAEGMMI
                     GENLSVANLIHVMKTFLSSFFEREVTVRLRPGYFPFVEPGFELDIQCLVCGGPGCPVC
                     KHSGWVELLPCGMVHPNVLRQGGLNPEKWNGFAFGLGLSRLVMMRYGIEDIRHFMSGN
                     LRFLEQFPA"
     gene            4275..>4556
                     /locus_tag="PLG78_12835"
     CDS             4275..>4556
                     /locus_tag="PLG78_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNE24052.1"
                     /translation="MKLSYNWLNDYVDLKSIDIKDLINRLTLATCEVEEVTDAFPGIA
                     QLIIARIVSCEKHPQADRLQICQVDAGKFQGKIVCGAPNARPGILVPFAP"
BASE COUNT         1139 a         1149 c         1168 g         1100 t
ORIGIN      
        1 ggatttgatc aacgacgcca aaaaaaccgt tgatatagcc gtgtacaatc tgggtcggca
       61 gaccatcatt gatgcactca ttctcgcgca cgagcgcggc gtgaaagttc gcatggtggg
      121 agacgtagac gaaggagcca cagacggtta caggcaaatt gagttttaca gacagatccc
      181 cttttctctt ggaaacgcaa cgggcatcca gcacaacaag ttcgcggtcg tggacagcaa
      241 gtatgttttc atgggaacgg gaaactacac tgactccgat ttaatgcgaa acaacaacaa
      301 cttcatggtg atggaaagcg atactctggc gcgcgagtat acgcgcgaat tcgaacaaat
      361 gttctatggc aagttcgcgg gggcaaaact cgcgcccaca cacccgcaaa gacagcatta
      421 cgtgaacctc actcctatcg aactctattt ctctccttat gatggagatg aagcaatcaa
      481 acgcatggtg cagctgatcg acggcgcgaa agtgcagatt caattcatga tcttcgcata
      541 cactcacgat gagatttccg ccgccatgat ccgagcggcg aggcgcggag ttctggtgcg
      601 cggcgttcat gacctcacat tcattcgcgg aacttcagag gaagcgcccc gactctattc
      661 tgcgggagtg tggatgtcca ccgggccctt caaccgcgaa gatggaaatg agaacacgtc
      721 cgtcattggt gtggcagcgc acggcggcaa gcttcacgcc aaaaccctga tcattgacgg
      781 gaatatcgtc tgcacaggat cattcaactg gtccacaaat gccgtggaaa acaacgacga
      841 aaacctcctg gtagtgcagt ctccatacgt tgcagcagaa cttcagcagc aatgggaagc
      901 agtctgggct gtttctcatc caattacaaa tcaactttct catccaagcg gtgagcctgc
      961 ttcccctgga gatgttgtga tttcagaggt aatgtgggcg ggttcttacg actcaagcgg
     1021 cgtatacaca aacgatgatg actggataga gcttcgcaac atgaccaatc attcgattga
     1081 tctttcccac tggtctcttg cctgggatac aaaggataca tacaactatc caattcccga
     1141 tgaatttaac tggtttgaag acagcgtcaa tacccgccac agaagcctgg gaaagctcgt
     1201 aattccggct aacggttatt ttctcttgaa aaatgccaac ggtgcaattg caacgggggt
     1261 ggaagacaac aaactgtccg gcacaaagaa cttcagtctg gattcaggct ctttcaaact
     1321 caagctgtac gatctaacca tgcgcctgat tgatgaagcc ggagatggag acccgccatt
     1381 gtcaggcaaa caggatggaa tcaacaaacg cgcattcagc atggagcgtt tcttctatcc
     1441 aacagggcat ccactggcgg gtaaacctct gccgggatca gcgattggtt cctggtatac
     1501 aagcaatggg aacaatctga ccgcacctgg ttctctggct ggcacgggac aattggactc
     1561 tgctttcaaa ttttgcgacg caaccaatac tcagtgtacc attggcacac ccaatacttc
     1621 aggaaacggc agtgcttctt catcgcaggc gttcggtgcc atcaacaacg ttcaaaatgt
     1681 gccgatagac gcttactcta cttccaccac gactgcagtc atccagatga gatggggaat
     1741 ggctgccgtt ccattcgtga actgcgctgc aggttgcgga agtatatgct cgagcacgca
     1801 actcgatcca aatgatcctt ctaagatttt gattacaact gcttccccgc aaacggctac
     1861 atgcactctg actgtggcag gtggaacggc agacgtatcc aatcctgcgg gcggcggatt
     1921 tgcggcaggg ggcaccatca gttttgacgg acagtattcg aatcgtgctc aggttcaaat
     1981 agatcgagtg catccaacgt gcgcttcgag cgaagataga gtgatcattc gcgcactcag
     2041 tacgggcact gtacacagac ttggaattta ctattatgat tctttttcgc ccaatccagt
     2101 tctactgtat cgcatgcatg atgttcccat caatgcgggc gaatacatca tggttacatt
     2161 ggatcgtccc aatgatacct cggaagattt gttgcttggt tcaagtccaa cctacgcaat
     2221 caatactgcg ggcggtccta tcggatgtgg aggagcattt cctgcgggac tcgcggaggc
     2281 taccacatac gaagtgtatt catcgactgc cggcatttcc tctacagacg gcctgatctt
     2341 tgtttcctat gatctagatg cgcttcccat ggacgttatg tgctattcga atcgcgacgg
     2401 agatatggca tctggcctaa tgaccggtgg gatgcgtgat gtatttcggt ggggaacatc
     2461 tgtttacaat ctagctgcgt ttccagtgga cacagccaat gactacgatg tccagaatag
     2521 ctgcgcgttt tacacgggat ccggttcgaa taactacgtg gctcgcactc ctacgggaac
     2581 ggacacaaac agaggttcgg actttcagca gttttaggaa ttcatatgaa aaaagtactg
     2641 ctggttattt tttgtatcgc atttgcaatc acctgcaatc caaagcagcc ggatgattcg
     2701 agcactcttg ccatactcgc gattctcatt gccacaggtg tcatcaaacc ggctcgcatc
     2761 aacactccag gcgggacggg agatcctggc ggaggggacg gtcttcattt gttgaaagga
     2821 tcgtgtcatc tggatcagag ttgcctggtt ccgcgcttga tgacagatgc ttctgtagaa
     2881 tgtagcgcgc gtggcggatc ctacagcgag gagccgtgtg actgcgaagc aactacctgt
     2941 gattggttga attaagcttt ctggacgcct ttaggtgcaa gtaccacgcg gttcttgtaa
     3001 aaaccgcagg acgggcaaac gcggtgaggc atgatgaatg caccgcagct cttgcaggct
     3061 accaggtttg gcttaccgat tgcatggtgc gcccggcgct gacgcttttt ctggagagat
     3121 gtcttccgct ttggtaaggc catgaaccag acttgcaaga gagccctgtg cgggcaacct
     3181 ttttcgcgag catgcttgac agcgtgccgc ccgcgcaaat cccaatcgta atgtcccttt
     3241 cagaacaact caagaaaata gaagtaaacg ctcgaaatgg tctggcgggt gccgcagatc
     3301 tggcagccct ggacgcctgg catgcggatt atctcagtaa aaaaggcagt ctcacagaga
     3361 ttctaaaggg actcaaggat ctttcccctg acgaaaagcg aagcacgggc gctctggcaa
     3421 atacagtccg cgaggcgctt gagtctgcgt ttttggcgcg caaacgcgaa atttctcagg
     3481 cagaaatcaa tgagcgcctg aaaagtgaaa catacgatgc gctcagaccc agatcttctc
     3541 atcgcggaca cctccatcct ctcacccaga ttacagaaga actcgaagac ctgttttctt
     3601 tcatgggctt taccatcctc gatgggcccg aagtagaaac ggatgaaaac aactttggca
     3661 aattgaattt ctcagacgat catccggcgc gcgatatgca ggatacgatc tggactgccc
     3721 aggagggtgt acttcttcgc actcacacct cttccgtaca ggtgagggcc atgcagattc
     3781 taaagccgcc gtttcgcatt ctggcgccag ggcgttgctt cagatacgaa gagacagacg
     3841 cttcgcatga aaacacattc aatcaggcag aaggcatgat gatcggtgaa aacctttcgg
     3901 ttgcgaattt gattcatgtg atgaaaacgt ttctgtcttc cttctttgag cgcgaagtca
     3961 cagtgcgctt gcgtcctgga tactttccgt ttgtggaacc gggcttcgag ctggacattc
     4021 agtgcctggt ctgtggcgga cctggttgtc ctgtgtgcaa acacagcggc tgggtggagc
     4081 ttcttccatg tggtatggtg catccaaacg ttcttcgtca gggtgggctc aatcctgaga
     4141 aatggaacgg ttttgctttt ggtctggggc tttcgcggct tgtgatgatg cgatacggaa
     4201 tcgaggatat tcgtcacttc atgtccggca atctacgctt cctggaacaa tttccagcct
     4261 aatcgagtaa cacaatgaaa ctttcatata actggctcaa tgactacgtc gatcttaaat
     4321 ccattgatat caaggatctg atcaatcgcc taacacttgc tacatgcgaa gtggaagagg
     4381 tgacggatgc attccctgga atcgctcagc tcatcatcgc tcgcattgta agttgcgaga
     4441 agcacccgca ggcagaccgt cttcagatct gccaggttga tgccggaaaa tttcagggca
     4501 aaattgtatg cggagcgccc aacgcccgtc cgggaattct ggttccgttt gctcct
//
LOCUS       DAOQJL010000305         4553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11326_length_4553_cov_4.018453, whole genome shotgun sequence.
ACCESSION   DAOQJL010000305 DAOQJL010000000
VERSION     DAOQJL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11326_length_4553_cov_4.018453"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..380
                     /locus_tag="PLG78_12840"
     CDS             153..380
                     /locus_tag="PLG78_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24053.1"
                     /translation="MASKILKVVSPMPAASHSAGSSQKEKQAIAKVKVSVDKAKIARK
                     IARSQVPVPAPRAHASKTKYTRKVKHRKQPE"
     gene            377..931
                     /locus_tag="PLG78_12845"
     CDS             377..931
                     /locus_tag="PLG78_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNE24054.1"
                     /translation="MKRIPIALRVLIAWFLVHTSFILYDGFSDSVEPGHTAIVFGNKV
                     EETGLPSERLERRLIRALDLYNQGTANHLFVSGGVGKEGFDEALIMKQYLVAHGVPEE
                     AITQDSEGVDTRATARNFAVAGGGPGAIVVTDYFHISRAKLALRNVGLPTRFSAHAPF
                     RFHIKELYYVPREFFGYYFYLIRR"
     gene            complement(955..1293)
                     /locus_tag="PLG78_12850"
     CDS             complement(955..1293)
                     /locus_tag="PLG78_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE24055.1"
                     /translation="MASYAINNNYKIKVLEISGRFTIENRDEFQAFIKENVDMSAPGT
                     CIDATDLGYIDSSGIGELLRLKMSAAKTNHVIYVFGLSDAILKMFKMSQLNHVFVIMT
                     KEEFESQFKA"
     gene            1371..1793
                     /locus_tag="PLG78_12855"
     CDS             1371..1793
                     /locus_tag="PLG78_12855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24056.1"
                     /translation="MAEPETRNRIRLQFPSQPRYVATIRDTIYRFCLQHGFARAAAFD
                     VKVIAGEALTNIIQHAYGGGADHPIFIEMLFFKEYGEFRFKDLGKQVPITNQMKKDLT
                     DYREGGLGLYLIDQLSDFHVYDQSEKVGTTLIVKKKMA"
     gene            1790..2608
                     /locus_tag="PLG78_12860"
     CDS             1790..2608
                     /locus_tag="PLG78_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24057.1"
                     /translation="MTWTLYDERLRNPWLALAIDEAVCHFFAGKEFDSLIRVWRNPSA
                     ICFGRTCDASLNAHGESLLNLDPGFSRSHFENRTVLLRRASGGGTVLHGPGNWNYSII
                     VSLDKFPEVFPVKQSYERFLGLAISALAQQGIEAHAAGQSDLVTPVQGELRKFSGNAQ
                     FRKRGICVHHGTLLEDSSLISGISRALPHPPREPDYRAGRSHDRFLGSLPAGFSIQQF
                     ARDLSSATASWTASEVRPLSADVRKKIFQLAGKLAKKIYTNREWILQGKAPDFH"
     gene            2621..3877
                     /gene="glyA"
                     /locus_tag="PLG78_12865"
     CDS             2621..3877
                     /gene="glyA"
                     /locus_tag="PLG78_12865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNE24058.1"
                     /translation="MLGQFLKQSDPDVYGALVKEDERQENSLEMIASENFVSRSVLEA
                     YSSTLTNKYAEGYPGKRYYNGCEHADTIENIAIERAKQMFGAGFANVQPHSGAQANMA
                     VFLGFMNPGETFLGMDLAHGGHLTHGSKVNFSGRLYNVASYGVDPNTHRIDMDNVRKI
                     ARETKPKMIIAGFSAYPRVLDFAKFREIADEVGAVLMADIAHIAGLVVAGEHPTPVGQ
                     AHITTTTTHKTLRGPRGGLILTTSEDHAKTINSRVFPGVQGGPLMHVIAAKAVAFGEA
                     LKPEFKDYIKRVKENAKVLAETFMSRGFTVVSGGTDNHLILLNVGARGLTGAVAADAL
                     HEAGITANKNGIPFDPNPPAVTSGVRFGSPALTTRGFGKDEFVRVGNLICDLLSDAQN
                     PANIQKVNGGVLELARKFPMAGFRLG"
     gene            complement(3928..>4553)
                     /locus_tag="PLG78_12870"
     CDS             complement(3928..>4553)
                     /locus_tag="PLG78_12870"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE24059.1"
                     /translation="FLGLCMMTAVVWLISILWGMLDRSSFAWFGAAMVAAFAGAMAYG
                     QSSQKTENMKRSLRAAALILIPLSFLIAGFRSTAEIRKQADVVIETGVWLPYSKTSLE
                     QARHAGPVFVDFTADWCLSCKVNEKAALGKSDVMLLFKKKNVILFRADWTVESPEITE
                     ALAKFDRNSVPLYVLYPGKEQEPRILPQILTPAIIQEELKTLPDAID"
BASE COUNT         1135 a         1129 c         1166 g         1123 t
ORIGIN      
        1 aatgcatgcg gcgctttccg ggcagaattg tgtttggaac tgtacttgcc gaagatctaa
       61 aaggtcgggt cctcgtatgc aattctgtgc gaggcatcat gcgcgctcga atatttgact
      121 cgacgaaatg attctgtgga cgaagtagaa ctatggccag caagattctg aaagtagtgt
      181 cgcccatgcc ggccgcatcg cattctgccg gatcttccca gaaggagaag caggccatcg
      241 cgaaggtcaa agtgagcgtg gataaggcaa agatcgcgcg caaaattgca agatcacagg
      301 tgcctgttcc tgctcccaga gcgcacgcct cgaaaacaaa atacactcgc aaagtaaaac
      361 ataggaagca gcctgaatga aaagaattcc catcgcgctg agagttctaa tcgcctggtt
      421 tctggttcat acttcattca ttctttatga tggtttctcg gactcggtag aacccggtca
      481 tacagctatt gtcttcggaa acaaagtgga agaaaccgga ctcccttcag agaggctgga
      541 gcgcagactc attcgcgctc tcgatctcta caatcaagga acggccaatc atcttttcgt
      601 gagcggtgga gtgggcaaag aaggtttcga tgaagcactg atcatgaagc agtatctggt
      661 cgctcacgga gttccggaag aagccatcac gcaagatagc gaaggtgtgg atactcgagc
      721 cactgcacga aacttcgccg tcgccggtgg tggaccagga gccattgtgg ttacggacta
      781 ctttcacatt tcgagggcaa agctggcgtt aagaaatgtg ggcctgccca cgaggttctc
      841 cgcgcatgca ccgtttcgat ttcacatcaa ggaactctac tacgtgccgc gcgaattctt
      901 tggctattac ttctatttaa taaggcgctg aggcggcggg gcgcggacct ggcctcaagc
      961 tttgaactgg ctttcgaatt cttcttttgt catgatcacg aacacgtgat tgagctgaga
     1021 catcttgaac atcttgagga ttgcatctga aagtccaaac acatagatca cgtgatttgt
     1081 ctttgctgca gacattttga gtctgagtag ttctccaatg cccgatgagt caatgtagcc
     1141 aagatccgtg gcgtcgatgc aggtgccagg agcgctcatg tccacgttct ctttgatgaa
     1201 cgcctggaat tcgtccctgt tttctattgt gaatctgcct gaaatctcga ggactttgat
     1261 cttgtagttg ttattgatcg cgtaagatgc cacgccgaag cgtccatttc aaacaggccc
     1321 tgtcaaacac ttttatcttg ccctggcggg ccgggaggac agactatccc atggccgagc
     1381 cggagactcg aaatcgcatt cgcctgcaat tcccttctca acccaggtac gtcgcgacca
     1441 tccgggacac catctatcgc ttttgcttac agcacggatt cgcgcgtgcc gccgctttcg
     1501 atgtcaaagt cattgccggg gaagcgctca ccaatattat acagcatgcg tatggcggcg
     1561 gcgcggacca tccgattttc atcgagatgc tcttcttcaa agaatatggc gaattcagat
     1621 tcaaggatct tggcaaacag gttccgatta ccaaccagat gaagaaagat ctcaccgatt
     1681 accgcgaagg tggtctgggg ctatatctaa ttgatcagct ttccgacttt catgtttacg
     1741 atcagtcgga gaaagttgga accacgctca ttgttaagaa aaagatggca tgacctggac
     1801 gttatacgac gagcgtctgc gaaatccctg gttggcactg gccattgatg aagcagtctg
     1861 ccatttcttt gctggaaagg aattcgatag cctgattcgc gtttggagaa atccatctgc
     1921 gatatgtttt gggagaacgt gcgatgcttc tctcaacgcg cacggggaat ctctgctcaa
     1981 ccttgacccg ggctttagtc gttctcattt tgagaatagg acagtactgc ttcgtcgggc
     2041 ttccggaggt gggactgtgc ttcatggacc aggcaactgg aactacagca tcattgtatc
     2101 tcttgataaa tttcctgaag tatttccagt aaagcaatcg tacgaacgct ttcttggtct
     2161 tgcgatttca gctctcgcgc agcagggaat tgaggctcat gcggcaggac agtcggatct
     2221 ggtcacgcct gttcagggtg aactcaggaa attcagcggc aatgctcaat ttcgaaaacg
     2281 aggcatctgc gtacatcacg gcacacttct ggaagactca tctttgattt ccgggatttc
     2341 gcgtgctctt cctcatccac ccagagagcc ggactacagg gcaggtcgtt ctcacgatcg
     2401 atttctgggc agcctgcctg ctggtttttc gattcagcaa tttgcgcgcg atttatccag
     2461 cgcaacggcc tcctggaccg cctcggaagt tcgccctctt tctgctgatg ttcgaaaaaa
     2521 gatctttcaa ttggcaggga agctcgcaaa aaaaatctat acaaatcggg agtggatcct
     2581 gcaaggaaaa gccccggact ttcattaaag cggtgataag atgctcggac aatttctgaa
     2641 acaatcggat ccagatgtat atggcgcact cgtcaaagaa gatgagcgtc aagaaaactc
     2701 acttgagatg atcgcgtcgg aaaacttcgt atcgcgaagt gtccttgaag catattcttc
     2761 tactctcacc aacaaatacg cggaaggtta tcctggaaag cgttactaca acggctgtga
     2821 gcacgcagac acaatcgaaa atattgccat cgaacgcgca aagcagatgt ttggtgccgg
     2881 tttcgcaaac gttcaaccac actcaggtgc tcaggcaaac atggctgtgt ttcttggatt
     2941 catgaatccg ggagaaacct tcctgggtat ggatctggcc cacggtggcc acctgacgca
     3001 cggttccaaa gtgaatttct caggtcgtct ctacaatgtt gcatcctacg gtgtagatcc
     3061 aaacactcac agaatcgaca tggacaatgt gcgaaagatc gcacgcgaaa caaaaccgaa
     3121 gatgatcatt gccgggttct cagcgtatcc tcgcgttctg gatttcgcga agtttcgcga
     3181 gatcgcagat gaagtgggtg cagtgctcat ggcggacatt gctcacatcg caggtctggt
     3241 tgttgcggga gaacatccaa ctccagtggg ccaggctcat atcactacca ctaccacgca
     3301 caagactctt cgcggtccac gcggcggtct gattctgacc accagcgaag accatgcgaa
     3361 gactatcaat tctcgcgtat ttccgggagt tcagggtggt ccactcatgc atgtgattgc
     3421 ggccaaggca gttgcatttg gagaggcgct gaagcccgag ttcaaggatt acatcaagcg
     3481 tgtgaaagaa aatgcgaaag tgctcgcaga aacttttatg agccgggggt tcaccgtagt
     3541 ttcgggcgga acggacaatc acctaatcct cttgaatgtg ggtgcgcgtg gcctcacagg
     3601 tgcagttgcg gcagacgcac ttcacgaagc tggaatcacc gcgaacaaga atggaattcc
     3661 atttgatcca aatcctccag ctgtcacaag tggtgtgcga tttggaagtc cagctctgac
     3721 cactcgtggg tttggaaagg atgaattcgt gcgcgtagga aatctgattt gtgatttgct
     3781 cagcgacgct cagaacccgg ccaacatcca gaaagtgaac ggcggagttc tggaactggc
     3841 gcgtaaattc ccaatggctg gatttcgcct gggttgatgt tctgatgtct gatgtctgag
     3901 tcaggcgtca gctagcgtaa aggaacctca gtcgatcgcg tctggaagtg ttttcaattc
     3961 ttcctggatg atcgcaggag tcagaatctg tggaaggatt cgcggctcct gttctttgcc
     4021 cggataaaga acatacagag gcacgctgtt tctgtcgaac ttcgcaagtg cttctgtgat
     4081 ttccgggctt tccacggtcc aatctgcgcg aaatagaatt acattcttct ttttaaaaag
     4141 aagcatcaca tctgattttc caagcgctgc tttttcgttc accttgcagg aaagacacca
     4201 atctgcagtg aaatccacaa acacaggtcc agcatgccgt gcctgctcca gagacgtctt
     4261 gctatagggg agccatactc ctgtttcgat tacaacgtct gcctgcttgc gtatttctgc
     4321 cgtggaccga aacccagcga tcaagaaaga aagaggaatc agaatcaaag cggccgcgcg
     4381 aagagatcgt ttcatgtttt ctgttttttg cgaagactgt ccgtatgcca tagctccagc
     4441 aaacgcggca accatggctg cgccaaacca ggcaaagctc gatcgatcca gcatccccca
     4501 tagaatggaa atgagccaga cgacagcagt catcatgcac agtcccagga att
//
LOCUS       DAOQJL010000306         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11352_length_4548_cov_5.267305, whole genome shotgun sequence.
ACCESSION   DAOQJL010000306 DAOQJL010000000
VERSION     DAOQJL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11352_length_4548_cov_5.267305"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="glnA"
                     /locus_tag="PLG78_12875"
     CDS             <1..1040
                     /gene="glnA"
                     /locus_tag="PLG78_12875"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE24060.1"
                     /translation="FGPEAEFFIFNDVRFDQNMNSGFYFIDSDEASWNMGRDEGPNLG
                     YKIRTKEGYFPLPPSDTFQNIRSEMLHEMEKAGIKTEKHHHEVASPGQAEINFVVDEG
                     IKMADKLQLYKYIVKNVARRHGYSATFMPKPIFGDNGSGMHTHQSLWKGGKNIFAGNK
                     YAGISDQCLNYIGGILSHGPALLAFTNPTTNSYKRLVPGFEAPVNLVYSARNRSASIR
                     IPIAVSASEKAKRIEFRTPDASCCPYIAFAAMIMAGLDGIQSKIDPGGPADFNLYDAT
                     PEQKAKLKSVPPNLTKVLDALEADHSWLTKGGVFDEDYISNYIEYKRAEVEKIVNQRP
                     HPYEFVLYYDC"
     gene            complement(1128..1538)
                     /locus_tag="PLG78_12880"
     CDS             complement(1128..1538)
                     /locus_tag="PLG78_12880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE24061.1"
                     /translation="MIITPIQTRWNDMDPFAHVNNANYVAYLEIGRVDYCVRRLGVTE
                     LYAVPFLLARIEVDMIRPIEFGAGVEVLTCVSKIGNKSWDFLAFIRDSKSKEDFARAR
                     TVQVAYDHRRKQSQVIPESLRSILHEDWKSYQEQ"
     gene            complement(1682..2806)
                     /locus_tag="PLG78_12885"
     CDS             complement(1682..2806)
                     /locus_tag="PLG78_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNE24062.1"
                     /translation="MKKVFVMGLVLCMSGLFTAANATVRKTANPVVFAHGLAGWDNLL
                     GYFYFGDGVGNFVGDACDEFLEVVCNGGLNSSQKAFAAGVQPFASSEVRGTQLADQIQ
                     SYLATVGATYVNIIGHSQGGIDARKAAVVLKQRKGFTVVKTMVSVSSPHRGSPVAKYI
                     LNLGPGVTSVVSALATFYGNTVYGAGNDPIAAAKQLVYNDYSSTDGVTTGMKAYNTAY
                     ASYSTAVSNRLASVITAQQGLDVNAALYILSKGFYDIDGDGASCAVASDDCDNDGALG
                     KGNGSKTDEDDDGLVGINSQQMGYRLAYSTCFLCDDYMTQDTGLGYLTDMNNPTSAQQ
                     SSHADVVHQDHLDVTGAGTTTLDRDEFYAAIIDYMAANGG"
     gene            3223..4272
                     /locus_tag="PLG78_12890"
     CDS             3223..4272
                     /locus_tag="PLG78_12890"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNE24063.1"
                     /translation="MQKRGSSSNPESRFIHTKREMEESDLDPDAVSSPSTELFADKSR
                     TVLSSNESPDVPFSQSVNPYRGCEHGCSYCYARPTHEYLDLSAGIDFESKIFVKYDAA
                     KLLKEEISKKSYKPDVICFSGVTDSYQPIERKLQITRACLSVLREFRNPVSIITKNHL
                     ITRDVDILGEMAKDNLSCVLVSVTTLDENIRSRMEPRASTAARRLETIRFLSDHGIPV
                     GVLVAPVIPGLTDHEIPAILKVVKEAGASFAGYVMLRLPHGLKELFLEWLGTNFPNQK
                     KKVTDRILDIRGGTLYDSSFGTRGRGEGAFAEQIQSLFRLSLKKFALNSVGPKLRTDL
                     FEPVQGLLFPDPNKS"
     gene            complement(4280..>4548)
                     /locus_tag="PLG78_12895"
     CDS             complement(4280..>4548)
                     /locus_tag="PLG78_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24064.1"
                     /translation="FAYDIWGDAVNVASRMESGGEPGRLNISGDTAEYVRTFFDLEPR
                     GEVAVKNRGTIPMYFVKGLRTGLQDSNGRPGPGFHDLYDRLLIA"
BASE COUNT         1193 a         1191 c         1082 g         1082 t
ORIGIN      
        1 ggttcgggcc agaagcagaa ttctttatct tcaacgatgt gcgctttgac cagaacatga
       61 actctggctt ctacttcatc gattcagacg aagcatcctg gaacatgggc cgcgatgaag
      121 gtccaaacct tggatacaaa atccgcacca aagaaggata cttccctctg cctccaagcg
      181 atacattcca gaacattcgc tctgaaatgc tgcacgaaat ggaaaaagcc ggaatcaaaa
      241 ccgagaagca ccaccatgaa gtagcaagtc ctggacaggc tgagatcaac ttcgtcgtag
      301 acgaaggaat caagatggca gacaaactgc aactttacaa atacattgtg aagaacgtag
      361 cgcgtcgtca tggatactcc gctactttca tgccaaagcc aatcttcggc gacaacggtt
      421 ctggcatgca cacacaccag tccctgtgga aaggcggaaa gaatatcttc gctggaaaca
      481 aatatgcggg aatttccgat cagtgcttga actacatcgg cggtattctc agccatggac
      541 cggctctgct cgctttcacc aaccctacta caaacagcta caagcgtctt gttccagggt
      601 ttgaagctcc tgtgaatctt gtatactctg caagaaaccg ttctgcgtcg attcgtattc
      661 cgatcgctgt cagtgcgagc gaaaaggcaa agagaattga attccgtact ccggacgcgt
      721 cttgctgccc atacattgca tttgctgcga tgatcatggc tggtctggac ggtatccaga
      781 gcaagatcga tccaggtgga ccggcagact tcaacctcta tgacgcaacg cccgaacaaa
      841 aagcgaaatt gaaatcagtt ccgcctaacc tcacaaaggt tctcgacgca ctggaagcgg
      901 accacagctg gctgaccaaa ggcggagtat tcgacgaaga ttacatcagc aactacatcg
      961 aatacaagcg cgcggaagtt gaaaaaatcg taaaccagag accacaccct tacgaatttg
     1021 ttctctacta cgactgctga tattcagctg ctttaccggg aagaagcgtc cgcgaaagcg
     1081 ggcgcttttt ttttgcagag aggggcttcc gcgcggactg ttattcctta ttgctcctgg
     1141 taggatttcc aatcttcgtg cagaatggaa cgaagggatt ccggaatcac ctgcgattgt
     1201 ttgcggcgat gatcatacgc tacctgcacg gttcttgccc ttgcaaaatc ttccttcgac
     1261 tttgaatccc gaatgaatgc cagaaaatcc caggacttgt ttccaatttt ggaaacgcac
     1321 gtcagcactt ctacgcctgc accaaattca atcggacgaa tcatatccac ttcgattcga
     1381 gcaagcagaa atggtacagc atacaattca gtcactccca gccgtcgcac acagtagtcc
     1441 acccggccga tttcgaggta agccacgtag ttcgcgttgt ttacgtgagc aaaaggatcc
     1501 atatcgttcc agcgagtctg gatcggagtg atgatcattc ttactcgatg tggacagcgt
     1561 gcgagcggtc aattgtttcc ctcagatgtt ggtaacgaat tgccgaggcg gcggaaggcg
     1621 cagacggagg caaaaaaaaa cccggccgcg aagccgggtc ctttcgattc agtgaataca
     1681 atcagccgcc gtttgcagcc atgtagtcaa tgatcgccgc gtagaattcg tcgcggtcta
     1741 gagtagttgt tccagcgccg gtcacatcca ggtgatcctg gtgaaccaca tctgcatgag
     1801 aactttgctg agcagaagta ggattgttca tgtccgtcag gtaaccaagg ccagtgtcct
     1861 gagtcatgta gtcatcacaa aggaagcaag tggagtacgc cagacgatat cccatctgct
     1921 gagagttgat gcctaccagt ccgtcatcat cttcgtcagt tttgcttccg tttccttttc
     1981 cgagagctcc gtcgttgtcg cagtcatcag aagcaactgc acaggatgca ccgtcgccgt
     2041 cgatatcata gaagcccttg ctcaaaatgt agagagcggc attcacgtcc aggccttgct
     2101 gggctgtgat cacagatgca agacgattgg atacagctgt ggagtagctc gcatacgctg
     2161 tattgtaagc tttcattcca gtcgttacgc catctgtgga gctatagtca ttgtaaacca
     2221 gttgcttggc tgctgcgatc ggatcgtttc ccgccccata cacagtgttt ccgtagaacg
     2281 tggccagtgc gctcactacg cttgtaacac ctggaccaag attcaggatg tactttgcaa
     2341 ccggtgagcc gcgatgagga gaagaaacgg aaaccattgt tttgacgaca gtgaaacctt
     2401 ttctttgttt cagaactaca gccgctttgc gcgcatcgat gccgccctgg gaatgaccaa
     2461 tgatgttcac ataggtagct cctactgttg caaggtagct ttgaatttga tccgcgagct
     2521 gagtgccacg aacttctgag ctagcaaatg gttgaacgcc tgcggcaaac gccttctggc
     2581 tggaattgag tccgccgttg caaaccactt ccaggaactc gtcgcatgca tcaccaacga
     2641 agttaccgac tccatcacca aagtagaaat acccgagcag gttgtcccat cctgccagac
     2701 cgtgtgcgaa aacgactgga tttgccgttt tgcgaaccgt tgcattcgct gctgtaaaca
     2761 agccactcat gcagagcacg agccccatca caaaaacctt tttcattttt ccccttcgcg
     2821 cgcacgaagg cgcacttttg atgactccac tcttgcatag agtgtaccag gtacgcgaaa
     2881 gcagtttttc gcacggcctc ttttgtattt cgtatctact gatttcgtgc atcgcaccag
     2941 tgatttcgct ttcgcacgcg caaggacgtt tcgcgtctaa aaatcctgta gcggtgcact
     3001 gcgatttttc gcatggatcg cgcgcgtgca aaagcgtttt cgcacatgaa ctgagagaag
     3061 catcatttct tatttcctgg attccaattt ataggaacgt tccagttttc aggaaatcac
     3121 taattctcgg gaacgaccga actaatccga agggactaga aacgtaaaca caatcgtcgt
     3181 ccaagggcat tgacaattgc gcagaagaat tgattctgat ccgtgcaaaa gcgtggaagt
     3241 tcttcgaatc cggaaagcag attcatccac acgaaacgcg aaatggagga atcagacctt
     3301 gatccagacg cagtctcttc tccatctacc gagcttttcg cagacaagag ccgcacggtt
     3361 ctaagctcaa acgagagccc ggacgttccc ttctcgcaaa gcgtaaatcc atacaggggt
     3421 tgcgaacacg gctgctccta ctgctacgcg cggcctactc atgagtatct ggacttatct
     3481 gctggaattg actttgaatc caaaatcttt gtaaaatacg acgccgcaaa actgctcaaa
     3541 gaggaaatct caaaaaagtc atacaaacca gatgtaatct gtttctcggg agttacggat
     3601 tcttaccaac caatcgaaag gaaactccaa atcacgcgag cctgcctttc tgttctgcgc
     3661 gagtttcgca accccgtatc catcattact aagaatcacc tgatcacgcg ggatgtagat
     3721 atcctgggcg aaatggctaa agacaacttg agctgtgtgc tggtatctgt gactactctc
     3781 gatgaaaaca tacgatccag aatggaaccc agggcatcca cagccgcgcg aaggctcgaa
     3841 accatccgct tcttgagcga tcatggaatc cccgtaggtg ttcttgtagc accggtcatc
     3901 ccgggcctga cggaccacga aattccagcc atactcaagg tagtgaaaga ggcaggcgcg
     3961 agtttcgcag gctacgtgat gctacgattg cctcatgggc tcaaggaact ttttcttgaa
     4021 tggctcggga cgaattttcc gaatcaaaaa aagaaagtaa cagacagaat cctggacatt
     4081 cgcggtggaa ctctctatga ttcgagcttt ggaacccgag gtcggggaga aggcgctttc
     4141 gcagaacaaa tccagagtct atttcgcctg tctctcaaga aattcgcttt gaattcggtc
     4201 gggccaaaac ttcgcacgga tctgtttgaa ccggtgcagg gacttctctt tcccgaccct
     4261 aataaatcat gaaagaacgt taggcgatca gtaacctgtc gtacagatcg tgaaagccgg
     4321 ggccgggtcg cccattggag tcttgcaaac ctgtgcgcag tcccttcaca aaatacattg
     4381 gaatggtacc gcgattcttg acagccactt cacctcttgg ttcaagatca aaaaacgtgc
     4441 gaacgtactc tgccgtgtcc cctgaaatat tcaatcgacc gggctcaccg cccgactcca
     4501 tgcgcgacgc cacattcacg gcgtctcccc agatgtcgta ggcgaatt
//
LOCUS       DAOQJL010000307         4547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11355_length_4547_cov_4.676536, whole genome shotgun sequence.
ACCESSION   DAOQJL010000307 DAOQJL010000000
VERSION     DAOQJL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11355_length_4547_cov_4.676536"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLG78_12900"
     CDS             complement(<1..600)
                     /locus_tag="PLG78_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HNE24065.1"
                     /translation="MSVLRFKDLDNSVSLERPEYKASLNDLQEKVRLLSHQAADENRS
                     IIVVFEGWDAAGKGGAIRRLTASLDPRLFRVNSIAAPNDIERKHHYLWRFWTRLPQLG
                     YMSIFDRSWYGRVLVERIEGFAKQDEWSRAYQEINEFERELTVSGAIILKYWLHITPE
                     EQKRRFTERQNDPLKRWKLTDEDWRNREKWPEYEQAAEEM"
     gene            complement(597..1349)
                     /locus_tag="PLG78_12905"
     CDS             complement(597..1349)
                     /locus_tag="PLG78_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24066.1"
                     /translation="MIRLRDYETGPELEKDSYDAAEKDLQERLYILFNECANRGLSCL
                     TILEGWAASGKGDALKTMTVRLDPRKFIVYTEGRGNSGYPFMYPYWQSLPYYGKAQFL
                     IGSWYSRAVDRHVKGKDSKQVFSENLESISNFERTICADNYTIFKFFLNISRDTQKSR
                     MKRAKKQGLSWIVARGDAKQNDRYAEYMEHYERILDKTDAGVSPWNIIAAENKYYTYF
                     TIMNTMVKSLEARLGVDSAAVMGQMKRTEEFA"
     gene            1475..2416
                     /locus_tag="PLG78_12910"
     CDS             1475..2416
                     /locus_tag="PLG78_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE24067.1"
                     /translation="MRIPEAPGRKSRALIVEGGGMRGSFAGGVLASMSRIYPASNFDI
                     VVGVSAGSCSAAYYVASTPAERLVEFCLDVWRYELTGSKFISYWNVLKGKTILNQEYL
                     IDYLFGKKYPIPKGRMDEPDVTPCYVTVTNLHTFLPEYVRATSSNLLSLLKAATSLPI
                     ATRGRKRLGEHEYTDGAVLDPMPIEAVMEAGYKDITVVLNHPRSHRSDPVGKMLSWLS
                     FPGNKTIRQMISREHHIRFNRAFDLINHPPTGFKVNIVDPDKPVAAGLVTRDMGRLNR
                     TVDHGIETGLRYFSELQKSRVFARFRDFFTRSSSGSR"
     gene            2413..3234
                     /locus_tag="PLG78_12915"
     CDS             2413..3234
                     /locus_tag="PLG78_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE24068.1"
                     /translation="MRLFGNWKFGRRPRIGVALGSGAARGWAHIGVLRCLEMNRIPVD
                     VVAGCSAGAVIGAFYAARAMTQVEKFALMYKGVRDTFKYLDFSPRSGGLVQGKNFIKF
                     LEEHLPVRNFSELKMPFGVVATDLVHMEEVHISDGPLLPAIRASVAVPGFLSPLESRS
                     PAGEVRQLVDGGLLNPVPVSLCRKLGADIVIAVDLNSEKFHEPAKSMTDIMARSIDAM
                     REVHKRNNFNFYPADVLVEPHMPEFGFFDYHRTQEAIDIGSKAAEEKLDRIKRLL"
     gene            complement(3218..4129)
                     /locus_tag="PLG78_12920"
     CDS             complement(3218..4129)
                     /locus_tag="PLG78_12920"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNE24069.1"
                     /translation="MANFSDSPFRKICRRMGSALSFSEFVPANSIAAGRPWALDMLRF
                     EEMERPVILQIFGNKLRSIEEAAIAVLERRPDVIDLNMGCSAREVALNGSGAGLLRTP
                     ELASAMIRSLVRIAGPEIPVSAKMRIGWDGFSRNYRDIALMIEDSGASMISVHGRTRE
                     EGYGGIADWDAIAEVKSMVRIPVIGSGDVSSRLDAENRIAQFGVDGVLVGRAAMGNPW
                     IFSGIRRSTLPREQITSVIKEHFLDMLTFYGDHAVILFRKHATRYAAEFPQHERLALL
                     SCETPSEFLDRLEECAQAEKLEATAIA"
     gene            4238..4507
                     /locus_tag="PLG78_12925"
     CDS             4238..4507
                     /locus_tag="PLG78_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24070.1"
                     /translation="MLPIQSSGHLSGAIVTWMLDRNRIEFTFDEFREMTAAIHSGGLF
                     KYLAGERPALLSQLTSIVRDKVPDIEEVEMEFVLEQALLNLQSRL"
BASE COUNT         1065 a         1107 c         1147 g         1228 t
ORIGIN      
        1 catttcctct gctgcctgct catactcggg ccatttttcg cgattgcgcc agtcctcgtc
       61 tgtaagcttc cagcgcttga gcggatcgtt ctgtcgctcc gtgaatcttc tcttttgctc
      121 ttccggtgta atatggagcc agtatttgag gatgatggca ccggacaccg tgagttctct
      181 ttcgaactca ttgatttcct gatacgcgcg agaccattca tcctgcttcg caaaaccttc
      241 tatgcgttcc acgagaactc gtccgtacca cgatcgatca aaaatgctca tgtatccgag
      301 ctgcggcaag cgcgtccaga atcgccacag gtagtgatgc tttctttcta tatcattggg
      361 cgcggcaatc gaattgactc gaaacagtct cggatccagg gaagcagtca gcctacgaat
      421 ggccccgcct tttccagcgg cgtcccaacc ttcgaatact acgatgatgg atctattttc
      481 atccgcagcc tgatgggaaa gtaggcgaac tttttcttga agatcattca gacttgcttt
      541 gtactcaggg cgttccaggg aaactgaatt gtccaggtct ttgaatcgaa gtacgctcac
      601 gcaaattcct ccgttctttt catctggccc atgacggctg cggaatctac cccaaggcgt
      661 gcttcgagag attttaccat tgtgttcatg attgtaaagt atgtgtagta tttgttttcc
      721 gcggctatga tgttccaggg agagacaccg gcatccgtct tgtccaggat tctttcgtag
      781 tgttccatgt attccgcata acggtcgttt tgctttgcgt ctccgcgtgc gacaatccag
      841 ctcagcccct gctttttggc tcgtttcatt ctggatttct gtgtgtcgcg agaaatgttt
      901 agaaagaatt tgaaaattgt gtaattgtcc gcgcagattg ttctttcaaa attggaaata
      961 gattccaggt tctcggagaa gacttgttta gaatctttac ctttcacgtg tctatccact
     1021 gcgcgactgt accaggatcc aatcaggaat tgcgcttttc cgtagtaagg gaggctctgc
     1081 cagtacggat acatgaacgg atagccgcta ttgcctctcc cttctgtgta gacgatgaac
     1141 tttcgcgggt ccaaccgcac agtcattgtt ttgagagcat ctcccttgcc gctggcggcc
     1201 cagccctcca ggattgtcag acaggatagt ccgcgattgg cgcattcatt gaatagaata
     1261 tagagtcttt cttgcagatc tttttctgcc gcatcgtagg aatctttttc tagctctggt
     1321 ccggtttcgt agtcgcgcag gcgaatcatg accgagaatt gatggtccgg ggaatatctg
     1381 tcaaacacaa aggccgcgaa ttcgagactc aaaagtactg gattttaaag ggcgctttcc
     1441 gaccatatgg ttagaagaga attctgaatc aaacatgaga atacctgaag cacccgggcg
     1501 aaagtcacga gcgttgattg tggaaggcgg aggaatgaga ggttctttcg caggcggcgt
     1561 tcttgcgtct atgagcagaa tttatcctgc atccaacttc gatatcgtag taggagtatc
     1621 cgcaggaagt tgctctgcgg cgtattatgt agcatccact cccgcggagc gcctggtgga
     1681 gttctgtctg gacgtatgga gatatgagct taccggaagc aaattcattt cttactggaa
     1741 cgtgctcaaa ggtaagacca ttctaaatca agaatacttg atcgattatt tatttggaaa
     1801 aaagtatccc attccaaaag gaagaatgga tgaaccggac gtgacaccct gttatgtgac
     1861 agtcaccaat cttcatacgt tcttaccgga atacgttcgc gctacgagtt cgaatttgct
     1921 gagcctgcta aaggctgcca cttctcttcc aattgcaaca cgtggtagaa agagattggg
     1981 ggaacatgaa tacacggacg gcgcggttct tgatccaatg cccatcgaag cagtgatgga
     2041 agcgggctac aaagacatca cggttgtatt gaatcatcca agaagccatc gttcggatcc
     2101 tgtaggaaaa atgctcagct ggttatcttt tcctggaaac aaaacaatca gacagatgat
     2161 ttcgcgcgaa catcacattc gcttcaatcg cgcctttgat ttgatcaatc atccgcctac
     2221 aggattcaaa gtgaacattg tggatccgga caaaccagtc gcggcgggtc ttgtgactcg
     2281 cgacatggga agattgaatc gtaccgtgga tcatggcatt gaaacgggcc tccgttattt
     2341 ttcagagttg cagaaatcac gcgtcttcgc gcggtttcgc gatttcttta cacgctcttc
     2401 atcaggctca aggtgagatt gttcggaaac tggaagtttg gacgcagacc gcgcattggc
     2461 gtggcccttg gttctggcgc ggcccgcggc tgggcgcaca tcggtgtctt gcgttgtctt
     2521 gagatgaatc gcattcctgt ggacgtcgtg gctggttgtt ctgcgggtgc ggtgattggc
     2581 gctttttacg ctgcgcgcgc catgacccag gtcgaaaagt tcgcgctgat gtacaaggga
     2641 gtgcgcgata cattcaagta tctggacttt tctccacgaa gcggtggact cgtacaagga
     2701 aagaatttta ttaagttcct ggaagagcat cttcctgtgc gaaatttttc agagctcaag
     2761 atgccgtttg gtgttgttgc aacggatcta gttcacatgg aagaggttca tatttcagac
     2821 ggtccacttc ttccagcgat tcgtgcgtct gttgcagtcc caggatttct atcccccctt
     2881 gaatcacgga gccccgccgg agaagtgcga cagcttgtag atggaggtct tctgaatccg
     2941 gtgccagtct cgctctgcag aaaactgggt gcggacattg tgattgctgt ggacttgaat
     3001 tctgagaagt ttcacgagcc tgcgaagtcg atgacggaca tcatggctcg ttccattgac
     3061 gccatgcgcg aagtgcataa gagaaacaat ttcaactttt atccggcaga tgtcctggtg
     3121 gagccgcaca tgcctgaatt tggattcttt gattatcaca gaacccagga agccattgac
     3181 attggatcaa aagcagcgga agaaaagctc gatcgaatca agcgattgct gtagcttcca
     3241 gtttctccgc ttgcgcgcat tcttccagtc gatccaaaaa ctctgatggc gtttcgcacg
     3301 atagtagagc cagccgttcg tgctgcggga actccgccgc ataacgggtc gcatgttttc
     3361 taaatagaat gactgcgtga tcgccataga aagtaagcat gtccagaaaa tgttctttga
     3421 tcacagatgt gatctgttcg cgcggaagag tagatcgcct tatccctgaa aagatccagg
     3481 gatttcccat ggccgctctt cccaccagca ctccgtctac tccaaactga gctatacgat
     3541 tttctgcatc gaggcgcgaa gacacatcac cggacccaat tacaggaatg cgaaccattg
     3601 attttacttc cgcgattgca tcccagtctg cgatcccacc gtatccttct tcgcgcgtgc
     3661 gaccgtgaac agaaatcatg cttgccccgc tgtcttcaat catgagagca atgtctctgt
     3721 agttgcgcga aaatccatcc caaccaattc tcattttcgc actcactgga atttctgggc
     3781 ccgcaattcg aacaagagag cgaatcatgg cagatgcaag ttcaggagta cgaaggagcc
     3841 ctgctcctga tccgttgagt gcaacttcac gagccgagca tcccatgttc aggtcgatca
     3901 catcaggacg tctttctaga acggcaatcg ctgcttcttc gatggaacgc agtttatttc
     3961 cgaagatctg gagaatgacc ggtctttcca tctcttcgaa tcggagcatg tccagggccc
     4021 atggtcttcc tgctgcgatg gaattcgcgg gaacaaattc tgagaacgac agagcggagc
     4081 ccattcttct gcaaattttg cgaaatggac tgtccgagaa attcgccata ggcgaaagga
     4141 tcatccgacc gtaaatgggg actcgaccta tgtgaaagga cggctgcacc ggctaagaat
     4201 cagttgagcc atgcgggcgg ccaatgtttt tcggtatgtg cttcccattc aatcgagcgg
     4261 tcacctcagc ggtgcgatag tgacatggat gctggaccga aaccgaattg agtttacgtt
     4321 tgacgagttc agagaaatga ccgcagcgat tcattccggc ggtttgttta agtacctggc
     4381 gggggaacgt ccagctctcc tctctcagct cacttcgatc gtgcgagaca aagtgccgga
     4441 tatagaagag gtggagatgg aatttgttct ggagcaggca ttgttgaacc ttcagtcgcg
     4501 tctgtgattc tctccggcga tttcttttgt tgttgtcatt gaatcag
//
LOCUS       DAOQJL010000308         4515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11476_length_4515_cov_4.717489, whole genome shotgun sequence.
ACCESSION   DAOQJL010000308 DAOQJL010000000
VERSION     DAOQJL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11476_length_4515_cov_4.717489"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLG78_12930"
     CDS             complement(<1..275)
                     /locus_tag="PLG78_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24071.1"
                     /translation="MRLYGIALLFVLAGSSLQAQSDPLDAVLSRASGDSSRLTLVNAT
                     DRTLETMVRFEGKFEETLLDARASMREGVESALLNPRAEDSGLETQDL"
     gene            complement(272..592)
                     /locus_tag="PLG78_12935"
     CDS             complement(272..592)
                     /locus_tag="PLG78_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24072.1"
                     /translation="MMQRVRGFFQSEEKIAWAIACVSLLFCLSVVVAVFTTGSSGRAA
                     ASTNENAKTDLLRQRMLLQARVNLCNLEKKSSTPVCDAGEQSRVVEYKKQIIEIDRKL
                     GESM"
     gene            complement(589..1128)
                     /locus_tag="PLG78_12940"
     CDS             complement(589..1128)
                     /locus_tag="PLG78_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24073.1"
                     /translation="MRPVIFVFSILLSACSAYRVESVRSGELQSGAREVAIGFFETYG
                     GRSDMARRNFYDGLSFSLREKGFRPAEAQEIKQLFDRAELPSERNLNPNELVRLASQY
                     PGRLFLQGRIDEVKTETLLEDRIQVMVSVSVYELKRGSKIAEIQVFGKDLEYRTGRES
                     LEMARLVSSELEKMAGLKP"
     gene            complement(1125..1730)
                     /locus_tag="PLG78_12945"
     CDS             complement(1125..1730)
                     /locus_tag="PLG78_12945"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE24074.1"
                     /translation="MRGAEILLILAMISCALSRASESRERAVVRSAASPSAWQTDMIA
                     VSGSDHSFYMDRFETGELSPGDYFSVRNQVPAVGMTRDEAEGICKRLEKRLCTMEEWR
                     TACLGVASRSHSYANAFVPGKCNVNGPDAIRTGERTECKSDLGIHDLVGNAMEWVSDS
                     VEGRGMALGGSFATGEKTDCFTAHYFARDGRSSQTGVRCCR"
     gene            complement(1717..2304)
                     /locus_tag="PLG78_12950"
     CDS             complement(1717..2304)
                     /locus_tag="PLG78_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24075.1"
                     /translation="MKFFAVCILFYSASLFAQEFVPGSDVIRESDILRRMEDEIGTPS
                     VSRTAHAAEQKKEPEKPEIPQITVHTGKLSRSGNSTLSQKEIVVSPDESLPVHIPMSD
                     VTEITFMKWTLRAAQDRRYYFPSVCKVRRKDGSEITGKMEYQDWLRLDVGGVSMLSYF
                     VSSTGGAEVEASAVPESVVRSLSFDTSENQESARR"
     gene            complement(2301..2888)
                     /locus_tag="PLG78_12955"
     CDS             complement(2301..2888)
                     /locus_tag="PLG78_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24076.1"
                     /translation="MIRLLPLIFLVTFCAKPVAGFYSKLHVDDNKGPVIVKSAHYDLH
                     RDSFSYAVASDHVVTMKDALEMPSREKMLLFKRAFLRKAAIQSGLKEGIFDSEEAQQY
                     ILPRLESILEDYYYYEKARPDRIKRSLSSLNVDDESLAALLKEDPALARVASPEKLRH
                     EKDRILERLYELRMAQAKKQAMENLLKTLDPQVVL"
     gene            3168..3788
                     /locus_tag="PLG78_12960"
     CDS             3168..3788
                     /locus_tag="PLG78_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24077.1"
                     /translation="MKYIRHNLALVPVFFVFTCAHQELSEQEALQLKQAVTEYREQKF
                     DQAETTLTELGKKFPANIEINVLRARIKYYTRDFAASESILRDLLKKREGNAHASIWL
                     GRVVAVSPKRTEEERLNEASSIFKQVLKDDPENFQAHYLLGRCLEAQGKLELALYEYQ
                     AAEIPLEDQAQRINAHLGDLFTRLNMTDRAAKHRKMAGALAGSQEK"
     gene            3854..>4515
                     /locus_tag="PLG78_12965"
     CDS             3854..>4515
                     /locus_tag="PLG78_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE24078.1"
                     /translation="MSLREAEIIAEENHPELRALRQERAIANENIKVKFRDFFPSVSL
                     SYRQSRTVAQREFDNGSQSIQLNISQPIYDGGRTSLAHEVAKLDASISTEKYKKARND
                     LKLRVRTAFFALQQQKGQMDLARLSIKSTDLLYKRAVIERRQDAIALIDFREIENEHK
                     RRQFALEKQEAAYRSSIADFAHTLHLPPGSEPALVAIDLTRTVIRDSNRLPDELKVLA
                     EE"
BASE COUNT         1234 a         1225 c         1035 g         1021 t
ORIGIN      
        1 aggtcttgcg tttcaagacc tgaatcttcc gcgcgcggat tcaatagggc cgactctact
       61 ccctctcgca tactcgcgcg tgcatcgagc aaagtctctt cgaatttacc ctcaaagcga
      121 accatcgttt caagagtgcg atctgttgca ttgaccagag tcagtcgaga tgaatctcca
      181 ctggcgcgag aaagcaccgc gtcgagtgga tccgactgcg cctgcaagga agacccggct
      241 aaaacaaaaa gaagtgcaat tccataaagt ctcacatgct ttctcccagc ttacggtcga
      301 tttcaatgat ctgctttttg tattctacca cgcggctctg ttcgcccgca tcgcagactg
      361 gcgtggatga tttcttttcc aggttgcata gattcactcg cgcttgcaaa agcattctct
      421 ggcgcagcaa gtctgtcttc gcattttcgt ttgtggaagc cgccgctcgc ccggaagaac
      481 ctgtcgtgaa cacggccacc acaacgctca gacaaaacag caaagaaaca catgcaatcg
      541 cccaggcgat cttctcttcg ctttgaaaga atccgcgcac tcgctgcatc atggtttcag
      601 tcccgccatc ttttcgagtt ctgaagaaac gagcctggcc atttccaggg attcacggcc
      661 tgtgcgatac tccaggtcct tgccaaacac ctggatctca gcgatcttgc tgcctctctt
      721 gagctcgtac acactcacac tgaccatcac ctggatgcga tcctcaagaa gtgtttctgt
      781 ttttacttca tcaattcttc cctgaagaaa gagtcttccc ggatactgag acgcaagacg
      841 cacgagctcg tttggattca gatttctttc agatggaagc tccgcgcgat caaagagttg
      901 tttgatttcc tgagcttccg ctggacgaaa gcctttctcg cgcaaagaaa aagacaatcc
      961 atcgtagaaa ttgcgacggg ccatgtccga tctgccgcca taagtttcga aaaacccaat
     1021 ggcaacttcg cgcgcgccag actgcaattc gccagagcga accgattcca ctctgtaagc
     1081 gctgcatgcg gaaagtaaaa tcgagaaaac aaaaatgacc ggtctcatct gcaacacctc
     1141 actcctgtct gagaggatct tccatctcgc gcaaaataat gcgccgtaaa acaatccgtt
     1201 ttttcccccg tggcaaacga accaccaaga gccatacctc tgccttccac agaatcggac
     1261 acccactcca ttgcatttcc tacaagatca tggattccaa gatctgattt acactctgtg
     1321 cgttctccag tgcgaattgc atccggtccg tttacattgc acttaccggg cacaaacgca
     1381 ttcgcgtagc tatgcgaacg agaggccacc ccgaggcagg cagtcctcca ttcctccatc
     1441 gtacacagac gcttctcgag cctcttacaa ataccttccg cttcgtcgcg ggtcatgcct
     1501 accgcaggaa cctggtttct gacagagaaa tagtctccag gtgatagttc tccggtttca
     1561 aatcgatcca tgtaaaaaga atgatcagag ccagacacag caatcatatc agtctgccag
     1621 gcgctgggag acgccgcaga tcgaaccaca gcccgttcgc gtgattcact cgcgcgcgac
     1681 agagcgcaag aaatcatagc gagtatcaac aaaatctcag cgcctcgcac tttcctgatt
     1741 ctccgacgtg tcaaaagaca aactgcgaac cactgattca ggtacggcgg acgcttctac
     1801 ttccgcgccg ccggtggaag atacaaaata ggatagcatg gaaactcccc ccacgtccag
     1861 gcgaagccag tcctgatact ccatcttgcc cgtaatctca gatccgtctt tgcgtcgaac
     1921 tttgcatacg gacggaaagt agtacctgcg atcctgggcg gctcgcaaag tccatttcat
     1981 gaaagtaatt tctgtcacat ccgacatggg aatgtgcacc ggcagcgatt catccggaga
     2041 aaccacgatt tccttctggc tgagagtaga attcccgctg cgagacagtt tgcctgtgtg
     2101 cacagtaatc tggggaattt cgggcttttc tggctctttt ttctgctcag ctgcgtgcgc
     2161 cgttctcgaa acggaaggtg tgccgatttc atcttccata cgacgcaaaa tatccgattc
     2221 gcgaatcaca tctgaccctg gaacaaattc ctgggcgaac aatgatgcag agtagaacag
     2281 aatgcagact gcgaagaatt tcataacacc acctgcggat ccaatgtctt cagcaagttc
     2341 tccatagcct gcttctttgc ctgggccata cgaagttcgt acaatcgctc gaggattctg
     2401 tccttttcat gacgcaattt ctctggactg gccacgcgcg ccagggccgg atcttctttt
     2461 agaagtgcgg caagagactc atcatctaca ttcaaagaag agagagaccg tttgatccta
     2521 tctggccgcg ccttctcata gtagtaataa tcctccagaa tggactcgag acgtggcaaa
     2581 atgtactgct gcgcttcctc tgaatcaaaa atcccctctt tcaatccgga ttgtatggcc
     2641 gcctttctca aaaaggcgcg cttgaataga agcatcttct cgcgcgaagg catttccaat
     2701 gcatctttca ttgtgaccac gtgatccgaa gcgactgcgt aactgaaaga atcacgatga
     2761 agatcgtaat gagcagattt gacgatcact ggacctttgt tatcgtccac atgaagcttc
     2821 gaataaaacc ctgcgacagg ttttgcgcag aatgttacaa gaaaaatgag agggagcaat
     2881 cgaatcacaa tggcaagagg atgaagcaga gaacacagtc aaatacaaac tgatgaaaga
     2941 gcgcgaatca ttaaaaaaat ccgaaagaac gttcggaaca agacgcggca acagaaacca
     3001 acagaacgtt agtcgcccag ggacgaagag caagtgttgt tcattccaaa atacatacct
     3061 ttcattccaa acgctcccgg atcgtaggca cgatttcgat ttttatcaca atctatttga
     3121 cacacatcac tgaaattttt tctccgatgc ctcggatcaa aatagttatg aaatacatac
     3181 gacataatct cgcactcgtg cctgtctttt tcgtgttcac ttgcgcacat caggaacttt
     3241 cagagcaaga agcgttgcaa ctcaagcaag ctgtgacaga atatcgcgaa cagaagtttg
     3301 atcaggctga gactactcta acggaactgg gaaaaaaatt tcccgctaat atagaaatca
     3361 atgtattgcg tgctcgaatc aagtactaca cacgcgattt cgccgcatcg gagagcatac
     3421 tgcgagatct actcaagaaa agagagggaa atgcacatgc ttccatctgg ctcggtcggg
     3481 ttgttgcggt cagcccaaag agaacggaag aagaaagact gaatgaagca tcctccatct
     3541 tcaaacaagt cttgaaagac gatcccgaga acttccaggc gcactatctg ctcggccgat
     3601 gcctcgaggc gcaaggaaag ctggagctgg cgctttacga ataccaggca gcggagattc
     3661 cgctcgaaga ccaggctcag agaatcaacg cgcatctggg ggatctcttt accaggctca
     3721 acatgacgga tcgcgcagca aagcatcgca agatggcggg cgcgctcgct ggaagtcagg
     3781 agaagtaagt tgtccccgct cgtgcaactt ggggtgaccg tcctttttct tccagacgtc
     3841 tcccgcgccg cagatgagtc tgcgcgaagc ggaaatcatc gcagaagaga atcatccgga
     3901 gctcagagca ctgcggcagg aacgcgctat cgcaaatgag aacatcaagg taaaattcag
     3961 agacttcttt ccttctgttt cgttgagcta tcgccagagc agaacggtag cgcagagaga
     4021 attcgacaac ggctctcagt ctatccagtt aaacatttca caaccaatct acgacggagg
     4081 caggacgtct ctggcccatg aagtggcaaa gctggacgca tccatcagca ccgaaaaata
     4141 caagaaagca cgcaacgatc tgaagcttcg agtcagaact gccttcttcg ctctgcagca
     4201 acaaaaagga caaatggacc tggcgcgcct ctcgataaaa agcactgact tactgtacaa
     4261 aagagccgtt atcgaacgaa gacaggatgc aattgcgctc atcgactttc gtgaaataga
     4321 aaacgaacac aaacgccgcc agttcgcgct ggaaaagcag gaagccgcct atcgttccag
     4381 catcgctgat ttcgcgcaca ccttgcatct acctcctgga tcggaacccg cgctcgtagc
     4441 catagatctg accagaacag tcattcgaga ttcaaacagg ctgccggacg agctcaaggt
     4501 gctggcagaa gaaaa
//
LOCUS       DAOQJL010000309         4478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11638_length_4478_cov_4.093828, whole genome shotgun sequence.
ACCESSION   DAOQJL010000309 DAOQJL010000000
VERSION     DAOQJL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_4478_cov_4.093828"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLG78_12970"
     CDS             <1..791
                     /locus_tag="PLG78_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24079.1"
                     /translation="FMHSTPSVCADCSLGCNTRVDADKQNKIKRIIPRRNNEVNKEWM
                     CDHGRLSFPYVYENRMNLPTKNNQEIPWADAFRMAAEALGGNDVVFLVSLWTTLEGME
                     KIKSLAAEKGSPVFGFGNASTKDQVFPGFTIYGDKNPNRAGFTATFGDLKSPSASDLN
                     GKTVFVFGNVPRFEIPADLAQSLTSASKLIVVDFARGSFANHAKTQLVLSGLTHFEKN
                     GTFVNRGGLRQGFSPVIEPVGFGRSEVELLDGILKERGKTAAAR"
     gene            798..1826
                     /gene="nuoH"
                     /locus_tag="PLG78_12975"
     CDS             798..1826
                     /gene="nuoH"
                     /locus_tag="PLG78_12975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNE24080.1"
                     /translation="MEQLVFIVFKCVTAVVLVLTAVPLMIWMERKVSADFQLRIGPNR
                     MGPAGVFQLPVDAVKLLFKESFAPRGIDRFMYLAAPVVAFVPGLLNFAVIPAGEIRAE
                     QGGLLLAVSDIELGLITILAISSLTSYGIAYAGWASGNQFSLLGGVRSCAQLISYEIV
                     LGLSVLSVIMMSGTLNLSEIVRMQNQLRFGWMPSWNIALLPLTFILFLIAAFAETNRA
                     PFDLPEAEQELVGGYHTEYTGLRYAWFMFGEYAGMFTMCSLITTLFLGGWMLPGLTLP
                     LGILGGILGFGIFLTKVIALIFFFMWVRWTLPRLRWDQLMRFGWKRLIPAGLINLVIT
                     AVLVQVWK"
     gene            1840..2370
                     /locus_tag="PLG78_12980"
     CDS             1840..2370
                     /locus_tag="PLG78_12980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNE24081.1"
                     /translation="MKQAVPYATTLGSIKALIQGLYVSMGQFLKKPVTIQYPEVQAPI
                     DPRFRGEHALTKDEFGHMKCVACYMCATACPAECIHIEAQPAPEGEGWEGRDKIPQSF
                     DIDMLRCIYCGYCVEACPKDAIMMTNKVPVVHDNRKAFIYGIDKLLGNFEEYGRDLHT
                     KQLKERGIEEPGHAGH"
     gene            2357..2917
                     /locus_tag="PLG78_12985"
     CDS             2357..2917
                     /locus_tag="PLG78_12985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE24082.1"
                     /translation="MQVTEIIFLINAAFLVLFSLAVVVIKNPVRATFMLILSFLPTTA
                     IYFFLHAPFVGILQILVYAGAILVLFTFVVMMVNPAPGDGEISPDGAKRLPRSRIAAV
                     GIVLLLGGVLLSVVLSISFPAIASVPAEFGGLRAIGTMIFKNPSENPYIFSFELLSFL
                     VLAGIIAAVSIARGREHHRHGPGKPQ"
     gene            2914..3246
                     /gene="nuoK"
                     /locus_tag="PLG78_12990"
     CDS             2914..3246
                     /gene="nuoK"
                     /locus_tag="PLG78_12990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE24083.1"
                     /translation="MSVFSLQTCLILSSLLILIGCYGIIIRRSLLLIFLSVEIVLNGV
                     HVALVAFNYFRWSGLEAGHYIYMLSIGVAAVEAAVGLSMIILIFKNSGTILSDALTKL
                     GEDKEENK"
     gene            3243..>4478
                     /locus_tag="PLG78_12995"
     CDS             3243..>4478
                     /locus_tag="PLG78_12995"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE24084.1"
                     /translation="MNAALPWIPLLPALGAGLCAIGGRRLGLRGSSFIVVTASLVSLV
                     LCCLIAFQGVIAGNIFQEGKSEVFNAGPFLQFSEEWSALLGLRMDLMSGLMSLLVTLF
                     GTLILMFSIDYMREDDSPVRYFASLSLFISAMLILVLSENLVLLFLGWEGVGLCSYLL
                     IGHEWKKSGVPLAAYRAFFVNRIGDLFFILGVFLLLKTLGTVSFSGMSQKFETMSGSL
                     DPLARRDLLMASFLLLGGAFGKSAQVPLHVWLPDAMAGPTPVSALIHAATMVTAGVFL
                     VARLNWLYTAIPEARGLLLICGSLTLLVGAVLACFQSDIKKVLAYSTLSHLGLMFIAL
                     AAGSVSAGLFHLFGHASFKAISFLAAGSIILAAHHEQDLHKLRGSLKHLPVTRVALWT
                     GAIGGAGLVPFLASGFFSKE"
BASE COUNT          918 a         1068 c         1164 g         1328 t
ORIGIN      
        1 ggttcatgca ttctactcca tctgtttgtg cggattgttc tctcgggtgc aacactcgtg
       61 tggacgcgga caaacaaaat aaaatcaaga gaatcattcc tcgtcgcaac aatgaagtaa
      121 acaaggaatg gatgtgcgat catggtcgtc tcagcttccc atacgtttac gaaaaccgaa
      181 tgaacctccc aacaaaaaac aatcaggaga tcccctgggc ggatgcgttc cgcatggcag
      241 cggaagcgtt aggcggcaat gatgtcgtgt ttctagtaag tctctggacc actctcgaag
      301 gaatggaaaa gatcaaatcc ctcgcagcgg aaaaaggatc acctgtcttt ggctttggca
      361 atgcgtccac aaaagatcaa gtcttccctg gttttacaat ctacggagac aagaatccaa
      421 atcgtgctgg cttcactgcc acatttggag atctgaaatc tccttctgca tctgatttga
      481 atggaaagac agttttcgta tttggaaatg ttccgcggtt tgagattccg gcagatctgg
      541 cgcagtctct aacctctgca tcaaagctca tcgtggttga ttttgccaga ggctcgtttg
      601 caaatcatgc gaagactcaa ctcgttctct cagggctcac tcattttgag aagaatggaa
      661 catttgtgaa tcgtggtggg ttgcgacagg gattttcccc tgtgattgaa cccgttggat
      721 ttggcagaag cgaagtggaa ttgctggacg gaattttgaa agaaagggga aaaacggctg
      781 cggctcgttg atttagtatg gagcagcttg tattcatcgt attcaagtgt gtaactgcgg
      841 ttgtcctggt cttgactgcc gttcctttga tgatctggat ggagcgcaaa gtcagcgctg
      901 acttccagtt gcgcattggt ccaaatcgca tgggacctgc cggtgtgttt caacttccag
      961 tggatgccgt gaaacttttg tttaaggaat catttgctcc acgcgggatt gatcgcttca
     1021 tgtatctggc ggcacctgtg gttgcctttg ttcctggtct gctcaatttc gctgtaattc
     1081 ccgccggaga gattcgtgcg gagcaaggag gtcttcttct tgctgtctct gacatcgagc
     1141 tcgggctcat taccattctt gctatttctt cgctaacgtc atacggcatt gcctacgccg
     1201 gatgggcatc cgggaatcag ttttcccttc tgggaggagt tcgatcctgc gcgcaactca
     1261 tcagctacga aatcgttctt ggtctatctg ttctttctgt catcatgatg agcggcacac
     1321 tcaatctcag cgagattgtt cgcatgcaaa atcaacttcg ctttggctgg atgccttcct
     1381 ggaatattgc tcttcttcca cttactttca ttttgttctt gattgccgct ttcgcagaaa
     1441 ccaaccgcgc gccatttgat cttccggaag cggaacagga actcgttggt gggtatcata
     1501 cagagtacac cggtctgaga tacgcctggt tcatgttcgg agaatacgcg ggaatgttta
     1561 cgatgtgttc cttgatcacc actctgtttc ttggtggttg gatgctcccc ggactgactc
     1621 ttccacttgg gattctgggc gggattctgg ggttcggaat ttttctcacc aaggtgattg
     1681 ctctaatttt ctttttcatg tgggtgcggt ggactctacc gcgcttgcgc tgggatcagt
     1741 tgatgcgttt tggctggaag cgtttgattc cggccgggct catcaatctc gtgatcacgg
     1801 cagtgctcgt tcaggtctgg aagtgaaggc ggatcaagta tgaagcaagc agtgccgtat
     1861 gctacaactc tgggatctat caaagcgctg atccagggtc tgtacgtttc catgggtcag
     1921 tttctgaaga aaccagtcac cattcagtat ccagaagttc aggcacccat tgatcccagg
     1981 tttcgcggag agcatgctct cacgaaagat gaatttggcc atatgaagtg tgtggcctgc
     2041 tacatgtgcg ccactgcctg ccctgctgaa tgcattcata tagaagcaca acccgctccc
     2101 gagggagaag gctgggaagg cagagacaag attcctcagt cttttgacat agatatgctt
     2161 cgctgcattt attgcggcta ctgtgtggaa gcatgtccaa aggacgccat catgatgacc
     2221 aacaaagttc cagtggttca tgacaatcgc aaagcattca tctacggaat cgataagctg
     2281 cttggaaatt tcgaagagta cggtagagac cttcacacga agcaactgaa ggaacgaggc
     2341 attgaggagc caggccatgc aggtcactga gatcatcttt ctcatcaacg cggcatttct
     2401 tgttttgttc tccctcgctg tagtcgtgat caagaacccg gtgcgcgcaa cctttatgtt
     2461 gattctgtcc ttcctgccca ccactgcgat ctacttcttt ttgcatgcgc cctttgttgg
     2521 aattttgcaa atcctcgttt acgcaggcgc gattctggtt ctgttcactt ttgttgtcat
     2581 gatggtgaac cccgcacctg gagacggcga aatttcgccg gacggtgcaa agcgcttgcc
     2641 tcgaagcagg atcgcagctg tgggtattgt tctgcttctg ggtggtgtgc ttctgtctgt
     2701 agttttgagt atttcgtttc ctgcgattgc aagcgtgcct gcagaatttg gcggccttcg
     2761 cgccatcggc acaatgattt tcaagaatcc ttctgagaat ccctacatct tctctttcga
     2821 gttgttgagc ttcctggttc ttgcgggaat cattgccgct gtcagcatag caagagggag
     2881 agagcatcac agacacgggc cgggaaaacc acaatgagcg ttttttctct ccagacatgt
     2941 ttgattttat catcgctttt gattctgatt ggttgctacg gcatcatcat tcgcagaagt
     3001 ttgcttctga tttttttgtc tgttgagatc gttctcaacg gcgtgcacgt tgcactggtt
     3061 gcttttaact acttccgctg gtcgggattg gaggcaggtc actacattta tatgctttcg
     3121 atcggcgtcg ccgctgtgga agcggcagtt ggtctttcta tgatcattct cattttcaaa
     3181 aactcaggta cgattttgtc agacgcactc acgaaactgg gcgaagacaa agaggaaaac
     3241 aaatgaacgc ggcattgccg tggattccac tccttcctgc tctgggcgcg gggttatgcg
     3301 ccataggcgg caggaggctg ggccttcgtg gatcctcatt cattgtagta accgcttcgc
     3361 ttgtctctct tgtgttgtgc tgcctcatcg cttttcaggg tgtcatcgcg ggcaatatct
     3421 ttcaggaagg aaagtccgaa gtatttaatg cgggcccatt ccttcagttt tcggaggaat
     3481 ggtctgcgct actgggcctt cgcatggatc tgatgtctgg cctgatgagt ctccttgtaa
     3541 cgttattcgg cactctgatc ctgatgttct ccattgatta catgagagaa gatgattcgc
     3601 ctgttcgcta tttcgcaagt ttgtctttgt ttatctctgc catgttgatc ctggttctgt
     3661 ctgagaatct ggttcttctg ttcctgggat gggaaggggt tggcctttgc agctacttgc
     3721 tgatcggaca tgaatggaaa aaatcaggcg tgcctctggc cgcgtatcgc gcatttttcg
     3781 taaaccgaat tggagatctc ttttttatcc tgggagtttt tcttctactt aagactctgg
     3841 ggaccgtttc attttccgga atgtctcaga agttcgaaac catgtccggg tcactggatc
     3901 cgttggcgcg aagagatttg ttgatggcga gtttcctgct tctgggtggc gcgttcggta
     3961 agtcggcaca ggttcctctt cacgtctggc ttcccgatgc aatggctggt cccacaccgg
     4021 tcagtgctct gattcacgcg gccacgatgg tgacggctgg cgtgtttctg gtggctcgtt
     4081 tgaactggct ctacactgcg attccggaag cgcgcggatt acttctcatc tgcggctcac
     4141 tcacattgct tgtgggcgcg gtactcgcat gctttcaaag cgacatcaag aaagttctgg
     4201 cctattccac gctttcgcat ctggggttga tgttcattgc tctggcggcg ggttctgttt
     4261 ccgcgggtct gtttcatttg ttcggccacg cttctttcaa ggcgatttct tttctggccg
     4321 cgggaagtat catccttgcg gcacatcatg aacaggatct gcataaactg cgcggatctc
     4381 tgaaacatct ccctgttact cgcgttgcgc tctggactgg agcaatcggc ggtgcggggc
     4441 tggttccatt tctggcctct ggttttttct ccaaagag
//
LOCUS       DAOQJL010000310         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11643_length_4475_cov_4.793213, whole genome shotgun sequence.
ACCESSION   DAOQJL010000310 DAOQJL010000000
VERSION     DAOQJL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_4475_cov_4.793213"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLG78_13000"
     CDS             complement(<1..1735)
                     /locus_tag="PLG78_13000"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNE24085.1"
                     /translation="MAVSQDIICPFQTVPSLLEARAKETPQKPYLIFQEEEVSYSQFR
                     SSVRKMAAHFQTTGAGHGSRVAILIPNSDHFLYCYFGAMEAGLAAATLNTLLKAEELE
                     FIINDCEAEILCTTPHYRKLLDPVFENLKNIKHVIFTEPGDLPHSALIGDILKGNAEY
                     SDPNVTAADDAAMIYTSGTTGHPKGVVLTHSNVMYNSYVAPRFIDLQAADVSLCIMPL
                     FHVNAQIASMLATMQAGATVVLEEMFKPRTFIKTLKKHRCTTFSGVPTIFNFLNEMKE
                     AEGEDLSFLKACICGAAPMPVEVFQTFERKFGAKIIEGYGLSEGTCVSSLNPLNGVRK
                     IGSIGIPIDGQDMSIWDDDANEMPAGQTGEIVIKGPNVMKEYYRNPEATAKTLHNGWL
                     RTGDLGYRDQDGYYFIVGRKKEMIIRGGENIYPREIEEVLYENPEVQDCAVVGLPDKK
                     YGEEVAAFIILKGGSSAYEKTIKSYLRAKIADFKRPRIIEIVTELPRTATGKIQKNKI
                     IEEFVGNQELISRVNGKVLIPYRWVYGKALSRFYTGLREEGKFYGTARKGCEKCNGAV
                     QLPPKSFCGLCH"
     gene            1840..2136
                     /locus_tag="PLG78_13005"
     CDS             1840..2136
                     /locus_tag="PLG78_13005"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNE24086.1"
                     /translation="MFEMMVAMDIGDEEAYSAYRAGMSPILKSFGGRFRYDFKVSEAL
                     QREATHPINRVFALNFPDRISRDAFFSNPDYKKVRSAYFEQAVKARTLIAEYDL"
     gene            complement(2146..2595)
                     /locus_tag="PLG78_13010"
     CDS             complement(2146..2595)
                     /locus_tag="PLG78_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24087.1"
                     /translation="MEVKVLEGKIENDYKWNPGTAVGRFLTQMREKEILAVRCTKTSK
                     VFLPPQSWSPYGNIKMDRFIPITAEPKLGAGTIVYQAPWNMPEDLKPPYMLAGISFAG
                     ADSDLIHLVVAPEADLKALKPGDRLRAVWKAETTGTIRDILYFEPVK"
     gene            complement(2604..3812)
                     /locus_tag="PLG78_13015"
     CDS             complement(2604..3812)
                     /locus_tag="PLG78_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24088.1"
                     /translation="MPQKVAITGIGQTRTRLKRKDVNFPELMGEAAQLALQDAGLNAE
                     DIDLVLYGSGPEMFEGVGEPELWGADNTFGVFRPHYRIQTGGTVGGSTTIAGWYMVNS
                     GLANNVLVVSGNKLGESSVQKGLSLVYSPTMGRDFAAGAPSAVANQTRIYMNKYPEAK
                     EEQFARVGTMMRKNALNNPNAALKLPQISEEMMMNMAWLSTPFRLLDSCPTSDAACAM
                     VLQGEKNAEANDRPRAWIQAVSAVSDGVNYINRDWSDPIALKKAAQNCYKAVGITKPL
                     EELDVVEIYDAFTSQHLIWYEGLGLCAPGRAGPDLIETRATSMTGQLPVNPSGGVLSN
                     NSIGASAMIRQAEVALQLMGRAGDRQVANAEVGLAHGWGGAIQFHVVMIMSKEKEIQE
                     SFRKRKEKGR"
     gene            complement(3853..>4475)
                     /locus_tag="PLG78_13020"
     CDS             complement(3853..>4475)
                     /locus_tag="PLG78_13020"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE24089.1"
                     /translation="KVGGDLYDIRRNGDSLEIFVVDVSGHGVSSAFLAAIFRVSMESA
                     DRSNPAAFLSSMNEIFFDRSFTGFFLTAFYGVLDLRSLTLRFARAGHPHPLLVRNGQV
                     QEVAGKGGMMGIMRQLVLEDATLQLRKGDRLMVYTDGIPEYQTSDGVLFGEENLYSVL
                     IEGQNEPVSRLPTLLLSRLGEFAQDRSAEDDMTMVVFDISSDFKLS"
BASE COUNT         1073 a         1162 c         1073 g         1167 t
ORIGIN      
        1 catgacagag tccgcaaaat gatttgggcg gcagctgtac tgcaccattg catttttcgc
       61 aacctttgcg agcggtgccg taaaactttc cttcttcgcg aagccccgtg tagaaacgcg
      121 aaagcgcttt tccatacacc cagcgatacg gaatcaagac cttgccattt acgcgcgata
      181 tgagttcctg gttgcctaca aactcttcaa tgattttgtt cttttggatc tttcctgtcg
      241 cagtgcgcgg caactcagtt acgatttcga tgatgcgtgg tcgtttgaag tctgcaattt
      301 tggcgcgtaa gtagctcttg atggttttct cgtatgcgga agatccgccc ttcaaaataa
      361 taaaagcggc tacctcttct ccgtatttct tgtcgggcag tccaaccact gcgcaatcct
      421 gcacctccgg attttcatag agaacttctt cgatttcgcg cgggtaaata ttctctccgc
      481 cgcgaatgat catttctttc ttgcgcccta caatgaaata gtaaccgtcc tgatcgcgat
      541 agccaagatc ccctgtcctg agccagccat tgtgaagcgt cttagcagtt gcttcaggat
      601 tacgatagta ctccttcatc acgtttgggc ctttgattac aatttcgcca gtttgtcctg
      661 cgggcatctc gttggcatca tcgtcccaga tgctcatatc ctggccatca attggaattc
      721 caatagagcc gatctttcga acgccgttta acgggttcaa agacgagaca caggtgcctt
      781 ccgaaaggcc ataaccttcg atgatcttcg cgccaaactt acgttcaaac gtttgaaaga
      841 cttctaccgg cataggagcg gcgccgcaaa tacaggcttt caagaagctg agatcttccc
      901 cttccgcttc cttcatttca ttcaagaaat tgaaaatggt aggtacgccg ctgaatgtag
      961 tgcaacgatg cttcttgagt gtcttgatga atgtccttgg cttgaacatt tcttcgagta
     1021 caacggtagc gcctgcctgc atggtggcga gcatggacgc aatttgcgcg ttcacgtgaa
     1081 aaagaggcat gatgcacagc gacacgtccg ccgcctggag atcaatgaag cgcggtgcca
     1141 catagctatt gtacatcaca ttggagtgcg tcaacaccac gccctttgga tggcccgtgg
     1201 tcccagacgt gtaaatcata gccgcatcgt cagcagcagt cacatttgga tcagagtatt
     1261 ccgcatttcc tttgagaata tctccgatga gagcggaatg aggcaaatcc ccaggttcag
     1321 tgaagatcac atgcttgata ttctttaagt tttcaaaaac aggatccaga agttttctgt
     1381 agtgaggcgt ggtgcacagt atttctgctt cgcaatcgtt gatgataaat tcgagttcct
     1441 ctgccttcaa aagtgtattg agtgtcgcgg cggcgagtcc tgcttccatg gcaccaaagt
     1501 aacaatacaa aaagtgatcc gagttcggga tgagaatggc aactctcgat ccgtggcctg
     1561 ctcccgtagt ttgaaagtgt gccgccatct tacgaacaga agagcgaaat tgggaatagc
     1621 ttacttcttc ttcctgaaaa atgagatatg gtttttgcgg agtctccttc gcgcgtgctt
     1681 ctagaagaga aggaactgtt tgaaaaggac agatgatgtc ttgactgact gccatttcga
     1741 aaacatggga ttcgtcctca ggcagtcacg tcctttttca aaggaacccg tgaatgtagg
     1801 cgatgcttga ctctgcctca cactcaacgt ctctggcgta tgtttgaaat gatggtagcc
     1861 atggacattg gagatgagga ggcgtattct gcataccgtg ccggaatgag tccgattctg
     1921 aaatcgttcg gaggccgctt tcgttacgat ttcaaggtga gcgaggcact gcaaagagaa
     1981 gcgactcatc cgatcaacag ggtatttgct ttgaattttc ccgaccgtat ctcacgggac
     2041 gctttcttct cgaacccgga ttataagaaa gttcgatccg catacttcga acaggctgta
     2101 aaggccagaa ccctgatcgc ggaatacgat ctctaattca gccgatcact tcacaggttc
     2161 aaaatacaga atgtcgcgga tggttcctgt ggtctctgct ttccagacag cgcgcaacct
     2221 gtctcccggt ttgagggctt tgagatctgc ctccggtgcc accacaagat gaatcaggtc
     2281 gctgtccgcg cctgcgaatg agatgcccgc gagcatatag ggtggtttga gatcttctgg
     2341 catgttccag ggagcctgat acacaatcgt gcctgctccg agttttggct ctgccgtgat
     2401 gggaatgaag cgatccatct taatattgcc gtacggagac caggattgcg gcggtagaaa
     2461 cactttcgaa gtctttgtgc atctaaccgc gagaatttcc ttctcacgca tctgggtcaa
     2521 aaaacgacca actgccgtgc ccggattcca tttgtaatcg ttttcgatct tgccttcgag
     2581 tacttttact tccatatgat cgcctaacgt cctttctctt tgcgtttgcg gaacgattcc
     2641 tgaatttctt tttccttgct catgatcatg acaacgtgga actgaatggc accgccccat
     2701 ccgtgcgcga gaccgacctc tgcattcgcc acctgcctgt cccctgcacg gcccatcaat
     2761 tgaagagcaa cttctgcctg acgaatcatc gcggaggcgc cgatggaatt gttggacaag
     2821 acaccgccgc tcggattcac cggaagctgg cctgtcatac tggtggcgcg agtttcaatc
     2881 aaatcagggc ccgctctccc tggagcgcat agtccaagtc cttcatacca gatcagatgc
     2941 tggcttgtga atgcatcgta aatttccact acgtccaatt cttcgagagg ctttgtaatg
     3001 ccgacggcct tataacaatt ttgcgccgct ttcttgagag caatcggatc cgaccagtcg
     3061 cgattgatgt agttcactcc atctgaaaca gctgacactg cctgaatcca cgctcgcggg
     3121 cgatcgtttg cttctgcatt cttttctccc tgaaggacca tcgcacaggc ggcgtcgctc
     3181 gtcgggcaag aatccagaag acgaaacgga gtagaaagcc atgccatgtt catcatcatc
     3241 tcttctgaaa tctgaggaag tttgagcgca gcattcggat tgttcagagc attcttgcgc
     3301 atcatggttc caacgcgcgc gaattgctct tctttcgctt ccggatactt gttcatataa
     3361 atgcgcgtct gattggcaac ggcagaagga gcaccggcag caaagtcccg acccatggtg
     3421 ggagaataca caaggctcaa acctttttga accgatgatt ctcctaattt atttccagaa
     3481 acaactagaa cgttattcgc caatccagag tttaccatgt accaaccggc gatcgtggtg
     3541 gaaccaccta ctgttccgcc agtctggatg cgatagtgtg gacgaaaaac gccaaacgta
     3601 ttgtccgcgc cccagagttc tggttcgcct acgccttcga acatttcggg tccgcttcca
     3661 taaaggacca gatcaatatc ctctgcattt aaccctgcgt cttgcaaggc aagctgtgct
     3721 gcttctccca tgagctcagg gaaattcaca tcttttcttt tgagtctggt tctagtctga
     3781 ccgatgccgg tgatggcaac cttttgaggc atgagcgaat ctcagcgtag cgcgccgaac
     3841 gggaagcctt tttcaggaga gtttgaaatc ggagctgata tcaaaaacga ccatggtcat
     3901 gtcatcttcg gcacttctat cctgcgcaaa ttcgcccaat cgagagagaa gcaaggtggg
     3961 gagtctcgaa acgggctcat tctgcccttc gattaaaacc gaatatagat tctcttctcc
     4021 gaacaaaacg ccgtcggatg tctgatattc cgggattccg tccgtgtaga ccatcaatcg
     4081 atcgcctttg cgcagttgaa gcgttgcgtc ttccagaacc agctggcgca tgattcccat
     4141 cattccgcct ttccccgcca cttcctgaac ctggccgttg cgtaccagta gaggatgagg
     4201 atggccagcg cgcgcaaaac gaagagtaag gctgcgaaga tcgagtacgc cgtagaaagc
     4261 agtgagaaag aatccagtga aagatcgatc gaagaaaatt tcgttcatgg aagaaagaaa
     4321 cgcagcggga ttgcttctat ccgcagactc catggacaca cggaagatcg cagccagaaa
     4381 agcactcgat actccgtgcc cgctcacatc tacaacgaat atctccagcg aatcaccgtt
     4441 gcggcgaatg tcgtatagat ctcctcccac tttgt
//
LOCUS       DAOQJL010000311         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11678_length_4464_cov_4.769562, whole genome shotgun sequence.
ACCESSION   DAOQJL010000311 DAOQJL010000000
VERSION     DAOQJL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11678_length_4464_cov_4.769562"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLG78_13025"
     CDS             <1..732
                     /locus_tag="PLG78_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNE24090.1"
                     /translation="SSFDELADVLAGPQDSPIFKKFLERITTHETRFFRDESTFAALV
                     EQMIPEWMERNRSFGGASRPLNIWCAAGSTGQEAYSIAMLIHSKFPQIFPSLNLVSTD
                     ISETCVERASAGFFTDYEMNRGVSDELRAKFFVRVSDKGYNISSEIRSKVEFKQQNLL
                     VTPYAGKFDIVFCRNVLIYFDLAVRKQILEGFQRVILPDGFLVLGATESPTGLLDSYV
                     VRQFGLTRYYEINSTNITFAPAQMR"
     gene            736..1092
                     /locus_tag="PLG78_13030"
     CDS             736..1092
                     /locus_tag="PLG78_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24091.1"
                     /translation="MKKVLAVDDSQAMLKMVQQTLQIGGYEVLVASDGKEALEKFRGA
                     GVELLITDINMPVMDGITLIKEIRAADKEVPILTLTTESEEPMKQSGFAAGANGWIVK
                     PFRPAQFLDIVRQILS"
     gene            1092..2276
                     /locus_tag="PLG78_13035"
     CDS             1092..2276
                     /locus_tag="PLG78_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24092.1"
                     /translation="MTTPVVLFAGPGLDALAASIRENQYTVLTAESGPQALDLLGAVR
                     VDFFFVAAGSSAAPYVSVLRKKSEAKLILVGNDTSSVPCDRVSSAHAVEEALALLHRL
                     TADRANKELMEQAEGRKVDAEQRRLEWLTWKQQMADRLHMQYSAALVRNIRHSMTQGL
                     GAGALITQVDILPLLPRNPSGDYIVPESSVTFLGEAAEALRHWLSSMEMFVEAMERMY
                     PAETIPEADLSILVSEATQRVDHLRRIKRQTVRSALPTPAGPIRMSRIGLSLAIRELL
                     TNAFKYSPDASRVDIAGFSSQGGYSICILNDLVDLPAGITGIPPERENEIFEPFRRLH
                     NVYDDRFAMEEFPMGTGLTVVRQAIQQAGGSVSLFEIRDHTREDTKKRRVMAEILLPL
                     DI"
     gene            complement(2256..2588)
                     /locus_tag="PLG78_13040"
     CDS             complement(2256..2588)
                     /locus_tag="PLG78_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24093.1"
                     /translation="MEILDFTVLSRYSPNALTTMFGVFESDTVTGLPTITHLMQSGAN
                     PTELARLCHRTRSGCLVMGASRLASVLFDIEKSPGSNEKSPNAEELEDAAKETIAALR
                     EYLDIQGK"
     gene            complement(2575..3297)
                     /locus_tag="PLG78_13045"
     CDS             complement(2575..3297)
                     /locus_tag="PLG78_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24094.1"
                     /translation="MRAICSCALHETSICSLCFEVTDTGIGIEEDRLDKIFNPFAQAD
                     SSTTRRYGGSGLGLSISRELAVMMGGTLTARANHPQGTTFRFESKVIIASPAGRQPLK
                     HPDHQRIENGPRILVADDHELNRMCISLMLRERGLACDLATNGQEALEAARTGQYKII
                     FMDCQMPGLDGYAATQRIRQENLNYSPAIIALTANALEGEKQRCFEAGMDDYIAKPID
                     FERMFELIGRYSGEPEDKNGNS"
     gene            complement(3298..>4464)
                     /locus_tag="PLG78_13050"
     CDS             complement(3298..>4464)
                     /locus_tag="PLG78_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE24095.1"
                     /translation="AKLGGWRLDRKSGLIICTETLLELLDLSPRARLTWPVIKSMISR
                     GSAQVLIGAVHRAFKRGAGFDVQFQIETATGKWIWVRCAGEARRRFSDITGLSGILQD
                     VSASVRSDIALDMERKRLELAMQISRQALFEIDIPSGDIFWDARAFEIHGQNPSAFTP
                     NLRTLDSLLIPEDQGLTDRVQAFFESERRIWHEQYRIKTPDDGIHYIETLGTILRTDR
                     PRIIGIARDITARREIQLELVQAREAAEAATRAKSRFLANMSHDIRTPMTGILGFIKL
                     LQNSGLNQDQQSYADMAASAAGSLRTILDDILDFSRIESGMLRLEKIPVNVADLLSEC
                     TGLFRLSAMEKGVELVYDLAPETPAFILGDPVRLKQILNNLIGNAVKFTEKGRI"
BASE COUNT         1035 a         1099 c         1156 g         1174 t
ORIGIN      
        1 tcttcctttg atgagctggc cgatgttctg gcgggaccgc aggattctcc tatcttcaag
       61 aagttcctgg aacgaattac tactcacgaa acacgctttt ttcgggacga aagcaccttt
      121 gcggcgttag tcgaacaaat gattccggaa tggatggaaa gaaatagatc gttcggtggc
      181 gcttcgcgtc ctctcaatat ctggtgcgcg gccggatcta ccggtcagga agcatactcc
      241 attgcaatgc tgattcattc taagtttcca caaatatttc cgtctttgaa tcttgtctcc
      301 acagacatta gcgaaacatg cgtggaacgg gcgtcggctg gatttttcac ggactatgag
      361 atgaaccgcg gagtgtcaga tgaattgcgc gcaaaattct ttgtgcgtgt ttctgacaag
      421 ggatacaata tctcgagtga gattcgatcg aaggtagagt tcaagcagca aaatcttctg
      481 gttacaccct atgctggcaa gtttgacatt gttttttgta gaaatgttct gatctacttt
      541 gatctggcgg tgcgaaagca aattctagaa ggattccagc gagtgattct cccggatgga
      601 tttctggttc tcggggcgac ggaatcgcca acgggcttgc tggattccta cgtagtacgt
      661 cagttcggcc tcacccgcta ttatgaaatc aattcaacaa acattacttt tgctcctgct
      721 caaatgaggt gatttatgaa aaaggtacta gctgtagatg actctcaggc catgctaaag
      781 atggtgcaac agaccttgca gatcggcggc tacgaagtgc tggtggcatc cgatgggaag
      841 gaagcgctgg aaaaatttcg tggtgccggt gtggagcttc taattacgga catcaacatg
      901 cccgtgatgg acggcattac actgataaaa gaaatcaggg ctgcggacaa ggaagtgcca
      961 atactgacct tgacgactga atctgaagag ccaatgaagc agtctggatt tgcggcgggc
     1021 gccaacgggt ggattgtgaa gccttttcgg cctgcgcagt ttctcgatat tgtccgccag
     1081 attctgagtt aatgactact cccgtcgtct tgtttgcagg accgggcctg gatgcgctgg
     1141 cagcttcgat tcgtgagaac cagtacacag tactaacggc agaatctggc ccacaagcat
     1201 tggatttgct cggagcagtt cgcgttgatt ttttcttcgt ggctgcaggc tcgagcgccg
     1261 cgccatatgt gagcgttctg cgaaagaagt cggaagcaaa actcatcctc gtggggaatg
     1321 acacgtcctc cgtgccttgc gatcgcgttt cttctgcgca tgcggtggaa gaggctctcg
     1381 ctcttctaca caggcttacc gcagacagag caaataaaga actcatggag caggcggagg
     1441 gcaggaaggt cgatgccgag caacgtcgtc tggaatggct tacgtggaag cagcagatgg
     1501 cggatcgatt gcatatgcaa tatagcgccg ctcttgtgcg aaacattcgt cattctatga
     1561 cccagggtct tggtgcaggt gctttgatca ctcaggtgga cattcttcca cttcttcctc
     1621 gcaatccttc tggcgattac attgtaccag aatcttccgt tacttttcta ggagaagcgg
     1681 cggaggccct tcgtcactgg ctttcttcta tggaaatgtt tgtcgaagca atggaaagaa
     1741 tgtatccagc tgaaacaatt cctgaagcgg atttatctat tctcgtatcc gaagcgacgc
     1801 aaagagtgga tcaccttcgc aggattaaac gacaaaccgt tcgctctgca ttgcccacac
     1861 ccgcgggtcc cattcgtatg tccaggattg gtctttcgct cgcgatccgt gaactgttga
     1921 cgaacgcatt taagtattct ccagacgcat cgcgcgtaga cattgcgggt ttctcatcgc
     1981 aaggagggta ttcaatttgt attctcaatg atctcgtgga cttaccggcc ggaattacag
     2041 gcattccacc tgaaagggaa aatgagattt ttgaaccatt tagacgattg cacaatgtgt
     2101 acgatgatcg ctttgcaatg gaagagtttc ctatgggaac agggttgact gtggtacggc
     2161 aggcaatcca gcaggcaggt gggagtgtat cgctgtttga aattagagat cacacgcggg
     2221 aagacactaa gaaaagacgc gtaatggcgg agattttact tcccctggat atctagatat
     2281 tctcgcagcg ccgcgattgt ttcttttgct gcatcttcca gttcctcagc gttaggcgat
     2341 ttttcattgg atcccggaga tttttcaatg tcaaacagta cagatgctaa tcttgatgcc
     2401 cccatcacaa ggcaaccgct tcgcgtacga tgacacagac gagcgagttc agtcgggttc
     2461 gcaccagatt gcatgagatg cgtgattgtg ggtagccccg tcacagtatc gctttcaaat
     2521 actccgaaca tcgtcgtcag agcgttgggc gaataacgtg acagaacagt aaaatcaaga
     2581 atttccattt ttatcttctg gttctccgga gtatcttccg atcaattcga acattcgttc
     2641 gaaatcgatt ggctttgcaa tgtagtcatc catacccgct tcaaaacaac gctgcttttc
     2701 tccttcgagc gcattggctg tcagagcaat gatggcgggg gagtagttta gattttcctg
     2761 gcgaatgcgc tgcgtggctg cgtatccatc cagtcctggc atctggcagt ccatgaatat
     2821 gatcttatac tgacccgtgc gggccgcttc tagagcctcc tgtccgtttg tcgcaagatc
     2881 gcacgccagt ccgcgctctc tcagcatcag cgagatgcac atgcgattga gctcgtgatc
     2941 gtccgcgact agaatgcggg ggccgttttc aatgcgctga tggtcgggat gtttgagggg
     3001 ctgtcttcca gcaggtgacg cgatgattac ttttgattca aagcgaaacg ttgtgccctg
     3061 cggatgattt gcgcgtgcag tgagagttcc tcccatcatg actgcgagtt ctctggaaat
     3121 ggataggcca agcccgctgc ccccgtagcg cctggtggtg gacgagtccg cctgtgcgaa
     3181 tgggttgaag attttgtcca gacggtcttc ctctattcct attcctgtat ccgttacttc
     3241 aaagcacagg ctgcaaattg aagtttcgtg taaagcgcag gagcaaatcg cgcgcactta
     3301 tattctgccc ttctccgtaa acttgaccgc attgccaatc aggttgttta atatctgctt
     3361 gagtcttaca ggatctccca gtatgaacgc cggcgtttca ggtgcgagat cgtaaacgag
     3421 ttcaacgcct ttttccatcg ctgagagtct gaatagtcca gtgcattcgc tcaaaagatc
     3481 cgcaacattc actggaatct tttccaggcg cagcattcca ctttcgattc gcgaaaaatc
     3541 caggatgtcg tccagaatgg ttcggaggga tccggccgca gatgcggcca tgtcggcgta
     3601 actttgctgg tcctgattga gtcccgagtt ctgtaatagt ttaataaatc caagtattcc
     3661 agtcatggga gtccggatat catgagacat gttggccaga aatctgctct tcgctcgtgt
     3721 agctgcttcc gcggcttccc ttgcctgaac cagttccagc tgaatttccc ggcgcgcagt
     3781 gatgtcgcga gcaatcccaa tgatcctcgg acggtccgtt cgcagaatgg ttccaagcgt
     3841 ttctatgtag tgaatcccat catctggcgt tttgattctg tactgctcgt gccagattcg
     3901 tctttcgctt tcaaagaatg cttgcacccg atcagtgagc ccctggtcct caggaatgag
     3961 aagtgaatcc aatgtgcgca ggttcggagt gaaggcagac ggattttgcc cgtgtatttc
     4021 aaacgctctg gcatcccaga agatgtctcc cgagggaatg tcgatttcaa agagcgcctg
     4081 acgggagatt tgcatagcca gctccaatcg tttgcgttcc atatccaggg caatgtcgct
     4141 gcgaacgcta gcgctcacat cctgcagaat tccagaaaga ccagtgatgt cagagaagcg
     4201 gcgcctcgct tcgccggcgc atcgaaccca gatccactta cccgttgcag tctcgatctg
     4261 gaattgaaca tcgaaccctg ctccccgttt gaatgctcgg tgtaccgcac caatcaatac
     4321 ttgtgcagac ccacgagaaa tcatcgattt gatgacgggc cacgtcaggc gagcgcgagg
     4381 cgaaagatca agcaactcca ggagtgtttc tgtgcagatg atgagcccgc tctttctatc
     4441 caacctccac ccgccgagct ttgc
//
LOCUS       DAOQJL010000312         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11760_length_4446_cov_4.749943, whole genome shotgun sequence.
ACCESSION   DAOQJL010000312 DAOQJL010000000
VERSION     DAOQJL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11760_length_4446_cov_4.749943"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLG78_13055"
     CDS             <1..280
                     /locus_tag="PLG78_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24096.1"
                     /translation="AVGSTYTYCIDFNRGTGSSGSYTGFGNMLAVWSRACDQVASSER
                     DQMADVKLMQMMNIPTMNPNNSGKVGFSSNGISLKSFKIGNTIYSSMM"
     gene            288..896
                     /locus_tag="PLG78_13060"
     CDS             288..896
                     /locus_tag="PLG78_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE24097.1"
                     /translation="MKPSTPWLAGFRQLRIALFAAAIAGSLTLLPANLQSEIPLPPGG
                     DFTLQSEQGRVSLSDLKGQVVVLYFGYTSCPSVCPVSLSMISSAFRGLTPEEQASVRI
                     VFVSVDPERDSPKIVGNYARAFGKTAIGLTGSPEQIRETAKRYGVYYRKANLDSAMGY
                     SVDHSSVTYVINARGRLLRLLKHGTKPSEIQEAMRDAIRSKE"
     gene            893..2389
                     /locus_tag="PLG78_13065"
     CDS             893..2389
                     /locus_tag="PLG78_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNE24098.1"
                     /translation="MKHYIFFTICAILPLSLFAQNASFQNPYVQLVPEGMKNSAGFVV
                     VKNTLDTPLKIVRASSPRAASVELHTHTMENGVMRMRPVQVIEVPAKGETSFDPNGYH
                     FMLIGLNGKLVEGEKIPITVEFESGESQTIDFTVKPMATPAMDHSQHSGHNHAGHNHN
                     VADMVAPAGVMGAHMHHPGAWMIDYRAMAMQMNGLQNGTRGVLPFEVLYGIYQDPRVQ
                     MPMTGLAPPSPLLPAARIEQNQFRYMSTGTEMSMEMHMISAMYQAGSDTMLMVMVPYQ
                     ISRMKMLANNFQTANMQARGVGDVSLSATFRVWTSDAHSVFLGAGIALPTGSIDEKDW
                     MPQMGKSQVAYAMQPGAGTASFLPQVAYTGREDRLSWGTQFDGTMRAGKNDNNYRAGN
                     RYNGTAWASWRFADWISVSARVLQQKWENYSGQDPSLDPMMDPGNDPRLQGGRRTDVL
                     AGVNLVATGGAFSGLRLLLEFGRPVRQHLNGPQMSTKWTGNFATQFSF"
     gene            complement(2403..4127)
                     /locus_tag="PLG78_13070"
     CDS             complement(2403..4127)
                     /locus_tag="PLG78_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24099.1"
                     /translation="MFQRVFAIGISSHFPEKLNEKIRLSNKFAIFLAISALPYYPMFY
                     QLKIPYTMELLTFCIVGYCATLVFNALKWNAFARVYMISFFNLMLYAYAMMFQVRTGL
                     KLCYFATATFSFSLFRSVEWRLLVVSLIGSAFAFVAVEFGIMPGWISAIDLGDPEHPL
                     NHAIALTALSLTIGSSLLYFAEVRNYEKKLFAASEQKSLFLSTISHEIRTPLNVIIGM
                     TGLLGDRGLSNQEKDSYVGSIQKSSEYLLSLVNDVLDFERIEKGKVSLDLKAANIKDL
                     FATVKNIMTPLAREKNLSLTVSYPEQAGTYYVFDGRRLSQILINFLANAIKFTGSGGV
                     ALDVTVDKEERDFCVLRFRVVDSGSGISREDQARILEPFFQVSGSSSIQPGFGLGLSI
                     ARELLRLYGAEISVQSEPGKGSIFAFSLRLEKATGASDERNREEHRLPGLHVLAADDS
                     ELNLLVLQKMLGLWDVQVTQVVNGRELVSTYAADSAKYDCVLVDSHMPLMGGVEAAHE
                     LKNRNPDLPVYLVSADATMELERAALAAGANGVITKPINVSTLYNVMLQVQKKKGKIP
                     SPLERSMH"
BASE COUNT         1176 a         1150 c         1105 g         1015 t
ORIGIN      
        1 ggccgtgggc tctacgtata cgtactgtat agacttcaac cggggaacag gcagctccgg
       61 atcctacact ggatttggca acatgctggc tgtctggtcc agagcctgtg atcaagtcgc
      121 ttccagcgaa cgagaccaga tggcggatgt gaagctcatg cagatgatga acatcccaac
      181 catgaatccg aataacagcg gtaaggtggg attcagctcg aacggcattt cattgaaatc
      241 gtttaagatc ggcaacacga tctattcgag catgatgtag gcttgagatg aaaccatcaa
      301 ccccctggct ggcgggattc cgccagcttc gcatcgcgct tttcgccgca gcaattgcgg
      361 gttcactcac tctgttgccg gcaaacctgc aatcagagat tcctctgcct cccggcggag
      421 atttcactct gcaatcggag cagggtagag tctccctgtc agatctcaaa ggccaggtgg
      481 tcgtgcttta ttttggatac acatcctgcc ccagcgtctg cccggtttcg ctttcgatga
      541 tctcgtccgc atttcgcggg ctcactccgg aagaacaagc aagcgtaaga attgtatttg
      601 tatccgtaga tccagaaaga gacagcccga aaattgtagg caactacgct cgcgcttttg
      661 gcaaaactgc gattggactg acgggcagcc ccgagcaaat acgagaaact gcgaaacgat
      721 acggcgtcta ctatcgcaaa gcgaatttgg acagcgccat gggctattcc gtagatcatt
      781 catcggtcac ttatgtgatc aacgcaaggg gtcggctgct cagacttcta aaacacggca
      841 caaaaccatc cgaaatccag gaagcaatgc gagatgccat caggagcaaa gaatgaaaca
      901 ctatattttt tttacgatat gcgccatatt gccactgtcc ttgttcgccc agaacgcttc
      961 ctttcagaat ccgtatgtgc agctcgtgcc ggaaggaatg aagaactccg cgggctttgt
     1021 tgtggtgaaa aatacactcg atactccatt aaaaattgtg cgggcatctt cgcccagggc
     1081 cgcctcggtg gaactccaca ctcacaccat ggaaaatgga gttatgcgaa tgagacccgt
     1141 acaggtgata gaagtgccgg caaaaggaga gacttctttt gatccgaatg gatatcattt
     1201 catgttgatt ggtttaaacg gaaagctcgt agaaggagaa aaaattccaa tcacagttga
     1261 gtttgaatcc ggggaaagcc aaacaataga tttcacagtg aaaccaatgg cgacgcctgc
     1321 catggatcac agccagcata gcggccacaa tcatgcaggg cacaaccaca atgtcgcgga
     1381 catggttgca cccgccggag tgatgggcgc acacatgcac catccgggag cgtggatgat
     1441 cgactatcgg gccatggcca tgcaaatgaa cggactccag aatggaacgc gtggcgtgct
     1501 tccattcgaa gtgctgtatg gaatctatca ggatcctcgc gtgcagatgc cgatgacagg
     1561 tcttgcgccg ccatcgcctc tcttacctgc agctcgaatt gaacagaatc agtttcgcta
     1621 catgtctact ggaacagaaa tgtccatgga gatgcacatg atctccgcga tgtaccaggc
     1681 gggctcagat acaatgttga tggttatggt gccttaccag atcagcagaa tgaaaatgct
     1741 cgcgaacaat tttcaaacag caaacatgca agctcgcggt gtcggcgatg tgagcctttc
     1801 tgctactttt cgcgtatgga cgagtgacgc tcactccgtg tttctgggag caggcattgc
     1861 acttcccacc ggttccatag atgaaaagga ttggatgccc cagatgggaa aatcgcaggt
     1921 cgcatatgcc atgcaacctg gagccggcac agcttctttt cttccgcaag tggcttacac
     1981 tggcagagaa gatcggttga gctggggcac tcagtttgac ggcaccatgc gagctggaaa
     2041 gaacgataac aactatcgcg caggcaatcg ctacaacgga actgcatggg catcgtggcg
     2101 atttgcggac tggatcagcg tgtccgctag agtcttgcag caaaaatggg aaaactattc
     2161 cggtcaggat ccttctcttg accccatgat ggatcctgga aacgatcctc gccttcaggg
     2221 tggaaggcga acggacgtac ttgctggagt caatctggtt gccactggag gtgcattttc
     2281 cggactcaga cttctgctgg aatttggaag accagtcagg caacacctga acggacccca
     2341 gatgtctacg aagtggacag gaaatttcgc gacacagttt tccttttgaa ggttcgaatc
     2401 tcctaatgca tgctccgttc aagaggtgac gggatctttc ccttcttctt ttgaacctgg
     2461 agcatcacgt tgtagagcgt actaacgtta atcggctttg tgatcacccc gttcgccccg
     2521 gcagcgagtg ctgctcgctc cagttccata gttgcgtccg cagataccag ataaacaggt
     2581 agatcagggt ttcgattctt cagctcatga gctgcttcta ctccgcccat caaaggcatg
     2641 tgactatcca cgagcacgca atcgtacttt gcgctatccg cggcgtaagt ggaaaccagc
     2701 tcacgaccat ttacaacttg cgtcacttga acatcccaca gccccagcat tttctgcaaa
     2761 acaagaagat tgagttctga atcgtccgcc gcgagcacat gcaagcccgg cagccgatgc
     2821 tcctcccgat tcctttcatc actggcgccg gttgccttct caaggcgcaa agagaacgca
     2881 aaaatgctac ctttgcccgg ctcactttga acgctgatct ccgcgccata caatcgcaat
     2941 aattctcgcg cgatggaaag gcctaaaccg aaccccggtt gaatggaaga gctgccggac
     3001 acctgaaaaa acggctcaag aatgcgcgcc tgatcttctc ttgaaattcc agacccacta
     3061 tccaccacgc gaaacctgag cacgcagaag tcgcgctctt ctttgtctac agtcacatcc
     3121 agggccacgc cgccgctgcc agtgaacttg atcgcattgg caaggaagtt gattagaatc
     3181 tgactcaggc gacgaccgtc aaacacatag taggtgcccg cctgttccgg atatgagacg
     3241 gtcaaactca gattcttctc acgcgccaga ggtgtcatga tattcttgac tgtcgcaaac
     3301 aagtccttta tgttcgcagc cttgagatca agagatactt ttcctttctc aattctttca
     3361 aaatcaagga catcatttac aagggagaga aggtactccg aacttttttg aatggaaccg
     3421 acatagctat ccttctcctg attggaaagt cctcgatcac caagcaagcc ggtcatccca
     3481 atgatgacgt tcagaggagt gcgaatctca tgcgaaatag tagaaaggaa aagagatttt
     3541 tgttccgaag cggcaaaaag cttcttttcg tagttacgaa cttcggcaaa atacaagaga
     3601 gatgaaccga tcgtgaggct cagtgcagtc agggcaatcg cgtgattcaa cggatgctcc
     3661 ggatccccaa ggtcaatcgc agagatccag cccggcatga tgccgaattc caccgccaca
     3721 aacgcaaacg cgcttccgat gagagacacg acaagcaatc tccattcgac acttctaaag
     3781 agggaaaaag aaaacgttgc cgtcgcgaaa taacagagct tcaaccctgt gcgaacctga
     3841 aacatcatcg cgtatgcgta gagcatcaaa ttgaaaaagg atatcatgta gacccgggca
     3901 aaagcgttcc acttgagagc gttaaataca agcgtcgcgc agtagcctac gatgcagaaa
     3961 gtaagaagct ccatcgtgta cgggatctta agctgataga acatggggta gtacggcagt
     4021 gccgaaatcg ccagaaaaat agcgaacttg ttgctaagac gaatcttctc gtttagcttt
     4081 tcagggaaat gcgaagatat gcctatggcg aagacgcgct ggaacataca cactccctat
     4141 attgcggcag gcgcgaatca atcgcggtgg atgaaagcta ctgtacactc tcgttttact
     4201 ccacagccag cggtccttgt cgagtgaatt tggataaatc gcgaacgttc gaagatgaac
     4261 ggcaccgaga ttgagtcgca tttatcgcaa tggatcaacc aatggcgcat aacgtagatt
     4321 tgtcaaatat cgtcgagcaa atcatcgtcc gcagaatttc gtttcttctt caccttttct
     4381 ctatgcgcgt gagcgcgaac aggtttcgcc ggcggcacgg atgctgtcac tttccccgcg
     4441 tcacgg
//
LOCUS       DAOQJL010000313         4430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11817_length_4430_cov_5.046171, whole genome shotgun sequence.
ACCESSION   DAOQJL010000313 DAOQJL010000000
VERSION     DAOQJL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11817_length_4430_cov_5.046171"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="modA"
                     /locus_tag="PLG78_13075"
     CDS             <1..773
                     /gene="modA"
                     /locus_tag="PLG78_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE24100.1"
                     /translation="SKLSLMLDPAHFRTRNLSFILKQPARNRVCVLIWVLSCVFISCS
                     PTGPRPVTIFAAASLKETISELSNEFEKSGRKTQVQFDASSTLARQIQQGALADVFIT
                     AAPEWLESMRILDRFDWLSNRLVIVVPLNDKSSFRLRNMKSLALANEQVPAGKYARAA
                     FAHLHIPIPERTIYGSSVRDVLFIVAKGGAEAGVVYATDAATAPELGVAFTFPEESHP
                     RIVYSVGLLRPEGKEFYDLLQARPEAATKHGFIDIRKQ"
     gene            770..1441
                     /gene="modB"
                     /locus_tag="PLG78_13080"
     CDS             770..1441
                     /gene="modB"
                     /locus_tag="PLG78_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNE24101.1"
                     /translation="MNSIQLSLSVGFAATLLTLPLAILAAWFLEKTDSKARPILQILI
                     TLPLVLPPVVIGYLLLALFSPSGKVGSTLQWLGAPVAFHWLGAVVSAAVVGFPLFVTS
                     ISLSMREIDARLERISASLGASRLQTFFRITLPLAWPGILSGAALAFARGLGEFGATI
                     ILAGRIPGQTETIPLAIYAELDSPGGETKIAWLVFASIAICVILILVSRIFQGLHKRK
                     MESES"
     gene            1438..2436
                     /locus_tag="PLG78_13085"
     CDS             1438..2436
                     /locus_tag="PLG78_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE24102.1"
                     /translation="MILEIEANVSRPNGFHLIVEEKWSAHATGIAGPSGGGKTTLLNC
                     ITGSERGARVIVDGEDWSEWSMQERKIGYVTQDPLLFPHMTVKENLLYSPRASSIGPI
                     AEMLRIDHLLERRPANLSGGEKRRVALGRAIASQPRLLVLDEPFTGLDEILRREAMSV
                     LHSIKQWSGVPMILVSHLADEIVGLTDWTIRLENGSVSKRGPSESVLNETETRIDNYF
                     SGIILEPGLVRCGPCDLKVILPHDADGPVRLACYANDILLSKKPPDEISARNVIPTKI
                     KLIHGFEKITLVDLEAPSLRVLVTREANRDLDLVTGMSIYAIIKASSIVYLGPAEN"
     gene            complement(2433..2819)
                     /locus_tag="PLG78_13090"
     CDS             complement(2433..2819)
                     /locus_tag="PLG78_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5360 family protein"
                     /protein_id="HNE24103.1"
                     /translation="MRFLPFFFLVTDIGFILYWVITLIGIIPADYLFKDYNNPILSAW
                     NWSFLPLDLFISFSGLYSLRLRAKGFPWKNVALISLVLTLCSGLQAVSFWAIRRDFDL
                     AWWLPNLFLFLYPIYFIAAILRGSRD"
     gene            2914..3420
                     /locus_tag="PLG78_13095"
     CDS             2914..3420
                     /locus_tag="PLG78_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE24104.1"
                     /translation="MKYLLFAFLLISASLLAEEEKLATLETACQEGEVEKVKSILKGI
                     PGAEQNQKAKGCLATAVSEAHIPLVRFLVERKTPLDHHYGWTPLHRAAKLSTPDHLKI
                     ARILVDAGARVDVGLKSPDGEPYMGPYGIGMSPLGVARKSRDKFTDEKARAPFDAMIA
                     FLKSRSGQ"
     gene            complement(3499..3924)
                     /locus_tag="PLG78_13100"
     CDS             complement(3499..3924)
                     /locus_tag="PLG78_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24105.1"
                     /translation="MKRLSTIFTIFIFFVGAVLPGCKKEEKDHTSEYALIVLFAASFT
                     RTFSFSCNKFSVNSSCTNYYGFGTSSATCTSGGGTVSTSKCSNLSNYGTCTTAAGGSG
                     SSEFVFYSGGTNPVCGSAGACQTNCTAVGSGTYSSTYTP"
     gene            4047..4289
                     /locus_tag="PLG78_13105"
     CDS             4047..4289
                     /locus_tag="PLG78_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNE24106.1"
                     /translation="MGKTVTVRLDDKTYRMIRSAAEADHRTISNFMEVATLSHILEDN
                     FASDEEMGEILANKPLVKRLKASAAAAKKGKFRIVG"
BASE COUNT         1200 a         1111 c         1104 g         1015 t
ORIGIN      
        1 actcaaagct ttccctgatg ctagatccag cccatttcag aacgcgcaat ctatcgttca
       61 ttctcaagca acctgcacgc aatcgtgtct gtgtccttat ctgggtcctc tcgtgcgttt
      121 tcatttcttg cagtcctact ggtccgcggc cagtcactat ttttgcagcg gccagcctga
      181 aagaaaccat cagcgaactt tccaatgaat ttgagaagag tggaagaaaa acgcaagttc
      241 aattcgatgc atcctcaacc cttgcgcgac agattcaaca gggagcgctc gcggatgtat
      301 tcatcactgc cgctccagaa tggctcgaaa gcatgaggat tctggatcga tttgactggc
      361 tttccaatcg tcttgtaatt gttgttccct tgaatgacaa atcctctttc cgcctccgca
      421 acatgaagag tctggctctt gccaacgaac aggtcccggc aggaaagtac gcgcgcgcag
      481 cgtttgctca ccttcacata ccgattccag aaaggaccat ctacggttca agcgtgcgcg
      541 atgtgctttt tattgttgca aaaggtggcg cggaagcagg cgtcgtgtat gcaacggacg
      601 ctgcaaccgc accagagctt ggcgtggcct tcacgttccc tgaagaaagt catcctcgca
      661 tcgtgtactc cgtcggtttg ctgcggccgg aaggaaaaga attctacgac ctactgcaag
      721 cgcgcccgga agcggctaca aaacacggat tcatagacat tcgaaaacaa tgaacagcat
      781 ccaactatca ctctccgtcg gattcgcggc aaccctcctt acacttcctc ttgccatctt
      841 agctgcgtgg tttctggaaa agacggattc aaaagccaga cccattttgc agattctaat
      901 caccctacca cttgtgctcc ctcccgtcgt gattggctat ctgctactgg ctttgttttc
      961 tccctcggga aaagtcggct ccacattaca gtggctaggt gctccggtag cgtttcactg
     1021 gttgggcgca gttgtttccg cagccgtagt cgggtttccc ttatttgtaa cgtccatctc
     1081 tctttccatg agagaaatcg atgcgcgtct ggagcggatt tctgcatctc tgggagcttc
     1141 acgcctgcaa actttcttca gaatcacgct tcctctcgcc tggccaggaa tactatcagg
     1201 cgccgcactt gcattcgcac gcggactcgg agaatttgga gcaaccatca tcctggcggg
     1261 tagaatcccc gggcagacag aaaccattcc gcttgccatc tatgctgaac tggattcgcc
     1321 gggcggagaa actaaaatcg cctggcttgt cttcgcatcc attgcgatct gcgtaatctt
     1381 gattcttgta tctagaatct tccagggcct gcacaaaagg aaaatggaat cagaatcatg
     1441 attctggaaa tcgaagcgaa tgtctctcgc ccaaatggtt ttcatttaat cgtagaagaa
     1501 aagtggagcg ctcatgcaac cggcattgcg gggccgtcag gcggcggaaa gaccactctg
     1561 cttaactgca tcaccgggag cgaaagaggg gccagagtca tagtggatgg agaagactgg
     1621 tccgaatggt ccatgcagga gcgcaagatt ggatatgtta cgcaggatcc tcttttattt
     1681 ccacacatga ctgtgaaaga gaatctactc tatagcccgc gcgcaagttc cattggtccg
     1741 attgcagaaa tgctgcgcat agatcatctt ctggaacgca ggccagcaaa tctgagcgga
     1801 ggagaaaaac gacgtgtggc cctggggcga gcgatcgcca gccagccccg cttgcttgtt
     1861 ctagatgagc cattcacggg actcgacgaa attctacgac gcgaagcaat gtcagtacta
     1921 catagcatca aacaatggtc cggcgttccc atgattctag taagccatct cgctgatgag
     1981 attgtagggc tgacggactg gacgattcgt ttggagaatg gttccgtctc gaagcgcggt
     2041 ccgtccgaat ccgtgctgaa tgaaacagaa accagaatag acaattactt ttccgggatt
     2101 attttagagc cgggtctggt tcgctgcggt ccatgcgatc taaaagtgat tctcccacat
     2161 gacgcggatg gtccggtcag gctcgcctgt tacgcaaacg acattcttct ttctaagaaa
     2221 ccgcctgacg aaatatcagc gcgaaatgtg attccaacca aaatcaaatt gattcatggc
     2281 tttgaaaaaa tcacgttagt cgaccttgag gcgccgtcgt tgcgagttct ggtgaccaga
     2341 gaagcaaatc gagatctgga tctggttact ggaatgagca tttacgcaat catcaaggca
     2401 agttccattg tctatctggg tccggcagag aactagtccc ggctgccacg caagattgcg
     2461 gcaatgaagt agatcgggta gagaaacaaa aacagattcg gtagccacca ggccagatcg
     2521 aaatcgcgac gaatcgccca gaaagacact gcctgcaggc cggagcacaa cgtcagcacc
     2581 agagatatga gtgccacgtt cttccaggga aacccctttg cgcgaaggcg aagactgtag
     2641 agcccggaaa aagaaatgaa cagatccaga ggaagaaacg accaattcca ggcgctgagg
     2701 attggattgt tataatcctt gaataaataa tccgcaggaa tgattccgat cagagtgatc
     2761 acccagtaga gaatgaaccc aatgtctgtg accaggaaga aaaagggaag gaagcgcatg
     2821 attgtttcaa gttggagaaa gacggcgcgg ggcacaagcc caaagaagag tggatggaag
     2881 ttgacatttc ctgcagtgcg gaaaggattc ggcatgaaat atttgttatt cgcgtttcta
     2941 ctcatctctg cctctcttct tgcggaagag gaaaaactgg ctaccctgga aacagcgtgc
     3001 caggagggag aggtcgaaaa ggtaaaatcc attttgaaag gaattcccgg ggcggaacag
     3061 aaccaaaaag cgaaaggatg tcttgctacc gcagtgtcag aagcgcacat tcctctggtt
     3121 cgtttcctcg tggagagaaa gacacctctg gaccaccact acggatggac tcctctgcat
     3181 cgcgcggcaa agctctcaac accggatcat ttgaagatcg cacgtatcct ggtggatgcg
     3241 ggtgcacgag tggacgtcgg actcaagagt ccggacggag aaccttacat gggcccatat
     3301 ggaattggaa tgtcaccact cggtgttgcg cgcaaaagca gagacaagtt tacagacgag
     3361 aaagcacgtg caccgtttga tgcgatgatt gcgtttctca aatcgcgcag cgggcagtga
     3421 tagaatttcg cgcatcagca aattcttcca aaccacggcg cgaaattctt cagcggacag
     3481 ataccaaacg cgaagcgatc aaggagtgta agttgaagag tatgtaccgg aaccgacggc
     3541 tgtgcagttt gtctgacagg cgcctgcgct accgcataca gggttcgtgc cgccggaata
     3601 gaacacaaat tcagaggagc cagaaccgcc agcggcagtc gtgcacgttc cgtagttcga
     3661 tagattcgaa catttggaag tggaaactgt gcctccaccg gaggtacacg ttgcagaact
     3721 cgtgccaaat ccgtaatagt tcgtgcaact actgttcacg ctgaatttat tgcagctgaa
     3781 tgaaaaagta cgagtgaagc ttgccgcaaa caatacgatc aacgcgtatt cagatgtatg
     3841 atctttctcc tcctttttgc agcctggtag aacggctcca acaaaaaaga tgaaaatagt
     3901 aaaaatagta gacaatctct tcatggcctt ctgtaccccc cccccccgaa ttttcaacta
     3961 tttatccgag aatatccaat ggatttgatc cgaatagtgt tgacttttct gaatacaaat
     4021 gaatgcatta gaacaggatc gaatcaatgg gaaaaacagt taccgtacgc ctcgatgata
     4081 aaacatatcg catgatcaga tccgcggcag aagcagatca cagaaccatc tccaatttca
     4141 tggaagtggc cacattatcc cacatcctgg aagacaactt cgcctctgac gaggaaatgg
     4201 gggagattct ggcaaacaaa ccgctggtga agcggttgaa agcttccgca gctgcggcga
     4261 aaaaaggcaa gttccgaatt gtcggctgaa ttcaggatcg cagaaacgga gttcgccaga
     4321 acagccctcg catctccgaa acacaaagcg cttgcaaaaa aactcaatgc attcgtttac
     4381 ccacagcttc gcaaaaatcc tttctacggt ccgaatatta agaaattgaa
//
LOCUS       DAOQJL010000314         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11885_length_4414_cov_4.560450, whole genome shotgun sequence.
ACCESSION   DAOQJL010000314 DAOQJL010000000
VERSION     DAOQJL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11885_length_4414_cov_4.560450"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..71
                     /locus_tag="PLG78_13110"
     tRNA            1..71
                     /locus_tag="PLG78_13110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32..34,aa:Ile,seq:gat)
     gene            complement(59..3391)
                     /locus_tag="PLG78_13115"
     CDS             complement(59..3391)
                     /locus_tag="PLG78_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24107.1"
                     /translation="MAKKTQKKPAKKIEKPAKKPAKAPEKKPLPVKKALPVPAKKPTA
                     VKPAPPTRPAAREKGDFVSTWIARGQTFESVEQLFHEAAIFLRKNFGFTSLNLFSGSG
                     SSPLLAVHHDGTSYVSENLDTTGHAPDAGTITKESKVFIIAENKPSSVSIQGSIASAS
                     VNAPGHQSGILVAASEDPAPPYLKSDLALLQSFAQFLGPVLSHFQLMAVETKNRDLSA
                     ELKETQKNARESQKTLTADSARKIEELTAEHESRLSAQKESLLAEAEEKTRALAEKLE
                     ARIEESSQTALRLREELQTAHRERDEAAATARKESQDRIAELELELDDLKSRAAEQKA
                     AAIQEIETAAAAEKADLRAKLDAAEREKQSALSRTSALEANLEKIQKEAHDLRAEIEA
                     RDALLSETGSEHTRKEAQLTSQIEEIKKQHAAEKQKLENEKASILESKAAVEKALSDS
                     HNQSESAVADLHKKIAALELRLREESEKAQKEIAFQEAKNREITSHLEDEKKKLQADK
                     KKHEDELAREKDARAQRERELSEARNAGDRKTGELQTEITKRESQISSLKEEINNLNQ
                     EIHKRNSRIGEMERQIADQKNRIVSLEAEVGKRDNTIKKLEADLELARKESRRISEEK
                     AEAQKQIKSLEELLLVRDRELKNQAEEIALRDEELARLAGQIQSLKENKESQDTRIAG
                     LEKEIAHYRERESRLEKDIVTLQDEKRVLGGKIESQNEEIASLKGGVQKEVRERESVQ
                     AELASVRTREKKMSEELQSALNREKGSREEGVILFNFARALSETESFKGKLERLASGL
                     SARLNISRIFAYSLKGEDLLLLEEALHGKDWLNQSKKTHIRVSETVMGNALASLKPDA
                     LQPGVSEIPEKLLHDILGSSNGSAGKGNGIVLPLTESLETLGALCIFADGPVGESDVR
                     LLANLAPLMATALRMRLEERSRRSGKEGFERKNAIIHFLEKKLHKQGIEIDPARKPEE
                     QLAAFEVFAHNLGRICHEEGIVTNLEIEPASLLALSDRITSPTHLHYILLEAVENVRA
                     HAEATRLAIRLRSVGEDIALVIEDNGEGLLRKAGTENPAKGTGIAAIKNLAMDAGASV
                     SMSKGAEGRGLRIECVWHSGPG"
     gene            complement(3395..4216)
                     /locus_tag="PLG78_13120"
     CDS             complement(3395..4216)
                     /locus_tag="PLG78_13120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE24108.1"
                     /translation="MKNRVYLVTGATSGIGRALALRAAQAGDTVFAGVRELSRAPSSE
                     NIRPIQLDVTNPEDVRRASETILQQCGNIDVLVNNAGFGVYGAFEELSEEQIRAQMET
                     NYFGCLAMARAVLPSMRERRSGTIINVSSILGRITIPTGSAYTASKYAIEAFTESLRQ
                     EVKPFGIQVFAVEPGLIRTSFKQNMVKSHAVQKNDSPYAFLNRMIQKEYSGFSTSQER
                     AADRIYKLSQKKRPARRYRVGLDASFYYTLSRILPDSLLDNLVALGVRREFKKTS"
     gene            4342..4414
                     /locus_tag="PLG78_13125"
     tRNA            4342..4414
                     /locus_tag="PLG78_13125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4375..4377,aa:Ala,seq:tgc)
BASE COUNT          798 a         1117 c         1120 g         1379 t
ORIGIN      
        1 ccagtagctc agttggttag agcacttctc tgataaggaa ggggtcggta gttcgagtct
       61 accctggccc agagtgccaa acgcactcaa tccggagtcc gcgtccctca gcacctttgg
      121 acatactcac acttgctccc gcatccatcg ccaggttctt gatcgccgca atgccggtac
      181 ctttagccgg attttctgtc ccagccttgc gaagcagtcc ttcaccgttg tcttcgatta
      241 caagagcaat gtcctctccc acagaacgaa gccgaatcgc caggcgcgta gcttccgcat
      301 gtgcgcgaac attttccact gcctcaagta ggatgtagtg gagatgcgtt ggcgaggtga
      361 tgcgatcgct caaagccaga agactcgccg gttcaatttc aaggttcgtg acgattccct
      421 cttcgtggca gattcttccc agattgtgtg cgaagacttc aaaagctgcc agttgctctt
      481 ccggtttgcg cgccgggtcg atttctattc cctgcttgtg cagcttcttc tcgaggaaat
      541 gaatgatcgc gttttttctt tcgaatcctt ctttgccgga tctcctggat ctttcttcga
      601 ggcgcatgcg aagagcggtt gccatcagtg gtgcgagatt tgcgagaagt cgcacatcgg
      661 attcaccaac ggggccatct gcaaaaatgc acagggctcc gagagtttcc agagattcag
      721 tgagaggcag aacgattccg tttcctttgc ctgcgctccc gtttgaactt ccgaggatgt
      781 cgtgcagtag tttttccggg atctcgctta cacctggttg cagtgcgtcc ggtttgagac
      841 tggccagtgc attgcccatg accgtttcgc tgacgcggat gtgagtcttt ttggactggt
      901 tgagccagtc ttttccgtgg agcgcttctt ccaggagaag tagatcttct cccttcagac
      961 tgtatgcgaa gatgcgagaa atatttaaac gagcggatag cccggacgcc agcctttcca
     1021 gttttccttt gaaagattct gtttccgata gggcgcgcgc gaagttgaac agaatgacgc
     1081 cctcttcgcg cgatcccttt tcgcgattca atgcggactg aagttcctca gacattttct
     1141 tttcgcgagt gcgcacactg gcaagctctg cctgaacgga ttctctttct ctaacttctt
     1201 tttgcactcc gcctttgagc gaagcaattt cttcgttctg gctttcgatc ttgccgccca
     1261 gaactctttt ctcatcctgc agggtcacaa tgtctttttc cagtctggat tcgcgttctc
     1321 tgtagtgagc gatttctttt tccagtccag caatgcgcgt gtcctggctc tctttgtttt
     1381 ctttcagaga ttgaatctga cctgccaggc gggccagttc ctcatcgcga agtgcgattt
     1441 cttccgcctg attcttcagt tcgcggtcac gcaccagcag caattcttcg agggacttga
     1501 tttgtttctg agcttctgct ttttcttctg aaatgcgtct ggattctttt cttgcaagtt
     1561 ccaggtctgc ttccagtttt tttatggtgt tatctctctt gccgacttca gcctccagag
     1621 aaacaattct gtttttctga tctgcaatct ggcgttccat ttcgccgatt ctgctatttc
     1681 ttttgtgaat ttcctggttg agattgttga tctcttcttt cagagaagag atttgagatt
     1741 cacgtttggt gatttctgtc tgcagttcac ctgtctttct atccccggcg ttgcgcgctt
     1801 cgctcagttc tcgttcgcgc tgagctcttg catctttttc gcgcgcaagt tcatcttcat
     1861 gtttcttctt gtctgcctgc agtttctttt tttcatcttc gagatgactt gtgatttcgc
     1921 ggttctttgc ttcctggaat gcgatttctt tttgagcttt ttctgactct tcacgcagac
     1981 ggagttcgag agctgcgatt ttcttatgca ggtctgccac tgccgattct gattgattgt
     2041 gtgaatcgct cagagctttc tctactgcgg cttttgattc caggatggat gctttttcgt
     2101 tttccagctt ttgtttttct gccgcatgct gctttttgat ttcttctatt tgcgatgtga
     2161 gctgcgcttc cttgcgcgtg tgttcgcttc cagtttcgct tagaagtgcg tcgcgcgctt
     2221 caatttctgc tctgaggtcg tgcgcttctt tctggatttt ttctagattc gcttccagag
     2281 cagacgtgcg cgacagtgca gactgctttt ctctctctgc tgcatcaagt ttcgcacgga
     2341 gatctgcttt ttccgcggcg gctgcagttt cgatttcttg gatggccgct gccttctgtt
     2401 ctgcggctct agatttgaga tcgtcgagtt cgagctctag ttccgcgatt ctgtcctggg
     2461 attccttgcg cgctgtggcg gccgcttcgt ctctttctct gtgagctgtc tgcagttctt
     2521 ctcgcagacg cagtgcggtc tggctgcttt cttcgatgcg ggcctcgagc ttttctgcga
     2581 gtgctctagt cttttcctct gcttctgcta aaagggattc tttttgggcg ctcaggcgcg
     2641 attcatgttc tgccgtgagc tcttcaattt ttcgtgcgga atctgcggtc agagtcttct
     2701 gtgattcgcg ggcattcttt tgcgtttctt tgagctctgc ggacagatcg cggttcttcg
     2761 tctcaacggc catgagctgg aaatggctga gtacgggccc cagaaactgg gcaaaagatt
     2821 gcagcagagc cagatcactt ttgagatagg gtggtgccgg atcttcggaa gcggccacaa
     2881 ggattccgct ctggtggccc ggtgcattca cagaggcaga agcaatggat ccctgaatgg
     2941 aaacagaaga aggtttgttt tctgcaatga tgaatacttt agattcttta gtgatggtcc
     3001 cggcatccgg tgcgtgtccc gttgtgtcca ggttttctga gacgtagctg gttccatcgt
     3061 gatggacggc cagaagcggg gaggatccag atccagagaa tagattgaga gaagtgaatc
     3121 caaagttctt tctcaggaag atagccgctt cgtgaaacag ctgttctacg ctttcaaacg
     3181 tttgtccgcg cgcgatccac gtgcttacaa agtctccctt ttccctggcc gccggccgtg
     3241 tgggaggagc aggtttcaca gccgttggtt tctttgccgg aacaggcaat gctttcttta
     3301 ccggtagggg ctttttttca ggtgcctttg ccggtttctt agctggcttt tcgattttct
     3361 tcgctggttt tttttgggtc ttttttgcca taaatcaaga tgtctttttg aattcgcgcc
     3421 tgactccgag cgcaacgaga ttgtctagta gggaatctgg aagaatgcga gacagtgtgt
     3481 agtagaaaga agcatccagg ccaactctgt atcttctggc cggtcttttt ttctggctca
     3541 gcttgtagat tctatccgcg gctctttcct gtgacgtgga aaaaccgctg tattctttct
     3601 ggatcatgcg gttcaaaaat gcgtaaggcg aatcgttttt ttgaactgcg tgcgatttca
     3661 ccatgttttg cttgaagctc gtgcgaatca atcctggctc cactgcgaat acttgaattc
     3721 caaaaggctt cacttcctgg cggagacttt ctgtgaatgc ttcgatggca tacttggaag
     3781 cagtgtatgc ggagccagtt ggaatcgtaa ttcttccgag gatggacgac acgttgatga
     3841 tggtgccgga tctgcgctca cgcatggaag gaagaacagc tctggccatg gccagacaac
     3901 cgaagtaatt tgtttccatt tgcgcgcgga tttgttcttc gctgagttct tcgaacgcac
     3961 catatacacc aaagcccgca ttgtttacga gcacatctat gttgccgcac tgctggagga
     4021 tcgtttcgga tgcgcggcgc acgtcttctg gattggtaac gtccagctgg atgggcctta
     4081 tgttctcaga cgaaggagcg cgagagagtt ctctgactcc agcaaataca gtgtctccgg
     4141 cctgagctgc gcgtagcgca agtgctcgac cgatccccga cgtggctccc gtcaccagat
     4201 agactctgtt tttcatgcct ggtggaagag ttccacaatc actctcgcag gaaagtcaaa
     4261 aactgtctca gctctaaaat ctgctttaca cgaaaaggcc catttgcatt tcatacattt
     4321 tggccgctcc tgggtcataa tggggactta gctcagctgg gagagcgtct gccttgcacg
     4381 cagaaggtca ccggttcgat cccggtagtc tcca
//
LOCUS       DAOQJL010000315         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_11931_length_4404_cov_4.618763, whole genome shotgun sequence.
ACCESSION   DAOQJL010000315 DAOQJL010000000
VERSION     DAOQJL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_4404_cov_4.618763"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1553
                     /locus_tag="PLG78_13130"
     CDS             72..1553
                     /locus_tag="PLG78_13130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE24109.1"
                     /translation="MIPWNKPAPADVNRQDPENVFAAGTRNHHISNTAYPIAHNVTWK
                     PYRVDRNANASLVESAFADAETISLYSHIPFCETRCYFCEYTVVGKKELDAASDYMKL
                     LSRELAMFRSLLGERSLSGFDIGGGTPSFVNAPLMAEHVDEVRKAFRTAPGFAISIET
                     TPRIASTEPEKMKAYADMGIERISMGIQVLQPDLLKHLGRSENGLDHHRKACDHIRGA
                     GFKKFNIDLMYGFAKQSDQSWEATLRHAIALEPDYITLYRMRYKLTRISAEAADVTLE
                     PVLRQAKLASEMLRSAGYIANPGKNTYSRIPKDTGTSAYLTRRVIEGSTYLGLGLGAQ
                     TFTDTTISYNDGAASKSIAPYAKSIAEGRLPIQDLYHLPLEHMMAKMIAVSFYFGEIR
                     ADAFEKKFKVSLEEAFPREIEFALKEGLMHWSISENGQEIPGSSDRRALSLTPHGARN
                     FNGTIALFFAPSVQSYLIHRQSQADMHRHAASAQLVADKNDAA"
     gene            1550..2314
                     /locus_tag="PLG78_13135"
     CDS             1550..2314
                     /locus_tag="PLG78_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE24110.1"
                     /translation="MSHDFANILFAGPCNRFCPFCIGKELPDRANEENLNLYPLRNQD
                     AFIREVQRLDIREIVMTGTTTDPQLYRHEERLLSEMRRQLPHARFSVHTNGVLALRKL
                     EAFNSYDRACISFPSFRSDTYEKMMGSRIVPDLRSILRESRIPVKVSCVINEHNVHEL
                     DDFILEAHSIGVRRLVLRKLFGETRTWDVLKDRIPESHYRDNPVYNVDGMEVTYWDFD
                     RTTSKSINLFPDGTIGPSYLLAATPAFMARSSAIPG"
     gene            complement(2257..3081)
                     /locus_tag="PLG78_13140"
     CDS             complement(2257..3081)
                     /locus_tag="PLG78_13140"
                     /inference="COORDINATES: protein motif:HMM:NF021998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HNE24111.1"
                     /translation="MSIKKDSKVRATSHECIHMENSAVKPDIIMEEDAFAHSAGPVHV
                     FFEHLATYTYPIVFTSTLALSVLEFNNARNLYWLLLLMPLGWLAADWITGIIHWFFDT
                     YGSQTMPVLGRSFIKPFRDHHIDPKGITTHSLTVTIGNTCIAAVPITFWILYPLLFGD
                     PTDAYRMTAFFLSFTIGGTVLTNQFHKWSHLDTPPLWIRTLQKGKIILGIVHHDVHHT
                     KPFDTFYCITNGWMNPILDRINFWRGMEKLLGWFGVKPYPGIAEERAINAGVAASR"
     gene            3135..4118
                     /locus_tag="PLG78_13145"
     CDS             3135..4118
                     /locus_tag="PLG78_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNE24112.1"
                     /translation="MTHPNQALFSGEKPFPVIPSCEHFAGNEKMIGKALQLQQEKGPV
                     FDITQDCEDGAPQGQEVEHAKMIVRITNSSENKLGMAGVRIHDHSNPHWKQDVDILVP
                     GAGDKLAYITIPKPTAASQVEEMIDYIRNAASKAGLKREIPIHVLVETHGALRDADKI
                     AAMPWMQVLDFGLMDFISGHFGAITLSNMKSPGQFDHRLISRAKANVVAAALANGIIP
                     AHNVTLDLKNFEQTKSDATRARYDFGFMRMWSIYPTQIDAIVQAMQPDHSEVEKGCAV
                     LLAAQDASWGPIQHEGELHDRATYRGFWNIVQRARLSGQALPAEAIKRWFA"
     gene            4173..>4404
                     /locus_tag="PLG78_13150"
     CDS             4173..>4404
                     /locus_tag="PLG78_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE24113.1"
                     /translation="MARSQPRIGLALSGGGFRAAAFHIGVIKTLYDLGLYTSIRFVSS
                     VSGGAIVGAAIGLNQDRTVLDLLEVYLMNHSPL"
BASE COUNT         1151 a         1086 c         1095 g         1072 t
ORIGIN      
        1 gtgaggattc ttgcagcaca cgaaatcccg gccgtcaata ttctgggcgg agcgaggctt
       61 tctgaattct gatgattccc tggaataagc ccgcgccagc ggatgtaaac cggcaagatc
      121 cggaaaatgt ttttgccgca ggcaccagaa atcatcacat atccaataca gcctatccaa
      181 tagcgcataa cgttacttgg aagccatatc gagtggacag aaatgctaac gccagcctgg
      241 tagaatcagc gtttgcggac gcggagacga tctcgctcta ttcccacatt ccattttgcg
      301 agaccaggtg ttatttttgc gagtataccg tggtcggaaa aaaggagctc gatgccgcct
      361 ctgactatat gaaacttctt tcgcgggaac ttgccatgtt tcgcagtcta ctcggagagc
      421 ggagtctatc tggctttgat ataggcggcg gaactccttc ttttgtaaat gcgccactca
      481 tggcagagca tgtggatgag gtccggaaag cttttcgcac tgctcctggt tttgccatca
      541 gtattgaaac cactcccagg attgcgtcca cagaaccaga gaagatgaaa gcatacgcgg
      601 acatgggaat tgagcgcatt agcatgggga tccaggtgct gcagccggat ttactgaagc
      661 atctgggaag atctgaaaat ggcctggacc accatagaaa agcgtgtgac cacattcgag
      721 gggcaggctt caagaaattc aacattgatc tgatgtacgg attcgcaaag caaagtgacc
      781 agtcctggga agcgactctt cgtcatgcga ttgcactcga gccggattac attactctct
      841 atcgcatgcg atacaagctc actcgcattt ccgcagaagc agcggatgtc actcttgagc
      901 ctgtgctgcg tcaggcaaaa ctcgccagtg aaatgctcag gtctgcgggt tacattgcca
      961 atccgggcaa gaacacgtat tctagaattc caaaagacac ggggaccagc gcgtatttga
     1021 cgcgtcgcgt gatagaagga tctacctacc ttggcctggg gcttggcgca cagacattca
     1081 cagataccac catttcctac aatgacggag ccgcttccaa atccattgct ccttacgcaa
     1141 agagcatcgc ggaaggccgt ttgccgattc aggatctgta tcatctgcct ctcgagcata
     1201 tgatggccaa aatgattgct gtgagtttct attttggtga gattcgcgcg gacgcttttg
     1261 agaaaaagtt caaggtttct cttgaggaag catttccgcg tgagatcgaa tttgctttga
     1321 aagaaggtct catgcactgg agcatttccg aaaacgggca ggaaatccct ggatcatcag
     1381 accgtcgcgc tctctctctt actcctcacg gcgcccgaaa tttcaacgga accattgctc
     1441 ttttctttgc gccatccgtt cagtcttatc tgatccatcg tcagtctcag gcagacatgc
     1501 accgtcatgc ggcatctgcg caactggtgg cggataaaaa cgacgcagca tgagccacga
     1561 tttcgcaaac atactttttg ccgggccatg caatcgtttc tgtcctttct gcattggtaa
     1621 ggagcttccg gatcgggcca atgaagagaa tctgaatctg tatccacttc gaaaccagga
     1681 cgcattcatt cgcgaagtac agagactgga cattagagaa atcgtgatga ctgggaccac
     1741 tacagatccg cagctctatc gtcatgaaga aaggctgttg tctgagatgc gaaggcaatt
     1801 gcctcacgct cgtttctctg ttcacacaaa cggagtgctg gctcttcgta aactcgaagc
     1861 gttcaattcc tatgacagag cctgcatttc tttcccaagc tttcgttccg atacatatga
     1921 gaaaatgatg ggttcaagga ttgtcccgga tcttcgctcc attctcaggg aatcacggat
     1981 cccggtgaag gtcagttgcg tcatcaacga gcataacgtt cacgaattgg atgatttcat
     2041 tctggaagct cacagcatag gagttcgtcg tctggttctg cgaaaacttt ttggggaaac
     2101 cagaacctgg gatgtgctaa aggatcgcat tccagaatcc cattacaggg acaatccggt
     2161 ttacaacgtg gacggaatgg aagtaacgta ctgggacttt gatcgcacca caagcaaaag
     2221 catcaatctg ttcccggatg gaaccattgg accgagttat ctgcttgcgg caacgcctgc
     2281 atttatggct cgttcttctg cgattccggg atagggtttg acgccaaacc atccgagaag
     2341 tttctccata ccgcgccaga aattgattcg atccagaatc ggattcatcc atccgttggt
     2401 gatgcaatag aaagtatcaa acggctttgt gtggtgaacg tcatgatgga caatgccgag
     2461 tataatcttc cctttctgaa gtgtgcgaat ccaaagaggc ggagtatcca ggtgcgacca
     2521 tttgtgaaac tgattggtga gcactgtccc gccaatggtg aaactcaaaa agaaagccgt
     2581 catgcgatac gcatctgttg gatcaccgaa taacaacgga taaagaatcc agaatgtgat
     2641 gggaacggca gcaatgcatg tattgccaat agtcactgta aggctgtgag tggtaatccc
     2701 ctttggatct atgtgatgat cgcgaaatgg tttgatgaac gatcttccaa gcactggcat
     2761 agtctgagat ccgtatgtat caaagaacca gtggatgatt cctgtgatcc agtctgccgc
     2821 cagccatccc agaggcatta gcaagagtag ccagtagaga tttcgcgcgt tgttaaactc
     2881 aagcaccgaa agagcaagag tgctcgtaaa cacaatggga tacgtgtagg tagccagatg
     2941 ctcgaaaaaa acatgcactg gccccgcaga atgcgcaaat gcatcctctt ccatgattat
     3001 gtcgggtttg acagccgaat tctccatatg aatgcactca tgggaagtgg ctcggacttt
     3061 ggaatccttt tttatggaca cagggccctc ctttccaaaa tcaggaagga agaacaaaca
     3121 tcgaggcttc aaaaatgact caccccaatc aggcattgtt ttcgggcgaa aagccctttc
     3181 cagtgatccc aagttgcgaa catttcgcag gaaacgaaaa gatgatcggc aaggctctcc
     3241 agctgcagca ggagaaagga ccagtattcg atattactca ggactgtgaa gacggcgctc
     3301 ctcaaggcca ggaagtggaa catgcgaaaa tgatcgtacg catcaccaac tcttctgaaa
     3361 acaaactcgg catggcaggt gtgcgcatcc atgatcactc caatccacac tggaaacagg
     3421 atgtagatat tctggtgcca ggtgcaggag acaaactcgc ttacatcaca attccaaagc
     3481 caacagctgc ttcccaggtg gaagagatga tcgactacat tcgcaacgcc gcctccaaag
     3541 cgggtctgaa gcgcgagatt ccaattcacg tacttgtgga aactcatggt gcacttcgcg
     3601 atgcagataa gattgcagcc atgccatgga tgcaggttct cgactttggt ttgatggatt
     3661 tcatctcagg ccattttgga gccatcactc tttccaacat gaaaagcccc ggtcagtttg
     3721 atcacagatt gatttcgcgc gccaaagcaa acgtagttgc ggcagctctt gcaaatggaa
     3781 tcattccggc acacaacgtt actctggatc tcaagaactt tgagcaaacg aaatcagatg
     3841 caactcgcgc gcgctatgac tttggtttta tgcgcatgtg gtccatttac cctactcaaa
     3901 ttgacgcgat tgttcaggca atgcagccgg atcattcaga agtggaaaag ggatgcgcag
     3961 tgcttctcgc ggctcaagac gcaagctggg ggccaatcca gcatgaaggc gaactccatg
     4021 accgcgctac ctaccgcgga ttctggaata ttgtccaaag agcgcgtctt tccggtcagg
     4081 ctctgccggc agaggcaatc aagcgctggt tcgcgtaacc actgtttcac ggagcggcgg
     4141 actgccgctc cgatattcct cactcaattt agatggcccg ctcccaacct cgaataggcc
     4201 tggcactttc tggcggtgga ttcagggctg ctgcttttca tatcggcgta atcaaaacgt
     4261 tatacgatct tggtctctat acttctattc gatttgtttc aagcgtaagc ggaggtgcga
     4321 ttgtaggcgc ggcgattgga ctgaatcaag atcgcacggt ccttgatctt ctagaagtct
     4381 atctcatgaa tcattcgcca ctgg
//
LOCUS       DAOQJL010000316         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12000_length_4386_cov_4.347033, whole genome shotgun sequence.
ACCESSION   DAOQJL010000316 DAOQJL010000000
VERSION     DAOQJL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12000_length_4386_cov_4.347033"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLG78_13155"
     CDS             <1..1039
                     /locus_tag="PLG78_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24114.1"
                     /translation="NEGWLFQMKTSGGIVFCERSFLARLRHYQKAYGRRQEFTLSQYS
                     SFLIKWQHAHPDTFVAGEEGLALVLEQMQGLSMSADAWHAVLKMRVTDYNPRMLDSMF
                     LSGRFVWIRAITPGDPPRKTISRFVPLMILSRANLSLVKNPETSGLSHYAQTVLEFIR
                     RQGAVFFFDLQAQAGLFEDHLISGMKELVARGLLTADSLASLSSLFPDRKKARYLRRG
                     VGIAASGRWSLLRENETPQDQDIMIARILLRRYGVIFRYLAEKETLNLPWSILVRTLR
                     RLEMQGEIRGGRFIREAWGEQFAMPEALTLLKETAEGKPEDAPALSKTDPVRAVEKIM
                     QASRTSDTTAK"
     gene            complement(1049..2137)
                     /locus_tag="PLG78_13160"
     CDS             complement(1049..2137)
                     /locus_tag="PLG78_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24115.1"
                     /translation="MLRRLVLPLAVSIIPLLAGCMAAFGGSPTGNILDRIRSSPQYHE
                     KKFENEPPVAMIVEGKYFEMLRGEAFGGQVRTPPRPLPVVHPDVKSFSDPPAPGLRAI
                     WFGHASVLLEVDGVRIFTDPILSDRASPFENIGPQRFFSPPLSLEELPRIDAVVISHD
                     HYDHLDMKTIKFLATRGTMFYVPLGVGAHLTAWGIADSQVHEMDWWERLPLKSVEFIC
                     TPAVHYSGRGLFGGNQTLWSSWTIVGPQHRVFHSGDSGFSPHFKEIGQRFGPFDLTLI
                     KIGAYDPTWIYIHMDPEQAMSANVALGGRRMLPIHWGTFNLQIHSWDEPILRTKAAAE
                     SSHQDLVTPRPGEILDADKPYSSAEWWK"
     gene            complement(2253..3377)
                     /locus_tag="PLG78_13165"
     CDS             complement(2253..3377)
                     /locus_tag="PLG78_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNE24116.1"
                     /translation="MKKAIFFGLALSLTGIMTSANATVRKTANPVVFAHGLAGWDNIL
                     GAFYFGDNNGNFVGDPCDELLEVLCNGGLNSAQKAYAASVQPFQSSEVRGTQLADKIQ
                     SYMTSVGATYVNIIGHSQGGIDARKAAVVLKQRKGFAVVKTLVSVSSPHRGSPVAKYI
                     LDLKPGVVSVVDALAQIFGNVIYGSGNDGYAAAKQLVYNDYSSTDGVTTGMKAFNTAY
                     ASYTTAVSNRLASVITAQQGLDVNPALYILSKGFYDVDGDGASCAVASDDCDNDGALG
                     KGNGSRTDTDDDGLVGINSQQMGYRLKYTECFACDDYVTQDTSLGYLTDLNNPTSAQQ
                     SSHADVLNQDHLDVTGVGPDTMDENEFYAAIFDYMSANGG"
     gene            3842..>4386
                     /locus_tag="PLG78_13170"
     CDS             3842..>4386
                     /locus_tag="PLG78_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24117.1"
                     /translation="MKSKFWIIGLALLLILSSSCTAIKNAVGMGDKDDDNTLLLLSAA
                     YVLTRSTNPCTNSAYSSGTAVTTSTLSLSSGCVTGATSSMDSNLPTWIKNNFKCSVGY
                     VQSTYYCFKSKNLLNARSPYYKASTGLNGGSGTPNPNFIANQNLLYAIPMTPAKNNST
                     LSGTQNGLASIGIAVNGLAIF"
BASE COUNT         1128 a         1149 c         1090 g         1019 t
ORIGIN      
        1 aaatgaaggc tggctctttc agatgaagac gtcaggcggc attgtgttct gtgagagaag
       61 ctttcttgca cgcctccggc attaccagaa agcgtacggc cgccgccagg aattcactct
      121 ctcgcaatac agcagtttct tgatcaaatg gcaacatgcc catcctgata cgtttgtagc
      181 cggtgaagaa ggtctggcac tcgttctcga gcaaatgcaa ggattgagta tgtccgcgga
      241 cgcatggcat gccgtcttga aaatgagggt aacagattac aatcctcgca tgctcgactc
      301 catgtttctt tcaggccgat tcgtctggat tcgcgccatc actccaggag atccgccgag
      361 aaagacaatc tctcgatttg ttcctctgat gatcctttcg cgggccaacc tatctctagt
      421 gaagaatccc gagacctctg ggctttcgca ctatgcccag actgttctcg aattcattcg
      481 gcggcagggt gccgttttct tttttgatct gcaagcgcag gctggccttt ttgaagatca
      541 cttgatctcc gggatgaaag aactggtggc gcgcggactc ctcaccgcgg acagtctggc
      601 ttctctctca tctctttttc cggacagaaa aaaggcccga tacttgcgac gcggcgtggg
      661 gatcgcggct tccggtagat ggtcccttct gcgcgagaat gaaacgccac aggatcagga
      721 catcatgatc gcaagaatct tgctgcgcag atacggagtc atctttcgct acctggcaga
      781 aaaggaaact ctcaatctgc cgtggagcat actggtgaga actctgcgcc gtctcgaaat
      841 gcagggagaa atccgcggcg gaagattcat ccgcgaagcc tggggagaac aattcgcaat
      901 gccagaagct ctgacactct tgaaagaaac tgcagaaggc aaaccagaag acgctcctgc
      961 gctttcaaag acggatccag tgcgggccgt ggaaaaaatc atgcaggcca gcaggacttc
     1021 ggacacgacc gcaaaataaa cccgatccct atttccacca ttccgccgaa ctgtacggct
     1081 tgtcggcgtc taagatttct ccgggtctgg gtgtgaccag atcctgatga gacgattcag
     1141 cggcagcctt cgtgcgaagg atcggctcgt cccatgagtg aatttgcaga ttgaatgttc
     1201 cccagtgaat gggcagcatt cttcttccac caagtgcaac gttagccgac attgcttgct
     1261 ccggatccat gtgtatgtag atccaggttg gatcgtaggc tccgattttg atgagtgtta
     1321 aatcgaaagg cccaaacctc tggccaattt ctttgaaatg cggtgaaaaa ccagaatcgc
     1381 cgctgtgaaa gactcgatgc tgagggccca caatcgtcca tgaggaccag agagtttgat
     1441 tccctccaaa caatccgcgg ccggagtagt gaacggcagg cgtgcaaatg aactcaaccg
     1501 atttcaaagg cagacgctcc caccagtcca tctcatgaac ttgtgaatca gcaatccccc
     1561 acgcagtcaa atgcgcaccc acgccgagag gaacatagaa catggttccg cgcgtagcca
     1621 ggaatttgat tgtcttcatg tccagatgat catagtgatc atgcgaaatc acaaccgcgt
     1681 caatcctggg aagttcttcc agagagagag gtggagagaa aaacctctgc ggaccaatgt
     1741 tttcaaaagg agacgctcga tcggaaagaa ttggatctgt gaagattcgc acaccgtcca
     1801 cttccaggag gacggatgcg tgcccgaacc atatggcacg gagcccgggc gctggaggat
     1861 ccgaaaaaga ttttacatcc ggatgaacca caggcagagg cctgggcggc gtgcgcacct
     1921 gaccaccgaa tgcttccccg cgaagcatct caaaatactt tccttccacg atcatcgcaa
     1981 ccggaggttc attttcgaat ttcttttcat ggtattgagg cgaggaacga attcgatcga
     2041 gtatgtttcc tgtaggtgaa ccaccgaagg ccgccatgca gccagccagt agcgggatga
     2101 ttgaaactgc cagaggaaga acgaggcgac gtagcatggc attctaatgc cgcggctgcc
     2161 gaaaaaagca aacagttaac tgcccaaatg cggaaggatc acacaaaaaa gcaccgcctg
     2221 cgaacaggcg atgctgagtg ggggattatt tgttagccgc cgttcgctga catatagtca
     2281 aatatggccg cgtaaaattc attttcatcc attgtatctg gaccaacacc tgttacgtcc
     2341 aggtgatctt gattcagaac atctgcatgc gatgactgct gggcagacgt cggattgttc
     2401 aagtccgtca gataaccaag gcttgtatct tgcgttacgt aatcatcgca ggcgaagcat
     2461 tcagtgtatt tgaggcggta gcccatctgc tgggaattga ttccgaccag tccatcgtca
     2521 tctgtgtctg tcctggaacc gttcccttta cccagagctc catcgttatc gcagtcatcg
     2581 gacgccacag cgcacgacgc accatcgcca tctacatcgt agaaaccttt gctcaggatg
     2641 tagagtgcag gatttacatc cagtccttgc tgtgccgtga tgacagatgc cagtctgttg
     2701 gaaactgcag ttgtgtaact cgcatacgca gtgttaaatg ccttcatgcc ggtggtaacg
     2761 ccgtcagtag aactgtagtc gttgtaaacc agctgctttg ccgcagcgta tccgtcattt
     2821 cctgagccat agatcacgtt accgaaaatc tgagccagag catccacaac gcttacgaca
     2881 cctggtttca aatccagaat gtactttgct accggcgaac cgcggtgagg cgacgaaaca
     2941 gacacgagcg ttttcacaac cgcaaatccc tttctttgtt tcagaactac ggccgcttta
     3001 cgcgcatcga ttccgccctg cgaatgacca atgatgttca cgtaagttgc gcccacgctg
     3061 gtcatgtaac tctggatctt gtcagccagc tgtgttccac gaacctctga actctgaaac
     3121 ggttgaacag acgccgcgta agccttttgc gccgaattca aaccaccatt gcacaatact
     3181 tcaagcaact cgtcgcatgg atctcctacg aaattcccgt tgttgtcccc gaaatagaat
     3241 gcccccagaa tattatccca gcctgccagg ccgtgcgcga atacaaccgg gttggctgtt
     3301 ttgcggactg tggcattggc tgatgtcatg atcccggtca gactcaatgc aagaccgaag
     3361 aaaatggctt tcttcattct aaactcctcc tgtgcgaaat cacactggac atgaggaaag
     3421 cacggattgt accacaaagc cgggaaaaca tacaattttg agaagaacga gcgtacaact
     3481 aacgcctaac agaatgcgcc gcaaaaacga acagtatttg ccaatgacga tcggaaccgt
     3541 gcggtcaaaa aacagattca tccattccgg ggcaatagaa tcgcctatga atcggaagct
     3601 taggccaaga attccgaatc cctggattcc aggagcgtgg aatttccagg aacctggagg
     3661 gtaagcctgc agtgtcgcac aagagcaact acactgggac cgattggttc cgaaagcaag
     3721 cggtgacgct cattttcgcg cggcgttcat tttttattgc gatttgttat tgattctgag
     3781 acgccatctc gtcttataga gacgttcggc ggcgatgcga ggccgcgaag gagtctgaca
     3841 tatgaaatcg aaattctgga tcattggcct ggccctgctc ttgattctat cgtcctcgtg
     3901 cactgcgatc aagaatgcag tgggaatggg agataaagac gacgataaca ctctccttct
     3961 gttgagcgcg gcgtatgtgc tgacacgctc tacaaatcca tgtacgaact ctgcttattc
     4021 ttccgggaca gcagtcacca cctcaacgct gagtctgagt agtggttgcg tcactggagc
     4081 aacgagctcg atggactcca atcttcctac gtggatcaag aataacttca aatgctccgt
     4141 gggttatgtc cagagcacat actactgttt caaatcaaag aacctgctca atgcaagaag
     4201 tccctattac aaggcgtcca ccggattgaa cggcgggagt ggcacaccta acccaaactt
     4261 tattgccaac cagaatctac tttatgcaat tccaatgacg cctgccaaga acaacagtac
     4321 actttcaggt acgcagaatg gtcttgcatc cattgggatt gcagtgaacg gacttgccat
     4381 cttcaa
//
LOCUS       DAOQJL010000317         4364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12080_length_4364_cov_4.720121, whole genome shotgun sequence.
ACCESSION   DAOQJL010000317 DAOQJL010000000
VERSION     DAOQJL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12080_length_4364_cov_4.720121"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLG78_13175"
     CDS             <1..395
                     /locus_tag="PLG78_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24118.1"
                     /translation="PKLHPLGHQYLDLLQPPRLLEPPRGQTVDMTDKNELYFKWDPVR
                     GAEGYDFTLFRSYPDGRRTAILNLKTQRADFMLRDLGILDVGGFTWSVSAYGRNQVSE
                     PAASDFTITLRIDVGKPEIVTPGVQYTE"
     gene            392..2770
                     /locus_tag="PLG78_13180"
     CDS             392..2770
                     /locus_tag="PLG78_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24119.1"
                     /translation="MRALILWAGAIVFSLAPFTQRAYAQEVKTPFLEWKPIRGAAAYV
                     VQVRDTGGKVILEKTVGENKLEVELPPGKYQQRVSALNKFRKPTQYSDWVDFNIRVAE
                     LPNVETLKPTPLPVDVEKKGERAVAVVGTHLTESTKVFVDTDKGKIESTKVEVVGDNK
                     LIASSDSAKIDQGKHDIIIENPHNKIVRVENALKISEDKKVTTSSDPRVVREDAKKTD
                     RGRDTGREPGKDTGKDTGKDTGRDNGKVPAIEPMEIYPEYKPTKKQCGRAGICIPAPQ
                     KYAWNLTPQAFVPGLSRIRSGDTKGYIWPAVIGTFLVGAGYEDRHADKVAKRSQSDGL
                     GSIFATPLVLGTYSLWTNGGFLLIAQDQYKNIRNQRAEYRRTIQNRNILLAGALATFG
                     MAVIDGALMSDSQGFFYTTPGPGVGPIPGTRNLSNSTFTWKFTKDVWIPGKVRRNAGD
                     TAGTVTLGIFGALGAVTLYESYHQYQLVRTSRRLKDSVANQAVTNPILGVALASYWTN
                     PGFVYAVFDNNRQFTSLRNQYRRSTQVQVAAVGTAAFLYIFQVLDAASYSSSPGFFGP
                     APFEPGTNPPPMADMYCFGGPCVYSVTSNFRMRASAFIPGRTRIQNGDKKAGYMIAGT
                     MIGFGAAAVFEDYEANKARRAAIRAGNSGVNALFSSQLAVAAGYQYWTSSSYLFLYQQ
                     YYHDVRKQSRAYSRAVNARTTMLGGAAILYGLQFLDATTLPASNASISTEKQIAFSEA
                     ATFTQANVASHLLGDLKQDLRREAVHGLVQDRIEFKDRFPYDVAEFHKTFAF"
     gene            complement(2777..3763)
                     /locus_tag="PLG78_13185"
     CDS             complement(2777..3763)
                     /locus_tag="PLG78_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24120.1"
                     /translation="MRWILIPAVCFCCSFQTLRAQEVEDNANQDFPDLFAEGLAPQSY
                     PGGSFERARHDFVLCRGKTGKGDPPPGRPEEQVEGLSAGAYNNAVIDDVIRDIDATDA
                     GSAFRKKHLTIALEKMKIGLKVDPQFFPFLYNSGRLLLLLNLPEQALSYLLLARARLS
                     EFSAVHLFIGAAHARLGDGISATESYRVAYRRNPFDPSALVALGDFYLESGAFPQAHD
                     TYERVLKQFPENASARIGLGRLYAKKGEFVRARTFFESVVTENQDGSERSDYDRALHF
                     YFAEVCFSLKDYREAVKQYDRILKHPEDPFFLDNSLTLMKKRREIARKLAEE"
     gene            complement(3763..>4364)
                     /locus_tag="PLG78_13190"
     CDS             complement(3763..>4364)
                     /locus_tag="PLG78_13190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24121.1"
                     /translation="HVEKGDSDLRRIEKSIMGFGQLTDDFTMLRIGFKEAAAQQRSAD
                     ADQRIAAFKAEVRKARGLFNDGATDEAIFVLEQAMDMTEENADAMRLLVKIMIQAGMF
                     DRAANLSDSYTRRFPADTEFLYITSYAYAKSGETRQAIEFAERCRLRDPSMVRNLTHL
                     ARLHAQTGNEPLARRVLGEALQVDPLHAKARQMLAAIED"
BASE COUNT         1125 a         1170 c         1085 g          984 t
ORIGIN      
        1 tcccaaaatt gcatccactc gggcatcaat acctggatct tttgcaacct cctcgcctgc
       61 tcgaaccgcc tcgcggacag acagtggaca tgacggataa gaacgaactg tatttcaaat
      121 gggatccggt tcgcggcgcg gaaggctacg acttcacgct tttccgcagc tatccggacg
      181 gaaggcgcac cgccatcttg aatctcaaaa cacagagagc ggatttcatg ctacgggatc
      241 ttggaatcct tgatgtgggt ggattcacat ggtccgtctc tgcgtatggc cgcaatcagg
      301 taagcgaacc tgcagcgtct gattttacaa tcactctgcg aatcgatgtt ggtaagccgg
      361 agattgtaac gccgggagtc cagtacacag aatgagagcg ctgatcctct gggccggcgc
      421 gattgtcttc agcctggccc ctttcacaca acgagcttac gcgcaagaag tgaaaacgcc
      481 attcctcgaa tggaaaccaa ttcggggagc agcggcttac gttgtgcagg tgagagatac
      541 cggcggaaaa gtaatactcg aaaagactgt aggcgaaaac aagctggagg tagaactccc
      601 tcccggaaaa taccagcaga gagtttctgc tctcaacaaa ttcagaaagc cgacacagta
      661 ctcagactgg gttgatttca atatccgcgt ggcagaactg ccgaatgtag aaaccctcaa
      721 acctacacca ctacctgtgg acgttgaaaa aaaaggtgag agagccgttg ccgtcgtagg
      781 aacacatctc actgaatcca caaaagtctt cgtggataca gacaaaggaa aaatcgagtc
      841 cacaaaagta gaagtggtcg gggacaacaa actcatcgct tcgtctgatt ctgcgaaaat
      901 agatcagggc aaacacgata tcatcatcga aaacccgcac aacaaaattg tgcgcgttga
      961 aaacgcactc aagatcagcg aagacaaaaa ggtgacaact tcgagtgatc cgagagttgt
     1021 acgcgaagac gcgaagaaaa cggaccgcgg gcgagacaca ggaagagaac caggcaaaga
     1081 cactggcaaa gataccggga aagatacagg cagggacaat gggaaagttc cggccattga
     1141 gcccatggaa atttatccgg aatacaaacc aacgaagaag caatgtggac gagccgggat
     1201 ctgtatcccc gctcctcaga aatacgcctg gaatttgacg ccacaggctt tcgttccagg
     1261 cctcagtcgc attcgtagcg gcgacacgaa aggctatatc tggcctgcag tcatcggaac
     1321 gtttctggtg ggagcaggct atgaagatag acatgcggac aaagtagcga agcgatcgca
     1381 atctgacgga ctgggaagca tcttcgccac gcctctggtg cttggaacat attctctctg
     1441 gacgaacgga ggcttccttc tcatcgcgca ggatcaatac aagaacattc gcaaccagag
     1501 agcggaatat agaagaacca tccagaatcg caatattctc ctggcaggag ctctcgcaac
     1561 gtttggcatg gcggtcatag acggcgcgct tatgtctgac tctcaaggat tcttctacac
     1621 tacgcccggt ccaggagtgg gcccaatccc cgggacgcgc aacctctcta actctacatt
     1681 cacgtggaaa ttcacaaaag atgtttggat tccgggaaaa gtacgtagaa acgcaggaga
     1741 cactgcggga accgttactc tcggaatctt cggggcgctc ggtgccgtga cgctgtatga
     1801 aagctatcat cagtaccagc tggtgcgcac ttcgcgcaga ctaaaagaca gcgttgcaaa
     1861 tcaagcggtt acaaatccaa ttctgggtgt ggcgctcgcc tcctattgga ccaaccctgg
     1921 attcgtctat gcggtctttg ataacaacag acaattcact tcgctgcgaa atcaataccg
     1981 tcggagcacg caggtgcagg tagcagccgt cggcacggca gcttttctgt acattttcca
     2041 ggtactggac gcggcttcct actcgtcttc accaggtttc ttcggaccag ccccctttga
     2101 accaggaacg aaccctcctc ccatggcaga catgtattgc tttggagggc cgtgcgttta
     2161 ttctgttaca tccaactttc gcatgcgcgc ttccgcgttc attccgggaa gaactcggat
     2221 ccagaacgga gacaagaaag cgggatacat gatcgctgga accatgatcg gattcggtgc
     2281 cgccgcagtt tttgaagact acgaagcgaa caaggcgcgt cgcgcggcga tcagagcagg
     2341 caatagcgga gtgaatgctc tcttttcctc gcagcttgca gtcgcggccg gataccagta
     2401 ctggacttct tcatcttact tgttcctcta tcagcagtac taccacgatg tgcgaaagca
     2461 aagtcgagcg tactcacggg cggtaaatgc aagaactacg atgcttggtg gagcggccat
     2521 tttatacggg cttcaattcc tggatgctac cacattgcca gcaagtaatg cctccatttc
     2581 tactgaaaaa caaatcgctt tctcagaagc agccacgttt acgcaagcga atgtcgcgtc
     2641 tcatctgctg ggcgacttga aacaggatct gcgccgcgaa gcggttcatg gactcgtgca
     2701 agatcgaatt gaattcaagg atcgattccc gtacgatgtg gcggaattcc acaagacgtt
     2761 cgcgttttaa cttcgcctat tcttctgcca gctttcgcgc aatctcgcgt ctcttcttca
     2821 tcagcgtgag ggaattgtcc agaaagaatg gatcctccgg atgtttcagg atcctatcat
     2881 actgtttgac tgcttcgcga taatctttca gagaaaaaca tacttctgcg aaatagaaat
     2941 gcagagccct gtcatagtct gaacgttccg aaccgtcctg gttttcagtg acaacgcttt
     3001 caaagaacgt tctggcgcga acaaactcgc ccttcttcgc atacaatcgc ccgagtccaa
     3061 tgcgcgcgct tgcgttttct ggaaactgct tgaggactct ttcgtaagta tcgtgggcct
     3121 gcggaaaagc gcctgattcg agataaaaat cgccgagtgc gacgagcgcc gatggatcaa
     3181 agggatttct tctataggcg actctgtaac tttcagtcgc agaaatccca tctccgagtc
     3241 tcgcatgcgc ggcaccaatg aaaagatgca ccgcagaaaa ttcggaaagt cgcgctcgcg
     3301 caagaagtaa ataggaaagc gcctgctctg gaagattcaa aagaagcaag agtctgccgg
     3361 agttgtacaa aaatggaaaa aattgaggat cgactttgag cccgatcttc atcttttcaa
     3421 gtgcgatcgt caggtgtttc tttctgaagg cgcttcccgc atccgtcgcg tcaatgtctc
     3481 gaatcacgtc atcaatgaca gcgttgttat acgcacccgc agacaatcct tctacctgtt
     3541 cttccggcct tccgggaggc ggatctccct tccctgtttt cccgcggcaa agcacaaagt
     3601 catgccttgc tctttcaaag ctgccacccg gataactctg aggcgcaaga ccttccgcaa
     3661 acagatcagg gaaatcctga ttcgcattgt cttctacttc ctgcgcgcga agggtttgaa
     3721 acgaacaaca aaagcaaact gcggggatta gaatccagcg catcaatcct caatcgcagc
     3781 gagcatctgg cgcgcctttg catgaagagg atccacctgc agggcctccc cgagaacccg
     3841 acgcgcaaga ggctcatttc ctgtttgcgc gtgaagtcgc gcgagatgag ttagatttct
     3901 caccatggat ggatcgcgaa gacgacaccg ttccgcaaac tcgatggcct gtctggtttc
     3961 cccactcttc gcataggcgt acgaagtaat atagaggaat tctgtgtccg ccgggaatcg
     4021 tctggtgtaa ctgtccgaaa ggttggccgc acgatcaaac attcctgcct gaatcattat
     4081 cttcacaagc aatcgcatcg cgtctgcgtt ttcttctgtc atgtccattg cctgctcgag
     4141 cacgaatatg gcttcatccg tcgcgccatc gttgaatagc ccgcgagctt ttctcacttc
     4201 cgccttgaac gccgcaatcc tctggtccgc gtctgcgctt ctctgctgcg ccgccgcctc
     4261 cttgaagcca attcgcaaca ttgtgaaatc gtctgtgagc tggccaaaac ccatgatcga
     4321 cttctcaatc cgtctcagat cactgtcgcc cttttccaca tggc
//
LOCUS       DAOQJL010000318         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12173_length_4345_cov_4.068765, whole genome shotgun sequence.
ACCESSION   DAOQJL010000318 DAOQJL010000000
VERSION     DAOQJL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12173_length_4345_cov_4.068765"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLG78_13195"
     CDS             <1..800
                     /locus_tag="PLG78_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE24122.1"
                     /translation="RIALGAFIASLFFKTAVAPFHGWLLDVYKGSSYAALSIIGVAAK
                     VAGFGLLFKLLHGPFEPLRETWTSILGLAAIASFVFGGFQGLYQRNVKRILASSSVLN
                     AGFILLSMLGDGRQFTFYLAAYSAATLGVIAYLMSSGTAHADVDVVEDLHGASRKHPA
                     IALGFTVVLCSSAGVPLTAGFIAKFGVIYSMLQGPEKLPGLAAVAGALCSVLAFYYYF
                     KIIRAIWFESEIVTQVREQKRAYLIVCVLLVCALLAGGIYPPALTWI"
     gene            complement(797..1303)
                     /locus_tag="PLG78_13200"
     CDS             complement(797..1303)
                     /locus_tag="PLG78_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE24123.1"
                     /translation="MVGQSEFEDGFEDAALPGEFLRMTLKELPRRPLLSLPPTATIAD
                     AIQAMNDKRVGAVLLMEGNTLAGIFTERDVLKRVALSGIDVRTTSVSTVMTKKPDMLP
                     SDAGVAYALRKMNEEGYRHIPIVDKNGKATGIVAVRDIVRWLVELFPEELNLPPDPSL
                     ESRSVDGG"
     gene            complement(1395..2615)
                     /locus_tag="PLG78_13205"
     CDS             complement(1395..2615)
                     /locus_tag="PLG78_13205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNE24124.1"
                     /translation="MPQRALTFLFFTVLIDVIGFGVVLPVTPGLIMELNGTDLSSAST
                     VGGWLLFAFAITQFFFAPIMGNLSDRFGRRPVLLSSLAAFGLDYTITGFSPNLSWLFV
                     GRIIAGATGASFSTANAYIADISKPEDRAKNFGLIGAAFGLGFILGPALGGIVGGFGT
                     RIPFFVAGGLAFANALFGAFALPESLPESSRRLFQWARANPAGTLIHLKKYPMIYALA
                     AVNFLWQLAHQVLPSTWTFYTMLKFNWNQNDVGYSLAFVGLCMTIVQGGLSGPIIKKL
                     GERGAAIGGLLIGAIGFFGYALSPSPWIIYIFAAISSLSGLTYPSLNALMSGQVPATS
                     QGELQGGNSSLISITSILGPLLMTQTFHMFATGPGIVFPGAPFALAGILAIGALMVLF
                     ISLARIARAPGPQG"
     gene            2769..4178
                     /gene="sufB"
                     /locus_tag="PLG78_13210"
     CDS             2769..4178
                     /gene="sufB"
                     /locus_tag="PLG78_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNE24125.1"
                     /translation="METSVQSALDFYEPDNFPSGISRKVVESISHIKNEPGWVTEFRL
                     RALETYMSRPMPSWGFIPQFKIDLDSYVHYVGSRQKKKKSWDEVDPEVLRSFERLGIP
                     EHERRYLAGLEAMNDSETVYANVKKELADLGIIFCDIDTAIREYPEIVKKYIGSVVTP
                     EDNKFAALNSAVFSGGSFAYCPRGVKTPMPLQAYFKVTAASSGQYERTLLIADEGSEI
                     VYSEGCSSVQDSGTNFHTAVVELVAHKDARIHYTTIQNWKKNMYNWTVKRGIAHESAH
                     ITWTDVNIGAQTIKYPGIILAGDHATGDVLSLAFAGGGQIQDTGARIIHVGKNTRSNV
                     LAKGVSLDGGINSYRGLVKFEKGATGAMSHVKCDGLMMDARSESHAYPYNDVSGENGQ
                     LNYEATVSRINDDQLFYLQSRGLSEDEAKLLIVSGFCEGVVRDLNVEYSVEMTRLIRM
                     ILEDGKAIAAPEQAAAALP"
     gene            4199..>4345
                     /locus_tag="PLG78_13215"
     CDS             4199..>4345
                     /locus_tag="PLG78_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE24126.1"
                     /translation="MLLEIKGLEASIEGKEILKGVNLSIPPGEIHAIMGPNGSGKSTL
                     SYVLM"
BASE COUNT         1099 a         1060 c         1143 g         1043 t
ORIGIN      
        1 atcgcatcgc actgggtgcc tttatcgcat ctctgttctt caaaacagct gtggctccgt
       61 ttcacggatg gctcctcgac gtttacaaag gcagcagtta tgcggcattg agcatcatcg
      121 gcgtggctgc gaaagtggcc ggctttggac ttcttttcaa attgttgcac ggtccgtttg
      181 aaccgctccg cgaaacgtgg accagcattc ttggtctggc agccatcgcg tcattcgttt
      241 ttggcggatt tcaaggtctc tatcagcgca atgtaaaaag aatccttgca tcatcttctg
      301 tgctaaatgc cggattcatt ctcctgtcca tgctcggaga cggaaggcag tttacttttt
      361 acctcgcagc atactctgct gcaacgctcg gcgtgatcgc ctatttgatg tccagtggaa
      421 ctgctcatgc ggacgtggac gtagtagaag acttgcatgg agcgtcccgc aagcatcctg
      481 cgatagctct tggctttact gtggttcttt gtagttctgc gggcgttccg ctgactgcag
      541 gattcattgc gaaatttgga gtgatctact ctatgctgca ggggccagag aaacttccag
      601 gcctcgctgc ggttgccgga gctctctgtt ccgtccttgc attttattac tatttcaaaa
      661 tcatacgggc catctggttt gagtctgaaa tcgtaaccca ggtgcgtgag cagaagcgag
      721 cctacttgat cgtatgcgtg ctgcttgttt gcgcattgct tgcgggagga atctacccac
      781 ccgcacttac ctggatttaa ccgccgtcta cagatcttga ttcaagagaa gggtccggtg
      841 gtaagttgag ttcttccggg aagagttcta caagccatct cacaatgtct cgaaccgcca
      901 cgattccggt tgctttaccg tttttgtcta caatcggaat gtgtctgtaa ccttcttcgt
      961 tcatttttcg aagggcatac gcaacccctg cgtcgctcgg gagcatatct ggcttctttg
     1021 tcataacagt ggaaactgaa gttgtgcgca cgtcgattcc agagagggca acacgcttga
     1081 gtacatcgcg ctccgtaaaa atccccgcca gagtatttcc ttccattaac aaaactgcgc
     1141 cgactctctt atcattcatt gcctggattg catctgcgat cgtggctgtg ggaggaagag
     1201 aaagaagagg ccgtcgcggt agctctttca gagtcatgcg cagaaattca ccgggcagtg
     1261 ctgcgtcctc aaacccatct tcgaactcgc tttgaccaac catgtcccca tggtagaact
     1321 cattgatgca aacggtcaag tattcctgag cgtttttttg gacgggttct agaaaatccg
     1381 tctgtagttc agagtcagcc ctgtggtccc ggtgcgcgcg cgattcgtgc gagtgaaata
     1441 aaaagaacca tgagcgcgcc gattgccaga attccagcca gtgcaaatgg agcacccgga
     1501 aaaacgattc cgggacctgt ggcaaacatg tggaaagtct gtgtcattag aagcggtccg
     1561 aggatgcttg tgatgctgat gaggctggag tttccaccct ggagttcccc ttgcgaagtt
     1621 gccgggacct gaccggacat cagtgcgttc aaggatggat aggtgagacc actgagagaa
     1681 gaaatcgctg cgaatatata aatgatccag ggagatggag aaagtgcgta tccaaaaaaa
     1741 ccgatcgcac caatcaaaag acctccgatg gcagcgccac gttcaccgag tttcttgatg
     1801 attggaccgc tgagtccacc ctgaacaatt gtcatacaga gtcctacgaa ggccagggag
     1861 tatcccacat cattttgatt ccaattgaac ttaagcatcg tatagaaagt ccaagtggaa
     1921 ggaagtacct gatgcgcaag ttgccatagg aaattgactg cagcgagtgc gtagatcatt
     1981 ggatatttct tgagatgaat gagtgtaccc gctggatttg ctcgcgccca ctgaaaaagt
     2041 cttctggagg attccggcaa agactctgga agagcgaagg ctccgaacaa tgcattggca
     2101 aatgcaagtc cacctgccac gaagaagggg attcgcgttc caaatcctcc cacaatgccg
     2161 cctaacgctg gacccagaat gaaacccagc ccgaatgccg cgccaatcag tccgaagttt
     2221 ttcgcgcgat cttctggctt gcttatgtcg gcaatgtatg cgttcgccgt ggaaaaggac
     2281 gcgcctgtgg cgccagcgat gattcgtccg acaaagagcc aggacagatt gggcgaaaag
     2341 cctgtgatgg tgtagtcgag gccgaatgca gccagagagc tcagcaaaac cggtcgtctt
     2401 ccgaatcgat ctgatagatt tcccatgatc ggtgcaaaga agaattgagt gatggcaaat
     2461 gcaaagagca gccagccgcc cacagtggag gcactggata gatccgtgcc attcagttcc
     2521 atgatcagtc cgggtgtaac aggcagtacg acgccaaaac cgatcacatc gatgaggacc
     2581 gtgaagaaga ggaaagtgag cgctctttgc ggcatatgcc tgtcagcact gatacgcacc
     2641 gatttgcatg caacgatttc ggcggcgaaa tcgatccccg ggtgccttga aagcacacat
     2701 ctgacgggca aaacgatcca gtcaaaaaag gctttgtttg gagcctttct tcaattttac
     2761 tgtcggtaat ggagacatca gtgcaatcag cgctcgattt ctacgagccg gacaattttc
     2821 cgtccgggat ctcgcggaaa gtggtagagt ctatctctca catcaaaaac gaaccgggct
     2881 gggtgactga attccgtctt cgcgcactgg aaacatacat gtccaggcca atgccttcgt
     2941 ggggtttcat tcctcagttc aaaatcgacc tggattccta cgttcactac gtgggcagtc
     3001 gccagaagaa aaagaaatcg tgggacgaag tggatccaga ggtactgcgt agctttgaga
     3061 gacttggaat ccctgaacat gaacgcagat accttgcagg cctcgaagca atgaacgatt
     3121 cggagactgt gtacgcgaac gtaaagaaag aactggcaga tctgggaatc atcttctgtg
     3181 acattgatac agcaattcgt gaatatcctg aaatagttaa gaaatatata ggaagcgtag
     3241 tcactccgga agacaataag tttgccgcac tcaacagtgc agtctttagc ggcggatcct
     3301 ttgcgtattg cccgcgcgga gtgaagactc cgatgcccct tcaagcatat tttaaagtga
     3361 cggcagcatc ctccggtcag tatgaacgga ctcttctcat tgcagatgaa ggatcagaaa
     3421 ttgtgtattc ggaagggtgt agctccgttc aggattcggg aacaaacttt cacaccgctg
     3481 tggtggagct ggtcgctcac aaggacgcgc gcattcacta cactacaatc cagaactgga
     3541 aaaagaacat gtacaactgg actgtgaagc gtgggattgc gcacgaatca gcgcatatca
     3601 cctggacaga tgtcaacatt ggcgcacaaa ccatcaaata ccccggcatc atactggcag
     3661 gtgatcacgc aaccggagac gttctttctc tcgcttttgc cggaggtggc cagattcagg
     3721 acacgggagc tcgcatcatt catgtgggaa aaaatacacg ctccaatgtt cttgcgaagg
     3781 gtgtttctct cgacggcgga atcaattcgt atcgcggtct ggtaaaattc gaaaagggcg
     3841 ccacgggagc catgagtcat gtaaaatgcg atggcctgat gatggacgcg cgatctgaat
     3901 cgcacgcata tccgtacaac gatgtgagcg gagaaaatgg tcagttgaat tacgaggcta
     3961 ccgtttcacg tatcaatgac gaccagttgt tctatctgca atccagggga ctctcagagg
     4021 atgaagcaaa attgctcatc gtaagcggct tttgcgaagg cgtggttcgc gatctcaacg
     4081 tagagtactc ggtggagatg acgcgcttaa tcaggatgat cctggaggat ggaaaagcga
     4141 ttgcggctcc tgagcaggcc gccgctgccc taccttgaat caataaagga attttagtat
     4201 gcttctcgaa atcaaaggcc tcgaggcctc catcgaagga aaggagattc tcaaaggtgt
     4261 gaatctttct attcccccgg gagaaatcca tgcgatcatg ggaccaaacg gttccgggaa
     4321 gagcacgctt tcctatgtgc tgatg
//
LOCUS       DAOQJL010000319         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12179_length_4344_cov_4.344136, whole genome shotgun sequence.
ACCESSION   DAOQJL010000319 DAOQJL010000000
VERSION     DAOQJL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12179_length_4344_cov_4.344136"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLG78_13220"
     CDS             <1..718
                     /locus_tag="PLG78_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNE24127.1"
                     /translation="HILFNFKGRPTDDVEGQTSSVFERLFAYCKCMNLDPKKLVRTWI
                     YVNDIDNNYAGMVEARKSFFHAHGLTPDSGYPASTGIGGRSRDHRLSVLVNAHIYDNQ
                     TQVERIQALTHMNPTIEYGVTFERAMQVELDQGRYVYISGTASIDNEGCIVCELDLEG
                     QVNRTVENIAALLESCEMRLEDLMYVCIYLRDFNEEHRTRSCIQKLLPGNVPFVLLHA
                     PVCRPGWLIEIDGLARKTSL"
     gene            821..2080
                     /locus_tag="PLG78_13225"
     CDS             821..2080
                     /locus_tag="PLG78_13225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24128.1"
                     /translation="MHNTLEQILRQREVRGERTVALLRAAFISLAVFLDVLAFAHVIP
                     PYTSSPPAVSTLLLDAFFLSAALAFAILALKGVYYGFLKFVTITLDYVLILVMFAFDP
                     SLAGAGIENLWLAVIAPVFMLYLNLIRFSQSAAWYAAGLSVAQCAIVARLILGSWELQ
                     KILPLEFGLLMLISIGLAVSAAGRQMTEEASAKQMLERYLPPQLVKELQRSKASLVPG
                     GRRQFVTMLFADIRGFTRISETLSPEQVVEVLNDYLSTMTDVIFAHEGTIDKFIGDAV
                     MTIFGSPVSRPDDVVRAFRAAQGMILALDEFNARHPELPSPIAIGVGLHAGEVIAGNI
                     GSAKRLDYTVIGDNVNLTSRIEALTKHYGCRILASSAVIEGLKRCGVEPAAREIDTVI
                     VQGKTQSVVVYELFVEAAPATVSSTQF"
     gene            2566..3741
                     /locus_tag="PLG78_13230"
     CDS             2566..3741
                     /locus_tag="PLG78_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24129.1"
                     /translation="MRGATFLQGIAFLFAAGIGCTPALPVSANLDETTALVAGIGLLP
                     RSTTASATVITRVMVVLGNNTTTTNIYDPSTNMFSNGPTLTGSAAPGTIQFTIPSGTH
                     SGKTLIVHGASSSMYDPATGSMVAGPAPSGTLGIGSAAFVVPAGGAQPGKVMIVHGGS
                     LLTTSLYNPATHTMGAGAALGFGPTGGSFTMYIPTGTQGGKFMTMQAGAQRRAMFYDP
                     VGDSYATSANQTTANVGGDAWAYLMTSGANPNKIFVVVANGASTTNLYDLASDTWAAS
                     SPVLTAAASPGSNGFLVPSGGNSGKLLVLHGALTSSSFYDPSGNTMGGGPALTGNMGG
                     GSCVFSITAGPQMGKVMVVLGGNQTVTNIFDPSASTFSAGPSLTANANGGAQCFSIQ"
     gene            complement(3802..>4344)
                     /locus_tag="PLG78_13235"
     CDS             complement(3802..>4344)
                     /locus_tag="PLG78_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24130.1"
                     /translation="ERTVFEYRDKNESCCKFGVPAGRTGADVTVHLITDRAGMFESAY
                     PSLVLEYVTSVQDAGSVRKIDVNLATEHELVPTLFEIEAGYYDAFTTQKFPNSIPVSE
                     CCQGIHLKIRNEASTYQGDFGPVHFGIRIRKEYASIRSLPPEMNLIVTVETDKGVFKD
                     ARAVHLISYKSKGGVPFRFH"
BASE COUNT          967 a         1108 c         1160 g         1109 t
ORIGIN      
        1 tcatatccta ttcaatttca aggggagacc aactgatgac gtagagggtc agacaagttc
       61 tgtttttgag agactcttcg cttattgcaa atgcatgaat ctggatccga aaaaacttgt
      121 gcggacctgg atttatgtga atgatatcga caacaattac gcgggaatgg tggaggcgcg
      181 aaagtcgttt ttccacgcgc atggactgac gcctgactcc ggttatccgg cgagcaccgg
      241 gattggagga cggtcgagag accatcgcct atccgttctg gtgaatgcgc atatttacga
      301 caatcaaacg caagtggaac gaattcaagc actcactcac atgaacccca caattgagta
      361 cggtgttact tttgaacgtg cgatgcaggt ggaactggat cagggacgtt atgtttacat
      421 ttccggcact gcgagcattg acaatgaggg ttgcattgtt tgcgaattgg atctggaagg
      481 acaggtgaat cgaaccgttg agaatatcgc ggcgctgctt gagtcttgcg aaatgaggct
      541 ggaagacttg atgtatgtat gcatctactt gcgggacttc aatgaagagc acaggacaag
      601 atcctgcatt cagaaactct tgcctggtaa tgttccgttt gttttgttgc acgcacctgt
      661 ttgccgtcct ggctggctca tcgaaataga cggtctggca agaaaaacaa gtctttgact
      721 ttgccgcctt ctgccttctt ccgcgcgcgg tgagaacttt ctctgcttcg tctggaaaag
      781 gatggcctgc ctaccggcca ccggataaga gagcgtgtcc atgcacaata ctctggaaca
      841 aatcctgcgg cagagagaag ttcgcgggga aagaaccgta gctctcctgc gggctgcgtt
      901 catctcactt gccgtctttc tcgatgtcct tgcattcgct cacgtcatcc ctccctatac
      961 cagttcgcct cccgcagtga gcactctgtt gctcgatgcg ttttttcttt cggcagctct
     1021 ggcttttgcg atcctcgcac tgaagggagt ttactatggc ttcttaaagt ttgttaccat
     1081 cacccttgat tatgttctga ttcttgtgat gttcgcgttt gacccgtcgc ttgctggagc
     1141 agggatagag aacctgtggc ttgctgtgat tgcacctgtg ttcatgttgt atctgaacct
     1201 gatccgcttc tcacaatcgg cggcctggta tgcggcgggt ttgtctgtcg cacaatgcgc
     1261 gatcgttgcg cggttgatcc tcggttcctg ggaactgcaa aagattcttc cgcttgagtt
     1321 cgggctcttg atgctcattt ccattggact tgctgtaagc gccgcaggcc gccagatgac
     1381 ggaagaagcc agtgcaaagc aaatgcttga acgttatctg ccaccgcagc tggtaaaaga
     1441 attgcagcga agcaaagcaa gtctggttcc cggaggcagg cggcagtttg tcacgatgct
     1501 gtttgcagat atccgcggct tcaccaggat ttcagagacg ctaagtccgg agcaggtggt
     1561 tgaggttctc aacgactatt tgtccaccat gacagatgtg atttttgctc acgaggggac
     1621 cattgataag ttcatcggcg atgcagtcat gacgattttc ggatcgcccg ttagccggcc
     1681 agacgacgtg gtgcgtgcct ttcgcgccgc tcagggaatg attctagcgc tcgacgaatt
     1741 taacgctcgg catccagagc ttccgtcgcc cattgccatt ggtgtgggtc tgcatgcagg
     1801 agaggtcatt gccggcaaca ttggctctgc aaagcgactc gactacaccg tgattggaga
     1861 caacgtcaat cttacttcac gcatcgaggc gctgacgaag cactatggtt gcagaatcct
     1921 ggcaagcagt gctgtgatag agggactcaa acgctgcggt gtggaaccgg ccgcgcgaga
     1981 aattgatacg gtcattgtgc agggtaaaac gcagtctgtg gtggtgtacg agctgtttgt
     2041 agaggctgct ccggcgactg tttcttccac acagttttaa aaccgctgcc cacgtgcgtc
     2101 caggtcatct ggttgccctg aatgatcagg cggtaacgaa aatctgtgcc gattccgcaa
     2161 gaatgcgttg tatctgatat gatgtgtaag atgtcgcctt ctacagagta agaaaaagca
     2221 aagcagaggt cttcgtttcg aagataagtt ccgcgcccac catcgccgaa caaaactgca
     2281 tcccctgtcc aggtccatct gcccttgaga gtgttttctc ctgcaatgcg accgaccgat
     2341 gtctggcagg cacagaggcc tgccaaaaag attaaagcga ccagccgtcg aggggcccgc
     2401 gcgagtttca caatggagaa agagctgata tgcacgcgac tgcaaaccat attttgcttt
     2461 gactcacgtt tctacagcag cgaaagatcg tctaacgtac ccaaaaattt caagatgggt
     2521 ttcggaaata gattgaaaga gcccgagcca tacgtctact gagatatgcg tggagccacc
     2581 ttcctgcagg ggattgcctt tctgtttgca gccgggatcg gctgcactcc agctttgccc
     2641 gtttccgcta atctggatga gaccactgct cttgtggctg gaattggcct tcttcctcgt
     2701 tctaccactg ccagtgccac ggtaatcacg cgggtcatgg tggttcttgg aaacaacaca
     2761 acgaccacaa acatctacga cccttctacg aacatgttta gcaacggtcc cacactcacc
     2821 ggaagtgcag cacctgggac aattcagttt acgattcctt ccgggactca ttcgggcaag
     2881 acgttgattg tgcatggcgc atcctcctcc atgtatgatc cggctactgg ttccatggtg
     2941 gcggggcccg ccccttccgg gaccctggga attgggtcgg ctgcttttgt agtgcccgca
     3001 ggtggtgcgc agccggggaa agtcatgatc gttcatggtg ggagcctact caccacaagc
     3061 ctctacaatc cggccactca caccatgggg gcaggcgcgg ctcttgggtt tggcccaaca
     3121 gggggatctt tcacaatgta cattccaacc ggcacacagg gcgggaagtt catgaccatg
     3181 caggccggtg cgcagcgtcg tgccatgttc tatgacccgg tcggggactc ctatgccaca
     3241 agtgcaaacc agaccacagc aaatgtgggg ggagatgcct gggcatatct catgacatcc
     3301 ggtgccaacc caaacaagat cttcgtcgtc gttgccaacg gtgcctctac gacaaatcta
     3361 tacgatctgg catcggatac ctgggcggcc tcttctcctg ttttgaccgc cgctgcaagt
     3421 cccggatcaa atggttttct agttccatcg ggaggaaact ctggcaagct actcgtgttg
     3481 catggtgcgc ttacaagcag ttctttctat gatccttccg gaaacacaat gggtggaggt
     3541 cctgcactca ccgggaacat ggggggagga tcctgtgttt tttctatcac ggctggcccg
     3601 cagatgggta aggtgatggt ggtgctcggc ggcaatcaga ccgtaacaaa tatttttgat
     3661 ccttccgcat ccactttcag cgcggggcct tctctcaccg ccaatgcaaa cggtggcgcc
     3721 cagtgtttct ctattcagta gtacgatcgt ggatcgcgtg ctgtgcatgc tgaatagcgg
     3781 ttgcatgctc acgcaaacga ttcagtgaaa tcggaaagga actccgcctt tcgatttgta
     3841 gctgatgaga tgcacggccc ttgcgtcctt gaaaacccct ttgtccgttt caacggtgac
     3901 gatcaaattc atttcgggtg gaagagagcg aatgcttgca tactctttgc gaattctaat
     3961 tccgaaatga accggcccga aatctccctg ataggtactt gcttcatttc gaatcttcaa
     4021 atggattccc tggcaacatt cggatacagg gatagagttc ggaaattttt gagtggtgaa
     4081 tgcgtcgtag tagcccgctt ctatttcgaa taacgttggg accagttcat gttctgttgc
     4141 gagatttaca tcgatcttac ggacgctgcc cgcgtcctgt acagaagtca catactctag
     4201 cacgagagaa ggataggccg attcgaacat tccggctctg tctgtgatca agtggaccgt
     4261 gacgtctgcg ccggttcttc cggcgggcac gccgaattta caacaagatt cattcttgtc
     4321 tctgtattca aatactgtgc gctc
//
LOCUS       DAOQJL010000320         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12182_length_4344_cov_3.539753, whole genome shotgun sequence.
ACCESSION   DAOQJL010000320 DAOQJL010000000
VERSION     DAOQJL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12182_length_4344_cov_3.539753"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="greA"
                     /locus_tag="PLG78_13240"
     CDS             <1..1264
                     /gene="greA"
                     /locus_tag="PLG78_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE24131.1"
                     /translation="YLDQEESKDRLKRLHSYLFLDLAEKASDDHLGRKISKADMQEFL
                     KAEPVKGLLKISQDTPFPEFKRELIALILKVRPDYAEIVTELFFEVPGNLRTYIMNEL
                     ARLGQKDAVIRCIDRTLKKTREYPEIFLWIARAILSGQWTYDWMPWTRQDMLLNVFRF
                     LKPLAAIEKKGTKLKNQALEAVFGTTNITVESIKKSDLPELLRDAEAGNVRKMAVLFK
                     EISYVPDAQKENFMEFLSSVRPDLNMNAPDKAEEEAEESVSTADLFPAEGIILVSAKG
                     FADRKKHLDNLINVEMPANSRDIGEAQERGDLRENAEYKAAMERQTQLRAEITRVDEE
                     IKKAMIMDAAKIRTDIVTIGTAVTVENAGEKTVYQILGPWEADAEHNIISYLSPLGRA
                     LIGKKVGESATLEGGSAFKVQKIEKVSI"
     gene            complement(1324..2424)
                     /locus_tag="PLG78_13245"
     CDS             complement(1324..2424)
                     /locus_tag="PLG78_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE24132.1"
                     /translation="MSSQLNRLAVAVAERRAAGKRILDLHSPNSDFAISTVPADVLRT
                     AMDSYSRNLSYRPDAAGSLRAREAISTFYLKKFEIQVDPDHLILTASTSEAYSWIFRA
                     IPEGRIVAERPAYPLFEHLADFSRRRLDFVDHISPSDSPAAFLTVSPCNPSGTIVKQS
                     SHSRIAETGSLQVFDEVFSSYIWDDSLSGRHFPRPVGPSFTLNGASKLLRMPWLKIGW
                     IWLADHEKSAALQEDLLMIADTFLSVNGFAQDALPDLMDAGLASLPEYLRVLRSNCDF
                     VCSTISKIPGLSAKPPEGGLMLVVGLEEDLGDEEDFAVELLREHGLHVYPGYFYDLRE
                     SCMVLSFAHRREILEESLQILREFVEKKRAKG"
     gene            complement(2421..3143)
                     /locus_tag="PLG78_13250"
     CDS             complement(2421..3143)
                     /locus_tag="PLG78_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24133.1"
                     /translation="MRTWFNNNEGELDLDSHAPADTELARKHAQHRSLDAGLKKALGS
                     LHKDARHDASKFQKRLAAALADTKPELSFGEKISLDLDRFFSILRADSYAALRYATVL
                     IIPFVGIVVYSFSQKTTPTETAAVVMESPSGSALHEEMAPDADLSPQAKDDALHKMGG
                     AYPEQPVPRAAMIAADKEFEAVAQETLQIQEDLLKKNLEMAQNTAAKKSALDQLLDFY
                     TKNGNHTAAKEIKKELTKLSAH"
     gene            complement(3127..3708)
                     /locus_tag="PLG78_13255"
     CDS             complement(3127..3708)
                     /locus_tag="PLG78_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE24134.1"
                     /translation="MVEGREHESNLIRKATTGDHRAFLELIVPYQTRLLRRAQALLGS
                     MEDAEDAVQEALLMAYRSLAKFRGESGIYTYIYRIVINKCHDSVRKRRRTDSDSLDNV
                     PLISDERVSISKNHELSEESGYLIEKINSLDKKYRQVLLMRYYDDLSYQEIADLVHIS
                     VGTVKSRLFKARELLKRSIVRDGKGDEYFADMV"
     gene            complement(3708..>4344)
                     /locus_tag="PLG78_13260"
     CDS             complement(3708..>4344)
                     /locus_tag="PLG78_13260"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE24135.1"
                     /translation="VFVAVDLASKVWITDTLTYRVTEHQLENAVAGHVQMLVHGVGQI
                     NILGEEGKYIKLALVFNDRFVFGTGPAAPVFGLFLSLFAVIFLIFYRLHNPDFGPSAA
                     WLLVFSGAVGNLIDKMFVKSLETGAWVFSLGPKPNHVSGVVDFVECIWFGWTPGYDML
                     SFSLPLFGTFRPLAWLAWPVWPLFNFADSLIVVGTCMLVFSMLLTKPQPEA"
BASE COUNT         1187 a         1097 c         1079 g          981 t
ORIGIN      
        1 ctacctcgat caggaagaaa gcaaagacag actcaagaga ctgcactcct atctgttcct
       61 ggacctcgca gagaaagcat ccgatgatca cctcgggcgc aaaatttcaa aagcggacat
      121 gcaagaattc ctgaaggcag aacctgtaaa aggcctcctc aagatttccc aggacactcc
      181 atttccagaa ttcaaacgcg aactaattgc tctgatcctc aaagttcgtc ctgattatgc
      241 tgagatcgta acagaactct tctttgaagt tccgggcaac cttcgcactt acatcatgaa
      301 cgagctggca agactcggac agaaggatgc tgtgattcgc tgcatcgatc gcacccttaa
      361 gaaaacccgc gaatatcctg agattttcct ctggatcgct cgcgcgattc tttccggcca
      421 gtggacctat gactggatgc cgtggactcg tcaggacatg ttgctcaatg tgtttcgctt
      481 tctgaagccg cttgcggcca tcgagaaaaa gggaaccaaa ctcaagaacc aggcactgga
      541 agcagtgttt ggaacaacca acatcactgt agaatccatc aagaaaagcg atcttccaga
      601 acttctgcgc gacgcagaag cgggaaatgt tcgcaagatg gcagttcttt tcaaagaaat
      661 ctcatacgtt ccggatgcac aaaaagaaaa cttcatggag tttttatcca gcgtgcgtcc
      721 tgacctgaat atgaatgccc cggacaaagc ggaagaagaa gcagaagagt cagtctccac
      781 agcagacctg ttccctgcag aagggatcat tcttgtctcc gcgaaaggct tcgctgacag
      841 aaagaagcat ctggacaatc tcatcaacgt agagatgcca gctaactctc gcgacattgg
      901 agaagcgcag gaacgcggtg acttgcgcga aaacgcggaa tacaaagcgg ccatggagcg
      961 tcagacgcaa ttacgcgctg aaatcacgcg cgtagatgaa gaaatcaaaa aagcaatgat
     1021 catggatgcg gcaaagatcc gcacagacat tgtgaccatc ggcactgcag ttacagtgga
     1081 aaacgcagga gaaaaaaccg tataccagat cctcggaccc tgggaagcag atgcagaaca
     1141 caatatcatc tcttaccttt ctcctctagg gcgcgcgctc attggcaaga aagttggaga
     1201 aagcgctaca ctcgagggtg gatccgcatt caaggtgcag aagatagaaa aagtttccat
     1261 ctaagagaca tgcaagatga gcgggacctt tttaagttcc agtcgaacaa acgaagagcg
     1321 aggctaaccc ttcgctcttt ttttttccac aaactcgcga aggatctgca aggactcttc
     1381 tagaatttca cgcctgtgcg caaatgaaag aaccatgcac gattcgcgca agtcgtagaa
     1441 gtagccggga tacacatgaa ggccatgctc tctcagtagc tccactgcga aatcttcttc
     1501 atctccgagg tcctcttcga ggccaaccac gagcatgaga ccgccttctg gcggtttcgc
     1561 gctcaaacca ggaatcttcg aaatagtgga gcaaacgaaa tcacagttag atcgcaatac
     1621 gcgaaggtat tcgggcaagg acgcaagacc cgcatccatc agatcaggaa gagcatcctg
     1681 tgcaaaacca ttcacggaaa gaaacgtatc tgcaatcatc aagagatcct cctgaagggc
     1741 agcggacttt tcgtgatctg ccaaccagat ccagccaatc tttagccatg gcattcgcaa
     1801 gagcttgctt gccccgttca aagtaaaaga gggcccgact ggccttggaa aatgacgacc
     1861 ggacagagaa tcatcccaga tgtacgaaga gaagacttcg tcgaagacct gaagcgaacc
     1921 ggtctctgcg atcctggaat ggctgctctg ctttacaata gtaccggaag gattgcacgg
     1981 agaaacagtg agaaacgcgg ccggagaatc ggaaggagaa atgtgatcta caaaatccaa
     2041 tctgcgccga ctgaaatctg ccagatgttc gaagagcggg tacgctggac gctcggctac
     2101 gatccttcct tcggggatgg cgcgaaagat ccaggaatac gcttcgcttg tgctcgcagt
     2161 tagaatcaaa tgatccggat ccacctggat ctcaaatttc ttgagataga aggtggaaat
     2221 tgcttcgcgc gcgcggagtg atccagccgc gtccggtctg taagacaggt tgcgcgaata
     2281 agaatccatg gccgttcgca gaacgtcagc cggcactgtg ctgattgcga aatcggaatt
     2341 gggggaatgt aaatcgagga ttcgtttgcc tgccgccctt ctttctgcaa cggcaacagc
     2401 aagacgattg agctgtgagc tcaatgagcg gacaatttgg tcagttcttt tttgatttct
     2461 tttgctgcag tgtgattgcc gttcttcgtg tagaaatcaa gaagctgatc cagagctgat
     2521 tttttggccg cagtgttttg cgccatctcc aggttcttct tcaagagatc ttcctgaatt
     2581 tgaagtgttt cctgcgcgac tgcttcgaat tctttgtccg cggcaatcat agcggcgcga
     2641 ggaactggct gctctggata ggcaccaccc attttgtgaa gagcatcgtc ttttgcctgc
     2701 ggagaaaggt ctgcgtccgg cgccatctct tcatggagtg cagaaccgct cgggctttcc
     2761 atcaccacgg ccgccgtttc tgtaggcgtt gtcttttgag agaaggaata aactacgatg
     2821 cccacaaaag gaatgatgag aacggttgca tagcgcagag cagcataact gtccgcgcgc
     2881 agaatagaaa aaaagcgatc cagatccagg gagatttttt ctccaaaaga taattcaggt
     2941 tttgtatcag cgagagccgc agccagtctc ttctggaact tgcttgcatc atggcgcgca
     3001 tctttgtgca aagaacccag tgccttcttc aggccagcgt ccagagaacg atgttgcgca
     3061 tgcttccgcg caagctcagt atctgccgga gcatggctgt cgaggtccag ttcgccttcg
     3121 ttgttgttaa accatgtccg caaagtactc gtctcctttt ccgtctcgaa cgatggacct
     3181 tttgagtagc tccctggctt taaacaggcg gctcttcacg gttccaacgc tgatgtgtac
     3241 cagatctgca atctcttgat aggacaggtc gtcgtagtat ctcatgagga gcacttgcct
     3301 gtatttctta tcgaggctgt tgatcttttc tataagatag ccagattctt ctgaaagttc
     3361 atgatttttt gaaatgctca cacgttcatc ggaaatgagc ggaacattgt ccagggagtc
     3421 agagtccgtc ctcctgcgct ttcgcacgga gtcatggcat ttgttgatga caatgcgata
     3481 aatgtaggta taaatcccgc tttccccgcg gaatttggcc agagaacgat aggccataag
     3541 gagtgcttcc tggactgcgt cctctgcatc ttccatgctg ccaagcagag cctgagcccg
     3601 gcgaagaagc ctggtctggt agggaacaat caattccaga aaggcgcggt gatctcccgt
     3661 ggtcgctttc cggatcagat ttgattcgtg ctcgcggccc tccaccatca cgcttccggt
     3721 tgtggttttg tcaaaagcat gctgaagaca agcatgcaag ttccaactac gatcagacta
     3781 tctgcaaaat tgaacaacgg ccatacaggc caggcgagcc aggcaagcgg acgaaaggtc
     3841 ccaaaaagcg gaaggctgaa cgaaagcatg tcatatccgg gagtccaccc gaaccagatg
     3901 cactccacga aatctacaac accacttaca tggtttggct ttggaccgag agagaaaacc
     3961 caggctcccg tttcgaggga tttcacaaac atcttatcga tcaaattccc aacggcgcca
     4021 gagaacacaa gaagccaggc agcagacgga ccgaagtcag gattgtgaag gcgatagaat
     4081 atcaaaaaga taacagcaaa caaacttaaa aacagaccga acacaggagc ggcagggcca
     4141 gttccaaaca caaagcgatc attgaacacc agcgccagct tgatgtattt tccttcttct
     4201 ccaagtatat tgatctgccc gactccatgg acgagcatct gaacatgtcc ggccactgcg
     4261 ttttcaagct ggtgctctgt cacgcgatag gtcagtgtat ccgtaatcca gactttggat
     4321 gctagatcca ccgcaacgaa tacg
//
LOCUS       DAOQJL010000321         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12195_length_4341_cov_4.824778, whole genome shotgun sequence.
ACCESSION   DAOQJL010000321 DAOQJL010000000
VERSION     DAOQJL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12195_length_4341_cov_4.824778"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2746
                     /locus_tag="PLG78_13265"
     CDS             <1..2746
                     /locus_tag="PLG78_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE24136.1"
                     /translation="VCAPTQDMVLGIYILTSQLAGDVGEGKVFADIDEIMYATSVNAV
                     GFRAKVSVVHGGKMLQTTPGRVLFNATLPEGYPYVNRPVSDKEVNRIIADVYDKHGVA
                     AAVHMLDRVKDLGFHYATIFAPSISISDIRVSPKKADLISGANKEVADVQKAHKNGVI
                     TNDERYKKIIEIWTRTNELITESMFAELEKDQRGFNPIYIMAQSGARGSKQQIRQLAG
                     MRGLMARPSGEIIELAIRSNFREGLGVLEFFISTHGARKGLADTALKTADAGYLTRRL
                     VDISQDVIITEVDCGTENGVRLRAVKEGDKTIVSMGDRVFGRVVAEDVCDPVTSVVMI
                     PRGTLITKAHSAKINSMGIESVFIRSALTCDTRHGICTKCYGMDLARLKPVELGEAVG
                     TIAAQSIGQPGTQLTMRTFHVGGVATNVQVKESTYKLPHEAFILGIGGKLVTNPQKQQ
                     VFVNRGNINACRVSQVMDASKLINMRVEKGQAVVPGEIIASGYAGEEKSVTAESQGTI
                     DIRDGKFMILGEQMTIPVRAGAAIMVEAGQILDKNETMATFDPFNEVVISEHSGTVQL
                     QDIVEGKNLKKDEEGNFRIAEYKRERLSPRIVVGGTDYTVPLNSLLLVKDGQKIEQGT
                     ILVKITSGVEKTRDITGGLPRVEELFEARRPKDASTLAEIDGRIEDFNEVVKEKKVLY
                     IIPDSEEQEKIKVTIPVYKRVRVRHGDYVKGGDQLDEGTLDPHDILRIKGINALHEFL
                     IYEVQEVYRLQGVYINDKHIEIIVRQMLRKIEVEDPGDTNFVAHQQVDRFTFREENER
                     VVSEGGVPATARQILLGITRASLNTDSFISAASFQETTKVLTDAAIKGKKDALLGLKE
                     NVIIGHLIPAGTGLKHYRAVEIFKETYGDIERSYKEKEEEAQRALLTQTRIDIQSSGG
                     "
     gene            2996..3370
                     /gene="rpsL"
                     /locus_tag="PLG78_13270"
     CDS             2996..3370
                     /gene="rpsL"
                     /locus_tag="PLG78_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNE24137.1"
                     /translation="MPTISQLIRHGRKQQQNRTKSPALASSPQRRGVCTRVMTFTPKK
                     PNSALRKVARVRLTTGIEITAYIPGEGHNLQEHNVVLVRGGRIKDLPGVRYHIVRGAL
                     DTLGVDKRRQGRSKYGAKRPKA"
     gene            3374..3847
                     /gene="rpsG"
                     /locus_tag="PLG78_13275"
     CDS             3374..3847
                     /gene="rpsG"
                     /locus_tag="PLG78_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNE24138.1"
                     /translation="MSRRTGSSHKRSIIPDPVYNDEDIARFINYMMKDGKKSTAEKVF
                     YAALDILKEKTGREAVDVFREAVQNVKPELEVRSRRVGGVTYQVPVEVRPERMRTLAV
                     RWIVQNARDRKDHSMAEKLAYELLDAQKGSGGAVKKRDETRRMAEANKAFSHYRW"
     gene            3863..>4341
                     /locus_tag="PLG78_13280"
     CDS             3863..>4341
                     /locus_tag="PLG78_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE24139.1"
                     /translation="MAARKVSLEKTRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEV
                     HEGSTEMDWMEQEKERGITITSAATTTFWNGADKDREPHRINIIDTPGHVDFTVEVER
                     SLRVLDGAVAVYDGVAGVEPQTETVWRQADRYHVPRICFVNKLDRMGADFFFCVNSIR
                     "
BASE COUNT         1161 a         1074 c         1162 g          944 t
ORIGIN      
        1 agtgtgcgct ccaactcagg atatggttct ggggatttac atcctcacat ctcagctcgc
       61 aggagatgtt ggagaaggaa aagtattcgc tgacatcgac gaaatcatgt acgccacaag
      121 cgtaaatgca gttggatttc gcgcgaaggt ttccgtggtt cacggcggca agatgctgca
      181 gacgactcca ggtcgtgtgc tcttcaatgc aacgcttcca gaaggctacc cgtacgtaaa
      241 cagaccggtt tccgacaaag aagtaaaccg gatcatcgca gacgtttacg acaagcacgg
      301 agtagcggct gctgttcaca tgcttgatcg cgtcaaagac ctgggcttcc actacgcgac
      361 tatcttcgct ccgtccattt cgatttctga cattcgcgtc tctccaaaga aggcagacct
      421 gatttctggc gcgaacaagg aagtggcgga cgttcagaaa gcgcacaaga acggcgtaat
      481 tacaaacgac gaacgttaca agaagatcat cgagatctgg actcgcacaa acgaactgat
      541 caccgaatct atgttcgctg aactggaaaa agatcagcgc ggtttcaatc caatctacat
      601 catggcgcag tccggtgcgc gtggttccaa gcagcagatt cgccagctgg ccggtatgcg
      661 tggtctcatg gctcgtccgt caggcgaaat cattgaactt gccattcgct ctaacttccg
      721 cgaaggtctg ggtgtacttg agttcttcat ttctactcac ggtgcacgca agggtctggc
      781 ggatacagct ctgaaaactg cggatgcagg ctatctgact cgtcgtctcg tggacatttc
      841 tcaggatgtg atcatcactg aagtggactg cggaactgaa aatggtgttc gtcttcgcgc
      901 agtgaaagaa ggggacaaaa ccatcgtatc catgggagac cgtgtattcg gccgcgtggt
      961 tgcagaagat gtatgcgatc ctgttacttc cgtagtcatg atccctcgcg gaaccttgat
     1021 cacaaaagcg cacagtgcta agatcaacag catgggcatt gaaagcgtgt tcattcgctc
     1081 cgcacttacc tgcgatacca gacatggcat ttgtacaaaa tgttatggaa tggacctcgc
     1141 tcgactcaag cccgttgaac tgggcgaagc ggtcggaacg attgcggcgc agtcgattgg
     1201 acagccagga acccagctga ccatgcgtac gttccacgtg ggtggtgttg caaccaacgt
     1261 tcaggtgaag gaaagcacat acaagcttcc gcatgaagca ttcattctgg gcattggcgg
     1321 caaactggtc accaatccgc agaagcagca ggtctttgta aaccgcggga acatcaacgc
     1381 ctgccgcgta agccaggtaa tggacgcgtc caagctgatc aacatgcgtg ttgaaaaggg
     1441 ccaggcggtt gtgcctggtg aaatcatcgc aagcggatac gcaggcgaag aaaaatccgt
     1501 cacagcagaa tctcagggaa ccattgatat tcgcgacggc aagttcatga tcctcggcga
     1561 acagatgacc atccctgtga gagcaggtgc ggccatcatg gtagaagcgg gccagatcct
     1621 cgacaagaac gagaccatgg ctacattcga tccgttcaac gaagttgtga tctcagagca
     1681 cagcggaacc gttcagctcc aggacattgt ggaaggcaag aacctcaaga aagacgaaga
     1741 agggaacttc cgcattgctg aatacaagcg cgaaagactt tctcctcgca ttgtggtagg
     1801 cggcacagac tacactgttc ctctgaattc gctccttcta gtaaaagacg ggcagaagat
     1861 tgagcaagga accatccttg tgaagatcac ttcaggcgta gaaaagacca gggatatcac
     1921 cggtggtctt cctcgtgtgg aagaattgtt cgaagctcgt cgtccaaaag acgcttctac
     1981 tctggctgaa attgacggcc gtatagagga cttcaacgaa gtggtaaaag agaagaaagt
     2041 tctctatatc attccagaca gcgaagagca ggaaaagatc aaagtaacca tccctgtata
     2101 caagcgcgtg cgcgttcgcc acggcgacta cgtcaaaggc ggcgatcagc tcgacgaagg
     2161 cactcttgat ccgcatgaca ttctgcgaat caagggaatc aacgctttgc acgaattcct
     2221 gatctacgaa gtgcaggaag tttaccgcct ccagggcgtg tacatcaacg acaagcacat
     2281 cgagatcatc gtgcgtcaga tgcttcgcaa gatcgaagtg gaagatccag gcgatacaaa
     2341 cttcgtagct catcagcaag tggatcgttt cacgttccgc gaagagaacg agcgcgtcgt
     2401 ttccgaaggt ggggtgcctg cgactgcgcg ccagatcctt ctcgggatca ctcgcgcgtc
     2461 tctgaacacg gactccttca tttcagcggc ttctttccag gaaaccacga aggtccttac
     2521 ggatgctgcg atcaagggca agaaagatgc actccttggc ctcaaggaaa acgtgatcat
     2581 cggccatttg attcctgcgg gaactgggct gaagcactac cgtgccgtgg agatcttcaa
     2641 agaaacctat ggcgatatcg aacgctctta caaagagaag gaagaggaag cacagcgcgc
     2701 tcttctgact caaaccagaa tcgacatcca gtcctccggc ggctaatcgc cggagagtct
     2761 ctatgcgaca taaagcggcg agttttcgcc gctttttttg tctactgatt caaaaccgat
     2821 tgtcatagcg ggtcttagaa aaaatcatgg ccctgcgttg ttctgggctt aggccccgcg
     2881 ggaaaccgca tctgccaggg aagactgttc ctcacaggat gaggcaataa gttccgggct
     2941 tattaaaaaa tgaaaaacgc tgaattcggt agagataccg gtatgggaga agattttgcc
     3001 aacgatcagt caattgatcc gccacgggcg gaaacagcag cagaatagaa ccaagtctcc
     3061 ggcacttgca agttcgccac agagacgcgg ggtctgcacg cgagtcatga cattcactcc
     3121 aaaaaagcca aactctgcgc ttcgcaaagt ggctcgggtg cgtctgacaa ctggaatcga
     3181 aattactgct tatattccgg gtgaaggcca caaccttcaa gagcacaacg tcgtgctcgt
     3241 ccgcgggggc agaatcaagg accttcccgg tgtgcgttat cacattgttc gtggtgcgct
     3301 cgataccctc ggggtagaca agcgtcgtca aggacgctcg aagtatgggg ccaagaggcc
     3361 aaaggcttaa ggtatgtcac gtagaacagg tagctctcat aaaagatcca tcatcccgga
     3421 tccagtatac aacgacgaag atattgcacg tttcatcaat tacatgatga aggatggcaa
     3481 gaagtcaacg gctgaaaaag tattctatgc tgctcttgat attctcaaag agaagactgg
     3541 acgcgaagct gtggatgtat tccgcgaagc agttcagaat gtgaagccgg aactcgaagt
     3601 ccgttcccgc cgcgttggtg gtgtaacgta tcaggttcca gtggaagttc gtccggagcg
     3661 catgcgcact cttgcggttc gttggattgt gcagaacgcg cgcgatcgca aagatcattc
     3721 catggcagag aaacttgctt acgaactgct cgatgctcag aaaggcagcg gtggcgcagt
     3781 taagaagcgc gacgaaacaa gaagaatggc agaggcaaac aaggccttca gccactacag
     3841 atggtaataa ggatttaatt caatggctgc tcgcaaggtt tcgctcgaaa agacacgtaa
     3901 catcggcatc atggctcaca ttgatgccgg taagaccact actacagaac gtattctgta
     3961 ttacaccggt cgtacttaca agattggtga ggttcatgaa ggttccactg aaatggactg
     4021 gatggagcaa gagaaagaac gtgggatcac gattacttct gctgctacga ccacattctg
     4081 gaatggagcg gacaaggacc gcgagccgca tcgcatcaac atcattgata cgcccggcca
     4141 cgtagacttt actgtagaag tagagcgttc tcttcgcgtc ctcgacggcg cggttgccgt
     4201 gtacgacggt gtggccggtg tagagccgca gacagaaacg gtatggcgtc aggcggaccg
     4261 ctatcatgtt ccacgcattt gttttgttaa caaactcgat cgtatgggag cagacttctt
     4321 tttctgcgta aattccattc g
//
LOCUS       DAOQJL010000322         4324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12254_length_4324_cov_4.558210, whole genome shotgun sequence.
ACCESSION   DAOQJL010000322 DAOQJL010000000
VERSION     DAOQJL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12254_length_4324_cov_4.558210"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLG78_13285"
     CDS             <1..711
                     /locus_tag="PLG78_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNE24140.1"
                     /translation="QMAEGVQDKGGIALLDAILGGYTGRAMSPLATVDLVGLDVHKAI
                     VDNIYANTKDNAHETFKLPAYMQKLIDAGSLGNKAGKGLYRREKKADGTTDRFVYNIK
                     KGDYEPLAKIQVPFKKDAVTAIQASDYQKLGSIILTAPGMEADLLRHFIARYIAYSFS
                     LIPDVTDANGVDGAMGFGFNWVPPTAWVDVLGGVDAVRKFIEKEKIQVPEFLAKASGS
                     PFFSLRGKLEYRQLFRAG"
     gene            713..1195
                     /locus_tag="PLG78_13290"
     CDS             713..1195
                     /locus_tag="PLG78_13290"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE24141.1"
                     /translation="MLEAVRTFWKYNFWAQDQFFAVVSELTDEEFTRDLGDGNGSIRD
                     KLGHVCAADDVWMRRIEGEVSPPFKLDFTDKFNYIERQKAVRHKYHALIDGLSEVDLL
                     REISYKNLKGVEFTTPLVQILLHVSNHATYHRGQAASLIRRVKGKPPVTDMIEYFRAH
                     "
     gene            1216..2076
                     /locus_tag="PLG78_13295"
     CDS             1216..2076
                     /locus_tag="PLG78_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24142.1"
                     /translation="MKRCLVVPIFLLTACFPYFKQIEKDKQTFLDRFPNAHMYYESGG
                     RRMHYVRAGQGRDLIVFVHGSPGSWDAFARFLMNESLLSRARVISFDRPGFGESGNGT
                     AELSLERQAALIAEGVRLEQSGPAVRPGRIQPVSTGRVILVGHSFGGPVIARMAMDRP
                     LSYHALVFVAASVDPDLETVHWYQKAGNTGVVRFFLPAMLDVTNQEILPLRSELLAMI
                     PLWQNIKSRSYVIQGGRDDLVPPGNADFLEKNLRQVPLTVVRDPAMDHFVPWAHPDLI
                     EAALLDALAH"
     gene            complement(2101..3282)
                     /locus_tag="PLG78_13300"
     CDS             complement(2101..3282)
                     /locus_tag="PLG78_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24143.1"
                     /translation="MKEAKILIVEDENIVALNLQTRLKSLGYQVAGMASTGEDAVKKC
                     AETMPDLVLMDIMLRGEMDGVAAADQIREKYHVPVVYLTAYADDVTLERAKVTEPFGY
                     MLKPFEVKEIRTTIEIALYKHRMDRLLQESEERYSVAVHGSNDGIWDWDLVKNQVYYS
                     PRWKAILGCTDAEIGNTPEEWLSRVHPEEREALDMAIERHLAAVTPHMECEFRILAKN
                     GQHRWVFARGLAVRGTGGKAIRMAGSLADITRRHQAEEKLVRMTEHDPITDLLTESAF
                     LEKLYHAAGGSRDSTVMRLSLTKFNSVYAAQGPASAHDLAAHAAKVMISRLTSGAIAR
                     LFGGEFAILLADADPVRVKLLLTEITQDLDRLSIPCVTGFAILKAGGDYNLALCEAAK
                     Y"
     gene            complement(3292..>4324)
                     /locus_tag="PLG78_13305"
     CDS             complement(3292..>4324)
                     /locus_tag="PLG78_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNE24144.1"
                     /translation="RDVSARTEAEQQMKESEERLRLIVEHSPDAIAIHAAGTVIFVNP
                     RGVELLGAQKAGDILGRPILDFVPHEMRKTVIERIQTMIQKNEKVPMLEERFVRLDGK
                     AVDVEVIAIPLSVGGKPAIQVVARDISRRKAADAEIRKSLKEKEVLLKEIHHRVKNNL
                     QVIVSLLDLQSDRVEDRVALEALRECQNRVKSISLIHERLYESRDLAQVDFPEYIRML
                     MDNLLRSYGVEPDRVRMRIEVVDIRLGLDTAIPCGLIIHELVSNALKHAFPGGRSGEI
                     RVGIYADGDRNFLVVSDNGKGFGGVSDFRTVSSLGLQLVDTLTSQINGNIDFHTNGGT
                     EFIISFQQA"
BASE COUNT          986 a         1110 c         1080 g         1148 t
ORIGIN      
        1 caaatggcgg aaggggttca ggacaaaggc ggaattgctc ttctggacgc aatcctgggc
       61 gggtatactg gccgcgccat gagcccgctt gcaactgtgg atctggtggg tctggacgtt
      121 cacaaggcaa tcgtggataa catctacgcc aatacaaaag acaatgcgca cgaaacattc
      181 aagcttcctg cctacatgca aaagctgatc gatgcgggtt cattgggaaa caaggcagga
      241 aaaggtttgt atcgccgtga gaagaaagcg gacggaacca cagatcgctt tgtatacaac
      301 atcaagaaag gcgactatga gcctctggcc aaaatccagg ttccattcaa aaaggatgcg
      361 gttaccgcca ttcaggcttc cgattatcag aagctgggtt ctatcattct gactgcacct
      421 ggtatggaag cggatctgtt gagacacttc attgctcgtt acattgctta ctctttctca
      481 ttgattccag atgtgacaga tgcaaacgga gtagatggtg cgatgggatt cggtttcaac
      541 tgggttccac ctactgcatg ggtagacgtg ctcggcggag tggacgcggt gcgcaagttc
      601 atcgaaaaag aaaagattca ggttccagaa tttctggcaa aagcatccgg ttctcctttc
      661 ttcagcctgc gcggcaaact ggagtatcgt cagctgttcc gggcgggtta agatgctcga
      721 agcggtccgg acattctgga agtacaattt ctgggctcaa gaccagttct tcgcagtcgt
      781 ctctgaattg acggacgaag aattcacgcg agatctcgga gacggaaacg gatccattcg
      841 agacaaactc ggacatgtgt gtgctgcaga tgatgtctgg atgcgcagaa ttgaggggga
      901 agtgtcccct ccattcaaac tcgattttac ggataaattc aactacattg aacgccagaa
      961 agccgttcgg cacaagtatc atgcgctgat cgacgggctt tcagaagtgg atttgctccg
     1021 tgaaatttct tacaagaacc tcaaaggagt ggaattcacc actccgctcg ttcaaatttt
     1081 gcttcacgtt tccaatcacg ctacatatca tcgtgggcag gcagcgtccc tcattcggcg
     1141 ggtcaaaggc aaacctccgg taacggacat gatcgaatat tttagagcgc actagccgct
     1201 tgaggagcaa tcagcgtgaa aagatgtctg gttgttccca ttttccttct gacagcctgt
     1261 tttccgtatt tcaagcagat agaaaaagat aaacagacgt ttctcgaccg atttccaaat
     1321 gcgcacatgt actacgaatc cggcggcagg cgcatgcact atgtgcgcgc aggccagggc
     1381 cgggatctca tcgtattcgt tcatggctct cctggctcct gggatgcatt tgcacgcttt
     1441 ttgatgaacg aatctctact ttcgcgcgcg agagtgattt catttgatcg tccagggttt
     1501 ggcgaatccg gaaacggaac cgctgagttg agtctggaac gtcaggcggc attgatcgca
     1561 gaaggtgtgc gtcttgagca gagtggtcca gcagttcgtc ctggaagaat tcaaccagtc
     1621 agtactggac gcgtcatatt ggtaggccat tcatttggtg gacctgtcat tgcgcgcatg
     1681 gctatggatc gccccctgtc gtatcatgct ctcgtatttg ttgccgcttc cgtggatccg
     1741 gatcttgaaa cagtccactg gtatcagaag gcaggaaata cgggcgtggt tcgatttttc
     1801 ctgcctgcga tgctggacgt aaccaatcag gaaatacttc ctctccggtc cgagctactt
     1861 gcgatgattc ctctgtggca gaacattaaa tccaggtctt acgtaattca ggggggccgc
     1921 gatgacctcg ttccacctgg aaatgcagat ttcctggaga agaatttgcg tcaggtgccc
     1981 ctgaccgttg tgcgcgatcc tgccatggac cactttgttc cctgggccca tccggatctc
     2041 attgaggctg cactcctgga tgcactggct cattgagcta agggatgcca ttgatattct
     2101 tcagtacttt gcagcttcgc acagcgccag attgtaatct ccacctgctt tgagaatggc
     2161 aaagccggtc acacatggaa tagaaagccg atccaggtcc tgagtgattt cagtgagcaa
     2221 taatttcacc ctcactggat ccgcatctgc cagaagtatg gcaaactctc cgccgaatag
     2281 cctcgcaatg gcgcctgacg tcaatcttga gatcatcact tttgcagcat gtgctgccag
     2341 gtcatgtgca gatgcaggcc cctgggctgc ataaacagaa ttgaattttg tcaatgaaag
     2401 gcgcataacg gtagagtccc gacttccgcc ggcagcatgg taaagttttt caagaaacgc
     2461 tgattcagtc agaaggtccg tgataggatc gtgttccgtc attcgaacca gtttttcttc
     2521 tgcctgatgg cgccgcgtaa tgtcagccag ggatcctgcc attctgattg cctttcctcc
     2581 ggtgccgcgc acagccagac cgcgtgcgaa gacccagcga tgctgtccgt tttttgcgag
     2641 gattctaaac tcgcattcca tatgcggagt cacggcggcc agatgccttt cgatggccat
     2701 gtccagtgct tcgcgttcct ccggatgaac gcgcgacagc cactcctctg gagtgttacc
     2761 gatttctgcg tccgtgcatc ccaggatggc cttccatctg ggggagtagt acacttgatt
     2821 ctttacaaga tcccagtccc agattccgtc gtttgagcca tgaactgcaa cggagtacct
     2881 ttcttcactt tcctgaagca ggcgatccat ccggtgttta tacagggcga tctcaatggt
     2941 tgtgcgaatt tcttttactt cgaagggctt gagcatgtaa ccaaacggtt ctgttacctt
     3001 cgcacgctct agagtcacat catccgcata tgctgtgaga tagactacag gcacgtggta
     3061 cttctcgcgg atctggtctg ctgcggctac tccgtccatt tctccgcgta acatgatgtc
     3121 catgagcact agatcaggca tggtttctgc gcacttcttc accgcgtctt ccccggttga
     3181 ggccatgcct gccacctgat agcccagact tttgagcctg gtttgcaggt tcaacgcaac
     3241 aatgttttcg tcttcgacga ttaagatctt tgcctctttc atagtcgctc cttacgcctg
     3301 ctggaatgaa atgataaact ctgtaccacc gtttgtatgg aaatcgatat ttccgttgat
     3361 ttgcgaagtc aatgtatcca caagttgaag acccagcgag gatacagttc taaaatcgct
     3421 gacccctccg aatcctttgc cattgtctga caccactagg aaatttctat caccgtctgc
     3481 gtagatgcct actctgattt ctcctgaacg tccgcccggg aaagcatgct tgagcgcgtt
     3541 ggacacaagt tcatggatga tcagaccgca tgggatggca gtgtcgagtc ccaggcgaat
     3601 gtccaccact tcgattcgca tgcgcactct gtccggttcc acaccgtagg accgcagtaa
     3661 attgtccatg agcatgcgaa tgtactctgg aaaatctacc tgagccagat ctctagattc
     3721 gtagagtctt tcatggatca gggaaatgga tttcactcta ttctgacatt cgcgcaatgc
     3781 ttcgagtgcc actctgtctt ccactcgatc gctctgcagg tcgaggaggc tgacaatgac
     3841 ctgaagattg tttttcacgc gatgatgaat ttctttgaga agaacttcct tttctttgag
     3901 cgattttctg atttctgcat ccgccgcctt tctgcgtgag atatctcgcg cgaccacctg
     3961 aatggccggt ttacctccga cagacaatgg aattgcaatg acttctacgt cgaccgcctt
     4021 gccgtccaga cgcacgaatc tttcttcaag catgggaact ttttcgtttt tctgaatcat
     4081 ggtctggatc cgttctatga ctgtttttcg catttcgtgc ggaacaaaat cgagtatggg
     4141 tctacccaga atgtctccgg ccttttgtgc tcccagaagc tccactcctc gcgggtttac
     4201 aaaaatcacc gtgcccgctg cgtgaatcgc aatggcatct ggagaatgtt ccacgatcaa
     4261 acgcaagcgt tcttcgcttt ctttcatttg ttgttctgct tctgtgcgcg cagatacatc
     4321 acgg
//
LOCUS       DAOQJL010000323         4324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12255_length_4324_cov_4.445303, whole genome shotgun sequence.
ACCESSION   DAOQJL010000323 DAOQJL010000000
VERSION     DAOQJL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12255_length_4324_cov_4.445303"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLG78_13310"
     CDS             <1..1060
                     /locus_tag="PLG78_13310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE24145.1"
                     /translation="FAEFVRQELERNSIHVHLNTGLASVDQHEVRLEDGQVLPADLVI
                     LSMGVRPQTSMAKEAGLEIGPTGGVLVNGAMQSSDPSIFVVGDMAEIVHRITGKKVRV
                     PLAGPANRQGRIAGSNAAGARMTYAGAMGTSVVKICERTAAATGLTEKAAKEAGIQTS
                     SVFVHANHHAGYYPGAKQLTLKVVFETKTGQVLGAQAFGSEGVDKRIDVIATAIAGKL
                     TVEDLESLDLSYAPPFSSANDPVNTAGFIGANSRSGFSPVVSPDVFQTIDKSEIAILD
                     VRDDAETARGFVAGAIKIPLPQLRDRLNEIPRDRPVYAYCFSGMRSYLAARILMQNGF
                     EKVYNVAGGYRSIMRMAV"
     gene            1070..1414
                     /locus_tag="PLG78_13315"
     CDS             1070..1414
                     /locus_tag="PLG78_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE24146.1"
                     /translation="MRMEYWIYLIPVAIILWMIVRPGHAKKTEVKTMIESGSLIVDVR
                     SPQEFASGAYPGAINIPVDQVQARIGEFGDKGRPIVVYCQSGGRSQMAKQMLEAAGYK
                     KVVNGGGLRDMP"
     gene            1411..1920
                     /locus_tag="PLG78_13320"
     CDS             1411..1920
                     /locus_tag="PLG78_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24147.1"
                     /translation="MTWIAKRPGVAVEFIAKIFRRFSQSAAPLVEILSWSPRLLIAAA
                     LFEVFAPRNKKIARELKLIRLRVSFQVQCPWCVEMNSTDLKSANITEEELRFLSQGGP
                     VPSSLLPREMNVIRMAGVMSQSPVRMPQDLAHALDRDFSRSEIVTMTWTAAQVNFFAR
                     LFQSWVRNP"
     gene            complement(1949..3256)
                     /locus_tag="PLG78_13325"
     CDS             complement(1949..3256)
                     /locus_tag="PLG78_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE24148.1"
                     /translation="MKHTAVLIGAGNRGQIYAKYAKKHDLKITAVADPDPLKRQRIAA
                     LHEIPPSQQFSSMEHLLRGKRLADGAILATMDSMHATPAMKALKLGYHVMLEKPMGLT
                     IRQCEEVAAISQKQKKSVLLCHVLRYTSLFARIKDILSSGTIGNIASILHTENITPFH
                     MAHSYVRGNWRNSKTSSPLILAKCSHDLDLISWFADSRAIRVSSAGGISHFRPENKPV
                     DAAARCLDCRSVCPHNAYDLYVRATPMLRSFALSRSAAGKAARLLLLFPFLKRLIPGT
                     DPWTHWPTSTFSDGSKDQTIAALKDGPFGQCVYSGLNDQVDHQETWIEFENGTTASFR
                     FNGLAPQEGRTLRVDGSRGCLHATTMNGGSLKVVLHSGRIVRFKENFSLFGHAEGDEK
                     IVLEFKSVLDGHPGRSDARSSLESHRIAFAANESRLSGRVVKI"
     gene            3334..4209
                     /locus_tag="PLG78_13330"
     CDS             3334..4209
                     /locus_tag="PLG78_13330"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNE24149.1"
                     /translation="MRFVTIVLITLALFGCKQKSETKPSQEASAPVASEQAMSRGAAA
                     KSDDGKLVQDEDVVGGQPAQPGQALGHVGLVAPVGAPGRLLEYNVAVHYRSADLLKAR
                     ALLYSIASRRGFLRSSSASAESSQVSVEMAVRSTELYDTLKDLDAAGDLLTENISVTD
                     HTEADYGNSLKVKRENLRGARRTQAMTGDAAARNWAEREAALERSEDSSDQAQLEKWK
                     IQDHVNWATISVQVSGPDLPAPIRVPSYRNAFVGLLNVILELGYIAIYILPFLLIGLL
                     LIAKRRTWLGWIKRG"
BASE COUNT         1032 a         1013 c         1196 g         1083 t
ORIGIN      
        1 gttcgcagaa ttcgttcgac aggaactgga gcgcaattcg attcatgtgc atttaaacac
       61 tggccttgct tctgtagatc agcacgaggt tcgcctggaa gacggacagg tcttgccggc
      121 ggacctcgtg attctttcca tgggagtcag gcctcagacc agcatggcaa aagaggctgg
      181 ccttgaaatc ggcccaacgg gtggcgttct tgtcaatggc gccatgcagt cttctgatcc
      241 ttctattttt gttgtaggag acatggcaga aatagttcat cgcataactg gcaagaaggt
      301 gagagtgcct ctggccggtc ctgcaaatcg ccagggtaga attgccgggt ccaatgcagc
      361 tggagctaga atgacctatg ccggtgccat gggtacgtct gttgtgaaaa tttgcgaacg
      421 cactgcggcc gccacaggac tgactgagaa ggcggcgaag gaagcgggga ttcaaacatc
      481 ctctgttttc gtgcacgcga atcatcacgc gggttactat cctggagcaa agcagctaac
      541 gttgaaagta gtcttcgaaa caaaaactgg ccaggtgctc ggagcgcagg cttttggttc
      601 ggagggcgtg gataagcgca ttgatgtaat cgcaactgcg attgctggaa aattgaccgt
      661 ggaagatctt gaaagtctgg atctttccta cgctccgcca ttctcctctg caaatgatcc
      721 ggtgaacacg gcaggattca ttggcgcgaa cagtcggtct ggtttttcgc ccgtggtttc
      781 tccggacgtg ttccaaacca tagacaaatc ggagattgca attctggatg tgcgtgacga
      841 cgcggaaact gcacgtggat ttgtggcagg tgccatcaaa attcctcttc ctcagttgcg
      901 tgatcgtttg aatgaaattc cacgagatcg ccccgtgtat gcgtattgtt tttctggaat
      961 gaggagttat ctggcagctc gcattttgat gcagaacggt tttgaaaaag tgtacaatgt
     1021 ggccggcgga tacagaagta tcatgagaat ggcggtttga ggagatgaga tgagaatgga
     1081 atactggatc tatttaatcc cggttgcgat cattctatgg atgatcgttc ggcccgggca
     1141 tgcaaagaaa acagaggtaa aaacgatgat tgaaagtgga tcactgattg ttgacgttcg
     1201 cagtcctcag gaattcgcaa gcggagcgta tcccggagcg atcaacattc ctgtagacca
     1261 agtgcaggct agaatcggcg agtttggcga caagggcaga cccatcgtag tgtattgcca
     1321 gtcaggcggc agaagtcaga tggcaaaaca aatgctcgaa gctgcaggat acaagaaagt
     1381 tgtaaacggc ggcggattgc gtgacatgcc ttgacctgga ttgcgaaaag accaggcgtt
     1441 gcggtcgaat tcattgcgaa gatctttcgc aggttttcgc agtctgcagc gcctctggtc
     1501 gaaattcttt cctggtcacc gcggcttctt atagcagccg ccttgtttga agttttcgca
     1561 ccgcgaaaca agaagatagc gcgagagctc aagctcattc gtcttcgcgt gagtttccag
     1621 gtgcaatgtc cctggtgtgt tgaaatgaat tcaacagatt tgaaaagcgc aaacatcacg
     1681 gaagaggagc tgcgttttct aagccagggt ggaccggttc catcttcctt gcttccgcgc
     1741 gaaatgaatg tgattcgcat ggccggtgtg atgtctcagt ctcccgttcg catgccacag
     1801 gatcttgcgc atgcactcga ccgggatttc tccaggtcag agattgttac tatgacctgg
     1861 acggcggctc aggtgaattt ctttgcaagg ctgtttcaat cgtgggttcg caacccttga
     1921 attcgcggcc atgatagtat agatactctt agattttgac gactcttcct gagagacgag
     1981 actcatttgc tgcgaatgca atgcgatggc tctccaatga ggatctggcg tccgatctgc
     2041 caggatgtcc gtcgagtaca ctcttgaatt ccagaacgat cttttcatct ccttctgcgt
     2101 gtccaaacaa ggagaagttt tccttaaaac gaacaatcct tcctgagtga aggaccactt
     2161 tgagtgatcc accgttcatc gtggttgcat gcaggcagcc ccggcttccg tccactcgca
     2221 gagtgcgccc ctcctgtggc gcaagtccat tgaatcgaaa cgatgcggta gtcccgtttt
     2281 caaattcaat ccatgtttcc tggtgatcga cctgatcgtt taaccccgag tagacgcact
     2341 gtccaaaggg accatctttg agcgcggcga tggtttgatc tttgcttccg tctgaaaaag
     2401 tggaagttgg ccagtgggtc cacgggtctg tccctggaat gagtctcttg agaaatggaa
     2461 aaagaagaag aaggcgagcc gccttgcctg cagccgagcg gctgagcgca aaagaccgca
     2521 gcatgggagt tgcacggacg tagagatcat aggcattgtg cgggcaaacg cttctgcaat
     2581 ccagacagcg agctgctgcg tcgactggtt tattttccgg acggaaatgc gaaatgcctc
     2641 cagcggagct tacgcgaatg gccctggaat ctgcaaacca agaaatcaaa tctaggtcat
     2701 gcgaacattt cgcaagaatg agaggagaac ttgttttcga atttctccag ttgcctcgaa
     2761 cataggaatg cgccatatga aatggagtga tgttctctgt atgaagaatg gatgcaatgt
     2821 taccaatggt tccgctcgaa aggatgtctt tgattcgcgc aaacaatgac gtataacgta
     2881 gtacatgaca cagcaggacg gacttcttct gtttttggga aatcgctgcc acttcttcgc
     2941 actggcgaat cgtcagcccc attggctttt ccagcatcac atggtagccc agtttaagcg
     3001 ccttcatggc cggtgtggcg tgcatggagt ccatcgttgc aaggatggca ccgtctgcca
     3061 ggcgcttccc gcgcagcaga tgctccatgg aactaaactg ctgcgaaggc gggatttcgt
     3121 gcagtgcagc tatgcgctgg cgtttcaagg ggtctggatc cgcgactgcc gtgattttca
     3181 gatcgtgctt cttcgcatac tttgcgtaaa tctggccgcg gttgcccgca ccgatcaaca
     3241 cggctgtgtg tttcattttg cagataaatt ggaaagcatt cgcggagcgt agcaaaaaaa
     3301 cgaacagaaa agaaaacaac cggtctattc tctatgcgtt tcgtaactat tgttttaatc
     3361 actctggctc tttttggatg caaacagaaa tctgagacga agccttccca ggaagcatct
     3421 gcaccggttg cttcggagca ggcaatgagt cgaggggccg ctgccaaatc ggatgacggc
     3481 aagcttgtac aagatgaaga tgttgtaggt ggacagccag ctcaaccagg ccaggcactt
     3541 ggacatgtgg gtcttgtggc tccggttggc gctccaggta gactgcttga gtacaacgtt
     3601 gcagtgcatt atcgctctgc cgatttatta aaggcccggg cccttctgta ttctatcgcc
     3661 tccaggcgtg ggttccttcg cagctccagc gcgtctgctg aatcctctca ggtttctgtt
     3721 gagatggccg tccggtccac tgagttgtac gacacgttga aggatctaga tgcggccggg
     3781 gatcttttga cagaaaatat ttcagtaact gatcacacag aagcggacta cggaaacagc
     3841 ttgaaggtga agagggagaa ccttcgcggt gcaaggcgaa cccaggcaat gaccggtgat
     3901 gcagctgcgc gaaactgggc ggaaagagag gcagctctgg agagaagcga ggattcttcg
     3961 gatcaagcgc agcttgaaaa atggaagatt caggatcatg tgaactgggc tacgatttct
     4021 gtgcaggttt caggaccgga tttgcctgca ccgattcgcg ttccaagcta tcgcaatgct
     4081 tttgtcggtc tgctcaatgt aatcctggaa cttggataca ttgcaatcta cattctgccg
     4141 ttccttttga ttggcctttt gctcattgcg aaaagacgca catggctggg gtggatcaag
     4201 cgcggttaac gggaaagccg gcttctgtac atagaagccg gctaacgttt tgaattattt
     4261 tcaatcaggg tgcgcgcaat cgatttgtat tgcgcccttg tttcctgatt cagacagcca
     4321 aaga
//
LOCUS       DAOQJL010000324         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12286_length_4317_cov_4.898170, whole genome shotgun sequence.
ACCESSION   DAOQJL010000324 DAOQJL010000000
VERSION     DAOQJL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12286_length_4317_cov_4.898170"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLG78_13335"
     CDS             <1..331
                     /locus_tag="PLG78_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNE24150.1"
                     /translation="NRIIAGLSARVFLMQAPAKSGALITARYALEEGRDLICFDHPVL
                     ETWMNEGARRLIQEGATLLEIEALDSLFVRPPGIVWPPGNEQLEFFEKRQRGLLRVSD
                     TLYIEEV"
     gene            380..2521
                     /gene="topA"
                     /locus_tag="PLG78_13340"
     CDS             380..2521
                     /gene="topA"
                     /locus_tag="PLG78_13340"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNE24151.1"
                     /translation="MAVKTKEETAPKKKEAKKSIKASSKGKTLVIVESPAKAKTINKY
                     LGNSYIIEASMGHLIDLPRSRMAVNVEQNFEPEYITVRGRAKILNKLKKIAQHSDRVL
                     LAADPDREGEAISFHLARALGPANTDIKRIEFNEITKSAVQEAVRHPRDINMDLVNAQ
                     QARRILDRLVGYNISPLLWKKIKRGLSAGRVQSVALKLICDRESEIDKFVPEEYWTLD
                     VKFNVGKKGFAASLSSVDGKKADLKSKEEVDSILARMEGLSYNVASVTTKERRRQPPA
                     PYTTSRLQQDGTNRLGFTSQKTMMVAQQLYEGLELGGTTTGLITYMRTDSTRVSPGAV
                     EQLRGYIKDSLGENYLSPDIRQFKSSKSAQDAHEAIRPTDPARHPDDIQKYLSKEQHR
                     LYRMIWENFVASQMADEVADQTTAEIAGDGLIFRANGRKVKFPGFTEIQSPDEKKKKE
                     KETELPALEVGQNAVLEKTLPEQHFTQPPPRFTDASMVKTLEESGVGRPSTYAPTIMT
                     LLKRYYVVRKQRALQPTELGKLVNSVMAVHFRELVDSGFTARMEDDLDRIAGSELAWQ
                     DMLRTFYSPFVDILKNATEQIGEMRNVLDEVTEYVCEKCGKQMVKRIGRNGYFLACPG
                     FPECKNAKPIPLGKCPKCADGLVVQKATRRGRPFFSCNRYPECDFSTWDKPTGELCPN
                     CGKLLLEKSSREKGKYIACCACDFEEAEKSA"
     gene            2563..3915
                     /locus_tag="PLG78_13345"
     CDS             2563..3915
                     /locus_tag="PLG78_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNE24152.1"
                     /translation="MLRFGLAAAFALSFLSSPLFAWSEHAMLGYYGLITMLEIKNAQP
                     VPAESLESFLQKEEAGLEKVLADIEEWNKKNLVVYPVPTPEIAFKRTGIDHKRRFLEA
                     LRMDPESRTPLYLQLLPGQEAGGRPAFPVKDISVFKNPMWIAQTRFTTIREGEAVLPL
                     EVVASACDEPDYGFDIGIWQDNNSPHSKKFNMGIQPFGNPNLEYGSQAPVHMGFYHEA
                     GIIYLLAGFLKRTYPEMRVHQAYALSRFAFRTGHPYWGFRFMGWGLHYVQDLTQPYHA
                     RVLPGVGTLRMLWINFMDIIGRHEGKKNIVQLVSNRHLALENFQRRIMTRDLRNKDLN
                     QPYWFALQNTEFDSKYNHFNKKYLRDVLTEESVSKSDELSREIMALMPVRLVDDPGVE
                     FEEEKGSMDLLRTMETNQPGSVSQFANVLARLTGAAGVHSRNYVRAILATQDTYDSVN
                     "
     gene            complement(3908..>4317)
                     /locus_tag="PLG78_13350"
     CDS             complement(3908..>4317)
                     /locus_tag="PLG78_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNE24153.1"
                     /translation="LTGNEHRSILADVQAIAVAGAPSNLETLVSNPAVYALLGGWKSD
                     KPRAYSFASPVQYATPDDPPVLLMHGKYDMIVPESQSKAMAEKLASAGVQVEFRELPY
                     GHMRTTLTYNGSEVAAAVRFFKETLQPAQAAVN"
BASE COUNT         1169 a         1028 c         1103 g         1017 t
ORIGIN      
        1 aaatcgaatc atcgccggac tttctgccag agtatttctg atgcaggcgc ctgcgaagag
       61 tggcgctctg atcaccgcac gttatgcttt agaagagggt cgggacctga tttgctttga
      121 tcatcctgtg cttgagacct ggatgaatga gggagccagg cgactcatcc aggaaggggc
      181 cacattgctc gagattgagg ctctggattc tctatttgtg cgtccaccgg ggatcgtctg
      241 gccgcccggg aatgagcagc tggagttttt cgaaaaaagg cagagggggt tgcttcgtgt
      301 ctccgatacc ctttatatag aagaagttta aaaaaagagt ggcgcgattg cgcggccggg
      361 tccccggaga cataaagtca tggcagtaaa aacaaaagaa gagacagctc cgaaaaaaaa
      421 agaagccaaa aagagcatca aagcatcctc caaaggcaaa actctcgtca tcgtagaatc
      481 gcctgcgaag gcaaagacaa tcaacaaata cctggggaat agctacatca ttgaagcctc
      541 catgggccac ctgattgacc tgccgcgttc gcgtatggcc gtaaatgtgg agcagaattt
      601 cgaaccggaa tacatcaccg ttcgcggccg cgccaagatc ctgaacaagc tgaagaaaat
      661 cgcacagcat tccgatcgcg ttctacttgc ggcggacccg gatcgcgaag gagaagccat
      721 ttcatttcac ctggcgcgtg ccctcggccc ggccaacacg gacatcaaac gaattgaatt
      781 caatgaaatt actaagagcg ccgttcaaga agctgtgcgt catcctcgcg acatcaatat
      841 ggatctggtc aacgcgcagc aggcgcgtcg tatcctggac agacttgttg ggtataatat
      901 ttctccgttg ctctggaaga agatcaagcg cggcctctct gccggtcggg ttcagtctgt
      961 tgcgttgaag ctgatttgtg atcgagaaag tgaaattgat aagtttgttc cggaagaata
     1021 ctggaccctc gacgtaaaat tcaatgtagg taagaaagga ttcgcggcca gcctttcctc
     1081 agtggacgga aaaaaggcgg atctcaagtc aaaagaggaa gtggattcca ttctggccag
     1141 aatggaagga ttgtcgtata acgttgccag cgtgactaca aaagagcgca gacgtcaacc
     1201 acctgcgcct tacacaacat cgcgtcttca gcaagacgga acgaacaggc tgggctttac
     1261 ttctcaaaaa acaatgatgg tggcacagca gctctacgaa ggccttgaac tgggtggaac
     1321 taccaccggt ctgattactt acatgcgtac tgactccaca cgcgtttctc ctggagctgt
     1381 agaacagttg cgtggctata tcaaagattc tctgggagag aattatcttt cgccagacat
     1441 tcgtcagttt aagtcttcga aatctgccca ggacgcgcac gaagcaattc gtcctacaga
     1501 tcccgcgcgt catccggatg atattcagaa atatctttcc aaagaacaac atcgtcttta
     1561 tagaatgatc tgggaaaact ttgtggcctc tcagatggcg gatgaagtgg cagatcaaac
     1621 cactgcagag attgcaggag acgggctcat atttcgcgcg aacggtcgca aagtgaagtt
     1681 tccgggcttt acagagattc agtctccaga tgaaaaaaag aaaaaggaaa aggaaacgga
     1741 actgcccgca ctggaagtgg gacagaatgc tgtacttgaa aaaactcttc ctgagcaaca
     1801 ctttactcaa ccgcctccgc gtttcacgga tgcatctatg gtgaaaactc tagaagaaag
     1861 cggagtgggt agaccatcta cgtacgcgcc caccatcatg actttgctca agagatacta
     1921 tgttgtgcga aagcagcgtg cacttcagcc aacggaactt ggcaagctgg tcaacagtgt
     1981 gatggcagtt cattttcgcg agctggtgga ttcaggattt accgcgcgca tggaagatga
     2041 tctcgataga attgcaggtt cagagctcgc ctggcaggac atgcttcgaa cgttttattc
     2101 tcctttcgtg gacatattga aaaatgcaac cgagcagatt ggcgaaatgc gcaacgtgct
     2161 cgacgaagtt acagagtatg tctgcgaaaa atgcggcaaa caaatggtca aaaggattgg
     2221 cagaaacggc tacttccttg cctgtcctgg ctttcctgag tgtaagaacg caaagccaat
     2281 cccactcgga aaatgtccaa aatgtgcaga cggactcgtt gtgcagaagg caaccaggag
     2341 aggtcggccg ttcttcagtt gcaatcgcta tccggagtgc gatttctcaa catgggataa
     2401 gccaaccgga gaactctgtc cgaattgcgg taagcttctt ctggagaaat catcgcgcga
     2461 gaaaggcaaa tacattgcct gttgcgcctg tgactttgaa gaggcagaga agtcggcctg
     2521 aaaagtaatt gtaatatata taacaaacta tcaacctgga gcatgcttcg ttttgggttg
     2581 gccgccgctt ttgcactctc attcctatct tctccgctct tcgcctggag cgaacatgca
     2641 atgcttggct attacggcct cattaccatg ctcgagatca agaacgcaca acctgttccg
     2701 gcagagagcc tggagtcctt tctacagaag gaagaggcag gccttgaaaa ggtgctcgca
     2761 gacatagaag agtggaacaa gaagaacctc gtcgtttatc cggtgcccac acctgagatc
     2821 gcattcaaga gaacgggcat agatcataag agaagatttc tggaagcgct tcgcatggat
     2881 ccggaatctc gcactcctct gtatctacag ctattgcccg gccaggaagc aggaggtcgt
     2941 cccgcatttc cagtcaaaga cattagcgta ttcaaaaatc caatgtggat tgctcagacc
     3001 agattcacaa ccatacgcga aggagaagca gttcttcctc ttgaggtggt tgcgtctgca
     3061 tgcgatgaac ccgattatgg atttgatatt ggaatctggc aggacaacaa ttcacctcac
     3121 agcaagaaat tcaacatggg cattcagcct tttggaaatc ccaacctcga atacggaagt
     3181 caggctccgg ttcatatggg gttttatcac gaggcaggga tcatctatct tctcgccgga
     3241 tttttaaagc gcacgtatcc tgaaatgcgc gttcaccagg cgtatgctct ttctcgtttt
     3301 gcattcagaa cgggtcatcc atactggggc tttcgcttta tgggctgggg tttgcactac
     3361 gtgcaggatc tcactcagcc atatcacgca cgagtccttc ctggcgtggg aactctcagg
     3421 atgctctgga tcaatttcat ggacatcatt ggaaggcacg aaggtaagaa aaacatagtg
     3481 cagcttgtat ccaatcgcca tcttgccctg gaaaatttcc agagaagaat catgactcgc
     3541 gatttgagga acaaagatct aaatcaacct tactggtttg cgttgcaaaa cactgagttc
     3601 gattcaaagt acaatcattt taacaagaaa tatttgaggg atgtcctcac agaagaatcc
     3661 gtgagtaagt cagatgaact cagccgcgaa atcatggcac tcatgccagt tcgtctcgta
     3721 gatgacccag gtgtagaatt tgaagaagaa aagggatcca tggacttgct tcgcaccatg
     3781 gaaacaaacc agcctggatc tgtaagtcag tttgcaaatg tactggccag gctgacaggt
     3841 gcggccgggg ttcattcgcg taactacgtg cgcgcgattc ttgccaccca ggatacgtac
     3901 gattctgtta attgactgcg gcttgcgctg gctgtagagt ttctttgaag aatctcacgg
     3961 cagccgccac ttcggatcca ttgtaagtaa gagtagtgcg catgtgtccg tagggcagtt
     4021 ctctaaattc aacctgtacg cccgcggatg caagtttctc tgccatcgcc ttagactgac
     4081 tttctgggac gatcatatca tatttcccgt gcatcagcaa aacaggagga tcatccgggg
     4141 ttgcgtattg cacgggagaa gcaaagctgt acgcgcgcgg tttatctgat ttccatccgc
     4201 ctaacagtgc gtagacggcc ggattggata ccagtgtttc cagattggaa ggcgcacctg
     4261 caactgcaat tgcctggaca tctgcgagaa tggacctgtg ctcgtttcct gtgagac
//
LOCUS       DAOQJL010000325         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12409_length_4291_cov_3.664306, whole genome shotgun sequence.
ACCESSION   DAOQJL010000325 DAOQJL010000000
VERSION     DAOQJL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12409_length_4291_cov_3.664306"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1106
                     /locus_tag="PLG78_13355"
     CDS             3..1106
                     /locus_tag="PLG78_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE24154.1"
                     /translation="MPAEDKYDYETGRMPDSVVEPLRREAKARGLWTPHLPESEGGLG
                     LDMVGTALVFSELGRSFIAPYVCNCDAPDEGNMHLLHLAANEEQKNLIYHPLRKGEIR
                     TGFAMTEPAPGAGADPQSLQTNAEKKGDKYVINGRKWYCTGANGAKYLIVMAKVGGSF
                     RRTTMFLVPTNAKGYTMVREIGVLGSHGPGGHCELLFEDVEVPENMILGRVGEGFRLS
                     QERLGPARLTHCMRWIGLARRAMETARTYALEREVFGSKLSDHEGIQWMFAESALEIE
                     QGFMLTLKAAHQLREGADARHIISMAKWAVSETLCKAIDRAIQITGSLGFSRDLKLEL
                     LYRDARAARIADGPSEVHKMVIARNLMNGKVQF"
     gene            1172..2131
                     /locus_tag="PLG78_13360"
     CDS             1172..2131
                     /locus_tag="PLG78_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011672004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNE24155.1"
                     /translation="MKSLSGGACQDNYLVDLTVEGGTEKGNHKLVMRTDKGASLFASL
                     SRVDEFHVCDLAYQAGVKTPRPMWLEENPAVLGAPFYFMERITGNANGRFVVKDASIA
                     NARKNLPAELATSLARIHSVKPASCKDEALKQKLGRISGEGKKAIALNSVIELRKETS
                     KLKEPHPALEWILNWLEDHAPETDEPVLIHGDFRTGNFMVSPAGLEGVMDWEFAHWGD
                     RHEDVSWLCMKDWRFGKLNKEAGGFTDRETFYSEYAKASGITLSPEKVRYWEVMGNAR
                     WACGSAGQAERHLSGMDKGIELASIGRRSSEMEFEAMRLIDAV"
     gene            2121..2507
                     /locus_tag="PLG78_13365"
     CDS             2121..2507
                     /locus_tag="PLG78_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HNE24156.1"
                     /translation="MQYRPDAPSLLEAIADFLMKDVLPAVKEHDALAYKTLVSWNMLG
                     VVSRELKSAEPLLNDEIARLLSHFKETRALPGSIGEKLELSRELNGRLAEEIRSRSGN
                     NAAQKELVKKALVENLSISNPRFGTE"
     gene            2507..3217
                     /locus_tag="PLG78_13370"
     CDS             2507..3217
                     /locus_tag="PLG78_13370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE24157.1"
                     /translation="MPRLYLVRHGRADSTGEVYDRLTDIGHRQAELLADYLVSSGVRP
                     DAVYHGTLQRQRETAEHLTRRRPALSSRFQNTETASLNEFTPEFWGAMSRKLSALDQT
                     FAHDLERYLAIRRKGGRRSMVLFLRLTEAIFESWATGMTPEGCESFDAFENRVLTFTE
                     KLLAHKESDTVVVFSSGTPLSIMIARLLGWDRSRSLDWMRYLQNTSLSVFLPGRAGLS
                     PAAINSLGHLPPDLHTLL"
     gene            complement(3125..4207)
                     /locus_tag="PLG78_13375"
     CDS             complement(3125..4207)
                     /locus_tag="PLG78_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE24158.1"
                     /translation="MAFFDPPSYLRPAWIQTLLPAIPFGVKRKHPVLASAEDIILQVD
                     GIRIHAALSRGTRRAIVILIHGWEGHIEAPYMLRTSQALSDHGFYVARLHLRDHGATH
                     HLNEELFNGSMLDEHFEAVRQIAKMFPGFSVYLGGFSLGGNFALRIAARNSRVKTPIV
                     NLKAVAAISPPLDPHRSTASMDEHRMLGRYLLWQWWRSLRTKEKLFPEKYNFSHLRKE
                     RSILNLTERLILAHSPFESLEEYFGTYTLGSEFFSRIKVPVSILTAEDDPIIPPEEYY
                     SLRVGPNVRIQMEKHGGHVGFLRSLRYDCAYVEFLVNAYEEQEDILRQGQRSKESKKS
                     VKVRRKVAKTVDGGRRKARTTRKKNR"
BASE COUNT         1138 a         1067 c         1147 g          939 t
ORIGIN      
        1 gcatccctgc agaagacaaa tacgattacg aaactgggcg aatgccggat tcagtagtag
       61 agccgcttcg ccgcgaggcg aaagctcgcg gcctgtggac cccgcatctt cctgaaagtg
      121 aagggggact ggggctggac atggtgggca cagctctcgt attctcagag ctcggaagat
      181 ctttcattgc tccatacgtc tgcaattgcg acgcaccgga tgaaggaaac atgcaccttc
      241 ttcatctggc ggcaaacgaa gagcaaaaga acttaatcta tcatcctctt cgcaaaggag
      301 agatcagaac cgggtttgcc atgacagaac cggcccctgg tgcaggtgcg gatcctcaaa
      361 gtctgcagac aaacgcagag aaaaagggag ataagtacgt catcaatggc cgcaagtggt
      421 actgcacagg agccaacggc gcgaaatact tgatcgtcat ggcaaaggtc ggaggaagct
      481 tccgcagaac taccatgttc ctcgttccca caaatgcgaa aggctatacg atggtgcgcg
      541 aaattggagt gcttggatct cacggccctg gcggccactg cgaacttctc tttgaagacg
      601 tagaagtgcc agaaaacatg atcctcgggc gagtgggaga aggtttcaga ctcagccagg
      661 aaagacttgg acccgcccgc ctaacacatt gtatgcgatg gattggtctt gcaaggcgcg
      721 ccatggagac cgcccggacc tatgctctgg aaagagaagt ctttggctct aaactttctg
      781 atcacgaagg aatccagtgg atgttcgcag aaagcgcgct agaaatcgaa cagggattca
      841 tgctcactct caaggcagct catcaattgc gcgaaggcgc ggatgctcgt cacatcatct
      901 ccatggcaaa atgggcagtc agtgaaacgc tttgcaaagc aatcgatcgc gccatccaga
      961 tcacaggctc tcttggattc agccgcgatc tgaaactgga actattgtat cgcgacgcaa
     1021 gagcagcgcg catcgcagac ggtccatcag aggtgcacaa gatggtcatc gcacgaaatc
     1081 tgatgaacgg gaaggtgcaa ttttgaatct ggaagaaaaa gtagcggact acctcacaag
     1141 aagactgggt gcgccggctc gcattgacaa gatgaaatcc ctttccggcg gcgcctgcca
     1201 ggacaactat ctggtggatc tgactgtgga aggcggaacg gagaaaggaa atcacaaact
     1261 ggtgatgcgc acggacaaag gtgcgtcttt gtttgcaagc ctttcgcgcg tcgatgagtt
     1321 tcacgtctgc gatcttgcct accaggcagg agtcaaaaca cccaggccca tgtggctcga
     1381 ggaaaacccc gccgtcctgg gagcaccttt ctatttcatg gaaagaatta cagggaatgc
     1441 aaatgggcga ttcgttgtaa aagacgcatc cattgcaaac gctagaaaaa atcttccggc
     1501 tgaactggca acgagccttg ccagaattca ttctgtaaaa cccgcttcct gcaaagacga
     1561 agcactcaaa cagaaactgg ggcgaatctc cggggaaggc aaaaaagcga tcgctctgaa
     1621 ttcagtgatt gaacttcgca aggaaacatc aaaactcaaa gagccacacc cggccctgga
     1681 atggattcta aactggctcg aagaccatgc accggaaacg gacgaacctg ttctcattca
     1741 cggcgatttt cgcacaggca acttcatggt ttctccggcc ggactggaag gagtcatgga
     1801 ctgggaattc gcccactggg gagacagaca tgaagacgtt agctggcttt gtatgaagga
     1861 ctggcgattt ggcaaactga acaaagaagc aggtggcttc acagaccgcg aaacattcta
     1921 ctctgaatat gcaaaggcat ccggtatcac actttcccct gagaaagtgc gttactggga
     1981 ggtgatgggg aatgcgcgct gggcgtgcgg atcggcgggg caggcagaga gacatctgtc
     2041 gggaatggat aaaggaatcg aacttgcctc cattggcaga agaagcagcg aaatggaatt
     2101 tgaagcaatg aggttgatcg atgcagtata gaccggacgc tccctctctt ctggaagcga
     2161 tcgcagactt cctgatgaaa gacgtattgc ctgcagtcaa agagcatgat gctcttgctt
     2221 acaaaactct ggtcagctgg aacatgctgg gagtggtttc cagggaactc aagtccgccg
     2281 aaccactgtt aaacgatgaa atcgcacgcc ttctttcgca tttcaaggaa acgcgagcac
     2341 tgcctggatc gatcggagaa aagctggaac tctccagaga gctcaatggc agactggcag
     2401 aagaaatcag atcgaggtcg ggaaacaacg cggcgcaaaa ggaactcgtg aaaaaagcgc
     2461 tcgttgagaa cctttccata tccaatccga gatttggaac agaatagtgc ccagactcta
     2521 ccttgtgcga catggtcgtg cagattctac cggggaagtg tatgatcgcc tgacggacat
     2581 tggccacagg caggcagagc tgcttgcgga ctatcttgtg tccagtggtg tgaggccgga
     2641 tgcagtgtat catggcactc tccagagaca gagggaaaca gcagagcatc taacaagaag
     2701 gcgcccggca ctcagcagtc gatttcaaaa cacagagact gcatctctca acgagtttac
     2761 cccggagttc tggggagcca tgtccaggaa actatccgct ctagaccaga ctttcgcgca
     2821 tgacctggaa cgttatctgg ccatacgcag aaaaggtgga aggcgatcga tggttctctt
     2881 cctacggttg acagaggcaa tcttcgaaag ttgggctact ggaatgactc cagaaggatg
     2941 cgaaagcttt gatgcatttg aaaaccgggt tctcaccttc acagaaaagc tgctggctca
     3001 taaggagtcc gatacagtgg ttgttttcag ttctggaaca cctctatcca tcatgattgc
     3061 gcgattgctc ggctgggata gatcgcgctc cctggattgg atgagatatc tgcagaatac
     3121 gtccctatct gtttttcttc ctggtcgtgc gggcctttct cctgccgcca tcaacagtct
     3181 tggccacctt ccgccggacc ttcacactct tctttgattc cttgcttcgc tggccctgac
     3241 gcaaaatgtc ttcctgttct tcgtatgcat tcaccaggaa ttcaacatac gcacaatcgt
     3301 agcgaagaga tctcaagaaa ccaacatgcc ctccgtgctt ttccatttga atgcgcacat
     3361 ttggaccaac gcgcaaactg taatattctt ccggaggaat gatgggatca tcctctgccg
     3421 tgaggataga gacgggcact ttgatccgcg agaaaaactc actgcccaga gtatatgtgc
     3481 caaaatactc ttcgagcgat tcgaatggtg aatgcgcgag gatcaatcgc tccgtaagat
     3541 tcagaatgga tctttctttt ctgagatgag agaaattgta tttttccggg aacagttttt
     3601 ccttcgtgcg aagagacctc caccattgcc agagcaaata acgtccaagc attctgtgtt
     3661 catccatgct cgccgtggat cgatgcggat caagaggtgg actgatggcc gccaccgctt
     3721 ttagatttac gattggagtc ttcacgcgag aattgcgtgc tgcaattcgc agtgcgaaat
     3781 tcccacccag ggaaaaccca cccagataaa ctgaaaagcc gggaaacatc tttgcaatct
     3841 gtctgactgc ttcaaaatgt tcgtccagca tactcccatt gaaaagttct tcgtttagat
     3901 gatgagtggc gccatgatct ctgaggtgaa gccgggcaac atagaagccg tgatcactga
     3961 gtgcctgcga agtgcgaagc atatagggcg cctcaatgtg gccctcccat ccatgaatga
     4021 gaatcacaat ggcccgtcgg gtaccgcgac tgagtgccgc gtgaatgcga atcccgtcta
     4081 cctgcagaat gatgtcttct gcggatgcga gcaccggatg ctttcgcttc actccgaacg
     4141 gaatcgcagg cagaagggtc tggatccagg cgggcctgag gtaagaaggc ggatcaaaga
     4201 acgccatggt gtagctctct attcggggct accaggtcga gctttttcaa cgaccctcgt
     4261 ccggggtttt gttttgtctt tacgaaagaa a
//
LOCUS       DAOQJL010000326         4287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12422_length_4287_cov_4.642486, whole genome shotgun sequence.
ACCESSION   DAOQJL010000326 DAOQJL010000000
VERSION     DAOQJL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_4287_cov_4.642486"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLG78_13380"
     CDS             <1..748
                     /locus_tag="PLG78_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24159.1"
                     /translation="ELEDVLDAIRKEPESPAQDRIASAGELGKKLIAEIQDGGTLFDK
                     IVNMKDVFRVKTLDASAEIAERCERIVEQEALAAGKAVAFEFENRAGVLRGDALVRVR
                     AMLGHMIRNAVVHGIETPDARKEKGKAIDGSIRLLMTHNDGQVRIAVEDDGAGLDQER
                     IARHAIEQGILTQEQADVASPAEGLRLIFRPGFSTAERVTESAGRGVGLDVVEEEARA
                     LGGNVRVETKRGTFTKFIVVLPESVLEKRD"
     gene            789..1154
                     /locus_tag="PLG78_13385"
     CDS             789..1154
                     /locus_tag="PLG78_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24160.1"
                     /translation="MKILVVDDSAMMRSVIKSTLASYGSGKQIEVVEAVNGQEALDKL
                     ASESIDLVLLDWQMPVVDGLSFVKQVRGSGKDVPIVMVTSVTDKEKVLEAGKAGVNTY
                     IEKPVRGSVLWENIQEFLH"
     gene            1168..1626
                     /locus_tag="PLG78_13390"
     CDS             1168..1626
                     /locus_tag="PLG78_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE24161.1"
                     /translation="MNTSHILAFSTCLKTTFRDMLSVDMTQGPIAEHVDYPVRADVSA
                     CITISSKAPACVVLAFDADSAMDAAERFTQTKSPFEDPMVGDTAREILNILVGSAQRE
                     ISEKFEFSLPISIQGRNHEVSCFRKGRNIFCDLHWGTGKKATLFLNLPAL"
     gene            1725..2339
                     /locus_tag="PLG78_13395"
     CDS             1725..2339
                     /locus_tag="PLG78_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNE24162.1"
                     /translation="MFSFIPKEEKYFRSLSELAARVRAGSEIFLRLFDDFGNHHKFAD
                     EIKSVEVQCDTIAASITSRLNTSFITPIDREDIFLLVKEVDDIMDAINGLAFRFDTYS
                     VKTPRSEAVEFARLIHQGAVELEHAFSMLEQNKNLGDHPKNINDIEKKADHLYRDAIR
                     RLFTEEKDPIEVIKWMSIFETLEDATDRCKDVAEALEGVVVKNK"
     gene            2345..3388
                     /locus_tag="PLG78_13400"
     CDS             2345..3388
                     /locus_tag="PLG78_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNE24163.1"
                     /translation="MNAVLVFVIIFVVIALAFDYINGFHDAANSIATVVSTRVLTPGR
                     AVLWAAFFNFVAFAIFGTHVAKTIGGDMVNIGLIQDDFAFRKLWVLLCGVIGAIIWNL
                     ITWYYGLPSSSSHALIGGYAGAAITANGGFSNGILISAGWIKTVSFIIISPLVGIILG
                     FLIMVAIMWIFRKFSPDRVDRLFRKLQLVSAALFSLGHGGNDAQKTIGIIAAALAASN
                     LVPDAAKNVPFWVVLICYAAIGLGTLSGGWRIVHTMGSRLTKLKPVGGFAAETAGAAA
                     MFMATYGGVPVSTTHTINGSIIGVGATRRLSAVRWGVARSVVWAWILTIPMSGGMAAL
                     LFTLVQLGYKLSA"
     gene            complement(3391..>4287)
                     /locus_tag="PLG78_13405"
     CDS             complement(3391..>4287)
                     /locus_tag="PLG78_13405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNE24164.1"
                     /translation="NFSSMGGRGLIHNGADDNASGTAAVLAAAKSWEEDVRKGTRKLP
                     ENTNVLFLNFDAEERGLFGSLAFVNHSPFPLSDAAVMVNLDMVGRYRPEKGMTVQGKD
                     SADPRLLEIFQAGFKETFPGNAKLKQISGGGGPSDHASFYMKGVPVAFLFTGYHLQYH
                     KPEDDFALLNIDGIDRSSRYAAFIANQVLKLEKPLQFKRASEEGQEGQMEFRLRLGIM
                     PGSYEGGTEGLLVGGVHKGAPVEKTGIQEGDVIISLGGKTIHDVQDLMEFLNTASADQ
                     SYTIVFMRNKERITKQTRLMSE"
BASE COUNT         1039 a         1035 c         1134 g         1079 t
ORIGIN      
        1 cgaactggag gatgtgctcg atgcaattcg caaagaacca gaatctccgg cgcaggatag
       61 aattgcatct gcgggggaac ttggaaagaa actcattgca gagattcagg atggcgggac
      121 gttgtttgac aagatcgtaa acatgaagga cgtgtttaga gtcaagactc tcgatgcatc
      181 tgcagagatt gcagaaagat gtgaaagaat tgtagagcag gaagcactcg ccgcgggcaa
      241 agctgttgcg tttgagtttg agaatcgtgc gggagttctc cgtggagatg ctctggttcg
      301 cgttcgcgcg atgctgggtc acatgattcg aaacgctgta gttcatggaa ttgaaacgcc
      361 cgacgcgcga aaagagaaag gaaaagcaat cgatggttcc atacgccttc tgatgactca
      421 caatgacggc caggttcgaa ttgcggtgga agatgacggg gcgggcctgg atcaggagag
      481 aatcgcccgt cacgcgatcg aacagggaat cttgacgcaa gaacaggcgg acgttgcatc
      541 acctgcagag gggttgagat tgattttccg tccgggcttc agcaccgcgg agcgagttac
      601 agagagtgcg ggccgtggag tgggcctgga tgtagtggaa gaagaagcca gagcgctcgg
      661 aggcaatgtt cgagtagaga ccaaaagagg aacattcacc aaattcatcg tggttctgcc
      721 cgagtctgtt ctggagaagc gggattgaat tgccggcata ccccggcgtc tcgaatctga
      781 catctgtaat gaagatactt gtagtggacg attctgcgat gatgcgaagc gtgattaaaa
      841 gcacgctggc aagctatggc agcggtaaac agattgaagt ggtggaagcg gtcaacgggc
      901 aagaagccct ggacaaactc gcaagcgaaa gcatcgatct ggttctcctg gactggcaga
      961 tgcctgtggt agatggtctt tcttttgtga agcaggtgcg aggctccggg aaagacgttc
     1021 caatcgtcat ggtaacctct gtcaccgaca aagaaaaagt attggaagca gggaaggccg
     1081 gtgtaaacac ttacatcgaa aaaccagttc gaggcagcgt gctctgggag aacattcagg
     1141 aatttctgca ttaagaggga tggaaacgtg aacacttcgc acatactggc tttctctaca
     1201 tgtctcaaaa ctacgtttcg cgacatgctt tccgtagaca tgacacaggg gcccattgcc
     1261 gaacacgtag attatcccgt tcgcgcggac gtgagcgcat gcatcaccat ttcatcgaag
     1321 gcacccgcgt gcgtagtgct tgcgtttgac gcagacagcg ccatggatgc ggcagagcgt
     1381 ttcactcaga ctaaatctcc ttttgaagac ccgatggtcg gagacactgc gcgcgaaatt
     1441 ctaaacatcc tcgtcggatc cgctcagcgc gaaatctcag aaaaattcga attctccctt
     1501 cctatttcca ttcagggccg aaaccacgaa gtgagctgct ttcgcaaggg tagaaacatt
     1561 ttttgtgacc tgcactgggg cacgggcaag aaagcgactc tttttctgaa cctgccagca
     1621 ctctaaatca cacaaaaaaa acagatcgag gctgtgaaat cctgcgggac agcgcggaag
     1681 tgcaccccag tcttgtgatt acaaaactgt gtcaggggtt tgtcatgttc agcttcattc
     1741 caaaggaaga aaaatacttc cggtccctat cagaacttgc agcgagggtg cgagcgggca
     1801 gtgaaatctt ccttcgcctg tttgacgatt tcggaaatca tcacaagttc gcagacgaaa
     1861 tcaaaagcgt agaagttcag tgcgatacaa ttgccgcaag cattacatcc agactcaaca
     1921 caagtttcat cactccgatt gatcgcgaag acatctttct actggtcaaa gaagtagacg
     1981 atatcatgga cgcgatcaat ggcctggcgt ttcgttttga tacatacagt gttaagactc
     2041 ctcgatcgga ggcagttgaa tttgcaagat tgattcacca gggtgcagtg gagcttgaac
     2101 acgctttctc catgctcgaa caaaataaaa acctgggaga tcatccgaag aacatcaacg
     2161 acatcgaaaa gaaagcagac catctatacc gcgatgcgat ccgcagactc ttcacagagg
     2221 aaaaagatcc gatcgaagtc atcaagtgga tgagcatctt tgaaactctc gaagatgcaa
     2281 ccgaccgctg caaagatgtg gcagaggcgc tcgaaggcgt cgttgtaaag aacaaataaa
     2341 gtttatgaac gcagttctgg ttttcgtaat catattcgtg gtcattgctc ttgcttttga
     2401 ctacatcaat ggatttcacg atgcagcaaa ttcaatcgcc actgttgttt ccacgcgtgt
     2461 cctgactcct ggtcgcgctg tactgtgggc tgcttttttt aatttcgtcg cctttgccat
     2521 ttttggaaca cacgttgcca agacaatcgg cggagacatg gtgaatattg gactcatcca
     2581 ggacgatttc gcgtttagaa agctatgggt tttgctctgc ggcgtgattg gagcgattat
     2641 ctggaatttg atcacctggt attatggtct gccgtccagt tcttctcatg ctttgattgg
     2701 cgggtatgcg ggtgcggcga tcacggcgaa cggcggcttt tccaatggga ttctgatttc
     2761 tgcaggctgg attaagacgg tttctttcat catcatttct cctctcgtcg ggatcattct
     2821 gggttttctg atcatggtcg cgatcatgtg gatctttcgc aaattcagtc ctgatcgagt
     2881 ggatcgattg tttcgaaaat tgcagcttgt gtctgccgct ctcttcagcc tcggacacgg
     2941 cggaaacgac gcacaaaaaa caatcggtat catcgccgct gctctggcag cttccaatct
     3001 agttccggat gcagcgaaga atgttccttt ctgggtggtt ctcatatgct acgctgccat
     3061 agggcttgga actctatctg gcggatggcg gattgttcat accatgggtt cacgtctgac
     3121 caagctcaag ccagtgggtg gatttgctgc agaaactgca ggcgcggctg ccatgtttat
     3181 ggcaacctac ggtggcgttc cagtcagcac aacccacacg atcaatggtt ccatcattgg
     3241 cgtgggcgcc accaggcgac tttctgccgt gcggtgggga gtggcacgca gcgtagtgtg
     3301 ggcctggatt cttacgatcc ccatgtcagg tggaatggca gcactgctct ttacactggt
     3361 ccagctgggc tacaaacttt ctgcctgacg ttattcgctc atgaggcgcg tctgttttgt
     3421 gatgcgctct ttgtttctca tgaatacaat cgtataggat tggtccgcgg aagcagtatt
     3481 caggaactcc attagatcct ggacatcgtg gatggtcttg ccccccaggg atatgatcac
     3541 atccccttcc tgaatccccg tcttctctac gggtgcaccc ttgtgaactc ctcccacaag
     3601 aagtccttct gtaccgcctt cgtagcttcc gggcatgatt ccaagtcgga gcctgaattc
     3661 catctgccct tcctggcctt cttctgacgc tcgtttgaac tgaagcggtt tttccagttt
     3721 cagtacttga ttcgcgatga atgccgcata acggctggat ctatcaattc catcaatgtt
     3781 cagaagtgca aaatcgtcct cgggtttgtg gtattgcagg tggtatccgg tgaacagaaa
     3841 tgcaacaggg actcctttca tatagaaaga agcgtgatcg cttggtccgc ctcctccgga
     3901 gatttgtttt agcttcgcgt ttcctggaaa ggtttctttg aaaccagcct ggaatatttc
     3961 cagcagccgt ggatctgcac tgtcctttcc ctgcacggtc atccctttct ccggtctgta
     4021 tctgcccacc atgtccaggt tcaccatcac ggccgcgtcc gagagtggga aaggagaatg
     4081 gtttacaaat gcaagtgatc caaagagacc gcgctcttct gcatcaaagt tgagaaacaa
     4141 tacgttcgta ttttctggaa gctttcgcgt cccctttcgc acatcttcct cccaggattt
     4201 tgccgcggcc agcactgctg ccgtgccgga tgcattgtca tctgcgccgt tatgaatgag
     4261 tccgcgcccg cccatgctgg aaaagtt
//
LOCUS       DAOQJL010000327         4287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12423_length_4287_cov_3.983223, whole genome shotgun sequence.
ACCESSION   DAOQJL010000327 DAOQJL010000000
VERSION     DAOQJL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12423_length_4287_cov_3.983223"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLG78_13410"
     CDS             complement(<1..664)
                     /locus_tag="PLG78_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24165.1"
                     /translation="MKQASIVLLYLFAACSTVQEVQVPSASRTEFPELAVAPSVRTGV
                     SDKREPGVHSPDGKLKFFLSRGAPLANVNRSMQETALLRVLRSIRFRDVDYVTLDDPM
                     GKPVALDGRLSLTFEAGKTTNEFTIAARLKEEEEGVKIQFRIEPAAAPEQLEISHTEG
                     ISILRSPSSLRVASDTLRIEEIHNMLTDSIMGTLDVQSSSAETVVSLKGSSEISVGPA
                     PVS"
     gene            complement(692..1108)
                     /locus_tag="PLG78_13415"
     CDS             complement(692..1108)
                     /locus_tag="PLG78_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24166.1"
                     /translation="MIHAVGVKKIMAKAVNSKTTTKEPLSAEQMEIFFNLLTDYTDKV
                     SLINSVYESDLETAFQAMEGAVEEVLQWDTSSFSPDLRRTLLSYLTFHRETVWEIIQE
                     ARSLLMSDRREYVKRLVNHHKEFIKWLSRMERNFAA"
     gene            1228..2181
                     /locus_tag="PLG78_13420"
     CDS             1228..2181
                     /locus_tag="PLG78_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24167.1"
                     /translation="MIVTLLGVRGSIATPLRSQEYQGKVRQILEKAIRENINESRIDS
                     FISELPPDLRYVIGGETTCCTVQIPGVPLPVIIDCGTALRHLGDELMAGPCGKGQGEL
                     HILVTHTHWDHIQGLPFFKPAYIPGNKLHFYSPLDDLQDRLVYQTTDRFFPMPFMATA
                     SEKHFHKLTGPIDLGGVQVDHHPLKHPGGCFAYKFSKDGHAFIFATDAEFTGTDLEHP
                     EEYKPFFYQADLVVMDAQYTLDESFAKFDWGHTSYTMAVNCAAQWKVKNLVLTHHEPA
                     YSDAKVFENYESAIEHRNIMRLEFPRVHLAREGLSFRIGPE"
     gene            2178..2618
                     /locus_tag="PLG78_13425"
     CDS             2178..2618
                     /locus_tag="PLG78_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24168.1"
                     /translation="MNRAFTLTAGFILFAAFGSVLPAIYSVYTRDLRAMDHAKGSQVF
                     VTLAKAQTRRSPTGDRFYYSIEFKWDGTKYRIPVEVTSEFYHLSKEGQNMEAQIFFNM
                     GRPYVRLPGNHIHPAGRPGLFYLGAAGLLAGILLLASSVLARSR"
     gene            2618..3406
                     /locus_tag="PLG78_13430"
     CDS             2618..3406
                     /locus_tag="PLG78_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNE24169.1"
                     /translation="MDLAISPCPNDTFIFCHLMRAMPELQLVFADVEELNQRALFEKK
                     HDLSKLSFYAILRTKGYTLLDCGGAMGQGCGPILLYGKKSNGKISDLKRILTPGKYTT
                     AALLLDLFLAENHPDPASVERVPVRYDKILPALGEEQAGVIIHEERFTYPSYGAVKAQ
                     DLGEWWEKSTGLPIPLGGIVIRNDLLDRKSEIESKIRDSLRAAWKDPESVKDFVKCYS
                     QSLEDSVIQSHIDLYVNRYTESLGEDGMRALDVLRQKAERAGLL"
     gene            3571..3753
                     /locus_tag="PLG78_13435"
     CDS             3571..3753
                     /locus_tag="PLG78_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24170.1"
                     /translation="MKSIPASSVTQTDSNGEATLANSEGQSIRAYYGIGKVVVTVMAD
                     DADPIYFELEVLPREN"
     gene            complement(3791..>4287)
                     /locus_tag="PLG78_13440"
     CDS             complement(3791..>4287)
                     /locus_tag="PLG78_13440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE24171.1"
                     /translation="WKRQRLAELEFDLPASLHFVAVDFEAGESWKDELLKAGFDKSKP
                     TIFASTGVSMYLSRDANAETFGQIARLAPGSIFVTTFMLALELLNAQERSIMDFVMNK
                     ARESGTPFLSLFAPEEIVSLAKSSGFRTAEYVSASEIFDRYFAGRSDGLRAGDAEAFL
                     VAST"
BASE COUNT         1069 a         1116 c         1046 g         1056 t
ORIGIN      
        1 tagaaacagg ggccggacca acactgatct cagaggatcc tttcagggat acaacggtct
       61 cagcggatga cgactgcacg tcgagagtgc ccatgatact atccgtgagc atgttgtgga
      121 tttcttctat gcgaagagta tcgcttgcaa cgcgcagact cgacggagaa cgcaggatgg
      181 agatgccttc tgtatgcgaa atttccaatt gctccggagc ggcggcaggt tcaatgcgaa
      241 attgaatctt cactccttcc tcttcttctt tcagacgagc agcaattgta aattcgtttg
      301 tggtcttgcc tgcttcgaat gtaagagaga gtcttccatc gagtgcgacg ggcttgccca
      361 ttggatcgtc cagggtgacg tagtccacat cacggaatcg aatggaacgc agaacgcgca
      421 gaagcgctgt ctcctgcatg cttcgattca cgtttgcaag aggagctcct cttgatagga
      481 agaacttgag tttaccatcc ggagaatgca ctccgggttc acgcttgtct gagactccag
      541 tgcgcacaga tggcgccact gcaagttcag gaaattcggt gcgcgaggca gaagggacct
      601 gcacctcctg gacagtggag caggcagcga aaagatatag aagtacgatt gaggcttgtt
      661 tcatattcgg gctgcgcgcc cgatctatgc atcaggcggc gaagtttctc tccattcggg
      721 acagccactt tatgaattcc ttgtgatggt ttacaagcct ttttacgtat tccctccgat
      781 cggacataag aagacttctg gcttcctgga taatttccca gacggtctcc cggtggaatg
      841 tcagatagct cagtagagta cgtctgagat caggagagaa ggaagatgtg tcccactgga
      901 gcacttcctc cactgctcct tccatggcct ggaatgcagt ctccaaatcc gactcgtaca
      961 cactgttgat aaggctgacc ttatccgtgt aatccgtcag taagttgaaa aatatttcca
     1021 tctgctcggc agacaggggt tctttcgtag tggtctttga gttcacggct tttgccataa
     1081 tttttttgac gcctacggcg tgaatcattt attgtgcttt ttcagatcaa agttccaggg
     1141 ccgcggaagc ggcgtgcaga ttcggaaccg ggatcgctgg tcctaccagc ccctgaaaga
     1201 tgtctgagac tgcggggttt cgcgccaatg attgttactc tacttggagt acggggttcc
     1261 attgccactc cgttgcgctc tcaagaatat cagggcaaag ttcgccagat acttgaaaaa
     1321 gcaatccgcg aaaacataaa cgagtcccgc attgattcgt tcatttccga attaccgcca
     1381 gaccttcgat acgtgatcgg cggagaaacc acatgctgca ccgtgcaaat tccaggagtt
     1441 ccacttcctg tcatcatcga ttgtggcact gctctgcgtc atcttggtga cgaactgatg
     1501 gcgggcccgt gtgggaaagg ccagggcgaa cttcacattc ttgtaactca cactcactgg
     1561 gatcatatcc agggacttcc atttttcaag cctgcgtaca ttcctggaaa taaactgcat
     1621 ttctattctc ccctggatga tttgcaagat agactcgtct atcagaccac agatcgattt
     1681 ttcccgatgc catttatggc caccgcatct gaaaaacatt ttcacaagct cacaggaccc
     1741 attgacctgg gcggagtgca agtggaccat catcctctca agcatcctgg cggatgcttc
     1801 gcgtacaaat tttcaaaaga tggccacgca ttcattttcg caacagacgc ggaattcaca
     1861 ggaacagatc tggagcaccc cgaagaatac aaacccttct tctatcaggc ggatctggtc
     1921 gtcatggacg cgcagtacac tctggatgag tctttcgcaa aattcgactg gggccacaca
     1981 agctacacga tggcagtcaa ttgtgctgct cagtggaaag tgaaaaacct ggtgctcact
     2041 caccacgaac cagcctattc agacgcaaaa gtttttgaga attacgaaag cgccattgaa
     2101 catcgcaaca tcatgcggct tgaatttccg cgcgttcacc ttgcccggga aggattgtcc
     2161 ttccgcattg gcccggaatg aaccgtgctt tcactctgac tgctggattc attctgtttg
     2221 cggcgttcgg aagcgtcctt cccgccattt attccgtgta tacaagagat ttgcgtgcga
     2281 tggatcatgc gaagggctct caggtgtttg tgactctcgc gaaagcccag acaaggcgca
     2341 gccctacggg agatcgcttc tactattcca tcgaattcaa atgggacgga acaaaataca
     2401 gaattcctgt agaagtcact tccgagtttt atcatctttc caaagaggga caaaacatgg
     2461 aagcgcagat cttctttaat atggggcgcc cctatgtgcg tctcccgggc aatcacatcc
     2521 atcccgcagg aagaccgggt ctgttctatc tgggggctgc cggtttattg gctggaattt
     2581 tgctccttgc gtcttccgtt ctggcgaggt ccaggtgatg gatcttgcca tttctccctg
     2641 ccccaacgac actttcattt tctgtcactt gatgcgtgcc atgccagagc tgcagcttgt
     2701 gtttgcagat gtagaggagt tgaaccagcg cgcactcttt gaaaagaaac atgatctttc
     2761 taaactatcg ttctatgcaa ttctccgcac aaaaggatac acattgcttg attgcggtgg
     2821 tgcgatgggc cagggatgcg gacccattct tttgtatgga aagaaatcaa acggaaagat
     2881 atctgacctc aaacgaatcc tgactccggg caaatacaca acggctgctc ttcttctcga
     2941 tctgttcctt gctgaaaatc atccagaccc tgcgtccgta gagagagtcc ctgtaagata
     3001 tgacaaaata ctcccggccc tcggagaaga acaggcaggc gtgatcatcc acgaagaacg
     3061 ttttacgtat ccgtcgtatg gcgcggtcaa agcccaggat ctgggagaat ggtgggaaaa
     3121 atccacggga cttcccattc ctctgggcgg cattgtaatt cgaaacgatc ttctagatcg
     3181 caaatccgaa atagaatcaa agattcgaga cagtctgcgc gcagcgtgga aggatccaga
     3241 gtctgtcaaa gacttcgtga aatgctattc gcagtctcta gaagattcag taatccaatc
     3301 tcacatagat ctctatgtga accgttatac ggaatctctg ggagaagacg gaatgcgtgc
     3361 tctggatgtg ctaagacaga aggcggagag agccggattg ctctaacagc tggccgcatc
     3421 atcccgattt cgtgcagcgc cccgcaagac cgtcatggaa catcgcctaa ttcctgttac
     3481 atcccggagg aatttcatcc gtccccccat ccaggtttac tacacgaaac aaaggttgcg
     3541 tttgccgagg ggtaacgcaa cgttcgcgcc gtgaaatcaa ttcctgcgtc ctccgtcact
     3601 cagacagact caaacggaga ggccacgctc gcaaattctg aaggccaaag cattcgcgct
     3661 tactacggca ttgggaaagt agtggtcact gtcatggcag atgacgcaga tccgatctat
     3721 ttcgaattgg aagtgctgcc gcgcgagaat tgagtttcgc gtttcctgac gtccgcggta
     3781 ccacgacaga tcaagtgcta gccacgagga aagcctccgc gtctccagcc ctgagcccat
     3841 ccgaacggcc tgcaaaatag cgatcgaaaa tttcactcgc agacacgtac tccgccgttc
     3901 taaaaccact gctcttcgcc agagatacaa tttcttcggg agcaaacaaa ctcaagaatg
     3961 gtgtgccgct ctccctggct ttattcataa caaagtccat aatggatctc tcctgggcat
     4021 tcaaaagctc aagggcaagc atgaaggttg tcacaaaaat cgacccagga gccaggcgcg
     4081 cgatctggcc aaatgtttcc gcatttgcat ctctggacaa atacatagaa acaccggtag
     4141 acgcaaaaat cgtcggttta cttttatcga accctgcctt gagtagctca tccttccacg
     4201 attctcccgc ttcaaaatcg acagcaacga aatgcagcga cgcaggcagg tcgaactcga
     4261 gctctgcgag tctttgtctc ttccaca
//
LOCUS       DAOQJL010000328         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12494_length_4271_cov_3.806214, whole genome shotgun sequence.
ACCESSION   DAOQJL010000328 DAOQJL010000000
VERSION     DAOQJL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12494_length_4271_cov_3.806214"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLG78_13445"
     CDS             complement(<1..349)
                     /locus_tag="PLG78_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24172.1"
                     /translation="MGDTFDFEFIRHLVKQAFHILNHEYFRAKLVNLDRVPAKTPGRP
                     RIFFSNHSGMAFPWDAIVFNTLFWESRGLANEDLPRPLVAPMLLESKLMSPFMIENLW
                     QRGGCLAASMENFE"
     gene            complement(405..1193)
                     /gene="folP"
                     /locus_tag="PLG78_13450"
     CDS             complement(405..1193)
                     /gene="folP"
                     /locus_tag="PLG78_13450"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE24173.1"
                     /translation="MRILGIVNITDDSFSDGGKFLSSRSAIDHAVHLSRGGADIVDLG
                     AASSNPEASRVPPAVEIERLAPVIRALRKGSVEVSVDSFSTEVQRFALSEGVHMLNDI
                     HGFADPDLYKELGKSSAMLVVMHSVQKEGVATKANTPAQEIVGRILNFFDERVETLLR
                     FVSRDRLILDPGLGFFLGTDPACSFAAIRAIPEIKSRYGLPVLISASRKSFLGAVTGR
                     AVLERSAATLACELEARRLGADYIRTHDPAALLDGCKIQDQIYS"
     gene            1255..1644
                     /locus_tag="PLG78_13455"
     CDS             1255..1644
                     /locus_tag="PLG78_13455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNE24174.1"
                     /translation="MSAFSSILNGTEPCEKILEDERYLAFMEKKPLKEGHVVVLPKRE
                     TDFIFDLSAEELSGLFVFAQRVAQAIRRAIPCKKVGVAVIGIEVRHAHVHLVPLDSGA
                     ELNFALPRREFTPDQFRVTADKIRAQI"
     gene            1685..2437
                     /locus_tag="PLG78_13460"
     CDS             1685..2437
                     /locus_tag="PLG78_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNE24175.1"
                     /translation="MILMPVSSTDFDPSEASIAWKVLNDRGIAVTFATPDGKPGSADH
                     RMVTGDGLGVWKRILMARKDAVAAYHEMTASSAFQKPLLYSEARASDFKGIILPGGHA
                     PGMRPYLESQDLMKVIGDFMEAAKPVGAICHGVLLAARSKGQNGKSVLYGRKVTCLLR
                     SQEMAAYRLTSLWLGNYYRTYPETTTQDEVTSFLKDPSDFSEGPTPMFRDSEENRKPG
                     FAVVDGNLITARWPGDGYTFAHGFLKVAGMGD"
     gene            complement(2477..>4271)
                     /locus_tag="PLG78_13465"
     CDS             complement(2477..>4271)
                     /locus_tag="PLG78_13465"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24176.1"
                     /translation="SLAFRAGIIRDRDRVFINGNLIGSTGEWDASAPQAFDRSRLYEV
                     KPDVFRTGDNLILIQIQGTPELPPAIRFGPLSLGTIQDALTRQASTAYMHGALTVVYL
                     TIASYFLFLFLKRSEERDNLYFGLFTILVAIFTFLQSELKYQIQLSFMTFKKIELIAQ
                     FASVPAYFAFIQAFFPERRERDRGVLKVIGLSGYGIIALACILVAATSNLSIWIFVRN
                     YGVTTGCILLVVGGAGLVYNRLRAFERDAFYFTGGLIVLAGGIIFAYLSETGILHLPN
                     VLDGFLLIFVIINAMTLANRILRLTAQVEEMNADLERRVQRRTEELGESLEKVRALKE
                     SQDADYFLTTLLLSPLSGAKLTESGMTPSILIKQKKSFDFRKWRAEIGGDICVVDELP
                     LKGRRYAIFMNGDAMGKSMQGAGGALVLGTVFRSLITRTQKAERFQNQTPQEWLRDCF
                     LELQTVFVTFDGSMLVSAVFGLIDLESREMYHVNAEHPTSVLLRNGKASFVPTLHPVM
                     KIGVRGMEDKMQVEQLLLQSGDILIAGSDGRDDVLLGYEPDGTRIINADESLFLRIVE
                     NCGGSLGKIYESIQASGGITDDLSLLAIEVS"
BASE COUNT         1064 a         1071 c         1102 g         1034 t
ORIGIN      
        1 tctcgaaatt ttccatactg gccgcgaggc acccgcctct ctgccagagg ttttctatca
       61 tgaaaggaga catgagtttg ctttccagaa gcatgggcgc aaccagtggg cgagggagat
      121 cttcattggc aagcccgcgc gattcccaga acaaagtatt gaatacaatt gcatcccacg
      181 gaaatgccat tccggaatga tttgaaaaaa agattctggg tctacccggc gttttcgcag
      241 gcacccgatc caggttcacc agttttgcgc gaaagtactc atgattgaga atatgaaacg
      301 cctgcttgac caggtgccgg atgaattcga aatcgaaagt gtcccccata acacacgaag
      361 actaacacag ttgcacgggg gatggtatga caaatgtcag aaagctagga ataaatttga
      421 tcctgtattt tgcagccatc caggagtgct gctgggtcat gcgttcgtat gtagtctgca
      481 cccaggcgcc tggcttccaa ctcgcaggca agagtggccg cggatctttc caggactgct
      541 ctacctgtaa ctgcacccag aaatgatttc ctggaagcgg aaatgagtac aggcagtccg
      601 tacctggatt tgatttcagg gatggcgcga atcgcagcga agctacaggc ggggtctgtt
      661 ccaagaaaga atccaagccc ggggtccagg atgagcctgt ctcgtgatac gaaacgaaga
      721 agtgtttcta ctctttcgtc gaagaaattt aaaattcgtc ctacgatttc ctgggccgga
      781 gtgtttgctt ttgtagccac gccttccttt tgcactgagt gcatgactac gagcatcgca
      841 cttgattttc caagctcttt gtagaggtca gggtccgcga agccatggat gtcattgagc
      901 atgtgcactc cttccgacag cgcaaatctc tgcacttctg tagagaacga gtctacagac
      961 acttccacgg agccttttct caaagctcga atgaccggtg ccagtctctc tatttctacg
     1021 gcaggtggaa cacgggaagc ttcggggttg ctcgatgccg cgcccagatc gacgatgtct
     1081 gcgccgccgc gcgacaggtg caccgcgtga tctatagcag atcggctgga tagaaatttg
     1141 ccaccgtcag agaaagaatc gtcggttatg tttactatgc ccaggattcg cacgctcgat
     1201 tttcaccggg cgttgtgtgc ttgcaagccc gatcggaagg tctcgaatga cgcaatgagc
     1261 gccttttcat ccattctgaa cggcacagag ccctgcgaga agatcctgga agatgaacgt
     1321 tatctggcat tcatggaaaa gaaacccctc aaagaaggcc atgtggtagt gctgccaaag
     1381 agagagactg actttatatt tgatctttct gcagaagaac tgagcgggct gtttgttttc
     1441 gcacagcgcg tggctcaggc gattcgtcgt gcgattccct gtaagaaagt aggtgttgca
     1501 gtcattggga tagaagtgag gcatgcgcat gttcacctgg ttcctctgga ttcaggtgcg
     1561 gagttgaatt tcgcgcttcc caggcgcgaa tttactccgg atcagtttcg agtgaccgca
     1621 gacaaaatcc gcgcacaaat ttgaaaatcg catgactgat cgaagagggg ggcctcattg
     1681 aggcatgatt ctcatgccag tgtccagcac cgattttgac ccttctgaag caagcattgc
     1741 ctggaaagtg ctcaacgatc gcggaatcgc agttacattc gccactccag acggcaagcc
     1801 gggaagtgca gatcatcgca tggtcacagg ggatgggctt ggagtatgga agcggattct
     1861 gatggctcgc aaagatgctg tggcagccta tcatgaaatg acggcgtcct ccgccttcca
     1921 gaagcctctt ctctattcag aagcgcgtgc gtctgatttc aaaggaatca ttttaccagg
     1981 tggacacgct ccgggaatga ggccgtatct ggagtctcag gacttgatga aggtcattgg
     2041 cgattttatg gaagcggcca aaccagtcgg agccatttgt catggcgtac ttctggccgc
     2101 tcgttcgaaa ggacagaacg gcaagtctgt tctatacggc aggaaggtaa cgtgtctcct
     2161 tcgatcgcag gaaatggccg cctatcgttt gaccagtttg tggcttggaa actactatag
     2221 aacctacccg gaaacaacca ctcaggatga agtcaccagt ttcttaaaag atccttctga
     2281 tttttcagaa ggccccacac ccatgttcag agacagcgag gagaatcgta aacctggctt
     2341 tgcggttgta gatggaaacc tcatcacagc gcgctggccc ggagatggct atacgttcgc
     2401 tcacggtttt ctaaaagtcg caggcatggg cgactaacgt tccggtaaaa aagattcgtg
     2461 gaactgagta agagagtcag ctcacttcaa tggccagtag actcaaatcg tctgtgatgc
     2521 ctccgcttgc ctgaatggat tcgtaaattt ttcccaggct tccgccgcag ttttccacga
     2581 tgcgcaaaaa gagggactcg tctgcattga tgatgcgcgt gccgtccggt tcgtagccca
     2641 gaagaacatc atctctgccg tcactgcccg caattagaat gtctccgctc tgaagcaaga
     2701 gttgctccac ttgcattttg tcttccatcc ctcgcacacc gatcttcatg acagggtgga
     2761 gtgttggaac aaaggatgct ttcccgtttc gcaacagtac agaggtggga tgctcagcat
     2821 ttacgtgata catttccctg ctttccagat caatgagacc aaagactgca gaaacaagca
     2881 tgcttccatc aaacgtgacg aagacagttt gaagctccag aaagcaatct ctcagccact
     2941 cctgtggagt ctggttctgg aatctttctg ccttctgcgt tcttgtgatg aggctacgaa
     3001 acaccgttcc aagcaccaga gctccaccgg cgccctgcat ggatttgccc attgcatcac
     3061 cgttcatgaa aatcgcataa cgtcttcctt tgagaggaag ttcatccact acgcaaatat
     3121 ccccgccaat ctcagcgcgc cattttctaa agtcgaagct cttcttttgt ttgatcagga
     3181 tgctgggtgt cattccgctt tcggtaagtt ttgcgcctga aagaggagag agtaggagtg
     3241 tagtcaggaa atagtctgcg tcctggcttt ctttcaatgc tcgcactttt tccagagatt
     3301 ctccgagctc ttccgtgcgc ctctgaacgc gtctctccag atccgcgttc atttcttcca
     3361 cctgtgctgt aaggcgaaga atgcgatttg caagagtcat ggcgtttatg attacgaaga
     3421 taagcaggaa gccatcgagc acattgggaa ggtgaaggat tccggtttct gacaaatagg
     3481 cgaatatgat cccgcccgcc agcacgatca gtccgcctgt gaagtagaac gcgtcgcgtt
     3541 cgaacgcgcg cagtctattg tatacgaggc ccgcgccacc caccaccagt agaatgcatc
     3601 cggttgtaac tccgtaattt cgcacgaaaa tccatatgct caggttcgaa gtcgcagcca
     3661 caaggatgca ggccagtgcg atgatcccgt agcccgaaag accgatgact ttcaaaacac
     3721 ctctgtccct ctccctccgt tccgggaaga acgcctggat gaaagcaaag taggccggca
     3781 cagaagcgaa ctgtgcgatc aattctatct tcttgaatgt catgaaagac agttggatct
     3841 gatacttaag ttcgctctga agaaatgtaa agatggctac gagaatcgta aataaaccaa
     3901 agtaaaggtt gtccctctct tcgctgcgct tcagaaacaa gaaaagaaaa tacgaagcga
     3961 tggtcagata gacgacagtg agagctccgt gcatgtaggc agtacttgcc tgacgagtca
     4021 aagcgtcctg aatcgttcca agggagagtg gaccgaaccg aatggcagga gggagttctg
     4081 gagttccctg gatttgaatt aagatcagat tgtcaccggt gcgaaataca tccggcttca
     4141 cttcgtacag acgacttctg tcaaatgcct gaggagcgct cgcgtcccat tctcctgtgc
     4201 tgccaatgag gttgccattg ataaacacgc gatcccggtc gcgaatgatt ccagcgcgaa
     4261 aggctagaga c
//
LOCUS       DAOQJL010000329         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12555_length_4260_cov_3.847087, whole genome shotgun sequence.
ACCESSION   DAOQJL010000329 DAOQJL010000000
VERSION     DAOQJL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_4260_cov_3.847087"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLG78_13470"
     CDS             <1..194
                     /locus_tag="PLG78_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24177.1"
                     /translation="GCGGEAMGPVQIGNKTLQVPGDVACAGEETRIYDQRELLMKDLG
                     IKPEDLGIQGNVRFKNEKQ"
     gene            224..1381
                     /locus_tag="PLG78_13475"
     CDS             224..1381
                     /locus_tag="PLG78_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE24178.1"
                     /translation="MKSTQIGKDRREGQFAQLLEASLKNQTKVEAGSYDLVTVSDITN
                     KDFVFVQSPRGTGLIPREELLDHHGKIRVSRGEKIPAFYRSTENGEMMFTTQPSGRAK
                     MDIVRTAIENKIPMTGRILKKIKGGFEVVIGEVNAFCPASHMDGNPDVKSEVTFLITE
                     AKGDRVIASNRAFRDIEKKRQKDVLMQSLQPGDVVEGTVANLADFGAFIDIGGMEGLV
                     PLSEIAHQRVTHPSQVLKSGQSVRVKILQIDWKDDRLTLSLRALLENPWQGQLPWKEG
                     DIVEGKVESIKPFGVFVKLEGNFTGLIPAGESGVARGQPFEKFFQKGQTLRVMVHTID
                     REREKISLSVRGVAAHDQEQEFAEYLKVQGPQKEELSSFGQQLRDAMNKKK"
     gene            1430..4135
                     /locus_tag="PLG78_13480"
     CDS             1430..4135
                     /locus_tag="PLG78_13480"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE24179.1"
                     /translation="MNEPLSKPHALSTDQILELVTSSRTGLSVAEADRRLALWGPNEI
                     PTQKRSSWIRILAQQLKNVLIIILLLATVLSAVLGHSTEAAVIAIIVIFAALLGFIQE
                     FRAEKAMSALKQLSAPTALALRDGEVQKIAARNVVPGDIIHLHAGDRIPADARILESF
                     SLRTEEASLTGESLPVEKETMALAEDAALADRVNMVYSGTSVSYGRGVVVVTNTGLQT
                     EFGKIARMLESVEHARTPLQINLDKVGNALAKAAGAIVVLITILGVMRGQPFLDMILF
                     GIALAVAVVPEALPAVVTVSLALGVQRMIKRNALVRRLPAVETLGSVSVIGSDKTGTL
                     TKDEMTVRKIHLPDQRFDVTGSGYEPSGDFVGNGTSVSPSPELILALKAASLASDASL
                     ENKDGSWKITGDPTEGAMIVAAAKAGLDKDSLDNEFPRIHEIPFTSETKRMTTLHSEG
                     TSKTAYSKGAPESILPSCTHYLSQKGILALTDREKSEYLKEAAFLAGEALRVIAVAMK
                     QNANEENAEEGMIFLGLLGMIDPPRTEVKTAIEKCRRAGIKPIMITGDHPITAAAIAR
                     ELGILRNGKVITGVELDSMSPEEFKRQVESIEVYARVSPAHKLMVVHAFQKNGHSIAM
                     TGDGVNDAPALKKADIGIAMGITGTDVTREAAAMTLTDDNFASIVAAVEEGRGVFGNI
                     KKYLMYLLSSNIGEIGLMAGTSMLGLPLPLSAVQILYVNLATDGLPALALAVDPPDED
                     AMNQPPRNASAGIFTRPVVVLMLAGGIWSTLVNLGLFAWAQSSGRSLSEAMTMSFASL
                     VLIQFFKAYNYRSDQASVWHKPFANKWLNMAIAWELLLLSLVIYHPFLQKAFGTYAIT
                     AVDLVVILSLALSIIPVLETVKWLVRRGWFAQKQAAGAKPATHER"
BASE COUNT         1149 a         1096 c         1114 g          901 t
ORIGIN      
        1 caggttgcgg tggagaagca atgggaccgg tgcaaatcgg aaacaaaact cttcaggtcc
       61 ccggcgatgt agcatgcgcg ggagaagaga cacgcattta tgatcagaga gagcttttga
      121 tgaaggacct gggcattaag ccagaggacc tcggcattca gggcaatgtc cgattcaaga
      181 atgaaaagca ataattcccg cgtcgtcggg agggagaatc gttatgaaga gcacacaaat
      241 cgggaaagac agacgcgaag gtcaattcgc acagctgctc gaagcaagcc tgaaaaacca
      301 gactaaagtc gaagctggaa gttacgacct ggtaactgta tctgacatca caaacaaaga
      361 tttcgtgttt gttcaatcac ccagagggac cgggctcatt ccgcgcgaag agcttctcga
      421 tcatcatggc aagatccgcg tttcgcgcgg tgagaagatt ccagcattct atcgctccac
      481 agaaaacgga gaaatgatgt tcacaacgca gccttcaggc cgcgcgaaaa tggacattgt
      541 ccgcaccgcg attgaaaaca aaatcccaat gacaggacgt atcctcaaga aaatcaaagg
      601 cggatttgaa gttgtcattg gagaagtaaa cgcgttctgc cccgcatctc acatggacgg
      661 aaatccagac gtaaagtcag aagttacctt tctgatcaca gaagcaaagg gggaccgagt
      721 catcgcatct aacagagcgt ttcgcgacat cgaaaagaag cgccaaaaag atgtgctcat
      781 gcagtcactc cagccagggg atgtagtgga aggaaccgtg gccaacctcg cagactttgg
      841 cgcgtttatt gacattggcg gaatggaagg actggttcca ctttcagaga tcgcccacca
      901 gagagtgact catccatccc aggttctcaa atcgggacag agcgttcgcg taaaaatcct
      961 gcaaatcgat tggaaagacg atcgcctcac tctctctctt cgcgcactcc tcgaaaaccc
     1021 atggcagggt cagcttccct ggaaagaagg agatattgta gaaggcaaag tggaaagcat
     1081 caaacctttt ggagtattcg taaagctaga aggaaacttc acaggactga ttccagcagg
     1141 agaatcagga gtggcacgtg gacagccttt tgaaaagttt ttccagaaag gacaaactct
     1201 tcgcgtcatg gtgcacacca tcgaccgcga acgcgaaaag atttcccttt ccgtgcgcgg
     1261 agtcgcggca catgaccagg aacaggaatt cgcagagtac ttaaaagttc agggcccgca
     1321 gaaagaggag ctctccagtt ttggtcagca gcttcgcgat gcaatgaaca agaagaaata
     1381 aagcacacct gcgcgtggcc ttcgcgcgcg cccttcgggg ccagccatca tgaacgagcc
     1441 actctcaaag ccccatgccc tgagcactga tcaaatcctg gagctcgtga cctcatcgcg
     1501 gaccggactc tccgttgcag aagccgatcg tcgcctggcc ctctggggcc caaacgaaat
     1561 ccccactcaa aaaagatcct cctggattcg catcctggcc caacagctga agaatgtgct
     1621 catcatcatt ctacttcttg ccacagttct ttctgccgta cttggccata gcactgaagc
     1681 agcagtcatc gcaatcattg taatctttgc cgcgcttctc ggatttattc aagagtttcg
     1741 ggcagaaaaa gcaatgtccg ctctcaaaca attgagtgct cccacagcac tcgcattgcg
     1801 tgacggagaa gtgcaaaaaa tcgccgcgcg caacgtagta cccggagaca tcattcacct
     1861 tcacgccggt gaccgaatcc ccgcagacgc acgaattcta gagagcttca gtcttcgcac
     1921 agaagaagca tcgctaaccg gagaatcact acccgtagaa aaagaaacga tggctttggc
     1981 ggaagacgca gcactcgcgg atcgggtaaa catggtatac tccggaacaa gcgtgtctta
     2041 tggacgaggt gttgttgttg tgacgaacac gggcctgcag actgaatttg gaaaaatcgc
     2101 ccgcatgctt gaatccgtgg agcacgcgcg cactcctctg caaatcaatc tggataaagt
     2161 gggcaatgcg ctggcaaagg cagcgggcgc tattgtagta ttgatcacga tcctgggagt
     2221 tatgcgaggc cagcccttcc tcgatatgat cctgtttggg attgcactgg cggtggctgt
     2281 ggttccagag gctttgcctg ctgtggttac agtgtctctg gctctgggcg tgcaaagaat
     2341 gatcaagcgc aacgcgctcg tgcgaaggtt gccggcagtc gaaaccctgg gtagcgtttc
     2401 cgtgattggc tccgacaaaa cgggaacgct cacaaaagat gaaatgaccg tcagaaagat
     2461 ccatctaccg gatcagcgat tcgatgtcac agggtccgga tatgaaccgt caggcgactt
     2521 tgtgggcaat ggaacaagtg tctctccgtc gccggaactc attttagctc tcaaagccgc
     2581 ttctcttgca tcggatgctt ccctggaaaa caaagacgga tcctggaaga tcactggaga
     2641 tcccacggaa ggggccatga tcgtggccgc cgcgaaggcg ggcctggaca aagattcact
     2701 ggacaacgag ttccctcgca ttcacgaaat cccgttcacg tctgaaacaa aaaggatgac
     2761 cacgctgcac agcgaaggca ccagcaaaac tgcctactca aaaggcgctc cagagtcgat
     2821 tctaccttcc tgcactcatt acctctcaca aaagggaatt ctggcactca cagacaggga
     2881 gaagagtgaa tatctcaaag aggcagcctt tctcgcaggc gaagcgctca gagtaatcgc
     2941 ggttgccatg aagcagaacg caaacgaaga aaatgcagaa gaaggaatga tttttctggg
     3001 gcttcttggg atgattgatc cgcccagaac agaagtaaaa acagcgattg agaaatgtcg
     3061 tcgcgcaggg atcaaaccaa ttatgatcac tggcgatcat ccaatcaccg cggccgccat
     3121 cgcacgcgaa ctgggcatac tgagaaatgg caaagtgatc acgggagtcg aactcgactc
     3181 gatgagcccc gaagaattca aaagacaggt tgagtcaatc gaagtgtatg ccagagtttc
     3241 gcctgcgcac aaactcatgg ttgtgcatgc atttcaaaaa aacggacatt caatcgcaat
     3301 gactggcgac ggcgtcaatg acgctccagc gcttaagaaa gcggatattg gaatcgccat
     3361 gggaatcacg ggcacagacg taacgcgcga agcggctgcg atgactctga ccgacgacaa
     3421 ctttgcctcc atcgtggcag cagtggaaga aggaagaggc gtattcggaa acatcaagaa
     3481 gtatctgatg tatcttcttt cctcgaacat aggagaaatc ggactcatgg ccggtacatc
     3541 catgcttgga ttgccccttc cactgtccgc agttcaaatc ctgtacgtga acctggcaac
     3601 agacggcctg ccggcacttg cacttgcagt ggatccaccg gacgaagatg ccatgaacca
     3661 acctccgaga aacgcttccg ctggaatctt cactcgacct gtagttgtct tgatgctcgc
     3721 aggtggaatc tggtccactc tcgtgaatct tggtctgttt gcgtgggcgc agagttcggg
     3781 cagaagtctt tcagaggcaa tgaccatgtc ctttgcttct ctggttctga tccagttctt
     3841 caaagcatat aattatcgtt cggatcaggc gagcgtgtgg cacaagccat tcgcgaacaa
     3901 atggctgaat atggcgatcg cctgggagct gctccttctg agcctggtga tttatcatcc
     3961 cttcttacaa aaagcatttg gaacgtatgc gattacggcc gtggacctgg tggtaattct
     4021 atcccttgcg ctttccatca taccggttct ggaaaccgtg aaatggctgg tgagacgggg
     4081 ctggtttgcc caaaagcagg cagccggcgc gaagccggcc acgcatgaac gttaagcgct
     4141 cttctttttc tttgctgctg ttttctttgc agaatcaata ggagtggcta caggcgtatt
     4201 cactggaaca gaacgattcg ctggcagctt ctgcaccgca acccccaggc tatcctggac
//
LOCUS       DAOQJL010000330         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12564_length_4256_cov_4.252321, whole genome shotgun sequence.
ACCESSION   DAOQJL010000330 DAOQJL010000000
VERSION     DAOQJL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12564_length_4256_cov_4.252321"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLG78_13485"
     CDS             <1..708
                     /locus_tag="PLG78_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24180.1"
                     /translation="VLIVLVLFFALSPLGAWYYFTAGNGSRMQWVFGSSCNSGSRPIT
                     MIYDSDIQSLGVGYTTAVATVMTQWNAPGGSLVCPAATCDPTNATYQRTMTAAAVAAF
                     LANPTPNQIWVVWDSDGSVLSANGVDPNGTILGVGQPINLSPSRPQDICSGILFLNGK
                     LISASASPTLVFQRVLLHELGHVLGFAHSIAGNNNGTVTAATGPNLPAMFPFLYTNSP
                     TTLSPDDTAGVRAVYGP"
     gene            752..1264
                     /locus_tag="PLG78_13490"
     CDS             752..1264
                     /locus_tag="PLG78_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24181.1"
                     /translation="MKFWEKEKEDNTKELILLLAAAYLANPCNFTASPVLTSAQPSGT
                     MTGVAGSIKGKLLTRAGTPAISALVIAEDNAAIGVNYWATHSSINRDGSFQISGIPGG
                     TTVKLSFESILSDYNGRIDGHIDCFKTPSTFTAGWVTGGTLTTTRASGQNFSIVNATT
                     LDAGTIYLVN"
     gene            1264..2082
                     /locus_tag="PLG78_13495"
     CDS             1264..2082
                     /locus_tag="PLG78_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNE24182.1"
                     /translation="MPELPDLVYVARGLHDFISGRIIEQVEVREPIVVRNLTGEPFGA
                     CVTGRGILNVRRHGPFLVFELTGHRMIVAHPMLAGAFSIEPKSAGLITFIFKEGKLSY
                     LDAKKMGKIYVVDPGQEAQIPRFTTQGVDITSPEFTEDVFLGLIRTSRRQVRAFLMEQ
                     EQISAIGNAYADEILFEARIHPKTFCHQLGPDEQKVLYDSIRTVIDRGIKEVQGANAG
                     LHVKHRDHMLVRNKKNEPCPRCGAKIRRESVLGYDTFFCPVCQPAKRKLFIDWK"
     gene            complement(2066..3202)
                     /gene="hisC"
                     /locus_tag="PLG78_13500"
     CDS             complement(2066..3202)
                     /gene="hisC"
                     /locus_tag="PLG78_13500"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE24183.1"
                     /translation="MKPSEFVHSHIRHLDGYVPGLQTEDPEVIKLNTNENPFPIPASV
                     RNALVQEIDGKLLQKYPNPVSQRLREALAAKYGRPPECFLVGNGSDEILSILFRAVLG
                     QDKAMIAPYPTYSLYPVIAALSHSSCIEIQVREDWTLDFEAMLQKIQKGGIALAAFAN
                     PNAPTGIAESEKDILDFTSRNPSLTLVDEAYADFGNVTVFKNAGTSTYPRLLVSGTAS
                     KALSLAGARIGWLCAEESLIAELEKIKDSYNVSRLAQAAALAAVLDKSEFEKRRDEIV
                     NNRNFLTEGLDRLGFETLPSSTNFIFTRPPADLRALSQRGETAAGAYYRLLLENKILV
                     RYWSSEKLKEWIRITIGTRADMEKLLSTTDSIVSLAKREPISSQ"
     gene            complement(3199..3999)
                     /locus_tag="PLG78_13505"
     CDS             complement(3199..3999)
                     /locus_tag="PLG78_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE24184.1"
                     /translation="MRTRLALALVFVSVVPIHALEWLPLEQAMTRARVESRPIFVDVM
                     APWCGFCRKMREQVFPTREFQEAASSFVLARVNADEDLSARKYNVEGLPTLLMLDHNG
                     YLIGRSDGFTEPERLVRLMKEALGRAGTQAKVEKDARERPGVNSSYRAGLYYASINDS
                     PRARSHFLNAWQAGKAKPDPQSFDALYNAAVSSMEMKDYSGAVQLWSQYVDLYPSRDR
                     DFAYARYFRGLALRSLGKKEEAKADMAYASNNLPPGEDKNAAIRMAGK"
     gene            complement(4056..>4256)
                     /locus_tag="PLG78_13510"
     CDS             complement(4056..>4256)
                     /locus_tag="PLG78_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE24185.1"
                     /translation="IARCLRMLELPVDEEPETETIKKQYRTLLKKYHPDVNPAGLERT
                     RDINSAYTALVLGLQTPSRRPS"
BASE COUNT          959 a         1076 c         1097 g         1124 t
ORIGIN      
        1 gtcttgatcg ttctggttct gtttttcgcc ttatctccgt taggtgcctg gtactatttc
       61 actgccggca acggtagcag gatgcaatgg gtgtttggaa gttcgtgcaa cagcggctca
      121 cgacccatca ccatgatcta tgattcggac atccagtccc tgggagttgg atacacaact
      181 gccgtggcca cagtcatgac tcagtggaat gctccaggtg ggagtctggt ctgtcccgca
      241 gcaacctgcg atcctacaaa cgcaacctac cagcgcacaa tgacagctgc tgcggtggcc
      301 gcgttccttg ccaatcccac tcccaatcag atctgggtcg tttgggactc agacggcagt
      361 gttctttctg cgaacggcgt cgatccaaat ggcaccatac tcggagtggg gcaaccgatc
      421 aacctcagcc cttccaggcc tcaggacatt tgctccggaa tcctgttctt gaacggcaag
      481 ctcatcagtg cttctgccag tcctactctt gtctttcaaa gagtgctcct tcacgagctg
      541 ggacacgttc ttggatttgc tcattccatt gcgggaaaca acaatggcac agtgactgcc
      601 gccaccggac ccaacttgcc tgccatgttt ccgttcttgt atacgaactc tcctacgact
      661 ttgagtccgg atgatacggc cggtgtgagg gcagtgtatg gtccgtagcc tgtgcgtggt
      721 cgccttatca ttgttatgcg catgcagcaa tgtgaaattc tgggaaaagg aaaaagagga
      781 caatacaaaa gaactcatcc ttcttctggc tgcggcttat ctggcaaatc cttgtaactt
      841 caccgcgtct cctgtgctta cgagtgcaca accttctgga accatgaccg gagtggcagg
      901 aagcatcaag ggaaaactgc ttaccagggc gggcacccct gccatcagcg cgctcgtcat
      961 cgcagaggac aatgcggcca ttggagtgaa ctactgggcc actcattcaa gcatcaatcg
     1021 agatggatca tttcagatca gcgggatccc tggcggaacc actgtgaagc tttccttcga
     1081 atcgattcta tctgattaca acgggcgcat agacggccac attgattgtt tcaaaacacc
     1141 ttctacgttc acggctggct gggtcacggg cggcactctc actactactc gagcatccgg
     1201 gcagaacttc tccatagtca acgcaacgac tctggatgcc ggaaccatct acctggtgaa
     1261 ctgatgccgg agttgccgga tctagtctac gtcgctcgcg gcctgcatga ttttatttct
     1321 ggccgcatca ttgaacaagt ggaagtgcga gaacccattg tggttcgcaa tttgacggga
     1381 gagccttttg gagcctgcgt gaccggcaga ggaattttga acgtcaggcg acatggacct
     1441 ttccttgtat ttgaacttac gggacacagg atgatcgtgg ctcatccaat gctcgccggt
     1501 gcctttagca ttgaaccaaa gtccgccggt ctcatcactt tcattttcaa agaagggaaa
     1561 ctatcctatc tcgatgcaaa gaagatggga aagatttatg tcgtggaccc gggtcaggaa
     1621 gcgcagattc caaggtttac cacacaggga gtggacatca cttcccctga gtttacggaa
     1681 gatgtttttc ttggactcat tcgcacaagc cgccgtcagg tgcgtgcctt tctcatggaa
     1741 caggaacaga tcagtgccat cggcaatgca tatgcggacg aaattctttt tgaagctaga
     1801 attcatccaa aaacgttttg ccatcagctt ggcccggatg agcaaaaagt tctatatgat
     1861 tctattcgaa cggtcatcga tcgtggaatc aaagaagtgc agggagccaa tgccggcctg
     1921 catgtaaagc accgagatca catgctcgtg cgaaataaaa agaatgaacc ttgtccgcgc
     1981 tgtggtgcaa agatcaggag agagagtgtt ctgggctacg atactttttt ctgccccgtc
     2041 tgtcagccag cgaagagaaa gctgttcatt gactggaaat aggttccctt tttgcaagag
     2101 agacaatcga gtccgttgta gataggagct tttccatgtc ggctctggtt ccgattgtga
     2161 ttcgtatcca ctctttgagc ttttcggatg accagtatcg taccaaaatt ttattttcca
     2221 gaaggagtct gtagtacgca ccggcagctg tttcccctct ttgcgagagt gctcttagat
     2281 ctgcaggtgg tcttgtgaaa atgaagttgg tgctcgaggg cagtgtttca aaacccagcc
     2341 tgtccagtcc ctctgtgaga aagtttcgat tatttactat ttcatctctt cttttttcaa
     2401 attcggattt gtccagtact gcggccagcg cggctgcctg ggccagcctg gaaacgttgt
     2461 aggaatcttt gattttctca agctccgcaa tcaaggattc ttccgcgcac aaccagccga
     2521 ttcgtgctcc ggcaagagag agagctttgg acgcagtgcc gctgaccaga agtcgcgggt
     2581 aggtagatgt tcccgcattc ttaaaaacag ttacatttcc aaagtcagcg tatgcttcat
     2641 ctaccagggt gagagatggg ttgcgcgagg tgaagtccag gatatccttt tctgattccg
     2701 cgattccagt gggtgcattt ggatttgcaa atgcagccag ggcgattcct cctttctgga
     2761 ttttttgaag catggcttca aaatcaagag tccagtcttc gcgcacctgg atttcaatgc
     2821 acgagctgtg cgaaagtgcg gcgatcaccg gatagaggct gtacgtagga tagggcgcaa
     2881 tcatggcttt atcctgtcca agaactgcac gaaatagaat gcttaagatt tcatccgatc
     2941 catttccaac caggaagcat tccggcggcc ttccgtattt tgcagcgagt gcttctctta
     3001 atctttgcga aacagggttt ggatacttct gtagaagctt gccgtcaatt tcctgaacca
     3061 gagcgtttcg cacggatgcg ggaatgggga acggattttc attcgtattg agtttgatga
     3121 cttccgggtc ctctgtttga aggccaggaa catatccgtc taaatggcgg atgtgtgagt
     3181 gaacaaattc agatggtttc acttcccggc cattcggatt gccgcgttct tatcttctcc
     3241 aggcggcaga ttgtttgatg cgtatgccat gtccgctttt gcctcttctt ttttcccaag
     3301 gcttcgtaga gcgagtcctc taaaatagcg cgcataggca aagtccctat ctctggatgg
     3361 gtagagatct acatactgtg accagagctg caccgcaccc gaatagtctt tcatttccat
     3421 agaactgact gctgcgttgt aaagtgcgtc aaaggattgc ggatctggtt ttgctttgcc
     3481 tgcctgccac gcattcagga aatgagagcg cgctcttgga gaatcattga tgctcgcgta
     3541 gtagagtccc gcgcgataag aggaattcac tcctggcctt tcgcgtgcat ctttttctac
     3601 ttttgcctgt gtgcccgccc gaccgagtgc ttctttcatc aatcgcacca gtctttccgg
     3661 ttcagtgaat ccatctgatc ttccaatcag atatccattg tggtcgagca tgagcagagt
     3721 gggcaggccc tccacgttgt atttgcgcgc agacaaatct tcatccgcat tcacgcgggc
     3781 cagtacaaaa gacgatgctg cctcctggaa ttctctggtt gggaaaacct gttcgcgcat
     3841 tttgcggcaa aagccgcacc agggcgccat cacatccaca aagatgggtc tggattcaac
     3901 gcgtgcgcga gtcatggcct gctccagagg aagccattcg agtgcgtgta tgggcactac
     3961 actcacgaat accagagcca gggccagtcg ggtccgcatg actacattta cgcgaatcgg
     4021 ggtggatctg tcaacgtttc gttgccaggg ggcattcagg acggtctgcg gcttggagtc
     4081 tgcagcccta gaaccagggc cgtataggcg gaattgatgt ctctggttct ttccaggccc
     4141 gccggattca cgtccggatg atattttttg agcaaggtgc gatattgttt ctttatggtt
     4201 tctgtttccg gctcttcgtc gacgggaagt tcgagcatgc gaagacaacg ggcaat
//
LOCUS       DAOQJL010000331         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12609_length_4245_cov_4.224344, whole genome shotgun sequence.
ACCESSION   DAOQJL010000331 DAOQJL010000000
VERSION     DAOQJL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12609_length_4245_cov_4.224344"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1447)
                     /gene="creC"
                     /locus_tag="PLG78_13515"
                     /pseudo
     CDS             complement(71..1447)
                     /gene="creC"
                     /locus_tag="PLG78_13515"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
     gene            complement(1485..2144)
                     /gene="creB"
                     /locus_tag="PLG78_13520"
     CDS             complement(1485..2144)
                     /gene="creB"
                     /locus_tag="PLG78_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HNE24186.1"
                     /translation="MKILIVEDEPAIADALLFALRADGFTADHARTAQEARPLVADAD
                     LLILDVGLPDTNGFDLLRDLRTKSQVPVLILTARSEEVDRIVGLELGADDYVTKPFSP
                     REVVARARAILRRSSKKPDKQSAFDVDESRAVIRYFGKVLILSRTEYRMLALFLSRPG
                     WVFTRENIMDAAWEEPEESFDRSVDTHIKNIRAKLKEIRSDLDPIVTRRGIGYSIQED
                     L"
     gene            complement(2141..3697)
                     /locus_tag="PLG78_13525"
     CDS             complement(2141..3697)
                     /locus_tag="PLG78_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNE24187.1"
                     /translation="MNAETRIERVTIYSDRARIERVARLHIPPGRHVIAIPDLPVLAD
                     ENSVRASVRGAKLLSLDVKRSRYLEPPSANAHELEKKIEDLTDQISLIDRSIAVEQER
                     SAHFEKLGGETLAIARGIASGRMEISKHTELESSIAEEKKASTNRIIQLSKQKRQTVR
                     ELAKADLEYKNAGNAVSRERIECSLEVESEGKEVEMQISYIVGSASWLPRYDFRLGSK
                     LLMQYLGAIRQKTGEAWQNVELSLSTAPASDQIAPVEFSTWFLHEYVPPPPRPAAAPG
                     MIGAPPMQASARAADAPFEQEEVDALLYAGMEDSTSVSSGASITYTLTSRFDIPGNGE
                     EKKVPVTVQEFARELEYVTAPANGEQAFRRVRFTNDSAYLLLGGPGFVYYDQDFVGNT
                     SIEETPPGQKRELYLGIESRIRVKRELKLRETDKKLLGDTKRIRFRYQIDLENHLPEE
                     AVIILREQIPASTHASIKVKLETQESKPELPLPGILEWKFTLPAGAKREVSYDYVVEH
                     PQNMTILGLH"
     gene            complement(3739..>4245)
                     /locus_tag="PLG78_13530"
     CDS             complement(3739..>4245)
                     /locus_tag="PLG78_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNE24188.1"
                     /translation="TTSNRSEGSVGYCTMDGDTSGGLAPIAGVDKAYLKQWLLWMEKT
                     GDEFGPVQALRYINEQEPTAELRAGQTDERDLMPYSLLDQIEALAVRDKKSPKEIYQI
                     LSGKSIPDLSRHIARFFRLWTQNQWKRERLAPSFHLDDRNIDPRSWMRFPILSAGYAE
                     EIAELDSI"
BASE COUNT          896 a         1077 c         1071 g         1201 t
ORIGIN      
        1 attcgccgcg aaagtaagca ggattctgaa ctgggcagag ctggagtgat tttcaccagg
       61 ttttagcata tcatagcgga atagaaatag aagcgcacgc gccactccct gtgttctgga
      121 tctctgcttt tcctccgtgc agttccgccg cctcctttac aaagcacaaa ccaagtcctg
      181 tgctctttcg tttgttgtca ggacgtggca gggaataaaa tctttcaaac gctcgcgaga
      241 tagcgaattc aggaatgccc gggccttgat ctgttacgga aaattttaga aagccttctt
      301 ccattttcgt ttccagtgca atggtagaat ggtccggtcc aaagtcactt gcgttttcta
      361 ctagattgac cagggccgat tccagtacag cgcgcgatcc tgcaatcgtc acatcttctg
      421 atttaacttc cagccgctgc gaacgcttct cgagcctgga tgcagtgcga aggcgaattt
      481 cctggatgag ttcgtttgag ccaatccgtt cgcgcgatgc gagagacttc tgaccttcta
      541 tgcttgcgag ttcgagcagt cttctggtga ggtcttctat gcgagacgat tcggtgagta
      601 tgttttcaag aaattttttc ctggattcct catccgggtt tccttctgct agaatttctg
      661 cggcggcgcg aatggatgtg acagggctct tgatttcgtg ggtcattgtt tttacgtact
      721 gctccacgta agattttcct tccagttcct gtctcaggtt ttcaaattca cgcgccagct
      781 ctccgatttc gcctgctcca acatccggtg gtgagagcct gccgtgcttt cgcacagaaa
      841 ctatgtactg tgtgaggatt cgaagcggcc tcgtgaccca gaagaaagtg gccagagtca
      901 agagagtcat ggcgatcact gcaaagccgg ccccctggag taattttctc tccgcgattc
      961 gcacaaatgg ctgaatggaa tcgtttggtt tgaccactgt caccacgcct acaatttgcc
     1021 catcgtgccg aacgggcgcg gcaacgtaga gagatccggt catcggatca tccttcttga
     1081 tgcgcgtggc gcgcactccg tattctcctc tcaatgttcg cctgacgtca ttccagcttg
     1141 agaaatcctg acctgtggat tttccttcgg agtcgtagat tacaattccg cgaccgtctg
     1201 ttacatatac gctcacgttg attttgtctt tcttcaaagc atagatcttt gccttgaaag
     1261 ccatcgatcc tgcccgcgaa actacttctt tgagaagagc agggtccggt gcggcggaag
     1321 cgtccctttc caggagagct gccagaagat gcgctgtgtc gttgagtgat tcttccaggg
     1381 cttccaggta gcggggtctg atttcatcct ggatcaggcg atacagtaga aagaatccgc
     1441 ctgcgaaggt aaaaaaaaac tgaggaagat tttagcggtg agtttcacag atcctcctgg
     1501 atcgaataac caatgcctct tctcgtaaca atcggatcca ggtccgaacg gatttctttg
     1561 agtttcgcgc ggatattttt gatgtgggtg tccacggatc gatcaaagct ttcttctggc
     1621 tcttcccagg ccgcgtccat gatattctct cgcgtgaata cccagccagg tctggaaaga
     1681 aaaagagcga gcattctgta ttccgtgcgt gatagaatga gcaccttgcc gaagtagcga
     1741 atgactgctc tgctttcgtc cacgtcaaaa gcgctttgtt tgtccggctt ctttgaactg
     1801 cgtctcaaga tggcgcgtgc tctggccacc acttcgcgcg gggaaaatgg tttggtcaca
     1861 taatcgtctg cacccagctc caggcctaca atgcgatcca cttcttccga tcgcgcggtg
     1921 aggatcaaaa cgggtacctg ggatttcgtg cgaagatcgc gcaatagatc aaagccgttt
     1981 gtatcaggaa ggcctacatc caggatgagc aagtctgcgt ctgcgaccag aggtcgcgct
     2041 tcttgtgctg tccttgcatg atctgcagtg aagccgtccg ctcgaagagc aaacaacaga
     2101 gcatccgcaa tggcgggttc atcttccacg atcagaattt tcaatgcagc cccagaatgg
     2161 tcatgttttg aggatgttcc accacgtagt cgtaggatac ttcgcgcttt gctccagcgg
     2221 gcaaagtgaa cttccattcc agaatgccag gtagaggaag ttctggtttt gattcctggg
     2281 tttccagttt gaccttgatg ctggcgtgcg tggacgctgg aatctgttcg cgcaggatga
     2341 tgactgcttc ctcgggcaga tgattttcta aatcgatctg gtagcggaag cgaattcttt
     2401 ttgtgtctcc caggagtttc ttgtctgttt cgcgaagctt gagctcgcgt ttgactcgaa
     2461 tgcgactctc aattcccagg tatagttctc tcttttgtcc cggaggcgtt tcctctatgg
     2521 aagtatttcc cacaaaatcc tggtcgtagt acacgaatcc tggtccacct aacagcaagt
     2581 aggcggaatc atttgtgaaa cgcactcttc tgaatgcttg ctctccattt gccggtgctg
     2641 tcacgtattc tagttcgcgc gcaaactcct gaaccgtaac gggcactttc ttttcttccc
     2701 cgtttcccgg aatgtcaaat ctggacgtga gagtgtatgt gatgcttgcg ccagaggaca
     2761 cagaagtaga gtcctccatg cccgcgtaga ggagggcgtc tacctcctcc tgctcgaatg
     2821 gagcatctgc tgcgcgcgcg cttgcctgca tgggtggagc tccaatcatt ccaggtgcgg
     2881 cggcgggacg aggaggcgga gggacgtatt cgtgtaaaaa ccaggtggag aattccacag
     2941 gagcaatttg gtctgaggcg ggagccgtgg acaaagagag ctccacattc tgccaggctt
     3001 caccggtttt ctggcgaatg gcacccaggt attgcatgag tagtttcgat ccaagtctga
     3061 aatcatacct cggaagccag gacgcagatc ccactatgta agagatttgc atttctactt
     3121 ctttgccttc tgattctact tccagggagc attcaattct ttctctcgaa accgcattcc
     3181 ccgcgttctt gtattctaaa tctgcttttg ccagttctcg cactgtttgt cttttttgtt
     3241 tagaaagttg aatgattctg tttgtgcttg cttttttctc ttctgcaata gaagattcaa
     3301 gctccgtatg ttttgaaatt tccattcttc cggaagcgat tccgcgcgcg atggccagcg
     3361 tttcccctcc gagcttttca aaatgcgcgg atctttcctg ttccacagcg atgcttcgat
     3421 cgatcaaaga tatttgatct gttagatctt ctattttctt ttcaagttca tgcgcatttg
     3481 cagaaggagg ttccagatag cggctgcgct taacatccag ggaaagaagc tttgctcctc
     3541 gcacagaggc tcgaacgcta ttttcatccg ctagaacggg caaatccggg atggcgatga
     3601 cgtgtctgcc cggtggtatg tgaagcctgg cgactctctc aatgcgcgct cgatcgctat
     3661 agatggtgac gcgttctatt ctggtttctg cgttcattcc tttgcttgag aaatgaaccc
     3721 ccgatccatt cggtcaagct atatggaatc cagttccgcg atttcttctg cataccccgc
     3781 ggatagaata ggaaatcgca tccagcttcg cggatctatg tttctgtcat ccagatgaaa
     3841 ggaaggagcg agtctctctc tcttccattg gttttgagtc cacagtctaa agaatcgggc
     3901 aatgtgcctg gaaagatccg ggatggattt cccggaaaga atctgataga tttcctttgg
     3961 cgattttttg tctctcactg cgagagcttc gatctgatcg agcagagaat agggcatgag
     4021 atctctttca tcggtctggc ccgctctcag ttctgcggtt ggttcctgct cgttgatgta
     4081 gcgaagagcc tgtactggcc cgaattcgtc tcccgtcttc tccatccaca gaagccattg
     4141 ctttaagtat gctttgtcca ctccggcgat gggagcaagg ccgcctgacg tatctccgtc
     4201 catggtgcag tagcccacgc ttccttcgct gcgattgctt gttgt
//
LOCUS       DAOQJL010000332         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12610_length_4245_cov_4.166587, whole genome shotgun sequence.
ACCESSION   DAOQJL010000332 DAOQJL010000000
VERSION     DAOQJL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_4245_cov_4.166587"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLG78_13535"
     CDS             <1..955
                     /locus_tag="PLG78_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE24189.1"
                     /translation="GVAIVVAAGNDGSNLNSANTYPCEYSQDNVLCVAALDQSYSLAT
                     FSNYDTTSVALNNVDVGAPGTNILSSFAGVTTTVNDTFNTSGTLNWSTTGSGWGYSAC
                     NLGQVVDVIGNPTTWCSLGQYANNANDTIYKTFNVPSGADMVSVAYGLFLDVASGDTF
                     SVHYKSGSGEPFSSGTQLASYTNVSTGGSPVFLGHELKNCAGQSQCSVGYRLVTNASG
                     TTYGPGILLFSLIGMDVDATTQYKVEDGTSMASPAAAGVVALVLSYNPSYTYLDALNA
                     VLNSGNAQTALNGKSKTGKSVDANNAIRYVAPPTGISLTAQ"
     gene            952..1263
                     /locus_tag="PLG78_13540"
     CDS             952..1263
                     /locus_tag="PLG78_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24190.1"
                     /translation="MRQFLCWAFMSLSIQAAQMGQVIPGEYLVRSEKSIEQLERAFSS
                     TGSVSVSRVEPGLYLLRFEKDPGLDVLVRLSAKEGLGTVQPNFRYSTQPGSGGLHRPV
                     R"
     gene            complement(1205..1891)
                     /locus_tag="PLG78_13545"
     CDS             complement(1205..1891)
                     /locus_tag="PLG78_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE24191.1"
                     /translation="MFGWLFLLLPVYNYFGASQRLHVPMNYPEAILLRMSVIEVILLF
                     AAWPVACGFLLGKKWGWWSFLTYGAALIAHNLTAVLYWRAIGGVSALVQTMVLIAIIL
                     YVTRPDISAPFFRTYPRGWRLQKRRPVKTSVLVEGKELQTRDISPRGIYITEALANQS
                     PGAPVNVQFVVHGPVVNGAIVRIDDAGTGIAFRGLNSSQKKLISEWVKQVTEQGDASP
                     RNQAAWNSES"
     gene            complement(2023..3858)
                     /locus_tag="PLG78_13550"
     CDS             complement(2023..3858)
                     /locus_tag="PLG78_13550"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24192.1"
                     /translation="MRTLAFLIPLTLCIACAHTIEPSGPVILLSPGALDGDIPLSISG
                     PVEFYWSRLIAPEEFRLKDRPQPDSILQQMGPWKAIAPASGRATYRFHLRCEPMPHKL
                     GLWFPHQFTASRVFANGKLIAETGIIGKTLGEDVIDRSDTLTSFDVSGPELELIFHIS
                     NQDSYRGGMRGSLVIGKPEVVSRYAQQRMLIEEIVLGIILGVAVYHLFFYFMHRTEYS
                     FLFFSILCFFLAARMPFQGSKIYHILLGPISWESQLRFLAVITIGAPPIGAALLRGLF
                     PDLVRLRSVFAYSAVAAISLLSQAGDLNVVSIATMLYAIVVYSFSGAHILFTTYRATR
                     AGAAAIPMALGVGSLSMLGILAFVQNFRGRDGGYYALAAFLFFVIFQALALSRFFLGA
                     VRARAEIAVSLQESRQALSQQRDDLQTSLHDSLGGALTDLQIHAEREMLSASGPSHDV
                     LAGIYNRIVDAVKMFRSQLLFMEDLSMTANDLLPGVQMTLLRRYADASREIDFDFTDE
                     AATCLSSQTEISFPLKRRLDLFFLIVELCTNDLKHGKGESLWRISTHEGKLCIVQKNG
                     KRTPEEISAESPASATDRVHRLRGTIKALSGVDEYSIRIEIPLNG"
     gene            complement(3867..>4245)
                     /locus_tag="PLG78_13555"
     CDS             complement(3867..>4245)
                     /locus_tag="PLG78_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE24193.1"
                     /translation="TNMNSDVMIFDSIKNGALGYILKSEIGNLEETVDVIGRGGATIT
                     PTIALRVFSTFRRVAEPGPDLTDREKQVLGLMVKGKSIHSVATFLGLSDHTVHGYVKS
                     IYKKLNVHNRAELAARAQELSLT"
BASE COUNT         1073 a         1055 c         1105 g         1012 t
ORIGIN      
        1 aggagttgct atcgtcgtcg ctgcaggaaa cgacggaagc aatctgaatt cagccaacac
       61 ctacccgtgc gaatacagtc aggacaacgt cctttgtgtc gcagccctgg accagagcta
      121 ctcgctggct acgttttcca attacgacac cactagcgtt gccttgaaca acgtggatgt
      181 aggtgctccg ggcaccaata ttttgagttc tttcgcagga gtaacaacca ctgtgaatga
      241 tacattcaac acatctggaa cactcaactg gtctacgact ggttctggat ggggttacag
      301 tgcgtgcaat cttggccagg tcgtagatgt gatcggcaat ccaacgacct ggtgttcgct
      361 ggggcaatac gcaaacaacg caaacgacac aatctacaaa acattcaatg tgccttctgg
      421 cgcagatatg gtatccgtcg catacgggtt gtttctcgat gtggcgagcg gggatacatt
      481 ctctgttcat tacaaaagcg gatccggcga accattctcc tccggcacac agctggcctc
      541 ctatacaaac gtttcaaccg gcggctctcc cgtttttcta ggtcatgaat tgaagaactg
      601 tgcagggcag agccagtgca gcgttggtta caggctggtg acaaatgcat cagggacaac
      661 ctatggcccc gggattcttc ttttttctct catcggcatg gacgtggatg cgacgactca
      721 gtacaaagtg gaagacggca cttccatggc ttcccccgcc gcagccggtg ttgtggccct
      781 ggttctcagc tacaatccgt cttacacata cctggacgct ctcaatgcag ttctcaacag
      841 cggaaacgcg cagacagctc tcaatggcaa aagtaagaca ggcaaatctg tagatgcgaa
      901 caatgcaatt cgctacgttg ctccaccgac tggaatcagc ctgactgctc aatgagacaa
      961 tttctctgct gggcattcat gtctttgtct atacaggcag cccagatggg gcaggtgatt
     1021 ccaggtgaat atctggttcg atctgaaaag tcaattgagc aactggagcg cgcgtttagt
     1081 tccactggat ccgtatccgt ttcgcgcgtg gaaccaggtc tctatctatt gcgatttgaa
     1141 aaagatccgg ggctggatgt tctggtgcgt ctttctgcga aagaaggact gggaacagtt
     1201 caacctaact ttcgctattc cacgcagcct ggttccgggg gcttgcatcg ccctgttcgg
     1261 taacctgctt cacccattcc gatataagtt ttttttgcga tgagttgagt cctcgaaatg
     1321 cgattccggt tccagcgtca tcgattcgaa caatggcacc attcacaaca ggaccatgga
     1381 ccacaaactg aacgttgacc ggggcacccg gcgactgatt tgcaagcgct tctgtgatgt
     1441 agatgccacg aggggaaata tcccgtgttt gcaattcctt accttccaca agcactgaag
     1501 ttttcacagg tcgccttttt tgcagacgcc agccgcgagg atacgtgcga aagaaaggag
     1561 cagaaatatc cggcctggtt acgtacagta tgattgcaat gaggaccatg gtttgcacga
     1621 gggcagacac tcctccgata gcgcgccagt agagaacggc ggtcaggttg tgcgcgatca
     1681 aggctgcccc ataggtgagg aaactccacc atccccactt ttttcccaga aggaaaccac
     1741 aggccacggg ccaggcggcg aacagtagaa tgacttcgat tacagacatg cggagcagga
     1801 ttgcttctgg ataattcatc ggaacgtgga gtctctgaga ggccccgaag tagttataga
     1861 ccggcagcag taagaacagc catccgaaca cgagcaatga tacgggtatt ttttttctgc
     1921 gtcttcgatc tagaaaaaac atgatgtatg gtgtcttcca atgtcccggt gtaaatcaga
     1981 atatggatga atttgaaaat gcttttgatt ttttcaacgg acttagccgt tcagcgggat
     2041 ctcaattcgt atgctgtatt cgtccacacc gcttaaggct ttgatcgtac ctcgcaggcg
     2101 atgcactcga tctgttgcgc tggcggggct ctcggcactt atttcttctg gtgttctttt
     2161 tccattcttc tgaacaatgc acagcttccc ttcatgggtg ctgattctcc agagagattc
     2221 tcccttcccg tgttttagat cattcgtaca cagttcaacg atcagaaaaa acagatccag
     2281 tcttctcttc aatggaaaag aaatctccgt ctgtgaagat agacaggtgg ctgcttcatc
     2341 cgtgaagtca aagtcgattt cgcgagaggc gtccgcataa cggcgtagta aggtcatttg
     2401 cacgcctgga agaagatcgt ttgcagtcat ggacaggtct tccatgaaca aaagctggga
     2461 acgaaacatt ttcaccgcgt ctacaattcg gttgtagatt cccgccagca cgtcatggga
     2521 tggacccgag gcagaaagca tttctcgttc tgcatggatt tgcaaatcag tcagtgctcc
     2581 tccgagacta tcatgcagag aggtttgcag atcatcgcgt tgctgcgaaa gcgcctgacg
     2641 gctttcctgg aggctcactg cgatttcagc acgcgcacgc actgcgccca ggaagaaacg
     2701 gctgagggcc agagcctgga aaattacaaa gaacaagaac gcggccaggg cgtagtagcc
     2761 gccgtcgcgt cctctgaaat tttgcacgaa cgcaaggatg cctaacatcg agaggctgcc
     2821 tacgccaagt gccatgggga tggcagcagc gccggcccga gtggccctgt acgttgtgaa
     2881 gagaatgtgc gcacctgaga aggagtagac gacaatcgca tacagcatgg ttgcgatgga
     2941 caccacattc aaatcacctg cctgcgatag aagcgaaatg gcagccacag cggaatatgc
     3001 gaagactgaa cgcagacgaa ccagatcggg aaataatcct cgaaggaggg ctgcgccgat
     3061 gggtggtgcg cctatggtga tgacagcgag aaatcgtaac tgactttccc aggatatggg
     3121 tccaagtaga atgtgataga tctttgatcc ttgaaagggc attcgcgcag ctagaaagaa
     3181 gcagagaatg gaaaagaaca gaaatgaata ctccgtgcga tgcatgaaat agaagaacaa
     3241 atggtagacc gcaacgccca ggatgattcc cagtacaatc tcctcaatga gcatgcgttg
     3301 ttgcgcatag cgcgacacga cttctggttt tccaatcacc agggaacctc gcattcctcc
     3361 tctgtagctg tcctgattgc taatgtgaaa gatgagttcc agctcgggcc cactcacatc
     3421 aaaagaagta agagtatccg atcgatcgat cacgtcctca ccgagtgtct tgccaatgat
     3481 tccagtttcc gcgatgagtt ttccattcgc aaatactcga gacgcggtaa actgatgcgg
     3541 aaaccacaga ccgagtttgt gaggcattgg ttcgcagcgt agatgaaacc tgtaggtggc
     3601 acgtcctgat gcgggagcga ttgccttcca cgggcccatc tgttgaagga tggagtctgg
     3661 ttgaggacga tcctttaagc gaaactcttc tggtgcgatg agtctgctcc agtagaactc
     3721 cactggaccg gaaatcgaca atggaatgtc cccgtcgagt gctcctggag aaagcaggat
     3781 gacgggtccg gaaggttcga ttgtgtgcgc gcacgcaatg catagtgtga gcggaatgag
     3841 gaaagcaaga gtgcgcaatg aatgtctcag gttagagaga gttcctgcgc acgggcagcc
     3901 agttccgcgc gattgtgcac attgagtttt ttgtaaatgg acttaacgta gccgtgaact
     3961 gtatgatcgc tgagcccgag aaacgtagcc acagaatgaa tgctctttcc tttgaccatc
     4021 agtccaagca cctgcttctc tcgatctgtt aaatcgggac cgggttctgc tacacggcga
     4081 aacgtggaga acactcgaag tgcgatcgta ggagtgatcg tagctcctcc gcgaccaatg
     4141 acatccactg tttcctctag attgccaatt tctgatttta gaatgtagcc aagcgccccg
     4201 ttctttatgg aatcgaaaat catcacatct gaattcatat tggtt
//
LOCUS       DAOQJL010000333         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12628_length_4241_cov_4.624940, whole genome shotgun sequence.
ACCESSION   DAOQJL010000333 DAOQJL010000000
VERSION     DAOQJL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12628_length_4241_cov_4.624940"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PLG78_13560"
     CDS             complement(<1..1159)
                     /locus_tag="PLG78_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017859926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24194.1"
                     /translation="MRFFAAVVMGFSLATVGLRADPVLLRAVQIDAQPDEVLRKLAAA
                     LNQDLKTVLESKGLQVTTQGAAEAAKPALRGIKECKNAECARTIYDQAGYTQILVPIL
                     QRNKGSENLLCYLYRVDGDRITDENLLILFINLPRDERRAAIASVMVQFFPDATGPVA
                     EVKPPEPEKKPEEKKPEPEIARKPEETKDQTRRDQKELNPEQSPKSEREPERTGPSLI
                     RIASNPVAEVFIDGTRVGTTPFEMPADGTLKTVRLTAKDHDDLVVPLRSMKSMEIMLT
                     LRRIRDYAPPSRSDVSPLGALARSLVLPGWGQYASGNQFSSYLFGAGSALALSTALYS
                     AKDMRQSRTKIVEQQRNFDLYVYAYGSGSTNLFLTQAGFASFASRDAFFENN"
     gene            1238..2266
                     /locus_tag="PLG78_13565"
     CDS             1238..2266
                     /locus_tag="PLG78_13565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE24195.1"
                     /translation="MSRFVAFISFLSIGSCYLAHAARGQASLLLGRQNIDDVIHSPDT
                     DENTRKKLEHVLAVRSWAHDKLGLSTNSFKKFTKLDRRAVAWNVSASRELRFEPHTWW
                     FPIIGTVPYLGFFEESKANAEADKLRREGWEAIVTEVAGYSTLGWFEDPLVSPQLEYS
                     EWGLTRLVIHECTHATVWLPGSVDFNESFASFVELEGAMTYVRDKYGVESEEYKKRIL
                     QEEESSRVRFLFHKLAQSLNTIYESNATDEEKRNRKRETIAAFRTTLLSKIPEFKAVN
                     MRAVAERDLNNAHMLSFLRYESGEEYFRDQFKECNAQWPCFLDRMKSLKKEPSGWKTS
                     RKVPDSGQ"
     gene            complement(2325..>4241)
                     /gene="ppdK"
                     /locus_tag="PLG78_13570"
     CDS             complement(2325..>4241)
                     /gene="ppdK"
                     /locus_tag="PLG78_13570"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNE24196.1"
                     /translation="RNPSTGERKFYGEYLMNAQGEDVVAGIRTPYPIGRLGDDMPEVF
                     GQLDEVQKKLEAHYHDMQDLEFTIQEKKLYMLQTRSGKRTGVAALRMAVEMVDEGLID
                     RKTALKRVDPESIPSMLAPVFDPEEKKKAIKEKRYLTRGLNAGPGAASGIVVFDSWNA
                     QNRKIKGEKTILVRMETSPEDIAGMEAAEGILTARGGMTSHAAVVARGMNKPCIVGCS
                     SLHVEERSGFMAVATEDGRSIIVREGESISIDGSTGEVMLGHLPTRPSEIQQVLDGLV
                     PEETSMVAKYFKKFMSWTDEVRTLQIRTNADTPRDARIARTFGAQGIGLCRTEHMFFE
                     GDRIAAMQEMILSDTVSAREKALEKLLPYQREDFIGIFEAMEGLPVTIRLLDPPLHEF
                     IPHDEGHMGELAHKSGVSLEKIRVRVEALKEQNPMLGHRGCRLGVTYPEITKMQVRAI
                     LEAAVTVKKKGKSVYPEIMIPLVGHIKELRNQKELIEATAQLVMRESNTRVDYLIGTM
                     IEVPRAAIVADEIAQEAKFFSFGTNDLTQMAMGFSRDDADKFLRDYLRLGFYERNPFQ
                     SIDQEGVGALVKMAIERGKKTRPDIKLGVCGEHGGDPTSIGFFHNAGLHYVSCSPFRV
                     AVARAAAARAALGV"
BASE COUNT         1016 a         1114 c         1026 g         1085 t
ORIGIN      
        1 gattattctc gaaaaaagca tctcggctcg caaagcttgc gaatccggcc tgcgtcagga
       61 acaaattggt ggaaccggat ccgtaggcgt aaacgtataa gtcaaaattc cgctgttgtt
      121 ctacgatttt cgttctagat tgtcgcatgt cttttgcgct gtaaagggca gtggataagg
      181 caagagcgct tccggcgcca aacaaatacg aagaaaattg attgcccgac gcgtactggc
      241 cccagccagg aagcaccaga gagcgagcaa gagcgcctaa cggtgaaacg tccgacctgg
      301 aaggaggcgc gtaatccctg attcgccgca gcgttaacat aatttccatg gacttcatgg
      361 atcgcagagg aactaccagg tcgtcatgat cctttgcggt aagacgcacc gttttcaaag
      421 tgccgtctgc gggcatttca aagggagtag tccctactct ggtcccatca atgaatacct
      481 ctgcgactgg attgcttgca atgcgaatca aagatggtcc ggtgcgctct ggctctcttt
      541 ccgatttcgg cgattgctct ggattcagtt ctttctgatc tcgccgggtc tgatcttttg
      601 tttcttctgg tttgcgcgcg atctccggct cgggcttttt ttcctccggt ttcttctcgg
      661 gttccggtgg cttaacctcg gctaccggtc cggtggcatc gggaaaaaac tgaaccatca
      721 cggatgcaat cgcggcacga cgctcatctc gcggcaaatt tataaaaagt atcagtagat
      781 tctcatccgt tatgcgatca ccatccactc gatataaata acacaataag ttctcgctac
      841 ctttgtttcg ctgcaagatg gggacaagaa tttgcgtata tcctgcttga tcgtatatag
      901 tccgcgcaca ctcggcgttc ttgcattcct tgatgccgcg aagtgcaggt ttggctgcct
      961 cagcagctcc ctgggtggtt acttgaagac ctttgctttc aagaacagtc ttcaggtcct
     1021 gattcaaagc agctgcgagt ttgcgaagaa cttcatccgg ttgggcatca atctgcaccg
     1081 cacgaagaag cactggatca gcccgaaggc caacggtagc taaagaaaaa cccatgacga
     1141 ctgcggcaaa gaagcgcata acccacctac gacgaaacaa cacgctcggt caaactattt
     1201 gacgaaaaaa cgaccttttg cccgatagat gatgttcatg tcccgtttcg tagcgttcat
     1261 ttcatttttg agcatcggta gttgctatct cgcgcacgcc gcccggggtc aggcgtctct
     1321 tttgctcgga cgacagaaca tagacgacgt aatccattca cccgacacag acgaaaacac
     1381 gcgaaagaaa ctggagcatg tgcttgccgt gcgatcctgg gcgcatgaca aactcggact
     1441 cagcacaaat tcctttaaaa aatttactaa attggacaga cgcgctgtgg cgtggaacgt
     1501 ctcagcttcg cgcgaactaa ggtttgagcc gcatacatgg tggtttccca tcattggcac
     1561 cgttccgtat ctgggttttt ttgaagaatc aaaagcaaac gcggaagcag acaagctaag
     1621 gcgcgaagga tgggaagcca ttgtaacaga agttgccggg tattctacgc tcggatggtt
     1681 tgaggatccg ctggtctccc cgcagctgga atactccgaa tggggtctta cgcgtcttgt
     1741 aatccatgaa tgcacgcatg caactgtctg gttacctggc agcgtggatt tcaatgaatc
     1801 attcgcttct tttgttgaac tggaaggtgc catgacttac gttcgcgaca aatatggagt
     1861 ggagtccgaa gagtacaaaa agagaattct gcaagaggaa gaatcgtccc gcgtgcgatt
     1921 cttgtttcat aagctcgcgc aatcactaaa tactatctac gagtctaacg caacggatga
     1981 agaaaaaaga aatcggaaac gtgaaacgat agccgccttt cgcaccacgc ttctgtccaa
     2041 aatcccagag ttcaaggcag tcaatatgcg cgcagtcgcc gagcgagacc tgaacaacgc
     2101 tcacatgctg agctttcttc gatatgaatc cggagaagaa tactttcgcg accagttcaa
     2161 agaatgtaat gcacagtggc cgtgctttct ggatcgcatg aagagtttga aaaaagaacc
     2221 atcgggatgg aaaacctcta gaaaggttcc agactccggg caatgagtca cgcgaccgcg
     2281 aagtcgattt cttccatttt cttcgcggcc ggagagcctt tgacctaaac gccgagagct
     2341 gcgcgggcgg cggcggctct tgccaccgca acacggaacg gtgagcagct aacgtaatgc
     2401 agacccgcat tgtggaagaa accaatgctc gtaggatctc caccgtgctc gccgcaaaca
     2461 cccagtttga tgtccggcct ggtcttttta ccgcgttcga ttgccatttt taccagtgcg
     2521 cctacgcctt cctggtcgat agactggaac ggatttctct catagaaacc aaggcgaaga
     2581 tagtcgcgca ggaacttgtc tgcatcatca cgcgaaaatc ccatggccat ttgcgtcagg
     2641 tcattggtcc cgaagctgaa aaattttgct tcctgagcaa tttcatctgc aacgatagcc
     2701 gcacgcggaa cttcgatcat ggttccgatc aaatagtcta ctcgagtatt agactctctc
     2761 atcacaagtt gggctgtagc ttcgatgagt tccttttgat tgcgtagttc cttgatgtgt
     2821 cccacgagcg gaatcataat ctcgggatac acgctcttgc ctttcttctt cacagtgacg
     2881 gcagcttcca gaatcgcacg cacctgcatc ttagtgattt caggatacgt cacacccagg
     2941 cggcaaccgc gatgtccgag catcgggttt tgttctttca gggcctctac acgaacgcga
     3001 atcttctcga gtgacacacc acttttgtgc gcaagctctc ccatgtgtcc ttcgtcgtga
     3061 ggaatgaact catggagagg tggatcgagt agacgaatgg tcacaggaag accttccatt
     3121 gcctcaaata ttccgatgaa gtcttctctt tgatatggaa gtagtttctc cagagccttt
     3181 tcgcgagcac tcactgtgtc cgagagaatc atttcctgca tggcggcaat gcggtcgcct
     3241 tcgaaaaaca tgtgctccgt tctgcaaaga ccaatgccct gcgccccgaa cgtgcgagca
     3301 atgcgcgcat cccgaggagt atctgcgttt gtgcgaatct gcagcgtccg aacttcgtct
     3361 gtccaggaca taaacttctt aaagtatttt gcaaccatgg aagtttcttc cggcacaagg
     3421 ccatcaagaa cctgctggat ttcggaaggt cttgtgggca aatgcccgag catcacttct
     3481 cctgtgcttc catcaatgga gatgctctcc ccttcgcgca cgatgatgga tctgccatct
     3541 tctgttgcaa cggccataaa accagatctc tcttccacat gtagagaaga gcaacccacg
     3601 atacagggtt tattcatacc gcgagcgaca accgctgcgt gcgaagtcat cccaccgcga
     3661 gcagttagaa ttccttctgc cgcttccatg ccagcgatgt cttcggggct ggtttccatg
     3721 cgtacgagaa tggtcttttc acctttgatc tttctatttt gtgcattcca ggaatcaaac
     3781 actacaattc ctgatgcagc tcctggaccg gcgttcagcc cacgagtcag atatcgcttt
     3841 tccttgatcg cttttttctt ttcctctgga tcgaatacag gagcaagcat cgatggaatc
     3901 gattctggat ctactctctt cagtgccgtc ttgcgatcga ttagcccctc atccaccatt
     3961 tcaactgcca tgcgaagtgc agccacgcca gtgcgttttc cgctcctggt ctggagcatg
     4021 tagagtttct tctcctgaat cgtaaattca agatcctgca tgtcatggta gtgcgcttct
     4081 agtttctttt gaacttcatc tagctggcca aagacttctg gcatatcgtc tccgagccgc
     4141 ccgattggat aaggcgtgcg aatgccggct accacgtctt ctccctgagc attcattaag
     4201 tattcgccgt aaaacttcct ttcgcctgtg gaaggatttc g
//
LOCUS       DAOQJL010000334         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12663_length_4234_cov_4.152668, whole genome shotgun sequence.
ACCESSION   DAOQJL010000334 DAOQJL010000000
VERSION     DAOQJL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12663_length_4234_cov_4.152668"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLG78_13575"
     CDS             <1..601
                     /locus_tag="PLG78_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNE24197.1"
                     /translation="VNMAMTLDGKTSAPGGGWKPGKADRIRMDHIRAECDALLIGVRT
                     LILDNPNVMVKHDIEKSPLPVIVVRKTLPPGNLRIFEGPRPPLIIADESQKGYLQTKE
                     LDGLEWEWSRGTDARSIVKILEKRGLRTLLVEGGPTMNDLFFAENLVDRLHLTLVSRV
                     LGGKADTVDRMPVHRAFKLVKQESADEELFLEYERVSQS"
     gene            complement(604..840)
                     /locus_tag="PLG78_13580"
     CDS             complement(604..840)
                     /locus_tag="PLG78_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNE24198.1"
                     /translation="MQIPLSVPDIGDAEKIELASWKVKAGDQVTEGQELCELVTDKAA
                     FPLEAPHSGTIVSLSRSTGDLVKVGEVLAVLDRA"
     gene            944..1921
                     /gene="nadA"
                     /locus_tag="PLG78_13585"
     CDS             944..1921
                     /gene="nadA"
                     /locus_tag="PLG78_13585"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNE24199.1"
                     /translation="MQATQDLREIRAKLEATMMPMELDHAMPIIEEIHKLKKQKNAVI
                     LGHNYMTPDVYHGVSDYIGDSLGLARQAATTNADIILFNGVHFMAETAKICNPDKLVL
                     IADLKAGCSLAESITGADVRKLREQFPGAPVVTYVNCSAEVKAETDICCTSANALKVV
                     ESLPDETVIFLPDAYLAKNVQLKTKKRIVSWEKGKCMVHEQYTGKDILDYRKQFDDLL
                     VIAHPECDTDVTSVADFSGSTSQMEQFIRQNKQKNVLLVTECSMSDNLRSMFPDRTFL
                     STCHTCPHMKKITLEKVRDSLLYNQFAVEVPEDIRVRAKRSVDRMLEIG"
     gene            1921..2403
                     /gene="ispF"
                     /locus_tag="PLG78_13590"
     CDS             1921..2403
                     /gene="ispF"
                     /locus_tag="PLG78_13590"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNE24200.1"
                     /translation="MDVRVGSGLDFHRLIENKTRPLMIGGIEITSDLALEGHSDADVL
                     LHALADAILGACGFSDIGTYFSDKDPANKNLNSSIILKRSLDMMRQKGYRLSNCDITI
                     IGEKPRMSPHREQIVDRLAELCEIDRDRIALKATTTEKMGALGRSEGLGCSATVLVVK
                     "
     gene            complement(2442..3572)
                     /locus_tag="PLG78_13595"
     CDS             complement(2442..3572)
                     /locus_tag="PLG78_13595"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24201.1"
                     /translation="MHRLWRFAFVVLVSPGFILSCKAQDPCGTGDISCSLIGASPFLR
                     LDRTFAYVSVGTTATIYRFYPDSGVIGATGTVPSAVISARDPNNKFMIGVVQAVPPNN
                     VYTYMINSYTGNLVSGAPNGSANNGPAYVDFHPSGRYLYTSNYSSLDISAFSIDPSSG
                     ALTKLGDFATSCGCANLAQIKVSPDGKYIYTTSNGGSNFISGFSINQSTGVPTFVSTT
                     VAAAGMDAILVDPTSSYVYGVSNAGTISGYSINTSTGVLTALSGSPFATTAGSLRGAM
                     HPSGRFLYTVNVAGGQLAKHDIATNGALGTATTISFGTNLQYVAIDPSGKFGFVNDGI
                     ASSFYIFAINQNTGAANLIPGNPIATSGPPGLPIITRPNAWL"
     gene            3938..>4234
                     /locus_tag="PLG78_13600"
     CDS             3938..>4234
                     /locus_tag="PLG78_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24202.1"
                     /translation="MLLVLAGIIGLQFLIHRTLLAQNLVLLLQIRIYKRRYKRVSLKN
                     RDRFFLGLFTRLWKSAVQYCFVVQPGTLVRFHRTLFTLFWRWISRTHAPGHPGLS"
BASE COUNT         1100 a         1083 c         1118 g          933 t
ORIGIN      
        1 ggtcaacatg gccatgacgt tggacgggaa aacgtcagct ccaggaggtg gatggaaacc
       61 ggggaaggca gatcgcattc gcatggatca cattcgcgcg gaatgcgatg cacttttgat
      121 cggggtgcgc accctgatcc tggacaatcc aaatgtcatg gtcaagcatg atatagagaa
      181 aagtccgctt ccggtgatcg ttgtgcgaaa gacactgcca ccgggaaatc ttcgcatttt
      241 cgagggaccg agaccacccc tcatcatcgc agacgaaagc cagaagggct acctgcaaac
      301 aaaagaactc gatggtctgg aatgggaatg gtccagggga acggatgccc gttctatagt
      361 aaagattctg gagaaaagag ggcttcgcac tttgctcgtg gaaggcggtc ccaccatgaa
      421 cgatttgttc tttgcagaaa atctggtaga tcgtctccac ctaacactgg tttcgcgagt
      481 actgggaggg aaagcagata cagtggacag aatgccggtt caccgggctt ttaaactcgt
      541 gaaacaagaa agcgcagacg aggagctctt tctggaatat gagagggttt cgcagagcta
      601 gagtcaggcg cggtccagga cagcgagcac ttcccctact ttcaccagat cccctgtgct
      661 gcgcgataga gaaacgatgg tacctgaatg cggtgcctca agtggaaagg cggctttgtc
      721 tgtcacaagc tcgcagagct cctgaccttc cgtgacctgg tcccctgctt tgactttcca
      781 ggatgcaagt tcgatttttt cagcatctcc aatgtcagga acggaaagag gaatctgcat
      841 gggcgatgct ccgcccaggg cccatgattc tcaaggaaaa ttccaaaaaa gattgcggcg
      901 ggacaagtcg aaccatgtct aactactgag tagaggaaat catatgcagg cgacccagga
      961 cctacgcgag atccgcgcga aactagaagc taccatgatg cccatggagc ttgaccacgc
     1021 catgcccatc atcgaagaaa ttcacaaact caagaagcag aagaacgctg tgatcctggg
     1081 acacaactac atgacgccgg atgtgtatca cggcgtgtcg gattacatag gggattcgct
     1141 gggcctcgcc aggcaggcgg ccacgacgaa tgcagacatc atactgttca acggcgtaca
     1201 cttcatggca gagacggcca agatttgcaa tccggacaaa ctcgttctga tcgctgacct
     1261 gaaggcggga tgctctcttg cagaaagcat cactggcgca gatgtgcgaa agctcagaga
     1321 gcaatttcca ggcgcacctg tagttacgta tgtaaactgt tctgcggaag taaaggcgga
     1381 gacagacatt tgctgcacca gtgccaatgc cctgaaagtt gtagaaagcc ttccggatga
     1441 aactgtgatt tttttgccgg acgcctatct tgcaaagaat gtgcagctca aaacaaagaa
     1501 aagaatcgtt tcgtgggaaa aaggcaagtg catggtgcat gagcaataca cgggaaaaga
     1561 catcctggac tatcgcaaac agttcgatga cctactcgtc attgcgcatc ctgaatgcga
     1621 cacagatgtg acatcagtgg cggacttttc cggaagcaca tcgcagatgg agcagttcat
     1681 tcgtcagaac aaacagaaga atgttctact ggtgacggaa tgcagcatga gtgacaacct
     1741 tcgttctatg tttccggatc gcacgtttct ttctacgtgc cacacctgcc ctcacatgaa
     1801 gaagatcact cttgaaaagg tgcgcgattc cctgctctac aaccagttcg ctgtggaagt
     1861 tccggaagac atccgggtgc gcgcaaagcg ttctgtagat agaatgcttg agattggctg
     1921 atggacgttc gcgtgggatc gggtctggat ttccacagac tcattgagaa caaaactcgc
     1981 cccttgatga ttggcggcat tgaaatcaca agcgatctgg cactcgaagg ccattccgat
     2041 gcagacgtgc tgctgcatgc tctggcagat gcaattctcg gtgcgtgtgg attttcagac
     2101 atcggaacgt atttctcaga caaagaccct gcaaacaaaa acctgaactc ttctatcata
     2161 ttgaagagaa gtctggacat gatgcgacaa aagggctaca ggctctctaa ttgcgacatc
     2221 acgatcattg gagaaaaacc gcgcatgtct cctcaccgcg agcaaatcgt cgatcgcctg
     2281 gcagagctgt gcgaaatcga ccgtgatcga atcgcattga aagctactac gacagaaaag
     2341 atgggcgccc ttggtagaag cgaaggcctg ggctgcagcg cgactgtgct tgttgtaaaa
     2401 tagaacgcgc ggcccggaac accgttagtc gctcttctga gctacaacca ggcgttgggt
     2461 cgagtaatga tgggcagacc aggggggcca ctcgttgcaa tcgggttccc cggaatcaga
     2521 ttggctgcgc ccgtgttctg attgatcgca aaaatgtaga aactacttgc tatgccgtcg
     2581 tttacgaacc caaactttcc ggacgggtcg atcgcaacat actgcaggtt tgtgccaaac
     2641 gaaatcgtcg tcgcagttcc gagtgctccg ttcgtggcga tgtcatgctt tgccagttga
     2701 ccacccgcaa cgttgacagt gtataaaaac ctgccggacg gatgcatggc accgcgcaag
     2761 cttccggcag tagtcgcgaa gggagaacca gaaagtgcag tcagcacacc cgtgctggta
     2821 ttgatgctat agccactgat tgtgccggca ttagagaccc cgtacacata actggaagta
     2881 ggatcaacca ggatggcgtc cataccggcc gcggcgacag tggtggaaac gaaagtggga
     2941 acgccggtgg actgattgat ggaaaaacct gaaataaaat tggacccacc gttggacgta
     3001 gtgtagatat acttcccgtc cggagacact ttgatctgcg caaggttggc acatccgcat
     3061 gatgtagcaa aatcgcctaa ctttgtcaga gcgcctgagg atggatcaat cgaaaacgct
     3121 gagatatcga ggctcgaata attggatgta tataaatatc taccgctcgg atgaaaatcg
     3181 acgtacgctg gcccgttatt cgcactccca ttgggtgcgc cactgactag attgcctgtg
     3241 taactgttga tcatgtatgt gtacacgttg tttggtggga ctgcctgcac tacgccaatc
     3301 atgaacttgt tatttgggtc ccgcgccgaa atcactgcac tcggtacggt gccagtggcg
     3361 ccaatcacgc ctgaatcggg gtagaatctg taaatggttg cggttgtgcc cacgctcaca
     3421 tacgcaaacg ttcggtccag acgcaggaag ggagaggctc caatcaaact acaggaaatg
     3481 tctcccgtac cgcaaggatc ctgagccttg caggatagga taaaacccgg agaaaccagc
     3541 acaacaaagg caaatctcca gagccgatgc aaaatcgcat tacaatccat atgcgggctt
     3601 acgcaatgag cggcagacgc cgcaaaatgc gagcacaatt ctagcctgaa aacgctcctc
     3661 gtgcggcctc cgcgcccgca gcataagcgc caggacattt gagactttca tttttgcgtc
     3721 cgcggaccgc gctcctgcgg gctccgcgtt cgcttcggtc tcgcttcgcg agactgcttc
     3781 gcaccctcca gatcgctggc cggaagttgt tcaagagcga tcaatgccgt gtaacgtcag
     3841 gtaagaggaa ctaatccgcc gcgaggcgga tttgcatcag aagaaacggc gccgattcgt
     3901 gcctcaattc cggcactact gcatccgatc attcccaatg ctgctcgttc tggccggaat
     3961 tattggtctg caatttctca ttcatcgaac tcttctggct cagaatctgg ttttgcttct
     4021 ccaaattcgc atctacaaac ggagatacaa acgagtctcc ctcaagaaca gagacagatt
     4081 cttcctcgga ctctttacgc gactctggaa atccgccgtt cagtactgtt ttgttgtcca
     4141 accaggcacg ctcgttcgat ttcacagaac cctgtttact cttttctggc gctggatctc
     4201 cagaactcat gcacccggac accctggact ttca
//
LOCUS       DAOQJL010000335         4229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12688_length_4229_cov_4.980115, whole genome shotgun sequence.
ACCESSION   DAOQJL010000335 DAOQJL010000000
VERSION     DAOQJL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12688_length_4229_cov_4.980115"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLG78_13605"
     CDS             <1..797
                     /locus_tag="PLG78_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE24203.1"
                     /translation="PAGPGNSERRARQYPASKLLIPAAVAQTWHMLRKHVPLLLILIL
                     PLGFCRDARVQVGFKTATVRAKKMPMRIDPLPLASEVEILSAGDKVELLKRSDKKFKS
                     GNTEDYWYYSRSPSGLEGWVYGGGLSIALAGSGEDNEKKYTEKEITDNLVGKWWELNP
                     DGSTGYRKIYFWPGGKYKHGTGAEELKEGKYEIKLADRLIKLKDGSPAGDELEFRFLG
                     QEMRLSGEYEGKNILFRRGEINPEAKEVSDEKKDKDKSAAEPKKQN"
     gene            complement(794..1513)
                     /locus_tag="PLG78_13610"
     CDS             complement(794..1513)
                     /locus_tag="PLG78_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24204.1"
                     /translation="MVAYRFFPGCARDHNCAVHLLSVVCLCEDKAYPISAACQSSVCL
                     TDCTVSPIFPRQVCANRGAMVAYRFCPGYASDPGENKVFPNHDAVVAYRSFPGYASDH
                     NCAVRLFSVVCLCEDKVYRISAICPISVCPNEVVSLIFPRQVCVSRAGMVAYRSFRSF
                     APDRNCAVRLFSVVCLYDYKVYQISAVSRISAAYPISACPSEVVSLIFPQVRANLCAV
                     IGLRNRSLVCVKLDVVGDSRN"
     gene            1771..2523
                     /locus_tag="PLG78_13615"
     CDS             1771..2523
                     /locus_tag="PLG78_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE24205.1"
                     /translation="MDLFEGSLAGKPSAEKEVRINRYLASAGIGSRRDVEDLVLKGRV
                     EINGRKVSDLFERVSAGDLVRVDGKLIRPLDHKYYIFNKPVGVVCSSRRFGDEEKIIY
                     DYLPEGLRDLKYAGRLDQDSRGLVVLSNDGDFIQAITHPSHRITKKYLVTAEYLPPEN
                     VLRRTFVKGIEHDGDILKAISAHIRDRHQGLVEVVLGEGKKRQIRRMFESVGSNVVDL
                     FRTTVGFCDIEKEPLKEGELRAISPEQLLYGE"
     gene            2527..3969
                     /locus_tag="PLG78_13620"
     CDS             2527..3969
                     /locus_tag="PLG78_13620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE24206.1"
                     /translation="MLFVTAQFLVFLVCTVVIYWVLPPKFRIWFLTAASLFFYATWNP
                     RFMLHFLVVVGVNWSVIELWRVYRKNWIFYVLQLANLCNLAFFKYFYFFADIIGRSAG
                     ISWLEQPAFQDSQKALGYEILLPLAISFYTFQIMSYGFDIFRGVYTEKNSLLELLLFK
                     AFFPQLIAGPIMRSAELLPQIRQCRTEGLTLDQNSFYKGLWLCMIGVVKKVLIADRLM
                     EYLGPIIGTKDPSVYAPLYLWLAVIAFAAMVYSDFSAYSDLARGFGLLLGFQIPVNFK
                     APLFFISVTDFWRRWHITFSRWLRDYIYIALGGNRVSKARLYFNFIFTFFIGGLWHGA
                     AYTYVVWGILLGVMLSIESFMESKGIPEFPVTWWGRILRRLLGHLMFWPTTVFFMGTS
                     WTWCMHALSSMANLPRLVVADGLEPMHVETALYAMAGVLVFHLFDEYPERFAKLRRAE
                     RILVPAGGLALILILSQFTGGQKDFFYFQF"
     gene            3977..>4229
                     /locus_tag="PLG78_13625"
     CDS             3977..>4229
                     /locus_tag="PLG78_13625"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE24207.1"
                     /translation="MKISWKLLLAPVFALFVLSYVDKAFLLPEVRDRFLQPGGMVYYK
                     QRERQIDRMREYAKAKPPDIHLAVVLGDSRSFAIGNLPAQ"
BASE COUNT         1045 a         1057 c         1135 g          992 t
ORIGIN      
        1 aacctgctgg tccaggcaat tcagagcgcc gagcgagaca atatcctgcc agtaaattat
       61 tgattccggc ggcagtcgcg caaacctggc acatgctccg caagcatgtg ccactcctct
      121 tgattctcat actgccgctt ggtttttgcc gcgacgcaag agtgcaggtc ggctttaaga
      181 ccgccacagt gcgcgcgaaa aagatgccca tgcggatcga tccacttcct cttgcctccg
      241 aagtcgagat cctgagtgcg ggagacaaag tagaactgct caaaagatca gataagaaat
      301 tcaaatctgg aaacacggaa gattactggt actattcgcg ctctcccagc ggcctggaag
      361 gctgggttta tggcggaggt ctttcgattg ctcttgccgg ttccggggaa gacaacgaga
      421 agaagtacac agagaaagag atcacagaca atctcgttgg caaatggtgg gagctcaatc
      481 cagacggttc caccggttat cgcaaaatat atttctggcc aggtggcaaa tacaagcacg
      541 gaactggggc agaggagctc aaagaaggga aatacgagat taagctggcg gacaggctca
      601 tcaaactcaa agatggctca ccggccggcg atgagcttga attccgcttc cttggccagg
      661 aaatgaggct ttcgggtgaa tatgaaggaa aaaacatcct cttccggcgc ggagagatca
      721 accccgaagc gaaagaagtt agtgatgaaa aaaaggacaa ggacaagtcc gcagcagagc
      781 ccaaaaagca aaactagtta cgagaatcgc ccaccacgtc cagcttcacg cagaccagac
      841 tccggttacg aaggccgatc accgcgcaga gattcgcgcg gacctgcgga aagatcagag
      901 agaccacctc gctcggacag gcagagattg gataggcagc agagatccga gagaccgcag
      961 agatctgata gaccttatag tcgtacagac agaccacaga gaacagacgg accgcgcagt
     1021 tacgatcggg tgcgaagctc cgaaaggaac gataggcgac catcccggcc cgactcacgc
     1081 agacctggcg cggaaagatc agagagacca cctcgttcgg acagacagag atcggacaga
     1141 tcgcagagat ccgatagacc ttatcctcgc acagacagac cacagagaac agacggaccg
     1201 cgcagttatg atcgctcgcg tagcccggaa aggaacgata ggcgaccacc gcgtcgtgat
     1261 tcggaaagac cttattctcg cccggatcgc tcgcgtagcc cggacagaag cgataggcga
     1321 ccatcgcgcc ccgattcgcg cagacctggc gcggaaagat cggagagacc gtacaatcgg
     1381 tcagacagac agagctctga caggccgcag agatcggata ggccttatcc tcgcacagac
     1441 agaccacaga gagcagatgg accgcgcagt tatgatcgcg cgcgcagccc ggaaagaaac
     1501 gataggcgac catctcgccc tgattcgcgc agacctggcg cggaaagatc ggaaagaccg
     1561 tacagtcgct cagacagacc ggacagacaa agatcggaca ggccttatcc ccgctcggac
     1621 agaccgcaga gatccgacaa gccttatcgc gcagagccgc gccgctcgga agcattcagc
     1681 acgagcgacg aagatggtgc acagttttct tatgttccgc cggacccgtt tccaaagcaa
     1741 agaaaagtag cggccgcccc gtccgaagcg atggacctgt ttgaggggtc gctggcgggt
     1801 aaaccctcgg cggaaaagga agttcgaatc aaccgttatc tggcatccgc cggaattggg
     1861 agcagacgcg acgtagaaga tctggttctc aaaggcagag tagaaatcaa cggcaggaag
     1921 gtgagcgacc tttttgaacg cgtttccgcc ggtgatctgg ttcgagtaga cggtaaactg
     1981 atccggcctc tggatcataa gtattacata tttaataagc ccgtgggtgt ggtctgttcc
     2041 tcgaggagat tcggggatga agaaaagatc atctacgatt atcttcctga aggtttgcgc
     2101 gatttgaagt atgcgggtag actcgatcag gattctagag ggcttgtagt gctttccaac
     2161 gacggagatt ttatccaggc tatcacacat ccttcgcatc gcatcacgaa gaagtatcta
     2221 gtaaccgcag aatatcttcc gcctgaaaac gttttgcgca gaacgtttgt aaagggaatt
     2281 gagcacgatg gggacattct caaagctatt tctgcgcata tccgggatcg tcatcagggc
     2341 cttgtggaag tggttctggg ggaaggaaag aagaggcaaa tccgcagaat gttcgaatca
     2401 gtaggctcaa atgttgtgga cctatttcgc actaccgttg gattctgcga cattgagaaa
     2461 gaacctttga aagaaggtga acttcgcgcg atcagccccg agcagctttt atacggagaa
     2521 taatcgatgc tgtttgtaac cgctcagttc ctggttttcc ttgtctgtac ggttgtcatc
     2581 tactgggttt tacccccaaa gtttcgcatc tggtttctca ctgccgcttc tcttttcttc
     2641 tacgcaacct ggaacccacg tttcatgctg catttcctgg tagtggtcgg agtcaactgg
     2701 agtgtgatcg aactctggcg cgtttatcgc aagaactgga tcttctatgt tctacaactg
     2761 gcgaatctct gcaacctggc attctttaag tatttttact ttttcgctga tatcattgga
     2821 cgatcggccg ggatttcgtg gctcgagcag ccagccttcc aggattcgca aaaagcgctc
     2881 ggctacgaga tacttctccc cctggccatc agcttctaca catttcagat catgtcctat
     2941 ggatttgata tctttcgtgg agtatacacg gagaaaaata gtctgctgga acttcttcta
     3001 ttcaaagctt tcttcccgca gctcatcgct ggccccatca tgcgatctgc cgaacttctt
     3061 cctcagattc gccagtgcag aacagaaggt ctgaccctgg atcagaattc tttctacaaa
     3121 ggtctctggt tatgcatgat cggagttgtg aagaaagtac tcatcgcaga cagactcatg
     3181 gagtatctcg ggccgatcat aggcacgaaa gacccgtctg tctatgctcc tctgtatcta
     3241 tggcttgcgg tgattgcgtt tgcggccatg gtgtactctg atttttccgc atactctgac
     3301 cttgcgcgcg gattcgggct tctacttgga tttcagattc cggtgaattt caaggctccg
     3361 ttgtttttca tttctgtcac cgatttctgg cgcagatggc atataacgtt ctcgagatgg
     3421 ttgcgagact atatttacat cgcactgggt ggaaatcgtg tttcaaaagc aaggctgtac
     3481 ttcaatttca tcttcacttt cttcattggc ggactctggc acggtgcggc ttacacatac
     3541 gtagtgtggg gcatcttgct cggtgtgatg ttgtccattg aatcgtttat ggagtccaaa
     3601 gggattccag aatttcctgt gacgtggtgg gggaggatcc ttcgccgttt gctcggtcat
     3661 ttgatgttct ggcccacaac tgttttcttc atgggcactt cgtggacctg gtgtatgcac
     3721 gcgttgagtt ccatggcaaa cctcccacgc ctggtggtgg cggatggact ggagcccatg
     3781 catgtggaga cggctctcta tgcaatggcg ggtgtgcttg tctttcattt gtttgacgag
     3841 tatcctgagc gtttcgcgaa gcttcgccgg gcagagcgaa ttctggttcc agccggcgga
     3901 ctggcattga ttttgatttt gtctcaattc accggcgggc aaaaggattt cttctacttt
     3961 cagttctgag gtaatcatga agatcagctg gaaattatta ctcgctcctg ttttcgcact
     4021 cttcgtgcta tcctacgtag acaaagcgtt tcttttgcct gaagtgcgcg atcgattcct
     4081 ccagccaggc gggatggtat actacaagca gcgcgaaagg cagatcgatc gcatgcgcga
     4141 atatgcgaag gcaaaaccgc cggacattca tcttgcagtt gtactcggcg attcaagatc
     4201 atttgcgatt ggaaatttgc cggcgcagt
//
LOCUS       DAOQJL010000336         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12727_length_4220_cov_5.250900, whole genome shotgun sequence.
ACCESSION   DAOQJL010000336 DAOQJL010000000
VERSION     DAOQJL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12727_length_4220_cov_5.250900"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1042
                     /locus_tag="PLG78_13630"
     CDS             398..1042
                     /locus_tag="PLG78_13630"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE24208.1"
                     /translation="MVELYLQQFARQEADLMSPELAVFNTSRLQALWEAIIAVKGDKA
                     PVPFWCVVWPGARALARYILDHKDQFRGRRVLDAACGSGLAGIAAAKCGARVRGIDIS
                     PEAIELAKHMSHLNGTDCEWVVGDAFAESSELFDIVLVGDVFYDQALARKATKFMRSV
                     TDKGSVVLASDPGRNYCPREEYRAFGNLKVPVDPDLEGADFKITALLTMNRQAN"
     gene            1078..1383
                     /locus_tag="PLG78_13635"
     CDS             1078..1383
                     /locus_tag="PLG78_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24209.1"
                     /translation="MDQQVFYSLRDIPEGLDVIDELDLELKWETAIDPERSGAQSFTG
                     IEELNELLKERIRTLLIDKGGECVRVKYEKISHSKNYAHMSLIVVGEYHFEVLGKKK"
     gene            complement(1384..2469)
                     /locus_tag="PLG78_13640"
     CDS             complement(1384..2469)
                     /locus_tag="PLG78_13640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE24210.1"
                     /translation="MSSDARWVNDPDQLDQIIQKVFCKVQVILRHADQNMPVQVERLG
                     PGQLVLSHTASDAAARVIVARLQEGLIVLECVPDRRLPDGRELCKPRRLQVHRALRKE
                     PRTEIGPAKIFASSVVPSRQFPEAMAAGDVKRDNYLAAVEKDLRASLGEVSIILRKTI
                     RLDFRMKALQAGCRSILFSPEIISPDERFVPVKDYQQIITYDKLPKGVVSEISVGIYY
                     RKNYMYGFIRAFSPNSFSLAEFDHVSSVARKIEKFFEESNLLPSNPDKCPILDLSMSG
                     AGLLHPQSPIVMQKFMPGESVLLDISFPEGSLNFSASIRNIRSLENAHRIGIEFEGLD
                     SIRTAALEKALGPAVAASQAPGPSPGE"
     gene            2596..3285
                     /locus_tag="PLG78_13645"
     CDS             2596..3285
                     /locus_tag="PLG78_13645"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNE24211.1"
                     /translation="MKTVLVFAFLFVLQCAKAPEGAIISQVRGTASVEIQGKSEPATV
                     GKKVNAGDVIITEKDSEVDLQIAGGGAFRVLNASRITVGALAQKSEINVQKGGLLLGL
                     KKMKADEEFTVRSPTALAGVRGTSFSFSADRNTIAVLTGSVQVERQGKALQLEPFQEI
                     QTDKEKLESKKLSPDSARQLARIGEMKGIEDLADFDEIQKNLSVVILEIEKENLKTGK
                     TVAPRPRPEVQ"
     gene            3282..4205
                     /locus_tag="PLG78_13650"
     CDS             3282..4205
                     /locus_tag="PLG78_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24212.1"
                     /translation="MRSALFSLILLTACASAQDMFLQGVNAEQKNNSREALDYYAAAL
                     AKDPSMAKAYNNRGLILYKDKQYQDAIAEFDQALKIKPDYAAALSNRGLVLEAMNDPK
                     AAMAQYEKAVSLDPKFPEAYFNAGALSSKNGDILHAIQWLEKANELKADDGETLLLLG
                     RALLVAKNYGRAAQMFDALLKVDPNSRDAFIGSVDATRLAGNQDVAAARLNQYVAMHP
                     DCALCSSMLGAIYTDQKKYDSAIEVFEKSRQYRPGDPTPRYHLGFLYFLTKNKSKSIE
                     NLEEFLKLKGDFKDAQTEKASQMLAALKKGS"
BASE COUNT         1171 a         1023 c         1068 g          958 t
ORIGIN      
        1 cgtgcgtcga aaatggtgcg agccagtccg agtctgtaaa ccagagtcat caggcggcct
       61 tcgagtagca tcatgaagtt gtctgactta ttgcctttca ttctggatgc acgttcgaac
      121 gtgtggtaga attgcttttc catcaaacca taggtcagac gcagcttttg cttttcgagc
      181 agctgttccc cataaggcga tttcttcttc tttttgcgtg tatctgcatg gtgcccagga
      241 ggcgttggcc ttttgacgga agggcattta ggactgccgt aaaggcagta gccgacagtc
      301 cggcaaatct tgtgtctggg tttacgtatc gttgccaaaa taaaaactcc gagttagcat
      361 ggaaattttg agcgggtacc ctgtaaactg aaatcctatg gtagaactct acttacagca
      421 atttgcacgc caggaagcag atcttatgtc ccctgaactg gctgttttca atacctccag
      481 gctgcaagcg ctctgggaag caatcatcgc tgtaaaggga gacaaggctc ccgttccgtt
      541 ctggtgcgta gtctggccgg gagcacgcgc cctggcccgt tacattctgg accacaagga
      601 ccagtttcga gggcgacggg ttctggacgc tgcgtgcgga agcggtctgg cgggaatcgc
      661 ggcagcaaaa tgcggagcca gggtccgcgg aatcgacatc agtccagaag caatcgaact
      721 ggcaaagcac atgagccatc tgaatggaac cgattgcgaa tgggtggtgg gggacgcgtt
      781 cgccgaatcg tccgagctat ttgatattgt cctggtgggc gatgtctttt acgatcaggc
      841 ccttgcgcgc aaagctacga agttcatgcg aagcgtcacg gataaaggat cggtggtact
      901 ggcatcagat cccggcagaa actattgccc gcgcgaagag tatcgcgcat ttggaaatct
      961 aaaagtgccc gtcgatccgg atctggaagg agccgatttc aaaatcacag cactcttgac
     1021 catgaaccgc caggcgaatt gaattgattc tgcgcatgcc gcgccagatt ctgattcatg
     1081 gaccagcaag tattctactc actgcgagac atccccgaag gccttgatgt aatcgatgag
     1141 ctggacctcg aactgaaatg ggaaacggcg atcgacccgg agcgatccgg agcacagagc
     1201 ttcacaggca ttgaagagct caacgagttg ttgaaagagc gaattcgcac tctactgata
     1261 gacaaaggtg gagaatgcgt tcgtgtgaag tacgagaaaa tctcgcactc gaaaaactat
     1321 gcgcacatgt ctttgatcgt agttggtgaa taccatttcg aagtattggg aaaaaagaag
     1381 taactattct cccggagacg ggcccggggc ttgagatgca gcaaccgcag ggccaagagc
     1441 cttttccagc gccgccgtgc gaatggaatc caatccctca aattcaatcc caattctgtg
     1501 cgcgttttcg agagatcgaa tgtttcgaat cgatgctgaa aaattgagac tcccttctgg
     1561 aaagctgata tcgagcagaa cgctttcgcc cggcatgaat ttctgcatca caatcggaga
     1621 ctgcggatga agaagaccgg cgccagacat gctcaaatcc agaataggac atttatctgg
     1681 attggatgga agcaaattcg attcctcgaa gaatttctcg atctttcgcg ctacgctgct
     1741 tacatgatca aactctgcca gagaaaaaga gttaggagaa aatgctcgaa tgaaaccgta
     1801 catgtaattt tttctataat aaatcccaac tgatatttca gatacaactc ccttgggcaa
     1861 tttatcataa gttataattt gctgataatc cttgaccggg acaaaacgtt catcagggga
     1921 aataatctca ggagaaaaca aaatagatcg acatccggcc tgaagagctt tcatgcgaaa
     1981 atcaaggcga atggtcttac gaagaatgat ggagacttca cctagagaag ctcgcagatc
     2041 cttttccacg gccgccagat agttgtctct ctttacgtct ccggcagcca ttgcttccgg
     2101 aaactggcga gaagggacca cgctggatgc aaatattttc gcaggcccta tttcagtgcg
     2161 aggctctttt cgcaatgccc ggtgaacctg gagtcggcgc ggcttacaca gttccctgcc
     2221 atctggaagc ctgcggtcag ggacacattc cagaacaata agtccttcct gtaatcttgc
     2281 tacaatgact cgagccgcgg catccgatgc agtgtgcgaa agcacaagct gccctggacc
     2341 gagcctctct acctggacgg gcatattttg atctgcgtgc ctgagaatca cctgcacttt
     2401 gcagaacact ttctgaatga tttgatccag ctgatctgga tcatttaccc atcgggcatc
     2461 gctgctcaca aattccaggt aacaaatcta ccctggaagg gcaagcaatc catgcaattg
     2521 aaatgggaac caggatggta tgtccgctgg aaaaaattgc cttgtctggt ttgaatggcg
     2581 aacacagact gtatcatgaa gacagtcctt gtatttgcat tcctctttgt cttgcagtgc
     2641 gccaaagcac cggaaggtgc catcatttcg caggttcgcg gaactgcgtc tgtagaaatt
     2701 cagggaaaat cagaaccggc cacagtggga aagaaggtaa acgctggcga tgtaatcatt
     2761 actgaaaaag actccgaagt agatctgcaa attgccggtg gcggggcgtt tcgcgtactc
     2821 aatgcgtcgc ggattacagt gggtgctctc gcgcaaaaat cagagatcaa cgtacagaaa
     2881 ggcggactcc tacttggtct gaagaaaatg aaagcagatg aagagttcac ggttcgttcg
     2941 cccactgcac tcgccggagt gcgcggaacc tctttctcct tttccgcaga tcgaaacaca
     3001 atcgcagtcc tgacaggaag cgttcaagta gagcgtcagg ggaaagcgct gcaactcgaa
     3061 cccttccagg aaatccaaac agacaaagag aaactggaaa gtaaaaagct cagtccagac
     3121 agcgcgcggc agcttgcgcg aattggagaa atgaaaggca ttgaagatct ggcggacttc
     3181 gacgaaatcc agaaaaatct ttctgtagtc atactcgaaa ttgaaaaaga aaatctaaag
     3241 acaggcaaaa cagtcgctcc tcgaccaagg ccggaggttc agtgagatcc gcactctttt
     3301 cgcttattct tctaactgcg tgtgcaagcg cgcaggacat gtttctgcag ggcgtcaatg
     3361 cagagcaaaa aaacaattcc agagaggcgc ttgattacta tgctgcggct ctcgcaaaag
     3421 atccgtccat ggccaaggcc tacaacaacc gcggactgat actatataaa gataaacaat
     3481 atcaagacgc aattgctgaa ttcgatcaag cactcaaaat caaacctgat tatgctgcag
     3541 ctctgagcaa tcgaggtctt gtacttgagg caatgaatga tccaaaagcg gccatggcgc
     3601 aatacgaaaa agcagtctct ctggatccga aatttcccga agcctacttc aatgcagggg
     3661 cactttcctc aaaaaacgga gacattctcc acgcaattca gtggcttgag aaagcgaatg
     3721 aactaaaagc agatgacggc gaaacgctac ttttgcttgg ccgcgccttg ctcgtcgcaa
     3781 agaactacgg acgcgcagcg caaatgtttg atgcgctttt gaaggtagat ccaaacagcc
     3841 gcgatgcttt catcggaagc gtggatgcaa ccagactcgc cggaaatcag gatgtagcgg
     3901 cagccaggct gaatcaatat gtggccatgc atccggattg cgctctctgc tcaagcatgc
     3961 tgggcgccat ctacacagat caaaagaaat acgattcagc cattgaagta tttgaaaaat
     4021 cccggcagta tcgcccgggg gatccgactc cacgctacca ccttggcttc ctgtattttc
     4081 tgacaaagaa caagagcaaa tccattgaga atctcgaaga gttcttgaag ttgaagggag
     4141 atttcaagga cgcccaaaca gaaaaagcga gtcagatgct cgcagcgctc aaaaagggat
     4201 cctgatctca cagaatcgcc
//
LOCUS       DAOQJL010000337         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12827_length_4201_cov_4.588760, whole genome shotgun sequence.
ACCESSION   DAOQJL010000337 DAOQJL010000000
VERSION     DAOQJL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_4201_cov_4.588760"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLG78_13655"
     CDS             complement(<1..719)
                     /locus_tag="PLG78_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE24213.1"
                     /translation="MNQSRAMLATIPLAAALFLTSACKERKIISIDGSSTVGPISMAV
                     AEDFQHANGNVKVTVGISGTGGGFKKFCNGETDISDASRPIKKEEVEACKTKAIEYVE
                     LPVAYDGLAVLVSPQNDWVKELTTEQLKKIFTSENPAKTWKEVNPAWPDAAIKIFAPG
                     KDSGTHDYFVEEILGKKKQMRPDASFSESDNVLVTGISGEKNSIGFFGLAYFENNKDK
                     LKLVPIVNPKTKAAVLPSVDTV"
     gene            888..1487
                     /gene="phoU"
                     /locus_tag="PLG78_13660"
     CDS             888..1487
                     /gene="phoU"
                     /locus_tag="PLG78_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNE24214.1"
                     /translation="MLLDTKLKELRTEVAIMARRAEAMLEKATDLLHAPTEEGVKEVL
                     AMDATLNDLEIKMDDLCMRILALDEPYANDFRFVFSLIKTTRDLERIGDESKTVAKWS
                     QRITGEVPPRLLELGKKARETLNAAIHCLVNSDVSLTGTVMDLELTTDELEDEIINAN
                     PSIPIAFIAKALERVGDLSANIAENVVFSVKAEEIRHKT"
     gene            1539..3281
                     /locus_tag="PLG78_13665"
     CDS             1539..3281
                     /locus_tag="PLG78_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE24215.1"
                     /translation="MSRFRLLSYALTLFLLLSACTKNSSPRLIVVISIDQFRAEYLER
                     FKDLYLPAHSLGKPGGFRYLMEKGAYFRNAHHDHFPLYTAPGHSVLLTGAPPYKTGII
                     GNDWYDRKMGRKTYCVEDCNFKLIGVKNQSCIASDPAFKPGVSAKNLHASTVGDQLKL
                     ATGARAKVWGLALKDRAAVLLAGHAPDGVLWFDEDTGGWISSTAYFPNGKLPSWVTEW
                     NAKRIPDSYFNSKWELSVSRESLSRLWKSPFDHANSKAKLGTRFPHYLTAGLSEPDKS
                     FYTALTISPFGNEIVFDFAKELITREKMGQDSVPDLLAISLSSNDHAGHQYGPDSPEV
                     LDVAVRTDKLLSNFLIFLDQNIGLDNAIIVLSGDHGVLSNSRLLAEFGFPAGNYDERA
                     ICRSVLSDLRREFGPGDWIYGGDPSGPVRYETRSGCVEDSIFLNRELIARRNIPLELI
                     QSSAARSVSRWPGIYSAYSASDILKNRLPQNDIAARLMRSYYPPIGPDVIYIPEAFWS
                     SADYTLGATHGTPYSYDTHVPILMSGAGIAPGDHDERVSTLDIAPTLSGLLHILPPSG
                     SEGRILPVSLKTGR"
     gene            3339..>4201
                     /locus_tag="PLG78_13670"
     CDS             3339..>4201
                     /locus_tag="PLG78_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24216.1"
                     /translation="MTATLESERSERRTNLRPVFSAYELGKICIHARTLAQVTGSDFV
                     SETTPVVELYDLLSANPDLDFLPLTGPDGDVSGYIRRQMFFAKLSQTRFSRELLLRQD
                     VRAHSIMERRVICLDARTYLSEASEALMARDEEIRFDPFVVTYEGKFFGTATVQRVLE
                     GINHFLKLDLEACARMQDQILQPLERGFEITSDNHRIVMPLIAPGGDYAELIEINDRY
                     SLGLLFDVCGKGLKASSMVNTFASVIKTLIYKMDAEGSDLPRILLALHNSNQLLTQLT
                     CQEMYATGVAI"
BASE COUNT         1080 a         1051 c         1013 g         1057 t
ORIGIN      
        1 actgtgtcaa cagaaggaag aactgcagct ttggtctttg gatttacaat cggaacaagc
       61 ttgagtttgt ctttgttgtt ttcgaagtat gccagaccaa agaatccaat tgaattcttt
      121 tcgcctgaga ttccagttac cagaacattg tcagactcgc tgaaagaagc atccggacgc
      181 atttgtttct ttttcccgag gatttcttct acaaagtagt cgtgcgttcc tgagtctttt
      241 ccaggtgcga aaattttgat cgctgcatct ggccatgctg gattcacttc tttccatgtt
      301 tttgctggat tttcagaagt gaaaatcttc ttgagctgtt ccgttgtaag ttccttcacc
      361 caatcgttct gagggctaac aagaactgca agaccgtcat acgccactgg aagctcaacg
      421 tattcgatag ctttggtctt gcacgcttct acttcctctt tcttgatggg acgggaagca
      481 tctgaaatat cagtttcgcc gttgcagaat tttttaaatc cgccgccggt tccagaaatg
      541 ccaacagtta ctttcacatt tccgtttgcg tgctgaaagt cttcagcaac tgccattgaa
      601 ataggaccga ctgtgctcga tccgtcgata gagatgattt tcctttcttt gcaagccgac
      661 gtaagaaaga gagcggcggc gagcggaata gtagcgagca ttgctcgtga ttggttcatg
      721 gattcctcct gggatccggt tttgtcccgg ttaacatgaa cgtaatatcc aggagttatg
      781 ttacaattct atgacgctag aactacggaa gctggttttt ggacccttcc agagcagaat
      841 gggttgacct gtgaagaact gtaccgacag tcgaggcgga gtcatttatg cttctggaca
      901 caaaactcaa agagcttcgc acagaggtgg ccattatggc tcgccgggcg gaggcaatgc
      961 tcgaaaaggc gacggatctt ctgcacgccc ctactgaaga gggagtcaaa gaagtcctcg
     1021 ccatggacgc gactctcaac gatctcgaaa tcaaaatgga cgatctatgc atgcgaatcc
     1081 ttgctctgga cgaaccgtat gccaatgatt ttcgcttcgt attcagcctg atcaaaacaa
     1141 cgcgtgatct agaaagaatc ggcgatgagt ccaagacggt tgcgaaatgg tctcagagaa
     1201 ttacaggaga agttccgccg cgactcctgg aactcggaaa gaaggcaaga gagactctca
     1261 atgcagcgat tcactgcctc gtaaacagcg atgtttcgct gactggcact gtcatggatc
     1321 tggaacttac tacagatgaa ttggaagatg aaatcataaa tgcaaatcct tccattccca
     1381 tcgcattcat tgcaaaagcg ctcgaacgtg tgggagatct ctccgcgaac atagcagaga
     1441 acgtagtttt ctccgtaaaa gcggaagaga ttcgtcacaa aacatagaag tattccattc
     1501 gtcacagaac tggcacgaaa gcgattcatc ctctcaggat gagccggttt cgtttgctga
     1561 gctatgcact tacgcttttt ttacttcttt ctgcatgcac gaagaattcc agtccgcgcc
     1621 tgatcgtagt tatctcgatt gaccagtttc gcgcagaata cctcgagcga ttcaaagatc
     1681 tgtatcttcc ggcgcattct ctcggaaaac ccggcggctt tcgctatcta atggaaaagg
     1741 gtgcttactt tcgcaatgcg caccacgatc atttccctct ctatacagca ccgggacatt
     1801 ctgttttgct cacaggtgcg ccaccttaca aaactggaat cataggaaat gattggtacg
     1861 acaggaagat gggtagaaag acctactgtg tcgaagactg caacttcaaa ctcatcggcg
     1921 tgaagaacca atcttgcatc gcaagcgatc ctgcattcaa gccgggcgta tccgcaaaaa
     1981 accttcacgc aagcacggtc ggggatcaac taaagctggc cacgggtgcg cgcgcgaagg
     2041 tctgggggct tgcactcaag gacagagcgg ctgtacttct agccgggcat gctccggacg
     2101 gcgtattgtg gttcgacgaa gatactggcg ggtggatctc aagcaccgca tatttcccga
     2161 atggaaaact cccttcgtgg gtcacagagt ggaacgcaaa aagaattcca gattcgtatt
     2221 tcaattcaaa gtgggagcta tctgtttccc gggaaagtct ctctcgactc tggaaaagtc
     2281 catttgatca tgcaaacagc aaagcaaagc tgggtacgcg atttcctcac tatctgacag
     2341 ccggtctatc agagccggac aaaagttttt acactgctct aacaatttcc ccgtttggaa
     2401 atgaaattgt tttcgatttc gcgaaagaac tcatcacgcg ggaaaaaatg ggacaggact
     2461 ccgtaccgga tctccttgcc atcagcctct caagcaacga tcacgcaggg catcagtatg
     2521 gtccggacag tcccgaggtg ctcgatgtgg ctgttcgcac agacaaactt ctcagcaact
     2581 ttctgatttt tctagatcaa aacataggat tggacaatgc aatcattgta ctgtcaggag
     2641 atcacggagt gctcagcaat tccaggcttc tggcggagtt tggtttcccg gcgggcaact
     2701 acgatgagcg agcgatttgc agatcggtgc tcagtgatct tcgccgcgaa tttggcccgg
     2761 gagactggat ttatggcggg gatccttcag gtccggtgcg atacgaaacc aggagcggtt
     2821 gcgtagagga ttcgattttt ctgaaccgag agttgattgc gcgaagaaat attcccctgg
     2881 aactcattca atcttctgcg gcgcgaagcg tatcgcgctg gcctggaatc tactccgcat
     2941 actcggcctc agatattctg aaaaatagac taccgcaaaa cgatatcgct gcacgcctga
     3001 tgcgaagcta ttatcctccc ataggaccgg atgtgattta tattccggaa gcattttgga
     3061 gttccgcaga ctacactctg ggcgcgacac acggcactcc ttactcttac gacacgcatg
     3121 tgccaattct catgagcggc gcgggaattg ccccgggcga tcatgatgaa agagtctcca
     3181 cactcgacat tgcgccgact ctctcgggat tgctgcacat cctgcctcct tccggttcag
     3241 aaggccgcat tctgccagtc agtctcaaga ctggcagatg aacctgacat tcacacctat
     3301 gacacggaac agtaacgcgt caggatcata ctgttcctat gacagcaact ctggaatcag
     3361 aaagatcgga acgtcgcacc aatcttcgtc ccgttttctc cgcatacgaa ctgggaaaga
     3421 tctgcattca tgcaaggacg ctggcgcaag tgactggcag cgattttgtc tcagaaacaa
     3481 cgccagtcgt tgaactatat gatctactga gcgcgaaccc ggatctagac tttcttcctt
     3541 taacaggacc ggacggagat gtctccggct atattcgcag acaaatgttt tttgccaaac
     3601 tcagccagac caggttttcg cgcgaactat tgctcaggca ggatgtgcgc gcgcatagca
     3661 tcatggagcg ccgcgtaatc tgtctggatg cacggaccta cctttcagaa gcctcagaag
     3721 cgctcatggc acgcgatgaa gaaatccgat ttgatccgtt cgtcgtcaca tacgaaggaa
     3781 aatttttcgg cacggcaacc gtgcagagag ttctagaagg tatcaatcat ttcctcaagc
     3841 ttgatctcga agcatgcgcg aggatgcaag atcaaatcct ccaacccctg gaacgtggat
     3901 ttgaaatcac atctgacaat catagaatcg tcatgcccct cattgcacca ggcggcgact
     3961 atgcggagct catagaaatc aacgatcgtt attcactggg gcttctattc gatgtgtgcg
     4021 gaaaaggact caaagcttct tccatggtaa acacgtttgc atctgtgatc aagactctga
     4081 tctacaagat ggacgcagaa ggcagcgatc ttcctcgcat cctacttgcc cttcataact
     4141 ctaaccagct gctcacgcaa ctcacctgtc aggagatgta tgcaacgggt gttgccatca
     4201 t
//
LOCUS       DAOQJL010000338         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_12868_length_4194_cov_4.188935, whole genome shotgun sequence.
ACCESSION   DAOQJL010000338 DAOQJL010000000
VERSION     DAOQJL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_4194_cov_4.188935"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLG78_13675"
     CDS             <1..266
                     /locus_tag="PLG78_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24217.1"
                     /translation="HRFLSHAVFEEIQSEEENLEMQCCLLHEPPFRRLPFRKKKPDEK
                     KPAWLLDEQFALESEVNRFHLVERRQNESAPEGVFLDYPNRLG"
     gene            complement(281..2113)
                     /gene="glmS"
                     /locus_tag="PLG78_13680"
     CDS             complement(281..2113)
                     /gene="glmS"
                     /locus_tag="PLG78_13680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNE24218.1"
                     /translation="MCGIVAYLGPKSVDSVLLVGLTRLEYRGYDSAGIAVLDNGEITV
                     RKEKGKIKDLEDLLLTHPVEGTIGIGHTRWATHGEPNRQNAHPHVDNHGKVAVVHNGI
                     IENHHTIRQELIEKGHVFHSETDTEVIPHLFEDYYKKHKDSAKALQETIARLDGRYAF
                     VVLVEQEPDRLYFARSGSPLVYGRGRGESFLASDVSAIVPAAKEHLHIDNDQWGWITK
                     DSLELRSKTGEAVPFEMSRTQIDVKALDRAGYDHFMLKEIHEQPGIVRRIIQSRMDDE
                     GIVRFPEMNLPNDFLNRVTRCMITSAGTSWHAGIVGKFYLEQFARLSTEVDISSEFRY
                     RNPIADGETLVMAISQSGETADTLAGIHQAKAKFCRVLSFVNVTTSSIARESNAFIDL
                     MAGPEIGVASTKAYTAQLMNLLFYALFTAGIKWLVSKEEREQILHEIRLLPEKMEQIL
                     ARADEIKKWASELRDSKDFVFLGRSFNHPTALEGALKLKEIAYVHASGYAGGEFKHGP
                     IALISDQMPVICIAPQCDLYEKMVSNIEEVRSRKGRIISIYTEGDTIVPSKSDYSFAI
                     PQCADYLTPILTVLPLQLFAYYSALERGCEPDQPRNLAKSVTVE"
     gene            complement(2218..3135)
                     /locus_tag="PLG78_13685"
     CDS             complement(2218..3135)
                     /locus_tag="PLG78_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24219.1"
                     /translation="MRRTVAILASVGIVLAVLPFACDGERHAITEQDRAGKPGQFAGL
                     SQGVTHYEIAGPAKGRPVLLVHGFSVPYFIYDFVFEALVQKGFRVIRLDLYGRGLSDR
                     PDVVYDLDLYDRQLTDLLDYLKISRVHIVGSSMGGLITANFLRKHPERVSRAVFIAPA
                     GFPMPLPISAKIGRAPYIGDYIVRVAGDRIFVKNNSASFFGNVPPGFYERFGEQMQFK
                     GYKRALLSSLRNMPLESGASIFDEAGRKKIPSIVIWSRQDGVLPYSNAIRAQYSLGAS
                     ELISLEGTGHTPHFENPQMVIPHILKFFQ"
     gene            complement(3132..3623)
                     /gene="purE"
                     /locus_tag="PLG78_13690"
     CDS             complement(3132..3623)
                     /gene="purE"
                     /locus_tag="PLG78_13690"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNE24220.1"
                     /translation="MNQPVVSIIMGSASDRSVMEQAAVALKEFSIPYEMTIVSAHRTP
                     DRMVEFAASARKRGIRVIIAGAGGAAHLPGMVASLTELPVIGVPILSSNSLDGWDSLL
                     SIVQMPAGVPVAAVAVNGAKNAGVLAARILGAGDPDLLDRMRTFMNQQRDVVLESARE
                     MEQ"
     gene            complement(3620..>4194)
                     /locus_tag="PLG78_13695"
     CDS             complement(3620..>4194)
                     /locus_tag="PLG78_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE24221.1"
                     /translation="VIVSRNEFGQMAAFPAVEMVPTPAHVLDYLLSPARITEDQHRRA
                     NEIAFEIAELIGLVGILAVEMFISNDQVLVNEIAPRPHNSGHATIEANMTSQYEQHLR
                     MILGHPPGPTDSLSSSALLNLLGQGDGTPLIHGMREAMGIPGVSVHMYGKRVCKPGRK
                     MGHVTVLAPDRSILLERIELVRSVLRVGPQ"
BASE COUNT         1081 a         1082 c         1018 g         1013 t
ORIGIN      
        1 tgcaccgttt tctatctcat gcggtgttcg aggaaattca gagcgaagag gagaatcttg
       61 aaatgcaatg ctgtctgctc cacgagcctc catttcgcag actgcctttt cgtaagaaaa
      121 agccggatga aaaaaaacca gcctggctgt tggatgagca attcgcgctc gagtcagagg
      181 tgaatagatt ccatctcgta gagagaagac agaacgaaag cgcgccagag ggagtgtttc
      241 tggactaccc gaatcgatta ggataacgta agatatggtc ttactccacc gtaacagact
      301 tggcgaggtt gcgcggctga tcaggctcgc acccgcgctc gagcgcagaa taatatgcga
      361 acagttgcaa tggcaatact gtcaggattg gagtgaggta atccgcgcat tgtggaatcg
      421 caaatgaata atcagacttc gaaggaacaa tcgtgtcccc ttctgtgtaa atggaaatga
      481 tgcggccttt cctcgaacga acttcttcaa tatttgaaac cattttttcg taaaggtcac
      541 actgtggtgc aatgcagatc acaggcatct ggtctgaaat caaagcgata ggtccatgct
      601 tgaactctcc gcccgcatat cctgatgcat gcacatatgc aatttctttg agtttcagtg
      661 ccccttcgag agcagtcggg tggttgaacg atcggcccag gaaaacaaag tctttggaat
      721 cacgaagttc gctcgcccat ttcttgattt catctgcacg cgccagaatc tgttccatct
      781 tctcaggaag gagacggatc tcgtgcagga tctgctcacg ttcttccttg ctcacgagcc
      841 actttatgcc tgcagtgaag agagcataga atagaagatt catcaattgc gccgtatatg
      901 ccttggtaga ggccacaccg atctccggac cagccatcaa atcaatgaac gcattggatt
      961 cgcgcgcaat gctagatgtg gttacattga caaaagatag aactcgacag aactttgctt
     1021 ttgcttgatg aatgccggct aacgtgtctg ctgtctcccc gctctgagat atggccatca
     1081 cgagggtttc cccatccgca atcgggttgc gatatctaaa ttcagaagaa atatccactt
     1141 cagtggaaag cctcgcaaac tgctccagat agaattttcc tactatcccc gcatgccagg
     1201 acgttccagc ggaagtaatc atgcagcgcg taacacgatt gagaaaatcg tttggtaaat
     1261 tcatttcagg gaatcgaaca attccctcat cgtccatgcg actttgaata attcttctta
     1321 caatgccggg ctgctcatgg atttccttga gcatgaaatg gtcgtatcca gcacgatcga
     1381 gtgctttgac atcgatctga gttctggaca tttcaaacgg aactgcctca cctgttttgc
     1441 tacgaagttc aagactatct tttgtaatcc agccccactg atcattgtct atgtggaggt
     1501 gttctttggc tgccggcaca atggcggaca cgtcgcttgc aagaaaactc tctcctcttc
     1561 cgcgcccata gacaagagga gaaccgcttc gtgcgaaata gagacgatcc ggctcttgct
     1621 ctaccagtac cacgaatgcg taccggccat ccaggcgcgc gatggtttcc tgaagagcct
     1681 tcgcagaatc cttgtgcttc ttgtaataat cttcaaatag atgaggaatc acttctgtgt
     1741 ctgtctcaga atgaaagacg tgaccctttt caatcagctc ctgacgaatc gtgtgatgat
     1801 tctctatgat tccattgtga acgactgcca cttttccgtg attgtcaaca tgcggatgcg
     1861 cattctggcg attgggttct ccgtgcgtag cccagcgagt gtgtccaatt ccaatggtcc
     1921 cttccactgg atgcgtgagc agcaaatctt caagatcttt gatctttcct ttttcttttc
     1981 tgaccgtgat ttcaccgtta tccagaacgg cgatgccggc ggaatcgtaa ccccggtatt
     2041 caagacgcgt tagaccgact agaagaaccg agtcgactga cttcggtccc agatacgcta
     2101 caataccgca catgagtgaa acaaaattag aaggactccg tgaagcaagt ccgattcaag
     2161 ggagcgatct tctcgagtcg ttgcgggtct gcaaccatgt ggagaaattt caacgtgcta
     2221 ctggaagaac ttcaggatgt gcggaatcac catctgtgga ttttcaaagt gaggcgtgtg
     2281 ccccgtgccc tcgagcgaaa taagttctga cgctccgagc gaatactgtg cgcgaatcgc
     2341 gttggaatag ggaagaactc catcctgcct ggaccagata acgatagacg gaatcttctt
     2401 tcgccctgct tcatcgaaaa tggacgctcc actttccagt ggcatgtttc gcagggaaga
     2461 cagcagagct ctcttgtatc ctttgaattg catctgttct ccaaaccttt cgtaaaaacc
     2521 aggaggcaca ttgccaaaaa aacttgcact gttgttcttt acgaagatgc gatctccagc
     2581 cacgcgcaca atgtaatctc caatataggg cgcgcggcca attttcgcgc taataggaag
     2641 tggcatagga aagcccgcag gcgcaatgaa caccgcgcgg gaaactctct ctggatgctt
     2701 tcttaagaag ttcgcggtga tgagtccccc catggaagat cctacaatgt gaacgcgcga
     2761 aatcttgaga taatccagga gatccgtcag ctgcctgtca tagagatcaa gatcatagac
     2821 aacgtcaggg cgatccgaaa gtccgcgccc gtacaggtcc aggcggatca cacgaaatcc
     2881 tttttgaaca agagcttcaa aaacaaaatc atagatgaaa taaggaactg aaaagccgtg
     2941 aaccagaagc accggacgcc ccttcgccgg cccggctatt tcgtagtgag ttactccctg
     3001 cgaaaggccg gcaaattgac cgggctttcc cgctcgatcc tgttcagtga tcgcatgacg
     3061 ttctccgtca caggcaaaag gcaatacggc cagaacgatt cccacagaag ctagaatcgc
     3121 aacggtccgc ctcactgttc catctcccgg gcagattcga gaaccacgtc acgctgctgg
     3181 ttcatgaatg ttcgcatccg gtcgagtaaa tcaggatcac ccgcgccaag aatacgagcc
     3241 gcaagaacgc ctgcattctt tgctccgttc actgcaacag ctgccactgg aacgcccgct
     3301 ggcatttgca caatagatag taaagaatcc caaccatcca gtgaattgga ggaaaggatg
     3361 ggcacgccaa tcacgggtag ctctgtcaga gacgcgacca ttcctggcaa atgtgcggct
     3421 ccgcccgctc cggctatgat cacgcgaatc cctctctttc gcgcagaggc agcgaattcg
     3481 accattcgat ctggagtgcg atgagcagag acgatggtca tttcgtaagg aatagaaaac
     3541 tctttgagcg cgacggcggc ctgttccatg acagaacgat ccgatgcact gcccatgatg
     3601 atagaaacta ctggctgatt cattgcggcc ccacacggag cacactgcga accaattcga
     3661 ttctctcaag caaaatagat cgatctggag caagaaccgt aacgtgcccc atctttcttc
     3721 ccggtttgca cactcgtttg ccatacatgt gcacggacac acctggaatg cccatcgctt
     3781 ctcgcattcc atgtatgaga ggcgttccgt ctccctgacc gagcagattc aaaagagcac
     3841 tcgaagacag ggaatcggta ggacctggcg gatgacccag gatcatgcgc agatgttgct
     3901 cgtactgcga agtcatgttt gcttctatgg tcgcatggcc gctgttgtga ggacgcggcg
     3961 cgatttcatt gaccagcact tgatcattgg aaataaacat ctccactgca agaattccga
     4021 caagtccaat gagttccgca atttcaaagg cgatctcatt ggcgcgtctg tgttgatctt
     4081 ctgtgattcg agctggactg agtaaatagt cgagcacgtg tgctggagtg ggaaccattt
     4141 ccacggctgg aaaagctgcc atctgcccaa actcattgcg actgacgatg acag
//
LOCUS       DAOQJL010000339         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13021_length_4161_cov_3.146615, whole genome shotgun sequence.
ACCESSION   DAOQJL010000339 DAOQJL010000000
VERSION     DAOQJL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_4161_cov_3.146615"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLG78_13700"
     CDS             complement(<1..987)
                     /locus_tag="PLG78_13700"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE24222.1"
                     /translation="MDALGAFRIPTFRNYLLAKLLFVLGAQVQIAAVRWQVYSLTKDP
                     LALAWIGLIEAIAFIAVALFGGHLADMIRPGKIILACLSILAGCSGCLLLLSGPGSFL
                     LVSVTPIYAVIAVSGAARGILSPALFAFMTALIPPRLYASSATWSSGSWQMAAVAGPA
                     IGGLLYGAAGPIVAYTADITLVLGAIVFAGISIRTAPEQTLHPVTEGLSERLTGGVRF
                     VFGNQLLLGAMSLDLFAVLFGGAAAMIPLFADRLETGAQGMGFLMSAPAAGAVAMSVG
                     LAFAPLGRRAGWVLLSATAGFGLCMIGFALSSTFVLSMVFLVCSGAMDQVSVV"
     gene            1100..1414
                     /locus_tag="PLG78_13705"
     CDS             1100..1414
                     /locus_tag="PLG78_13705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE24223.1"
                     /translation="MDERRRFHRISSQFSLEVHPSESGEGTSQNVSATGLLFSQKSPS
                     PIGTTLYLTIRQPGLSGDVSVKGRVVRCEPIGSQFQIAVQFVDVDKETEDSILDLVKE
                     SG"
     gene            complement(1443..1919)
                     /locus_tag="PLG78_13710"
     CDS             complement(1443..1919)
                     /locus_tag="PLG78_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24224.1"
                     /translation="MRSMFLIAALFALASCAGTADKVSGGEEIEGWRTDQATGVDTFF
                     MRVPGRATNNAIEQDSPTMMKSTCIDSTKLQALDNIIRKMIGETVTGQSATLDGQSTG
                     VLITSVRGGLIRGTQMGQCAPVDKSKKYESCECIHYVTGKGLKKKFELAVEQAAKK"
     gene            complement(1967..4000)
                     /locus_tag="PLG78_13715"
     CDS             complement(1967..4000)
                     /locus_tag="PLG78_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE24225.1"
                     /translation="MTESAKEPAATHAPWQREFFKNIEGFMQVGIPEDKAKEILMTFL
                     KLSSSTPMPRVMESFRSPEILEEVGVHTKQNPELRDFMATFLDPVMRNFTVEGLENLS
                     GILPLLGKYPMTLISNHLSHLDAPAIYNLLYRQGGDARKLADSLVFIAGRLAFEPDFT
                     RLGLYMFDTLLVCSKLDMTENPGLADLMTRINMRAFRQANQLQKEGKIIAIFPEGTRS
                     RTGKLVNFVDTVYHYVANKIIVPVSLAGTEEILPTQSFLFNASKGKLVLGKPVLVGDL
                     PKSQMASLPEYVQRLAIPEVGDKKQFVIDNLALLIGQNLHKHRHGTYRNLYRGDDLGS
                     NVLITRPKTPTERVVVLGHSPYGTAAATILANKNVSVQIYLDDEAKVAEFNEKSVDLD
                     HFPLFKLPPNISYTANPAEVESGTLFVQAARSWELDRIYSRVKLYLQKANGPILNIVK
                     GFTGSKYGLILDDLEHFYELDPKRFVAIAGANYPEQVMERKLTGYEVAANNPDLIDHI
                     CKLFSTGYVFTRPAVVSDDVRGVQLGGALKNIYALGIGLLDGYYEKSLGGNTDNSLFH
                     VSNRIFKEMKIIGSKLGGKPGTFEGLSGLTDLMMACFGQDSRDRQYGHDYIYGKANPD
                     QKTAGLFGVRSLPNLLPLNPEEYPVASAVHAVLVQKRDYDSVLNEIMSKLKRF"
BASE COUNT         1018 a         1104 c         1049 g          990 t
ORIGIN      
        1 tacaacgctt acttgatcca tcgcaccgct gcaaaccaga aacaccatag acaaaacaaa
       61 cgtgctggaa agagcaaatc caatcataca gagtccaaac ccagccgtcg cactgagaag
      121 cacccagcct gcgcgtcttc ccagcggagc aaacgcaagg cctacggaca tggccacagc
      181 acctgccgct ggcgcgctca tcaaaaagcc catgccctgc gctcccgtct caagtcgatc
      241 tgcgaaaaga ggaatcatgg ctgcggctcc tccgaacaac actgcaaaca aatccaggct
      301 catcgctccg agcaaaagct ggttgccaaa gacaaatcgc actccgcctg tcagtctttc
      361 gctgagtcct tccgttacag gatggagtgt ttgttccggt gctgtgcgaa tactgatacc
      421 cgcaaaaaca atggccccga gtacaagagt gatgtctgcc gtgtatgcga cgatgggacc
      481 ggctgctccg tacagcaaac ctccaatggc gggaccggcc actgcggcca tctgccagct
      541 accactgctc caggtcgcag agctcgcata caatctgggt ggaatgaggg cagtcatgaa
      601 tgcgaagaga gcaggcgaaa ggattccgcg agccgccccg ctcactgcga tgactgcgta
      661 aattggcgtc acgcttacga gcagaaacga accaggacct gaaagaagaa ggaggcaacc
      721 tgagcaaccg gcaaggatcg atagacaggc cagaatgatt ttgcccgggc gaatcatatc
      781 tgccagatgc ccaccaaaca aagccacggc gatgaacgcg atcgcctcta tgagtccgat
      841 ccaggccagg gcaagcggat cctttgtaag actgtacacc tgccatctca ccgccgcgat
      901 ttgaacctgc gcgcccagca cgaacaacag cttcgccaga agatagttgc gaaacgttgg
      961 aatgcgaaag gctcccaggg cgtccattct accagggaac cggaaggctc cctggcgccg
     1021 aagaaaaatt caagtttccg acatccgttt taaaaaagac ttgaaactca aggcgactgc
     1081 cgcaagacta ctggtcgata tggacgaacg cagaaggttt catagaatca gctctcagtt
     1141 ctcactggag gttcatcctt cagaatcagg agagggcacc agccagaatg taagcgcgac
     1201 gggtctgctg ttttcgcaga agtcaccatc gcccattggg accactctgt atcttaccat
     1261 ccgccagcca ggtctgtccg gggatgtgag tgtgaagggc cgcgtcgttc gttgcgaacc
     1321 aatcggtagc cagtttcaaa ttgcagtgca gtttgtagac gtagacaagg aaacggaaga
     1381 cagcattctg gacctcgtga aagagtcagg ctaggcccag ggcccagccg ctctttttta
     1441 gattacttct tcgctgcctg ttccacagcc agctcaaact tctttttcaa gcctttgcca
     1501 gttacgtagt ggatgcactc acagctttcg tatttctttg atttgtccac aggggcacat
     1561 tgtcccatct gcgttccacg aatcaagcca cctcttacag aagtgatcaa tacgcctgtg
     1621 gattgtccgt cgagcgttgc agactgaccg gtgactgttt ctccgatcat tttgcgaatg
     1681 atgttgtcga gcgcctgcag cttcgtggaa tcaatgcaag tggacttcat catggtggga
     1741 ctgtcctgtt caattgcgtt gttcgttgca cgaccgggca ctctcatgaa gaacgtgtct
     1801 actcctgtgg cctggtctgt acgccagcct tcaatttctt ctccgccaga aactttgtct
     1861 gcggttccgg cgcacgatgc cagagcaaac agcgctgcga ttagaaacat ggatctcatt
     1921 ttgttctcct gaatgtattg gacgaatctt aactctgcga gttcggttaa aaacgtttca
     1981 gctttgacat gatttcattt agaactgaat cgtaatccct cttctgcaca agaacagcat
     2041 gtactgcaga ggcaaccgga tactcctctg gattgagagg aagcaggttg ggtagagatc
     2101 gcactccgaa tagaccggcc gttttctgat ctggattggc ctttccgtaa atgtaatcgt
     2161 gcccatactg gcgatcgcgc gaatcctggc caaagcacgc catcatcaaa tctgtgaggc
     2221 cggaaaggcc ctcaaatgtt ccaggctttc cgcccagctt gcttccaatg attttcattt
     2281 ctttgaagat tcgattggat acgtggaata aggagttgtc tgtgtttccc ccgaggctct
     2341 tttcataata gccgtctaga agtccaattc caagagcata tatattcttc aatgctccac
     2401 cgagttgaac tcctcttacg tcatcagaaa ccacagccgg gcgagtgaaa acatatccgg
     2461 tgctgaacag tttgcaaatg tgatcgatca aatctggatt gttggccgcc acttcgtatc
     2521 ctgtgagctt tcgctccatc acttgttcag gatagtttgc gccagcgatc gccacaaacc
     2581 tcttcgggtc gagttcatag aaatgttcca gatcgtctag aatcaggccg tattttgagc
     2641 ctgtaaatcc tttgacaatg ttcaggatgg gcccgtttgc cttttggaga taaagcttca
     2701 ctcgcgaata gattcgatcg agctcccagg atctggctgc ctgcacaaac agagttccgg
     2761 attccacctc cgccgggttg gccgtgtaag agatgttagg cggaagtttg aaaagcggga
     2821 agtgatccag gtcgacgctc ttctcgttga attcagcaac tttcgcttca tcgtccagat
     2881 agatctggac actcacattc ttgtttgcaa gaatcgtggc cgcagctgtt ccgtacggcg
     2941 aatgtcccag cacgaccact ctctcagtcg gagtttttgg tcttgtgatg agaacattgc
     3001 tacccagatc atctccgcga tagagattcc tgtatgtgcc atgcctgtgt ttgtgcaggt
     3061 tctgtccgat gagcagcgcc aggttatcaa tcacaaactg ctttttgtct ccgacttcgg
     3121 gaatagccag tctctgtacg tattccggca gggaagccat ttgcgatttg ggaaggtctc
     3181 cgactaacac tggtttgccg agcaccagct tccctttgct tgcattgaaa agaaaagact
     3241 gggttggaag aatttcctcc gtccctgcga gagacacagg cacgatgatt ttgtttgcga
     3301 cgtagtgata aactgtatct acaaaattga ccagctttcc tgttctactg cgagtccctt
     3361 ccgggaagat cgcaatgatc tttccctctt tctgcagctg gtttgcctga cgaaacgcgc
     3421 gcatgttgat gcgagtcatc aagtctgcga ggcctggatt ctcagtcatg tccagtttgg
     3481 aacaaaccag aagagtatcg aacatataca gaccaaggcg agtgaagtcc ggttcaaaag
     3541 caagtctgcc ggcgatgaaa accaggctgt ctgcgagctt gcgcgcatct cctccctggc
     3601 gataaagcaa attgtaaata gcaggcgcgt ccaggtgact gaggtgattg gaaatgaggg
     3661 tcattggata cttgccgaga aggggcagga tgccggatag attctcaaga ccttccactg
     3721 taaagttgcg catgactgga tccaggaacg tggccatgaa atcgcgcagt tctggattct
     3781 gcttcgtgtg aacacccact tcctccagga tctccggact gcgaaagctc tccataactc
     3841 tgggcatggg tgtgcttgat gagagcttca ggaaggtcat caggatttcc tttgctttgt
     3901 cctcaggaat ccccacctgc atgaatccct ctatgttctt gaaaaattcc ctctgccacg
     3961 gggcatgggt ggcggccggc tcttttgcgc tctcagtcat ggaaggtgtc tgacctggca
     4021 gtaccccgcg tgcaagcaaa tttcattgac agggcactgc gtgccgaagc tataaagaag
     4081 agtcatggcc aggaccccgg aaaagaaaaa gaagcccgcg aagaaggcaa aacgtccacc
     4141 aaacgccgcg atcgtgatgg c
//
LOCUS       DAOQJL010000340         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13043_length_4157_cov_4.230619, whole genome shotgun sequence.
ACCESSION   DAOQJL010000340 DAOQJL010000000
VERSION     DAOQJL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13043_length_4157_cov_4.230619"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1049)
                     /locus_tag="PLG78_13720"
     CDS             complement(63..1049)
                     /locus_tag="PLG78_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNE24226.1"
                     /translation="MHLILDSIIYQLQAHGGITQFWQNLCSRMKSYPDLDTEEIAGHK
                     LLRYNPLWMRCDVFHSSYYRLNFWPGVRNVVTVYDFLYERGHVESRFRHLHIWQKRAA
                     IRRADAVVCISENTRQDLLRFYPDAENRASVVHLAAGHSEPGAPGRATAHIETRPFFL
                     FVGHRYGYKQFELVVQAMTEARLFDQYDLVCTGSPFSEAEQKSFSDNQIATYVKSIPG
                     ATPGEMQALYAHAKALVYPSLYEGFGLPVVEAMTAGCPVVACDSSSIPEVAGNAALLF
                     LPGDLPGLVRALEQVCQKGARERYAALGQIQAEKFSWEKTVAGYHKIYQALK"
     gene            complement(1049..1825)
                     /locus_tag="PLG78_13725"
     CDS             complement(1049..1825)
                     /locus_tag="PLG78_13725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE24227.1"
                     /translation="MNPVCRVCGEATHSFGEALLLAKHRVHYFECDVCGYVQTEDPYW
                     LEEAYQNPVNLTDTGLLGRNVMLSRMTLLLLRRYFKKGDFVLDYSGGYGVLTRLLRDA
                     GAECYWHDPYTPNVFARGFEMHRAARSFSLVTSFESFEHFVHPLEEIRKISQISSEIF
                     FSTRLLPPHRPQPGQWWYYGLEHGQHIGFFTPASLAHIAKELGLHVVSAFGAYHFLSR
                     RKIHPLSFAVTLAFSRTPFVSLFHPGPSLTVRDSEFLSRI"
     gene            complement(1822..2715)
                     /locus_tag="PLG78_13730"
     CDS             complement(1822..2715)
                     /locus_tag="PLG78_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNE24228.1"
                     /translation="MSLETPVVFFAFNRPDVTRRVFERIAAQKPARLFLIADGARNAT
                     ESKLCEEVRSIITNVNWSCDVQTNFATENMGCRARLSSGISWALERSEEVIILEDDCL
                     PHPDFFRFCSELLARYRTDERVFSISGDNFQPESRGYSYYFSRYPHVWGWATWRRAWQ
                     KYDVDLASWPELSRNHSFRELVKGPAGRYWARNFDLICKGFNTWDIQWTFACLLNQGL
                     TILPDKNLVSNIGFGAEATHTKRKSPLEALGTTELDWPLRHPPFVARNEAADEFTEHR
                     VFRVRPVDRLIYLARQLWRYL"
     gene            complement(2712..3548)
                     /locus_tag="PLG78_13735"
     CDS             complement(2712..3548)
                     /locus_tag="PLG78_13735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE24229.1"
                     /translation="MVHIILPVHNRIELTRAFVESLVQSSSKDFHIVLVDDGSTDGTS
                     EMVCESFPFTTVIRGTGNWWWGGSLHQGYLWARSAELPTSDVIMTVNDDTALDPAFLE
                     NAERLLKERPRDLLVATARDGRDILQEQRMKVDWLRLRFRPIRDGETPDWCTTRGLCM
                     RVSEFLSIGGFYPTLLPHYLSDVEYTLRARRKGHPIFSDPSVSLRMNTESTGHHLLKS
                     MRFWPYVKTVFSRKTSHNPIYMTTFLFLAAPFWAIPTGLLKVWALPLVEFFGFRREGE
                     IA"
     gene            complement(3565..>4157)
                     /locus_tag="PLG78_13740"
     CDS             complement(3565..>4157)
                     /locus_tag="PLG78_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE24230.1"
                     /translation="GYTISRKPRWKVSAEKRILKVESRKIHYGSGGRLMSGWVNVDLY
                     PSESLIADPVRVSANLTLEHPFADGWFLYAFSEDFFEHLEQKQQLLFLVEAYRVLAPG
                     GVLRMSFPGLEGVLMRHFSDNRLASYGQGVYDSYDVFEHKHFASRAELELMCRHIGFS
                     SVDFEIKYGESRHEPLKGLDSRVEQIGLNTYAEITK"
BASE COUNT         1129 a         1073 c         1016 g          939 t
ORIGIN      
        1 cgtatctcgt atggtcgctt cgctgttaaa gcagacagtt acaatgctca ccgaaggctt
       61 attcatttaa gcgcctgata gattttatga tagccagcga ccgttttctc ccatgagaat
      121 ttctcggcct ggatttgacc cagtgccgca taacgctcgc gcgcaccttt ctgacaaact
      181 tgctcgagcg cacgcacaag tccaggtaga tctccaggca agaacaacaa agctgcattg
      241 cccgccactt ccggaatcga agaagaatcg catgcaacca cagggcaccc tgcagtcatt
      301 gcctctacga ccggcaatcc gaaaccttcg tagagagaag gatacaccag agctttcgca
      361 tgagcgtaca atgcttgcat ttcgcctggt gttgcccccg ggatactctt cacatacgtt
      421 gcgatttggt tgtctgagaa actcttctgt tctgcctcag agaaaggaga gccggtgcaa
      481 acaaggtcgt attggtcaaa caggcgcgcc tctgtcatgg cctgaaccac gagctcgaac
      541 tgtttgtatc cgtaacgatg tcctacaaaa aggaaaaagg gcctggtttc tatgtgcgcg
      601 gtcgcacgcc cgggtgcacc tggttcagaa tgtcccgcag ccagatgaac cacgctcgca
      661 cgattctctg cgtctggata aaatcgtaga agatcctggc gggtgttttc agatatacag
      721 accactgcgt ccgctcttcg aatggcagcg cgcttctgcc agatatgaag atgcctgaag
      781 cgggattcaa cgtgccctct ctcatagaga aaatcataca cggtcacaac gttgcgcacg
      841 ccgggccaga aattcaaacg atagtagctg gaatgaaaaa cgtcgcagcg catccacaaa
      901 ggattgtagc gcaaaagttt atgacccgct atttcttctg tgtccagatc cgggtacgat
      961 ttcatgcgcg agcacaaatt ctgccagaac tgagtgatgc cgccgtgggc ttgcagttga
     1021 tagatgatgc tgtcgagtat aagatgcatt aaattcgact cagaaactca gagtctctca
     1081 cggttagact ggggcctgga tgaaacagag acacgaaagg cgtacgcgag aaggcaagag
     1141 taacggcaaa ggatagagga tgaatcttac ggcgcgacag aaaatggtag gcaccaaacg
     1201 cagaaaccac gtggagaccc agctctttcg caatgtgtgc gagagatgca ggagtaaaga
     1261 agccaatgtg ctgcccatgc tccaaaccat agtaccacca ctgtccgggt tgtggcctgt
     1321 gaggtggaag tagcctcgta gagaaaaaaa tctccgacga aatctgcgaa atcttgcgga
     1381 tctcttcaag aggatgaaca aaatgttcaa aactttcaaa gcttgtcacc agagaaaacg
     1441 atctggcggc gcggtgcatt tcaaagccgc gagcgaagac attgggagta tacggatcgt
     1501 gccagtaaca ctctgccccg gcatcgcgca acagccgcgt tagaacgcca tagcctcccg
     1561 aataatccag aacaaagtcc cctttcttaa agtaacgacg caatagcaac agagtcatgc
     1621 gcgaaagcat tacgttacgc ccgagaagac ctgtgtcggt gagattcact ggattctgat
     1681 acgcttcttc caaccagtat ggatcttccg tttgaacata tccacataca tcacattcga
     1741 agtagtgtac gcggtgtttg gcgagcaaaa gtgcttctcc aaacgagtga gttgcttcac
     1801 cacagacgcg gcagaccgga ttcacaggta tctccacagc tgacgtgcca gatagatgag
     1861 tcgatccact ggcctgacgc gaaagacacg atgctctgta aactcgtctg ccgcttcgtt
     1921 acgtgcaacg aatggtggat gacgaagagg ccagtccagt tctgtggttc cgagcgcctc
     1981 cagcggactc ttgcgttttg tatgtgtagc ttccgccccg aacccaatgt tacttacaag
     2041 gtttttgtct ggaagaatgg taagcccctg attcaagaga caggcgaagg tccactgaat
     2101 gtcccacgta ttgaagcctt tgcaaattag atcaaaattc ctggcccagt accttccagc
     2161 agggcctttg acaagttccc gaaatgaatg attgcgcgaa agctcgggcc aggaagcaag
     2221 atcaacatcg tatttctgcc aggctcttct ccaggtggcc caaccccaca catgagggta
     2281 gcgcgaaaaa tagtaggaat agccccgaga ctctggctgg aagttgtccc cggaaattga
     2341 aaacactctt tcatctgtgc gataacgcgc aagaagctca gaacaaaatc gaaagaaatc
     2401 gggatgtggc aggcaatcat cttccagaat gatcacttct tctgaccgct ccagggccca
     2461 gctgatgcca gaggacaatc gcgcgcgaca tcccatgttc tctgtagcaa agttggtttg
     2521 cacgtcgcaa gaccagttta catttgtaat aatacttctg acttcttcgc agagcttgct
     2581 ttctgtggcg ttccgggctc catccgcaat gagaaagaga cgcgccggtt tttgagcagc
     2641 aattctctca aacactcgtc gcgtaacgtc cggtcgattg aatgcaaaaa atactacggg
     2701 agtctctaga ctcatgcgat ctctccttcg cgacgaaacc cgaagaactc aaccaggggc
     2761 aaggcccaca ctttgagaag gcctgtagga atcgcccaga aaggcgcggc cagaaataga
     2821 aatgtggtca tgtagatcgg attgtgagac gttttcctgg aaaacaccgt cttcacataa
     2881 ggccagaaac gcatggactt tagcagatga tggccagtcg attctgtgtt catgcgaagc
     2941 gaaacggaag ggtcagaaaa aataggatgc cctttccttc ttgctcgcag agtgtattcg
     3001 acatcagaaa ggtagtgcgg aagaagagtt ggataaaatc caccaatgga tagaaactcg
     3061 gaaactctca tgcatagacc acgagtagta caccagtccg gcgtctcccc atctcgaatc
     3121 ggacgaaagc gcaatcgaag ccagtccact ttcatccttt gctcttgcag aatatcccgg
     3181 ccatctcttg cagtggcgac gaggagatcc cgcggccttt ctttcaatag gcgttctgca
     3241 ttttcaagaa acgcaggatc gagggccgta tcatcattca cggtcataat tacgtcagaa
     3301 gtaggaagct cagcgctccg ggcccagagg tacccctgat gcagactgcc tccccaccac
     3361 cagtttcctg taccgcgaat cacagtagtg aaaggaaaag attcgcacac catctctgac
     3421 gtaccgtccg tagatccatc gtctaccaga acgatgtgaa aatcttttga agaactttga
     3481 acaaggcttt ccacaaacgc gcgcgtaagt tcgatgcgat tgtgaacggg cagtattatg
     3541 tggaccatga ttctaaaact ggctctactt tgtgatctct gcgtaagtat tcaagccgat
     3601 ttgttctact cgcgaatcca gtcctttcag cggttcatgc cgcgactcgc cgtatttgat
     3661 ctcaaaatcg acactggaaa aaccaatgtg acggcacatg agttccagtt ccgctcgcga
     3721 agcaaagtgt ttgtgttcaa atacgtcata ggaatcatac acaccctgcc cgtaagaagc
     3781 cagacgattg tctgagaagt gacgcatcag tacaccctcc aaacctggaa aagacatgcg
     3841 aaggacgcct ccgggtgcaa gaacgcgata tgcttcgacg agaaagagaa gctgctgttt
     3901 ttgctccaaa tgctcaaaga aatcctcgga aaatgcatat aaaaaccaac catctgcaaa
     3961 aggatgttcc agagtcaaat tcgcggacac tctcaccgga tctgcaataa gagattccga
     4021 gggatagaga tctacattca cccagccgga catgagtctt ccgccggacc cgtagtggat
     4081 tttcctgctt tcgactttca ggattcgttt ttccgcactc actttccagc gaggcttacg
     4141 cgagatcgtg tatcccg
//
LOCUS       DAOQJL010000341         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13051_length_4154_cov_4.591120, whole genome shotgun sequence.
ACCESSION   DAOQJL010000341 DAOQJL010000000
VERSION     DAOQJL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13051_length_4154_cov_4.591120"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1662
                     /locus_tag="PLG78_13745"
     CDS             91..1662
                     /locus_tag="PLG78_13745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24231.1"
                     /translation="MKTVAAPIDTRVRDAYVKILLRIEEENGEKIINYARIALTALYF
                     ALGVLLGKEVAMSSRLTIFAGASASLIFSALTHYLIHYGRLTHKVKIFASVFDVAIVS
                     IALHQFGEYRTFKSEAYLLYFVWIALVNLRFTPVLTILASTSAIFFYALLALIAVQTG
                     TIQTGSLYDSYTSGSVSLTSVALKLLFLSIFAVAAVQAARGNRRLINKSVETELEAAR
                     QHEDRMKAQFASLAQTKQMLDSFSRFVPARFLEFLHVKDMVDIELGACVEKEITLLFV
                     DIRNFTGMSEKMSVFDNFRFLNAYLKRMGPIIEQQGGIIDKFVGDEIMCLFPDSPQKA
                     LVSSIEMRRELAIYNTDRKKMNYDALDIGAGLHTGNVMLGTVGSENRISTTVIGDNVN
                     LASRLQTLTKIFGVPIILSDAVYRRLDDPSAFCLREIDSVLVRGKEKPIVLYECFDMD
                     QDELRDQKKELHGDLQLALFHYKAGNFAESKQLFVDYEKKCVRDSIPRIYLKRLEKLM
                     QRPTSAKWSGVSRMK"
     gene            complement(1674..2684)
                     /locus_tag="PLG78_13750"
     CDS             complement(1674..2684)
                     /locus_tag="PLG78_13750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNE24232.1"
                     /translation="MTTMKAAAMQSFGGPEKINISDLPVPAIGPGEMLVRMKSASINP
                     IDWKTMLGKVKILIQYPFPLVPGSDGAGVVETIGPGVSRFKVGDRVYFRPGKDRIGTF
                     SQYFVVRETEAAVFPSKLSFDEAASLPLAGLTSWQALDLANVKAGDQVLIHAGAGGVG
                     ALAVQLARARGAIVTATCSAKNADFVRSLGATNIIDYRTEDYTSGGKRFDFVFDTQGG
                     KETNRCMDVLKPGGMVVSIANPPDPIEAVKFGRPWYVKLLLQLANIAPNRAARKRGVQ
                     YRALLMDASSSQLEEMAKLIEKGSLKPVIDKIFPFAETRDAIAYQMLGHSRGKVIIHI
                     ED"
     gene            complement(2771..3358)
                     /locus_tag="PLG78_13755"
     CDS             complement(2771..3358)
                     /locus_tag="PLG78_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNE24233.1"
                     /translation="MTKLKTAVVLTTALSAAALFAGDVQLDKSHTKVEFNVTHLTISD
                     VTGRFDEFEGKGSFDEKTGVLSALDVKIKVKSINTNDEKRDDHLRSADFFDASKYSEI
                     TFVLTKPVTLVKGGTANLQGNLTMRGVTKPITLQMNYKGTVKDPWGNVKAGFILTGKV
                     NRKDFGVSWSKNMDNGGAVVGDIVTINIAGEAAVK"
     gene            complement(3449..>4154)
                     /locus_tag="PLG78_13760"
     CDS             complement(3449..>4154)
                     /locus_tag="PLG78_13760"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNE24234.1"
                     /translation="SSDAVERDLTFGSLKLKHPEVIAVVERILPSDRFDELAEQDLRI
                     LELRFDSFSEPWSDVVAFAQSMRSRFAILGTCRENEENRNLLKQRYADFVPVVDCVDI
                     EIGTERALRSELIRITKDSSRQLMLSHHDFEKTPPDAELDRTIDDALLLRADFVKLAL
                     AAPSRQDAARLMFLLERRRDAHLSAFSMGSAGAVTRIAGGLFGSIFTYGYMDHPNAPG
                     QLSARELLALRKKFYS"
BASE COUNT         1047 a         1086 c          982 g         1039 t
ORIGIN      
        1 gtttgttcta cctccatggg ttcaatcgcc acatcgatgg ttcaagaaca aggacttttg
       61 ccctccaaac cgggacccgc cggaccgaca atgaaaacag tggctgcgcc aatcgatacc
      121 cgcgtccggg acgcatatgt caaaattctc ctccgcattg aggaggagaa cggcgaaaaa
      181 atcatcaact acgcgcgcat cgccctgacg gcactttact tcgctctggg cgttctactt
      241 ggcaaggaag tggccatgag ttcccgcctg accatatttg cgggagcaag tgcgtctcta
      301 atcttctccg cactcactca ttatctgatt cactacgggc gactcactca taaagtaaaa
      361 atcttcgcaa gtgtctttga tgttgcgatc gtaagcatcg cattgcacca gttcggcgaa
      421 tatcgcactt tcaaatcaga ggcctatctt ttatactttg tctggatcgc tctcgtaaat
      481 ttgcgtttta ctcctgtgct cacaatcctt gcgagcactt ctgcgatttt cttctacgcc
      541 ttactcgctc tcattgcggt acagacagga accattcaaa caggcagtct ctacgattct
      601 tacacaagtg gatccgtaag cctgacaagc gttgccctca agcttctatt tctctccatt
      661 ttcgcggtgg ccgcagtaca ggcagcgcgc gggaacaggc gcctcatcaa caaaagcgta
      721 gaaacagaac tcgaagctgc aagacagcat gaggatagaa tgaaagcgca gtttgcatct
      781 ctcgctcaga ccaaacagat gctggactct ttctctcgtt ttgttcctgc gcgtttcctc
      841 gaatttcttc acgtcaaaga catggtggac attgagcttg gagcatgcgt ggaaaaggaa
      901 atcactctgc tgttcgtaga tattcgcaac ttcacgggca tgtcagagaa gatgagtgta
      961 tttgacaatt tccgatttct gaacgcttac ctgaaacgaa tgggcccgat cattgaacaa
     1021 cagggcggca tcatagacaa gtttgttggc gacgaaatca tgtgtctgtt tcccgattcg
     1081 ccgcaaaagg ctttggtctc ctccattgaa atgagacgcg aattggccat ctacaataca
     1141 gatcgaaaga aaatgaacta cgacgcactg gacattggcg caggacttca cacagggaat
     1201 gtgatgctgg gcacagttgg atctgaaaac agaatcagca cgaccgtaat tggagacaac
     1261 gtcaacctcg caagccggct acaaaccctg actaaaatat ttggagtgcc catcattctg
     1321 tcggatgccg tctacaggcg gttagacgat ccgtccgctt tctgcttgcg cgagatcgat
     1381 tcagtactgg tgcgcggaaa agaaaaacca atcgtcttat acgagtgttt tgatatggac
     1441 caggatgaac tcagggacca gaagaaagaa cttcacgggg acctgcagct cgcacttttt
     1501 cattacaaag caggcaattt cgcagagtca aaacagctat tcgtcgatta cgaaaaaaaa
     1561 tgcgtgagag actccattcc acgcatctac ttgaagcgac ttgaaaagct catgcaacgc
     1621 ccgacttcag caaaatggtc gggcgtcagt agaatgaaat aatcctggaa gaatcaatct
     1681 tctatatgaa tgatcacttt tccccgcgag tgccccagca tctggtatgc gatggcgtct
     1741 ctggtctcgg caaaaggaaa tattttgtct atcactggct tcagggatcc tttctcgatt
     1801 aactttgcca tttcttcgag ctgtgaactg ctcgcgtcca tgagtaaagc gcgatactga
     1861 acgccgcgtt tccgcgcagc gcgattgggc gcgatgtttg ccagttgaag aagcaacttc
     1921 acataccagg gtctgccaaa cttcacagct tcaatgggat caggtggatt tgcaatgctc
     1981 accaccatgc cgccaggttt caaaacatcc atgcaacgat tcgtttcttt gccaccctgc
     2041 gtatcgaaaa caaaatcgaa tcgtttgccg cctgacgtgt agtcttccgt gcgatagtca
     2101 atgatgttgg tcgcaccaag agatcgaaca aagtccgcgt tcttcgcact acatgtagca
     2161 gtcacaatgg ctccacgggc ccgcgcaagc tgaactgcca gtgcaccaac cccacctgca
     2221 cctgcgtgga tcaaaacttg atctccagct ttcacattcg caagatcgag cgcctgccag
     2281 gacgtcaggc ctgcgagtgg aagagaagct gcttcatcaa agctcagctt cgaaggaaaa
     2341 acggcagctt ccgtttcgcg caccacgaag tattgagaga atgttccgat tctatctttg
     2401 ccaggtctaa aataaactct gtctcccacc ttgaatctgg aaaccccggg tccaatcgtt
     2461 tcaacgactc cggcgccgtc acttcctgga accagaggaa atggatattg tataagaatc
     2521 tttactttcc cgagcattgt tttccagtcg atcgggttga tgctggccga cttcatgcgc
     2581 acgagcattt caccgggacc gatggccgga acaggtaaat cagatatgtt tattttttca
     2641 ggtccgccaa acgactgcat ggcggctgct ttcatagttg tcataggcca caggattcga
     2701 gttacttcga accctgcgag acaaaaaaaa gggcggcacg agccgccctt acgatcacgg
     2761 ccgtctgcga ttatttaacc gcagcttctc cggcgatgtt gatcgttacg atgtcgccga
     2821 caacggcgcc gccattgtcc atgttcttgc tccagctaac gccgaagtct ttgcggttca
     2881 cttttccagt gaggatgaaa ccagctttca cattgcccca gggatctttg acggttcctt
     2941 tgtagttcat ctgcaaagtg atcggtttgg ttacgccgcg catggtaaga ttgccttgaa
     3001 gattcgcagt tccgcctttg accagagtca caggctttgt aagaacaaat gtgatctctg
     3061 agtatttgga cgcgtcaaag aagtcagcgc ttctcaggtg atcgtctctc ttttcatcgt
     3121 ttgtgttgat ggatttcact ttgattttca cgtcgagtgc ggaaagaact cccgtcttct
     3181 cgtcgaaact tcccttgcct tcgaattcgt caaagcgccc ggtcacatct gaaatcgtca
     3241 gatgcgttac attgaactca acctttgtgt gagatttatc cagttgcaca tctcccgcga
     3301 aaagtgcggc tgcggaaagt gctgtggtaa gaacgactgc tgtcttaagt ttggtcatgg
     3361 gaccctccat tgaatgcttg tgaatccata cggacttcaa tagtttcata ttaaagcatt
     3421 tggcgccagg cgaccaccga tttccaggtc aggaatagaa tttttttctg agggccagta
     3481 gctcccgggc agagagctgc cccggcgcat ttggatgatc catgtacccg taggtaaaaa
     3541 tagatccaaa aagaccaccg gcgatacgcg tgaccgcacc cgcgctgccc atggaaaatg
     3601 cgctgagatg cgcgtcccgg cggcgctcca gtaaaaacat gagtcttgct gcgtcctgtc
     3661 tcgatggcgc ggccagggcg agtttcacaa aatctgcgcg aagaaggaga gcgtcatcta
     3721 tggttcgatc gagctctgca tccggcggag tcttctcaaa atcatgatgg gacagcatga
     3781 gttgcctgga tgaatctttt gtgattcgta tgagctcgga tcgcagcgcc ctttccgttc
     3841 caatctctat atccacgcaa tctacaacag gaacaaagtc cgcataacgt tgcttgagga
     3901 gattgcgatt ctcctcgttc tcccggcatg tacccagaat cgcaaaacga gaacgcatag
     3961 actgtgcaaa cgcaaccaca tcactccagg gctcagaaaa agaatcgaat ctcaactcca
     4021 gaatccggag atcctgctct gccagttcat caaacctgtc cgatgggagg atgcgctcca
     4081 ccactgcaat cacttcagga tgtttgagtt tgagacttcc gaatgtcaga tcacgttcca
     4141 cagcatcaga agag
//
LOCUS       DAOQJL010000342         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13053_length_4154_cov_3.903391, whole genome shotgun sequence.
ACCESSION   DAOQJL010000342 DAOQJL010000000
VERSION     DAOQJL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13053_length_4154_cov_3.903391"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLG78_13765"
     CDS             complement(<1..305)
                     /locus_tag="PLG78_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HNE24235.1"
                     /translation="MRITDLMKNNTLVSNVNRHQYEMDKVQNQLATGQRIRQPSDDPG
                     AATNQMFFRTRVEELDQFQANIEDGHSRLMLMDGELDRVGAIFQRVRVLAVQASNGI"
     gene            complement(310..2244)
                     /gene="flgK"
                     /locus_tag="PLG78_13770"
     CDS             complement(310..2244)
                     /gene="flgK"
                     /locus_tag="PLG78_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNE24236.1"
                     /translation="MGSTFGGLEIGRKGLVAHQTALNTTGHNISNADNNGYSRQRVTM
                     EAADPLYEPSLNRASGPGQIGQGGSVSRIERVRDAFYDDQIIDATNRQNYWDASTNYL
                     GQMETIFAEPSDNTLRSLTEKFWASWQDLANYPSDAAHRSVVAERGNGLITRIHDIHD
                     KLGQLRIRANNEIVADVDILNSLAGEVRDLNEKILKLEALGDEPNDLKDKRDAVIEKM
                     SGIANIKVGRGDRDEVFVFIGEQTMVQGSILRKIKAEVDPANEGYAKVVWEHNSRDVI
                     LGGGHLSGLLEMRDHAIVERMDQNSLFAVNLADIVNEIHRDGFGINGTTNKDFFQIRN
                     LSPGSDGNYQLQNARANVDLNGDGTAELTAIFRTTGANSVDPSKKIGVDGTITFFKND
                     AQNTPVRIDYRRDQTLNEIIKKINDADGGVVAYMNHDNQLSIKATLASDDRRTNFMIR
                     HLEDSGELLVGYAGVLASSGEAGAFDFRRVNEIQKLRAPLQDIAFTPSFYPAGQISLS
                     SDIVRDPSNIAAARGRDLGGTGDYNTPNGAADGSNALLIAAGLKQDKKMFGHEETVDN
                     FYNALIAKLGTQARTAEDAAMRQKENLTQLGNFRQSVMGVNMDEEMSNMVQFQQSYQA
                     SARVINTMNEMVETLLKLGV"
     gene            complement(2337..3464)
                     /locus_tag="PLG78_13775"
     CDS             complement(2337..3464)
                     /locus_tag="PLG78_13775"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24237.1"
                     /translation="MRWQYCARCSALCIAVFSTVCSFPAGKTSGGLQSLLLADALFLS
                     GRLPRFLFATTNTGNVISRYRVEPLSGYLTFLGTTPSGGTSPRGASIDPTGRYLMVVN
                     NGSTNVSSYSINRFDGQLTLVGNTASGISPISAYFLPSGSFAYATDAGGAPYVMGYTM
                     NSSTGVLTNFAVGATGLNVGRGAVSDSASRFLYVGDFSGNFVRSFTINANGTLTSLNS
                     VATGVNPHSLAMHPGGQYLYASLNSGSLVAFRVASDGTISMTATVAVSGTPQTIAIDP
                     LGRYVYLVNATGGVLYVFSIADPTSPVLLYTFVMGPQANAVRVDPSGRFVYILCGTGS
                     NQIFALYLDPFWGFSFLTALSTGSNAGAMEGIAIASYSISE"
     gene            complement(3495..>4154)
                     /locus_tag="PLG78_13780"
     CDS             complement(3495..>4154)
                     /locus_tag="PLG78_13780"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24238.1"
                     /translation="TLETTEKDPDALLPAALFNLQEFHDLFSAESLAADLQLEIGEQT
                     ILFTDMVGSTSFYASSGDSKAFVAVKKHFEEVYREVRDANGAVIKTIGDAVMASFADP
                     VDAMHAAVSLQNKFGPSSPSPIRLRVSIHTGQCIAVNLNSGIDYFGTTVNVAAKIQNV
                     AGAGEIACTAQFLAKPGVRAFLSQRGLSGESMPYRIPGLSDTWSLERFRITAPEVARN
                     A"
BASE COUNT         1007 a         1089 c         1038 g         1020 t
ORIGIN      
        1 tagatcccgt tggaagcctg caccgcgagc actcgaacac gctgaaaaat cgctcccaca
       61 cgatcgagtt cgccgtccat gagcatcaga cgagaatgac cgtcctcgat gttggcctgg
      121 aactgatcga gctcttccac ccgagtgcga aagaacatct gattggtggc ggctccagga
      181 tcatcgcttg gttgccgaat gcgctggccc gtggccagtt gattctggac tttgtccatt
      241 tcatattgat gccggttcac attggaaacc agcgtattgt ttttcatcag gtccgttatt
      301 cgcatcatat tacacgccca gcttcagtag agtttctacc atttcgttca tagtgttgat
      361 cacgcgcgca gaagcctggt agctctgctg gaattgcacc atgtttgaca tttcttcatc
      421 catgttcaca cccatcacgc tctgtctgaa attgcccagt tgagtgaggt tctctttctg
      481 acgcatggct gcgtcttccg cggtgcgagc ctgtgttccg agcttcgcaa tcaacgcatt
      541 gtaaaaatta tcaactgtct cctcatgccc gaacatcttc ttgtcctgct tgagacccgc
      601 ggcaatcaac agcgcattgg aaccgtctgc ggctccattc ggagtattgt aatcccctgt
      661 tccaccaagg tcacgtccgc gagcggcagc aatgttggat ggatcacgaa caatgtcaga
      721 agacagagaa atctgtcccg caggatagaa acttggagta aacgcaatgt cctgaagcgg
      781 cgcacgcagt ttctggatct cattgactct tctaaaatca aatgcacccg cttcccctga
      841 agacgcgagc actccggcgt atccaacgag aagttctccg gaatcctcga ggtggcgaat
      901 catgaaattg gttcttctat catcgcttgc gagagttgcc ttgatggaaa gctgattgtc
      961 gtggttcatg tatgccacaa caccaccgtc cgcatcgttt attttcttta tgatctcatt
     1021 gagagtctga tcacgacgat agtcgatgcg aaccggagtg ttctgcgcat cattcttgaa
     1081 gaatgtgatg gttccatcca cgccgatctt cttgcttgga tccacggagt tcgcgcctgt
     1141 tgtgcgaaag atcgcagtga gttcagccgt tccatctcca ttgagatcca cattcgcgcg
     1201 tgcattctgg agctggtagt ttccgtctga tccggggctc aggttcctga tctgaaagaa
     1261 atctttgttt gtggttccgt tgattccaaa accatctctg tggatttcgt tgacgatatc
     1321 cgccagattg actgcaaaaa gactgttctg atccatgcgc tctacaattg catggtcgcg
     1381 catttcaaga aggccggaaa ggtgtccgcc gccaagaatc acatctctgc tattgtgctc
     1441 ccaaacaacc ttcgcataac cttcgttcgc tggatctact tccgctttga ttttgcgcag
     1501 gatggagccc tgaaccattg tctgttcgcc aatgaatacg aatacctcgt cacggtctcc
     1561 tcgccctact ttaatgttgg caattccgga catcttctct atgactgcgt cgcgcttgtc
     1621 tttcagatcg tttggctcat ctcccagggc ttcgagcttc agaatttttt cattgaggtc
     1681 acgcacctca ccggcaagcg aattgaggat gtccacgtcc gctacgatct cgttgtttgc
     1741 gcgaatgcga agttgcccca gtttgtcatg aatgtcgtga atgcgagtga tgaggccatt
     1801 tcctctttct gcgaccacag acctgtgagc tgcgtcggac ggatagtttg caagatcctg
     1861 ccagctggcc cagaatttct cagtcaagga gcgaagagtg ttgtcggatg gctcagcaaa
     1921 gatcgtctcc atttgtccca ggtagttggt agaagcatcc cagtaattct gacgattggt
     1981 tgcgtctatg atttgatcgt cgtagaatgc atcgcgcact ctttcaatgc gcgaaacact
     2041 tccgccctgg ccaatttgcc cgggaccgga agcacgattc aaagaaggct catacagtgg
     2101 atctgccgct tccatggtga cgcgctggcg ggaataaccg ttgttgtcgg cattcgatat
     2161 gttgtggcca gtcgtgttca gggcagtctg gtgcgcaacc aggcccttcc ttccgatttc
     2221 gaggccgccg aatgtagaac ccatatgtcc tcccgccgtg catggcacga gcgattcgtg
     2281 tctatcgaac gtgtcggaca ttcagcgcaa aagatgatta aaaaaccctg tgaattctac
     2341 tctgaaattg agtaagaggc aatcgcaatg ccttccattg cgcctgcatt gctcccggta
     2401 ctgagcgcgg taaggaagct gaaaccccag aatggatcca gatacaaagc aaaaatctga
     2461 tttgaaccgg tgccgcacag aatgtagacg aatcttcccg acgggtcgac cctgaccgcg
     2521 ttggcctgcg ggcccataac gaatgtgtat aataaaacag gcgatgtggg atctgcgatg
     2581 gagaatacat aaagcacacc gcctgtcgcg tttacaaggt agacgtatct tccgagtgga
     2641 tcgatggcaa ttgtctgcgg ggttccggaa acagctacgg tagcggtcat agatattgta
     2701 ccatcgctgg ccactcgaaa cgcaaccaga gaaccagaat tcaaggacgc atacaggtac
     2761 tggccgcccg gatgcattgc cagactgtga ggatttacac ctgtcgccac actgttcaaa
     2821 gatgtcagag ttccattcgc attgattgtg aacgatcgaa caaaattgcc agaaaaatcg
     2881 cccacgtaca ggaagcgact ggcagaatca gaaaccgcgc cacgaccgac gttgagaccg
     2941 gttgctccga ctgcaaaatt agtcaggaca ccggtggaag aattcattgt gtaacccatc
     3001 acgtaggggg caccgccggc atctgtcgca taagcaaaag agcccgacgg aagaaagtag
     3061 gcactgattg gactgatccc ggatgcggta ttgccgacta acgtcagttg accatcaaaa
     3121 cgattgatgg aataggatga tacattggtg gatccattgt ttacaaccat taagtatctt
     3181 cctgtcggat caatggacgc gcccctgggt gaagtgcctc cggaaggggt tgttcccaga
     3241 aatgtaaggt aaccggagag cggctccact ctatatcttg agatcacgtt cccggtgtta
     3301 gtcgtcgcga acagaaagcg cggcaatctt ccggacagga agagcgcatc cgcaagcaac
     3361 aacgattgga gccctccaga tgtctttcca gccggaaatg agcaaacagt agagaaaacc
     3421 gcgatacaaa gggcactgca acgcgcacag tattgccagc gcataccctt cgatagcgct
     3481 gttctcaatt catgtcaagc gttccgcgca acttctggag cagttatgcg gaatcgctcc
     3541 agagaccacg tatccgaaag acctggaatg cggtagggca tagattcgcc ggacaggcca
     3601 cgctgggaaa ggaaagcgcg cactcctggc tttgcgagaa actgggcagt acatgcgatt
     3661 tccccggcac cggctacatt ctggattttg gccgctacat tcacagtggt tccaaaatag
     3721 tctatgccgc tgttcaaatt cacagcgatg cactggccag tgtgaatcga aacgcgaagc
     3781 cgaattggag atggcgacga aggaccaaat ttattttgaa gagaaacagc cgcatgcatt
     3841 gcgtcgaccg ggtctgcgaa agaagccatc acagcgtcgc ctatcgtctt gatcacagca
     3901 ccgtttgcgt ctctcacttc cctgtagact tcttcaaaat gcttcttcac cgcaacaaac
     3961 gcttttgaat ctccactgga agcgtaaaag ctggtagaac caaccatgtc cgtaaacaga
     4021 atcgtctgct ctcctatctc aagctgcagg tccgcagcca acgattctgc ggaaaacaga
     4081 tcgtgaaact cctgtaagtt gaaaagggct gcaggcaaaa gagcatccgg atctttttcc
     4141 gtagtttcca gagt
//
LOCUS       DAOQJL010000343         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13079_length_4149_cov_3.845628, whole genome shotgun sequence.
ACCESSION   DAOQJL010000343 DAOQJL010000000
VERSION     DAOQJL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13079_length_4149_cov_3.845628"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1830)
                     /gene="ilvB"
                     /locus_tag="PLG78_13785"
     CDS             complement(145..1830)
                     /gene="ilvB"
                     /locus_tag="PLG78_13785"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNE24239.1"
                     /translation="MKQIRRQSLGKGDCVKSGARIILDYLEQESVPFVAGMPGGANLP
                     LYDALHSSSIVSILARHEQGAGFIAQGYARATGKVGVCFATSGPGATNLITSLADAKL
                     DSIPIIAITGQVPRDLIGTDAFQEVDTIGIARPGVKEAFFARDAQSLYDILPAAFAAA
                     RSGRPGPVLIDVPKDVQREVAAGRYAIMEPSRGTLSSASVEEAARMIQASRRPVLYAG
                     GGIIHSNASEILIQIAERNAIPVVTTLMGIGSIPTSHPRFFGMLGMHGEPYTNLLLEE
                     CDLLIAVGSRFDDRATGKLSEFCPDAKVIHVDIDRTELGKLRKPDLAIQADARTFLSA
                     LRNILPFHERREWTALCDDLRRDHPSENHDGNSEALSYLREISDSISDHDVITTDVGQ
                     HQMWAAQVCRIREPRTFLTSGGLGTMGFGLPAAIGAALGTGKRAVCISGDGSILLNVQ
                     ELALLADLNLDVKVVVFDNANLGMVRQQQQMFYGQRYSSSRFERAVDFVSLARSFGIP
                     GYSASDRPISSILREPGPALIHLPIDENENVYPMVAPGSANRDMIRRPAGKNS"
     gene            complement(1992..3260)
                     /locus_tag="PLG78_13790"
     CDS             complement(1992..3260)
                     /locus_tag="PLG78_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE24240.1"
                     /translation="MKFVKAEEAVSVIQSGNRIFVHGVAATPHRLIDGMMARASELRD
                     VELVHIHTEGGAPYAKPEFASSFKTNVFFVGGNIREAVQNHRADYVPVFLSEIPALFR
                     KKILPIDVAMIHVSPPDNHGYCSLGVSVDIARAAVQCAGKIIAQVNPAMPRTHGESMV
                     HVSQIHALIDANDALPEVAPTEPDEIELAIGKHVAGLVEDGATLQMGIGAIPNAVLSC
                     LTGHKKLGIHTEMFSDGVIPLVEKGVITGEMKVKHPGKIVSGFVMGTRKLYDFIDDNP
                     FVSMLDIAYVNDTAVIRRNPKVTAINSAIEVDLTGQICADSIGHRQFSGVGGQMDFVR
                     GASLSEGGKPIIALPSQTSKGESRLVSCLKPGAGVVTTRAHVHYVITEFGVADLYGKN
                     LRQRARALIGIAHPSHRDRLTEEARKVLGI"
     gene            complement(3268..>4149)
                     /locus_tag="PLG78_13795"
     CDS             complement(3268..>4149)
                     /locus_tag="PLG78_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE24241.1"
                     /translation="HQDAIRRMRAEKIELSIFRPLGWFGMLSIRNHRRSHRKLVVIDG
                     NICFAGGMNIVRDHSRRAVGHESWRDSMIRLVGRIAMDAELSFDRMWRFVSGRRLVFP
                     RSRPAGVVYSSADLQLIENTPRFRLAFRRAYRDSLASAQKRIWIESAYFIPTLSFLRL
                     LKRKARAGVDVRLLVSEKSDVKAAFYASRFVFSGLLKAGVRIYEVRARFVHSKALVVD
                     DRISIVGSANLDHRSFLHDLELSIRSTRPDINHDLAHQFSVDASRARLIDGVEWGKRP
                     FTVKLLERWFFLFKFYL"
BASE COUNT         1046 a         1142 c          988 g          973 t
ORIGIN      
        1 ctttttcctc tgaaagcctg ctgatctcag cgttctttgc tttcatgtca ttttcataag
       61 tagatgaaga aacgcacgat accaccagaa gcggcgcaag tagaaacagt cgcatgccct
      121 gatatgtccc gaggaacgag gctttcaact gtttttcccc gcaggtcgcc tgatcatatc
      181 tcgattcgcg cttcccggcg ccaccattgg atacacgttc tcattttcat caatgggaag
      241 atgaatcaac gcaggccctg gttctctcaa aatgctggag atgggccggt cactggcaga
      301 atatcctgga attccgaaac ttctcgcaag agatacgaaa tcaaccgcac gttcgaacct
      361 ggaagatgag tacctctggc cgtagaacat ctgctgttgc tgacgcacca tccccaggtt
      421 cgcgttgtca aaaactacaa ccttcacgtc cagattcaaa tccgcaagaa gagcaagctc
      481 ctgtacgtta agtaaaatgg aaccatcacc ggaaatacac actgctctct ttcctgttcc
      541 aagtgcagca cctatcgcag ccggaagacc aaagcccatg gttccaaggc cgcctgacgt
      601 taaaaatgtg cgcggctctc tgattctgca tacctgagca gcccacatct ggtgctgacc
      661 cacgtcggta gtgatcacat catggtcgga aatcgaatca gaaatctcgc gcaagtagga
      721 aagagcttct gaatttccgt catggttttc agatggatgg tcgcggcgaa ggtcatcgca
      781 cagtgcagtc cattctctgc gctcatggaa aggcagaatg tttcgaaggg cagacaaaaa
      841 cgttctggca tccgcctgaa tggccagatc cggctttctg agtttgccca gttcagttct
      901 gtcaatgtcc acgtgaatca ccttcgcgtc cggacaaaac tcggaaagct ttcctgtggc
      961 tcgatcatcg aatctagaac caaccgcaat caacaagtcg cattcttcca aaaggagatt
     1021 ggtgtatggc tctccgtgca ttccgagcat tccgaaaaat ctgggatggg atgtgggaat
     1081 cgatccaatg cccatgagag tcgtcacaac tggaatcgca tttctttctg cgatttgaat
     1141 caggatttca gatgcattcg aatgaatgat gcctccaccg gcgtaaagta cgggccgtcg
     1201 cgaagcctgg atcatcctgg ccgcttcctc cacggaagcg ctggaaagtg ttcctcgcga
     1261 tggttccatg atcgcataac gtccggccgc cacttccctc tgaacgtcct tgggaacatc
     1321 aatcagaacg ggtcctggcc tcccgcttcg cgcagctgca aaagccgcag gaagaatgtc
     1381 atagagagac tgtgcatcgc gcgcaaagaa cgcttccttt acccctggtc ttgcaatccc
     1441 gatcgtatcc acttcttgaa atgcatctgt tccgattaaa tctcgcggca cctgacctgt
     1501 gatggcaatg atgggaatcg aatctagctt cgcatctgcg agggaagtga tcagattggt
     1561 ggcacccggg ccggaagtag caaagcacac acccactttg cctgtggccc tggcatagcc
     1621 ctgcgcaatg aaccccgctc cctgctcgtg ccttgccaga attgacacga tggagctaga
     1681 atgcagcgca tcgtagagtg gaagattggc tcctccaggc attccggcta caaaaggcac
     1741 ggattcttgt tccagatagt cgaggatgat tcttgctccg ctcttcacac agtctccttt
     1801 tccgagggac tgccggcgga tttgtttcat cccgccggct gcgccgacgg gatcgtgtcc
     1861 ttacggcgca gaaatgaata ctacgaccac gaggaccagg accagtgtat tcattgcgcg
     1921 ggacatttga agccacgaaa actacaaagc caggaatcgt ctttcaaatt cctggctttg
     1981 atctatattt tttaaatgcc cagcacctta cgggcttctt ccgtcaggcg atcacggtgc
     2041 gatggatgag caattccgat gagagcgcgc gccctctggc gaaggttttt tccatataaa
     2101 tcagccacgc caaactccgt aatcacgtag tgcacatgcg cacgagtggt cacgacaccc
     2161 gcgccaggct tcagacaact gacgagcctg gattctcctt tggatgtctg gctcggaagt
     2221 gcgatgatcg gctttccgcc ttcagagaga gaagcgcctc gaacaaagtc catctggcct
     2281 cctactccag aaaactgacg atgaccaatc gaatcggcac aaatttgacc tgtgagatcc
     2341 acttcgatgg cactgttgat ggcagttact ttaggattac gtcgaatcac tgcagtgtca
     2401 ttgacatagg caatgtccag catggacaca aacggattgt catcgatgaa atcgtagagc
     2461 ttgcgagtgc ccattacaaa cccggaaacg attttccctg gatgcttcac tttcatttcc
     2521 cccgtaatca ctcctttctc gacgagaggg atgactccgt ctgaaaacat ttcagtatga
     2581 attcccagtt tcttgtgacc ggtcagacag gagagcactg cgtttggaat ggcaccgatt
     2641 cccatttgaa gagtggctcc gtcttccacg agacccgcca catgtttgcc aatggccagt
     2701 tcgatctcgt ctggttctgt aggggctact tcagggagcg catcattcgc atctatcaaa
     2761 gcatggatct ggctgacgtg gaccatgctt tcgccgtgag ttctgggcat ggcaggattc
     2821 acctgtgcga tgattttacc cgcgcactga accgcggcgc gtgcgatgtc tacggaaaca
     2881 cccagggaac agtatccatg attgtctgga ggagaaacat gaatcatggc cacgtcaatg
     2941 ggcaggatct tttttctgaa aagtgcgggg atctcgctga gaaagaccgg aacgtaatcc
     3001 gctctatgat tctgaaccgc ttctcgaata ttccccccga caaaaaagac attggttttg
     3061 aaactggacg caaactccgg cttcgcatag ggagcacctc cctctgtgtg tatgtgcacc
     3121 agttctacgt cgcgcagttc ggaggctctg gccatcattc catctatcaa gcgatgagga
     3181 gtggcggcga cgccatgtac aaaaatacga ttccccgatt gaatcacact gactgcttct
     3241 tctgctttta cgaatttcat aatcccctca caggtagaat ttgaatagaa agaaccatct
     3301 ctcaagcaac ttgacggtga aaggccgctt cccccactct actccatcaa tcaaccttgc
     3361 ccgggatgca tcgacagaaa actgatgagc aagatcatga tttatgtcag gccttgtgga
     3421 ccgaatgctc aattccagat catgcaaaaa gcttctgtga tccaggttcg cagatccaac
     3481 aatagaaatc cgatcatcca cgaccagggc tttagaatgc acaaatcggg cacggacttc
     3541 ataaatgcgc acgcccgctt tcagtagacc cgaaaaaaca aatctgctcg catagaaggc
     3601 ggcctttacg tcgctcttct cactcaccag caagcggaca tccactcccg cacgtgcctt
     3661 tctcttgaga aggcgcagga aagataaagt cggaatgaaa taggcagact caatccagat
     3721 tctcttttgc gcagacgcta gagaatcacg atacgctctt cgaaatgcca ggcgaaagcg
     3781 cggcgtgttc tcaatgagct gcaaatcggc tgacgaatat accactcccg caggccgcga
     3841 tctcggaaac acaagccttc tgcccgaaac aaaacgccac attcgatcaa aagaaagttc
     3901 tgcatccatg gcgattcggc ccacgagccg aatcatagaa tcccgccagc tttcgtgacc
     3961 cacggcacgc ctgctgtgat ctcgcacaat gttcataccg cccgcaaaac agatatttcc
     4021 atcgatcact acaagcttcc gatgactgcg gcgatgattg cgaatagaga gcattccgaa
     4081 ccatccgaga ggccgaaaga tgctcagttc aattttttca gcacgcatgc ggcgtatcgc
     4141 atcctggtg
//
LOCUS       DAOQJL010000344         4143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13108_length_4143_cov_4.424168, whole genome shotgun sequence.
ACCESSION   DAOQJL010000344 DAOQJL010000000
VERSION     DAOQJL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13108_length_4143_cov_4.424168"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLG78_13800"
     CDS             complement(<1..878)
                     /locus_tag="PLG78_13800"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNE24242.1"
                     /translation="MSIFRRLLRSLTWHWKEKIICLLMASGLWFYVDNLKIGTISLNV
                     PVSYRNKPVDLDFAQDPPRFIEVKLRGDKERLNFPTTKLRAEVDLKGATAERKTYAYS
                     IDTRLFPENVEMVDTVTSLHIQLEKSMVRFVVLKATTMGSPKSGFKRGRVLIMPDRAK
                     VQGGTERMQSVREIMLPPIDITGASSNVMRSVPVVEPPGTTLLSPPEVEVTVVIVSDE
                     KESEKTLEIPVTFRNASPGVTPTSQTRTVRVTVHGDSDMIKDVGESDVDAFLDLAGLD
                     LTGDVDMVTFEIPVRVV"
     gene            925..2226
                     /gene="murA"
                     /locus_tag="PLG78_13805"
     CDS             925..2226
                     /gene="murA"
                     /locus_tag="PLG78_13805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNE24243.1"
                     /translation="MQSPVFRVRGGVPLSGTIRPQGNKNEALPLIAASILTDEPITLS
                     NIPDIEDVRLMVDIVRSLGSSVQEDFQMGPESSIRIHTKEQPQSDLPVDLSTRLRGSV
                     TLAGPLLARTGRVFLPRPGGDRIGRRRLDTHILALEALGAKLKAFPDGFELTTDRLKG
                     ADILLDEASVTGTENAVCAAVLAEGDTVIRHAASEPHVQNLCHFLVSMGAKIEGIGSN
                     ILRIHGVSSLRGTSHRIGPDYLEVGSFISLAAVTRGELLIEDADLENLRMIRLVFSRL
                     GIETRPEGDALRVPGDQSMEIVSDLGGAVPHVDDAPWPGFPSDMTSVALVTATQCKGT
                     LLIHEKMFESRLFFTDRLISMGARIVLCDPHRAVIIGPSPLSGSRISSPDIRAGMAML
                     IAALCAQGESEIQNIIQIDRGFAHIDERLRSVGALIERAEG"
     gene            2230..3084
                     /locus_tag="PLG78_13810"
     CDS             2230..3084
                     /locus_tag="PLG78_13810"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE24244.1"
                     /translation="MRSLSARPLLICALFLNIALLATPEQDHSLRIMFWNVHNLFDTA
                     NDPRTRDDDFTPSGKEKWTDEKLRIKIKALASVIREVNPDVGGFAEVENIEVLRMLGT
                     EAGYPYAYLIERGDQRGIDTGVLSKTSLQELQNKGPGRGLIHFKIKGVTIAFAHWKSK
                     RGGKNQTEEKRLQAARYAVTVTDPLLLTGDFNEGPHEKAREVLEEAGFRNLVTGPCSS
                     FFSGKKGTSKSCIDGAYLRGECISGRAEIVHPHSLAQGSKPNPEISDHLPVVTILNFC
                     EKESESAR"
     gene            complement(3108..>4143)
                     /locus_tag="PLG78_13815"
     CDS             complement(3108..>4143)
                     /locus_tag="PLG78_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNE24245.1"
                     /translation="LVAAYNRSRDSGVTTEISAMASFGLGMLCRTHPPEAGILALLIT
                     ILLSSKRLTHSTITKMRRVELTDTLKFLVIIFIVLPLLPNHAVDPYGAFNPYKVGLMV
                     VLICGISFSGYFLTRILGTQRGLGLTGILGGMTSSTAVTAAMSTEARRNPNNALICAF
                     STVAANATMFIRVAVVVAFVDFSLLTRLVLPLGAMALVAILAAVILWMIAARRQSGVD
                     VVDRRQLELSNPFSIGPALKFAVFFVFVLFAAKFAQTMFGNSGLYAAAAVSGLADVDA
                     ITLSIAEQAGNGALERITGAMGIIIAVVSNGIVKTGIAFYSGDLRFGRLIGVCLGAAT
                     GTGLLLASFS"
BASE COUNT         1052 a         1098 c         1050 g          943 t
ORIGIN      
        1 actactcgga ccggaatctc aaaagtcacc atatccacgt ctccggtcaa atccagccct
       61 gccagatcca gaaaagcatc tacgtccgat tcaccaacat ccttgatcat gtcactgtcc
      121 ccatgaacgg tcactcgcac cgtcctggtt tgcgaggtgg gagttacacc gggggatgca
      181 ttgcgaaatg ttactggaat ctcaagagtc ttttcgcttt ctttttcatc cgacacaatc
      241 accacggtga cctccacctc cggaggcgaa agcagggttg ttccgggtgg ctccacgact
      301 ggcactgatc gcataacgtt agaggatgcg cctgtgatgt ctatcggggg caacataatc
      361 tctcgaacgg attgcattct ttctgtaccg ccctgcactt tcgcgcgatc aggcatgatc
      421 aaaactcgtc cgcgcttgaa tccgctcttc ggggatccca tggtcgtggc ttttaagact
      481 acgaagcgaa ccatggattt ctcaagttga atatgcaggg acgtgaccgt atcgaccatt
      541 tcaacgttct cggggaacag tcgcgtatca attgaatacg catatgtttt gcgctcggcg
      601 gtagcgccct tgaggtccac ttcagcgcgg agttttgttg tgggaaaatt gagcctctct
      661 ttatctccgc gcaacttcac ttcaatgaaa cgcggaggat cctgggcaaa gtccaggtcc
      721 acgggcttgt tgcgatagga gacaggcacg ttgagactaa tcgttccaat tttcaaatta
      781 tcaacgtaga accagaggcc cgaggccatc aacagacaga tgatcttttc tttccagtgc
      841 caggtcaggc tgcgcaatag acggcgaaag atcgacattc aggattcccc ttgacgtagg
      901 cctcgatgca aacaaggctt gcccgtgcaa tctcctgtat ttcgtgtccg tggaggagtt
      961 ccgctttcgg gcacaattcg cccgcagggg aacaaaaacg aagcgcttcc attgatcgca
     1021 gcttccatcc tgacggacga gccgatcacg ctatccaaca tacccgacat agaagacgtg
     1081 cgcctcatgg tcgacatcgt ccgatcgctg gggtccagcg tccaggaaga ttttcagatg
     1141 gggcccgaaa gctctattcg aattcacacg aaagaacaac ctcagagcga tttgcccgtg
     1201 gacctttcca caagactgcg gggttctgta acccttgccg gaccacttct cgcgagaacg
     1261 gggagagtat ttcttccaag gccaggggga gacagaatcg gcagaagaag actggacacg
     1321 cacatccttg ctctggaagc actgggcgca aaactaaaag cattcccgga cggatttgaa
     1381 ttaaccacag atcgtctcaa aggcgcggat attcttctag acgaagcgtc cgtcaccgga
     1441 acagaaaacg cagtgtgcgc ggctgttctt gcggaaggag ataccgttat tcgccatgct
     1501 gcctctgaac ctcatgttca gaatctatgc cacttcctgg tttccatggg agcaaaaatt
     1561 gaaggcattg gatccaacat tcttcgcata cacggcgttt catcgcttcg cggaacttcg
     1621 catcgcattg gacctgacta tctggaagtc ggaagtttca tatccctggc cgctgttaca
     1681 cggggtgagc ttctcattga agatgcagac ctggaaaacc ttcgtatgat ccgcctggtc
     1741 ttttcgcgac ttggaattga aactagacca gagggagacg cccttcgagt tccaggagat
     1801 cagtccatgg aaatcgtaag cgatctcggc ggagccgtac cgcatgtaga cgatgctccg
     1861 tggcctggtt ttccttctga tatgacgtca gtcgctcttg taactgcgac tcaatgcaaa
     1921 ggcacgcttc tgattcacga aaaaatgttc gaatccagac tcttctttac ggatcgtttg
     1981 atttccatgg gtgcacgcat cgtactgtgc gatccgcatc gcgctgtcat cattggacca
     2041 tcccctctgt ccggttcacg gatctcaagc cccgatattc gagctggtat ggccatgctc
     2101 atcgctgcac tctgcgcaca gggtgaatcg gaaatccaga acatcattca aatagaccgt
     2161 ggctttgctc acattgacga aaggttgcgc agtgtgggcg ctttgatcga acgcgcagag
     2221 ggttaacgag tgcgaagcct cagcgcacgc ccccttctaa tttgcgctct ctttcttaac
     2281 attgcattgc tggcaacgcc agaacaagat cactctcttc gcattatgtt ctggaacgtc
     2341 cataacctgt ttgatacagc caatgatcca agaacgcgcg atgacgattt taccccatcc
     2401 ggaaaagaaa agtggaccga tgaaaagctt cgtatcaaga tcaaggctct tgcatctgtc
     2461 atccgcgagg tgaacccgga tgtaggcgga tttgcggaag tagaaaacat cgaagtgcta
     2521 cgaatgcttg gaactgaggc gggctatccc tacgcgtatc tgatagagcg cggagaccag
     2581 agagggattg atactggggt tctttcaaaa acatcgcttc aggagttgca gaacaaaggg
     2641 cccggccgtg gtttgattca tttcaaaatc aaaggggtta ctattgcatt tgctcactgg
     2701 aaatcaaagc gcggtggcaa gaatcagacg gaagagaaac gtctgcaggc agcgcgatac
     2761 gcggtcacag tgacagaccc gctccttctg acaggcgatt ttaacgaagg cccgcacgaa
     2821 aaagcgcgag aggttcttga ggaagctggc tttcgcaatc tcgttaccgg tccttgctct
     2881 tctttttttt cgggtaaaaa agggacgagc aaaagctgca tagacggcgc ttatctgcgc
     2941 ggagagtgca tctcaggccg tgcagaaatt gttcacccgc actctctggc tcaagggtcg
     3001 aagcccaatc cggagatttc agatcatctc ccggtagtta cgatcctcaa cttttgcgaa
     3061 aaagaatctg agagtgcgcg ctagcgcaat tctacacttt gcgaatttca ggaaaaagag
     3121 gcgagtagca aacccgttcc agtcgccgcg cccagacaga ctccgatcag gcgaccaaac
     3181 ctgagatctc ctgaataaaa cgcgatgcca gtcttcacga tgccgtttga aacaacagca
     3241 atgatgatcc ccatagcacc cgttatgcgc tcaagtgctc cattcccggc ctgctccgcg
     3301 atactgagtg tgatcgcatc cacatccgcc agaccgctca cggctgccgc cgcgtacaga
     3361 ccggagttgc cgaacatggt ctgcgcgaat tttgccgcga aaagaacgaa tacaaaaaaa
     3421 accgcaaatt tcaaagcagg tccaatggag aatgggttgc tgagctctaa ttgccttctg
     3481 tcaacaacat cgaccccgct ctgacgacgt gcggcaatca tccagagaat cacggccgcg
     3541 agaatcgcca cgagagccat cgcacccaga ggcaagacaa ggcgagtcag caaagaaaaa
     3601 tctacgaaag ccaccacgac tgccactcgg ataaacatgg tagcatttgc tgcgacggtg
     3661 gaaaaggcac agatgagcgc gttgttcgga tttcgccgag cttccgtgct catggccgcg
     3721 gttacggctg tggaggaagt catgccgcca aggattccag tcaaacccaa tcctctctgc
     3781 gttccaagga ttcttgtaag aaaataccct gaaaaactaa tgccgcagat caacacaacc
     3841 atcaggccca ctttgtatgg attgaaagcg ccgtaaggat ccacggcgtg atttggaaga
     3901 agcggaagta caatgaatat gattacgaga aacttcaacg tgtcggtgag ttcgacgcgc
     3961 cgcatctttg taattgtaga atgagtcaat ctcttggagc tcaataagat cgtgatgagc
     4021 aacgctaaaa ttccggcctc cggaggatgc gttcgacaga gcatgcccag accaaaactg
     4081 gccatggcag agatttctgt agtgactccg ctatcgcggc ttctgttgta tgcagcgaca
     4141 agg
//
LOCUS       DAOQJL010000345         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13129_length_4137_cov_3.892945, whole genome shotgun sequence.
ACCESSION   DAOQJL010000345 DAOQJL010000000
VERSION     DAOQJL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13129_length_4137_cov_3.892945"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLG78_13820"
     CDS             complement(<1..420)
                     /locus_tag="PLG78_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNE24246.1"
                     /translation="MDLSTIAYHKSEFKKLSDCTVNVATHALQYGTAVFGGVRGYYSK
                     GMDNLLIFRIDRHFKRLHQSAKMMRMTPPLSEKEMANILIQVARFNNYRQNVYFRPFL
                     YKAAEQLSPRFHDVKDEFSLYSLRLDDYLETNKGLSAC"
     gene            complement(486..2201)
                     /locus_tag="PLG78_13825"
     CDS             complement(486..2201)
                     /locus_tag="PLG78_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE24247.1"
                     /translation="MLISRLSIRARIWMLALGFFATFLFAGVFVAVSEMALSSRVNDA
                     VARMELVRQSQVHFKLQVQEWKNILIRGSDPEAYKKHLIAFRTESTAFQALLQKLIES
                     ETNSDTKSKAIEIQKRHSDLLKQYEVTLEELSAGSPDFTRAADRRVKGMDRPTDQQLS
                     ALVELISNQSKGNQERDLRLFWISLGSAGLIVTVLTFLFSWMAVRSVSNTLGAVTGTL
                     QQISEGKLNLEIDDSSSDERGTILSAVKRSSRKIAAMLLSVRDQVHQTRGAAFEISGT
                     AKKLMKASQNLAASSEESAAAVEELSAISDHVSDSMTVVAQNSKEVDHSIHQLATESE
                     EMRMSMQTLGARNLESSNRARSGLQQIQIVSESMEQVKDGSQKIGAIMSLMHELSDRI
                     NLLALNASIEAARAGEAGRGFAVVAEEVSRMADRTMARIKEVETNTSSIQSAILESHT
                     QVLAAADSFGVMMRDLLQMGDSVSGFATRVDEQVSRVSAIAASMQQLSNAASTVEGAA
                     KESKNAARELAESTRTNASEADKILHESEALQEVSGNMRTRADQLGQIMDEFSLDESN
                     QASNR"
     gene            complement(2350..3603)
                     /locus_tag="PLG78_13830"
     CDS             complement(2350..3603)
                     /locus_tag="PLG78_13830"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNE24248.1"
                     /translation="MAESADELNPYLIACKQISRAAEILKLPRSVTEFILRPRRVLIM
                     NFPVRMDDGSVRVFEGFRAQHTDALGPAKGGIRFHPNVTLDEVKALSMWMTFKCGVVD
                     LPFGGGKGGVVCDPRSLSHGELERLSRAYAEAASVILGPEIDIPAPDVYTTPEIMGWM
                     MDTLSRLRGYNVPSAITGKPIVLGGSKGRDRATAQGCLYIVREYLRDRKIEPANARIV
                     IQGFGNAGRTAATLLSELECKIVAISDSKTGLQDPTGLNIQKACEAKDAGGLHLYGSE
                     FMIDSKLALQCEGDVLIPAALENSITSENAHHIQCKAVIEAANGPTTPEADKILSSRG
                     VQVIPDILANAGGVTVSYFEWVQNVTHSAWEETEVASRLEKRMIDAYHAVQKSARAHG
                     ADLRTAAYLISVERLRDAIVARGWA"
     gene            3682..>4137
                     /locus_tag="PLG78_13835"
     CDS             3682..>4137
                     /locus_tag="PLG78_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE24249.1"
                     /translation="MSIHLLKRRIPRGGELLPVIGLGTWQSFDKEPSEELSKVLATFY
                     EGGGKLIDSSPMYGKSEETVGSLIATRRKDFFVATKVWTRGEKQGFAQMEESFRKLQT
                     RKVDLMQIHNLVDWRIHLHTLRMMKEQGRIRYIGVTHYAPSSFGELTLAI"
BASE COUNT          909 a         1072 c         1032 g         1124 t
ORIGIN      
        1 gcatgcggac agccctttgt tcgtctcaag atagtcgtcc agcctcaagg aatatagaga
       61 aaactcatct tttacgtcgt ggaaccgagg ggacagctgc tcggctgcct tgtacaaaaa
      121 cgggcggaaa tacacattct gtctgtaatt gttgaatctg gctacctgga tgaggatgtt
      181 ggccatttcc ttttctgaaa gaggtggagt catccgcatc attttcgcgc tctggtgcag
      241 acgtttgaaa tgtctgtcga ttctgaaaat cagcagattg tccattcctt tggaatagta
      301 accgcgcact ccgccaaaga cggctgtacc gtattgcagg gcatgtgtgg ccacattcac
      361 ggtgcaatcg gatagctttt tgaattcact cttgtggtag gcgattgtgc tgagatccat
      421 gggcagaggc tcggagaccg cgttcaggcg tcaaccgtgg tttttgggca acgggccatc
      481 gctcctcagc ggttacttgc ctgattgctt tcatcgagtg aaaattcgtc catgatctgt
      541 cccagctggt ctgctctggt tctcatgttt ccggaaactt cctgcaacgc ttctgattca
      601 tgcaagatct tgtctgcttc tgacgcgttt gtacgagtcg attccgcaag ttcgcgtgcc
      661 gcgtttttac tctctttggc cgctccttct acggtggaag ctgcgttcga gagttgttgc
      721 atggatgctg caatcgcgct caccctcgat acttgttcgt ccactcgcgt tgcaaagccg
      781 ctgacagaat ctcccatctg aagcaagtcg cgcatcatca ctccaaagct atctgcggct
      841 gcgagcacct gagtgtggct ttccagaatc gcactttgaa tggaggaggt gtttgtttct
      901 acttctttga ttctggccat ggttcgatct gccatgcgcg aaacttcttc tgcaactacg
      961 gcgaatcctc gaccagcttc acctgctctc gcagcttcaa tggatgcatt gagtgcgagc
     1021 aggttgatgc ggtctgacaa ttcatgcatg agagacatga tcgcaccgat tttctgactg
     1081 ccatctttca cttgttccat ggattcagac acaatctgaa tttgttgtaa gccagagcgc
     1141 gcgcgattgg acgattccag gtttcgtgcg ccaagcgttt gcatggacat cctcatttct
     1201 tccgattcag tcgccagctg atgaatggaa tgatcgacct cttttgagtt ctgggccacg
     1261 acagtcatgg aatcggaaac atgatctgag atcgcagata gttcttctac cgccgccgct
     1321 gattcttccg aactcgccgc cagattttgc gaagccttca tcaatttctt agcagtaccc
     1381 gagatttcga aagctgcccc tctggtctgg tgaacttgat cgcgcacact tagaagcatg
     1441 gctgcaatct ttcgcgaact tctttttacc gcagagagaa ttgtgccgcg ttcatctgag
     1501 ctagagtcat ctatttctag attgagtttt ccttccgaaa tctgttgaag agttccggtg
     1561 accgcaccaa gagtgtttga aacgctgcgc accgccatcc aggaaaaaag aaacgtcagc
     1621 acggttacga tgaggcctgc actgcccaga gaaatccaga acagtcttag atccctctcc
     1681 tggtttcctt ttgactgatt agaaatgagt tctacaagtg cgctgagttg ttgatccgtt
     1741 ggtctatcca ttcctttcac acgacgatcc gcggcgcgcg taaaatctgg actaccagcg
     1801 gaaagttcct ccagtgtcac ttcgtattgc ttgagcaagt cggaatgcct tttctgtatc
     1861 tcgattgctt ttgatttcgt gtccgaattc gtttcgctct ctattagttt ttgaagaagg
     1921 gcctggaacg cagtggattc ggtgcgaaat gcgatcagat gcttcttgta tgcttccgga
     1981 tccgatccgc ggatgagtat gtttttccat tcctgaactt ggagtttgaa atggacctgg
     2041 ctttgtctca ccagttccat gcgcgctaca gcatcattca cgcgcgagga cagggccatc
     2101 tcgcttacgg ctacgaaaac gcctgcgaac aagaaggtcg caaagaagcc aagtgccagc
     2161 atccagattc gagcacggat gctcagccgc gaaataagca tggccaatga ctctcatgca
     2221 tgcgaaagtg gcaatcgttt tcgcacatgt gtcgcacccg tgtcgcacat ccttcgcacg
     2281 ccgttcgcgc actcgttgta cctgaagcca tggtttcgcg ccgccgttag gcgatattgc
     2341 gacagaatct caggcccaac ctcttgccac aatcgcatcg cgcaatcttt ctacggaaat
     2401 caagtaggcg gccgtgcgaa gatccgcccc gtgagctctc gctgacttct gtaccgcgtg
     2461 gtatgcatcg atcatccttt tttccagtct ggacgcgact tcagtctctt cccatgcgga
     2521 atgagtcacg ttctgaaccc attcgaaata cgaaacggtg actcctcctg cgttcgccag
     2581 tatatcagga atcacctgaa cgccgcgcga agagagaatt ttatctgcct cgggggtggt
     2641 tggaccgtta gccgcttcta ttacggcctt gcattgaatg tgatgcgcgt tttcggatgt
     2701 gatggaattt tcgagagcgg ctggtatgag aacatcacct tcacattgca acgcgagttt
     2761 actgtcgatc atgaactctg aaccgtacag gtgcaatcct ccggcgtctt tcgcttcgca
     2821 agctttttga atgttgagtc ccgtcggatc ctgtagacca gttttggagt cagagatggc
     2881 tacgatttta cactccagtt cgcttagaag ggtggctgcc gttcttccgg cattgccaaa
     2941 tccctggatc acaattcgtg cgttcgcagg ttcgatcttc cggtcgcgca ggtattctcg
     3001 cacaatgtag agacatccct gtgcggtagc acgatcgcgc ccttttgaac ctcctagaac
     3061 gattggtttt ccagtgatgg cagagggcac gttgtatccg cgcaatctgc tgagcgtgtc
     3121 catcatccag cccatgattt caggggtggt gtaaacgtca ggtgccggga tgtctatttc
     3181 cggtccaagt atcacagacg cagcttccgc atacgcacga ctcagtctct ccagctcccc
     3241 gtgcgaaagt gagcgaggat cgcatacgac tccgccttta ccgccaccaa atggaagatc
     3301 taccacgccg catttgaatg tcatccacat ggacagagct ttgacttcat cgagcgtgac
     3361 gtttggatga aatcgaattc ctcctttcgc gggcccaagt gcatccgtgt gttgcgcgcg
     3421 aaaaccttca aagacgcgga cgcttccgtc gtccatgcgc actggaaaat tcatgatcaa
     3481 cactctacga ggtcgcaata tgaattcagt gacgctcctg ggaagtttta gaatttcggc
     3541 ggctctggat atctgtttgc atgcaatcaa atacgggttc agttcgtctg cggattcggc
     3601 catatctgcc cggtttatct tcgccttgtt gaatcacaat ttttttctcg tctgtagcgc
     3661 aagcgttcgc acaaatgcgc catgtcgatt catctcttga agagaaggat tccgcgaggc
     3721 ggagaattgc ttccagtcat tggtctgggc acctggcaga gttttgacaa agagccttcc
     3781 gaggaactct caaaagtgct cgcaactttt tatgaagggg gcgggaagct gatcgattca
     3841 tctcccatgt atggaaagtc agaagaaacg gtgggctctc tcattgccac gcgtcgcaaa
     3901 gatttctttg ttgcgaccaa ggtatggaca cgcggagaaa aacaaggatt tgcacaaatg
     3961 gaggaatcgt ttcgcaaatt gcaaacgcgc aaggtggatt tgatgcagat tcacaacctg
     4021 gtggactggc gcattcattt acatactctt cgcatgatga aagagcaggg cagaattcga
     4081 tacattggag tcacgcacta cgctccgtct tctttcggag agcttacact cgccatc
//
LOCUS       DAOQJL010000346         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13140_length_4136_cov_4.545945, whole genome shotgun sequence.
ACCESSION   DAOQJL010000346 DAOQJL010000000
VERSION     DAOQJL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13140_length_4136_cov_4.545945"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLG78_13840"
     CDS             <1..399
                     /locus_tag="PLG78_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24250.1"
                     /translation="RGKSDVREATDLNQSHAVPERFHFLCAACGKIAGTMWLEEGQEC
                     NLVRESFTGRLTLPIDPQNRTPIGHWLRERNAQALHDFDFEIASFYCPSCDLNYCREH
                     WSARDVFDDDGWHDSIRGTCPAGHERMLED"
     gene            457..1671
                     /locus_tag="PLG78_13845"
     CDS             457..1671
                     /locus_tag="PLG78_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24251.1"
                     /translation="MILHLDPDEAYRSEIGTLLHANGLKSQGARSLGEALELVTRHAV
                     KVVVTELDHSDAHAEQILESLHRLDPSLAIIVLTSRPERRQGPTRRLGRVFEFVEKSQ
                     PPHILLSHIQRGRDYYLQRHHDNVIAGESEGKLRSKLEWLIWKQQAKLKVVDNYATLV
                     NNVKHSVAQGAGLDVMCTLVDLLMATATEVDGKMMIEAEVLPALNEAAASARRWLENL
                     DKMLSMTHLLHGKKDKTTAFHTSVRNAIASVHHLQIQKNQTIALGGLLECSVMMQQEG
                     FELCVRELLTNAFKYSPESSRIDITAHRAGDSASLTVLSDVMESKGNIDGIPAGSEEV
                     LFEPLVRLNNSYDERFRDEELAMGFGLAIVQSLVSSAGGRAYIREIRDFSVDFVARRR
                     VLSEIVVPILKN"
     gene            complement(1679..2350)
                     /locus_tag="PLG78_13850"
     CDS             complement(1679..2350)
                     /locus_tag="PLG78_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24252.1"
                     /translation="MSRLSEKIKIVYLTLVILFSMGVFAYLLDTWHIIKLKDHIPFLK
                     HEPPVVGEETDDASLRKEEQLQKEEQRLQELELKLKEAEAKLGEQTGNVQKQQEEIEL
                     MRKGLAEERKKLEEQKQAAFQRQKTVKEMSGRLLNMPPDDAVAICANWSNADVVDVFL
                     QMERDAEAEGRQSIVPFLLTKLPRERAGVITTLMMDERARMLPDTTDGPAPESAPSPA
                     PTPAP"
     gene            complement(2347..2526)
                     /locus_tag="PLG78_13855"
     CDS             complement(2347..2526)
                     /locus_tag="PLG78_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24253.1"
                     /translation="MSRWFTKDPIRERQMEIQRTRNDLEEFNVTIADLNRKIERMEKI
                     TAKWKERGESFDSRV"
     gene            complement(2540..3100)
                     /locus_tag="PLG78_13860"
     CDS             complement(2540..3100)
                     /locus_tag="PLG78_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24254.1"
                     /translation="MLKKRQIEQDLALKKLSVVLARYNKQEAARREAFELLKEETNLF
                     EKKYREEFELDLFQTYDRYLERLEANAADAARKMEEMQPELAAERELVMIARRNTRII
                     EKLKEHYKKDYEKENQLLEKEELFELNSRIAGMEEQHGESGIIEERRKPPEEPDSEDE
                     FPEDTAADLVSEYFRRLGLQDPRKKR"
     gene            complement(3132..>4136)
                     /locus_tag="PLG78_13865"
     CDS             complement(3132..>4136)
                     /locus_tag="PLG78_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNE24255.1"
                     /translation="HPLDMKEGITSSEERLHEGEPPSPHVRVPIRSPLETGVRAIDGL
                     LTIGRGQRIALMAGTGVGKSTLLGMICRYTRADINIVCLVGERGREVREFIENDLGEG
                     LEKSVVFVAESHRTAMEKVYCAGFATSVAEYFRDKGKHVNLIVDSLTRYCWALRDIGT
                     ASGEMLGPGGFPPNVWAKLSKLVERAGVTHEGSITGFYSVLVEGDDLNEPVTDHARGM
                     LDGHIVLSRRLAERGHYPAIEVTQSISRVMDKVISPEHLQDASRMKTLLSAYRENEEL
                     LNLGAYARGSNVDLDEYLDKASAIQGYLRQRVEEGVHIEDSVARLSDIIRPKIVEEAY
                     "
BASE COUNT          966 a         1094 c          990 g         1086 t
ORIGIN      
        1 agagggaaga gtgacgttcg ggaggccaca gatttgaatc aatcacacgc agtccctgaa
       61 cggtttcact ttctgtgcgc ggcctgcggc aaaattgctg gaactatgtg gctggaggag
      121 ggccaggagt gcaacctcgt gcgtgaatcg tttactggac gcctgactct tcccatagat
      181 ccgcaaaatc gcacaccaat aggccactgg ttgagagaaa gaaacgcaca agcactgcac
      241 gattttgatt ttgaaatcgc atctttctac tgtccgtcgt gcgacttgaa ctactgccgc
      301 gaacactgga gcgcacgaga tgtttttgat gatgacggct ggcacgattc gatccgcgga
      361 acctgtccgg caggacatga gagaatgttg gaagattgaa atctaaattg tccagggttt
      421 gtcttgacgc gcattggccc cggcgcatac agcacagtga tccttcacct ggacccggat
      481 gaagcctatc gatccgaaat cggaacgctg ttgcatgcaa atgggctgaa gagccaggga
      541 gcgcgatcgc tgggagaggc gctcgagctg gtcactcgcc acgcggttaa agtagtggtc
      601 actgaactcg atcactcgga tgcgcacgca gaacaaattc tagaatcgct tcatcgcctc
      661 gatccttccc ttgccatcat tgtcctcacg agtagaccag aacggcgcca gggaccaacc
      721 cgaagacttg gacgggtttt cgagtttgta gagaaatccc aacctcctca tatcctgctt
      781 tcgcacattc agagaggaag agactactat ctgcaacggc accacgacaa tgtgatcgca
      841 ggcgagtccg aaggaaaatt gcgaagcaaa cttgaatggc tgatatggaa acagcaggcc
      901 aaactcaagg ttgtggacaa ctatgctact ctggtgaaca acgtaaaaca ctcggtggca
      961 cagggcgcgg ggcttgatgt aatgtgcact ctagtggatt tgctgatggc aacagccact
     1021 gaggtagacg gaaaaatgat gatcgaggca gaagtgcttc ccgcgttgaa cgaagcggca
     1081 gcgtctgcac ggcggtggct tgaaaacctg gacaagatgc tgagtatgac acatctgttg
     1141 catggaaaaa aagataagac cactgcattt catacatcag ttcgaaatgc aattgcatcg
     1201 gttcatcacc tgcaaataca aaagaaccaa acaatcgcac tgggaggtct tcttgaatgt
     1261 agtgtgatga tgcaacagga aggtttcgaa ctgtgcgtac gcgagctctt gaccaatgca
     1321 ttcaagtatt ccccggaaag ttcgaggatt gacatcacgg ctcacagagc aggcgattct
     1381 gcttccctca cagtgctcag tgatgtaatg gaatccaaag gcaacataga cggtatcccg
     1441 gccggatccg aggaagtcct ttttgaacca cttgtcagac tcaataattc ctacgatgag
     1501 cgattccgcg acgaagagct ggcgatgggc tttgggctgg caattgttca gagcctggtt
     1561 tcttctgccg gaggacgcgc ctatataaga gagattcgcg acttcagcgt agacttcgtt
     1621 gcgcgaagac gcgttctttc cgaaatagta gtcccaatcc tgaaaaatta aaccggctct
     1681 atggagcagg agtgggcgca ggcgaaggcg cgctttccgg tgccggacca tccgttgtat
     1741 caggaagcat tctggcacgc tcatccatca tcaaggtcgt gattacgcct gccctttctc
     1801 ttggaagctt cgtgagaagg aaaggaacga tggactgacg accttcagct tccgcgtcgc
     1861 gctccatttg cagaaaaaca tccactacat ctgcgttgct ccagttcgcg caaatagcaa
     1921 ccgcatcatc tggaggcatg ttcaagagtc tgcctgacat ttccttgact gtcttttgtc
     1981 tctggaacgc cgcctgtttt tgttcttcca gtttcttgcg ctcttctgcg agaccctttc
     2041 gcataagttc gatttcttcc tgctgctttt gaacgttccc cgtttgctca cccagctttg
     2101 cttccgcttc cttcagtttg agctcgagct cctgcaagcg ctgctcttct ttctgcagtt
     2161 gctcttcttt gcgaagactc gcatcgtccg tttcttcacc cacaaccggc ggttcgtgct
     2221 tgagaaaagg aatatgatct ttcaacttaa tgatgtgcca cgtatccaga agatacgcga
     2281 atacgcccat ggagaataga atcaccaggg tcaaatagac gatcttgatt ttttcagaaa
     2341 gacggctcat accctgctgt caaaagactc gccgcgctct ttccatttcg cggtgatctt
     2401 ctccattctt tcaatttttc ggttcaaatc tgcaatcgtc acgttgaatt cttccaggtc
     2461 attccttgtg cgctggattt ccatttgacg ctcacgaatt ggatccttcg tgaaccagcg
     2521 agacaaaact ccttccgtgt tatcgcttct ttctcgggtc ctggagaccg agtcttctaa
     2581 agtactctga aacgaggtct gcggctgtgt cttcggggaa ctcatcctct gagtcaggtt
     2641 cttcaggagg cttgcgcctt tcttcaataa ttccactttc tccatgttgt tcctccattc
     2701 ccgcgattcg agaattcagc tcgaaaagct cttccttttc cagaagctga ttctcttttt
     2761 cataatcttt tttataatgt tcttttagct tttcaatgat acgagtgttt cttcgcgcaa
     2821 tcatcacaag ctctcgttcc gcagccagtt ccggctgcat ttcttccatt tttcgcgcag
     2881 cgtctgctgc atttgcttcg agtctttcca gatagcgatc gtacgtctgg aacagatcga
     2941 gttcaaactc ttcgcgatac ttcttttcga agagattggt ctcctctttg aggagctcaa
     3001 aggcctctct cctcgctgcc tcctgtttat tgtagcgagc gagaacgaca gacaatttct
     3061 tgagggccag atcctgttcg atctggcgct tcttaagcaa cgcttcgagc ggaaaacgga
     3121 acttcttttt tctaatacgc ttcctccact atttttggac gaatgatatc gctcagcctc
     3181 gcgacagaat cttcaatatg aacaccctct tctactctct ggcgaagata tccctgaata
     3241 gcggatgctt tgtccaggta ttcgtcgaga tccacattgg aaccacgggc gtatgccccc
     3301 aggttgagca attcttcgtt ttctctgtac gcagagagga gagttttcat tctgctggca
     3361 tcctgcagat gttcaggtga tataactttg tccatcacac gagaaatcga ctgagtaact
     3421 tcaatggctg gatagtgtcc ccgttccgcg agtctgcgag agagcacaat gtgtccatcc
     3481 aacatacctc gagcatggtc ggtcacgggt tcgttcaagt cgtcaccttc cacaagcacc
     3541 gaataaaaac ctgtaatgct cccttcgtga gtcacgcccg ctcgttctac tagcttgctg
     3601 agctttgccc agacgttagg cggaaatcca ccaggaccaa gcatctctcc gcttgcagtt
     3661 ccgatgtcgc gaagggccca gcagtatcgg gtcagtgaat ccacgatcag attcacgtgc
     3721 tttcccttgt ctcgaaaata ctctgccaca ctggttgcaa agccggcgca atagactttt
     3781 tccattgcgg ttctatgact ttccgcaaca aatacgacgc tcttctccaa accttcgccc
     3841 agatcgttct caatgaattc tctcacctca cgaccgcgtt cgcctaccag gcatacgatg
     3901 ttgatgtcag cgcgcgtgta tctgcagatc attccaagca atgtcgattt accgacgcct
     3961 gtgccagcca tgagcgcaat acgctggccg cgtccaatcg tcagaagtcc gtcaatggct
     4021 cggactcctg tttccagtgg actccgaatt gggacacgaa catgaggaga aggcggctcc
     4081 ccttcgtgaa gtcgctcctc cgaagaagtg attccttcct tcatgtcgag aggatg
//
LOCUS       DAOQJL010000347         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13145_length_4135_cov_3.750245, whole genome shotgun sequence.
ACCESSION   DAOQJL010000347 DAOQJL010000000
VERSION     DAOQJL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_4135_cov_3.750245"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLG78_13870"
     CDS             <1..263
                     /locus_tag="PLG78_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE24256.1"
                     /translation="VLKYIDPSYIIRSVPASADDAVFCIMLAQNAVHGAMAGKSGFMV
                     GYWNSYYTFVPLKLATSGRKKVDPKGYLWTVVCEATGQPDFF"
     gene            complement(287..2116)
                     /locus_tag="PLG78_13875"
     CDS             complement(287..2116)
                     /locus_tag="PLG78_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24257.1"
                     /translation="MNARLSTTTARELFSMQMHARLLPGIASYLTIVLTFVVLLPLEA
                     LRLFHTGFYAGDLLFAVEINLVAAAVIAALAQVLFFSMRILAALLMRFEWIAVSLLPG
                     VGVFLFFQAYVDSLATQHLPAVHPLIVWAATLTVGLLLSLPLLAVFPEIRGENSPLFL
                     FSVLLSRGFMLFSDVSGFLSTPVEVFLQFLVFCFLYIWLQLRRRIHRSPHYSPFVVPA
                     LQELGVFVVWLILYAASGVFPFVLPSFDTTVLTMATGIGISWSLGARFLGSQLQGAGG
                     GNAPWLAALSFILALGACVDLAIRYPVAAPSSSAHLLDSQTMAGRILLNAGVLLDEDN
                     DGNSVWPGGDPDDRDPCIRGDGLNVCKGSPPLDLPPPLHNRILLVTAASNEAEGRPLF
                     IPGDSMTVVLKAMLRGVDGYSAEMRKPSDPLLALISEYGFRTICVSVHWKDLLPESGN
                     GFDHGCQVVLEANDLKSAIDAAEKYSRKKFFVWIHLDRLDVGQTAVLKERISQYRYAI
                     IRMNLAKQTAYAETKDCPIPLHLRSILPALAADTEAPGDPVDVRSNSSVRAPALYRLL
                     GLHHARFFYSIKYSRKQDGTPYRRAFEGYTGAVWISEAGETID"
     gene            complement(2113..>4135)
                     /locus_tag="PLG78_13880"
     CDS             complement(2113..>4135)
                     /locus_tag="PLG78_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24258.1"
                     /translation="NFEESTLFVLGYYSHSDSRENSTSVLFGLYSNEKYPGGGYYTKY
                     LYGLVTSYEKSDGKAFWILPFYYSNQSLDGKETHWNVLGLINRTTDELSANLLVAPLF
                     YTRYGSADCEGKQRRTWAPFTYYQNTECGAARNSFTMLMFPFFYYDTDESHAAKSDSA
                     EVYFASLPGIYYHTSRTGEMREKTLFVLGYYSHSNSDEDNSSILLGLYSNETLKGGSR
                     STSFLYGLASFYSSPEESGSRILPFYTFNQSANGKQTHWNVLGFVNRSVDETSSNLLV
                     APFFYTSYGSPGCEGGKQRQVWAILGYYDDTDCGATQKSYSLYTPLYFGSANQSEQYS
                     SSWWFLPVPFLYRSSRADSTEKTQSFHTNLALLEDWESDEKGLRSVWSLPIVMWRRDD
                     YLFIFPYYHSEGTERSNHLLPLIYTWTTDTDFTGIFAGLYVRSYENYNRQNFLFLLDH
                     THREDYNEFEALLGAVHIANSRNQFDMKLLYGTLFSLRSSRDPSEFDLDFALYLGGLQ
                     FKPESTHSRLLPVYWYDHDRTSTFFASPVLTYDSSQTSRTVWSVPLLFYYNKDETSRT
                     IWSIPWIELESDSGARLFQLGLLGSVWYRNYDRYERTDRKLALLGIAYQDVERPERGY
                     RSRGSLWGLLWDYETEEETGFNKVSVLKVLYSRTENRGDVKHKVFGITL"
BASE COUNT         1184 a         1014 c         1036 g          901 t
ORIGIN      
        1 tagttctcaa atacattgat ccaagttaca tcatccgcag tgttcctgcc agtgcagacg
       61 acgcggtctt ctgcatcatg ctcgctcaaa acgcagttca cggagcgatg gcgggcaaat
      121 caggcttcat ggtgggatac tggaattcct actacacctt tgttccattg aagctcgcaa
      181 cgtctggccg caagaaagtg gatcccaaag gctacctgtg gacggtggta tgcgaagcaa
      241 ccgggcaacc ggattttttc tagaggaagg gcaaaggcga tcagactcag tcgatcgttt
      301 cgcctgcctc tgaaatccaa accgctcccg tatacccttc aaaagctcgt ctgtacggtg
      361 tgccatcttg cttacgcgaa tacttgatgg aatagaagaa cctggcgtgg tgcagcccca
      421 gcaatctgta gagggcagga gccctgaccg aagaattcga gcgaacatcc actggatccc
      481 ccggcgcctc cgtgtccgcg gccagagccg gcaaaatgga acgcagatgg agaggaatgg
      541 ggcaatcctt cgtctccgcg tatgctgttt gttttgctaa attcatgcga atgatcgcat
      601 aacggtattg ggagattcgc tcttttagaa cggcagtctg ccctacatcc agacggtcca
      661 gatgaatcca gacgaagaac ttcttgcgag agtacttctc tgcggcgtcg attgcagatt
      721 tgagatcatt tgcctccaga actacctggc atccgtgatc aaatccattt ccggattcgg
      781 gcaaaagatc tttccagtga acactcacgc agatcgtgcg aaaaccatat tctgaaatca
      841 atgcgagaag cggatcggaa ggctttcgca tctctgccga gtagccatcc acgccgcgaa
      901 gcatcgcctt gagaactacc gtcatggaat caccgggaat gaacagcggc cgcccctccg
      961 cttcatttga agcagcagtc acaagcagga tgcgattgtg caacggaggc ggaaggtcga
     1021 gaggaggact gcccttgcat acgttcagac cgtcgccgcg aatgcatgga tcacggtcat
     1081 ccggatcgcc gcctggccag actgaattgc cgtcattgtc ttcatccagg agaactcctg
     1141 cattgagcag gatgcgccct gccattgttt gcgaatccag caaatgtgca gatgaagatg
     1201 gtgcggcaac aggatagcga atcgcaagat cgacgcatgc tccgagtgca agaatgaaag
     1261 acaaagcagc caaccatggt gcattgcctc ctccagcacc ctgcagctgg ctccccagga
     1321 agcgggcgcc cagagaccag gaaattccaa tgccggtggc catcgtgagg acggtagtgt
     1381 caaaagacgg aagaacaaaa ggaaatacgc cggaggccgc gtacagaatc aaccagacta
     1441 caaaaacacc taactcttgt aaggcgggca ccacgaaagg cgaatagtgc ggggatctat
     1501 gaatccggcg cctgagttga agccagatat acagaaaaca gaagacaaga aattgcagga
     1561 acacttcgac aggcgtgctc aggaaaccgg aaacgtctga aaagagcatg aatccacgac
     1621 tgagcaagac tgaaaataag aacaaaggac tgttctcccc acggatttca ggaaagaccg
     1681 ccagaagcgg aagcgaaagc aaaagtccaa cggtcaatgt ggccgcccat acaatcagag
     1741 gatgaacagc tggaagatgc tgggttgcca gagaatctac ataagcctga aagaacaaaa
     1801 atacaccgac accggggagg agactcacgg cgatccattc aaaccgcatc agcagggcag
     1861 ccagaattcg catggagaaa aaaagcacct gagccagcgc agcgatgacg gcggccgcta
     1921 ctaaattgat ctccactgcg aaaagcaagt ctccagcgta gaacccggta tgaaagagtc
     1981 ggagcgcttc cagcggcaac agaacgacaa acgtcagaac aatggtaaga taggacgcaa
     2041 ttcctggaag caaacgtgcg tgcatctgca tggagaacaa ttcacgagcg gtagtagtgg
     2101 agaggcgggc attcaaaggg taatcccgaa gactttgtgt ttcacatctc cgcgattttc
     2161 cgttctgctg tacagtactt tcagcacgga caccttattg aatcctgtct cttcttccgt
     2221 ttcgtaatcc cagagaagtc cccagagact tcctctggag cgatacccac gttccggtcg
     2281 ttctacatcc tggtaggcga tacccagaag agctagcttg cgatcggttc tttcgtatcg
     2341 atcgtagttg cgataccaga cagatccaag caagccgagc tgaaaaagtc tcgcgcctga
     2401 atcggattcg agttcaatcc acggaatgct ccagatcgta cgcgaagttt catccttgtt
     2461 atagtagaaa agcaaaggaa cgctccacac tgtacgcgaa gtctgcgaac tatcgtacgt
     2521 caaaacagga cttgcgaaga aagtagaggt tcggtcgtga tcataccaat agaccggcaa
     2581 tagacgactg tgagtcgact cgggtttgaa ttgaagacca ccaagataga gagcaaaatc
     2641 cagatcaaac tcagacggat ctctggatga gcgaagagaa aatagcgttc cgtacagcag
     2701 tttcatatca aactgatttc tgctgttggc tatatgaacc gcaccgagaa gcgcttcaaa
     2761 ttcattgtag tcttcacgat gtgtgtgatc gagtaagaac agaaaattct gcctgttgta
     2821 gttttcataa gatcgcacgt acagtcccgc aaagattcca gtaaagtccg tgtccgtggt
     2881 ccaggtataa atgagaggaa gcaggtgatt ggatcgttct gttccttcgc tgtgatagta
     2941 tggaaatatg aacaaataat cgtctcttct ccacatcaca atgggaagac tccacacact
     3001 tcgcaggcct ttctcgtcac tttcccagtc ttccagcaaa gcgaggttcg tatggaacga
     3061 ttgtgttttc tcagtcgagt ccgcacgcga agagcgatac aggaatggaa caggcaaaaa
     3121 ccaccaggaa gaagaatatt gctctgattg attggcggat ccaaagtaga gcggagtata
     3181 cagtgaatag gatttttgtg tggccccaca atccgtatca tcgtagtagc cgagaatcgc
     3241 ccacacctgc cgttgctttc ctccttcaca cccgggtgaa ccgtaactcg tataaaagaa
     3301 cggagccacc agcaaattag aacttgtttc atccacgctg cgattcacaa agccaagaac
     3361 gttccagtgt gtctgcttcc catttgccga ttgattgaaa gtatagaaag gcaaaatgcg
     3421 cgaaccgctt tcctcaggtg aactgtagaa agatgcaaga ccatatagaa aactcgtgga
     3481 gcgagagcct cctttgagcg tttcgtttga atagagaccc agtaagatag aggaattgtc
     3541 ttcatctgaa ttggagtgtg aatagtaacc aagtacaaac aaagtctttt cgcgcatttc
     3601 gccagttcgc gaagtgtgat agtaaatgcc gggaagggag gcaaagtata cctccgctga
     3661 gtccgatttt gctgcgtgcg attcatccgt atcgtagtag aagaaaggaa acatcagcat
     3721 tgtaaaagaa ttccgcgccg ctccacattc tgtattttga tagtaggtga atggagccca
     3781 tgtacgacgc tgcttgcctt cgcaatcggc agacccatag cgagtataga acaacggcgc
     3841 aaccagcaga tttgctgaaa gttcatccgt agttctgttg atcagcccca ggacattcca
     3901 gtgagtctcc ttgccatcga gcgattgatt cgagtagtag aagggcagaa tccagaatgc
     3961 ttttccatcg gacttttcat aagaagtgac aagaccataa agatactttg tataatatcc
     4021 accgcccgga tatttctcat ttgagtacaa accaaacaag accgaagtag aattctctct
     4081 cgaatcagaa tgggaatagt atcccaatac gaaaagggtg ctttcttcaa aattg
//
LOCUS       DAOQJL010000348         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13154_length_4132_cov_4.767231, whole genome shotgun sequence.
ACCESSION   DAOQJL010000348 DAOQJL010000000
VERSION     DAOQJL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13154_length_4132_cov_4.767231"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1318
                     /locus_tag="PLG78_13885"
     CDS             98..1318
                     /locus_tag="PLG78_13885"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNE24259.1"
                     /translation="MEPVYVFDAVRTPRGKGKKRGVLAHIHPQNLAGDTLRALTKRNS
                     SLNAEKVEDVIMGCVSQVNEQGACVARYAVMAAEWPENVPGYTVNRFCGSGLQAINNG
                     VAAVGSGMSDLVVAGGVESMSRVKMGDDVGDLDMSIGNPFIAEHYNLVPQGISADLIA
                     TKYGFSREDVDRFAVESQQKADHAIKNGYFKNSIIPVKGPKGEDVLLDEHPRIESDYE
                     FLSGLGGVFKTYGESGADAIALKTYPDISKINHVHTLGNSSGVVDGAAVVLLGSEKAK
                     KEYGLRPRARIVSMAAIGSEPTIMLTGPVAASKLALQKAGLTAKDIDLYEINEAFAAV
                     VLFVQKELGIPKEKINVNGGAIALGHPLGGTGAILLGTLTDELERRNLRYGLATLCIG
                     GGMGIATVIERING"
     gene            1525..2550
                     /locus_tag="PLG78_13890"
     CDS             1525..2550
                     /locus_tag="PLG78_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24260.1"
                     /translation="MKHILLSALGLLSMPLYADGVKYVRSEFDANQYGVRALYVMFTN
                     EGNQPAKETVTARVKAKDGSTMNFRKEETIPAHAGLTFEITGMPADASEATIEWRGKA
                     VGTVKAGALPQAVVFPATGKDCSKSGVNIPHGARIRDKGKIQRCNNGNFQLDNTTFTG
                     FCPSSFSTSQQPNGIGGDAKFRSMDISPDRARVTCSYEVNASMTYRVSSNAHSCEGDP
                     KRPRHVTCTVPILGPIASLLEGREIGGRNFAERIKVQVLEYEKIQTMGLIDSKWISGN
                     QQDSSGQVELIFESLNVPSNNKSFTVTYKSTDARLQVNLPDKDFSGCKSAGKDKFTCE
                     ANVVKEF"
     gene            complement(2554..3561)
                     /locus_tag="PLG78_13895"
     CDS             complement(2554..3561)
                     /locus_tag="PLG78_13895"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE24261.1"
                     /translation="MRLCILDFNVWSGLTYQGMFHMQTYGDLDFRHRRRHSMIEQIRT
                     LNPDVVCLHELNPVPSEAAFIAKALDMDVFWHIHLAGIRLGPIGMPWNLYEGDAIFAR
                     KSLGLKALGRRKLSGGLVTRYASFNAQDATQILAAQIQCEDRVVPIFTTHWHAGVTPG
                     PDIEDRAKRIEKEEGVEPAKIQKALEMMRGNAGIRLKEAAWTVDFVDRTLERTQAENP
                     FAILSGDFNANPGTPEIRHLLDHGFADAYSAKHDDLGPTWDYAQNDHHKTFYSDYDPD
                     LYLRLNYTRLKIPHRLDYIFYRPSPTTTLLRCEIAMKDRIAGVQASDHFGVLAEFSLD
                     G"
     gene            3613..>4132
                     /locus_tag="PLG78_13900"
     CDS             3613..>4132
                     /locus_tag="PLG78_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNE24262.1"
                     /translation="MIGITTKPVADEKYGQSFEYVDAIIRAGGIPVLVPPVGNPAEIY
                     ERLDGLILSGGGDIDPSYYGGRAHSAIYGVSKERDEFEIELARVAARGRRPCLPICRG
                     IQVLCVALGGTLIEHIPDETDESIVHRDPDMKEGKPHKVDITPNSILALALEATSCTV
                     ISWHHQAIRDPGP"
BASE COUNT         1119 a         1042 c         1057 g          914 t
ORIGIN      
        1 cgatggagcc tggcgccggt tttctggacc caacagacag acttgtctgt tcaagaaacc
       61 acccgcgggt gatgcaggca agcaaaaagg agcgcttatg gaaccagttt atgtattcga
      121 tgcggttcga acaccgcgag gcaaagggaa aaaacgggga gtgctggcgc atattcatcc
      181 gcagaacctg gccggggaca cactgcgcgc acttacaaag cgcaactcat ctctgaacgc
      241 agagaaagta gaagatgtaa ttatgggctg cgtatcgcag gtaaacgagc agggagcctg
      301 tgtggcgcgc tacgctgtta tggcggcaga atggccagag aacgttcctg gttacactgt
      361 aaatcgtttc tgtggatccg gtctccaggc aatcaacaac ggcgtggccg cagttggatc
      421 gggaatgtct gatctggtgg tggcaggtgg agttgaatcc atgagccgcg taaagatggg
      481 cgatgacgta ggcgatctgg acatgagcat tggaaacccg ttcatcgcag agcactacaa
      541 cctggttcca cagggaattt cagcagacct gattgccact aaatatggat tcagccgcga
      601 agatgtggac cgtttcgcag tagaatcgca acaaaaagca gatcatgcaa tcaagaatgg
      661 atactttaag aatagtatca ttcctgtaaa aggaccgaag ggagaagatg ttctcctgga
      721 cgaacatcct cgcattgaaa gtgactacga gtttctttcc ggactcggtg gcgttttcaa
      781 aacatacggc gaaagcggtg cggatgcaat cgcactgaag acgtatcctg acatctcaaa
      841 gatcaatcac gtgcacactc tgggcaactc ttcaggcgtt gtagatggag cggctgtggt
      901 tcttcttgga agcgaaaagg caaaaaaaga atacgggctg agacctcgcg ctcgcatcgt
      961 atcgatggct gccattggat ctgaaccaac catcatgctg accggtccgg tggctgcatc
     1021 caaactggct ctgcagaaag cgggactcac tgcgaaagac attgatctct acgaaatcaa
     1081 tgaagcattt gcagcggttg ttctctttgt tcagaaagaa cttggaattc cgaaagagaa
     1141 aatcaacgta aacggcggag ccatcgcact gggccatcca cttggaggaa ctggcgcgat
     1201 tctactggga actctgacag atgaactcga gcgcagaaac ctgcgttacg gcctggccac
     1261 tctctgcatc ggcggtggaa tgggcattgc aaccgtaatc gagcgcatca acggctgatt
     1321 cgattttcat cagacaaacg caaaaccacg gagaaatccg tggttttttt gtgccagaga
     1381 accacctcct aaatgggact gaccctttca tattgcgctg gcaaccctca gaacgcgcag
     1441 aaaatccttg atctccgttc tgccggtgaa aagattttta ccagcatccc gacctgttcg
     1501 tctaaccaca ttgtaggaga atatatgaag cacattttac tgtccgcact cggccttcta
     1561 agcatgcctt tgtacgcaga tggagtaaaa tacgttcgct cagaatttga cgccaaccag
     1621 tacggagtca gggcgctcta tgtgatgttc acaaatgaag gcaaccagcc ggcaaaagaa
     1681 acagtaactg caagagtaaa agccaaagac ggcagtacaa tgaacttcag aaaagaagaa
     1741 actataccgg cgcatgcggg cctgactttt gaaatcactg gaatgccggc agacgcaagc
     1801 gaagcaacca tcgaatggag aggcaaggcc gtgggaaccg ttaaagccgg cgcgctgcct
     1861 caggcggtcg tctttcctgc tacaggaaaa gactgtagca agtcaggcgt aaacatccct
     1921 catggtgcgc gcatcagaga caaaggaaag atccagcgct gcaacaacgg taatttccag
     1981 ctcgacaata ccacttttac aggattttgc ccttcctctt tcagcacatc acaacagcca
     2041 aatggaatcg gtggtgatgc taaattcaga tcgatggaca tttcacctga ccgtgcacgc
     2101 gttacatgtt cgtatgaggt gaatgcgtcc atgacataca gagtttccag caacgcacac
     2161 tcgtgtgaag gagatcccaa acgcccgcgc cacgtgacgt gcaccgttcc tattcttgga
     2221 cccatcgcaa gtcttctgga gggccgcgaa atcggcggaa ggaatttcgc agagagaatc
     2281 aaagtacagg ttctggagta tgagaagatc cagactatgg gcctcattga ttcgaaatgg
     2341 atttcaggca atcaacagga ttcaagcggc caggtggaat tgatttttga aagcctcaat
     2401 gttccgtcca acaacaagtc ctttaccgtt acttacaaat ctacggacgc aaggctgcag
     2461 gtgaaccttc cagacaaaga cttttctgga tgcaaatccg ccggcaaaga taagttcacc
     2521 tgcgaagcca atgtggtgaa agaattctaa agttcatcca tccagagaaa actcagcaag
     2581 tactccaaag tgatcgctgg cctggacacc agcgatcctg tccttcattg cgatttcgca
     2641 tcgcagtaac gtggttgtgg gcgaaggtcg atagaagatg taatcgagac gatgaggaat
     2701 cttcagcctc gtataattca aacgcagata tagatcaggg tcatagtccg aatagaaggt
     2761 cttgtgatga tcgttctgag catagtccca cgtcggaccc agatcgtcat gcttcgcaga
     2821 atacgcatcc gcaaaaccat ggtccagcaa gtgccgaatt tccggcgttc ctggattcgc
     2881 attgaaatca ccggatagaa ttgcaaatgg gttttccgcc tgggtacgct ccagagtgcg
     2941 atccacaaaa tcaaccgtcc acgccgcttc cttgaggcga attcctgcat tacctcgcat
     3001 catttccagg gccttctgga ttttcgcagg ctccactcct tcctctttct ctattctctt
     3061 cgcgcgatcc tctatatcag gacccggagt gactcctgcg tgccagtgag tggtaaagat
     3121 cggaaccacg cgatcctcgc actgaatctg ggccgccagg atctgagttg catcctgcgc
     3181 gttgaagctc gcataacggg ttaccagacc accgctcagc ttccgtcttc ccagagcttt
     3241 cagtccgagt gacttgcgtg cgaagatcgc atctccttca tacaaattcc acggcatccc
     3301 gattggacca agacgaatgc ctgccaggtg aatgtgccag aatacatcca tatccagagc
     3361 cttcgcaatg aaggccgctt ccgatggaac tggattgagt tcgtgcaagc aaacaacgtc
     3421 cggattcaat gttcgaattt gttcgatcat ggaatggcga cgcctgtgcc tgaagtccag
     3481 gtctccatac gtttgcatat gaaacattcc ctggtaagta agaccggacc atacgttgaa
     3541 atcaagtatg caaaggcgca cgcgaaatga tcctggatgg gcccggagga tcaatcgaaa
     3601 ttgtggagac ctttgatcgg aattacaacg aagcccgttg cagatgaaaa gtacggacag
     3661 tcttttgaat atgtagatgc aatcattcgg gccggaggca ttcctgttct tgttccgcca
     3721 gtcggaaatc cagcggagat ctacgaacgc ctggacggcc tgatcctgtc aggtggagga
     3781 gacatagatc catcttacta cggcggccgg gctcattctg cgatttacgg agtaagcaaa
     3841 gaaagagacg aattcgaaat cgaactcgcg cgcgttgcag ccagaggaag acgtccctgt
     3901 ctgccgattt gccgcggaat ccaggtactc tgtgtagccc tcggcggaac tctcatagag
     3961 catattccgg atgaaactga cgagagcatt gtgcatcgtg atccggacat gaaagaagga
     4021 aaaccacaca aggtagacat aacgccgaat tctattctcg ctcttgcact cgaagccaca
     4081 tcgtgcaccg taatctcctg gcaccaccag gcaattcgcg atcccgggcc tc
//
LOCUS       DAOQJL010000349         4130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13167_length_4130_cov_4.756810, whole genome shotgun sequence.
ACCESSION   DAOQJL010000349 DAOQJL010000000
VERSION     DAOQJL010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13167_length_4130_cov_4.756810"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="PLG78_13905"
     CDS             <1..1885
                     /locus_tag="PLG78_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24263.1"
                     /translation="KVQSLSTPQAARSGAYFTVGPIAFDGIGQIPRKELAAVLKNLTR
                     DRLVVQVLRFEFENGAIVFKPCFLARPQDRPESMRQIYEEIISQSATAFEKWMIQSPV
                     TAVQLAREMQVDLSAGKEFSFNSLTSFLSLDTFVNVRLFDVLPDSEMIEAVLGDIRTS
                     LLERNLCVYVAGYGILKLAGANAADLFDAASDFMIDRVAPRYRNRGNCRRELAQIAVE
                     EGIYHIDEFVPETPDFLVCRAEELRRVGPGDPPESPNGRYAGAMAVEIVVALAPTVKE
                     KYLADWRAENERSIENIKKKLTGVNAAWLDLMSFTDQKDLMKMNPANWNRLVGDESLL
                     YGAWELPRTTMHILVHRKSSVFRRIVRGMVDSVAPREWQVLALKDLIETYEDYFKDLF
                     LDREFVQMYGQLLRRAYLPYMNWFFRLLVALNIKLFQDVAFQGAKKRIARHQAVLEAR
                     NHERYLELMKQREEERFKKTNQIKRMALSNRMIDRLDYYYFQEGSLPTVGRLRAELGN
                     ISEEDFEALLKEERFQVITMNRGDSRDDRLLLYPLDGGWKAKSARLAEALDAQEKEYD
                     RPDADEFQKKVLTSIRGLSRVVKKSPSIRLPESGMDPYERLEKEIRLYKERRRSSPEE
                     LEV"
     gene            complement(1888..2502)
                     /locus_tag="PLG78_13910"
     CDS             complement(1888..2502)
                     /locus_tag="PLG78_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE24264.1"
                     /translation="MARKENASVIQTASAKRPPLTKKLILDAALKIIETEGMEKLSMR
                     RIAALLDCSVAGPYVYFTNQEEILKALIDDGEARLTAELKAAVQKKVDIFDKIFILMQ
                     TYKSFASANRELHKLMFAMGGRGKSFSLISPSYRVYFETVRQGAKNGAIRCDRAGYHA
                     IARAMWSWGYGLVVLELARHPETKKSEIDPIAEGMELFKKLLQP"
     gene            complement(2561..4039)
                     /locus_tag="PLG78_13915"
     CDS             complement(2561..4039)
                     /locus_tag="PLG78_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE24265.1"
                     /translation="MARRIKPIDQLLSETEKEALHRNLGPFSLIALGVGAIIGAGIFI
                     LTGNAAAGENLSTTSLLHAPVLDIIMGGLDIQSHGRLPAGPGISVSFLITGLICALAA
                     LNYAELASMIPVAGSTYTYAYATLGELIAWIIGWDLILEYAVSNMAVAVGFSAYFNDV
                     LESIFGIRLPPELSRPLIENGAMTGAWFNLPSFIILMILTILLVRGVRQSAHTNNLMV
                     LIKLGAIVLFIIGGAKYIRFEHYHPFFPGGFSGVMAGAAIVFFTYFGFDSVSTAAEES
                     RNPARDMPIGIIASLIVCALLYVSVAIVLTGIADYRTLNNPAPVAGALKALGLDSIRS
                     LVGTGAVVGIISCLLVFQYGQARIWYAMSRDGLLPAAFSRVHPKFRTPHISTWIAGLV
                     VGIPAGIWDIGTFADLANIGTLAAFVIVSAGVLILRRTEPDRKRGFRVPFVPWLPILS
                     IVTCAFLMISLPLETWIRFFVWMAIGLVIYVLYGRHRSSMAQ"
BASE COUNT         1046 a          992 c         1113 g          979 t
ORIGIN      
        1 gaaggtgcag tctctttcca ctcctcaggc ggctcgaagc ggagcctatt tcacagtggg
       61 tcccattgca tttgatggca ttggtcagat tccacgcaaa gagctcgcag cggttctaaa
      121 aaacctgacg cgcgatcgtc ttgtggttca ggttcttaga tttgaatttg aaaacggtgc
      181 gattgtcttt aagccatgtt tcctcgcgcg acctcaggac agaccggagt ccatgcggca
      241 gatttatgag gagattattt ctcagtcagc cactgcattt gaaaaatgga tgatccaatc
      301 gcctgtgact gcggtacagc tggcccggga gatgcaggtt gatctgagcg cgggaaagga
      361 gttttctttc aattccttaa ccagctttct gtcgctcgat acgttcgtaa atgttcgcct
      421 cttcgacgta ctacccgatt cggaaatgat cgaggcagtt ctgggcgata ttcgaacttc
      481 tctgctcgaa agaaatctat gcgtgtatgt ggccggttac ggaatactga agctcgctgg
      541 agccaatgct gcggatcttt ttgacgctgc gtctgatttc atgatcgatc gggtggcgcc
      601 gcgctatcgc aaccggggaa actgtcgccg cgaactcgcg cagattgcgg tggaggaagg
      661 gatttaccat attgacgagt ttgttccgga gacacctgat tttctggttt gccgcgcaga
      721 agaattgcgg agagtgggcc ctggagatcc accagaatcg ccaaacggcc gttatgcggg
      781 cgcgatggca gtggaaattg tcgtggctct ggcccccact gtaaaagaga agtatctggc
      841 agactggcgc gcggagaacg aaagaagcat tgagaacatc aaaaaaaagc tcacaggcgt
      901 gaatgcggcg tggctggatt tgatgagctt cacggatcag aaagatttga tgaagatgaa
      961 cccggcgaat tggaatcgtc ttgtgggaga tgaaagtctt ctgtacggcg cctgggaact
     1021 gcccagaacc acgatgcaca ttctggtgca cagaaaatca tctgttttta gaaggattgt
     1081 ccgcggcatg gtagattccg ttgcgcccag ggaatggcag gttctggctt tgaaggatct
     1141 catcgaaaca tacgaggact atttcaaaga tttatttctg gatcgcgagt tcgttcagat
     1201 gtacggccaa cttttgcgga gagcctatct tccgtacatg aactggttct ttcgattgct
     1261 ggttgcattg aatatcaagc tctttcaaga cgtagcgttt cagggcgcga agaagagaat
     1321 cgctcgtcat caggccgtgc ttgaagcgcg aaaccatgaa cgttatctgg aattgatgaa
     1381 gcagagagaa gaagaacgat ttaagaagac caatcaaatc aagagaatgg ccctttcgaa
     1441 tcgtatgatt gatcgtctgg attactacta ttttcaagag ggtagtcttc ccactgtagg
     1501 acgccttcgc gcagaactgg gaaacatttc agaagaggat ttcgaggcgc ttctcaaaga
     1561 agaaagattt caggtaatta caatgaaccg tggggattcc agagacgatc ggcttttgct
     1621 ctatcctctg gatggaggct ggaaggcaaa gtccgcgcgt ctggctgagg ctcttgatgc
     1681 gcaggaaaaa gaatacgatc gcccggatgc ggacgagttt cagaagaaag ttctcacctc
     1741 tattcgcggc ctttccagag ttgtgaagaa atctccgtcc attcgactgc cggagtcagg
     1801 gatggacccg tatgaaaggc tcgaaaagga aattcgacta tataaagaga ggaggcgttc
     1861 ttcgcccgaa gaactggaag tttagtctca gggttggagc agtttcttga acagttccat
     1921 gccctctgca atcggatcaa tttcggattt cttagtttct ggatgacgcg caagctcgag
     1981 caccaccaga ccgtaacccc aggaccacat ggcgcgcgcg attgcgtgat agcctgcgcg
     2041 atcgcaacgg atagctccat tttttgcgcc ctgtctgacc gtttcgaaat agacgcgata
     2101 actgggtgaa atcagggaaa agctttttcc tctcccaccc attgcaaaca tgagtttgtg
     2161 tagctcgcgg tttgcggatg cgaacgattt gtatgtttgc attagtatga aaattttatc
     2221 aaaaatatca acttttttct gtacggcggc cttgagttct gccgtgagtc ttgcttctcc
     2281 gtcgtctatg agtgccttga gtatttcttc ttgatttgta aagtatacgt aaggtccggc
     2341 cacgctgcaa tccaggagtg cggcgatgcg cctcatgctg agcttctcca tgccttctgt
     2401 ctcaatgatt ttgagagcgg catccaggat gagttttttt gtaagaggcg gtcgctttgc
     2461 ggaggctgtc tggatgacgg acgcgttttc tttgcgggcc attgcgtcta atgcaccttt
     2521 ctcatgcaaa gctcaagtag aatccggcca gatgccactt ttattgcgcc atcgaactgc
     2581 gatgcctgcc gtagagcaca taaatcacaa gtccaatcgc catccatacg aagaaacgaa
     2641 tccaggtctc cagcggaaga gagatcatca ggaatgcaca cgtgactatg gatagaatgg
     2701 gaagccatgg aacaaacgga actcgaaagc cgcgctttct atctggttct gtccgtcgta
     2761 gtatcagtac gcccgcagaa acaatcacaa acgcagccag tgtccctatg tttgccagat
     2821 ctgcaaacgt gccaatgtcc cagatccccg ccgggattcc aaccaccagc cctgcaatcc
     2881 aggtgctgat atgcggagtg cgaaattttg gatgaacgcg cgagaacgcc gcaggaagaa
     2941 gaccgtcacg cgacattgca taccagatgc gggcctggcc atattgaaat acgagcaggc
     3001 aggaaataat gcccacaacg gctcccgttc caaccaggct tcgaatggaa tcaagaccca
     3061 gcgctttgag tgctcctgcc acgggcgccg gattattcag agtgcgatag tccgcgatcc
     3121 ccgtcaaaac aatggccacg gaaacgtata acagcgcgca gacgatcaga gaagcaatga
     3181 tgccgatcgg catgtcgcgt gcaggatttc tggattcttc tgccgccgta gaaacagaat
     3241 caaacccaaa gtatgtaaaa aagacaatgg ccgcgcccgc catgaccccg gaaaaaccgc
     3301 ctggaaagaa tggatgatag tgctcgaagc gaatgtattt tgcgccacca atgatgaaca
     3361 acacgatggc tccgagcttg atgagaacca tgagattgtt tgtatgtgcg ctctggcgaa
     3421 cgccgcgaac cagcagaatc gtcagaatca tcagtatgat aaacgaaggc agattgaacc
     3481 acgctcccgt catcgcgccg ttttcaatga gtggtcgcga aagttcaggc gggagacgaa
     3541 ttccaaaaat cgactccaga acatcgttga agtaagcgga gaaaccaacc gcaacagcca
     3601 tattggatac agcgtattcc aggatcagat cccatccaat gatccaggcg atcagttcac
     3661 caagcgtcgc gtaggcgtag gtgtatgtgc tgccagccac gggaatcatg gaggccagtt
     3721 ccgcgtagtt gagtgcagcc agagcgcaaa tgagtccggt gatgaggaag ctgactgaaa
     3781 tcccgggtcc cgccggaagt cttccgtggg actgtatgtc cagtccgccc atgatgatgt
     3841 ccagaacagg ggcgtgcaaa agggaggttg tgctcagatt ttccccggct gctgcatttc
     3901 cagtcaggat aaaaatcccc gctccaatga tggctcccac tcccagggcg atcaggctaa
     3961 atgggcccag gtttcggtgc agagcctctt tctccgtctc tgaaagcagc tgatcgattg
     4021 gtttgatgcg acgggccatg cagttcactg gtcctgaaat gattgcctgt caaagcaaat
     4081 ttggcattcg tgtagtatgc agaatatagt gcagcccgca gaagccggac
//
LOCUS       DAOQJL010000350         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13200_length_4124_cov_3.868027, whole genome shotgun sequence.
ACCESSION   DAOQJL010000350 DAOQJL010000000
VERSION     DAOQJL010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13200_length_4124_cov_3.868027"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLG78_13920"
     CDS             complement(<1..265)
                     /locus_tag="PLG78_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24266.1"
                     /translation="MQSSEMDFTFMQKRIEDAVTDNLKTITNLKVDAGAAVKEILSYV
                     FRNVNPDSVLADHARYDWYYSLLGKTIAIAQGWKINHAPEVKSA"
     gene            complement(311..2434)
                     /locus_tag="PLG78_13925"
     CDS             complement(311..2434)
                     /locus_tag="PLG78_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNE24267.1"
                     /translation="MGTVYRSCTLCEAICGLEIKVEDGVIKGIRGDSADVFSHGHICP
                     KGPELKAIHEDPDRLKFPLKRVGDKWEQVAWETAFSEIAARLHVIQTASGQDSVAYYA
                     GNPNVHNYGSLLFAPRLMKAIKTRNIYSATSVDQLPHHFAALKMFGHPLLVPVPDVDR
                     TDYFLILGANPMASNGSMMTAPDMRGRMKAIQKRGGKIVVVDPRKTETADLADEHVFV
                     RPGKDVYFLLSILHVVLAGEKKKTSRLESIFQGADFVRSLVQDYSPEKTQAHTGISAE
                     TTRRIATQFAQAKSAVAYGRIGVSTQTFGGLCHWLINVLNAITGNLDEPGGAMFSLPA
                     VDTIAAEGKRESFGSFDSYRSKARNLPEFGGELPVSALADEILFQGEGGVRALITSAG
                     NPVLSTPNGGKLAQALESLQLMVSIDIYLNETTKYAHYILPPTSPLEHDHYDIVFNML
                     AVRNTARYSPPVFDPPAGTMHDWEIFTALTAAMEEARSGGAKKASSARAKIKPTDFLD
                     RMLKSGPYGSNGLNFQKLVENPHGVDFGPLVPVLPARLKTIDGQIDLAPDLMRRDIER
                     AREAMKETPAGMILIGRRNLRDNNSWMHNVPRLMNGSPRCTLFIHPEDAAKFGISDGH
                     EAIVRSRVGEIRLPVEIVEEIMPGVVSIPHGYGHKKSGTKMSTAQKHAGVSINDLTDD
                     SSLDELTGNAAFNGVPVEIRAASVS"
     gene            complement(2437..>4124)
                     /locus_tag="PLG78_13930"
     CDS             complement(2437..>4124)
                     /locus_tag="PLG78_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE24268.1"
                     /translation="KLVRPVGHQGGDKDYLTSIRVTWDDSEHGMGPTGMAIKTGLCHV
                     NQDVYTNPAMAPWRKEAISRGYISTIALPLQDASGTFGALNIYAGEANAFDDDEIELL
                     RKLAADLSFGVEALRTREKRRRAEEAVHRLAYFDTLTGLPNRTQLLASLTNGIAESAL
                     KAQSLALLFFNIDHFTEFQDGLGVRQAEALLQKIALRLQSAAGSNFLARLDGDRFAMI
                     HPNATADSISALAVRVHRAMEESIVQSDVRIDVRINLGAAMYPDHGADADSLLLRSDI
                     ACRRAKALGVSHMMYSGATDQESPHRLSLIADLRRAIQSDQLILHFQPKIDSRTGVIS
                     GAEALVRWSHPVRGLISPAEFIPLAEHTGLIAPLTDWVLRAAVRESAAWLAKGIAVPI
                     AVNVSPQNLRDDDLIDRISTLLQEAGVPAENLQLEITETTLMEDPARSFELLSRLKKQ
                     GIRIFVDDFGTGYSSLSYIATLPLYSLKIDRSFVVKMTRSKEHRAIVAAAISLAHSLG
                     LRVVAEGVDAVDQLADLKELGCDEIQGFLFSKPLSSKDFLSWSVDFSNTAVRR"
BASE COUNT         1018 a         1105 c         1008 g          993 t
ORIGIN      
        1 ctgcagattt cacttctggc gcatggttga ttttccaacc ctgagcaatg gcgatggtct
       61 ttccaaggag tgaataatac caatcgtaac gcgcatgatc cgcaagcaca gaatcgggat
      121 ttacgttcct gaaaacatac gagagaattt ctttgactgc cgctcctgcg tccaccttga
      181 gattcgtgat tgtctttaga ttgtccgtca ccgcatcttc aatacgcttc tgcatgaatg
      241 taaaatccat ttctgatgac tgcataaagc gatgaggaat gattcgcagt tcccgtccag
      301 caaaacgcaa tcaagaaaca gaagcagcgc ggatctccac aggtacacca ttgaacgcgg
      361 cgttgcctgt gagctcatcc agggatgaat cgtccgttaa atcattgata ctcacaccag
      421 cgtgcttctg agccgtgctc atctttgtgc cagatttctt gtgtccatac ccatgcggga
      481 tgctcacgac accaggcatg atttcctcta caatctcaac gggcagcctg atttcgccga
      541 cacgagatcg aacaatcgct tcgtggccgt ctgaaattcc aaacttcgca gcatcctctg
      601 gatgaatgaa aagagtgcat cgcggtgaac cgttcatcag gcgcggtaca ttgtgcatcc
      661 acgaattgtt gtcgcgaagg ttccttctgc cgatcaaaat cattccggca ggcgtttcct
      721 tcatggcttc gcgcgctcgc tctatgtcgc gcctcatcaa atccggagcc agatcaatct
      781 ggccgtctat tgttttgagg cgagctggca ggacgggcac tagaggtccg aaatcaactc
      841 cgtgcggatt ctcaaccaac ttttgaaaat tgagcccatt gcttccgtaa ggtccggatt
      901 tcaacatacg atccaggaaa tcagtgggtt tgatcttcgc tcttgctgac gaagccttct
      961 ttgccccgcc gctgcgagct tcctccatag ctgctgtgag tgcagtaaag atctcccagt
     1021 catgcatggt gcctgcgggc ggatcaaata caggagggga gtaacgagcg gtatttctca
     1081 ctgccagcat gttgaataca atgtcgtaat gatcgtgttc gagaggagaa gtaggcggca
     1141 aaatgtagtg agcgtacttc gttgtttcat tcagatagat gtcaatgctc accatcagtt
     1201 gcagtgattc aagtgcctgc gccagcttac cgccgttagg tgtagaaagc acgggattgc
     1261 cggcggaagt aatcaaagcg cgaacgccgc cctctccctg aaacaagatc tcatctgcca
     1321 gggcagacac aggaagctct cctccgaact ccggtaaatt gcgagcctta ctcctgtatg
     1381 aatcaaaact tccaaacgac tcccgctttc cttctgcggc aatggtatcc acggccggca
     1441 aactgaacat agctccacct ggctcgtcca gattgccagt gatagcgttc agaacattga
     1501 tgagccagtg acagagaccg ccaaacgttt gagtcgaaac tccgattcgg ccataagcca
     1561 cggctgattt tgcctgcgca aattgagtgg caatccgcct cgtggtttcc gcagaaattc
     1621 ctgtgtgagc ctgggttttt tctggagagt aatcctgaac caggcttcgc acaaagtcgg
     1681 caccctggaa gatgctttcg agtctggagg tcttcttctt ttctcctgca agcacaacgt
     1741 gaagaatcga aagtaagaaa taaacgtctt tgcccggacg aacaaagaca tgctcgtctg
     1801 ccaggtccgc cgtttccgtt ttgcgcggat ctactacaac aattttccca ccgcgcttct
     1861 gaattgcttt cattctgccg cgcatgtcag gagcagtcat catactaccg ttagacgcca
     1921 ttgggttagc acccagaatc agaaaatagt ctgttcgatc cacatctggc acagggacga
     1981 gaagaggatg cccgaacatt ttgagagcag cgaagtgatg cggaagctga tctacggaag
     2041 tggcagaata gatgttcctg gtcttgatcg ccttcatgag ccgcggtgca aacagaagag
     2101 aaccgtaatt atgtacgttt ggatttccgg catagtaggc aacggaatcc tgtccgcttg
     2161 cggtctggat gacatgcaat ctggccgcaa tttcggaaaa tgcagtttcc cacgcgacct
     2221 gctcccattt gtctcctact ctcttcagtg gaaatttcag acgatctgga tcctcatgaa
     2281 ttgctttgag ttcagggccc ttgggacaaa tgtgtccgtg cgaaaagaca tccgcgctgt
     2341 ctccgcgaat tcctttgatc actccgtctt ctactttgat ctcaagcccg caaattgctt
     2401 cgcatagcgt gcacgaacga tacactgttc ccatgatcat ctcctaacgg ctgtattcga
     2461 gaaatcgaca ctccaggaca aaaaatcttt tgaagacagt ggctttgaaa atagaaatcc
     2521 ctggatctca tcgcatccaa gttccttcaa atcagccagc tgatccacag cgtccacacc
     2581 ctctgccacc actcgcaaac ccagagaatg cgccagagaa atggccgcgg ccacaatcgc
     2641 acggtgttct ttgctcctgg tcatcttgac cacaaaggaa cgatcaatct tcagggaata
     2701 caaaggaagg gttgcaatgt agctgaggga agaatagcca gtgccaaaat cgtccacgaa
     2761 gattcggatc ccctgcttct taaggcgcga aagcaattca aaggatcgag cgggatcttc
     2821 catcagagta gtttctgtga tttccagctg caagttttca gccgggacgc ccgcttcttg
     2881 aagcagtgta gaaatgcgat caatcaaatc gtcgtcgcgc aagttttgtg gagacacgtt
     2941 gactgcgatg ggaactgcaa ttccttttgc aagccacgct gcgctctctc ttaccgcagc
     3001 acgcaaaacc caatcggtca gaggagcaat caggccagtg tgttctgcaa gcggaatgaa
     3061 ttctgcaggt gatataagtc cgcgaactgg atgggaccag cgaaccagtg cttctgctcc
     3121 actgatgaca cccgtgcgag aatcgatctt tggctgaaaa tgcaaaatga gctgatcgga
     3181 ttgaatggca cgcctgaggt ctgcgatgag agaaagcctg tgaggacttt cctggtccgt
     3241 ggcgccactg tacatcatat gcgaaacgcc cagcgctttc gcgcgacggc atgctatatc
     3301 gctgcgcaaa agcaaggaat ccgcgtctgc accgtggtcg gggtacatag ccgcacccag
     3361 attgatgcgc acatcaatgc gaacgtcact ctggacaatg gattcttcca tggctctgtg
     3421 aacgcgcacg gccagagcag agatggagtc tgcggtggca ttcggatgaa tcatcgcaaa
     3481 ccgatctcca tccagtctgg ccaggaaatt ggatcctgcg gcagattgaa gtctaagagc
     3541 gatcttctgc aaaagcgctt ccgcctgacg aactcccagc ccatcctgaa actcggtaaa
     3601 atgatctata ttaaagaata acaaagcaag agactgcgcc ttgagtgcgg attccgcaat
     3661 tccattggtc aaactcgcaa gcagctgcgt tcgattgggc agaccggtga gagtgtcaaa
     3721 atacgcaagc ctgtgaacgg cctcctctgc ccggcgtctt ttttcgcgcg tgcgtagcgc
     3781 ctccactcca aaactcagat ccgccgcgag cttgcgcagc agttctattt catcatcatc
     3841 aaaggcattg gcttcccccg cgtaaatatt cagtgccccg aacgtgccgg aagcatcttg
     3901 cagcggtaag gcgatcgtag aaatataacc cctggaaatc gcttcttttc tccagggtgc
     3961 catggctgga ttggtgtaca catcctggtt cacatgacac agccccgtct tgattgccat
     4021 tccggttggc cccatgccgt gttccgaatc gtcccaggtt acgcgaatcg aagttagata
     4081 atccttatcg ccaccctgat gaccaacagg gcgcacaagc ttct
//
LOCUS       DAOQJL010000351         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13216_length_4120_cov_4.529397, whole genome shotgun sequence.
ACCESSION   DAOQJL010000351 DAOQJL010000000
VERSION     DAOQJL010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13216_length_4120_cov_4.529397"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3901
                     /locus_tag="PLG78_13935"
     CDS             <1..3901
                     /locus_tag="PLG78_13935"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNE24269.1"
                     /translation="ATQTTDANGHATLANLSEFTREKAPVAFIAKAGSDLSFMPFARD
                     DRKLDFSRFDVGGLQGADSRDKLTAFIFSDRGMYRPGDEIRAGLIIKSGDWKRNLSGL
                     PVEVSILDPRGIEVFSRLIKLSPSAFEEIKYKTEETSPTGSYQIRAMLVPPKKYQERI
                     TLGQASVRVEEFLPDKLRITTQFSQTPQDTEGWVKPEQLKGQVLLMNLFGTPAQHKKV
                     QATLVLTPRFPAFSGYEGYRFYDPLRTTRSFQQDLGTLETDDQGKVEFPLGLEKYAKG
                     LYWVTFHADGFESEGGRAVSTQSLQMVSPLSAVVGIKSDGDANYIHKGSARAVSIIAI
                     DEKLKPVSLSKLKLRIVEQRPMSSLVKREDGVYEYKSIVKEIPVLDRDLIIPQGGFNQ
                     LLPTDQSGDFVLSIVDESSQELNRFQYSVIGQGNLTRSLERNAELSIRLSGKDFSPGE
                     EIEMAIRAPYVGAGLITIEKDKVYAYKWFKTATESSVQRIRMPEGLEGNAYVNVSFVR
                     SLDSKAIYTSPLSFGVAPFSVSKKSRTAAVTLNVPAEIRPGDTLRIQYRTDRPGKIVL
                     YAVDEGILQVAKYRTPDPLGHFFQKRALEVSTSQIVDQLLPEFSIVRSLMKMGGDADR
                     LIGLNLNPFKRKRALPVAFWSGIRDSAGAGEVSFTVPDYFNGRVRVFAVLVNESVLGV
                     EEKSVIVRGDFILSPSVPAFSAPGDTMNVSTNVANNVIGSGNDLPVRVEARAEGSLEI
                     VGDNTAALRISEKGEKTARFQVRAKSPGEGRIIFMATSAGKAGKQTETISVRPARPYI
                     LDVESGYLAGGSDSFESKRTLYPDFRKLEVSVSPVPLAWSSGLFAYLENYPYGCTEQI
                     TSRGFAALVLRNRPELARGDNDKIISNTISVLRARQNDSGSFGFWAPNSYVDPFQSTY
                     ALLFLTEAKERGVTVPDAVLSKGMNYLESMSSSPKDATLHDRAFALYVLARNGKLHAN
                     EVNSLLIEASREKDDFVRLPYMYLAATRKLMKQDKEATDLFEKSQPGVFSRWIFNSFD
                     PVSANASYMYLLSRHFPGSFETLSDKTMQSFFSPMKQGQFSTISAASMILAIDAYLDL
                     RHARRLDLKVTQELGSGEKRPLLFPDGLFAKSVFLPEARKIKFDAGSPLPAFYQVTVG
                     GFDEKLPAPYANGIEISREFTDQSGNKIQSAEAGQEVYVRIRIRAKDTDASNIAIVDL
                     FPGGFEPVFDEKTRTDTVGIPGFKPALMEPDHVEMREDRIVLFTRATSRASEYTYRLR
                     AVSAGKFVVPPLFAEAMYQKSTRALTGEGQFEIKTRK"
     gene            3898..>4120
                     /locus_tag="PLG78_13940"
     CDS             3898..>4120
                     /locus_tag="PLG78_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24270.1"
                     /translation="MTDFIRAFWKRRSKIFKRAAIGAACYVLLRIVSAGGPLSGMPFS
                     RAVYDADGKLLRLTISTDQKYRKFTSLADM"
BASE COUNT         1104 a         1009 c         1034 g          973 t
ORIGIN      
        1 ggccactcaa accacagacg caaacggaca tgcgactctc gcaaacctca gcgaattcac
       61 gcgagagaaa gcgccagtcg ctttcatcgc gaaagccggc tctgacctgt ctttcatgcc
      121 atttgcaaga gatgatcgaa agctggactt ttctcgcttt gatgtgggag ggcttcaggg
      181 tgcggattca cgcgacaaac tcacagcatt tatcttctcc gaccgtggca tgtatcgacc
      241 cggagacgaa attcgcgcag gcctgatcat caagtccgga gactggaaga gaaatctttc
      301 cggtctgcct gtggaggtga gcattcttga tccgcgcgga atcgaagtgt tttccaggct
      361 gatcaaactt tctccgtctg catttgaaga aatcaaatac aagacagagg aaacttctcc
      421 caccggttct taccagatcc gggccatgct tgtgcctccc aagaaatacc aggagcgaat
      481 cacactcggt caggcaagtg ttcgcgtaga ggaatttctt cccgacaaac ttcgcatcac
      541 aactcaattc tcgcaaactc ctcaagacac agaaggatgg gtgaagccag aacagctcaa
      601 aggccaggtg ttgctgatga acttgttcgg aacacctgcg cagcataaaa aagtacaggc
      661 cacactcgtc ttaactcctc gttttcctgc gttcagcggt tacgaaggtt acaggttcta
      721 tgatcctctt cgcacaacgc gaagcttcca gcaagatctg gggacgctcg aaaccgacga
      781 tcagggcaaa gtggaattcc cactcggact cgaaaaatac gcgaaaggat tgtactgggt
      841 tacttttcac gcggatggtt ttgaatctga gggaggacgc gcagtcagca cacaatcact
      901 gcaaatggtt tccccgcttt cagcggtagt cggaatcaaa tcagatggag atgcaaatta
      961 cattcacaaa ggatccgctc gagccgtctc catcattgca atcgacgaaa agctgaagcc
     1021 tgtctctctc tccaaattga aactgcgcat tgtagagcag cgcccgatgt ccagtctggt
     1081 gaagcgcgaa gatggagtct acgaatacaa gagcatcgtc aaagagattc cagtgctcga
     1141 tcgtgatttg atcatcccgc aaggaggatt caatcaactg ctgcccacgg atcagtctgg
     1201 cgatttcgtt ctgagcatcg tggacgaatc atcgcaggaa ttgaaccgct ttcaatattc
     1261 agtgattggt cagggcaatc taacacgaag tcttgaaaga aatgcggagc tttccatacg
     1321 actgagcggc aaagatttct ccccgggaga agagattgaa atggcgattc gcgctcccta
     1381 cgttggggcg ggtctcatta ctatagagaa agataaagta tatgcgtaca aatggttcaa
     1441 aactgcaact gaatcctcag tgcagagaat cagaatgcca gaaggcctgg aaggaaacgc
     1501 gtacgtaaac gtttcttttg tgcggtctct cgattcaaag gcaatttaca caagcccact
     1561 gagctttggg gtcgctccat tctcagtcag caagaagagt agaacagcag cagtcacttt
     1621 gaatgtgcca gcagaaatcc gccccggaga tacactgcga atccagtatc gcacagacag
     1681 gccgggcaaa attgtactct atgcagtaga cgaaggaatc ttacaggttg caaagtacag
     1741 aacaccggat cctctgggcc acttctttca gaaacgagcc ctggaagtca gcacatcgca
     1801 gatcgtagat caactccttc cggagttttc catcgtgcga agtttgatga agatgggagg
     1861 agatgcggat cgcttgatcg gtctaaatct caaccctttc aaacgaaaga gggcattgcc
     1921 tgtagcattc tggtctggaa ttcgtgattc ggcgggagcg ggagaagtaa gcttcacagt
     1981 tcctgattat ttcaacggta gagtgcgcgt atttgcagta cttgtaaacg agtccgtcct
     2041 tggtgtagaa gagaaaagcg taatcgtgcg cggagatttc atactgtctc cttccgtgcc
     2101 tgcgttttct gcaccggggg acacgatgaa cgtgagcacg aatgttgcca acaatgtgat
     2161 cggatcggga aatgatctgc ccgtgcgcgt agaagcgcgt gcggagggat cgcttgagat
     2221 cgtaggagac aatactgcgg cactgcgcat ctcagaaaaa ggagaaaaga ccgcccgttt
     2281 ccaggtgcga gccaaatcgc ctggagaagg aagaatcatt tttatggcaa catccgccgg
     2341 aaaagcaggc aaacaaacag aaacaatcag tgttcgtccc gctcgcccgt acattctgga
     2401 tgtagaatct ggatatctgg caggtggatc ggattcattt gaatccaaac gaacgcttta
     2461 tccagatttt cgcaaactag aagtatctgt atctcctgtg cctcttgcct ggtcttctgg
     2521 tctgtttgct tatctggaga actatcctta tggctgcacg gaacaaatta caagccgggg
     2581 atttgcagca cttgtgcttc gaaatcgacc agaacttgcg cgaggagaca atgataagat
     2641 catctccaat accatttctg tccttcgcgc gcggcagaat gattctggat cgtttggatt
     2701 ctgggcgcct aattcttatg tagatccgtt tcaatccacc tacgcgttgt tattccttac
     2761 ggaagcaaaa gagcgcggtg tgaccgtgcc agatgcagtt ctgagcaagg gtatgaatta
     2821 cctggaatcc atgtcctctt ctccaaaaga cgcgactttg cacgacagag cgtttgctct
     2881 gtatgtgctc gcgcgaaatg gaaagctgca tgcaaacgaa gtgaacagtc ttttaattga
     2941 agcgtccagg gagaaagatg attttgttcg cctgccctac atgtatctgg cggccacgcg
     3001 caaactcatg aaacaggaca aagaagcaac agacctcttt gagaaatccc aaccaggggt
     3061 attcagtcgt tggattttca actcatttga tccagtgtcc gccaatgcaa gttacatgta
     3121 tcttctatcg cgtcacttcc ctggcagttt tgaaacgtta agcgacaaga ctatgcagag
     3181 cttcttttct cccatgaagc aaggtcagtt cagcacgatc tccgccgcgt ccatgattct
     3241 ggccattgac gcatatctgg atctgcggca cgcacgcaga ctggatttga aagtgactca
     3301 ggaacttggc agcggagaaa agcgtcctct cctgttcccg gatggtctgt ttgcaaaatc
     3361 cgtcttcctt ccggaagcgc gcaagatcaa gttcgatgca ggatcgccgt tgcctgcgtt
     3421 ctatcaggtc accgtgggcg gattcgatga aaaacttcca gcaccgtacg caaacggaat
     3481 cgaaatttcc cgcgagttta cagatcaaag tggaaacaag atccaatctg cagaggccgg
     3541 ccaggaagtg tatgtaagaa tccgaatccg cgcaaaagac acggatgcat ccaacattgc
     3601 cattgtggat cttttccccg gaggtttcga accagtcttc gacgaaaaaa caagaacaga
     3661 cactgtgggc attcccgggt tcaagcccgc attgatggaa ccagatcatg tggaaatgcg
     3721 cgaagacaga attgtacttt tcacaagagc cacatcacga gcatccgaat atacatacag
     3781 attgcgcgcg gtgagtgcgg gcaagtttgt tgttccacct cttttcgcag aagcaatgta
     3841 tcaaaaatct actcgggcgc tcactggaga aggacagttc gaaatcaaaa ccaggaaatg
     3901 acggatttca ttcgagcctt ctggaagaga aggtcaaaga ttttcaagcg cgcagcgatt
     3961 ggcgctgcgt gctacgtttt gctcaggatt gtttctgccg gtggacctct cagcggaatg
     4021 cctttctcgc gcgcggtcta cgacgcagac ggtaagctac ttcgcctgac tatttccacg
     4081 gatcaaaagt atagaaagtt taccagtctc gcagatatgt
//
LOCUS       DAOQJL010000352         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13226_length_4118_cov_4.028058, whole genome shotgun sequence.
ACCESSION   DAOQJL010000352 DAOQJL010000000
VERSION     DAOQJL010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_4118_cov_4.028058"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLG78_13945"
     CDS             complement(<1..799)
                     /locus_tag="PLG78_13945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNE24271.1"
                     /translation="MIAVRYRSLELEPGTYMKWISRLLVLCFSLTSVLSCIKIQASPF
                     DTSNGGYLGALATYLATLSQRGIAFSDSAMGMESRGDGSWTAFSFTGPAKIVRVAAVG
                     TTLLGLDGSTAGVLWKSTNYGRSWTAQGGLGAVAFQQIAVCGAGVLLVQTPGGSLGAY
                     YSSDSGVTFSPVTVNATSALTVLDATCINNVFYVSHSGPPTNVSRSTNGSTWVLGSPA
                     PSSAVAVLSGTGSNLFGQGSGSPLVFSKSTDGGLSYSAGDSVANFSAM"
     gene            complement(806..1171)
                     /locus_tag="PLG78_13950"
     CDS             complement(806..1171)
                     /locus_tag="PLG78_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24272.1"
                     /translation="MKKINLAKVVLMCALVATASACKKEATEVVATGTVLALKGVISE
                     TLAASDMKKAGIAFAAFDATWENVEDGVKAASPNAYKDIENGMDEVNIAIKKNDAAKL
                     KEAGAKLLKTLDDNAAAFK"
     gene            complement(1168..2631)
                     /locus_tag="PLG78_13955"
     CDS             complement(1168..2631)
                     /locus_tag="PLG78_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HNE24273.1"
                     /translation="MRTVCLAFWVVSALAWPAFASPVEDMRELDSILARSEEALARGD
                     LKAARAQYELCGDRWFAVEDGVKHQSRPAYKAIESAMGDVRFAFTEPGRPDEIRRSLG
                     ALRNANAAFLAGKFGASQEDSGGKQIAQATVPELLVILSQVSESIERKDAARAKLEFD
                     SFQSGWPDVEGFIGTKSRDDYVLIENHITTAQAALRSTPADYQIASRSVEILKDKLRL
                     YSASQRYTMLDAATILIREGLEALLVIVALAAFLEKSGSANRKSWIWGGSLVGVVVSI
                     LSGVLIHRLFAGFVTNSNRELVEGLTGLFAAAMLLYVSYWLHSKSSLASWQNYIKDQV
                     TSALARGSLLSLGFLAFLAVFREGAETTLFYIGIAPAISPVDLFSGIGIGVAVLTLAA
                     ILMLFVGLRIPLRPFFLFTGLLIYYLGFKFVGSGVHALQIANVLSVNSSEHLWSIPSF
                     GVYPTWETSVPQLMLLILLATAVLYSRMRKNTSGGTT"
     gene            2767..3969
                     /locus_tag="PLG78_13960"
     CDS             2767..3969
                     /locus_tag="PLG78_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNE24274.1"
                     /translation="MSLKRFPLICASVIAIVATCSVESSPYDVALSSYSPVTPLREVR
                     ALWIVRDSIKTTESIRAIVESMRRNKFNTAIVQVRGRGDAYYRSEFVPVAAGIAPGLD
                     PLAEFIRVAKPYGISVHAWINVFLGADRESLRTAPKNHLAFAHRDWFLRDRSGRSMLT
                     YSAREMRAADVEGAFLDPSNQDVRRYNVQVVHELLSKYPVDGIHLDYIRYPSSQTGTP
                     FDFGINSLLPTDPRKAPDILAMRKARAENVTQMVSEIREEIRRIGPSRILSAAVWPNK
                     MKVEERVFQMWPEWLRSRLIDYAFLMAYYDTETLHDQRVSQFFDPEINNRMIIGVGIY
                     KNPAPKVALHQLTSARAMGAAGICYFHAEWFLSKNAIEKEKKYQLPEVFDTWKDPGFT
                     PARTSSIP"
BASE COUNT         1071 a         1134 c          994 g          919 t
ORIGIN      
        1 tcatcgcact gaagttcgca acagaatctc cagcgctata gctcaaaccg ccgtctgttg
       61 actttgaaaa aacaagagga gatccactgc cctgcccgaa caggttcgaa cctgtgccgg
      121 aaaggacggc aaccgcagac gacggcgccg gacttccgag cacccacgta ctaccgttcg
      181 tagagcgact aacgttcgtt ggcggaccgc tgtggcttac ataaaagaca ttgttgatgc
      241 atgttgcgtc cagaacggtc aatgcagaag tagcattcac agtaacagga gaaaaagtca
      301 caccactgtc agacgaataa taggcgccca gggatccacc tggagtctga acaagcaaga
      361 ccccggctcc gcaaactgca atttgctgga acgcaaccgc tcccagccca ccctgcgcag
      421 tccaggatct tccatagttt gtggatttcc agagcacgcc tgcggtgctt ccgtccaacc
      481 ccagcaaagt ggtgcccaca gcggcgactc gaacgatctt cgccggtcca gtgaaagaaa
      541 acgcagtcca tgaaccgtca ccgcgggatt ccattcccat agcggaatcg gaaaatgcaa
      601 tgccgcgctg agagagggtt gccagatacg tagcaagtgc tcccaggtat ccaccgtttg
      661 aagtatcaaa cgggctggcc tgaatcttga tgcaggataa aacggaagtc agtgaaaagc
      721 agagaaccaa aagccgggaa atccatttca tataggtgcc cggctctagt tcaaggctgc
      781 gataccgtac tgcaatcaca aaatgctatt taaatgcggc tgcgttatcg tctaacgtct
      841 tgagcaattt tgcccctgct tccttgagtt ttgccgcatc gttcttcttg attgcgatgt
      901 ttacttcatc catgccgttt tcgatatcct tataggcatt aggcgatgct gccttgaccc
      961 catcctccac attctcccag gttgcgtcaa aggcggcaaa agcaatgccc gctttcttca
     1021 tatcactggc ggccagagtc tctgaaatca ctcctttgag cgcaagcaca gtgccagtcg
     1081 ccacaacctc tgtcgcctct ttcttgcagg cagatgcggt tgccacgagc gcgcacatca
     1141 aaactacttt tgccaaattt attttcttca tgtggttcct cctgaagtat tctttctcat
     1201 tcgcgaatat agtacagccg tagcgagcag gatcaaaagc attaactgag gaacgcttgt
     1261 ctcccaggta gggtaaacgc caaaagacgg aatggaccac aggtgctcgg aagaattcac
     1321 ggacagcaca tttgcgatct gcagtgcatg cactccactg cctacaaact tgaatccaag
     1381 gtagtagatc aggagccccg taaaaaggaa aaacggacgc agcggaattc gcagaccgac
     1441 aaagagcatc agaatagcgg ctaacgtcaa cacggcgact ccaattccaa tgccagaaaa
     1501 aagatccaca gggctaatgg cgggagcaat cccaatatag aaaagagtcg tctcagctcc
     1561 ttcgcgaaac accgcaagaa acgccagaaa tccaagagag aggagacttc ctcgtgcaag
     1621 agcggaggtt acctgatctt tgatataatt ctgccacgac gccagggatg acttgctatg
     1681 aagccagtaa ctgacataca aaagcatggc ggccgcaaac aaccctgtca ggccttctac
     1741 aagttcacga tttgaattcg taacaaagcc agcgaaaaga cgatggatca agacaccgga
     1801 caaaattgaa acgacgactc ccaccaatga accgccccag atccaggact tccgattcgc
     1861 tgaaccactc ttctccagga aggctgccag tgccacaatg acgaggaggg cttcgagacc
     1921 ttcgcggatc aagatcgtgg cggcgtcgag catcgtataa cgttgcgacg cagagtatag
     1981 tcgcagtttg tctttgagta tttccaccga acggcttgct atctgataat cggcgggcgt
     2041 ggaacgaagc gccgcctgcg cggtcgttat gtgattctca atcagaacgt agtcatcacg
     2101 agatttggtt ccaatgaagc cctccacatc cggccaacca gattgaaacg aatcaaattc
     2161 taattttgcc ctggccgcgt cctttcgctc tatgctttca gatacttgcg aaagaatcac
     2221 cagcagttcg ggcaccgtag cctgagcgat ttgtttgccc ccggaatctt cttgcgatgc
     2281 gccaaacttg cctgcgagga aagcagcgtt tgcattgcga agagctccca gcgagcgacg
     2341 gatttcatct ggtcgcccgg gttcggtaaa cgcaaagcgc acatccccca tcgcactttc
     2401 tatcgctttg tatgcaggac gcgattgatg cttcactccg tcctctactg cgaaccagcg
     2461 atcgccacac aactcgtact gcgcccgggc tgccttcagg tctcctctgg ccagtgcttc
     2521 ctcagatcgc gccaggatgg aatccagctc acgcatatcc tccacagggg aagcaaacgc
     2581 gggccacgcc agagcagaaa caacccagaa ggccagacaa acagtgcgca tgagacaaga
     2641 ttctgaaatg gggcacggca ggcaagccat tctgatatta agaatcagtc tcaattagat
     2701 cgggattgcg gtccgttccc aattctggtt gaatcctgcg gccaccaggg ttgcctggag
     2761 tgccccatga gcttgaaacg attcccactg atttgcgcaa gcgtcatagc cattgtcgca
     2821 acctgcagcg tggaaagcag tccgtatgat gtggcgctga gttcttattc acccgtaacg
     2881 ccacttcggg aagttcgtgc tctctggatc gtacgcgatt ctatcaaaac cacagagtcc
     2941 attcgtgcca tcgtggaatc gatgcgaaga aacaagttca ataccgcgat cgtccaggta
     3001 agaggccgcg gcgacgcata ctatcggtcc gagttcgttc cggtcgcagc cggaattgca
     3061 ccagggcttg accctctggc agaattcatt cgcgttgcaa aaccttacgg catatctgtt
     3121 catgcgtgga tcaatgtttt tctgggagca gacagagaaa gcctgcgcac tgcgcccaaa
     3181 aatcacctgg cctttgcaca cagggactgg tttcttcggg atcgcagcgg cagatccatg
     3241 ctcacctatt cagcaaggga aatgcgcgca gcggatgtgg aaggtgcatt tcttgatcca
     3301 tctaaccagg acgttaggcg atacaatgtg caagtcgtcc acgagcttct gtcaaagtat
     3361 ccggtagatg gaattcatct ggattacatt cgataccctt cttcccagac gggcactccc
     3421 tttgacttcg gaatcaattc actcttgccc actgatccca ggaaagcacc tgacattctg
     3481 gccatgcgta aggctcgagc tgagaacgta acgcagatgg tatctgaaat tcgagaagag
     3541 atccgacgca tcggaccatc gcgcattcta tctgccgccg tatggcctaa caagatgaag
     3601 gtggaagaga gagtatttca aatgtggccg gaatggcttc gatcgagact cattgactat
     3661 gcgttcctca tggcttacta cgacacagag actctacacg accaacgagt atcgcaattc
     3721 ttcgatccag aaatcaacaa cagaatgatc atcggtgttg gaatttacaa aaatcctgcg
     3781 cctaaagtag ctcttcatca actgacgagc gcccgtgcga tgggagcggc cgggatctgc
     3841 tactttcatg cagaatggtt tttgagtaag aacgcaattg aaaaagaaaa gaaataccag
     3901 cttcccgaag tattcgacac gtggaaagat ccaggcttta ctcctgcgcg cacatcgagc
     3961 attccctgat tctaatcacg caagatctta aattccgttc gcctgttctt cgcatacgct
     4021 tcttctgttt ctcctggatc cactggctcc gtctgccctt tgccgtccgt cttcactctg
     4081 tcaggtgaaa caccacgatt tactagttcg cgcttcac
//
LOCUS       DAOQJL010000353         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13259_length_4110_cov_4.589642, whole genome shotgun sequence.
ACCESSION   DAOQJL010000353 DAOQJL010000000
VERSION     DAOQJL010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13259_length_4110_cov_4.589642"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLG78_13965"
     CDS             <1..996
                     /locus_tag="PLG78_13965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24275.1"
                     /translation="RNSILFVSVFLLLGSLVFAFLISGRLSRPIESLASAAERIAGGE
                     LGVTVQITTAGEIGQLGATFNHMSRSLKDSERLKTEQAAIKRELEIAQGIQVAMIPEP
                     QKVGPYSFRGFMQTADEVGGDYVDVIHIDGRSPRFWFFIGDVSGHGLRAGLTMLMAQT
                     AIHSTMEVSPDLEPSQAFMHVNDVLFANIRRLRERKYMTAAFYRADAKGNFSFAGLHQ
                     DALIYRAAGKKLEVVPTDGFWLGVDAGVKKDMRAGKFKLSVGDLLFLFTDGLVESMNG
                     KKEMFGQEKIEEIVRKHGTSDLAGLEERIMNSLRAHTGGLRPADDITFAMIRREK"
     gene            998..1465
                     /locus_tag="PLG78_13970"
     CDS             998..1465
                     /locus_tag="PLG78_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24276.1"
                     /translation="MSFSLTIEPEWNVIKKIKESLQTDPALKGLSKDFLEQTTVTAIE
                     LLENALKYTDSDSGRPVEFSFDSQAGYCAFRVKNYSKNAQNKDALRSVIDRIRDGDPF
                     ELYVARLEQLKEDPDGHSRMGLYRIAYEGEYHLSADIDGESVTILARRPLENA"
     gene            1465..1833
                     /locus_tag="PLG78_13975"
     CDS             1465..1833
                     /locus_tag="PLG78_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24277.1"
                     /translation="MALSNLTMQDFEIEAVVSGSNIELNWKGAMHAPNPEDVLDPYFE
                     QVIAEALKAKLGVKCNFVPLEYMNSASIPPLIALLRTLADKKIQGEFVYDSNRKVQSA
                     SFRALDVIARKSKYTSVRGI"
     gene            1833..3038
                     /locus_tag="PLG78_13980"
     CDS             1833..3038
                     /locus_tag="PLG78_13980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24278.1"
                     /translation="MVDVRDLWQESPSAELVLRDGKLMAMNPAAEKLLGITASDLGDS
                     VWEERVFDEKARKKFRSFLESQAPVSSGLDASGADGSIRHIRFLKRNIPSHGTLLSVQ
                     DVTETREDAEAFQAGYDEFIRVTTDLEDALATIEKQNRQLERQKQILEGELQVAHAVQ
                     AQILSQDFEKHTLVNVFGTYQAMTELGGDMWEFFESENGTWVVIADVMGHGVASSLIS
                     IAAKSIFKRRFEEASSGKMDLAELVKAANRDLCELTHSNYFLTACAVHIDSNHRLEYL
                     TAGHPPVLYVPADSSRQVELLFTEQPMLGVFRNIEYHSVPFQMFEGDRIFIYTDCLIE
                     SVNAEGDVIDLLQIADRLRLKPGTTPQTVVQGMLEYRREFAGSDRLPDDLTMVCIEIP
                     QAAGLRVAK"
     gene            3035..>4110
                     /locus_tag="PLG78_13985"
     CDS             3035..>4110
                     /locus_tag="PLG78_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNE24279.1"
                     /translation="MIRVYACILTAAMLCAAGLIAERRHIAVIVSSDTGIYADALTGF
                     YSSFDPGLAQIDQPVYVDALPDPARFFSERSDADLIVTIGSKATQLALAEGHGSKIVF
                     SMVSSPRAVGVASRPDVCGTSMDVSLSEFFKSLRELSPSARNVYAFYSTPEGEFAASE
                     GEYLDLQLGVEYHKRRLSGKSELPRALESIQGKADAFYLVPDALYDSEQFQIVSDFCK
                     KNKIVLMTSFSSLMQAGATFSLRPDYTRIGRVTAEMAGTVLQGKGCGPRRVVFPDQAY
                     FSLNERYAEESGLTIPQSVKETASRTRLFLSAVNLMNEGKPKSALIVFDAILKKAPDD
                     AAAKRYRDAIVERQSGAQTRQMMES"
BASE COUNT         1015 a          959 c         1121 g         1015 t
ORIGIN      
        1 cgcaactcca tcctgtttgt ctctgtcttc ttgcttctgg gttcacttgt atttgcattt
       61 ctcatttcag gaaggttatc gagacccatc gaatcgcttg catccgcggc ggagaggatc
      121 gcgggcgggg aactcggcgt tacagttcag atcacgactg caggagaaat cggacagctg
      181 ggcgcaacct tcaatcacat gtcgcgcagt ttgaaggatt cggagagact caagacggag
      241 caggcagcca tcaagcgaga gcttgagatt gctcagggca ttcaagtggc catgattcca
      301 gagccgcaaa aggtcggccc ttattcgttt cgaggcttca tgcagactgc agatgaagtg
      361 ggcggagatt atgtggatgt catacacata gacgggcgct cgcccagatt ctggtttttc
      421 atcggagatg tttcaggtca cggtcttcgc gcaggtctaa ccatgctcat ggcgcagact
      481 gccattcatt ctactatgga agtttcgcca gaccttgagc cctcgcaggc attcatgcat
      541 gtgaacgacg ttctattcgc caatattcga cgattgaggg aaaggaaata catgactgcc
      601 gcgttttatc gcgcggatgc aaaagggaat ttctcttttg ccggtctcca tcaggatgca
      661 ttgatctaca gggccgccgg caagaagctc gaagtagttc cgacagatgg tttctggctg
      721 ggggtggacg ctggcgtcaa aaaggacatg cgcgcaggca agttcaagct gtctgtgggg
      781 gatctccttt tcttgtttac ggatggcctc gtagaatcta tgaatgggaa aaaagaaatg
      841 ttcggtcagg aaaaaataga ggagatcgtg cgtaagcacg gaacctcgga tctggccggc
      901 ctggaagagc gaatcatgaa cagcttgcgt gcgcatacag gggggctcag gccagccgac
      961 gatatcactt tcgccatgat tcggcgggag aagtaatatg tcattttcgc ttacaattga
     1021 accagaatgg aacgtgatca agaagatcaa agaatctctg caaacggatc ctgctctcaa
     1081 aggactcagc aaagatttcc tcgagcagac cacagtcacc gccattgagt tgcttgagaa
     1141 cgctttgaaa tacaccgact ctgattcagg acgacccgtt gaattctcat ttgattcgca
     1201 ggccggatat tgtgcctttc gcgtgaagaa ctatagtaag aacgcccaga acaaagacgc
     1261 tcttcgctcg gtcattgatc gtatccgcga cggagatcct tttgaattgt atgtagcacg
     1321 attggaacag ttgaaggaag atcccgacgg gcacagccgg atgggattgt atcgcatagc
     1381 gtacgaagga gagtaccatc tcagtgctga tattgacggc gaaagcgtga cgattctggc
     1441 ccgccggccg ctggagaatg cataatggct ctttcgaatt tgaccatgca ggacttcgaa
     1501 atcgaagcag tcgtcagcgg ttcgaatatc gaattgaact ggaaaggtgc catgcatgcc
     1561 cccaatccag aagatgtgct ggatccttat ttcgagcagg tgatcgcaga ggcacttaaa
     1621 gcaaagcttg gagtgaagtg caacttcgtg cctctggaat atatgaattc agcaagtatt
     1681 cctcctctga ttgctcttct tcgcactctg gcagacaaaa agattcaggg tgagttcgtg
     1741 tacgattcca atagaaaagt tcagtcagct tcctttcgtg cccttgatgt gattgcgcga
     1801 aagtcaaagt acacatctgt tcgagggatt tgatggtaga cgtccgggat ttatggcagg
     1861 agtcgccttc cgcggaactg gttctgcgag acggtaaact aatggccatg aacccggcgg
     1921 cagagaagct gctgggaatc actgcttccg atctgggtga cagcgtctgg gaagaaagag
     1981 tcttcgatga aaaagcaaga aagaagtttc gcagctttct tgaaagccag gctcccgtat
     2041 ccagcggcct tgatgcaagc ggcgcggacg gttcgattcg tcatattcgc tttttaaaaa
     2101 gaaacattcc ttcgcacgga actttgctca gcgttcagga cgtcactgaa acgcgggaag
     2161 atgcagaagc gttccaggcg ggctacgacg agttcatccg cgtcacaaca gatctagaag
     2221 acgcgcttgc gacgattgaa aagcagaaca gacagctcga aagacaaaag cagattctag
     2281 aaggagagct ccaggttgca cacgcagtgc aggcgcaaat cctttcgcag gacttcgaga
     2341 agcatactct tgtaaacgtt ttcggaacct accaggcaat gacagagctt ggcggggaca
     2401 tgtgggaatt ttttgagtca gagaacggca cgtgggtggt gattgctgat gtgatgggac
     2461 acggagttgc ttccagtctc atctccattg ctgcgaagtc aattttcaaa agaagattcg
     2521 aggaagccag ttctggcaaa atggatctgg cggagcttgt gaaagctgcg aatcgcgatt
     2581 tgtgcgaact cactcattcc aattactttc tgactgcatg cgccgttcat attgattcaa
     2641 atcatcgtct ggaatactta actgcagggc atcctccggt tctgtacgtt cccgccgatt
     2701 cgtccagaca ggtggaactt ctttttacag agcagcccat gctgggtgtg tttcgcaaca
     2761 tcgaatatca ctcagtgcca tttcaaatgt ttgaaggaga tcgaatcttc atctacacgg
     2821 attgtctcat tgaaagcgtc aatgcagaag gagacgtgat cgatttgctt cagatcgcag
     2881 acaggcttcg tttgaaaccg ggcactactc ctcagacagt agtgcagggt atgctggaat
     2941 atcgccgcga atttgcaggg tcagatcgcc ttccagacga tttgaccatg gtgtgcattg
     3001 agattcctca ggcggccggg ctgcgggtgg caaaatgatt cgcgtctatg catgcattct
     3061 aactgccgcg atgttgtgcg cggccgggtt gattgcggag cgcaggcaca ttgcagtcat
     3121 cgtatcttct gacactggca tctatgcgga tgcactgaca ggtttctatt cttcgtttga
     3181 tccggggctt gcacaaattg atcaaccagt gtatgtggat gcccttccag atccagcgcg
     3241 gtttttttca gaaagatcag atgcagatct cattgtgacc atcggatcca aggcaacgca
     3301 gcttgcgctt gcggaaggcc acggaagcaa gatcgtcttt tccatggtat cgagtccgcg
     3361 cgcggtcggt gttgcttcca ggcccgacgt gtgcggcaca agtatggacg tatccctttc
     3421 tgaattcttc aagtctcttc gcgagctttc gccttccgca cgtaacgtgt atgcatttta
     3481 ttccacgcct gaaggcgaat ttgcagcctc tgaaggggag tatctggatt tgcagttggg
     3541 cgttgaatat cacaagcgca gactttctgg caaatcggag cttccgcgag ctctcgaaag
     3601 tatccagggg aaagcggacg ctttttatct agtgccagat gccctttatg acagcgaaca
     3661 atttcagatt gtttctgact tctgcaagaa gaacaagatt gtgctgatga cttcgttctc
     3721 atctcttatg caagcaggtg cgacgttctc attgcgtcca gactacactc gcatcgggcg
     3781 tgtgacggca gagatggcag gtactgttct tcagggaaaa ggatgtgggc cgcgcagagt
     3841 cgttttcccg gatcaggcgt atttctcgtt aaacgagcgt tatgcggaag agtccggtct
     3901 gacgattccg caatcagtca aagagactgc cagtcgcact agattgtttc tatcagccgt
     3961 caacttgatg aatgagggta aaccaaagtc ggcactgatt gtttttgatg ccatcttgaa
     4021 gaaagcgccc gatgacgcgg ccgcgaaacg ttacagggac gccattgtag aaaggcagag
     4081 tggcgcgcaa accaggcaaa tgatggaatc
//
LOCUS       DAOQJL010000354         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13292_length_4102_cov_4.873487, whole genome shotgun sequence.
ACCESSION   DAOQJL010000354 DAOQJL010000000
VERSION     DAOQJL010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13292_length_4102_cov_4.873487"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..2082)
                     /locus_tag="PLG78_13990"
     CDS             complement(7..2082)
                     /locus_tag="PLG78_13990"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24280.1"
                     /translation="MKGILVAICLFCAHSVFGREIALGTDEHLMAGGEAEELRGPFTL
                     DEARVSTSWKDLNGKAPNYGFTREVIWLRLQIHSDEQTERILYLDGWADHLDIYTVHD
                     GVTVYHDAGLKARLTEEGKRFRRIFLPLVFDRGTTQIFFRMESSDTMRFPLEIFTRAH
                     FERFRDTSIALISLYFGGMIVMAIYNLFLFAVTRDKSYSIYVLFVTSSLLYFAAQSGF
                     ILQFFPFVGEVALKRLNLIGSSLVALTMTSFASIFLGLKIGFPRMALIMNVIRVASAA
                     LLVCSFFPAVPYGVCAVLISYVALAGLAMGVISGLVVWKSGYSPARYYLLAFTTLVAG
                     AVVFILGIQGVIGHNILTAHSMQIGSAAEVTLFAIALSDRISLLNRTVDRQMKELESA
                     HALLANSEKKYRTIVEDTTDVIFSLDAEGRIGTINQAARKMLGYWPSMLEGTLFESLI
                     FQRDGISLELGIALFRQSLLTLGEEPFQLRLPLRSRSGEPVDFQVRVEKIGEGSHAFI
                     LRKASAAGEDVLLHFLEIERGSYIIDNYLSTSELLSHRITRNLTRFFDDQAVADIQLV
                     LREMLLNAIEHGNLEVDFDAKTAAQRDGDYLKFLQERQNRSEFRGRRVRVDFGLNQDR
                     VWYRIADEGKGFDHANALRQAQKNLRDTQSTHGRGIGIASRLFDIMQYNAKGNQVFLV
                     KNVHSNQVS"
     gene            2206..3786
                     /locus_tag="PLG78_13995"
     CDS             2206..3786
                     /locus_tag="PLG78_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24281.1"
                     /translation="MKKFILLTLVFTVAPVSAQDFYRQGKIAYGQKNYERARQLFEQA
                     MQANPSNGNPCFYLGYMLDNQNRRDLAVQQYRRGVDLQMDADLREKSFWKIVLYYKYV
                     QDWENLAIYSEKFLHYRDIPQVKEFLEEAKERRDPAEGKLRELTASAEKKRAAGDNKG
                     AAADYRSILAIKWDEHSAWNLAALLMQMKEYSEALTMYNRLIDQKPGWEYFYKRGACH
                     YSLRNYDASLQDFNKARTLNKKPDAGFKHFTALGEGLTLIELARYEEAREKLKQSLAL
                     KKSATAAAALSRLEWIHGNRSESERYAALASENEREKNHLTALMLFQSEKKPEERKNG
                     AAAFSKWLASLSDEKYLHRTWTADLFILGRQGCIQANQPLCIPALEKIPEADRFHLGN
                     LVTSAYPTEKRNAADVQRDFDFYYGKGLLDEGKVDAGLSALSRVSDTPVGAYYMAVGF
                     AKKSQEKNAMDYLQRAAEKKPEYWDYAGKNEHFKALAARSSDFAYFLEHKGKPRPVEP
                     AVIPGNEVKNEKAKDTSP"
     gene            3758..>4102
                     /locus_tag="PLG78_14000"
     CDS             3758..>4102
                     /locus_tag="PLG78_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24282.1"
                     /translation="MKKQRTLLHDPALYDPYRGLYTRAELEKVRSYLKEELSRSLKHF
                     KKNTSPRPHFISYLFRNFRSEKIAGRLGGITDHTTGSQNTTYCDVRVGGPKFDNVING
                     GLEDNSDRDESIE"
BASE COUNT         1155 a         1054 c         1013 g          880 t
ORIGIN      
        1 ctaacgtcag ctcacttgat tggagtgaac gttcttcacg aggaacacct gattcccttt
       61 cgcgttgtat tgcattatat caaagagtcg acttgcgatt ccgattcccc ggccgtgagt
      121 actctgcgtg tcgcgcaagt ttttctgtgc ttgcctgagc gcattcgcgt gatcgaaacc
      181 cttcccttcg tccgcgatgc ggtaccacac cctgtcctga ttcagaccaa agtccacgcg
      241 aacacgcctt cccctgaatt ccgaacggtt ctgtctttcc tgtagaaatt ttaagtaatc
      301 gccgtccctc tgcgccgccg tcttcgcgtc aaagtccact tccaggttgc cgtgttcaat
      361 ggcgttcaac aacatttcgc gcaacacaag ctggatatcg gcaacggctt gatcatcaaa
      421 gaaacgagtc aggtttcgcg taatccggtg actcagcaat tccgatgtac tcaaatagtt
      481 atcaattata taggatccac gttcgatctc gaggaaatgc aaaagcacat cctccccggc
      541 cgcgctcgct ttcctcagaa tgaatgcatg ggatccctct ccgatcttct caaccctcac
      601 ctgaaaatcg acaggctcgc ccgaccgaga gcgtaaagga agacgaagct gaaaaggctc
      661 ttcgccgaga gtgagcaacg actgcctgaa cagggctatg cccaattcca gagaaatgcc
      721 gtcacgctgg aagatcaaag actcaaaaag ggttccttcc agcattgaag gccagtagcc
      781 gagcatcttc ctggcagcct ggttaatggt gccaattctt ccttctgcgt ccaaagaaaa
      841 gataacatct gtcgtatctt ctacaatagt acggtatttc ttctccgagt tggcaagaag
      901 cgcgtgggcc gactcgagtt ctttcatctg tctatctact gtgcgattca ataggctaat
      961 tcgatcagac agagcaatgg cgaataacgt aacttccgct gcggaaccaa tctgcatact
     1021 gtgagccgtc aagatgttgt gcccgatcac gccctgaatg cctaaaataa aaacaactgc
     1081 tccggccaca agtgttgtga acgcaagaag gtagtagcga gcgggactat acccgctctt
     1141 ccagactaca agtccagata tgactcccat ggccaggcct gcgagcgcaa cataggaaat
     1201 gagcacagcg caaacgccat acggcacggc cgggaaaaag gagcacacaa gcagagcggc
     1261 agacgcaaca cggatcacgt tcatgatcaa agccattctt ggaaaaccaa tcttcagccc
     1321 cagaaagata gaggcgaagc tggtcatggt tagcgcaacc aggctcgatc caatcaggtt
     1381 cagtctcttc agggccactt cgcctacaaa gggaaagaac tgaagaatga agccggattg
     1441 agctgcaaag tagaggagcg aggaagtaac aaacaatacg taaatggaat acgatttatc
     1501 ccgcgtcact gcgaagagaa aaagattgta gatcgccatg acgatcatcc cgccaaaata
     1561 aaggctgatc agagcaatcg atgtatcgcg aaagcgttca aagtgtgcgc gagtaaaaat
     1621 ctcgagcggg aaacgcatgg tatccgaact ttccatgcga aagaagattt gggttgtgcc
     1681 cctgtcaaag acaagaggaa gaaaaatcct gcgaaagcgt ttcccttctt ccgtgagtcg
     1741 agctttcaga cctgcatcat gatagaccgt cacgccgtcg tgaacggtat aaatatccag
     1801 atggtccgcc catccgtcga ggtagagaat gcgttccgtt tgttcatcgg aatgaatctg
     1861 tagacgaagc cagattacct cccgggtaaa gccgtagttg ggcgcctttc cgttcaggtc
     1921 tttccaggac gtagatacgc gtgcttcatc aagagtgaag ggaccgcgca gctcctccgc
     1981 ttccccgccg gccatcaaat gctcgtcggt gcccagggca atttcgcgcc cgaaaaccga
     2041 gtgcgcgcaa aacaggcaga tcgcgacgag aatgcctttc atcgatccca gcggggaatc
     2101 cggggctcac gcatgcaagc gatttaaccg gcaacctgtc cgaatgcgag gcagctcaga
     2161 aatcattgcg cgcaacagtc aatcgtgcgt tctgtcctca ggaatatgaa gaagttcatc
     2221 cttctgactc ttgttttcac cgtagcacca gtttctgccc aggatttcta tcgccaggga
     2281 aagattgcgt acggccagaa gaactacgaa agggccagac aacttttcga acaggccatg
     2341 caggccaatc catccaatgg caatccttgc ttctatctgg gttacatgct cgacaatcag
     2401 aaccgcagag acctggcagt gcaacaatat cgccggggag tagatctgca gatggatgca
     2461 gatcttcgcg aaaaatcatt ctggaagatt gtgctctact acaagtatgt tcaggactgg
     2521 gaaaaccttg ccatctactc agaaaaattt ctgcactatc gcgacattcc tcaagtaaag
     2581 gaattcctgg aagaggcaaa agagagacgc gatccagcgg aaggcaaatt gcgcgagtta
     2641 accgcaagcg cagaaaagaa gcgagcagcg ggcgacaaca aaggtgcggc ggcggactac
     2701 agatccatcc ttgcaatcaa atgggacgaa cactctgcat ggaacctggc agcactgctc
     2761 atgcaaatga aagagtacag tgaagcgctc acaatgtaca atcgattgat tgatcagaag
     2821 ccaggctggg aatatttcta caaacgtggg gcctgccact acagccttcg caactatgac
     2881 gcctcactgc aggatttcaa caaggccagg acattgaata agaaaccgga tgcgggcttc
     2941 aaacatttca ccgctctagg agaggggttg actctcattg aactggctcg ctatgaagag
     3001 gcccgcgaaa agctgaagca atctcttgcg ctcaagaaat ccgctacagc agcagccgct
     3061 ctgagtagac ttgaatggat tcatggcaat cgaagtgaat ctgaacgtta tgcggcattg
     3121 gcgtctgaaa atgaacggga aaagaatcac ctaactgcgc tgatgctctt ccagagtgaa
     3181 aagaaaccgg aagagagaaa aaatggtgct gctgctttct cgaaatggtt agcctctctg
     3241 tctgatgaaa aatatctcca ccggacctgg actgcggatc ttttcatact gggccgtcag
     3301 ggctgcatcc aggcaaacca gccactctgt atcccggccc tggaaaaaat tccagaggca
     3361 gatcgcttcc atctcggaaa tctagtcact agtgcttacc cgaccgaaaa gagaaatgca
     3421 gcggacgttc aaagggattt tgacttttat tacggaaaag ggctgctgga tgaagggaaa
     3481 gtggacgcag ggttgtccgc actctccaga gtgagcgata cacctgtcgg cgcctactac
     3541 atggcagtcg gttttgcgaa gaagtctcaa gaaaaaaatg caatggatta tttacaaagg
     3601 gcggcagaga aaaaacctga gtactgggac tatgctggga agaatgagca tttcaaagcg
     3661 ctggcagcca gaagctctga cttcgcttat tttctggagc acaaggggaa gccacggcca
     3721 gtggaacctg cggtgatccc tggcaatgag gtcaagaatg aaaaagcaaa ggacacttct
     3781 ccatgacccg gccctgtatg atccctaccg tggtctatac actcgcgcag agctagagaa
     3841 agtaagaagc tacctcaaag aagagctgtc acgttctctc aagcatttca aaaaaaatac
     3901 ttctcctcgc cctcacttca tttcctatct cttccgcaat tttagatcgg aaaagatcgc
     3961 gggacgcctg ggcggcatca cagatcacac aaccggttca caaaacacca cgtactgcga
     4021 cgtgcgagtg ggcggcccaa agttcgacaa cgtaatcaat ggtggtctag aagataattc
     4081 tgacagagac gaatcgatcg aa
//
LOCUS       DAOQJL010000355         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13384_length_4080_cov_4.370435, whole genome shotgun sequence.
ACCESSION   DAOQJL010000355 DAOQJL010000000
VERSION     DAOQJL010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13384_length_4080_cov_4.370435"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLG78_14005"
     CDS             complement(<1..517)
                     /locus_tag="PLG78_14005"
                     /inference="COORDINATES: protein motif:HMM:NF020035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24283.1"
                     /translation="MLTKLDLSQCEKEAIHIPNLVQPHGFALVIEKSSLNVTRSSANF
                     DSTSIGPASQLVGRSLQSILPPAALAAISQKLDFSQRKRHVLYGLAIPSISVSSLNVA
                     VCDAHREIILEFLPGEADGGEEFVELQLNDMIPRVMMAQSSEDFFQRAAIELRSLTGY
                     DRVMVYRFDEDF"
     gene            complement(486..1094)
                     /locus_tag="PLG78_14010"
     CDS             complement(486..1094)
                     /locus_tag="PLG78_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNE24284.1"
                     /translation="MVLEKGLRERLKRLTRPFHREVETAFYPARLLSGALTAPQYRDY
                     LQTLHSVHVAWENSWASFSEWSLLGIPRIEQRFRASHAREDLSILGASPLVTSVAWSP
                     STFAHAIGSAYVLEGSTLGGAILGKKIPQIFEGKPEAACVNYFAGRGESAVPLWAEFC
                     GLLDRVDQERPDLVPSIISGACMAFLSMEKELDVDKTGSISM"
     gene            1130..1291
                     /locus_tag="PLG78_14015"
     CDS             1130..1291
                     /locus_tag="PLG78_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24285.1"
                     /translation="MLGRLKNPLGDLSALPGARSMFFLRLNLAKAWTKIILDQGISVR
                     TPKILRVTA"
     gene            complement(1281..1952)
                     /locus_tag="PLG78_14020"
     CDS             complement(1281..1952)
                     /locus_tag="PLG78_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24286.1"
                     /translation="MKLIHTIAILMSLLLTACNSDNGSSNDSSLLLLGLAAATRTASS
                     TTAQTCAQSAANPVSSVSEIGGVCATPTLVQVGDGTVRDTANNILWIQCAMDDDGSPG
                     AVHETSPMACQTIGASQVFTASIGQGLCASLTYAGRTGWKLPDMRQLGAHFKNQAAAP
                     HVVDSALTVLTLSDVTAYNYVTTTTGTGSQVGMVEFRNNSGSGGGMKFAASGTTGIVT
                     CMIKL"
     gene            complement(2062..2682)
                     /locus_tag="PLG78_14025"
     CDS             complement(2062..2682)
                     /locus_tag="PLG78_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE24287.1"
                     /translation="MKRRILIVDDHPVVLAGIQRALMDRKMEVHCTRSLKDARSILGS
                     AETFDLVLLDIHLEDGLAFDLALELRKSARDLLVAFLTSSRDWNHLVKARQTGARGFL
                     LKDAELDSIAESIERILSGETVFPGIESPQSISAETAAAVHSLTEREREVMRYTSQGL
                     LNREIADRMNVSLRTVESHRSKAAEKLGARGTIQLASILAELKHLL"
     gene            complement(2714..>4080)
                     /locus_tag="PLG78_14030"
     CDS             complement(2714..>4080)
                     /locus_tag="PLG78_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE24288.1"
                     /translation="AVFGPCAAAEAFLPVRWFTHLLPVHNLFLLLFSLYFLVIAFQAV
                     RERMPGAAAIWFGWLVAISANVNDSLYIMSAIDSAYLSAIGLQAFILTQSYYLARRYS
                     EAFALSAELARRMQSLLGLTREFNRAADRGTAVRRALREISDILEKPDLQAFIPVRDS
                     PLMRKISADRPDEFADLPWSTKAESITSQVQEDGRVLIPLRGGGELLCLIDLPGFEDA
                     RTQYVQGILDSLSLVLDNMRRVESVALASIGQAAAEIVHDINHHCQVIKAHASSGQHS
                     VERLRLIENETGLMTNLALDILDFARENVIVNPMAVSLESVKDAFNQDIGSAFMNTDM
                     RHSIVVNAAGDIRIDLERFRRVCLNLARNAMEACNGSGLFRVEIEIGSDTVIMVFEDD
                     GPGIDPAMQEKLFEPFASGGRGTGLGLAVVRRIVSAHGGDIRVYSTPGKGCRFVVMVP
                     LNFG"
BASE COUNT         1051 a         1127 c          980 g          922 t
ORIGIN      
        1 tgaaatcttc atcgaacctg tagaccatga ctcgatcata accggtcagt gaacgcaatt
       61 caatggccgc tctctgaaaa aaatcctccg aactctgcgc catcatcacg cgcggaatca
      121 tatcattgag ctgaagctct acgaactctt cccctccgtc tgcttctcct ggcaagaatt
      181 ccagaatgat ttcgcgatgc gcgtcgcaga ccgcgacgtt cagactggag acagaaatgg
      241 aaggaatcgc aaggccatag agaacgtgcc tttttctctg cgaaaaatct agtttttgcg
      301 aaatcgcagc cagcgctgct ggcggcaaaa tactctgcaa gcttctgccc acaagctggg
      361 acgcaggacc aatggatgta gaatcgaaat tcgcgctcga acgcgtgacg ttcaggctgg
      421 acttttcgat gaccagcgcg aacccgtgtg gctgcacgag attcgggatg tgaatggctt
      481 ctttttcaca ttgagataga tccagttttg tcaacatcga gctctttctc cattgaaaga
      541 aatgccatac acgcacctga tattatggat gggacaagat ccggtctctc ttgatccacg
      601 cgatccagca atccgcagaa ctccgcccat aagggtacag ccgattctcc tcgccccgca
      661 aagtaattca cacaggctgc ttcaggcttg ccctcgaaga tctgagggat tttttttcct
      721 agaatcgcac cacccagggt ggaaccttca agcacgtagg cggaaccgat cgcatgtgca
      781 aatgtagacg gactccaggc aacggaagtg accagagggc ttgcacctag aatcgacaag
      841 tcctctctgg catgactggc gcgaaaccgc tgttctatac gcgggatgcc gagtagtgac
      901 cattctgaaa aagaggccca ggaattctcc cacgcaacat ggacggaatg aagcgtctgc
      961 aaatagtcac ggtactgtgg cgccgtcaat gctccagaaa gaagtcttgc tggatagaaa
     1021 gcagtttcta cttcgcgatg aaacgggcga gtgagtctct ttagacgttc gcgaagtccc
     1081 ttctccaata ccatgattcg accgccgcgt tccagtaatc gaaaacagga tgctcggtcg
     1141 actcaaaaac ccgttaggcg atctcagcgc cctccccggc gcacgttcca tgttctttct
     1201 gagactcaat ctagcaaaag cctggacaaa aatcatcctc gaccagggaa tttcggtgcg
     1261 aacgccaaaa atccttcgcg ttacagcttg atcatacagg ttacaatacc cgtggttccg
     1321 gatgccgcga acttcattcc accgccagaa ccagagttgt tcctaaactc aaccatgcca
     1381 acctgactac ccgttccagt tgtggttgtg acatagttgt aagcagtcac gtcactcaga
     1441 gtgagcactg ttagcgccga atccactacg tgaggcgcag ccgcttgatt cttaaaatgg
     1501 gcaccgagtt gacgcatgtc aggcaacttc cagccggtgc ggcccgcata cgtgagactc
     1561 gcacagaggc cttgacctat ggacgctgta aacacctgcg aagcgccaat ggtctggcaa
     1621 gccatcggac ttgtttcatg tacggctccc ggagatccgt cgtcatccat cgcgcactga
     1681 atccagagaa tgttgttcgc agtatcgcgc accgtgccat cccccacctg cacgagcgtg
     1741 ggcgtcgcac agactccgcc gatttcagaa acactggaca cagggttagc cgcgctttgt
     1801 gcgcaagtct gagcggtggt gctcgacgca gtcctggttg cggccgcaag gccgagaagc
     1861 aacagagacg aatcgttgct cgatccattg tctgagttgc aagcagtgag caaaagggac
     1921 atcaagatcg caatggtgtg aatcagtttc atgttcgtat ctccgtgcaa aggatcgcac
     1981 ggaaacacgc ctttgtcagc agaaggaacc gcaaagatga tctaaaaaat gcgggttcta
     2041 ccaggaatct ttaaagaaat ttcaaagaag gtgcttgagc tcggccagaa tggaagccag
     2101 ctggatggtg cccctggcgc cgagcttttc tgctgctttt gaccgatgcg attcaacggt
     2161 gcgcagactt acgttcatgc gatctgcaat ttcacgattc aagagtccct gcgatgtata
     2221 tcgcattact tctcgttctc tttccgtcag actgtgaact gctgccgcag tttccgcaga
     2281 tatgctctga ggagactcta ttcctggaaa gaccgtctcc ccggagagga tgcgttcaat
     2341 gctttctgca atcgaatcaa gttccgcatc tttgagaaga aaaccgcgcg cgccagtctg
     2401 cctggctttc acgagatgat tccagtcgcg cgaagaagtc aaaaacgcaa ccagcaagtc
     2461 gcgagctgat tttctcagct ccaatgccag atcaaacgca agaccatctt ctagatgaat
     2521 gtcgagcaac actagatcaa atgtttctgc gcttcccagg attgatcggg catctttcaa
     2581 agaccgcgtg cagtgcactt ccattttccg atccatcaat gcgcgctgga taccggcgag
     2641 tacaactgga tgatcatcta caataagaat tcttcttttc aactctttct ggctccccgc
     2701 tttctgtacc tggtcaaccg aaattcaaag gaaccatcac tacaaaacga catccttttc
     2761 cgggagtcga ataaactcga atgtcaccac cgtgcgcgga aacaatcctc ctgaccactg
     2821 ccaggccaag ccctgtcccg cggccgccac ttgcaaacgg ttcaaacaac ttctcttgca
     2881 tggcaggatc aattcctggg ccatcgtctt cgaaaaccat gatcactgta tccgagccga
     2941 tttcgatttc aacgcgaaac aatccggatc cattgcatgc ctccattgca tttcgtgcaa
     3001 gattcagaca cactcttcgg aatcgctcca ggtcaatgcg aatgtctcct gcagcgttca
     3061 ctacaatcga atgccgcata tcggtattca tgaaggcaga tccaatgtcc tgattgaacg
     3121 catcctttac actttccagt gacacggcca taggatttac gatcacattc tctctggcga
     3181 agtctagaat gtccagggcg aggttcgtca taagcccggt ttcattttca atcaaacgaa
     3241 gcctttccac tgaatgctgg ccagaggaag cgtgcgcctt gatcacctga cagtgatggt
     3301 tgatgtcatg aacgatttca gcggccgcct gtccaatcga ggcgagcgct acgctttcca
     3361 ctcttcgcat gttatctaga acgagagaca gagaatcaag aatcccctgc acgtattgcg
     3421 ttctagcgtc ctcaaacccc ggcaaatcaa tcaagcacag tagctctcct ccgccacgca
     3481 agggaatgag cacgcgaccg tcttcctgaa cctgtgatgt gatggattcc gcctttgtgg
     3541 accaggggag atcagcgaat tcatccggtc tgtccgctga gatctttcgc atcagaggtg
     3601 aatctctcac aggaatgaac gcctgaaggt ctggcttttc cagaatgtcg ctgatctctc
     3661 gcaacgcccg acgaacagct gttccacgat ccgccgcgcg gttgaattcc cgggtcaagc
     3721 ccagaagact ttgcatacgc ctggccaact ccgcactgag cgcgaaggct tcagaatatc
     3781 gtcgtgccag atagtaggac tgagtaagta tgaacgcttg taggccaatg gcagaaagat
     3841 aagcagaatc aattgcgctc atgatataga gagaatcgtt cacgttagcc gaaattgcga
     3901 ccaaccatcc gaaccagata gcagccgccc cgggcattcg ctccctcaca gcctgaaagg
     3961 cgatgaccag aaaataaaga ctgaacaata gcaggaataa attgtgaaca ggtaagagat
     4021 gagtaaacca tcgcaccggg agaaacgcct cggcggcagc acagggtcca aaaacagcaa
//
LOCUS       DAOQJL010000356         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13388_length_4079_cov_4.153827, whole genome shotgun sequence.
ACCESSION   DAOQJL010000356 DAOQJL010000000
VERSION     DAOQJL010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13388_length_4079_cov_4.153827"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="dnaX"
                     /locus_tag="PLG78_14035"
     CDS             complement(<1..942)
                     /gene="dnaX"
                     /locus_tag="PLG78_14035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNE24289.1"
                     /translation="MTEVAHLVLARKYRPRVFKEVVEQGLAVTALKNAADHGRIGSAY
                     LFSGARGTGKTTIARILAKRVNCLNPKDSEPCCECESCVSIQNGSSLDVQEIDAASNR
                     SIDNIRDLRENVKFQPMNAKRKVYIIDEVHMLTPEAFNALLKTLEEPPEHVLFVLATT
                     ELNKIPETILSRCQTFAFRKVPLNILQNHLKDICNREKIKAEDEALFWIARRGDGSVR
                     DSLSFLEQSITYTEGNVTTGKVKELIGALPLDLYLGLIQMLLDPKTKFPDLVEPVHRR
                     FDEGADLRRFIWDFLDLLRALSHVLHGVERPEFVGLPP"
     gene            1026..1580
                     /gene="pth"
                     /locus_tag="PLG78_14040"
     CDS             1026..1580
                     /gene="pth"
                     /locus_tag="PLG78_14040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE24290.1"
                     /translation="MADRFLIIGLGNPGKKYSKNRHNAGFLALDGVASAFGAGSFTKD
                     KKGEWALAQGPDKSEIYFYKPLEFMNLSGGGVKDFTKKHPVETARVVVLHDEIELPFG
                     EVRWKVGGGHKGHNGLRDIIARCGSPDFHRVRLGMGRPDHDDVAEYVLSDFMPEEFKS
                     FSLMTDRAKSLIEKWLVNPDKAPA"
     gene            complement(1583..3541)
                     /locus_tag="PLG78_14045"
     CDS             complement(1583..3541)
                     /locus_tag="PLG78_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24291.1"
                     /translation="MSRSAVELRGDWQFFPGSFLSNAASDVTPVYVKVPGSWNSYYPA
                     AAIGGGQGFGTYRIQIRTTDQDLALSTMEQGTSVRVYANGKLICEAGAPGSNRDTTIA
                     HAVPLLCDIGRVSNALTLTLEIANFNYRKGGMWNAPVIGPVNAVRRDFYLRRDLELML
                     SALLFVMSMYHVMLYVYRRVDLVPLVFGLFAFAVFLRTISTNFRILTQYAPFLPFEFY
                     NRLELLSWFWIPPLALEYTSRIFSGRIPSRTNLVFGVLAILESLFLVLPPALYSYVVF
                     PSQILSVAVLVLCIYIPWAAMREGHPGAAAFLFSSLVAVACVTNDLLYTNEVIRTAQV
                     GPYGLFFVIIAHSLILSHMLMRTYKLLVQSDSRYRIMIEGSRRMVFALDRQFRFTSAN
                     RTVSRTLGLSQEELVGRSFLDFIYEDASEHGNVRALVRQKLEDVAGSEKPAQFSIGLV
                     QVIGDGPIISLFTFEVVELADRWEMLGRAETDLSDSLLPRFVREHQRYAIGNSLTEAE
                     ELAHRLTRHLARYADLEKASYIRLGLREILINAIEHGNLEVSFDEKTETQMGGDYVSY
                     LQERCSLSQFKGRMVRVAYSLNTKRVIYRIEDEGKGFDFDALFARIHQSQPDSDLAHG
                     RGILMTLNIFDTVRFVAPGNVVFLVKKF"
     gene            3775..>4079
                     /locus_tag="PLG78_14050"
     CDS             3775..>4079
                     /locus_tag="PLG78_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24292.1"
                     /translation="MSRASIATILLICFASIFAAARVDKDQCFFMDSLAKYAQSRSLA
                     DQNFQNEEYYYPARNNMDPEFHFFPFGGIYLVKTPANQYLGQYPIAFTFLSAVVLKF"
BASE COUNT         1095 a         1034 c          987 g          963 t
ORIGIN      
        1 gggagggagg cccacaaact ctggtctttc cactccgtgc aagacgtgcg aaagcgcgcg
       61 aagtagatcc agaaagtccc agatgaatct ccgcaagtcc gcaccttcat caaacctgcg
      121 atgaaccggc tccaccagat caggaaattt ggttttagga tccagaagca tctggattaa
      181 accaagatag agatccagag gcagcgcccc aatcagctct ttcaccttac cggtagttac
      241 attgccttcc gtgtacgtaa tgctttgttc taaaaaagaa agactatcgc gaacagatcc
      301 atctccacgt cttgcaatcc agaaaagagc ttcgtcttcc gctttgatct tctcgcggtt
      361 gcatatgtct ttcaaatgat tctggagtat attcagaggt actttgcgaa atgcgaacgt
      421 ctggcatctg gataagatgg tttctggaat cttattcagt tccgttgtag ccaaaacaaa
      481 caaaacgtgt tcgggcggtt cctcaagagt tttcagcaga gcgttgaacg cctccggtgt
      541 gagcatgtgt acttcatcaa tgatgtagac tttacgcttc gcgttcattg gctgaaactt
      601 tacattctct ctgagatcgc gtatattgtc gatggagcga ttggacgcgg catcaatttc
      661 ctgaacatcc agagatgatc cgttctggat ggacacacag gattcacact cacagcaagg
      721 ttcgctgtct ttaggattca gacaattcac tctttttgca agtatcctgg caatcgtagt
      781 cttgccggtc ccgcgcgcgc cggagaacaa ataggcggaa ccaattctgc catgatccgc
      841 agcgttcttc aaagcagtaa ctgcgagccc ctgctctaca acttctttga atactctggg
      901 ccgatacttg cgcgcgagca caagatgcgc gacttctgtc atgatggatc aaggtaccaa
      961 tgctgtttcg cagaacaaca ttattttctg attgtgcccg atcgcgcaaa acgcacggag
     1021 gttgcgtggc agatcgcttc ttaatcattg gccttggcaa tcccgggaag aaatattcaa
     1081 aaaatcgaca caatgccgga tttctggccc tggatggcgt agcatccgcc tttggcgcag
     1141 gctcgtttac gaaagataaa aaaggtgaat gggcactcgc ccagggcccg gacaagtctg
     1201 agatttactt ttacaaacct cttgaattca tgaatctttc tggcggtggc gtgaaagatt
     1261 tcacaaaaaa gcatcctgtg gagactgcca gagtggttgt actccatgac gaaatcgaac
     1321 ttccatttgg cgaagtacgg tggaaagtag gaggtggcca taaaggacac aatggtttgc
     1381 gcgacatcat tgcgcgttgc ggaagtccgg actttcaccg cgtaagactt ggaatgggcc
     1441 gcccggatca cgatgatgta gctgagtatg ttctatccga tttcatgccg gaagagttca
     1501 aatccttttc tcttatgaca gacagagcaa agagcctgat tgaaaagtgg ctggtcaatc
     1561 cagacaaagc gccggcctga aactaaaatt tcttaactaa aaacacgaca ttgcctggag
     1621 caacaaagcg aaccgtgtca aaaatattca gagtcatcag gattcctctg ccatgcgcca
     1681 gatcgctgtc cggttgactc tgatggatcc tcgcaaatag cgcgtcaaaa tcaaatccct
     1741 tgccctcatc ctcaatgcga taaatgactc gtttggtatt caaggaatag gccactctca
     1801 ccatgcgtcc cttaaattga gaaagactgc atctctcctg gaggtaggaa acatagtctc
     1861 cccccatttg cgtttccgtc ttctcatcga atgatacttc tagatttccg tgctcaatcg
     1921 cgttgatcag aatttcgcgc aggcccaggc gaatgtagga tgccttttcc aggtctgcgt
     1981 atcgcgcgag atgtcgagtg agacgatgcg cgagttcttc tgcctccgtc aggctattgc
     2041 cgatcgcata acgttggtgc tcacgcacaa aacgaggcag caggctgtct gaaagatctg
     2101 tttctgcacg cccgagcatc tcccatctgt ccgcgagctc tacaacttcg aaggtaaaca
     2161 gcgagatgat tggaccatct ccaatcacct gcaccagacc aatggagaat tgcgctggct
     2221 tttcggatcc tgcaacatct tctagcttct gtcgcacgag cgcgcgaacg tttccatgct
     2281 cggacgcatc ttcgtagatg aagtccagaa agcttctgcc tacgagttct tcctgactca
     2341 ggccaagcgt acgggagacg gtgcgattcg cagacgtaaa tctgaattgt ctgtccagag
     2401 caaaaaccat gcggcgcgag ccttcaatca tgatgcgata gcgactatct gattgaacta
     2461 acaacttgta ggttcgcatg agcatgtgag acagaatcaa agaatgcgcg ataatcacaa
     2521 agaacaggcc atacggccca acctgtgccg ttcgaatgac ttcattcgtg tagagcaagt
     2581 cattcgttac gcaggcaacg gcaaccaggc ttgaaaacag aaaagcggcc gcccctggat
     2641 gtccttcgcg catcgccgcc cacgggatat aaatacatag aaccagaacc gcaacggaca
     2701 gtatttgcga aggaaatact acgtagctat agagcgccgg tggcagaacc aggaagagag
     2761 actcaagaat agcaagaaca ccaaagacca aattggttcg ggacggaatt cttcccgaga
     2821 aaatgcgaga ggtgtattcc agggcaaggg gcggaatcca gaaccaggag agaagctcta
     2881 gcctgttata aaattcgaaa gggaggaagg gagcgtactg agtcagaatt ctaaagttcg
     2941 tagaaatcgt gcgtagaaac acggcgaaag cgaaaagtcc gaacacgaga ggcacaagat
     3001 ccacacgacg ataaacatac aacatcacgt gatacatgct cattacgaat agcaatgcac
     3061 tgagcatcag ttcgaggtct cgccgcagat aaaaatcacg acgcactgca tttaccggac
     3121 caataacagg cgcgttccac attccacctt tgcggtagtt aaagtttgca atttccagag
     3181 tcagagtgag ggcattggag acacgaccaa tgtcgcacaa caaaggaact gcgtgagcga
     3241 ttgttgtgtc gcgattgctt ccgggagcgc ctgcctcgca aatcaattta ccattggcat
     3301 aaacccgcac tgaggttccc tgttccattg tggaaagcgc gagatcctga tccgttgtgc
     3361 ggatttgtat tctatacgtg ccaaatcctt gtcccccacc gatcgccgcg gcagggtagt
     3421 acgaattcca ggacccgggt accttgacgt agaccggagt cacatccgaa gcagcgttgg
     3481 acagaaagct tcccggaaaa aattgccagt ccccgcgaag ctcgaccgcc gatcgggaca
     3541 cggaagtgag atcaatcgat gcgccggaca ctaaaccgcg aacgcgacta tcgcactccg
     3601 taagacaaac gaaacttgca aaaagtagaa caagtctggc gagagtgcca cctggccgca
     3661 tgtgtctcaa actctgtcgc gcgttgcctg tgacaacatt ttatgcgaaa tcaagcaagg
     3721 acggacgact ggctttttta attgccatct tctccgcgaa acaaagcctg aaccatgtct
     3781 cgagcgtcca ttgctacaat cctgctgatt tgttttgcgt cgattttcgc ggccgctcgc
     3841 gtggacaaag atcagtgctt cttcatggac agtctggcca agtatgctca atcccggtct
     3901 ctggctgatc agaacttcca gaatgaggaa tactactatc cggcacgaaa caacatggat
     3961 cctgagtttc atttctttcc gtttggcggg atttacctgg taaaaactcc tgcaaatcag
     4021 tatctgggac agtaccccat cgcattcaca ttcctgtctg ccgtcgtttt gaaattctt
//
LOCUS       DAOQJL010000357         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13409_length_4074_cov_4.411296, whole genome shotgun sequence.
ACCESSION   DAOQJL010000357 DAOQJL010000000
VERSION     DAOQJL010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_4074_cov_4.411296"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1387
                     /locus_tag="PLG78_14055"
     CDS             437..1387
                     /locus_tag="PLG78_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24293.1"
                     /translation="MTKRILSILCLLVFPAAVFAQAPAQPAQPAQPATTPATTQPTDV
                     ANPANPTPYEPSPGELVTEEVNEAELSPEQKEVRDRNLKAEELWKNADYRDYNRAFAE
                     LHQLSKAFANNKYRLSLSAYQSGVNTIIKMRDEVELFRKESAETKRLNEKWYWQIVDR
                     KAKEERLLSNMKRTAKLEAVTYFTRAINHLDEIKNPDLREKPAFKKLLAAVYRNWIMY
                     QFDLGNLPQTIPILELYIEIDQNEKEYPSHKYLAQAYSFQENMIKKYGVGTEDQMFRF
                     RYKKNVHLLRATELKYGKESAEYKHVVNLVNRDEIISVQP"
     gene            1734..2453
                     /locus_tag="PLG78_14060"
     CDS             1734..2453
                     /locus_tag="PLG78_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24294.1"
                     /translation="MPEYPFNPANVSAMQFWLAADDVVPSTGTVVSRWPDRSANANHV
                     VQSTAAMQPTYTTGAAPYFHPVVYFDGGDQLTGLNTALTGADFTFFVVYNRPVAGGHP
                     FLEVGQVTLTNAKSLGVNGSLGVDVCSRNVACAPGPAGVTYTNNAWHAVTVTYSGSGN
                     QKQTQWRDGASVGSGTAQALAFDTVGFASVGNAPNGGAGFVGYIAEIIYYNRILSSSE
                     IDYVNCYMTLKFAIGSALFCK"
     gene            complement(2469..3692)
                     /locus_tag="PLG78_14065"
     CDS             complement(2469..3692)
                     /locus_tag="PLG78_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24295.1"
                     /translation="MRCPVRRTLCAIGFVLFTLSGTGCSQHQQCGDTDVSCKLMANLI
                     MYDICRVPYYKISDPLFRGSMMLEYNYQASKGSTGNPTPVQYGTLGGANDFYGSGGLL
                     APNGKIYAPPNDQNAINNQALIIDPKTNITSTIAGIGGAGPKWSPGALAANGKMYFFP
                     ISGTTSILSIDPTTNALSTFGSVTAGYGGAVLAPNGKIYAIPMTAGLPVAVFDPSTNQ
                     FTSISGATTGGWIGGVLALTGKIYAFPMGNIAAVGFLEIDPTNDSVSTFASFAGTQGF
                     FGGGMAPDGRFYSLPVGNHQSMVFDPFARTVSYFGTTYPLNQFEGNVVGPNGITYGSP
                     RGSATIDTLSMGPVVATGISAGGATAQTSGILVPTGRIYTIPRSASVPFQYLQTNARG
                     TLCENILMSPFFNKF"
     gene            complement(3761..>4074)
                     /locus_tag="PLG78_14070"
     CDS             complement(3761..>4074)
                     /locus_tag="PLG78_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE24296.1"
                     /translation="QTILEKEFSTALSIDTIADRVGMSPRNFKRKFKQVTGDAPSVYL
                     QRLRIEWARNRLAETGDSIESISYAVGYENVGFFRLLFKRHTGLTPFDYRRKNSRSSR
                     N"
BASE COUNT          996 a         1037 c         1064 g          977 t
ORIGIN      
        1 ggtaatttcc caggtatctg ggtgggattc tggactgggc ggacaaaacc cggattcttt
       61 cgatggcctg cctggcaggc cgcgccgggc aggggacaat gcagtttttg gcctcgtcgt
      121 aatactcata gggaggctcg ggggccttca caatggcctc caggcacctg cagagcgcaa
      181 aatcgcctgt cttcctgccc cctccgaggg ggtcaaaaac gatccccagg ccgttgcaga
      241 cgcggcagga tcggtctccc agcttctcgg cgtatgcctc gaggcgcgat gcgatcgtag
      301 gttctgtcaa atcatgctcc attttttcat aagatcattg atttatttgg cgccatcggt
      361 tgattcaggg cataagaggc ccgataggat acctgatcgg gaaaccatat attcctagat
      421 tcatgaaggt gcgaccatga caaagagaat tttatcgatt ctttgcctcc tcgtttttcc
      481 ggcagccgtc tttgcacagg cgccagctca gccagcacaa cctgctcagc cagcgaccac
      541 tccagcgacg acacagccaa ccgatgtggc gaatccagct aacccaactc cgtatgagcc
      601 aagcccaggt gaacttgtca ccgaggaagt caacgaagct gaacttagcc cggaacaaaa
      661 agaagtaaga gatagaaacc tgaaggcaga agaactctgg aagaatgctg actaccgcga
      721 ctacaaccgc gcgttcgcag aactgcacca gctttccaaa gcttttgcaa acaacaaata
      781 ccgtctatcg ctgtctgctt accagtctgg cgtaaatacc atcatcaaga tgcgtgacga
      841 agtggaactc tttcgcaaag agtccgcaga gacaaaacgt ctgaatgaaa aatggtactg
      901 gcagattgta gaccgcaaag cgaaagaaga gcgcttgctt tcaaatatga agcgcactgc
      961 aaagctcgaa gcggttacat actttacacg tgcaatcaat cacctggacg aaatcaagaa
     1021 ccctgacctt cgtgagaagc cagcgttcaa gaaacttctc gctgctgtat atcgcaactg
     1081 gatcatgtat cagtttgatc tgggcaacct gccacagacc atcccgatcc tcgaactcta
     1141 cattgagatc gaccagaacg agaaggaata cccatctcac aagtaccttg ctcaggctta
     1201 ctccttccag gagaacatga tcaagaagta cggagtgggc acagaagacc agatgttccg
     1261 cttccgttac aagaagaacg tgcaccttct gcgtgcgacc gagctgaaat acggcaagga
     1321 atctgcagaa tacaaacacg tagtgaatct tgtgaacaga gacgagatca tctcagttca
     1381 gccctgatca aggtccgcac ctcaaagaat gtaaagcccg gcagagatgc cgggtttttt
     1441 gtttttcaag gccactctgc aaaagttgac tgatccgggt ctgcgtcgtt tctactgaat
     1501 aattcttgcc atccaatcat actgccatta gaaatcgtcg gagtcacggt cgaatgcgaa
     1561 aaggacaaaa gctcagaacg gatgggagca aaggatcatt ggcggttcgg ggcaaactgc
     1621 agcttttttt gttcctgctt ctcttatggg cgggatgtgt accacagttt ccgggggatc
     1681 gcaagacctt tcagaccttg atgctttgcc tcgcttccag gtcagggatc tgcgtgcctg
     1741 aatatccttt caaccctgcc aatgtatctg ccatgcagtt ctggcttgcg gcagatgacg
     1801 ttgtgccctc cactggaaca gtcgtgtcgc gctggccgga tcgcagcgcg aacgcaaatc
     1861 atgttgtgca gtctaccgcc gcaatgcagc ccacttacac gacgggtgcg gcgccctact
     1921 ttcatcctgt agtgtacttt gatggcgggg atcaactgac cggtttgaat acagcactca
     1981 ctggcgccga tttcactttc tttgtagtat acaacagacc agttgcgggc gggcatccgt
     2041 ttctagaggt ggggcaggtc actctcacca atgcgaaatc ccttggcgtg aatggatccc
     2101 tgggcgtaga tgtttgttct cgcaatgttg cctgcgcgcc gggtccagcg ggcgttacat
     2161 atacaaacaa cgcatggcac gcagtcacgg tcacatacag cggatcgggc aatcagaagc
     2221 aaacccagtg gcgggatgga gcgagcgtgg ggtccggtac agcgcaggcg cttgcatttg
     2281 atactgtggg ctttgcgtcg gtaggcaacg caccaaacgg cggggcgggt ttcgtgggct
     2341 atatagctga aatcatttac tacaatcgta ttctctcttc ttcagagatt gactacgtga
     2401 attgctatat gactttgaaa tttgccattg gctccgctct gttttgcaag taagctcaag
     2461 gccaacgctt agaatttgtt gaagaacgga gacatgagta tgttttcgca cagagttccg
     2521 cgagcgtttg tctgcaaata ctgaaaagga acacttgcac tgcgcgggat ggtgtaaatt
     2581 cttcctgtag gaaccagaat ccctgacgtt tgggctgttg caccacccgc tgatatgcca
     2641 gtggccacga cagggcccat gctcaatgta tcaatcgtgg cgctgccgcg cgggctgccg
     2701 taagtgattc cgtttggtcc aacaacattt ccttcgaact ggttgagcgg gtaggtggtt
     2761 ccgaaatagg acaccgtcct tgcgaaagga tcaaaaacca tagactgatg atttcccaca
     2821 ggcagggaat agaaacgacc gtcaggcgcc attcctcctc caaagaaacc ctgggtgcct
     2881 gcaaaacttg cgaaagtcga aaccgaatca ttggttggat caatctctag aaagcccacc
     2941 gcggcgatgt ttcccatcgg aaaggcgtat attttccctg ttaaggccag cactccgcca
     3001 atccaacctc cggtcgttgc acctgatatg gacgtgaact ggtttgtaga tggatcaaat
     3061 actgctacag gtagtccggc cgtcataggt atcgcataga tttttccatt gggcgcaagc
     3121 actgcaccgc cgtaacccgc cgtaacactt ccaaatgtac tgagagcatt tgtggtagga
     3181 tcgatcgaga ggatgctggt ggtgccactg atcgggaaga agtacatttt gccattggca
     3241 gcaagagctc ccggactcca ttttgggccg gcgcctccaa tgccggctat cgtggatgtg
     3301 atgtttgtct ttgggtcaat gatgagagct tgattgttga ttgcattttg atcattggga
     3361 ggggcgtaga tttttccatt aggcgccagc aatccacctg agccataaaa gtcattggca
     3421 cctcccagtg tgccgtactg tacaggcgtt ggatttccgg tagatccttt ggatgcttga
     3481 tagttatatt ccagcatcat gcttccgcga aagagtggat ccgagatctt gtaatagggg
     3541 acgcggcaga tgtcgtacat gatcaggttg gccattagct tgcaggatac gtccgtgtcc
     3601 ccacattgct ggtgctgcga gcaccccgtg cccgaaagcg tgaataagac gaagccgatc
     3661 gcgcataacg ttcgccggac ggggcatcgc atagcgcatt tgaagtgaac ctgcaggatc
     3721 tgaaaagaag aatcctggtt ttgcgacaaa ggagtcgcgt ttaattgcgc gaagacctgg
     3781 agttttttct cctgtagtca aatggagtca ggcccgtgtg tctcttaaag aggagtcgaa
     3841 agaatcctac gttctcatat cccacggcgt agctgatgct ttcaatggag tctccggttt
     3901 ccgccagtct gtttctggcc cattcaatgc gaagccgttg caaatagact gagggcgcgt
     3961 ctcccgtcac ctgcttgaat tttctcttga aattcctggg actcatgccc actcgatctg
     4021 cgatcgtatc aatagagaga gcagttgaga attccttttc gaggatcgtc tgcg
//
LOCUS       DAOQJL010000358         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13501_length_4057_cov_4.633933, whole genome shotgun sequence.
ACCESSION   DAOQJL010000358 DAOQJL010000000
VERSION     DAOQJL010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13501_length_4057_cov_4.633933"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PLG78_14075"
     CDS             <1..2127
                     /locus_tag="PLG78_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24297.1"
                     /translation="VSRDSMKLRLPTEKTYRFSILQALLPIVIISIVGISLNFIIGST
                     RAVVELSDHLVEEVSQRMIERTLAHLEGGAMHNRVTANLVSDLDLMNNHETVWRYLWA
                     PMIETPQVQSLFVADRRGNYVQVRREPFLATRMIDAARGIETTVTRDESYREKETKTN
                     PTTWDPRTRPWYKNTTREKKMAWTDVFVFTTAKTPGLSASYPVLDAQGEIVGVIAANI
                     PLHNLSDFVAEQKVMQHGIVLIINDKGEVVAYPDKKVNLVVDSDAKEPDPKEHKEKEM
                     RLLRIDELPNQAVVDAYRRSESEKSTRVTSATNGEKYITTFKPFPAPFEKLKIVIVIP
                     EADILASIHRMELIAILVSVVILILGIIVVYQVSIRISDPIIRLAHEMDHIGDFSLDD
                     VKGVQSKIHEIRIMNSSLLTAVEGLKAFRRYLPASFVRQLITSGKKAELGGIKTEITL
                     FFSDVEGFTRISESMPAEDLMLHLSAYFNELSRIILEEGGTIDKYIGDAIMVFWGQPV
                     EIADAPIRACRAALRCVERLTELNEKWSAEGKPTMRTRFAIHTGESIVGNVGTEERMN
                     YSAVGDSVNLTSRLESANKIYGTTIIISQSTFERVQHLFRCRLLDVVAVRGKTRGVRI
                     YELLGESTVSPTEAQNRFVAGYEQAFSVYLSRNWQEAVQAFSKLNVEFPADKSVQVML
                     RRCDAFAKDAPGLPPNWDGTMAMLEK"
     gene            2687..3655
                     /locus_tag="PLG78_14080"
     CDS             2687..3655
                     /locus_tag="PLG78_14080"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNE24298.1"
                     /translation="MDAYQVKGTNWWGAIDAEGRLLWKREDIHWLGAFVEGYAICVNR
                     EFRTGLITATGEVVIEPKYSLLRDYACGLVAYRETEKGKFGYLDNTGKVVIPPKYLSA
                     NPFSVEGLAIVQKGKQVRVCIDRNGTELWEIEAEACASFSEGLAPYLVKKGGWGYVDI
                     SGQSVITPAHRLAGPFSEGLAGVGSGRGKYTFINKHGERLPEALGLVSGWDFSEGLAS
                     VETKSSQGLVGFIDRAGNVEIPPQFVNAGSFKGGLAPVKLPVALGESREWAGFIDRSG
                     RLVFGHDHNLAFTEEFVGHLARVRTVDGRSGYLNRSGRLIWIMEDS"
     gene            complement(3827..>4057)
                     /locus_tag="PLG78_14085"
     CDS             complement(3827..>4057)
                     /locus_tag="PLG78_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24299.1"
                     /translation="DGCWVGGHSSEMAFSFETFSVYARSVEGGGRCPVSCDLLKGCPV
                     HLTETTTVKYFANGKETQASHFLVNLFKIAEK"
BASE COUNT         1012 a          954 c         1105 g          986 t
ORIGIN      
        1 gttagcaggg actctatgaa attgcgccta ccgaccgaaa agacatacag attcagtatt
       61 ctccaggcac tgctccctat tgtgatcata tctatcgtcg ggatttcgct caacttcatc
      121 attggctcca ctcgcgctgt agtagaactc tccgatcatc tggtagaaga agtaagccag
      181 agaatgattg agagaactct agcccatctc gaaggcggcg cgatgcacaa tcgcgtgacc
      241 gcaaatcttg tttctgatct agacctgatg aacaaccacg agacagtgtg gcgatatctc
      301 tgggcgccca tgatcgaaac acctcaggtt cagagtctgt tcgtcgctga tcgaagggga
      361 aactacgtgc aggtgcgtcg cgaacctttt ctggccactc gcatgattga tgctgcgcgc
      421 ggtatcgaaa ctacagtcac tcgcgatgaa tcatatcgcg aaaaagaaac gaagacaaat
      481 ccaactacct gggatcctcg caccaggccc tggtataaga atactaccag ggaaaagaaa
      541 atggcctgga cggatgtgtt cgtctttacg actgcgaaga cacccggtct ttccgcttct
      601 tatccagttc tggatgcaca gggagaaatc gtaggagtca tcgcggccaa tatccctctg
      661 cacaacctgt ccgattttgt agcggaacag aaggtgatgc agcatggaat tgtgctgatc
      721 atcaacgaca aaggggaggt cgtcgcctac ccggacaaga aagtgaatct ggtcgtggac
      781 tcagatgcaa aagagccgga tccaaaagaa cacaaagaaa aagaaatgcg ccttcttcgc
      841 attgatgaac tgccaaatca ggcggttgta gatgcgtatc gcaggtcgga gagtgaaaag
      901 agcactcgcg taacatctgc gacaaatggt gaaaaataca ttactacttt taaaccattc
      961 ccggcgccct ttgaaaaatt gaagattgtg attgtgattc cggaggcgga catccttgca
     1021 tccattcaca gaatggagct gatcgcgatt ctcgtgtctg tagtgattct gattctgggc
     1081 atcatcgttg tgtatcaggt gtcgattcgc atttcagatc caatcattcg tcttgctcac
     1141 gagatggatc acattggcga tttcagtctg gatgatgtca aaggagttca gtccaaaatt
     1201 catgaaatca gaattatgaa tagctccctt ctgacagcgg tggaaggatt gaaagcgttt
     1261 cgcaggtact tgccagcgtc cttcgtgcgt cagctgatta cttccggcaa gaaggcagag
     1321 ctcggcggaa tcaagactga gatcactcta tttttctctg acgtggaagg gtttactaga
     1381 atttctgaaa gcatgcctgc ggaagatctc atgcttcatc tgtccgcgta ttttaacgaa
     1441 ctttcgcgca tcatcctgga ggagggcgga accattgata aatacattgg ggatgccatc
     1501 atggtgttct ggggacaacc ggttgaaatc gcggatgcac ccattcgagc ctgtcgagca
     1561 gctcttcgtt gcgtagagag actgacagag ctcaacgaga aatggagtgc agaaggcaag
     1621 ccaaccatga gaactcgctt tgcaattcat acaggggaaa gcatcgtagg caacgttggc
     1681 acagaagaga gaatgaacta cagcgcagtg ggtgatagcg tgaacctgac ttctagactg
     1741 gaaagcgcca acaagattta cggaaccacc atcatcatca gccagagtac attcgaacga
     1801 gttcagcatt tgtttcgctg ccgccttctc gatgtggtgg cggtgcgcgg aaagacacgc
     1861 ggagttcgca tttacgagtt gttaggcgaa tctaccgtat ctccaacgga agcgcaaaat
     1921 cgatttgttg caggctacga acaggcattt tccgtctatc tgagtcggaa ctggcaggaa
     1981 gcagtgcagg cattctctaa actgaatgtg gaatttccgg cggataagtc tgtccaggta
     2041 atgcttcgcc gctgcgatgc gtttgccaaa gatgcgcccg gtctgcctcc gaactgggat
     2101 ggcacgatgg ccatgcttga gaaatagctt tgaagcgctg tcaacgaata tgattgacgc
     2161 aagtcagtgg tagcggcata tttagaatat gcaatcgagg actgcagccg ctgcgcgaga
     2221 gttgcaaaat gctttcgtgc tgaagctcac cgctcgtgaa cgaattaagc tggccttgcg
     2281 ccttggacgc agggccgccg ccttgaaagg gaagaagaat ggacgcgggt gaacgcacaa
     2341 tcgatgccgc ccttgacgtt agccggattc ttgacgagtt acagattcgc catgcaatga
     2401 taggcgcggc tgtgtgcgtc acggccttgg cacggagtgg aatgatttcc aaagattttt
     2461 gtcaggctga gatctcacaa ttgccggaaa ggcaaacgtt agtcggtcgt ccgcaatgag
     2521 tcaggcagag cagcatgtag aggattgcgt atgcctgttg ggtaatccac ctcctctttt
     2581 gggacgcggc gctttcttgt gaccggaaaa ccttttcctc gcagttctca aacacggccg
     2641 ggttttcggt ttgcacccta attcctcaag cggattttga gcttgtatgg acgcttatca
     2701 agtgaaagga acgaattggt ggggcgccat cgatgcggag gggcgactcc tctggaaacg
     2761 cgaggacatt cactggctag gagcatttgt ggaaggttat gcgatctgcg tgaatcgcga
     2821 gttccggacc gggcttatca ctgcgacagg cgaggtggtc attgaaccaa aatactcgct
     2881 tctgcgcgat tacgcatgtg gtctggttgc atacagggaa actgagaaag ggaagttcgg
     2941 ttacctcgac aacacaggaa aggtggtcat tccgcccaaa tatctaagtg caaacccttt
     3001 cagcgttgag gggctagcca tcgtacagaa gggaaaacag gtcagagttt gcatagacag
     3061 aaatggtacc gaactttggg aaatagaggc tgaggcttgt gcctcattct ctgaaggcct
     3121 ggcaccgtac ctggtgaaga agggcggttg gggttacgtg gatatcagcg gccagtcagt
     3181 gatcactccg gcgcatagat tggccggccc attttctgag ggtcttgcag gggtcgggtc
     3241 aggccgtggt aagtatacat ttattaacaa acatggagaa aggcttcccg aggcactcgg
     3301 tttggtatcg ggttgggatt tttccgaagg cctggcttcg gtcgaaacaa aatcctcgca
     3361 aggcctggtt ggtttcatcg atcgcgccgg taacgtcgaa attccgccgc aattcgtcaa
     3421 tgccggctcg ttcaagggcg gccttgcccc agtgaagcta cctgtggcat tgggggagag
     3481 tcgcgaatgg gctggattca ttgatcgatc tggccgtttg gtttttgggc atgaccacaa
     3541 ccttgcgttt actgaggagt ttgtcgggca tttggcgcgc gtgcggaccg ttgacggccg
     3601 gagcggttac ttgaaccgct ccggcagact catttggatt atggaggatt cctgaactcc
     3661 ttcgtcacat agcgtccgtc aatccaatac tcgtacctaa tggtgcttgg tcttgtcgga
     3721 ttggatccat tatactcagg agttactttc acacgggctt tccctgaact gctttctcta
     3781 atctgtcggg caagattctc cctggtttca gggattgcgg agacgcttat ttttccgcaa
     3841 tcttgaatag attgaccagg aaatgagatg cttgtgtttc tttaccattt gcaaaatact
     3901 ttaccgtggt ggtctcagtc agatgcaccg ggcatccttt taataaatcg cacgaaacag
     3961 ggcagcgtcc gcctccttcc actgagcgag cgtagacaga gaaagtctca aaagaaaacg
     4021 ccatttcaga agagtgacca cccacccaac atccgtc
//
LOCUS       DAOQJL010000359         4008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13729_length_4008_cov_4.026815, whole genome shotgun sequence.
ACCESSION   DAOQJL010000359 DAOQJL010000000
VERSION     DAOQJL010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13729_length_4008_cov_4.026815"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLG78_14090"
     CDS             complement(<1..921)
                     /locus_tag="PLG78_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE24300.1"
                     /translation="MRFLILFASSTMLSAQKLLSVAALGLFAFCGKSKEVPPINPTKV
                     FTQVEAAITTTYFSGDPAPPLEKLYGNAVAALGAKETPDMKGTIKAIESQGPDKVRER
                     TYRALNAFLSELPGGNDFTRAEGLLMSEDKSRQAGIGILLMQEGPGKFIVLDTLESSP
                     AQAAGVKPGSVVKSIDGLEVANMDLEEVAGRIRGAESTDVRLGLLGSEPTIKRSKLNF
                     INYSNATWLLTTGGQVEIVALRTAVPGTATNLEQFMRQMGKRSAVILDLRKLQSGDFS
                     ECFKIADLLVGKRKMGELVQKNGAIPMESTA"
     gene            complement(956..1189)
                     /locus_tag="PLG78_14095"
     CDS             complement(956..1189)
                     /locus_tag="PLG78_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24301.1"
                     /translation="MFRIALVTALALGMACGNTFETHACHRDCSNRYNSCLTTMVLLN
                     VPTNQSPAFNTAGVLSCNTDHDNCNATCSQLGD"
     gene            complement(1182..2330)
                     /gene="aroC"
                     /locus_tag="PLG78_14100"
     CDS             complement(1182..2330)
                     /gene="aroC"
                     /locus_tag="PLG78_14100"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE24302.1"
                     /translation="MSSVAGTLFTISTFGESHGPAVGVVLDGCPAGFEIDLEELQAEL
                     DRRKPGQSHLTTSRQEPDRVEILSGLFEGRTLGTPIAMMVRNVDMRSQDYEGFSQAYR
                     PSHADFTYHAKYGFRTPHGGGRASVRETIGRVAAGAIARQILRKELGIETIAWVESIG
                     NIDSQAIKNPPQSREQVDSNLVRCPNEIAAKKMVALVEEARAKKDSVGGTLGVIVRNV
                     PPGLGDPVFDRLEADFAKASLSIPACKGFESGSGFEGTRLFGSQHNDSFIPKENPREG
                     ATETMPAPNVETKTNFSGGIQGGISNGMPILCRLAFKPTATISQPQDTVNDRGEATEL
                     AAKGRHDPCVLPRAVPIVESVFNLVLMDAYLRQRAQTPEWWLRYRKNV"
     gene            complement(2366..3337)
                     /locus_tag="PLG78_14105"
     CDS             complement(2366..3337)
                     /locus_tag="PLG78_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNE24303.1"
                     /translation="MLPHNKLISSLGGAKQVYIQTHNFPDHDAVATAYGLQYFLKRAG
                     IQANVIYEGEVQRDSLKRMIQELNIDVRPASRYALTAEDKIIVVDGCKGNKNVTDLIG
                     DEVAVIDHHQVTAPDDVKFADIRPEYGACSSIIYEYLNTQEGQIPQNVATALMVGINM
                     DTSLLTRSVSKGDLLAYTELYGLADMPLVNSILINYIQTKDLAFYKTALDRVRIKETL
                     AFCYFPDGCNQNLMGILGDFFMSLQEIEFVALCAKNDGKINFSLRSENRSWNASKVIQ
                     EVLEGVGFGGGHNDRAGGIIQDAALFHEEEIYKKVVRALKLPDIAIF"
     gene            complement(3373..>4008)
                     /locus_tag="PLG78_14110"
     CDS             complement(3373..>4008)
                     /locus_tag="PLG78_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24304.1"
                     /translation="LWRIDSIARQTARFILHILKQRPPQAPQRTNKVDQSPQILTHSM
                     GGLIAMHAVEYLQDHDLEVPFKRMHFVAPGALRFSESDRDAHRKRFYPTSTDEIRELL
                     GELFHETRTDLPDFLLRGLLEQWSHKGYEYLAENTIEEEDSVFYPSGKRIKLSPEIIL
                     YWGREDRIVNLQYGKLVRMTLRCRLIEMQGGHCVQTENPDELMRAFLANCK"
BASE COUNT          872 a         1029 c         1013 g         1094 t
ORIGIN      
        1 agctgtactt tccatgggaa tggctccatt cttttgcacg agctctccca ttttcctttt
       61 tccgacaagc aggtcagcta tcttgaagca ttcgctaaaa tcgccactct gtagcttgcg
      121 aagatccaaa ataacagcag accgttttcc catctggcgc atgaactgtt ccaggttcgt
      181 cgcggttccg gggaccgctg tgcgaagagc cactatttct acctgtcctc ctgttgtcag
      241 caaccaggta gcatttgaat agttgataaa atttagtttt gaacgtttga tggtgggttc
      301 gcttccgaga agcccaagtc gtacatctgt gctttccgcg ccgcgaattc gaccggccac
      361 ttcctcaagg tccatgttcg ccacttctaa accgtcaatg ctcttcacta cggaacccgg
      421 tttgactccg gctgcctggg cgggagaact ttccagtgta tccagaacga tgaattttcc
      481 aggcccttcc tgcatgagaa ggatgccaat tcccgcctgc cgtgatttat cttcgctcat
      541 caagagccct tctgcgcgcg tgaaatcatt tccgccagga agttcggaaa gaaatgcatt
      601 cagtgcgcga tacgttcttt cgcgtacttt gtccgggccc tgcgattcga tagccttgat
      661 cgtgcctttc atgtctggcg tttctttcgc accgagtgcg gctacggcat ttccgtaaag
      721 cttctcaaga ggcggagccg ggtcgccgga gaaataggta gtagtaatgg cggcttccac
      781 ctgggtgaat actttggtgg ggttgatggg tgggacttcc tttgattttc cgcagaaagc
      841 gaaaaggccc agagcggcca cagacaggag tttttgtgct gagagcatgg tactggatgc
      901 aaaaaggatt agaaatcgca agtgatttcc gggcggcggg aaagaacctg aaacgtcaat
      961 cgccaagctg gctgcacgtc gcgttgcagt tgtcgtgatc tgtgttgcag ctcagtacgc
     1021 ctgctgtatt gaacgcaggg ctctgatttg taggaacgtt gagaagaacc atggtggtca
     1081 ggcagctgtt gtagcgattg gaacagtctc tatggcaagc gtgggtttca aatgtatttc
     1141 cacacgccat acccagagcc agcgcagtaa ccagagcaat cctaaacatt ttttctatat
     1201 cgcagccacc attcgggagt ctgcgccctc tgtctgagat atgcgtccat gagaaccagg
     1261 ttgaaaacag attccacgat ggggacagcc cttggcaata cgcatggatc gtgcctgccc
     1321 ttcgccgcaa gctctgttgc ttcgccgcgg tcgttcactg tgtcctgagg ctggctgatg
     1381 gtggccgtag gtttgaatgc gaggcggcat agaattggca ttccgtttga gatgcctccc
     1441 tggattccac cggaaaagtt cgtttttgtt tccacattgg gagcaggcat tgtttccgtt
     1501 gccccttcgc gcggattctc tttgggaata aacgagtcgt tgtgctggct tccaaacagc
     1561 ctcgtgcctt cgaaaccgct cccggattcg aaacctttgc atgcagggat agagagagat
     1621 gcttttgcga agtccgcctc caggcgatca aacacaggat cgcccagacc gggtggcacg
     1681 tttctcacaa tgacgcctaa cgttcctcca actgagtctt tctttgccct tgcttcttct
     1741 acgagtgcaa ccatcttctt cgcggcgatt tcatttggac aacgcactag atttgagtct
     1801 acctgttcgc gcgattgcgg aggattcttg attgcctggg agtcaatgtt cccaatggat
     1861 tcaacccacg cgattgtttc tattcccagt tccttccgta aaatctgtcg tgcgattgct
     1921 cccgccgcga ctctaccgat tgtttcgcgc acggaagccc ttcctccccc gtgcggagtg
     1981 cgaaaaccat attttgcgtg atacgtgaag tccgcgtgag aaggacgata tgcttgcgag
     2041 aatccttcat aatcctggga gcgcatatct acattgcgaa ccatcatggc aatgggcgtt
     2101 ccaagagtgc ggccttcgaa caggccgctc aatatttcta ctcgatccgg ttcctgtctc
     2161 gatgtggtga ggtggctctg gcctggtttt cgtctgtcca gttctgcctg caattcttca
     2221 agatcgattt cgaatccggc cggacaaccg tcgagtacga cacccacagc cggaccgtgc
     2281 gattcgccaa aagttgaaat ggtgaagagt gtgccggcaa cagaagacac gatcctgtat
     2341 cagccgcggg gcgggcctgt caaattcaaa agatcgcaat atcaggtagc ttcagtgcgc
     2401 gcactacttt cttgtatatc tcttcctcgt gaaaaagggc cgcatcttga atgattcctc
     2461 cggctcgatc gttgtggccg ccgccaaacc caactccttc gaggacttcc tggatgactt
     2521 tggacgcgtt ccatgatcgg ttctcactgc gaagtgagaa attgattttg ccgtcattct
     2581 ttgcgcagag agcgacgaat tcgatttctt gcagagacat gaagaagtct cccaggatgc
     2641 ccatgaggtt ctgattgcag ccatcaggaa aatagcagaa tgcaagcgtt tccttgatgc
     2701 gaactcgatc gagtgccgtt ttgtagaacg ccagatcttt ggtttgaatg tagttgatca
     2761 ggatcgaatt gacgaggggc atgtccgcca gtccgtacag ttccgtgtaa gccagcagat
     2821 cgcctttgct tacgcttctg gtcaacaagg atgtatccat gttgattccc accatcaatg
     2881 cggtggccac gttttgtgga atttgtccct cttgagtatt cagatattcg taaatgatag
     2941 acgaacacgc accatactct gggcgaatgt ctgcaaactt cacgtcatcc ggcgctgtga
     3001 cctgatgatg atcgatcaca gccacttcat ctccaatcaa atctgttacg ttcttatttc
     3061 ctttgcaacc atctacgacg atgatcttat cctccgcagt gagtgcatag cgactggccg
     3121 gtcgcacgtc gatgttcaat tcctggatca ttctcttaag agaatcgcgc tgtacttctc
     3181 cttcgtaaat gacattcgcc tggattccgg cgcgtttcaa aaagtactgc agaccatagg
     3241 cagttgccac cgcgtcgtga tcggggaagt tgtgcgtctg gatgtaaacc tgtttggctc
     3301 caccgaggga tgagatcagt ttattgtgcg gaagcattac caatgaaaaa acgcaacgag
     3361 tttcaatgca acctatttgc agttcgcaag aaacgcgcgc atcagttcgt ccggattttc
     3421 cgtttgcacg caatggccac cctgcatttc gatcaagcgg catctcagag tcatccgaac
     3481 cagctttcca tattgaaggt tgacgattcg atcttcgcgg ccccagtaca gaatgatttc
     3541 tgggctgagc ttgattcttt ttccggaggg atagaataca gagtcttctt cttcaattgt
     3601 attttctgcc agatattcgt accctttgtg ggaccattgt tcgagtagtc cgcgcaacaa
     3661 gaaatcaggt agatccgttc ttgtttcgtg gaaaagctct cccaggagtt ctcgaatttc
     3721 atccgtgctc gtaggataaa atctctttct gtgtgcgtct ctgtccgatt ctgaaaatcg
     3781 cagtgcgcct ggggcaacaa aatgcattct tttgaacgga acttcgaggt cgtgatcctg
     3841 cagatactcg actgcatgca tagcgatcag tcctcccatg ctatgcgtca gtatttgcgg
     3901 agactgatct accttgtttg ttctctgcgg cgcttgcggg ggtctttgtt tgagtatatg
     3961 aaggatgaag cgcgctgtct gccttgcgat gctgtcaatg cgccacag
//
LOCUS       DAOQJL010000360         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13819_length_3989_cov_4.781901, whole genome shotgun sequence.
ACCESSION   DAOQJL010000360 DAOQJL010000000
VERSION     DAOQJL010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13819_length_3989_cov_4.781901"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="fliI"
                     /locus_tag="PLG78_14115"
     CDS             complement(<1..274)
                     /gene="fliI"
                     /locus_tag="PLG78_14115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HNE24305.1"
                     /translation="MEVRGLSLISDGPPDASIGDICRVAVKSGDYIRAEVVGFQKDRL
                     VLMPLGGTAGISPSADVLASGRRMQIAVSEKMLGRVFDALGHPLDMK"
     gene            471..1346
                     /locus_tag="PLG78_14120"
     CDS             471..1346
                     /locus_tag="PLG78_14120"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE24306.1"
                     /translation="MDEDHILTLLTEHALIREDQAARVKEIHASTGRPTGSILLSERF
                     LDEKQLYAFICQDLGMNPASVYQDKFYEISGLTIPKFEVSGDFYGSFMLDEGRIALTL
                     SDVSGKGIEAGLLAILLANILRTGVRMKNIIPSMILRKVNHASTRFFGEEQFATFIVL
                     ILDMHSGTVEFCCAGSPPILVYRRRENYVEQLEVKGIPVGIYDDFLFNGSRTSIEKGD
                     VILMYTDGAYENQNFRNEYFGLRRVKDSLRKNSPRTARKIIHAIKREIKLFSMFRGLN
                     DDTTFVSIKRSDRGS"
     gene            1343..3037
                     /locus_tag="PLG78_14125"
     CDS             1343..3037
                     /locus_tag="PLG78_14125"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNE24307.1"
                     /translation="MKELAGVCAAKGIARGFIHILPPLSRAVPNHQVSEESIPQELDR
                     LEVARSSSVKEIEEILIESDLPAEHREIFDAQILLLADPMLFDTAKNRILNGKQNAAG
                     ALADTLDDFKRQFEKSPDPFFRERIADMEDVSTRVLSHLLGTAQGDPRLPFLKKLPAD
                     AILAADEIPPSLMLHIRKVAGLAVEQGGVTGHMAILARDRGLPALVSVEGLLENVKEG
                     QPALLNATENWIRLEPEQTDYAAMQGTQSKVRRVQSPVSLRGGGSARIWVNLDDPEDA
                     SEESVQAAHGIGLFRTEFLFYKYPDLFDSPDDHTEVYRTVFRSLRGKPVTCRLLDVGD
                     DKALPPVFNRYLAADKQDAILRGVRFLLSHPGLLHLQLKALLEAALDEGMQTEHLRIL
                     VPLVSTRGEILEFREYYDAIRKEVSKERGIACPEFPVGIMMETPAACVMSDVLSRYAT
                     FFSLGTNDLAGLALAFHRSGPSEDPFFQPSLFRLIQTGLKGASIPVSICGEMAAIPEI
                     VPILVEMGIHDFSVSISSLADISDALAAYDPQKNAGKLARILNAQNREEVRSILLE"
     gene            complement(3042..>3989)
                     /locus_tag="PLG78_14130"
     CDS             complement(3042..>3989)
                     /locus_tag="PLG78_14130"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE24308.1"
                     /translation="HATVHEDGLRIDRLMCLRFPVLGREDWQDRIRTGQVLLEGRTVR
                     PSAKVKSGSQIVFRYERRPEPEVNRAIEVLWEDHESMVIHKPANLPVHPSGIYFENTL
                     TTILRSMFPDHTPLLVHRLDRETSGLLLLAKTKESASRLQKQFLSSTITKEYLVLVEG
                     QFPDSLDARGFLGPAGSEVKKRMKFARVEFPGSVSSHTGFSLVGQGQIDSGSCISLIR
                     AILHTGRMHQIRATLCSLGFPVVGDRLYGVDETMYLRFVRGEESSTDRELLRMDRTAL
                     HSARLIFESGGKTIQVDSNPPQDFVDVMRQFGLSVSKEG"
BASE COUNT         1008 a         1032 c         1009 g          940 t
ORIGIN      
        1 ccttcatgtc gagaggatga cccagggcgt caaagactcg accgagcatt ttttcgctca
       61 ctgcgatctg catccgcctg ccggacgcca gcacgtctgc ggaaggtgaa attcctgccg
      121 ttcctccgag aggcatgagc accaggcgat ccttctggaa gccaactact tctgcgcgaa
      181 tgtagtcgcc agatttcacg gcgacgcgac atatatcacc tatgcttgcg tctggcggac
      241 catctgaaat gagcgaaagt ccgcgaactt ccactacccg gcccatcggg cgcagtgggt
      301 cgagccggtc caggatatgg cggtacttgt ctagtctcgt taaatccttt tcatctaaaa
      361 tggccatagt gacgtagccc ggtgtattct tggatgccgg atgccgattt tcagtacaat
      421 cgttttttag tatccgatgg aaaatttatg tctcaaatgg cgatagaggg atggacgaag
      481 atcatatttt aaccctcctg accgaacatg cactgatccg cgaggatcag gcggctcgcg
      541 tgaaggaaat ccatgcctcg acagggcggc caacaggcag cattctgctg tccgagcgtt
      601 tcctggacga aaagcagctc tatgctttca tctgccagga tctggggatg aacccggcca
      661 gtgtttatca ggacaagttc tacgaaatca gcggtttgac catcccgaag tttgaagtga
      721 gcggagactt ctacggatcc ttcatgctgg acgagggtcg cattgcgcta acgttaagcg
      781 acgtatctgg aaaaggcatc gaggcaggct tacttgccat tctgctggcc aacattctta
      841 gaacaggcgt gagaatgaag aatatcattc cttcgatgat tctgcgaaag gtaaaccatg
      901 caagcactcg cttcttcgga gaagaacagt ttgctacatt cattgtgctc atactggaca
      961 tgcacagcgg tacagttgaa ttctgctgcg caggcagccc tccgattctt gtataccgga
     1021 gacgcgaaaa ctatgtggag caactggaag tgaagggcat tcctgttggc atttacgacg
     1081 atttcctttt caacggaagt agaacatcca ttgaaaaagg ggacgtcatt cttatgtata
     1141 cggacggcgc ctacgagaac cagaatttca gaaatgaata ctttggcctc agaagagtca
     1201 aagacagcct cagaaagaat tcgcctcgca ctgcgcgcaa gatcatccac gcgatcaaac
     1261 gcgaaatcaa gctcttctcg atgtttcgcg gcctgaacga tgatactacg tttgtctcca
     1321 tcaaaagatc ggatcgaggc agttgaaaga actcgcaggc gtttgtgcgg ctaaaggcat
     1381 cgcccgcggt ttcattcaca tccttcctcc gctctctcgc gccgttccca atcatcaggt
     1441 aagtgaagaa tcgattccac aggaattgga tcgcctggaa gtggcacgat cgagcagcgt
     1501 aaaagaaatc gaagagatcc tgatcgaatc agatctaccc gcggagcatc gcgaaatatt
     1561 cgacgcgcag atccttctgc ttgccgatcc gatgcttttt gacacggcga agaatcgaat
     1621 tctgaatgga aaacaaaacg cagccggtgc acttgcagac actctagacg acttcaaaag
     1681 acaatttgaa aaatctccgg atccattctt tcgcgaacgc attgcggaca tggaagatgt
     1741 ttccactcgc gtgctttcgc accttcttgg aacggctcag ggagatccac gccttccttt
     1801 cttaaagaag ctaccggcgg acgcaatcct cgctgcggat gaaatcccac cttcattgat
     1861 gttgcatatt cgcaaagttg cgggcctggc cgtggaacag ggcggagtaa caggtcacat
     1921 ggccattctt gcgcgcgatc gcggactgcc ggcccttgtg tcagtagagg gtttgctcga
     1981 aaacgtaaaa gaaggacagc ccgcacttct caacgcaact gaaaactgga ttcgcctcga
     2041 accggaacaa acagattacg cagccatgca aggtacgcag agtaaggtaa ggagggttca
     2101 atctcctgtt tcgctgcgcg gaggaggctc ggccaggata tgggtgaacc tcgacgatcc
     2161 agaagacgcg tcggaagaaa gtgtacaggc cgcgcacgga atcggtttgt ttcgcacgga
     2221 atttctcttt tacaaatatc cggatttgtt cgattccccg gacgatcaca ctgaagtgta
     2281 tcgaactgtc ttccgttctc ttcgcggaaa gccagtgacg tgcagacttc tggatgtagg
     2341 cgacgacaag gctcttcctc ctgttttcaa tcgttatctc gcagcggata aacaggacgc
     2401 gattttgcgt ggcgttcgtt ttctactctc tcacccggga cttctgcatt tgcagctcaa
     2461 agctttgctc gaagcggccc tcgacgaagg aatgcaaaca gagcatctgc gcattctggt
     2521 tcctcttgtt tccactcgcg gtgagattct agaattcaga gaatactacg atgcaattcg
     2581 caaagaagtt tcgaaagaac gcggcatcgc ctgtcctgaa tttccggtgg gtatcatgat
     2641 ggagacgccg gcggcctgcg tgatgtctga cgtattgtcc cgttatgcga ccttcttctc
     2701 actgggcaca aatgatctgg ccggactcgc acttgcgttt catcgttcag gtccttccga
     2761 agatccgttc ttccaaccat cgctatttcg tttgatccag actggcctca aaggtgcgag
     2821 catcccggtc tcgatttgtg gagaaatggc cgcgattccg gaaatcgtcc cgattctagt
     2881 ggaaatggga attcatgact tcagtgtttc catttccagt ctcgcagaca tatcagacgc
     2941 tctggcagcc tacgatcccc aaaagaacgc gggcaaactg gcaagaatac tcaacgctca
     3001 gaatcgagaa gaagtacgat ccatactgct cgagtaagac tttaaccttc ttttgacacg
     3061 gaaagaccga attgccgcat aacgtccaca aaatcctgcg gaggatttga atcgacctga
     3121 attgtttttc caccggattc gaagatcagc ctcgcgctgt gtagtgctgt gcgatccatg
     3181 cgaagaagct cgcgatcagt ggacgattct tctccccgaa caaaccgcaa gtacatagtt
     3241 tcatccacgc catagaggcg atccccgacc acaggaaaac cgagagagca aagagtcgca
     3301 cgaatctgat gcattctacc cgtgtgaaga atggcgcgaa taagactgat acaggaacca
     3361 gagtcgatct ggccctggcc gaccagagag aaaccagtat gagacgaaac agagcctgga
     3421 aactcaactc ttgcgaattt cattcgcttc ttgacttcgc tgcctgcggg accaagaaat
     3481 cctctggcat ccagggagtc tgggaattgc ccttccacta aaaccaggta ttctttagta
     3541 atcgtcgaag acaaaaactg tttctgtagt cgcgaagcag attctttggt ttttgcaagc
     3601 aacaagagcc cgcttgtttc gcggtccagg cgatgaacga gcaatggagt gtgatccgga
     3661 aacatgctcc gcaaaatcgt agtgagagta ttttcaaaat aaatgcccga aggatgaacg
     3721 gggagattgg ccggcttgtg aatgaccatg gactcgtggt cttcccataa aacttcaatc
     3781 gcacgattga cttcaggttc tggtcttcgc tcgtatctaa atacgatctg gctgccggat
     3841 ttcacctttg cagacggccg gactgttctt ccctcgagca gaacctgccc ggtccgaatt
     3901 cgatcctgcc agtcctcgcg gccaaggaca ggaaagcgaa gacacatcaa gcgatcaata
     3961 cgcagcccat cctcgtggac agtggcgtg
//
LOCUS       DAOQJL010000361         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13839_length_3986_cov_3.989570, whole genome shotgun sequence.
ACCESSION   DAOQJL010000361 DAOQJL010000000
VERSION     DAOQJL010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13839_length_3986_cov_3.989570"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1405)
                     /locus_tag="PLG78_14135"
     CDS             complement(119..1405)
                     /locus_tag="PLG78_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24309.1"
                     /translation="MLQVGAIILSFEEAGDLTALTPWKAVDVWSLRDVLGDLVLGFVP
                     VSTCNRVEFYYTADTDCHSQILDRVISFLPPLANGITPSHQTGRSAARHLIRLASGLE
                     SMVLGETEIRAQLKQAYEDAVKHNTLDRRLRILFQHVFQESRGIRQEVPMSRLPLSVA
                     TIATRHMLRLIAPQTEYLKEASLPHTAVVIGSGPMSRQSAESLSRHFQSIVLVNRTVS
                     KIEPLAARLGASVLSFEEFVAAPERVCEGGKFPATIVTATSRDDAFLTPELVNRILKQ
                     RGGSEKLCIVDMALPADVHPDCATDERVSIITMETIRKELDGNREKRAEAARMASDAI
                     EKALIRTEAAMISGVSSTLLKDIQKEVRDKSRQELDALLGNRLSHLSAKDIRMLYDWA
                     IRAHKEMNRIHRKGLESVLEHYYGSENGAPLAERAE"
     gene            1550..2443
                     /locus_tag="PLG78_14140"
     CDS             1550..2443
                     /locus_tag="PLG78_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE24310.1"
                     /translation="MRYSADAVLIFCTLLWGLTFSLVKESLSETGPLYINVLRFVFSG
                     VLFLPAAILRRRQVRLSTVTWGFGLGVLLFYGFAFQTMGLGTTTAQRSAFLTQMLVIF
                     APIFQLLLFRVPIRAGTIAGIAIVLPGLYFLLSAADVPFADNVGMGDLLGLGCAATYG
                     LYMVLLDRATRHEDVVLIVFFQIVFAAACFVLAFSISDEKMHLQVSTRYITAIAYLSI
                     ATTVLTTYLQSRFQKLVSAARAGVLYSMEPVFASIFAFALLGENPGVRGYAGGGLILF
                     GVILSETWPFFMHRLRRYSPG"
     gene            2650..3132
                     /locus_tag="PLG78_14145"
     CDS             2650..3132
                     /locus_tag="PLG78_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24311.1"
                     /translation="MLQWHQVAGLATWGLWLATNLKGEEAIHSLRRSGEPVANAVLLA
                     DPRNNWPLYYAILNNSEWEAKRGGSTHRTLAGLTAGAYALTAGLALLAPSRPAESDSY
                     DSITAHKWLALLHFAALASLPALGKRIEEGGPKAAKRMQKVGWGGFGALTVAVGVFYF
                     "
     gene            3202..3756
                     /locus_tag="PLG78_14150"
     CDS             3202..3756
                     /locus_tag="PLG78_14150"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNE24312.1"
                     /translation="MKFWLEQSFRIFIIWALVGSTAAAQAQEASSVSFFADHSFKVIE
                     GRCADVAVSGSATKSGSTFTAQNLTIQCSIPKMTTGNRNRDSNMWEMLGYPQVQQITV
                     LVPSASAKAGKAPVSFVVRMHGKEKSMTSDMNFEEQSGSVVVSGDFTVSLAEYDVTPP
                     KLLMLKVGDAVLVRFRIAVPVQGK"
     gene            complement(3725..>3986)
                     /locus_tag="PLG78_14155"
     CDS             complement(3725..>3986)
                     /locus_tag="PLG78_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24313.1"
                     /translation="DRLAYFVIGNPTDGQTDLREAHARRRRRFFSSLFAKSENRFTFS
                     LLTGEGARSGMPSRSSLARSVRGLKTVQVPVTITSPALAQQS"
BASE COUNT          833 a          991 c         1107 g         1055 t
ORIGIN      
        1 ttgatgatga cgagttcagt ttgtggcgcg ttgggagtgg cggcgattct gctctggatg
       61 tagcggctct gtgccagagc gagatgggat cctctggttg caattcgcag ccgcggattc
      121 actccgcgcg ctccgcaaga ggcgctccat tttcacttcc gtagtagtgc tcgagaacgc
      181 tttcgagacc ctttctgtgg atccgattca tttctttatg cgcacgtatg gcccagtcat
      241 agagcatgcg aatgtctttc gcgctcaggt gagagagacg attcccaaga agggcatcca
      301 gttcctgtct ggatttgtcg cgaacttctt tttgaatgtc cttgagcaga gtggaggaga
      361 ctccagagat catggcagct tccgtgcgaa tgagtgcttt ctcgatcgcg tctgaagcca
      421 tgcgtgctgc ttccgcgcgt ttttcgcgat tgccgtcgag ttctttgcgg atggtttcca
      481 tggtgatgat ggacactcgc tcgtcggtcg cgcaatccgg atgaacgtca gctggcaatg
      541 ccatgtctac gatgcagagc ttttcgcttc cgccgcgttg tttgaggatt ctatttacaa
      601 gttcaggtgt gaggaatgca tcatctctcg atgtggctgt tacgatggtt gcagggaatt
      661 ttccgccttc gcagactctt tccggcgccg ccacgaactc ttcaaatgat agaacagaag
      721 cacccagtct cgcagccagt ggttctattt tggaaacagt gcggtttact agaacgatgg
      781 attgaaagtg tcgcgagagc gactctgcgg attggcgact catgggacca gaaccaatga
      841 ccaccgcagt gtgtggcaga ctcgcttcct ttaaatactc ggtctgaggt gcgatcagcc
      901 gcagcatatg gcgcgttgca atcgtggcta cagacaacgg aaggcgactc atgggaactt
      961 cttgccggat gccgcgcgac tcctggaaaa catgctgaaa gagaatgcga agcctgcggt
     1021 ccagagtgtt gtgtttgact gcgtcttcgt atgcctgttt cagctgcgct cgaatctctg
     1081 tttccccgag aaccatggac tccaggccac tggccaatcg gatgagatgt ctggccgcac
     1141 tgcggcctgt ttggtgagaa ggggtgattc cgtttgcgag aggaggaagg aaagaaatga
     1201 cgcgatccag aatctgcgag tgacagtccg tatctgccgt atagtaaaat tcaacacggt
     1261 tgcatgtgga cactggaaca aagcccagaa ccagatcgcc caggacatcg cgcagagacc
     1321 agacgtccac agccttccac ggcgtaagcg cagtgagatc cccggcttct tcaaaactga
     1381 gaatgattgc cccgacctga agcatggctg ttcagtttca tattttatgg cctcagagta
     1441 cttcttttgt catggagttg tcaggggacg ggcaaaccag tcggacttga gacgcagtat
     1501 caagaagcca gaacgcttgc agagggtcct tcttagcaga atttgaccca tgcgctacag
     1561 tgcggacgca gtgctcatct tctgtacgct gctgtggggc cttacgttta gcctggtgaa
     1621 ggaatctctc tccgaaacag gccctcttta catcaatgtg ctgcgatttg ttttttccgg
     1681 ggttttattt ttgcccgcgg ccattcttcg aaggcgtcag gttcgtctgt ccactgttac
     1741 gtggggattt ggcctgggtg tgcttttgtt ttacgggttc gccttccaga cgatgggcct
     1801 tggaaccact actgcacaaa ggtccgcgtt tctgactcaa atgcttgtta tttttgcgcc
     1861 gatctttcag ctacttttgt ttcgcgttcc cattcgcgcc ggaacgatcg caggcattgc
     1921 cattgttttg cctggtctgt actttctact ctctgcggcc gacgttccat ttgcagataa
     1981 cgttgggatg ggcgatctac tgggtctcgg ttgcgctgcc acgtacggtc tgtatatggt
     2041 gctcctcgat cgcgccacca ggcacgaaga cgttgtgctg attgtttttt ttcagatcgt
     2101 ttttgccgcc gcctgcttcg ttctggcttt ctctatttca gacgaaaaga tgcatctgca
     2161 agtttctacc agatacatca ctgctatcgc atatctttcc attgcaacca ccgttctgac
     2221 tacgtacttg cagagtcggt ttcagaaact tgtctcggcg gcgcgtgcag gtgtgctcta
     2281 ttccatggaa ccagtctttg cctcgatctt cgcctttgca ttgttaggtg aaaacccggg
     2341 ggtgcgaggc tatgctggtg gcgggttgat tttatttggc gtgatcttga gcgaaacctg
     2401 gccatttttc atgcaccgcc ttcgcagata ctcccccggg taagaattcg taactggcgc
     2461 ctcaacgggt ggacgatttc gctgaatctc gttctcaact cgaattcata aaaaatcatt
     2521 tttcctttgc ttttgatttg gacgtccgtt ttccttgtac ctatgatccg cagatggttt
     2581 gtccttgttt tcttatatct attcatacag tcgtccattc gcgcagacga attgtccacg
     2641 agacgggaaa tgcttcagtg gcaccaggta gccggcctgg caacgtgggg actctggttg
     2701 gccacgaacc tgaagggaga ggaagccatc cattcgctgc gcagaagcgg ggagccagtg
     2761 gccaatgcgg ttctgctagc ggatcctcgc aataactggc cactttacta tgcaattctc
     2821 aacaattccg agtgggaggc caaaagaggg gggagcactc acaggacact cgcggggctg
     2881 acggccgggg cctacgcgct gacagccggg cttgcacttc ttgccccttc tcgtccggca
     2941 gaatctgatt cctatgacag tatcacagcg cacaaatggc tggcgctctt gcactttgca
     3001 gctcttgcca gtttaccggc gcttgggaaa cggatcgaag aaggtgggcc aaaggcggcc
     3061 aaaagaatgc agaaagtggg atggggtggc tttggagcgt tgacagttgc agtcggtgtt
     3121 ttctatttct agcaacgtcg cagagcagcc atctacggaa gattgaaagt atagtgaaaa
     3181 ttgaaagtac aatgaggcaa tatgaaattc tggcttgagc aatcctttag aatatttata
     3241 atctgggcac tcgtcggttc aacggcggcc gcccaggcgc aggaagcaag ctccgtctct
     3301 ttttttgcgg accacagctt caaagtcatt gaaggaagat gtgcggatgt cgcggtgtcc
     3361 ggttctgcca caaagagcgg cagcacgttt actgcgcaaa acctcaccat ccaatgttct
     3421 attcctaaga tgaccacggg caatcgcaat cgggattcca atatgtggga gatgctgggc
     3481 tatccccagg ttcagcagat cacggttctt gttccgtctg cgtccgcgaa ggcgggaaag
     3541 gctcctgtta gctttgtagt tcgcatgcac ggcaaagaga agagcatgac ttctgacatg
     3601 aattttgaag agcaatcagg cagtgtggtt gtatcgggag atttcactgt gtctcttgcg
     3661 gaatacgacg taactccgcc aaagctgctc atgttgaagg tgggggacgc agtgctggtt
     3721 cgtttcagga ttgctgtgcc agtgcagggg aagtgatcgt aacgggcacc tgaaccgtct
     3781 ttagtccgcg aacacttctg gcgagtgagc tgcgcgatgg catccctgaa cgagcgcctt
     3841 ctcccgtgag tagcgagaaa gtgaatctgt tttcgctttt tgcgaacagc gaagagaaga
     3901 accggcgtct gcgcctggca tgtgcttcgc gcagatctgt ctggccgtcc gttggatttc
     3961 caattacgaa atacgcaagg cgatcg
//
LOCUS       DAOQJL010000362         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13847_length_3984_cov_4.872232, whole genome shotgun sequence.
ACCESSION   DAOQJL010000362 DAOQJL010000000
VERSION     DAOQJL010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13847_length_3984_cov_4.872232"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="PLG78_14160"
     CDS             complement(<1..2058)
                     /locus_tag="PLG78_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24314.1"
                     /translation="MKITRQSIYQAFEQIAVKLDPWILRFLDKSQPFLEKASRGLMRA
                     WLLLTASLSFIWKVFTYPVRAAARASGAIFGTFEYRPSPPVRSILSIKQEGIQFVESR
                     VKVIQKRILNAREARPPVFRTAVMMSAAFALVLGGCSSFQLFKKLTRTNATVSGPEYV
                     KPSDETSPSSVSIHFSNPAVRIDLIGKTADIQMKPSHPGTWTFVDDSTITFVPSQHWP
                     AEKSYSVTLKPEFFSPSIKLDDYQVGFETAPFTVTVASAQFYIDPKDPRIKKAVITLD
                     FSHPVDAKSISDRVSFEYKDGLLSGKPLANSVTLDATEMRAFVHSDIVSIQEKEASLV
                     FNLKSGIHAKDGGGSSDSVHADVPVPGKYTHFFIDSTAVQLVRNESYELDQVLFVETT
                     ASITPEELAKNITAYELPKNRPASPGTSESKNHQWQVKEIDEDTLAHSRKLQFVRADE
                     NTEEGTVHPYKIQAQEGAQVFVRVGKNTRAVGDFLLKEKYESIVRVPDFQREARIMHD
                     GAILSLSGEKRISVLSYDNDALEFEIGRIMPDQINHLISQSYGNFNSPYFSNYNFNED
                     NISERYTETIQLNREKGKRLQYASFDFSKYLSAKGPRLQNGLFYLKVRGVRKKKQDQE
                     TENEGESDEVRIGKLEDKRLVLVSDLGILVKAARSGTRDVFVQSFAQGFPVCSRQDPV
                     ICKA"
     gene            complement(2099..2773)
                     /locus_tag="PLG78_14165"
     CDS             complement(2099..2773)
                     /locus_tag="PLG78_14165"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE24315.1"
                     /translation="MKEKRAKKTQARSQETRETIYEAALVLFQKKGFEDATMREIAAS
                     AGVALGSAYYYFRTKEEVVLEFYKRTLEKDDQIKAYFKEETGLKERITYVIRLRIKQF
                     APFKKFLWVLAKNAVVPGSPLSPFSEESAEIRDKAIAIFEEAVADCKPAPAQLAKELP
                     FLLWLYQLGILLFWVLDQGKTMRKTDILLNTTSEMIVWLVKSSGLPIFGGISKRISTL
                     IQELRD"
     gene            complement(2770..3579)
                     /locus_tag="PLG78_14170"
     CDS             complement(2770..3579)
                     /locus_tag="PLG78_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE24316.1"
                     /translation="MKIGTLLLALAFAMPVGAQTLHDMAIQGQEDDLARKVTAKNIDS
                     LDREEKTMLMVAAENGQTGVVRMLVRAGANPAIQDRAGNTALHYAIIRGDEEITRLLA
                     TRGKVLEIQNGNGHRPIDLAQSQSNDRIVEILRASGSMPPHVWSKLTLLLYFLYLIIS
                     LGLTVWVARTLSKNGRIFLVDAFHANEPLADSVNHLLVVGFYLINIGYVTLTLREKIK
                     PIAPEEMVESLSVKVGLVLLILGTMHFFNMFVFSRMRKRTQQKPALQEKRA"
BASE COUNT          932 a          962 c          993 g         1097 t
ORIGIN      
        1 agccttgcag atcaccggat cctgccggga gcagaccgga aagccctgcg caaacgattg
       61 aacaaatacg tcgcgagtgc cagagcgagc tgctttgacc agaattccta aatccgaaac
      121 cagaaccagg cgcttgtctt cgagtttacc tatccgcact tcatccgatt cgccttcgtt
      181 ttccgtttct tgatcctgtt tcttcttacg cactccgcgc acttttagat agaagagccc
      241 attctgaaga cgcgggcctt ttgcagaaag atactttgaa aaatcaaacg aagcatactg
      301 cagtcgtttt cctttctcac ggttgagctg gatcgtttcc gtataacgtt cggatatatt
      361 gtcttcattg aaattgtaat tggaaaagta gggactgttg aagtttccgt aggattgcga
      421 aatcaaatga ttgatctgat ccggcatgat cctgccgatt tcaaattcaa gagcatcgtt
      481 gtcataggat agaacagaaa tgcgtttctc acctgaaaga ctcagaatgg ctccgtcgtg
      541 catgatgcgc gcttctcttt gaaagtcagg cacacgcacg atcgattcgt acttctcttt
      601 caacaggaag tcgcctacgg cgcgggtgtt cttgcccacc ctgacaaaga cttgagctcc
      661 ttcctgtgcc tggatcttgt aaggatgaac ggtaccttct tctgtgtttt cgtctgctcg
      721 cacgaattgc agtttacgcg aatgggccag agtatcctca tctatctcct ttacctgcca
      781 ctgatggttc ttgctttccg atgtgcccgg agatgcaggg cgatttttgg gaagctcgta
      841 tgcggtgatg ttttttgcga gttcttctgg agtgatggaa gcagtggttt ccacaaagag
      901 cacctgatcg agttcgtagg attcgtttcg cacaagctga acggctgtgc tgtctatgaa
      961 gaagtgagtg tactttccag gcacaggcac atctgcatgc acactgtcag aactgccccc
     1021 gccgtctttc gcatgaatgc cggacttcag gttgaatacc agacttgcct ctttttcttg
     1081 aatggacaca atatctgagt gaacgaaagc gcgcatttct gtggcatcca gagtgactga
     1141 gtttgccaga ggcttgccgc tcaataggcc gtctttgtat tcgaaggaaa cacgatcgct
     1201 gattgatttt gcatctactg gatgagagaa gtcgagcgtg atgactgctt tcttgatgcg
     1261 aggatctttc ggatcgatgt agaattgcgc cgaagcaaca gtcactgtaa agggcgccgt
     1321 ttcaaatcca acctgatagt catcaagttt gatggaaggg gaaaagaatt caggcttgag
     1381 agtgacagag tacgattttt ctgcgggcca gtgttgtgac ggaacaaatg tgatggtgga
     1441 atcgtctacg aacgtccagg ttcccgggtg agatggcttc atctggatgt ctgcggtttt
     1501 tccaatcaga tcaattctta cagcagggtt gctgaagtga atgcttacgg aagagggaga
     1561 tgtttcatcc gaaggcttta cgtattccgg accgcttacg gttgcatttg tgcgagtgag
     1621 tttcttgaag agctgaaagg aactgcaccc tccaagaacc agcgcaaatg cagcggacat
     1681 catgactgcc gtgcgaaaca ctggaggcct ggcctctctt gcgtttagaa tgcgcttctg
     1741 gatcactttc actctggatt ccacgaattg gattccttcc tgcttgatcg aaaggatgct
     1801 tcgaaccgga ggcgaaggtc tgtactcaaa cgttccaaag atggcgcccg aagcacgagc
     1861 ggcggcgcgc actggataag tgaaaacctt ccagatgaaa gagagcgatg ccgttaacag
     1921 gagccaggcg cgcatcaggc ctctggatgc tttctccaaa aatggttgtg atttatcaag
     1981 gaatcgaagg atccacggat ccagtttcac tgcgatttgt tcgaatgcct ggtagatgct
     2041 ctggcgggtg attttcacgg cctggtgtga ggaatcgtgg aggcctgtgc aagcaaaatt
     2101 agtcccgcag ctcctgaatc aaagtggata ttcgttttga gattcctcca aagataggca
     2161 ggccagaaga tttcaccagc catacgatca tctcagaagt ggtgttcagt aaaatgtccg
     2221 ttttgcgcat tgtttttccc tgatccagca cccagaagag aaggattcca agctggtaga
     2281 gccagagaag gaaggggagt tcttttgcca gttgcgctgg cgcgggctta caatctgcca
     2341 ctgcctcttc aaagatcgca attgccttgt ctcgaatttc ggctgattct tcactgaaag
     2401 gagagagtgg acttcctgga acgactgcat tttttgcaag cacccacagg aactttttga
     2461 acggagcgaa ttgtttgatg cgaaggcgaa tcacatatgt gattctttct ttcaggcccg
     2521 tctcttcttt gaagtaagct ttgatttgat cgtctttttc cagagtgcgc ttgtagaatt
     2581 ctagaaccac ttcttctttg gtgcgaaaat agtagtacgc agatccaagc gccacgcctg
     2641 cactcgccgc aatttcgcgc atggttgcat cttcgaagcc tttcttttga aagaggacca
     2701 gcgcggcctc atagatggtc tctcttgttt cctgagaccg cgcctgggtt ttctttgctc
     2761 ttttttcttt catgcccgct tttcttgaag tgctggtttt tgctgggtgc gcttgcgcat
     2821 tctgctgaat acaaacatat tgaagaaatg catggtaccc agaatcagga gcaccagacc
     2881 tactttcacg ctcaacgatt caaccatttc ttccggcgcg attggtttga ttttttctct
     2941 gagagtcaga gtcacgtatc caatgttgat cagatagaat ccaaccacca ggagatgatt
     3001 cacagagtct gcgagcggtt cattcgcgtg aaacgcgtcc acgagaaaga tccttccgtt
     3061 ttttgaaaga gtgcgcgcca cccagacagt cagacccagg ctgatgatca gatacagaaa
     3121 gtaaagcaac aacgtcagct tgctccacac atgcggtggc atactcccac ttgctctaag
     3181 aatttcgacg attcgatcgt tgctctggct ctgagccaga tcgatcggcc gatgaccatt
     3241 tccattttgg atctcaagca cttttccccg agtggccagg agccgggtga tctcctcatc
     3301 tccgcgtatt atcgcgtagt ggagtgccgt gtttccagcg cggtcctgga tcgcaggatt
     3361 tgcaccggca cgcacaagca ttcgcaccac tccggtctgg ccattttcag ccgcaaccat
     3421 cagcattgtc ttttcttccc ggtccaggga gtctatgttc ttcgccgtca ccttgcgcgc
     3481 cagatcgtct tcctggccct gaatggccat atcatgcagc gtctgggcgc ccacgggcat
     3541 ggcaaatgcc agggcaagaa gtagggttcc gattttcata actgcctcca ataattgaac
     3601 atgttcaaaa tagacgatag ggagttcttg tgtcgatata aaaaatttga acgtgttcaa
     3661 aaattctgat cagcatgtgc gtggccgcag accttcagca ccagccaggg gctgtcacag
     3721 aggtttgggc ttcttcattt ctgtgcgaag ctgagaccgg tcgtgctgtt tattaccgaa
     3781 aacatgtagt tcattccgga tttcctctca atactacatt ttatcaagtt tctggttgac
     3841 gattgctttc gcagtctcag ccagagccta tgagaaatag caaaaagaac cgggtattca
     3901 aattcactgc tactgttgta ggagccatca tcctttcttt cagtgtcctg gcttgcggcg
     3961 ggaagtccaa caaatgctcg tctt
//
LOCUS       DAOQJL010000363         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13849_length_3984_cov_4.373123, whole genome shotgun sequence.
ACCESSION   DAOQJL010000363 DAOQJL010000000
VERSION     DAOQJL010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13849_length_3984_cov_4.373123"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLG78_14175"
     CDS             complement(<1..511)
                     /locus_tag="PLG78_14175"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNE24317.1"
                     /translation="MAEKKPRKSYECTSCGAKSSRWEGKCGTCGEWNTMVESELQPGL
                     VPGKRAAQGHASFESLSEANADIARITTGIQDLDNVLGGGLVPGALVLLGGEPGVGKS
                     TLLLEIARRFEGKFLYFSGEESPGQIRIRATRLGVMSSNVQVSRETDLETIAQEIEKH
                     KPDIAVIDSI"
     gene            complement(504..1850)
                     /gene="dnaB"
                     /locus_tag="PLG78_14180"
     CDS             complement(504..1850)
                     /gene="dnaB"
                     /locus_tag="PLG78_14180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNE24318.1"
                     /translation="MPWDENAERQVLGTLLVRPDLFLQIGTTVAADDFYPEGHKVIYR
                     AIVEMTQKGEAIDPLRVSQYLTDRSLIAQAGGAETLLQIARDALPSGLIFQHARRVHS
                     FGIRRALIKAAREIQEESVSPQEDESVYLRTIEDKILSITQQTSSTDVAPIGEFRDSF
                     GQHVQHLIQARGGLSGLRTNFREFDRMTSGLKGGELIILAARPSAGKTTLAMNIAANV
                     AIREKKAVLVFSLEMSANQLLGRLMCAESGLNHSDYQRGQMHGKEQTFLAAMNRVFDA
                     PMHLCESGYLELLEAIALTRKLHLQLKGEGKSLGLIVVDYLQLLNDSAARRQSRQLEV
                     ASISRALKQLSKQVDAPVLALSQMNRSVESRRGDGGRPQLSDLRESGAIEQDADIVMF
                     IHRELNPDPNDPEAMENRGKAEIILAKHRNGPVGSFRLAFRPEMNRFDDLDPEYNG"
     gene            complement(1869..2429)
                     /gene="rplI"
                     /locus_tag="PLG78_14185"
     CDS             complement(1869..2429)
                     /gene="rplI"
                     /locus_tag="PLG78_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNE24319.1"
                     /translation="MKVILQKDVPGLGDAGEVKNVADGYARNYLLPRKLGTAARGDST
                     KVLEHQKRMMQRKIDKRNKEMADVAEKLKALGTVSISVRVGAKKKLFGSVTPQAVANA
                     LKDLGYPVDKRKVELTDAVRTLGNFKFRVRLAEKVIVPMALEVLADKDSKVEEEEYVP
                     PAVPQTEAAAPEAAAEAKPEKSKKSK"
     gene            complement(2454..2903)
                     /gene="ssb"
                     /locus_tag="PLG78_14190"
     CDS             complement(2454..2903)
                     /gene="ssb"
                     /locus_tag="PLG78_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE24320.1"
                     /translation="MASDLNRVVLVGRLTRDPELKSVGSGSTLCKFSLASNRTYTAGG
                     EKREEVSFFNCTAWGKQAEILSQYARKGKQLAIEGRLQQRSWDGPDGKKQSAVDIVVE
                     NFQFLGAAGGRDDSSRGGGEYSPQPAGVRQEDAGFASGDYPDDDIPF"
     gene            complement(2893..3174)
                     /gene="rpsF"
                     /locus_tag="PLG78_14195"
     CDS             complement(2893..3174)
                     /gene="rpsF"
                     /locus_tag="PLG78_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE24321.1"
                     /translation="MRNYHIIVVIKDAPDTEELKKGVIQLLGRHEAKVSEDQKWGSRR
                     LPHGMQHEHVGIYYCLTAQMDAGQVKELSHDLNIQSGVLRFMVSRADGK"
     gene            complement(3691..>3984)
                     /locus_tag="PLG78_14200"
     CDS             complement(3691..>3984)
                     /locus_tag="PLG78_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24322.1"
                     /translation="NSKLTVEFYEAPSTVVPKWEGPDGVPDGAPDWKPLSTLELNTNV
                     KDPIPYPKQAQIVLNYKKLLRRVDRLLARVQKMKILDRNTALEKVIDYNGSNY"
BASE COUNT          867 a         1066 c         1001 g         1050 t
ORIGIN      
        1 ggatggaatc aatgactgca atgtccggct tatgcttttc tatttcctga gcaattgtct
       61 caaggtccgt ttcgcgcgaa acttgaacgt tagacgacat gactccaaga cgagtggccc
      121 ggatgcgaat ctgccctgga gattcctccc ctgaaaaata gagaaactta ccctcaaagc
      181 gtcttgcgat ttcaagaagg agtgtgctct tccctacacc gggttcccct cccagaagaa
      241 cgagagctcc aggcacaagc ccgccgccga gtacgttgtc caggtcctgg atgcccgttg
      301 tgattcttgc gatgtctgca tttgcttctg aaagagattc gaaactggca tgcccttgag
      361 ccgcgcgctt ccccggcacc agaccaggct gaagctctga ctctaccata gtattccatt
      421 caccgcatgt gccgcatttt ccttcccagc gagaagactt cgcgccacag gaggtgcatt
      481 cgtaagattt gcgcggcttt ttttcagcca ttgtattccg gatcgaggtc atcaaatcgg
      541 ttcatttcag gcctgaacgc gaggcgaaaa cttcctacag gaccgtttct gtgcttggcc
      601 agaatgattt ccgctttgcc gcgattttcc atggcttctg gatcatttgg atccggattg
      661 agttccctgt ggatgaacat tacaatatct gcatcctgtt caatggcgcc tgattcgcga
      721 aggtcagaaa gctgaggcct tcccccgtct ccgcgtctgg attcaacgga tctgttcatc
      781 tgggagagag caagcacagg cgcatccacc tgtttggaca gctgtttgag agcgcgagaa
      841 atggacgcaa cttcgagctg tctgctctgt ctgcgtgcag cagaatcgtt gagcaattgt
      901 aagtaatcta caactatcaa accaagcgat tttccttcgc ccttgagctg gagatgaagc
      961 tttctcgtga gtgcaatcgc ttcgagtagt tccagataac cggattcgca caaatgcatg
     1021 ggtgcatcga atactcggtt catggcagca agaaacgtct gttcttttcc gtgcatctga
     1081 ccgcgctggt agtcgctgtg attgagaccg gactcagcac acatgagtct ccccaggagc
     1141 tgattggcac tcatctccag agaaaaaaca agcactgctt ttttttcgcg gatggcaacg
     1201 ttagccgcta tgttcatggc cagagtcgtt ttacctgcag aaggacgcgc ggcgagaatg
     1261 atgagttctc cgcctttcag gccggaagtc atgcgatcaa attcgcggaa gttcgtgcga
     1321 aggccggaga gaccaccgcg ggcctgaatg aggtgctgca catgctgtcc aaaactgtcc
     1381 cggaattctc cgatgggtgc aacatctgtg gacgacgtct gctgggtgat ggaaagaatc
     1441 ttatcttcta tggtgcgaag ataaacggat tcgtcttcct gcggagaaac ggactcttcc
     1501 tggatttcgc gcgcggcttt gatcagtgcg cgcctgattc caaacgaatg cactctgcgc
     1561 gcatgctgga agatcaaacc ggaagggagc gcatcgcgcg caatctgaag aagagtttcc
     1621 gcgccgcctg cctgtgcgat gagacttcgg tctgtgaggt attgagatac acggagaggg
     1681 tcgattgcct ctcccttctg agtcatctct acgatggctc tatagataac tttgtgacct
     1741 tctggataga agtcgtctgc ggcaactgtg gtgcctattt gcaggaagag atccggccgc
     1801 acgagaagcg tgccgagcac ctggcgctcg gcattctcat cccacggcac gctcacagga
     1861 ttgacctatt acttggactt tttagacttt tctggctttg cttctgcagc tgcttccgga
     1921 gctgctgctt ctgtctgtgg aactgcaggc ggaacatatt cttcttcttc cactttgctg
     1981 tccttgtctg caagaacttc aagagccatt ggaacgatta ctttttcagc caggcgaacg
     2041 cggaatttga aattccccag tgtgcgaact gcgtctgtga gttctacttt gcgtttgtct
     2101 accgggtaac ccagatcttt gagagcgttt gcaactgctt gcggagttac agatccgaag
     2161 agtttctttt ttgcaccaac gcgcactgaa atggaaactg ttccaagagc cttgagtttt
     2221 tcggctacgt ctgccatctc tttgttgcgc ttgtcgatct tacgctgcat cattcttttc
     2281 tgatgctcga gtactttggt tgaatcgccg cgtgctgcag ttcccagttt gcggggaaga
     2341 aggtaattgc gagcgtaacc gtccgcaacg ttctttactt caccggcatc gcccaggccc
     2401 ggcacatctt tttgaagaat tactttcatc tcatgctccc tttactcttt caatcaaaac
     2461 ggaatatcat cgtctggata atcacccgaa gcgaagcctg cgtcttcctg tctgactccc
     2521 gcaggttgag gcgaatactc tccgcctcct ctgcttgaat cgtcacgacc gcctgctgcg
     2581 ccaaggaact ggaaattttc tactacgata tctaccgcag attgtttctt tccgtccgga
     2641 ccgtcccagc ttctctgctg caacctgcct tcaatggcga gctgcttgcc ctttctggca
     2701 tactgactga gaatctcagc ctgcttgccc caggcagtac agttaaaaaa acttacttct
     2761 tccctcttct ctccgcctgc agtgtatgta cgattgcttg ctagagaaaa tttgcaaagc
     2821 gtgctaccag aaccaacaga cttgagttct ggatcacgcg tgagtcgccc gacaaggacg
     2881 actcgattga gatcacttgc catccgctct tgagaccatg aatcgcagaa cgcctgactg
     2941 gatgttcaga tcgtgagaca attccttcac ctgacctgca tccatctgag ccgtaaggca
     3001 atagtagatt ccaacgtgct cgtgctgcat tccgtgaggc aaacgtctag aaccccactt
     3061 ttgatcttcg cttacttttg cttcgtgacg gcccagaagt tgaatgacgc ccttcttgag
     3121 ctcttctgta tcaggcgcat ccttgatcac gactataata tgataatttc tcataggtac
     3181 cgggcaattt ccgggacggc ctggacctgt caagaaattg gtttttagag ggcctggaag
     3241 aggcaaaaag ggccgagttc gacccggccg aaggcttcgg ccgtggatcc ggtcgtggct
     3301 gtccgaccag ggcgaccctt ccccggcgcc cgcggttcta catttcgagg cgacccgagg
     3361 ccaaacgggt gccgcgcgcc cgtcggtttt atcacgcaat agaagtgcgt attacatacg
     3421 aatgcaggca ttaacacgaa cgttttgttc gttttacatc ggggggaaga tatgagggcg
     3481 aacgggtgcc ggcgcggccc ggatttatca cgcaataaaa gtgcgtatta catacgaacg
     3541 cacgcattaa cacgcacgtt ttgttcgttt tacatccggg ggaagatatc gcaaaacgtt
     3601 aaatttctct caaggcccgc tgctgttccg cgaacaacgc cgcataacgg tagagcaaat
     3661 ccacccactg cggatttaac gcccctcgcc tcagtaattg gagccgttgt agtcgatgac
     3721 cttttccagc gcagtgttgc gatcgagtat cttcatcttc tgcacgcgag ccagtaatct
     3781 gtccactcgc ctcagcagtt tcttgtagtt caagacgatc tgagcttgct ttggatacgg
     3841 aatcggatct ttcacatttg tattgagttc gagagtagac agaggcttcc agtctggcgc
     3901 gccatccggc actccgtctg ggccttccca tttgggaacc acggtagacg gagcttcgta
     3961 gaactctact gtaagtttgc tgtt
//
LOCUS       DAOQJL010000364         3979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13880_length_3979_cov_4.296126, whole genome shotgun sequence.
ACCESSION   DAOQJL010000364 DAOQJL010000000
VERSION     DAOQJL010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13880_length_3979_cov_4.296126"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..978
                     /locus_tag="PLG78_14205"
     CDS             85..978
                     /locus_tag="PLG78_14205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE24323.1"
                     /translation="MNDSPSPPSHSRAFLGFLFLIAIVSLGHASLFARAAQAPPEVVG
                     FWRMSGAALILFPFALRYANVNRKQILLSFIAGFFFFIHLWTFIFAAQHTKIANCVVL
                     FAVNPVFTSIGHWFLDGVRPRWKTLFAYAAAGAGVLIALHNKTSFAAFSLQGDLLAVV
                     SAGFFSAFILTGNAARKNLNNLVFSPIVYFSAAVCFLVCAVLRSIPLLPWPLESWAAI
                     LGYILVPTLLGHTLYTYLLSHVRIHVMSFGKMIEPLVAIVSAWFLFSEKPGEEILLSF
                     VFTAVATLLILWPDQNDPRRV"
     gene            959..1330
                     /locus_tag="PLG78_14210"
     CDS             959..1330
                     /locus_tag="PLG78_14210"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNE24324.1"
                     /translation="MTLEEFEQIVEEERLALPREFADLLDLHNVAIMVDDWPLPEILR
                     TAGIRNPSSLFGFYSGIPLPDRGADKPLLPDRIWIFREPILRAVSTDAQIRAEIRTTL
                     RHEIAHFFGFSDEHLLREGTY"
     gene            1496..1930
                     /locus_tag="PLG78_14215"
     CDS             1496..1930
                     /locus_tag="PLG78_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNE24325.1"
                     /translation="MNRAIKALKYAVTVGLFFAGASLFAQASPVGSWKTIDDETGKPK
                     SIVNIWEQGGVLYATIADLINPDEPDPKCTKCTGDYKDKPIKGMTIMWGMKKDGDSWS
                     GGQILDPKKGQTYSCKLWLEGSNLKLRGYLGPFYRTQTWYRN"
     gene            2001..3242
                     /gene="tilS"
                     /locus_tag="PLG78_14220"
     CDS             2001..3242
                     /gene="tilS"
                     /locus_tag="PLG78_14220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNE24326.1"
                     /translation="MRDLLDRLQQVLKFAPPGIPILCVSGGQDSMLLLHAYRSLFESG
                     IGLPEPVVFHLDHRLREDSGLDADFVIRTAREAGFSVYLESRNVGEFSRRSKVSLEEA
                     GRVLRYRTLARLVDRIGSAYAVTAHHADDYLESVLIHLIRGGGASALSTLPLVAGIHG
                     VTVFRPFLSVRKSEIERYATSFEFREDSSNKSQEFLRNRIRAHIAPVLLGEGLDPVRL
                     WQNFHAELWPAGIRVPAHHVRVDRSLVCGASLADIKQQLDLATRSLGIPPVSRSLALS
                     LYNASRKESFRFRFRTNELQAWCDERSPLHIFRSDSPLLSPPQMTPQVDGVSILYGGK
                     TRFIALRTGERARMFEPGLRVRMGAGHKKLKKVFQEEGVPSPVRPFVPLLVDQAGFVI
                     RAGLSFLGQKDLFFSGAQGKN"
     gene            3274..>3979
                     /locus_tag="PLG78_14225"
     CDS             3274..>3979
                     /locus_tag="PLG78_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE24327.1"
                     /translation="MPFRRFFESPRPLEFLGKDPEYWLLRGFTHACLDWMRDYTHLDT
                     EGLENIPSHGGALIIPNHSGVMGWDAFVIQNEILKAGHRIPRTMSHNFWHQSPLFKRW
                     SFKLGYFPQDFKMAVRILRKNKVMLLFPEAEAGNFKPSTQMYQLQPFNPGFISLSIIT
                     GKPIVPACIIGAEETHLNLGTMDWTEKYIGAKVPIPLNLIPLPVQWKIIFLKPVSLEK
                     YGKREAKNIRFLEEAAG"
BASE COUNT          882 a         1008 c         1025 g         1064 t
ORIGIN      
        1 ccagcataac atggcgaaaa cggaaaaccc ttgatttata tcaagtattg aatttttttg
       61 tcgccaggtg ctgcgaaagt tattatgaac gattcacctt cgccaccttc gcactcgcgt
      121 gcgttccttg gatttctatt tctaatcgcc attgtttctc tggggcatgc ttctttgttt
      181 gcgcgcgcag cacaggctcc tccagaggtg gttggtttct ggaggatgag tggcgcggcc
      241 ctgatccttt ttccatttgc gcttcgctat gcgaacgtga atcgcaaaca gatccttctc
      301 tcctttatcg caggattctt cttttttatt cacctatgga cgttcatatt tgcggcacag
      361 catactaaga ttgcgaattg tgtggttcta tttgcggtca atcctgtgtt cacgtcgatt
      421 ggccactggt tcctggacgg agttcgccca cgctggaaaa ctttgttcgc ctacgcagcc
      481 gcaggtgccg gggttctgat tgctcttcac aacaagactt cgtttgcggc gttttcgctt
      541 cagggcgatt tgcttgccgt ggtatccgct ggattttttt cagcgttcat tctaacgggc
      601 aacgctgcca ggaagaacct gaacaatctg gtgttttcac ccattgtgta tttttcggcg
      661 gcggtatgct ttcttgtgtg cgccgtcctg cgatctattc cgctcttgcc ctggcctctg
      721 gaatcatggg ctgcgattct gggatacatc cttgtgccga ccttgctcgg acacactctc
      781 tacacctatt tgctatctca cgttcgcatt cacgtgatga gttttggaaa aatgatcgaa
      841 cctctcgttg caattgtttc tgcgtggttc ttattttctg agaagccggg ggaggagatc
      901 ctactttcct ttgtgtttac ggctgtggcc acattgctga ttttgtggcc ggatcagaat
      961 gaccctcgaa gagtttgaac agattgtaga agaagagaga ctcgcccttc cgcgagaatt
     1021 cgcagatttg ctcgatttgc acaatgtggc tatcatggtg gatgactggc ctttgcctga
     1081 gatcttacgc accgccggga ttagaaatcc atccagtctt tttggcttct attcagggat
     1141 tcctctcccg gatcgagggg cggacaagcc tcttttgccg gatcgaatct ggatctttcg
     1201 cgagccgatt ttgcgggcag tctccacaga tgcgcaaatt cgcgccgaga ttcgcacaac
     1261 actgagacat gagattgccc acttctttgg cttcagcgac gagcacctac ttcgcgaggg
     1321 tacatactga ttccagtgtc tgacgcaccc gctgacccac cgcagtgcag gaaagttcac
     1381 ggaaaatcac cttcctggga accaggaaaa ttcttgttgc ctccccggat acagaaccta
     1441 atgacaattc gccgcgtcca aagcggtgtg tacgtaaaaa aaaatggagg gtgtcatgaa
     1501 ccgagctatc aaagcgctca agtatgcggt cacagttgga ctgtttttcg caggtgcaag
     1561 tctgttcgcg caagcgagtc cagtcggcag ctggaagaca attgacgacg agacaggaaa
     1621 gccaaaatca attgtaaata tctgggagca gggcggcgta ctgtacgcta cgattgcaga
     1681 tctgatcaat ccggatgaac cggatccaaa atgcaccaag tgcacaggag actacaaaga
     1741 caagccaatc aaaggcatga ccattatgtg gggcatgaag aaagatggcg attcctggtc
     1801 aggtggtcag atccttgatc ctaaaaaagg ccagacatac agctgcaagc tgtggctgga
     1861 aggatccaat ctcaagcttc gcggttacct tggaccattc taccgcacac aaacctggta
     1921 tcgtaactaa tatcaggttc aaaagccgcg cggcgtaagc ccgcggctga tttctaaatt
     1981 ttctcaacta acggcttgat gtgcgggatc tactcgatcg tttgcaacaa gtcctgaaat
     2041 tcgctcctcc gggaattcca attctctgcg tttcaggtgg ccaggattca atgctgctcc
     2101 ttcacgcata ccgctctctt ttcgaatcgg gcattggatt gccagagcct gttgtgtttc
     2161 atctcgatca tcgcctgcgt gaggattcgg ggctggacgc ggattttgtg attcgcactg
     2221 cgcgcgaggc cgggttttcc gtttatcttg aatcgcgcaa tgttggcgaa ttttccagac
     2281 gctcaaaagt cagcctcgaa gaagcgggtc gcgttcttcg ctatcgcacc ctggcaaggc
     2341 tggtggacag aattggaagc gcgtatgcgg ttaccgcaca ccacgcagat gactatctgg
     2401 aaagtgttct cattcatttg attcgagggg gtggagcgtc tgcactttct acacttcctc
     2461 tggttgcggg cattcacgga gtgacggtgt ttcgcccatt tctgtctgtg cgcaaatcgg
     2521 aaattgaacg ttatgcgacc agcttcgagt ttcgagagga ctcttcaaat aagagccagg
     2581 agtttctcag aaatagaatt cgcgcacaca ttgcgcctgt gcttctaggc gaaggtcttg
     2641 acccggtgcg cctctggcaa aattttcatg cagagctgtg gccggcggga attcgtgttc
     2701 ccgcgcatca tgtgcgggtg gaccggtctc ttgtgtgtgg agcctctctc gcagacataa
     2761 agcagcagct cgatctggca actcgcagtc ttggaattcc gccagtttcg cgctctcttg
     2821 cgctctctct gtacaacgcc agtcggaaag aatcgtttcg ctttcgcttt cgcacaaatg
     2881 aactgcaagc ctggtgcgat gagcgcagcc cacttcacat ctttcgctcg gattcgcctt
     2941 tattgagtcc tccgcagatg accccgcagg tggacggagt gtcgattttg tacggcggaa
     3001 agactcgttt cattgctttg cggaccgggg agagggcgcg aatgtttgag ccggggctca
     3061 gagtccgcat gggagccggg cacaagaagc tcaagaaagt gttccaggag gagggcgttc
     3121 cctcacctgt gcgccccttt gttccgctcc ttgtagatca ggccggattt gtgattcgag
     3181 cgggtctttc ttttctggga caaaaggatt tgtttttttc cggcgctcag ggaaagaatt
     3241 gacgaggtga tcccatagtg cacatccttt gccgtgccgt ttcgtcgctt ttttgaatcg
     3301 cctcggcccc tggaatttct cggcaaagat ccggaatact ggctactacg cggattcact
     3361 cacgcctgcc tggactggat gcgggactac acacatctgg atacagaagg tctggagaac
     3421 attccttcgc atggtggggc tttgatcatt cccaatcaca gcggagtgat gggatgggat
     3481 gcattcgtaa ttcaaaatga aatcctaaag gcgggacata gaattccgcg cacgatgagt
     3541 cacaatttct ggcaccagag tcctctgttc aagcgctgga gtttcaagct cggctatttt
     3601 ccgcaagatt tcaaaatggc agttcgcatt cttcgaaaga acaaagtgat gcttttgttt
     3661 ccagaagcag aagcggggaa tttcaaaccg tccacgcaga tgtatcaact gcaaccattc
     3721 aatcccggct tcatttcgct ttccatcatt actggaaaac caattgtccc ggcatgcatc
     3781 atcggcgcgg aagaaacgca tttgaatctt ggcaccatgg actggactga aaaatacatt
     3841 ggtgcaaaag ttccgatccc gctgaacctc attcctctcc ccgtgcaatg gaagattatt
     3901 ttcctgaagc ccgtatccct tgagaagtac ggcaagcgag aagcaaaaaa catccgcttc
     3961 ctggaagaag cggcaggca
//
LOCUS       DAOQJL010000365         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13948_length_3965_cov_4.836829, whole genome shotgun sequence.
ACCESSION   DAOQJL010000365 DAOQJL010000000
VERSION     DAOQJL010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_3965_cov_4.836829"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLG78_14230"
     CDS             complement(<1..265)
                     /locus_tag="PLG78_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24328.1"
                     /translation="MRFRSLMVALIIPVAVVVCDRLLFDRVLFSLSNPSGWDSFRWYN
                     FESVFRDVERRRSSDTRPLIVAVGSSIAQYSILPREMENHLGGK"
     gene            complement(262..1383)
                     /locus_tag="PLG78_14235"
     CDS             complement(262..1383)
                     /locus_tag="PLG78_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24329.1"
                     /translation="MNLIRKALVRSLPLSMRVKSALQENGCRVRFDFESRDWSSDISS
                     FLFDDGSALRSADVTNAPRDPIKTLSETGYFSKTELLYLEAVENDPDDAGRITLYWPA
                     ERTSLERWIAGSSRVHPSALLPAKEAGKIESIEMPSESAEPGISAPATEVTQTAPSDP
                     ITDQKDPLRIADEKDRKKPAPPAREAQVPSRPVLTPWGTMRYGVVGGLRGTTFRRSYI
                     LHRTDRMCFAMMAGEKSAVQTSQGPFIEYLKKMDDWPLAHQSYSQKHPQQMETISIEI
                     FSDGHCRFFCDGIQAVYWSGRLKKLMRFPNTQAPLKSRLHSGDFVLLIPGGWNSREAA
                     EMREIFLYQESDLAERLSALLDYSARGKGVLLQGPEGVP"
     gene            complement(1385..1831)
                     /locus_tag="PLG78_14240"
     CDS             complement(1385..1831)
                     /locus_tag="PLG78_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE24330.1"
                     /translation="MQETAHDYDLMKLGFEGERAITADLFFKYGRTYKPKEIILKEGD
                     KGSEVYLIISGKVVVTERIEKGSYRVLNALGPGEIFGEMAMLENAPRSATLIAAVDTK
                     LLCLTPENFEKIFKTHPRWAFKILVALGRRIQAAFTTVEQHFGVRK"
     gene            complement(1833..2240)
                     /locus_tag="PLG78_14245"
     CDS             complement(1833..2240)
                     /locus_tag="PLG78_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE24331.1"
                     /translation="MDHQTYDRPSLETIKGIGRPMTFEAGQKIYQDAYLVGDPCAYLI
                     VEGEVELVKAYTPLQKDVFRFGKGDVFGMLEVYVNTARMTDARAATQVQVIGFSRVEF
                     ERAMAANLTFALTCIRILSKMLRQINLKLKQMP"
     gene            2302..3237
                     /locus_tag="PLG78_14250"
     CDS             2302..3237
                     /locus_tag="PLG78_14250"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNE24332.1"
                     /translation="MPVTFDECFQTFPAINPPRDFAGFWKSALESLRRVPVDPRQRMV
                     IKRTLVREQITEVRFKSIGRYELRGQLHVPRKRGRVPGVISFHDYQDNRILDTDKAIG
                     DAGLAHLRMDLRGHEAIVPGMPFTPVKDAKLDPPSSSYPYLAVLDALRAVDYLRIHES
                     IDPNKIAVIGRGFGGSLAAFAAALYPDKIHAMSLERPSFLYLTRWLGESTSVLAQEVR
                     EILGPSRGKTKARKALDFLDCLYFAPALKQPAFFTVGLADDKSSPACAFALFNHLQSD
                     KTMDLYPDEEKDPLGTGQRKKSAEFLSSVFESNAG"
     gene            complement(3241..>3965)
                     /locus_tag="PLG78_14255"
     CDS             complement(3241..>3965)
                     /locus_tag="PLG78_14255"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE24333.1"
                     /translation="VHSSAKVGKNTSLGHFVTVGANSVIGNDCIIEDGVKIGEDVVIG
                     DNARIGMNCTFSYGTRIGKNFTVFGNSTFGGDGFGFYFANGIHNKIPQVGNVLIGDNV
                     EIGANCTVDRGALQDTIVKDGCKFDNMVHIAHNCNVGKNVIIAGQSGLAGSTTLGDNV
                     IVGGATAIADHLIVPDGTIVAGGTLIRNSPKEKGVYAGTEGLSFGDFQKMRANIKHLV
                     GFNKWASRIKALEKKAGITAEE"
BASE COUNT          944 a         1085 c          935 g         1001 t
ORIGIN      
        1 atttgcctcc caggtgattc tccatttcgc gcggcaaaat ggaatactga gcgatgctcg
       61 aacctaccgc aacgatgaga ggtctggtgt cagaggacct gcgtctttcc acatcgcgaa
      121 acacggattc aaaattatac cagcgaaacg aatcccatcc ggaaggattt gatagagaaa
      181 aaagaacacg gtcaaagagc agcctgtcgc acacaacgac tgcaacagga atgatcaatg
      241 caaccatcaa cgaacgaaat ctcacggcac tccctctggt ccctggagca atacaccctt
      301 acccctggcc gaatagtcaa gcagtgcgga aagtctttct gcgaggtccg attcctgata
      361 cagaaaaatt tcgcgcattt ccgccgcttc gcgcgaattc catccacccg gaatcaaaag
      421 aacaaaatcg cctgaatgta gcctgctttt cagaggtgcc tgggtatttg gaaaacgcat
      481 gagttttttc aaacgaccgg accagtatac tgcctgaatt ccatcacaga aaaatctgca
      541 atgaccatct gaaaatattt cgatggaaat ggtctccatc tgttgcggat gtttctggga
      601 ataactctgg tgagcgagtg gccaatcgtc cattttcttc aaatattcta tgaaaggtcc
      661 ctgactcgtt tgcaccgcgg atttttctcc ggccatcatt gcaaaacaca tacggtctgt
      721 tctgtgtaga atgtaacttc tgcgaaacgt ggttccacga agtccgccca cgacaccata
      781 gcgcatggtg ccccacgggg tgagcacagg gcgagaaggc acctgagctt cgcgcgcggg
      841 cggggccggt tttttccgat ccttttcgtc cgcgatgcgc aaaggatctt tctggtccgt
      901 gattggatcc gagggagctg tctgtgtgac ttcggtggct ggcgccgaga taccgggctc
      961 tgcagactcg gaaggcattt caatcgattc gatcttgccc gcttctttcg caggcaagag
     1021 agcagaagga tgaacacgtg aagaaccggc gatccatctc tcaaggctcg ttctttccgc
     1081 cggccagtac aacgttatgc ggccagcatc gtctggatca ttttctacgg cctccagata
     1141 gagcagttct gtttttgaaa agtatcctgt ttcgctgaga gtcttgattg gatcgcgagg
     1201 agcattcgtt acgtccgcac tgcgaagagc gcttccatca tcaaatagaa acgaagatat
     1261 atcgctggac caatctctag attcgaaatc gaaccgcacg cgacagccgt tttcctgaag
     1321 cgcactcttc actcgcatgg acagaggaag agatcgcacg agcgccttcc tgatcagatt
     1381 catttcactt tcttacgccg aaatgctgct ccacggttgt gaatgcagct tgaatgcgcc
     1441 ggccaagcgc tactaaaatc ttgaacgccc atctgggatg agttttgaag atcttctcaa
     1501 agttttcagg agtcaggcag agcaacttcg tgtccactgc ggcgatcaaa gtggctgatc
     1561 gcggagcgtt ttcgagcatt gccatttccc cgaagatttc tcctggcccg agtgcattga
     1621 gcacgcgata agagcctttt tcgattcttt ctgttacaac cactttgcct gaaatgatca
     1681 ggtacacctc ggagcctttg tccccttctt tgaggatgat ctccttgggt ttgtaggttc
     1741 ttccatactt gaagaataaa tccgctgtga tggcacgctc gccttcaaag ccgagcttca
     1801 tcaaatcata gtcatgagct gtttcttgca tatcagggca tctgcttcag tttgaggttg
     1861 atctgcctga gcatcttact cagaattcga atgcatgtaa gtgcgaaagt gagattggca
     1921 gccatggccc gctcgaactc gacgcgcgaa aatccaatga cctgcacctg agtggcggca
     1981 cgcgcgtccg tcattcgcgc agtgttcacg tatacctcta gcattccaaa aacatcccct
     2041 ttgccaaagc gaaacacgtc tttctgaagg ggagtgtagg cttttacaag ctccacttct
     2101 ccttctacaa tcaggtacgc gcaagggtct cctaccaggt atgcatcctg atagatcttc
     2161 tggcccgctt cgaaagtcat gggcctgcca atgcctttga tggtttccag ggagggtcga
     2221 tcgtaggtct ggtggtccat acgtgctaca gcttcctttt atccttgctt tgctttcccg
     2281 tccagcaatt cacgtctgga gatgcccgtt acctttgacg agtgcttcca gacattcccc
     2341 gccataaacc cgccaaggga tttcgcaggc ttctggaagt ccgcccttga atcactccgg
     2401 cgagtgcctg tggatccgcg ccagcgcatg gtcatcaagc gtacgctcgt gcgcgagcag
     2461 atcacagagg tccgcttcaa gagtatcggc cgctacgagc tcagaggcca gctccatgtc
     2521 cccagaaaaa ggggccgtgt tccaggggtc atcagcttcc acgattacca ggacaatcgc
     2581 attctagata cagataaggc aatcggcgac gcgggcctcg ctcatttgcg aatggattta
     2641 cgcggccacg aagcaatcgt acctggaatg ccatttactc cagtcaaaga cgcaaagctc
     2701 gatccgccct cttccagtta tccgtatctg gctgtgctcg atgctcttcg cgcagtggac
     2761 taccttcgca tccatgagtc catcgatcca aacaagatcg cagtcatcgg gcgcggtttc
     2821 ggtggatctc tcgccgcatt cgccgcagca ctctaccctg acaaaattca cgccatgagt
     2881 ctcgaaagac cttctttcct ttatctaact cgctggctgg gagaatccac gtctgtgctt
     2941 gcgcaggaag tcagagaaat acttggacca tcacgcggaa aaacaaaggc acgcaaggca
     3001 cttgattttc tcgattgcct gtatttcgca cccgcactca agcaacctgc tttctttaca
     3061 gtgggtctgg cagatgataa gagttcgcct gcgtgtgcgt ttgcactatt caatcatctt
     3121 caatcagata agacaatgga tctgtatccg gatgaagaga aagacccgct gggaacgggt
     3181 cagagaaaaa aatcggcaga attcctttcc agtgtattcg aaagcaatgc cggctaacgg
     3241 ttattcttca gcggtgatcc cggccttctt ctccagcgcc ttgattctag aagcccattt
     3301 gttgaatccc accaggtgct tgatgttcgc gcgcatcttc tggaaatctc caaaagaaag
     3361 accttctgtc ccggcgtaga ctcccttttc tttaggcgag ttgcgaatca gagttccacc
     3421 ggcaacaatc gttccatctg gcacgatcaa atgatccgcg atggctgttg ccccacccac
     3481 gatcacattg tctccgagag tagtgctacc cgcaagacca gattgtcctg caatgatcac
     3541 atttttgcca acgttgcagt tatgcgcaat gtgaaccatg ttgtcgaact tgcatccgtc
     3601 ttttacgatt gtatcctgca acgctccgcg gtccactgtg caattcgcgc cgatctccac
     3661 attgtcacct attagtacat ttcccacctg agggatctta ttgtggattc cgtttgcgaa
     3721 atagaaacca aatccatccc cgccgaatgt agagttccca aacacagtga agttcttccc
     3781 gatgcgagta ccgtaagaaa aagtgcagtt cattccgatt ctcgcgttgt ctccgatcac
     3841 aacgtcctct cctatcttca caccgtcttc gatgatgcag tcatttccaa tgacagagtt
     3901 cgcgcccact gttacaaaat gaccgagcga agtgttcttc cctacttttg cagaggaatg
     3961 aacca
//
LOCUS       DAOQJL010000366         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_13951_length_3965_cov_4.325575, whole genome shotgun sequence.
ACCESSION   DAOQJL010000366 DAOQJL010000000
VERSION     DAOQJL010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13951_length_3965_cov_4.325575"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="PLG78_14260"
     CDS             complement(<1..2237)
                     /locus_tag="PLG78_14260"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNE24334.1"
                     /translation="MNQKHQKLYELLERTQRFDKESLFRAFAHHVEYRIGKYKDNINM
                     RDVYQSVAYTIRDTLIDRWNETEEIFRKEKAKRIYYLSMEFLLGRLLETSMVNLGARE
                     MFREMLKEIDYDPEELPPQEHDAGLGNGGLGRLAACFLDSMATMDLPTFGSGLRYEYG
                     IFHQEIENGYQKEAPDNWLQYGNPWEIQRQDLTYLIKFYGHTEEYVTPTGKSLMKWVH
                     GESLLAQAYDMLIPGFQTRTIANLRLWRTRASSEFHFDYFNHGDYMRAVEDKQRSENV
                     SKVLYPNENVIQGRELRLKQEYVLVSATLQDAIKTFLEEETDFNRIPERAFFQMNDTH
                     PALAVPELMRLLMDEHAISWEQAWAITQKCTAYTNHTVMPEALEKWDLEMFGRLLPRH
                     LEIIYEINHRFLEDLKKRGIPDDVLGKVSIIEDHPMKRVRMANLAIVGSSAVNGVAAL
                     HTDLLKKDLFKEFYSFWPQKFQNKTNGITHRRWLISANQELSDLITAKIGKDWMLDLS
                     KMKALEEFAGNPEFQNDWNRVRKNNKIRLAGIIKKECGITVNPDSMFDTHIKRMHEYK
                     RQLLNLIRVIGDYQKLKANPRMEYTPRVVLFAGKAAPGYQRAKLIIKLIHSVAGVINK
                     DREIGDKLKVAFLPNFCVSLAERMYPASDLSEQISTAGTEASGTGNMKFMLNGALTVG
                     TLDGANVEMKEEAGDENIYIFGHTVEEIEKLSRSYNPVRIYEENSEIHGILNALKGDF
                     FNPEA"
     gene            complement(2396..3535)
                     /locus_tag="PLG78_14265"
     CDS             complement(2396..3535)
                     /locus_tag="PLG78_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24335.1"
                     /translation="MIVLFTEGKETTVAKADIALFREDEYGTKSYRDMLAAREEARAK
                     ELKEIESRLDEVRAFSPTGLWLGAGYGQGYLATPQEAALVGDRATLNLVPRGGQAAAY
                     FPLHGHSAKGGDAHIRFDADRFYVEAIGFSYEARGSISAIGLPPVTAAFNPGSITFNQ
                     WWTIGVFARHQNASGSVGIEPYRFSKRLRAFVFAGKSGFNTMTIADGPLFSSATILPS
                     LYFTALVQRFKTRLSGSGPRGGLELRFQAFTRLEFRASAAAASYAGITKDRGTLVIGP
                     STIPGVSGGSLIMQHGSLWVRKYDYSLGGYFAVNSRLRVFVIGSDEESRIIFRNRIPQ
                     NYLRDLNLSNYYSQDGFGEFLLRYYGRPTYTGRFRKITAGAEFRI"
     gene            complement(3706..>3965)
                     /locus_tag="PLG78_14270"
     CDS             complement(3706..>3965)
                     /locus_tag="PLG78_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNE24336.1"
                     /translation="WMGNSMQKEAIRGIYELEEAVHATGDPALLDIFRKLQTSDHFYY
                     MSTKYWSDGDVHKYFSPYGTPHDAYIYYMNALHDFRERLGM"
BASE COUNT          852 a          982 c         1018 g         1113 t
ORIGIN      
        1 tgtgcttctg gattgaagaa atcgcctttc agtgcattga gaattccgtg aatctcgctg
       61 ttctcttcgt agatgcgaac cggattgtaa gagcgcgaaa gtttctctat ctcttccaca
      121 gtgtggccaa agatatagat gttttcatct cccgcttctt ctttcatctc tacgtttgct
      181 ccgtcgagcg ttccaaccgt tagagcgccg ttgagcatga acttcatgtt tcccgttccc
      241 gacgcttccg ttcccgcagt ggaaatctgt tctgacagat cgctggcagg atacattctt
      301 tctgcgaggc tgacacagaa gtttggtaga aacgcaacct tgagtttgtc tccaatctct
      361 ctgtccttgt tgataacgcc tgccacagag tggatgagct tgatgatcaa cttggcgcgc
      421 tggtagcccg gcgctgcttt tccagcgaag agaaccacgc gtggagtgta ttccatccgt
      481 gggttggctt tgagtttctg gtagtctccg atcacgcgga tcaagttcaa gagttgcctt
      541 ttgtattcgt gcatcctctt gatgtgagtg tcaaacatgc tgtccgggtt cacagtgatt
      601 ccacactctt tcttgatgat acctgccaga cgaatcttgt tgttctttcg cacgcgattc
      661 cagtcgtttt gaaattccgg gtttcctgca aattcttcca gagccttcat cttggaaagg
      721 tcgagcatcc agtctttgcc aatcttcgct gtgatcaaat cggagagttc ctgatttgca
      781 ctgatgagcc agcggcgatg ggtgatcccg tttgtcttgt tctggaattt ctgaggccag
      841 aatgaataga actctttgaa gagatctttt ttcaggagat ccgtatgaag tgctgccact
      901 ccgttgaccg cgctcgatcc cacaatcgcc aaattggcca tgcgcactcg tttcattgga
      961 tgatcttcga tgatggaaac tttgcccagc acatcatccg ggattccgcg cttcttaaga
     1021 tcttccagga atctgtgatt gatttcgtaa atgatttcca ggtgtcttgg cagtagtctg
     1081 ccaaacattt ccagatccca tttttccaga gcctctggca tcacagtgtg gttggtgtac
     1141 gcggtacact tttgagtgat cgcccatgcc tgttcccagc tgatggcatg ttcgtccatg
     1201 aggaggcgca tcaattctgg aacggcgagt gcgggatggg tgtcattcat ctggaagaac
     1261 gcgcgttctg gaattcggtt gaaatctgtt tcttcttcga ggaaggtttt gatcgcatcc
     1321 tggagagtcg cgcttactag aacgtactct tgcttcaggc gaagttctct tccctggatt
     1381 acgttttcat ttggatagag aacttttgaa acgttttcgg atctttgctt gtcttccact
     1441 gcgcgcatgt agtcgccatg gttgaagtaa tcaaaatgga attcagaaga cgcacgcgtg
     1501 cgccacagtc tcagattcgc aatcgttctt gtctgaaatc caggaatcag catgtcgtat
     1561 gcctgggcga gaaggctttc tccgtgcacc cacttcatca ggctcttgcc tgttggagtg
     1621 acatactcct ctgtgtggcc gtagaacttg ataaggtagg ttagatcctg tctctgaatt
     1681 tcccaggggt ttccgtattg cagccagtta tccggagctt ctttctgata accgttttcg
     1741 atttcctggt gaaagattcc gtattcgtat cgcagtccgc ttccgaatgt tggaagatcc
     1801 atcgtggcca tactgtccag gaaacaggct gcgagtcttc cgaggccgcc gttccccagt
     1861 cccgcatcat gttcttgcgg tggcagttct tccggatcat agtcaatttc ctttagcatt
     1921 tcgcgaaaca tttcgcgtgc gcccaggttg accatggatg tctcgagaag tcgtccgagt
     1981 agaaattcca tggagaggta atagattctt tttgcttttt ctttgcggaa gatttcttct
     2041 gtttcgttcc agcggtcgat cagagtatcg cggattgtat aggcgactga ctggtaaacg
     2101 tcgcgcatgt tgatgttgtc tttgtatttt ccaatgcggt attctacgtg gtgcgcgaag
     2161 gcgcggaaaa gggattcctt gtcgaatcgc tgcgtgcgct caagaagttc gtatagtttc
     2221 tgatgtttct gattcattac tcccccgacg gtttgccggg aatctggatc ggttcaatag
     2281 gtcgtcaaag atttttagca tcctttctgg tttttttcca cctatcgtgc gcgtcatttc
     2341 gttcaaaaag gccagccgca attgcaaggg agttccggga ggatttactc cgggcctaaa
     2401 ttctgaactc cgcccccgct gtgatcttgc gaaaacgtcc tgtgtaagtg gggcggccat
     2461 aataacgcag gaggaattca ccaaatccat cctgggagta ataattgctc aggttgagat
     2521 ctcgcaggta gttttgcggg atgcgattgc gaaagatgat ccggctttct tcgtcggatc
     2581 caatgacaaa gacgcgtagc ctggagttca ccgcaaagta acctcccaga gaatagtcgt
     2641 actttctgac ccacaaagac ccatgctgca tgatgagaga gccgccgctt acaccaggga
     2701 ttgtcgacgg gccaatcacg agagttccgc gatccttcgt tatgccagcg taagatgccg
     2761 ccgcagcact cgcgcggaat tcgagtcgag tgaacgcctg aaagcgcagc tccagaccac
     2821 cacggggacc tgagccggat agtcttgtct tgaatctctg gacaagtgcc gtgaaataga
     2881 gagatggaag gatcgttgcc gaagagaaca gtggaccgtc tgcgatggtc atggtattga
     2941 aaccactctt gcccgcaaat acgaaggcgc gaagtctttt agagaatcga tacggctcga
     3001 tcccgacgga gccagatgca ttctggtgtc tggcgaacac tccgatcgtc caccattgat
     3061 tgaatgtaat gcttccaggg ttgaatgccg cggttacggg aggaagtcca atcgctgaga
     3121 tgctgcctct tgcctcgtaa gaaaaaccga ttgcctccac ataaaagcga tccgcatcga
     3181 accgaatgtg agcgtctcct cctttcgcag aatgaccatg gagtggaaag taagctgccg
     3241 cctggccgcc ccgcggaacc agattgagag tcgctctgtc cccgacgagt gcggcttcct
     3301 ggggagtggc caggtatccc tgtccgtagc ctgcaccgag ccatagcccc gttggactga
     3361 atgcgcgcac ttcgtccagg cgtgattcta tttctttgag ttctttggcg cgtgcttctt
     3421 ctctggcggc gagcatatcc ctgtaggatt ttgtgccgta ttcatcttct ctgaaaagag
     3481 caatgtctgc tttggcaact gtggtttcct tgccctccgt gaagagcaca atcacagaag
     3541 gatcctgaga cgtgactttt ccagtcagaa gggtgccgtc tttgaggcgg attgtgtctg
     3601 ccgcaagcga actggaaagc agtagaatgg cgatgagaaa gcgcagatca acctccggaa
     3661 gaattagacg aactcaatta cttgtcggct cggggtatgc ccggactaca tgcccagtct
     3721 ttctctaaaa tcgtgaagag cattcatata gtaaatatat gcatcgtggg gagtgccata
     3781 ggggctgaag tacttgtgaa catccccatc gctccagtat tttgtgctca tataatagaa
     3841 atggtccgag gtctgcaact tccggaatat gtcgagcagg gccgggtctc ccgttgcatg
     3901 gaccgcttcc tccaactcgt agatgccacg aatggcttcc ttttgcatgc tgtttcccat
     3961 ccacg
//
LOCUS       DAOQJL010000367         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14018_length_3954_cov_4.462939, whole genome shotgun sequence.
ACCESSION   DAOQJL010000367 DAOQJL010000000
VERSION     DAOQJL010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14018_length_3954_cov_4.462939"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLG78_14275"
     CDS             complement(<1..850)
                     /locus_tag="PLG78_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24337.1"
                     /translation="MLRTVILEIFIAARYLRGQGRMVLFSLGTRLSFMFMSFMVYTMV
                     LVLSVFNGFQSEVHKSLWNSGYHVNISRTGNPMFGFEAITQAVLKSKIVSPHVKSAFG
                     SIQVNGLLEVQGRFEGKGIRGIPTEKSGLQTGHLKDFPEIVHYNKQYLEQFDQGNYII
                     IGREMARFYGWQVGDTVHLVLPQGGSLRKGMNIRREEFIIAGFFRTGFYEFDMNLIYM
                     ALPTAQRVLDMPLQVTEVIVQLNDLSSLDFVRSTVRDELPENPYLYTIRTLKDERGNF
                     LAALQLE"
     gene            964..1941
                     /locus_tag="PLG78_14280"
     CDS             964..1941
                     /locus_tag="PLG78_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24338.1"
                     /translation="MTRLLPITSIFFFVYYLSFITGRPGLEQPEKYEDAKNTSSFKVA
                     FKPGLIERALIQKKAIGLEDDLLESDAQPERLFRSLERSYAGDLFSSAAITLWCTGFL
                     LCFLALRKRVWFGRSMAWLLILPSTVSILIVLFTLRRAGLTGVIVSYDSLAISYVETA
                     LVLIGSASLMRSGISNRDADRPFLNHLEGKDSDWADSFKRVPRAFLEIIAISAASAAL
                     ANFVVLPAYYLQISFPRFFATLLGLALAGLAAFYIRAYLKVARMQNPDVSASTAAAFL
                     GFRILANSLFITAMVSLVCLVIAMVVGIAMFNLNGLETIGVLKKASRLQ"
     gene            complement(2142..3311)
                     /locus_tag="PLG78_14285"
     CDS             complement(2142..3311)
                     /locus_tag="PLG78_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24339.1"
                     /translation="MGDRSDSTIALPARMVLTREGLSLFAKNPESIKKVKSRDGMQRE
                     GLEADAFNAPTVQKLIMNSYLEEIYVRLPDPLRRRAQIISTNNLVVYAVLYKKLSPSL
                     ARMLFESTVVREFNRKNPKNSIVDLKHIPSEKVDGLRQLKPGLFEQMEEEIHADVVDR
                     ISSNPALSEEDKSVRVRSLPKFIKWIDKRIWYLYFIIYQTAMKEQMRKAFGGMIAIYL
                     DHTKIATHLSNMLMEFVQNAEKSSLERIMVKNNLARREDVDRMLRDLDTRVKIVEIAS
                     KTDQMVDIAWNMNPERNSFGKQYRIQIIVSNYGLIDEVTRSNLARKMKTNVEGINIAS
                     FYQDSGDETKLGAGLGLLYNSYLEDICRAEGIQYKCNIFPEPEKEKTTVRVDLTM"
     gene            3417..>3954
                     /locus_tag="PLG78_14290"
     CDS             3417..>3954
                     /locus_tag="PLG78_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNE24340.1"
                     /translation="MRITVVGTGYVGLVAGTCFSEYGNKVHCVDIDEAKISKLKQGIL
                     PIYEPGLSEMVVSNYAKSRLHFTTDLKSAVEDSDIIFIAVGTPDSGDGKPDLSGVLTV
                     ASKVGEYLNDYKVIVTKSTVPVGTADQVRAAATRTAKHPFDVVSNPEFLREGRAIDDF
                     MEPERIVVGCDSERSAAMM"
BASE COUNT          948 a         1026 c          935 g         1045 t
ORIGIN      
        1 tttccagctg caacgcagcc aggaaatttc ctctttcgtc tttgagagtg cgaatcgtat
       61 atagatacgg attttcggga agctcatcgc gcactgtgct acgtacgaaa tcaagagaag
      121 acagatcgtt taactgaaca atgacttctg ttacctgtaa aggcatgtcc agcactctct
      181 gtgccgtagg cagcgccatg tagatcaggt tcatatcgaa ttcgtaaaag cccgtgcgaa
      241 agaagccggc gatgatgaac tcttccctgc gaatgttcat tcctttgcga agagatccgc
      301 cctgcggcaa aaccagatgc acagtatccc ctacctgcca tccgtagaag cgagccatct
      361 cgcggccgat gatgatgtag tttccctgat cgaattgctc gagatactgc ttattgtagt
      421 gaacgatttc aggaaaatcc ttcaaatgac ctgtttgaag ccctgatttc tctgttggaa
      481 ttccgcggat tcccttgcct tcaaaacgtc cctgcacttc cagaagtccg ttcacctgga
      541 tgcttccgaa tgcagatttc acgtgaggag aaacgatttt gctttttagg actgcctgag
      601 tgattgcttc aaatccaaac attggatttc cggtgcgcga aatatttaca tgataaccgc
      661 tattccagag ggacttatgc acttcagact gaaagccatt gaatacggaa agcacaagca
      721 ccatggtgta gaccatgaat gacatgaaca tgaaggagag cctggttccg aggctaaaca
      781 ggaccatgcg gccctggccg cgcaaatagc gcgccgctat gaatatttct agaatgactg
      841 ttcgaagcac ttcgcgtgca gtttccgaaa gctccagtct gcggtcaaca tggaactcca
      901 ggaggctcgg gcgccggcat tgaaaagaat ttacctgcag actgacgatc gaaggattgc
      961 agaatgaccc ggcttttgcc tatcaccagc atctttttct ttgtgtacta cttatcattc
     1021 atcacaggca gacccggcct tgagcaaccg gagaaatacg aagacgcgaa aaacacttct
     1081 tcattcaaag tggccttcaa gccgggttta attgaaagag ctttgattca gaaaaaagca
     1141 atcggtctgg aagacgatct tttagaatca gatgcgcaac cagaaagatt gtttagatcc
     1201 ctcgaaagat cctacgcagg agatcttttt tcaagtgcag ccatcacatt gtggtgcacc
     1261 ggattcttac tctgcttcct cgcactgcga aagagagtct ggttcggtcg ctctatggcc
     1321 tggcttttga ttcttccttc cacagtttct attttgattg tcctgttcac tctcagacgc
     1381 gctggtctca cgggagtgat tgtatcttac gattctcttg ccatctcata tgtagaaaca
     1441 gccctggtgt tgatcggatc tgcttctttg atgcgctccg gaatctcaaa cagagacgcg
     1501 gatagacctt tcctgaatca tctagaaggc aaggattcag actgggcgga ttctttcaaa
     1561 cgtgttcccc gagcatttct ggaaatcatc gccatcagtg ccgcatccgc cgcacttgca
     1621 aacttcgtag ttcttcccgc ctactatctg cagattagtt ttcctcgttt ctttgccact
     1681 cttctcgggc tcgctctggc gggccttgct gccttttaca tcagagcgta tctcaaagtg
     1741 gcccgcatgc agaatccgga tgtgagcgca agcacagctg ctgcgtttct tggctttcgc
     1801 atccttgcaa actcgctgtt catcactgcc atggtttctc tcgtctgcct tgtcattgcc
     1861 atggtcgtgg gaatcgccat gttcaatctg aatggtctgg aaaccattgg cgttttgaaa
     1921 aaagcatcca gactgcaata gtcgcctcgt gcggcccggc ggatgaacgc aatatcccgc
     1981 ctgtgcggcc catcctcgcg tgcgccccag cggcgctcgg acgctccgtt ctccgctgtg
     2041 gaccgcacat gcgggatatc actacctcgc gggccttcga gactcgcaat ttcaaaaaac
     2101 gacgcgcatc tcatcaacga ttcgcgcagc gaatcgatcc attacatagt cagatcaact
     2161 ctgactgtgg tcttttcttt ctccggctcg gggaagatgt tacacttgta ctgaatgcct
     2221 tccgctctgc atatgtcttc cagataggag ttgtacaaaa gaccaaggcc cgcacccagc
     2281 tttgtttcgt ctccgctgtc ctggtagaag ctggcaatat tgattccttc cacgtttgtc
     2341 ttcatcttcc tggcaagatt ggaccgggtg acctcatcaa tgagcccgta gttagacaca
     2401 atgatctgaa tgcgatactg cttcccgaaa gagtttcttt ctggattcat attccaggcg
     2461 atgtctacca tctggtccgt ctttgacgca atttcaacaa tcttcacgcg cgtatctaga
     2521 tcgcgaagca ttctgtccac gtcttctcga cgcgccagat tgttcttcac cataattctt
     2581 tccaggctgg atttctccgc gttctgaacg aactccatga gcatattcga caggtgagtg
     2641 gcgattttcg tgtgatccag gtagatcgcg atcattccac caaacgcctt tctcatctgc
     2701 tctttcatcg cagtctgata gatgataaaa tagagatacc agattcgctt atcaatccac
     2761 ttgatgaatt ttggcagact tcgaactctc acactcttgt cttcttctga aagagcagga
     2821 ttgctactga tgcgatccac gacatccgcg tgaatttctt cttccatttg ctcaaagagg
     2881 cctggcttga gttgtctgag tccgtctact ttctctgacg ggatgtgttt taaatctaca
     2941 atgctattct ttggattctt tcgattgaat tcgcgcacca ccgtggattc aaaaagcatc
     3001 cgtgcgagag acggactgag cttcttatag agaactgcat acacgaccag gttattggta
     3061 ctgatgattt gtgcgcgacg gcgaagagga tcaggcaggc gaacgtaaat ctcctcaagg
     3121 taggagttca tgatgagctt ttgaacggtg ggggcattga aagcatccgc ctcaaggccc
     3181 tctctctgca ttccgtcgcg gcttttgact ttcttgatgg actcggggtt cttcgcaaac
     3241 agagacagac cttctctggt taaaaccatg cgcgcaggaa gcgcgatcgt cgaatccgac
     3301 ctgtccccca tccccacagc atgattcagg ccatgcgaat ggggtcaatt gaattgcaca
     3361 cacgtgtaag cctaaaaaag aatcgcctcg gagacccgcg tcgacagctt cggaccatgc
     3421 gcattaccgt ggttggaacg ggctacgtcg gactcgtggc aggaacttgt ttctctgaat
     3481 atggaaacaa agtgcactgt gtggacatag acgaagccaa aatcagcaag ctcaagcagg
     3541 gaattcttcc aatttatgaa ccgggtctct ccgaaatggt cgtcagcaac tatgcgaaaa
     3601 gccgccttca tttcacgact gatctgaaaa gcgcagtcga ggattcagat ataatattca
     3661 tcgccgtggg cacgcctgat agcggagacg ggaagccaga tctatccgga gtactcacag
     3721 ttgcctccaa agtgggagaa tacctcaacg attataaagt cattgtaacc aaaagcacgg
     3781 tccctgtggg cacagcagac caggttcgcg ccgccgccac ccgtactgca aagcaccctt
     3841 ttgacgtggt gagcaatccg gaattcttgc gcgaaggtcg cgccattgac gacttcatgg
     3901 aaccggagag aattgtagtg ggttgtgaca gcgaacggtc cgctgcgatg atga
//
LOCUS       DAOQJL010000368         3940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14093_length_3940_cov_4.820335, whole genome shotgun sequence.
ACCESSION   DAOQJL010000368 DAOQJL010000000
VERSION     DAOQJL010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14093_length_3940_cov_4.820335"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..854
                     /locus_tag="PLG78_14295"
     CDS             129..854
                     /locus_tag="PLG78_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24341.1"
                     /translation="MKKNILRFLVLLIAGGPLHAVEKRIEYFAKAYDRNTGKFIYTEM
                     HSEIWVGGKHAGSEINYLGPDGKVLASKTIRYGSRKAAPDFKFEDKRTGYLEAAYWSG
                     QSSMEFWKRENPGDQVRQVRLNAPTSDTVVDAGFDYFIRENWERLMQQDVLVHFAVPA
                     RHSFFDFRIQRANRPASNPALVLFDVRIANPLLNLLVDKITVGYDRNSRRLMTYEGLS
                     NISDENGKSRAARIVFDYSKAGL"
     gene            854..1936
                     /locus_tag="PLG78_14300"
     CDS             854..1936
                     /locus_tag="PLG78_14300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE24342.1"
                     /translation="MDSEQTIQSEAQGPGALDRLLAHGLVPDFLIRSGIRRLNRVRLK
                     EEFAANHEVSQKRQQELIARIRKSPLAVSTREANEQHYEVPPEFFELVLGKHLKYSSA
                     YWGEGDKTLEEAEERMLKLTCQRAQLEGAKSILELGCGWGSLTLYMAEHFPSAQITAV
                     SNSQPQRLFIEARARQRGLKNITIITSDMNSFEPSDTFDRIVSVEMFEHMRNYEILFD
                     RLSKWLNLGGKLFVHIFTHKHISYLFEPRDSSDWMSRYFFTGGIMPSNHLLLYFAQGF
                     TISDHWIVNGQHYQKTSEAWLKNMDNNLHRIRRLFDETYGAESRKMIEYWRIFFMAVA
                     ELFGTKKGEEWMVNHYLFEKTGNRGS"
     gene            complement(1944..3086)
                     /locus_tag="PLG78_14305"
     CDS             complement(1944..3086)
                     /locus_tag="PLG78_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE24343.1"
                     /translation="MRFEFATSTRIVFGPGVFREIASDIRLLGKTAVIVHGKSRERAQ
                     ALAGLLEMPVVECEIAGEPRIDQIRAVMRDLHNVREPVVIGLGGGGVLDGARAVAGLL
                     TNPGDILDYLEIIGKGKTMAHRAAPLVAIPTTSGTGSEVTRNAVLTSPEHSLKVSLRS
                     PLLLPLIACVDPELTLTVPPALTANTGMDALSHILESFVSHAANPMTDAICREGLMRA
                     RSLLPAFKNGNDLAARSDMSLASLMGGMALANSRLGAVHGFAAPLGGTFDAPHGAVCA
                     ALLAPVMEVNIRALRGRDPGSPSLERYAVVAQILTNDQSVTAEDGVKWVSDLAHHLGI
                     SGISSFGVTDSHFSELCVKAAAASSMKGNPIVLQTEELQEILHRAR"
     gene            complement(3088..3396)
                     /locus_tag="PLG78_14310"
     CDS             complement(3088..3396)
                     /locus_tag="PLG78_14310"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE24344.1"
                     /translation="MFIVQVSVHVKENAIESFRIASLENARMSVQEPGIARFDVLQNR
                     EDPSRFLLIEVYRKESDAADHKQTEHYKKWRDTVESMMAEPRQSTKYSNSFPDDSGWG
                     "
     gene            complement(3406..>3940)
                     /locus_tag="PLG78_14315"
     CDS             complement(3406..>3940)
                     /locus_tag="PLG78_14315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE24345.1"
                     /translation="LYTFWIRSIVMGYISAWKISTKRTRDRGHAFISIHNTMLVDQTL
                     QAVFLVGIALFLGTLPLLAFLLSALIGILLLETVNYIEHYGLSRQRTGDRYERVRPQH
                     SWNSNHIIGRLFLFNLPRHSDHHAWASRKYQILRHFDDSPQLPAGYPAMLMLAVLPPL
                     WIPIMNARVDSLDHVVS"
BASE COUNT         1064 a         1003 c          977 g          896 t
ORIGIN      
        1 tcttccgcaa atggaattac tacctggtat tctgtgaagt tggttttgaa gctagataca
       61 tcaacgttca gcagtggctc tttggccggc ctatggctca gtccttacgt tcgatttagg
      121 agatggatat gaaaaagaac attctaagat tcctggtttt actcatcgca ggaggacctt
      181 tgcacgctgt ggaaaaaaga atcgaatact ttgcaaaagc atatgatcga aataccggca
      241 agttcatcta cacggaaatg cactccgaaa tctgggtagg cgggaagcat gcgggttctg
      301 agatcaatta cctgggtccc gacggaaagg tgctcgcatc taaaaccatt cgctacgggt
      361 cgcgaaaggc cgctcccgat ttcaaatttg aagacaagag aaccgggtat ctggaagccg
      421 cttactggtc tggacaatcg tctatggaat tctggaaacg agaaaatcca ggcgatcagg
      481 tcagacaggt tcgcctcaac gcaccgacca gtgacacggt tgtggacgcc ggttttgatt
      541 atttcattcg cgaaaactgg gaacgcctga tgcaacagga cgtgcttgtt catttcgcag
      601 tcccggctcg ccacagcttc ttcgattttc gcatccagcg cgcgaatcgt ccggcatcca
      661 atccggctct cgtgttgttt gatgtccgca tcgcaaatcc actgctcaat cttctagtgg
      721 acaagatcac agtgggctat gacagaaact cgcgcagact catgacttac gaaggtctgt
      781 ccaacatcag tgacgaaaac ggaaagagtc gcgcagcgcg catcgtgttt gattattcaa
      841 aggcaggact ataatggatt cagaacaaac aattcaatca gaggcacagg gcccgggtgc
      901 actggatcga ctactggctc atgggctggt ccctgacttc ctgattcgat ccggcattcg
      961 caggctcaat cgcgtgagac tgaaagagga attcgcagca aatcatgagg tcagccagaa
     1021 acgccagcaa gagctcatcg ctcggattag gaagagcccg cttgcagtaa gcacacgcga
     1081 agcaaacgaa caacactacg aagtcccacc ggaattcttt gaactggttc tgggaaagca
     1141 tctcaaatac agctccgcct actggggcga aggcgacaag actctggagg aagcggaaga
     1201 aaggatgctg aaactcacct gccagcgcgc tcagctggaa ggagccaaaa gcattctcga
     1261 actcggttgc ggatggggct ctctgacgtt gtacatggca gaacattttc catctgctca
     1321 gatcactgct gtttccaatt cacaaccgca acgtcttttc attgaagcac gcgctcgcca
     1381 gagaggcttg aagaacataa ccatcatcac aagtgacatg aattcttttg agccttcgga
     1441 cacatttgat cgaatcgttt cagttgaaat gtttgaacac atgcgaaact acgaaatcct
     1501 gtttgatcgg ctgtcaaagt ggttgaatct gggaggaaag ctctttgtgc acatctttac
     1561 gcacaaacac atttcctatc ttttcgaacc acgcgattct tctgattgga tgtcacgcta
     1621 cttcttcaca ggtggaatca tgccttccaa tcatctgctc ttgtatttcg cacagggatt
     1681 cacaatctcc gatcactgga ttgtaaatgg gcagcactat caaaagacgt cagaagcctg
     1741 gcttaagaac atggacaaca acctgcatcg aattagaaga ctttttgatg aaacctacgg
     1801 cgccgaaagc agaaagatga tcgaatactg gagaatcttc ttcatggcag tggctgaact
     1861 cttcggaaca aaaaagggag aggagtggat ggtgaatcac tatctatttg aaaaaactgg
     1921 caaccgcgga agctgaattg gaatcaccgc gcgcgatgca gaatctcctg cagttcctct
     1981 gtttgaagaa caatgggatt gcccttcatg ctgctcgccg cggccgcttt cacgcataac
     2041 tcagagaagt gtgagtccgt gacgccaaag gaagagatgc ctgagattcc aagatggtgc
     2101 gcaagatcag agacccattt cactccatcc tccgctgtga cactctggtc gttggtcagg
     2161 atctgagcaa cgaccgcata acgttccagg gaaggcgaac caggatcacg cccgcgaaga
     2221 gctcgaatgt tcacttccat cacaggagca agtagagcgg cgcacactgc accatgagga
     2281 gcatcaaacg tacctcccag aggcgcggca aatccgtgaa ctgcaccaag cctggaattc
     2341 gcaagagcca taccacccat caaagaagca agcgacatgt cgcttctcgc ggccagatcg
     2401 ttgccgttct taaatgcggg caatagcgag cgagcccgca tcaagccttc tctgcaaatc
     2461 gcatctgtca tgggattggc tgcatgagaa acaaacgatt ccagaatatg cgagagtgcg
     2521 tccattccag tattcgcagt tagcgccggt ggcacggtca acgtaagctc cgggtcaacg
     2581 catgcgatca agggaaggag gagaggactg cgaagactga ctttcaacga atgctccgga
     2641 gaagttaaga cagcattgcg agtgacttca gaacctgttc ctgaagtggt tggaatcgcg
     2701 acgagtggag ctgcacgatg cgccatcgtc ttccctttcc cgatgatctc caggtagtcc
     2761 agaatgtccc ctggatttgt caggagaccg gctacggctc tggctccatc cagcactccg
     2821 ccaccaccaa ggccaatcac cactggttcg cgcacattgt gtaagtctcg cataacagcc
     2881 cgtatttgat ctatgcgagg ttctccggca atttcgcatt cgacgaccgg catctcaagc
     2941 aagcctgcaa gcgcttgcgc tctctcgcgg ctcttgccgt gcacaattac ggccgtcttg
     3001 cccaggaggc gaatgtcaga agctatttcc cgaaaaactc cgggaccaaa gacaattcgt
     3061 gtagaagtcg cgaattcaaa acgcacttta tccccagcca gagtcatctg gaaacgagtt
     3121 ggaatatttt gtactctgcc gtggttcagc catcatggat tccacagtgt cacgccattt
     3181 cttatagtgc tcggtttgct tgtgatccgc tgcgtccgat tctttgcgat acacttctat
     3241 cagtaaaaaa cgggaaggat cttctctatt ttgaagaacg tcaaagcgag caatccctgg
     3301 ctcctgcacg ctcatcctcg cgttctccag ggaagcaatg cgaaagcttt cgattgcatt
     3361 ttctttgacg tgaacactca cctggacaat gaacatggca acctctcagg aaacaacgtg
     3421 atccagcgag tcgacacgcg cattcataat aggaatccat agaggcggaa gaacagcgag
     3481 catgagcatg gccggataac ctgcgggcag ctgcggacta tcatcaaaat gacgaaggat
     3541 ctggtacttc cggctggccc aggcatgatg atccgaatgc cgcggaagat taaacagaaa
     3601 caatctaccg atgatgtgat ttgaattcca cgaatgttga ggtcgaactc tctcgtacct
     3661 gtctcccgtt ctctggcgcg aaagtccgta gtgctctatg taattcaccg tttcgaggag
     3721 aaggattcca atcaacgcgg acagaagaaa tgcaagcaga ggtagagtcc ccagaaaaag
     3781 agcgatcccc acgagaaaga cggcctgcaa agtctgatct acgagcattg tgttatgaat
     3841 agatataaac gcatgcccgc gatccctggt tctcttcgtg gatattttcc acgcggatat
     3901 ataacccatg acaatggaac gaatccagaa agtatagagc
//
LOCUS       DAOQJL010000369         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14128_length_3933_cov_4.108045, whole genome shotgun sequence.
ACCESSION   DAOQJL010000369 DAOQJL010000000
VERSION     DAOQJL010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14128_length_3933_cov_4.108045"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="PLG78_14320"
     CDS             <1..1850
                     /locus_tag="PLG78_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE24346.1"
                     /translation="EVFSAVSYFNWRADMKRFSAYLIHKLSCEPGTKVGLLCDNRYEW
                     NLISMGITTIGCIDVPRGCDATHQDINYILNHTECPVLVVEHEKMLKTVVGMLGELPS
                     LKHIISIEGPEKFKNLDEIASKLGKTQLHFLVDTFAEGEEILAKLGEGFLKKRGEEIR
                     PHSIATIIYTSGTTGSPKGVMLEHRSFCWEVNQMQQVLPLGERDRTVIFLPPWHIAER
                     LLETTLIACGSSMANASIISLAADMGVIKPTALVSVPRVWEQLYRRVFDNVRKQPEKT
                     QNIFNFALNAAQTYTDIMDTLLDRFAETEDEEKTSTLTRKAIAAAMLPVFAILNIPAQ
                     IILKKVKNIFGGRLKFAVSGAGALPEHVAVFFRAVGIPILDGYGMTETTGVAAISALP
                     FPRRGCVGRPIPGAQIQLRDDTGKVITRPGVKGVCWHKGPHVMRGYYKADDKTAEIMR
                     DGWLNSGDIFVWTNTGEIKFAGRAKDTIVLAGGENVEPGPIEMKLLEHPFISQVVVVG
                     QDKKTLGALIVPNYDRCKEEFKNMGFEAPSDYAQWDTHKEIHKFFQDIVKQQVSGSHG
                     FKSFEKVTGFHIMRKEFEKGKEMTETMKIKRNVVFDLYKKEIDSLYRDD"
     gene            complement(1879..2268)
                     /locus_tag="PLG78_14325"
     CDS             complement(1879..2268)
                     /locus_tag="PLG78_14325"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE24347.1"
                     /translation="MLEPGLASGILEIRSPKRRSLTMHDHRSVITHSMAISGDLTVDT
                     HLIIEGAISATKIDSKNHTVVVGQYSAVHGDVYGSQILVLGEINGNLFASEKIDIRST
                     AVINGDVNAPLIKMEEKSRLNGRLNYT"
     gene            complement(2372..3139)
                     /locus_tag="PLG78_14330"
     CDS             complement(2372..3139)
                     /locus_tag="PLG78_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE24348.1"
                     /translation="MSFVNVQTNGPLSILTLKRPEAKNALNREVLRDIREAVSKIAQD
                     PTQRVMILTGDGAAFCAGADIAEMKGLSAEQAEEFARNGHDTMNAVAASDLISIAAIN
                     GFALGGGLELALACDIRIASSTAKMGLPEVGLGLIPGFGGTQRLSRLIGRARALEMVC
                     SGEIITAEQAASVGLVQKVSAPESLMADANALAEKFLKGKAPSAQKTARWLVISGMEL
                     PLSAGIEREINAFAELFNTKEPVEGLSAFLEKRSPNF"
     gene            3229..>3933
                     /locus_tag="PLG78_14335"
     CDS             3229..>3933
                     /locus_tag="PLG78_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNE24349.1"
                     /translation="MRQRVFFEDLRLELENLSSHGLTALKTGTEVEDMSFEEIRILRA
                     LSRDICPLFVKIGGAEARNDIRELAEIEVDAIIAPMIESVYALEKFIESMRDVLSPKA
                     YRRIEKGFNLETATAFNRMTELLTSEAAQELVQVTAARTDLSGSLRVSPEHEIIFELC
                     THIVARCRQYGLRTSVGGAIKSASIEKLVGQIGPDTVNTRHMVMDSAVLAGNPVCVLE
                     KHLMWEVHLYQYLASFP"
BASE COUNT         1022 a          971 c         1013 g          927 t
ORIGIN      
        1 cagaagtctt ttccgcagtc tcgtatttca actggcgtgc ggatatgaag cgcttctcgg
       61 cgtatctgat ccacaaactc agctgtgaac ccggaacaaa agtgggtcta ctgtgcgaca
      121 acagatacga atggaatctg atcagcatgg gtatcaccac gattggctgc atagacgtgc
      181 ctcgcggctg cgatgcaacg catcaggata tcaattacat tctcaatcat actgaatgcc
      241 cggtgctcgt ggtcgagcat gagaagatgc tcaagacggt agttggaatg ctgggagaac
      301 ttcccagtct caaacacatc atatccatcg aaggtccgga aaaattcaag aacctggatg
      361 agattgcttc aaaactcggg aaaacacagc ttcattttct ggtggatacg ttcgcagaag
      421 gagaagagat tctcgcaaag ctgggagaag gttttctaaa aaaacgcgga gaagaaatta
      481 gaccgcactc gattgccact atcatctaca catccggaac tacgggatcc cccaaaggtg
      541 taatgctgga gcacagaagc ttctgctggg aagtcaatca aatgcaacag gttctgcctt
      601 taggagagcg agatcgcacc gtgattttcc ttccgccatg gcacatcgca gaaagacttc
      661 tggaaaccac tctcatcgca tgcggttcca gtatggcaaa tgcgagcatc atatctctgg
      721 ctgcggatat gggagtaatc aaaccaacgg ctctggtgag tgtgccgcgc gtatgggaac
      781 aattgtatcg tcgcgtgttt gacaatgttc gcaaacaacc agaaaaaacg cagaacatct
      841 tcaacttcgc tctgaacgcg gctcaaacat acacggatat catggacact cttctggatc
      901 gcttcgcgga aacagaggat gaagaaaaaa catccacact tacgcgaaag gcaatcgccg
      961 cggccatgct tccggtcttc gccatactca atattcccgc ccagatcatc ctgaagaaag
     1021 tgaagaatat ttttggcgga cgtttgaagt tcgcagtctc cggcgccggc gcattgccgg
     1081 aacacgtggc tgtattcttt cgcgcagtcg gaattccaat tctcgatggc tatggaatga
     1141 cagaaacaac aggtgtggcc gcgatctctg ccctgccctt ccccagacga ggctgtgtag
     1201 ggagacctat ccctggagct cagatccagc ttcgcgatga tacaggaaaa gtgatcacca
     1261 ggccaggggt aaaaggagtc tgctggcaca aaggaccgca cgtgatgcgc ggctattaca
     1321 aggcagacga caaaacagcc gaaatcatgc gtgatggttg gctgaactca ggcgatatct
     1381 tcgtttggac caacactggt gaaatcaaat tcgcgggccg tgccaaagac acaattgttc
     1441 tggcgggtgg cgaaaacgta gaaccggggc caatcgaaat gaagctgctt gagcatcctt
     1501 ttatttcgca ggtcgtggtg gttggtcagg acaagaagac tctgggcgct ttgatcgtac
     1561 ctaactatga tcgatgcaaa gaagaattca agaacatggg atttgaagcc ccatcagatt
     1621 acgctcagtg ggacactcac aaagagattc ataaattctt ccaggacata gtcaagcagc
     1681 aggtttccgg ttcgcacgga tttaagagtt tcgagaaggt cacaggattc cacatcatgc
     1741 gcaaggaatt cgagaaaggg aaagagatga ctgaaacgat gaagattaag cgaaacgtag
     1801 tgtttgatct ttataaaaag gaaatcgact cactctacag agacgattga tctctgatct
     1861 ctgtagagtt ccaaacgatc aggtatagtt gagacggccg ttgaggcggg acttttcttc
     1921 catcttgatc agcggagcgt ttacatcgcc gttgatcaca gctgtgctgc gaatgtcgat
     1981 tttttcagat gcgaaaagat ttccgttgat ttcgcccaga accagaattt gagaaccgta
     2041 cacatcgcca tgaactgcgc tgtactgtcc aaccacgact gtgtggttct ttgaatcaat
     2101 cttggtggcg gaaatagcgc cttctatgat gaggtgagtg tctaccgtaa ggtctccaga
     2161 gattgccatg gaatgagtga ttacggatct atgatcatgc attgttaaac tcctgcgttt
     2221 gggtgagcga atctcaagaa tgccgctggc taacccgggc tcaagcattt tagaaaaaaa
     2281 agttgcccga ctgtccggtc aatgtgacag aatccgcctc aagggccggg ccagagacgg
     2341 ggccttctag aacagaaacc tggaagaaat gttaaaaatt tgggctgcgc ttctcaagaa
     2401 atgcagacaa accttcgacc ggttcttttg tgttgaagag ctctgcaaac gcgttgatct
     2461 cgcgttcgat gcccgcgctg agcggaagtt ccattccact gatcacaagc cagcgcgccg
     2521 tcttctgagc ggaaggcgcc ttacccttta agaatttttc tgcaagtgca tttgcatccg
     2581 ccatcagaga ctcgggtgcg gaaacttttt gcaccaggcc aacggaagca gcctgttccg
     2641 ctgtgatgat ttcgccggaa caaaccattt cgagggctct ggcccggccg atcaaacgtg
     2701 aaagcctctg cgttcctccg aatccaggaa tgagccccag acccacttcc ggaagcccca
     2761 tcttagctgt actggaggcg attcgaatgt cgcatgcaag agccagttcg aggccaccgc
     2821 ccagtgcgaa accgttgatg gcagcaatcg agatcaaatc cgaagcggcc accgcattca
     2881 tggtatcatg accattgcga gcgaattcct ccgcctgctc cgcgctcagt cccttcattt
     2941 ctgcgatatc cgcacccgcg caaaatgcgg cgccgtcccc agtgagaatc atgacacgct
     3001 gggtagggtc ctgggcaatt ttggaaacgg cctcgcgaat atcccggagc acttcgcggt
     3061 tcagcgcgtt ttttgcttcc ggccttttga gggttaggat ggagagtgga ccgtttgtct
     3121 ggacatttac aaaagacatg agcagttttc tcggccgcgc ggtttttccg ctattaattt
     3181 cttctaaatg aattctgccg ggggtcgaaa ctgttaaagg ggatcccaat gagacaacgc
     3241 gtgttcttcg aagatctgag actggaactc gaaaatctct cttcgcacgg cctgaccgcc
     3301 ctgaagacag gcacagaagt ggaagatatg tcctttgagg aaatccgaat cctccgcgcc
     3361 ctctctcgtg atatttgccc cctctttgta aagatcggtg gtgccgaggc tcgcaatgat
     3421 attcgcgaac tggcggaaat cgaggtcgat gcgatcatcg ctcccatgat cgagtccgtt
     3481 tacgcactcg agaaattcat tgaaagtatg cgtgacgttc tgagcccaaa agcctacaga
     3541 cgaattgaaa agggtttcaa cctggaaaca gcaacggcgt tcaatcgcat gactgaattg
     3601 cttacctcag aggcggcgca ggaactggtc caggttacag cagccagaac agatctttcc
     3661 ggatcgctgc gcgtttcacc tgaacatgaa atcatttttg aattgtgcac tcatattgta
     3721 gcgcgctgca ggcagtatgg tcttcgcacc tcagtgggcg gtgccatcaa gtctgcttcc
     3781 attgaaaaac tcgtgggtca gattggcccc gacactgtga acaccaggca tatggttatg
     3841 gattcggctg tgcttgctgg aaatccggtg tgcgttctag aaaagcattt gatgtgggaa
     3901 gtgcaccttt accagtacct cgccagtttt cct
//
LOCUS       DAOQJL010000370         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14148_length_3929_cov_4.097315, whole genome shotgun sequence.
ACCESSION   DAOQJL010000370 DAOQJL010000000
VERSION     DAOQJL010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14148_length_3929_cov_4.097315"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1930)
                     /locus_tag="PLG78_14340"
     CDS             complement(26..1930)
                     /locus_tag="PLG78_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE24350.1"
                     /translation="MTSREEITRAENYYRAISELTVARHREHLIKELLNYMASLLNAV
                     EEANSRLTFSEARIRAIVNTAADGILTYDESGNIDSINPAAAAMFGLNPESIQGLHVS
                     SLLEGESEGHLRTVNARQVGGGYEDVAKRGNTTFPVYIAVQDMRLADRMFHVAILRDI
                     TDQKNAQRLEAQARAKEETDKILSAIDQGLFLIYRDGARFEIGSQFSRATRDILGVDL
                     ESQKDLLSILGNRVAESTIAAAKNFLELMFKDSVREESLRSLNPLSEIPFASQTAGDT
                     RTLQFTFRRVTTDQKITHLMATVSDVTEEAALREQLKQNEQQSRTQMEMLLKILHVDS
                     DMLDEFIDETRLEIDIIDTILKKEDGDLRKRLETIFRTAHKIKGNADLLGLSFFAEQI
                     HSFEDTLSVLLRNEEISPADFLPVLFAFNKVTEIVDAIDALVERLLDFKSRFGQSNSG
                     DELILRSARALINKLTDETGKLAELHVRVFEARTLGTPKRKLIRDVITQLIKNSMMHG
                     IETPETRTVGGKRNPARIELNLRSGKDGIFLNYKDDGQGLHLSRLKEKAIKQGIATEA
                     EIATWPPAKTAELIFATGLSTSLTTGIQSGRGIGMGLIREAVHDVQGKIRILSAPAKF
                     FEVQIWIPVA"
     gene            complement(1927..2715)
                     /locus_tag="PLG78_14345"
     CDS             complement(1927..2715)
                     /locus_tag="PLG78_14345"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24351.1"
                     /translation="MSAGAAFPVVDTDHAVEIAPEVFWTGYYMEDEALHCNPYLIRNG
                     TESILIDPGSVPDFPVVGRKLFSVIKPESVTTIILNHQDPDLCASVPIFESLRENFDY
                     DVIAHRKTIYLLKHYGIKGKFHRLDDGETAYKTPGGRELKFITTPFAHFAGAVMTYDV
                     KSKVLFTGDILGGVGTDWELYHTDIALANMKAFMQGFMPCNKALRYALLKIESSGAEL
                     FAPQHGQIIHRDQFPSIKEALWDIKCGLDLIEDDAMKKAMVHAV"
     gene            complement(2712..3161)
                     /locus_tag="PLG78_14350"
     CDS             complement(2712..3161)
                     /locus_tag="PLG78_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE24352.1"
                     /translation="MTQKDLQIFVNGTVNYFKQLTDSGAQMDVPFLKKPGETAALDIT
                     GVIGISGVKKGIIYFTASSDMLRDVVKTILGTENPDLASIVDIAGEIANTIAGNAREA
                     FGADFMISIPLVVEGPPKNIRIPNEVPVFVIPIRWKDHRSFLVVGIQ"
     gene            complement(3158..3523)
                     /locus_tag="PLG78_14355"
     CDS             complement(3158..3523)
                     /locus_tag="PLG78_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24353.1"
                     /translation="MKLLIVDDSNIIRKAIQKYLAEFGLEIVGEAGDGRTALTLFRET
                     SPDVVTMDITMPEMDGLETLSEMRKISDSPKIIIVTALKDKATALDALQKGASGFLAK
                     PFTEAQLKEEIGRAIGRAS"
     gene            complement(3555..3827)
                     /locus_tag="PLG78_14360"
     CDS             complement(3555..3827)
                     /locus_tag="PLG78_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24354.1"
                     /translation="MRARAHLRGQELAVPLEGIDECFLTRWIDSRPDLANRPQCVSLK
                     RAHEALHKIVAKKDSGPATTDLLQDFEIQSRAVMTAIETMRQAIRP"
BASE COUNT          844 a          980 c          985 g         1120 t
ORIGIN      
        1 atagacgctg cgccctggaa agacatcatg cgaccggaat ccagatctgt acttcgaaaa
       61 actttgcagg cgcggataga attctgatct taccctgaac gtcatgcact gcttcgcgga
      121 tcaaacccat tccaatgccg cgtcccgatt ggattccagt tgtcagtgaa gtggaaaggc
      181 ctgtggcgaa tatgagttcc gccgttttcg cgggtggcca ggttgcgatt tcagcctctg
      241 tggcgattcc ctgcttgatt gctttctcct tcaatcgtga aagatgaaga ccctgtccgt
      301 cgtctttata gttgaggaag attccatctt tacctgaacg caggttcagt tcgattcttg
      361 ccggatttcg ttttccgccg acagtgcgcg tttccggagt ttctattccg tgcatcatgc
      421 tattcttgat gagctgagtg atgacatcgc ggatcaattt gcgtttaggc gtgccaagag
      481 tgcgggcttc gaatacacga acatgaagtt ctgcgagctt acctgtttca tccgtcagct
      541 tgttgatgag cgcccgggct gagcgaagaa tcaattcatc tccgctattg ctctgtccaa
      601 atctactttt gaaatcgagc aggcgctcca ccagggcgtc aattgcgtcc acaatttccg
      661 tcactttgtt gaatgcaaaa agcaccggaa ggaaatcagc gggagagatt tcctcatttc
      721 gcaagagcac cgacagtgtg tcttcaaagc tatgaatctg ttctgcgaag aaagaaagac
      781 ccagtaaatc cgcattgcct ttgatcttgt gcgcagtgcg aaagattgtc tccaatctct
      841 ttcgcaggtc tccatcttcc tttttcagaa tggtatcaat gatgtcgatt tcgagacgag
      901 tttcgtctat gaattcatcg agcatatcag agtctacgtg caggatcttc aaaagcattt
      961 ccatctgcgt tctggactgc tgttcgtttt gtttgagttg ttctcgcagt gctgcttcct
     1021 ctgttacgtc acttacggtt gccatcaagt gagtgatctt ctggtctgtc gtgactctgc
     1081 gaaacgtaaa ctgaagcgtg cgcgtgtctc ctgcggtctg ggaggcgaat ggaatttcag
     1141 agagaggatt gagagagcga aggctttctt cgcgcaccga atccttgaac atcagctcca
     1201 ggaaattctt tgcggccgca atcgtgcttt cagccactct gtttcccaga atggaaagca
     1261 agtccttctg gctttccagg tccacaccca gaatgtctcg cgtggctctg gagaactgtg
     1321 acccgatttc aaatcgtgct ccatcccgat aaatcaagaa cagaccctga tcaatggcag
     1381 aaaggatctt gtctgtctct tcctttgctc tcgcctgtgc ctcgaggcgt tgtgcattct
     1441 tctgatctgt gatgtctctg agaatggcca cgtggaacat gcgatcagcc aggcgcatgt
     1501 cctgaactgc gatatagacg gggaaggtgg tgtttcccct ctttgccaca tcttcgtagc
     1561 cgccgccaac ttgcctggcg tttacggtcc gcaggtgtcc ttcggattcg ccttcgagga
     1621 gagaggaaac gtgcaggccc tgtatggact caggattcaa tccaaacatg gcggcggcgg
     1681 cagggttgat gctgtctatg ttgccgcttt catcgtaggt gagaatcccg tctgctgcag
     1741 tattgacaat ggcccggatg cgagcctcac tgaaagtaag tctggagtta gcttcttcga
     1801 cggcgttgag aagagaagcc atgtagttga gcaactcttt gatgagatgt tcgcgatgtc
     1861 tggcgacggt cagttcactg attgctcgat aataattttc cgcgcgcgtg atttcttcgc
     1921 gcgatgtcat accgcatgaa ccatcgcttt tttcatggca tcgtcttcta tcaagtcgag
     1981 tccgcacttt atatcccaga gcgcttcctt tatggaggga aattggtctc tatgaatgat
     2041 ctgtccgtgc tggggcgcaa aaagttctgc tccggacgat tctatcttca agagcgcata
     2101 acggagcgcc ttgttgcatg gcatgaatcc ctgcataaac gctttcatgt ttgccagtgc
     2161 tatatccgta tgatacagct cccagtctgt gccgactccg ccgagaatat ctcctgtaaa
     2221 aagaactttt gattttacat cgtacgtcat tactgctccc gcgaaatgcg cgaaaggagt
     2281 tgtgatgaac ttgagctctc tgccaccggg agttttgtat gcagtttccc cgtcgtccag
     2341 tctgtggaat tttcctttga ttccatagtg cttcagcaaa taaatagtct tgcgatgggc
     2401 tattacatcg tagtcaaagt tctcgcgcag actttcaaag atcggcacgg acgcgcaaag
     2461 atcaggatcc tgatgattga gtatgatcgt tgtgaccgat tctggtttga tcacggagaa
     2521 tagttttcgt ccgactacag ggaagtcagg tacagagcct ggatcaatca gaatggattc
     2581 agttccgttt cgaatgagat acggattgca atggagcgct tcgtcttcca tgtagtagcc
     2641 ggtccagaat acttctggcg cgatttcaac cgcgtgatcc gtatctacaa ctggaaaggc
     2701 tgctcccgcg ctcactggat tcccaccact agaaaagatc gatggtcctt ccaccggatc
     2761 ggaatgacga acacgggcac ttcattcggg atgcgaatat tctttggcgg accttccaca
     2821 acgagcggaa tggagatcat gaagtccgct ccaaatgcct ctcttgcgtt tcccgcaatc
     2881 gtatttgcga tttcgcccgc gatatcaaca atcgatgcga gatccggatt ttcagttccg
     2941 aggattgtct tgactacatc cctgagcata tctgaggacg cagtaaaata gataatcccc
     3001 ttcttcactc ccgagatccc aatcactccc gtgatatcga gtgcagcggt ttcgcctggt
     3061 tttttcagga agggaacgtc catttgcgct cctgaatcag tcagctgctt aaagtagttt
     3121 acggttccat ttacaaagat ttgaagatcc ttttgagtca tgatgctctc ccgatcgcgc
     3181 ggccgatctc ttccttgagt tgtgcttccg tgaacggttt tgccagaaat ccagacgctc
     3241 ctttctggag tgcgtcgagt gcggttgctt tgtctttgag tgctgtaaca atgatgattt
     3301 taggagagtc gctgatcttt cgcatttcgc tgagtgtttc cagtccgtcc atctctggca
     3361 tcgtgatgtc catcgtcaca acatctggag atgtttccct gaatagagtc agagcagttc
     3421 gcccatctcc tgcctctccc acgatttcaa gcccgaactc ggccaggtat ttttgaatcg
     3481 cttttcgaat gatgtttgag tcgtctacaa tcagtagttt cacatgtttt ctcccgcggg
     3541 ttgtgtttcg aggttcatgg tcgaattgcc tggcgcatgg tttcgatggc cgtcatcact
     3601 gcgcgcgact ggatttcaaa atcttgaagg aggtctgtcg tggccgggcc tgaatctttt
     3661 tttgcgacga ttttgtggag ggcttcgtgt gcacgtttca gagaaacaca ttgaggtctg
     3721 ttcgcaagat ccggtctgga atcaatccac ctggttaaga agcactcgtc tatcccttcg
     3781 agcggaactg caagttcttg cccgcgcagg tgcgcacgtg cgcgcactct ccattgtatg
     3841 ttgtgcctga acggcccggt caaagtccac ttccgccagg tcaagtccgg acttgatcct
     3901 ggtcaagtcg tgcgtgcatt tgaggggtc
//
LOCUS       DAOQJL010000371         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14155_length_3928_cov_4.836303, whole genome shotgun sequence.
ACCESSION   DAOQJL010000371 DAOQJL010000000
VERSION     DAOQJL010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14155_length_3928_cov_4.836303"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLG78_14365"
     CDS             complement(<1..459)
                     /locus_tag="PLG78_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE24355.1"
                     /translation="MAEPALMLSLTEEQLQLRDLVREVVKNEVVPVRMELDEKNEYPR
                     RVLEKFREAGIFQALFPEEYGGLGMGMMANIILSEEIAYGCLGVGTAFLATKLGSLPI
                     EVGATEEQKKKWLPQLASGEKVAAFGLTEPGAGSDVPALSTTAVKKGDRYI"
     gene            complement(576..1913)
                     /locus_tag="PLG78_14370"
     CDS             complement(576..1913)
                     /locus_tag="PLG78_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNE24356.1"
                     /translation="MSDPLIHILIILGMILLNGFFAGSEIAIISLRKARVKMLLEQGD
                     PRAKTVQGLQSSPERFFATVQVGITVLGTLMSMYGAEQYADKLAPVLAGWLAPVPLLA
                     DYSLQISLGILVVIFSYLMLVLGDLAPKSLGHRYAEKFALAAAGPLLAFSRVFQVFIR
                     ISTWSANIVLWPFKDRTSFSETKLLPEEILQLLEEGVKTGSIAHSEHEIIENVLEFKD
                     TVAREVMIPRVDMKAVDVESSPEDAAVSLLDQFHSRLPVFSDNLDHIVGILHIKDLMK
                     ARARGDRYTLSTLARPAFFVPETMKIGDILKEMQKRKTHMAIVVDEYGGTSGLLTMED
                     ILEEIVGEIEEVTEQSAQGIESLPDGSYLVPGGCSIPDFNEYFDAMLEESDAYTSVAG
                     YLIHKAGRFPEVGETMTAESMQFELVRRVRQKLVQFRVTRVEPAPTAVDESKT"
     gene            2033..2785
                     /gene="map"
                     /locus_tag="PLG78_14375"
     CDS             2033..2785
                     /gene="map"
                     /locus_tag="PLG78_14375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE24357.1"
                     /translation="MIHIKSNTEIQKMKDAGKLAAEVLNETGRLVRPGVTTLELNDFA
                     HRLTLKRGAESAPLNYRGFPKSICTSINNVVCHGIPKKGETLKEGDIVNLDITVKLDG
                     YHGDTSRTFAVGKISDAAQKLMEDTEKSMWVGIEVVRPGARICDVGDSIDAFLTPKGY
                     GIVRDLCGHGIGRNFHEEPQVPHYKNNGIRSVMRPGMTFTVEPMVNRGSYEVFTDAHD
                     GWTVWTRDKSLSAQFEHTVLVTDDGYEVLTRE"
     gene            2782..3300
                     /locus_tag="PLG78_14380"
     CDS             2782..3300
                     /locus_tag="PLG78_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24358.1"
                     /translation="MNRLLLALLVLTSCVSGQNGSYPNFTSIELREYGIILHHPALWD
                     LNFDSKRKLPLVARGFTHDGRRASIEYRGIPQTQEDVDLYAEGWYKAMPSNFDSFEMV
                     DRKKISTEQGIVYHFEGTFREGDQRQRVVGRLRIRDGRVHAIYYLADDKDFSSFREIL
                     EDLDQSHRYFRP"
     gene            3391..3462
                     /locus_tag="PLG78_14385"
     tRNA            3391..3462
                     /locus_tag="PLG78_14385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3424..3426,aa:Arg,seq:ccg)
     gene            3465..3851
                     /locus_tag="PLG78_14390"
     CDS             3465..3851
                     /locus_tag="PLG78_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24359.1"
                     /translation="MELVLKYIGIGLGAALAGWGMFHQLIVGGAVSMIKHPDDSEARL
                     FVMSWVAQGGFMSFLGVIPATLLLIYGPSTAAVHTVLSLSALALILLSSHVFLTGFKT
                     HLRPIQIGASLELIYGIYLVILIVLS"
BASE COUNT          983 a          988 c          989 g          968 t
ORIGIN      
        1 aatgtaacga tctcctttct tcacagccgt tgtggaaagt gctggaacgt ctgaacccgc
       61 acccggttct gtaagaccga acgcagctac tttttccccg cttgcaagtt gcggaagcca
      121 cttcttcttt tgttcttctg tcgcgcccac ttcgatcggg agagatccaa gcttggttgc
      181 gagaaacgca gtgcccactc caaggcatcc gtatgcgatt tcttcagaca ggatgatgtt
      241 ggccatcata cccattccaa gtccaccgta ctcttctgga aacagtgcct ggaaaatgcc
      301 cgcttcacgg aacttctcaa gtacgcgacg cggatattca ttcttttcat ctagttccat
      361 gcgcacagga actacttcat ttttcacaac ttcacgcact agatcgcgaa gctggagttg
      421 ttcttcagtt aaggaaagca tcaatgctgg ttcagccata cgaccttttt tttaggtttg
      481 gcatttgagg ctagaagatt ttggaacgag tcgtcgcgga caatcgcgtc cggaatgctc
      541 gtaaatgaga cataatctaa agtgctttcg gagatttaag tcttactttc gtccaccgca
      601 gttggcgcgg gctccactct ggtgacgcga aattgaacca gtttctgacg cactcgtctg
      661 acgagctcaa actgcatcga ttccgctgtc atggtttctc cgacttccgg aaagcggccc
      721 gctttgtgaa ttagatagcc ggccacgctg gtgtaggcgt cggactcttc gagcattgca
      781 tcaaagtatt cgttgaaatc agggatggaa caacctccag ggaccagata gctgccgtct
      841 ggaagggatt caattccctg ggccgattgc tccgttactt cctcaatttc cccaactatc
      901 tcctccagga tgtcctccat ggtaagaagg ccggaagttc cgccgtattc atccacgacg
      961 atggccatat gagtctttct cttctgcatc tcttttagaa tgtcgcctat cttcatagtc
     1021 tcgggcacga agaacgcagg acgagcgaga gtactgagtg tgtagcgatc tcccctggca
     1081 cgtgccttca tgagatcctt gatgtgcaga atcccgacta tgtgatcgag gttatccgag
     1141 aaaacaggaa ggcggctgtg aaattgatcc agaagggaca cggccgcatc ttccggagag
     1201 gactctacgt ccactgcctt catatctacg cgtggaatca ttacttccct ggcgactgtg
     1261 tccttgaact cgagtacgtt ctctatgatt tcgtgttcgc tgtgcgcaat gctaccagtc
     1321 tttactcctt cttcgagaag ttgcaggatt tcctccggca agagcttggt ttctgagaag
     1381 ctcgttctgt ctttaaatgg ccagagtact atgttggcag accaggtaga tatgcgaatg
     1441 aagacctgga agacacgcga gaaagccagg agcgggccgg ctgccgccag cgcgaacttc
     1501 tccgcatacc tgtgccccag agatttgggc gccaggtcac cgagaaccag catcagataa
     1561 gagaatataa caacgagtat gccaaggctg atctgtagag agtaatcagc taacaggggc
     1621 acaggtgcca gccagccggc cagaactggc gccagcttat ccgcatactg ctctgcgcca
     1681 tacatggaca tcaaagtccc cagtacagtg atacccacct ggacggtggc aaagaaacgc
     1741 tcggggctgg actggaggcc ctggactgtt tttgcgcgcg gatctccctg ctccagaagc
     1801 atcttgaccc gagcctttcg cagcgatata atggctatct cgctgccggc gaaaaatccg
     1861 ttcagcagga tcattcctaa aatgattagt atgtgtatga gcggatcaga cattcaaaag
     1921 aggacaaaga atcatcgaac tttgccctcg aacgggctct gcacgatcag gtgcgcaagt
     1981 taatggatgc cgcgcggcct gccagtcttt ttttttaaca atgtagaacc aaatgataca
     2041 cataaaaagc aacactgaaa tccagaaaat gaaagatgcg ggcaaactgg ccgcagaagt
     2101 actcaatgag acaggccgcc tggttcggcc aggggtcacc acgctggaac tgaacgattt
     2161 tgctcataga ttgaccttga aacgcggggc ggaaagtgcc cccctgaact accgcggttt
     2221 cccgaagagc atttgcactt ctatcaacaa cgtggtgtgc cacggcattc caaagaaagg
     2281 agaaaccctc aaagaggggg acatcgtaaa cctggatatc acagtgaaac tggatggata
     2341 ccacggcgac acatctagaa cattcgcggt tggtaagatc agtgatgcgg cgcagaagct
     2401 catggaagac accgagaagt ctatgtgggt cggcattgaa gtagtcagac caggtgcgcg
     2461 tatctgcgat gtcggagact ccattgatgc gtttctgact ccgaaaggat atggaatcgt
     2521 tcgtgatctg tgcggtcacg gaattggcag gaactttcac gaagagcccc aggtcccaca
     2581 ttacaagaac aatgggatcc gatccgttat gcggcctgga atgaccttta cagtagaacc
     2641 aatggtaaat cgcgggagtt atgaagtatt tacagatgct cacgacggat ggaccgtctg
     2701 gaccagagat aaatctttat cggcacagtt cgagcacacg gtcctcgtaa ccgatgatgg
     2761 atatgaggta ctcactcgcg aatgaatcgt ttgttgcttg cactgctggt gctgacatcc
     2821 tgtgtcagcg gccagaacgg ctcgtatcca aatttcacgt cgatcgagtt gcgtgaatac
     2881 ggaatcatcc tgcaccaccc tgccctctgg gatttgaatt ttgacagcaa gcgaaagcta
     2941 cctctggtcg cgaggggatt cactcacgat ggaagacggg cgagcattga gtatcgcggg
     3001 attccgcaaa cgcaagaaga tgtagattta tatgcagaag gttggtacaa agccatgcct
     3061 tctaattttg attcttttga aatggtagat agaaagaaga tcagcacgga acagggaatc
     3121 gtatatcatt ttgaaggaac atttcgcgaa ggggatcaaa gacaaagagt agtcgggcga
     3181 ttgcgcatcc gcgatggaag agtgcatgcc atctattatc ttgcagacga caaagacttt
     3241 tcttctttcc gcgaaatcct ggaagatctg gatcaatcgc atcgatactt tcgcccttga
     3301 ttcttctttc cactcgcgcg aattacaaaa tcgattgaca aatcattaaa cggttgataa
     3361 ggacgagtcg gaatccctga tgggtcttcg gcgcctatag ctcaatggat agagcgcagg
     3421 tctccggaac ctgaaatcgg ggttcgaatc cctgtaggcg caaattggaa cttgtcctga
     3481 aatacattgg aatcggcctt ggagccgcac tcgctggctg gggaatgttt caccagctca
     3541 tcgtgggtgg ggccgtaagc atgatcaaac acccggatga cagcgaagcc aggctctttg
     3601 tcatgagctg ggtggcgcag ggtggtttca tgtcttttct aggagtcatt cccgcgactc
     3661 ttctattgat ctacggccca agcacagctg cggttcatac agttctttct ctttctgcgc
     3721 ttgcactcat cctcttatcc agccatgtat ttctcaccgg attcaaaact cacctcaggc
     3781 caattcaaat cggcgcaagc ctggaactga tttacgggat ttatctggtg atcctgatcg
     3841 ttctctcttg aaaattccag ttagtctacg ttcgctcaat cccacagaac tggcttcaca
     3901 ccacgcgaaa actcttcgga tttaagcc
//
LOCUS       DAOQJL010000372         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14168_length_3926_cov_5.017308, whole genome shotgun sequence.
ACCESSION   DAOQJL010000372 DAOQJL010000000
VERSION     DAOQJL010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14168_length_3926_cov_5.017308"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLG78_14395"
     CDS             <1..528
                     /locus_tag="PLG78_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24360.1"
                     /translation="AEALGIPMRKLYLPESPDMKEYESLMSRLVTDLVADQCTHAIFG
                     DIFLEDLRRYREEKLATQGLQGLFPLWKRDTSELIREFLDLGFKTMLVCVDSSLLDQS
                     FAGRIIDSDFLNDLPENVDPCGENGEFHTFVFDGPIFQSPIPVAKREVVYREYPAPQG
                     GKPIGFYFQDIETVP"
     gene            544..1611
                     /locus_tag="PLG78_14400"
     CDS             544..1611
                     /locus_tag="PLG78_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24361.1"
                     /translation="MNVRRHIFLFSLTLTACQGPTRLAEHWEVGELSVDGRTVFGSTL
                     DGIRSVGRDGSRGTLLVPYRTSVFEESQYRPGRIAVDDSYVYWTQREAPPGLDGVRRV
                     KKTGGSPESVMKVNSLAQSQPLHLQLDHAFVFVLTYGRGQFDNWIADTAGTYEGGSLV
                     RIDKKDLSVMDLASRVRAGSGLCVDSQFVYWSEGGTFHRTISGLADHVYNMDGKIYRV
                     RKNGGKPELLASGLDNPTELSISGGNIRFRSGRHYQKNSWTDDLGYFEIPTSGGDAKK
                     IAPYPYFEMGQNRYWLRIEYFGPANPGRRAHHFLEKESAAGKIERLYSTDDDISAFTL
                     DGGEFFWLQGTDYTLMKMKVR"
     gene            1679..2104
                     /locus_tag="PLG78_14405"
     CDS             1679..2104
                     /locus_tag="PLG78_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE24362.1"
                     /translation="MDSRRIIHLLSRTRDRIQKHFQHEIDGLKVGKIAPAHGSVLYAL
                     MDGPKAMQDLARLIERDTSTVTALADKLEKVGLAQRTVAPHDTRSYFMALTPKGKIAA
                     RKVIGASRGVVTRIFKGFSAAEREEFTRLLFRVYQNMEE"
     gene            2178..2564
                     /locus_tag="PLG78_14410"
     CDS             2178..2564
                     /locus_tag="PLG78_14410"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE24363.1"
                     /translation="MLEENKNLVREFFEAINRQDLARAEGMLSEQLSWWIIGRAKISG
                     QKDRRSVRISFKMVFRGFDHFAFTLHDITAEGNRVAVTAESRGRHKSGKDYNNHYHFL
                     FFIEDSQIMQVKEYFDTEHAMWLEAG"
     gene            2682..>3926
                     /locus_tag="PLG78_14415"
     CDS             2682..>3926
                     /locus_tag="PLG78_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE24364.1"
                     /translation="MIYRTQNPHTEIFEKLYDISSETTTDTTLRSAESASLVWSAMSF
                     SERAVRIRALADGLLRRKDEFAAVMAREMGKPIGQGLAEIEKSAVLIEFYAAKAESFL
                     SPEIIPSESSKSYACFDSLGVVFGIMPWNFPFWQVLRFAVPAWMAGNAAVFKPAPIVP
                     ECAVLLTSLCREFEVPLWHLFLEEKATAQVMTDPRISVISFTGSVRTGKLIAARAGLN
                     MKKGVYELGGSDPYLVLEDADVSRAASICASSRLINSGQSCVAAKRFLVHRSLKKSFE
                     EAFVAAMNVSYGDPLTNPALGPLARPDIRIHLADQVRSSVSQGAKLLLGGEIPDQKGY
                     YFPPTVLTDLSTEMAVWDEETFGPVAALHYFDSDEQAIALANDSDFGLAAAVFTADSA
                     RGESVALKLKVGSVSVNDLTRSD"
BASE COUNT          956 a          915 c         1105 g          950 t
ORIGIN      
        1 gcagaggcgc tcgggattcc catgcgcaaa ctctatcttc ccgagtctcc agacatgaag
       61 gaatacgaat cgttgatgtc gcgtctggta actgacctcg tcgcagatca gtgcacgcat
      121 gcgattttcg gagatatttt tctagaagac ctcaggcgct acagagaaga gaagctggca
      181 acgcaagggt tgcagggtct gtttcctctg tggaaaagag atacgtcgga actgattcgc
      241 gaattcctgg acctgggatt taagaccatg ctcgtatgcg tggattcatc gctactggat
      301 cagtcttttg cagggagaat cattgactct gattttttga acgaccttcc ggagaatgta
      361 gatccttgcg gtgagaacgg cgaatttcac acattcgttt tcgacggtcc cattttccaa
      421 tcgcccattc ctgttgcaaa gagggaagtc gtctatcgtg agtatcctgc acctcaggga
      481 gggaagccca taggattcta cttccaggac attgagacgg tcccatgagg aggaggatca
      541 gaaatgaacg ttagacggca tatctttctt ttttccctta cgctgaccgc gtgtcagggg
      601 ccaaccaggc tggcggaaca ctgggaagtt ggtgaactga gcgtggatgg aagaactgta
      661 ttcggatcca cactcgatgg gattcgcagc gtcggtcggg atggctcgcg gggtacgctc
      721 cttgtgccgt atcgcacttc ggtgtttgaa gagagccaat atcggccggg gcgaatcgcg
      781 gtggatgatt catatgtcta ttggacacag cgggaggcac cacccgggtt ggatggtgtg
      841 cgccgggtga agaaaacagg cggatctcca gagagtgtga tgaaagtaaa cagcctcgcg
      901 caatctcaac cgcttcactt gcagctagac catgcgttcg tgtttgtcct gacgtatggc
      961 cgcggtcagt ttgacaactg gattgcagat actgccggga cctacgaagg cggatctctg
     1021 gttcgaatcg acaagaagga tctgtccgtg atggatctcg cttcgcgtgt gcgcgccgga
     1081 tctggactat gtgtggattc gcagttcgta tactggtcag aaggtggaac gtttcataga
     1141 accatctccg gtctcgcgga ccatgtgtat aacatggacg gcaaaatcta cagagtaaga
     1201 aaaaacggtg ggaaacctga gttgctcgcg tccggtttgg acaaccctac cgaactgagt
     1261 ataagcggcg gaaacattcg ttttagaagc ggacgacact accagaagaa cagctggacg
     1321 gatgatctcg gatattttga aattccaacg tcaggcggcg atgcaaagaa aatagcgccg
     1381 tatccttact ttgagatggg tcagaacaga tactggttgc ggattgaata ttttggaccg
     1441 gccaatcccg gtcggagggc tcaccatttt ctcgaaaaag agagcgcggc gggcaagatc
     1501 gaaaggcttt actcaacaga cgatgacatc tccgcattca cactcgatgg tggggaattc
     1561 ttctggttgc agggcaccga ctacacactc atgaagatga aggttcggta acaagcgatc
     1621 attgcgggtt tgaaatcatc ttgacatagc ccgggtattt actttgatgt caaagtaaat
     1681 ggattccagg agaatcattc atctgctttc gcgtacgaga gaccgaattc agaagcattt
     1741 tcagcatgaa atcgatgggt tgaaagtggg caaaattgcg cccgcccacg gctcggtttt
     1801 gtatgcgctc atggacggcc cgaaggcaat gcaggatctt gcgcgcctga tagagcgaga
     1861 cacgtcaaca gtaacggccc tggcagataa gctcgagaaa gttggactgg cgcagcgaac
     1921 ggttgcgccg cacgatactc gttcctactt tatggcactc acgccaaaag gaaagatcgc
     1981 ggcgcgtaag gtcattggag cctcgcgcgg agtcgtgacc aggatcttca aaggtttttc
     2041 tgcagcggag cgcgaagagt ttacccgcct gctgttccgg gtttatcaga atatggagga
     2101 gtaatttttt atgcattagt ttgatgtcaa aataaaatag attccgattc gaaaagagcg
     2161 gaacaaagga gatgaatatg ctggaagaaa ataagaatct ggttcgcgag ttttttgagg
     2221 cgatcaatcg gcaagacctg gcgagggcag aagggatgct ttcggaacag ctcagctggt
     2281 ggatcattgg aagggcaaag atatcggggc aaaaggatcg tcgttcagtt cgcatttcgt
     2341 tcaaaatggt cttcaggggt tttgatcatt ttgctttcac gttgcatgac atcacagcgg
     2401 agggcaaccg ggtggcggtg accgctgaat cgcgggggcg ccacaaaagc gggaaagact
     2461 acaacaatca ttatcatttt cttttcttca tcgaggactc acagattatg caagtcaaag
     2521 agtacttcga caccgagcat gcgatgtggc ttgaggccgg ctaagctgtt gacggcgtga
     2581 catcaacgta acgctacgca tgcgatgatt caaaatgcag ctgacggcat ctgcaacagc
     2641 ggcaaaacgg cagtatcgca cttgcgatgg aaggacgagg gatgatttat cggactcaga
     2701 atccgcacac tgaaattttc gaaaaactat acgacatcag ctctgaaact acgacagaca
     2761 ccactctgcg ctccgctgaa agcgcttccc ttgtctggtc tgcgatgtcg ttttcagaac
     2821 gcgctgtacg catccgggct ctcgcagatg gactattgcg cagaaaagat gaattcgcag
     2881 cagtgatggc gcgcgaaatg ggcaagccca ttggccaggg actcgctgaa atagaaaagt
     2941 cagcggttct cattgagttt tatgccgcga aagcagaatc atttctttct cctgaaatca
     3001 tcccgagcga gtcttctaag agttatgctt gtttcgattc tctgggagtt gtctttggaa
     3061 tcatgccctg gaattttcct ttctggcagg ttctgaggtt tgctgttcct gcctggatgg
     3121 cggggaatgc ggctgtgttc aagcccgctc cgattgtccc agagtgcgcg gttcttctca
     3181 catcgctttg ccgcgagttt gaagttccgc tctggcatct tttcctcgaa gaaaaagcaa
     3241 cagcccaggt gatgaccgat ccgcgcattt ctgtgatatc ttttacgggc agcgttcgta
     3301 cagggaagtt gattgctgcg cgagccggtc tgaacatgaa gaagggcgtt tacgaacttg
     3361 gcgggtctga tccgtatctg gttcttgaag acgcggatgt atcgcgcgca gcgagcatct
     3421 gtgccagcag tcgactgatc aattcaggcc agagttgtgt cgccgccaag cgcttcctcg
     3481 tgcatcggag cttgaagaaa tctttcgaag aagcattcgt ggccgcgatg aacgtttcct
     3541 atggagatcc tttgaccaat cctgcccttg gacctctggc cagaccggac attcgcatac
     3601 acctggcaga tcaggttcgt tcgagcgttt ctcaaggcgc gaaactgctg ttaggtggag
     3661 aaattccaga tcagaagggg tattatttcc cgcccacagt gctcacagat ctttccacgg
     3721 agatggcggt ctgggacgaa gaaacatttg ggccagtggc cgctcttcac tatttcgatt
     3781 cagacgagca ggcgatcgct cttgcaaatg acagcgattt tggcctggcc gccgctgttt
     3841 tcactgcaga ctctgcaaga ggcgagagtg tggctctcaa gctcaaggtg ggcagcgtat
     3901 ccgtaaacga tctgacacga tcggat
//
LOCUS       DAOQJL010000373         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14239_length_3913_cov_4.128823, whole genome shotgun sequence.
ACCESSION   DAOQJL010000373 DAOQJL010000000
VERSION     DAOQJL010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14239_length_3913_cov_4.128823"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLG78_14420"
     CDS             complement(<1..1181)
                     /locus_tag="PLG78_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE24365.1"
                     /translation="MFPMLEKSILPEGRPPNIGALFLDQCHIYDESVALSFRDGSDVK
                     TVTYGKLAKIVRDIAGGVASRGLGPGARALVVAHNTPEWVFAFYAIHVCGGVDVCVNP
                     ALPHSVIIQLARQIKPAVALVEDPDLGELLEQEGKIKVIFLNWKKEGYKNLRSLRQRF
                     TFLGQGHKRRQPDHEASIVFKKSDPELQQPGGLILSHGSIIKNVEAYTSSLPGTQQDR
                     LMIRAPLWHSSGRLGMLTALSRGIELQLSRKDVLDDCEALQPTLLLAHPSTLDAIETR
                     VFEGGESGGMLGNLFMRAYIFFGAEAMRAMNHLTGQTAVFGSSEKQSFVGLVIDLVLL
                     IFLLPVKILGDGLFGTRLRKRFGGSLRCVLTGGAPVRGRLDLFFGMLGVSVLAAYFKT
                     EA"
     gene            1221..2234
                     /locus_tag="PLG78_14425"
     CDS             1221..2234
                     /locus_tag="PLG78_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE24366.1"
                     /translation="MMETEHHRIGQGQKPYLIAEIGLNHNGDLALAKKLVDAAGTSGA
                     HAVKFQLYDSDHFIHEEARLGDGSLREFFRQFELTPAQWRDLAGYSRSCGLDFFCSVF
                     DFPSIELYASLSPRLIKIASCDLTNRPLIQECAHRLPGVPLMIATGTADEAEVESLMA
                     WIRSLSSPVILLECVSHYPANPDEYNIGLIQQWNQKFDVPVGISDHTSGIGVSLAACL
                     LGAVAVERHFTTSHDLPGPDQKISLTAETFALMRNEIDAALSTRRSGNKQVQKSESAP
                     RKFGRRGVYAARDIKSGSTLSDQDLLFMRPGGDGVRPEENLAGRTLKKDKKTGDMILG
                     EDL"
     gene            2231..2767
                     /locus_tag="PLG78_14430"
     CDS             2231..2767
                     /locus_tag="PLG78_14430"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HNE24367.1"
                     /translation="MIQAQPSGMSEETRWYALYTMPQCEKRISLFLSKRKIDYYLPLV
                     PKKRKWSDRIKVVEFPLFPGYLFIHMPFYQRRLDVLLHPSALRFVQHEGRPAIIPDDD
                     IGYLHILVDNANDLQAHPEENLPEGQKVIVRSGPFKGIRGVVARVKNKDRIFVKLEHC
                     ALMASAEIDILDVEKLII"
     gene            2792..3760
                     /locus_tag="PLG78_14435"
     CDS             2792..3760
                     /locus_tag="PLG78_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNE24368.1"
                     /translation="MNPFSLFEKSYKMQIDELQRNASLIDTDSVTRAIDLIQNCKGKL
                     VLSGMGKSGLIAQKLAATFSSTGTPSFFLHPGEALHGDLGAVQKNDVLLVLAKSGESE
                     EVILMLQAIRKMGNPVISILGKSDSTAGRMSDVIIHASVSREADPLNLAPTASSTVSL
                     VVGDALASAVCELRGFKQENFALLHPAGQLGKRLLLKVEDLLILDRGLPVVGEHASMA
                     ELLEVESKPNLGGVMIADESGKLIGLITDGDIRRSILKFGDVLKCKIVDIMTRNPIHI
                     QTGTSALSALDLMENRPSPVSVLPVVDSDKKPVGLLRLHDLLRSGL"
BASE COUNT         1053 a         1036 c          965 g          859 t
ORIGIN      
        1 gcttccgtct tgaaatacgc tgccagaaca gaaacgccga gcatcccgaa gaacaaatcc
       61 agtctccctc gcaccggagc tccgccagta agcacgcagc gcaaactccc accaaaacgt
      121 tttcgaaggc gtgtgccaaa caggccatcc ccgagaatct tgacaggtaa aagaaaaatc
      181 agaagcacca ggtcaatgac aagcccgaca aaggattgct tttccgaaga tccaaacaca
      241 gctgtctggc ccgtgagatg attcatggca cgcatggctt ccgcgccaaa gaaaatgtac
      301 gcgcgcatga aaagatttcc cagcattccg cctgattccc caccctcaaa aacacgagtc
      361 tcaatggcgt ctaacgtaga tggatgagcc aggagtaaag taggctgaag tgcttcacaa
      421 tcgtccagaa catccttgcg cgaaagttgc agttcaatgc cacgggaaag ggcagtcaac
      481 attcccaatc ttccggagga atgccagagg ggtgcgcgaa tcatcaggcg atcctgctgt
      541 gtacctggca aagaagaagt gtatgcttcc acattcttga tgatagaacc atgggaaagg
      601 atgaggcctc cgggttgctg caattccgga tcacttttct tgaaaacaat actggcttca
      661 tggtccggct gcctgcgctt gtgaccctgc cctagaaacg taaacctctg cctgagcgat
      721 cttaaatttt tgtaaccttc cttcttccag ttcaaaaaga taactttgat cttgccctct
      781 tgctccagca gttctcccag atctggatcc tcaactaagg ccacggcagg tttgatctgg
      841 cgcgccagct gaatgatgac agaatgagga agagcaggat tcacgcacac gtccactcca
      901 ccgcacacat gaatggcgta aaatgcgaag acccattccg gcgtattgtg agcaaccacg
      961 agagcacgcg ccccggggcc cagacctcgc gaggccactc cacccgcaat gtcccttacg
     1021 attttagcga gcttgccata ggtgactgtc tttacatctg atccatcgcg aaacgaaaga
     1081 gccacggact catcgtaaat atgacattga tcgagaaaca gcgcgccaat gttggggggc
     1141 ctcccttcag gaagaatgct cttctctagc atcggaaaca tgcttgcgcg ggaggcgtgc
     1201 cgagtcaatc ggtacagcag atgatggaga cggagcatca cagaataggc cagggccaga
     1261 agccttacct catcgcagaa attggcctca atcacaacgg agatctggct ctcgcgaaga
     1321 aacttgtgga tgcggccgga acttcaggcg cgcatgcagt caaattccag ctctatgatt
     1381 cggaccattt catccacgag gaagcaaggc tgggggatgg atctctgcgc gaatttttcc
     1441 ggcaatttga acttacacca gcacagtggc gcgatcttgc tggttattct cgctcatgcg
     1501 ggcttgactt cttttgttct gtttttgatt ttccgtccat agagctctat gcaagtctct
     1561 ctccccgatt gatcaaaatc gcttcgtgcg atcttacgaa ccgtccgctg atccaggaat
     1621 gcgcgcatcg ccttcctggc gttccgctga tgattgcaac gggcactgcg gatgaagcag
     1681 aagtagagag tctcatggcc tggattcgct cactctcttc gccggtcatc ctgctcgaat
     1741 gcgtttcgca ttaccctgcg aatccagatg agtacaacat tggcctcata caacaatgga
     1801 atcagaagtt cgacgttcct gttggaatat cagatcacac ttccggaata ggagtgagcc
     1861 tggccgcctg ccttctgggt gctgtggcag tagaacggca tttcacgact tcgcacgatc
     1921 tgcccggccc ggatcaaaaa atttcgctga ctgctgagac tttcgccttg atgcgaaacg
     1981 aaattgatgc cgcactcagc acacgcagat ccggaaacaa acaggtacag aaatcagaat
     2041 ccgctcctcg caaattcgga aggcgtggag tctatgcggc gcgcgacatc aagagcggat
     2101 ccacgttaag cgatcaagat ttgcttttca tgagaccggg tggagacgga gtccgtccgg
     2161 aggaaaacct tgcggggcgc actctcaaga aagataagaa aaccggagac atgatcctcg
     2221 gagaggacct gtgattcagg cgcaaccttc cgggatgtcg gaagaaacca gatggtatgc
     2281 cctgtataca atgcctcaat gtgagaagag aatttctctg ttcctttcca agagaaagat
     2341 cgactactac cttcctctgg ttcccaaaaa aaggaagtgg tcagatcgca tcaaggtagt
     2401 ggaatttcca ctcttccccg gctacctttt cattcacatg cccttctatc agagacgatt
     2461 ggacgtactc ttgcatccaa gcgctcttcg ctttgtgcag cacgagggaa gaccggccat
     2521 cattcctgat gatgacattg gctacctcca tatactggta gacaacgcca atgatctcca
     2581 ggcccatccg gaagagaatt tgccggaggg acagaaagtc atcgtgcgat ccgggccctt
     2641 caaaggaatt cgcggagtag tggcgcgagt aaaaaacaaa gacagaattt ttgttaaact
     2701 ggaacactgc gcccttatgg cgagcgcgga aattgacatc ctggatgttg agaaactcat
     2761 catataacaa gccgtttcac gaaggtcgcc aatgaaccca ttttcactct ttgaaaaaag
     2821 ctacaaaatg caaatcgacg agctgcaaag aaatgcaagc ctcatcgaca ccgattctgt
     2881 tacgcgcgca atcgatctga ttcagaactg caaaggcaaa cttgtactca gcggtatggg
     2941 aaagtcagga ctcatcgcgc aaaagctcgc ggctaccttt tcatccacgg gcacaccatc
     3001 gtttttcctt catccaggag aagcgctcca cggagatctc ggagcagttc agaaaaacga
     3061 tgtgttgctt gtgcttgcga agagtggaga atccgaagaa gtaattttga tgctccaggc
     3121 aattcgcaaa atgggaaacc ctgtgatttc cattcttgga aaatcagatt ccacggcagg
     3181 aagaatgtca gatgtcatca tccatgcgtc cgtgagccgc gaagcggatc ctctcaatct
     3241 cgcgcccact gcatccagca ccgtctctct ggttgtagga gatgcacttg ccagcgctgt
     3301 ctgcgaactt cgtggattca aacaggaaaa ttttgctctt cttcatccgg cagggcagct
     3361 gggcaaacgg cttctcttga aagtggaaga tcttttgatc ctagatagag gcctgcctgt
     3421 cgttggcgaa cacgcaagca tggcagagct tctagaggta gagtccaaac caaacctcgg
     3481 cggagtcatg atcgcagacg aatcgggaaa actcattgga ttgatcacgg acggagacat
     3541 ccggcgatcc atcctcaaat ttggagacgt tcttaaatgc aagatcgtag acatcatgac
     3601 gcgaaacccg attcacattc aaacaggcac aagtgcgcta tccgcgcttg acctcatgga
     3661 gaaccgtccg tctcccgttt ccgttttgcc cgttgtggat tcagataaaa aacccgtagg
     3721 tcttcttcgc ctgcatgacc tgctgaggtc cggactataa catggcgcag gcgaaggccc
     3781 gaaaaaaaag caaatcagcc gtcagagcaa aacctgcggc aaaaaagagt gcgaaagctc
     3841 ccgcaaagaa ggctgcgaag aaaacgacag cgtggcccca ggcgtttcaa aaactaatcc
     3901 agaaatacgg aaa
//
LOCUS       DAOQJL010000374         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14262_length_3909_cov_4.416191, whole genome shotgun sequence.
ACCESSION   DAOQJL010000374 DAOQJL010000000
VERSION     DAOQJL010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14262_length_3909_cov_4.416191"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLG78_14440"
     CDS             <1..282
                     /locus_tag="PLG78_14440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE24369.1"
                     /translation="VARYGGEEFILILPETPLEGAAHVAEVIRAAIEAAQIEHKGSPL
                     GVVTASLGVAALLPEKGYEPVTLIQNADRALYQAKKERRNMVSVSREAL"
     gene            291..569
                     /locus_tag="PLG78_14445"
     CDS             291..569
                     /locus_tag="PLG78_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE24370.1"
                     /translation="MIEVTYTVPIERIDEVTAEHRAFLQKWYDEGTLLMSGPQVPRTG
                     GIILGKCESREKMEEIFSQDPFKLKGCAMYRIVEFNPVKHQEFLKTWL"
     gene            636..1313
                     /locus_tag="PLG78_14450"
     CDS             636..1313
                     /locus_tag="PLG78_14450"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE24371.1"
                     /translation="MRKLTIVFFLLCAFACKGPSQPDAGLFKNVQGDVTVERAGKVEP
                     AKVGDRFVPGDTVRTGENGIAIIAIKGTVGQAELQPNSVFRLNLGDQKTEVLLDKGNM
                     WLQVQKATSREEFTVRTPTTVAGVRGTKFYTFQNGDTVGVCYCEGDVDFAAGDSKSNT
                     YHKDTLVFTRNGKTVSLTADEVPGVTYEHNHSLMDGSPLGQQSKLTPEMKKKIFDAAE
                     KKFKELK"
     gene            complement(1314..2447)
                     /locus_tag="PLG78_14455"
     CDS             complement(1314..2447)
                     /locus_tag="PLG78_14455"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24372.1"
                     /translation="MGKRSALCAAFAVMAALSHCAARSECSALDISCSLPAYLLMLQS
                     RQFAYIANASTFEAFRYDQNTSQLTLLQTVASGQRCDAVRPHPYGVFLHCVVDVAGTD
                     YLRVFRIGSGGVLSFIEEFNIGSVTGLGLANHHRGDIVVLIVDTFSRYYLYQMDQITG
                     HLTLASQSPAGVGGAASAMSRDGNFIYGRTSGASQVTLRITGNSITQMQDLGNPGTNT
                     FTFTTTPDGFYQLQNINQALGIRRYTVNGDGTLSAPLDYPNSAQSEIFDDDSWTPDGR
                     FFYTRSGASADNLRGYAYSGGNINLVTGTPIVLPGGLGTPTVDPGGKYVFIDRSAAGL
                     YHVFQINRITGELTQMADSPRACTSNPCGRISFGTRARFPGDF"
     gene            complement(2490..>3909)
                     /locus_tag="PLG78_14460"
     CDS             complement(2490..>3909)
                     /locus_tag="PLG78_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE24373.1"
                     /translation="YWYTWQVTMLGLGPIWNGSNEEIKKETAKRLKEGAIFAFGLSER
                     AHGADLISSEMTLKTIAPGKYIANGRKYYIGNANKASFVSTFAKDAETGDYVFFAAES
                     AHPNYTCVQNVVNSQNYVAEYELKDYPITDKEILSRGRDAWDASLATIAYCKYNLGWA
                     SIGICTHAFYEAINHAANRVLFKHKVTDFPHIKQFMTDAWTRLLAMRLFSFRASDYMR
                     AASEDDKRYLLYNPMVKMKVTIQGEEVVNLLWDVIAAKGFEKDGYFEMAARDIRGLPK
                     LEGTAQVNMVLIMKFMDKFFFEPSPYPDLPRQKSPGNDSFMFAQGSTSKGQNRIQFHD
                     FNIAYNQSKLPNVKIFQSQIEVFKKFLKEAAPDKAQTRDLDFMLNVGELFTLVPYGQL
                     ILECAKSEGISDDLVDQIFDFMVRDFSRYALQMYSRTSSTQAQMDLCMQMIKKPAVNT
                     ERFERVWKENVLSLNMAYVMKP"
BASE COUNT          971 a          908 c         1008 g         1022 t
ORIGIN      
        1 gtagcgcgtt acggcggaga ggaatttatc ttgatcctgc ctgagacccc attggaaggt
       61 gcggcgcacg ttgcggaagt gattcgcgcc gccattgaag ccgcacagat tgagcacaaa
      121 ggatcccctc ttggtgtggt cacggcgagt cttggtgtgg cagctcttct tcctgaaaaa
      181 ggatatgaac ctgtaacgct cattcaaaat gcagatcgtg cgctctatca ggcaaaaaaa
      241 gaaagaagaa atatggtttc tgtttcgcgc gaggcattat gaagcatttc atgattgaag
      301 ttacatacac tgttccaatt gaacgaatcg atgaagtcac ggcagaacat cgggcgtttt
      361 tgcagaaatg gtatgatgaa ggcactcttt tgatgtctgg accccaggtt ccgagaaccg
      421 gcggcatcat tctgggtaag tgtgaatcgc gagagaagat ggaagagatt ttctctcaag
      481 atccattcaa gctcaagggc tgcgcgatgt atcgcattgt tgaattcaac cctgtaaagc
      541 atcaggaatt tctcaagacc tggctgtagt ctcttctttc cgcgttcgcg tttttattag
      601 ttgtgatttt ttgttctcta tctgaacttg ctctcatgcg taaactcacg attgtctttt
      661 ttcttctctg tgcctttgcg tgcaaaggtc cttcccaacc ggacgccgga ttattcaaga
      721 atgttcaggg agacgtgacc gttgaacgtg ccggtaaggt ggagcctgcg aaagtgggag
      781 atcggtttgt tccaggggat accgtacgaa ctggagagaa tggaattgca atcattgcaa
      841 tcaaaggaac tgtagggcag gcagagctgc agccaaacag cgtgtttaga ttgaatctgg
      901 gagatcaaaa gacagaggtt ctactcgata agggcaatat gtggctccag gttcagaaag
      961 caacttcgcg cgaagagttc acagtgcgca cgcctacgac tgtggccggt gtgcgcggca
     1021 cgaagttcta tacatttcag aatggagata ctgtgggcgt ctgttactgt gaaggtgatg
     1081 ttgattttgc cgcaggggat tctaagtcca atacctatca caaggacact ctcgtcttca
     1141 cgcgcaacgg caagacggtc agtctcacgg cggatgaagt gccaggcgtc acatatgaac
     1201 acaatcattc tctgatggac ggaagcccac ttggccagca gagcaaatta acacctgaga
     1261 tgaaaaagaa gatatttgac gcagcggaaa agaagtttaa agaacttaag tagttagaaa
     1321 tctccaggaa agcgtgcacg cgtcccaaac gaaattctgc cgcaggggtt ggatgtgcac
     1381 gctcgaggag aatcggccat ttgcgtcaat tctcccgtaa ttctattgat ctggaataca
     1441 tggtacaaac cagctgcgct tcgatcgatg aatacatact tgccgcctgg atccacggtc
     1501 ggggttccca ggcctccggg cagtacgatg ggcgttccag tgacgagatt gatgtttccg
     1561 cctgaatatg catagccgcg caagttgtcg gcggaagcgc cacttcgcgt gtaaaagaaa
     1621 cgaccatcag gagtccacga gtcatcgtcg aagatttcgc tctgcgcaga gttcgggtaa
     1681 tcgagaggag cagataacgt tccatctccg tttaccgtgt atctccgtat accgagcgcc
     1741 tgattgatgt tctggagttg atagaatccg tccggagtcg ttgtgaatgt gaacgtgttc
     1801 gttccaggat tgcccagatc ttgcatttgc gtaatgctat tccctgtgat tcgaagagtc
     1861 acctggcttg cgcctgaagt tcttccatat atgaagtttc cgtctctact catagcactg
     1921 gccgctccgc caacgccggc gggggattga gacgctagag tgagatgacc ggtgatctga
     1981 tccatctggt agagatagta tcgagaaaaa gtatccacga tcaagacgac aatgtcccct
     2041 ctgtgatgat tcgccagacc aaggcccgtt acagagccaa tgttaaattc ttcaataaaa
     2101 gagagaactc cgccgctgcc gattctgaaa acgcgcaggt agtccgtgcc tgccacatcc
     2161 acgacgcaat ggaggaagac accgtatgga tgggggcgca ccgcatcgca cctttggccg
     2221 gaagcgaccg tttgcagcaa tgtgagctgg gatgtatttt gatcgtagcg aaaggcttcg
     2281 aaagtggaag cgtttgcgat gtaggcgaat tgcctggatt gtagcatgag caagtatgca
     2341 ggcaagctac acgaaatgtc cagggcacta cattctgaac gcgcagcgca atgagagagt
     2401 gctgccatca ctgcgaaggc agcgcacaat gcgcttcgtt tgcccattgg gggctatacg
     2461 gcggacaagg tgaatctgac gcaatctttt tacggcttca tgacgtaggc catgttcaaa
     2521 gataatacat tctctttcca cacgcgctcg aatctttctg tgtttactgc cggcttcttg
     2581 atcatttgca tgcagagatc catttgcgct tgagtgctgg aagttctgga atacatctga
     2641 agcgcataac gtgaaaaatc gcgcaccatg aaatcgaaaa tctgatctac aaggtcgtct
     2701 gaaatccctt cgcttttcgc gcactccagg atcagctggc catagggaac gagtgtgaag
     2761 agctctccca cgttgagcat gaaatccagg tcacgtgttt gcgctttgtc cggtgctgct
     2821 tccttcagga acttcttgaa aacttcaatt tgcgattgga agatcttaac atttggaagc
     2881 ttagattggt tgtatgctat attgaagtcg tgaaactgaa tgcgattttg tcccttactc
     2941 gtgcttccct gcgcaaacat aaacgaatcg ttgccgggag atttctgacg cggcagatcg
     3001 ggataaggag atggttcaaa gaaaaactta tccatgaact tcatgatcaa taccatattg
     3061 acctgcgcgg ttccttctag tttaggcaat ccgcggatgt ctcgcgcagc catttcaaaa
     3121 taaccgtctt tctcaaatcc ttttgccgca atcacatccc atagcaggtt cacgacttct
     3181 tctccctgaa ttgtgacttt cattttcacc atagggttgt agagaagata gcgtttgtcg
     3241 tcttccgacg ccgcacgcat gtagtcgctg gcgcgaaatg aaaatagtct catcgcgagc
     3301 aagcgagtcc acgcatccgt catgaattgt ttgatgtgtg gaaagtctgt gaccttgtgt
     3361 ttgaaaagaa cacgattggc tgcgtggttg attgcttcat aaaatgcatg agtacagatc
     3421 ccgatggacg cccatcccag gttgtatttg cagtaagcaa tggtggcaag ggatgcgtcc
     3481 caggcgtcac ggcctcgcga tagaatttct ttgtccgtaa tcgggtaatc cttaagctca
     3541 tactcagcta catagttctg ggagttcact acgttttgca cacacgtgta gttcgggtgt
     3601 gcagattctg ctgcgaagaa cacgtagtct ccggtttccg catcctttgc gaaagtggaa
     3661 acaaaagagg ctttgtttgc atttcctatg tagtacttac gaccatttgc aatgtacttg
     3721 ccaggggcaa ttgttttgag agtcatctcg cttgagatga gatccgcacc gtgcgctcgc
     3781 tcagataggc cgaacgcgaa aatcgctcct tctttgagcc tctttgccgt ttcctttttg
     3841 atttcctcat tgcttccgtt ccagataggt ccgagaccta acatggttac ctgccaggta
     3901 taccagtac
//
LOCUS       DAOQJL010000375         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14344_length_3897_cov_4.220979, whole genome shotgun sequence.
ACCESSION   DAOQJL010000375 DAOQJL010000000
VERSION     DAOQJL010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14344_length_3897_cov_4.220979"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLG78_14465"
     CDS             complement(<1..1116)
                     /locus_tag="PLG78_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24374.1"
                     /translation="MSFVWLRLSARESRLLTLLLGAASAVPFSTIAYIYGLSTNVIFW
                     ILCLWAGCGFILRLYSWRPARFFRRIRKSFVFHAIWIACAAILLAPRLAGGSQFAVYQ
                     ANRWDTFGYLESASSYSRYSFDFVEHATLADQLADPLLVQGQIQMFVRPSIHMFYGVV
                     SHFLPGEFWDLHYTFLLSFVLLSTFACAFFLSYLFRVKSIYGMIAGAAFSLGFWGQYS
                     LDINAWSQVASTGVLVTLTALLLRLRMRKLNLRTAILISLFSAGSLYLYPEAFVVVGA
                     CSALSAVVSALWLRNPRTLILPAVGGTMGVAAGFLFFHGTIQFILTQLLYSKTSAVDW
                     WKIYQAFFLGRDLIDFDPVLLQKSAREIFSLLPAKSIF"
     gene            complement(1167..1586)
                     /locus_tag="PLG78_14470"
     CDS             complement(1167..1586)
                     /locus_tag="PLG78_14470"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE24375.1"
                     /translation="MKDILLRAFHNTKLRFLFAGGSTAVIYFGLLYLLHDMMGQSSNL
                     SVGTAYFVSTAYHFLCNKFFVHRDDSLDTAHLQLGKYLALIAVNYAITWVVTNGILVM
                     GGNVYLGVCASVLITLVFTYVVMRRAIFRRGSEPREQ"
     gene            complement(1555..2274)
                     /locus_tag="PLG78_14475"
     CDS             complement(1555..2274)
                     /locus_tag="PLG78_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE24376.1"
                     /translation="MTPSVAILAGGLATRIRPITDKIPKALIPIRGRPFVDYQLELLA
                     GMNLSRVVLCTGYKGEMIEDHVGDGSAFGVSVQYSHDGETLLGTGGALKRALPLLGDR
                     FIVIYGDSYLDADYRPIVEFFDTQSSASHRPQALMTVFRNEGLFDRSNVVFRGGRIIV
                     YDKKQDHPEMNYIDWGLGILHAGVFAPFSERSVFDLAELYGHVLSEGALLGYEVKQRF
                     FEIGSVSGIEEFERHIASRLP"
     gene            complement(2271..3251)
                     /locus_tag="PLG78_14480"
     CDS             complement(2271..3251)
                     /locus_tag="PLG78_14480"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24377.1"
                     /translation="MIIVRSPLRISLGGGGTDLASYYLDHGGFLIAAAIDRYVYITLH
                     ETFIDEIIVKYSKLERVSSADQIEHPIIRECLKLLDIKRTNIELTSMADIPAGTGLGS
                     SGSFTTALLKTLHTYNKNIITTHELAEQACHVEIDRLKEPIGKQDQYIAAYGGITCFE
                     FRKDHSVKASALDLSEETRLDLEDNLLLFFTGYSRSASSILKEQDDKSKQNNADMIQN
                     LHFIKDIGKQSKTALETGNLHEFARLMDVHWQEKKKRSSGMSNQKINEWYDFAMQNGA
                     LGGKLIGAGGGGFLMFYTENKTKLRHAMMSLGLVEVRFRFDFEGTKVVIS"
     gene            complement(3248..>3897)
                     /locus_tag="PLG78_14485"
     CDS             complement(3248..>3897)
                     /locus_tag="PLG78_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE24378.1"
                     /translation="RVRAKCGFNHRFHPAMLKAKELLESNDCGELMFVRGRYGHGGRP
                     GYDREWRADPEISGGGELLDQGMHLIDLSRWFLGDLPRVQGIARTFFWDMKVDDNAFM
                     LLETEKGQVAQLHVSWTEWKNLFSFEIYAKRAKLHIEGLGGSYGLERIAFYPMKPEMG
                     PPDSLIYEFPGADLSWKREWKNFKASIEKGEPLLGSLHDALAALEIVDTIYRRKS"
BASE COUNT         1108 a         1008 c          935 g          846 t
ORIGIN      
        1 aaatatagat ttcgcaggca acaatgaaaa tatctctcgc gcactcttct gcagaagaac
       61 cggatcaaag tcgatcagat cgcggcccag aaagaaagcc tgatagatct tccaccagtc
      121 aacagcagaa gttttcgaat acagcagttg agtcagaatg aattgaatcg ttccgtgaaa
      181 gaaaagaaat ccagccgcaa ctcccatggt tcctcccact gccggaagga tcaacgtgcg
      241 cggattgcga agccagagag cggagacaac tgcagagagc gcagaacatg cacccactac
      301 tacaaaagcc tccggataaa gataaagaga tcccgccgaa aatagagaga tcagtattgc
      361 cgtgcgcaga ttcaattttc gcatgcgaag acgaagaaga agagctgtca gagtcacaag
      421 cacgcccgtt gacgcaacct gactccaggc gttgatatcc agagaatatt gtccccagaa
      481 acccagtgaa aatgcagcgc cggcgatcat gccgtagatt gacttcacgc gaaacaaata
      541 ggaaagaaag aatgcgcagg caaaagtaga aagcaaaaca aatgaaagca gaaaagtata
      601 gtgcagatcc cagaattctc ccggaagaaa gtgggacacc acgccgtaga acatatggat
      661 ggacggacgc acaaacatct ggatctgtcc ctggaccagc aacggatcgg ccagctgatc
      721 ggctaacgtt gcatgttcaa cgaaatcaaa agaatacctg gaataagaag aagcagattc
      781 cagatagcca aaggtgtccc agcgatttgc ctggtagact gcaaattgac tgcctcccgc
      841 aagtctcggt gcaagcaaga tcgcagcgca tgcgatccag attgcatgaa aaacaaatga
      901 tttgcgaatc cttctaaaga aacgggccgg cctccaggaa tacaatctca gaatgaaacc
      961 gcagcctgcc cacaggcata gaatccagaa tatcacattc gtgcttagcc cgtatatata
     1021 ggcgatcgta gaaaaaggaa cggcagatgc agcgcccaga agcagcgtca gaaggcgcga
     1081 ttctctggca gacaggcgaa gccagacgaa ggacatgcct atccccgcaa aaagaaaaat
     1141 aagaaagcta aaaaagagaa tctgattcat tgttctctgg gctcagaacc ccggcgaaag
     1201 atggcccggc gcatcacaac gtatgtgaac acaagcgtaa tcaacacgga tgcgcaaaca
     1261 cccagatata cattcccgcc cattacaaga attccgttgg tcacaaccca ggtaattgcg
     1321 tagtttacgg cgatcaaggc aaggtattta ccaagctgca ggtgcgccgt atccagagaa
     1381 tcatctctgt gcacaaagaa cttgttgcaa agaaaatgat acgcagtcga aacaaagtat
     1441 gctgtcccga cggacaggtt gctgctctgc cccatcatgt catgaagcag atacaacagt
     1501 ccaaagtaaa tgacggccgt acttccgcct gcgaagagga aacgcagttt ggtattatgg
     1561 aaggcgcgaa gcaatatgtc tttcaaattc ctcaatcccg gaaacagaac caatttcgaa
     1621 aaagcgctgt ttgacttcat aacccagaag cgcaccttct gaaagaacgt gtccgtacag
     1681 ctccgccagg tcaaacacag agcgctcgct gaatggtgca aaaacgccgg cgtgcaaaat
     1741 accaagcccc cagtcgatgt agttcatctc cggatgatct tgctttttat cataaacgat
     1801 gattctgccg ccgcgaaaca caacattgct ccgatcaaac aagccttcat tgcgaaatac
     1861 agtcatgaga gcctggggcc gatgcgaagc agatgattgc gtgtcaaaaa attcaacaat
     1921 cggacgatag tcggcatcga ggtaggaatc gccgtaaatg acaatgaatc gatctccaag
     1981 aagaggaagt gcacgtttga gcgcgccgcc agtccccaga agtgtttctc cgtcgtgact
     2041 gtattgaaca gagacaccaa aagcggaacc atctccaacg tgatcctcga tcatttcccc
     2101 tttgtagcct gtgcacagca cgacgcggct tagattcatg ccggcaagca gttcaagctg
     2161 gtaatctaca aacggacgtc cgcgaatcgg gatcagtgct tttggaattt tgtccgtaat
     2221 tgggcgaatg cgagtggcca gaccgccagc aagaatggca accgaagggg tcacgaaata
     2281 actaccttcg ttccttcaaa atcaaaacgg aagcgcactt ccacaagccc gagactcatc
     2341 atcgcatgac ggagtttagt tttgttttca gtgtagaaca tgagaaaccc gccgccgccc
     2401 gcgccgatca gcttaccgcc aagtgcaccg ttctgcatgg cgaagtcata ccactcattg
     2461 atcttctgat tgctcatgcc gcttgatcgt ttcttttttt cctgccagtg tacgtccatg
     2521 aggcgtgcga actcgtgcag gttgcctgtc tcaagcgcag tcttgctctg ctttccaatg
     2581 tcttttatga aatgcagatt ctgaatcata tccgcattgt tctgtttgct cttgtcgtcc
     2641 tgctccttga gaatcgaaga agcagacctg gaataaccgg taaagaaaag aagtaaatta
     2701 tcttcaagat caagtcgcgt ctcttccgaa agatccagcg ccgatgcctt tacggaatga
     2761 tccttgcgaa attcaaaaca ggtgatgccg ccgtaagcgg caatgtactg gtcctgcttt
     2821 ccgatcggct ctttgagtcg gtcgatctcc acgtgacagg cctgttcggc gagttcgtgt
     2881 gttgtaatga tgttcttgtt gtaggtgtgc agagtcttca gaagcgccgt cgtgaagctt
     2941 ccggaggaac ccagtcccgt gccggccggt atgtctgcca tactcgtaag ctctatgttc
     3001 gtgcgcttga tgtctagaag cttgagacat tcgcggatga tcggatgctc gatctggtct
     3061 gctgaggata ctcgttccag ctttgagtac ttcacaatga tttcatcaat gaaagtctcg
     3121 tgaagagtga tgtagacgta gcggtcaatc gcggcggcaa tcagaaagcc gccgtggtcc
     3181 agatagtaag aagcgaggtc tgttcctcct cctccgagtg aaatccgcaa cgggctgcga
     3241 acaatgatca tgattttctc cgatagattg tatctacgat ttcaagtgct gcaagcgcat
     3301 cgtgcagcga gcccaacaga ggttcgccct tttcaatcga cgccttgaag tttttccatt
     3361 cgcgcttcca cgacagatcc gcgccaggaa actcatagat gagagaatcg ggcggcccca
     3421 tctccggctt cataggataa aatgcgatgc gctcaagacc gtaactgcct ccgagacctt
     3481 cgatatgaag ctttgcacgc ttggcataaa tttcaaatga gaacagattc ttccattctg
     3541 tccagctgac gtgaagctgc gccacctgcc ctttttctgt ttcaagaagc atgaacgcat
     3601 tgtcgtcgac tttcatgtcc cagaagaaag tgcgcgcaat cccttgcacc cgcggaagat
     3661 cccccaggaa ccagcgcgaa aggtcaatca aatgcatccc ttgatcgagc agctcgccgc
     3721 cgcctgaaat ctcgggatca gcacgccatt cccgatcata gccaggccgg ccgccgtgac
     3781 catagcgacc ccgcacaaac atcaattcgc cgcaatcgtt tgactcaaga agctcctttg
     3841 ccttgagcat cgcaggatga aaacgatgat tgaatccgca tttcgcgcgt actcttt
//
LOCUS       DAOQJL010000376         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14494_length_3874_cov_3.323121, whole genome shotgun sequence.
ACCESSION   DAOQJL010000376 DAOQJL010000000
VERSION     DAOQJL010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_3874_cov_3.323121"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLG78_14490"
     CDS             complement(<1..280)
                     /locus_tag="PLG78_14490"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24379.1"
                     /translation="MFRGFDLRSQAIVLLCLLTVSPALSGESAAKLYQDGMQALSRGD
                     RENARYYFRRALEVNPGYREAALADGDLNLQMGDLKRARESYDRMLSVD"
     gene            complement(264..2300)
                     /locus_tag="PLG78_14495"
     CDS             complement(264..2300)
                     /locus_tag="PLG78_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNE24380.1"
                     /translation="MSHRAAILSALLLLLSPMLAQRLPSFRIKEEESRDHFRRGLTFY
                     NSGQYLAAREFFYKALNRVPSFHLARRYLGDANYYSGEWNSALEQWEFLDKRSEGTYS
                     LIRMRSDLLRFRLNGFRDAGPYIHFRTFNKKDFGDISALRPVDIAVDSEANVYYLFSE
                     SANIIVSNAGATSFRQIRGSIFDRMKGPSAMALLRDRIYVADYSDDRIRIFDRSGSSI
                     SSFGTTGSEKGQLRGPAGIAVTADAVYVSDSGNRRIEKFDLDGKHILSFLTADTGLKF
                     PAGIAVDGENVYVADRDSARIFTFDRDGNQLDQIESKMLSHPRGVSVGAGKLVIADEE
                     AGVLFYDLAEKTWTRLETRTEDDRTLVWKRAYSARMDSNGVLMAADTGAGLVHMFMPE
                     ALRISNLECRVERVDTASFPDVAVFLQLRNRLGNPLPGLDRNEIRIFENDRFLRGINT
                     SSMEDYQNRVNLSFVKENNADLSSEENKNVLSTILTSFLAPIRISDRIKVIRAGESVR
                     SVYEGLQRRMIIRSMQEGETTERPDVGKALVEAIGGQITSLGPRAVIAIVSGASANPF
                     VQYPVDRVEYFARAQGIPIYIISFEEGGPGDTQDTYRALATNTGGAYIRAYDETSVRA
                     LYESIRARKDERYIITYHSGLTPDLADRYVDVRIDVDHQGMTGSAEGGYFVPRL"
     gene            complement(2404..3243)
                     /locus_tag="PLG78_14500"
     CDS             complement(2404..3243)
                     /locus_tag="PLG78_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24381.1"
                     /translation="MKSQKIWHVSEGRKFPLDLKKHDTLSFQIMEISPNDLRTLHPEP
                     DDLHVFLIQGERGGLAAMRNLMESTPHLSDFPRIIILPQDQYGLYEANPRNMNLLVLD
                     DSVRPGHLKLILETVLRVEHYRQIVFHMSEQMREQAGLLDKMLGLARDEIKNARSESA
                     AFEALMDFETVHKRFERSIADAMERTMRLKDQELLEMKSTLAAYEHLSEFRDRELSET
                     RKQMHATESVLDLSHRENMEREKIIQALERLRMLTDKELIDLFEENQTLRSRLGMEPR
                     LFP"
     gene            complement(3258..>3874)
                     /locus_tag="PLG78_14505"
     CDS             complement(3258..>3874)
                     /locus_tag="PLG78_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNE24382.1"
                     /translation="EQFQAMMTLTVQVEVFLKDAEKTSRKFKVEIQNRFAKSVACMLF
                     FFISFPLGLVVKRSGRGMSFSLALVVFVAYFGLYLYGTGLGESGKAHPAIGAWLPDVV
                     VAFIGVYVMASRTEGFKQRFRALVSNAKRKWDTIVWSRLSPVLMPVFTPVWKAIRRVG
                     SLVARILGPVARPVAGVVRRSVNFLRSSIFAPLAKRLLAFRNRS"
BASE COUNT          938 a         1050 c          942 g          944 t
ORIGIN      
        1 cgtccacgga tagcatccga tcataacttt cgcgggctct cttcaaatct cccatctgca
       61 gattgagatc cccatctgcg agggccgctt ctctatagcc tggattcacc tccagggcgc
      121 gcctgaaata ataccgtgcg ttttctctgt cgcctcgcga aagggcctgc atgccgtcct
      181 gatacagttt ggccgcactc tccccagaaa gcgcaggcga cacggtcaaa agacagagca
      241 aaacaatggc ctgcgatcgc agatcaaagc cgcggaacaa agtaacctcc ctccgcggac
      301 ccggtcatgc cctggtgatc tacatcaatt cgcacatcta cgtaacgatc cgcaagatcg
      361 ggggtcaaac cagaatgata tgtaatgata tacctttcgt cttttcgcgc gcgtatgctt
      421 tcatagagag ctcgcacaga agtttcgtcg taagcgcgaa tgtaagcgcc acctgtgttt
      481 gtcgcaaggg cgcggtaggt atcctgtgtg tcccccggcc caccctcttc aaaagaaata
      541 atgtagatgg gaatgccctg cgcgcgcgca aaatactcta ctctgtccac gggatactgc
      601 acaaatgggt tggcgcttgc gcccgaaaca atagcaatca cagcgcgagg accaagagaa
      661 gtgatctgtc caccaatggc ttccacgagc gccttgccga cgtctggcct ttctgtcgtt
      721 tccccttcct gcatgctacg aatgatcatg cgtcgctgca gaccttcata cacggatcgc
      781 acgctttctc ctgcgcgaat gactttgatt cggtctgaaa tgcgaatcgg tgcgagaaat
      841 gacgtcaaaa tcgtgctcag aacatttttg ttctcttcgc tggatagatc tgcgttgttt
      901 tctttcacaa aggatagatt cacgcgattc tgatagtcct ccatgctgga ggtattgatg
      961 ccgcgtagaa aacgatcatt ctcaaaaatg cgaatctcat ttcgatccag acccggaagt
     1021 ggattcccga gcctgtttct cagctgtaaa aacactgcca cgtcggggaa agaagcagtg
     1081 tccactctct ccaccctgca ctccagattg ctgatgcgga gcgcttcagg catgaacatg
     1141 tgaaccaggc ccgctcctgt atctgcggcc atgagaactc catttgaatc catgcgcgca
     1201 gaatacgctc tcttccacac aagagtgcga tcgtcctctg ttctggtttc cagtctggtc
     1261 cacgtctttt ccgcaagatc atagaagagt acacctgctt cctcatctgc gatcacaagt
     1321 tttcctgccc cgacggacac gcctcttgga tgcgagagca tttttgattc gatctgatcg
     1381 agctgattgc catcgcgatc aaacgtgaaa atgcgagcag aatctctatc agccacgtaa
     1441 acgttttcgc catccaccgc aatgccggcg ggaaacttaa gccctgtatc tgcagtcaaa
     1501 aacgatagta tatgttttcc atctaaatca aatttttcaa ttctacgatt tccactgtcc
     1561 gaaacataca ctgcgtccgc tgtcactgca attccagctg gtcctcgcag ctgccccttt
     1621 tcgcttccag tggtaccaaa tgaagaaatg ctcgatcctg atcgatcaaa gatgcgaatg
     1681 cgatcatcag aatagtctgc aacgtaaatg cgatctctca aaagtgccat cgcagacggt
     1741 cctttcatac gatcaaagat tgaaccgcga atctgacgaa acgaagtggc tcccgcattc
     1801 gatacgatga tgttggcaga ttcgctaaac agatagtaga cgttcgcttc gctgtcgacg
     1861 gctatatcca cgggcctcag cgcggagatg tctccaaaat cttttttatt gaatgtgcga
     1921 aagtgaatgt atgggccagc atcgcggaat ccattcaggc gaaagcgcag gagatcgctt
     1981 cgcatacgaa tcaaagaata cgtaccttct gatcgcttat ccagaaactc ccattgttcc
     2041 agagcgctat tccattcacc cgagtaatag tttgcatctc cgagatagcg ccgcgcgaga
     2101 tgaaacgacg gaacacgatt gagagctttg taaaaaaatt ctctggcagc caggtactga
     2161 ccggaattgt aaaatgtaag gccacggcga aaatgatcgc gcgattcttc ttccttgatg
     2221 cgaaaggaag gcagcctctg tgcgagcatc ggcgacagga ggagcaacag tgcgctcagg
     2281 atcgctgccc tatgtgacat aatccagcgt gaagtctgga atcgcttcct tcaagccttt
     2341 tttaggccta ttttctcccc tgccacctgg actggcctcc gacccggagt gacggcgaag
     2401 gcctcagggg aagagtcgag gctccattcc cagcctcgaa cgcagggtct ggttctcctc
     2461 aaatagatcg atgagttctt tgtcagtgag cattcggagc ctttcgagtg cctggatgat
     2521 cttctccctt tccatgtttt ccctgtggct cagatccagg acagactcgg ttgcatgcat
     2581 ttgtttacgg gtttcggaaa gttcgcggtc gcgaaactcg ctcaaatgct catacgcagc
     2641 cagagtgctc ttcatttcga gcagctcctg gtccttgagt cgcatggtgc gttccatggc
     2701 gtccgcgatg gatctctcga aacgcttgtg gaccgtctca aaatccatca aagcctcaaa
     2761 tgcggcggac tctgagcggg cgtttttgat ttcatcacgc gcaaggccga gcatcttgtc
     2821 gagaagcccc gcctgctctc tcatttgttc ggacatatga aatacgatct gacgatagtg
     2881 ctctactcga agcactgttt cgaggatcaa tttcagatga cctggacgaa ccgaatcgtc
     2941 caggacaagc aggttcatat tccgtggatt ggcctcatag agtccatatt gatcctgcgg
     3001 tagaatgatg atacgcggga aatcggaaag gtgtggagtc gattccatga gatttcgcat
     3061 ggctgccagg ccgccccgtt cgccctggat gaggaatacg tgaaggtcgt ctggctccgg
     3121 gtgcagagtt ctcaggtcgt tggggctgat ctccatgatc tgaaagctca gagtgtcgtg
     3181 cttcttgaga tccaggggaa actttctgcc ttccgatacg tgccagattt tctgcgactt
     3241 catagcctgt gtacagttca ggagcggttg cggaaggcaa gcaaacgctt ggccagagga
     3301 gcaaatattg atgagcgaag aaagttcaca cttcgcctta cgaccccggc gaccggacga
     3361 gccaccgggc cgagaattcg ggccacgaga ctgccgaccc ggcggattgc cttccagacc
     3421 ggggtgaaca ctggcatcaa gacaggagac agacgcgacc atacaatcgt gtcccacttt
     3481 ctttttgcat tggacacaag tgcgcggaag cgttgcttga atccttccgt tcgagaggcc
     3541 attacgtata cgccaatgaa cgctaccact acatcgggaa gccatgctcc gatggcagga
     3601 tgagctttgc ccgattctcc aagccctgtt ccgtacagat acaaaccaaa gtacgcaaca
     3661 aacacaacga gtgccagaga gaagctcatc cctcttccag atctttttac aaccaggccc
     3721 agtggaaatg aaatgaaaaa gaacagcatg cacgcaacgg atttcgcaaa acgattctga
     3781 atctctactt tgaacttccg gcttgttttt tctgcgtcct tgaggaatac ttctacctgc
     3841 actgtaagag tcatcatcgc ctggaattgt tctt
//
LOCUS       DAOQJL010000377         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14500_length_3873_cov_4.836564, whole genome shotgun sequence.
ACCESSION   DAOQJL010000377 DAOQJL010000000
VERSION     DAOQJL010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_3873_cov_4.836564"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1024
                     /locus_tag="PLG78_14510"
     CDS             224..1024
                     /locus_tag="PLG78_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNE24383.1"
                     /translation="MWRDPRHYQLGALYSLFLYGAFALGFDVKPLHVVLTIATAVTTQ
                     VLFSWIFRVPVELRSALITSCSLCLLLRSSVWWVPVFAAFVAMGSKFVLRWNGKHIFN
                     PANGAIILCLLVFDNAWVSPGQWGNTAFFGFLFACLGGLVVMRAKRADVSLAFLFFYS
                     AMIFARSLYLGEPMTIPIHRLESGALLLFTFFMISDPKTTPNSRVARILYAFLVAAGA
                     YYVTFKMFRTNGLLWSLVIVSLLVPFMDLLFRGEIYQWRKKDEVQFSS"
     gene            1002..2312
                     /locus_tag="PLG78_14515"
     CDS             1002..2312
                     /locus_tag="PLG78_14515"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNE24384.1"
                     /translation="MKFSSAVKYSTVALAIVTSVEVVYAFCGFYVAKADTKLFNQASS
                     VVLVRDGDRTVLTMSNDFQGDPKEFALVVPVPTFLERGQINVGDPAVIAHVDAFTSPR
                     LVEYFDSDPCMVEYERDDVRRAEVPSANTQGPGRHNDLGVKVEAKYTVGEYDILILSA
                     KESGGLETWLKQNGYKIPPGASEVLGSYIKQNMRFFVAKVNLKEKAKTGFTYLRPLQI
                     AFESPKFMLPIRLGTVNANGPQELFVFAITKQGRIETTNYRTVRLPTDINVPEYVKSE
                     FADFYKSMFAEMVRKEDMRAVFLEHAWDMSWCDPCAADPLSPAELKKLGVFWVKESEG
                     GAVPAFVTRLHVRYDRAHFPEDLVFQQTGDKANFQARYIMQHPFKGATSCPAGERYKS
                     QLVVRQNQEAENLAKLTGWDIEAIRKKIGIMKATERKWWQNLWD"
     gene            2325..3674
                     /locus_tag="PLG78_14520"
     CDS             2325..3674
                     /locus_tag="PLG78_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HNE24385.1"
                     /translation="MRTLYIECVSGIAGDMFASAFLDAGLISRQEVEDLPRRIGWPDV
                     KIEVSSVVRATMKATSIDVSAQGESWKQSLKESASAPIPSGKIEIRGKAHEATHSLSA
                     RPVHSDGGAGSPLHNHMHGDSHGHTHGHSHGGTHANADGWHTQYADLDRFLATRDLPI
                     GVIEKARSIFYNLGRAEAEAHGMAIEYVAFHEVGAYDSIIDVLLAAICIDRIGVDQVE
                     CSSIPVGRGMIQIAHGLQPVPPPASANLLRGMPVAPVPIAIQKENVELATPTGLAILK
                     TLEPRFRWDWPSGILENAGMGAGHMDFPGFPNVVRIGVFSSGVAADSSNYAKETVCQL
                     TFNVDDQTGEETGRFIDRAMKEGALDIQVIPATGKKGRPSMVIQLLAREEQKDRMIDL
                     VLRSTRSFGVRVEKVERYRLETRFEERDVQGSKVKYRIGTNLAGAFIKEKPEFDDLP"
     gene            3683..>3873
                     /locus_tag="PLG78_14525"
     CDS             3683..>3873
                     /locus_tag="PLG78_14525"
                     /inference="COORDINATES: protein motif:HMM:NF026306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4505 family protein"
                     /protein_id="HNE24386.1"
                     /translation="MREYFYRIDSRGRVIHDGTEISDEGFLNFFFERLRDNDTGLHSD
                     FSFVSPCGNEMNFIKPDLFP"
BASE COUNT          915 a          871 c         1072 g         1015 t
ORIGIN      
        1 ctgagcgtcg aggcgaacga ggatttcgcg gtccggtccg tgctgatctg ctttgcgaag
       61 aaacgtgagt agaacgagca gtagaattcc gcaagtggcc agaacgttaa aaacgatgag
      121 aagttcggtc atggtcagca tgataccgtg tcactgtgtc agaaatgatc gtttttcata
      181 ttttttttga acgagaacgt agtttttttt gtccattctt cgtatgtgga gagatccaag
      241 acactatcaa ctgggcgcgc tttattcttt gtttctatat ggtgcctttg cgctggggtt
      301 tgatgttaag ccgttgcatg tggttttgac gatcgcaact gcggtgacta ctcaggtgct
      361 tttcagttgg atttttcgag tccctgtgga gctgcgaagt gctctgatta catcttgttc
      421 tctctgtctt ttgcttcgct ccagtgtctg gtgggttcca gtgtttgccg cctttgtggc
      481 gatgggaagc aagttcgtgc tccggtggaa cggaaaacac atcttcaatc cagcgaatgg
      541 tgcaatcatt ctgtgcttgc tcgtgtttga caacgcgtgg gtttcaccgg gccaatgggg
      601 caacacagct ttcttcggat tcctgtttgc atgtcttggc gggctcgttg tcatgcgtgc
      661 gaaacgtgcg gacgtaagtc ttgcattcct attcttttac tccgcgatga ttttcgcaag
      721 gtctctgtat ctgggtgagc caatgacgat tccgatccac agactggaaa gcggcgccct
      781 tcttttgttt acattcttta tgatttcaga tccgaaaaca acgcccaatt ctcgcgttgc
      841 cagaattcta tacgcgtttc tggttgccgc gggagcatac tacgtgactt ttaagatgtt
      901 tcgcacgaac ggtcttctct ggtcgcttgt gatcgtttcg cttctggtgc cattcatgga
      961 tttgctattt cgcggtgaga tttaccaatg gagaaagaaa gatgaagttc agttcagcag
     1021 ttaagtattc caccgtcgcc cttgcgattg ttacttctgt ggaagttgtg tacgcgtttt
     1081 gcggcttcta tgtggcgaaa gcggacacga agttgttcaa tcaggcgtcg agcgtggtgc
     1141 tggttcgcga cggagataga acggttctta ctatgtccaa tgactttcag ggagacccga
     1201 aggaatttgc acttgtggtt ccagttccta cgtttcttga gagaggccag atcaatgtag
     1261 gcgatcccgc cgtcatcgct catgtagatg cgttcacttc cccaaggctg gtggagtatt
     1321 ttgattctga tccatgcatg gttgaatatg aacgcgatga cgtgcgaaga gcagaagtgc
     1381 cgtccgcaaa tacgcaagga cctggaagac acaacgacct gggtgtaaag gtcgaggcta
     1441 agtataccgt gggagaatac gatattctga ttctatccgc gaaggaaagc ggcggtctcg
     1501 aaacctggct gaagcagaac ggatataaaa ttccacccgg cgcctcagag gtcctgggaa
     1561 gctacatcaa gcaaaatatg aggttctttg ttgcgaaggt gaatttgaag gagaaagcaa
     1621 agaccggatt tacatacctt cgtccgcttc agattgcatt cgaatcgcct aaattcatgc
     1681 tcccgatccg actgggcacc gtgaatgcaa atgggccaca ggaattgttc gttttcgcaa
     1741 tcacaaagca ggggcgcatt gaaaccacga actatcgcac agtccgactt cctactgata
     1801 tcaatgtgcc ggaatatgta aaaagcgaat ttgccgattt ctataagtcc atgtttgctg
     1861 aaatggttcg caaagaagac atgcgcgcgg tttttcttga gcatgcgtgg gatatgagct
     1921 ggtgcgatcc atgtgccgcc gatcctctct ctcccgcaga gctgaaaaaa cttggagtat
     1981 tctgggtgaa ggaatccgag ggtggggctg ttcctgcctt tgtgactcgg ctgcatgtcc
     2041 gctacgaccg tgcgcatttt ccggaggacc tggtattcca acaaacgggt gataaggcga
     2101 actttcaagc tcgctatatt atgcagcatc cgtttaaagg tgccacctct tgcccggcag
     2161 gagagcgtta caaatcacag ctcgtcgttc gtcagaatca ggaagctgag aaccttgcaa
     2221 agctcaccgg ttgggatata gaagcgattc gcaagaagat cggtatcatg aaggcgacag
     2281 agagaaaatg gtggcagaat ctctgggatt aatcatcccg gagcatgcgc actttataca
     2341 ttgaatgcgt ctccggaatt gccggagaca tgttcgcgtc tgcgtttctg gacgctggat
     2401 tgatttcgcg acaagaagtg gaggatcttc ctcgaaggat cggttggcca gatgtaaaga
     2461 tagaggtcag ttcagtggtg cgcgccacca tgaaagctac atcgatcgat gtatcggcgc
     2521 agggcgaatc gtggaagcaa tcgctcaaag aatccgcgag cgcccccata ccctcgggca
     2581 agatagaaat tcgcggcaaa gctcacgagg caacacattc gctttcagcc cgcccggttc
     2641 attcagatgg gggggcgggt tctccactgc acaatcatat gcacggagat tcgcatggac
     2701 atactcatgg ccattcgcac gggggcactc acgcaaatgc agacggatgg catacgcaat
     2761 atgcggatct ggatcgattc ctggccacgc gcgacctacc gataggcgtc attgagaagg
     2821 ctcgtagcat cttttataac cttgggcggg cggaagcgga agctcatggg atggcaatcg
     2881 aatacgtagc attccacgaa gtgggcgctt acgacagcat catcgatgtg cttctggcag
     2941 ccatttgtat agaccgtatt ggagtggatc aagtggaatg ttcgtccata ccggtaggac
     3001 gcgggatgat tcaaatcgct catggtttgc agcctgtgcc tccaccggcc agtgcgaatc
     3061 ttcttcgcgg tatgccagtg gctcccgttc cgattgccat tcagaaagaa aatgtggagc
     3121 ttgcgacgcc tacggggctt gcgattttga aaaccctgga accccgcttt cgctgggatt
     3181 ggccgagcgg aattctggag aacgcgggaa tgggtgccgg gcatatggac tttcctggtt
     3241 ttccaaacgt cgttcgcatt ggtgtgtttt ctagtggtgt tgcggccgat tcttccaact
     3301 atgcgaagga gacagtatgc cagctaacgt ttaatgtgga cgatcaaacg ggagaggaaa
     3361 ccggacgatt cattgatcgc gcaatgaagg aaggtgcgct ggacattcag gtgatacctg
     3421 cgaccggaaa gaagggcaga ccttctatgg tgattcaatt gcttgcacgt gaagaacaaa
     3481 aggacaggat gatcgatctt gtccttcgca gcacacgttc ctttggtgtt agggtggaaa
     3541 aggttgagcg ttatagattg gagacccggt ttgaagagcg ggacgtgcag ggtagtaaag
     3601 taaagtatag aatcggaacg aacctggctg gagcattcat aaaagaaaag ccggagtttg
     3661 acgacctgcc gtagttcagc atatgcggga gtacttctat agaattgatt cgcgtgggcg
     3721 agtgattcat gacggcacgg agatttcgga tgaaggattt ctcaatttct tcttcgagag
     3781 actgcgcgac aacgatacgg gactccacag tgatttttca tttgtctctc cttgcggcaa
     3841 tgaaatgaat ttcataaagc cagatctttt tcc
//
LOCUS       DAOQJL010000378         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14503_length_3873_cov_3.350183, whole genome shotgun sequence.
ACCESSION   DAOQJL010000378 DAOQJL010000000
VERSION     DAOQJL010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14503_length_3873_cov_3.350183"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLG78_14530"
     CDS             complement(<1..880)
                     /locus_tag="PLG78_14530"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE24387.1"
                     /translation="MDKIIKVGNRSIGGDSTFLIGDIGSNHNQDIQLAYDIIDAGVEA
                     GLDAVKFQSLQRKHLYLNPTQEETDLYNKIDLEEDWHFKLKEHCDRRGIVFFSSPTYF
                     RSVDLLEQAGVLIYKLASAQIGSFPQIVRRVASLRKPVILSTGLVSYSGLERVIKIFQ
                     EEGNDQFIILHCNSLYPPAYDRVNLGLISTYRRMFSNPVGFSDHTMENYATVAAVALG
                     AKVIERHFCMDRAIVTPDSPVSLNPAEIKDMVKAVRSIEQALGNKPRTTIEPEEAGFL
                     KSISYRLVLTRDFEKGH"
     gene            complement(881..1369)
                     /locus_tag="PLG78_14535"
     CDS             complement(881..1369)
                     /locus_tag="PLG78_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24388.1"
                     /translation="MFEITLKTCISLDNYYRGGVDLLDVVKAIEKKIDAISPSIVYTH
                     FPEDLNVDHRIVSRAVMTACRPVPRQLVREIYFFDVLSSTEWGTGARAFQPNVFVPLT
                     GGKGGNYLDAKLRALAFYETEMRAFPHARSLETVKALVALRGSNIGESAAEAFEAARV
                     VR"
     gene            complement(1369..3141)
                     /locus_tag="PLG78_14540"
     CDS             complement(1369..3141)
                     /locus_tag="PLG78_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC12162 family protein"
                     /protein_id="HNE24389.1"
                     /translation="MPRDRFLITTADERAWRRDVPVVFLGEWCRLFKRKEAWSELDAI
                     VCPPYGVSKARKDHDFEAASGLERELIPVLAQGLNRAHGLHHSERFWRILAGHWLKRF
                     VDTIYNRYHTLEQCTSDVSGMRVLWDEQYSLAVVNSNSFVWASNDAVWNNVLFARLLP
                     YLGSFQIERVLCDLGSGFRMKEVHPAHALPATALSRLKARLRNILRWLRAKRFSNRDA
                     LILHTYMSAEAEFDLQRKIGQRPRTWHVPPHAALAPVDRNLRESLRSDASVADPLRRS
                     LHDLLFDLLPVCFLEGFSDLRHQASMLPWPRHPRFIFNCANYDTDEAFKCWTAMRVEE
                     GIKYFIGQHGNNYGTHRQIARRTTEQMTADKFLTWGWTDGHPAKVPAFMFRHGGAAKV
                     TMDPHGGLLLAEDCLPHHVYCWDIFHDYDRLFSAQLSFARALPLALKAKLTVRLHGPG
                     ENLERWGDRERWRDFDPEIELEGGAAPFGELLRRSRLVVYGYDSTGMLENLASNVPTM
                     AFWADGLEHVLDTALPVYALLVDAGIIHLTPESAAAKVAEVWDDVSSWWLSPSVQEAR
                     SKFCEAFARTSETPVDDLVRLLTA"
     gene            complement(3131..>3873)
                     /locus_tag="PLG78_14545"
     CDS             complement(3131..>3873)
                     /locus_tag="PLG78_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNE24390.1"
                     /translation="CVVVDSYLADRSKYEILRREFSRVVAIDDYNRIVYPADLIINPN
                     VYFSSVDYSSQSAVCVGGPCYIILRGLFRAARPSSGPTGGVLVTLGGSDCRRLLSRFG
                     SLSETLPLTLIVPEEAARPELKRLFPLARVLGRLDEADMLDNFLRARVVVPGCGQSLH
                     ELAYLAKNTIGIWIDDDQTGNCDYYVASGFLKQKITWDDPHLEDKVTELALKFLSSHE
                     QRTRPAFDPERNMENYFRHLALKDNHAA"
BASE COUNT          999 a         1050 c          963 g          861 t
ORIGIN      
        1 tatgcccttt ttcaaagtct cgagtcagca cgaggcgata tgaaattgat tttagaaatc
       61 cagcctcttc cggctcgata gtcgtgcgcg gtttattgcc cagggcctgt tcgatcgagc
      121 gcactgcttt caccatatcc tttatctcag cgggatttaa agagacagga ctgtccggag
      181 tgacgattgc acgatccatg caaaaatgcc tttcaattac tttcgcacca agagcaaccg
      241 cggcaactgt cgcataattt tccatggtat gatctgaaaa cccgacagga ttggaaaaca
      301 ttcggcgata ggtagaaatc aatcccagat tcacgcgatc gtacgctggt ggatacagac
      361 tgttgcaatg aaggatgatg aattgatcgt ttccttcttc ctggaaaatc ttgattacgc
      421 gttcaagacc ggaataagat acaagaccgg tggaaaggat cactggcttt cgcagggagg
      481 caacgcgccg aacaatctgt ggaaaagaac caatctgcgc agatgcaagc ttgtaaatga
      541 gcacgcccgc ctgctccagg agatcgacag accgaaagta agtgggtgaa gaaaaaaata
      601 caattccgcg gcggtcgcag tgctctttca gtttaaaatg ccagtcttct tccagatcaa
      661 tcttattgta gagatctgtt tcctcctgtg ttggattcag atacagatgc tttctctgga
      721 gtgactgaaa cttcactgcg tccagccctg cttccacacc cgcatcaatg atatcataag
      781 caagctggat gtcctggttg tggtttgagc caatgtcccc gatcaggaat gtactgtctc
      841 cgccaatcga gcgattgccg actttaatga tcttgtccaa ttatctaacc accctggcag
      901 cttcgaatgc ttctgctgcc gactcgccta tatttgaacc acgcagcgcg acgagagcct
      961 taaccgtctc cagggaacgc gcatgcggaa acgccctcat ttcagtttca tagaaagcaa
     1021 gtgcgcgcaa cttcgcgtcg aggtagttgc cgcccttccc gcctgtcaaa ggcacaaata
     1081 catttggctg aaacgcgcgt gcaccagtgc cccattcagt gcttgaaagc acatcgaaaa
     1141 aataaatctc acgaacgagt tgtctcggca cgggtctgca tgcagtcatc acggcccggg
     1201 acacaatgcg atgatcgacg ttcaaatctt ctggaaaatg cgtataaaca atagaagggg
     1261 aaatcgcatc gatcttcttt tcgattgctt tgacgacatc gagtagatcc acgccgccgc
     1321 ggtaataatt gtccagggaa atgcaggtct tcaaggtgat ctcaaacatt atgcggtcag
     1381 aagtcgaacg agatcgtcga caggtgtttc gctcgtgcgg gcgaaagcct cacagaattt
     1441 acttcgtgct tcctgaacgg aagggctgag ccaccaggaa gaaacatcat cccagacctc
     1501 cgccactttc gcagcagcag attcgggggt aagatgaatg attccagcat cgactaacaa
     1561 cgcgtatacg ggaagagctg tgtccagaac atgctccaga ccatctgccc agaacgccat
     1621 cgtgggcaca ttgctcgcca gattttcaag cataccggta gaatcatacc catacactac
     1681 gaggcggctg cggcggagca gctcgccaaa cggtgccgcc cccccttcca gctcgatctc
     1741 aggatcaaaa tccctccacc gctcacgatc tccccagcgc tcgaggttct cccccggacc
     1801 gtgaagccgc actgtgagtt tcgccttcag agccagtggc agagctcgcg caaaagaaag
     1861 ctgagcagag aacagacgat cgtagtcatg aaagatatcc cagcaataga catggtgcgg
     1921 cagacaatct tcggcaagca agaggcctcc gtgcgggtcc atggtaactt tcgcagcgcc
     1981 tccatgacga aacatgaacg ctggaacctt cgccggatgg ccgtctgtcc atccccatgt
     2041 tagaaatttg tccgcagtca tctgctccgt agtgcgccgg gctatctggc gatgcgtgcc
     2101 ataattgttt ccatgctgtc caatgaaata cttgattccc tcttccactc gcattgcagt
     2161 ccagcatttg aaagcttcgt ctgtgtcata gtttgcgcag ttgaagatga atcgcgggtg
     2221 tcgcggccag gggagcatag atgcctgatg acgaagatcg gaaaaaccct cgaggaagca
     2281 gactggcagc agatcgaaca acaaatcgtg caaagagcgg cgtaaagggt ccgcaacgga
     2341 agcatcgctt ctcagacttt cgcgcagatt ccgatcgact ggagccagcg cagcatgagg
     2401 aggtacatgc catgttcgcg gtctctggcc gatttttctc tgcagatcga actctgcctc
     2461 cgctgacatg tacgtatgaa ggatgagtgc gtcgcgattc gaaaatcttt tcgcacgcag
     2521 ccacctgaga atgtttcgaa gccgggcctt caacctggag agcgcggtcg ctggaagcgc
     2581 atgcgcagga tgaacttcct tcatgcgaaa tcccgaacca agatcacaga gtacgcgctc
     2641 tatttgaaat gaacccagat aaggaagcag ccgcgcgaac aacacattgt tccagacggc
     2701 atcattcgat gcccatacga aagaattaga attgaccact gccagcgagt attgttcgtc
     2761 ccagagcaca cgcatccctg atacatcgga ggtgcactgc tccagggtgt ggtatcgatt
     2821 gtaaattgta tcaacaaatc gcttcaacca gtgcccggca aggatccgcc agaatctctc
     2881 gctgtgatgt agtccatgag cccggttgag tccctgggcc agaacaggaa tcagttcacg
     2941 ttccagtcct gatgccgctt caaagtcgtg atccttgcgc gctttgctga ctccataggg
     3001 aggacacaca atcgcgtcca gttcgctcca ggcctctttc ctcttgaaca gcctgcacca
     3061 ttctccaaga aagacgacag gcacgtcccg cctccacgcc cgctcgtctg cggtagtaat
     3121 caggaatcta tcacgcggca tggttgtctt ttagagccag atgacggaag tagttctcca
     3181 tatttctctc aggatcaaag gcagggcgag tgcgctgctc gtgcgaagag agaaatttca
     3241 aagcaagttc agtcaccttg tcttccagat gcggatcatc ccaggtaatt ttctgcttca
     3301 gaaaacctga cgcaacgtag tagtcgcaat ttcctgtctg gtcatcatct atccagattc
     3361 caatcgtgtt cttggcaaga tatgcaagct catgaagaga ctgaccgcat ccgggcacta
     3421 ccactcgagc gcgcaagaaa ttatccagca tgtctgcttc gtcaagacgg ccaagaactc
     3481 gagcaagtgg aaacaatcgc ttcagctccg ggcgggccgc ttcttccgga acaatcagag
     3541 tcagaggcag ggtttcagaa aggcttccaa accgggacaa cagacgccgg cagtcagagc
     3601 cgcctaacgt tacaagtact ccgccggtcg gcccggagga tggtcgagcg gcgcgaaaca
     3661 gcccgcgcag aatgatgtaa cacgggccgc ccacgcacac agcgctctgg ctgctgtagt
     3721 ccacagacga aaaatagaca ttcggattga tgatcagatc cgcgggatac acaatacgat
     3781 tgtagtcatc gatagcgact acacgcgaaa actctctgcg aagaatttca tatttgctcc
     3841 ggtccgcaag atacgaatct acaacaacgc agt
//
LOCUS       DAOQJL010000379         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14602_length_3859_cov_4.544164, whole genome shotgun sequence.
ACCESSION   DAOQJL010000379 DAOQJL010000000
VERSION     DAOQJL010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14602_length_3859_cov_4.544164"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLG78_14550"
     CDS             complement(<1..900)
                     /locus_tag="PLG78_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24391.1"
                     /translation="MRWLLRMARWPGGIERVFLGRAPDTDPLKTCYVWSRKSALDELA
                     LIHFLLNRPMRRGSFVAKNWPREPFASPHKVSGVVSRLGEASSLFSSLSRFFPEAPDT
                     AEDPAADDALDIPDDSNLAETILCADQEPHLRGRIREPSMPDNVARDYSLKLVPLSVT
                     GDFLCPDLLGRSDLWVHPGSPIHPPGNTAEKPHLNHLLRMSLQKGLPLLATHLISIAL
                     FEFRLEHKPFRMDKVVTRFHDLARRVRRLDFPHDERLDHNEFRNSRMNRFLRRFEKWF
                     YLVDSKYFFKTLEYPEVELGRPMN"
     gene            complement(1041..1361)
                     /locus_tag="PLG78_14555"
     CDS             complement(1041..1361)
                     /locus_tag="PLG78_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNE24392.1"
                     /translation="MNLKQIEQTLTQKRAELAGRADRLQQDITRQSSPLEEDWEEASI
                     QRENDQVLDALDDETRAELQQIDRALGRIREGKYGQCEKCSGPIAAERLEALPYATTC
                     VKCA"
     gene            1469..2329
                     /locus_tag="PLG78_14560"
     CDS             1469..2329
                     /locus_tag="PLG78_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE24393.1"
                     /translation="MIRIAAFGDIHGSFDDEDLRMIQHSDYDMVLIVGDLPGRTHAGT
                     LDVARRLARMPRPAYFMPGNHDGVSLIQLIAELQGNHALVERSHRRQFKLVENLRAAL
                     GPVSYCAYSLHEINIKGTAISLVSARPHSMGGTSFSYRPYLEKEFGISDMNQSAEKIK
                     RLLDEARSPVLVLAHNGPAGLGSKRDDIFGCDFRKEEGDFGDPDLADALDYAEGAKIQ
                     VLGVVAGHMHHRIRGGGERVTIAKRKNTLCVNCARVPRIFKEDGQTLRHHVRIEISEK
                     GMEVEQVTRP"
     gene            2326..2967
                     /gene="lipB"
                     /locus_tag="PLG78_14565"
     CDS             2326..2967
                     /gene="lipB"
                     /locus_tag="PLG78_14565"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNE24394.1"
                     /translation="MTQRIKLITRIGKPLAYQEYLHLESDLRARREELILVCEHSPVI
                     TAGVQFKDKSLRVPQTELAPRGVAFYKTSRGGDVTAHEPGQIVIYVHVDLKARSLDVS
                     KFFKGLLESAQTAAKNSFGLDLDLNTEAPGLYVSGRKIASIGIQFKSFFTGHGLAINV
                     TNDLSTFSLIDPCGFDSLEMTSVEKEGGRVDKTGLFIDLFYEHLQKNHLPERA"
     gene            3063..>3859
                     /locus_tag="PLG78_14570"
     CDS             3063..>3859
                     /locus_tag="PLG78_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE24395.1"
                     /translation="MQKAVWFVESHFRETVTLEEIAQTCGVSAYHLTRTFATSMGIPL
                     MRYVRARRLSEAARQLIQTKANIVDVALETGYGSHEAFTRAFRDQFDLTPEQLRAQGH
                     ANNITLVEALSMHSSTMTRIQPPRLESEGPRIIAGLIQRHDCSNPAGIPEQWQRFSKW
                     IGNIPGQIGDAAYGINYNFDEEGTFDYMCGVQVSGESTIPSDLKVLKLPAQTYAVFEH
                     AGHIAGIRGSFTSIWNWFSASDLKPTEAPNLEKYGRQFDPATGLGGL"
BASE COUNT         1026 a          961 c          989 g          883 t
ORIGIN      
        1 gttcatgggc ctgcccagtt ccacttccgg gtattcgaga gtcttaaaga aatacttaga
       61 atccaccaga tagaaccatt tctcaaatcg tctcaagaat cgattcatgc gcgaattgcg
      121 aaactcattg tgatctaacc tttcgtcatg cggaaaatcc agtcttcgca cgcgacgagc
      181 cagatcgtga aaacgcgtaa ctaccttatc catgcgaaat ggtttgtgtt ccagtctgaa
      241 ttcaaaaaga gcaatggaaa tcaaatgagt ggccagcagt ggaagaccct tctgcaagga
      301 catgcgaagc aggtggttga gatgaggctt ttctgcagtg tttcccggcg gatgaatggg
      361 agacccggga tgaacccaca gatcacttct tcccagaaga tcggggcaaa gaaaatctcc
      421 tgttacactc agaggaacaa gtttcaagct atagtcccgc gcaacgttat ccggcataga
      481 gggctcgcga attctgcccc gaagatgtgg ttcctggtct gcgcaaagaa tggtctctgc
      541 cagatttgaa tcgtcaggaa tatcgagagc atcatctgcc gcaggatctt ctgccgtatc
      601 aggcgcttcg ggaaagaatc gactcaggct tgagaaaagg gaagaagcct ctcccagacg
      661 agagacaact ccagaaactt tatgtggaga ggcgaatggt tcccggggcc aattttttgc
      721 aacgaagctc ccgcgacgca taggacgatt gagcaaaaaa tgaatgagag caagctcatc
      781 gagcgcggac ttgcggctcc agacgtagca ggtttttagt ggatctgtat ccggcgcgcg
      841 accaaggaaa acacgttcta tcccaccggg ccatctggcc atccgcagaa gccagcgcat
      901 ggcgcgattt tgagaagaat ctttataata aaatgcaact gattccggca aaatccggat
      961 cggcaaaaat cctccggttc ttttttgcac ggccatagac aacgagacgg gtcatccagt
     1021 gccgtcgcaa gcaggaaacc ttacgcgcac tttacgcagg tggtggcgta gggcaatgcc
     1081 tccaggcgtt cggccgcaat tggtccggaa catttctcac actggccata ttttccttct
     1141 cggatgcgac ccagggcccg gtcaatctgt tgcagctctg cgcgagtttc gtcgtctaga
     1201 gcgtccagca cctggtcgtt ctctctctgg atgctcgctt cctcccagtc ctcttccagg
     1261 ggagaagact gccgcgtgat gtcctgttgc aatctgtctg cccgaccggc cagttcggcc
     1321 cttttttgag tcaaagtttg ttcgatttgc ttcagattca ttgctttact ctgactcaga
     1381 atcgcagatc ttacaatgac atttcttagg aagcaattat gacgatcaac agcgttgacg
     1441 gcgaaaaagc ggatgaggat gaatgcacat gatccgaatc gcagcctttg gcgacatcca
     1501 cggcagcttc gatgacgaag acctgcgcat gattcaacac tcggattatg atatggttct
     1561 gattgttggg gaccttccag gcagaacaca tgctggaaca ctggatgtgg ccagacgcct
     1621 cgcacggatg cctcgacctg cctatttcat gcccggaaat catgacgggg taagcctcat
     1681 tcaactcatc gcagaattgc aggggaatca cgctctcgta gaacgatctc atcgcaggca
     1741 attcaagctg gttgaaaatc taagagcggc actcgggccc gtttcctatt gtgcttattc
     1801 tctgcacgaa atcaacatca aaggaacggc catctctctc gtatcagcca gaccacacag
     1861 catgggcggc acctcttttt cctacagacc gtatctagaa aaagaattcg ggatctctga
     1921 catgaatcag tcagccgaaa aaatcaaacg attgttagac gaagccagat cccccgtgct
     1981 ggttctcgca cacaacggac ccgcaggcct tggttcaaaa agagatgaca tttttggttg
     2041 cgactttcgc aaagaagaag gcgatttcgg agatccagat cttgcagatg ctctggatta
     2101 tgcggaagga gcaaagatcc aggtccttgg cgtcgtggca ggccacatgc accacagaat
     2161 tcgcggcggt ggtgaacgcg taaccattgc taagcggaaa aacacgttat gcgtcaactg
     2221 cgcgcgcgtt ccgagaatct ttaaagaaga tgggcaaacg cttcgccatc atgttcgaat
     2281 tgaaatttcc gaaaagggaa tggaagtaga acaggtgacc aggccgtgac ccaaaggatc
     2341 aaactgatta cgcgaattgg aaaaccgctt gcctatcagg aatatctcca tctggaatct
     2401 gatctgcgcg cgcgaaggga agaactgatt ctagtctgcg agcacagtcc agtcatcaca
     2461 gccggcgttc aattcaaaga caaaagcctt cgtgtgccgc aaactgagct tgcgccacgg
     2521 ggggtggcct tttacaaaac ttcccgcggt ggggatgtta cggcgcatga accagggcag
     2581 atcgtcattt acgttcacgt ggatctgaag gcaagaagcc tggacgtttc caaattcttt
     2641 aaaggattgc tagaaagtgc gcagacggcc gccaaaaatt cgttcggact ggatctggat
     2701 ctaaacacgg aggcaccagg actatacgtg agtggcagaa agattgcatc cattggaatt
     2761 caattcaaat cgttttttac gggacacggt ctggcgatca acgtcaccaa tgacctttct
     2821 actttttctc tgattgatcc gtgcgggttt gattccctcg aaatgacgag cgtcgagaaa
     2881 gaaggcggca gagtagataa gaccggactg tttattgatc tcttttacga gcacttacag
     2941 aagaatcacc ttccagaacg agcgtgattc aaaaaaaaga ataattcgca agaacgatca
     3001 agaacaccga acgggggctg tgttatgatt catcagcatc aagaggcatt caattgaatt
     3061 tcgtgcaaaa agcagtctgg ttcgtggaga gtcacttccg ggaaaccgta accctggagg
     3121 aaatcgccca aacttgcggt gtttccgcgt atcacctgac tagaactttt gctacatcca
     3181 tgggaattcc tctgatgcga tatgttcgcg cacggcgact gagtgaagcg gcaaggcagc
     3241 tcatacagac aaaggcaaac atcgtggatg ttgctctcga aactggttac ggctcgcacg
     3301 aagcgttcac tcgagccttt cgcgatcaat ttgatcttac gcccgaacaa cttcgtgcgc
     3361 agggtcacgc aaacaacatc acgctagtgg aggcattgag tatgcacagt tcaactatga
     3421 ccagaatcca accacctcgc ctggaaagcg aaggccctcg catcatcgca ggattgattc
     3481 agagacacga ttgctccaat cctgccggaa ttccggagca atggcaacgc ttcagcaagt
     3541 ggattgggaa tattcccggg cagataggag atgctgcgta cggcatcaat tacaacttcg
     3601 atgaggaagg aacgttcgat tacatgtgcg gagtgcaggt gagcggcgaa tcaactatcc
     3661 cgtcggattt gaaggtattg aagctacccg cgcagacgta tgccgtgttc gagcatgcgg
     3721 gacacattgc cggtattcgc ggaagtttca cttccatatg gaattggttt tccgcttccg
     3781 acctgaagcc aacggaagca cccaacttgg agaaatacgg cagacaattt gatcctgcaa
     3841 ccggactcgg aggtctgga
//
LOCUS       DAOQJL010000380         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14795_length_3824_cov_4.222605, whole genome shotgun sequence.
ACCESSION   DAOQJL010000380 DAOQJL010000000
VERSION     DAOQJL010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14795_length_3824_cov_4.222605"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLG78_14575"
     CDS             complement(<1..182)
                     /locus_tag="PLG78_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24396.1"
                     /translation="MKKRLPTLTASILGLLIIAGLTAEEKRISHADVARSVMAVYEKM
                     GSYSASFQITSQDGNNA"
     gene            362..2146
                     /locus_tag="PLG78_14580"
     CDS             362..2146
                     /locus_tag="PLG78_14580"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE24397.1"
                     /translation="MITTEGLAYLTPNLAAILLTLALCIRCWKSQSLPGHKELAFALI
                     AELLWMCGHIGKLTSPTLEGKIIWDDIEFLGTCAWPMATFAFALKFSGARLPRILHAG
                     LWILPLFIAGAALTDPWTAYLHPNARLVNTTPFTGLLYDFKLILSVTFLQILLQVTLA
                     AVLIIARSFWLTTSQRVQSMILSAGILLPVIGSLLILLGIQFTEHRDTSHITFGVGNV
                     LIYFGLFGISKEAEHRFSTLFDNASDAILIMNGLMIIDCNIKAQTIFQASKEKLVGTS
                     VIDACAPVQEERTCEPLWRANVEAAMSGQPQFFGWTFVRKTGEEFDAEVSLTCTQVGP
                     DMLLQAFVRDRSEIRAAALERESLIRDLRAKNIELERFNYSVSHDLKSPLITIKGFVG
                     MLAQDVEKQNYANVPNHIHRISSAADKMYSLLEDLLALSRAGKATSAPALNEMDEILD
                     ESLGALEGLIQSRSAEIIRKERLPLIFADAMRMKAVWQNLIENSIKYARHGVPPKIQI
                     GCKADAQGYAFYVQDNGQGIDPRYQLQVFGQFHKLDASTEGSGIGLSLVQRIMEAHQG
                     RAWVESDGVGRGATFWFHLPRTPKASDG"
     gene            2143..2409
                     /locus_tag="PLG78_14585"
     CDS             2143..2409
                     /locus_tag="PLG78_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24398.1"
                     /translation="MSETLCDFCNEVQIYTGTNATTNYLPDALRSVMYRKNLKSGEIL
                     RCPACNRAYFFDERTPGGSDDAMTTTFYQDFYPLTAEELSALER"
     gene            complement(2406..3245)
                     /locus_tag="PLG78_14590"
     CDS             complement(2406..3245)
                     /locus_tag="PLG78_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24399.1"
                     /translation="MDYLIRITETDSLTPGDVIRRVGDGKEQQGCFVRRDESGGLIAG
                     DVVDLVNFSFVTEVGMLRSGSDFEFFKHKHRFSTSSVAEAARAIITGWVLFKEHLDLQ
                     KSILSFVESVYSPEHIMELSRDARLQALFVPVQQKFKIGKFKEKIDWEKVRDQRFRDA
                     LEALVGGKHMTYLAFIPGEQSHKPVFYTIGTKPHQETNVSLQREPFAFKPTHGGHIKI
                     VSDESDKVKRFIVDAGSNFIGVGSHTSLATAEMITNALHELFPQHEFTALAGRGAHGA
                     QSY"
     gene            complement(3319..>3824)
                     /locus_tag="PLG78_14595"
     CDS             complement(3319..>3824)
                     /locus_tag="PLG78_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNE24400.1"
                     /translation="ISVFGGKLTDCLNIGEEIAELVSECGVKLPYPDAIWYGEPPLEI
                     RQRFFHQAKLMDLDSMTAVDSSEPLSARLWRRYGTSAFALLEDIRDNPQNAEVLIEGT
                     EYIRCEIHMAARREMVTKLDDFLRRRSKIALIEKKETIARAPGIREACKILFGEDADA
                     RYAEYFG"
BASE COUNT          934 a          994 c          944 g          952 t
ORIGIN      
        1 gcattgtttc catcctggct tgtgatctgg aaactggcac tgtaggatcc cattttctcg
       61 taaacggcca taaccgatct ggccacgtcc gcgtgagaga ttcttttctc ttcggctgtg
      121 aggccggcaa tgatcaggag ccctagaatg ctcgctgtga gggtgggcag gcgttttttc
      181 atgcgcacag ggagaagtct caaagaaggg cccagggtca agaccttttc ggcgcatatg
      241 cctccacaca gcggaatggg ggcctgaacg cttttctaaa gctgtttttg gcctgtcgca
      301 gcaaatctga agacttgact tctttttggc gagtgcttgt ttctgccggg aaagtcctga
      361 gatgatcacc acagaaggcc ttgcctacct cacaccgaac ctggccgcca tcctactgac
      421 gcttgcattg tgcattcgct gttggaaaag ccaatccctg ccgggccaca aggaactggc
      481 atttgccctc attgctgagc tactctggat gtgcggtcac attggcaaac tcacctcgcc
      541 cactctggaa ggcaaaatca tatgggatga cattgagttt cttggcacat gcgcctggcc
      601 catggctaca tttgcttttg ctttgaaatt ctcaggagct cgactgcctc gaatcctgca
      661 cgccggactc tggatccttc ctctcttcat tgcaggagcg gcgcttacag atccgtggac
      721 tgcctatttg cacccgaatg caagactcgt gaacactacg ccgttcaccg ggttgctgta
      781 tgatttcaaa ttgattctat ccgtcacatt tcttcagata ctgctgcagg taacgttagc
      841 cgccgttctg atcatcgcgc gatctttctg gctgaccaca agccagagag tgcaatccat
      901 gattctttcc gccggcatcc tgcttccggt gatcggcagt ctgctcattt tactcgggat
      961 tcaattcaca gagcatcgcg acacttcgca catcactttt ggcgttggta acgtcctgat
     1021 ctattttggt ttgttcggaa tttcaaagga agcagagcac agattttcca cgttattcga
     1081 caatgcaagt gatgctattt tgatcatgaa cggattaatg atcattgatt gcaacatcaa
     1141 ggcacagact atctttcagg catccaaaga aaaactcgtt ggtacttccg ttattgatgc
     1201 atgcgcacca gtccaggaag agaggacttg cgaacctcta tggcgagcga acgttgaagc
     1261 tgccatgagt ggccaaccgc agttctttgg ctggactttt gttcgcaaaa ccggagaaga
     1321 gttcgatgca gaagtgagtc taacgtgcac acaggtgggg ccagacatgt tgcttcaggc
     1381 ctttgtgagg gatcgctctg agatcagagc ggcggcgctc gaacgcgaaa gcctgattag
     1441 agatctcaga gcaaagaaca tagaactgga acgcttcaac tattccgtgt cgcacgatct
     1501 aaaaagccct ctgattacca tcaaggggtt tgtaggaatg ctggcacagg atgttgaaaa
     1561 acaaaactac gccaacgtac caaatcatat tcacagaatc agttcagccg cggacaaaat
     1621 gtacagcctg ctcgaagatc tactggcact ttcccgtgcg ggcaaagcaa ccagtgcgcc
     1681 ggcactcaat gagatggacg aaattctgga cgaatcgctg ggcgcacttg aagggctgat
     1741 ccagtctcgc agtgcagaaa tcattcgaaa agagagattg ccactcattt tcgcagacgc
     1801 gatgagaatg aaagcggtat ggcagaacct gatcgaaaat tccatcaagt acgcacgtca
     1861 cggagttcca ccgaaaattc aaatcggctg caaagctgat gcgcaaggat atgcatttta
     1921 cgttcaggac aacggacagg gaattgatcc tcgctaccag cttcaggttt ttgggcaatt
     1981 ccacaaactc gatgcatcca cggaaggatc tggcatcggg ctctctctcg tgcaaagaat
     2041 catggaagcg caccagggtc gggcgtgggt agagtcggat ggagttggcc gaggtgccac
     2101 attctggttt catcttccgc gcaccccgaa ggcgagtgac ggatgagcga aacgttatgc
     2161 gacttctgta atgaagtcca aatctacacg ggaacaaacg ccaccaccaa ctatttgccg
     2221 gacgcgctac gatctgtcat gtatagaaag aatctaaaga gcggggaaat cctgcgttgt
     2281 ccggcatgca atcgcgctta cttttttgac gaacgcactc cgggtggaag cgacgacgcg
     2341 atgacgacta cgttctacca ggatttttat ccgttgactg cggaagaatt aagtgcgctg
     2401 gaacgttagt aactctgcgc tccatgagcg ccgcgtcctg cgagagccgt gaactcatgc
     2461 tgcgggaata attcatgcag agcgtttgta atcatctccg ctgtggccag agaagtgtgc
     2521 gaacctactc caatgaaatt cgagccggca tccacaatga accgtttcac tttatcagac
     2581 tcatcgctta cgatcttaat gtgcccgccg tgagtcggct taaatgcgaa aggctctctc
     2641 tgtaagctta cgtttgtttc ctgatgaggc ttggttccga tcgtataaaa aacaggcttg
     2701 tgactctgtt cgcctggaat gaacgcaagg tacgtcatgt gctttccgcc gacgagcgcc
     2761 tcgagtgcat cgcggaagcg ctgatcgcgc accttctccc agtctatttt ttctttgaat
     2821 tttccaatct tgaacttctg ctgaacgggc acaaacagtg cctgtagcct cgcgtcacgg
     2881 ctgagctcca tgatatgctc cggcgaataa acactctcta caaaagagag gatagatttc
     2941 tgaagatcca gatgctcctt aaatagaacc cagcctgtga tgatggcgcg cgccgcctct
     3001 gcaacggagc tggttgaaaa cctgtgtttg tgtttgaaga actcaaagtc cgatccggac
     3061 cgaagcattc ctacttctgt tacaaacgaa aaattaacca gatctactac gtctcctgct
     3121 atgagaccac ctgactcatc gcggcgaaca aaacagccct gctgttcttt gccgtcacct
     3181 accctgcgaa tcacgtcgcc cggggtaaga gagtctgttt cagtgatcct gatgagatag
     3241 tccatgatcc atcctcaaga atttgcgcct gacagtcgcg gcaaatttac tcacccaaaa
     3301 tacccgccgc gtgcaaagtc atccaaaata ttccgcataa cgcgcgtccg catcctctcc
     3361 aaacagaatt ttacatgcct cgcgaattcc aggagcacgt gcaattgttt cttttttctc
     3421 tataagagca atcttcgatc ttcttcgaag gaagtcgtct agctttgtga ccatttcgcg
     3481 tctggccgcc atatgaatct cgcagcgaat gtactcggtt ccttctataa ggacttctgc
     3541 attctgcgga ttgtctcgaa tgtcttcgag gagggcaaac gcacttgtgc cgtatcgtct
     3601 ccagagccgg gcgctcagag gttctgagga atccaccgca gtcatagaat ccagatccat
     3661 gagcttggcc tgatgaaaga aacgctggcg gatttcaaga ggaggctcac cgtaccagat
     3721 ggcgtccgga tagggaagct tgactccaca ttccgaaacc agttcggcga tctcttctcc
     3781 tatgttcagg cagtccgtga gctttccgcc aaagacgctg atat
//
LOCUS       DAOQJL010000381         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14830_length_3817_cov_2.953482, whole genome shotgun sequence.
ACCESSION   DAOQJL010000381 DAOQJL010000000
VERSION     DAOQJL010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14830_length_3817_cov_2.953482"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLG78_14600"
     CDS             complement(<1..753)
                     /locus_tag="PLG78_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HNE24401.1"
                     /translation="MKRTISVLALLLALSALYVSSQTFASNEHPLDSAMAGPVAPPAD
                     PSVVLRRMSLQIRGTIPTVAEIEAVRANPSSLDQMAESFLRDPDFAHYWGVYFASVFR
                     EQTKGRKVKYASFLNYIAQSLHENKPYDVWTREMITATGSPEKNPAVDMYLRDGADPL
                     QVAEYVGRVFYGERVSCARCHDHPHMKFSRRDYYALAAFFSQQFEVRGSFDPTLFDGR
                     AVPESLLENLPEQKRKEMEQKYRDWHRDAWKNM"
     gene            complement(847..1710)
                     /locus_tag="PLG78_14605"
     CDS             complement(847..1710)
                     /locus_tag="PLG78_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24402.1"
                     /translation="MICEKDRVAFVENNTAHAVPLLLSALAEHGYSPEQVEYAIITHV
                     HLDHAGGSGLLMEKCPNATLLAHPRAARHVIDPARLVASAKMVYGEEVFAKVYGEIQP
                     VEESRVRVMQDGETISFGERSLTFLHTRGHANHHFCVYDSGSDGIFTGDTFGIAYPFM
                     QTQGPHLFPSTTPTEFDPVEARLSIDRILATQASKAFLTHFGEFTHLDEGARQLKSGL
                     DEFEAILKEAKERTETGEALTSFVEEKVNAYFKRRAHFLPETQLNLLLELDGSLNAAG
                     IAFAAERSRKA"
     gene            1850..2101
                     /gene="purS"
                     /locus_tag="PLG78_14610"
     CDS             1850..2101
                     /gene="purS"
                     /locus_tag="PLG78_14610"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNE24403.1"
                     /translation="MYLARIAIRLKPSIQDPQGLAIVHALHELGFSSTESVRMGKYAE
                     VKINAPDKAAAEKAAKDMCEKLLVNSVMEVYDLTVEESR"
     gene            2098..2763
                     /gene="purQ"
                     /locus_tag="PLG78_14615"
     CDS             2098..2763
                     /gene="purQ"
                     /locus_tag="PLG78_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNE24404.1"
                     /translation="MKAGVVTFPGSNCDQDMILVLRQFFGATVESLWHADQFQGSFDL
                     IVIPGGFSYGDYLRTGAIARFAPAMKSVAEHAKRGGVTIGICNGFQILCEAGLLPGAL
                     IRNVTLKHICKDVQLRAKVNPFTRGLTPNQIVTLPVSHGDGNYRADDDVIRSLFDHDQ
                     VAFEYVQNVNGAEKNIAGISDKSGRVLGMMPHPERAVDPATGGTDGKAVLESILNVCR
                     AHV"
     gene            2741..>3817
                     /locus_tag="PLG78_14620"
     CDS             2741..>3817
                     /locus_tag="PLG78_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE24405.1"
                     /translation="MSAELTSDVIRLSLVPLNQIPLDWKGNRARILSALEELKSPAPE
                     IVLFPELCITGYGCEDAFYSLDVAERALSSALEVAQKAHAILDTTVVVGLPVRYHERI
                     YNCAAILREGKIQCIIPKQNLAGDGIHYEPRWFTAWRGRPVETELPDHSQVPMGPMVL
                     DQSGIRIAFEICEDCWVPNRPADKYTEASPDLILCPSASHFAFGKENIRRGIAVDSSR
                     SYACVYAMVNLLGNESGRSIYDGCAIVASLGNLLYEGTRFSFKDYTLHTVDVDIAAVR
                     SARSRLYSFKEQNELTEYAMLPLNIKRAKILSATPPGPPDSMSKELEFLHAVSLGLFD
                     YLRKTHSKGYVVSLSGGADSAACAL"
BASE COUNT          966 a          981 c          926 g          944 t
ORIGIN      
        1 catgttcttc caggcatcgc ggtgccagtc tcgatacttc tgttccattt ctttgcgctt
       61 ttgttccggc aagttttcca ggagagattc cggaactgcg cgaccatcga acagagtggg
      121 atcaaaagaa ccgcgcactt cgaactgctg cgaaaagaaa gcggccagtg catagtaatc
      181 tctgcgagaa aacttcatgt gcggatgatc atgacatcgc gcacaggaaa ccctctctcc
      241 ataaaacacg cgtcccacat attccgctac ctgcagaggg tctgctccat ccctcaaata
      301 catgtctacc gcaggattct tctctggaga tccagtggcc gtgatcattt ctctggtcca
      361 tacatcgtat ggtttgtttt cgtgaagaga ctgagctatg taattcaaga agctcgcgta
      421 ttttactttt cgcccttttg tctgttcgcg aaacactgag gcaaagtata ctccccagta
      481 gtgtgcgaaa tcaggatcac gcaaaaatga ttccgccatc tgatcgagcg aagatggatt
      541 ggcgcgcaca gcttcgattt ctgccaccgt aggaatggtc ccacggattt gcagactcat
      601 cctgcgaaga accacggatg gatctgcagg cggagcgacc gggcctgcca tcgccgaatc
      661 gagtggatgt tcattggatg cgaaagtctg cgacgaaacg taaagtgcgc tcagggccag
      721 gaggagtgcc agaacgctga tggttctctt cattgcatct attggacgac tgcatgcgcg
      781 gacgggtcaa gaaaatgaat cagccccctg actaaaattt aaaggaaacg ctcctaaaaa
      841 atgaactcag gccttgcggg acctttctgc tgcaaacgca atccctgctg cgttcaaact
      901 gccgtccagt tccagaagaa gattcaactg tgtttccggc aaaaagtgag ctcgcctttt
      961 gaagtatgca ttcactttct cttctacaaa ggaagtaagt gcctcacctg tctctgttct
     1021 ttcttttgcc tctttcagga ttgcctcaaa ctcatccagt ccgcttttca actggcgcgc
     1081 cccttcatcc agatgagtga attcaccaaa atgagtcaga aacgccttcg atgcctgagt
     1141 ggctagaatt cgatcaatgc tcagtcttgc ttccactgga tcaaattcag taggtgtggt
     1201 gctcgggaat aagtgagggc cctgagtctg catgaaagga tatgcaatcc caaatgtatc
     1261 ccctgtgaaa atcccgtctg agccggaatc ataaacgcaa aaatgatgat ttgcatgacc
     1321 acgtgtgtgt aaaaacgtca gactcctttc cccaaaagaa atggtttccc catcctgcat
     1381 cacgcgaacg cggctttctt ctacgggctg aatctccccg tacactttcg caaatacttc
     1441 ttctccgtag accatctttg cactcgccac caggcgagca ggatcgatga cgtgtcgggc
     1501 tgcacgagga tgtgcgagca aggtagcatt gggacatttt tccatgagca gtccactgcc
     1561 gcctgcatgg tccagatgca catgagtgat gatggcgtat tctacttgct cgggactgta
     1621 accgtgttcc gccagagcag acaaaagcaa gggaacagcg tgcgcagtat tgttttctac
     1681 aaacgcaact ctgtcttttt cgcaaatcat gtatgcgcag gcagtgagtg gatatagata
     1741 ctgacaatca atggttacta cagttcgcac gcgcagttct ctccccgcca gggaagaatc
     1801 ccactctttt tttacttcca ggcgccggcg gtttcgggaa ctttgcattg tgtatctcgc
     1861 cagaatcgcc attcgtttaa agccctctat ccaggatcct cagggactgg ccatcgttca
     1921 tgctctgcat gaacttggat tttcctccac agaaagtgtg agaatgggca agtatgcaga
     1981 agtaaagatc aacgcacctg ataaagctgc ggcagaaaaa gcagcgaaag atatgtgcga
     2041 aaagctactc gtaaattctg taatggaagt atacgatctc actgtagagg aaagcaggtg
     2101 aaagcaggag tcgttacatt ccctgggtca aactgcgacc aggatatgat tctggtcttg
     2161 cgccaattct ttggagcaac cgtagaaagt ctgtggcatg cagatcaatt ccagggatcg
     2221 tttgatttga tcgtaattcc tggcggtttc tcttacggag attatcttcg cacaggggcc
     2281 attgcacgat ttgctcctgc catgaaatcg gtggcggagc atgcaaagcg cggcggagtc
     2341 accattggaa tctgcaacgg ctttcaaatt ctgtgcgaag ccgggctgct ccctggtgct
     2401 ctcattcgca atgtgactct caagcatatc tgtaaagatg tgcagcttcg cgcaaaagta
     2461 aatccattca ctcgcggcct gactccaaac cagatcgtca ctcttccggt atcacacgga
     2521 gatggaaact atcgcgcaga tgatgatgtc attcggtctc tgttcgacca tgatcaggtt
     2581 gcctttgagt acgtgcaaaa cgtaaacgga gcagagaaaa acatagccgg catttcagac
     2641 aaaagcggtc gcgtactcgg gatgatgccg catccggaga gagctgtgga tcccgccacc
     2701 gggggcacgg acggtaaggc agttctggag tccatactca atgtctgcag agctcacgtc
     2761 tgacgttata cgcctttcac tcgtaccact gaaccagatc cccctggact ggaagggaaa
     2821 ccgcgcccgc attctatctg cactcgaaga actcaaatca cccgcacctg aaatcgttct
     2881 tttcccggaa ctctgcatca ccggttacgg atgcgaagac gcgttttatt ctctggatgt
     2941 agcagaaaga gctctgtcct ccgcgctcga agtggcgcaa aaagcccacg ctatcttaga
     3001 tacaactgtg gtagtaggtt tgccagtgcg ataccacgaa aggatctaca attgtgcggc
     3061 gattctgcgc gaaggcaaga ttcaatgcat cattccgaaa caaaacctgg caggagacgg
     3121 aattcattac gagccaagat ggttcaccgc atggagaggt cggcctgtag aaaccgaact
     3181 tccggatcat agccaggttc ccatgggacc aatggtcctc gatcaaagtg gaattcgaat
     3241 cgcgtttgaa atttgcgaag actgctgggt tcccaatcga ccggcagata aatacacgga
     3301 agcctcccct gatctcattc tttgtccttc tgcatctcat ttcgcatttg gaaaagaaaa
     3361 catccgccgc ggaattgcag tcgattcatc aagatcctat gcctgcgttt acgcgatggt
     3421 gaatctgctt ggaaacgaat cagggcgatc catctacgat ggatgtgcga ttgtggcatc
     3481 tcttggaaat ctactctatg agggcacacg attctcgttc aaagactaca ctctgcacac
     3541 agtggacgtg gacattgcag ccgttcgatc tgcaagaagc aggctctact ctttcaaaga
     3601 gcaaaacgag ctcacagaat atgcaatgct tccactgaac ataaaacgtg cgaagatcct
     3661 ttctgctact ccgcctggtc caccagattc catgagcaag gaacttgaat ttctgcacgc
     3721 agtttctctg gggctattcg attatcttag aaagacgcac tcgaaaggct acgtggtctc
     3781 tctgtccggc ggagccgatt ctgcggcctg tgctctt
//
LOCUS       DAOQJL010000382         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14909_length_3804_cov_4.296346, whole genome shotgun sequence.
ACCESSION   DAOQJL010000382 DAOQJL010000000
VERSION     DAOQJL010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14909_length_3804_cov_4.296346"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="PLG78_14625"
     CDS             complement(<1..1980)
                     /locus_tag="PLG78_14625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNE24406.1"
                     /translation="MPSLSTRYEPASIESRWYEVWEESGVFRPESTGKGEPYCIVIPP
                     PNVTGQLHVGHALNHSIQDVLIRHARKKGKRTLWVPGTDHAGIATQAVVEKLLKKEGT
                     DRFQLGREKFVKRVWEWKDKFGNIITKQMRTLGLSVDWERERFTMDDGLSRAVREMFV
                     ELYKKKLIYRDTRMIMWDPVARTVLSDIEVEYEDNYPGELYSFAYKLSQGDGEIVVAT
                     TRPETMLGDTAIAVHPEDPRFKKLIGKTVKHPFLDREIVIIADSILVDMEFGTGAVKV
                     TPAHDMNDYETGKRHNLESITIFDEAGRINSKGGSFVGLDRFEARKQIKAQLKERGLE
                     RGVKEHKMAIGKSQRSGAIVEPMVSTQWFVSTKPLAKKAIEVVEKGKIRFYPENYANL
                     YNSWMKEIRDWCISRQLWWGHRIPAYHCEDCGHITASVDPPKACEKCKKKNLIQDPDV
                     LDTWFSSGLWPFSTLGWPAKTKDLATFYPTSVLVTSYDIIFFWVARMVMLGLFTMKKI
                     PFHHVYIHGLMRDERGIKISKSLGNNIDPADVIANYGLDAYRFFLMATLSEGKDSTYS
                     ETRLKGYQAFANKVWNSSRFVIMNLPEDFAPVDLAAIVFAKPSRDAKKSTKKSPQKQT
                     LELEQEDWWILSRLAETLTAMDKNISEYKLHLATEN"
     gene            complement(2026..2283)
                     /locus_tag="PLG78_14630"
     CDS             complement(2026..2283)
                     /locus_tag="PLG78_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24407.1"
                     /translation="MRALIAIALLSLAGCQKIKSLWSHSESKIDPCDSVSNYYEGDRH
                     FVERDFVTQDRNLDYRKLFEADKIQNSPCDPVHGRIQKTEH"
     gene            complement(2280..2471)
                     /locus_tag="PLG78_14635"
     CDS             complement(2280..2471)
                     /locus_tag="PLG78_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24408.1"
                     /translation="MNLSSVPGVFAYKIQRYPEQPVQPVERTELSLPERSRRFEMPRL
                     YGPDAQAPDSPGHLIDLFA"
     gene            complement(2535..2912)
                     /gene="apaG"
                     /locus_tag="PLG78_14640"
     CDS             complement(2535..2912)
                     /gene="apaG"
                     /locus_tag="PLG78_14640"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNE24409.1"
                     /translation="MAPVQEIQVKAVARYMPERSNPLEPIYFFSYNITITNQGNLSAR
                     LQSRYWHITDATGRVQEVRGPGVVGEFPNLKPGESFNYTSFCPLSTNYGFMQGHYTME
                     REDGSTFDAPIPPFALAVPDSIN"
     gene            3026..>3804
                     /locus_tag="PLG78_14645"
     CDS             3026..>3804
                     /locus_tag="PLG78_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNE24410.1"
                     /translation="MSQYVYFFGEGRTDAPALDKMLLGGKGANLCEMSQLGIPVPPGI
                     VITTDACKRYVRDNRFPTGLLEEVLVRVQQLNSATGKVFGNPDRPLLVSVRSGAPVSM
                     PGMMDTILNLGLNSETLEGMVRSTGNERFVCDSYRRFVQMYGDVVLGMEHHDFEHILN
                     DVKKSVGAASDTDLRAGDLREIIRQYKDLVRRITGNDFPEDVHEQLRGAIEAVFKSWN
                     NDRANIYRRLNHISDDLGTAVTVQSMVFGNMGDTSGTGVAFT"
BASE COUNT          809 a          936 c          994 g         1065 t
ORIGIN      
        1 gttttcagtg gccaggtgga gtttgtattc gcttatgttt ttgtccatcg ctgtgagcgt
       61 ttctgcgagt ctcgatagaa tccaccaatc ttcctgttcc agttcgagtg tttgtttttg
      121 aggagatttc tttgtggatt tttttgcatc tctggatggt ttcgcaaaga caatggcagc
      181 cagatccaca ggcgcgaagt cttctggcag gttcatgatc acgaatcggc ttgaattcca
      241 caccttgttc gcgaaggcct ggtagccttt gagtctggtt tcagaatacg tagagtcttt
      301 cccttccgaa agcgtggcca tcagaaaaaa gcgatacgcg tccagtccat agttagcgat
      361 cacgtctgcc ggatcgatgt tgttcccgag actttttgaa atctttatgc cccgttcatc
      421 tcgcatcagt ccatgaatgt acacatgatg aaacggaatt ttcttcattg tgaagagacc
      481 gagcatcacc atgcgagcaa cccagaagaa gatgatgtca taggatgtga caagcacgga
      541 agtcgggtaa aatgtagcca ggtcttttgt ttttgcgggc catccaagag tggagaaagg
      601 ccagagaccg gaggagaacc atgtgtcgag cacgtccgga tcttggatta gattcttctt
      661 tttacatttt tcgcatgcct ttggcggatc cacagaggca gtgatgtgac cgcagtcttc
      721 gcaatgatat gccgggattc gatggcccca ccagagctgg cgagagatac accagtctct
      781 gatttctttc atccatgaat tgtatagatt tgcatagttt tctggataga aacggatctt
      841 gcccttttcc accacttcaa tcgccttctt tgccagaggc ttagtgctta cgaaccactg
      901 agtggaaacc ataggttcca caatcgctcc cgagcgctgt gacttgccga ttgccatttt
      961 gtgttctttg accccgcgct caagtccgcg ttcttttagt tgcgctttga tttgctttcg
     1021 ggcttcgaaa cgatcaaggc ctacaaacga tccgcctttc gaattgattc ttcctgcctc
     1081 atcgaagatg gtgatggatt ccagattgtg gcgctttcct gtttcgtagt cgttcatgtc
     1141 gtgtgcgggt gttaccttga cggcgcccgt tccgaattcc atatctacga ggatactgtc
     1201 tgcgatgatg actatttcgc gatcgaggaa aggatgcttt actgttttgc caatgagctt
     1261 cttaaagcga gggtcctctg gatgcacagc aatggccgtg tccccgagca tcgtttctgg
     1321 tctcgtggta gccacgacga tttcgccatc tccctggctc agtttgtacg cgaatgaata
     1381 cagttcgccg gggtagttgt cttcgtattc aacttctatg tctgagagaa ccgtgcgggc
     1441 aactggatcc cacatgatca ttcttgtgtc gcgatagatc agtttcttct tgtagagctc
     1501 tacaaacatt tcgcgcactg cgcgcgaaag tccatcgtcc attgtgaacc gctcgcgttc
     1561 ccagtccacg ctcagtccaa gcgttcgcat ttgctttgtg atgatgtttc cgaatttgtc
     1621 tttccattcc cagactcgtt ttacgaattt ttcgcgacca agctggaatc ggtctgtgcc
     1681 ttctttcttc agtaatttct caaccactgc ctgagttgcg ataccagcgt gatccgttcc
     1741 cggtacccac agcgtacgct tccctttttt tcgcgcatga cgaatgagta cgtcctggat
     1801 ggaatgattg agtgcgtgtc ctacgtgcag ctgccccgtc acattgggcg gtggaatgac
     1861 gatgcagtaa ggttcacctt taccggttga ttccgggcga aagacgccag actcttccca
     1921 gacttcgtac cagcggcttt cgatgcttgc aggttcgtat cgcgtgctca ggcttggcat
     1981 gcgccacttt gcgtgttttt gcctgcgtga aaataagaaa tctaatcagt gctcggtctt
     2041 ctggatgcgg ccatgaaccg ggtcgcaagg agaattctgg atcttgtctg cttcaaatag
     2101 tttgcgataa tcgaggtttc tgtcctgagt cacaaaatct ctttctacga agtgcctgtc
     2161 cccttcgtag taatttgaga ccgaatcgca tggatcgatt ttgctttcgc tgtggctcca
     2221 gaggcttttg attttttgac accccgccag cgacagcagg gcgatagcga tgagggctct
     2281 catgcaaaca ggtcaatcag gtgaccgggg ctgtccggtg cctgcgcgtc tggtccatac
     2341 agcctgggca tttcgaatcg tctgctgcgt tcgggcaggg acaattccgt ccgttccacg
     2401 ggttgtactg gctgttccgg gtagcgctgg atcttgtacg caaatacgcc cggaacgctg
     2461 gacagattca tggagcctga ttcgaccctc atgctttatc tatcggcagg aactggtttg
     2521 ggagttagac gagcctagtt gatgctgtcc ggaactgcca gggcgaaggg aggaatcggg
     2581 gcatcaaagg tggaaccgtc ctctctttcc atagtatagt gtccttgcat gaacccataa
     2641 ttggtcgaga gagggcaaaa gctggtataa ttgaaagatt ctcccggttt tagattcggg
     2701 aattccccca ccactcccgg accccgaact tcctgcacac ggccggtcgc atccgtgatg
     2761 tgccagtaac gggattgaag gcgagcgctc agattgccct ggtttgtgat ggttatatta
     2821 taggagaaga agtagatggg ctcgagcggg ttggaccgtt cgggcatgta acgggctact
     2881 gcctttacct gaatttcctg tacgggagcc atcgcccgga attgtctttg accccggccc
     2941 ggcgctcaag cgttttttca cgttggcttg acagttccga ttcgacgccc actcttcagc
     3001 ctcacgaata tcttcgaggg gatttatgtc acaatacgtt tacttttttg gcgagggccg
     3061 tacggatgct ccggctctcg ataagatgct gcttggcggc aagggcgcga atctctgtga
     3121 gatgtctcag ctcggaattc cagttccgcc gggcattgtc attactacgg acgcatgcaa
     3181 acgctatgta cgcgacaacc gtttccccac tggcctcctc gaagaagttc tcgttcgcgt
     3241 tcagcagctg aattctgcga caggaaaagt ttttggaaat ccggatagac cactgcttgt
     3301 gtccgttcgc tccggagctc ctgtgtccat gcctggaatg atggatacaa ttctaaacct
     3361 cggcctgaat tcggagactc tcgaaggaat ggtgcgatct actggcaatg aaagattcgt
     3421 ttgtgattcg tatcgtcgtt tcgtgcaaat gtacggagac gttgtactgg gaatggagca
     3481 tcatgacttt gaacacatcc tcaacgatgt gaaaaaaagc gtgggcgcgg ccagcgatac
     3541 agatcttcgc gcgggagatc tgagagaaat cattagacag tacaaggacc tggttcgccg
     3601 cataacggga aatgatttcc ctgaagacgt gcacgaacag ctgcgtggag ccatcgaggc
     3661 agtattcaaa tcctggaaca atgaccgtgc gaatatttat cgcaggttga atcatatttc
     3721 tgatgatctg ggtaccgcgg ttaccgttca gtccatggtg ttcgggaata tgggagatac
     3781 gtccggcact ggcgttgcat ttac
//
LOCUS       DAOQJL010000383         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_14983_length_3792_cov_4.691999, whole genome shotgun sequence.
ACCESSION   DAOQJL010000383 DAOQJL010000000
VERSION     DAOQJL010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14983_length_3792_cov_4.691999"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLG78_14650"
     CDS             <1..408
                     /locus_tag="PLG78_14650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24411.1"
                     /translation="VAIILDKEKNVISVLDYGHGYVWLKRGSSVHRLSAHIKHAMAVP
                     FLGIHDDLQMPAGHFLVQNNDVIFACSDGLIEQKNDQREDYGVQRLRNLLRRVSPESP
                     RQINETILEDFNMHIAGSRRSDDLCMLCIRVVV"
     gene            437..769
                     /locus_tag="PLG78_14655"
     CDS             437..769
                     /locus_tag="PLG78_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE24412.1"
                     /translation="MEISHRTQKETDIIQLRGELDMFNSPELGSFIRGRIDSGSKTVL
                     VELEDVTYLDSSGLGVLISAATRMMKASGQFFLVRPSHQVRRLLELTRLTAFFRILNS
                     RDEFLRAT"
     gene            848..2449
                     /locus_tag="PLG78_14660"
     CDS             848..2449
                     /locus_tag="PLG78_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HNE24413.1"
                     /translation="MAEELRKPVAEKPLEKPAEKKRVPRHKLKLRNLTLADFEDVREI
                     MNLVYADFGGTWTREEFGEQVNRFPEGQLCIENKGKVVACALSVIVDYSKYGDNHNYN
                     QITGNGKLTTHDPKGGTLYAVDVFVHPQFQGLRLGRRLYDARKELCRKLNLRRIIAGG
                     WIPGYKQQIDKMTPQQYIELVKQKELYDHVLSFQLANDFHVRKVMLNYFPENKGGYNS
                     VATLLEWINIDYVEPGQPQPLFPKKSVVRVGVIQWQMRRVDSVPDLIQNIEFFVDAVS
                     GYKADFVVFPEFFNAALMAPFNKMMPGDAMRQLSTYTEQIRNEMQQMAVSYNVNIISG
                     SMPEYREHQLYNVSYLLRRDGTFDEQYKLHITPDESNYWGLKGGNKIQVFETDIGKVG
                     ILICYDVEFPELARVLAEQGMEILFVPFSTDTKNAYLRVRHCAHARAIENECYVVISG
                     NVGNLPNVENMDIQYAQSGIFTPSDFLFSQDAVAAEAVPNTESTLIVDLNLDLLKQVR
                     TTGSVRNLENRRLDLYKIHWHEFQG"
     gene            2551..3549
                     /locus_tag="PLG78_14665"
     CDS             2551..3549
                     /locus_tag="PLG78_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNE24414.1"
                     /translation="MDLLFPFQEYWWFYAGFTGFVLLLLTLDLGVFHKEAHAVSYREA
                     GIWSAVWIALALAFNALFYFYSSWKFAQIGLADAEGEARRVALEFLSGYLIEKSLSID
                     NIFVFILIFGYFAIPQKYQHRVLFFGIIGALVFRAAFVAIGAALMQFHWVIYLFGGFL
                     ILTGLKMAFAQEKEMDLSDNLAMKLFKKILPATHDLHGSKFLVKINGIRHATPLLLAL
                     IFVEISDIIFAVDSVPAIFAITKEPLIVFTSNVFAILGLRSLFFLLAGSSDKFHLLKY
                     GLAVVLVFVGLKMVWLNDAFGGKFPISWSLGIILGTIALFMLLSVLFPEKKKANSG"
     gene            complement(3546..>3792)
                     /locus_tag="PLG78_14670"
     CDS             complement(3546..>3792)
                     /locus_tag="PLG78_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24415.1"
                     /translation="QTVVKYENDLIQALHSASAPGVFYAETGASEWAVRFIRFYYDRE
                     SGMTMVDRRYMDAQIRLRREGMGPLNVRLATPVLISD"
BASE COUNT          987 a          925 c          943 g          937 t
ORIGIN      
        1 gttgccatca tactggataa agagaaaaac gtcatcagcg ttctggatta cggtcatgga
       61 tatgtatggt tgaaacgcgg ttcaagcgtt catcgcctca gtgcccacat caagcatgcc
      121 atggccgtac cgttccttgg aatccacgat gacctccaga tgcctgcggg ccattttctg
      181 gttcagaaca acgatgtgat cttcgcctgc tcggacggac tcatcgaaca aaagaacgat
      241 cagcgcgaag attacggagt tcagcgccta cgcaatctgc tcaggcgagt ttcacctgaa
      301 tcgccgcggc agatcaatga aacgattctt gaagacttca atatgcacat cgctggatcc
      361 agaagatccg atgatctatg catgctttgc attcgtgtag ttgtttagtt tataaaattt
      421 attattggag aaaaatatgg aaatcagtca cagaacccaa aaagaaacag acatcatcca
      481 gctgcgcgga gaactcgata tgtttaattc gccggagctc gggagtttca ttcgaggaag
      541 aatcgattca ggaagcaaga ccgtccttgt ggaacttgaa gatgtgacct atctggattc
      601 aagcggcctc ggagttctta tcagcgcagc taccagaatg atgaaagcgt cgggccagtt
      661 ctttctcgtg cgcccttccc atcaggtgcg aagattgctg gaattgactc gtctgactgc
      721 ttttttccga atcctgaatt cgagggatga attcctgcga gcgacatgag agaaaagtca
      781 ttgtgcaggc cgtcgcattc cgggctttat ttgcttgctc tgggctcgct cggcgtcaaa
      841 agacatcatg gccgaagaac tgaggaaacc agtcgcggaa aagccgctgg aaaaaccggc
      901 agagaagaaa cgcgtgccgc gtcataaact aaaactgcgg aacctcactc ttgcggactt
      961 cgaagacgta cgcgaaatca tgaatctggt ctatgccgat tttggtggca catggaccag
     1021 agaagaattc ggagagcaag tcaacagatt cccggaaggg caactctgca ttgaaaataa
     1081 agggaaggtc gtcgcttgcg ccctaagcgt gatcgtggac tattcgaaat acggagacaa
     1141 tcacaactac aatcaaatca caggaaacgg gaagctcacc acgcacgatc cgaaaggcgg
     1201 gactctctat gcggtggatg tatttgtgca tccgcagttc cagggattgc ggctgggtcg
     1261 cagactgtac gacgcaagaa aagaactctg cagaaaactt aaccttcgca ggatcattgc
     1321 aggcggctgg attccaggat ataaacagca aatcgacaag atgactccgc aacagtacat
     1381 cgaattagtc aagcagaagg aactctacga tcacgttctc agcttccagc tcgcaaacga
     1441 ttttcacgtg cgaaaggtga tgctgaacta ttttccagaa aacaaaggcg gctacaactc
     1501 tgtggccact ctcctggaat ggatcaacat agactacgtg gaaccagggc aaccacagcc
     1561 tcttttccca aaaaagtctg tagtgcgagt gggagtgatc cagtggcaaa tgaggagagt
     1621 agattccgtg ccggatctca tccagaacat tgagttcttc gtagatgcgg tgagcggtta
     1681 caaagctgat tttgttgtct tccctgaatt tttcaacgcg gcactgatgg cgccattcaa
     1741 taagatgatg cccggcgatg ctatgcggca gctatccaca tacacggaac agatccgaaa
     1801 tgaaatgcaa cagatggcag tgtcgtataa cgtcaatatc atcagcggaa gcatgccgga
     1861 gtatcgcgaa caccagctat acaacgtcag ctatttgctg cgtcgagacg gaactttcga
     1921 cgaacaatac aagctccaca tcacgccgga tgaatccaac tactggggat tgaagggagg
     1981 aaacaagatt caggtgtttg aaactgacat cggaaaagtt ggaatcctca tatgctacga
     2041 tgttgaattt ccggagctgg cacgagttct cgctgaacaa ggaatggaaa tcctgttcgt
     2101 tccgttttca acggacacga agaacgcata cctgagagtc agacattgcg cgcatgcaag
     2161 agccatcgaa aatgagtgct atgtcgtgat ctcaggcaac gtgggcaact tgccaaacgt
     2221 ggaaaacatg gacatccagt atgcgcaatc cggaatcttt actccgtccg atttcctttt
     2281 ttcgcaggac gcggttgcgg cggaggcggt gccaaatact gaatccactc tcatcgtgga
     2341 tctaaatctc gatctactca aacaggtgag gactacgggc tccgtgcgaa atctggaaaa
     2401 caggagactg gatctataca aaatccactg gcacgagttc cagggataga aatcggacaa
     2461 aattttttac tgcaaactga tttttcgtga tcgccgcctg cccggcggcg cattaacttc
     2521 tcgctcaaca ttttttgatc gagggtatgt atggatctcc tgttcccatt ccaggaatac
     2581 tggtggtttt acgccggatt cactggattc gtattgctgc tcctgacgct ggacctgggc
     2641 gtgttccaca aggaagcgca cgctgtctcg tatcgtgaag caggaatctg gagtgcggtg
     2701 tggatcgcac tggcgctagc attcaatgcg ctcttctatt tctattcttc gtggaaattc
     2761 gcgcagattg gactggcgga cgcagaggga gaagcgcgca gagtggcgct ggaattcctc
     2821 agtggctacc tgatcgaaaa gtctctttcc attgacaaca tcttcgtctt catcttgatc
     2881 tttggctatt tcgcaattcc tcagaagtac cagcatcgcg ttctcttttt tggaatcata
     2941 ggagcacttg tgtttcgcgc ggcatttgtg gccatcggcg ctgctctcat gcagtttcac
     3001 tgggtcatct acctgttcgg tggattcttg attcttactg gcctgaagat ggctttcgcg
     3061 caggaaaagg aaatggacct ctccgataac ctggccatga agcttttcaa aaagatcctg
     3121 cctgccaccc atgatctgca tgggtcaaaa ttcctggtca agatcaacgg gattcgtcat
     3181 gcaactccgc ttcttctggc actcatcttt gtagagatct cggatatcat cttcgcagtg
     3241 gatagcgtgc cggccatctt cgccattacg aaggaaccgc tcatcgtatt cacttcgaat
     3301 gtattcgcca ttctgggcct gcgatctctc ttcttcttgc tcgctggatc ttccgacaaa
     3361 ttccatctgc taaagtacgg cctggccgtg gttctagtct tcgtcggatt gaaaatggtg
     3421 tggctcaatg acgcgttcgg cggaaaattc ccgatttcgt ggtctctggg aatcatactg
     3481 ggaaccattg ccctgttcat gcttctttcc gttctgtttc cagaaaagaa aaaagctaac
     3541 agcggttagt cggaaatcaa gacaggagtg gccagccgca cattcagcgg ccccattcct
     3601 tcacgacgaa gtcgaatctg cgcatccata tatctgcgat cgaccatggt cataccggac
     3661 tctcgatcat agtagaaccg aatgaagcgc actgcccatt cagatgctcc cgtttccgcg
     3721 taaaacacac ctggagcaga agctgaatgg agtgcctgaa tcaaatcgtt ttcgtatttc
     3781 acgactgttt gt
//
LOCUS       DAOQJL010000384         3780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15057_length_3780_cov_4.419597, whole genome shotgun sequence.
ACCESSION   DAOQJL010000384 DAOQJL010000000
VERSION     DAOQJL010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15057_length_3780_cov_4.419597"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLG78_14675"
     CDS             <1..514
                     /locus_tag="PLG78_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE24416.1"
                     /translation="ERLQTTSCDIVLLDLTLPGMDGLTCLEEIKQRFPNVRIIALTMH
                     RDREFFQAAIQRQVDGYVIKEEIFERLLSAIRTVHSGSKYYSEDIRKMMVEDYATIQQ
                     SQTSLDLLSKRELEVLKLIAQGLMNKDIAKRLILSVRTVESHRARMMQKLKISNVQGL
                     VKFAMQKALI"
     gene            617..2611
                     /locus_tag="PLG78_14680"
     CDS             617..2611
                     /locus_tag="PLG78_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE24417.1"
                     /translation="MSEKLSAIIHRFYAPVIKSTTIQARLKLVLIIAVVPPLCLAAWI
                     LFNEITDLRATALEQQGLEYANAIAPMQISIMDHRQFAIDAVRGDRRTETKLRKAETE
                     VAEYSKVISKFTDRHGKEMRVFELWSKITKGWDEIMHTYSSEKDVALTFRRHTEIVQL
                     ETDMIELVGCYSGLVIDPDPATNYLIVSFSRTMPLIVNATGVERGIGRRVLITQKLPD
                     SDREDIERSMAEINLLMGRLHQDTEIAYGYDPEFKARLNALITAAEAQRARLNVTIKN
                     HVLVAQDMDPAEYGTLATNTLSAYRDLNTGISTIVSEHLHARTNSLLLKIGILSLSIV
                     FLLVLLISLVVAIVQSITNPISDAVECVQKVEQGDLTTRLDLNQDDELGILARSLNKF
                     LVSIHGVVKEMVATTGTLQGSARDLTDASQMLAAGTEQMSSQSMNIASAATQMDQNMQ
                     SVSGSAEEMSISIAEVAKQSTHAAQIAQQADQSAIGANKIIQQLGEDARKIGKVIDGI
                     VDIADQTNLLALNAAVEAAGAGEAGRGFSVVAAEVKTLARQARQSADTIKQRVSEIQG
                     SAQSAQGAIVDIAAMIAQVNEVNSSIAAAVQEQSIATREIASNVVQSAQATSEVTKNI
                     SSITDVAKDGAQNAVKVANQANTLQGVSNDLQSTVTLFRI"
     gene            2614..2913
                     /locus_tag="PLG78_14685"
     CDS             2614..2913
                     /locus_tag="PLG78_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24418.1"
                     /translation="MNYFSKKGQSHLHLSVDDLQKDREQLKNYLVSIQDHLEPVVLLH
                     LGDAEETTSGLLALAVAIARHLKENDRTVKLIAEQELVDLLRPLGFERMLELKAS"
     gene            2910..>3780
                     /locus_tag="PLG78_14690"
     CDS             2910..>3780
                     /locus_tag="PLG78_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE24419.1"
                     /translation="MRLPEQDAIRAEFVTEARDLLMRAEECVLRLESQFEPGELLELF
                     RLIHTVKGNTSMLGNPEIGKLAHSLETELDNARQTLAPLSLSQTELVLSGIDRLRTLL
                     DSHEGDAGKPVERVAPAAANRSIANQKADNGVSKKIKVASRHLERARSEGKTLSLITV
                     NLARDPAADLRTSLRAFESLWNGTTVLGAMIREDRLSGLDSTDLSVPCSFLILTPGDP
                     TEIAEINSLSPDHLQVIYSAAPVPTNLPAEVHSRVERIDHPKKTEETHLRVSVAVIDR
                     LINLAGEAVGARNE"
BASE COUNT          998 a          928 c          992 g          862 t
ORIGIN      
        1 tgagaggctc cagacaacgt cgtgtgacat tgtgctactg gatctcacgt tgcctggaat
       61 ggatggattg acctgtctcg aggaaatcaa gcaacgattt ccaaatgttc gtatcatcgc
      121 tctgactatg catcgggatc gcgagttttt tcaggcggcg atccaacggc aggtggacgg
      181 atacgtgatc aaagaagaga tctttgaaag gctactttcc gccattcgca cggttcattc
      241 cggttcaaaa tactattcag aagatattcg caagatgatg gtggaggact atgccacgat
      301 ccagcaatca caaacctcgc tggatctact ctccaaacgt gaactggaag tgctcaagct
      361 catcgcacaa ggattgatga acaaagacat cgcgaagaga ttgattctca gtgttcgtac
      421 agtcgagtct catcgcgcgc gaatgatgca aaagctgaaa atttcgaatg tgcagggcct
      481 tgtaaaattc gccatgcaga aagcgctcat ttaaccattg caaggttccg tactcgtact
      541 gaaaaaacaa gcaccactac caattgaacc atcctgacat tcatgttact gtaaatcaga
      601 aacatgaggt tcgcaggtgt ctgagaagtt gtccgccatc atccaccgtt tttatgcgcc
      661 ggtcatcaaa agcacgacga ttcaggcgcg actcaaactg gtgctgatca tcgcagttgt
      721 tcctccactg tgtctcgcgg cctggattct gttcaatgaa atcaccgacc ttcgcgcgac
      781 cgcgctggaa cagcaaggac tagaatacgc caatgcgatt gctcccatgc aaatctccat
      841 catggatcat cgtcaattcg ccatcgatgc agtgcgaggc gatcgtagga cagaaacgaa
      901 attacggaag gctgaaacgg aagttgctga atatagcaaa gtcatttcaa agtttacgga
      961 ccgtcacggc aaggaaatgc gcgtcttcga actatggtct aaaatcacga agggctggga
     1021 cgagatcatg cacacgtaca gttctgagaa agacgttgcc ctcacctttc gcaggcacac
     1081 tgaaatcgtc caacttgaga cagacatgat cgagctggtc gggtgttatt caggcctggt
     1141 gatcgatccg gatcctgcca cgaactattt gatcgtttct ttctcacgca ctatgcctct
     1201 cattgtgaac gcaactggag tggaacgagg aattggacgc cgcgttctga tcacgcagaa
     1261 gttgccggat tcagaccgcg aagacataga aagatcgatg gctgaaataa acctgttgat
     1321 gggtcgcttg caccaggata cggagatcgc gtatggttac gatccggagt tcaaggcccg
     1381 gctcaatgcc ctgatcacag ccgcagaagc ccagcgcgca cgtttgaacg tgactatcaa
     1441 gaatcacgtg ctggttgctc aggatatgga tcctgcggaa tatgggacgc ttgcaaccaa
     1501 tacgctatct gcttaccggg acctgaatac agggatttcg acgattgttt ctgagcattt
     1561 gcacgcgaga accaactctc tccttctgaa gattgggata ctaagtcttt ctatagtatt
     1621 ccttcttgtg cttctgattt cgctcgtggt tgcaattgtt cagagtatca ccaatccgat
     1681 ttccgatgca gtggagtgcg ttcagaaagt ggaacaagga gacctgacca cgcgcctgga
     1741 tttgaaccag gatgacgagc tcgggatatt ggccagaagc ctcaataagt tcctggtttc
     1801 aattcacggt gttgtgaaag aaatggtcgc cactactgga accctccagg gttccgcgcg
     1861 cgatttgaca gacgcaagtc agatgcttgc ggcggggacg gaacagatgt cttcgcagtc
     1921 tatgaacatc gcgtctgccg ctacccagat ggatcagaat atgcaatctg tttcaggttc
     1981 ggcggaagag atgtctattt ccatcgcgga agttgccaag cagtccacgc acgcagcgca
     2041 gatcgcacaa caggcggatc aatccgcaat cggcgcaaac aagattatcc agcagctcgg
     2101 tgaagatgcg cgaaagattg gcaaagtgat agatggcatc gtagacatcg cggaccagac
     2161 aaacctgctg gctttgaacg ccgcagtaga agctgctggt gcgggagaag caggtcgtgg
     2221 attctcagtc gtcgcagcag aggtgaaaac tctcgcgcgt caggccaggc agtccgcgga
     2281 cacgatcaag cagagagtat cagagattca gggaagcgct cagagcgcgc agggcgccat
     2341 cgttgatatc gcggccatga tcgcacaggt aaacgaagtg aactcaagta tcgcggcggc
     2401 agtccaggaa caatctatcg cgacgcgcga aattgcaagc aacgtggttc aatcggctca
     2461 ggcaacgagt gaagttacca aaaatatttc gagcattacg gacgtggcaa aagatggagc
     2521 gcaaaatgca gtgaaagtgg ccaatcaggc caacactctg cagggcgttt ccaacgatct
     2581 gcaatcgact gtaacactct tcagaatata gatatgaact acttttcaaa aaagggccaa
     2641 tctcatcttc atttgtccgt agacgatcta cagaaagatc gcgaacaatt aaagaactac
     2701 ctggtttcca ttcaggatca cctggaaccc gttgtcctgt tgcatctggg agatgcagaa
     2761 gaaaccacat ccggtttact ggctctggcg gtagccattg cgcgtcatct taaggaaaac
     2821 gatcgtactg ttaaactcat cgccgaacag gagttagttg atcttttgcg cccgctggga
     2881 tttgaacgta tgcttgagtt gaaggcatca tgagactgcc agaacaagat gcgattcgtg
     2941 ctgaatttgt gacggaggct cgcgaccttc tgatgcgagc ggaagagtgt gtcttgcgcc
     3001 tggaaagtca attcgagccc ggggaactgc tggagttgtt tcgactgatt catactgtaa
     3061 aaggtaacac gagcatgctc ggaaatccgg aaattggaaa gctggcgcat tctctggaaa
     3121 ctgagctgga caatgcacgc cagacgctcg ctccgctcag cctatcgcag acggaactgg
     3181 ttctttctgg cattgatcgt ctccgaactc tactggattc acatgaaggc gatgcaggga
     3241 agccagtgga gcgcgtggct ccggcagcgg ccaatcgcag catcgcaaat caaaaagcag
     3301 acaatggagt ttcgaagaaa atcaaagtgg cctcgaggca tctggagcga gccagatcgg
     3361 aagggaagac tcttagtctt ataacagtaa acctcgcgcg tgatccggcc gcggatcttc
     3421 gcacttcact gcgtgcattt gaatcgctct ggaatggaac gactgtcctg ggagccatga
     3481 tcagagaaga tcgcctatcg ggtctggatt caacggatct atctgttcca tgttcatttc
     3541 tcattctgac tccgggcgat ccaactgaaa tagcggagat caactctctg agcccggatc
     3601 acctacaggt gatctattcg gctgccccgg ttcccaccaa tctacctgcg gaagttcatt
     3661 cccgcgtgga acgtatcgac catccgaaga agacggaaga gacacatctg cgtgtttccg
     3721 tcgctgtcat tgatcgactc atcaatctgg cgggcgaagc tgtgggcgcg cgcaatgagt
//
LOCUS       DAOQJL010000385         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15085_length_3775_cov_3.922312, whole genome shotgun sequence.
ACCESSION   DAOQJL010000385 DAOQJL010000000
VERSION     DAOQJL010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15085_length_3775_cov_3.922312"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1128
                     /locus_tag="PLG78_14695"
     CDS             31..1128
                     /locus_tag="PLG78_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24420.1"
                     /translation="MKKRILALTLIVAGLPSCIKISASPFDTSQGADGLAGFLATLLL
                     SSTSMLYVAVGDQGKSYTSSDGRTWTAQTGAGSTITFNAIAWNGSRFFAAGGNTAPQA
                     VIYSSADGITWTNVFTSGTGTGATTFNDIAFTSGKLIAVGQINLSTALIRTSSDGATW
                     STGSGATANVMRIAGDGTNFMAVQQPGSGTVGAYKSSDGITFTAVNQPFATIGKPSIA
                     GDLIFASSRFIFAGSDDSTSATPPSRSSFTTDLGATTWTADSTNKIFAGNAAGEYARA
                     LAYNGTRLEAVGDNCRVDFTTNLTSLSWSSTALTMSGCSAINWKGMIWDGSKFVAVGS
                     SGKFAASATGASTDWTYTTLGSSNVNAIAVR"
     gene            1433..2140
                     /locus_tag="PLG78_14700"
     CDS             1433..2140
                     /locus_tag="PLG78_14700"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE24421.1"
                     /translation="MHDAATAKTSVSEFIRRSFLFPSFLCGPIVLSGQTWIREKFLRP
                     QDVWLICLGLIKVLILLPFWNAHFETGLLLPFQPDVSGFMNLLKTGIYRYAQLYLDFS
                     GIIDIAAGVSGLLGMRIRHNFNRPWLASSVGDFWRRWHITLGAWMRRHVYIPLGKTRF
                     ALFLVFLLVGAWHGLKLHFILWGALHGLAIITERFASSHWSLPEFLARHTIAIRGLRI
                     VLTQLFVTLSWVLFFWA"
     gene            2145..2267
                     /locus_tag="PLG78_14705"
     CDS             2145..2267
                     /locus_tag="PLG78_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24422.1"
                     /translation="MIPKLRLTLSFAVGLLFGLLLYFLLFQSTSEIHHFLYEAF"
     gene            2267..3280
                     /locus_tag="PLG78_14710"
     CDS             2267..3280
                     /locus_tag="PLG78_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24423.1"
                     /translation="MIAREATARGWLIGFFVLGPATFAIFFLQQMLGFFPAPAHCPHS
                     ITLKVSDAELLADARKISQDCVLIFTSSEGGAVGGGKSISELVHQENTTVVSDTEGYS
                     PFRAIVYLGRMNPAWGRELPYPRMILMINSHYARDHEYGSDLFMQYFPEKESANFYKG
                     QAPFPLSQSVGENRSFFTVLAIYRGWMRRRIGLPLERILAGEKTFAKEADLTTDPKWN
                     VSTSISQKIEPESLRKERTIPNAHGLFESALSHLPRERVKLIVMPLHTVVYGNLGLSP
                     MENERNMTSAFTRSGLPYRIVRELDQPGYWFDTIHFNERGRKALAQILAEESTQMMRT
                     KPR"
     gene            complement(3287..3718)
                     /locus_tag="PLG78_14715"
     CDS             complement(3287..3718)
                     /locus_tag="PLG78_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24424.1"
                     /translation="MRENPLREVNIALVATMVTLALAVGVDTILPRIGLAKLTAVHYT
                     HSRVFKATPAGGEIVYKGADPARLKEDLEILSRRFERGHFEIITLPGSKLSNEVTAMK
                     ARAASYRYSVSTGAEEATLRVDGPQEPLAGYLKYLQANWNQ"
BASE COUNT          859 a          999 c          940 g          977 t
ORIGIN      
        1 attgtcccta cccgccagcg ttgttaaagt atgaagaaac ggattctggc cctcactctc
       61 atcgtcgccg ggctgccctc ctgcatcaag atctcagcca gtccgtttga tacatcgcag
      121 ggggcagacg ggcttgcggg cttcctggca actctgcttc tgagttctac cagtatgctt
      181 tacgtcgcag tgggagatca gggcaaatcc tacacttcgt cggatggccg cacatggact
      241 gctcagacgg gcgccggaag caccatcacc ttcaatgcca tcgcctggaa cgggtccagg
      301 ttcttcgcag ccggcgggaa cactgcgccc caggcagtca tctactcttc ggcggatgga
      361 attacctgga ccaatgtttt cacctctgga acaggaaccg gagccaccac gttcaatgat
      421 atcgcgttta catccggcaa gctcatcgcg gtgggccaga tcaatctaag caccgccttg
      481 attcgcacgt caagcgatgg agctacatgg agtacaggtt caggagccac agcgaacgtt
      541 atgcgcatcg caggagacgg aaccaacttc atggcagtgc aacaaccagg cagtggaacc
      601 gtgggagctt ataagagctc ggacggaatt acttttaccg cggtcaatca gcctttcgcc
      661 accatcggaa aaccttcgat tgcgggagat ttgatcttcg caagcagccg gtttattttc
      721 gcaggctcgg acgactccac aagtgccact ccgccttcta gatcttcctt cacaacggac
      781 ctgggcgcta cgacgtggac tgcggatagc acgaacaaga ttttcgccgg aaatgcggcg
      841 ggcgaatatg caagagcact tgcctacaac gggactcgcc tggaagcagt cggggacaat
      901 tgcagagtgg atttcacaac aaacctgacc tctctctcct ggagttccac tgcactcacc
      961 atgtccggat gcagcgcgat caactggaaa ggaatgatct gggacggaag caagtttgtg
     1021 gcagtgggaa gcagcggtaa atttgcggca tctgcgacgg gtgctagcac ggactggacg
     1081 tatacgaccc ttggttcttc gaacgtaaat gcaatcgcag tgcgctgatg gatttcagcg
     1141 tactccagct cacgttcctt ttcacgggcg ttcttgttct gatcagcgtt ctgtcgggta
     1201 agagcgccgc gtcctcgcgc gcttttcctt tgatcacgct ttctgcgact gcggtttttt
     1261 ttttagttta aacgaaacag tcgcacttgt aattccgctc gccgcgctcg cgttttatgt
     1321 cattccctgg cggaccgctc gcaccatcgc tgcgtttgga ttcctctttt tccctgcttt
     1381 tcttagaggg gagccactgg cgaccgctgg atactcttac attattttga gaatgcatga
     1441 tgcggcgact gcgaaaacca gcgtttcaga attcattcgc cgctcctttc ttttcccgtc
     1501 ttttctatgc ggaccgatcg tactttcagg acaaacgtgg attcgcgaga aattcctgag
     1561 gccgcaagat gtctggctga tctgccttgg actgatcaag gttttgattc tactgccttt
     1621 ctggaatgct cattttgaaa caggacttct cctgcctttc caaccagacg tctctggatt
     1681 catgaacttg ctcaagactg gaatctacag atacgctcag ctctatctgg acttttctgg
     1741 catcatagac atcgcagcag gtgtgagtgg cctacttgga atgcgcatcc gacacaattt
     1801 caatagacca tggcttgcct cctctgtggg cgatttctgg agaagatggc acatcactct
     1861 gggggcatgg atgcgacgac acgtctacat tcctctgggc aaaaccagat tcgcactctt
     1921 tcttgtattc ttgctcgtgg gagcatggca tggtttgaag ttgcatttta ttttgtgggg
     1981 agctctacat gggctggcca tcatcacgga gcgtttcgca tcctctcact ggagccttcc
     2041 tgaattcctg gctcgccata ccatcgcaat acgaggattg agaatcgttt tgactcagct
     2101 attcgtcaca ttgagctggg ttttgttttt ttgggcgtag caatatgatt ccaaaactaa
     2161 gactcactct ttcgttcgca gtcggcctgc tttttgggct tctactctac ttccttctct
     2221 ttcagtctac ttctgaaatt catcattttc tatacgaggc gttttgatga tcgcgcgcga
     2281 ggcgacagcc agaggatggc tcattggttt ttttgttctc gggcccgcca cgttcgcaat
     2341 cttttttctc cagcaaatgc ttggtttttt tccggctccc gcacactgcc cgcattctat
     2401 tacgctcaag gtttcagacg ctgaacttct ggcagatgcg cgcaagatct cgcaggattg
     2461 cgttctcatc ttcacaagct cggaaggagg agccgttgga ggagggaaat ccatttctga
     2521 attggttcat caagaaaaca ccacagtcgt atcagacacg gaaggatatt ctccgtttcg
     2581 cgccatcgtt tatctgggca gaatgaaccc tgcctggggg cgtgaactgc cgtatcccag
     2641 gatgatcttg atgatcaatt cacattacgc tcgggatcac gaatatggat ccgatttgtt
     2701 tatgcagtat tttccggaaa aagaatccgc gaatttctac aaaggacagg ccccctttcc
     2761 actttcgcag tcggtgggag agaatcgcag ttttttcacg gtccttgcga tctacagagg
     2821 ctggatgcgc cggagaattg gtcttcccct ggaacgaatt ctcgcaggtg agaagacatt
     2881 tgcgaaagaa gcggatttga ccacggatcc aaaatggaat gtatccactt ctatctcaca
     2941 aaagatagaa cctgagagcc tgaggaaaga aagaaccatt cccaacgctc acggactatt
     3001 tgaatctgct ttatcccatc ttccgcgcga gcgtgtgaaa ctcatcgtca tgcctctcca
     3061 caccgtggtt tatggaaatc tggggctaag cccaatggaa aacgaacgaa acatgacatc
     3121 tgctttcact cgaagcggcc ttccctacag aatcgtaaga gaactggatc aaccgggata
     3181 ctggtttgat acgattcact tcaacgaacg cggcagaaag gctctggcgc agattcttgc
     3241 ggaagaatct actcaaatga tgcgaacaaa gcccagatga ggcgttttat tgattccagt
     3301 ttgcctggag atacttcaga taccctgcca gaggctcctg cgggccgtct acgcgcagcg
     3361 tagcctcctc tgcgcccgtg gacacagaat agcgataaga agcggcacgc gccttcatag
     3421 cagtgacttc gtttgagagc ttgcttccgg gcaaagtaat gatttcaaaa tgcccgcgct
     3481 caaaccttct ggaaagaatt tcaaggtctt ctttcagtcg cgcaggatcc gcgcctttat
     3541 aaacgatttc tccgccagct ggagtggctt tgaagacgcg cgaatgcgta tagtggaccg
     3601 ccgtgagctt tgccagacca attctgggca aaatcgtatc tacacccact gcaagtgcca
     3661 gagtcaccat tgtggcaacc agagcaatat tcacttccct tagaggattc tcacgcatgg
     3721 attcaaaact cggccaggcg cgcgtgctgt cagtaaattt tcagaggaaa cgtat
//
LOCUS       DAOQJL010000386         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15150_length_3765_cov_3.821024, whole genome shotgun sequence.
ACCESSION   DAOQJL010000386 DAOQJL010000000
VERSION     DAOQJL010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15150_length_3765_cov_3.821024"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLG78_14720"
     CDS             <1..1091
                     /locus_tag="PLG78_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNE24425.1"
                     /translation="YSPFNLSYMALSRMNVTSPDEWISAGNLSPEEREQHSYYLAVPK
                     DPRVDKIRARVKEITKDKNWHFEKIAAILRSYKNIQYEVGFDEDQNLDKISKFLFETR
                     KGDCTEFSESAALMGRLAGIPSRVVVGYIASKDLQTPAHRGGIYHIRRRVPVLQKYKM
                     DELYLVTTSHHHAWVQFYLPRYGWVDFETTSFAIPPQPKMDPNNQDVLIPLIEEVPQV
                     REQKFKFPYHFAGRVLAILTASILAALYLYRFFRLMYLKAVARSMTTRGLDALFRVII
                     IRMAREGYDVRPPFLTHAEYARIYPQLGAFAAVFTALRFREHYDEESKIRDWNVLRAE
                     SADVMKRIRKPGAWAGIRRLLTLNGIWI"
     gene            1088..2341
                     /locus_tag="PLG78_14725"
     CDS             1088..2341
                     /locus_tag="PLG78_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE24426.1"
                     /translation="MTGSGIFRFCCGLVFCASVLFLSACSSQVDWIEYRGKNGSGGTA
                     NALHPPLGLRWKLRLHDETGKEAKAFNPPVVIGDMMYFGSIDGNFYALDTETGYMKWN
                     FKTRGAVNSVPFADDQAVYFGSNDGSVYAVDLKDGKQRWSFPTGNTVQSLVLRYKDNI
                     IFTSDTGATFFLDLNGHEQQRLPNPVWSHHTFQVYEGVVYWAPRGRSFGAYDMATKQF
                     LWSVDVTVPYAVWYSFPAIDSKQIYYSSSYFVGGDDPVKLTFYAHDRLTGEATWQNED
                     SIDMGGTPPTRETVFMRHVQLLDYLAPALYKDLVIYTSGDSVVRAFDTKTGSPAWRKN
                     LPYATSSAVTVAGDRIYFGMRGDEAVEGVTGKKPKLICMSARDGNILWQMDTEGAILS
                     SPVISGKRIMFGTDKNYFYVLEEIF"
     gene            complement(2343..>3765)
                     /locus_tag="PLG78_14730"
     CDS             complement(2343..>3765)
                     /locus_tag="PLG78_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lactate dehydrogenase family protein"
                     /protein_id="HNE24427.1"
                     /translation="NIEVFSALDSASLSTVAARMKQIQLKAGDVICNEGEPGHSMFVL
                     ESGELSVQKRGKDKTLVEIARLRPGEVAGIMSLFGDESRSATLISTGEARLWEISNED
                     FQALVQSTPQIAHQLLKVLSRYLREETRIVAELRTRDVENRLKIAFFDSKPYTEEAFK
                     DKNEDKYALQFFESRLSMETLVMAEGFKIVCAFVNDTLDAPVVKKLAAMGIEMIAMRC
                     AGYNNVDLAACKEHGITVANVPAYSPHAVAEHAVALMLSLNRHITRAHSRVREGNFSL
                     NGLVGFDMYGKTAGVIGAGKIGQCVISILAGFGCKVLVYDKFPREQTIPNVRNASLDE
                     IFTQSDIITLHAPLIPETRHIISRESISKMKRGVMIINTSRGGLLDAHALVDGLVSGQ
                     VGSAGLDVYEEEKDYFFEDYSDMILKDDVLARLTTFHNVIVTGHMAFLTREALLNIAD
                     VTLNNVLEFENGLRMGSMKNGLA"
BASE COUNT          909 a          920 c          942 g          994 t
ORIGIN      
        1 gttattcgcc attcaacctg tcctatatgg ctctttccag gatgaatgtg accagtccgg
       61 atgaatggat atctgccggc aatctgagcc cggaagagcg cgaacagcac tcatactatc
      121 tggctgttcc aaaggatccg cgagtggaca agatccgagc gcgcgtcaaa gagatcacaa
      181 aagacaagaa ctggcatttt gaaaagatcg cggccattct tcgcagttac aaaaacattc
      241 agtatgaagt tggattcgac gaagaccaga atctggataa aatcagcaag tttctgtttg
      301 agacaaggaa gggggactgt acagaattct ccgaatctgc cgctttgatg ggtcgtctgg
      361 ccggaattcc ctccagagta gtggtggggt acattgcatc caaagatttg caaacgcccg
      421 ctcacagagg cggcatttat cacattcggc gccgcgttcc cgttttgcag aaatacaaaa
      481 tggatgagct ataccttgtc accacatctc atcatcatgc atgggttcaa ttctacctac
      541 ccagatacgg atgggtagat ttcgaaacta cgtcttttgc catcccgccg caacccaaaa
      601 tggatccaaa caatcaggat gttctgatcc cgctgattga agaagtgcct caggttagag
      661 aacaaaagtt taagtttcca taccattttg cagggcgagt tctcgcgatt ctcacggcga
      721 gcattcttgc tgctctctat ttgtacagat tctttcggtt gatgtatttg aaggcagtgg
      781 ctcgttctat gaccacaaga ggtctggacg cactctttcg cgtgatcatc attcgcatgg
      841 caagagaagg ctatgatgtt cgcccaccat ttttaactca tgcagagtat gcgcgcatct
      901 acccgcaact gggtgcattt gcggcggtct tcaccgcact tcgttttcgc gagcactatg
      961 atgaagagag taagattcgt gattggaatg tacttcgcgc tgaatctgca gacgtcatga
     1021 aacgaatcag aaagccggga gcctgggccg gcattcgtcg tcttttgact ctcaacggga
     1081 tctggatatg accgggtctg gaatctttcg cttctgctgc ggtctagttt tttgcgcatc
     1141 cgttctgttt ctgtcagcct gttcgagcca ggtagactgg atcgaataca gggggaaaaa
     1201 tggttcagga ggcacggcca acgcacttca tcctccattg ggcctgcgat ggaaactgcg
     1261 attgcatgat gagaccggaa aagaagcaaa agctttcaat cctcccgtgg tgatcggaga
     1321 catgatgtac tttggttcca ttgatgggaa cttctacgcg ctggatacag agacagggta
     1381 catgaagtgg aatttcaaga cgcgcggtgc tgtgaactca gttccttttg ccgatgatca
     1441 ggcagtttat tttggatcca acgacggtag cgtctacgcc gtggacctca aggatggaaa
     1501 gcagcgctgg agttttccaa ccggaaatac agtccagtct ctcgtgcttc gatacaaaga
     1561 taatatcatt tttacaagcg atacaggtgc caccttcttt ctggatttaa acggtcacga
     1621 acagcaaagg ttgcccaatc cagtctggtc ccatcacacg tttcaggtct atgagggtgt
     1681 tgtctactgg gcaccgcgcg gacggagctt tggcgcctat gacatggcca cgaagcaatt
     1741 tttgtggagc gtagatgtaa cggttcctta cgctgtatgg tattcctttc cggcgatcga
     1801 ttcaaagcag atctactatt cgagtagtta ttttgtgggc ggagacgatc ctgtgaagct
     1861 cacgttttac gctcatgatc gcctgacggg agaggcaacc tggcagaatg aagactccat
     1921 tgatatggga ggaactcctc ccacgcgcga aactgtattt atgcggcatg tgcagctgct
     1981 cgactatctt gcgccagctc tctacaagga tctggtgatc tacacaagcg gcgactccgt
     2041 ggttcgcgcg tttgatacga agactggaag tccggcctgg agaaagaatc taccctatgc
     2101 gacaagttct gctgtgactg tagccgggga tagaatctat tttggaatgc gcggcgatga
     2161 ggcggtggaa ggagtcactg gaaaaaaacc aaagcttatc tgtatgtctg ctcgcgacgg
     2221 aaatattctc tggcaaatgg atacagaggg agccattctc agctcccctg taatttcggg
     2281 aaaaagaatc atgtttggaa cggacaagaa ctacttttac gttctagaag agattttcta
     2341 aatcaagcca gtccgttttt catagagccc attcttagac cattttcaaa ttcgagcacg
     2401 ttgttcagcg ttacgtctgc gatgttgaga agcgcttcgc gcgtgagaaa ggccatgtgc
     2461 ccggtcacga tcacattgtg aaaagtggtc agccgcgcca gaacgtcgtc tttaagaatc
     2521 atgtctgagt agtcttcaaa gaaatagtct ttctcctcct cgtatacatc caggcccgcg
     2581 cttccgactt gtccgctcac aagaccatct accagggcat gagcatccag aagtccgccg
     2641 cggcttgtat tgatgatcat gaccccgcgt ttcattttcg aaatggattc ccggctgatg
     2701 atgtggcgcg tttccggaat cagaggcgcg tgtagagtga tgatatccga ctgtgtgaaa
     2761 atttcgtcga ggcttgcgtt tcgcacattc ggtattgtct gttcgcgagg gaatttgtcg
     2821 tatacaagca ccttacaccc aaatccagcg agaatcgaaa tcacgcactg gccaatctta
     2881 cccgcgccaa tcacacctgc tgtttttccg tacatatcaa aaccgaccag gccattgagt
     2941 gaaaagttgc cctcgcgcac gcgactgtgt gcacgcgtga tgtggcggtt gagagaaagc
     3001 atcagagcca cggcgtgctc tgccactgca tgaggggaat aggctggaac attggccaca
     3061 gtgatgccgt gttccttgca cgcagccaga tccacgttgt tgtagcccgc gcatctcatt
     3121 gcaatcattt caattcccat ggctgcgagt ttttttacca cgggtgcgtc tagagtatcg
     3181 tttacaaatg cacatacaat cttgaatcct tccgccatga ccagggtctc catgctgaga
     3241 cgagattcaa agaactgaag tgcatacttg tcttcgtttt tgtccttgaa tgcttcttct
     3301 gtatatggct ttgaatcaaa aaaggcgatc ttcagtctat tttctacatc tctcgtacgc
     3361 agttctgcta cgatgcgcgt ttcttcgcgc agatatctgc tgagcacttt gagaagctgg
     3421 tgcgctattt gcggcgtaga ttgcacgaga gcctggaagt cttcattgct gatttcccag
     3481 agccgtgcct ctcctgtaga aatcagagtg gctgatctac tttcgtcgcc aaatagactc
     3541 atgatccccg caacttcgcc gggtctcaag cgcgcgatct cgacgagcgt tttgtctttg
     3601 ccccttttct gtacgctcag ttcccccgat tccagaacaa acatgctgtg accgggctct
     3661 ccttcattgc agatcacgtc tcccgctttc aattgaatct gtttcatgcg tgcggcaact
     3721 gtggaaagag acgcagaatc aagtgcggaa aagacttcga tattt
//
LOCUS       DAOQJL010000387         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15215_length_3755_cov_4.976486, whole genome shotgun sequence.
ACCESSION   DAOQJL010000387 DAOQJL010000000
VERSION     DAOQJL010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_3755_cov_4.976486"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLG78_14735"
     CDS             <1..595
                     /locus_tag="PLG78_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE24428.1"
                     /translation="EEERQRIAGDLHDSVGQLILAAKLNLVSYKSDPTHFADRFDVAL
                     SIIDQASQELREIYTNLYPRSLHDLGLEATVRWHARNILETHGLKVSLEMKAGTLSHE
                     LQVRIFRITQEVFANIVKHAHARSVKLKLSRNKTRIQLTISDDGKGFSPSAPRSGFGL
                     DSIRRRVESVGGEFSIESSRGHGSKFSVTLPQGEAHE"
     gene            588..1232
                     /locus_tag="PLG78_14740"
     CDS             588..1232
                     /locus_tag="PLG78_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE24429.1"
                     /translation="MSNLRVFITDDHAILREGVRLILARIPGIEVVGESGDGLEALQA
                     IENLKPDLVILDISLPSLSGVELARKLRKYQPETKLLVLSRHDGPEYVEQLVKLGIHG
                     YVLKEEAGSDLIKAVEVIQRGQTYLSPRITSQLVSGFDPARSAQDPFHILSNREREIL
                     KLVAEGKSNARIGKELLISPKTVKVHRANIMKKLDVHKVADLVKYAIKAGIVEA"
     gene            1296..2045
                     /locus_tag="PLG78_14745"
     CDS             1296..2045
                     /locus_tag="PLG78_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE24430.1"
                     /translation="MKQLIWDLPTRLFHWTFAGSILAAFALGKFANEHSTLFALHMIF
                     GALAGLMLLWRIFWGFAGSKHSKFKDLLFSPRAIFEYFANVFRGKAQYFAGHNPGGAL
                     SVWIMLGLALVSVATGIGMGFFGEAVEEVHEVVTTLLIFAVAFHITGVIVATIMHNEP
                     YPIAMITGKKTAQSDESISSTRPISAAVFLVIAIAGMTYITKGFDLATRTFHAPGTSF
                     ILKFGENEKEEGGGETENAENSKDSDDDDDD"
     gene            2098..2370
                     /locus_tag="PLG78_14750"
     CDS             2098..2370
                     /locus_tag="PLG78_14750"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNE24431.1"
                     /translation="MILKLAFALSVAITGVDGSLPAQTQEKSFVSLDRVIAEAKRRRP
                     GRLLEVELERTGGQDIYEVEILGDNRVVYKLRFNAVTLEFLGEKEE"
     gene            2367..3038
                     /locus_tag="PLG78_14755"
     CDS             2367..3038
                     /locus_tag="PLG78_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE24432.1"
                     /translation="MRILLVEDDRSILHSVRDKLQADGYQVDTAENGNDADTLGQTAQ
                     YEAILLDLGLPDRGGLALLANWRRRGIQTPVVIITARGSWTEKVEGFNAGADDYIAKP
                     FQYEELLARLRAVLRRANLASQGRRLTRGKIDLLEDSRTVVADGKSSELTAHEFKVLR
                     YLMLHAGIVVSKAKLEEQIYEDDQDHESNVLEVYISRLRKKVGEDRIITRRGQGYLFP
                     REGSE"
     gene            3035..>3755
                     /locus_tag="PLG78_14760"
     CDS             3035..>3755
                     /locus_tag="PLG78_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24433.1"
                     /translation="MKSLRSRLSLAMIAVLIAFFTAQWWFVSAAILDVAESQMRSRLA
                     HDAETIASALTWKNGQPELPPEKTNTEYRRAYSGHYYRVTVGTNEIRSRSLWDFQPAD
                     KSCSSRRSPGPMGQILIVHCLEIRKNGNEVSIWTAEETTDLDSSVSIFQKRYTIITLV
                     AISAILIAQILAVYAGFAPLRSAVVALRKHGSASLHRTLPSEIQPFMEEIKRLVSTLQ
                     SRVDISRKTAANLVHEQKTQLA"
BASE COUNT         1008 a          951 c          947 g          849 t
ORIGIN      
        1 ggaagaagaa agacaacgca ttgccgggga ccttcatgat agcgtcggac aattgatcct
       61 ggccgcaaaa ctcaatctgg tttcctataa aagtgatccc actcacttcg cagatcgatt
      121 cgacgttgct ctgtccatca ttgaccaggc aagccaggag cttcgtgaaa tctacacgaa
      181 cctgtatccc agatcgttgc atgacctcgg gctcgaggca accgttcgat ggcatgctcg
      241 caacattctc gaaactcacg gtctgaaagt gagcctcgaa atgaaagcag gaaccctttc
      301 gcatgaattg caggtgcgta tctttcggat cacgcaggaa gtttttgcaa atattgtcaa
      361 acatgctcac gcgcgaagcg tgaagcttaa actaagtcgc aacaaaaccc gcatccagct
      421 cactatctca gacgatggaa agggtttctc tcccagcgca cccagatctg gatttggtct
      481 ggacagcatt cgaaggagag tggaatctgt gggaggcgag ttttccatcg agtcgagccg
      541 cggacacggt tcgaaattct cagtcactct gccccagggg gaagcacatg agtaatctta
      601 gagttttcat cacagatgat catgcgattc ttcgcgaagg cgttagactg atcctcgccc
      661 ggattcctgg aatcgaagtg gtaggggaat ctggcgacgg cctcgaggct cttcaggcaa
      721 ttgaaaacct taaacctgat ctggtgatac ttgatatttc cctaccttct ctttccggag
      781 tagaactcgc gcgcaagctc agaaaatacc agcctgaaac aaagctcctt gttctatcca
      841 gacacgacgg accggaatat gtagaacagc tcgtgaagct gggcattcat ggctatgttt
      901 taaaagaaga agcaggcagt gatctgatca aagcggtgga agtcatccag agaggtcaga
      961 catatttgag ccctcgcatt acaagccagc ttgtttccgg attcgatccc gccaggtctg
     1021 cgcaggatcc ttttcacatt ctctcgaata gagaacgcga aattctgaag ctggtcgcgg
     1081 aaggaaaatc caacgctcgc attggaaagg aactgctgat ttcccccaaa actgtcaaag
     1141 ttcatcgcgc caatatcatg aagaaactgg atgttcacaa ggtcgcagat ctagtgaaat
     1201 acgccatcaa agctggtatt gtagaggcct gagcccgtaa gatttttttt tcgcacattc
     1261 agcctgcgtt caggttcaca gactatcctg aacctatgaa acaactgatc tgggatttac
     1321 ccacacgcct ctttcactgg acatttgcag gttccattct ggccgctttc gcgctgggaa
     1381 aatttgcaaa tgaacactcc accctcttcg cattgcacat gatcttcggg gctctcgcag
     1441 gactcatgct tctgtggaga atcttctggg gatttgcagg cagcaaacat agcaaattca
     1501 aggatttgct cttctccccc cgcgccatct tcgagtattt cgcaaacgtc ttcaggggta
     1561 aggcgcagta tttcgcaggc cacaatccag gcggtgcact gtctgtctgg atcatgcttg
     1621 gtctggctct ggtttccgtc gcgaccggaa ttggcatggg attttttgga gaagcagtcg
     1681 aagaagtaca cgaagtagtc acaacccttc tcatcttcgc agtggccttc catatcaccg
     1741 gcgtaatcgt agccacgatc atgcacaatg agccgtatcc gattgccatg attacgggca
     1801 agaagactgc tcaatcagat gaatccatct caagcactcg tcccatctcc gccgcggtgt
     1861 ttctagtgat cgcgattgca ggcatgacct acatcacaaa gggcttcgat cttgcgacac
     1921 ggacttttca cgctccgggt acttccttca ttctgaagtt cggcgagaac gaaaaggagg
     1981 aaggcggcgg agaaactgaa aatgcagaga actccaaaga ctcagatgat gacgacgacg
     2041 attagaaaag gcgatgcgcg aaatgtcagc ttgcgaaata ggactgtgag aagcagaatg
     2101 attctcaaac tggcattcgc gctttcagtc gccatcacag gagtagatgg ctccttgcca
     2161 gcacaaacgc aagagaagtc attcgtgtct ctggaccggg tgatcgcaga ggccaaacga
     2221 cgcaggccag gtcgactcct ggaagtagaa ttagagcgga caggcggaca agatatatac
     2281 gaagttgaaa tcctggggga caatcgcgta gtgtataaac tcaggttcaa cgcagtcact
     2341 ctggaattcc tgggggaaaa ggaagaatga gaatcttgct cgtagaggac gatcgttcca
     2401 ttctgcattc tgtcagagac aaactgcagg cagacggcta ccaggtggat acagccgaaa
     2461 atggaaacga tgcggacact ctcgggcaaa ccgctcagta cgaagcgatc ctcctggatc
     2521 tgggattacc ggaccgggga gggctcgccc ttcttgcaaa ctggagaagg agaggaattc
     2581 aaactccagt cgtaatcatt accgcgcgcg gctcctggac ggagaaagtg gagggtttca
     2641 atgcaggcgc agacgactac atagccaagc cctttcagta cgaagaactt cttgctcgcc
     2701 ttcgcgctgt tttgcgaaga gcgaaccttg cttcacaggg acgcaggctg acacgtggaa
     2761 aaatagatct tttagaggac tcacgaactg tcgtcgcaga cggaaaatct tctgaactca
     2821 ccgctcacga attcaaagtg ctgcgatatc tgatgcttca cgccggaatc gttgtatcca
     2881 aggcaaagct cgaagagcag atctacgaag acgatcagga tcacgaaagc aatgttcttg
     2941 aagtttatat cagtcgactg cgaaaaaaag taggagaaga ccgcatcatt accaggcgag
     3001 gccagggata cctctttccc agagagggtt cagagtgaaa tcgcttcgct ccagactatc
     3061 actggccatg atcgcagtgc tgattgcgtt cttcacggct cagtggtggt ttgtatccgc
     3121 tgcgattcta gacgtcgcag aaagccagat gcgttcgaga ctcgctcacg acgccgaaac
     3181 gatcgcctct gcgcttacct ggaagaatgg tcagccggaa ttgccgccag aaaaaactaa
     3241 tacagaatac agacgcgctt actccggcca ttactacaga gtcactgtcg gaacgaatga
     3301 aattcgctca cgctcactct gggatttcca gcccgcagac aagagttgca gttctcgcag
     3361 aagtcctgga cccatggggc agatcctgat cgttcattgt ctggaaatta gaaaaaatgg
     3421 aaacgaagta agtatctgga ctgcagaaga aacaacagac ctggattcgt ctgttagcat
     3481 ttttcagaaa cgttatacga tcatcacgct cgtcgccatc agtgccattc tgatcgcaca
     3541 gatactggca gtatacgcag gattcgctcc gcttcgatcc gctgtcgtgg ccctgcgaaa
     3601 gcatggcagt gcatccctcc ataggactct accatccgaa atccaaccct tcatggaaga
     3661 aatcaaacgg ctggtgagca cactgcaatc gcgcgtggat atttcgcgaa aaacggcggc
     3721 caatctggtt cacgaacaga agacgcaact cgcgg
//
LOCUS       DAOQJL010000388         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15274_length_3743_cov_3.894794, whole genome shotgun sequence.
ACCESSION   DAOQJL010000388 DAOQJL010000000
VERSION     DAOQJL010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15274_length_3743_cov_3.894794"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1513)
                     /locus_tag="PLG78_14765"
     CDS             complement(386..1513)
                     /locus_tag="PLG78_14765"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24434.1"
                     /translation="MAARIFTMIVLCGCILQCGSTVACGDSDQKCSPLLSVLAYQRRT
                     VYNLYVLHYDSNDIGQYRVGDDGIPVYKKSYSGLPAGPGGITSDRTGRTVYVSYDTSA
                     AIQVYRIDDANGDLRLIQTVTGSGLSNSREAATVDVSNRFLYQVNGNGTISVFSIDLW
                     TGMLTNTAISNTTGGGGARFGAIHPTNNYFYSGNVASNTVSFHTRASDGSLSLQGAIT
                     TAGTNGTSQIAIDSTGQYLYAPTFNAGENIYWFPIQANGSLGAVSSISCGNFCYQVWI
                     HPGNRFVFAGGTAGHFMYSRNTSTGALSGPVSTQGASFRSAAGHPSGNFLYTASTGNP
                     VSLRTLSVDSTSGAMTVIDTKSTTGNGTSACGIGLIGSIVF"
     gene            complement(1649..>3743)
                     /locus_tag="PLG78_14770"
     CDS             complement(1649..>3743)
                     /locus_tag="PLG78_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24435.1"
                     /translation="DLQSGAHYSEKQILPAFLAKKTQPKDSHGGVVLTNATVGCFPGF
                     PPIRIDLEDPGNRVPADASAFEKLLKVIVPDVETALGAFQERNSDVFAPDAMPIAMRV
                     SAIFVRGRMQIVDAKGSAIFLPAHEGIENALAFAIRGTTLHALFGDIILDGALPTLIP
                     MAALVKSADMWELKPITSAFRTGSNTVDWNETARFAGVSASAVSVGEIRDELAQNLFT
                     GLATLDGRAADSLVARLKEIGLEKQANLLENISRRKTPEEKLDDTVKLFHVLGIALAR
                     LSGAVTVDKKQLVSVPTFESVFVQKPPSLLPPRDVAKAQAKGTMNRYESALHLAHFYE
                     SMPAEELMANVYPAWADGTATPYVARALAKNAKEAVAICERLLQKRWGARMVRLTALH
                     VLAAVGTKEAEKLLKSASEVGRYSGLPETALAAIAGNLARQVQVTREGKGKSFLDPRE
                     AEKIAELTNRMINARDRNHRMSGMTELAQSGVTSAIPYLRTAFYDEASGEAKTHAAFC
                     LACLGDFESAPTFLEMLRKRSENPDDARTAARALGTLGDIRGIHELLNAWEESWSPGI
                     VAEALKAVGPAAFEFLSERVENNPELLKRKAALSVLENWGSELTPAFQARMELTRGAQ
                     DAEKRMYVLGKLAEANEELTGAWLKEIEEQKLESSEIKRQKKRLMDEKPATPTRTTEP
                     ERLSIFSRIAKGLGK"
BASE COUNT          874 a          994 c          923 g          952 t
ORIGIN      
        1 ttatgctgat ggtctgcgca ctggaataat atgccgcggc agggttgaat gcaggagtgg
       61 cgaccctcca tcctatcgcg aggcttgctg cgagcgcttc cgtattctgt gaggtatcaa
      121 agacactcgg ctcaagccgc atgcaagaag ccgcagtaca tacaagcaag actgcacttg
      181 ttgaagcgaa aactttagaa caatgagaag taacgccttt catcaagatt ccccggaggt
      241 ccacttgttt atgttctgca tgcacagcca gagattaaag aacgagaaca ccccctgaac
      301 agggggtttg tatcatcgtc tatgcgaagc gtcaagagaa ggaagatcgc ctaacgtgcg
      361 cggcgcagat attttagggt tgctctcaaa aaacaatcga accaatcaat ccgatgccgc
      421 atgcagaggt cccattgccg gttgtgctct tcgtatcgat gacagtcata gcgcctgaag
      481 tcgagtcgac ggaaagagtg cgcagcgaaa caggatttcc agtgctcgcc gtataaagaa
      541 aatttccgga aggatggcct gcggcggatc gaaacgatgc gccctgagta gacaccggac
      601 cgctcagcgc cccagtcgat gtgtttctgc tatacatgaa gtgtccggct gttcctcctg
      661 cgaatacgaa gcgattgcct ggatggatcc acacctgata gcaaaaattt ccgcaggaga
      721 tggaggacac cgcgccgagt gatccgtttg cctggatggg gaaccagtat atgttttcgc
      781 ctgcgttgaa agtgggtgcg tagagatatt gaccggtgga atcgattgcg atctgagaag
      841 ttccgtttgt gcccgccgtg gtgatcgctc cctggagcga gagggatcca tcggaagctc
      901 gcgtgtgaaa agacacagtg ttcgacgcta cgtttccaga gtagaaatag ttattcgttg
      961 gatgaatcgc tccaaatctt gccccgcctc cgcctgtagt gttcgagatc gcagtgttcg
     1021 tcagcattcc agtccacaga tctatagaga aaacagatat ggttccattt ccattgactt
     1081 gatacaaaaa acgattcgat acatctacgg tggccgcttc gcgcgaattg gaaaggcctg
     1141 atccagtgac tgtttgtatc aatcgtaggt cgccgtttgc atcgtctatc ctgtatacct
     1201 gaatggctgc ggacgtatca tacgacacgt acacggtccg tcctgttcta tctgatgtga
     1261 tgccacctgg ccctgcaggt aatccggagt aggatttttt ataaacagga attccatcgt
     1321 ctccgactct gtactggcca atgtcgttgc tgtcgtaatg cagaacgtac agattgtaaa
     1381 ctgtgcgcct ctggtaggca agcacgctca aaagaggcga acacttctga tcggaatcgc
     1441 cgcaggctac tgtggaaccg cactgaagga tgcagccgca caaaacaatc atggtaaaga
     1501 ttcgggccgc cattcacctc aatgaatgat ttcgaaaatt cgcgcgcgca agcttttaag
     1561 ttcgcttctc ttttgctttt tcgcaaaaca ctccgtaatt gccgcagaaa atgagccgga
     1621 tctacgtagg aaccgcaaag ccgggtatct attttcccag tccttttgcg atgcgcgaaa
     1681 aaattgataa cctctctggt tcagtcgttc tggtaggcgt cgcaggtttt tcatccatca
     1741 atcgtttctt ctgccgcttg atttcgcttg attccaattt ctgttcctcg atttccttta
     1801 gccacgcgcc cgtgagttct tcattcgctt ccgcgagttt gcccagaaca tacatccttt
     1861 tctctgcatc ttgagcacct ctggttaatt ccatgcgagc ttgaaacgca ggcgtcagct
     1921 ccgatcccca attctcaagc acgctgagcg ctgcttttcg cttcagtaat tccggattat
     1981 tttccacgcg ctctgaaagg aattcgaatg cagccgggcc aactgctttg agtgcttctg
     2041 ccacaatacc tggtgaccac gattcctccc acgcattgag tagttcgtga atccctctaa
     2101 tatctcccag agtgcccagg gctcgtgccg cagtgcgcgc atcgtccgga ttttctgacc
     2161 gcttcctgag catttcaagg aacgtgggcg cggactcaaa atcgccgagg caagcaagac
     2221 agaacgctgc atgtgtcttc gcttcgccac ttgcctcatc atagaaagcg gtcctcagat
     2281 atggaatggc cgaagtcacg ccgctctgcg cgagctcagt catgcctgac attctgtgat
     2341 ttcgatctct ggcgttgatc atcctgtttg taagctctgc aattttttct gcttctcgtg
     2401 gatccagaaa ggacttgcct tttccctctc gtgtcacctg gacctggcgc gccaggttac
     2461 cggcgatcgc cgcaagtgca gtttcaggaa gtccagaata gcgtccaact tcgcttgcac
     2521 tcttcaaaag cttctctgct tccttcgtgc caactgctgc cagtacatgc aaagcagtca
     2581 ggcgaaccat ccgcgctccc catctctttt gcaaaagtct ttcacagatg gccaccgctt
     2641 ccttcgcgtt cttcgcaaga gctctcgcaa catagggtgt tgcagtcccg tctgcccaag
     2701 ccggatatac attcgccatc agttcttctg ctggcatgga ttcataaaaa tgagccagat
     2761 gtaaggccga ctcgtatcga ttcattgtgc ccttcgcctg cgctttcgca acgtcgcgtg
     2821 gcggcagaag cgaaggtggt ttttgcacga acacactttc aaatgtggga actgaaacga
     2881 gttgcttctt atcaacagtg acggcaccgg acagcctggc cagggcaatg ccgagcacat
     2941 gaaacagctt cacagtatca tccagtttct cctccggggt tttgcgccgc gaaatattct
     3001 ccaataaatt tgcctgtttc tcaagaccaa tttccttgag acgcgccacc agtgaatccg
     3061 ccgcgcgccc atcgagagta gcaaggcccg tgaataaatt ttgcgccagt tcgtcccgga
     3121 tttctccaac agaaactgcg gaggctgata cgcctgcgaa gcgagcagtc tcattccagt
     3181 ccaccgtgtt ggatcctgtt ctaaatgccg atgtgatcgg cttgagttcc cacatatccg
     3241 ctgacttaac aagagctgcc attggaatca aagtgggaag cgcaccatct agaatgatat
     3301 caccgaaaag tgcgtggagg gtggttcctc taattgcaaa ggcgagtgca ttctcgattc
     3361 cttcgtgcgc gggtaggaag attgcggagc cttttgcatc cacgatttgc attctacctc
     3421 gaacaaaaat cgcgctgact cgcattgcaa tcggcatcgc atcgggagca aagacgtccg
     3481 agtttctttc ctgaaaggct ccaagcgcgg tttctacatc cggcacaatg actttcagaa
     3541 gtttttcgaa ggcggaagca tctgcaggca ctctgtttcc tggatcttca agatcaatac
     3601 gaatgggtgg aaagccagga aaacaaccaa ctgtcgcatt cgtcaaaacc actccaccgt
     3661 ggctgtcttt tggctgagtt ttttttgcga ggaatgcagg cagaatttgt ttctcggagt
     3721 agtgcgcccc actctgaaga tca
//
LOCUS       DAOQJL010000389         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15290_length_3741_cov_3.415627, whole genome shotgun sequence.
ACCESSION   DAOQJL010000389 DAOQJL010000000
VERSION     DAOQJL010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15290_length_3741_cov_3.415627"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLG78_14775"
     CDS             <1..196
                     /locus_tag="PLG78_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24436.1"
                     /translation="PLELAQRLDFSSTYYFLSRVVNAKMAKMNGSLPDYDSPINKWAL
                     QLPVFGQMGQTILWIWRNPH"
     gene            complement(226..2253)
                     /locus_tag="PLG78_14780"
     CDS             complement(226..2253)
                     /locus_tag="PLG78_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE24437.1"
                     /translation="MKTLAIIQARMGSTRFLNKVLKPVDGVPLIELLLQRLSRARKID
                     RIILATSLDPRNQPLADCVRALGYEVYQGSENDVLDRYFQAALPHKPEDVVRITGDCP
                     LIDPGIVDEVITAFEDQQVDYLTNTLPPTYPDGLDVEVFTFAALKRAHEESTKPIERE
                     HVTPFIRNSGLFKLGNISYKEDLSSERWTVDEPEDFAVVEKIFQHFRPNVHFGWLEVI
                     ELSKTHPEIFQANRHFIRNDGYLMGSGQKLWKRARKIIPGGNMLLSKRAEMFLPDQWP
                     AYFSKAKGCRVWDLDGNEYIDMSVMGIGTNTLGYGDPEVDAAVSAVVSAGNMSTLNCP
                     EEVYLAEKLVELHPWADMVRFARSGGEANAVAIRIARAASGRSKVAFCGYHGWHDWYL
                     AANLGDEANLAGHLLPGLSPNGVPSNLRDTVYPFNYNDYAQLESIVSSQQIGVIMMEV
                     SRNLGPEPGYLEKVRKLATDRGIVLIFDECTSGFRQTLGGLHKMYGVEPDMAMFGKAL
                     GNGYAITACIGRREIMEAAQTSFISSTFWTERIGPAAGLKTLEIMERTRSYEQITATG
                     RSISERWEKLAEKHSLEIDVFGLPALTGFVFKSENHLPYRTLLTQEMLARGFLAGTSA
                     YACTAHTPEIIDAYFNELDPIFATIRECEEGRDVSTLLKGPVVHSGFKRLN"
     gene            complement(2250..3128)
                     /locus_tag="PLG78_14785"
     CDS             complement(2250..3128)
                     /locus_tag="PLG78_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE24438.1"
                     /translation="MMQHLSRIALGTVQFGMAYGVANAGQVRREDASAILDHAWKSGI
                     RALDTAAAYGESETVLGEIGVAEWTLTSKLPPVPEDSEVEGWLRTAAESSLRRLRVPR
                     IHAILLHSPDQLLSSRGESIYRALAKLREEGLVSRIGISAVRAEDAIELFSRYDLNAV
                     QAAMNVLDRRFESSHALRKMKDARAEVQIRSIFLQGLLLMNAQSRPAKFKRFDSVWRR
                     WDEWLKDKNVTALSACLSFALQIPGADRIVLGVDSLDHLKEIIASIPSQKNIDIPANL
                     ASIDAALLDPWRWGEL"
     gene            complement(3125..>3741)
                     /locus_tag="PLG78_14790"
     CDS             complement(3125..>3741)
                     /locus_tag="PLG78_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE24439.1"
                     /translation="ISIFSFHPVKHIASGEGGMITTNNRALYDKVSLLRTHGITRDTA
                     SYQNRDMAGEGAWYYEMQELGFNYRMPDILAALGLSQLRRAEEGLARRRAIARRYDSA
                     FANSSAAPMIPTESELHAYHLYIVRVENRRAVYDRLREKGILAQVHYVPVHLQPFYRR
                     LGFGPGDFPAAEQFYSSAISLPMFPGLSDSDVDRVIASVLESVE"
BASE COUNT          914 a         1009 c          937 g          881 t
ORIGIN      
        1 gccattagag ttggcgcagc gacttgattt ttcttccacc tactatttct tgagtcgcgt
       61 agtgaatgcc aagatggcaa aaatgaatgg aagcctcccg gactacgatt cgcctataaa
      121 caaatgggcc ttgcaactcc ccgtgtttgg ccagatgggc cagacgatat tatggatatg
      181 gagaaatccg cactaattgc ccatgtcgcg tgggtagtac atcaactaat tcaaccgctt
      241 gaaaccgctg tgaaccacgg gtcccttcaa gagagtagac acatcgcgcc cctcttcaca
      301 ctctcggatt gttgcgaaga ttggatccag ttcattaaaa tatgcgtcga tgatttcggg
      361 cgtgtgtgcg gtgcaggcat atgcgcttgt tcccgccaga aatccccgcg caagcatttc
      421 ctgcgtcagc agtgtgcgat aagggaggtg attctctgat ttaaaaacaa agccggtcag
      481 cgcaggaagt ccgaatacat caatctccag cgaatgtttt tcggccaatt tttcccaacg
      541 ctctgagata cttctgcccg tcgccgtaat ctgttcatat gacctggtgc gctccatgat
      601 ttccagtgtc ttgagcccgg cagctggtcc gattctttcc gtccagaagg tactgctgat
      661 aaacgaagtc tgcgcggctt ccatgatctc acgccggcca atgcacgctg tgattgcata
      721 tccgttgccg agcgcttttc caaacatggc catgtcaggc tccacgccgt acattttatg
      781 aagaccgcct aacgtttgac gaaatccgga agtacactca tcaaagatca aaacaattcc
      841 acgatccgtt gcaagcttgc gcaccttttc cagatatcca ggttcaggcc cgaggttgcg
      901 cgacacctcc atcatgatca caccgatctg ctgcgaagaa acaatggact cgagctgtgc
      961 gtaatcgttg tagttaaacg gatacacggt gtcacgcaga ttggaaggca ctccattcgg
     1021 agaaaggccc ggcagcagat gccccgccag attggcttcg tcccccagat tcgctgcgag
     1081 gtaccagtca tgccatccat ggtaaccgca aaacgccact ttcgatcttc cggaagccgc
     1141 gcgagcgatt cgaattgcaa ctgcgttcgc ctctccccct gaacgcgcaa agcgcaccat
     1201 gtcagcccat ggatgaagtt ctacaagttt ctctgccaga taaacttctt cggggcaatt
     1261 gagggtgctc atatttccgg ccgatacaac cgcactgacc gctgcatcaa cttcgggatc
     1321 gccgtatcca agggtgttcg ttccaattcc catcaccgac atgtctatgt attcgttgcc
     1381 gtccagatcc cagacgcggc agcccttcgc cttgctgaag taggcgggcc attgatctgg
     1441 aagaaacatc tccgctcttt ttgagagcag catgttgccg ccaggaatga ttttacgagc
     1501 acgcttccac agcttctgtc cggatcccat aagataaccg tcgttacgaa taaaatgtcg
     1561 atttgcttga aagatctcag gatgagtctt tgagagttca attacttcaa gccagccaaa
     1621 atgcacattc gggcgaaagt gctggaagat cttctctaca actgcaaaat cttccggctc
     1681 atccacggtc cagcgttccg acgaaagatc ttctttgtag gaaatattgc ccagcttgaa
     1741 aagtccggag tttcgaatga atggagtcac atgttcgcgt tcaattggtt tcgtgctttc
     1801 ctcgtgcgcg cgtttgagcg cggcaaatgt gaaaacttca acatccagac catcaggata
     1861 cgtcggcggc agagtattgg tgaggtagtc aacttgctgg tcctcaaaag cggtgatcac
     1921 ttcgtccaca attcccgggt cgatgagagg gcagtctccc gttatgcgca ctacatcctc
     1981 cggcttatgc gggagtgcgg cctggaaata tcggtccagt acatcatttt cgcttccctg
     2041 atagacttcg tatccgagcg cacgaacgca atctgccagt ggttgattgc gcggatctag
     2101 ggaagtagca agaataatgc ggtcgatctt tcgcgcgcgc gaaagccgct gcaaaaggag
     2161 ctcaatgaga ggcacgccgt ctacaggttt caataccttg ttcagaaacc gggttgaacc
     2221 catgcgggcc tgaatgattg cgagtgtttt caaagttccc cccatctcca gggatcaagc
     2281 agtgccgcat ctatagatgc aagatttgcc ggaatgtcta tatttttttg cgaaggaatg
     2341 cttgcgatga tttcttttag atgatccagg ctgtctacgc cgagtacaat tctgtctgct
     2401 cctggaatct ggagcgcgaa ggaaagacag gcagaaagcg cggtaacatt cttatccttg
     2461 agccattcat cccagcgcct ccagacggag tcaaagcgct tgaatttcgc cggacgcgac
     2521 tgcgcattca tgagaaggag cccctggaga aaaatggacc tgatctgtac ttccgcccga
     2581 gcatctttca tttttcgcag cgcgtggctc gattcaaaac gacgatccag cacgttcatc
     2641 gcggcctgaa cagcattgag atcatagcgg gaaaaaagtt caatggcatc ctccgcgcga
     2701 acggcagaga tgccaatgcg agagacaagt ccttcttctc gcagcttcgc cagcgcgcga
     2761 tagatgcttt caccgcgcga agaaagaagc tgatccgggc tgtgcaacag aatggcatgg
     2821 atgcgaggta cgcgaagcct gcgaagagat gattccgccg ccgtccgcag ccacccttcc
     2881 acttcactgt cttcgggaac aggcgggagc ttggaagtca aagtccactc tgcaactccg
     2941 atttcgccca gtactgtttc gctttcgccg tatgcggcag ccgtgtcgag tgcgcgaatc
     3001 ccgcttttcc aggcatgatc tagaatcgcg gatgcatcct cgcgccgtac ctggcctgca
     3061 tttgccacgc cataagccat tccaaactgc accgtgccaa gcgcgatgcg gctcaaatgt
     3121 tgcatcattc tacagactca agaacagagg cgattacgcg gtccacgtca gaatcagaaa
     3181 gccctggaaa cataggaaga ctgatcgcgg aagaatagaa ttgttcagct gccggaaaat
     3241 ctccgggccc aaatccgaga cgtctataga aaggctgcaa gtgcaccggc acatagtgta
     3301 cctgtgcaag aatacccttt tcgcgcagac gatcgtaaac ggcccggcga ttctctaccc
     3361 gaacaatgta aaggtgatac gcatgcaatt cagactcagt cggaatcatg ggtgcagcag
     3421 aagaatttgc gaaagcactg tcatagcgcc tggcaatcgc acgtcttcgc gcaaggcctt
     3481 cttccgcacg gcgaagctgc gaaagaccga gtgccgcaag aatgtcgggc atgcgataat
     3541 tgaatccaag ttcctgcatc tcgtaatacc aggcaccctc tcccgccata tctctgttct
     3601 ggtaagatgc agtgtcacgc gtgatcccgt gcgtgcgaag aagagatact ttgtcataga
     3661 gcgcgcggtt gttggtagtg atcatcccac cttcgcctga ggcgatgtgt tttaccggat
     3721 gaaaagaaaa gatagaaata t
//
LOCUS       DAOQJL010000390         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15297_length_3740_cov_4.862687, whole genome shotgun sequence.
ACCESSION   DAOQJL010000390 DAOQJL010000000
VERSION     DAOQJL010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15297_length_3740_cov_4.862687"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLG78_14795"
     CDS             <1..468
                     /locus_tag="PLG78_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24440.1"
                     /translation="VCARLAKNGLSVSVTDMEGKRPPPSYTIESLREIQSQNPSLRIS
                     LLVGSDSLADFASWKDPDAIAREFDLMVAYRITDKEAESSIRKVESEFKARITALPGE
                     IPGCASRDIRRWIRSEASGLVKEGGEESLLKDCMARDVLDYIRAQGLYLNVSN"
     gene            455..1573
                     /locus_tag="PLG78_14800"
     CDS             455..1573
                     /locus_tag="PLG78_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE24441.1"
                     /translation="MSRISRILLVASAIFVIAASFLFVRRFFSNQLEKRILDRDVVPI
                     RVIVRDQDRIETLAQMILFPEQKWVLFYFVNTDARQPEGSKSIADQTAMFADSFDKYS
                     GLKSIYSVELSRDSFARLIDLGEGLPMYLETAQIFPNSNFQYPAGRMRFSGQQAAEYA
                     LSRRMQQGQEQLSGLERMRRMETILLNVAWNSVQFADKIHSKDLRQFALGLIESDLTK
                     PELASLFNFLSDANISILEVPLEQGADRALYVKDKRARSVYSEFFDNLRSGRMKKDVF
                     SLEVLNGTEVGGLARRVKQFLQDRGILVLNADNYPIKPIPKSVILVRAGKTALGKHVM
                     EMTSLPREGVFFDRSTQDVDCTILLGTDFEIKKLQVFE"
     gene            1576..2025
                     /gene="rsfS"
                     /locus_tag="PLG78_14805"
     CDS             1576..2025
                     /gene="rsfS"
                     /locus_tag="PLG78_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNE24442.1"
                     /translation="MAKKAVKASAPRKSPTKPGPSEKLVKEVTELLQEKKASRIVWMN
                     LTAVNPYFDFFLLASAGSRVQLKSLVKDIEKKFPSLERRNSSISADDMDSGWLVLDFG
                     DVVVHLFLEEQRRYYNLERLWGDAVITEIQDESTRESRLLSEQSAES"
     gene            complement(1958..2341)
                     /locus_tag="PLG78_14810"
     CDS             complement(1958..2341)
                     /locus_tag="PLG78_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24443.1"
                     /translation="MMQKSLRITFILALVCGTLLLLPAFAMLVAAGLALTGKMQTQAN
                     TQIVPALIFSLVLLSLSLVPIAAFFLRKTERGMKLLKIAGWIFLIPAAAFPLALGLPI
                     GSAIMTLLIALTGVWTLVSTRPESQ"
     gene            complement(2338..3075)
                     /locus_tag="PLG78_14815"
     CDS             complement(2338..3075)
                     /locus_tag="PLG78_14815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE24444.1"
                     /translation="MKTFVLAFGNFFFRWRDTAFSLVFLTAFLTAGIAVLNPDGFQWD
                     VRTSIIGLIIALAGQFVRGITIGYAYIKRGGLNKKIYAETLQRRGVFAHTRNPMYLGN
                     LLLVTGALVSINNIAFYALALPLFYFIYYAIIVAEEEFLGKKFGEDYVKYTKEVNRLL
                     PGDMGRWSETVKEMEFSFKRLIRKEHGSTFVIFAGTCLYNITKFHLRYGIPLEAMTVL
                     WGIVGALVVLQIIAEVLKRTGQLAEAE"
     gene            complement(3102..>3740)
                     /locus_tag="PLG78_14820"
     CDS             complement(3102..>3740)
                     /locus_tag="PLG78_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE24445.1"
                     /translation="FDAHVSKSVPLYAEGHEIIAKVSDYFVQDGSTIYDLGCSTGELL
                     RKIGERHAGKDIRLIGVDRVEGMLEVARDKCKEDPRIQFECAELLEFEMQPADFVFAY
                     YTVQFVRPAFRQDVINRIWKGLQWGGAFLMFEKVRGPDARFQDILTGLYNDYKLEQGY
                     SSEEIIGKARSLKGVLEPFSTAGNIDLLHRAGFKDCMTIMRYLNFEGTLAIK"
BASE COUNT          974 a          861 c          950 g          955 t
ORIGIN      
        1 gtgtgcgcgc gccttgcgaa gaatggtctt tcggtgtccg ttacggatat ggaagggaag
       61 aggccgcctc cgagttatac cattgagagt cttcgcgaaa tccaatcgca gaatcccagc
      121 cttcgcattt cgctcctggt tggatcagac agtctggccg attttgcatc gtggaaggat
      181 ccggacgcaa ttgcgcgcga atttgatttg atggtagcat acaggatcac ggacaaagag
      241 gctgagtctt cgatccggaa agtggaatct gaattcaaag cgcgcatcac agctctgccc
      301 ggagaaattc cagggtgcgc aagcagggat attcgccgct ggattcgatc ggaggcgtct
      361 ggtttggtaa aggaaggagg tgaggagagc ctattaaagg attgcatggc cagagacgtt
      421 ctcgactata tccgcgcaca gggtctttat ttgaatgtct cgaattagca gaattttact
      481 cgttgcctcc gcgatctttg tgatcgctgc atcctttctt tttgttcgca gattcttctc
      541 caaccagctg gaaaagagaa tcctcgatcg cgatgtagtg cccattcgag tcatcgtgcg
      601 ggatcaggac agaatcgaaa ccctggcgca gatgatcttg tttccggaac aaaagtgggt
      661 gttgttctac tttgtaaata cggatgcccg tcagccagag ggttctaaat ccattgcaga
      721 tcagactgcg atgttcgcag acagctttga taagtactca ggtcttaaat ccatctactc
      781 tgtagaactt tcgagggatt cctttgcccg tttgattgat ctgggggaag ggcttcccat
      841 gtacctggaa accgcacaga tctttcccaa ttctaacttt caatatccag cagggcgaat
      901 gcgcttttca ggccagcagg cggccgagta tgctctttcc agaagaatgc agcagggtca
      961 agagcagctc agcgggctgg aaagaatgcg aaggatggaa acgatccttc tcaatgtggc
     1021 ctggaactcc gttcaatttg cagacaagat ccattccaaa gatctcagac agtttgctct
     1081 tggactcatc gaatctgatc taacaaagcc tgaacttgcc agtctcttta attttctatc
     1141 agacgcaaac ataagcattc tggaagttcc tctggagcag ggtgcagaca gagcattgta
     1201 cgtgaaagat aagcgagcgc gaagcgtgta ttctgagttc ttcgacaatt tgagatctgg
     1261 tagaatgaag aaagatgtct tctccctgga agtgctcaac ggtacagagg tgggcggact
     1321 tgcgcgcaga gtgaaacaat ttctgcagga tcgtggcatt ctggtgctga atgctgacaa
     1381 ctatccaatc aaacccatcc ccaaaagcgt aattcttgtg agagccggca aaacggccct
     1441 gggaaaacat gtaatggaaa tgacgtcgtt gccaagagaa ggtgttttct ttgatagaag
     1501 cactcaggac gtagattgta cgattctact ggggactgat ttcgagatta agaagcttca
     1561 ggtatttgaa taagaatggc aaagaaagca gtcaaagcaa gcgctcccag gaaatctcca
     1621 acaaagcctg gcccctcaga aaaacttgta aaagaagtca cggagctcct ccaggaaaag
     1681 aaggcaagtc ggatcgtctg gatgaatctc accgcggtaa atccgtactt cgattttttt
     1741 ctgctggcgt ccgctggatc gagagtgcag ctcaagtctc ttgtaaaaga catagagaag
     1801 aagtttccgt ctctggaacg tcgcaatagt tccatcagcg cggacgatat ggattcaggc
     1861 tggctggttc tggattttgg ggatgttgta gttcatcttt tcctggaaga gcaacgtaga
     1921 tattacaacc tggaaagact gtggggtgat gcggtcatca ctgagattca ggacgagtcg
     1981 acacgagagt ccagactcct gtcagagcaa tcagcagagt cataatcgca gaaccaattg
     2041 gcagtcccag cgccagcgga aacgcggccg cgggaatgag aaatatccat ccagcaatct
     2101 taagaagctt cattcctctc tctgttttac gcaaaaagaa agcggcaatc ggaaccagag
     2161 agagggacaa tagaacaaga gagaatatca gggccgggac tatctgagtg tttgcctgtg
     2221 tttgcatttt tccggtgagt gcgagcccgg ctgcaacgag cattgcgaat gcgggcaaga
     2281 gcagcaaggt tccgcaaacc agggcgagaa tgaaagtgat acgaagagat ttttgcatca
     2341 ttccgcttct gcaagttggc cagtgcgttt caggacttca gcaatgatct gtaacacaac
     2401 cagtgcgccc acaattcccc acaaaaccgt catcgcctcg agcggaatgc cgtatctcaa
     2461 gtgaaacttt gtaatgttgt agagacatgt acctgcgaaa ataacgaatg tgcttccgtg
     2521 ttccttgcgg atcagtctct taaaagaaaa ttccatttcc ttcacagttt cagaccacct
     2581 gcccatgtct cctggtagga gacgattgac ttcttttgtg tactttacgt aatcctctcc
     2641 gaattttttt cccaggaatt cttcttctgc cacaatgatt gcgtagtaga tgaagtagaa
     2701 cagaggaagt gcaagtgcgt aaaacgcaat gttgttgatg gaaacaagtg cgccggttac
     2761 aagaagtaga ttaccaagat acatcgggtt tcgtgtatgc gcgaagacgc ctcttctctg
     2821 gagagtttcc gcgtagatct tcttgttcag accgccgcgc ttgatgtacg cataaccgat
     2881 cgttatgccc cggacaaact ggcctgccag ggcaatgatc agtccaatga tggaggtgcg
     2941 aacgtcccat tgaaagccgt ccggattcag gactgcgatg cctgcagtca gaaatgctgt
     3001 cagaaatacg agagagaacg cggtgtctct ccagcgaaaa aagaaattac caaatgcaag
     3061 tacgaaggtt ttcattttga ttccgttttg agtattcttg tctatttaat ggcgagtgtg
     3121 ccttcgaaat tcaaatagcg catgattgtc atgcagtctt tgaagccggc tctgtgaagc
     3181 aagtcgatgt tcccggcggt tgagaacggt tcaagtacac ctttgaggct gcgggctttt
     3241 ccaatgattt cctctgagga gtaaccctgt tccaatttgt agtcattgta gagtccggtg
     3301 agtatgtcct ggaagcgcgc atccgggcct ctcacttttt caaacatcag gaatgctccg
     3361 ccccactgca gtcctttcca gattctattg attacgtcct ggcgaaatgc cggtcttacg
     3421 aattggactg tgtagtacgc gaagacaaag tcagcgggct gcatttcgaa ttcaagaagc
     3481 tcagcacatt cgaattgaat gcgcggatct tctttacatt tgtcgcgagc gacttcgagc
     3541 attccttcta ccctgtccac tccgatcagt ctgatatctt ttccagcgtg ccgctctccg
     3601 attttgcgga gcaattctcc cgtggaacat cccagatcat aaattgtgga gccatcttgc
     3661 acaaagtagt ctgaaacctt ggcgatgatt tcgtgcccct ctgcgtacag aggcacagat
     3721 ttgcttacat gagcatcaaa
//
LOCUS       DAOQJL010000391         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15299_length_3740_cov_4.470828, whole genome shotgun sequence.
ACCESSION   DAOQJL010000391 DAOQJL010000000
VERSION     DAOQJL010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15299_length_3740_cov_4.470828"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1004)
                     /locus_tag="PLG78_14825"
                     /pseudo
     CDS             complement(3..1004)
                     /locus_tag="PLG78_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
     gene            complement(1198..1707)
                     /locus_tag="PLG78_14830"
     CDS             complement(1198..1707)
                     /locus_tag="PLG78_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNE24446.1"
                     /translation="MKKGQDKAEKTGFDVGDYVVYPMHGVGQINAVQKQEILGKKTKC
                     YILDITASKMKVMIPVDKADTIGIRPIIKKKDVKRVLDLLRKDEMDTEEDWKIRYQNN
                     MTKIKSGDIYSVAEVCRNLYKRARDKELSLMERRLYESAYNLVSSEIAISREISMEEA
                     GNLISEVLS"
     gene            complement(1861..3165)
                     /locus_tag="PLG78_14835"
     CDS             complement(1861..3165)
                     /locus_tag="PLG78_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE24447.1"
                     /translation="MHAEAFRNSFIAANRGKPLFLIRFEDIQGMQLLEFVNFLRNEVH
                     SILDITDVEFGFHYMEHPACLLMGIAPIRGWSGPGFPNVDSSVGRFHIECMSRHICSF
                     HFGVGRSQCNFISDEEEIFEELFRTSEKNLRDNLTRWSWTYFNRANTYISDETTDAMI
                     QPTIYYDPRSATYSVNGGEVFLGGGVYGTYRELISDIPPDQDLNRIELLILEKLITAC
                     ERAPGLLKFNISPQSLIDTFAIREKADRLHQLILSRGLNPRNLRLELVEKPYEESRQS
                     LKEVCAHFFNFGITFAADDFGVKSQSHQVVLELGVMIKEFKLDPISFRFKVEEDQTKF
                     LDNLAFIDYCKRLADNREAGITAEAVEDFDTLRFLMEHQIFQYQTNLFAGKMTISDYR
                     ARFNEMKNLPESAVRTILTNRDLMDQVRAKGDIFQVAAQLAR"
     gene            3340..>3740
                     /locus_tag="PLG78_14840"
     CDS             3340..>3740
                     /locus_tag="PLG78_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24448.1"
                     /translation="MSRLHGVLVFAAIVAFSAACLKKASTAVPVETAAISLLVVGDVT
                     ADGQPVKTGQLLKGSPWIIVGPASIMDLQVRDSEQGVVFRLKENSKFRLSSLDRGNGK
                     TFVAQLQSGQLLASVKKRSADEEVRINTPTAL"
BASE COUNT          816 a          952 c          917 g         1055 t
ORIGIN      
        1 aattagcggc gtctgccgcc tttggaaaat tcgcggtcgc tattgtagtc gcgatctccg
       61 ccgttgccgc ggtttccgcc gccttcgcga cctccaccgt agcgttgatt tccgccgccg
      121 ttgccgccgc gctgtttgtt tttatcttcg cgttcgtcat cgtctacagt tccagtagcg
      181 cgagtgaata tcatttttcc tgcggcagtc tgaatgatag aagtgacgtt cacttttact
      241 gttttaccca ggagctggcg gccgttttca acaacgacca ttgtgccatc ttccagatat
      301 ccaattcctt gattttcgtc tttgccttct ttgatcacgt cgatgatgat ttcctctccg
      361 ggaagaacca ctggctttaa agcgctggtc agagtgttga ggtttactac ttcaacgccc
      421 tggagttctg caaccttgtt caaattgaag tcgtttgtaa tcaatttcgc gccggtttcg
      481 cgtgcaaggc gaacgagttt cgcatccaca tcgcgcgcat ccgcgtaatc caggtaactg
      541 atcttcactt ttacattgtc taactgctgg agtttgttga gcatttccag tccgcgtctt
      601 ccgcgattgc gtttgactgg atccggtgaa tcgctgatga gctggatttc gcggagaaca
      661 aagtttggaa tgatgatcgg gccttcgatg aaattcgttt gcgcgatatc cagaatacgt
      721 ccgtcgatta caatagaagt atcgaggatt ttttcctttg ttccgccttc tggcttttca
      781 ggtgtgaaag cagatgcggc aatcgctgtt ccaggtctgc gaccacccat tctacctgct
      841 tctcgcccaa ttacaattcc tgccacgacc agactgattg tgaacgcagg tacatagaga
      901 ttgagatcac tggaaagaat atgcgcgagg aaatacgaag cagcaactcc caggagtgcc
      961 ccgatcgcac tgtggctcag cacgtccgta ttgattcgcg gccagattct atccatgaag
     1021 attgccaaac ctgaggcgat gagcgtgata gccgctccga ttcctgcctc aatgtagttt
     1081 gctccgcctt caaatcgcat ccataagaat gcgccggcgc caaccgcgac aacggtggct
     1141 gccctcaaaa aaactttcat aacaactacc cctccggtcc ggggccgtca ggcagaatca
     1201 ggacagaact tccgagatca aattgccagc ttcttccatg gagatttccc ttgagatagc
     1261 aatttcgctc gatacaagat tataagccga ttcgtacagc ctgcgttcca tgagcgatag
     1321 ttctttgtca cgcgcacgct tgtagagatt gcgacagacc tctgccacgg agtaaatgtc
     1381 tccggacttg atcttggtca tgttgttttg ataacggatt ttccaatcct cttccgtatc
     1441 catctcgtct ttcctgagaa gatccagtac tcgcttaacg tctttcttct taataattgg
     1501 acggattccg atcgtgtcgg ccttgtccac cgggatcatc accttcatct tggaagcagt
     1561 gatatccagt atgtaacatt tggtcttctt tcccaggatc tcttgctttt gcacggcgtt
     1621 gatttgtcct actccgtgca ttggatagac tacataatct ccgacatcaa aacctgtttt
     1681 ttcggctttg tcctggccct ttttcacttt tgcctgcggc atatctatgg accgattttc
     1741 ctagtcatct actatattga tgtttagaca ttgtgtcaag aagtttttaa atggagccgc
     1801 cccggcggca tctatcctca taaactggat cctggacgcc catgggaagg ctgcccggag
     1861 tcacctggcg agctgggctg ctacctggaa tatatcgcct tttgctctga cctgatccat
     1921 gagatctcga ttcgtgagaa tggttctgac tgctgattcc ggtaggttct tcatttcatt
     1981 gaatcgcgct ctgtagtccg agatggtcat tttccctgcg aatagatttg tctggtactg
     2041 gaagatctgg tgctccatca gaaaccgcag cgtatcaaaa tcttccaccg cctccgctgt
     2101 gattcccgcc tcgcggttgt cagcaagcct cttgcaataa tcaatgaatg ccaggttgtc
     2161 cagaaatttg gtctggtcct cttctacctt gaatctaaaa ctgattggat cgagtttgaa
     2221 ttccttgatc atcaccccaa gctccagaac tacctggtga ctctggctct tcactccaaa
     2281 atcgtctgcc gcgaatgtaa tcccaaagtt aaagaagtga gcgcatactt ctttgagcga
     2341 ttgtctgctt tcttcgtacg gcttttctac gagctccagt ctcaggtttc taggattgag
     2401 tccccgggat agtatgagtt ggtgaagtcg atctgctttt tctctgatag cgaatgtgtc
     2461 tatgagcgat tgcggagaaa tgttgaactt caaaagcccg ggtgctcttt cgcatgcagt
     2521 gatgagtttc tctaaaatca gaagttcgat tcgattcagg tcctggtcag gcggaatgtc
     2581 gctgatgagt tctcggtagg ttccgtacac tcctcctccg aggaatactt ctcctccatt
     2641 tacagagtac gtagcgctgc gaggatcata gtatatggtt ggctggatca tcgcatccgt
     2701 tgtttcatcg gagatgtatg tgtttgcgcg attgaaataa gtccagctcc acctcgtgag
     2761 attgtctcga agattcttct ctgacgtgcg aaacagttct tcaaatatct cttcttcatc
     2821 cgagatgaag ttgcactggg atcgcccaac tccaaaatga aaagagcaga tatggcggga
     2881 catgcattcg atgtgaaacc tgcccacgga gctatctaca ttcgggaagc ccggaccgct
     2941 ccagccgcga atgggtgcga ttcccatcag gaggcaggcg ggatgctcca tgtagtggaa
     3001 cccgaactct acatccgtga tgtcgagtat gctgtgaact tcgttgcgca gaaaattgac
     3061 aaactccaga agctgcattc cctgaatgtc ttcgaatcgg atgaggaaga gaggtttacc
     3121 gcgattggcg gctatgaaac tgttccggaa tgcttccgcg tgcatcagac cgaagtaacg
     3181 atcgtgcgaa ccatccttcg atgagctttc gtccataacc gactggagcg tggatctctg
     3241 aggaggcatg tcaaatgttt tctggttgac caggccccga agcaggttca cctttgggcg
     3301 tctggcgggc gtcgcgtcgg gggaaggtag gtttcgcata tgtctaggct gcatggtgta
     3361 ctcgtttttg ccgcaatcgt cgctttttct gcggcctgtt tgaagaaagc ttcgactgcg
     3421 gtgcccgtag aaacggccgc catttccctg cttgtcgtag gagacgtaac cgcggacgga
     3481 caaccggtga aaaccgggca gcttctgaaa ggcagcccgt ggatcatcgt gggtccggcc
     3541 tccatcatgg atcttcaggt tcgggattcg gaacagggcg ttgtctttcg cctgaaagaa
     3601 aatagcaaat ttcgtctgag ttcactcgac cgtggaaatg gaaagacgtt tgtagcgcag
     3661 ctccagtcag gtcagctact tgcctctgtg aagaagagaa gcgcagatga agaggttcgc
     3721 atcaacacgc caactgctct
//
LOCUS       DAOQJL010000392         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15318_length_3738_cov_3.713006, whole genome shotgun sequence.
ACCESSION   DAOQJL010000392 DAOQJL010000000
VERSION     DAOQJL010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15318_length_3738_cov_3.713006"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..516)
                     /locus_tag="PLG78_14845"
     CDS             complement(91..516)
                     /locus_tag="PLG78_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE24449.1"
                     /translation="MKNALLWLEDHKDWGIEMLRMYLGVVLILKGFYFIRDMNSLLAL
                     IGLQSSLWLGGIFAHYIVLAHLIGGLFVIIGLLTRIVAAFQVPILLGACYVVFNHDGI
                     LSASSNFEFTSLVLILLVMFVFYGGGRLSVDYFLGKPKD"
     gene            complement(647..1567)
                     /locus_tag="PLG78_14850"
     CDS             complement(647..1567)
                     /locus_tag="PLG78_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE24450.1"
                     /translation="MARRRKFNRALILSGGGARGAYQVGVWKYLQELDWKPDLICGTS
                     VGSVNTAAIACGLNLEELINLWKTIERGRIYRLSLWRQLVHVLTRRGYAPLMDTEPLK
                     NLLLDKLDVTKLRHSEYEIIITATNILKSQLKFFSQRVIDIEHVMASSAIPILFPWQY
                     IDGEPYWDGGIMANTPLLPALERGCKEIIVVLLSPVGGARLSPPATRRQALERVFEMS
                     LIGSYEAFMSHLAYEYKQRSQQSVIRKLIRRTLTLGDTRIATVAPGQMLGFYSILNFS
                     ARQADTLIHEGYMDARNQLSQFFGLDEAPY"
     gene            complement(1567..2010)
                     /locus_tag="PLG78_14855"
     CDS             complement(1567..2010)
                     /locus_tag="PLG78_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24451.1"
                     /translation="MKRFFLLFMLLSLPVGAQEIRGLDKEYYNAYLDSLPNEKKKRLE
                     TLKKNLLSMLKKVIPREDPDHGQSYAEKLTVESFQFEEISAQSPFVRGMMKEIPGLRP
                     DKFMFVVKTGPYLVYVAFNVNPGQFITSESQQHVIIRAVDEGHPN"
     gene            complement(2046..2816)
                     /locus_tag="PLG78_14860"
     CDS             complement(2046..2816)
                     /locus_tag="PLG78_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE24452.1"
                     /translation="MIRYFVTLTFSILFFQTAAFSHPASLLVAEEGGRVFVSENTAIV
                     RPMASLTKIGSALFAVSLSPLDRWIRLSETVRSRGPDDRSAPLRLQEQYTLRDMLSAM
                     LISSTNAASRSVAKELAGSESEFVRRLGEFGARRGWKQTRFADSHGLSPASISSVDDI
                     SRMMDDFIRNPDLMKVLESVNPVIHEKQGRTIGLLSTLDLREYRGFRISGKTGRTTAA
                     GQCFAGFAVLGSKRYRIIFLGSSDMQADIRAAVDQIKK"
     gene            complement(2813..>3738)
                     /locus_tag="PLG78_14865"
     CDS             complement(2813..>3738)
                     /locus_tag="PLG78_14865"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24453.1"
                     /translation="KEGPETGYFPDGSIRYTGQNSADKKTGHWKIKADATKGCESEGD
                     FENDLKTGRWVECKGNIKEPFIEFEGSYVQGLRDGPGKFLNSEGKVSSQGDFRADLGC
                     KAALKPGASKQDFDRCAKRTGHWVYYHSNGEKSTEGDFDPATGLETGTWHEFYRSGEK
                     LGMGPRTGDRKGLWTFYGKDGSILYQLEFAGSDFLPKTAIFWKDGKKSAQGPLSMGMF
                     KFSQDKDAVELTSPIKDGIWTEYYPNGQKSGEGNYTSNRRDGKWTLYDESGKKIGEGP
                     YMMDKKDGEWLELEGGAMVKKKYQFGKPARF"
BASE COUNT          949 a         1019 c          854 g          916 t
ORIGIN      
        1 caggtgcgca ggattttcgt gcgaattttc gcgcgcattc ccatgtgaag aatttcgcac
       61 cgcgaaattc tctcaatcgc cggcgccaca tcagtccttt ggtttgccca ggaagtaatc
      121 cacggacaac cttcccccac cgtaaaacac aaacatcaca agcaaaatca gcacgaggga
      181 agtgaattcg aaattcgagg aagcggacag aatcccatca tggttgaaaa ccacatagca
      241 ggcacccagc aaaatcggca cttgaaaggc agcgacgatc ctggtcagaa gaccgatgat
      301 cacaaacaga ccaccgatca ggtgagccag aacaatgtag tgagcgaaaa ttccacccag
      361 ccacaaagag ctttgcaggc caatgagagc gagcaaagaa ttcatgtcac gaatgaagta
      421 aaaacctttg agaatcaaaa ctactccgag gtacatacgg agcatttcga ttccccagtc
      481 tttgtgatct tccagccaga gcagcgcatt tttcatacag cctcccggtg ccgggggcgc
      541 aatgcagcag agcctcaggt gcatccgcaa aaccaggcac cctgaacgcc ctcagtataa
      601 ctcgacccta aaaggaggca agattaaatt atgaatttta agatcactag taaggcgctt
      661 cgtccaggcc aaaaaactgc gatagctgat tgcgcgcgtc catgtaaccc tcatgaatga
      721 gagtgtccgc ctgacgggcc gagaaattca aaatagaata aaatccaagc atttgccctg
      781 gagcaaccgt tgcaatgcga gtgtccccca gagtgagagt tctacgaatc aatttgcgta
      841 ttacgctctg ctgcgatctt tgtttgtact cgtaggcgag atgggacatg aatgcttcat
      901 aggatccaat caaagacatc tcaaaaacac gctccagggc ctggcgtctg gttgcgggag
      961 gagacagcct ggctccaccg acaggagaaa gcagaaccac aatgatttct ttgcatccgc
     1021 gttcgagcgc aggaagaagc ggagtatttg ccatgattcc accatcccag taaggctctc
     1081 cgtctatata ctgccacgga aagagaatgg ggatggcact ggacgccatc acatgttcaa
     1141 tatcaatgac tctttgagag aagaacttca actgcgattt taaaatgttc gttgctgtga
     1201 tgatgatttc gtattcgctg tgacgcaatt tggttacgtc cagtttatcc aggagcaaat
     1261 tcttcagtgg ttctgtgtcc atgagtggag cataacctct cctggtgaga acatgaacca
     1321 gctgtctcca cagactcaat cgatagattc ttccgcgttc gattgttttc catagattga
     1381 tcaactcttc tagattcaat ccgcatgcaa tggctgccgt attgacagat ccaaccgaag
     1441 tcccgcaaat gaggtccggc ttccagtcca gttcctgaag gtacttccac acgcctacct
     1501 gataggcgcc gcgagcccca ccacccgata gaatcaaagc gcgattgaat tttctacgac
     1561 gagccatcag tttggatggc cttcatccac tgcgcgaatg atcacatgct gctgcgattc
     1621 ggaagtgatg aactgaccag gatttacgtt gaaggcaaca tacaccagat agggtcctgt
     1681 tttgacgacg aacatgaatt tgtccgggcg caagccagga atttccttca tcatccctct
     1741 tacaaacgga gactgcgcgg agatctcttc aaactgaaaa ctctccacag tcagcttctc
     1801 cgcatacgat tgcccatgat ctggatcttc tcgcgggatt actttcttga gcatgctgag
     1861 caaattcttc ttcaatgtct caagacgctt cttcttctcg ttggggaggc tatccagata
     1921 cgcattgtaa tactctttgt ccaggccgcg aatttcctgg gcgcccacag gaagagaaag
     1981 aagcatgaag agcaggaaga atcgcttcat cggtggagaa tccaacgctc gcccttccct
     2041 gcaatctact tttttatctg atccacggcg gctcgaatgt ctgcctgcat atcagaagag
     2101 cccagaaata tgatccgata gcgcttgctt ccgaggactg cgaaaccggc aaagcactga
     2161 ccggccgccg tggtcctccc cgtttttccg gagatgcgaa agcccctgta ctcccgaaga
     2221 tccagggtag acagaagtcc gatcgtgcgg ccctgcttct cgtggatcac aggattcacg
     2281 ctctcgagca ctttcatcaa atcagggttt cgaatgaagt cgtccatcat gcgagatata
     2341 tcgtccacgg atgaaataga agcaggagaa aggccatggc tgtccgcaaa cctggtttgt
     2401 ttccagccgc gacgagcccc aaattcgcct aacctgcgaa cgaattctga ttccgaaccc
     2461 gccagttctt tagcaacaga tctggatgca gcgttcgttg agcttatgag catcgcagat
     2521 agcatgtcgc gcagagtgta ttgttcctgc aaacgaagcg gggcacttct gtcatctggt
     2581 ccgcgacttc gcactgtttc gcttaggcgg atccagcgat cgagagggga tagactcaca
     2641 gcgaaaagag cagagccgat tttggtgagg ctcgccatgg gcctgacgat tgcagtgttc
     2701 tccgagacga agacccggcc accctcctct gcaacaagaa gcgaagccgg atgcgaaaac
     2761 gcggccgtct gaaaaaacag aattgagaaa gtaagagtta cgaaataccg aatcaaaaac
     2821 gagcgggctt cccgaactga tatttcttct tcaccatggc tccaccttca agctccagcc
     2881 attctccatc tttcttatcc atcatgtatg gaccttctcc gattttcttt cctgattcgt
     2941 cgtataacgt ccatttccca tcacggcgat tgctggtata attcccttct cctgatttct
     3001 gcccgtttgg atagtactct gtccagattc catctttgat ggggctagta agttccactg
     3061 catctttgtc ctggctgaac ttgaacatgc ccatcgaaag aggaccctgc gcggattttt
     3121 tgccgtcttt ccagaagatg gcggtctttg gcagaaagtc acttcctgca aattcaagtt
     3181 gatataagat cgaaccgtcc tttccgtaaa acgtccacag gcccttccga tccccagtgc
     3241 gaggacccat ccccagcttt tcaccgctac gatagaattc gtgccatgtt cctgtttcca
     3301 gacctgtggc cggatcaaaa tccccttcgg ttgatttctc tccatttgaa tgatagtaca
     3361 cccagtgacc tgtgcgcttc gcgcatctgt caaagtcctg cttgcttgca ccgggcttca
     3421 gcgctgcctt gcagccaaga tcggcacgga aatctccctg cgaactcacc ttgccttcgc
     3481 tgttcaggaa tttaccagga ccatcgcgca agccctgaac atagcttccc tcaaattcga
     3541 taaatggctc tttgatgttt cccttgcatt ccacccagcg acctgttttc aaatcgtttt
     3601 caaaatcgcc ttctgattca catcccttcg tcgcatccgc tttgatcttc cagtgcccgg
     3661 ttttcttatc cgcagagttc tgacccgtgt aacgaatgga cccgtccgga aaatatcctg
     3721 tctcaggacc ttctttta
//
LOCUS       DAOQJL010000393         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15476_length_3717_cov_4.949208, whole genome shotgun sequence.
ACCESSION   DAOQJL010000393 DAOQJL010000000
VERSION     DAOQJL010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15476_length_3717_cov_4.949208"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLG78_14870"
     CDS             complement(<1..564)
                     /locus_tag="PLG78_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE24454.1"
                     /translation="MTKKKFLGWIANAILLCGFPLFAEDLVQVASGFKDPTQVVFSRR
                     AGMCVVEQRGIITCSGKPYLDLTSTVRSGGEMGLLSMAFAPDFEKSQSSWVYVNYTSG
                     NPLWTNVAAIPVRGSPEPDKKRVILRFAQPFNNHNGGLLPFGPDGSLFIGTGDGGSAG
                     DPYNNAQRRDNYLGKILRIIPTPEGMSS"
     gene            640..1434
                     /locus_tag="PLG78_14875"
     CDS             640..1434
                     /locus_tag="PLG78_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE24455.1"
                     /translation="MVRWNFLTDSLETGALYLDCRSEEQYKLASIKDAVGCGFIKKPY
                     GSGASSLAKLGGFLADVRKKTESKSTVICFDEGEGMYACRLAWILIGMGVNAKVLAYK
                     FDDLPKEWRAPGKEQLASPPGKAVPVKSLTTISQLQKDLTRVQLIDSRTMEEYEGRMP
                     RMTNPDVGGICGRIPGSVHWDWLMLYDAEGKLRAREEVMAQIRDIGLIPERPTVIYDY
                     NGARSCTTALVLTRCGYRQVSVYLGSWMEWRKTDLPKQHVRVWGEE"
     gene            1431..3512
                     /gene="metG"
                     /locus_tag="PLG78_14880"
     CDS             1431..3512
                     /gene="metG"
                     /locus_tag="PLG78_14880"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNE24456.1"
                     /translation="MRKLLVTSALPYANGPIHLGHLVETIQTDIWVRFQKLCGHEAYY
                     FCADDTHGTPIMIAAKKAGKTPEEFTAEIQKQHYRDFNGFFINFDNYYTTNAPENREF
                     SELIYEAAKKKGNIARKTIRQLYSEVDGMFLPDRFVKGTCPKCKSPEQYGDSCEVCGS
                     TYSPTDLIDPKSTVSGDTPVLKETDHLFFKLGDYQKFLETWLGKGVSPGIKRKLDEWL
                     TDGLRDWDISRDAPYFGFAIPGEKDKFFYVWLDAPIGYMASSRNFFNQNDPSRWDEFW
                     KDGSGEIHHFIGKDIVYFHTLFWPALLEAGGFQKPARVHVHGFLTVNGEKMSKSRGTL
                     VRAENYLKHMEPEALRYFYASKLGPGLDDLDLSRDDFLSRWNADIVGNITNIYSRLCG
                     GILPKLGSTLSKSISKEGTALKKEGMAAADKVKQHFDTLEYAKAIKEISAFADSINRF
                     VASKEPWKTIKTNPEEARQVVTDALNSARILAGLLKPVVPSFVRGVENLLNLKEEITF
                     QNLDQKLPSEHIIHPYRNLAGRMDEKVFDAMLEEEARLAAAEKPAASAEKTAPETGAK
                     SQSKASEKPAEKAGDQAKSGLISIEDLEKVELRAGRILHAELVEGADKLLRVELDVGE
                     AEPRQVFAGIRAAYKPEDLTGLTVACVANLIPRKMKFGISSAMLLASGKDAELTLYTA
                     HRQAKPGDRLK"
     gene            3529..>3717
                     /locus_tag="PLG78_14885"
     CDS             3529..>3717
                     /locus_tag="PLG78_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24457.1"
                     /translation="MRPLLRTLASVSPIVATAITFLLVSPLFAQITPGLGDWRTHSTP
                     HFRIHYQKADEELAHRMAG"
BASE COUNT         1022 a          933 c          940 g          822 t
ORIGIN      
        1 ggaactcata ccttcggggg tcggaatgat tcttaaaatc ttgcccaggt aattgtccct
       61 cctctgcgca ttgttgtagg ggtcccccgc cgatccgcca tcccctgtgc caatgaaaag
      121 agaaccgtcc ggtccaaagg gcaagagtcc accgttgtgg ttgttgaagg gttgagcgaa
      181 tcttagaatc actcgctttt tgtccggttc cgggctccct cgaaccggaa tcgcagccac
      241 attggtccag aggggattgc ccgaggtgta gttcacgtag acccaggaag actgcgattt
      301 ctcgaaatca ggcgcaaaag ccatggaaag aaggcccatc tcgccgccgg agcgaacagt
      361 ggaagtcaaa tcaagatacg gtttgccaga gcatgtgatg atgccgcgct gctctacgac
      421 gcacataccc gcgcggcgcg aaaatacgac ctgcgtggga tccttgaaac cggaagcaac
      481 ttgcactaga tcttccgcga acaacggaaa accgcaaagc agaatcgcgt tcgcaatcca
      541 gcccaggaat ttttttttgg tcataggatt tttttaattc taaaaaaggg agtgcttctt
      601 tttgaaagca aattcctgta tcaagaaaaa ggaaacagat tggttcgctg gaattttctc
      661 actgatagcc tggaaacagg cgcgctctat ctcgattgca gatcggaaga gcaatacaaa
      721 ctcgcttcga tcaaggatgc agttggctgc ggctttatca agaaaccgta cggatccggc
      781 gcaagctcgc ttgcgaagct cggcggattt ctcgcagacg taagaaagaa gacggagagc
      841 aagtctaccg tcatctgctt cgatgaaggc gaaggcatgt acgcatgcag gctcgcgtgg
      901 attctcattg gaatgggagt gaatgcgaaa gtgctcgctt acaagttcga cgatcttcca
      961 aaagagtggc gcgcacctgg aaaggaacaa ctcgcatctc ctcctggaaa agcagtccct
     1021 gtaaaatctc tcacaaccat cagccagctc cagaaagatc tcactcgcgt gcagctcata
     1081 gacagcagga ctatggaaga atacgaagga agaatgcctc gcatgacaaa tccagatgtt
     1141 ggtggaatct gcggacgcat acctggaagt gttcactggg actggctcat gctctacgac
     1201 gcggaaggaa agcttcgcgc acgcgaagag gtgatggccc aaatcagaga catcgggctc
     1261 attcctgaaa gaccaactgt catttacgac tacaacggtg ctcgttcatg caccaccgct
     1321 ctagtgctca ctcgctgtgg ctacagacag gttagcgtgt acctcggatc atggatggag
     1381 tggagaaaga cagatcttcc caaacagcac gttcgggtat ggggtgagga gtgaggaaac
     1441 tactcgtcac ttcagcactt ccttacgcaa acggccccat ccacctgggc cacctggtag
     1501 agaccatcca gacggacatc tgggttcgat tccagaagtt atgcggccat gaggcgtact
     1561 atttctgtgc agatgacact catggaacac ccatcatgat tgccgcgaaa aaagcgggaa
     1621 aaacaccgga agaattcaca gcagaaatcc agaagcagca ctatcgtgat tttaatggat
     1681 tttttataaa ttttgataac tattacacaa ccaatgcccc ggaaaatcgc gagttttccg
     1741 agctgatcta cgaggcggca aagaagaaag gcaacattgc tcgcaaaaca atccgccagc
     1801 tctacagcga agtggatgga atgtttctgc cagatcgttt tgtgaaaggt acatgtccta
     1861 agtgcaaatc tccggagcag tatggagatt cctgcgaagt gtgtggctct acctactctc
     1921 ccacggacct catcgatcca aaaagcacag tcagtggaga cacgcccgtg ctcaaggaaa
     1981 cggatcatct cttttttaaa cttggcgatt atcagaagtt tctcgaaacc tggctgggca
     2041 aaggcgtatc tcccggaatc aagagaaaac tggacgaatg gctgacagac ggcctacgcg
     2101 attgggatat ttcgcgcgat gcgccttact ttggttttgc gatccccggt gaaaaagata
     2161 aattctttta cgtatggctg gatgcaccca ttggctacat ggcttcttcg cgcaatttct
     2221 ttaatcaaaa cgatccttcg cgctgggatg aattctggaa agacggatcc ggtgagatcc
     2281 atcacttcat tggaaaggac attgtttatt ttcatactct attctggccc gcgcttctgg
     2341 aagcaggcgg ttttcaaaaa ccggcgcgcg ttcatgtgca cggttttctc actgtaaacg
     2401 gggaaaagat gtccaagagc cgcggcacac tggttcgcgc tgaaaattat ctgaagcaca
     2461 tggagccaga ggctctacga tatttttatg cgtctaaact tggcccggga ctcgatgacc
     2521 tggatctttc acgcgacgat ttcctttcac gctggaatgc agacattgtt ggaaacatca
     2581 caaacattta ctcgcggtta tgcggcggca tccttccaaa gcttggatcc acactctcaa
     2641 aatccatttc aaaagaaggc actgctctaa agaaagaagg gatggccgcg gcagataaag
     2701 tcaaacagca ctttgacact ctggaatatg caaaggcaat caaagagatt tctgcttttg
     2761 cggatagcat caatcgattt gtggccagca aagaaccctg gaagaccatc aaaaccaacc
     2821 cggaagaagc ccgtcaagtg gtcacagacg cattgaactc tgcccgaatt ctagcgggcc
     2881 tactcaagcc agtcgtccct tcctttgttc ggggcgtgga gaatttgcta aatctaaaag
     2941 aagaaattac tttccagaat ctagaccaga agctcccttc ggaacatatc attcatccct
     3001 acagaaatct ggcgggaaga atggacgaaa aggtatttga cgcaatgctg gaagaagaag
     3061 caagacttgc ggcggcagaa aagccggccg catccgcgga aaaaacagca cctgaaacag
     3121 gcgctaaatc acagagcaaa gcctctgaaa agcctgcgga aaaagctggg gaccaggcaa
     3181 agtcaggact catctccata gaagatctgg aaaaagtaga actgcgcgcg ggaagaattt
     3241 tgcatgcaga gctggtggaa ggtgcagaca agttgctccg cgtggaactg gacgtgggag
     3301 aagcggaacc acgccaggtg tttgccggaa ttcgcgccgc ttacaaacca gaagatctaa
     3361 ctggactcac agttgcgtgc gttgccaatc tcattcctcg aaaaatgaaa ttcggaatct
     3421 caagcgccat gttactcgcc tccggaaagg acgcagaact gactctctat acggcccacc
     3481 gccaggcaaa gcctggcgat cgattgaagt aatccggtga aggagcagat gcgcccactg
     3541 ctcagaacgc tcgcttctgt ttcgccgatt gttgcgacag cgatcacttt ccttcttgtt
     3601 tctcctctgt tcgcgcagat caccccgggt cttggagact ggcgcactca ttcgactccg
     3661 cactttcgca tccactatca gaaagcagac gaagaacttg cacatcgcat ggcgggc
//
LOCUS       DAOQJL010000394         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15504_length_3714_cov_3.655644, whole genome shotgun sequence.
ACCESSION   DAOQJL010000394 DAOQJL010000000
VERSION     DAOQJL010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15504_length_3714_cov_3.655644"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLG78_14890"
     CDS             <1..722
                     /locus_tag="PLG78_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE24458.1"
                     /translation="RLHIEGTRAILEGAKKAGVRRVVLASTSGTVGIFEDERTADDNS
                     PYAMAVASEFAYYRSKIYQEKLAMDFAKSSDLELVCLRPSLLLGPGDHRLSSTGDVKA
                     FLDKKFPVIPAGGISFVDARDTAAAFVEAAVRKIAPLPRTYLLTAENWSMARFVREME
                     EVSGVRAPTKQLNKSILDIGSSIVKLLGEPSFLPDAESMKMASLYWYVDATRAKEELG
                     FNTRPARETLRDTIDFIRAMP"
     gene            complement(746..3508)
                     /locus_tag="PLG78_14895"
     CDS             complement(746..3508)
                     /locus_tag="PLG78_14895"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE24459.1"
                     /translation="MAKKKTTVESHDFSVLETLRGKTLFVIGGTGFLGRVMLYYFVKY
                     VPDVKLVLLVRPTHGRTGQDRLEKEVLNSPVFTTNPEDKDLRKRALAMIEVVDGDATR
                     PGLGIPSEKSAKIAERIDAVLNTAGNVEFNPPLDLSLSANTLATMQALEFAESTRGKK
                     YVHISTCYVADRTVHRDYAPEIPVASTIVTSRGNEIRIDVQKEIEASRAKIEELKAEF
                     DGEPRMAEFRERAARELKKMGRADGDRMVDKIAKNIRTFELREALTRAGKERAERINR
                     PNVYTYTKTLAELLVLAKTEVESVIVRPSIVEASVRTPFVGWNEGIQGSAPIIFMMHR
                     GHRMIPSLSKNPGEREDAVLDLIPVDFVAAGTILALSALLRGKARQVYQLAAGPIRTP
                     MTITRCLHISQTTLRDLIREEDTGLKRWARLNMQAVTVSRDTFEKFSSPRTLKVLEKL
                     KSRTEEMEEKASGPAQRILSSVSRNINKFYNVSMLKNRMFQEFMPFINHGFPIFQNRN
                     AIELCQELPAEERSIFQFNPHELDYIEYFSEIHIPGVVKFVFPVLEKRFQAALKAGGD
                     SSDKGVFEAFRSLFTGGDFREKFERLKDAATSGSSKKAESKASESSSKTQKEKTHYLA
                     VHAPRFEAGHLKDWKDENLHSFADHVELITGVRIGVPELRELRTPARLESYVEKVLAE
                     KPPAPGKPFRFKLPKDGLNIPGWAADPTRGFLYRLQMWFYKNVLHTTIRGQKNIPWNN
                     HHVIVVSNHSSHLDYGLVQYSLGKFGQHMGILAARDYFYTNFWKSTFFTHLMTTIPVE
                     REAQGGYGQALKHAISFLNKGGPLLIFPEGTRSDDGQIQPFKHGLGYLVHHTRADVLP
                     VQISGTHKALPKGATLLRGRKVSVQIGNVISYEDLEEDTKGLSPTKTYDVIAKKIERA
                     VRSGTH"
     gene            complement(3512..>3714)
                     /locus_tag="PLG78_14900"
     CDS             complement(3512..>3714)
                     /locus_tag="PLG78_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24460.1"
                     /translation="YWGETGRRKVGQVIAVGAEDKHVPERMGWQNATTFEAALEMAKK
                     SVGPNPNIVMLHQPPVVVARLV"
BASE COUNT          876 a          964 c          894 g          980 t
ORIGIN      
        1 tgaggcttca tattgaaggg actcgcgcca ttcttgaggg agcaaaaaaa gctggagtgc
       61 gcagagtggt gctcgcatcc acatctggca cggttggaat ctttgaagat gagagaaccg
      121 cagacgataa cagcccgtat gcgatggcag ttgcatctga attcgcgtac tacagaagta
      181 agatctatca agagaaactc gcgatggact ttgcaaaatc gagcgatcta gagctcgttt
      241 gtctgcgacc ttcccttcta cttggcccag gggatcatcg tctcagttcc accggagatg
      301 taaaagcgtt ccttgataaa aagtttcctg tcatcccggc gggcgggatc tccttcgtgg
      361 acgcaagaga cacggcggct gcattcgtag aagcggctgt gcgaaaaatc gcccctctcc
      421 ctcgcactta cctgctgact gcagagaact ggagcatggc caggtttgtg cgcgaaatgg
      481 aagaagtgtc cggagttcgc gctcccacaa agcagctcaa taaatcgatt ctagacatag
      541 ggtccagcat agtgaaactt ctcggagagc catcctttct gccagacgca gaatctatga
      601 agatggcctc cctttactgg tacgtggatg cgacgcgcgc gaaagaagag ctcggattca
      661 atacgcgccc cgcgcgcgaa acgctgcgag acacgatcga tttcattcgc gcgatgcctt
      721 gattcgattc agtcgctcag gaatatcaat gagttcctga acggactgct ctttcgattt
      781 tctttgcgat cacgtcgtat gtcttggtgg gagaaagtcc ttttgtatct tcttccagat
      841 cctcatacga aatgacattt ccaatttgca cggacacttt tcttccgcga aggagagtcg
      901 cgcctttggg aagagctttg tgtgttccag aaatctgaac cggaagaacg tcggccctgg
      961 tatgatgcac caggtaacca aggccgtgtt tgaatggctg aatttgtccg tcgtcggacc
     1021 tggttccttc tggaaaaatc aagagcgggc cgcccttatt tagaaatgaa atcgcatgct
     1081 tcaatgcctg gccgtaacca ccctgcgctt ctctttccac aggaatcgta gtcatcaagt
     1141 gggtaaagaa tgtggacttc cagaaattcg tgtagaaata atcgcgagcg gcgagaattc
     1201 ccatgtgctg tccaaatttt ccgagcgaat actgaacgag accgtagtcc agatgactcg
     1261 aatgattgga tacaacgatg acgtgatgat tgttccacgg aatatttttc tgaccacgaa
     1321 ttgtagtgtg gagcacattc ttatagaacc acatctgaag gcgatacagg aaacctctcg
     1381 tcggatcggc agcccatccc gggatattca atccatcttt gggtagcttg aagcgaaatg
     1441 gtttgcctgg tgccggtggc ttttctgcga gcactttttc tacgtaggac tccagtctcg
     1501 cgggagtgcg aagctccctg agttctggca ctccaatgcg aacgcccgtg atcaattcaa
     1561 catggtctgc gaaggaatgg agattctcat ctttccaatc tttgagatgc ccggcctcaa
     1621 acctgggagc atgcacagca agataatgag tcttttcctt ctgtgttttc gaactcgact
     1681 cggacgcctt tgattccgct ttcttcgagc tgccggaggt ggcggcatcc ttcaaacgct
     1741 cgaacttttc gcggaagtct ccgccagtga acagagaacg aaacgcttcg aacactcctt
     1801 tgtcgctcga atcgcctcct gctttgagtg ccgcctgaaa tctcttttcc agaaccggaa
     1861 aaacaaactt tactacaccc gggatatgaa tctctgaaaa atattcgatg taatcgagct
     1921 cgtgcggatt gaactgaaaa atacttcgct cttccgccgg caattcctga cacagttcaa
     1981 ttgcgtttcg attctgaaag ataggaaatc catgattgat gaacggcatg aattcctgaa
     2041 acatcctgtt cttcagcatg ctcacgttgt aaaacttgtt gatgtttcgt gagaccgacg
     2101 aaagaattct ctgcgccgga ccggacgctt tctcttccat ctcttctgtt ctgctcttta
     2161 gtttctccag aactttcaaa gtgcgcggac tcgagaactt ctcgaacgta tcgcgtgaaa
     2221 cagtgacggc ctgcatattc aaccgtgccc atcttttaag accggtgtct tcttcacgaa
     2281 tcaagtcacg cagcgtagtc tgcgatatat gaagacagcg agtgatggtc atgggcgtgc
     2341 gaatgggtcc cgccgcgagt tgatacacct gacgcgcctt gccacgaaga agtgcactta
     2401 gcgctagaat ggttcccgct gcaacgaaat cgaccggaat caaatcgagc acagcatctt
     2461 ctctttcacc tggattcttc gatagagaag gaatcattct gtgtccgcgg tgcatcatga
     2521 aaatgatcgg agcagaacct tgaatgccct cattccagcc gacaaacggt gtgcgaacgg
     2581 acgcctctac gatggacgga cgaacaatca ctgactctac ttccgttttt gcgagaacca
     2641 gaagctctgc cagagtcttt gtgtatgtgt agacgttagg acgattgatt ctctctgcgc
     2701 gctccttgcc tgctcgcgtg agtgcttcgc ggagttcaaa tgtgcgaatg tttttggcta
     2761 tcttgtctac cattcgatcg ccgtctgcgc ggcccatctt cttgagctct ctggcggccc
     2821 gttcgcgaaa ctcagccatc cttggttccc catcgaactc tgcttttagt tcttctattt
     2881 tcgcgcgaga tgcttcgatc tctttttgca catcgattct gatttcgttg ccgcgggaag
     2941 ttacaatggt ggatgcaaca ggaatttcag gagcgtagtc gcgatgcacc gtgcggtctg
     3001 ccacataaca ggtagatatg tgaacgtact tttttcctcg cgtgctttct gcaaactcta
     3061 gagcctgcat ggtggccagc gtattggcgc tcagactcag atccagtgga ggattaaatt
     3121 caacattgcc cgcagtattc aacactgcat caattcgttc tgcaatcttt gctgattttt
     3181 cagaaggaat tccaagacca ggacgagtgg cgtccccatc cacaacttca atcattgcca
     3241 gagcgcgctt gcgcaagtct ttgtcttccg gattggttgt aaaaacaggc gaattcaaga
     3301 cttccttctc cagcctgtcc tggccagtac gtccgtgcgt tggtcgcacc agaaggacaa
     3361 gcttcacgtc tggaacgtac ttcacaaagt agtacaacat aacgcgaccc aggaagcctg
     3421 tgccaccaat gacgaataac gtctttcctc tcaaagtttc cagaacggaa aaatcatgag
     3481 actcaactgt ggttttcttc tttgccatac cttagaccag tcgcgcaaca accactggtg
     3541 gctggtgcag cattacaata tttggatttg gaccaacgga cttttttgcc atttcgagcg
     3601 ccgcttcgaa tgtagttgca ttctgccatc ccattctttc agggacgtgc ttgtcttctg
     3661 ctccgactgc aatcacctgc cctacctttc tacgccctgt ctctccccag tacc
//
LOCUS       DAOQJL010000395         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15554_length_3706_cov_3.061627, whole genome shotgun sequence.
ACCESSION   DAOQJL010000395 DAOQJL010000000
VERSION     DAOQJL010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15554_length_3706_cov_3.061627"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..750
                     /locus_tag="PLG78_14905"
     CDS             250..750
                     /locus_tag="PLG78_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24461.1"
                     /translation="MKSTGEVMGLAPTFEEAYVKAVIGAGEKIPEAGGVFVSVNNIAK
                     QSLATEIKILKDLGYVMYATEGTAEFLKTKGVDVKVLHKLRDQVSPSALDEMINGNLK
                     LIINIPYSQTTRDDAFLIRQEASRRGILCITTVPGVRAFVRGLKQMKNKKFTVNSLQE
                     IQKRIG"
     gene            complement(762..1862)
                     /locus_tag="PLG78_14910"
     CDS             complement(762..1862)
                     /locus_tag="PLG78_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24462.1"
                     /translation="MKFTDIFRRVNHRVREARDRMTEKSREAGDRMRSVIDTFSDEWM
                     ESAFHRYRQMLAPSPDSLPSDFRLRESSLPRRIYERLFPVDHVFRMSWKYGAPFLEDF
                     MPISPDAFAGRGSYKFVYRLPWRMVLKVSKEILPSDPIFGSMYREVARNPERFLSAEE
                     LELLEHLCQGKGSSTKEKLHFKFHRLGMERYHYWRVREALPDLVLPTRFFMGKRYRKH
                     FVTEGFSEKVTPMDSQIMLLGRHLKEFAQAVRPADQNSVARALSPKYDFTFHGERFGR
                     VKKKVLVKIMDDFKRLIRFTQDLALNEKLIYDIHTENIIITLPEFELKIFDFHIFDHH
                     LYAGGGDFDRPEREHVEVLERFLESLSGMGEE"
     gene            complement(1859..3001)
                     /locus_tag="PLG78_14915"
     CDS             complement(1859..3001)
                     /locus_tag="PLG78_14915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE24463.1"
                     /translation="MARLDPSRISVLHLNAARTWRGGEQQTLYLAEGLAKAGIRQAII
                     GRPGSELSSRASDKGIEFIPVAMGSEADFLSAFRIATIAREGSYRILHAHTAHAHSLA
                     ILARLFFRDLRVVVTRRVDFHIRGLSGLKYRTSLVDRFCAISANVRDIMLHDGVPEDR
                     IRLAYSGIDLKRFKKLPSSADLRKEFRIGRELVVGCVGALVDHKDQRTLIRAFGNLPE
                     AGEKIGRGIMPRAVLILVGDGNLKEEYEQIATDLFGVGLKARGHRIVFAGFRADIPAF
                     LSLFDIFAMSSKEEGLGTSVLDAMAAGLPLAVTDGGGLREMADPGKGALVSPARDPER
                     LAENLTALLTQKALRDRFSAHNRRRVKDFSVEEMVKANLAVYRELV"
     gene            complement(2988..>3706)
                     /locus_tag="PLG78_14920"
     CDS             complement(2988..>3706)
                     /locus_tag="PLG78_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNE24464.1"
                     /translation="DLLAAGIHFDVHPAAETDGPTGTCVVLTTPDAERTMLTHLGVSI
                     QLSANDINEDRIKASKMIYIEGYLWAGESTRAACIRAAEMGRKHQVPVAFTFSDSFLV
                     APFRSDFFGFVKDYCSVVFCNAPEVRSFSEMDKLEDAALYAGQIADTVFVTNSEHGAL
                     VVEHGKITEVPGFRVKAIDTNGAGDAFAGGTLFGLCRGYSSAHSARWGNFLASEIVQI
                     PGARLTRDYSSQVRTIIGSP"
BASE COUNT          973 a          966 c          858 g          909 t
ORIGIN      
        1 caagtctttc cattattgac aatgctcata aaaaattcat ctcttctctg gtcggaacac
       61 cgatgcagaa ggagattgaa aagcagacat ttgagctttt tcctgacact acagtcattc
      121 cgaatatgac ccattttgtg cgtctgaaga tcatctatga aaatgcatcc tcattttaca
      181 aacttgcgga aagtgcctgg gattccattt tttcgctttc ctggtgcaga tacaatcctc
      241 ggcccggaaa tgaaatcaac gggcgaagtc atgggacttg cacccacatt cgaagaagca
      301 tacgtaaagg cagtgattgg cgcgggagaa aagattccgg aagctggagg agtatttgta
      361 agcgtaaaca acattgcaaa gcagtctctg gccaccgaaa ttaaaatcct caaagacctg
      421 ggctacgtga tgtatgcaac agaaggcaca gctgagtttc tcaaaacaaa gggtgtggat
      481 gtaaaagtgc tgcacaagtt gcgcgatcag gtctctccga gtgcgctcga tgaaatgatc
      541 aatgggaacc tcaagctcat catcaatatt ccttattcgc agacaaccag agacgatgca
      601 tttttgattc gccaggaagc aagccgtcgc ggcattctct gcatcacgac cgttccaggc
      661 gtgcgtgcat tcgtgcgcgg cctcaagcag atgaagaaca agaaatttac agtcaattca
      721 ctgcaagaga ttcaaaagag aattggttga gtccgggagt tttactcctc tcccatgcca
      781 gacagagact ccagaaagcg ttccagcact tccacatgtt ccctttccgg cctgtcaaaa
      841 tcgccgcctc cggcgtagag atgatgatca aagatatgaa agtcaaagat ctttagctca
      901 aactccggca gagtaatgat gatgttttcc gtgtgaatgt cataaatcag tttttcattc
      961 agagcaaggt cctgagtgaa tcgaatgagg cgtttaaagt catccatgat cttcactaga
     1021 acttttttct tcaccctccc gaagcgctct ccgtggaacg tgaaatcata cttgggagaa
     1081 agagcgcgcg ccacgctgtt ctgatctgcc gggcgcactg cctgcgcgaa ttctttgaga
     1141 tggcggccca gtagcatgat ctggctatcc atgggcgtaa ctttttcaga gaaaccttcc
     1201 gtgacgaaat gctttctgta tctttttccc atgaaaaacc tggttggaag aacaagatcc
     1261 ggcagagctt cgcgtactct ccagtagtga tatctctcca tcccaagcct gtggaatttg
     1321 aaatgaagct tctcttttgt ggatgagcct ttcccctgac acaaatgctc cagcagttcc
     1381 agttcctccg cgctcaagaa tcgctcggga ttcctggcca cctctctata catagacccg
     1441 aagataggat ctgaaggaag aatttcttta gaaactttaa ggaccattct ccagggtaaa
     1501 cgatagacga acttgtagga accccttcca gcaaacgcat ccggactgat gggcatgaaa
     1561 tcttctagaa acggagctcc atacttccag gacatgcgga acacatgatc cacaggaaat
     1621 aatctttcgt aaatgcgcct ggggagactg gattcgcgca ggcgaaaatc agatggaaga
     1681 ctgtccggag acggcgcgag catctgtctg tatctatgaa acgcagactc catccattca
     1741 tcgctgaatg tatcaatgac agaccgcatc ctatcgccgg cttctctgct cttttccgtc
     1801 atgcgatcgc gggcttcgcg cacacgatga ttgactcttc tgaaaatatc agtgaatttc
     1861 aaacaagttc cctgtaaacg gccaggtttg ccttcaccat ctcttctacg ctgaaatcct
     1921 tcactcttct tctgttgtgg gcgctgaatc tgtcgcgaag agctttctgg gtaagaagag
     1981 ctgtcagatt ctccgccagc ctttctggat cacgagcagg agaaacgagt gcgcccttcc
     2041 ctggatctgc catttctctg agtcctccac catctgttac ggcaagagga agtcccgcag
     2101 ccattgcatc gagtacagaa gtacccagcc cttcttcttt ggagctcatg gcaaagatat
     2161 caaagagaga aaggaaagca ggaatgtcgg cgcgaaatcc ggcgaaaaca attctgtgac
     2221 cacgtgcttt gaggcccaca ccgaacagat ccgtggcaat ttgctcgtat tcttctttca
     2281 gatttccatc ccctactaaa atcaatacag cacgcggcat aatcccacga ccaatcttct
     2341 ctccggcctc gggcagattt ccaaacgctc tgatcaaagt tctctggtct ttgtgatcca
     2401 cgagtgcgcc cacacatccg actacaagtt cgcgaccgat tcgaaattcc ttcctcagat
     2461 cagcagaaga cgggagtttc ttgaagcgct tcaaatcgat tcccgagtaa gccagccgga
     2521 ttcgatcttc cggcacacca tcatgaagca tgatatcacg aacgttagcc gaaattgcgc
     2581 aaaaccgatc cacaagagaa gtgcggtact tcaaaccgga gagtccgcga atatgaaaat
     2641 ctacacgtct ggtcacaacg accctgaggt cgcgaaagaa gagcctcgcg agaatggcca
     2701 gactgtgcgc gtgagctgta tgcgcatgca ggatgcgata cgacccttcg cgcgcaatgg
     2761 tggcgatgcg aaatgcggac aggaagtccg cttcacttcc catggccact ggaatgaatt
     2821 caattccttt atcactcgcg cgcgaagaaa gttcactgcc aggtctgcca atgatcgcct
     2881 gacggatgcc tgctttagcc agaccctctg ccaggtataa tgtttgttgt tcaccgccgc
     2941 gccaggtgcg agccgcgttt aaatgtagga cggaaatccg tgacggatca aggcgagcca
     3001 atgatagttc tcacctggct tgagtagtct cgcgtgagtc tcgctccagg aatctgaacg
     3061 atttcacttg ccagaaaatt tccccagcgc gcagaatgag cgctggaata tcccctgcaa
     3121 aggccgaata acgttccgcc agcaaaagcg tcccctgctc cgtttgtatc aatcgctttg
     3181 actctaaagc caggcacttc tgtgattttt ccgtgttcca cgaccagagc accatgttcg
     3241 ctattggtta caaatactgt atctgcgatc tgtcctgcgt acaacgccgc gtcttccagc
     3301 ttgtccattt cgctgaaact tcgaacttcg ggggcgttgc agaatacaac agaacaatag
     3361 tcttttacaa atccaaagaa atcagatcga aaaggagcca caagaaagct gtcagaaaaa
     3421 gtgaaggcaa caggaacttg atgctttctt cccatttccg ccgcgcgaat gcaggctgct
     3481 ctcgtggatt cccctgccca caaatagcct tcgatgtaga tcatcttact tgctttgatt
     3541 ctgtcctcgt tgatgtcatt tgcagacagt tgaatggaca cgcctagatg agtgagcatg
     3601 gttctttctg catccggtgt ggtaagaaca acgcaggttc cagtgggccc atctgtctca
     3661 gccgccggat gcacatcaaa gtgaatcccg gctgccagca aatcct
//
LOCUS       DAOQJL010000396         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15555_length_3706_cov_3.056697, whole genome shotgun sequence.
ACCESSION   DAOQJL010000396 DAOQJL010000000
VERSION     DAOQJL010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15555_length_3706_cov_3.056697"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..274)
                     /locus_tag="PLG78_14925"
     CDS             complement(20..274)
                     /locus_tag="PLG78_14925"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNE24465.1"
                     /translation="MMDRSHELFVRFVDQMASGRFYEAHESMERLWALCGYEPASPYR
                     GLTQLAVALAHLEKENFEGAQSVLAKARKILFENEAVHVL"
     gene            complement(271..1245)
                     /locus_tag="PLG78_14930"
     CDS             complement(271..1245)
                     /locus_tag="PLG78_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24466.1"
                     /translation="MTLEEKRKIKQAAATFVVLFVLFNILISARILTHRLPQNQWQLN
                     TIRAETYVFKKNTWKYVVLGSSLTFRIAPGVDREDAYVMAFAGDSAFTGLEIIRRSGK
                     VPEYIFVEVNTLGMQPDEGKIQPLFSLQGKLRRAVHAFREENTPLNFIMYPVARAILG
                     ISEGISNLAGRIAPSYKEEEKKWSTYDPFRGAVPPENKKAGEPIPQKVESEPVIVPEN
                     LRSTAETKKVEEALIRLQGAVRDVESRGAKVVFFEMPMLPSRRTDSYKMLKLKLDELF
                     PPDHYKRVPQADDRNLVYSDGLHLDMPSSLSYAKFLMGFFPDAKPPSR"
     gene            complement(1257..2672)
                     /locus_tag="PLG78_14935"
     CDS             complement(1257..2672)
                     /locus_tag="PLG78_14935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE24467.1"
                     /translation="MLFNSYSFLLFLGVTFLLYYTPFLRKYQRQLLVFASFFFYSFEK
                     PVLLFLLLASILINSICSYWIIHARDSYRKATLAIGLIANLSLLAFFKYAPLISSTFS
                     AFGSSNDFHDFIAGIPLPLGISFYTFQGISLLVDCYRMHEAGTDEVIRIDSFPSHLLH
                     TTLFISFFPHQVAGPIVKAHLFFPQIKNKSLKDIEIYAAFRTIVTGYFLKTVIADNLK
                     DQTFWLDYPYFQYFSTTILFTLLFGFSIQIFSDFAGYTLIAIGIARLFGYELTRNFLF
                     PYIAISFSDFWKRWHISLSSWLRDYLYIPLGGNRANLLRTCINMMIVMILGGLWHGAA
                     WGYAIWGAFHGILLVAERLLARFIKLPESLFVTGLRILFVFVMVSFAWTLFRIHEWQH
                     VVLFISSVKNNIHIPTDYAAIAAVLLLSLPVFVYHIMYLVRENPSGILIKKYEGILYG
                     CMIFFIIFNAGNPGKFIYFQF"
     gene            2867..>3706
                     /locus_tag="PLG78_14940"
     CDS             2867..>3706
                     /locus_tag="PLG78_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24468.1"
                     /translation="MRGANLARIRTRELSTILRKKMKHSIPGLILIVCLSTLLARPTN
                     VPFFAGNGQMFVKVGAMGEVSVWGHHSSLGPGDGTRATNADSPVRLGVPRALSAAAGN
                     SHVLILLADGTIIGWGTNAECEVGNGNPGKPLKRWERLEEVRKPVPVTGITGVRMIAA
                     SDKISGAVREDGTVWIWGSGNNGLGGNGIMEPQGSTVACIPVPRKVEGLGQIRSLSLS
                     QTHAIAVGTDGSVYTWGMNGYGQLGDGTSTHRARPARVEGIEDALDAFAGGTMSAVVL
                     KDGS"
BASE COUNT         1041 a          870 c          939 g          856 t
ORIGIN      
        1 tttgatgaga tccacatatt taaaggacat gtactgcttc attttcaaat agaatctttc
       61 tagctttcgc aagaacgctc tgcgctcctt caaagttttc cttctccaga tgagccagtg
      121 cgacagcaag ttgagtcagc ccgcgatatg gacttgcagg ctcataacca cacagcgccc
      181 agagtctttc catggattcg tgcgcttcat agaatcgccc ggaggccatc tgatctacga
      241 accgtacaaa tagctcatgc gaacgatcca tcatctggat ggaggcttag cgtccggaaa
      301 aaaccccatc agaaatttag catagctcaa agaactcggc atatccagat gcaatccatc
      361 tgaatatact agatttctgt catctgcctg cggaacccgt ttgtagtgat ccgggggaaa
      421 caattcgtcc agttttagct tgagcatttt gtacgaatcg gttctgcgcg acggaagcat
      481 gggcatttca aagaaaacaa ctttcgctcc gcgtgattca acgtctctca ctgctccctg
      541 aaggcgaatg agagcttctt ctaccttctt cgtctccgcc gtgctgcgca aattctcagg
      601 cacaatgaca ggctcagatt ctaccttctg cggaattggt tcacctgctt tcttgttctc
      661 cggcggaacg gcaccgcgaa aaggatcgta ggtagaccat ttcttctctt cctctttgta
      721 ggaaggggca attcgtcccg caaggtttga aatgccctca ctgattccca gaatcgctcg
      781 cgccaccggg tacatgataa aattgagagg agtgttctct tctctaaaag cgtgcacggc
      841 tctgcgaagc tttccctgaa gagaaaaaag aggttgtatt ttgccttcat ctggttgcat
      901 gccgagcgta ttgacttcaa cgaatatata ctccggtacc tttccgcttc tgcgaatgat
      961 ttcaagcccc gtgaacgcgg aatccccggc gaacgccatg acgtacgcgt cctcacgatc
     1021 caccccgggc gcaatgcgaa acgtcaaaga tgacccgagc actacatact tccaggtatt
     1081 cttcttgaaa acataagttt ccgcgcgaat ggtattgagc tgccattggt tctgcggcaa
     1141 tctatgagtg aggatgcgcg cggagatcag gatgttaaaa agcacgaaca gcaccacgaa
     1201 cgttgccgca gcctgtttta ttttgcgttt ttcttctaaa gtcatcctta aacctcctaa
     1261 aactgaaagt agatgaactt tccgggattc ccggcgttaa agataataaa gaagatcatg
     1321 caaccgtaaa ggatcccttc atatttcttg atcaaaatgc ccgatggatt ctcgcgaacc
     1381 aggtacatga tgtgatacac aaaaacaggg agcgaaagca aaagcacggc agcgatcgct
     1441 gcgtaatccg ttgggatgtg tatgttgttt tttaccgagg agatgaatag aactacgtgt
     1501 tgccattcgt gaattctaaa caacgtccac gcgaacgata ccatcacaaa gacgaacaaa
     1561 attcgtagcc ccgtcacaaa caatgattca ggaagtttaa taaaccgcgc aaggagcctc
     1621 tccgctacca gcaggattcc atggaatgct ccccagattg catagcccca tgcagccccg
     1681 tgccacagtc caccgaggat catcacaatc atcatattga tgcatgtccg cagtaggttc
     1741 gctcgatttc cgccgagtgg aatgtagaga tagtcacgaa gccagcttga aagcgaaata
     1801 tgccagcgct tccagaaatc ggagaatgaa atagcgatgt aaggaaacag gaagttgcgc
     1861 gtaagttcat atccgaagag acgcgcgata ccaatggcga tcaaagtata gccggcgaaa
     1921 tcggaaaaaa tctgtatgga aaaaccaaac aacagagtaa acagaatggt ggttgaaaaa
     1981 tactgaaaat acggatagtc gagccagaat gtttgatcct tcagattatc tgctatgact
     2041 gtcttgagaa agtacccggt cactatggta cggaacgccg catagatttc aatgtccttc
     2101 agtgatttgt ttttgatttg cgggaaaaac aaatgcgcct tcacaatcgg tccagccact
     2161 tgatgcggaa aaaaagaaat gaacagcgtg gtgtgcaaga gatgcgaagg aaaactatcg
     2221 attcgaatca cctcatctgt acccgcttca tgcatgcgat aacaatctac cagcaggcta
     2281 attccctgga aagtgtagaa tgaaattccc aggggcaagg gaatgcctgc gatgaagtcg
     2341 tgaaaatcat tggaactgcc aaacgcggaa aaagtggagc tgatcaaagg tgcgtacttg
     2401 aagaaagcaa gcagcgacag gtttgcaatc aatccaatgg caagcgttgc cttgcgataa
     2461 gagtctcgcg catgaatgat ccagtagcta catatagaat tgatcaaaat ggacgcgagg
     2521 agtaagaaca gaagcactgg cttttcaaat gagtaaaaaa agaaactggc gaaaacaaga
     2581 agctgtctct ggtatttgcg gaggaagggc gtgtagtaga gaagaaacgt tacaccaaga
     2641 aaaaggagaa agctgtagct attgaagagc attgattgga gaggctagcc tcactcgcaa
     2701 agcatgccag tcaagcaaaa ggaattggct gcattcgaga atcactcgca ggacacgaaa
     2761 tatttgctgg atgcttgttc ttttccatag tcaaaaaaat gcctgccttg tcttaacgag
     2821 agaaggggcg aattgccggc tgcccgggct tgccgtaaag caggccgtgc gaggcgcaaa
     2881 tctggcccgg atccgtactc gcgaactatc tacaatactg aggaaaaaga tgaagcattc
     2941 tatcccgggg ctgatcctaa tcgtctgttt atccactctg ctggcaaggc cgactaacgt
     3001 tccttttttt gcgggcaacg ggcagatgtt tgtaaaagtg ggagccatgg gtgaggtaag
     3061 cgtctgggga catcattcgt ctctgggacc gggagacggc acccgagcaa caaatgcgga
     3121 ttctcctgtt cgacttggtg tgccgcgcgc cttatctgct gcagccggaa attcacatgt
     3181 tttgattttg ctcgcagatg gaaccatcat cggctgggga acgaacgcag aatgcgaagt
     3241 gggaaacgga aatccgggca aaccactgaa gagatgggag cgcctggaag aagtgcgaaa
     3301 gcctgtgcca gtgacaggaa tcaccggagt caggatgatt gccgcttccg acaaaatatc
     3361 tggagcagta cgcgaagacg gcaccgtttg gatctggggc tcaggtaaca atggtctggg
     3421 tggcaacggc atcatggaac cacaggggtc cactgtggcc tgcattcctg ttccgcgaaa
     3481 agtggaaggc ctgggtcaga ttcgctctct ttctctttcg cagactcacg cgattgcagt
     3541 gggtacagac ggaagcgttt acacatgggg aatgaacggt tacggacagt taggcgacgg
     3601 aacatccaca catcgtgcga ggccggctcg cgtggaaggc attgaagacg cgttagacgc
     3661 ctttgcgggc ggtacaatgt ccgcagtcgt gttgaaagac ggaagc
//
LOCUS       DAOQJL010000397         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15567_length_3703_cov_3.832785, whole genome shotgun sequence.
ACCESSION   DAOQJL010000397 DAOQJL010000000
VERSION     DAOQJL010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15567_length_3703_cov_3.832785"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..933)
                     /locus_tag="PLG78_14945"
     CDS             complement(148..933)
                     /locus_tag="PLG78_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24469.1"
                     /translation="MRFIALILISVTGFCRQSYAGFVAETLSSDSMLGRESGTPSAKK
                     TADWIRDQYIAMGAYPAFASGYTQEFSFKAGVEPEKSTLAIGTRTLDGIPLPLSAPGS
                     AEGKFAFGGYCVESDEHNVHDLATLDLQNRIVLCLRWTSEGEKDRKLARAMSFQSKWH
                     NLAKRKAAGVIFLYPEGSEPPAPGDFMAGHEAGPIAISLPTKALTELFPWLASAQDKA
                     QKGDVCAAGNECKGTIQIAYTSKQKTGYNVGAFLRQRAPLAQQ"
     gene            complement(980..2308)
                     /locus_tag="PLG78_14950"
     CDS             complement(980..2308)
                     /locus_tag="PLG78_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE24470.1"
                     /translation="MGNVLVLVRPPAKVAMVDVRKNKLLLIQSLLLDLIGFSLIFPLV
                     PHLLEYYLNAAANTPMDSWLPPAADYVRGLLPEDRRSSAELIVLIGGILASIYSFLQF
                     SVAPFWGRLSDRIGRRPVLIMTSCGLAASYLLWFFSTSFTMFLLSRVLGGAMAGNMGV
                     VSASMADMTEPKDRTRAMGMLGATFGIGFILGPVIGGLSSLANLPQMFPGLHWLNPYS
                     SCALVSVLMSIGSATVNSALFTETLLHPVARKESVEIPTSDLRGLPAVLLIGFLFTFL
                     FAAFEFSLTFFYKAEFSLGPAAMGFIFSYLGILIALGQGGLVRRLSGKVQERSMAFVG
                     LSLLPVPLALLAHAPGVGVSLLILIPLSIGASMIRPSLSSLASLLAPGHKQGQSLGVF
                     RSAESLGRALGPICGAYLYWVYGVKFSYATLAALLAVTSLLCLLIRKADA"
     gene            2353..3180
                     /locus_tag="PLG78_14955"
     CDS             2353..3180
                     /locus_tag="PLG78_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24471.1"
                     /translation="MAGSEPIPIADLTAKDHVVSVGKDYIPTIERKLRATIMNIFRDP
                     SGQQGNEISNHELLSEAVFWSLGELINNANKANNRWALLRNALMQRIVAENKLQNDQA
                     AQDKVQTEVDYAIEHNQTDLLKKYNLTNVDLTSSILKLIEMHKTNSFALSERFNKKID
                     VTLRVKAKSGHQTLFVNVINNSPITVIDKQRVEYNLEKIKEDLMMAGKNSRDASVRLY
                     DKQEDHAGGGFGAGLRSIILFLKEGYAPFTAEIKYSTLIQYRSAGNSTIFSVELPIP"
     gene            3273..>3703
                     /locus_tag="PLG78_14960"
     CDS             3273..>3703
                     /locus_tag="PLG78_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNE24472.1"
                     /translation="MREIKTVTVLGANGTMGANSAAIVAAFGHAKVHMLARSVDKAKE
                     GIKIAVDSVRSGVIEKNFIPGTYDADLEKAVSESDWVFELVAEDYAIKEPLNKRIAAS
                     RKPGTIVSTVSSGLSIERLASHFDADGQKYYYGTHFFNPPY"
BASE COUNT         1011 a          903 c          957 g          832 t
ORIGIN      
        1 tgctagattt tgagttaatt ggcttgagta gtcgtgacag aagagtttat gaagcacttc
       61 tgactgtgcc acgctcgagc gtgcgcaaag tagcggaaga aacagggata aatagaggga
      121 gcgtatttga gtctataaaa gcgctggtta ctgttgggct agtggcgcac gttgccgaag
      181 gaaggcgccg acattatacc ctgttttctg tttgctcgtg tatgcgattt gaatggttcc
      241 tttgcattca tttccagccg cgcatacatc tcctttctga gctttgtcct gcgcagaggc
      301 aagccacgga aataattctg tgagagcttt tgtaggaagt gagatcgcaa tcggtcctgc
      361 ttcatgtccg gccataaaat cgccgggggc gggcggttcg cttccttctg gatagagaaa
      421 aataactccg gcggctttgc gttttgccag gttgtgccat ttgctctgaa agctcatggc
      481 gcgcgcgagc ttgcgatctt tttcaccctc ggaagtccat ctcaggcaaa gcacaatcct
      541 attctgcaaa tcgagcgttg ccagatcatg cacattgtgt tcgtctgatt caacgcagta
      601 tccgccaaat gcgaatttac cttctgcaga tcctggtgcg gacaggggca atggaatgcc
      661 gtctaacgta cgggttccga tcgccagggt gcttttttcc ggttccactc ctgctttgaa
      721 tgaaaactct tgcgtataac cagatgcgaa ggcaggatat gcccccatgg caatgtactg
      781 atcgcgaatc cagtctgctg tttttttggc ggagggtgtt cctgattctc ttccgagcat
      841 ggaatccgag ctgagtgttt ctgccacaaa gcccgcgtaa ctctgcctgc aaaagcctgt
      901 tacagatata aggatgagtg caatgaatcg catgcttaca tggtacggtt ggactggccc
      961 gccggcaagt gcaatcagct catgcgtctg ctttgcggat caagagacag agaagcgaag
     1021 taacagccag aagcgccgcc agtgtggcgt aagagaattt cactccgtag acccagtaga
     1081 ggtaggctcc acagataggg ccaagtgctc ttcccagaga ttctgcggaa cgaaacactc
     1141 cgaggctctg cccctgtttg tggcctggtg caaggaggct tgcgagactc gaaagcgaag
     1201 ggcgaatcat ggacgcgcca atcgacaatg gaatgagaat caaaagagat acgccaacac
     1261 ctggcgcatg agcgagaagc gccagaggca caggaagaag agacaatcca acaaaagcca
     1321 tagatctttc ctggactttc cctgatagcc ttcgcacaag tccaccctgg cccagggcga
     1381 tcaggattcc cagataggaa aaaataaatc ccatggccgc cgggccgaga gagaattctg
     1441 ctttgtaaaa aaacgtaaga gaaaactcaa aagcagcaaa aaggaatgtg aacaagaagc
     1501 cgatcaagag cactgcgggc aggccacgca aatcagacgt tggtatttcc acagattctt
     1561 ttcgcgctac aggatggaga agcgtctccg taaacagagc agagttcaca gtggcagatc
     1621 caatcgacat gagtacggat acaagagcgc aggatgaata cggattgagc cagtgaaggc
     1681 ccggaaacat ttgcggtaga ttggcaagag aagaaagtcc gccgatgacc gggcccagga
     1741 tgaatccgat tccaaacgtc gcacccagca ttcccatcgc tctggttctg tcttttggtt
     1801 ctgtcatgtc cgccatggac gcagagacca ctcccatgtt tccagccatc gcacctccca
     1861 ggacacggga gaggagaaac atggtgaagc tggtggagaa aaaccagagc aaataagacg
     1921 cggccagccc gcaactcgtc atgatcagaa caggcctgcg gccgattctg tctgaaagtc
     1981 taccccagaa aggagcgact gagaattgaa ggaaagaata gatggaagca aggattccgc
     2041 caatcaagac gatgagttct gcgcttgatc gcctgtcttc tggcaaaagt ccgcgcacat
     2101 agtccgccgc tggaggaagc cagctgtcca tgggtgtatt tgcggccgca ttcagataat
     2161 attctagaag atgcggaacc agtggaaaga tgagggaaaa accgatcaaa tccagcagta
     2221 gactctggat caagagtagc ttgttcttgc ggacatccac catggcgacc tttgccggcg
     2281 gccttaccag aaccagaaca tttcccaaaa aatgccttta tctatccatg ccggatggaa
     2341 atgtagcgcc ctatggctgg ttctgagccg attccaatag cggacctcac cgcaaaagat
     2401 catgttgtgt ccgtcgggaa ggattatatc ccgaccatcg agcgcaagct tcgcgctacc
     2461 atcatgaaca tctttcgcga cccttccggg caacagggaa acgaaatctc gaaccatgaa
     2521 cttctctctg aagcggtctt ctggtctctg ggggaattga tcaacaacgc gaacaaggca
     2581 aataatcgct gggcgctgct tcgtaacgcg ctcatgcaaa gaattgtcgc ggaaaataaa
     2641 cttcagaatg atcaggcagc ccaggacaaa gtgcagacag aagtagatta tgcaatagag
     2701 cacaaccaga ctgacttact aaagaaatac aaccttacaa atgtggacct gacctcttct
     2761 attctcaaac tcattgagat gcacaaaacc aattcattcg ctctttcaga aagatttaat
     2821 aagaagatag atgtgactct tcgcgtgaag gcaaaaagcg gacaccagac cctgtttgta
     2881 aacgtaatca acaattcgcc tatcacggtc attgacaagc agcgtgtgga atacaacctc
     2941 gagaagatca aagaagacct catgatggcg ggtaagaatt cgcgcgacgc atctgttcgc
     3001 ctttacgaca agcaggaaga tcatgcgggc ggcggatttg gcgcgggtct gcgaagcatc
     3061 atccttttcc tcaaggaggg atatgctcct tttacggctg agatcaagta ttctactttg
     3121 atccagtatc gttccgcggg caattccacc atcttctccg tagaattgcc cataccctga
     3181 tccggggtga ttcgtgcaaa atgcgatcgt tcttgaccgc ctacgcgttt agacggattc
     3241 aggattattc gaaatagacc ctggagtttg gaatgagaga gatcaagact gtaaccgttt
     3301 taggcgcaaa cggaacgatg ggcgcaaata gcgcggcgat tgtggccgcc tttgggcatg
     3361 cgaaagtcca tatgctcgcg cgcagcgtag acaaagcaaa agaaggcatc aagatcgcag
     3421 tagactctgt tcgctccggt gtgatcgaaa agaatttcat tcctggaacg tatgatgcgg
     3481 acctagaaaa ggcagtttct gaaagcgatt gggtgtttga gcttgtagca gaagactacg
     3541 cgatcaaaga acctctcaat aagagaatcg cagcatcacg taagccaggc accattgtat
     3601 ctactgtcag ctccggtctt tccattgaga ggctcgcatc tcattttgat gcagacgggc
     3661 agaagtacta ttacggcact cacttcttca atcctcccta caa
//
LOCUS       DAOQJL010000398         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15682_length_3688_cov_3.611616, whole genome shotgun sequence.
ACCESSION   DAOQJL010000398 DAOQJL010000000
VERSION     DAOQJL010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15682_length_3688_cov_3.611616"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLG78_14965"
     CDS             complement(<1..573)
                     /locus_tag="PLG78_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE24473.1"
                     /translation="MSSFSRIPYGRQTVTQEDIDAVVETLRSDFLTQGPKIAEFEEAF
                     AAYVGAKYAVAVANGTAALHLAALALGVGGKSRVLTTPITFVASANCVLYCGGSVDFV
                     DVDPESALLDLDALEIKLKKSPGLYAGVIPVDFAGLPVDLEKLRSIADRHGLWILEDA
                     CHAPGASFRSKAGTWEKCGSGKWADISIFSF"
     gene            complement(570..1577)
                     /gene="pseB"
                     /locus_tag="PLG78_14970"
     CDS             complement(570..1577)
                     /gene="pseB"
                     /locus_tag="PLG78_14970"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNE24474.1"
                     /translation="MLNQKSILVTGGTGSFGKRFVGTILKQFPDIKRLVVYSRDELKQ
                     FEMAQEFPESQYKQIRYFIGDVRDKERLSYAVEGIDTVVHAAALKQVPAAEYNPLEAI
                     KTNILGAQNLIEACIDKGVKQVVALSTDKAAAPINLYGATKLCSDKLFIAANLYRGNH
                     DIKFSVVRYGNVMGSRGSVIPFFLKQKEKGFLPITHPGMTRFNITLDEGVNMVLFALA
                     NMWGGEIFVPRIPSYRILDVAEAIAPGMEKRIVGIRPGEKIHEEMITETDAISTIEFE
                     KHFVILPSFQPAWNIDEFRSHFKGSFCKEGFRYNSGENSEYLSVDQLRTLIQSAAIKY
                     E"
     gene            complement(1612..2259)
                     /gene="hisH"
                     /locus_tag="PLG78_14975"
     CDS             complement(1612..2259)
                     /gene="hisH"
                     /locus_tag="PLG78_14975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNE24475.1"
                     /translation="MSFDVVVIDYGIGNLYSVRRAFEACGANVVLTDDPALIVSAPRL
                     VLPGVGAFADGMRGLIERGLDSVVREYAKSGKPLVGICLGMQMLATVSMEFGEHRGLN
                     LIPGKVVRIPSTTMEGAPHKIPHIAWEALVRPDFIETWDNTILANTRPGATMYHVHSF
                     TVVPDDDAHRLADCYYNGRQISSVIRMGNVYGCQFHPEKSGRAGLALLKRFLEMS"
     gene            complement(2249..3016)
                     /locus_tag="PLG78_14980"
     CDS             complement(2249..3016)
                     /locus_tag="PLG78_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNE24476.1"
                     /translation="MSTIRLIGRLDIKGPNLIKGIHLEGLRVIGDPQEFARKYYEQGI
                     DELVYMDIVASLYGRNSLHDIIRSAAHDVFVPLTVGGGIRSVDDAREILRAGADKVAI
                     NTAAVKRPDLIREIAHVFGSQCMVLSIEAKCQAKDRWEVYTDNGRERTGLDVVEWAKR
                     AVGLGAGEILLTSVDQEGTRKGFDCDLVRAVSDSVSVPVIASGGMGTVEHLKQVVTDG
                     HADAVAMADILHYNRSTLAEIRSSAAALGVSVRSDEL"
     gene            complement(3041..>3688)
                     /locus_tag="PLG78_14985"
     CDS             complement(3041..>3688)
                     /locus_tag="PLG78_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNE24477.1"
                     /translation="GIQLIMYGENGEVEYGGDMKNAHRPTRDIQDHDTHYFSGMPPEF
                     WTEHGVSLADLKPFAAPPYESITKNKTEIHFFSYYKFWDPQENYYYCREHTGFTPNPE
                     RSQGTYSKYASLDDKIDGFHYYLAYIKFGIGRATSDAAHEIRDGKITREEGVALVKRY
                     DGEFPHKYFDEFLDYCNITEAEVHEVIDSWRSPHLWEQKDGVWQLKYASYLDTPV"
BASE COUNT          921 a          985 c          867 g          915 t
ORIGIN      
        1 aaaagaaaag atagaaatat ctgcccattt gccgctgccg cacttctccc aggttcccgc
       61 cttacttcga aaagacgccc cgggggcatg acaggcatcc tctagaatcc acagaccatg
      121 cctgtctgcg atcgaacgaa gcttctcaag atccacgggc aatcctgcga agtccacagg
      181 aatgacaccg gcatacagcc ccggactctt ttttagtttt atttcaagag cgtctagatc
      241 aagaagcgcg ctttcaggat ccacatctac aaaatcaacg ctccctccgc agtagagtac
      301 acagtttgcc gatgccacaa acgtgatggg cgttgtcagc acacggcttt tgccgccgac
      361 tccaagagca agagcagcaa gatgcagagc agcagtacca tttgctactg ccaccgcata
      421 cttcgcacca acgtaggccg caaacgcttc ctcaaattcg gcaatctttg gcccctgagt
      481 gagaaagtct gaacgcagtg tctctacgac tgcgtctatg tcttcctgcg taacagtctg
      541 cctgccgtaa ggaattcgag aaaaagaact cattcgtatt tgatggcggc gctctgtatc
      601 agtgtgcgca gctggtcgac ggaaaggtat tctgagtttt cgccgctgtt gtagcgaaat
      661 ccttctttgc aaaagctgcc tttgaaatgg ctgcgaaact cgtcgatatt ccaggcgggc
      721 tgaaaagaag gaagaatgac aaagtgtttt tcaaattcga tagtgctgat cgcatccgtc
      781 tccgtaatca tttcctcgtg aatcttttcc cctgggcgga tgcccacaat gcgcttctcc
      841 atgcctgggg cgattgcctc tgcaacgtcg agaatacgat agctgggaat gcgtggcacg
      901 aaaatctctc cgccccacat gttggcaagg gcgaacaaca ccatattcac gccttcatcc
      961 agagtgatgt tgaagcgagt catgcctgga tgagtgatgg gcaagaaccc cttttctttt
     1021 tgtttgagaa agaacggaat cacagagccc cggcttccca tcacatttcc atagcgtaca
     1081 acagagaact tgatatcgtg atttccgcga taaagatttg ctgcgatgaa aagcttgtct
     1141 gaacagagtt tggttgctcc gtaaaggttg atgggcgcgg cagctttgtc tgtggacaat
     1201 gcaacaacct gtttaacgcc tttgtcaatg catgcttcaa tcagattctg cgcgcccagg
     1261 atgtttgtct tgattgcttc gagaggattg tattctgccg cgggaacctg cttgagggcc
     1321 gccgcgtgca ccaccgtgtc aattccttct accgcatacg aaagacgctc tttgtcacga
     1381 acatcaccaa tgaaatagcg aatctgcttg tactgcgatt caggaaattc ctgcgccatc
     1441 tcaaactgtt tcagttcatc acgcgaatag accacgagac gcttgatatc ggggaactgc
     1501 ttgaggatag ttcccacgaa tcgctttcca aaggagccag tgcccccggt tacaagaatg
     1561 gatttctgat tcagcacaat cttacttctt tttaataaat tttcttaacc actaagacat
     1621 ttcaagaaaa cgcttcaaaa gtgcaaggcc tgcacggccg cttttttcag gatgaaactg
     1681 gcagccatac acattgccca tgcggatcac agaagaaatc tggcgcccgt tgtagtagca
     1741 atctgcgagt ctgtgcgcgt catcatccgg aaccacagtg aatgaatgca cgtgatacat
     1801 ggttgcacct ggtctggtat tcgcaagaat tgtgttgtcc cacgtttcaa tgaaatcagg
     1861 gcgcaccaga gcttcccagg ctatgtgagg aatcttgtga ggcgctcctt ccatggtggt
     1921 agaaggaatt cgaaccactt tgcctggaat cagattgagt ccgcgatgtt ctccaaattc
     1981 catgctgacg gttgcaagca tctgcattcc cagacaaatt cccacgagag gctttcctga
     2041 tttcgcatat tcgcgaacaa cagaatcaag tcctcgctca atgaggcctc gcatgccgtc
     2101 tgcgaaagcc cccactccag gcagtaccag tctgggagcg gatacgatga gtgcgggatc
     2161 atctgtaaga actacatttg ctccgcatgc ctcaaaagcg cgccgaacgc tgtagagatt
     2221 cccgatcccg taatcgatta ctactacgtc aaagctcatc cgatctcacc gaaactccga
     2281 gtgcggccgc agatgagcga atctccgcga gagtagaacg attgtagtgt aaaatatcag
     2341 ccatcgcaac cgcgtccgcg tgcccatctg taaccacctg cttgaggtgt tcgactgtac
     2401 ccatcccacc gcttgcaatg accgggacag aaaccgaatc actcactgcg cgaacgagat
     2461 cgcaatcaaa gccctttcgc gttccttctt gatcgacaga agtaagaaga atttcgcctg
     2521 cacccagacc gacagcacgc tttgcccatt ccaccacatc caggcctgtt cgctcgcggc
     2581 cattgtctgt gtaaacttcc cagcgatctt ttgcctggca cttcgcttca atagaaagaa
     2641 ccatgcactg ggatccaaat acgtgggcaa tttcccgaat caaatcagga cgcttcaccg
     2701 cggcagtatt gatggcgact ttgtctgcac ctgcgcgcaa aatttctcgc gcatcatcta
     2761 cagaacgaat tccacctccc acagtgagag gaacaaaaac gtcgtgcgca gcgctgcgaa
     2821 tgatatcgtg cagactgttt ctaccgtaga gacttgcaac aatatccatg taaacgagct
     2881 catcgatgcc ctgttcgtaa tacttgcggg caaactcctg agggtccccg atgactcgaa
     2941 ggccttcgag atgaatgcct ttgatcaggt tcggtccctt tatgtcgaga cggccgatga
     3001 gtcgaatggt gctcatggat gaattttgta cgggcgtgac ttagaccggc gtatcaagat
     3061 aagaagcata tttgagctgc cacacaccat ctttctgttc ccacaggtgg ggcgatcgcc
     3121 agctgtcgat cacttcatga acctccgctt ccgtgatgtt gcagtagtcc aaaaactcat
     3181 cgaaatattt gtgaggaaac tcaccgtcgt atcgcttaac gagtgctaca ccctcttcgc
     3241 gggtaatttt gccgtcacgg atttcatgcg ccgcatccga ggtcgcgcgt ccaattccaa
     3301 atttgatata cgccaggtaa tagtgaaagc cgtctatctt gtcatccaga ctcgcgtact
     3361 ttgagtaagt gccctgagaa cgttctggat tcggagtgaa gcctgtatgc tcgcggcagt
     3421 agtagtaatt ctcctgagga tcccagaact tgtaataact aaagaaatga atctcggtct
     3481 tattcttcgt aatcgactcg tacgggggtg cggcaaacgg ttttaaatcg gcaagactca
     3541 ctccatgctc cgtccagaat tctggcggca tgccggaaaa ataatgagtg tcatgatcct
     3601 gaatatcgcg tgttggcctg tgagcgtttt tcatatcgcc gccgtattct acttcgccgt
     3661 tttctccata catgatcagc tgaatgcc
//
LOCUS       DAOQJL010000399         3678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15758_length_3678_cov_4.317140, whole genome shotgun sequence.
ACCESSION   DAOQJL010000399 DAOQJL010000000
VERSION     DAOQJL010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15758_length_3678_cov_4.317140"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1421
                     /gene="alr"
                     /locus_tag="PLG78_14990"
     CDS             282..1421
                     /gene="alr"
                     /locus_tag="PLG78_14990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE24478.1"
                     /translation="MHTYVEIRASALKSNLIHFRRATSTGVMAVLKSNAYGHDLELCT
                     RAVRGQADWIGVNSIEEAIRVRKVDDSTPVLVMGANDKADYKEIASYASKPVLVLSTI
                     DAIRELEDHLPGHPFHLKVDTGMSRLGHHGVSLQKIFSYLSAHPELNWCGLMTHFANV
                     EDVTDQTYAQEQLNRFDEASAFAVSAAGNRKLAFHTAASGAALLLPESRRDLVRVGIS
                     LYGMWPSRETRISASALTAQGTLDSSFKLEPALQWKTRIVHINAITAGTAVGYGCTYR
                     PVQDSNIAVLPVGYFEGYDRRLSNKAHVLISGKRAPVIGRVCMNMIMVDITHIADAKV
                     GSVATLIGTDEKESVTADDLADWMGTINYEVTTKIEPGIPRILVD"
     gene            1553..2932
                     /locus_tag="PLG78_14995"
     CDS             1553..2932
                     /locus_tag="PLG78_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HNE24479.1"
                     /translation="MKYLASLMIVLAAANCGGGKKKAAMWWMALLGNQTAQTAETTSS
                     AGDSVTTTNNDQTGTTGFSFQTTTTVPVSVTVADSNGPVQGAVVQVQDPSAANTVIFQ
                     AVTNETGQASGTFTVDNTVTEVTLVVDAGGNVVTQPVNTTNLVTCDRDVDYPGDVTIS
                     SGGDQDGDGITDANDAYPSDPTRATKVTIPGSGQSIIAYEDLYPSPGDADFNDYVVKV
                     SNEEDLNAAGKVVRLRGSYTHIARGAGYTHQLKLTLPAAAGALNYTVKQYSAAGALES
                     TTTNHYSNGSGLAILGNSNNTITQSNTSSGQTFTPGKRADIEITFDTPVSQSTLGSAP
                     FDLYIHVNNTGLDVHFPRMYFDANGKDQYMDTNGFPWALQIPEAWKWPLESDNIENGY
                     PGFRPWYLSSGTTNKDWYSSAVSSHVFNASSLFAYLKSPASWMILGLIAAIAVIGLAL
                     AMIQRRRAA"
     gene            2966..>3678
                     /locus_tag="PLG78_15000"
     CDS             2966..>3678
                     /locus_tag="PLG78_15000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE24480.1"
                     /translation="MEYAARIILSTIPVGLFFALYFRNFVFQRSILWQSKAFFLGMLS
                     SIAAIVIQALLPETASPAVRSFFHAALVEEAIRFTVLYLRIRKSSEGFTVTEGIFDGI
                     LVALGFAFSENLHYSATTSGYVILLRCISSVPLHVFCGGIMAYFLSYRSLCRENRKVW
                     RRLNWYGLRRFELAVFALLIPFLLHGFFDYALFRGDVWNYMLVPILVTGFIVTEYMAA
                     RGMQIFGKNILGALGLDAD"
BASE COUNT          911 a          913 c          918 g          936 t
ORIGIN      
        1 catgagccac ctatgagacg aaggaacgcg tccttttcat tgcatcggcc gaaaattcac
       61 gaccaacgat caggattctg atttttcttc gatttccttg aatccggtgc tcatccggtg
      121 ggttctctta gctttgctat ggagggcgcg ctttgtacgc ttttcagcga ctttgatccg
      181 aacggaccgg gtagttttga gctttttcat tttatttagc cgacgagcgg aatttctggc
      241 tagattccat tgggcaccca ggcgaacaag catttaaatt catgcatacg tacgtagaaa
      301 tacgcgcatc tgcgctaaaa tcaaatctaa tccattttcg ccgcgctaca tcgacgggag
      361 tcatggccgt cttgaagtcc aatgcatatg gtcacgatct ggagctctgc acacgggctg
      421 tccggggcca agcagactgg attggagtga attcaataga agaagccata cgcgttcgga
      481 aggtggatga ctcaacgccc gttctcgtca tgggcgcaaa cgacaaggcg gattacaaag
      541 aaattgcgag ctacgcgagc aaacctgttc tggttctttc aacgatagac gccattcgcg
      601 aactagagga tcaccttcct ggacatcctt ttcatttgaa agtagacact ggcatgtctc
      661 gcctggggca ccacggcgtt tctcttcaga agatcttctc ttacctgtcc gcgcatcctg
      721 aattgaactg gtgcggattg atgactcatt ttgcaaacgt cgaggatgta acagaccaga
      781 cgtacgcgca ggaacagtta aatcgattcg atgaagcgtc cgcatttgcg gtgtctgccg
      841 caggaaatcg aaagcttgcg tttcatacgg cagccagtgg cgccgctctt ctcttgcctg
      901 aatccagacg agatcttgtg cgagtcggga tttccctcta cggaatgtgg ccttcgcgag
      961 aaacgcgcat ctctgcgtcc gctctcactg cccagggaac cctggatagt tcgtttaagc
     1021 tggaacccgc tcttcaatgg aaaacaagga tcgttcacat aaacgcaatt accgctggaa
     1081 cggcagtggg ttacggatgc acatacagac cagttcagga ttcaaatatt gcagttctac
     1141 ctgtagggta ctttgaagga tacgataggc gactttcgaa caaggctcac gttttgattt
     1201 ccgggaaaag agctcccgtg atcggcagag tgtgcatgaa catgatcatg gtagacataa
     1261 cgcacattgc agatgcaaaa gtcggcagtg tggcgactct cattggcaca gacgagaaag
     1321 aatctgtaac tgcagatgat ctggcggatt ggatgggaac tatcaactat gaagtaacca
     1381 ctaagattga accgggaatc ccacgcatcc ttgtggactg agacattttt cctgaaatcg
     1441 acttcgacgc agtctagtcc tttctgcctg gaacggatca cgaacggatc agtcaaaaat
     1501 tattgatgtt gtacattacc tgttttatgt tgttcatgca aaggggcaag atgtgaaata
     1561 cttagcaagt cttatgatcg tcctggctgc tgcgaattgc ggtggcggga aaaagaaagc
     1621 ggccatgtgg tggatggctc tgctcggcaa tcaaaccgcg cagactgcgg aaacgacatc
     1681 ctctgcaggt gattcagtca ctacaacgaa caatgaccag accggaacaa ctggattttc
     1741 atttcagaca acaaccacag ttccggtcag cgtgactgtg gccgattcga acggaccggt
     1801 gcaaggcgct gtagtgcagg tgcaggatcc ttccgcggcc aacacagtta tttttcaggc
     1861 agtcacaaat gaaaccggcc aggcgagcgg aacgttcacg gtggataaca ctgtaacaga
     1921 agttacactc gtcgtagatg caggcggcaa tgtagtcacg cagcccgtga atacaaccaa
     1981 tctcgtcacc tgcgatcgag acgtggatta tccaggggat gttaccattt catccggcgg
     2041 agaccaggac ggagacggaa ttacagatgc aaatgatgcc tatccatccg atccaactcg
     2101 cgccacgaag gtaactattc ctggaagcgg ccagtctatc attgcatacg aagacctcta
     2161 cccttcaccg ggtgatgcag atttcaacga ctacgttgta aaggtttcca atgaagaaga
     2221 cctgaatgcg gcagggaagg tggttcgcct tcgcggtagt tatacgcaca ttgctcgcgg
     2281 tgcaggttac acccatcaat tgaaactcac tctacctgca gcggcaggcg cactgaacta
     2341 tactgtaaaa cagtacagcg cagcaggtgc acttgagtcc accacaacca atcattactc
     2401 gaatggtagt gggcttgcga ttctcggaaa ttcgaacaac acaatcactc agtccaacac
     2461 ttcttcgggg cagacattca ctcctggcaa gcgtgcagac atcgaaatca cgtttgatac
     2521 tcccgtttcg caaagtactc tgggcagcgc tccgtttgat ctctacattc acgtgaacaa
     2581 cacgggcctc gacgttcatt tccctcgcat gtattttgat gcgaacggca aagatcaata
     2641 catggacaca aacggattcc cgtgggcgct ccagattcca gaagcatgga agtggcctct
     2701 tgaatcagac aacatcgaga acggttatcc aggattccga ccctggtatc tgtcttctgg
     2761 aacgacaaat aaggactggt attcgagtgc agtcagctct cacgtattca acgcgagcag
     2821 cctgtttgct tacttgaagt ctccggcaag ctggatgatt ctcggactca ttgctgccat
     2881 tgcagtgatt ggccttgctc tcgcaatgat tcagagacgg cgagcggcct gaattttgag
     2941 atgagcgcga gagcgctctt ttccagtgga atacgcagcg cgaatcatac tatccaccat
     3001 tccggttggt ttatttttcg cgctctactt ccgtaacttc gtatttcaaa gatctattct
     3061 ctggcaatcg aaagcgttct ttcttggaat gctttcgagc attgccgcca ttgttatcca
     3121 ggcccttctt cccgagaccg caagcccggc ggttcgatct ttttttcacg cagccctggt
     3181 agaagaggca atccggttca ctgttctcta tctgcgtatc cgaaagagtt cggaaggatt
     3241 tacagtcacc gaaggaatct tcgacggtat tctcgtagcc cttggatttg cgttttccga
     3301 aaatcttcac tacagcgcga ctacgagcgg ttatgtgata ttgctgcgat gcattagctc
     3361 tgttccactt catgtgtttt gcggtgggat catggcatat tttctttctt acagaagcct
     3421 ctgtcgcgaa aacaggaaag tctggcgcag actaaactgg tacgggctca gacgattcga
     3481 gctcgcagtc tttgccctcc tgattccgtt tttgctgcat ggttttttcg actacgcgtt
     3541 gtttcgcgga gatgtctgga actacatgct cgttcccatt ctggtcactg gatttatcgt
     3601 aacagaatac atggcagctc gtggcatgca gatttttgga aaaaacattc tgggggcgct
     3661 tggactggat gcagatga
//
LOCUS       DAOQJL010000400         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15814_length_3671_cov_3.905697, whole genome shotgun sequence.
ACCESSION   DAOQJL010000400 DAOQJL010000000
VERSION     DAOQJL010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15814_length_3671_cov_3.905697"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2253)
                     /locus_tag="PLG78_15005"
     CDS             complement(<1..2253)
                     /locus_tag="PLG78_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE24481.1"
                     /translation="MLVRGVILLIATATATFAQSIAIGDFESLGGKPEVELASTIRNL
                     LLSQFVAEGFRAELVSVPSSDMPVAQGADLTIVGQYIRTENGLLLYGQIFTERGMIEA
                     YSLETDLPLYRLNDQEMLESDRARIERFGKRVVARVKTNPRSILRADNIAEHFKGTRV
                     GTRPAKIAKADADSQDAFKVFREGISVASNVADRAEKQPAAVTVITRSQVKSSGARTV
                     NELLTTFVPGYFAVEDQDDVIAGFRGLAPDNNSKVLLLINGHNMNAEWFGGPPDSLLN
                     GMSLEYIERIEVIRGPGSVTLGQGALLGVINIVTRNADNAPGVGFYGAGGQNGFGSGS
                     IQAGATGQVIEDLKAFVLFQATNYSGQKLRDEGWAHDRTFEGVEGWYDARVKPGTAST
                     LEIYKDPAQSGSRLKRGNNRIALAELDYAGLAYEGIYTDQLRDYYNFYRDRDEYRNVV
                     FSNDVSYKFQFSPNVFAKARANYATDDSIYQSHGGHIMGGTREARYGGSTILRWNAAH
                     NRNRLGVGGEYRRYDMGQPDVNGNNFIVNKADTTLFDDVNRNHRYVFPSSIIVYSAFA
                     EDFFALTPSLEFFGAVRYDKHPYWGGNLSPRIGLFYDFARNLRTRFSYQEGFRGSTGV
                     SYSGGFQGDGLLRASNYDKVALSRIPTTDQFNQPTYYGNIANLEPEKIRTYELAFNYE
                     PGDRLSFDAVTFYNTIQKVIDVGVIYADPTAFKMPRIGTDVAGDWDGYFFFRNLPGVI
                     HAGGAEASGTY"
     gene            2400..3107
                     /locus_tag="PLG78_15010"
     CDS             2400..3107
                     /locus_tag="PLG78_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNE24482.1"
                     /translation="MRLIVIAVLSLLSCQKWNEMIGKHWSYEKPEEWGASCNTGRLQS
                     PVDIQKTTHANLESVRIDYSDTPLAITNNGHTIQVNAAGAGGITVGETRFNLVQYHFH
                     ARSEHAIQGATADMEVHFVHKDAAGNIAVVGVLLRKGESNPLLKAIVENAPKEKGEKT
                     VSTRGNPALLLPPSRGYYTYVGSLTTPDCSEGLKWIVMKMPVEVSEQEIALFRAMYAN
                     NARPVQALNNRIILESE"
     gene            3174..>3671
                     /locus_tag="PLG78_15015"
     CDS             3174..>3671
                     /locus_tag="PLG78_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24483.1"
                     /translation="MLWIKNHKKTTGLLVALLLLFAVFLRLSAWDDSTRPSADLSQVH
                     PESTPGTLRDVVVVGNNWDGTADVFDPTTFKKLKRFDIVPDFDERMAEVNSSRIRRLF
                     FRLIRNLIGEGHNQLVDDMFTSHDGKFLFVSRPSFADVISVEVQTGKIVWRTKVDGNR
                     ADHAAI"
BASE COUNT          944 a          992 c          891 g          844 t
ORIGIN      
        1 gtaggtcccg cttgcctccg cacccccggc atggattaca ccaggtagat ttctaaaaaa
       61 gaaataacca tcccagtctc ccgccacgtc cgtgcctatg cgaggcattt taaatgcagt
      121 cggatctgca tagataacac ctacatcaat caccttttga attgtgttgt agaacgttac
      181 cgcgtcaaag gaaaggcgat cccctggttc gtagttaaat gcaagctcgt aagtacggat
      241 cttttctggc tccagattcg caatgttgcc gtagtaagtg ggctgattga actgatccgt
      301 tgtgggaatt ctggacaggg ccaccttatc gtagttagac gcccgcagta ggccatcccc
      361 ctggaaacct cccgaatacg atacccctgt agatccccgg aacccttcct gataagaaaa
      421 acgcgtacgc agattgcggg caaaatcgta gaatagacca atcctgggag aaagatttcc
      481 accccagtat ggatgtttat cgtatctcac agcaccgaaa aactccagag aaggagtgag
      541 agcaaagaaa tcctccgcga atgcggaata cacgatgatc gaggaaggaa aaacgtatct
      601 gtgatttcta tttacatcgt cgaataacgt tgtgtcagcc ttgtttacga tgaagttatt
      661 gccgtttaca tccggctggc ccatgtcata tcgcctgtac tcccctccca cacccagacg
      721 attcctgttg tgagcggcat tccagcgaag gatcgtggaa ccaccgtatc gcgcctcacg
      781 cgtccctccc atgatgtgac cgccatggct ctggtaaata ctatcgtctg ttgcgtaatt
      841 ggctctggcc ttcgcaaata cgttaggtga aaactggaac ttgtaggaga catcgtttga
      901 aaaaacaaca ttccgatact catcacgatc gcgatagaag ttgtaatagt cacgcagttg
      961 atctgtataa attccctcat aggccagacc tgcgtagtcc aattccgcaa gcgcaatccg
     1021 attatttccg cgcttgagtc tacttccgct ttgtgcagga tccttgtata tttccagagt
     1081 acttgccgtt cccggcttca ctcgagcatc gtaccagccc tctacacctt caaaagttct
     1141 atcgtgcgcc cagccttcgt cgcggagttt ctgtcccgag tagttcgtag cctgaaagag
     1201 aacgaacgct ttgagatctt ctatcacctg gcccgtggca cctgcctgaa tcgatccaga
     1261 accaaagcca ttttgaccac cagccccata aaaaccgaca ccgggtgcat tgtccgcgtt
     1321 tcgcgtaaca atgttgatca caccaagcaa agcgccctga ccgagagtga ctgaaccggg
     1381 cccgcgaatc acttctatcc tctcgatgta ctcgagactc atgccgttga gaagagagtc
     1441 aggcggtcca ccgaaccatt ccgcattcat gttgtgacca ttgatcagca gaagcacctt
     1501 cgagttgtta tccggtgcaa ggccgcgaaa accagcaatc acatcgtcct gatcttccac
     1561 tgcaaaatag ccggggacaa acgtggtcaa caactcgttg actgtacgtg cgccgcttga
     1621 ctttacctga gatcgcgtga tgacagtcac agccgcgggc tgcttctccg ccctgtcagc
     1681 gacgttagac gccacagaga tgccttcgcg aaatactttg aaagcgtcct gcgaatccgc
     1741 atccgccttc gcaatcttcg cgggcctggt ccctacccgc gttcctttaa agtgctctgc
     1801 gatgttatct gcacgcaaaa tgcttcgggg atttgttttc acgcgcgcca ccactcgttt
     1861 cccaaagcgc tcaatcctcg ccctgtctga ttcaagcatt tcctggtcat tgaggcgata
     1921 gagtggtaaa tcagtttcga gactgtaggc ctctatcatg ccgcgttctg taaagatctg
     1981 accatacaaa agaagaccgt tttctgttct gatgtactgg cccacgatcg ttagatccgc
     2041 gccctgggcc acgggcatat ctgaggaagg aacggacaca agctctgctc gaaacccctc
     2101 ggctacaaac tggcttagta aaagattgcg aatggtgctc gccagctcca cctctggctt
     2161 ccctccgagc gattcaaaat cgccgattgc gatcgattgt gcaaaagttg cagtggcagt
     2221 cgcaatgagc agaatgactc cgcgaacgag catacccgaa ggataggagc acggacactg
     2281 agtccgggca aggtcttttt tacaattgtc atgaaatctg gcggaagaca gaaccgaacc
     2341 gtgcccagag gctcgcgaaa aggtttccag aatcgcccgg atggtttttc aatggctgga
     2401 tgagactgat tgtgatcgca gtattgtccc tattgtcatg ccagaaatgg aatgagatga
     2461 tcgggaaaca ctggtcatac gaaaagcctg aagaatgggg ggcaagctgc aatacaggca
     2521 gactgcaatc ccccgtggac atccagaaaa ccacgcacgc aaatctagaa agtgtaagga
     2581 tagactactc agatacaccg ctcgctatca cgaataacgg ccacacaatc caggtaaatg
     2641 cggccggggc cggcgggatc accgtgggag aaaccagatt caatcttgtg caataccact
     2701 ttcatgcccg cagtgaacac gccattcagg gagccaccgc agacatggaa gtgcatttcg
     2761 ttcacaaaga tgcggcgggc aacattgcgg tggtaggagt tcttctaaga aaaggagaga
     2821 gtaaccccct gctcaaagca attgtggaaa acgccccaaa ggagaagggc gaaaagacag
     2881 tatctacccg cggaaacccg gcgcttctcc tcccgccttc gcgcggttat tacacgtacg
     2941 ttggctccct gaccactccc gattgctcag agggattgaa gtggattgta atgaagatgc
     3001 ccgtagaagt atccgagcag gaaatagctc tctttcgggc catgtatgcg aataacgctc
     3061 gtccggtgca ggctctgaac aatcgcatta tactcgaaag cgaatgatgg aatcgggaaa
     3121 aatgcttttc tgaccaaagg gtgagtccag cttcaggcgg gggatcaaac accatgctct
     3181 ggatcaagaa tcacaaaaag accaccggcc ttctcgtcgc attactgcta ttattcgcag
     3241 tcttcttacg cctgtctgcc tgggacgatt ccactcgccc ttccgccgat ctaagccagg
     3301 tccatccaga gtccacaccg ggcactctca gggatgtagt agtagtcggg aacaactggg
     3361 atggcacagc agatgttttt gatccaacca cattcaagaa attgaaacga tttgacatcg
     3421 ttccggattt cgacgagcgc atggcagagg taaactccag caggatccga agattgttct
     3481 ttcgcctcat tcgcaacctc attggggaag gacacaacca gctggtggac gacatgttca
     3541 cttcacacga cggcaaattc ctcttcgttt ctcgtcccag tttcgcagac gtgatctctg
     3601 tggaagtgca gacgggcaaa attgtctgga gaacgaaagt agacggaaat cgcgcggacc
     3661 atgcagctat t
//
LOCUS       DAOQJL010000401         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15956_length_3652_cov_4.170420, whole genome shotgun sequence.
ACCESSION   DAOQJL010000401 DAOQJL010000000
VERSION     DAOQJL010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_3652_cov_4.170420"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..310
                     /locus_tag="PLG78_15020"
     CDS             62..310
                     /locus_tag="PLG78_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24484.1"
                     /translation="MKKWKGQKLYAGLSFTREGRELFNDPSRVYLKTQGDVPRLLITT
                     KDNLQRASHVSPEDVHFLLCEVVDVQGPGQGDLRRQEK"
     gene            315..1043
                     /locus_tag="PLG78_15025"
     CDS             315..1043
                     /locus_tag="PLG78_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE24485.1"
                     /translation="MIEPDKNQNAIVARELPPLAAGLVHEVRNPLAAIHLHLQLLESY
                     IGQVRDPELREKMQGKARVIKTEIMNLNQTLQDFLRMLREERVEKQIGDLNTAVREVI
                     ELLEPQARQEHIDLAFRPGELGSNTVFEPTFVKQILVNLILNSVQAFEKAKQQADKRI
                     FVETGMEGKNAFFRVRDNGPGISPEVQARIFEPFFSTKEEGSGLGLSLVQKMTGEMGG
                     DVRVSSVPDEGAEFTIVLGRTGLQ"
     gene            1065..1508
                     /locus_tag="PLG78_15030"
     CDS             1065..1508
                     /locus_tag="PLG78_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNE24486.1"
                     /translation="MTDVWQLHYRSLENMYHKNAKINEFFHPELKIARMEAEVKIPVV
                     PDFFHAGGSVHGSVYFKALDDAAFFAANSIVRDVFVLTLTFQIQLTRAVRDGIMTAKG
                     RVLQNLGNQIMAESILYDERGKETARGSGVFVKSKLPLSPEIGYV"
     gene            1508..2875
                     /locus_tag="PLG78_15035"
     CDS             1508..2875
                     /locus_tag="PLG78_15035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE24487.1"
                     /translation="MPNILIIEDDPAQRSALEEFLTDYLGKKGNVFAAEDLETARTVF
                     RDRKIQLIVTDLMLPDGTGIEFVKEIKDSKIPVLLLTGQPSIETAIEAIRAGASDYLI
                     KPVDLSHLQVKITSLLENSELKEENRILKARLQDRFSAKNIVGNAQIMKPILDRIRQV
                     APTDVTVLLEGESGTGKELFANLLHENSPRADKPFIKVNCGALTKTILESELFGAVKG
                     AYTGSDRDRAGYFESANGGTIFLDEIGEMDLESQVRLLRVIEEREVVRVGSSKPAKVD
                     VRVIAATNRSLQKEVEAGKFREDLYYRLAVIRIELPALRMRKEDIPILFNHFVTGFNE
                     KYRKSVNALSPELNHFFQQYDWPGNIRQLRNVLEGMVVLAQDNSLTLADLPDELAHPT
                     RRTADKKLMDSVLPGIAMDDYEKAIILKNLSYTGGNREKAAKLLGLSERTLYRKIKDY
                     GMEND"
     gene            2868..3629
                     /gene="recO"
                     /locus_tag="PLG78_15040"
     CDS             2868..3629
                     /gene="recO"
                     /locus_tag="PLG78_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNE24488.1"
                     /translation="MIESARGIVLRRKKDRESDAVITLLCEDGRLLRARFHGILQSKT
                     RSNLTAEPGSHVELVLQSKDASIASVKEGTLLERNDVWKSGYRPLSLLSSVLEFAEGM
                     ASACDSDDLYRLIAGGMEEARVHARLFEEPESILFFTAIRIRGLTIAGLTGDLSYCAS
                     CGERLGKQARWSVPEISFLCNKCSEETTEADARAAHTLARMAAEGFSSLLSVRQGDNG
                     YIDHLKRVEQLLAICIRHALPFPSPAADALTGGPA"
BASE COUNT         1023 a          871 c          947 g          811 t
ORIGIN      
        1 ggggttttga gcggaatgac ctggaaggac gccaatcgca gacgatggaa ctgcaaactc
       61 tttgaaaaaa tggaagggac aaaagctcta tgctggcctt tcttttacac gggaaggaag
      121 agagcttttc aatgatcctt cgcgcgttta tttgaaaact cagggagacg tacctcgatt
      181 gctcatcaca accaaagaca atctacaacg cgcatcgcat gtttcgccag aagacgtgca
      241 tttcttgttg tgcgaggtag tcgatgtgca ggggcccgga cagggagatc tccgcaggca
      301 agagaaatag ctagatgata gaaccggaca aaaaccaaaa tgcaattgta gcgcgagagc
      361 ttcctcctct ggcagccggc ctggttcatg aagttagaaa tccgctcgca gccatccacc
      421 tgcatctgca gcttctggag tcctacattg gccaggtccg tgatcctgaa ctccgcgaga
      481 aaatgcaggg gaaagcacgc gtcatcaaaa cagagatcat gaatctcaat cagactctac
      541 aggatttcct ccgcatgctg cgcgaagaga gagtggaaaa acaaatcgga gatctcaaca
      601 ctgcagtgcg cgaagtcatc gaactgctcg aaccgcaggc aaggcaggag cacattgatc
      661 ttgcttttcg tcccggcgaa ctagggtcca acaccgtatt tgaacccaca ttcgtaaaac
      721 aaatacttgt aaatctcata ctaaattcag tgcaggcttt tgaaaaagca aagcagcaag
      781 cagacaaacg cattttcgta gaaacaggca tggaaggaaa gaatgcattc tttcgcgtac
      841 gggacaatgg gcccgggatt tctccggaag tgcaggccag gatttttgaa ccgttctttt
      901 ccacaaagga agaaggaagc ggacttggac tttctctggt ccagaagatg acaggagaaa
      961 tgggcgggga cgttcgagtc agctctgttc cagatgaggg agcggaattc acgatcgtac
     1021 tgggcagaac aggattgcag taaattccga gaagaagaga tcaaatgacc gacgtctggc
     1081 agctccacta tcgcagtctc gagaatatgt accacaagaa tgcgaagatc aacgaatttt
     1141 ttcacccaga gctcaagatc gcacgcatgg aagcagaagt caaaatcccg gtggtgcctg
     1201 atttctttca cgcaggtggt tctgtgcacg gcagcgttta tttcaaggca ctcgacgatg
     1261 ctgcattctt tgccgccaat tccatagtgc gcgatgtgtt tgtactcacg ttgacttttc
     1321 agattcaatt aacaagagct gttagagatg gaatcatgac tgcgaaaggt cgcgtgcttc
     1381 agaatcttgg aaatcaaatc atggcagaat ccattctgta cgatgaacga ggaaaagaaa
     1441 cggcacgcgg cagcggcgtc tttgtcaaaa gcaaacttcc actgtcccct gaaattggtt
     1501 acgtctgatg cccaacattc tgatcataga agacgatccg gcgcagcgct cagccctgga
     1561 agaatttctc acagactacc tgggcaagaa gggaaacgta tttgcagccg aagatctcga
     1621 aacagctcgc actgtatttc gggacagaaa gattcaattg attgtcaccg acctgatgct
     1681 gcccgatgga actggaatcg aattcgtaaa ggaaatcaaa gattccaaaa tcccagtgct
     1741 tttactgaca ggacagcctt cgattgaaac agccatcgag gcgattcgcg cgggggcatc
     1801 cgactatctg atcaaacctg tggacttgag ccatcttcag gtcaagatca cttccctcct
     1861 tgaaaattca gaactgaaag aagaaaacag aattctgaaa gccagactgc aggatcgctt
     1921 cagcgcgaaa aacattgtag ggaatgctca gatcatgaag ccaattctcg acagaatccg
     1981 tcaggtggct ccgacagacg ttacagttct cctggaagga gaaagtggta caggaaaaga
     2041 actcttcgcg aacttgctgc atgagaactc ccctcgcgcg gacaagccgt tcatcaaggt
     2101 gaactgtggc gcgttgacaa agacaattct agaaagcgaa ttatttggag ctgtgaaagg
     2161 tgcttacacg ggaagcgatc gggatcgagc cggctacttt gaatccgcga atggtgggac
     2221 tatttttttg gatgaaattg gagagatgga cctggaaagc caggtgcgtc tgctgcgagt
     2281 gattgaagaa cgagaagtgg ttcgagttgg atcttcaaaa ccggcaaagg tggatgtgcg
     2341 cgtgattgcg gccaccaatc gttctttgca aaaggaagtg gaagcaggaa aattcagaga
     2401 ggacctctac tacagactcg ctgtgattcg aatcgaactt ccggcccttc gcatgcgaaa
     2461 agaagacatt cctatcctgt tcaatcattt tgtgacaggg ttcaacgaaa agtatagaaa
     2521 atcggtgaac gcactttcgc cagaactcaa tcacttcttc cagcaatatg attggcccgg
     2581 aaacatccgg caattgcgca atgttctgga gggcatggtc gtgctggctc aagacaattc
     2641 tctcacgctt gcagaccttc cagacgaact cgctcatcca actcgcagga ccgcggacaa
     2701 gaagttgatg gattctgttt tgccaggaat tgccatggac gactacgaaa aagcaatcat
     2761 cctcaaaaac ctttcttaca ccggcggaaa cagagagaaa gccgcaaagt tgctgggcct
     2821 ttccgaacgc acattgtatc gcaaaatcaa agactacggt atggagaatg attgagtcgg
     2881 ctcgcggcat cgtacttcgc agaaagaaag accgagagtc tgatgctgtc atcaccttac
     2941 tatgcgaaga cggaaggcta ctccgtgcac gctttcacgg aatcttacag tccaaaacca
     3001 gaagcaacct gacggcagag cctggatcgc atgtagaact ggttctccag tccaaagacg
     3061 catccatcgc cagtgtgaaa gaaggaactc tcctggagag aaacgacgtc tggaagagcg
     3121 gctatcgtcc gctcagcctg ctttcatcgg tgctggaatt cgcagaaggt atggcgagcg
     3181 cgtgcgattc ggatgatctc tatagactca tcgcaggcgg gatggaggaa gcgcgagtgc
     3241 acgcaagatt gttcgaagag ccagaatcga tcttattctt cacggcaatt cgaattcgcg
     3301 gcctcacgat tgcaggttta accggggatc tatcctactg cgcatcgtgc ggagaaaggc
     3361 tcggaaaaca ggcacgctgg tccgttccgg aaatttcctt tctctgcaac aagtgcagcg
     3421 aggagactac ggaagcggat gcaagggccg cacacactct ggcgcgcatg gctgcggaag
     3481 ggttttcatc tcttttgtcc gtgcgccagg gagacaatgg ctacatcgat catctgaaac
     3541 gagtggaaca gttgcttgcc atttgcattc gccatgctct tcctttcccg agccccgcag
     3601 ccgatgcttt gactggaggc cctgcatgaa gcgattcttt cagttcctta tg
//
LOCUS       DAOQJL010000402         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15967_length_3651_cov_4.312570, whole genome shotgun sequence.
ACCESSION   DAOQJL010000402 DAOQJL010000000
VERSION     DAOQJL010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_3651_cov_4.312570"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLG78_15045"
     CDS             <1..185
                     /locus_tag="PLG78_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE24489.1"
                     /translation="AAMSGLNAGIAESLVATAAGLFVAIPATISFNLFRRSADDLVTQ
                     LEIAASRLRTLLERSK"
     gene            185..586
                     /locus_tag="PLG78_15050"
     CDS             185..586
                     /locus_tag="PLG78_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNE24490.1"
                     /translation="MVHYRANKTQLFSGINIVPFTDVVLVLLIVFMISAPGLLNTAMN
                     IKLPGSTQATKRSDTKITVGLDASGKIFVNNEVVAREKVQEKVKELLKGESGEILLNA
                     DMKSHHGEVVELLDLLRQSGGNNVFIGTVRK"
     gene            593..1759
                     /locus_tag="PLG78_15055"
     CDS             593..1759
                     /locus_tag="PLG78_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24491.1"
                     /translation="MLKRSRRLPEAISILAFLLPLPLLFPLYPLSMFVLIGSLFETAL
                     SAAVSALLLREQIHPAQRKKAEFLIRAVGLPLTVVCFAGSSTLPMAGAMRLILCSALL
                     AAFSIKRAVSLVALFAVGIVLFTVARSFPEFPYFDFSHLYIAVSLSALAIIAARAGFV
                     FAARVRRSRATARAALRHARKVEERTTLFVARSVGTEDATSFLRVGSIPSRESTAALV
                     SLYMYDVVDAASSFGPDAHRSAFDAEWDGFTGRLVSLGERLRAQAQWDGDALRFRLKS
                     MSEAFGLAQELLQFVRRTSLHQQTQGRRWVTSVCVVSEGSLFQLAGAPEMPLALLRGP
                     LREDHEQFIRSLTDRDLEILTGEFLPVVPASFHPPGLERRPFGDWAIAHRPGGS"
     gene            1806..3107
                     /locus_tag="PLG78_15060"
     CDS             1806..3107
                     /locus_tag="PLG78_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNE24492.1"
                     /translation="MSMNQLETIALRAGQVPDPVTKSRAVPLYQTTSYVFDDADHAAR
                     LFGLQEFGNIYTRIMNPTTDVLEKRIAALEGGVGALATASGQSAETLAILNAAEAGDE
                     IISSSSLYGGTYNLFHYTLPKMGIQVKFVDPSKPEDFKKAITAKTRLIFAETLGNPKL
                     DVLDFEAVSKIAHAEGLPLFIDNTLASPFLLRPFDHGADIVVHSTTKFIGGHGTSIGG
                     IIVDSGKFARNQGRHKMFTEADESYHGLKFWDVFGKFEPFGGVNIAYILKARVQGLRD
                     IGAAMSPFNAWMFLQGLETLHLRMERHSQNAMKVAQFLKSHPKVSWVNYPGLPDHPSY
                     NLAKKYFYRGLFGAILGFGIKGGKEAGKKFINNLKLFSHLANVGDAKSLAIHPATTTH
                     SQLTAAEQVATGVSDDFVRLSVGIEGSEDIEADLKQALDKI"
     gene            3189..>3651
                     /locus_tag="PLG78_15065"
     CDS             3189..>3651
                     /locus_tag="PLG78_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE24493.1"
                     /translation="MPGITTQSGYTFPYIELSYLTIGKLSPAKDNAILICHALSGNAQ
                     VAGMMESGKPGWWDFHVGPGKTIDTNHFFVICSNVIGGCNGSTGPGSIDPQTGQPYGM
                     SFPPVTIRDMAAAQVKLLDHLGVDKLFAVTGGSMGGMQALVWATEYTERVRC"
BASE COUNT          832 a          898 c          984 g          937 t
ORIGIN      
        1 atgctgctat gtcgggcttg aatgcgggca ttgccgaatc actcgttgcc actgcagccg
       61 gtctctttgt tgctattccg gccacgattt ccttcaatct gtttcgccgc tctgcggatg
      121 atcttgtgac tcagcttgag attgctgcct ctcgccttcg caccttactt gagaggagta
      181 agtagtggtt cattaccgcg cgaataagac gcaacttttt tccgggatca acattgttcc
      241 attcacggat gtggttctgg ttttgctcat cgtgtttatg atttcggcgc ccgggcttct
      301 gaacactgcg atgaacatca aattgcctgg ctccactcag gccacgaaaa gatcggatac
      361 taaaatcact gttgggctgg atgcgtccgg aaagatattt gtgaacaacg aagtagtggc
      421 tcgcgagaag gttcaggaaa aagtcaagga actactcaaa ggagagtccg gagaaatctt
      481 gctcaacgct gatatgaaat cacatcatgg cgaagtagta gaacttctgg atttgctcag
      541 acagtctggt ggaaacaatg ttttcatcgg aacagtgcga aagtaggttc gcgtgctgaa
      601 gagatcgcgc cggctaccgg aagccatttc cattctagct tttctacttc cattgcctct
      661 tctgtttcca ttgtatccgc tcagtatgtt cgtattgata ggatctttgt tcgaaactgc
      721 tctttctgcg gctgtgtctg cgctgctgct cagagagcag attcatccgg cgcaaagaaa
      781 gaaagcggag tttctgattc gagcggtggg tttgcctctg acggttgtct gttttgccgg
      841 aagttcgact ttgcccatgg ccggcgccat gaggttgatt ttgtgctcgg ctcttctggc
      901 ggctttttcg atcaagcgcg cggtttctct cgtggcgtta tttgccgtgg gcattgtact
      961 ttttacggtg gccaggtctt ttcctgaatt tccctacttt gatttttctc atctgtacat
     1021 cgcagtttcc ctgtctgcgc tcgccatcat tgcagccaga gcaggattcg tattcgcagc
     1081 aagagttcgc agaagtcgcg ctacagcacg cgccgctctt cgccacgcgc gtaaagtaga
     1141 ggagagaacc actttatttg tggctcgatc cgtaggaact gaggacgcga cgagctttct
     1201 tcgagtagga agcattccat ccagggaatc cactgcggca ctcgtttccc tgtacatgta
     1261 tgacgttgta gatgcggcta gctcttttgg cccggacgcg catcgatctg cctttgatgc
     1321 agaatgggat gggtttacag gcagactggt ttctctcgga gagaggcttc gcgcgcaggc
     1381 gcagtgggat ggagatgccc tgcggtttcg tctgaagtcg atgtcggaag cctttggcct
     1441 ggcacaggag ctcttacagt ttgtgagaag gaccagtctg caccagcaga cgcaagggag
     1501 gcgctgggta acttccgtgt gtgttgtcag cgaaggatct ctctttcagc tggcgggcgc
     1561 acctgaaatg cctctcgcgc tcttgcgcgg gccattgagg gaggatcacg aacaattcat
     1621 tcgatctctt acggacaggg acctggagat actgacaggg gaattcttgc ctgtcgtacc
     1681 tgcgtccttt caccctcccg gattggagcg caggcctttc ggagactggg cgatcgcgca
     1741 tcgacccggg ggttcttgag aaagggttta cacgagtcgc gcaacaagga attgagggtt
     1801 accatatgag tatgaatcaa ctagaaacca tcgcattgcg cgcaggccag gtgcctgatc
     1861 cagtcacaaa atcaagggca gttccgctct accagaccac ttcgtatgtc ttcgacgacg
     1921 cagatcacgc ggctcgattg ttcggtctgc aggagtttgg aaacatatac actcgcatca
     1981 tgaatccaac aacagatgtg ctcgaaaagc gcatcgcggc actcgaaggt ggagtcggcg
     2041 ctcttgctac tgcatccgga cagtctgctg aaaccctggc catcttgaat gcagcggaag
     2101 caggagatga aatcatttcc agttccagtt tgtatggtgg tacatacaat ctgtttcact
     2161 atactttacc aaaaatgggc attcaggtga agtttgtaga tccgtctaaa ccggaggatt
     2221 tcaaaaaagc aatcactgcg aagacccgtt tgattttcgc ggaaacgctt ggaaatccga
     2281 aactggatgt gcttgatttt gaagctgtga gcaaaatcgc acatgcggaa ggtctgcctc
     2341 tcttcattga caatactctg gcttccccgt tccttctgcg tccgttcgat catggagcgg
     2401 acattgtagt tcattccact acaaaattca tcggtggcca cggtacttcc attggcggga
     2461 tcatcgtcga ttccggtaag ttcgcgcgga accagggtcg tcacaaaatg ttcacagagg
     2521 cggatgaatc ctatcacggt ctgaaattct gggatgtgtt tggtaagttc gaaccattcg
     2581 gcggagtcaa cattgcctac attctcaagg caagagtgca aggtttgcgc gacataggag
     2641 cggccatgtc tcctttcaat gcctggatgt tcctccaggg gctcgaaact ctgcatcttc
     2701 gtatggaaag acacagccag aatgcgatga aagtggctca attcctcaag tcacatccaa
     2761 aagtatcctg ggttaactat cctggacttc cggatcatcc ttcctacaat ctggcgaaga
     2821 agtatttcta cagaggtctc tttggagcca ttcttggatt tggaatcaag gggggaaaag
     2881 aggcaggaaa aaaattcata aataatctga agcttttctc acatctggca aacgtgggag
     2941 acgcaaagag tcttgccatt caccctgcca ccaccactca ttcgcagctg acggcggcgg
     3001 aacaggtagc cactggtgtg tctgacgatt tcgttcggtt gtcagtcgga atagaaggct
     3061 ccgaagacat agaagcggat ctcaagcagg ctctggacaa gatctaattg cagagcgaag
     3121 atcttcaaga ttccgggcgg cgtgactacc tgtcgccgct tggcagactc gacccgactg
     3181 tagttcgatt gcccgggatc acaacgcagt cgggttacac gtttccgtac atagaactgt
     3241 cctatttaac gattggtaaa ctttcgcccg cgaaagacaa cgcgatcctg atctgtcatg
     3301 cgctcagcgg gaacgcacag gtcgcaggca tgatggagtc cggtaaaccg ggctggtggg
     3361 actttcacgt tggtccgggc aagacaatcg acacaaatca tttcttcgta atctgttcga
     3421 acgtcatcgg tggctgcaac ggttccaccg gaccgggaag catagatccg caaacagggc
     3481 aaccgtacgg aatgtctttc ccacctgtga cgattcgcga catggcagca gcgcaggtga
     3541 agcttctcga tcatctggga gtggataagc tgtttgctgt tacaggcgga agcatgggtg
     3601 gcatgcaggc gctcgtctgg gctacagaat acacagagcg tgtgcgatgc t
//
LOCUS       DAOQJL010000403         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_15969_length_3651_cov_3.260289, whole genome shotgun sequence.
ACCESSION   DAOQJL010000403 DAOQJL010000000
VERSION     DAOQJL010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15969_length_3651_cov_3.260289"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLG78_15070"
     CDS             <1..588
                     /locus_tag="PLG78_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE24494.1"
                     /translation="CSNRANQGRKRHKNRGATSSIYMSEKLGGRQIGTLAQVMGTQSG
                     WCVAHSVPDQKAGTLGPLIRQWIPHNAAVFSDEMYTWLWGIYKNHRMVNHSLKSKDSR
                     YRFSRERWSRKGVCSATAEALNNSLKTAFRNYTYVRPQYSQMYLDEWCWWKNVKYFGL
                     EKVKECGESMGSIKLRVLLLQHAPPRSRLRLRVCG"
     gene            complement(518..1534)
                     /gene="fliG"
                     /locus_tag="PLG78_15075"
     CDS             complement(518..1534)
                     /gene="fliG"
                     /locus_tag="PLG78_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNE24495.1"
                     /translation="MLAGKKSSLTGRQKAAIFLVTLGPDVASDIFKHLREDEIETLTF
                     EMARLDKVQPEDKEGVLVEFNELMMAQEFITSGGIDYAREVLERALGTQKAIDIINRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQNEHPQTISLILCYLEPQKAASILGALPHQIQAD
                     VARRVATMERVSPDVLREVERVLERKLSTLASEDYTTAGGVDTVVEILNNVDRGTEKT
                     IIEALEEEDPELAEEIKKKMFVFEDIVLLDDRAIQRVLREVDNQELAKALKSVEAEVQ
                     EKIFKNMSKRAASLLREDMDFMGPVRLKDVEDCQQKIVNIIRKLEDAGEIVVARAGEE
                     ELVV"
     gene            complement(1665..3356)
                     /gene="fliF"
                     /locus_tag="PLG78_15080"
     CDS             complement(1665..3356)
                     /gene="fliF"
                     /locus_tag="PLG78_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNE24496.1"
                     /translation="MPEGLKNVLDRITQLWTGLDNTKKLAIGGVLAAVVIAFIAMAGY
                     SSKPSKELLYDNLSATDYASISKALEAMGVEYSGSGTSAIYVDGHRRQEIVTKLAQEN
                     LIPAGVEGWEIFNMSRWDETSFDKDVKLHRAIKGSLEKMLMTLEYVKSARVELAIPKR
                     NNFLTDTDPVKASVILSLKPGMESITRKQVMGVKNLIYRSIPGLTRENITITDQNGKE
                     FVEPDEIDEAQRKLDLVDRKKAFEEKERRKWYDEIKTSLNEFYQADRVSIVRVALDMN
                     WDEVTEKQHLVTPVEESPENPDTPYPDRKIMPGGTLTLSQNARKENFKGNGFTPGGPT
                     GTEQQLPPGYRDLDYQRSEYGNQDVIQNNDYNRIEREVTRQPWEERSRSIAVMVDGSW
                     VRTGYKPDGSGWERKYTAPSEEELRTLEKLLKASMLYKGSRGDQIVVAHLQKDRTAEF
                     AEEDNEIRQKLLMQRLLIVSVIAVVALFLIYMLYRAIRREISRRRRMREEELAAQQQL
                     MREAALRVADEGAAEVELSVDERARREMLENAMNLAREKPDQVAKLLRTWLSQDE"
     gene            complement(3515..>3651)
                     /locus_tag="PLG78_15085"
     CDS             complement(3515..>3651)
                     /locus_tag="PLG78_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNE24497.1"
                     /translation="IYDPDSVEVHDVMIAAEKSRFALTLTKTVADGLVRTFRELTQPR
                     "
BASE COUNT          790 a          918 c          926 g         1017 t
ORIGIN      
        1 tgctcaaaca gggcaaatca gggacgcaag cggcataaga atcgtggcgc cacgagttca
       61 atctatatgt cagagaaact cggcggacgt cagatcggaa ctctcgccca ggttatgggc
      121 acacagtccg gctggtgcgt tgctcattca gtgccggatc agaaagccgg aacgctcggg
      181 cctctcattc gtcagtggat tccgcataac gccgctgtgt tcagcgatga aatgtataca
      241 tggctctggg gcatctacaa aaatcatcgc atggtgaatc attcgttgaa gtcgaaagat
      301 tcacgttatc gattttctcg tgagagatgg agccggaagg gagtgtgctc ggcgactgcc
      361 gaggcgctga acaattctct gaagactgcc tttcggaatt acacgtatgt gcggccgcaa
      421 tattcgcaga tgtatctgga tgagtggtgc tggtggaaga atgtgaagta cttcgggttg
      481 gagaaggtga aggagtgtgg ggagagtatg gggagtatta aactacgagt tcttcttctc
      541 cagcacgcgc caccacgatc tcgcctgcgt cttcgagttt gcggatgatg ttcacaattt
      601 tctgctggca gtcttccacg tctttcaagc gcacaggacc catgaagtcc atatcttcgc
      661 gcaaaagaga tgcagcgcgc ttggacatgt tcttgaagat tttttcctgc acttccgctt
      721 ccacggactt cagtgctttt gcaagttcct ggttgtccac ttcgcgcaga actcgctgga
      781 tcgcacggtc atcgagtaga actatgtctt cgaatacgaa catcttcttc ttgatttctt
      841 cggcgagttc cggatcttcc tcttcgagag cttcgatgat cgtcttttca gttccgcggt
      901 ctacgttgtt tagaatttca accacagtgt ccacaccacc cgcagtggtg tagtcttccg
      961 aagcaagagt agaaagctta cgctcaagaa ctcgctccac ttcgcgaaga acgtcaggcg
     1021 acactcgttc catggtcgca acacgccgag ccacatccgc ctgaatctgg tgaggcagag
     1081 ctcccagaat ggacgctgct ttctgcggtt caagataaca gagaatgagt gaaatcgtct
     1141 ggggatgttc attctggata aagttcagta gatgagacgg gtcagtgcga cgaatgaagt
     1201 caaacggtct tacctgaagg ctcgacgtca ggcggttgat gatgtcgatt gccttctgcg
     1261 ttcccagcgc tctctcgagt acttcgcgag cgtagtcaat gccgcccgaa gtgatgaact
     1321 cctgggccat catgagttca ttgaactcta caagcacgcc ctctttgtct tctggctgga
     1381 ctttgtccag acgagccatt tcaaaggtga gtgtttctat ttcgtcttcg cgcaaatgct
     1441 tgaagatgtc tgaggccacg tcagggccca gagtgaccag gaagatggcg gctttttgcc
     1501 tgcctgtaag tgatgatttc tttcccgcga gcattggatg gcgccgatat cattgtcggt
     1561 ccatttccag gtccaggcaa gtcaaaaaaa aaggcacggt ctcccgtgcc tcaggttatc
     1621 tggtgtatcc ggccggtgcc ctctggcagg agggcttcct aatattactc gtcctgtgac
     1681 agccaggtgc gaagaagctt ggccacttga tctggctttt ctcgcgccag gttcattgcg
     1741 ttctccagca tctctcgcct tgccctttca tccacagaca gttccacttc tgcggctcct
     1801 tcatccgcta cacggagtgc cgcttcgcgc atcagttgct gctgtgctgc aagttcttct
     1861 tctctcattc tgcgtctgcg agagatctcg cgacgaatcg ctctgtagag catgtagatc
     1921 aggaagagag caacgactgc aatgacagaa acaatgagca gtctctgcat caggagcttt
     1981 tgacgaattt cattgtcctc ttctgcaaat tcagcggtgc gatctttctg aagatgagcc
     2041 accacaatct ggtctccgcg tgagcctttg tagagcatgg acgctttgag aagcttttcc
     2101 agtgtgcgaa gttcttcctc tgatggggcg gtgtattttc tttcccagcc tgaaccatct
     2161 ggtttgtatc cggtcctgac ccagcttccg tctaccatca cagcgatgga tctagaacgc
     2221 tcttcccatg gctgtcttgt gacttcgcgt tcgatgcgat tgtagtcatt gttctggatt
     2281 acatcttgat tgccatactc ggaacgctgg tagtccagat cgcgataccc aggagggagc
     2341 tgctgctctg ttccagtcgg cccacctgga gtaaatccat tgcctttgaa attttctttg
     2401 cgggcattct gagaaagagt cagagttccg cctggcatga tcttacgatc tggataaggt
     2461 gtatccgggt tttctggtga ctcttccact ggagtcacca ggtgctgctt ttctgttact
     2521 tcatcccagt tcatatcgag agcaacgcga acgatgctca cgcgatctgc ttgataaaat
     2581 tcattgagag acgttttgat ttcgtcgtac catttacggc gctctttttc ctcgaacgct
     2641 ttcttgcgat ccactagatc gagtttgcgc tgcgcttcat cgatttcgtc tggttctacg
     2701 aattcctttc cgttttgatc tgtgatcgtg atgttttcac gagtcagacc cggaatagaa
     2761 cgatagatca agttcttcac gcccatgacc tgtttgcgcg tgatgctttc catgccaggc
     2821 ttcagagaga gaatcacgga agctttgact ggatccgtat ccgtaaggaa gttgttgcgc
     2881 tttggaatcg ccagttctac gcgagcgctc ttcacatact caagagtcat gagcattttc
     2941 tcgagagaac ctttgatcgc gcgatggagt ttgacatctt tgtcaaagct cgtttcatcc
     3001 cagcgagaca tgttgaaaat ctcccaacct tctactccgg ctggaatcaa gttctcctga
     3061 gcgagtttgg tgacgatttc ctgacgacga tgtccatcga cgtagatggc agaagtgccg
     3121 cttccgctgt attctactcc cattgcttcg agtgcctttg aaatacttgc gtagtcagtg
     3181 gcactcagat tgtcatagag cagttctttg gacggtttgg acgaatatcc tgccattgcg
     3241 atgaatgcaa tgacgacggc tgccagcacg cccccaattg caagcttctt ggtattgtcc
     3301 agcccggtcc agagctgagt gattcggtcg agaacgtttt ttaaaccctc cggcatggta
     3361 caccttcctg tatttgttgc ttccaagaga cataacgtgc cagaaagtac aagaaaaatt
     3421 ttatagaaac cagggaattt ttataaattt tctgtacgta cggcgaagac gccgcgccac
     3481 cctttcgggc gcgcggattc tctttaaggt tggattatct tggctgtgtg agttcgcgga
     3541 atgtgcggac caggccatct gcgactgtct ttgtaagagt cagtgcaaat ctgctctttt
     3601 ctgcggcgat catcacatca tgaacttcca cagaatctgg atcgtagatt g
//
LOCUS       DAOQJL010000404         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16018_length_3644_cov_4.464753, whole genome shotgun sequence.
ACCESSION   DAOQJL010000404 DAOQJL010000000
VERSION     DAOQJL010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16018_length_3644_cov_4.464753"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLG78_15090"
     CDS             complement(<1..215)
                     /locus_tag="PLG78_15090"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNE24498.1"
                     /translation="MKRLTYISNFSQPLARNEIERIGEVSVRNNARDGLTGALFCFHD
                     VFYQVIEGEEQAIERCYSRILKDSRHK"
     gene            318..1112
                     /locus_tag="PLG78_15095"
     CDS             318..1112
                     /locus_tag="PLG78_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNE24499.1"
                     /translation="MDYLIKDLSKMTGIASHAIRKWQERYNILRPFRTPNGYWHYSND
                     DYFVLKSIQMRLDQSQKLKKIMALGREALLKDKVTTFSEEQLTFIKLLQAHDYDSVEK
                     IFDKQLKGSFSAWVHHIIGPAVILVGKAWEENLLSVPEEHAFSHWLHGYIFAKIEKFP
                     PHAKPGWLVTTFPGDPHDLSSLLHYAVLRSRGISAWYTGNLPENDLLREVESGKYIGI
                     SISVVLEQPEKKVKALESAILARNPKIKIRFGGFGMSMRKKNKKAR"
     gene            complement(1120..2778)
                     /locus_tag="PLG78_15100"
     CDS             complement(1120..2778)
                     /locus_tag="PLG78_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE24500.1"
                     /translation="MHFPWTKHYDAHVPPVLEYPDSTIGELFDESVRKHGSRIAVQFM
                     GKKISYDSMEKDAASLAVHLSSKGVRPGDRVFLLLPNTPHFVAAYYAVMKIGAVVVPA
                     SPLDTAPEIQYKIRNSGARVAVFLDLLFESLEPSLPLLEAAVGCDLSDYLPFPKNYLF
                     PIKKRRLGRKMPPYNEKVIRFSEILKVKETFPCKHSSPDDLAVIIYTGGTTGISKGVM
                     LSHRALAVNMTQARTWGQVTGDDINLCALPFFHGFGMSIGLNTTFVAGGRMLLLPKWD
                     AGQAIRHFEKDGVTLFAAVPTMYNSILNHPDFSRMKNARLRGCYVGAAPVPESLKEEF
                     FRRTGGILIEGYGLTEAVTAKSANPYIGKKKEKSIGIPWPDTEFRIVDEITGEASAPG
                     VEGEIILRSPDLMMGYWQNPEATDEVLRDGWLFTGDIGRMDEDGYFYIVDRKKDLIIS
                     GGFNVYPSEIEEVLYSHPSVLEACVIGVRDTHKGEIPRAYVVLRQGEREDANALMEFL
                     GVRLIHYKVPRSFIFKDALPKSPIGKILRKELRAADPDVGSSRG"
     gene            complement(2794..>3644)
                     /locus_tag="PLG78_15105"
     CDS             complement(2794..>3644)
                     /locus_tag="PLG78_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE24501.1"
                     /translation="DLVILAGVPVDFRLNYGSQIRSSAFHIGVNRSSQDLKKNKRPSL
                     ALKADVCDFLISLSSVYSSGAGRLDAWISQLRARDLKREEEILSLSAKKAKNVNALKL
                     LFQLKNKLPDNAILVADGGDFVATAAYILEPPAPLSWLDPGVFGTLGVGAGFALGASV
                     VCPDREVWIIYGDGSAGYSIMEYDTFVRHRFPVIGLIGNDACWTQIYRDQKEILKDDI
                     ACMLLHTDYHKMAEATGATGFLIARESSVASGLTAARKAAQKKSPVLINAIIDISDFR
                     KGSISM"
BASE COUNT          974 a          949 c          863 g          858 t
ORIGIN      
        1 tctttgtgcc tggagtcttt cagaatcctc gaataacagc gctcaatggc ctgctcttcg
       61 ccttctatga cctgatagaa gacatcatgg aaacagaata gggctcctgt aaggccgtcg
      121 cgcgcattgt tcctgacaga cacctcgccg attcgttcaa tttcatttcg cgcgaggggt
      181 tgcgaaaagt tgctgatgta cgtgagccgc ttcatgtcca taaggaggca gaaggatcat
      241 caatcgccat ccattttttg tccaatgatt ctaaaaacat tagacaaaga aacgcagcaa
      301 ctgtatactg aagctcaatg gactatctga tcaaagatct gagtaaaatg acaggaatcg
      361 cttcgcacgc cattcgtaag tggcaggaaa gatacaacat tctacgaccg tttcgcacac
      421 cgaatggtta ctggcattat tcgaatgatg attactttgt actcaaaagc atccagatgc
      481 gactcgatca atcgcagaag ctcaaaaaga tcatggctct gggacgcgaa gccctgctga
      541 aggacaaagt cacaaccttt tcagaggaac agttaacatt cataaagctt ttgcaggccc
      601 atgattacga cagtgtagaa aagattttcg acaagcaact caaggggtcc ttctcggcat
      661 gggtgcacca cattatcgga ccggcagtaa ttctcgtagg caaggcatgg gaagaaaatc
      721 tactcagtgt cccggaggag catgcatttt cacactggct tcacggatac atattcgcga
      781 agattgaaaa gtttcctcct cacgcgaaac cgggatggct tgtaaccaca tttcctggag
      841 atccgcacga tctgagtagc ctcctgcatt acgcagtttt acgcagtcgg ggaatctctg
      901 cctggtatac cggcaactta cctgagaatg atctccttcg cgaagtagaa tcaggaaagt
      961 acatagggat ttcgatatca gtagtcctgg agcagccgga aaaaaaagtc aaagcactcg
     1021 aaagcgccat cctcgcacga aacccgaaga tcaagattcg ctttggtgga ttcgggatga
     1081 gcatgagaaa gaagaacaaa aaggcacgtt aacttaccat catcctctac tcgatccaac
     1141 gtccggatca gcggcacgga gttcttttct caagattttc ccaatgggac tttttgggag
     1201 tgcgtctttg aaaataaaag aacgcggcac tttgtagtga atcaaacgca ctccaaggaa
     1261 ttccatcagc gcattggcat cttcgcgctc cccctgacga agcacaacat acgcgcgggg
     1321 aatttcgccc ttgtgagtat ctcgcactcc gatcacgcac gcttccagca cggatggatg
     1381 actgtacaga acctcttcaa tctccgatgg atatacgtta aaaccaccac tgatgattag
     1441 atccttctta cgatctacaa tgtagaagta tccgtcctca tccattcttc cgatatcacc
     1501 tgtgaacaac cacccgtcgc gtaacacttc gtccgttgcc tctggattct gccagtagcc
     1561 catcattaaa tctggactgc gcagaatgat ttcaccctcc actcccggcg cggatgcttc
     1621 cccggtgatc tcgtccacaa ttctgaactc agtatccggc cacggaatcc caatcgactt
     1681 ttccttcttc tttccaatgt acggattcgc ggattttgca gtcacagctt ccgttaggcc
     1741 gtagccttcg atcaagattc ctcctgtgcg acggaagaat tcctctttga gcgattcggg
     1801 cacgggagcg gcccccacat agcatccgcg caatcgagca ttcttcatgc gggaaaaatc
     1861 cggatgattc aggatgctgt tgtacatcgt tggaacagcg gcgaataacg tgacgccatc
     1921 cttttcaaaa tgacggatcg cctggcccgc atcccatttg gggagtagca gcattcgacc
     1981 gcccgcaaca aacgtagtgt tgagcccgat gctcatgccg aatccgtgaa aaaaaggaag
     2041 agcacacaga ttgatgtcat cccctgtcac ctgaccccag gtacgcgcct gggtcatatt
     2101 gactgcgagg gctctgtgcg aaagcatcac acccttggaa attccagtgg ttccgcccgt
     2161 gtagatgatg actgccaggt catccggact ggaatgtttg caaggaaagg tttccttgac
     2221 cttcaagatc tcgctaaacc gaatcacctt ctcattgtaa ggcggcattt ttctcccgag
     2281 tctgcgcttc ttgatcggga acaaatagtt tttcggaaac ggcaagtaat cagacagatc
     2341 gcatccgacc gcagcttcga gcagtggcaa agaaggttcg agcgattcga acaaaagatc
     2401 caggaacacc gcaacgcgtg cgcctgaatt gcgaatcttg tactgaattt caggagctgt
     2461 gtcgagcgga cttgcgggca caacaacggc gccaatcttc atcactgcat agtaggccgc
     2521 cacgaaatga ggagtattcg ggagaagcaa gaaaacgcga tcgcctggcc gcacgccttt
     2581 gctagaaaga tgaacggcca gagatgcggc atccttttcc atcgagtcgt aagagatctt
     2641 cttgcccatg aattgaaccg cgattctgct tccatgtttg cgaacggatt catcgaaaag
     2701 ttccccaatg gtagaatccg gatattccag aaccggagga acatgcgcgt catagtgttt
     2761 tgtccagggg aaatgcatgg gaatcctcca taattacata gaaatcgaac ccttgcgaaa
     2821 gtctgaaata tcaatgattg cgttgatgag aaccggactt ttcttttgcg cggctttgcg
     2881 agcagcagtg agcccagaag caacagaaga ttcgcgcgca atcagaaatc ctgtcgcacc
     2941 cgtggcctcc gccattttgt gatagtccgt atgcagaagc atgcatgcga tatcgtcctt
     3001 caagatttcc ttctggtctc tatagatctg agtccagcag gcatcgttgc caatgagccc
     3061 aatcaccggg aagcgatgac gcacgaaagt atcgtattcc atgatgctgt agccggcgga
     3121 tccatctcca tagatgatcc agacttcgcg atccggacaa accacacttg cgccgagcgc
     3181 aaaccctgcg cctacgccca gagttccaaa aacgccggga tcgagccagg agagtggagc
     3241 cggtggttcg agaatgtagg cggcggtggc cacaaaatct ccaccatcag cgacaagaat
     3301 cgcattatcc ggcagtttgt tttttagttg aaagagtagc ttgagcgcat tcacgttctt
     3361 cgctttcttc gcggacagag aaagaatctc ttcctctctt ttcagatctc tcgcgcgtag
     3421 ctgtgagatc catgcatcta acctgccggc tccggacgaa taaacagaag agagcgaaat
     3481 caaaaaatcg cacacgtccg ccttgagcgc cagggaaggc cgtttgtttt tcttaagatc
     3541 ctgggaactc cgattcaccc caatatgaaa ggcggaagat cgaatctgag atccatagtt
     3601 caatctgaag tcaacaggca cacctgcgag aatgacaaga tcac
//
LOCUS       DAOQJL010000405         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16040_length_3640_cov_4.394421, whole genome shotgun sequence.
ACCESSION   DAOQJL010000405 DAOQJL010000000
VERSION     DAOQJL010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16040_length_3640_cov_4.394421"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLG78_15110"
     CDS             <1..250
                     /locus_tag="PLG78_15110"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNE24502.1"
                     /translation="IESMMGDGKKHLLPEELVKREQQRVSIATARAIRAGEIISSEMI
                     SFRGPGTGFATYEINNVVGKKAVVDISEDVLIEKAMLS"
     gene            254..1513
                     /locus_tag="PLG78_15115"
     CDS             254..1513
                     /locus_tag="PLG78_15115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE24503.1"
                     /translation="MPRVSIIIRAKNEEYWLPRLLPQLKRQSYQDFEVILVDNQSTDA
                     TCAIFRKEYPSGRILEIGDFKPGRAINAGIEAASGELVVIISAHCVPAEESWLASFVS
                     AMDDTTIGAAYGRQLPMHTSHPLDKRDLLNTFGIEFRVQKKDTFFHNANSILRRSVWE
                     RFPFDEETPHIEDRLWAAQLIENQIWIAYTPGAVVYHHHGINHHANIKRAEAISQILG
                     SGRLGYSMELPEFMRAENSHTLYCFLGHDAGTHTVLLSLLGQMKDAPPGRFIVHSAQA
                     ELLRDLPVGPDVLPYAADDRERTFVEILAELIEDEAARNFFPDAVVYINLRSPGLKWE
                     LLREAIHCYYGDMYESVFWANREYSNIWQKDNGVYAQLRADYTRREKKEPVYIARYGL
                     GTVTAPALIRSGALIGNKVAILPLEDN"
     gene            1565..2284
                     /locus_tag="PLG78_15120"
     CDS             1565..2284
                     /locus_tag="PLG78_15120"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNE24504.1"
                     /translation="MTTKPRILGITLARGGSKSVPHKNIRPILGVPLIAYTIRAALAS
                     QYLTRYIISTDDPEIRDTAIRYGADAPFLRPAEFSTDTASSVSALQHAVSWVEKEENS
                     KYDYIIEMMCTNPLKTTGDIDSCIQKLVETGADSVIAVHKLDDHHPIRIKKIVDDLIV
                     DFCLPEVPEMRRQDLKPDAYIRSGSIYALSRKHLMEENRRFGSKHSRPYILPPERAIN
                     VDTEIDFLIAEKILEKAASGT"
     gene            2289..3254
                     /locus_tag="PLG78_15125"
     CDS             2289..3254
                     /locus_tag="PLG78_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE24505.1"
                     /translation="MPRILVITPVLHIAGVPEVLESAGEVTYLDDPEPSEVIGQISSY
                     DAIFTNPNKSRVYIGEEILSAGNLKAICTASTGTNHIDKKAAAARNITVLSLTEQREV
                     INRISSTAELAFALTMAQLRKVLWSHAGALEGEWDYTHYIGRQLNSLTVGVIGYGRLG
                     SLYAGYCRAFGAQVLVYDPYKKVDRADISQLATIEELFERSDVISIHVHVSPETTNMI
                     HAASFARMKKDVLLINTARGEIINESDLVGFLKNNPEARAGVDVLADEIKNRLNSPLL
                     DYAKSGRQVVITQHIGGMTREAQEIAYGHAARMLHSFLRGSANPV"
     gene            3265..>3640
                     /locus_tag="PLG78_15130"
     CDS             3265..>3640
                     /locus_tag="PLG78_15130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE24506.1"
                     /translation="MKYISEIDSIRAFACLMVMWDHLYSRFFLRIGDRELFTGGFIGV
                     DVFFVLSGFLISSLLFQEKERSGRIRYGKFYVRRALRLLPPIFTVVPLVLLPLIYYFD
                     GAAVTILNYFYLVTYTTVLPKFL"
BASE COUNT          879 a          889 c          929 g          943 t
ORIGIN      
        1 gattgagtcc atgatgggtg acgggaagaa acatctactt cctgaagagc ttgtgaagag
       61 agagcagcaa cgcgtctcga ttgcaactgc gcgcgcgatt cgcgcaggag aaatcatttc
      121 atccgagatg atttcctttc gggggccagg cacaggtttc gcgacgtatg agataaacaa
      181 tgtagtcggg aaaaaagccg tcgtcgatat ttccgaagac gttctcattg aaaaggcaat
      241 gctaagctaa cctatgcctc gtgtttccat tatcattcga gcaaagaacg aagagtactg
      301 gctgccgcgt cttcttccgc agttgaagcg tcagtcgtac caggatttcg aggtcattct
      361 ggtggataac cagagcacag acgcaacgtg cgccatcttt cgcaaggaat acccatcggg
      421 acgaatcctc gaaattggag actttaagcc gggcagagcc atcaatgcag gcatagaagc
      481 tgcgagcggg gaactcgtgg tcattatttc tgctcactgc gtgcctgcgg aggagagctg
      541 gcttgccagt tttgtttctg cgatggacga tacaaccatt ggcgcagcat acggccggca
      601 gcttcccatg cacacatcgc atcctctcga caaaagggac ttgctcaaca cattcggtat
      661 cgagtttcga gtacagaaga aggacacctt ttttcacaac gccaacagca tattgcgtcg
      721 ctcggtgtgg gagagatttc cctttgatga agagacgcct cacatcgaag atcgcctctg
      781 ggccgctcag ttgattgaga atcagatatg gatcgcgtac acaccgggcg ctgtagtgta
      841 tcatcaccac ggcatcaatc atcatgctaa cataaaacga gcagaagcaa tttcgcagat
      901 tcttgggagt ggtcgccttg gttattcgat ggagcttcca gaattcatgc gtgctgagaa
      961 ttcacatacg ctttattgtt ttctgggcca tgacgcaggt actcataccg ttcttctgtc
     1021 tttgctgggc cagatgaagg acgctccgcc aggcagattc attgttcatt ctgcacaggc
     1081 cgagcttctg cgggatcttc ccgtagggcc cgacgttctt ccctacgcgg cggacgatcg
     1141 tgaaaggaca ttcgttgaaa ttctggcgga gttgatcgag gatgaagcag ctcgcaattt
     1201 ctttccggac gcggtggtct atatcaatct gcgctctccc gggttgaagt gggagttgct
     1261 gcgcgaagct atacattgct actacggtga catgtatgaa tccgttttct gggcgaacag
     1321 agagtatagc aatatctggc agaaagacaa cggggtttac gcgcaacttc gtgccgatta
     1381 tacgcgtcgc gagaagaagg aacccgttta cattgcgcgg tatggccttg gaactgtgac
     1441 cgctcctgct ctcattcgat ccggcgcatt aattggaaac aaagtcgcga tccttcctct
     1501 cgaagacaat tgattcgcct ttgtgccaca gcctgacgtt cagctaaact atagagaaaa
     1561 tcgtatgact accaaaccta gaattcttgg catcactctg gcgcgcggag gctcgaagag
     1621 cgttccgcac aagaacataa ggcccatcct tggagttccg ctcatcgcgt acacgattcg
     1681 tgctgcgctc gcttcacaat acctgactcg ttatatcatt tccacagacg atccggagat
     1741 tcgcgacacg gcgattcgct acggtgccga cgcacctttt cttcgtcccg cggagttctc
     1801 caccgacaca gcatcgtctg tttcggcgtt gcagcatgct gtcagctggg ttgagaaaga
     1861 ggagaatagt aagtacgact atattattga aatgatgtgt acgaatccgt tgaagacaac
     1921 tggcgacata gattcttgca ttcaaaaact cgtcgagact ggcgcggact cggtcattgc
     1981 cgtgcacaaa cttgacgatc atcatccgat ccgtatcaag aagattgtgg atgacctcat
     2041 cgtcgatttt tgtctgccag aggttccgga gatgcggcga caggatttga agccggatgc
     2101 atacattaga agtggctcca tttatgcgtt gagtcgcaaa cacttaatgg aggagaatcg
     2161 tcgctttgga tcaaagcata gcagacctta cattcttcct cctgaaagag cgattaacgt
     2221 ggacacagaa attgatttct tgatcgcaga aaagatactc gaaaaagccg cgtccggaac
     2281 ctgacgatat gccaagaatt ctcgttatca cccctgtgct tcacatcgcc ggtgtacctg
     2341 aggttctcga atctgcggga gaagtcacgt acctggacga tcccgagcct tcagaagtaa
     2401 tcggacaaat ctcttcttat gatgcgattt ttacgaaccc caacaagagt cgcgtctata
     2461 tcggcgagga gatcctgtct gcaggaaatc tgaaggccat ttgcaccgct tccaccggca
     2521 cgaatcacat tgataagaaa gccgccgcag ctcgaaacat cactgtgctc tctctcacag
     2581 agcaacgaga ggttatcaat aggatttctt ctacggcaga gctcgcattc gcgctgacca
     2641 tggcgcaact tcgcaaagtg ctatggagtc atgcgggtgc tcttgaggga gaatgggatt
     2701 acactcacta cattggacgc cagttgaact cactgacggt tggtgtcatt ggctatggtc
     2761 ggctgggatc tctatacgct ggctactgcc gcgcttttgg tgcgcaagtt cttgtttacg
     2821 atccatacaa gaaggttgat agggccgata tttcccagct tgcaacgatc gaagaattgt
     2881 tcgagagaag tgacgtgatt tcgattcatg tgcacgtttc cccggagacc acgaacatga
     2941 ttcatgcggc gagttttgct cgcatgaaaa aggatgttct gctcatcaat accgcgcgtg
     3001 gtgagatcat caacgagtct gatctggttg ggtttttgaa gaacaatcct gaggccaggg
     3061 caggagtgga tgttctggcg gatgaaatca agaatcgtct gaatagtcct ttattggatt
     3121 acgcaaagtc cggaaggcaa gtcgtgatta cacaacacat cggcggaatg acgcgcgaag
     3181 ctcaggagat cgcatacggg catgctgctc gtatgctgca ttctttcctt cgcggatctg
     3241 ccaatcctgt ctgattctcc ttttatgaaa tacatttccg aaatcgatag catccgggct
     3301 ttcgcctgtc tgatggtgat gtgggatcac ctctattcca ggttcttctt gcgcattggc
     3361 gatcgagagc tgttcacggg tggtttcatc ggtgtagatg ttttctttgt actgtctggc
     3421 tttctcattt cgagtttgtt gtttcaggaa aaggaaagaa gcggtcgcat tcgttacgga
     3481 aagttttatg tacgcagagc tcttcgcctc ctgcctccga tctttaccgt cgttccactc
     3541 gttctattgc cattgattta ctacttcgac ggcgcggccg taaccatatt gaactatttt
     3601 taccttgtga cgtacaccac agtccttcca aaatttcttg
//
LOCUS       DAOQJL010000406         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16104_length_3630_cov_4.607273, whole genome shotgun sequence.
ACCESSION   DAOQJL010000406 DAOQJL010000000
VERSION     DAOQJL010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16104_length_3630_cov_4.607273"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLG78_15135"
     CDS             <1..473
                     /locus_tag="PLG78_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24507.1"
                     /translation="GGLWHGAHYPYIIWGFFHGLMLVLERFLGLGLDKVLPTKIQLPR
                     ALDIVRTGLQVVFVFILFCIGAVFFNSPTVTSAFTMLGRAFTWVPGSPLQATETLSYF
                     VIAGFLLNGIQLIEKWPSLKLSHAATIAGVYGFIVMFLLAKYAPGGRDFLYFQF"
     gene            482..1615
                     /locus_tag="PLG78_15140"
     CDS             482..1615
                     /locus_tag="PLG78_15140"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HNE24508.1"
                     /translation="MRQFKILLVPLALFAVVFALDKVFLLPIVRENFVSWKKIEPPFY
                     EARRDLYDLYKRSYQADKARGKKIGAIFGTSRSAEFNTDGIDQILHDSRTYNFGAPFA
                     CPSFFYYWYSQMKADGILPDYVILETDPLLFNERAIDYSLSYSYDSSFVIKHLELGRA
                     RGGDPWSARPGFSFDEGETFFLKRLFALYRYPPDLGVYKDNNQDLPLPLPDGTIRIWK
                     GKEFRAELLKWMGSINELKLGGIPNMLSIQLPPEKLKGDAANMAAIHLTGYSPSRTQI
                     AFYRLLTKSLAEKRIQTVVYWPVVSDAFQEKIDDARLPDFVQRARVEQEIKKVDPAGI
                     IQLIYPAELKCRTFVDSVHLSGSCFPELEKILIEKGLPAQKRK"
     gene            complement(1600..2985)
                     /locus_tag="PLG78_15145"
     CDS             complement(1600..2985)
                     /locus_tag="PLG78_15145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE24509.1"
                     /translation="MLFNSFDFLVFFFVFYAVYWQLQGEWRRRWLILSSMAFYAWWDF
                     LYLFHFTAVVLISYYCYLRVREKHSKSWFYVGLALNIANLVFFKYVATTLQWLGGVTG
                     LAAFSHAGEALHFGFPLAISFYTFQLIAILVDAWRGDIDEEIGFQNFLQYSFFFPHLI
                     AGPILRHRDYFPQIGTERFDRTQSTAGIYLILFGLLKKVVIADGISSIIDPVYSNPAK
                     FTTVGAVAALFGFALQIYADFSGYTDIARGLAKMLGYEIPENFRAPYFSYTFADLWRK
                     WHITLSMWLRDYIFFPLGGSRTSPLRSHLNVLITMILAGLWHGHSYMFFFWGVYIGAL
                     MTIERLLGIGKREFSFPAKAALWVVMFTLWSSSALFFRGHDFTSAMAMIHTLSGDTAA
                     GAVMPPNWTRFPGLVALSILVHVVQYQRERLAAPLLKYGRYLIPVIAVALFYMLVRLD
                     RSTVQFIYFRF"
     gene            complement(2988..>3630)
                     /locus_tag="PLG78_15150"
     CDS             complement(2988..>3630)
                     /locus_tag="PLG78_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE24510.1"
                     /translation="VIQRMMVRKCCDAGKPVIVATHLLESMIQNPMPTRAEATDVANV
                     VFEQADALMLSGETAVGKYPVKCIEVLDQIARRLEKEPGIGFALARPAGSFREQLARS
                     ACKLADSLKSPAIVVMTRRGILAEQVASFRPSTSIIYAFTNMSSSRRKLWLARGVVPF
                     LMDFSNDPEKNIRAAFEKLRARNRVLTGDEIVVVSDVAAGSERFTSIQIRRLD"
BASE COUNT          993 a          938 c          859 g          840 t
ORIGIN      
        1 tgggcggact gtggcacggc gctcactatc cttatattat ctgggggttt tttcacggcc
       61 tgatgctggt actcgaacgt ttcctgggac tgggtctgga caaagtcctg cccacaaaga
      121 tccaactacc tcgagcactc gatatcgttc gcaccggtct ccaggtcgtc tttgttttca
      181 tcctgttctg cattggagct gtgtttttca actcccctac tgtaacgagc gcattcacga
      241 tgctcggccg agcattcacg tgggttccgg gaagtcccct tcaggcaaca gaaactcttt
      301 cctattttgt catcgccggc ttcctgctta acggcatcca gctgattgag aagtggcctt
      361 ccctcaaact cagtcatgcg gcaaccatcg caggcgtcta cggattcatc gtgatgtttc
      421 tgcttgcgaa gtatgctcca ggcggtcgcg acttcctcta ctttcaattc taggaaacaa
      481 tatgcggcaa ttcaaaatcc ttctggtccc tctggctctg ttcgctgtag tatttgccct
      541 ggacaaagtt tttctccttc cgattgtacg cgaaaacttt gtatcgtgga aaaaaatcga
      601 gcctccattt tatgaagcaa gaagagatct ttacgatctt tacaaaagat cctaccaggc
      661 agacaaagca cgcggcaaaa agatcggggc aattttcgga acatcgcgtt ctgctgaatt
      721 caacacggat gggattgatc aaatcctgca tgattcgcgt acctataact tcggagctcc
      781 attcgcctgt ccgtcgttct tctactactg gtattctcaa atgaaagcag atgggatttt
      841 gccagactat gtgattcttg aaacagatcc tctgctcttc aacgaaagag ctattgacta
      901 ctctctgtcc tattcatacg attcgtcttt cgtcatcaaa catctcgaac tgggccgtgc
      961 acgcggtgga gatccatggt ctgcaaggcc aggattctcc tttgatgagg gagaaacgtt
     1021 tttcttaaag agactcttcg cgctctacag atacccgccg gacctgggag tctacaaaga
     1081 caacaaccag gaccttccgc ttcctctgcc ggatggaacc attcgaatct ggaagggcaa
     1141 agaatttcgc gctgagctac tcaagtggat gggaagcatc aacgaattga agttaggcgg
     1201 cattcccaac atgctttcga tccagttgcc tccggaaaaa ctcaaaggag atgcagcaaa
     1261 catggcagcc atccacctga ctggatactc tccttcgcgt actcagattg ctttttatcg
     1321 ccttcttaca aaatccctgg cagaaaagag aattcaaaca gttgtttact ggccagtggt
     1381 atcggatgcg ttccaggaaa aaatagacga cgcacgactt ccggattttg tgcaaagggc
     1441 gcgagtggaa caggaaatca aaaaagtgga tccggcagga atcatccagt tgatctatcc
     1501 tgcagaattg aaatgccgca cattcgtcga ttcagttcat ctaagcggct catgctttcc
     1561 tgaactcgaa aaaatactga tcgagaaggg acttccggct caaaagcgga agtaaatgaa
     1621 ctgcactgtg ctgcggtcca gtcgcaccag catatagaac agggcgactg cgatgacggg
     1681 aatcaaatag cgtccgtatt tgagaagtgg tgcggccaat ctttcacgct ggtattgcac
     1741 aacgtgaaca agaatggaaa gagcaacaag tcctggaaac ctggtccagt taggcggcat
     1801 cacagccccg gccgcagtat ccccgctgag agtgtgtatc atggccatgg cacttgtgaa
     1861 atcatgcccg cggaaaaaga gagcagaaga actccaaaga gtaaacatca caacccagag
     1921 agctgccttc gccgggaaag aaaattccct ctttcctatt ccaagcaatc tctcgatagt
     1981 catgagcgcg ccaatgtaga cgccccagaa aaagaacatg tagctatgac cgtgccagag
     2041 tccggccaga atcatggtga tcaaaacatt caaatgactt cgcagtggac tcgtgcgaga
     2101 tccaccgagc gggaaaaaga tataatcccg caaccacata gacagtgtga tgtgccactt
     2161 gcgccagaga tcggcgaacg tgtaagaaaa gtaaggggcg cgaaagttct caggaatttc
     2221 gtaacccagc atcttggcca gaccacgggc tatgtctgta tatcctgaaa aatctgcata
     2281 aatctggagt gcaaaaccaa agagcgccgc aaccgcaccc acagtcgtga acttcgcggg
     2341 attggaataa acaggatcaa tgatggaact gattccgtct gcgatgacta ccttcttcaa
     2401 aaggccaaac aggatcaggt agattccagc cgtggactgg gtgcgatcaa atctctccgt
     2461 gccgatctgc ggaaagtaat ctctgtgcct taagatggga cctgcaatca gatgcgggaa
     2521 gaagaagctg tattgtaaga aattctgaaa accaatctcc tcgtctatat ccccgcgcca
     2581 cgcatccact agaatcgcaa tcaactgaaa ggtgtaaaag ctgatggcca gaggaaaacc
     2641 gaaatggagc gcctcgcctg catgcgaaaa tgcagccagc cctgtaactc cacccagcca
     2701 ctgcaaggtg gtggctacat acttaaagaa aacaagattc gcaatgttca gcgcaagtcc
     2761 aacgtagaac caggacttgc tgtgcttctc gcgcacgcgc aagtagcaat agtagctgat
     2821 cagaactaca gctgtgaaat gaaagagata aagaaaatcc caccaggcat aaaaggccat
     2881 ggaagaaaga atgagccacc tgcgacgcca ttcgccttgc agctgccagt agactgcata
     2941 gaatacgaag aagaaaacta agaaatcgaa agaattaaaa agcatcccta gtccagccgg
     3001 cgaatctgga tgctcgtaaa ccgttcgctg ccagcggcaa catccgagac cacgacaatt
     3061 tcatctccgg tcaaaacacg attgcgagcc ctgagcttct caaacgcggc acggatattc
     3121 ttttcaggat cgtttgagaa gtccataaga aaaggcacca cacctcgagc gagccagagc
     3181 tttcgcctgg agctggacat attcgtaaaa gcgtaaatga tagaggtact tggtctgaaa
     3241 ctcgccacct gttctgcgag gattccacga cgagtcatca caacaatggc ggggcttttc
     3301 agcgaatcgg cgagcttgca cgcgcttctt gcaagctgct cgcgaaagct acctgcaggc
     3361 cgggccagtg caaacccaat tcctggttct ttctcaagtc ttcgcgcaat ttgatccagc
     3421 acttcgatgc atttcacggg atactttcca accgccgttt ctccagaaag catcagcgcg
     3481 tccgcttgct cgaagactac attggcaaca tccgttgcct ctgcgcgagt aggcattgga
     3541 ttttgtatca tgctctcaag caaatgcgtt gcgacgatga ctggcttgcc cgcgtcacaa
     3601 catttgcgca ccatcattcg ctgtatgact
//
LOCUS       DAOQJL010000407         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16136_length_3624_cov_4.766881, whole genome shotgun sequence.
ACCESSION   DAOQJL010000407 DAOQJL010000000
VERSION     DAOQJL010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16136_length_3624_cov_4.766881"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1253
                     /locus_tag="PLG78_15155"
     CDS             24..1253
                     /locus_tag="PLG78_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE24511.1"
                     /translation="MGVLRLGLAFEESSLKGASFKAKDYFIFTFDHVPLSEQDAGIRF
                     KVPEEIRVEGGPFQLLPTVISVRVSPDSPYVVKGTPEGPALFLGEERIGSVGFPPVPA
                     WYDRKTASGKLPGEVAPVIEWGYLIYLTAFRNCQYFGKDEECAYCDINHNYRQQKGEG
                     RPYTGVKDVSDILEVLSWIAETDTNRIAKAYTITGGSVTTQLRGKGEVDFYLEYASAI
                     ESKFPGRWIGKIVTQAWEKEEVRKFKDAGIRIYHPNYEVWDPELFKKICPGKERTIGR
                     DTWIRRILESTEVMGPENVIPNFVGGVELAKPYGFSTVDEAIQSTAEGLDFFMSHGVA
                     PRFTTWCPEPFTTLSTLSDQTPPPLEYFCKLLTVWKETHEKYKLPVPPGYGDPGPGRA
                     VFSVSAFMDIIGYEGRN"
     gene            complement(1299..1682)
                     /locus_tag="PLG78_15160"
     CDS             complement(1299..1682)
                     /locus_tag="PLG78_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24512.1"
                     /translation="MKPTWKKILAVLMERFPEIESVVFARVKPGALERSAFSPFLLMA
                     FYLDEYSPVRRLRELELALIRLFYLEEEKIRFAAGRGSSQALFPITELMERVREKGAL
                     VYKRARAPRISARELLAIQLMNSAA"
     gene            complement(1780..2844)
                     /gene="dusA"
                     /locus_tag="PLG78_15165"
     CDS             complement(1780..2844)
                     /gene="dusA"
                     /locus_tag="PLG78_15165"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNE24513.1"
                     /translation="MDRQSLSSAASTKISDRRISLAPMMEVTDRHFRRMLRFLSRDAH
                     LYTEMVHSRAVLADARRYLAHMSAEYPLVLQLGGDDPMELEEASRIAVDMGYSEINLN
                     CGCPSERVQRGGFGACLMAEPVLVSQIVSAMKKSGASITVKHRIGIQNIRTDEEPDYD
                     ALRLFVGALADAGCERFIVHARTAILTGLSPAENRSVPPLRFDLARRLKNDFPHLPFE
                     INGGIADLETAGALLEEFDGVMIGRAVRDNPMLFSGVDRVLSAVRQSPENRAELTPHD
                     LLHAMIPYVERSPDSAHIVARNLLNIFHGYPGARRYRRFLSEELPDTDHKSVADLLVE
                     ATRYIEKKAERSEEPVPSSV"
     gene            2871..3605
                     /locus_tag="PLG78_15170"
     CDS             2871..3605
                     /locus_tag="PLG78_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE24514.1"
                     /translation="MKKTVSIALISMLGLTQITCAGMSSKVPIAMAIGCASGFGLGAI
                     YDEVARNKDAKEKKSVQDRALGIFKNKKKTYNQGKFVGLGVGCLAGLGTGLYLDMMAE
                     DMQQQMNARGIQLEKVDSNGNGETDELLVKMDGNISFATNDSVLQGVAKTNVENLAEG
                     LRGYPETKIRIGGHSDGTGTLAVNQPLSQKRAESVRDALVGKGLESGRVAEVAGFANS
                     KPLPGTNPSGNEPNNRRVEVRIVAAE"
BASE COUNT          958 a          927 c          916 g          823 t
ORIGIN      
        1 gagtacttgc cgtcgtcctt gaaatcggtg tactgcggct tggtctcgcc tttgaagagt
       61 cgtctctcaa gggagcttcc ttcaaagcta aagattattt catattcaca ttcgaccatg
      121 tacctctgtc agagcaggac gccggaattc gtttcaaggt tcccgaggaa atacgagtcg
      181 agggaggacc gtttcagctt ctacccactg tgatctccgt tcgagtcagc ccggactctc
      241 cgtatgttgt gaaaggcact ccggaaggac ccgcactctt cctcggggaa gaaagaatcg
      301 gctctgttgg atttcctcct gtcccggcct ggtacgatcg aaagactgcg tccggcaaac
      361 ttcccggaga agtggctcca gtcattgaat ggggctacct gatttacctc actgcctttc
      421 gcaattgcca gtactttggc aaagacgaag agtgcgcgta ttgcgacatc aaccacaact
      481 atcgtcagca aaaaggagaa ggcagacctt acacgggagt aaaagacgta tcagatattc
      541 tggaagtact ttcgtggatt gctgaaacag atacgaatcg catagcgaag gcttacacga
      601 tcacaggcgg aagtgtcacc actcaattgc gagggaaagg agaagttgat ttttacctgg
      661 aatacgcatc cgcgattgaa tccaaattcc ccggcagatg gatcggaaag attgtaacgc
      721 aggcctggga gaaggaagaa gttcgcaaat tcaaggacgc aggaatcaga atctaccacc
      781 cgaactacga ggtatgggat cctgaactgt ttaagaagat ttgtccgggc aaggaaagaa
      841 cgattggccg cgacacatgg atccgacgca ttctagagtc cacggaagtg atgggccctg
      901 aaaatgtgat tccaaacttt gtgggcggag tggaacttgc aaaaccttat ggtttctcaa
      961 ctgttgatga agccatacaa tctactgcag agggacttga tttcttcatg agccatggcg
     1021 ttgcaccaag attcaccacc tggtgtccgg aaccattcac aactctgtcc actctctcgg
     1081 accagacccc tccgcctctc gaatactttt gcaagctatt gacggtatgg aaagagacgc
     1141 acgaaaaata caagctgccc gtccctcctg gctatggcga tccaggccct gggcgcgctg
     1201 tcttctcagt cagcgcgttt atggacatca ttggatatga gggccgaaac tgatttcgaa
     1261 atgttgaaat caagaaacgg aaattcaatc ggcgggattc aggcagcaga gttcatcagt
     1321 tgaattgcaa ggagttccct cgctgaaatg cgcggagcgc gcgccctctt gtaaaccaga
     1381 gccccttttt cacgaactct ctccatgagt tctgtgatgg gaaaaagtgc ctgggaagaa
     1441 cccctccccg ccgcgaatct gattttctct tcttccagat agaaaaggcg aatgagagcg
     1501 agttccagtt ctctgagcct gcgaacgggc gaatattcat cgagatagaa tgccatcagc
     1561 aagaaaggag aaaaagcaga cctctcaagg gcccctggct taacacgtgc aaacacaaca
     1621 ctttcaatct ccggaaagcg ctccatgaga actgccagga tttttttcca tgttggcttc
     1681 atctctgctc gaaacacaca acggtgccca ctgcccggat ttctccctct taattctggt
     1741 atatcaagtg cattcatttc ttcctccgcg caggttgcgc tatactgagg aaggtactgg
     1801 ctcttcggat cgttccgctt ttttctctat ataacgcgtt gcttccacaa gcaaatcggc
     1861 cactgattta tgatccgtat ccggaagctc ttcactcagg aaacgacgat accgacgtgc
     1921 ccccgggtaa ccatggaaga tatttaacaa attgcgagcc acaatgtgag cggagtctgg
     1981 agatctttct acgtagggga tcatcgcatg cagcagatca tgcggcgtga gttctgcgcg
     2041 attctcagga ctctggcgaa ccgcagagag aacccgatcg actcctgaaa aaagcattgg
     2101 attatctctc accgcgcgac caatcatcac gccgtcgaac tcttctaaaa gggcgcctgc
     2161 agtttccaga tctgcgatgc ctccgttgat ttcaaatgga agatggggaa aatcattttt
     2221 cagtcttcgc gcgagatcga aacgaagagg aggcacagac cgattctccg ccggagaaag
     2281 ccctgtcagg atcgcagtgc gtgcatgcac gatgaatcgt tcacatcctg catctgcaag
     2341 cgctccgaca aacaaccgca aggcgtcata gtccggctct tcgtctgtgc gaatgttttg
     2401 aattccaatc ctgtgcttca ctgtaatgga agcgcctgac ttcttcatgg cagacacaat
     2461 ctgcgaaaca aggactggct ctgccatcag gcaggcgcca aatccgccgc gctgcacgcg
     2521 ctcactcgga catccgcaat tcaaattgat ttcagaatag cccatatcca ctgctatgcg
     2581 agacgcctct tcaagctcca tcggatcgtc tccaccgagc tgaagcacga gagggtattc
     2641 tgcactcata tgcgcgaggt agcgccgggc gtctgcgagg accgccctgc tatgaaccat
     2701 ttcagtgtag agatgagcat ccctggacag aaaccgaagc attctgcgga aatgcctgtc
     2761 ggtcacctcc atcatggggg ccagactgat tctacggtcg cttattttgg ttgatgcagc
     2821 tgaactcaag gattgtctgt ccacccggcc cttttaatgg aggaaatcaa atgaaaaaaa
     2881 cggtgagcat tgcactcatc tctatgcttg gcctgacgca gatcacatgc gctggcatga
     2941 gcagcaaagt accaatcgcc atggcgattg gatgtgcgtc cggtttcggt cttggcgcca
     3001 tctacgatga agttgcgcgt aacaaagacg cgaaagaaaa gaaaagcgta caggatcgcg
     3061 ctcttggtat cttcaaaaac aaaaagaaaa catacaatca aggcaagttc gttggcctcg
     3121 gggtaggatg tctggcaggt ctgggcaccg gtctgtatct ggacatgatg gcggaagata
     3181 tgcagcagca aatgaatgcg cgcggcatcc agcttgaaaa agtcgattca aacggaaacg
     3241 gcgaaacaga tgagctcctt gtgaaaatgg atggaaacat ttcttttgcc acaaacgatt
     3301 ccgttctcca gggtgttgcg aagacaaacg tagaaaacct tgcggaaggt cttcgcggtt
     3361 acccggaaac aaaaatcaga atcggcggtc attctgacgg aaccggcaca cttgcggtca
     3421 accagcctct cagccagaag agagcagaat ccgttcgcga cgcgctcgtg ggcaaaggtc
     3481 ttgaaagcgg tcgagtggca gaggtggctg ggtttgcaaa ttcaaaacca cttccaggaa
     3541 cgaacccgtc aggcaatgaa cccaacaatc gccgcgtaga agttcgcatc gtcgcagcag
     3601 aataagttag aaattttcaa caag
//
LOCUS       DAOQJL010000408         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16169_length_3620_cov_4.333520, whole genome shotgun sequence.
ACCESSION   DAOQJL010000408 DAOQJL010000000
VERSION     DAOQJL010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16169_length_3620_cov_4.333520"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLG78_15175"
     CDS             <1..288
                     /locus_tag="PLG78_15175"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004505524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNE24515.1"
                     /translation="EAAGLYPYEQVSVVNINNGSRLETYIIEGERGSGMVCLNGAAAR
                     MAAKGDLVIVMAYGFFQPGEIAPDYKANVVHVDTQNRITSVEHGELHGQVR"
     gene            288..1001
                     /locus_tag="PLG78_15180"
     CDS             288..1001
                     /locus_tag="PLG78_15180"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNE24516.1"
                     /translation="MLSRLAAGFRAYFSSLGFLRKNNLLSHIFLPALAGLLVVALLGA
                     CVIFFSGGLSESIFLRLEQFHDLPDFVRTSLRLLFIFAGLVLTVIAYRPLSSMLVLPF
                     IGPILAQVEKVLLGKEIETTVSREIKNAMIGGWLGVQASIAALFIFVITLPLGPVQAP
                     VMFLVNSYILGKSAFDFIFEKETGSVAERRAITKKYRPEILGVGIGFILFLLVPIAGA
                     ALAPIFAVVAAARIRHGSN"
     gene            998..3127
                     /locus_tag="PLG78_15185"
     CDS             998..3127
                     /locus_tag="PLG78_15185"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE24517.1"
                     /translation="MNAETLALLPAIAAVISVINGTYAFRRRSVRGALYFFLMPLGTA
                     IWSFGYAGELLGKTQPFMTAMDNLEFIGSDLVVIGFFLFAWHYTRTTRPPLFLLAFPV
                     INQIIAWTDTGLLRHGTQVKEMGGVQFLVYDYGPYMWANIAVYYSLLLAGSFLFARRL
                     PSVHGIFRTQIVLMILSATIPWIASLFTVFGLLPIPIPNLDISPASLTVGNLLLTAAL
                     FSFGTLRISPLARERTFEMIEEGVLVCDQHGVIVDFNSSAKRLLGDKYSLEIGQRIAD
                     ELARAPSEYSVSDSSWLQVRRNSLLDSSGTEIGKIYTLQDVTQHRQARRALQDATTQA
                     QNANKAKSIFLANVSHEIRTPLTAILGIAEILEGTKLDEEQKRMVSSFRSAGTSLLET
                     INGLLDLSRIEAGKMQMEARVFSLHEMLENTCRMFRLQIESREEDARVELKLTIAPGV
                     PENIKADDLRLGQILTNIIGNAVKFTRVGKIDVRAVFHQGILQIVVQDTGIGIPKKKI
                     DNIFKPFEQGDVSTTRSFGGTGLGLAIVRELVRLMQGMISVHSVPGQGSVFSVTLPVE
                     VVEGEKKAEKKDTPSWTGSILVAEDNEVIRLILEKHLTGLGMKPTLAENGQEAVQLYN
                     TNAFDAGIFDIQMPVMDGFEAARSIRALEKAQGKRRMPLLALSASAMPEDVQNARDAG
                     FDAHIAKPVTRDQLLNALAKCALRETQ"
     gene            3143..>3620
                     /locus_tag="PLG78_15190"
     CDS             3143..>3620
                     /locus_tag="PLG78_15190"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNE24518.1"
                     /translation="MGWISAAAGLALVFIILWDAFEAMVLPRRVTQKIRLTRLFYAIA
                     WRPWAALARHVKKPGRRENYLSFFGPLSLLILIGIWATGLLLGFALLHFGIGKLRPDA
                     SDFISTLYMSGTTLFTLGIGDVTPESGTSKMLTVLESGTGIAFLALIISYLPVLYGA"
BASE COUNT          841 a          933 c          932 g          914 t
ORIGIN      
        1 gaagcggcag gcttgtaccc atatgaacag gtttccgtcg tgaacatcaa caacggatcg
       61 agactggaaa cctacatcat tgaaggcgag cgaggcagcg gcatggtctg cctcaacgga
      121 gccgccgctc gcatggcagc gaaaggagac cttgtcattg taatggcata tggttttttc
      181 cagccgggag aaatcgctcc cgactacaaa gcgaatgtgg tccacgtaga cacacaaaat
      241 agaatcacca gtgtggagca cggggagttg cacggccagg tacgctgatg ctttctcgat
      301 tggcggccgg ttttcgggcc tacttctcaa gcctgggttt tcttagaaaa aacaatctgc
      361 tttcgcacat atttttacct gctcttgcgg gcttgctcgt cgttgcactg ttaggcgctt
      421 gcgtgatttt cttttccggt ggactatcag agagcatttt cctgcgactg gaacagtttc
      481 acgacttgcc tgattttgtt cgcacttctt taagactgct cttcatcttt gcgggtctag
      541 tcttgacggt gatcgcctat cgtcctttgt cctcgatgct tgttcttcct ttcattggac
      601 cgatccttgc tcaggtcgaa aaagttttgc ttggaaaaga aattgaaaca accgtgagca
      661 gggaaatcaa aaacgcgatg attggtggct ggcttggtgt tcaggcatcc atcgctgcac
      721 tgtttatttt cgtaattacg cttcctcttg gtcctgtcca ggcgccggtg atgtttctcg
      781 tgaactctta catcctggga aaaagtgcgt tcgacttcat ttttgaaaaa gaaactggtt
      841 cagtcgcgga gcgacgcgcc atcacaaaga aatatcgacc ggaaatactt ggtgtaggga
      901 taggattcat tctgtttctg cttgttccaa ttgcgggagc ggcgctggca cccattttcg
      961 ctgtggtggc agctgctcga attcggcacg gctcaaattg aatgcagaaa ccctggccct
     1021 cttgcccgct atcgcggctg taatctcagt catcaacgga acgtacgcat ttaggcgcag
     1081 gtcagttcga ggtgctcttt atttttttct gatgccgctt ggcacggcga tctggtcctt
     1141 tggatacgcc ggcgaattac ttggaaaaac tcaacctttt atgacggcga tggacaacct
     1201 ggagttcatc ggctctgacc tggtggtcat tggatttttt ctcttcgcgt ggcactacac
     1261 caggaccact cgtcctcccc tcttcttact cgcttttcct gtcatcaacc agatcatcgc
     1321 gtggacggat acgggactgc tccgccatgg aacacaggtc aaagagatgg gaggcgttca
     1381 gttcctggtc tacgactacg gcccctacat gtgggccaac atagcagtct actattcgct
     1441 cctgcttgca ggaagtttct tgtttgcgcg aagactgcct tccgttcacg ggatctttcg
     1501 cactcaaatc gttctgatga tcctcagcgc gaccattccc tggatcgcct ccctgttcac
     1561 agttttcgga ctgctgccca ttcccattcc aaatctcgat atttccccgg cttccctcac
     1621 ggtaggcaat ttacttctca ccgcggccct tttttccttc gggactctgc gaattagccc
     1681 ccttgcgcga gagcgcacat ttgaaatgat agaagaaggc gtgctggtgt gcgatcaaca
     1741 tggagttatt gtagatttca actcctccgc caaacgcctg ctaggcgata aatacagtct
     1801 ggaaataggc cagaggatcg cagatgaact ggcaagagct ccttcagaat actccgtgtc
     1861 cgactcgtca tggctccagg ttcgcagaaa ttctcttctg gactccagcg gaaccgaaat
     1921 cggaaagatc tataccctgc aagatgtaac gcagcatcgc caggcaagga gagcgctcca
     1981 ggatgctacc acacaggcac agaatgcaaa taaggcgaag agcatttttc tggccaacgt
     2041 aagtcatgaa attcgcacgc ccctcacagc gattctggga attgctgaga tcctggaagg
     2101 cacgaaactg gacgaagagc agaaacgtat ggttagctct tttcgctcgg caggaacgag
     2161 tctgctcgag acgatcaacg gccttctaga tttgtccaga attgaagcgg gcaaaatgca
     2221 aatggaggct cgcgtattca gccttcatga aatgctcgaa aacacatgca gaatgtttcg
     2281 cctccagata gaatcccgcg aagaggacgc aagagtagaa ctgaagttga cgatcgctcc
     2341 gggtgtacct gagaacataa aggcagacga tcttcgcctg gggcagatcc tcaccaacat
     2401 cattggcaat gcggtgaaat ttactcgcgt cgggaaaatt gacgtccgcg ctgtgttcca
     2461 tcaagggata ctgcaaattg tagtgcagga taccggcatc gggattccga agaagaagat
     2521 tgataatatt tttaaaccat ttgaacaggg cgatgtatcc acgacgcgct cctttggcgg
     2581 aacgggcctg ggccttgcca ttgtccgcga gctggtgcga ctgatgcagg gaatgatctc
     2641 cgtgcacagc gttccgggcc aggggagcgt gttctccgtt acacttccag tagaagtagt
     2701 agagggagaa aagaaagcgg agaagaagga cactcccagc tggactggat cgatcctggt
     2761 tgcggaagac aatgaagtca tccgtctgat tcttgaaaag cacctgaccg gtcttggtat
     2821 gaaaccaacg ctcgccgaaa atggacagga agcagtgcaa ctctataata cgaacgcatt
     2881 cgacgcaggg atctttgaca tccagatgcc tgtgatggat ggatttgaag cagcacgctc
     2941 cattcgagct cttgaaaaag cgcagggtaa acgcagaatg cctcttctgg cgctctccgc
     3001 atctgccatg ccggaagatg ttcaaaatgc tcgcgacgca ggattcgacg ctcacatcgc
     3061 caaacctgta acacgcgacc agcttttgaa cgcacttgcg aagtgcgctt tacgcgagac
     3121 gcagtagtcg cgtcttcagt ctatgggatg gatctcagcg gctgcaggcc tggcacttgt
     3181 ttttattata ctctgggatg cattcgaggc aatggtactg ccacggcgcg tgactcagaa
     3241 gattcgccta acgcgtctgt tctacgcaat tgcctggcgc ccctgggccg cactggcccg
     3301 acacgtgaag aagcctggca ggcgcgaaaa ctacctgagt ttctttggtc cgctctcttt
     3361 gctcattctc attggaatct gggccaccgg tctcctcctc gggtttgcac ttctccattt
     3421 tggaatagga aagctcagac cggacgcctc cgatttcata agcacacttt acatgagcgg
     3481 aacgactctg ttcactcttg gcatcgggga tgtaacaccg gaatccggca catccaaaat
     3541 gctgacggta ttagaatcgg ggacgggaat cgcgtttctt gctcttatta tcagttacct
     3601 gcctgtgctt tatggtgcat
//
LOCUS       DAOQJL010000409         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16185_length_3619_cov_4.634119, whole genome shotgun sequence.
ACCESSION   DAOQJL010000409 DAOQJL010000000
VERSION     DAOQJL010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16185_length_3619_cov_4.634119"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..510
                     /locus_tag="PLG78_15195"
     CDS             130..510
                     /locus_tag="PLG78_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24519.1"
                     /translation="MNRKRIAIAAIAAALFLGAASYLSAESDYDFTLVNATGYDIDAV
                     YVSPSSQESWGNDVMGQDVLVNGMTVKIVFHPSAQASSWDLKVKWRDPGYPAVVWHGL
                     DLATINKLTLKYNKDTDVTSVIKE"
     gene            complement(554..1210)
                     /locus_tag="PLG78_15200"
     CDS             complement(554..1210)
                     /locus_tag="PLG78_15200"
                     /inference="COORDINATES: protein motif:HMM:NF039832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HNE24520.1"
                     /translation="MKSRIKNRPEFKPDMGVRQFQAQYWYREELIQICRAHGLDYSGA
                     KADLEERLIGFLRGPVSPPIARQKKKTEKRKYPVSGTSREVVTRRISLDSPVSSGFAF
                     NAHAREFFAEYFKNPDFKFTMEMAEFVRDARAKGNERAKVQDLVKIYERVQREKKDGT
                     RKLPPILRWNTFVRDFNRDPKTAHLKNKMEAAAKLWGIVRDRPGPKKYDPSLLKFLKE
                     "
     gene            complement(1216..1905)
                     /locus_tag="PLG78_15205"
     CDS             complement(1216..1905)
                     /locus_tag="PLG78_15205"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HNE24521.1"
                     /translation="MAAKKKAQKKIQNKAQKKSGKKTRAPLKKSIQASALPPMKPAVF
                     PLEKLGPLRPLVGVWEGQKGDDIAPDDSREGIENNKFRERIEFVPIIPVDNHEQKLFG
                     LRYKTTVWRLTESDPFHEEVGYWLWDPKEKQVMKSFIVPRGIVVLAGGTVEPSAQEFN
                     LSADAGSSTYGICSNLFLDREFKTVRYELNVKLIAGEFRYFEDTQMKMPGRADLFHHT
                     DENALRRSKAL"
     gene            complement(1915..2958)
                     /gene="selD"
                     /locus_tag="PLG78_15210"
     CDS             complement(1915..2958)
                     /gene="selD"
                     /locus_tag="PLG78_15210"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNE24522.1"
                     /translation="MNADSIRLTTYSHGAGCGCKISPRVLDEILKGNWEKPNHDRLIV
                     GNDEKDDAAVYDLGGGNGLISTTDFFMPIVDDPFDFGRIASANAISDVYAMGGRPVLA
                     LAILGWPVDKIPGEVAGRVIAGARSLCAEAGVMIGGGHSIDSPEPIFGLAVNGLVRLD
                     RLKRNSTARPGDLLFLTKPLGIGILTTAEKKGMLRTSDAGIASSVMLRLNRIGEELSE
                     IEGVHAMTDVTGFGLLGHLSGMCSASGTSAVIYSDRVRRITDLDYYLEKGCVPGGTKR
                     NFDSYGSGISAMQESVKNVLCDPQTSGGILVSVNPDSADQVRALFSKNDLAEFKEPVG
                     EIISQGAELIRVQ"
     gene            complement(2955..>3619)
                     /locus_tag="PLG78_15215"
     CDS             complement(2955..>3619)
                     /locus_tag="PLG78_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24523.1"
                     /translation="RRFVVVGGKTGAGKTKVLHQLCALGQPVIDLEYLAGHKGSAFGG
                     LGQKDKTQEQFENDLCISLRSLDPLCETDGGIHSGSIAWIEDESRTIGKRSVPAQIMQ
                     SKQEATLLFLDVPLKQRVENLVREYGQFPVQDLANIISRLHKRLGGLDTAQALEALHA
                     GDLERVCEIVLHYYDRAYDFELSKRSGPVIRVPVSSMDAAENASRLMQACKESVRTEA
                     MT"
BASE COUNT          835 a         1025 c          807 g          952 t
ORIGIN      
        1 atacgacgct ccatctcaaa aataaaaaaa ttcacgtttc gcacgattcg ttcgtcctat
       61 aattgttgcc ggcgaaacgc aggcaattcc aataagccgg gcctcggccc actatcttga
      121 gaggaaaaaa tgaatagaaa gagaatcgca atcgcagcaa ttgcagctgc actgtttctc
      181 ggcgcggcaa gctacctttc cgccgaatca gattacgact ttacgctcgt aaacgcaact
      241 ggctacgaca tcgatgcagt gtatgtatcg ccatccagcc aggagagctg gggaaatgac
      301 gtaatgggtc aggacgttct tgtaaacggc atgactgtaa aaatcgtatt ccatccatct
      361 gctcaggcat cttcctggga tctgaaagta aaatggcgtg atccaggata tcctgctgta
      421 gtatggcatg gcctggacct ggcaaccatc aacaagctga ctctgaagta caacaaagac
      481 acagacgtta cttccgtaat caaagagtaa tctttgcatt gcatcccggg gcattgcccc
      541 ggggtgaatg cctctactct ttcagaaact tcaaaagaga cggatcatac ttctttggtc
      601 ccggtctatc tctcacaatt ccccacagtt tagccgcagc ttccattttg tttttcaagt
      661 gtgccgtttt cggatcgcgg ttaaaatcgc gtacaaaggt attccagcgc aaaatcggcg
      721 gaagctttcg cgtaccatct ttcttctccc gctgcaccct ctcgtaaatc ttcaccagat
      781 cctggacttt cgcacgctcg tttcccttcg cacgcgcatc gcgcacaaac tctgccatct
      841 ccatagtaaa cttgaaatcc ggattcttga agtattcggc aaagaactct ctggcatgcg
      901 cgttgaaagc aaagccggag gatactggtg aatcaagaga gatacgcctg gtgacaacct
      961 ctctcgatgt gcccgagacc ggatactttc gcttttccgt ctttttcttt tgtctggcga
     1021 tgggcggcga gaccgggccg cgcagaaaac caatcaacct ctcttctaga tctgccttgg
     1081 ctccactgta gtccagtccg tgcgcgcgac aaatttgaat caattcttcc cgataccagt
     1141 attgcgcctg aaactggcgc actcccatat ctggtttaaa ctcagggcga ttcttgattc
     1201 tggacttcat ccggtctaga gtgctttgct gcgcctcagt gcgttttcat ccgtatgatg
     1261 aaatagatcc gctctccccg gcatcttcat ctgcgtgtct tcgaaatacc tgaattctcc
     1321 ggcaatgagt ttcacattga gttcatagcg aactgtcttg aattcgcggt ctaaaaacaa
     1381 attggaacag atcccgtagg tagaagatcc agcgtccgcg gacaaattga attcctgagc
     1441 agaaggctcc accgttccac ccgccagaac tacgattcca cggggcacaa tgaagctttt
     1501 catcacttgc ttttctttag gatcccaaag ccagtaacct acttcttcgt gaaatggatc
     1561 tgattcagta agcctccaga cggttgtttt gtagcgcagg ccgaatagct tctgttcgtg
     1621 attgtctacc gggatgatgg gtacaaactc gattcgctct ctaaatttgt tgttttcaat
     1681 tccctcgcgc gaatcgtccg gagcaatgtc atctcccttc tggccttccc acactcccac
     1741 cagtggtctc agcggaccaa gcttttccag tggaaagacc gcaggcttca tgggcggcag
     1801 agcgcttgcc tgaatggact ttttcaaagg ggcgcgcgtc tttttcccgc tctttttctg
     1861 ggctttgttc tggatctttt tttgcgcctt cttctttgcc gccattccat cttcctactg
     1921 gacgcgaatc aattccgcgc cctgacttat gatttcaccc actggctctt tgaattccgc
     1981 cagatcattt ttcgaaaaca atgcgcgcac ctggtctgcg gagtccgggt tcacggaaac
     2041 cagaatgccg cctgacgtct gcggatcgca gagcacgttt ttcacggatt cttgcatggc
     2101 agaaattccg cttccataac tgtcaaaatt tcgtttggtt ccaccgggca cacaaccttt
     2161 ctccaggtag tagtccagat ccgtgatcct tctgactcga tccgagtaga tcaccgcgga
     2221 tgttcctgac gcagaacaca tgccactcag atgacccagt agaccaaatc ctgtcacgtc
     2281 cgtcatcgca tggacacctt ctatctccga aagctcttct ccaatgcgat tcaatcgaag
     2341 catcacactc gaagcaatcc ctgcatccga tgtgcgaagc atcccttttt tttcagcagt
     2401 ggtcagtatt ccaatcccca gaggcttggt taagaacaac agatcccccg gacgagcggt
     2461 gctatttctt ttcaatcgat ccaggcgaac gagcccattg actgcaagac cgaagattgg
     2521 ctccgggcta tcgatgctgt gcccgccccc tatcatcact ccagcttccg cgcaaaggga
     2581 tcttgcgccc gcaatcactc ttcccgccac ttctccagga atcttatcta cgggccagcc
     2641 cagaatcgcg agtgctagaa ccggacgacc tcccatcgca tagacgtcgc tgatcgcatt
     2701 ggcggatgca attcttccaa aatcaaaagg atcgtccacg atcggcatga agaaatctgt
     2761 agtactgatg agtccatttc caccgcccag gtcatagact gcagcatcat ctttctcatc
     2821 attgcctaca atcaagcgat catggttggg tttctcccag ttgcctttga gaatctcatc
     2881 cagaacgcga ggcgaaattt tgcaaccgca gcctgccccg tggctgtatg tagtgaggcg
     2941 aatcgaatct gcgttcatgt cattgcttct gtgcgaacag attccttgca ggcctgcatc
     3001 aacctggatg cgttttctgc cgcgtccatg gaagacaccg ggactcgaat cacagggcca
     3061 cttctttttg aaagctcaaa atcataggct cgatcatagt aatgcaaaac gatttcgcac
     3121 actcgttcta gatctcctgc gtgcaacgcc tcaagcgcct gcgctgtgtc gaggccaccg
     3181 agtcgcttat gcagcctgga gataatgttc gcaagatctt gcactggaaa ttggccgtac
     3241 tctcgcacca ggttctccac tctttgcttg agtggaacat ccagaaacaa aagagtcgct
     3301 tcctgcttgg attgcatgat ttgcgcgggc acgcttcgct ttccaatcgt cctgctttcg
     3361 tcttcgatcc aggcgatcga tccgctgtga atgcccccgt cagtttcgca caacggatcg
     3421 agcgatcgca aagagatgca tagatcattt tcgaactgtt cttgcgtctt gtccttttgt
     3481 ccaagcccgc cgaaagcgga tcccttgtgg cccgccagat attccagatc gatgacgggc
     3541 tgtccaagtg cacacaactg atgcaaaact tttgttttgc ccgcgcctgt tttcccgcct
     3601 acgactacaa agcgtcttg
//
LOCUS       DAOQJL010000410         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16240_length_3612_cov_4.602755, whole genome shotgun sequence.
ACCESSION   DAOQJL010000410 DAOQJL010000000
VERSION     DAOQJL010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16240_length_3612_cov_4.602755"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1342)
                     /locus_tag="PLG78_15220"
     CDS             complement(179..1342)
                     /locus_tag="PLG78_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24524.1"
                     /translation="MRAFLAILVLGFVNCAKYDAPAGIFGYLLITAAANNHGPCYNPT
                     VKVQTGSTTSSANGTTTVTISAIDTSTSFLIFQATSAGDRPPGATIGGTIASSTTVDF
                     ERFTDGLTTEPAVITIQYYVVTYPSGCASVQRGITNLNADPKNISISSVSSISQAMIL
                     FSRSTGTTENVYDSNNITTAYLTSTSNLRIACAGPSNTNAYWQVVDFPYASIRNQSGS
                     TSILGAATSASVPISSVDLSRSIVLTSLYDSGATGNIGANMVRAYLPDSTTLLIERDD
                     VGAPDNLTRIDYQVIEFQDGTTVQRGNLAFSGGASNATLSLANAVDTSASAPFASFQF
                     GPGQSSGKTPYTANDLVGMATATMTLSSTTLSATRGVTGATADIYAYAVQFGK"
     gene            complement(1377..2333)
                     /locus_tag="PLG78_15225"
     CDS             complement(1377..2333)
                     /locus_tag="PLG78_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24525.1"
                     /translation="MGLPGRIVLFVFLAAAAPAGAAPLRISYNLQSTDPTEDQLAALT
                     SYIRDNKGSYFLLQGFACDTGGFRVSLKIASQRQDKIQALIQKEVGRDHTALANPAVI
                     EGEPRIEHRKLEIEAFKTPEALAAAVRLANENTARFHSAFQVETPPETHNEPEDGHPL
                     LWLGLLLAALVIVFIYFILTGNERKRRRHALTPEQAEAVQAITGEAPVQPSQKTRKHR
                     IVTAESVIEPAAFVKELKPMASKKKASKPGPTIKKALDKAYENRSLGEIAKSPVHSLQ
                     GLTPRHSKMLEEAFGVKTVEDLAGLKYFELARAITVLARYED"
     gene            2389..3006
                     /locus_tag="PLG78_15230"
     CDS             2389..3006
                     /locus_tag="PLG78_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24526.1"
                     /translation="MQNIDFSRKIRREPLVESEGSTSGIKNRAVVTTFALCILAFTVG
                     IAAGVQIMRVRGTDSLVRFPDKATSHSESRSSEKSTDPEEPASERSTRVGTEPSEEPA
                     KMSDSSPSRYLIKVGTFPSADADRIARRIAKVPELKSIPPAPCKGLKEQSERGLAFRI
                     AVKGQDRENLFLGCFADPGAAHEALGQLKASGISQASSAIVFEIE"
     gene            3050..>3612
                     /locus_tag="PLG78_15235"
     CDS             3050..>3612
                     /locus_tag="PLG78_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24527.1"
                     /translation="MGLFSTLRGRLKIRVRLMILTSGIVSFSVLALSFVVIFQYQKIL
                     IEKTLDVCRNLSINVVAQAQDELLLNDDVFTSTKEAVRAVKSSGPAGFLAAYVVSQKS
                     SPRKKSPTYGRIIAHSGENHSSDEIPAQDLAYLNSINNLQYREVAGILRFAFPLFFVP
                     AHADPIRLGTVVFEFDTKVIYAPIVEVR"
BASE COUNT          788 a          893 c          975 g          956 t
ORIGIN      
        1 ctcacagaga cttcgcgcga attcctgact gactacagcg ttttcgcgta cgcgccggag
       61 agatagattc ttcagtttat gaagctggcg cggtttgaga tcggaagtgt tgcctgaaat
      121 ttgcggcatg cttgtctgat tcgaacaata taagcatgcc ccgctggatt gtcactcgtc
      181 atttgccaaa ttgaactgcg tatgcgtata tgtctgccgt ggctccagtg actcctctgg
      241 ttgcactgag cgttgtggaa ctcagtgtca tcgttgcagt tgccattcca actagatcgt
      301 ttgccgtata gggtgtttta ccagagctct gtcccggtcc aaattgaaac gatgcgaatg
      361 gagcggatgc agacgtatcc accgcatttg ccaggctcag agttgcattg cttgcgcctc
      421 cagaaaatgc gaggttgcct ctctgaacgg ttgtcccgtc ttgaaattca atcacctggt
      481 agtcaatgcg cgtcaggttg tcgggcgctc ccacatcgtc tctttcaatg agcaaggttg
      541 tggaatcggg caaataagct ctcaccatat tggcgccaat gtttccggtg gctcccgaat
      601 cgtatagact cgtaagcaca atggacctgg aaagatccac actgctgatg ggtacggaag
      661 cggacgtggc cgctccaaga atgctcgtgg atccactttg atttcgaatg cttgcatagg
      721 gaaaatcaac aacctgccag tacgcatttg tgttcgacgg gcccgcgcat gcgattcgaa
      781 gatttgaggt agatgttaga taagctgtcg tgatattgtt gctgtcgtat acgttttctg
      841 tggttccagt ggatctgctg aaaagaatca tagcctgcga aatagaggat acgcttgaga
      901 tggagatgtt ctttggatct gcgttgagat tggtaattcc tctttgtacg gacgcgcaac
      961 cggaaggata ggttactacg tagtactgaa ttgtgatgac agcgggttct gttgtaaggc
     1021 catctgtaaa gcgttcgaaa tcaacagtag tgctcgaagc gatcgtgccc cctatggtgg
     1081 cccctggagg tctgtctccg gcactggttg cctggaaaat taaaaaggat gtgctcgtgt
     1141 ctatggcaga gatggttaca gttgtggttc cgttcgcgct agaagtggtg gagcccgttt
     1201 gtactttaac agttggattg tagcaaggtc cgtgattgtt tgcagccgcc gtgatcagca
     1261 gatatccaaa aatccccgcc ggcgcgtcat acttcgcgca attgacaaag cccagaacga
     1321 gtatggcaag gaaagcgcgc acgggatatc tatgggttgc atttctagac tgtaaatcaa
     1381 tcttcgtatc gtgcaagcac tgtgatcgct cgcgccagct caaagtattt gaggcctgcc
     1441 aggtcctcta cggttttcac gccgaatgct tcctcaagca tcttgctatg tcgtggcgtg
     1501 agaccctgca gcgaatgaac ggggcttttt gcgatttcgc ccagggacct gttttcgtat
     1561 gctttatcca gcgctttctt gatcgtagga cctggtttgg atgctttctt cttggaagcc
     1621 atgggcttta actcctttac gaatgctgcg ggttcaatca cgctttctgc cgtgacgatc
     1681 cggtgctttc ttgttttttg cgaaggctgg actggagcct ctcccgtaat tgcctgaacg
     1741 gcctctgcct gttcaggcgt gagagcatgc cgccttcttt tcctttcgtt tcccgtgaga
     1801 atgaaataga taaatacaat gacaagtgct gccaggagta gacccagcca gagcagcggg
     1861 tgtccgtctt ctggctcgtt atgtgtttca ggtggagttt ctacctggaa ggctgaatgg
     1921 aatcgagctg tattttcgtt tgcaagtctt accgctgctg ccagggcctc tggcgttttg
     1981 aatgcttcga tttccagttt gcgatgttcg attcttggct ccccttctat cacagccggg
     2041 tttgccagtg cagtgtgatc gcgccctact tctttctgga tgagtgcctg gattttatcc
     2101 tggcgctggc ttgcgatctt gagcgaaacg cgaaagcctc cagtatcgca cgcaaaccct
     2161 tgtagtagaa agtaggatcc tttattgtcg cgaatgtagg acgtgagagc tgccagctgg
     2221 tcttctgttg gatctgtaga ctggaggtta taggatatac gcagcggggc cgcaccagcg
     2281 ggagccgcgg cggccaggaa aacaaagagt acgatgcggc cgggaagtcc catctttgaa
     2341 attttcggtg tgttttgtca aactgaccga tattttccat ataacgagat gcagaatatc
     2401 gatttctcac gcaagatccg ccgcgaacct ctcgtggaaa gcgaaggctc cacatcgggt
     2461 attaagaatc gagcggtcgt aaccacattt gcgctttgca ttctggcgtt cacggtgggc
     2521 attgcagccg gcgtccagat catgagagtg cggggcacag acagcctcgt gcgattccct
     2581 gacaaggcta cctcacattc cgaatcccgt agttctgaga aatcaacgga ccccgaggaa
     2641 cctgcgtccg aaaggtctac gagagtgggc actgagccat cggaagaacc ggcaaaaatg
     2701 tccgactctt ctccatcgcg ttatttgatc aaggtgggca cttttccctc tgcggacgcg
     2761 gaccgcatcg cacgtcgcat cgcaaaagtc ccggagctta agtctattcc acctgctcca
     2821 tgcaaaggac tcaaggaaca gtctgaaaga ggcctggcct ttcgcatcgc ggtgaaggga
     2881 caggacaggg agaatctgtt cctgggctgt ttcgcagacc cgggcgcggc ccatgaggcc
     2941 ctggggcagc tcaaggcgtc cggcatttcg caggcatctt ccgcgatcgt tttcgaaatc
     3001 gaataagtaa ttacttgtca gcgcccgccg ggggagtcat ggtctggcca tggggctgtt
     3061 ttcaacgctg cgcggtagac tcaagatccg cgttcgcctg atgatcctga cctccggcat
     3121 cgtaagcttc tctgttctgg cactttcctt tgtggtgatc ttccagtacc agaagatcct
     3181 gatcgaaaag acactcgatg tttgtcgcaa tctatccatc aacgttgtgg cgcaggctca
     3241 ggacgagctg ctactaaacg atgacgtatt cacttctacg aaagaagcgg ttcgtgcagt
     3301 caaaagctca gggcccgctg gatttctcgc cgcgtacgtt gtgagtcaga aaagctcgcc
     3361 caggaagaag agccccacat acggccgaat cattgctcat tcaggtgaaa atcattcatc
     3421 ggatgaaatc ccggcgcaag atctggcata cttgaattct atcaacaatc tgcaatacag
     3481 agaagtagcg ggcatccttc gatttgcatt ccctctcttt tttgttccgg cacacgcgga
     3541 tccgattcgt ctgggcacgg ttgtttttga gtttgataca aaagtaatct acgcgcccat
     3601 cgttgaggtt cg
//
LOCUS       DAOQJL010000411         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16278_length_3608_cov_4.442443, whole genome shotgun sequence.
ACCESSION   DAOQJL010000411 DAOQJL010000000
VERSION     DAOQJL010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16278_length_3608_cov_4.442443"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..73)
                     /locus_tag="PLG78_15240"
     tRNA            complement(1..73)
                     /locus_tag="PLG78_15240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Ala,seq:ggc)
     gene            161..1519
                     /locus_tag="PLG78_15245"
     CDS             161..1519
                     /locus_tag="PLG78_15245"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24528.1"
                     /translation="MELVSYILIVVLVVLAGAGAVYGYQSLRGPRKLEEIGNLIAGER
                     YREALAECQRIIQQDDRSMIAHFLAGQCHQALSEYPMAVVEYKNCIRIGKFDDQVKEI
                     PVRRGLARSLIESGNANEAKNEFLILTTLEPNQFENHFELGRLFHRGGIYPKSIKFLQ
                     TATALNVKNAEAFNVLGQSHYALKQYHDARAALIRAVQLKPDLKAAHYYLGLALRYLN
                     DLDWALKEFEIAEKDDGLRDKALLAKGMVLLDQGNFSKAITELERGLKFAPPGSETMI
                     QLYYLIGVAAEKGRDVHKAIASWEKIEQIKPGYRDVRDKLRQYQEFRTDDSVKDFLIM
                     NTTQFENACRRIIEKMGFQVLHLMLNGEMSVTGVGTDADVKSRNRGQKTLFFISRDMA
                     ALPEKIVREFHESMREKGALRGVIMTTGEVMPAALNYATTRPIEIYDSSAVVPFIRTA
                     MN"
     gene            1521..>3608
                     /locus_tag="PLG78_15250"
     CDS             1521..>3608
                     /locus_tag="PLG78_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24529.1"
                     /translation="MHHEKAGSPIVSALGNGRLQVVRNPEVDYILDLVLDEARRLVQA
                     RQGNFYLFNSISKLEPFLPAAASEQDIALCEDVIQARTSRLIPENPALCVYMTLQEGD
                     LGVLLLREPAYFDQFYEADLALVQNLAATFAILLKSAFIDQRTPEVFLGFKSSLLLLL
                     ENAHLNHKIKESDHQLKSVLEVSNLINSSRELSEMIQTVLYSAKRVIRAESASLFLVD
                     EKTGELYFEIIAGNKKDLKGFRIPRGQGIAGITAATKKSLIVNDAQNDARVFRQVDQV
                     SREITRNILAAPLLIEGEAIGVIEVINTIDRPAFSEHDLEIFESFSDSVAIALQRRRL
                     LDSIQRTNVQLERRLREITSLHAVARAQVEERTIQDLFARALNIVRDDLKVGRASILM
                     ASGSTLKMAASAGTYDESDATPGSLAHHVFSTNRPVFIEDFRENPEFAAMANPHRYDS
                     RSCIILPLASPGQPPFGVLCLAEPSSGQFLEEDYKLLLTIASQIVKGYENLGLNEQII
                     VKKSLEKEMEITSRIQRNILPIKKPQHLHLDLAARSVMAKTTGGDFFDYYVHTPNGDV
                     SCLVADVSGKSLPAALFMAVSSSILRTIIRQEIDPTRVLAQANDLLFEESQSGMFVTV
                     FLCRYDPSTGRLRYASAGHNEMILMHENGNYEMLSGKGSPLGVLPAYRQKYLGGSTQV
                     RDGDLLILYTDGVI"
BASE COUNT          977 a          914 c          892 g          825 t
ORIGIN      
        1 tggagacgag ggggatcgaa cccctgacct ttagaatgcc attctaacgc tctcccaact
       61 gagctacgcc cccgttcaag gtcaggtttt gcgcgaaagg gaccgtagca atcccttttt
      121 gttcttgaac ggtggcccta ttcgttacat acgggggaac gtggaacttg tcagttacat
      181 cctgatcgtc gtactcgtgg tgctcgccgg agcaggcgcc gtatacggat accagagcct
      241 ccgagggcct cgcaagctag aagaaatcgg gaatctgatc gcaggggaac gctatcgcga
      301 agctctagca gaatgccagc gaatcattca acaggatgat cgctccatga tcgctcattt
      361 tctggcaggt cagtgccacc aggccctcag tgagtatccc atggccgttg tagaatacaa
      421 gaattgtatt cgcattggca agtttgatga tcaggtcaaa gaaattccag tacgacgcgg
      481 cctcgcacga tccctgattg aatccggaaa cgcaaatgaa gcaaaaaatg aattcttaat
      541 cctcacaact cttgaaccca accagtttga aaatcatttt gaactgggca gattgtttca
      601 tcgcggcggc atctatccta aatccattaa attcttacag acagcaacgg cgcttaacgt
      661 taaaaatgca gaagcattca atgtgcttgg tcaatcgcac tatgcactca aacaatacca
      721 cgacgcccgg gccgcgctga tccgggcggt acaactaaaa ccagatctca aagcggctca
      781 ctactacctg ggactggctc ttcgctatct caacgatctg gactgggcgc tcaaagaatt
      841 cgaaatcgca gaaaaagacg atgggcttag agacaaagct cttctggcaa aaggaatggt
      901 ccttctggat caagggaatt tttcgaaggc aattactgaa ctcgaacgtg gattgaaatt
      961 cgccccacct ggatcggaaa ccatgattca gctctactat ctcattggag ttgcggccga
     1021 aaaggggcgc gacgttcaca aggcaatcgc aagctgggaa aagatagagc aaatcaaacc
     1081 aggctaccgt gatgtcagag acaaactcag acaatatcaa gaattcagaa cagacgattc
     1141 cgtgaaggat ttcttgatca tgaacacgac tcaatttgaa aatgcgtgca ggcgcatcat
     1201 tgagaagatg ggcttccagg tgcttcacct gatgctgaac ggagaaatga gtgttacggg
     1261 cgttggcaca gatgcagacg taaagagcag gaatcgcggg caaaaaactc tcttcttcat
     1321 atcacgcgat atggctgcgc tcccggaaaa aatagtcaga gagtttcacg aatctatgcg
     1381 cgaaaaaggc gcccttcgcg gcgtgatcat gactacagga gaagtgatgc cggccgctct
     1441 caactacgcg actacacgac ccatcgaaat ctatgactcc agcgcggttg ttcctttcat
     1501 ccgcactgcg atgaactaaa atgcatcacg aaaaagcggg cagtccaatt gtttcggctc
     1561 tgggaaacgg acgccttcag gtggttcgca atccggaagt ggattatatt ctggatctag
     1621 tactcgatga agccaggcgt ctcgtgcagg caagacaagg aaatttctat ctttttaact
     1681 caatcagcaa actggagcca tttcttcccg ctgccgcatc tgaacaggac atcgctctgt
     1741 gcgaagacgt catccaggca cgcacaagca gactgattcc cgagaatccg gccctgtgcg
     1801 tttacatgac cctgcaagaa ggagatctgg gcgtactctt gcttcgcgaa cccgcgtact
     1861 ttgatcagtt ctatgaagca gatctcgcgc ttgtgcagaa tctggccgct acatttgcaa
     1921 ttctcttgaa gagcgcattc attgatcaac gaacgccaga agtatttctg gggttcaaaa
     1981 gcagcctgct cttactcctt gaaaacgcgc acttaaatca caaaatcaaa gagtccgatc
     2041 accagctcaa gagtgttcta gaagtttcaa acttgatcaa ctcgtcgcgc gaactttcag
     2101 agatgatcca gacagttctt tacagtgcga agagagtgat tcgcgcagaa agcgcgagtc
     2161 tgttcctggt ggatgaaaaa acgggagaac tgtatttcga aatcattgcg ggcaacaaga
     2221 aagacctgaa aggctttcgc atccctcgcg gacagggcat cgcagggatc actgctgcca
     2281 cgaagaaatc tctcattgta aacgacgcgc aaaatgacgc gagagtgttt cgccaggtag
     2341 atcaggtatc cagagaaatc actcgcaata ttctggcggc acctcttcta atagaaggag
     2401 aagcaatcgg cgtcattgaa gtgatcaata cgatcgaccg tcccgctttc tctgagcacg
     2461 acctggaaat tttcgaaagt ttttctgact cggtggcgat cgccttacag cgaaggcgcc
     2521 tcctggatag cattcaaagg acaaacgtac agctggaaag aaggctccgc gagatcacaa
     2581 gtcttcacgc ggttgcacgt gcgcaggtgg aagaaagaac cattcaggat cttttcgcgc
     2641 gggcactcaa catcgtgcgg gacgatctca aagttggtcg tgcgtccatt ctcatggcaa
     2701 gcggatcgac tttgaaaatg gcggcctctg ccgggacata cgacgaaagc gatgcaaccc
     2761 ccggcagcct ggcccatcac gtattttcta ccaatcgacc tgtctttata gaggactttc
     2821 gcgagaatcc cgaatttgcg gctatggcca atccgcacag atacgattca agatcgtgca
     2881 tcattctgcc tctggcaagt ccaggccagc ctccttttgg cgtgctgtgt ctggcagaac
     2941 catcgagtgg gcaattcctc gaggaagatt acaaactcct tcttacgatc gcatctcaaa
     3001 tcgtcaaagg ttacgaaaac ctgggactca acgaacaaat catcgtgaag aaatctctgg
     3061 aaaaggaaat ggagattaca tctcgcatcc agagaaacat tctaccaata aaaaagccac
     3121 agcacctgca tctggatctg gcggccagat ccgtgatggc aaaaacaaca ggtggggatt
     3181 tcttcgacta ctatgtgcac actcccaatg gagatgtttc ctgcctcgtg gcggatgttt
     3241 cgggcaagag tcttcccgct gctctcttca tggcagtgtc cagttccatt cttcgcacga
     3301 tcatccgtca ggaaatcgat ccaacgcgag ttctggcgca ggcaaatgat ctgctgtttg
     3361 aagaatccca gtctggaatg ttcgtcactg tctttctatg cagatacgat ccgtccaccg
     3421 gtcggctgcg atatgcatcc gccggccaca atgaaatgat cctcatgcat gagaatggga
     3481 attatgaaat gttgtctggc aaaggttcac ccctgggcgt actgccggcg tatcgccaga
     3541 agtatctggg tggttcgacc caggtgcgcg atggggattt actcattctg tacacggacg
     3601 gcgtgatc
//
LOCUS       DAOQJL010000412         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16319_length_3603_cov_4.786922, whole genome shotgun sequence.
ACCESSION   DAOQJL010000412 DAOQJL010000000
VERSION     DAOQJL010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16319_length_3603_cov_4.786922"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..792)
                     /locus_tag="PLG78_15255"
     CDS             complement(178..792)
                     /locus_tag="PLG78_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE24530.1"
                     /translation="MSPEKAAATAAARFRLRAMFSMHGISYPPEGIALIGLKQEKLLE
                     VRAKTTSGWIKVKTYKVLKASGFPGPKLREGDKQVPEGIYRITTLNPESKYYLSLKLD
                     FPNEFDLMHADKEGRKEPGSDIYIHGKDISTGCLAMGDPAMDELYTMVMDAGMTNVTV
                     VIAPNDLRRRGPVTNMRAAPRWTQDLYKDIKTSLVKFSKTEKIR"
     gene            922..1587
                     /locus_tag="PLG78_15260"
     CDS             922..1587
                     /locus_tag="PLG78_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24531.1"
                     /translation="MRIIACLFMALTVISCRDGESPSSSDALLTLRSDDVEKARLSVL
                     TIAERRGQILESKLETGLETKFIARLQIPASAYYTTIKELSATGRLLSESTKVNLPTP
                     EKQSSPDPAAVLSPGPQNTSTAAVQTPAQTSVSVSKDKINIEVRISGPGSQDGLKIPA
                     FREAIIGVINAILGLLYVFIWISPLALLYGVYRLTRKKKKAPEPASLTTANQHGNPPS
                     TPA"
     gene            complement(1629..2864)
                     /locus_tag="PLG78_15265"
     CDS             complement(1629..2864)
                     /locus_tag="PLG78_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24532.1"
                     /translation="MRSIQLPVRVWLLLCFALSFCGNDSQPLAEWEGGSITRQELKYT
                     FQLLGKPPKLDSQEKVLEGLAVLKMSASESLRQGLADSPEFQKRSVFFEKTAFVGAFE
                     QFVAQETRKKKFKMMELQGAFLHDAATPKTREEEALDFVKRLNAASEIDVIQMIAERT
                     ELRRYGVVGGYMEPLCSNCPMNPYVFLINPARRAEGKFISIPDSNGFWIVRKVREEEF
                     ADDDLVEEYAHYYRKIVPIGRHFVRELPEGNERLALEVTIPADSDIAMTANQKAQAHL
                     VRESRLAYENKLREKKAEHHFEAGPNWNKSDQIANRAELLFKLDEKSFTLADFETEIP
                     ESARDKGLILHQVLFPYLLLKDDSLADPVRKSEYFKFLLEWNRNTALSELYIARLEPG
                     SEEKRMARMNALRAELPQK"
     gene            complement(2879..>3603)
                     /locus_tag="PLG78_15270"
     CDS             complement(2879..>3603)
                     /locus_tag="PLG78_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE24533.1"
                     /translation="NLGHQAVEFLTLEKMDQAWVDKYFEFEPTGAEAVRKAVREKKGQ
                     LFVLAHTGNWEMLNQPMTIHTGVHINLIIAPMSNPFGDKFLCNIRRSLGTTLIYPFDV
                     GPNVLKALARGEAAAFASDQNAGRRGLFIPFLGQPASTHTGAAVFSYLSGAEIFFITN
                     MRLPNGRFRTEILPMGSIDKKKVKDKDECIKEFTLRYVDILEKYIRKYPDQYFWLHNR
                     FKTKPLPEDEIIPRRDFDLKPY"
BASE COUNT          885 a          933 c          835 g          950 t
ORIGIN      
        1 catttggatg aaatgaaaac caccgctgag ttcacttttc agttcaatcc cctcggggag
       61 cgcgcccaga tgagtggaat agtccgaggg cggattgata aatatgacga tttgcccggg
      121 ttttaggccc agttttgact ggagtgcttt tttagaatat cctgccactg aattccctta
      181 tcggatcttt tccgttttac taaatttgac gagagaagtt ttaatatctt tgtacagatc
      241 ctgcgtccac cgaggagccg cgcgcatatt cgtaacgggc cctctccttc gcaagtcgtt
      301 tggggcgatc accacggtta cgtttgtcat tcctgcgtcc atcaccattg tatagagttc
      361 atccatcgca gggtccccca tcgcaaggca tccagtggaa atatcttttc catgaatgta
      421 gatgtcgcta ccaggctcct ttcgcccctc tttgtccgcg tgcatgagat cgaactcatt
      481 tggaaaatca agcttgagtg aaagataata tttactctca ggattgagag ttgtgattct
      541 gtagatgccc tcggggactt gcttgtcccc ttcgcgcaat ttcggtccag ggaaaccact
      601 tgccttcaac actttgtatg tctttacttt gatccagcct gacgtagtct tcgcacggac
      661 ctcaagaagc ttttcctgtt tgagcccaat cagtgcgatt ccctcaggag gataagaaat
      721 gccgtgcatg gaaaacatag cacgaagacg gaagcgcgct gcagctgtgg cggcagcttt
      781 ctcaggtgac atggtatcct ctgccctggc acagagcgga aaaaaaagaa agcaaatcac
      841 aaaaaatgaa cgaacccgct tgcaaccatt catgtccttt ggtaatattg tgacaaagat
      901 tccggaggtc aagtacacag aatgcgaatc atagcttgtt tgttcatggc cctgaccgtc
      961 atttcctgcc gcgatgggga gtctccctcc tcttccgatg ccctgctcac tctacgctcg
     1021 gatgatgtgg agaaagcacg cctgagtgtt ttgaccattg cggaaagacg cggccaaatc
     1081 ctggaaagca aactggagac cggactggag acaaagttca ttgcgcggct tcaaattcca
     1141 gcttctgcgt actacacaac catcaaagaa ctcagcgcca caggacgact cctgtctgaa
     1201 tctacgaaag taaacctgcc cacccctgaa aaacaatctt cgcctgaccc tgcggctgtt
     1261 ctttctcccg ggccgcaaaa tacgtcaaca gccgctgtgc aaacgcctgc gcagacaagc
     1321 gtttccgttt ccaaagacaa gatcaacatt gaagtcagaa tcagcgggcc tggaagtcag
     1381 gatggtttaa agattcctgc atttcgcgaa gcaatcatcg gagtcatcaa cgcaatcctc
     1441 ggacttctct atgtgttcat ctggatttct cctctggcac ttctctatgg cgtctatcgt
     1501 ctgaccagaa agaagaagaa agcaccggag ccggcgtctc ttacgactgc aaaccagcac
     1561 ggaaatcccc cgagcactcc tgcttagagt acgccgtcat ggagtctgtc tttgtgaatg
     1621 cgtaagctct atttctgcgg aagttcagcc cgcagcgcat tcattcttgc cattctcttt
     1681 tcttccgagc caggttcgag tctggcaatg tagagttcag aaagagcggt gtttctgttc
     1741 cactccagaa gaaatttgaa atactccgac tttcgcacag ggtcagccag agagtcatct
     1801 ttgagaagaa gatagggaaa aagaacctga tgcagaatca ggcctttgtc tctcgcactc
     1861 tcaggaatct cggtctcaaa atctgcgaga gtgaatgatt tctcgtccag cttgaagagc
     1921 aactctgcgc gattcgcaat ctgatctgat ttattccagt ttggaccggc ctcaaaatgg
     1981 tgctccgcct ttttttcgcg aagcttgttt tcataagcaa gtctggattc gcgaaccagg
     2041 tgcgcctgcg ctttctggtt agctgtcatt gcgatgtcac tgtctgctgg aatagttact
     2101 tccagggcca gcctttcatt tccttccggc aattcgcgaa cgaagtgtct gccaatcggc
     2161 acaatctttc gatagtagtg cgcatattct tccaccagat catcatccgc aaactcttcc
     2221 tcgcgaacct ttcgcacgat ccagaatccg ttgctatccg gaatggaaat aaacttccct
     2281 tccgctcttc gcgccggatt tattagaaat acatacggat tcatcgggca gttggaacaa
     2341 agcggttcca tgtagccgcc aactacgcca tatcgccgca attctgtcct ttcggcgatc
     2401 atttgaatca catctatttc actggccgca ttgagccttt tgacaaaatc gagagcttct
     2461 tcttcgcgcg tctttggagt ggcggcatcg tgcaagaaag caccttgcaa ttccatcatt
     2521 ttgaatttct tctttcgcgt ttcttgagca acaaactgct caaaggcgcc aacgaacgca
     2581 gtcttctcaa agaatacaga ccttttctga aactcaggcg aatccgcaag gccctgccga
     2641 agagactcgg acgcggacat cttgagcact gccagcccct cgagaacttt ttcctgcgaa
     2701 tctagttttg gcggtttccc cagaagctgg aatgtgtatt tcaactcctg ccgtgtgata
     2761 ctaccgcctt cccactctgc aaggggctgc gaatcattgc cgcaaaacga aagagcaaaa
     2821 caaagaagga gccaaacgcg taccggcagc tggatagaac gcatatgctt cttgatcctc
     2881 agtagggttt caaatcaaag tcgcggcgtg gaatgatttc gtcttcgggc aatggttttg
     2941 ttttgaaacg attgtgaagc cagaaatatt gatccggata ttttctgata tatttctcta
     3001 agatatcaac gtaacgcaga gtaaactctt tgatgcactc gtctttgtcc ttcactttct
     3061 ttttgtcgat gcttcccatg gggaggatct ctgtgcgaaa tcgtccattg ggcaaacgca
     3121 tgttcgtaat gaaaaagatt tcagcaccgc ttaaatatga aaacacagcg gccccggtgt
     3181 gcgtggaagc gggctggcca agaaacggaa tgaaaagtcc gcgacgccct gcattttgat
     3241 ccgacgcgaa agcagcagcc tctccacgag ccaatgcctt gaggacattc gggccaacat
     3301 caaatggata aatgagagta gtgccgaggc ttcgcctgat gttgcataga aacttgtccc
     3361 cgaacggatt ggacatgggt gcaatgatca agttgatatg aacgccggtg tgaatggtca
     3421 tgggctgatt gagcatttcc cagtttccag tgtgcgcgag tacaaagagc tggcctttct
     3481 tttcgcgcac tgcttttcgc actgcttccg cgcccgtggg ctcgaattca aagtacttgt
     3541 ccacccaggc ctgatccatt ttttccagtg ttaaaaattc aacagcctga tgacccagat
     3601 tct
//
LOCUS       DAOQJL010000413         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16405_length_3592_cov_4.499010, whole genome shotgun sequence.
ACCESSION   DAOQJL010000413 DAOQJL010000000
VERSION     DAOQJL010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16405_length_3592_cov_4.499010"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLG78_15275"
     CDS             <1..344
                     /locus_tag="PLG78_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24534.1"
                     /translation="ETMEEVGIFLEDRHYVAPVQEIQASARGSRMELIVFPFVFYWDG
                     DSELIHAADEVAATVWVPVDLVLDQTRHTTFMNQYEDKTVQMNCIEYEGYRIWGMTYR
                     MIQNLISAVNA"
     gene            345..734
                     /locus_tag="PLG78_15280"
     CDS             345..734
                     /locus_tag="PLG78_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE24535.1"
                     /translation="MQIHHIAITAREVPLVASFYETVLGLRRIREQSDSSGLRSVWLD
                     LAGAILMIERADRTAAPVGSFHERPPGYYLLAFRIAKEERESYIAKFAEKRVPVVYST
                     DFTIYILDPERNRIGLSFYEMDLDGAR"
     gene            complement(754..1659)
                     /locus_tag="PLG78_15285"
     CDS             complement(754..1659)
                     /locus_tag="PLG78_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE24536.1"
                     /translation="MSEIVSVINYKGGVGKTTLTLQIGVGLVTLFQKRILLVDLDPQC
                     SLSLSTVDEHYWADRVEKQGSVREMIQSFYESEKPECNPDWIIENALDRAWDSMPGKL
                     EGLDLLPGHLDLPDYEMKLVAKKPGHMNAEEYQLKRYMILKDALAKVRKKYDMILCDC
                     PPNIYMVARNAIIASDYFLVPTIPDFISCYGVPFILEHIKAMQEEVRERGGHTEAKFL
                     GIVRNRVRQAGSHLVKEHEEHSVKLRERYEGALFDSMIIDRIGVAELLGSRENLYTAK
                     SERSNEIKKDFTHLVREVLRRMGKD"
     gene            1768..3480
                     /gene="sulP"
                     /locus_tag="PLG78_15290"
     CDS             1768..3480
                     /gene="sulP"
                     /locus_tag="PLG78_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNE24537.1"
                     /translation="MLKPKLFTLLREMTPAQLLSDLTAGVIVGVVALPLAIAFAIASG
                     VSPERGLYTAIIGGFIISFLGGSRVQIGGPTGGFVVIVYAIVEHYGYPGLAAATLMAG
                     VILIIMGLARFGSAIKFIPHPVIVGFTSGIAVIIFSAQIRDVLGLSVEKVPADFIEKW
                     VEYVRHISSINVHAAAISGGCLLVLILWPLLTKRIPASLVALVGSTAIVYFLNLPVET
                     IGSRFGSIPSTLPAPSLPEISFQNVRNLIQPAFTIAMLGGIEALLSAVVADGMIGSKH
                     RSNMELVANGVANVLSPLFGGIPATGAIARTATNIKNGARTPVAGIIHAITLLLIMLL
                     LAPLARLIPMACLGAILIVVAYNMSEWRTFVALLKTPKSDVIVLLITFGLTVIVDLTV
                     AIEVGLVLAAFLFMNRMAHVTNIAQFEKEFEDSDDLDDPLSIHKRQVPDDVRVFEING
                     PFFFGAAYKFEEALQVMDRYPRVIIIRMRNVPAIDSTGMHVLKQVHRQAQSRGTQMVL
                     SGVHTQPLFAMEKAGMLTLFGEDNIFGNIDDALNRAREVLGLPRVERPGEFVPTVARE
                     GRPS"
BASE COUNT          802 a          872 c          936 g          982 t
ORIGIN      
        1 gcgaaaccat ggaagaagtg ggcatttttc tggaagatcg tcactatgtg gctccagtgc
       61 aggagatcca ggcatctgcg cgagggagca gaatggagct catcgtattt ccatttgtct
      121 tttactggga tggcgattcg gaattgattc acgccgcaga tgaggtggca gccacagtct
      181 gggttcccgt ggatttagta ctggatcaaa cacggcacac tacttttatg aatcagtatg
      241 aagacaagac agttcagatg aattgtattg aatacgaagg atacagaatc tggggaatga
      301 cgtatcgcat gattcaaaat ctgatttcgg cggtgaacgc atagatgcag attcatcata
      361 tcgcgatcac tgcgcgcgaa gtacccctgg ttgcttcttt ttatgaaacg gtgttgggct
      421 tgcgtcgaat tcgtgagcaa tcagattcat ctggcctacg atccgtctgg ttagatcttg
      481 cgggcgcgat tctcatgatc gaacgcgcgg atagaacggc tgcacctgtt ggaagctttc
      541 acgagcgtcc gccgggctac tatctccttg catttcgcat tgcaaaagaa gaaagggaat
      601 catacatcgc gaagttcgca gaaaagagag tgcctgttgt ttactcgaca gactttacaa
      661 tttacatcct ggatcccgag aggaaccgga ttggtctttc tttttatgaa atggatttgg
      721 acggggctcg ctgagtcgag aggcctggtt ttgttaatct tttcccatgc gacgaagaac
      781 ttcgcgtacc aggtgcgtga aatccttctt gatttcgttg gacctttctg acttcgcagt
      841 atagagattc tcgcggctgc caagaagttc cgccacacca atgcgatcaa tgatcatgga
      901 atcgaagagg gctccttcat atctttcgcg gagtttcaca gaatgctctt catgctcttt
      961 taccagatga gagcctgcct gccgcacccg atttctcaca attcccagga atttcgcctc
     1021 tgtatggccg cctctctctc tcacttcttc ctgcattgcc ttgatgtgct ctagaatgaa
     1081 aggaacaccg tagcatgaga taaaatccgg aattgtagga acgaggaagt agtcactggc
     1141 gatgattgca tttcgcgcaa ccatgtagat gttgggcgga cagtcgcaca ggatcatgtc
     1201 atattttttt ctgacttttg ccagcgcgtc tttgagaatc atgtagcgtt tgagctggta
     1261 ttcttccgcg ttcatgtgtc ctggcttttt tgccacgagt ttcatttcat agtctggcaa
     1321 atcgaggtga cccggaagta gatccagccc ctccagtttt ccgggcatgc tatcccaggc
     1381 gcggtccagt gcgttttcga tgatccaatc cgggttgcat tctggctttt ctgattcgta
     1441 gaatgactgg atcatttcgc gtacggagcc ctgtttctcc acgcgatctg cccagtagtg
     1501 ttcgtccacg gtggagagag acaggctgca ttgtggatct agatccacga gcaaaatgcg
     1561 cttctggaag agcgtgacca gccccactcc aatctggagc gtcagcgtcg tcttaccaac
     1621 tccgcccttg taattgatta cggaaactat ctcggacatc tgatggctat attatcggcc
     1681 gttctgccgg aaaaagtctg ccttccgcct gtgcgacgcg ggatttctgc ttgaaatccg
     1741 tttttgcggc ggtataatcc agggcgaatg ctcaagccaa agctctttac actccttcgc
     1801 gagatgacac cggcccagtt actgagcgat ctaacggcag gcgtgattgt cggagtagtg
     1861 gctcttcctc tcgccatcgc tttcgccatt gcgtctggag tgtcgccaga aagaggcctc
     1921 tatactgcga tcataggagg gttcatcatt tccttcctgg gtggaagccg ggtacagatc
     1981 ggcggtccca ccggtggctt tgtagtgatc gtatacgcga tcgtggaaca ctacggatac
     2041 ccgggccttg cggccgccac tctgatggcc ggggtgattc tgatcatcat gggcctcgcg
     2101 cgctttggct ccgccatcaa gttcattcct catccagtga tcgtagggtt tacaagcgga
     2161 atcgcggtga tcattttttc cgcacagatt cgcgacgtgc tgggcttgtc tgtggaaaaa
     2221 gtgcctgcgg attttatcga aaaatgggtc gagtacgtgc gtcacatcag ttccatcaat
     2281 gttcacgccg ccgccatttc aggtggatgt cttcttgttc tgattctttg gccgcttttg
     2341 acaaagcgca ttccggcatc tctggtcgca ctcgttgggt ctacagcaat tgtatatttt
     2401 ctaaaccttc cggtggaaac gattggaagc agattcggat ctatcccatc cacgttgccc
     2461 gctccctctc tgccagagat ttcttttcag aacgtgcgca acttgattca gcctgcattc
     2521 accattgcga tgctgggagg gattgaagct ttgctgtctg cagtcgttgc ggatggaatg
     2581 atcggaagca aacacagatc caacatggaa ctggtagcaa acggagtggc caatgttctt
     2641 tcgcctctgt ttggcggaat cccggcaaca ggagccatcg cgcgcaccgc gacgaacatc
     2701 aagaatggag cgcgcactcc cgttgctgga atcattcacg ccattacact tcttctgatc
     2761 atgcttcttc tcgcacctct tgcgcgattg ataccaatgg cgtgcctcgg agcgatcctg
     2821 attgtagtcg cttacaacat gagcgaatgg agaacctttg tagcactgtt gaaaactcca
     2881 aagagcgatg tcattgttct tctcattaca tttggattga ccgtgatcgt ggatctgact
     2941 gtggccattg aagtgggtct ggttctcgcg gcgtttctgt tcatgaatcg catggcgcat
     3001 gtaacaaaca ttgcacagtt tgaaaaggaa tttgaagact cagatgatct cgatgatcct
     3061 ctttcgattc acaaaaggca ggtaccggac gacgtacgtg tttttgaaat caatggccct
     3121 ttcttttttg gagccgctta taagtttgaa gaagcgctac aagtgatgga tcgctatccg
     3181 cgggtgatca tcattcgcat gaggaacgtg ccggccattg actctacggg aatgcatgta
     3241 ctcaagcagg tgcataggca ggcgcaaagc agaggaacgc agatggtttt gtctggtgtc
     3301 catactcagc cgttattcgc catggaaaag gcgggaatgt tgacgttatt cggcgaagat
     3361 aatattttcg gaaatatcga tgatgcgttg aaccgggcca gagaggttct gggacttccc
     3421 agggtagaac gaccaggaga atttgttcct acggtggccc gcgaaggccg gccctcgtag
     3481 ttcagttctg gttttgattt tcgctttcgc gtcgtttctc aaaaactcct gaaagaattt
     3541 tcgagcctgc agttaagaat gcggccgcta tcctggccgc aaccaggaga aa
//
LOCUS       DAOQJL010000414         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16518_length_3577_cov_3.020727, whole genome shotgun sequence.
ACCESSION   DAOQJL010000414 DAOQJL010000000
VERSION     DAOQJL010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16518_length_3577_cov_3.020727"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLG78_15295"
     CDS             <1..594
                     /locus_tag="PLG78_15295"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24538.1"
                     /translation="WNSHRKLKLESLSRARIKESSMFCVECGTKFPEGAQFCPGCGTA
                     RTKSANAMAPSGNADIHADYVEYMALPAGPAREQFMRDKAGSIPRYIAAAEADDAEAC
                     FLAGRAYDVGVSGYEKNDVEAAKWYRKAAGQGNAVCQFNLGNMYADGRGMAQDEAEAV
                     KWYRKSAEQGHAKAQANLAFAQFNLGLQEIWADLEKK"
     gene            complement(633..1238)
                     /locus_tag="PLG78_15300"
     CDS             complement(633..1238)
                     /locus_tag="PLG78_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24539.1"
                     /translation="MTQNEGGVQNGGSLRYSLHRLAYQGKPVLTFLFKNRTDLLQHFD
                     RLAEGRLSAVGETLEGASINKWDYYRFYVRNGIVKTEAEQSVFESVRPFMRQKFSTIA
                     LMEGFHEKYLIHEQTHALYELDREFRRFVKAYVDSRPLMKGRLVQNVKKYYRVDRISD
                     SELYSEYAATLVETEYYTSDDEICRWYERFVEETTGVRQTE"
     gene            complement(1225..2004)
                     /locus_tag="PLG78_15305"
     CDS             complement(1225..2004)
                     /locus_tag="PLG78_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24540.1"
                     /translation="MTQYFRRVRWEEKRIENAQLFILRFPNPSLMVLTLLRISEFKEC
                     SVHAGTIFSNTRYRTWFRSGHNGYDYDDFVVGMNLPDANFTNFFEAFVQARGRNRINR
                     FERTFLAVLEERGLIQLQGNRASVRQKFYVVVLNEFLIGNAAEDEEGQLAIEYATLRH
                     ELSHAAYYLSTEYREAVQKAWDGLDPQTRNNVMLFLAEKHYTRDVIVDEFGAYLMEPY
                     DSHFPVDKEQYAEAVHRMTQLYSECVANLLPEAGRIPYDAK"
     gene            2371..2817
                     /locus_tag="PLG78_15310"
     CDS             2371..2817
                     /locus_tag="PLG78_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24541.1"
                     /translation="MKKAAREDWTEAWGSREVVMLELTVAVASLFLEDGIDRYPFGTV
                     KMFRKGKKEYVLKSYPSTSVGNLHHLLLEIATEVFARQPKARQAALEVAYEYLESLTV
                     DELKEIRGDEDSINAAAEVCTRLQKKMSDLHGISAAAKKQTAFHDP"
     gene            3163..>3577
                     /locus_tag="PLG78_15315"
     CDS             3163..>3577
                     /locus_tag="PLG78_15315"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24542.1"
                     /translation="MNASHRDYCAYMALPRGPDRERFIRERSDALPEYLEAATAGDAE
                     ACFLLGRAHDVGSAGGNDSPNEAVKWYRKAAELGNVFAQYNLGVIYEGGRGIEKDEVE
                     AARWYRKAADEGLADAQRALGNLYYSGRGLEKDEVE"
BASE COUNT          844 a          899 c          953 g          881 t
ORIGIN      
        1 tggaattccc atcggaagtt gaaactggaa tctttgtccc gagctaggat caaggagtca
       61 tcgatgtttt gtgtagagtg cggtacaaaa ttccccgagg gtgcacaatt ctgtcccggt
      121 tgtggaactg cgcgcacgaa gtccgctaat gcgatggctc catccggcaa cgctgatatc
      181 catgctgact acgttgagta catggctcta ccagccgggc cggcgagaga gcaattcatg
      241 cgcgacaagg ctggcagcat tccgcggtat attgctgccg cagaggcgga cgatgcggag
      301 gcttgcttcc ttgcgggccg cgcctacgac gtaggagtct cgggttatga gaagaacgac
      361 gtggaggcgg caaagtggta ccgtaaggcc gcggggcagg ggaatgctgt ttgccagttt
      421 aatctcggga acatgtatgc ggacggccgc ggcatggcac aggatgaggc cgaagcggta
      481 aagtggtacc ggaagtcagc ggagcagggg cacgccaagg cccaggccaa cctcgccttt
      541 gcccagttca acctcggatt gcaagagatt tgggctgatt tggaaaagaa gtaacatttc
      601 gcctaacggc aaatgtccag gtcatcgaac ggctactcgg tctgccgaac ccctgtcgtt
      661 tcctctacaa acctttcata ccaacggcaa atttcatcat ccgatgtgta gtattcggtc
      721 tcgaccaggg tagccgcata ttcactgtag agttcgctgt cgctgattcg atcaactcga
      781 tagtactttt ttacgttctg cacaagcctt cccttcatca agggccggct gtcgacatat
      841 gctttaacaa atctcctgaa ttctcggtcc agctcataca aagcgtgtgt ctgctcatgg
      901 atgagatact tctcgtgaaa tccttccatc agagcgattg ttgagaactt ctggcgcatg
      961 aatggtctga cgctttcaaa aacactttgt tccgcttccg tcttcacgat tccgtttcgc
     1021 acataaaacc tgtaatagtc ccacttgttg atagatgcgc cttctagtgt ttccccgact
     1081 gcgctcaatc ttccctcagc gagcctgtca aaatgctgaa gcagatctgt tctattcttg
     1141 aaaagaaaag ttagaactgg tttgccctgg taggcaagac ggtgcagcga ataccgcagc
     1201 gatccgccat tttgtacgcc cccttcattt tgcgtcatac gggatccgtc cggcttccgg
     1261 cagcagattt gcgacgcact ccgagtaaag ttgcgtcatt ctgtggacag cttctgcgta
     1321 ttgctctttg tcgaccggga aatgactgtc gtagggttcc attagatagg ctccgaattc
     1381 gtcgacaatg acatccctcg tgtagtgttt ttctgccaga aagagcatta cattattccg
     1441 agtttgggga tccagtccgt cccatgcctt ttggacggct tcccggtatt ctgtggaaag
     1501 atagtaggcc gcgtgcgaaa gctcatgtcg gagcgtcgca tactcgattg cgagctgccc
     1561 ttcttcgtct tctgcggcgt taccgatgag aaactcattt agaaccacaa cgtagaactt
     1621 ctgtcgcacg gatgcccggt tcccctgcag ctgaatcagt cccctttcct cgagtacggc
     1681 aaggaatgta cgctcgaacc tgttgatgcg attgcgtcca cgggcttgta caaaagcctc
     1741 aaaaaaattc gtgaaattcg cgtcaggcag gttcatgcct acaacaaagt cgtcatagtc
     1801 gtacccattg tggccgcttc gaaaccaggt ccgatagcgt gtgttcgaaa atattgttcc
     1861 cgcatgcaca ctgcattcct tgaattcgct gatccgcagg agcgtcagga ccatcagact
     1921 cggattgggg aaccgaagga tgaacagctg agcattctca atccttttct cttcccatcg
     1981 aacgcgtctg aaatactgag tcatactccc ccgcagcaca caacggctcc gatgttctta
     2041 tcatttcgcg ttctgtgatt ttttgcaaga agaatgagaa acccggcatt gcgctggatt
     2101 gcggcgcgcg gaattgttcg cgaattgggt tgccacgcct gagtgagagc ttatggtgtt
     2161 cgcgatttcg accccgaaca gatgcccctc actgggacta ccgctatgaa ccacaagaat
     2221 cgcttcaaaa tcaattgtgt cgagtgcggc gctctaatgg acgacgaaac gtacctggat
     2281 gcggacggag ggtggttttt ttttcggacg aaattctcct gcaatccgtg caagctttcc
     2341 tatttgacca ggcggacgaa cttcaataca gtgaagaagg ctgcgcgtga agattggaca
     2401 gaagcatggg gcagtcgaga ggtcgtcatg cttgaactga ccgtcgcggt ggcgtccctg
     2461 tttttggaag acggaatcga tcgatatcca tttggcaccg tgaagatgtt ccggaaggga
     2521 aaaaaggaat acgttctcaa atcgtatcct tccacgagcg tgggcaatct gcaccatctc
     2581 ctgcttgaaa tcgcaacgga ggtttttgca cgtcaaccga aggcgaggca ggcggcgctg
     2641 gaagttgctt acgaatatct ggaatcgttg acggttgatg aactcaaaga aatccgcggc
     2701 gatgaagatt ctataaatgc ggcggccgaa gtctgcaccc gactccagaa gaaaatgtca
     2761 gatttgcatg gcatttcggc cgcggcgaaa aaacagacag cctttcacga cccgtgaaga
     2821 ttcacgcgat tccgagccgc ggcaaacaaa accggcctct acttcataac agaattgagt
     2881 ttcccattct gtatgtgcac gcgaaatgtc ttgtggcact tctgacaggt tactccttca
     2941 gttccatttg atgtcgttgt cttgtaagtc cctggttgtc cgcaatttgg gcaattgcac
     3001 tgaacagttg gttgagccat agtttctccc ctatcctcga tgttcttcga aacggattgc
     3061 ccgccgaatc ctgctcgtca atcaagaaat agaactgcct ctttcttgtt gacaagaccc
     3121 gagaatatct ttgtctccag gcattcagcc gtggttcgtg tcatgaatgc ttctcataga
     3181 gactattgtg cctacatggc cctgcccaga gggcctgacc gcgaacgatt cattagagaa
     3241 agatcagatg ctctgcccga gtatctagaa gcggctacag cgggcgatgc agaggcctgc
     3301 tttctattgg gaagagcaca tgatgttgga tccgccggcg gcaacgacag cccgaatgaa
     3361 gcagtcaaat ggtatcgcaa agccgcagag ctagggaatg tctttgcaca gtataacctg
     3421 ggtgtgattt atgaaggcgg ccgagggatt gaaaaagatg aagtcgaggc tgcgaggtgg
     3481 tatagaaaag cggcggatga agggcttgcc gatgcacaga gggcactggg caacttgtac
     3541 tactcggggc gcggcctcga aaaagatgag gtggagg
//
LOCUS       DAOQJL010000415         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16526_length_3576_cov_3.859983, whole genome shotgun sequence.
ACCESSION   DAOQJL010000415 DAOQJL010000000
VERSION     DAOQJL010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_3576_cov_3.859983"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="obgE"
                     /locus_tag="PLG78_15320"
     CDS             <1..1009
                     /gene="obgE"
                     /locus_tag="PLG78_15320"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNE24543.1"
                     /translation="DGGDGGRGGDVILQARSAYQSLGHFKNRKVFAADAGEPGDANQC
                     TGSNGNDLIVFVPPGTEVIDADTDEIVCDLIGIDSRITVVRGGKGGLGNVHFANSVNQ
                     APTYAQSGLPGEERNLILRLKLMADVGLVGLPNAGKSTLIAALSASKSRIADYPFTTL
                     IPHLGVLENANYRRLLLADIPGIIEGAHHGAGLGLSFLRHIERVRAIVYVIDVQSMDP
                     AAELSMLQAELASYSESLLARPFIVVLNKMDTIDYDEELAKQTADDVVEKLHNAKLGA
                     HDILSVSAKEKWHLDRLKQTLFEHFPGPTFAESLLVHGEEGEHILEEIAQDNREQGES
                     L"
     gene            1006..2088
                     /gene="proB"
                     /locus_tag="PLG78_15325"
     CDS             1006..2088
                     /gene="proB"
                     /locus_tag="PLG78_15325"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNE24544.1"
                     /translation="MTRADYNARLSRAKRVVLKVGTSLLTDESGIQTEMLEKLRREIL
                     FLRGRGCEVALVTSGAVGTARAILRDRSNTGELARKQALAAIGQGRLIAQYEKVFGKD
                     GILVAQLLITARDMRDRRAYLNIGNTLEELISMGVVPIINENDTVSTDELKFGDNDLL
                     SAACSSLFGADLLIILTAAPGFLIQDQRVSHLETISASELSHALGPAGPGSGGMRTKL
                     RAGQLCLISGQMLAILPGLHDSPVQSLFAGEDIGTLIAGSHKKRISARKKWLLFARPE
                     GEIVIDAGAQNALIEKGSSLLRAGIQELRGNFLAGDPVDVLSSSGEVLGRGIVNYSKR
                     DLDHPEDQRRLHGPREVIHRDNLILE"
     gene            complement(2125..3159)
                     /locus_tag="PLG78_15330"
     CDS             complement(2125..3159)
                     /locus_tag="PLG78_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNE24545.1"
                     /translation="MSAPLSTEKLTPKDFASSQEVKWCPGCGDYSILKQVQTVFPELE
                     VEREKFVIVSGIGCSSRFPYYMETYGMHSIHGRAFAVASGLKATNPDLHVWVVTGDGD
                     ALSIGGNHFIHLMRRNFDIKVMMFNNQIYGLTKGQYSPTSEKGKQTKSTPYGSIDEPF
                     NPAELALGAKASFVARTLDRDPKHMQAMIRRSHEHKGTSFLEIYQNCPVFNDAAFFPF
                     TDRETRKEESLFVEDGMPLIFGQNDEKGIKLDGLTPVVVDLNSSGVSRDDLWIHKEGD
                     KTKAGILARFFEQPGHHGLPRPFGVFYSEVRACYEDDLDQQIEHSRNTKGKIPLSKIL
                     SGDRTWVIAD"
     gene            complement(3156..>3576)
                     /locus_tag="PLG78_15335"
     CDS             complement(3156..>3576)
                     /locus_tag="PLG78_15335"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNE24546.1"
                     /translation="GNVSYDPANHERMVKIRAEKVEKIADSIPPAKVDSGREGAKVCV
                     LGWGSTYGSIKGAVTELIAEGHDVAHIHLRHLNPFPKNLGDILRGFGKVLIPEINNGQ
                     LVQLIRAKYLIPAVPLNKIMGLPFATTEIKSTIKEML"
BASE COUNT          888 a          878 c          918 g          892 t
ORIGIN      
        1 agacggcgga gatggaggca gaggcgggga cgtcatcctg caagctcgat ctgcgtacca
       61 gagtctcgga catttcaaaa atagaaaagt cttcgcagca gatgctggag aaccaggcga
      121 tgcaaatcaa tgcactggat caaacggcaa tgacctcatc gtattcgttc ctcctggtac
      181 tgaggtaatt gatgcagata cggacgagat tgtatgtgat ctaattggga ttgattctcg
      241 cataactgtg gttcggggcg gcaaaggcgg actcggaaat gttcactttg caaattcagt
      301 caatcaggct cccacgtatg cgcagtcagg acttccaggg gaagaaagaa atctaattct
      361 ccgcctgaag ctcatggccg atgtcgggct cgttggtttg cccaatgcgg ggaagtccac
      421 tttgattgcc gcactttccg caagcaaatc gcgcatcgca gattatccat tcactacatt
      481 gattcctcat cttggcgtgc tcgagaatgc caactatcgt cgacttttgc tcgcagatat
      541 tcctggaatc atagagggcg cccatcatgg ggccggactt ggtctttcat ttctccgtca
      601 tattgaacgt gtgcgtgcga tcgtctatgt gatcgatgtt cagtccatgg atcctgcagc
      661 agaactatcg atgcttcagg cagagctcgc aagctattca gaatctcttc ttgcgcgacc
      721 tttcatcgtt gtactcaaca aaatggatac gatcgactac gacgaagaac tcgcgaaaca
      781 aaccgcagac gacgtggttg aaaaactgca caatgcgaag ctaggagctc atgatatact
      841 atctgtctct gccaaagaaa aatggcatct ggatcgtcta aagcaaacgt tattcgaaca
      901 ttttcctggt cctacattcg cggaaagcct cctggttcat ggagaagaag gagagcatat
      961 tcttgaagaa attgctcagg acaaccgcga acagggagaa tccttgtgac tcgcgcggat
     1021 tacaacgcac gcctgagccg ggcaaaacgt gtcgtcttaa aagtcggcac aagccttctc
     1081 acagatgaaa gtggcatcca gacagaaatg cttgaaaagt tgcgacgcga gattctattt
     1141 cttcgcggtc gcggctgcga agtcgctctt gtaaccagtg gtgctgttgg aactgcgcgt
     1201 gcgattcttc gcgatcggag caatacaggc gagctggcgc gaaagcaggc tctcgctgcg
     1261 attggccagg gaagactcat tgcgcagtac gagaaagtct ttggcaaaga tggaatcctg
     1321 gtagcgcagc ttctcatcac tgcgcgcgat atgagagaca gacgagctta tctgaacatt
     1381 ggaaacacac ttgaggaact catatcgatg ggagtcgttc ccatcatcaa tgaaaatgat
     1441 actgtttcta cagatgaatt gaagttcggt gacaacgatc tgctgagtgc tgcctgtagc
     1501 tccctgtttg gtgccgatct gctcatcatc ctcaccgccg cacctggatt tctcattcag
     1561 gaccagagag tatctcatct cgaaacaatt tctgcatctg agctttcgca cgcactgggt
     1621 cctgccggtc caggaagcgg aggaatgaga accaaacttc gggccggcca gctttgtctg
     1681 atttccgggc agatgcttgc cattcttcca ggcttgcatg attcgcctgt tcaatccctg
     1741 ttcgcgggtg aagacattgg cactctgatt gccggatcgc acaagaaacg aatcagtgct
     1801 agaaagaagt ggctcctgtt tgctcgtccg gaaggtgaaa tcgtaatcga tgcaggcgca
     1861 caaaacgccc tgatagaaaa gggatcctct ctacttcgcg cagggatcca ggagctgcgc
     1921 ggaaattttc tcgcaggaga tccagtagac gttttgagca gcagtggaga agttctcggg
     1981 cgcggaattg taaactacag caaacgcgat ctggatcatc cagaggatca gcgacgcctc
     2041 cacggaccac gcgaagtgat ccacagagac aacctgatcc tcgaatgatt cagagccgga
     2101 cgaacgcagg gcgcaaatgc agacttagtc tgcgatgacc caggtgcgat cgccggatag
     2161 aatcttgctc agaggaattt ttccctttgt gttgcgagag tgctcaatct gctgatccag
     2221 atcatcttca tagcaggcgc gcacttcaga atagaaaact ccaaacggtc tgggaaggcc
     2281 atggtgtcct ggttgttcga agaatcgagc gaggattccg gcctttgtct tgtccccttc
     2341 tttgtggatc cagagatcat cgcggctgac tccacttgag ttcaaatcca caaccactgg
     2401 tgtcagccca tcgagcttga ttcccttctc atcgttctgg ccgaaaatca atggcattcc
     2461 atcttccacg aagagagatt cttctttgcg cgtttcgcgg tccgtaaagg gaaagaaagc
     2521 ggcatcattg aataccgggc aattctgata gatttcaaga aagcttgtgc ctttgtgttc
     2581 gtgcgatctg cgaatcatcg cttgcatatg cttgggatcg cggtccagtg tgcgcgctac
     2641 aaacgaagct ttggcaccca gcgccaattc tgccggattg aaaggttcgt ctatggatcc
     2701 gtaaggagta gactttgtct gttttccttt ttcagaagtt ggcgaatact gacccttggt
     2761 aagcccatag atctgattgt tgaacatcat gactttgatg tcaaagttac gacgcatcag
     2821 gtgaatgaag tgatttccgc caatagaaag agcatctccg tctccagtca ccacccaaac
     2881 atgcaaatca gggttggttg ccttgagtcc ggaagcaaca gcgaaggcac gtccgtgaat
     2941 gctatgcatt ccataagttt ccatgtaata cggaaagcga gaggaacagc caatcccaga
     3001 aacaatgaca aacttttccc gttccacttc cagttccggg aaaactgttt gcacttgttt
     3061 tagaatggaa tagtccccgc agccggggca ccattttact tcctgactgg aagcgaagtc
     3121 ctttggagtg agtttctccg tagagagggg agcgctcata gcatctcctt gatggtagat
     3181 ttgatttctg tcgtagcgaa ggggagcccc atgattttgt ttagaggcac ggccgggatc
     3241 agatactttg cacggatcaa ctggaccagc tgcccgttgt tgatttcagg aatgagtact
     3301 tttccaaaac cgcggagtat atcccccaga ttctttggaa atggattgag gtggcgaagg
     3361 tgaatgtgag ccacgtcatg accttctgcg atcagctctg tcacagcgcc cttgatggag
     3421 ccgtaggtag atccccatcc gagaacacac actttcgcgc cttcgcggcc tgaatccact
     3481 ttcgcaggtg gaatgctgtc cgcgatcttt tctacttttt cagcgcgaat tttcaccatt
     3541 ctttcgtggt ttgctggatc gtaagaaacg tttcca
//
LOCUS       DAOQJL010000416         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16566_length_3569_cov_4.075982, whole genome shotgun sequence.
ACCESSION   DAOQJL010000416 DAOQJL010000000
VERSION     DAOQJL010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16566_length_3569_cov_4.075982"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="PLG78_15340"
     CDS             <1..2048
                     /locus_tag="PLG78_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE24547.1"
                     /translation="FDEDFNGQVIAESHEQGMESYLGLHYPASDIPVQARELYRKNMI
                     RTIVDVSYEPVGIVSRDPAPLDMSYSYLRSVSPIHLEYLKNMGVAATLTVSIIVDNAL
                     WGLVSCHHRTPYLPHMKRVGLAEVFGNIFGGMIQMREESERDRVSGRMLSRLDTVMDI
                     VMSQDKNQDVMDLIDQRSFLFQSIFPSDGFLVRTDSQIVRRQFDLDDKLLTGLLDRLA
                     PLMRGSIFYTDHLAAVLPDLPAKTLRECAGVMALQLDTRPPSLWLWRRSEKTQTITWG
                     GDPNQKVILNQQGVISPRKSFEKYNQIVTGQSTRWNSAERDLPRYLTAQIYRLFEFFE
                     SSHQLENQKKQIQFMEEEKAKHYQELIEMLVGVIEQRDAYTAGHTRRVADYCVAIAQK
                     MGLDEASVSTLREAAILHDIGKVIIPDSILLKPGRLSFKEYELIKAHLSVGYQILSRI
                     DYYKPLAEIIKHHHEKFDGTGYPEGKKGDEIPLLSHIMIVADAFDAMTTNRIYQPRKT
                     TDDAVAELTTYRGRWYHPEVVDAAVAVAREMETSGTTPSQLPLTQMEKERFAYFFRDQ
                     LTGVYNGTYLWMVVNNLIPDQHCNFCLMIELRGMSEHNARFGWHEGNTVIRRFAEALV
                     KMVREEQLFRVFGDDFVVCFDDQPAREAFLRAWQPLMIESVTATCRVVERTAFLEIL"
     gene            complement(2049..3155)
                     /locus_tag="PLG78_15345"
     CDS             complement(2049..3155)
                     /locus_tag="PLG78_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24548.1"
                     /translation="MRKRLLLPLATCGFLTCVALPVKVAEVIPSAKNITIYSAGGSVV
                     SGTAAAPSSDILKIRLPATAQGIAIVQNDQPLKWFTTQMISEPGKDPNVVQKYLQVGI
                     PAARTGEVKFSYGLPEIKWAPRLTASILDAKNVNLRLEAAITMNATETFKNCSVTLVL
                     NNAVSLEKITGHTFNLSLTELAPGRNVAYNLESKNVSYSLLREWRTYGSKDVVHVLMQ
                     TRNPFSVDLSQTGFAVESHQISIESGSIDEPVPPGELFSLDAGIDDSITTFRDIKITE
                     TPSKKPLPFNHKLTYRITNNSDQTKVLRLVSDRVTGVDHRSIYHFTREPDATPEKTLV
                     WLVTLKPHSTETLQYDYDADLREVAGETGFESGM"
     gene            3269..>3569
                     /locus_tag="PLG78_15350"
     CDS             3269..>3569
                     /locus_tag="PLG78_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HNE24549.1"
                     /translation="MATNYVRFRFRSSVDWGLLGEEGRIAPLRAGDLSTRAFLDFLRR
                     KKRPAPRFTTTVREVAILSPISAPAQIICQGANYRQHMIESGLNPDQKRYNMFFTK"
BASE COUNT          833 a          860 c          947 g          929 t
ORIGIN      
        1 ggttcgatga agatttcaat ggtcaggtga tcgcagaatc gcacgagcag ggaatggaat
       61 cttatttggg cttgcactat cccgctagcg acattcctgt gcaggcgcgt gagctgtatc
      121 gaaagaacat gattcgcacg atcgtagatg tgagctatga gccagttggg attgtttcgc
      181 gcgatcctgc gccgctcgac atgagttata gttacctgcg aagcgtttcc cccattcatc
      241 tggagtacct gaagaacatg ggtgtggccg ccacactgac agtctccatc attgtcgaca
      301 atgcgctctg gggacttgtc agttgtcatc atcgcacacc gtatttgcct cacatgaagc
      361 gcgttggcct tgcggaagtt ttcggcaata tatttggcgg gatgattcag atgcgtgaag
      421 agagtgaacg cgatcgtgtg agcggtcgta tgctttcgcg tctggatact gtcatggaca
      481 ttgtcatgag ccaggataag aaccaggacg tgatggatct tattgatcaa agaagctttc
      541 tgtttcagtc catttttcca tcggatggat ttctggtgcg gaccgattcg cagatcgtca
      601 gacgccaatt cgatctagat gataagttgc tcaccggact actcgataga cttgccccgc
      661 tcatgcgggg atccatcttc tatacggatc atctggcggc ggtattgcct gatcttccag
      721 caaaaactct acgagagtgc gcgggggtta tggctcttca actcgacacc aggccaccaa
      781 gtctgtggct ctggcggagg agcgaaaaga cccagaccat cacatggggc ggggatccaa
      841 atcaaaaagt gattttaaat cagcagggtg tcatctcgcc gcgaaaatca ttcgagaaat
      901 acaatcagat cgtgaccggc caatccacgc gctggaattc ggccgaacgc gatcttcctc
      961 ggtaccttac tgcacagatc tatcgattgt ttgagttttt cgaatcaagc catcagctgg
     1021 agaatcaaaa gaagcagatc cagttcatgg aggaagagaa ggccaagcat tatcaggaat
     1081 tgatcgagat gctcgtcggt gtcattgagc agcgcgacgc gtacacggcc ggtcatactc
     1141 gtcgcgtcgc agattattgc gttgcgattg cgcaaaagat gggccttgat gaggccagtg
     1201 tttctactct gcgcgaagcg gctattcttc atgacatagg aaaggtgatc atccctgatt
     1261 ctattctcct caagcccggg cgactctcct tcaaggaata tgaactcatc aaagcacact
     1321 tgagcgtcgg gtatcagatt ttgtcgcgca ttgattatta caaaccgctg gcggaaatca
     1381 tcaaacacca tcacgagaaa ttcgacggga caggttatcc cgaaggtaag aaaggagacg
     1441 aaattcccct tctttcgcac atcatgatcg ttgcggacgc atttgacgcg atgaccacta
     1501 acagaatcta tcaaccgcgc aaaacaacag atgatgcagt ggcggaactt accacatatc
     1561 gaggcagatg gtatcatccg gaagttgtgg atgcagcggt tgcggttgct cgcgaaatgg
     1621 aaacaagcgg caccactcct tcgcaattgc ctctgacgca aatggagaaa gaacgattcg
     1681 cttacttctt tcgtgaccag ttgacgggcg tatacaacgg aacctacctc tggatggtgg
     1741 tgaataatct cattccagat caacattgca atttttgtct catgattgag cttcgcggaa
     1801 tgtcagagca caacgcgcga tttggctggc acgaaggaaa taccgttatt cggcgtttcg
     1861 cagaggctct tgtgaagatg gtgcgcgagg agcaactgtt tcgcgttttc ggggatgatt
     1921 ttgttgtttg ttttgacgat cagcccgcgc gagaagcgtt cctcagggcc tggcagcccc
     1981 tcatgattga atctgtcaca gccacctgtc gcgtggtgga gcgaactgca tttcttgaga
     2041 ttctttaatt acattccaga ctcaaaccca gtttcgcccg ccacttctct cagatccgcg
     2101 tcgtagtcat actgcaatgt ttccgtgcta tgaggcttga gagtgacaag ccaaacaaga
     2161 gttttctccg gggtggcatc cggttcgcgc gtgaagtggt agatggatct gtgatctaca
     2221 cctgtgaccc ggtctgacac aaggcgcagc acttttgttt ggtctgaatt gtttgtgatc
     2281 ctgtacgtga gcttgtgatt gaaaggcaga ggctttttgg atggagtctc cgttattttg
     2341 atatcgcgaa atgttgtgat ggaatcgtcg atcccggcat cgagggagaa gagctcaccg
     2401 ggtggaaccg gctcatcaat ggaaccggat tcgatgctga tctggtgcga ttctactgcg
     2461 aagccggtct gagaaaggtc aacagaaaat ggattcctgg tctgcatcag cacatgcact
     2521 acgtctttgc tcccataggt gcgccattca cgaagcagag agtagcttac gtttttactc
     2581 tcgagattgt aggctacatt tcgaccggga gccagttcag tcaggctcaa gttgaatgtg
     2641 tggcctgtga ttttctcaag gctcactgcg ttattgagta cgagtgtgac gctgcagttc
     2701 ttgaatgttt ctgtagcgtt catcgtgatg gcagcttcga gtcttagatt cacattcttc
     2761 gcgtccagga tagaggcggt tagcctgggg gcccatttga tttcgggcag tccatagctg
     2821 aatttcactt cccctgttcg ggcggctgga attccaacct gcaaatattt ttgaactacg
     2881 ttcggatctt tgccaggttc ggaaatcatc tgagtcgtaa accatttgag cggctgatca
     2941 ttttgaacaa tcgcgattcc ttgcgctgtg gccggtaaac gaatcttcaa gatgtccgaa
     3001 ctcggtgccg cggccgttcc cgaaaccacg cttccccccg cgctatagat ggtgatgttt
     3061 tttgcggatg gaatcacttc cgccactttt acggggaggg ccacgcacgt gaggaatccg
     3121 caggtggcta gaggcaatag aaggcgcttg cgcataacgt gatttcgcga tcacgcgaga
     3181 tccgagtcaa caaattctga ctgttttgat cgttcgcatt gtgatatatt gaattgactg
     3241 ctgtagtcca ggttcggcca tctcatggat ggctacgaac tatgtgcgct ttcgctttcg
     3301 ctcttcggtg gactgggggc ttctgggtga ggagggcagg atcgcgcccc ttcgcgcagg
     3361 tgacctgagc actcgcgcct ttcttgattt tctcagacgc aagaagagac ccgcgccacg
     3421 gtttacgact accgtgaggg aagtagctat tctttccccc atttctgctc ctgctcaaat
     3481 catctgccag ggagccaact acaggcagca tatgatcgag tccggactca atcccgatca
     3541 aaaaagatac aacatgtttt tcaccaagt
//
LOCUS       DAOQJL010000417         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16568_length_3569_cov_3.651679, whole genome shotgun sequence.
ACCESSION   DAOQJL010000417 DAOQJL010000000
VERSION     DAOQJL010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16568_length_3569_cov_3.651679"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /gene="mgtA"
                     /locus_tag="PLG78_15355"
     CDS             <1..2066
                     /gene="mgtA"
                     /locus_tag="PLG78_15355"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNE24550.1"
                     /translation="NQAALTGESMPVEKKPVNVPSQNTPDAPDLCFLGSSVESGSASA
                     VVVTTGARTYLGQLASSIAGSSPATSFDRGINQFTWLMIRFILVMVPTVFLLNGFGKH
                     NWLDAFLFAVAVAVGLTPEMLPMIVTVNLSRGAIAMSRKKVIVKRLAAIQNFGAMNIL
                     CTDKTGTITRGKIILERYLDVRGHESERVLHYGYMNSYYQTGLKNLLDEAVLSYGHLE
                     EDLKVKEGYRKIDEIPFDFTRRRMSVIVEEKVNQHILICKGAVDEVMHLSTHAEIEGH
                     VVPLQAEHHAHRTRLVRSLNSQGFRVVAVAYKTMNGDNDEPHYGVADESDLTLLGYLA
                     FLDPPKESASEALDKLAHLNVDVKVLTGDSELVAQYVCNHVGMKTERILLGPEIEKLT
                     DDELASVASGTTLFARLAPVQKERIIRALQKAGNVVGFMGDGINDSPALKAADVSISV
                     DSAVDIAKESSDIILLEQSLLVLETGVREGRRVFGNIIKYIKMAASSNFGNMFSVVGA
                     SAFLPYLPMLPIQVLTNNLLYDFSQTTIPTDDVDNDWLRKPRKWAMGEVQRFILFIGP
                     ISSLFDYATFFLMLYVFDSWQNPALFHTGWFIESIFTQTLIIHVIRTHRIPFIESRAS
                     RPLIVSSILVVSGAAWLTISPLADSLGFVSLPPLYWLCLAGMLVCYVALTQIVKTWFY
                     KRYGE"
     gene            complement(2117..2671)
                     /locus_tag="PLG78_15360"
     CDS             complement(2117..2671)
                     /locus_tag="PLG78_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24551.1"
                     /translation="MQSIKSFFTQQPVAAGAAVLSGLLRLIQVINFSPVGALSIYSGA
                     RIRGLGAFVLPLLVMVVTDWILAGLFYGAGTWTWTTPFVYAAFVLNILLGRLVQKTEN
                     PVAVVGVSLLGTVQFFLTTNLGVWMTTSMYEKTFAGLVNCYVAALPFLRTTLVSDLLF
                     VSAIFLAHHFLAQKFSPRESVVRA"
     gene            complement(2717..>3569)
                     /locus_tag="PLG78_15365"
     CDS             complement(2717..>3569)
                     /locus_tag="PLG78_15365"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HNE24552.1"
                     /translation="FAEQRKQDSAVTALPLLALLGAVSGSSVSCSSLSASTPSGTAAA
                     DRITSAPGDAGSGFFDARCSIDGVRGMGDNQGSTDVFSLAATGSSSVLVLEWNGKKIT
                     NGVGADFIVFENSFNQNGNATTRFMEPVIVEVSYDGSHWCGFNPIYSSGTYSQNPANW
                     TRFAGTTNSYFNQDSNFMNATTLFTSGSSGGGGDQFDLSNLVTGATSGYNCSAVASNS
                     GCAAGDVSQLQGTSSGQGVVYVRMIAATACTNPNAGGATFPQDSGAYGGGPDMDGVIA
                     RNVVSRP"
BASE COUNT          926 a          910 c          910 g          823 t
ORIGIN      
        1 taaatcaagc cgcactcacc ggcgaatcca tgccggttga aaaaaaaccg gtgaacgttc
       61 catcgcagaa cacgcccgac gcgccggacc tttgctttct tggatcgagc gtggagagcg
      121 gatctgcctc cgcggtcgta gtcacaacag gagctcgcac gtatctgggt cagctggcaa
      181 gcagcatcgc cggaagttca ccagcgacga gttttgaccg tggaatcaac cagttcacct
      241 ggcttatgat tcgctttatc ctggtgatgg tgccgacggt ctttttgctc aatggctttg
      301 gcaaacacaa ctggctagac gcattcctgt ttgcggttgc tgttgccgtt ggtctgactc
      361 cggagatgct tccaatgatt gtaactgtga atctttcgcg gggtgctatc gccatgtcca
      421 gaaagaaggt catcgtaaag aggctggccg cgattcaaaa ttttggcgcg atgaacattc
      481 tctgcacaga caagacagga actattacgc gcggaaaaat tattttggaa aggtacctcg
      541 atgtgcgagg acatgaaagc gaacgggttc tgcactacgg atacatgaac agctactacc
      601 agactggact gaagaatcta ttggatgaag cggttctatc ctacggccac ctggaagaag
      661 atcttaaagt aaaagaagga tatcgtaaaa ttgatgagat tcccttcgat ttcacgcgaa
      721 ggcgaatgtc tgtgatcgta gaagaaaaag tcaatcagca catcctgatc tgcaagggtg
      781 ccgtggatga ggtgatgcat ctatccactc acgcagaaat cgaaggacat gttgtccctc
      841 ttcaagctga acaccatgcc cacagaacca gactggttcg atccctgaat tcgcaaggct
      901 ttcgcgtagt tgcagtagct tacaaaacca tgaacggcga caacgatgaa ccgcattatg
      961 gggtagcaga cgaaagcgac ctgactcttc tgggttacct cgcattcctt gatccgccca
     1021 aagaatcagc ttcagaagcg ctcgacaagc tggcacacct gaacgtggac gtcaaagttc
     1081 tcaccggaga tagtgaactt gtggctcaat atgtgtgcaa ccatgttgga atgaaaacag
     1141 agcgcattct gctaggcccg gaaatcgaaa aactcacgga cgatgagctt gcatctgtgg
     1201 catccgggac cactcttttc gcgcgcctcg cacccgtgca aaaagagaga atcatacgag
     1261 cgttacaaaa agcggggaat gtagttggct tcatggggga tgggatcaac gattctcctg
     1321 cgctcaaagc cgcggacgtg agcatttcag ttgattctgc ggtggacatt gcgaaggaat
     1381 cgtcggatat catcttgctc gaacagagtc tgctggttct ggaaaccgga gtgcgagaag
     1441 gtcgtcgagt cttcgggaac atcatcaagt acatcaagat ggcagccagt tccaacttcg
     1501 gcaacatgtt cagcgtggtg ggagcaagcg catttcttcc ctatcttccc atgcttccga
     1561 ttcaagtgct cacaaacaat ctgctctacg atttttcgca gactacaatt ccaactgacg
     1621 acgtagacaa tgactggttg aggaagccgc gaaaatgggc catgggagaa gtgcagagat
     1681 tcattttgtt tattggtccg atcagctcgt tgtttgacta tgctacattt ttcttgatgc
     1741 tgtacgtctt cgactcgtgg cagaatcccg cactgtttca cactggttgg ttcattgagt
     1801 cgatcttcac gcagacactc atcattcacg tgattcgcac acacagaatt ccgtttatcg
     1861 aaagccgcgc cagtcgacct ctgatcgtaa gttccattct agtagtttcc ggtgcagcat
     1921 ggctgacaat atcgcctctg gcggactctc ttggattcgt aagtctgccg cctctctact
     1981 ggctttgtct ggcgggaatg ttagtgtgct atgtggcgct gactcaaata gtcaaaacgt
     2041 ggttttacaa aaggtacggt gagtgaagca gtaggtgaga aggcgcgaag gcaattgcct
     2101 ccgcgcgcta tagaaatcag gcgcgaacga ctgattcgcg tggtgagaac ttttgtgcga
     2161 ggaaatgatg agccagaaag attgctgata cgaaaagaag atcgctcacg agcgtggtgc
     2221 gcaggaatgg aagtgccgca acataacaat tcacaagccc cgcgaatgtt ttctcataca
     2281 tgcttgtggt catccatacc ccgagattcg tagtcagaaa aaactgaact gttccaagga
     2341 gcgaaacacc aactacagca acagggtttt cagtcttctg cacaaggcga ccaagaagaa
     2401 tattcaaaac aaacgccgcg tatacgaaag gtgtggtcca tgtccacgtg cctgcgccgt
     2461 agaaaaggcc tgctagaatc caatccgtta caaccatcac aagcagaggc aaaacgaacg
     2521 caccgaggcc tcgaatgcgc gcaccggaat aaatagatag agcccctacc ggcgaaaagt
     2581 tgatcacctg aatcaggcga agcagcccgg acaatacggc cgcgcctgcg gcgactggct
     2641 gctgggtgaa aaatgactta atgctttgca tacctggtaa tctcctgtgt ttcgttttgc
     2701 gatcaagtaa aaaaggttat gggcgcgaaa caacgtttcg agcgatgacg ccgtccatat
     2761 ctggaccgcc gccataggca ccgctgtcct gcggaaatgt tgccccaccc gcatttggat
     2821 tcgtgcatgc ggtagcagca atcatgcgaa cgtacaccac tccttgtccg ctcgacgtac
     2881 cttgcaattg tgagacatct cccgccgcgc acccggaatt ggacgctact gcggagcaat
     2941 tatacccgga tgtggccccg gtcacgaggt tgctaagatc aaactggtct cccccgcctc
     3001 cggacgaccc cgaagtgaaa agagtggtag cattcatgaa gttgctgtct tgattgaaat
     3061 agctattggt cgtacctgca aatctggtcc agtttgcagg attttgactg tacgttccgg
     3121 aactgtatat cggattaaaa ccacaccagt gacttccatc ataactgacc tctacgatga
     3181 ccggttccat aaagcgggta gtggcatttc cgttctggtt aaatgaattc tcaaagacga
     3241 tgaagtccgc gcccactccg ttcgtaatct tttttccatt ccattccagc accagcactg
     3301 aactactccc ggttgccgcc agggaaaaaa cgtccgtgga tccctgattg tcacccatgc
     3361 cgcgcacacc gtcaatagaa caccgagcat cgaaaaagcc ggaaccggca tctcctgggg
     3421 cagacgttat gcgatcagca gctgcagtcc cggatggcgt tgacgcactc aacgatgaac
     3481 atgaaacact ggagccgctg accgccccga gcaaagcaag gagcggtagt gccgtcacag
     3541 cgctatcttg cttgcgctgc tctgcaaat
//
LOCUS       DAOQJL010000418         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16610_length_3564_cov_3.675691, whole genome shotgun sequence.
ACCESSION   DAOQJL010000418 DAOQJL010000000
VERSION     DAOQJL010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16610_length_3564_cov_3.675691"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="rplS"
                     /locus_tag="PLG78_15370"
     CDS             complement(<1..382)
                     /gene="rplS"
                     /locus_tag="PLG78_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNE24553.1"
                     /translation="MSEVKDETQTETAEAPATPKQVEKIALTVGGGVIYTSLPGLKPK
                     VHPEFNVGDTVRVHYRIKEGDKERVQIYEGTVASIRGEGLARTFLVRRISHDIGVERI
                     FPYESPSIAKIEVMRKGKVRRAKLF"
     gene            complement(369..1037)
                     /gene="trmD"
                     /locus_tag="PLG78_15375"
     CDS             complement(369..1037)
                     /gene="trmD"
                     /locus_tag="PLG78_15375"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNE24554.1"
                     /translation="MNFQVLTLFPERYEAYLNSGLPARAQAKGLFKIDPVRLRDYADP
                     GRGGRVDDTPYGGGPGMLLEVGPIDRALKARVEKSPVILFSPRGKRLDQRMVERYAGL
                     PGLTLVCGYYEGVDERVSEHLVNEIVSLGDFVLGSGDLAALCLIEAVTRLLPGYMGSD
                     ESGVEESSGPEGLLEYPQYTRPRDYQGWTVPEVLLSGDHARIREWRRQQQELITRQRE
                     NERS"
     gene            complement(1037..1441)
                     /locus_tag="PLG78_15380"
     CDS             complement(1037..1441)
                     /locus_tag="PLG78_15380"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNE24555.1"
                     /translation="MRPGEDPLAPLQPDFIPDLKPATMDAMRPTKDGASARIVCDLEV
                     DLTGSWVYAARELWAEKDSVYVYEILGARIVDSEGKDRGEVKDYFETGSTGVLTVDSG
                     GKVVMIPSIDQFVDYSRLKEGIIIVPTFDDFL"
     gene            complement(1554..1790)
                     /locus_tag="PLG78_15385"
     CDS             complement(1554..1790)
                     /locus_tag="PLG78_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNE24556.1"
                     /translation="MQASILLEYIIEALVTKKDEIKIEEIPGPEESVLEVRVADSDVG
                     KVIGKNGSVARSLRQVLLAAGTKDKKNYVLEIID"
     gene            complement(1792..2031)
                     /gene="rpsP"
                     /locus_tag="PLG78_15390"
     CDS             complement(1792..2031)
                     /gene="rpsP"
                     /locus_tag="PLG78_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNE24557.1"
                     /translation="MVKIRLQRYGTKKRPFYRLVAAHSTSRRDGRFIEQIGFYDPVSR
                     VEPLRLETERVKHWISKGAIPSQTVESLLKKAGAI"
     gene            complement(2061..2714)
                     /gene="rpe"
                     /locus_tag="PLG78_15395"
     CDS             complement(2061..2714)
                     /gene="rpe"
                     /locus_tag="PLG78_15395"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNE24558.1"
                     /translation="MKISPSILAADMLDLKTTLRAMDPSAIDFIHMDVMDGHFVPQLS
                     FGERITELAAQHTKIPLDVHLMVDQPELEVPKYFKLKPHNITFHLEATNFPVRLAREI
                     KSAGILPGIALNPGTSVSLLENVLDEIGMVLIMSVEPGFYGQKFLELAIPKVEQLKKM
                     IGSRDILIEADGGIGEGNIGRLARAGVDIVVAGSSCFAPPDSPSDRARALKSAALNG"
     gene            complement(2711..>3564)
                     /locus_tag="PLG78_15400"
     CDS             complement(2711..>3564)
                     /locus_tag="PLG78_15400"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNE24559.1"
                     /translation="YIDVHNDLARMQIRSVIQKKSYPDKPAGLILAQSIDPGAKVSVR
                     EKLYIVVNQPEPVLEMPELVGASYENAKAKLASMPADEEVYALQIGSVSFVPAPNVPA
                     GTVIAQYPAAKERVSPFERAYLLVAQSETAKDMKDAGPIPKQGLLDTIPGQNIAIASQ
                     FFVTLKKEYRIRSIKSPASFVESGVVQSAEEKDGVVYLDVLYKKPSERFQNGLEKISV
                     ELDEPGVCKGELIRGNETSQFFLTKDRKAAEKTETPLVFYRQGTVQVKVTCGDSVVYK
                     KKFHPEV"
BASE COUNT          781 a          955 c          881 g          947 t
ORIGIN      
        1 agaatagttt cgcgcgacgc actttacctt tgcgcataac ttcaatcttc gcgatggatg
       61 gagactcgta agggaagata cgctctacac caatgtcgtg tgaaatcctg cgcaccagga
      121 atgttctggc aagaccttca ccacgaatcg acgcaaccgt tccttcgtag atctgaacgc
      181 gctctttatc gccttctttg attctgtagt gcacgcgcac agtgtcaccc acattgaatt
      241 caggatgtac ttttggtttg aggcctggca ggctggtgta gatgactccg ccgcctacag
      301 taagtgcaat tttttccact tgctttggag ttgctggcgc ttctgccgtt tctgtttgtg
      361 tttcgtcttt aacttcgctc attttctctc tgcctcgtaa tcagttcttg ttgctgccta
      421 cgccattcgc gaattcgcgc atgatcgccc gagagcaaca cctcgggaac agtccatcct
      481 tgataatctc ttggtcttgt gtattgcgga tattcgagca gaccttccgg gccactgctt
      541 tcctccacgc cactttcatc tgatcccatg taaccaggga gcagcctggt gacagcctca
      601 atcaaacaga gtgcagccag atcaccagaa ccaagaacga aatcacccag tgaaacaatt
      661 tcattcacca gatgctcgga aactctctca tccacgcctt catagtagcc gcaaacgagt
      721 gttagaccgg gcagccctgc gtatctctcc accattctct ggtcgagcct cttgcctctg
      781 ggagaaaaca gaatgacggg agacttttca accctggctt tcagagcccg atcaattggc
      841 cccacttcca ggagcatccc gggacctccg ccatacggcg tgtcgtccac tctgcctccc
      901 ctgccgggat ctgcatagtc ccgcaagcgt acagggtcga ttttgaacaa acctttcgcc
      961 tgggctctgg cgggcaagcc cgaattcagg taagcctcgt agcgctcagg aaagagagtg
     1021 agcacctgga aattcatcag agaaaatcgt caaaggtagg aacgatgatg attccttcct
     1081 tgagcctgga gtaatccacg aactgatcta tgctgggtat catcacaacc tttccgccgg
     1141 aatctacagt gagaacgccg gtggagcctg tttcgaagta gtctttgact tctcctctgt
     1201 ctttcccttc cgaatccacg atgcgtgcgc ctagaatttc ataaacgtaa acggaatcct
     1261 tctcagccca gagttcccgg gcggcataca cccaggatcc agtcaggtcg acttcgaggt
     1321 cgcagacgat ccgagcagaa gcgccgtcct tcgtgggtcg catcgcgtcc attgtggccg
     1381 gttttagatc cggtatgaaa tccggctgga ggggtgcgag tggatcttct ccaggacgca
     1441 tcaagtgaat gctgattttg cacggaccac gaagcctgga aaggccgtcc ccgcgacttg
     1501 tgatccggta gatgtcccgg ccaacgtttt tctgaattcg accgaccgga aggtcagtcg
     1561 atgatttcga gtacgtaatt ctttttgtcc ttggttccgg cagcgagaag tacctggcgc
     1621 aaagagcgcg ctacgctgcc gttttttcca attaccttac ccacatcgct gtctgcgacg
     1681 cgcacttcga gcacggattc ttccggtccc ggaatctcct cgattttgat ctcgtccttc
     1741 tttgtgacga gagcttcgat gatgtattcg agcagaatgg aggcctgcaa cttagatagc
     1801 tccggccttc ttgagcaggc tttctactgt ctggctgggg atggcaccct tgctaatcca
     1861 gtgcttcaca cgctctgttt ccagacggag aggctctaca cggctcactg gatcataaaa
     1921 accaatttgt tcgatgaatc tgccgtcgcg gcggcttgtt gaatgagcgg ctaccagacg
     1981 gtaaaatggc cgtttcttgg tgccgtatct ctgaagtcgt atctttacca caggtctatt
     2041 ttctgcgcct gcgtcttatg tcaaccattt agagccgcag acttgagcgc acgagctctg
     2101 tcagaggggg agtccggagg agcaaagcaa gaagatccgg ccactactat gtccactcct
     2161 gcccgggcca gcctgccaat gttgccctcc ccgattccgc cgtctgcctc aatgagtatg
     2221 tctctcgagc ctatcatctt cttcagctgt tcgacctttg gaatggccag ttccaggaac
     2281 ttttgaccat agaagcctgg ctccacgctc atgatgagaa ccataccaat ctcgtccagg
     2341 acgttttcca gaaggctcac agacgttcca ggattcagtg caatgcctgg caggattcca
     2401 gcagacttaa tttcgcgcgc gaggcgaacg gggaagttgg tagcttccag atgaaacgtg
     2461 atgttgtgag gcttgagctt gaaatacttg ggaacttcta gctccggctg atccaccatc
     2521 aggtgaacat ccagaggaat ctttgtatgc tgtgctgcaa gttccgttat gcgctctcca
     2581 aaagagagtt gtgggacgaa gtgtccgtcc atgacatcca tgtgaatgaa atcaatggca
     2641 gatggatcca tggcgcggag agtggttttt aaatccagca tgtccgcggc caggatggaa
     2701 ggagagattt tcatacttct ggatgaaatt tctttttata aacgaccgag tcgccgcagg
     2761 tgactttcac ctgcacggtt ccctggcgat aaaacacgag tggtgtctct gtcttttctg
     2821 ctgctttgcg atcttttgtc aaaaagaact ggctggtttc gttcccgcga atgagttcgc
     2881 ccttgcaaac tcctggctca tcgagctcga ctgaaatctt ttccaatcca ttctggaatc
     2941 gttcacttgg ttttttgtag agtacatcta aatagacaac gccatctttt tcttctgcac
     3001 tttgcactac gccggattcc acaaaggaag ctggcgactt gatgctccgg attctgtatt
     3061 cttttttaag cgttacaaag aactgcgaag caatggcaat gttctgtcca ggaatcgtgt
     3121 cgagcaatcc ttgctttgga atgggacctg cgtccttcat gtcctttgcg gtttcagact
     3181 gcgccacgag tagatatgct ctttcaaaag gagaaactct ctccttggcg gccgggtatt
     3241 gtgcgatcac agtgccggcc ggaacattcg gtgccggaac aaaactcaca gatccaattt
     3301 gcaatgcgta gacttcttcg tctgcaggca tacttgcgag ctttgctttc gcgttctcgt
     3361 acgaagctcc cacaagttca ggcatttcta gaactggttc gggctggttg accacgatgt
     3421 agagtttttc gcgcacgctg actttggcgc caggatcaat gctctgcgcg aggatgaggc
     3481 cggccggttt gtcaggataa ctcttcttct ggatcacaga acgaatctgc atacgagcaa
     3541 gatcgttatg aacatctatg tagt
//
LOCUS       DAOQJL010000419         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16632_length_3561_cov_4.235596, whole genome shotgun sequence.
ACCESSION   DAOQJL010000419 DAOQJL010000000
VERSION     DAOQJL010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16632_length_3561_cov_4.235596"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..938)
                     /gene="cysD"
                     /locus_tag="PLG78_15405"
     CDS             complement(48..938)
                     /gene="cysD"
                     /locus_tag="PLG78_15405"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNE24560.1"
                     /translation="MIDHLRQLEAEAIYILRETAAQFERPALLFSGGKDSITLVHLSL
                     KAFRPSRFPYPLVHIDTGHNFPEAITYRDEMAARIDEKLIVRNVQDSINRGTAIEEKG
                     RFASRNTLQTVTLLETIEEFRFDACIGGARRDEEKARAKERIFSVRDSFGQWDPRLQR
                     PELWNIYNGKIQPGENVRVFPLSNWTELDVWNYIRLEQIPIPNLYFTHKRQVFEKDGI
                     LFPVSDFIKLEKEPVSETEVRFRTVGDMTCTAAVASRASSLDEIIEEIKASNTTERGS
                     RLDDKRSESAMEDRKRSGYF"
     gene            complement(935..2605)
                     /locus_tag="PLG78_15410"
     CDS             complement(935..2605)
                     /locus_tag="PLG78_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HNE24561.1"
                     /translation="MELSKNEKLKQDSGFLRGSIVEELKNSSASFEKDTTLLLKFHGM
                     YQQDNRDARVKTEEGGTEKAHSLMIRGRIPGGRLNTEQYLVWDELASRVGNGSMRLTT
                     RQSIQLHGILKQDIKRTLQKIHESLLSTTGACGDIVRNVTQSLNFLNDPSLAKLDGVA
                     QLISDHFRARSGAYAEVWLDDEKLPGPVEEPIYGDRYLPRKFKIGVTAAGNNSIDIYT
                     NDMGFAATFENGQINGYFVFAGGGLGRTHRNAKTYARVADNLGWIPASKLIPVAEAIV
                     TIHRDFGDRNDRKHARLKYVVAEKGIDWMRTETESRSGAKFEQKPLPAWNTPEYLGWQ
                     KAKDGTLTLGLHILAGRIVDNAGHSLKTSLRNVIRDYRLSTQVTPDQDLILLGIRESD
                     RPAIEQVFRAAGVRMDAPNSLFSKALACPALPTCSLAIAESERTLPAFLEVIQKKLEK
                     YGLEDRAPTVRMTGCPNGCARPYSAELGFAGRSMNIYGVYVGGDYEGTRITKELSDNV
                     PLSEMEGVIDRLFAMWKEQAPSDRFGDFTHRMPLEELQKALPAVRPAQ"
     gene            2695..3408
                     /locus_tag="PLG78_15415"
     CDS             2695..3408
                     /locus_tag="PLG78_15415"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNE24562.1"
                     /translation="MDLSALNLELAALSTEEVVRKLYALDPAGTVLACSASIEDTVLL
                     HMLAEAAKSAGRKPRAFVLDTGRLPEETYQVLEDCRQKYHFEFEMVFPESQDVEKLLR
                     AKGPFSFYESIENRKECCHIRKVLPLKRALSGATLWITGMRREQSVTRADVPFVELDQ
                     AHGGILKANPLALWTEKMVYDYASAHNVPVHPLHRRGFPSIGCEPCTRAIQPGEDFRA
                     GRWWWENADQKECGLHVKS"
BASE COUNT          792 a          866 c          909 g          994 t
ORIGIN      
        1 ggctaagaat cttaaaattt ccatactatt ttcctgattg cctggaccta aaaataaccg
       61 gagcgctttc gatcttccat ggctgactcg gatcgtttgt cgtcgagtct cgatccacgt
      121 tccgttgtgt tgctcgcttt aatctcttcg atgatctcat ccagagaaga agcgcgcgaa
      181 gccacggcgg ccgtgcacgt catatctccg acagtgcgaa agcggacttc cgtttctgac
      241 acaggctctt tttcgagttt tataaagtct gaaacaggaa acaggattcc atctttttca
      301 aagacctgtc ttttgtgcgt gaagtaaaga ttcggaatcg ggatttgttc caggcgaatg
      361 tagttccaaa cgtcgagttc cgtccagttg ctaagaggaa acacccggac gttttcaccg
      421 ggttggattt ttccattgta tatgttccat agttctggac gctggagacg cggatcccat
      481 tgcccaaacg aatcgcgcac agagaaaatt ctttcttttg cccgggcttt ttcttcgtct
      541 cgtctggctc cgccaatgca cgcatcgaat ctgaattctt cgatggtttc gagcaaggtc
      601 acagtctgta gagtattgcg gcttgcgaat ctgccttttt cttcaattgc ggtgccgcga
      661 ttgatgctgt cctgtacatt tctcacgatg agcttctcat caattctcgc ggccatttcg
      721 tcgcgatatg tgattgcttc agggaaattg tgtcctgtgt caatatgcac aagcgggtat
      781 ggaaatctag aagggcgaaa cgctttgagt gaaagatgaa ccagagtgat ggaatctttt
      841 ccacccgaga ataaaagcgc cggacgttcg aactgggccg cagtttcgcg cagaatgtag
      901 attgcttccg cttcgagttg tctgagatgg tcaatcattg tgcgggcctc acggcaggaa
      961 gtgctttctg caattcttct agaggcatac gatgagtaaa gtcaccgaat cgatcggaag
     1021 gagcctgctc tttccacatg gcgaagagtc tgtctatgac cccttccatt tccgagagcg
     1081 gaacattgtc tgaaagttct ttcgtaattc tggttccttc gtagtcccct cctacataaa
     1141 ctccataaat gttcatactt cttccagcaa atcccagttc tgccgagtaa ggacgcgcac
     1201 aaccattcgg gcatccagtc attcgcacgg taggagctcg atcctcgaga ccgtatttct
     1261 ctaatttctt ctgaatgact tcaaggaacg cgggcaatgt gcgttctgac tctgcgatag
     1321 caagactgca cgttggaagc gcaggacagg ccagggcttt tgagaagagg ctatttggtg
     1381 cgtccatacg cacgccagcg gcgcgaaaca cctgttcgat ggctggcctg tcagattcac
     1441 gaataccaag caggatgaga tcctgatccg gggtgacttg agtggagagg cggtaatcgc
     1501 gaataacgtt tctcagcgaa gtcttcagtg aatggcccgc attgtctacg attcttcctg
     1561 ctagaatgtg cagcccgagc gtgagagtgc cgtctttcgc tttttgccag cccaggtatt
     1621 caggtgtgtt ccatgccggc agtggtttct gttcgaattt tgcgccggat ctgctttcgg
     1681 tttccgttcg catccagtcg ataccctttt ctgcgaccac gtacttcagg cgtgcgtgct
     1741 ttctatcgtt gcgatctccg aagtctcgat gaatggttac gatggcttcc gccacaggaa
     1801 tcaattttga tgcgggaatc catccgagat tgtctgcgac tctcgcgtat gttttcgcat
     1861 tgcgatgagt tcggccaagt ccgcctcccg caaacacaaa gtatccattg atttggccat
     1921 tttcgaaagt ggccgcgaat cccatatcgt tcgtatagat gtcgatgctg ttgttgcccg
     1981 cagccgttac accaattttg aattttctcg gtaggtaacg atctccatag attggttcct
     2041 ctacgggccc tggtagtttt tcgtcatcca gccagacttc tgcgtatgcg ccagatcttg
     2101 ctctgaaatg atcggagatg agttgagcta ctccatccag tttcgcaagg gaaggatcgt
     2161 ttagaaagtt taacgattgt gttacattgc gaacgatgtc gccgcaggca ccggtggtgg
     2221 aaaggagcga ttcgtgaatc ttttgtaacg tgcgtttgat gtcctgcttg agaatgccat
     2281 gaagctgaat cgactgacgg gtggtaaggc gcatggatcc attgcctacg cgtgatgcaa
     2341 gctcgtccca gaccaggtat tgctctgtgt tcagccttcc acccggaatt cttccgcgga
     2401 tcatcagaga atgggctttt tctgttccgc cttcttctgt ttttacgcgc gcatcgcgat
     2461 tgtcttgctg gtacattccg tggaatttga gcagcaatgt tgtatctttt tcaaaggaag
     2521 cactggagtt tttcagctcc tctactatgc tgcctcttag gaagccagag tcctgcttga
     2581 gtttttcgtt cttagataat tccatggaaa tctctctgtg aggccatctt tttcatttga
     2641 ccctgacagg acaaacgatt ttagtattca tatcaaatta ctaggaaata agttttggac
     2701 ctttctgctc taaatttgga actcgctgcc ctgtccactg aagaagtggt tcggaagctt
     2761 tatgccctgg acccggctgg caccgtcctc gcctgcagtg cgagcataga ggacacagtc
     2821 cttttgcata tgctggcgga ggcggcaaag tcggcaggaa ggaaaccgcg tgccttcgtg
     2881 ctcgatacgg ggagattgcc cgaagaaacc taccaggtgc tggaagattg caggcagaag
     2941 taccactttg agttcgaaat ggttttcccg gaatctcagg atgtggaaaa gcttttgagg
     3001 gcaaagggcc ctttttcctt ttacgagagc attgagaatc gcaaggaatg ctgtcatatt
     3061 cgcaaggttc ttccattaaa gagagcattg tccggcgcca ctctctggat tacagggatg
     3121 aggcgggagc aatccgtaac gcgggcagat gttccatttg tggaactgga ccaggcgcat
     3181 ggaggcattc tcaaagcaaa ccctctggct ctgtggactg agaaaatggt ctacgactac
     3241 gcaagtgcgc ataacgttcc agttcatcct ctccatcgtc gcggatttcc aagcattggt
     3301 tgtgagccat gtacgcgggc catccagccc ggcgaagact ttcgcgcagg acgatggtgg
     3361 tgggaaaacg cggatcaaaa ggaatgcgga cttcacgtta agtcctgatt tatgagttca
     3421 aaactctttc ccctggtgct ggaccttcac aatcgtccgg tgctcattgc aggagaaggg
     3481 agtcatgccc tggagaaaat tcgccttctg attgagtcgg gcgcacaggt tcgtctctat
     3541 tgtgaaccca atgatcccaa t
//
LOCUS       DAOQJL010000420         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16659_length_3557_cov_3.093375, whole genome shotgun sequence.
ACCESSION   DAOQJL010000420 DAOQJL010000000
VERSION     DAOQJL010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16659_length_3557_cov_3.093375"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLG78_15420"
     CDS             <1..280
                     /locus_tag="PLG78_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HNE24563.1"
                     /translation="LEILGSLYEQGNAIRGIFSQSIPMEDILVSVEGDRDPRLGGLTV
                     ITASYKMGEKRIGSLGVVGPNRMDYVRVVSVVEYIRRLLSSMITRMSN"
     gene            282..893
                     /gene="grpE"
                     /locus_tag="PLG78_15425"
     CDS             282..893
                     /gene="grpE"
                     /locus_tag="PLG78_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNE24564.1"
                     /translation="MENEVMEETQNEETLEGVDVAAGSPDQKEQPASESGSVNLELEK
                     AKAEAMDMRDSWNRERAEFQNYKKRMIHEMGRARSQAVKSFVSGLLPVIDNLNQVLLA
                     KSEDPAVKNFVIGVEMIRSEFLKVLNKENIQSFRPVDQAFDPMRMEAVALDESESVDS
                     PKVVEVYEDGYSLTIDGQEEILRPARVRVLKPAKKKEEANENA"
     gene            956..2887
                     /gene="dnaK"
                     /locus_tag="PLG78_15430"
     CDS             956..2887
                     /gene="dnaK"
                     /locus_tag="PLG78_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNE24565.1"
                     /translation="MAKDKIIGIDLGTTNSCVAIMEGGEPIVIQNAEGSRTTPSIVGF
                     TDKEARLVGQVAKNQAITNPTNTVRSIKRFMGRRFSEVQDEMKVVPYKVIRSGTDGIK
                     VETDQGSFTAQEVSAIILQKMKKTAEEYLGHPVTKAVITVPAYFNDEQRQATKDAGRI
                     AGLEVERIINEPTAAALAYGLDKKTKNMKIAVYDLGGGTFDVSILELGEGVFEVKSTN
                     GDTHLGGDDFDQAIMQWMMDEFKKESGIDISKDRMALQRLKEAAEKAKIELSGTLQSQ
                     INIPFITADASGPRHMNMTLSRAKFDQLTKSLVERTRMPCENAIKDAGLSAKEIDEVI
                     LVGGSTRIPAVQELVKQLFGKEPNRSVNPDEVVAVGAAIQGGVLAGEVTDVLLLDVTP
                     LSLGIETLGGVFTRLIERNTTIPTRKSQIFSTAADNQTAVSVHVMQGEREMAKDNRTL
                     ARFDLLGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEHKIRVETTSGLTEDEI
                     KKMVREAQENEARDKEQRKLVEAKNEADSLTYSLERALQDAGDKVNPADKAAAEDLIK
                     RTREALESGDAERIFQARDEISKGSMDLGQKIYAASQQPGAEGGPGPGAQAGGQPGGE
                     ERGEKVVDADYTVVDEDKK"
     gene            2900..>3557
                     /locus_tag="PLG78_15435"
     CDS             2900..>3557
                     /locus_tag="PLG78_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE24566.1"
                     /translation="MADRDYYEILGVARTATKEEIKKAYRKLAIQFHPDKNKGDKAAE
                     EKFKEATEAYEVISDDQKRAAYDQYGKAGVGQGGGPGFGYKAYTDFSDIFGDIGDIFS
                     EFFGGQGGGFAGGGRVRGARRGSDLRYNVEISLEEAASGKEIKIEIPRQEACDDCGGS
                     GAKKGTQASVCPLCQGAGQVRRTQGFFSVTTTCPQCQGQGRVIASPCATCAGSGLVEK
                     K"
BASE COUNT         1051 a          769 c          990 g          747 t
ORIGIN      
        1 tctggagata cttggttctc tctatgagca gggaaatgcg attcgcggaa ttttttcgca
       61 gagcattcct atggaagata ttttagtcag cgtggaagga gaccgtgatc cgcgtctggg
      121 cgggcttacg gtcattactg caagttataa aatgggagaa aaacgaatcg ggtctctggg
      181 tgtggttggt ccaaacagaa tggattacgt tcgagtagta agcgtggtgg aatacatccg
      241 cagactcctg tccagcatga ttaccaggat gagcaattga tatggagaat gaagtaatgg
      301 aagaaacgca aaacgaagaa actctggaag gcgtggatgt tgctgccggt tcgccggatc
      361 aaaaagaaca gccagcgtct gaaagcggaa gcgtgaatct ggagcttgag aaagcaaaag
      421 cggaagctat ggacatgcgc gattcctgga atcgcgaacg tgctgagttc cagaactaca
      481 aaaagcgtat gatccatgag atgggccgcg ctcgttcgca ggctgtaaag agcttcgtaa
      541 gcggactact tcctgtgatt gacaatttga accaggtcct tcttgcgaaa tcagaagatc
      601 cggctgtgaa gaatttcgtg atcggcgtag aaatgatccg aagcgaattt ctcaaagtgc
      661 tgaacaaaga gaacattcag tcctttcgcc ctgtggatca ggcatttgat ccaatgcgca
      721 tggaagcagt ggcgcttgat gagagcgaaa gtgtagattc tccaaaagtg gtcgaagtat
      781 acgaagatgg ttattcttta acgatagacg gtcaggaaga gattcttcgt ccggcgcgag
      841 tcagagtgct caagcctgcg aagaagaaag aagaggcaaa tgaaaatgct tgaacagttt
      901 agaaattttg ttaatataag cgagcaatcg cgcaattatt gagaggagaa aggaaatggc
      961 aaaagacaaa atcattggaa tcgacctggg tacaaccaac tcatgcgtcg caatcatgga
     1021 aggcggtgaa ccaatcgtca ttcaaaacgc ggaaggatct cgcacaacgc cgtctatcgt
     1081 cggttttact gacaaagagg caaggctggt gggtcaggtg gcaaaaaatc aggccatcac
     1141 taacccgact aacactgtac gttccatcaa gcgcttcatg ggaagacgct tctctgaagt
     1201 tcaggatgaa atgaaggttg ttccatacaa agtaattcgc tctggaacag acggaatcaa
     1261 agtagaaaca gaccagggct ctttcactgc tcaggaagtt tctgcgatca ttctacagaa
     1321 gatgaagaaa actgcagagg aatacctggg tcatccagtc accaaggctg tgatcacggt
     1381 tccagcttac ttcaatgatg agcagcgtca ggctaccaag gatgcaggaa gaattgcagg
     1441 cctggaagta gaacgcatca tcaacgaacc aactgcggcc gctctcgcct atggtcttga
     1501 taagaagacc aagaacatga agattgcagt gtatgacctg ggcggtggaa cgttcgacgt
     1561 atccattctc gaacttggag aaggcgtatt cgaagtaaag tctaccaacg gggacactca
     1621 cctgggcggt gacgactttg atcaggccat catgcaatgg atgatggacg aattcaaaaa
     1681 agagagcgga atcgatatct caaaagatag aatggctctg cagcgattga aagaggctgc
     1741 tgaaaaagca aagattgaac tgtctggaac tctccagtca cagatcaaca ttccattcat
     1801 cactgcagat gcaagcggtc ctcgtcatat gaacatgact ctgagccgcg cgaagtttga
     1861 tcagctcacc aagtctcttg tagaaagaac tcgcatgcct tgcgaaaacg caatcaagga
     1921 tgcaggcctc tctgcgaagg aaattgacga agtgattctt gtgggcggct ccactcgtat
     1981 ccctgctgtt caggaactgg taaagcagct ttttggaaaa gagccaaatc gctctgtgaa
     2041 cccggatgaa gtagtggctg ttggtgcagc aatccagggt ggggtactcg caggagaagt
     2101 aacagatgtg cttttactcg atgttactcc gctttctctg ggcattgaaa ctctgggtgg
     2161 agtgttcact cgcctcatag agagaaacac tactatcccg actcgcaagt ctcagatctt
     2221 ctctactgcg gctgacaacc agactgctgt gtccgtgcat gtaatgcagg gcgaaagaga
     2281 gatggcaaaa gacaacagaa cactcgcgcg ttttgacctg ctcgggattc cacctgcacc
     2341 tcgcggtgtt ccacagatcg aagtgacttt tgatattgat gcaaatggca tcgtgcatgt
     2401 gtctgcgaaa gatcttggaa caggcaaaga gcataagatt cgcgtggaaa caaccagcgg
     2461 tcttacggaa gacgaaatca aaaagatggt tcgggaagcg caggagaacg aagctcgcga
     2521 taaagaacaa cgcaagctgg tagaagcgaa gaatgaagca gactctctga catattctct
     2581 ggagcgcgcg ctccaggatg caggcgacaa agtgaatcct gcagacaaag cggctgcgga
     2641 agatctaatc aaacgcactc gcgaagcact cgaatctgga gacgctgaaa gaatcttcca
     2701 ggcaagagac gagatttcaa aaggatccat ggatcttggc cagaaaatct acgctgcttc
     2761 ccagcagcct ggagcagaag gtggtccagg tcctggtgct caggcaggcg ggcagcctgg
     2821 cggcgaagag cgcggagaga aggttgtcga cgcagactac actgtagtag acgaagacaa
     2881 gaagtaattc ggaggaacaa tggcggatcg cgactactat gaaattctgg gcgtagcccg
     2941 gacggcgacg aaagaagaga tcaagaaagc ctatagaaag ctcgcgatcc agtttcatcc
     3001 ggacaaaaac aaaggcgaca aagctgccga agagaaattc aaggaagcca cagaagccta
     3061 cgaagtgatt tcagacgatc agaagcgcgc agcgtatgat caatatggaa aagctggagt
     3121 aggccagggc ggcggacctg gattcggtta caaagcttac acagacttct ccgatatctt
     3181 tggtgatatc ggagatatct tttctgaatt ctttggcggc cagggtggcg ggtttgccgg
     3241 cggcggaaga gttcgcggag ctcgccgcgg atcggatctc agatacaatg tagaaatcag
     3301 tctggaagaa gcggcatctg gcaaagaaat caaaattgaa attcctcgcc aggaagcatg
     3361 cgacgactgc ggtggaagcg gagcaaagaa aggaacccag gcatctgtat gtccgctttg
     3421 tcagggagca ggtcaggttc gcagaacaca gggtttcttc tctgtcacta ctacctgtcc
     3481 gcagtgtcag ggacagggaa gagtcatcgc aagtccatgt gcgacgtgcg ctggcagcgg
     3541 cctggtagaa aagaaac
//
LOCUS       DAOQJL010000421         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16907_length_3524_cov_3.534736, whole genome shotgun sequence.
ACCESSION   DAOQJL010000421 DAOQJL010000000
VERSION     DAOQJL010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_3524_cov_3.534736"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLG78_15440"
     CDS             <1..474
                     /locus_tag="PLG78_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24567.1"
                     /translation="YLALKSYDPAVKSFRAVSDSRSASADQRKEAYRGLAYCLSQSGR
                     SEDQELARSEMNKAVRMDPDDPDMQLMRAGMLMKTGSMVDREKAIEVLQALTAGDLSS
                     DKASQAYNLLGLAFHKNGEYSRALKAFDSAIQLNPQNREAYENQRASANAYERQK"
     gene            518..1468
                     /locus_tag="PLG78_15445"
     CDS             518..1468
                     /locus_tag="PLG78_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24568.1"
                     /translation="MRSTVLRPGFRVRAFAPAAIAISIYSANCASFLLNRSLSFERGR
                     ADLEVKTIPVGKFHFVYTEGGKGEAIVLIHGFAGEKDHWTRMARYLTDKYRVIAPDLP
                     GHGENDRLPEENYSIPEQARRLHDFVHQIGLKRFHLVGNSMGGAISTYYASEYPEDVI
                     TLALFDSAGVKSPVKSELATYLEKGENPLVVKTRDDFDRLTAFTFVDPPYIPGVIKSY
                     FTEKAIANKEFNEKIFSDISKDRGGVEGRLSKIQIRTLILWGDTDRVIHPSSTEVFHK
                     GIKNSEVVIMKACGHGPMIERPEETADHYLNFLNKSASAK"
     gene            complement(1470..2063)
                     /locus_tag="PLG78_15450"
     CDS             complement(1470..2063)
                     /locus_tag="PLG78_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24569.1"
                     /translation="MLNSITNAMHAIALGTYTTLVWFGLRDGKPNYTLLIFMAFMLVF
                     VLKILGMIVHLPWVDRNRSRHNVMWIVISIVLVPLNGFTLAAIGAHPLLLSAGVLFTA
                     ILGALYVKSLYGEDWGNFSYLALAMVGIYALCAAISKAQLRVAWIFLVLSNALWMGLA
                     RVKWLREYKLHNDVYHLALIASSYYLYSTVSLGLWAS"
     gene            2146..2218
                     /locus_tag="PLG78_15455"
     tRNA            2146..2218
                     /locus_tag="PLG78_15455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2179..2181,aa:Lys,seq:ttt)
     gene            2264..2335
                     /locus_tag="PLG78_15460"
     tRNA            2264..2335
                     /locus_tag="PLG78_15460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2297..2299,aa:Glu,seq:ttc)
     gene            2414..>3524
                     /locus_tag="PLG78_15465"
     CDS             2414..>3524
                     /locus_tag="PLG78_15465"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24570.1"
                     /translation="MRWFFLVAVLALCSRVSAAPIQGPWQYVWEKGNPEEIIAGRVAF
                     ETTQRPSQIPGRHGDIVWLVHSFDSSAGKTIYAANVSQLLEVYANGRLIYKFGSIDPI
                     ARSSYRGNPFHMFDLPDGFKGDVYFRVYSSGDVIGLRGNLSQGDRSDHIIALFRNDLA
                     RLLLGLCFILMALVGALLAFTDQTEAHKAFASFCFSVGVFVVTTTRIKHLMDVDALVW
                     SYLNLASIMLMPAFFALFLRSMFSLNRLWNRITLIIPYSLFLFLAAGVLLCLAGFAQI
                     IDFLTPFNVAALICAVSSLAFVVREAFRKSPDARVMIGGGVMMAIAATHDIALESFGF
                     FPSTGIWLHWGMLCLILTLAFLVRKRYVEMFGRYQK"
BASE COUNT          859 a          832 c          925 g          908 t
ORIGIN      
        1 tatctggctc tcaagagtta tgatcctgct gtgaaatcgt ttcgcgctgt ttctgattcc
       61 agaagtgcaa gtgcagatca gagaaaagaa gcataccgcg gtcttgcgta ttgtttgtct
      121 caatcaggca gatcggaaga tcaagaactg gccagatcgg aaatgaacaa agcagtccgc
      181 atggatccag acgatcctga tatgcagctc atgcgtgctg gaatgctcat gaaaacagga
      241 tcgatggtgg accgggagaa agcaattgaa gtgttgcagg ctctcactgc gggagatctg
      301 tcgagcgaca aagcatctca ggcgtacaat ctgcttggcc ttgcgtttca caagaacggg
      361 gaatactctc gggcactcaa agcatttgac tctgccatcc agctgaaccc tcagaacaga
      421 gaagcatacg aaaatcagcg cgcaagcgcc aatgcgtacg aaaggcagaa gtaaagaaaa
      481 gaaatcgcca tagcgagcgt tcatgacaac gagggtcatg agatctactg ttctccgtcc
      541 aggctttcga gtacgcgcgt ttgctccggc tgccattgca atttctatct acagcgcgaa
      601 ctgcgcttcg ttcttactca atcgtagtct atcttttgaa cgcggccgcg cagatctcga
      661 agtcaaaaca atcccggttg gcaaattcca tttcgtatac acggaaggag gaaagggaga
      721 ggcgattgtt ctgattcacg gatttgcggg agagaaagac cactggactc gcatggcgcg
      781 ctaccttacg gacaagtata gagtcatcgc gcctgatctt cctggacacg gagaaaatga
      841 caggttgcca gaagagaact actctattcc ggagcaggcc aggagattgc acgatttcgt
      901 tcatcagatt ggcctgaagc gatttcacct ggttggaaat tcaatgggtg gtgcgatttc
      961 tacctactat gcatccgagt atcctgaaga cgtaatcact cttgcactgt ttgattctgc
     1021 gggcgtcaag agtccggtga agagcgagct ggccacgtat ctagagaaag gagaaaatcc
     1081 actggtggtg aagaccagag acgattttga tcgtttgact gcctttactt ttgtggatcc
     1141 gccctacatt cctggtgtga tcaagagtta tttcacagaa aaagcgattg cgaacaaaga
     1201 attcaacgag aagattttct ctgacatcag caaggatcgg ggaggggtag agggcaggct
     1261 ttctaagatt caaattcgca ctttgattct ctggggagac acggacagag tgatccatcc
     1321 ttcttccaca gaagtgtttc acaaaggaat caagaattct gaagtagtga tcatgaaggc
     1381 atgcggacac ggccccatga ttgaaaggcc cgaggagacc gcagatcatt atttaaattt
     1441 tctaaacaaa tctgccagcg cgaaatgatt caagaagccc agagtccaag agacactgtg
     1501 ctgtagagat agtagctgct ggcaatcagc gccagatgat agacatcgtt gtggagtttg
     1561 tattcgcgca gccatttcac tcgcgcaaga cccatccata gagcattgga caacaccagg
     1621 aaaatccacg ccactctgag ctgcgcttta gagatggcag cgcacagagc gtagattcca
     1681 accatcgcca gggcgaggta agaaaaattg ccccagtctt ctccatagag agattttaca
     1741 tagagagcgc ccagaatcgc agtgaaaaga actcccgcac tgagaagaag agggtgcgcg
     1801 ccaatggccg cgagagtgaa gccgtttaaa ggaaccagaa caatgctgat gacgatccac
     1861 attacgttgt ggcgacttct gttgcgatcg acccagggaa ggtgcacgat cattcccagg
     1921 attttcagaa cgaacacaag catgaacgcc atgaaaatga ggagagtgta gttgggtttg
     1981 ccgtctctga gcccgaacca gaccagagtg gtgtaggttc caagcgcaat tgcgtgcatg
     2041 gcattcgtga tcgagttgag cattgcagtc agaaaaatgt gtttgcactt cttcgtcaat
     2101 tcacgcttga ccagggatgg tcctggcgaa atctgactcc cagccgaggc gttagctcag
     2161 tcggtagagc atttcccttt taaggaagtg gtcccgggtt caagtcccgg acgcctcata
     2221 ttctgaaagc gtaaggacct ggtccggacg caattggggt aatggcccgt tcatctagcg
     2281 gttaggatac cggattttca ttccgggcac acgggttcga ttcccgtacg ggctactttc
     2341 tctcctcctt gattctttcc catccccggc tcacatcggt tgacatttcc gggcagcctc
     2401 ttctccattc cgtatgcgct ggttcttcct tgtggcggta ttggcgctgt gctctcgcgt
     2461 gtctgcagca cccatccagg gtccgtggca gtatgtgtgg gagaagggca atccagagga
     2521 aatcatcgct ggaagagtgg cgttcgaaac cactcagcga ccttctcaaa ttccaggcag
     2581 gcatggagac atcgtctggc tggtgcattc ctttgattct tctgcgggca aaacgatcta
     2641 cgctgccaac gtttcgcagt tactcgaagt atacgcgaac ggtcgcttga tttacaaatt
     2701 tggatcgatc gatccgatcg ctcgctcttc gtatcgaggc aatccatttc atatgtttga
     2761 tttgccagac ggattcaaag gagatgtgta ctttcgcgtg tattcgtctg gagatgtgat
     2821 cgggcttcgt ggaaacttat ctcagggaga tcgatcggat cacatcatcg cgctttttcg
     2881 aaacgatctg gccaggcttt tactcggcct ttgttttatt ctcatggcac tcgtgggcgc
     2941 gcttctggcg tttacagatc aaacagaagc tcacaaagcg tttgcctctt tttgtttcag
     3001 tgtgggcgtg tttgtagtca caacgactcg catcaagcat ctcatggatg tagacgcact
     3061 tgtatggtct tatttgaacc tggcgtcgat catgctgatg cctgcttttt ttgctctgtt
     3121 tttgcgatcc atgttttcac tcaatcgact ctggaacagg atcactctga tcattcctta
     3181 tagtttgttt ctattcctgg ccgcaggtgt gctcttgtgc ctggccgggt ttgcgcagat
     3241 cattgatttt ctgactccgt ttaacgtggc cgcactgatt tgtgctgttt ctagccttgc
     3301 ttttgtggtg cgagaagctt ttagaaaatc gccggatgca agagtgatga ttggcggcgg
     3361 cgtgatgatg gcgattgcgg ccacccacga cattgcgctt gagtcttttg gtttttttcc
     3421 gagcacagga atctggctcc actggggtat gctctgtttg atcctcactc ttgcgtttct
     3481 cgtgcgaaaa cgctacgtgg aaatgttcgg aaggtaccag aaag
//
LOCUS       DAOQJL010000422         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16952_length_3517_cov_4.734835, whole genome shotgun sequence.
ACCESSION   DAOQJL010000422 DAOQJL010000000
VERSION     DAOQJL010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16952_length_3517_cov_4.734835"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLG78_15470"
     CDS             complement(<1..241)
                     /locus_tag="PLG78_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HNE24571.1"
                     /translation="MNYSAQRLNIVEPSPTLAISAKAAELKAAGRDVIGFGAGEPDFD
                     TPDHIKAAAQKAMAEGQTKYTPVGGTAKLKKAVVDK"
     gene            297..773
                     /locus_tag="PLG78_15475"
     CDS             297..773
                     /locus_tag="PLG78_15475"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNE24572.1"
                     /translation="MHIALYRPEIPPNTGNIARLAVCTDTPLHIVGEPSFSMDEKAVR
                     RAGLDYWAQLDLKRHADYEEFYASLASIGVPLVAFSRFATDSMWDFTFSGQEALLFGQ
                     ETAGLPQEITEHVRKHGRLLRIPVGEKCRSLNLSNSVAIACYEVFRQNRRSFSWAL"
     gene            722..1558
                     /locus_tag="PLG78_15480"
     CDS             722..1558
                     /locus_tag="PLG78_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNE24573.1"
                     /translation="MLRSIPSKSQIFLLGALILTGSCITEEQAARFRPGTRSSREALR
                     PDDFQIQPNYTDEERAVPIMLAPLPDVVPQVFILSDLPPAGNQMRQASGVAFSTGYLA
                     TSYYHRRRGKPGYTCSPSFVYNSLNGGKDEGISVLDALDFLHARGCPTEQLMPFNEKD
                     FFRKPGPDALQNADAFRVRGYSRIDFRDFDQVHAHLLQGSVLIVSMKITENFVDLREP
                     LWTPAGKFVAIHTVGVIGYDITRQEVLIQNSAGGTWGHFGTARMPWIWFQRLTEKAFV
                     LF"
     gene            1617..2765
                     /locus_tag="PLG78_15485"
     CDS             1617..2765
                     /locus_tag="PLG78_15485"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNE24574.1"
                     /translation="MNLSIPKEIVAGEKRVAVVPESVKKFIASGFQVRVEKDAGAAAG
                     FSDEDYKAAGAEIISTAGDLYSQADVLVKVQKPIQHPASGKNELEMLKAGALYVGFFY
                     SLSNADLVKLAATNKVNVLSMDAVPRITRAQRMDALSSQTNLAGYKAVLMAANSLTKI
                     FPLMMTAAGTVQPAKVVIIGAGVAGLQAIATAKRLGAVVEVSDVRPAVKEQVESLGAK
                     YIEPPVQDNLEGQGGYAKEASAEFLRKQQEILGNHIAAADVVITTALVPGKKAPVLVT
                     EEMLVRMRSGSVVVDLAAEQGGNCVWTDPGKTITKNGVTVIGETNVVSHVAFHASQLY
                     SRNVLALMEVVSKEGKIALNLEDEIVKGSLITHEGQIIHEATRAAIGA"
     gene            2782..3084
                     /locus_tag="PLG78_15490"
     CDS             2782..3084
                     /locus_tag="PLG78_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNE24575.1"
                     /translation="MDTATLIASLYVFVLAGFVGFLVITKVPPLLHTPLMSATNAISA
                     ISVVAAIVVAGSHHGSILPSILGCIAVASAMTNVVGGFLITDRMLKMFKKKKEEKK"
     gene            3081..>3517
                     /locus_tag="PLG78_15495"
     CDS             3081..>3517
                     /locus_tag="PLG78_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNE24576.1"
                     /translation="MIGELLQNLLGNAVDLVSLINIVYLVSGVLFILGLKMLGSPESA
                     RQGMFLAEIGMLLAVVGTLIDKEIVSFEWILVGAVVGTALGAVIAIWTPMTAMPQRTA
                     LSHAFGAAAVTLVGVAKFYDHPDMGAIKLSAIGFEVMVGALTFT"
BASE COUNT          832 a          881 c          927 g          877 t
ORIGIN      
        1 atttatctac caccgctttc ttgagcttcg cagttccgcc cactggagta tacttggtct
       61 gaccctccgc catagccttt tgtgctgcgg ctttgatatg atcaggcgtg tcaaagtcag
      121 gttcacccgc accaaagccg atcacgtctc taccggcagc tttgagttct gccgccttgg
      181 ctgaaatagc cagagttgga gagggttcta ctatattaag tctctgcgct gaatagttca
      241 tagttctaat cccaattttg gcgtgcaccc ctcaaggcaa atggtttccg agtggaatgc
      301 acatcgcttt atatagaccg gagatcccgc cgaacaccgg caacattgcg cgactagcag
      361 tgtgcacgga cacgccgctg cacatagtgg gcgagccttc cttctccatg gacgaaaagg
      421 cggtgcggcg ggcaggactg gactactggg ctcaactgga cctcaaaagg catgcggact
      481 acgaggaatt ctatgcgagt ctggcatcga ttggagtgcc cctcgtagcg ttttcgcgct
      541 ttgccacgga ttctatgtgg gacttcacgt tttccggcca ggaagcactc ctttttggtc
      601 aagaaactgc aggattgccg caagaaatca ctgaacatgt aagaaagcat ggtcgcttac
      661 ttcgtatccc ggtaggagaa aagtgccgat ctttaaacct gagcaactct gttgcgatcg
      721 catgctacga agtattccgt caaaatcgca gatctttctc ctgggcgctc tgattcttac
      781 cggatcctgc atcacagaag aacaagcggc ccgctttcgc cctggcactc gctcctctcg
      841 cgaagcgctt cggccggatg attttcagat tcagcccaac tacacagacg aagaaagagc
      901 tgtgcccatc atgctggctc ctttgccaga tgtcgtgccg caggtcttta ttctatctga
      961 tttgcctccc gcaggcaacc agatgcgcca ggcgagcggt gttgccttct ccacagggta
     1021 tctggcgacg agctactacc atcgtcggcg cggcaagcct ggatacacgt gctctccttc
     1081 ctttgtatac aactccctca acggcggaaa agacgagggc atctcagtac tcgatgctct
     1141 agacttttta catgcgcgag gatgcccgac agagcaactc atgcccttca atgaaaagga
     1201 tttcttccgc aagcctggcc ccgatgcgct tcagaacgcg gacgcatttc gcgttcgcgg
     1261 ttattccaga atcgactttc gtgactttga ccaggttcac gcacacttgc tgcagggttc
     1321 tgtcttgatc gtatccatga agatcacgga aaactttgtc gatcttcgcg agccgctctg
     1381 gactccagct ggcaagtttg ttgcgattca cacagttgga gtcatcggat acgacataac
     1441 gcggcaggaa gttctcattc agaattcggc gggtggtaca tgggggcact ttggcacagc
     1501 gcgcatgccg tggatctggt ttcagcgttt gaccgaaaaa gcattcgtgc tcttttgatt
     1561 tgaccccgtt ggaatgcggg ctcatttgtc ctttcaccgt ttacggagag gatgaagtga
     1621 atctttcaat tccaaaagaa atcgtagcag gcgaaaagcg tgtcgccgtc gttccagaat
     1681 cagtcaaaaa attcatcgcc agcggatttc aggttcgtgt ggaaaaagac gcgggggccg
     1741 cggctggttt ttctgacgaa gactacaagg cagccggcgc tgaaatcata tcgacagctg
     1801 gcgatttgta tagccaggca gacgttctgg tcaaagttca aaagccaatt caacatcctg
     1861 cttcgggcaa gaacgaactt gaaatgctga aagcaggcgc actttacgtt ggattcttct
     1921 attcactgag caacgccgac cttgtgaaac tggcagcgac taacaaagtg aatgtgctgt
     1981 ccatggatgc agtgcctcgc ataacacgtg ctcaacgaat ggatgctctt agttcgcaga
     2041 caaacctcgc gggatacaaa gcagtgctga tggctgccaa ttctctgact aaaatttttc
     2101 ctttgatgat gactgccgcg ggaaccgtac aacctgcgaa ggtggtgatc attggagcag
     2161 gtgtagctgg cttacaggca atcgccactg cgaaacgact cggtgcagta gtagaagttt
     2221 ctgatgttcg ccccgcagtg aaagagcagg tagaaagtct cggtgcaaaa tacattgaac
     2281 cgcctgtgca ggacaatctc gaaggacagg gcggttatgc aaaagaagca tctgcagagt
     2341 ttcttcgcaa gcaacaggaa attctaggaa accatatcgc agctgcggac gttgtgatta
     2401 caaccgcgct tgtaccaggg aagaaggcgc ctgttcttgt gacagaggaa atgctcgttc
     2461 gcatgcgttc tggatctgtg gttgtagatc tcgccgcgga gcagggcgga aactgcgtgt
     2521 ggactgatcc cggtaaaacc attacaaaga atggagtgac cgtgattgga gagacgaatg
     2581 tcgtctcgca tgttgctttt cacgcaagcc agctttactc aaggaacgtt ctcgccctca
     2641 tggaagttgt gtccaaggaa gggaagatcg ccttgaacct ggaagatgaa atcgtaaaag
     2701 gcagcctcat cacgcatgag gggcaaatca ttcatgaagc tactcgcgca gcgataggcg
     2761 cgtaaacagg aggattcaca attggataca gcaactctca ttgcaagttt atatgttttc
     2821 gtgctcgcgg gattcgttgg atttctcgtg attacgaaag ttccgccgct gctgcatact
     2881 ccactcatgt cggcaacaaa tgcgatttcc gcaatttctg ttgtggcagc catcgtcgtc
     2941 gcaggatctc atcacggttc cattttgccg agcatcctgg gatgcatcgc tgtggcctct
     3001 gcgatgacaa acgttgtggg cggctttctg atcactgacc gtatgctcaa aatgtttaag
     3061 aagaagaaag aggaaaagaa atgattggcg agctccttca aaatcttctc ggcaatgccg
     3121 tagatctggt cagcttaatt aatatagttt atttagtatc cggagtcctg ttcatactcg
     3181 ggttaaaaat gctcggatca cctgagtccg cccgacaggg tatgtttctg gcggagatcg
     3241 gcatgctgct tgcggtcgtg ggcactctga tcgacaagga aattgtatct ttcgaatgga
     3301 tcctggtggg cgcagttgta ggaacggcgc tgggtgccgt cattgccatc tggactccta
     3361 tgactgcgat gccgcagcga actgcacttt cgcacgcatt cggtgcagct gccgtaactc
     3421 tagtaggtgt ggcgaaattt tacgatcatc cggatatggg agcaatcaaa ctctctgcga
     3481 ttggtttcga agtcatggtt ggtgcgctca ctttcac
//
LOCUS       DAOQJL010000423         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_16963_length_3516_cov_4.308581, whole genome shotgun sequence.
ACCESSION   DAOQJL010000423 DAOQJL010000000
VERSION     DAOQJL010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16963_length_3516_cov_4.308581"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLG78_15500"
     CDS             <1..840
                     /locus_tag="PLG78_15500"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24577.1"
                     /translation="GRDCSVDDPAIASKIDKSKTVVIFVHGLMADEVLWQEPTAGKSG
                     FGPRLDEDGRFCCFYVRYNSGRHISTNGRELAGLLEEFVKCHGKKVKRLVIIAHSMGG
                     LVSRSAGYYGTRAKHKWISLLTNVVLVGVPNDGSYLEKFGHLATFVLKRIWTIPTKII
                     GRIADERSDGIKDLRWGLLVDEDWQHADADNLIAVHRSAVPPLPGVKYCIVAGTLLKE
                     DAPVSLYFGDGLVGRASATGEVFRQADSSEEFLEFRIYPGFGHIRLITADEVFHYLIA
                     RIA"
     gene            complement(881..2293)
                     /gene="lpdA"
                     /locus_tag="PLG78_15505"
     CDS             complement(881..2293)
                     /gene="lpdA"
                     /locus_tag="PLG78_15505"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNE24578.1"
                     /translation="MESFDLAVIGGGPGGYVCAIRAAQLGLKTAVIESNPTLGGTCVN
                     VGCIPSKALLDSSEKYSKALHDFKDHGIQTGSVKIDVAQMMARKDRIVKELTDGLNYL
                     MTKNKITVYRGKGSFVRTEASGTVLAIAGKEGISEITARNVVIATGSEIIRIPGFEPD
                     GETIITSDHAIALKSVPQHLIIIGGGVIGLELGSVWNRLGAKVTVVELLPDILMGMDR
                     QMREMARRTLQKQGIEFLLEHKVVKAEKKKSGVVVSLETTAGEKKSIEGDRLLVAVGR
                     KPYTDGLNSDAVGVKINQRGRIAVDPHTLQTDAPGIYAIGDVIEGAMLAHRAEEEGVK
                     VAELLAGKSGHVNYKALPFIVYTWPEFAWVGQSEEDLQKAGIEYKIGKFLFRPNGRAK
                     AMNEPDGQVKIIADKRTDTILGVFIVGPGASELIAEAVVAVEFGASAEDIARSFHAHP
                     TLSEVMKEAALDVDKRSIHS"
     gene            complement(2300..3481)
                     /gene="odhB"
                     /locus_tag="PLG78_15510"
     CDS             complement(2300..3481)
                     /gene="odhB"
                     /locus_tag="PLG78_15510"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNE24579.1"
                     /translation="MAASIDIKVPPMGESISEATVAGWRKKPGEQVNTGDILVELETD
                     KVTMEVPAPASGVLSKIVKNSGEIVKLGDLLATLEEGAATAAAKPATAAAAPQPAAQS
                     QNTTLAPGAARLASEAGVNPASVAGTGVRGQVRKEDIVNHLEKSTPDVPPGKSRPVST
                     AGREVVQPMSPLRKKIAERLVQAQQTAAILTTFNEVDMQRVMDLRAKYKDAFKEKHGA
                     ALGFMSFFTRAAIEALRAFPAVNAEIRGTDIVYKNYFDIGVAVGGPKGLVVPIIRNAE
                     QLSFAELEKEILRLAVKVKESSISLDDLQGGTFTISNGGVYGSMLSTPILNPPQSGIL
                     GMHNIVKRAVVIDDQIVIRPMMYLALSYDHRIIDGREAVSFLVRIKECIENPERMLLD
                     I"
BASE COUNT          749 a          887 c          911 g          969 t
ORIGIN      
        1 ggccgggatt gctctgtgga tgacccggcc atcgcgagta agatagataa gagcaaaacc
       61 gtagtgattt tcgtgcatgg tctaatggca gatgaagttc tctggcagga gcctactgca
      121 ggcaaatctg gattcgggcc acgtctggac gaagacggtc gattttgctg tttctatgtg
      181 cgctataaca gtggaagaca catttctacg aatggtcgcg agcttgcagg actgctcgaa
      241 gagttcgtga agtgccatgg caaaaaagtg aaacgactcg tcatcattgc tcattcgatg
      301 ggtggtctgg tttcgcgctc tgccgggtat tacggaacac gcgcgaaaca caaatggatc
      361 tctcttctta ccaatgtagt actggtcggt gtgcccaatg acggatccta tctggagaaa
      421 tttggacatc tggcgacttt tgttttgaaa cgaatctgga ctatacccac gaagatcatt
      481 gggcgaatcg cagatgagag aagtgacgga atcaaagatc tcagatgggg attgctcgtg
      541 gatgaggact ggcagcatgc ggacgcagac aatctgattg cagtgcacag gtccgccgtt
      601 ccgcctctgc caggggtgaa gtattgcatc gtggcaggaa cgcttctcaa agaggatgcg
      661 ccagtgagtc tatattttgg agatggcctg gtaggccgcg cgagtgcaac aggagaagtg
      721 tttcgccagg cagattcatc cgaggaattt ttggaattca gaatctatcc gggattcggg
      781 cacatacggc tgatcacggc agatgaagtc ttccactacc tgatcgcaag aattgcctga
      841 tgtgcctgcc gcgtcgcggc ggcactcgcc ctcagggcga tcagctgtga atagatcttt
      901 tgtccacgtc gagggccgcc tctttcatta cctcagatag agttggatga gcgtggaaag
      961 atcgtgcaat gtcttctgca gatgcgccga attccacggc aaccacagcc tctgcgatga
     1021 gctctgaagc gccagggcca acaatgaaca ctccgaggat tgtatctgtt cgtttgtctg
     1081 cgatgatctt tacctggccg tccggttcat tcatcgcttt ggcccgacca tttggacgaa
     1141 atagaaactt tccgatcttg tattcgattc ctgctttttg cagatcttct tctgattgtc
     1201 ccacccatgc gaattctggc caggtgtaca caatgaaagg caacgccttg tagttcacgt
     1261 gaccgctttt tcctgcaaga agttcggcca cttttacgcc ttcttcttcc gcgcgatgag
     1321 cgagcatggc gccttcgatg acatcaccga tggcgtagat gccaggtgcg tctgtttgca
     1381 gagtgtgagg atctactgca atgcggcctc tctggttgat cttcacgccg actgcgtcag
     1441 aattcagacc atctgtgtat ggctttcgcc cgacagcaac gagtagcctg tctccttcaa
     1501 tcgacttttt ctctcctgcc gtagtttcaa ggctcacaac tacgcctgat tttttctttt
     1561 ccgctttgac tactttgtgt tctagcagga attcaatccc ttgtttctga agagtgcgtc
     1621 gcgccatttc gcgcatctgt ctgtccattc ccatgagaat gtccggcaag agttccacca
     1681 ctgtgacttt tgcacccagg cgattccaca cagaaccaag ttcgagacca atgactccgc
     1741 caccgatgat gatcaaatgt tgcggcactg atttgagtgc aatggcgtga tccgaagtga
     1801 tgatggtttc tccgtccggt tcgaatcctg gaatgcgaat gatttctgag cctgtggcga
     1861 tgactacatt gcgagctgta atttcagata tgccttcctt ccctgcaatc gcaagcacgg
     1921 tgccgcttgc ctctgttcgc acgaaagaac cctttcctcg ataaacagta attttgttct
     1981 ttgtcatcag gtagttcaga ccgtccgtga gttccttgac gattctgtct tttcgcgcca
     2041 tcatctgcgc tacatcgatc ttcaccgatc cggtttgaat tccatggtct ttgaagtcgt
     2101 ggagtgcttt ggagtatttt tcggaagaat ccagaagagc tttggaagga atgcagccaa
     2161 catttacgca cgttccgccc agtgtagggt tggattcaat gactgctgtt tttagtccaa
     2221 gctgtgctgc gcgaatggcg cacacgtaac cgccgggccc gccgccaatg actgccagat
     2281 caaatgattc cataagccct cagatgtcga ggagcatgcg ctccggattt tcaatgcatt
     2341 ctttgattcg cacaaggaag ctcactgctt cgcggccatc tatgattctg tggtcgtaag
     2401 aaagtgcgag gtacatcatg ggtcgaatta cgatttgatc gtctatcacc acggctcttt
     2461 tcacgatgtt gtgcatcccg aggattccac tctgcggtgg attgagaatt ggagtggata
     2521 gcatggagcc gtaaacaccg ccatttgaaa ttgtgaaagt gccgccctgc agatcgtcca
     2581 gagagataga gctttctttt actttcacag caaggcgcag gatttctttt tcaagttctg
     2641 cgaaggatag ttgttctgcg ttgcgaatga tcggaacgac gagacctttc ggtccgccta
     2701 cagcgactcc aatatcgaag tagtttttat aaacaatgtc tgttccgcga atctccgcgt
     2761 tgaccgcagg gaatgcgcga agagcttcaa tggctgcgcg agtgaagaaa gacatgaaac
     2821 cgagtgctgc tccgtgcttt tctttgaacg catctttgta tttcgcacgc agatccatga
     2881 ctctctgcat gtctacttca ttgaaagttg tcaggatggc cgcagtttgc tgggcctgta
     2941 cgagtctctc tgcgattttc ttgcgaagag ggctcatggg ttgaaccact tctcttccgg
     3001 ccgtacttac cgggcgcgat tttccaggag gaacatccgg agtggatttc tctaaatgat
     3061 ttacgatgtc ttcttttcta acctgtccgc ggactccagt tccggcaacg cttgcaggat
     3121 tgactcctgc ttcactcgcg agtcgggctg ctccaggtgc aagagtggtg ttctgcgatt
     3181 gcgccgcagg ttgcggtgct gctgcagctg ttgccggttt ggccgccgcg gtggcggctc
     3241 cttcttccag agttgccaga agatcgccta actttacaat ttcgccacta ttctttacaa
     3301 tcttcgaaag tacgccggat gcgggcgcgg gcacttccat agttactttg tctgtttcca
     3361 gttccacgag tatgtcaccc gtgttcacct gttctccggg tttctttctc cagccggcca
     3421 ctgtggcttc tgaaatggat tcgcccatag gcggaacctt gatgtcgatc gaagcggcca
     3481 ttctattctc cctttgcgag gacctggtcc atgatt
//
LOCUS       DAOQJL010000424         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17006_length_3511_cov_4.901620, whole genome shotgun sequence.
ACCESSION   DAOQJL010000424 DAOQJL010000000
VERSION     DAOQJL010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17006_length_3511_cov_4.901620"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLG78_15515"
     CDS             complement(<1..784)
                     /locus_tag="PLG78_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE24580.1"
                     /translation="MKLEDRYQSLFEKAPLGFFVADRQGNIEATNSTLRSLFSLEKKK
                     KPNLLTERHLANAGFATQFRLCISRAEPVIAEADFRIKTGYRYCVQYFLTPFFDRGRV
                     VGVQGVVINQTARMEALEEFQRIHDYNSRLLSSLSPVVSVGPDLRIRFANDSFAQWAG
                     ISSEKLMNRLLVPVLKLKGEERDAFAANVREAGKSHKTRKGLQFRAGKSTFGYSLFPF
                     EKDSTGIIMRDISREKELEIRIQKLNGRLIELQEEERQRIAGD"
     gene            complement(844..2082)
                     /locus_tag="PLG78_15520"
     CDS             complement(844..2082)
                     /locus_tag="PLG78_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HNE24581.1"
                     /translation="MKRILLLCMLAFSSCTREDPNYVWLLSLAAGGQTPPSGETSVTV
                     DVSDVTGSPDFLFDTTRTIPVFITVRDPVPQGSGTLVQILDSSSAQELLFQATSNAQG
                     SVEGTFTVNQTTKLVTLRVTIGTDVHTWTINITDVQEIRRFLFVTGEVNMTLVADADS
                     DGVPDSQDAYPLDATRSAIIRVPAQGFYTVAFEDLYPVQGDADFNDYVAEVRYEQDLD
                     ASGRVARMRGFVRHIARGAGYRHTLRLTLPTTSSSFHSKLFAANGSLELENETALTSF
                     EGIEVMPNSGTTISSANSAAGEVYAPGKSSEFEVIPATPMALASLGNAPYDLHLHVVN
                     TGYDIHFLGRYLDANGHDQYLDPSGFPWAIMIPGNWSWPLERVNMHTAYPKFQEWYQS
                     AGQAQTDWYLTPAAGKVFSK"
     gene            complement(2177..3190)
                     /locus_tag="PLG78_15525"
     CDS             complement(2177..3190)
                     /locus_tag="PLG78_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNE24582.1"
                     /translation="MNLESEIKQVIALCDKNGNLNPSAIGWSRKPLHRCNLSKHYFRK
                     KKWNYWCIYDKEWLISFTISDVDYAGLIFGYYLEFSTGKFAEFTKIVPFSSGITMGQQ
                     TDNNARYQSGGLSFDFQAKKHGQMLLASIPDIGGENLNASIDVEIPEDLESMNVVIPW
                     SKRRFQFTSKQIGLAASGTVTIGTRSHTFNKADTFACLDFGRGVWPYNITWNWGVAVS
                     RSEGEIVGANMGAKWTDNSGYTENSILAGGKTYKLPENVLFEYDRKDFMKPWKIRTEQ
                     SSAIDFEFTPFFDRHAKSNLVILASDVHQMIGKYTGEIKIGRKTVAIHDAIGWAEDHI
                     ARW"
     gene            complement(3243..>3511)
                     /locus_tag="PLG78_15530"
     CDS             complement(3243..>3511)
                     /locus_tag="PLG78_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24583.1"
                     /translation="QKGGRNVKPENVEAARKAGASDDEIHDAVLIAAAFCMYNRYVDG
                     LGTRPPANPADYKEMGKRLSTQGYKFPPRFLYWLVRKILSKKYP"
BASE COUNT          796 a          887 c          876 g          952 t
ORIGIN      
        1 ggtccccggc aatgcgttgt ctttcttctt cctggagttc aatgagtcta ccgttcaatt
       61 tttgaatgcg aatctccagt tccttttctc tggagatatc gcgcatgatg attccagtgc
      121 tatctttttc gaatggaaag agtgagtacc caaatgtgga cttcccggcg cgaaactgca
      181 gaccctttct tgttttgtga ctcttgcccg cctcgcgaac gttagctgca aatgcgtccc
      241 gctcttcccc tttgagtttc aaaaccggaa caaggagcct gttcatcaat ttctccgagg
      301 agattcctgc ccactgcgcg aaggagtcat ttgcaaaccg tatgcgaaga tcggggccta
      361 cgcttacaac cggagaaaga ctcgacagca ggcgagaatt gtagtcgtga attctctgga
      421 attcttccag tgcctccatg cgcgctgtct gattgatcac gactccctgt actccgacca
      481 ctctgcctcg gtcaaagaac ggagttagaa agtattgaac acagtaccga tatccggtct
      541 tgattctgaa atctgcttcc gcaatcacag gttctgcgcg cgaaatgcat aagcgaaact
      601 gcgtggcgaa tccggcgtta gccagatgcc tttctgtcaa caggtttggt ttcttcttct
      661 tttcgagaga aaagaggctt cgcagagttg aattggttgc ctcgatgttt ccctggcgat
      721 ccgctacgaa gaatcccagc ggagcttttt caaagagaga ctgatatctg tcttctaact
      781 tcacgcgcca ggctcctccg gacgcgattc agatgaaagc aaaaagccta gcagtgcaaa
      841 agcttacttc gagaaaacct tgccggccgc aggagtgaga taccagtctg tttgggcctg
      901 gccggcggac tgataccatt cctggaactt tggataggcc gtgtgcatgt tcaccctttc
      961 cagcggccag ctccagtttc ctggaatcat gatggcccat ggaaatccag acggatccag
     1021 gtactgatcg tgaccgtttg catccagata tcttccgaga aaatggatat cgtatcctgt
     1081 attgactacg tgaagatgaa gatcgtaagg agcgtttcca agagaagcta gagccattgg
     1141 agtggccggg attacttcaa attcgctcga tttgcccggc gcatatactt cgcccgccgc
     1201 agagttcgcg gagctgatgg tcgttccgct gtttggcatt acctcgattc cttcaaagct
     1261 ggtcagggcc gtttcgtttt ctaattccag gcttccattt gctgcgaaga gtttagaatg
     1321 gaaggaagaa gaagtggttg gtaacgttag gcgcagtgta tgacgatagc ccgcgcctcg
     1381 tgcaatgtgt ctgacaaatc cccgcattct agcgactcgt ccgcttgcgt ccagatcctg
     1441 ttcgtagcga acttctgcca catagtcgtt gaaatccgca tcaccctgga caggatacag
     1501 gtcttcaaat gcgactgtgt agaatccctg tgcggggaca cgaatgatcg cagatctggt
     1561 tgcatccaga gggtaggcat cctgagagtc agggactccg tcactgtccg catccgcgac
     1621 tagagtcatg ttcacttctc cagtcacaaa aaggaatctg cgaatctctt gcacgtctgt
     1681 gatgttgatg gtccaggtat gcacgtccgt tccgatggtc acgcgaagag tgaccagttt
     1741 cgtggtctgg tttactgtga acgttccttc cacgcttccc tgagcgttgg acgttgcctg
     1801 aaagagaagt tcttgcgcgc ttgagctgtc gaggatttga accagagtgc ccgacccttg
     1861 aggcaccggg tcccgcactg tgatgaagac tggaatggtt cgcgttgtat cgaacaagaa
     1921 gtcaggcgaa ccggtgacat ctgatacatc tacggtaacg ctcgtctctc ccgacggagg
     1981 cgtttgaccg cctgcggcga ggctgaggag ccatacgtaa tttggatcct ctcgtgtaca
     2041 cgaggagaat gccagcatgc aaagtagaag gatgcgtttc atggtcccaa cccacgcata
     2101 gtagaacaca tacgacgaac gaacgcaata tggccaaagg cctggttttc tattcttccc
     2161 gggctatttc acagtgtcac catctggcga tatgatcttc cgcccagccg attgcatcat
     2221 gaatcgcgac tgtttttctg ccgattttga tttcacctgt gtattttccg atcatctggt
     2281 gaacgtctga ggcgaggatc accaaattgg atttcgcatg tctgtcgaag aatggcgtga
     2341 actcaaaatc gatcgcggat gactgttctg tgcgaatctt ccagggtttc atgaaatcct
     2401 ttctgtcata ttcaaataaa acgttctcag ggagcttgta tgtctttcca cctgcaagga
     2461 tagagttctc cgtataaccg ctgttatccg tccatttggc tcccatgttt gcacccacga
     2521 tctctccctc tgatctggag acagccacgc cccagttcca cgtgatgttg tatggccaca
     2581 caccgcggcc aaagtccagg caggcaaagg tgtccgcttt gttgaacgtg tgcgatctgg
     2641 tccctatggt cactgtcccg ctcgccgcga ggccaatttg ttttgaagtg aattgaaagc
     2701 gtcttttgct ccagggaatg actacgttca tgctttctag atcttctgga atctccacat
     2761 caatggaggc gttcaaattt tctccgccga tgtctggtat ggatgcgagt agcatttgac
     2821 cgtgcttttt tgcctggaag tcgaaagaca atccgcctga ttgatagcgt gcattgttgt
     2881 ctgtctgctg gcccattgtg attccgctcg aaaacggaac aatcttggtg aactctgcga
     2941 attttcccgt agagaattca agatagtaac caaagatcag gcctgcgtag tccacatctg
     3001 aaatagtaaa agaaatcaac cattctttgt cataaataca ccagtagttc cacttcttct
     3061 tgcgaaagta gtgtttgctc aggttgcaac gatgcaacgg tttgcgtgac cagccaatag
     3121 cggaaggatt gaggtttccg ttcttatcgc acagagcgat gacctgcttg atttctgatt
     3181 ccagattcat acgtcagccg ggatcattcc aggctggcag taaaacgaaa aatgatttta
     3241 gctcagggat actttttgct aagaatcttt ctgacgagcc agtagagaaa tcgtggagga
     3301 aatttgtatc cctgagtgga cagacgtttt cccatttcct tgtagtcggc gggattggcc
     3361 ggtggcctgg ttcccagtcc gtcgacgtat ctattgtaca tacagaacgc agcggcgatc
     3421 agaactgcat cgtgaatttc atcatcgctt gcgcctgcct ttcgcgcggc ctccacattc
     3481 tctggtttca cgtttcttcc gcccttctgt g
//
LOCUS       DAOQJL010000425         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17124_length_3498_cov_4.227999, whole genome shotgun sequence.
ACCESSION   DAOQJL010000425 DAOQJL010000000
VERSION     DAOQJL010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17124_length_3498_cov_4.227999"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLG78_15535"
     CDS             <1..498
                     /locus_tag="PLG78_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE24584.1"
                     /translation="IDEIDRIASQAEFNKMKMLTGAFARLNPTASMWFHIGPNMHQRE
                     RVFINTMTTQALGLRNPTVLTFISISTPDKANSVIGLADEALRKISKQRADLGAYQNR
                     LEHAARGLMNAYENTQAAESRIRDTDMAEQMTSYTRYQILVQAATAMVAQSNMKSQTV
                     LQLLK"
     gene            complement(549..1493)
                     /locus_tag="PLG78_15540"
     CDS             complement(549..1493)
                     /locus_tag="PLG78_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE24585.1"
                     /translation="MWFGAWGIGGWYWGPPRDQESTSAIEAALQSGIRAFDTAPVYGF
                     GHSEELIGQALQGRRHEAFIATKCSLVWDRPGDPFFTTVHGERSVDVRRNLSRSSILQ
                     ECEESLLRLRTDYIDLYQIHWPLSPLDEAAQALAELKGQGKIRFAGLCNTNVEQIIEW
                     KKLTGSHPDFVQERYSLNQRKIDAGLKPYTLKHSIPILAYSPLAQGLLTGAIRSDRVF
                     GEGDRRGGSTIMDPEYISRIEPALDQARAIASSYNVNLSQLAIAWVLSVPGIGAAITG
                     IRDSLHAKENLSALSIAIKREDSIRLKAIFDPFVNNGK"
     gene            complement(1538..>3498)
                     /locus_tag="PLG78_15545"
     CDS             complement(1538..>3498)
                     /locus_tag="PLG78_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNE24586.1"
                     /translation="IVFGNMGEDSSVGMVYTRDIVTGEDKLSGNFMMNAYTMERTGTP
                     IQDLPAAYLDDLRKICKAVERKFREIREIKFIVEKKKLWLINQTVVDGKSTQSHLRTL
                     LDLMKENVVEESWVVQQIPPGQLASLLQHIVEPASIASLPSASGGMTGSPGAAVGKVY
                     FSADKVMEAHREAMLKGEDTRVILCVESSFAEDVKAIEVGQAVIAVEGGYSSHAPVVA
                     RSLGKVSILNQSIKIGENYFELNGMRINEGDYVTLDCPVYKKPTIYAGKAKLINPDIN
                     TNGLVEFISVMKKFISDDFVVRANADLGRDAKVARVMGAYGIGLCRTEHMFFQDDRIQ
                     RFREMILAKDYEERVKALNELRPMQKNDFYDLFKEMAPHHVTIRLLDAPLHEFLPRND
                     DAFADYTEHLRKKGVTPDEKALRERIERLHEFNPMLGHRGCRVAITYPEIYQMQVRGI
                     FEAACQLQKEGVTILPEIMIPIVMNPNELTFIKNGKKIEGKVITGITDTARDVFKEYG
                     VELEYKVGTMVELPAAAIISDELARYAEFFSFGTNDLTQTTFGLSRDDVNSFFPAYTE
                     FDILPNNPFQVLAPPVKELVKMSATRGKLTRPDLKLGLCGEHGADPANIAFCKEAGLH
                     YVSCSPYSVPIALLAVAQMNLKAGPGGK"
BASE COUNT          839 a          932 c          861 g          866 t
ORIGIN      
        1 atcgacgaaa tcgacagaat tgcatcccag gcagaattca acaaaatgaa gatgctgaca
       61 ggcgcgttcg ctcgcttgaa tcctacagct tctatgtggt ttcacattgg accaaacatg
      121 caccagcgcg agagagtgtt catcaacacg atgaccacac aggctctggg ccttcgcaac
      181 ccgaccgtac tgacgttcat ttcgatcagt acaccggaca aagcgaacag cgtgattgga
      241 cttgctgatg aagcacttcg caaaatctcc aagcaaagag cggatcttgg tgcttaccag
      301 aaccgtctcg agcatgcggc acgtggcctc atgaacgctt acgagaacac gcaagcagct
      361 gaatcccgca tccgtgatac ggatatggcg gagcaaatga cgagctatac tcgttatcag
      421 attcttgttc aggcagcaac agccatggtt gcacagtcga acatgaagag ccagaccgtt
      481 cttcagcttc tgaagtaaca gtcacttccc gatcctgatg tagactgccg gccttcgggc
      541 cggctattct attttccatt gtttacgaat ggatcgaaga tggccttcag acgaatgctg
      601 tcttccctct tgatcgcgat agacagcgcg cttagatttt ccttcgcatg aagcgaatct
      661 cgaatgcctg taatggccgc tccaatgcct ggaacagaaa gcacccaggc aatcgcaagc
      721 tgcgacaagt ttacgttata cgacgaagca atggcacgag cctgatcgag agctggttct
      781 attctcgata tgtattcagg atccatgata gtggacccgc ctctccgatc cccttccccg
      841 aagacccggt ctgatcgtat ggctccagtc agcaaaccct gggccagtgg actgtaggct
      901 agaatcggta tggagtgttt tagcgtgtag ggttttagac ccgcgtcgat ctttctctga
      961 ttcagactgt atctttcttg aacaaaatca ggatgagacc cggtaagctt tttccactcg
     1021 atgatctgct cgacattggt attgcaaaga cccgcaaatc gaatcttgcc ctgtcccttg
     1081 agctccgcca atgcctgagc tgcttcatcc agaggcgaca ggggccagtg gatttgatag
     1141 aggtcaatgt agtccgtgcg cagccgcaaa agactctctt cgcactcctg caaaatagaa
     1201 gagcgactta gattgcgcct aacgtcgaca gaacgctcgc cgtgaaccgt agtgaagaat
     1261 ggatctcctg gcctatccca gaccagagaa cacttggttg cgatgaatgc ttcgtgtcgc
     1321 ctgccctgca gtgcctgacc gattagctct tcagaatgac cgaatccgta tacaggggct
     1381 gtgtcaaaag cgcggatgcc agactgaagg gcggcttcga tggcagatgt ggattcctgg
     1441 tcgcgcggtg gaccccagta ccaaccaccg atgccccacg ctccgaacca cacccgggga
     1501 agatccccgg ggagaagaca agacggaacc gaaggaatta cttccctccg ggaccagcct
     1561 tgaggttcat ttgcgccaca gcaaggagcg caatggggac gctatacgga gaacaagata
     1621 catagtgcag accggcttct ttgcaaaatg caatgttggc aggatctgct ccgtgttccc
     1681 cgcagaggcc cagcttcaag tctggtcttg tgagtttgcc acgagtggca ctcatcttca
     1741 ccagttcttt gactggaggc gcaagaacct ggaacgggtt gttcggaaga atgtcgaatt
     1801 ctgtgtatgc cgggaagaac gaattcacgt catctcgtga cagaccaaac gtagtctgtg
     1861 tgaggtcatt ggttccaaag ctgaagaatt cagcgtagcg cgccagctca tcggagatga
     1921 ttgccgccgc cggaagctcc accatggtcc ctactttgta ctccagttcc acgccgtact
     1981 ctttgaatac gtcgcgagcc gtgtctgtga tacccgtgat cacttttcct tcgatcttct
     2041 taccgttctt gatgaacgta agttcgtttg gattcatcac aattggaatc atgatctcag
     2101 gaagaattgt aacgccttct ttctgcagct ggcatgctgc ttcaaagatt ccgcgcactt
     2161 gcatctggta gatttccgga tacgtaatgg ccactctgca accgcgatgc ccgagcatcg
     2221 ggttgaattc atgaagcctt tcaatacgct cgcgaagagc tttttcatcc ggagtcacac
     2281 ccttcttacg aaggtgctct gtgtagtctg cgaacgcatc gtcgtttcgc ggaaggaact
     2341 catgcagagg cgcatcgaga agacgaatgg tcacatggtg aggcgccatt tccttgaaca
     2401 gatcgtagaa atcgttcttc tgcattgggc gcagctcatt gagagctttc acgcgttctt
     2461 cgtaatcctt tgcaaggatc atctcgcgga atctctggat acgatcatcc tggaagaaca
     2521 tatgctccgt acggcagagg ccaattccgt aagcacccat cacgcgcgct actttagcat
     2581 cgcggcccag gtccgcattt gcgcgaacca cgaagtcatc actaatgaat ttcttcatta
     2641 cagaaatgaa ctcaacgaga ccgtttgtgt tgatgtctgg attgatgagc ttagcttttc
     2701 ccgcatagat ggtaggcttt ttgtaaaccg ggcagtcgag tgttacgtag tcgccttcat
     2761 tgatgcgcat tccattgagc tcgaagtagt tctcaccaat cttgatgctc tggttgagaa
     2821 tgctcacctt gccgagtgat cttgccacca caggagcgtg agaagaatac ccaccttcca
     2881 cagcgatgac tgcctgacca acctcaattg ctttcacgtc ctctgcaaaa gaagactcca
     2941 cgcataagat cactcgagtg tcttcgccct tgagcattgc ttcgcggtgc gcttccatca
     3001 ctttatcagc ggagaaatag acctttccta cggccgcgcc aggagaacca gtcattccac
     3061 ccgaagcact gggaagactt gcaattgaag ctggttctac aatgtgctgg agcagacttg
     3121 ccagctggcc tggaggaatc tgctgaacca cccacgattc ttccacaaca ttctctttca
     3181 tgagatccag aagagttctc agatgggatt gagtggattt gccatctaca accgtctggt
     3241 tgatcagcca tagtttcttc ttttctacaa tgaatttgat ttcacgaatc tcgcggaact
     3301 tgcgctctac cgctttgcag attttgcgaa ggtcatcgag ataggctgca ggcagatcct
     3361 gaatcggtgt tccagtgcgc tccatcgtgt atgcgttcat catgaagtta ccggacagct
     3421 tgtcttctcc agtaacgatg tcgcgtgtat acaccatacc aacgcttgag tcttcgccca
     3481 tgttgccgaa cacgattc
//
LOCUS       DAOQJL010000426         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17185_length_3490_cov_4.719360, whole genome shotgun sequence.
ACCESSION   DAOQJL010000426 DAOQJL010000000
VERSION     DAOQJL010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17185_length_3490_cov_4.719360"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1368
                     /locus_tag="PLG78_15550"
     CDS             136..1368
                     /locus_tag="PLG78_15550"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24587.1"
                     /translation="MRISKIWSRLSQVGVSFEPDPEERKRIILVNKTSITVFLVSIVI
                     PVVPIQFNLDRVALAQFTASFAYLLVPVLNSFHRFFQAKLALMIFGVLHIGVFAHVLG
                     EVSATHFFIFAPALGAYVAFSPRDWRIRTFCTLFSLAVLILLLCDFAGKPLEFKDFWA
                     RIYYYFSLVLALVLCLFLMHHLYSTSMEVEGKLAEERKRSDELLLNILPAKIAEELKE
                     SGAAEPVHFDSVTIVFTDFQGFTRSAESMPARELVRELDEYFRAFDEICERFGLERLK
                     TIGDSFMCAAGVPEISSDHAIVALRAALAMRDYSLSRTKGPRWGVRIGVHSGPVVAGV
                     IGRKKFAYDLWGDTVNTASRMESSGEPGAVNCSEATKSLAGEVFTFESRGKIEAKGKA
                     PMEMFFCLGEGKNFHSLS"
     gene            1401..1517
                     /gene="rrf"
                     /locus_tag="PLG78_15555"
     rRNA            1401..1517
                     /gene="rrf"
                     /locus_tag="PLG78_15555"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1565..2677
                     /locus_tag="PLG78_15560"
     CDS             1565..2677
                     /locus_tag="PLG78_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE24588.1"
                     /translation="MFIPAFWLSFSGPTFMMTLLAFGAGLSFLLAISRAIRRTPESYA
                     AAALFSCASIVLLHLYLELTARLSAFPHFVYWNVPALFCTGPLTHFYLLMLMRSPGER
                     KPGWIHFLPAIVSFFILIPFFIQSGETKTAFVTVPGFDASNPYQFIGMALISLGVLVN
                     LFYLLFLFARIFRLRSVPGNWDRSALSPFLIVLGATTVAILAMTLAQFFFKEIMHVAA
                     ASLGALIVILFLFGETSVRDDFAREIKRAKYAQSRIAGLDVNAVLARLDQIMREERAY
                     LDEDVSLARLADDLQIRSQQLSEILNARIGLGFRDYINGFRLEEAARLLIEEPRRSIL
                     SILFASGFNSKSSFHKLFQARFHCSPAEYRQKQTEV"
     gene            2742..>3490
                     /locus_tag="PLG78_15565"
     CDS             2742..>3490
                     /locus_tag="PLG78_15565"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24589.1"
                     /translation="MSKIKQHILTGTALAAAFLLQLFSSGNWMSASAPWVGLPIWLLL
                     FRYGNRAIVSVAAVVFLGASLVAWRGMIDDLFLRFAVIGGIGSLTLLLFLIDRILVRR
                     LVESRGVCVASFVFPTALTAMELILTRLSPYGSWSILGYTQSSNLPLIQMAAVTGVYG
                     VSFLIGWVSSVLCFLVRYAFAPEESSVSLGFHRVRLLVVSCFVLSGSIFLMGTVRMTL
                     SQETPSVRVAGITVDMSHSRGDVLHSVFDGS"
BASE COUNT          696 a          818 c          911 g         1065 t
ORIGIN      
        1 ggtacagcag gccatcaaaa tgattcgcag gcctctgact gagacttctg tcttcgatcg
       61 cgtttgcagt cgagccacag gtttgaaaga agcctatcgc atgaaagagt gagttttact
      121 ttcaaagaaa ctctcatgag gatcagcaaa atctggagca gactgtctca ggtcggagtc
      181 tcgtttgagc cagaccccga agaaagaaag agaatcattc ttgtaaataa gaccagtatc
      241 acagtatttc tggtgtctat cgtgattcca gttgttccca ttcagttcaa cctggatcgc
      301 gttgcgctgg cacaatttac tgcttctttc gcttacttgc tggttccagt tctgaattcc
      361 tttcacagat tcttccaggc aaagcttgct ttgatgatct ttggagtgct gcacattggt
      421 gtcttcgcgc acgtgctggg ggaagtatcc gccacccatt tctttatctt cgcgcccgct
      481 ctgggagcgt acgttgcctt ttctccgcgc gattggcgca ttcgcacgtt ctgcactttg
      541 ttttctcttg cggttttaat tttgctgctt tgcgattttg caggcaagcc gcttgagttt
      601 aaggatttct gggccaggat ctattactat ttcagcctgg tgcttgccct ggttctttgt
      661 ctttttttga tgcatcatct gtactccacg agtatggagg tggaaggcaa actcgcagag
      721 gaaagaaaga gatccgacga actattgctc aacatccttc ctgcgaagat cgcagaagaa
      781 cttaaagaat ccggagcggc agagccagtt cattttgatt ccgtgaccat tgtatttact
      841 gattttcagg gtttcacccg atctgcggag agcatgcctg ctcgcgaact cgtgcgcgaa
      901 ctagatgagt actttcgcgc gtttgatgaa atatgtgagc gctttggtct cgaaagactc
      961 aagacgatcg gagacagctt tatgtgcgcc gcaggtgtcc ctgaaatttc gagcgatcat
     1021 gcaatcgtcg ctcttcgcgc agctctcgcc atgcgcgact attctctttc gcgcacaaaa
     1081 ggcccacgct ggggagttcg aattggagtt cattctggtc cagtggttgc gggagtgatt
     1141 ggtagaaaga agttcgccta cgatctctgg ggagatactg taaacacagc aagtagaatg
     1201 gagtcttctg gagaaccagg cgcagtaaat tgttcggaag ctacaaagtc gctggcggga
     1261 gaagtgttca cgtttgaatc gcgcggaaag attgaagcga aaggcaaggc acccatggag
     1321 atgttttttt gtctgggaga agggaaaaat tttcacagtt tgagttgact cgcagggggt
     1381 tggcaattat gttggtattg tctggtgcca atagagaggg tggtacacct gttcccattc
     1441 cgaacacaga agttaagccc ctcatcgcca atggtactat gaggttcgcc tcatgggaga
     1501 gtaggacggt gccgacattg caatcatcta gatcattcca caaaggctcc tgcgggagcc
     1561 tttcttgttt attcccgctt tctggctctc cttcagtgga cccactttca tgatgacatt
     1621 gcttgccttt ggtgccgggc tttcttttct cctggcaatt tctcgcgcca tccgccgcac
     1681 tccggagtct tacgcggcgg ctgcattgtt ttcgtgcgca agcattgtgt tactgcatct
     1741 ctatctggag ttgaccgcgc gactttctgc ctttccccat ttcgtgtact ggaatgtgcc
     1801 tgctcttttt tgcaccgggc ctctgactca tttttatctt ttgatgttga tgcgatctcc
     1861 tggagaacgc aagcccggct ggattcattt tttacccgcc atcgtttcgt tttttatttt
     1921 gattcctttc tttattcaga gtggagagac aaaaacagcg tttgtgactg tgcctggatt
     1981 tgatgcaagc aatccgtacc agttcattgg gatggctttg attagtctgg gtgtgcttgt
     2041 gaatttgttt tatcttttgt ttttgtttgc gcgaatcttc aggctcaggt ccgttccagg
     2101 gaactgggac agatccgctc tttctccgtt cttgattgtg ctgggagcta ccaccgttgc
     2161 gattcttgcc atgactctgg cgcagttctt cttcaaggag atcatgcacg tcgcggcggc
     2221 gagtctgggc gcattgattg taattctttt tttgtttggt gaaaccagtg tacgcgatga
     2281 ttttgcccgc gaaatcaagc gtgcaaaata tgcgcaaagt agaatcgcgg ggctggatgt
     2341 gaatgcggtt ctggccaggc tggatcaaat catgcgcgag gaaagggcgt atctggacga
     2401 agatgtgtct ctggcgcgcc tcgcagacga tctgcagatt cgttcgcagc aactctcaga
     2461 gattctgaat gcaaggatag gtcttggctt tcgcgactac atcaatggat tcaggctgga
     2521 ggaggccgcg agactcttga ttgaggagcc gcggcggtcc attctatcga ttctatttgc
     2581 gtccggtttc aattcaaaat cgtcctttca caagctcttt caggcacgct tccattgttc
     2641 gcccgcggaa tatcgccaga aacaaactga ggtgtaatga tttttgagtc cgtccgtatc
     2701 ccgacggacg actttgctct tgtttcgtgt tccaatggag tatgagcaaa ataaaacaac
     2761 acatactgac ggggactgct cttgcggcag catttctact ccaactattc agcagcggca
     2821 actggatgtc cgcaagcgct ccgtgggtgg gccttcccat ctggcttctt ctttttcgat
     2881 atggaaaccg cgcgatcgtg tccgtggccg cagtcgtttt tctaggggct tctcttgtgg
     2941 cgtggcgcgg aatgatagac gatctatttc tgaggttcgc cgtgataggc ggcattggtt
     3001 ctttgacttt gcttctattt ttgatcgatc ggattttagt tcgcaggctg gttgagtctc
     3061 gcggagtctg tgtcgcgagc tttgtatttc caaccgcttt gactgcgatg gaattgatcc
     3121 tcactcgttt gagtccgtac ggaagctgga gcattctggg gtacacacag agttcgaatc
     3181 ttcctttgat tcaaatggca gcagtcacgg gagtttacgg cgtttctttt ttgatcggct
     3241 gggtttcttc tgttctttgt ttccttgtta gatacgcgtt cgcgccagaa gaatcgtccg
     3301 tctcgctcgg gttccatcgc gttcgccttc ttgttgtttc ttgtttcgtt ttgtctggtt
     3361 ccattttcct catgggcact gttcgcatga ctctgtcgca ggagactccg tcggttcgcg
     3421 tggccggcat cacagtcgac atgagccatt cgcgaggaga tgttttgcat tctgtgtttg
     3481 atggatccat
//
LOCUS       DAOQJL010000427         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17243_length_3483_cov_4.412485, whole genome shotgun sequence.
ACCESSION   DAOQJL010000427 DAOQJL010000000
VERSION     DAOQJL010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17243_length_3483_cov_4.412485"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLG78_15570"
     CDS             complement(<1..326)
                     /locus_tag="PLG78_15570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE24590.1"
                     /translation="MLRHFSIAGARARENLPADEASMTDTKAKWVALIILCLGDLMIV
                     LDTTIVNVALPSIQRSLGFSEANLVWVVNAYMLTFGGFLLLGGRLGDLFGHRRLFLVG
                     ISIFTIA"
     gene            511..2034
                     /locus_tag="PLG78_15575"
     CDS             511..2034
                     /locus_tag="PLG78_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24591.1"
                     /translation="METPGGANRVKTFAAAGLIAASLLVILCNLRFKQLYSIDSDTVD
                     AFLMLEAAGWHAHTPFHPGLLLGFSPYVLLKGMAEVSRFDAMRLNFAFYGCLAVLGCF
                     AFAFVVTRDSLRAAACTLLFSLTPAVQFVERFLDDNFPSYGFLFLVLTGLLSPGPPTT
                     RLTVRVAISLLFFPPLLLFQPGFLPCLIVSGLGMIYMTFLFDWTWKDRLRTGALTALA
                     FTLSACFTFWALGAWLGVSVSALLHGMGSAIVDTSSNVASQTLPFVDRRGSFAFGLKH
                     SIFAGSILHSLDLNDLSYVLIGYDRMKGDFTALALGIAITIFVFFRGWKTKSNRDKFN
                     AVTGASGLVVLCAIPYAAANLVSQQERFDLLTVLLPFLLARVSFQGQSPWSFSNVWIL
                     RLLAAIFLAGSITLLTPSSPESSWQRMKRILPEAPAASYLFAEGELRNHEDSVSLAML
                     GALPLSVIENPQGPEGVLAILRWMRPRYLISIDQARTLDLHNTFVSAEARNRLASRP"
     gene            complement(2043..2978)
                     /locus_tag="PLG78_15580"
     CDS             complement(2043..2978)
                     /locus_tag="PLG78_15580"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24592.1"
                     /translation="MKAFYVCRTTNLDTYLVNADIVTPAQSQAIGASTSWMRTDLDGK
                     YLYIQSSGNNVDWYSIDRDTGLIARVGTFAIALAGNYEPSPYYKNIMVASLGASKMRT
                     FSIGSDGSLTTLFINQDPGSTSPGKFVFHPTLPVVYTLETSTVRIQDLAANGALTFRT
                     TAAATATTTNIAIDPNGRYLAVANNAAPGIQLYTIDSTGGLSAPSSAAFTNISILAFD
                     STGSFLMAGNTALPGVTTVNIPAMQSVQTFNTGTLPTTVIADRGGKYVLVGNPAPTLF
                     SLYQFTQTGGLPSVKYEFVPTGNVTGVGMYSPYGF"
     gene            complement(3278..>3483)
                     /locus_tag="PLG78_15585"
     CDS             complement(3278..>3483)
                     /locus_tag="PLG78_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24593.1"
                     /translation="VGDPFKDTSSVAMNPIIKFTTLFGLLAVELAIETPDTVRLALAG
                     VFFIVSTVFVWRSFYAMRIEQKG"
BASE COUNT          800 a          883 c          898 g          902 t
ORIGIN      
        1 gcaatcgtaa agatagaaat tccgacgaga aacaaacgtc tgtgtccgaa aagatcgcca
       61 agtcgtccgc ccagaagtag aaatcctccg aacgtcaaca tgtaagcatt cactacccag
      121 acaagatttg cttctgaaaa acccaggctt ctttgtatag aaggaagtgc tacatttaca
      181 atcgttgtgt ccaggacgat catcagatct cccagacata gaatgatcag ggcgacccat
      241 tttgcttttg tgtctgtcat gctcgcttca tccgcaggca ggttttcgcg agcgcgagca
      301 cccgcaatcg aaaaatggcg gagcaacttt agacaaatcg tcgcacgaat gaacgaagca
      361 cctaacctcc tatgccgaag cgtcttagaa tcgcataacg gtaagcgatc cgtccacgcg
      421 cagatttttc cgagtcccgg aaccgtgtgc aaagagtagc ccgggaagga atggacttga
      481 ctgattcctt tttcttttga ctttaaatgt atggagacac ccggcggggc aaatcgagtg
      541 aagaccttcg ctgccgcagg attgatagca gcgagtcttc tggtcattct gtgcaattta
      601 agatttaagc aactgtactc gatcgactca gatactgtag acgcatttct gatgcttgaa
      661 gctgcgggct ggcatgcgca tacacccttt catcctggat tgctgctcgg attttcgcct
      721 tacgtgctgc taaaggggat ggccgaggtt tcccggttcg atgcgatgcg tttgaatttt
      781 gctttctatg gatgcctcgc tgttctcggg tgtttcgcat ttgcattcgt agtgactcgc
      841 gattctttgc gcgccgccgc ctgcaccctg ttgttttctc tcacgccggc ggtccaattt
      901 gtagaacgtt ttctcgacga caattttccg tcatacggat tcttgttttt ggttttaact
      961 ggtttacttt ctcctggacc gcccaccacg cggttaacag tccgagttgc gatctccctg
     1021 ttgttttttc ctccgctgct gttgtttcaa cctgggtttc taccttgctt gattgtgagc
     1081 ggccttggta tgatctacat gacgtttttg tttgattgga cctggaagga tcgcttgcga
     1141 acgggtgcgc tcactgctct tgcttttact ctctctgcat gcttcacatt ctgggctctg
     1201 ggagcctggc ttggcgtctc ggttagcgcg ttgcttcatg ggatgggttc tgcgattgta
     1261 gatacgtcaa gcaatgtagc ttcgcagacg cttccttttg tggatcggcg tgggtctttt
     1321 gcctttggct tgaagcacag catctttgcc ggttcaatct tacattctct cgacttaaac
     1381 gatctctcct atgtgttgat tggttacgat cgcatgaagg gcgatttcac tgcactggcg
     1441 ctggggattg caatcaccat cttcgtcttc tttagaggat ggaagaccaa aagcaataga
     1501 gataagttca atgcagtgac gggtgcgagc ggccttgtgg tactctgtgc gattccgtac
     1561 gccgccgcca atcttgtgag ccagcaggaa aggtttgacc tgctcacagt tctcttgccg
     1621 tttctgctcg cacgcgtgtc tttccagggg cagtctccgt ggagtttctc gaacgtttgg
     1681 atcttaagac tgcttgcagc tatattcctc gccgggtcca ttacattgct tacaccttca
     1741 agccctgagt cgtcgtggca acggatgaaa cgaattttgc cggaggcacc ggcagcttct
     1801 tacttgtttg cggagggaga gttgcgcaac cacgaggatt cagtatccct ggcgatgctg
     1861 ggagcactgc cactctccgt gatcgagaat ccacaagggc ctgagggcgt tctggcaatc
     1921 ctcagatgga tgaggccgcg ctacttgatt tccatagacc aggcaagaac gcttgatttg
     1981 cacaatacct tcgtaagcgc cgaggcacga aatcgcctgg cttcgcgtcc gtagcgcgcc
     2041 agctagaatc cgtagggact gtacattccg accccggtga cgttgcctgt tggaacgaac
     2101 tcatatttca cagacggcaa tccgccagtt tgcgtaaact ggtatagaga gaacaaagtc
     2161 ggcgcgggat ttccaacgag tacatacttt ccgccgcggt ctgcgatgac ggtggtgggc
     2221 agagtgcctg tattgaaagt ctgtacagat tgcatggcag gaatgttcac cgtggttaca
     2281 cccggcagtg cggtgttccc tgccataaga aagctccccg tcgaatcaaa cgcaagtatg
     2341 ctgatgtttg taaatgcggc gctcgaagga gcactcaggc ctcctgtaga atcaattgta
     2401 tagagctgaa ttccgggtgc ggcgttgttg gcaacggcga gatacctccc attaggatcg
     2461 atggcgatgt tcgtggtagt cgcagttgca gccgcagttg tgcgaaaagt aagcgcaccg
     2521 tttgccgcca ggtcttgaat tcgaactgtg cttgtctcaa gagtgtacac aacgggaagc
     2581 gttggatgaa acacgaattt tccaggcgag gtgctgcccg ggtcctggtt gatgaaaaga
     2641 gtcgtcagac ttccatctga gcctatgctg aacgtacgca tcttggatgc acccagagac
     2701 gccaccatga tattcttgta gtaagggctg ggttcataat taccggccag agcaattgca
     2761 aacgttccca cgcgcgcgat caagcctgta tctcgatcaa tggagtacca gtctacgttg
     2821 tttccagaag attgaatgta caaatacttc ccgtctagat ctgttcgcat ccagcttgtt
     2881 gaagctccaa tcgcttgcga ctgagcgggc gtaacaatat cagcgttcac aagatatgtg
     2941 tccagattcg tagtgcgaca gacgtaaaag gccttcatcc gataagaccg ctgggcagaa
     3001 agtaacaacg cccccacgct gcaggaagga tcccctttcg cgcaatcagt agacacacgg
     3061 cacgcactga cgaaagccag gaccagaagc acggcaaaac gcatggcaac aagatatcgc
     3121 gtggccgggt gcctgtctct ctttttagct agccgcaaaa ccgccattat gcgatcctca
     3181 ttcacccatt tcgggttgcc gggccaaaaa aaaacccggg gaaaccccgg gttttgcaga
     3241 cctccccaaa aagaaggtcc tattcagaat ccgccattta gcctttctgt tcgattcgca
     3301 tcgcgtagaa agatctccac acaaatacag ttgaaacaat aaagaacacg cctgcgagag
     3361 ccagacgaac tgtgtccgga gtttcgattg cgagttcaac agcaagcaga ccaaacagag
     3421 tggtgaactt gatgatcggg ttcattgcaa cagaagatgt gtctttgaaa gggtcaccta
     3481 cag
//
LOCUS       DAOQJL010000428         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17391_length_3465_cov_4.694721, whole genome shotgun sequence.
ACCESSION   DAOQJL010000428 DAOQJL010000000
VERSION     DAOQJL010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17391_length_3465_cov_4.694721"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PLG78_15590"
     CDS             complement(<1..1907)
                     /locus_tag="PLG78_15590"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24594.1"
                     /translation="MARYLLCLLLVLTAACNRGDLIPLGGTWDFKKGFDQKDLTDPSR
                     SGWDTVKLPASITETKNLQGYAGWITLRKELPPESADILSRGAAAINMGWTSDVSYYY
                     INGQLIGQVGSAQPYETGLFRRFIKEIPQVPAKEKNYLVIAVYGTGEYPLQFDTDAYF
                     GPGESVFNEYFQNEVVSFFLLGIYFVVGAYHLLLFSRRPQDKHNLLFGIFCTLLTLYW
                     LNRTGSRDPLFGSHVIARLRIEYISLFFIPPSLMFFLSQFFYRRFSRVGLAVFAVCLV
                     LAVITLFAPYTVAKNCLTVWQYSSIVAIGYAIFYILREAFKKNQDARWLALGVIVLMI
                     AALHDIAAARHWINSTHIARYAFLIFVLAIAAILANRFMRVHTEVEQLNEKLEDKVRV
                     RTQQLTESLDEVQKLKIQQDGDYFLTSLLIQPLTGNFVKSQNVSVDMLLRQKKQFQFR
                     HWQAEIGGDLCIAQSISLRRRTYTVFLNGDAMGKSIQGAGGALVLGTVFKSMVSRTQL
                     AEAAQNKAPEQWLKECFVELQNVFVSFDGSMMVSAVFGLVDEESGMLYYINAEHPFVV
                     LYREKKAEFLENDMQLRKLGVSGLGGHLEVKTYQLLPNDVVIIGSDGRDDVQIGVDRL
                     GNRIINENEME"
     gene            complement(2006..2779)
                     /locus_tag="PLG78_15595"
     CDS             complement(2006..2779)
                     /locus_tag="PLG78_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE24595.1"
                     /translation="METVLVTGGASGIGRAIAQEYIAKGARVILADKNRALLQSTARS
                     LGCDWVEMDVTSSSSIKKARANVIKKVGGVDVLVNNAGVVFGGRFEDVSVERHSLTYQ
                     VNTLGPVLVTHAFYPDLLSRPGSKIVFVASASGYIGLPRGTTYASSKWSIVGFAESLR
                     AEVEETGRGPSVTLVCPSYVDTGLFAGAKAPLLTPLLHAGDVGRRIVDAAERGRPVLN
                     MPWIVNIIPWIRSVLPRFLYKAILRITGVTRSMDDWHGR"
     gene            complement(2805..>3465)
                     /locus_tag="PLG78_15600"
     CDS             complement(2805..>3465)
                     /locus_tag="PLG78_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24596.1"
                     /translation="VPLTLGDAPPQEPEGILPGDLYREIAETELFQSGRFEVIPYYKF
                     AEERAKIDPEKKDPTSVLKTAQSMGIQLLISGDCTDFEIKQSRSFWKIPLWAIILAAS
                     FAIDDRNLRAVIWEAMIRMLFVVPLNSPFWDEGIGWEDTDLDVLVSLNMRASGPSGSI
                     RFSDESAVKRTESVRNLDLLVSKESRKVRIQKASAGRQIRFATSDLVRKISREADQGL
                     R"
BASE COUNT          912 a          937 c          829 g          787 t
ORIGIN      
        1 aattccattt cgttttcatt gatgattcta tttcccagtc tatctacgcc gatctgcacg
       61 tcatcgcggc cgtcagatcc aatgatcact acgtcattgg gcaagagctg atacgttttc
      121 acttccagat gaccgcccag gcctgaaacg ccgagctttc gcagttgcat gtcattctca
      181 aggaactcgg cttttttctc tctgtataga actacaaacg ggtgctccgc attgatgtaa
      241 tacagcatcc cgctctcttc gtccacgaga ccgaatacag ccgaaaccat catgctacca
      301 tcaaaagaaa cgaaaacatt ctgaagctct acaaagcatt ccttgagcca ttgctcgggg
      361 gctttgttct gcgccgcttc cgcaagctgc gtacgcgaaa ccatagactt aaatacagtt
      421 cccagaacca gagcgccacc tgcaccctga atggattttc ccattgcatc cccgttcaaa
      481 aagacagtat aggtgcgacg cctgaggctg atgctctgcg cgatgcacag atccccgcca
      541 atttccgcct gccagtggcg aaactgaaac tgtttcttct gacgcaaaag catatccaca
      601 cttacattct gacttttcac aaagtttccc gtgagtggtt gaatcagcag ggaagtaagg
      661 aaataatccc cgtcctgttg gatttttagc ttctgcactt catcgagtga ttcggtcaat
      721 tgctgtgtgc gaacccgaac cttgtcttcc agcttttcgt tcagttgctc cacttccgta
      781 tgcacgcgca taaagcgatt ggcaagaatg gcagcaatgg ccagcacaaa gatcaagaac
      841 gcataacgtg cgatgtgagt actattgatc cagtgccttg cagcagcaat gtcatgaaga
      901 gctgcgatca tgagaacgat gacgcccaga gcaagccagc gcgcgtcctg attctttttg
      961 aaagcctcgc gaagaatata gaagatagcg tatccaatcg ccacgataga gctgtactgc
     1021 cagacggtga gacaattctt cgcaaccgta tacggggcaa aaagggtgat gaccgcaaga
     1081 acaagacata ctgcaaaaac cgccagacct acgcgcgaaa aacggcggta gaagaactga
     1141 gaaagaaaga acatcaggct gggcggaatg aagaacaaac ttatatattc gatgcgtaga
     1201 cgagcgatca cgtgagagcc aaacaatgga tcacgagatc ccgttcgatt gagccagtat
     1261 aaagtcagaa gagtacagaa aattccaaac agtaaattgt gcttatcctg gggtctgcgc
     1321 gaaaacaata aaagatgata cgcgcccacg acgaagtaga ttcccaatag aaagaaggaa
     1381 accacttcgt tctgaaagta ttcattgaaa acggactccc ccggtccgaa atatgcgtcc
     1441 gtgtcaaatt gaagcggata ctctccggtc ccgtatacag cgatgaccag gtagttcttc
     1501 tcttttgccg ggacttgcgg aatttccttg atgaagcgtc tgaacaatcc ggtctcgtac
     1561 ggctgcgcgc ttcccacctg gccaatgagc tggccattga tatagtaata cgaaacatca
     1621 gaagtccaac ccatattgat agcggcggcg ccacgggata gaatgtcagc ggattcgggt
     1681 ggaagttctt tgcgaagagt gatccagcct gcataaccct gcagattctt tgtctctgta
     1741 atgcttgcag gaagcttcac tgtgtcccag ccagagcggg acggatccgt tagatccttt
     1801 tggtcaaagc ccttcttaaa atcccaggtt cccccgagag gaatcaggtc ccctcgattg
     1861 caggccgcgg tgaggacaag gagcaggcag agtagataac gcgccacggg taaatcgtcc
     1921 cttcgagcca ggtcaggtca agagattctt agacggctcc gccgggacgc caaatacccc
     1981 aatttcgccc aggatcgctt cccgctcagc gaccgtgcca gtcgtccata cttcgcgtaa
     2041 caccggttat gcgaagtatt gccttataca gaaatcgcgg aagaacagat cttatccagg
     2101 ggatgatatt gacgatccat ggcatgttga gtactggccg acccctctcc gccgcgtcta
     2161 caatacgtcg gcccacgtcc cctgcatgca gcaacggcgt caaaaggggt gccttcgcgc
     2221 ctgcgaagag gccagtatct acgtaagacg ggcatacgag ggtaacagaa ggccccctcc
     2281 ccgtctcctc aacttcagcg cggagagact ccgcgaagcc aacgatagac catttactcg
     2341 acgcgtaggt ggttcctctg ggcagtccaa tgtagcccga tgcgcttgca acaaacacga
     2401 tcttgcttcc tggccgcgaa agcaaatccg gatagaaagc gtgagtcacc agcacagggc
     2461 ccagagtatt gacctgatac gtgagagaat gacgctctac acttacatct tcgaaacgtc
     2521 cgccaaagac cactccagca ttgttgacca gtacatcaac cccaccgact tttttgatca
     2581 cattcgcgcg ggctttcttt atggatgagg aactggtaac atccatctca acccagtcac
     2641 atcccaggct gcgtgcggtg gattgaagga gagcgcgatt cttgtccgca agaatgactc
     2701 gagcgccctt tgcgatgtac tcctgcgcga tggcacgccc gatcccgctt gcgccccccg
     2761 taaccagaac tgtctccatg aacaaaaaat cagaatgatg gatttcagcg caagccttga
     2821 tccgcttcgc gggagatttt gcgaaccaga tcagatgttg cgaatcgaat ttgtctgcct
     2881 gcagatgctt tctgaattcg aacctttcgc gattctttcg agacaagaag atccagattc
     2941 cgcacgcttt ctgtccgctt cacggcagac tcatccgaaa atcgaatgct gccagaaggt
     3001 ccgctcgcgc gcatgtttag agatacaagg acgtccagat ccgtgtcttc ccagccaatc
     3061 ccctcatccc agaaaggaga attgagcgga acgacgaaga gcatgcgaat cattgcttcc
     3121 cagatcactg cacgcaaatt tcgatcatca atggcaaagc tggcagccag tatgatcgcc
     3181 cagagaggaa tcttccaaaa actcctggat tgcttgattt caaaatcagt gcaatcaccg
     3241 gaaatcagaa gctgaatgcc catcgactgc gcagtcttca agacggacgt aggatccttc
     3301 ttttccggat caatcttcgc acgttcttct gcgaatttgt agtaagggat cacttcgaat
     3361 cttccagatt gaaagagttc tgtttccgct atttcacggt aaagatctcc tgggagaatg
     3421 ccctccggtt cttggggagg tgcgtccccc agggtgagcg gaaca
//
LOCUS       DAOQJL010000429         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17486_length_3454_cov_3.907326, whole genome shotgun sequence.
ACCESSION   DAOQJL010000429 DAOQJL010000000
VERSION     DAOQJL010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17486_length_3454_cov_3.907326"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLG78_15605"
     CDS             complement(<1..258)
                     /locus_tag="PLG78_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24597.1"
                     /translation="MSRLKVQLAALGGMVVITFLLHVFLFGEAGYFTRQKLQDRLHAI
                     RGQIEELETENRTLEEEYAKIEETPAARSSAPGATILTFETN"
     gene            complement(294..1583)
                     /gene="eno"
                     /locus_tag="PLG78_15610"
     CDS             complement(294..1583)
                     /gene="eno"
                     /locus_tag="PLG78_15610"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE24598.1"
                     /translation="MHTIHAREILDSRGNPTVEVDVILADGTLGRAAVPSGASTGQYE
                     AVELRDGDSNRYLGKGVSRAVDNIHKEIAPALKGRVVFEQAALDRTMIDLDGTPNKGR
                     LGANAILGVSLAAAKAAATNLKIPLYRYLGGPSVSTLPVPMMNIINGGAHADNNADFQ
                     EFMIIPREFPSFKEALRAGAEIFHALKKVLHDKGLATSVGDEGGFAPMVASNQDAMDL
                     IMTAIEKAGYKPGVNVFLGLDCAASEFCKDGKYILEAEAQKEKSPDQLIEYYESLIGK
                     YPLITIEDGLDENDWAGWSKLTQKLGKKVQLVGDDLLVTNTEKLSRAIREGIANSILV
                     KVNQIGSLTETLAAIDMARRAGYTAIISHRSGETEDATISHIAVATGASQIKTGSLSR
                     TDRVAKYNELLRIEEHLEKQANYPGKKAFFSIPGNAV"
     gene            1736..2245
                     /locus_tag="PLG78_15615"
     CDS             1736..2245
                     /locus_tag="PLG78_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24599.1"
                     /translation="MVSAQLTLESLNTVETSVETWIDFCTVSLQSDFYRLTLQKTGSI
                     ERAAALTLLNNYISTFSPSERERLERDAEFFYGYARGFIEELAPFRYNKSGYDPGVRS
                     AFMSKIKALLRAQKNPDGSYKDKERYIFVETIVRICSSLDFIIKVHDDYKEFLFRELP
                     QFRTAPNLK"
     gene            complement(2277..3341)
                     /locus_tag="PLG78_15620"
     CDS             complement(2277..3341)
                     /locus_tag="PLG78_15620"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE24600.1"
                     /translation="MEEQKQQDSLDLSARIVQLLFFAITIFFLFITLYGAKEVSVPIA
                     IAFLITLMLNPLVDLLEGAGLPRILATVIVFLLFIAALYFTVYNIVPLIQDQVRDIVS
                     RWPDIQKKANVFFTDTNAMLAEKLPRFIRIERLRMDTITGFIEEWSKQQGQTMKENLG
                     SIVTNLIITPIITFIFLLQGEEIYLRIIRIVPNRYFEMSLLVAHRVKKQITGYLRGLA
                     VQLLINVSIFSVGFMWIGLDYGFTMGVVAGSLNFIPYAGPVIGIIPALIIALQQGGNV
                     VMLVLVVFACAHLFDNVFTQPVILARSAELHPLIAILALITCERLFGVVGMIIAIPLA
                     GILLVTIEVMYNSLKSFRII"
BASE COUNT          799 a          844 c          890 g          921 t
ORIGIN      
        1 gtttgtttcg aaggtgagaa tggtagctcc gggcgccgag cttcttgcgg ccggagtttc
       61 ttctatcttc gcatactcct cttctagcgt tctgttttcg gtttctaatt cttcgatttg
      121 gcctcggatt gcgtgaaggc ggtcctgcag tttctggcgg gtgaaatatc ctgcttcccc
      181 aaaaagaaag acgtgaagca ggaatgtgat gactaccatc ccgcccaggg cggccagctg
      241 gaccttgagt ctgctcatgg aatcaagatc ggctgaccgg gcgggttgct gaactagact
      301 gcgtttcccg ggatgctgaa gaatgccttc ttcccggggt agttggcctg tttttcgagg
      361 tgttcttcaa tgcgtagaag ctcgttgtac ttggccactc tatctgtgcg gctgagcgat
      421 ccagtcttga tctgggacgc ccccgtggcc accgcaatgt gcgaaatggt cgcatcctct
      481 gtctcaccgg accggtggct gatgatggca gtgtagcctg cgcggcgagc catgtctatg
      541 gcagcaagtg tctctgtgag acttccaatc tggtttactt ttaccagaat cgagttcgca
      601 attccttcgc ggatggcccg ggagagcttt tccgtattcg taacgagaag atcgtctccc
      661 acgagttgaa ctttcttgcc cagcttttga gtgagtttgc tccagccggc ccaatcgttt
      721 tcgtccagac cgtcttcgat tgtgatgaga ggatactttc cgattaagga ttcgtagtac
      781 tcaatcagct ggtccgggct tttttctttc tgcgcttccg cttcgagtat gtatttgccg
      841 tctttgcaga attcggaggc agcgcagtcg agaccgagga acacattcac tcctggtttg
      901 tagcctgcct tttcaatcgc agtcatgatc aggtccatgg cgtcctggtt ggatgcgacc
      961 atgggtgcga atccaccttc gtctccgacc gaagtggcga gccctttgtc gtgcagcact
     1021 ttcttgagtg catgaaagat ttccgcgccg gccctcagag cctccttgaa agaaggaaat
     1081 tcgcgcggaa tgatcatgaa ttcctgaaaa tcagcattgt tgtctgcgtg cgcgccgcca
     1141 ttgatgatgt tcatcatggg gactggcaga gtggaaacgc ttggcccacc gagataccga
     1201 tagagtggaa tcttaaggtt tgttgctgcc gctttggccg ctgccagaga tactcccaga
     1261 attgcgtttg ctcccagtct ccctttgttg ggagttccgt ccaggtcgat catggtgcga
     1321 tcgagtgccg cctgttcaaa aaccacacgg cctttgaggg caggcgcgat ttctttatga
     1381 atgttgtcta cggctctgga tacgcctttg ccaagatagc gattggaatc accgtcgcgc
     1441 aattctactg cttcgtattg gcctgtggac gctccggacg gcacagcggc gcggccaaga
     1501 gtgccatctg cgagaatgac gtctacttcg accgtggggt tgccccggga atcaaggatt
     1561 tcccgtgcat ggatggtatg gatggttgcc tggctttctt tgcttacgat ttgtttcaca
     1621 atcaaatggg aagagcccgc gcgcgccggt aaagcattct tcgtaaaaaa aatcaatcat
     1681 gcgaatggct ttcaattttt ttgattgaaa acctgccgct ggctcgtcca gtgcaatggt
     1741 gtccgcgcag ctcacactgg aatcactcaa caccgtagaa acaagcgttg agacatggat
     1801 tgatttttgt acagtcagtc tacaatctga tttttatcgc ctcactctcc agaaaacagg
     1861 aagcattgaa agagccgcgg cccttacact tctcaataat tacatcagca cgttttctcc
     1921 gagcgaaaga gagcgtcttg agcgagacgc tgagttcttc tacggttacg cgcgcggatt
     1981 tattgaagag ctggctcctt ttcgctacaa caaaagcggc tacgatcctg gagttcgttc
     2041 tgcgttcatg tccaagatca aggctctttt acgcgcgcag aaaaatccgg atggatctta
     2101 caaagacaaa gaaagataca ttttcgttga aacaattgtt cgaatttgct catctctcga
     2161 cttcatcatc aaagtgcacg atgactacaa agagtttctc ttccgagagc ttccacagtt
     2221 tcgcacagct cccaatctga aataatcttc gcgccgcgcc tgcgattcca gcagctttag
     2281 ataattctga aagacttcag tgagttgtac atcacttcga tagtcaccag caaaatgcct
     2341 gccagaggaa tggcgatgat cattccaacc actccaaaca atcgttcgca agtgatgagt
     2401 gcgaggatcg caatcaatgg atgtagttct gcgcttcgcg cgagtatgac cggctgagta
     2461 aaaacgttgt cgaacaggtg ggcgcaggca aatacaacga gcaccagcat cacaacgttt
     2521 ccaccctgct gcagagcgat gatgagtgcg ggtatgattc cgatgaccgg tccagcgtac
     2581 gggatgaaat tgagcgaacc ggccaccacg cccatggtga atccgtaatc cagtccaatc
     2641 cacataaatc caacggagaa aatagaaacg ttgatcaaga gctgcaccgc gagtccgcgc
     2701 aaatagccgg tgatttgctt ctttactctg tgcgctacaa gcaggctcat ttcaaagtaa
     2761 cgattcggga cgatccgaat gatgcgaaga tagatttcct ctccctggag aaggaagatg
     2821 aaagtgatga tgggcgtgat gatcaggttt gtaacaatgc tgccgaggtt ttctttcatg
     2881 gtctggccct gttgcttaga ccattcttct atgaatcctg tgatggtatc catgcgcagc
     2941 cgctcgattc gaatgaatct aggcagtttt tctgcaagca ttgcgtttgt gtcagtgaaa
     3001 aacacattcg ccttcttttg aatgtcaggc cagcgcgaaa caatgtcgcg cacctggtct
     3061 tgaatgagcg gtactatgtt ataaacggta aagtaaagag cggcgatgaa gagcaaaaag
     3121 acgatcacgg ttgcaagtat gcgtggtagt ccggctcctt cgagtagatc caccagagga
     3181 ttgagcatca gcgtaatcag aaatgcaatg gcaatgggga cactgacttc ttttgcaccg
     3241 tagagcgtga tgaaaagaaa gaagatggtg atggcaaaaa acagaagctg cacgatgcgc
     3301 gcagacaggt caagggaatc ctgttgcttc tgctcttcca ttacttatcc tgccagattt
     3361 tctttctcag tttctggttg gagttttcgc tcatttgaga atctagcttc tcgagttcca
     3421 tgtttgcatg aatcagtctt tgtccaatga gctt
//
LOCUS       DAOQJL010000430         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17535_length_3448_cov_4.344533, whole genome shotgun sequence.
ACCESSION   DAOQJL010000430 DAOQJL010000000
VERSION     DAOQJL010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17535_length_3448_cov_4.344533"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..875
                     /locus_tag="PLG78_15625"
     CDS             57..875
                     /locus_tag="PLG78_15625"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNE24601.1"
                     /translation="MADIPDLTSEQFRKFADLIRREASITLREQKIVLLSNRLRRRAL
                     ATGCANFDEYFAFVTSKPEERRPFLEAITTNESYFWRSTASFDLMKKVALPQLLKAYP
                     KAPIQIWSAGCSTGEEPYNIAMEVTEAMKSLGVFPYQVIATDISARVVDFAKVGRYSG
                     RKIEKIPPLLLYRYFRADPESKGQFLVRDDLRTKIDFRVENLFESTPRNIHCIFCRNV
                     MIYFQKADQEILIRRFYDALVPGGFLLVGHSESLHVLQADFTPVHCPDGVIYRK"
     gene            912..1484
                     /gene="gmk"
                     /locus_tag="PLG78_15630"
     CDS             912..1484
                     /gene="gmk"
                     /locus_tag="PLG78_15630"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNE24602.1"
                     /translation="MDRAGHAVVISSVSGGGKTSLIHLLTRMYPHLETVITATSRPIR
                     EGEINGVHYFFYSRDEFEARIARGEFLEHATVHGNHYGVPLDQVRGKMDQGKSVILNI
                     DVQGARRVKEILGDRVITFFLLPPNRPEWERRLRGRGTDSEQVIQHRLAEGLRELEEA
                     RGYDYRIINDVLERAAGEISVILKEREVLH"
     gene            1493..2368
                     /gene="miaA"
                     /locus_tag="PLG78_15635"
     CDS             1493..2368
                     /gene="miaA"
                     /locus_tag="PLG78_15635"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNE24603.1"
                     /translation="MPVLFGPTGSGKTAALCRLTDVYGDKFEVIACDSRQLYRGLEIT
                     SAAPTSDELAIIPHHLISILDPNEETTAFRFRRLAAEAVTDILSRGHVPVLSVGTGFY
                     FKAMRTRTQVSTPDAFVREAIQSMTPSQRLSELSLKRPDLLQKGGGRIHENDDYRIGR
                     ALENLVPGREDLLPEFPCEFLSFYLDISVSDLNERLRLRVLKMIELGLVEELRGVFDR
                     FGKCPGLSLIGFDLVQLLLTGELSANDFAERLWIEHRQYAKRQRTWFRREKIEARGSW
                     ADFVSWVGLFVDPRR"
     gene            2398..2643
                     /gene="hfq"
                     /locus_tag="PLG78_15640"
     CDS             2398..2643
                     /gene="hfq"
                     /locus_tag="PLG78_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNE24604.1"
                     /translation="MANKNNIQDLVLNSARKEKMEITVYLTNGVPLKGRVVSFDNFTV
                     VIEQDSKQSLVYKHAISTIVLSKPLNLDFSEDGAVKG"
     gene            2640..>3448
                     /locus_tag="PLG78_15645"
     CDS             2640..>3448
                     /locus_tag="PLG78_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24605.1"
                     /translation="MISRFFRVFFWAILLSSIFSWIYLGIVSATARQAIIVLHRKRGM
                     PDLVAPGESQFFPGRIFPGRVEIHPVDIGPRMLVVDYKKGLYQTEMLGLDDSFYIRFK
                     FRFRYTLQPEKLPDLFLKMEGLDWKALEPYLQLRLSEMLTKRLLVIFPADNDLGQVPR
                     RLNDYFNGPALEEMKSILGPEGIEIQNLVVENIYVPDAGRYTAILGGGQGILNQKLER
                     IRKRDEALARRDAESLTDEATYARLETIGKLLIRYPGLKDYLAVDRLGSDV"
BASE COUNT          829 a          849 c          888 g          882 t
ORIGIN      
        1 cgggtctttg ccttccatgc cccctgaacc tcgcagaaaa aaattaggcg actgacgtgg
       61 ctgacattcc ggatctcacg tctgagcaat ttcgcaaatt cgcagacctg atcagacgtg
      121 aagcctcgat cactttgcgg gaacagaaaa tcgtcttact atcgaaccgt ctgcgcaggc
      181 gcgccctggc aacaggctgc gcgaatttcg atgagtactt tgcttttgtt acatctaagc
      241 cagaggagcg aaggccattc ctcgaagcga tcactacaaa tgagtcctac ttctggcgga
      301 gcacggccag ctttgatctc atgaaaaaag tcgcgcttcc gcagctcctc aaagcatacc
      361 caaaagctcc cattcagatc tggtccgccg gatgttccac gggagaagag ccttacaaca
      421 ttgccatgga agtaacagaa gcgatgaaat cgctcggcgt gtttccctac caggtgattg
      481 ccacagacat ctcggccaga gtggttgatt ttgcgaaagt gggccgctat tcgggacgca
      541 aaatcgaaaa aattccgccg ttgcttttgt atcgctattt tcgcgcggac ccggaatcga
      601 agggtcagtt tctggtaaga gacgatcttc gcacgaagat cgactttcgc gtagaaaacc
      661 tttttgaatc cacacccagg aatattcatt gtatcttttg tagaaacgta atgatctatt
      721 ttcagaaggc ggaccaggag attctgattc gccgcttcta tgatgctctc gtacctggcg
      781 gctttttgct cgtaggccac tccgaatcgc tccatgttct tcaggctgat ttcactccgg
      841 tccattgtcc ggatggcgtg atctaccgta agtgaaactt gacagcgcca ggccttcttc
      901 aagtatcatc tatggaccgg gcggggcatg cggtggtcat ctcttccgta tcgggaggag
      961 gaaagacctc cctcattcat ttgctcacgc gaatgtaccc gcacctggaa acagtcatca
     1021 ccgctaccag tagacccatt cgagaagggg aaattaacgg agtgcactac ttcttctact
     1081 cgcgtgatga gtttgaagca agaattgctc gcggagagtt cctggaacac gcgactgtgc
     1141 acggaaatca ctacggagtc ccactcgacc aggtgcgcgg caaaatggac cagggaaaaa
     1201 gcgtcattct aaacattgat gtgcagggag cgcgaagagt taaggagatt ctgggagaca
     1261 gagtgattac atttttcctt cttccaccca atcgtccgga atgggaacgc agacttcgtg
     1321 gacgcggaac ggacagcgaa caggtcatcc agcatcgcct cgcggaaggc ttgcgcgaac
     1381 ttgaagaagc gcgcggatat gactacagaa tcatcaacga tgtacttgag cgcgccgccg
     1441 gagaaatcag tgtaatcttg aaagagagag aagttctgca ctgaaaccgg tcattcctgt
     1501 actctttggt cctacaggtt ctggcaagac agccgctcta tgtcgcctga cggatgtgta
     1561 cggagataaa ttcgaagtca tcgcatgcga ttcgaggcaa ctctaccgcg gtcttgaaat
     1621 cacttctgct gcaccaacgt cggatgaact ggctatcatt ccgcaccatt tgatttcaat
     1681 tctggatccg aacgaagaga ctacagcgtt tcgctttcgc aggcttgcgg cagaggcggt
     1741 tacagatatt ctctctagag gccatgttcc agtgttgtct gtcgggactg gtttctattt
     1801 caaagccatg cgcactcgca cacaggtctc cactccagac gctttcgtgc gagaggccat
     1861 tcaatcaatg acaccgtccc agcgattgtc ggaactttct ttgaaacgcc cggaccttct
     1921 tcaaaagggc ggcggacgca ttcatgagaa tgacgattac aggataggca gggcgcttga
     1981 gaatctggtc ccaggacgtg aggaccttct tccagaattc ccttgcgaat ttctttcctt
     2041 ctatctggat atttctgtgt ccgatttgaa cgaacgactt cgtctgcgcg ttttgaagat
     2101 gatcgaactg ggtctggtgg aagaattgcg cggtgtgttt gatcgctttg gaaaatgccc
     2161 ggggctttcc ctgataggat ttgatctggt gcagttgctg cttacaggag aattgagtgc
     2221 caatgatttt gcagagcgtc tgtggatcga gcacaggcaa tatgcgaaga ggcaacgtac
     2281 gtggttcagg cgagagaaga tagaagcacg tggctcctgg gccgattttg tgtcctgggt
     2341 cggattattc gttgacccgc ggcgttaaga tcgcaacctg tatttcggag aaccgccgtg
     2401 gccaacaaaa acaatataca ggatcttgtg ctgaattccg cgcgcaagga aaagatggag
     2461 atcaccgtct acctgaccaa tggcgttcca ctgaaaggca gagtcgttag ttttgacaac
     2521 tttactgtag tcatcgaaca ggattccaag cagagcctcg tatacaaaca tgcaatctct
     2581 accattgttc tctccaagcc gttgaacctg gacttctcgg aggatggcgc agttaaagga
     2641 tgatttcgcg tttcttccgc gtttttttct gggccattct gctctcttcg atcttcagct
     2701 ggatctatct tggcattgtt tctgcgaccg cgcgtcaggc catcattgta ctgcaccgca
     2761 agcgcggaat gccggatctg gttgctccag gtgaaagtca gttctttcca ggtagaatct
     2821 ttccaggccg cgttgagatt catccagtgg acattggccc tcgcatgctc gttgtggact
     2881 acaagaaagg cctttaccag accgagatgc tcggtctcga tgattcgttt tacataagat
     2941 ttaagttccg tttccgttat actctccagc ctgagaaact tccagatttg tttttgaaaa
     3001 tggaaggtct ggactggaaa gccctggaac cgtatcttca acttcgcctc tcagagatgc
     3061 tcacaaagag acttcttgtg atctttcctg cagataacga tctgggccag gtgccgcgtc
     3121 gtctgaacga ttacttcaat ggcccggctc tcgaagaaat gaaatcaatc cttggaccgg
     3181 aagggattga aattcagaat ctggtagttg agaatattta tgttcctgat gctggacgtt
     3241 atacggcgat tcttggtggc ggtcagggaa tcttgaatca gaaactggaa cgcattcgca
     3301 aaagagatga agctcttgcg cgtcgcgacg ccgaatcttt gacggatgaa gccacttatg
     3361 cgcgactgga gactatcggc aaattgctga ttcgctatcc tggtctcaaa gattacctgg
     3421 ctgtggatcg ccttggtagc gatgtgaa
//
LOCUS       DAOQJL010000431         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17545_length_3446_cov_5.216160, whole genome shotgun sequence.
ACCESSION   DAOQJL010000431 DAOQJL010000000
VERSION     DAOQJL010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_3446_cov_5.216160"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLG78_15650"
     CDS             complement(<1..1096)
                     /locus_tag="PLG78_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE24606.1"
                     /translation="MARFACVKSSAGAHFFFPARDTVPMVSLARAFVLASFVFTFASL
                     FAAPVRLPWNSRSQEFGASLSDRDNLYFYSNRGGSGTDLYCSRKVNGVFEPPELLKEL
                     NSSFDDQSPFILGDESGIIFSSNRDGSHEFRTARGIAVSRDLYYARHTESGWDEPEIM
                     PETINTDLIEENPFLYGKRLFFVRYPFGKPELAHIYYSDLIESGWSEARPLFSFSAIT
                     PGVFRGRFFFATRAQSGKYQIQSIPLDQILSPDAKNLIRAEPGLDPQSDEASYAESRD
                     GTVVFARRSPTGDYDLFSMNQDPWESSENFSLTSILFESSRSDVLPESSPILDRLAEY
                     LKRTQAKVFVTGHTDRTGDPQANLQLSRDRA"
     gene            1197..1811
                     /locus_tag="PLG78_15655"
     CDS             1197..1811
                     /locus_tag="PLG78_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNE24607.1"
                     /translation="MKKMRPLPTLIFASRWLQLPLYLGLILAQAVYVVHFWIELAHLV
                     EAAMGSAAAVKEIAHSVNMVSPEGEGPTHLNETLIMLVVLGLIDVVMISNLLIMVIVG
                     GYETFVSRMHLEEHPDQPEWLSHVNASVLKVKLATAIIGISSIHLLKTFINSAQYDAK
                     TLIAQTGIHFVFLLSAIAIGLSERVSGGSHAPKHEATGDGKRHH"
     gene            1873..2757
                     /locus_tag="PLG78_15660"
     CDS             1873..2757
                     /locus_tag="PLG78_15660"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNE24608.1"
                     /translation="MKRILLLCLLLSSPLLAEKLQSFEKDNLFGFKNAAGKVVIPARF
                     VMAGEFNAFGFATVIEGSKPYLIDAKGTRKFEMFMFDNGPDYFEEGKARYIEKGKMGF
                     FDEQGRKVTPAAFDFAFPFEGGMAVICNGCKRQEEGEHYRMTGGKWTLIDKTGKMLTP
                     AVFDSAPVMEGNALTGFVDGKKQTVKIRSDAKEAANCKIEARNIEPCNGKTIQVEGIL
                     SNKGQQHPRIALNEKESYWEVFGSDWILVSRDEIHCPGAILATGRLRTKVGPCDAKSS
                     TKNMYCGTALYVDRWQCK"
     gene            complement(2781..>3446)
                     /locus_tag="PLG78_15665"
     CDS             complement(2781..>3446)
                     /locus_tag="PLG78_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24609.1"
                     /translation="IDGELSNCKLPAARVLWQAALDIPFTREDFETAMRDDSFRREIT
                     SHLDMNLPEERAGLFRLVQSGEDGEAAIRRLESLEWTAQGQELEAIARQYMPVQHSVF
                     SSSDYGRRASILKILHDQKASPQAASVLSVFESEIPKTPSERLAEIRIAQVVLGHHEY
                     ALSAARALKPTSRHHLTGVKDAGDLVYFGLGLAGCNDKEIRDAYYSSRNASDQDRGKL
                     CTK"
BASE COUNT          936 a          863 c          865 g          782 t
ORIGIN      
        1 gggcccgatc acgagaaagc tgtagatttg cttgtggatc tcccgtgcga tcggtatggc
       61 ccgtgacaaa gaccttcgct tgcgtcctct tgagatattc cgccaggcga tccaggatcg
      121 gcgatgattc tggaaggaca tcgctgcgcg agctctcaaa gagtatgctc gttaaagaaa
      181 aattctccga agattcccat ggatcctgat tcatgctaaa aagatcatag tcgcctgtgg
      241 gcgagcgtct tgcgaaaaca acggttccat ctctggattc tgcataggac gcttcgtccg
      301 attgcggatc gaggcccggc tctgcgcgta tcagattttt cgcatcgggg gatagaatct
      361 gatcgagagg aatggactgt atttggtact ttccgctttg tgctctcgtg gcaaaaaaga
      421 accggccgcg aaacacaccc ggagtgatgg cgctaaaaga aaaaagagga cgagcctcag
      481 accagccact ctcaattaaa tccgaatagt aaatatgtgc aagttctggc ttgccgaatg
      541 gatatcgcac aaagaataaa cgcttaccgt acagaaacgg gttttcttct atcagatccg
      601 tattgattgt ttcaggcatg atttctggct catcccagcc tgactccgtg tgacgcgcgt
      661 agtagagatc gcgcgaaact gcgatccctc tggcagtgcg aaactcgtgc gacccgtcgc
      721 gattggaact gaatataatt ccgctttcgt cccccagtat gaaagggctc tggtcatcga
      781 aggaagaatt cagttctttc aaaagttccg gtggctcaaa aacaccgttc actttcctgc
      841 tacagtacag atccgtgcct gatccgccac gattagaata gaagtaaaga ttgtcgcgat
      901 cactgagcga agccccgaac tcctgggagc gagaattcca cggaaggcgc actggcgcgg
      961 caaaaagaga cgcgaaggta aaaacaaaag aagccaggac aaaagcgcga gcaagggaga
     1021 ccatgggtac agtatcgcga gcaggaaaaa aaaagtgagc cccggctgaa gatttaacgc
     1081 atgcaaagcg ggccacaggg gaaaaaaaga tcgacaggtt tcgcacaggc tagcgccttt
     1141 ccgtccatcg ccttgcgcga aatcctcccc aaaaaaacaa aaaaggaacc caatccatga
     1201 agaagatgcg cccgttgccc actctaatat tcgccagccg ctggttgcag ctccctctgt
     1261 atctaggttt gatcctcgca caggcagtct acgtggtgca cttctggatt gaactggctc
     1321 atctggtaga agctgcgatg ggatccgcag ctgcggtcaa agaaatcgcg cattcagtga
     1381 acatggtttc tccggaaggg gaaggaccca cccatctcaa tgaaacactc atcatgctgg
     1441 ttgtgctggg tctcattgac gttgtgatga tttcaaacct gctcatcatg gtgatcgtgg
     1501 gtggatatga aacattcgta tctcgcatgc atctggaaga acatccggac cagccagagt
     1561 ggctgtcgca cgtgaatgcg tccgtcctca aggtgaaact ggccactgcc atcatcggta
     1621 tctcttccat ccatcttctg aaaacgttca tcaactctgc ccagtacgat gcaaagactc
     1681 tcatcgcaca gactggaatt cattttgtgt tcctgctgtc tgccattgcc attggacttt
     1741 cagaaagagt gtccggtgga tcacatgcgc ccaaacacga agcgacaggg gacggaaagc
     1801 gtcaccactg attcttataa caggcgacgg aaaatggttg aaccacatca atcattttcc
     1861 gtccaatgca ttatgaaacg cattctgctt ctttgtctcc ttttatcctc ccctctccta
     1921 gcagaaaagc ttcaatcatt tgaaaaggac aatctgttcg gattcaagaa cgctgccggc
     1981 aaagtagtca tccctgcgcg attcgtgatg gcaggcgaat tcaacgcatt tggatttgcg
     2041 actgtaatag aaggaagcaa gccctacctc attgatgcaa agggaactcg taagtttgaa
     2101 atgttcatgt tcgacaacgg accagactac ttcgaagagg gaaaagcccg ttatatagaa
     2161 aaaggaaaga tgggattctt cgacgaacag ggccgtaagg tgactcccgc cgcttttgac
     2221 ttcgcatttc cattcgaagg cgggatggct gtgatctgca atggctgcaa aaggcaggaa
     2281 gaaggcgaac actacagaat gacaggcggc aaatggacgt tgatagacaa aacaggcaaa
     2341 atgcttaccc cggctgtctt tgattcagca ccagttatgg agggaaacgc actcaccggt
     2401 tttgtagacg gaaaaaaaca aacagtcaag atccgttctg atgcgaaaga agcagcaaac
     2461 tgtaaaatag aagccaggaa tattgaacca tgcaacggca aaacaatcca ggtcgaaggt
     2521 attctgtcga acaagggaca acaacacccg cgcattgcgc tgaatgaaaa ggagtcctac
     2581 tgggaagtat tcggcagcga ctggattctt gtatccaggg atgaaatcca ttgccccggt
     2641 gcgatactgg ccacaggaag actgcgcacg aaagtaggtc catgtgatgc gaaatcttcc
     2701 acaaagaaca tgtactgcgg cacggcgctc tatgtggatc gatggcagtg taagtaattc
     2761 aacctttgat tcgtgaacca ttacttcgtg caaagcttgc cacgatcctg atcgctcgca
     2821 ttgcgcgatg aatagtacgc atcccggatt tctttatcat tgcaccccgc aagacctaga
     2881 ccaaaataaa ctagatctcc cgcatcttta acaccggtta aatgatgcct cgatgtaggc
     2941 ttgagtgcgc gtgccgcact gagagcatat tcatgatgac ccagaaccac ctgagcgatt
     3001 cgaatttcag ccaggcgctc cgaaggagtc tttgggattt cggattcaaa cacagagaga
     3061 accgacgcag cctgcggcga tgctttttga tcgtgcaaga tttttagaat ggaggcacgt
     3121 ctaccgtagt cgctgcttga aaaaacagaa tgctgcacag gcatgtactg tctggcaatt
     3181 gcttcaagtt cctggccctg ggcggtccac tccagagatt ccagcctccg gatcgccgct
     3241 tctccgtctt cgccagactg aaccaggcga aatagccctg ccctctcctc cggaagattc
     3301 atgtccagat gggatgtgat ttcacgacga aacgaatcgt ctcgcatggc agtttcaaag
     3361 tcctcccggg taaacggaat gtcgagggcc gcctgccata gaaccctggc ggcgggcaat
     3421 ttgcagtttg atagctcacc gtctat
//
LOCUS       DAOQJL010000432         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17559_length_3445_cov_3.492625, whole genome shotgun sequence.
ACCESSION   DAOQJL010000432 DAOQJL010000000
VERSION     DAOQJL010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17559_length_3445_cov_3.492625"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1369
                     /locus_tag="PLG78_15670"
     CDS             131..1369
                     /locus_tag="PLG78_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24610.1"
                     /translation="MSEERPQEIDFASARDQIEKACDAAGELPAFTFEKGETRFYFSP
                     RNLDRAAVYNAMETVKAHIENVRVHTFESGFVVLQGMGDLYAPRAITSGIKFKFMSVG
                     GVPRIEVTRKGNFIQEEIQACIALFRNFHAAQGAGDPAAILGELGAQVYVPPSGSQDP
                     WTWMAGYEQTKSEVRESIVLPLKNGDLFREVARLARGSDQGVMPRAILFEGPPGVGKT
                     TMARIIATDTGIPLIYVPIENILSKYYGESAQNLSRIFDAAAMMKQAILFLDEIDSLA
                     GSRDEGMFEATRRVLSVLLRKIDGLDTVDGIITLGATNRSGDLDHALLSRFDHSIRFP
                     LPGREEIRAIYRTYAKHLKSEELIDLAAVSEGLSGRNIRDICEYAERRHARILLSTPG
                     SAASAPPASVYLEIARSRNV"
     gene            complement(1605..2204)
                     /locus_tag="PLG78_15675"
     CDS             complement(1605..2204)
                     /locus_tag="PLG78_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE24611.1"
                     /translation="MKYFLFDLMDTIVVDPFHNLIANLLPPGMSFDEWRSCRERAAFE
                     DFEKGLISESEYFHRFYLQDLPESVKARLMRPQLLKTKLYEKIEFVHGMHDLLVDLKG
                     KATLAILSNYGPWYSEVLRLRPELEDLFDFLFFSCEIGHRKPEPGFYGAVHEALRLEG
                     RDAVFIDDREENLTEAARLGWKTHRFAGTKGLSQWISSL"
     gene            2173..>3445
                     /locus_tag="PLG78_15680"
     CDS             2173..>3445
                     /locus_tag="PLG78_15680"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNE24612.1"
                     /translation="MSIKSNRKYFTAEAEPGRCIRHEDFMIFYFLYNALLVLLWPIYQ
                     LLSLFVPRVRQFRLSRNEVGPQSFLGSDGQPVFWLHAASVGEMDQALAIARELKRRGG
                     CKIALSVFSLSVKKLQQPDVDFMFRAPVDFPWAWKRWVKRISPAAFVTMTWDVFPNLL
                     ASLRAARVPSYLACAALSHDSSRLKFPLKQLLGSVYNKLSGIGAVDLENAERFRAIAG
                     STDVRITGDSRYDAIFHKIQTTKLDSDTQRRLSSQGRTWILASTYRACDQKIFPELPA
                     LLRDFEDLRVLVFPHFVEEERLLEVEEGLRAASIESRRFSDPDISGARVVIVDRMGIL
                     ALAYRLADFCYVGGGFHHRIHNTGEPAALGLPILTGTRIETSPIALLLEKEGALYRCE
                     DGRQIIRHVRDFLDRKDYASSRGRAGQKAIEQ"
BASE COUNT          790 a          882 c          919 g          854 t
ORIGIN      
        1 tccttctctg gcagtcgcag caggacaggc ctgcatcgca atgcgttcga ttgaacgtta
       61 tctgcgatcg aaaccttcct tgctgcccaa tcggaattga cctggaaagc gggcgaggca
      121 ggtagatcgt atgtctgaag agcggccaca ggaaatagac ttcgcatccg cgcgtgatca
      181 gattgaaaaa gcgtgcgacg cagcaggcga gttgcctgca ttcacctttg aaaaagggga
      241 gaccaggttt tacttcagtc ctcgcaatct ggatcgtgct gcagtctaca acgcgatgga
      301 gactgtaaag gcgcacattg aaaacgttcg agtgcacaca tttgaatcag gttttgtggt
      361 gctccaggga atgggagatc tttacgcgcc gcgcgccatc accagcggaa tcaaattcaa
      421 attcatgagc gttggtggtg tgccgcgcat cgaagtaaca cgcaaaggaa attttattca
      481 ggaagagatt caggcatgca ttgcgttgtt tcgtaacttt cacgcagcgc agggtgcggg
      541 agatccggcc gccatcctgg gtgaacttgg cgcgcaggtg tatgttcctc cttcgggaag
      601 tcaggatccg tggacctgga tggcagggta tgaacagacc aagagtgaag tgcgcgaaag
      661 cattgttctt ccgttaaaga atggagatct attccgagaa gtggcgcgtc ttgcgcgtgg
      721 ttctgatcag ggtgtaatgc cgcgcgcgat tttgtttgag ggcccacccg gtgttggtaa
      781 gacaaccatg gctcgcatca ttgcgacaga taccggcatt cctttgatct acgttcccat
      841 tgaaaacata ctcagtaagt actacggaga atctgcgcag aacctttctc gcatctttga
      901 cgcggccgcc atgatgaagc aggcgatcct atttctggac gagatcgatt ctctagcagg
      961 aagcagagac gaaggaatgt tcgaagcaac caggcgagtg ctgtctgtat tgctgcgaaa
     1021 aatagacgga cttgatacgg tcgatgggat catcactctg ggagccacca accggtccgg
     1081 ggatctggat catgccctgc tttctcgctt tgatcattcg atccgattcc ctttgcccgg
     1141 gcgagaagag attcgtgcca tctatcgcac gtacgcgaag catttgaaat cagaggaact
     1201 cattgacctg gcagcggtaa gcgaaggcct ttcgggcaga aacattcggg acatctgtga
     1261 gtacgcggag cgtcgccatg cgcgcatctt gctttctact ccggggagcg ctgcgagtgc
     1321 accaccggct tccgtttacc tggaaattgc tcgttcccgc aatgtttgac aatcgtggct
     1381 cgaactgcct ccgcgggctt cttttttctt tattgtgcag tgcaaaacgg gcttgcgccc
     1441 ataggcgacg aaaaaagcat ggcactggca tcggttaatt cgggcatccc cccaaccgcc
     1501 cgaagcctcc tctttgagga ggccgatgct tcgcatgaac actcttcctg cccccggttc
     1561 cccccggggg ctttttttgc cctttttttc gggcgccatg ggcttcaaag gcttgaaatc
     1621 cactgcgaaa ggcccttcgt tccagcaaag cgatgggtct tccatccaag gcgggccgcc
     1681 tccgtgagat tttcttcacg atcgtctatg aacacagcgt ctctcccttc gagacggagc
     1741 gcctcgtgca ctgcaccgta gaatcccggt tccggcttcc tgtggccgat ctcgcaggaa
     1801 aaaaacagga aatcaaagag atcctctaac tcaggacgaa gccgcaaaac ttccgaatac
     1861 cacggaccgt agttggacag aatggccaga gtggcttttc cttttagatc cacgagcaag
     1921 tcgtgcatgc cgtggacgaa ttctatcttt tcatagagtt ttgttttcag aagttgaggg
     1981 cgcatgagcc tggctttcac tgactcaggc aaatcctgta aatagaatct gtggaagtac
     2041 tcgctttcgc tgatcagtcc tttttcaaaa tcttcgaaag cggctctttc gcggcacgat
     2101 cgccattcat caaaggacat ccctggcggc agcagattgg cgatcagatt gtggaacggg
     2161 tccaccacaa tcgtgtccat caagtcgaac agaaaatact tcacggcaga agcagaacct
     2221 ggacgctgca ttcgtcacga agatttcatg attttctatt ttctctacaa tgcgctttta
     2281 gtactgctct ggccgatcta tcagctttta tctctattcg taccgcgcgt gcgtcagttt
     2341 cgattgagtc gcaatgaggt ggggccgcag tctttcctgg gatcagacgg ccaacctgta
     2401 ttctggcttc atgcagccag tgtgggcgaa atggatcagg ctctggctat cgcgcgcgag
     2461 ctaaaacgca gaggtggatg caagattgcg ctgagcgtat ttagcctgag tgttaagaaa
     2521 ctgcagcaac cagacgtaga cttcatgttt cgcgcccccg tagactttcc ctgggcctgg
     2581 aaacgctggg tgaaacgcat ttctcccgca gccttcgtga cgatgacatg ggacgtattc
     2641 ccgaacttgc ttgcatctct ccgtgcggcg cgcgttccat cctatctggc atgtgcggct
     2701 ctttcgcacg attcctctcg tctgaaattt ccactcaaac aattgctcgg atcagtgtac
     2761 aataaactct caggaattgg tgctgtggac ctggaaaatg cggaacgatt ccgcgccatt
     2821 gccgggtcta cagatgttcg cataacaggg gatagtcgat atgatgcgat cttccataaa
     2881 atccaaacca caaaactgga ttcagatacg caacgcaggc taagctctca aggtcgtacg
     2941 tggatcctgg cttctacata tcgcgcatgt gaccagaaga tttttcctga attacctgct
     3001 ctattgcgcg attttgaaga tctgagggtt cttgtgtttc cgcatttcgt tgaagaagaa
     3061 aggcttctgg aagtagaaga agggcttcgc gcggcttcca tcgaatccag aagattcagc
     3121 gatccagata tttcgggcgc gcgcgttgtg atcgtagata gaatgggaat tctagctctt
     3181 gcttatcgcc ttgccgattt ctgttacgtt ggcggtgggt ttcatcatcg cattcacaat
     3241 acaggagagc ctgcagctct cggcctgccc attctgacgg gcacgaggat cgaaaccagc
     3301 ccgatcgcac tgttgcttga gaaagaaggt gcgctctatc gatgcgaaga cggccgtcag
     3361 atcatacgac acgttcgtga ttttctagac cgtaaggatt acgcttcatc gcgtggtcgg
     3421 gccgggcaaa aagcaattga gcagg
//
LOCUS       DAOQJL010000433         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17581_length_3442_cov_4.253322, whole genome shotgun sequence.
ACCESSION   DAOQJL010000433 DAOQJL010000000
VERSION     DAOQJL010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17581_length_3442_cov_4.253322"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLG78_15685"
     CDS             <1..377
                     /locus_tag="PLG78_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24613.1"
                     /translation="KEAPKPAAAAAPAGARNLTPKYSVQVTNELFHNGNVEAWKNIIE
                     SYEGSHPGCKVIVYHEGELIQDLNSLFKWGKVKHGGLLMFQVAGNAIKNVSRLQKYLH
                     EGASKRYETFMKHDMNRNLSLF"
     gene            397..1113
                     /locus_tag="PLG78_15690"
     CDS             397..1113
                     /locus_tag="PLG78_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24614.1"
                     /translation="MFGEHFQKQTDLITRGLKERMDKRGLVLPSKEKDISVYKDFYDF
                     LKEHLPHQFSLATGKVRGKKHILNRSIDCLVYWKLCNKIMEMTGGYILVDHLYSFITL
                     EPELSTQQLITHATMTKGMKALYAATRDIPDNQILPVFSVLFAYRSTIPLLSHKVALE
                     DTAREKEIPINHETDLVCILDQGVIVKDWESGGYRAVETGKDTLMWFYILLMEYLDRD
                     GKVGFDAREYIKSSREYKEY"
     gene            1117..2247
                     /locus_tag="PLG78_15695"
     CDS             1117..2247
                     /locus_tag="PLG78_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNE24615.1"
                     /translation="MLQRISLAIMSLALFAFANCGSNKDYLVVVDTSGSMQEKNTFKR
                     VKDSMPKLLDALKKGDTVTVMRFDTEVQGGSTIKISSDADRAAITKEIESMKAQGSYT
                     DMAKMLLAVREKVKAMKEEGKTAHIVVLSDGLDDPRPEGGRKRGTVDIGKYRDADGGP
                     IQDAFIYYISLGDISNPALEEQLKKLSPQTKTYLAGNRAGDAGKPGSVGKDGKPVQAT
                     PEQAMAEVGSDVSKNDWLSYVKKYWIYAAGLVLLGLLIYLAILLFRKLAEGESLYGTI
                     LYYPEGIQFPNKNTFTLDKVGKAAVTIGARQGNRLRIKDLGTNEVFSFKGKKVGPNLC
                     LKPLGKSARNIQFLSQRQQGLVSPGDRFRLGDYSFIFNDEEA"
     gene            2234..>3442
                     /locus_tag="PLG78_15700"
     CDS             2234..>3442
                     /locus_tag="PLG78_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24616.1"
                     /translation="MKKPENYLAELRQSDRIFRLDRDTILVYTGREERDARPFLRVGA
                     GQSMPPELLKYIENVILSEQEIANAGMELQWMKATLGSGGESVRYVGSKERVSQMYAY
                     AGLAEMDEEDRPPVKVAPYGPFNSRVETKNKCMIMFMATGNFQITLNSSKLLDYFSYR
                     HQKLNIDREYEMITRALHRKERKAEKGTGFIYPGGTLSDKLSLYWFFNRAGLYLHPPE
                     NHHYDLVENSIDPENLKMAMSDSIHAPGFVEFVRRKAIGNTVAGAYLHDMEHAPLLKR
                     IYNQMRWKFFDDGRPLPMADHAVFFKSKTGSHGIFSLKMHEHAEEHVEIMFPLAPVKQ
                     SKSFDFTRGPFDLEFQKIDTKEDLTAGALQGRLVLHAPGRIAPDVYAKGKLRDTMYPI
                     VPGAEYLFHSP"
BASE COUNT          944 a          821 c          890 g          787 t
ORIGIN      
        1 ccaaagaagc cccaaagcca gcggctgcag ctgctccggc cggagcacga aatctgactc
       61 cgaaatattc ggtgcaggtt acaaacgagc tctttcacaa tggaaacgtg gaggcgtgga
      121 agaacatcat cgaatcatac gaaggatcgc atcctggctg taaggtgatt gtataccatg
      181 aaggcgaact gatccaggat ttgaactctc tgttcaaatg ggggaaagtg aagcatggtg
      241 gtctcctgat gttccaggtg gcaggaaatg cgatcaagaa cgtaagtcgt ctgcagaagt
      301 atctgcacga aggtgcttcc aagagatacg agacgttcat gaaacatgac atgaatcgta
      361 atttgagtct cttctagttc gcctgcgaaa agaacgatgt tcggagagca ttttcagaag
      421 cagacggatc taatcaccag aggtctgaag gagagaatgg acaagcgtgg gcttgtcctt
      481 ccttccaaag aaaaagatat ctccgtctac aaagacttct acgatttcct gaaagagcac
      541 cttcctcacc agttttccct ggcaacgggc aaggtgcgcg gaaaaaagca catcctgaac
      601 cggagcatcg actgcctggt gtactggaag ctgtgtaaca agatcatgga gatgaccggg
      661 ggttacatcc tcgtagatca tctttattct ttcattactc ttgagccgga actgtctaca
      721 caacagctca tcacgcatgc aaccatgacc aagggcatga aggctctgta tgccgccaca
      781 agagacatcc cggacaatca gatcttgccg gtgttttctg tgctttttgc ctatcgttct
      841 accattccgc ttctttcgca caaggtagcc ctggaagata cagcgcggga gaaggaaatt
      901 ccaatcaacc atgagacgga cctcgtttgc attctggatc agggcgtaat cgtcaaggac
      961 tgggagagcg gcggttaccg cgctgtggaa acgggcaaag ataccctgat gtggttctac
     1021 atccttctta tggagtatct tgacagggat ggaaaggtag gattcgatgc tagggaatac
     1081 ataaaatctt ccagagaata taaggaatac tgaatcatgc tccagcgaat ctctctcgca
     1141 attatgtccc tcgctctttt tgccttcgca aactgcggaa gcaacaaaga ctatctggtt
     1201 gtagtggata cttccgggtc catgcaggaa aagaacacct tcaagcgtgt aaaagacagc
     1261 atgcccaaac tactcgatgc gctgaagaag ggtgacactg ttaccgttat gcgctttgac
     1321 acagaagtgc agggcggctc cacaatcaaa attagcagcg atgcagatcg cgccgctatc
     1381 acgaaagaaa tcgaaagcat gaaagcgcag ggctcctaca cggacatggc aaagatgctt
     1441 ctcgctgtgc gcgaaaaagt gaaggccatg aaagaagaag gcaagacggc gcatattgta
     1501 gttctttcag atggtctcga tgatccgcgc ccggaaggtg ggagaaaacg cggcaccgtg
     1561 gatattggaa agtatcgcga tgcagacggt ggtcccattc aggatgcatt catttattac
     1621 atcagtcttg gtgacatcag caatcctgct ctagaagagc agttgaagaa actttctcca
     1681 cagacaaaga cttatctggc aggcaatcgc gcgggagatg caggtaaacc aggttccgta
     1741 ggcaaagacg gaaaaccagt gcaggccact ccggagcagg caatggctga agtaggatca
     1801 gacgtaagta agaacgattg gctcagctat gtgaagaaat actggatcta cgctgcaggg
     1861 ctcgtccttc ttggattgct catctacctg gccatcctcc tgtttcgcaa actcgcagag
     1921 ggcgaatcac tctatggaac catcctctac tatccggaag gaattcaatt cccgaacaag
     1981 aatacattca ctctggacaa agtgggaaaa gcagcggtta cgattggggc gcgccaggga
     2041 aatcgtctgc gtatcaaaga cctgggcaca aacgaagtgt tctcattcaa aggcaagaaa
     2101 gttggcccga acctctgcct gaagccgctc ggcaagagcg cacgcaacat tcaattcctg
     2161 tcgcagcgcc agcagggatt ggtgagtccg ggtgaccgat tcagacttgg cgactactct
     2221 tttatattta acgatgaaga agcctgaaaa ttacttagca gagttacgcc agagcgatcg
     2281 tatctttcgt ctggaccgcg ataccattct ggtatacacg ggccgcgaag agcgcgacgc
     2341 acgtccattc ttgagagtcg gcgcaggcca gagcatgccg ccggagctcc tgaaatacat
     2401 tgaaaacgta attctttccg agcaggaaat tgcgaatgct ggaatggaac tgcagtggat
     2461 gaaagccact ctgggttccg gtggagaatc cgtgcgctac gtgggcagca aggaacgagt
     2521 atcgcaaatg tatgcgtatg ccgggcttgc ggaaatggac gaggaagatc gtccacctgt
     2581 caaagttgcc ccttacggtc cgtttaattc tcgagtagaa accaagaaca agtgcatgat
     2641 catgttcatg gctaccggga attttcagat cactctgaat tcaagcaagc tgttggatta
     2701 cttctcctac agacatcaga agctgaacat tgatcgcgaa tatgaaatga tcactcgcgc
     2761 actgcatcgt aaagaacgca aggcagagaa aggtacgggt tttatttatc ccggtgggac
     2821 tctcagcgat aaactcagtc tctactggtt tttcaatcgc gcgggtctgt atctgcatcc
     2881 acctgaaaac catcactatg atctggtaga aaactccatc gatccggaaa acttgaagat
     2941 ggctatgtct gatagcattc atgcgcctgg atttgtagag tttgttcgcc gtaaggcgat
     3001 tgggaacacc gtggccggtg cctaccttca cgatatggag catgcaccgc ttctcaagcg
     3061 tatctacaac cagatgcgat ggaagttttt tgatgacggt cgtcctttgc caatggcaga
     3121 ccatgccgtg ttcttcaagt caaagaccgg atctcacggc atcttctctc tgaagatgca
     3181 tgagcacgca gaggaacatg tggaaatcat gtttcctctg gcgcctgtga agcagagcaa
     3241 gagctttgat tttacccgcg gaccatttga tctcgaattc cagaagatag acacgaaaga
     3301 agatctgacc gcaggcgcac ttcagggtcg tctggtgctt catgcacccg gaagaatagc
     3361 gcctgacgtc tacgcgaagg gcaaactgcg cgataccatg tatcccattg ttccaggtgc
     3421 tgagtacctg tttcactctc cg
//
LOCUS       DAOQJL010000434         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17611_length_3439_cov_3.867317, whole genome shotgun sequence.
ACCESSION   DAOQJL010000434 DAOQJL010000000
VERSION     DAOQJL010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17611_length_3439_cov_3.867317"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLG78_15705"
     CDS             complement(<1..376)
                     /locus_tag="PLG78_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24617.1"
                     /translation="MHQTRIALTLLCACALSFCAQVPQIPEGETDQAFMTGLLTLLYL
                     DSGNCARSYKVGTNSGQLYCSRAPRSICNYWEGFFYLSPSPIYGKIFVSKATQDRYRA
                     DWGFITSDYSTCAASSAAATNLS"
     gene            complement(409..3171)
                     /locus_tag="PLG78_15710"
     CDS             complement(409..3171)
                     /locus_tag="PLG78_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNE24618.1"
                     /translation="MKRLLIVLVFLQSSAAFALPFIGRVLRTQDGQPIADAKVVILET
                     KKSAFTDEQGAFSLDVPETGYYTFRIIDQDKVYQIRKEIRFENEAIAFYAGDPPKEQP
                     AQETVSDGINVVGKRDRTKLSRYTLSNEEIRRFPGVYGDSLKAVQSLPGISPAVPIGV
                     LPSVNLITSSFLQSATGFSIGPPYSNSGSGFLVLRGAGARASQFYLDGFKILYPFHLG
                     DQSSVLNNDIIRNVDVYIGTYPARYGNATGGVIAITGPDEVKKPTGHINVALFQSDAY
                     YEVPFLDTGYFVGTARQSYPNYALLYLYPAAIPSNAKYSQYNDGQFKMGYKFNNNHTI
                     ETIYFGAHDNLKYTQSVAKASNGGASGLSGVGDSISSVSGGAGSVGDSNTDSRPPVGL
                     NRAFDTEGARYIYTTGGAIKNTLTAQISRFKEDFQLDFRSPLTGETIFGYDVQNARHE
                     VQYKDELNLELIRQHLILNLGGEANVHRWELSLKNFSPRSSTNPNTPSFVDTVNQLVE
                     NNRTFRALLDGDRTGYQLNAGFAELEADFWRFRLTPGVRVDHYSLSSSTGVGPRAGAE
                     FKIPETGTTILGAAGRHFNVPANLEQISVEAGNPNLKMEESDHAAAGVQQTLGPLWLV
                     KLEAYKNTFDQVVVADSYVVQPFALRTNRRDLLEKQAELSKQPFEARKLSYSNDGTGW
                     SKGVEFYLKKSRPPGKNGFFGWLSYTWSITKRNNHQPRLTQTDSQRLNSDNNGRTPIA
                     YQEFGKNALIYYNTGETYLVLDNDREELYDPDRTHVGSLVVNYKFNPEWQLGGRWKYS
                     TGLPRTPVIGVQDLNLAILGRLTFIPKYSDFYNSERYPAVHQLDLRLDHFTNYEWGYA
                     NWYVELINVYAHRNVEGENFDFLYPYAKGSNPAYSYESTYIQTPIGGGRLLLLPLINI
                     GLEMKF"
     gene            3231..>3439
                     /locus_tag="PLG78_15715"
     CDS             3231..>3439
                     /locus_tag="PLG78_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24619.1"
                     /translation="MHRAALLLMLLFASSCVTDNVVDSYEAQRRINAAVSAKYKQCNL
                     PVSMLILVPFDEQESDVRACEGEILA"
BASE COUNT          769 a          821 c          935 g          914 t
ORIGIN      
        1 aggaaaggtt cgtggccgca gcagaggaag cggcgcatgt agaatagtcg gaagtgatga
       61 agccccagtc cgcacggtag cgatcctgcg ttgctttgct cacgaatatt ttcccgtaga
      121 tgggagaagg ggatagatag aaaaaacctt cccagtagtt gcagatggat ctgggagcgc
      181 ggctgcaata gagctggcct gaatttgtgc ctaccttgta acttcgtgcg caattgccag
      241 agtccaggta gagcaaggtc agaagtccgg tcatgaatgc ctgatcagtt tctccctctg
      301 gaatttgagg cacctgcgca caaaacgaaa gagcgcaggc gcacagcagg gtgagcgcga
      361 ttcttgtctg atgcactgcg cgctgtgcca gtccgcgacg cgagggattc aaaacttcat
      421 ctccagacca atgttgatca aaggcagaag caagagtctg cctccaccga tgggcgtttg
      481 gatgtacgtc gattcataac tgtacgcagg attcgatccc ttcgcgtaag ggtagaggaa
      541 atcgaagttt tcgccttcta cgtttctgtg tgcgtatacg ttgatcaact ctacatacca
      601 gttcgcataa ccccactcat agttggtgaa gtggtcaaga cgtaaatcga gttgatgcac
      661 ggccggatat ctttccgagt tataaaaatc agaatacttt gggatgaaag tcagccgacc
      721 cagaatcgcc aggttgagat cctggactcc aatgacgggc gtgcgcggca gaccggtgga
      781 gtacttccac ctgccgccga gctgccactc tggattgaat ttgtagttca cgaccaggct
      841 tcctacgtgc gttctatccg ggtcgtagag ttcttcgcgg tcattgtcca ggaccaggta
      901 cgtttctcct gtgttgtaat agatgagtgc atttttgcca aattcctgat aggcgatggg
      961 agttcttccg ttgttgtctg aattcagtct ctgagaatcg gtttgggtga ggcgaggttg
     1021 atggttgttt ctttttgtga tagaccaggt gtagctgagc catccaaaga atccgttctt
     1081 gccggggggc cgtgatttct ttaggtagaa ttctacccct ttgctccatc ctgtgccatc
     1141 gttcgagtag ctgagttttc gcgcttcgaa gggttgcttc gaaagttccg cttgtttttc
     1201 cagcagatct ctgcggttgg tcctgagagc aaaaggttgt actacgtaag aatctgcgac
     1261 aaccacctgg tcaaatgtgt tcttgtatgc ctcaagcttt acgagccaga gcggtccaag
     1321 agtctgctga actccggctg ccgcgtgatc tgattcttcc attttcaaat ttggattccc
     1381 cgcttccacg ctgatctgct ctagattggc ggggacgttg aaatggcgac cggccgcccc
     1441 aagaatcgtt gtgccagtct cagggatttt aaattctgca ccagctcgcg ggcccactcc
     1501 ggtgctgctc gacaaagagt aatggtctac acgcactcca ggtgtaagtc taaatctcca
     1561 gaagtctgct tccagttccg cgaaacctgc attgagctgg tagcctgttc gatctccgtc
     1621 caggagtgca cggaatgtgc ggttattttc aaccagctgg ttgacagtgt ccacgaagct
     1681 cggagtgttc gggtttgtag acgatctggg agaaaagttt ttgagcgaga gttcccagcg
     1741 gtgaacgttc gcttctcctc ccagattcaa aatgaggtgc tggcggatga gttccaggtt
     1801 tagctcgtct ttgtactgta cttcatgacg cgcattttgc acatcgtagc cgaaaatggt
     1861 ttctccagtg agaggagaac ggaagtcgag ctggaagtct tctttgaatc tggagatttg
     1921 cgcggtcagt gtgttcttga tcgctcctcc ggtcgtgtaa atatatcgcg ccccttccgt
     1981 atcaaatgcg cggttgaggc ccacaggcgg gcgcgaatct gtattggaat ctcccacgct
     2041 tccagcaccg cccgaaaccg agctgatgct gtctccgact ccgctgagac cgcttgctcc
     2101 accgttagac gcttttgcca cgctctgtgt atactttaga ttgtcgtgcg cgccaaaata
     2161 gatcgtttcg atagtatgat tgttattgaa tttgtaaccc atcttgaact gcccgtcgtt
     2221 gtattgcgaa tacttggcgt tagacggaat tgcggccgga tacaaataga gcagggcata
     2281 gtttggatag gattgtcgag cggttccaac aaagtatccg gtgtccagaa atggaacttc
     2341 gtagtaagcg tctgattgga agagagcaac attgatgtgg cctgttggtt tttttacttc
     2401 atccggccct gtaatggcga tcacgccgcc tgtggcattt ccgtagcgcg ctggataagt
     2461 gccaatgtag acgtccacat ttcgaatgat atcgttgttc agtacgctcg attgatcccc
     2521 gaggtggaat ggataaagta ttttaaaccc gtcgagataa aactgggaag cccgagcgcc
     2581 tgctccgcga agtacaagga agccgcttcc tgagtttgaa tagggggggc caatcgagaa
     2641 acccgtggcg ctttgcagaa aagaagacgt gatcaggttt acgctcggga gcacaccaat
     2701 cggcactgca ggtgaaatac ctggaaggga ctggactgct ttaagcgagt ctccgtaaac
     2761 gcccgggaat ctgcggattt cttcgttgga gagagtatag cgggatagct tggttcggtc
     2821 tctcttgcct actacgttga ttccgtcaga gacagtttcc tgtgcaggtt gttccttcgg
     2881 aggatctcca gcgtagaagg cgatcgcttc gttctcgaat ctgatttctt tgcgaatctg
     2941 gtataccttg tcttgatcga taatcctaaa ggtatagtat ccagtctcgg gcacatcgag
     3001 agaaaatgcg ccctgttcgt ccgtgaatgc actcttcttt gtttcgagga tcaccacctt
     3061 tgcgtccgca atgggttgtc cgtcctgagt tcgcaacact ctgcctatga atggcagcgc
     3121 gaatgcagca ctggactgaa gaaaaacgag gacaatcagg agtcgcttca tgctgcttga
     3181 cttacaaaaa tggatgccgg ccctctgccg cctaggattt ttggaactag atgcaccgcg
     3241 ccgccctgct tttgatgctg ttgtttgctt cttcatgtgt aacggataac gtcgttgatt
     3301 cgtacgaagc ccagcgcaga atcaatgcag cggtttcagc gaaatacaag cagtgcaatt
     3361 tacccgtgag tatgctcatc cttgttccat ttgacgagca ggaatcagat gtgagagcct
     3421 gcgaagggga gatcctggc
//
LOCUS       DAOQJL010000435         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17641_length_3435_cov_4.231953, whole genome shotgun sequence.
ACCESSION   DAOQJL010000435 DAOQJL010000000
VERSION     DAOQJL010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17641_length_3435_cov_4.231953"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLG78_15720"
     CDS             <1..352
                     /locus_tag="PLG78_15720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNE24620.1"
                     /translation="LSHPLARRRRMSAASLGDFECVSHHPASAIRQAVEKKWKALRLK
                     PRIVMELRSMESVLRSVEAGVGVGFVSRLSVSKKMRVLPISELVSQRDFYFCFRRRSR
                     GMDQFIDLLRNTGS"
     gene            360..1226
                     /locus_tag="PLG78_15725"
     CDS             360..1226
                     /locus_tag="PLG78_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNE24621.1"
                     /translation="MKKVYYIIFSVFCVWVVFAMIPPEKAHAMPVFDSPHFRIAHRCG
                     QPPEATLYSCGKTVEQGLADFIEMDVHLTKDLRLAVIHDEKLDRTTNGTGPVSGLTMD
                     EISRLDAGYKYTADGVTYPFRGKGINIHPLEDFFKAFPNQRFYVEIKTEDLRAVPIII
                     GLIRSHHMENKIVIASVYGPPLAMMEELAPEVARCASFRESLGWVILTKIGLGGVFSF
                     HSHALALPPAGGHWIITRRMIESAKRQNVKIHMWTINDPEAMASWKDAGVDAVMTDKI
                     GLLGDVLTRMQK"
     gene            1223..1984
                     /locus_tag="PLG78_15730"
     CDS             1223..1984
                     /locus_tag="PLG78_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE24622.1"
                     /translation="MIEVKSLTRKYGDLTVLHDVSLKIERGEFVAVTGPSGSGKSTLM
                     GLLAGLDRPTGGQVFLDHEEITSLTEEDLSRVRGQKVGFVFQNFLLVQTLTALENVML
                     PAEIMGRADAKEKAEELLTRVGLAERMHHYPAQLSGGEMQRVAIARAFVNYPPILFAD
                     EPTGNLDSLNGDRIVELLLDLNQTYESTLVLITHNPELAGLSDREFRLRDGKVVQVVA
                     HRRGLRGKASSPNGRSGSRRPVQKRGTRSGSGVRK"
     gene            1981..>3435
                     /locus_tag="PLG78_15735"
     CDS             1981..>3435
                     /locus_tag="PLG78_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE24623.1"
                     /translation="MNLRREIILSLKQTRRSRGKFLFSAMAIVLGVAAISAVRSFSSS
                     LENAVSLESRKLMGADMRLEAGSSIPQNGMTAELLGAGARAADVIEFNTMLRVEKPKP
                     SVPLSTRLVRVRAIASEYPFFGRITSEPESGFARVREGRLPVVLVDSTLLPALGLVEG
                     DSVFLGKQKFRVAGVLRREPGSPGFSAGIGAPVYIHVSFAEKTGLLVTGSRIRYARYF
                     KLPDFFPVEEWKEKHWQEALNANITIYTYNEAASGVRRFMDNLTRFMTLAGLVILLLG
                     GLGIGLAMNVFVRSRMDDIAVMRALGATPGGTMRVFFILGFLTALVGSVLGYAIGFFG
                     ARAGADFGAAYLPVEVHVRFDALSALLSILPGILFTMAFVLFPVFEIRRISPLRVLRR
                     QAEDQTEDGPGLRGFVSKVIRLKYEIISAVVLASVITGVSAGQSESIAVGAFFTSGVA
                     LAVAVLYLLSLGLIRLSRLVMPWIGSYRIRQGIAN"
BASE COUNT          776 a          815 c          937 g          907 t
ORIGIN      
        1 tctttcgcat cctctggcca gaagaagaag gatgtccgct gcttctctag gagattttga
       61 atgcgtatcg catcatcctg ctagcgcgat tcgccaggcg gtggaaaaga agtggaaagc
      121 ccttcgtttg aagccgcgca tcgtaatgga gcttcgcagc atggagtcag tattgcgcag
      181 tgtggaggca ggagtgggcg taggcttcgt gtccaggctc tccgtttcaa aaaaaatgag
      241 ggttcttccg atttctgagc tggttagtca gagagatttt tatttttgtt tccgtcgacg
      301 gtctcgcgga atggatcagt tcattgattt gcttcgcaac accgggagct gagggtttct
      361 tgaaaaaggt atactatata atattctctg ttttctgcgt ctgggtggtt tttgcaatga
      421 ttccgccaga aaaggcgcat gcgatgcctg ttttcgactc tcctcatttc agaatcgccc
      481 atagatgcgg tcagccgcct gaagcaacgc tctattcctg cggcaaaacg gtggagcagg
      541 gtctcgctga tttcattgag atggatgttc accttacaaa agatcttcgc ctggcagtca
      601 tccatgatga aaaattagat agaaccacga acggaacggg ccctgtgtcg ggtctaacca
      661 tggatgagat ctcgcgattg gacgcaggct acaaatacac agcagacggt gtcacctatc
      721 catttcgcgg caaggggatc aacattcatc ccctcgagga tttcttcaaa gcattcccga
      781 atcaaagatt ctacgtggaa atcaagacag aagaccttcg cgcagttccc atcatcattg
      841 gattgattcg ctcgcatcat atggaaaata aaatcgtaat tgcatctgtt tacggccctc
      901 ctctcgcgat gatggaagag cttgctcctg aagttgccag atgcgccagt tttcgcgaat
      961 cgcttggctg ggtaattctg accaagattg gtttgggtgg agtcttctcc tttcattctc
     1021 acgcgcttgc tcttccgcct gcgggagggc actggatcat tacgcgtcgc atgattgaat
     1081 ctgcgaagag acaaaacgta aaaatacata tgtggaccat caatgatccc gaagccatgg
     1141 catcctggaa ggatgccggt gtggatgcgg tgatgacgga taaaattggc ctgctcggcg
     1201 acgttcttac aaggatgcag aaatgattga agtaaagtct ctaacgcgca agtacggtga
     1261 cctgaccgta ctgcacgacg tgtctctgaa aatagagaga ggggaattcg tagccgtaac
     1321 cggcccttcc ggaagcggga aatctacatt gatgggattg cttgcgggac tggacagacc
     1381 caccggcgga caggtgtttc tggatcatga ggagatcacg tctcttacag aggaagatct
     1441 ttcgcgcgtg cgaggtcaga aggttggatt tgtatttcag aattttcttc tggtgcaaac
     1501 gttaaccgct cttgaaaacg taatgcttcc tgctgaaatc atgggtcggg cggatgcgaa
     1561 agaaaaagcg gaggaacttc tgactcgcgt gggacttgca gaaagaatgc atcattatcc
     1621 agcgcagctt tccggcggtg aaatgcagag agttgccatt gcgagagctt tcgtaaatta
     1681 ccctcccatt ttatttgcag atgaaccaac cgggaacctg gacagcctga acggagatcg
     1741 aattgtagaa ctcctcctgg atttgaatca aacctacgag tctaccctgg ttttgattac
     1801 gcacaatcct gaactcgccg ggctttcgga ccgagagttc agactcagag atggtaaagt
     1861 agtacaggtc gtggcgcaca ggcgagggtt gcgcggtaag gctagttcgc ctaacggtag
     1921 aagtgggagt agacgccctg ttcaaaaacg cgggaccaga tcaggaagcg gcgtgcgcaa
     1981 atgaacctcc gccgcgaaat cattttatct ctaaagcaaa cgcgccggtc gcgtggcaag
     2041 tttctgttca gcgccatggc gatcgttctg ggagtcgcag ccatctctgc ggttcgatct
     2101 ttctcttctt cgcttgagaa tgcggtaagt cttgaatcgc gcaaactcat gggtgcagac
     2161 atgcgtcttg aggccgggtc gagcattccg caaaatggaa tgacagctga gcttctgggt
     2221 gctggcgctc gcgctgcgga cgtaatagaa ttcaacacaa tgttgcgcgt tgaaaaacca
     2281 aagccttctg ttccgctgag cacacgcctg gtccgcgttc gagcgatcgc aagcgaatac
     2341 cctttctttg gccgcattac aagcgaacct gaaagcggtt ttgctcgtgt tcgtgaaggg
     2401 cgcctgcccg tggttctggt ggattccact cttcttccag ccctgggact ggtggaagga
     2461 gattctgttt tcctgggaaa acaaaagttt cgcgtagcag gtgttctgcg acgtgagccg
     2521 ggtagtcctg ggttctccgc tgggattggc gcaccggtct acatccacgt gagctttgcg
     2581 gaaaaaaccg gtctcctggt aacagggagc agaatcagat acgctcgata cttcaaactt
     2641 ccagatttct ttcctgtgga agaatggaaa gagaagcact ggcaggaggc gctcaacgcg
     2701 aacattacaa tttacacgta caacgaagcc gcctccggtg tgcggcgttt catggacaat
     2761 ctcactcgat ttatgacgtt agccggcctt gtgattctac ttctcggcgg actgggaatt
     2821 ggtcttgcca tgaatgtatt tgtgcgaagc agaatggacg atatcgctgt catgcgcgct
     2881 ctgggtgcaa ctccgggcgg aaccatgcgt gttttcttta ttcttggctt cttaaccgcg
     2941 cttgtaggaa gcgtccttgg ttatgcgata ggtttttttg gcgcgcgtgc gggtgcggat
     3001 ttcggcgcag cttacttgcc cgtagaggtt catgttcgct ttgatgctct ttctgctctt
     3061 ctttctattt tgcctggaat tctttttacc atggcatttg tgttgtttcc cgtttttgag
     3121 atcagacgaa tttcccctct tcgcgtgctc aggcggcagg cagaagatca gacggaggac
     3181 ggaccaggcc ttcgcggatt tgtgtctaaa gtcatacgtt tgaaatatga aatcatatcc
     3241 gcagtggtgc tcgcatcagt cattactggc gtatccgcag gtcagtctga gtcgatcgcc
     3301 gtaggagctt tctttacttc cggtgtcgcg cttgcagtgg ctgttctcta cctactgtct
     3361 ctaggtttga ttcgcctttc gcgcctcgtc atgccctgga ttggatccta cagaattcgc
     3421 caggggatag cgaat
//
LOCUS       DAOQJL010000436         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_17851_length_3410_cov_4.421759, whole genome shotgun sequence.
ACCESSION   DAOQJL010000436 DAOQJL010000000
VERSION     DAOQJL010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_3410_cov_4.421759"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLG78_15740"
     CDS             complement(<1..298)
                     /locus_tag="PLG78_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24624.1"
                     /translation="MTGLGSLIFATLLEKGEGILLHSLVQFQHERPFDFSTLLDIRIL
                     GLLLAGALVNGIIIRFFAGEVRGSGIDFIIDRFHNHEGRMRGRVATYKSIATIAS"
     gene            complement(431..1240)
                     /locus_tag="PLG78_15745"
     CDS             complement(431..1240)
                     /locus_tag="PLG78_15745"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HNE24625.1"
                     /translation="MNARPLASVLMCHAPVAIPEIAGKNRDAAQKTTSAMKAAASFIT
                     TRSPDVVVIASPHTPRLKEEFVILKGDRLTGDFADFEFSDISVSVPSDQDAVSRLIAE
                     ANKSGIPVSVRYAASLDQGALVPLYFMKKAGWTGKTIVIGFPGRMPLRQFESLGRVIR
                     TVAQTRQETWAFIASGDMSHRLAPGSPAGYDQKAHNFDDSIVLALREGRLADALDVDP
                     ELRKLASEDVVESLAIAYGTSGGMRGGRVLSYESPFGVGYLVAILCDPDSE"
     gene            1294..1992
                     /locus_tag="PLG78_15750"
     CDS             1294..1992
                     /locus_tag="PLG78_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24626.1"
                     /translation="MRILIFCLCVLPTAADLYSQSVRGTARLACSDSLAELRKRKVPL
                     LENANTVFAFGYRQVAGLNQDPVLIRFDSGKRTYCRTDYETTQAEGRIYGLLLAGEDL
                     YAVASLVGPPGKADQDFRRFARKGWMRDSGKGSPRAAIILKIKAQSGDVESAAYLTSV
                     KEDGNSNSVEVLEMDLNSKGLTVKANAWLSPRMTSRAPMQCKGKEPYLQTIIFTPDLS
                     KTVDSFAERCVCCE"
     gene            2051..2989
                     /locus_tag="PLG78_15755"
     CDS             2051..2989
                     /locus_tag="PLG78_15755"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24627.1"
                     /translation="MIRAKKYGTIAAIAIAALLILKFILLDRSPVPETSPEAVNLKLV
                     RALARASRGELPQSLHVLIIAEGAFPKSAVIAGEGLFKKKPMVFDSFQAVFADKKTVI
                     IDTAMDAAGVEKMSKGMPFFADRYAAMQTAMTKAERIILTHEHPDHMGGIGKTEAMDK
                     IKDRLLLTKEQIDDPYRDKSYFTEDNIRAISSVKPLEYDPYFSPAPGIVLIRAAGHSA
                     GSQMIYVALKNGEEYLFVGDIVWSMENLEKLKGKPYLMNAAGFLKEDRAKVAAQIRFL
                     HNLTADPENKVHVVVAHDLEQIKDYIARGIVAEGFQ"
     gene            3068..>3410
                     /locus_tag="PLG78_15760"
     CDS             3068..>3410
                     /locus_tag="PLG78_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24628.1"
                     /translation="MRTFKFSIVIVSILVALTACGGKKSKKGFLLFPFGAGMAVLDAN
                     GNALPENSGTSQQEAPTAGPAQISGSISPMNGSSAQTGFDFSGIEVSLVDPLGNVIAT
                     ATPDSFGVYTFD"
BASE COUNT          864 a          831 c          880 g          835 t
ORIGIN      
        1 gtgatgcaat cgtcgcaatg gacttgtagg tggccactcg cccgcgcatt cttccttcat
       61 gattgtgaaa acgatcaatg atgaagtcaa tcccgctccc gcgcacttct cctgcgaaga
      121 agcgaatgat gattccgttg acgagcgcac ctgccagaag gagccccaga attcgaatgt
      181 ccaggagcgt actgaaatcg aatggacgct cgtgctggaa ttgaaccaga gaatgaagga
      241 ggattccttc ccccttttcc agaagagtgg cgaaaatcag agatcctagt cccgtcaaga
      301 ctccaatcaa aaggcagatg aggtagagag cgcctgaagt tctgataaag gacgggatga
      361 ggcgtgcaac cacagcctac gtcagcgagc ggctgaaaaa acgcaaatga aatgtctttg
      421 cgcaaaatcg tcattccgaa tcagggtcac acaaaatagc aacgaggtag cctacgccaa
      481 aaggactttc gtaggacaac actcttcctc ctcgcattcc gcctgatgtt ccgtaagcaa
      541 tcgccaatga ctcgaccaca tcttctgatg caagtttgcg caactccgga tctacgtcca
      601 gtgcgtctgc cagccttcct tctcttaacg ctagaacaat ggaatcatcg aagttgtgtg
      661 ccttctggtc atagcctgcg ggggatcctg gcgcaagtct gtggctcata tcaccggatg
      721 caatgaaggc ccacgtttct tgccgcgtct gtgcaacggt gcgaatcact cgacccaggg
      781 attcaaattg gcgcagaggc atccttcccg gaaaaccaat gacaatcgtc tttcccgtcc
      841 agcctgcctt cttcatgaaa taaagaggaa ccagcgctcc ctgatcgaga gatgccgcat
      901 aacgtacgct caccgggatt ccactcttgt ttgcttccgc gatcagccgg gaaactgcat
      961 cctgatcgga aggcacagaa accgagatgt ctgaaaattc gaaatctgca aagtcgccgg
     1021 taagtctgtc ccctttcagg attacaaatt cctctttcag tctgggtgtg tgaggggaag
     1081 cgatcacaac tacgtcaggc gatcttgttg tgatgaagga cgctgccgct ttcatggcgg
     1141 atgtagtctt ctgcgctgcg tcgcgatttt tcccggcgat ttcagggatg gcgacgggcg
     1201 cgtggcacat aagaacagat gctagtggcc ttgcattcat ggagggcttg accgttcgtg
     1261 ctctcagttt agatcggatg acgaatggat actatgcgaa ttctcatatt ctgtttatgc
     1321 gttttaccca cggctgcaga tctgtattcg cagtctgtgc gcggaacggc ccgcctggct
     1381 tgcagcgatt ctctggcaga gctcagaaaa cgtaaggtgc ctctgctcga aaacgcaaac
     1441 accgtttttg cattcggcta tcgtcaggtt gcaggactca atcaggatcc agtcttgatt
     1501 cggtttgatt ccggcaaacg tacctattgt agaactgatt acgaaacgac tcaagcagaa
     1561 gggcggatct acggcttgct tctggccggt gaggatttgt acgctgtcgc atctcttgta
     1621 ggacctccag ggaaagcaga tcaagacttt cgccggtttg ccaggaaagg atggatgcgg
     1681 gattcaggaa aaggatctcc cagggcagcc atcattctga aaatcaaagc acagtcaggc
     1741 gacgtggaat ctgcggccta tttgacttct gttaaggaag atgggaatag caactcagtc
     1801 gaagtcctgg aaatggatct gaactcaaaa ggactgacgg tcaaagcaaa tgcctggctt
     1861 tcccctcgta tgaccagtcg cgctcccatg caatgcaagg gtaaagaacc ttatttgcag
     1921 actatcattt tcacgcctga cctctcgaag acagtggatt ctttcgccga acgatgcgtt
     1981 tgctgtgaat gatttacacg gactccggag aaatgctgga aagtaaatgg cacgcccgcg
     2041 tatttgccct atgattcgcg caaagaaata cggcacgatt gcagccattg caattgcggc
     2101 tttactcatt ctaaagttca tcttactgga tcgctcacct gttccagaga catcgcccga
     2161 ggccgtgaat ttgaaactgg ttcgagctct ggcaagggct tcgagaggag aattgcctca
     2221 gagcctccac gtactcatca tcgcggaagg cgcgtttcca aaatctgcag tcatcgctgg
     2281 agaaggactc ttcaaaaaga aaccaatggt cttcgattct tttcaggctg tattcgcgga
     2341 taagaaaacg gtgatcattg atactgccat ggacgcggct ggagttgaga agatgtccaa
     2401 agggatgcct ttttttgcgg accgttatgc ggcaatgcaa acagctatga caaaagctga
     2461 gcggatcatt ctaacgcatg agcatccgga tcatatgggt ggcattggca agactgaggc
     2521 aatggacaag atcaaagatc gtctacttct taccaaagag caaattgacg atccatacag
     2581 agataagagt tatttcacag aggataacat tcgtgcgatt tcttccgtga agcctcttga
     2641 gtatgatcct tatttttctc ctgcacctgg catcgtgctc attcgcgccg caggacacag
     2701 cgcgggtagc cagatgattt atgtcgctct caagaacggg gaagagtatc tattcgtggg
     2761 cgatattgta tggtccatgg agaatctgga aaagctcaag gggaagccat acttgatgaa
     2821 tgcagccggt ttcttaaaag aagatcgtgc gaaagtggcc gcgcaaatcc gatttcttca
     2881 taatctgacc gcggatcccg aaaacaaggt ccacgtggta gtggcgcacg atttagaaca
     2941 aatcaaagat tatatcgcta gaggaatcgt cgccgaaggc tttcaataga acccgttttt
     3001 gggttgccag acaacccttg aaccctaaac tccgggcagt gttcgcaaaa cactgttcgg
     3061 gggggacatg cgtactttca aattctcaat tgttatagtt tctatactag tggcgctcac
     3121 tgcctgtggt gggaagaaga gtaagaaggg gttcttgctt tttccattcg gggcgggaat
     3181 ggcagtgctc gacgcaaatg ggaatgctct tccagaaaac tctggaacct cgcagcaaga
     3241 ggctcccaca gccggtccgg ctcagatttc tggttccatt tctcccatga atggttcatc
     3301 tgcacagacg gggtttgatt tctcaggaat tgaagtttct ctggtggatc ctctggggaa
     3361 tgtgatcgct acggccactc cagatagttt tggggtttac accttcgatg
//
LOCUS       DAOQJL010000437         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18073_length_3384_cov_3.610093, whole genome shotgun sequence.
ACCESSION   DAOQJL010000437 DAOQJL010000000
VERSION     DAOQJL010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18073_length_3384_cov_3.610093"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLG78_15765"
     CDS             complement(<1..446)
                     /locus_tag="PLG78_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNE24629.1"
                     /translation="MDNIKGKVMHVGVAEWKTGKAPDALRTTLGSCVGIVLYSADKKI
                     GGVAHILLGDAPVGKIVHRGKYARPALESLLADLAKLDIKPGMLTARIFGGGSMFEAV
                     HSRFLQSIGPDNVRAATEAVKAHNIPVVAEDTGGTTGRTITLNLEDG"
     gene            508..1722
                     /locus_tag="PLG78_15770"
     CDS             508..1722
                     /locus_tag="PLG78_15770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNE24630.1"
                     /translation="MKSVQPKKNQYSGPVAILEDGKTKVIILGTAHVSPESIADVEKA
                     IKELDPHTVCVELCRPRYDAMLDPDRWRKLNLIRVIREKKMGLLFSSLILSAYQKKLG
                     SEKGIRPGQEMITAVQLAESSGRKLVLADRPVSITLTRAWRKVGFFSKYAIVSQLISS
                     IFVREKVTSEELEQLKAEDALADLFKTLPPRYSSIKDVFISERDLYLAEKIKHAAKEA
                     PGETILAVVGAGHLPGIRAALEAMQPVVLKDLEEVPPANPWTTAFTWLVFSALVGFVI
                     WRMTGQSEQVFKEALAAWILGRSLGAGLGAILSLAHPLTVLVAALVAPFTIFLPGSRV
                     WMFASITEVWLRKPHVEDFEDMTLVSESASGIFKALYKNRVLHLFWVIFLIQMGFLAG
                     NIYIVTFFAKHL"
     gene            complement(1730..>3384)
                     /locus_tag="PLG78_15775"
     CDS             complement(1730..>3384)
                     /locus_tag="PLG78_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE24631.1"
                     /translation="RRQCEDRKSLGKPCTSKQNWTKFIKGRRSSGGMKDKGDINRYAS
                     RVINRIRLFLVLLYTISVATTYSTTPPVQVTAYAIGISLQTSYCLLTFLLDWRKSVPS
                     YWDRLTIVLDVLVLAMVMIVGVTFDTVKTISLLRTTVLYMIYMFYIVCAGLAFSSTGF
                     VLGVGFLCSLACSVVLFVAVQFGGVQLTLNGQLAQQPGYVAWGAEVLKIIFIFVFAVI
                     LRSVFLILDGLRKDAEEQRAQMAESHDALSLQTQKMIAHAENLRASASAIRSVITDFN
                     QRLEGQAAAVQQISATMEEFTASIEHSTESVRTQYQKIDRMNQESAALEKLVSSVDGS
                     SQSLDTQMETARTTGASVASAVDQLSDSLRGIEESFGRVSEVNQIMAEIADRTNLLAL
                     NASIEAARAGELGRGFAIVAQEVGKLADTSSQNAGTIEHIISESARLIETGRHFADQA
                     NAMVKDQGLSLVNMSGGFKNLSGQIGEQSRIIKSLVDALRELKDLSSEIERISREQRE
                     GSSAAAQALASIESHLSTQVERARDLEESIAGIEAGARELEG"
BASE COUNT          843 a          887 c          811 g          843 t
ORIGIN      
        1 ccatcttcca gattgagcgt gatggttctg cctgtggttc cgcccgtatc ttctgccacg
       61 accggaatat tgtgcgcctt gactgcttct gttgcggctc tcacattatc cggcccaatg
      121 ctctgaagaa acctggaatg gaccgcttcg aacatggaac cgccgccaaa aattctagca
      181 gtgagcattc ctggtttgat atctagtttg gcgaggtctg ccaggagaga ttccagagca
      241 gggcgagcgt actttccgcg atggacaatc ttacctacgg gagcgtcccc cagtagaatg
      301 tgcgccaccc cacctatctt tttgtccgct gagtaaagta cgattcccac acaacttccc
      361 agagtggtgc gaagagcatc cggcgctttg cctgtcttcc attcggcgac gcctacatgc
      421 atcaccttgc ctttgatatt gtccaacaac tgatcctcag ctcaaatttc actggagcag
      481 gccagccctg gaagaaaatc tgttccagtg aaatcagttc aacctaaaaa aaatcaatat
      541 tccggtccag ttgcaattct agaagatgga aaaacaaaag tcatcatcct gggcacagca
      601 cacgtttcgc ccgagtccat cgcagacgtg gaaaaagcaa tcaaggagct cgacccccac
      661 actgtttgtg ttgaactgtg tcgccccaga tacgatgcca tgctcgaccc ggatcgctgg
      721 aggaaactca atctcattcg cgtgatccgt gaaaagaaga tgggcctcct cttctcttct
      781 ttgattcttt ctgcctacca gaagaaactt ggctctgaaa aaggaattcg accaggccag
      841 gaaatgatta cggcggtgca gcttgcagaa tcatccggta gaaaactggt cctggcggac
      901 aggcccgtca gcatcactct cactcgtgcc tggagaaagg ttggattttt cagcaaatac
      961 gcaattgttt cgcaattgat cagttccatt tttgtacgcg aaaaagtaac gtctgaagag
     1021 ctcgaacaat tgaaggcaga ggacgcactc gccgatttat tcaaaactct tccgcccaga
     1081 tacagttcca tcaaagatgt gttcatcagc gaacgcgatc tgtacctcgc agaaaaaatc
     1141 aaacacgctg caaaagaagc tcctggagaa accattctgg cagtagtggg agccgggcat
     1201 ttgccaggaa ttcgcgcagc tctagaagca atgcagccag tcgtgctcaa agatctggaa
     1261 gaagtcccac ccgccaatcc atggaccacc gctttcacct ggcttgtatt ctctgcgcta
     1321 gttggctttg taatctggag aatgaccggc caatcagagc aggtcttcaa ggaagcactg
     1381 gcggcctgga ttctgggaag gagtctgggg gcagggctcg gagccattct atctctggca
     1441 catcctttga ccgtgcttgt tgcggcactg gttgcaccat ttacaatctt tctgcctggc
     1501 tctcgcgtat ggatgtttgc ttcgattaca gaagtctggc tcagaaaacc tcacgtagaa
     1561 gattttgaag acatgactct tgtatcagaa tccgcaagcg gcatattcaa agcactttac
     1621 aaaaaccggg ttctccattt gttctgggtg atcttcttga tccagatggg ttttctggcg
     1681 gggaatatct atattgttac tttcttcgcc aaacacctgt aaggcgattt caaccttcta
     1741 attcgcgggc gccagcttca atgcccgcga tgctttcttc tagatcgcga gcacgttcca
     1801 cctgtgtgga aagatgagat tcaatagaag cgagtgcctg agcggccgca gagctgcctt
     1861 ctctttgttc cctggaaatg cgttcaattt cagaagaaag atctttgagt tctcgaagag
     1921 cgtccacgag actcttaatg atgcgtgact gctcaccaat ctgtccggac agattcttga
     1981 aaccgcctga catgttgacc agactgagtc cttgatcctt caccattgcg tttgcctggt
     2041 ctgcgaaatg acgacctgtc tcaatgagcc tcgcactctc tgatatgatg tgctcaatcg
     2101 ttcctgcgtt ctgcgaactc gtatccgcaa gcttgcccac ttcctgcgct acaattgcaa
     2161 agcctcgccc cagttctccg gccctggcag cttcaatcga cgcattgagt gcaagcagat
     2221 tcgtgcgatc tgcaatttca gccatgatct gattgacttc gctgactcgt ccaaatgatt
     2281 cttcgatccc acgcagcgaa tcagaaagct gatccaccgc cgatgcaacg ctcgcgcctg
     2341 ttgtgcgtgc ggtttccatt tgtgtgtcga gggattgact ggagccatct acagaactca
     2401 ccagtttttc cagtgccgcg ctttcctggt tcatacgatc gattttctgg tactgtgtgc
     2461 gaacagattc agtggaatgt tcaatggaag ctgtgaactc ttccatggtc gctgaaattt
     2521 gttgaaccgc agctgcctgg ccttcgagac gttgattgaa gtctgtaatc acagagcgaa
     2581 tcgcgctggc ggatgcacga aggttttctg catgagcaat catcttttga gtttgaagag
     2641 agagagcatc atgcgattcc gccatctgcg ctcgttgctc ttctgcatct ttgcgcaggc
     2701 catccagaat caaaaacacg ctccgcagaa tcacggcgaa aacaaagatg aagatgattt
     2761 tcaaaacttc cgcgccccag gccacatagc ctggctgctg tgccagctga ccgttgagcg
     2821 taagctgcac tccaccaaac tgcactgcga caaagagcac aacggagcac gcaagagagc
     2881 agagaaaccc aactccaagc acgaacccgg tcgaagaaaa tgcaaggccg gcacatacta
     2941 tgtaaaacat ataaatcatg tagagaacag tagtgcgcaa cagggagatg gtcttcaccg
     3001 tatcaaaggt aacgccaacg atcatcacca tggccagcac aagcacatcg aggacaatgg
     3061 tcaggcgatc ccagtaggaa ggcacactct tgcgccagtc gaggaggaaa gtcaaaaggc
     3121 aataggaagt ctgtagagat atgccgattg cgtacgctgt cacctgtacg ggcggagtgg
     3181 tggagtatgt ggtggctaca ctgatcgtat agagcagtac aagaaacaaa cggatccggt
     3241 tgatgaccct ggatgcataa cgatttatgt cgcctttgtc tttcatccca ccgctacttc
     3301 ttcgtccttt aatgaacttc gtccaatttt gcttagaagt gcagggtttc ccgagcgatt
     3361 ttctatcttc acattgcctt ctat
//
LOCUS       DAOQJL010000438         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18084_length_3382_cov_4.531109, whole genome shotgun sequence.
ACCESSION   DAOQJL010000438 DAOQJL010000000
VERSION     DAOQJL010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18084_length_3382_cov_4.531109"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLG78_15780"
     CDS             complement(<1..1207)
                     /locus_tag="PLG78_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24632.1"
                     /translation="MRKFCALWAITLVWSVTRCITTEEVPVARTDTPVRAEMIVKKVE
                     RHLCTTAWGGSCSFQESAILLHTGPVSTREFGYGCLLTRDGVPQAQVVTDKSDGIRIA
                     FRCARNLGWTVVYVGAENKSFKDCKLYNFPGEFRWDEVPGLQSAAARLMLDRCGIPFD
                     WLADEVKARYGQTVLTDLLIATLAAQAPRGSSMDQLQAWDDVYSQLPSEEKKRVASAF
                     RTAILTDSPVLALERAVRYTDLSDPEFLPALLSRMEKIVNSPPHYDTDSASEIILRKL
                     ATVKPDEGARLACRELERERKRGALTFLAGALLAAANGKYNCPVVLSVLEDSSCDAAY
                     YCGEGKICEAKDLASDVERALVGPPGQKLDPNMRDRALLAAALNVEGSNEILKVWHQR
                     HSYRIDQPSQ"
     gene            complement(1280..1693)
                     /locus_tag="PLG78_15785"
     CDS             complement(1280..1693)
                     /locus_tag="PLG78_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin resistance protein BacA"
                     /protein_id="HNE24633.1"
                     /translation="MTEMYVPPGGPPQGPPPSREILQTLGEPYIRKLLQEHYSQLEKS
                     AIRGMFPEDMTEAADRSADFFVQLLGGPPYFSQKYGPPRMRARHMPFAITEAAREIWL
                     GCFKRAMDSLEFPANYREEFERFLVEFSAWMVNRR"
     gene            1737..2180
                     /locus_tag="PLG78_15790"
     CDS             1737..2180
                     /locus_tag="PLG78_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24634.1"
                     /translation="MQKKLRVNFFRAFFLIPAAAFLLPGCQKSDPVTPAVIEKYQADA
                     DLFCNAIVDCMKEEVREKMKATPERRDLVLARMTRDNCRKGQYSLIGRLSTDVAARGA
                     ADEAGIYREYAECAHAVAEAKDCTQRKQLQKEHPACAKMKAESGY"
     gene            2180..3238
                     /locus_tag="PLG78_15795"
     CDS             2180..3238
                     /locus_tag="PLG78_15795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE24635.1"
                     /translation="MQRIDQFCAGFATGDAISNEALLLQKHLQKRGIKSQIFAEQFPD
                     RDAHRVVHIRKFKPKPDSILVYHHSTDTELLSRLPSLDCKKILIYHNVTPAEYVRPYN
                     RGLADRLQHARDVLKEIPHLFQTVLADSAYNASDLERMGYKDVKVMPVALSPIWKTNP
                     QRKNYRFTVLFVGRVFPNKRHQDLLKTFYFLKKIQPDARLRLVGHFHPEMRGYTGELK
                     NLMRELDLRDVEFTGMVSDEQVAQAYADADVFLSMSEHEGFFVPLIESMHFRLPILAY
                     NSSVIPETLGGSGVMFTEKNFPMVAETIVELHENSPFRESILEAQTQRAAAFSLSSTT
                     GIFDSALDQALQGDLPKL"
BASE COUNT          866 a          907 c          844 g          765 t
ORIGIN      
        1 gttgcgatgg ctgatcgatt ctgtaggaat gcctctggtg ccataccttt agtatttcgt
       61 tcgatccttc cacattcaga gccgccgcaa ggagcgcacg atcgcgcatg ttaggatcga
      121 gtttttgccc gggcggtccg acgagtgcgc gctcaacgtc tgaagccagg tcttttgctt
      181 cgcatatctt tccttctccg caatagtaag cagcatcgca ggacgagtcc tcaagaacgg
      241 agagaaccac aggacagtta tacttcccat ttgccgccgc aagaagtgct cccgcaagga
      301 acgtcagcgc tccccttttt ctttctcgct ccagttcacg acaggccaga cgagcgcctt
      361 cgtccggttt cacggtggcg agtttgcgaa ggatgatttc agatgcagag tccgtgtcat
      421 agtgaggagg ggagttgacg atcttctcca tgcgcgaaag aagagctggt agaaattcag
      481 gatctgacag gtctgtatac cgcactgccc gctccagagc aagcacaggc gaatcagtta
      541 gaatcgctgt gcgaaatgca gacgcaacac gcttcttttc ttccgaaggc aattgagaat
      601 acacgtcatc ccatgcctgc agctggtcca tggaacttcc gcgcggagcc tgagcggcta
      661 acgttgcaat caacagatcc gtcaaaacag tctggccata tcgagctttc acttcatctg
      721 caagccaatc gaaaggaatt ccacaccgat ccagcatcaa tctggccgcg gcgctctgca
      781 gacctggcac ctcatcccag cgaaattcgc caggaaaatt atacagcttg cagtctttga
      841 aagatttgtt ctccgccccc acgtaaacta cagtccaacc aaggtttctc gcacaacgaa
      901 aggcaatgcg aatcccatct gacttatctg taactacctg cgcctgcggg actccatccc
      961 gggtgagaag acatccatat ccaaactcac gagtgctcac gggcccggta tgaaggagaa
     1021 tggcggactc ttgaaaggag caggagcctc cccatgcagt cgtacacaga tgcctttcta
     1081 ctttctttac gatcatttct gcgcgcacag gagtgtctgt gcgggccacg ggtacttctt
     1141 cagtggtgat gcagcgggtg acagaccaga ctagagtgat cgcccagagc gcgcaaaact
     1201 ttcgcattct gcagattggc cgctctcgcc tcagggagtg tcaagatgtt tccaaaatcg
     1261 gttttgattg atccgggatt cagcgtcgat tgaccatcca ggccgaaaac tcgaccagaa
     1321 agcgctcaaa ttcttcgcga taattggccg gaaattcgag cgaatccatg gcgcgcttga
     1381 agcatccgag ccagatttcg cgcgccgctt cggtgatggc aaaaggcata tgcctggcac
     1441 gcattctagg cgggccgtat ttctgcgaaa agtacggcgg acctcccaga agctgcacga
     1501 agaaatcagc agatcgatct gccgcctctg tcatatcttc cgggaacatt ccgcggatgg
     1561 cacttttttc gagctgtgaa taatgctcct gcaataactt acgaatatag ggctcgccca
     1621 gggtctggag tatttcacgg gatggaggcg gcccctgtgg tgggcctccc ggcggaacgt
     1681 acatttcagt cataaaacct gcttgcgcta tggtctttgc ggcgcattta cgatgaatgc
     1741 aaaaaaagct ccgcgtgaat ttctttcgcg cctttttttt aatccctgct gccgcgtttt
     1801 tactgcccgg ttgccagaaa tcagaccctg tgacaccggc cgtcatcgag aaataccagg
     1861 cagacgcaga tttattctgc aatgcaatcg tagactgcat gaaagaggaa gttcgcgaaa
     1921 agatgaaggc aactccagaa cgccgggacc tggttctcgc gcgcatgact cgcgacaatt
     1981 gtagaaaagg acagtacagc ctgatcggca ggctgagtac ggacgtcgcg gctcgtggtg
     2041 cagcagacga agccggaatt tacagagaat atgcggagtg cgctcatgca gtggcagaag
     2101 caaaggattg cacacaaaga aagcagctcc aaaaagaaca tcccgcttgc gcgaaaatga
     2161 aagctgaatc cggttactga tgcagagaat cgatcaattc tgcgccggat tcgcgacggg
     2221 agacgccatc tccaacgaag cacttctgct gcagaaacat ctgcaaaaac gcggaatcaa
     2281 atcgcaaatt ttcgcagaac aattcccgga tagagatgcg catagagtcg ttcatatccg
     2341 taaattcaag ccaaaaccag actctattct ggtttaccat cattccacgg atacggaatt
     2401 actttcgcgt ctcccttctc tggactgcaa aaaaatactg atctatcaca acgtaacgcc
     2461 tgcagaatat gttcgcccgt acaatcgcgg gctcgcagac agactccagc acgcacgcga
     2521 tgtgctcaaa gaaattccgc atctgtttca gacagttctc gcagactctg catacaatgc
     2581 ttctgatcta gaacgaatgg gttacaagga tgtgaaagtg atgcccgttg cactttctcc
     2641 catctggaag acaaaccctc agcgcaagaa ctaccgcttc acagtgctgt ttgtgggtcg
     2701 agtgttcccc aataaacgtc atcaggattt acttaaaact ttttactttc taaagaagat
     2761 ccaaccggac gcacgcttgc gactcgtcgg acattttcat ccagaaatga gaggctatac
     2821 gggagagctc aagaacctca tgagggagtt agacctgcga gatgtggaat tcacgggcat
     2881 ggtctcagat gagcaggtgg cgcaggcata cgcagatgca gatgtatttt tgtctatgag
     2941 tgaacacgaa ggtttctttg tccctctcat cgaatccatg cactttcgcc tccccatcct
     3001 tgcgtacaac tcctctgtga tcccggaaac cctgggtgga agcggggtga tgtttacgga
     3061 aaaaaacttc ccaatggtag cagaaaccat tgtggaattg cacgagaaca gcccatttcg
     3121 ggagtccatc ctcgaagcgc agacgcaaag agcagcggca ttctcccttt cctccacaac
     3181 tggcatcttt gactccgcat tggaccaggc ccttcagggc gatctgccta aactttaagc
     3241 acaaagcccg cccagagtac gctcctcggc tgcttcctct gccgaattgt gccaaaaata
     3301 gcggcaaagg tgcagatgca cagaaaatag gaaggtggta ccgaccttgc ataaagaggg
     3361 cagcaatcac tcaggaggat tc
//
LOCUS       DAOQJL010000439         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18135_length_3377_cov_3.947923, whole genome shotgun sequence.
ACCESSION   DAOQJL010000439 DAOQJL010000000
VERSION     DAOQJL010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18135_length_3377_cov_3.947923"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLG78_15800"
     CDS             <1..1145
                     /locus_tag="PLG78_15800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24636.1"
                     /translation="TGMIGSIEVPILWTVFGGLYSLGVFLIARARKLKGLWIPFALIG
                     FTTLPTSIYVISHWVLPSGAATYITGPPSYLYFCMIAVSGFAFDPKISTIAGIVSALQ
                     YVGAFLIDRDHLRITSQDPLMLQDLTSTSIYLFKGMMMVFTGLVVAVLATTAKKLIQG
                     IVAEEKEKFSIKRVLGEYVSPEVADKIVASQKIAEKKQVAILFSDIRGFTSFSEHNEP
                     DAIVERLNEYFSAMVSAIEKNGGTVDKFIGDAVMSVFGGVLPLENPSEAALNAARQMR
                     ASLKELQRGWASTGQQVFDNGIGIHFGEVLQGTLGSEGRKDFTVIGDAVNTASRLEGL
                     CKETNTGILISAQAVERIPESSRVGLRSLGKFQVKGKIKEIEIYGE"
     gene            1165..1809
                     /locus_tag="PLG78_15805"
     CDS             1165..1809
                     /locus_tag="PLG78_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNE24637.1"
                     /translation="MSFLKDLKKNNDRAWFEKHRDRFEAAHEEFSQTAQGLIEQIARL
                     DPGIRPVSLKDSIFRIYRDVRFSRDKSPYKPHFSFFAARGGRKSKYAGYYVHLEPGSC
                     FIGGGIYHPEPENLMKIRTAIAARGEELRRIVQAVPFRKYYKGLEGDRLKNPPRQYAK
                     DHPLAEFLCFKDFIASAKLKDTEVTGDLAGACIMRFRALLPFNRWLSRAVDQDS"
     gene            complement(1769..2185)
                     /locus_tag="PLG78_15810"
     CDS             complement(1769..2185)
                     /locus_tag="PLG78_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24638.1"
                     /translation="MAKKRVLDVGQCRPDHAAIRYVMESLGAEVDAAYLPADALEAMK
                     RERYDLVVINRKLDSDYSDGTDLIRMMKADRSTAEIPVMLISNYPDAQEEAVSLGALP
                     GFGKLEMMKDSVHARIRAALESSVKNPGPRRAKATD"
     gene            2266..3360
                     /gene="ychF"
                     /locus_tag="PLG78_15815"
     CDS             2266..3360
                     /gene="ychF"
                     /locus_tag="PLG78_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNE24639.1"
                     /translation="MSLQCGIVGLPNVGKSTIFNAITRAGAQSANYPFCTIEPNVGRV
                     DVPDTRLTKLSDIVKPKNVVPAAMEFVDIAGLVEGASKGEGLGNQFLSHIRETEAVAH
                     VVRCFTDDNIIHVRGKVSPADDIRIINLELILADLDSLEKQIQKIEKKAKSGDKVEKA
                     RFDLAAKIRDHLNEEKPLRTMSLSDDEKAIAKTFALITARPMLYVMNMDEASASSGNE
                     YTAIVEKIAAEEGAPTVRLCGRTEEEIAALSPEEAQEYLSSLGLEESGLARMIRASYT
                     LLNLLTYFTAGEVEVRAWTVTKGATAPEAAGVIHSDIQRGFIRAEVMSCDDIFKLGSQ
                     QAVKDAGKMRLEGKDYIVQDGDVCYFRFNV"
BASE COUNT          873 a          842 c          867 g          795 t
ORIGIN      
        1 ggacgggtat gattggttct attgaagtgc ccattctctg gactgtgttt ggagggctgt
       61 attctctcgg agtgtttctg attgcccgtg ctcgcaaact caaggggcta tggattccgt
      121 ttgcactcat tggatttacc acgctgccca cctctattta cgtcatcagc cactgggtcc
      181 ttccatctgg agcagccaca tacatcacgg ggccgccttc ctatctttat ttttgcatga
      241 tcgcagtcag cggcttcgcg tttgatccta aaatttccac gatcgccggg atcgtctccg
      301 cccttcagta tgtgggggcc tttctcatag accgggatca tttgcgcata acgtcccagg
      361 atccattgat gttgcaggac ctgacatcta cgtccatcta cttattcaaa ggaatgatga
      421 tggtattcac cggccttgta gtcgcagttc ttgctacgac tgcgaagaaa ctcatccagg
      481 gaatcgtggc ggaggaaaaa gagaaattct ctatcaaaag agtactcggc gaatacgttt
      541 ctcctgaagt ggcagacaag attgtagcgt cccagaagat cgcagaaaaa aaacaggtgg
      601 cgattctatt tagcgacatt cgcggcttca catctttcag tgaacacaac gaaccggacg
      661 cgatcgtaga aaggctcaac gaatatttct cggccatggt gagcgccatt gaaaaaaacg
      721 gtgggaccgt ggacaaattc atcggagatg cagtgatgtc tgtgtttgga ggagtcttgc
      781 ctctggaaaa cccttcggaa gcagctctga acgccgctcg ccagatgagg gcatcgctca
      841 aggaattgca gaggggatgg gccagcacgg gccaacaggt ctttgacaac ggcattggga
      901 tccattttgg agaagtctta caggggactc tcggatcaga gggcagaaag gatttcacag
      961 tcatcggtga cgcggtcaac actgcttcgc gcctggaagg gctctgcaaa gaaacgaaca
     1021 ctggaatttt gatttccgcc caggcggtgg agcgaattcc tgaatcttcg cgggttggtt
     1081 tgagaagtct cggaaaattt caggtgaaag gcaaaatcaa ggaaattgaa atctatggcg
     1141 aataacggta tttctaaagc taccatgtct tttctgaaag atctcaaaaa gaacaatgac
     1201 cgtgcctggt ttgagaagca tcgggatcgc ttcgaggcgg cgcacgaaga gttttcacaa
     1261 acagcccagg gattgattga acagatcgct cgcctggatc ccggcattcg tcctgtgtct
     1321 ctcaaagact ccatatttcg catttatcgg gatgttcgtt tcagcagaga caaatctccc
     1381 tacaaaccgc acttttcgtt ctttgccgca cgcggaggaa gaaaatccaa atacgctgga
     1441 tactacgtgc acctggaacc aggatcctgt ttcattggag ggggcatcta ccatccggaa
     1501 ccggaaaatc ttatgaagat ccggacagcc atcgcagcgc gaggggaaga gcttcgcagg
     1561 attgtgcagg ctgtaccgtt tcgtaagtac tacaaaggat tggaaggaga tcgactcaag
     1621 aatcctccgc gccagtatgc gaaggatcat cctctggcgg aatttctgtg cttcaaagac
     1681 ttcattgcaa gtgcgaaatt gaaagatacg gaagtgacgg gagatcttgc cggtgcttgc
     1741 atcatgcgct ttcgcgctct tctaccgttt aatcggtggc tttcgcgcgc cgtggaccag
     1801 gattcttgac gctgctttcc agggccgcgc ggattctagc atgaactgag tctttcatca
     1861 tctcgagttt tccaaacccg ggcagtgctc ccagagagac cgcttcttcc tgtgcatccg
     1921 gataattcga gatcagcatc accggaatct cagcagtgga gcgatctgct ttcatcatgc
     1981 gaattagatc cgtcccgtct gagtaatctg aatcgagttt gcgattgatg actacgagat
     2041 catacctttc gcgcttcatg gcttcgagcg cgtctgccgg caaataggcc gcatccacct
     2101 ctgctcccag actttccatt acatagcgga tagccgcatg atccggcctg cactggccta
     2161 catcgagaac gcgcttcttc gccatacatt accatgtaaa tcatcgaacg gcagagagtc
     2221 gataaaaact actgttcctg ctatgcatgt caaaaatcgt cggtcatgtc tcttcagtgc
     2281 ggtatcgttg gactgcccaa cgtaggtaaa tctactatat ttaatgcaat cacccgagca
     2341 ggcgctcagt ctgcgaacta tccattttgc accatcgaac caaacgttgg acgagtcgac
     2401 gtgccagaca caagactcac caaactcagc gacatagtaa aaccaaagaa tgtggtgccc
     2461 gcggccatgg agtttgtaga tatcgcaggg cttgtagaag gagcaagtaa aggagagggc
     2521 cttggcaatc agtttctttc tcatatccgc gaaacagaag cagtcgcgca cgtggtccgt
     2581 tgcttcacgg atgacaacat cattcatgtt cgcgggaaag ttagccccgc ggatgacatt
     2641 cgcatcatca atctggaact gattctcgca gacctggatt cgctcgagaa gcaaattcaa
     2701 aagatcgaaa agaaagccaa atccggcgac aaggtagaga aggccaggtt tgatctggcc
     2761 gcgaaaattc gcgatcatct caacgaggaa aaacctcttc gcaccatgag cctttccgac
     2821 gacgaaaagg ccattgccaa aacattcgca ctgatcaccg ctcgccccat gctctacgta
     2881 atgaacatgg acgaagcgtc cgcatcttct ggaaacgaat acactgccat tgtggaaaag
     2941 atcgcggcag aagagggagc gcctactgtt cgactctgcg gaagaacaga agaagaaatt
     3001 gcagctctgt ccccggaaga agcacaagaa tacctttcgt ccctcgggct cgaggaatct
     3061 ggactggcca gaatgattcg agcatcctac accctgctca atctacttac ttacttcacg
     3121 gcgggagaag tggaagtgcg cgcatggact gtgactaaag gagccacagc accggaagcg
     3181 gccggcgtga tccactctga tatacagaga ggattcatcc gcgcggaagt catgtcctgc
     3241 gacgacattt tcaaactggg atcccagcag gcagtcaagg atgcggggaa gatgcgcctg
     3301 gaaggaaaag actatattgt tcaggacggg gatgtttgct atttccgttt caacgtgtaa
     3361 ccatgcaaag gtgagat
//
LOCUS       DAOQJL010000440         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18290_length_3360_cov_3.838427, whole genome shotgun sequence.
ACCESSION   DAOQJL010000440 DAOQJL010000000
VERSION     DAOQJL010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18290_length_3360_cov_3.838427"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLG78_15820"
     CDS             complement(<1..1031)
                     /locus_tag="PLG78_15820"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24640.1"
                     /translation="MKPKKKKENGLRTATYASLASAVISAVASYFVPAYGRAPLDSAL
                     VGALIGFLIPFSLSGIAALAQGGASFKKLPYAGYLVTISLLIAADTLVIYFIIGLLLF
                     PANVLDQSQLILAGGLSAGISVTFSVANVLRQFIGGSVLADIFLGRYHRAKEEIRSFV
                     FIDLVSSTALAESLGSSLFFRMINDFHSVVERATRANKGILYKYMGDGQILVWPGTLE
                     GRTSAILACLEIQRELAELRERFESKYNHAADFTAGLHCGPVLIGEIGDERKEIGYWG
                     DTVNTTQRIQSSCKTYHCSILASEAFMDQIQSLPARLSVAQNVPLRGKSNPVNLIRIE
                     SLTASETDS"
     gene            complement(1028..2155)
                     /locus_tag="PLG78_15825"
     CDS             complement(1028..2155)
                     /locus_tag="PLG78_15825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE24641.1"
                     /translation="MPRWLAAVPEKVRREITNNNLSRAWIAGLVMLPVHFAHVLLFYF
                     RVVQPEEESWRWGIIQSHLLAAAFWIPALPVLRFFMNRRERWIGPAHFLIFAAVIGYV
                     FLGAIVCAVDQQVAPAPMAYFVAVIGVAILLRLPPVETLVIFAAGLIFVWMAVGITQT
                     NPTYRLFVRVNCITASGVGLIVGASLWYYEVRTVEQRLQIEEQNSALETLAVRDPLTG
                     LFNRRRFLEALDLEIARIRRTGQGGAILWLDLDDFKAINDKFGHPAGDAVLLHVAAIL
                     TGCVRVTDTVARFGGEEFIVLLPDTDQKGALETASRILNTLRETTVTFRGAELSVTAS
                     CGAAAIEIIPPDPAGATIQKADTALYRAKKTGKNRVEHSFS"
     gene            complement(2202..>3360)
                     /locus_tag="PLG78_15830"
     CDS             complement(2202..>3360)
                     /locus_tag="PLG78_15830"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24642.1"
                     /translation="KMIADLEKEFPELLTPRELPFSLMQAWWDESNPAVPYAQSTFKT
                     YNQLMLFRVLDLKHNSEFLRKKFEEKMIANGHALIGRGRNLGFETIFPAETRMLQMIS
                     RLARIEGIPDEETIPLLLDQLILTTQTAYRMFDSLFRESLNQTDKILKSVLPEKIADE
                     LKKTGKVAPVHVDSASVLFSDIVGFTRIAEELPPAQLLAELDRTFAHFEAAMKPNHLE
                     KIKTIGDCFMCVGGISERNRLHEVDTVLAALRIQEFMRKYQKSRARTGSPSWKLRIGI
                     HSGPVVAGVLGQERFNYDIWGDTVNVANRMESAGEPDTINISEVTAQAVQEFFILEPR
                     GRIFVKNKGEMEMYFVRGILPELSVNGGSRVPSHEFARRYATKRRAIRAKS"
BASE COUNT          813 a          850 c          850 g          847 t
ORIGIN      
        1 tgcgagtcag tctcactcgc agtgagtgat tcgattcgga tcaggttcac cggattgctt
       61 ttaccgcgta acggcacgtt ctgcgccaca gacagtctgg cgggcaggga ttggatctgg
      121 tccatgaacg cttcggatgc gagaatagaa caatggtaag tcttgcaaga tgattggatt
      181 ctctgagttg tgttgactgt gtctccccag tatccaattt cttttctttc gtctccgatt
      241 tctccgatca gcaccgggcc gcagtgaaga ccggcggtga agtccgccgc atgattgtat
      301 ttgctttcga atcgttcacg cagttccgcc agttctcttt gtatttccag gcatgcgagg
      361 atcgcggagg ttcttccttc gagagtgccg ggccagacca ggatttgtcc atctcccatg
      421 tatttataga gaatcccttt gtttgcgcgt gtagctcttt ctacaactga atggaaatca
      481 ttgatcatgc gaaagaaaag actcgatcca agtgactctg caagcgcggt ggaagataca
      541 aggtcaatga aaacgaaaga tcgaatttcc tctttcgcgc gatggtagcg gcccagaaaa
      601 atatcagcaa gtacggaacc tccaatgaac tgacgcagca cattggccac tgagaaggta
      661 acggagatgc ccgcggacaa acctcccgca aggatgagct ggctttggtc caagacgtta
      721 gccggaaaca aaagaaggcc aatgatgaaa tagatcacaa gagtgtctgc agcgatgagt
      781 agactgatcg tgaccagata acccgcgtaa ggaagtttct tgaatgacgc gcctccctga
      841 gccagagctg cgattcctga gagactgaat ggaattagaa atccaatcaa cgcgcccaca
      901 agtgcagagt cgagcggtgc gcggccgtaa gcaggaacaa agtagctggc cacagcgctg
      961 atcaccgcac tggcgagaga agcgtaggtg gccgtcctaa gcccgttttc ctttttcttc
     1021 ttcggcttca cgaaaacgaa tgctccactc tgttttttcc ggtcttcttt gctctgtaga
     1081 gtgctgtgtc tgctttctgg atcgtggctc ctgccggatc cggaggaatg atttcaatgg
     1141 ctgcggctcc acagctggca gttacggaaa gctcagcccc gcgaaacgta acggtcgtct
     1201 ctcgcaaggt gttcaggatt cgactggctg tttcgagggc tcccttttgg tccgtgtcag
     1261 gcaacagtac aatgaattct tctccaccga atctcgccac agtatctgtc acgcgaacgc
     1321 atccagtcag aatggccgcg acgtgcaaca acactgcatc tcccgccgga tgcccgaact
     1381 tatcattgat cgctttgaag tcgtctagat cgagccacag aatggcgccg ccctggcctg
     1441 ttcttcggat tcgcgcgatt tcaagatcga gcgcttcgag gaaccttctg cgattgaaaa
     1501 gccctgtgag tggatctcgc actgcaagag tttccagagc gctgttttgt tcttcgattt
     1561 gaagccgctg ttccactgtg cgcacttcat agtaccatag agacgcgccc acgatcaacc
     1621 ccactccaga ggcagtgatg caattgactc tgacgaacaa tctgtaagtg ggattggttt
     1681 gagtgatgcc cacagccatc catacgaaga tgagaccggc ggcgaaaatc acaagagtct
     1741 ccactggagg caggcggagt agaatggcca ctcctatgac tgctacaaag tacgccatgg
     1801 gcgctggcgc tacctgctga tccacagcac aaacaatggc gcccagaaaa acataaccta
     1861 tgacagccgc aaatattaga aagtgcgcgg gcccaatcca tctttcgcgc ctgttcatga
     1921 aaaagcgaag cacaggtaat gccggaatcc agaatgcagc cgccaggaga tgtgactgga
     1981 tgatacccca tctccaggat tcttcctccg gttggactac gcgaaagtaa aaaagcagaa
     2041 catgagcaaa atgcaccggc agcatgacca gacccgcaat ccaggcccga ctcaggttgt
     2101 tgtttgtgat ttcgcgccgt actttttccg ggacggcagc cagccagcga ggcatttcgc
     2161 attgaaatgc cggttgcgaa cctgatcaat caaaaaacag ttcagctttt cgcacggata
     2221 gcgcgtcgtt tggtcgcata acgtcgggca aattcgtgcg aagggactct ggaaccaccg
     2281 ttgacagaca attccggcag aatcccgcgc acaaaataca tttccatttc tcctttgttc
     2341 ttaacgaaga ttcttccacg cggttcgaga ataaagaact cttgcacggc ctgcgcggtc
     2401 acttctgaaa tgttaatggt atctggttcc cccgcagatt ccattctgtt tgcaacgttt
     2461 actgtatctc cccagatatc gtaattgaat cgttcttgtc caagaactcc tgcaactacc
     2521 ggtccgctat ggatgccaat tctcagtttc caggagggag atcctgttct cgctctactt
     2581 ttttggtact ttcgcatgaa ttcctggatg cgaagagcgg cgagcactgt atccacttcg
     2641 tgcaatctgt ttctttctga aatcccgccc acgcacatga agcagtcgcc aatcgttttg
     2701 attttctcaa gatggttggg tttcatggcg gcttcgaaat gagcaaatgt gcgatcgagt
     2761 tctgccagga gctgtgctgg aggaagttcc tctgcaattc tggtgaatcc aacaatgtct
     2821 gaaaacagca cgctcgctga atctacatga accggtgcaa cttttcccgt ttttttgagt
     2881 tcatctgcaa tcttttctgg aagaacggac ttgagtattt tatccgtctg gttgagtgac
     2941 tcacgaaaca gggaatcaaa catcctgtat gcagtttgag tagtgagaat gagctgatct
     3001 agaaggagag ggatggtttc ttcgtccgga attccttcga tgcgcgcaag gcgtgaaatc
     3061 atttgcagca tacgcgtttc tgcaggaaag atagtttcaa accctagatt tcttccgcgg
     3121 cctatgagtg cgtgtccatt cgcgatcatc ttttcctcaa acttctttcg cagaaattca
     3181 gaattgtgtt tgagatccag gacgcgaaat agcatcagct ggttgtatgt tttgaatgtg
     3241 gattgcgcat aaggcacggc tggattggat tcgtcccacc aggcctgcat gagggaaaac
     3301 gggagttcac gaggtgtgag cagttccgga aattcctttt ccaggtctgc gatcatcttc
//
LOCUS       DAOQJL010000441         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18418_length_3348_cov_3.473125, whole genome shotgun sequence.
ACCESSION   DAOQJL010000441 DAOQJL010000000
VERSION     DAOQJL010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18418_length_3348_cov_3.473125"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLG78_15835"
     CDS             complement(<1..585)
                     /locus_tag="PLG78_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNE24643.1"
                     /translation="MAQRSLFFNSLDDALEELSTIERFKAVDTTGLWSYYQILTHLAD
                     YLESSMNNFSWMQPLSMRRTIGKANFFKLKQAGKMDPGLANPSTPKVREEGDEKAALQ
                     RLRRMIEEYKKFVGEHPEHPFYGLLTREDWDFLHRIHMAHHLGFVREAKPKPQAKAKP
                     APKSAGKKPAASVKKSSTGKAKKSAKPAKKSAKKT"
     gene            688..2757
                     /locus_tag="PLG78_15840"
     CDS             688..2757
                     /locus_tag="PLG78_15840"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNE24644.1"
                     /translation="MQLKDPLYGLRIQADYRKLRATVEKNTRNPDLDLLKRAFQFANR
                     AHREQRRLSGEPYIIHPLAVGVLLAEQGLDNATICAGLLHDVVEDTAFGEETIQSEFG
                     SEIHALVKAVTKISNVKKDRARAMGMAGPEIKRLKEREAAENIRLMLLATARDVRVIL
                     IKLADKLHNMRTLQFQKPEKVERIANEVMQIYAPIAGRLGMFRVKSELEDLAFMRLNP
                     EGYRVILEGLKQSRGDLEDFLETVRKILKRRLSEIKIEARIDGRAKHMYSIHQKMMNQ
                     DKTLGQIYDLRGIRIIVEEIRDCYGALGIVHTLWTPIHGRFKDYVATPKVNGYQSLHT
                     TVLGPDGRPLEIQIRTRDMDERAEYGIAAHWLYKSGIDGAERHRWLQRLTGLTEYVGD
                     TGEFIEDLKQELDAEEVYVFTPKGDIIDLPVGSTVLDFAFRIHTHVGLRCKGARVNDR
                     MVPLRTELKSGDRVEVLTDKNATPSPNWLRYLQSPRASQKLRAYLRKQQDDEDVQPPE
                     TPTEGTNDSNNVAELRIRRAKTTDPRRVPIEVAGENDIPVRYAGCCTPVPGDRIVGFI
                     TRGRGVTVHRVDCPNLAGESLEKDRLIQVRWEGLTEKYPVQIDIHARDRQGLYLDLVG
                     EITKTHTNILKAEADLPRSQGDLMRARFLIEVEHVDHLRDIIDSVMSVPGVVSVERMQ
                     DAKKQPH"
     gene            complement(2751..>3348)
                     /locus_tag="PLG78_15845"
     CDS             complement(2751..>3348)
                     /locus_tag="PLG78_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNE24645.1"
                     /translation="RLSEEKAALAKKVQETETKARARSADIEVSRTQFKALADAGNLK
                     LKLVDGRVVVVLSADVLFSTGSAELSDKGKAAVSDVAKVCATMKDKRFQVEGHTDNVQ
                     INPVPGFPYGDNWELGSARAVSVVNVMIAAGVNPDRVSAASFADTRPIASNDQPEGRA
                     QNRRIEIILTPDLSQVPGFEYLKEAPPADTKPGEPKPQ"
BASE COUNT          861 a          872 c          860 g          755 t
ORIGIN      
        1 agtctttttg gcacttttct tcgcaggctt tgccgatttc tttgcttttc cagtggagct
       61 ttttttgacg gaagcggcag gcttcttgcc ggcagatttt ggagccggct ttgcttttgc
      121 ctgcggtttg ggttttgctt cgcgcacaaa tccaagatga tgtgccatat gaatgcgatg
      181 caggaagtcc cagtcttcgc gcgtcaggag cccgtaaaac ggatgctctg gatgctctcc
      241 cacgaatttc ttgtactctt cgatcatgcg acgcagtctc tggagcgccg ccttttcgtc
      301 cccttcctcg cgcaccttgg gagtggacgg gttggcaagc cccggatcca tcttacccgc
      361 ttgcttcagt ttaaagaaat tggcctttcc gatggtacgg cgcatggaca gaggctgcat
      421 ccagctgaaa ttgttcatgc tggattccag ataatccgcc aggtgcgtca gtatctgata
      481 ataggaccac aaaccggttg tgtccaccgc cttgaaacgt tctatagtgg aaagctcctc
      541 tagagcgtca tccaggctgt taaaaaaaag gcttctctgg gccatagcaa ttcttaaggg
      601 cccattggat agaactgcgt ccagccatat agcatttttc tggttgaata gaatgaaaac
      661 atcttatatg ttcaccagaa gacggccatg caactgaaag atccgctata cgggctcaga
      721 attcaggccg attaccgaaa actccgcgcc acagtggaga aaaatacgag aaatccggac
      781 ctcgatctgc tgaagagggc cttccaattt gcaaacaggg cacacagaga gcaaagaaga
      841 ctctccggag agccttacat catccacccg ctcgcagtcg gcgtgcttct cgccgaacag
      901 ggactggaca atgcaaccat ttgcgcgggg ctactccatg acgtagtaga agacactgct
      961 tttggagagg agaccattca gtctgaattc ggctctgaga tccacgccct ggtaaaagca
     1021 gttactaaaa tttctaatgt aaagaaggat cgggcgcggg ccatgggaat ggcagggccg
     1081 gaaatcaaaa ggctcaaaga aagggaagct gcggaaaata ttcgcctcat gcttctggca
     1141 accgcgcgag acgttcgagt catcctgatc aagcttgcag acaaacttca caacatgcgc
     1201 acactgcagt ttcagaaacc tgaaaaagtt gaacgcattg caaatgaggt catgcagatc
     1261 tatgcaccaa tcgccggacg acttggtatg ttccgagtca aatcggagct cgaagatctc
     1321 gcattcatgc gactcaaccc ggaagggtat cgggtcattc tagaaggact caaacaaagc
     1381 cgtggggatc tcgaggattt cctcgaaacc gtccgcaaaa ttctcaaacg ccgactgagc
     1441 gaaatcaaaa tcgaagcgcg catagacgga cgtgcgaaac acatgtactc cattcatcaa
     1501 aagatgatga atcaagacaa gactctcggt cagatttatg atttgcgcgg cattcgcatc
     1561 atcgtagaag aaatcagaga ttgttacggt gcgctgggca tcgttcatac actctggact
     1621 cccattcatg gtagattcaa agactacgtg gccacaccga aagtaaacgg ataccagagt
     1681 ctgcatacaa ccgtacttgg tccggatgga cgtcccctgg agattcaaat tcgcacgcgc
     1741 gatatggacg aaagagcgga atacggaatc gcggcgcact ggttgtacaa gagtggaata
     1801 gacggagcag agagacatcg ctggttgcag cgcttgaccg gcctcacgga atatgtggga
     1861 gacacaggcg aattcataga agatctcaag caagaactcg atgcagagga agtgtacgtg
     1921 ttcactccga agggtgacat catcgatcta ccggttggtt cgactgtgct tgattttgca
     1981 tttcgcatcc acacacacgt tggtctcaga tgcaagggcg cgcgagtcaa tgatcgcatg
     2041 gttcctcttc gcactgaact aaagagtgga gatcgcgtag aagtactcac ggacaaaaac
     2101 gcaacccctt ctccgaactg gctcagatac cttcagtctc cgcgagcttc gcaaaagctg
     2161 cgggcatacc tgcgcaaaca gcaagatgat gaagacgtgc agccgcctga aactccaacg
     2221 gaaggaacaa acgattcgaa caatgttgcg gaactgcgaa ttcggcgcgc gaaaacaacc
     2281 gacccgagac gcgttcccat tgaagtggca ggagagaacg acatccctgt ccgctacgca
     2341 gggtgctgca cacccgttcc cggagataga atcgtcggat tcatcactcg tggtcgcgga
     2401 gtcacagtac atcgagtgga ctgcccgaac cttgcaggag aatcgctgga aaaagacaga
     2461 ctcatccagg tacgctggga aggactcact gaaaaatatc cggttcaaat cgatattcat
     2521 gcacgggata gacagggcct gtacctggac ctggtgggtg aaatcacaaa aactcatacg
     2581 aacatactga aggcggaagc agatctaccc cgctcacagg gagatctcat gcgcgcgcgc
     2641 ttcctcatag aagtggaaca cgtggatcat ttacgcgaca tcatagatag cgtaatgtcc
     2701 gtgccaggtg ttgtgtctgt cgaaagaatg caggacgcga aaaagcagcc tcactgaggc
     2761 tttggttcgc ctggtttcgt gtccgcaggc ggagcttctt tcaagtattc aaatcctgga
     2821 acctgcgaaa gatccggcgt aagtataatt tcaatccttc gattttgcgc gcgtccttcc
     2881 ggctgatcgt tagacgcaat cggacgagtg tccgcgaaac tcgcggcgct cacccggtca
     2941 ggattcactc cagccgcaat catgacgttg accacactga ccgctcgcgc agaccccagc
     3001 tcccagttgt ctccgtaagg aaagccaggc acagggttga tctgcacatt gtccgtgtga
     3061 ccttctacct ggaatcgctt gtccttcatg gtggcgcaca cttttgcgac gtcagagaca
     3121 gcggccttac ctttatctga gagctctgca gagccggttg agaacagtac atccgcggac
     3181 agcacgacca cgactcttcc gtctacaagc ttcagcttca gatttccagc gtccgcgaga
     3241 gctttgaact gagtgcgaga tacttcaatg tccgcacttc gcgctctggc ctttgtttcc
     3301 gtttcctgca ctttctttgc gagagccgct ttttcctctg aaagcctg
//
LOCUS       DAOQJL010000442         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18455_length_3344_cov_4.494071, whole genome shotgun sequence.
ACCESSION   DAOQJL010000442 DAOQJL010000000
VERSION     DAOQJL010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18455_length_3344_cov_4.494071"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLG78_15850"
     CDS             complement(<1..590)
                     /locus_tag="PLG78_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNE24646.1"
                     /translation="MSALREFFVNLKKGTEHLNYGREILRDWGAEHALKLASRDQPIS
                     VYDLGCGHGTDIVNIRQHASIQTTAAFRWQLAGIENFSEYQKECKSLGIATHAVDLER
                     DTYPGKNASVDILIANQVLEHTKEIFWIFSEAVRLVRPGGKFLVGVPNLASLHNRLLL
                     LFGEQPTAQQSLSAHVRGFTKPDFKKFAETGGFFKLT"
     gene            633..1436
                     /locus_tag="PLG78_15855"
     CDS             633..1436
                     /locus_tag="PLG78_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE24647.1"
                     /translation="MNIKGKNVLITGASSGIGEAAARETARRGGIPLLVARREEELAR
                     VAREIQTESGIKAAFLVCDVSSPLDRIKIEPFARSAGGVSILINNAGITAHGRFDQTQ
                     PGIAEQAMNLNFFAALYTTQTLLPVMRETAGQKKIVLVSTPSALYGIPGRFAYSASKA
                     AGHAWMESIRIELKREGFDTLIFCPGYTRTQLRTRGLSADGTVLSEEQASGAKDPSQV
                     ATILLEAVRKDKRIAFTNSAGPLVYYLRTLAPGFLEKMLAKKLKKDFQT"
     gene            1463..2728
                     /locus_tag="PLG78_15860"
     CDS             1463..2728
                     /locus_tag="PLG78_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE24648.1"
                     /translation="MQPFIPSAFNLPVAWAVDFSLPILLKQLQNLDGVVLKPQDVKML
                     RSIRDDRLLYFSNHPTTSEPPVAYYVANVMGARFQFMAARQVFEWGGGLVGKFISSIG
                     AFSVLAGAADRESIKMSRAILSQPRGKLVLYPEGEPTSGENDNLLPFQQGAAQLGIWA
                     LEDARKSDPSADVTVLPTFVKYVMTGTEAHLRADLHQNIKIVEAKLGVDPGNRNLLRR
                     FLAVGRVMLEKAEKDYGVPMASEKDYDYRVGRVRHVILDNVAERLGLTSYDKKSDAIV
                     KLRHLLAVIEMILVKFPDPRLPNPTASDIEWAQRECQKAYDFIVIKPEYLLSRPSAER
                     FYEWLARFETTIDGSTNPRPRKAHVFFAKPFLLSEYYADYKKEKKVTVEKLTARLRAD
                     TQALLDQAVNLTEPLVRPYDIGAPEKPGP"
     gene            2725..>3344
                     /locus_tag="PLG78_15865"
     CDS             2725..>3344
                     /locus_tag="PLG78_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24649.1"
                     /translation="MRLDPRDPIFLQSPLDLIHKYYIENAIQDYRIYTDRLTNQFCAA
                     APAPVLLSNRARVKDQSFFLRNAEIRARKLTLIAAIDGEEREISGESFGDFLREGENR
                     IAIAQDDLLLQEEYPRQAGLFSYRPDRAKMDIQYRPDFGHGEHVQYFIASTGTPFGVF
                     SPGSFVFIDGVNRHRSQMIYDELLQSKIISRGGMVLDAPRLAGYDFL"
BASE COUNT          879 a          883 c          813 g          769 t
ORIGIN      
        1 gtgagcttaa agaatccgcc cgtctccgca aattttttga aatcaggttt cgtaaatccg
       61 cgaacgtgcg cgctcagact ttgctgggcg gttggttgct ccccgaacag aagcaacaaa
      121 cgattgtgga gactcgcaag attgggaacg cctactagaa attttccccc tggacgaacc
      181 agacgaactg cttctgaaaa gatccagaag atttcctttg tatgctccag cacttgattg
      241 gcaataagta tgtccacaga cgcattttta ccaggatatg tgtccctttc aagatctact
      301 gcatgcgtcg cgatccccaa tgatttgcat tctttctgat actcactgaa gttttcaatc
      361 cctgccagct gccagcgaaa tgcggcagtt gtttgaatgc tcgcatgctg gcgaatgttc
      421 acaatgtccg ttccgtgtcc acaacccaga tcgtacacgc tgattggctg atcacgagaa
      481 gccagtttca gtgcatgctc cgcgccccaa tcgcgcagaa tttctcggcc atagttgagg
      541 tgctccgttc ctttttttag atttacaaag aattcgcgaa gggccgacat atctccgcat
      601 actgtgccgg ggtctacggt caaattgaat ccatgaacat caaagggaaa aacgtactga
      661 tcactggagc ctcgagcgga atcggagaag ctgctgccag ggaaacagcc agacggggcg
      721 gaatccccct actggttgca agacgcgaag aagagctggc aagagtggcg cgcgaaatcc
      781 aaaccgagag cggaatcaaa gcagcgttcc tcgtatgcga tgtttcttct cctctcgaca
      841 gaatcaagat cgaaccgttc gcacgatctg ccggaggtgt atctatcctg atcaacaatg
      901 ctgggatcac cgcgcatggt agattcgatc aaacacaacc gggcattgca gaacaagcaa
      961 tgaacctcaa cttttttgcc gcactttaca ctacacagac gctactaccg gtgatgagag
     1021 aaactgcggg tcagaagaaa atcgttctgg tgtccacacc ctccgccctc tacgggattc
     1081 cgggcagatt cgcctacagc gcaagcaagg cggcaggcca cgcctggatg gaaagcattc
     1141 gcattgaatt gaaacgcgaa ggctttgaca ctctcatttt ttgtccaggt tacactcgca
     1201 cacaacttcg cacgcgtggg cttagcgcag acggaactgt actctctgag gaacaggcgt
     1261 cgggagcaaa ggatccttct caggtagcca ccatccttct tgaagcagta cgcaaagaca
     1321 aacgcatcgc tttcacaaac agtgccggtc cactggtgta ctatctgcgc acgctggccc
     1381 ccggattcct cgaaaagatg ctggcaaaaa aactaaaaaa agactttcaa acttagtgta
     1441 cgccggaaga ttttctctgt agatgcaacc attcataccg tcggctttca atctccctgt
     1501 tgcctgggcc gtagacttta gccttcccat tttactcaag caattgcaga atctagatgg
     1561 tgtggtcctc aagccgcagg atgtgaagat gctgcgctcg attcgcgatg atcgtctgct
     1621 ctatttcagc aatcacccga ctacttctga accgccggtt gcgtactacg tggccaatgt
     1681 aatgggagct cgctttcaat tcatggccgc ccgccaggta tttgaatggg gcggtggtct
     1741 tgtggggaag ttcatttcca gcattggagc attctcagta ctcgcaggcg cggcagatcg
     1801 cgagtccatc aagatgtcac gcgcaatcct cagccagccg cgcggaaaac tggttctata
     1861 tccggaggga gaacccacaa gcggggaaaa tgacaatcta cttccgttcc agcagggtgc
     1921 cgcacagctc ggtatctggg cactggaaga cgcgcgaaaa tcagatccat ccgcggatgt
     1981 tacagtcctc cccacgtttg taaaatacgt aatgaccgga acggaagctc atctccgagc
     2041 agatcttcat cagaacatca agatcgtaga agcaaaactg ggagtggatc caggaaacag
     2101 aaatttactg cggcgattcc tcgctgtggg tcgcgtgatg cttgaaaaag cggaaaaaga
     2161 ttacggcgtc cctatggcat cagagaaaga ctacgactac cgcgtggggc gtgttcgcca
     2221 tgtaatcctg gacaacgttg cagagagact cggcctcacc tcttatgata aaaagagtga
     2281 cgcgattgtc aaactgcgcc atcttctcgc agtcatcgaa atgattctgg tcaaatttcc
     2341 agacccacgc ctgcccaatc ccaccgcgag cgatatcgaa tgggcacagc gcgaatgcca
     2401 gaaagcatat gacttcattg taatcaaacc cgagtatctg ctttcgcgac cttccgcaga
     2461 gagattctac gaatggctgg cacgattcga aacaaccatt gacggatcca caaatccgcg
     2521 acccagaaag gctcatgtat tcttcgcaaa gccctttctt ctatctgaat actatgcgga
     2581 ctacaagaaa gaaaagaagg ttaccgtgga gaaactgact gcccggcttc gtgcggacac
     2641 gcaggcactc ctggatcagg ccgtcaatct gactgagccc ctggtacgtc cgtacgacat
     2701 tggcgcgcca gaaaaaccag gcccatgaga ctggatccgc gagatccaat cttcttacag
     2761 tcgcctctgg atctaattca taagtattac atagaaaatg cgattcagga ctatcgcatt
     2821 tatacagaca gactcacaaa ccaattctgt gcagccgctc ccgcaccggt tttactatcc
     2881 aatcgtgccc gcgtcaaaga ccaatctttc ttccttcgca acgcagaaat ccgggcacgc
     2941 aagttgaccc tcatcgcggc gattgatgga gaagaaagag aaatatccgg agaatcgttc
     3001 ggtgattttt tgcgcgaagg ggaaaacaga atcgccatcg cccaggatga tcttctactg
     3061 caagaagagt atccacgcca ggcaggcctg ttcagctaca ggccagatcg ggcaaagatg
     3121 gatatacagt atcgtcccga tttcggtcac ggagaacacg ttcaatattt cattgcttct
     3181 actggcacgc cgttcggagt ttttagtcca ggcagctttg tgtttataga cggagtcaat
     3241 cgtcatagat ctcagatgat ttatgatgaa ttgctccaat ctaaaatcat aagccgtgga
     3301 gggatggtcc tggacgcgcc aagacttgca ggatacgact ttct
//
LOCUS       DAOQJL010000443         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18489_length_3341_cov_3.947961, whole genome shotgun sequence.
ACCESSION   DAOQJL010000443 DAOQJL010000000
VERSION     DAOQJL010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18489_length_3341_cov_3.947961"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLG78_15870"
     CDS             complement(<1..483)
                     /locus_tag="PLG78_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNE24650.1"
                     /translation="MSKPFLIAIDQGTTGSRVFCFDTAGKVLSTAYREFTQHFPKPGW
                     VEHDAMEIWTGVCSLLAEALSKGGLDAKNAAGIGITNQRETCVVWDRKTGEPIHRAIV
                     WQCRRTADFCEELKKRGLADEVRRKTGLVIDAYFSGTKIRWFLENVAGVRARAEKGEL
                     M"
     gene            742..1113
                     /locus_tag="PLG78_15875"
     CDS             742..1113
                     /locus_tag="PLG78_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNE24651.1"
                     /translation="MEEQQMQQAAAGGMAGNLIGLVIAVIYIVGLYKTFEKAGQPGWA
                     AIIPLYNMIVLLKVAGKPMWWILLLLCTGPIGMILVGMAVAERFGKSSVFGIVALGLF
                     GFVGYPMLGFSDATYTAPPAA"
     gene            complement(1154..1429)
                     /locus_tag="PLG78_15880"
     CDS             complement(1154..1429)
                     /locus_tag="PLG78_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24652.1"
                     /translation="MVSESGLGSHSNTRIVFRLAALGFFNIVIILESNPQDSDVPKYR
                     ASLIAVSAWLAGRVEFRAACAAIHPPKWRAAQMISLAAVFVKEKSRE"
     gene            complement(1520..2497)
                     /locus_tag="PLG78_15885"
     CDS             complement(1520..2497)
                     /locus_tag="PLG78_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE24653.1"
                     /translation="MKKAIVLAIVLLSACKQGAWVSVDGSRLTESDVEKEMPDQYKRL
                     KEEYNSQMRDLLEQVAVKKMMQKAAKEKGVDEETYARQISEKAPEPTEKQIADFYEQI
                     KKTGQTDGQSLAQLHSRIAMHLKQEGGREAMQNEISELKKKYNYTYDFSRTKVETAGN
                     PERGNASAKVTVVEFSDFECPYCIRVQPTLQKLREKYNGKIRWVFKDFPLNFHPHAMD
                     MHIGSKCVYRVNKDHYWEFFDKMFAQDRSADILTKEGTAKLAASLGTDMNKYKACTED
                     PAVAAEIKDSIKQGEAVGVSGTPAFFINGRMLSGAQPMAAFEEVIQQEL"
     gene            complement(2521..2838)
                     /locus_tag="PLG78_15890"
     CDS             complement(2521..2838)
                     /locus_tag="PLG78_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE24654.1"
                     /translation="MQDIDTVLGDDIQFRGRLQFKRNLKINGAFRGKIQTGGQLIVGS
                     GAKVEADVEASHITVEGELRGNLQASKSLEIKRNAQVSGDIRTPDLHVESGSRFTGSC
                     IMD"
     gene            2917..>3341
                     /locus_tag="PLG78_15895"
     CDS             2917..>3341
                     /locus_tag="PLG78_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24655.1"
                     /translation="MMELREQSLAPLWKVKPSRREGYHKTYYTETAEGDSILIDFNNV
                     RQMVRIQVTIESEGGRQYACVIKSGTILQERDLTVGRPMDITSRIAPLRFFIGSIPDD
                     MLRSAIRGSYGIPLNGPSPGMRRARIVHTRKVPRMQLLL"
BASE COUNT          722 a          863 c          833 g          923 t
ORIGIN      
        1 catcagttcg cctttctcag cgcgagcgcg cactcctgct acgttttcca ggaaccagcg
       61 aatctttgtg ccggaaaaat atgcatcgat gaccagtccg gttttcctgc gaacttcgtc
      121 tgccagtccg cgctttttga gttcttcgca gaagtcagcc gtgcgtctgc attgccacac
      181 aatggcacga tgaattggct ctccagtttt gcggtcccac accacgcagg tctccctctg
      241 gtttgtaatg ccaatgcctg ctgcattctt tgcgtccagt ccgcctttgc tcagagcttc
      301 tgccagaaga gagcacactc ccgtccagat ttccatggca tcatgttcga cccaccctgg
      361 tttcgggaag tgctgggtga attcacggta agcagtagag agaactttgc ccgctgtatc
      421 aaagcagaat actctggagc ctgtagttcc ctggtcgatg gcaatgagaa atggtttgga
      481 catgtgcgat tcgccctggt gataggaaga tgtgcaagcc gaaaatgccg ggatgtgctt
      541 ttcggcaaca gcctgtgctt tcttttcagg caggctccgg cgtccgggac agctcgagct
      601 ccagacaggc aaaagatcca aaggaaacgt ggccaaatcg tgagtgatcc aaaattttcg
      661 tttgaccttc ttcgcggtga ttctcaggtt actggcagat tcgcgtgcga aagccgtgaa
      721 aaaacatagg agtgttcaaa aatggaagaa caacaaatgc agcaagctgc cgccggggga
      781 atggctggta acttgattgg acttgttatc gcggtaatct atattgttgg cctgtacaag
      841 accttcgaaa aagcaggtca gccaggctgg gcagcgatta tccctctcta caacatgatc
      901 gtgcttctga aagtagctgg aaagccaatg tggtggatcc tgcttctcct ctgcacgggc
      961 ccaattggaa tgattctggt tggtatggct gtagcagaac gcttcggcaa atcttcagtc
     1021 ttcggcattg ttgccctggg tctgttcggt tttgtcggtt atccaatgct tggattcagc
     1081 gacgcaactt acactgctcc accagcagcg taactctcaa gcatcccgcg cccacggcgc
     1141 gggatcgcat tttctattct cttgatttct ccttaacaaa gaccgccgcc aggctgatca
     1201 tctgcgccgc cctccacttt ggcggatgga ttgccgcgca ggctgcgcga aattctacgc
     1261 ggccggcgag ccaggcagac acagcaatca gacttgctcg atactttgga acgtcggagt
     1321 cttgtggatt ggactccaga atgattacga tattgaagaa gcccagcgca gcaaggcgaa
     1381 agactatgcg cgtattacta tgagagccta aaccagattc cgaaaccatt ccttccgtgc
     1441 gggaaagatc gattccgtaa cgttatgcga cgaagtacct gctgcttttt tccgtcgctt
     1501 ttcgttgtcc cggcagttct taaagttcct gctggatcac ttcttcaaat gcagccatag
     1561 gctgggctcc ggaaagcatt ctaccattga tgaagaacgc tggcgttcca gatactccca
     1621 ctgcttctcc ttgcttgatg ctgtctttga tttcagcggc aactgcagga tcttctgtgc
     1681 aggctttgta tttgttcatg tctgttccaa gagaagccgc gagctttgct gttccttctt
     1741 ttgtgagaat gtccgcagag cggtcctgag cgaacatctt gtcaaagaat tcccagtaat
     1801 ggtctttgtt cacgcgatac acgcatttgg aaccaatgtg catgtccatc gcatgaggat
     1861 ggaagttcag agggaaatct ttaaacaccc agcggatttt gccattgtat ttttcgcgca
     1921 gtttctggag ggtaggctga acgcgaatgc aataaggaca ttcgaaatca gagaactcaa
     1981 ccacagtgac tttcgcagaa gcattgccgc gttcaggatt tccggcggtt tccactttgg
     2041 tgcgtgagaa atcgtatgta tagttatact tcttttttag ctcagagatt tcgttctgca
     2101 ttgcttcgcg cccaccttct tgcttgaggt gcattgcgat tctgctgtga agctgcgcca
     2161 ggctctggcc gtcagtttgt cctgttttct tgatttgttc gtagaaatca gcgatttgtt
     2221 tttctgttgg ctctggagct ttttctgaaa tctggcgagc gtaggtttct tcatctactc
     2281 ccttttcttt tgcagccttc tgcatcatct ttttgactgc aacctgctcc agcagatcac
     2341 gcatctgaga gttgtattct tctttcagtc ttttgtattg atcaggcatt tccttttcaa
     2401 cgtctgattc agtgagtctg cttccatcta cggaaaccca ggcaccctgt ttacacgcgg
     2461 acaacagtac gatcgcgagc acgattgctt ttttcatttc attggtcctc tgaggtgtga
     2521 tcaatccatg atgcagcttc cggtgaaccg ggagccggat tctacatgca ggtctggagt
     2581 gcgaatgtca ccggatactt gcgcgtttct tttgatctca aggcttttag aagcctggag
     2641 attgccgcga agctctcctt ctactgtgat gtgacttgct tctacgtctg cttctacctt
     2701 cgcgccagat cccacgatga gctggcctcc ggtttgaatt ttgccgcgaa acgcgccgtt
     2761 gatcttcagg ttgcgtttga attgcagccg tcctcggaac tggatatcat ctccgagaac
     2821 tgtgtcgata tcctgcatac tctctggaat gggcgagggc ctccagggtc aagaaaaatg
     2881 cacgagtact caggctgaat tgccgtaaat tcgaatgtga tggaacttcg cgaacagtcc
     2941 ctggctcccc tgtggaaagt gaaaccttct cgcagagaag gatatcacaa gacttactac
     3001 acagagaccg cagaaggcga ttccattctc attgacttta ataatgtccg ccagatggtc
     3061 cgcatccagg taaccatcga atcagaaggc ggccgccagt acgcctgcgt cattaaatcc
     3121 ggcaccattc tccaggagcg cgatttgact gtgggtcgtc ccatggacat caccagcaga
     3181 attgcgccgt tgcgcttctt cattggatcg attccagacg acatgcttcg ctccgccatt
     3241 cgtggcagct atggcatccc actgaatggc ccgagtcccg ggatgcgtcg cgctcgtata
     3301 gtgcacacgc gaaaggttcc gagaatgcag cttcttctca g
//
LOCUS       DAOQJL010000444         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18634_length_3325_cov_3.034862, whole genome shotgun sequence.
ACCESSION   DAOQJL010000444 DAOQJL010000000
VERSION     DAOQJL010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18634_length_3325_cov_3.034862"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLG78_15900"
     CDS             complement(<1..558)
                     /locus_tag="PLG78_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24656.1"
                     /translation="MAFLRDWPVKLGALVVAGILALYVEFAQNVTRVQHVRVTVPPIP
                     PGLVFASRVPSFMDVELYGPSELMDVAASDFKMQLINRRPVRGENIFRVKMSPELPSG
                     LEASYPKELKVMLDEVLYRELPVEAAYDHSMPLGGIRIRPRTVVVVGPYQKMAEMDRV
                     MTLPVTLSGSGSRSIRAVLADLPDFM"
     gene            complement(588..1394)
                     /gene="cdaA"
                     /locus_tag="PLG78_15905"
     CDS             complement(588..1394)
                     /gene="cdaA"
                     /locus_tag="PLG78_15905"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNE24657.1"
                     /translation="MLENLWNSVRPFVRFIDILIVAFLLYRAYILLSRTRAMQLLLGF
                     GLILLLDVIARRFSMDTLSWLITNVSQYLVFGLIVLLQPELRRLFAEIGKMPLFQWVN
                     PPLSVPLDEIIAAASSMARSRTGSIIVLMKEIRPHGILENSVRVEGNISRELLETIFH
                     KDTPLHDGAVIIEGAKILAASCYLPLSSSRVLKKTLGARHRAALGMSEESDAVILVTS
                     EESGKISVMQNGEVLTPVRAGELKSLLSELLGKTPRSIAEIAEEKKAQEA"
     gene            complement(1387..2130)
                     /gene="dapB"
                     /locus_tag="PLG78_15910"
     CDS             complement(1387..2130)
                     /gene="dapB"
                     /locus_tag="PLG78_15910"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNE24658.1"
                     /translation="MIRFVMSGARGRMGQMILNTATLPDFKDRITASGQIDVGDDPVS
                     QIQGASVMIDFSSPANTLAMAKLCMAHNVGFIVGTTGLTSAQETEIESASSKIPVLIS
                     PNMSLGVNLLFHLTGIAVSAVPQADVEIVELHHNQKVDAPSGTAARMKDIVKEKRNLA
                     EKDVVHGREGLVGKRKQEEIGMHAVRGGDTPGDHTVYLFLDGERLEITHRALSRIVFA
                     RGALQAALFLSGKPAGIYTMNDVLGIANA"
     gene            complement(2127..3011)
                     /gene="dapA"
                     /locus_tag="PLG78_15915"
     CDS             complement(2127..3011)
                     /gene="dapA"
                     /locus_tag="PLG78_15915"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNE24659.1"
                     /translation="MEFRGLYTAVVTPFTSGGSQIDYDAYAKLLERQIAGGVAGVVPC
                     GTTGESPTLTHEEHSELIRRTVSLVKKRVQVIAGTGSNSTAEAIELTKAACSDGVDAV
                     MIVNPYYNKPTQEGLFRHFEAVAGVSSVPVMVYNIRGRTAVNIEPETFTRLKSVARIA
                     CVKEASGDLGQMARIHQLAGDRIALLSGDDNLTPAVMAIGGLGVVSVASNIFPKRLSA
                     MVKHYLAGDYKSGNAIFYDLLEFMQALFWETNPIPVKAGLAALGLCKPDIRLPMTPMS
                     EGKRREFEILLNALGEDR"
     gene            3069..>3325
                     /locus_tag="PLG78_15920"
     CDS             3069..>3325
                     /locus_tag="PLG78_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24660.1"
                     /translation="MSGTATPVIAFLCFVSSFVAAGLLTRIQLLPDRPNPRSMHTRVT
                     PRSGGLSLQVPFLMAIAYFAWRDGWSAHVLAVLVGSTAFSLL"
BASE COUNT          811 a          898 c          826 g          790 t
ORIGIN      
        1 cataaaatca ggcagatccg cgagaactgc gcgaatagaa cgagaaccgg aaccgctgag
       61 agtcacaggc aaagtcataa cgcggtccat ctcggccatc ttctgatacg ggccaacaac
      121 aactacagtg cgtggtcgaa ttcgaatccc gccaagaggc atggagtggt cgtaggctgc
      181 ttctacgggt agctcacgat acaacacttc atcgagcatt actttcaatt ctttaggata
      241 cgaagcctcg agtcctgatg gtagctcggg ggacatcttc acgcgaaaga tgttttcccc
      301 tctcacaggc ctgcgattga tgagttgcat tttgaaatca gacgccgcca catccatcaa
      361 ctcggacgga ccatacagtt caacgtccat gaaagaagga accctggaag caaataccag
      421 cccgggcgga atgggtggaa cggtaacgcg cacatgctgc acccgagtga cgttctgcgc
      481 aaactcaaca taaagagcca gaattcctgc aaccacaagt gcgccaagct tcactggcca
      541 gtcgcgaaga aaggccatgg ccctcttcca tagttgacga aatccgatca agcttcctgc
      601 gccttcttct cttccgcaat ttcagcgatg gatcgaggag ttttcccaag aagttcagac
      661 aagagagatt tgagctcacc cgcacgcaca ggtgtgagga cttctccatt ttgcatgacg
      721 cttatttttc ctgattcctc agaagtaacc aggatgaccg catccgattc ttccgacatg
      781 cccagggctg cgcgatggcg cgcgccaagc gttttcttca aaacgcgcga gcttgataga
      841 ggaaggtaac aggacgcagc cagaatcttc gcaccttcta tgataacggc cccatcatga
      901 agcggagtat ccttgtgaaa gatggtttca aggagttcgc gtgaaatgtt tccttctact
      961 ctgacagagt tttcaagaat gccatgagga cgaatctctt tcataaggac gatgatcgag
     1021 cccgtcctgc ttcgagccat actcgatgca gccgcaatga tttcatcgag tggaacgctg
     1081 agtggtggat ttacccactg gaacagcggc attttgccga tttcagcaaa caacctgcga
     1141 agctccggct gaaggagcac aatcaaacca aatacgagat actgcgaaac gtttgtgatc
     1201 aaccaggaca gagtgtccat ggagaatcta cgcgcgatga catccagaag caggatgagt
     1261 ccaaagccaa gcagaagctg catggcgcgc gtgcgtgaca gaaggatgta agcgcgatat
     1321 aaaaggaatg caactatcag tatgtcgata aaacgaacaa acggacgcac cgaattccag
     1381 agattctcaa gcattcgcaa tccccagaac atcgttcatt gtgtagattc ctgcaggctt
     1441 tccggacagg aagagcgccg cctggagtgc gccgcgcgca aatacaatgc gtgatagggc
     1501 gcgatgagta atctccagtc tttctccgtc taaaaacaaa tacacagtat gatcgccggg
     1561 tgtatcaccg ccgcgcactg cgtgcatacc aatttcttcc tgcttgcgct tgcctacgag
     1621 cccttctctt ccgtgtacca cgtccttttc tgcgagattt ctcttctctt tgacaatgtc
     1681 cttcatgcgc gccgctgtgc cggaaggcgc gtccactttt tgattgtgat ggagctctac
     1741 aatttccacg tctgcttgcg gaacagctga gactgcgatt cctgtaagat gaaaaagcaa
     1801 attcactccc agtgacatgt ttggactgat gagcactgga attttagaag atgcagactc
     1861 aatttctgtc tcttgcgcgg aagtgagtcc ggtagtgcct acgataaagc caacgttatg
     1921 cgccatgcac aactttgcca tggcaagggt atttgcggga cttgaaaaat caatcatgac
     1981 gcttgcaccc tggatctgag aaacagggtc atctccaacg tcaatttgtc cggatgccgt
     2041 tatgcgatct ttgaaatcag gcaacgtggc cgtattcaag atcatctggc ccattctacc
     2101 acgggcgccc gacatcacaa aacgaatcat ctatcttctc ccagagcatt cagcagtatt
     2161 tcgaattcgc gacgtttgcc ttcgctcatc ggagtcatgg ggaggcggat gtcaggcttg
     2221 cacagaccca gggccgccag acctgctttt actggaattg gattggtttc ccagaaaaga
     2281 gcctgcatga actcgagaag gtcatagaag atcgcattgc cagacttata atcacctgca
     2341 aggtaatgct tgaccatcgc agagagtctc tttggaaaaa tattggatgc gacggataca
     2401 actccaaggc cgcctatcgc catcaccgca ggcgttagat tgtcatcccc cgaaagaagt
     2461 gcgattctat ctcctgccag ctgatggatg cgagccatct gtccaagatc tccgcttgct
     2521 tcttttacgc aggcgatgcg cgcaacgctc ttgagtcgtg tgaatgtctc tggctcaata
     2581 ttgactgccg ttctgccgcg aatgttgtag accatcaccg ggacactcga tacgcctgcc
     2641 actgcttcaa aatggcgaaa gagaccttcc tgagttggct tgttgtagta cggattgaca
     2701 atcatcaccg catctactcc atccgagcag gcagctttcg ttagctcgat ggcctctgca
     2761 gtggaatttg acccggtgcc tgcgatgacc tgcacccttt ttttcacaag agagactgtg
     2821 cggcggatga gttcactgtg ttcctcatgg gtgagcgtag gagattcgcc cgtggtcccg
     2881 caggggacca ctcctgccac gcctccagct atctgacgct ccagaagctt cgcgtacgca
     2941 tcatagtcga tctgactgcc cccggatgtg aaaggcgtga cgactgccgt atagagaccc
     3001 ctgaattcca tcttcgacct ggcctaaata ttatttcaag aaaagcggct tctctttttt
     3061 gtgcaaacat gtctggaacg gccactccgg tcatcgcttt tctgtgtttt gtatccagct
     3121 ttgttgcggc cggactgctc acacgcattc aacttctgcc ggatcgcccc aatccgcgct
     3181 ccatgcacac gcgggtcaca ccgagaagtg gcgggctcag cttgcaggtg ccttttctca
     3241 tggcgatcgc atactttgcg tggcgagacg gatggagcgc gcacgttctg gcggtgctcg
     3301 tgggatccac cgcattctct cttct
//
LOCUS       DAOQJL010000445         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18640_length_3324_cov_4.744570, whole genome shotgun sequence.
ACCESSION   DAOQJL010000445 DAOQJL010000000
VERSION     DAOQJL010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18640_length_3324_cov_4.744570"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLG78_15925"
     CDS             complement(<1..1125)
                     /locus_tag="PLG78_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE24661.1"
                     /translation="MIDPSPGFSPNPDGKPNYTVDGKPLHSRNNPEEEARRFIAGILS
                     QNPTGNIIIAGVGAGYVFEILRHLPAERVFFLEPIGAIRERLLGVAPPGSRILASIED
                     LPSGPAQIFPLPGHRARFPIAHAAINDYAKSLSREPTETRIDRATTARFFRRWSRNFF
                     RSLFYDRLEFISQPVKGSGTILFCGAGPTLFDDLEQLDISRATLCAADTALAPMLRRG
                     IIPHYVISVDSGYGTLYHLKAAAHAGLEQHPEITAVSWSGGHPALSLFFRRRIHFLTT
                     FPLDQILSQGILNNLPVFENESRNTAGIAVRLAWSTGSHLAFAGTHFRSQAGLSHIPG
                     TGYDLFASESQSRTRPISQYRPGGYSSDLTTRNRSSLEGVK"
     gene            complement(1118..1612)
                     /locus_tag="PLG78_15930"
     CDS             complement(1118..1612)
                     /locus_tag="PLG78_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNE24662.1"
                     /translation="MSRDAKEFEISKLLQLAEKEKYATTVAAFEVIDKLEQIDIPKKY
                     TNRKPAVKAMIALSDGIVQYDYIADEARQALLEELHGPGATSASPTAEIAAARGRVPV
                     VEESEEDLGDEDIGEIEPTAKLDEDSEDGDESEAADEEEEEDEDEEDSDDDDDEEDSE
                     PEDD"
     gene            1655..2233
                     /locus_tag="PLG78_15935"
     CDS             1655..2233
                     /locus_tag="PLG78_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE24663.1"
                     /translation="MPLKKTLFLAWVLLAVFACRPAARSNFGVDQWSGRLLDGSVIHF
                     SQIDAPKLVLNFYSPTCQPCIQEIPALEHFAAEAAKRKIPVYVALESSPSSHGLNLPA
                     DATPEDRFQAIRNRMMEDIQKYSMRLPVVVMDDNFRINPEYGTVTGTPETLIFSTKPL
                     LLRYNFIGPIGTAQDAATIERQPRYQFALERL"
     gene            2233..3120
                     /locus_tag="PLG78_15940"
     CDS             2233..3120
                     /locus_tag="PLG78_15940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE24664.1"
                     /translation="MARLQKKSARSPSRSEKRAGSSVVLLSASIITFNEEHRIFDCIA
                     SVHDFCDEVLVLDSFSTDKTETIARKFPKVRFYKHAFDGHVQQKNRAFEMCRGKWILS
                     LDADERCDPELASSIQEFLRTSADHGPDGMRINRLTIHMGRPIHHGGWYNARYRLIKK
                     GKAAWGGENPHDQIFLHDEPRWKTNLGPVLSGNLVHYSFKDLSDQIGTINKFSSIIAF
                     TRKGKGKRFSLIRLLWKPFQKFLEIYLLKRGFLDGMPGFVIAYSSAFSYFLREAKLYE
                     LHHGLDRPSNLRPDYKVQR"
     gene            3138..>3324
                     /locus_tag="PLG78_15945"
     CDS             3138..>3324
                     /locus_tag="PLG78_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNE24665.1"
                     /translation="MRIKVRVKPGSKKPGIEKTEDGWIIRVRERAVENKANEAVIEAA
                     AQELGIPRSRLRIVLGAT"
BASE COUNT          775 a          833 c          869 g          847 t
ORIGIN      
        1 ctttacacct tcgagactac tgcggttgcg cgtcgtaaga tcgcttgagt atccgcctgg
       61 cctatactga ctgatcggcc ttgttctgct ttgcgattcg gacgcgaata gatcatatcc
      121 ggttccgggt atgtgcgaaa gaccggcctg actccgaaaa tgagtgcctg caaaagccag
      181 atggctcccg gtagaccacg ccagccgcac agcaattcca gcagtgtttc tgctttcgtt
      241 ttcaaacaca ggcaggttgt tcaggattcc ctggctcaag atctggtcga gagggaacgt
      301 agttaagaaa tgaatgcgtc tgcggaagaa gagtgagaga gccgggtgac cgccagacca
      361 gctcactgcg gtgatttcag gatgttgctc cagcccagcg tgcgctgctg ccttcaaatg
      421 ataaagagtc ccgtatccgc tatcgactga aatgacatag tgcgggatga tcccgcggcg
      481 aagcatggga gccagggcag tgtccgccgc gcacagggtt gctctggaaa tgtccagctg
      541 ctctagatcg tcgaataacg ttggccccgc gccgcagaaa agaatggtgc ccgaaccctt
      601 tacgggctgc gaaatgaact caaggcgatc gtaaaaaaga ctgcgaaaga aattgcggct
      661 ccacctgcga aagaagcgag cggttgtggc acggtcgatt cgggtctccg ttggttcgcg
      721 cgaaagactt tttgcgtaat cgtttatggc tgcatgagca attggaaatc gagcacgatg
      781 tcctggaaga ggaaagattt gtgctggacc agatggcaga tcctctatgg aggcaaggat
      841 gcgtgagccg ggcggtgcca ctcccagcag gcgctctcga atggcgccta tcggttcgag
      901 aaaaaagacg cgttctgccg gtaggtggcg aaggatttca aatacatagc cggctccgac
      961 gcctgctatg atgatgttgc ctgttggatt ctgcgaaagg atgccggcga tgaatcgccg
     1021 ggcttcttct tctggattgt ttctggagtg aagcggttta ccgtctacag tgtagtttgg
     1081 ttttccgtct ggattcggag aaaagcctgg ggagggatca atcatcctcc ggctctgaat
     1141 cctcctcgtc gtcatcgtcg tcagagtcct cttcgtcctc gtcttcctct tcttcttcgt
     1201 cagccgcttc ggattcgtct ccgtcttcgc tgtcttcatc gagcttcgcg gttggttcga
     1261 tttcgccaat gtcctcatcg ccgagatctt cttccgattc ttctacaaca ggaacccttc
     1321 cacgcgctgc tgcaatttct gcagtaggag acgcagaagt tgcgcccggg ccatggagtt
     1381 cttcgaggag agcctgacgg gcctcatctg cgatgtagtc gtactgaaca attccgtctg
     1441 acagtgcaat cattgccttt acagcaggct tgcgatttgt atatttcttg gggatgtcaa
     1501 tctgttccag cttgtcgatg acttcgaaag ccgctacagt tgtggcatac ttctctttct
     1561 ctgcgagctg gagaagtttt gatatttcaa attctttggc gtctctggac atggtaattt
     1621 taaggaaatg ccactttttc aatttggtac aattatgcca ctaaaaaaaa cactgttcct
     1681 ggcctgggtt ttgctagcag tcttcgcctg caggcctgct gcgaggtcca atttcggcgt
     1741 agatcagtgg tccggccggc tgctggacgg ctctgtgatt catttcagtc agatcgacgc
     1801 cccgaagctg gtgctcaatt tttatagccc cacctgccag ccgtgcattc aggaaatccc
     1861 agcgctcgag cactttgccg cggaagcggc taaaagaaag attccggtgt atgtagctct
     1921 tgaatccagt ccttcttcgc acggtctcaa tctaccggcg gacgcaaccc cggaggatag
     1981 attccaggca attcgcaatc gcatgatgga agacattcaa aagtattcga tgcgccttcc
     2041 agtggtagtc atggacgata actttcgaat caatccagaa tatggaactg tgactggaac
     2101 tcctgagact ctgatcttct ccacgaaacc gctcttgctc cgatataatt tcattggtcc
     2161 catcggcaca gctcaagatg cggcgacgat tgaaagacag ccacgctacc agtttgcact
     2221 ggagagactt tgatggcaag attgcagaag aaatcggcgc ggtctccatc gagatcagaa
     2281 aagcgagctg gttcatccgt ggttcttttg tctgcgtcca tcatcacctt caatgaagag
     2341 catcgcatct ttgactgtat tgcgtccgtg catgacttct gtgacgaagt gcttgtgctg
     2401 gactctttct caacggataa aacagaaacg attgctcgca agttccccaa agtacgtttc
     2461 tacaaacatg ctttcgatgg tcacgtgcag cagaagaatc gggcatttga aatgtgcaga
     2521 ggaaaatgga ttctttctct cgatgcagat gaaaggtgtg atccagaact tgcgtcctct
     2581 attcaggaat ttcttcgcac atccgcggat catggcccgg acggaatgcg aatcaatcgc
     2641 cttacgattc atatgggcag acccattcat catggtggtt ggtacaatgc gcgttaccga
     2701 ttgatcaaga aagggaaagc tgcctgggga ggagagaatc cgcacgatca gatcttcctt
     2761 cacgatgaac ccaggtggaa gacaaatctt ggtccggttc tttccggaaa cctggtacac
     2821 tattctttca aagatctttc ggatcaaatt ggcaccatca acaagttttc gagcatcatt
     2881 gccttcactc gaaagggaaa aggaaagaga ttctcactga ttcgactcct gtggaagcca
     2941 tttcagaaat tccttgagat ctacctgctc aagcgtggct tcctggacgg aatgcctgga
     3001 tttgtgatcg cgtattcttc cgccttttcc tacttcctga gggaagcaaa gctgtatgaa
     3061 cttcaccacg gtctcgatcg accatccaat cttcgtccag actataaagt acaacgttag
     3121 tcgcttttga ggtctgtttg cgaatcaaag tccgcgtaaa gcctggcagc aagaaacccg
     3181 ggatcgaaaa gacggaagac ggctggatca ttcgggtcag ggagcgcgct gtggaaaaca
     3241 aggcaaacga ggcagtcata gaagccgcag cacaagagct tggcattcct cgcagtcgtc
     3301 tccgaattgt gcttggtgca actt
//
LOCUS       DAOQJL010000446         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18749_length_3314_cov_3.962872, whole genome shotgun sequence.
ACCESSION   DAOQJL010000446 DAOQJL010000000
VERSION     DAOQJL010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18749_length_3314_cov_3.962872"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1237)
                     /locus_tag="PLG78_15950"
     CDS             complement(275..1237)
                     /locus_tag="PLG78_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE24666.1"
                     /translation="MASAFSARGECSIPEAETQRSIESAFYEVDASMSNWSQTSEVSR
                     FNSMPVGESMAMSQSLEIVLGKSVDVYEKTEGLFDPAIGELIEAWGFGVNEIPRAAWV
                     RSQARVDSGWNGVTRRGGRWIRSKPVTLNVSGIAKGYAVDLALSRTVSKCERLLIDLG
                     GEVGTKGSWTVAVRHPGDKNRTIGQVDVTDAAVATSGTYANSRTLAGVRFSHILHPRT
                     GEPVRALLSVTVVGPDGAYADALATALVLAFSMNDEDRTAGRILARFPAYSAIAVRPY
                     STHATGLSNLEEGVGSYAITIAGLSIPFKCTNPAASRNGLLLCL"
     gene            complement(1285..1755)
                     /locus_tag="PLG78_15955"
     CDS             complement(1285..1755)
                     /locus_tag="PLG78_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24667.1"
                     /translation="MYADFRIPLAAFSIFAGLVFVTGVILYAGKAGISTDENYEYYMG
                     SEAARRQQPDRVDRFLAAKTTAGILKTQTPHSIGFAILFFILFHLLRSLHPAKSTGWF
                     GLLFLLALLVDFAMPFCMLSGWSLFAALRFPFVCFFAACGIGGSAFLCVKVCWS"
     gene            complement(2152..2622)
                     /locus_tag="PLG78_15960"
     CDS             complement(2152..2622)
                     /locus_tag="PLG78_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNE24668.1"
                     /translation="MLLFSQTIETAEMILKKEFPGTAIERKTLYLTREEGEILSRCCG
                     ESSRVHTFYLASDQGGVRGFGIFDTHVVRTKEETLFIVVDAAGIVKHVQVAAFLEPRD
                     YLAPDRWFALFKGKTAGSSFDQPPITGATLTVKAAKNAVRRALELHRLHARSLQ"
     gene            complement(2705..>3314)
                     /locus_tag="PLG78_15965"
     CDS             complement(2705..>3314)
                     /locus_tag="PLG78_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24669.1"
                     /translation="TSARVPVQIAEAHYLVDKGAFTTRGVIARGWLSDAGTRALNKQA
                     NKNIAKEVEGGYLEGGVNVLHFFNSPKKLVLFVRGEYVNTQRRTIERYPGGKEDLDDL
                     ICANLGCLTTSQLTNGNRSLGIVAASDSGKELYGVRGVPDRTQDRRIYTMGMAFYPHE
                     NITLKLDYAMQFSKSDYHKDIEQLNPSNNKIDQVNFGLGFIF"
BASE COUNT          848 a          912 c          832 g          722 t
ORIGIN      
        1 ctaagaatct catctattat actcatttct tgcttcctcc cgccacttgc ggctgagaac
       61 ggcgaacgcc tgatccggag accgcgttgg tcgaatttac gcagaaatgc ggaaaacttc
      121 ttaggactca agatcaagga cagaggacct ggttttttct atccgacctc tgggccagat
      181 ttcgctttct gagagagaag tcatggttga gcgcgcaatg acatcgcgag gaacaaagaa
      241 caatcgtatt gtttctctta tactcgacct gcaatcaaag gcagaggagt aaaccgttcc
      301 gggaggcggc cgggtttgta catttaaatg ggatggacag gcctgcaatt gtgatcgcat
      361 aagaccccac accttcttcc agattcgaca gccccgtagc atgcgttgaa tatggacgca
      421 cggcgatggc ggagtacgcg gggaatcgag cgaggattct tcccgcagtc ctgtcctcgt
      481 cgttcattga aaacgcgagc acaagggccg ttgcaagggc gtctgcgtac gccccgtctg
      541 gccccacgac agtcaccgac aaaagcgcgc gcactggttc tcctgtgcgt ggatgaagaa
      601 tgtgtgaaaa ccgcacacct gcgagggttc tagaatttgc gtatgttcca cttgtggcga
      661 ccgctgcgtc cgttacatca acttgcccta tcgttctgtt cttgtccccc ggatgacgga
      721 ccgcaaccgt ccaagacccc tttgtgccga cctccccgcc gaggtcgatg agaagccgct
      781 cgcattttga cacggtgcgg gacagtgcca gatcgactgc gtaccctttt gcgatgccgg
      841 atacgtttag cgtaaccggc ttgctcctaa tccagcgtcc acctctacgg gttactccat
      901 tccaaccgga atctacgcgc gcctggctcc taacccaagc ggcacgcgga atttcgttca
      961 cgccgaatcc ccaagcctcg atcaattcgc cgattgcggg atcgaagaga ccttctgttt
     1021 tttcgtaaac gtccacagat ttccccagta cgatctctag agattgggac atcgccatcg
     1081 attccccaac cggcatggag ttgaaccggg atacctcgct ggtttgcgac caattcgaca
     1141 tcgatgcatc gacttcataa aatgcagatt caatgcttct ttgggtttct gcttccggga
     1201 tactgcattc gccgcgcgcg gagaatgcac tcgccataga atatccgcgc acctcaacgt
     1261 gacgagaaca ggaaacgacg agaatcagga ccaacagact tttacgcata aaaaagccga
     1321 gccgccgatg ccgcacgcgg cgaagaaaca cacaaatgga aaacgaagag cggcaaacaa
     1381 actccaaccg gaaagcatgc agaatggcat cgcaaaatca accagcagag ccaacagaaa
     1441 aagaagacca aaccatccgg tgctttttgc cggatgcaaa gaacgcagca gatggaacaa
     1501 aataaagaag agaatggcaa atcctatgga atgaggggtc tgggttttca gtatgcccgc
     1561 agtggttttc gctgccagaa aacgatccac acggtccggt tgctgtcggc gtgcggcttc
     1621 ggagcccata taatactcat aattctcgtc ggtgctgatc cccgcttttc ctgcgtagag
     1681 gatgacaccc gtaacaaata caagtcccgc gaagatgctg aacgccgcaa gagggatcct
     1741 gaaatcagcg tacatagagc tttccgacta gaacaactgc agccacagcg aaaagaagcg
     1801 aagccatgcc cgagaaaata cccactgcat tgcatatcat gggaaaccac acgcaaccga
     1861 taacaaggcc tgcatgcaca acggggatga ggaacaaggc ggcggagaat acattcctcc
     1921 atgatttgtc aattagtgtc actgcgatcg aggaaagaaa cagagcttcg attccccgta
     1981 agtagaactg tatgtgaatc tcctccaagg cgagggtcaa ggtttccgga gaggagtgat
     2041 tccagcccaa tatgaaagtc atggcctcga aaatcaaaaa aacaaagcct gccagagaaa
     2101 tcaacaaccg gagacctcct gggcgttgga tctcacccgt gagcaggaat ttcattggag
     2161 gctccgagca tgcaaacggt gaagttccag ggctcttcgc accgcatttt tcgccgcttt
     2221 tactgtcaaa gtggctccgg tgatgggtgg ttgatcgaag gatgatcccg cagttttgcc
     2281 cttgaacaaa gcaaaccacc ggtccggcgc caggtagtca cgtggctcca gaaatgcggc
     2341 gacttggaca tgctttacaa tccccgctgc gtcgaccaca atgaaaagcg tctcttcctt
     2401 ggtccggacc acgtgtgtgt caaaaattcc gaaacctctc acaccgccct gatcactggc
     2461 caggtaaaac gtgtggaccc gcgacgattc gccgcagcac ctagaaagga tttcgccttc
     2521 ttcgcgcgtc aggtaaaggg ttttccgctc aatcgccgtc cccgggaatt cctttttcaa
     2581 aatcatctcc gctgtttcga tcgtctgaga gaaaagcagc acagaccctg ctgcgaaaac
     2641 ggccaatgcg gcgggcaaga cgaagcgaat cggtagtgct cgccgcatca agatacgtat
     2701 cctgtcagaa aataaatcca aggccgaagt tcacctgatc aatcttgttg ttggaagggt
     2761 tcaattgttc gatatccttg tgatagtcgg acttcgaaaa ctgcatagca tagtcgagtt
     2821 tcagcgtaat attctcgtga ggatagaagg ccatacccat agtgtaaata cgtctgtcct
     2881 gcgttctgtc aggaaccccg cgcacaccat agagctcctt gccggagtca gacgcggcca
     2941 cgattccgag agaacgattc ccgttggtca actgagaagt tgtaagacag cccagattcg
     3001 cgcagattaa gtcatcaagg tcttcctttc ctccgggata acgctcgatt gtccgccgct
     3061 gggtgttgac atactccccg cgcacaaaca gcaccagctt tttgggacta ttgaagaagt
     3121 ggagaacatt tacgccgcct tccaaatagc caccttcgac ctccttggca atattcttgt
     3181 tcgcctgctt gttcaaggct cgtgtacctg cgtcactcag ccacccgcga gcaatcactc
     3241 ctcgcgtagt gaaagcaccc ttgtcgacga ggtagtgcgc ttcagcgatt tgaaccggga
     3301 ctctagcgct tgtg
//
LOCUS       DAOQJL010000447         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18750_length_3314_cov_3.782449, whole genome shotgun sequence.
ACCESSION   DAOQJL010000447 DAOQJL010000000
VERSION     DAOQJL010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18750_length_3314_cov_3.782449"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..808
                     /locus_tag="PLG78_15970"
     CDS             113..808
                     /locus_tag="PLG78_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNE24670.1"
                     /translation="MKHLGHRVFGKSVANRDIEVFRYGTGQLSILLMGGVHGDETEGT
                     FFVNKFRTELEKGKIDLPEGITLHICPQANPDGCADNRRTNHRNVDLNRNLPTKDWTG
                     AFTNVRFYPGPHAGSEPENQAILGMIAECMPSVIFSLHSYENPMVNFNGDSEDLGQAM
                     ARANGLPAKGDIGYPTPGSLGTYAGWERNIPTITLEILRGQAHEQVWSAHLEGMNVGL
                     SYYLTHKLPPRKG"
     gene            811..1629
                     /locus_tag="PLG78_15975"
     CDS             811..1629
                     /locus_tag="PLG78_15975"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNE24671.1"
                     /translation="MQNEEIKKLIEEAYDDRTRLPAAREAVLEALSLLNQGKIRVAVC
                     KAPGDWEVQAWVKKAILLYFGITEMKEVHSGDLSYFDKIPTRTDHKEAGIRAVPPAVI
                     RYGAFAEKGVILMPSYVNIGAYVATGTMVDTWATVGSCAQIGKSVHLSGGVGIGGVLE
                     PPQGRPVIIEDGAFLGSRAIVVEGVMIEERAVIGANVTLTASTPIIDVTGPSEVVYKG
                     RVPRDSVVIPGMREKKFAAGTFGVPCALIIGKRKATTDEKTSLNDALRDFEVSV"
     gene            complement(1647..2720)
                     /locus_tag="PLG78_15980"
     CDS             complement(1647..2720)
                     /locus_tag="PLG78_15980"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNE24672.1"
                     /translation="MKKVSELWGKFLRIPLFKVKFGVVRMDNMPFILFHLSPIFIFFV
                     PFKWELVGLAVGLYFVRMFFITGIYHRYFSHRGYEVRNRFIQFVMGLLGTTCVQQGPL
                     WWAEHHRHHHRYSETDNDIHSPVQYGFWHSHMWWFVTMYDNPKYHRYELKDFAKYPEL
                     KWLDNYHVIGPVLLGVALFGIGGAPYLVWGLSVSTVLLWHGTWTINSLSHVFGKKRYE
                     TGDESRNNPFLAILTLGEGWHNNHHAYQGGAKAGFYWYEYDITYYLLWCFHKLGIVTK
                     MGQPPERVLALGRANDAARRAARQLVGRRVLGKLTVEEVQLLVHAAEKGASAFRKKKV
                     GEIKAILASLREKAPMSTMPAMA"
     gene            complement(2717..3112)
                     /locus_tag="PLG78_15985"
     CDS             complement(2717..3112)
                     /locus_tag="PLG78_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24673.1"
                     /translation="MKDNESAWASLIETAIDSKPEPEVPAFLYPRMAKQLFPKPLSRA
                     MQWFVAILLIVSPPLFGYLSDSVYPGGTPDPGIVYTLYGILSLLWVLPVAGGIFHRSG
                     TSLVALSIQIDEIIKHPIRGIRTWSGVSK"
     gene            complement(3109..>3314)
                     /locus_tag="PLG78_15990"
     CDS             complement(3109..>3314)
                     /locus_tag="PLG78_15990"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNE24674.1"
                     /translation="EGDPSVVREELGLLPEVYRLPIVLHYYEKMSIARIAESLDQNEN
                     TVKSYLHRGRKILKQKIEQRKQS"
BASE COUNT          897 a          831 c          827 g          759 t
ORIGIN      
        1 tcaaacgcat gaagagtcta ttgaaagagc ccgtgatctt tgacggaaga aacgtgtatc
       61 cacctcaatc cctcaagaag atgggattcc agtaccaggg tatgggagta gcatgaagca
      121 cctgggacac agggtgtttg gaaagtcagt cgccaatcga gacattgaag tgtttcgcta
      181 tggaaccgga cagttgtcta ttttactgat gggtggcgtt cacggagatg aaacagaagg
      241 cacgttcttt gtgaacaagt ttcgcactga actggagaaa ggaaagatcg acctgcccga
      301 aggaatcact ctgcacattt gcccgcaggc aaatccggac gggtgcgcgg acaacagaag
      361 aaccaatcac agaaatgtgg atctcaatcg caacttacca acaaaagatt ggacgggcgc
      421 atttacgaac gtgcgattct atcctggacc tcatgctgga agcgaacctg aaaaccaggc
      481 aatccttggt atgatcgcag aatgcatgcc ttctgtcatt ttctctcttc actcatacga
      541 aaacccgatg gtgaatttca acggagactc ggaagacctg ggccaggcca tggcaagggc
      601 caatggactc cctgccaaag gagacattgg atacccgact ccaggaagcc ttggaacgta
      661 cgcaggctgg gagagaaaca ttcctacgat cacactggaa attctaagag gccaggcgca
      721 cgagcaagtg tggtctgctc atctggaagg aatgaatgtg ggtctgtctt actacctcac
      781 tcacaaattg cctccgcgca aaggttgatc atgcaaaacg aagaaatcaa gaaactcata
      841 gaagaagcat acgatgacag aaccaggctg cccgcagcac gcgaagcagt actcgaagct
      901 ctttcccttc tcaatcaagg gaagattcgc gtagcggtct gtaaggcgcc cggggactgg
      961 gaagtccagg cctgggtgaa aaaagcaatc cttctctatt ttggaatcac ggaaatgaag
     1021 gaagtgcatt ccggcgatct ttcctacttt gacaagatcc caacacgcac ggatcacaaa
     1081 gaggcgggca ttcgcgcagt tccacccgct gtgattcgct acggcgcatt tgcagaaaaa
     1141 ggcgtcatac tcatgccatc ttacgtgaac attggagctt atgtggcaac aggaaccatg
     1201 gtagacacct gggcgaccgt tgggtcctgc gcgcaaattg gaaaatctgt gcacctgagc
     1261 ggcggagtcg gaatcggtgg agtgcttgaa ccaccccagg gaagaccggt gatcatcgaa
     1321 gacggagctt tccttggatc gcgcgcgatt gtagtggaag gtgtgatgat cgaagaacgc
     1381 gccgtgatcg gggcgaatgt gactctcact gcatctactc ccatcattga tgtaacagga
     1441 ccttcggaag tagtgtacaa aggacgcgtc cccagggatt cagtggtcat tcccggaatg
     1501 cgcgaaaaga aatttgcggc tggaacattt ggagtgccct gcgcactcat cattggaaag
     1561 cgcaaggcaa ctacagatga gaagacatcc ctgaacgatg cgctcaggga ttttgaagtg
     1621 agcgtctaac gtctgcggcc aggccctcag gccatggccg gcattgtgga catcggagct
     1681 ttttcccgga gagaggcaag gatcgctttg atttctccta ctttcttctt cctgaaagca
     1741 gatgctccct tctctgctgc gtgcacgagc agctgaactt cttccaccgt caatttcccg
     1801 agcacccgcc tgcccaccag ctgtcgcgca gcgcgacgag ctgcatcatt tgcacggccc
     1861 agtgctagaa ctctttcagg tggttgtccc attttcgtta caatcccaag cttgtgaaaa
     1921 caccagagaa gatagtaggt aatgtcgtac tcgtaccagt aaaaacctgc ttttgcacca
     1981 ccctgatatg catggtgatt gttatgccat ccttctccca gagtcaaaat ggccagaaac
     2041 ggattgttgc gcgattcgtc tcctgtctcg tatctctttt ttccaaacac atgggagaga
     2101 gagttgatgg tccaggttcc atgccatagc agcacggtag aaacagaaag gccccagacg
     2161 agataaggcg cgccgccaat gccaaagagg gccacaccca gtagaaccgg gccgatcaca
     2221 tgatagttat ccagccattt cagttcagga tatttggcga agtctttcaa ttcgtagcga
     2281 tgatactttg gattgtcata catggtaacg aaccaccaca tatgcgaatg ccagaaacca
     2341 tactgtacgg gggaatgaat gtcattgtcc gtctccgagt agcgatggtg gtgtctgtgg
     2401 tgttccgccc accacagagg cccttgctgc acgcaagttg tgcccagtag tcccatcacg
     2461 aattgaatga aacgatttcg cacctcatac ccgcgatgac tgaaatacct gtgatagatg
     2521 cctgtaataa agaacatccg cacgaaatac aggccgactg ccagaccaac cagttcccat
     2581 ttaaacggaa caaagaagat aaaaatagga gaaagatgga acaggatgaa tggcatattg
     2641 tccattctca ctactccaaa cttcaccttg aataagggaa tgcgaagaaa ctttccccag
     2701 agttcactga ccttcttcat tttgatactc ccgaccatgt tctgattcct cgaattggat
     2761 gctttatgat ttcatcgatc tgaatggaca aagcgacaag gctcgtgcca cttctgtgaa
     2821 agattcctcc cgcgacggga aggacccata gcagagacag aatgccgtat aacgtgtaga
     2881 caattccagg atccggcgtg ccgccaggat aaacagaatc agacaggtat ccgaatagag
     2941 gcggactgac aatcagcaga atcgctacaa accattgcat cgcacgcgaa agtggttttg
     3001 gaaacaattg ttttgccatt cgcggataca ggaatgccgg cacctcaggt tcaggtttcg
     3061 aatcaatggc tgtctctatg aggctggccc acgcggattc attatctttc atgattgttt
     3121 cctctgctct atcttctgct tgagaatctt tcttccgcga tgtaaatacg acttcacagt
     3181 attctcattc tgatccaggc tttctgcaat cctggcaatg gacatctttt catagtaatg
     3241 caaaacaatc ggaagtctgt agacctctgg caacagtccc agctcttcgc gcacaacgga
     3301 tggatctcct tcag
//
LOCUS       DAOQJL010000448         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18784_length_3310_cov_4.557911, whole genome shotgun sequence.
ACCESSION   DAOQJL010000448 DAOQJL010000000
VERSION     DAOQJL010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18784_length_3310_cov_4.557911"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLG78_15995"
     CDS             complement(<1..696)
                     /locus_tag="PLG78_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24675.1"
                     /translation="MNYSAAGKKVLKAIATDILIMRPRGYVLDIISPIPSDLAEQELA
                     NRIKTDLKDEAGIALFNLDTPEIREVDGIVWSPTLGFFLLETKGFQIEDFKAISLVAI
                     NYARENHRRKYGHKPPPWKGAKATARKLASYFDSLYFGRRIFPEAPEPRAQFKDLHIP
                     YISFAVFFPFIREEEFRDRFPQTYPVLEKILIFKDSPLRSKLEAIAHALAPGDKKKGG
                     GDHAEALRVINEYK"
     gene            complement(832..969)
                     /locus_tag="PLG78_16000"
     CDS             complement(832..969)
                     /locus_tag="PLG78_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24676.1"
                     /translation="MAKSMIQELRDGKKKNPGIEVFTRLLKPLGARLVIGDGKREVTV
                     G"
     gene            complement(1126..1836)
                     /locus_tag="PLG78_16005"
     CDS             complement(1126..1836)
                     /locus_tag="PLG78_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE24677.1"
                     /translation="MFEQEKESFHKIKAIAKPFLDWMVDIQHEGLEYVPREGGCILVG
                     NHRSDMDPFIVASVVPRFISWIAAEYTQRIPVFKNLVRETGVIPMQIDGNVSVASIKK
                     TMSVLKSGEILGIFPEGHDYMVKNQFSSPMVDFHQGFATFAARGKAPIIPFVIIPIEE
                     DLETIPVPGYVRSLFGLPEEVCKIPHRVVYKKVKIIFCEPVMYNAFQDVPGQDSILVH
                     HVRSAMEERLTAHGLTQR"
     gene            1873..2667
                     /locus_tag="PLG78_16010"
     CDS             1873..2667
                     /locus_tag="PLG78_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24678.1"
                     /translation="MYPGLLLAFVSTFARVKDHYGRVFVLGRNAEVTMDVKHGGWKPV
                     LPVVEDAILHLAEADALRREGNLEEAVCLLRAIEVMGTGPSKDATRRLENIDRSRAVK
                     DLLAQCDPIVYFRKQTGRTTVQSDTAGLKVSFLGEFRIMKAKSVDSPTLTQKIIYLGH
                     DAESILIAYDLWPRSVSVEDYRRIWDKRRGITSTSTLSDLNAGICWPGRIEAGGPTTA
                     QCAIYEMVEGKQGIEYYETGRLKGIFLAFSGKDRARDALRELRITK"
     gene            2664..>3310
                     /locus_tag="PLG78_16015"
     CDS             2664..>3310
                     /locus_tag="PLG78_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24679.1"
                     /translation="MRTSLRILRFLRRYSTAFLHPDHPFLRDYQERRRLQIIMVTGGL
                     LGVFMLIRGILVIRDTPEIGGIVLTLAFLIFAGMSLIVALRAKGYRWIALALVTVAGG
                     ANYANSYLSRVFPPAGLPFAAAVVVALHFLFNRWMSLLGLVVQIVAFLPFLYQQDFHY
                     ASEGERQFWHTRFFANSAVSLGIIWILCEAYRSVRDQAEIRLNRLKAELARDITLA"
BASE COUNT          773 a          873 c          830 g          834 t
ORIGIN      
        1 tttgtactcg ttaataacgc ggagtgcctc agcatggtct ccaccgccct tcttcttatc
       61 acccggcgcg agagcatggg cgattgcttc gagcttggat cgtaacggac tgtccttgaa
      121 aatgagtatt ttttccagaa ccggatatgt ttgcgggaag cggtctcgga attcttcctc
      181 ccgaatgaac gggaagaaaa ctgcaaagga gatataagga atgtgcaggt ctttgaattg
      241 cgcccgcggt tcaggggcct ccggaaaaat cctccgtccg aagtacagcg aatcgaaata
      301 cgatgcaagt ttgcgcgccg tcgccttcgc gcccttccac ggtggcggtt tgtgtccata
      361 cttgcgccgg tgattctccc tagcatagtt tattgcgacg agcgaaattg ctttaaagtc
      421 ctcaatttgg aatcccttcg tctccaagag aaagaaaccc agtgttgggc tccacactat
      481 cccgtccact tcgcggattt ctggcgtgtc taagttgaac agggcgatgc ccgcctcgtc
      541 cttgaggtca gttttgatcc ggtttgccag ttcttgttca gctaagtccg acgggatagg
      601 tgatattatg tccaaaacgt aacctcgggg cctcattatc aggatatctg tcgcaatcgc
      661 tttcaagact tttttgcccg cagccgaata gttcacgggt gtgcgatcat tgtgcgcggc
      721 accggaatgg gcgcgtccgt ttgagaatcg gagtgggttc cgtccgcctg tggctacacc
      781 cggactctcc ccaggttcca aaacacggca ttgtggaatg tgcaggaaac ctcagccgac
      841 cgttacttcg cgtttgccgt caccaatgac aagacgagcc cccagcggct tcaagaggcg
      901 agtgaatact tcgatgcccg ggttcttctt tttcccatcg cgcaattcct ggatcatgga
      961 cttggccacg cgggattttg tagccagcga ccgcgtggct ccggttgata tctgcaggcc
     1021 gctgcagtta ccgttacttt gccgaaatta cgacctgaga gaagttcagg aaattattcg
     1081 tcgggccatt tgtacccttc ttgcatggcc tagtctaaac gccgcctatc gttgcgttag
     1141 accgtgagcg gtcagtcttt cttccatggc actacgaaca tgatgcacca gaatgctgtc
     1201 ctgacctgga acatcctgaa atgcgttgta catcacaggt tcgcagaata tgattttgac
     1261 ttttttatac acgacgcgat gaggaatctt acacacctct tccggcaaac cgaacagaga
     1321 acgcacgtag ccaggcactg gaattgtttc caggtcctct tcaataggaa tgatcacaaa
     1381 aggaatgata ggcgcttttc cgcgtgccgc gaacgtagca aagccttgat gaaaatctac
     1441 catgggagat gaaaactgat tcttcaccat gtaatcgtgg ccttccggaa atatacccag
     1501 gatctctccc gattttaaaa cgctcatggt cttcttgatg ctggcaacag atacattgcc
     1561 gtctatctgc atgggaatga ctccggtttc tctcaccaga ttcttgaaca ctggaattct
     1621 ctgagtgtac tcagctgcga tccaggaaat gaatcgtggc acaacagacg cgacgatgaa
     1681 aggatccata tcggaacgat ggttgccgac aagaatgcag ccgccttccc ggggcacata
     1741 ctcgagccct tcatgctgga tgtctaccat ccagtccaga aatggctttg caatcgcctt
     1801 gattttgtga aaggattcct tttcttgctc aaacacacct tgattctgaa acgccggaca
     1861 ttcatgtcaa tcatgtatcc aggacttctc ctggctttcg tatctacctt cgctcgggtc
     1921 aaggatcact acggccgcgt gttcgtcctg ggtcgcaacg cagaggtaac catggatgtg
     1981 aagcacggag gctggaaacc agttctgcct gtggtagaag atgcgatcct tcatctcgca
     2041 gaagcggatg cacttcgccg cgaaggaaat ctagaagagg cagtctgtct tttgcgtgcg
     2101 atcgaagtga tgggcacagg tccttctaaa gacgcaacgc gacggctgga aaacattgac
     2161 cgctcccgcg cagtcaaaga tctactcgcg cagtgcgatc caatcgtata tttccgcaaa
     2221 caaacaggga gaaccacggt tcaaagcgat accgccggac tcaaagtatc tttcctcggc
     2281 gaatttagaa tcatgaaggc aaaatctgtc gattcgccca ctctcactca aaagatcatt
     2341 tacctggggc atgatgcaga atccattctc atcgcatacg atctgtggcc gcggtccgtc
     2401 tctgtagaag actatcgccg aatctgggac aaaagacgag gaatcacttc cacaagcacg
     2461 ctgagcgatc tgaacgcagg aatctgctgg ccgggaagaa tcgaggcagg tggaccaact
     2521 accgctcagt gtgcgattta cgaaatggtt gaaggcaaac agggcattga atactacgaa
     2581 actggcagac tgaaaggcat ctttcttgcg ttttccggca aggatcgcgc acgggatgca
     2641 ctgcgagaac ttagaattac aaaatgagaa cttctcttcg catccttcgc tttctcagac
     2701 gttattcgac cgcgttcctt catccggatc atccttttct gcgtgactat caggaaagaa
     2761 gacgcctgca gatcatcatg gtcaccggag gattgcttgg cgtctttatg ctcatccgcg
     2821 gcatcctcgt catccgggac acccccgaga taggcggcat tgtccttacg cttgcgtttt
     2881 tgattttcgc tggaatgagc ctcatcgttg cattgcgcgc gaagggatac agatggatcg
     2941 cccttgcgct tgtgaccgtt gcaggcggag ccaactacgc gaattcatat ctttctagag
     3001 tatttcctcc ggcggggctt ccattcgcgg ccgcagtggt cgtggctttg cactttctat
     3061 tcaaccgctg gatgagcctt ctgggactgg tcgttcagat tgtagcgttc ttaccatttc
     3121 tctaccagca ggatttccac tacgcaagcg aaggcgaacg ccagttctgg cacacacgat
     3181 tcttcgcaaa ctcggcggtc tctctgggca tcatttggat tttgtgcgaa gcatacagat
     3241 ccgttcgtga tcaggcagaa atcaggctca atcgcctcaa agcggaactt gcaagagaca
     3301 tcactctggc
//
LOCUS       DAOQJL010000449         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18864_length_3302_cov_3.343394, whole genome shotgun sequence.
ACCESSION   DAOQJL010000449 DAOQJL010000000
VERSION     DAOQJL010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18864_length_3302_cov_3.343394"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1249)
                     /gene="tyrS"
                     /locus_tag="PLG78_16020"
     CDS             complement(29..1249)
                     /gene="tyrS"
                     /locus_tag="PLG78_16020"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNE24680.1"
                     /translation="MTEKKIPPAIQAAFEKIRRGADEILPEGALIDKLIKSSKTGKPL
                     RIKAGFDPTAPDLHLGHTVLLRKMKHFQELGHEVLFLIGDFTGMIGDPTGRSATRKQM
                     TRAEIEQNAKTYSDQVFRILDKSKTTIRFNSEWCDKMTFADVLGLTAQMTVARMIERD
                     DFTKRLKAGDSISLIEFMYPLVQGYDSVALESDVELGGTDQKFNLLVGRDLQERFGKE
                     GQSILTVPLLVGLDGEKKMSKSYGNYVGITEAPYSMFAKIMSIPDSILRNYFVLLTDY
                     TVAQIEGWIADPFEAKKILAATIVDDYHPAGSGKEARLLWEKEKSERKGLVLPPDAKV
                     LDLKGKSEISLVQAMVDGGVEASLTAARKLIEQGAVKMGDDLETITSRDYVLKLPGNY
                     SFKIGKKKYLVVQG"
     gene            1321..2259
                     /locus_tag="PLG78_16025"
     CDS             1321..2259
                     /locus_tag="PLG78_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNE24681.1"
                     /translation="MTAEEALQLFKDYLIVERDRSQHTVRAYTSDVREFMEFVSRSGI
                     DLFEVEHTDLRAFFAEITGAGMHNGHARKRKLDARSQRRKLASVRTFYSFLVLRDIIS
                     NNPAKEIRSPRMKKNLPGFIPYEEMRTLLEDGPDRQGNLAAERKIRDTALMEILYSSG
                     MRISEALSLNVMNESEDLTQLKILGKGRRERIIFLGDSARAALVHYLKVRDQFRPATQ
                     KLFVNRDGGPLSDRGVRKIMVELQRASGLQRRVHPHRFRHSMATDLLNEGADIRAVQE
                     LLGHRSLASTQVYTGVSKDRLKEVYRRSHPHGRSLD"
     gene            2305..2838
                     /gene="hslV"
                     /locus_tag="PLG78_16030"
     CDS             2305..2838
                     /gene="hslV"
                     /locus_tag="PLG78_16030"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNE24682.1"
                     /translation="MSDFHSTTILCVRRDNRTVIGGDGQVSLGPTIMKSGARKIRKLA
                     GREILSGFAGASADAFTLFELFEKKVEQYQNNVGRAAVELAREWRTDRMLRRLEAMLV
                     VADNKEMYLISGTGDVIAPDDGVLAVGSGGNYALASARALVANTQMSAREIVEKSLQI
                     AADICIYTNHNIAIEEL"
     gene            2835..>3302
                     /locus_tag="PLG78_16035"
     CDS             2835..>3302
                     /locus_tag="PLG78_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE24683.1"
                     /translation="MNDLDHLTPAQIVSELDKYIVGQNAAKKAVAIAIRNRIRRRKLP
                     PEIQEEIYPKNILMIGPTGCGKTEIARRMSRLLNAPFLKVEATKYTEVGYVGRDVESM
                     VRDLLNTAISLVKKEYREKVEVDEKNAEEERLLDLLLPSSSPHKEPAAGSNFSD"
BASE COUNT          820 a          805 c          851 g          826 t
ORIGIN      
        1 ggaacgaccg gggcgctgaa gagcggtttc acccctgaac aaccaggtat tttttctttc
       61 cgattttgaa agagtaatta cctggcagtt tcagcacata gtctctggac gtaatggttt
      121 cgagatcgtc tcccattttt acggcgccct gctcaatgag ttttcgcgct gccgtaagac
      181 tggcctccac tccgccgtcc accattgcct gaaccaggct gatttctgat ttccctttca
      241 ggtcgagcac ttttgcgtcc ggcggcaaaa ccagtccctt tcgttcgctt ttttcttttt
      301 cccagagcaa tcgcgcttct ttgccgctgc cggcaggatg gtagtcatct acgatggtag
      361 ccgccaggat tttttttgcc tcgaacggat ctgcaatcca gccttcaatt tgtgcaaccg
      421 tataatccgt aagtagtacg aaatagtttc tcaaaataga atctggaatc gacatgattt
      481 tcgcaaacat gctgtaaggt gcttccgtaa tcccaacgta gttcccgtag gacttggaca
      541 ttttcttttc gccgtcgagg cctacgagta gagggacagt gaggatggac tgaccctctt
      601 ttccaaatcg ttcctgcaag tcgcgtccaa cgagtagatt gaacttttga tccgttcctc
      661 cgagctccac atctgattcg agcgcaaccg agtcatagcc ctgaacaagt ggatacatga
      721 actcgatgag gctgatggaa tcgcctgcct tgagcctttt tgtgaagtcg tcgcgctcaa
      781 tcattcgagc cactgtcatt tgtgcggtca gacccagaac atctgcgaaa gtcattttat
      841 cgcaccattc tgaattaaag cgaatggttg ttttggattt gtccagaatg cgaaatacct
      901 gatctgagta agtctttgcg ttttgttcaa tttccgcgcg agtcatctgc tttcgcgtcg
      961 cagatcttcc cgttggatca ccgatcattc ctgtgaagtc gcctatgaga aacagtacct
     1021 catgacccag ttcctggaaa tgtttcatct ttcgcagaag cacagtgtga cccaggtgca
     1081 gatccggtgc ggtaggatcg aagcccgcct tgatccgaag cggtttgcca gtcttgctgc
     1141 tcttgatcag cttgtctatg agagcccctt caggcaggat ttcgtctgcg ccccggcgga
     1201 ttttttcgaa ggcagcctga atggcgggag gaatcttttt ctctgtcatg ggtcctttca
     1261 ccgggccgat ctgagggcag ttgtcaaacc aaatccgctg tgacctgccg ataatcgaat
     1321 atgaccgccg aggaagcatt gcaactgttc aaagactacc tcatcgtgga gcgcgatcgg
     1381 tcacagcata cagttcgagc gtatacttcg gatgtgcgag aattcatgga gtttgtttcc
     1441 cggtcaggaa tcgatctgtt tgaggtagag cacacggacc tccgcgcctt ttttgctgaa
     1501 atcacgggtg ccggaatgca caacggccat gccagaaaac ggaaactgga cgcaagatcg
     1561 caaagacgca aacttgcaag cgtgcgtaca ttttattcgt ttctggttct gcgcgacatc
     1621 ataagcaaca atccggcaaa agaaatacga tctcctcgca tgaaaaagaa tctaccaggc
     1681 ttcattcctt acgaagaaat gcgcactcta ttggaagatg gtccggatcg tcagggcaat
     1741 ctggcggcgg aaagaaaaat tcgagacaca gcacttatgg agattcttta ttcgagtgga
     1801 atgcgaattt ccgaagcact ctctctgaac gtgatgaatg aatcggaaga tctcactcag
     1861 cttaagattc tgggaaaggg tagaagagag agaattattt ttctgggcga ctctgccagg
     1921 gctgctctcg tgcattacct caaagtgcgc gatcagtttc gtccggcaac gcagaagttg
     1981 tttgtgaatc gcgacggcgg cccactgagc gatcgcggcg tgcgaaagat catggtagaa
     2041 ttgcagagag cttcaggact gcagaggcga gttcatcctc atcggtttcg tcatagcatg
     2101 gctactgatt tgctcaacga aggcgcggac attcgcgcag tgcaggagtt gcttggacac
     2161 cgcagcctgg caagcactca ggtatataca ggagtttcaa aggatcgtct caaagaagtg
     2221 tatcgacggt ctcatccaca cggccggtct ctggactgaa cctaaaacgg tttgcccgcc
     2281 ggagagactc tgtgaaaaag gactatgtcc gattttcatt cgactacaat tctatgtgtg
     2341 cgccgcgaca atcgcactgt gattggcgga gatggtcagg tcagtcttgg ccccacgatc
     2401 atgaaaagtg gagcgcgcaa aattagaaaa ctcgccggca gagagatact ttctggattc
     2461 gccggtgcgt ctgcggatgc ttttactctc tttgaattgt ttgagaaaaa agtagaacag
     2521 tatcagaaca atgtgggtcg cgctgctgtg gaactggcgc gggaatggcg cacggacaga
     2581 atgctgcgaa gactcgaggc aatgctcgtc gtcgcggaca acaaagaaat gtatctgatc
     2641 agcggaacag gggacgtcat tgctccagat gacggagtgc tcgcagtggg ctccggtggg
     2701 aactatgctc ttgcttccgc gcgtgctcta gttgcgaata ctcaaatgtc tgccagagaa
     2761 atcgtggaga aatcgttaca gattgcggcg gatatctgca tttatacaaa tcacaacatt
     2821 gcaattgagg aactatgaac gatctcgatc atttgactcc cgcgcagatc gtaagcgaac
     2881 tcgataagta catcgtcggg caaaacgcag cgaagaaggc cgtggccatt gcgattcgca
     2941 atagaatcag aagacgcaaa ctgcctccgg agattcagga agagatctat cccaaaaaca
     3001 ttctgatgat cggtcctacc ggatgcggaa aaactgaaat tgcgcgaaga atgtcacgcc
     3061 tgctcaatgc tcccttttta aaagtggaag caaccaagta cacggaagtg ggctacgtgg
     3121 gtcgggacgt tgagtccatg gtgcgcgatt tgctcaacac tgctatctct ctggtaaaga
     3181 aggaataccg cgagaaggtg gaggtggacg aaaagaacgc agaagaggaa agactgctcg
     3241 atcttctact tccatcttcc tctccccata aggaacccgc cgcaggatct aatttttcgg
     3301 ac
//
LOCUS       DAOQJL010000450         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18871_length_3301_cov_3.902033, whole genome shotgun sequence.
ACCESSION   DAOQJL010000450 DAOQJL010000000
VERSION     DAOQJL010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18871_length_3301_cov_3.902033"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PLG78_16040"
     CDS             <1..1309
                     /locus_tag="PLG78_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24684.1"
                     /translation="SKVIPKYKGRGSLKSELEQVQLEEAAYYLNENVPRSAEFLSRRG
                     ALIKKTILSDPNRKGGVRYPGSLAVETLLLVEPFSRQKYADQWKAEVASQHHSFKERL
                     KKESNHWSDLILFLEEKDALSFPKEVWKRITEDKEILHHTWEKAGSTLHVFVRKDPEV
                     FKVFVEGMTDLPVGEHWKILAMKSLLDRYETNFSQLFHDELFVRSYGKLLRIAYIDYI
                     PWYYRIFIWLGLNWFQDKSFQIAKGAISIEQKYLAGSNQQKSAELSQARETEKRQMMT
                     RIQEMSAANQIVDALDRAYHTDLWIPSVAEVQASIKDLDPSSFREALTRDRFQIVRDG
                     KDSPDDILVYPMNHEWRVRAARLRRALDRIMAKYPEGMQSDESRTMHERIRKLTRFLS
                     RKEKEIPALDREDPYRKFEKALKTHEQKAPKTASSPVAEDLEV"
     gene            1377..2282
                     /locus_tag="PLG78_16045"
     CDS             1377..2282
                     /locus_tag="PLG78_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24685.1"
                     /translation="MTGDTKDLEKEVKALRAEIAAFGESPYLKSSIKNLVYEAVIDRT
                     EVERNELGKRINEKHGTMYEIKEQARRMAILLGLDADNIRLLVTEAVQNILEHGSGRW
                     VQVRLDARNEGENPALVCAFKHEIPDSEKYTLQDAEANALKGDVTSEFFDFESPRGRG
                     EFIMKQLTDERRIINGVEINRDGKKVHYFKRILINYRNPEGVRDQLSFQEIKDQIDRL
                     GYQDVVCCFLIQHSFDKPDLFTVACSRDREKQVVQVMTHHSCSLVEQEAYFSNVFATF
                     APERPLTSAELQIVFDEVKSIVYGD"
     gene            2282..3028
                     /locus_tag="PLG78_16050"
     CDS             2282..3028
                     /locus_tag="PLG78_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE24686.1"
                     /translation="MDTGLKDSVVLVTGGTKGIGLAIAQAFQAEGAKVCICARSQEGL
                     DQCRERYALSGYQCDVTSAPSRKNLVESVLGDFGRIDVLVNNAGGSNGSTAMNTDLSL
                     YEEALQLNFLSLVDLSKQVAARMEKGVILNITSISGRESGGRATYNAAKAAAISFTKS
                     FADEMIRKGIRVVGIAPGSILHPTGNWQKRINENPEKMREFVEREIPAGRFGTVEEVA
                     SVAVFLASSKASWVVGATLNVDGGQSRMNF"
     gene            complement(3030..>3301)
                     /locus_tag="PLG78_16055"
     CDS             complement(3030..>3301)
                     /locus_tag="PLG78_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24687.1"
                     /translation="KGKLEPVSLYEVVADKPRNSVRISQIPAFENAVSLYQKGDLSAA
                     RKQFIELYKENTEDRAVRLYVRRIDEFLKNGLPENWDGVETLTSK"
BASE COUNT          884 a          742 c          877 g          798 t
ORIGIN      
        1 atctaaagtg atcccaaagt ataagggtcg cggatcgctc aaatctgagc tggagcaggt
       61 tcagcttgag gaagcggcct actatctaaa tgaaaacgtc ccgcgctccg ccgagtttct
      121 ctccagacgt ggcgccttga tcaagaagac aattctgagt gatccaaatc gaaagggtgg
      181 tgtgcgatac cccggatctc tcgcagtcga aacgcttctt ctagtagagc cattctctcg
      241 ccagaagtat gcggaccagt ggaaagcgga agtggcttcg cagcaccatt cattcaaaga
      301 gcgcctcaag aaagaatcca atcactggag cgatttgatt ttgtttttgg aagaaaagga
      361 tgctctgtct tttccaaagg aagtgtggaa gagaatcacg gaagataagg aaatattgca
      421 tcatacctgg gaaaaggcag gcagtactct gcacgttttt gtgcgcaaag atccagaagt
      481 tttcaaagtg tttgtagaag gaatgacaga cctgcctgtg ggcgaacact ggaagattct
      541 cgcaatgaag tctctgctgg accggtacga gacaaatttc agccagctat ttcacgatga
      601 gttgtttgtg cgaagctatg gaaagcttct gcgcattgcg tacattgatt atataccctg
      661 gtattatcgc atttttatct ggttgggctt gaactggttt caggacaaat cctttcaaat
      721 tgccaaaggc gcaatatcga ttgagcagaa atatctagct ggttcgaacc agcagaaatc
      781 cgcagagcta tcgcaggcca gggaaacaga aaagcgacag atgatgacgc ggattcaaga
      841 aatgtctgct gcaaatcaaa tcgtggatgc gctcgaccga gcgtatcata ctgatttgtg
      901 gataccttcg gtagcggaag tgcaagcttc gatcaaagat ctcgatccat cctcttttcg
      961 tgaggcactc acgcgtgatc gtttccagat tgtgcgcgat ggcaaagact caccggacga
     1021 tattcttgtt tatccaatga atcatgaatg gcgcgttcgc gctgcccgcc tgcggcgagc
     1081 gttagatcgc atcatggcca aataccccga aggcatgcag tcggatgagt ctcgcaccat
     1141 gcacgagcgc atccgtaaac tcacccgatt tctgagtcgc aaagaaaagg aaatccccgc
     1201 tctcgaccga gaagacccgt atagaaaatt tgagaaagct cttaaaacgc atgaacaaaa
     1261 ggctccgaag acagcatctt cgcctgtagc tgaggacctg gaggtctagg gctcgcgctg
     1321 gaaaaatact ttgacaaatg gcgggcttcg ccggatgttc tggaaggggg aacgctttga
     1381 ccggagacac aaaagatctc gagaaagaag tcaaagcgct tcgcgcagag attgctgcgt
     1441 tcggggaatc gccctatctg aagagttcca tcaagaatct agtctacgaa gctgtgattg
     1501 atcgcacaga agtagagcgc aatgaacttg ggaagcgcat caatgaaaag cacggcacaa
     1561 tgtatgagat caaagagcag gccaggagaa tggctatcct gcttggtctc gatgcggaca
     1621 atattcgtct cctcgtaacg gaagcagtgc aaaatattct cgaacacgga tccggtcgat
     1681 gggttcaggt ccgcctggac gcaagaaatg aaggtgaaaa cccggccctc gtttgtgctt
     1741 ttaagcatga gattcctgat tctgaaaaat acacactcca ggatgcggaa gcgaatgctc
     1801 tcaaaggaga cgtgacttcc gagttttttg acttcgaatc cccgcgtggt cggggcgaat
     1861 tcatcatgaa gcaactaaca gatgaaaggc ggatcatcaa cggcgttgag atcaatcgtg
     1921 atggaaaaaa ggtgcactac ttcaaacgca tactgatcaa ctataggaat cctgagggag
     1981 tgcgtgatca gctttctttt caagagatca aagaccagat cgatagactc ggatatcagg
     2041 atgtggtctg ctgctttttg attcaacatt cgtttgataa gcctgatttg tttacagtgg
     2101 cctgctctag agatcgtgag aagcaagtcg tccaggtaat gacccaccac agttgttcgc
     2161 ttgtggagca ggaagcgtat tttagcaatg tattcgcaac atttgcgccc gaaagaccac
     2221 ttacaagtgc ggagctgcag atagtctttg atgaagtaaa atcgatcgtc tacggtgact
     2281 aatggatacc gggcttaagg attccgttgt ccttgtgacc gggggcacaa aggggattgg
     2341 actggccatc gctcaggcgt ttcaggcgga aggcgcaaaa gtttgtatct gcgccaggtc
     2401 gcaggaaggc ctggatcagt gccgggaacg ttatgcgctt tccggttacc agtgcgatgt
     2461 gaccagtgct ccttcgcgca agaatcttgt ggaatccgtt ctgggcgatt tcggtcgcat
     2521 agacgtactg gtaaacaatg caggcggatc gaatggatcg actgctatga atacggatct
     2581 ttccctttat gaggaagcgc tgcagctgaa ctttctttcg ctcgtggatt tatccaaaca
     2641 ggtagctgcc cgcatggaga aaggagtgat cttgaatatc acatccattt caggtagaga
     2701 atcgggcggt cgcgcgacat acaatgccgc gaaagcggct gccattagtt ttacaaaatc
     2761 gtttgcagat gaaatgatcc gaaagggaat ccgggtggtt gggattgcgc ctggctccat
     2821 ccttcatcct accgggaact ggcaaaaaag gatcaacgaa aacccggaga aaatgcgaga
     2881 gtttgtagaa cgcgaaattc ctgcgggaag atttggaaca gtcgaagagg tggccagtgt
     2941 tgccgtattc cttgcgtcga gcaaggcttc ctgggttgtg ggcgcgacgc tcaatgtgga
     3001 cggcgggcag tccagaatga acttctaaat tacttgctgg tcagtgtttc tacgccatcc
     3061 cagttttcgg gcagtccatt cttcaagaat tcgtcgattc tcctgacgta gagtcgcact
     3121 gcgcgatctt ccgtattttc tttgtagagt tcgatgaatt gctttcgcgc cgcagagaga
     3181 tcccccttct gatacaggga aacagcattt tcaaaggctg gaatctgcga aattctgacg
     3241 gaattgcgcg gtttgtccgc cacaacttca tagagagaaa caggttctaa ttttcctttc
     3301 a
//
LOCUS       DAOQJL010000451         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_18939_length_3294_cov_3.783575, whole genome shotgun sequence.
ACCESSION   DAOQJL010000451 DAOQJL010000000
VERSION     DAOQJL010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18939_length_3294_cov_3.783575"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PLG78_16060"
     CDS             complement(<1..909)
                     /locus_tag="PLG78_16060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE24688.1"
                     /translation="MNTRFFPGLLIVLSLAFLVPGTGGKDFPGQGDEVMHLVTVQQSL
                     ASKSYIVPVVDGVPNYYKPPLLFWAGMVTEAVLGPGLFGPRFSSILFTTGIVLLMYYL
                     MVVWGVRPWVAVVASIAYLCSIGTMKFGRLLMMEQGMAFFYLLFVFLLLEHYRTGRIR
                     PLIVSGLISGIGFLFKGPVFLIYCAFTLIIWASMRIVRFKTDPVRITGLRKFWPTVRT
                     GLIFSAFALVVPVIWAGSVLLFAGVAGKAMLAYFFIVENAGKFAQQDQSEIRILVGWI
                     IYTFPWTVVLFAGLYFALRSRYAYGKV"
     gene            1040..2746
                     /locus_tag="PLG78_16065"
     CDS             1040..2746
                     /locus_tag="PLG78_16065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE24689.1"
                     /translation="MNNPLIVQSDRSMLLEVDHELFDACRAAISRFAELEKSPEYLHT
                     YRVTPLSLWNAASSGMNAEEIVAALEQYSRYPVPKNVIAEVREQISRYGKVKLIKEDD
                     GTLLIISNERAFLNEIAMHRNVQPFIEGRRGDSIIVKKQYRGHIKQALIKIGYPVEDL
                     AGYDEGAKYGFNFRGTTLSGKTFGMRDYQRKSVEVFHASGSREGGSGVIVLPCGAGKT
                     IVGIGVMQIVGAQTLILVTNTLSIRQWKNEILDKTDIPEEDIGEYSGEKKEIKPITIA
                     TYNIITHRKKKDGDFTHFDIFSANNWGLIVYDEVHLLPAPVFRMTSELQAKRRLGLTA
                     TLVREDGLEEDVFSLIGPKKYDVPWKDLERSAFIATAKCIEIRVDLDEELRTRYGIAD
                     DREKYRLASENPDKIKVIERILEEHGRINVLIIGQYLDQLDKLAAHFNFPLITGKTPL
                     PEREFLYAGFRTGKVPCLIVSRVANFSIDLPDASVAIQVSGTFGSRQEEAQRLGRVLR
                     PKPDDNMAYFYSLVSRDTTEERFGQNRQLFLTEQGYEYQIFTFEQYRELAGTAKATIL
                     SR"
     gene            2757..>3294
                     /locus_tag="PLG78_16070"
     CDS             2757..>3294
                     /locus_tag="PLG78_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24690.1"
                     /translation="MANGKRPFVVFFLVAASACIVALFFYAGITHPPTADSDDILRKV
                     PPGTTHIRVGVPTWGGFAGGQYFNEGFNPSTESRYYKNFRILVSFVVMDDIPASREVW
                     KRGDIDVISATADSLAPEIETFLSEEPRVIFQSDWSRGGDAIIVDRSIHMPEELKGKR
                     VAFLPASPSHTLLLHVLDT"
BASE COUNT          950 a          856 c          809 g          679 t
ORIGIN      
        1 aaccttcccg tacgcatatc tgctgcgaag agcgaaatac aatcccgcaa aaagaacgac
       61 agtccagggg aaagtgtaga taatccaacc aaccagaatt cgaatctcag attgatcctg
      121 ctgggcaaat ttccccgcgt tctcaacgat aaagaagtac gcaagcattg ctttgcccgc
      181 gactcctgca aaaaggagta cggaccccgc ccagatcacc ggaacaacga gcgcgaaggc
      241 cgagaagatc aaaccagtcc gcacggttgg ccagaacttc cgcaaacctg tgattcgcac
      301 aggatcagtt ttgaatctaa cgattcgcat gcttgcccag atgatcagtg taaaagcgca
      361 atagatcaaa aagacagggc ctttgaatag aaagccaatc cctgaaatca agccgctgac
      421 aatcaggggt ctgattctgc ctgtgcgata atgctcaagc aacagaaaca caaacagaag
      481 atagaagaag gccattccct gctccatcat gagaaggcga ccaaacttca tggtgccgat
      541 cgagcagaga taagcaatag atgcgaccac ggctacccag ggacgaacgc cccagacaac
      601 cattaagtaa tacataagaa gaacaattcc ggttgtaaaa agaatggagg agaatcgcgg
      661 accaaaaagc cccgggccca ggacagcttc ggtgaccatt cccgcccaga agagaagcgg
      721 tggcttataa taattgggaa cgccgtccac aacaggcacg atgtaagatt tcgaagcgag
      781 actctgctgg actgtaacca gatgcatcac ctcatcgccc tgaccgggaa aatccttacc
      841 gccggtgccc ggaaccagga aggcaagaga gagcacgatc agtagaccag ggaaaaatcg
      901 agtgttcatg agtgctggat cagaaaaaca acggggcgaa ccctggtcac tttttttctt
      961 taactatgcg tagaaaacag gcggcttaaa aggcttgacc gcacgcattt ttttcggggg
     1021 tatggccacg atgccggata tcaacaatcc tctaatcgtt caatcagatc gcagcatgct
     1081 gctcgaagta gaccacgagc tatttgacgc gtgccgcgca gccatcagca ggttcgcaga
     1141 gcttgaaaaa agccccgaat atctgcatac gtatcgtgtg actcctctct ccctgtggaa
     1201 tgcagcgtcg agcggaatga atgcggaaga aattgtggcc gccctggaac aatactcacg
     1261 ctacccggtt cccaaaaatg tgatcgcaga agtgcgcgaa caaatctcac gttacggcaa
     1321 agtaaaactc atcaaagaag acgatgggac tctactcatt atctccaatg agagagcgtt
     1381 cctcaatgaa attgccatgc atcgcaacgt gcaacccttc atcgaaggaa gacgcggaga
     1441 cagcatcatc gtcaaaaagc aataccgcgg tcacatcaag caagcactca ttaagatcgg
     1501 ctaccctgtg gaagacctgg caggctatga cgaaggagca aagtacggat tcaatttccg
     1561 cggtacaact ctttccggga agacattcgg aatgcgcgac taccagcgca agtcagtaga
     1621 agtatttcat gcatccggtt cacgcgaagg cggatctgga gtgatcgttc ttccttgcgg
     1681 cgcaggcaag acgattgtgg gaatcggcgt gatgcagatt gttggcgcac aaactctgat
     1741 tcttgtaacc aacacattat ccatccgcca gtggaaaaat gaaatcctgg acaaaacgga
     1801 tatccctgaa gaggacatcg gcgaatactc aggggaaaag aaagaaatca agccaatcac
     1861 aattgcgact tacaacatca tcactcatag aaagaagaaa gacggggact tcactcactt
     1921 tgacattttt agcgcgaaca actggggcct catcgtatat gatgaagtgc acctccttcc
     1981 cgcacctgta ttccgcatga cgtctgaact tcaggcaaaa agaaggctcg gcctcaccgc
     2041 caccctggtc agagaagatg gactggaaga agacgtattc tctctgattg gtccaaaaaa
     2101 gtacgatgta ccctggaagg acctcgagcg aagcgctttc atcgccacgg caaaatgcat
     2161 tgaaattcgc gtggatctag acgaagagct tcgcactcgc tacgggattg cggacgaccg
     2221 cgaaaagtat cgtctcgcaa gcgaaaaccc ggacaagatc aaagtcattg aaagaattct
     2281 agaagaacac gggcgcatca acgtgttgat cattggtcag tacctggacc agctcgacaa
     2341 gctcgcggcg cacttcaact tccctctcat tacaggaaaa actccgctgc ctgaaagaga
     2401 atttctctac gcaggattca gaacggggaa agttccttgc ttgattgtat cccgcgttgc
     2461 gaacttttcc attgaccttc cagacgcgtc cgttgccatc caggtcagtg gaacgtttgg
     2521 ttccagacag gaagaagcgc agagactcgg ccgcgttctt cgcccaaagc cagacgacaa
     2581 catggcatac ttctactcac tggtaagccg cgacaccacg gaagaaagat tcggtcagaa
     2641 tagacagctc ttcctgacag agcaaggcta cgaataccag atcttcacct tcgaacaata
     2701 cagggaactc gcgggcaccg cgaaagctac gattctgagt agatagaggc ggaagtatgg
     2761 cgaatggaaa aaggcccttt gtagtctttt tcctggtggc agcaagcgca tgcattgttg
     2821 ctctgttctt ctacgctggc attactcatc caccgactgc agactcggac gatatcctgc
     2881 gaaaggttcc gcccggaacg actcacattc gcgtaggagt tcctacatgg ggtggctttg
     2941 ccggagggca gtactttaat gaaggcttta acccttccac tgaatcgcgt tactataaaa
     3001 actttaggat tcttgtttct ttcgttgtga tggacgacat cccggcttcg cgtgaagtat
     3061 ggaaacgggg agacattgac gtaatctccg caactgcaga ttccctggcc cctgagattg
     3121 aaaccttcct atcagaagaa ccccgggtga tcttccagtc agactggagc cgtggcgggg
     3181 acgcgatcat cgtggaccgc tccattcaca tgcctgagga gttgaaaggc aaacgcgtag
     3241 catttcttcc cgcttcgccc tcgcacactc tgttgcttca tgtgctcgac accg
//
LOCUS       DAOQJL010000452         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19131_length_3271_cov_5.114428, whole genome shotgun sequence.
ACCESSION   DAOQJL010000452 DAOQJL010000000
VERSION     DAOQJL010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19131_length_3271_cov_5.114428"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLG78_16075"
     CDS             <1..555
                     /locus_tag="PLG78_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNE24691.1"
                     /translation="VLPNGDVVLAGENETYVLLGEPHVTMERDKSGHAFVEFQIKQED
                     HASLVVAFLLRATLWMILYGIVFGLLSLGYKYIPTHPISRESAAAGGLFTATTLIGFV
                     IGFQVWVTGFANTGRLYGVWAVIPVGMILLIFCTQILFFGLELACVLDERRTQDAYPE
                     AGGVRLSVPLKVEVPLVRAKKPRK"
     gene            559..1350
                     /locus_tag="PLG78_16080"
     CDS             559..1350
                     /locus_tag="PLG78_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE24692.1"
                     /translation="MDVSHLFIWESRVLFLAKGLRAEAHAHYNASLIISADKPFEIRT
                     PERGYGGLECVLISPNVYHELRAEKSSMIVLQIDPLGAEYRALANALGGEGIIQAQRP
                     DGIEKELSILLDPASSRCESAAAFVRMLLMRTIHYEEMPHTVDPRVARLIALLKEHIS
                     EDMPETVSVQEAAAHVGLSADRLMHLFKEEMGLPIRRYVLWLKLRRAVMLMRTEGIRM
                     TEASHAAGFADSAHLSRTFKDMFGIPPSFFFANDRFVQVNQCEVW"
     gene            1375..1851
                     /locus_tag="PLG78_16085"
     CDS             1375..1851
                     /locus_tag="PLG78_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNE24693.1"
                     /translation="MKNVQQWFDEYAESHRNETNQTIHWICIPLIMFSTLGLFWAASD
                     YVVSATGISLPYYVNLASLLLLVGMTWYLVMSVRLFLAMVPVAALMVYGLWILKINAM
                     PVAVICGAIWISAWIGQFIGHKIEGKKPSFFKDIQFLLVGPFWLMGKLFKKLGIAY"
     gene            complement(1868..3010)
                     /locus_tag="PLG78_16090"
     CDS             complement(1868..3010)
                     /locus_tag="PLG78_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24694.1"
                     /translation="MALLCAYYFYSVSHKPVLHFQENEFHDQILREVPVLTRAYHPTP
                     WCFNNHLMLLLLLFREYRSKDYPFDRIDQLRMKDGGITGLAWSGWNEQNALLKNKKKS
                     IMPIVVIFHTISGDEQDVKGIVEYVRVSLRWTAVVCIRRGHGNLPLGKPKINTMGSTS
                     DLSEQLAFIQKKFPGAPLFGIGISAGSGLLARYLGESGKKSKFMAAAAISPAYDIEKA
                     FHRIHPVYSKIMGQRLISYFLKRHYDSLSRVHGFDHAMQAQTIGEFQDRIHAIAGYKT
                     KEEYYRFSNPIKVAKNIRTPLLILNSQDDPICVNLNVLENLHWLENLPHTMLVHTKRG
                     SHVAYFEGWKAKPWCDRVVGDYFQAVLKTEAPTAKRRPSKKKQVRR"
     gene            3030..>3271
                     /locus_tag="PLG78_16095"
     CDS             3030..>3271
                     /locus_tag="PLG78_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24695.1"
                     /translation="MVSTIGSSVLLLKSEVQNLLQPTSHFLTCGCSVLKYEGHVSLVL
                     LIDADPNQVSKFEHAMQGSEVRLLTASNLARGLELA"
BASE COUNT          752 a          756 c          837 g          926 t
ORIGIN      
        1 gttttaccaa acggcgatgt ggttctggcc ggagaaaacg aaacgtacgt gttactcgga
       61 gagccacacg tgactatgga aagagacaaa agcggtcatg cattcgttga gtttcaaatc
      121 aaacaggaag atcatgcgtc tcttgtggtt gcctttcttc ttcgcgccac tctgtggatg
      181 attctctacg gaatcgtttt tggtctgctt tctcttggtt ataagtacat ccccactcat
      241 ccaatttcca gagagtcggc ggcagcgggt ggactcttca ctgccaccac cttgattgga
      301 tttgtaattg gatttcaggt ctgggtgact ggatttgcaa atacgggtag actctatgga
      361 gtgtgggctg tgatccccgt cgggatgatc cttctgattt tctgcactca gattcttttc
      421 tttgggctgg agcttgcctg tgttctagat gagcgtcgca cgcaggacgc atatcccgag
      481 gcgggcggag tgagactcag cgttcctctg aaagtggaag tcccactcgt gcgtgcgaag
      541 aaaccgcgca agtgagtcat ggacgtttcg catctcttta tctgggaaag cagggtactg
      601 tttctcgcaa aaggccttcg cgcagaagcg cacgctcatt acaatgcctc tttgattatt
      661 tccgcagata agccttttga aattcgcact cccgagcgcg gctacggtgg cctcgaatgc
      721 gttttaatat cgcctaacgt ataccacgaa cttcgcgcag agaaaagctc catgattgtg
      781 ctgcaaattg atccactggg tgcagaatat agagcgcttg caaatgcact gggaggagag
      841 ggcatcattc aagcacagcg acccgatggc attgaaaagg agctttctat tcttctggat
      901 ccggcctctt cccgctgtga gtccgcggcg gcctttgttc gcatgcttct catgcgaaca
      961 attcactacg aagagatgcc tcatactgta gatccacggg tggccaggtt gatcgcctta
     1021 ctcaaggaac acatcagcga agacatgcca gaaactgtgt ccgtgcagga agcggccgct
     1081 catgttggac tgtctgcgga tcgtttgatg catttgttta aggaagaaat gggcctgccc
     1141 attcgcagat atgtgctgtg gctcaagctt cgccgggcag tcatgttaat gcgcacagag
     1201 ggaattcgga tgacggaggc ttcgcatgcg gccggttttg cggattctgc gcacttgagc
     1261 cgaactttca aagatatgtt tggcattcct ccttcatttt ttttcgcaaa tgacagattc
     1321 gttcaagtga atcagtgcga agtgtggtag agttcatcat cgggggttat gatcatgaaa
     1381 aacgttcaac aatggttcga tgaatacgca gaaagccaca gaaatgagac caaccagacc
     1441 atacactgga tctgcattcc tctcattatg ttcagtactc ttggattgtt ctgggctgcc
     1501 tctgactatg tggtttcagc caccggcatt tctttgccat actatgtgaa cctggccagc
     1561 ttgcttttgc tcgtagggat gacctggtat ctggtgatgt ccgtgagatt gtttctggca
     1621 atggttcctg tggcagcgct gatggtgtat gggctctgga ttctgaagat caacgcgatg
     1681 cctgtggcag tgatctgcgg agcgatctgg atttctgcct ggattggcca gttcattggc
     1741 cacaagatag aaggaaagaa gccttctttc ttcaaggaca ttcagttttt gctggtgggc
     1801 cctttctggc tgatgggaaa gctatttaag aaactgggaa ttgcgtactg agcgttctca
     1861 caaacgatta ccttcgcact tgtttctttt tcgatgggcg ccttttcgca gtgggcgcct
     1921 cagtttttag aactgcctgg aagtagtctc ccacaacccg gtcgcaccag ggcttcgcct
     1981 tccatccttc gaaataagcg acatgacttc ctctcttcgt gtgaacgagc atagtgtgtg
     2041 gaagattctc gagccagtgc aagttctcaa gcacgtttag atttacgcaa atgggatcat
     2101 cttgtgagtt cagtatgaga agtggagtgc gaatgttctt tgccactttg atcggattcg
     2161 aaaatcggta gtattcttct tttgttttgt atcctgcgat tgcatggatt ctgtcctgaa
     2221 actcgccaat ggtttgcgcc tgcattgcat gatcgaagcc gtgcactcta gaaaggctgt
     2281 cataatgtct ctttagaaaa tagctgatca atcgttggcc catgattttg ctgtagactg
     2341 gatgaatgcg atgaaatgct ttttcaatgt cgtaagcggg agaaatggct gcggcagcca
     2401 tgaatttact cttcttgccg gactcgccca gatatctggc aagcaaacct gacccggctg
     2461 aaattccaat gccgaataac ggtgctccag ggaatttttt ctgaatgaat gcaagttgct
     2521 ctgaaaggtc agatgtggaa cccattgtgt tgatctttgg ttttccaagc ggaaggtttc
     2581 cgtgtcctcg cctgatgcag accactgcag tccaacgcag ggatacgcgt acatactcca
     2641 caattccttt tacgtcctgt tcatctccgc tgattgtatg aaagatcact acgatcggca
     2701 tgatgctttt cttcttgttt ttcagaagag cgttctgttc gttccagcca gaccatgcca
     2761 ggccagtgat gcctccatcc ttcattcgaa gctggtctat tctgtcgaac ggatagtctt
     2821 tggatctgta ttccctgaac aataacagaa gaagcatcag gtgattgttg aagcaccagg
     2881 gcgtaggatg gtaggctcgg gtgaggacag ggacttctct caaaatctga tcatgaaatt
     2941 cattttcttg aaaatgcaaa actggtttat gagaaacgga gtagaagtag taggcacaga
     3001 gcagtgccag aaagcctacg cccgcgcaga tggtaagtac gatcggttcc agtgttttgc
     3061 tcctgaaatc cgaagtacag aatctacttc agcccacctc tcatttttta acttgcggtt
     3121 gttccgtcct caagtacgaa gggcatgtgt ccctggtgct cttgatcgat gcagatccaa
     3181 atcaagtctc gaagtttgag catgcaatgc aaggctctga agttcggctt cttacagcat
     3241 ccaatctagc tcggggtctc gagcttgcaa a
//
LOCUS       DAOQJL010000453         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19193_length_3265_cov_4.284424, whole genome shotgun sequence.
ACCESSION   DAOQJL010000453 DAOQJL010000000
VERSION     DAOQJL010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19193_length_3265_cov_4.284424"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLG78_16100"
     CDS             <1..318
                     /locus_tag="PLG78_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24696.1"
                     /translation="WIGSITAVPLSRATFSGQILTTLPDQSVIVYGKNAGRLLRDGMT
                     LEAREVDLLPLGRPAKKDQTTGTLKVISRPGDFIVAQWEGTPAPANAAFSFAPRPAKS
                     MFD"
     gene            complement(334..1425)
                     /locus_tag="PLG78_16105"
     CDS             complement(334..1425)
                     /locus_tag="PLG78_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24697.1"
                     /translation="MKPWLPLLLAVLYCAKPVEDPVPLVLIHGIKGSLLEDAEGQTAW
                     LSGREAMGWKHYRLSLPTEWKGEEQKRDGLRATGILKEVYVVPFLIGEKVYGPFIKGA
                     DKLDRPFYTFAYDWRRDNIENLRLFTAFLKEVSEKNRNAKVQVVAHSMGGLITRAALA
                     ENPELFHSVVFAGVPFSGGLGFLPDMHAGEPVGRNERILSPEVYFTFPSVYTLFQEGS
                     SRIYESGKELPINLYNADDWAKYKLGLFSLELVSPSQAAHLRMCLKRGKEFRRLASLP
                     VSKNIPVYVVLSKSTPTLATVERNGPKSVRGYDFESRPREPGDGRVREVDAFPPGLKA
                     ETLYIDKVHSELLNDTAVMDLVRKTGEKK"
     gene            1514..>3265
                     /locus_tag="PLG78_16110"
     CDS             1514..>3265
                     /locus_tag="PLG78_16110"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24698.1"
                     /translation="MRIGLILAIVLLNSCGAPPGMRSLNSTQINWEAKPGIAQPEDEG
                     FRPVDVPGNAASGSKEITYRLKFDMQPGMDSYGIRLGEISDRDRTYLNGTLIGSTGDW
                     NSDRPQAYDRTRLYTIPSGVLKSGQNILLVQVHGYFDYELGIYRDRVEIGPMASLLRE
                     YYLDNAGQALSIMVYVTIAAYFLLFFFRRRQDRENLYFALFLLSFILYSFFRTQFKYE
                     FGWPLFITKRIQFLTLFASVPLFFLFITSYFPLPEKPWATWFNRIAWLVMLIPAGAAV
                     MVLASPSGIVWQATLDYVQPTWIFFILGILFVVLRETFIHKDKDAMIMIGAILILVAA
                     LVVDTLAGRGRINLPTLLTHAFTLFVIAIALVLANRFVRLHDETEELNANLASLNRAA
                     QRFVPFEFLNILEKRSLLEVNLGDQVQREMTVVFSDIRGFTTLSEKMTPKENFDFINS
                     YLRRVGPSVREHGGFIDKYIGDAIMALFPVSPGEAVKSCIQMLGTVDRWNEKRVEFKY
                     EAVHVGFGIHTGRLMLGTIGENERMEGTVISDDVNLASRIESLTKMYGAEILISGAVL
                     DRIEKSEYKVRFADRVRV"
BASE COUNT          816 a          888 c          768 g          793 t
ORIGIN      
        1 tggattggaa gcataacagc agttccactt tcgcgggcca ctttttccgg ccagatattg
       61 accactctcc ccgaccagag cgtgatcgtt tatgggaaaa atgcagggag acttctgcgc
      121 gatggtatga ccctggaagc aagagaagtg gatttgcttc cgctcggaag accggcgaaa
      181 aaggatcaga ctaccggaac ccttaaggta atctcaagac ccggcgactt cattgtagcg
      241 caatgggaag ggacacctgc cccggccaat gcagctttca gctttgctcc gcggccggca
      301 aagagcatgt tcgactaaca atcgttatgc gatctacttc ttttcgcccg tttttcgaac
      361 cagatccatg acggccgtat cgttcaatag ttccgagtgc actttgtcga tgtagagcgt
      421 ttctgctttc aaacctggtg gaaacgcatc cacttcacgc actctcccgt ctcctggttc
      481 gcgcggtctc gattcaaaat catagccgcg cacactctta ggaccatttc tctcgactgt
      541 ggccagagtg ggcgtagatt tagagagaac cacatacacg ggaatatttt tggacacagg
      601 caaactggcc aggcgacgaa actctttgcc tctcttcaag cacatccgga ggtgagctgc
      661 ctgtgaaggg ctcacgagtt cgagcgaaaa aagcccgagc ttatactttg cccagtcatc
      721 cgcattgtag agattgatcg gaagctcttt tccggattcg taaatccgcg atgagccttc
      781 ctggaacagg gtatacacag aaggaaaggt aaagtacact tccggagata agatgcgctc
      841 atttctgccg accggttctc ccgcatgcat gtccggtaga aatccaaggc cccccgaaaa
      901 aggaactcca gcgaaaacga cggaatgaaa caactcagga ttttctgcga gtgccgccct
      961 tgtgatgaga ccacccatgc tatgagctac cacctgcacc ttcgcattcc gattcttttc
     1021 actcacctct tttaagaaag cagtgaaaag ccgtaggttt tctatgttgt cgcgtcgcca
     1081 gtcgtaggcg aacgtataaa acggacgatc gagcttatct gcacctttga taaaaggacc
     1141 gtagactttt tcaccgatca ggaaaggaac cacatacact tctttgagta tacccgtggc
     1201 ccggaggccg tctctcttct gctcttctcc tttccattca gtcggcaaag aaagacgata
     1261 gtgcttccac cccatcgcct cacgaccgct taaccaggct gtttggccct ccgcgtcctc
     1321 tagaagagaa cccttgatcc catgaatcaa cacaagaggc acaggatcct ccactggttt
     1381 tgcacaatat agaacagcta aaagtaaggg aagccacggc ttcatgcggt aatctggttg
     1441 tctcaggttc ccgattcaag tccttttcga caaaaaggat tgacccttcc gcgccgccgg
     1501 accatcctct ggcgtgcgaa ttggattgat cctggcaatc gtcctcctga attcttgcgg
     1561 tgctccgcct ggaatgcgaa gcctcaattc cactcagatc aactgggaag caaaaccagg
     1621 aatcgcacag ccagaagacg aaggctttcg cccggttgac gttccgggca atgcagccag
     1681 tggctccaaa gaaatcacat atcgactcaa attcgacatg cagcctggga tggattccta
     1741 tggcatccgc ctgggagaaa tctcagaccg cgacaggacc tatctcaacg ggaccttaat
     1801 cggtagcacc ggagattgga attccgacag gccacaggca tacgatcgca cacgtctcta
     1861 caccatccct tccggtgtac tgaaaagcgg ccagaacata ctccttgtgc aagtgcacgg
     1921 atatttcgac tacgagctcg gtatctatag agacagagtg gaaatcggac ccatggcttc
     1981 cctccttcga gagtattacc tggacaatgc aggccaggct ctatccatca tggtttatgt
     2041 gacgatcgcc gcgtattttc tcctcttctt cttcagaagg cgccaggata gagaaaatct
     2101 ttatttcgca ttgtttcttt tatctttcat tctttacagt ttctttcgca cacaattcaa
     2161 atacgaattc ggctggcctc tttttatcac aaaaagaatc cagtttttaa cgttattcgc
     2221 ctctgttccg ctcttcttcc tctttatcac gtcctatttc cctcttccgg aaaagccctg
     2281 ggccacatgg tttaatagaa tcgcctggct ggtcatgctc attcccgcag gcgcagcagt
     2341 catggttctc gcatcgccct caggcatagt ctggcaagcc actctagatt acgtacagcc
     2401 aacgtggatt ttcttcatac ttggaatcct cttcgttgtc ctcagggaaa ccttcattca
     2461 taaagacaaa gacgctatga tcatgatcgg cgcgattctg attctagtgg cagcactcgt
     2521 agttgacacc ctcgccgggc gcggccgcat caacttaccc actctgctca ctcacgcttt
     2581 cacgttattc gtcattgcta ttgcactggt tcttgcaaac cgatttgttc gcctccacga
     2641 cgaaacagaa gaactcaacg caaatctggc aagcctcaac cgggcagcac aaagatttgt
     2701 tccttttgaa ttcctgaaca tcctggaaaa gagaagcctc ctggaagtaa acctggggga
     2761 ccaggtgcaa agagaaatga ctgtggtttt ctctgacatt cgcgggttca cgactctctc
     2821 agagaagatg acccccaagg agaactttga tttcatcaac tcgtatctgc gccgcgtagg
     2881 ccccagcgtg cgcgaacatg gcggtttcat agataagtac attggtgacg ccatcatggc
     2941 acttttcccg gtcagcccgg gagaagccgt aaagagctgc atacagatgc tcggcactgt
     3001 agaccgctgg aacgaaaagc gagtcgaatt taagtatgaa gcggtgcacg ttggattcgg
     3061 gatccatacc ggaagactga tgctcggcac catcggcgaa aacgaacgta tggaaggaac
     3121 agtgatttcc gacgatgtga atctcgcatc gcgcattgag agcctcacca agatgtacgg
     3181 ggcggaaatt ctaatcagcg gtgcggtgct cgaccgaatc gaaaaatcag aatacaaagt
     3241 tcgcttcgca gatcgcgtgc gagtg
//
LOCUS       DAOQJL010000454         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19208_length_3264_cov_4.207541, whole genome shotgun sequence.
ACCESSION   DAOQJL010000454 DAOQJL010000000
VERSION     DAOQJL010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19208_length_3264_cov_4.207541"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLG78_16115"
     CDS             <1..1086
                     /locus_tag="PLG78_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE24699.1"
                     /translation="SGCPATEDMSCVQGFGFSNDLGFAGSSSYDLHPPEPFNPNLDRY
                     YIPVWAATSDAVPMSYQGAYTELSSPTGIVSDRQQFDLYVGLFYTIVHPDVQPHRVSN
                     QKKTVNGVDLYTPIDPDWLSPVIPIEARFYPQSQDDPPGSMYVIPHILTWTPHGEIDH
                     FRDYRNLVILNELVGNPSGVDGNAGAPELVEIYNRSSYNVDLYGWRISITGANSVDLG
                     ITSGTTCLSSTKLPPGGYLTLAYYVDSTQANLDPAADCDFSDGIARLKVPGVSGMTTG
                     TGSARLYSDASSTLVDYVQFTTAACSTSQTGLSSAVSAGIWTSGDCVAGNDYIRRLPN
                     ATETNRSTDWAGNTIPPGIPTPGAANN"
     gene            1096..2949
                     /locus_tag="PLG78_16120"
     CDS             1096..2949
                     /locus_tag="PLG78_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24700.1"
                     /translation="MKYFVLITFALLCAHGVAAQEKQPDQQKQVEQPAPPKNNKDAKD
                     LEQIKQTDVKMPSGTLTMQPSKPEPVKPTEKWLYEMNLFSLDLNEKKALLDRIQWKAS
                     FFTSFNYFNNSDLRVLNSTNQTSIEDTDDKLYFMLSGAEIDTFFPIHPRLDFRVGLFK
                     AGFWGHDQLGGRDANNDIKFTRSGANTVNFDYLYFDVHFLKEPTSTRRADVRVGRQGY
                     SIGGEIERDFYLDDTLDAIVFKADSKFGSLNLLLIDVYANGSNTSDVYSVQYLSFDDE
                     KVDGFDGDVNTYRYGFVYMFPFIRAQNSLKETHIEARAFYYFARFGGLNDGGSDRTNK
                     GTTGNFSDNDFSTMKGVRLNAGFRGYVRGQITYAESAGIDRKRPTVAFTSQDVDTNGQ
                     SWMLDVEVSLFKRIVLLYPSVYWSEGGKYFTDGRPYSHGFVGFKGDHAGGILSDLNWG
                     MYPSAYVDDDGIDDTPFERQRKSGTFIRHLGAAIGKPDVIYFRVDWWRMEDTNRVGLV
                     NGATPGISSILSTKYVGPDLYEQQIRVEILKYIYPTAATQLLAYRRFSTPMAEEMNFT
                     VDWNVLPNWRAWAVVGVLRPMRYYATAGLEQGVPQGTARFTGMQVGTSFYF"
     gene            2997..>3264
                     /locus_tag="PLG78_16125"
     CDS             2997..>3264
                     /locus_tag="PLG78_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24701.1"
                     /translation="MKRYLIVSVLFLAACKNTHDPLFGLGDPGYFLLGPNRLSVYFND
                     PGVDEQTMHDKKVDMRLVDLINDAKKTVDIAVYNLGRQTIIDALI"
BASE COUNT          821 a          788 c          831 g          824 t
ORIGIN      
        1 tcaggttgtc ctgcgacgga agatatgtcg tgcgtgcagg gttttggttt ttcaaacgat
       61 ctgggatttg cagggagttc cagttacgat ctccatccac cagagccgtt caatccaaat
      121 ctggaccgtt actacattcc ggtgtgggcc gccacaagcg atgctgtacc gatgagctat
      181 cagggggcat acacagagct gtccagtcca acaggaatcg tttcagacag acaacagttt
      241 gatttgtacg ttggtctttt ctataccata gttcatcctg acgtgcagcc tcaccgcgtc
      301 tccaatcaga aaaagacagt gaacggagta gatctttata cgcccatcga tccggactgg
      361 ctttcccctg tgattcccat tgaagccagg ttttatcctc aatcgcaaga cgatccgcca
      421 ggatccatgt atgtgattcc gcatattcta acctggactc ctcacggaga aatcgatcac
      481 tttcgcgact atcgcaacct ggtcatcctc aacgaacttg ttggaaatcc aagcggtgtg
      541 gatgggaacg caggtgctcc ggaacttgtg gaaatttaca atcgatccag ctataacgtg
      601 gatctctacg gctggcgtat ttccattaca ggcgcaaatt ccgtggatct gggaattact
      661 tccggcacta cctgcctttc ttctacgaag ctacctccgg gcggatactt gactcttgca
      721 tactatgtgg attccactca agccaatctg gaccctgctg cagactgcga tttctcagat
      781 ggaatcgcgc gtctgaaagt tcccggggtt tccggtatga ccactggaac cgggtcggcc
      841 aggctttatt ctgacgcaag ctccactctc gtggactacg ttcaattcac tacagctgcc
      901 tgcagcacgt cacagaccgg actctccagt gcagtctctg ctggaatctg gacatcgggg
      961 gattgtgtgg caggcaacga ctacatacgc agactcccca atgcaacaga gaccaatcga
     1021 tctacagact gggcgggaaa cacgattcca cccggaattc cgacgcccgg agcggctaac
     1081 aactaggaaa atgatttgaa gtattttgtt ttaatcacct ttgcattgtt atgcgctcac
     1141 ggggttgccg cgcaggaaaa gcaaccagat caacagaagc aagtcgagca gccggcgcca
     1201 ccgaagaaca ataaggatgc gaaggatctc gaacagatca aacaaacgga tgtgaagatg
     1261 ccatccggga cactgacgat gcagccgtcc aaaccggagc cggtaaaacc aacagagaag
     1321 tggctctatg agatgaacct gttttcgctc gatttaaatg agaaaaaggc gctgctggat
     1381 cgaattcagt ggaaagccag cttctttact tcctttaatt actttaataa ttcagacttg
     1441 cgggtgctta attccacaaa tcagaccagc attgaagata cggacgataa actctatttc
     1501 atgctttcgg gtgctgaaat tgatacattc tttcccattc accccaggct agattttaga
     1561 gtgggcttat ttaaggcagg cttctggggg catgaccagc taggcggacg cgatgcaaat
     1621 aatgacatca agttcacccg gagtggcgca aacactgtca attttgatta tctctatttt
     1681 gacgttcatt ttctcaaaga gcctacatcc acgcgaagag cggacgtgcg ggtcgggcgt
     1741 cagggttact cgataggcgg agaaattgag cgcgacttct atctcgatga tactctggac
     1801 gcaatcgtct ttaaagcgga cagtaaattc ggatctctca atcttctctt gatcgatgta
     1861 tatgcgaatg gatcgaacac atcggacgtg tattctgttc agtatttgag tttcgatgat
     1921 gagaaggtgg acgggtttga tggagatgtg aacacctatc gttacggatt cgtgtatatg
     1981 tttccattca ttcgcgctca aaattctctc aaggaaacgc acatagaagc gcgcgctttt
     2041 tattacttcg cccggtttgg cggactcaac gatggcggtt cagatagaac caacaaggga
     2101 actacaggaa atttctcaga caatgatttc tccacaatga aaggagtgag actcaacgca
     2161 gggtttcgcg gctacgttcg aggacagatc acatacgcag agagtgcggg gattgatcgc
     2221 aagcgtccaa cagttgcctt cacaagtcag gatgtggaca cgaacggaca gtcctggatg
     2281 ctggatgtgg aggtttcgct cttcaagcga atcgttctcc tttatccttc cgtctactgg
     2341 tcagaaggtg gaaagtattt cacggacggc aggccctatt cgcacggctt cgttggattc
     2401 aagggagatc acgcaggagg cattctgagc gacctgaact ggggtatgta tccctccgct
     2461 tacgtagacg acgatggcat tgatgacaca ccttttgaaa gacagcgcaa atccggaact
     2521 ttcattcgtc acctgggagc tgccattgga aagccagacg taatttattt tcgcgtagac
     2581 tggtggagaa tggaagacac caatcgagtg gggcttgtca acggggctac tcctggaatt
     2641 agttcgattc tctcaactaa atacgttggc ccggatttgt atgaacaaca gattcgcgtg
     2701 gagattttga aatacatcta tcctacggca gccacgcagc ttctggccta caggcgattt
     2761 agcacgccca tggccgagga aatgaatttt actgtcgact ggaatgttct gccaaactgg
     2821 cgtgcctggg ctgttgtggg cgttcttcgc cccatgaggt actacgcgac ggcgggtctc
     2881 gagcagggcg ttcctcaggg aacggctcgt tttacaggga tgcaagtggg tacgagcttc
     2941 tatttttgat tggcaatggc gatgggggat gtctgaatga gacggggggc gtgcgaatga
     3001 aacggtatct gatcgtttct gttttatttc ttgctgcgtg taaaaacacg cacgatccgc
     3061 tctttggcct cggagacccc ggatactttt tacttggccc caatagactt tctgtatatt
     3121 tcaacgatcc cggagtggat gagcagacca tgcacgacaa gaaagtcgac atgcgtctcg
     3181 tggatttgat caacgacgcc aaaaaaaccg ttgatatagc cgtgtacaat ctgggccgcc
     3241 agacaatcat tgatgcactc attc
//
LOCUS       DAOQJL010000455         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19246_length_3260_cov_4.925117, whole genome shotgun sequence.
ACCESSION   DAOQJL010000455 DAOQJL010000000
VERSION     DAOQJL010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19246_length_3260_cov_4.925117"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLG78_16130"
     CDS             <1..784
                     /locus_tag="PLG78_16130"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNE24702.1"
                     /translation="VPVESSKGEWGPGQQELNFRYCDVLGMCDRHTIYKQGIKEIAWA
                     QNLAVTFMAKWKTEYAGSSMHLHFSLWDKDIKKNVFAGDKKFGPVMCSDEFRWFLGGW
                     MQHIRAIMPFYAPYPTSYKRYLSQSWAPVYIAWSHDNRTAGFRVVGSGNSLRIEARFP
                     GADANPYLAFAASIAAGLDGLKNKIEPPQIFEGDVYSARELPRVPSNLMEAVSHLEKS
                     EMLKEAFGADVVEHYVHFFKVEQEKFDQQVTTWERGRYFERA"
     gene            complement(781..1788)
                     /locus_tag="PLG78_16135"
     CDS             complement(781..1788)
                     /locus_tag="PLG78_16135"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24703.1"
                     /translation="MKGLASIWFLLVLAGCHRDDCLVYDGECNPFVTALLFSRVQAAR
                     FIAVSNSTGGSVSMYQMNYSTGTLLPVSGSPFAAGASPHHLAAHPSGRFLYLANNGSN
                     NISGYAVNSNTGALTQVSGSPFASVTAPYSVTIDPTGTYLYAGSETTAFISAYQIDSS
                     TGVLTQISGSPFGASPVAGSVRVSPSGRFLYVGVNNATGQVLAYAIASNGSLTTISGS
                     PFASGNDTISVTVDPYERFVLGINYFGDATGLVALSAYFIGASGAMIPLGAGPYAAGA
                     SPIGCAAEPLGRYVFSANTGSNTVTMHTINQTSGVPTIVGTYSTGNGPFGVSFIPTLQ
                     Y"
     gene            1929..3227
                     /locus_tag="PLG78_16140"
     CDS             1929..3227
                     /locus_tag="PLG78_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24704.1"
                     /translation="MARRIGLLLLLSLCPLSAEIVQLRDGRTMRGRITAQDTETISLR
                     TQDGDYLIYRDQVYRILLDDPAVLRAQEAAEAEKEARVTPQTQVRDQIREETKVPESP
                     GKRPETPGKRDEKKEEKHANFLFGLSAGGAKHDSVYKEIFVDDKTADYVQQGIALTSS
                     VSYPYEFSESKLKTYAAYMYYESGFWAVGAEGETGSHSSSYQLMESLTNSGRFYAGAG
                     SFTKTTDQIYRLRAGILAGSESWTVQPWIFAGPVFLASRSKGNQTEVLTNPTTLAAAA
                     VYSPSLQYKSSAQGLEAATEIRWLISGGMELRGKGAFGQMKGGLHYDEFRLPLQDATA
                     YAALTAYPTWVIQSAQLRTVLTQYGAAFYFPLGDMFRVFVDLGMTRVQYKLSNLGTLR
                     LPDNALAPGATANGDLYRYIFGKSFNDTSRAYRVGVEFRL"
BASE COUNT          929 a          823 c          836 g          672 t
ORIGIN      
        1 cgtgcccgta gaatccagca agggagaatg gggccccggg cagcaggaac tgaacttcag
       61 atattgcgac gtactgggca tgtgcgatcg ccacacaatt tacaaacagg gaatcaaaga
      121 gatcgcctgg gcgcagaacc tggcagtaac cttcatggca aaatggaaaa cggaatatgc
      181 cgggtccagc atgcaccttc atttttcgct gtgggacaaa gacataaaga agaacgtttt
      241 cgcgggagat aagaaattcg gacctgtgat gtgctctgac gaatttcgct ggtttctggg
      301 cggttggatg cagcatatcc gcgccatcat gcccttctac gcgccctatc caacttctta
      361 caaaagatat ctatctcagt cctgggcacc cgtttacatt gcatggagtc acgacaatcg
      421 cactgcaggc tttcgcgtag tgggatcagg aaattctctc agaatagaag caagatttcc
      481 aggtgccgat gcaaatccat atcttgcgtt tgctgcatcc atcgcagcag gcctggatgg
      541 actcaaaaac aaaatagaac ctccgcaaat ctttgaagga gatgtttact cagcgcgaga
      601 actcccacgt gtaccttcca atttaatgga agcagtttcg cacctcgaga aatctgaaat
      661 gctcaaggaa gcattcgggg cagacgtggt ggaacactac gttcatttct tcaaagtaga
      721 acaggagaaa tttgatcagc aagtaaccac ctgggaacga ggaagatact tcgagcgcgc
      781 ctagtactgc agggttggaa taaaggagac accgaatgga ccgtttccag tggagtaagt
      841 ccctacaatg gtgggaactc cactcgtctg gttgatcgtg tgcatggtta cagtgtttga
      901 gccggtgttt gcagaaaaga cgtatcgacc gagtggttcg gcggcacagc caatcgggct
      961 tgctccagct gcataggggc cagcgcccag cggaatcatg gccccgctcg caccaataaa
     1021 atacgcggac aaagccacga gtcctgtggc gtctccaaaa tagttaattc ctaaaacaaa
     1081 tctttcgtac ggatctaccg tgaccgaaat cgtatcattg ccagaagcaa aaggggatcc
     1141 ggaaatcgtg gtcagggaac cgttagacgc tattgcgtaa gccagaacct ggcctgttgc
     1201 attgttgact ccaacgtaaa ggaaacgacc ggatggagaa acacgcacag atccggcaac
     1261 gggactggca ccaaaaggag atccggaaat ctgagtcaag actccagtag aactatcaat
     1321 ctgataggcg gaaataaacg cagtggtttc cgatcctgca taaaggtagg ttcctgttgg
     1381 atcaatcgta acagaatagg gagcagtcac ggaagcaaaa ggagaaccag aaacctgtgt
     1441 cagagcaccc gtgttcgaat tcacagcgta cccggatatg ttattggaac cattgtttgc
     1501 aagatacaaa aatcggcccg aaggatgggc cgctagatga tgagggctcg cacctgcagc
     1561 aaacggagat ccggagacag gcaagagcgt tccagtggag tagttcattt gatacatcga
     1621 aacgcttccg cccgtactgt tcgagacggc gatgaacctg gccgcctgaa cacgcgaaaa
     1681 taacaaggcg gttacgaagg gattgcattc gccatcatag accagacaat catcgcgatg
     1741 acaaccggcc agaaccaaca gaaaccagat ggatgctaag cctttcatga tggggaaccc
     1801 atacaaagaa acgcactacg cgcaggaaac aaccagaatc catcgcataa cccgccgaag
     1861 acctaaaaac ccttaaaata aaaggggagt ccacgaaatt tccgtccaat gaatgccgaa
     1921 ggtaccctat ggcccgccgc attggcctat tgctactgtt gtccttgtgc cctctgagtg
     1981 cagaaatcgt gcagctccgg gatggtagaa ccatgcgagg ccgaatcacg gcccaggaca
     2041 cagaaaccat atcactgcgc acccaggatg gggattatct aatttatcgc gatcaggttt
     2101 atagaattct cctggacgat ccggcggtgc tacgggccca ggaagcagca gaagcagaaa
     2161 aagaagcaag ggtcacaccc cagacccagg tacgcgatca aattcgcgaa gaaacgaaag
     2221 tgccagaatc ccccggcaag cgcccggaaa ctccgggcaa acgagatgag aagaaagaag
     2281 aaaagcatgc gaactttctc ttcggactct ctgcaggagg ggcaaagcac gactccgtat
     2341 ataaagaaat cttcgtagac gataaaactg cggattatgt ccagcaggga atcgcactca
     2401 ccagttccgt atcctatcct tacgagttca gtgaatcaaa actgaaaaca tacgcggcat
     2461 acatgtatta tgaatccgga ttctgggcag taggagcaga aggcgaaacc gggtcacaca
     2521 gttcaagcta ccagctcatg gagtcactga cgaatagcgg tcggttctat gcaggcgccg
     2581 gatcctttac aaagacaaca gatcaaatct accgccttcg cgcgggcatc cttgcaggaa
     2641 gcgaatcgtg gacagttcaa ccctggatct tcgcgggccc tgtatttctc gcaagcagat
     2701 ccaaaggaaa tcaaacagaa gtactcacca atccaactac tctggcggct gccgcggtgt
     2761 acagtccatc tctgcaatac aagtccagcg cgcagggtct cgaagccgcc acagaaattc
     2821 gctggctcat ctctggagga atggaactga gaggcaaggg tgctttcggt caaatgaagg
     2881 gtggactcca ctatgatgag tttcgcctgc cgttacagga tgcaaccgct tatgcggcgc
     2941 tcactgccta cccgacctgg gtgatccaga gcgcacaact tcgcacagtt ctcactcagt
     3001 acggtgctgc gttttacttc ccgttaggcg acatgttccg agtgttcgtc gatctgggca
     3061 tgacgcgagt tcagtacaaa ttgtccaatc tgggtactct tcgccttccg gacaatgcac
     3121 ttgcacccgg agccacagcc aacggggatc tttacagata tatttttgga aaaagtttca
     3181 acgacacaag tcgcgcttat cgcgtgggtg tagaattcag actctgatct ctcacgaggc
     3241 ttcgaaatac attttcgaga
//
LOCUS       DAOQJL010000456         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19253_length_3259_cov_4.479089, whole genome shotgun sequence.
ACCESSION   DAOQJL010000456 DAOQJL010000000
VERSION     DAOQJL010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19253_length_3259_cov_4.479089"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLG78_16145"
     CDS             <1..1910
                     /locus_tag="PLG78_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNE24705.1"
                     /translation="ENLYAPENVELVAHTTQALRAHLVYKHEVDYWVREGEVMIVDEH
                     TGRLMEGRRYSDGLHQAIEAKEGVEIRQETQTLASITFQNFFRIYNKLAGMTGTADTE
                     SEEFKKIYRLEVVVIPTNVRVQRIDHPDRVYRSEREKFNAIVQELAALHAKRQPVLVG
                     TISIEKSEMLSGLLKKAGIPHSVLNAKHHEREAEIVENAGVPGAVTVATNMAGRGTDI
                     VLGGAARYLKDLEELEETDEASAKFRNAVLKKEFAEARNLISTLDTAARKDLAKAIAD
                     RAEIWLKSHKEVKELGGLHILGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDQL
                     MRLFGGQRLMNIMERLGMTEGQELEAGMVDRAIARAQKRVENHNFDMRKHLLEYDDVM
                     NRQREFIYAERNKLMSNENVRAHLREWMNEVIENKVLEFCEGSDPFRWDLESLKEWTR
                     TVLGLDLNLDADDYKRVRNPLETLYDHIVEKSIEHYDAHAASIGGENFSYIERRIALD
                     VIDARWKDHLYAMDHLREGIWAISYSERNPLVEYKIQGFRLFDALVQLIKEQITEFLF
                     RVEVQGPIQQTSRRQPVGQEQHQSLGSFESTSGPGVGPGGLPAASQKAPEITVSGGGA
                     SRRKSSRKRRR"
     gene            1922..2647
                     /locus_tag="PLG78_16150"
     CDS             1922..2647
                     /locus_tag="PLG78_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE24706.1"
                     /translation="MSSPEEFLRKLEAQKLRFVIALSSVVFPVTVIQSIIRWNAGLKL
                     PALINCVLMVGALISLVLALKRKLNPAAHALALLASITTGLNQFVTSSGTIAPLLNVL
                     VLFAASVYMLPLKWGIFYGVLAFVRAITIRLLSDVWGIRGDSPIVPVTEWYTAMFVSF
                     FVFFYVMVARETSAHRLLDTYRQAAQTRARFLSRMSHEIRTPLNGVLGIGEVLGEKLH
                     EPEQKEYLRIMMNSGRHLLHIVN"
     gene            2648..>3259
                     /locus_tag="PLG78_16155"
     CDS             2648..>3259
                     /locus_tag="PLG78_16155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24707.1"
                     /translation="MLELARTENRITLVRENYEPALVLNQVAELFRSALTSKDLGVHI
                     EVDPALPRVVSGDEGSVRQILVNLMHNAVKFTEKGGISLRVRTDASGGLERIIYSVED
                     SGRGISAEKVTSVFDAFEQGDVTTATADGTGLGLAICRELCAQMDGVIELRSKKSEGS
                     AFIVSLPLFMPVTGVVPVESKTGIKSFPEGSVAVLYVEDDGVNR"
BASE COUNT          827 a          758 c          901 g          773 t
ORIGIN      
        1 tagagaatct gtacgctccg gaaaacgtgg agcttgtggc gcatacgact caggcgctgc
       61 gtgctcatct tgtatacaag catgaagtgg actactgggt tcgcgaaggc gaggtgatga
      121 tcgtcgatga gcacactggg cgtctcatgg aaggccgccg gtactcagac ggtttgcatc
      181 aggccataga ggccaaagaa ggcgtggaga ttcgtcagga aactcagacg ctggcttcta
      241 ttacttttca gaacttcttt cgcatctata acaaacttgc aggcatgacc ggtactgcgg
      301 atacggaatc agaggaattc aagaagatct atcgcctgga agtcgttgta attccgacta
      361 acgttcgcgt gcagagaatt gatcatccgg atcgggtcta tagaagtgaa cgtgaaaaat
      421 tcaatgcaat cgttcaggaa ctggcggccc ttcatgccaa gcgtcaacca gttctggttg
      481 gaacaatttc gattgaaaaa tccgagatgc tttctggact tctgaagaag gctggtatcc
      541 cgcacagtgt cctcaacgca aagcaccacg agcgcgaagc tgagattgta gaaaacgccg
      601 gtgtgccggg agcggtcact gtggccacaa acatggcggg tcgcggaacc gacattgttc
      661 ttggtggtgc tgcgcgctat ctcaaagatc tcgaagagct agaagaaaca gatgaagcat
      721 ctgcaaagtt tagaaacgca gttttaaaga aagaattcgc ggaagcgcga aatctcattt
      781 ccacactgga cacagctgct cgcaaagatc tcgccaaggc cattgcagat cgcgctgaaa
      841 tctggctgaa aagtcacaag gaagtaaaag aactgggtgg ccttcatatt ctgggaacgg
      901 aaagacatga agctcgcagg attgacaacc agttgcgcgg tcgttctggc agacaggggg
      961 atccaggatc cagtcgtttc tatctcagtc tagaagatca gctgatgcgc ctctttggcg
     1021 gacagcgtct gatgaatatc atggaacgtc tgggcatgac cgaagggcag gagctcgaag
     1081 caggcatggt ggatcgcgcg attgcccgcg cacagaagcg tgtggaaaat cacaactttg
     1141 acatgcgtaa acatcttctg gaatacgatg atgtgatgaa tcgccagcgc gaattcattt
     1201 acgcggaacg caacaagctc atgagcaacg agaatgttcg cgcgcatttg cgggaatgga
     1261 tgaacgaagt cattgagaac aaagttcttg agttctgcga aggatcggat ccctttagat
     1321 gggatctgga gagcctgaaa gaatggacgc gcacggtgct tggcctggat ctgaatcttg
     1381 acgcggatga ctacaaacgc gttcgcaatc ctctggaaac cctctacgat catatcgtgg
     1441 agaaatctat cgagcactac gatgcccacg cagcatcgat tggcggagaa aactttagct
     1501 acattgaaag aagaatcgcc ctcgatgtaa ttgatgcgcg ctggaaagat cacctctatg
     1561 cgatggatca ccttcgcgaa ggcatctggg ccatttcgta ttctgaaaga aatcctctcg
     1621 tagaatacaa gattcaggga ttccgtctct ttgatgcgct cgttcaattg atcaaagaac
     1681 aaatcactga attcctgttt cgcgtggaag ttcaggggcc catccagcag accagcaggc
     1741 gtcagccggt gggtcaggaa cagcatcagt ctctgggtag ctttgaaagc acttcgggcc
     1801 cgggtgtagg cccgggaggc cttcccgctg cgtcgcagaa agctccagag atcaccgttt
     1861 caggcggtgg cgcgtccaga aggaaatcgt caagaaaacg gcggcgttag gcggcattga
     1921 tatgtcgagt ccggaagagt ttctgcgcaa actcgaagcg caaaagctgc gctttgtcat
     1981 tgcgctatct tccgttgttt ttccggtcac tgtaatccag agtattatac gctggaatgc
     2041 cggcttgaag ttgcccgctc tcatcaactg cgtgctcatg gtgggtgcat tgatttcact
     2101 tgtgcttgca ctgaaacgga agctgaatcc agcagcgcat gcactggcac tccttgcatc
     2161 gatcacaacc ggtctcaatc aatttgttac ttcctccggg acaattgctc cgctgctgaa
     2221 tgtcctcgtt ctatttgcgg cctctgtcta catgctaccg ctcaaatggg gcatattcta
     2281 cggagtccta gcttttgttc gagccatcac catcaggctt ttgtctgacg tatggggcat
     2341 tcgaggagat agccccattg tccctgtgac tgaatggtat acagccatgt tcgtttcttt
     2401 ctttgttttc ttctacgtta tggtagcccg cgagacaagc gctcacagac tccttgatac
     2461 atatcgccag gcagcgcaga cacgcgctcg ctttctatcc agaatgagtc acgagattcg
     2521 cacgccgctc aatggcgttc ttgggatcgg agaagtcctc ggagaaaaac ttcatgagcc
     2581 agagcagaaa gaatatcttc gcatcatgat gaattccggt cggcatttgc tgcacattgt
     2641 aaattaggtg ctcgagctgg cgcgtacgga aaatcgcatc actctcgtgc gagaaaatta
     2701 tgagccggcc ctggtactga atcaagtagc ggagctattt cgaagtgcgc tgacgagcaa
     2761 ggacctcgga gtccatattg aagtggatcc agctttgcct cgagtcgtgt caggagacga
     2821 gggaagcgtg cggcaaattc tggtgaatct aatgcacaat gcagtgaagt ttaccgaaaa
     2881 aggtggaatc tctctgcgag tgcgaacgga tgcatccgga gggttggaaa gaattatcta
     2941 ttcagtagaa gacagcggcc gtggaatcag tgcggaaaaa gtgacttctg tgtttgatgc
     3001 gtttgaacag ggagacgtca caacagcaac ggcggacggg acaggtcttg gccttgcgat
     3061 ttgtcgtgaa ctctgtgcgc aaatggacgg cgtcattgag ctgcgcagta agaagtcgga
     3121 gggtagcgca ttcatagtga gtttgcctct cttcatgcct gttactggcg tagttccagt
     3181 ggagtcaaag accggtatta agagttttcc ggaaggctct gttgctgttt tgtatgtaga
     3241 agacgacggc gtgaacaga
//
LOCUS       DAOQJL010000457         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19329_length_3251_cov_5.364518, whole genome shotgun sequence.
ACCESSION   DAOQJL010000457 DAOQJL010000000
VERSION     DAOQJL010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19329_length_3251_cov_5.364518"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLG78_16160"
     CDS             complement(<1..1118)
                     /locus_tag="PLG78_16160"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNE24708.1"
                     /translation="MTEQRTLQYVTASDKIHYLVTNHVSTRSLFVKGSGDPPLEVTNR
                     GFNVENNVLMVENKKLQLSLEQEVTLFRILGRYMELNCVVVANSSPGVYQLHVKTAGI
                     APKDRGNLRIPVNPELVVITNIRASKHAIDASLFSIPTTVKVSFADYERRLKAQEDYV
                     KIDVYGAKGTIFDEIRKTNRTLFMEDTQNPACYKAPSPFYIDYDSLLFEGIQKRMQEY
                     KREKVQSEVIVPVTYITHDMTSIPLGYIHMQSKTKKYSEEKVLELQQLAFEMVDRIRD
                     SNTVIMTHREEVVNLSRGGLKVLIKNPELQEHLRRQSGFTFDVLFRMQGPITLFGKVR
                     SAVQDHEGHLLIGIQISGTSSRENEMKRYLDNVSQLEK"
     gene            1418..2719
                     /locus_tag="PLG78_16165"
     CDS             1418..2719
                     /locus_tag="PLG78_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24709.1"
                     /translation="MKQFLKWTGGTLLVASMPLMAADPPAATPATPAAPAAPALPNGG
                     VDVSLQLSSNYVFRGADMYQNKFVQDRQSYGKWNKAPSFQPSVTFKTPVDGLTFNIWG
                     AFALTNRNDKDVDQRIQNGKGGVTSNDTILANPLIDSIDYNSSATPPNTVGTSPSQYL
                     RNYFNTASTFYPTGNYAGANTVNVPGFYKDANGLSRVDELDYTIGYSSATKVGTIGFG
                     LITYQLANAKGKGNPFIAGGSAFYYATEMFASYALPMLPDLTAKAFADIVNANQYYQL
                     VYSKTIAVSDAVSVTVTTGPSYSIQSALYAVPNGTANTYAGTTQTLQGWKDVTSSVGV
                     SAKGFNVQLNAAYRPDLRFYDSDPSTNRLVEIDGGSTMNDGRVADPKWTGSGVAADVV
                     QRGITAQGRRLAAGNSNYQFVARQKLPRWIYWVNAGYTASF"
     gene            complement(2783..>3251)
                     /locus_tag="PLG78_16170"
     CDS             complement(2783..>3251)
                     /locus_tag="PLG78_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24710.1"
                     /translation="GCTMGVVLSRNNAGGLFPIFSLIAAPSTDGRMLGFNPSNQICLH
                     DSQGAPSGLLFTGSFPASSGPSILIYTGSGTTGNFYVNGILALSGTVASGACPVTAPT
                     SSVAMGTKNGGGSYYDGRIAEFFYSESSITSAQAASLSCYYKNKFSVTAATCL"
BASE COUNT          867 a          823 c          800 g          761 t
ORIGIN      
        1 ttcttctcaa gctgagaaac gttgtcgagg taacgcttca tttcgttttc gcgtgaggat
       61 gtaccggaga tctgaatccc aatcaaaaga tgtccttcgt gatcttgaac agcagatctc
      121 actttcccga acaatgtgat cgggccctgc atgcgaaaca aaacatcaaa cgtaaaaccg
      181 gactgcctgc gcaaatgctc ctgtaactcc ggattcttga tcaaaacctt gaggccaccg
      241 cgagaaagat tcacaacttc ttctctgtga gtcataatta cagtgtttga atcacgaatg
      301 cgatccacca tttcaaacgc aagctgctga agctcgagaa ccttctcttc agaatatttc
      361 tttgtcttgc tttgcatgtg gatgtagcca agcggaatcg aagtcatatc gtgcgttatg
      421 tacgtgacag gcacgatgac ctctgattgg actttttccc ttttgtactc ctgcattctt
      481 ttttgaatgc cttcaaacaa aagagaatcg taatcaatat agaaagggct gggagcctta
      541 tagcaggccg gattctgcgt atcttccatg aagagagtgc gatttgtctt tcgaatctcg
      601 tcaaagatcg taccttttgc tccataaacg tcgattttca catagtcttc ctgcgccttc
      661 agcctgcgct cgtagtccgc gaagctcact ttgactgtgg taggtatgct aaaaagactc
      721 gcgtcgatcg catgcttgct tgctcgaatg ttggtgatca ctacaagctc cggattcaca
      781 gggatacgca ggttcccgcg atccttggga gcaatccccg cagtcttcac gtgaagctgg
      841 taaacgcccg ggctggaatt ggccacaacc acgcaattga gctccatgta acggccgagt
      901 atgcgaaaga gagtcacctc ctgctcgagc gaaagctgca gcttcttatt ctcaaccatg
      961 agcacattgt tttcgacgtt gaagccgcga ttggtaactt cgagaggagg atcgccactg
     1021 cccttcacaa agaggcttcg cgtggacacg tggttggtga ccaggtagtg gatcttgtca
     1081 gaagccgtaa cgtattgaag agtgcgctgc tcagtcatcc aaaggttgga tggcagaggc
     1141 cgaatctgcg gcaaacacta tttcgcgcgc gatttgtgca ccgtggaagc gcagaaaaat
     1201 agccatgcga cataatcact tcggtgagca gtagatccgg aggcgggagc ttactgaaat
     1261 ctggccaatg cctaaaaaaa gggcaatctg cccggctctt tttgaccgaa gataggacag
     1321 gtcattcctg aatgcaccga attggggcag aatgtggtca tttccatggc acgctttgtg
     1381 caaaagtggg ttcaggtata aaatccggag gtttccgatg aaacaatttc tcaaatggac
     1441 tggaggaacc ctgctggttg cttccatgcc actgatggct gcagatccac cagcagccac
     1501 accagccacg ccagcagcgc cggcggcacc tgctctgcca aatggcggag tcgatgtgag
     1561 cctgcaactg tcgtcgaact acgtattccg tggcgcagac atgtatcaga acaagttcgt
     1621 ccaggacaga cagtcctatg gcaagtggaa caaggcgcca tcgtttcagc cttctgtcac
     1681 tttcaagact cctgtagacg gtcttacttt caacatctgg ggagctttcg ctctcacaaa
     1741 cagaaatgac aaagacgtgg atcagcgtat ccagaacggg aaaggtggcg tgacttcaaa
     1801 tgacacgatc cttgccaacc ctctgattga ctcgatcgac tacaacagct cggcaactcc
     1861 gccaaacact gtgggaacat caccctctca gtatctgcgt aactacttca acacagcttc
     1921 cacgttctac ccgaccggta actatgccgg tgcaaacaca gtcaatgtgc ctgggtttta
     1981 caaagatgca aacggcttga gccgagtgga tgaactggat tacacgattg gttattcaag
     2041 cgcaaccaaa gtgggaacga taggttttgg cctcattact taccagctgg ccaatgcgaa
     2101 aggaaaagga aacccattca tcgcgggtgg ctctgccttc tactacgcaa ctgaaatgtt
     2161 cgcaagttat gctctgccta tgctgccaga tttgacagca aaagcatttg ctgacattgt
     2221 aaacgcaaac cagtactatc agctcgtcta ctctaagacg attgcagtga gtgatgcagt
     2281 atctgtaaca gttacaaccg gtccaagtta cagcatccag agcgcactgt atgccgttcc
     2341 aaacggaacc gcaaacacat acgcaggcac aactcagact ctgcagggct ggaaagacgt
     2401 gacttcttct gtgggtgtgt ccgcaaaagg attcaacgtt cagctcaatg ccgcctatcg
     2461 tccagacctt cgcttctatg acagcgatcc atccacaaac cgtctggttg aaatagacgg
     2521 tggctcgaca atgaacgatg gtcgcgttgc agatccaaaa tggactggtt caggtgtagc
     2581 agctgacgtt gttcagagag gtatcacagc ccagggacgc agactggcag caggcaactc
     2641 aaactatcag tttgttgcac gccagaaact tcctcgctgg atttactggg taaacgctgg
     2701 ttacacagct tccttctaag aaggaaagat cttgagcaaa gagaagccgc cccgcaaagg
     2761 gcggcttttt ttgtcccatc attcaaaggc aggttgcggc cgttacggaa aacttgttct
     2821 tataatagca ggagagtgaa gccgcctgag cactggtgat gctagactct gaataaaaga
     2881 actccgcgat tcttccatca tagtaacttc caccaccatt cttcgtaccc atggccacag
     2941 aggaagtggg agcagtgaca ggacaggcgc ccgatgcaac tgtaccgctc agtgcaagaa
     3001 taccattcac gtagaaattc cccgtcgttc cagaaccagt atagatcaga atgctcggtc
     3061 cggaagaagc aggaaaagat ccagtgaaca gaagacccga aggcgcaccc tgagaatcat
     3121 gcaagcaaat ctggttggag ggattgaatc cgagcattcg cccatctgtg gatggagcag
     3181 cgatgagaga gaaaatcgga aagagaccgc cggcattgtt gcgcgaaaga acgactccca
     3241 tggtacatcc g
//
LOCUS       DAOQJL010000458         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19669_length_3216_cov_4.462828, whole genome shotgun sequence.
ACCESSION   DAOQJL010000458 DAOQJL010000000
VERSION     DAOQJL010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_3216_cov_4.462828"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PLG78_16175"
     CDS             <1..1104
                     /locus_tag="PLG78_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24711.1"
                     /translation="EEEARRLEAERVAREEAARRQNADVTKQKEAEQERKKKEKEADA
                     AREAGMRRRFAIVGASAVRGFYQAAFQEQYTQHVLMTVQRGGIFPGAVFTSDLHDRDL
                     KGGEAFGSLTWNRLYFRGSYLSFHAQPKLIQGLMPSSQSGPVLTRDDLTMDLKRSEAG
                     AAAGYSFVRTPTFELRALALFRDEIMNANIRSAGASVSTDSTRPFYGDFNYDDGVRHR
                     SYGHGGGLSAIYNVGKLKLSIDLESYRLHGPWGLKYKKTERSNGSPGFAPLYVDTEGT
                     IRKTTSVLSPAAGYEVFAGFSIRLAARFEQASYQIRRATMPLFLGGASPPDFASALFL
                     QNVVYNGFGFSEKLNRHDSVQYVSLGIEKKIDF"
     gene            complement(1101..1595)
                     /locus_tag="PLG78_16180"
     CDS             complement(1101..1595)
                     /locus_tag="PLG78_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24712.1"
                     /translation="MRFLENRVVLPTSIPGELKLPAKSALMAHMDRKVESCEKIDDRI
                     TFRGKMYGAGRNPWANCDGEITLSTPGKFILITASLSHLRAAFWQLLFYPALGSLTLT
                     LILRYFFGRPSWMVPAIIAALFCIGGIRTIRRAPGRFREAMIDSLRSLPRDSSSWNDE
                     LAGI"
     gene            1711..2184
                     /locus_tag="PLG78_16185"
     CDS             1711..2184
                     /locus_tag="PLG78_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24713.1"
                     /translation="MIFLSCSGAPVQEGAIRGSAGGYTIVFPQKWKIVRPDDVTLEAV
                     SEDELAGVRIVEQGRIGKSAEDILGKIEKQLEVQNSLPESSRTLDANLLKESNAQHCA
                     IGQYALRKNGVLLRRKIWVFVKGQRAFVITTNVPESYAGEYPGVFEGIIKSLRAQ"
     gene            complement(2189..>3216)
                     /locus_tag="PLG78_16190"
     CDS             complement(2189..>3216)
                     /locus_tag="PLG78_16190"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNE24714.1"
                     /translation="FSGFKMKFDAMHAVTGPYAVEILQKRLGASAGTVMNAVPLEDFG
                     GGHPDPNLTYAHDLVEIAYGKDGPDFAAASDGDGDRNMILGKGFFVNPSDSLAVLAAN
                     ATLVPAYKKGLAGIARSMPTSAAADRVAQSLGLACFETPTGWKFFGNLMDAGKVTLCG
                     EESFGTGSNHVREKDGLWAVLFWLNILAVKHQSVETVMREHWNKFGRNFYSRHDYEGI
                     PSEAASGLMKHVAESFAELTGKKFGKHEIRFCDDFSYNDPVDASVSSGQGIRIIFVDG
                     SRIIFRLSGTGTEGATLRLYLEAYEPDPARHNLDAQEALSDFITIASQISGLRERTGR
                     ERPTVIT"
BASE COUNT          773 a          802 c          828 g          813 t
ORIGIN      
        1 gaagaggaag cgcgtagact ggaagcggag cgcgtggcac gcgaagaagc agccagacgc
       61 cagaacgcgg acgtcacaaa acaaaaagaa gcagagcaag agcgaaagaa gaaagaaaaa
      121 gaggcggatg cagcgcgcga agctggcatg cgcagacgat ttgccatcgt gggagcctct
      181 gccgttcgcg gattctacca ggcagctttt caagagcagt acacgcagca cgtcctcatg
      241 acagtgcaga gaggtgggat ttttccagga gcagtcttta cttctgattt acacgaccgg
      301 gacttaaaag ggggagaagc atttggatct ctcacctgga atcgtcttta ttttcgcgga
      361 agctatcttt cttttcacgc acagccaaag ttgattcagg ggctgatgcc ttcgagtcag
      421 tctggtccgg ttctgactcg tgacgatttg actatggact tgaagagaag cgaagcgggt
      481 gcggccgcag gatattcttt cgttcgcaca cccacttttg aacttcgcgc cctggctctc
      541 tttagagatg aaatcatgaa tgcaaacatt cgctctgcgg gtgcttctgt ttctaccgac
      601 tctacgagac ccttctacgg cgatttcaac tacgacgacg gagtgagaca tcgctcgtac
      661 ggtcacggcg gaggattgtc ggcaatttat aatgttggta aactgaaact atcgatagat
      721 ctggaatcct atcggctcca tggcccgtgg ggattgaaat ataagaaaac ggaaagaagc
      781 aatgggagtc ccggatttgc tcctctgtac gtggatacgg aaggaaccat ccgcaaaacc
      841 accagcgtgc tgtctcccgc ggcaggctat gaagtattcg cgggtttttc aattcgtctt
      901 gctgccaggt ttgagcaggc ctcttatcag attcgaagag ccacgatgcc tcttttcctg
      961 ggcggagcca gtccgccaga tttcgcaagc gcgttgtttc tacagaacgt agtctacaat
     1021 ggatttggct tttcagagaa actaaatcgg catgatagcg tacaatatgt ttctcttgga
     1081 atagaaaaga aaatcgattt ctagattcct gccagttcgt cgttccagct acttgagtcg
     1141 cgaggaaggc ttcgcaaaga atcaatcatg gcctcgcgaa atcttcctgg tgccctgcga
     1201 atcgtacgaa tgccaccaat gcaaaacagg gccgcaatga ttgcgggcac catccaggac
     1261 ggtcttccga aaaagtagcg cagaatcaga gtgagagtca acgaccccag cgccggataa
     1321 aacagcaatt gccagaatgc agcccgcaga tggctgaggg aagccgtgat caatatgaat
     1381 ttgcccggag tgctgagtgt gatttctcca tcacagttgg cccatgggtt tctgcctgcg
     1441 ccgtacattt taccccggaa cgttatgcga tcatctatct tctcgcatga ttctactttg
     1501 cggtccatat gcgccatcag tgctgacttt gcaggaagct tcagctctcc cggaatcgac
     1561 gtggggagaa cgaccctgtt ttctagaaat ctcacagttc accgcgtggg tgtttgcccg
     1621 ggcagacaat cgtttttcgt atttttgctt ttctaatttc gggcggtcag ttccaatgcg
     1681 agcatgcgcc atgttgcttt ttgttcactt ttgattttcc tctcatgttc gggcgcgccc
     1741 gttcaggagg gcgccattcg cggtagcgct ggcggttata cgatcgtatt tcctcaaaag
     1801 tggaagattg ttagaccaga cgatgtgacc cttgaggcag tgtcagaaga tgaattggcc
     1861 ggagttcgca tcgtagagca gggcaggatt ggaaagtcag cggaagacat tctgggaaaa
     1921 atcgaaaaac aactcgaagt gcagaattcg cttcctgaaa gcagtcgcac tctggatgca
     1981 aatcttctca aagagagcaa tgctcagcat tgtgccattg gtcagtatgc actgagaaag
     2041 aacggagtgc tccttcgccg caagatctgg gtttttgtga agggtcaaag ggcttttgtg
     2101 atcaccacga atgttccaga aagttacgcc ggagaatatc ctggtgtctt tgaaggcatc
     2161 attaagtctc ttcgcgctca gtagttcatc aggtgatgac tgtaggcctc tctcttccgg
     2221 ttctctctct gaggccggag atctgagatg caatcgtgat gaaatcgctc aatgcctcct
     2281 gggcatccag attgtgtctg gccggatcgg gttcataagc ttctagatag agacgcagag
     2341 tggccccttc tgttccggtt ccactgagac gaaagatgat ccgcgatccg tctacaaaga
     2401 tgattctgat tccttgacct gaacttacgc ttgcatccac tggatcgttg tagctgaaat
     2461 cgtcacagaa tctgatttca tgttttccaa atttctttcc tgtaagttct gcgaagcttt
     2521 ctgctacatg tttcatcagg ccagaggccg cctcggaagg aatgccctcg tagtcatgac
     2581 gagaatagaa attgcgcccg aatttgttcc agtgttcccg catcacagtt tctacagact
     2641 ggtgctttac tgccagaatg ttgagccaga atagaaccgc ccacagtccg tctttctctc
     2701 tcacgtggtt tgatcctgtt ccaaagctct cttcgccgca taacgttact tttcccgcgt
     2761 ccatgaggtt accaaagaat ttccatccgg taggagtttc aaaacaggcc agaccaagag
     2821 actgcgccac tcgatccgcg gccgcactcg ttggcatgga ccgggcaatt cctgccagtc
     2881 ccttcttata tgcaggaacc agtgttgcat tcgccgcgag caccgccagg ctatcggaag
     2941 gatttacaaa gaatcctttt ccgagaatca tgtttctatc tccatcaccg tccgaagccg
     3001 cggcaaaatc tggcccgtct tttccgtagg caatttcaac caggtcatgg gcgtaggtga
     3061 ggttaggatc cggatgaccc cctccaaagt cttcgagtgg aacggcattc atcaccgttc
     3121 cagccgaggc tcccagtcgc ttttgtagaa tttcgaccgc gtagggtcct gtgaccgcat
     3181 gcatggcatc aaacttcatt ttgaagccgc tgaaaa
//
LOCUS       DAOQJL010000459         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19725_length_3211_cov_4.064322, whole genome shotgun sequence.
ACCESSION   DAOQJL010000459 DAOQJL010000000
VERSION     DAOQJL010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19725_length_3211_cov_4.064322"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLG78_16195"
     CDS             complement(<1..685)
                     /locus_tag="PLG78_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24715.1"
                     /translation="MLAQSEKEFTSLGQRATFNRTLRHLVSVTTIVDRVEKGENLADI
                     LNELLDSEMLARDQIGPVLSLVLVSKMDLPFRSRNLPESVNKFEGILAQMKKWPGVDL
                     VIAYHHPNLGIIPINPANEAHWNNAIELKRDELVVVYARAASKKTETAQKALDAFFTL
                     LKGGTPAEDPDFATKPVAAKPVAQRAAAAPAAAAQARPMPAPQARPMPGAPGAMPQPR
                     PMPAAPGAAQ"
     gene            complement(790..2817)
                     /gene="cfpA"
                     /locus_tag="PLG78_16200"
     CDS             complement(790..2817)
                     /gene="cfpA"
                     /locus_tag="PLG78_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HNE24716.1"
                     /translation="MGQFPKSPNKVDPVIPSAVSSRTSIVWEGRDKIVESKKIIDDTA
                     NRVQHFVQTKLPPEVLKDVDIMASIEDKIYNYVNQSYVNMFNRYTVTVEDEMVKKVRE
                     FIDKEELRTLQRYTPREIVEILDKVGGADKFNTGELEKSVVNMYGHLQGHIQRGMNDL
                     ENETNSILRQKTDVGAFVRGENAYSIVKCSFKDSQFKPKTVTDIKLSINILDSELISP
                     IFHYQTTVEHLLKDSISKHITELIDREIDKLKEELVDTGRAELSDGEIMFEKIKLVDK
                     YTDDNVSDPNSRRYTILAKKFLDKIEGLRAEIDQDDYDSMNMREQIKKIIDSENIRNR
                     GFNTAVNTLTSILDTSKLGYQVCDNKKNARECWVREYEDQDDARLPDERYAIRLVYLD
                     QNQIRDLKRAYDARIAAFLREGEAVWDVVHEVYDRTKKRFFGLSRVVKDFEDLARSIM
                     PKAWVKERTTVNNDPDEIHWDKLRFRDAPTTFVEKNNRTYSMEVKNVGEKIRYLRDRL
                     QTMYGYQNPVERVVIDERLNLLEQTFNKYIYEINPHHLQPGLILDVDITTTKRKMYMM
                     KSMANVLNEFLSGVSQGFADAAFASFKRRRSTVRADIEMTFSQEEHKDSASSLAYKGQ
                     EPAAGSMYAAPSAPEPQRAASAGGESGVRGSADVTPKSYEDKLKEMGLKEL"
BASE COUNT          709 a          772 c          819 g          911 t
ORIGIN      
        1 gctgcgcggc ccccggtgct gcgggcattg gacgaggctg aggcatagca cctggagcgc
       61 ctggcattgg gcgcgcctgt ggcgcgggca ttgggcgcgc ctgggctgct gccgcaggag
      121 ccgccgccgc tctttgtgca acaggcttgg ccgctacagg tttggttgcg aaatcaggat
      181 cttctgcggg ggtgccgccc tttagaagag tgaaaaacgc atccagagcc ttttgtgcgg
      241 tttctgtttt cttactggca gcgcgcgcat atacaacaac gagctcgtca cgcttgagtt
      301 cgatcgcatt gttccagtgc gcttcattgg caggattgat tggaatgatg cccaggttcg
      361 gatgatgata tgcaatcacc agatccacgc ctggccactt cttcatctgc gcgagaatgc
      421 cttcgaattt gtttacgctt tctggaagat tgcgagagcg aaatggaagg tccattttgc
      481 tgaccagaac gagagaaaga acgggaccga tttgatcgcg cgccagcatc tctgaatcga
      541 gaagctcatt caaaatgtct gcgaggtttt ctcccttctc cacgcggtcc acgattgtgg
      601 ttacactgac tagatggcga agagtgcgat tgaacgtagc gcgttgtcca agcgatgtga
      661 attctttttc tgattgagcg agcattgtgg cctccggatg accctactaa caaagactgt
      721 ccatttctgc atataaaaaa cccgcaggct atcctgcggg ttttcaggtt tcagagtctt
      781 tttggaatct tacagttcct tgagacccat ctccttgagt ttgtcctcat aggactttgg
      841 tgttacgtct gcgcttccac ggactccgga ttcaccaccg gcagatgctg cccgttgtgg
      901 ttctggagcg gatggtgctg catacatgct acctgccgct ggctcctggc ctttgtatgc
      961 cagggaagat gcggagtcct tgtgctcttc ttgcgagaag gtcatttcga tgtccgcacg
     1021 gacagtcgaa cgacgacgct tgaaggaagc gaacgctgca tccgcgaagc cctgagatac
     1081 gcctgacagg aattcgttca gaacgtttgc catagacttc atcatgtaca tcttgcgctt
     1141 cgtcgtagtg atgtctacgt ctagaatcag gcccggctgg aggtggtgcg ggttgatttc
     1201 atagatgtac ttgttgaatg tttgttcgag aaggttgaga cgttcgtcga tcacaacacg
     1261 ctcaaccggg ttctgataac catacatggt ttgcagacgg tcacgcaggt agcggatctt
     1321 ttctccaacg ttcttcactt ccatagagta agtacggttg ttcttttcta cgaaggtagt
     1381 tggcgcatcg cggaaacgga gtttatccca gtggatttcg tccggatcgt tgttcacagt
     1441 ggtgcgttct ttcacccatg cttttggcat gatagaacgg gccaggtctt cgaagtcttt
     1501 cactacacgt gacagtccaa agaaacgctt cttggtgcgg tcatacactt catgaaccac
     1561 atcccacact gcttcgcctt cgcgaaggaa tgcagcgatg cgtgcatcgt atgcgcgctt
     1621 gaggtcgcgg atctggttct ggtcgaggta tacgagacgg attgcgtaac gttcgtctgg
     1681 aagacgcgca tcgtcctgat cttcgtattc acgcacccag cactcacgag cgttcttctt
     1741 gttatcgcag acctggtaac ccagtttgga agtatcaaga atagaagtca gagtgttgac
     1801 tgcagtgttg aaaccgcggt tacgaatgtt ctcggagtca atgatcttct tgatctgctc
     1861 gcgcatgttc atggagtcat agtcatcctg atcgatttca gcgcgaaggc cttcgatttt
     1921 atcgaggaac ttcttggcca gaatcgtgta acgacgagaa ttcggatccg atacgttgtc
     1981 atcagtatac ttgtctacga gcttgatctt ttcgaacatg atctcgccgt ctgacagttc
     2041 tgcacgacct gtatccacga gctcttcttt gagcttgtcg atctcgcgat cgatcagttc
     2101 agtgatgtgc ttggagattg agtctttcag cagatgttca acagtagtct ggtagtggaa
     2161 gatcgggctg atcaattctg aatccagaat gttgatcgag agcttgatgt ccgttacagt
     2221 ctttggcttg aactggctgt ctttgaaaga acattttacg atggagtaag cgttctcacc
     2281 acgaacaaac gcgcccacgt ctgttttctg acgcagaatc gagttggttt cgttttcgag
     2341 gtcgttcata ccacgctgga tgtgcccctg aaggtgccca tacatgttta cgactgactt
     2401 ttcgagttca ccagtgttga acttgtccgc gccacctact ttgtcgagga tctcaacgat
     2461 ctcgcgagga gtgtaacgct gcagagtacg gagttcttct ttgtcgatga actcgcgaac
     2521 ttttttgacc atctcatctt caactgttac agtgtaacga ttgaacatgt tcacataaga
     2581 ctggtttaca tagttgtaga ttttgtcttc gatgctcgcc atgatgtcga catctttgag
     2641 tacttctggc ggcagttttg tctgaacaaa gtgctgcaca cggttggccg tatcatcgat
     2701 gatctttttg gattcgacga ttttgtcccg accttcccaa acgatagaag tacgcgacga
     2761 caccgcgctt ggtattacgg gatctacttt attgggactt ttgggaaatt gacccattcc
     2821 tttcctccgg ggttatgagc aggagacaca atatctcttt tcagattatg aagataggtg
     2881 attccgcagg caaccatcct ttcaacaaaa aaatcatgcg cgattcggaa ttttgaacgt
     2941 tctgtgaagt gtgacacaaa tgctacataa tccgtgctta tttgcatcaa tccagaggca
     3001 tttctcaaaa aaaaacacca gactgcaaaa aaaatacgct ctccactcaa tcgaggcatt
     3061 gcgtcggtcg agcgcgagat caaaaatctt tgtcagtgaa gatgtcttct ctcgtgcgtt
     3121 tcacttcctg tctgtctgaa tcactgcttc ttctggagtc agcggaagtc tttggctcag
     3181 tgcctggcaa aaataattct acaattgtat c
//
LOCUS       DAOQJL010000460         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19727_length_3211_cov_3.596008, whole genome shotgun sequence.
ACCESSION   DAOQJL010000460 DAOQJL010000000
VERSION     DAOQJL010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19727_length_3211_cov_3.596008"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..684)
                     /locus_tag="PLG78_16205"
     CDS             complement(139..684)
                     /locus_tag="PLG78_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNE24717.1"
                     /translation="MFRVQKGTLKGKSISMPPESRGHSCFTPGLVKEALFQLVENRLE
                     TEPSDYAFFDLCAGSGQIAIEALSRGYHPVHAAELDQGRFEFLIRQVRAQKYDIRLHR
                     KDFRKMSPFIEGRSVVFLDLPYTFWTPLPALITDFLGSLREPAPGGHRIIVIQGPAAW
                     PDWEPHPYGGTVLSLSEQKMV"
     gene            complement(684..911)
                     /locus_tag="PLG78_16210"
     CDS             complement(684..911)
                     /locus_tag="PLG78_16210"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNE24718.1"
                     /translation="MLPLRLTFMLIGALILALLAFGNPEPVPIRFLFWTDEIALYKLL
                     LSAVLFGIILAVLYIGHVRYLRRHLGDRFRR"
     gene            complement(913..1110)
                     /locus_tag="PLG78_16215"
     CDS             complement(913..1110)
                     /locus_tag="PLG78_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24719.1"
                     /translation="MWDRDDEDLEEDLDWDEDVEDDEDSEHASFVCEDCDYRWDESQE
                     EDMDTGMHVCPMCGSVNVTQL"
     gene            complement(1157..1912)
                     /locus_tag="PLG78_16220"
     CDS             complement(1157..1912)
                     /locus_tag="PLG78_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNE24720.1"
                     /translation="MLSRDEITRYSRNILLPEVRKSGQEKLKSSSVLVVGAGGIGSPV
                     LTYLAASGIGRIRCVDSDHLDLTNLQRQVLYRTGGVGSPKIDLAREQIHSLNPNLELD
                     FRMERFSDKNAKDLVTGMDLVIETSDNFPTKFLVSDVCVENRIDYIAGGILRFDGQVL
                     CVRPGQSACYRCIFENMPEEGSVPNCAAAGVIGAVAGVIGSIMAGEAVKILLGRDESF
                     GLLQQYNLLESRFRSLRITRRSECTCGRKSLPK"
     gene            complement(1914..2381)
                     /locus_tag="PLG78_16225"
     CDS             complement(1914..2381)
                     /locus_tag="PLG78_16225"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE24721.1"
                     /translation="MKNVPGLLILVLLASFCKTAVPAGEKTSDQYIADLSSGDMSLQI
                     TAARKLGERKEEQAIPALVSLLDSNPAPEVAWNVAASLGAIGKPGPATAALQRSANSP
                     SPSVKYASIAALTNLHDKDQKAQNVQAASQMRDSTRDDLLKDLADKAATLMAR"
     gene            2436..2864
                     /locus_tag="PLG78_16230"
     CDS             2436..2864
                     /locus_tag="PLG78_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24722.1"
                     /translation="MPSLLHPFIVHVPIAFALFYVPFAWLCIRDRIQWSWFTLYSLCL
                     CASLYGAMASGSVDRAIVEKNRPSISSPLEEHEGSAQKAFFFALAALALSFAGKTRFR
                     EAAKIACGLIALAVLASTIQTARLGGNLVYKHGAAEAHAR"
     gene            2861..>3211
                     /gene="eutB"
                     /locus_tag="PLG78_16235"
     CDS             2861..>3211
                     /gene="eutB"
                     /locus_tag="PLG78_16235"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNE24723.1"
                     /translation="MIWAVTGATGFLGRHLVEASLSRGATVRALSRTIVPPSVEHFEG
                     DLLEPDPAGIAASIVDGLLYGSGDAVIGINPATDSPAAVRTLLELIDAVRQRYDAPVQ
                     SCVLAHITTQLQALE"
BASE COUNT          742 a          878 c          825 g          766 t
ORIGIN      
        1 atcgagtaaa gtgcaatgat tataagtgct atgatatcca tgggaaaaga cgggttcctc
       61 gcgcaaattt gcgctagata tgttttcgga gcatgttttt tgagacgcgt ttgcggtcaa
      121 attttttacg attccgcgtc agaccatttt ttgctcggat aaggacagga ctgtgccgcc
      181 gtacgggtgt ggttcccagt cgggccatgc tgcggggccc tggatcacga tgattctatg
      241 gccgccgggg gcaggttcac gaagagatcc aaggaaatcg gtgatcaaag caggcaatgg
      301 ggtccagaac gtatacggga ggtccagaaa gaccacggaa cggccttcga taaaagggct
      361 catcttgcgg aaatcttttc tgtggaggcg aatgtcgtat ttctgagcgc gaacctgccg
      421 gatcaggaac tcgaatctac cctgatccag ttccgctgcg tggactggat gatacccgcg
      481 gctgagagcc tcaatggcga tctggcctga gcccgcgcac aaatcaaaga aagcatagtc
      541 cgaaggctca gtctccagtc tgttttccac gagctgaaaa agtgcctctt tgaccagtcc
      601 aggagtgaag caactatggc ccctggattc gggcggcatg gaaatgcttt tgcccttgag
      661 agtccctttc tggacgcgaa acatcagcga cgaaagcgat caccgaggtg acgccgcaaa
      721 tatcgcacat ggccaatgta taaaactgcc agtatgattc caaaaagcac agcggataaa
      781 agtagcttgt agagcgcgat ttcgtccgtc cagaacagaa atcgaatcgg aaccggctcc
      841 ggattcccga aggccaacag ggccagaatc aaagccccga tcaacatgaa tgtaagccgg
      901 agtggaagca cgctatagct gggtcacatt cacgctgccg cacatcgggc aaacatgcat
      961 tcctgtgtcc atatcctctt cctgagattc gtcccagcga tagtcgcagt cttcgcaaac
     1021 gaagcttgcg tgctcgctgt cttcatcatc ttctacgtct tcgtcccagt caaggtcttc
     1081 ctcaagatct tcgtcgtctc tgtcccacat aggctatggg ctatcccata cgaccgtcag
     1141 aaaatgccag acttttttat ttcgggaggg attttctgcc acaggtgcat tctgaccttc
     1201 ttgtgattcg caggctgcga aagcgacttt ccagcaaatt gtactgctga agaaggccga
     1261 atgactcatc ccggccaagc aaaatcttca ccgcctcccc ggccatgatg ctccctatga
     1321 caccggccac cgctccaatc acaccggccg ccgcgcaatt cggcacgctc ccttcttctg
     1381 gcatgttctc aaaaatgcat cgatagcagg cactctggcc gggccgcaca cagagaacct
     1441 gtccatcaaa tcgaaggata ccacctgcaa tatagtcgat ccgattttct acgcacacgt
     1501 cgctgacgag gaacttggtg ggaaaattat cagaggtctc aatcacaaga tccatcccgg
     1561 tgaccaggtc ctttgcattc ttgtctgaga atcgttccat tctaaaatcc aattccagat
     1621 ttgggttcaa tgaatggatc tgttctcgcg ccagatcaat cttcgggctt cctacaccgc
     1681 ccgtgcgata cagtacctgt ctttgtaaat ttgtaagatc gagatgatca gagtccacgc
     1741 agcgaatcct gccaattcca gacgcagcca gatacgtgag tacgggagac ccaattccac
     1801 cggcaccgac tacgagcaca ctcgatgatt tgagtttctc ctgacccgat ttacgcacct
     1861 cgggcaggag tatatttcta gaatagcggg tgatttcgtc ccgcgaaagc aacttacctg
     1921 gccatcagag tcgcggcttt gtctgcaaga tctttgagaa gatcatcacg cgtggagtct
     1981 cgcatctgag aagcagcctg cacattttgc gctttctgat ccttgtcatg caaattggtc
     2041 agcgcagcaa tgctcgcata tttgacggaa ggggaaggag agtttgcaga tctctgcaga
     2101 gctgcggtgg ccggacctgg tttgccaatc gcacccagag aggcagctac attccatgcc
     2161 acttccggag cgggatttga gtccagcaat gacactagag caggaattgc ttgctcctct
     2221 ttgcgttcac cgagctttcg cgctgcggtg atttgaagag acatatctcc ggagcttaga
     2281 tccgcgatgt attgatctga agttttttca cctgccggca ctgctgtctt gcaaaaggat
     2341 gcaagcaata ctagaatcaa tagacccgga acattcttca tcttgccttg taaaatatct
     2401 ggcattccag gcatgtcaac ggaaaaaacg atccaatgcc ttctctattg catcccttca
     2461 tcgttcatgt gcccattgcc ttcgcgctgt tctatgttcc tttcgcctgg ctttgcattc
     2521 gtgaccgcat ccagtggagc tggtttacac tctactcgtt gtgcctttgc gcttccctct
     2581 atggcgccat ggccagcggg tctgtcgatc gcgccatcgt agagaagaat cgcccttcca
     2641 tcagctctcc gctcgaagaa cacgaaggct cagcgcaaaa agcgttcttt tttgctctgg
     2701 cagcgctcgc actgtcgttt gctggcaaaa ccagatttag ggaggccgcg aagatcgcat
     2761 gcggactcat cgcccttgcc gttctcgcaa gcaccatcca gactgcgaga ctgggcggca
     2821 acctggtata caagcacgga gcagcagagg cacacgcgcg atgatctggg ctgttaccgg
     2881 agcaaccggg tttctaggca gacatcttgt ggaagcttct ctttcgaggg gagcaaccgt
     2941 gcgcgcactc tcgcggacca ttgttccacc atccgtagaa cactttgaag gagatcttct
     3001 cgaacctgat ccggcgggga ttgccgcgag tatcgtggat ggactgctgt atggcagtgg
     3061 cgacgcggtc attggcatca accctgccac cgatagcccg gctgcggtgc gtacgctgct
     3121 ggagttgatt gatgcggtgc ggcagcgtta cgacgccccg gtgcagagct gcgttctggc
     3181 gcatatcacc acgcaactgc aggctctgga g
//
LOCUS       DAOQJL010000461         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19750_length_3208_cov_4.043768, whole genome shotgun sequence.
ACCESSION   DAOQJL010000461 DAOQJL010000000
VERSION     DAOQJL010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19750_length_3208_cov_4.043768"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1045)
                     /gene="recA"
                     /locus_tag="PLG78_16240"
                     /pseudo
     CDS             complement(44..1045)
                     /gene="recA"
                     /locus_tag="PLG78_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004766281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            complement(1179..1790)
                     /locus_tag="PLG78_16245"
     CDS             complement(1179..1790)
                     /locus_tag="PLG78_16245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE24724.1"
                     /translation="MKFSEVFEEKIWNASNILTMTRMILLPVYVWLGGAYREDPGGPM
                     LWVLLSLISGAVISDFLDGFLARKLHQITKLGQYLDPISDKTVTLVAMLDCALHYGFP
                     WFVFGFCVFREILGVWVGSYLYFKRDMQGSPNMWGKFGVGAVALSVIWHVLTPYFKLK
                     GLTGIWLDAWISAALIFFIFLAGMIAYGRTYWRIILHGKTKST"
     gene            complement(1787..2185)
                     /locus_tag="PLG78_16250"
     CDS             complement(1787..2185)
                     /locus_tag="PLG78_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24725.1"
                     /translation="MVSTRKLVLAFSLLAMACGGKPQVDRGLSVSPEKLSGRFAMVPS
                     ATQEEIRFKEDGLVEMKSEESSAKGLYALDASSLRFRFENGDYGIFLRSAFHPREWRG
                     FYRGEVRILKRLEGAKNLDRSESEGTTSKP"
     gene            complement(2164..>3208)
                     /locus_tag="PLG78_16255"
     CDS             complement(2164..>3208)
                     /locus_tag="PLG78_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24726.1"
                     /translation="ALQEVREQIEEITASLENAAYIPAHDVNFQIDAASISLSSDLKF
                     DAKGKALDHAFTLHASGPFRFGDSPDLFSGGPLVIEGEVATLDLPRIARAMFGAHSHL
                     KGIGTAEDAEKAEDTGPLWTQRRKTQAQANLLSYFSMNGSIVAKGIAGVTNESVPINI
                     SKNGGGISARAGYQKDGFRLEGDYALGFDSDLPHQTIHLKYDNEQNNVSMEYLTGDEQ
                     SPRSISLDYNSSMDGLLPADLFFKSTSTMYFRARGVDTRKLDPAPLVFSIAGIKDSKI
                     DELEFQRTTEGGRTMWTQVHASRGEANWTGAGDFSSQEGGKATFFVNAPGGYSRRIDL
                     GVTEAGKWYPREN"
BASE COUNT          753 a          852 c          762 g          841 t
ORIGIN      
        1 ctattgttcc tgaagttatt caagaatgaa gaagaatttc ctgttccacc tgatcggcgg
       61 tttttggatt ttcgcgcaga tactggcaag tattctcgcg accctggcct actcggtccc
      121 ctttgtagga ataccaggtt ccggttcttt ccagtatacc aagtgaagtg cccagatcca
      181 gaaggcagcc ttctcggctg attcctgaat caaacagaat atcaaattct gcctggcgga
      241 agggaggagc cattttgttt ttcactactt tgactctcac acgatttccg tatgcctcat
      301 ctcccttctt gagagtttca gtgcggcgaa tgtccagacg gatagaagca tagaacttca
      361 atgcgtttcc acctgtggtt gtttcaggag aaccgaacat cacaccgatc ttgttgcgaa
      421 tctgattcac gaagatcaca gtagtgttgg atcttgaaat agtgcctgtg agctttcgca
      481 tggcctggct catgagtctt gcatgaagac ccatttgcgc atctcccatg tttccttcca
      541 gttctgcttt tggaacgagt gctgccactg aatccacgac cacaatatcc acggcattgg
      601 accgcactag agactcagca atttcaagag cctcttctcc attgtccggt tgcgcgacca
      661 gcaggtcatc aatgttgact cctagctttc gcgcgaactg cggatccagt gcatgctctg
      721 catccacgta ggctgcaatg ccccccgcct tctgtgcatg cgcgactgtg gaaagacaaa
      781 cagttgtctt gccggatgat tcaggtccaa agatttctac aatgcgaccg cgcggaagtc
      841 cgccgactcc aagagccata tccagagtga gcgctcctgt ggggatggtt cccacttctt
      901 gaaaagcact ctcatctccc agtttcatga tggagccttt cccaaattgc ttttcaattt
      961 gctggatggc tcccaccacc gcctttttgc gttcctctaa ctgaggattg ggagcattgc
     1021 ggtccggtct tctggtctct accacttcca gtttccttgc cttcgtagcc atttacatct
     1081 cctcgggctg ctcttccagg aagttgcagc caatcttttt tgccatccta tttgaccggt
     1141 gtgacataaa aagccatacc agaactaggt aacatttgtc aagtagattt agttttaccg
     1201 tgaaggataa ttcgccagta ggttcgccca taggcgatca ttcctgccag gaatatgaag
     1261 aaaataagag cagcagaaat ccatgcgtcg agccagattc cagtaagacc cttcaatttg
     1321 aagtacggag tcagaacgtg ccagatcaca gaaagggcca cggctcccac tccaaattta
     1381 ccccacatat tggggcttcc ctgcatatct ctcttgaaat agaggtagga tccgacccag
     1441 acaccgagta tttcccgaaa tacgcagaac ccaaaaacaa accacgggaa tccatagtgg
     1501 agggcgcagt cgagcatggc caccagagtc accgtcttgt ctgagatggg atccagatac
     1561 tggcctagtt ttgtaatctg atgcagtttg cgcgccaaaa atccatctaa gaaatcagag
     1621 atcacagcgc cggaaatcaa agagaggagg acccacagca tggggccgcc cgggtcttcc
     1681 cggtaggctc cacccagcca cacgtagacg ggcagcagga tcattctggt catagtcaga
     1741 atgttcgaag cattccagat cttctcttca aacacctctg agaatttcat ggcttgctag
     1801 tggtgccctc cgattcagat cggtcaaggt ttttagcccc ttccagcctt ttcagaatgc
     1861 gcacttcacc gcggtaaaag cctcgccatt ctctgggatg aaatgcgctg cggagaaata
     1921 ttccatagtc tccattttca aatcgaaacc tgagagaaga tgcgtcgaga gcatacagac
     1981 ctttcgcgga tgattcttcc gatttcattt ctacgagccc gtcttctttg aaacgaatct
     2041 cttcttgcgt ggcgctggga accatggcaa atcgaccgct cagtttttct ggagagacag
     2101 acaggccgcg atctacctgg ggttttcccc cgcatgccat cgcgagaagt gagaaggcga
     2161 gaactagttt tcgcgtggat accattttcc tgcctcagtc actccaagat caatccgcct
     2221 ggaatagcca ccaggcgcat tcacaaaaaa agtagctttg ccgccttcct ggctggagaa
     2281 gtcgcctgcg cccgtccagt ttgcctctcc cctggatgcg tgaacctggg tccacatggt
     2341 tctgccacct tctgtagttc tctggaattc tagctcgtct atcttgctat ccttgatacc
     2401 tgcgatggaa aaaacaagag gcgctggatc gagcttgcga gtgtccacac cgcgcgcgcg
     2461 gaaatacatt gtgcttgtag atttgaaaaa aagatccgcg ggcagaagtc catccatgga
     2521 tgagttgtaa tccagcgata tagacctggg gctctgctcg tctccggtta gatattccat
     2581 ggatacattg ttttgctcgt tgtcgtactt cagatggatg gtctggtgcg ggaggtctga
     2641 atcaaacccg agagcgtaat cgccttccag gcgaaatcca tccttctggt atccggcgcg
     2701 agcactgatt cctccgccat ttttgctgat gttgatgggg acggattcgt ttgtaacgcc
     2761 cgcgattcct ttcgcaacga tggatccatt catgctgaaa tagctgagca agtttgcctg
     2821 agcctgagtc tttcttctct gagtccagag gggacctgtg tcttccgctt tttccgcatc
     2881 ttctgccgtg ccgattcctt tgaggtggga gtgtgctcca aacatcgcgc gggcaattcg
     2941 cggtaaatcc agagttgcta cttccccttc aatcacaagc ggaccaccgg aaaataagtc
     3001 aggactgtct ccaaaacgaa agggacccga agcgtgaagt gtgaacgcat gatcgagtgc
     3061 ttttcctttc gcatcaaatt tcaaatccga tgaaagagaa atgctcgcag catcgatttg
     3121 aaaattgaca tcatgtgcag gaatgtatgc ggcattctcg aggctcgccg tgatctcttc
     3181 gatttgttct cgcacttcct ggagagca
//
LOCUS       DAOQJL010000462         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19797_length_3202_cov_4.945980, whole genome shotgun sequence.
ACCESSION   DAOQJL010000462 DAOQJL010000000
VERSION     DAOQJL010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19797_length_3202_cov_4.945980"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLG78_16260"
     CDS             <1..452
                     /locus_tag="PLG78_16260"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNE24727.1"
                     /translation="TWCVLKKMFPNADIPVVQLSLDAAKSGEQHMAIGRELRELRNEG
                     VLILASGNIVHNLGAADFSGRRGTYEWATRFDNTVTSLLDQREFSKLCAIESLGNDAA
                     LSAPTPEHYWPLLYALGAAEEQDRLTYPVTGMDLASISMRSVMFSES"
     gene            complement(465..2159)
                     /locus_tag="PLG78_16265"
     CDS             complement(465..2159)
                     /locus_tag="PLG78_16265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE24728.1"
                     /translation="MIHKREFHDLEKKFDLVVIGGGITGISVAREAAVRGIRVLCVEK
                     NDFACATSSATSKLLHGGLRYLESYEFGLVRESLSERHIMGLAAGHLSRPMQFLVPMF
                     EWSNPGRFIMQMGLSTYDLLAYDRNLGTPEDKQIPPAKWISREEFLKLEPKIESKGLK
                     GGFVYYDYQSTYPERLALAFVKTAVQAGAVMLNHCQATGFGFKAGTKQIESVQIQDTL
                     SGKEYTAKGSVFVNASGPWMDQVLGLAERSPLHKVQKSKGIHLLTDRIIGEQTLFLRN
                     KKGQHCLLLPWEGMTLIGPTDSPYEGSPDDLSPVEEDITMLMDLVNELLPGRPLTREK
                     IRHVPIGIRPLVFSGKSTYRASRKHEIYDHAADQDKIEGLVSVLGGKWTTSRKLGEDV
                     LHACLPKLRSFSPGIQIKDVRTDREPLHGYPTFGASSTEIWEQIALMNHRVPDNVLNH
                     LYTLYGTDYQEVLEIARKEPSLLKRISDEPGRYDIMAQIVYAVENESAHTLSDILSRR
                     LALGSFGYPGDTAAKRAADLAGKLLKWSSSRKTEELKAYRLSYPLERLSTDSKKKK"
     gene            complement(2156..>3202)
                     /locus_tag="PLG78_16270"
     CDS             complement(2156..>3202)
                     /locus_tag="PLG78_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE24729.1"
                     /translation="PRAFTIDRTFAQEFATVFHESSPLFLSAPYAQAHGFADMLVHPL
                     QVFNVILSLGVQNNSEKAIANLGYYNVQFLKPVYPGDTLTGRTRVLSKKVRGEGKPGI
                     VHVNTLGLNQRGEVVIQYERKIMIPPGDGKSAPKRGDANLPFPVVKDPSCELPDAKSL
                     STDMKHHTLSNTYFEDFEAGQILVHKNMRTITDEHVPWTYRMGNTHPLHYDRLYSAGL
                     SGPMSGEPIVYGGLVFGWLCGLASRDLTENMLWDLGYTEGYHTAPAISGDTVGSISRI
                     VAKEEGPMPNTGILHVQLIGVKNVKTKEAFDKHGADLFIKENDKKDMGKEKIPEKIFE
                     IERRILLKKKGYGA"
BASE COUNT          765 a          785 c          803 g          849 t
ORIGIN      
        1 gtacctggtg tgttcttaag aaaatgtttc cgaacgctga catccctgtg gtgcagctga
       61 gcctggacgc ggccaaaagc ggagaacagc atatggcgat cggtcgggaa ttgcgcgaat
      121 tgcgcaacga aggagtgctg attctcgcaa gtggcaacat tgttcacaat ctgggtgcag
      181 ctgattttag cggacgcaga ggaacctatg aatgggccac gcgttttgac aatacagtca
      241 cttccctgct ggaccaacga gaattttcga agttatgcgc cattgaatcg cttgggaacg
      301 acgccgctct atctgcaccc acaccggaac actactggcc tcttctgtat gctctgggag
      361 ccgcggagga acaggatcgc ctaacctatc cagttacagg gatggatctt gcatccattt
      421 ccatgcgatc cgtgatgttt tccgaatctt gactcgatcg gggattattt ctttttcttc
      481 gaatcagtag aaagtctttc gagaggataa gacaatctgt atgccttgag ttcttctgtc
      541 ttcctggaag aactccactt cagaagtttg cctgcaaggt ctgccgctcg ctttgccgca
      601 gtatctccgg ggtagccaaa gcttccaagc gccagtctcc tggaaagaat gtcagacaga
      661 gtatgggcgg attcattttc tacagcgtag acgatctgag ccatgatgtc atatcttccg
      721 ggctcgtcag aaattcgttt cagcagagaa ggttcttttc gcgcaatctc cagcacttcc
      781 tggtagtcgg ttccgtaaag cgtatagaga tgattgagaa cgttatccgg cactctgtga
      841 ttcatgaggg caatctgttc ccatatttcg gtggaagaag caccaaacgt aggatatccg
      901 tgcagtggct cgcggtctgt gcgaacatct ttgatttgaa ttccaggaga aaacgagcgt
      961 aactttggca ggcatgcatg cagcacatct tctccgagtt tcctggaagt agtccatttg
     1021 cctcccagga cggaaacgag gccctcaatc ttatcctggt ctgcagcgtg atcatagatc
     1081 tcatgctttc tggaagctct gtaggtagac ttacctgaaa aaacaagtgg acgaattccg
     1141 atcggcacgt gccgtatctt ttcgcgcgta agaggcctgc ccggcaagag ctcattgacc
     1201 agatccatca gcattgtgat gtcctcttcc actggactga gatcgtcggg acttccttcg
     1261 taaggcgaat ccgtgggacc tatgagagtc atgccctccc atggaagcag aagacaatgc
     1321 tggcctttct tgtttcgcaa aaacagagtc tgctccccga tgatgcgatc cgtaagtaaa
     1381 tgtatacctt ttgatttttg aactttgtgg agaggagaac gttccgcaag accaagaacc
     1441 tgatccatcc agggcccgga cgcgtttaca aatacagaac ctttcgcagt gtattctttc
     1501 ccgctcagag tgtcctgaat ctgaacagat tcgatctgtt tggtgcctgc tttgaagcca
     1561 aagccagtgg cctgacaatg attgagcatc accgcgcccg cctgcaccgc cgtttttaca
     1621 aaagcaagcg ccagcctttc aggatatgta ctctggtaat cgtaatatac gaatccgcct
     1681 ttcaagcctt tcgactcaat ctttggttcg agcttcaaaa actcttctct ggaaatccat
     1741 tttgcaggtg gaatctgttt gtcttctgga gttcccagat tgcggtcata cgccagaaga
     1801 tcataagtgg aaagacccat ctgcatgatg aatcgacccg gattgctcca ttcaaacatg
     1861 ggaacaagaa attgcatggg ccgactgagg tggcccgcgg ccagtcccat gatgtgacgc
     1921 tcagacagag actcgcgcac taggccaaat tcgtaactct ccagatatct cagaccgccg
     1981 tgaagcagtt tggaagtggc agaggaagtg gcgcaggcga aatcattttt ttcaacgcag
     2041 agcacgcgaa ttccgcgaac ggcagcttcg cgagcaactg aaatgcccgt tatgccgccg
     2101 ccaatgacga caaggtcaaa tttcttttcc aggtcgtgga attcgcgctt atgaatcacg
     2161 cgccgtagcc tttctttttc aacagaatgc gacgctcaat ttcaaagatc ttttctggga
     2221 tcttctcttt gcccatgtct ttcttatcgt tctctttgat gaagagatct gcaccgtgct
     2281 tatcaaacgc ttcttttgtt ttgacgttct ttactccaat caattgaaca tgaagaatgc
     2341 cagtgtttgg catggggcct tcttccttgg caacaatgcg cgagatggaa cctacagtgt
     2401 cccctgaaat agccggagcg gtgtggtaac cttctgtgta gcccagatcc cagagcatat
     2461 tctctgtaag atcgcgactg gccagaccgc ataaccatcc gaagacgagt ccgccatata
     2521 caatcggttc tccgctcatt ggaccggaga gacctgctga atagagacga tcgtagtgaa
     2581 gtggatgtgt attgcccatg cgataggtcc atggcacatg ctcatccgtg atggttcgca
     2641 tgttcttgtg caccaggatc tgtcctgctt cgaaatcttc gaagtatgta ttggacagag
     2701 tgtgatgctt catgtcggtg gaaagagact tagcgtccgg cagttcgcag gatgggtctt
     2761 tgactaccgg gaaaggaaga tttgcatctc cgcgctttgg agcggattta ccgtctcctg
     2821 gtgggatcat gatctttctt tcatattgga tgacaacttc cccgcgctgg tttagaccca
     2881 gggtgttgac atgaacaatt cctggctttc cttctccgcg tactttcttg gaaagcactc
     2941 tggtgcggcc tgtgagtgta tctcctggat aaactggttt caaaaattga acgttgtaat
     3001 accccaggtt tgcgattgcc ttctctgaat tgttttgaac gcccagggag agaatcacat
     3061 tgaacacttg cagaggatga accagcatat ctgcaaatcc gtgcgcctgc gcgtaagggg
     3121 cagacaggaa gagaggagaa gattcgtgaa atactgttgc gaattcctgt gcaaatgtgc
     3181 gatcaatggt aaatgcacgc gg
//
LOCUS       DAOQJL010000463         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19811_length_3201_cov_3.792435, whole genome shotgun sequence.
ACCESSION   DAOQJL010000463 DAOQJL010000000
VERSION     DAOQJL010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19811_length_3201_cov_3.792435"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLG78_16275"
     CDS             complement(<1..181)
                     /locus_tag="PLG78_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24730.1"
                     /translation="MNAKRRSGQPPTRVGGFLSRPVQFLFAIAFVLGACVFAALHFSN
                     GSRRTPTVAVQEKEQG"
     gene            complement(194..1264)
                     /locus_tag="PLG78_16280"
     CDS             complement(194..1264)
                     /locus_tag="PLG78_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24731.1"
                     /translation="MMKRVILLLLPMLALGSELGASTLNQNVSWTIDRAGTTAKYRLV
                     AYGDSIYAGYNGSINNAAKYSAPTVDAEYLSALWNADIESVRRTKSGAVAKDVYDNKI
                     VAEKSYMQATTTRVVTFEMCGNDGLQARTAFKKQTGTCDYTVLSTAEANCKTYVGKAM
                     DYINANTYSGTKVKVISNLYYPGYNADNTQSTCKDATTGATVKMQDKFLPSIARMNYW
                     MCELARQKGFQCADNFAAYMGSDYDKNSDGQVDSAALRYVAGESESSYVTRITTTLRS
                     TIIDANTHFVSAGSTYDFIQSDDTHPTYTGGTVSAGLGGGTTSSGAPRYTSFTGGKNP
                     IWNNYGHELMGWAVSVFNTVAP"
     gene            1789..1971
                     /locus_tag="PLG78_16285"
     CDS             1789..1971
                     /locus_tag="PLG78_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24732.1"
                     /translation="MFRNLLHLADSKSPLARGIAVEALTALRHPDAEKLRKRKPANDL
                     EKARYSSAQNISGFRD"
     gene            2102..3115
                     /locus_tag="PLG78_16290"
     CDS             2102..3115
                     /locus_tag="PLG78_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24733.1"
                     /translation="MNQIQGGLITMSSAITPVAVNSVDMSRSMVLCYFRTTQSDPASV
                     PTCELTSSTQIVVQTGGSSVNYFAQWYLVEFAAGVVAQRGNTTFVPGDFSQSITLPTA
                     VDTAHSFVLLTSRMGSLNQTLDSERTIRGVLSGPTTLTLSRNVAGTANVDVHWQVVQL
                     SDTTVQSGTLTIGAGSSTSTAAISPVNLSKSFIFFNTRADAASGGFEEEYLVRASFAD
                     SSTVSFSRGASNNTVDVSYFAVQMNDASQVQAGSAATSSFAAPNTTTQMTSALNAVNP
                     NTTILAASSSVQNLGGGQQADLDSGSWVPQIVSPTLVQMDRGSSQDRFSQVDYFAVQF
                     VAQ"
BASE COUNT          694 a          817 c          828 g          862 t
ORIGIN      
        1 cgccttgttc tttttcttgc accgcgaccg tgggagtgcg acgtgacccg ttcgagaagt
       61 gaagtgctgc gaagacacac gcgccaagca cgaatgcaat cgcgaagagg aactgaaccg
      121 ggcgagagag gaagccaccg actcgtgtcg gtggctggcc tgaacgacgt ttagcgttca
      181 tgttgtcctt ctgtcagggt gcaactgtgt taaacacaga cacagcccag cccataagtt
      241 catggccgta attgttccag attggattct taccgccagt gaaagaagtg tagcgaggtg
      301 cgcctgaact tgtagttcct ccgcccagac cggctgatac tgttccacct gtgtaggtag
      361 ggtgtgtatc gtcagactgg atgaaatcgt acgtgcttcc tgctgatacg aaatgagtgt
      421 tcgcgtcaat gatcgtggag cgaagagttg tagtgatgcg cgttacatag gaagactcgc
      481 tttcgcctgc aacatagcgc agtgctgctg agtctacctg gccatcgctg tttttgtcgt
      541 agtctgagcc catgtatgcc gcgaagttat ccgcgcactg gaagcccttc tgacgcgcga
      601 gttcgcacat ccagtagttc atacgtgcga tggatggcag aaacttatct tgcatcttga
      661 cggttgcgcc agtagtcgca tctttgcaag tgctctgtgt gttgtcagca ttgtatccag
      721 ggtagtacag gttagagatt actttgactt ttgtgcctga gtacgtgttt gcgttgatgt
      781 aatccatcgc tttgccaaca tacgttttgc aatttgcttc cgcggtgctc agtactgtgt
      841 aatcacatgt gccagtttgt tttttgaaag ccgtgcgagc ctgaagtcca tcgttgccgc
      901 acatttcgaa agtaacaacg cgagttgttg ttgcctgcat gtaggatttt tcagctacga
      961 tcttgttgtc atacacgtct ttggcaacgg cacctgactt ggttctgcga acgctttcaa
     1021 tgtccgcgtt ccagagagca gacagatatt ctgcgtctac tgtaggcgcg gaatactttg
     1081 ccgcgttgtt gatagaacca ttgtagcctg cgtagattga atccccgtat gcaaccaggc
     1141 gatacttcgc ggttgtaccc gctctatcga tggtccacga cacattctgg tttaatgtgc
     1201 tcgcgcccag ctctgacccc agggccagca taggaagaag gagcagaatc actcttttca
     1261 tcatttttat tctcctcagt tcggtttgtt ttactcagag cttcgtattc tactctgtgc
     1321 tgttgaactg accgaccctc tagagcaagt cctgtgccag ggtagtcacg atgattcgcg
     1381 ctggttttcg cgcttgattc ggcagaagca gtgtcgaatt cttagatttg tccattttgc
     1441 ggactgtcca tattcggtcg gtcagcgcaa acaggcatga atcccgtcta acgcataaga
     1501 ggaggcatca tgaaatctga gaagaagatc ctgttcgcgc tcatcgccct gtggctgtct
     1561 tcaaacgcgt tgcatgcaga ccaggagctt caaacctacg gcccgcctgg gtgatcgcca
     1621 tgcttgattc caatgtttat gttagagatc gggtgctctt ccagctttcg cgcaggaagc
     1681 cgccggagta ttgcagcatg attgagcaag aattgaatcg ttttgatccg gattctgcgc
     1741 ctgtgtttcg cgatgcgatt tttgcaatga ctctgtacgg gcagagttgt gttccgaaat
     1801 ctactccacc tggccgattc aaaatctccg ctggcccggg ggatcgcagt ggaagccctg
     1861 actgctttgc ggcaccccga tgcagagaag cttcgcaagc gaaaaccagc caacgatctg
     1921 gagaaagctc gctacagcag cgcgcaaaac atttctggat ttagagatta gcatgaactc
     1981 cggccgggta cgatttctga ttccgattgt tcttttcgca gcgtgtcaat gcgcacggta
     2041 cggcgccaat ggctcttacc tgtccttgct gcttttgcag cgcccggcca gctacaaccc
     2101 cgtgaatcag attcagggag gcctgatcac gatgagttcg gcaatcacgc ctgtggcagt
     2161 caattcagtg gacatgtcgc gttcgatggt cctttgttat tttcgcacaa ctcaatcgga
     2221 tccagcttcc gtgcctacct gcgaattgac ttccagtacg cagatcgtgg ttcagaccgg
     2281 aggaagcagc gtgaactact ttgcgcagtg gtaccttgta gagtttgccg caggagtggt
     2341 ggcccaacga ggaaacacta cgtttgttcc aggtgatttt tcacagagca taacattgcc
     2401 aactgcagtg gacacagcac atagctttgt gctgcttact tctcgcatgg gttcactcaa
     2461 tcagacactc gactctgagc gcacgattcg tggtgtactg agcggcccaa caacattgac
     2521 tctatcgcgc aatgtggcag gtacagcaaa tgtggatgtg cactggcagg tcgtacagct
     2581 ttcggatacc accgtgcaat caggaacact cacaattggt gccggctcat ctacctcaac
     2641 tgctgcgatc agtccggtga atctttcaaa gagctttatt tttttcaata ctcgtgcaga
     2701 cgcggcctcc ggaggatttg aggaagaata tcttgtccgt gcgtcttttg cagacagttc
     2761 aaccgtttcc ttttcgcgcg gtgcttccaa caacacagtc gatgtttctt actttgctgt
     2821 gcaaatgaac gatgcaagtc aggtgcaggc cgggtctgcg gccaccagca gtttcgccgc
     2881 gccaaacaca acgacacaaa tgacttccgc cttgaatgca gtgaatccta acaccacaat
     2941 ccttgccgct tcctcaagtg tgcagaatct tggaggcgga cagcaagcgg atcttgactc
     3001 gggcagctgg gttccgcaga ttgtttcccc aacgctcgtg cagatggatc gtggttcttc
     3061 tcaagaccga ttctcgcagg ttgattattt tgcagttcaa tttgtggcgc agtaagtgca
     3121 tcgccgggat agcgtcagat aaagattctt ctcaaattct cctcgaagcc agcgcctttc
     3181 tcagtttcac gcagtttcct t
//
LOCUS       DAOQJL010000464         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_19812_length_3201_cov_3.686904, whole genome shotgun sequence.
ACCESSION   DAOQJL010000464 DAOQJL010000000
VERSION     DAOQJL010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19812_length_3201_cov_3.686904"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLG78_16295"
     CDS             <1..1464
                     /locus_tag="PLG78_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24734.1"
                     /translation="VRVKQEVLQIREEFQDKTRKIDTSLLEAGRIRARVEEFGERTRD
                     EILSSSGAIIKDMEKKAERFLDEQDQKLSHINQTIDEKISRQIVQLVDRGQLQLDELE
                     KRTEYAIKDAQERMKDQIERARDDFRKMRDDVSREMEVAHAMRDDIMVELEKEKSRMK
                     GLSEKLDVLSRAETLAVKLDETVEVLTDRLKLAEEENSKLDQYVQNFESVRIGRRELE
                     AEIRSLETQRDRIGETEKVIQKMHAQASELKERFGELEQSEAIADRLEERMNDLIEMQ
                     ADFEKSYAEIGEKRKLLEQSLRMMETSKKQAQDARAAAEQLLASVDRAEFRQNEVDKK
                     ILGMESRTVQLSKLEREIDKVESRFMQMEGLMADLEEKQKQIGSMMRKVEEIREHGQE
                     VRTELESTVSEADEKMERLSQLFQTVDRLVDQNFEKAMESSTSVKDRKVKKRSGSDTI
                     KRESILSLHLNHKWDADLIADRLKMDPATVRAIISAS"
     gene            complement(1472..1936)
                     /locus_tag="PLG78_16300"
     CDS             complement(1472..1936)
                     /locus_tag="PLG78_16300"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNE24735.1"
                     /translation="MNFDQNAHPELIGVLGSFTTAKGEFEVRIEGRFDASHYLLKYFP
                     DGSDEPMHGHSWLVEVTLADASGGLREDGLAFDYLAARMRLDKLVDRLDHVVINHLPE
                     FKGINPTTENIARWFYAGLRSEVEGKGGRVIGIRVHEGPANSALFRPSHNQS"
     gene            2029..2250
                     /locus_tag="PLG78_16305"
     CDS             2029..2250
                     /locus_tag="PLG78_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HNE24736.1"
                     /translation="MSDSATKQRFTASMTVGEAMTLHPEAGLVFSSYHLGGCSHCSIN
                     ELETIEQVCAGYGVPVEQLVDSLNNLLDS"
     gene            2494..3105
                     /locus_tag="PLG78_16310"
     CDS             2494..3105
                     /locus_tag="PLG78_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24737.1"
                     /translation="MRLQTIAILFFFAAAATGCNKDSGESSEGRGVYSLSSVEGPDKV
                     KALITNDFLKWINDPVIVEAIKKANAKNEKRSMDEIKAADQEWIKAEGISDLMREMLT
                     NDAAQFLKKKQKESDGKYSEIFVMDFQGCNVAMSAKTSDFWQGDEAKFIKSYNDGKGA
                     VFVDKVKFDESTKVPQVQVSLPVLDGNKVIGAITIGVSLDNLQ"
BASE COUNT          940 a          736 c          846 g          679 t
ORIGIN      
        1 gttcgagtga aacaggaagt ccttcaaatt cgtgaagagt tccaggacaa aacgcgcaag
       61 atcgacacgt ctctccttga ggcaggaaga attcgggcgc gagtggaaga gttcggagaa
      121 agaactcgcg atgaaatcct ttcttcttcc ggtgcaatca tcaaagacat ggaaaaaaag
      181 gcagagaggt tcctcgacga acaggaccag aaactcagcc acatcaatca gaccatcgac
      241 gaaaagattt cgcgtcagat tgtgcagctt gtagaccgcg gccagctcca gctggacgaa
      301 cttgaaaaga gaacagaata cgcaatcaaa gacgctcaag agcgcatgaa agatcagatc
      361 gagcgagcgc gggacgattt ccgcaaaatg cgagatgacg tgagccgcga gatggaagta
      421 gcgcatgcaa tgcgtgatga catcatggtg gaacttgaga aagaaaagag ccgcatgaaa
      481 ggcctttcag agaagctgga tgttctttcg agagcggaga cgcttgccgt caaactggat
      541 gagactgtag aagttctcac agatcgtttg aaactagcag aagaagagaa ctccaaactc
      601 gatcagtacg tgcagaactt cgaatctgta agaattggtc gtcgggaact cgaggcggag
      661 atcagaagcc ttgagaccca gcgagacaga attggagaaa cagaaaaagt aatccagaaa
      721 atgcatgctc aggcatccga gctcaaagag cgctttggtg aactggagca atcagaggca
      781 attgcggatc gtctggaaga aagaatgaac gatctcatcg agatgcaggc ggactttgaa
      841 aaatcctacg cagaaatcgg agaaaagcgc aagctcctcg aacaatctct gcgcatgatg
      901 gaaacatcca agaaacaggc ccaggacgcg cgagcagctg cagaacaatt gctggccagc
      961 gtggatcgtg cagaattccg tcagaacgaa gtcgacaaaa agattctggg aatggagtct
     1021 cgcacggttc aactttccaa actcgagcgc gaaatcgaca aggtagagtc tcgctttatg
     1081 caaatggaag gtctgatggc agacctggaa gaaaagcaga agcagatcgg ttcgatgatg
     1141 cgcaaggtgg aagagattcg agaacatggt caggaagtga ggaccgaact ggaatccaca
     1201 gtgagcgaag cggatgaaaa aatggaacgg ttgagccagc tcttccagac tgtggaccgg
     1261 cttgtagatc aaaacttcga aaaggcaatg gaatcaagca ccagcgtaaa ggacagaaag
     1321 gtcaagaaac gcagcggttc agatacaatc aaacgcgaaa gcatcctgag cctgcaccta
     1381 aaccacaagt gggatgcgga tctcatcgcg gatcgtttga agatggatcc ggcgactgtt
     1441 cgcgccatca tcagcgcgtc ctgacgctcg cttagctctg gttatgcgaa gggcgaaaca
     1501 gggccgaatt ggcggggcct tcgtgcacac gaatgccaat gacgcggcct cctttgccct
     1561 ccacctctga gcgtaatccc gcgtaaaacc agcgggctat gttttcggtg gttggattga
     1621 tccctttgaa ctcgggtaga tgattgatga ctacgtgatc gagcctgtct acaagcttgt
     1681 ccaatctcat cctggcggcc agataatcaa aggccaggcc gtcttcgcgc agaccaccgc
     1741 tcgcgtccgc cagtgtgacc tctacaagcc aggagtgtcc gtgcatgggt tcgtcggaac
     1801 catccgggaa atatttaagc aggtagtggc tggcgtcgaa cctgccttcg atgcgaactt
     1861 caaattcgcc cttcgcggtc gtaaacgaac ccagcacacc gatcagttcg gggtgcgcgt
     1921 tttgatcgaa gttcatgcct tacccaactc aaaataagag ttgaccgtcc aggttttttt
     1981 cgactttatt ctagagcgga acaaaattca acaacaggag gtgatccaat gtctgattct
     2041 gcaacaaaac aaaggtttac ggcaagtatg acggtggggg aagctatgac attgcatccc
     2101 gaagcaggtc tggttttctc aagctatcat ctcggtggtt gctcgcattg ttccatcaat
     2161 gagcttgaaa ctatcgagca ggtttgcgct ggttatggcg ttcctgtaga acagctcgtc
     2221 gacagcctca acaaccttct ggacagttga tgcagcaaga gccgggtatc gcccggctct
     2281 tgaactcaat ttttgtgtcc agggtttcac gatgaaactt catcgccgca atttccatca
     2341 tcaagcaaaa gttctcagta gtcattctca ccttttcgct ggcccttcac gtagcttagc
     2401 agaactttgt tgcaaagaat cacgcccggc attgattcaa cacatcggtt ttcgcgtggt
     2461 tgaacaaatt catttcgcag ggaaaagcaa actatgagac tccaaacaat cgcaattctc
     2521 ttttttttcg cagcggcagc caccggctgc aacaaagact ctggtgaaag ctcggaaggc
     2581 agaggcgtat acagcctgtc aagcgtcgaa gggccagaca aagtcaaggc cttgatcaca
     2641 aacgatttcc tcaagtggat caacgatcct gtaatcgtag aagcaatcaa aaaggcgaat
     2701 gcgaagaatg aaaagagatc tatggatgaa atcaaagcag cggatcagga atggattaaa
     2761 gcagaaggga tttcagattt gatgagggag atgctgacca atgatgcagc tcagtttctg
     2821 aaaaagaagc agaaggaatc ggatggcaaa tacagcgaga tctttgtcat ggattttcag
     2881 ggatgcaacg tggccatgag tgcgaagaca tctgatttct ggcagggcga cgaagccaaa
     2941 ttcattaagt cctacaacga tggcaaaggc gcagtcttcg tagacaaagt caagttcgac
     3001 gaaagtacga aagtcccaca ggtgcaggtt tcgctgccag tcctggatgg caacaaagtg
     3061 attggtgcaa ttactattgg tgtttctctc gacaacctgc aataacgtcc gggaatttcg
     3121 caaaacatcc tgaaccacat gtttgccaag atgaagattt ccaccagaat cgttctgggt
     3181 ttcgcggcga tcttgcttct a
//
LOCUS       DAOQJL010000465         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20023_length_3180_cov_4.151040, whole genome shotgun sequence.
ACCESSION   DAOQJL010000465 DAOQJL010000000
VERSION     DAOQJL010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20023_length_3180_cov_4.151040"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLG78_16315"
     CDS             complement(<1..756)
                     /locus_tag="PLG78_16315"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNE24738.1"
                     /translation="MTLCSRQFLLLLVFVSTSTFADELADRIAAVPNPRTTGGWVSDP
                     SQVLATRTDAINHLISDHEKETTDEIAVVVLPSIGQLVPKEFATALFNSWKIGKAKKN
                     NGVLVLHILDQRRIEIETGYGQEGPLPDVKCKWILDEVVVPYFKKGSFADGHYEGVRA
                     ILRALKDPDIKHDDLIAGTETTPGDSIQELPKIPERDEITIKNMGTVQRSIYDGTAPK
                     LLSAGGGGLFVLGYLLYWLFGLGKDPYKKYKLFE"
     gene            complement(753..1706)
                     /locus_tag="PLG78_16320"
     CDS             complement(753..1706)
                     /locus_tag="PLG78_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24739.1"
                     /translation="MKVKFWGVRGSIGSPVKGHALRDKVRRILQYASPADILDDEAID
                     RFLKSLPFSLLQTYGGNTTCLEIRSDKNDLVIVDAGTGIRELGMKMLSEGFLQGKGEC
                     AMVFTHTHWDHIQGLPFFAPFYIPGNKFEVHAISENLEDRLKYQHSSRHFPVGFEEMR
                     ATRSFVQHSEAEEWPLYGMKVRTKGMRHPGNSYSYRFEESGKSIIFGSDAEFKLEDMD
                     RIDEYMDYFRDADVLIFDTQYTFEEQLQKIDWGHSSASIATDIALRSGVKKLVLFHHD
                     PSYDDEKLDEVYLRALRYREMMDKQSKLEIVMAYEGLEIAV"
     gene            complement(1741..2796)
                     /locus_tag="PLG78_16325"
     CDS             complement(1741..2796)
                     /locus_tag="PLG78_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24740.1"
                     /translation="MTTPKEIKDQAEFLKMIGSTDLERPEIVLISGDDVEAVDSALDR
                     MKHRVLSRHKDCLVPVFGNEPDDDNRFLTEIENVPLFSPFRLIVVRDAQAVFASILKQ
                     KDRKSDFAHFISSLPDRTWILVHYSGRATDDFLSLWGSHAIHLATKDLYPEQVIDYIR
                     NASRRAGLSLAEEAVLEIRDRIVHRPGAIDAACARIRDHLPAGERNASLDFVQEVLFP
                     YPGWNLSTLVDSLFAGDSARFFSEIAKRDPSDDYYRPMKAVLNRTDELRRARIALDHG
                     LSDKDVMMFTGHGNKHPYVQKKILQRLRYETERFSNERILSIYDALDSLFKSFRRGTD
                     RDQQEILFTEKVASIFF"
     gene            2823..>3180
                     /locus_tag="PLG78_16330"
     CDS             2823..>3180
                     /locus_tag="PLG78_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE24741.1"
                     /translation="MAVFAMKKSGSKLLVELLQSKGVDLVFGYPGGAILPFYDELYHS
                     KIKHILVRHEQGAMHMAEGYARATGRPGVVIVTSGPGATNTITGLCDAKLDSIPLMVI
                     TGQVATTAIGTDAFQEA"
BASE COUNT          795 a          791 c          753 g          841 t
ORIGIN      
        1 ttcaaacaac ttgtattttt tatatggatc ttttccgagt ccaaagagcc agtaaagtag
       61 ataacccagc acaaacaatc ctccgccccc ggcggaaagg agtttaggag ctgtcccatc
      121 gtagatggat ctctggacgg tccccatgtt cttgattgtg atttcatctc tttctggaat
      181 ctttgggagc tcctgaatgc tgtctcctgg agtggtttct gttcccgcaa tcaaatcgtc
      241 gtgcttgatg tccgggtctt tgagcgcgcg aagtatggct cgaactcctt cgtaatgacc
      301 gtctgcgaag cttccctttt taaaataggg cacaaccact tcgtccagaa tccatttgca
      361 tttcacatct ggaagcggac cttcctgacc gtagcccgtt tcgatttcaa tccgcctctg
      421 gtcgagtatg tggagcacca gtacgccatt gttctttttc gcttttccaa tcttccagct
      481 attgaagaga gcagttgcga attcttttgg aacgagctgg cctattgaag gcaatactac
      541 gactgcgatt tcgtctgtgg tttctttttc gtgatcagag atcagatgat tgatggcgtc
      601 cgttcttgtt gcaagaacct gagaaggatc tgaaacccat ccacctgttg tgcgcggatt
      661 gggaacggca gcgatccgat ctgcgagctc gtcagcaaaa gtagaagtag aaacaaaaac
      721 aagcagaagg aggaactgtc tagagcaaag agtcataccg caatttcaag tccttcgtaa
      781 gccattacga tttctagttt tgattgtttg tccatcattt cgcgatagcg aagagcgcga
      841 agatacactt catctaactt ttcgtcatcg taagatggat catgatggaa tagcaccagt
      901 ttcttcactc cagatctcag agcgatgtct gttgcgatgg acgcagaact atgcccccag
      961 tcgattttct gaagctgttc ttcgaatgtg tattgtgtat caaaaatgag aacatccgcg
     1021 tcccgaaagt aatccatgta ttcatcaata cgatccatgt cttcgagctt aaattccgca
     1081 tcgcttccga agatgatgga cttaccgctt tcttcaaacc tgtacgaata gctattgccg
     1141 ggatggcgca ttccctttgt gcgaaccttc attccataaa gtggccattc ctctgcttcc
     1201 gaatgctgca caaaagaacg agtggctctc atctcttcga atccaaccgg aaagtgtctg
     1261 ctggaatgct ggtatttgag tctgtcctct agattctctg aaatggcatg cacttcgaac
     1321 ttgtttcctg gaatataaaa aggtgcgaag aacggaagtc cctgaatgtg atcccagtga
     1381 gtgtgcgtaa agaccatcgc acattctcct ttgccctgga gaaaaccttc tgaaagcatt
     1441 ttcattccaa gctcgcgaat gcctgtcccc gcatccacga tcaccagatc gtttttatct
     1501 gaacgaattt ccaggcacgt tgtatttccg ccgtaggttt gcagaagaga aaatggaagt
     1561 gatttcaaga atcgatcaat tgcttcgtcg tccaggatat ccgccggaga agcatactgc
     1621 agaattctcc gcactttgtc gcgcagggcg tgacctttta caggggaccc gatggatcca
     1681 cgaacgcccc agaacttcac cttcatattc ccaggataaa aaacgggcag gggctgccgt
     1741 ctagaagaaa atagaagcca ctttttcagt aaaaagaatt tcctgctggt ctctgtccgt
     1801 tccccggcgg aaactcttaa aaagggaatc aagcgcatcg taaatactca ggattctctc
     1861 atttgaaaat cgttctgttt catagcgcag acgttgcagg atcttcttct gcacgtacgg
     1921 gtgtttgttg ccgtgaccgg taaacatcat cacatctttg tcgctgagcc catggtccag
     1981 ggcaatgcga gctcttctca gctcatcggt tctgttgagc actgctttca tgggtcgata
     2041 gtagtcgtct gacggatctc tctttgcaat ctccgaaaaa aaacgcgcgg agtctcccgc
     2101 gaaaagagaa tctaccagag tgctcaaatt ccagcctgga taaggaaata aaacttcctg
     2161 aacaaaatca agagatgcgt ttctttcccc ggcgggcaaa tgatcgcgaa tccgggcgca
     2221 cgcagcgtca atcgctcctg gcctgtgcac gattctgtct ctgatctcta agactgcttc
     2281 ttccgcaagg ctcagacccg ctcgcctgga tgcgtttcga atgtagtcga tcacctgttc
     2341 cggatagaga tcctttgttg cgaggtggat ggcatgcgat ccccacagag acagaaaatc
     2401 gtctgtggcc cgaccgctgt aatgaacgag aatccatgtg cgatccggca gagacgaaat
     2461 gaaatgagca aaatctgatt tgcgatcctt ctgcttcagt atggaagcga acacagcctg
     2521 ggcgtctctg actacgatga gacggaatgg agagaagagc ggaacgtttt cgatttctgt
     2581 cagaaaccga ttgtcgtcat ctggctcatt tccgaacacg ggcacaagac agtctttatg
     2641 cctggaaaga actctgtgct tcatcctgtc cagggcggag tccacagctt ccacgtcgtc
     2701 gcccgaaatc agcacaattt caggcctttc caggtccgtg ctgccgatca tcttgagaaa
     2761 ttcggcctga tccttaatct cttttggagt tgtcatggag ggtcgttctc gaatttatag
     2821 aggtggcggt atttgcaatg aagaaaagcg gatccaaact gctcgttgag cttttacaat
     2881 ccaaaggggt ggacctggtg ttcggttatc caggtggagc catccttcct ttctatgacg
     2941 aactctatca ttctaaaatc aaacacatcc tggtgaggca cgaacagggc gccatgcata
     3001 tggcagaagg ttacgcaaga gccacaggca gacctggagt tgtcattgta acaagcggac
     3061 ccggagcgac caacacaatc acaggactct gcgatgcgaa gctggattcc attccactca
     3121 tggtgatcac cggccaggta gccaccacag ccattggcac agacgcattt caggaagcgg
//
LOCUS       DAOQJL010000466         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20164_length_3167_cov_5.210154, whole genome shotgun sequence.
ACCESSION   DAOQJL010000466 DAOQJL010000000
VERSION     DAOQJL010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20164_length_3167_cov_5.210154"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLG78_16335"
     CDS             complement(<1..450)
                     /locus_tag="PLG78_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE24742.1"
                     /translation="MNRAKLTKIIATLGPASSSEDAIRSLIRAGADCFRLNFSHADGP
                     AMQPLIDRVRAVSQSEGVPIAILADIQGPKLRIGKLADEGVLLVEGKTFTITTREITG
                     TDQIVHSPYEFLTRDVKPGSRLLLADGTIELLVENVTSTDVNARIVRG"
     gene            complement(507..1448)
                     /locus_tag="PLG78_16340"
     CDS             complement(507..1448)
                     /locus_tag="PLG78_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE24743.1"
                     /translation="MRAIAYKKYGKAEVLEEMDLPDPVMGEDDLLVRIHAVSVNPIDW
                     KIRSGALRLLSGGNFPRIPGSDFAGEVLSTGKNTRRFQKGDRVFGMLPPMKGGAFATL
                     AAVPERLAAQAPRNLTYEECAAVPLAGLTALQALRDPGEIQDSQKVLIYGAAGGVGTF
                     AIQIARIFQADVTAVASGSSEEIVRSAGANRFIDYTRENFARIDSKYDIVFDCVGKTY
                     YADCVDILKPDGKYITVELGPGVFWDSLRGGFFGGPTCSFIITKESSDDLDMLRHYIQ
                     GKTIKPVISTIFPLSRAREAHALSEAGHVKGKIVLRM"
     gene            1547..2449
                     /locus_tag="PLG78_16345"
     CDS             1547..2449
                     /locus_tag="PLG78_16345"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE24744.1"
                     /translation="MLVRFFFLGLLLNSSCALLVRKGSVAEIQQIAVQGQVRRYILHV
                     PASYDAQKKTALVVVLHGGGGNGRNAEKMSGFSAKADKEGFIVVYPYGSGRLDERLLT
                     WNAGNCCGYALDKNVDDVAFIRELVVLLKRQYSIDDKQIFATGMSNGGMMTHRLACEM
                     SDVFSGVAPVSGAMNVPVCNPARAVSMVIFHGKKDEHVLFEGGAPKKSVDSHPRVDKS
                     VAAAVEFWKNRNGCAQSPTESHGKIQIEDFSCSQAGLAVYAIEDEGHTWPGGEKGYFA
                     ADEPTRELSATDVMWDFWQKNSRP"
     gene            complement(2412..2600)
                     /locus_tag="PLG78_16350"
     CDS             complement(2412..2600)
                     /locus_tag="PLG78_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24745.1"
                     /translation="MMERNLKSSTEIKRKHGRGIVLTRAMFDSVNYTSPGNEVRLTRK
                     FDASPSITDANSSARNPT"
     gene            complement(2654..>3167)
                     /locus_tag="PLG78_16355"
     CDS             complement(2654..>3167)
                     /locus_tag="PLG78_16355"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNE24746.1"
                     /translation="LELKVPHGSDLTQLLPLVPVFLSYVLSFLYLGIYWNNHHHMLHL
                     ANRINGKVLWANMHLLFWLSLIPFVTAWSGENHFETNTVVAYAFVLLMCGAAYYILEQ
                     MLIAAEGKDSALAKAVGNDRKGIASLVLYILSIAIAFYNSQAAMAVIVIVAIMWIVPD
                     TRIEKHFNKR"
BASE COUNT          737 a          781 c          817 g          832 t
ORIGIN      
        1 tccgcgaaca atgcgcgcgt ttacatctgt ggatgttacg ttttccacaa gtagttcaat
       61 ggtcccgtct gcgaggagca atctcgaacc gggcttcacg tctctggtaa ggaattcata
      121 ggggctatga acgatttgat ctgtgcctgt gatttcgcgc gttgtaatgg tgaatgtttt
      181 tccttctaca aggaggactc cttcgtctgc gagctttcca atgcgcagct ttggaccttg
      241 aatatccgcc agaattgcaa tgggcacacc ttctgactga gagaccgctc gcacgcgatc
      301 aatgagcggt tgcatggcag ggccatccgc gtgagaaaaa ttaagccgaa agcaatccgc
      361 acccgcgcgg atgagagagc gaatggcatc ttcggaagag gaagccggtc caagagttgc
      421 gatgatcttt gttaattttg cacgattcat aagaattctt ttcgaacact cctttatttc
      481 acaatcgtaa tctggcgggc ggctgactac attcttagca caatctttcc tttcacatgc
      541 cccgcttcgc tcagtgcgtg agcttctcgc gctcgcgaaa gcggaaagat cgtgctgatc
      601 acaggcttga tggtctttcc ttgtatgtaa tgccggagca tgtccagatc atcactggat
      661 tcttttgtaa tgatgaagct gcaagtaggt cctccaaaaa atccgcctct tagagaatcc
      721 cagaaaacgc ctggaccaag ttcgaccgtt atgtactttc catcaggctt aagaatatcc
      781 acgcagtctg cataatacgt ttttccaacg caatcaaaaa ctatgtcata cttactatcg
      841 attcgcgcaa agttttcgcg cgtgtagtca atgaatctgt tcgcgccagc ggatcgcaca
      901 atctcttcgc ttgagccact ggccactgcc gttacatccg cctggaaaat gcgcgcgatc
      961 tgaatggcga aagttcccac tccgccggca gcgccataga tcagtacctt ctggctgtct
     1021 tgaatttctc ctggatcgcg aagagcctga agcgctgtca aaccggcaag agggacagcg
     1081 gcgcattctt cgtaagtcag gtttcgcgga gcctgcgccg ccagtctctc cgggacagct
     1141 gcaagggtcg cgaaagcgcc tcctttcatg ggcggaagca tcccgaagac tctatcgcct
     1201 ttctgaaatc gtcttgtgtt cttgcctgtg gaaagtactt ccccggcaaa atcagatccc
     1261 gggatccgtg gaaaatttcc gccgctgaga agcctgagcg ctcctgatcg gatcttccag
     1321 tcgattggat tgactgacac tgcgtgaatc cggacaagca ggtcatcctc tcccatgaca
     1381 ggatccggta gatccatctc ttccaggact tctgccttgc cgtatttctt gtacgcaatg
     1441 gcgcgcattt tcagatttcg tctctccctc taattccggt caattcgttc tccactcgtt
     1501 ttgattttta gttggatttt ttcccgttat ccggcaatga gacagcatgc tcgtgcgatt
     1561 tttctttctg ggcctgctct tgaattcaag ctgcgccctt cttgttcgca agggttcggt
     1621 ggcggagatc caacagattg cagtgcaggg gcaggtgcga cgctacattt tgcatgtgcc
     1681 tgcttcctac gatgcgcaga agaagactgc tcttgtggtt gtgcttcatg gcggcggcgg
     1741 aaacggacga aacgcggaga aaatgagcgg tttcagcgcg aaggcggaca aagaaggctt
     1801 cattgtggtc tatccatacg gaagcggacg cctcgatgaa aggctgctca cctggaatgc
     1861 aggaaactgc tgtggatacg cgctcgacaa gaatgtggat gatgttgcgt tcattcgcga
     1921 gcttgtcgtt ttgttaaaga gacagtactc gattgatgac aaacagatct tcgctaccgg
     1981 catgtccaac ggtggcatga tgactcatcg ccttgcgtgc gaaatgtcgg acgttttttc
     2041 tggagttgct ccagtttctg gggcgatgaa cgttcctgtg tgcaatccag cgcgtgcggt
     2101 ttctatggtg atctttcatg gcaaaaagga tgagcatgta ttgtttgagg ggggcgctcc
     2161 aaaaaaaagt gtggattcgc atcctcgcgt ggacaaatcc gtggctgctg cggtagaatt
     2221 ttggaagaac aggaatggct gtgcgcaatc gccgactgaa tcgcatggaa agattcagat
     2281 agaagatttt tcctgctcgc aggcggggct ggcagtatat gcgatcgaag acgaagggca
     2341 tacctggccc ggtggtgaga aaggctactt tgctgcggat gaaccaacgc gtgaactatc
     2401 tgccacagac gttatgtggg atttctggca gaagaattcg cgtccgtaat ggagggtgaa
     2461 gcgtcgaact tgcgcgtcag gcgaacttca tttcctggag aagtataatt cacagaatca
     2521 aacatggctc gcgttaaaac aattcctctt ccatgctttc gtttgatctc cgtgctgctc
     2581 ttgagattgc gctccatcaa agccctttca gtctctcctc ttcattctgc gggatcggag
     2641 ctgaaatcat acctcacctc ttattgaaat gcttttcaat tcttgtgtcc ggcacgatcc
     2701 acataatcgc cacgatcacg atcactgcca tggcagcctg tgagttatag aatgcaatcg
     2761 caatggagag aatataaagc accagagaag caattccttt gcggtcgttt cctacagcct
     2821 ttgcgagagc tgagtctttt ccctctgcgg cgatcagcat ctgttctaga atatagtaag
     2881 ctgctccgca cataagcaga acgaatgcgt aagcgacaac cgtattggtt tcaaaatgat
     2941 tttctccgct ccaggctgtc acaaaaggga tgagcgaaag ccagaagaga agatgcatgt
     3001 tggcccatag tactttgccg ttgattctat tcgcaaggtg cagcatgtga tggtgattgt
     3061 tccagtagat tccaaggtaa agaaagctca ggacgtagct caggaataca ggtacaagtg
     3121 gaagtagctg cgtcagatcg ctgccgtgcg gcaccttcag ttccagt
//
LOCUS       DAOQJL010000467         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20177_length_3166_cov_4.490518, whole genome shotgun sequence.
ACCESSION   DAOQJL010000467 DAOQJL010000000
VERSION     DAOQJL010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20177_length_3166_cov_4.490518"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLG78_16360"
     CDS             complement(<1..1057)
                     /locus_tag="PLG78_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE24747.1"
                     /translation="MARSLQFIGGTGTVTGSKYLITTKNNRILVDCGLFQGLKVLRER
                     NRKPLPLSPRSISHVVLTHAHLDHSGYVPLLVKKGYSGEILSTRATKALCGILLPDSG
                     RLQEEDAHYANKHQFTKHEPALPLYTEKDARHCLKYFRTVEYHQNIKLSKNISFEYFR
                     AGHIPGSCIVKFNLGGETVVFSGDLGRPINNLLPQPDRVTDADVLVLESTYGDRVHDR
                     EDPEVLLERLINKTVQRKGRILIPAFAVGRTQEILHFVIRLKKQNRIPQIPIYVDSPM
                     ATEATRVFLSFSEENRLTAEEAADMRAHARFVDTTEESRALNDSRESAIIISASGMAT
                     GGRVLHHLEHMADVPENM"
     gene            complement(1057..2706)
                     /locus_tag="PLG78_16365"
     CDS             complement(1057..2706)
                     /locus_tag="PLG78_16365"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNE24748.1"
                     /translation="MEDIEALFDSQNGHFIVDSENGRAMLTVHSPGKKGMPVRLTDVL
                     ARLELFRVSGFSKNVLEAIVQDADGMPHDVGIFPEPDAIAARVEIELSPDKMQAYAVI
                     HPPVHGGRWVLESELSSAMEAAHIVHGVRAEWKTQALTPVSMDGPIRSLIAQGTPVQM
                     GVPATVEYGVDPRPGLAPAADGETRVDFRKIHVIQTCEEGKILARIFKGEPGHAGVTV
                     TGEVKDPPELLVMRLVAGINTRLSADGSVIEAEKSGHVRLREIEMPGEIRLHFDVDDV
                     LKLETVDFSTGHIDFPGTVIVTGVVADGFDIVASGDVILEKTIGAVHVRAGGSIFLSE
                     GVIGRGAASLQAGGQILSRFVQSAALHAGGSIVIEDAVLHSRLVSGKDISVEQGKGEI
                     LGGEAICSGIFRANRIGSRLETPTRIVLGLDPDTLEKLRALDRDIYEKKRILDRVESH
                     LKQLEEARQRRKEVDLDTESKLLELREKQSSIVNALEKQRRKLQDRIAPNPKSALHTL
                     EAHPGVEVYFGVGIAPYRIFGRPVRNLKLTLADGKIHAGKI"
     gene            complement(2706..>3166)
                     /locus_tag="PLG78_16370"
     CDS             complement(2706..>3166)
                     /locus_tag="PLG78_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24749.1"
                     /translation="DVRFADVLSRMGAVVTRLDDSITVKRGNLRGIDIDMNDMPDAAM
                     TLAVLALFAEGPTHIRNIANLRVKESERIAGLASELRKLGAIVEEGHDNLKIVPPARI
                     QSAVIETFQDHRMAMAFSLASFGADVTILDPMCVRKTYPAYFEDFKRVFG"
BASE COUNT          748 a          826 c          764 g          828 t
ORIGIN      
        1 tcatgttctc aggaacatca gccatgtgtt ctagatgatg cagtacgcga ccacccgtag
       61 ccattccgct tgcagaaatg atgatagcag actctctgct gtcgttgagg gctctcgatt
      121 cttctgttgt atctacaaaa cgcgcgtgcg cgcgcatatc agcggcttct tcagccgtca
      181 ggcgattttc ctcggaaaaa gacaaaaaca cccgggttgc ttctgttgcc atggggctat
      241 ccacgtaaat cgggatctgg ggaattcgat tttgtttttt gaggcgaatg acaaagtgaa
      301 gaatttcctg agtgcgtccc actgcgaaag ccgggattaa gattctacct ttacgctgta
      361 cggtcttatt gatcaagcgc tcgagcaata cttcggggtc ttctctgtca tgaacgcgat
      421 ccccgtaggt ggattcgaga accagcacgt ccgcatctgt tacacgatct ggctgtggca
      481 gaagattgtt gatggggcga ccaagatctc ccgaaaatac cacagtttct ccgcccagat
      541 tgaattttac aatgcatgag ccggggatgt gtcccgcacg aaagtattca aatgatatgt
      601 ttttacttag ttttatattc tgatgatact ctacagttcg aaaatacttc aagcaatgac
      661 gagcatcttt ctccgtatac aatggaagag caggctcatg ttttgtgaac tgatgcttgt
      721 ttgcgtagtg agcatcctct tcctgaagac gaccggaatc tggaagtaga atgccgcata
      781 acgctttggt tgcacgcgta gagagaatct ccccgctgta gcccttctta acaagaagag
      841 gtacataccc gctatgatcg agatgcgcgt gggtgaggac aacatggctg atgctgcgcg
      901 gagatagcgg aagaggctta cggtttcttt cgcgaagtac cttgaggccc tgaaacaggc
      961 cacaatccac gagtatcctg ttgttcttcg tagtaatcaa atacttggat ccagtgactg
     1021 ttcctgtgcc accaatgaat tgaagagaac gagccattat atttttcctg cgtggatttt
     1081 tccgtccgcg agagtgagtt tcaaattgcg aacgggtctt ccaaaaatgc gatagggtgc
     1141 gattcccact ccgaagtata cttccacgcc cggatgagct tccagggtat gcagcgcgga
     1201 ttttggattg ggagcgattc gatcctggag cttgcgtctc tgtttttcga gcgcatttac
     1261 aatgctcgat tgtttttcac ggagttcgag gagtttggat tctgtgtcca gatccacttc
     1321 ctttcttctc tggcgagctt cttccagttg cttgagatgg ctttccaccc ggtcgaggat
     1381 tcttttcttt tcgtagatat ctcgatccag ggcccgcaat ttctcgagcg tatccggatc
     1441 cagcccaaga acgattctgg tgggtgtttc cagtctggac ccgattctat ttgcgcgaaa
     1501 gattccagaa cagattgctt ctccacccag gatttcacct ttgccctgtt ccacggaaat
     1561 gtctttaccc gatacaagcc tcgaatgcaa gacagcatcc tcaatcacaa tggacccgcc
     1621 tgcatgaagt gccgcagact gaacaaaacg cgaaaggatc tgtccgcccg cctgaaggct
     1681 ggcagctcct ctgccaatga ctccttcaga taagaagatg gaaccgccgg ctcgcacgtg
     1741 cacggctcca atcgtttttt caaggatcac atctccagaa gcaacaatgt caaatccatc
     1801 cgcgacgact ccagtaacta tgactgtccc gggaaaatcg atgtgccctg tggaaaaatc
     1861 gacggtttcg agtttgagaa catcatccac atcgaaatgg agtctgatct ctcctggcat
     1921 ttctatttcg cgcaaacgaa catgaccgct cttctctgct tcgatcacgg aaccatctgc
     1981 agacaaacgc gtgttgattc ccgcgaccaa tcgcatcacc aggagttccg gtggatcctt
     2041 cacctctcct gtcacagtca cacctgcatg gcctggctcc cctttgaaaa tacgtgcgag
     2101 tatttttcct tcttcgcaag tctggatcac gtgaatcttg cgaaagtcca ctctggtttc
     2161 cccgtctgca gcaggagcaa gaccaggcct gggatccact ccgtattcaa ctgttgcagg
     2221 gactcccatc tgcacgggtg tgccctgcgc gatcaaggat cggattggtc catccatgga
     2281 tactggcgtg agagcctggg ttttccattc tgcgcgaact ccatgcacaa tatgcgcggc
     2341 ttccatagcg gaagaaagct ctgattctaa aacccagcgg cctccgtgga caggaggatg
     2401 aatcactgcg tatgcttgca ttttatcggg ggaaagctct atctccactc gtgcggcgat
     2461 cgcgtccggt tcggggaaaa ttccaacgtc atgcggcatt ccgtccgcat cttgcacaat
     2521 cgcttcgagc acattttttg agaatcctga aacacgaaac aattcaagtc tggcaagaac
     2581 gtccgtgagt ctcactggca tccctttttt tcctggagag tgcacagtga gcatggcccg
     2641 tccattctcc gagtctacga tgaagtgacc gttctgagaa tcaaaaagcg cttctatgtc
     2701 ttccattacc cgaacactct cttgaaatct tcgaaatagg caggatatgt ttttcgcacg
     2761 cacatgggat cgagaatggt aacgtccgca ccaaaagagg ccagagaaaa cgccatagcc
     2821 attctgtggt cctgaaatgt ttcgatgact gcagattgaa tgcgagcagg cggaacgatc
     2881 ttcaggttgt cgtgcccctc ttccacaatg gcacccagct ttctaagttc agacgccagc
     2941 ccggcaattc tctcggattc ttttacacga aggttggcaa tgttgcgaat gtgagtaggt
     3001 ccttctgcaa atagggcaag aacggcaaga gtcatggccg catccggcat gtcgttcatg
     3061 tctatgtcta tgccgcgcaa gtttccgcgc ttgactgtaa tggaatcatc taaacgagtt
     3121 accaccgcgc ccatccgcga gagcacatct gcaaatcgaa catctc
//
LOCUS       DAOQJL010000468         3160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20250_length_3160_cov_3.526892, whole genome shotgun sequence.
ACCESSION   DAOQJL010000468 DAOQJL010000000
VERSION     DAOQJL010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20250_length_3160_cov_3.526892"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLG78_16375"
     CDS             <1..397
                     /locus_tag="PLG78_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNE24750.1"
                     /translation="FAQGISNSVFRFMKLATPGPFTFLFKANRNMDRRATGKRKEVGI
                     RIVDHAVVQAIIEELGGPLMSTSVPEEEYFIDPEHLDAVWGKRVHAIVDGGVKENTGS
                     SILDCADGEIKLVRKGAGAEILAQLGLEE"
     gene            440..1165
                     /locus_tag="PLG78_16380"
     CDS             440..1165
                     /locus_tag="PLG78_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE24751.1"
                     /translation="MSGHSRWANIKHRKEGADKKRGVAFAKISKELIVAARLGGSDPA
                     SNARLRISISKARAANMPRDNIERAVKKGAGELEGTTYEEILYEVYAPGGVGILVEGL
                     TDKKSRTTPEIKSILTKYGGNLAMEGAVSRNFTRCGVIVIESQGKSEDEWMEFALDAG
                     ADDFVVQEGFFEIRVPVDRYAALQEKLSQKEASVVESGIKFVPLPGTEVEVKDADQAA
                     KIQKFLDALEDHDDIQAVSTNWA"
     gene            1165..1665
                     /locus_tag="PLG78_16385"
     CDS             1165..1665
                     /locus_tag="PLG78_16385"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNE24752.1"
                     /translation="MRILAFDPGYGITGWGVVEEPPLLAVDYGAIETSARDKHEVRLL
                     EILNTSRELIGRFKPSFIVVETLFVSKNAKTAGGVYESRGVILAACAESGTEVIELGP
                     TTIKKTVTGSGQATKQSVHQMVKRLLGISQNIRPDDTADALAGAIAGILAAKSRMVKQ
                     RIRSAK"
     gene            1662..2252
                     /gene="ruvA"
                     /locus_tag="PLG78_16390"
     CDS             1662..2252
                     /gene="ruvA"
                     /locus_tag="PLG78_16390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNE24753.1"
                     /translation="MISGIQGTLVRLGASSAFVLAGGLEYEVYVPLSVYDALEKQGSG
                     SDVHLFVHHHFSQEDQKLFGFADASEREVFRELIRLQGMGPALALSVLSHYDGPRLLS
                     SCESGDVQGLTQIPRVGKKTAERLIFEITSRKERFRKLLAGQRKGPAPETEQDLAREA
                     LLQLGYREAAIAEAFKKLDAKPRTASEWITSSLRVL"
     gene            2312..>3160
                     /locus_tag="PLG78_16395"
     CDS             2312..>3160
                     /locus_tag="PLG78_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24754.1"
                     /translation="MATLASIRKYLYPARYQLAAVLAAAVFLSALAIWKYRQDMQGAL
                     HNYRLEAHDEALRVARNANDMFRLIYQGLRTIARLPGVRKIQDQNLVFRGGGAGFEAD
                     AKLAVQELYNNLATNVAISEVYIAPRDLDPDAPAYSNAHKEPLVTFDELIVGQTALGN
                     KGDNAESVPEIELYEYRLMKKQIEYFRAKTPRIGMIQELDFPARSGQEVVTCDNSHYN
                     PKKPDDKDRSGFVYSVPFFDASGEMGGIVVAVMLTSAAADSLPSGNFALRNTTYNYTI
                     EHAKPGE"
BASE COUNT          839 a          728 c          894 g          699 t
ORIGIN      
        1 cttcgcacag ggaatttcaa acagcgtctt tcgtttcatg aagctggcta ccccggggcc
       61 cttcacgttt ctattcaaag ctaatcggaa catggacagg cgggccactg gaaagagaaa
      121 ggaagtgggc attcgaattg tggatcacgc tgtggtgcag gcaatcattg aagaactggg
      181 tggaccactc atgtccacaa gtgttccaga agaagagtat ttcatcgatc cggagcacct
      241 ggacgcagtc tggggaaaaa gggtgcatgc gattgtggac gggggcgtga aggaaaacac
      301 agggtcgagc atcctcgatt gtgcggacgg agaaatcaag ctggttagaa agggagcggg
      361 cgcagaaatt cttgcgcaac ttggcctgga agaataagta atccaggaat cacttcattg
      421 aacaaattcg gagccaccaa tgtcgggaca ttctagatgg gccaacatca agcacagaaa
      481 agagggagcg gacaagaaac gcggcgtagc atttgctaaa atttctaaag aattgattgt
      541 agcggctcgt ctgggcggat cagatccagc gtccaatgcc cgccttcgca tatccatctc
      601 gaaagcccgc gcggcaaata tgccgagaga caatattgaa cgtgcagtaa aaaaaggggc
      661 cggggaactg gaaggaacta catacgagga gatcctctac gaagtttacg ctccgggtgg
      721 agttggaatt ctggtggaag gtctgacaga caagaagtcc agaaccactc ctgaaatcaa
      781 aagcatcctc acaaagtatg gcggaaacct ggccatggaa ggcgccgtat ctcgcaattt
      841 tacaaggtgc ggtgtgatcg taattgaaag tcagggaaag tcagaggatg aatggatgga
      901 gtttgctctc gacgcaggag cggatgactt cgtagtccag gaaggattct ttgaaattcg
      961 cgtgccagtg gaccgttatg cggcattgca ggagaagctt tctcagaagg aagcatctgt
     1021 ggtggaatct ggaatcaaat ttgttcctct gcccgggaca gaagtagaag taaaagacgc
     1081 agatcaggcg gcaaagatcc agaagttcct ggatgccctg gaagaccacg atgacatcca
     1141 ggcagtgtct accaactggg cctgatgcgc atcctggcat ttgaccccgg atatggcatc
     1201 accggatggg gagttgtaga agagccacct cttctggccg tggactatgg agccatcgag
     1261 acaagtgcaa gagacaaaca cgaagttcgc cttctggaaa ttttgaatac ttcgcgtgaa
     1321 ctgatcggta gatttaaacc gtccttcatc gtagtagaaa ctctttttgt ttcaaagaat
     1381 gcgaagactg caggtggagt gtatgagtca cgcggagtga ttctggctgc atgtgcggaa
     1441 agcggaacag aagtcattga gcttggacct actaccatca agaaaactgt gactggaagc
     1501 gggcaggcca ccaagcaatc ggttcatcaa atggtgaaaa gactgctggg catatcgcag
     1561 aacattcgtc cggatgacac ggcggatgca ctggcagggg cgattgctgg aatactcgcg
     1621 gcaaagagca gaatggtgaa gcaaagaatt cggagcgcga aatgatttct ggaatccagg
     1681 gaaccctggt gcgcctggga gcgtcgagtg cgtttgttct ggcaggcggg ctggaatacg
     1741 aagtgtacgt gcctctgtct gtgtatgatg cactcgagaa gcagggatcc ggatctgacg
     1801 tgcatttgtt tgttcatcat cacttttcgc aggaagatca aaagctattt ggatttgcgg
     1861 atgcaagtga acgcgaagtg ttccgggaac tgattcgtct acagggaatg ggcccggccc
     1921 tggcgctctc cgttctcagc cactacgatg gtccgaggct cctttcgagt tgcgaatctg
     1981 gagatgtcca gggtttaact caaattccaa gagtgggaaa gaagactgca gaaagactga
     2041 ttttcgaaat tacgagtcgc aaagagcgct ttcgtaagct actggctggc cagagaaaag
     2101 gacccgcgcc agagacagag caagacctcg cgcgagaggc tttgttgcaa cttggataca
     2161 gagaggccgc gatcgcagaa gcgttcaaga aactcgatgc gaagcctcgc actgcctcag
     2221 aatggattac gtcttcgctt cgcgttcttt gacgttttcg catcctttgt tttctgagtg
     2281 acacacctga ctggccggtt gcaatttcgt catggccacg cttgcatcca ttcgtaagta
     2341 tctctatcca gcccgatacc agctggcagc tgtgcttgcg gccgcggtct tcctgtctgc
     2401 tctggcaatc tggaagtatc gccaggatat gcagggagcc ctgcacaatt atcgactcga
     2461 agcacacgac gaagctcttc gcgtggcgcg aaatgcgaat gacatgttcc gactcatcta
     2521 ccaggggctt cgcacaattg cacgtcttcc aggtgtgcga aagatccagg accagaacct
     2581 ggtgtttcga ggtggtgggg caggttttga agcggatgcg aagctggccg ttcaggagct
     2641 ctacaacaac ctcgccacaa acgttgccat ttcagaagta tacatcgctc ccagggatct
     2701 agacccggac gcacctgctt acagtaatgc acacaaagag cctctggtga cttttgatga
     2761 gctcatcgta ggacagactg cactcggcaa caaaggcgac aatgcagaaa gcgttcctga
     2821 gattgagctg tatgaatacc gtttgatgaa aaaacagatt gaatacttcc gggcaaagac
     2881 tccgcgcatc ggcatgatcc aggaactgga ctttcccgct cgatctggac aggaagtggt
     2941 cacatgcgac aactcccatt ataatccgaa gaagccagat gacaaggacc gatccggttt
     3001 tgtgtattca gttcctttct ttgatgcaag cggggagatg gggggaattg tggtggccgt
     3061 gatgctgaca tctgctgctg cagattcact tccatccgga aatttcgctc tgcgcaatac
     3121 tacctataac tatacaatcg aacatgcgaa acctggagag
//
LOCUS       DAOQJL010000469         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20456_length_3142_cov_4.197603, whole genome shotgun sequence.
ACCESSION   DAOQJL010000469 DAOQJL010000000
VERSION     DAOQJL010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20456_length_3142_cov_4.197603"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..643)
                     /locus_tag="PLG78_16400"
     CDS             complement(155..643)
                     /locus_tag="PLG78_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE24755.1"
                     /translation="MHPNEALLEKFYSAFQKRDAATMNSCYHEDVEFWDPVFHDLKGW
                     KARAMWSMLTERAAPDFKLTFSGIKADDNGGVAHWEPTYAFSKTGNLVHNIIDAKFEF
                     RDGKIIKHRDSFDLWRWAGQALGASGKFLGWTPMVQNKIRGEAATGLEMFIKRKKLGP
                     GK"
     gene            complement(688..1185)
                     /locus_tag="PLG78_16405"
     CDS             complement(688..1185)
                     /locus_tag="PLG78_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24756.1"
                     /translation="MKLSMTTKLIFIAIFLAAIPLSAAKPSFLADYNTTTLKVHIEDR
                     NIRVTRIEQKFDNPASAVPSSQSAAVSTGMISEAQLRSLRRLILANGFLRLKEAYGAP
                     AKERFYPYSIEVLVDGRKKKVLFRSNPSFEKQPKPFAEIEKMLTELGKEARNPEPAAT
                     DSTGK"
     gene            complement(1182..2018)
                     /locus_tag="PLG78_16410"
     CDS             complement(1182..2018)
                     /locus_tag="PLG78_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE24757.1"
                     /translation="MQSYLIIGGNSVAGQGSVQAIRNFESNAIVHATTSTAKQADDSS
                     GIAEADKTLYGIDLSKPETLDLIPAEKYKMIIFTPAFGPIGYPIASTPESDVKEALRF
                     SYHPMVELSNRLNPDLTIGFTAFYWLPHTLSAYGSMAYAKIAQEILAVENSNHFRMIR
                     AGSFVSKATRGIGLLLQRALRDTPHAAIRDLGEKWKASGKKFADFFFDYACECEKEAF
                     GAGFDRPHRQTNQSDIERGVSMVLKGDTAPIINVLGDWTWTDDKLKPLPADFRLNVRE
                     LL"
     gene            complement(2049..2945)
                     /locus_tag="PLG78_16415"
     CDS             complement(2049..2945)
                     /locus_tag="PLG78_16415"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE24758.1"
                     /translation="MKFYFSDRYNVPLPANHTFPMQKYFLLRKMLLERKIVSESELIE
                     SSPVRADDLVLAHSADYVQSILLGTVSADVMRRIGFPWSEDLVVRALAAAGGTMSAAQ
                     SALEDGFSGNLAGGTHHAHRAEGEGYCIFNDFAIAIRHLIQNGKIERAAIVDLDVHQG
                     NGNASILGGRDDIYILSIQGERNFPFRRVDSTVDVDLPDGTSDFLYLETLSKFLPAVF
                     EFKPDIVFYQAGVDPLKEDRLGRLALSLEGLIQRDRMVFEQAKSAGVPVCLAIGGGYA
                     LPVELTVTAHANTFAAARSVFG"
     gene            complement(2942..>3142)
                     /locus_tag="PLG78_16420"
     CDS             complement(2942..>3142)
                     /locus_tag="PLG78_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24759.1"
                     /translation="LANFDRGGRAVRLIGIGVSELLREGDEEQLDLFSARKRPDEKVD
                     RLIFEMQEKFGGRVKRASRSKS"
BASE COUNT          785 a          841 c          729 g          787 t
ORIGIN      
        1 gctcttcgaa atagagatcc gcatacgatc ccccgcgcga aaggccttcc gaaagaatgc
       61 gacgcgcttc ctcaacgctg atgccgaacc gtggatgaat catttgaaca agaaaaggcc
      121 ggagcgtgtg tccggcctcc atttatcgga actgttattt accaggacca agcttcttgc
      181 gtttgataaa catttccaga ccagtggccg cttctccgcg aatcttgttt tgaaccatgg
      241 gagtccagcc cagaaatttg ccagacgcac ccagagcctg accggcccat ctccacaaat
      301 caaaagaatc gcgatgcttg atgatcttgc catccctgaa ttcgaatttc gcatctatga
      361 tgttgtgaac cagattgccc gtcttagaga aggcatacgt aggttcccag tgagcgacgc
      421 caccgttatc atctgctttg attccactga aagtgagttt gaaatccgga gcggcccgct
      481 ctgtgagcat ggaccacatg gcgcgcgctt tccatccttt cagatcatga aagacaggat
      541 cccagaattc cacgtcttcg tggtagcagg aattcatcgt agctgcgtcc cttttttgaa
      601 acgcagaata gaatttttcg agaagggctt catttggatg catgaccggg cagctagcct
      661 gcccttttca gatcgggcaa gcaaaaatca ttttcctgtg gagtcagtgg cagctggctc
      721 tggatttcgc gcttctttgc caagctctgt gagcattttc tcaatctctg cgaaaggctt
      781 tggttgtttt tcaaacgatg gattgcttct aaatagaact ttctttttgc gtccatctac
      841 gagcacttct atgctatagg ggtagaacct ctccttcgca ggtgcaccat aggcttcttt
      901 caatcgcaag aaaccgttgg ccagaatcaa tcggcgaaga ctgcgcaatt gcgcttctga
      961 aatcatcccc gtagatacag cagcactctg cgaagaggga acggcactgg cagggttatc
     1021 aaacttctgt tcgatccgag ttacgcggat gttgcgatcc tcaatgtgaa ccttcagggt
     1081 ggtggtattg tagtccgcca gaaaagaagg tttggccgcg ctcaggggaa tggcagccag
     1141 aaagatcgcg atgaaaatca atttcgtggt catagaaagc ttcatagtag ttctctcaca
     1201 ttcagacgaa agtctgccgg caaaggtttt agtttatcgt ctgtccaggt ccagtctccc
     1261 agcacattga tgatgggagc tgtgtctcct ttcaaaacca tactgactcc tctttcaatg
     1321 tcagactgat ttgtctgtct gtgcggtctg tcaaagccgg caccaaacgc ttccttttcg
     1381 cattcgcatg cataatcaaa aaagaaatcg gcgaatttct ttccagacgc cttccatttc
     1441 tcacccagat ctcgaatcgc cgcgtgcggt gtatcgcgca aggctctttg caagagaagt
     1501 ccaatacccc tcgtagcctt gctcacgaaa gatccggcgc gaatcatgcg aaaatgattc
     1561 gaattttcca ctgccagaat ttcctgcgca atctttgcat aagccatgct tccatatgcg
     1621 gataaagtat gaggcagcca gtaaaaagcg gtgaacccta tagtcagatc cggattcaga
     1681 cgattagaaa gttctaccat agggtgatag gaaaatcgga gcgcttcctt tacgtccgac
     1741 tcgggtgttg aagcgatcgg ataaccaatg ggcccgaagg cgggagtaaa gatgatcatc
     1801 ttgtatttct cagctggaat caagtcgagc gtttctggtt tgctcaaatc aatgccgtat
     1861 aacgttttgt ctgcttccgc aatcccggaa gaatcgtctg cctgttttgc agtggatgtg
     1921 gttgcgtgca cgatcgcgtt tgattcgaag tttcgaattg cctgcacgct tccctgcccg
     1981 gcaacgctgt ttccaccaat gatcagataa ctttgcacgg acgaaaagtt ccttcaggaa
     2041 agcaatcgtc aaccaaatac ggaacgcgcc gccgcaaacg tattggcatg agccgtcacc
     2101 gtcagttcga ctggaagagc atacccacca ccaatcgcca gacacacggg gacacccgca
     2161 gactttgcct gctcaaaaac cattctatcg cgctggatca aaccctcgag ggaaagagcg
     2221 agcctgccaa gcctgtcttc tttcagcgga tccacaccgg cctgataaaa gacaatgtct
     2281 ggcttaaact caaacaccgc gggcaagaat ttggaaaggg tctccagata gagaaaatcg
     2341 cttgtaccgt ccggtaaatc cacgtccaca gtggaatcca cgcggcgaaa aggaaaattt
     2401 cgttctccct ggatactaag tatgtatata tcatctcgcc caccgagtat ggacgcattt
     2461 ccatttccct ggtgaacatc cagatccact atggcggctc gctcgatttt tccgttctgt
     2521 atgagatgcc tgatggcgat ggcaaagtca ttgaaaatgc agtagccttc cccttccgct
     2581 ctgtgcgcat gatgcgttcc tcccgccaaa tttccggaaa atccatcctc caatgcgctc
     2641 tgcgccgcgc tcattgtgcc accggccgcc gcaagtgcgc gcacaaccag atcttcgctc
     2701 cagggaaaac caattcgtct catcacgtcc gcgcttacgg ttccaagcag aatggattga
     2761 acgtagtccg ctgaatgagc cagaaccaga tcgtctgcgc gcacgggaga agattcgatc
     2821 aattcagact ccgatacaat ctttcgctca agaagcatct ttctcaggag aaagtatttt
     2881 tgcattggaa acgtgtgatt cgcaggcaaa ggaacgttgt acctgtcgct gaaatagaac
     2941 ttcatgattt tgaacgactg gctcgcttta cgcgaccgcc aaacttctcc tgcatttcaa
     3001 aaatcaatcg atccaccttc tcatccggcc tctttcgcgc tgaaaacaga tccagttgct
     3061 cctcatcacc ttcgcgaagt aactcggaaa ctccaattcc gatcagacgg accgctcgcc
     3121 cgcctcgatc gaagttcgca ag
//
LOCUS       DAOQJL010000470         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20502_length_3138_cov_4.361336, whole genome shotgun sequence.
ACCESSION   DAOQJL010000470 DAOQJL010000000
VERSION     DAOQJL010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20502_length_3138_cov_4.361336"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..905)
                     /locus_tag="PLG78_16425"
     CDS             complement(222..905)
                     /locus_tag="PLG78_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNE24760.1"
                     /translation="MIFLFLAIYLSAAGFSIYCRYNNRPLYNFAKGIPLVLLFIYYLF
                     HPSGSGLYYWFVIALLSGLIGDLLLLREKTFRYGALFFSLGHLFYVGAFYVMGGWPKI
                     GVLVLIFGYMAIYCAFLYKRLEETGGVGNVWLGLPYLFLVSLLLVFASATAGHVAALG
                     VPAQLEPLPLFVIDASIFVGALLFYVSDLVLVWNRNAGTFKEAQFVILSTYYLAQLFI
                     AMGAIGHLN"
     gene            complement(902..1678)
                     /locus_tag="PLG78_16430"
     CDS             complement(902..1678)
                     /locus_tag="PLG78_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE24761.1"
                     /translation="MPGFPTTTIERKNGVIGVLTLDALPPNNTFNHELLLGFTKALFD
                     LGEKCDAVLVTSANPKFFSNGLDGKFLLEADLKTREETVTAMIRAYGKMISFGKPWIV
                     EIAGHAMAGGAVISCAADYRFMLQGSGRIGFSELAVGLPLPLNYIHGIHRIVQPSAVR
                     LLIEGAAYKPEEALSIGLIDGVAEDAEKLRALVLKRFDAILRMEKEAFLPTRKIYRGD
                     LLRMIERDEEEDVRMAMQLVRSPAFERALGTIAGKNRGQS"
     gene            complement(1688..2215)
                     /locus_tag="PLG78_16435"
     CDS             complement(1688..2215)
                     /locus_tag="PLG78_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE24762.1"
                     /translation="MPSARTRIIYAVIMIALIAGEIVDVVGDFVREGFTIWLCQDVLL
                     LLLVSVGVTYILYTSAADLRGMHIHLEKARQDVASFRSRNQDAIVAMREAILSQFGAW
                     GFTPAEARIADLLIRGYSSRQIAGMLGRSERTVRNQTLSIYRKSGMTGRSDLSAFFLQ
                     DIMGEEKEFPAVQGR"
     gene            2346..>3138
                     /locus_tag="PLG78_16440"
     CDS             2346..>3138
                     /locus_tag="PLG78_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24763.1"
                     /translation="MTHRLFCLQSLAAVCFLITFALHAQTPHAEEEKKSPGDPIAEKQ
                     SVDAFSSLEDGQPSAPGEFEMVTTVSSQNSRKALNDSSVGGELRFTPQGGTFLTNMEL
                     GLGLEAGRAVVHNDQYLDRRDFMAFEAGRSLAGSSLMPADIVSFLPARNFTSRVAEPV
                     RPYANYLAGQITSSPQNFSGIVNMSAYQLLFDHETAVIDSSRLDLERHAVEHSASVNP
                     FWLERWHPESDGIPTISTIVGVSLPLTDKAASGESARLSLIVAQSL"
BASE COUNT          848 a          842 c          760 g          688 t
ORIGIN      
        1 caatgttagg atccgattga ccgagttggg aagcgtctgc tccatcgaaa agggcgactt
       61 ccggagccag cactaaaatt gacatggtat cgccaggctc cggctacacg actgaaggtt
      121 gcgattgaca acgcaacaga ccgctcagca cgaccagcga gaatatgagt ttcattcgga
      181 tccgtccgaa tgcaaagtca gttcgtaact tcacttcaca atcaattcaa atgcccgatg
      241 gcacccatgg caatgaagag ttgcgcaaga tagtatgtgg acaaaatcac aaactgcgct
      301 tccttgaagg ttccagcgtt tcgattccag acaagaacca gatcggatac gtagaacaaa
      361 agtgctccga caaaaatgga agcatcaatg acgaataacg gtagaggttc cagttgagct
      421 ggaacgccca atgctgctac atggccggca gtggcgcttg cgaagaccag aagaagactc
      481 acgaggaaca gatatggcag accaagccac acatttccca ctccacccgt ctcctccagg
      541 cgcttgtaaa gaaaagcaca gtaaatggcc atgtatccaa agatcaaaac cagaacgccg
      601 attttgggcc atccacccat cacgtagaag gcgcccacat agaacaaatg gcccagagag
      661 aaaaacaaag ccccatagcg aaatgtcttt tcgcgcaata gcaacagatc cccgatcagg
      721 ccggacaaca aagcaatcac aaaccagtag taaagaccgg aaccggacgg atgaaacaaa
      781 tagtaaatga aaagcaaaac gagcgggatg ccttttgcga agttgtagag cggccggttg
      841 ttgtagcgac agtagatgga aaagccagcc gcagaaagat agatggccag aaaaagaaag
      901 atcatgattg gcctctgttc tttcctgcga ttgtgccgag agcccgttca aatgcgggag
      961 agcgaaccag ttgcatggcc atgcgaacat cttcctcttc atcgcgctcg atcattctaa
     1021 ggaggtctcc tctatatatt tttctcgtgg gaagaaacgc ttccttctcc attcggagga
     1081 tggcatcaaa tcgcttcagc acaagagcgc ggagcttttc tgcgtcttct gccacaccat
     1141 cgatcaaacc aatagacaga gcctcctctg gcttgtatgc ggcaccttcg atcagcaatc
     1201 gcaccgcaga tggttgaacg attctatgga ttccgtgaat atagttaagc ggaagaggta
     1261 gacccaccgc aagttcagag aatccaattc gtccggatcc ctgcagcata aaacgataat
     1321 cagcggcaca cgaaattaca gcgccacctg ccatcgcatg ccctgcgatc tccacaatcc
     1381 agggcttccc gaatgaaatc atctttccgt aagcgcgaat catggcagtc accgtttctt
     1441 cgcgagtctt caagtccgct tcgagcaaaa actttccgtc caggccattt gaaaaaaact
     1501 tagggttggc agacgttacg agaaccgcat cgcacttttc gcccaggtca aagagcgcct
     1561 tcgtgaatcc aaggagcagt tcatgattga acgtgttgtt tggcggcaac gcatccaatg
     1621 tcaaaacgcc aatgacgccg ttctttcttt ctatggtcgt ggttgggaag ccgggcatgg
     1681 gccagactca tctaccctgg actgcgggaa attctttttc ttcgcccata atgtcctgca
     1741 gaaagaaggc agacagatca ctcctacctg tcatgccaga ctttctataa atcgaaaggg
     1801 tctgattgcg cacagttctt tcgctgcgac cgagcattcc tgcgatctgc ctgcttgagt
     1861 atccacgaat caacaaatca gcaatgcgag cttctgcagg cgtaaaaccc caggcgccga
     1921 actgactcag gatggcttct ctcatggcaa cgattgcgtc ctggttgcgc gatcgaaacg
     1981 acgccacatc ctgcctggcc ttctcaagat gaatgtgcat cccgcgaaga tccgcggcgg
     2041 aagtatacag gatataagtt acccctacgg aaaccagaag aagcaaaagc acatcctggc
     2101 agagccagat ggtaaacccc tctcgcacga aatcgcccac tacatctacg atttcgcctg
     2161 caatcaaagc gatcatgatc acagcataga tgatccttgt tcgggcagaa ggcatcgctc
     2221 aaattccttt tgttgccgtg cgtgtaaatc ctttccaatc caggggtgag tcaaatggct
     2281 cacgatggcg attcgggtca tttgactcat tgcgtcctat cccaggaaat gctatgatac
     2341 gaactatgac ccatcgactt ttttgcctgc agtctctggc cgccgtttgc tttttgatca
     2401 ccttcgccct gcacgcgcag accccgcacg cagaggagga aaagaaatca ccgggagatc
     2461 caatcgcaga aaagcaatcc gtggatgcat tctcaagcct ggaagatggc caaccgagcg
     2521 cacctggaga atttgaaatg gtgacgactg tctcatcgca aaactcgagg aaggctctga
     2581 acgactcctc agtcggaggt gaactgcggt tcactccaca gggaggaacg tttcttacca
     2641 atatggaact gggtctcggc ctcgaagcag gacgcgcggt cgtacacaat gaccagtatc
     2701 tggatcggcg tgacttcatg gcctttgaag caggccgatc tctggcgggc agctctctta
     2761 tgccagcaga catagtttcg tttctgcccg cgcgaaattt tacctcaaga gtagcagagc
     2821 ctgttcgtcc gtatgcaaac tatctcgcag gtcaaatcac gtcttcgcct cagaactttt
     2881 ctggaatcgt caatatgagc gcctatcagt tgttattcga tcatgagacc gcagtgatcg
     2941 attccagccg tctggacctg gaacgccacg cagtcgaaca cagtgcgagc gtgaacccat
     3001 tctggctgga aagatggcat cctgaatcgg acgggattcc taccattagc acgattgtgg
     3061 gcgtatctct tccgctcaca gataaagctg ccagtggaga gtcggctcgc ctttctctca
     3121 tagtggcgca gtctctcg
//
LOCUS       DAOQJL010000471         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20545_length_3134_cov_3.870412, whole genome shotgun sequence.
ACCESSION   DAOQJL010000471 DAOQJL010000000
VERSION     DAOQJL010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20545_length_3134_cov_3.870412"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PLG78_16445"
     CDS             <1..805
                     /locus_tag="PLG78_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE24764.1"
                     /translation="AKSLSVKEEARIMMPAAGAGLAATAVLFVFLFGASKISGKPGAS
                     GDLLPPDQTADFLKDFRALSKVDISTAGLDGYEGDENAYIIIQKFADFMCPHCYHTSK
                     ELQKAQARWPGRIRIYYRHFPLDGACNPVVQRKDPNYGPMRCNGAKAALCGGEQKIFP
                     ALYHGIFELQNEPEGISIPNLQAVTEKSGGNWVKLNACMNSPKTMAALLRDVKDAEAI
                     KLNSTPTLIVQDRLLPPGTPDEKYFMSLMDALVFEKEGAKGYESYGRSK"
     gene            802..1278
                     /gene="ribH"
                     /locus_tag="PLG78_16450"
     CDS             802..1278
                     /gene="ribH"
                     /locus_tag="PLG78_16450"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNE24765.1"
                     /translation="MSQGGFASGPLDGKGQRHAVLVARFNSDITEALKEGAIAAFARA
                     GVKAEDVEIFYVPGAFEFGSAAKAIALTGRYSAVTCLGCVIQGDTDHHLYVSGEAARL
                     IGQAAYDTGIPVIFGVLTTHTHKQALERAGATGNKGQEAADTAIEMALLLKRIRGS"
     gene            1275..1718
                     /gene="nusB"
                     /locus_tag="PLG78_16455"
     CDS             1275..1718
                     /gene="nusB"
                     /locus_tag="PLG78_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNE24766.1"
                     /translation="MKGRHKNSHAREVALQGLYQAEIGSTPVAEILRFKWLNESLEPE
                     EKTLASELISGVLDHAAALDQVLSAFSNIDFTQISSIVRCIIKMGIYEMRVTGRDPAI
                     VMDDLLNLARRYDGEQAVPFVNGVLDRYRKEMQEAAVKRMTQDES"
     gene            1715..>3134
                     /locus_tag="PLG78_16460"
     CDS             1715..>3134
                     /locus_tag="PLG78_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24767.1"
                     /translation="MTRFTLDEPQSSSPLSGDPLKARRRFRLYVLMGVLGLVLFPALV
                     WIGYYFYKKQTQAPQTALDEKIQRVDLEGLKDKIYLPDEQVSPPLQKAISLYKENFKD
                     QAKREFNNFLNTGASDHEKAIALTYLAVMDMESEKYPVAEHNLLRALNKDEKFVPALV
                     NLAITEQKLGKNGEARQYAEKARELAPGDPRVAALLGNLMAAGQDLSGAIDAYRQGIK
                     QNPDDATLTYNLAVSLLRKQNYEEAILYFSKVMQIAPGTEIAAKSSAQLGQIYFSKGN
                     FEMAADHMQKAVQSAPENAKYLYNLGIIYLRMKRNAEAASAFQKALESGSGDAQVFRA
                     LSSAFVELGQPGMAIKAIKKGLYLNPDDLTSLFALGDLYLGEKDLLNAADTYKRIVNL
                     TPGDRNTEDALVKLGTVYTDLERFNDAQDALQKAVSINPSNVQASYALGIVYRKAGRM
                     ELAIQAWKRALNPPGLSGDRTGL"
BASE COUNT          838 a          792 c          819 g          685 t
ORIGIN      
        1 cgcaaaatct ctttctgtga aggaagaagc ccgcatcatg atgcctgcag caggtgccgg
       61 ccttgcggct accgctgtcc tgtttgtttt tctgtttggt gcgtccaaaa tttcaggtaa
      121 gcccggtgca tccggagatc ttcttcctcc ggatcagacc gcagatttcc tcaaagactt
      181 tcgcgcactc agcaaagttg atatctcgac tgctggcctc gacggatatg agggagatga
      241 aaacgcatac atcatcattc agaagttcgc agatttcatg tgccctcatt gctaccacac
      301 aagcaaggaa ctgcaaaagg cacaggctcg ctggccaggc agaattcgca tttactatcg
      361 tcactttcct ctggatggag cgtgcaatcc tgtagtgcag cgcaaggatc ctaactacgg
      421 acccatgaga tgcaacggag caaaagcggc actctgcggc ggtgagcaga aaattttccc
      481 tgcactctat cacggaattt ttgaacttca gaatgaaccg gaaggaatct ctattccaaa
      541 tcttcaggca gtcacagaga agagcggtgg caactgggtg aagctcaatg catgcatgaa
      601 ttctccaaaa acaatggctg cactccttcg cgacgtgaaa gatgcagagg caatcaaact
      661 gaattcgact ccaactctca tcgttcagga cagactcctc ccaccaggca ctccggatga
      721 aaaatatttc atgagtctca tggatgctct cgtctttgaa aaagaaggcg cgaaaggtta
      781 cgagtcttac ggcagatcga aatgagtcag ggcggctttg cttcgggtcc tctggatgga
      841 aaaggacaga ggcatgccgt tcttgtggcg cgtttcaatt ctgatatcac agaagcactc
      901 aaagaagggg cgatcgctgc tttcgctcgc gcaggagtca aagcggagga cgtagaaatt
      961 ttctacgttc caggtgcctt tgaattcggg agcgctgcaa aggcaatcgc gctgactgga
     1021 cgttattcgg ccgtgacctg tcttggatgt gtcatccagg gagacacgga ccatcatctc
     1081 tatgtttcag gagaggcagc ccggctcatt ggccaggcag catacgacac aggcatcccg
     1141 gtcatctttg gagttctgac cactcacact cacaagcagg ccctggaaag agcgggggcc
     1201 accgggaaca aaggtcagga agcggcagac acagccatag agatggcgct cctcctgaag
     1261 cggattcgcg gctcatgaag ggtcgccata agaattccca cgccagggaa gtcgccctgc
     1321 aaggcctcta tcaggcagag atcgggagca ctcctgtagc tgagattctg cgcttcaaat
     1381 ggctcaatga gtccctggaa ccagaggaaa agaccctggc aagcgagctg atcagcggag
     1441 ttctggacca tgcggccgct ctagaccagg ttctgtccgc tttctccaac attgatttca
     1501 cgcagatttc ctcaatcgtc cgatgtataa taaaaatggg catttatgag atgcgcgtca
     1561 ccgggcgtga cccggcgata gtaatggacg atcttctgaa tcttgcgcgt cgttatgacg
     1621 gagagcaggc cgtacccttt gtaaacgggg tgctggaccg gtatcgcaaa gaaatgcagg
     1681 aggccgccgt gaagcgcatg acccaggacg aatcatgacg cgctttactt tagacgaacc
     1741 acaatcctct tctccgctat ccggcgatcc tctcaaggca cggcgccgat tcagactcta
     1801 cgttctcatg ggtgtgcttg gactcgtgct tttcccggca ctggtctgga ttggatatta
     1861 cttctataag aagcaaaccc aggcaccaca gacagctcta gacgaaaaga tccagcgtgt
     1921 ggatctggag ggtttaaaag acaaaatcta tctgccagat gagcaggttt ctcctccact
     1981 tcaaaaagca atttcactgt acaaggaaaa cttcaaagat caggcaaaac gcgaattcaa
     2041 caattttcta aacaccggcg ccagtgatca tgaaaaggcg atcgcactga cgtatctggc
     2101 cgtgatggat atggaatcag aaaagtatcc cgtggcagaa cacaacctgc ttcgcgctct
     2161 aaacaaagac gaaaagtttg ttccggccct ggtcaacctt gccatcacag aacagaagct
     2221 tggaaaaaac ggagaggcca ggcagtacgc agagaaagca cgtgaactgg ctccagggga
     2281 tccgcgtgtg gcagctctac tcgggaatct gatggcggca ggccaggatc taagcggtgc
     2341 cattgacgcc tatcgtcagg ggatcaaaca gaatccggac gacgcaactc taacatacaa
     2401 cctggctgtt tctcttctga gaaagcagaa ctacgaagaa gcgatcctat atttttctaa
     2461 agtaatgcag attgcacctg gaactgagat cgccgcaaaa tcgtccgcgc aactggggca
     2521 gatttatttc agtaagggca acttcgaaat ggccgcggat cacatgcaga aggcagtgca
     2581 gtctgcgcca gaaaacgcaa agtatctcta caacctggga atcatctatc ttcgcatgaa
     2641 gcgcaatgcg gaagcggcca gcgcatttca aaaagctctg gaatctggtt caggagacgc
     2701 gcaggtcttt cgcgctctca gttctgcgtt tgtggagctg ggacagccag gcatggccat
     2761 caaagcaatc aagaagggtc tctatctcaa tccggatgat ctgacgagtc tatttgctct
     2821 gggcgatttg tatctaggag aaaaagatct gctcaatgcg gcggacacgt acaagagaat
     2881 tgtgaatctc actccaggag atcgcaacac agaagatgca ctcgtgaagc ttggcacagt
     2941 atatacagat ctagaaagat tcaacgatgc gcaggacgca ctgcagaaag ctgtttccat
     3001 caatccttcg aatgttcagg caagctacgc ccttggaatt gtgtatcgca aagcgggaag
     3061 aatggagctt gcgattcagg cctggaaaag agcgctcaat ccacctggat tgtcggggga
     3121 tcgcaccggg cttt
//
LOCUS       DAOQJL010000472         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20597_length_3129_cov_3.576448, whole genome shotgun sequence.
ACCESSION   DAOQJL010000472 DAOQJL010000000
VERSION     DAOQJL010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20597_length_3129_cov_3.576448"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="PLG78_16465"
     CDS             complement(<1..2321)
                     /locus_tag="PLG78_16465"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24768.1"
                     /translation="MKASCVTLGALLTLLPSMLDAQALDLTNICAKPEDTSCVSWQYR
                     RGFDTDVFGKNPGELTDGWKKIEAFPVWMSKMEPPESTFETYTFVAYFHAPATLLEQK
                     QPGIAFQEIGEAFEIYINGNLVGSEGLVQNNRIITHRTVRDAIYEFDRSFLQPGVNQL
                     VVKIHGDPRYDHTGFYAVRGYKIDSYGTLVNSREDRIGLVLISLYLFVGVYHLLLFWK
                     RRQDRYNLFYALFAILLFVYLMTRHYTIFELPYDTRMIQRVELVVLYPLGPWLMLFFD
                     YLFLNRARRLTWIFAAFNGLLSLITLFLPMHLAEVVLCIWQATVPILFVYGIFILAAA
                     MRAKNRDAWRLLLGMVLFIVGAAFDILDSAKFHTGLTLTKYSFSIFVIGIAVILANRF
                     MRVHNSVEELNETLEKKVEDRTRELSETLSTVQAMKTQQDGDYFLTSLLISPLNANRA
                     DSRIFGVETLISQKKKFKFKKWEAEIGGDIVTAHTIELAGRRYVAFLNGDAMGKSIQG
                     AGGALVLGTVFKSLITRTQTSTLLQSRMPERWLKECFLELQNTFVSFDGTMLISAVVG
                     LADEATGFIYYINAEHPWVVGYYQGQARFIEDEMALRKIGVYGLEGSLSVKTYQMKPG
                     EVLILGSDGRDDLAMNVDSEGKRLINEDSDLFLRLVEKGNGDLHNIFESIEKTGEIID
                     DVTLMRIGYLEDGPFDRVEPSAECSENLRLARQSQASDRATDAILYLNRAVVDPTCPP
                     SAHLDLAQTHMRLKNYAEAFSSARAFTALDPQHV"
     gene            complement(2330..>3129)
                     /locus_tag="PLG78_16470"
     CDS             complement(2330..>3129)
                     /locus_tag="PLG78_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE24769.1"
                     /translation="PLTSGEEEVRHVVIDIRESDLRYLEGQSLGVLAPGVQENGKPHK
                     LRLYSIASPRGGDADHPGTVSLCVKRLIEKKEDGSIYQGIASNYICDLKPGDDVLVTG
                     PVGMHFLLPADDRTNIIMVATGTGIAPFKSFLDHIFRERSTPWRGTLALFFGAKFKNE
                     LIYCNEMDASLRQFESRPHFQHVCAISREEMNAEGGRMYVQHRIDENYDSIRSLLAED
                     NFALYICGLKGMEKGIEDVFRARLGEEEWEHKKQLYRKDGRWIVEVY"
BASE COUNT          773 a          778 c          759 g          819 t
ORIGIN      
        1 acgtgttgcg gatccagcgc cgtgaaagct cgtgcgctgc tgaatgcttc cgcataattc
       61 ttaagacgca tatgagtttg tgccagatcc agatgtgcag aggggggaca ggtaggatct
      121 acgactgcgc gatttagata gaggatcgca tccgtagccc gatctgatgc ctgtgattgt
      181 cgcgcaaggc gaaggttctc gctgcattcg gcagacggct caactctatc aaatggtccg
      241 tcttccaggt aaccgattcg catgagagtc acgtcgtcga tgatttcacc tgttttttca
      301 atggattcga agatattatg aagatctcca tttccttttt ctacaagacg aaggaataag
      361 tcgctgtcct cgttgatgag acgtttgcct tccgagtcga cattcatagc caggtcgtcg
      421 cgaccatctg agccaaggat cagcacttct cctggtttca tctgatatgt ttttacggag
      481 agacttccct cgagcccgta gactccgatt ttgcgaagag ccatttcgtc ttcaataaac
      541 ctcgcctggc cctggtagta gcccacaacc cacgggtgtt ccgcgtttat gtaataaatg
      601 aatccagttg cttcgtctgc cagaccaacc acggctgaaa tcagcatggt tccatcgaag
      661 gacacgaatg tattctgtag ttccagaaag cattctttca gccatcgttc tggcattctg
      721 ctttgaagca atgtagacgt ttgtgtgcgc gtgatcaagg atttgaatac agtgccgagc
      781 acaagagctc cacctgctcc ttgaatcgat ttccccatgg cgtctccgtt gaggaaagcc
      841 acataacgtc ggccggcgag ttcaatggta tgtgcagtta caatatcgcc gccaatctct
      901 gcttcccatt tcttaaattt gaacttcttc ttctggctga tgagagtttc cactccgaag
      961 attctgctat ctgcacggtt tgcgttgagc ggcgaaatca gtagagaagt gaggaagtag
     1021 tctccatcct gctgtgtttt catggcctgg accgtgctca gagtttcgct cagttcgcga
     1081 gttctatctt ctaccttctt ttcaagtgtt tcattcaatt cttccactga attgtggaca
     1141 cgcatgaatc tgttcgcgag aatcacagca attccaatta caaaaatgga gaatgaatac
     1201 ttggtgagag tgagcccggt gtgaaatttt gcagagtcga ggatatcgaa cgcagcgcca
     1261 acgatgaaca ggaccatccc cagaagcaaa cgccaggcat cgcgattctt tgctcgcatg
     1321 gctgccgcga gaatgaaaat gccgtacacg aacaaaatcg gcacggttgc ttgccagata
     1381 caaagaacaa cttccgccag atgcatgggg agaaacaatg tgatcaggga caatagaccg
     1441 ttgaaggctg cgaagatcca tgtgagcctg cgagctctgt tcaggaagag gtagtcaaag
     1501 aacagcatca gccagggacc caatgggtac agtacaacca gctccactct ctggatcatt
     1561 ctcgtgtcgt agggtaactc aaaaattgtg tagtggcgag tcatcagata cacaaagagt
     1621 agaatggcaa acaacgcata aaacaggttg tatctgtcct gacgccgttt ccagaataac
     1681 aatagatgat aaacaccaac aaacaggtag agagaaatca gtaccaggcc aatgcggtct
     1741 tcgcgtgagt ttaccagcgt tccgtatgag tcaattttgt accctctaac tgcatagaac
     1801 ccggtatgat cataccgcgg gtcaccatgg attttgacaa ccagctgatt gactccaggt
     1861 tgaagaaagg atcgatcgaa ttcatagatc gcatcccgca cagttctgtg cgtaatgatt
     1921 ctgttgttct gtaccaaccc ttcagagccg accagatttc cgttgatata gatttcgaat
     1981 gcttctccta tttcctggaa agctatgccc ggctgttttt gttcgaggag tgtggccggc
     2041 gcatgaaagt atgctacgaa cgtatacgtt tcaaatgtgg attccggagg ttccattttg
     2101 gacatccaga cggggaaggc ttcaatcttc ttccagccgt ccgtgagctc gcccgggttt
     2161 ttcccgaata catcggtgtc aaagccgcgg cggtattgcc aggatacgca ggaagtgtct
     2221 tccggtttcg cgcaaatatt tgtcaggtca agggcctgcg catcgagcat cgacgggagt
     2281 agcgtaagaa gagcgcctaa cgtgacgcaa gaggctttca tccaaccagc tagtaaacct
     2341 ctacgatcca tcgcccgtct tttcggtaca actgcttctt gtgttcccac tcttcttccc
     2401 ccaggcgcgc acggaaaaca tcttcgatgc ctttctccat gccctttaag ccgcaaatgt
     2461 aaagtgcaaa gttgtcttca gcaagcaatg accgaattga atcgtagttt tcgtctattc
     2521 tatgctgaac atacattcta ccgccttccg cattcatttc ttctcgcgaa atcgcacaca
     2581 cgtgctggaa atgcgggcgg ctttcgaatt gccgtaagct agcgtccatt tcattgcaat
     2641 agatgagttc gttcttgaat ttagcaccaa agaacaatgc taaagtcccg cgccacggcg
     2701 ttgaccgttc gcggaagata tgatcgagaa atgatttgaa aggtgcgatt cccgtgccag
     2761 tggcaaccat aatgatattt gttcgatcgt ctgccggaag caggaaatgc attccaacgg
     2821 gcccggtgac aagcacatca tctccaggct ttagatcgca gatgtagtta gatgcgatgc
     2881 cctgatagat agatccgtct tctttctttt ctatgagtcg tttgacgcaa agactgactg
     2941 tgccgggatg atctgcgtcc ccaccgcgag gagatgcgat gctatagagg cgtagcttgt
     3001 gtggttttcc attctcctgg acgcccggtg ccagaacccc cagggattgc ccctcgaggt
     3061 atcgcagatc actttcgcgt atatcaatga caacatgccg aacttcttct tcgccgctag
     3121 taagtgggc
//
LOCUS       DAOQJL010000473         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20628_length_3126_cov_4.358841, whole genome shotgun sequence.
ACCESSION   DAOQJL010000473 DAOQJL010000000
VERSION     DAOQJL010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20628_length_3126_cov_4.358841"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PLG78_16475"
     CDS             <1..1339
                     /locus_tag="PLG78_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE24770.1"
                     /translation="ASAGKEGPAAQIGAGIGSLVANRIQIGPRARRTMLLAGAAGGLG
                     AVFHAPLAGALTAVEMLYREDVESDSLVPCIISSVSAYLVYTMIRGTGPIYPSTDLSF
                     RALDLPFFAILGLLCFVCGFVFVRTYEFISHSFTRSRIPLLLQPILGAILVFAVAAGF
                     PQVLGSGAETLRGLLAQPSFPATFTLCAWFLMLGFAKMIASSLTVGSGNSGGLFGPSL
                     LMGAYLGLAVGTAAHLFLPGQTASLHSYALVGMGAFYSGIASAPIAGMLMVCEMTGSY
                     RLLPGLILVTTLALVLSQKWSIYKSQLHNRFQSPAHDWDIKMDFLERARLGESKIEIR
                     RHAMVKVDATLATLRKLAARVHASDFVVFDKEHYSGMISLKQYQGRQNSRIRPDLTVP
                     AVSPRDTLARALRIMSERELDKVAVVDEGRILGYVRQRDVVGFYFSSVRGGTS"
     gene            complement(1320..2609)
                     /locus_tag="PLG78_16480"
     CDS             complement(1320..2609)
                     /locus_tag="PLG78_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE24771.1"
                     /translation="MKRVCVIGAGPSGLAAARNAIQAGLGDGLVVFEKSGEVGGNWVF
                     REGAGHSSVYESTHIISSKYHSAYEDFPFPEGTADYPSHKELRRYFESYATHFGVHGK
                     IRFRTEVLSAIPEGDQWRITSKGPRGKKTETFDALMVANGHHWDPSMPALPGKFSGRF
                     MHSHDFKHARPFAGKRVLVMGGGNSAADVAVECSRVAAHTTISMRHGHWFLPKFVFGV
                     PNDVLYHRSLWLPGFIRQPLLRWTILLLQGSNSRYGLPEPSYDILEGHPTLNSELYYF
                     IRHGEISPKPGIKSVKGSRVTFADDSSADFDVIIAATGYNIVFPFLDKSIVDLSGKTE
                     VGLWRKMFHPHYHNLYFIGLFQPLGCIWPLADFQAKLACQEILGKYERPSNIGKAIDK
                     EIKKPHYKFVRSPRHSTEVDYPRFRRELLTEIRKCRP"
     gene            complement(2680..>3126)
                     /locus_tag="PLG78_16485"
     CDS             complement(2680..>3126)
                     /locus_tag="PLG78_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24772.1"
                     /translation="LVATVSENAYNGPYAETGLSFYTVRARSPAGESLDSPSEPVFAD
                     EIRPAPRGRGTEAGIERFTGKWKGTFMASGAMPTNVTAEIRNSGGMLSGSFQIEKGSA
                     ISFSAPYAEGASAATSQTAELRLMPFGLLELHIHSPGVEEVVVLER"
BASE COUNT          719 a          812 c          819 g          776 t
ORIGIN      
        1 tgctagcgca ggtaaagaag gaccggcggc tcaaattggc gcaggcattg gatcgctcgt
       61 tgcaaatcga attcaaatag ggccgcgggc tcgccgaacg atgttgttag ctggagctgc
      121 gggaggtctg ggagctgtgt ttcacgctcc gctcgccgga gcactcactg cagttgaaat
      181 gctctaccgc gaagacgtcg aatccgattc actggttccg tgcattattt cttccgtaag
      241 cgcctatctg gtctacacga tgattcgagg cactggcccc atataccctt cgacagatct
      301 gagctttcgc gccctcgatt tgcccttctt tgcgatactg ggactgctgt gttttgtttg
      361 tggatttgtt tttgttcgca cgtatgaatt catttcgcat tcgtttacga ggtctcgcat
      421 tccgctgctc ctccaaccga tcctgggcgc cattcttgtt ttcgcggttg ctgcgggctt
      481 tcctcaagtg cttggcagcg gagcagaaac gttgcgcgga ctcctggccc agccgtcatt
      541 tcccgcaacg ttcactttgt gcgcgtggtt tctaatgctt ggatttgcga agatgatcgc
      601 aagcagtctc actgttggat cgggcaactc gggtggatta tttgggcctt ctcttctcat
      661 gggcgcctat cttggccttg cggttggaac tgccgcgcat ttgtttcttc ctgggcaaac
      721 cgcaagcctc cattcgtatg ctctggtggg gatgggcgca ttctactctg gaattgcaag
      781 tgcacccatt gcggggatgt taatggtatg cgaaatgaca ggcagctacc gactcctccc
      841 tggattgatt cttgtcacca cgctcgcact cgttctttcg cagaagtgga gcatttacaa
      901 aagccagctc cacaatcgct ttcaatcccc tgctcatgac tgggatatca agatggattt
      961 cctggaacgg gcgcgacttg gcgaaagcaa gattgaaatt cggcggcacg cgatggtaaa
     1021 agttgacgcg actctggcca ctcttcgcaa gcttgccgct cgagtgcacg cgtccgattt
     1081 cgttgttttc gacaaagagc actattcagg aatgatttcg cttaaacaat atcagggcag
     1141 acagaactca agaatccgac cagatcttac agtgcccgct gtttctccac gcgatactct
     1201 ggcaagagca ctccgcatca tgtctgaacg cgagctcgac aaagtagcgg tggtagatga
     1261 aggaagaatt ctcggctatg tgcgacagag agatgtagtc gggttctact tcagctctgt
     1321 caggggcggc acttcctgat ttcagtcagg agttctctgc gaaatctggg atagtccacc
     1381 tccgttgaat gacgcgggga ccggacaaat ttatagtgag gcttcttgat ttctttatca
     1441 atcgccttac caatgttaga tggtctttcg tatttaccaa ggatttcctg acacgcgagc
     1501 tttgcctgaa aatctgcgag aggccagata caccccaatg gctggaacaa tccaatgaaa
     1561 tacaggttat gatagtgtgg atgaaacatt ttgcgccata gccccacttc cgtttttccg
     1621 gaaaggtcca cgatcgattt gtcaagaaat ggaaacacaa tgttgtagcc ggtggccgca
     1681 atgattacat caaaatcagc acttgaatca tctgcgaatg taacacgcga acctttgacg
     1741 ctcttgattc ctggctttgg agatatttca ccgtgacgaa tgaagtagta gagttcggaa
     1801 ttgagagtag gatgtccttc tagaatgtcg tatgacggtt caggaaggcc gtatcgcgaa
     1861 ttggagccct gtagaagcaa gatggtccag cggagtaaag gctgacgaat gaatccggga
     1921 agccacagac tccggtggta gaggacgtca ttgggcactc cgaagacgaa cttgggcaaa
     1981 aaccagtgac catgacgcat agatatagtt gtatgtgcgg ctacccgcga gcactccaca
     2041 gccacgtccg ctgctgaatt cccacctccc attacgagca ctcgcttgcc cgcaaaagga
     2101 cgcgcgtgtt tgaaatcgtg agaatgcata aaacgaccgg agaattttcc gggcagagca
     2161 ggcatggacg gatcccagtg atggccgttg gccaccatga gggcatcgaa tgtttctgtt
     2221 ttctttccgc gcggaccttt tgacgttatg cgccattgat ctccctccgg gatcgctgaa
     2281 agtacttccg tgcgaaagcg tatcttgcca tgaacaccaa agtgagtggc ataggattca
     2341 aagtaacgtc tgagctcttt gtgtgaagga tagtctgcgg tcccctccgg aaacggaaaa
     2401 tcttcgtacg cggaatggta ctttgaacta atgatgtggg tgctttcgta aacgctcgaa
     2461 tgcccggctc cttcgcgaaa tacccagtta ccgcctacct caccgctctt ctcgaagact
     2521 accaggccat ctcccaggcc tgcctgaatt gcattcctgg ccgccgcaag accggaagga
     2581 ccggcaccaa ttacacaaac gcgtttcata ggccagctct agcaagagcc gggtgtatgt
     2641 caattctgcc gcgacaacag aagtggacgc ggcaggcaat caccgttcga gtacaacgac
     2701 ctcttcgaca cccggagaat gaatgtgtaa ttctagaagt ccgaaaggca tcaggcgcag
     2761 ctctgctgtc tgagatgtag ccgcgctggc gccctctgcg tacggagcag aaaatgaaat
     2821 agcgcttcct ttttcgattt gaaacgaacc cgatagcatg cccccggaat tgcgaatctc
     2881 cgcagtgacg ttagtcggca ttgctcccga ggccatgaac gtgcccttcc attttccagt
     2941 aaatcgctca ataccagctt ccgtgcccct accgcgaggc gcagggcgaa tctcatccgc
     3001 aaacacaggc tcagatggag aatccagcga ttctcctgca ggagacctgg ctcgcacggt
     3061 atagaaggat agcccggttt ctgcataggg accattgtat gcgttttctg aaacggttgc
     3121 gacaag
//
LOCUS       DAOQJL010000474         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20654_length_3124_cov_4.181167, whole genome shotgun sequence.
ACCESSION   DAOQJL010000474 DAOQJL010000000
VERSION     DAOQJL010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20654_length_3124_cov_4.181167"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PLG78_16490"
     CDS             <1..1171
                     /locus_tag="PLG78_16490"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNE24773.1"
                     /translation="EVRGSFDPTLFDGRAVPESLLENLPEQKRKEMEQKYRDWHRDAW
                     KNMSKEERAEYKKKFEVQYATLYFAQDLGVRFPKSDDQPGGDLVAPKFPDGTVPKIRE
                     GEDRRVVFATWLTAQPRFRKVLINRIWTRLMGWSFFAPLDDWNENTKIQNEAVLGHLD
                     KVFVEKNMRIKDLIYYIVTSKAYARRTSAPGDSDPSSIAYFQATRLDPDQLMNSILRA
                     SGSLDVKAGDLRERSIAGADRFSGRGKLLKTEENAKEIQNAYQVPRPADDRSILAVFG
                     SGDRMDIADDDKSITVEQVLTLLNGRFTGRLSWDYGKAGSAVAATYDRTKDMNQVYDT
                     VFLSVLSRPAKAEEKERLRVLSAEKVGREKGEYRPEFLQDVVWAMFNSQEFLHVR"
     gene            1161..2495
                     /locus_tag="PLG78_16495"
     CDS             1161..2495
                     /locus_tag="PLG78_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNE24774.1"
                     /translation="MCADRFYPRGPAIVSGKREGMQSRKEFLQKSAALLGAFFLPGHL
                     MPEEDYEDITVPSSVKSVIFLNMAGGMSHVDTFDPKAGQSVFPGVATSISGIQFAETM
                     KATAKEASRVSLIRSTWSEDGDHSFGQKLLHTGYRVPQAQAFPDIPSFGAIIAYAKKR
                     KDKGPYFPSHITMGSRGGLTGRSGFLGVKYGAFQIGNLDNPVTHLKPAVGRIAEDRKG
                     RRGKMLDMLNESFSKDTSSAQVDQWKQMYLAALDFANSERLDVFDISKEKPELRAKFG
                     ESSIGKSLLMAKRLAQAEVPFIELTVGGWDTHNDNAAKIKGITKDLDPALAALLGELG
                     SSGLLKQTLVVLSSEFGRTPDVGTRDGRDHWPKVWTTLIAGGSIPQGAVIGESDPKGQ
                     KPAKRPVHVREIVATIYRASGVDPDAHPYNSMGRPFPLVPRGTEAVSELVGN"
     gene            2504..>3124
                     /locus_tag="PLG78_16500"
     CDS             2504..>3124
                     /locus_tag="PLG78_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HNE24775.1"
                     /translation="MKFWTRAGLILGLLCAIGVLANETANRKRHPLDLVYEKFAGTSI
                     KTADPDTVLRRMSLHLRGTIPGTKEVLAYEKADPETRDLSFAVDLLRSPDFAGYWGSY
                     FDSLFRGQTTAWKVPQAAFFKYIAESLHANKPYDVLVREMITSSGSPDKNPAVYFYVR
                     DGADPLQVAEYVGRVFDARRVGCARCHDHPFDTGFTRRDYYALGAFF"
BASE COUNT          827 a          725 c          834 g          738 t
ORIGIN      
        1 cgaagtgcgc ggttcttttg atcccactct gtttgatggt cgcgcagttc cagaatctct
       61 cctggaaaac ttgccggaac aaaaaagaaa agaaatggaa cagaagtatc gagactggca
      121 ccgcgatgcc tggaagaaca tgtccaaaga ggagcgagcg gaatacaaaa agaaatttga
      181 agttcagtat gctactctct acttcgcgca ggatcttgga gtgcgatttc caaaatccga
      241 tgaccagcca ggaggggatc tggtggcgcc caaatttcca gatggaacgg ttcctaagat
      301 tcgcgaagga gaagataggc gagttgtatt tgccacatgg cttactgcac agcctcgttt
      361 tcgcaaagtt ctgatcaatc gtatctggac caggctcatg ggatggagct tctttgcgcc
      421 cctggacgat tggaatgaaa acacgaagat tcaaaatgaa gcagtgctgg gtcacctgga
      481 caaagtcttc gtagaaaaga acatgcgaat caaagatctc atttactata tagttacgtc
      541 gaaagcgtat gcgcgtcgca cttctgctcc aggagattca gatccttctt cgattgctta
      601 ctttcaggcg acgcgactcg atccggatca attgatgaat tcaattcttc gcgcgagcgg
      661 ttcgctcgac gtaaaggcag gagacctcag ggaacgctcg attgcaggtg cggatcgatt
      721 ttcggggcgc ggtaagttgc taaagactga ggagaacgcg aaagaaatac agaacgcata
      781 ccaggttcct cgtcccgcag acgatcgttc tattctcgca gtgtttggtt caggagatag
      841 aatggacatc gcagacgatg acaaatcgat cacagtggag caggtgctga ctttactcaa
      901 tggcagattc accggcagac tctcctggga ttacggcaaa gcaggttctg ccgtggctgc
      961 cacctatgat cgcacaaagg acatgaacca ggtatatgat actgtcttcc tgagtgtgct
     1021 ttcacggccc gcgaaggcag aagagaaaga gcgccttcgc gtgctctctg ctgaaaaagt
     1081 aggaagagag aagggggaat accgacctga atttttacaa gatgtagtgt gggccatgtt
     1141 caatagccag gagttccttc atgtgcgctg atcggtttta tcccagaggc cctgcgattg
     1201 tttcaggcaa gagagaagga atgcaatctc gcaaagaatt tttgcagaaa tcggcggccc
     1261 ttctgggtgc tttctttctg ccagggcatt tgatgcccga agaggactat gaagacatca
     1321 ccgtcccttc ttccgtcaaa tctgtgatct tcctaaacat ggcaggtgga atgagccacg
     1381 tagatacgtt tgatccaaag gccggacaat ctgtgtttcc tggagtggcc acttccattt
     1441 ccggaattca atttgcagaa accatgaaag ccacggcaaa agaggcgtcg cgtgtttctc
     1501 tcattcgaag tacgtggtct gaagacggag atcatagctt tggacagaaa cttcttcata
     1561 ccggatacag agtcccgcag gcgcaggcgt ttccagacat tccatctttc ggtgccatca
     1621 tcgcgtatgc gaaaaagaga aaggacaaag gcccttactt tccttcgcac ataaccatgg
     1681 gcagtcgagg cggactgacg ggcaggtccg gctttttagg cgttaagtac ggcgcgtttc
     1741 aaattggaaa cctggacaat ccagtcactc atctaaaacc ggctgtaggc agaatcgcag
     1801 aagatagaaa aggaagacga ggcaagatgc tcgacatgct caacgaatcg ttttcaaaag
     1861 atacctcttc tgctcaggta gaccagtgga agcaaatgta tctcgctgct cttgattttg
     1921 ccaattcaga acgccttgac gtgtttgaca taagcaaaga gaagccagag cttcgcgcga
     1981 agttcgggga aagctcgatt ggaaaatcac ttctgatggc aaagagactc gctcaagcgg
     2041 aagttccatt cattgaactg acagtgggtg gatgggatac gcacaatgac aatgcggcca
     2101 agatcaaggg catcacgaag gatctagatc cagctcttgc agcacttcta ggagagctgg
     2161 gaagttctgg tttgctcaaa caaacgttag tcgtcctttc tagcgagttt ggtcgcacac
     2221 cagatgttgg gacgagagac ggaagagacc actggccaaa agtctggact actctgatcg
     2281 caggtggcag cattcctcag ggtgcagtca taggcgaaag tgatccaaaa ggacaaaagc
     2341 cggcaaaaag accggtccat gtgcgcgaaa tcgtggccac catctatcgc gcgtcaggag
     2401 tggatccgga tgcgcatccg tataattcta tgggtagacc ttttccgctt gtgcctcgcg
     2461 ggacagaggc agtcagtgag cttgtgggga attgagaatg aagatgaaat tctggactcg
     2521 cgctggtttg attctgggcc tattgtgcgc catcggtgta ctcgcaaacg agacggccaa
     2581 tcgaaagcgt catcctctgg atctggtgta tgagaaattc gcaggcacgt ccatcaagac
     2641 ggcagacccg gatactgtcc ttcgcaggat gtctttgcat cttcgcggaa ccattccagg
     2701 cacaaaagaa gttctggcat acgagaaagc agatcctgaa acaagagatc tgagtttcgc
     2761 cgtagacctt ctcaggtctc ctgacttcgc aggttactgg ggttcttatt ttgacagtct
     2821 atttcgcggc cagaccactg cgtggaaagt tccacaggcg gctttcttca aatacattgc
     2881 tgaatctctg cacgcgaata aaccgtatga tgttctagtc cgcgaaatga ttacgtcgag
     2941 cggatcgcct gataaaaatc cagcagtata cttctacgtg cgcgatggcg ccgatcccct
     3001 gcaagtggca gaatatgtag gtcgagtgtt tgatgccaga agagtaggtt gcgctcgctg
     3061 tcatgatcat ccgtttgaca caggatttac gaggcgtgat tactacgctc tgggcgcatt
     3121 cttt
//
LOCUS       DAOQJL010000475         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_20736_length_3117_cov_4.927172, whole genome shotgun sequence.
ACCESSION   DAOQJL010000475 DAOQJL010000000
VERSION     DAOQJL010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20736_length_3117_cov_4.927172"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLG78_16505"
     CDS             <1..483
                     /locus_tag="PLG78_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24776.1"
                     /translation="KKYKKEFQIIVGGWHADYPDPQNFLQLLYGPNTASSYNEGSYRN
                     PTYDSLYLQMKNMPPGPARLAIIRKMKEIVADDTPVIMQFHPITYGLSHPWFSPFKTH
                     PTNSNQLKFRDLDPEKRKTLVAQWNRMPLAGYLLFAFLILVLVLPVVIAVRKYSRRLR
                     "
     gene            483..1466
                     /locus_tag="PLG78_16510"
     CDS             483..1466
                     /locus_tag="PLG78_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE24777.1"
                     /translation="MLTYIIRRIIYTLPIWIGVYGITFALFHMRDPIAIARVHMPQAP
                     DAALKAWVRNNNLHLPRFVNLPADADTVLVDGKKHPELASKSIFYSQFFLGFREIITF
                     DFGVDKNRQPISESIAERAPYSLSVMLPAFLITVTLSVVMSLFLAYFRETGLDVGAVL
                     ITVAMMSIPVPVYLLLCNWGFGKYLKLVPVYNHVLLPILVSVLAGLGGQVRFYRTVFL
                     EQIEQDYIRTARAKGVSESGILFKHVLPNSLIPILTSVVMSLPFLITGSLILEHFFGI
                     PGMGDMMYTAIYSQDFQVIKVMVYLGAFLYMIGALLTDISYILADPRVVLE"
     gene            1463..2494
                     /locus_tag="PLG78_16515"
     CDS             1463..2494
                     /locus_tag="PLG78_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE24778.1"
                     /translation="MITILIDIAWILAAAAGIVALRSFLRNPSLKDARDMLASRLRVR
                     IAFYVVCAYLGVGLIGQIKVPEKERIADRSLLDLSLQWIQKERTYSAPFASEVTGIRM
                     LEADRELNRVKGIHILGTDINGVDVMHNVLRGASTALILSFGTALVAMPLGVLLGILA
                     GWSGGKLDDLIQWLMSTVASIPWLLFVIAFLMVFGRGLFWICLAIGLTSWVGTARLIR
                     GETLKQKSLDYITAARATGLSVPRILFRHLLPNVRYLIIIDLTLFCSSVILAESILTF
                     IGIGVEQGTASWGAMLIESEKELMRSPAIWWVFAGSAFCGILPLVLSLNVLGDALRDA
                     LDPRAVSSR"
     gene            complement(2491..>3117)
                     /locus_tag="PLG78_16520"
     CDS             complement(2491..>3117)
                     /locus_tag="PLG78_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE24779.1"
                     /translation="RVGEEQIHHATDAMARIQDTAGKITEFVGIITEISDRTNLLSLN
                     AAIEAARAGDAGRGFAVVAQEITKLADRTLESAKEVTTLIHAALDSIKKGTVQVDSVA
                     GNLGGIVGDVQKIDRFAEAVMERISLQVIDTQNISDNASGLTATSTDMLTSLTEQKRA
                     TREIESTIGNVATTAQTVSSGTHELRTLATQLREKSEELLGTASRFQI"
BASE COUNT          637 a          765 c          804 g          911 t
ORIGIN      
        1 aaaaagtaca agaaggaatt ccagatcata gtcggtggct ggcacgcgga ctatcctgat
       61 ccgcagaatt ttctacaatt gctctatggc ccgaacacag cgagttcata caatgaggga
      121 agctacagaa atccgactta tgattctctc tatttgcaaa tgaagaacat gccacctgga
      181 ccggctcgcc tggccatcat tcgtaaaatg aaggagattg tcgcagacga cacgcctgta
      241 atcatgcaat ttcatcccat cacttatggg ttgtctcatc cgtggttttc tccgttcaaa
      301 acgcatccta cgaattcaaa tcaattgaaa tttcgcgatc ttgatccaga gaagcgcaag
      361 actcttgtag cgcagtggaa ccggatgccg ctggccggat atttgctgtt cgcatttctc
      421 attctggttc ttgtgttgcc cgttgtgatc gccgtgcgaa agtattcgag gagacttcgc
      481 tgatgctcac gtatatcatt cgccgcatca tctataccct tcccatctgg attggagtat
      541 acggaatcac cttcgcgttg tttcatatgc gtgatcccat tgcgattgcg agagtccaca
      601 tgccgcaggc gccggacgcc gctctcaaag cctgggttcg caacaacaat ctgcatttac
      661 caaggtttgt gaatcttcct gcagatgcgg acacagtgct ggtggacggc aaaaaacatc
      721 cagagctcgc ttctaagagc attttttatt cgcagttctt tcttggcttt cgtgagatca
      781 tcacgtttga ttttggagtg gataagaatc gccagccaat cagtgagtcg attgcagagc
      841 gcgctcctta ttctctgtca gtgatgttgc ccgcattctt gattacagtc acactcagcg
      901 tagtgatgtc cctgtttctt gcgtacttca gagagacagg tctggatgta ggcgccgttc
      961 tcattactgt tgcgatgatg agcattcctg tgcccgtcta tctgcttctc tgcaattggg
     1021 gctttggtaa gtacttaaaa ctggttcccg tgtacaatca cgtgttgctt ccaatcttag
     1081 tctcggtgct tgcaggactg ggtggccagg tgcgctttta tcgcaccgtc tttctggagc
     1141 aaatcgagca ggactacatt cgaaccgccc gagcaaaggg cgtaagcgag agcggcatcc
     1201 tgttcaagca tgtattacca aattctttga ttcccattct gacttctgtg gtcatgtctt
     1261 tgcctttctt gatcactggc tcactgattc tcgaacattt ctttggcatt cctggaatgg
     1321 gtgacatgat gtatactgcc atctacagtc aggatttcca ggtcatcaaa gtgatggtct
     1381 atctgggtgc gttcctctac atgattggcg cactcctcac ggacatttcc tatattctgg
     1441 cagaccccag ggtggtgctc gaatgatcac gattctgatc gacattgcct ggattctcgc
     1501 ggcagcggca ggcatcgtgg cattgaggag tttcttgcga aatccttctt tgaaggatgc
     1561 gcgtgacatg ctcgctagcc gcttgcgagt gcgcatcgcc ttctacgtag tatgcgctta
     1621 ccttggtgtt ggcttgattg ggcaaatcaa agttccagag aaagagagaa tcgcggatcg
     1681 cagcctgctg gatttaagtt tgcagtggat tcagaaagag cgcacataca gcgcgccttt
     1741 cgcatccgag gtgaccggca ttcgcatgct cgaggcagac agggagctca atcgagtcaa
     1801 aggcattcac attctaggaa cggacatcaa cggtgtggac gtaatgcaca acgttcttcg
     1861 cggtgcaagc acggcgctga ttctgtcttt tgggacagct cttgttgcca tgccgctcgg
     1921 tgtgcttctg ggaattcttg ctggttggtc aggcggaaag ttagacgatc tgattcaatg
     1981 gctcatgtct acggtggcgt ctattccctg gcttctcttt gtgattgcat ttctcatggt
     2041 gtttggtcgc ggactttttt ggatttgtct tgcgattggc ctgacttcgt gggtgggaac
     2101 ggcccgattg attcgcgggg aaactctcaa gcagaagagt ctggactaca tcacagcagc
     2161 cagagcaacc ggtctttccg ttcccagaat cctctttcgc catttgctcc cgaacgtgcg
     2221 atatctgatc atcattgatc tgactctgtt ctgttcgtcc gtgattcttg cagagagcat
     2281 cctgactttc atcgggattg gagtggagca gggcacggcc tcctggggcg ctatgctcat
     2341 cgaatcggaa aaggagctta tgcgatctcc ggccatctgg tgggtttttg ccgggagcgc
     2401 tttttgcgga atccttcctc tggtcctgtc tctaaacgtg ctcggggatg cgcttcgcga
     2461 cgcactggat cctcgcgctg tgagttcgcg ttagatctgg aagcgcgaag cagtacccag
     2521 gagttcttct gatttttcac gaagttgcgt ggcgagagtg cgaagttcat gagtgcctga
     2581 gctgactgtt tgtgccgtag ttgccacgtt tccaattgtg ctttcgattt cgcgtgttgc
     2641 tcttttttgt tctgtcaggc tggtcagcat gtcggtgctt gtggctgtaa gtccgctcgc
     2701 gttgtcagaa atgttttgtg tatcgattac ctgcaggctg atgcgctcca tgaccgcttc
     2761 tgcgaaacga tcaatttttt gaacatcgcc cacaattcca cccaggttgc cggcaacaga
     2821 atctacctga acggttccct tcttaattga atcaagggcc gcgtgaatga gagtagttac
     2881 ctcttttgcg ctttccagag ttctgtctgc gagtttggta atttcttgtg caacgactgc
     2941 gaatcctcgt ccggcatcgc ccgcgcgtgc agcttcaatg gcagcattga gagagagcaa
     3001 gtttgttctg tctgagattt cagtgatgat tccaacaaat tctgtgattt ttccggcggt
     3061 gtcctggatt cgtgccattg catctgttgc atggtgaatt tgctcttcgc ctacgcg
//
LOCUS       DAOQJL010000476         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21014_length_3092_cov_4.886401, whole genome shotgun sequence.
ACCESSION   DAOQJL010000476 DAOQJL010000000
VERSION     DAOQJL010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21014_length_3092_cov_4.886401"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLG78_16525"
     CDS             complement(<1..845)
                     /locus_tag="PLG78_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNE24780.1"
                     /translation="MKQPSTEWREDIASDEDQRFTQYANRFIEIQKQNSAKYGNGRGL
                     HRKQLLGLNATFEVLGDLPAYAKHGLFAQPRKYDAWVRLSNGGMRVQEDSKPDIRGFA
                     LKVRGLKGPGALGTETNCQDFLLINLPAFSSPKSAEFVALVSHLAQGPLSVLGYLFKT
                     YGFSGALARMKKAKQSFGRPFTGFATEKFYTAAPIKCGPYACRVRMLPASDKPDPNAS
                     QSWAKDIQKRVSSGALVHDFQLQFFVDEKTTPVEDASVDWPESESPYVTVARLTIPQQ
                     AVEGP"
     gene            complement(883..1962)
                     /locus_tag="PLG78_16530"
     CDS             complement(883..1962)
                     /locus_tag="PLG78_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24781.1"
                     /translation="MKTVKKIFLGIAAVLAVLLVYVFVSKAITDRKMERETAMPEGGI
                     SSLESVTIGGISQWILIRGEKKDNPILLYIHGGPGSTEMFMSRKMDTEIVKDFVVVHW
                     DQRGAGKSYSPFIPSASYNREQYLSDTYELVSLLKKRFSRNKIYIMGHSWGSYLAAVT
                     AHRHPEDFCAFVGIGQMVNAVENESVSYDFALKRALIEENKEAFNDLLEIGKPPYKTL
                     GQMMTQRKWLTHFGGGMFHGEHSKDGFSYMGSFMSESPDYSLLDSVRFFLGMITTLRN
                     VWGEFFTLDLYTQAPSFKVPVYFMTGRHDYNTPFEILVRFEKDLQAPKKKIYWFEQSA
                     HSPNFEEPAAFAQALREIKSDGCKQ"
     gene            complement(1985..3019)
                     /locus_tag="PLG78_16535"
     CDS             complement(1985..3019)
                     /locus_tag="PLG78_16535"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24782.1"
                     /translation="MKRFVSTCLCLLTTLSMDCAQKPSPLSIDWGLIAIAASIVKIPR
                     FVFVTSNTGNVYVYSINPFVCSLNPLSPMWAPASPGIPAIDSTGQFLFVPGSVANQIA
                     SYRINTTTGVLTQVGFVSTTSPTALAYDPAVGQVLSVSTGSSMISRFALMPDGSLTSA
                     GSVAMPAPASGNIAIDTGSRIAFVPHGISLFSAASLAAGVASSSSAPAVATSAALNGG
                     YLYVGGSTTILTYRLNGTNAPGFVSTTPSAGTLTSLAADSQGANLYGADATGVLNRYA
                     IQNGGTLLLNSQSLFGPSANSIAVDPTGTCVVMSGGSNVRAFRTSSAGLTLFAAGFVS
                     NPNAVLIASY"
BASE COUNT          789 a          783 c          793 g          727 t
ORIGIN      
        1 tcagggcctt ctaccgcttg ctgaggtatt gtcaatcttg ctacagtcac ataaggcgat
       61 tccgattccg gccaatccac agatgcatct tctactggag ttgttttctc atccacgaag
      121 aattgcagct ggaaatcatg tacgagtgca ccggaagaaa ctctcttctg aatgtctttg
      181 gcccagctct ggcttgcatt cggatccggc ttatcactgg caggaagcat acgcacgcga
      241 caggcatacg gaccacattt gatcggagcc gcagtgtaga atttctcagt cgcaaatccg
      301 gtaaacggtc ttccgaatga ttgctttgcc ttcttcatgc gagcgagagc tccactgaaa
      361 ccatatgtct tgaataagta tccgagaaca gacagaggac cctgcgcgag atgcgaaacg
      421 agagccacaa attctgcact tttgggagaa gagaaagcag gcaaattgat caaaagaaaa
      481 tcctggcagt ttgtttctgt cccaagagca ccgggacctt tgagcccgcg caccttgagt
      541 gcaaagccgc gaatatccgg tttcgaatct tcctgaactc tcattcctcc attggataga
      601 cgtacccatg cgtcgtactt gcggggttgt gcaaacagtc cgtgcttcgc ataagccggg
      661 agatctccaa gaacttcaaa cgttgcgttt aaaccaagaa gctgtttgcg atggaggcct
      721 cgaccatttc catactttgc tgagttctgt ttctgaattt cgatgaagcg atttgcgtac
      781 tgcgtaaatc tctggtcttc atccgaagca atgtcttcgc gccattctgt gctgggttgt
      841 ttcatgggct gagtagacaa cccggccccc gcgtagcaag cattactgct tgcagccgtc
      901 ggatttgatt tcgcgcagag cctgcgcaaa ggcggcgggc tcctcgaagt ttggagaatg
      961 cgcggactgt tcgaaccagt agattttctt ctttggcgcc tgcagatcct tctcaaagcg
     1021 cacaagtatt tcaaacggcg tgttatagtc atgacgccct gtcatgaaat agaccggaac
     1081 cttaaaagac ggagcctgag tgtagaggtc cagggtaaag aactcgcccc agacatttcg
     1141 cagagtggtg atcataccca ggaagaatcg aaccgaatcc agaagagagt agtccggcga
     1201 ttcagacatg aaacttccca tgtaagaaaa tccgtctttg ctgtgctccc catgaaacat
     1261 accgccgcca aagtgagtca gccacttgcg ctgggtcatc atctggccca gagtcttgta
     1321 gggaggcttt ccaatttcga ggagatcgtt gaatgcttct ttgttttcct ctatgagcgc
     1381 tcttttcaat gcgaagtcgt aagacacact ttcgttttcc actgcattga ccatctgacc
     1441 gattccaaca aatgcgcaaa aatcttctgg atgcctgtgc gccgtcacgg ccgccaggta
     1501 cgatccccag gaatggccca tgatgtagat cttattgcgg gaaaatcgct tcttgagcag
     1561 agataccagt tcatatgtat cgctcaaata ttgttccctg ttgtaggaag cagaaggaat
     1621 gaaaggagaa taagatttgc ctgcgcccct ctgatcccag tgaactacta caaaatcttt
     1681 aacaatctcc gtatccatct tgcgagacat gaacatttcc gtcgatccag ggccgccgtg
     1741 aatgtatagc agaatggggt tgtccttttt ttctccacgg atcagaatcc actgagaaat
     1801 gccgcctatc gttacagatt caagagaact gattccgcct tccggcattg cggtctctct
     1861 ttccattttg cgatccgtga ttgccttgga gacaaacacg tacaccagaa gcacggcaag
     1921 cactgccgca attccaagaa agatcttctt tactgttttc attttcaatc ctgaacgtag
     1981 agagtcaata ggatgcgatg agaaccgcgt ttggatttga gacgaacccg gcagcgaaga
     2041 gcgttaagcc tgcagaagaa gttcgaaagg ctctaacatt tgaaccgcca gacatgacaa
     2101 cacatgttcc ggtgggatcg actgcaatgg agttcgcgct tggaccaaat agcgactgcg
     2161 aattgagtag gagcgttcca ccgttttgaa tcgcgtagcg attcaaaacg cccgttgcgt
     2221 ccgctccgta cagattcgct ccctgagagt ctgcggccag ggaagtaaga gtgcctgcag
     2281 aaggagtcgt actcacaaag ccgggtgcat tcgtaccatt caaacgatac gtgagaatgg
     2341 tagtgctgcc gcctacgtac aaataaccgc cgttaagcgc tgcactcgtg gcaaccgcag
     2401 gcgcactgga ggacgacgca actcctgccg cgagcgatgc ggcagaaaac aacgatatcc
     2461 cgtgcggcac aaaagcgatt ctagatccag tatctattgc aatgttgccg cttgccggag
     2521 caggcattgc aacgctgcct gcagaggtaa ggctgccgtc aggcatcagc gcgaaacggc
     2581 ttatcatgga ggaaccggtt gatacactga gaacctgacc tactgcggga tcataggcaa
     2641 gcgctgtggg tgatgtagta gagacgaaac ccacctgggt gaggaccccg gtggtagtgt
     2701 tgatacggta gcttgcgatc tgattggcca cgctcccggg cacaaagaga aactgtcctg
     2761 tagaatcaat ggcaggaatc ccgggggagg cgggcgccca cattggggac aagggattga
     2821 gcgagcaaac gaacggatta atcgagtaaa cgtaaacgtt acctgtgttg cttgtgacaa
     2881 atacgaagcg cggaatcttc actatggagg cggcgattgc aatcaacccc cagtcaatcg
     2941 acagcgggct tggcttttgt gcacaatcca tggacagggt cgtgagcaag cacaggcaag
     3001 tagaaacgaa gcgcttcacg gtaccgatca tagggcgcgc actcagacat tcaagtaaaa
     3061 cttgttgcgc gcatcccacg gaccattctg ct
//
LOCUS       DAOQJL010000477         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21147_length_3081_cov_4.021811, whole genome shotgun sequence.
ACCESSION   DAOQJL010000477 DAOQJL010000000
VERSION     DAOQJL010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21147_length_3081_cov_4.021811"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..227
                     /locus_tag="PLG78_16540"
                     /pseudo
     CDS             1..227
                     /locus_tag="PLG78_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            236..1063
                     /locus_tag="PLG78_16545"
     CDS             236..1063
                     /locus_tag="PLG78_16545"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNE24783.1"
                     /translation="MSALVIDIQDIGVRYYTFATTMRYAFETIHEAGLDLPVYVIDHP
                     NPCGQSVEGIPLDATFESFVGPVGLPHRHGLTIGQLARFFHQELRAHFPLHVVAFDPE
                     DEGLPFRISPSPNMPGPYTHLVYSGQCLLEGTNVSEGRGTTRPFEIFGASYMEKLHSK
                     RPPTAPGAVLRPVRFIPTFHKDAGKICYGYQIHVTGPYHSLLHSLQLIRWIRSECHEF
                     RWNEGPYEFRSDRPAIELLAGHRVLLSYLNGETDLPHAEAAIQAGEHEWKNRIRSLS"
     gene            1137..>3081
                     /locus_tag="PLG78_16550"
     CDS             1137..>3081
                     /locus_tag="PLG78_16550"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE24784.1"
                     /translation="MLRCRFIWIIPVILGLLVCKKVEPPHAVQGEMDLSTHDWSSQGP
                     VSLQGMWRFYWKEFRDPAIPLSGSSFIQAPGTWNEFDSGSGKAGGAGYATYALRILSK
                     PDADLALRIQYFDSSYRLYANGTLLAQAGVAGTTAENSIARRLPTTVRLMPHNGSIDL
                     VIHVSNFAHRNGGFPRELILGSPADIELIRTHKIMLDLFMAGAIIMMGLYHLALFFLR
                     RSDYSPLYFGLFCILIALRTDLVGERTIWGLTGSFGSFALSHRIEYATFYLSVPLFFA
                     YTRSLFPDSYFRKDILLTAAAIFGSATLLTFFVESYIYTQWLWIFQAALGFIGIYILV
                     GVCKMVIVRRRGARVFVGTFVLLFAAAINDVLYSRRLIESGFFIALGLFVFIFTQAFL
                     LSRLYAESFRRVEELAIDLTNSERRYRHLVEDSGEIILSLDSSGKILTSNKTSSRILG
                     YSDISGRTLASLVFEQGQSNVFLARHVMEERLASLIQTGRADEFPAHFKTAQGDLCEL
                     NVRLQRVNLEGGMTILANLAQKPLDLLARYCLEDHRHYVLFNSFHMGELLNQSLTAGL
                     EARVSEEEQMLVRLALREMMINAIEHGNLGITFEEKTKATASGTLAELVSERLKNPYY
                     SARKLHVRVDSSEDRIVFSIRDEG"
BASE COUNT          726 a          796 c          779 g          780 t
ORIGIN      
        1 ccggcatcgt ctgggcggtc ggctccaagg tgaagcgctg gaaggtcggc gacgaggtcg
       61 tcgtccactg caaccaggac gacggcgacg acgaggagtg caacggcggc gacccgatgt
      121 tctcggcgtc gcagcgcatc tggggctacg agacgccgga cggctccttc gcgcagttct
      181 gccgggtgca ggcgcgccag ctgctgccgc ggccgcagca tctcacctga aagatttgtc
      241 tgcgcttgtc attgatatcc aggacatcgg ggttcgctac tatacatttg ccacgaccat
      301 gcgatatgca tttgagacga tccacgaagc aggattggat ttgccagtct acgtcatcga
      361 tcatcccaat ccgtgtggcc agagcgtaga aggaattccc ctggatgcca catttgaatc
      421 ctttgtcggg ccggttggac ttccacacag gcacggtctg acgattggcc agctggctcg
      481 tttctttcac caggaacttc gcgcccattt tccgcttcat gtggtcgcct tcgacccaga
      541 agacgaaggt ctaccgtttc gcatttcgcc ttcaccgaac atgcctggcc cgtacactca
      601 tctggtttat tccggacaat gcttgcttga ggggaccaac gtaagcgagg gcagaggcac
      661 aaccagacca tttgaaatct ttggtgcttc ttatatggag aaacttcact caaagaggcc
      721 acccacggca ccaggtgccg tcctccggcc ggttcgtttc atccccacgt ttcacaaaga
      781 tgcgggcaag atctgctacg gttaccagat tcacgtaaca gggccgtatc actctcttct
      841 tcactctttg cagctcattc gctggattcg cagcgagtgc catgagttcc gctggaacga
      901 aggtccctac gaatttcgat cggatagacc cgccattgaa cttctagcgg gtcaccgcgt
      961 tctattgtcc tatctcaatg gtgaaacaga tctcccgcat gcagaagcag ccattcaggc
     1021 aggggaacat gaatggaaaa atcgaattcg ttctttatcg tgaaaccctg gacgagcatt
     1081 gtcgcaggca atctgaattg aattgacctt tctggatcaa tcacctactc tcggatatgc
     1141 taagatgccg gttcatctgg atcattccag tgattctggg cctcctcgtt tgcaagaaag
     1201 tagaaccacc ccatgcagtt cagggcgaaa tggatctgag cactcatgac tggagttcgc
     1261 agggtccggt ttccctccag ggaatgtgga gattctactg gaaagagttt cgtgatcctg
     1321 caattcctct cagcggttcc agtttcattc aggcccccgg cacgtggaat gaattcgact
     1381 cgggttctgg aaaagcgggc ggtgccggtt atgccaccta cgcgctgcga attttatcca
     1441 aaccggatgc ggacctcgcg cttcgcattc aatacttcga ttcgagttac aggctgtatg
     1501 cgaatggcac gttgcttgcg caagcaggtg tcgctggaac gacagcagaa aacagcatag
     1561 cccggagact ccctaccaca gtgcgattga tgccgcataa cggttcaatt gatctggtga
     1621 ttcacgtttc caatttcgca catagaaacg gaggctttcc gcgtgaactg atcctgggga
     1681 gtccggcaga tattgaactc atccgaactc acaagatcat gctggatcta ttcatggcag
     1741 gtgccataat catgatgggt ctctaccatc ttgctctttt ctttttaagg cgcagcgact
     1801 attctcctct ctactttggc cttttttgca ttctcatagc actgcgcacg gatctggtgg
     1861 gggaaagaac catctggggc ttgacgggtt catttggatc ttttgcgctc agccatagaa
     1921 tagaatatgc tactttctat ttgtccgtcc cgctgttctt tgcgtataca agaagtcttt
     1981 tcccggattc gtatttcagg aaagacatac ttcttacggc tgcggccata ttcggaagtg
     2041 cgactctcct cacattcttc gtagaatcct acatatacac tcaatggctc tggatcttcc
     2101 aggcagcact cggattcatt ggcatctaca ttctggtcgg agtatgcaaa atggtcatcg
     2161 tgcgcagaag aggcgcaaga gtgttcgtgg gcacctttgt gttgttattc gcagcagcga
     2221 tcaacgatgt actctacagt aggagactca tagaaagcgg attctttatt gctcttggtc
     2281 ttttcgtatt tatctttact caggcattcc tgctttcgcg tctctatgct gaatcattcc
     2341 gaagagtaga agaactggcc attgacctca ccaattcaga aagacgatac aggcatctgg
     2401 tggaggactc aggggaaatc atcttgtccc tggattcgtc aggaaagatt ctcacatcta
     2461 acaagacatc ttccaggatt ctgggatact ctgacatttc cggtcgcact cttgcaagcc
     2521 tggtatttga acagggccag agcaatgtgt ttcttgcccg gcatgtgatg gaggaaaggc
     2581 tggcgagcct catccaaaca ggaagggcag atgagtttcc agctcacttc aaaacagctc
     2641 agggagacct ctgcgaactc aatgtgcgcc tgcaaagagt gaacctggaa ggagggatga
     2701 cgattctggc aaatctcgca caaaaacctc tggatctgct cgcgcgttat tgcctcgaag
     2761 atcacagaca ctatgtactt tttaatagtt ttcacatggg agaattgctc aaccagtcac
     2821 tgactgccgg gttagaggcg cgcgtttcag aagaggagca gatgctcgtg cgtctggctc
     2881 ttcgcgaaat gatgatcaat gcaatcgaac acggaaacct tggaattact ttcgaagaaa
     2941 aaacaaaagc aactgcaagc ggaacactcg cagaacttgt atccgaacgc ttgaaaaatc
     3001 cttactactc cgcaagaaaa cttcatgtgc gcgtggactc aagtgaagac aggattgtat
     3061 tctccattcg agatgaaggg g
//
LOCUS       DAOQJL010000478         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21212_length_3076_cov_3.691493, whole genome shotgun sequence.
ACCESSION   DAOQJL010000478 DAOQJL010000000
VERSION     DAOQJL010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21212_length_3076_cov_3.691493"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG78_16555"
     CDS             <1..317
                     /locus_tag="PLG78_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24785.1"
                     /translation="VEELIGEFAEASNRIMEKFPVEINTRTLERESLVQANAQFRNLK
                     HILMEIEESLREMEQNLFEKDSPRAVRYVTKFKKDVVNDINTIMFKINGRISDAVNGI
                     HG"
     gene            complement(286..1770)
                     /locus_tag="PLG78_16560"
     CDS             complement(286..1770)
                     /locus_tag="PLG78_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24786.1"
                     /translation="MRPVYWIILVLTVFFAADSLIFRAGIYTLPNESAWETEPFYNLE
                     SAMHHLRTKSPDEFRILIVGSSLAMYGFLYERMEERMSGLAPGKKVKVFIVAHQGMHG
                     LELAALTDRYAALKPDLVILPVNMVDLRLERPILMSLMEDLGGTRRKEALDILYRDLA
                     GMYAVLPLAGQGMVEHYGRHLNVLQIGKVGLFGLCEGCRLKLFLPGPASTFFDNRFSR
                     GKSYDRFAGGRVEYNGEVAVTHRGHVPSNFAFRITPNQIARGMDLEIMQDGSVVAIRN
                     SLKSLKKEVQVSRGWVHFDLKGLGTVGDVLQVQISTSVYMDVFDDRYAARLTRNFATD
                     QPLLAGPRPRRREDELLDRMDDATYRKSFQIRNLAFEKPGYQYLKALYDAKKVWGTRA
                     FDPDLPAIAGVGDFVRRMPADTIVLNFPENPLTLSMYGQTRYYRDYVKYLQAIAAPGK
                     FVDASSVLPARKFYDYHHLTWYGAGEFSDYMSDWISREYHSRHH"
     gene            complement(1774..>3076)
                     /locus_tag="PLG78_16565"
     CDS             complement(1774..>3076)
                     /locus_tag="PLG78_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE24787.1"
                     /translation="RVFRLLRLARMARIAQFMRKLAQADIINISVLRMLFLTFWVLMV
                     AHWSACVWIALGGTIGGVPDDEKNSLLTIYVRSLYWATTTITTIGYGDLTPGTTKQTI
                     FTMLIQLTGAGLYGYVIGNIASLIANLDVARAQYLEKLEKIGTYMKFKKLPHAMQQEV
                     RNYYTYLWDTRRGYDESQVLVDLPKPIVEKVSMYLLQQMIEKVPIFKGANEDLIRQIV
                     IALKPEVYTPGDYIFRKGDMGGTMYFIGKGEVQVVGDDGKTVFATLSEGNFFGEIALL
                     MSMPRTASIRAVDFCDLYSLEKETFNGIVEHYPDFSAHILELARARAREIGNRDTVTS
                     TEAIHKPPRPARVVGLTASRVDDALRLSWEGVDTAAVYQVIRKGEGRWTMVNGFVQNT
                     VLIDHPLTGLSAYRVRAANESGPGDWSDVIEVAMSATSKNP"
BASE COUNT          812 a          844 c          715 g          705 t
ORIGIN      
        1 aagtagaaga actcataggt gaattcgcag aggcatccaa caggatcatg gaaaagtttc
       61 cggtggaaat caacactcgt actctggaga gagaatccct ggttcaggca aacgctcaat
      121 tccgcaatct caagcacatt cttatggaaa tagaagagtc ccttcgcgaa atggaacaaa
      181 acctctttga aaaagacagc ccccgtgcgg ttcgctatgt gaccaaattc aagaaagacg
      241 tggtcaatga catcaatacg atcatgttca agatcaacgg tcgaatcagt gatgccgtga
      301 atggtattca cggctaatcc aatcagacat ataatctgaa aactcaccgg cgccgtacca
      361 cgtaagatgg tgatagtcgt agaattttcg cgccggtaaa acggagcttg cgtccacgaa
      421 ttttcccgga gccgcaatcg cctgaagata cttcacatag tcgcgatagt agcgcgtctg
      481 cccgtacatc gaaagagtca aaggattctc cggaaagttc aagacgattg tgtccgccgg
      541 cattcgccgc acgaaatctc caacacccgc gatagcagga agatcaggat caaacgcccg
      601 agtgccccat accttctttg catcgtatag agcctttaaa tactgatagc ctggtttctc
      661 aaatgccaga ttgcgaattt gaaacgattt tctgtatgtc gcgtcatcca ttctatccag
      721 tagttcatct tcgcggcgac gcggcctggg ccccgcaagc aaaggctgat ccgttgcgaa
      781 attgcgcgtg agtctggccg cataacggtc atcaaataca tccatgtaca cagacgtaga
      841 aatttgaacc tgcaaaacat cgccgacggt tcccaggcct ttcaaatcaa aatgaaccca
      901 gccccgcgag acctgaactt cctttttgag tgacttgaga gaattccgaa tagcaacaac
      961 cgaaccatcc tgcatgatct ccagatccat tcctctggct atctggttag gtgtgattct
     1021 aaaggcgaag ttcgaaggaa catgcccgcg atgggtaacc gcaacctctc cattgtattc
     1081 tactctaccg cccgcgaatc ggtcgtagct cttcccgcgc gaaaagcgat tgtcaaagaa
     1141 agtggaagca ggaccaggca gaaatagctt caatctacaa ccttcgcaca ggccgaaaag
     1201 ccccaccttg ccgatctgca gcacattcag gtgcctgcca tagtgctcga ccatcccctg
     1261 gcctgccaga gggagcaccg catacatgcc tgccagatcg cgatacagga tatccagcgc
     1321 ttcctttctc ctggtgcctc ccagatcctc catcaaactc atgagaatgg gacgctccag
     1381 cctcaggtcg accatattga cgggcaaaat cacgagatcc ggtttcaatg ccgcataacg
     1441 gtccgtaagg gccgccagtt ccaatccatg catcccctga tgagcaacga tgaacacttt
     1501 gactttcttt ccgggcgcaa gcccggacat tcgctcttcc attctctcgt acagaaatcc
     1561 gtacattgcc agagagctcc ccacaatcag gattcgaaac tcatccggag atttagtccg
     1621 cagatggtgc attgctgatt caaggttgta gaatggttcc gtttcccacg cactttcatt
     1681 gggaagcgtg tagatgcccg cgcggaatat gagggaatcc gccgcaaaaa acacggtcag
     1741 taccagaatg atccagtaaa cgggacgcaa caattacgga tttttagacg tggcggacat
     1801 tgcaacttca atcacgtcgc tccagtcccc gggacctgat tcgtttgctg cgcgaacacg
     1861 ataggcgctg agccccgtca gtggatgatc aatcaaaacc gtgttctgga caaagccatt
     1921 taccatcgtc cagcgaccct cgcctttgcg aatcacctga taaaccgccg cagtgtctac
     1981 gccttcccag ctcaaacgaa gagcgtcatc cacgcgggat gcggtaagtc ccacaacacg
     2041 cgcgggacgt ggcggcttgt gaatcgcctc agtcgaagta accgtatcgc gatttccgat
     2101 ttcgcgggcg cgcgcacgtg caagctccag aatgtgtgcg gagaaatccg gatagtgctc
     2161 tacgattccg ttgaacgttt ctttttccag agaatacaaa tcgcaaaaat cgacagcgcg
     2221 aatacttgca gtccggggca tactcatgag cagagcaatt tcaccgaaga agttcccctc
     2281 actcagtgtt gcaaatacgg ttttgccgtc gtctcctacg acctgcacct cacccttacc
     2341 gatgaagtac atcgtcccac ccatatctcc ctttctaaat atgtagtctc ccggtgtgta
     2401 gacctctggc ttgagtgcga ttacgatctg gcgaatcagg tcttcgtttg cacctttgaa
     2461 aatcggaacc ttctcaatca tctgctgaag cagatacatg gagacttttt caacaattgg
     2521 ttttggtaga tcgaccagaa cctggctttc gtcatagccc cgtcgagtat cccagagata
     2581 ggtataataa ttgcgaactt cctgctgcat agcatgcgga agtttcttaa acttcatgta
     2641 cgtcccaatc ttctcaagct tctccaggta ttgagcacga gctacgtcca ggttcgcaat
     2701 caagctggcg atgttaccaa tgacgtaacc gtaaaggccc gcacctgtga gctgaatgag
     2761 catggtgaag atagtctgct ttgtagtccc aggcgtcaga tcgccatatc caatggtggt
     2821 gatcgttgtg gtcgcccagt aaagagagcg aacatagatc gtaagaagcg aattcttctc
     2881 atcgtctgga acgccgccta tcgttcctcc cagagcaatc cacacacagg cagaccagtg
     2941 cgccaccatg agaacccaga acgtcaagaa aagcattcgc aagacgctga tattgatgat
     3001 atctgcctgg gccagttttc gcatgaactg tgcgatacga gccatgcgtg cgaggcgaag
     3061 cagacgaaat actctc
//
LOCUS       DAOQJL010000479         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21263_length_3072_cov_5.384488, whole genome shotgun sequence.
ACCESSION   DAOQJL010000479 DAOQJL010000000
VERSION     DAOQJL010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21263_length_3072_cov_5.384488"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2668)
                     /locus_tag="PLG78_16570"
     CDS             complement(<1..2668)
                     /locus_tag="PLG78_16570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24788.1"
                     /translation="MTCIVCELIQTMHFEVLELSANFFDRFTPSVLTFILAAFLLSRR
                     QTAARYLGAFFLFLTVFNAGYLLGYSGIHAAAPYGFHLACFITIALVFKIQFAYRMPI
                     WIYRRESIVVFVVTMLASIAAIVEYSLSAESAGYRLIFDQHIFASRYASTYIPLGSMI
                     MFFWVAIVLLRQIDHLIPSPPWFEFSSLSPWFKWPLRVLLRPVIVLSKLFRAGSAQTQ
                     AVRSFFLLVLAELTINIIVLVGFLTSFVSKELVALLLNTGLLFVYFWYGMVYINHSRE
                     STSMMVRLVGIALVTLLVVTGLIGSRANQRTESAFDGGRKSALPGLALDLGERSFADT
                     RIPGDLQYVAYRPHPSRYSRDYRIIFAKSSLSSSAMADSDVEAREYELLRRANQLKRE
                     KGTSISAARELASNEMDRQIVELDHRLYRTAAGVPYLFYVYQGKQGLYEFGFLYSDYR
                     SEMHKTVTENVVIVFAVTLVILTVFPLLFQVNLIAPLNLLLAGVARVEKGDMEHEVPV
                     QVQDEIGFLARAFNAMVKSIRDAQARLKDYADTLEIKVQDRTRELQRAYDNLDDLKRK
                     QDGDYFLTSMLIDPLARNDGASERIAVEFLIEQKKKFEFKKWKSEIGGDMNIADRIVL
                     RGKPYTMVLNADAMGKSIQGGGGILVLGSLIHAILNRTKSGAAISDYTPERWLHQTFL
                     EMDSVYRSFNASMLVSLVLFLIDEQSGLAYYLAAEHPLPVLLRAGQASFLEYPHALNK
                     LGTPGVDVAAFIETIQLRVGDRIILGSDGRDDLILPAEDGTTRMNEDSELFCSVVQSA
                     QGDLPRIREMLGSTGEIIDDLSLACVSILQLRERMSKRAKKITLQDQIKVKNWKEGAV
                     TALSLLEDDPADLDFFPVAARICQKAGIVSQAI"
     gene            complement(2724..>3072)
                     /locus_tag="PLG78_16575"
     CDS             complement(2724..>3072)
                     /locus_tag="PLG78_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24789.1"
                     /translation="FARQDANLRSGKQTVDLLFYGKVFHDRDVPGPYRLVGLRGSLNT
                     DVIQPEDLARSPQEVERFLSQIRSDRPMRMVIPYYDKEYKTARYSLDVFTDREYDSPG
                     KQQRIADLSALRR"
BASE COUNT          863 a          790 c          728 g          691 t
ORIGIN      
        1 cgattgcttg cgaaacaatg cccgccttct gacaaattcg cgctgccacc gggaaaaaat
       61 caagatctgc tggatcgtcc tcaagaaggc tcagagcggt cactgctccc tctttccagt
      121 tcttcacctt gatttgatct tgcagggtga tcttttttgc acgtttggac attcgctctc
      181 gcaattggag aatggaaaca catgcgagcg aaagatcatc tatgatttcg ccggtgcttc
      241 cgagcatctc gcgaatccga ggtagatcac cctgagcgga ctggactacg ctacagaata
      301 attctgaatc ttcgttcatt ctggtggtgc catcttctgc gggcaaaatc aaatcgtcgc
      361 gaccgtccga tccaaggatg atgcgatcac cgactctgag ttggattgtt tcaatgaacg
      421 cggcaacatc cactccagga gttcccagct tgttcagtgc atgagggtat tccagaaacg
      481 atgcctgtcc cgcgcggagt agaacaggaa gaggatgttc tgctgcgaga taataggcga
      541 gcccactttg ttcatcgatc aggaacaaga ccagagaaac aagcatactc gcgttgaagc
      601 tgcgataaac agagtccatt tccaggaagg tctgatggag ccatctttca ggtgtgtaat
      661 cagagatagc ggcaccgctc tttgtgcgat tcagaatcgc gtgtattaga cttccgagta
      721 caagaatccc tccacctccc tggatggatt ttcccatagc atccgcattc aaaaccatag
      781 tgtatggttt tccgcgaaga actatgcgat ccgcgatgtt catatctccg ccaatttcag
      841 atttccattt cttgaattcg aatttctttt tctgttctat cagaaactcc accgcaatcc
      901 tctcgctcgc tccatcgttg cgcgcgagag gatcaatcaa catagaagtg agaaaatagt
      961 ccccatcctg tttgcgtttt agatcatcga gattgtcata cgcgcgctgc agctccctgg
     1021 ttcgatcctg cactttgatt tcaagagtgt ccgcgtaatc tttgagtctg gcctgagcgt
     1081 ctcgaatgga tttcaccatt gcattgaagg cccgtgctag aaatccgatt tcatcctgca
     1141 cctgaacggg aacctcatgt tccatatcgc ccttctctac ccgggcgact cccgccagaa
     1201 gtaaattcaa aggcgcgatc aagttcacct gaaacaaaag aggaaacaca gtaagaatca
     1261 cgagtgtcac ggcaaacacg atcacgacgt tctcagtcac cgtcttatgc atctcggaac
     1321 gatagtcgct atacaagaat ccaaactcgt agaggccttg cttcccctgg tacacataga
     1381 aaagataggg gacacctgcc gccgttctat aaagacgatg atcaagttcc acaatctggc
     1441 gatccatttc attggaggca agttcgcgag ccgcagaaat agatgttcct ttttctctct
     1501 tcagctggtt ggctctgcgg aggagttcgt actcgcgcgc ctccacatcc gaatccgcca
     1561 tggcggagga ggacaaagaa gactttgcaa aaataatgcg ataatcgcgc gaatacctgg
     1621 aaggatgagg tctataggcg acgtactgaa gatctcccgg aatgcgcgta tccgcaaaac
     1681 tcctctcccc cagatcaaga gcaaggccag gaagcgcact cttccgccct ccgtcaaaag
     1741 cactctctgt tctctggttg gctctggatc caatcaatcc tgtcacaaca agcaaggtca
     1801 cgagagcaat gccaaccagc ctgaccatca tcgacgtgct ttcacgtgaa tggttgatgt
     1861 agaccattcc gtaccagaaa tacacgaaga gcagcccggt attcagaagc agcgccacga
     1921 gctccttaga aacaaaggat gtcagaaaac ctaccagtac aatgatgttg atcgtgagtt
     1981 ccgcaagcac caggaggaag aaggatcgaa cagcctgagt ttgtgctgat cccgctcgaa
     2041 acaacttact caagacaatt actggacgaa gcaagacacg cagcggccat ttgaaccacg
     2101 gagaaagaga cgaaaactca aaccatggag gcgacggaat caaatggtct atttgtctta
     2161 gtaaaacgat cgccacccag aagaacatga tcatgcttcc gagcgggata taagtagaag
     2221 cgtatcgcga agcgaaaata tgctggtcaa agatcaatct atagccagcg gactccgcgg
     2281 acagcgagta ttcaacgata gccgctattg aggcaagcat cgtaacaaca aaaaccacaa
     2341 tggattcgcg gcgatagatc cagatcggca tgcgataagc aaactgaatc ttgaagacca
     2401 gtgcgatggt gatgaagcac gccaggtgaa acccgtaggg agcggccgca tgaatgccgg
     2461 aatatcccag caaataacca gcattaaaga cagtcaaaaa aaggaaaaac gcacccagat
     2521 agcgcgcagc agtctgacgg cgagaaagca gaaacgcggc aagtataaag gtaagtacgg
     2581 aaggagtgaa gcgatcaaag aagttcgcac tcagctcaag cacttcaaaa tgcatcgttt
     2641 gaatgagttc gcatacaatg cacgtcaatc atatctggag cacaaccggc accttcgttt
     2701 caaaaaaaga tgccggtccc gactcagcga cgcagcgcag atagatctgc aattctctgc
     2761 tgcttcccgg gcgaatcgta ttcgcggtcc gtaaatacgt cgagcgaata gcgggctgtt
     2821 ttatattctt tgtcgtaata aggaatgacc atccgcatag ggcgatcgct gcgaatctgg
     2881 ctcaagaatc gttctacttc ttgaggagat cgggctagat cctccggctg aatcacgtct
     2941 gtattcagcg atccacgcaa accaaccagt cgatagggcc ctggaacatc gcggtcgtga
     3001 aacacctttc cataaaaaag caaatccaca gtttgttttc cagaacgaag attggcgtcc
     3061 tgacgcgcga at
//
LOCUS       DAOQJL010000480         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21441_length_3057_cov_3.876416, whole genome shotgun sequence.
ACCESSION   DAOQJL010000480 DAOQJL010000000
VERSION     DAOQJL010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_3057_cov_3.876416"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1037)
                     /locus_tag="PLG78_16580"
     CDS             complement(126..1037)
                     /locus_tag="PLG78_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE24790.1"
                     /translation="MREENFLGTSPIQSWYLIASSRELRKNQILGAQLGRHKVVLFRN
                     EHGIAGATDAYCPHLGADLSDGCITQGKIRCAFHHWTFRVDGKCETGERNATSYPVME
                     KHGAIWLFNGPSPLFGIPEMDQMHATILKPRVIRAHPHLLTPNGLDIEHFSSVHGIHF
                     SKQPVFKTEPFGIAVDMEIDLNASKSFRMARLFAGNQIRAKFYTLGGHISVIRANAGA
                     VPLHVMFTHRPQPDGTSISRTLLFFPEKMKAPSTLYRFLSGLFLYFIVRDDVRLLEKV
                     QFRSENLKTGEGLALFRDQINQMETMA"
     gene            complement(1030..1926)
                     /locus_tag="PLG78_16585"
     CDS             complement(1030..1926)
                     /locus_tag="PLG78_16585"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE24791.1"
                     /translation="MRIETNIEPPGTLNLFLSSVIVAGWMGGQIIASSISGWTLVAFG
                     VAFSFLTLPLYSLMHECEHNIFHKNKWINDIAGTILSAFFPGPFSFLRGCHLGHHRRN
                     RTEAEMFEIIQPGDSAWKKTGFFYFMYLGGFWLSVPVSMIPLLLWPALLKSRMIQNTV
                     SASAMVNGVPRHFYLRIRLECVFVLVVHLALIWSLNLNPSTYLLILVAGGINWSSQQY
                     IQHAGSPLDVIHGAHNLKASRPYQKWILNFNWHLAHHQHPQVPWNMLPKFHDPSRDRP
                     PYLVSFLKFWKGPRRLEIRRDA"
     gene            complement(1910..2578)
                     /locus_tag="PLG78_16590"
     CDS             complement(1910..2578)
                     /locus_tag="PLG78_16590"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE24792.1"
                     /translation="MQIATATTEQKGMTRCPGRLESTVLENWNHSFIGRVATTVVLIG
                     ITMPLYMWINHSLSPRFDLSISLDETIPFLPWTIFLYLSFYLMLLVAAGVCAAPEYGR
                     IQKSVLLANFLCYAGFFFFPSHYPRPLPMDAGGLRDFYGWLYANDAPGNTFPSIHAAA
                     SLCTGFSMRKIGWKIWGAGIAISILTVKQHFIVDLIGGAAVAALSIWIVRLYEGRRFH
                     ENRN"
     gene            2556..2984
                     /locus_tag="PLG78_16595"
     CDS             2556..2984
                     /locus_tag="PLG78_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE24793.1"
                     /translation="MVAVAICILISEPGFLCQLRDDVPGIRCPGQWGLFGGHIEEGED
                     ARTAVERELMEEICLDAKSFEYLGEFPDDGVIRHVFVHTVETLSGLILKEGWDMAVLT
                     REQLESGFAFSEKAGMIRPVAEPVRLIFRQFFQTRAQKTG"
BASE COUNT          815 a          714 c          797 g          731 t
ORIGIN      
        1 agtgaatcca agcacagtga ttccaagagt caggtaggct gagagcagac agaatacggc
       61 ccagcgagga tcgcgcagtc cgggccacgc tggtttttcc caggtggcgg gattcaagta
      121 aagattcatg ccatcgtttc catctgattg atttgatcgc ggaacagagc gaggccttcg
      181 cctgttttca ggttttctga tctgaattga accttttcta gaaggcgcac atcgtcgcgc
      241 acaatgaaat acaagaaaag ccccgacaag aaacgataga gagtagaagg tgctttcatt
      301 ttttcgggaa agaagagcag cgttctgctg atgcttgtgc cgtccggctg tggtctgtga
      361 gtaaacatca catggagagg tacggctccc gcattggcgc gaatcacaga gatatgaccg
      421 cccagagtgt agaatttcgc tctgatttga ttgcctgcga agagcctggc catgcgaaag
      481 gattttgaag cgttcagatc aatttccata tccaccgcaa ttccaaatgg ttctgtcttg
      541 aaaaccggtt gtttggaaaa atgaatgccg tgaactgaag agaaatgttc aatgtcgagt
      601 ccattcggcg taagcaggtg agggtgagcg cgtatgacgc gcggcttcaa aattgtagca
      661 tgcatctgat ccatttctgg aatgccgaat aacggtgaag gtccattgaa caaccagatc
      721 gctccgtgct tttccatcac cggataagaa gttgcgtttc gttctcctgt ttcgcatttt
      781 ccatccactc gaaatgtcca atgatgaaag gcgcagcgta tctttccttg cgtgatgcaa
      841 ccgtcagata gatccgctcc gagatgagga caatatgcat ccgttgcccc cgcgattcca
      901 tgttcgtttc taaatagaac gactttgtga cgtcccagct gagctcccag aatctggttc
      961 ttccgcagtt ctcgcgaaga ggcgatcaaa taccagctct gaatcgggct ggtgcccaga
     1021 aagttttctt cacgcatcgc gacgaatctc cagtcttctg gggcctttcc agaattttag
     1081 aaaagagacc aaatagggcg gtcgatcgcg agaaggatca tgaaacttcg gtagcatgtt
     1141 ccacggaacc tgagggtgct ggtgatgcgc caggtgccag ttaaaattca ggatccattt
     1201 ttgatacggg cgagatgctt tcaagttgtg cgcgccatgg atcacatcaa gaggagagcc
     1261 tgcgtgctga atgtactgct ggctggacca gttgattcct cctgcaacca gaatcaacag
     1321 gtacgtcgag ggatttagat tcaaactcca gatcaaggcc agatgaacaa ccaacacaaa
     1381 aacacactcg aggcgaatgc gaagatagaa gtgcctggga actccgttta ccatcgcaga
     1441 tgcgcttact gtattttgaa tcattctgct tttcagaagc gcgggccaga gcagaagcgg
     1501 gatcatagat acaggaacgg aaagccagaa accacccaga tacataaaat agaagaaacc
     1561 agttttcttc caagctgagt caccaggttg aatgatttca aacatctccg cttctgtacg
     1621 gttcctcctg tgatggccaa ggtgacagcc gcgcagaaaa gaaaacggac cagggaagaa
     1681 agcggataga atcgttccgg cgatgtcatt gatccatttg tttttgtgga agatattgtg
     1741 ctcgcattca tgcatgagag aatacagagg tagagtgagg aatgagaaag cgacgccaaa
     1801 agcgactaac gtccatccgg aaatggaaga cgcaattatc tgcccgccca tccagccagc
     1861 gacaatcacc gaggatagaa ataggttcag cgtgcctggt ggttcgatgt tagtttctat
     1921 tctcatggaa tcgccggcct tcgtacaatc gcacaatcca gatgctcagg gcggccaccg
     1981 ccgcaccgcc aattagatcc acgatgaaat gctgcttcac tgtcaaaata gatatagcga
     2041 ttccagctcc ccagatcttc cagcctattt tccgcatgga aaaaccggtg caaagggatg
     2101 cggccgcgtg aatgctcgga aatgtgtttc caggagcatc gtttgcgtaa agccagccgt
     2161 agaaatctct aaggccccct gcgtccatgg gtaagggtct tggataatga gacggaaaaa
     2221 aaaagaatcc tgcgtagcag aggaagttcg ccagcagaac acttttctgg attcgaccat
     2281 actcaggcgc ggcgcagact cctgccgcaa caagtagcat aagataaaag ctgagataca
     2341 aaaaaatagt ccacggtaga aacggaatcg tctcatccag agaaatgctc aggtcaaatc
     2401 tcggagacag agaatggttg atccacatat agagaggcat tgtaatgcca atcagcacca
     2461 ctgtggtggc aacgcggccg ataaacgaat gattccagtt ttcgagcaca gtcgattcta
     2521 gtcgacccgg acacctggtc attccttttt gttctgtggt tgcagtcgcg atttgcattc
     2581 ttatttccga gccaggattc ctttgccagt tgcgcgatga cgtgcctgga attcgctgtc
     2641 caggtcaatg gggtcttttc ggaggacaca tcgaagaagg cgaagatgct cgtactgcag
     2701 ttgagcgtga attgatggaa gaaatttgcc tggatgcgaa gtcgtttgaa tatctgggag
     2761 aatttccgga cgatggagtg attcgtcatg tattcgttca tactgttgaa actctctccg
     2821 gcttgattct aaaagaaggt tgggacatgg cagtgctcac gcgggagcaa ctagagtcag
     2881 gtttcgcgtt cagcgaaaag gcgggcatga ttcgcccggt tgcagagcct gttcgcctga
     2941 tctttcgtca gttttttcaa accagggctc agaaaaccgg ttgatgacaa gcggcgcaga
     3001 tggagaatct gcgcatgcgt ttttcctttc agtacaccgc cattacctgc gatgcgg
//
LOCUS       DAOQJL010000481         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21458_length_3055_cov_3.900000, whole genome shotgun sequence.
ACCESSION   DAOQJL010000481 DAOQJL010000000
VERSION     DAOQJL010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21458_length_3055_cov_3.900000"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3014)
                     /locus_tag="PLG78_16600"
     CDS             complement(<1..3014)
                     /locus_tag="PLG78_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE24794.1"
                     /translation="MKLLAKSPDDRYQSAEGLLYDLNQCRAALRTEGVIPDFVIGGRD
                     FSSTLHLPDDLFGREDQKQTLQRLVRSAVSGQSSIALVMGEPGIGKSALVASCLTDVF
                     SARGVVLNGRFDSLGRDRPYHGIRQAIQSVVNQMMGESEELLSETRTRLVADVAEYAS
                     VLVSAFQELSEILQSEGSGNMSGAEFDRQLEEGFVRLLTSLAGGGPVVLALDDVQWAD
                     HASRRLISRMLSRRNMGSLLCLLSVREQEIDPLHPVLQIMKEQDNEHTVVIRLGPLSE
                     EDIFEILTRTLRTEGAPVRQLATLLRAKTEGNPFFLRQYLRDLGNDGTLKAIDGKWVW
                     DYSAIQSRSIADNVIDLVVTKILYEKGEAQSLLKLISCLGSRAPVDLLAISTGRTSAD
                     LEAILEPLVRNGFLVLEGRNVSFVHDRVREAAHTLMTDDERSSNHLVLGRALLRDHTI
                     IGDAEYTIPAVLNPVMHLLNPAERLELARLNLAAGSRARSGGAFSEAGRYFSQGYSLI
                     EDANWDTDYELSFALALGLAESSYLNEEHAKAVDLFQVCSGRARDPLDSARVQELRAL
                     LLLSEGKLSEAIDAGLTGLDTLGIRLPRHPGQMGPLPDLIACRLWFVRRKIEDLSYLK
                     DASDSRIRAAMHLMMIIGNAAFIGRPALVPVVIARMVRISLRYGFFEETTFAIVMFGL
                     IIYALGDPSSAIRFGATGAAMLSRVKSKEITGKVLYIHACFIRHLSEHGGKSIEAFLE
                     GYSYSVTAGDTQFQSYNANHYCIQHLLYGGHFGEALEQFSRFTTLLEQAGNFDPIQYH
                     KAFKQFAKYLTTGGQNVSTSLEGADFDVATAERKLKESGSFTVLFPIYMLQGVLELLS
                     GNYAGAIERFTEMRKFESGALGMPFVPHSCMFESLAALDYASLHSADRDALIRRARKL
                     LKRLKTWSKLGPENYLHKALLVEGAIAEARDEIHRAFELYDHAIERSLEGGFVFEAAL
                     ASERAGKMYRRIGRERLSRIYASSAHDLFQRCG"
BASE COUNT          790 a          859 c          723 g          683 t
ORIGIN      
        1 ccacatctct gaaacagatc atgagcggag cttgcgtaaa tccgcgagag gcgctctcgc
       61 ccaattcgtc tgtacatctt gccggcacgt tcagatgcca gggccgcctc gaacacaaac
      121 ccgccctcca agctgcgttc aatcgcatga tcgtacaact cgaaggctcg gtgaatctca
      181 tcgcgagctt ccgcgatcgc accttctaca aggagagctt tgtgcagata gttttccggc
      241 cccagctttg accatgtctt cagcctcttg agcaactttc gggctcttct tatcagcgca
      301 tcacgatccg cgctatggag cgatgcgtaa tccagtgctg ccagagactc gaacatacag
      361 gagtgaggaa caaatggcat ccccagagca ccagactcaa acttgcgcat ctcagtgaag
      421 cgctcgatcg cgcccgcata attgcccgac aaaagttcca gaacgccctg caacatgtag
      481 atcgggaaca acacagtgaa gctaccggac tctttcagtt ttcgctctgc tgtcgcgacg
      541 tcgaaatccg cgccttcgag agacgtggac acattctgtc cgccggtcgt cagatacttc
      601 gcaaattgtt tgaatgcctt gtgatattga atcgggtcaa aattcccggc ttgctccagc
      661 aatgtagtga agcgactgaa ctgttccagt gcctctccaa aatgaccacc atagagaagg
      721 tgttgtatgc aataatgatt tgcattgtaa gattgaaact gcgtatcgcc tgcggtcact
      781 gagtacgaat agccttctag aaacgcttca atgctcttgc cgccatgctc agagagatgc
      841 cgaataaaac acgcgtgtat atagagaacc ttcccggtta tttccttact cttgactcga
      901 gagagcatgg cagcccccgt tgctccgaat cgaatcgccg aagatggatc ccccagggca
      961 taaatgatca gaccgaacat tacgattgca aaagtagttt cttcaaagaa accataacga
     1021 agagagattc gcaccatgcg cgcaatcact acaggcacaa gcgcaggtct gccaatgaat
     1081 gccgcatttc cgatgatcat catgagatgc atcgcggctc gaatccgcga atcgctagcg
     1141 tccttcagat aagacagatc ctcgattttt ctgcgcacaa accacaagcg acaggcaatc
     1201 aaatcaggaa gaggacccat ctgtcccggg tgccgcggca ggcgaatccc gagagtatcg
     1261 agtccagtga ggccagcgtc gatcgcttcg ctgagttttc cctcactcaa taggagcaga
     1321 gcgcgcaact cctgaacgcg agcagagtca agtggatcac gcgctcgccc tgaacaaacc
     1381 tggaataaat ccacagcttt cgcgtgctct tcattcaagt aagagctttc tgcaagtccg
     1441 agagcaagag caaacgaaag ctcgtagtca gtatcccagt tcgcatcctc aatcaaagaa
     1501 tatccctgcg aaaagtacct acctgcctca gaaaaagccc caccacttct tgcgcgagaa
     1561 cctgccgcaa ggttgagcct tgcaagttcc agtcgttccg caggattcaa caaatgcatc
     1621 actggattca ggactgccgg gattgtgtac tcagcatcac caatgatggt gtggtctcgt
     1681 agcagcgcgc gccccaggac aagatgattg gaagacctct catcatccgt catgagagta
     1741 tgagctgcct ctctcacgcg atcgtgtacg aacgaaacgt tgcgaccttc cagaaccaga
     1801 aacccgtttc gaacgagcgg ttccagaata gcctcaagat ccgcagaagt tcgtcctgta
     1861 gaaatcgcaa gtagatcaac aggagctcta gatccaaggc aggaaatgag tttgagcaaa
     1921 gattgggctt cgcctttctc atacaggatt tttgtaacga ccagatcaat aacgttatcc
     1981 gctatactgc gcgattgaat cgccgagtag tcccagaccc attttccatc tattgccttc
     2041 agtgtgccgt cattgcccag atcacgcaga tactgacgta agaaaaacgg gtttccttct
     2101 gtttttgcgc gtaagagcgt agccagctgt ctaaccggcg caccctccgt tctcaaagtc
     2161 cttgttaaaa tttcgaaaat atcttcttcc gaaagaggcc ccaggcgaat gacaactgta
     2221 tgctcgttgt cttgttcttt catgatttga agcacaggat gcagaggatc aatctcctgc
     2281 tctcgcacag acaacaaaca aaggagcgat cccatgttgc gacgcgagag cattctagaa
     2341 atcaatcgac gactggcgtg atccgcccac tgaacatcat cgagcgcaag aacaactggc
     2401 ccgcctccgg ccagactcgt cagaagacga acaaaaccct cttcgagttg ccgatcgaat
     2461 tcggctccgg acatgttccc ggatccttca gattgcagta tttcagaaag ttcctggaaa
     2521 gctgaaacca ggacactggc gtattccgcg acatctgcca ccagtcgcgt tcgcgtttca
     2581 gacagaagct cttcgctttc ccccatcatc tgatttacga ctgattgtat agcctgtcgg
     2641 atgccgtggt agggtcgatc tcgacccagg ctgtcaaatc ggccgttcaa gacaactccg
     2701 cgggcggaga atacatccgt caggcaagag gctacgagcg cggacttccc aattccaggc
     2761 tctcccatca ccagagcaat cgagctttga ccactcaccg cagaacgcac gaggcgttgc
     2821 aaggtttgct tttgatcctc gcgaccgaag agatcatcgg gcaaatgaag agtggagctg
     2881 aaatctctgc cgcctattac aaagtccggg atgacccctt ctgtgcgcaa agccgcgcgg
     2941 cattgattca aatcgtagag cagtccttcc gcactctggt agcggtcgtc cggggacttg
     3001 gcaaggagct tcatcacaat atcgcccatc acttttggaa tcgatccaac gagtt
//
LOCUS       DAOQJL010000482         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21641_length_3040_cov_4.721273, whole genome shotgun sequence.
ACCESSION   DAOQJL010000482 DAOQJL010000000
VERSION     DAOQJL010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21641_length_3040_cov_4.721273"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>3040
                     /locus_tag="PLG78_16605"
     CDS             42..>3040
                     /locus_tag="PLG78_16605"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24795.1"
                     /translation="MRIFVLCIGLTFAGPLTAQHVVQNVKSLTQVESLDSTEQTPHRW
                     WVTEQPVDPVAITRQVLESKNENPGSAWKAIPVPSNVRDYYPHMTRKQMSVTYLKAVV
                     LPDEPAAATALMMGIINDRDRVYVNGTFIGATGIWDSPVATAYDKRRIYQIPAGVIRP
                     GELNVIVAQVQGLSVDLWGMDQYRTAIGPAMTIFSSEINESFRALLFLTCYTTVGLYF
                     FFMWARRRQDKQNLFFAVFCVLLIAYNLLRSQLRYDRGISFLELKRLEYIALYAVVPF
                     FFLFFRSYFHLPKTKVVKVFDGLMTLVCVVLLAGIGHTLVTNDTDGWWKFQRTIVQLG
                     CWPPLVVGSVGILVYRMFKKDKDALAMFISMILLVAAMVLDIASSMGKVNFPAIFQYV
                     FIAFILSIGTVLSNRFLRLHREIEDLNANLEQKIETRTRELNQTVAKARAIKEQQDGD
                     YFLTSLLIEPLSGVFSTGGPVHAESFVRQKKEFEFRKWKSDLGGDLAIVDRIGLRDKD
                     FTVFINADAMGKSMQGAGGAVVLGTTFQSLISRTKRGINASRHPEQWLRDAFFDLQNV
                     FVAFDGSMLVSAIFGLIDEASGTLYYINSEHPGAVLYRDKKAQFLTDAGLIRKFGIEG
                     IEENLHVNMLRLKPDDVVILGSDGRDDLHIGEDPQGGRIINDDESLFLRIVEEAQGNL
                     NEIVEGLTTRGQIIDDLSLIRLGYREDAPAIQTAVLPGGYESLLSAGKAAFARKDSAE
                     ARTRLEGALKLHESEEVYEMLVRCCLAQKDFRAAADLVDQATLLYPANHRLLYLALRA
                     FKGTGQYDRAVEYGERAALRDPDNTDNLMHLADCYRLLKKSSRAQDAIRRAEKKQASP
                     DLMRKLKGLLGVAVLAIALTPSLRAESPIQLETSRDQQWQITSDEIDPEAETRAFFSE
                     ARTSDQTVVWLPVQVPGERRGAKPGTIWLRTTLNLKEKPTSSLAFRAGIIRDRDRVFI
                     NGNLIGSTGEWDASAPQAFDRSRLYEV"
BASE COUNT          780 a          783 c          769 g          708 t
ORIGIN      
        1 aaaaacgctt tgcgaacatc ctcgacgggc catattccgc cgtgcgcatc ttcgttctgt
       61 gcatcgggct cactttcgcc ggaccactca cggcccagca cgtagtccag aatgtaaaat
      121 cactcaccca ggttgaatcc ctggattcaa ccgagcaaac gccccatcgg tggtgggtca
      181 cagagcagcc cgtggatcca gttgccatca ctcggcaggt gcttgaatcc aaaaacgaaa
      241 accccggctc tgcctggaag gcaatcccgg ttccatctaa cgttcgcgac tactatccgc
      301 atatgacgcg aaagcaaatg agtgtgacct acctcaaggc ggttgtgcta ccggatgagc
      361 cagcagcagc cacggcactg atgatgggaa tcatcaacga cagagacaga gtatacgtga
      421 acggaacgtt catcggtgct acaggaatct gggattcccc tgtggccaca gcttacgaca
      481 aacggcgcat ctatcagatc ccggcaggag tcattcgacc aggagagctc aacgtaattg
      541 tagctcaggt gcagggtctg tctgtggatc tctggggaat ggatcagtac aggacggcca
      601 tcggtcctgc catgaccatc ttttcatctg aaatcaacga atcttttcgc gcgctccttt
      661 ttctaacctg ctacacaacc gttggacttt atttcttttt catgtgggcc cgtcgcagac
      721 aggacaaaca gaatttgttt ttcgcggtct tttgcgtctt actcatcgcg tacaatttgc
      781 ttcgcagtca gttgagatat gatcgaggga tcagttttct cgaattgaaa agactcgaat
      841 acattgctct ctacgcagtt gtgccatttt tctttctctt ctttcgctct tactttcatc
      901 ttcccaaaac taaagtcgtg aaggtcttcg atggtctgat gaccctcgtc tgtgtggtgt
      961 tactcgctgg aattggtcat acactcgtta cgaatgacac ggatgggtgg tggaaatttc
     1021 aaaggacaat cgtacaactt ggatgctggc ctcccctggt ggttggctcc gtcggaatcc
     1081 ttgtctatcg tatgttcaag aaggataagg acgcattggc catgttcatc agcatgatcc
     1141 tgcttgtggc cgcgatggtt ctcgacattg cgtcgagcat ggggaaagtc aattttcctg
     1201 ctatttttca gtacgtcttc atcgctttca tcttgagcat aggaaccgta ctctccaatc
     1261 gattcctgcg ccttcaccgc gaaatagaag acttaaatgc gaatctggaa cagaagatag
     1321 aaacgcgaac cagagaattg aatcaaacgg tggcaaaggc acgggcaatc aaagaacagc
     1381 aggacggaga ttacttcctt acttcccttc tcattgaacc gctttccgga gttttctcca
     1441 caggcgggcc ggttcatgcg gagagttttg ttcgtcagaa aaaagaattc gaattcagaa
     1501 aatggaaatc ggatctgggc ggagaccttg caatcgtaga tcgaatcggt ctgcgcgaca
     1561 aagacttcac cgtatttatc aatgcagacg cgatgggaaa atcgatgcag ggcgccggcg
     1621 gtgcggtggt actgggaacc acgtttcaat ccctcatttc tcgcacaaaa agaggaatca
     1681 acgcaagcag acatccggaa cagtggctgc gcgatgcgtt ctttgatctg caaaacgtgt
     1741 tcgtagcgtt tgacggaagc atgcttgtgt ccgccatctt tggcctcata gacgaagcat
     1801 ctggaactct gtactacatc aactcggaac atcccggcgc tgttctttat cgagacaaga
     1861 aagcgcaatt cctgacggac gcgggtttga tccgcaaatt cggaatcgaa ggaattgaag
     1921 agaatctaca cgtcaacatg cttcgcctga agccggacga tgtggtcata ctcggaagtg
     1981 acggacgcga tgatcttcac ataggagaag acccgcaggg cggcagaatc atcaatgacg
     2041 atgaatccct ttttctcaga attgtggaag aagcacaggg aaatctaaac gaaatcgtag
     2101 aaggactgac gactcgcggt caaatcatag atgacctgag cctcattcgc ctgggataca
     2161 gagaagacgc gcccgccata cagaccgccg ttcttcccgg cggatacgaa tctcttcttt
     2221 ctgcaggcaa agctgctttc gcgcgaaagg attcagcaga agcacggacc agactggaag
     2281 gcgcactcaa acttcacgaa agcgaagaag tctatgaaat gcttgtgcga tgctgcctcg
     2341 cacagaagga ttttcgcgcg gcggcagacc tggtagatca ggcaactcta ctctatcctg
     2401 ccaatcacag gcttttgtat ctggctctcc gtgcattcaa gggcacgggt cagtacgacc
     2461 gggccgttga atacggagaa cgcgctgcgc tgagagatcc ggacaatacg gacaacttga
     2521 tgcatctggc ggattgctac agactactca agaaatcatc tcgcgcccag gatgcgattc
     2581 gccgcgcaga aaaaaagcag gcttccccgg atcttatgcg aaaactcaaa ggtttgctgg
     2641 gggtcgcagt gctggcaatt gctcttacgc catccttgcg cgcagaatct cccatccagc
     2701 tagaaacatc acgcgaccaa caatggcaaa taacgtcaga cgagattgat ccggaagcag
     2761 aaaccagagc ctttttctca gaagcgcgca cgagtgacca gactgtagtc tggcttccgg
     2821 tgcaggtgcc cggagaaaga agaggcgcaa aacctggcac catctggctt cgcacgactc
     2881 tcaatttgaa ggaaaaaccc acatcgtctc tagcctttcg cgctggaatc attcgcgacc
     2941 gggatcgcgt gtttatcaac ggcaacctca ttggtagtac aggagaatgg gatgcaagcg
     3001 ctccccaggc atttgacaga agtcgtctct acgaagtgaa
//
LOCUS       DAOQJL010000483         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21718_length_3034_cov_3.544814, whole genome shotgun sequence.
ACCESSION   DAOQJL010000483 DAOQJL010000000
VERSION     DAOQJL010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21718_length_3034_cov_3.544814"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..489)
                     /locus_tag="PLG78_16610"
     CDS             complement(163..489)
                     /locus_tag="PLG78_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24796.1"
                     /translation="MKRGGIFQLLLVLAVFLVPFGVVHFYTRNIVSASLLRRDIHLAG
                     LKKEQLVKKNMALKEALGRLTGRDLVLPSEGLPFSENNRIVRLRMSRDSEAAETRSVP
                     DQPALN"
     gene            complement(486..1349)
                     /gene="rsmH"
                     /locus_tag="PLG78_16615"
     CDS             complement(486..1349)
                     /gene="rsmH"
                     /locus_tag="PLG78_16615"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNE24797.1"
                     /translation="MEFVHVSVMPEEVISWAPQDARLIVDGTAGEAGHTLLLARRCPN
                     ARILAFDRDPEMLERARKKLGLAGMENRVELIRGSFGEIPEHTREADFILLDLGVSMH
                     HFRGAGRGFSYTDDSLDMRLDPDLEKTAADIINHSSEKDLKLIFQNYGEERFAGRIAR
                     AIVQKRPVRTARDLSSIILGSVPRSRSHPATRVFQALRIAVNDELGEIERALPLLARS
                     LAPGGRLAVISFHSLEDRIVKHGLRALDLKILTKKPLEPTEEEVRANPASRSARLRVA
                     EKPADNAGEGP"
     gene            1400..2365
                     /locus_tag="PLG78_16620"
     CDS             1400..2365
                     /locus_tag="PLG78_16620"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE24798.1"
                     /translation="MKKGTLVFNPNSGKGKAKERATNFASVWKNEFGNDLILRPTRSL
                     ADIRVAALETIQDPEMVPIFMGGDGTLSESVQGIAEHQNFQPIDRPVGLLPGGTGNSF
                     LRDFGVTNYEEARDNLIGALRSKSVQNADLAIIKYQAYKPERPDQPGSSVRRITFNIF
                     GVGIIADITEMAVKMRWMGAANYTVASLWKIFSHNLYNFEITIDGKREDMACNMISVH
                     NSQYTGGAMHIAPSVRVNDGQLFYLVPRYVGKLTLLKKFPELFKGTHVNSDQMRTGFV
                     KNIGIRHAHPMWMNVDGELERGWNPDLSIQPNFWKIYMMAERMLP"
     gene            complement(2384..2815)
                     /locus_tag="PLG78_16625"
     CDS             complement(2384..2815)
                     /locus_tag="PLG78_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24799.1"
                     /translation="MPTGWVRINGYRGAIDDPLLRSYINFPLNPFVRVDAPNLLDYAF
                     LVRNKQTFRASGTGGVDLKVHTAKGPFTPYLDIGLFNRGGSASLGARYVFGAGVYFTA
                     EGFTEEIATFVPARDLRGKSSRYIDNNGCRFGVGLVVDSGR"
     gene            2808..>3034
                     /locus_tag="PLG78_16630"
     CDS             2808..>3034
                     /locus_tag="PLG78_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE24800.1"
                     /translation="MGIKIAGPDLGVIQQVGNEIEAALKSVPGTASAFAERVSGGRYI
                     EVVPDRLAAARYGLTIEALQRVISVAVGGEN"
BASE COUNT          750 a          810 c          817 g          657 t
ORIGIN      
        1 cccgccgatc ttgcagctgc aaggtatgtg gatttaggcg ttccaaaaaa aaggacacca
       61 ggacgggcat cgcgcgaatc atccgtaata cgtatgattt ctatttgagc atatccggga
      121 ggacagacgc actctccgga gcagatatcg atcagtttca cttcagttca atgcgggctg
      181 gtctggcact gagcgtgttt ctgcggcctc agaatcgcga gacatgcgaa gccgcacaat
      241 tctattgttc tcagagaacg gaaggccttc tgagggcaaa accaggtccc tgccggtgag
      301 tctccccagg gcttccttca gcgccatgtt ttttttaaca agctgctctt tcttcagtcc
      361 ggccagatga atgtcacggc gaagcaggga ggcagatacg atgttgcggg tatagaaatg
      421 aacgaccccg aacggaacca gaaacacagc cagaacaagc aaaagctgaa aaataccgcc
      481 gcgtttcatg gaccctcgcc tgcattatcg gcaggtttct ctgccactct gagccgcgca
      541 gacctggaag cagggtttgc acgcacctcc tcttccgtgg gctcaagagg cttctttgtc
      601 aggattttga ggtcaagggc acgcaggcca tgcttgacga ttctgtcctc aagagaatga
      661 aaggatatca cagccagcct tccgccaggg gcaaggctac gggcgagaag aggaagggct
      721 ctttcgatct ctcccagttc atcgttcacc gcaattcgaa gggcctggaa gactcttgtg
      781 gccggatgcg acctggacct gggaacgctg cccagtatta tagaagaaag atcgcgggcc
      841 gtgcgaacgg gccttttctg aacgatcgcc cttgcaattc gacctgcaaa tctttcttct
      901 ccatagtttt gaaaaatcag cttcaggtcc ttttcactgg agtgattgat gatgtccgcg
      961 gcagtcttct ccagatcagg gtcaaggcgc atgtccagcg aatcgtcggt gtaggaaaag
     1021 cctctgcctg cccctctaaa atgatgcatg gatacaccca gatcgagcaa aatgaaatcc
     1081 gcttctcgcg tatgttccgg gatctcacca aacgaaccac ggatgagctc tacgcggttc
     1141 tccatcccgg ctaacccaag cttcttacgg gctcgctcga gcatctctgg atcacgatcg
     1201 aatgcaagga tgcgggcgtt cggacagcgc ctggcaagaa ggagcgtatg accggcctct
     1261 ccagcggttc cgtctacgat gagacgggca tcctgcgggg cccaggaaat gacttcctct
     1321 ggcatgactg atacgtgtac aaattccatg cgaagatatt agattgacac ggaggtttaa
     1381 ctaagccccc tgattcacca tgaaaaaggg cacactggtc ttcaatccga acagcggcaa
     1441 gggaaaagca aaagaacgtg ccacgaactt cgcctccgtc tggaagaacg aatttggcaa
     1501 tgatttgatt ttgcgcccta ctcgatccct ggccgacatc cgcgtggccg cccttgaaac
     1561 catccaggat cctgaaatgg ttcccatatt catgggcgga gacgggactc tgtccgagag
     1621 tgtgcaggga atcgcggagc accagaactt ccagccaatt gatcgaccgg ttggcctttt
     1681 gcccggtggg actggaaaca gcttcctgcg cgatttcggt gttacgaact acgaggaagc
     1741 gcgagacaat ctgattggag ctcttcgctc aaagtctgtt cagaacgcgg accttgctat
     1801 catcaaatac caggcatata aaccggagag acccgatcaa cccggatcct ctgttcgccg
     1861 cataacgttc aatatcttcg gtgtgggtat cattgcggat attacggaaa tggctgtcaa
     1921 gatgaggtgg atgggagcgg ccaactacac agtagccagt ctctggaaga tcttttcgca
     1981 caatctgtat aactttgaaa tcacgatcga tggcaagaga gaagacatgg catgcaatat
     2041 gatcagcgtg cacaattctc agtacacagg cggggccatg cacatcgcgc cgtctgttcg
     2101 agtcaatgat ggacaacttt tctaccttgt tccgagatac gtgggcaaac tcactctgct
     2161 gaaaaaattt ccagagctat tcaaaggaac ccatgtaaat tcagatcaaa tgcgaacagg
     2221 atttgtaaag aacattggga tcagacatgc ccatccaatg tggatgaacg tggacggaga
     2281 actggaacgc ggttggaatc cggatctttc gattcaacca aacttctgga agatctacat
     2341 gatggcagaa cgcatgctgc cctgagggag ctctcagtgg cgcctaacgg ccggaatcaa
     2401 ccacgagccc aacaccgaac cggcacccat tgttgtcaat gtatcgcgag cttttgccgc
     2461 gcaagtcccg ggcgggaaca aacgtggcaa tttcttccgt gaagccttcg gcggtgaaat
     2521 acacgcccgc tccaaaaacg tagcgcgcac ccagcgatgc gctgccgcct ctgttaaaca
     2581 ggccgatatc aagatagggt gtaaacggac ctttcgcagt atggaccttc aaatcgactc
     2641 cgccagtgcc gctggcgcga aacgtttgtt tgttacgaac taaaaaggcg taatcaagca
     2701 gattgggtgc gtctactctt acaaaaggat tgagagggaa attgatgtat gatctcagca
     2761 ggggatcgtc aatggctccc ctgtatccgt tgattctaac ccagcctgtg ggcatcaaga
     2821 ttgccgggcc ggacctcggg gtcatccagc aggtgggcaa cgagatcgag gcggcgctga
     2881 agagcgtgcc aggcaccgcc tcggcctttg cggagcgcgt ctcgggcgga cgctacatcg
     2941 aggtcgttcc cgaccgcctg gccgccgcgc gctatggcct caccatcgag gcactgcaac
     3001 gcgtgatcag cgtcgccgtg ggcggagaga acat
//
LOCUS       DAOQJL010000484         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21834_length_3025_cov_4.550168, whole genome shotgun sequence.
ACCESSION   DAOQJL010000484 DAOQJL010000000
VERSION     DAOQJL010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21834_length_3025_cov_4.550168"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..638
                     /locus_tag="PLG78_16635"
     CDS             165..638
                     /locus_tag="PLG78_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24801.1"
                     /translation="MNKLMLCICVCLAFASCRHSGDPERESLLAFLSSEYVNSARYPC
                     TVSPLLSTTATALTFRTTSAGTQMAFAVGGAQGGQNLILNSTTTSLNPAPVLPAATTG
                     TGCTTASASSIPAALSTCSSTTTSMSCGVLLSNTYLFRFEFSVASAPTDITAKIQ"
     gene            782..913
                     /locus_tag="PLG78_16640"
     CDS             782..913
                     /locus_tag="PLG78_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24802.1"
                     /translation="MDSVLSYSTIMQITDTEGLALWLLLSVPIACMSLYLAVQLILN"
     gene            1138..1443
                     /locus_tag="PLG78_16645"
     CDS             1138..1443
                     /locus_tag="PLG78_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNE24803.1"
                     /translation="MMIDKIGGISPNQGPRKSEPAYRAETPARASDNVSISEEALRAS
                     DAARVQRLASTPDESRVEKLKEVKEKLARGDYDKLSDEMLGQVADRVAKAFQGSQGS"
     gene            complement(1505..2485)
                     /locus_tag="PLG78_16650"
     CDS             complement(1505..2485)
                     /locus_tag="PLG78_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24804.1"
                     /translation="MRFFLLLLLIGFSSLAAETVYLRDGRVLTGRITSQTRTDITIVT
                     PQGPMNIQKDQIRRIQYDTAAEDKAREDARKAEEARRLQEQQQRQAEEARKLQEQQQR
                     QAEEQKRLEAEKKAQEQKVADQKKAEEEKRKAEEAKRQAEGESTASAQERKRIEEKLR
                     RMREFDERARLMGPTWYGAVARNVAMPGWGEIYQGRKIGYLYAGAWAGLATFALYEKD
                     RYLKAKSVYSSTTRGFLYGTPYVARTFLSYSETDGQASFWFFKGYGQTSAARARMQKH
                     ALRLRFAQGALVALYGWTITDALLFKPTPKTAVGFSSTGDGMRLAVSTEF"
     gene            complement(2503..>3025)
                     /locus_tag="PLG78_16655"
     CDS             complement(2503..>3025)
                     /locus_tag="PLG78_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNE24805.1"
                     /translation="FMLAADQAVVWRGPMLGKAVEQFLYEVIWGDLDVLLIDLPPGTG
                     DVQLSLAQLIDVDGAVIVTTPQNVALQDAARAASMFQQVKIPILGIIENMSEFQCPNC
                     GHITNIFSKNGGSAMAAKMGVPQLGSIPVEKEIMDSGEAGLPITLKDPSGKVAEAYKK
                     IADRILVEVEKFK"
BASE COUNT          700 a          855 c          714 g          756 t
ORIGIN      
        1 ggtccgagtg tgtgcccgtc ctcccctcct ggtcgcctga cggacgcgca cacaccgcgg
       61 actgacgtag atcgcctcaa attgggaccg cgctcacagg gaattctatg tgtttgaatc
      121 ctgcttgaaa agtcagacgg aaattgaaac ctatgatttc catcatgaac aaacttatgc
      181 tatgcatctg cgtttgcctg gccttcgcat cttgcaggca ttcaggggat ccagaaagag
      241 aaagccttct tgcgtttcta tcttctgaat atgtgaattc agcgagatat ccctgcactg
      301 tttctccgct tctttccacg actgccactg cacttacttt cagaacaact tctgcgggaa
      361 cgcaaatggc cttcgcagtg ggcggagctc agggtggaca gaatctaatc ctgaattcaa
      421 ccacaacgag tttgaatccg gcccctgtcc ttcctgccgc caccactgga actggatgca
      481 ccactgccag tgcgtcgagc attccggcag ccctctctac ttgcagcagc acaaccactt
      541 ctatgagctg tggcgtacta ctttccaata cctatctgtt tcgattcgag ttcagcgtcg
      601 cttccgcacc gacggatatc acggcgaaga tccaataaaa aaaatcgcgg ggcgccgtaa
      661 aaaatacgac gcgagctcaa aaaaaacaac tttcagttcc aaaaaaaatg aaaccgctgt
      721 tacgcgaaaa tgcccaattt ccaggcaatc atttttagaa taactcgctt tttctgcttg
      781 gatggattct gtactgtctt attctacgat tatgcagatc acagatacag aaggactggc
      841 actctggctt ctgctgagcg tgccgatcgc gtgcatgagc ttatatcttg cagttcaact
      901 gatactgaac tgaagatccg ctttccacgc atattacagg caaatctcta cctgagagca
      961 gagggatgga tcttttgtct acacgtatac accctccccc catccgtaaa aaaattggcc
     1021 gaattccggg gaaatcgccg tgtacttcct gcacaagtcc cctctccccc ccgggaacgc
     1081 ttttttttct tatgttcatc tgcaatagga ccgataaggt agcatagaga ggtccatatt
     1141 atgattgata aaattggcgg catatcccca aatcagggcc cgagaaaatc ggagccagca
     1201 taccgggcag aaacaccggc cagggcctcg gacaatgtca gcatttccga agaagcactc
     1261 cgtgcatccg acgccgccag agtccagagg ctcgcatcaa ccccggacga atccagagtt
     1321 gagaagctga aggaagtcaa agagaaactc gcccgtggcg actatgacaa actctctgac
     1381 gagatgctag gccaggtcgc agaccgcgtg gcaaaggcat tccagggatc ccagggttct
     1441 tgagttaacc aaaaagtgtt ggggaagtaa gccgcggaac accgcggctt ttttattgcc
     1501 ctgactaaaa ctcggtgctc actgccagcc gcattccgtc tccggtgctg ctgaagccta
     1561 ccgcagtttt gggcgtaggt ttgaagagaa gagcgtctgt gatggtccat ccatacaatg
     1621 caaccagtgc tccctgggca aaacgcaatc tcagggcatg cttttgcatt ctggcacgag
     1681 cggcactggt ctgcccgtag cctttgaaga accagaaaga agcctggccg tccgtctctg
     1741 aatagctgag gaaagtccgc gccacatacg gcgtcccata caggaacccg cgcgtagtag
     1801 aagagtacac tgattttgcc ttaagatagc gatctttttc gtacagagca aatgtggcaa
     1861 gtccggccca ggcacctgca tacagatatc caatctttct tccctgataa atttcgcccc
     1921 agccaggcat ggccacattt cgcgcgaccg ctccgtacca cgttggcccc atcaaacgtg
     1981 cgcgttcatc gaattcgcgc atgcgacgaa gcttttcttc tatgcgtttg cgttcctgcg
     2041 cactggcagt ggattcgccc tctgcctgac gtttggcctc ttccgctttt ctcttctctt
     2101 cctctgcttt cttctgatct gctactttct gctcttgcgc tttcttttcc gcttcgagtc
     2161 ttttctgctc ttccgcctga cgctgctgct gttcttgcag ttttcgtgct tcttctgcct
     2221 ggcgttgctg ctgctcctgc aatctgcgcg cctcttccgc cttgcgtgca tcttcgcggg
     2281 ctttatcttc cgcagctgtg tcgtactgaa tgcgacgaat ctggtccttc tggatattca
     2341 tgggaccctg aggagtcaca atcgtgatat ccgtgcgagt ctgactggtg attcttccag
     2401 tgagaacacg accatctcgc aagtagacgg tttcagcagc cagagatgag aagccgatca
     2461 ggagcaacag gaggaagaat cgcataccca cgatttgacg cattatttga atttctcaac
     2521 ttccacgagt atcctgtctg caattttttt atatgcttcc gcaactttgc cggatggatc
     2581 tttgagagta atcgggagac cagcctcccc ggaatccata atctcctttt ccacagggat
     2641 ggatccgagc tggggcactc ccatcttcgc cgccatcgca gagccgccat tctttgagaa
     2701 aatgttcgtg atatgcccac aattcgggca ctggaactct gacatgtttt caatgatacc
     2761 aaggatcggg attttcacct gctggaacat ggacgcagcc cgggccgcat cttgtagtgc
     2821 gacgttctgc ggagttgtca caatcacagc tccatctacg tcgatgagct gcgccagaga
     2881 aagctgaacg tctccagtac caggcggaag atcgatcaag agcacatcga ggtctcccca
     2941 gatcacctca tagaggaact gctctactgc ttttccaagc atcgggccgc gccagacgac
     3001 tgcctggtcc gcagccagca taaat
//
LOCUS       DAOQJL010000485         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_21850_length_3024_cov_3.759178, whole genome shotgun sequence.
ACCESSION   DAOQJL010000485 DAOQJL010000000
VERSION     DAOQJL010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21850_length_3024_cov_3.759178"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLG78_16660"
     CDS             <1..706
                     /locus_tag="PLG78_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24806.1"
                     /translation="QDGSIRTSVYEGSVVTRARLNGVDDVPAELVENSEALKKIIKQI
                     EKSEKVLEPGDQATVAHFVVDAEVQNAIQTAITDPEVRALAEKKDRTPEDIKATAAAL
                     DKKLSPQLAVLEKKIQEDLPAAKVEVKERPPAPPGGYPVPDEFKEMQPVDASRSDTEE
                     KTRKEVEKRNQDENIRKNLMVRIEKVTGGKLDQLILKDGKTISGVIRQVGEDYIVTTP
                     EGNQSISGSQVGEMRF"
     gene            complement(740..>3024)
                     /locus_tag="PLG78_16665"
     CDS             complement(740..>3024)
                     /locus_tag="PLG78_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24807.1"
                     /translation="RFSSFLRLHPDVEPLIAGGFRLTNPLSAAYPSENLPFASRVIAS
                     GMWNYCFFQFYRHFAGPYWVERQYNPADPSFIPRASSMLSLNLTHRTWMGLRSPGGTR
                     FSMVDPAGSFSPMAGSYSIEFAVREGSKLLLATRGDLKVKQKPFKDMPVAKTEYSSRK
                     GFRATWIVAGCADQPGWVYSRIEYDAPGPCEIVIGIRPFNPEGAALITDLQLHMEPQG
                     AVLAINKNRDIFLRKSPDEVRFSNLKTGDAYFESEPHGIVHCPHGIATGCIIYRVSGH
                     GTIHFYARNDRTITEDIPLSFLANETEVHQSLASWERQIERGAKFISARGSWNKAARI
                     FSGYVLSLQTGREITPGVFTYNQFWFRDAAYMVSSLTAWGFIAEAKAVLESYAERQSR
                     DGFFKSHEGEWDSNGQAIWTLSHFIEHTGDLELLKKVYPAIRRGAQWIIKKRRQGPRR
                     LILPAGFSAEHLGPADHYYWDNIWSIAGLLHAAHLAGLMGETKDENYFTRECDEYRRD
                     LLEISSQERQKYGLITAAPDRPIDSGMIGNICAVYPLDLVLFPEDEMRRTVRQIHRDF
                     FTHDLFFHPIIHSGYNIYLSLQMAQSHLRLGDVRQARRIFKRVLKSKSELWTYPEAIH
                     PNTGGGVMGDGFHGWAFAEVLLLLREFVLHAQNGVLSIFRGLRNRELMGEFFFGPFPV
                     SAGRVSISGSMSNKRGRIQIDFDRASTMRSYRIHVPGIRSRIADIKGSGCASFHFDRK
                     KAIIEITGPTSEVEIELILS"
BASE COUNT          766 a          721 c          804 g          733 t
ORIGIN      
        1 tcaggatgga tccattcgca caagtgtcta cgaagggagt gtagtgactc gtgctcgcct
       61 caatggcgtg gatgatgttc ctgcggaact cgttgaaaat agcgaagcac tcaagaagat
      121 catcaagcaa atcgaaaaaa gtgaaaaagt tctcgaacca ggagaccagg cgactgtcgc
      181 acatttcgta gtggatgcgg aagttcaaaa cgcaatccag accgccataa cagatccgga
      241 agtgcgtgct cttgcagaga agaaagatcg cactccagaa gatatcaaag cgactgcggc
      301 cgccctcgat aagaagcttt ctcctcagct cgcagttttg gagaagaaga ttcaggaaga
      361 tcttcctgcg gcaaaggtag aagtgaagga aagaccaccg gctccgccgg gcggatatcc
      421 ggtgccagac gaattcaaag aaatgcagcc tgtagatgct tcgcgttcag atacagaaga
      481 gaagacgcgc aaagaagtag agaagcgcaa tcaggatgaa aacattcgca agaacctgat
      541 ggtgcgaatc gagaaagtta caggcgggaa gctcgatcag ctcatactca aagacggaaa
      601 aacaatcagc ggtgtgattc gtcaggtagg ggaagattac atcgtgacta cgccggaagg
      661 aaatcagtct atttcgggaa gtcaggttgg agagatgaga ttctgattta gattggaccg
      721 gcgggcaagt agcagccggt taagaaagga tcagctcgat ttctacttcg ctcgttgggc
      781 ctgtgatttc gatgattgct ttttttctgt caaagtgaaa ggaagcgcag ccgcttcctt
      841 tgatgtctgc gattctgctg cgaatccctg gaacgtgaat tctgtacgat ctcatggtac
      901 tggcccggtc aaaatcgatt tgaattctgc cgcgcttgtt gctcatgctt ccggaaatgc
      961 tgactcttcc agcggaaact ggaaatggac caaagaagaa ttcacccatc agttcgcgat
     1021 ttcgcaggcc gcggaaaata gaaagcacac cgttttgagc gtgcaaaaca aattcgcgca
     1081 gcaagagaag cacttctgcg aaagcccagc cgtgaaatcc atctcccata actccgccgc
     1141 cggtattcgg atgaattgcc tcggggtaag tccatagttc gctcttgctc ttgagtactc
     1201 tcttaaagat ccttcgcgcc tgcctcacat ctcccaggcg aaggtgcgat tgcgccattt
     1261 gaaggctgag gtagatgttg tagcccgaat gaatgattgg atgaaagaat agatcgtgcg
     1321 tgaagaaatc gcgatggatc tgtctgactg tgcgtcgcat ttcgtcttcc ggaaaaagaa
     1381 ccagatccag cggataaacg gcacagatat tcccaatcat tcctgaatca atgggacgat
     1441 caggcgcagc cgtgatcagg ccatacttct gtctttcctg ggaagagatt tctagcagat
     1501 cccgcctgta ttcatcgcac tcgcgcgtga agtagttttc gtcttttgtt tctcccatca
     1561 gtccggcaag atgcgcggcg tgaagaagcc ctgcaataga ccagatgtta tcccagtagt
     1621 aatgatccgc cgggccaaga tgctccgcgc tgaatccggc aggaaggatc aatcggcgcg
     1681 ggccctgtct gcgttttttg ataatccatt gcgctccccg acgaatggca gggtaaactt
     1741 tcttaagtaa ttcgaggtct ccggtgtgtt caatgaagtg cgacagagtc cagatggcct
     1801 gcccgttgga atcccattct ccttcgtgcg atttaaagaa accatcgcgt gactggcgct
     1861 ctgcatagct ttcgagtact gcttttgctt ctgcaatgaa tccccaggca gtcagggaag
     1921 acaccatgta tgctgcgtcg cgaaaccaga attgattgta agtgaataca cctggagtga
     1981 tttcgcgtcc ggtttgtaag gagagaacgt agccggaaaa tattcttgct gctttgttcc
     2041 agcttcctct tgcagaaatg aatttggccc cgcgttcgat ttgtctttcc cagcttgcca
     2101 gggattgatg aacttctgtt tcgtttgcga gaaaggagag aggaatatct tccgtgatgg
     2161 ttctatcatt tcttgcgtag aaatgaatgg ttccgtgacc gcttactcga tagatgatgc
     2221 agccagtggc aattccgtgc gggcagtgca cgattccgtg cggttcggat tcaaagtagg
     2281 cgtctccggt tttcaaattc gaaaaccgaa cttcatccgg gctctttctc aaaaagatat
     2341 cgcggttctt gttgatggca agtaccgccc cttgcggttc catatggagc tgaagatctg
     2401 tgatgagtgc tgctccttcc ggattgaaag gacgaattcc aattacgatt tcgcatgggc
     2461 ccggagcatc atattctata cgagaatata cccagcctgg ttgatctgcg cagccggcca
     2521 caatccatgt cgcgcgaaac ccttttcgcg aggaatactc tgtcttcgca acaggcatgt
     2581 ccttgaaagg cttttgtttt actttgaggt ctcctctggt tgcaaggaga agttttgatc
     2641 cttcgcgcac tgcaaattcg atgctgtaac ttcctgccat tggactgaat gagccggccg
     2701 gatccaccat ggagaacctg gttccccctg gagagcgcag tcccatccac gtgcgatgcg
     2761 tcagattcag cgaaagcatg ctgcttgctc ttgggatgaa agagggatcg gccgggttgt
     2821 attgtctctc cacccagtat ggcccggcga aatgtctgta gaactgaaag aaacaatagt
     2881 tccacatgcc cgatgcgatg acgcgcgaag caaaggggag gttttcagaa ggataggctg
     2941 cggataacgg attcgttagg cgaaagccgc ctgcgatcag cggctccaca tccgggtgca
     3001 gtctcagaaa tgaactgaag cggc
//
LOCUS       DAOQJL010000486         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22158_length_2998_cov_4.387700, whole genome shotgun sequence.
ACCESSION   DAOQJL010000486 DAOQJL010000000
VERSION     DAOQJL010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22158_length_2998_cov_4.387700"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="PLG78_16670"
     CDS             complement(<1..1367)
                     /locus_tag="PLG78_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNE24808.1"
                     /translation="MLNHGIIGNERILALVSPDQSIDWLCMPRFDSPSVFARLLDGQN
                     GGCFQILESLGDADRTFAYVENTNILRSQILNEQQGYELLDFAPRIVEGLGVQSPVEI
                     VRILRPIRGRPRLRVVFDARPDYGKTKPAVALSGESIVIRGRDMDFFLHSNLPLSFVL
                     EGAEFFLDRPVFFVFSCYSRAKRPDLAEVNFLLESTIQGWRSWAKTCSLPTFEPALVL
                     RSALCLKLHTYKDTGAVIAAATTSIPEAIGTQRTWDYRYCWLRDAAFVVEALRRLSHL
                     AEGEAFIRYLCNVVESGPLQPVYALDGARKIDEEFLPHLVGYKGNGFVRIGNAAHLQK
                     QNDLMGEIILCLETFVTDPRIVIDDGSAIFALVERLVESAIANAPEEDTGIWEFRSML
                     RPYTFSRAMCWAAIHRGAVIARRLNHPAHASRWQQIADIERATVLDRGYNKDLGFFTQ
                     ALGGE"
     gene            complement(1513..2793)
                     /locus_tag="PLG78_16675"
     CDS             complement(1513..2793)
                     /locus_tag="PLG78_16675"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNE24809.1"
                     /translation="MMNTPADPANERLRFRRPIFVVVTLLLVFGAIRLMDTVGAFFLD
                     TSAQYYVLPPGNVALGTNSLGLRNRELGPKAGTRILFLGDSFTQGLGVLEEDGFVRRS
                     EKALRQRFQVEHVNAGMTGFSPGNELGLLRAIREKVQPDIVVLCLYQNDVYESGESLL
                     LRRMKQNQQKKLGVRILFWIAPNTANFVYRTFLQKTYNDLLMSQALTLDRTQAMLIAS
                     DSSREKQSQVVPASAFQPALALIQSEVRKYAKEMGADESVTDRWLAQNGQALVDGRNP
                     QEVLLGLLEPEYFSQVYGLDARARPKFETMLKTIQRIKEETEQSGAAFAVVYIPSDVR
                     YDKSKFDLMRSYGYAMDESWLHDRVPMGIELRHRMEKENIPFLSLLDTFRRAPGPLTF
                     PRDQHLNRHGHDVAAVAVAHFLEASFQSRIKPKN"
     gene            complement(2815..2967)
                     /locus_tag="PLG78_16680"
     CDS             complement(2815..2967)
                     /locus_tag="PLG78_16680"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNE24810.1"
                     /translation="MDFLAEFFIFLRERRKFWIVPILLFLVVLVGLGIVATSAGGLSP
                     FIYALF"
BASE COUNT          758 a          849 c          724 g          667 t
ORIGIN      
        1 tattcgccgc ctaacgcctg cgtaaaaaac ccgagatcct tgttgtatcc cctatcgagc
       61 accgtggcac gctctatatc cgcgatttgt tgccaccgcg atgcgtgcgc cggatggttc
      121 aaccgccgcg caatcactgc gccccgatga atggctgccc agcacatcgc tcgcgaaaat
      181 gtgtaaggcc gcagcatgct gcgaaattcc cagatgcccg tgtcctcctc gggcgcattt
      241 gcaatcgctg attcgacgag acgctcgacc agcgcaaaga tcgcactacc atcatcaatt
      301 actatgcgcg gatcagtgac aaaagtctca agacacagga tgatctcgcc catcagatca
      361 ttttgtttct gaagatgtgc cgcattgcca atccggacga aaccatttcc tttgtaaccg
      421 actagatgag gcagaaattc ttcgtcaatt ttacgggctc cgtcgagcgc ataaaccggt
      481 tggaggggcc cggactcgac aacgttgcag agataacgaa tgaacgcttc tccctccgct
      541 agatgactca acctgcgaag agcttccaca acgaatgcag catctcgcag ccagcaatat
      601 cgatagtccc aggtgcgctg tgtaccgatg gcttccggta tacttgtcgt cgccgcggca
      661 atcactgcgc ctgtgtcttt gtatgtatgg agctttaggc acaacgcgga acgcaagacg
      721 agcgccggct caaacgtagg cagagaacat gtcttcgccc agcttcgcca gccttgaatc
      781 gttgactcga gcaaaaaatt cacttcagcc aggtccggtc tttttgcgcg agaatagcag
      841 ctgaacacaa agaacactgg ccggtccaaa aagaactcag cgccctcaag aacaaaagaa
      901 agggggagat tggaatgcag aaaaaaatcc atgtctcttc cgcggattac aatcgattca
      961 ccgctgagcg cgactgctgg ttttgttttt ccatagtcgg gacgcgcatc aaagacaact
     1021 ctcagtcgag ggcgtcctcg gatcggtctc aggatgcgca cgatttcaac gggcgactgc
     1081 acacccagac cttccacaat acgcggagca aagtccagaa gctcatatcc ttgctgctca
     1141 ttcaaaattt gagatcgtag aatatttgta ttctctacat atgcaaaagt cctgtctgca
     1201 tcaccgagcg attccagaat ctgaaagcat cctccgttct gcccatccag caaacgtgca
     1261 aaaacggaag gggaatcaaa tcggggcatg caaagccagt cgatcgattg atcgggagat
     1321 acaagcgcca gtattcgctc atttccgata atcccatgat tcagcaaacc gtggtcctct
     1381 tttgagcaat cccggaggac atgatggatg aagtgcgacg atgatgacga tgaggatgca
     1441 tttcctgttc cagtagcggc gcgacgtcct tctcactttg ccccagtggc ttgtgcgaaa
     1501 atcccgtccg cgctaattct tcggcttgat tcgagattgg aagctcgctt cgagaaaatg
     1561 tgccaccgct acggcggcca cgtcgtgtcc atgtcggttc agatgctggt ccctcggaaa
     1621 agttagaggg ccaggagcgc ggcgaaacgt atcaagcaag ctcaaaaacg gaatgttctc
     1681 tttttccatt cggtggcgta gttcaatacc cattgggaca cgatcgtgga gccacgactc
     1741 gtccattgca tatccatagc tccgcatcag atcgaacttg ctcttatcgt acctcacatc
     1801 ggaaggtata taaaccacag cgaacgcggc accgctttgc tccgtctctt cttttatcct
     1861 ctggatggtt ttcagcatgg tctcaaattt cgggcgcgcc cgcgcatcga gaccatatac
     1921 ttgcgagaag tattccggtt ccagcagacc gagtaagacc tcctgtggat tccgaccatc
     1981 cacaagcgct tgcccatttt gtgcgagcca ccgatctgta acactttcgt cggcacccat
     2041 ctccttcgca tactttcgca cttcgctctg gatcagcgcc agcgccggtt gaaacgcgga
     2101 cgccggcacc acttgcgact gcttttccct gctcgaatcg ctggcgatga gcatcgcctg
     2161 tgtccgatcg agcgtcaaag cctgactcat cagcaaatcg ttataagttt tctgaagaaa
     2221 cgtacgatag acgaagttcg ccgtgttcgg cgcgatccag aaaagaatcc gcacgcccag
     2281 cttcttttgc tgattctgct tcatccgtcg cagcagcaaa ctttcgcctg actcataaac
     2341 atcgttctgg tagaggcaga ggacaacaat gtcgggctgc accttctctc taatcgcgcg
     2401 caagagcccc agctcatttc ctggcgaaaa gccagtcatt ccagcgttca catgctccac
     2461 ctggaaacgt tggcgcagcg ctttctcaga gcgccggaca aaaccatcct cttcgagaac
     2521 tccaaggcct tgagtgaaag aatccccaag gaaaagaatc cgcgtccctg ctttgggacc
     2581 gagttccctg ttgcgcaagc ccagagaatt cgtgccgagt gctacatttc ctggaggaag
     2641 aacgtagtac tgggcagacg tatccagaaa gaaagcgccg accgtgtcca tcaggcggat
     2701 cgcgccaaag accagcagca atgtcacaac aacgaaaata ggccgccgaa atcggaggcg
     2761 ctcgttcgca ggatcagcag gcgtgttcat cattgtgttt ctcctcgacg aatgctagaa
     2821 tagggcatat atgaaagggg aaagtccgcc tgcactcgtt gcgacaatgc caagtccaac
     2881 cagaactacc aggaacaaca aaatgggcac aatccagaac ttacggcgct ccctgagaaa
     2941 aatgaagaac tctgccagaa aatccatata ctccccttaa aaatgatgat cgtaagtt
//
LOCUS       DAOQJL010000487         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22184_length_2996_cov_3.507650, whole genome shotgun sequence.
ACCESSION   DAOQJL010000487 DAOQJL010000000
VERSION     DAOQJL010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22184_length_2996_cov_3.507650"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="PLG78_16685"
     CDS             complement(<1..1729)
                     /locus_tag="PLG78_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE24811.1"
                     /translation="MRISFGLKLAFATSMLAFVTTSAGLGYFYKITRQNVWNELAERL
                     ADMGRTGQFIFSAEHRRAIDRLNELSEKDSSRNKDHLSIGPGESISVISGSRVHEIEK
                     SDDFILVVQALRQIKNASRKGMVNLGILGQEPWPKDDPPRVRFTYLLVPIPEVKDRSI
                     LKFIADADYEAGDENGNGKIDPEEEASEIGKLYNVSAQPGLMAAFDGKVRTSEEYYED
                     QWGKWMSAYVPILRADGSVAAVMGIDMSATSQFNLLEKLYYICIAVIGGSVVLAIILS
                     LALSRLIASPIVALRAGAERVKARDYSTQIVVKSKDELGILASTFNDMVREVRTYATT
                     LEDRVKERTAELNESRGQMSDILRNIQEGILTIDRSGKVNSEYSGRVPDMLGKQPEGL
                     EFADLFPAQLQNSVRDFVGQLIENPFMSMRMFAAANPLSEVRILSGDQEKVLSFSFSR
                     VKNGAGQIEKLLVVIDDRTEQMKLERALAEREAEQSARIEKLYQILNLDPNVFKSFLE
                     EAGRTADVVVEKLALAGSDRSALDLCFREVHTLKGNARALGLDAIAKNAHELEDVLDA
                     IRKDPESSAQ"
     gene            1840..>2996
                     /locus_tag="PLG78_16690"
     CDS             1840..>2996
                     /locus_tag="PLG78_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNE24812.1"
                     /translation="MDYSQKSLIIHEQLRGKIGVSSKMKIETMDDLSVAYTPGVAAPC
                     LAIAGNRELAKKITIKHNSVAVVTDGSAVLGLGNIGPEAAIPVMEGKALLFKQLADID
                     AWPVCLDTQDTDEIVETVRRIAPVFGGINLEDISAPRCFEVERRLQDLGIPVFHDDQH
                     GTAIVLLAGLLNACRITGKQISDLRIVINGAGAAGIAITNILRDDGPGDKIIAKDIIV
                     CDTQGAIHAGRTDLNSEKQELLRYTNFENRSGSIQEVMKGADVFIGVSKANLLTADHV
                     RTMAKDSMIFALANPVPEIMPAEAKLGGAAIIGTGRSDFPNQVNNALAFPGIFRGALD
                     AGARKITDEMKIAAARALAEMVKNPTPEHILPRVLDPAVSATVAKAVYKAA"
BASE COUNT          755 a          787 c          722 g          732 t
ORIGIN      
        1 cctgtgcact cgattctgga tctttgcgaa ttgcatcgag cacatcttcc agttcgtgag
       61 cattcttcgc aatcgcgtcg agaccgagcg cgcgagcgtt tcccttgaga gtgtgcactt
      121 ctctaaaaca caaatcaaga gcggatctgt ccgatcctgc aagagcaagc ttctcgacca
      181 ccacatccgc tgttcggcct gcttcctcca gaaaagattt aaaaacattg gggtccagat
      241 tcagaatctg atatagcttt tcaatgcgcg cagactgctc tgcttcgcgt tctgcaagag
      301 cgcgctccag tttcatttgt tccgtgcgat catctatgac aacgagcagc ttctctatct
      361 gtcctgcacc atttttcact ctcgaaaatg aaaaggaaag aaccttctcc tgatcccctg
      421 acaggatacg aacctcagaa agcggattgg ccgcggcgaa cattcgcatg gacatgaatg
      481 gattttcaat gagctgtcct acaaagtcac gaacagaatt ctgcaactga gccgggaaca
      541 aatctgcaaa ttccaaccct tccggttgct tgcccagcat atcgggaact cttcctgaat
      601 attcagagtt tacttttccg gatctgtcta tggtcaggat gccttcctga atgtttcgca
      661 ggatatcaga catctggccg cggctttcat tgagttccgc cgtacgttct ttaacacgat
      721 cttccagagt agtggcatac gttcgcactt cgcggaccat gtcgttgaac gtggatgcaa
      781 gaattccaag ctcatcctta ctcttaacta caatttgagt agaataatca cgcgctttca
      841 ccctttctgc gccggcgcga agagcaacaa tggggctcgc aatcaaacga gaaagagcaa
      901 gagaaagaat gattgcaaga accacgcttc caccaatgac cgcgatacat atatagtaaa
      961 gtttctcaag taagttgaac tgtgaggttg cactcatgtc aatccccatc acggccgcga
     1021 cgctgccatc cgctcgaaga atggggacat acgcggacat ccatttgccc cactgatctt
     1081 cgtaatattc ctcactcgtg cgcaccttgc catcgaatgc agccatgaga cctggctgag
     1141 cggatacgtt gtagagcttt ccaatctctg aggcctcttc ttctggatca atcttgccgt
     1201 tcccgttttc atctcctgct tcgtagtctg cgtctgcgat gaatttgaga atgcttctgt
     1261 ctttgacttc cggaatggga acgagcaagt acgtaaagcg cacgcgcggc ggatcgtctt
     1321 ttggccaggg ctcctgacct agaattccga gattcaccat ccctttgcgt gaagcattct
     1381 tgatctgtct gagagcctgg accaccagaa tgaaatcatc cgatttttca atttcgtgca
     1441 ctcgcgaacc ggagatcaca gagatgcttt cgccgggccc gatagacagg tggtctttgt
     1501 tgcgcgaaga atctttctct gacagttcat tgagccgatc gatcgcacgc ctgtgttctg
     1561 cgctgaatat gaactggccg gtgcggccca tatctgccag tctttctgca agttcattcc
     1621 agacgttctg tcgcgttatc ttgtaaaagt aacccaggcc ggcactggtg gtgacgaaag
     1681 ctagcatcga cgttgcgaaa gcaagcttca aaccgaatga aatgcgcata tcctaatctc
     1741 aaaacggaaa gctccctgtc gagtggttcg cgtcagaatg ccggcaaatc ggtcacaaaa
     1801 tttccattta tctccaggct cattttcagg aaatggcata tggattattc acaaaaatcc
     1861 ctgatcatcc acgagcagct tcgcggaaag atcggcgttt ccagcaagat gaaaattgaa
     1921 accatggacg atttgtccgt cgcctacact ccaggtgtgg cggctccctg tcttgcgatc
     1981 gcgggcaacc gggagcttgc caaaaaaatc accatcaagc acaactcagt tgctgtggtt
     2041 acagacggat ctgcagttct tggcctcgga aacattggac cggaggcagc cattcctgta
     2101 atggagggaa aagctctact cttcaagcaa ctggcagaca tcgacgcatg gcctgtgtgt
     2161 cttgatacgc aggacacaga cgaaattgta gaaacagttc gtcgcattgc acctgtattt
     2221 ggcggcatca acctcgaaga catatctgcg ccgcgttgtt ttgaagtgga gcgaaggctt
     2281 caggatctgg gcattccggt tttccatgac gatcaacacg gaactgccat tgtgctactg
     2341 gccggacttc tcaacgcctg cagaatcaca ggaaaacaaa tttcggatct tcgcatcgtg
     2401 atcaatggtg cgggagcggc aggcatcgcc atcacgaaca tactgcgcga tgatggtccc
     2461 ggagacaaga tcatcgcaaa agacatcatc gtgtgcgata ctcagggagc cattcatgcc
     2521 gggcgcacgg atctgaattc tgaaaagcag gaattgttac gttatacgaa ctttgaaaat
     2581 cgtagcggat cgattcaaga agtgatgaag ggtgcagacg tgttcattgg cgtgagcaaa
     2641 gcaaatctac tgactgcgga ccatgtacgc accatggcaa aagactccat gatctttgct
     2701 ctggccaacc ctgtccctga gatcatgcct gcagaggcta aactcggcgg agcagcgatc
     2761 attggtacgg gtagaagtga cttcccaaac caggtcaaca atgctcttgc ttttcctggc
     2821 atctttcgcg gagcgctgga tgcgggtgcg cgaaaaatca cagatgaaat gaaaatcgcc
     2881 gcagcacgcg ctctggcaga aatggtaaaa aacccgacgc ccgagcacat actgccccga
     2941 gttctggatc ctgcggtatc cgctacggtt gcaaaagcag tctacaaagc tgcgca
//
LOCUS       DAOQJL010000488         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22217_length_2993_cov_4.282505, whole genome shotgun sequence.
ACCESSION   DAOQJL010000488 DAOQJL010000000
VERSION     DAOQJL010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22217_length_2993_cov_4.282505"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..801)
                     /locus_tag="PLG78_16695"
     CDS             complement(76..801)
                     /locus_tag="PLG78_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE24813.1"
                     /translation="MQIKRQDRIVFLRSLLANFASVRVEGAANLPASGGAVIVCNHTD
                     LSDGLIQLLYTPRPLVFLAKSELFESGGNLRISDIIEGNEFFRQFPSDILADMMNLAG
                     QFIQDVDAMPVIRGYRGVHRRDAAAYYRELAESVVKRLTAGELVAIYPEGRRSTSGEL
                     LPFKGFAARMALLARVRVIPCCIQGNTGLASPRLMEKNSPRSVIYKIGQSILPEEFPE
                     GGGKYAVKRLTQKIEARVKELSG"
     gene            complement(832..2340)
                     /locus_tag="PLG78_16700"
     CDS             complement(832..2340)
                     /locus_tag="PLG78_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24814.1"
                     /translation="MPEETRQPDAPIDKKRIALMEGTCGVPDRVSIRGQVVDIPVTEK
                     EMKSDWDMFLGLPRIARETIRPLQDVGMGGVRRARLQAEVFPEHRDGVSPPLWVSDTF
                     STNDNSFFSTTAQKLSSGIYAVRLTLRGIDSLRQSVADFAFMPSRQSLTLKTDVIIGE
                     GRFRVLPRDYTGWIVTSDIDQTFLDTKLDSRGGLMQTLFEMPSEKRPLPGMAELMRKI
                     TDRNIPLHFISASPHFFRRSFNALFAFHNIPVTGLRLKYLQGVFEEARKKLFESLLHV
                     TDYLQAGVGGAMDRSMKYLNSSLQSLFDQVGYKLEAILENRLIQPTGSREILMGDNTE
                     SDYFIFTLYQVLLTGRLRDDQLVDYLYHLRFHGREALTRDYSVRIAKLTTENLQIHGK
                     INPVEHVWINQAYASPDNEEAEKMIREALPEDAFKWGTIKKPIVVKGGLGFAVHALDV
                     GLIDPDQFRAVWKSMEGRKLGELELNQAFLETIVKSMPWTRYSAEQVLGYAV"
     gene            2406..>2993
                     /locus_tag="PLG78_16705"
     CDS             2406..>2993
                     /locus_tag="PLG78_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE24815.1"
                     /translation="MQKKNSEPLPIFVYSSDYDMDLGPHVFPSVKFGFLYSRLVDNSK
                     FKKHKFVEPEPLTPQEAALVHSRAFLADLNSLTMSNRLHRSELPLNREIVDAFYLASG
                     GTALAARLALESGLAMNLSGGFHHSYADHAEGFCYLNDVAISVRMLQKEKKIKRALII
                     DLDVHQGNGNAKIFHGDNTVFTFSMHEQNNYPVKEQ"
BASE COUNT          769 a          799 c          712 g          713 t
ORIGIN      
        1 taaaatatcc tatcatcagc attccgccga agaaaagaaa tccagtcaga agagttaaga
       61 atcgatgaga aaacatcagc ccgagagctc cttcactctg gcttcaattt tttgcgtcaa
      121 tcgtttcact gcatacttgc caccaccctc cggaaactct tcgggcaaga tcgattgtcc
      181 gattttgtaa atcacggatc gcggagaatt cttttccata agacgaggac ttgccagacc
      241 cgtgttgccc tgaatgcagc agggaattac acgcactcga gcaagcaatg ccatgcgagc
      301 cgcaaatcct ttgaacggaa gaagctctcc agatgtagat ctcctgcctt ccggatagat
      361 ggcaacgagc tcccctgccg tcaggcgctt caccacactt tctgcgagtt ctctataata
      421 agcagcggca tcacgcctgt gcactccgcg ataccctcga atgactggca tggcatctac
      481 gtcctggata aactggcccg cgaggttcat catatccgcc agtatgtcag atggaaactg
      541 acgaaagaat tcgttgccct ctatgatatc agagatccgc agatttcccc cggattcaaa
      601 gagttccgat ttcgcaagaa acacgagagg gcgcggagta tacaggagct ggatgagtcc
      661 gtctgaaaga tccgtatgat tgcagacaat gaccgcgcca cctgacgcgg gaagattggc
      721 cgcaccttcc actcgaacgc ttgcgaaatt tgcgagcaag ctgcgaagaa aaacaatcct
      781 atcctgtctt ttgatctgca ctactgaatt gccgcgggca aaagtaagac gttagacggc
      841 atatccgagc acctgctccg cactatacct ggtccagggc atggacttta cgatggtttc
      901 caggaatgct tgattcaatt ccagttcacc gagtttgcga ccttccatgg atttccacac
      961 tgcgcgaaat tgatccggat cgatgagacc aacgtccagt gcgtgcactg cgaatccaag
     1021 tccacccttg acgacgattg gctttttgat ggttccccat ttgaatgcgt cttctggaag
     1081 ggcttcgcga atcattttct ctgcttcctc attatccgga gaagcgtacg cctgattgat
     1141 ccatacatgt tctacaggat tgatcttacc atgaatctgg agattctccg tcgtgagttt
     1201 tgcaatgcga acagaatagt cgcgagtgag cgcttcgcgg ccgtgaaatc gaagatgata
     1261 gagatagtcc acaagctggt catcgcggag tctgcccgta agtagaacct ggtagagagt
     1321 gaaaatgaaa tagtcacttt ccgtgttatc tcccatgaga atttcgcgcg aacccgtagg
     1381 ctggatcaaa cggttttcaa gaatcgcttc taatttgtat cccacctgat cgaagagact
     1441 ctgcagactt gagttgaggt acttcatgga tcgatccatg gcaccgccga ctccggcctg
     1501 aagataatcc gtaacgtgaa gtagactctc aaacaatttc tttcgcgctt cttcgaatac
     1561 tccctgcaaa tacttcaggc gaagacctgt gactggaata ttgtgaaacg caaacaatgc
     1621 gttgaagctc ctgcgaaaga aatgcgggga ggcagaaata aaatgcagag gaatattacg
     1681 atccgtaatc ttcctcatga gttcggccat accgggaaga ggtcttttct cggaaggcat
     1741 ttcaaagagg gtctgcatga gaccgccgcg agaatcgagc tttgtatcca ggaacgtctg
     1801 atcaatgtct gaagttacaa tccatcccgt atagtcccgg ggaagaactc gaaatctacc
     1861 ttctccaatg atcacatccg tcttcagagt cagactctgg cgtgaaggca tgaatgcaaa
     1921 atcagcaaca ctctggcgaa gagaatcaat gccgcgcaag gtcagacgaa ccgcatagat
     1981 tccagaagag agcttttgtg cggtcgtgga aaagaaacta ttgtcattgg tagaaaatgt
     2041 atctgaaacc cagagaggtg ggctgactcc gtcccgatgt tctggaaaaa cctccgcctg
     2101 aaggcgtgcc cggcgcactc cacccatacc tacatcttgt agaggtcgaa tggtttcccg
     2161 ggcaatcctg ggcagcccga ggaacatgtc ccagtctgac ttcatttcct tttctgttac
     2221 gggaatatcc actacctggc cgcggataga aacgcgatcg ggcacaccgc acgtaccctc
     2281 catgagggcg atgcgtttct tgtctatggg tgcgtctggt tgtcgggtct cttctggcat
     2341 gcttcgcttt actgttttac agattcaagg gccgcttcac attgaccgaa cgtacctctg
     2401 gcgtcatgca aaaaaagaac tcagagcctt tgcccatctt cgtttactcg tcggactacg
     2461 acatggacct ggggcctcac gttttcccgt ccgtgaagtt cggctttctt tattcacgcc
     2521 tcgtggataa ctccaaattc aagaagcaca aattcgtcga accggagccc ctgacgcctc
     2581 aagaagccgc cctggttcat tccagagcat tcctggcaga cctcaactcc ctgaccatgt
     2641 ccaatcgcct tcacagaagt gagctcccgc tgaaccgcga gattgtggac gcattctatc
     2701 tcgcaagcgg cggaacggct cttgccgcaa gactcgccct tgaatccggt ctggccatga
     2761 acctcagtgg tggctttcat cactcgtacg cagatcacgc ggaaggtttc tgctacttga
     2821 acgacgtggc catcagcgtt cgcatgcttc agaaagaaaa gaagatcaaa cgcgcgctca
     2881 tcatcgacct ggacgttcac cagggcaacg gcaatgcgaa gatctttcac ggagataaca
     2941 cagtctttac cttctccatg cacgaacaaa acaactaccc cgtcaaagaa cag
//
LOCUS       DAOQJL010000489         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22335_length_2984_cov_3.570843, whole genome shotgun sequence.
ACCESSION   DAOQJL010000489 DAOQJL010000000
VERSION     DAOQJL010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22335_length_2984_cov_3.570843"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="dapF"
                     /locus_tag="PLG78_16710"
     CDS             complement(<1..821)
                     /gene="dapF"
                     /locus_tag="PLG78_16710"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNE24816.1"
                     /translation="MVHLPFFKMEGAGNDYVYLDALEDPALVLKLTPEMIARMSDRHF
                     GIGGDGVVMILPSGDSEALMRMWNADGSESSMCGNALRCVAFYVHKKNGKRNFTIETK
                     SGRHAALIVESSAKHGTVQVQIGQPRTGPDEIPVNPDVLGLTGKGPFLDVPVSVSGFE
                     GKGTLVSMGNPHCVFFVEDPDSLDLEKIGPLLEHHPAFPARTNVEFVCVQSDGTIRQR
                     TWERGSGETLACGSGVCAVLVASVLSRNMPRKNTIHVRGGDLEASWTEDGVMLSGP"
     gene            935..1693
                     /locus_tag="PLG78_16715"
     CDS             935..1693
                     /locus_tag="PLG78_16715"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNE24817.1"
                     /translation="MLENDPLQNLPGDYYRTEDLREFMNASASWMMRYIQIRLTRDPD
                     RAGDFYLHFYERAARCLEAYRSRQHLPFTGFLATYLRHEFMNFLRTAKTREGSEVKAD
                     DMSYFPAADERPPSPESDVPADLPLNCRIPLKLHSGLHLNSEEIRSLAVELGPGATAS
                     LLKEAMQRRMSRDRRTQKLEDRAARLNYLIHTSKAPASRLKKWKNRIVAMLDRPRPIF
                     SYSELAERLGTSKTTISRRIDQARTGVRVIQEAL"
     gene            1690..2256
                     /locus_tag="PLG78_16720"
     CDS             1690..2256
                     /locus_tag="PLG78_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24818.1"
                     /translation="MNLQKNASSLSNPMEILEALLPAGRMPASMLRSDQNTQEMQEIL
                     ADMAACDLARVPARPHFFKANKNGLVSSFGLRPSLAFRSKSRIVHSDIIALDKDYLIV
                     ELSADETGLHIVMEKGAKNESTISISLYRGAAAVEFLHIKSRVRKDLGELAPGEYSLI
                     CENRPVLNFSLFDENENHNKRRSNNRKD"
     gene            2207..2548
                     /locus_tag="PLG78_16725"
     CDS             2207..2548
                     /locus_tag="PLG78_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE24819.1"
                     /translation="MKTKITTKGEVTIVKIEGAIKSGDEYELAEKMEKYIKPDSAPKF
                     IVDMKKVPFINSAALGLFLNIFKHVDYLKGRMVFAGLNSDVENLMEITKLSSVFEIFK
                     TVEEALESFDF"
     gene            complement(2568..>2984)
                     /locus_tag="PLG78_16730"
     CDS             complement(2568..>2984)
                     /locus_tag="PLG78_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24820.1"
                     /translation="NSQMLIFFIPMRARNAAILIGVLSIVLPMFGVLTFISHMGHLGG
                     LVGGVLYSWFALGLRFGQNDLRERESYIRRKVQEQEYLREMLQRLQKASPRFQGTSRE
                     RWKEPDEKVINPRPEDVQGPEKRLYYDPTTGKFYTR"
BASE COUNT          780 a          778 c          726 g          700 t
ORIGIN      
        1 ggaccggaga gcatcacacc atcttccgtc cagcttgctt ctagatcccc gcctcgaaca
       61 tgaattgtat ttttccgagg catgttgcga gatagaacgc tggcgacgag cacagcgcag
      121 acgccactgc cacacgccag ggtttccccg cttcctcttt cccacgttct ctggcgaatc
      181 gtgccgtctg attgcacgca gacaaattca acgttggttc gcgccggaaa agcaggatgg
      241 tgttctagaa gtggtccaat cttttcgagg tcgagactgt ccggatcctc cacaaagaaa
      301 acacaatgag gattgcccat agaaactaaa gttcccttcc cttcaaatcc agacacagaa
      361 acaggcacgt ccaggaaagg tcctttcccg gtaagtccga gcacatccgg atttaccggg
      421 atttcgtctg gcccggtgcg aggctgaccg atctgcacct ggactgttcc atgctttgcg
      481 gagctctcga cgatcaatgc cgcatgacgt ccacttttcg tttctattgt aaagttccgc
      541 ttcccatttt ttttgtgaac atagaaagca acgcatctga gagcatttcc acacatggaa
      601 gattctgatc catctgcatt ccacatgcgc atgagtgctt ccgagtcacc ggaaggaaga
      661 atcatcacta caccgtctcc gccgattcca aaatgtcgat cgctcatgcg cgcaatcatc
      721 tccggagtga gtttcaaaac cagagccggg tcttcgaggg cgtccaggta gacataatca
      781 ttcccggcgc cctccatttt aaagaaagga aggtgcacca ttacacaaaa agcgccaggg
      841 cctgggcggg cacttttttt tcccggattt gttttttgat cgaaaaagcg cggaacgatt
      901 ttccattgct ccacccttct ttttttgaac gaccatgcta gaaaatgatc cgctacagaa
      961 cctgcccggg gactactatc gcacagaaga tctcagggaa ttcatgaacg ccagcgcttc
     1021 ctggatgatg cgctacatcc agattcgcct cactcgcgat ccggaccgtg caggagattt
     1081 ctatcttcat ttttacgaaa gggcggcccg ctgcctcgaa gcttacaggt ccaggcagca
     1141 tctgccgttt accgggtttc ttgctaccta cctgcgccac gaattcatga attttctgag
     1201 aaccgccaaa acaagagaag ggagcgaagt caaagcggat gacatgtctt actttcctgc
     1261 ggcggatgaa agaccgccgt cgcctgaatc ggatgttccc gcggaccttc cgctgaactg
     1321 tagaatcccg ctgaaacttc attccggact tcatctgaac tcagaagaaa tcagaagcct
     1381 ggcggtagaa ctgggtccgg gtgcaacagc gtcccttctc aaagaggcca tgcaaagaag
     1441 aatgtctcgc gatcgccgca ctcagaagct ggaggaccga gcagcccggc taaattactt
     1501 gatacatacg tcgaaagcac cggcgtcccg cttgaagaag tggaagaatc gaatcgtggc
     1561 aatgctcgat cgaccgcgtc ctattttttc ttactcagag ctcgcagaaa ggctcggtac
     1621 cagtaaaact actatatcca gaagaatcga ccaggcccga acaggagtcc gggtgatcca
     1681 ggaggcacta tgaatcttca aaagaatgca agctctctgt caaaccccat ggagatcctc
     1741 gaggccctgc ttccggcggg cagaatgccc gcgagcatgc ttcgctcgga tcagaacacg
     1801 caagaaatgc aggaaatcct cgcagatatg gcagcctgtg atcttgccag agtgccagcc
     1861 cggccccact ttttcaaagc gaacaagaat ggtctggtat cctccttcgg gcttcgcccg
     1921 tccctggcct tccgttcgaa gtcacgcatc gttcacagcg acatcatagc cctggacaag
     1981 gactatttga tcgttgaact gagcgcagat gagactggtt tgcacatcgt gatggagaaa
     2041 ggcgcaaaaa acgagagcac tatatcaatt tcactttaca gaggcgccgc agcggtagaa
     2101 ttcttgcaca taaaaagcag ggttcggaaa gacctgggtg agcttgcacc gggagaatac
     2161 tccctgatct gcgagaatag gccagtactg aattttagtt tatttgatga aaacgaaaat
     2221 cacaacaaaa ggcgaagtaa caatcgtaaa gattgagggc gcgattaaat cgggcgatga
     2281 gtacgagctg gcggaaaaaa tggaaaagta catcaagcca gacagcgctc caaaattcat
     2341 tgtggacatg aaaaaagtgc ccttcattaa ttctgcggcg ctcggcttgt tcctgaatat
     2401 attcaagcac gtagattatc tgaaaggtag aatggtgttt gcgggcttga attcagatgt
     2461 cgaaaacctg atggagatta cgaaactcag ttcagtgttt gagatcttca agaccgtaga
     2521 ggaagcgctc gaaagtttcg atttctaagt ttgcttcttt caaaattcta tctggtgtag
     2581 aactttccag tagtggggtc atagtagagt ctcttctctg gcccctgcac gtcttcggga
     2641 cggggattga tgaccttctc gtctggttcc ttccagcgtt ctctggaagt tccctggaat
     2701 cgaggggatg ctttttgcag tctctggagc atctcgcgca aatattcctg ttcctgcacc
     2761 tttcgcctga tgtaactttc tctttcgcgc agatcattct gtccaaatcg aagtcccaga
     2821 gcaaaccagg aatagagcac tcccccgacc agtccgccaa gatgacccat gtgagaaatg
     2881 aaagtcaaca caccaaacat ggggagcaca atcgaaagaa cgccaatcaa gatggcagcg
     2941 tttctcgcac gcatgggaat aaagaagatg agcatttgtg aatt
//
LOCUS       DAOQJL010000490         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22365_length_2981_cov_3.955571, whole genome shotgun sequence.
ACCESSION   DAOQJL010000490 DAOQJL010000000
VERSION     DAOQJL010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22365_length_2981_cov_3.955571"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLG78_16735"
     CDS             <1..656
                     /locus_tag="PLG78_16735"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNE24821.1"
                     /translation="NSAIFGFIKKETRARTFAPRDLRFRISDKVYGPSHHGTVPIAIG
                     SLTLDTPADGFVQANSMLIETWLKFIASLIGPGLPPALELYCGSGIIGAYVRENLGSY
                     RAFDFNSSSIECARKNFSNSGLEGQFDVMDLNEEMPEPASLCIVNPARPGLSACVREG
                     LKASSVRSIIYSSCNPATLNRDLVELLAAGFFVRKSAMFDFFPRTPHVEMVCLLERK"
     gene            653..1147
                     /locus_tag="PLG78_16740"
     CDS             653..1147
                     /locus_tag="PLG78_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24822.1"
                     /translation="MRWFVVLCLFAPFCTPSALIEKKYATYNFTEEGFLSPRLIQTIG
                     KSRYPAGSYTIDSSRMRCLENSLEMARERMLRIFVFTRFGLKARKTKDQRTFDDDYPH
                     ALSARTLRRAEADFSVLLDRATIALQDARSQESCIVVYRLAGDSLPEEIKAVQTTFVP
                     DLAP"
     gene            1171..1527
                     /locus_tag="PLG78_16745"
     CDS             1171..1527
                     /locus_tag="PLG78_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24823.1"
                     /translation="MFVLACSSREVQVPEDTDLTEGMISSSTYQVVLSAFGADEQEAR
                     KRGEEDARRKTYMLILREPFFRGTLSEEGKRQIRTLIETRGRVVSVQKEAEGAYRLIF
                     QVTQPGGLRNTFRSIR"
     gene            1567..>2981
                     /gene="gltX"
                     /locus_tag="PLG78_16750"
     CDS             1567..>2981
                     /gene="gltX"
                     /locus_tag="PLG78_16750"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNE24824.1"
                     /translation="MSSPIRTRFAPSPSGFLHVGGARTALFNYLYAKARGGQYVLRVE
                     DTDQARSTRESERMILESLNWLGIVPDEGPDEGGPYGPYRQSERLDIYKKYTTKLLEE
                     GLAYPCFCTEEELEQMQESSRKLGIPNIYDGRYANLPKEEAEARIKAGDPYSIRLRVG
                     AEEVVVNDLVQGKVKFDSRLIGDFIIVKSDGFPTYNYAVVIDDNEMKISHVIRGVGHL
                     SNTPRQILIHRALGLDLPQYAHISEIVGTDKKKLSKRRGATSILFFRDLGYLPEAFNN
                     YMALLGWYPEDGAEFMSSADLKRKFDVARCSKSPSMFDFFLTDKGGDAEDAKPEGMTL
                     DELKKAINKKTKLNWLNNKYIRAADLDEIWPEAARFLSRDKELADAVKKDPDKMRKIF
                     SEVRIYLDTFDDAAPFYRELLKQKIALEPGEAAEVLALPEAKPVIEAFKQIVKETRPT
                     TPEGFSEAMKQAGVQTGAKGRA"
BASE COUNT          779 a          689 c          786 g          727 t
ORIGIN      
        1 tgaatagtgc gatttttgga ttcatcaaga aagaaacgcg cgcgcgaaca tttgctccca
       61 gagatcttcg ctttagaata tcagacaagg tctatggccc atcgcaccac ggtacagtgc
      121 cgattgcgat tggatcactg actctcgata ctccggcaga tggatttgtt caggccaatt
      181 ccatgctcat tgaaacctgg ctgaaattca ttgccagtct gattggtcct ggtctcccgc
      241 ctgctctcga gctctattgt ggcagcggaa tcattggggc ctacgttcga gagaacctcg
      301 gaagttacag agcgtttgat tttaattcct cgagcattga atgtgcgcga aagaattttt
      361 ctaactcagg actggaaggt caatttgatg tgatggatct gaatgaagaa atgccggagc
      421 ctgcatctct ctgcattgta aatccagcga ggccaggtct cagcgcctgt gttcgcgaag
      481 gcctcaaagc cagcagtgtg cgaagtatca tctattcttc gtgcaatcca gccactctaa
      541 acagggatct agtggaacta cttgcggctg gtttttttgt ccgaaaaagc gcaatgtttg
      601 acttttttcc aagaaccccc cacgtggaaa tggtttgtct cctggaaaga aaatgaggtg
      661 gttcgtcgtt ctgtgtttgt ttgcgccctt ctgcacgcct tctgcactca ttgaaaagaa
      721 atacgcgacg tataatttta cagaagaagg ctttctttcg cctcgtctca tccagaccat
      781 tgggaagtcc agatacccgg ccggatccta tacaatcgat tcgtctcgca tgcgctgcct
      841 cgaaaattcc ctggaaatgg cgcgggagag aatgcttcgg atttttgttt ttactcgttt
      901 tggactgaag gcccgcaaga caaaggacca gcgcaccttt gacgatgact atccgcacgc
      961 actttctgcc cgcacattga ggcgagcgga ggcggatttc tctgttttat tggacagggc
     1021 aaccattgct ttacaggacg ctcgatcgca ggaaagttgt attgtcgtct atcgtctggc
     1081 cggagattcg ttgcctgaag aaatcaaggc agtgcaaacg actttcgtgc ccgatctggc
     1141 accatgagaa aactcgctat ttgcttatca ttgttcgtac ttgcatgttc ctcccgcgag
     1201 gtgcaggtac cggaagatac agacctgacg gaggggatga tctccagctc cacctaccag
     1261 gtggttctat ctgcatttgg tgcggacgaa caggaagccc gcaagcgcgg ggaagaagat
     1321 gcgcggagaa agacatacat gttgattctg cgagagccat tctttcgcgg cactctgagt
     1381 gaagaaggaa agaggcaaat tcgcacactc attgaaacac gcggccgcgt tgtgagtgtg
     1441 cagaaggaag cggaaggcgc ctatcgtttg atctttcagg taacgcagcc aggcggcctt
     1501 cgcaacacgt tccgttccat tcgatagaac atagttttga ttcaatgatg atttgagagg
     1561 aatacaatga gttcacccat tcgcacgcga tttgccccgt ctccttctgg atttctccat
     1621 gtcgggggag cgcgcactgc tttattcaac tatctatacg ccaaggcacg cggaggccag
     1681 tacgtattgc gtgtggaaga tacggatcag gcaagatcga cgcgcgaatc agagagaatg
     1741 attcttgaat ctcttaactg gctggggatt gttccagacg aaggtccgga tgaagggggt
     1801 ccctatggac cttacaggca atctgagcgc ctggacatat acaagaagta cacaacaaaa
     1861 ctgctcgaag aagggctcgc ttatccttgc ttttgcacgg aagaagaact cgagcaaatg
     1921 caggaaagct cgcgcaagct tggaatacca aacatatatg atggacgtta tgcgaacttg
     1981 ccaaaggaag aggcagaggc tcgcatcaag gccggtgatc cgtattcgat ccgtcttcgc
     2041 gtcggtgcgg aagaggtagt ggtcaatgat ctggttcaag gaaaagtaaa gttcgattcc
     2101 aggttgatag gagacttcat cattgtgaag tcagacggat ttccaaccta caattacgcc
     2161 gttgtcattg acgacaatga aatgaagatt tcgcatgtga ttcgaggtgt cggacatctt
     2221 tccaacacgc cgcgacagat tttgattcat cgcgcactgg ggcttgatct tccgcaatac
     2281 gcgcatattt ctgaaattgt tgggacagac aagaagaagc tttcaaaaag aagaggggct
     2341 acctcgattc ttttcttcag agacctgggg tatttgccgg aagcattcaa caactacatg
     2401 gcactcctgg gatggtatcc cgaggacggt gcggaattca tgtcttcggc ggatttgaag
     2461 cgcaagttcg atgtggcgcg ttgctccaag agtccttcca tgttcgattt ctttttaacg
     2521 gacaaaggag gggacgcaga ggatgcgaag ccggaaggaa tgactctgga cgagcttaaa
     2581 aaggcaatca acaagaagac aaagctaaac tggctcaaca acaaatacat tcgtgcagcg
     2641 gacctggacg agatctggcc tgaggcagcg cgctttcttt cgagagacaa agagctagca
     2701 gatgcagtga agaaagatcc ggataaaatg cgtaagatct tttcagaagt gagaatttat
     2761 ctggacacct tcgatgatgc agcgccattc tatcgtgagc tgctcaagca aaagattgca
     2821 ctggagccag gagaagcggc tgaagtgctc gctttacctg aggcaaagcc tgtgatagaa
     2881 gccttcaagc aaattgtaaa agaaactcgc cccaccactc cggaaggttt ttccgaagca
     2941 atgaaacagg cgggagttca aacaggtgcg aaaggccgag c
//
LOCUS       DAOQJL010000491         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22412_length_2977_cov_4.852498, whole genome shotgun sequence.
ACCESSION   DAOQJL010000491 DAOQJL010000000
VERSION     DAOQJL010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22412_length_2977_cov_4.852498"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..292)
                     /locus_tag="PLG78_16755"
     CDS             complement(53..292)
                     /locus_tag="PLG78_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24825.1"
                     /translation="MPRYLQSEQIIPNKGMSYTMYEIEDVNTILRMLTWIPDADQISI
                     YPKPPVKQLFAPERCAQIDESQFLGLWQEGENRKK"
     gene            409..882
                     /locus_tag="PLG78_16760"
     CDS             409..882
                     /locus_tag="PLG78_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24826.1"
                     /translation="MDKGYILCVDDEVSVLETLREQLRLHFGQTHEVEVAASAEDALT
                     LIAQILESGGLIEVIITDQVMPGIKGDQFLEEVHKQLPDTIKILLTGHAGLASAIHAV
                     NKGGLNRYIEKPWNMDSLKEDISQLIHKFREHVENQRMVTMLERRVVELESRIGA"
     gene            809..1945
                     /locus_tag="PLG78_16765"
     CDS             809..1945
                     /locus_tag="PLG78_16765"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE24827.1"
                     /translation="MWKTREWSPCSKEESWNSNPESAHNVSGTVRALTAFILVTASFL
                     ISSSIFAESGKEEKGPTRVEALRAKILYASSTERRNEIRKVKSLAADEQKLLIPDVVK
                     LCTEDLDPNVRETSIRILTEMKTQEAEKAFLTALKDKVDDVQLAAISGLREIRSKEAG
                     PPLVELLKSLDVKKEQTKYSSIMRALGALHHKTDSAPLIKTAEEADTHEEIRRSIILY
                     LGDVKAQDGYEFLIKLARNEEKEPDLRAYAVNSIGRMKEARAVPDLKQILETIRNQKS
                     PKERARMSRLRFQSMVALMRLGDRSMVPEVMSAARDDDPVVRLRAVRQLGEMRTPEAK
                     ELLEFKSKYDENARVKKAAKLALDEWDKTPGDTGSDLDDSDKEK"
     gene            1942..2400
                     /locus_tag="PLG78_16770"
     CDS             1942..2400
                     /locus_tag="PLG78_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24828.1"
                     /translation="MIRSLLFTILFLGSIPGFAQQKEEVYEGDIRESDLRRRELVEKV
                     KKGEIPAAIKLRFSRKESDFLVFYDIEGRSIYYRYREDRFDTDAEKRVADLIAGEAYE
                     VTGPFLGMYYASMMHSSDHPKYKEMLNNADAIPAFLFKSARPLRIEQILL"
     gene            2448..>2977
                     /locus_tag="PLG78_16775"
     CDS             2448..>2977
                     /locus_tag="PLG78_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNE24829.1"
                     /translation="MAEFRNKDELLRGHAETAKQFQERWDQKGITGILDELEDLESLT
                     ARADFWDDPERAKKTSMKKSELEKRTQPWRDLRRDLDEFPDLVDLTLEEVKDQKAAIE
                     GLEGDLHKIRERLEDTLLAEALTGEDDGRDAIVTISSGAGGTESQDWTEMLLRMYLRW
                     FEKRGFRVETADIQQG"
BASE COUNT          860 a          735 c          749 g          633 t
ORIGIN      
        1 gcaacggatt gaaaatccgt gtgtcggcgg ttcaattccg tccctgggca atctacttct
       61 tgcggttttc gccttcctgc cacaatccta gaaactgaga ttcatcaatc tgtgcacagc
      121 gttccggcgc aaaaagctgc ttcacaggcg gtttgggata aattgaaatt tgatccgcgt
      181 ccggaatcca ggtgagcatt ctgagaatcg tgtttacatc ttctatctca tacatggtat
      241 aactcatacc tttgtttggg atgatttgtt cactctgtag ataacgaggc atacttcctt
      301 cggcctagac agcaagtacc tgcaaagtac aatatttttc ttgcacagga attccccttc
      361 gcgtttactc aagaaaatcg cggacgcgtc gatcttcagg gggagcaggt ggataagggc
      421 tatatattgt gcgtcgacga cgaagtgagt gttcttgaga ctctcagaga acagctcaga
      481 ctccatttcg gccaaacgca tgaagtagaa gttgcagcga gcgcggaaga cgcactcact
      541 ctcattgctc aaatcctgga atccggaggc ctcattgagg tcatcatcac agaccaggtg
      601 atgcccggaa tcaaagggga ccagtttctt gaagaagtgc acaagcaatt accggataca
      661 atcaagatcc tcctcacagg ccacgcaggt ctggccagcg ccatccatgc agtcaacaaa
      721 ggcgggctca atcgctacat tgaaaaaccg tggaacatgg actctcttaa agaagacata
      781 tctcaactca tccacaagtt cagagaacat gtggaaaacc agagaatggt caccatgctc
      841 gaaagaagag tcgtggaact cgaatccaga atcggcgcat aacgtttctg gaactgtccg
      901 ggccctcacc gcattcattc ttgtaaccgc atcctttctc atttcttctt ccatatttgc
      961 agaatcgggc aaggaagaaa aaggaccgac gcgcgtagag gcgcttcgtg cgaaaattct
     1021 ctacgcaagt tccaccgaac gcagaaatga aatccgtaag gtcaagagcc tggccgcgga
     1081 cgaacagaaa ctgttgatcc cggacgtagt caaactgtgc acggaagatc tagatccaaa
     1141 tgttcgcgaa accagcattc gcattctgac agaaatgaaa acgcaggaag cggaaaaagc
     1201 cttcctcacg gcgctgaagg acaaagtaga tgacgtgcag ctggctgcca tcagcggcct
     1261 tcgcgaaatt cgctccaaag aagcaggccc acctctggtt gaattgctga agagtctgga
     1321 cgttaagaaa gaacaaacca aatacagctc catcatgcga gcgttaggcg ctcttcatca
     1381 taaaacggac tctgctcctt tgatcaagac agcggaagaa gcagacactc acgaagaaat
     1441 tcgcagatcc atcatactgt atctggggga tgtaaaggca caggatggtt acgagttctt
     1501 aatcaagctc gcgcgaaacg aagaaaaaga gccagatctc cgggcgtatg cagtcaattc
     1561 catcgggcgc atgaaagaag cgcgagcagt tccggatctc aagcaaatac tcgaaaccat
     1621 tcgcaatcaa aaaagcccga aggaaagagc gcgcatgagc cgtcttcgtt tccagagcat
     1681 ggttgcactc atgcggctgg gagacaggtc catggtccca gaagtcatgt ccgccgcacg
     1741 cgatgatgat ccggtggttc gccttcgcgc tgtgagacag ctcggtgaga tgcgcactcc
     1801 ggaagcaaaa gaactcctcg aattcaaatc caaatatgat gaaaacgcgc gagtgaagaa
     1861 agccgcaaaa ctggcgctgg atgaatggga caaaactcca ggagacacag gatccgacct
     1921 ggacgattca gacaaagaaa aatgattcgc tcacttctgt tcacgattct atttctggga
     1981 agcattcctg gtttcgctca acaaaaagaa gaagtatatg agggagacat tcgcgagtcc
     2041 gatttgcgca ggcgcgaact tgtggaaaaa gtcaagaagg gtgaaatacc ggccgccatc
     2101 aagcttcgat tctcaagaaa agaatcggat ttcctggtct tctacgacat agaaggtcgt
     2161 tccatctact atcgctaccg cgaagatcgt tttgatacgg acgcggaaaa gagagtggca
     2221 gatctgattg caggagaagc ctacgaggtc acgggcccat ttcttggcat gtactacgcc
     2281 tccatgatgc actcatcgga tcatccaaag tacaaagaaa tgctgaacaa cgcagatgcg
     2341 atccccgctt tcctattcaa gtctgcacga ccccttcgaa tcgaacagat ccttttatag
     2401 aatcaaatag tacttgaaag cagcgctttt ttccagcttc ctggtttgtg gctgagttcc
     2461 gaaacaaaga tgagttgctg cgaggacatg cagaaaccgc aaagcaattt caggagcgct
     2521 gggaccagaa agggatcaca ggcatcctcg acgaattaga agatctggag tctctcacgg
     2581 cacgtgccga cttctgggat gacccggagc gtgcgaagaa gacttcgatg aagaagtcag
     2641 agcttgaaaa aagaacgcaa ccctggcgcg atcttcgccg cgatctggat gaattcccgg
     2701 acctcgtaga cctcactctg gaagaagtca aagatcaaaa agcggccatc gaaggactcg
     2761 aaggtgatct tcacaagatc agggaacgcc tcgaagacac gctcctcgca gaagcgttga
     2821 ccggcgaaga tgacggcaga gacgccattg ttacgatttc atccggagcc ggcggcacgg
     2881 aaagccagga ctggactgaa atgcttttgc gcatgtacct tcgctggttt gaaaagcgtg
     2941 gctttcgcgt agagacagca gacatacaac agggtga
//
LOCUS       DAOQJL010000492         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22682_length_2958_cov_3.413365, whole genome shotgun sequence.
ACCESSION   DAOQJL010000492 DAOQJL010000000
VERSION     DAOQJL010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22682_length_2958_cov_3.413365"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLG78_16780"
     CDS             complement(<1..1171)
                     /locus_tag="PLG78_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24830.1"
                     /translation="MAEEKIELTPDELKQVGTLLEPLTQAGRPLFPMEEKFKSDMTDL
                     DRIMADGGPGEEGELDLDAGESGLPDASAFPDGGEASPEEEGAESSFEFEPESTTSSD
                     SFADSGDLGSDSFSFEPGGEEPSAEVTEPATETPAETFDFGETDLATPEEPATPATDF
                     DFGSEEEAHADTDFGGGLSTETDPFGAESTESTGDAFAEQTDEPAAEAAESFDFGSPS
                     ETPATSDMDFGGETSSFSDFESSPSLGGDDAFGGGEATSSFDLTDSIAPDTDSFGGDS
                     LSSSDTGFDAGGADFSATSDFGSSDFSAGESAGSTPAPDAGVASFDATADESDLGDLT
                     TDLTGLSDLAPSDSDSDSGAFDTGDLSDMTRQANLSQGIGDEFTDEELAKIRARLD"
     gene            1234..2073
                     /locus_tag="PLG78_16785"
     CDS             1234..2073
                     /locus_tag="PLG78_16785"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24831.1"
                     /translation="MIRKAFLTAALSSIFCAPLVAEDWVGSYDSSVIAETPEDPVVYS
                     LETIEKLRRSVSPRYVRVLDWRSGQGKPGLVKGILFTCEALRSKDVAVAGDFSGWKPL
                     QMKRNRNGIFFYILPIREVEEGKRILSYRYKFQSAGIWFHDPRNPARIDDGLGGYVSH
                     FELEKEDVNRQAAFRVLREAHPGKERLVEFAVHEKALGRAAKRKSVTNVAIVGDFNGW
                     NPDHDWLKRDEDGIYRLRLRLPPGEYPYVLIVDGKWVLDPYNPQTRKHSRLDDLVSFA
                     IIQ"
     gene            2042..>2958
                     /locus_tag="PLG78_16790"
     CDS             2042..>2958
                     /locus_tag="PLG78_16790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE24832.1"
                     /translation="MIWFHLQSFNRFRFALLALFALASFWVIGADPHTPVLEPPLDIK
                     LRISGSFGEYRPASRYHHGVDIKTFEQNGIPVHAPLDGFVSRIHTSEYGYGNGLFFSS
                     GKYEFTFGHLNDFRGVRPDLEEYRLAVRILQPDHIAVVTPPPWFTFKKGDQMARTGES
                     GLGAPHLHFEVRENGTYRNPLAFSGMEIPDDTAPEILDVIAETPTARFKIPAIKNGAH
                     YDLASALPTGPVRLLIGCVDTQSALNRNGVAYLRFEVNGKEEYSRTVDSIEPQQLLLA
                     DGLYHPTLTIIGKVYYYYLYARAAEYPDRT"
BASE COUNT          695 a          750 c          780 g          733 t
ORIGIN      
        1 catccaggcg tgcccgaatt tttgcgagtt cttcatccgt gaattcgtct ccgattccct
       61 gcgacaggtt tgcctgacgc gtcatgtcgg agagatcacc tgtatcaaat gcgcctgaat
      121 ccgaatccga atcagaagga gccagatcag atagacctgt cagatcggtg gtaagatctc
      181 ctagatccga ctcgtctgca gtggcatcaa aagacgcaac tcctgcatct ggagcgggtg
      241 ttgagcccgc agattctcct gctgaaaaat cggaactgcc aaaatcagaa gttgcggaaa
      301 agtcagcgcc gccggcgtca aagcctgtat cagaagaaga aagagagtcg ccgccgaatg
      361 aatctgtgtc cggagcgatc gaatcggtca ggtcaaacga agaagtagct tctccgccgc
      421 caaacgcatc atcgccaccg agggaagggg aggattcaaa atcagagaaa gaagaagtct
      481 ccccgccaaa gtccatgtca gatgtggccg gtgtttcgga aggagagcca aaatcaaaag
      541 attcggccgc ctctgctgct ggctcatccg tttgttccgc gaacgcatcg ccagtagatt
      601 ctgtggattc tgccccgaat ggatccgttt cagtggaaag accgccgcca aaatctgtgt
      661 ctgcatgggc ttcttcttct gatccaaaat caaagtcagt cgctggagtg gctggttctt
      721 ccggagtcgc cagatctgtt tcgccaaaat cgaatgtctc tgcaggtgtt tcagtggcgg
      781 gttctgtgac ttctgcggat ggttcttctc cgcctggttc aaatgaaaac gagtcggatc
      841 ccagatcgcc tgaatctgca aatgaatctg aagaggtcgt ggactctggt tcaaattcaa
      901 agctggattc tgcgccttct tcttcggggc ttgcttcgcc tccatcagga aatgcagatg
      961 cgtccggcag gccggattcg cctgcgtcta gatccaattc cccttcttct ccgggtccgc
     1021 cgtctgccat gatgcggtcg aggtccgtca tgtcagactt gaacttctct tccatgggaa
     1081 agagaggtct gcccgcctgc gtaagcggtt cgagcagtgt gccaacctgc tttaactcat
     1141 ccggggtaag ttcgatcttt tcttcggcca cggtgctctg cttaaaggaa actactcagg
     1201 gaaacctatt tgattatcga cacttttagg gagatgatcc gcaaagcatt tcttacagct
     1261 gccctgtcct ccattttttg cgcgcctctg gtggccgagg actgggtcgg atcctacgat
     1321 tcgtccgtca ttgcagagac gcccgaagac cctgtagtct attctctcga aaccattgaa
     1381 aaactgcgta gatccgtgag tcccagatac gttcgtgtgc tcgattggcg ttcgggccag
     1441 ggcaaaccgg gcctcgtcaa ggggattctt tttacatgcg aagcccttcg ttcgaaggat
     1501 gtggccgtgg caggagactt cagtggctgg aagccattgc agatgaaacg caatcggaat
     1561 ggcattttct tctatattct gccaattcgc gaagtagaag agggaaagag aatcctatcg
     1621 tatcgataca agttccagtc tgcaggaatc tggtttcatg atccgcgaaa tccagcgcga
     1681 atcgacgatg gcctgggcgg ctacgtctcc catttcgagc tggagaaaga ggacgtaaat
     1741 cgtcaggcgg ccttccgagt tctgcgcgaa gcgcatcccg gaaaggagag gctggttgag
     1801 tttgcagttc atgagaaagc gctgggtcgg gccgcgaaga gaaagtctgt gacgaacgtg
     1861 gcgatcgttg gcgattttaa cggttggaac ccggatcatg actggctgaa gagagacgaa
     1921 gacggaatct acaggctgcg tctgaggctc cctcctggcg aatatcctta cgttctgatt
     1981 gtggacggca aatgggtgct tgatccttac aacccgcaga cacgcaaaca ttcgcgtctc
     2041 gatgatctgg tttcatttgc aatcattcaa tagattcaga ttcgcactgc tggctctgtt
     2101 tgcactggca tccttctggg tgattggtgc cgatcctcat acgcccgtac tcgagccccc
     2161 tctggatatc aagcttcgga tttcaggaag ttttggagag taccgtcctg cgagtcggta
     2221 tcatcatggt gtggacatca agacatttga gcagaatggg attccagtgc atgcaccgtt
     2281 agacggcttt gtttcaagaa ttcacacatc tgaatacgga tatggaaatg gattgttctt
     2341 ttcgagtgga aaatacgaat ttacgtttgg ccatctaaac gattttagag gcgttcgccc
     2401 ggaccttgaa gaatacaggc tggccgtgcg aattctacag ccagatcaca tagcagtggt
     2461 tacaccacct ccctggttca cattcaaaaa aggtgatcaa atggctcgca ctggcgaaag
     2521 cgggctcggc gcgcctcacc ttcattttga agtgcgcgaa aacggaacgt acagaaatcc
     2581 tctcgcattc tctggtatgg agattcccga cgatacggct ccagagattc tagacgtgat
     2641 tgcggagact cccactgctc gttttaaaat tccagccatc aagaacggtg cacactatga
     2701 tcttgcgtcc gctcttccga ccgggccagt gcgtcttttg attggatgcg ttgatacgca
     2761 gtctgcgctc aaccgaaacg gagttgccta tttacgtttt gaagtgaacg gcaaagaaga
     2821 atattccaga accgtggact cgatcgaacc ccaacagctg ctcctcgcag atggtctcta
     2881 tcatccgacc cttacgatca ttggaaaagt ttactattat tatctctatg ccagggcggc
     2941 ggaatatcca gatcgtac
//
LOCUS       DAOQJL010000493         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22770_length_2951_cov_4.580456, whole genome shotgun sequence.
ACCESSION   DAOQJL010000493 DAOQJL010000000
VERSION     DAOQJL010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22770_length_2951_cov_4.580456"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLG78_16795"
     CDS             complement(<1..617)
                     /locus_tag="PLG78_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE24833.1"
                     /translation="MKQSFGSALIHICLWLLFLLSIVFFAWNLSHGPANIVLSEAQIA
                     SLKVSLGLRIDGLSSLLFFMVTLLGASVGRYAARYLDGERRQRTFDWYLLLTIGCVSL
                     LVLSSNLLMLFAMWLATSYCLHQLLVYYRERPQAIIAARKKFIVSRIGDVCLLGGIAM
                     LYQMTGSFDFDEIFARKNELASTHGSNFHFAGVLFAAGAMTKSAQ"
     gene            complement(845..1597)
                     /locus_tag="PLG78_16800"
     CDS             complement(845..1597)
                     /locus_tag="PLG78_16800"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE24834.1"
                     /translation="MKIRLCQIQVDASAPEKNFSKIRQILTSASEDLIVFPEACTTGF
                     PYRRLTSITEANQTFLDEIALQSANRTGVILLPLLIEENNAIYNRQHWITGGKIAATY
                     DKIHLIGILGEDRFLTRGSAIVTVPWKPHIAGLATCYDLRFPELFRKLILKGADILTI
                     PAMWPIERAAHLHPLAKARAIENQAYVLVCNGCGPCGSMDLCGSSIAIDPKGEVIAQA
                     GSEEEQLTFEIDPAFAAEWRKDFPALGDIRLI"
     gene            complement(1594..1851)
                     /locus_tag="PLG78_16805"
     CDS             complement(1594..1851)
                     /locus_tag="PLG78_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNE24835.1"
                     /translation="MKRNTIVWLILIALTAASVTLSHAEFRRSYLIAGILGFALVKFL
                     GVAFQFMEIRTAHRGWKILLILFGVSFTVLAYAVSRGGVSL"
     gene            complement(1856..2521)
                     /locus_tag="PLG78_16810"
     CDS             complement(1856..2521)
                     /locus_tag="PLG78_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNE24836.1"
                     /translation="MKPVKHLIYRKCSWIKFYCARKGLFQDSTMDNLRKDFLYPPGGI
                     LIWIIISLEVVTFAAGLIAFLVTGQSEKELFALSRPLLDRNIGWINTIVLLTGGLFMA
                     NAIHSIREGKRKSSSLWMIAAGLAGITFLVLKCVEYSSKLKGGHDLGSDSFFTLYWLL
                     TGFHFLHVALATVILLVLASKVQKGKYSKKSHLDLETAGSFWHMCDLIWLFIFPVVYL
                     LPG"
     gene            2554..>2951
                     /locus_tag="PLG78_16815"
     CDS             2554..>2951
                     /locus_tag="PLG78_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24837.1"
                     /translation="MAKNFFWFVFALFVGTAIYTGWHPGFFDFNVRLGGLKLLVMLCF
                     AAFLGYSIYCSSRESLPATIQKMARLHWGRQIGIDLYIGLSIFLGIVFAMEGSPLAAA
                     AWILPTLLFGNLATLLYLSIHFDAIVSRLN"
BASE COUNT          752 a          709 c          752 g          738 t
ORIGIN      
        1 aattgcgccg atttggtcat ggcccctgcc gcaaaaagca ctcccgcaaa atggaagtta
       61 gacccgtgcg ttgaagcgag ttcatttttg cgcgcgaaaa tttcgtcaaa atcgaaagag
      121 ccagtcattt gatacagcat cgcaattccg cccaggagac aaacgtcgcc gattcgactc
      181 acaatgaact tttttcgcgc agctataatc gcctgtgggc gctcccggta gtagacaagg
      241 agttggtgaa ggcagtagct ggtggccagc cacatcgcga atagcatcaa gaggttgctc
      301 gacaaaacca ggagcgaaac gcagccaatt gtcaaaagca aataccagtc aaatgttcgc
      361 tgacggcgtt caccgtcaag atagcgcgcc gcataacgtc cgacactcgc accgagcagc
      421 gttaccatga aaaaaagaag cgaagaaagg ccatcgattc gaagccccag agaaactttt
      481 aggctggcta tttgagcttc actcaaaaca atattcgccg gcccatgtga aagattccat
      541 gcgaagaata cgatggacaa aagaaacaat aaccacaggc aaatatggat gagggcggat
      601 ccgaacgatt gcttcatgtg aacctccgct gcaccctgcc aaataatcat acgatctata
      661 tttcgtcaat tgtaaaccgt gtaaatttct acgtattttg tcttgccggg tctgctcatc
      721 tggcgcgtcc agaataaagg cctgttccgg gaaaaaagtt gatttgggcg tgcgttgtct
      781 atggacagtg tttggtcgcg acaatcgttt ggcactcaac ctgacgttgc gccaggctgt
      841 ttcttcaaat caaacgaatg tcgccgaggg ctggaaaatc ttttctccat tcagccgcga
      901 aagccgggtc gatttcgaac gttagttgct cttcttcgct ccccgcttgc gcaatcactt
      961 ccccttttgg atcaattgca atggagcttc cgcatagatc catggagccg cacggcccgc
     1021 atccgttgca aaccagtaca tatgcctggt tttcgatggc acgtgccttc gccagtggat
     1081 gcaaatgagc agctcgttcg atgggccaca tagctggaat ggtcaggatg tccgcccctt
     1141 tcaggatgag tttgcgaaag agttcgggaa agcgaaggtc gtagcaagtg gcaagtcctg
     1201 caatgtgggg tttccagggc acagttacga tggctgagcc acgagtgagg aatcgatctt
     1261 cccccagaat cccgatcaaa tggattttgt catacgtggc tgcgatcttg cctcctgtga
     1321 tccaatgctg acgattgtaa atggcgttgt tttcttctat aagaagaggc agcagaataa
     1381 ccccggtgcg gttcgcgctt tgaagtgcga tttcatctag aaaggtctgg tttgcctccg
     1441 tgatggacgt taggcggcga taaggaaagc ccgtcgtgca ggcctccgga aatacgatca
     1501 ggtcttcgct tgcggatgta aggatttgcc tgatctttga aaaattcttc tccggtgcgg
     1561 atgcgtcgac ttgtatctga cagaggcgaa ttttcatagg gagacgcccc ctcgggagac
     1621 agcgtatgcg agcacagtga aggatacgcc aaacagaatc aacaggattt tccagccgcg
     1681 atgagcggtg cgaatttcca tgaattggaa agcgactccc agaaatttta caagagcgaa
     1741 gcccagaata cccgcaatca aataggacct acgaaattcc gcatgcgaaa gcgtgacgct
     1801 ggcagccgtc agtgcgatta gaatcaacca gacaattgtg tttcttttca tgccattatc
     1861 ccgggagcaa gtagacgacc ggaaagatga acaaccagat gagatcgcac atatgccaga
     1921 acgaacctgc cgtttccaga tccagatgtg attttttaga atatttacct ttttgaactt
     1981 tgctggccag cactagaagt atgacagtgg ccagagccac atggagaaag tgaaaaccgg
     2041 tcagtagcca gtagagagtg aaaaaagagt ccgaccccag gtcgtgacct cccttcaact
     2101 tggaagagta ttctacgcac ttgagaacca gaaatgtgat tcccgcaaga cctgctgcta
     2161 tcatccagag actgctcgat ttgcgtttgc cttctctgat tgagtggatg gcgtttgcca
     2221 tgaagagtcc tccggtcaag agaacgatcg tgttgatcca tccaatgttg cgatccagca
     2281 gtggtcgcga aagtgcgaac agttccttct ccgattgacc ggttactaaa aacgcaatca
     2341 atccagcggc aaacgtgacc acttccaggg aaatgatgat ccatatgagg atgccacccg
     2401 gcggatagag gaaatctttt ctgagattgt ccatggttga atcttgaaag aggccctttc
     2461 gcgcacagta gaatttgatc catgagcact tgcgatagat caagtgcttg actggtttca
     2521 cgggccgcct caaaaaaggg agaggctcca agtatggcaa aaaatttctt ctggttcgtc
     2581 ttcgcgttgt ttgttgggac ggccatctac accggatggc atcctgggtt ctttgatttc
     2641 aatgtcagac tcggcggttt gaaactcctg gtaatgttgt gtttcgctgc cttccttggt
     2701 tattcgattt attgcagcag tcgagaaagt cttccggcca cgattcagaa gatggctcgt
     2761 ttgcactggg gcagacagat tggaattgat ctctatattg ggctttccat ttttttgggg
     2821 atcgtatttg ccatggaagg ttcacctctg gcggctgcgg cgtggattct acccactttg
     2881 ctctttggca atctggctac tctcctgtat ctctcgattc attttgacgc gatcgtttcg
     2941 cgattgaatc a
//
LOCUS       DAOQJL010000494         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22793_length_2950_cov_3.549568, whole genome shotgun sequence.
ACCESSION   DAOQJL010000494 DAOQJL010000000
VERSION     DAOQJL010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22793_length_2950_cov_3.549568"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..439)
                     /locus_tag="PLG78_16820"
     CDS             complement(53..439)
                     /locus_tag="PLG78_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24838.1"
                     /translation="MKRRNFRKARAVFQEGLELIPHPAYEWEAATWFFTAIADAAFLS
                     GDNQGAANYLQMARKCPDGLANPFILLRLGQALTNLGEKEEGVGFLLEAFMVGGHDVF
                     LGEHPRYWNLIQPMVEVIKHGATSRD"
     gene            complement(599..1942)
                     /locus_tag="PLG78_16825"
     CDS             complement(599..1942)
                     /locus_tag="PLG78_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE24839.1"
                     /translation="MKKVPVLAGSWPIVGHLFQFRKNPLAFILDAKQKHGDIIRLNLA
                     GKPVYILSDPALIHEVLVVQAKSFHKSRALKLAKMLLGEGLLTSEGEHHLRQRRMMAP
                     AFHRKKIMDYGLVMSGEAHKFSGNLQAGANINLHDAMMQLTLAIVARTLFDADVAGDA
                     REVGSALHDALSLFERVSNPFAPLLAKLPLPATRKLNRARERLFRVIDRIIREHEGVD
                     RGDLLSMLLAAVDEEDNSKMSLEQVRDEALTLFLAGHETTANALTWAFYLLAKHPQVR
                     HKLQEEADRVAKDGAPISPEQIASLPYARDVFQESLRLYPPAWLIGRSAMADVTLGEW
                     DIAQGSIILMSQFAVHRDERWFAEPLSFRPERWQEPSDRPKFAYFPFGGGPRTCIGDQ
                     FAWMEGTLLLAEISRHWEFEPLEETLEPEPLITLRPRGGLPVKCVARNGNVARQQ"
     gene            complement(1939..>2950)
                     /locus_tag="PLG78_16830"
     CDS             complement(1939..>2950)
                     /locus_tag="PLG78_16830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE24840.1"
                     /translation="DRGDIETRVRAVRFGGNAYFEKPADAGALIRAIDNTVHKKEIEL
                     YRILIVQQETETAARYAEILNSAGMMTEILTDPMRLFFSLQDFRPDLILMNLYYPDCL
                     GMEIAAVVRQEEANQSIPIIYLTEETQMQRILPALNKGGDDFLPLPVTPDRLISSVAA
                     RVDRSRILRSLLVRDSLTGLLNHTAVREQLDDLVQRAKRKNETFSFGMVDIDKFKRVN
                     DEYGHPVGDRVIQSLARTIRQRIRRVDAAGRYGGEEFAVIFDGLDTDAAVRALDRIRR
                     AFSQIRHHSDKGDFFSTFSSGVVSFPDFQTGTELNDAADRALYAAKRSGGNCVARIDR
                     KK"
BASE COUNT          686 a          797 c          730 g          737 t
ORIGIN      
        1 gatcccacct ggggtatgaa aatcccacca aagcttgcgg attttgtctc cctcagtccc
       61 tggaagttgc gccatgcttg atcacctcca ccataggctg gattagattc caataacggg
      121 gatgttcccc gagaaacacg tcatgtccac cgaccatgaa tgcttcaagc aaaaacccga
      181 caccctcctc tttctcacct agattcgtca gcgcctggcc cagacgcagc aagatgaagg
      241 gattagcaag cccatctgga catttacggg ccatttgcag atagttcgcg gctccctggt
      301 tatcgcccga aagaaaggct gcgtcggcaa ttgccgtaaa aaaccacgtg gcggcttccc
      361 attcgtaagc cggatgcggt atcagctcaa gcccttcctg aaagactgca cgtgccttgc
      421 gaaaattgcg tcttttcatg tgtagattcc cacgccgcgc aattgcgtcg agcgcggtag
      481 ctatttcatc tgacaattgt gccaagcaaa tgctccattt cttgtatttc tatcgtctat
      541 cgagtgggtg gcaaatacgt ctttcattct atgccggcta acgtaaggga caactcgatc
      601 attgttgtct ggccacattg ccatttcgcg ctacgcattt cacaggcaat ccgccgcgcg
      661 ggcgaagagt gatgaggggc tccggttcca gtgtctcttc gagcggctca aattcccagt
      721 ggcgcgaaat ttcggccaga agcagagtcc cttccatcca cgcgaattga tctccaatgc
      781 aagtgcgcgg acctcctcca aaaggaaagt acgcgaactt aggacgatcg gatggttcct
      841 gccagcgctc cgggcgaaaa gagagaggct ctgcaaacca tctttcgtct cgatgcactg
      901 caaattgcga catcagaata atggatcctt gcgcgatatc ccattctccc agagtcacat
      961 ccgccatggc agagcggcca atcaaccacg caggtggata caggcgaagg ctttcctgga
     1021 atacatcccg agcgtagggt agacttgcga tttgttcggg tgaaatgggc gcgccatctt
     1081 ttgcgactct gtctgcttct tcctgcaatt tatgcctcac ctgaggatgt tttgcaagaa
     1141 ggtagaaggc ccaggtcaat gcattggcag tggtctcatg gccggcgaga aagagagtga
     1201 gggcttcatc gcgcacctgt tccagactca tcttgctatt gtcctcttca tccactgcgg
     1261 ccagaagcat agaaagcaaa tcaccgcggt ccactccttc gtgttctcgg atgattcgat
     1321 caatgacgcg gaacaatctt tctctggcgc ggttgagttt ccttgtggca ggaagtggaa
     1381 gcttcgcgag caagggagca aatggattgg acactctttc aaaaagactc aatgcatcgt
     1441 ggagtgcgct tcccacttcg cgcgcatctc ctgccacatc cgcatcaaac agagtgcgcg
     1501 cgacgatcgc cagagtcagc tgcatcatcg catcatgtag attgatattt gctccagcct
     1561 ggagattgcc gctaaacttg tgcgcttcgc ctgacatgac caggccgtag tccatgatct
     1621 tctttctgtg aaaggctggc gccatcattc gcctctgtcg aagatgatgc tctccctctg
     1681 acgtaagaag gccttctccc aggagcattt tcgcgagctt gagcgctctg ctcttgtgaa
     1741 agcttttggc ctggactaca agcacttcat ggatgagtgc tggatcggaa aggatgtata
     1801 ctggttttcc agcgaggttg aggcgaatga tgtctccatg tttttgcttc gcgtcgagta
     1861 taaacgcaag cgggttcttt ctgaattgaa atagatgccc gacaattggc catgagcccg
     1921 cgagaacggg aaccttcttc atttcttcct gtcaatgcgc gccacgcagt tgcccccgga
     1981 tcttttggcg gcatacagcg cacgatccgc cgcatcgttg agttctgttc cagtctggaa
     2041 atcagggaaa ctcacaactc cgcttgaaaa cgtggagaag aaatctcctt tatctgaatg
     2101 atggcgaatc tgcgaaaagg cacggcgaat tcgatcgagt gcgcgaacag ccgcgtctgt
     2161 atccagacca tcaaagatga cagcaaattc ttctccgccg tatctccctg ccgcgtctac
     2221 cctgcgtatt ctctggcgga tggtgcgtgc cagactctgt atgactctgt ccccgacagg
     2281 gtgaccgtat tcatcattga ctctcttgaa cttgtcaatg tccaccatac caaaagagaa
     2341 cgtttcattc ttcctttttg cgcgctggac tagatcgtct aactgctccc gcacagcagt
     2401 gtgattgaga agtccggtca gactatcgcg aaccagaaga gaacgtaaaa tgcgcgagcg
     2461 gtccaccctg gccgccacag aagaaatcaa acgatccgga gtcacaggaa gaggtagaaa
     2521 atcgtctcca cctttgttga gtgcaggcag aatcctctgc atttgagttt cttccgtaag
     2581 ataaatgatt ggaatgctct ggtttgcttc ttcctggcga accaccgctg cgatctccat
     2641 tcccagacaa tctggatagt aaagattcat aagtattaag tccggcctga aatcctgcag
     2701 agaaaagaac agccgcattg gatctgtgag tatttccgtc atcattcccg cggaattgag
     2761 gatctctgcg tatcttgccg cagtctccgt ttcctgttga acaatgagaa tgcgatagag
     2821 ctcgatttct tttttgtgaa cggtattgtc gatggcgcga atgagcgcac ctgcatctgc
     2881 cggtttttca aagtatgcgt ttccaccaaa tcgaaccgcg cgcactcgag tttcaatgtc
     2941 tccgcgatcc
//
LOCUS       DAOQJL010000495         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_22852_length_2945_cov_4.829758, whole genome shotgun sequence.
ACCESSION   DAOQJL010000495 DAOQJL010000000
VERSION     DAOQJL010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22852_length_2945_cov_4.829758"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG78_16835"
     CDS             <1..237
                     /locus_tag="PLG78_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24841.1"
                     /translation="LADPQVPEEVKAELRKTRAQLEENMAKNKPWAEGVMKGASILSD
                     KETLEVIQRNREKIRSAYTGGIPEPAGEFMGRKL"
     gene            complement(234..947)
                     /locus_tag="PLG78_16840"
     CDS             complement(234..947)
                     /locus_tag="PLG78_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24842.1"
                     /translation="MMTEKMESIQKPGRIESSYRMETVEGLELWRLGRDRFSDFNQFV
                     YGVYSEAFGENGKIPFEKRDIEAASDEHFDHARVCAVIHPDGTLLGTWGLILKERSDS
                     ALLPIEKAFSLTTEEIISKMNVTEVRYIFNGWRTAVDKNALLDHGFAANRSMFVFDLL
                     LRGLTEDFGKPEWFLGVAEMEMLVLRYHKRVGLPWHVLGDPIHYWGRDRFPCALSLVE
                     FRETLRVHHPDRFRFFYER"
     gene            1017..1613
                     /locus_tag="PLG78_16845"
     CDS             1017..1613
                     /locus_tag="PLG78_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE24843.1"
                     /translation="MKTQSKKRKVPYGMAREDIAAAARDLFYEQGLATGLDEILKRAD
                     VSKPTFYHHFGSKEELESQYLTAQAEFLRTSVDRIAGKSRDACQFIRRWMEFIRRRAS
                     SPTFTGCPLGNFAIEAKSAHNARVLREFGLSIEKLEDTFKAKGIQATRARELAPLIFM
                     IYEGALLMCSATEERSHLSRATELMVRLVREKKSPTLK"
     gene            complement(1705..1950)
                     /locus_tag="PLG78_16850"
     CDS             complement(1705..1950)
                     /locus_tag="PLG78_16850"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNE24844.1"
                     /translation="MLNLIWAIVVGFFVGLLARFLLPGNDKMGFILTTICGVVGGVVG
                     TYIAPYIPFLPGGTIGSLIASTIGAIIILVVLRIVRR"
     gene            2082..>2945
                     /locus_tag="PLG78_16855"
     CDS             2082..>2945
                     /locus_tag="PLG78_16855"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE24845.1"
                     /translation="MMARLSSIALGALLLIACRSPVDCRPADLSCNGLASLLLYSRVS
                     LVRAAYLTDNVSNLLYSYSIDPTTGQPSLIGSIGTQVLPRGVAVHPAGYVYAANETGG
                     SISIYRILPGYSVSLVTHFNVGGGTPSALAINSAGTLLYVTRQGVSLVNSYWIDASTG
                     LLSSTGFTGTTDATTNQISLHPTLPYAFTGGTGATSYGFLLGANGSFTTIPGAVTATD
                     TFYPLITPNGRYLLISDNGQSRVELFSVSQQNGALTALSPAFFSVGAAAGVSFVDASG
                     TSLYVCIPISNS"
BASE COUNT          761 a          774 c          717 g          693 t
ORIGIN      
        1 ctggcagacc ctcaagtgcc cgaagaagtc aaagccgagc tacgaaagac cagagcgcag
       61 ctcgaagaaa acatggcaaa gaacaaacca tgggcagaag gcgtcatgaa gggcgcaagc
      121 atcctcagcg acaaagagac gctggaggtg atccagcgaa atcgtgaaaa gatccgttcc
      181 gcctacaccg gcggaatccc ggaacctgcc ggagaattca tgggtagaaa actctaacgc
      241 tcgtaaaaaa aacggaagcg atccgggtga tgaacgcgaa gagtctcgcg aaactcgacg
      301 agggaaagag cacacggaaa gcgatcgcgc ccccagtagt gaatgggatc ccccagaaca
      361 tgccagggta gcccaactcg tttgtggtag cgcagcacaa gcatttccat ctccgccaca
      421 cccagaaacc actccggttt tccaaaatct tcggtgagac cgcgaaggag aagatcgaat
      481 acaaacatag accgatttgc ggcaaaccca tgatccagga gcgcattttt gtccaccgct
      541 gtgcgccagc cattgaagat gtagcgaact tcggtgacat tcattttaga aatgatctct
      601 tccgtagtca gtgagaatgc cttttcaatc ggcaagaggg cagaatcgct tctttctttt
      661 agaatcaggc cccaggttcc aaggagagtt ccatcaggat gaatgacagc gcacactctg
      721 gcatgatcaa agtgttcatc gcttgctgcc tctatgtctc ttttctcaaa cggaatcttg
      781 ccgttttcac caaatgcttc gctgtaaacc ccgtatacaa actggttgaa gtcactaaag
      841 cgatctcttc ccagtcgcca tagttcgagc ccctcaaccg tctccatcct gtacgaagac
      901 tcaattctac cgggcttttg tatgctttcc attttctcag tcatcattgc ttttctcctg
      961 aaatctggtt gattttttta tccaaagact attactgacc agtaagtatt atttgcatga
     1021 agacgcaatc caaaaaacgc aaggttccgt acggaatggc cagagaagac atagcggccg
     1081 cggccagaga tctattctat gagcagggac tcgccacagg cctcgatgaa atcctcaaac
     1141 gcgctgacgt aagcaaaccc accttctatc atcactttgg aagcaaagag gagctggaat
     1201 ctcagtacct gactgcccag gcagagtttc tgcgcacttc ggtagatcgc attgcgggta
     1261 aatccaggga cgcttgtcag ttcattcgca gatggatgga attcatccgc agaagagcca
     1321 gttcccccac tttcacagga tgccctcttg gcaattttgc catcgaagca aaatccgcac
     1381 acaatgcgag ggttctgcgc gagtttggtc tttctattga aaagctagaa gacacgttca
     1441 aagcaaaagg gatccaggcg acgcgtgctc gagagctggc gccgttgatt tttatgatct
     1501 atgaaggtgc cctgctcatg tgttcggcta cggaagaacg atctcacttg agccgggcta
     1561 ctgaattgat ggtgagactt gtgagagaaa aaaaatcacc aactttgaaa tgataaaaga
     1621 cagccggcga atgatcgcct aaccattgaa aggtggtatt gtgaaggact gttgcgacca
     1681 gccataggcc ggccgcaaga tcgtttacct gcgaacgatg cgcaggacaa cgagaatgat
     1741 gatcgcccca atcgtagacg cgatcaggct tccgatagtc ccacctggaa gaaacggaat
     1801 gtaaggcgcg atgtacgtgc ccacaacacc accaacgaca ccgcaaatcg ttgtgagtat
     1861 gaagcccatc ttgtcatttc ctggaaggag gaaacgagcc agtagaccga caaagaaacc
     1921 aacgacgatt gcccagatca gatttaacat ttatttagct cctgaacctt tttgcatgta
     1981 tttgagctcc tttgtgaatt ctgtcaagtg cttgcatgga agttaactgc cgatttgtgt
     2041 gtaagagagg cgggctttgg atcgtctaat tcaattggaa aatgatggcc cgtctgtcct
     2101 ccatcgccct gggcgccctg cttctgatcg cctgtaggag ccccgtggat tgtcggccgg
     2161 cagacttgag ctgcaatggc cttgcatcct tgttgcttta ctctcgagtc tcactggtca
     2221 gggccgcgta cctcacagac aacgtgtcca accttctgta ttcctattcc attgatccga
     2281 ctacaggcca gccttccctg atcggctcga ttggaaccca ggtcctgccc agaggtgttg
     2341 cggttcatcc agcaggatat gtatatgctg ccaacgaaac aggaggcagc atatcgattt
     2401 acagaatctt acctggctat tcggtcagcc tggttacgca cttcaatgta ggtggtggaa
     2461 ctccatccgc cctggccatc aactcggcag gcactctcct ctacgtgacc agacaggggg
     2521 tctcgttggt gaacagctac tggatcgatg catctaccgg acttctgtct tccaccggtt
     2581 ttacgggcac gacagacgcg accaccaatc aaatttcgct tcatcctaca ttgccttatg
     2641 cattcacagg aggaacggga gctacaagct atgggtttct actcggcgca aatggctctt
     2701 ttacgacgat ccctggcgca gtaacggcca cagatacgtt ctatcctctg attacgccaa
     2761 acggacgtta tctgctcatc tcagacaatg gacaaagccg cgttgaactg ttttctgtat
     2821 cgcagcagaa cggagccctc accgcacttt cgcctgcatt ctttagtgtg ggggctgctg
     2881 ctggagtttc attcgtcgat gccagcggaa caagcctgta tgtttgcatt cccatttcga
     2941 attcg
//
LOCUS       DAOQJL010000496         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23016_length_2932_cov_3.841154, whole genome shotgun sequence.
ACCESSION   DAOQJL010000496 DAOQJL010000000
VERSION     DAOQJL010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23016_length_2932_cov_3.841154"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLG78_16860"
     CDS             complement(<1..277)
                     /locus_tag="PLG78_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24846.1"
                     /translation="MPELSGQVKVFRAHFTAKKLVLQTQSISIARMQELLPLSAEVRD
                     EPVTASQRLSEFQFAPAGTDPGLALRFLDAFRVRLSHSFDHLSSLSNS"
     gene            287..1078
                     /locus_tag="PLG78_16865"
     CDS             287..1078
                     /locus_tag="PLG78_16865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE24847.1"
                     /translation="MRSSDFKTILITGASSGIGLELARVCAEKGHDLVLIGRHAGRLK
                     DAATELAAHGVKVHTIRKDLSEPEAADAVLKELTRKKISVDALVNNAGFGDHGAFEKS
                     DWEKVSMMIMTNVHALTHLTRLVLPSMRAHGRGIIVNIGSTAGLQPGPYMAVYYATKA
                     FVNHFSEALDYELRGSGIRVTCILPGATSSGFQEAASLNGSLIDRLAKLATSRQVAEF
                     TYAKMMAGRRFAIHGVFNRILAFSLRLTPRFLVTFMAAKINGEGS"
     gene            1250..1864
                     /locus_tag="PLG78_16870"
     CDS             1250..1864
                     /locus_tag="PLG78_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24848.1"
                     /translation="MMNKTKHSFLLILGALFAFSIQCKSAANLRATDTLDKADGVLIF
                     SMRYFKVQNATKIEEPLGSFYREGMRARSGNSTYFVYDGTTLFLSDGSERRANISHPF
                     LLKFAAGDIVIQSIRSTGRYPVSGGTMNLTKLAPVGVRMKVRPQAITYGGEITITEDY
                     ATKPTTFRCEIASEMSTDQIKDALSGFQNTSGFEIKEELGTCPN"
     gene            complement(1920..2240)
                     /locus_tag="PLG78_16875"
     CDS             complement(1920..2240)
                     /locus_tag="PLG78_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24849.1"
                     /translation="MIKKTIGIIALFAAAYGVFWITSKRHELRNFPGIISSFYSKEMC
                     SCLFVAGQTEEFCNNYARQWVPIQGSPVVDRERKTVRVTGLFRTNEAVYTGEKTGCVL
                     RSGD"
     gene            complement(2250..2693)
                     /locus_tag="PLG78_16880"
     CDS             complement(2250..2693)
                     /locus_tag="PLG78_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24850.1"
                     /translation="MIRRHSLHMYAPIAFALLWTSCAIDGSDVVSGGEMIDRIRTAVS
                     IKVGICYPGDASNVLLAKEVTTRFDRGQFYSTKDVDRCIQDFDALPCAFFYQNFGRQS
                     VSLFVGNVYAFVCPNLSDKEFFSPTYMQGNFPESSSTKHSSTSSN"
     gene            complement(2690..>2932)
                     /locus_tag="PLG78_16885"
     CDS             complement(2690..>2932)
                     /locus_tag="PLG78_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNE24851.1"
                     /translation="WDGNYGAQWWQNGDAPGLKAPWPDAPMDTYVASGHWGQRIYVIP
                     SLDIVAVRVGDDRRHPPEGGFSDNEFLKWIAQAAKK"
BASE COUNT          743 a          730 c          749 g          710 t
ORIGIN      
        1 gggagttgga gagactggag agatgatcga atgaatgact gagacgaaca cgaaatgcgt
       61 ccaaaaatcg cagggccaat ccggggtccg tgcctgcagg tgcaaactgg aactcggaaa
      121 ggcgctggga tgctgtgacc ggttcgtccc gtacctccgc agagagcggc aaaagttcct
      181 gcattcgagc aatgctgatc gattgcgtct ggagtacaag tttttttgcc gtgaagtgtg
      241 cgcgaaacac tttgacctgt cccgatagtt ccggcacaac gcctccatgc gatcatcaga
      301 ctttaaaact atcctgatca ccggggcttc tagcggaatt ggcctcgaac tcgcaagagt
      361 ttgtgcagag aaaggccacg atctggttct cattgggagg cacgcaggca gactcaaaga
      421 tgcggccaca gagctggccg cgcacggagt aaaagtccat acaattagga aggatctgtc
      481 ggagccggag gctgcggacg cagtcttgaa agaactgact cgcaaaaaga tctcagtgga
      541 tgcgctcgta aacaatgcag gattcggaga ccacggcgca tttgagaaat cagattggga
      601 aaaagtgtcc atgatgatca tgaccaacgt gcacgctctc actcatctga ctcgcctggt
      661 gcttccatcc atgcgcgctc acggtcgcgg aatcattgtg aatattggat caacagccgg
      721 actccagccc ggtccataca tggccgttta ctacgcgacc aaagcattcg tgaatcattt
      781 ttctgaagca ctggactatg aactgcgcgg ttccgggatt cgcgtgactt gcatcttgcc
      841 cggagccacg agctccggat ttcaggaagc agcatcactc aacggaagct tgattgatcg
      901 acttgcgaaa ctcgcgacgt cacgtcaggt tgctgaattc acgtacgcga aaatgatggc
      961 gggaagacgc tttgcgattc acggagtgtt caatcgcatc cttgcgtttt ctctgaggct
     1021 cactccgcgt tttctggtta cattcatggc cgcgaagatc aacggagaag gtagctgaac
     1081 aggcttcgtg tcagcaaaaa agagtcttga agggcttcgg agaccggcat cacactcgac
     1141 cggaggtcca gatattttcg gaatctggat ttcagattgc ccgaaatcat gatgctgttg
     1201 actttgagtt aaaaccaaac ttaatactaa ggttttttga ggtttcagca tgatgaacaa
     1261 aacaaagcat tccttccttc tgattctggg cgcgttattc gctttctcta ttcaatgcaa
     1321 atcggctgca aacctgcgtg cgacggatac gcttgacaaa gcggacggag tgctgatttt
     1381 ttcgatgcgc tatttcaagg tgcaaaacgc aacgaaaata gaagaacctc tcgggtcatt
     1441 ttatcgtgag ggtatgagag cacgttccgg gaactccaca tattttgtat atgacggaac
     1501 cactcttttc ctgagcgacg gttccgaacg ccgcgccaac atcagtcacc ctttcttgct
     1561 caagtttgca gcgggtgata ttgtcattca gagcatccgg tctactggcc gttatccggt
     1621 ttccggcggg actatgaatt tgacaaagct cgccccggtt ggagttcgaa tgaaagtgcg
     1681 cccccaggca attacttatg gtggagagat cacaatcacc gaagactacg ctacgaaacc
     1741 aaccacattt cgatgcgaga ttgccagtga aatgagcaca gatcagatca aggatgcatt
     1801 gtcaggattc cagaacacgt ctgggtttga gattaaggaa gagctaggta cgtgcccaaa
     1861 ctgaaaagat aactactcag tgttcatggc cgcgaagatc aacggagagg tcgttcggat
     1921 cagtcaccgg accgaagaac gcagcctgtt ttttctccag tgtagacggc ctcattggtt
     1981 cgaaacagcc ctgtaacgcg cactgtcttt ctttctctgt ccacaacagg cgatccctgg
     2041 atgggcaccc actggcgcgc atagttgttg cagaattcct ctgtctgtcc tgccacgaat
     2101 aaacaggagc acatttcttt gctgtagaaa gaagaaatga ttcccggaaa gttgcggagt
     2161 tcatgtcgct tgcttgtgat ccagaatacg ccgtaagcgg cggcaaacag tgcgatgatt
     2221 cctatagttt tcttaatcat tatatttcct caattacttg aagtgctgct gtgtttggtg
     2281 ctagatgatt ccgggaaatt tccctgcata tacgttgggc taaagaactc cttgtcgctc
     2341 aagtttggac agacaaatgc atacacgttc cctacaaaga gggaaacgga ttgccttcca
     2401 aagttctgat aaaagaaagc gcacggaagg gcatcaaaat cctgaatgca cctgtccacg
     2461 tctttggtgg agtaaaactg gccgcgatca aagcgagtcg tgacttcttt tgcaaggagc
     2521 acattgcttg catctcccgg atagcagatc ccaactttga tgctcactgc tgtgcgaatg
     2581 cgatcaatca tctctccgcc ggaaacaaca tcagatccat caatcgcgca ggaagtccag
     2641 aggagtgcga aagcaatcgg cgcgtacatg tgcagagaat gtctgcggat catttctttg
     2701 cggcctgcgc aatccattta agaaactcat tgtctgagaa tccgccctct ggtggatgtc
     2761 ggcgatcatc tccaacgcgc actgcgacta tatccagact tggaatcacg tagattctct
     2821 gcccccagtg tccgctcgcc acataggtgt ccataggtgc gtctggccat ggagccttca
     2881 ggccaggtgc atctccattt tgccaccact gtgctccgta gttgccatcc ca
//
LOCUS       DAOQJL010000497         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23025_length_2931_cov_5.036161, whole genome shotgun sequence.
ACCESSION   DAOQJL010000497 DAOQJL010000000
VERSION     DAOQJL010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23025_length_2931_cov_5.036161"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLG78_16890"
     CDS             complement(<1..240)
                     /locus_tag="PLG78_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24852.1"
                     /translation="MARVLLAASLTFFTTGLFSKEPIFPPGLKESEFKRMQEFYRALD
                     AGLVPTLDAVAEETKAEPPTRNLTDADEKSIQEIFP"
     gene            complement(246..956)
                     /locus_tag="PLG78_16895"
     CDS             complement(246..956)
                     /locus_tag="PLG78_16895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE24853.1"
                     /translation="MKKLIAILLTASSFLALHAEEKALALVVQTAGKSDLVRDGKTLG
                     VKANDLVRKGDLLRTSKDASMSVQLSTGAIFRMGPDTEVALQDLVRDGTGFHVKLDVK
                     KGQLASKLDQLSKKDTYQVQTPTAVAGVRGTSFIVEISSAVQSAEVLVSDGAVAVTDN
                     KTGTEQVCPSGSKIVADGKAMIQSLVDEHEKEKFRIFADMKKFQRENFEQLIEQQKRN
                     QEMMRQQREQMNMDSVRP"
     gene            1041..>2931
                     /gene="acs"
                     /locus_tag="PLG78_16900"
     CDS             1041..>2931
                     /gene="acs"
                     /locus_tag="PLG78_16900"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNE24854.1"
                     /translation="MTMERIVQPFADFAAQANVKKSEYEALYKESVENPEAFWSRMAD
                     RLTWFKKWDKVLEHDFANAKSKWFTGGKLNVSYNCLDRHLSSTLKNKAAIIWEGDNPE
                     ESKTFTYQELHREVSRVGNVLRKRGVKKGDRVLIYMPMIPELAISALACTRIGAVHSI
                     VFGGFSPESLVGRIEDCKPVMIITSDAGYRGGKTIDMKRNIDAALEKSAQKVKDVIVV
                     RRVGQEQFYKWYEDRDHWWHDLLRDPSITAVCEPEQMDAEDPMFILYTSGSTGKPKGV
                     LHTTAGYLLGASLSHFYVFDHKPEDTYWCTADIGWVTGHSYILYGPLSNGATSIMFEG
                     VPQYPDWGRFWDVVDKYQVNVFYTAPTAIRALAKEGPAHVEKRSLKSLRLLGSVGEPI
                     NPEAWEWYFKHVGKEKCPIVDTYWQTETGSIIISPLPVFALKPGSATLPFFGIRPVLL
                     DSDGKKIEGEGSGNLAIEFPWPSIMRGVYGDPDRFHQTYFTQFKGYYFTGDGARRDKD
                     GYYWVTGRVDDVLNVSGHRIGSAEVEHAIVEHRAVAESAVVGYPHDIKGQGIYAYVTL
                     KNGIEASEELKHEILNTVSEKIGKIARPDIIHWAPGLPKTRSGKIMRRILRKVAEFEF
                     GSLGDI"
BASE COUNT          750 a          728 c          740 g          713 t
ORIGIN      
        1 gggaaatatt tcctgaatcg atttctcatc tgcatccgtc aggtttcttg tgggaggttc
       61 tgcttttgtt tcttccgcga cagcatcgag ggtgggtaca agccctgcat ccagcgcgcg
      121 gtagaactcc tgcattctct tgaactcgga ctctttgagt ccaggaggga aaatgggctc
      181 tttggaaaag agcccggttg taaaaaatgt tagactggct gcgagtaaaa ctcgggccaa
      241 tctcctcatg ggcgaaccga gtccatgttc atctgctcac gctgctgacg catcatttcc
      301 tgattgcgct tctgctgttc gatgagctgt tcaaaatttt cgcgctgaaa ctttttcata
      361 tctgcgaaga ttctgaattt ctctttctca tgctcgtcca cgagactctg gatcattgct
      421 tttccatccg ctacaatctt gcttccgctg ggacaaactt gctctgtgcc cgtcttgttg
      481 tctgtgacgg caacggcgcc atcacttacc agaacctctg cactctgcac tgcagatgaa
      541 atttcaacta tgaaagaagt gcctcgaaca ccggccacag cagtgggagt ctgcacctgg
      601 taagtgtctt tcttgctcaa ctgatcgagc ttggatgcaa gctggccttt cttcacatcc
      661 agctttacat gaaatcctgt gccatcgcgc acgagatcct ggagggccac ttctgtgtcc
      721 ggccccatgc gaaagatcgc gcctgtagaa agctgcacgg acatgcttgc atctttggat
      781 gtgcgaagaa ggtccccttt acgaaccaga tcgttggctt ttacgcccag agttttgccg
      841 tctcgcacca ggtctgattt gcctgccgtt tgaacgacaa gagcgagtgc tttttcttcc
      901 gcgtgcagag caaggaagct cgaagcggtc aataggattg caattagttt cttcacgaga
      961 tacctcttag gaaggacggg gcagactgtc gaatggttcg atttttcttg acccaatccg
     1021 cctcgcaatc gaaatccagc attactatgg aaagaatcgt tcagcctttc gcggattttg
     1081 cggcccaggc aaatgttaag aaatcagaat atgaagctct atacaaggaa tctgtagaaa
     1141 acccggaagc attctggtcg cgcatggcgg atcgtctgac ctggttcaag aaatgggata
     1201 aagtcctgga acatgatttc gcaaacgcga aaagcaaatg gttcacgggc gggaagctga
     1261 acgtttctta caactgtctg gacagacact tgagttcgac tctcaagaac aaagccgcca
     1321 tcatctggga aggggacaat ccggaagaat ccaaaacgtt tacgtaccag gaacttcatc
     1381 gcgaagtcag ccgcgtagga aacgtattga gaaaacgcgg agtaaagaaa ggagatcgag
     1441 tcttaatcta catgcccatg attccagagc tggccatcag cgccctggca tgcacgcgga
     1501 tcggtgcagt tcactccatt gtattcggcg gcttttcccc tgagtcgctc gttggcagaa
     1561 tcgaagattg caaaccagtc atgatcatca cttcagacgc tggctaccgc ggtggaaaga
     1621 ccattgacat gaagcgcaac atagacgcgg cactcgaaaa gtcggcccag aaagtgaaag
     1681 atgtaatcgt tgttaggcga gtgggacaag aacaattcta caaatggtat gaagatcgcg
     1741 accactggtg gcatgacctt cttcgtgacc cttcgatcac tgcagtttgc gaaccggagc
     1801 aaatggacgc agaagatcca atgttcatcc tgtacacttc cggctccaca ggaaaaccaa
     1861 aaggagtctt gcataccact gcgggctact tgcttggcgc aagcctcagc catttctacg
     1921 tgttcgatca caaaccagaa gatacatact ggtgcacagc agacattgga tgggtcaccg
     1981 gacatagcta cattctatac ggaccgcttt caaatggtgc cacttccatc atgtttgaag
     2041 gtgtaccaca atatccagac tggggtcgat tctgggacgt agtagataaa taccaggtga
     2101 atgttttcta cactgcgccc actgccattc gcgctcttgc gaaagaaggg ccggcccatg
     2161 tagaaaaaag atctttgaaa tcactgcgac tcctcgggag cgtcggcgaa cccatcaatc
     2221 cggaagcgtg ggagtggtat ttcaagcacg tcggaaaaga aaaatgtccg atcgtggata
     2281 cctactggca aactgaaact ggatcgatca tcatttctcc tctccccgtc ttcgcactta
     2341 aacccggaag tgctacgctc ccgtttttcg gaattcgtcc agtgctcctg gacagcgatg
     2401 gaaagaagat agaaggagaa ggctcaggaa acctggcgat cgaatttccg tggccttcca
     2461 tcatgcgagg agtctacgga gatccagatc gttttcacca gacatacttc actcaattca
     2521 aaggatatta tttcacggga gatggagcgc gtcgcgacaa agacggctac tactgggtca
     2581 ctggccgcgt ggatgatgta ttgaatgtgt ccggccatcg cattggcagt gcggaagtgg
     2641 aacatgccat cgtagagcac cgcgccgttg cagaatcagc tgtggtgggt tatccacacg
     2701 acatcaaagg tcagggcatc tacgcgtatg tgactctcaa gaatggaatt gaagcatcag
     2761 aggaattgaa acacgagatc ctcaacactg tgtctgagaa gattggcaag attgcgcgcc
     2821 cggatatcat acactgggct ccgggacttc ctaaaaccag gtctggaaaa atcatgcgac
     2881 gtatcctgcg aaaagtagcg gagtttgaat tcggttctct gggcgatatt t
//
LOCUS       DAOQJL010000498         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23053_length_2929_cov_5.176061, whole genome shotgun sequence.
ACCESSION   DAOQJL010000498 DAOQJL010000000
VERSION     DAOQJL010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23053_length_2929_cov_5.176061"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLG78_16905"
     CDS             complement(<1..361)
                     /locus_tag="PLG78_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE24855.1"
                     /translation="MVSDQQIAEQAVKIRRLIVRMVHAAKCGHPGGPLGMADIFASLY
                     FDPEIFRHNPKRTDWPDRDRLILSNGHTCAVRYAAMCASGYFTEKEVMTFRKLGSPFQ
                     GHPSTKFLPAMENSSGSL"
     gene            470..1102
                     /locus_tag="PLG78_16910"
     CDS             470..1102
                     /locus_tag="PLG78_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24856.1"
                     /translation="MQSDLEKAQAEIIRVQIERFHHFYADYFKREETHAMVEYFFDRI
                     YGLESDREWKRLALTTFQTVKHLVKEETRANIEHLMELNELTEELDADLARLLIDRHG
                     AEHRLSVNEYRDLYCSSSSPEKRRRHLELVLANMESFYQLAHRPINAYIMKPARFMTK
                     MLGIYPLFKTVEEGYYAVLPVTKELFESFVEEVSRREWEYLNSAFANAQT"
     gene            1118..1642
                     /locus_tag="PLG78_16915"
     CDS             1118..1642
                     /locus_tag="PLG78_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE24857.1"
                     /translation="MNQHKPELKEFAALSSAALTESFNHGYAKIDARVLQLTDVQLDR
                     TFAKDSVAGEWSCRLLLGHLADAEIAFSHRMRRVAGEDKPALAAWDENEFITKGLYSA
                     LSASDSVDLIRSVRQWTGAWLRTLSQAQFDRTGQHPQRGEQSLHTILAYDTWHLEHHC
                     WFLDRKLEQIYSAT"
     gene            complement(1627..2196)
                     /locus_tag="PLG78_16920"
     CDS             complement(1627..2196)
                     /locus_tag="PLG78_16920"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNE24858.1"
                     /translation="MNYLAHIYLSGEDEDLMIGNFIADSVRGNQLADFPEGVRKGIVL
                     HRKIDSYTDRHPVVTESKNRLRPEFGKWPPVIVDVFYDHFLAAGWEKYHAEPLPAYSK
                     RVYKILKKRKELLPERVRGFLPYMIYGNWLVGYSKLSGVEQALRGMSRRSRFNPEMHK
                     AVDVLKKQYEGFQDEFERFFLDLQAHVAE"
     gene            complement(2206..>2929)
                     /locus_tag="PLG78_16925"
     CDS             complement(2206..>2929)
                     /locus_tag="PLG78_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE24859.1"
                     /translation="LKLHPTCTIFARFNSKIDMWHGPPQSVVIKDGHNQKGTGYGFWL
                     EVAPFRPTLTSAIMPFYGARQFRNIKDYTKFNAGIVLVRDGADGATKGKVEWSWGKRK
                     VHYEITRTDGTNMLLGLKMLAEVQAAAGATELIFPFTDMLEEFKVVPGSKFDWVLSRS
                     FEPGVLPIGSAHPHGSIQASSDPKTGAIDPNFELYGHRNIFVMDASWIPTGLSVNPQI
                     TTMSSALRAARKLAGEKSKRLD"
BASE COUNT          788 a          749 c          700 g          692 t
ORIGIN      
        1 caagagagcc agaggaattc tccatggccg gcagaaactt ggtagatgga tggccctgga
       61 aaggagagcc gagcttgcga aaagtcatga cttctttttc cgtgaaatag ccggacgcgc
      121 acatggccgc atagcgaacg gcacatgtgt gaccattgga aagaatcaaa cgatcgcgat
      181 ccggccagtc cgtgcgcttt ggattgtgac ggaagatttc tggatcaaaa tagagtgagg
      241 cgaaaatatc cgccattccc aggggcccac ctggatgccc gcatttggcc gcatgaacca
      301 tgcgcacaat caatctgcga attttgactg cctgctctgc aatctgctga tccgaaacca
      361 tgattagact gaaaatcctg ggccgttagc ctgtaaacga tttcgccaac ctcaagtatg
      421 ccctcgaaag aggcatgaca ggctcgcgct acctgccagt tttgcaggca tgcagtccga
      481 tctggagaag gcgcaggctg aaatcatccg agttcaaata gaaaggttcc accacttcta
      541 cgcagactac ttcaagcgcg aagagacgca tgcgatggtc gaatatttct tcgacaggat
      601 ctatggcctg gaaagcgacc gagaatggaa gcgtcttgca ctcactacat tccagacagt
      661 caagcacctg gtcaaagaag aaactcgtgc gaacatagag catctgatgg aactcaacga
      721 actcacagag gaactcgacg cagatctggc cagactgctg atcgatcgcc atggcgcaga
      781 acacagattg tccgtgaatg aatatagaga tttgtactgc tcttccagtt ctccagaaaa
      841 acggcgaagg cacctcgaac ttgtactggc aaacatggaa tcattttatc agctcgcaca
      901 tcggcccatc aatgcataca tcatgaagcc tgcaaggttc atgactaaga tgctgggcat
      961 ttatcctctg ttcaaaactg tggaggaagg atactatgca gttcttccgg tcacaaaaga
     1021 attgttcgaa tcattcgtgg aagaagtatc gcgacgcgaa tgggagtatc tgaacagcgc
     1081 attcgcaaat gcgcagacct gagaaaggaa tcgatagatg aatcagcaca aaccggaact
     1141 caaagaattt gcagccctgt ccagcgccgc acttacggaa tcatttaatc atgggtatgc
     1201 gaaaatagat gcgcgagtgc tccaactgac ggacgtgcag cttgatcgca cgttcgcaaa
     1261 agattcggtc gccggggaat ggtcatgtag actcttactc ggacacctgg cagatgctga
     1321 aattgcattc agccatagaa tgagacgggt ggccggagaa gacaaacccg cgctcgcagc
     1381 atgggacgaa aatgaattca tcacgaaggg tctatactct gccctttccg cttctgattc
     1441 tgtggatttg atccgaagcg tgcgacaatg gaccggagcc tggctgagga ctctttcaca
     1501 agcacagttc gacagaacgg gtcaacatcc acaaagagga gaacaatcgc tgcacacgat
     1561 ccttgcctat gatacatggc acctggaaca ccattgctgg tttctggatc gaaagttaga
     1621 acagatttac tcggcaacgt gagcctggag atccaggaag aatctttcga attcatcctg
     1681 aaacccctcg tattgctttt tcaagacatc cactgcttta tgcatctcag gattgaacct
     1741 ggatcttctg gacattcctc gaagcgcctg ctccactcct gagagtttgc tgtaccctac
     1801 caaccagttt ccgtaaatca tgtacggcaa gaatccacgc actcgttctg gaagcagttc
     1861 ctttcttttc tttagtattt tgtatacccg tttagagtac gcgggcagag gctcagcatg
     1921 atatttttcc cagcccgcgg ccagaaaatg atcataaaaa acatcaacaa tcacaggtgg
     1981 ccacttcccg aactcagggc gcaggcgatt cttgctctct gtcacgaccg gatgacgatc
     2041 cgtataagaa tcaatcttac ggtgaagaac aattcctttt ctaacgcctt ctggaaaatc
     2101 agcgagctga ttgcctcgca cagaatctgc aataaaattg ccgatcatca aatcctcgtc
     2161 ctctccggac aggtagatat gtgcaaggta attcattccg tctgatcaat ccagacgctt
     2221 cgacttttct ccagcaagct ttcgagctgc acgaagcgca gaactcatgg tcgtgatttg
     2281 cggattcaca gacaatccgg ttggaatcca ggaagcgtcc atcacgaaga tgtttctatg
     2341 tccatagagc tcaaaattcg gatcaatggc tcctgttttt gggtcactgg aagcctgaat
     2401 cgatccgtgc ggatgagcag aaccaatggg caaaactcca ggctcaaaac tccgcgaaag
     2461 aacccagtcg aactttgaac caggcaccac cttgaattct tctagcatgt ccgtgaacgg
     2521 aaagatcagt tctgttgcac ccgccgccgc ctggacttcc gcaagcatct tgaggcccag
     2581 aagcatgttg gttccatctg ttcgcgtgat ttcgtagtga acttttcttt ttccccagct
     2641 ccattccact ttgccctttg tggctccgtc tgctccatcg cgaaccagaa caatgcccgc
     2701 attgaacttc gtgtaatcct taatatttct aaactgtctg gctccataaa aaggcatgat
     2761 cgcagaagtt agagttggcc ggaagggcgc cacctccagc cagaaaccat agccagttcc
     2821 tttctggttg tgcccatcct tgatcactac agattgcggt ggaccgtgcc acatgtcgat
     2881 tttggaattg aagcgtgcga agatcgtgca ggttggatgg agtttcaaa
//
LOCUS       DAOQJL010000499         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23077_length_2928_cov_3.774452, whole genome shotgun sequence.
ACCESSION   DAOQJL010000499 DAOQJL010000000
VERSION     DAOQJL010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23077_length_2928_cov_3.774452"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="pilM"
                     /locus_tag="PLG78_16930"
     CDS             complement(<1..804)
                     /gene="pilM"
                     /locus_tag="PLG78_16930"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNE24860.1"
                     /translation="MSILARKELVVDYGSTHIKGALVESSPLGGRRILRLETLPIVSL
                     RTDGDNQAEEYEYNLVRFVQSFFPEEESFLLNLPMDRVYVRDIRVPVVTAKQIDEVMP
                     FEVEPLLPVSLENAEVIGQPWAIGEESSNVITFTAQHKDLQRTVLPILRGTASVQMLS
                     VDCVGLAGASTLIPAEVSQDKMVGQIDIGGSYTIVNFVKNGTLVFTRRIAVGGVHITD
                     VVAETLGLDISTAEDKKIELDIDLAEDTEKTERSESYYRRHRIDKKTLTK"
     gene            complement(805..1944)
                     /locus_tag="PLG78_16935"
     CDS             complement(805..1944)
                     /locus_tag="PLG78_16935"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNE24861.1"
                     /translation="MINWLLKIPGIKSRRGAMAILFTFALGAAANTIAAKFFSDAQIQ
                     FASVKVAADGFRARQLALAGFQAGLAALRAAPEEYLYTSGLALKPPDISVSADCKPGC
                     FVSYRLLPEDGRLNLNNLVHSFDDKPNEQYRAVFKRFFPQYDISQENVDSLIDWIDED
                     DSPTGNGGENSYYASLKPPRKCKNFRMFSLGEITLIKGFTSDMIFASRAPANWEKTQK
                     ELSFQTEDEKNLITMDDWVAANDLTAYVPLGDRMEDKINVNAARYHTLMALSESMSRE
                     AVLALFKYRRQKGGYIKELGDLRNLPEFQRKTPADITLFDELAGNAGKLAGLLKTEGE
                     IYRIVGVGTITSGSSSSDNAKGAVIRRVTALYDKNNNKVIYYSED"
     gene            complement(1946..2578)
                     /locus_tag="PLG78_16940"
     CDS             complement(1946..2578)
                     /locus_tag="PLG78_16940"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNE24862.1"
                     /translation="MKRTSRHGFTLIELSIVIMLLATLFTVMFSTFFSVSRIASTASP
                     RSRELADAMLALEILRASISQTYYHPDAERVVFAARPDPDTRSDRITFSAVHPVSGTG
                     GSIVREVSFYLKEMPVQPDQGEKRYILMKREEEPVDKKPGEGGLHYPLLENVTGLHFK
                     YSLNGREWMDKWNTKQSRRIPRLIQIQLTVRIGGKARTLETLASPGLYLN"
     gene            complement(2575..>2928)
                     /locus_tag="PLG78_16945"
     CDS             complement(2575..>2928)
                     /locus_tag="PLG78_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24863.1"
                     /translation="LMSNPALTPTGDKPMEGNFGQEGGLYAGYGYEITIKEERIDLAK
                     VAAAGELKGTSVTDQLPASTQNNVSGTVKQVTGSETGGLVDIVRIIIKIKYPKGQGTF
                     GEYRVETFKGAPKKV"
BASE COUNT          653 a          762 c          713 g          800 t
ORIGIN      
        1 cttcgtcagc gtctttttat ctatcctgtg tcttctgtaa taggactcgc ttctttctgt
       61 tttttctgtg tcttctgcca gatcaatatc cagttcaatc tttttgtctt ccgcagtgga
      121 aatatccaga cccagtgttt cagcgaccac atccgttatg tgaactccac ccaccgcaat
      181 tctgcgtgtg aagacaagag tcccattctt tacaaagttt acgattgtat agctgccgcc
      241 tatgtcgatc tgccccacca ttttgtcctg cgacacttcc gccgggataa gggtggaagc
      301 accggccagg cccacgcagt ctacagagag catttgcacc gaggctgtcc cgcgaaggat
      361 aggcagaacc gtgcgttgca agtccttgtg ttgcgcagtg aaagtaataa cgtttgaact
      421 ttcttcgcct atcgcccacg gttgcccgat cacttcggcg ttttcgagcg aaacggggag
      481 aagaggttct acctcaaaag gcatgacttc atctatttgt ttggctgtta ccactggaac
      541 gcgaatgtcg cgcacataga cgcggtccat gggcaagttc aacaggaaag attcttcttc
      601 tggaaaaaaa gactgaacga aacgaacgag gttgtattcg tactcttctg cctgattgtc
      661 tccgtccgtt ctcaaggaca caatgggcag agtctcaagg cgcagaattc tccgtccccc
      721 gagagggctc gattcgacca gggcaccctt gatatgggtg ctcccatagt ccacgacgag
      781 ttcttttcgc gccagaatcg acattcagtc ttccgagtag tagatcacct tgttgttgtt
      841 cttatcgtag agagcggtca cacggcgtat gactgcccct ttcgcattat cggaactcga
      901 tgagccggaa gtgattgtcc cgaccccaac aattcggtag atttcacctt cggttttcag
      961 caatcctgcc agttttccag cgtttccggc caattcatca aatagagtga tgtctgcggg
     1021 agtctttctc tggaattcgg gcaggtttcg caaatcgccc agttccttga tgtatccgcc
     1081 tttctgtcgt ctgtacttga agagagcaag gaccgcttcc cggctcatcg attcagaaag
     1141 agccatcaga gtgtgatacc tcgcggcatt cacattgatt ttatcttcca tacgatcgcc
     1201 cagggggaca taagcagtga ggtcattggc cgcgacccag tcatccattg tgatgaggtt
     1261 cttttcgtct tctgtctgga aggagagttc cttttgcgtc ttctcccagt tagccggtgc
     1321 gcgggatgcg aagatcatgt cagaagtgaa ccctttgatg agagtgattt ctcccagaga
     1381 gaacattcta aagtttttac actttcgcgg cggctttaga gaagcgtagt acgaattttc
     1441 gccgccattt cccgtaggag agtcgtcttc gtctatccag tcgatgagtg aatccacgtt
     1501 ctcctgcgag atatcgtact gaggaaaaaa tcttttgaat accgcgcggt actgttcgtt
     1561 tggcttgtcg tcaaagctgt gcacaaggtt gtttagattg agccttccgt cttcaggcag
     1621 gagcctgtag gaaacaaaac aacccggctt gcagtctgcc gagacagaaa tatccggtgg
     1681 cttaagagcc agtcctgatg tatatagata ttcctctgga gccgcgcgca aagcggcgag
     1741 gccggcctgg aatccagcca gagcaagctg ccttgcgcga aagccatctg ctgccacctt
     1801 cacagatgcg aattgaattt gagcgtccga gaaaaatttc gcagcgattg tgtttgcagc
     1861 cgcgcccagg gcaaacgtaa atagaatcgc cattgcacct ctgcgactct tgattccagg
     1921 gattttaagc agccaattga tcatattagt ttagatataa acccgggctg gccagagttt
     1981 cgagagttct tgcttttcca ccaatgcgaa ctgtgagctg gatttgaatc aacctcggaa
     2041 tgcgccggga ttgctttgta ttccacttat ccatccactc tcttccgttc aatgaatact
     2101 taaagtgcag tcccgttaca ttctcaagca gtggatagtg cagaccgcct tctcctggtt
     2161 tcttgtctac aggctcttct tctcttttca tgaggatgta tctcttttcc ccttgatccg
     2221 gctgtactgg catttccttg agatagaagg aaacctctct tacaatcgaa ccacccgtgc
     2281 cggaaaccgg atgaaccgca gagaacgtga tgcgatccga tctggtatcc ggatccggtc
     2341 tggcagcaaa aacaactctc tctgcgtctg gatgatagta agtctggcta atagaagcgc
     2401 gcagaatctc cagagcaagc atggcatccg cgagctcgcg gctccgcgga gaagcggtgc
     2461 ttgcaatgcg cgaaacgctg aagaatgtgc tgaacatcac tgtaaacaga gtggccagaa
     2521 gcatgatcac aatggaaagc tctatgaggg taaaaccatg cctggaggtg cgcttcatac
     2581 tttcttgggt gctcccttaa aagtctctac gcgatactca ccgaacgtgc cctgcccctt
     2641 tggatacttg atttttatta tgattcgcac gatgtccacc aggccgccag tttccgatcc
     2701 ggtgacctgc tttacagttc ctgaaacatt gttctgagtg gaggcaggga gctggtctgt
     2761 gacgctggtt cccttgagtt ctcctgcagc cgccaccttc gcaaggtcga ttctctcttc
     2821 ttttatagta atttcgtagc catagcctgc gtacagtccg ccctcctgcc cgaagtttcc
     2881 ttccattggc ttgtctcccg ttggagtgag tgcggggttg ctcatcag
//
LOCUS       DAOQJL010000500         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23091_length_2927_cov_4.539694, whole genome shotgun sequence.
ACCESSION   DAOQJL010000500 DAOQJL010000000
VERSION     DAOQJL010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23091_length_2927_cov_4.539694"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2927)
                     /gene="mfd"
                     /locus_tag="PLG78_16950"
     CDS             complement(<1..>2927)
                     /gene="mfd"
                     /locus_tag="PLG78_16950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNE24864.1"
                     /translation="RILPYEMVRVSRDVEGEREQGLSALAHGKPVFTTADALCERFYP
                     SRDQLTLKVNDEIGPDDLAASLVRLGYRPSGRTEVPGEFTRKAGIIDVFASNLPEPIR
                     IDYFDTIIESIRKFDAESQIGGEKLINVGIGSAAETILSESQSKDLREKLKKSFKSEI
                     LPPWIDEEFGVINSRDCESLHEFLPLILEGKTLSELAGKKPLLIMADCDSVRARLDLF
                     EREVRECFAAASSTHACVPCEKAYHFSPLDGFDVLEIEDSEFPGDCGIQRLEGVKGRI
                     ADIQKAVGKLVLEGQKVFITAPDRTQLDRIAGVFRSQTDYKTESVVDFQMDAKKRGLV
                     SLVVSPLREGIRIPSLSTQILTDADLFGRGYKKKSYFKKRKSSPIESFLDLKEDDHVV
                     HVNHGIGKFIGLERVSAAGRERDFLVIEYADKDRLFVPLDQISLIQRYSAPTDEPRLD
                     SLGKASFKKIRERVAERTEQLAQELVNIYAARMKTPGYPFPADTIYQDEFEGAFEWEE
                     TPDQLAAIESVKADMEAPRPMDRLICGDVGYGKTEVAIRAAFKAVMAGKQVVFICPTT
                     VLALQHFRTFSKRYAEYPIKVDWMSRFRTAKEVADVKRRATSGEVDVVIGTHSLLAGD
                     VRFKNMGLLVIDEEQRFGVAHKEALKKMKVLVDVLTLSATPIPRTLHMSLAGIRDLSI
                     IETPPRNRLPVMTHVMEDSDSQFKEAVTRELERGGQVFYLHNRIDTIERAAARVRSLI
                     ANVQVGILHGRMEDDEVEDILMDFVDNKYQVLVTTTIVENGIDIANANTLIVDDADHF
                     GLSQLYQLRGRVGRSDRQAYAYFFFREKRALSETAMKRLNTILEYQELGSGFKVAMRD
                     LEIRGAGNILGKEQSGDIMDVGFEMYIKLLEDAVARIRGEEHRIDVHTGINLNQDMYL
                     PESYIPDTRQRIEFYKRFEAADSEEEIDEVLALMQDRFGEAPAEVTTFVATERIRVLG
                     SLA"
BASE COUNT          687 a          811 c          656 g          773 t
ORIGIN      
        1 ctgccagact gccgaggaca cgaatgcgct cggtagcaac aaatgtagtc acttctgctg
       61 gcgcttcgcc aaatctgtct tgcatgagag ccagcacttc gtctatttcc tcttcgctgt
      121 ctgcagcttc gaatctcttg tagaattcaa ttctctgcct ggtatccggg atgtacgatt
      181 ccggaagata catatcctgg ttcagattga ttccagtgtg cacatcgatt ctgtgttctt
      241 ccccgcggat gcgcgccacc gcgtcctcga gaagcttgat gtacatttcg aagccaacat
      301 ccatgatatc gcccgattgc tcttttccca gaatgttccc tgctccacga atctccaggt
      361 ctctcatggc gactttgaat ccgcttccca gttcctgata ttctagaatc gtattgaggc
      421 gtttcatcgc agtttcagaa agcgcccgtt tctccctgaa aaagaaataa gcatacgcct
      481 gcctgtcgct tctgcctacc ctgccgcgga gctggtagag ctgcgaaagc ccaaagtgat
      541 ccgcatcatc cacgatcaga gtgttcgcgt ttgcgatatc aattccattt tctacgatgg
      601 tcgttgtgac cagaacctga tacttgttgt ccacgaaatc catcaggatg tcttctactt
      661 catcgtcttc catccttccg tgaaggatcc ccacctgaac attcgcaatc aaagagcgca
      721 cgcgcgctgc tgccctttca atcgtgtcga ttcgattgtg cagatagaaa acctggcctc
      781 cacgttcgag ctcacgcgtt acagcctcct tgaactgcga atccgaatct tccatcacat
      841 gagtcatcac tggaagacga ttcctcggag gagtttcaat gatggacaaa tcccgaatgc
      901 ctgcgagaga catgtggaga gtccgcggaa tcggagtggc agatagagtc agcacatcca
      961 cgagcacttt catcttcttg agcgcttctt tgtgggccac accaaagcgt tgttcttcgt
     1021 cgattaccag aagtcccata ttcttgaagc gaacgtcacc cgccagaagc gagtgagtgc
     1081 ctatgaccac gtccacttcc ccggacgtag cccttctctt cacatccgcc acttccttcg
     1141 cagtacgaaa acgactcatc cagtccactt tgatcgggta ttccgcataa cgtttggaaa
     1201 acgtgcgaaa gtgctgcaga gcaaggacag tcgtcgggca aatgaagacg acttgcttgc
     1261 ctgccatcac cgctttaaag gcggctcgga tggctacttc tgtttttccg tatcccacat
     1321 ctccacaaat caagcggtcc atgggacgcg gagcttccat gtccgccttc acagattcta
     1381 tggctgccag ctggtccggc gtttcttccc actcaaaggc accttcaaat tcgtcctgat
     1441 aaattgtatc tgccgggaag ggatatcccg gagttttcat gcgcgcagca tagatgttga
     1501 cgagttcctg agccagctgc tctgttcgct cggccaccct ctctctgatc tttttaaaag
     1561 atgctttgcc aagagaatcc agccttggct cgtcggtggg tgcagaatac ctttgaatca
     1621 aggatatttg atccagaggc acgaacagtc tgtctttgtc tgcgtactct atgaccagaa
     1681 agtcgcgctc tctgcccgct gcactgacgc gctcaaggcc aatgaatttt ccaatgccgt
     1741 gatttacgtg aactacatga tcgtcctctt ttagatccag aaaggattca atgggagacg
     1801 attttctttt tttgaaatag gatttcttct tgtaccctcg gccaaacaga tccgcgtccg
     1861 taagtatctg agtggaaagg gagggaatgc gaattccttc gcgcaaggga gaaactacaa
     1921 gagataccag acctctcttc ttcgcatcca tctgaaaatc aacaacgctt tccgttttat
     1981 agtctgtttg agatcgaaaa acacccgcta tgcgatctaa ctgtgtgcga tccggtgcag
     2041 tgatgaagac tttctggcct tccaggacca gtttcccaac tgctttttga atatctgcga
     2101 ttctgccttt gactccctcg agacgctgta tgccacaatc gcccggaaac tctgaatcct
     2161 caatctcaag aacatcaaag ccgtctaacg gtgaaaagtg atatgctttc tcacagggaa
     2221 cgcaggcatg tgtggagctt gccgccgcaa agcattcgcg cacttctcgc tcaaacaaat
     2281 ccagacgcgc gcggactgaa tcacaatctg ccataatcaa gagtggcttt ttgcccgcga
     2341 gctctgagag agttttgcct tcgagtatca ggggaagaaa ttcatgaagt gattcgcaat
     2401 cgcgagagtt gatcacacca aactcttcat cgatccaggg gggtagaatt tccgatttaa
     2461 aggacttctt tagtttttcg cgcagatcct tcgattgact ttcagacaga atcgtttctg
     2521 ccgcagatcc aatccccaca ttgatgagtt tttccccgcc aatctgactc tctgcatcaa
     2581 acttgcgaat gctttcgatg attgtatcaa agtaatcaat acgaatcggc tctggcagat
     2641 tggacgcaaa cacatcgatg atgcctgcct ttcgcgtaaa ttctcctgga acctctgttc
     2701 tgccagaagg cctgtacccc agacgaacca gagatgctgc caggtcatcc gggccaatct
     2761 catcgttcac tttcagagtg agctggtctc tggatggata aaatcgttcg cataacgcgt
     2821 ccgcagtcgt gaacacaggt tttccatgag ccagagcgga aagcccctgc tctctctctc
     2881 cctccacatc tcgtgatact cgcaccatct cataggggag aatccgc
//
LOCUS       DAOQJL010000501         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23154_length_2921_cov_4.864271, whole genome shotgun sequence.
ACCESSION   DAOQJL010000501 DAOQJL010000000
VERSION     DAOQJL010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23154_length_2921_cov_4.864271"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLG78_16955"
     CDS             <1..356
                     /locus_tag="PLG78_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNE24865.1"
                     /translation="SLGADAVYIGTRLIATKEAAAGEDYKKQLVEAFPEDIVYTEKIS
                     GIPANWLKQSVEKAQASGETETKSLDEYKRWRDIWSAGQGVAQIHGVLSAKEVVDAML
                     KEYEQAVSALPRPVY"
     gene            554..2242
                     /gene="kdpA"
                     /locus_tag="PLG78_16960"
     CDS             554..2242
                     /gene="kdpA"
                     /locus_tag="PLG78_16960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNE24866.1"
                     /translation="MKGNDFVEIVIYFLLLTAGGLAGGLYMARVLAPDAQSVLATVES
                     NILRLLRIQKKGMDAREYLIAVLWFSLFSFLSVFLLMLAQNQLPLFPEKARLTASSDP
                     VSVGLAFNTAMSFMTNTNWQAYSGESTLSYFVQMTGLTVQNFASAAVGMAVMLVMFRG
                     FTRSETKDIGNFYADLFRITFYVLLPASILVAVFLSSQGVVQSFGAYAKAHTLEGAEQ
                     IVPLGPAASQIAIKQIGTNGGGFFGVNSSHPLENPTPLSNFVEVYSILWLAAACVFLY
                     GKMTGSMGRAWTIYIAMSVLLVSGLIIAAYAESAYSFGSTAMEGKEVRFGIGDSVLWA
                     IATTAASNGSVNAMHDSMMPLTGAVELFNMHLGEIVFGGVGSGMYGMLFYVILTVFLA
                     GLMVGRTPEFSNKKIEKFEVQMAGLAILGPGVAILFFTATALLVKAGTATLNNSGPHG
                     FSEVLYAFTSASANNGSAFAGLGVNNDFYNYALGACMLIGRFVCLIPAILIGGSLALK
                     KSVPESTGSFRTDTPVFVILLVFFILLIGGLTFLPALTMGPIVEHFVLKAGSLF"
     gene            2322..2921
                     /locus_tag="PLG78_16965"
                     /pseudo
     CDS             2322..2921
                     /locus_tag="PLG78_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
BASE COUNT          691 a          724 c          755 g          751 t
ORIGIN      
        1 tgtctctggg cgcggatgct gtctatattg gaacgcgatt gatcgccacg aaggaagcag
       61 ctgcgggaga ggattataaa aagcaacttg tagaggcctt tcccgaggac atcgtgtaca
      121 cagaaaaaat ctcaggaatc cccgccaact ggctcaaaca gagtgtcgaa aaagcgcaag
      181 catcaggaga aacagaaacc aagagtctgg atgaatacaa gcgctggaga gacatctggt
      241 ctgcaggcca gggtgtagca cagattcatg gtgtattgtc cgcgaaagaa gtcgtagatg
      301 caatgctcaa agaatacgag caggcagtga gcgcgcttcc gcggccggtg tactgacctt
      361 tacactttct taacagtaaa caatcccgtc ctaatatgga tttgatgcat cgcctttgaa
      421 aggcgttgcc aggcacgcga gcgtcacttt gctcatggcg aggtgcaaaa tgattttcgc
      481 aatctccgcc atcgtgggcg gtctaatact catctacctg gtgtttacca tcgcgctgcc
      541 ggagaagttc taaatgaaag gcaacgattt tgtagaaatc gtcatctatt ttctattgct
      601 caccgccggt ggacttgcgg gcggattgta tatggcccgc gtgcttgcgc ctgacgcaca
      661 gagcgttctg gccactgtag aatcaaacat cctgcgtctt ctgcgaatcc agaagaaagg
      721 aatggatgcc agagagtatc tcatcgcagt gctctggttc agcctgtttt cgttcttgtc
      781 agtttttcta ctgatgctcg cgcaaaatca actgcctctt tttccagaga aggcaagact
      841 gactgcttct tctgaccctg tgagtgttgg cctggcattc aatacggcca tgagctttat
      901 gaccaacaca aactggcagg cctactccgg ggaatcgacg ctgtcttatt ttgttcagat
      961 gacgggcctc acggtacaaa acttcgcaag cgctgctgtt ggcatggctg tgatgctcgt
     1021 gatgtttcgc ggtttcacga gaagcgaaac aaaagatatt ggtaattttt atgcggatct
     1081 atttcgcata acgttctatg ttttacttcc tgcatccatt ctagtggcag tctttctgtc
     1141 ttcgcagggt gtagtccaga gctttggcgc ctacgcgaaa gcgcacacgc tcgaaggtgc
     1201 agaacaaatc gtcccgctcg gaccggctgc ttctcaaatt gcaatcaagc agatagggac
     1261 aaatggtggc ggtttcttcg gagtcaatag ctctcacccg ctggaaaacc ctacacctct
     1321 ctccaatttc gtagaggttt actctatcct gtggctcgcg gccgcttgcg tctttctcta
     1381 tggcaagatg accggatcca tgggtcgcgc ctggaccatc tacatcgcca tgagcgttct
     1441 actcgtgagt ggtttgatca tcgcggcata tgcagaatct gcatactcgt ttggatccac
     1501 cgcgatggaa ggaaaagaag tgagattcgg aattggtgat tcagtgctct gggccatcgc
     1561 gaccacagct gcgtccaacg gctccgtaaa cgcgatgcac gacagcatga tgcctcttac
     1621 aggtgctgtg gaattgttta acatgcatct aggagaaatc gtgttcggcg gtgtcgggtc
     1681 cggaatgtac ggaatgctat tctacgtaat acttacggta ttcctcgctg gtctgatggt
     1741 tggccgcacg ccggagttct caaacaagaa gatcgagaaa ttcgaagtgc agatggctgg
     1801 tctggcaatt cttggaccgg gcgttgccat cttatttttt actgcaacag cactgcttgt
     1861 caaagcagga acagcgacat tgaacaactc cgggccacat ggcttttcag aagtgctcta
     1921 cgcatttact tccgcatccg caaacaacgg cagcgcattc gcaggactgg gagtaaacaa
     1981 tgatttctat aactatgccc ttggcgcgtg catgctcatt ggccgctttg tttgtctgat
     2041 tcccgccatt ctcattggtg gttctcttgc gcttaagaaa tccgttccgg aatccactgg
     2101 atcgtttcgc acagacactc ctgtattcgt gattctgtta gtgttcttta tcctgctcat
     2161 cggtggtctc acctttttgc ccgcgcttac tatgggacca attgtagagc acttcgtcct
     2221 gaaagcaggc tcactttttt agctggagca atgatatgaa aaaattcagc gtattctcac
     2281 tcgacaagaa tatagtcggc gcggcattca tcgcagcgct tcgcaagcta gatccgcgcg
     2341 ctcaaatacg aaatccagtc atgttcatgg tgtatgttgg cgccatcatg acaagcatcc
     2401 ctgccttctg gcaaggaagc gctgcttctc tcagctttaa gattcagctg gtttcctggc
     2461 tgtggctgac catattattt gcgaatttcg caggggctct ggcggaagga cagacgaaag
     2521 ctagaacgga cgctcttcgc acaacccgtg gaagcgtgct cgcaaaaaag acctcgtcca
     2581 ttgaggagaa gaagttctct gtcgtgaacg gacagctcct tcgcaagggc gatctgtttg
     2641 tttgcgaagc cggagatgac attgccgctg acggagaaat catcgcaggc gcggccacag
     2701 tggatgaatc tgcgatcaca ggagaatccg cccctgtcat tcgagaaagc ggaggagaca
     2761 gatctgcggt aaccgcgggg acgcgtgtgc tcagtgatcg cattgtgatt cgtgtgactc
     2821 aaaatccagg tgattcgttt ctcgatcgca tgatcacgtt gatagaaggc gcaaaacgtt
     2881 ccaaaactcc ggcggaaatc gcacttacga ttgtactgtc c
//
LOCUS       DAOQJL010000502         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23171_length_2920_cov_5.265620, whole genome shotgun sequence.
ACCESSION   DAOQJL010000502 DAOQJL010000000
VERSION     DAOQJL010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23171_length_2920_cov_5.265620"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PLG78_16970"
     CDS             <1..1401
                     /locus_tag="PLG78_16970"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNE24867.1"
                     /translation="MSAKGAVGMDIDLDLVPLREKGMNAYEIMLSESQERMLVVGKRG
                     QENTIIRIFNKWGLEAVEIGRVTTDGRLKIRWQGKLCTDIPADSLVLGGGAPRYKRDT
                     SRPAYLDQVADANQLLQKRPPQKEGMLALLSSPNVCSRRPVIEQYDSEVGLARVLGPG
                     GDGGIARVPGTNKGISVSTDCNSRYVFLDPYKGAAQAVFESARNVAVTGAEPLGITNC
                     LNFGNPNIPENYYVFSECIRGMGDACRSMGIPVTGGNVSFYNESEDGPVFPTPTIGMV
                     GLVEDIGRAVFPWFSSPGDLIFVAGHFAPSFGGSEYLKLNTGKSAGQTADLNFDHEKQ
                     LIQFLLQGAKRRLFASARDLSIGGLSAALARSVLSRNFDPRATSSPRGFALSIAEPYK
                     SDLDLTLFGETNGCAIVTVKPGSEAQTEALAKASGIPLWKIGTVSSDAMFNYGGFQIS
                     YEEAAAAYEGGLAGVF"
     gene            complement(1404..1766)
                     /locus_tag="PLG78_16975"
     CDS             complement(1404..1766)
                     /locus_tag="PLG78_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24868.1"
                     /translation="MTILITAISLVDCRTVETGTEARVVDLRIAGNTDGVTIHRMDPG
                     GEKAVLRTDIRYYKLDLPAMQGGYSEALFLIRYNIHDARTYPIVRILRDGTSIQTLSI
                     QDLQNLPEDQGMRVLTLP"
     gene            complement(1790..2794)
                     /locus_tag="PLG78_16980"
     CDS             complement(1790..2794)
                     /locus_tag="PLG78_16980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE24869.1"
                     /translation="MSQEDLEKLKKTIEAYDKLSELSHKELLEAHQELQARESQQELS
                     REELIRLHEQVRGLENEKASLETRIRSVLHEDKANEARILSELDALRQESDAGFFVNL
                     FRVLCHHEFEPDAAMTHWADILKHSAEMQAKLGRPIGFRVAMMDYFINLNRVLKSPVI
                     LEIALYDEMMQNALMDELTGVYNRRYFERSLNREINRARRHSHSLSLLALDLDNFKEF
                     NDRHGHAAGDQVLAAFSDQLRKSFRAEDIVCRYGGEEFVVILPLTTPVNAMIVAQRFL
                     DEVRKLEIYGRSVTCSGGIAGYPEHESEPRGLFKAADRALFIAKKRGKDRILFADEVI
                     "
BASE COUNT          689 a          740 c          751 g          740 t
ORIGIN      
        1 atgagtgcga aaggtgctgt tggaatggac atcgatctgg atcttgtccc tctccgcgaa
       61 aagggaatga acgcgtacga aatcatgctg tcagaatccc aggaacgcat gctcgttgtg
      121 ggcaaacgcg gccaggaaaa cacgattata cgtatcttca acaaatgggg tcttgaggcg
      181 gtagaaatcg ggcgcgtcac tacagacggc aggcttaaga ttcgctggca gggaaaattg
      241 tgcacagaca ttccggcgga cagtctggtt ctgggtggcg gtgcacctcg ctacaaaaga
      301 gacacaagca gacctgccta cctggatcag gtggcagatg caaatcagct cttacagaaa
      361 agacctccgc aaaaagaagg aatgcttgcc cttctatcgt cgcctaacgt ctgctcacgc
      421 agaccagtca tcgagcagta tgacagcgaa gtgggtctgg ccagagtcct cggtccaggc
      481 ggggacggtg gaatcgcgcg cgttcctggg acaaacaaag gaatttcagt atccacggac
      541 tgcaacagca ggtatgtttt cctggatccg tacaaaggag cagcgcaggc tgttttcgaa
      601 tcggccagaa acgttgccgt taccggagcg gagcctcttg gaatcacaaa ctgcctgaac
      661 tttggaaacc cgaatatccc ggagaattac tacgttttca gcgaatgcat tcgcggcatg
      721 ggggatgcgt gcaggtccat ggggattcct gtaacaggag gaaacgtttc cttctataat
      781 gaaagcgaag atgggccagt atttccaacc ccaaccattg ggatggttgg tctggtagaa
      841 gacattggac gcgcggtgtt cccgtggttt tcctctcccg gagatttgat tttcgtcgct
      901 ggtcattttg ctccgtcctt tggcggaagc gaatacttga aattgaatac ggggaaatcc
      961 gccgggcaaa cagcggacct caatttcgat cacgaaaaac aactcattca atttctgctg
     1021 cagggcgcaa agaggagact ctttgcatct gcgagagacc tttctatcgg tggactttct
     1081 gccgctctcg caagatccgt tctttcgcgc aattttgatc cacgcgccac ttcttctccc
     1141 cgtggctttg cactgagcat cgccgagcct tacaaatcgg acctggattt aacgttattc
     1201 ggcgaaacaa acgggtgcgc gatcgtaact gtgaagcctg gaagtgaagc acagacagag
     1261 gccctcgcaa aagcatccgg aattccgctg tggaaaatcg gcactgtgtc ttcagatgca
     1321 atgtttaact acggtggctt tcagatcagt tacgaagaag cggccgcggc ctacgaaggc
     1381 ggactggctg gagttttttg agatcagggc aaggtgagta cgcgcattcc ctgatcctcc
     1441 ggtagattct gcagatcctg aatggaaagt gtctggatgg atgttccatc tcgcaggatt
     1501 cgaacgatcg ggtaggttcg cgcgtcgtga atattataac gaatcaggaa cagtgcttct
     1561 gaataacctc cttgcatggc aggcaggtcc agtttgtagt aacgtatgtc cgttctgagg
     1621 actgcttttt caccacccgg atccattctg tgaatggtca ccccgtccgt gtttcccgca
     1681 atcctcagat ccacgactct ggcttccgtg ccagtctcca cggttctgca atcgacgagc
     1741 gagatcgcgg taattagaat ggtcaacgtt aggcggcttt gaggcaacct cagattacct
     1801 cgtccgcgaa cagaattctg tccttgcctc tcttttttgc aatgaacaga gcccgatccg
     1861 ctgccttgaa caggccgcgt ggttcacttt catgttcggg ataacctgca attccaccgc
     1921 tgcaggttac acttctacca tagatttcaa gctttcgcac ttcgtcgagg aaccgctgcg
     1981 ctacgatcat cgcatttact ggagtagtta atggaaggat cacaacaaat tcttctcctc
     2041 cgtatctgca aacgatgtct tctgcgcgga aactctttct gagctgatcc gagaatgcag
     2101 ccagaacctg atccccggcc gcgtggccat gcctgtcatt gaattctttg aaattgtcaa
     2161 gatccagagc gagcaaagaa aggctgtggg aatgacgacg tgcgcggttg atttcgcgat
     2221 tcagcgaacg ctcgaagtag cgccgattgt agaccccggt caattcatcc atgagtgcgt
     2281 tctgcatcat ttcgtcatag agtgcgattt caagaatcac cggacttttc aaaacgcgat
     2341 tgagattgat gaagtagtcc atcatggcca cacgaaagcc aatgggccga ccaagcttag
     2401 cctgcatttc cgcagagtgt ttcaaaatgt ctgcccagtg tgtcatggcc gcatctggtt
     2461 caaattcgtg atggcacaac actctaaaca gattcacgaa gaatcccgcg tccgactcct
     2521 gtctgagtgc gtcgagttca gaaagaatgc gagcctcatt tgccttgtct tcatgcaaga
     2581 cactgcgaat gcgagtctcc agactggcct tttcattttc aaggcctcgc acctgttcgt
     2641 gcaggcgaat gagttcttcg cgggataact cctgctgaga ttcgcgcgcc tggagttcct
     2701 gatgggcctc cagtagctct ttgtggctca gttcggacaa tttgtcgtaa gcttctatgg
     2761 tcttttttag cttttctaag tcttcctgac tcatgtgcag actctttttt gctacctgaa
     2821 attgaccatc cactttttgt taggcgaatg aacgaactgg ctctctatgt tatgtccgta
     2881 ttctatatct ttgcgggcat catgcacttc gcgaaaccta
//
LOCUS       DAOQJL010000503         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23309_length_2910_cov_3.724694, whole genome shotgun sequence.
ACCESSION   DAOQJL010000503 DAOQJL010000000
VERSION     DAOQJL010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23309_length_2910_cov_3.724694"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PLG78_16985"
     CDS             <1..1347
                     /locus_tag="PLG78_16985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC12162 family protein"
                     /protein_id="HNE24870.1"
                     /translation="SFVWATNDDVWNSVLYGRVRPFVQGAPPARKIQASLGESYRLPG
                     HFGPPPRMSLARRLRRVPGLIRQRLVVRGTDSFIFNTYMQAEEEVLLQRGLGQTPRRW
                     HSHHFFGPSVDPDLRRELSASFSGDSSGLMGCMRALLFELIPVCYLEGYRDLVKKSES
                     MPWPDRPRLIFTSGNYDTDEIFKCWTASRVERGSRYMVGQHGNNYGTHRYIAYRTTDQ
                     LTSDRFLTWGWRDSLPSHTPAFILRTSGFPELDPAGGLLLAEDGLPNRVYTWDIFPDY
                     TSLINEQFEFVRALPPAIKERLLVRLHGPDENLDRFGDELRWNDFDRTVNLERGKVLF
                     ADVVRKSRIVVFGYDSTGMLESLASNSPMIAFWSGGLSHLKDHVKPQYEWLIDAGIVH
                     LSGESAARKISEIWDNVNAWWQSESVQSARVRFCEIYARRSRTPAADLLKIIKDDL"
     gene            1344..2546
                     /locus_tag="PLG78_16990"
     CDS             1344..2546
                     /locus_tag="PLG78_16990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE24871.1"
                     /translation="MTTAFFLDNQYRGGVDTFLIQLINHWPEDGRLRLYCNRHHPGLE
                     TIRAMVHRPLQISTYGDSISWTLFSGKARIPLDRFYVVRAAVVVFQRIMPPILFLANL
                     IRFSVRFRMSGAHSLLVVNGGYPASATARSASLAWRIAGKGGVNIHNFHSHASPSNGK
                     AKRMNDFLDRLILRASTYFVTVSRSCELSLRKRDVFLNSDRIRVIPNGIADPGFPLHR
                     PETQKLSCLMLGTYHEYKGHSFLLEAFAQIRERHATTELNMYGDGSEADRVRIEGLID
                     RFGLRECAFLHGFVTDPDALIRNADVLVLPSQAHESFGLTIVEAMARGVPVVATRVGG
                     IPEVLEDGVQGFVCSPSDASAFAAAISRLLSSEELRRDLGAAGRRRYEQHFRAEQMSK
                     AYHSLLCS"
     gene            2604..>2910
                     /locus_tag="PLG78_16995"
     CDS             2604..>2910
                     /locus_tag="PLG78_16995"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE24872.1"
                     /translation="MYILGIHDGHNSAACLLKDGKLVFAIQEERLTGIKNYWGHPLKA
                     IDACLKHEGITWAQVDHVAFAAFDGSSPGRPFISRDEYLEYTKHVLKPAPRAGLRARL
                     "
BASE COUNT          625 a          745 c          786 g          754 t
ORIGIN      
        1 tcttttgtgt gggccacaaa cgacgacgta tggaacagcg ttctctacgg ccgtgtgcgt
       61 cctttcgtcc agggtgcacc gcctgcgcga aagatccagg cgtccctggg cgaaagctat
      121 cgccttcctg gacattttgg gcctccgcct cgcatgtctc tggcgcgcag actgcgaaga
      181 gtacctggtt tgatccgcca gcgcctggtt gtgcgcggga cggattcctt tatcttcaac
      241 acatacatgc aggcggagga agaagtcctt cttcaaagag ggctcgggca aactccacgg
      301 cgctggcatt cgcatcattt ttttggccct tctgtggatc ctgatttgcg cagggaattg
      361 tccgcgagtt tctccggaga ctcttccggt ttgatgggat gcatgcgagc gcttcttttc
      421 gaactcattc ctgtttgcta cctggaagga taccgcgacc tggtgaagaa atcggaatcc
      481 atgccctggc cagatcggcc caggcttatt ttcacgagtg gcaactatga cacggacgaa
      541 atcttcaaat gctggacggc ttcacgcgta gaacgtggct cgcgctacat ggttggtcaa
      601 catggaaaca actatggaac gcaccgttat atagcatatc gcactacaga ccagctaacg
      661 tcggatcgat tcctgacatg gggctggagg gacagccttc cttcgcatac acctgcattc
      721 attttgcgca cttccggttt tcctgagctg gatccagcag gaggtctcct tctggccgag
      781 gatggtctac ccaatcgcgt ctatacctgg gacatctttc ctgactacac gagcctgatc
      841 aacgaacagt ttgaattcgt gcgcgcactg ccccccgcga ttaaggaaag actgctggta
      901 cgcctgcacg gtccagatga aaacctcgat cgatttggag atgaactcag gtggaatgat
      961 tttgatagga cagtgaacct ggagcgaggc aaagtcctct tcgcagacgt tgtgagaaag
     1021 agtcgcatcg tagtgtttgg ttacgattcc acgggtatgc tggagagtct tgcttccaat
     1081 agtcccatga ttgcattctg gtcaggcggc ctttcgcatt taaaggacca cgtaaagcct
     1141 cagtatgaat ggttgatcga tgcgggaatt gtccatctgt ccggtgagtc tgctgcgcga
     1201 aagataagcg aaatatggga caatgtaaac gcctggtggc aaagcgaatc ggtgcagagc
     1261 gcgagagtcc gcttctgtga aatctacgcg cgaagatccc ggactcctgc ggcggacctt
     1321 ctaaaaatca tcaaggacga tctgtgacca cggctttttt cctggacaat cagtaccgtg
     1381 gaggagtaga cacattcctg attcaattga tcaatcactg gcccgaggac ggtcgacttc
     1441 gcctctattg caaccggcat caccccgggc tagagacaat tcgagccatg gttcatcgtc
     1501 cgctgcaaat atccacgtat ggagattcga tttcgtggac actgttttcg gggaaggcta
     1561 gaattcctct ggatcgcttt tatgtagttc gcgcggccgt tgtggttttt cagagaatca
     1621 tgccgccgat tctctttctt gccaacctga ttcgtttctc cgtgcgtttt cggatgagcg
     1681 gtgcgcattc ccttcttgta gtgaacggcg gctatcccgc aagcgcgacg gcacgaagcg
     1741 cgtcccttgc ctggaggatc gcaggaaagg gcggcgttaa catacataat tttcacagcc
     1801 acgcttcgcc ttcgaacgga aaggcgaaga gaatgaacga ttttcttgac agattgattt
     1861 tgcgtgcgtc tacgtacttt gtaacggttt ctcgcagttg tgaactttcg ctgcgaaagc
     1921 gagacgtttt tctaaactcg gatcggattc gcgtgatccc caatggaatt gccgatccag
     1981 gatttcctct tcacagacca gagactcaga aattgagttg cttgatgctc ggaacctatc
     2041 acgagtacaa aggccattcg tttcttctcg aggctttcgc gcagatcagg gaaaggcacg
     2101 ccacaacgga actcaatatg tatggagatg gatcggaagc ggatcgcgtc cgcattgaag
     2161 ggttgattga tcgttttgga ctgcgcgaat gcgcgttcct gcatggcttt gtaactgacc
     2221 ccgatgctct cattcgcaac gcagatgtac tggttctgcc atcccaggcg cacgaatcat
     2281 ttggactgac gatcgttgaa gccatggctc gtggtgttcc tgttgtggct acgcgcgtcg
     2341 ggggcattcc agaggtgctc gaagatggcg ttcagggttt tgtttgttcg ccgtctgatg
     2401 cgtctgcatt tgcggccgca atttcgcgtc tgctttcctc ggaggaattg cgtcgcgatc
     2461 tcggggcggc gggtcgtcgg cgctatgaac agcattttcg cgctgaacag atgagcaaag
     2521 catatcacag tctcttgtgt tcttagctct aaattcaatt tgactcgggg ccgcaccggc
     2581 ttccacttgt agttctcttc aatatgtata ttcttggcat tcacgacggt cacaatagcg
     2641 ctgcctgtct tctcaaagac ggcaaactgg tctttgctat tcaggaagag cgcctaacgg
     2701 gcattaagaa ctactgggga catccgctca aggcaatcga cgcatgcctg aagcatgaag
     2761 gtattacctg ggctcaggtg gatcatgttg catttgccgc ttttgatgga tcttcgcctg
     2821 gtcgtccatt tatctccagg gatgaatatc tcgagtacac aaaacatgtt ttgaagcccg
     2881 ctccccgcgc cgggttacga gcgaggcttc
//
LOCUS       DAOQJL010000504         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23371_length_2905_cov_4.371228, whole genome shotgun sequence.
ACCESSION   DAOQJL010000504 DAOQJL010000000
VERSION     DAOQJL010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23371_length_2905_cov_4.371228"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLG78_17000"
     CDS             complement(<1..254)
                     /locus_tag="PLG78_17000"
                     /inference="COORDINATES: protein motif:HMM:NF020461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAD-like domain-containing protein"
                     /protein_id="HNE24873.1"
                     /translation="MAHFPGFQKKFPKPDSCQPMSKSLEEYQGRLPGSLIEFLSEQGA
                     CSYAKGLIWTMDPFFMQDTLEEWLDEPENAVPFARTAFGE"
     gene            357..617
                     /locus_tag="PLG78_17005"
     CDS             357..617
                     /locus_tag="PLG78_17005"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE24874.1"
                     /translation="MSLHPYARKREGENMIYQKNVPGWERAMRVGLGLLLVVAAAESI
                     YSGRPMLLSVGLLFSAIGIVATGFLGWCPACAMVGRKLKFKE"
     gene            614..1150
                     /locus_tag="PLG78_17010"
     CDS             614..1150
                     /locus_tag="PLG78_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE24875.1"
                     /translation="MNTQYDKNIVEAARSGDMQALERLLLQCQPDIARFARNVCATPE
                     DVEDAVQETLWIVSRKIGTLRTVSAFASWAFRVVKHECYRLFRRVRRELSTDRVAEQS
                     YLDPGVDVPAMMRQETIQALANMPDLFRKVIVMRDIEGIPAVQVASELGLSIPAVKSR
                     LHRARSWMRLTLGHWMES"
     gene            complement(1184..1876)
                     /locus_tag="PLG78_17015"
     CDS             complement(1184..1876)
                     /locus_tag="PLG78_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE24876.1"
                     /translation="MRTAVVFVLLSLNAWTACKEKPSFPSELTQYSEPVQGILPYFQG
                     TRMDPFWPHSKDRPADLRRMNNAIFRTEQDKPASALDHAKGKYSLVTFFYSSCVGLCP
                     LIAENMKNLSSRIEDQSDIQFISITIDPEIDDVKALQKFRAKHSIAQTNWILLTGDKK
                     ATESLAREQFAGEIKIKEGKAGLVDFVHTENVFLLDKEGYLRGVYRARGVGDFPRLLA
                     DLKRLRDGDRKN"
     gene            complement(1873..2466)
                     /locus_tag="PLG78_17020"
     CDS             complement(1873..2466)
                     /locus_tag="PLG78_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24877.1"
                     /translation="MIRSRFVFLFAAVLLLQDCSKQGPVIDAGSPGFEPKGIQLFYQG
                     KPFTGILRSKIPALDQVELAEYHAGLQHGITREQTLDGKLIAERPYYAGAKHGIHKTW
                     HENGQMKTFVEFKYGKYAGEAWSWYPSGKPFDYRKYNDQGELLLSRKWREDGQIYMNQ
                     VFQNGEAIGMPGSKLCDPAKNEKDQPASSNEKKENAP"
     gene            complement(2463..>2905)
                     /locus_tag="PLG78_17025"
     CDS             complement(2463..>2905)
                     /locus_tag="PLG78_17025"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNE24878.1"
                     /translation="DTLSTEASTFDQQQGHPEMTGSYHHHSQLINGDGTGGTPASVNL
                     TTQGSSALIGIALDGYAIYGRWCGDATSGQNTQATGLDACHGHITTTPEFSTATYHYH
                     YGGGASGTTCNGSGVGTGAGNDATAGIPTIMGSYFYGVIGQVSN"
BASE COUNT          652 a          683 c          760 g          810 t
ORIGIN      
        1 aattcgccaa atgcagtgcg tgcaaacgga actgcatttt ccggttcatc cagccattct
       61 tccagtgtgt cctgcatgaa gaatggatcc atggtccaga tcaatccttt cgcgtatgaa
      121 caagcgcctt gctcggataa gaattcgatc agcgatcccg ggagtcttcc ctgatactct
      181 tcaagggact tactcattgg ctggcaagaa tccggtttgg ggaatttctt ctggaaacca
      241 gggaagtggg ccatacccgg agaaaatggt ttgtaggttg cttgtcaaat cgctcccgac
      301 gaaccgctga atttttcgca tcggcaagaa aaagtctgaa tccttttgca atccaggtgt
      361 ctcttcatcc atatgcgcga aagcgcgaag gagaaaacat gatttatcaa aagaacgttc
      421 cgggctggga aagagctatg cgcgtaggcc tgggcttgct gcttgttgtt gcggctgcgg
      481 aaagtattta ctctggcagg ccaatgcttc tttcagtggg gctcctgttc tccgctattg
      541 gaattgtggc gacaggtttc ctgggctggt gtcctgcctg cgcgatggtt gggagaaaat
      601 tgaagttcaa ggaatgaata cgcaatacga caagaacatc gtagaggccg ctcgaagcgg
      661 agacatgcag gcactggagc gacttttgct acagtgtcag ccggacattg caaggtttgc
      721 tagaaatgtt tgcgccactc cagaagatgt ggaggacgca gtccaggaga ctctctggat
      781 tgtttcccgc aagattggaa cgcttcgcac agtctctgcg tttgcatcgt gggcgtttcg
      841 cgttgtaaaa catgaatgct atcgtctgtt tagacgagtc aggcgtgaac tttcaacaga
      901 cagagttgcc gagcagtcct atctggaccc gggcgtagat gttcctgcga tgatgaggca
      961 agagacgatt caggcgctgg caaacatgcc ggatctattt cgtaaggtga ttgtgatgcg
     1021 cgatattgaa ggaattcctg cggttcaggt tgcaagtgaa ctgggacttt ccatcccggc
     1081 tgtgaaaagc cgccttcatc gcgcgaggag ttggatgcgt cttaccctgg gacattggat
     1141 ggaatcttga tgttttcctt tttgacagaa aggatgatag agttcaattc ttacgatcac
     1201 cgtctcgcaa acgtttcaaa tcagcaagga gtcgaggaaa atcacccacg cctcgtgcac
     1261 gatacacgcc tcgcagatat ccttccttat ccaaaagaaa tacgttctca gtgtgcacaa
     1321 aatcaacgag gccagctttg ccttctttga tcttgatttc tcctgcgaat tgctctcgtg
     1381 ccagactttc cgttgctttc ttgtctcccg taagaagaat ccaattcgtc tgcgctatgc
     1441 tgtgttttgc gcgaaatttt tgaagagctt ttacgtcgtc tatttccgga tcaatggtga
     1501 tggagatgaa ctgaatgtca gactgatctt caattcgtga agatagattc ttcatgttct
     1561 ctgcgatcaa agggcagagt ccaacgcagg aagagtagaa gaaagtaacg agcgagtatt
     1621 tgcccttcgc atgatccagt gcgctcgccg gtttatcctg ttctgtgcga aagatcgcgt
     1681 tgttcattcg tctcagatcc gccggccgat ctttggagtg cggccagaaa ggatccattc
     1741 tcgttccttg aaagtaaggc agaattcctt ggacgggttc ggagtattgt gtgagctcag
     1801 aaggaaaaga cggtttttct ttgcaggcag tccacgcgtt caagctcaag agtacaaaaa
     1861 cgactgcggt tctcaaggtg cgttttcctt tttttcgttt gaggatgccg gctgatcttt
     1921 ttcattcttt gcaggatcgc acaatttgct gcctggcatt ccaatcgctt cgccgttttg
     1981 aaatacctga ttcatgtaga tctggccgtc ttcgcgccat ttcctggata gaagtagttc
     2041 tccctggtca ttgtattttc tgtaatcaaa aggtttgcca ctcggatacc aggaccatgc
     2101 ttctccagcg tacttcccat atttgaattc aacaaacgtt ttcatctgac cgttttcatg
     2161 ccaggttttg tgaattccgt gtttggctcc cgcgtagtaa gggcgttccg caatcaattt
     2221 gccgtctaac gtttgttcgc gcgtgatgcc gtgttgaagt cctgcgtggt actcggccag
     2281 ctctacctgg tccaatgctg gaatcttcga gcgaaggatt cctgtgaatg gcttaccttg
     2341 atagaagagt tgaatgccct ttggttcaaa acctggtgaa cctgcgtcta ttactggtcc
     2401 ttgtttgctg cagtcttgta agagaagaac tgccgcaaac aggaacacaa atctggatcg
     2461 aatcaattgc tgacttgacc aatcactccg tagaagtagg aacccatgat ggtcggaata
     2521 ccagccgtcg catcgttgcc tgcgcctgtt cctacaccgg agccgttgca cgtagttcca
     2581 gaagctcctc caccgtagtg atagtggtag gttgcagtag agaattccgg tgtagtagtg
     2641 atgtggccgt ggcatgcatc caggccagtt gcttgcgtgt tctgaccgga cgttgcatct
     2701 ccgcaccatc ttccgtagat tgcatatcca tccagggcga ttccgatgag tgcactggaa
     2761 ccttgcgttg ttaagttcac gctcgccggt gtgccgccgg tgccgtctcc attgattagt
     2821 tgcgaatggt gatggtagct cccggtcatt tctggatgac cttgctgctg gtcgaaagtg
     2881 gaggcctcag tcgaaagggt gtcgg
//
LOCUS       DAOQJL010000505         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23767_length_2876_cov_4.679546, whole genome shotgun sequence.
ACCESSION   DAOQJL010000505 DAOQJL010000000
VERSION     DAOQJL010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23767_length_2876_cov_4.679546"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLG78_17030"
     CDS             <1..1246
                     /locus_tag="PLG78_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24879.1"
                     /translation="AILPVIPRIVPPSGEWVREGYGKPGQETGVAQRNLPPGISLNER
                     GELVTDGWTDSDFDRAWEKFKHLPAHDRRSGVERRKNIDRRMSNDPKRKDRRSGEDRR
                     KQDLFKEREEFLRKLEEHKKRKQEYADWLANEELPVLPPVEMPVEEEKKKKEATIEIE
                     HATIHIGEFPKKEEEKDEEPEEEPEPEPEPEPEPEEEPEPEPEEEEEEEPKHIVFETP
                     EPDLQPVRMPAVGEFAGSHLEAARDLSSQPLLSLGMPSGMKVDDQPQLPAEAEAATAS
                     AGETAATEEETPEIPGLDEPEEAKEEKPQVQEIRGVLELKPPEEDDAPFLTLTYDFTK
                     IPDSFKLSRDYHTMEYAYYKYKPMLVKAQEFTRRKMLKNALNYYRVIKSQNIPPEFKR
                     MINRNIRDITEYLEKFLMSRAG"
     gene            1243..2604
                     /locus_tag="PLG78_17035"
     CDS             1243..2604
                     /locus_tag="PLG78_17035"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNE24880.1"
                     /translation="MTQATAFLAAILALAGGWLIYSQIRFLRRWFIPPALLGGLLLFC
                     AGPEVLSIVGKPERLFIRSWPPLLLGYLFAAIVLGFESGKSSRGLVGQVVQQNLFVWF
                     LSFGQLLGAYCIYLLFFRADVSPLIAHVVEIGWAGGPGSAAAMSAVMRRLGQSAIGDL
                     GLFSATLGQVWGALSGIWIVNHLNRKAQHVIGTEASSSHESARPLSSVEANGIADAEV
                     RRPRFPGPKGLLRIFLLFLLPLSAVFIAMAAKEGLGIVAIYTPRALFLGDIPLFSLAL
                     LCAFPVRWIAIRAGILTAENCETIQNINGQVLDWIILAALAALEPAAFIALLVPFSAM
                     MAFGAFFCFVLMYFFAPRMLPTNTWRELGLINYGMATGTTALGLMLLGLLRSHTKDEA
                     RLVYGMAVPFNAPFVGGGILSLTLPEWTARGGETIIPFLLLAWMFVFFVVCRRLQQKE
                     AHE"
     gene            2597..>2876
                     /locus_tag="PLG78_17040"
     CDS             2597..>2876
                     /locus_tag="PLG78_17040"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNE24881.1"
                     /translation="MSKMPVLFVGHGNPMLVLEDNEITQAWATLGQSLKPRAILAVSA
                     HWVTRGTLVTSMQKPRIIYDFYGFPDELYQVTYPAPGSPELALRTASLT"
BASE COUNT          731 a          749 c          754 g          642 t
ORIGIN      
        1 ggcaatcctt cctgtcattc cgcgcattgt acctccttca ggagaatggg tcagagaagg
       61 atacggaaaa ccaggacagg aaacaggagt agcacagaga aatcttcctc ctggaatttc
      121 actgaacgaa cgcggggaac tcgtaacaga cggctggacc gattctgatt ttgatcgcgc
      181 gtgggaaaag ttcaaacacc tgcccgcaca cgatcgacgc agtggagtag agcgcagaaa
      241 gaatatcgat cggcgaatgt cgaacgatcc aaaaagaaaa gacagacgtt ccggcgagga
      301 cagaagaaag caggatcttt ttaaggaacg cgaagaattc ctcagaaaac tggaagaaca
      361 taagaagcgg aaacaggaat acgcggactg gctggccaat gaggaacttc ctgttctccc
      421 gccggtggaa atgccggtag aagaagagaa gaagaagaaa gaagcaacca tcgagattga
      481 gcacgcgacc attcacattg gcgaatttcc gaagaaggaa gaggaaaaag acgaagagcc
      541 agaagaagaa cctgagcctg agccggaacc ggagcctgaa ccagaagagg agccagagcc
      601 agagcccgaa gaagaagagg aagaggaacc aaagcacatc gtctttgaga cgccggagcc
      661 ggatctccag ccggtgcgaa tgccagccgt gggcgaattt gcaggcagcc acctcgaagc
      721 agcgcgcgat ttatccagcc agccactctt gagtctggga atgccttctg gcatgaaggt
      781 ggacgatcag cctcagttgc ctgcggaagc agaagcagcc acagcaagcg caggcgaaac
      841 agcagcgaca gaagaagaaa ctcctgaaat tccggggctc gacgaaccgg aagaagcaaa
      901 agaggaaaag ccacaggtgc aggagattcg aggtgttctc gaactcaaac caccggaaga
      961 agacgatgcg ccattcctca ctctcacata tgatttcacc aaaatcccgg acagcttcaa
     1021 gctgagccgc gactatcata caatggaata tgcatactat aagtacaaac caatgctggt
     1081 caaagctcag gaatttacgc gaagaaagat gctgaagaat gctctgaact actatcgcgt
     1141 gatcaaatcg caaaacatac cgcctgagtt caaacgaatg atcaatcgca atatcagaga
     1201 tatcaccgag tacctggaaa agttcctgat gagccgcgca ggatgacgca ggccacggcg
     1261 tttctagcag ccattctggc acttgccggc gggtggctca tctattcgca aatccgtttt
     1321 ctcagaagat ggttcatccc gcccgcactc ctgggtgggc tcctcctttt ctgcgcagga
     1381 ccagaagtac tttcgattgt aggaaaacca gagagacttt tcattcgctc ctggcctccg
     1441 cttcttcttg gatacttatt cgctgccatc gttcttggat ttgaatccgg caaatcatcc
     1501 agaggactgg tgggtcaggt tgttcagcag aatctgttcg tgtggttttt gtcttttggt
     1561 cagctccttg gcgcatattg catctacctg ctcttctttc gcgcggacgt ttcccctttg
     1621 attgctcacg tagtcgaaat tggttgggcc ggcggaccag gatctgcggc ggccatgtct
     1681 gccgttatgc ggcgactcgg ccagtctgcg attggggacc tgggactttt ctctgctact
     1741 cttggacagg tctggggagc actcagcgga atctggattg taaatcatct aaacagaaaa
     1801 gctcagcacg taattggaac agaagcaagc tcttcgcacg aatcagcgcg acctctgtcg
     1861 tccgtagaag caaatggaat cgcggacgcg gaagtgcgcc gtccacgctt cccaggaccg
     1921 aaaggcctac tgcgcatctt cctattgttc cttcttccgc tttccgctgt gttcattgcg
     1981 atggccgcaa aagaagggct gggaatcgtc gccatttaca ctccgcgcgc actcttcctt
     2041 ggagacattc ccctcttttc tcttgcgcta ctgtgcgcat tccccgtcag gtggatcgcc
     2101 atccgcgctg gaatcctgac tgctgaaaac tgcgaaacga tccagaacat aaacggccag
     2161 gtcctggact ggatcatcct ggccgctctc gcagcacttg aacccgcagc cttcattgca
     2221 cttcttgttc cattctcagc gatgatggca ttcggtgctt tcttctgctt tgttctgatg
     2281 tatttcttcg cgccgcgaat gctgcccaca aatacgtgga gagagcttgg actgatcaac
     2341 tacggaatgg ccaccgggac cacagctctt ggtttgatgc ttcttggatt gttgcgttcg
     2401 cacacgaaag acgaagcgag acttgtatac ggcatggcag ttccgttcaa cgccccattc
     2461 gtcggaggtg gaattctttc tctcactctt ccagagtgga ctgcgcgcgg aggcgaaaca
     2521 atcattcctt tcttgctcct tgcgtggatg tttgttttct ttgtagtgtg cagacgattg
     2581 caacagaagg aagcccatga gtaagatgcc tgttttgttt gtagggcatg gaaatccaat
     2641 gctcgtcctt gaggacaatg agatcacgca agcctgggct acgctcgggc agagcctgaa
     2701 acctcgcgcc attctagcgg tttccgcgca ctgggtcaca cgcggaacgc tcgtgacctc
     2761 catgcagaag ccccgcatca tatatgattt ttatggattt cctgatgaac tatatcaggt
     2821 gacttatcct gcacccggct cacctgagct tgctcttcgc acagcatctt tgactg
//
LOCUS       DAOQJL010000506         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_23941_length_2864_cov_3.402990, whole genome shotgun sequence.
ACCESSION   DAOQJL010000506 DAOQJL010000000
VERSION     DAOQJL010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23941_length_2864_cov_3.402990"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1021)
                     /locus_tag="PLG78_17045"
     CDS             complement(161..1021)
                     /locus_tag="PLG78_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE24882.1"
                     /translation="MESFLELQGVRVGFPIQKGIFRRTHGHKIVLDSVSFKIARGKTV
                     GLVGESGSGKTTIGRAILGLVPVMSGSIPLHLDDPLISREPLDLAHGFPDDFDPSTGQ
                     VRSLSRMLRRRLQIIFQDPYSSLNPRLRIGDVLLEAMHLHFPEMTEALRRKKAKDTLD
                     RVGLSDSALERYPHEFSGGQRQRISIARALVLNPEFIVCDECVSALDVSIQAQILNLL
                     RDLQEERRLTYLFISHDLSVVRYISHEICVLDRGTIVEQGDAARVVDHPQQPYTKRLL
                     EAVPSLIPRR"
     gene            complement(1032..1385)
                     /locus_tag="PLG78_17050"
     CDS             complement(1032..1385)
                     /locus_tag="PLG78_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNE24883.1"
                     /translation="MAKSASKFSFLDVLQEGILEIAEAGKNGTVKLKRSDLKALIEKA
                     FQQGVKAAAGGERVKFPVVGTLLRKDVEAKKGGKGRNPFTGEEIMIKPRPATKKPRWS
                     FPKSIKEDFANKKNW"
     gene            complement(1437..1826)
                     /locus_tag="PLG78_17055"
     CDS             complement(1437..1826)
                     /locus_tag="PLG78_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24884.1"
                     /translation="MTIGIVPARESEVFRIQGPFRLVLEDTDRPNVKKLKHVIRENPQ
                     TVSDFKHFIHYLTGQIRRAADLLAEVGDDRHKRRAEALRNHAMEELQIAGRYENDPDR
                     LRACMMRLSSTLIDLKQVANEEEVQGH"
     gene            complement(1890..>2864)
                     /gene="purT"
                     /locus_tag="PLG78_17060"
     CDS             complement(1890..>2864)
                     /gene="purT"
                     /locus_tag="PLG78_17060"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNE24885.1"
                     /translation="DLILKVKPSLIVPEIEAIATAALVRLESEGFTVIPTARAARLTM
                     DREGIRRFAAEELGLPVSRYRFAGTQAEYEDAVREIGMPCVIKPVMSSSGKGQSTVRK
                     EEDVAKAWQYAMTGGRAKGGRVIVEEWIPFDYEITLLTVRHSGGTDFMNPIGHLQIDG
                     DYRESWTPQPMSEKALTAAQSIAKSITDGLGGRGIFGVELFVKGDQVYFSEVSPRPHD
                     TGLVTLISQNRSEFALHARAILGLPIGTIHSYGPAASCALLMEGDSDAPVLEGLEDAL
                     NGADTDFRLFGKPEIHGKRRLGVALALGQTVEKAREAARNSAASIRLL"
BASE COUNT          644 a          810 c          717 g          693 t
ORIGIN      
        1 tctcgcccac ccctacatcc gagacttgat tctcagctgc cgcagcccct ggcctgccca
       61 actgcgcaga caggaagtcg aagccccggt gcgagtcggg caaggacgcc cgcgtctcgc
      121 accggttggg cgggtcaggg tctgcgggaa gcaggtgaaa tcaacgtctc ggaatgagtg
      181 aaggcaccgc ttccagcagc cgctttgtat aaggctgctg tggatgatcc accacgcggg
      241 ccgcatcacc ctgctctaca attgtcccgc gatccagaac acagatttcg tgtgaaatgt
      301 aacgtactac agataaatcg tgagagatga acaaatacgt tagacggcgt tcttcctgta
      361 gatcacgaag caggtttaag atctgcgcct gaatagacac gtccagcgcg ctgacacatt
      421 cgtcgcacac aatgaactca ggattcagaa ccagagcgcg cgcgatgctg attctctgtc
      481 tctggcctcc gctgaattca tgagggtagc gctccagagc cgaatcagaa agtcccactc
      541 tgtccagagt gtctttcgcc ttttttctcc gcaacgcttc tgtcatttct ggaaaatgca
      601 aatgcattgc ttctagcagt acgtctccga tgcgaaggcg cggattcaaa ctggaataag
      661 gatcttgaaa aataatctgc aatctgcgcc gaagcatgcg cgaaagagaa cgaacctgtc
      721 cggtagatgg atcaaagtcg tctggaaaac cgtgggcgag atccagtggt tccctggaga
      781 tgagaggatc atccagatga agcggaatgg aaccggacat cacaggcaca agcccgagga
      841 ttgcacgtcc gatggtagtc ttgccggaac ctgattcacc caccagaccg acggtcttgc
      901 cgcgggctat tttgaacgaa acagagtcga gtacgatctt atggccatga gtccttcgaa
      961 agataccttt ctggattggg aacccgactc gaacgccttg caattcgagg aaggactcca
     1021 tggcttttgg attaccagtt cttcttgttt gcgaagtctt ctttgataga cttcgggaaa
     1081 gaccatcttg gtttcttggt tgcagggcgc ggcttgatca tgatctcttc gcccgtgaaa
     1141 ggattacggc ccttgccgcc tttctttgct tccacgtcct tgcgaaggag agtaccgact
     1201 accgggaact tcacacgctc gccgccggcg gctgctttga ctccctgctg gaatgctttt
     1261 tcgatcagcg ctttgagatc actgcgcttc aatttcactg tgccgttttt tccggcttct
     1321 gcgatttcaa gaatgccttc ctggagaaca tcaagaaaag agaatttgct agctgatttg
     1381 gccatatcct gttgtttctt tttattggaa gatatcctgc aagtaaaatt tcaaaatcaa
     1441 tgaccctgca cttcttcttc gttcgctacc tgctttagat cgatcaaagt agaactcaat
     1501 ctcatcatgc atgcgcgaag gcgatctgga tcattctcgt aacgcccggc tatctgcagt
     1561 tcctccatcg catgattgcg gagtgcttct gctcgtcttt tgtggcgatc atccccaact
     1621 tcagccaaaa ggtccgcggc ccgcctgatc tgacccgtta gataatggat gaaatgctta
     1681 aaatcggaaa cggtctgggg attctcccgg atgacgtgct tgagcttctt tacatttggc
     1741 cgatccgtgt cttccaggac caggcggaat ggtccctgta tgcggaaaac ctcgctttcg
     1801 cgagcaggca cgattccgat cgtcatattc cgtccccggg acttcatcga ctcacttctg
     1861 actctggccc gggcgttcac aagacaaaat cataggagtc gaatgcttgc ggccgaattt
     1921 ctcgccgcct cacgcgcctt ctctacagtc tggcccaggg caagcgccac acccaggcgc
     1981 ctctttccgt ggatctcagg ctttccaaaa agtctaaaat ccgtatctgc tccgttcaaa
     2041 gcatcctcca ggccttccag aaccggggca tccgaatctc cttccataag caaggcgcaa
     2101 gatgcggccg gaccatacga atgaattgtg ccgatcggca ggccaaggat ggcgcgggca
     2161 tgcagtgcga actcggaacg attctgcgaa atcaaagtta caagacccgt gtcatgcggt
     2221 ctgggagaaa cttcgctgaa atagacctga tcccctttta caaaaagctc cacgccaaag
     2281 atgccccgcc ctcccagacc gtccgtgatg gacttcgcaa tcgactgcgc cgcagtcaga
     2341 gccttctcgc tcattggttg cggcgtccac gattcacgat agtcgccatc tatctgtagg
     2401 tgaccgatcg ggttcatgaa atccgtcccg cctgaatggc gcacggtcag tagagtgatt
     2461 tcgtaatcaa aggggatcca ttcctcaaca atgacgcgtc cgcctttcgc acgccctcct
     2521 gtcatcgcat actgccaggc cttcgctaca tcctcctctt ttcttacggt gctctggcct
     2581 tttccggagg agctcatgac tggcttgatc acgcagggca ttccgatttc gcgcactgca
     2641 tcttcgtatt cagcttgcgt cccggcgaaa cgataacgcg aaactggcag gccaagctct
     2701 tctgcggcga atcgacgaat gccttcgcgg tccatagtca ggcgagccgc acgcgccgtt
     2761 gggatgactg tgaacccttc gctttcgaga cgcacaagtg cggctgttgc aatcgcctca
     2821 atttcaggta caatgagaga aggctttact ttcaagatca gatc
//
LOCUS       DAOQJL010000507         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24013_length_2858_cov_4.140920, whole genome shotgun sequence.
ACCESSION   DAOQJL010000507 DAOQJL010000000
VERSION     DAOQJL010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24013_length_2858_cov_4.140920"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PLG78_17065"
     CDS             <1..1474
                     /locus_tag="PLG78_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE24886.1"
                     /translation="VTSSRVKSTLEAIAQKAGKEDVVAIYFSGHGTYQRDASAPNGLR
                     NYIVMYDRPHVSDKELSDWIKGIKTQHVTLIMDCCYSGGIAKKGRRGVGDIPTSPGSD
                     GTVIQNGDENFYFQNKAIIASSDSNETSIEVRGSINHGVFTYWFAQGLDPRNGDLNKD
                     GTVTALEAFEWSSRRVADMAKKQFNHDQNPQISGNAKGIFLAGREVPTPQPPVVTPVV
                     NPPQPDPNKPQPPQPPQPPVVTPVTPVTPTPPNVEPPPENPNTNDTGKVIIATTILRS
                     QLSGTQTPDPAMMMRRMRGVDSDRTVKVRFSGKELPATVTWVDQAGLKAATGEDIPLG
                     TYTVGRQGTPTVVPNTVALIEVNGVPGGVHEVEIISEDYPVIKETIGVEGKNVVKKFV
                     VASLRGTGSIQGRVFLKNFEQPLPGQTIWMPTVVGTNQVHKMKSLTDGSFWFMNLPPG
                     NYYEIKASFEENLKLDNQYIKVKDGDVTKVDIVLSQKNFK"
     gene            1501..1701
                     /locus_tag="PLG78_17070"
     CDS             1501..1701
                     /locus_tag="PLG78_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24887.1"
                     /translation="MKLIFLALVITILYQMSTQAMARVNSRASGEGNQQKSPGERKPL
                     LSREKKPTVEKDITHRGRVLRD"
     gene            1755..>2858
                     /locus_tag="PLG78_17075"
     CDS             1755..>2858
                     /locus_tag="PLG78_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24888.1"
                     /translation="MRMRALAILALLLGACAPYQTRNDFQSARRDVSRGGPQASKIAN
                     DILANLNDIPPAMVGEALDTLIAIPDPEGERAVLEASTRPQFQAPEYREKILRRLLGS
                     DSQQARDFVLKEIKAKPDLMNDPTILSWVVAKDPPGASELVLAHVQKNPASLNEQTIN
                     YFGTKRYTPSIPVLLSSIDSGKNADLCLKTLAQMGDPKADAAILSIAQKESPVQMRAI
                     QALPEMKDPDRRKEAESVLEGMWKNNPPGEMKTTALTGLAKMRGQDPLELEREAAFPM
                     ANVQGDLAVNSLEDRAAKRDMISPEPVKPAVTPVQPVVTQPTVVPTRPVQPSQPQVVQ
                     PAQPSKPVVTAPRNEPAHTQPAKPDAKKDQVAVR"
BASE COUNT          778 a          765 c          733 g          582 t
ORIGIN      
        1 ggtcacatct tcgcgcgtca aatccactct agaagcaatc gcgcagaagg cagggaagga
       61 agacgttgtg gcgatttact tttccggtca tggcacatac cagcgtgacg ccagtgcgcc
      121 caacgggctt cgcaactaca ttgtaatgta tgatcgcccg cacgtttctg ataaggagct
      181 gagcgattgg atcaaaggca ttaagacaca gcacgtaact ctgatcatgg actgctgcta
      241 ctcaggcggc attgcgaaga aaggtcgtcg cggagtcggg gatattccta cttctccagg
      301 atcggacgga accgtgattc aaaacggaga cgaaaacttc tattttcaga acaaagcgat
      361 catcgcttcc agtgattcaa acgaaacgtc tattgaagtt cgcggaagca tcaatcatgg
      421 agtattcacg tactggttcg cccagggtct cgatccgcgg aacggagact tgaacaaaga
      481 cggcaccgta accgcacttg aagcatttga atggtcttcg cgccgagtcg cagacatggc
      541 aaagaagcaa ttcaatcacg atcagaatcc gcagatttcc ggaaatgcaa agggaatttt
      601 cctcgcgggt agagaagttc ccacaccgca gccacccgtt gtgactcctg ttgtgaaccc
      661 accgcagccg gatccaaaca agccacaacc gccccagcca ccgcaaccac cagttgtgac
      721 tcctgtgact cctgtgactc caacgccgcc taacgtggaa ccaccaccgg aaaatccgaa
      781 tacaaacgac actggcaaag taatcatcgc cacaaccatt ctccgcagcc agctttcagg
      841 aacgcaaacg ccggatccag ccatgatgat gagaaggatg cgcggtgtgg attcagatcg
      901 cactgtgaaa gttcgcttct ctggaaaaga acttcccgca actgtaacct gggtggacca
      961 ggccggtctc aaggcggcca caggagaaga cattcctctt ggaacttata cagtgggtcg
     1021 ccagggaact cctaccgttg ttccaaacac agtggcactc attgaagtca acggtgtgcc
     1081 aggcggagtt catgaagtag agatcatctc cgaagattat cctgtgatca aagagaccat
     1141 tggcgtagaa ggtaagaatg tagtgaagaa gttcgtggta gcttctctgc gcggaactgg
     1201 ctctatccag ggccgggtct tcctgaagaa tttcgaacag cctcttcctg gccagactat
     1261 ctggatgcct acagtcgtgg gcaccaacca ggtgcacaag atgaagtcgc tcacggacgg
     1321 tagcttctgg ttcatgaatc tgcctccggg caactactat gaaatcaagg cttcctttga
     1381 ggaaaacctg aagctggaca accagtacat caaggtgaaa gatggtgacg tgacaaaggt
     1441 ggatatcgtt ttgtctcaga agaacttcaa atagggcgca aaagcgccct ccaggtgtga
     1501 atgaagctaa tttttctggc tctagtcatt acgattctat accagatgag cacccaggca
     1561 atggcccgcg tgaattcgcg ggcttccggg gaaggcaatc agcagaaatc gccgggagag
     1621 cgcaaaccac tcctttccag agaaaagaag cccaccgtag aaaaagacat cacccaccgc
     1681 ggccgcgtcc tcagagactg accttttttt tcctgaattc gtctaatcaa gttagaccga
     1741 taataggaac agctatgaga atgcgcgccc tggccatttt ggccttacta ctcggagcat
     1801 gcgctcccta tcagaccaga aacgattttc aatctgctcg cagagatgtt agtcgggggg
     1861 ggccccaggc gagcaagatc gccaatgata ttctggcgaa tctaaacgac atacctccag
     1921 ccatggtagg agaggcgctc gatactctca tcgctattcc agatccggaa ggtgagcgcg
     1981 ccgtactgga agcttccacc cggccgcagt tccaggcgcc ggagtatcgc gagaagatac
     2041 tgcgcagact tctgggttcc gattctcagc aggcgcgcga cttcgtgctg aaagaaatca
     2101 aggcaaagcc ggatctgatg aatgatccaa ccatcctctc ctgggtggtt gcgaaagatc
     2161 ctccgggtgc atctgaactc gtgctggctc acgtacaaaa aaatccagct tccttgaatg
     2221 aacagaccat caactatttc ggaacgaaac gttatacgcc atctataccg gtgttgcttt
     2281 cctccattga cagtggaaag aatgcggatc tctgtctgaa gactctggct caaatgggag
     2341 acccaaaagc ggacgcggcc attttgtcta tcgcacaaaa agaaagccca gtgcaaatgc
     2401 gcgccattca ggcgttgcca gaaatgaaag atccagatcg cagaaaagag gcggaaagcg
     2461 ttctcgaggg aatgtggaaa aataatccgc ccggagaaat gaaaaccact gcattgactg
     2521 gacttgcgaa gatgagaggc caggacccac tggaattgga aagggaagct gccttcccta
     2581 tggccaacgt gcagggtgac cttgcagtga attctctcga agatcgcgcg gcaaagcgcg
     2641 atatgatatc tcccgaacca gtgaagccgg ccgtaacacc ggtgcaacct gtggtcactc
     2701 aacccacagt cgttccaaca agacccgttc aaccctcgca gccgcaggtc gttcagccag
     2761 ctcaaccttc aaaaccggtg gtaactgctc cgcgcaatga gcctgcgcat acgcagcctg
     2821 caaagccgga cgcaaagaaa gatcaggtcg ctgttcgc
//
LOCUS       DAOQJL010000508         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24073_length_2854_cov_2.854948, whole genome shotgun sequence.
ACCESSION   DAOQJL010000508 DAOQJL010000000
VERSION     DAOQJL010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24073_length_2854_cov_2.854948"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PLG78_17080"
     CDS             complement(<1..1259)
                     /locus_tag="PLG78_17080"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24889.1"
                     /translation="MKKITFRDRMRYRFDNLMSRGSIALIIYLGLLTVALIVGVTIID
                     IAGDLAPADDKGEKPGFSELLWKSLLRTLDPGTMGGDTGDWGFLFSMFVVTIGGIFIV
                     STLVGILATGIDQKLEDLRKGKSLVVEKDHTLILGWSENIYTIISEICEANRSRGKSC
                     IVILADKDKVEMDDEISSRIENPGKTRIVTRSGNPIDFVNLEIANPHSSRSIVVLSGD
                     GADPDAQVIKTVLAIINNPGRRKEPYHVVAELKRPESVQICEMIGKDEVKTVLSPEVI
                     SRIMVQTCRQPGLSVVYTELLDFGGDEVYIHEEASMAGKTYADALLAYPKASVMGLVK
                     SGGKIFLNPSSTEKIESGDRLIAIAEDDDKILLGSGTNYGIDSGAIAKSDRAKPAVEN
                     TLLLGWNDRVGMMLSELDQYVSQGSSLT"
     gene            1371..1601
                     /locus_tag="PLG78_17085"
     CDS             1371..1601
                     /locus_tag="PLG78_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNE24890.1"
                     /translation="MADPKHRQPENAPGKYYVDDTCVPCHVCVDEAPGLIQYAADEGH
                     VFFARQPVTPEEIRAAENAMKGCPTEAIGNDG"
     gene            complement(1623..>2854)
                     /locus_tag="PLG78_17090"
     CDS             complement(1623..>2854)
                     /locus_tag="PLG78_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE24891.1"
                     /translation="DFPKDVQIKETEVLRAKMELAPRHTSRQEITGDVKKLAEALNKA
                     ERPLLYVGGGAINSGASKEIKALAEKAMAPVTTTLMGLGAFPGTHILSVGMLGMHGTA
                     TANKAVLECDFLLSLGARYDDRVAGQADDFARDAVRAHIDIDASEFNKRVSVDHLVCG
                     DLKDVIQAILPYVEKKDRSPWVANTESLKQKNPLAYEESEEVIKPQRILKRLFEVTKG
                     RAIVSTDVGQHQMWAAQYYQFDEPNRWLTSGGLGTMGYGLPAAIGAKFAKPDDLVVCV
                     TGDGSYQMCIQELATIRQYNLGVKILLLNNNFLGMVRQWQELFYEERFAESEWDYNPD
                     FVKLAEAYGIPGMRITSPDEIDSGLEFFLKDNGPAFLEALIPADEKVFPMIAAGKSQR
                     DMLQFEDLKRMMQARQK"
BASE COUNT          682 a          730 c          676 g          766 t
ORIGIN      
        1 gtgagggaag aaccctggct tacatactga tccagttcag aaagcatcat tcccactcga
       61 tcgttccatc ccagaagcag agtgttttcc acggccggtt ttgcacggtc cgatttcgca
      121 atggcaccgg aatctattcc ataatttgtg cctgatccaa gcagaatctt gtcatcatct
      181 tctgcaatgg caatcaagcg atctcccgat tctatcttct cagtggacga aggattgaga
      241 aaaatcttcc cgccgctttt cacaagaccc atcacagatg ctttcggata ggccaaaaga
      301 gcatctgcgt aggtttttcc tgccattgat gcttcttcgt gaatgtacac ttcgtctcca
      361 ccgaagtcca atagctccgt gtatacaacg gaaagcccgg gctgtctgca agtctggacc
      421 atgattctag agatgacttc tggagagaga acagtcttga cttcgtcttt accgatcatt
      481 tcgcagatct gcactgattc aggtcttttt agttccgcca ccacatgata gggttctttc
      541 cttcttcctg gattgttgat gatagcaaga actgttttga tgacctgagc gtccggatct
      601 gctccgtctc ccgacaaaac tacaatgcta cgagatgaat gaggatttgc gatttcaaga
      661 tttacaaagt cgattggatt tccgcttcgc gtaacgattc tggttttgcc gggattttca
      721 attctagatg aaatctcatc gtccatttct actttgtctt tgtctgctag aatcacaatg
      781 cacgatttgc cgcgagagcg attggcttca cagatctcag atatgatggt gtaaatgttt
      841 tcggaccatc caaggatcag agtgtgatct ttctcaacta ccagagactt acctttgcga
      901 agatcttcca gcttttgatc aatgcccgta gcaagaatgc ccaccagcgt gcttacaata
      961 aaaatgccac caatggtgac tacgaacatg gagaagagaa agccccagtc ccctgtgtct
     1021 ccgcccatgg tgcccgggtc cagtgtgcga agtaggctct tccacaacag ttcgctaaag
     1081 cctggctttt cgcctttgtc atctgccggg gcaagatctc cggcgatatc aatgatggtc
     1141 acgccaacga tgagcgccac tgtgagcaag cccaggtata tgatgagggc aatggaccct
     1201 ctggacatga gattgtcgaa acggtagcgc attctgtcgc ggaatgtgat ttttttcatc
     1261 tgtgtagtgg acccctctcg gcagtctctg gcgcaggttt cttttcgcgc aatctatttt
     1321 ggaagaatca aagtatttga caggcgtagg gtaaaaagtg cgcttccaac atggctgatc
     1381 caaagcacag gcagccggaa aatgctccgg gcaaatatta cgtggatgat acctgcgttc
     1441 cctgtcacgt ctgcgtggat gaagctcctg gactcatcca gtatgcagcg gacgaaggtc
     1501 atgtgttctt cgcacggcaa cctgttacgc cggaggaaat ccgcgcggct gaaaacgcaa
     1561 tgaaaggctg cccaacagaa gcaatcggaa acgatggctg agtaaaatcc tcagccattc
     1621 cctcatttct gtctggcctg catcatgcgc tttaagtctt cgaattgaag catatcgcgc
     1681 tggctttttc ctgccgcaat catggggaaa actttttcgt ctgcaggaat caacgcttcc
     1741 aggaatgcag gaccgttgtc tttgaggaaa aactcaaggc ctgaatcgat ctcatccggg
     1801 gaagtaattc gcatgccagg aattccgtat gcttccgcaa gcttgacgaa atctggattg
     1861 taatcccatt cagattcagc gaatctttct tcatagaaga gttcctgcca ctgacgtacc
     1921 atccccagga aattgttgtt caacaacaaa atcttcacgc ccaggttgta ctgtcgaatg
     1981 gtcgcaagtt cctgaatgca catctggtag gatccatctc ctgttacgca aacgaccaga
     2041 tcgtctggct ttgcgaactt ggctccaatg gcggcaggca gtccatatcc catagtgccc
     2101 aggccaccgc ttgtgagcca gcggtttggt tcatcgaatt gatagtactg cgccgcccac
     2161 atctggtgct ggcctacgtc cgtagaaacg atggcacggc ccttcgtaac ttcgaagagc
     2221 cttttgagaa tgcgctgggg cttaatgact tcttcgcttt cttcatacgc gaggggattc
     2281 ttttgtttga gtgattctgt gttcgcaacc cacggactgc gatccttctt ttctacgtag
     2341 ggcagaattg cctgaatcac gtctttgaga tctccgcaaa ccagatggtc cacagaaact
     2401 ctcttgttga attcagaagc atcaatgtcg atgtgagcgc gcactgcatc gcgagcgaaa
     2461 tcgtctgcct gacctgcgac tctatcatca taacgagcgc caagagacag aagaaaatca
     2521 cattcgagca cagccttgtt tgcagttgca gtaccgtgca ttccaagcat gccaacagaa
     2581 aggatgtgag ttccaggaaa cgcgcccagc cccatcagag tagtggtgac aggagccatt
     2641 gctttttctg cgagtgcttt gatttctttg gatgctccgg agttgatcgc accgccgccc
     2701 acataaagca gaggtcgctc cgctttgttc agagcctctg cgagcttttt tacatctccg
     2761 gtaatttcct ggcgactcgt gtgacgcgga gcaagttcca tcttcgcgcg aagcacttcc
     2821 gtttctttga tttgaacatc tttggggaaa tcaa
//
LOCUS       DAOQJL010000509         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24177_length_2846_cov_3.766034, whole genome shotgun sequence.
ACCESSION   DAOQJL010000509 DAOQJL010000000
VERSION     DAOQJL010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24177_length_2846_cov_3.766034"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLG78_17095"
     CDS             complement(<1..558)
                     /locus_tag="PLG78_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24892.1"
                     /translation="MKTRLAVGSFLVACAIFAILRNAEADPAILSETAYPSVAWKDFS
                     PTIDLSSPPALRQTESIDLWDGMIFAKDRVVLPLRTAGGRVLWSWEPGKLPVRSEIQG
                     DIQSIAYDDGKDEILILTTNKLLFLRRSSLAILRETPTSIQASDMGVSGKSLIARKGN
                     DFTVYSLPDLQKLRTFSAPQPQFTQN"
     gene            complement(594..1460)
                     /locus_tag="PLG78_17100"
     CDS             complement(594..1460)
                     /locus_tag="PLG78_17100"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE24893.1"
                     /translation="MRLCVFLLCLFLTTCAAAEPADSHDPLAAVIEVSRKYQVLPNNT
                     YRVVQGRELKLDVFRPRKDGPLLPVMVYIHGGGWVSHDKERATLHTLPFLARDHVVFN
                     IDYRVGKGTAPAGVEDCFCALKWIRAHAAEFRADGSSVVVMGHSAGGHLALMMGVGKD
                     NGACPGPLPVVRAVVNWFGISDVRDVYEGANSQQYAREWLEGKLDPRALADSVSPIRL
                     VRADNPPIISIHGDHDPAVPYPQSVRLHEALSKAHVKNQLITIKGGTHGGFPEPEYLR
                     AYRAVFAFIGIP"
     gene            1628..2035
                     /locus_tag="PLG78_17105"
     CDS             1628..2035
                     /locus_tag="PLG78_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24894.1"
                     /translation="MRFSVFFLTALFLVFLPAAILSFFMHHPDTWTEESVVGFALAVV
                     AFGLIPNGIPALLLVFLMIWKKVASVRDFVQRRAVLCSFLAALASVLVIHWWTFADPA
                     EAGLLIHSLAVVFFGGIAVLFGFICGSWIEKKS"
     gene            2050..2553
                     /locus_tag="PLG78_17110"
     CDS             2050..2553
                     /locus_tag="PLG78_17110"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNE24895.1"
                     /translation="MSVPLPPSSVLYAPSIPYDYADSYSAVLSDSSMTATDAAWEFFG
                     QAPRWVGTLMRMRNSLVSLFGLKTGQSQKPVERRALAPGQKVGLFRIYSASPTEVILG
                     EDDRHLNFRVSVLVEPGDQGKKNLVVSTVVHYQNRWGQMYFFFVRPFHRIIVPAMFRR
                     MITRLAA"
BASE COUNT          600 a          727 c          783 g          736 t
ORIGIN      
        1 gttttgtgtg aattgcggct gtggagcgga gaatgtacgt agtttctgaa gatcgggaag
       61 agaatagact gtgaaatcat ttcctttcct tgcgatcaaa gacttgccag atacccccat
      121 gtctgaggct tgaatgctgg tcggagtttc gcgcagtatg gccagactgg atctgcgaag
      181 aaacaatagt ttattcgtgg taagtattag aatctcatct ttgccgtcgt cataggcgat
      241 gctttgtata tctccctgaa tttcagagcg gactggaagc ttgcctggct cccaggacca
      301 cagcacgcgt ccgcctgcag ttctgagtgg aagcacaacg cgatcttttg cgaagatcat
      361 accatcccag agatctatag actcagtttg tctgagtgcc gggggagagg acaagtcgat
      421 ggtgggagaa aagtccttcc acgcaacaga aggataggcc gtctctgata ggatcgccgg
      481 gtctgcctct gcattcctga ggatcgcgaa aatcgcacat gcaactagaa aagatccgac
      541 ggccagtctg gttttcattt gagcctcgaa ttgtagaatt ttgcgaagta ctgtcaggga
      601 atgcctatga acgcaaacac tgccctgtag gcgcgaaggt attcaggttc tggaaaaccg
      661 ccgtgagttc cacccttgat tgtgatcagc tggtttttca cgtgcgcctt gctcaacgct
      721 tcatgaagac gaacgctttg cggataggga acggcggggt catgatctcc gtgaatggag
      781 atgatggggg gattgtccgc cctcaccagg cgaatgggcg atacgctgtc tgcgagagcg
      841 cgcgggtcca gcttaccctc gagccattcg cgcgcgtatt gctggctatt tgctccctcg
      901 tacacatcgc gaacgtctga gatcccaaac cagtttacca ctgcgcgaac tacaggcagc
      961 ggccctggac aggcaccgtt gtctttgccc actcccatca tcagtgccag gtgtcctccc
     1021 gcagaatgac ccatgaccac tacacttgaa ccatctgcgc gaaactcagc ggcatgcgcg
     1081 cggatccatt tcaaagcgca aaaacagtct tctactcccg caggtgcggt tccctttccc
     1141 acgcggtaat cgatattgaa tacaacatga tcgcgtgcca gaaaaggaag cgtatggagg
     1201 gttgctcttt ctttgtcatg agagacccag ccaccgccgt ggatgtagac catgacaggg
     1261 agaagagggc cgtctttgcg cggacgaaat acatccagct tcaattcgcg gccctgaacc
     1321 actcgatacg tattgtttgg aagaacctga tacttcctgg atacttcgat gacagctgcg
     1381 agtggatcgt gagagtccgc gggctccgcc gcagcacagg ttgtaagaaa caggcagagt
     1441 agaaatacac acagtcgcat gagcccacgt aggcgcagac agattgtctg tcacgcgaaa
     1501 ctccgggccc ggatgcttgc gaatccgagg gtcgggggtt tccgtctccg gtatgccgca
     1561 taacgttacc cctttccatg ggcgaatttt ttggttgcct gcatcttctc agggggaagg
     1621 ctgaggcttg cgcttctctg tattttttct gaccgccctt tttctggttt ttttgcctgc
     1681 ggccattctt tctttcttca tgcatcaccc ggatacatgg actgaggaga gcgtcgttgg
     1741 ctttgcgctg gcagtggttg cgttcgggct cattcccaac ggaattcctg ctctgcttct
     1801 tgtcttcttg atgatctgga agaaagttgc gagcgttcgc gactttgtgc agagaagggc
     1861 agtgctttgt tctttcctgg ctgctctagc ttctgtcctg gtgatccact ggtggacctt
     1921 tgcagatccg gcggaggcgg ggctgttgat tcactcattg gcagtggtgt tcttcggagg
     1981 gatcgctgtt ctatttggat tcatctgtgg ttcctggata gagaagaaat catgacgagc
     2041 gggaaaatta tgtccgtacc tctgccccct tcttctgttc tctatgctcc gagtatccca
     2101 tacgactatg cagacagcta ttcggctgtt ctttctgatt cgtctatgac tgccacggat
     2161 gcggcgtggg agttttttgg tcaggctccg cgatgggtgg gaactctcat gcgcatgcgc
     2221 aattcccttg tttccctttt cgggctcaaa actggtcaga gtcagaagcc ggtagagaga
     2281 cgagctctcg cgcccggaca aaaagtgggg ctctttcgca tctattcggc cagtcccacg
     2341 gaagtgatcc tcggggaaga tgatcgtcat ttgaattttc gtgtctctgt cctggtagag
     2401 ccaggagatc agggtaaaaa aaatctggtt gtgtctactg tagtgcatta ccagaaccgg
     2461 tggggacaaa tgtatttttt cttcgtgcgt ccgttccatc gcatcattgt tcccgccatg
     2521 tttcgtcgca tgatcactcg attggccgca taacgttgca gtggaaagcg ccagcgatca
     2581 agcgggcggc ctgcgttcgt ttcacttccg aattgttgtg aggctgatcg caccaaaaat
     2641 tctggccggc ccgtgcgagc gcggtcctga ccgcagggca ggaggcgccc cgaactcttc
     2701 tttgcacgaa atgagacgag acataatgcg ggaggtcctc tcgagcgcgt ggatggtttt
     2761 aagccttgac cgtaaaaact ttcgcggcca tgtgttccta tgaaactacc tcaaaaactg
     2821 gccctgtcca atgtgcggcg tactcc
//
LOCUS       DAOQJL010000510         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24344_length_2834_cov_5.049298, whole genome shotgun sequence.
ACCESSION   DAOQJL010000510 DAOQJL010000000
VERSION     DAOQJL010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24344_length_2834_cov_5.049298"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PLG78_17115"
     CDS             <1..1425
                     /locus_tag="PLG78_17115"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE24896.1"
                     /translation="ARLRSVLRPAKAVAVPDRLSFYPPEIAAKGNAVEKIRHHLCHVA
                     TAAFGSNIGDGHDLLIVTVDGLGDGECATVNVLRADGQIEPQLSVHQAHSFAQIYAFV
                     TVYLGFVHLEHEYKLMGMAPYGRDRDAERIADKLEGLFTYTDARWELKTPVWNLNSDE
                     RLVYESLREMFEFERFDNICAGLQLFSERFIVRFIQDWIRKTGVRRVAVSGGFFMNVK
                     ANQKLRSLPEIEQLFVYPSCGDETNAMGAAYLKYFQLTGKRPEPLKNFTLGPVIDDST
                     LLDVLKSYGEKIRFEYCEDIEERAADLLSKKNIVARVKGPEEFGARALGNRSILAHPS
                     DTLAVRTINDMIKSRDFWMPFACSILEEEKLRYLKDWTANFSPNYMTMTYDSKNSEEI
                     LAGMHPRDRTVRPQVVTREQNADYHRLLSRFHDMTGIGGVLNTSYNLHGFPLVHDASA
                     AIEVFLNSGLEYLALNQYLVTKPG"
     gene            1429..2124
                     /locus_tag="PLG78_17120"
     CDS             1429..2124
                     /locus_tag="PLG78_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24897.1"
                     /translation="MISRLKQFIGGLLHRYRLGQEARRHRKEVEGKSVDVVFTEIFKR
                     NFWGDRESVSGSGSNQEQTRVIVREIPRVLKQVGARTLLDLPCGDFNWMQRVDLSGVS
                     YTGGEIVQDLVDLNQKKFGRDGISFKKMNLIDGDLPASDVLLCRDCLVHLSNEQIHAS
                     LANIKKHGIRYLLTTSFTETKKNSNIVTGEWRPINLEIPPFSLKAVEVINEECTEGNG
                     QFADKSLILVRMN"
     gene            2138..2572
                     /locus_tag="PLG78_17125"
     CDS             2138..2572
                     /locus_tag="PLG78_17125"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HNE24898.1"
                     /translation="MIQSKKNPRIPTKNHDGKQNGFLIPIINVNEGFPSPDAWPKQVY
                     CTVAYPGQVKGPHLHKKRAGLFTCIKGNVKIVIRIDGKYVEHFSGDDHEYATIQVPAG
                     IPSALVNIGKEDAYILNMPSPAWSKDEPDDWDVSFEGYNFNA"
     gene            2569..>2834
                     /locus_tag="PLG78_17130"
     CDS             2569..>2834
                     /locus_tag="PLG78_17130"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE24899.1"
                     /translation="MNRTLLITGGAGFIGSHLILRLFERYPDYRFVNIDKLTYASDES
                     YLDPIRSSGRYTFAKVDLVNKADVTAVFEEHKPDGIIHLAAETHV"
BASE COUNT          732 a          666 c          713 g          723 t
ORIGIN      
        1 gcgaggcttc gcagtgtctt gcgtcccgcg aaagctgtgg ctgtgccgga tcgtctcagc
       61 ttctatccac cggaaatagc agcaaaggga aatgccgtag aaaaaattcg ccaccatctg
      121 tgtcacgtgg ctacggctgc gtttgggtca aatattgggg atgggcatga tctactcatt
      181 gttacagtag atggtctcgg agatggtgaa tgcgccacgg tgaacgtact gcgcgcggac
      241 ggacagatcg agccacagct cagcgttcat caggcccatt ccttcgcaca aatatacgcc
      301 tttgtcactg tctaccttgg atttgttcat ctggagcatg agtacaagct catgggaatg
      361 gcgccctacg ggcgcgatcg tgacgcagaa cggatcgcag ataaactcga agggcttttt
      421 acttacacag atgcacgctg ggaattgaaa actccagtgt ggaatctcaa ttcagatgag
      481 cgcctggttt acgagtcttt gcgtgagatg ttcgaatttg aacgctttga caatatctgc
      541 gctggtctgc agcttttctc tgagagattc attgtccggt tcattcagga ttggattcgc
      601 aaaacaggtg ttaggcgagt cgctgtttct ggcgggttct tcatgaacgt taaggcaaat
      661 cagaaactgc gttccctgcc tgaaattgaa cagctctttg tctatccttc ctgtggtgat
      721 gagaccaatg caatgggcgc cgcttatttg aagtactttc aactcaccgg gaaaaggccg
      781 gagcctctga agaacttcac gctgggtcct gtaatcgatg attcgactct cctggatgtg
      841 ctaaaatcgt atggggaaaa aattcgtttt gaatattgcg aagatatcga ggagcgagcg
      901 gcggacctgc tatcgaaaaa gaacattgtg gctcgtgtca aaggccctga agaatttggc
      961 gctcgcgctc ttggcaatcg ttctattctg gcgcatccct ctgacactct tgcagtgcga
     1021 accatcaatg acatgattaa gtccagagat ttctggatgc ctttcgcctg cagtattctt
     1081 gaagaggaaa agctgcgtta cttgaaagat tggaccgcca acttttcccc caactacatg
     1141 accatgacgt atgactccaa aaattcagag gagatcctcg caggtatgca tcctcgagat
     1201 cgcactgtgc gtcctcaagt ggtgactcgc gaacagaatg ctgattacca tagacttctg
     1261 tcccgttttc atgacatgac agggatcggt ggcgttctca atacttcgta caatctccac
     1321 gggtttcctc tcgtgcatga cgcgagcgcc gccattgagg tcttcttgaa ttctggcctt
     1381 gagtacctcg cgctgaatca gtacctggtg accaaacctg gctagattat gataagtcgt
     1441 ttaaaacaat tcattggtgg attgcttcac cggtatagac ttggccagga agcacgacgg
     1501 catcgaaaag aagtcgaagg caaatcagtg gacgttgtgt tcactgagat attcaaacgc
     1561 aacttctggg gtgatcggga gagcgtctct ggaagcggaa gcaatcagga acaaacacgc
     1621 gtcatcgttc gcgaaattcc gagggtgctc aaacaggttg gcgctcgcac attgctggat
     1681 cttccctgtg gggattttaa ctggatgcag agagtagatc tatccggtgt ttcctatact
     1741 ggcggagaga ttgttcagga tcttgtggat ctcaatcaga agaagtttgg acgtgatggg
     1801 atttcattta agaagatgaa cctgattgac ggggatcttc cggcgagtga cgttcttcta
     1861 tgtcgcgact gccttgtgca tctttccaat gaacaaattc acgcgagtct tgcaaatatc
     1921 aagaagcacg gcatccgtta tctgctcact acatcgttca cggaaaccaa aaagaatagc
     1981 aacattgtca ccggagaatg gagaccgatc aatcttgaga tcccaccttt ttccctcaaa
     2041 gctgtggaag tcatcaacga agagtgcaca gaaggaaacg gccaattcgc agacaaaagt
     2101 ctgatcctag ttcgcatgaa ctagcccgga ggacggtatg attcaatcca aaaaaaatcc
     2161 acgcattcct acaaagaatc acgacggaaa acagaacggt tttttgattc caatcatcaa
     2221 tgtgaacgaa ggttttcctt cgccggatgc atggcctaaa caagtgtact gcacagtcgc
     2281 ctatcccgga caggtcaaag gcccacattt acacaagaag cgcgccggtc tttttacgtg
     2341 catcaaggga aatgttaaga tcgtcattcg aatcgatggg aaatatgtag aacacttcag
     2401 cggcgacgat catgaatatg caaccatcca ggtcccggcg ggaattccat ccgcgctcgt
     2461 gaatattggc aaagaagacg cttacatttt gaatatgcct tctcccgcct ggagcaaaga
     2521 cgagccagac gactgggatg tttcgtttga aggttacaat tttaacgcat gaatcgcacg
     2581 cttctgatta cgggtggagc cggtttcatc ggttcgcatt tgatcctgag gctcttcgag
     2641 cgctaccctg attatcgctt cgttaatatt gacaagctga cgtacgcctc agacgaatct
     2701 tatctcgatc caattcgttc gtccggtcgc tacacattcg cgaaagtcga tctggtgaat
     2761 aaggcggatg taactgcggt tttcgaagaa cataagccag acgggatcat tcatctggca
     2821 gcagaaacac atgt
//
LOCUS       DAOQJL010000511         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24420_length_2829_cov_4.041817, whole genome shotgun sequence.
ACCESSION   DAOQJL010000511 DAOQJL010000000
VERSION     DAOQJL010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24420_length_2829_cov_4.041817"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLG78_17135"
     CDS             complement(<1..949)
                     /locus_tag="PLG78_17135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE24900.1"
                     /translation="MNSPILALRIFWDRLGKRLAQKKRQGWSGITILILRDTAEEPVR
                     VRINQYVVYFAMLMVVALPLASAGVTILRHVKEERAFEKLEDRKSLLMNVPMILAERK
                     QLLRSARTQVFAFQSQLDNRDDKLFATWMDSLTEEPDRRSPEGIVGLDIEDLQRVRRS
                     AENTLSDGAYHAIRLFWHRISIYHLTPRGRPLLTGIGFISSGFGQRPDPFGNPTGETH
                     TGMDFAAAPKSPIVATSPGVVMQVSKSDRPGYGLFVRIHHGLGYTTLYAHCDSLLVNV
                     GDRVQRGQPIALLGRTGRATGHHVHYEVRFGDDKAIDPLE"
     gene            1035..>2829
                     /gene="pcnB"
                     /locus_tag="PLG78_17140"
     CDS             1035..>2829
                     /gene="pcnB"
                     /locus_tag="PLG78_17140"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNE24901.1"
                     /translation="MLRRLLSLFRGRPSADQVIPYPDGKRIYREFHSLRKEQMDPDAL
                     KVINRLSRHGYRSYLVGGCVRDMVLGKKPKDFDVVTQATPSQIRRIFANSRTIGRRFK
                     IVHVVFRGGKIVEVSTFRGLPGHRLEGKRKNQDLLMKKDNVFGTPKEDAARRDFTINA
                     LYFDPRNESIIDYVGGFDDIQARNLRVIGDPEVSFEEDPVRMFRAAKFAALLNFNLEA
                     ESLKGIRKKKDEMLKASSARMLEEYYKIFRTGKTFEIFRSLAETGLLSVLFPFLGKVD
                     GEEFRVSPAGRRLDIADKILTEREDLTTVIYLALLLAELVPDIFHGGTKTNIAEYVKN
                     KISAECTRLNIPGKERDRLTQIFVSQPRFQQSERGRGNRPDVFRSKIFFYEAFIIFKI
                     HALSRGDDDAIQQAMFWEFGPKIHPPSDSTLITMFPRRHRDRFGRPDRNDRPRDRDRG
                     RDRNRDMEPIRDDGIMSGKVRPERPRPERPERDRNEDRQDRPERPDRNRNEERVDRDR
                     NDRFDRNRTDKPESTDESGARSTDRADRPHDPDAVATGDQPPRRRRRRFRRRRGPRNG
                     NGGGNVTAENAENGDSGNPGSGNSPTTESGES"
BASE COUNT          713 a          733 c          754 g          629 t
ORIGIN      
        1 attcgagtgg atcgattgct ttgtcgtcgc cgaatctcac ctcatagtgc acgtgatgcc
       61 ctgtagctcg gccggttctt ccgagaagtg cgatgggttg ccctctctgg acacggtctc
      121 ctacattcac tagaagagaa tcgcagtgcg cgtagagagt cgtgtatcca aggccgtggt
      181 ggatgcgaac aaacaggcca tagcccggtc tgtctgattt ggatacctgc atcacaacgc
      241 cgggtgacgt tgcaacgata ggcgactttg gtgcggccgc aaagtccatt cccgtatgtg
      301 tttcacccgt gggatttccg aatggatccg gccgctgtcc aaatccagag ctgataaaac
      361 caattcccgt aagaagcggt ctgccgcgtg gcgtcaggtg ataaatggaa atgcgatgcc
      421 agaacaatcg gatcgcgtga taggcgccgt cagaaagagt attttcagca gagcgacgta
      481 ctctctgcag gtcttctata tccagcccca caattccttc aggagatctg cggtccggtt
      541 cttccgtcag cgagtccatc caggttgcaa aaagtttatc atcgcggtta tcgagctggg
      601 attgaaatgc aaacacctga gtccgtgcag atctgagcag ttgtttgcgc tcggcgagga
      661 tcatgggcac gttcatgagg aggctttttc gatcctccag tttttcaaag gccctttctt
      721 ctttcacatg gcgcagaatc gtaacaccgg cacttgcaag tggaagtgcc accaccatga
      781 gcattgcaaa atataccacg tattgattga tgcggactcg aaccggttct tccgctgtgt
      841 cacggaggat gagaatcgta attccgctcc agccctggcg cttcttctgg gccagacgtt
      901 tgcccagccg atcccagaag atgcgcaatg ccaggatggg cgaattcacg ctgatagcgc
      961 cgcacgggcc tctgtcatgg gaagcgaatt ttcacttgct ttcggggtcc aggcggggga
     1021 cccttacaaa acacatgtta cgtcgccttc tcagcctctt ccgaggccgc ccatccgccg
     1081 atcaagtcat tccctacccg gatggaaaac gaatctacag ggaattccac tcacttcgca
     1141 aagaacagat ggatccggac gctctcaaag tcatcaatcg cctcagccgc cacggatatc
     1201 gctcctatct ggtcggaggc tgtgtcagag acatggtcct gggcaaaaag ccaaaagact
     1261 ttgatgtagt gactcaggcc actccttcgc aaatccgtcg catcttcgca aactccagaa
     1321 ccattggcag acgattcaaa atcgttcatg ttgtgtttcg cggcggcaag attgttgaag
     1381 tctcaacatt tcgcggattg ccaggccaca ggctcgaagg aaaaagaaag aaccaggacc
     1441 ttctgatgaa gaaggacaat gtgtttggca ctccaaaaga agatgcagca cgtcgtgact
     1501 tcacgatcaa cgcgctctac tttgatccaa gaaacgaaag catcattgat tatgtgggcg
     1561 ggtttgatga catccaggct cgcaatctgc gtgtcattgg tgatccagaa gtttcttttg
     1621 aagaagatcc agttcgcatg tttcgcgcag ccaagttcgc ggctctcttg aatttcaatc
     1681 tggaagcaga atcgctcaaa ggcatccgca agaagaaaga tgaaatgctc aaggcatctt
     1741 ccgcgcggat gctcgaagag tactacaaga tctttcgcac aggaaagaca ttcgaaatat
     1801 ttcgcagcct ggctgaaacc ggactgctct ctgttctgtt cccgtttctt gggaaggtgg
     1861 atggagaaga gtttcgcgta agtcctgcag gcagaaggct cgacatcgca gacaagattc
     1921 tgacagagcg cgaagacctc accactgtga tttatctggc tctcttgctt gctgagctgg
     1981 tcccggatat ctttcacggc ggaacaaaga ccaacattgc agaatatgtg aaaaacaaaa
     2041 tttcagcgga gtgcactcgt ctcaacattc ctggaaagga acgtgatcgc ctgacacaaa
     2101 tctttgttag ccagccacgt tttcaacaga gcgaacgcgg ccggggcaac agaccggatg
     2161 tttttcgtag caagattttc ttttatgaag cgttcatcat cttcaagatc catgcacttt
     2221 ctcgcggaga cgatgatgcc atccagcaag caatgttctg ggagtttggt cccaagattc
     2281 atccgccttc tgacagcact ctgatcacaa tgtttcctcg caggcatcgt gatcgatttg
     2341 gtcggccgga tcgaaacgat cgtcctcgtg accgtgatcg cggaagagac agaaacaggg
     2401 atatggaacc cattcgtgat gatggcatca tgagtggaaa ggttcggcct gagcgtccgc
     2461 gtcctgaaag accggagcgc gataggaacg aagacagaca ggatagacca gaaagaccgg
     2521 atcgcaacag aaatgaagaa agggtggatc gcgaccggaa cgatcgcttt gaccgcaaca
     2581 gaacggataa accagaatca acagatgagt ccggggcacg ttctacagat cgcgcggacc
     2641 gtccccacga cccggatgca gtggctacgg gcgatcagcc gccgcgcagg cgcaggcgca
     2701 ggtttcgcag acgtcgcggc ccccgcaatg ggaacggagg cggcaacgtc acggctgaga
     2761 atgcagagaa tggcgattca ggcaatcctg ggagtggaaa ttcacccacc actgaaagcg
     2821 gagaaagct
//
LOCUS       DAOQJL010000512         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24527_length_2822_cov_3.272136, whole genome shotgun sequence.
ACCESSION   DAOQJL010000512 DAOQJL010000000
VERSION     DAOQJL010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24527_length_2822_cov_3.272136"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLG78_17145"
     CDS             complement(<1..451)
                     /locus_tag="PLG78_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNE24902.1"
                     /translation="MKTLRVSTATKKTRSKIAHEILSSGIHGPLLVVAGEHSGDLLGG
                     DVLSHLKKLGLKEFFGTGGETMKREGAEIIQDVESMAVMGFVDALRNYSRLHKLARDL
                     VARAKEKGARYALLIDYPGFNLRIAKMLHAEGIHVIFLVSPQLWAWHY"
     gene            complement(448..1305)
                     /gene="lpxI"
                     /locus_tag="PLG78_17150"
     CDS             complement(448..1305)
                     /gene="lpxI"
                     /locus_tag="PLG78_17150"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNE24903.1"
                     /translation="MKRLGILAGRGELPWIAARAAVAAGEDVRVFCFTNEEPPAEFKS
                     ISIKVVLTKLYSSVFKNMKAHNVGKVIMIGKATRDILYDNPRFDLRALLLLARMTNQA
                     DTTIFDFASREFEKRGFHIIPQDTHLKHLYLPEGRYGPALKAREIDDISFGMIYAREI
                     NRLDLGQTVVAGQLSILAVECAEGTDQCIRRGGGLFRNKGAVVCKVAKTAHDPRFDLP
                     VTGLSTLQSMKESGCRILAIESGRTLVVHHAEFIRTAQKMGISIVSCNPDKSDPKTLT
                     KLCKKAVRV"
     gene            complement(1422..1631)
                     /locus_tag="PLG78_17155"
                     /pseudo
     CDS             complement(1422..1631)
                     /locus_tag="PLG78_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(1703..2587)
                     /locus_tag="PLG78_17160"
     CDS             complement(1703..2587)
                     /locus_tag="PLG78_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNE24904.1"
                     /translation="MVRDYIKQLRVYQWTKSGFVVMPFVFSDYLPVVVSNPFSAGALE
                     LLFKLAAAFFAFSTLASSVYVFNDYRDRHQDALDSRKKHRPIASGRIGPAGAIAAIVI
                     LVGTSATLASFLPITFRIILSIYLIQNIFYTWLGKRILLIDVFMISLGFVFRVLAGAA
                     ALGIAASPWLLSCTLFLSLFLGFFKRYYELRTSPPEIMIGGQYTAESLKNFLSITASL
                     SVMNYAVYTLEGRYAGEKLYWTIPLVVLGIFRYYTLMDRPELEDGNPSDVLLTDKFLI
                     AVIGCWVLSCAALILHIA"
     gene            complement(2623..>2822)
                     /locus_tag="PLG78_17165"
     CDS             complement(2623..>2822)
                     /locus_tag="PLG78_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24905.1"
                     /translation="QIRLLASMGREISNVHCMRVESGLSAADSISSHLKSAPDRWLYH
                     AACTMADRIASRQKTWRRKMK"
BASE COUNT          667 a          719 c          758 g          678 t
ORIGIN      
        1 tgtagtgcca ggcccagagc tgtggtgaaa ccaggaaaat gacgtgaatc ccttctgcgt
       61 ggagcatctt cgcgattctt agattgaatc caggataatc gatcaagagc gcataacggg
      121 cacctttttc ttttgcgcgt gctacaaggt ctcgcgccag tttgtgcagt ctggagtaat
      181 tgcgaagtgc atccacgaat cccatgaccg ccatggactc tacatcctgg atgatctctg
      241 cgccttcgcg cttcatggtt tccccgccag ttccaaagaa ttctttgaga cccagtttct
      301 tcaaatgcga aagaacgtcc ccgcccagaa gatcaccgga atgttctcct gcgactacga
      361 gcagagggcc gtgaattcca gaggacagta tttcatgggc gattttgctt ctggtcttct
      421 ttgttgctgt ggacacgcgg agagtcttca tactctgact gccttcttgc acaattttgt
      481 cagagtcttt ggatctgatt tatccgggtt gcacgacaca atgctgattc ccattttctg
      541 cgcggtgcga atgaactcgg cgtggtggac tacaagagtg cgtccggatt caatggcaag
      601 gattctacat cctgattcct tcatggactg aagggtggaa aggcccgtga ctggaagatc
      661 gaaccgtgga tcatgggccg tctttgccac tttgcagacg actgcaccct tgtttctaaa
      721 tagaccgccg ccgcgacgaa tgcattgatc cgttccttcc gcgcattcga ccgcaaggat
      781 ggagagttgc ccggccacca ctgtctgtcc caggtccagt cggttgatct cacgagcgta
      841 gatcatgcca aaagaaatgt cgtcaatttc gcgcgccttg agggcgggac catatctgcc
      901 ttccggaaga tacagatgct tgaggtgagt atcctgagga ataatatgaa atccacgttt
      961 ttcaaattcg cgcgaggcaa aatcaaagat cgttgtgtct gcctggttgg tcatgcgtgc
     1021 cagaagcaac aatgctcgca ggtcaaatcg cggattgtcg tatagaatgt cgcgcgtggc
     1081 cttgccgatc atgatcacct tgccgacgtt atgcgctttc atgttcttga aaacggatga
     1141 atagagttta gtaagaacta ctttgatgga aatggactta aattctgcag gtggttcttc
     1201 gtttgtgaag cagaacacgc gcacatcttc gcctgccgcc accgcggccc tggccgcaat
     1261 ccatggcagc tcgccgcgtc cggcaagaat gccgagtctt ttcatgtacc cgaaccgctc
     1321 gaacctgaac cggtggaacc agtctggctg gagcttgcgg aagaacctcc ggacgaatcg
     1381 ctcgatccgg cgggcttact ttctccgccg cttttctcta cagtctttgg cgcgctcgtg
     1441 ctgctggacg ttttcttata gtccgttacg taaaaccctg accccttgaa aacgattccg
     1501 gctccggcac caatgagtcg ttccactctt ccgccgcatt tggggcagac tgtatctggg
     1561 tcatccttca tggactgaaa tgcgtcgtat tcatgaccgc aatcctggca ctgataggag
     1621 tatgtaggca tgctccaggt ttcgtttcaa tggccgtccg atcaagcagt tttgggctgt
     1681 cagtcggggg cagttccagg cgtcaggcga tgtgcaatat gagagccgcg caggatagta
     1741 cccagcagcc aatcactgcg atgaggaatt tatctgtcaa aagtacgtca gagggattcc
     1801 cgtcttccag ctccgggcga tccatcaaag tgtagtagcg aaagattcca agcactacga
     1861 gtggaatcgt ccagtagagt ttctcaccgg catacctgcc ttccagggtg tagactgcat
     1921 agttcatcac gctaagagaa gccgtgatgc ttagaaagtt cttcaggctt tccgcagtgt
     1981 actgcccacc gatcatgatc tccggaggag atgtgcgaag ttcgtaatat ctcttaaaga
     2041 aaccaaggaa gagagacaaa aacaaagtgc acgaaagtag ccaggggctg gcggctattc
     2101 cgagcgctgc ggcaccggcc agcacgcgaa aaacaaaccc gagagaaatc atgaacacat
     2161 ctatgagcag gattcttttc ccaagccagg tatagaaaat attctgaatg agataaatag
     2221 agagaatgat tctgaaagtg atggggagaa acgatgcgag agtggcggag gtgcctacga
     2281 ggatgacgat ggcggcgatg gctcctgccg gcccaatgcg gccgcttgca atgggtctgt
     2341 gtttcttgcg actgtccagg gcatcctggt gcctgtctct gtaatcattg aatacataca
     2401 cagaacttgc gagagtagag aatgcaaaga atgccgctgc cagtttgaac agcagctcca
     2461 gggcccctgc gctaaaagga ttgctgacta cgactggaag gtagtcagaa aaaacaaacg
     2521 gcatcacaac aaagccggac ttggtccact ggtagacgcg cagctgcttg atgtagtccc
     2581 ggaccatacg cgatcgtccg agcctatcgt cgcggggtca actcacttca tttttctgcg
     2641 ccaggttttt tgtctcgagg cgatgcggtc ggccatggtg catgcagcat ggtagagcca
     2701 ccgatcagga gcacttttta gatggctgga gatcgagtcc gccgcagaca ggccagactc
     2761 tacgcgcata caatgcacgt ttgagatttc gcggcccatg gacgcaagga ggcggatctg
     2821 at
//
LOCUS       DAOQJL010000513         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24562_length_2819_cov_4.249638, whole genome shotgun sequence.
ACCESSION   DAOQJL010000513 DAOQJL010000000
VERSION     DAOQJL010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24562_length_2819_cov_4.249638"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="plsX"
                     /locus_tag="PLG78_17170"
     CDS             <1..698
                     /gene="plsX"
                     /locus_tag="PLG78_17170"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNE24906.1"
                     /translation="TGATMAAALLEMGRIKGVERPSIASPLPREDGGTTVLIDSGANV
                     DCKASWLVQFAIMGEIYSREILGVVNPRVAVLSNGEEEGKGNSVSTDAWERLRHLPCN
                     FMGNVEGRDLYGGGRTADVVVCDGFTGNIVLKATEGLATSIFTILLQGIAGSSLAKTG
                     AYLLKPVLSEVKRRMDYTEYGGAPLLGVEGICIIGHGGSNATAFKNAIRVVDQSSKRD
                     IRQKIEDRVRLFG"
     gene            complement(734..1069)
                     /locus_tag="PLG78_17175"
     CDS             complement(734..1069)
                     /locus_tag="PLG78_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24907.1"
                     /translation="MRKPILLSIMFLLILSCKGQPVPENKKSYVGTWVGDGITLIISS
                     DGGLSYQKVRSAGRTSVHGPIQEFAEREIRAGVWIFSAKFRIDQEPALVDGKWMMEVD
                     GEKLTKLGP"
     gene            complement(1235..>2819)
                     /locus_tag="PLG78_17180"
     CDS             complement(1235..>2819)
                     /locus_tag="PLG78_17180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNE24908.1"
                     /translation="DSVTIYTDRATVRRVMPVGVDAGGVVRFSDLPAAVLPDSIRASA
                     SNLDIVSVAVQPRAKSYEDMSDHPLNAKIESLQSEIRMETDRQSNYREQLKVLSSFGQ
                     IASGQSDRELKQNVSVIAGWTDALRFLEKNRQEYLEKIQKSERRNVELKRDLEKTSGE
                     LARILAARKKSQVEVVIQYAGTPRAGSSVSLEYTVTGVSWQAVYDLHGSSEGGDFRLD
                     ARAAVRQATGEDWNNVRITLSTARPSVALAPGQLRPWRISGKDLWTMDRDEGKRDDSA
                     GETESSAGDSQDGASFSIQLPARETIESDNSDHRITLQSTPLKGTVNHVAIPSMTDSV
                     FLRAKFRNSSSIPLLTGAMNIFLDGSFAGTTTTPRAAAGEDFDLYLGADQRMTARRVL
                     LKGEVAGAGILSSKVQIQNQWQIEIANHSRKPRSVIVFDRYPVARDPNISTKFQGSNR
                     SDVKPDANGMLAVNMNVDPGASQKFDFSYSLEIPQETWQKFQENLENEKGRRPAQPAV
                     EPNAPAKQRAYDLERMLMH"
BASE COUNT          680 a          739 c          722 g          678 t
ORIGIN      
        1 atactggtgc gacaatggcg gcggctctcc ttgaaatggg gcgaatcaaa ggagtggaga
       61 gaccatcgat cgcgagccct ctgccgcgcg aagacggggg aaccacagtg ctcattgatt
      121 caggcgcaaa cgtagactgc aaggcaagct ggctggttca atttgcgatc atgggagaaa
      181 tctattcacg cgaaattctt ggtgtcgtta acccgcgcgt cgccgttctc tccaatggag
      241 aagaagaagg aaaaggaaat tctgtttcaa cagatgcatg ggagagactg agacatttgc
      301 cgtgcaactt tatgggcaat gtggaaggaa gagatctata cggaggcgga cgaactgcgg
      361 atgtcgtcgt gtgcgacgga ttcacaggca acattgtgct caaagcaacc gaaggtctgg
      421 ccacatcgat atttacgatt ctattgcagg gcatcgctgg atcaagtctt gcgaagacgg
      481 gagcgtattt gctcaaacct gtcctttcag aagtgaaacg aagaatggat tacaccgagt
      541 acgggggagc gcctttgctc ggggtagaag gaatctgcat cataggtcac ggtggttcca
      601 acgcaactgc attcaaaaac gcaattcggg tggtggatca atcctccaaa agagacatcc
      661 gccagaagat agaagacaga gtgcgtcttt tcgggtgaag cccgtaagga aacgggggcc
      721 tgccaggcaa gattcagggc ccaagcttcg tcagtttctc tccgtccact tccatcatcc
      781 atttcccgtc tacaagtgct ggctcctgat cgattcggaa cttcgcagaa aagatccaga
      841 caccggcacg aatctcccgc tctgcaaact cttgaatggg accatggacc gatgtgcgcc
      901 cggcgctccg caccttctga tagctgagcc caccgtcact gctgatgatc agggtaatcc
      961 cgtcacccac ccacgtgcct acgtaactct tcttgttttc cggaactggc tgtcctttgc
     1021 acgaaagaat cagcaaaaac ataatcgaaa gtagaattgg tttcctcatg actgatattg
     1081 agacataggc gcgtatgttt gcaagcattg tctgcaaagg cgaacttccg atccgcatcg
     1141 cgttatcacg cataaaatgt tcgttatact taccaacgcc cggtgttatc acgcactttt
     1201 gttgcgtgat aaagcacacg cacgccagag agcttcaatg catcagcatt ctctcgagat
     1261 cgtatgcgcg ttgtttggcg ggagcattcg gctctaccgc tggctgggcg gggcgccttc
     1321 ctttttcgtt ctccagattt tcctgaaact tctgccacgt ttcctgggga atctccagcg
     1381 aataagagaa gtcaaatttt tgagaggcgc ccggatccac gttcatattc acagccagca
     1441 tgccgtttgc atccggcttc acgtcgctcc ggttcgaccc ctgaaacttc gtggagatat
     1501 tggggtctcg cgccacagga tagcgatcaa acacgatgac agacctgggc tttcgactat
     1561 gatttgcgat ttcgatttgc cactggtttt ggatctgcac tttgctcgat agtatgcccg
     1621 cgccagccac ttctcccttg agcaaaacgc gacgcgcggt catgcgctgg tctgcaccca
     1681 gatacaaatc gaagtcctcg cctgcggcgg cacgaggtgt agtagtcgtt ccggcaaaac
     1741 ttccgtccag aaagatgttc atggcaccag tcaaaagagg aatggaagaa gaattacgaa
     1801 acttcgcacg aagaaataca gaatcagtca tagagggaat cgccacatga ttgacagtgc
     1861 ctttcagagg agtgctctgg agggtgattc gatgatctga gttgtccgat tcaatggttt
     1921 cgcgagcagg gagttggata gaaaaagaag ctccatcctg ggaatcaccg gcactcgact
     1981 ctgtttctcc ggctgaatcg tcgcgctttc cttcatcgcg atccatggtc cacagatctt
     2041 ttccagaaat tctccatgga cgaagctgtc ccggagcaag agcgactgac ggtcttgcgg
     2101 tggataacgt tatgcgcaca ttattccaat cttctcctgt ggcctggcga acagcggccc
     2161 gggcgtccaa cctgaaatcg cctccttccg aggatccgtg caaatcatac actgcctgcc
     2221 aggaaactcc cgtaactgtg tactcgagcg aaacactcga tccggccctc ggagtgcctg
     2281 catactgaat cacaacttcc acctgagatt tttttcgcgc ggcaagaatt cgcgccagct
     2341 cgccacttgt cttttcgaga tcgcgcttga gttctacatt tcttctttct gacttttgaa
     2401 tcttctccag atattcctgt ctgttctttt caagaaaacg aagcgcgtcc gtccagcctg
     2461 caatcacgct cacattctgc tttagttctc tgtctgattg accggatgca atctgtccga
     2521 aggaagaaag gaccttcaat tgctcacgat agttggattg cctgtccgtt tccattcgaa
     2581 tctcagactg cagcgattcg atcttcgcgt tcaaggggtg atcggacatg tcctcgtaac
     2641 tttttgcacg cggctgaact gcgacggata cgatgtcaag attggaggcg gacgctctga
     2701 tggaatcagg taaaacagcg gcaggaagat ccgaaaagcg aaccacgcca ccggcatcta
     2761 cgcccactgg catgacgcga cgcacggttg ctcgatctgt atagatcgta acagagtcc
//
LOCUS       DAOQJL010000514         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24564_length_2819_cov_3.875904, whole genome shotgun sequence.
ACCESSION   DAOQJL010000514 DAOQJL010000000
VERSION     DAOQJL010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24564_length_2819_cov_3.875904"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLG78_17185"
     CDS             <1..630
                     /locus_tag="PLG78_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE24909.1"
                     /translation="DLTQRLILQSNAQTLYGQSVARVEKMDSGFRITTAQTTVDADCL
                     FYCAGTGTPQFVDAPVSATRGQIHYIPPWNETLRTVICADGYLIPGNDFWTAGSTYSR
                     TDTTSSPHIEGQNEILEKLRNAIPDVPHFETLLDGRVGYRISAKDRFAIFGQLGDHAF
                     LSTAHGSRGIVSAPLAAEVIASLACGMPVPVESDLMNHFQNRYAIKHKL"
     gene            complement(625..2658)
                     /locus_tag="PLG78_17190"
     CDS             complement(625..2658)
                     /locus_tag="PLG78_17190"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE24910.1"
                     /translation="MRTLIACILLALASCSVRTGEAEQGVLDLRSWDSQSDVISLDGE
                     WLFAPGLSPRPADFQVVRLPADSDFHSQKIETGCVTLKLKLRLPESVNKVGLISGKQL
                     SSHRILWNERVVAGAGTPACSAPEYEPSLEPSVSFWNVKGGDSEIVLQIANFDNYEIS
                     AGRILLGSEKTAQTFLLRRVAGEIVTLGALIIVAFYHLGLFALRRRELSHLMVGVLCI
                     TGLAHFASSGIHLVPLYLNLPYSMYVRTDYISWFFAGSVFVHYTVLALRLTPPVWIVP
                     ANYATSVALTLFTLFTKPILFTQITPFFEVAFAVQCAYSLYLLLDGMRRDREGARLLF
                     AGAVILFAAIVNDGLNEKDIIHTQAFAPAGLLVFLAFEGLVTARGFASAFGAVEDLSV
                     RLIDTNQAYTRFMPAEFLGYMEKESITDVRLGDHIQKEMTVVFADIRDFTAMSEKMTP
                     AETFEFLNGYLSRVGHIIRDHEGWIDKYIGDGIMALFPRSADDAVKSAIEMQHEILRY
                     NKERATKGRSPIHVGIGIHTGTLIMGIIGENERMEGTVISDVVNVASRIEHLTKVYAS
                     SILISGETLVNLEDSLAYHFRLLGRVPVKGKSRPVSVVEILDGRPELEVMLATKVEFE
                     KGFNALMIGHDQDAIRCFEYVLSQNPADGAAALYLSRLRGTEDPSKAIFIPSL"
BASE COUNT          742 a          743 c          693 g          641 t
ORIGIN      
        1 gacttaactc agagattgat cctacaatcc aatgcacaga cgttatacgg ccaatcggta
       61 gctcgcgtag aaaaaatgga ttccggtttt cgcatcacaa ccgcacaaac cacagtcgac
      121 gcagactgcc tcttctactg cgccggaacg ggcaccccgc aattcgtaga cgcaccggta
      181 agcgccaccc gcggacaaat acactacatt ccgccctgga acgaaactct acgaacagtg
      241 atttgcgcgg acggatactt gattcctggt aacgatttct ggactgccgg gtctacctac
      301 agcaggacag acaccacatc atcgcctcac atagaaggcc agaatgaaat cctcgagaaa
      361 ttgcgaaacg ccatcccgga tgtgcctcat tttgaaactc tactggatgg aagagttgga
      421 tatcgcatat ctgcgaaaga cagatttgcc atcttcgggc agttaggcga tcatgcgttt
      481 ctatccaccg ctcacggctc caggggaatt gtaagtgcac cacttgcggc cgaggtgatt
      541 gcatctcttg cctgcggaat gccggttcct gtagagtcgg atctgatgaa tcactttcag
      601 aaccgttatg cgatcaaaca caagctatag acttgggatg aagattgctt tggaaggatc
      661 ctcagtaccg cgaaggcgag acagatagag cgcggccgca ccgtctgcgg gattctggga
      721 aagaacgtat tcaaagcagc gaatcgcatc ctgatcatgt ccaatcatga gtgcgttgaa
      781 gcctttttca aattcgactt tggttgcaag catcacctca agctccggtc ggccgtcgag
      841 gatttccacc acggataccg gtctgctttt tcccttcact ggaactcgac cgaggaggcg
      901 aaagtgatac gcaagagaat cttccaaatt tacgagggtt tctccgctga ttagaatcga
      961 agatgcatac accttggtta aatgctctat gcgagatgca acgttgacca catcagagat
     1021 cacagtgcct tccattcttt cgttttcccc aatgattccc atgatcaagg tgcctgtgtg
     1081 gatgccaatt cccacatgaa tgggggacct gcccttggtc gctcgttcct tgttgtagcg
     1141 aagaatttca tgttgcattt caatggcgct tttgaccgcg tcgtctgcgc tccttggaaa
     1201 tagagccatg atgccgtcgc cgatgtactt atcaatccag ccctcatggt cacgaatgat
     1261 gtgtcccaca cgcgaaaggt atccgttcaa gaattcaaac gtttctgctg gtgtcatttt
     1321 ctccgacatc gcagtgaaat ctcgaatatc cgcaaagaca acggtcatct ccttctgaat
     1381 atgatctccc agacgaacat ccgtgatgct ttccttctcc atgtaaccca gaaactccgc
     1441 aggcatgaac ctggtgtatg cctggtttgt atctatgaga cgaacggaca aatcttccac
     1501 tgccccaaac gcactggcaa agccgcgcgc cgtgacaagg ccttcgaacg cgagaaacac
     1561 aaggagcccg gcaggagcaa aagcctgcgt atgaatgatg tctttttcat tcagtccgtc
     1621 gtttacgatg gcagcaaaca gaatcaccgc acccgcaaac agaaggcgcg cgccttccct
     1681 gtcgcgccgc atcccgtcca aaagcagata aagcgagtag gcgcattgca ccgcgaaggc
     1741 gacttcaaag aatggagtga tctgggtgaa caggattggt tttgtaaaaa gagtaaagag
     1801 agtcagagca acggatgtgg catagtttgc cgggacaatc cacacaggag gagtcaggcg
     1861 caaagcgagc accgtgtagt gcacaaaaac agatcccgca aaaaaccacg atatatagtc
     1921 cgtgcgaacg tacatggagt atggcaggtt cagatagagt ggcacgagat gaattccgga
     1981 agatgcaaaa tgagcaagcc ctgtaataca aagaactcct accatcaaat gcgaaagctc
     2041 gcgtctcctg agtgcgaaaa gccccagatg gtagaacgca acgatgatca aagcgccgag
     2101 ggttacaatt tcccccgcca ctcttctcaa aagaaaagtt tgagctgtct tttcggaacc
     2161 aaggagaatt ctccccgctg agatttcgta gttatcaaaa ttcgcaatct gtaagacaat
     2221 ttcagaatct ccacctttca cattccagaa agatacggat ggttcgagag aaggctcata
     2281 ctcaggtgca gaacaggcgg gcgttcccgc accggccacg acgcgttcgt tccagaggat
     2341 gcgatgagag ctcaattgct tccctgaaat caaacccacc ttattcacgc tctcaggcag
     2401 tcgcagtttg agtttcagag tgacgcagcc ggtttcgatt ttttgagaat ggaaatcgct
     2461 gtccgcgggc agtcggacga cctggaagtc agccgggcgc ggagatagcc ccggggcgaa
     2521 taaccattcg ccgtccagac tgatcacgtc gctctgagaa tcccaggatc tgagatcgag
     2581 aacaccctgc tccgcttcac ccgtgcggac ggagcaagag gcaagagcca gaaggatgca
     2641 tgcgatcagc gtgcgcatgt tcgatgaacg caagaaacga ccacccgcgt cagccatttt
     2701 ccacccgggc gatgcatgat ttttgtctta caaaaatcag cgggaaattc tatttcttcc
     2761 ggcttgtctt tgacttcgta gctgtctttg acttcgtagc tgtctttgac ttcgtagct
//
LOCUS       DAOQJL010000515         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24815_length_2804_cov_4.189887, whole genome shotgun sequence.
ACCESSION   DAOQJL010000515 DAOQJL010000000
VERSION     DAOQJL010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24815_length_2804_cov_4.189887"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1862)
                     /locus_tag="PLG78_17195"
     CDS             complement(78..1862)
                     /locus_tag="PLG78_17195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNE24911.1"
                     /translation="MQELSEKPGLPAWDLKAIYPDSSSRDADVALLRSLVVKFVEEYR
                     GRLGSLDDDGLVRAFEAHAKISNTSSRIMVHAYLTFAENTASTAAQALLASAEELSAE
                     VWGQLVFFELELGRLPGQFSSRMDAYAYFVQKCVQRNAHNLTEELEAYGIEKNLTGVQ
                     AMTGLFDEVMGSLSFEDKNASGEDTTFTQETALSALHHADRAYRLRIYSRFLETVGSQ
                     KTVLTSIYNNVLLDHRLDWKRRNYTGAMEPRNLSNQVSSRAVDSMLEIVQSSYRIVRR
                     YYTWKARYAKIDQFTNADIYAPVLPRTSPYDFAHAQDSVIRAYAGFDPQAGSIVSEFF
                     KTARVDARVIPGKRPGAFCYGASPELDAFVHLNYTGDLRSIQTLAHELGHGLHHQLSR
                     SQNHVNFGTPLVTAETSSVFGEILLNELLIQEARSKEEQLALLCAQMEGILATVFRQT
                     VLTRFEERAHAERDSGRVSADRFSELWLEENGKLYGDAVSMVDAYKWGWAYIPHFVHT
                     PFYCYAYSFGQLLVLALYQEFRTHGDSFKSGYMKLLSLGGSRAPAKLVSETVGLNIES
                     DDFWLRAVSFIENMMDRIESIGPGPGAA"
     gene            1857..>2804
                     /locus_tag="PLG78_17200"
     CDS             1857..>2804
                     /locus_tag="PLG78_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24912.1"
                     /translation="MHGFKYAWTIRSPPEFTVCKSARILILARTSAMLFNSGIFILLF
                     FAVYSFYWLLPVRAKQYMIIITSILFYGWYSVPFLLMFLALLIINYYVGMSLIDKKNK
                     KVLIFGIVVDVMVLVIFKYFYLLAETVGHITGSAYLIDLRNSWVADYKFEIILPIGIS
                     FYTFQVIAFITDCYTGMIREKVTPMKFYVFTLFFPHFVAGPIMRAPDLIPQIDNPSID
                     RTRMLNACLLILMGIVKKVLIADRLGAATSAIWHNPEKYDAIFLLLIPFVWAFQIYGD
                     FSGYTDMARGIAKLLGFHIPENFRGPFVARSYQELWQRWH"
BASE COUNT          686 a          722 c          693 g          703 t
ORIGIN      
        1 tagtgatttg catgatctgg cattaccaaa tgacgagttt ctacggaaga agagcgcggc
       61 gttttagcag acagggctca cgcggctccc gggcccggcc caatcgattc aattctgtcc
      121 atcatgtttt cgataaaact cacagcgcga agccagaaat cgtctgattc aatgttcaga
      181 cccaccgttt cgcttacaag ttttgcaggc gctcttgatc ccccaagcga tagcaacttc
      241 atgtaaccgg acttaaacga atctccgtgc gtgcgaaatt cctgatacag tgcgagcacg
      301 aggagctgtc cgaatgaata ggcgtaacag taaaatggag tgtgaacaaa atgcggaata
      361 tacgcccatc cccatttgta tgcatccacc atggatactg cgtcgccata gagctttcca
      421 ttttcttcga gccacagttc gctgaatcga tccgcggaaa cacgtcctga atctctctct
      481 gcgtgagctc tttcttcaaa acgcgtaagc actgtttggc gaaatacagt ggccagaatt
      541 ccttccattt gcgcgcagag gagagccagt tgttcttcct ttgatcttgc ctcttgaatc
      601 agaagctcgt tgagcaagat ctctccgaac acagaggaag tctcagcggt cacaagaggg
      661 gttccgaaat tcacatggtt ttgactgcgt gacagctggt gatgcaatcc atgtcccagt
      721 tcatgcgcga gagtctgaat ggagcgaaga tcgccggtgt agtttagatg cacgaatgca
      781 tccagctcag gggaagcacc gtagcaaaaa gcacccggac gtttgcccgg aatgacgcgc
      841 gcatccactc tcgctgtctt aaaaaattca gaaacgatcg aacctgcctg aggatcgaat
      901 ccggcgtaag cacgaatcac tgaatcctgc gcatgagcaa aatcgtatgg actggttcgt
      961 ggaagaacgg gagcatagat gtccgcgttg gtaaactggt caatcttcgc ataacgtgct
     1021 ttccaggtgt agtatctcct gacgatgcga tagctggatt gaacaatctc gagcatcgaa
     1081 tcgactgcac gcgaactcac ctggttggat agattgcgag gctccatcgc accggtgtag
     1141 ttccgcctct tccaatctag cctgtgatcg agtaagacgt tgttataaat actggttagt
     1201 acagtcttct gcgaacctac agtctcgaga aatctactgt agatgcgaag gcgataggcg
     1261 cgatccgcat gatggagcgc agagagcgcg gtttcctggg tgaaggttgt atcctctccc
     1321 gacgcatttt tgtcttcgaa agaaagagat cccatcacct catcaaagag gccggtcatt
     1381 gcctgaactc cggtcagatt cttttcgatt ccgtaggctt cgagttcctc cgtaaggttg
     1441 tgcgcatttc tctgtacgca tttctgaaca aagtacgcgt acgcatccat gcgagaagag
     1501 aactggcccg gcaatcgccc cagctcgagc tcgaagaaca caagctggcc ccagacttca
     1561 gcactgagtt cctccgcact tgccagaaga gcttgcgcgg cagtgcttgc cgtgttctct
     1621 gcgaaggtca gatatgcatg caccatgatg cgcgaagatg tgttagaaat ctttgcatga
     1681 gcttcaaaag cccggaccag accatcatcg tccagagagc ccaggcggcc gcggtattct
     1741 tccacgaact tgacaacgag gctgcgcagc agtgcgacat ccgcgtcgcg agaagatgaa
     1801 tccggataga ttgcttttag atcccaggca gggaggccgg gcttttcaga gagttcttgc
     1861 acggcttcaa gtatgcctgg actatccgca gtccaccaga atttacagtt tgcaaatcag
     1921 cgcgtatcct gatccttgct cgaaccagcg ccatgctttt taactcagga atcttcatcc
     1981 tgctgttctt cgcagtgtat tcgttttact ggctcctgcc agtgcgtgcg aagcagtaca
     2041 tgatcatcat cacgagcatt ctgttttacg gctggtacag tgtgccgttt ctgctcatgt
     2101 tcctggcact tctcatcatc aactactatg ttgggatgag cctcatagac aagaagaaca
     2161 agaaagttct gatattcggg atcgtagtcg atgtgatggt cctggtaata tttaagtatt
     2221 tttacctttt ggctgaaacg gttgggcaca tcacaggcag cgcctatctg attgatctgc
     2281 gaaacagctg ggtcgcggac tataagtttg aaatcatcct tccaatcggg attagcttct
     2341 acacgtttca ggtgatcgcc ttcatcacag attgttacac aggaatgatc cgggaaaaag
     2401 taactcccat gaagttttac gtgttcactc tcttcttccc gcactttgta gccgggccca
     2461 ttatgagagc gccggatctc attcctcaaa tagacaaccc gagcattgac agaaccagaa
     2521 tgctgaacgc ctgcctcctg atcctgatgg gcattgtaaa aaaagtgctc atcgcagaca
     2581 gactgggcgc cgccacttct gcgatctggc acaatcctga aaaatacgat gcgatcttcc
     2641 tgctgctcat tccgttcgtc tgggccttcc agatttacgg ggatttctcc ggatatacgg
     2701 acatggcgcg aggaattgca aagctcctcg gatttcacat cccggagaac tttcgcggac
     2761 cattcgttgc cagaagttat caggagctct ggcagagatg gcac
//
LOCUS       DAOQJL010000516         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24845_length_2802_cov_4.353112, whole genome shotgun sequence.
ACCESSION   DAOQJL010000516 DAOQJL010000000
VERSION     DAOQJL010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24845_length_2802_cov_4.353112"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLG78_17205"
     CDS             <1..298
                     /locus_tag="PLG78_17205"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE24913.1"
                     /translation="EEIDTHPALFLGVEPDQGYKTTTVSVQPSDKLLLYTDGLTEPLN
                     DAGEQYGERVFGEFLRSRAGLEPSEILAGLAVSMEGFTTENLRDDVAIVCVKVE"
     gene            complement(301..1293)
                     /locus_tag="PLG78_17210"
     CDS             complement(301..1293)
                     /locus_tag="PLG78_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNE24914.1"
                     /translation="MLSRTADSIYWLNRYIERAENYARFLDVNMQLALDFAEGAQWMP
                     LVETTGDREQFLEAYGEANADNVIRFLISDEKNPNSILSCVSIARENARAVREMISTE
                     MWLHINRFYLTLTESAAPGIQVANYSEFLAWVKDNCALHAGLMDGSLSHTEAWHFGKL
                     GRSVERADKTSRLLDIKYFYLLPGAEQVGSEADLIQWQAVLRSASALEMFRREYNRLT
                     PANVAGFLILDRHFPRSIRFCITEGERSLRELTGGDPQAFRNEAEKRMGSFRSQLEYT
                     EVGDIFRHGLHEYLDLIELRLNEIGEAIQEVFLVPPDTSSMPAGAVFPQAQQQA"
     gene            complement(1287..2723)
                     /locus_tag="PLG78_17215"
     CDS             complement(1287..2723)
                     /locus_tag="PLG78_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE24915.1"
                     /translation="MDLGGYQLDGFYDEMFRPDGTLRPAYEGFSSAISGWSREELLRR
                     KRMAERSLLNRGITFQVYGDASRKEQIFPFDAIPRIIEAKEWKQMEKGLKQRTFALNE
                     FINDIYHDKKIVKDGVIPTWLLASAKSYLPVCEGLNPPRGIWCHINGVDLVRSGDGRY
                     YVLEDNLRCPSGVSYVLVNRENMKRTFPEIFLKQGVLPVYDYPYKLKKMLSYISGKED
                     PVLGVLTPGQYNSAYFEHGFLAQQMGAELVEGSDLIVYQHEVYMKTTRGLKRVDAVYR
                     RIDDTFMDPLAFRPDSMLGVPGIFEAYRRGNFAMANAPGTGIADDKAVYAYVPRMIKY
                     YTGEDPILPNVPTFVCADDKEKRHVIQNLENLVVKAANEAGGYGMLIGPRASKQEIEE
                     FRVRIHENPRNYIAQPVLDLSRVPVITDQGIEGRHVDLRPYIIYGEDIFVLPGGLTRV
                     ALKKGSLVVNSSQGGGSKDTWVLPDPPC"
BASE COUNT          708 a          765 c          650 g          679 t
ORIGIN      
        1 cgaggaaata gacactcacc ctgccctttt cctgggcgtt gaaccggatc agggctacaa
       61 aaccacaact gtgagcgttc aaccctctga caaactactt ctctatacgg atggacttac
      121 agaaccactg aacgatgcag gagaacaata cggagaaaga gttttcggag aattcttgag
      181 atcgcgggcg ggtcttgaac cgtcagaaat cctggccggt cttgctgtgt ccatggaagg
      241 attcaccact gaaaatctgc gagacgatgt tgccattgtc tgcgtcaagg tcgaataacg
      301 ttatgcttgc tgttgggcct gcggaaagac agcacctgcc ggcatagagc tcgtatccgg
      361 cggaaccaga aaaacttcct gaattgcctc cccgatttcg ttcagtcgca gctcaatcaa
      421 atccaggtac tcatgcaaac catgacgaaa tatatcgcct acttccgtat actccagctg
      481 ggatctaaaa gaccccattc ttttttccgc ttcattgcga aatgcctgag gatctcctcc
      541 tgtcaattca cgcaaagaac gttcaccctc tgtaatgcag aaacgaatgg atctgggaaa
      601 gtgtctgtcc agtatcaaga aaccagccac gttagccggc gttaaccgat tatattctct
      661 acgaaacatc tcaagagcac tggcgctacg aagcaccgcc tgccactgaa tcagatctgc
      721 ttcagagcct acctgttccg cgccaggaag aagatagaaa tacttaatgt ccagcaaacg
      781 cgaagtctta tctgcccttt cgacggatcg acccagcttt ccaaaatgcc atgcctccgt
      841 atgcgaaaga gagccatcca tcaagccagc atggagagca cagttgtcct tcacccacgc
      901 aagaaactcc gaataattag ccacctggat tccgggtgct gcgctctccg tcagagtcaa
      961 ataaaaccga ttgatatgga gccacatctc cgtagagatc atttcacgaa ctgcgcgggc
     1021 gttttctcgc gcgatgctca cgcacgatag aattgaatta ggattctttt catccgagat
     1081 caaaaagcga atcacgttgt ctgcattcgc ttctccataa gcctcaagga attgctcccg
     1141 atcacccgtg gtttccacca gaggcatcca ctgtgcgcct tcggcaaagt cgagggctag
     1201 ctgcatattc acatcgagaa acctggcgta gttttccgct cgctcgatgt aacgattcag
     1261 ccagtagatc gaatctgccg tgcgacttaa catggcgggt caggcaggac ccaggtgtcc
     1321 ttgcttcctc cgccctgcga agaattcacg accagcgaac cttttttcaa agccactcgc
     1381 gtgagtccgc cgggcaatac aaagatgtct tcgccataga tgatgtatgg acgtagatcg
     1441 acatgtcgtc cttcaattcc ctgatctgta ataacaggca cgcgcgacaa atcgagaaca
     1501 ggttgagcaa tgtagtttct tggattctca tggatccgca cgcggaattc ttctatctcc
     1561 tgtttgctcg cacgcggtcc gatcaacatt ccgtagccac cagcttcgtt ggctgccttt
     1621 actacgagat tctctagatt ctgaattacg tgtctcttct ccttatcgtc cgcgcaaaca
     1681 aacgtgggca cgttaggaag gatgggatct tcacccgtgt agtacttgat catgcgcggc
     1741 acatacgcat aaacagcttt gtcgtctgcg attcctgttc ctggagcgtt tgccatggca
     1801 aagttgccgc gacgatatgc ctcaaaaatt ccaggaacac ccagcattga atccgggcga
     1861 aacgcaagag gatccatgaa cgtgtcatca atcctacgat agacggcatc gactcgcttg
     1921 agcccgcgag tagtcttcat gtatacttcg tgttgatata caatcaggtc agaaccttct
     1981 accagttccg ctcccatctg ctgagccaga aagccatgtt cgaaatacgc agagttgtac
     2041 tgaccaggag tgagaacacc cagaaccggg tcctcctttc ctgagatgta ggaaagcatc
     2101 ttcttgagtt tgtacggata gtcataaacg ggaagcacac cttgcttgag aaaaatctcc
     2161 gggaaggtgc gcttcatatt ctcccggttt acaagcacat aggaaacgcc ggaaggacaa
     2221 cgcaggttgt cttcgagaac atagtagcgg ccgtccccgg agcgaaccag gtccactcca
     2281 ttgatgtgac accagattcc tcttggagga ttcaatcctt cgcataccgg aagatagctt
     2341 ttcgcggagg caagtagcca ggtcggaatg acaccatcct tcacaatctt cttgtcgtga
     2401 tagatatcgt tgataaactc attgagcgcg aaggtgcgct gcttgagtcc tttctccatc
     2461 tgcttccatt cttttgcttc gatgatgcgc gggattgcat caaaaggaaa gatctgctct
     2521 ttgcgcgaag cgtcgccgta gacttgaaac gtgattccgc gattcagcag ggatcgttct
     2581 gccattcgct ttcgcctgag caactcttcc cgactccagc ccgagatcgc tgagctgaac
     2641 ccttcataag cagggcgaag tgtcccgtcc ggtcggaaca tttcgtcata gaaaccgtcg
     2701 agctggtatc ccccgaggtc aatgcgactt tcgccgctat catctactgc aagcccaaat
     2761 gaaaccatac agccataatt gcaatgtccg taccatggct gt
//
LOCUS       DAOQJL010000517         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24846_length_2802_cov_3.801966, whole genome shotgun sequence.
ACCESSION   DAOQJL010000517 DAOQJL010000000
VERSION     DAOQJL010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24846_length_2802_cov_3.801966"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PLG78_17220"
     CDS             complement(<1..1444)
                     /locus_tag="PLG78_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004485959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNE24916.1"
                     /translation="MTDPSFTFEQLAASAGIRTDGKSRPVVCVQGLGFVGAAMSVAVS
                     EAADPQGNPRFNVVGIDLPGPRGERVIRSLNEGKFPAETDDANLVAATSRAYRRGNLV
                     ATADAEAFRLASVVIVDINLDLGPTSADGEPFVDFSGLRAAIRTFATRISPDCLVLVE
                     TTVPPGTCAHVVAPEIRACFAERGLDPDSFLLAHSYERVMPGNQYLSSITNFWRVFAG
                     YNEAAARKCEEFLLTVVNAEDFPMSRMDTITASETAKVLENSYRATNIAFIEEWGRFA
                     ESVGVDLFQVLDAIRMRPTHNNIRQPGFGVGGYCLTKDPLFARFSATTIFKRPELDFP
                     FSRRAVAVNNQMPLVSVGRLTGMFGGDLRGRKVLLLGVSYRPDVGDTRYSASEIFARA
                     LVEAGASVVYHDPLVHDWPEMNAVVLQKLPPASGFDAIVLAVAHREYRDFDFASWIGE
                     NRPKILDTNNVLSRAQIESLVARQHEVKSIG"
     gene            complement(1441..>2802)
                     /locus_tag="PLG78_17225"
     CDS             complement(1441..>2802)
                     /locus_tag="PLG78_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HNE24917.1"
                     /translation="EPSPSVPSRNRLFQMDVTTWRERLALRWNMFIGRLFPRETIPVL
                     TLANADVAFHRRGFFLRKDIKRVSDHWTWPDVSMDQDRRIELFTALARSEAADKLFSF
                     CGLSGEQRAIARSLLVRFFCDFFPAQFLEGVGKNHAIADDLLREFQTRALISSGDGSS
                     HSMFVIGAARARGFKIVKFQHGGHYGYYRDFTHMLEVEFPDTDIFLSWGWTRLPEHPE
                     FSSLQVLPMPGPWMSERRLYWSKYRIGLGRRFDMLWMPQMMKRFPGAPQGASSVRRDV
                     LQSFSAEMIDVARHANERKVRIHCKPYNALTLYLMRETYAQLETVGGEYFDRAEHFDK
                     GLTMETLNHASLVLWDQPGTGFLECLQCGIPTMILWSRLYCEEEEWCVGDFKALEDAG
                     IVHRTASTLFDEYARFKLDPHAWMKKNSSVVSRFVARYGHAVNHWSSSWSHLLKQLRR
                     GKL"
BASE COUNT          750 a          766 c          682 g          604 t
ORIGIN      
        1 ttccaataga cttaacttca tgctgccggg caaccaggga ctcgatttga gctcttgata
       61 gcacattgtt tgtatccaga atcttaggcc gattctctcc aatccaggaa gcaaaatcaa
      121 aatcccgata ctcgcgatgg gctacagcaa gaacaattgc gtcaaaacct gatgccgggg
      181 gtagcttttg aagaacgact gcattcatct ccggccagtc atggaccaga ggatcgtgat
      241 aaacaactga cgcgccagcc tcaacaagag cgcgtgcgaa gatttcggaa gctgaataac
      301 gagtatctcc tacatctggc cgataactta cacccagcaa gagaaccttt ctccctcgca
      361 aatctccgcc gaacatacct gttaggcgcc cgacactgac gagaggcatt tgattgttca
      421 ccgcaacagc ccgacgtgag aaagggaaat ccagttcagg gcgcttgaaa attgttgtcg
      481 cagaaaaccg cgcgaacaat ggatccttgg tcaaacaata cccaccgaca ccgaatccag
      541 gctgacgaat attgttatgc gttggccgca tgcggatggc atcaagcacc tgaaacaaat
      601 caacgccaac actttcagca aagcgacccc actcttcgat gaatgcaatg ttcgtagctc
      661 gataactatt ttctagaacc ttggcagtct ccgacgctgt aatcgtgtcc atgcgcgaca
      721 tcggaaaatc ctctgcattg accactgtta gcaaaaactc ttcgcatttg cgagcagcag
      781 cttcgttgta tcctgcaaag acacgccaga aattcgtaat cgaggatagg tactgatttc
      841 cgggcatgac tctctcgtat gaatgagcaa gtagaaagct gtctggatcc aatccgcgct
      901 ctgcaaaaca agcccgaatc tcgggcgcta ccacgtgtgc gcaggttccg ggtggaactg
      961 tcgtttctac cagaacaaga cagtcgggcg aaattctagt tgcaaacgta cgaatggccg
     1021 cgcgcaatcc ggagaaatct acaaatggct ctccatctgc agaagtcggg ccaagatcaa
     1081 gattgatgtc cacgatcaca acgctcgcga ggcgaaaggc ttccgcatcc gccgtggcaa
     1141 caagattgcc gcgtctgtat gccctggaag ttgccgctac aaggtttgcg tcgtctgtct
     1201 cagctggaaa cttcccctcg ttgagagacc gaatcactct ttctccgcgt ggaccgggca
     1261 ggtctattcc tacgacattg aagcgtgggt tgccctgagg atctgcggcc tctgacaccg
     1321 ccacagacat cgccgcgcca acgaagccca gaccctggac acagacgacg ggcctggatt
     1381 tcccatctgt gcgaatgcca gcagaagcag ctaactgctc aaaggtgaaa gaaggatctg
     1441 tcataatttc cctcgacgaa gttgtttcag gagatgcgac caggagctcg accagtgatt
     1501 cacagcatga ccatatctag ccacaaagcg agataccaca gaagaattct tcttcatcca
     1561 ggcgtgagga tctaacttga agcgtgcgta ttcgtcaaac aaagtggacg cagttctgtg
     1621 cacaatgccc gcgtcctcaa gtgctttgaa atctcctaca caccattctt cctcttcgca
     1681 gtagagtctc gaccatagaa tcatagttgg aattccgcac tgaaggcatt ccaggaaccc
     1741 ggtgcctggc tgatcccaga gaaccagcga cgcatgattg agcgtttcca tggtcagacc
     1801 cttgtcaaaa tgttctgcgc gatcaaagta ttcaccgccc accgtttcga gttgcgcata
     1861 agtctctcgc atcaaataca atgtcagagc gttgtacggt ttgcagtgaa tgcgaacctt
     1921 tcgctcattg gcgtgccggg ccacatcgat catttccgca gagaatgact gcagcacgtc
     1981 gcgacgcacg gaagaagccc cctgaggagc gccaggaaaa cgtttcatca tttgcggcat
     2041 ccagagcatg tcaaagcgtc gacccagacc aatgcggtat ttcgaccagt agagtctacg
     2101 ttctgacatc cagggaccag gcatgggcaa tacttgaagt gaagagaatt ctggatgctc
     2161 tggaagcctt gtccatcccc agctcaagaa gatgtcggtg tcggggaatt cgacttccag
     2221 catatgcgta aaatctctat aataaccgta gtgtccaccg tgctgaaact tcacgatctt
     2281 gaaacccctg gcgcgcgccg cgccgatcac gaacatagaa tgcgacgatc catccccgga
     2341 tgaaatcaac gcacgggttt ggaactcccg gagcaagtcg tccgcgattg catgattctt
     2401 accaacgcct tctaaaaact gcgcgggaaa aaaatcgcaa aagaatctta ccagaagaga
     2461 ccgcgctatc gctcgctgtt cccctgaaag tccgcagaaa gagaataatt tatccgcagc
     2521 ttcgcttcgc gccaacgcgg tgaagagctc aatgcgacgg tcctgatcca tggatacatc
     2581 cggccatgtc cagtgatcag aaactctctt gatgtctttt cgaagaaaga atccgcgcct
     2641 atgaaatgca acgtccgcgt tcgcaagtgt cagtacaggg atggtttcgc gaggaaacag
     2701 gcgaccgatg aacatattcc atcgcaaagc aagccgctct cgccaggtag tgacgtccat
     2761 ctgaaagagc cgattgcgcg aaggaacgga aggagaaggt tc
//
LOCUS       DAOQJL010000518         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_24866_length_2800_cov_2.984699, whole genome shotgun sequence.
ACCESSION   DAOQJL010000518 DAOQJL010000000
VERSION     DAOQJL010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24866_length_2800_cov_2.984699"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLG78_17230"
     CDS             complement(<1..510)
                     /locus_tag="PLG78_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNE24918.1"
                     /translation="MLSTKTQRFWRVSALAYSSLLSFYVIGGALIARDLWVDHNPYSP
                     SGRIVVGSILKLSIDEPMTVEYEFQGANSEEAKVKMTPDKSLTEFLPPANVDKNTQNK
                     QDSRARSRARVKFRMGVQVTRVTETGIEFTGNKMVAPEEGRTRFQVQVQGQVNREDIQ
                     TDRTIKSEDV"
     gene            complement(473..1396)
                     /gene="flgA"
                     /locus_tag="PLG78_17235"
     CDS             complement(473..1396)
                     /gene="flgA"
                     /locus_tag="PLG78_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNE24919.1"
                     /translation="MIRFLILVLVCSSDLFAFKLFLEPVVRTQGGPVRLSDVSRSEGD
                     SPKDKVILGSIREAYFLSRDEIQRAAGTDCEAVYGPGVWVVPLTKTQDAEEAVESMKG
                     AIRRLSGGEEFLKKHTIKVSAGLKMPEQGASAVYRLPPQANKLAAGRMIVAVDLIVPD
                     NGKDRTLLRQQVELVIYRQVKVAVATHTLDQGDVIAHGDFKMETRELDSDVEKFAPEN
                     LVGQRLIDRVDAGKPIYASQLRKTSTVRRGQSITLIHQTPGIVVRCKSTSLTDAEPGG
                     RIKVRILLPSGKKTDIKEADVVDENTAVLAR"
     gene            complement(1393..2190)
                     /gene="flgG"
                     /locus_tag="PLG78_17240"
     CDS             complement(1393..2190)
                     /gene="flgG"
                     /locus_tag="PLG78_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNE24920.1"
                     /translation="MLRSLWTSASGMVAQQYQIDTIANNLANVNTFGFKKNRADFEDL
                     VYQHQVLAGTPATSVSEIPTGVYVGHGTRVAATQKMFEMGSLQATGNKFDMAVGSEVG
                     FFKVLMPDGNFAYTRDGSFKIDSRQQAVTSNGYLLEPPITLPPGAINNTFQVSENGEA
                     TVRIGDDQRPTRIGQIDLYRFVNPAGLQAIGKNLFKETIASGQEIQGTPGMDGMGGLM
                     QGFVEMSNVKLVEEMVNMIVAQRAYESNSKSIITSDSMLGTAIGMKR"
     gene            2292..2495
                     /locus_tag="PLG78_17245"
     CDS             2292..2495
                     /locus_tag="PLG78_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24921.1"
                     /translation="MNLTRIASARLLCKLARHAALICAILIVTRPSEMRARQPELAAA
                     VAVAAMSHLIGKRLESREKRHAQ"
     gene            2485..>2800
                     /locus_tag="PLG78_17250"
     CDS             2485..>2800
                     /locus_tag="PLG78_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNE24922.1"
                     /translation="MLSEFIGKDRIVPGLAGNMEGVTHALIERLPEDLRAQAAEKSLS
                     TNPFHYDGVAIPHFRSSSVASHEVVLGFAPAGIDMPQGKVRIILLLVLPAEDAGIAFL
                     QGM"
BASE COUNT          619 a          793 c          671 g          717 t
ORIGIN      
        1 cacatcttct gatttgatgg tccgatccgt ctggatgtct tcgcggttca cctgtccctg
       61 gacctgcacc tgaaaacgag tgcgcccttc ttccggtgcc accatcttgt tccctgtaaa
      121 ttctatcccg gtttctgtga cacgagtgac ctggacgccc attctaaatt tcactcttgc
      181 gcgactgcgt gctcttgagt cctgtttgtt ctgggtgttc ttgtctacgt ttgctggagg
      241 aagaaattca gtcagagact tatctggagt catctttact tttgcttctt ctgaattcgc
      301 gccctgaaac tcgtactcca ctgtcatggg ttcgtcaatg gacaatttca aaatagagcc
      361 gactacaatt cgtccggatg gactgtatgg attgtggtcc acccacaggt cgcgggcaat
      421 caaagccccg ccaatcacat aaaatgaaag aagagaagaa tacgcaagag cgctaacgcg
      481 ccagaaccgc tgtgttttcg tcgacaacat ccgcttcttt tatatcggtc tttttgcccg
      541 aaggcagtag aattcgaacc ttgatgcggc cacctggctc tgcatccgtg agggacgtgg
      601 atttacatcg aaccacgatt ccgggggtct ggtgaatgag agtgatgctt tgccccctcc
      661 gaactgtgga tgttttacga agctgggacg cataaattgg ctttccggcg tctacccgat
      721 cgatcagcct ttgcccgacc aagttctctg gtgcgaattt ctccacgtct gagtccagct
      781 cacgcgtctc catcttaaag tcgccatggg caatgacatc tccctgatcc agagtatggg
      841 tggccacggc caccttcacc tgtctataga tcaccagctc tacctgctgc ctgagtagag
      901 tccgatcctt cccgttgtct ggaacaatca gatccaccgc cacaatcatg cgtcctgcgg
      961 ccagtttgtt tgcctgaggt ggtaagcgat acactgcact ggcgccctgc tccggcatct
     1021 tgagcccggc actcaccttg attgtatgtt ttttcaagaa ttcttcgccg ccgctgagtc
     1081 tgcgaatcgc gcccttcatg gactcgactg cttcttctgc atcttgcgtt tttgtgaggg
     1141 gaactaccca gacaccgggt ccgtagaccg cttcgcagtc tgtgcctgcc gctctctgga
     1201 tttcgtccct ggaaagaaag tatgcttctc tgatagatcc taaaattact ttatctttcg
     1261 gagaatcccc ttcgctccgg ctgacatctg acagtcgcac cggtccgccc tgagtgcgga
     1321 ccactggttc tagaaagagt ttgaatgcga ataaatcaga actgcaaacc agaaccagga
     1381 ttagaaatcg aatcatcgct tcatgccaat ggcagttcca agcatgctgt cagacgtgat
     1441 gatggacttg gagttggatt catacgcgcg ctgagcgacg atcatgttaa ccatctcttc
     1501 taccagcttc acgttcgaca tttccacaaa cccctgcatc aatccgccca ttccatccat
     1561 gccaggagtt ccctggattt cctgtccgct tgcgatcgtc tctttaaata gattctttcc
     1621 gatcgcctga agaccggccg ggtttacaaa acgatataga tcaatctgac ctatgcgcgt
     1681 tggcctttga tcgtctccaa tgcgcacagt cgcctctcca ttttcagata cttgaaacgt
     1741 attattgatg gcacctggtg gcaacgtaat cggaggctca agcaagtacc cattggatgt
     1801 aactgcctgc tggcgcgaat cgatcttgaa actaccatct cgagtgtacg cgaaatttcc
     1861 atccggcatg aggaccttga agaatcccac ctctgaacca acggccatgt caaacttgtt
     1921 gccagtggcc tgaagacttc ccatctcaaa cattttttgt gtggccgcaa cacgcgttcc
     1981 atgacccaca tagacgcctg taggaatttc agaaacggag gtcgcaggag ttcccgcaag
     2041 cacctgatgc tgatagacaa gatcctcaaa atcggcccgg ttcttcttga aaccaaacgt
     2101 atttacgttg gccaggttgt tggcgatcgt atcgatttga tactgctgcg ccaccatacc
     2161 gctggcggat gtccatagag atcgaagcat agtcctccta tcggcctgcc ctgccgttcc
     2221 atgagcgata tcatggtctg accgacacaa aaaagctgga gcgacagtgg ccattccgcc
     2281 attcaatcca catgaacctg acgcgtatcg cctccgctcg cctcctgtgc aaactcgcca
     2341 gacacgcggc gctcatctgt gccattctca tcgtgaccag accctctgaa atgagggcca
     2401 gacagcctga actcgccgcg gccgtggccg tggctgcgat gtctcacctg attggcaaac
     2461 gtctcgaaag cagagagaaa agacatgctc agtgagttta ttggaaaaga tcggattgta
     2521 cccggactgg cgggcaacat ggaaggagtc acgcatgcgt tgatcgagcg acttccagaa
     2581 gatcttcgcg cacaggcggc tgagaagagc ctttccacca atccgtttca ttacgatgga
     2641 gtggccattc ctcactttcg atcgagctca gttgcctccc acgaggtagt cctcggattt
     2701 gcgcctgccg gaatcgacat gccccagggc aaggtccgca tcatactgct tctggtgttg
     2761 cctgcagaag acgcgggaat cgccttcttg caggggatgt
//
LOCUS       DAOQJL010000519         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25022_length_2791_cov_4.055190, whole genome shotgun sequence.
ACCESSION   DAOQJL010000519 DAOQJL010000000
VERSION     DAOQJL010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25022_length_2791_cov_4.055190"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLG78_17255"
     CDS             complement(<1..651)
                     /locus_tag="PLG78_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNE24923.1"
                     /translation="MNVVILLVPDAPASVVTGLSEFFEIAGLDMNERRAKRPCRVRTA
                     SVQADRVPLRGKVIIEPDIVGRMTEPCDLVIVPALGPNVAGIRRRLGMEIEWLKDAAS
                     RGTRIASVCSGAFLLASTGLIDDREATTHWLFAPLFRRMFPRVKLNIDRLVIDTGKFL
                     TSGGSNAFYDLALHVIEQSLGRDTALSCARHFLLDTNRVSQTPFIAFSAQKHHGDAG"
     gene            809..1207
                     /locus_tag="PLG78_17260"
     CDS             809..1207
                     /locus_tag="PLG78_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNE24924.1"
                     /translation="MLLAARIIAGIVGLLHVYIFMMESVWWMRPAIFKRFGVPDAKQA
                     EIMKSVFLNQGFYNLFLAIGALYGAICFELHPMYAPAIMIFACLSVFGAGTVLLVSAG
                     KRMLRAAIMQGLPPAISLALLASVLLRTAG"
     gene            1279..1932
                     /locus_tag="PLG78_17265"
     CDS             1279..1932
                     /locus_tag="PLG78_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE24925.1"
                     /translation="MQTVLITGANRGIGRATADALVARGCKVYYAARSIENLPSGDLA
                     TPISLDVTDPTSIARAVVFFEERKEALDVLINNAAIIGRADVTSFDVEEITRVMDTNF
                     NGPVRVTKAFFPFLEKSPSARVIHVSSGMGAISDLAGDHAAYRFSKSALNAFTISLAR
                     AQDRVKVVAVCPGWVRSDMGGKSAPRSLEEGAVGIVGLALNQIESGKFYRDGRVIPW"
     gene            complement(1949..>2791)
                     /locus_tag="PLG78_17270"
     CDS             complement(1949..>2791)
                     /locus_tag="PLG78_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24926.1"
                     /translation="AIDLTGRVGGLDSSVSAGPLFRFGSAPNTPAQGTAIYDDPSPLA
                     PPAESETYVYIEPSIKYQGRNGTLAGGSRGLSTSDLRTLVSNVSPNSIIASPLYGQIS
                     NEHTGSDIRRFVAFQTAVNQEPSIGWNYLIFNSIFNGGPTIDRDVRDAILRDILTSGM
                     NAADYPLAPFFAYDTLFRKPQKPQSVLTKYLAYVYLFQGRPLSAENLVAATALLWNER
                     DGSRRYAVQPRHVQGRFSIGMVFNRPGWFLQAGATISSLEYHSDSGVTPFHRYATLQF
                     GTRF"
BASE COUNT          654 a          692 c          754 g          691 t
ORIGIN      
        1 tcctgcatct ccatggtgct tctgagcgct aaatgcaatg aaaggtgttt gagagactct
       61 gtttgtatcc agaaggaagt gtctcgcgca cgaaagagca gtgtctcttc ccagggactg
      121 ttcaatcacg tgaagggcca gatcgtagaa ggcattgctt ccgcccgacg tcaggaattt
      181 ccccgtgtct atgaccagtc tgtctatgtt caatttcaca cgggggaaca ttctgcgaaa
      241 taaaggggca aacagccagt gagttgttgc ttctctgtcg tctatgaggc cagtggatgc
      301 gagcagaaac gctccagaac aaacactggc aatgcgggtg ccacgactgg ccgcgtcctt
      361 cagccattcg atttccatgc ccagtcttct cctgattccg gccacattcg ggcccagggc
      421 tggcacgatc accagatcgc aaggttctgt cattcgtcct acaatgtcag gctcgattat
      481 gaccttccct ctcagtggaa ctctgtctgc ctggacggat gcggtcctga cccggcaagg
      541 tctcttcgct cttctttcat tcatgtccag cccggcgatt tcgaaaaact cggacagtcc
      601 agtgaccacc gacgcaggcg catccggcac aagcaaaatc actacattca cgctccgatc
      661 gaggcatccc ggtggcggat tacaactgtt taatgcgatt aggccgaata attgatggca
      721 taatcacgat gaaaattggc atcaatcgag ctagcatcct gtgccactct gtggtaggat
      781 gagctcatca tttttcagga gaactcagat gttattagca gccagaatca tagcgggcat
      841 cgttggattg cttcacgtat acattttcat gatggaaagc gtgtggtgga tgagacctgc
      901 catcttcaaa agatttgggg tgcctgacgc aaagcaggca gagatcatga agagcgtatt
      961 tttgaaccag ggattttata atctatttct cgcaattggc gctctgtacg gagccatctg
     1021 ttttgaactc catccgatgt atgcgccggc gatcatgatt ttcgcatgtc tatctgtgtt
     1081 cggcgcgggc actgtgctcc tggtttctgc gggcaaaaga atgctgagag cggcgatcat
     1141 gcaaggcctt ccaccggcga tttcgcttgc actgcttgcc tctgtgcttc taagaaccgc
     1201 aggttgatcc tgggtcctgc gagggcgcaa gtgcgccttc gtatggacaa tcgctttcgt
     1261 ttgtgtttta ttctttgaat gcaaacagta ctcatcacag gagcaaatcg aggaataggt
     1321 cgagccacag cggacgctct ggtagcacgc ggttgcaaag tgtactatgc ggccagaagc
     1381 atagaaaacc taccgtcagg cgatcttgcc accccgatat cactcgatgt aacggatccc
     1441 acgtccattg cgcgcgcagt tgttttcttt gaggaaagaa aagaagcact cgatgttctc
     1501 atcaacaacg ccgccatcat aggaagagcg gatgtgactt cttttgacgt tgaagagata
     1561 acacgagtca tggatacaaa tttcaacggc ccggtgcgtg ttacaaaagc attctttccc
     1621 tttctggaga aatctccttc cgcgcgcgtc atccatgttt cgagtgggat gggcgctatc
     1681 tctgatcttg caggcgatca tgccgcctat cggttttcca aatctgccct gaatgcgttt
     1741 actatttctc tggctcgcgc tcaggaccgg gtaaaggttg ttgcagtctg tccgggatgg
     1801 gttcggtcag acatgggtgg gaagagcgcg ccgcgcagtc tagaagaagg tgctgttgga
     1861 attgtcggtc ttgcactcaa ccagatcgaa tcaggtaaat tttaccgcga tggtcgggtg
     1921 attccctggt aaacgtttgt gtcagcttct agaaccgcgt tccaaattgc aatgtcgcat
     1981 aacggtgaaa cggagtaacc cctgaatcag aatgatattc gagagagctt atggttgcgc
     2041 cagcttgcag aaaccaaccc ggccgattga ataccatacc aatagaaaat cgaccctgaa
     2101 catgccgcgg ctggactgcg tatctcctgg agccatcgcg ttcattccag agaagagcgg
     2161 tggcggcaac cagattctcc gccgacaatg ggcgtccctg gaagagataa acatacgcca
     2221 gatacttcgt caggacggat tgtggttttt gaggcttacg aaatagagtg tcgtacgcga
     2281 aaaaaggtgc cagcggataa tcggcggcat tcattccgct tgtgagtatg tcgcggagga
     2341 ttgcgtcgcg cacatctctg tcaatcgttg gcccgccgtt gaaaatagag ttaaagatga
     2401 gatagttcca cccaatggat ggttcctggt tcactgcggt ctgaaatgcg acaaagcgac
     2461 ggatgtcgct tcccgtgtgt tcgttcgaga tctggccata gagtggactg gcgatgatgg
     2521 agtttggaga cacattcgat acgagcgtcc tcaaatcgga agtggagagg ccgcgcgaac
     2581 ctcctgccag ggttccgttt cgcccctggt acttaatcga tggctcaata tagacgtacg
     2641 tttcggactc agccggaggt gccagtggag aaggatcatc atatatggcg gtgccctggg
     2701 cgggagtgtt gggtgctgac ccaaatcgga agagtggtcc ggcagaaacg gaagaatcaa
     2761 gtccaccgac tcgtcccgtt aagtcaatgg c
//
LOCUS       DAOQJL010000520         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25042_length_2790_cov_3.954296, whole genome shotgun sequence.
ACCESSION   DAOQJL010000520 DAOQJL010000000
VERSION     DAOQJL010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25042_length_2790_cov_3.954296"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2641
                     /locus_tag="PLG78_17275"
     CDS             <1..2641
                     /locus_tag="PLG78_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNE24927.1"
                     /translation="TTNILPFPLDESTGGFTDFYRRRVVLPFQGDPAVMRHVLDHELV
                     HAFQFDILQGERLGSMPLWLMEGMAEYLSSGWDQTAETYVRDAVLNNRMPELVDLHNE
                     SVNDAYAYYKGGQAVMMFIADTYGIDRIPFLLRAAHNTNRIDEAIRLTFKTSANEFNL
                     AFARYLRARYALASRAYAETKDLRMREVTDRYESQTGFQIRPAVSPDGQSIAYLTVDG
                     IFPAIVIRPAPGPGITKKRQETKKIVLRALRSSDYEEVQLLTTKLSYSPDGKSLMVAG
                     RRQGQQSILFFDVDSGDLIESESHALPLDSIAFPSMSPDGSKIVFSGTISGKTDLYIL
                     EKRSGMLRRLTSDARYESSPVFSNAGDQIYFSASSESKIVSSQIYRFDLASGKVFQIT
                     NIPGQNGHAISRQDGSILFLSTQDGVSNVFLLEHASTLTQPSSEVSAITASLTGIFDI
                     AGAGEKGIVFTERVEGASEVRLMPDVRTTQKLDENLITFHAKPAPMMELERQSAIRQI
                     GGEYSPFLAIEGMPFVFVTAAGGPDGKTSVAALGFMSLADESGDHRLSTLVSYNARPA
                     ELNLNLEYAYLKYRTDFFVGAYRQSGIFAVFNFLDFSLNSLLYNPYFRVLDQNTTGAY
                     GGFEYPLHSFGSISLVMSAGREERIYDTVRPEEKQQPDLFQNHQSLGFAYQYNNIVYS
                     MYGPLDGHAFLFQYSVPVQFNGRDRELYVTTTEFRFHHLFDNYSSLALRAFGATASGR
                     DASLYPFQIGGYYTIRGYRFLEFEGKHAFVMNLDYHFNFIEALRFGVPFSWSPGPVRG
                     ALFFDAGSAFDRPQDFQGYSRKSGTTRDLHLSFGAGIHWANFLWFLIPGALMKIEWAT
                     PYDTKRSLPLSKWKGAFSVGFNF"
     gene            complement(2600..>2790)
                     /locus_tag="PLG78_17280"
     CDS             complement(2600..>2790)
                     /locus_tag="PLG78_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24928.1"
                     /translation="PDIQDLKDSGNRAGAVVCWSIESNKLIVYAEFRTPQTSRSLEER
                     HAAFLSKVKANGESTFPF"
BASE COUNT          713 a          716 c          654 g          707 t
ORIGIN      
        1 caccaccaac attctgccgt ttccactgga tgaatccacc ggaggcttca cagatttcta
       61 cagacgtcgc gtggttcttc cttttcaggg agatccagca gtcatgcgac atgttctgga
      121 ccatgaactg gtacacgcat ttcaatttga cattctccag ggtgaacgtc tgggctccat
      181 gcctctctgg ctcatggagg gaatggcaga atatttgtcg agcggctggg accagactgc
      241 ggagacctac gttcgcgatg ctgtgctaaa caatcgcatg ccggagctcg tagacctgca
      301 caatgaatca gtaaacgacg catacgcgta ctacaaagga gggcaggctg tgatgatgtt
      361 catcgcagat acatatggaa tcgatcgaat cccatttctt cttcgcgccg cgcacaacac
      421 aaatcgtata gacgaagcaa ttcgcctaac gttcaaaacc agtgcaaacg aattcaatct
      481 ggctttcgcg cgttaccttc gcgctcgtta cgcgcttgct tcgcgcgcct acgcagaaac
      541 aaaagatctt agaatgcgag aggtgacaga tcgatatgaa tcacagacag gctttcagat
      601 ccgccctgct gtttccccgg acggacagtc catcgcctat ctaaccgtag acggaatctt
      661 tccagccatt gtaatcaggc cggcaccggg gccaggaatt acaaaaaaga gacaggagac
      721 aaaaaagatc gtccttcgcg ccttacgctc cagcgattac gaagaagtgc aacttctcac
      781 aacgaagctt tcttattctc ctgatggaaa atctttgatg gtagcgggaa gacgtcaggg
      841 ccagcaatcg attttgtttt tcgacgtgga ctcaggcgat ctcatcgaaa gcgaatcaca
      901 tgcgcttcct ctcgactcaa tagctttccc ttctatgtcg cctgacggga gtaagatcgt
      961 attttctgga acgatttctg gaaaaacaga tctatacatt ttagagaaaa ggtcaggcat
     1021 gctcaggcgc ctgacctctg atgcgcgcta cgaaagcagc ccggtgtttt cgaatgcagg
     1081 agatcagatc tacttcagtg cttcttctga aagcaaaatc gtgtcctcac agatttacag
     1141 attcgacctt gcgtccggga aagtttttca gatcacaaac ataccaggac aaaatggcca
     1201 cgcaatcagc cgacaggacg gaagcattct gtttctgagc acgcaagacg gagtatccaa
     1261 cgtcttcttg ctcgaacatg cttccactct tacgcagcca tcctctgaag tgtcagccat
     1321 cacagccagc ctcaccggca tcttcgacat tgcaggtgct ggcgaaaaag gaatcgtatt
     1381 cacagaaaga gtagaggggg catctgaagt tagactgatg ccggatgtac gcaccacaca
     1441 gaagctcgac gaaaacctga ttactttcca tgcgaaaccc gcgcccatga tggagctcga
     1501 aaggcagagc gcgattcgtc aaattggagg agaatattca cctttccttg cgatagaagg
     1561 aatgccattt gtttttgtca ctgcagcagg agggccggat ggaaagactt ctgtggcagc
     1621 cctgggattc atgtctctgg cagacgaatc aggggatcat agactgtcca ctctcgtaag
     1681 ctacaatgct cgccccgcag aattgaatct gaacctggaa tatgcgtatt tgaaatatcg
     1741 cacggacttc ttcgtgggcg cctacagaca gagtggaata ttcgcagtct tcaactttct
     1801 ggacttcagc ctcaatagct tgctttacaa tccgtacttt cgcgttctcg atcagaatac
     1861 gaccggtgcc tacggcggat ttgaatatcc actgcatagc tttggttcca tttcccttgt
     1921 catgagtgca ggccgcgaag aaagaatcta cgatacagtg cgaccggaag aaaaacaaca
     1981 gccagatctt ttccagaatc atcaatctct tggatttgcc taccagtaca acaacatcgt
     2041 ttattctatg tatggtcccc tggacggaca cgcgtttcta tttcaatatt cggttcccgt
     2101 tcaattcaat ggaagggaca gagagcttta cgttaccaca accgaattca gatttcatca
     2161 tctgtttgac aattactctt ctctcgcctt acgtgcgttt ggtgcgaccg catccggaag
     2221 agacgcgtct ctctatccgt ttcaaattgg cggatattat accatacgag gatacagatt
     2281 tctggaattt gaaggcaagc atgcattcgt aatgaacctc gattaccatt tcaatttcat
     2341 agaagcattg cgttttggcg ttccgttcag ctggagcccg gggccggtgc gtggcgcgct
     2401 tttcttcgac gcaggatctg cgtttgacag acctcaggac tttcaaggct attccagaaa
     2461 atcaggaacc actcgtgact tgcatttatc gtttggggct ggaattcact gggcgaattt
     2521 cctctggttt ctcataccgg gcgcgctcat gaaaatcgaa tgggccaccc cctatgatac
     2581 aaaaagatct ctgcctcttt caaaatggaa aggtgctttc tccgttggct ttaacttttg
     2641 agaggaatgc cgcatgacgt tcttccaggc tccgactcgt ctgcggcgtg cgaaactccg
     2701 cgtagacaat cagtttgttt gactcgatcg accagcatac aaccgcaccc gcgcgatttc
     2761 ccgaatcttt gagatcctgt atatcgggat
//
LOCUS       DAOQJL010000521         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25141_length_2784_cov_3.932942, whole genome shotgun sequence.
ACCESSION   DAOQJL010000521 DAOQJL010000000
VERSION     DAOQJL010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25141_length_2784_cov_3.932942"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..424
                     /locus_tag="PLG78_17285"
     CDS             2..424
                     /locus_tag="PLG78_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE24929.1"
                     /translation="MDMVAVMVAQRHCGREAVTASVDRLNFLSPISVGDHVLLRASVN
                     YTGRTSMEIGVQVSRENPYTGEVVRATTAYLTFVALDENKKPVPIPPVEPETEIEKRR
                     FRNAILRVEANRNLVKKMREEMQSSPLPDSSGASPQEK"
     gene            540..950
                     /locus_tag="PLG78_17290"
     CDS             540..950
                     /locus_tag="PLG78_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNE24930.1"
                     /translation="MCKREVAELLRSHDINPTTQRIEIAHLIFQKAQHLCADEILAEL
                     NKEYEQVSQATVYNTLKVFVDKKVVRELVFAPDRIYYDSNTSHHHHFIDVDTGKIYDV
                     SGDCVKCPELSYVDHLKAEVLEVSLILKGRMPQA"
     gene            1009..2334
                     /locus_tag="PLG78_17295"
     CDS             1009..2334
                     /locus_tag="PLG78_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNE24931.1"
                     /translation="MDDALLIARLQFAFTITFHYLFPQLTMGLALLILVQKAMFMFTR
                     QLSYNHAAQFWARIFAVNFAMGVVTGIPMEFQFGTNWARFSVFAGGVIGHVLAMEGVF
                     AFFIESAFLGLFLFGERRLGQRAHTFTAFMVFLGSWMSGYLIIITNAWMQHPVGFEAA
                     SGKLELVRFADLFFNPWAGWQYLHTMFGAVVTASFVMAATGAYYLLSNRDIRYGEIFL
                     RVGVTCGLAASVLMIFPTGDGQGKNVAQYQPVTLAAMEGLFETGKAAEMILIGQPDME
                     RLRIDNPIRIPGVLSLLTYSRWNAEVKGLQEFPRELWPTNIPLLYYSYHIMVGLGTYM
                     AAVMALCAVFLFRRHLFDHKWLLWILMLSFPAPYIANTAGWMTAELGRQPWLIYGILK
                     TTAGVSPTVSAGNSLFTLMGFLGMYGLLGTLFLFVIAREIQNGPEHGHH"
     gene            2336..>2784
                     /gene="cydB"
                     /locus_tag="PLG78_17300"
     CDS             2336..>2784
                     /gene="cydB"
                     /locus_tag="PLG78_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNE24932.1"
                     /translation="MQIETFWFIALVFTLTMYVVLDGFDLGVGILHLTVAKTDDERRT
                     LFNAIGPLWDGNEVWLIAAGGVLFFAFPVAYASSFSGFYLPLMMILWLLMFRALAIEF
                     RRHIENELWQQFWDVAFCVSSLGLVLLLGMALGNVLRGVVFGEDHFF"
BASE COUNT          622 a          666 c          720 g          776 t
ORIGIN      
        1 gattgatatg gtcgcggtga tggtggccca gcgccattgt gggcgcgaag ccgtcactgc
       61 gagtgtggat cgtctaaatt ttctttctcc catttctgtg ggcgatcatg tccttcttcg
      121 cgcaagcgtg aattacaccg gtcgtacttc tatggagata ggcgttcagg tttcgcgtga
      181 aaatccttat acaggggaag tggttcgcgc aaccaccgcg tacttgacct ttgttgctct
      241 ggacgaaaac aagaagccag tgcccatccc tcctgtggag cctgaaacag aaatagagaa
      301 aagacgattt cgaaacgcca tcctgcgcgt agaagcaaac agaaatctgg taaagaaaat
      361 gagggaggaa atgcaatcct caccgcttcc ggattcatcg ggtgcctctc cacaggaaaa
      421 atgagacata gtttgctttc cggataggcc ctgaggccta aaaaaggggt tgtcatccct
      481 aaatcccctg tctaagttga tagcggttac tgccgatgga agaccagaag aagtatggca
      541 tgtgcaagcg agaagtggcg gagctgcttc ggtcgcatga catcaatcct actacgcagc
      601 gcattgaaat cgcgcatttg attttccaga aagcccagca cctttgtgca gatgaaatcc
      661 tcgcagaact caacaaagaa tacgagcaag tcagccaggc aacagtatac aacactctga
      721 aagtatttgt agataagaaa gtagtccgcg agctagtatt tgcgccggac agaatctact
      781 atgattccaa cacaagccat catcaccatt tcatagatgt agacaccggt aagatttatg
      841 acgtttccgg agactgcgtc aaatgccctg agctcagcta cgtagatcac ctcaaagcag
      901 aggtgcttga agtgagtttg atcctcaagg gtcgcatgcc tcaggcataa tcactcacgc
      961 atcgtctttt tgttttgact ctgaaacacg cttccaatag aaagcggcat ggatgacgcg
     1021 ctcttaatcg cccgcctgca gttcgcgttc accatcacgt ttcattatct tttcccccag
     1081 ctgaccatgg gccttgcact tttgatcctg gtgcaaaagg caatgttcat gttcacccgg
     1141 caactctcct acaatcatgc agcgcaattc tgggctcgca tctttgctgt aaattttgcg
     1201 atgggcgtgg tgaccggaat tccaatggaa ttccagttcg gaacaaactg ggccaggttt
     1261 tctgtcttcg cgggcggagt gattggccac gttcttgcca tggagggagt atttgcgttt
     1321 ttcatagaat ctgcgttcct gggtcttttt ctttttggag aaagacgcct tggtcagcgt
     1381 gctcacacct tcacagcatt catggtattc cttggatcct ggatgagtgg atacttgatt
     1441 atcattacga atgcctggat gcaacatccc gttggttttg aagctgcgtc aggtaagctt
     1501 gagcttgttc gatttgcgga tttgtttttc aacccctggg ctggctggca atatttgcac
     1561 actatgttcg gtgcagtggt cactgcatct ttcgtgatgg cggccacggg tgcgtactac
     1621 cttctctcaa atcgcgacat cagatacggc gaaatctttc tgagagttgg ggtcacgtgc
     1681 gggctggctg cctccgtgct tatgattttt ccgacgggcg acgggcaggg aaagaatgtg
     1741 gcgcagtatc aacctgttac tctcgccgcg atggaaggac tctttgaaac tgggaaagcg
     1801 gcagagatga ttttaatcgg acagccggat atggaacgcc ttcgcatcga caatccaatt
     1861 cgcattccag gagtactcag tctcctgacg tattcgcgct ggaacgcgga ggtaaaaggg
     1921 ctccaggaat ttccgcgcga actctggcca acgaacattc ctcttctcta ttatagctat
     1981 cacatcatgg ttggtctggg cacatacatg gccgcggtga tggcgctctg cgctgtgttt
     2041 ttgtttcgca ggcatctctt cgaccataag tggcttctgt ggattttgat gctttctttt
     2101 ccggcgcctt acatcgcgaa tacggcaggc tggatgacgg cggaactggg gagacaaccc
     2161 tggctcattt acggaatctt gaaaactacc gctggcgttt cgcctactgt gtccgcggga
     2221 aatagcctgt ttactctgat gggatttctt ggaatgtacg gactcctggg tactcttttc
     2281 ttgtttgtga ttgcgcgcga aatccaaaac ggcccggaac acggacatca ttgatatgca
     2341 gatcgaaact ttctggttca tcgctcttgt gttcactcta accatgtatg tggttcttga
     2401 tggctttgat ctgggcgtcg gcattcttca cctgacggtg gcaaaaacag acgatgagcg
     2461 tcgcactctg ttcaatgcga ttggtccact ctgggatgga aacgaggtct ggttgatcgc
     2521 ggcaggcgga gttttgtttt ttgcctttcc agtggcgtac gcgtctagct tcagcggatt
     2581 ttatcttccg ctgatgatga ttctgtggct tctcatgttt cgagctctcg ccattgagtt
     2641 tcgtcgtcat atagaaaacg aattgtggca acagttctgg gatgtcgcat tctgtgtttc
     2701 cagcctgggt ctggttctct tgctcggaat ggctctcgga aatgttcttc gcggtgttgt
     2761 tttcggagaa gatcatttct tctt
//
LOCUS       DAOQJL010000522         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25185_length_2781_cov_4.205429, whole genome shotgun sequence.
ACCESSION   DAOQJL010000522 DAOQJL010000000
VERSION     DAOQJL010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25185_length_2781_cov_4.205429"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLG78_17305"
     CDS             <1..455
                     /locus_tag="PLG78_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE24933.1"
                     /translation="ILDILKEEGLPAKNLELEITESLAMQDPALAIRMIRILRDAGIS
                     VAVDDFGTGHSSLSSLKRFNFSRLKIDRSFIQEIAVEPESRAIVRAILGISEALGLAT
                     VAEGVENAEQLDTLRELGCEDSQGFLLAIPMPAEDATRWMESHAPMNR"
     gene            824..2326
                     /locus_tag="PLG78_17310"
     CDS             824..2326
                     /locus_tag="PLG78_17310"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE24934.1"
                     /translation="MRGIVTLILCLFVTPIRAQTPIDIGDGMPLLSLAAISETHAGSP
                     LDSIDSVSQQDDWKGPGEEFRFGLRGEPVWIHFRVRNPSADPATAFVSIPAPWISHAQ
                     LFRSTEGAWVKTETGYAVPHAQWDVRSFYPSLRILLGSRENADLYLRIESNRVLNFPA
                     SIMDRSEFRQRDRARTTISGLMIGVFLALLSLACILLFVERNSAFTLYCLYLLFLAAG
                     LAAMMGNGIQFFASVEPQILSRIGIMAFPAARAILLLMIPFFCGRPIAGNQIIIRIAG
                     IEMICGLVLAAGGWTISAQISTAIAFLSTILEIGALAASLRAAATLPAWLWLIAAGAR
                     IPAYVFEFGYLPYSTAVVYFWIFLIPLDFLILADRIRMLRTEKSRYAKSRITGLDVSS
                     IVTKMEKILTIDQLLRDPTLRLPDLARALDIPAYQLSEILNNVVQKSFREYINGLRIQ
                     TAQRSFTVDPDADIDTVMRENGFRSRSVFNHAFKSQTGQTPVEFKRSSQK"
     gene            2471..>2781
                     /locus_tag="PLG78_17315"
     CDS             2471..>2781
                     /locus_tag="PLG78_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE24935.1"
                     /translation="MTRILGISCYYHDAAATLVQDGDIIAAAQEERFTRKKHDPRFPE
                     SAIEYVLSEGNISQAEIDIVAFYDKPFIKLERILESILQASPRGYRLWLAAMPSWIDH
                     K"
BASE COUNT          662 a          741 c          673 g          705 t
ORIGIN      
        1 agattctaga catcctcaaa gaagaaggct tgccggcaaa aaatttggaa ctggagatca
       61 cggagagcct cgctatgcaa gatccagcgc tcgccattcg aatgatcagg attttgcggg
      121 acgcaggcat cagtgttgcc gtggacgatt ttgggacggg tcactcgtcg ctctcctctc
      181 tcaaacgttt caatttctca cgtctgaaaa ttgaccgctc cttcattcag gaaattgcag
      241 tagagccaga aagtcgcgcc attgttcggg ccatcctcgg aatctcagaa gcccttggcc
      301 tggccacagt cgcagagggc gtcgaaaatg cggagcaact tgatacgctt cgtgaacttg
      361 gttgcgaaga ttctcaaggc tttctcctgg ccatcccaat gcctgccgaa gatgcaacgc
      421 gctggatgga atcccacgca cctatgaacc gttaggcgac attattctgt ctgggttttc
      481 ctcgaagtgt cgaaccggca cacaccgtct acaagggccg gcaatcctcc gattcttttt
      541 gcatgggcat gcctggaatg ccgcgccaga agatacggac gcagccacgt tcacgcccag
      601 cgacaaaccc aatccttgga tcgcggattg attgcacaca aaacgaatga gaaagaaggg
      661 agcagcccgc gtggccggag gaaaaaacca gcgaccaacg gatgcgcgca agaaagatca
      721 gccgcgcttc tgctcggatc tggaaaatag aggaacttaa tactcgatct cacatttgtt
      781 agggagttgc aaaaccagga tcggcgcctt aagatggtgc tttatgcgcg ggatagtgac
      841 gctcattctg tgcctgttcg tcacgcccat ccgcgcacag actccaatag acattgggga
      901 cggcatgcca cttctttctc tggccgcgat ttcggaaaca cacgctggat cgccactgga
      961 ctcgatcgat tcggtttctc agcaagacga ctggaaggga cccggtgagg aattccgttt
     1021 tggattgcgg ggcgagccgg tctggattca ctttcgtgtt cgcaaccctt ccgcagatcc
     1081 agctactgcc tttgttagca tacccgcacc ctggatcagt cacgcccagc tctttcgttc
     1141 gactgaaggg gcttgggtaa aaacagaaac aggttatgcc gtcccgcacg cgcaatggga
     1201 cgtgcgcagc ttctaccctt cccttcgcat tttgctggga tcaagagaga atgcagatct
     1261 ctatctgcgc atcgaatcca atcgagttct gaattttcca gcctccatca tggatcgttc
     1321 agaatttcgt cagcgtgatc gtgcccggac gacaatcagc ggactgatga tcggtgtatt
     1381 ccttgcgctg ctttccctcg cgtgcatcct gctttttgtc gagcgcaaca gtgccttcac
     1441 actctattgt ctctatcttc tcttcctggc ggcaggactc gcggctatga tgggaaacgg
     1501 aattcagttt tttgcttccg tagaaccgca gatactttcg cgcatcggca tcatggcttt
     1561 tccagccgcg cgagccatcc tgctgctcat gattccattc ttctgtggca ggccgattgc
     1621 aggaaatcag atcatcatcc gaattgccgg gattgaaatg atttgcggcc tggttctcgc
     1681 tgcaggtggc tggaccatca gcgctcagat ttcaactgcg attgctttcc tttccacgat
     1741 tcttgaaatc ggtgctcttg ccgcttcttt gagggctgcc gcaactctgc cggcatggct
     1801 ctggctcatt gcagcgggtg cgcgcattcc agcatatgtt ttcgaatttg gatatcttcc
     1861 ttactccact gcggttgtct acttctggat cttcctcatt ccgctcgatt tcctaattct
     1921 tgccgatcga attcgcatgc ttcgcacaga aaaaagccgt tatgcgaaat caagaatcac
     1981 tggtctggat gtttcttcca tcgtgacgaa aatggagaaa attcttacaa tagaccaact
     2041 attacgagat cctacactcc gccttcctga cctcgcaaga gcgctggata tccccgctta
     2101 tcagctctct gaaattttga acaatgtggt tcaaaagtcc ttcagagaat atataaatgg
     2161 actacgcata cagactgctc agcgatcatt cactgtggat cccgatgcag acatagacac
     2221 ggtgatgcgc gaaaatggat ttcgatcacg ttctgtcttt aaccatgctt tcaaatctca
     2281 aaccggacaa acaccggtcg agttcaaacg ctcttcacag aagtaactca gaagatgtct
     2341 aaattataaa ctcgtaagag tttcacgcat cttcgcacgg cgcacgcatg atcttttttt
     2401 tgtctccgtc ctggtcgttg aaaagaaaaa gactggattt caaaataaag tcaggattct
     2461 cattcatccg atgacgagaa tactcggaat ttcttgttac tatcacgacg cggccgcaac
     2521 gctggtgcaa gatggcgata tcattgcggc agcacaagag gagcgcttca cgcgcaagaa
     2581 gcatgacccg cgattcccgg aaagcgcaat tgaatacgta ctgtctgagg gaaacatctc
     2641 gcaggctgaa atcgacatcg ttgctttcta tgataagccc tttatcaagt tagagagaat
     2701 ccttgaatcg attctgcagg catccccccg cggatataga ttgtggctgg cggcgatgcc
     2761 atcctggatc gatcataaat t
//
LOCUS       DAOQJL010000523         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25254_length_2777_cov_4.699118, whole genome shotgun sequence.
ACCESSION   DAOQJL010000523 DAOQJL010000000
VERSION     DAOQJL010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25254_length_2777_cov_4.699118"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="purH"
                     /locus_tag="PLG78_17320"
     CDS             <1..933
                     /gene="purH"
                     /locus_tag="PLG78_17320"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772433.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNE24936.1"
                     /translation="ERRTGGLWHQIQGKELSYNNMLDANAALMSALSLPGHGVVIVKH
                     LNPCGVALASMDPALGIFGATPGQEGSANVQTFQGGMPEAFRLARACDPVSAFGGVIA
                     LTDLVDLETANLINENFAEVIIAPGYSPEALDAFSAKKNLRLLEIKRPERFLAPGMEL
                     RLNELGLLYEDMDHTYAPRSTWKVVTNRAPEAAELNALELAWRVVKHVKSNAIVFTNE
                     RSTLGIGAGQMSRVDSTEIAASKAKKAGLSLATSCVASDAFFPFRDGVDALARAGARA
                     VIQPGGSVRDEEVIQACNEHGIAMVFTGMRHFRH"
     gene            1037..1933
                     /locus_tag="PLG78_17325"
     CDS             1037..1933
                     /locus_tag="PLG78_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24937.1"
                     /translation="MNSCIALQKLELFQQGKNSFGVAQSAFLQGDAKLIDQFISQSEA
                     NVDLARYFLGVQKPEDSISIASSLKADALYIIFKRDYAVFLQKQKQKAPADRETNNYF
                     LQKATQYWSKLPVAKICTVIAYIVREKKDVSSAAQFMVLIPSSVISRFPELSGLTEDE
                     ERELFLGLEGDLFTLPLLSPGIYDHMMTLFAEQPEISMVLAGMEELVKRRSVVISTTD
                     NMVEYYNKTNSRLSLPALYSELFGIEFELAAEVLHHLVEHQVITTNERNTLLEFLKSG
                     KLDFIRQMREDILSVSPLPRTD"
     gene            complement(1930..>2777)
                     /locus_tag="PLG78_17330"
     CDS             complement(1930..>2777)
                     /locus_tag="PLG78_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNE24938.1"
                     /translation="LFLFIIDSIKMAEDFALIFKNLRQYLESNNIRYAEVFLAPSRMI
                     QNGLDYRDIADSLDSLSRECRNSGGPDVRFLIDVSRTFGPENASKNLQRVLSNPRPSI
                     IGIGLGGAELMGPARDYRDVFAQAQAEGLRTVAHAGEDDGPWSIRDTVELLKAERIGH
                     GTSAVQDPALLELIRKKAIPIEICLTSNIFTGKYVRREKDHPVRRYYEEGIICTVNTD
                     DPEIFHVTLSDEYFKFHKSLNFTVSEIVDLCRQGVYATFQHNPATMWNQFKPQIDDLR
                     KKFAL"
BASE COUNT          732 a          695 c          682 g          668 t
ORIGIN      
        1 gaaagaagaa ccggtggcct gtggcatcaa attcaaggca aagaactgtc gtataacaac
       61 atgttagacg cgaacgcagc tctgatgagc gccctttccc tcccgggaca tggagttgtc
      121 atcgtaaagc atttgaatcc ttgcggagtc gctcttgcat ctatggatcc ggcgctcggt
      181 atctttggcg caactccggg gcaggaagga tcggcaaacg ttcagacttt tcaaggtgga
      241 atgccagaag cgtttcgcct tgcgcgcgcc tgcgatccag tcagcgcgtt tggcggagtg
      301 atcgctctta cggatctggt ggatctggaa acagcaaatc tgatcaatga gaattttgcg
      361 gaagtgatca tcgcgccagg gtactctcca gaagctctgg atgcgttttc cgcgaagaaa
      421 aatctaaggc tcctcgaaat caagagacca gagcgattcc tcgctccggg gatggagctt
      481 cgcctgaatg aactcgggct actctatgaa gacatggatc acacctacgc acccagatct
      541 acctggaagg tcgttacgaa tcgggcgccg gaagccgcag aactcaatgc cctggagctt
      601 gcctggagag ttgtgaagca cgtaaaatcc aacgcgatcg tctttacgaa tgaaaggtcc
      661 acacttggaa ttggggcggg tcagatgagt cgggtcgatt caactgaaat tgctgcctcc
      721 aaagctaaaa aggcggggct ttctctggcc acgtcctgtg ttgcttcgga tgcattcttt
      781 cctttccgtg acggagtgga cgctctggct cgcgcgggag cacgcgccgt gatccaaccg
      841 ggtggatctg tgagagacga agaagtgatt caggcttgca acgaacacgg cattgctatg
      901 gtatttaccg ggatgagaca tttccgacat tgatatggac ccgcataagc gatgcctgtt
      961 agatacaggt ctgcaccaga agtgcctggc aaattgcaaa tctgcaattc aggacaacag
     1021 gtggcagtcc aatctcatga actcgtgcat cgcgctacaa aagctggaac tcttccaaca
     1081 aggtaaaaat tcattcggtg tagcacaaag tgcgttcctc cagggagacg caaagctcat
     1141 agatcaattc atttctcagt cggaggccaa cgtagacctc gcgcgctatt ttcttggcgt
     1201 acagaaacca gaagattcca tcagcattgc aagctccctg aaagcggatg ctctctatat
     1261 aatattcaaa cgcgactacg cagtttttct gcaaaagcaa aaacaaaagg cacctgcaga
     1321 ccgcgagacc aacaactact tcttacagaa agccacccag tactggtcca aacttcccgt
     1381 cgccaaaatc tgtacggtca ttgcctacat cgtgcgagag aaaaaagacg taagtagtgc
     1441 ggcgcaattt atggtgctta ttccgtccag cgtcatctcc cgctttccgg aactctccgg
     1501 cttgacagaa gatgaagaaa gggaactatt cctggggctg gagggagatc ttttcacctt
     1561 accgctgtta tcccctggca tatacgatca catgatgacc ctgtttgcag aacagccgga
     1621 aatttccatg gtgctcgcgg gaatggaaga gctcgtaaaa agacgatccg ttgtgatatc
     1681 aactacagac aatatggtgg agtattacaa caaaaccaat tcacgacttt cccttcctgc
     1741 cctctattcc gaactatttg gaattgaatt cgaactcgcg gcggaagtgc ttcatcatct
     1801 ggtggaacat caggtcatca cgacaaacga aagaaatact ctgctggaat ttttgaaatc
     1861 gggcaagctc gacttcatcc gacaaatgag agaagacatt ttgtccgtct caccgcttcc
     1921 gcgcacagac taaagtgcga atttttttcg aaggtcgtct atctgtggtt tgaactggtt
     1981 ccacatcgta gctggattgt gctgaaacgt agcatacaca ccctggcggc atagatccac
     2041 aatttcactc accgtaaaat tcaacgactt gtggaatttg aaatactcat ctgaaagagt
     2101 gacgtgaaag atctccgggt catccgtatt caccgtgcaa atgatccctt cttcatagta
     2161 acgacggaca ggatgatctt tctcacgacg aacatatttg cctgtgaaga tattgctggt
     2221 caggcaaatc tcaataggaa ttgccttctt acgaatcaat tcaagcagtg ctggatcttg
     2281 aactgcggat gtgccatgtc cgattctctc agctttcaaa agttctacag tgtcccgaat
     2341 ggaccatggg ccgtcatcct caccggcgtg cgctaccgtg cgaagccctt cggcctgggc
     2401 ctgtgcgaag acatctctgt aatcacgcgc cgggcccatg agctctgctc cgcccagccc
     2461 aattccaatg atagaagggc gcggattgga caatactctt tgcaggtttt tactcgcgtt
     2521 ttctgggcca aatgtgcgcg aaacgtcaat gaggaaacga acgtccggcc ctccgctgtt
     2581 tcgacattct cgcgaaagcg aatccagtga gtccgcaatg tcccggtaat ccaatccatt
     2641 ttgaatcatt cgcgagggag caagaaatac ttccgcataa cgaatgttat tcgattcaag
     2701 atactgcctg agattcttga agatcaatgc gaagtcttcg gccatcttga tagagtcgat
     2761 aatgaaaaga aagagtt
//
LOCUS       DAOQJL010000524         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25393_length_2768_cov_4.430520, whole genome shotgun sequence.
ACCESSION   DAOQJL010000524 DAOQJL010000000
VERSION     DAOQJL010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25393_length_2768_cov_4.430520"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLG78_17335"
     CDS             complement(<1..201)
                     /locus_tag="PLG78_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE24939.1"
                     /translation="MAKKEIAGLSNLLPARKKAKAKRAKAAVSIAPPEGSRKEGKRIG
                     RGRATGAGKTSGRGQKGQKARAG"
     gene            complement(215..403)
                     /gene="rpmD"
                     /locus_tag="PLG78_17340"
     CDS             complement(215..403)
                     /gene="rpmD"
                     /locus_tag="PLG78_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNE24940.1"
                     /translation="MGEQVTITLVKSPVGRVPKHRGTLRALGLWKIGEKRSHKMTPQL
                     AGMIRTVSYLITVESAKK"
     gene            complement(403..903)
                     /gene="rpsE"
                     /locus_tag="PLG78_17345"
     CDS             complement(403..903)
                     /gene="rpsE"
                     /locus_tag="PLG78_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNE24941.1"
                     /translation="MEKEFSEKVVKINRVSKVVKGGRRFSFNALAVVGDNHGRVGIGL
                     GKANEVPDAIRKAIESARRQLRHVRLTRKNTIPHEVVGRFKATRVTLLPATPGTGIIA
                     GEAVRAVVEQAGIHDVLSKVIGSKNPVNVVRATLDALEQMETPIMSARKRGVSLQHIF
                     GGKGAA"
     gene            complement(945..1307)
                     /gene="rplR"
                     /locus_tag="PLG78_17350"
     CDS             complement(945..1307)
                     /gene="rplR"
                     /locus_tag="PLG78_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNE24942.1"
                     /translation="MNRIAQKQERLGRRRDRVRFAIRSRKARPRLVFNRSNRFLAVQL
                     IDDAKGVTLAHASTMEEAFKSGGTKNKAAAKKLGELMADRILKLGIKQIVLDRRGILY
                     HGKIAEFADAARAKGLEF"
     gene            complement(1311..1862)
                     /gene="rplF"
                     /locus_tag="PLG78_17355"
     CDS             complement(1311..1862)
                     /gene="rplF"
                     /locus_tag="PLG78_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNE24943.1"
                     /translation="MSRIGNAPIQLPQGVDVEASGDKLKVKGPLGTLESPLPAGITLS
                     KEGGVVNLKRANDEMPLKQKHGMARAILMNSVIGVSKGWEKKLELVGVGYRAQMKGAD
                     LSFSLGYSHEVKFQVPAGIKVQVTDQVRLDITGIDRQKVGQTASEIRSLRPPEPYKGK
                     GIKYAGENIRRKAGKTGKAGKGK"
     gene            complement(1876..2271)
                     /gene="rpsH"
                     /locus_tag="PLG78_17360"
     CDS             complement(1876..2271)
                     /gene="rpsH"
                     /locus_tag="PLG78_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNE24944.1"
                     /translation="MSLSDPIADMLTRIRNASRAKHPKVTFGGSRLKIAILDILKREG
                     FIEDYAVRKDKQKSELDVMLKYSGRQPVIRQLQRVSSPGRRFYIKATEVRPVRNHLGL
                     SILSTPKGVMTGREARTQNVGGELICRVW"
     gene            complement(2291..2476)
                     /locus_tag="PLG78_17365"
     CDS             complement(2291..2476)
                     /locus_tag="PLG78_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNE24945.1"
                     /translation="MAKKSLREKHKRKQAFKVREYNRCPVCGRPRAYMRRFDMCRICF
                     RKLAGFGQIPGVIKSSW"
     gene            complement(2484..>2768)
                     /gene="rplE"
                     /locus_tag="PLG78_17370"
     CDS             complement(2484..>2768)
                     /gene="rplE"
                     /locus_tag="PLG78_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE24946.1"
                     /translation="VTMRGDRMFEFLERFITIALPRVRDFKGLNPSGFDGRGNYNLSL
                     KEQIIFPEIDVDKVQNYHGMNVTIVTTAPTNEEAFALLEAIGMPFSKGKK"
BASE COUNT          594 a          781 c          645 g          748 t
ORIGIN      
        1 gccggcacgc gctttctggc ctttctggcc acgacctgaa gtcttgccgg caccggtggc
       61 gcggccacgt ccgatgcgct tgccttcttt acgagatcct tccggtggag caatgctcac
      121 ggctgccttt gctcttttcg ccttcgcttt tttacgagca ggaagcagat tggaaagccc
      181 tgcgatttct ttctttgcca ttgttaatat atccttactt tttcgctgat tcaaccgtga
      241 tcaaataaga cacggtgcga atcataccgg ccagctgcgg agtcattttg tgactgcgct
      301 tttcgccgat cttccagagt cccagtgcgc gaagcgttcc acgatgcttg ggcacgcgac
      361 caaccgggct cttaacaaga gtgatggtga cttgctcgcc cattatgccg ctcccttacc
      421 gccgaagatg tgctggagag acacgccgcg cttgcgcgca gacatgattg gagtctccat
      481 ttgttcgagt gcgtccagag tagcacgcac cacattcaca ggattcttcg agccgattac
      541 tttgctcagt acgtcatgaa ttccagcctg ctctactaca gcgcgcaccg cttctccagc
      601 gatgatccct gttccaggcg ttgcaggcaa aagtgttacg cgggttgctt tgaaacgacc
      661 cacaacttcg tgaggaatcg tgttcttgcg agtcaaacga acatgacgca attgtctgcg
      721 cgcagactcg atcgccttac gaatggcgtc cggtacttcg ttcgctttgc ccagaccaat
      781 tccaacgcgg ccgtggttgt ctcccacgac tgcaagagcg ttgaaagaga atctacgacc
      841 gcccttcaca acttttgaaa cgcggttgat ctttacaact ttctcggaga attccttctc
      901 catttcctga tgctggtgtt gatgtctttc tctttcgctc ataattaaaa ctccagtcct
      961 tttgcgcgcg ccgcatctgc aaactcagca atcttgccgt gatacaaaat gccgcgacgg
     1021 tccagtacga tctgcttgat tcccagcttc aaaattcggt ctgccataag ctcgcccagt
     1081 ttctttgccg cagccttgtt ctttgttccg ccagacttaa acgcttcttc cattgtgctt
     1141 gcatgagcga gagtaacgcc tttcgcatca tcaatcagtt gaaccgcgag gaagcgattg
     1201 gaacgattga aaacgagtct tggacgcgct ttgcgagaac gaatagcgaa acggacgcgg
     1261 tcacgacggc gcccaagtct ttcctgtttc tgtgcgatgc gattcatggc ttatttacct
     1321 ttccccgctt taccggtctt accagctttg cgacggatgt tttcacccgc atacttgatc
     1381 cccttccctt tgtaaggttc tggtggacgc agcgaacgaa tttcagatgc agtttgaccc
     1441 actttctggc ggtcaattcc agtaatatca agacgaacct ggtcggttac ttgcactttg
     1501 attccggcag gaacctgaaa ctttacttca tgcgaatacc caagtgagaa actcagatcc
     1561 gcacctttca tctgcgcgcg ataaccaaca ccaacgagct ccagcttctt ttcccagccc
     1621 ttgctcactc cgatcacgga gttcatgagg atagcgcgag ccatgccatg cttttgcttc
     1681 aatggcatct catcgttggc tctcttgaga ttcaccactc cgccttcttt gctcagcgta
     1741 atgccggcag gaagaggtga ttcaagagtc ccgagcggac cttttacttt cagcttgtcg
     1801 ccagatgctt ctacatctac gccctgaggc agctgaatgg gtgcatttcc aattctagac
     1861 ataccggtct cctgattacc acacgcggca gatcaattcg ccacccacat tctgtgtgcg
     1921 cgcttctctg cctgtcatca cgccttttgg cgtagagagg atcgaaagtc cgaggtgatt
     1981 tcgaaccgga cgaacctcag tcgctttaat atagaagcga cggcctgggc tggatactct
     2041 ttgcagctgg cgaatcaccg gctgacggcc agagtacttc aacattacat ccagctcgga
     2101 cttctgtttg tccttccgaa ccgcataatc ctcaatgaac ccttcgcgtt tgaggatgtc
     2161 gaggatcgcg atcttaagac gtgatccgcc gaacgtaacc ttaggatgtt tggcccgcga
     2221 cgcgttgcga attctcgtga gcatatctgc tattggatct gaaagactca tctatattac
     2281 tccaaatgtt ttaccaggac gacttgatga cgcctggaat ttgaccgaaa ccggccagct
     2341 tacgaaagca aatacggcac atatcaaagc gcctcatgta ggcacgcgga cgcccgcaca
     2401 ccggacagcg gttgtattcc ctcactttga aggcttgttt ccgcttgtgt ttttccctca
     2461 ggctcttctt tgccatgata ccctcacttc tttcctttgc taaacggcat gccaatcgct
     2521 tcgagcaatg cgaatgcttc ttcgtttgta ggcgccgtgg taacgatcgt aacattcatc
     2581 ccgtggtaat tttgaacttt atccacatcg atttctggga aaatgatctg ctctttcaga
     2641 gaaagattgt agttcccgcg gccgtcgaaa cctgatgggt tgaggccctt gaagtcgcga
     2701 acacgaggca gtgcaatcgt gatgaacctt tcaaggaatt caaacatgcg atcgccacgc
     2761 attgtgac
//
LOCUS       DAOQJL010000525         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25562_length_2758_cov_3.492416, whole genome shotgun sequence.
ACCESSION   DAOQJL010000525 DAOQJL010000000
VERSION     DAOQJL010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25562_length_2758_cov_3.492416"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLG78_17375"
     CDS             <1..461
                     /locus_tag="PLG78_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE24947.1"
                     /translation="RALFEFTDFDCAPCREVQETLRRAQAAQPVRLVFKSLPLADTEA
                     GRLPHLVAFCVHAQNKDAFWLYYDLMFSSSAPARDKDFLTTVSRTLDLDEEAMKKCVG
                     SEEAASALKKDRADAQAAGVAGTPTFFLGNARLEGNKSTAEFLAFVKANR"
     gene            complement(547..1542)
                     /locus_tag="PLG78_17380"
     CDS             complement(547..1542)
                     /locus_tag="PLG78_17380"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNE24948.1"
                     /translation="MNKKVIIFGAIVAVLVVGVIYLLMPSSSDKSGNRPPAFFEMFSR
                     SGGEGQSSVVMAGSSIDYSEFVQKLKTGDVNFVWEVWAMRAKCAEETSAQDCDASILS
                     YIEKHYSSPEKEKMLSLFKKYFQYEAEMRDTDLGANSSFNEKYDTMKKKRRDILGKED
                     AELIFGMEEAQVDFIGLSQRQINASKNLSGDERVRQYEALKKQTFGQYYDAEIKREDK
                     FQNYQTELTLRENDLAKLSDADRAKRTQAIQEQYFGKDAAAAIAKAQEEEAAEQKKLA
                     DFEQKQKDFLAQNAAMPAAEKAEKLKAIRIQILGEEEAAAYERRKAFEDAVSKIK"
     gene            complement(1639..2535)
                     /locus_tag="PLG78_17385"
     CDS             complement(1639..2535)
                     /locus_tag="PLG78_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HNE24949.1"
                     /translation="MKRLFLMAAMFAIATSLQAQALSGTCVVLVHGILGFDDTNGLAG
                     GLVKYWGGVDTYLRNQGAKVATPGSSATNDVPTRSAQIKTYLNTWMPANGCTKVHLVG
                     HSQGGLVSRYLVSNLGYGSKVLSVTTVDSLHKGAPMADIVLGVIPSWLQPFANSALSL
                     LARLVYRDGRPQDAIAMGKSLTVSYSNALNAQCPDVSGIKYSSYANHMAWADPIQHPI
                     MALTYPITWAGGIWYGLGGSNDGVVPLSSQKYGTYKGEASSKWYATGIDHLQATNLEW
                     SGQNYFDVNGWYLGIVKNARGY"
BASE COUNT          661 a          707 c          658 g          732 t
ORIGIN      
        1 cgagagctct tttcgaattc acagattttg attgtgctcc ctgtcgtgag gtgcaagaga
       61 ctctgagaag agcgcaggca gcacaacctg tgcgactcgt attcaaaagc ctgccgttag
      121 ccgacactga ggcaggaagg ctcccgcatc tggtagcatt ctgcgttcac gcccagaaca
      181 aagacgcatt ctggctttac tatgatctca tgttttctag ttccgcacct gcgcgggaca
      241 aagactttct aactactgtt tcgcgaaccc tggacctgga cgaagaagcc atgaagaagt
      301 gcgttggttc ggaggaagca gcatccgctc taaaaaagga tcgcgcagat gcacaggcgg
      361 ccggagtggc aggcactccc actttctttt tgggaaacgc tcgcctggaa ggcaataagt
      421 ccacagcaga attcctggcg ttcgtgaaag ccaatcggta ggacgcttca gaaaccctgc
      481 aatggaagag tggcagaagt gcgatcgccc gaagagctcg ggcgatcatt caaaaagagt
      541 tgagaattac ttgattttgc ttactgcgtc ctcaaacgcc tttcttcttt cgtaagcagc
      601 agcttcctct tcgccgagaa tctgaattct gattgccttc agcttttctg ccttctcagc
      661 tgccggcatg gcggcattct gtgccagaaa atccttctgt ttttgctcaa aatcagccag
      721 tttcttttgc tcggccgctt cttcttcctg agctttcgca atggctgcag ctgcgtcttt
      781 tccaaaatac tgttcctgaa ttgcctgcgt gcgctttgcg cgatccgcat ctgacagttt
      841 tgccagatcg ttttcgcgca gagtcagctc tgtctggtaa ttctggaact tatcttcgcg
      901 tttgatctcc gcatcgtaat attgaccgaa cgtttgcttt ttgagagctt catactgacg
      961 aactctttca tccccactca aattcttgga agcattgatc tggcgctgag aaaggccaat
     1021 gaaatcgacc tgagcttctt ccattccaaa gatgagctcc gcgtcttctt tgcccaggat
     1081 gtcgcgccgc ttctttttca tggtatcgta tttttcattg aagctagaat tcgcaccaag
     1141 atccgtatcg cgcatttccg cttcgtactg gaagtatttc ttaaaaagag aaagcatctt
     1201 ttctttctca ggtgagctgt agtgtttttc tatgtaagac agtatggatg catcgcagtc
     1261 ctgagcgctt gtctcttccg cgcatttcgc acgcatggcc cagacttccc aaacaaagtt
     1321 cacatcgcct gttttcaact tctgcacaaa ctcgctatag tcaatggacg accctgccat
     1381 tacaacagac gactgcccct caccgccgga gcgagagaac atttcgaaga atgcaggagg
     1441 gcgattcccg gatttatcag atgaggatgg catgagcaaa tagatcactc ctacaaccag
     1501 aacggcaacg atcgctccaa aaatgattac tttcttgttc atgtgtttcc tctataaata
     1561 gatcgggcca gcagttgctg gcccgacttg aattagattc tttcaattag attcttttta
     1621 agaaatcaat cgagtgaatc agtagcctct tgcgttctta acaatgccaa gataccagcc
     1681 gtttacatca aagtaattct ggccgctcca ctcgaggttc gtcgcctgca ggtggtcgat
     1741 gcctgttgca taccacttgg aagacgcttc gcctttgtat gttccatact tctgagagct
     1801 gagtggaaca acgccgtcat tggatccacc cagaccatac cagattccac ctgcccaggt
     1861 aattgggtaa gtgagagcca tgattggatg ctggattgga tctgcccatg ccatgtggtt
     1921 ggcgtagctg gagtatttga ttccagatac atcaggacac tgcgcattca gagcattgga
     1981 gtaagataca gtgagcgact tacccattgc aattgcatcc tgtggacggc catcgcggta
     2041 cacgagtctt gcaagcaggc taagcgcgct gtttgcgaaa ggctggagcc aggatggaat
     2101 gacaccaaga acgatgtctg ccattggcgc acctttgtgc agtgaatcaa cggtagtaac
     2161 agaaagaact ttggagccgt atccaaggtt tgaaaccagg taacgagata caagtccacc
     2221 ctgagaatgt ccaaccaggt gaaccttggt gcaaccgtta gccggcatcc atgtgttcag
     2281 gtaggttttg atttgcgcag aacgagtagg aacatcgttc gttgcagagc ttccaggtgt
     2341 agccaccttt gcgccctgat ttctaaggta agtatctacg cccccccagt acttaacgag
     2401 accaccggcc aggccgtttg tatcgtcaaa acccaggatg ccatgaacga gaaccacgca
     2461 ggttccggac agtgcttgag cttgtagtga tgtagcaatg gcgaacatag ccgccatgag
     2521 aaacagacgt ttcatttgat ttccccctgt acacattagg ccaaacgcat caggtttttg
     2581 tcaagcaaat gacgattcct gacataatgc aaggccctcc ccccggggcc gggggaccct
     2641 gggatcgccg ccattttttt catagtcgat tttcgaaata atcggacgtt tttcgggcta
     2701 gagattgatt ccggcatcgg atatgacgcg acgatcaatt cgcctctgca cttcttcc
//
LOCUS       DAOQJL010000526         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25572_length_2757_cov_4.629534, whole genome shotgun sequence.
ACCESSION   DAOQJL010000526 DAOQJL010000000
VERSION     DAOQJL010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25572_length_2757_cov_4.629534"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG78_17390"
     CDS             <1..317
                     /locus_tag="PLG78_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HNE24950.1"
                     /translation="PSCKEPFRASARDLASLGLSGAEAGKIYKHVGCENCVGTGYRGR
                     TGLYELMLMDEDVQRIVIRGGDSEAVKKVALEKGMQTLQTYGKNKVLDGVTTIEEVLR
                     VT"
     gene            320..1546
                     /locus_tag="PLG78_17395"
     CDS             320..1546
                     /locus_tag="PLG78_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE24951.1"
                     /translation="MPIYKYTAFNRKGKEEKGIVDAGNHAQARRILKQKGLYVKALTE
                     DTEKKDRELFPFLAKLLYRVPRRDVGFFARQLGTLLDAGLPLDKSLANIVDQTENEYL
                     KKALIEVRGDVIEGGKLSESMAKHPAIFPPLYHNLVSVGERTGTYEQSLLRLAELEEA
                     NQAMKNKVTAALFYPVIMMFFLGGIMIFLLAVVMPQIQQMFVQMNAELPFITRFVLGV
                     SDIFTSFKILIPIVSGIVGFILFRRWKSTVTGRMTWERFLLRVPVFGALIQKVMLARF
                     SRNLGVMLQSRVPLLNALIVVGKIVDNAVFEQEILAAIEKIKEGTRITDAFRDSQIMT
                     MMILGMLSAGEASDRIPEMVSKIADVLEDDVEAAIQKMSSLLEPLMIVLLGLMIIVIM
                     SAILLPMYNLTSQIQV"
     gene            1567..2031
                     /locus_tag="PLG78_17400"
     CDS             1567..2031
                     /locus_tag="PLG78_17400"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNE24952.1"
                     /translation="MNLNSLKKRRGMTLIELSIVIVVLGIIIGLVTYGLRTAGAGAQD
                     SANKLKVNQQATTLRLELNKYQDEYGSMQDGETLIKLTKRDSEHPNWTPLSEKDVLDP
                     WNEPYFLCRDDAGEQQICSRGADKKEGGQGKDADFMLTREDSYPQWLSGKKK"
     gene            1967..2578
                     /locus_tag="PLG78_17405"
     CDS             1967..2578
                     /locus_tag="PLG78_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNE24953.1"
                     /translation="MQTSCLRGKTRIRNGLAAKRSNLSLRRGLTLIELGVVMAVMGII
                     LTVLVSLYRQFSVFKSTQDEATMLRDALLFSRSTAIRSNDIIYVAFDLDEEKYHSYRK
                     VREQDEIKEKEVIKERSLSAANSLVAVQVGSSNRQDRGKLVVKMYPDGSSDELMIFLG
                     PGPGADIKYTVLFSRYGKAEAAKGEKEPAREDSTYKENLEDWQ"
     gene            2575..>2757
                     /locus_tag="PLG78_17410"
     CDS             2575..>2757
                     /locus_tag="PLG78_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNE24954.1"
                     /translation="MRSRRGMTLVEMGLAIAVAASMMMIVMMTVGSALRMQTESDRLA
                     VAVSLAQTKLSQLMSNP"
BASE COUNT          745 a          638 c          723 g          651 t
ORIGIN      
        1 gtccttcttg caaggaaccg tttcgcgcat cggcacggga tctggcaagc ctgggtcttt
       61 ctggtgcgga ggcaggaaaa atctacaaac atgtgggctg cgagaattgc gtgggcacag
      121 gataccgcgg aagaaccggt ctttacgaat tgatgctgat ggacgaagac gtgcagagaa
      181 ttgtaattcg cggcggagat tcagaagcag tgaaaaaggt tgcgctcgag aagggcatgc
      241 aaacactcca gacttacggc aagaataagg tactcgatgg agtcacaacc atcgaggaag
      301 ttctccgcgt gacctgatca tgcccattta caaatacact gcattcaatc gcaaaggaaa
      361 agaagagaag ggaattgtcg atgcaggcaa tcacgctcag gcacgccgta tcctcaagca
      421 gaaggggctc tacgtcaaag ccctcacaga agatacagaa aagaaggaca gagaactatt
      481 tccattcctg gcaaagcttt tatatcgcgt tcccaggaga gacgtaggat tcttcgcgcg
      541 ccagttaggt acgctgctcg atgcaggcct tcctctagac aagtcgctcg cgaacattgt
      601 agatcaaact gaaaatgagt atttaaaaaa agcactcatc gaggttcgcg gagatgtaat
      661 cgaaggcgga aaactttccg agtccatggc aaagcacccc gccatttttc cgccgctcta
      721 tcataacctg gtttccgtgg gagaaaggac aggaacgtac gaacaatctc ttcttcgcct
      781 tgctgagctg gaagaagcaa atcaggccat gaagaacaaa gtaacggcag ctttgtttta
      841 tccggtgatc atgatgtttt tcctgggcgg tatcatgatc tttctgcttg cagttgtgat
      901 gccccagatc cagcaaatgt ttgttcagat gaatgcagaa ctgccattca tcaccaggtt
      961 cgttctgggt gtatcagata tattcacttc gtttaagatt ttaattccca ttgtttcggg
     1021 aattgttggt ttcatcctat ttcgcagatg gaaatcaact gtaaccggtc gcatgacctg
     1081 ggaaaggttc ctgctgcgtg ttccagtttt tggagcattg atccagaaag taatgcttgc
     1141 tagattttcg cgcaacctgg gtgtaatgct ccagagtcgc gtgcctttgc tcaacgcgct
     1201 gatcgttgtg ggtaagattg tagacaacgc agtcttcgaa caggaaatcc tggcagccat
     1261 tgagaagatc aaagaaggta cgcgaatcac agatgccttt cgcgattctc agattatgac
     1321 catgatgatc ctggggatgc tttctgcagg cgaagcgtct gacagaatcc ctgaaatggt
     1381 atctaaaatc gcagacgttc tagaggacga cgtggaagca gccatccaga agatgtcttc
     1441 gcttctagaa ccactgatga ttgttcttct gggcctgatg atcatcgtca ttatgtccgc
     1501 gattctcctt cctatgtata acttgacatc acagatacag gtttgatgct gatgtgcgga
     1561 ggagatatga atctgaacag tctgaaaaaa cgcaggggta tgaccctcat cgaactttct
     1621 atcgtcattg tggttctggg gatcatcatt ggcctggtaa cctacggtct aagaacggcc
     1681 ggagcaggcg ctcaggacag tgcgaataag ctcaaggtaa accagcaggc caccactctt
     1741 cgccttgaat tgaacaaata ccaggatgaa tacggatcca tgcaagacgg cgaaaccctc
     1801 atcaagctga caaagaggga ttcagagcac ccaaactgga ctccactgag cgaaaaggat
     1861 gtcctggatc cgtggaatga accgtatttc ctttgccgcg acgatgcggg ggaacaacag
     1921 atttgtagcc gtggcgccga caagaaggaa ggcgggcagg gaaaagatgc agacttcatg
     1981 cttacgcggg aagactcgta tccgcaatgg cttagcggca aaaagaagta acctctcgct
     2041 ccgtcggggg cttaccctga ttgagctggg cgttgtcatg gccgtcatgg gaatcatcct
     2101 gacggtcctg gtatcgctgt atcgacaatt ttccgttttc aaatccacgc aggatgaagc
     2161 caccatgttg cgtgacgctc ttctcttctc tcgctccacc gccattcgtt cgaatgatat
     2221 catttacgta gcatttgatc tggacgaaga aaaatatcac tcctatcgca aggttcgaga
     2281 gcaggacgaa atcaaagaaa aagaagtgat caaagagaga agtctttccg ctgccaactc
     2341 tctcgtcgca gtgcaggtgg gatcttcgaa cagacaggat cgcggaaagc ttgtagtgaa
     2401 aatgtatccg gatggatcct ctgatgaact catgattttt cttggcccgg ggccaggcgc
     2461 tgacatcaaa tacaccgttc ttttttcgag atatggtaag gcggaagccg cgaaaggaga
     2521 aaaagaacca gcgcgcgaag actctacata caaagagaat ctagaggact ggcaatgaga
     2581 tccaggcgag ggatgaccct ggtggagatg gggctggcca tcgcggttgc agcaagcatg
     2641 atgatgatcg tgatgatgac cgtgggttct gctcttcgca tgcaaacgga atcagatcgc
     2701 ctcgcggttg cagtgtctct tgcgcagacc aaactatcgc agctgatgag caacccc
//
LOCUS       DAOQJL010000527         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25937_length_2735_cov_4.766791, whole genome shotgun sequence.
ACCESSION   DAOQJL010000527 DAOQJL010000000
VERSION     DAOQJL010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25937_length_2735_cov_4.766791"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLG78_17415"
     CDS             <1..248
                     /locus_tag="PLG78_17415"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HNE24955.1"
                     /translation="EGRLGNGIFGDENNETTRTPVQVKGIRDAVYHGMGHSHSCVAVK
                     DGRVFCWGLNDSGQLGNGQTKNSAEPVEVKGVVLNFQ"
     gene            295..1770
                     /locus_tag="PLG78_17420"
     CDS             295..1770
                     /locus_tag="PLG78_17420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNE24956.1"
                     /translation="MFKLHWLDLTLIGAYLALMVYMGKVLSTKIKSESDYYLAGKSLP
                     WYIIGLSIIGTNIGSNDYLGASGGAYRIGIAQANFEWIGAIPAMIISAFIFIPFYWRA
                     GVYSIPEYLGRRYNQPVRVISATILSLFAILIVGVYHWASALMLETYMGWPMYVSIPI
                     TAAVVGFYTISGGLRADTFTDAIQVTLMFVSSLFLAYFGVQKAGGPEAFLHTLSTKFP
                     GHMHAFLPSDHPDFPWPGVILGLGFVLSPAYWCANQAILLRTLGARSEWDGRASMIFA
                     ALAKTFVPVLIILPGFVALALSTEKLAVSDSALPWVVKNVLPPGPSGLFFVAFIAALQ
                     ASVSSALNSTAVMVTRDILGVLRPKPRTDAQVVRLGRICTFLVLALGVASAPLTAKFE
                     GIYIFVQTALSLFQGPMFALIVVGILYKYATPAAAVVSLVLGLLLAAILQIASFNMLY
                     IAFWTFVFSGFLLFVVSMFTARKPDEELVNLCYTTTVKKYV"
     gene            1763..1963
                     /locus_tag="PLG78_17425"
     CDS             1763..1963
                     /locus_tag="PLG78_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24957.1"
                     /translation="MFNWIHTLPLWTANALAMGLFGLILVICWSLPSAFVWSGSKRVF
                     YKDLRIWATLLTLIQIIIYCFV"
     gene            1988..>2735
                     /locus_tag="PLG78_17430"
     CDS             1988..>2735
                     /locus_tag="PLG78_17430"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNE24958.1"
                     /translation="MTELLRTGAAYYPDYIPEFVRTWRNGNVETISRRERMIQDFARM
                     KRNGFTWIRMGEFSWSLVEPEPGAFNPALFLEALDLALSHEIDVIFCTPTATPPPWLT
                     ERNPEILPRDKSGRLMPAGTRRHYDPTNAAYRLESRRITEYYARTFGNHPAVKCWQTD
                     NEFGCHGSTYVYTESAASWFRAWLKDNYLDIHSLNEAWQTSFWSQRYTDFSQIPLPLS
                     SYADANPGLELDFRRAMNHAWKIFQSEQAIL"
BASE COUNT          587 a          729 c          672 g          747 t
ORIGIN      
        1 atgaaggacg tctcggaaac ggaattttcg gggatgaaaa caacgaaacc accagaacgc
       61 cggtccaggt aaagggcatc agggatgctg tgtatcacgg aatggggcac agccattcct
      121 gcgtcgccgt taaagatggc cgcgttttct gctggggatt gaacgattct ggccagctcg
      181 gcaacggcca gacaaagaac tcagcggaac cggtggaagt caaaggtgtt gtcctgaatt
      241 ttcagtagcg gtaatcgcgc ctaatgaatg ttaggcgatc aaggggcaac gcatatgttt
      301 aaacttcact ggctggatct gacgctgatc ggggcgtacc tcgcactcat ggtctacatg
      361 ggcaaggtcc tatccacgaa aatcaaatca gaaagcgact actatctggc gggtaagtct
      421 ttgccctggt acatcatcgg gctttccatc atagggacaa atataggatc aaacgactat
      481 ctgggtgcga gtggcggagc gtatcgcatc ggaattgcac aggcaaactt cgagtggatc
      541 ggcgcgattc ccgcgatgat catctccgca ttcatattca ttcccttcta ctggcgcgca
      601 ggtgtttatt ccatcccgga gtatctgggt cgtcgctaca accagccggt gcgggtgatt
      661 tccgccacaa tcttgtctct attcgcgatc ttaattgtgg gcgtctatca ctgggcttcc
      721 gcattgatgc tcgaaacata tatgggctgg cctatgtatg tgagtattcc aatcacggcg
      781 gctgtggtag gattctatac tatttctgga ggtcttcgcg cagatacgtt cacagatgca
      841 atccaggtaa cgcttatgtt cgtcagctct ctgtttcttg cttacttcgg cgtccagaag
      901 gcaggcgggc cggaagcgtt tcttcatact ctctctacaa aattcccagg acacatgcat
      961 gcatttcttc catcggatca tcctgatttt ccgtggcctg gagtgatcct tggacttggc
     1021 tttgtactct ctccggcata ctggtgcgcc aatcaggcga ttcttcttcg cacactgggt
     1081 gcgcgctcag agtgggatgg tcgcgcatct atgatctttg ctgcccttgc caaaacattt
     1141 gtgcctgtgc tcatcattct accaggattc gtcgctctgg ctctctccac ggagaaactc
     1201 gctgtgagtg attccgctct gccatgggtt gtaaaaaacg ttctcccacc cgggccatcc
     1261 ggtctattct tcgtggcatt catcgcagca ctccaggctt ccgtgagttc cgcgctcaac
     1321 tctactgcag tcatggtcac tcgcgacatt cttggtgtgc ttcgtcctaa acctcgcaca
     1381 gatgctcagg tggtacgcct gggtcgaatt tgcacgtttc tggttcttgc gctcggtgtg
     1441 gcatctgcac cgctgactgc gaagtttgag ggcatctaca tctttgtgca gacagcactc
     1501 tctcttttcc agggtcctat gttcgctctc attgtagttg gaattcttta taaatatgcc
     1561 acacccgcgg cggccgtggt gtctctggtt cttggacttc tgctcgcagc catcttacag
     1621 attgcatcct tcaacatgct ctacattgct ttctggactt ttgtattctc tggcttcctg
     1681 ctttttgttg taagtatgtt cactgcgcga aagccagatg aagaacttgt gaatctttgt
     1741 tacactacca cggtgaaaaa atatgtttaa ctggatccat actctccctc tctggactgc
     1801 gaacgctctg gccatgggtt tgttcggatt gattctggtg atttgctggt ccctgccctc
     1861 cgcgttcgtc tggagtggtt cgaaaagagt attctacaaa gacttacgaa tctgggccac
     1921 tcttctgact ctcatccaga tcatcatcta ctgcttcgtg taaggtgcgt tccatgcaga
     1981 ctgcgttatg acagaactac ttcgcaccgg tgcggcgtac tatccggact atattccaga
     2041 atttgtgcga acgtggcgca atggcaatgt ggaaacgatt tcgcgtcgcg aacgaatgat
     2101 ccaggatttc gcgcgcatga agcgcaatgg ctttacgtgg attcgcatgg gagaattcag
     2161 ctggagcctc gtggaacccg aaccaggagc atttaacccg gctctttttc tagaagccct
     2221 ggatcttgcc ctttctcacg agatcgatgt aattttctgc actcccaccg caactccacc
     2281 tccctggctc acagaacgaa atccagaaat tctgccgcga gataagtccg gtcggctcat
     2341 gcccgcgggc acacgaaggc attacgatcc aaccaatgcc gcctatcgtt tagaaagtcg
     2401 tcgcatcacc gaatactatg cgcgaacatt tggaaatcat cctgcagtca aatgctggca
     2461 gacggacaat gaatttggat gtcacggctc tacttacgtt tacactgaat cggcagccag
     2521 ctggtttcgg gcatggctca aagacaacta cctggacatc cattctctca atgaggcctg
     2581 gcaaacatct ttctggtcac agcgctacac cgatttctcg cagatcccgc ttcctctctc
     2641 atcttacgcg gatgcgaatc ccggcctcga acttgatttt cgccgtgcga tgaatcatgc
     2701 gtggaagatc tttcaatcgg aacaggccat ccttc
//
LOCUS       DAOQJL010000528         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_25986_length_2732_cov_3.611132, whole genome shotgun sequence.
ACCESSION   DAOQJL010000528 DAOQJL010000000
VERSION     DAOQJL010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25986_length_2732_cov_3.611132"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLG78_17435"
     CDS             <1..196
                     /locus_tag="PLG78_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNE24959.1"
                     /translation="NPEQLWETTMDPEKRVMLKVRPLSESEIGLDGIERTFDILMGDD
                     VEPRRRFIEQNARLVAHLDL"
     gene            complement(217..2409)
                     /locus_tag="PLG78_17440"
     CDS             complement(217..2409)
                     /locus_tag="PLG78_17440"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24960.1"
                     /translation="MRRILGCLLLLILGQTAASAVDLTRRDLYIREGFDLAWTTEFKP
                     EGWLRIPGEEDGTRPVRLSELSAGEKRTFFSIADHGIKTYTFVTDFPMSNEDLAGTKV
                     YALYLAQIGMNWEVYLNGKLLRSEMPPAPPGKIRNRTMRGVIIPIDSRLLKAGPNIIA
                     YHITGDPTFEDTGFYRSRPYTIDTIQNIEGLRYDPIMIVLLSLYGAVSIFHFVLFLRT
                     PRERFNLYYSLLTATLFFYYSLRTNSIFDIITDSSAIFLLDVSSVILIVPFGASFLDS
                     LFFYQVTRFTKGLWAFSLLMIALLFPFPLPFARDVLYIWQASLPVSLFYILYIIGGQF
                     LTKTREYETIYKLTPFRAGLRALLRSPPGNLLLGGFIVSGCALVEVIDSVTEARGVNA
                     VVYGLFCFVGGVAAILANRIIEVHRKVDLLNRDLSDKLQEIQKTSGMLRNSEEKYRQI
                     IEGSSDMIFTLDDDLRFTSVNRALHRDLGFPEEEVLGSRFFDLLYQGELESGLAVNLV
                     HRRFEEFKRNGKTLGMKIQLKSKYTQEPREFSARFERVQVEDGIQILGKATLVLEDSL
                     LRYFIKERQKYVIGNYLISAEELSQRLVRNVEKYLDPSRVTALRIGLREILINAIEHG
                     NLAITYDEKTRELTTGNYLEFIQARQLDPRFRDRKVVIEYELNTLFVQYTITDEGEGF
                     DWQKLKAEEDIHQQDSHGRGIAVTENAFDELIYSGKGNQVQLIKHFRR"
     gene            2504..>2732
                     /locus_tag="PLG78_17445"
     CDS             2504..>2732
                     /locus_tag="PLG78_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE24961.1"
                     /translation="MDERAVRLLVVEDDEITLGILREFLRGRGYEVRLAVSGEECIQS
                     LEREIPDLVLMDMHLPDLNGLQVLNQIRSNPR"
BASE COUNT          651 a          652 c          715 g          714 t
ORIGIN      
        1 gaatccggag cagctctggg aaaccacgat ggatccagag aaacgcgtga tgctgaaggt
       61 gcgtccactt tctgaatctg agattggcct ggacggaatc gaacgcacgt ttgatattct
      121 gatgggcgat gatgtagaac cacgcagacg cttcattgag cagaacgcca gactggtggc
      181 tcacctggat ctttgattgg atactgaacc ggcgcttcag cgccggaagt gtttgatgag
      241 ctgcacctgg tttcctttgc ctgaatagat caattcatca aatgcgtttt ctgtaactgc
      301 aattccccgg ccgtggctgt cttgctggtg aatgtcttct tctgccttga gtttctgcca
      361 gtcaaagcct tccccttcgt ctgtgatcgt gtactgcaca aacagagtat ttagttcgta
      421 ttcgatgacc accttacgat cgcgaaagcg agggtcgagc tggcgcgcct gaatgaattc
      481 gagatagttt ccggttgtca attcgcgcgt tttttcgtcg taggtgatgg ccagatttcc
      541 gtgttcgatt gcgttgatta gaatttcgcg caggccaatg cgaagtgcag tcacgcgcga
      601 aggatccaga tatttctcca catttcgcac tagtctctgg cttagctctt ccgcggatat
      661 caggtagttt ccgatcacgt acttttgtct ttctttgata aaatagcgaa gaagcgaatc
      721 ttccagaacc agagttgctt tcccaaggat ttgaattcca tcctctacct gcacccgttc
      781 aaatcgagcg ctgaattcgc gaggctcttg cgtgtatttg gacttcagct ggattttcat
      841 ccccagcgtt tttccatttc tcttgaactc ttcaaagcga cgatgaacca gattcacagc
      901 cagccctgat tcgagttctc cctggtagag tagatcaaaa aaccgcgagc cgagtacttc
      961 ttcctccgga aatcccaggt ctctgtgcaa agctctgttt acactcgtga agcgaagatc
     1021 gtcatcgaga gtgaagatca tgtcagagga tccttctatg atttgcctgt atttctcctc
     1081 tgaattgcga agcattccgg aagtcttctg gatttcctgc agtttatcgc tcagatcgcg
     1141 gttgagcaga tctactttgc gatgcacttc aatgatgcga ttggcgagta tcgcggccac
     1201 tccgcctaca aagcaaaaaa ggccataaac gactgcattt acgccgcgcg cttctgtgac
     1261 agagtcgatc acctccacga gcgcgcaacc gctgacgatg aatccgccca ggagtagatt
     1321 gcccggagga gaacgaagga gcgcacgcag ccccgcgcga aatggagtga gtttatagat
     1381 ggtttcgtat tcccttgttt tggtgaggaa ctgtccacca atgatataga ggatatagaa
     1441 cagcgataca ggaagggaag cctgccatat atagagcacg tctcgcgcga acggaagagg
     1501 gaaagggaac agaagtgcga tcatcaatag ggaaaacgcc cagagccctt ttgtgaatct
     1561 ggtgacctga tagaagaaga gactgtccag gaaactcgcg ccaaatggaa cgatgaggat
     1621 cactgaagag acatccagaa ggaagatagc gcttgagtct gtgatgatat caaagatcga
     1681 atttgtgcga agagaataat agaagaacag tgtggccgtg agaagagagt agtagaggtt
     1741 gaacctttcg cgcggagtgc gcagaaacaa cacgaaatga aaaatgctca ccgcgccata
     1801 aagactgagt agaacgatca tgattggatc gtagcgaagc ccttctatgt tctggatagt
     1861 gtcgattgtg taagggcggc ttctgtagaa tccagtgtct tcgaacgttg gatctcccgt
     1921 tatgtgatag gcgatgatgt tgggccctgc tttcagaagt cgcgagtcga tggggatgat
     1981 tactcctcgc atggttctgt tgcgaatttt ccccggaggt gccggtggca tttctgatct
     2041 caacaatttt ccgttgagat acacttccca gttcatgccg atttgcgcca ggtagagcgc
     2101 gtagactttt gtgccggcca gatcctcatt gctcatggga aaatctgtga cgaatgtgta
     2161 ggttttgatt ccgtgatctg cgatggaaaa aaacgtcctt ttttccccag cggagagctc
     2221 tgaaagccgc acgggccgag tcccgtcttc ttctcctgga attctcagcc agccttccgg
     2281 tttgaattca gttgtccagg ctaagtcgaa tccttctcga atgtagagat cgcgtctggt
     2341 gagatccacg gcggacgccg cggtctggcc gagaatcaag agcagcagac atcccaggat
     2401 ccttctcatc gtcttgttcg tctttggcgc ttccgggaac ctgggcaagc gattttgata
     2461 tttttgcatt tacgcggaca tccctgaccc aaacgtacgc accatggacg agcgtgcggt
     2521 tcgcctcctc gttgtagaag acgatgagat tacactgggc attcttcgtg agttcctgcg
     2581 cggtcgaggc tatgaagtcc gtcttgcagt cagcggtgaa gaatgcatac agagtctgga
     2641 gcgtgaaatt cccgaccttg ttcttatgga tatgcatctg ccggatttaa acggtctgca
     2701 ggttctcaat cagatcagat ccaatcctcg tt
//
LOCUS       DAOQJL010000529         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26065_length_2727_cov_4.473428, whole genome shotgun sequence.
ACCESSION   DAOQJL010000529 DAOQJL010000000
VERSION     DAOQJL010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26065_length_2727_cov_4.473428"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG78_17450"
     CDS             <1..237
                     /locus_tag="PLG78_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE24962.1"
                     /translation="AYFKQGKYAPAIRYFQDCIDLDPDFPESHFNLAILYMKLGQPGP
                     ARAYFEAYLDLDPDSEWGELARQFLEDIQSAAEE"
     gene            complement(240..761)
                     /locus_tag="PLG78_17455"
     CDS             complement(240..761)
                     /locus_tag="PLG78_17455"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNE24963.1"
                     /translation="MTLFTLQSSILEIILRTGIVFAALMIFVRIFGKKEIGQIAPNDL
                     VLLLLISNAVQNAMVGADQSVPGGLAAVGTLLALNFLLTFLASRSKSFRSIVEGKAAI
                     LIHRGTVVSENLKKEKVSILELEQALREHGVAAISDVYLAVLELDGSISVLKKDEIPA
                     ETKPHHRMRFLKR"
     gene            1438..1932
                     /locus_tag="PLG78_17460"
     CDS             1438..1932
                     /locus_tag="PLG78_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24964.1"
                     /translation="MKANVFTRLAVTFAAFCAIGLLANPIRIWEPALIILLGERSNVG
                     RLQQWIALKGGIWPFLAGFLSVEIHWLEGFAFVVLALLLAGAFARSINGCLWISAACS
                     ILAAAWPGYLRPVVFGGIHRFDEISTYITIFLRMESGFFLLLILFLHALHSRRRGRVS
                     HFDS"
     gene            1922..2248
                     /locus_tag="PLG78_17465"
     CDS             1922..2248
                     /locus_tag="PLG78_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24965.1"
                     /translation="MTVSLWTGSPGAAADSICAEAARDDHGVLSPNIGGLTVSSNGNV
                     NGTIGTDTPYGAVGVSITNGQLGASLSGDLGNFNYANGQIGGNVNAGPFGLGIHDGQL
                     TQFLEY"
     gene            2250..2660
                     /locus_tag="PLG78_17470"
     CDS             2250..2660
                     /locus_tag="PLG78_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24966.1"
                     /translation="MGFSAYVNKSAESMGEWLKLGAFEQDANSGEWYPLDERLIKQLP
                     SRYWNAYEGYIGNGSGRAELLDDLAKVSDTFPTIERLLKCDTVIRPDQLDAFSREVER
                     LLSDRITRHLRLIALRLRKAMVVARHENNPILIY"
BASE COUNT          613 a          625 c          776 g          713 t
ORIGIN      
        1 gcttacttca agcagggaaa atacgcgcca gccattcgct acttccagga ctgcattgat
       61 ctggatccgg attttccgga atcgcatttc aatctggcca tactctacat gaagcttggc
      121 cagccaggtc ctgcgcgcgc ttactttgaa gcgtatctgg atttagatcc tgattcagag
      181 tggggagagc tcgcgcgtca atttctggaa gacattcaat ctgcagcaga ggaatagttt
      241 catcttttta ggaatcgcat tctgtgatga ggttttgtct ctgccggtat ttcatctttc
      301 tttaagacgc tgatagagcc gtccagttcc agcactgcca gatacacatc gctgatcgcc
      361 gccacaccat gttcgcgcag tgcctgttcc aattccagaa tcgacacttt ttcctttttt
      421 agattttctg aaacaacggt accccggtga atcaggatgg ctgcttttcc ttctacgatg
      481 ctccgaaaac tcttggagcg agaggcaaga aaagtaagca gaaaattgag tgcgagcagg
      541 gttcccacag ctgcgaggcc gccaggcaca gactgatctg cacccaccat tgcattttga
      601 actgcattgg aaatcagaag gagcaatacc agatcgttcg gggctatctg cccgatttct
      661 tttttaccaa agatgcgcac gaagatcatg agtgcggcaa agacaatgcc tgtgcgaagg
      721 atgatttcta gaatagaact ttgcagtgtg aagagagtca tgataagatc aagcttgccg
      781 ccgatccgtt atgcggcata gcaatttgcg gcgtccgggt ggaggttgcc gagccggcat
      841 agatccatag gggaggcgta tggttctcga gggatcgatt cgctgcgcga atcgtttatg
      901 aaatgcgccg cttcttaaat ttgaaagcct cgaaggcctc tcgcggtcta aaagacggct
      961 ttgcgcctgc gcgctgtctc cgagcgtgca tatgagattc aagcactcga gatcatccgg
     1021 actcaagggg acatggagag ccagctgagg tatttgtggg cggagcccat gcgcacgatt
     1081 tttccattgc ggagattggg caagacgtac aagctgagtc cattacatgc agtaggttcc
     1141 gtttgcgggg aggtggcccc ggaaatactg acggtgtcga tagatcgatg gagggcgcgg
     1201 ggatgcggcg aaaccagttc atgattctag cttgtatctt tgctttcgcg agcgcgagaa
     1261 cagccagtct ttatgcgagt cgtgcaaaaa tgtccgatcc ggcacgggat tctctacata
     1321 agtggcgaaa gtacttgtcc gatgtaaaga accagatgga tcatggcggc ctaaacgatc
     1381 cttggcggga tagcagcgcg attgatgcag gtttcgcgct ttcgggctgg ttgtaagttg
     1441 aaagcgaatg tgttcacacg ccttgcggta acatttgcgg cattctgtgc cattgggctt
     1501 ctcgctaatc cgatccgcat ctgggaaccc gccttgatta ttttgctggg cgagcggtca
     1561 aacgtgggaa gattgcagca atggatagca ttgaaaggcg ggatctggcc tttcttggcg
     1621 gggtttctta gcgtggagat acattggctg gaaggttttg cgttcgttgt attggctttg
     1681 ttgcttgctg gtgcatttgc gcggtcgatc aatggttgct tatggattag tgctgcgtgc
     1741 tctatcttgg ccgctgcttg gcccggttat ctgcgccccg ttgtatttgg cggcattcat
     1801 cgatttgatg aaatatcgac gtacattacg atcttcctga ggatggaatc cggtttcttc
     1861 cttcttctga tattgttcct tcacgcgctt cattcgcggc ggcgcgggag agtatcgcac
     1921 tttgacagtt agcctttgga ctggatcccc gggcgctgca gcagattcta tttgcgcgga
     1981 ggccgcacga gatgatcacg gagtactatc cccaaacatt ggcggcctca cagtgtcgtc
     2041 taacggtaac gtgaacggaa caatcgggac ggatacaccg tatggtgcag tgggcgtgag
     2101 catcaccaat ggccagctgg gagcatctct atccggggat cttgggaact tcaattatgc
     2161 gaatggacag atcggcggga atgtaaacgc aggaccgttt ggtctaggta tccatgatgg
     2221 ccagctaacg cagtttctgg agtattgata tgggcttctc ggcatatgta aacaagtctg
     2281 cggaaagcat gggggaatgg ctcaaactgg gagcctttga acaggatgca aatagcggcg
     2341 agtggtaccc cttggatgaa aggctgatca aacaacttcc ttcccgatat tggaatgcct
     2401 atgaggggta tattggcaat ggttccggtc gcgctgaact tcttgacgat ctcgccaaag
     2461 tatcggatac tttcccgacg atcgagcggt tacttaagtg tgatactgtg attcgccctg
     2521 atcagctgga tgccttttcg cgggaagtgg agcggttgtt aagcgatcgt ataacgcgac
     2581 acctgcgcct catagcgctc cggttgagaa aggctatggt tgtggcgcgc catgagaaca
     2641 atcctatttt gatttactag cgagtggcgg gggaatgaga agattcacga cgcattgtgt
     2701 tcgctcgcgc gaatcgttta aggaaat
//
LOCUS       DAOQJL010000530         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26102_length_2725_cov_4.434457, whole genome shotgun sequence.
ACCESSION   DAOQJL010000530 DAOQJL010000000
VERSION     DAOQJL010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26102_length_2725_cov_4.434457"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1650
                     /gene="rfbH"
                     /locus_tag="PLG78_17475"
     CDS             70..1650
                     /gene="rfbH"
                     /locus_tag="PLG78_17475"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNE24967.1"
                     /translation="MQIIAGNVYEIKLHDSPQSAVALEDGTKDSMVDFAVLFHTTPAT
                     DHVTWHEEGPFQGSHLAAAEVYRLPASSAISKNATAPDSLLADFFRKRTALAVKGNYR
                     FGHKKVPFEEGRPISYAGRVYDEKEMLNLADSALDFWLTAGRFSAKFESSLARYVGAR
                     KLLIANSGSSANLLAFSALTSSRLGDKRLVPGDEVISVAAGFPSTITPIIQNGCVPVF
                     LDVSLPTYNVDVKYLEEAVSPRTRAVMLAHTLGNPFDLAAIKAFCAKHNLWLIEDNCD
                     ALGTEYFIDGEWKKTGSIGDMGTFSFYPPHHMTMGEGGAVCTSRVLLEKIILSFRDWG
                     RDCWCDPGKDNTCGIRFEHKLGQLPQGYDHKYIYSHFGYNLKATDMQAAVGCAQLEKL
                     PSFVEARIKNFNLLYEGLKDLEDVLILPQATANSKPSWFGFLLTVRKGARFTRNQIVA
                     HLESKKIQTRMLFAGNFLRHPSFDEMRATGKGYRVIGDLSVTDQIMNDTFWVGVYPGR
                     TQSEIDFMIREIRNFCRK"
     gene            1652..2206
                     /gene="rfbC"
                     /locus_tag="PLG78_17480"
     CDS             1652..2206
                     /gene="rfbC"
                     /locus_tag="PLG78_17480"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE24968.1"
                     /translation="MFTLRETGLPGCFEVLPRQISDVRGRFIKTFHAPSYSQLGLETE
                     FVEEYYTTSHRGVLRGLHFQIPPADHAKLVYCVAGSVFDAVLDLRKDSPTYGRFAHLE
                     LSEEKRNLLYVPRGIAHGFTTISDECTMIYKVTSVHSPAEDAGIRWDSAGIPWPGSNF
                     IISERDQSFPGLSQYNSPFRMKQV"
     gene            2206..>2725
                     /locus_tag="PLG78_17485"
     CDS             2206..>2725
                     /locus_tag="PLG78_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE24969.1"
                     /translation="MMSSLQGRTILLTGAGGFIGRRLKERLQKAGADVHCLIRAGTQA
                     FSARDHLLSGEGAAAVSNLQKICEDTKPDIVIHLASLFLASHTPADIDSLIDSNIRLG
                     TRLLEAMEKAGIRRIINTGTFWQHFENRPYDPVNLYAATKQAFEDILTYYVNSNAFQA
                     ITLQLYDTYGPGD"
BASE COUNT          706 a          721 c          663 g          635 t
ORIGIN      
        1 tgttcgcgca atcactctgg aacaaatcag agaatacgca agcattcgtg cagccggttg
       61 aggattgaaa tgcaaataat agctgggaat gtatacgaaa tcaaactgca cgactcgcct
      121 caatcggcag tggcactcga agatggaaca aaggattcca tggtggattt cgctgtgctt
      181 ttccacacta ctccagccac agatcatgtg acctggcacg aagaaggtcc tttccagggc
      241 agccacctgg cggcagccga agtctatcgc ctgccagcct cctctgccat ttcaaaaaac
      301 gcaaccgcac ccgattcgct cctcgcagat tttttccgca aacgcactgc tcttgcagtc
      361 aaaggcaatt atcggtttgg tcacaagaaa gtccccttcg aagaaggaag acccatctcg
      421 tacgccggac gagtctatga tgaaaaggaa atgctcaacc tggcagattc tgccctggac
      481 ttctggctga ctgcaggacg attcagtgcg aaattcgaat caagcctggc gcgatacgtg
      541 ggtgcgcgaa agcttctcat cgcgaattca ggatcatccg cgaacttact cgcattttct
      601 gcgctaactt cgagcaggct cggggacaaa agactggtac ccggagatga agtcatcagt
      661 gttgctgcag gctttccatc cacaattacg ccgatcatcc agaacggatg tgtccccgtt
      721 tttctcgatg tgtctctccc cacatacaat gtagatgtaa aatatctgga agaagcagtt
      781 tctccgcgca ctcgagcagt catgctcgca cataccctcg gaaatccatt tgatctggcg
      841 gcaatcaaag cgttctgcgc gaagcacaac ctctggctca tcgaagacaa ctgtgacgct
      901 ctgggtacag agtacttcat agacggagaa tggaagaaaa ctggatccat cggagatatg
      961 ggaacattta gtttttatcc tccacatcac atgaccatgg gcgaaggcgg cgcagtgtgc
     1021 acttcgcgcg tactcctgga aaaaatcata ctctccttta gagactgggg tagagattgc
     1081 tggtgcgatc caggaaaaga caacacgtgc ggaattcgat tcgagcacaa gctcggtcag
     1141 ttaccacaag gatatgatca caaatacatc tactcccact ttggctacaa cctcaaggca
     1201 acggacatgc aggctgcagt gggctgcgcg cagctcgaaa agcttccttc ttttgtagaa
     1261 gcgcgcataa agaatttcaa tctactctat gagggtctca aagacctgga agatgtgttg
     1321 attctaccgc aagcaacggc caactcaaag ccaagctggt ttggattcct gctcacggtt
     1381 cgcaaaggtg cacgctttac gcgcaaccag attgtcgcgc atctggaatc aaagaagatc
     1441 cagaccagaa tgctgtttgc tggcaatttc cttcgccacc cgagcttcga tgaaatgcgc
     1501 gccacgggca aaggataccg cgtcatcggg gacctgagcg tcacagatca gatcatgaac
     1561 gatacattct gggtcggtgt ttatccaggg cgtacgcaga gtgagattga cttcatgatc
     1621 cgcgagattc gaaatttttg tcggaaatga tatgtttact ctgcgagaga caggtctacc
     1681 cggctgcttc gaagtactgc cgcggcagat ttcggatgta agaggacggt tcatcaagac
     1741 attccacgca ccatcctact cgcaactggg attggaaaca gagttcgtcg aagaatacta
     1801 tacgacctcg catcgcggtg ttcttcgcgg cctgcatttt caaattccgc ccgcagatca
     1861 tgcgaagctc gtttattgcg tcgctggatc cgtatttgat gccgtgctcg accttcgcaa
     1921 agactcaccc acgtatggca ggttcgctca ccttgaactt tcggaagaga aacgcaacct
     1981 attgtatgtt ccgcgcggaa tcgcacacgg ctttactacg atttctgacg aatgcacgat
     2041 gatctacaaa gtaacttcgg ttcattcgcc tgcagaggac gcaggcattc gctgggatag
     2101 cgctggaatc ccctggcctg gcagcaactt catcatctcg gaacgagacc aatctttccc
     2161 gggtctatct caatacaata gcccattccg gatgaaacaa gtctgatgat gtcaagcttg
     2221 caaggtagga ccattcttct caccggggcg ggtggattca ttggaaggcg gttgaaagag
     2281 cgcctccaaa aagcgggggc ggatgtccat tgtctgattc gggctggtac tcaggctttc
     2341 tctgcccgag accatttgct gagcggcgaa ggtgcagccg cagtctcgaa cctgcaaaag
     2401 atttgcgaag acacaaaacc ggatattgtc atccatctgg cgtctctctt tctcgcctca
     2461 cacactcctg cagacataga ctccctcata gacagcaaca ttcgcctggg tacccgactt
     2521 ctggaagcca tggagaaagc gggaatccgt cgtatcatca ataccggaac cttctggcag
     2581 cattttgaaa atcggcctta cgatccggtc aatctgtacg cagccaccaa acaggcattt
     2641 gaagacattc ttacgtacta cgtaaattca aatgcgttcc aggcaatcac gcttcaactc
     2701 tatgatactt atggtccagg agaca
//
LOCUS       DAOQJL010000531         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26274_length_2715_cov_4.550376, whole genome shotgun sequence.
ACCESSION   DAOQJL010000531 DAOQJL010000000
VERSION     DAOQJL010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26274_length_2715_cov_4.550376"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PLG78_17490"
     CDS             <1..1289
                     /locus_tag="PLG78_17490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE24970.1"
                     /translation="DLQTAVEYLNMANRELEKKILVNKRELDMARSIQKGFVPQRVPD
                     WNGLQFWVKFFPFSEVSGDFYDYFYLGSTRFGMVAGDVSGHGVPAALISAIAKFSFSN
                     NRLDSPAQVLESVNMDMLNFVRGEGYLTGFYMIVDNNYGITYSVAAFPNPILFRAKTR
                     HIEQLPGHGTLLGMFPDAGRSYMDYQTYLEPGDKLFIFSDGLIEATNVQGESLGEDKL
                     IDTILETEGMDVQRSSEHIMGMYNRFIRGTDQKDDLTLITAMLSERLSEFDETVMHAR
                     RAFHSGDVTHACTLLKKAISIFPRQTAALFLLGKYLALEGKYEEALEYLRQYNGLKPY
                     NADSYRIKGYCYYRLGNLEAASEQFKRALGLRWENPSVLYNQTMVLLKLNQVEEARKL
                     FEYFEHTNPGHFLLGKIRDKLRAMNQHTEDDAPGAD"
     gene            complement(1324..2058)
                     /locus_tag="PLG78_17495"
     CDS             complement(1324..2058)
                     /locus_tag="PLG78_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE24971.1"
                     /translation="MVCIPGAKFIRGSNLPSKDEDTGKMVTDEAPEEVVEVSTIFMDI
                     YEVTFSDFQKCIAAGKCRAAHPNYTGYSNPRMPMLGVNWFDSRDYCAFVGKRLPTEAE
                     WEHAARGDAGEIFPWGNEPATCARAIIQENGKKGCGPGTTVDVGSRPAYRYGLYDMAG
                     NSWEWVNDWYSPGYAKCGDDCRGKDPKGPCKGADVCPGFPEKIVKGGSWWWDAEYARS
                     SNRRPHFPANKPFHHFGFRCAASAER"
     gene            2202..2576
                     /locus_tag="PLG78_17500"
     CDS             2202..2576
                     /locus_tag="PLG78_17500"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HNE24972.1"
                     /translation="MSRVRSWAIFTIRGSIVPELITRDLGIEPDRVYDDGRDRIWQIN
                     STLGATETTEEHFHELLLRLLPKRLTLRKLAEDHRLEFYCFLEKPQEMSLPITLSPRI
                     LLLCGFLHAHIDVEVKDLDEEA"
BASE COUNT          717 a          700 c          642 g          656 t
ORIGIN      
        1 gtgacttaca gactgcagta gaatatctaa acatggccaa ccgggaactg gagaaaaaga
       61 tcctggtcaa caagcgcgaa ctggacatgg ctcgaagcat ccagaaaggt tttgttccgc
      121 agagagttcc tgactggaac ggtcttcagt tctgggttaa gttttttcct ttttcggaag
      181 tttccggcga cttctacgac tatttctacc tggggtccac acgctttgga atggtggctg
      241 gagatgtttc cggacatgga gtgccggccg ctctcatcag cgcgattgca aagttctctt
      301 tttcaaacaa tcgcctggat tcgccagcac aggtactaga aagcgtgaac atggatatgc
      361 taaacttcgt aaggggggaa ggctatctca ctggttttta tatgatcgtg gacaacaact
      421 acgggatcac gtattctgtg gccgccttcc ccaacccaat tctatttcgc gcgaagacgc
      481 gacacattga acaattaccc ggtcacggaa ctcttcttgg aatgtttccg gatgcaggca
      541 gatcttacat ggactaccag acgtacctgg aaccgggaga taagcttttc attttttcag
      601 atggtctcat tgaagcaacg aatgtgcagg gagagagcct gggagaagac aaactcatag
      661 atacaattct ggaaacagaa gggatggatg tgcaaagaag ttctgaacac atcatgggaa
      721 tgtacaatcg gttcatacgc ggtacagacc agaaagatga cctgactctc atcactgcca
      781 tgctcagcga aaggctgagc gagtttgatg aaacagtcat gcatgcgcga agagcgtttc
      841 attcaggaga cgttacgcac gcctgcactc ttctcaaaaa agcaatttcc attttcccaa
      901 gacaaacagc cgctctcttc ttgctcggga agtatctggc gcttgaaggc aaatacgaag
      961 aagctcttga gtatctcagg caatacaacg gccttaaacc ctacaacgct gattcttaca
     1021 ggatcaaagg atattgctac tacagactgg gcaacctgga agctgcgtcc gagcaattca
     1081 aacgggctct gggtctacga tgggaaaacc catccgtttt gtacaatcag accatggttt
     1141 tgttgaagct caatcaggtg gaagaagccc gcaaactatt tgaatacttt gagcatacca
     1201 acccgggcca tttccttctg ggtaaaatca gagacaagtt gcgagcgatg aatcagcaca
     1261 ccgaagacga tgcgcccggt gcggattaac ttctagaata ttttcaagct tattcaagat
     1321 cgtctaacgt tctgcagagg cagcgcatcg aaatccaaag tgatgaaacg gcttgttagc
     1381 aggaaagtga ggtctccgat tggagctacg cgcgtattcc gcatcccacc accaggatcc
     1441 tcccttcaca atcttctccg gaaatccagg acacacgtct gcgcccttgc acggaccttt
     1501 tggatctttt ccacggcaat cgtctccaca cttcgcataa ccgggtgaat accagtcatt
     1561 gacccattcc cacgaattgc cggccatgtc gtagagtccg tagcgataag cgggtcttga
     1621 tcccacatcc accgtggtgc caggcccgca tcctttcttt ccattttctt gaatgatcgc
     1681 tcgcgcgcat gttgccggct cgtttcccca tggaaaaatt tctccagcat ctcctcgcgc
     1741 tgcatgttcc cactctgctt ccgtgggtag acgctttcct acaaaagcgc aatagtcgcg
     1801 cgaatcaaac cagttcactc cgagcatggg catcctggga ttgctatagc ctgtgtaatt
     1861 tggatgggcc gcacgacatt taccggccgc aatgcatttc tgaaaatcgg aaaaagtgac
     1921 ttcatagatg tccatgaaaa tggtgctcac ctccactact tcttcaggcg cttcgtcggt
     1981 gaccatcttg cctgtgtctt catctttgga aggtagattc gaaccgcgta tgaacttcgc
     2041 acccggaatg cagaccattc ccgccggagg atgcagagca gcgcagggat cctgtttaaa
     2101 cgcggcagga gtgcagccaa atagaatcaa aatcatacat gtaaaaaggc gcatcatgct
     2161 aaaatgcttg tgaagtttga gtgtaaacaa ataaccgaac cgtgtcgcga gtcagaagct
     2221 gggcgatctt tacgatccgt gggagcatcg tgccggaact catcacgcgc gatctgggaa
     2281 tagaaccaga tcgtgtttat gacgacggca gagatagaat ctggcaaatc aactccactc
     2341 tgggtgccac ggaaacaacg gaggagcact tccacgaact actcctgcgg ctgctgccaa
     2401 aaagattaac gctgcgaaaa ctcgcagaag atcaccgact agaattctat tgcttccttg
     2461 aaaaacccca ggaaatgagt ctgcccatca ccctctcccc cagaattctg ctcttgtgcg
     2521 gattcctcca cgctcatatt gatgtggagg taaaggacct ggacgaagag gcctaacgtt
     2581 attcgatctt gccgtacttc ttataaagaa gtgcgatggc gtcatctgac tcaatcccgc
     2641 gcatatccag tacaaagtcg gctgctttct cataccaggg cagccttctc tctaacaatt
     2701 ccctgtaacc acctg
//
LOCUS       DAOQJL010000532         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26283_length_2714_cov_5.606995, whole genome shotgun sequence.
ACCESSION   DAOQJL010000532 DAOQJL010000000
VERSION     DAOQJL010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26283_length_2714_cov_5.606995"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLG78_17505"
     CDS             complement(<1..795)
                     /locus_tag="PLG78_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNE24973.1"
                     /translation="MPWWAGSMIQTLSVEILRFRQSFLGQIRAFFDSRGYLEVQTPAL
                     NPTGAVEPFIDSFQVHATGDRKSPAGPSGGTEGYLITSPEYNLKTVLAASPVPIYEIC
                     HSFRAGDVGAEHTEEFLMLEWYRPGATESDLILETNSLLVHLSRSFPLPTGLRLPEKP
                     RTLAVGDLFAQAGIGGLGRDVLERAAVDMKLGSKADRYDELFFSLFLNRIEPSLRIPE
                     PVYLNGYPEELAALSNTERGLAKRFELYWNGLELANGYEELRNKEEH"
     gene            937..1857
                     /locus_tag="PLG78_17510"
     CDS             937..1857
                     /locus_tag="PLG78_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNE24974.1"
                     /translation="MSAIHDTNKIQKEGREPRVVREKRKSGRDSEHGDSILNWYLNEI
                     HKIPMLDRETEFEVANLAKSGDRAARDRLVQANLRFVVTVARKYQSYGLTMMDLINEG
                     NMGLIRAAERFNPDRGYHFISYAVWWIKQAILFAIQQKAALIRLPLNRTADLRRIDEA
                     EKWIENHDDVENNLETLSGMLDMKSQEIQHLVNMSRDYLSLDAPMGDGEDFTLGDNIM
                     DQTEQVPESRVEGEELKREIEESLESLTSREKKVLQMRFGLQGSAVFSLQKIGKKVGL
                     SKERIRQIEKKAIRKIRTGTSGQKLLAYVN"
     gene            1877..>2714
                     /locus_tag="PLG78_17515"
     CDS             1877..>2714
                     /locus_tag="PLG78_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24975.1"
                     /translation="MRALQFTLAFLLILGPLLFRFPAREESFSPSINALYYYPFEGDP
                     SPLPNSWVKESVRPFNAVWIPNGDASRLILPNRRVDLNSEKMAFAPIHGDGYFIFQRV
                     GSEISFLSSLGELLWKKPFPAYPVTAPSGSPVFLMTGDANRVDVLDASGNLKGVKHIA
                     GNFLTDYKFSRSGRTCFVFGAGGLIVIDPQGEIAFKFESQDPAFYKSCALSPDGQVAA
                     VHALRGESDVVFVVRQKEKKASVSETLPLSAEYTHSLPLAVSNSGSVLVIPPEGALFF
                     KSG"
BASE COUNT          678 a          676 c          715 g          645 t
ORIGIN      
        1 gtgctcttct ttgtttctca attcttcgta cccgttggcc agttcgagcc cattccagta
       61 gagttcgaat ctctttgcca gaccccgttc cgtattgctg agagctgcga gttcttccgg
      121 atatccgttt agatagacag gttctggaat tcgaagcgat ggctcgatac gattgagaaa
      181 caaagaaaag aagagttcgt cgtatcgatc cgcctttgat cccagtttca tgtccacggc
      241 ggctctctcg aggacatccc gccccaggcc tccgattccc gcctgggcaa acaaatcgcc
      301 tacggcgaga gtccggggtt tttctggaag tcgaagaccg gtcggaaggg gaaaggatcg
      361 agaaagatgg acgagcaggg agtttgtctc cagaatcagg tctgattctg tcgcacctgg
      421 cctgtaccat tccagcatga ggaattcttc cgtatgctct gcacccacat ctccagcccg
      481 aaaagagtga cagatctcgt agattggcac gggacttgcc gccaggaccg ttttgaggtt
      541 gtattccggg ctcgtgatca ggtagccttc tgtgcccccc gatgggccgg cggggctttt
      601 tcgatcgccc gtagcgtgca cctggaagct gtcgatgaat ggttccacgg ctccggtggg
      661 gttcagtgcc ggggtttgga cctccagata gcctctggaa tcaaaaaatg cccggatttg
      721 gccgaggaag gactgcctga atcgtagaat ttcaacggaa agagtctgaa tcatggaacc
      781 tgcccaccag ggcatgggtc taagaggcaa ttcccgatca gggagtcaaa aaaaagcgaa
      841 gtttaaacga ttaaaatggt tgatcagggc gagtagatgc cgtattttag aatacttcta
      901 aatctgaagg gcttttgatc ttgaggtgag gacagaatga gcgcaataca cgatacaaat
      961 aagatacaaa aagagggccg tgaacccagg gtcgttcgag aaaaacgcaa atccgggcgc
     1021 gactcagagc atggggattc catcctcaac tggtacctga acgaaatcca caagattccg
     1081 atgctggacc gggagactga gttcgaagtg gccaacctcg caaaaagcgg agacagagca
     1141 gccagagaca ggctggtcca ggcaaacctt cgctttgttg tgactgtagc ccgcaaatac
     1201 cagtcttatg gtctcaccat gatggacctc atcaacgaag ggaacatggg cctcattcgc
     1261 gcagcggaga ggttcaaccc ggaccgcggc tatcatttca tctcgtatgc cgtatggtgg
     1321 atcaaacagg ccattctgtt tgccatccag caaaaggcag cgttgattcg ccttcctctg
     1381 aatcgcacag cggaccttcg cagaatcgat gaagcggaaa agtggatcga aaaccacgac
     1441 gacgtggaga acaatctgga aactctttct ggaatgctcg acatgaaatc gcaggagatc
     1501 cagcacctgg tgaacatgag ccgtgattat ctgagcctgg atgctcctat gggagacggc
     1561 gaagatttca ctctcggcga taacatcatg gatcagacag agcaggtccc tgagagccgc
     1621 gtggaaggcg aagaactgaa gcgcgaaatt gaagagagcc tcgagagtct gacttctcgc
     1681 gagaagaaag tactgcagat gagatttggt ctgcagggaa gtgcagtatt ctctctgcag
     1741 aagattggca aaaaggtcgg gctgtccaaa gagcgcattc gccagattga gaaaaaagca
     1801 atccgcaaaa ttcgcactgg aaccagcggc caaaaactac ttgcttacgt gaattgaaac
     1861 agaagagctc tcttcagtga gagctcttca atttacactc gcattcctac tgattctcgg
     1921 gccccttttg tttcgctttc cggcccggga agagtctttt tccccatcca tcaacgcact
     1981 ctactactat ccgttcgaag gagatccgtc tcctctccct aattcctggg tcaaagaatc
     2041 cgttcgacct ttcaatgcgg tctggattcc gaatggagat gcatccaggc tcattcttcc
     2101 caatcgtcgc gtggatttga attccgagaa gatggcattt gctcccatcc acggagacgg
     2161 ctattttatt tttcagagag taggatctga gatcagcttc ctgagttccc tcggagaact
     2221 tctatggaag aaaccatttc ctgcgtatcc tgtgactgct ccctcaggtt cgcctgtatt
     2281 cctgatgacc ggagatgcaa accgggtaga cgttctcgac gcgagcggca acctcaaagg
     2341 agtcaaacac atagccggga actttctaac cgattacaaa ttctccagat caggtaggac
     2401 ctgctttgta tttggcgccg gaggattgat cgtaattgat ccgcagggag aaattgcgtt
     2461 caagtttgaa tcccaggatc cagcgttcta caaaagctgc gccctttctc cagatggaca
     2521 ggtggctgcg gtgcatgctt tgcgcgggga atcagatgtt gtatttgtgg tgcgacagaa
     2581 ggaaaaaaag gcctccgttt cagagactct tcctctttcc gcagagtaca ctcattcact
     2641 tcctcttgcg gtctccaatt ctggatcggt tctggtgatt cctcctgaag gagctctgtt
     2701 tttcaagtca gggg
//
LOCUS       DAOQJL010000533         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26319_length_2712_cov_4.728265, whole genome shotgun sequence.
ACCESSION   DAOQJL010000533 DAOQJL010000000
VERSION     DAOQJL010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26319_length_2712_cov_4.728265"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2138
                     /locus_tag="PLG78_17520"
     CDS             84..2138
                     /locus_tag="PLG78_17520"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNE24976.1"
                     /translation="MAAKKKPGKAAKKTGKKSAKKTGAKLSAQQPAPRPRGRPPGTKS
                     EKKRGRPPGSEKQASEARDVPRKKRDQQPFVTLKKMVQEAITSALPEYLKESRIPIEY
                     SREEKFGDYAATAAMDKDFRETLSKRNPACKNPREAGAYLLKQLRASEESSTIFANVE
                     IAGPGFLNISISPSVLATFAARAVKNPETYGRTESAEKRKIIFEFVSANPTGPLNIVS
                     ARAAALGDSCCNLLSWAGESVFREYYVNDYGNQIQLLGQSCFLRALEETGIPVKISEK
                     TDHGPVYPEGPGLPFPSEGYHGDIIKDIVQEIRKRKSVLIGPRVVEHARQIAGSPDVP
                     LDFISRHPAMQELSDKLGEAAVSVFLDTQQRDLERFRVKFDAFYRESSLHKQNKVLSA
                     RNFLAEYVRTEDGKQIFESKKFGDDLDRVIIREDGRPTYLLADIAYHKTKMDRGFDEI
                     INIWGPDHHGYIKRLAGALVAMGYSAEKFKVLLAQQVNLLEHGQPVVMSKRAGKFVTM
                     DSLMNEIPIDVIRYFFVMRSFEAHLDFDFAEAADTSEKNPYYYVAYAHARICSIQRKA
                     LEKGISIPVEAREMEECMRTLEWTADRRRLLYLVARFPEEVQDAARVLEPHRLINFLY
                     HLANALSRFYAPKENRVIDQDERTARGLLVLLSAVATCLKNGLAILGMTAPDRMTREE
                     SA"
     gene            complement(2135..>2712)
                     /locus_tag="PLG78_17525"
     CDS             complement(2135..>2712)
                     /locus_tag="PLG78_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE24977.1"
                     /translation="NAAGPIHVLINNAGVFMKEKKLTVDGFETTFAVNHLAVFLLTNE
                     LLPSMPDGSRIITLSSIAHKNGKIDFDNLQSEKSFDGYAAYAMSKLCNVVFSYELARR
                     GASRGILSNAVHPGVIQTKLLKEGFNMDSAGSLEEGAATSIYLASSPEVQNVTGKYFV
                     SSRESVSTAASRDEALQRKLWDVSEQLLSRF"
BASE COUNT          751 a          690 c          687 g          584 t
ORIGIN      
        1 ggaggcgctt gtgaccacca aaaaagtgct ggttgccaga ctggccgatc tcgatccttt
       61 cctgaaaact caggcagagg gccatggcag caaaaaagaa gcccggtaag gcagcgaaaa
      121 agacgggcaa gaaatcggcc aagaaaaccg gcgccaaact ttccgcacaa caaccggccc
      181 ctcgtcctcg cggcagacca ccgggcacca aatcagagaa gaaaagaggg agacctcccg
      241 gatcagagaa gcaagcctcg gaagcgcgag acgttccgcg aaaaaagcgc gatcagcaac
      301 cattcgtcac tctgaagaaa atggtgcagg aagccatcac ctcggctctc cccgagtatc
      361 tgaaagaatc tcgcatcccc attgaatatt cccgcgaaga aaagttcgga gactacgccg
      421 ccacagccgc gatggacaaa gacttccgcg aaacattgag taaacgcaat cctgcgtgca
      481 agaatccgcg cgaagccggt gcgtatttac tcaaacagtt acgagccagt gaagaaagtt
      541 ctaccatctt cgcgaatgta gagatcgccg gaccgggttt tctaaatatt agcatctccc
      601 cttccgtcct cgctacgttt gccgcgcgag cagtgaaaaa tccggaaacg tacggcagaa
      661 cagaatctgc ggaaaaaaga aagatcattt ttgaattcgt ttctgcaaat ccgaccgggc
      721 cactgaatat agtttcagca agagccgcag ccctgggtga ttcctgttgc aatctacttt
      781 cctgggcggg ggaatctgtc tttcgcgaat actatgtaaa cgactacgga aatcaaattc
      841 aacttcttgg ccagagctgt tttcttcgcg ctctcgaaga gacaggaatc ccggtaaaaa
      901 tttcagaaaa aacagatcac ggacctgtat atccggaagg tcctggactg cctttcccat
      961 cagaagggta tcacggcgac atcatcaagg acattgtcca ggaaattcgc aaacgaaaat
     1021 cggtgttgat cgggccgcgc gtagtagaac atgcgcgaca gatcgccggg tcaccggatg
     1081 ttccacttga ttttatttcc aggcatccgg ccatgcagga gctttcggac aaactcgggg
     1141 aagcagccgt cagtgtattt ctggatactc aacagagaga tctggaacgc tttcgcgtaa
     1201 aattcgacgc cttctaccgc gagtccagtc ttcataaaca aaataaagtt ctttctgcgc
     1261 gcaattttct ggcggaatac gttcgcacag aagatggcaa gcagatcttt gaaagcaaga
     1321 agtttggaga cgatctggat cgagtgatca ttcgcgagga tggccggcca acgtacttgc
     1381 ttgcggacat cgcatatcac aaaacaaaaa tggaccgtgg ctttgatgaa atcatcaaca
     1441 tctgggggcc cgatcatcac gggtacatca aacgtctcgc cggcgctctt gtagccatgg
     1501 gatactccgc agaaaaattc aaggtacttc tggcgcaaca ggtaaattta ctggagcacg
     1561 gccagccagt tgtcatgagc aagagagcgg gtaagtttgt gaccatggac agcctcatga
     1621 atgaaatccc aatcgacgtc attcgctact tcttcgtgat gagatctttt gaggctcatc
     1681 tggattttga tttcgcagag gccgcggaca cttctgaaaa gaatccttat tattacgtag
     1741 catacgcgca cgctcggatc tgttctatcc agagaaaagc cctggaaaaa gggatctcca
     1801 ttccggtgga agctcgcgag atggaagaat gcatgcggac tcttgaatgg acagcagaca
     1861 gaagaagact actctatctg gttgccaggt tcccggaaga agtgcaagac gcagccagag
     1921 tacttgaacc gcacagactc ataaactttc tttatcatct ggccaatgct ctttctcgat
     1981 tctatgcacc caaagaaaac agggtcattg atcaagatga gcgcacagcc agaggactgc
     2041 ttgtacttct gagtgcagtg gccacctgcc tcaagaatgg cctggccatt ctgggcatga
     2101 cggcaccgga tcgcatgacc cgcgaagaat cagcctagaa gcgcgaaagc aattgctctg
     2161 aaacatccca gagtttgcgc tgcaatgcct catcgcgaga ggcggcagta cttacgcttt
     2221 cgcgcgagct tacgaagtat tttccagtca cattctgaac ttcaggagaa ctggcgagat
     2281 aaatcgatgt agccgcaccc tcttccagag acccggcaga gtccatgttg aaaccttctt
     2341 tgaggagttt ggtctgaatg actcctggat gcactgcgtt agacaggatg ccacgcgaag
     2401 cgccgcgacg agcgagctcg taggaaaaaa ccacattgca cagcttgctc atcgcgtatg
     2461 cggcgtaacc atcgaacgat ttctcagact gcagattatc aaaatcgatt ttgccattct
     2521 tgtgggcgat ggagctgaga gtaatgatgc gcgatccatc cggcatggaa ggaagaagct
     2581 cattcgtgag caggaacaca gccagatgat tgaccgcgaa cgtagtttca aatccatcca
     2641 cagtaagctt tttttccttc ataaagacgc ccgcgttgtt gatgagcacg tggatgggcc
     2701 cggccgcatt gg
//
LOCUS       DAOQJL010000534         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26452_length_2705_cov_4.448679, whole genome shotgun sequence.
ACCESSION   DAOQJL010000534 DAOQJL010000000
VERSION     DAOQJL010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26452_length_2705_cov_4.448679"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PLG78_17530"
     CDS             complement(<1..1212)
                     /locus_tag="PLG78_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNE24978.1"
                     /translation="METTAVKRQLEIKETDSVVIRFSGDSGDGMQLTGMQFTDTTAIL
                     GNDLSTFPEFPAEIRAPAGTLAGVSGFQVHFGSKEIFTPGDKYDVLVAMNAAALKADL
                     KNLREHGVIIVNTAGFDERNLKLAQYPEGAHPLDDGSLDHYRLYKLDISRLTKDSMDG
                     LEVSAKDVDRAKNMFVLGLLFWMFERSVSNTEKFIQEKFSKKPLIQDANIRALRAGFN
                     YGETAEIFTTRYHVAKAKMPPGVYRNITGNQALAIGLIAAGEKSGLPIFLGSYPITPA
                     SDILHELSRYKANGVRTFQAEDEISAITSAIGASYGGALAVTTTAGPGLALKTEAMGF
                     ATMLEIPLVIIDVQRGGPSTGLPTRTEQADLMMAMFGRHGEAPLPIVAVATPADCFET
                     IYEACRITLEHM"
     gene            1365..2021
                     /locus_tag="PLG78_17535"
     CDS             1365..2021
                     /locus_tag="PLG78_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24979.1"
                     /translation="MKLDRYVVIKENSGGHNAVAFCDVDSYVYLNPSASSSCTIEEIS
                     HEIASEEGEPGESVRARIAACNPGLDVAKAAFQAGSLIRVKTEQTPSSQTVLEDIPMA
                     GGKYLKFLKWAIGGIKWAAEKAEGGPTSDAKSRWEATLASRRQQILDQHRAILSALQI
                     PLGENSFERVINKVIPDFLRKRGISSNLLQLMFSRDGYLVFRFQHIRIVTSVPVDAYS
                     "
     gene            2042..>2705
                     /locus_tag="PLG78_17540"
     CDS             2042..>2705
                     /locus_tag="PLG78_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24980.1"
                     /translation="MMLFELRARHVFAALAIMGALQSTRAEPLTASFPRLGRVTASSG
                     LTLRTSPKASAPSITVIPNGANVFVVRPTSQMDTIEGIKASWFFISYGGQSGFVFSGF
                     LTSDLQQRPDFTEEDATIIRRLAQLSGLRVPSLKVRYPMRGNPYVADHFTLKDRRQLG
                     SFIVAIIAPEKTDCQGFFDECFSLIGTTNGEYLFSDVDTFPVGPLYYADSRLVFFMQT
                     EAE"
BASE COUNT          650 a          685 c          688 g          682 t
ORIGIN      
        1 catgtgctcc aacgttatgc ggcatgcttc ataaattgtt tcgaaacagt ctgcaggagt
       61 ggccacagca acgatgggaa gaggagcttc tccgtgtctt ccgaacattg ccatcatcaa
      121 atccgcctgt tctgtgcgag tgggaagacc tgtagacggg cccccgcgct gcacatcaat
      181 gatgaccagc ggaatttcaa gcatggttgc aaaacccatt gcttctgttt ttagagcgag
      241 accaggaccc gcagtggttg taactgcaag cgcaccgcca tacgaagctc cgatggcact
      301 tgtgatggca gaaatttcat cttccgcctg gaacgtgcgc actccattcg ctttgtagcg
      361 agaaagctcg tgtagaatgt ccgatgcggg agtgatggga taggaaccca ggaaaatcgg
      421 cagaccggat ttttctccgg cagcaatcaa gccaatggcc agcgcctgat tcccggtgat
      481 attgcgataa acaccaggag gcatctttgc ttttgcgacg tggtaacgcg ttgtgaaaat
      541 ctctgccgtt tcaccgtagt tgaaaccggc gcgaagtgcg cgaatgttcg cgtcctgaat
      601 caaaggcttc tttgaaaatt tctcttgaat gaatttctca gtatttgaga cggatctttc
      661 gaacatccag aaaagcaaac caagaacgaa catgttcttc gctcggtcca cgtccttcgc
      721 agatacttcc aggccatcca tgctatcctt tgtaaggcgt gatatgtcca gcttgtacag
      781 acgatagtga tccaggctgc cgtcatcgag tggatgagca ccttcggggt attgtgcgag
      841 ctttaggttg cgctcatcaa agcctgcagt gtttacaatg atcactccgt gttcgcgcag
      901 attcttgaga tccgctttga gtgctgcggc gttcatggca acgagcacat cgtatttgtc
      961 gcctggagtg aaaatctctt tgcttccgaa atgaacctga aatccggata cgccggccag
     1021 agtgcccgcc ggtgcgcgaa tttcagccgg aaattccggg aacgtactta agtcgtttcc
     1081 tagaatggct gttgtatctg tgaattgcat tccggtgagc tgcattccgt cgccggaatc
     1141 tcccgaaaat cgaatgacta cgctatctgt ttctttgatt tcaagctgac gcttgactgc
     1201 agttgtttcc attgttggtt tgaattaccg ttgagtccaa ggtccagctc gggcagacca
     1261 gtgtcgacta caaatagggc tataaacctg cgtcaaaacg tcagatccag ccgttttcca
     1321 cgcaaaaaag atgggcagga tcattccgcc tggtaaggga agctatgaag ctcgatcgtt
     1381 acgtggttat caaagaaaac tctggaggac acaacgcagt tgcgttttgt gatgttgatt
     1441 cttatgtcta tttgaatccg tcggcctcct ccagttgcac gatcgaagag atctctcacg
     1501 aaattgcaag tgaggagggc gaaccaggag aaagtgtgcg cgcgagaatt gcagcgtgca
     1561 atccaggtct cgatgtagcg aaggctgcgt ttcaggcagg ctccttgatt cgcgtcaaga
     1621 cggaacaaac tccttccagt caaacagtcc tggaagacat tccgatggcc ggcggcaagt
     1681 atctgaaatt cttgaaatgg gccattggcg gaatcaagtg ggcggctgaa aaagcagaag
     1741 gtggcccaac ctcagatgcg aaaagcagat gggaagcaac gcttgcatct cgtcgtcagc
     1801 aaatcttaga tcaacatcga gccatcctct ctgcactcca gattccgctc ggagagaatt
     1861 catttgaaag ggtgatcaac aaggtgatcc cggatttctt gcgaaaacgc gggatcagct
     1921 cgaatctatt gcaactcatg ttttcgcgcg atggatacct ggtctttcgc tttcaacaca
     1981 tccgaattgt aacgagtgtg ccggtagacg cctactcgta gtcagacgaa ccagagtctc
     2041 catgatgctc tttgaactgc gcgctcgcca tgtgttcgct gctctggcaa tcatgggagc
     2101 gttgcagtct actcgcgccg aaccacttac ggcgtccttt ccacgacttg gccgggtcac
     2161 cgcatcgtct ggcctgacgc ttcgcacatc tccgaaagca agcgctccat ccatcactgt
     2221 gattccaaac ggtgcaaatg ttttcgtggt cagaccaacc agccagatgg atacgatcga
     2281 aggaatcaaa gcctcctggt ttttcatcag ctacggagga caatctggat tcgtcttcag
     2341 cggctttcta acttctgatc tacaacagcg gcctgatttc accgaggagg atgcgactat
     2401 cattcgcaga ctcgcgcaat tgtccggcct tcgcgtgccc tcactcaagg taaggtatcc
     2461 gatgcgcggt aacccttacg tcgcagatca tttcactctg aaagatcgca gacaactggg
     2521 atcattcatt gttgcgatca tcgcgcccga aaagacagac tgccagggtt tttttgacga
     2581 atgcttctct ttgatcggga ctacaaacgg agagtattta ttttcggatg tggacacttt
     2641 ccctgtgggc cctctgtact acgcggattc acggcttgta tttttcatgc aaaccgaagc
     2701 agaag
//
LOCUS       DAOQJL010000535         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26526_length_2701_cov_3.662509, whole genome shotgun sequence.
ACCESSION   DAOQJL010000535 DAOQJL010000000
VERSION     DAOQJL010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26526_length_2701_cov_3.662509"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLG78_17545"
     CDS             complement(<1..204)
                     /locus_tag="PLG78_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002073388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24981.1"
                     /translation="MKSRLLFFLFAISAAGAFAQEVKFSWPMERGFPELRGLTSTFGE
                     SRGDHFHNGVDISSLGEPVHPVAP"
     gene            228..1106
                     /locus_tag="PLG78_17550"
     CDS             228..1106
                     /locus_tag="PLG78_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE24982.1"
                     /translation="MDTPIPSGTEQEAILSLVRRCAGGEDAALREFFETYSRDIYNFP
                     MRVFHLDEDGASDFYLYALEILKNGNRFKSFSGRSTFRTWFYSVLRNLVIDWLRITRF
                     QRKPSQPLLEAADQPVPEEEPLVQDWLSGLPLETRCLVKLVFVYYLEMDAEELAHVCD
                     RSGLNKVKLAERIASIKAGLAEKEIENLTRTDKITSLYVGILDLRSRRARVIEEDTQH
                     VRRHEVERIDHAIAKKTQQREKLLARKEKGHFVVRLPYREAAELLAMPEGSVSVAVMR
                     AFEKLREMQKKLTEGK"
     gene            1158..1742
                     /locus_tag="PLG78_17555"
     CDS             1158..1742
                     /locus_tag="PLG78_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24983.1"
                     /translation="MFDEAGLTPQEILEIEEAFVRQSLVRRGDEEFQALLSEHLMQGE
                     LLPVPQTLLDISGRVLTNSAPEFQIRLHPENVITSVVSAVRGFFSEPLALDPVRSVGL
                     ERRPALDIGKAGSVIRYRIIRESEAEVLLAVRVDAPGALRANLRRDGRIVASQTMAIG
                     ESSLAFEHLGAGNYTLEITGSRAESVSFHINSEV"
     gene            complement(1743..>2701)
                     /locus_tag="PLG78_17560"
     CDS             complement(1743..>2701)
                     /locus_tag="PLG78_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNE24984.1"
                     /translation="QRKSGKYKGLIDFLERVDLRLCNRRTVEALVLSGGFDCFGYTRK
                     ALMGSLDLAYQRAQKSQEDRASGQVSLFGEAMATEDFIPKGEGVSEFEESILLRQERE
                     MLGCYLSGHPLSRFDRVLSRAGISTIENLQRMKANDRVELAGVVGDISKRITKTGKEM
                     WNIRIEDKTGSIEAVLFSSTIEESRNFLQKDTPLIFKGRVEKDDAKPMPRLRVESVKE
                     LTDEAVEEKLEKSLHLQIQTLTSVDGEQVLKQIQTILKCHKGNLNVYFHLPSQQTPGA
                     QTVIRAHNSYSVAMNRAMIDQLKQLACVRSVYVSIGNQVRPV"
BASE COUNT          640 a          653 c          734 g          674 t
ORIGIN      
        1 gggagccact ggatggaccg gttcgcccag actggaaata tccactccgt tgtgaaagtg
       61 atctccgcgt gattcgccga aggtggaggt gaggccgcgg agttctggaa acccgcgttc
      121 catgggccag gagaacttaa cttcctgtgc gaaagcgccg gctgcggaaa ttgcaaaaag
      181 aaaaaatagg agtcgagatt tcatcgcctt gttgtgacgt gggtttgatg gacacgccca
      241 ttccgagcgg aacagagcag gaggcaattc tttctctggt ccgcagatgc gcaggcggtg
      301 aggacgcggc tctgcgtgag ttcttcgaaa cctattccag agatatctac aatttcccaa
      361 tgcgggtgtt ccatctggac gaagacgggg cttccgattt ctatctatac gccctggaaa
      421 tcttgaagaa cggaaacagg ttcaagtcat tttctggaag atccactttc aggacctggt
      481 tctattcagt ccttcgcaat ctggtcattg actggctacg catcaccagg tttcagcgca
      541 agccttctca gcctttgctc gaggcggcgg atcaaccagt cccggaggag gaaccgctgg
      601 ttcaggactg gctgagcgga ttgcctctgg agaccaggtg cctcgtaaaa cttgtatttg
      661 tgtactatct ggaaatggac gcagaagagc tggcccatgt atgcgaccgt tccggtctca
      721 acaaagtgaa actggcggag cgcattgctt cgatcaaggc gggcctcgca gagaaggaaa
      781 tcgaaaacct gacccgaacc gataagatca cctcactcta cgtaggcatt ctcgatctga
      841 ggagccgccg ggcccgggtg atcgaagagg atactcagca tgtgcgcagg catgaagtag
      901 agcgaatcga tcatgccata gcaaagaaga cccagcagag agagaagcta ctcgcgcgca
      961 aagagaaggg ccactttgta gtgcgtcttc cctacaggga ggccgcagag ttgttggcca
     1021 tgccggaagg gagcgtgagc gtggccgtta tgcgggcatt cgaaaaacta cgcgaaatgc
     1081 aaaaaaaact gacggaagga aaataggaat atggaattca ccgtcaaaga ttatgtagct
     1141 tggcaggggc aaccgtaatg ttcgatgaag cgggcttaac gccccaggaa atcctagaaa
     1201 ttgaagaagc gttcgttcgc cagtccctgg ttagacgtgg agacgaggag tttcaggcgc
     1261 tcctttctga gcatctcatg cagggagaat tgctgcccgt gccgcaaact ctactcgata
     1321 tttcaggccg tgttctcaca aacagcgccc ccgagtttca aatacgcctt catcctgaga
     1381 atgtaatcac ttctgtagta tccgcagtcc gcgggttttt ctccgagcct ctggctctcg
     1441 atccagtcag atccgttggc ctggaaaggc gtccggctct ggacattggc aaagcaggga
     1501 gtgtcattcg ttatagaatc attcgcgaat cggaagcaga ggtgctgctt gctgttcgag
     1561 tggatgctcc gggcgcattg agagcgaatc tgagaagaga tggaagaatt gttgcttcgc
     1621 agaccatggc cattggcgaa tcttctctgg ccttcgagca tctgggagcg ggaaactaca
     1681 cgcttgaaat cacaggcagc cgagccgaat ccgtttcatt tcacatcaac agcgaagtct
     1741 gatcaaacag gtcgaacctg gtttccaatg gaaacataaa cagagcgaac gcaggccagc
     1801 tgcttgagtt gatcgatcat ggcgcgattc atggcaacac tgtagctgtt gtgcgcgcga
     1861 attactgtct gtgctccagg cgtctgttgc gatggcaaat gaaaatagac gtttagattt
     1921 cccttgtgac acttcaggat ggtctggatt tgtttgagaa cctgttcccc atctacggat
     1981 gtgagagtct gaatctgcag gtgcagtgat ttctcgagtt tttcttctac tgcttcatct
     2041 gtgagttcct tcacggattc tacgcgaagg cgaggcattg gttttgcgtc gtctttttct
     2101 acgcgcccct tgaatatcag tggagtgtct ttctgtaaga aattgcgtga ttcttcgatt
     2161 gtggatgaga acaagactgc ttcgatggat cctgttttgt cctcaattcg aatgttccac
     2221 atttctttgc cggtctttgt gattcttttg gaaatatctc ccactactcc ggcgagttct
     2281 actctgtcgt ttgctttcat gcgctgtaga ttttcgatcg ttgaaattcc ggcgcgcgaa
     2341 agtacgcggt caaatctaga gagagggtgg ccgctgagat agcagccaag catttcgcgt
     2401 tcctgacgca gaagaatgga ttcttcgaat tcgctgactc cttccccttt aggaatgaaa
     2461 tcttctgtgg ccattgcttc tccgaagaga gacacctgac cggaagctct gtcttcctgg
     2521 ctcttttgcg cgcgctggta ggcaagatcg agagaaccca tgagagcttt gcgagtgtat
     2581 ccaaagcaat caaatcctcc ggaaagtaca agcgcttcca ctgtgcggcg attgcacagt
     2641 ctcaagtcca ctctctcgag gaaatcaatg agacctttgt atttgcccga ttttctttgc
     2701 t
//
LOCUS       DAOQJL010000536         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26591_length_2698_cov_4.374953, whole genome shotgun sequence.
ACCESSION   DAOQJL010000536 DAOQJL010000000
VERSION     DAOQJL010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26591_length_2698_cov_4.374953"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLG78_17565"
     CDS             <1..1078
                     /locus_tag="PLG78_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24985.1"
                     /translation="MASSLRLIGIIKHSFWIQHGVQAALLAEMILLSLALSDRINLMK
                     VTLEKNLGELAIAHDLVATSEKKYRVLVEDTSDLIFAMDQEGMILSINHAAQRILGYE
                     PRAMAGRSIFSFIFEGGAESQSYDVMIFRDQFEKLGESVLQTRLRFASRHGEPVDLVV
                     RLERIGAIGADSETVVLGKASRHLEDVLLPAILSERGRYSIEHFFSFSELVHQRLTRS
                     LGRYFGAETSDDIGLVLREMLINAIEHGNLEITYDEKTKAQAQGKYMDLIRERQQLLP
                     YRERHVLVEYSLNHRRVWFRITDEGPGFDHRAMQAKEEERLRDLSQSHGRGIGLARAL
                     FDIVRYNESGNSLVLAKFTKETSK"
     gene            1141..1674
                     /locus_tag="PLG78_17570"
     CDS             1141..1674
                     /locus_tag="PLG78_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNE24986.1"
                     /translation="MLKRLLSVGCFPFFLLLGCASAQTMPVVEHVDLPRFMGPWYVIA
                     NIPTFIEKGAHNAVETYSLESDGTIQTVFTFRDGSFEGPEKKYNPTGTVVNKQTNSEW
                     RMQFLWPFKSEFLISYLTPDYSQTVIARTARDYVWIMARTPSIPEKDYQAILEFLSKN
                     GYDIRLIQRVPQQAVAK"
     gene            1687..2127
                     /locus_tag="PLG78_17575"
     CDS             1687..2127
                     /locus_tag="PLG78_17575"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNE24987.1"
                     /translation="MIPALVSSLHILAFGLGLGSVFMRGIYLRRIAGEGIHRMFMADN
                     VWGVAAIIWIGTGLLRAFGGLEKGTEFYLQSTLFWVKMGLLALTFTLELFPMITFIRW
                     RIQLGRGVKPDTSRALVLARVNDVEIVLVLAILFTASMMARAVI"
     gene            complement(2132..>2698)
                     /locus_tag="PLG78_17580"
     CDS             complement(2132..>2698)
                     /locus_tag="PLG78_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24988.1"
                     /translation="NALTKLPGVSSLFKFGQNLGNQTLPGLDENMGRVIREFIAKNID
                     NLTRYAESMLTGQLDEKSIRELAEHIWADYKEQKVSKFAADAGQIKSEDVSAAASLFW
                     EHFRKTELGRDLAGAAVDAFFDHAGKKTIETALSDAGFHRERIVQTLVKGAAPGIVRA
                     VESGAMGKYLDAKLGEFYNSKEAGEILG"
BASE COUNT          614 a          659 c          680 g          745 t
ORIGIN      
        1 catggcatct tcccttcggc tgattggaat catcaaacac tctttctgga tccagcatgg
       61 cgttcaggcg gctttgcttg cagagatgat tcttctctct cttgcacttt ctgatcgaat
      121 caatttgatg aaggttactc tggaaaagaa tctgggggag cttgcaatcg cacacgatct
      181 ggtggctact tcagaaaaga aatatcgcgt actcgtggaa gatacatccg atttgatttt
      241 cgcgatggac caggaaggaa tgattctttc catcaatcat gcggcccaga gaattctggg
      301 ttacgaaccg cgcgccatgg cgggacgttc tattttttca ttcatatttg aaggtggtgc
      361 cgaaagtcag tcctatgacg tgatgatctt tcgcgatcag tttgagaagc tgggcgaaag
      421 tgttctgcaa acaaggctca gattcgcctc gaggcacggt gagcctgtgg atctcgtggt
      481 gcgtctcgag agaattggtg ccattggagc ggactctgaa acagtcgttc tggggaaagc
      541 ttctcgccat ctggaagatg tgttgttgcc cgcgatcctc agcgaacgtg gacgctattc
      601 tattgaacac ttttttagtt tttctgagct ggtacaccag agacttaccc gcagtcttgg
      661 tcgttacttt ggcgcagaaa ccagtgacga cattggactg gtactgcgag agatgctcat
      721 caacgcaatt gaacacggca atcttgaaat tacctacgat gaaaagacaa aagcccaggc
      781 tcagggcaaa tacatggatc tgattcgtga acgtcaacag ctcttgccct atcgcgaaag
      841 acatgtactt gtggagtatt ctctcaacca caggagagtc tggtttcgca ttacggatga
      901 aggtccgggc tttgatcatc gcgccatgca ggcaaaggag gaggagcgtc ttcgagatct
      961 ttctcagtca cacggaagag gaattggtct ggcgcgagcg ctctttgata tagtgcgcta
     1021 caatgaatcc ggcaactccc ttgttctggc aaagttcaca aaagaaacat cgaagtaaat
     1081 cccgggtctg tttctaaaaa agaaaagccg cgcgaggctc acgtaagttt tttggatcgc
     1141 atgttaaaac gccttctctc tgtcggatgt tttccattct tcttgctgct gggttgcgcg
     1201 agtgctcaaa ccatgcctgt agtagagcac gtggaccttc ctcgttttat ggggccctgg
     1261 tatgtaatcg ccaatattcc taccttcatt gagaagggtg cgcacaatgc ggtggaaact
     1321 tactcacttg aatcggatgg caccattcaa actgtcttta cctttcgcga cggatcgttt
     1381 gaaggaccgg aaaagaagta caaccctacg ggtacggttg tgaataaaca aacgaattcg
     1441 gaatggcgta tgcagttcct ctggcccttc aaaagcgaat tcctcatcag ctatttgacc
     1501 ccagactaca gccagacagt gatcgcgcga accgcgcggg actacgtctg gatcatggca
     1561 cgaactcctt ctattccgga aaaagactat caagcgattc ttgaatttct gtcgaaaaat
     1621 ggatatgaca ttcgcctcat acagcgcgtg ccccagcaag ctgtggcgaa atgatcggcc
     1681 aggatcatga tacccgcgtt agtttcttct cttcatattc ttgcttttgg tctgggcctg
     1741 ggcagtgtgt tcatgcgcgg catctacttg cgccgtatcg caggcgaagg cattcatcgc
     1801 atgttcatgg cggataacgt ctggggcgtg gctgccatca tctggatcgg aactggacta
     1861 ctgagggcct ttggcgggct ggaaaaaggt acggagttct atttgcagag cactcttttc
     1921 tgggtgaaga tggggctcct cgcactcacc tttactctcg aactttttcc aatgatcaca
     1981 ttcattcgct ggcgaatcca gcttggtcgc ggtgtaaagc ccgatacatc tcgtgctctt
     2041 gtacttgcgc gagtgaatga cgtggagatt gtactcgtgc ttgcgattct gtttactgcc
     2101 agcatgatgg ctcgcgccgt catctaactg cttaacccag gatctctccc gcttcttttg
     2161 aattgtaaaa ttctccaagt ttcgcgtcta gatacttgcc catagcacca ctttcgactg
     2221 cgcgcacaat tcccggggca gcgcctttga ccagagtctg aacgattctt tcgcggtgga
     2281 agcctgcgtc ggagagggcc gtttcgattg tttttttgcc tgcatgatca aagaaagcgt
     2341 ctacagctgc tccggccaga tctcttccga gctccgtttt tctaaagtgc tcccagaaaa
     2401 gagaagcggc cgcactcacg tcttcgctct tgatttgtcc cgcgtccgct gcaaacttgc
     2461 tcactttttg ttctttgtaa tccgcccaga tatgttctgc cagctctcga attgattttt
     2521 cgtccagctg gcccgtgagc atggattccg cataacgggt tagattgtct atgttctttg
     2581 cgatgaattc acgtataact ctgcccatgt tttcgtccag tcctggcaga gtctggttcc
     2641 ccaggttttg tccgaacttg aagagagagg agacgccagg aagttttgta agagcatt
//
LOCUS       DAOQJL010000537         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26649_length_2695_cov_3.756439, whole genome shotgun sequence.
ACCESSION   DAOQJL010000537 DAOQJL010000000
VERSION     DAOQJL010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26649_length_2695_cov_3.756439"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1241)
                     /locus_tag="PLG78_17585"
     CDS             complement(63..1241)
                     /locus_tag="PLG78_17585"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE24989.1"
                     /translation="MQDLFVSLGAGVHQIPLIEAARAMGYMVVAVDKNLDAPGFEYAH
                     ERIQRSILRPASIRRKLEAIAGDPVAIVARSYGKALISSAALSDHLGLQGPGVQAVRF
                     FQNKSAYKSILEEAGILVPRRFQASQIARIKKDMRLIARPVAGHGKLGIEILETRKDR
                     IRFAGRQEEGWLIEELVSGTEVTVLGASHRGSYRTLLLTSKRISQSPPHFAEIMHRYP
                     ALISSQTKEKIDQICAKIVEVSGLRTSPLVSEFIVTEDESIYLVESAPETGGEFLADF
                     AFSTATGTDYFKWFIRLLTEDSMPELNLADRAAVIRYLMPAEGQLIGLEFPKWLKSCK
                     EFAFEKKLIAPGTRTDLKHGNLHRLAAIGLRGGTSEIETLITLADKAAQETNIEYKSA
                     "
     gene            1321..2181
                     /locus_tag="PLG78_17590"
     CDS             1321..2181
                     /locus_tag="PLG78_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNE24990.1"
                     /translation="MLRGLYTGSSGMIMNQYKLDVISNNLANVDKTGFKADEAIFKSF
                     PEMVLHRTREDGLGYTPAGSFDLAPTVGKLGLGSEFNESFTRFEQGAAKQTGNPMDFM
                     IDDRGNERPSFFVVRTDKGDRLTRGGSFILDREGFLVNPDGHPLMGENGPIKVAKFNF
                     LVKENGEVWINQAVGNEPGAGANETSNQWQNPVLLDKIKVRTVEFPRELKKEGNGFYE
                     DTPESGPMVVPQERNDPTLLQGFVEASNVNLVSQMVQMIEVQRQYEANQKSVTTHDQL
                     LGRLINEVAR"
     gene            2242..2667
                     /locus_tag="PLG78_17595"
     CDS             2242..2667
                     /locus_tag="PLG78_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24991.1"
                     /translation="MKKSFLVCAVWVLALSLEGGCIQSPSPSANPDAVILLLFGSVAT
                     VRPVQATCNRTNFQCFEYNTGFTGSQMTADCASMFLGVLSASGCPTSSRLGSCYITNR
                     GYATNAFGTTAALRFETPAWAFGTAQGTCSAYGGTFAQN"
BASE COUNT          616 a          649 c          728 g          702 t
ORIGIN      
        1 cgtagtgtct atcccaggcg ctgcgattgg gaagcacatc cggaacggaa atgggtggca
       61 cgttaagcgc ttttgtattc aatgtttgtt tcctgtgcgg ctttgtccgc gagtgtgatg
      121 agggtttcga tttcagatgt tcctcccctg agcccgatgg cggccagtct atgaagattg
      181 ccatgtttga gatctgttct cgtgccgggt gctatgagct ttttttcaaa ggcaaactct
      241 ttgcatgact tgagccattt ggggaattca agtccaatga gttgtccttc tgccggcatc
      301 aggtagcgaa tgaccgccgc ccgatctgct agatttagtt caggcatgga gtcttctgtc
      361 aaaagtcgaa tgaaccattt gaaatagtcc gtgcctgtgg ctgtgctgaa tgcaaaatcg
      421 gccagaaatt cgccgcctgt ttcgggtgcc gattctacca gatagatgct ttcatcttcc
      481 gttacaatga attcgcttac gagaggagag gtgcgaaggc cggaaacttc cacaatcttc
      541 gcacaaatct gatcgatctt ctcttttgtc tgagatgaga tcaatgccgg ataacggtgc
      601 atgatttctg caaaatgggg aggggattga gatatccgtt ttgaagtgag aagtagagta
      661 cggtagctgc cgcgatgact tgcaccgagt acagtaactt ccgttccgct tacgagctct
      721 tcaatcaacc agccttcttc ctgcctgcct gcgaatctga ttctatcttt gcgtgtttcc
      781 aggatttcga ttccgagttt gccgtgtcct gcgaccggcc gcgcaatgag tcgcatgtct
      841 ttcttgatgc gcgcgatctg cgaagcctgg aatcttctcg gaaccagaat gcctgcctct
      901 tctagaatgg atttatacgc gctcttgttc tgaaagaatc ggactgcttg aactccgggc
      961 ccctggaggc ccaggtgatc ggacagagcc gcgctggaaa tgagcgcctt tccataggat
     1021 cgagcaacga tggccacagg atctccggca atcgcctcga gctttctgcg aatcgaggcc
     1081 ggtcgaagga tgcttctctg gatcctctca tgagcatact cgaacccggg cgcatccagg
     1141 tttttgtcta ccgccacgac catgtagccc atggctctgg cggcctcaat cagcgggatc
     1201 tggtgaacgc ccgcgcccag agatacaaat aagtcctgca tctaattaag cttcggcgcg
     1261 gagacataaa aatgtaggca aaatgattca gatcgggcgt agaatgccga tttcttagct
     1321 atgcttcgcg gactttacac cggttcatcc ggtatgatca tgaaccagta caagctggat
     1381 gtcatttcaa acaatctggc aaacgtggat aagacaggct tcaaggcgga tgaagcgatc
     1441 ttcaaatcgt ttcccgagat ggtgctccat cgcacacgcg aagacggtct cggatatact
     1501 ccggcgggca gtttcgatct ggctccaacg gttggcaaac tgggtcttgg ttctgaattc
     1561 aacgaaagct tcacgcgatt cgagcagggt gcagccaaac agacaggcaa ccctatggat
     1621 ttcatgattg atgatcgtgg caatgagaga ccttccttct ttgtggttcg cacggataaa
     1681 ggcgatcggc tgactcgcgg tggttctttt attctggatc gagaaggctt cctggtaaat
     1741 ccggatgggc atcctttgat gggtgagaac gggccgatta aagttgcaaa atttaacttt
     1801 ttagtaaaag aaaacggaga ggtctggatc aatcaagctg ttggaaacga gcctggcgct
     1861 ggagccaatg aaacttcgaa tcaatggcag aaccctgttc tcctggacaa aatcaaagtg
     1921 cgcactgttg agtttcctcg cgagctgaag aaagaaggga acggtttcta tgaggacacg
     1981 cctgaatccg gcccgatggt tgtccctcaa gaacgcaatg atccaactct attgcagggc
     2041 ttcgtagaag cgtctaacgt gaacctggtt tcacaaatgg tgcagatgat tgaagtacag
     2101 cgtcagtatg aggcaaacca gaaatcagtg accacgcatg atcaattgct gggtcgcttg
     2161 atcaatgaag tggctcgtta gcgatttgct tcggttttta tcttgacgac tccgcggtcc
     2221 tgaccgccat tcttcatcgg aatgaagaag agttttcttg tttgcgctgt ctgggtcctg
     2281 gccttgagtc tcgaaggcgg atgcattcaa tcgccttccc cgagcgcgaa cccggatgct
     2341 gtcatcttgc ttctgttcgg ttcggtggcc acagtgcgtc cagtgcaggc cacgtgcaac
     2401 agaactaact ttcagtgctt tgaatacaat actggtttta ccgggagcca aatgactgca
     2461 gactgcgcga gtatgtttct tggagtactg agcgcgtcgg gatgcccgac ttccagtcgc
     2521 ctggggagtt gctacattac aaaccgcgga tatgctacca atgcgtttgg aacaacagca
     2581 gcgttgcgct ttgagactcc ggcgtgggca ttcgggactg cgcaggggac ctgtagtgcg
     2641 tatggcggca cgttcgcaca gaattgatct ttctgattta tttgccgagg agcat
//
LOCUS       DAOQJL010000538         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_26863_length_2682_cov_3.508946, whole genome shotgun sequence.
ACCESSION   DAOQJL010000538 DAOQJL010000000
VERSION     DAOQJL010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26863_length_2682_cov_3.508946"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLG78_17600"
     CDS             complement(<1..263)
                     /locus_tag="PLG78_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24992.1"
                     /translation="MSVEIFHRVTLDLASALRDDPEGMATAYAGEPLPEQVPLIYRPP
                     GKKESPQVQSDLSSPGSPGKQPGKPPVREIAKKHLPANFTCEL"
     gene            complement(263..1612)
                     /locus_tag="PLG78_17605"
     CDS             complement(263..1612)
                     /locus_tag="PLG78_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE24993.1"
                     /translation="MKIYVVDDDNSIRASLKEVLQDEDFDVEDFATGKNMLKALSRER
                     PALILLDVWMGKEDGLDILGKVKEEYPALPVVMISGHGTIEQAVQATKKGAVDFIEKP
                     LSLDHVLQRINEILKIEPAVRDQRKRPDIKLEFDEIIGESTQVQAVKRAIAQAAGTNA
                     RVFIYGENGSGKELVARAIYNNSRRVGFPFVEVNCAAIPEELIESELFGHEKGSFTGA
                     QEKRIGKFEMAHQGTLFLDEICDMSMATQARVLRALQEQRFTRVGGSENIEVDVRIIA
                     ATNIDPEKAIAQGRFREDLYYRLNVIPIHLPALRERTSDVPLLLDHFLRSAARENGIP
                     IKRFSQGAMEMLQMYAWPGNVRELKNVVERLSIMSQDEEITVDTVTEHLRPPNRGQEQ
                     TPIGDLKKARDDFERTYIIQALKQHDKNITRAAKFLGMDRTNLHRKLKSLEIDPDTL"
     gene            complement(1609..>2682)
                     /locus_tag="PLG78_17610"
     CDS             complement(1609..>2682)
                     /locus_tag="PLG78_17610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE24994.1"
                     /translation="RETHIAESHPEYRDSIKKIDRVGVSLDNREQWKERIPARIRMGL
                     GLIFLFMICASLVGAVWLASSISTPIVTIAAATREVTDGRLDTRIEMEAEGELGILID
                     SFNQMTVELQSLRTRLLHSQRVAAWQEVAKRLAHEIKNPLTPIQLSADRMVRRLERPE
                     KGDLQQIVSSGARTIREQVQILKTMVEEFADFARMPEPRPVLQSLDTIAGEIGALYQS
                     VPGVKMEIRLAGNLPDIELDKNMVVGMINNLVKNAIEAIAAKPDEPGRIVLSTALHTQ
                     AGRKFVMLRVEDSGPGIDEGMRDRVFEPYYSTKGEHGSGLGLALVERTVLDHNARITV
                     SRSALGGAEFRILFPIPMEGRKS"
BASE COUNT          591 a          712 c          646 g          733 t
ORIGIN      
        1 catagctcac atgtgaaatt tgcaggaagg tgtttctttg cgatttctcg tacaggtggt
       61 tttccgggtt gtttccccgg agatccgggt gaacttaaat cgctttgaac ctggggcgat
      121 tctttcttgc ccgggggcct gtagatgaga ggcacctgct ccggcaaggg ctcacctgca
      181 tacgcagtag ccatgccttc cggatcgtct ctgagagcag aggccaagtc gagcgtgact
      241 ctgtggaaaa tttctacgct catcacagag tatccgggtc aatttcaagt gatttcaatt
      301 tgcggtgtag attggttcga tccattccca gaaacttggc cgctcgagtg atgttcttat
      361 cgtgttgttt gagtgcctga atgatgtaag ttcgttcgaa atcgtctcgt gcttttttca
      421 aatcgccaat aggagtctgt tcctgccctc gattcggtgg tcgcagatgc tctgtaacag
      481 tatccaccgt aatctcttcg tcctgactca tgatcgagag tctttccacg acgttcttga
      541 gctcgcgcac atttcctggc caggcgtaca tctggagcat ttccatggca ccctgcgaga
      601 atctcttgat cggaattcca ttttccctgg cagcagagcg aaggaaatga tcgagcaaca
      661 atggaacatc tgaagttctt tctctaagcg cgggaagatg aatcgggatt acgttgagtc
      721 tatagtacaa atcttctcga aatctaccct gcgcgattgc cttctccggg tcaatgtttg
      781 tagcagcgat gattcgaaca tccacctcta tgttctctga tccgcccacc ctggtgaatc
      841 gttgctcttg aagtgcgcga agtacgcgag cctgagtggc catggacata tcgcagatct
      901 catccaggaa aagagtaccc tgatgagcca tctcgaattt gccaatccgc ttttcctgtg
      961 cgcccgtaaa cgatcctttc tcgtgaccaa agagttcgct ctcgatcagt tcttccggga
     1021 tagcggcgca gttcacttcc acaaaaggaa acccgactct tcttgagttg ttataaatcg
     1081 cgcgtgcaac gagctccttg cctgaaccgt tttcgccata aatgaacacc cgtgcgttgg
     1141 ttccggctgc ctgcgcgatc gcgcgcttga ctgcctgcac ctgagtggat tctcctatga
     1201 tttcgtcgaa ttcaagtttg atgtcgggac gctttctctg atctcgaaca gccggttcga
     1261 ttttgagtat ttcattgatt ctctggagca catgatcgag agaaagtggt ttttcaataa
     1321 aatcgaccgc accttttttc gttgcctgca ccgcctgctc gatggtaccg tgaccagaaa
     1381 tcatcacaac aggaagcgca ggatattcct cttttacttt tccaagaata tccaggccgt
     1441 cttcctttcc catccacaca tcaagcaaga taagagcggg gcgttcgcgc gagagtgctt
     1501 tgagcatgtt tttgcccgtt gcaaaatcct caacgtcgaa gtcctcgtcc tggagaactt
     1561 ccttgaggga agcgcgaatg ctgttgtcgt cgtcaacgac gtagatcttc atgacttgcg
     1621 accttccatc gggatgggga atagaatgcg aaactctgct ccacccagag cgcttcttga
     1681 aaccgtaata cgcgcattgt gatcgagaac cgttctttcc acaagtgcaa gccccagccc
     1741 acttccatgc tcccccttcg tagagtaata aggttcaaaa acgcggtctc tcatcccttc
     1801 gtcaatcccg ggtccggaat cttccactcg aagcatcaca aattttctgc cggcctgagt
     1861 gtgcagtgcc gtggaaagta caatgcggcc gggttcatct ggtttagccg cgatggcctc
     1921 aattgcattt tttaccaggt tgttgatcat accaacgacc atgttcttat cgagctcaat
     1981 atccggcaga tttccggcaa gacgaatctc catcttcacc ccgggaacac tctgatagag
     2041 agcaccaatt tctcccgcga tcgtgtctag agactgcaga accggcctgg gttccggcat
     2101 gcgggcgaaa tccgcaaatt cttccaccat tgtttttagt atctgcacct gctcgcgaat
     2161 agttcgcgct ccgctcgaaa cgatttgctg cagatctcct ttctcaggtc tttcgagtct
     2221 acgaaccatt ctatctgcgg agagttgaat cggagtgaga ggatttttga tttcgtgtgc
     2281 aagcctcttg gcgacttcct gccaggcggc caccctctgg ctgtggagca atcgcgtacg
     2341 aaggctctgc aattctacag tcatttgatt gaagctatca atgagaatcc ccagttctcc
     2401 ttctgcttcc atttctatgc gagtatcgag tctgccgtct gtgacttcgc gcgttgcggc
     2461 agcaatggta acgatgggtg tggagatgga acttgccagc cacacagcac ccaccagcga
     2521 agcgcagatc atgaacaaaa agatcaaacc cagacccata cggatgcgag cgggaattcg
     2581 ttccttccat tgttcgcggt tgtcaaggga aacacccacg cgatcaatct tcttgatgga
     2641 gtctctatat tccggatgag attcggcgat gtgagtttct cg
//
LOCUS       DAOQJL010000539         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27354_length_2654_cov_3.264332, whole genome shotgun sequence.
ACCESSION   DAOQJL010000539 DAOQJL010000000
VERSION     DAOQJL010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27354_length_2654_cov_3.264332"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..210
                     /locus_tag="PLG78_17615"
     CDS             19..210
                     /locus_tag="PLG78_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNE24995.1"
                     /translation="MSDTNARLSAQDVESPCNDICIMDEYTGYCRGCLRTIDEIARWA
                     SYSPEQKREVLLKIKERAE"
     gene            207..1433
                     /locus_tag="PLG78_17620"
     CDS             207..1433
                     /locus_tag="PLG78_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE24996.1"
                     /translation="MRQYWKRFLVFFSFVTTVLGIAYSFVAVRLTVGLVHPGLIALVW
                     ILAAVSLLLAPLAFALRMFQVPERIEKIIAWTSYLSFGFFAMLFALIASRDLGLVAVD
                     QVGGIVDRFLPWRDPANLLHPMHREFLVATSNAALLGLAGGMFVYGVIRARAIGIERV
                     QVPIDGLPESLHGFTIAQITDIHIGPTLKRSFALEIVKKVNNLKADMIAVTGDVVDGS
                     VQQLRDHTEPFADLEARHGVFFVTGNHEYYSGALSWISEFRNLGFQVLLNEHSVLEHA
                     PGGNVMVAGVTDYRAAEFFDDHRSDPALALRRAARGSRSSKEPHVKILLAHQPRSIAG
                     AADAGFDLQISGHTHGGQFFPGPYLVRLQQPYTKGLHRHGDKTWIYISRGTGYWGPPI
                     RVGASPEITLIELVRR"
     gene            complement(1497..2384)
                     /locus_tag="PLG78_17625"
     CDS             complement(1497..2384)
                     /locus_tag="PLG78_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE24997.1"
                     /translation="MHRIGVDLGGTKAESILLSGSGQELFRKRIPTRREEGYEAILSD
                     LTALVQETAVAGKLDLNECTVGIGIPGIIDQDGRVVNANTTCLIGHAFESDLARNLNH
                     PVLMMNDANCFTMAECTSGAGQGYDFVFGVILGTGCGGGISIGGKVRRGLHGIAGEWG
                     HVSVDPDGTMCYSGIKGCIESRISGSGVEKRFADLTGQKKLLKDIVIGMREGDPVCQE
                     HMEFFFEEFGRCMAVIINILDPDAIVLGGGVSQIPELYTIGVERIKKYTFYKNPKTPV
                     LQNKLGDSAGVFGAAWIGV"
     gene            complement(2377..>2654)
                     /locus_tag="PLG78_17630"
     CDS             complement(2377..>2654)
                     /locus_tag="PLG78_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNE24998.1"
                     /translation="GLFQELFHSLTYGGDKYFLLADYPSYVDVQNRISRDFVDRPGWT
                     KKSILNAARSGKFSSDRTIGEYANEIWHAKPIRQRSPRLQAVLDEHA"
BASE COUNT          604 a          666 c          679 g          705 t
ORIGIN      
        1 tgagggaact ttctcagtat gagtgatacg aatgctcgcc tgtcggcgca ggatgtggaa
       61 tctccgtgca atgatatatg catcatggat gaatatactg gatactgcag aggctgcttg
      121 cgcaccatag acgagatcgc acgctgggct tcctattcgc ctgaacagaa aagagaagtt
      181 cttctgaaaa tcaaagagag ggcagagtga gacagtactg gaaaagattt ctcgtattct
      241 ttagttttgt aactactgtt cttggaattg cctattcttt tgtggcagtg cgcctgacag
      301 tcgggctggt tcatccgggg ctcatagcgc tggtctggat tctagcggca gtctctctgc
      361 ttctggctcc tcttgcgttt gctcttcgca tgttccaggt ccccgagaga atcgagaaga
      421 tcatcgcgtg gactagttac ctttcctttg gtttttttgc catgctcttt gctctgattg
      481 cttcgagaga tctgggtctt gtggctgtag accaggtggg tggaattgtg gatcgatttt
      541 taccgtggag agatcccgca aatctgcttc atccaatgca tcgtgaattt ctcgtggcca
      601 caagcaatgc ggcactgttg ggtctggccg gtggcatgtt cgtgtatggt gtgattcggg
      661 cgcgggccat tggaattgaa agagtccagg tacccatcga cggcctgcca gagtctttgc
      721 acggttttac cattgcgcag atcacggaca ttcacatagg cccaacactg aagagaagct
      781 tcgcactcga aatagtaaag aaagtaaata atctgaaagc ggatatgatc gcagttaccg
      841 gcgatgttgt agacggaagc gttcaacagt tgcgcgatca caccgaacct tttgcggatc
      901 tcgaagcaag gcacggagtg ttttttgtaa ctggcaatca cgagtattat tctggagcgc
      961 tttcctggat ttcagaattc agaaacctgg gtttccaggt tttgctcaat gaacactctg
     1021 tgttggagca tgcgccgggc ggaaatgtaa tggtggccgg agtcacggat tacagggccg
     1081 ccgagttttt cgatgatcac agatcagatc ctgctcttgc tcttcgccgc gctgcccgtg
     1141 gaagccgatc ttccaaagaa cctcatgtga aaattctact cgcacatcag cctcgtagca
     1201 ttgcgggggc ggcagacgca ggattcgatc ttcaaatttc cggccacact cacggcggtc
     1261 agttttttcc agggccctat ctggtgcgat tgcagcaacc atacacgaaa ggattgcatc
     1321 gtcatggaga taagacctgg atttacatca gccgcggaac tggatactgg ggcccgccga
     1381 ttcgagtggg tgcttctcca gagatcactc tgattgagct tgttcgtcga tagtgctcag
     1441 tcggtgcgtc gttcatgcgt cgctagcgcg cggatggcct gcaggccgca gagcaatcaa
     1501 actccaatcc aggcggctcc aaacactccg gctgaatcac caagcttgtt ttgcaataca
     1561 ggcgtctttg gattcttata aaaagtatac tttttgattc tttccacacc gatggtgtag
     1621 agttctggaa tctgagatac accgccgccc agaacgattg catctggatc cagtatgttg
     1681 atgatgacag ccatgcatct tccaaactct tcgaagaaga attccatatg ttcctgacag
     1741 acaggatcac cttctcgcat accaatcact atgtctttga gtagtttctt ctgcccggtg
     1801 aggtccgcaa atctcttctc taccccggag ccgctgatgc gcgattcaat gcatcctttg
     1861 attccactgt agcacatggt tccgtccgga tctacggaaa catgtcccca ttctccggca
     1921 attccgtgaa gcccgcgacg aactttgcca ccaatggaaa ttcctccacc acatccagtg
     1981 ccaagaatca caccgaatac aaaatcgtat ccctgtcccg cgcccgatgt gcattctgcc
     2041 atggtaaaac agtttgcgtc attcatcatg agcactggat gatttagatt tcgcgcgagg
     2101 tctgattcaa atgcatgacc aatgaggcat gtggtattcg cgtttacgac gcggccatcc
     2161 tgatcaatga tcccggggat tccaattcca accgtgcact cgttcagatc cagttttcca
     2221 gcaacagcag tttcctgcac gagtgctgtg agatcggaga ggatggcttc gtatccctct
     2281 tctcgtctgg ttggaattct ctttcgaaaa agttcctggc cggagccaga gagcaggatg
     2341 ctctccgcct tggttccgcc caggtccact ccgattctat gcatgctcgt ctaaaacagc
     2401 ctgcaggcga ggcgatctct ggcggattgg ttttgcatgc cagatttcgt ttgcgtattc
     2461 tccaatcgtg cgatcggaag aaaactttcc agagcgcgct gcgttgagga tggacttctt
     2521 ggtccagccc ggtctatcga cgaaatcgcg cgaaatgcga ttctgcacat ctacatagga
     2581 tggatagtcc gcaagcagga agtatttgtc tccgccgtag gtcagagaat ggaacaattc
     2641 ctgaaagagg ccat
//
LOCUS       DAOQJL010000540         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27394_length_2652_cov_4.306122, whole genome shotgun sequence.
ACCESSION   DAOQJL010000540 DAOQJL010000000
VERSION     DAOQJL010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27394_length_2652_cov_4.306122"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLG78_17635"
     CDS             complement(<1..362)
                     /locus_tag="PLG78_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE24999.1"
                     /translation="MKIRVTLLRNRRIPFLCVEVLNREAFGRITSVLRQIPGSSYLAK
                     RKSWILPLNQRTMAELLPLGYRGRYEKLELLRREMSIRKYSASTMRSYLALNRNFLIY
                     AGVPHPEIRDRHVQMFLD"
     gene            682..1554
                     /locus_tag="PLG78_17640"
     CDS             682..1554
                     /locus_tag="PLG78_17640"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNE25000.1"
                     /translation="MTEPESILARKVIVTCALTGVAATRKQCPAIPYTPAEIGEEARR
                     AYEAGASVVHIHAREDDGSPSHRVEVYGEIMKEVRKRSPVLINFSTGAVEVPKEERVR
                     HITELRPEIGALNMGSMNYAKYNSTKKGFVFNWVFENSFDTIQFFLDAMVKSGVKPEL
                     ECFDVGHCYNIRPFLDMGLVKGPLQFSFIMGVLGGVPATAKNLAAMAEQIPEGSVWEV
                     IGISHVQWLMIASALALGGNIRVGLEDNFYVAPNVMAKSNGDLVEKAVRMARDTGREP
                     ATVEEAKSLLSLAG"
     gene            complement(1570..2364)
                     /locus_tag="PLG78_17645"
     CDS             complement(1570..2364)
                     /locus_tag="PLG78_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25001.1"
                     /translation="MQKLKTVTSLESILEAWDLYANRKFEAIVSHEEYSHSPSKDLRD
                     LARLGQLEIARNTTDPEGELFAPLVQAMRQYWKGHYSDACALLGQWLETREYHTAMIL
                     DRFLECAFMCDQYRPAFQILNKFIKIPYYRDRLADDIVRSAHHAGLDKEAVGLYRDLA
                     GSIRDQSVHQKAALSMVKLGLFRDAEKLLIKLFEKITGKKYGYSEDQFDALRSKYSER
                     ASTIEKQKRASSEDVMELGLAYLFSSRYDEALRLFETLKKKSASAA"
     gene            complement(2421..>2652)
                     /locus_tag="PLG78_17650"
     CDS             complement(2421..>2652)
                     /locus_tag="PLG78_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE25002.1"
                     /translation="GFRQFTRQEARSLDIEGFVTNRPDGSVECLARGSRESMDQFQSK
                     LKKGPPLSRVESIVATPETTFPAGIKAGFQIL"
BASE COUNT          632 a          608 c          746 g          666 t
ORIGIN      
        1 tagtccagaa acatttgaac atgtcgatct ctgatttcag gatgagggac gcccgcatag
       61 atcaggaaat ttctgttgag cgccagatac gatcgcatgg tggacgcgct gtatttgcga
      121 atggacattt cgcggcgaag caattccaac ttttcatagc gacctctata gcccagagga
      181 agaagctctg ccatggttct ctggttgaga ggcagaatcc aggacttgcg ctttgctaaa
      241 taagaagaac ccgggatttg tcgtaaaaca ctggttatgc gaccgaacgc ctctctattc
      301 aagacttcaa cacacagaaa agggatgcgg cgatttcgca gtaaagtgac tcggatcttc
      361 actctactgg aatgtggtgc gaatttgaat cgttccgggc gaatcggggc cgtgttcgca
      421 tggcttcgcc aggtgactcc caggagccat ttttcccgtt tcagggtatt agattcgacg
      481 agcagagttt cgcggcgcaa gttttatttt gaaaacatct tcattattga gcatttcacg
      541 cgacgcgtcc ggaatgaatg ctacgtggga gggaatgaac gtcacagggc gagtgaccga
      601 agggtgaggt ctggcggaag gggcgaaaac cctcgatgga taagtgagtg caaggccaca
      661 gggcggcgta aaaacgtccg catgactgaa ccagaaagca ttcttgctcg caaagtaatt
      721 gtaacctgtg cattgacagg agtggccgcg acccgcaaac aatgcccggc gatcccctat
      781 acgcctgcgg aaattggaga agaggcgcgc cgcgcgtatg aagcgggggc atccgtggtc
      841 cacatacacg cccgcgaaga tgacggaagt ccatcgcaca gagtggaagt gtatggcgaa
      901 atcatgaaag aggtgagaaa gcgcagtcca gtgttgatca acttttccac aggtgcggtg
      961 gaagtgccaa aagaagagcg cgtgcgtcac attacagaat tgcggccgga aatcggagct
     1021 ctgaacatgg gttcgatgaa ctatgcaaag tacaactcta cgaagaaagg ttttgtgttc
     1081 aactgggtat ttgagaactc gtttgatacg attcaattct tcctggatgc gatggtgaaa
     1141 tcgggtgtga agccagaact agaatgcttt gacgtgggcc attgctataa cattcgtccg
     1201 ttcctggaca tgggtctggt caaaggtccg cttcagtttt ccttcattat gggcgttctc
     1261 ggtggagttc cggcaacagc aaagaacctg gcggctatgg cggagcaaat cccggaaggt
     1321 tccgtctggg aagtcattgg aatcagtcac gtgcaatggt tgatgatcgc aagcgctctg
     1381 gcacttgggg gaaacattcg cgttggactg gaagacaact tctatgttgc tccgaacgta
     1441 atggcaaaat caaacggaga tctggtagaa aaggctgtga gaatggctag agacactggc
     1501 cgggagccgg ccacagtgga agaggcgaaa tctctcctgt ccctggcagg ttagttcgtt
     1561 tacggatcgt taggcggcag aagcggattt cttctttaac gtttcaaaca ggcgaagtgc
     1621 ttcgtcgtat ctgctcgaaa acagataggc gaggccaagt tccattacat cttccgaaga
     1681 ggcgcgtttt tgtttttcta tggtagaggc tcgctctgag tacttgctgc gcagtgcatc
     1741 aaactgatct tcagaatagc catacttttt tccggtgatc ttttcaaaca gtttgatgag
     1801 cagtttttcc gcatcgcgaa agaggcccag cttgaccatg gacagagctg ccttctggtg
     1861 aacagactgg tctcgaatcg accctgctag atcacgatag agacccacag cctctttgtc
     1921 gagccccgcg tggtgagcgc tacgcacgat atcatctgcc agtctgtctc tgtagtaagg
     1981 aatttttata aatttgttca atatttgaaa agcaggtctg tactggtcgc acataaacgc
     2041 gcattcaaga aagcggtcaa ggatcatggc ggtatgatat tctctggttt cgagccactg
     2101 acccaggagg gcgcatgcgt cgctgtagtg gcccttccag tattgcctca tggcctggac
     2161 gagaggagcg aagagctcgc cctcaggatc tgtggtgttg cgagcgatct cgagctgtcc
     2221 aagtcttgcc agatcgcgca gatctttgga gggagagtgc gagtattctt catgcgaaac
     2281 aatcgcctcg aactttctat tcgcatagag gtcccaggct tctagtatgg attcgaggga
     2341 agtaaccgtt ttgagttttt gcatggccgc ctgctatttg cttcggtggg aagcggcctt
     2401 tctataaccg ttttttggcc ttacaggatc tggaatccgg ccttgattcc tgctggaaat
     2461 gtggtttctg gagtagccac gattgattct actctggata gaggggggcc ttttttaagc
     2521 ttcgattgga actggtccat gctttcccgg ctgcctcgtg ccaggcattc tacgcttcca
     2581 tcgggtctgt tggttacgaa gccttctatg tccaggcttc tggcctcctg gcgagtgaac
     2641 tggcggaacc ca
//
LOCUS       DAOQJL010000541         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27469_length_2647_cov_4.396991, whole genome shotgun sequence.
ACCESSION   DAOQJL010000541 DAOQJL010000000
VERSION     DAOQJL010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_2647_cov_4.396991"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLG78_17655"
     CDS             complement(<1..530)
                     /locus_tag="PLG78_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25003.1"
                     /translation="MKRSYIIGGLVAIAVIVTLVLLYSGGSGKRGRLDSSPFGDSPIF
                     RRGSEGFLSPRELEKMLAKGSSPKGELEMYRTYSQYPHDSRPLTASMKDLTNPWFVEQ
                     VPLPIPAPGAANEQEIKELVEKMQASGKSNDDIEKEIAKMIENQPAFQFVLNRHTVTE
                     GDTLVATLKITKNGSP"
     gene            complement(642..1448)
                     /locus_tag="PLG78_17660"
     CDS             complement(642..1448)
                     /locus_tag="PLG78_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25004.1"
                     /translation="MKQAIAIALVAFALSSVSAGEYVVFVHGRSSNHCGTGTTDVNNY
                     WGNAKNISTTLTKYFVGYDGSTDPRSSGSCRAQTSMYTVMQAVCTGANSCVIICHSAG
                     CYATEYFLDKTTTTYNVKRILASSSAAGGSDLANAAFWPSDGMVTALKTSNARGSYNH
                     NNMKGIGVYAIAGYKGTFGASAILPGEDDGAVSFHSTCGINTTGSYSNCLSGTRYSYH
                     YIWNVVANNSWGSSQNGYYRTHVGDGSDSINDATKNEWTDCKNGGYAGCK"
     gene            1623..2597
                     /locus_tag="PLG78_17665"
     CDS             1623..2597
                     /locus_tag="PLG78_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25005.1"
                     /translation="MRLNSLVLLLFLTAACKEPAPPASAAIYAIAPATHVSTWDQNLL
                     GRPLPARIEPATEPLLDYIRRWNESDGFQAVPKPARMDAKDKEALARELSNLPPPVLK
                     LLQKHVITIATVEDLGGSAMAGAAFDDRGEFPFGFLILDINMFKRNANQWISYKENTV
                     FQPEPGTSYTVTLEPEVSNTRIAGMRFVILHELGHIIAQVTRSLPAFTDRRPDRNKFP
                     FARTFDEFPLRGRIKFYADKPPFSVMEAERLYSEFSPTRFPTLYSCVDEEEDFSETFA
                     LYIHTVLMKKPYEVKLIEKGKTTVLARDRILLPQMKEKRDFIARILEK"
BASE COUNT          642 a          682 c          649 g          674 t
ORIGIN      
        1 atgggagaac cgttctttgt aattttcaac gtggccacca gagtatctcc ttcggtgacc
       61 gtatggcgat tcagcacgaa ctggaaagcg ggctgattct ctatcatctt cgcaatttcc
      121 ttttctatgt cgtcgttgct tttgccggac gcttgcattt tctctacaag ttctttgatc
      181 tcttgctcgt tggcggcacc tggcgccgga ataggaagag gcacttgttc tacaaaccag
      241 ggattggtaa gatctttcat ggatgcagtg agagggcgcg aatcgtgagg atactgagag
      301 tacgtgcgat acatttccaa ttcgccttta ggtgaagatc ccttcgcgag catcttctct
      361 aactcgcgcg gagaaagaaa tccttcgctt ccgcggcgaa agataggtga atctccgaat
      421 ggactggaat ccagacgacc tcttttgccc gaaccacccg agtatagcaa aaccagtgtg
      481 acaatcactg caatggcaac cagaccgccg atgatataac ttctcttcat gtttcctctt
      541 ttgtgcttcc agagttcccg gaagcttttc atgatctctt tgctgtggtc aaaaaaaaag
      601 ccctctttcg agggctttcg tcaagcgaaa ctgtgctgga attacttgca gccagcgtat
      661 ccgccatttt tgcaatcagt ccattcgttc ttggttgcgt cgttgatgga atcgcttccg
      721 tcaccaacgt gtgttctgta gtaaccgttc tgagatgatc cccagctgtt gtttgctact
      781 acattccaga tgtagtggta gctgtagcga gtcccgctga ggcagttgct atagcttcca
      841 gttgtgttga ttccgcaagt ggagtggaaa gaaacagcgc cgtcatcttc gccaggaaga
      901 atggcagaag cgccaaaagt ccctttgtaa cctgcgatcg cataaacacc aattcctttc
      961 atgttgttgt ggttataaga accacgagca ttcgaagttt tgagtgcagt aaccattccg
     1021 tcagatggcc agaatgctgc gttcgcgaga tccgatccgc cagctgcgct ggaagaagcg
     1081 agaatgcgct ttacgttgta agtggtagtg gtcttgtcca ggaagtactc agttgcgtag
     1141 caacctgcgc tgtggcagat gatgacgcag ctgtttgcgc ccgtacatac cgcttgcatt
     1201 acagtgtaca tgctggtctg cgcgcggcag cttccggaag aacgtggatc cgtagatccg
     1261 tcgtagccta cgaaatactt ggtcagcgta gtgctgatat tctttgcatt tccccagtag
     1321 ttgttcacgt ctgttgttcc tgtgccgcag tggttgctcg aacggccatg tacgaacacg
     1381 acgtattcac ctgcagatac gctcgaaaga gcgaacgcta caagtgctat agcaatagct
     1441 tgtttcattg atttcccccg tgatatggac ccgattcgct tccccggctc ttcgaatccg
     1501 cccaaccagt accggcgcag tgcaaaaagg tcaatccaaa atattgcgca ttgcacaaaa
     1561 aaatgaaatt tttttcttat gacccgactt gcgcaatcgg cacagacccg agaggttgct
     1621 ttatgcggct caatagtctg gttttattac tatttctgac cgctgcgtgc aaggaaccag
     1681 cccctccggc atccgcagcc atttatgcca ttgcgccggc cacgcacgtg tcgacctggg
     1741 accagaacct gctgggaaga ccacttccgg cgcgcattga accggccacc gaaccgctat
     1801 tggactacat ccgcagatgg aacgaatcgg acggcttcca ggctgtgccc aaaccggccc
     1861 gaatggatgc aaaagacaaa gaggccctgg cgcgagaact gtccaatctt ccgccccccg
     1921 tgctcaagct attgcaaaag cacgtcatta caatcgccac ggtagaagat ttgggcggat
     1981 ctgcgatggc aggagctgcg tttgatgaca gaggggaatt tccgttcggg tttctgatcc
     2041 tcgacatcaa tatgttcaaa agaaatgcga atcagtggat cagttataaa gaaaacacag
     2101 tattccagcc cgaacctggc acgtcataca ctgtgactct tgaaccggaa gtctcaaaca
     2161 cacgtatcgc aggaatgcga ttcgtcatcc ttcacgaact cgggcatatc atcgcacagg
     2221 tgacccgctc tctgcccgcg tttaccgatc gtagaccgga cagaaataaa ttccccttcg
     2281 cgcggacgtt cgacgaattc ccgcttcgcg gaagaatcaa gttttacgcg gacaaaccac
     2341 ccttctccgt gatggaagca gaaaggctct atagcgagtt ttctcctaca cgctttccaa
     2401 ctctgtattc ctgtgtagat gaagaagaag atttttcaga aacatttgct ctgtacattc
     2461 acacagtctt aatgaagaag ccgtacgaag tgaagcttat cgagaaaggc aagaccactg
     2521 tgcttgcgcg agatcggatt ttgttaccgc agatgaaaga gaagcgcgat ttcatcgcac
     2581 ggattctaga aaaataaagg gcggcctttt ggccgccccg cgaactatcg tgcagtgatc
     2641 tgcaatc
//
LOCUS       DAOQJL010000542         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27470_length_2647_cov_4.216049, whole genome shotgun sequence.
ACCESSION   DAOQJL010000542 DAOQJL010000000
VERSION     DAOQJL010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27470_length_2647_cov_4.216049"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLG78_17670"
     CDS             complement(<1..466)
                     /locus_tag="PLG78_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE25006.1"
                     /translation="MLRIRRFLLFSFVCAASAALLFDCAGKSGPALFAAKFGESYYPS
                     HLVNTTSTREYVKLDWLFYVIEVSGKIVLVDAGMKDESVADRFRIRGFRTAPQILKLQ
                     GIFPSQVTDIVLTHAHPDHVGGITFFPNAVVHVQDAEFQAIRYAPKLAPVKKI"
     gene            complement(454..1605)
                     /locus_tag="PLG78_17675"
     CDS             complement(454..1605)
                     /locus_tag="PLG78_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE25007.1"
                     /translation="MKKIVALIAFAFVCSLQAQTLHMLIVADTADASIGSSTLADLQN
                     MQNKAREIASNSGMKLNMKVLKSPEFNGTTTLAAVQAINPGPNDTVFFYYSGHGYRTR
                     ESKTKWPNMYVKKPQPGLEFSKVLEILDQKNPRLVIALGDLCNSFSDQSERSANLRAF
                     AELQPSNYKKLFVEFSGRVYASGSKPGQYSFAMEDGGAFTNQWLQALGTELRAEEPSW
                     KRLMAVATKPIRIGSPDQATQDPIFELKEGKQVVAHNTVDENAPTIQPTNTVVQQEVT
                     DAEEETEEYQEDELCPDLAELLGSLKQLDGALKPGIRVTRGDNLYNSLDTVNKALSSV
                     SEAYGDPKLQKSVTGLSTALEANNLQAYKKNLGRVIQYFEKLNQDKCSE"
     gene            complement(1607..2134)
                     /locus_tag="PLG78_17680"
     CDS             complement(1607..2134)
                     /locus_tag="PLG78_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25008.1"
                     /translation="MRFFSAAILAMAMLNCQTTAKGVPVQPSAKVKEFLGDSILGVLS
                     APDSVTAVLVEPRGGSTADHMDGFKLLSKPVALTAEQISTLQNAIMDEKTYDFLHSKR
                     HPFDPSVGFQFKKGKDTVNVLVSYYGNLWQFTGSGKTLIEDNDGARKPLLNLAKQLFP
                     KDKDIQSFKETMEGQ"
     gene            2236..>2647
                     /locus_tag="PLG78_17685"
     CDS             2236..>2647
                     /locus_tag="PLG78_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25009.1"
                     /translation="MKSHASFLRRVSVSASFALFAGCQFFTGPKITDEIVDKYVKAYT
                     GLRQIGPELAEKMKNAQGREVDIDPGMQGYAQIEKIIKDAGFKDYKEFVQVNAKIGLA
                     FSQMEGRTFMDQMDKMHSIGYEEIDKQLADPQVPE"
BASE COUNT          652 a          636 c          661 g          698 t
ORIGIN      
        1 aaatcttttt cacaggcgcg agcttgggcg cataacggat ggcctggaat tccgcatcct
       61 gaacatgaac cactgcattg ggaaagaagg taattcctcc cacatgatcc ggatgtgcat
      121 gagtcagaac aatgtcggtg acctgcgaag gaaaaatgcc ctggagtttc aagatttgag
      181 gcgcagttcg aaaaccgcga atgcgaaagc gatcggccac tgattcatct ttcatgcctg
      241 catctacgag aacgatcttg ccggatactt caatgacgta gaagagccag tccagcttca
      301 cgtattcacg tgtgctcgtt gtatttacaa gatgagacgg gtaatagctt tcgccgaatt
      361 tggcggcaaa aagcgccggc ccacttttgc cggcgcaatc aaacaaaagc gccgcagatg
      421 cggcgcatac gaacgagaat aggagaaacc tccttattct gagcacttat cttgattcag
      481 cttttcgaaa tattgaatga ctcttccgag gtttttctta taagcctgaa gattgtttgc
      541 ttccagtgcg gtggagagtc cggtcacgct cttttgaagc tttggatctc cgtatgcttc
      601 ggaaacactg ctaagtgctt tgttcaccgt atccagactg ttgtagagat tgtctcctcg
      661 ggtaacgcga atccctggct tgagagcacc gtccagttgc ttgagagaac cgagcagctc
      721 tgccagatct ggacagagtt cgtcttcctg atactcttcc gtttcttctt ccgcatccgt
      781 tacttcctgt tgcactactg tattggttgg ctgtatggta ggggcgtttt catcgacagt
      841 gttgtgcgca actacttgct tgccttcttt caattcgaag atcggatcct gcgttgcctg
      901 gtcgggagaa ccgatgcgaa ttggctttgt agccacggcc atcaaccttt tccagcttgg
      961 ttcctccgcg cgaagctcag tgccgagcgc ctgcagccac tgattggtga acgcaccgcc
     1021 gtcttccatc gcaaaggaat actgccctgg ctttgaacca gatgcataca ctctaccgct
     1081 gaattccaca aacaacttct tgtagttaga aggctgaagt tccgcgaatg cgcgaagatt
     1141 cgcactgcgt tcgctttgat cagaaaagct attgcacaaa tccccgagag cgatcacaag
     1201 gcgtgggttc ttctgatcca gaatctcaag cacttttgaa aactcaagcc cgggctgtgg
     1261 ctttttcaca tacatgtttg gccattttgt ttttgattcg cgcgttctat atccatgtcc
     1321 ggagtaatag aaaaacacag tatcattcgg acccggattg atggcctgaa ctgctgcgag
     1381 tgtggtggtt ccattgaact ctggagattt gagaactttc atgttcaatt tcattccgct
     1441 gttggaagcg atctcgcgag ctttgttctg catgttctgc agatccgcga gagtggaact
     1501 accgatgctc gcgtccgctg tgtccgcaac gatgagcata tgcagagtct gggcttgaag
     1561 cgaacagaca aacgcgaacg ctatgagtgc aacgatcttt ttcattctac tgaccctcca
     1621 tcgtttcttt gaaactctga atatctttat ctttggggaa gagttgcttt gcaagattca
     1681 ggagtggctt gcgtgctccg tcattgtctt cgatgagagt cttgccgctt ccggtgaatt
     1741 gccagaggtt tccgtagtag ctcaccagca cattgactgt gtcttttccc ttcttgaatt
     1801 ggaagccaac agaaggatca aaaggatggc gtttactatg aaggaaatcg taggtttttt
     1861 catccatgat tgcattctgc aaggtggaga tttgctccgc ggtcagtgcg acaggtttgg
     1921 aaagcagctt aaagccatcc atatggtccg cagtggatcc tcctctgggc tcaacgagga
     1981 cagctgtaac cgaatcagga gcagacagta cgccgaggat cgaatcccct agaaattcct
     2041 ttacttttgc cgatggctgc acaggcaccc ccttcgccgt ggtctggcaa tttagcattg
     2101 ccatggccag aatggccgct gaaaaaaatc gcattgataa ctcctgtatt tttgattcag
     2161 attgaatcgc gatgttcgtc tgctcaacaa gaaaaaaggt tcgacttaat tttcctttga
     2221 aagccagact gagacatgaa atcgcacgcc tccttcctcc gccgggtgtc tgtctctgcg
     2281 tctttcgcgc ttttcgctgg atgccaattc ttcactggtc caaagatcac agacgaaatt
     2341 gtagacaaat acgtaaaagc atatactggc ctcaggcaaa tcggccctga actggcagaa
     2401 aaaatgaaga atgcgcaggg gcgcgaagtg gacatcgatc cagggatgca gggatatgcg
     2461 cagatcgaga agatcatcaa ggatgcagga ttcaaggact acaaggaatt cgtgcaagtg
     2521 aatgcgaaga tcgggctggc tttcagtcaa atggaaggca gaacgttcat ggatcagatg
     2581 gacaaaatgc acagcatagg atacgaggag atagacaaac agctggcaga tcctcaagtg
     2641 cccgaag
//
LOCUS       DAOQJL010000543         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27477_length_2647_cov_3.183642, whole genome shotgun sequence.
ACCESSION   DAOQJL010000543 DAOQJL010000000
VERSION     DAOQJL010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27477_length_2647_cov_3.183642"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..711
                     /locus_tag="PLG78_17690"
     CDS             184..711
                     /locus_tag="PLG78_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNE25010.1"
                     /translation="MRLQWITLLLSVALFATACKDKSGPAGGGIANVSDEESAKTVLQ
                     IAAGSKDHTTLVKAVQAAGLENSLANQGPFTVFAPTDAAFKALPSGALDKVMADKNLL
                     TTVLGYHVYVGSSLKESMLTDGDELSMFDGGTAKISVKGGKISINGANVLAAVPASNG
                     MVYVIDKVLLPPEKK"
     gene            919..1350
                     /locus_tag="PLG78_17695"
     CDS             919..1350
                     /locus_tag="PLG78_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE25011.1"
                     /translation="MKIGTALVTALFLAVALAACKKQSGDNASNDSGTGIGPVSSVQI
                     AAGIDEGMAKKGADLFEAKCSACHKWEEKVVGPALQGVTTRRKPEWIMNMILNPVEMT
                     QKDPTAQKLLEEHLTQMTFQNVTQEEARMILEHFRKKDGAK"
     gene            1361..>2647
                     /locus_tag="PLG78_17700"
     CDS             1361..>2647
                     /locus_tag="PLG78_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HNE25012.1"
                     /translation="MKRLQIISSVFFALVAFVACGKAGTAGLASDAAERVYVKPGDKD
                     EIYMFASGGFSGQVGVNGIPSGRLIRVIPVFSQWPETGYGYDETTKNMLMTSHGFVPW
                     DDSHHPQLSVTEGKQDGRWLFINGNNTPRIARIDLKSFETVEIIELPNSAGNHPSSFL
                     TENSEYALAGTRFSIPIPNVPMAWEQFQKEMKAGMSFVSIDKTTGKMSIDFQVLLPGI
                     SYDLGRCGKGPSHDWCFISSYNTEQAYKMLEVNASKKDKDFVMAINWKKAKACVDQGK
                     ARMFPGAYRHNYREENQEAVSESLNGTKMLDPKDCPGMVFYMPTPKSPHGSDVDPTGE
                     YIVAGGKLATVIPVHSFSKMLKAIEDKAFSGEFFGIPVLKYEATLAGEVQKPCLGPLH
                     TEFDGKGYAYTSCFVSSEAVKWKLGTWEVVQHLPAYY"
BASE COUNT          740 a          614 c          677 g          616 t
ORIGIN      
        1 acaataggaa gacaaaagag gttttgaaca tgacgccgca cgatgagact cgcgatgtat
       61 gatgtaaatc atgtttttgc ctgacgcgga agttggaaaa gtttttgact gattcgcgcg
      121 cgccgttctt ccgtaagcgc ggatggccag gtccgtccgg ataaatacaa aggaggagtc
      181 actatgagac ttcaatggat tacattgctt ctttccgtag cgttattcgc gactgcttgc
      241 aaagataaat caggtccagc aggcggcggg attgcgaacg tttcggatga ggaatcggcg
      301 aagacagttt tgcaaatcgc agccggttct aaagatcaca caactctggt gaaggcagtc
      361 caggctgcgg gattggaaaa ctctctcgcg aatcagggac cattcacagt tttcgcaccc
      421 acagacgcag cattcaaagc tctgccatcc ggtgcactcg acaaagtgat ggcagataag
      481 aatctgctca ccacagtttt gggatatcac gtctatgtcg gaagctctct caaagagtcc
      541 atgcttacag acggagatga gctttccatg ttcgatggcg gaactgcaaa aatcagcgta
      601 aaaggcggga aaatcagcat aaatggagcc aacgttctgg ctgctgtgcc cgcttcaaac
      661 ggcatggtat acgtgataga caaggttctt ctgccaccgg aaaagaagta aaatctttca
      721 ccttcgagtg agaaggcggg cgcggcccgc cttttttttt gcccgctttt tgcgtcttga
      781 tctgcgtcaa gtagcctctg aacaaatgtc atacagttct tatggcgtaa tcaacgccca
      841 ttcatttgac tctctgcgct cctttcggtt cctgaaatca aagggagaaa gtccccggga
      901 gttgaaatga tgaaccgtat gaaaataggc acagcactgg tgactgcact gttcctggct
      961 gttgcacttg cagcatgcaa gaagcaatca ggtgacaacg cgagcaatga ttctgggacc
     1021 ggaatcggtc ccgttagcag cgtacagatc gctgccggta tcgatgaagg catggcgaag
     1081 aagggagcgg atctcttcga ggcgaaatgc tccgcctgcc acaaatggga agagaaggtg
     1141 gttggtcccg cacttcaggg cgtaaccacc agaagaaaac cagaatggat catgaacatg
     1201 atcctcaatc cggttgaaat gactcagaaa gatccaactg ctcagaagct tctcgaagag
     1261 catctgacgc agatgacatt ccagaatgtt actcaggaag aagcgcgtat gattctggaa
     1321 catttccgta agaaagatgg cgcgaaatag gaggacgaca atgaaacgat tgcaaataat
     1381 tagcagcgta ttctttgctc tcgttgcatt cgtagcgtgt ggaaaggcag gtactgccgg
     1441 actcgcttcc gatgcagcag aaagagtata cgtaaagcca ggagacaaag acgaaatcta
     1501 tatgttcgct tccggcggat tcagcggcca ggtgggcgtg aatggaattc cgtccggtcg
     1561 tttgatccga gtgattccgg ttttttctca atggccggaa acaggatatg gatacgacga
     1621 aaccacgaag aacatgctga tgacgtcgca cggttttgtt ccatgggatg attcacatca
     1681 ccctcagctt tccgtaacag aaggaaagca ggacggaaga tggttgttca tcaacggaaa
     1741 taacactcct cgcattgcgc gcattgatct gaagtctttt gagaccgtag agatcattga
     1801 actgccaaac agcgcgggta accacccatc ttcattcctc actgagaatt cagagtatgc
     1861 tctggctgga acgcgattct caattccgat tccaaacgtg ccaatggcat gggaacaatt
     1921 ccagaaggaa atgaaagcag gcatgagctt cgtcagcatt gacaagacaa ccggaaagat
     1981 gtcaatcgac ttccaggttc tgcttccagg tatttcttat gacctcggaa gatgcggtaa
     2041 aggtccttca cacgactggt gcttcatttc ttcttacaac acagagcagg cttacaagat
     2101 gctcgaagtc aatgcttcca agaaagacaa agactttgta atggcgatca actggaaaaa
     2161 ggcaaaggcc tgcgtagacc agggcaaggc tcgtatgttc cctggagcat accgccacaa
     2221 ttacagagaa gagaatcagg aagctgtatc agaatcgctg aacggcacga agatgctcga
     2281 tccaaaggat tgccctggca tggtattcta catgccaaca cctaagagcc ctcacggttc
     2341 tgacgtggat ccaaccggtg aatacattgt tgcaggtgga aaactcgcca ctgtgattcc
     2401 agttcactct ttctccaaaa tgctcaaggc aatcgaagac aaagcgttca gcggcgaatt
     2461 ctttggaatt ccagttttga agtatgaagc tactctggcc ggtgaagttc agaaaccatg
     2521 cctcggtccc cttcatactg aattcgacgg aaaaggatac gcgtacactt cgtgtttcgt
     2581 ttcttcagaa gcagtgaagt ggaagcttgg aacatgggaa gtggttcagc accttccagc
     2641 atactat
//
LOCUS       DAOQJL010000544         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_27877_length_2625_cov_3.622179, whole genome shotgun sequence.
ACCESSION   DAOQJL010000544 DAOQJL010000000
VERSION     DAOQJL010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27877_length_2625_cov_3.622179"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="PLG78_17705"
     CDS             complement(<1..2066)
                     /locus_tag="PLG78_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE25013.1"
                     /translation="MPLKIFPETLAGDPVDLLRAGRWGELQRYFRSNRASSDTHRYIL
                     GRAMQEKIHKIPISPETAPLAESLREPVSIYLGLLGLTCDLSSQMKLNDCIHDLPNES
                     VTSQIPRLAILKLSAMLHATGWNDARLRLLSKSALREPDPLTAKIYTEKLEVLLAKQM
                     NAEALELAGRLGGVSQDKTHFYRARAYIRAGRRDDGWNLYLLAAARSLDDPGLLRSIF
                     QDLKYYFPAAFRAGSDQHSLHRSLFMFYDFLTPQEKAALASAWPAPRLLETTSPQTTL
                     GDGNHLISIGDRASFQDLAKRSYTAVSREPLILYKWGWKLTAARRYAETLSLIDQFDF
                     VKKENAGIWRLQLDTLEAMGDKDRAFRETLAYLAVFDYDLEVHDSLIEFLIGSDDKHI
                     HWAKPAYWELARGSMPPGSANGRFVYWLRRMYLATGETEKAKDVERKFYEMAPGSYYA
                     RAFWDEMQPGDFRRDWQDVRDRPTYLRWIGRHGGNPDAIRFLAARNLSSYFDPRATAL
                     WQRLQSVQYSVPEPVVTVWKLGEFDLGAEYFDYYFKDRMSRKELFLRKVMLGLKSGHL
                     DQSVWHLRQLARDENIPEDPFSIAPDFLKQLYPRPYLREVRSASSEFGLSEDMIYALM
                     RQESMFRELAVSRSGALGLMQVMPATGRWLAPRMQMKNPDMLEPSVSIRMGGKFFADL
                     LRSYDN"
     gene            2229..>2625
                     /locus_tag="PLG78_17710"
     CDS             2229..>2625
                     /locus_tag="PLG78_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25014.1"
                     /translation="MPAWQDASIHEEAARIIPLLYRSFVDGSVQEDTASMPFPNFTRQ
                     RLLRAVCGFAVWKGELDWLYSLALTAGATDPVIFLALSSALLEGRSTLASFFDGRLKQ
                     RMPLMDDLLHGIRVPYDEQEPDESAGFLNY"
BASE COUNT          608 a          648 c          710 g          659 t
ORIGIN      
        1 tcattgtcgt aggagcgaag aagatccgca aagaatttgc cacccatgcg aatggatacg
       61 gatggctcaa gcatgtccgg gttcttcatc tgcatcctgg gcgcaagcca cctgccagtg
      121 gcgggcatga cctgcatcag accgagcgct ccggatctgc tgactgccag ttcgcgaaac
      181 atggattcct gtctcatcag tgcgtagatc atgtcttcgg aaagaccgaa ctcggaggag
      241 gcggatctca cttcgcgcaa atacgggcga gggtagagct gcttgagaaa atccggcgcg
      301 atagaaaaag gatcttctgg aatgttttca tcccgtgcaa gctgacgcag gtgccataca
      361 gattgatcca gatgaccgga tttcaagcca agcatgacct ttctcaggaa gagttccttt
      421 ctggacatac gatctttgaa gtagtagtca aagtactccg cacccaggtc aaattctccg
      481 agcttccaga ctgtaactac aggctcgggc acggaatact gaacggattg cagtctttgc
      541 catagcgcag tcgctcgcgg atcaaagtaa ctcgataagt tgcgcgcagc caggaagcga
      601 atggcgtctg gatttcctcc gtgcctgcct atccagcgca agtatgtagg ccggtctctt
      661 acgtcctgcc agtcgcggcg aaagtcgcct ggctgcattt cgtcccagaa cgcacgagcg
      721 taatacgaac caggtgccat ttcataaaat ttcctttcta catcttttgc tttttcagtt
      781 tctccggtgg ccagatacat tctgcgaagc cagtaaacga atctaccatt cgcacttccg
      841 ggaggcatgc ttccgcgtgc gagttcccag taggcaggtt tggcccagtg aatgtgtttg
      901 tcgtcgctgc cgatcagaaa ttcgatcagg ctgtcgtgca cttccagatc gtaatcgaaa
      961 actgcgagat aggcaagagt ctctctgaat gcacggtctt tgtctcccat tgcttccaga
     1021 gtatccagct gcagtctcca tattcctgcg ttttcttttt tcacaaaatc aaattgatct
     1081 atgagggaaa gagtttccgc ataacgtctg gccgcggtca gtttccatcc ccatttgtac
     1141 aaaatcaagg gttcacgcga aacagcagtg tagcttctct ttgcaagatc ctgaaaagaa
     1201 gcgcgatctc caatcgagat gagatgattg ccatctccca gagtggtctg aggagatgtg
     1261 gtctcgagga gtctgggggc aggccaggcg gaagcgagtg ctgctttttc ctgtggagtc
     1321 aggaaatcat agaacatgaa tagagaacga tgaaggctgt gttgatccga tccagcgcga
     1381 aaggctgcgg gaaaatagta tttcaaatcc tggaagatgg acctgaggag tcccggatca
     1441 tcgaggcttc gagcagccgc aagcaaatac aaattccagc catcgtctct tcttcctgcg
     1501 cgaatgtagg ctctggcgcg atagaaatga gtcttgtcct gcgaaacacc acccagtcgt
     1561 ccagcaagct cgagtgcctc tgcattcatt tgtttggcaa gaagcacctc tagcttttcg
     1621 gtgtagatct tcgctgtgag tgggtccggt tctctgagcg cggatttgct cagtagcctg
     1681 agcctcgcgt cattccatcc agttgcgtgg agcattgcgg atagcttgag tatggcgagc
     1741 cgtggaatct gggacgtcac gctttcgttt ggaagatcgt ggatgcaatc attcaacttc
     1801 atctgactgg aaaggtcaca ggtaaggccc aggagtccga ggtagatcga aacaggttcc
     1861 cgaagagatt ctgcaagcgg cgcggtttcc gggctgatag gaatcttgtg tattttttcc
     1921 tgcatggccc ggccgagaat gtatctgtgg gtgtcggaag aagctcgatt ggaacggaag
     1981 taacgctgga gttctcccca gcggccggcg cgcaggagat ccacgggatc cccggccagg
     2041 gtttccggga aaattttcag tggcaaaacc aggatgagaa gcaggataga gcctggatgc
     2101 gaccggcgga agaatttttg gaaagattgg atcatgcaac ccatgattcg ttcttcttac
     2161 cgagttttct tggcccgggt atgctgggaa gaactgtttt ccaggcccat ccgtgcgttc
     2221 tatttcgaat gcccgcgtgg caagatgcat ccatacacga ggaagctgcc cgaatcattc
     2281 ctctattata tcggagcttc gtagatggct ccgtgcaaga agatacggcg agtatgccgt
     2341 ttcctaactt cactcgccag agattgcttc gcgctgtgtg cggctttgca gtatggaaag
     2401 gagaactgga ctggctgtat tctctcgctt tgactgcagg tgccacggat ccagtgattt
     2461 tccttgcact ctcttctgcg cttctcgaag gcaggtccac acttgccagt ttctttgatg
     2521 gcagattgaa gcaaagaatg cctttgatgg acgatttgct tcatggaatt cgcgtgcctt
     2581 atgatgagca agagccggat gaatccgctg gattcttaaa ctact
//
LOCUS       DAOQJL010000545         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28023_length_2618_cov_4.205618, whole genome shotgun sequence.
ACCESSION   DAOQJL010000545 DAOQJL010000000
VERSION     DAOQJL010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28023_length_2618_cov_4.205618"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLG78_17715"
     CDS             complement(<1..452)
                     /locus_tag="PLG78_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE25015.1"
                     /translation="MNNGRKTHPGKPIIYDMKILVTGATGLVGAEVLREAIKDRGIEQ
                     AIALTRRPGPVVDAKIHSITHTDFLDYSGVNLREVDAIIWCLGVSQSQVNKEQYEVIT
                     HDYAVAAGKALLLANPAGTFVFLSGTGADSSEQTNTLFGRVKGKTENSL"
     gene            498..2417
                     /locus_tag="PLG78_17720"
     CDS             498..2417
                     /locus_tag="PLG78_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE25016.1"
                     /translation="MPLFEKISPASPALSEFVASIQGLQMHKGGIVYRQGTLPQSIYL
                     VRDGEIRITAEDNGQKKLIGMQSRGSLFGEVSFLTGESHSSSAEASLDSTLLVIPGPA
                     FFKLLSQESSVGEGLARLLSQRLRNRMRPESSTDSTTAVAVLYPDSPRNGSELCAELG
                     EAMLETGADQILILSWNPRGVFADRGAGDLRRLIRSWPDLRPEGEDGYVTVYHAALEG
                     EDKNKLCSILAGLIALLKKYYAAVIIDPGIDHSDSLVREALEQADRVVLVQSCRDPSV
                     LRSKVFAETISSCVKADSDFFSRAILVTHESPGEGALPHIVEGRFERHLRLRGVSERI
                     EETDRTTRSGVRRIARALLGVSRALCLGGGGARAFAHIGVLEVFEENGIEFDAVAGTS
                     MGAVMAGADALGLSAEEIEYNVRRILPHSSAILDKTIPLVSFFTGRNLNRTILRFFGD
                     LKFEDLSLPFFCNAVDLNSGKEIVFEKGYLAAAIRASVSIPGVFPPFRHGPYALVDGA
                     VLNNLPGDILKSRGYDIILGVNVSPLEDHRPVSFGIQTRKGWVRGIRDYFSIPPILRI
                     INRSIAIESRQLLKIHMEQFEYLLHPEVSDFDTFDFERKKEIIDAGRTAARTHIGEIK
                     DTFTRQSLGRREHER"
     gene            complement(2421..>2618)
                     /locus_tag="PLG78_17725"
     CDS             complement(2421..>2618)
                     /locus_tag="PLG78_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family protein"
                     /protein_id="HNE25017.1"
                     /translation="RFTMYPDSVQGGNGGFYTKERVVRNAHVTTSLAAGSAFEFALDI
                     VEQLLGPEKKAAVAGPMFLPQ"
BASE COUNT          611 a          656 c          688 g          663 t
ORIGIN      
        1 agcgaattct ccgtttttcc cttcactctg ccgaataacg tgttcgtttg ttccgaggaa
       61 tcggcacccg tgccgctgag aaacacgaac gtcccggctg gattggctag aaggagcgcc
      121 ttacccgcag caactgcata gtcatgagta atgacttcgt actgttcctt gtttacctgg
      181 gactgggaca cacccaggca ccagatgatc gcgtccactt ctcggagatt cactccactg
      241 taatcgagga aatcagtgtg ggtgatggaa tgaatctttg catccacaac cggtccgggc
      301 cggcgagtca gtgcgattgc ctgttcgatt cctctgtctt tgatcgcttc gcgcaatact
      361 tccgcgccca cgaggcctgt agctccagtc accagtatct tcatatcata gataatcggc
      421 ttaccgggat gagtttttct gccgttattc aaaaggtgcc cacagatccc gaaagtatcc
      481 ggcggttttt gcgcggtgtg ccgctcttcg aaaagatttc cccggccagc ccggctctct
      541 ctgagtttgt agcgtcgatt caagggctac agatgcacaa aggggggatc gtataccgtc
      601 agggcacttt gcctcagtcc atctatctcg tgcgcgacgg agagattcgg atcacagcgg
      661 aagacaatgg acaaaagaag ctcattggaa tgcagagcag gggaagcctg tttggagagg
      721 tcagctttct aacaggcgaa tcgcattcat cgagcgctga ggcctctctg gattctacct
      781 tgcttgtgat tcctggtcct gcctttttca aacttctttc tcaagagagt tccgtcggag
      841 aaggtctggc aagacttctt tcccagcgtc ttcgcaatcg aatgcgcccc gagtcatcaa
      901 ctgactcgac cactgctgtg gccgttcttt atccagacag cccgcgaaac ggaagcgagc
      961 tttgcgccga gcttggagag gccatgctcg aaacaggagc ggaccagatt ctcattctat
     1021 cctggaatcc tcgcggagta ttcgcagatc gcggtgccgg cgatcttcgc cggttgattc
     1081 gatcctggcc cgatttgagg ccggaaggcg aggatggata cgttactgtc tatcacgcgg
     1141 cactcgaagg cgaagataaa aacaaattgt gcagcatcct ggcaggcttg atcgctttgc
     1201 tcaagaaata ctatgccgcc gtaatcatag atccaggtat agaccactct gattcgctcg
     1261 taagagaagc actcgagcag gcagatcgtg ttgttctagt ccaatcgtgt cgtgacccct
     1321 ctgttcttcg cagcaaggta tttgctgaaa ctatttcgag ctgcgtaaaa gcagactccg
     1381 atttcttctc ccgggccatt ctggtgacac atgaatctcc gggggaagga gcattgccgc
     1441 atatcgtgga aggaagattc gagagacatc ttcgccttcg cggagtaagc gaaagaattg
     1501 aggaaacaga tagaaccacg cgcagcggcg tacgacgaat tgctcgcgca ctgctcggag
     1561 tatcaagagc tctctgtctg gggggtggag gtgcgcgggc atttgcacat attggagtcc
     1621 ttgaagtatt cgaagaaaac ggaattgagt ttgatgctgt ggcgggcact tccatgggcg
     1681 cagtcatggc cggagcggat gctcttggtt tgagtgcgga agagattgaa tataacgtca
     1741 ggcgaatatt gcctcactct tctgcgattc tggacaaaac gattcctctc gtaagtttct
     1801 tcacgggcag aaacttgaac cggaccatcc tcaggttttt cggagacttg aaatttgaag
     1861 atctcagttt gccgtttttc tgcaatgcgg tggatctgaa ttcaggcaaa gagatagttt
     1921 ttgaaaaagg gtatctcgcg gcggccattc gcgcaagcgt cagtatcccg ggagtgtttc
     1981 ctcctttcag acatgggccc tacgctctcg tagatggtgc tgttttaaac aatctgcctg
     2041 gagacattct caaatccaga ggatacgata ttatcctggg agtgaatgtt tcccctctcg
     2101 aagaccatcg tcctgttagt tttggaatcc agactagaaa gggatgggtg cgtgggatca
     2161 gagattactt ttctattcca cccattttgc gcatcatcaa ccgatcgatc gccattgaaa
     2221 gccgtcagct attgaagatt catatggagc agtttgagta tttgcttcat ccagaggttt
     2281 cagactttga tacctttgat tttgaacgaa aaaaagaaat cattgatgcg ggtcgaaccg
     2341 ccgcgcgcac acacatcgga gagatcaaag atacatttac ccggcagagc ctggggcggc
     2401 gcgaacacga acgttaggcg ctattgcggt agaaacattg gcccggccac ggcagccttc
     2461 ttctctggac caagcaattg ttccacaatg tccaatgcaa attcgaatgc tgaaccggcc
     2521 gcgaggctgg ttgtaacatg cgcgtttcgc acgactcttt cctttgtgta aaatccgcca
     2581 tttccgccct gaactgaatc tggatacatg gtaaagcg
//
LOCUS       DAOQJL010000546         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28197_length_2610_cov_3.334638, whole genome shotgun sequence.
ACCESSION   DAOQJL010000546 DAOQJL010000000
VERSION     DAOQJL010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28197_length_2610_cov_3.334638"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLG78_17730"
     CDS             <1..348
                     /locus_tag="PLG78_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNE25018.1"
                     /translation="MKTEKIDLVMVGADRIAHNGDTANKIGTLGLAVLAQHFGIPFYV
                     VAPSTSVDPNLASGDLIHIEERSAAEVRTIAGISTSPEHIAVRNPAFDVTPAELIRAI
                     VTEKQIHFPPYSF"
     gene            complement(429..>2610)
                     /locus_tag="PLG78_17735"
     CDS             complement(429..>2610)
                     /locus_tag="PLG78_17735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766994.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNE25019.1"
                     /translation="WSCADPGIQYDTTINEWHTCPEDGRINASNPCSEYMFLDDTACN
                     LASVNLLTLYNKDTGEFDDLGYRHTARLWTLVLEISVTMAQYPSQEIARLSHVFRTLG
                     LGYANLGSLLMVMGHSYDSEKARAIAGALTAILHMSAYATSAEISERMGPFEGYERNA
                     KNMLRVIRNHRRAAYNAPASEYEGLSVFPVGISPVHCPDYLLKAAREESDRALALGEK
                     HGYRNAQVTVLAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINQSIPPALK
                     KLGYNAEQADAIVKYCVGHGTLKGAPEINPETLKAKGFTDEALARIDEALPSAFELGF
                     LMNRFTLGLDFIKNTLGFGDDAEKPDFDLLTRLGFNKEQVRRANDYACGTMTIEGAPH
                     LAEEHYAVFDCANKCGRYGKRYLSVESHIRMMAAAQPFISGAISKTINMPQEATVSDV
                     KRAYRQSWELMNKAIALYRDGSKLSQPLNVQSDEEEDHSSTPHVQKVEKIVEKIVYRT
                     LTSRRRLPERRAGYTQKATVGGHKIYLRTGEYEDGHLGEIFLDMHKEGAAFRSLMNSF
                     AIAISLGLQYGVPLEEFVEAFVFTRFEPNGMVQGNRAIKMSTSIIDYVFRELAITYLD
                     RHDLAQVSPEDLRADSVGMGTEEGDSGKKTSEAADFDLPAFIGGREPSNSRASAAVAE
                     PSLGQLARLKGYEGDPCPECGQFTLVRNGSCLKCNSCGSTTGCS"
BASE COUNT          599 a          672 c          658 g          681 t
ORIGIN      
        1 atgaagacag agaagatcga tctggtcatg gtgggtgcag atagaattgc gcataacgga
       61 gatacggcga acaagattgg aacccttggc cttgcggttc tggcgcagca ttttggaatt
      121 cctttttacg tggtggctcc gtctacttcc gtagatccga atcttgcctc aggcgattta
      181 atacacattg aagaacgatc tgccgcggaa gttcgaacga tagccggcat ttcaacgagt
      241 ccagagcaca ttgcagttcg caatccggcg tttgacgtca cgcctgcgga actgatccgc
      301 gccatcgtca ctgaaaaaca aatccatttc ccaccgtact ctttctaggc tatcactgac
      361 ccggcgcgaa ggcataaaaa aacccggccc cgatgcgagg ccaggttttc gcgtgattcc
      421 cgcctatatc aggaacagcc tgtggtggag ccgcaggagt tgcatttcag acaacttccg
      481 tttctcacca gggtgaactg tccgcactca gggcatggat cgccttcgta gcctttcagc
      541 cttgcgagtt gtccaagact tggttctgcc acggcggcgg atgccctgct gttagacggc
      601 tcacggccgc cgatgaaagc gggcaggtca aaatccgcgg cctcactcgt cttttttccg
      661 gaatcgcctt cttctgttcc cattccgacc gaatctgcgc gaagatcctc cggagaaacc
      721 tgagccagat catgtctgtc cagatatgtg atggcaagtt cgcggaaaac gtagtcaatg
      781 atggacgtag acatcttgat cgcgcggttt ccctgcacca ttccatttgg ttcaaagcga
      841 gtgaacacaa acgcttctac gaattcttcg agaggcaccc cgtactgcag acccagagaa
      901 atggcgattg caaacgagtt catcaatgaa cggaatgctg cgccctcttt gtgcatatcc
      961 aggaatattt ctcccaggtg accgtcttcg tattctccag tgcgaagata gatcttgtgg
     1021 ccacccacag ttgctttttg ggtgtagccc gctcttcttt ctggaaggcg tcttctgctt
     1081 gtgagagtgc gatatacaat cttctccaca atcttttcta ctttctgaac gtgaggagta
     1141 gaagagtgat cttcttcctc atcgctttga acgttgagag gttgagaaag tttggatccg
     1201 tctctataca gagcaattgc tttgttcatc aattcccagg attgacgata ggcgcgcttg
     1261 acgtccgata ccgttgcttc ctgaggcatg ttgattgttt tggaaatggc accggaaatg
     1321 aaaggctggg ctgcagccat cattctgatg tgagattcta cagagagata tctttttcca
     1381 taccgaccac acttgttggc gcaatcaaac actgcatagt gttcctctgc aagatgagga
     1441 gcgccttcga tggtcatcgt gccgcatgcg tagtcattgg cacgacgcac ttgctctttg
     1501 ttgaatccca gcctggtcag cagatcaaaa tcgggtttct ccgcatcatc accaaagccc
     1561 agggtattct taatgaaatc caggcccaga gtgaagcgat tcatcaagaa tcctagttca
     1621 aacgcggatg gaagtgcctc atcgattctg gccagtgctt cgtctgtgaa tccctttgct
     1681 ttgagagttt caggattgat ttcaggcgca cctttcagtg ttccgtgtcc cacgcaatac
     1741 ttcacgatgg catctgcttg ctctgcattg tatccaagtt tcttgagggc aggtggaatg
     1801 ctctggttga tgatcttgaa gtacccaccg cctgcgagtt tcttgaactt cacaagtgca
     1861 aagtcaggtt caatccctgt ggtgtcgcaa tccatgacca ggccaatggt tccggttgga
     1921 gcaagcacag taacttgcgc gttgcgatac ccgtgctttt ctcccagagc cagagcacga
     1981 tcggattctt cgcgcgccgc tttcagaaga tagtccgggc agtgcacggg tgaaattcca
     2041 actggaaaca cgctcaatcc ttcgtattcg gaagcaggag cgttgtaggc agcacggcga
     2101 tggttgcgaa tcactcgaag catgttcttc gcattgcgct cgtatccttc aaaaggcccc
     2161 attctttctg agatttcagc actggttgca tacgcactca tgtgcagaat ggcagtcaaa
     2221 gcgcctgcaa tcgcgcgcgc tttttctgaa tcataagaat gacccatcac catcagcaga
     2281 cttcccaggt tcgcatagcc aagacccaga gtgcgaaata cgtgagagag tctggcgatt
     2341 tcctgggatg gatactgcgc catcgtcact gaaatctcca gtaccagagt ccacagtctc
     2401 gcagtgtggc gatagccaag atcgtcgaat tctcccgtgt ctttgttgta aagagtcagc
     2461 aggtttacgg atgcgagatt gcatgcagtg tcatccagga acatgtattc cgaacatgga
     2521 ttggatgcat tgatgcggcc atcttccggg caggtatgcc attcgttgat ggttgtgtca
     2581 tattgaattc caggatctgc gcaggaccag
//
LOCUS       DAOQJL010000547         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28267_length_2606_cov_4.121521, whole genome shotgun sequence.
ACCESSION   DAOQJL010000547 DAOQJL010000000
VERSION     DAOQJL010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28267_length_2606_cov_4.121521"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLG78_17740"
     CDS             complement(<1..467)
                     /locus_tag="PLG78_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25020.1"
                     /translation="MSFPVSISLSQAGISFAAIAWMAGIVARSDVPRFPWPRLLIAGL
                     CIYGLELLSLILLSFSGGGFAFIKQGLSREIKDVFLILMAFWVFAFSEDEDRRKTIEK
                     LTYIAIGILIVSGLIGIFSRYRLSNIPYHLMHGWEGNMQARYQHHAGTFLAGTP"
     gene            569..1240
                     /locus_tag="PLG78_17745"
     CDS             569..1240
                     /locus_tag="PLG78_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25021.1"
                     /translation="MERRLVTGLALAVFVASGLFLLYKVRSEEQDFLGYQGRTAISTN
                     SIFDPALSPELAIDSDRKTFWKESMPGVQGTCTNETRTAPESDTRPPCLFLLIDAGFS
                     HRPGRPPEPDMPVSLTLEPGPAEYARPRAVRVVFFDQEIVDMDREFRLPTSPVFLAEK
                     SFVLSDGINHLPLDFLPPARVTAVYPGSVRKLWVRVEIESVYPGSKYSNTVGISELFV
                     ERKKQ"
     gene            1237..>2606
                     /locus_tag="PLG78_17750"
     CDS             1237..>2606
                     /locus_tag="PLG78_17750"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNE25022.1"
                     /translation="MSAAEVFRILKRTWERFNEDGCYYRASAIAYALVLSAIPLMTIL
                     VKFAQVDQETIRAYLSRFMGAYGIQDSSEFLHALDEILSRADSIALVGVIFMIISATS
                     ILRHLEDSFNHIARSHVERPLHYRFAFYISGLILVPAFTTVTAGTMQYFLSQLKPPEW
                     KSMTLNDDTLWMLGKKLAWKQAKGEGSFHLETKIDKHAPYKDVYFDLQTGRSGRSWEI
                     LGEVPPRSLEAKDIRLLSRIDSADGVLYILGETGAVYFSNDNGQTFDYRLFAFKGASG
                     LRRPIFEDLLALPGKRALVLATVGSRSCLVDFKENAYGMQCFSTVYNNIVQIPNGKKS
                     EIYITGTARVLRSDDGGVTWNGPFDERYGSRTLMINTMARDGKGQTFFGGGSLWIRDQ
                     KGQLFLPELRAEHDIIGMHIDKDGQGFVYGDDELFRYTNDAGRTWYAPSNRILKNTTF
                     YSHQVLP"
BASE COUNT          637 a          640 c          662 g          667 t
ORIGIN      
        1 ggagtgcccg caaggaatgt tcccgcatga tgctggtatc ttgcctgcat gttgccttcc
       61 cagccatgca tcaaatgata aggaatgttg ctgagcctgt atctgctgaa gatgccaatg
      121 agtcccgaaa cgatcaggat tccaatcgca atgtatgtta gtttttcaat ggtctttcgc
      181 cgatcttcgt cttctgaaaa agcgaaaacc cagaatgcca tcaagatgag aaatacgtct
      241 ttgatttcgc gcgaaagccc ttgcttgatg aatgcaaatc cgcctcccga aaaggaaaga
      301 aggatcagag acaacagttc gagaccatag atgcagagcc ctgcgatcaa aagacgcggc
      361 catgggaaac gcggaacatc cgaacgagcc acaattcctg ccatccaggc gatcgccgcg
      421 aagctgattc cggcctggga aaggctgatg gagacaggaa agctcacaac ggatactgct
      481 ccgcagtacc aggcgattcg gtaaaaaaga cttcgatctt tcaatattgt ttgcgtctga
      541 atcaatcagg ggaactgtca gcaaaaatat ggaacgcaga ttggtcaccg gtttggcgct
      601 cgctgtgttt gttgcctcgg ggttgtttct tctatacaaa gtgcgcagcg aggaacagga
      661 tttcctgggc tatcaggggc gcactgccat tagcaccaat tctatctttg atcctgctct
      721 gagtccagag cttgccattg attctgacag gaagactttc tggaaggagt ctatgccagg
      781 agtccagggc acctgcacga atgaaacgcg cacagctcct gaatctgaca cgaggccgcc
      841 gtgcttgttt cttctgatcg atgctggatt ttcgcacaga cccgggcgtc ctcctgagcc
      901 agatatgccg gtttctttga ctctcgaacc aggccccgcc gaatatgctc gcccacgcgc
      961 agtgagggtc gtgttctttg atcaagaaat cgtggatatg gaccgcgaat tcaggcttcc
     1021 cacatctccc gttttcctgg cagagaaatc tttcgttctt tcggatggaa tcaatcattt
     1081 gcctctggat tttctgccgc ccgcgcgcgt gactgcagtg tatccggggt ctgtgcgaaa
     1141 gctctgggtt cgcgttgaaa tagaatctgt gtatcccggt tctaagtatt caaatacagt
     1201 tggtatttct gaactatttg tggagagaaa gaaacaatga gcgcggctga agtctttcgc
     1261 attttgaagc gaacctggga gcgcttcaac gaagacggct gctactatcg cgcgagtgcg
     1321 attgcctatg ctctggttct atctgcgatt ccactgatga ccatccttgt gaaatttgcg
     1381 caggtggatc aagaaaccat ccgtgcgtat ctgtcgcgat ttatgggagc gtatggaatt
     1441 caggattcat ctgaattctt gcacgctctg gatgagatcc tgagccgcgc ggacagcatc
     1501 gcgctcgttg gtgtgatctt catgatcatt tccgccacaa gcattttgcg gcatctggaa
     1561 gattcattca atcacattgc tcgttcccac gtagagcgtc cgctgcacta tagatttgct
     1621 ttttatattt ccgggctcat tcttgttcct gcatttacaa cagtgactgc cgggaccatg
     1681 caatactttc tcagccagtt aaagccaccc gaatggaaat ccatgacgtt aaacgacgat
     1741 actctgtgga tgcttggcaa gaaactcgca tggaaacagg caaaggggga aggatcgttt
     1801 catcttgaga caaagattga taaacacgca ccttacaagg atgtctattt cgatctgcag
     1861 acgggccgct ctggaagatc ctgggaaatt ctgggggaag tgccgcctcg ctctctagag
     1921 gcaaaagaca ttcgtcttct ttctcgcatt gactcagcag acggcgtact ctacatactg
     1981 ggagaaaccg gagcagttta tttttcgaac gacaatggcc agacgtttga ctacagactc
     2041 ttcgcattca agggcgcaag cgggctcagg cgtcctattt tcgaggatct acttgctttg
     2101 cccggaaaac gagcgctcgt tcttgccaca gtcggttctc gttcttgcct cgttgatttc
     2161 aaagagaatg catacggcat gcagtgcttt tctaccgtct ataacaacat cgtccaaatc
     2221 ccgaatggaa agaagagcga aatatacatc actggaacag ctcgcgtact gcgctcagac
     2281 gacggcggag ttacgtggaa cgggcccttt gacgaaagat atggttcgcg tactttgatg
     2341 atcaacacaa tggcgcgtga cgggaaaggg cagacttttt ttggaggcgg atctctctgg
     2401 attcgcgatc agaaaggaca gctctttctt ccggagcttc gcgcggaaca tgacatcatc
     2461 gggatgcaca tagacaaaga tgggcagggt tttgtgtacg gagacgatga attgtttcgc
     2521 tacaccaatg atgcgggccg cacctggtat gctccttcca accgaatctt aaagaatact
     2581 accttttatt cgcaccaggt tttacc
//
LOCUS       DAOQJL010000548         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28336_length_2602_cov_4.970161, whole genome shotgun sequence.
ACCESSION   DAOQJL010000548 DAOQJL010000000
VERSION     DAOQJL010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28336_length_2602_cov_4.970161"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLG78_17755"
     CDS             <1..187
                     /locus_tag="PLG78_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HNE25023.1"
                     /translation="EEDKGTSPLALSRVNLNDQTVEGFGDPARKILSIQYHPEACPGP
                     HDAEYLFAKFYEMVKGK"
     gene            184..1221
                     /locus_tag="PLG78_17760"
     CDS             184..1221
                     /locus_tag="PLG78_17760"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNE25024.1"
                     /translation="MKKIAFLLICLLAACHTGDKKDPAKTEPSQPAKPETAKPPGQPA
                     IAVKPGTRPGEVLNRIRFIVGEEAITDMDIEAMRKNLLRMGKPVRNLQEDAVKELIRR
                     ALVENEAKAESIIVSEQRVENEVTRAKENAGVRDDARFRQGIEAETGMPYSLWLENLR
                     YDLIKQQLIQIKISVPPPTQEELRRFYGQNAARSGQEVRYREIVLQPQNQSIEEEARI
                     SNLARQIVQRSGGNATVFGELARTTPDNVSPLKMYSGMQDYISLTEVANFDRISAGVL
                     STLGPGQTSIPFRDARGRYVVFLMEGRRPVSFEKIEDRIRQKLFFDKAEQAFEEWIEK
                     KRKETAITAID"
     gene            1224..>2602
                     /locus_tag="PLG78_17765"
     CDS             1224..>2602
                     /locus_tag="PLG78_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HNE25025.1"
                     /translation="MPGKIRAVVIYAPSPDREMADATLAKIKEWMPGCNIYDNFDGTI
                     QVTGSSPFEMAKAAYSELARTTPVSPDDSLALFTGLCPFLDKGIADDLAKVHTQFYAH
                     YSYCENSPYGFLPDLVSSEFLAEAEPGATDLRDFVFKNIEKYDAEILYRRPDVRQFRL
                     DFSCGSPRSLDLSRRLRAIDPDVTYERIEKVIQDHPEIIRSFPSYFEIDLTSLSKVHP
                     ILYPEISGTDSLPLALAIKLNAEIAAHGWKKDATVSLAGRGEPMLHPDFLEILKLFLA
                     NESVSTVFVETYGHSIDEKQITLWNSLPGAEKLSVILRLSTLKKDRYRFLYGEDLFEP
                     AMAAVVHLEKAANRKFAAYVEMLKIKEVEDEITPFFDRFENSTVRVILNKYNRYIDLL
                     PERRVTDLTPLDRSFCWHIARDFYLKPDGTVPLCKQDIVGRAATHDFAKKTILEILSA
                     TARAHSHSV"
BASE COUNT          698 a          625 c          650 g          629 t
ORIGIN      
        1 ggaagaggac aaaggtacaa gtccgcttgc actttcgcgc gtaaatctga atgatcagac
       61 tgtggaagga ttcggggatc ctgcaaggaa gatactgagc attcagtatc atccggaagc
      121 gtgccccggc ccgcatgatg cggagtattt attcgcaaaa ttttatgaaa tggtgaaagg
      181 aaagtgaaga agatcgcctt tcttttgatt tgccttctgg cggcctgcca caccggagac
      241 aagaaagatc cggcaaaaac ggaaccttct caacctgcaa aacctgaaac tgcaaagcca
      301 ccgggccagc ctgcaatagc agtgaagccg ggcaccaggc ctggagaagt tctcaaccgt
      361 attcgcttca tcgtgggaga agaagccatc acagacatgg acattgaggc catgcgaaag
      421 aacctgctgc gcatgggtaa gcctgtgcgc aatctccagg aagatgccgt caaagaattg
      481 atccggcgtg ctctcgttga aaacgaagcg aaggcagaat cgatcatcgt atcagaacag
      541 cgcgtagaaa atgaagtaac tcgcgcaaaa gaaaacgcag gtgtgcgcga tgacgcccgc
      601 tttcgtcagg gaattgaagc ggaaacagga atgccgtact ctctctggct tgaaaacctt
      661 agatatgatc tgatcaaaca gcaactcatt caaattaaaa tatcagtgcc tcctcccacg
      721 caagaagagc tgcgtcgttt ttacggacag aatgcggcca ggtccggtca ggaagtgcgt
      781 taccgcgaaa ttgtactgca accacagaat caatctattg aagaagaagc tcgcatttcc
      841 aatctcgcaa gacagattgt tcagcgcagt ggtggcaacg caactgtgtt tggagaactg
      901 gcccgcacaa cgccggataa cgtatcgcct ctgaagatgt attctggcat gcaggattat
      961 atttcgctca cagaagtcgc gaactttgat cgcatcagcg caggtgtttt gtccacactc
     1021 gggcccggtc aaacgtcgat tccgtttcgc gatgcgcgcg gccgctatgt tgttttcctg
     1081 atggaaggca gacgccccgt ttcttttgag aaaattgaag atcgaatccg tcagaaactg
     1141 tttttcgata aagcagaaca ggccttcgaa gaatggattg aaaagaagcg caaagaaact
     1201 gcgatcacgg cgattgactg aagatgcccg ggaaaattcg cgcagtcgtt atatatgctc
     1261 cctctcccga ccgggaaatg gcggatgcaa ctctcgcaaa aataaaggaa tggatgccag
     1321 gttgcaacat ctatgacaac ttcgacggaa ccattcaggt aacaggaagc agtccttttg
     1381 aaatggcaaa ggccgcctat tctgaactgg cgcgcacaac tccggtgagc ccggacgata
     1441 gcctcgctct ctttacgggt ctgtgtcctt ttctcgacaa aggcattgcg gacgatctgg
     1501 ccaaagtgca cactcagttt tacgcgcact attcgtattg cgaaaacagt ccttatggat
     1561 ttttgccgga tctggtttca tctgagtttc tggcagaggc agagcccggc gctacggatc
     1621 tgcgcgattt tgtttttaag aacatagaaa aatacgatgc agaaattcta taccgccggc
     1681 cggatgttcg tcagtttcgt ctggattttt cgtgcggaag ccccaggtcc ctcgatcttt
     1741 cgcgtcgcct gcgagccatt gacccggatg taacatacga aaggatcgag aaggtgatcc
     1801 aggatcatcc tgaaatcatt cgttcatttc catcatattt tgagattgat ctgaccagcc
     1861 tgagcaaggt tcatcccatc ctttaccccg aaatctcagg tactgattct ttgcctctgg
     1921 ctctggcgat taagctcaat gcagagattg cagctcatgg ctggaaaaag gacgcaactg
     1981 tgtctcttgc gggtcgcggc gagcccatgc tccatcctga ttttttggaa atcctgaagt
     2041 tgtttttggc caatgaaagt gtcagcacag tctttgtgga aacgtacggt cattccattg
     2101 atgaaaagca gataacgtta tggaattcgc tgcccggagc ggaaaaacta tctgtcatac
     2161 tgcgcctttc cactctgaaa aaagatcgat acaggtttct ctatggagaa gatttgttcg
     2221 agcctgcgat ggctgctgtg gttcatcttg aaaaagcggc caatcgcaaa ttcgcagcct
     2281 atgtggaaat gcttaagatc aaggaagtgg aagatgagat cactcctttc tttgatcgct
     2341 ttgaaaactc gaccgttcgc gtaatcttaa ataaatacaa ccgttacata gatcttttgc
     2401 cggagcgcag agtcacggat ctcactcctc tggacagaag tttctgctgg cacatcgcgc
     2461 gtgatttcta cttaaagccg gacggaactg tgcctctgtg caaacaggac attgttggcc
     2521 gcgctgcaac ccatgatttt gcaaagaaaa cgattcttga aatcttatct gccacggcca
     2581 gggcacattc gcattctgta ag
//
LOCUS       DAOQJL010000549         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28396_length_2599_cov_4.589623, whole genome shotgun sequence.
ACCESSION   DAOQJL010000549 DAOQJL010000000
VERSION     DAOQJL010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28396_length_2599_cov_4.589623"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLG78_17770"
     CDS             <1..412
                     /locus_tag="PLG78_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE25026.1"
                     /translation="VSDGTCVRLAFDASIPGSMHGLLVGFISGENAREWSDRTPRERE
                     AAVVSDFVRFFGEEASDHRGYLEQNWIAEEFSRGCYAGLMPPSVMTSFGNALRDPCGL
                     IHWAGTETARHWNGYMEGALEAGERAAAEVLEAR"
     gene            complement(569..1033)
                     /locus_tag="PLG78_17775"
     CDS             complement(569..1033)
                     /locus_tag="PLG78_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25027.1"
                     /translation="MMYREPDFYERISALNKVKLQRLISWLSEKELVALMNSADLELE
                     DAVLDNTSHAGRIVVLENLGFSEEDFLKRASVRKKISDLYAEQILNMETPPDKSQRAA
                     IVDGIVANLNEEERAAYNNRFKEKVVEIGEALRHKLSGYLDLLEKGASYKHM"
     gene            complement(1030..1803)
                     /locus_tag="PLG78_17780"
     CDS             complement(1030..1803)
                     /locus_tag="PLG78_17780"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNE25028.1"
                     /translation="MEASSQKQANTDGKIFLTADWRHLLLVNYEVNPDILAPHVPRGV
                     ELDLFGGKCFVSLVGFMFLRTRLLGKLPIPFHTNFEEINLRFYVKRKEGEETRRGVCF
                     LKEIVPRYAIALVARKAYGERYVSHPMSHILDIKNSALAVNGRVVYSWLDEKRHSIGA
                     KCTGNPSYPAAGSQEHFIIEHYFGYVKSPAGQTVEYRVEHPEWRVLSCKEPRVDVDVA
                     RTYGPEFVEPLSKTPASVFVAEGSPVTVRQGRILPEAAG"
     gene            complement(1812..2207)
                     /locus_tag="PLG78_17785"
     CDS             complement(1812..2207)
                     /locus_tag="PLG78_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNE25029.1"
                     /translation="MIEVEEGKTLSLDFEKTRKAADAGAVLPVIVQDAKTKDVLILGY
                     VNDAALKQSIETGFVTFFSTSRKEIWVKGDTSGNRLRLKEIRVNCEQNSLLFLAELEG
                     QGACHTKEKDKYRYSCYYRRLEQGSLKFV"
     gene            2289..>2599
                     /locus_tag="PLG78_17790"
     CDS             2289..>2599
                     /locus_tag="PLG78_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25030.1"
                     /translation="MQELLLKRHSVFRGDVTLPGSKSIANRALLLSSLAQGKTILRGL
                     PDADDVTVFRRNLPNLGIQATEMGDSLEIVGAGGSFPVQKADIFVENAGTAMRPLAAA
                     L"
BASE COUNT          594 a          652 c          651 g          702 t
ORIGIN      
        1 ggtgagcgac ggaacatgcg ttcgccttgc atttgatgcg agcattccgg gatctatgca
       61 tggacttctg gttggattca tttccgggga aaacgcgaga gagtggtcgg accggacccc
      121 gagagagaga gaagctgccg tcgtgtctga ttttgttcgc ttctttggag aagaggcgag
      181 cgatcacaga ggatacctgg aacaaaactg gattgcagaa gaattcagtc ggggctgcta
      241 cgccggcctt atgcctcctt ctgtgatgac gtcattcggc aatgctctgc gagatccgtg
      301 cggactcatc cactgggcag gcacagaaac agcgcggcac tggaatggat atatggaagg
      361 agcactcgag gcaggagagc gcgccgccgc agaagtactc gaggcgcgct gattgcagac
      421 taagccgcgg ctttttgctg actttgatcg atgacgatcg ttcccgacca gcgatcatgc
      481 agagtgcgat gttgcgggga aaatagaaag aatacgtcta gaaagtatgt aaagccaaac
      541 ctattttgta accaacgaca caatcgcctt acatgtgttt gtagctggcg cctttttcga
      601 gtagatccag ataaccgctg agcttgtgcc tgagcgcttc tcctatctcc acaacctttt
      661 ctttgaatcg attgttgtaa gctgcccgtt cctcctcatt cagattggca acaatcccat
      721 ccacaatcgc ggcgcgctgt gatttatccg gaggtgtttc catattcaga atttgttctg
      781 catacagatc cgagattttt tttcgcacac ttgcgcgttt gaggaaatct tcctcggaga
      841 atccaagatt ctcgagtacc acgattctac cggcatgaga tgtattgtcc agcactgcgt
      901 cttccagctc tagatccgct gaattcatca gtgcgaccag ttccttttcg gaaagccagc
      961 tgatgagacg ttgcagtttg actttgttca acgcggatat tctttcatag aaatcaggtt
     1021 cgcgatacat catccggcgg cctccggaag gattcgtccc tggcgtactg tgaccgggga
     1081 accttctgct acgaaaacag aggcgggcgt ttttgacaga ggttctacaa attccggtcc
     1141 gtacgtgcgt gcaacatcta cgtctactct gggttctttg caggaaagaa cgcgccattc
     1201 tggatgttca actctatact caaccgtttg accggccggg cttttcacat atccaaagta
     1261 atgttcaatg ataaaatgtt cctgcgatcc cgcagccggg taggaagggt tccctgtgca
     1321 tttcgcacca atcgaatgcc tcttttcgtc taaccatgag tagacgactc tgccgttcac
     1381 tgcaagcgcg ctatttttaa tatccagaat gtgactcatg ggatgtgaca cgtatcgctc
     1441 tccatatgct tttcgagcaa ccagcgcaat tgcgtatctg gggacaattt ctttgaggaa
     1501 gcagactcct ctcctggttt cctctccttc ttttcttttc acgtagaatc taagattgat
     1561 ttcttcaaag ttggtgtgaa atggaatggg cagttttccc agaaggcgag tgcgaagaaa
     1621 cataaacccg accagactca cgaagcattt tccgccgaat agatccagct ctactccgcg
     1681 tggaacgtgg ggtgccagta tgtccggatt cacttcatag ttcacaagca ataaatgacg
     1741 ccagtctgcc gtcaggaaaa ttttgccatc cgtgtttgct tgcttttgcg atgatgcttc
     1801 catagattcc gttagacgaa tttcaagcta ccttgttcga ggcgacgata atagcaggag
     1861 tatctatatt tatccttttc ttttgtgtgg caggcaccct ggccctcgag ttctgcgaga
     1921 aataggagag agttttgttc gcagttgacc cggatctcct tcaatcgcaa acgattgcct
     1981 gaagtgtcac cctttaccca gatttctttt cgcgacgtgc tgaagaaagt gacgaatcca
     2041 gtttcaatgc tttgcttcag tgcggcatcg tttacgtatc caaggattaa gacgtctttg
     2101 gtcttcgcgt cctgaacaat gactggtaag actgcacctg catcggctgc cttcctggtt
     2161 ttttcaaaat ccagcgagag ggttttgcct tcttctactt cgatcatgga gcaacactgt
     2221 ttgtgtgacg cccggctgca tctcgaaatt ctggtcgtaa tcctgccggg cagacaaaaa
     2281 ataatcccgt gcaggaactt cttctcaagc gccatagtgt ctttcgcggc gatgtgacgc
     2341 tccccggatc aaaatcgatc gccaatcgcg ctcttttgct ctcaagcctg gcacagggaa
     2401 agacgatcct gcgaggtctg ccagatgcag atgacgtgac tgtgtttcgc cgcaaccttc
     2461 cgaacctcgg aattcaggcg accgagatgg gagactctct ggaaattgtg ggtgcgggag
     2521 gttcttttcc cgtgcaaaaa gccgacatct ttgttgagaa cgcaggtacg gccatgcgac
     2581 ctcttgcggc cgctctctg
//
LOCUS       DAOQJL010000550         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28473_length_2595_cov_3.693307, whole genome shotgun sequence.
ACCESSION   DAOQJL010000550 DAOQJL010000000
VERSION     DAOQJL010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28473_length_2595_cov_3.693307"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLG78_17795"
     CDS             <1..737
                     /locus_tag="PLG78_17795"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNE25031.1"
                     /translation="IKICAVVAPGSTDPVKRLSVNLDHVATLREQRHTPYPDLTRAAD
                     LARAGGADGITLHLRGDRRHIQESDLAALSGGLLPVTLEIACTPENMEILRKYKPFAV
                     TFVPERPEELTTEGGMNLSVASAMLARLIPEIRSLGIRVCLFLEPDEKALRLAKDLGA
                     TMVEIHTGGYAEGRVSVEEILQAADAGDSMGLEINAGHGLTVENARRLGQGKIHEFSI
                     GHSIICRSVMVGLEEAVREMKAAIQG"
     gene            769..2505
                     /locus_tag="PLG78_17800"
     CDS             769..2505
                     /locus_tag="PLG78_17800"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNE25032.1"
                     /translation="MEFLNIIIDRVDSTNVFNVLRGRMPSRESHLHSMMDNDLIQEYL
                     LEIERLSKIGTSLTGQERDGIFDLTGNLRRMGESFFLQFFPSAVQEMLRSAENGFLFL
                     HTDEQLRQIPWELLHDGVCFLADKFYIGKNISGHWRDRQREERENLRVLIACDPTEDL
                     EWARREGEGLFEALNSEVDPERLDIRFMSGRDITKLSLLDAMKDRDVVHYAGHVDPAR
                     TPGESGWPLSGGKILRTREIEKAGFSPDLVFANGCSVQRSLGESDVASAFLRAGIANF
                     IGTNWEIRDSQNTLDFAVHFYRSIFEEKSLGESLFEARQFARRTYPPADLTWANYVLH
                     GNPMARIYRSNRRFQVDMPALVERWIVEEFPAPIARPYSEFIAYKDANHAPGMLSALV
                     HAFEEFVYLTGAIVFGSGQTVGFRAALPDFGVNAWLSWIHESLAQMSALRMEPALPGL
                     AGVLSLSRENLEKLALWANLYREGKLVGDEAQYVVTFQYIFESILSEAGYLRRMLFLY
                     IDPEGGSVLFQGTSPQPYRILPQDMEDAAVWVREHRGQIVFINSTRKIRFSLHAFYTL
                     NAAGIPSRRMTI"
BASE COUNT          631 a          593 c          718 g          653 t
ORIGIN      
        1 aaatcaagat ttgcgcggtg gttgcacctg gatctactga ccctgtgaag cgtctgtctg
       61 taaacctgga ccatgtggcc actctgcgag agcagcgtca tactccgtat cctgacctga
      121 cccgcgcggc ggatctggcg cgagcgggcg gtgcggacgg aatcactctt caccttcgcg
      181 gagacagaag gcacattcag gaatcggatc tggcggccct gtccggtgga ctccttccgg
      241 tcactcttga aatcgcatgc acgccagaga acatggagat actacgtaag tacaaacctt
      301 ttgctgtgac gtttgttccg gaaagaccgg aagagcttac aacggaaggc ggaatgaact
      361 tgagcgttgc ctcggccatg ctcgctcgtc tgattcctga gattcgttct ctcggaattc
      421 gcgtatgcct gtttttagag ccggatgaaa aggctcttag actggcaaag gatctgggcg
      481 ccactatggt ggagatccac acaggtggtt acgcagaagg gcgagtgtct gtagaggaaa
      541 ttcttcaggc agcggacgcg ggcgattcga tgggactcga gatcaatgca gggcatggtc
      601 tgactgtaga gaatgcgcgt cgtctgggtc agggaaagat ccatgagttt tcgatcgggc
      661 attccatcat ttgtagatct gtgatggttg gccttgagga agctgtgaga gaaatgaagg
      721 ctgccattca gggttgaatc gaactgcaaa gtaacaccgg caataaaaat ggaattcctg
      781 aatatcatca tcgaccgagt ggattcgaca aacgtcttca acgtgctacg cggacgcatg
      841 ccttctcgag agagccacct tcattcgatg atggacaatg atttgattca ggaatatctt
      901 cttgaaattg aacggctgag caaaatcggc acaagcctga ccggtcagga acgagacgga
      961 atttttgatc taacgggaaa ccttcgcaga atgggagagt ctttcttcct tcagttcttt
     1021 ccctctgcag tgcaggaaat gcttcgatct gcggagaacg gttttctttt tttgcacaca
     1081 gatgagcaac tcaggcaaat tccctgggag ctactgcacg atggagtctg ttttcttgcg
     1141 gataagtttt acattggaaa aaatatttca ggacactgga gagatcggca gagagaggag
     1201 agagaaaatc ttcgcgtgct gattgcatgc gatccaacgg aagatctaga atgggccaga
     1261 agagaaggag aaggtttgtt tgaagctctc aacagcgagg tggatccgga acgtctggat
     1321 attcgcttca tgtctggtcg ggatatcaca aagctctctc ttctagatgc gatgaaggat
     1381 cgcgacgtgg ttcattacgc aggccatgtg gatccggcca gaactccagg agaaagtgga
     1441 tggcctcttt ccggaggaaa gatccttcgc acgcgcgaaa tagaaaaggc cgggttttca
     1501 ccggacctgg tgtttgcaaa tggatgttcc gttcaaaggt ctcttggtga atcggacgtg
     1561 gcttctgcat ttctgcgtgc tggaattgca aacttcattg gcaccaactg ggaaattcga
     1621 gattctcaaa acactctgga tttcgcggtg catttctatc gaagcatctt tgaagaaaag
     1681 tctctgggag aatctctttt tgaagccagg caatttgcgc gtagaacgta tccacctgca
     1741 gatcttacat gggcaaacta cgttctccat ggcaacccga tggcgcgtat atatagaagc
     1801 aatcgtcggt ttcaggtcga tatgccagcc ctcgtggaga gatggattgt ggaggaattt
     1861 ccggcaccca tcgctcgacc ctattctgaa ttcatcgcct acaaagacgc aaaccatgct
     1921 cctggaatgt tgtctgctct ggttcatgcg tttgaggaat tcgtctatct tacaggagcc
     1981 atcgtcttcg gaagcggcca gacagtgggc tttcgcgcgg ctcttcctga tttcggagtg
     2041 aatgcctggc tgagctggat tcatgaatct ctcgcgcaaa tgagtgcgct tcgcatggaa
     2101 ccggcacttc ctggtctggc aggtgtgctc agcctttcgc gcgaaaacct ggagaagctg
     2161 gcactctggg caaatctgta cagggaagga aagctcgttg gagacgaagc tcagtacgta
     2221 gttacctttc agtatatttt tgagagcatt ctgtcggaag cgggttatct tcgcaggatg
     2281 ctcttcctct acatagatcc ggaaggcgga tcggttttgt ttcagggaac cagtcctcag
     2341 ccgtacagaa ttctacctca agatatggaa gatgcagcgg tgtgggtgag agagcacaga
     2401 ggacagattg tatttatcaa cagcactcgt aagattcgct tttctttgca tgcgttttac
     2461 actctcaacg cagctggaat tccttcgagg cgcatgacga tttgacaaat cgttttgaga
     2521 gaacatggaa agcgcctgcc agagatctac atccgcctgc ggagcttgct gcggaatctt
     2581 caatctggat ctagg
//
LOCUS       DAOQJL010000551         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28677_length_2584_cov_4.966390, whole genome shotgun sequence.
ACCESSION   DAOQJL010000551 DAOQJL010000000
VERSION     DAOQJL010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_2584_cov_4.966390"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="PLG78_17805"
     CDS             <1..1755
                     /locus_tag="PLG78_17805"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNE25033.1"
                     /translation="KAVHDNGGLIAMQILHTGRYGYHDKAVGASELRAPINVVKPHKL
                     SHQEILSTIDDFARCTELARHAGYDGVEIMGSEGYLINQFTAKRTNNRDDDWGGSLEN
                     RMRFPLEIIRAVRKRVGNDFIIVYRLSMLELVEEGGNLDDVIRLAKEVEAAGVNILNT
                     GIGWHEARVPTIAMMVPRAAFTWVTKNVRQHVKIPLCTSNRINTPEIAEAVLANGDAD
                     MVSMARPLLADSHFVNKAAADKADEINTCIACNQACLDHIFVGTTASCLVNPRACNET
                     ELNYIPATKKKRIAVIGAGPGGMSAAAVAAERGHNVTLYDAAGDIGGQIHIARRIPGK
                     EEFNETLRYFRTILKKFGVTQKLSTRVSAADLIAEKYDEVILATGINPRKLTIPGSDR
                     PNVLSYVDVVLHGKAVGKRVAVIGGGGIGFDVTIYLTESESSADRKTAFLVEWGIDPS
                     IKVSGGLLPEQKHPAAPRQVTMFKRSKGKFGGNLGKTTGWIHRQTVEDRHVEQISGVT
                     YEKIDDQGLHYSLKGQNHVLPVDTVVVCAGQEPNQELLGPLKSAGVSVHVIGGALEAA
                     ELDAKRAIDQGARLAASL"
     gene            1844..>2584
                     /locus_tag="PLG78_17810"
     CDS             1844..>2584
                     /locus_tag="PLG78_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE25034.1"
                     /translation="MNGGELIAQTLQLHGVRAVFTLIGGHISPILVSCKKAGVRVVDV
                     RHEVNTVFAADASARLTGIPGVAVVTAGPGVTNTLTALKNAQMAQSPLVLIGGATATV
                     LKNRGSLQDIDQIAAVKPHVKWHASAKKVRDLPRLIATAFMKSREGVPGPVFVEVPVD
                     LLYEESTVREWYMASVPRKPGLKNYVISRYLSRHVDTLFRGADLSSPASPMPYNVPSH
                     TDSDLRQVVERLQTAAKPLLVIGSQAMLQ"
BASE COUNT          657 a          622 c          711 g          594 t
ORIGIN      
        1 aaagccgtgc acgacaacgg cggactcatc gcgatgcaga ttctgcacac cggcagatac
       61 ggatatcacg ataaagcagt cggagcatct gaactgcgcg ctcctatcaa tgttgttaaa
      121 ccgcacaagc tttcgcatca agaaattttg tccacgatag acgactttgc gagatgcaca
      181 gaactcgctc gacacgcagg ctatgacgga gtagagatca tgggctccga aggctacctg
      241 atcaatcaat tcaccgcgaa gcgcacgaac aatcgcgatg acgactgggg aggatctctt
      301 gaaaacagga tgcgttttcc tctcgagatc attcgcgctg tgcgaaagag ggtaggaaat
      361 gatttcatca tcgtgtatcg cctttccatg ctggagctgg tggaagaagg cggaaatttg
      421 gacgatgtga ttcgccttgc gaaggaagtg gaagcagccg gcgtgaacat tctaaacaca
      481 ggcataggct ggcatgaagc gcgagtgccg accattgcaa tgatggttcc tagagccgca
      541 ttcacatggg tcacgaagaa tgttcgtcag catgtgaaga tacctttgtg tacgtccaat
      601 cggatcaata ctccggagat tgcggaagcc gttcttgcaa acggggatgc ggatatggta
      661 tccatggcca ggccgttgct tgcggattct cattttgtaa acaaagcagc cgcggacaag
      721 gcagacgaaa tcaacacatg cattgcctgc aatcaggcat gcctcgatca catttttgtt
      781 ggaacgaccg cgagctgcct cgtaaatcca agagcttgca atgaaacaga actcaactac
      841 attcctgcga ctaagaagaa gcgcattgca gtgattggtg ccgggcctgg tggtatgtcc
      901 gccgcggcag tggccgcgga acgcggacac aatgtaacgt tatacgacgc tgctggcgac
      961 ataggcggtc agattcacat tgcgcgcaga attccgggca aagaggaatt caatgaaact
     1021 ctcagatact ttcgcacaat cttaaaaaag ttcggagtta cacagaaact ttctactcga
     1081 gtttccgctg cggatttgat cgctgagaaa tatgatgagg tcatcctcgc gactggaatc
     1141 aatccgcgca aactcacgat tccaggatcg gatcgaccaa acgtgctgag ttatgtggat
     1201 gtagtcctgc acggcaaagc agtcggcaag agagtggccg tgattggagg cggcggcatt
     1261 ggttttgatg taaccattta cctcactgaa tccgaatctt ccgcagatcg caagactgca
     1321 tttctcgtag aatggggaat tgacccatcg atcaaagttt ctggagggct actcccggaa
     1381 caaaagcatc ccgcggcgcc acgtcaggtg accatgttta aaagaagcaa aggaaaattt
     1441 ggcgggaacc tgggcaagac gacaggttgg attcacagac agactgtgga agatcgccat
     1501 gtggaacaga tcagcggcgt cacctacgag aagatcgatg atcaaggttt gcattattct
     1561 ctgaaagggc agaaccacgt tttgcctgtg gatacagttg tggtttgtgc gggtcaggaa
     1621 ccaaaccagg agttgcttgg acctttgaag agcgcaggag tttccgtgca cgtcattggc
     1681 ggcgccctcg aagcagcaga gctcgacgcg aagcgtgcga tcgaccaggg cgccaggctt
     1741 gcggcatcac tatgatataa atcatagtga atcgtgttct ttggtttggc cctggtttct
     1801 gaattatagt tgagtaagtt taacgtcatg cgctcatgag ggcatgaatg gtggcgaact
     1861 cattgctcag acattgcaac ttcacggtgt gcgcgccgtc ttcactctta taggcgggca
     1921 tatatctccg attctcgtga gctgcaagaa agctggagtg agagtagtag atgttcgtca
     1981 cgaagtaaat acggtctttg ctgcagacgc ctcagccaga ttgactggaa tcccaggcgt
     2041 tgcagtggtt acggcaggcc ccggcgtcac caatactctc actgccctca agaatgcaca
     2101 gatggctcaa tcgcctctcg ttctgattgg cggagccacc gcgactgtgc ttaagaatcg
     2161 cggttcattg caagatattg accagattgc tgcggtaaag ccgcacgtga agtggcatgc
     2221 ctcagcaaag aaggtccgcg atctgccgcg cttgattgca actgctttca tgaaatctcg
     2281 cgaaggagtt cccggaccag ttttcgtgga agtacccgtg gatttgctat atgaagagtc
     2341 aactgtgcgt gaatggtata tggccagcgt gccccggaag ccagggctca agaattacgt
     2401 gatatcgcga tatctatcca gacacgtgga cactcttttc cggggagcgg atctttcttc
     2461 gccagccagt ccaatgccgt ataacgttcc ctctcatact gattctgatt tgcgtcaggt
     2521 tgtggagcgg ctacagaccg ccgcaaagcc gctccttgtg attggaagtc aggcaatgct
     2581 gcag
//
LOCUS       DAOQJL010000552         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28678_length_2584_cov_4.614472, whole genome shotgun sequence.
ACCESSION   DAOQJL010000552 DAOQJL010000000
VERSION     DAOQJL010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28678_length_2584_cov_4.614472"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLG78_17815"
     CDS             complement(<1..770)
                     /locus_tag="PLG78_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNE25035.1"
                     /translation="MIPLSSSNFSYANAQANGMDVCFADSSQTQLAFERDVYNAAGNS
                     AFWIRIPTLPSGSTTVYMYYNSQSTCPSTGSVWDSNFRAVYHLSSDPSASVPDSANSN
                     ALTGIAMTAANLTSTQTGYGFLLDGTTQYLTAPDSPTLQIASSLSLEVVYKLSAYTLN
                     AHYLVEKGDSDFDNYSLYLKRTGAFGCTSPIGCAAFEFRDAGSSYFDITGARDVTPGT
                     WHTLAANVDATGGKVDFYLDGSFSESLPASLPGNMWPQV"
     gene            1161..1847
                     /locus_tag="PLG78_17820"
     CDS             1161..1847
                     /locus_tag="PLG78_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNE25036.1"
                     /translation="MDISTLSSDEAFSRFLKFSSIVACAVIAWTRPGPDRRDTVLLRI
                     AFLLTVLADFLLGVLHLFIPGVAVFALVHIVYVMRHLRGISSLVAEGILAGFCLVFAI
                     VIMRLSSPAMARAGLLHESIAYAVPLTFSLYAGIGVMIRDHFRMVHRIRIAAGIVLFF
                     AVDIAVAHFQALPDGLMRTILGMAIWPLYLPSQFLLACSGSGAKQGVPVCVSVPRPGP
                     SNAFLRLRLD"
     gene            1916..>2584
                     /locus_tag="PLG78_17825"
     CDS             1916..>2584
                     /locus_tag="PLG78_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE25037.1"
                     /translation="MSEVKKIKVKINQVEYEVPANWSIIQACHNNGVEVPHYCYHQDL
                     SVAGNCRMCLVKASNAPRPVIACATPLAENMEIDTLSEDVVAARKSVLEFLLINHPLD
                     CPECDQAGECRLQDYSYEYGRDHGRFHEEKNIRAKATLGPHVKYWGSRCIVCTRCVRF
                     TDEISGTGELTVAQRGDRSEITVFPGQTLDNPLSLNTVDVCPVGALVSADFLYRSRVW
                     FMHST"
BASE COUNT          574 a          671 c          680 g          659 t
ORIGIN      
        1 acctggggcc acatgtttcc aggaagagat gcaggtagag attcgctgaa agatccatcc
       61 aggtagaaat ccacctttcc tccggtggcg tctacgttcg cagcgagagt atgccaggtt
      121 cctggcgtca catccctggc acccgtaatg tcgaagtatg aggaaccggc gtccctgaac
      181 tcgaatgcgg cgcaaccaat cggcgaagtg catccgaatg ctcccgttct cttcagatag
      241 agagagtagt tgtcaaaatc actatctcct ttttccacca gatagtgcgc gttcagagta
      301 taggcgctca gtttatatac aacttccagg ctgaggctgg atgcaatctg aagcgtgggc
      361 gaatccggcg cggtgagata ctgcgtggtt ccatccagca ggaatccata ccccgtttga
      421 gtggaagtga gattcgcggc cgtcatcgca atgccagtga gcgcattgga gttcgcgcta
      481 tccggaacag aagcggaggg gtccgaactc agatgataga ctgcgcgaaa attggaatcc
      541 catacactgc cggtagacgg gcaggtgctc tggctgttgt agtacatgta gaccgtagtt
      601 gagccagaag gcagagttgg aatacgaatc cagaaggcag aattaccagc cgcgttgtat
      661 acgtcgcgtt cgaatgccag ctgcgtttga gacgagtccg cgaaacaaac atccatacca
      721 ttagcctgcg cattcgcata actaaagtta gacgatgata gtggaatcaa aaccggaaca
      781 tcggaaagag tggttccatg gttggctata gtgacggatc tgcgcagtcc ccaggatcga
      841 ttccaccacg gggcaatggg acgaaatata ctcagcccca gccacgccga agtggcgtca
      901 tagtgcgcac aattgccgag gagtgtcagt cccagccagg cgcagagcag aattctctta
      961 cccgaacgtg tgatacccca ttccaggcgt ttgcatttgt tttacaagga ttttaactcc
     1021 aatccgtgcg attgcggacc cgcgcagggc gaagcgcaaa atttgtcaca ttgagcatat
     1081 aatatgctcg tatgaccaga gaccaatcgt tcctctttct ttccagcgtt gcctgtattt
     1141 gcttttgcgg tttcgcggcc gtggatatat ccaccctctc ttcagatgaa gctttttcgc
     1201 gctttctgaa gttttcttct attgtggcat gcgctgttat agcgtggacc aggccaggcc
     1261 cggacagacg cgataccgtt cttcttcgca tcgcattcct cctgacagta ctcgccgatt
     1321 tcttgctggg tgttcttcac ctgttcattc ccggagtggc tgtttttgct ctggttcaca
     1381 ttgtatacgt tatgcgacat ttgagaggta tctccagtct ggtcgctgaa gggattctgg
     1441 ctggattttg tctggtgttc gcgattgtga tcatgagatt gtcttctccg gcgatggccc
     1501 gcgcggggtt gttgcatgaa tccatagcct acgcggttcc tctcacattt tcgctctacg
     1561 ccgggatcgg agtgatgatc cgggatcact ttcgcatggt gcatcgtatc cgaattgccg
     1621 cgggcattgt actgtttttt gccgtcgata ttgctgtggc gcattttcag gcccttccgg
     1681 acggcctcat gcgaacgatt ctggggatgg ccatatggcc tctctacctt ccatcccaat
     1741 ttctcctggc ctgtagcgga tcgggcgcga agcaaggcgt cccggtttgc gtttctgtgc
     1801 ctcgaccggg tccttcgaac gcctttttgc gccttcgtct ggattgaaaa aaagcccgga
     1861 agagatcagg aaaatcttgc cgcatgaccg gtcggggcat ccttagcttt gagccatgtc
     1921 ggaagtcaaa aagatcaagg ttaagatcaa ccaggtcgaa tacgaggttc ctgcaaactg
     1981 gtcgatcatt caggcctgcc acaacaacgg agtggaggtt cctcactatt gctatcacca
     2041 ggacctatct gttgcaggca actgccgcat gtgtcttgtc aaagcatcca acgctccgcg
     2101 tcctgtcata gcctgcgcca cacctctcgc ggagaatatg gaaatcgaca ctctatcaga
     2161 agatgtcgtg gctgcgcgca aatccgttct cgaattttta ctgatcaatc atcctcttga
     2221 ttgtcctgaa tgtgatcaag caggtgaatg tcgtcttcaa gattatagct atgagtacgg
     2281 gcgcgaccat ggtcgctttc acgaagaaaa aaacattcgt gcgaaagcca ctctgggccc
     2341 tcacgtgaag tactggggct cacgctgcat cgtttgtaca cgctgtgttc gctttacaga
     2401 tgaaatcagc ggaaccggag aactcaccgt agcacagagg ggagatagaa gcgaaatcac
     2461 agtattccca ggacagacgc tcgacaatcc actctctctc aatactgtgg acgtctgccc
     2521 ggtgggcgcg cttgtttctg cggacttttt gtatcgctcg cgcgtctggt tcatgcattc
     2581 tact
//
LOCUS       DAOQJL010000553         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28855_length_2575_cov_4.617460, whole genome shotgun sequence.
ACCESSION   DAOQJL010000553 DAOQJL010000000
VERSION     DAOQJL010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28855_length_2575_cov_4.617460"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="rfbG"
                     /locus_tag="PLG78_17830"
     CDS             complement(<1..755)
                     /gene="rfbG"
                     /locus_tag="PLG78_17830"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNE25038.1"
                     /translation="MIVDPGFWAGKQVFLTGHTGFKGSWLSLWLSEMKAKVTGYALSP
                     PTDPSLYERAKISQDLNSHIADINDLARLTSAVKDAKPEIIIHMAAQPLVRESYAQPV
                     ETYQTNVMGTMHLLEAARACGGVRSIVIVTTDKCYENKEWPWGYRENDHLGGYDPYSS
                     SKACAEILTAAYRSSYFHPEKYAQHRTALASGRAGNVIGGGDWAKDRLIPDCIAAYLR
                     KEEVIIRNPRATRPWQHVLEPLSGYLCLAQHLYS"
     gene            complement(752..1528)
                     /gene="rfbF"
                     /locus_tag="PLG78_17835"
     CDS             complement(752..1528)
                     /gene="rfbF"
                     /locus_tag="PLG78_17835"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNE25039.1"
                     /translation="MKVVILAGGLGTRISEESHLRPKPMIEIGEKPILWHIMKIYSHY
                     GFDDFVVCLGYKGYVIKEYFSNYFLHESDVTFDFKNQQSMMVHHHKAEPWRVTLVETG
                     METQTGGRVKRVAPYIDKEPFLLTYGDGVGNVDIKALVEFHKSHGRLATVTATQPLGR
                     YGALDFEGEHRVTGFREKPVGDGAWINAGFFVMQPEVLNYIENDSTILERAPLEGLAA
                     KGELHAFKHRGFWQPMDTLRDKRQLEEMWASGKAPWKVWT"
     gene            1592..>2575
                     /locus_tag="PLG78_17840"
     CDS             1592..>2575
                     /locus_tag="PLG78_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25040.1"
                     /translation="MCAVQSLVSMNRSASQAIDHQLFFAGILLACMVLTSCSRSAELK
                     SYVSIPTSIPALEEFGKKNGALVQVERRQKSSGGANCGHSPVCLIVIPFVIAGALFPE
                     KYDVAIATRGQIEIYSGIFSTDGKFVQARTTEPDGIHEFRRLDLPKLHRSIIVEIAKG
                     KEEASIERVPLLPQTHLDEDYRKSIFKSGSQDDRSDLLAEAVDWMGSEADPLVKSVLD
                     DQAFGDLALARFMSYVCEDTEKISSFVRATSLDAALKSSDVTPALNALSCFPSEDPKA
                     APFLRRLAHNVCENAGRSFSYPSEAVRWGGHSSLSVSSLKKRIAGDIDGELS"
BASE COUNT          567 a          638 c          684 g          686 t
ORIGIN      
        1 gaatagaggt gttgagccag acaaaggtag cccgagagtg gttcgagcac atgctgccat
       61 ggtcgcgttg cgcgcgggtt tctaatgatc acttcttctt tgcgaagata agcagcgatg
      121 cagtccggga tcaatctgtc ttttgcccag tcccctccgc caatcacgtt gcccgctctt
      181 ccactggcca gcgcagtgcg atgctgcgca tatttttcgg gatggaagta tgaggaacga
      241 taggcggcag tgagaatttc cgcacaagct ttgcttgagg agtatggatc gtatcctccg
      301 aggtggtcat tctctctgta gccccacggc cattctttgt tttcgtagca cttatctgtt
      361 gtaacaatta cgatggagcg gacacctcca caggctcgcg cagcctcgag caaatgcatg
      421 gttcccatca cattcgtttg atatgtttct acaggttgcg cataagattc gcgcacaagt
      481 ggctgggctg ccatatgaat gatgatctcc ggtttagcat ctttgacggc agacgttagg
      541 cgcgctagat cattgatgtc tgcgatgtgt gagttcagat cctggctgat cttcgctcgc
      601 tcgtagagac tcgggtctgt tggaggcgag agtgcatacc cggtgacctt tgctttcatt
      661 tcagaaagcc acaaagacag ccagctccct ttgaagccgg tgtgaccggt tagaaatact
      721 tgtttgcctg cccagaatcc aggatcaacg atcacgtcca aaccttccac ggagccttgc
      781 cggacgccca catctcttcc agttgtcgtt tgtcacggag ggtatccatg ggctgccaga
      841 atccacgatg tttgaacgcg tgaagttctc cttttgccgc aaggccttcc agtggtgcgc
      901 gttctaaaat cgttgaatcg ttttcgatgt agttcagtac ttctggctgc atcacaaaaa
      961 agccagcatt gatccacgcc ccgtcaccga ccggtttttc gcgaaagccc gtcacgcgat
     1021 gctccccttc aaagtcaagc gcgccgtatc ttcccagtgg ctgcgttgct gtcacagttg
     1081 ccagtctgcc gtgtgatttg tgaaattcaa cgagtgcctt gatgtctacg tttccaactc
     1141 catcgccgta agtgaggagg aatggttctt tgtctatgta cggtgcgacg cgtttcactc
     1201 gtccaccggt ttgtgtttcc attccggttt ctacgagtgt gactctccat ggttctgctt
     1261 tgtgatggtg caccatcatg ctttgctgat tcttgaaatc aaaagtaaca tcagattcgt
     1321 gcagaaagta attggagaag tactccttga tgacatagcc cttgtatcca aggcagacaa
     1381 cgaagtcgtc aaagccatag tgggagtaga ttttcatgat gtgccagaga atcggttttt
     1441 caccgatttc gatcatgggt tttgggcgga ggtgtgactc ttcgctgatg cgagtgccga
     1501 gtccacctgc aagaatgacg accttcacgc caagcattcc gtaaggtagc tcgtcgcagg
     1561 ccagcttttt tttgattgca gggggcgttt gatgtgtgct gttcagagcc tggtttctat
     1621 gaatcgctcg gccagtcagg caatcgacca tcagctcttc ttcgcgggga tactcctggc
     1681 atgcatggtg cttacgtctt gttcgcgttc ggctgaattg aagtcttacg tatccattcc
     1741 aacaagcatt cctgcgctcg aggagtttgg aaaaaagaat ggtgcgctgg ttcaggtgga
     1801 aagaaggcag aagtcatccg gcggtgcaaa ctgcggacac agcccggtct gtctgatcgt
     1861 aattccattt gtgattgctg gagctctttt cccggagaaa tacgatgtgg caattgccac
     1921 cagaggacag attgaaatct attccgggat cttttcgacg gacgggaagt ttgttcaggc
     1981 gcgcacaaca gaaccagacg gaattcacga atttcgcagg ctggatcttc cgaaattgca
     2041 tcggagtatc attgtagaaa tcgcaaaggg caaagaagag gcatccatcg agcgcgtacc
     2101 gctcctgcca cagacacacc tggacgaaga ctatcgaaaa tccatattca agagcggttc
     2161 acaggacgat cgttcagacc ttcttgcaga agcggtggac tggatggggt ccgaagcgga
     2221 tccactcgtg aaatcagtgt tagacgatca ggctttcggg gatcttgctc ttgcgcgatt
     2281 catgtcctat gtctgcgagg acacagaaaa gatttcgagc tttgtgcgcg caacctctct
     2341 cgatgcggct cttaagtctt cagatgtcac gcctgctttg aatgcacttt cttgttttcc
     2401 ctcggaggat ccaaaggcag ctccattctt acgcagattg gcgcataacg tctgtgaaaa
     2461 cgcggggaga tcattttctt acccgtcaga ggcagtgcga tggggcgggc attcttcgct
     2521 ttcggtgtcg agtctgaaaa agcgaatcgc cggtgacata gacggtgagc tatca
//
LOCUS       DAOQJL010000554         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28966_length_2569_cov_4.778043, whole genome shotgun sequence.
ACCESSION   DAOQJL010000554 DAOQJL010000000
VERSION     DAOQJL010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28966_length_2569_cov_4.778043"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLG78_17845"
     CDS             <1..392
                     /locus_tag="PLG78_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25041.1"
                     /translation="MADGNLTTSWQTSTPHDSGVLVEFNNPVNLVKLRIANGFQFMNH
                     PRFGNLLEKNSRVRGMALAMEVKDSDGSISTVTKEIELRDRTEAGYDDVALEQNNVLR
                     IAFKPLSIYPGSKWADLAISEVIFFKK"
     gene            408..1058
                     /locus_tag="PLG78_17850"
     CDS             408..1058
                     /locus_tag="PLG78_17850"
                     /inference="COORDINATES: protein motif:HMM:NF028114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HNE25042.1"
                     /translation="MKRYATSPDAPMTTYILYESKVNCSFTFLPEDQSGPTEPDDVEV
                     WRVEAPSFEIACLKRNEYLGWEPYRPQICSEHDLHALLPRSKHDLDNVQLLMNAGLPE
                     IKPVLPQLFEWIQDINWPVARLLLPFLRSLGSKCREEIQSVLQSDDAMWKYWIITELI
                     AKMDQIEARAFMPDLENLLATATLEDKNNEVEAVAAEVLRALRSGKDSSSIDAAGR"
     gene            1064..1771
                     /locus_tag="PLG78_17855"
     CDS             1064..1771
                     /locus_tag="PLG78_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNE25043.1"
                     /translation="MPPKFLFLGPSHLVMLVLTVFLTVAFIVLARRWPHSRLPSILGA
                     LPGLALICSYISYNVQRFRDGYWEPRYDLPMELCTWVAIAVIVTCFTRNLFTFELSYF
                     WVMAGSIHGTLTPNLQEDFPHFYFFIYFIGHVCLVIASFYFIFAWKLRPRPGAIWRVF
                     LMSELYFATAFTLDYALDTNYGYLMRKPTNPSFLDYLGPWPVYLIVLQILALGIFYLL
                     NLPFRLATRGKRVEIRD"
     gene            1816..2307
                     /locus_tag="PLG78_17860"
     CDS             1816..2307
                     /locus_tag="PLG78_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25044.1"
                     /translation="MDPYTAPESIPSPSSEKKNTAAQQALRNPATILLILAILSLFAL
                     PVTALGQLMKKTAENQTFLDSALEKTEKQDAKKSEAFRKGEQFGEFVGKVLAFTLWAI
                     SLPIQLIGFWRMRQGRSYGLSLAAAILACIPCFSSCCGIMLPFAIWSLAVMSRPEVKA
                     SFT"
     gene            2367..>2569
                     /locus_tag="PLG78_17865"
     CDS             2367..>2569
                     /locus_tag="PLG78_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25045.1"
                     /translation="MARVILLLLLTAGCMATPSRPSQPAHQSTAAVSPLRFPPASQSL
                     SENAESVGPEFHAWYSAGHMTQVR"
BASE COUNT          612 a          705 c          616 g          636 t
ORIGIN      
        1 acatggcgga tggaaatctg accaccagct ggcaaacttc tacgcctcac gactccggag
       61 tgctcgtgga attcaataac ccggtgaacc tggtgaaact gagaatcgca aatggtttcc
      121 agttcatgaa tcaccccaga ttcggcaacc tcctggaaaa gaattcgcgt gttcgcggca
      181 tggcgctcgc catggaggta aaagactccg atgggtccat ctccacagtg accaaagaaa
      241 tcgaactgag agatcgcaca gaagcaggat acgatgatgt cgctctggag caaaacaatg
      301 tactgaggat tgcttttaag cctctctcca tatatccagg gtccaaatgg gcggatctag
      361 cgatcagcga agtgatcttc tttaagaaat agatgacttg tcgggagatg aaacgttatg
      421 cgacatctcc cgatgcccca atgaccacat atattctata cgaatcgaaa gtaaattgct
      481 cattcacgtt cctaccagag gaccagtctg gacctacgga gccggatgat gttgaggtat
      541 ggagagtaga agctccctcc tttgagattg cctgtctcaa gagaaatgaa tatctaggct
      601 gggaaccata cagaccacaa atctgttccg aacacgacct tcacgctttg ctgcctcggt
      661 ccaaacacga tctggacaat gtgcagttac ttatgaatgc aggattgccg gaaataaagc
      721 cagtgcttcc acagctgttc gagtggatcc aggacatcaa ctggccagtc gcgcgtttgc
      781 tattgccgtt cctgcggtct ctcggctcaa aatgcaggga agagatccag agcgttttac
      841 agtcggatga tgccatgtgg aagtactgga tcatcaccga gctgatcgca aaaatggatc
      901 agatcgaagc aagagccttc atgcctgacc tcgagaatct cctggcaact gcaaccctgg
      961 aagataagaa caacgaagta gaggcggttg ctgcggaagt tttgcgggct cttcgttcag
     1021 gaaaagattc gtctagcatt gatgccgctg gtcgatagca actatgcctc ctaagtttct
     1081 ctttcttggt ccttcgcatc ttgtgatgct cgtgctcact gtattcctca ctgtcgcctt
     1141 catagtcctt gcacgtcgat ggccgcattc ccgacttccc tctatactgg gagcgctccc
     1201 ggggctcgca ctcatctgct cttacatttc gtataacgtg caacgcttcc gcgatggcta
     1261 ctgggaacca cgttacgacc tgccgatgga gctttgcacg tgggtggcga tcgcagtcat
     1321 cgtcacttgc ttcacgcgaa atcttttcac ttttgaactg tcctattttt gggtgatggc
     1381 cgggtccatc cacggcacgc tcacgcccaa tctccaggag gactttcctc acttctattt
     1441 cttcatctat ttcattgggc acgtctgtct tgtcattgca tctttctatt tcatctttgc
     1501 gtggaaactc aggcccagac ccggggcgat ctggcgtgtg tttctcatgt cggaattata
     1561 ctttgctacg gcattcactc tggactacgc actggacacg aactatggat atctgatgcg
     1621 caagccaaca aatccatcct tcctggacta tctcggtccc tggccagtct atctcattgt
     1681 gctccagata cttgcgctcg gcatttttta cttgttgaat ctcccctttc gccttgcaac
     1741 gagagggaaa cgcgtggaga ttcgagatta aatcaccgcc tactagctaa caccaaaaac
     1801 acgaaggaca aatcaatgga tccgtataca gcacccgaaa gcatcccgtc tccctcttca
     1861 gaaaaaaaga acacggctgc acaacaggcc ctgagaaatc ctgcgacgat tctccttatc
     1921 ctggccattc tatccctctt cgcccttccg gtcaccgccc tcggccagct catgaagaag
     1981 actgcggaaa accagacgtt cctggactcg gccctcgaaa aaacagagaa gcaggacgca
     2041 aaaaagtctg aggcatttcg aaagggggaa cagttcggtg aatttgtcgg gaaagtcctc
     2101 gctttcaccc tctgggccat cagcctccca attcagctga ttggcttctg gcggatgaga
     2161 cagggacgta gctacgggct ttcgctcgcc gcggctatac tcgcgtgcat tccatgcttt
     2221 tccagttgtt gcggcatcat gctgccattc gccatctgga gcctggcagt catgtcacgt
     2281 ccagaggtga aggcatcttt cacctagtcg gaggtccgag tgggatggat aaacgttatg
     2341 cgacatggac aatgaggggg actttcatgg cacgagtcat cttactcctg ctcttaactg
     2401 cgggttgcat ggccactccg agtcgaccgt cgcaaccggc acaccaatcc acagccgccg
     2461 tcagtcccct tcgatttcct cccgccagtc agtccctatc agaaaatgct gaatctgtcg
     2521 gaccagaatt tcatgcatgg tattctgccg gccacatgac ccaggtgcg
//
LOCUS       DAOQJL010000555         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_28986_length_2568_cov_3.760844, whole genome shotgun sequence.
ACCESSION   DAOQJL010000555 DAOQJL010000000
VERSION     DAOQJL010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28986_length_2568_cov_3.760844"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PLG78_17870"
     CDS             complement(<1..1637)
                     /locus_tag="PLG78_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNE25046.1"
                     /translation="MKQGCKSRTIARILGLAALFAVSHALAPQTPPPTSTRDSCTPTK
                     NTFCVSYRDIEIADFLKLMAQIAKKNVLVDETIKGKITILSHSPIPVNRALDFMKQVL
                     ELRGLTVLEEPNLVKVVPMDVASSASIPDPNRPTDQDTTLTSQILRVPSSVNINDLAN
                     VLKSLAGKNTVVAPYKAANTIIITGYAPNVRRVLSLANELIPRLDVPGGTNVETDTVH
                     IYRVKNMPAESLAQTLTRLDNPQAAATPAQPANPAQPGQPPVPVPVPVSVPETPGKPA
                     GKIRAVSHKESNSIIVTASDAEWREIEQIIRQLDMFRQQILLEVLIAEVSSTDLNDFG
                     IDWRYQGTNSGHSQFNTGLAAEGKLVDPDTGKITGNNTLSGFSLGFLKKGGELLGIFN
                     ANMNNQNFNVLSAPQVLTLDNQEAEINVGQDVPVRTQERTSGGGSSEATVNSFEYRPS
                     GIKLKFTPHVNAEGMISIDLFSEVTNIEGASRSAGNPTFNKRNVKTYITVGNKQTIVI
                     GGLVGSEKQEAVSKVPLLGDIPVLGFLFRRTTISDKRT"
     gene            complement(1651..2499)
                     /locus_tag="PLG78_17875"
     CDS             complement(1651..2499)
                     /locus_tag="PLG78_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25047.1"
                     /translation="MLEIVHRLQKNVFLVTLGAAVFLGFSLAYLLTNIIPFFISDSGS
                     VQIASSSDRRPVEVDVVRPLQDFEGVITGNFIRDSGDAGTKGPGTETGEGEITLLGVL
                     SGPPQFARAMVQIVGKPEIETYRLGEAVAGFKIIQILGNSVVVERGSNNLRIYVGEKS
                     GEAAAAPQAGNAPSAQGATKINITRDRLLASTKNPEMIYQNKFAPVTRDGKVLGLKLL
                     FVPQGNFLYELGARSGDIIRRFNGEPLENQDRMIQMWQSLQSANRITIEVERGGKVIP
                     FEILIQ"
BASE COUNT          550 a          642 c          681 g          695 t
ORIGIN      
        1 gtgcgtttgt ctgaaatggt ggtgcggcga aacaaaaatc caagtacagg aatgtctcca
       61 agaagtggaa ccttggaaac tgcttcttgt ttttctgatc ctacgagacc gccgattacg
      121 atcgtttgct tgtttccgac tgtgatgtat gttttcacat tacgcttgtt aaacgttgga
      181 ttgcctgcag atctgcttgc gccttcaatg ttggtcactt cgctgaacaa atcaatcgag
      241 atcattcctt ctgcattcac atggggagtg aacttcaact tgatcccgga cggtctgtat
      301 tcgaatgaat tcacggttgc ttctgagctg ccgccgccag atgttctttc ctgtgtgcga
      361 accgggacgt cctggcccac gttgatttcc gcttcctggt tatcgagagt gagaacctgt
      421 ggagcggata gaacgttgaa gttctggttg ttcatatttg cgttgaatat gccaaggagt
      481 tctccgcctt tcttcaagaa tccaaggcta aatccggata gagtgttgtt tcctgtgatc
      541 tttccggtgt ctggatccac cagctttcct tccgcagcca gaccagtgtt gaactgggag
      601 tgaccggaat tcgtgccctg gtatctccaa tcgattccaa agtcgtttag atctgtagag
      661 cttacctctg cgatgagaac ttctagaaga atttgctgac ggaacatgtc gagctggcga
      721 atgatctgtt caatctctct ccactccgcg tcagatgcag tgacaatgat cgaattcgat
      781 tctttgtgag acactgcgcg aatcttcccg gcaggtttgc ccggagtttc aggaacggaa
      841 acgggcaccg gaactggaac aggaggctgt cctggttgcg ctggattggc tggctgagcc
      901 ggtgtggcag cagcctgagg attgtcgagc ctggtgagag tctgtgcgag actttctgca
      961 ggcatattct ttacgcgata gatgtgaacg gtatctgttt ctacgtttgt tccacctgga
     1021 acgtccagtc gtggaatcag ttcgttggcc agggacagga cacgtctaac gttaggcgcg
     1081 tagcctgtga tgatgatcgt gtttgctgcc ttgtagggag ctacaaccgt atttttcccg
     1141 gcgaggctct tcagtacgtt tgccagatcg tttatgttca cagaagaagg tacgcgaagg
     1201 atctgcgaag tcagagtggt gtcttgatcc gtgggccggt ttggatccgg aatggacgca
     1261 gaacttgcca catccatggg aaccactttt acgaggtttg gttcttcaag gacagtcaac
     1321 ccgcgcaatt ccagaacctg cttcatgaag tccagcgcgc gattcaccgg aatcgggctg
     1381 tgcgaaagga tcgtaatctt gcccttgatg gtttcgtcta ccagtacgtt ctttttggcg
     1441 atctgtgcca tgagctttaa gaagtccgcg atttcgatat cgcgatagct cacgcagaaa
     1501 gtattcttag taggagtgca ggaatctcgc gtagatgtgg gaggcggagt ctggggcgca
     1561 agcgcgtgcg aaaccgcaaa aagggctgcg agacccagta tgcgtgcaat ggtgcggctc
     1621 ttacatccct gtttcattgt aacttccgtg tcactggatc aggatttcga agggaatcac
     1681 tttccctccc cgctcaacct cgattgtgat cctgtttgca ctctgcaaac tctgccacat
     1741 ctgaatcatg cggtcctggt tctccagggg ctctccattg aatcttcgga taatgtcacc
     1801 gctgcgtgca ccgagttcgt aaagaaagtt gccctgcggg acaaaaagta acttgagccc
     1861 gagcaccttt ccgtcccggg tcaccggcgc gaatttattc tgatagatca tttccgggtt
     1921 cttggtggat gcaagaagac gatctcgcgt gatgttgatc tttgtggccc cctgcgcgct
     1981 cggtgcattt ccagcttgag gggccgctgc tgcttctccc gacttttcgc ctacgtaaat
     2041 ccgcaggttg ttgcttccgc gctctacgac cactgaattg ccaaggattt gaatgatttt
     2101 gaatccagcc actgcctcac ccaggcgata cgtttcgatt tctggctttc ccacaatctg
     2161 aaccatcgcg cgcgcgaatt gaggtggacc ggaaagaaca cccagtagag tgatttcgcc
     2221 ttcgcctgtt tctgtcccgg gacctttggt gccagcatcg cccgaatctc gaatgaagtt
     2281 tccagtgatg acaccttcga aatcttgcaa aggccgaacg acgtctactt ccacgggacg
     2341 cctgtcagat gaactggcga tttgaacgga gccgctgtct gaaatgaaaa acggaatgat
     2401 gttggtcagc aggtatgcca gtgagaatcc gaggaaaacg gcggctccca gtgtaactag
     2461 aaatacgttc ttctgtaagc gatgtacgat ctccagcatg cctctttcat gttaccgtgc
     2521 ctctgcaacc tgacccggtg gaacgacctc cttccagcca agtgcgac
//
LOCUS       DAOQJL010000556         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_29250_length_2554_cov_4.361345, whole genome shotgun sequence.
ACCESSION   DAOQJL010000556 DAOQJL010000000
VERSION     DAOQJL010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29250_length_2554_cov_4.361345"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLG78_17880"
     CDS             <1..579
                     /locus_tag="PLG78_17880"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE25048.1"
                     /translation="LQDKSMSDRENIAKSLGWMGESASSTVLISVLKEKDGEGRYGAA
                     WSLGVLRTPDGLGPLLEAADSGDRKLRQVAIEAIGSYGSSDSLPVLSKLLKDSVLSIY
                     AVDAISNIKGEEARKLLTDHAKNQTGGTRKAAIMALGRRKEEASVPFLIQMLREDGTG
                     EIQQSCMNALTEITGERFRTRGNWFDWFEKRQ"
     gene            626..985
                     /locus_tag="PLG78_17885"
     CDS             626..985
                     /locus_tag="PLG78_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE25049.1"
                     /translation="MPSEFSITTAIEEQIPILHIAGEITSESEEELLGAYDRIPGERK
                     NRVILHFSETRYINSSGIAILIGVINRANEVKGRVEFAGLSPHFRKVMDIVGLTDFVK
                     VYDSLQDALQASSANQS"
     gene            1064..1924
                     /locus_tag="PLG78_17890"
     CDS             1064..1924
                     /locus_tag="PLG78_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25050.1"
                     /translation="MRSLCPGAAILALLILAYPSFASPVSRYVDSFYPSKPTGELRLN
                     YYYDRTGKWVKFSDWIPYRKQRIQPRFFEDFYQIYGLPPAYNTGEVKESIYYLAAALG
                     TQFRHPSRALCTIRTEEEYHKYRNLMYMQTNLLIMRMFLRLGSLYDKRHLYFHDLDVA
                     DDLSVSFRIAKAYYEQAKPFWELARKHATVASQYRFDLDLPGIESERYRIMTGETNFD
                     RIIERHIADVDAKIQATDTFLAREGGSPRPVKAAMQKDIESMYDSEFTPDPLGPPVLQ
                     PHWNDKDPEP"
     gene            1939..>2554
                     /locus_tag="PLG78_17895"
     CDS             1939..>2554
                     /locus_tag="PLG78_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25051.1"
                     /translation="MRRAAVIAIFFFRTLGAQTIADFEEEYRLMTEREGFRGNPVAIE
                     RDWAKAESVPDPFPETPYVSQMEREEAQLRRAYSLYESAREIASELRQQLDAKPGRSI
                     RDPLWWKELDESAAESRRQRALRQRFAIDLAERYHRVFESLQELDRTAFRQDKRVISL
                     KRMAYRQYIILQSAVGNYIPAIEVLDEYARLEGAQTEWPLHYYYY"
BASE COUNT          668 a          614 c          664 g          608 t
ORIGIN      
        1 cttcaggata aatccatgag tgatcgcgag aacattgcga agtcacttgg atggatggga
       61 gaatctgcca gttcgactgt tctcatttct gttttgaaag agaaagacgg ggaagggcga
      121 tacggtgctg cgtggtccct cggagtgctt cgcactccag atggactggg acctctcctc
      181 gaagcggctg attcagggga tcgcaaactc agacaagttg cgatcgaagc gatcggttcg
      241 tatggatcgt ctgattctct ccctgttctt tcgaagttgc tcaaagattc tgtgctcagc
      301 atttacgctg tagacgcgat ttcgaatata aaaggagaag aagcgcgcaa gttattgacc
      361 gatcatgcaa aaaatcaaac gggcggcacg cgaaaagcgg ccatcatggc tctgggtaga
      421 agaaaggaag aggcatctgt acctttcttg atacagatgc ttcgggaaga cggaaccggc
      481 gaaattcagc aatcctgcat gaatgcactc accgaaatca cgggggaaag gttccgcact
      541 cgaggcaact ggttcgactg gttcgaaaaa aggcagtaga aaagaattgg cagaagcaag
      601 ggttatttga ttccttaatt aaagcatgcc ttctgagttt tccatcacaa ctgcgattga
      661 ggaacagatt cccatccttc acattgcagg ggaaatcacg tccgaatcag aagaagagct
      721 tctgggcgcc tatgatcgaa tcccgggcga acgaaagaac cgggtcattt tgcacttctc
      781 tgagaccaga tacatcaatt cttccgggat cgccattctc ataggggtta tcaatcgagc
      841 caacgaggtc aaaggcaggg tagagtttgc aggcctgagt ccccattttc gcaaggtgat
      901 ggacattgtc ggattgactg actttgtcaa agtgtatgat tctctgcagg atgcgctgca
      961 ggccagttcc gccaaccaga gctaacggcg cccattcctt ccagacgcgc tcgttcgtga
     1021 gcattgccta acgcttttgt ccagatcggc cgattctccg tttatgcggt ccttatgccc
     1081 cggagcggca attcttgctt tgttgatcct ggcctatcct tcttttgcct ctcccgtgtc
     1141 gcgctacgtg gactcttttt atccctccaa accaacagga gagctgcgcc tgaactacta
     1201 ctatgaccgg acgggcaaat gggtaaaatt ttcagactgg attccttata gaaagcaaag
     1261 gatccagccg cgattctttg aagactttta ccagatctac ggcctgcctc cggcttacaa
     1321 tacgggggaa gtgaaagaga gtatttatta tctggcagcc gccctgggca ctcagtttcg
     1381 tcatccatcg agagccctct gtaccatccg aacggaagaa gaataccaca agtacagaaa
     1441 ccttatgtat atgcagacga atctgctgat catgcgaatg tttcttcgtc tgggttctct
     1501 ctatgacaag aggcatttgt attttcatga cctcgacgtt gcggatgatc taagtgtttc
     1561 ttttcgaatt gcaaaagcat actacgagca ggcgaaacca ttctgggagc tcgcgcgaaa
     1621 gcacgcgact gtggccagcc agtacaggtt tgatctagac cttcctggaa ttgagtcaga
     1681 gcgctatcgc atcatgacag gcgagacgaa ttttgaccga atcattgagc gtcacatcgc
     1741 agacgtggat gccaaaattc aggcaacaga tacattcctg gcccgcgaag gaggatctcc
     1801 gcgcccggtc aaggcagcca tgcagaaaga catcgaatcc atgtatgatt ctgagttcac
     1861 tccggatccg ctcggaccac ctgtgcttca gccgcattgg aatgacaaag atcccgagcc
     1921 atgaaaagaa tctaaatcat gagacgcgcg gcagtcattg cgattttctt ttttcggacg
     1981 ttaggcgctc aaacgattgc tgatttcgaa gaagagtaca ggttgatgac agagcgcgaa
     2041 gggtttcgcg gaaatccagt cgccattgag cgcgactggg ccaaggcgga gagtgtaccc
     2101 gatccatttc cggaaactcc ctacgtttcg cagatggaga gggaggaagc acagcttcgt
     2161 cgggcatata gtctttatga aagcgcgcgt gagattgcct cagaattgag gcagcaacta
     2221 gatgcaaagc ctggccgaag catccgagat ccgctgtggt ggaaagaact ggacgaaagt
     2281 gctgctgaaa gtcgcaggca gagagcactc cggcagagat tcgccattga tctagcggaa
     2341 agatatcaca gagttttcga atccttgcag gagttggatc gcaccgcatt cagacaggac
     2401 aaacgagtca tctctctcaa gagaatggct tatagacaat acatcatact ccagtctgca
     2461 gttggtaact acattcccgc cattgaagtg ctcgatgagt atgcgcgtct ggaaggagca
     2521 caaacagagt ggcctttgca ctattactac tatg
//
LOCUS       DAOQJL010000557         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_29395_length_2547_cov_4.967897, whole genome shotgun sequence.
ACCESSION   DAOQJL010000557 DAOQJL010000000
VERSION     DAOQJL010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29395_length_2547_cov_4.967897"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLG78_17900"
     CDS             <1..471
                     /locus_tag="PLG78_17900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE25052.1"
                     /translation="VEWITQAFLELQHVFESFDCSMLVSCVLGLVEEETGLLTYVSAE
                     HPSVVLFRDAKASFLDTETAHLKLGTPHTITDVHIRSFQMKKGDHIIAGSDGRDDLHL
                     GGANIRIMNENELLFLEIVERAKGDLKEIAAQLEKEGSLTDDLTLLRLAYNAKN"
     gene            complement(434..1351)
                     /locus_tag="PLG78_17905"
     CDS             complement(434..1351)
                     /locus_tag="PLG78_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE25053.1"
                     /translation="MDLLSGILQQARWTNHMLLRKTIRGSWGFRFPCDRSGGFHVITL
                     GSCYLVHDEEEVKLEKGDLVFVMRGVPHDLVSEPGQKVLDISKFSEASMRTRNGPVVQ
                     ITSMRYEFPDGDAHSFFRELPPIVLVRGSEIPPHHPLQTALSLLSAEQGNENGSALIL
                     ERLTDIMLFYALRHWLGSHPARQPGFRSAMLDEKISSVLDSVHRKPAHAWTLDGLARM
                     VGMSRASLASRFRGAMGMTVMDYVARLRLDAGRVLLEDESMTLEDAAQAVGYSSAFAF
                     SKAFKRVFGRSPRSPLPANSSRYKLTAVR"
     gene            1479..2327
                     /locus_tag="PLG78_17910"
     CDS             1479..2327
                     /locus_tag="PLG78_17910"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE25054.1"
                     /translation="MKIFVYGATGMVGQNVVNQLLAAGHEVFAGSRKPESGKASRGLT
                     WVKADAANPQAGVEILDKVDRAFFIAPPGFTDQYAILSPWLNRAKEKKLQKVVLMTAM
                     GVEHAPAEAPFRKVELMLEGSGLNYNIVRPNWFMQNFQTFWIGGIKSDGKIYFPGGDA
                     KASFIDARDISSSVVSLLTEDKFNNQAFALTGPAAVTHDEVAAALSSVSGKSITYVNV
                     TPEDFKKGLLSGGVPNDYADFLVFIAGNLREGHASPVTATVKQITGKEPIAFQQYAND
                     NQAAWK"
BASE COUNT          718 a          648 c          634 g          547 t
ORIGIN      
        1 gtagaatgga tcacccaggc ttttctggaa ttgcagcacg tattcgagag cttcgattgt
       61 tctatgcttg tttcctgcgt gctcggcctg gtagaggaag aaactggact gttgacctac
      121 gtttccgcag agcatccgtc tgtagtactg ttccgtgacg caaaagcttc ctttctggat
      181 acagaaactg cacacctcaa actgggcacc cctcatacaa tcacagacgt tcacattaga
      241 tccttccaga tgaaaaaagg agatcatatc attgctggct cagatggaag agatgatcta
      301 catctgggag gagcgaatat tcgcattatg aacgaaaacg aactcctctt tcttgaaatc
      361 gtggaacgcg caaaaggaga tctcaaagag atcgccgctc agctggaaaa agaaggcagc
      421 ctcacagacg atctcacctt actgcggtta gcttataacg cgaagaatta gccggcaaag
      481 gagaccttgg agatctgcca aagactctct tgaaagcctt ggaaaaagca aaggcagagg
      541 aatacccaac tgcctgggct gcatcctcaa gagtcataga ttcatcctca agcaaaacgc
      601 gtccggcatc cagcctcagt ctcgcaacat aatccatcac agtcattccc atggcgcctc
      661 gaaagcgaga agcaagcgaa gccctggaca ttcccaccat acgcgccaga ccatccagag
      721 tccaggcatg agcgggcttt ctgtgaacag aatccaatac tgatgaaatc ttttcatcca
      781 gcatggcgct gcgaaatcca ggctgacgcg cagggtgtga tccaagccag tgtcgcaggg
      841 catagaatag cataatatca gttagtcgct cgaggataag ggcggacccg ttttcattgc
      901 cctgttctgc agaaagaaga gaaagtgcag tctgaagagg atggtgagga ggaatttctg
      961 atcctcgcac aagaacgata ggcggcaatt ctctgaagaa cgaatgcgca tctccgtccg
     1021 gaaattcgta tctcattgaa gtgatctgca ccacggggcc gttcctggtg cgcatagaag
     1081 cctcagaaaa tttggatatg tccagaacct tctggcctgg ttctgaaact agatcatgag
     1141 gaacaccgcg catgacaaat actagatccc ccttctcaag cttcacttcc tcttcatcgt
     1201 gcaccagata gcaactgccc agagtgatga catgaaagcc accgctgcga tcgcagggaa
     1261 aacgaaagcc ccaggagcct cgaattgtct ttcgcaaaag catgtgattg gtccagcgtg
     1321 cctgctggag aataccggaa agaagatcca tgatcccaaa aatataccgg gatgttcaaa
     1381 cgtccaatga tttatactcc tggatatgct tctttggacg tccggacatt gccagaacgc
     1441 aatctgtgcg ctacacttag atcagaacgg aggaaagtgt gaaaatattt gtatatggcg
     1501 caaccggaat ggttgggcaa aacgtagtga atcaactgtt ggccgcaggc catgaggttt
     1561 ttgctggaag ccgcaagcca gaatcaggaa aggcgtccag agggctcacc tgggtgaagg
     1621 cggacgctgc caatccgcaa gcaggcgttg aaatcctgga caaagtagac agagcattct
     1681 tcatcgcccc gcccggcttt acagaccagt atgcaatcct cagcccctgg ctgaatcgcg
     1741 ccaaagaaaa gaagcttcaa aaagtagtac tgatgactgc gatgggagtg gagcacgctc
     1801 cagcagaagc gccctttcgc aaggtagaac tcatgctgga aggctccggt ttgaactaca
     1861 acattgtgag accgaactgg ttcatgcaaa acttccagac attttggatc ggcgggatca
     1921 aatcagacgg taaaatctac tttccgggcg gagatgcgaa agcaagcttc atagatgcaa
     1981 gagacatctc ttcatccgtt gtttctctgc tcactgaaga taaattcaac aaccaggcgt
     2041 tcgcgctcac tggacctgcc gcagtcactc acgatgaagt ggcagctgct ctctccagcg
     2101 tcagcggaaa aagcataaca tacgtaaacg taacgcctga ggacttcaag aaaggtcttc
     2161 tctctggcgg agttccaaac gattacgcgg acttcctggt tttcattgca gggaacctca
     2221 gggagggaca tgcatccccg gtcacagcaa ccgtgaagca aatcacggga aaagagccga
     2281 ttgcgttcca acagtatgca aacgacaacc aggctgcctg gaaataacct gagaaacgat
     2341 gccgtggcac ccgccacggc ttgaattttt ttctagacag aatccacctt ccactccgga
     2401 ttagacagat cggtctaatc ccggaggcgg aacatgaagc gactcgtttt ccagaaaaaa
     2461 ggaatcattg attgggaaga atgtccggca cctgtcatca cagggcccaa ccaggccatt
     2521 gttcggccgc tggcgattgc tcgctgt
//
LOCUS       DAOQJL010000558         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_29445_length_2544_cov_4.168341, whole genome shotgun sequence.
ACCESSION   DAOQJL010000558 DAOQJL010000000
VERSION     DAOQJL010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29445_length_2544_cov_4.168341"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLG78_17915"
     CDS             complement(<1..252)
                     /locus_tag="PLG78_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25055.1"
                     /translation="MVVVLTIFLAIFADGCSRYAQDCAIMDARCNAAIFALAFRRLTE
                     SRFAYVAVTAGTIYEYSVSRTNGTLTQIGLIFGVLIANTV"
     gene            401..>2544
                     /locus_tag="PLG78_17920"
     CDS             401..>2544
                     /locus_tag="PLG78_17920"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE25056.1"
                     /translation="MPGVRLKKDQMAWICDPAWIGFDTSDQIPDGPGTFGQNRARDAI
                     EFAANMGRPGYNTAVVGEPGSGRHFLARRLLEKRAATEAPPDDWCYVNNFEDSTRPRV
                     LMLPAGRGAGLKADMHRFAAELGSTMSSAFEAEEYRRHIEQIQNELKAREETALRELG
                     ASSLKEGIALISTAQGFVFTAVKNSEPLAPEEFQKLPEEDQRAISEKIEKFGTTLQEV
                     LRQFPVWRRETQNRLRDVMRNTLGLAVGHIIDEIKDKFRDLPEVIAFLDQVLSDVVDS
                     GPQARDAKEEEGSTISGVPAPFQKYEVNLLVDRDGQKCAPVVYEDNPSYQNLIGRIDQ
                     VGHFGLLVTNFTMIRAGSLHRANGGYLLVDGEKLLAQPFAYDSLKRALKSSTIRIESP
                     GQLLAGISTSPLEPDTIPLRVKVVLFCGRQLHYMLRELDPEFDELFKVAAEFESDVER
                     TPETVSLYAQMLASLVRTEKLRSFTREAFARIIEHSARLAGDANRLSARTRRLADLMR
                     ESDYFAGQAGRASVDVQDVMRSLKEAEDRRGRIREAIQREILRDTIQIATNGAAVGSI
                     NGLAVAIQGDAAFAHPVRITAAVWPGKGDIIDIERETELGGQIHSKGVFILSAFLASR
                     FSRTKPLSLSASLVFEQSYGPVEGDSASLAELCVLISAISGVPILQGIAMTGSVSQHG
                     VAQAIGGASEKIEGFFDICRARGLTGSQGVIIP"
BASE COUNT          616 a          650 c          693 g          585 t
ORIGIN      
        1 cacggtgttg gcgatcagca cgccgaagat caggccgatc tgcgtgagcg tgccattggt
       61 tcttgataca gaatactcgt atatggttcc ggctgtcacg gccacatacg caaaacggga
      121 ttcagtgaga cgtctaaaag caagcgcgaa gatcgcagca ttgcatctcg catccatgat
      181 cgcgcaatcc tgagcgtagc ggctgcagcc atctgcaaaa attgccagaa agatagttag
      241 aacaacaacc aatcgttgca acatatatag tatatttacg aatcgaatct gattttaagg
      301 cgaatatttt gttagtcgcg accatttcat ggcaccgacc tgcggtcgca gaggcgtgag
      361 cttgccttga cgcgcagctc gtgctgacaa agatcatact atgcccggtg tgagactgaa
      421 aaaagaccag atggcctgga tttgtgatcc agcgtggatt ggctttgata cttcggatca
      481 aattccagat ggtcccggga ctttcgggca gaaccgtgcg cgcgatgcaa ttgagtttgc
      541 ggccaatatg ggtcgaccgg gttacaacac ggcggtggtt ggagagccag gaagcggcag
      601 acattttctt gcgcgcagac tccttgagaa gcgcgcagca acagaagcac ctcctgacga
      661 ttggtgctac gtaaataatt tcgaggattc tactcggccc agggttctca tgcttcctgc
      721 gggcagggga gccggactca aagcagacat gcatcggttt gcggctgaac tcggttccac
      781 tatgtcttct gcctttgaag cggaagagta ccgccgtcac attgaacaga ttcaaaacga
      841 actcaaagcg cgcgaagaaa cagcattgcg cgaactgggg gcctcctccc tcaaagaagg
      901 aatcgctctg atcagcactg cgcagggctt tgttttcacg gcggttaaga attcagagcc
      961 tcttgctcct gaagaatttc agaagctgcc cgaggaagac cagcgggcga tcagcgaaaa
     1021 aatagagaag ttcgggacta ccttgcaaga ggttctgcgc cagtttccag tatggcgtcg
     1081 cgaaacacag aatcgccttc gcgatgttat gcgaaatacg ctcggtctgg ctgtaggtca
     1141 tatcatcgat gaaatcaagg acaagtttcg cgatttgcca gaagtcattg cttttctcga
     1201 tcaggttctt tcggacgtag ttgacagcgg tccacaggca cgcgacgcaa aagaagagga
     1261 agggtctact atttctggcg tgcctgctcc ttttcagaaa tacgaagtca atcttctagt
     1321 ggatcgcgac ggacagaaat gtgcccccgt ggtttatgaa gacaatccat cctatcagaa
     1381 cttgattggg cgaattgatc aggtgggtca ttttggtctt ctggtgacca acttcaccat
     1441 gattcgtgcc ggctcccttc acagagcaaa cggcggttat ctgcttgtgg atggagaaaa
     1501 actcctggct caaccattcg cctacgacag tcttaagaga gcactcaagt cttctacgat
     1561 tcgaattgaa tctcccgggc agcttctcgc tggaatttct acttcacctc tagagccgga
     1621 caccattcct cttcgcgtga aagttgtgct gttctgtggg cggcagcttc actacatgct
     1681 gcgagaactg gatccggaat ttgacgaatt gttcaaggtg gcggcggaat ttgaaagtga
     1741 tgtggagcga acgcccgaaa ccgtctccct ctacgcgcaa atgcttgcat cccttgttcg
     1801 cacagaaaaa ctcagatcgt tcacacgcga agcgttcgct cgtatcattg aacacagcgc
     1861 caggctggcc ggggacgcaa acagactcag cgcgcgcacg cgtcgtctgg cggatctgat
     1921 gcgcgaatca gactacttcg cggggcaggc aggcagagct tccgtagacg tacaggacgt
     1981 tatgcgatct ctgaaagaag cggaggaccg tcgcggtcga atccgcgagg caatccagag
     2041 agaaattctc cgtgatacaa tccagatcgc cacaaatgga gccgcagttg gttctatcaa
     2101 cggtctggcg gtcgcgattc aaggagatgc tgccttcgcg caccccgtgc gaattaccgc
     2161 cgcagtgtgg cccggaaaag gagacatcat cgacattgag cgcgaaacgg aactgggcgg
     2221 acagattcac tcgaaaggag tattcattct ctccgctttc cttgcttcca ggttttcgcg
     2281 cacgaagcct ctatctctct ccgccagtct tgtattcgaa cagtcctatg gccctgtaga
     2341 aggggactct gcgtccctgg ctgaattgtg cgttctgatt tctgccatct ccggagttcc
     2401 gatccttcag ggaatcgcca tgaccggttc ggtgagccag cacggtgtcg cccaggcaat
     2461 tggaggggcc agcgaaaaaa tagaaggctt ctttgacatt tgcagggcga gaggtctcac
     2521 aggcagtcag ggagtgatca ttcc
//
LOCUS       DAOQJL010000559         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_29522_length_2540_cov_4.512274, whole genome shotgun sequence.
ACCESSION   DAOQJL010000559 DAOQJL010000000
VERSION     DAOQJL010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29522_length_2540_cov_4.512274"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1471
                     /locus_tag="PLG78_17925"
     CDS             86..1471
                     /locus_tag="PLG78_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNE25057.1"
                     /translation="MKHFAQILAPGGAISADRLRQTASICLQADVRSIEIGPRMNPVI
                     PAGAGVSKILARIDGIDFKMAPNVISSAPAAGFDSSNWVRESQLLEMAEEVASLNPHV
                     SISLIDAQQGLVGRTLSELNFVSCEQDEFWQPVLAPEGKRIDLGSRIATGDVVGAARW
                     YSASKKGGTDALTAFFRSTMTRSSMPAEPLPPEPSAPPYEGLSAKRGTLGFFVSSGQY
                     DCEMLEELAYLCSQSSIGKIHMTPWHSFLIKGIQEKQLDLWRNFLLRKGIETRHAGSS
                     LYWRFSNGRSNQSNRIHRKIIHQLGSTDKSTGQIPFVVGKDLEGQPALLGIEPTSLFR
                     CIIYKRDNLNEPWDKHGTFLEAFAAREIQIICDQRARNQKQRSGENAAESDSVHAQPD
                     VKASNAELNPGVREPGRSGEFVCTLCGNVYSPEVGEPFTQIAPGLAFEDLPESYACPV
                     CGSGKSEHTAA"
     gene            complement(1475..>2540)
                     /locus_tag="PLG78_17930"
     CDS             complement(1475..>2540)
                     /locus_tag="PLG78_17930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE25058.1"
                     /translation="EEEFARSVGADHAIAVNSATAGLHLGLQVLGIGKGDLVVLPSIT
                     FTATAEAVLYTGAKPAILDVGRSDYLLNAEVILKFIQDRCETREGKIFEKASGLRLKA
                     IMPVHLGGRPCDLDAIQAIADKHDLHVVEDAAHAFPAKYKNRMIGNISDMTAFSFYAT
                     KNLATGEGGMITTNNEKAAGRLRRLRLHGIKGQTWGRKRWKYDVVEQGYKYNMTDLAA
                     AMGRVQLARMNEMQERRMSIAARYREAFADIKGIQIVPDPDGSSQHLFTIEIGKEAPV
                     SRDRFVEEMYARGIGTSLHFIPLYRHTFYKEMGYSPADFPASEEIYRRIVSLPLYSAM
                     TDDDMEDVVFAVRETFAISR"
BASE COUNT          636 a          654 c          647 g          603 t
ORIGIN      
        1 gaagctgtcg gcctgaagtg gccaggattc tggcggcgtc tcttgaaaat gaaaaagcac
       61 caaaggaaaa cgaggagaga gttgcatgaa gcactttgca cagattcttg ctccgggtgg
      121 tgcgatctct gcagatcgtt tgagacaaac ggcaagcatt tgtctgcagg cggatgtacg
      181 ctcgattgaa attggaccaa gaatgaaccc agtcataccg gccggtgccg gtgtctcaaa
      241 aattcttgca aggatagacg ggattgattt taagatggcg cctaacgtca tctcttctgc
      301 gcctgctgcg ggctttgact cctcaaactg ggttcgagaa agtcagcttc tggaaatggc
      361 agaagaagtg gcctctttga atccgcacgt gagtatttcg ctcatagatg cacagcaggg
      421 tctggtgggc agaactctga gcgaattgaa cttcgtttct tgtgagcagg acgagttctg
      481 gcaacctgtg cttgctccag agggaaaaag gatcgatctg gggagcagaa tcgcaacagg
      541 cgatgtggtt ggcgcggcac gctggtattc cgcctctaaa aagggaggca cagacgcact
      601 cacggcattc tttcgaagca cgatgacccg atcctccatg cctgccgaac ctctccctcc
      661 tgaaccctcg gcgccaccat atgaaggcct cagcgcgaaa cgtggcacac tcgggttctt
      721 cgtctccagc ggacagtacg actgcgagat gctcgaagag ctcgcctatc tttgcagcca
      781 gagcagcata ggcaagattc atatgactcc ctggcactcc ttcttgatca aagggataca
      841 agaaaaacag ttagacctct ggcgaaattt tcttttgcga aaaggaatcg aaactcgcca
      901 cgcaggttct tctttgtact ggagattctc aaacggcaga agcaatcaat ccaatcgcat
      961 tcacagaaag atcattcacc agcttggatc cactgacaaa tcaacagggc agattccatt
     1021 cgttgtggga aaagacctgg aaggacagcc ggcactgctt ggaatcgagc caacgagttt
     1081 gtttcgctgc atcatttaca agagagacaa tctaaacgaa ccatgggaca agcacggtac
     1141 attcctcgaa gcattcgcag cacgcgagat tcaaatcatc tgtgatcaaa gagctcgcaa
     1201 tcaaaagcaa cgatccggag aaaatgcagc agaaagcgat tccgtgcacg cacagcccga
     1261 tgtcaaagcg tccaatgctg agttgaatcc gggtgtaagg gaacctggaa gaagtggcga
     1321 atttgtctgc accctctgcg gcaacgtata tagccctgaa gtcggtgaac cgtttacgca
     1381 gattgcgcct ggactcgcat ttgaggatct tccggaatcg tacgcatgcc ctgtgtgcgg
     1441 atcaggtaag tccgaacaca cagcggcttg atttctacct ggaaattgcg aacgtttcgc
     1501 gcactgcgaa cactacatct tccatgtcat cgtccgtcat agcgctgtaa agaggcagag
     1561 atacaatgcg tctgtaaatc tcttcgctcg cggggaaatc cgcgggtgaa tagcccattt
     1621 ccttgtagaa agtgtggcga tagagcggaa tgaaatgaag gctcgttcca atcccgcggg
     1681 cgtacatctc ttcaacgaat cggtctcgac tgaccggcgc ttccttacca atctcaattg
     1741 taaagagatg ttgagaagac ccatcgggat cgggtacgat ttggattcct ttgatatccg
     1801 cgaaggcctc gcgatagcga gctgcaatcg acatacgtct ttcctgcatc tcgttcatgc
     1861 gcgctagctg gacacgaccc atggctgccg cgagatccgt catattgtac ttataaccct
     1921 gttccaccac gtcatatttc cagcgtttgc gaccccaggt ctggcctttg atgccgtgga
     1981 gacgaagtct gcgcagtctt ccggccgcct tctcattgtt agtcgttatc attccgcctt
     2041 cgcctgtggc caggttcttg gtggcataga agctgaatgc ggtcatatcg ctgatgttgc
     2101 caatcatgcg attcttgtat tttgctggaa atgcatgagc cgcatcttcc actacatgca
     2161 agtcatgctt atccgcgatc gcttgaatgg catccaggtc gcaaggtctt ccgcccagat
     2221 gcacaggcat gatcgccttg aggcgaagac cggaggcctt ttcaaaaatt ttcccttcgc
     2281 gcgtttcgca tcgatcctga atgaatttca gaattacttc agcattcaac agataatctg
     2341 atcttccgac gtccagaatc gcaggtttcg cgccagtata gagaactgct tctgcagtcg
     2401 ctgtaaatgt aatcgaagga agtactacga gatctccttt cccaattcca agaacctgga
     2461 gtccaagatg aagaccggcc gtggccgaat ttacggcgat cgcatgatct gcaccaacgg
     2521 atcgcgcgaa ttcttcttcg
//
LOCUS       DAOQJL010000560         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_29911_length_2521_cov_4.094891, whole genome shotgun sequence.
ACCESSION   DAOQJL010000560 DAOQJL010000000
VERSION     DAOQJL010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29911_length_2521_cov_4.094891"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLG78_17935"
     CDS             complement(<1..939)
                     /locus_tag="PLG78_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNE25059.1"
                     /translation="MIAVCFYFEVHQPFRLRPYHFFDVGVDPHYLDEQKNRDICRKVA
                     NKCYLPANKVLLDLIKLYGSDFRVSFSITGTAIEQFRMYSPETLDSFRKLADTGQVEF
                     LNETYYHTLASLYSENEFRAQVAKHGQLMREEFGYNPVAFRNTELIYSNHIAWLAREM
                     GFQTILLEGIERVLDWRSPNHVYAPEHSPGIKCLLKNYRLSDDVAFRFSDRNWKDWPL
                     TAEKFAGWVHQVAGNGTTINLFMDYETFGEHQWEDSGIFSFLKELPGRILRHPDFCFR
                     TVTQVSRAHQPVGTIDAHSPISWADIERDLSAWMGNS"
     gene            complement(936..2231)
                     /locus_tag="PLG78_17940"
     CDS             complement(936..2231)
                     /locus_tag="PLG78_17940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE25060.1"
                     /translation="MSNYKIFMIGWEYPPRITGGLAVACEGIASSLGRRGNSVRFLVP
                     RMHGDEPVHENVELVDAGTRTELYTTDELEVLRSMALHDFTTRQVFSPYASSEYSGQS
                     LVDITEKLAVMQKQSREIPLLRGGYGDWMYQEIERYARAAYFIAQKTEMDVIHAHDWM
                     TFPAGLACMNATGAPLVVHVHATEFDRSGESVNQYVYDLERYTFHHATAIMTVSNYTK
                     NVLVQRYGVSPEKIFPIHNAVEFELPGAVLERKKALKDHIVLFLGRITFQKGPDYFVR
                     AAQLVLQQIQNVRFVMAGTGDMYSRMIEYAADLGIGKFFHYTGFMNRAQVKRLYSMSD
                     LYVMPSVSEPFGISPLEAMMHGVPVIVSRQSGVSEVIQNCIKVDFWNVEELAEQILRV
                     LHDTELKQNMQQEGHREVQAISWDAPAEKMEQVYGRLVK"
BASE COUNT          591 a          600 c          623 g          707 t
ORIGIN      
        1 gctgtttccc atccacgcgg atagatcgcg ttctatgtct gcccacgaaa taggtgaatg
       61 agcgtcaatg gttcctaccg gctggtgtgc ccttgaaacc tgggtcacag tgcgaaagca
      121 gaagtcagga tgtctgagaa ttcttcctgg caattctttg agaaaactga agatccccga
      181 atcctcccac tgatgttctc cgaaggtttc atagtccatg aagagattga tcgtagtccc
      241 gttgcctgcc acctggtgaa cccagcctgc gaacttctct gcggtcaaag gccagtcttt
      301 ccagtttctg tctgaaaatc gaaacgcaac atcatcgctg agtctataat tcttgagaag
      361 gcatttgatt ccaggactgt gctcaggcgc gtacacatga tttgggcttc tccagtcgag
      421 cactcgttcg attccttcga gaaggatggt ctgaaatccc atttcgcgcg caagccacgc
      481 aatgtgattg gagtagatga gttctgtgtt tcgaaatgca actggattgt atccgaattc
      541 ttcgcgcatg agctggccat gctttgccac ctgagcgcga aattcgtttt cagaatacag
      601 agatgcgaga gtgtgatagt aagtttcgtt tagaaactca acctgtccag tatctgcgag
      661 tttgcgaaag ctatccaggg tctcgggcga atacattctg aactgttcga tcgcagttcc
      721 tgtgatggag aatgaaacac ggaaatcaga accgtagagc ttgattagat ccaggagtac
      781 tttgtttgca ggaagatagc acttgtttgc cactttgcgg catatatcgc ggtttttttg
      841 ttcatccaga tagtgaggat ccacgcccac atcgaagaag tggtaggggc gaagtctgaa
      901 tggctggtgt acttcgaaat agaagcacac tgcgatcatt tcacaagtct cccgtacacc
      961 tgctccatct tttcagcggg agcgtcccag gaaattgcct gtacttctct atggccttct
     1021 tgctgcatgt tttgcttgag ttccgtgtcg tgcaggactc gaaggatttg ttctgcgagt
     1081 tcttctacgt tccagaaatc tactttgatg caattttgaa tgacttcgct tacacctgac
     1141 tgcctggata cgatgactgg aacgccgtgc atcattgctt ctagaggaga gattccaaat
     1201 ggttcggata cagatggcat cacgtaaaga tcgctcatgg agtagagtct ctttacctgc
     1261 gcgcgattca tgaatccagt gtagtggaag aacttaccaa tccccaggtc tgctgcatat
     1321 tcgatcatgc gtgaatacat gtctcctgtg cctgccatta caaatcgcac attctggatt
     1381 tgctgaagca cgagttgcgc tgcacgaacg aagtaatccg gtcccttctg gaatgtgatt
     1441 ctacccagaa acaaaacgat gtgatccttg agtgctttct ttctttcgag gaccgcgccc
     1501 gggagttcaa actcaactgc attgtgaatg ggaaatattt tttccggcga aacgccgtat
     1561 ctttgcacca gcacattctt tgtgtaattg ctcacagtca tgatggcagt tgcatgatga
     1621 aatgtatatc tttcgaggtc gtatacgtac tggtttacac tttctccgct tctgtcaaac
     1681 tcagtggcgt gcacgtgaac gaccagagga gctccagtag cattcataca cgcgagaccc
     1741 gcaggaaagg tcatccagtc atgagcgtga atgacatcca tttctgtttt ttgtgcgatg
     1801 aagtacgcgg cgcgtgcgta tcgctcgatt tcctggtaca tccagtcccc gtatcctccg
     1861 cgaaggaggg ggatttcgcg ggattgtttt tgcatgaccg cgagcttttc tgtgatgtcc
     1921 accagagatt ggcctgaata ctccgaggat gcgtaggggg aaaagacctg tctggttgtg
     1981 aaatcatgca gcgccatgct gcgaagcact tccagttcgt ctgtagtata gagttctgtt
     2041 cttgtacccg catctacgag ttctacattt tcatgaactg gttcgtctcc atgcattctg
     2101 ggtacgagaa agcgcacact gttccctcgt cttcccagag acgatgcgat tccttcgcat
     2161 gcgacggcca gtcctcccgt gattcggggg ggatactccc agccaatcat gaagatcttg
     2221 tagttgctca ttagccgatt ccaggcccgg tgcgcagcat atagaacccc ttttcgtttt
     2281 ctatttgatg tttacaacaa ttatcataga atggggatga attccatgct ttttcaaagg
     2341 agtgtacggt gataaaagtc atggccggtg agccttatcc actcggagca acctgggatg
     2401 gatctggcgt aaatttctct ctgtactccg aacatggaac cggcgtagag ctgtgtctat
     2461 tcgattccgc tgatgcgaag aaagagagtc gtagaatttc tatgacgcac cgcacagata
     2521 t
//
LOCUS       DAOQJL010000561         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30043_length_2515_cov_4.187398, whole genome shotgun sequence.
ACCESSION   DAOQJL010000561 DAOQJL010000000
VERSION     DAOQJL010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30043_length_2515_cov_4.187398"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLG78_17945"
     CDS             complement(<1..750)
                     /locus_tag="PLG78_17945"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNE25061.1"
                     /translation="MAIEETRVVGPWEQMKNSFGGMLIGLLLLPGAFFIVYISTCREQ
                     ASVEMKGALPASEAAKAAADNKPVYATGKLDSSPVGDPQFVKPGKYLKLSRSAQIMAW
                     VHKETTKTEGSGTTKKETKTYDCKLEWTSSPDLNVGSKKGCEGKTNFSATYSSADFAA
                     DSVTINSSGTTYSLNPSAIDPVKLPGAKVQKSDLTRDMTEYSGAFYPEAACATSSPKA
                     GCEKITFSGTSYEAGKDYTAVGKLVGNQFEEY"
     gene            complement(858..>2515)
                     /gene="lon"
                     /locus_tag="PLG78_17950"
     CDS             complement(858..>2515)
                     /gene="lon"
                     /locus_tag="PLG78_17950"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNE25062.1"
                     /translation="KEKWLPDDVRKAVDKELKRFRRAQEGPQGGGFEVNQIRNWLELI
                     LELPFENPEPREILLSRAEEILNEDHEGLEEVKKRILEYLAVEKQTGGSRAPILCLVG
                     PPGVGKTSLARSVARALGRPLVRSALGGVRDEAEIRGHRRTYVGAMPGKILTAMKKAE
                     RRDAVFLLDEIDKTGTSYHGDPSAALLEVLDPEQNHSFEDHYLGLPYDLSRVLFVATA
                     NNADSIPLPLLDRMEIVNLSGYTLAEKRQIARKHLVPTILNDLKLDADQISFTDGAIE
                     KVITAHTREAGVRSLKRKLEGIARKSIRKILEAREKNQSTGNEEKLTIREEDVKEMLG
                     RDRFHRDMKEHLGKPGLAVGLAWTPVGGDILYIEAASYPGKGDFKLSGQLGDVMKESA
                     HAAFSFLKSRAQEIGISQEAFATRDFHIHIPSGAIPKDGPSAGVTLLSALTSLLKGVP
                     IAENIAMTGEISLRGRILPVGGIKEKVLAARAAGIETVLLPEQNRPEFEEIPAHVTEG
                     LRVEYYSEMMELLKRTVPRAFESNTGSAGQTGYSMQASRRDEW"
BASE COUNT          506 a          664 c          631 g          714 t
ORIGIN      
        1 atactcttcg aattgatttc cgaccagttt acccactgca gtgtaatctt ttcctgcttc
       61 ataggaagta ccggagaagg tgatcttttc gcatccagct ttaggactgg atgtggcaca
      121 ggccgcttca gggtagaagg cgccgctgta ttcagtcatg tcgcgcgtta gatctgattt
      181 ctgtactttt gcgccaggca gtttgactgg atcaatggca gatggattga gggagtaggt
      241 tgtgccggat gaattgatgg tcactgagtc tgctgcgaaa tcggcgctag aataagttgc
      301 ggagaaattt gttttccctt cgcatccttt cttgctgcca acattcaaat caggagagct
      361 ggtccattcc agtttgcagt cgtacgtctt ggtctctttc ttggtagtgc cgctgccttc
      421 tgttttcgtg gtttctttgt gaacccacgc catgatctgc gcacttctgg agagtttgag
      481 atacttgcca ggttttacaa actgcggatc acctactggt gatgaatcga gtttgcctgt
      541 cgcatagact ggcttgttgt ctgccgcggc tttcgctgct tctgaagcag gaagtgcccc
      601 tttcatttcc acagaggcct gttccctgca cgtggatatg taaacgataa agaatgcgcc
      661 cggtaacaga agcaggccaa tcagcatgcc cccgaatgaa ttcttcattt gctcccaggg
      721 cccgaccacc cttgtttctt ctattgccat gattgcctcc gcacgaatgt ctgcggaaaa
      781 tgcgcgacat ggagcagaga ttcaactaaa atttgttttt tgagaatgtg cgttgcacca
      841 ggcttcgcac actttcttta ccattcgtct cgtcttgagg cctgcatgga gtaaccggtc
      901 tggcccgctg agcccgtgtt tgactcgaag gcgcgaggaa ctgttcgctt caacagttcc
      961 atcatctcag aatagtactc cactcgcaga ccttctgtca cgtgcgctgg aatctcttca
     1021 aattcagggc gattctgttc tggcagcaga acggtttcta ttcctgccgc acgcgctgcg
     1081 aggactttct ctttgatccc acccaccgga agaattcgcc cgcgaaggga gatctcgcct
     1141 gtcatcgcaa tgttctcagc aatcggcact cctttgagta gactggtcaa tgcgctgaga
     1201 agtgtgacgc ccgccgaagg tccgtctttt ggaatcgctc cactcgggat gtgaatatgg
     1261 aagtctctgg ttgcgaaagc ttcttgcgag atcccaatct cctgcgcgcg gctcttgaga
     1321 aaagagaatg cggcgtgcgc agattctttc atcacatcgc ccagctgtcc ggagagtttg
     1381 aagtctcctt ttccaggata cgaagccgct tctatataga gaatgtctcc gcccacaggg
     1441 gtccatgcaa ggccgaccgc aagtcctggc ttcccgagat gttctttcat gtcgcggtgg
     1501 aagcgatccc ggcccagcat ttctttgacg tcctcttcgc gaatggtcag cttctcttcg
     1561 ttgccggtag actgattctt ttcgcgcgct tctagaatct tgcgaatcga tttgcgtgcg
     1621 atcccttcga gttttcgctt gagggaacgg actcctgctt cacgagtgtg cgccgtgatt
     1681 actttttcaa tggctccgtc cgtgaacgat atttgatctg cgtcgagttt cagatcattg
     1741 agtatggttg ggacaagatg ctttcttgca atctgacgct tctctgctag agtgtagccg
     1801 gacagattca cgatttccat tctatcgagc aatggaagcg gaatgctgtc cgcgttgttc
     1861 gcggttgcta caaacagaac acggctcaaa tcgtacggaa ggcccaggta atgatcttca
     1921 aagctgtgat tttgctctgg atccagtact tcgagaagtg ccgcagatgg atctccgtga
     1981 taggatgttc ctgttttgtc gatttcatca agtaagaaca cagcatctct gcgctctgct
     2041 ttcttcatcg cagtcaggat ctttccaggc atcgcaccta cgtacgttct tctgtgaccc
     2101 cggatttccg cttcatcgcg cacgccgccc agagcggagc ggaccagcgg tctaccgaga
     2161 gcacgtgcca ctgatcgtgc cagagaagtt tttcctaccc ctggaggacc cacgaggcaa
     2221 agaatcgggg cgcgcgagcc acctgtttgt ttctctaccg cgagatattc aagaatccgc
     2281 tttttcactt cttcaaggcc ttcgtggtct tcgtttagaa tctcttctgc gcggctgaga
     2341 agaatttcac gtggttctgg attctcaaaa ggcaattcca gaattagttc cagccagttg
     2401 cggatctggt tgacttcgaa tcctccaccc tgcgggcctt cttgcgcgcg gcggaatcgt
     2461 ttcaattctt tatcaaccgc tttgcgcaca tcgtctggca gccacttctc tttca
//
LOCUS       DAOQJL010000562         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30278_length_2505_cov_3.573878, whole genome shotgun sequence.
ACCESSION   DAOQJL010000562 DAOQJL010000000
VERSION     DAOQJL010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30278_length_2505_cov_3.573878"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="malQ"
                     /locus_tag="PLG78_17955"
     CDS             complement(<1..1548)
                     /gene="malQ"
                     /locus_tag="PLG78_17955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE25063.1"
                     /translation="MQPAESALDILSRRAGISLEYHDIDGVAHTTSEEAKRTILEILG
                     YSGDPSAILSNLIQADATTLIAPAVIASVYAPRFNMRIPVDIVGQIELRIELEGGITV
                     GLRPQFELKEEHLVAGRTIGLYEVDLKDSIPPGQHIISVMRWKEEIGRSTLFLCPESC
                     YLNAEIENGSRWNGLWLQLYALRSSANWGIGDLGDLLELARLVGPRGIRILGLSPLHA
                     PFLMRPDFKSPYSPSSRLYKNAMFVSIPLLPEFAASAKARSLFESSEFQLYLARTRPE
                     ERIDYAGVYQWKMRFFEILFDEFISDPPEDFAEYVEEGGIELSRQAIFDCLEERFDYN
                     QDRPAGFHGWPEAFQDASSDAVRAFADASHRRVQFYIFLQYLFDRQFSDVSGACLDLG
                     VCLYADLAVGSHPAGAEVWSSPDAFVERASVGAPPDPFAPQGQDWGLPPLHPRKLVDT
                     RYAAFVDLFERNMPYGGLVRIDHVMALCRLYWVVSTSAGKTGAYVEYPFVELLRLAAL
                     VSQRKRCM"
     gene            complement(1550..>2505)
                     /locus_tag="PLG78_17960"
     CDS             complement(1550..>2505)
                     /locus_tag="PLG78_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25064.1"
                     /translation="WDLGEGGYQVGNFPVQWTEWNGHYRDCVRSFWIGGNVAREFATR
                     LTGSSDLYEAAGRRPYASINFVTCHDGFTMLDLVSYDRKHNEANGWNNTDGNDHNISF
                     NHGTEGETQDPTIRATRFRQMRNYFATLLLSQGVPMINMGDELARTQKGNNNAYCQDN
                     DISWIDWDLTEEQKHHLEFVKKLIHLRRQSRVLRRNHFIRGGEPGEERDMIWLHPDGH
                     EMKSSEWYDSMPIFGAIIDGLHPQENKKFYEIGGKDEDVEPLLIVIARSAGEFRIPAE
                     PNLSWYRILDSELGPSISVKAGTSLDLMALTVSVFRPDRRG"
BASE COUNT          620 a          673 c          604 g          608 t
ORIGIN      
        1 catgcatctc tttctctggc tgacgagggc tgccagtctc aacagttcta caaacggata
       61 ctctacgtag gcgcccgtct ttcccgcgga agtggatact acccagtaca ggcgacagag
      121 agccatcacg tgatcgattc gcacaagccc gccgtagggc atgtttctct caaacagatc
      181 aacaaatgcc gcataacggg tatctacgag tttcctggga tgaagaggag gaaggcccca
      241 gtcctggccc tgcggcgcaa atggatcggg tggcgcgcct acagaggctc gctctacaaa
      301 tgcgtccgga ctgctccaga cttctgcgcc cgcaggatgc gatccaactg cgagatccgc
      361 atacaaacac acgcctagat ccagacatgc accggaaaca tctgaaaact ggcggtcgaa
      421 caaatattgg agaaatatgt agaattgaac tcttctgtgg cttgcatctg caaatgcgcg
      481 caccgcatca ctcgatgcgt cctgaaaagc ttctggccat ccgtgaaagc ctgccggcct
      541 gtcctgattg taatcaaacc tttcctccag acaatcgaag atcgcctgac gggagagttc
      601 tatgccaccc tcctccacgt attcggcaaa gtcttccgga ggatctgaaa tgaattcgtc
      661 gaagagaatc tcaaagaacc gcatcttcca ttggtacacg ccggcgtagt caatgcgctc
      721 ctctggcctg gtccgggcaa gatacagctg aaattctgaa ctttcaaaca agctgcgtgc
      781 ctttgcagat gctgcgaatt caggaaggag cggaatagaa acgaacattg catttttgta
      841 gagtctgctc gaaggagaat aaggactttt gaaatccggt cgcatgagga acggagcatg
      901 cagaggggaa aggccaagga ttcggatgcc gcggggccca accagacgcg cgagttcgag
      961 caaatctccc agatccccaa ttccccagtt tgcgctcgat cgaagtgcgt aaagctgcaa
     1021 ccacagaccg ttccacctgg atccattttc aatttccgca ttcaaatagc aggattcagg
     1081 gcacagaaac aaagtcgatc gccctatctc ttctttccat cgcataacac tgattatgtg
     1141 ctgtcccggt ggaatagaat ccttgagatc tacttcgtat agaccaatcg tgcgaccggc
     1201 cacaagatgc tcttctttta actcaaactg cggtctgagg ccaactgtga ttccaccttc
     1261 gagttcaatg cgcagctcaa tctggccaac tatgtccacc ggaatccgca tgttgaatct
     1321 gggcgcatag acggacgcaa tgacagcggg cgcaatgaga gtggtggcat ctgcttgaat
     1381 caggtttgaa aggattgcag atggatctcc gctgtaaccc aggatctcaa gaatggttcg
     1441 ctttgcttct tctgaagttg tgtgagcgac tccgtcaatg tcatgatact caagcgaaat
     1501 ccccgctcgc ctggacagaa tatcaagtgc agactctgca ggctgcattt caacctcttc
     1561 gatccggtct aaagacagaa actgtaagag ccatcaaatc cagagacgtt ccagccttca
     1621 cagaaatgga aggaccaagc tcagagtcga ggatcctgta ccatgaaagg ttgggctccg
     1681 cgggtatccg aaactcgcct gcgctgcgcg cgatgacaat caagagaggt tccacatctt
     1741 catctttgcc acctatttcg tagaacttct tgttctcctg cggatgaaga ccatcaatga
     1801 tcgcaccaaa aatgggcatg gaatcatacc attctgaact cttcatttcg tgcccgtccg
     1861 gatgaagcca gatcatgtct ctctcttctc ctggttctcc tccgcgaatg aaatgattcc
     1921 tgcgaagcac tctactctgt ctgcgtaaat ggatgagttt ctttacgaat tccagatgat
     1981 gcttttgttc ttctgtcaga tcccagtcaa tccaggaaat gtcattgtcc tggcaatacg
     2041 cgttgttgtt tcctttttga gtgcgggcca gctcgtctcc catgttgatc atgggtactc
     2101 cctgcgaaag aagtagagtt gcaaagtagt ttctcatctg gcgaaagcga gtagcgcgaa
     2161 tggttggatc ctgcgtctct ccttctgtgc cgtgattgaa actgatgttg tgatcgtttc
     2221 cgtccgtgtt attccagccg tttgcttcgt tgtgttttct atcatagctg accagatcga
     2281 gcatggtgaa tccgtcatga caggtaacaa agttaatact tgcatacgga cgccgaccgg
     2341 ccgcttcgta tagatcgctg gaacctgtaa gacgagtggc aaattcgcgc gctacattcc
     2401 caccaatcca gaaactgcgc acacagtcgc gatagtgtcc gttccactcc gtccattgaa
     2461 caggaaaatt gcccacctgg tagccgcctt ctcccagatc ccagg
//
LOCUS       DAOQJL010000563         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30318_length_2503_cov_3.837418, whole genome shotgun sequence.
ACCESSION   DAOQJL010000563 DAOQJL010000000
VERSION     DAOQJL010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30318_length_2503_cov_3.837418"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLG78_17965"
     CDS             <1..196
                     /locus_tag="PLG78_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25065.1"
                     /translation="YLAEMMIGILRFHMRCIEIPVSLYRRYGSVRTETTSEQWQYFFS
                     VLRLIFGRRFTLLGKLFRVS"
     gene            255..1661
                     /locus_tag="PLG78_17970"
     CDS             255..1661
                     /locus_tag="PLG78_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA domain protein"
                     /protein_id="HNE25066.1"
                     /translation="MKKILYFVVIPLFVILALVSASAWYALKVVLNENFLVQQIESNL
                     NVRAEVRKLNVSLFSAMSSVELEGVSLGARDSFANQGTPLSERPPMKGSVVTIGKIDL
                     KFSLLPLLRRKFELNRFMLSSPVVSMTLFANGGNSLKPLFQKPLIVGRKPNPALAEKP
                     AETAAKEEEKPFSAKDLPISADLREIGLKDATVNLLMQKTGQLIRISGLALVIDSIDI
                     DPADLVKHNNAHLDFDANVNVISAKQTDAASFLIRSSGNVTPFDSKTGEVSTGVEYAL
                     VVKKDSFITGMAIFDQLAGELPVLSQVGFSWKKLSEKAVLLRDVNVRVRYSHALVTFL
                     DDTVFPTANYDLLLKKGSTINCATNEHNFNASVRATEAESKEILARFDRGIEKKLKGQ
                     SVEDVRNRVLGKLIQNGQVDLPFASLGNFANPSVKLLAELPSLTEVLTGSVKDILKNK
                     LDQKIPGVGKELLNKLPF"
     gene            complement(1712..>2503)
                     /locus_tag="PLG78_17975"
     CDS             complement(1712..>2503)
                     /locus_tag="PLG78_17975"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNE25067.1"
                     /translation="VYFFPSSHAIESMYGLAVILTMNIGIFAYLLRQIRAHKINGMTV
                     IGMLVMTVMLFNIVYNKFFDGAWFVVVLLFAYLGIFVVSEAIYAIWLEKLNVVPLELG
                     LWYPIFNDRKVDYKNIVLVSKFHPGVIHFLKNYVKSGHIPLVVHFRTDFDEDVPAQVP
                     AWYKNIDVPESTDTITAITRYVKRMKPQRVHLIPALVRGIDPISRYYFGNSMERLKYA
                     VSQFADLQVEYNKERIHIRVGDIVKRLFPRLGDRFFSDQKPTAEG"
BASE COUNT          659 a          563 c          627 g          654 t
ORIGIN      
        1 gtatcttgcg gaaatgatga taggcattct tcgattccat atgcgatgca ttgaaatccc
       61 tgtgtctcta tacagaaggt atggttctgt gcgaacggaa accacatcgg agcaatggca
      121 gtatttcttt tcagtcttgc gcttgatttt tggacgcagg ttcaccttgc tcggaaaact
      181 ctttcgcgtt tcttgaattc taaatttttg cttccataaa cgtgctttgt gtcgcagaat
      241 tggcgcgagg ccgtatgaaa aagattctgt acttcgttgt aattccgctc tttgtcatcc
      301 ttgcgctcgt ttcagccagt gcctggtatg ccctcaaagt agttttgaat gaaaacttcc
      361 tcgtgcagca aatagaatcg aacttaaacg ttcgagcgga agttcgtaag ctaaacgtga
      421 gcctgttttc cgccatgtca tctgtggaac tcgaaggagt cagtctcggg gcccgggact
      481 cttttgcaaa tcagggcaca cctctttcgg agaggcctcc tatgaaaggt tccgtcgtca
      541 cgattggcaa gattgatctg aaatttagcc ttctaccttt gcttcgcaga aagtttgaac
      601 tcaatcgatt catgctgagc agtccggttg tttctatgac gttattcgcc aatggtggta
      661 acagtctgaa gccattgttt caaaaaccgt tgattgtggg cagaaagcca aatccggcgc
      721 tggctgaaaa gccagcggaa acagcagcga aagaagagga gaaacccttc agcgcaaaag
      781 accttcctat atccgcggat ttgcgcgaga tcggactcaa agatgctact gtcaatttat
      841 tgatgcagaa aacggggcag ctcatccgaa tcagcggcct ggctctagta attgattcga
      901 tcgatattga tccggcagat ctggtcaaac acaacaatgc acatcttgat ttcgatgcca
      961 acgtaaatgt tatcagcgcc aaacagacgg acgctgcgag ttttctcatt cgctcgagtg
     1021 gcaatgtgac tccgtttgat tccaaaaccg gggaagtcag tacaggagta gagtacgccc
     1081 tcgtggttaa gaaagattct ttcatcactg gtatggcgat ttttgatcag ctcgccggtg
     1141 aacttcccgt tctcagtcag gtaggattct cctggaaaaa gctttctgaa aaagcagttc
     1201 ttcttcgcga cgtaaatgtt agagtgagat actctcacgc actcgttacg tttctggatg
     1261 acacagtgtt tccaaccgcg aactacgatc tactgctcaa gaagggaagc acaatcaatt
     1321 gcgcgacgaa cgaacacaat ttcaacgcgt ctgtgcgcgc cacggaagca gaatcaaaag
     1381 agatcctcgc gcgatttgac aggggcattg aaaagaaact caaagggcag agcgtagagg
     1441 acgtgcgaaa cagggttctt ggcaagttga tccagaacgg acaggtggat ctgccatttg
     1501 catccctggg aaatttcgcg aacccgagtg tgaagcttct ggcagaactt ccgagtttga
     1561 cggaagtact tacagggagc gtcaaagata ttttgaaaaa caaactggat cagaagattc
     1621 cgggcgttgg caaagagcta ctcaacaagc ttcccttcta ataagggaag cttgcaagcg
     1681 aagcgctcat agggtccgag gttgaaaggg ttcaaccttc ggctgtaggt ttttgatcgg
     1741 agaagaacct atcgccaagg cgcgggaaca atcttttgac aatgtcgccc acgcggatgt
     1801 gaattctctc tttattgtat tccacctgta agtctgcgaa ttgtgagact gcgtacttca
     1861 gacgctccat ggaatttcca aagtagtagc gactgatcgg atcaattcct cgcacgaggg
     1921 caggaatcag atgcacccgc tgtggcttca tgcgttttac gtatcgcgtg atggctgtga
     1981 ttgtatctgt gctttcgggt acgtctatgt tcttgtacca ggccggaacc tgtgctggca
     2041 catcttcgtc aaaatccgtg cgaaagtgaa ctacaagggg gatgtggccg cttttcacat
     2101 agtttttcaa gaagtgaatc acgccgggat ggaacttgga aactagaaca atgtttttat
     2161 agtctacctt gcgatcgttg aatatgggat accacagacc aagttcgagc ggcactacgt
     2221 tgagtttttc cagccagatg gcgtagatcg cttctgatac aacgaagatt ccgaggtatg
     2281 cgaacaacag tactaccacg aaccacgcgc cgtcaaaaaa cttgttgtac acaatgttga
     2341 agagcatgac tgtcatcacg agcatgccga tgacagtcat gccgttgatt ttgtgagcgc
     2401 gaatttgtct gagcaggtag gcgaagatcc caatgttcat ggttagaatc acagccagac
     2461 catacatgct ttcgatcgca tgactggatg ggaagaagta gac
//
LOCUS       DAOQJL010000564         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30397_length_2500_cov_3.997546, whole genome shotgun sequence.
ACCESSION   DAOQJL010000564 DAOQJL010000000
VERSION     DAOQJL010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30397_length_2500_cov_3.997546"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLG78_17980"
     CDS             complement(<1..432)
                     /locus_tag="PLG78_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25068.1"
                     /translation="MKTWLVILFLFVQALSADTKSEKSDKQPREPAKTETAKTEPAKT
                     EPAKTEPAKAEPAKTDKPENKADRPERPSEKPPHAVCIEVTRRIPSAAMPYNGPSVKT
                     IRATGIQIGKRIVTDLENVRGAISLTVRQPDQDTATQGRLLF"
     gene            complement(429..1562)
                     /locus_tag="PLG78_17985"
     CDS             complement(429..1562)
                     /locus_tag="PLG78_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25069.1"
                     /translation="MKTLYAIILLLALSSLSAREDMPFIPGPADLKKQTRPQTTPPNV
                     QPRDQKLQPQTGPGKPNDQTSIRDGKPAEPVPMKGDRPDLPSPGNTIRFEQAQTPRDF
                     PYQEYSRLMTDSIRLAAERSKMEPEIALHREQEIREELRLLFPRGLYRFGSTCPLKKL
                     FSSSAIEFRITCLPDDVQNKIRFARGRTDAIRNAFEFLSEGRTITANLQLLETGVGSD
                     GLEFIFREKSFQYENRFIEALRDESHMTEQDLLQPEWVSRKLPGIISQSYTESDLAAA
                     REVFPRSAWFPFHKDCPLKIQASTLLDGESTTRIELAPTCMPGRTAVVRVSEQEMNDE
                     SALRSDYGTALFAEMRLSGIMLVDGRLHLDWDGVRNVRRAEGK"
     gene            complement(1559..2458)
                     /locus_tag="PLG78_17990"
     CDS             complement(1559..2458)
                     /locus_tag="PLG78_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25070.1"
                     /translation="MIADTEEENSLDTEEEEEQVAAEIQPEEAAGEPDFDEEEHRLTL
                     RQKIYIGIGLTVSFFFFTLLLFPKDLFLRAILARTAASVRIDFSSADPGLFGQTFQTL
                     SVILPDGTAILVDSLDSSLHAFDLMRGSAEGKVQLGKSDVSTTGLALGWASGDITTNL
                     SGYSEGLTGMRGDLKIGLRDVNFVRFPPAAANLVPIRPDKIKVSSLELPLSFRDGGLV
                     LQNGLIASNLFIVKINTKGSYQGPSLDGMNLDGQICLKPDRELEQKEPDVFGMYVFAG
                     GAAGGELCFQVRGTVGRPQFNKL"
BASE COUNT          542 a          627 c          659 g          672 t
ORIGIN      
        1 gaagagcagc cttccctgtg tcgccgtatc ctggtccggc tggcggactg tgagagaaat
       61 ggctcctcgc acattctcta gatctgttac aatccttttt ccgatctgaa ttccggtggc
      121 gcgaatcgtt ttcacggaag gaccgttata cggcattgcg gctgatggta ttctgcgtgt
      181 gacttctatg caaactgcgt gaggaggttt ctccgacggt ctttccggcc tatctgcttt
      241 attctctggc ttatccgttt tcgcaggttc cgcttttgca ggttctgtct ttgcaggctc
      301 ggttttagcg ggttctgttt ttgcggtttc cgttttcgcg ggctcgcgcg gttgcttatc
      361 agacttctct gattttgtat ccgcgctcag agcctgtaca aaaaggaata gaattaccaa
      421 ccaggttttc atttgccttc tgctctcctg acgttgcgaa ctccgtccca gtccagatgc
      481 aatcgtccat ctaccagcat gattccagaa agcctcatct cggcgaaaag tgccgttcca
      541 tagtcgctgc gaagcgcgga ttcatcgttc atttcctgtt cgctgactcg cacgaccgcg
      601 gtgcgtccag gcatacacgt gggtgcaagt tcgatcctcg tcgtcgattc gccatcgaga
      661 agagtggatg cctggatctt aagaggacag tctttgtgaa atggaaacca ggctgaacgc
      721 gggaacactt cacgcgcggc tgcgagatcg gattcagtat aggattgaga tatgattccg
      781 ggcaactttc ttgagaccca ttcgggttga agcagatcct gctccgtcat atgagattcg
      841 tcgcgcagag cttcgatgaa gcgattttcg tattgaaaag atttttcgcg gaatataaac
      901 tccagaccgt ccgagcccac cccggtttcg agaagctgaa gattggctgt gatggtgcgg
      961 ccttccgaaa gaaattcgaa agcgtttctg atagcgtctg ttcttccgcg cgcaaagcga
     1021 atcttgttct gcacatcatc gggcagacat gtaattctaa attcgatcgc actggatgaa
     1081 aaaagcttct tgagaggaca agtagaacca aaacgataaa gacccctggg aaacagaaga
     1141 cgcaactctt ctcggatttc ctgttctcga tggagtgcaa tttctggctc cattttgctg
     1201 cgttctgctg ccagacgaat gctgtctgtc atcaagcgag aatattcctg atatgggaag
     1261 tcacgaggtg tttgtgcctg ctcaaagcga attgtgtttc cgggagacgg tagatccggg
     1321 cgatcgcctt tcataggtac aggttctgcc ggtttaccat ctcgtatgga cgtctgatca
     1381 ttgggcttgc caggaccagt ttgaggttgc agcttctgat cccgtggttg aacgttaggc
     1441 ggcgttgtct gagggcgtgt ctgctttttc agatcggcgg gtcccggaat gaaaggcatg
     1501 tcttctctgg cggaaaggga ggacagtgca agaagcagga tgattgcgta gagagtcttc
     1561 atagtttgtt gaattgcggt cttccgaccg ttccgcggac ctggaagcac agttccccac
     1621 cggccgcgcc tccagcaaac acatacatcc cgaatacgtc cggttctttt tgttccagct
     1681 cccgatccgg tttcagacaa atctggccgt ccaggttcat accgtctaga gatggcccct
     1741 gataggagcc tttcgtgttg attttcacaa taaacaaatt gcttgcgatc aggccgtttt
     1801 gaagcaccag tcctccgtcc ctgaatgaga gtggcagctc cagagagctc actttgattt
     1861 tgtccgggcg aatgggaaca aggttcgcgg ctgccggtgg gaatcgcacg aagttgacgt
     1921 cgcgtaatcc aatcttcaga tccccgcgca tcccggtcag cccctccgag tagccggaga
     1981 gattggtggt gatatctccg ctggcccatc caagtgcaag acctgttgtg gaaacatctg
     2041 atttgcctaa ctggactttc ccttccgcgg aaccgcgcat tagatcaaat gcatgaaggc
     2101 tggagtcgag gctatccacg agtatggccg ttccatctgg aagaatgacg cttaacgtct
     2161 gaaacgtttg gccaaacaat ccgggatctg cggatgagaa atcaattcgt acggatgctg
     2221 ccgtgcgcgc gagaatggcc cgaagaaaca aatctttggg gaaaaggagt aatgtgaaaa
     2281 agaaaaagga aactgtcagg ccgattccaa tgtaaatctt ctggcgaagg gtaagtctat
     2341 gctcctcttc gtcgaagtca ggttctccag cggcttcttc aggctgaatc tccgccgcaa
     2401 cctgctcttc ttcttcctct gtatcgagcg aattctcttc ttctgtgtcc gcgatcattt
     2461 ctttggcctc gttatcacga gagtgatgcg catatcatag
//
LOCUS       DAOQJL010000565         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30484_length_2495_cov_4.149590, whole genome shotgun sequence.
ACCESSION   DAOQJL010000565 DAOQJL010000000
VERSION     DAOQJL010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30484_length_2495_cov_4.149590"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLG78_17995"
     CDS             complement(<1..368)
                     /locus_tag="PLG78_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25071.1"
                     /translation="METKSFLNLLLFNAGYASVCSSPPDRIVSASNWKNVQTMLQYEM
                     FKGSNAPASVTTFGSPGAATSKWTGGVLAANGKIYGMPRSAGTVLEIDPDTRTLLQFG
                     SIAGLNQYTGGVLAQNGKIYG"
     gene            complement(437..1003)
                     /locus_tag="PLG78_18000"
     CDS             complement(437..1003)
                     /locus_tag="PLG78_18000"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HNE25072.1"
                     /translation="MNFFGKIKGRFLALTLAACILGGSIGCHGGFVLTSKWNGLITSI
                     GNKWIRWIVFLVLSFVYGFTILADAIVFNSVEFWTGSNPVALNYNEKGEYTRTLTSGS
                     ETAVMTYRNFGETLDVALFKNGVQTDSLRLIKSEPGVLYRQSNGQFEKIIVSEETRGN
                     LRIIRAGEKSAAVPAAVYQVELARSGSM"
     gene            complement(1000..1683)
                     /locus_tag="PLG78_18005"
     CDS             complement(1000..1683)
                     /locus_tag="PLG78_18005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE25073.1"
                     /translation="MKLTLRLLSPALLFALAILIVIVYQVIRVGDKPGDAAFAIQESV
                     PLMILLAQLLWLLLLLYLKWRDSLTWTDLGWKKPQKSDSLIAIVSGLGLAAAYVRLLS
                     PAHTWLQRSIGDYVPAGGLISSLGSMKAVFFVANVLFAPFVEETIFRGYALPRLKERG
                     EFGVLWSCLFFGLIHWAGGFWYMILTGAVAGGLFAFIYLRNHSTTAAWLCHLSLNAAE
                     FAYVAWRDL"
     gene            complement(1717..2178)
                     /locus_tag="PLG78_18010"
     CDS             complement(1717..2178)
                     /locus_tag="PLG78_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE25074.1"
                     /translation="MRITQVMIRDLACLEGNATVADAARLMKEKDIGAVLITDSGKLV
                     GIVTERDLALKVLAEGRGGDVPITEIYTPNPETAREGASVAECIVLLRDAHFRHLPIV
                     DLEKKPVGIVSTRDFYKKLAEGMDRIFEETGFSLQIAEGEDPYTVILGPSV"
     gene            complement(2175..>2495)
                     /locus_tag="PLG78_18015"
     CDS             complement(2175..>2495)
                     /locus_tag="PLG78_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25075.1"
                     /translation="KGVKVIVKDTFNEKEIEIAMRVKSAIIGINHRDLDTLKMDLSLT
                     PRLAPVVRRELPGCVIVAESGVESRDGLKTVDEFVDSVLIGTAFMKSQNVASIWQEIF
                     GGAR"
BASE COUNT          624 a          659 c          603 g          609 t
ORIGIN      
        1 ccatagatct ttccgttttg agcgagtacc cctccggtat actggttgag gccggcaatg
       61 ctcccaaact gcagcaaggt gcgggtgtcc ggatcgattt caagaacggt gcccgcgctg
      121 cgcggcattc cgtaaatttt tccgtttgcc gcgagtacgc cgccagtcca tttcgaagtc
      181 gcagctcccg ggctgccaaa cgtagtgaca gacgccggag cattcgatcc cttaaacatt
      241 tcgtactgca acatggtctg gacgttcttc cagttcgaag cggagacaat tcgatccggt
      301 ggcgaagagc agacgctcgc atagccagcg ttaaatagga ggaggttgag aaagcttttg
      361 gtttccacgc cattcgccgg ctgctggcaa cttgccagca ccagaaggca aacagaaatc
      421 gatttattcc tctgcatcac atggagccag agcgcgccaa ttccacctga taaactgccg
      481 caggaaccgc agcagacttt tcgcctgcac gaatgattcg cagatttcct ctggtttctt
      541 cgctaacgat tattttttca aactgaccgt tggactggcg ataaagaaca cctggttcag
      601 atttgatgag cctcagagaa tccgtttgca caccattctt gaaaagagca acgtccagag
      661 tttcgccgaa gttcctatag gtcatcactg cagtctctga tccagatgta agggtgcgag
      721 tgtattcgcc tttctcgttg tagttgagtg ccactggatt ggatccagtc cagaattcta
      781 cggagttaaa tacgatggcg tctgcgagga tcgtgaaccc atagacgaat gatagcacca
      841 gaaatacgat ccatcggatc catttgtttc cgatgcttgt aatcagcccg ttccatttag
      901 aagtgagaac aaaaccgccg tggcaaccaa tgcttccgcc taaaatgcag gcggccagag
      961 tgagtgcgag aaagcgtccc tttatcttgc caaagaaatt catagatccc tccaggcgac
     1021 gtatgcaaat tccgccgcgt tgagcgatag gtgacagagc caggccgcag tcgtcgagtg
     1081 atttcgtaaa tagatgaacg caaacaatcc acctgccaca gcacccgtga gaatcatgta
     1141 ccagaatcca cctgcccaat gaatcagccc aaaaaagagg caactccaga ggacgccgaa
     1201 ctcaccgcgt tctttcagtc tcgggagagc gtatccccgg aagattgttt cttctacaaa
     1261 gggggcaaac aggacattgg ccacgaagaa cactgctttc atggatccaa gggaggagat
     1321 gagaccacct gccggaacat agtcaccgat cgacctttgc agccaggtat gcgccggaga
     1381 taaaagccgt acgtaagctg ccgcaaggcc caggccggac acaatcgcga tcagagagtc
     1441 cgatttttgt ggcttcttcc atccgagatc tgtccaggta agggagtctc gccatttgag
     1501 gtagagcaac agcagaagcc acaaaagttg tgccagaaga atcatcagag gaacggattc
     1561 ctggattgcg aatgcagcgt ctcctggttt gtcccccact cttattactt gataaactat
     1621 aactattaag attgccagcg caaacaaaag tgcaggcgaa agaagacgta gagtgagttt
     1681 catgctatgg ccgtgtatac gtgattcttg atttgctcag acagaaggac caagaatcac
     1741 ggtgtaagga tcttcccctt ccgcaatttg cagactgaat ccggtctctt cgaagatgcg
     1801 atccattcct tccgcaagct tcttgtaaaa gtctcgagtg gaaacaattc ccaccggctt
     1861 tttttcaaga tccacgatag gcaaatggcg aaagtgagcg tctctgagaa gcacaatgca
     1921 ttccgcaaca gaggcgcctt cgcgggctgt ttcaggattg ggagtataga tttccgtaat
     1981 gggcacatct ccacctcgac cttctgcgag caccttgagc gccaggtctc tttccgttac
     2041 aatgccgact aactttccag agtcagtgat cagcaccgcg ccgatgtcct tttccttcat
     2101 gagcctggct gcgtctgcca cagtagcgtt cccttccagg cacgccagat cccggatcat
     2161 tacttgcgtg attctcatct ggctcctccg aagatttcct gccatataga ggccacattc
     2221 tgtgatttca taaatgctgt gccgatcagc accgagtcca caaactcatc tacggttttg
     2281 agtccgtctc tggattccac gcctgattcg gccacaatga cacatccggg aagctcgcgt
     2341 ctcactacag gtgcgaggcg cggtgtcagg ctaagatcca tttttagagt gtccaggtct
     2401 ctgtgattga ttccaatgat cgcggatttt actctcatcg cgatttcgat ttctttttca
     2461 ttgaaagtat cctttacaat tactttaacg ccttt
//
LOCUS       DAOQJL010000566         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30511_length_2494_cov_4.026650, whole genome shotgun sequence.
ACCESSION   DAOQJL010000566 DAOQJL010000000
VERSION     DAOQJL010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30511_length_2494_cov_4.026650"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLG78_18020"
     CDS             complement(<1..222)
                     /locus_tag="PLG78_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25076.1"
                     /translation="MNAYLISQLPLISGIFALLILAPALVRGKDLISAIISILSVAGT
                     IVLMCLLPASSKYYGGAILVTALGKSVAGI"
     gene            complement(219..1664)
                     /locus_tag="PLG78_18025"
     CDS             complement(219..1664)
                     /locus_tag="PLG78_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE25077.1"
                     /translation="MELLTIIAFPVLAFVVHSFLPKQRGAFLVSTLFYSALLVVQSLR
                     AFISPVYLNAGGVRLWGDFALVPSFYLTGLNAPLLLLIGLVLPFVALLLRSVHAEDKR
                     YTLSAWVFLLGLAGTFISQSLILFYVFFETSLIGAYFWIGLFGRSSVSGSPGGWSGLT
                     RFFLFTLVGSLSMLASIAALSSGGKDVRFTDVPIVLGTFSDSGRFWIFAGFALAFAIK
                     LPLFGFHGWLRETYAMAPPAARALLSGIMSKLGAYGFLLLAVYFGIEFRLHSKLIIAL
                     CAAGVLYGGLVSLSQKRFLDVVVYSSLAHLNLIVMGLAAMSGSVSDTTIVSACVLQMF
                     NHGLIMAAILVYDARMGSPDESQSGMREHAGILSALLLLSLFAAVSLPGLSSFPPEFA
                     ILYAAFKTSIWIALVAGLGLLISAAALIRSYHRIYAGNFAGSANVASSGEVSRMEAVI
                     GVIFGLVWIGLGILPGLMLSPVAAALGVIKL"
     gene            complement(1669..>2494)
                     /locus_tag="PLG78_18030"
     CDS             complement(1669..>2494)
                     /locus_tag="PLG78_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25078.1"
                     /translation="ILAAHHEQDLHKLRGSLKHLPVTRVALWTGAIGGAGLVPFLASG
                     FFSKEAVLHSISEASFGVLGLTISGGMFYWFVFGAELLGVVYLFRMLALLESKPESHE
                     SHASHAIEEKGVFIRAVLAILVLVAIAFGVASSPFVSGFSHFLFDKAEHVSIVDICLA
                     SGSSILVAIIVFVVFRADAAEAAVGTGARSGLIRNLFYFDKIYSAVLLAPLGAIGSAT
                     ARFIEVPIFQGALRGGAAVSRTVSSAFSRMQKGLISQYAVIMIVSAAIVMLLALLA"
BASE COUNT          808 a          667 c          606 g          413 t
ORIGIN      
        1 aatacccgcc acggattttc caagcgcagt gacaagaatc gcgccaccgt agtattttga
       61 agacgcaggc aacagacaca tcagtacaat tgtcccggcc acggacagga tagagataat
      121 agcggaaatc aagtctttgc cgcgaacgag cgccggcgcc agtatgagca aagcaaagat
      181 accagaaatc agtggaagct gcgaaatcaa atatgcattc ataacttgat cactccaagt
      241 gccgccgcca caggcgagag catcagccct ggaagaatcc ccagaccaat ccagaccagt
      301 ccgaagatga caccgatgac agcctccatc ctggaaactt ctccactcga tgccacattc
      361 gcgcttcctg caaaattacc tgcgtaaatt ctgtgatagc tgcgaatgag agccgcggca
      421 gaaatcaaaa ggccgaggcc tgcgaccaga gcaatccata tagaagtctt gaacgcggca
      481 tacagaatag caaactcggg gggaaagctt gacagacccg gcaaacttac cgcggcaaac
      541 aaagaaagaa gcaataacgc agacagaatc ccggcatgct ccctcattcc tgactgactt
      601 tcatccgggc ttcccatgcg cgcatcgtat acaaggatcg cggccatgat caatccatga
      661 ttgaacatct gcaacacgca ggcagatacg atggtcgtat ctgaaacaga accactcatc
      721 gcagcaagcc ccatcacgat caagttcagg tgcgcaagag aagaatatac aacaacatcc
      781 agaaaacgtt tctgcgatag actcaccagt ccaccgtaca aaactccagc ggcacacaat
      841 gcgatgatca acttcgaatg cagtcgaaac tctatcccaa agtaaactgc gagaaggaga
      901 aaaccgtacg caccgagctt ggacatgatg ccggacaaga gcgctcgcgc agctggcggc
      961 gccattgcgt aggtttcgcg cagccatcca tgaaagccga ataacggtag cttgatggcg
     1021 aacgcaagag cgaatccagc aaaaatccag aagcgacctg aatcagagaa tgtaccgagc
     1081 acaatcggca catctgtaaa acgcacgtcc tttccgcctg aggaaagagc tgcaatcgat
     1141 gcgagcatgg agagactacc caccagagtg aatagaaaga atcgagtcag accagaccaa
     1201 ccgccaggag aaccggacac actggatcta ccaaaaagcc cgatccagaa atacgcgccg
     1261 atcagagaag tttcaaagaa cacataaaac agaatcagag attgagagat gaatgtccca
     1321 gcaagaccca gcagaaatac ccaggcagaa agagtgtagc gcttgtcctc cgcgtgaacc
     1381 gaacgaagca gaagggcgac gaaaggaagg accaggccaa tcaacaaaag aagaggggcg
     1441 ttgagtcctg tcagatagaa agacggaaca agtgcaaaat ccccccagag gcgtactccc
     1501 cctgcattga gatagacagg agaaatgaac gcgcgaaggg actgcaccac gagaagggcg
     1561 gaatagaaca aagtggaaac cagaaacgct cctctctgtt ttggaagaaa agagtgcacc
     1621 acaaaagcca ggaccggaaa agcgatgatc gtaagtaatt ccatagtttt atgccaacaa
     1681 tgcaagcagc atgacaatcg ccgcagatac aatcatgatc accgcatact ggcttatcag
     1741 ccctttctgc atgcgggaaa atgctgaaga aacagtccga ctcacggcgg cgccaccgcg
     1801 aagtgcacct tgaaaaatcg gcacttcaat gaagcgagcc gtggcagatc ctattgctcc
     1861 cagaggcgcg agtagaactg cagagtaaat cttatcaaaa tagaacaaat tcctgatgag
     1921 cccgcttcgc gcgcctgtgc ccactgcagc ttcggctgcg tctgcacgaa acactacaaa
     1981 tacgatgatc gcaaccagga tggaacttcc agatgccagg caaatgtcta cgatcgatac
     2041 atgctctgcc ttatcaaaaa ggaagtgcga aaacccactc acaaaaggag aagatgcaac
     2101 gccaaatgca atcgcgacaa gaaccaggat ggcgagaact gcgcgaatga agactccctt
     2161 ctcctctatt gcgtgtgatg catgcgattc gtgggattct ggtttgcttt caagcagcgc
     2221 aagcatgcga aacaaataca cgacgcccag aagttctgcg ccaaaaacaa accagtagaa
     2281 catgccgccg gagatggtga gtcctaagac cccgaaactt gcttctgaaa tcgaatgcag
     2341 cacagcctct ttggagaaaa aaccagaggc cagaaatgga accagccccg caccgccgat
     2401 cgctccagtc cagagcgcta cacgagtaac gggaagatgc ttcagagatc cgcgcagttt
     2461 atgcagatcc tgttcatgat gtgccgcgag aatg
//
LOCUS       DAOQJL010000567         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30642_length_2488_cov_4.725442, whole genome shotgun sequence.
ACCESSION   DAOQJL010000567 DAOQJL010000000
VERSION     DAOQJL010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30642_length_2488_cov_4.725442"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLG78_18035"
     CDS             complement(<1..937)
                     /locus_tag="PLG78_18035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE25079.1"
                     /translation="MRFTTLAFLVFFIAVYVVYWTVTGRTRLIFIFLASILFYATWSV
                     GFAIHFVSMVGFTYLIVKWMQKSSSSVAFYVGVIANLGNLFLFKYFYLLLEILKDATG
                     SNIFKATEFNSWLFSRTGYDSIVLPLAISFYTFVLVAYLFDVHRGIIKEKHSFLEFGV
                     FAMYFPHLVAGPIIRHSDFFYQLGDIKADQDKIVRGMFLILTGLVKKVAIADNILPLF
                     SRVFLKPDEYSGAANAMAAVSFSIWVFCDFSGYTDLARGMSLLLGIELPENFRAPFLS
                     RSVRELWRNWHSTLGTWMRDYVYIPLGGSRRGEVRS"
     gene            complement(939..2066)
                     /locus_tag="PLG78_18040"
     CDS             complement(939..2066)
                     /locus_tag="PLG78_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE25080.1"
                     /translation="MNILRDTRIAIPLGFLLGLELFLQTGLYKSLMQPRSYADNVNRI
                     LRMAGQDKDPQALILGTSVAYQGIQVPELNELLKKDAITVESGACEGAKLETQHLIMR
                     ALLDRMPHVKTVIHVVEASHPGTARFHQDQANRSMAAQFPRMEALHVLKSHNLKLEPS
                     DYVYFFVRSITYQKDMRDFVLDPLDRFKGIGRRFREKPTGYIYENKYEYKLSAFPAKN
                     PVECVEVATRANIEQMGKEVTDQHHRNAVVRTCQIVLMDPLAGSGNAQWTALFFQRLK
                     VMYKDITDRGIKIIVVFSPYSDVIKDLNAEYRIGEWQNGLAAITARENYTTVDLRKSL
                     DGPRNGDYYYDTIHLNREGATVFTKALADAIRPILLKNTAR"
     gene            complement(2083..>2488)
                     /locus_tag="PLG78_18045"
     CDS             complement(2083..>2488)
                     /locus_tag="PLG78_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25081.1"
                     /translation="YNIFARLFTLWALSFGFVWFRAEQMHVGNLILGRMYGIADIHAA
                     WAGLKVWLAGGGSHALSAATGKIPEHSVLSSYWILLGLYFAYEYFFYWYRLSTFRLPE
                     NRKKWIALMIFMLFCIVTLSAPESANFLYFQF"
BASE COUNT          640 a          585 c          653 g          610 t
ORIGIN      
        1 gagaacgaac ttctcctctt ctagagccgc ctaacggtat gtaaacgtaa tcgcgcatcc
       61 acgtcccgag agtggaatgc cagtttctcc agagttctcg cacgctgcgt gataagaatg
      121 gtgctcgaaa attttccgga agttcaatcc cgaggagtag cgacatgccg cgcgctagat
      181 ccgtatagcc ggagaaatcg cagaagaccc agatagaaaa gctaaccgcg gccattgcgt
      241 ttgccgctcc agagtattcg tctggtttga ggaagacgcg agagaacaag ggaagtatgt
      301 tgtccgcaat cgccactttt tttaccagac ccgtgagaat cagaaacatc ccgcgaacaa
      361 ttttatcctg atctgctttg atgtctccca gttgatagaa gaagtctgaa tggcgaatga
      421 tcggcccagc cacaaggtgc gggaaataca tggcaaacac accaaattcc aggaagctat
      481 gtttttcttt gatgatcccg cgatgaacgt caaagagata ggccaccagg acaaacgtat
      541 agaagctaat ggcaagaggc agaacaatgc tgtcatatcc cgtgcgactg aagagccacg
      601 aattgaactc ggtcgctttg aaaatgttag accccgttgc gtctttgaga atttccagca
      661 gcaggtagaa atacttgaat agaaacaggt tcccaaggtt tgcaattaca cccacataga
      721 acgcgacgga agaagaggac ttctgcatcc actttacgat cagataagtg aagcccacca
      781 tagacacgaa gtgaattgca aatccaacgg accacgttgc gtaaaagaga atggatgcga
      841 ggaatatgaa gatgagtctt gtgcgaccgg ttacagtcca gtagacaacg tagactgcga
      901 tgaaaaaaac caggaatgcg agagtagtga agcgcatttt atctggccgt gttcttcaag
      961 agaatggggc gaatcgcgtc tgcgagtgct ttcgtgaaca cggttgcccc ttcgcgattg
     1021 aggtggattg tgtcatagta gtagtcccca ttccttggcc cgtccagtga ctttcgcaga
     1081 tctacagtag tgtagttttc gcgtgcagtg attgcggcga gaccattttg ccattcgcct
     1141 attctgtatt cagcgttcag gtctttgatt acatcagagt aaggggagaa gaccacgatg
     1201 atcttaattc cgcgatccgt gatgtctttg tacattactt tcaaacgctg gaagaagagt
     1261 gcggtccatt gagcattgcc cgaaccggcc agtggatcca tcagcacaat ctggcatgtt
     1321 cgcaccactg cattgcgatg atgctgatcg gtaacttctt tgcccatttg ttctatgttt
     1381 gctctggtgg cgacctcgac gcattcgacg ggattctttg cgggaaaggc ggagagtttg
     1441 tactcatatt tgttttcgta aatgtatccg gttggttttt cgcgaaatcg tcttccgatt
     1501 cctttgaagc gatcgagcgg atcgagtacg aaatcgcgca tatccttctg gtaggtgatg
     1561 gagcgcacaa agaaatacac gtaatcggaa ggttcaagct tcaggttgtg gctttttaaa
     1621 acatgcagag cttccatgcg ggggaattgc gccgccatgc ttctgtttgc ctgatcctgg
     1681 tgaaagcgag ccgtgcctgg atggcttgcc tctacaacgt ggatcactgt ctttacgtga
     1741 ggcatccggt ccagaagtgc gcgcataatg agatgctgtg tttcgagctt tgcgccttcg
     1801 catgcgccgc tttccactgt gatggcgtct ttcttgagaa gttcattgag ttccggtacc
     1861 tggattccct gataggcgac ggacgttcca agaatgagcg cctgcgggtc tttgtcctgg
     1921 ccggccatgc gaagaattcg attcacgttg tccgcgtaag agcgcggttg catcagcgac
     1981 ttatagagtc ctgtttgcag aaagagttcg agacccagca agaaacccag ggggatcgca
     2041 atgcgcgtgt cgcgcaggat attcatcagg tttttcatat atttaaaatt ggaagtacag
     2101 gaagttagcg ctctccggcg cgctcagtgt aacaatgcag aacagcatga agatcatcag
     2161 ggcgatccat ttctttctgt tttccggaag acggaacgtg gacagtctat accagtagaa
     2221 gaaatactcg taagcgaagt agagacccag tagaatccag taagaagaaa gaacgctgtg
     2281 ctccggaatt tttccggtcg cagcgctgag cgcgtgcgag ccgccacccg ctagccacac
     2341 tttaagtcca gcccaggcag cgtggatatc cgcgattcca tacatgcgtc ccaggatgag
     2401 attcccaacg tgcatctgct ccgctctaaa ccagacgaat ccaaacgaga gcgcccatag
     2461 agtgaagagt cttgcgaaga tgttgtaa
//
LOCUS       DAOQJL010000568         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30643_length_2488_cov_4.591451, whole genome shotgun sequence.
ACCESSION   DAOQJL010000568 DAOQJL010000000
VERSION     DAOQJL010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30643_length_2488_cov_4.591451"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLG78_18050"
     CDS             complement(<1..382)
                     /locus_tag="PLG78_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNE25082.1"
                     /translation="MHGALPGEKVRVRLTKRNRNHSFAVLESVIEPSPSRIESDCSIF
                     PECGGCSFRNISYEEELSVKTRLVREFRPLGEACGDFEVFSAAPDAYRTHVRIQFDGK
                     QAGFFSLHSNRIVPLPSTGCANLPK"
     gene            complement(447..1370)
                     /locus_tag="PLG78_18055"
     CDS             complement(447..1370)
                     /locus_tag="PLG78_18055"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNE25083.1"
                     /translation="MRFLLILAALLMASSCRTPPGCSSTFCDYKDELTVYGRNVHLLY
                     EDNFREISSNCAFRTELTTGDYVPMTQQQALTILRNMAGEMGGNALVFQSFRKSKDAS
                     GRVYVCSNFGSATSGQVQSNQISTQSTTQTQSQVSSVVIQPDTRVTSSINTRQNTTPT
                     NTQQQNVNLNQVQPIQPVSQAMTVNDTGRKLSWQTCSMGQSYSGGKCSGQAVAETFQS
                     ASSYCSSQSTAGYTYRLPDRIELASLLTSTGTPKADPRVFPDTKPDIYWTRESYNNSP
                     ITNWVVDFGAGGSFGYGTENQAYVRCVANLP"
     gene            complement(1371..1649)
                     /gene="xseB"
                     /locus_tag="PLG78_18060"
     CDS             complement(1371..1649)
                     /gene="xseB"
                     /locus_tag="PLG78_18060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNE25084.1"
                     /translation="MAQKKKPTFEEALSELEEITRLLESGELTLDQSVEAYEKGMELK
                     KACYEMLSTAEKKLEYLEKKDGELKRVALASENMRADNRLFEDDDSED"
     gene            complement(1657..>2488)
                     /locus_tag="PLG78_18065"
     CDS             complement(1657..>2488)
                     /locus_tag="PLG78_18065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25085.1"
                     /translation="NGALAKGISFVRARPGSLFSRLDSIGESIKNDLPVYLARSGDSV
                     KAPDHAQVIGPSDALHIHATATIHPGVIFNTEDGPIVVDAGAKISPFSYLSGPLYVGP
                     RAQLDHLKIANSIVGEGCRLGGEIESSIFGDFTNKHHEGFVGHSLIGNWVNLGALTTT
                     SDLKNNYGKIRLHLPGAVVETGRIKFGSILGDCVKTAIGTMLNTGSVVDAGSSVFGGR
                     PPAYLGPLSWGLTGQTYELDRFLKDCETIFARRSQTVPAAMRRMVELSVSLDAGYTGS
                     "
BASE COUNT          604 a          635 c          627 g          622 t
ORIGIN      
        1 ctttaggaag attcgcgcaa ccagtagaag gaagaggcac aattcgattt gaatgaagag
       61 aaaagaaacc agcctgtttg ccgtcgaact gaatgcgaac atgggtgcga tatgcgtctg
      121 gagcggcgga aaagacttcg aagtcgccac acgcttctcc cagtgggcga aattcacgaa
      181 caagccttgt ctttacggat agttcttctt cgtatgagat gtttctaaaa gagcagccac
      241 cgcattcagg aaaaatgctg caatcagatt caatgcgtga aggagaaggt tcgattacgc
      301 tttccaggac tgcgaacgaa tgatttctat tacgctttgt caggcgaacg cgcactttct
      361 ctcctgggag agctccgtgc acaaatacag gatgctcccc gggagccagc gtaaatccgt
      421 tctgtaccca cttcagcggg cgaagttcaa ggaaggttgg caacgcagcg aacgtaggct
      481 tgattttctg tgccgtatcc gaaagatcca ccggcgccaa aatcaacgac ccagtttgtg
      541 atggggctgt tgttgtaaga ctctctggtc cagtaaatgt ccggcttggt gtccgggaaa
      601 actcttggat ccgccttcgg cgtcccggta gaagtcagaa gagaagctag ttcaatgcga
      661 tcaggcaggc gatacgtata gccggccgtg ctctgcgaac tgcaatacga ggaagcggat
      721 tgaaatgttt ccgccactgc ctgccccgag cattttccgc cagagtaact ctgacccatg
      781 ctgcaggtct gccagctgag ctttcttcct gtatcattca cagtcattgc ctgggagact
      841 ggctggatgg gctgaacctg gtttagattg acgttctgct gttgggtgtt ggtcggagtt
      901 gtgttctgtc ttgtattgat gctggatgta acacgcgtat caggctggat tacaacagag
      961 ctgacctgag actgagtttg cgttgtgctt tgagtggaga tctgattgga ctgcacctgg
     1021 cccgaagtgg cgcttccgaa attggaacag acgtagactc gaccggacgc atccttactt
     1081 tttctaaatg attgaaaaac aagagcattt ccgcccatct ctcccgccat gtttcgcagg
     1141 atggtcaggg cctgttgctg ggtcataggc acatagtcac ctgtcgtgag ttccgtgcga
     1201 aatgcacagt tagacgaaat ctcgcggaag ttatcttcat atagaagatg aacgttgcgt
     1261 ccgtagacgg tcagttcgtc cttgtaatcg caaaaagtgc tgctgcagcc gggcggagtt
     1321 ctgcacgaac tggccatcaa aagcgccgcg aggattagta agaatcgcat tcagtcctcc
     1381 gaatcgtcgt cttcaaaaag acgattatcc gctctcatat tttcggacgc cagagctaca
     1441 cgcttcaact ccccatcctt cttttcaagg tattcgagct ttttctcagc cgtagaaagc
     1501 atctcatagc aggccttttt cagttccatg cccttctcat atgcttccac agactggtcg
     1561 agcgtcaact ccccactctc cagtagacga gtgatctcct ctagctccga cagtgcttct
     1621 tcaaacgtag gctttttctt ttgagccatg ttggattcag cttccggtgt agccggcgtc
     1681 aagtgaaacg gacagctcta ccatacggcg catggcggct ggcacagtct gagagcggcg
     1741 agcaaaaatt gtttcgcaat ccttgaggaa gcgatccagt tcatatgtct gaccggtaag
     1801 tccccaggaa agtggcccga gatacgccgg aggcctcccc ccgaacacgc tcgatcctgc
     1861 atccacaact gatcctgtgt ttagcattgt gccaatcgca gtcttgacgc aatcgcccag
     1921 aatggagcca aatttgattc gccctgtttc caccactgct ccaggcagat gcaatctgat
     1981 cttgccgtaa ttgtttttca aatcggatgt ggtggtgagc gcacccagat tcacccagtt
     2041 gccaatcaaa ctgtgaccca caaaaccctc gtggtgtttg tttgtaaaat caccaaagat
     2101 agacgattct atttctccgc caagcctgca accttcgccc acgatcgaat ttgcaatctt
     2161 caaatgatcc aattgcgcac gcggcccaac atacaatggg cctgaaagat agctgaatgg
     2221 agagatcttc gctcctgcat ccacaacgat gggaccatcc tctgtgttga aaattacacc
     2281 gggatgaatt gtagcagttg cgtgaatatg gagcgcgtcc gacggtccga tcacctgtgc
     2341 atggtcaggt gcttttacgg aatcgccact gcgtgccaga tatacaggca ggtcattctt
     2401 tatggattcg cctatggaat ccaaacgcga aaaaagagaa ccaggtcgag cgcgaacaaa
     2461 agagattcct tttgcaagtg caccgttt
//
LOCUS       DAOQJL010000569         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30875_length_2478_cov_4.513413, whole genome shotgun sequence.
ACCESSION   DAOQJL010000569 DAOQJL010000000
VERSION     DAOQJL010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30875_length_2478_cov_4.513413"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLG78_18070"
     CDS             <1..457
                     /locus_tag="PLG78_18070"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE25086.1"
                     /translation="CETPAALLQNLNAALVSGSAGSPVTAQYLIVAPDRRSATLASAG
                     HPPPILSRNGETIQMLSSGPMLGVVDPIQARFFRTELQAGDRLFLYTDGLVPTKQDAP
                     DLRMHPLHKAIINSGNTDLESQLKEIVRSLRAGQAGFADDATILGISID"
     gene            532..1671
                     /locus_tag="PLG78_18075"
     CDS             532..1671
                     /locus_tag="PLG78_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25087.1"
                     /translation="MRTVIAITTLIVACARPFPMQEREQNFLSALLLYAGFSSCSTPA
                     FSGIRAVDTPGNMGMGAAIAVSRCVIYITYLDNGTGNIKLARSYDGGNSFVLSVVGPT
                     GTNPSIAVSGSTVHIAYFFTAGADLRYARSTDGGETFTAVTLDSVGSVGDQSSVAVAG
                     SNVYIAYYDSTNIDLKLAKSTDSGSTFSISTVDSAGSVGQQTAIAAEGSEVYITHYES
                     AAFDLKLARSIDSGANFINSVIDSAGSVGQYSAVLKDSGNVYVSYYDSGGGDLKLGKS
                     TNAGASFNVFSAETANNVGNYSTAAVLGNTIYVAHYDATNFDLRLLRSYDQGMNFTAS
                     TIETTGDVGQYASVAAESNNLYVAYYDNTNQDLRFARSSNLGVSF"
     gene            complement(1799..>2478)
                     /locus_tag="PLG78_18080"
     CDS             complement(1799..>2478)
                     /locus_tag="PLG78_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25088.1"
                     /translation="LMSQALTLDRTQALLEESDSAQQRQPASPPLTLNPAMFVIQNEV
                     RKYAREMGIRDEATSEWLARNGPSLVDGRNPQEVILGLLEPEYFSQVYTLDARARPKF
                     ETMLKAIQKIKEESEQNKSAFAVVYIPSDDRYDREKFNLMREYGYSMNEEWLDGRVLS
                     DIEIERWMSNHQTPYLSMLSPFRKSREPLTFPRDQHLNKRGHEVMASAVAQFLEGNFA
                     SRLQKRN"
BASE COUNT          561 a          626 c          654 g          637 t
ORIGIN      
        1 gtgcgaaact ccggcagcgc ttcttcaaaa cttgaatgcg gcgcttgtaa gcgggtctgc
       61 aggaagtcca gtcacggcgc agtatctcat cgttgcccct gaccggagga gcgctactct
      121 agcatctgcc ggacatcccc cgccgattct ttccaggaat ggtgagacga ttcaaatgct
      181 cagcagcgga ccaatgctcg gggttgtgga tccaattcag gcgcgattct ttcgcacgga
      241 gctgcaggca ggcgatcgcc tttttctcta tacggacggt cttgttccga caaagcagga
      301 tgccccagat cttagaatgc atccccttca taaagcaatc atcaatagtg ggaatacgga
      361 ccttgagtcg caattgaaag agattgtgcg ttccctgcgc gcaggtcagg ccggttttgc
      421 agatgatgcg actattttag gaatatctat cgattagtcc tgccctgaaa gtcctggtgc
      481 agtgatgcga aagaacttga ctgaactgct tccgcgttcc agatttcgat catgcgcacg
      541 gtgattgcaa tcacaacgct gattgtggct tgcgctcgcc ctttcccaat gcaagagcga
      601 gagcaaaatt tccttagcgc tctgcttttg tacgcgggct tctcttcctg ttccacaccg
      661 gcgttttcag gaatacgcgc agtggacacg ccgggcaata tgggcatggg cgctgcgatt
      721 gcggtttcgc ggtgcgtaat ctacatcact tatctggaca acggcaccgg aaatatcaaa
      781 ctcgcgagat cctatgatgg aggaaattcg tttgttctct ccgtggtcgg acctacggga
      841 accaatccat ctatcgccgt gagcggcagc acggttcata tagcctattt ctttacggcg
      901 ggagcagatc ttcgctatgc gaggtctaca gatggagggg agacattcac ggcggtcact
      961 cttgattcgg ttggaagcgt aggagaccaa tcatctgttg ccgttgcggg cagcaatgtt
     1021 tacatcgcgt actatgacag caccaacatc gacctcaagc tcgcgaagtc cacggactcc
     1081 ggatctacgt tctccatatc aactgttgat tcagcgggga gcgtgggcca gcaaacggcg
     1141 attgcggcag agggtagcga agtctacatc actcactacg aatcagcggc atttgacttg
     1201 aagcttgcgc gttccatcga ttccggcgcg aattttatca attccgtaat cgattctgcg
     1261 gggagtgtgg ggcagtattc tgcagtgctc aaggatagtg gaaacgtgta cgtgagctac
     1321 tatgattcgg gaggaggcga tctgaagctt gggaaatcca caaatgccgg cgcatctttc
     1381 aatgtttttt cggcagaaac ggcgaataac gttgggaatt actctacagc ggctgtgctc
     1441 gggaatacaa tttatgttgc gcactatgac gcaaccaact tcgatttaag gcttctgcgt
     1501 tcttacgatc agggaatgaa cttcacggct tccacaatcg aaacaacagg cgatgtggga
     1561 caatacgcgt ctgtggctgc ggaatccaat aatctgtatg tggcttacta cgataacacg
     1621 aaccaggacc tgcgattcgc gcgttcttcc aatctgggtg tgtctttcta gatcaggcgg
     1681 gggtcgcggg tctagggttg catagaatct ctccgcttga atccgttccg ccgtggggcc
     1741 acgcctccaa cacaagttgt gaggcaatct tgcaccggct ctacgtggaa caatagagtt
     1801 agtttcgctt ttgcagccgc gatgcaaaat tcccttccag aaattgagcc accgctgaag
     1861 ccataacctc atggcctcgt ttgttcaaat gctgatcccg cggaaaggtg agtggttcgc
     1921 gcgatttacg aaatggactg agcatactga gatacggggt ctgatgattt gacatccacc
     1981 gctcgatctc aatgtcggag agtacccggc catccagcca ttcttcattc atcgaatacc
     2041 catattcacg catcaggttg aatttttccc tgtcatagcg atcatccgac ggtatgtaaa
     2101 cgacagcaaa ggccgatttg ttctgctccg actcctcttt gattttctga atcgctttta
     2161 gcattgtttc aaatttggga cgcgcgcgcg cgtccagggt atagacctgt gaaaaatact
     2221 ccggttcaag taaaccaagg atcacttctt gtggattgcg tccgtcgaca agtgaagggc
     2281 catttcgtgc tagccattcg cttgttgctt catcccggat ccccatctcc ctggcgtatt
     2341 ttctgacctc gttttgaatc acgaacatcg cgggattcag cgtgagtgga ggggaagccg
     2401 gttgtctttg ttgagcagaa tcgctttcct caagcagtgc ttgagtgcga tcgagtgtga
     2461 gtgcctgact catgagaa
//
LOCUS       DAOQJL010000570         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30879_length_2478_cov_4.005365, whole genome shotgun sequence.
ACCESSION   DAOQJL010000570 DAOQJL010000000
VERSION     DAOQJL010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30879_length_2478_cov_4.005365"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..528
                     /locus_tag="PLG78_18085"
     CDS             67..528
                     /locus_tag="PLG78_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE25089.1"
                     /translation="MAIHILRETELIPISQAEAWDFFSRPENLKTITPPGIGFDIQGN
                     PPEKIYPGLMITYIVKPLFGIPLTWVTEISHVREGEYFVDEQRIGPYKIWHHEHHFAA
                     KGASTEVTDIVTYALPFGILGKIAHAIIVKPRLRAIFDFRREKIRSLFPSK"
     gene            complement(535..1020)
                     /locus_tag="PLG78_18090"
     CDS             complement(535..1020)
                     /locus_tag="PLG78_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNE25090.1"
                     /translation="MTGIDYQVNPLWQFLRETYGFEKAFAFFGPYAGASIRVEIVDSN
                     TIVSSMDLVMTNTNYVGSHFGGSLYSMCDPFYMFILMENLGPDYIVWDKSASIDFIRP
                     GRGLVRARFHISKEEIEKIRDQVAKNRKMDWTADCEILDESGTVVARVHKVLYVRKNK
                     K"
     gene            1111..>2478
                     /locus_tag="PLG78_18095"
     CDS             1111..>2478
                     /locus_tag="PLG78_18095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE25091.1"
                     /translation="MVSPVRLVGLFLIIFGFFAAAGTALSRRPAPPSLQRAKVFYDAE
                     LPERGDHEILDFLAASGDPADAWCLALAEHAESHSNVSDAMLKQLNEPGQLRYKTLFA
                     FVANLNQVSTVQESWPLLRDAGCVLERLHRDAEAIALLKKAEQIHPDPDQRLDILHLV
                     HSLGWKTDFRALMADPLYYEEADPFLRMNYAQSRGEFVLAGYWLIRHQIESYQPGTMI
                     AAAGAGLCWLLLMLQLGRVQNWSWKYTSLVPFALILGGLSAYATVFEISIEQRFFVFE
                     RYSPHWLLNLIYAVLGIGLREETLKLLFSLPVILLARKAEPIVVLALASCTGLGFAIE
                     ENTQYYAGDWGAVFARFLTANFAHMMWTGMAGLAFTDALKGRTSWGEFFEQLAFVIAM
                     HGLYDFFIIDSTIADLAFFSSLIFIWVARSFFHNAHASRGGQKDKPPLLVTFTSMLGA
                     TAGICF"
BASE COUNT          598 a          655 c          581 g          644 t
ORIGIN      
        1 agacgcgttt cattcactct gcaatgaacg agtagccgga ttcgtccgat tccagtatag
       61 tatcctgtgg caattcacat tctgcgcgaa acggaactca tccctatatc tcaagcagaa
      121 gcatgggatt ttttctcacg cccggaaaac ctcaagacaa tcactcctcc tggaatcggt
      181 tttgatattc aggggaatcc tccggagaaa atctaccccg gcttgatgat cacatacatt
      241 gtaaaaccgt tattcggcat tcctctgacc tgggttacgg aaatatctca tgttcgcgaa
      301 ggagaatatt tcgtggacga gcaaagaatc ggaccctaca agatctggca tcatgaacat
      361 catttcgcgg caaaaggagc gtctactgaa gtaacagaca tcgtaaccta tgccctgccg
      421 tttggaattc taggaaagat agctcacgcg atcattgtga agccgcgtct tcgcgccatc
      481 tttgattttc gtagagagaa gatcagaagc cttttccctt ctaaataaaa aaggctactt
      541 cttgttcttt cgcacgtaga gtactttgtg aacgcgagcc acaactgtcc cggattcatc
      601 tagaatttcg caatctgcag tccaatccat cttccgattc tttgcaactt gatcgcgtat
      661 cttttcaatt tcctctttcg aaatgtgaaa tcgtgcacgg accagacctc gacccggtcg
      721 aataaaatca atgctagcac tcttgtccca tacaatatag tccggtccca gattctccat
      781 cagaatgaac atgtagaaag gatcgcacat ggagtacagc gatccgccaa aatgagaacc
      841 tacgtaattg gtatttgtca tcaccagatc catgctgctt acgatcgtat tggaatctac
      901 aatctcaaca cggatactgg cgcctgcata agggccaaaa aacgcgaatg ctttctcgaa
      961 tccgtacgtc tccctgagga attgccagag aggattcacc tgatagtcta ttccagtcat
     1021 aacccctcct ggctttccgc ctggtgagcg caatccaatt gcaagccagg ttctacttga
     1081 ccgcaccccg catctctgag aagcaaacgt gtggtttctc ccgttcggct ggttggtcta
     1141 tttctgatca tctttggctt ttttgccgcc gcaggcaccg ccctttcgcg tcgcccggct
     1201 cctcccagtc tgcagagagc gaaagtgttt tatgatgccg agctacccga gcgcggagac
     1261 catgaaatcc tggatttttt ggcagcatcc ggagatccag cagatgcctg gtgtcttgcc
     1321 ctggcggagc atgcagaatc gcacagcaat gtttcggatg cgatgctcaa acaactgaac
     1381 gaaccgggac aactgagata caaaacgtta ttcgcctttg tggccaatct aaatcaagtc
     1441 agcacagttc aagaaagctg gccattgcta cgggatgcgg gatgcgtgct tgagagacta
     1501 caccgggatg cagaagcaat cgcgctgctc aaaaaggcag agcaaattca ccctgatccc
     1561 gaccagagac tcgatatcct tcaccttgtt cacagcctgg gatggaagac agactttcgc
     1621 gccctgatgg cagatcctct gtactacgaa gaggcggatc cattcctgcg tatgaactac
     1681 gcccagagta gaggtgagtt cgtccttgcc ggatactggc tcattcgtca tcaaatagag
     1741 tcctaccagc ccgggacgat gatcgcagcc gcgggtgccg gtctatgctg gctgctgttg
     1801 atgctgcagc tgggccgggt gcagaactgg tcatggaaat acacatccct ggttccgttt
     1861 gctttaatcc tgggcggact cagtgcatat gcaacggtct ttgaaatcag cattgaacag
     1921 aggttcttcg tattcgaacg ttattcgcct cactggttgc tcaatctaat ctacgcggtc
     1981 ctgggcattg gtctgcgcga agaaacactg aagctgctgt tttcattgcc agtgatatta
     2041 ctggcccgta aggcagaacc catcgtggtt ctggccctgg cttcctgtac aggactcggc
     2101 tttgccatcg aagaaaacac tcagtactat gcaggggatt ggggagccgt gtttgccaga
     2161 tttctcactg cgaatttcgc acatatgatg tggaccggca tggcgggtct tgccttcacg
     2221 gacgctctca aaggacgtac ttcctggggc gaattcttcg aacaacttgc cttcgtgatc
     2281 gcaatgcatg gattgtatga tttctttatt atcgattcta cgattgcgga tcttgccttc
     2341 ttctccagcc tcatcttcat ctgggtagca cgctccttct ttcacaacgc acacgcttcg
     2401 cgtggcggac aaaaagataa acccccgcta ctcgttacat tcacatcgat gctgggtgcc
     2461 acggccggca tttgtttt
//
LOCUS       DAOQJL010000571         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30882_length_2478_cov_3.592241, whole genome shotgun sequence.
ACCESSION   DAOQJL010000571 DAOQJL010000000
VERSION     DAOQJL010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30882_length_2478_cov_3.592241"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2478
                     /locus_tag="PLG78_18100"
     CDS             <1..>2478
                     /locus_tag="PLG78_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25092.1"
                     /translation="IGLITADNQRAYEEFLSGHYESAQKLFANAGENARSHGFLDQAR
                     RNYINRYQALFALFESGDDALPRMKGALADLDEFRSVYREKAKEEYISQKQTEDPAFE
                     YEPAIDDPILESQLTRRLQDFVVVEANLHYYSGYSLDRRATSPADLTQARAHYTKALE
                     LYDRLIAGQQDEATSPVHFRVRLNRGVILSRLGRIEQAMNVLSRMIEELEEFALSGEE
                     WFARRALANLAAARFFERGRPSDAALAREQYIRAAEILTEDPTVRKGLIGMQEFYEDA
                     ADFFASSGDPQKANQLLEQSWVYALQEDYLRYPISFESTALTETYEKYLSASRTARAL
                     AVREQSLRFGRKDSKVAREEKMRALASLETARESMRSAVPLHEAFLSSPVSSPDLRKG
                     QWMIRLFYRGHTLSVFAFLPGFAYYREDGNPEAALKTILNRLPRRVPSALLVIADQET
                     WSLNLEAAIQSARPDLPVPLFVTRMSDPGVLYSEIRPELNQFNTLDANSDFEASDVFI
                     VEREFADDFFAIENGRFQARQFLQKRHNASMAVTDEPSRNFNKIRTAYELFRAGGVGS
                     LVIRQSKGPLPAQNQKLTGQGFRVMGSEGFRTDAVMGNIRARYAAALDQAGQFFSARE
                     FRRAAEQYRLASSLAASMGEKGKYLEARLKSASALMTVDAPAADRAFAQLLAGELDAA
                     QKSRVHAEGVKARFRTGRFKEAEVAARVWAEAFPAEKSQIDQQIRLVVFLRNLESENA
                     SDKFHDGSFATEFRALLSDLAQPEVAPSAIDLLLRRGFHSEAASIARLQRPAERKAAL
                     ARINFSQRLAEGKIPQEAPRDVFDENEP"
BASE COUNT          579 a          641 c          675 g          583 t
ORIGIN      
        1 gattggtttg atcactgcgg acaatcagag ggcatacgag gaattcttgt cgggtcacta
       61 tgaatcggcc caaaagttat ttgcgaacgc gggcgagaac gctcgctctc acggtttcct
      121 ggatcaggcc cgcaggaact acatcaatcg ctaccaggct cttttcgctt tgtttgaaag
      181 cggtgatgac gctctccccc gaatgaaggg tgcgcttgca gatctggacg aatttcgatc
      241 tgtatatcgc gagaaagcga aggaagaata catctcccag aagcagacgg aggacccagc
      301 cttcgagtat gagccagcca ttgatgatcc gatcctggaa tcgcaattga cccgtcgcct
      361 ccaggacttt gtggtcgttg aggccaacct tcattactac tccggctact ctctcgatcg
      421 ccgcgcgaca agtcctgcag atttgacgca ggcgcgcgct cactacacga aagcacttga
      481 actctatgat cgtttgatcg caggacaaca agacgaagct acctctcccg ttcactttcg
      541 cgttcgcctg aatcgcggag tcattctttc aaggctcggg cgcatcgagc aggccatgaa
      601 tgtactttcg cgcatgatag aggagctaga ggagtttgct ctttcgggag aggaatggtt
      661 tgcgcgtcgt gctctggcaa acctggccgc tgctcgattc tttgagcgcg gaaggccatc
      721 agatgcagct cttgcacgcg agcaatacat tcgcgcagcg gaaattctaa cagaagaccc
      781 gactgtgcga aaagggctga tcgggatgca agagttctac gaagatgcag ccgatttctt
      841 tgcttcgtcg ggggatcccc aaaaagcaaa ccagcttctg gaacaatcct gggtctacgc
      901 tcttcaagag gactatcttc gctatcccat ttcttttgaa tccacggcac ttacagaaac
      961 gtacgaaaag tatttgagtg cttctcgcac tgccagagca ctcgcagtcc gcgaacaatc
     1021 gctacgattt gggagaaagg attcgaaagt cgcgcgagag gagaaaatgc gcgcgctcgc
     1081 ttctcttgaa actgcccgcg aatctatgcg gtctgctgtt ccgttgcatg aagcgttcct
     1141 gagttcacct gtctcttctc ctgatctccg caaagggcag tggatgattc gtcttttcta
     1201 cagaggacac actttatccg tgttcgcgtt tttgccagga ttcgcctact accgcgaaga
     1261 cgggaacccg gaagcagccc tgaaaacgat tctgaacagg ttgcctcgaa gagtaccatc
     1321 tgcccttctc gtgattgcag atcaggaaac ctggtctcta aacctggaag cagcgattca
     1381 gagtgcccga ccggatctgc ctgtccctct gtttgtaacc aggatgagcg acccgggtgt
     1441 cctgtattca gagattcgtc ctgagctcaa tcaattcaat acgctggacg cgaattcgga
     1501 ttttgaggcc tcggatgtgt tcattgtaga gcgtgagttt gcggacgatt tcttcgcaat
     1561 tgaaaatggt cggttccagg cgcgacaatt tctgcagaaa cgacacaatg cgagcatggc
     1621 ggtgacggat gaaccctctc gcaatttcaa taagatacgt actgcttacg aattgtttcg
     1681 cgctggagga gttggctcgc tggtcattcg ccagagcaaa gggccgctgc ctgctcaaaa
     1741 tcagaagctg accggccagg gatttcgcgt catgggatct gagggctttc gcacggatgc
     1801 tgtgatggga aatatccgtg cgcgctatgc agctgctctg gatcaagcag gtcagttctt
     1861 ttccgcgcgc gagttcaggc gcgccgccga gcagtacaga cttgcttctt ccctggcagc
     1921 ttctatgggc gaaaaaggca agtaccttga ggccagattg aagtctgcat ccgcactcat
     1981 gacggtcgat gcgcccgcgg ctgatcgcgc tttcgcgcaa cttctggcgg gggaactgga
     2041 cgccgcacaa aagtcgaggg ttcacgcaga aggagtgaag gcgcgttttc ggactggacg
     2101 atttaaggaa gcggaagtag ccgcacgagt ctgggcggaa gcatttccgg cggagaaaag
     2161 tcagattgat caacagatcc gtctggttgt gttcttgcga aatctggaga gcgaaaacgc
     2221 ttcggacaaa tttcatgatg gatcatttgc cacagaattt cgtgcgttgc tttctgatct
     2281 cgctcaacca gaggttgcac cctctgcgat tgatttgttg ctcaggcgcg gttttcactc
     2341 agaagcagct tccattgcca gattgcagag acctgcagaa aggaaggccg cacttgcgcg
     2401 aatcaacttc agtcaaaggc tcgcggaagg aaaaatacct caggaagcac cccgtgacgt
     2461 tttcgacgaa aacgaacc
//
LOCUS       DAOQJL010000572         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_30910_length_2477_cov_4.209331, whole genome shotgun sequence.
ACCESSION   DAOQJL010000572 DAOQJL010000000
VERSION     DAOQJL010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30910_length_2477_cov_4.209331"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLG78_18105"
     CDS             <1..1237
                     /locus_tag="PLG78_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25093.1"
                     /translation="YCSSGSSKKDSDDDKTVFGDCLDGKITVRGEAPIYTSMDAAEGK
                     AKEDACRTAVEKCIGTQIASSSGVGDGQSIGNEIFTKANGMCRNGKILERNEYPFDTF
                     KMLRLTIRYEVSESELKSQIDQMQKLAGNPKVMILIREEYNIAGQGKRVESFTSRNAI
                     AAAALRDYLVRKDYTVIDASSISLGGMNEEVAAENPSQFPEDVKERAAKAGADVLIIG
                     SLESNPQASIDLSDETMKLKMKSYKATGNVTLMTLWGKGKVLGEFNTPMGGAQTTDLS
                     AAQQSMKRYAVGSAPDPIKSPGGIAKEINRRLSDQWSELTKNNVIQISISGLNQKSAG
                     MFRDDLKGKTSVRDVNPLSESDGSQEWEVTYPGRSFALADTISWNRDNPKIFQVIKES
                     GKKPTVLSVTRGEIKIRFD"
     gene            1291..1764
                     /locus_tag="PLG78_18110"
     CDS             1291..1764
                     /locus_tag="PLG78_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNE25094.1"
                     /translation="MKPASTYFRSAQIARFAVILLFFTACDALRTQHLRTEVIELGEY
                     KIRAEIASTPRERERGLMYRTSIGEEEGMLFVFPKASPQAFWMKNTLIPLDVGYFDEQ
                     GFLIEYFTMTPDDGVRTYPSSEPALYALEMKAGWYAKRGLRKYTKLVFPRSLEGL"
     gene            complement(1771..2346)
                     /locus_tag="PLG78_18115"
     CDS             complement(1771..2346)
                     /locus_tag="PLG78_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE25095.1"
                     /translation="MPDTSPTIVLVRHGKPTMRVASRPWQWINSHEVNRRMDAYDQAS
                     LDPHWNGPRAPRIEGHHSISSDLVRAIETAKLIVGEDPREISPLFREVPLPRFRRNVR
                     LPSMALVTMSRLGWFWGFMEGEESRRQTNARVREAADHLEDRVQSHRKVALFSHGFFL
                     WLLSRELGERGWTSEKKGPFSYLETAEFLRL"
BASE COUNT          671 a          572 c          629 g          605 t
ORIGIN      
        1 gtactgttcc tcagggtctt cgaaaaaaga ttctgacgat gataagactg tattcggcga
       61 ttgtctcgat ggcaagatta ctgtgcgcgg tgaagcgcct atttatacca gcatggacgc
      121 cgcagaaggt aaggcaaagg aagacgcatg ccgcactgca gttgaaaagt gcattggcac
      181 tcagattgca agttcctccg gtgtgggaga tggtcagtcc atcggaaatg aaatcttcac
      241 caaagcaaac gggatgtgtc gcaatggcaa gatcctggaa agaaacgaat atccttttga
      301 tacattcaag atgcttcgtc ttaccatccg ctacgaggtc tctgaatctg aactgaaaag
      361 ccagattgat cagatgcaaa agcttgcagg caatccaaag gtaatgatcc tgattcgcga
      421 agaatacaac atcgcaggtc agggaaagag agtagaatca ttcacttcta gaaatgcaat
      481 cgccgcggca gcacttcgcg actacctggt tcgcaaagac tataccgtga ttgatgcttc
      541 ttctatttct cttggaggaa tgaacgaaga agtggccgca gaaaatcctt cgcagtttcc
      601 agaagacgta aaagaacgcg cagcgaaagc gggggcggat gttctcatca ttggaagcct
      661 ggaatccaac cctcaggcat ctattgacct cagcgatgaa accatgaagc tcaaaatgaa
      721 gtcttacaaa gcaacaggca acgtgactct tatgactcta tggggcaaag gaaaagttct
      781 cggagaattc aatactccca tgggtggtgc gcaaacaaca gatttgtccg cagcgcaaca
      841 atccatgaag cgatatgcag ttggttctgc tccagatcca atcaaatcac ccggtggaat
      901 tgcaaaagaa atcaatcgca ggctttcaga tcagtggtcc gagcttacaa aaaacaatgt
      961 aattcaaatc agcatttcag gattgaatca gaagtctgcg ggtatgttta gagatgatct
     1021 gaagggcaag acttcagttc gtgatgtcaa tccactcagt gaaagcgatg gaagccagga
     1081 atgggaagtg acttatcccg gtcgttcttt tgctctcgcg gacacgattt cctggaatcg
     1141 agacaatcct aaaatttttc aagtaatcaa agaatcaggc aagaagccaa cggttctcag
     1201 cgtcactcgc ggagagatca aaattcgatt cgattgaaac tttgatgccg gccattggaa
     1261 acattggccg gctgtcgatc gtctgacaaa atgaaacctg cttctaccta ctttcgatcc
     1321 gcacagattg cacgcttcgc ggtcattcta ctctttttca ctgcctgcga tgcgctgcgc
     1381 actcagcatt tgcgcacgga agtgattgaa ctgggtgaat acaaaatccg ggctgagatt
     1441 gcttccactc ccagggagag ggaacgtggt ttgatgtatc gcacttcgat tggagaagag
     1501 gagggcatgc tgtttgtttt tccaaaagca tctcctcagg ctttctggat gaagaatact
     1561 ctcattccgc tggatgtagg ctattttgac gagcagggct tcttgataga atacttcaca
     1621 atgacaccgg atgatggagt gcgcacttat ccttcttctg aaccagcgtt atacgctctt
     1681 gagatgaagg caggctggta tgcgaaacgt ggactgagaa agtacacgaa gcttgttttc
     1741 ccgcgtagcc tcgaaggtct ctagcagaaa ttacagccgc aggaattctg ccgtctccag
     1801 ataagaaaaa gggccctttt tttcagaagt ccaaccgcgc tcaccgagct cccgagataa
     1861 caaccataga aagaagccat ggctgaagag tgctactttt ctgtgggatt gaactctatc
     1921 ttctagatga tctgctgcct cgcgcacccg cgcattggtt tgtctgcgcg attcttctcc
     1981 ttccataaaa ccccagaacc atccaaggcg tgacatggta acaagcgcca tggatggaag
     2041 gcgtacattg cgacggaatc ttggaagagg tacttctcga aagagaggac tgatttcgcg
     2101 cggatcttct cctacaatca gtttggccgt ttctatggcg cgcacaaggt ctgaggagat
     2161 ggaatgatga ccttcaattc gcggagcacg gggtccattc cagtgaggat ccagactggc
     2221 ctggtcgtac gcatccattc tgcgattgac ttcgtgagag ttgatccact gccaggggcg
     2281 cgaagcaacg cgcatcgtgg gttttccgtg acgcacgagc acgatcgtag gagacgtatc
     2341 gggcatgcgt cagctttgtt cagagttcca ggacgtcgac tacaacgatg gtggtatcat
     2401 ctgcgagatt gcgatctcct gaatagtcca ggatttcaag gattgcgttt gtagtgacct
     2461 gatgcgcggt actttct
//
LOCUS       DAOQJL010000573         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31226_length_2461_cov_3.714879, whole genome shotgun sequence.
ACCESSION   DAOQJL010000573 DAOQJL010000000
VERSION     DAOQJL010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31226_length_2461_cov_3.714879"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..780
                     /locus_tag="PLG78_18120"
     CDS             121..780
                     /locus_tag="PLG78_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25096.1"
                     /translation="MIRVNLVTDIRPGLPQTGQERFRFLGLTPEGLVLLSHAGKLIHA
                     RMDGKPDILPAPGDFFTAKVKQAGDQLELSLLKADSSKSEARAPEIPREILGVFAELL
                     HKDDLPAAEILRLHAYPMSDFLVVPGTLQEKKRRGSLQSGHGKLTGPYYFLLRTRFDK
                     LGEIGILLYADNPDFSGVRALLTAPNEYVRARLAERLRELREIDGRVISNSTHVVDRS
                     A"
     gene            780..1049
                     /locus_tag="PLG78_18125"
     CDS             780..1049
                     /locus_tag="PLG78_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNE25097.1"
                     /translation="MEEQKKAAALRFDPATDRAPRIVASGVGTLAEDIVRTAEESGVK
                     VVEDGNLAGFLLDLPAGIEIPENLYKAVSSIFAMLIRLEGEESSL"
     gene            1046..2227
                     /locus_tag="PLG78_18130"
     CDS             1046..2227
                     /locus_tag="PLG78_18130"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE25098.1"
                     /translation="MKKLQVSELKPGMVFDQPVYIDSSNILLQSGQELLAKDIERLQK
                     WGIHEVMTAGTLIQSGGATGDSLPPPVSAKDPEIQKLESDYEAFRKARFNIKSMVQQG
                     SDILTANMHALVEGKPFDNHALLNLAGKIVDEYHARRFFLLSFQGMTIKAIPQVYHNI
                     HAACYAVAIAQSQNFTRPRTQELVFSILAMDAGMYQMPAHVREKSSALADPERNAVKS
                     HTILGNQLLLKGGKVKPALANIALQHHEHFDGTGYPQNLKAAQIDELARIALIVDSYT
                     AMIEKRNYRPGMLPYDAMRTMLSQDTARYDPKLLRAFLGKVSIYPVGSIVQLSDKRLA
                     LTLGCRPEKPLRPLLRLLRDESGLPYNKATFVDLFVSSELYIVRAVDPSVTGVDLEAE
                     I"
     gene            2251..>2461
                     /locus_tag="PLG78_18135"
     CDS             2251..>2461
                     /locus_tag="PLG78_18135"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNE25099.1"
                     /translation="MAWEFLRANGHELLAHNFHAKFAEIDLITRDRQGIIHIVEVKAY
                     RGETHPLTAITRERENLMKKAAGVWM"
BASE COUNT          657 a          613 c          649 g          542 t
ORIGIN      
        1 agaccacaag ggatatggta caaaagcgca cagggacgaa atcaagcgga acggcgtaac
       61 caggcttcac aggcagtcat atggcatcat agaacagccg atgcttgatt tcggagacca
      121 atgattcgcg taaatctggt tacagacatt cgtccgggac tgccgcaaac aggccaggaa
      181 agattcagat tccttggtct aacgcctgag ggactggtac tcctcagcca tgcaggtaaa
      241 ttgattcatg caaggatgga cgggaagcca gacatactgc ccgcaccagg agacttcttt
      301 accgcaaagg tgaagcaggc gggagaccag ctcgagctct cgcttctaaa agcggactcc
      361 agcaagtcag aggcccgcgc gcccgaaatt cctcgggaga tactcggggt tttcgctgaa
      421 ctactgcata aagacgacct acccgcagct gaaatcctga ggctccatgc ataccccatg
      481 tccgatttcc tcgtggtgcc aggcactctg caggaaaaaa agagaagggg atcgctccag
      541 agcggccacg ggaagctcac agggccgtat tactttctgt tgcgcaccag gtttgacaaa
      601 ctcggcgaaa tcgggattct actctatgca gacaatccag acttcagtgg agtaagagcg
      661 ctgctgactg ccccgaacga atacgtgcgc gctcgtctcg ccgaacgtct cagagagctt
      721 cgcgagattg acggccgggt gatttcaaat tcgacgcacg ttgtggacag gagcgcataa
      781 tggaagagca gaagaaggcg gccgctttac gcttcgaccc ggccacagac cgggctccgc
      841 gcatcgtggc gagcggagta ggcactcttg cggaagatat agttcgaacc gcagaggaga
      901 gtggagtaaa agtggtggag gacggaaacc ttgcaggatt tctacttgat ctgcctgcgg
      961 gaattgagat acctgaaaac ctgtataaag ctgtgtcgtc catattcgca atgctcattc
     1021 ggcttgaggg agaagagtcc tccctatgaa gaagctccag gtttctgaac taaagccagg
     1081 tatggtattt gaccaaccag tctatattga ttcatccaac atactgttgc agagtggtca
     1141 ggagttgctg gccaaagaca ttgagagatt gcagaagtgg ggcattcatg aagtcatgac
     1201 tgccggcacc ctcattcagt caggcggggc aactggtgat tctcttcctc ctccggtttc
     1261 agcaaaggat cctgaaattc agaaactgga aagcgattac gaagcatttc gcaaggctag
     1321 attcaatatt aaaagcatgg tgcagcaggg atcggacata ctgactgcga acatgcatgc
     1381 tcttgtagaa ggtaaaccat tcgacaatca tgccctgctg aatctggcgg gaaagatcgt
     1441 ggacgaatat cacgctcgcc gtttctttct tttgtctttt cagggaatga caatcaaagc
     1501 aattccgcag gtgtatcaca acatccacgc tgcctgctac gcggtggcca tcgcacagag
     1561 ccagaacttc actcgacctc gcacgcaaga acttgtcttt tctattctcg ctatggatgc
     1621 aggcatgtac caaatgcccg cgcatgtcag agaaaaatca agcgcgcttg cagatccgga
     1681 aagaaatgcg gtaaagagtc atacaattct gggaaatcaa cttttgctaa aaggcgggaa
     1741 ggtgaagccc gcacttgcaa acattgcatt gcaacatcat gagcattttg acggaaccgg
     1801 ttatccacag aatttgaagg cggcgcagat agatgaactc gcacgcattg ccctcatcgt
     1861 ggattcgtac acagccatga tagagaaacg caattatcgt ccgggaatgc tcccttacga
     1921 cgcgatgcgc accatgcttt cccaggacac agctcgctat gatccaaaat tactcagagc
     1981 gtttctgggc aaggtttcta tctatccagt tggttctatt gttcaattga gcgataaaag
     2041 actggccctc actctgggtt gcaggccgga gaagccactt cgtccgttgc tccggcttct
     2101 acgcgatgaa tcgggcctcc catacaacaa agctacgttc gtcgatctgt tcgtttcatc
     2161 tgagctctac atcgtgcgcg ctgtggatcc atctgtgaca ggtgtggatc tggaagcaga
     2221 gatctgaaat tcagacatcc agcagaagaa ttggcctggg aatttctccg ggcaaacgga
     2281 catgagcttc ttgctcacaa ttttcacgca aaatttgcgg agattgacct gatcacccgt
     2341 gaccggcaag gaatcattca tatagttgaa gtcaaggcgt acagagggga gactcatcca
     2401 ctcactgcga taaccaggga gcgcgaaaac ctcatgaaaa aggctgccgg ggtgtggatg
     2461 g
//
LOCUS       DAOQJL010000574         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31459_length_2450_cov_4.684760, whole genome shotgun sequence.
ACCESSION   DAOQJL010000574 DAOQJL010000000
VERSION     DAOQJL010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31459_length_2450_cov_4.684760"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLG78_18140"
     CDS             <1..229
                     /locus_tag="PLG78_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25100.1"
                     /translation="LGRLGYKYEISDKAAVVAAVVHEQNEGKRDADSTTLELGFQWEA
                     NDGLTIGPGIVVGLDHHSKTPRFGAGLNVTF"
     gene            278..1033
                     /locus_tag="PLG78_18145"
     CDS             278..1033
                     /locus_tag="PLG78_18145"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25101.1"
                     /translation="MKDNANTLSKVPAVTLVFWILKILATTLGETAGDAVSMSLDLGY
                     FIGTAIFAVTFLVAAAVQIRTKQFHPVIYWITIIATTTVGTTMADLADRSLGIGYAGG
                     SSLLLFLLCTSLFIWHRVMGSISVDSINNWKSELFYWVTIMFSQTLGTALGDWTADSA
                     GFGYLGSAGIFGGALAVIALLYYATKISHTLLFWAAFILTRPLGAVVGDLLDKPLNEG
                     GLDLSRFAASAVLIALMACGIFFFKHRAAEETH"
     gene            complement(1041..1781)
                     /locus_tag="PLG78_18150"
     CDS             complement(1041..1781)
                     /locus_tag="PLG78_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE25102.1"
                     /translation="MALIVIIDSDLTQARALEGALAGLRHRILATQSAARGIELAQSG
                     NPDLILLNPENSQMDGIQILASLRKDPITKEIAVALVQRQPDAALLPRLRQLGVVDTI
                     SPGLTQDLLRKKILQSLVLAEKHKLDHNLKRANHIHVRRTPGRTDISILSGLKEFSFQ
                     EAKTVLNPFFVRLTKNDSMVLDIRAIPNINETELPLLQQIIDLVANEKLILIAGRHLG
                     LLIAETEIGETVPTFFTQEEAEQHLSAR"
     gene            complement(1836..>2450)
                     /gene="msrB"
                     /locus_tag="PLG78_18155"
     CDS             complement(1836..>2450)
                     /gene="msrB"
                     /locus_tag="PLG78_18155"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNE25103.1"
                     /translation="KAIVTEIVPIKVFYPAEEYHQDYYKKNPYSYKRYRSGSGRDEYL
                     ERVWGKQSTSKEKRMYTKPPEEELKKKLTPLQYEVTQHEGTERPFQNEYWDNHKDGIY
                     VDRVSGEPLFSSKDKYDSRTGWPSFTKPLDPANIVTKKDMSVGMVRVEVRSKHGDSHL
                     GHVFDDGPAPTGQRYCMNSASLRFIEKSDLEKEGYEEFAKLFDK"
BASE COUNT          554 a          626 c          626 g          644 t
ORIGIN      
        1 ccttggcaga cttggctata agtacgaaat ctcagacaaa gcggccgtcg tggccgctgt
       61 agtacatgaa caaaacgaag gcaaacgcga tgcggactcc accacactgg agctgggctt
      121 tcagtgggag gcgaacgatg gccttacgat tggcccgggc attgtggtcg gactggatca
      181 tcacagcaag acgccgcgct tcggtgcggg cctcaatgtt acattctaaa agtatgtaat
      241 tctacttata tgtagtacaa actaaaggaa ctctcaaatg aaagacaatg caaacactct
      301 aagcaaggta ccggccgtga ctcttgtttt ctggattcta aaaatccttg ccaccactct
      361 cggagaaacg gcaggagatg cggtgtctat gtccctggat ctaggatact tcataggaac
      421 tgcgatcttc gccgttacct ttcttgtcgc ggcagccgtg caaatccgaa ccaaacagtt
      481 tcatccggtg atctactgga tcacaatcat cgctactaca accgttggca cgaccatggc
      541 ggatctcgca gatagatccc ttggaatcgg gtatgcaggc ggatcgtctc ttttgctctt
      601 cttgctgtgc acttctctct tcatctggca cagagtgatg ggttcgattt ctgtggattc
      661 catcaacaac tggaaatcag aattgttcta ctgggtcacc attatgttct cgcagaccct
      721 cggcacggcc ctgggagact ggacggcgga ctccgcaggc ttcggatatc tgggaagtgc
      781 gggcatcttc gggggagcgc tcgcggtcat cgcactgctc tactatgcca cgaagatttc
      841 gcacactctc ttgttctggg cagcattcat tctgacaaga cctctgggtg cagtcgtcgg
      901 ggaccttctg gacaagccac taaacgaagg tggtctggat ctgagtcgat ttgccgcttc
      961 cgctgtgctg atcgctctta tggcctgcgg aatcttcttt ttcaaacatc gcgcggcaga
     1021 agaaacgcac tgaatgccgc ctaacgggcc gacaaatgtt gctccgcttc ttcctgtgtg
     1081 aagaaagtgg gcacagtttc tccaatctcg gtttcagcaa tcagcaaacc gagatggcgg
     1141 cctgcgatga gtataagctt ttcgtttgcc accaggtcga tgatctgctg caaaaggggc
     1201 aattctgttt cgttgatgtt tggaatggca cgaatgtcca ggaccatgga gtcattcttt
     1261 gtaagcctca caaagaatgg attgagaact gtcttcgctt cttgaaacga aaactctttg
     1321 aggcccgaaa ggatagagat gtctgtcctg ccgggagtgc gacgaacgtg gatgtgattg
     1381 gctcgcttca ggttgtgatc gagtttatgc ttttctgcga gaacgagaga ctgcaggatc
     1441 ttctttcgca aaagatcctg cgttagacct ggcgaaattg tatctacgac acccagctgt
     1501 cgcagacgtg gaaggagagc tgcatccggt tgacgctgca ccagagcgac tgcgatttct
     1561 tttgtgattg gatccttgcg aagcgaggcc agaatctgga tgccatccat ctgcgagttt
     1621 tctggattga ggaggatgag atccgggttt ccactttgcg ccagttcaat cccgcgcgct
     1681 gcggattgag tggccagaat gcgatggcga agaccggcca gcgcaccctc aagcgctctg
     1741 gcctgcgtca aatcagagtc aatgatgacg atcagggcca tgaggaacaa aggccccata
     1801 ttgccccaat ggtcaagtcg actctgatcc aagattcatt tatcgaagag ttttgcgaat
     1861 tcctcgtagc cctctttctc caaatcactc ttctcaatga agcgaaggga tgcagagttc
     1921 atgcagtacc gctggcctgt aggagcgggg ccgtcatcaa acacatgacc cagatgcgag
     1981 tcaccatgct tgcttctcac ttctactcgc accattccca cagacatatc cttctttgtg
     2041 actatattgg cgggatccag aggcttagtg aagctgggcc agccagtacg cgaatcgtac
     2101 ttatcctttg aactgaaaag aggctctccc gaaacgcgat cgacgtagat tccgtcttta
     2161 tgattgtccc agtattcatt ctgaaatggg cgctctgtgc cctcgtgctg cgtcacctca
     2221 tattgaagag gcgtcagttt cttcttgagt tcttcttctg gtggcttcgt gtacattctt
     2281 ttctccttgc tggtggattg cttgccccat actctctcca gatactcatc gcgccctgaa
     2341 ccggagcgat acctcttgta gctgtacggg ttcttcttgt aataatcttg atgatattct
     2401 tccgccgggt aaaacacttt gatcggcaca atttctgtaa cgatggcctt
//
LOCUS       DAOQJL010000575         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31639_length_2442_cov_4.186845, whole genome shotgun sequence.
ACCESSION   DAOQJL010000575 DAOQJL010000000
VERSION     DAOQJL010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31639_length_2442_cov_4.186845"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1007)
                     /locus_tag="PLG78_18160"
     CDS             complement(207..1007)
                     /locus_tag="PLG78_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE25104.1"
                     /translation="MKTRNLFMSGILISSFLVYFACQSLPAGLSGGASDEDMAKLGAQ
                     AFEELKKKTPASTDPAINAYVRCVANAVLKVTDDPTGVKDWEIVVFQDPTANAFALPG
                     GKIGVHTGLLPVATNQDMLATVLGHEIGHVIKRHGATRMQGGGLLEKGVEVAGNMILG
                     DSAYKEQAMAALGMGVNYGVVMPFTRSQESQADLVGLDLMAKAGFNPGQSILLWKNMS
                     ALGGKKQPAFLSDHPSNENRIKDLQGAMPKAMQQMAAANAQGRTPNCR"
     gene            1134..1439
                     /locus_tag="PLG78_18165"
     CDS             1134..1439
                     /locus_tag="PLG78_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25105.1"
                     /translation="MSDGSVLKKLSEKLAEWDQELAKLRQQATSSAGEQKANFDKIAQ
                     DLTAQTAQIKAKIDEMKNKNPDEIKAQAEDLLRTASGKVSEGLKNLAGFFESKAKKV"
     gene            1583..>2442
                     /locus_tag="PLG78_18170"
     CDS             1583..>2442
                     /locus_tag="PLG78_18170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE25106.1"
                     /translation="MHLDRLLEIIMDRVTSVMNAERSSLFLLDHKTDELYARVAQGMN
                     VSSVRFPNGVGLAGHVGKTGQTINIADAYKDPRFNRDFDKKTGFRTRAILSMPIKNTR
                     GKIIGVSQVLNKKGENEIFTRDDEGLLQAFSSIAAISLENALAYETINATMHTFEKFV
                     PRRYLNSIAREGLESIRIGNAEKVDVSILFCDIRGFTTLSEKMKPEDVLAFLNGYLVR
                     MGNPIAKNGGFVDKFIGDAIMAIFDTRDPSSAVRAGVGMLKELESYNRERRKNKLDEI
                     KIGIGIHFGAA"
BASE COUNT          631 a          657 c          623 g          531 t
ORIGIN      
        1 gtagattcgg gaagcgtgta ctggaattgc tgtgatcgag tcagccccag gctgccctgg
       61 actggaagaa gaaattctgg gatgggttcc actctatccg tgattggaat tcgggcgttt
      121 ctgcaaccac gcgcgtggca tttgcgtgcc ccttgagaaa tcaaacgggg ccgagagccg
      181 gccccgaatt caatacagcc gttcgcctaa cgacagttag gtgttctccc ctgagcattg
      241 gccgctgcca tttgctgcat tgcttttggc atcgcaccct ggaggtcctt gattctgttt
      301 tcattcgatg gatgatctga caggaacgct ggctgttttt ttccgccgag tgcactcatg
      361 tttttccaca atagaatgct ctggccagga ttgaatccgg ctttcgccat caagtccaga
      421 cccaccagat ccgcctgact ttcctgagaa cgagtgaaag gcataacgac tccatagttc
      481 acacccattc ccagtgcagc catggcctgc tctttgtacg cgctgtctcc gagaatcatg
      541 ttgcccgcaa cctcgactcc tttttcaagc aggccgccgc cctgcatgcg agtagcaccg
      601 tgtctcttga tcacatgacc gatttcgtga cccagaacgg ttgcgagcat atcctggttt
      661 gtggccactg gaagaagtcc cgtgtgtact ccaatctttc cgcctggcag agcaaacgcg
      721 tttgcagttg gatcctgaaa cactacaatc tcccagtcct tcactccagt tggatcatcc
      781 gtgaccttga gaactgcatt ggccacacaa cggacgtatg cattgattgc cggatccgta
      841 ctggccggtg ttttcttctt caattcttca aacgcttgcg cgcccagctt ggccatatct
      901 tcatcggaag ctccgcctga aaggccggcg ggcaacgact ggcaggcaaa gtacacgagg
      961 aagcttgaaa tcaagatgcc tgacataaat aaattacgag tcttcataaa cctcgcgtaa
     1021 cgcgccggcc gtttactgca aggttatttc gcggattggc ggcgtcaggc ccctggtttc
     1081 gatcttgacc tgcttcgcgc aaggtgaaac cctggggtac aggggtgcca catatgtctg
     1141 atggaagcgt acttaagaaa ctgtccgaaa aactggcaga gtgggaccag gaactggcca
     1201 agctgcgcca gcaagcaacc agcagcgcgg gcgaacaaaa ggcaaacttt gacaaaatcg
     1261 cccaggattt gactgcgcaa acagcccaga tcaaagccaa aatcgacgag atgaagaaca
     1321 agaatccgga tgaaatcaaa gcccaggcag aagatctgct tcgcaccgca tccggcaaag
     1381 taagcgaagg cctcaagaac ctcgcaggct tcttcgagtc caaagctaag aaggtctaat
     1441 gcgggattcg ctcgaacgca gggcagtcaa gcggcgggcg gaggatcggg caccggaaac
     1501 agtccggtac attaaccagc tcgaagcaca aaacacgcgc ttaaagagtc tcctgaacgt
     1561 ctccaaagag atgatgcagg agatgcatct cgatcgcctc ctcgaaatca tcatggatcg
     1621 agtcacgtcc gtgatgaatg cagaaagaag cagtctattc ctgctcgatc acaaaacaga
     1681 tgaactctac gcccgcgtgg ctcagggaat gaatgtaagc tccgtgcgat ttccaaacgg
     1741 cgtaggcctg gccggacacg tgggcaaaac tggccagacc atcaacatcg cagacgcata
     1801 caaggatcca agattcaatc gcgactttga caagaagacc ggctttcgca cgcgcgccat
     1861 tctcagtatg ccgatcaaga acacgcgcgg aaagatcata ggtgtttctc aagtattgaa
     1921 caaaaaaggt gagaacgaaa tcttcactcg cgacgatgaa ggcctgctcc aggcattttc
     1981 ttcgattgct gcgatctcac tggaaaacgc actcgcttac gaaacgatca acgctacgat
     2041 gcacactttt gagaaattcg ttccaaggcg ctacctcaat agcattgcgc gcgaaggcct
     2101 cgagagcatt cgaatcggga atgcggagaa ggtagacgta tccattctat tctgcgacat
     2161 tcgcggcttc actaccctct ccgaaaaaat gaagccggaa gatgtgctcg catttctcaa
     2221 cggctatctg gttcgcatgg gcaatcccat cgcgaagaac ggaggcttcg tagacaaatt
     2281 cattggggac gccatcatgg cgatctttga cacgcgcgat ccatccagtg cagttcgcgc
     2341 gggcgttgga atgctcaaag agcttgagtc ttacaatcgc gaaagaagaa aaaacaaact
     2401 ggacgaaatc aaaattggaa tcggcattca ttttggcgcg gc
//
LOCUS       DAOQJL010000576         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31715_length_2439_cov_3.102768, whole genome shotgun sequence.
ACCESSION   DAOQJL010000576 DAOQJL010000000
VERSION     DAOQJL010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31715_length_2439_cov_3.102768"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLG78_18175"
     CDS             complement(<1..283)
                     /locus_tag="PLG78_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25107.1"
                     /translation="MNCPYFQSGLPQKKGVVFALFLAVSGIPSCSKNAANLPGILFAP
                     GSATRPSVVLSSPSALTPNVPSDIPVYVEFDREMRPNETENAFTLSGSAP"
     gene            complement(280..1698)
                     /locus_tag="PLG78_18180"
     CDS             complement(280..1698)
                     /locus_tag="PLG78_18180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE25108.1"
                     /translation="MRRLRSMPVRARYAIIACSVLGITMAIYQLAKPKQPVDESMLMP
                     EIRTETLKPGTLIHEIKNPGTVTYLDKASVASRVLGRVQQIFVEQGSRVKKGEKLAQM
                     ETFEIILKQRQAQGSAAQARAQMLLAQARYSAARREVEKQLTGIERTQSNIVESRAQL
                     LTARQNLENKKELYDLGGVSGSELKQTYVTYLGAVSRLFQTRKEFQTQMIGFRDADLL
                     GANISVPEAPAEKKRTFIDFNTQVDLQSFRASEAAYQSAQIELENTNLLLREATIVSP
                     LDGVVAARSIEIGEEAKQNEPLFTVVRMDKLVVETQLPEEEIKSIAPGSKTAFTADAR
                     AGKVFEGELYIISPVIDPKTRSIPVRIRFDNNENLLAPGMFVRTRIRTREKKDALSIP
                     ETAVTNLRDQNSTRRGNVFVVKDGLTMKREIVLGDRFGERYEVTAGLSAGEVLAIDGI
                     DVLRDGARVKVKPELKAKVQEK"
     gene            complement(1800..>2439)
                     /locus_tag="PLG78_18185"
     CDS             complement(1800..>2439)
                     /locus_tag="PLG78_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25109.1"
                     /translation="LITSHDYLPSVSLTGHYGRTGEEWPPRSLDWGVGINVTIPLQGS
                     TLRTDAQYNSTKADTQRGLSEGGEFDFYNNPGYRAAGMRNELQLFRARANREDLRRNI
                     DLRIDKFARETRERNNELSLLAEKVSISELRNTVEYRKYISGQTALHDYLEEEIKLQQ
                     ARADLLEKRVESILAVGQFEADIGLELDYLGLIDLPENNPAPAPGQSAGITP"
BASE COUNT          507 a          598 c          628 g          706 t
ORIGIN      
        1 tgggagcaga tccgctcaga gtaaaagcat tctctgtttc attgggccgc atttctcggt
       61 cgaattccac gtaaacggga atgtcggaag gaacgttagg cgtcaaagca gaaggactgg
      121 aaagaacgac ggatggtcta gtcgcagaac ctggtgcgaa gagaatccct gggagattgg
      181 ctgcattctt gctgcaggaa ggaatgccag aaacagctaa aaacagggca aaaacaacac
      241 ctttcttttg aggaaggcca ctctggaagt aggggcagtt catttctcct gcacctttgc
      301 tttcagttca ggctttactt tgacgcgcgc accgtctctg agaacatcga ttccatcaat
      361 ggcaagtact tctcccgcgc tcaggccggc ggtgacttcg tatctttccc cgaatcgatc
      421 cccgagcaca atttcgcgct tcatcgtgag accgtctttc acaacgaata cattgcctct
      481 acgcgtgctg ttttgatctc gcaggttggt gactgcagtt tccggaatag acagagcatc
      541 tttcttttcg cgagtgcgga ttctggtgcg aacaaacata ccgggagcga ggagattttc
      601 attgttatca aagcgaatgc gcaccgggat gcttcttgtt ttaggatcaa tgacgggcga
      661 aatgatgtag agctcacctt cgaagacctt acctgcacgc gcgtctgctg tgaatgcagt
      721 ctttgatcca ggtgcaatac ttttgatttc ttcttccggg agctgggtct ccacgaccag
      781 tttgtccatt ctcaccacgg taaagagagg ttcgttttgt tttgcttctt ctccgatttc
      841 tatggatctg gctgccacaa cgccgtctaa cggggaaaca atggtagctt cgcgcagtag
      901 tagatttgtg ttttcgagct caatctgcgc actttgatag gccgcttctg acgctcgaaa
      961 gctctgcaag tcgacctgag tgttgaagtc tatgaatgtc cttttctttt ctgcaggcgc
     1021 ttccggaact gaaatgtttg cacccagcaa atctgcgtcc cgaaatccaa tcatttgagt
     1081 ctgaaattcc tttctggtct gaaagagtct ggatactgcg cccagatagg tgacatacgt
     1141 ttgcttgagt tctgagcctg agacaccgcc caggtcgtat agttccttct tgttttcgag
     1201 attctgtctg gcggtgagaa gttgggcgcg cgattctact atgttcgatt gagtacgttc
     1261 aattccagtc agctgctttt ctacctctcg gcgcgctgca ctgtagcgag cctgggccaa
     1321 aagcatctgc gctcttgcct gagctgcact tccctgggcc tgacgctgct tcagtatgat
     1381 ctcaaatgtt tccatctgag ccagtttttc tccttttttc acgcgagaac cttgttccac
     1441 gaagatctgc tgcacgcggc caagtactct cgatgcaaca gaagccttgt ccagatacgt
     1501 cactgtccct ggattcttga tttcgtgaat cagagtacct ggtttcagtg tttctgttct
     1561 gatttctggc atgagcatgg attcgtctac gggctgtttt ggttttgcca gctgatagat
     1621 agccatggtg attcccagaa cgctgcaggc gatgatcgca taacgtgcgc gcacgggcat
     1681 ggaccgcaat ctacgcattg ctatgcgacg atgaacctga aagcttgcag accggcctga
     1741 tcgtaagcgg tttagctttc gtcttatgaa tcgcatcgat gttttgaatt tgcgtgtttt
     1801 catggtgtga ttcctgcgga ttgaccggga gcgggagctg gattgttttc gggcagatca
     1861 atgagtccta agtagtccag ttcgagccct atatctgctt cgaattgacc gactgcgaga
     1921 atggattcca ctctcttctc gagcagatcc gcgcgtgctt gctgtaactt gatctcttct
     1981 tcgagatagt cgtgcaaggc cgtctgcccg ctgatgtatt ttctgtactc tactgtgttt
     2041 cgcagttctg aaatggaaac cttctctgcg agaagagaaa gttcattgtt tctttcgcgg
     2101 gtttctcggg cgaatttatc tatgcgaagg tcaatgtttc tccgcagatc ttccctgttt
     2161 gcgcgtgcgc gaaacagttg tagctcgttg cgcattcccg ctgctctgta tcctggattg
     2221 ttgtaaaaat cgaactctcc gccttcagac agaccgcgct gcgtgtctgc ctttgtgctg
     2281 ttgtattgtg cgtctgtgcg aagagtgctt ccctgtagcg ggatggttac gttgattccc
     2341 actccccagt ccagagatcg gggaggccac tcttcaccgg ttcttccgta gtggcctgtg
     2401 agagaaacag agggaaggta gtcatgcgaa gtgatcaga
//
LOCUS       DAOQJL010000577         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31767_length_2436_cov_5.529609, whole genome shotgun sequence.
ACCESSION   DAOQJL010000577 DAOQJL010000000
VERSION     DAOQJL010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31767_length_2436_cov_5.529609"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLG78_18190"
     CDS             <1..981
                     /locus_tag="PLG78_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE25110.1"
                     /translation="AYLLISYLGVNLAMTLLSVPHSAMAGELVPDPSERTALFGFRLL
                     FNCVGLLAGIISPSLSGSSGHGSIVIGVIALVSALITFRATRGLDVLPAKPESNVPRQ
                     PMLDVMRSTLSNRSFLLLVLAFLIASVGRTFNSSLALYYYKYRLLLSEEQTTRVVLGL
                     FVLVVTVSIVAWVLLARRYGKKRPAFAGILGLGLLTAIGYPLYPPGQLGGPILAAVLG
                     GFFVASIILFESLVADMAEAERVRSGEAREGIFFGFWKMANKAARALGLALSGVALSG
                     IGFSNTVTPGPEIAWKIALLFGPGVGACFIAAAVIFLGVQEPEKPVQNPV"
     gene            1039..2298
                     /locus_tag="PLG78_18195"
     CDS             1039..2298
                     /locus_tag="PLG78_18195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE25111.1"
                     /translation="MKRTLAIVGSGISGMGALYALRDEFAITLYEKADRIGGHTHTVT
                     VEDPSGPLPVDTGFIVYNTTTYPLLTRLFEELKIKTEWSDMSFGVQNLDTGLEWAGHN
                     LGSVFAQKKNLLRPAFWRMVLEILRFNRTALRRAQENSEEISLGDFLRNDRYSDYFID
                     NYIVPMGSAVWSTPARNMLDFPARTFIEFFSNHGFLEVERRVRWRTVSGGSHSYIKAI
                     LDEGKPEIQTGQPVTSVRRTASGKVEVTTPLEKREFDFVLMASHANETLSMLADPTHA
                     ETELLSHFHYQKNEAVLHFDESVLPSAPQCHAAWNFRTKSGVQAIAYSMKRLQNIKSD
                     RHYIVSLGEFERIDEKKIIRSMTYEHPLFDLKTAQAQKSLHLLNETGPIFFSGSYFRY
                     GFHEDGLWSASLAARRILDVSRQLVHV"
BASE COUNT          584 a          630 c          589 g          633 t
ORIGIN      
        1 gcatatctct taatttccta cctcggggtc aaccttgcca tgacccttct ctccgttcct
       61 cacagcgcga tggcggggga acttgttccg gatccatcgg aaaggactgc actatttgga
      121 tttcgccttt tgttcaattg cgtcggactg ctggctggaa tcatttctcc ttctctttcc
      181 ggatcttccg gccatggtag cattgtcatt ggagtgatcg cgcttgtctc tgcgctcatc
      241 acatttcgcg cgacgagagg tctggacgtc ctaccggcaa aaccagagtc gaatgtgccg
      301 cgccagccca tgctggacgt tatgcgatct acgctgagca accggtcatt tctcctcctg
      361 gttcttgcgt ttctgatcgc ctcagtgggt agaacattca actcctctct ggctctctac
      421 tactacaaat acagactctt actctcagag gaacaaacaa cacgcgtcgt cctgggactc
      481 tttgtacttg tggtcaccgt ttccattgtc gcatgggtcc tccttgccag aaggtacgga
      541 aagaaaaggc ccgcttttgc cggaatcctg ggccttggcc ttttgactgc cataggctat
      601 ccactttatc ctccgggtca gctgggtggt ccaattctag ccgctgtgct cggcggattc
      661 tttgtcgcat cgatcatcct tttcgagtct cttgtagcgg acatggcgga agcagagaga
      721 gtgcgttctg gtgaggccag agaagggatc tttttcggct tctggaaaat ggctaacaaa
      781 gccgcaagag cgcttggtct tgcactgtcc ggggtggctc tcagcggaat tggtttttca
      841 aacacagtaa ctcccgggcc agagattgcc tggaaaattg cacttctatt cggcccgggc
      901 gtcggagcct gttttatagc agcagctgtc attttccttg gtgtccagga gccagaaaaa
      961 cccgttcaaa atcctgtcta atgttttcaa aaaacattgg acgaaattca agaacaggat
     1021 ttccttatcc agtagcacat gaaacgcacg ctggctattg ttggttcggg gatctcggga
     1081 atgggagcgc tgtatgcgct gcgagatgaa ttcgccatca ccctgtatga aaaggcagat
     1141 cgaattggcg gacacactca tactgtgaca gtagaggatc cgtccggtcc gctacctgtg
     1201 gacactggct ttatagtata taacacaacc acgtaccccc tcctgactcg actcttcgaa
     1261 gaactgaaaa tcaaaacaga atggagcgat atgtcctttg gtgtgcagaa cctggacaca
     1321 ggacttgaat gggcgggtca caatctggga agcgtattcg cacagaagaa aaatctgctt
     1381 cggccggctt tctggagaat ggttctagaa attctcagat tcaaccgcac agcactcaga
     1441 cgcgcccagg aaaactccga ggaaatttct cttggcgatt tcctcagaaa tgaccgttac
     1501 agcgactact tcatagacaa ctacattgtc cccatgggct ctgctgtgtg gtccactccc
     1561 gcgagaaaca tgctggattt ccctgcgaga acattcatcg agtttttttc gaatcacgga
     1621 tttcttgaag tagaaagacg cgttcgctgg cgcactgtat caggtggatc gcattcctat
     1681 atcaaggcaa tcctggatga gggaaagcca gaaatccaaa cagggcaacc tgtaacatct
     1741 gtcaggcgca ccgcgagtgg gaaagtagaa gtaacaacgc cacttgaaaa gcgagagttt
     1801 gatttcgttc tcatggcgtc tcacgcgaac gaaactcttt ctatgctagc agatccaacg
     1861 catgcagaaa ctgagcttct gtctcacttt cactaccaaa aaaatgaagc agtgctgcat
     1921 tttgacgaat ccgtcttacc ttctgctccg cagtgtcatg cggcgtggaa tttccgcact
     1981 aaatctggag tgcaggccat cgcatattca atgaagcgcc tgcaaaatat caagtctgat
     2041 cgccactaca ttgtttccct gggagagttc gaacgcatag acgaaaagaa aatcattcgc
     2101 agcatgacat acgaacatcc tctattcgat ctgaaaacgg cccaggcaca aaaatccctg
     2161 cacctcttga atgagaccgg acccatattt ttctccggca gctattttcg ctacggcttc
     2221 catgaagatg ggctctggtc tgcgtccctc gccgcaagga ggattctcga tgtttcgagg
     2281 caactcgtgc atgtatgaag gacgtgtatc acacgtcaga cagaaacctg ttcgtaattc
     2341 atttacgtac ggtctctata tgttcgccat agacgtggat gaactcccca gactggatcg
     2401 gtccatgatt tcagtgaatc gtagaggcgt tttttc
//
LOCUS       DAOQJL010000578         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31793_length_2435_cov_4.300420, whole genome shotgun sequence.
ACCESSION   DAOQJL010000578 DAOQJL010000000
VERSION     DAOQJL010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31793_length_2435_cov_4.300420"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLG78_18200"
     CDS             complement(<1..371)
                     /locus_tag="PLG78_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNE25112.1"
                     /translation="MPRVLVPLADGFEEMEGIIIIDVLRRAGIEVVSASLKDGPITAA
                     RGTKHLADTTLDQALSQEFDMLVLPGGGPGAKALEADERVAQLLKEFHEKKKRIGAIC
                     AAPNALRRHNVIQGQDRFTMYP"
     gene            complement(378..1376)
                     /locus_tag="PLG78_18205"
     CDS             complement(378..1376)
                     /locus_tag="PLG78_18205"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNE25113.1"
                     /translation="MKHIAIVGATGAVGQEMIHVLEKRNFPVSKLTLLASKRSAGKTM
                     TFRGQAVVVQELTEQSFAGVDIALFSAGATMSKQFAPAAVKAGAVVVDNSSAFRMDPG
                     TPLVVPEINPEDIKLHKGVIANPNCCAIILLMAVYPIHKINPIKRIIVSTYQSASGAG
                     AKAMEELKKQAADVLAGRAPVPEVFQFPIAFNVFSHNAGMDLESGYNGEELKVIEECR
                     KILHSDIPISPTCIRVSTMRAHAESVHLELTSSADLDAIRNAISSSPGVRLQDDRKNN
                     HFPMPLEASGQDDVLVGRIRKDIGSTSGKEIDLFCCGDQLLKGAALNAVQIAEKLS"
     gene            1436..2218
                     /locus_tag="PLG78_18210"
     CDS             1436..2218
                     /locus_tag="PLG78_18210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE25114.1"
                     /translation="MKQVKRERAGLLWRLLPASARRKIAAYLTLSELQDLNESYRQYT
                     KQDPARRRTVQMELSSQIRTRRSIWPAAVSLTLTLVSVFLWIVQGKFGMSESLRLALF
                     GPLVMTAASPLSLYFLSPVRLRMLFRLPQTFESVAVCIVTMVFLIWLLYLIGESGGGV
                     LRVDRLTFIVLILGAMGAPLLEEVLFRELIPSMAPDILAGHALAVPIFAIAHFPDSPS
                     MFALYILAGGFLSMLRIQSGGLLLPLVAHAMANGIVTAARFG"
     gene            2255..>2435
                     /locus_tag="PLG78_18215"
     CDS             2255..>2435
                     /locus_tag="PLG78_18215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNE25115.1"
                     /translation="MAENLDIVVFGGSFDPPHAGHEQMARLALTRASRLIFMPAHRSP
                     LKHAQPARFEQRVRML"
BASE COUNT          493 a          629 c          636 g          677 t
ORIGIN      
        1 ggatacatgg taaagcgatc ctggccctga atcacgttgt gtctgcgaag tgcattgggc
       61 gcggcacaga tggcgccgat tctcttcttt ttttcgtgga attctttgag aagttgtgcc
      121 actctttcgt ccgcttccag ggctttggcg cctggtccgc ctccgggtag aaccagcatg
      181 tcgaattcct ggctcaaagc ctggtcgagc gtagtgtctg ccagatgctt tgttccgcgc
      241 gctgccgtga ttggtccgtc tttaagagaa gcggaaacta cctcaatgcc tgcacgtctg
      301 agcacatcaa tgatgatgat gccctccatt tcttcgaagc catctgcaag tggaacgaga
      361 actcttggca tagaacctca gctgagcttt tctgcgattt gcaccgcatt gagagcggcg
      421 cctttcagca gttgatctcc gcagcaaaaa agatcaatct ctttgccact tgtggatcct
      481 atgtctttac gaattctgcc caccaggaca tcgtcctggc cggatgcttc aagaggcatg
      541 gggaagtgat tgttctttct gtcgtcctgg agtctcactc caggggaaga agagatggca
      601 ttgcgaatcg catccaggtc cgcgctactt gtcagctcca ggtgaacaga ttccgcatga
      661 gcgcgcatgg tggacacgcg aatgcaagtg ggagaaatgg gaatgtctga atgcagaatt
      721 tttctgcact cttcgattac cttgagttct tctccgttgt acccgctttc caaatccatg
      781 cctgcgttgt gactgaagac gttaaacgct attggaaatt gaaaaacctc tggcactggc
      841 gcgcggcctg cgagcacgtc cgctgcctgc ttcttcagtt cttccattgc ttttgcgccc
      901 gccccggatg cggactggta tgtggataca atgatcctct tgataggatt gatcttgtgg
      961 attgggtaca ctgccatgag caagataatc gcacagcagt ttgggtttgc gatgacacct
     1021 ttgtgcagct tgatgtcttc cggattgatt tcaggtacga cgagtggagt tcccggatcc
     1081 atcctgaatg cggaagagtt gtctacgacc acggcccctg ctttgaccgc ggcaggcgcg
     1141 aactgcttgc tcatggtggc ccccgcgctg aacaaagcaa tgtctacgcc ggcgaaagat
     1201 tgttctgtga gttcctggac gaccacggcc tggccacgga atgtcattgt ctttccagcg
     1261 gaacgtttgc tggccagaag agtgagcttt gaaaccggaa aatttctctt ttcgagaacg
     1321 tggatcattt cctgacccac tgcacctgtt gcaccgacaa tagctatatg cttcattctt
     1381 tctctcctgg cacctgcacg cgcaaaacgg gcatccaatc ggattttttg ttcctatgaa
     1441 gcaggtcaag cgagaacgag cgggcttgct ctggcgcctg ttgccagctt ctgccaggcg
     1501 aaagatcgcg gcctatctga cactttccga gcttcaggat ctaaatgaat cctatcgtca
     1561 gtataccaaa caggaccctg cccgtcgacg aactgtgcaa atggaattga gcagtcagat
     1621 tcgcacccga agatccattt ggccggcggc tgtcagtctc acactcacct tagtctcagt
     1681 ttttctctgg attgttcagg ggaaatttgg aatgagcgaa tcccttcgtc tggctttgtt
     1741 tggaccgctt gtgatgacag cggcttctcc tttgagcctg tactttctat ccccggttcg
     1801 gcttcgcatg ctgtttcgac ttccgcaaac ctttgaaagt gtggctgtgt gcatagtaac
     1861 gatggtattt ttgatctggc ttttgtattt gatcggagaa agcggcggtg gagtactgcg
     1921 tgtagatcgc ctaacgttca ttgttttgat tctgggtgcc atgggtgcgc cgcttctgga
     1981 agaagttcta ttcagggaac tcattccttc tatggcgcct gacattctgg ccggccatgc
     2041 tctggccgtg ccgatattcg ccattgcaca ttttccagat tctccttcca tgttcgctct
     2101 ctatattctt gcaggcggtt ttctatcgat gcttcgaatt caaagcggtg gattgctttt
     2161 gcccctggtg gcccatgcta tggcgaatgg cattgtaacg gccgcgagat ttggttgatg
     2221 ttcctggtgc cacacttcaa tctgcaaaag aacaatggca gagaatcttg acatcgtagt
     2281 gttcggagga tcttttgatc caccgcatgc aggtcatgag caaatggcgc ggctggccct
     2341 tacgcgcgcg agtcgtttga tcttcatgcc tgcgcatcga tccccgctca aacatgcgca
     2401 acccgcgcgt tttgaacaac gtgtaaggat gcttg
//
LOCUS       DAOQJL010000579         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31794_length_2435_cov_3.970168, whole genome shotgun sequence.
ACCESSION   DAOQJL010000579 DAOQJL010000000
VERSION     DAOQJL010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31794_length_2435_cov_3.970168"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PLG78_18220"
     CDS             <1..121
                     /locus_tag="PLG78_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNE25116.1"
                     /translation="ENRHHIHESVFKALGRALRMAAELDDRATGIPSTKGMLE"
     gene            211..828
                     /gene="hisH"
                     /locus_tag="PLG78_18225"
     CDS             211..828
                     /gene="hisH"
                     /locus_tag="PLG78_18225"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNE25117.1"
                     /translation="MPGIAVLDYGMGNIHSILKALRLFHSDVTYTSDPARIRSSEALV
                     LPGDGAFEAAMEGLAPSRDLILQHVSEDKPLFGICIGFQVLFEDSSECESGIIPGLGL
                     LNGNIRRFQFQDPDIRVPHMGWNRLIPSSLADKEYYSDYMYFIHSYRAENTNPSEVVA
                     SCDYAGDTFPAVVRKDSILACQFHPEKSGDHGLFLIRDWVSSLSA"
     gene            833..1570
                     /gene="hisA"
                     /locus_tag="PLG78_18230"
     CDS             833..1570
                     /gene="hisA"
                     /locus_tag="PLG78_18230"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNE25118.1"
                     /translation="MFEIIPAIDLLDGQVVRLHKGDYDQKKVYSNDPPAFAREFVSAG
                     AGWIHVVDLNSARNGERSVNRSVLREIVQASGDARLQTGGGIRSSADIEEVLSLGISR
                     VILGTRAARSPGDIAGWISNFGADKIVIGVDVAGDRIRVQGWEEDGGYSTSDFLALLE
                     SAGVRLIVATNISSDGTLEGVDLEFYKRITDASRLEFVVSGGVAGIQDVVNLKQWNHP
                     QIHGMIVGKAYYEGRMDLVEAVRLAST"
     gene            complement(1589..2131)
                     /locus_tag="PLG78_18235"
     CDS             complement(1589..2131)
                     /locus_tag="PLG78_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25119.1"
                     /translation="MAKKAKKSGSGSAKSEAEKKKLLSAKNPDQYIQRSLDSKLSRGE
                     KGSLTREWLEKTGYTHEDILYARNRNPYWKKQKGRGSYERTLKRIKKLNFGGKSTGRK
                     VWSDADLGRFYELNDKMADWQLAQKFKTSLPAVNHIRRKFKLAKALLEKQRKTVNKGA
                     VVKLSRTDEKVLRTTLAGKR"
     gene            2246..>2435
                     /locus_tag="PLG78_18240"
     CDS             2246..>2435
                     /locus_tag="PLG78_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25120.1"
                     /translation="MFRHNVFLLIFAVSVLQCKESKAEVNPDMRKVVCLNSEQGCPCK
                     TDNGEFRIFHAEAARVTGP"
BASE COUNT          509 a          571 c          661 g          694 t
ORIGIN      
        1 cgaaaacagg catcacattc atgaatctgt gttcaaagcg ctcggccggg cacttcgcat
       61 ggctgcggaa ttagacgatc gggcgactgg aattccttct acaaaaggca tgctcgaata
      121 acatttttgt gttttttttg atccgcgcgc ggtgtgcacg attttgaaac gactttgacg
      181 cctggccccc tggcgggaaa ttcggtatgt atgcccggaa tcgcagtact ggattacggg
      241 atgggaaaca ttcactccat cctgaaggcc ctgcgtcttt ttcactccga tgttacctac
      301 acttcggatc cggcccgcat tcgttccagc gaagcactcg ttctgcctgg cgatggtgct
      361 tttgaagcag cgatggaagg acttgctcct tcgcgcgatc tcattctgca gcacgtttcg
      421 gaagacaaac cgttattcgg catttgtata ggttttcagg ttctgttcga agattcaagc
      481 gaatgcgaat cggggatcat tcccgggctt ggtcttttga acggaaacat ccgccgcttt
      541 cagtttcaag atccagacat tcgcgttccg cacatggggt ggaatcgtct cattccttct
      601 tctcttgcgg acaaagagta ctactcggac tatatgtact tcattcattc ttatcgtgcg
      661 gagaacacaa atccgtctga agtcgtggcc agctgtgatt atgcggggga tacgtttccc
      721 gctgtagtgc gaaaagattc tatccttgcc tgtcagtttc atcctgagaa atcaggcgat
      781 cacggacttt ttctaatccg ggactgggtt tcttctctga gcgcctaacg ttatgtttga
      841 aatcattcct gccattgatc tcctggacgg ccaggtggtt cgccttcaca agggcgacta
      901 cgatcagaaa aaagtctata gcaatgatcc gcctgcattt gcgagggaat ttgtttccgc
      961 aggcgccgga tggattcacg tcgtggatct caattcggcg cgcaacggcg agcgcagcgt
     1021 gaatagaagt gtactcaggg aaattgtgca agcttcagga gatgcacgtc ttcagacagg
     1081 cggcggcatt cgaagcagcg cagacattga agaagtcttg tctcttggaa tttcgcgagt
     1141 gattcttgga accagggcag ccaggtctcc cggtgacatt gcaggctgga tttcaaactt
     1201 tggcgcagat aaaattgtaa tcggcgtaga tgtagctgga gacaggattc gcgtgcaggg
     1261 ctgggaagaa gacggaggat attcgacgtc tgatttcctg gcgttgcttg agtcggcggg
     1321 tgtgaggctg attgtggcta cgaacatttc ctcagacgga accctggagg gcgtggatct
     1381 cgagttctat aaaaggatta cggatgcttc gcggctggag ttcgtggtgt cgggtggcgt
     1441 ggccgggata caggatgtag tgaatttgaa gcagtggaat catccgcaga ttcacggaat
     1501 gattgtaggt aaagcttact atgaaggccg gatggatctt gtcgaggccg ttcgactggc
     1561 ctcgacttaa tctaaagaat ctaaactatt aacgttttcc tgcgagagtg gtgcgaagaa
     1621 ccttctcatc tgtgcgactg agtttcacta ctgcaccttt gttcactgtc ttgcgctgtt
     1681 tctcgaggag agccttcgcg agcttgaact tgcggcgaat gtggtttacg gcaggaaggc
     1741 tggtcttgaa tttctgtgcc agttgccagt ctgccatctt gtcattgagt tcgtagaagc
     1801 ggcccagatc tgcgtcgctc caaactttgc gtccggtgct cttgccaccg aagttcagct
     1861 ttttgattcg cttcagtgtg cgctcgtagc tgccacgacc tttttgcttt ttccagtacg
     1921 ggttacgatt gcgtgcatac agaatgtctt cgtgagtgta tcctgttttc tccagccatt
     1981 cgcgcgtcag actgcccttt tcgccgcggc tgagtttaga atccagagat ctctggatgt
     2041 actgatcagg gttttttgcc gaaagtaatt tctttttttc tgcttctgat ttcgcagagc
     2101 ctgatcctga tttctttgcc ttttttgcca tactatgccg ccttgagatg gtcgcctaac
     2161 ggcgatattc acaggagttc tgtaaccgca ctcttcgtca accagttttg cttgcactgg
     2221 ggcaggcgct gtgcgatgat gcagtgtgtt tcgccataat gtgttcctgt tgatcttcgc
     2281 agtctctgtc ctgcaatgca aagaaagcaa ggcagaagtc aatcctgaca tgcgaaaggt
     2341 ggtttgcctg aattctgagc agggatgccc ctgtaagacg gataacggtg aatttcggat
     2401 ttttcatgct gaggcggctc gtgttaccgg gcctt
//
LOCUS       DAOQJL010000580         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31822_length_2434_cov_3.989491, whole genome shotgun sequence.
ACCESSION   DAOQJL010000580 DAOQJL010000000
VERSION     DAOQJL010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31822_length_2434_cov_3.989491"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /gene="argH"
                     /locus_tag="PLG78_18245"
     CDS             <1..1315
                     /gene="argH"
                     /locus_tag="PLG78_18245"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNE25121.1"
                     /translation="YREDLAGSRAHAKMLGKIGILTERELADIQRGLAQIEREIEEGK
                     FEIKHELEDIHTHIETRLTQIIGPSAGKLHTARSRNDQIAVDTHLYVRKSALLIGRQI
                     LDFCSALLDSAKKEIDTILPGYTHLQVAQPIRLSHHLLAHFWGFLRDASRFKTAYNTA
                     NRMPLGSGAMAGVNYKTDREFVQKELGFESLYENAMDAVSTRDHIQDFLYACASLGVR
                     ASRYAEEVVLWNSVEFGFIKITDGLTTGSSIMPQKKNPDTAELVRGRAGRSVANLMNL
                     LMNLKGLPFAYNRDLQEDRLPLLDSARSSMVVARALTALAGGIEFRRDRLTRSLSEGF
                     ALATDLADALVIEKGVPFREAHHIAGRLVGLCAERGFTLENAPESLRAEASDLLADES
                     FYKKAIDMTSSTDRKASRGGTARERQEEQIGIAQGELIRVRESLS"
     gene            1312..1899
                     /locus_tag="PLG78_18250"
     CDS             1312..1899
                     /locus_tag="PLG78_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25122.1"
                     /translation="MTPEAIKSYKAVFAECLTSIGEQVIPEIIQHLETIQDDAAAVRS
                     ARQDKKPGAPSHWITLLQFIKLGLVVSGELKAHYDELMGTYQTLTGRESRDENENKML
                     TEVGEFLYATDEATGDLDRINLRLMKSIRNEMIDQYGIVLHPNEAIYADRIPDENFER
                     LAPMIQELTGCCDRLLDLVRVMSALLRMQKFILSE"
     gene            complement(1867..>2434)
                     /locus_tag="PLG78_18255"
     CDS             complement(1867..>2434)
                     /locus_tag="PLG78_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25123.1"
                     /translation="RRNSRISSIPCIFLTGQTGSNSILQALRSGIDDYITKPFHSEEL
                     ADRIETIVRKKKPSVHEESLFVHFCDAHLLRSSSAAGDFDFYSSVFRRACQMISCATP
                     EVGLFFLSADTAQISPCSREKKVEARVVRVLAAASGMMQKRADVLIRRGDYLEPLAIR
                     GLRARYSAGRDRSIVEQWLLAQNELLHS"
BASE COUNT          687 a          623 c          618 g          506 t
ORIGIN      
        1 gtatcgcgaa gatctcgcag ggtccagagc tcatgcgaag atgctgggca aaattggaat
       61 cctgactgaa agggaactcg cggacattca aagaggtctc gcacaaattg agagagaaat
      121 cgaagaggga aaatttgaga tcaaacacga actcgaagat atccacacgc acatagaaac
      181 aaggctgact caaatcattg gcccttccgc cggaaaactc cacacagcaa gatcgcgaaa
      241 cgatcagatc gcagtggaca ctcatctgta cgtgcgaaag tccgctctcc tcattggccg
      301 ccagatcctg gatttctgct ctgccctcct tgattctgcg aagaaagaaa tcgatacgat
      361 tcttcccgga tacactcacc tccaggtcgc tcaacccatt cgtctgagcc atcacctgct
      421 ggcccatttc tggggatttt tgcgcgatgc aagcagattc aaaaccgcat acaacacagc
      481 aaacagaatg cctcttggat cgggggctat ggccggtgtg aactacaaaa cagatagaga
      541 attcgttcag aaagaactgg gatttgaatc tctctacgaa aatgcaatgg atgcagtctc
      601 cactcgagat cacatccagg attttcttta cgcatgcgcg tccctgggtg tgcgtgcatc
      661 cagatacgca gaagaagtag ttttgtggaa ttcagtagaa tttggtttca tcaaaatcac
      721 agacgggctg accacaggtt caagcatcat gccccagaaa aaaaatccgg acacagcaga
      781 gctggttcgc ggtcgcgctg gaaggtcggt tgccaatctc atgaatctgc tcatgaattt
      841 gaagggactt cctttcgcat acaatcgcga cctgcaagaa gacagacttc cacttctgga
      901 ttccgccaga tcatccatgg tagtggcccg cgcactgact gctcttgccg gaggaattga
      961 atttcgtcgt gatcgcctga cgcgttctct ttccgagggg ttcgctcttg ccacagatct
     1021 ggcagacgcg ctggtcattg aaaaaggcgt tccgttcaga gaagctcatc atatcgcagg
     1081 cagactcgtt ggattgtgcg cagagcgcgg attcactctt gaaaatgcac ctgaatcgct
     1141 acgagcggaa gcctcggact tactggcgga tgaatcattc tataaaaaag caatcgatat
     1201 gacttcgagc acggacagaa aagcaagcag aggcggaacc gcgcgcgaaa ggcaagaaga
     1261 acagattgga attgcacaag gagagctgat tcgcgttcgg gaaagtcttt catgacgccg
     1321 gaagcgatca aaagctataa ggcagtgttt gcagaatgcc tgacctccat tggagaacag
     1381 gtgatccccg aaatcatcca gcacctggaa accattcaag acgacgcggc agcagtacgc
     1441 agtgctcgcc aagacaagaa gccaggagca ccgtcgcact ggatcactct cctgcaattc
     1501 atcaaactcg ggctcgttgt ttctggagag ctcaaggcgc actatgacga actcatgggc
     1561 acataccaga ctctgaccgg gcgggaatca agagacgaaa acgaaaacaa gatgctcact
     1621 gaagtaggag aatttctcta cgcaacggat gaagcaacgg gagatctgga ccgcatcaac
     1681 ctgcgactga tgaaatcgat cagaaatgaa atgatcgatc agtacggaat cgttctgcat
     1741 ccaaacgagg ccatctacgc ggacaggatc ccggacgaaa actttgaaag actggcaccc
     1801 atgatccagg aattgactgg atgctgcgat agattgctgg atctggtcag agtgatgagc
     1861 gctcttctaa gaatgcagaa gttcattctg agcgagtaac cactgttcta cgatcgatcg
     1921 gtctctgcct gccgagtacc tggcacgaag tcctcgaatg gcaagcggct cgagataatc
     1981 gccacgacga atgagaacgt ctgcacgttt ctgcatcatc ccggacgcag cggcgagcac
     2041 acgcaccacc ctggcctcca ccttcttctc cctggagcaa ggagaaatct gcgccgtatc
     2101 tgcggacaaa aagaaaaggc cgacctcggg cgtggcgcag gaaatcatct ggcaggcgcg
     2161 acgaaacacg gaactgtaaa agtcaaaatc gcccgccgcg ctgctcgacc tgagtaaatg
     2221 cgcatcacag aagtggacaa acaaagactc ctcgtgcacg gacggtttct tcttccgaac
     2281 aatggtttca attctatccg caagctcttc tgaatgaaaa ggcttcgtaa tataatcatc
     2341 gattccgctt ctcagagcct ggaggatgga atttgatcca gtctggcccg tcagaaaaat
     2401 gcacggtata gaagagatgc gcgaattgcg tctg
//
LOCUS       DAOQJL010000581         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31936_length_2428_cov_6.190055, whole genome shotgun sequence.
ACCESSION   DAOQJL010000581 DAOQJL010000000
VERSION     DAOQJL010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31936_length_2428_cov_6.190055"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLG78_18260"
     CDS             <1..430
                     /locus_tag="PLG78_18260"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE25124.1"
                     /translation="MGHEMAHATAHHGAQRLFQDDIYQTAMNGAQQSIADLDPEKQRA
                     VMAALGAGAKFGVILPFSRDHETEADEMGILYMARAGYDPRESVKFWERMEKMSGGQP
                     PEFSSTHPSHGTRIARLKKFMPKAMEAYEKSDKQASNDSN"
     gene            417..905
                     /locus_tag="PLG78_18265"
     CDS             417..905
                     /locus_tag="PLG78_18265"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE25125.1"
                     /translation="MTPIDALIHQFEYGRMRLLKTLSDIEALPVADELLHWRMPFGKG
                     RAHIGWQMMHCAATLDRYLNVRILEKDPVDSHLVTNYAGGSNPDDQVRITPDQIRSAL
                     AATAPPFLSYFRALSEDRLSQKPHERADRTHVEIFHLLTWHEAHHQGQCHLAMNCFMA
                     GR"
     gene            949..2151
                     /locus_tag="PLG78_18270"
     CDS             949..2151
                     /locus_tag="PLG78_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25126.1"
                     /translation="MPSLLDSIQSGAYADARAQARELVSSDPRALSQSAVKKWAGMRF
                     RNLFISHATSDPAAVSAAVQKKLIGKDSRPDRQKISERFLRLDGPPEWQLEMPEAPAP
                     SLSNNVVLFCPGLLTGLLPVMAFQEAFPAVESKFGTRILQSDSHPMRGCEDNVADIEN
                     ALMKGIGLDAHAKKIVAGNEKIPGSAMAICYSKGMPDLLTALVRVPELRKKIRCIFNW
                     AGAPGGSPLADNIYNSVKDVDITVPEGLTGVLQVISPLLRIDEKVPRFSEYRVKEAAR
                     SLTTTERGAFLDRHLDEIDSWNIPVFNITGSTTVMEVPYFQMQGVMELNKYDANNDMQ
                     VTQNQAKLRTPMATDLAMMHGHHWDLSYSPFPPNMRFGAQHLDHPFPKESALVAMIQL
                     ALELGLVD"
     gene            complement(2155..>2428)
                     /locus_tag="PLG78_18275"
     CDS             complement(2155..>2428)
                     /locus_tag="PLG78_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNE25127.1"
                     /translation="ARVVKAFNTISANIMIQPKLEDGTADLFIAGNDEAAKKEVTAIA
                     QAWGWGSVNDMGDILMSFWLETFAMLWIYFGFKNNHWTHAFKLLRK"
BASE COUNT          610 a          614 c          623 g          581 t
ORIGIN      
        1 tatgggtcat gagatggcgc acgcaacagc acaccatggt gcgcaaagac tttttcagga
       61 tgacatttac cagactgcta tgaacggagc gcagcaatcc attgcggatc tggatccaga
      121 aaaacaacgt gctgtgatgg cggcactcgg agcgggcgcc aagttcggtg tgattcttcc
      181 ttttagccgc gatcatgaaa cggaagcaga cgaaatggga attctgtaca tggcaagggc
      241 agggtatgat ccacgcgaaa gcgtgaagtt ctgggaacgc atggagaaga tgagcggcgg
      301 tcaacctccg gaattttctt ctactcatcc atcgcacggg actcgcatcg cgcgtctgaa
      361 gaagttcatg ccaaaagcaa tggaagcata cgaaaagtca gacaaacagg cgagcaatga
      421 ctccaattga tgcgttgatc catcagttcg aatatggaag aatgcgtctg ctcaagacgt
      481 taagcgatat tgaggcattg cccgtggcgg acgaactcct gcactggcgg atgccttttg
      541 ggaagggacg ggctcacatt ggctggcaga tgatgcactg cgcggccact ctcgatcgtt
      601 acttgaatgt tcgcattctg gaaaaagatc cggtagattc gcacctggtc acaaactatg
      661 cgggtggaag caatccggat gatcaagtgc gcattactcc ggatcaaatc agatctgcac
      721 tggcagcaac agctccacct tttctatctt actttcgcgc cctttcggag gacaggctat
      781 ctcagaagcc gcacgaaaga gcggacagaa ctcatgtgga aatatttcat ttgcttacgt
      841 ggcatgaggc gcatcaccag gggcagtgcc atctggccat gaactgcttc atggcaggtc
      901 gataacggaa aatgcttgtc tccggacgga aatgccttac agtccggtat gccatcactc
      961 ctcgattcca ttcagtccgg tgcgtacgca gatgcgcggg cccaggcccg cgaactcgtt
     1021 tcgtctgatc ctcgggctct ctctcagtca gccgtcaaga aatgggcggg gatgcgattt
     1081 cgcaatttat tcatatcgca tgcaacatcc gatcctgcag cagtctctgc ggccgtacaa
     1141 aagaagttaa tcggaaaaga ctcccggcca gacagacaga agatctccga gcgctttctg
     1201 cgcctcgatg gacctcctga atggcaactt gaaatgccag aagcaccggc accctcattg
     1261 tcgaataacg ttgtactttt ttgtccgggg cttcttacag gccttcttcc tgtgatggct
     1321 tttcaagagg cgtttcccgc tgttgaatca aaattcggaa ctcgcattct ccagagcgat
     1381 tctcatccaa tgcgcggatg cgaagacaat gtggcggaca ttgaaaacgc tttgatgaaa
     1441 ggcattggtc tggatgcgca cgcaaaaaaa atcgtggcag gaaatgagaa aattccaggc
     1501 tccgcgatgg ccatttgcta ttccaaaggc atgccggacc tactcaccgc tcttgttcgc
     1561 gtccctgaac tgagaaagaa gattcgttgt atttttaact gggccggcgc tccgggcggt
     1621 tcgcctctgg cagacaatat ttacaattca gtgaaggacg tggacatcac agtgccggaa
     1681 ggactgacag gcgtattgca ggtgatttct cctcttcttc gtatcgatga aaaggtaccg
     1741 cgatttagcg agtacagagt gaaagaagcc gcgagatccc tcaccacaac ggaacgcgga
     1801 gcatttctcg atcgtcacct ggatgaaatt gattcctgga acattcctgt tttcaatatt
     1861 acaggatcta caactgttat ggaagttccg tattttcaaa tgcagggcgt gatggagcta
     1921 aacaaatatg acgcgaacaa cgacatgcag gtgacccaaa accaggcaaa gctcagaaca
     1981 cccatggcaa cagacctggc catgatgcac gggcaccact gggatctgtc ttatagtccg
     2041 tttccgccta acatgcggtt tggtgcacaa catctggatc atccattccc caaggaatca
     2101 gcccttgtgg cgatgatcca gcttgcgctt gagcttggtc tcgtagattg atttctactt
     2161 tcgtagaagc ttgaaagcgt gcgtccagtg attgttcttg aatccgaaat agatccagag
     2221 catcgcgaac gtctcgagcc agaatgacat cagaatatct cccatgtcat ttacgctgcc
     2281 ccagccccag gcttgtgcaa ttgcagttac ttctttcttg gccgcttcat cattgccggc
     2341 gataaacaaa tcggctgttc catcttcgag ctttggctgg atcatgatgt ttgcgctgat
     2401 cgtgttgaag gctttcacta cacgcgct
//
LOCUS       DAOQJL010000582         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_31939_length_2428_cov_4.808681, whole genome shotgun sequence.
ACCESSION   DAOQJL010000582 DAOQJL010000000
VERSION     DAOQJL010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31939_length_2428_cov_4.808681"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /gene="trpS"
                     /locus_tag="PLG78_18280"
     CDS             <1..861
                     /gene="trpS"
                     /locus_tag="PLG78_18280"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNE25128.1"
                     /translation="VQEGPALREGIHQAAVDFLALGLDPDKCTFWVQSDVHEVTELAW
                     LLSTSITVSQLELAHSYKDKIAQGIIPSAGLFTYPVLMAADILLFGSNRVPVGKDQKQ
                     HLEITRDICHRFNTRYGEVFVMPEPDILDETALVPGVDGRKMSKSYGNAIYFFADEKE
                     LKKAVMSIVTDSQGVHDPKDPDKSVLYQIYSLFLDEPGKAALADRFRTPGTGYGDLKK
                     ELLARVLDTFQPYRKRREELLASPEEVSALMRKGAEKARAAAMPIISKAREAVGIAPK
                     TATLPKQKKK"
     gene            897..>2428
                     /locus_tag="PLG78_18285"
     CDS             897..>2428
                     /locus_tag="PLG78_18285"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNE25129.1"
                     /translation="MGRSATVSKAILVLFALCVLLLRSSFDSLATDLYAHAYAWQVAH
                     SDVVPGPRPLPEFAWVFAICTLPLVILLPERIRSWAVASVLFASFCIRLAGIPALPSL
                     PSYSRDPSAAHSNFRREALRVTMVVKESVSSSSHVAEFRITGREVKVRRQWFSISDLA
                     EEEKSAFPVLVHFEGARLYGGCKVHARISLRAMPREIKNESYASYVHRLGAVGTLRLE
                     PWQVLAIECGSPGWRDSVTLFIERSLGHRFRGRTLDASLGFVLGQAGTLDSELKTSAR
                     ELGILHVFAASGMHLAIIYGVIFLPASFLLGRRHPISALLPIGPCFLYLWLLGFPVSL
                     ARAFAFAVVYALTCLFHRRLTSTTLLLNSAILLLFWMPEDFATLSSALSFGAVAGIFY
                     FASPLLTLFRLKGPSAWIWGQVSVSACAGIFTTPLLIFVFGGFSFSSLPANLVAVPLS
                     DLALPVLFGGLGLESMGAPEQVLEIAWKPAQWIMSAFIFLTERAGPHSLYNDFDSIWS
                     LPL"
BASE COUNT          485 a          589 c          657 g          697 t
ORIGIN      
        1 gtgcaggaag gtcctgcttt gagggaaggg attcatcagg cagctgtgga tttcctggca
       61 ttgggtcttg atccggacaa atgcactttc tgggttcaaa gcgatgtgca tgaagtaaca
      121 gagcttgcgt ggcttctttc cacgagcatc acagtttcgc aactggagct tgctcattct
      181 tacaaagata aaattgcgca gggcatcatt ccttccgctg gcttgtttac gtatcccgtt
      241 cttatggcgg cagacatact gttatttgga tccaaccggg tgcctgttgg aaaagaccag
      301 aagcaacatc tggaaatcac gcgtgatatc tgtcatcgtt tcaataccag atacggtgaa
      361 gttttcgtga tgccagagcc agacatcctg gatgagactg ctctggttcc gggtgtagac
      421 ggtcgcaaga tgagcaagag ctacggcaat gcgatttact tcttcgcaga cgaaaaggaa
      481 ctcaagaaag cagtcatgtc gattgtgacg gattctcagg gcgtacatga tccaaaggat
      541 ccggacaaat ctgttctcta tcagatctat tctttgtttc tggatgaacc gggaaaggcg
      601 gctcttgcag atcgatttcg cacacccggc actggctatg gcgatttgaa aaaggaactg
      661 cttgcgcgcg tgctcgatac gtttcagcca tatcgcaagc ggcgcgaaga gctcctggct
      721 tcccccgaag aagtgtccgc attgatgcga aaaggggcag agaaagcaag agccgcggcc
      781 atgcccatca tttcgaaggc aagggaagca gttggaattg caccgaagac tgccactctg
      841 ccaaagcaaa agaagaagta agcggaacga atgaaactgc ccgcacactc atctgtgtgg
      901 gcagatctgc aaccgtttca aaagcaattc tggttctatt cgcactctgt gttttgcttt
      961 tgcgctcttc ctttgattcg ctggcaacgg atctctatgc gcatgcatat gcatggcagg
     1021 tagcgcattc agacgttgtt ccaggtcccc ggccattgcc agagtttgcc tgggtatttg
     1081 cgatctgcac actgccttta gtcattttgc ttccggaaag gattcggtcc tgggcggtcg
     1141 cctctgtttt gtttgcttct ttctgtattc gtctggcagg aattccggct cttccgtctc
     1201 tgccttccta tagcagagat ccttcagcag cgcattccaa tttcaggaga gaggctttgc
     1261 gcgtcaccat ggttgtgaaa gaaagcgttt cttcttcttc gcatgtggca gaatttcgca
     1321 taacaggccg tgaggtgaaa gtgagacgtc agtggttttc catatcagat ctggcagaag
     1381 aggagaaaag tgcgtttcca gttcttgttc attttgaagg agcaaggctc tacgggggat
     1441 gcaaggtgca cgctcgcatc tcgctccgtg ctatgccccg tgaaatcaaa aatgaatcgt
     1501 acgcatcgta cgtgcatcga ctgggcgcag tgggcacctt gcgtttggaa ccctggcagg
     1561 tgctggccat agagtgtgga tctccgggat ggcgcgattc tgtgacgctt ttcattgaaa
     1621 ggtctttagg tcatcgcttt cgcgggcgaa cgcttgacgc ttccctggga tttgtgcttg
     1681 gccaggcggg gactctggat tctgaactca aaacaagtgc gcgtgaactc gggattctgc
     1741 acgtgtttgc ggcaagtggc atgcatctgg cgatcatcta cggagtgatc tttctgccgg
     1801 cctcgttcct tcttggtcgc aggcatccca tttctgcgct tcttccgatt ggtccatgtt
     1861 tcctctattt gtggctgctc gggtttcctg tgtctcttgc aagagccttc gcattcgcag
     1921 tcgtatacgc tctcacctgt ctgtttcatc gtcgcttaac gtctactact ctgcttttga
     1981 attcggccat attactcctg ttctggatgc ctgaggattt tgcgactctg tctagcgctc
     2041 tgtcttttgg tgcggtggcc ggtatctttt actttgcttc gccgcttcta acgctctttc
     2101 gactcaaagg gccctctgcg tggatctggg gccaggtgtc tgtgagtgca tgcgctggaa
     2161 tcttcacgac tcctcttttg atctttgtgt ttggcgggtt ttcattttct tctttgccgg
     2221 ccaatctagt ggccgtgcca ttgagcgatc ttgctttgcc tgttctgttt ggaggcctgg
     2281 gtcttgagag catgggcgca ccagagcagg tgctggagat tgcgtggaag ccggctcagt
     2341 ggatcatgag tgcttttatt tttttaacgg agagggcagg gccgcattct ttgtacaatg
     2401 actttgattc catctggtct ctgccact
//
LOCUS       DAOQJL010000583         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_32025_length_2424_cov_4.829464, whole genome shotgun sequence.
ACCESSION   DAOQJL010000583 DAOQJL010000000
VERSION     DAOQJL010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32025_length_2424_cov_4.829464"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PLG78_18290"
     CDS             <1..1667
                     /locus_tag="PLG78_18290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE25130.1"
                     /translation="EVGFSYRGYREFMHHNASRAVILLLAALLFVLIGFRVFFSGALL
                     GPLEALLRGVRKVNAGNLDVAVTARVEDEIGFLTHSFNGMVSSIRDARLKLQDYANSL
                     EEKVKARTAELQETLTQVQSLKSQQDGDYFLTALLLRPLRANRAKSDAVSVEFFQRQK
                     KHFTFRHWQEEIGGDLCMSNNLRLNGRPFTVVMNADAMGKSMQGAGGALVLGSVFEAI
                     LERTQLSAAMQEQFPERWIKNAFIELHKVFEAFECSMMVSLVIGLIDEESGLLYYINA
                     EHPFSILYRRGKAAFMDVETLFRKLGSPATSGNISIRTFQLQPGDVMIIGSDGRDDIL
                     LSEEGREPFMNEDESKILGHVEASDGNLQGIFDRLTSWGTIIDDLTFIRIGYRESGGV
                     PEALIESPVNKEIRDLLVKSGELTRAQNFAGAVPLLEQAFKLDDRHPEVIKRLALAYV
                     NLKMFDKAAPLAEDYTYLRPGDSEFVYVASYCLKKVKEYSRAADYGERLKLRNPRHVK
                     NLINLAEVYAKMRNLARAELLLDSALLVDPDNTTARSRLEKVRRAA"
     gene            1744..>2424
                     /locus_tag="PLG78_18295"
     CDS             1744..>2424
                     /locus_tag="PLG78_18295"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE25131.1"
                     /translation="MQGPEIPGFATGPVLLAHRGIVWHRASREGRDFLLKTANLSSAE
                     SSTLLQREFNLLRTKSIAGIPKPLEFLRMGHQATIVFDGEAGEVLSRVLAREKAMEIL
                     RLLDVAVAIVEVVQSFHANGITHRSLTEERILLHADGRVVVLDFHNATTFVQERAPAL
                     MAAADMDMHYLAPEQTGRTNLLVDFRADLYALGALFYKMLTGRLPFASADSLELVHAQ
                     LASMPAPPS"
BASE COUNT          576 a          560 c          644 g          644 t
ORIGIN      
        1 cggaagtagg gttttcgtat cgcggataca gagagttcat gcatcacaat gcttcacgcg
       61 ccgtgatttt gttgctggca gcgctccttt tcgttctcat tggatttcga gtgttctttt
      121 ccggcgcact tcttggtcca ctcgaagcac ttcttcgagg cgttagaaaa gtaaacgctg
      181 gcaatctgga tgtcgcggtc acggcgcgag tagaggatga aattggcttt ttgacgcaca
      241 gctttaatgg aatggtttct tcgattcgcg acgctcgtct caagttgcag gattatgcga
      301 attccctgga agagaaggtg aaggcccgaa cagcagaatt gcaggaaaca ttgacccagg
      361 tgcaaagcct gaagtcgcaa caggatggag attacttttt aacggccttg ctccttcgtc
      421 ctctgcgtgc gaatcgcgcg aagagcgatg ctgtatccgt tgagtttttt cagaggcaga
      481 agaagcattt cacattccgt cactggcagg aagaaatagg cggagacctt tgtatgtcca
      541 acaatcttcg tctgaacgga aggcccttta cggttgtgat gaatgcagat gcaatgggta
      601 aatccatgca gggagcgggc ggcgctctgg ttcttggctc agtctttgaa gcgatcctgg
      661 agagaacgca gctaagtgcg gccatgcagg aacaattccc ggaaagatgg atcaagaacg
      721 catttataga acttcacaaa gtattcgaag cattcgaatg ctctatgatg gtttcgcttg
      781 tgattggttt gatagatgaa gaatctggtc ttctttacta tataaatgca gaacatccat
      841 tttctatact atatagaaga ggtaaggctg cattcatgga tgtggagact ttgtttcgca
      901 aacttggttc tcctgctact tcgggcaaca tttccattcg cacgtttcag ttgcagcctg
      961 gagatgtgat gatcataggg tctgacggtc gcgatgacat tcttctttcg gaagagggcc
     1021 gcgaaccgtt catgaatgaa gatgaaagca agatcctcgg gcatgtggaa gcgtcagacg
     1081 gaaatctgca gggtatcttt gatcgtctca cgtcatgggg cactatcata gacgatttga
     1141 cttttatccg aattggatac agggaaagtg gcggagtgcc tgaggcgttg attgaatccc
     1201 ccgtcaacaa ggagattcgc gacttactgg ttaagtctgg cgagctcact cgagctcaaa
     1261 atttcgcggg cgcggttcct ctccttgaac aagcatttaa acttgacgac agacatcccg
     1321 aagtcatcaa acggctcgcg cttgcgtacg tgaatttgaa aatgttcgat aaggcggcac
     1381 cgcttgcaga agactataca tacttacggc caggcgattc ggaatttgta tacgtcgcgt
     1441 cgtattgctt gaagaaagtg aaggaatatt cgcgcgctgc agactacggc gaacgcctga
     1501 agcttcgcaa tccaagacat gtgaagaatc tgatcaatct ggcagaagtg tacgcgaaga
     1561 tgagaaacct cgctcgcgcg gagctgcttc tggattctgc gctccttgtg gatcctgaca
     1621 atactactgc tcgtagcagg ctagaaaaag tcagaagggc tgcttgaaat agcgatccga
     1681 acttttccgg attctcggat ttagtttgcc attttcagcc acctgcgcac gagttattcg
     1741 gatatgcaag gtccagaaat tcctggcttc gccactggtc cagtcttgct ggctcatcgt
     1801 ggaattgtct ggcatcgcgc aagtagagaa ggacgtgatt tccttctaaa aaccgcgaac
     1861 ctcagttcag cggaaagttc cactcttctg cagcgtgaat tcaatcttct ccgtacgaaa
     1921 tcaattgcag gcattccgaa gcctcttgag tttttgcgta tgggtcacca ggcgacgatc
     1981 gttttcgatg gagaggctgg tgaggttttg tccagggttc ttgcgcgcga aaaagcaatg
     2041 gagattttgc gacttctcga tgtggctgtg gcgattgtag aagttgtcca atcatttcat
     2101 gcgaatggga tcacgcaccg gagcctgacg gaagagcgga tccttttgca tgcagatggt
     2161 cgcgttgttg tccttgattt tcataacgcc accacctttg tccaggagag agccccggcg
     2221 ctgatggcgg cggcagatat ggacatgcac tatcttgctc cagaacagac tggacgcacg
     2281 aatcttctcg ttgatttcag agcggatctt tacgctctcg gcgcgctctt ctacaagatg
     2341 ctcaccggta gacttccgtt cgcaagcgca gattctttgg aactcgttca tgcgcagctc
     2401 gcttccatgc ctgctcctcc ctcg
//
LOCUS       DAOQJL010000584         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_32052_length_2423_cov_4.724240, whole genome shotgun sequence.
ACCESSION   DAOQJL010000584 DAOQJL010000000
VERSION     DAOQJL010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32052_length_2423_cov_4.724240"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLG78_18300"
     CDS             <1..742
                     /locus_tag="PLG78_18300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNE25132.1"
                     /translation="GEEERLEKTTYLLSKGAKLDVKTKEGFTPLHIAVQSGDEIAMLL
                     IEKGANIQAVSNTGATMLQLAAGAGSARLVKYFLDHGANVQAKNGEGYTAIFYGAQSG
                     SGEVVKLLLDKGAVCTDYGKDRKTPLFLIAMFPTAEAHYETLEENPQDFYTALTLVLQ
                     KSGPALIHSPDIYGETPWFLLKGWMYPLPEKIRLFLKYGANKNARNPSGQTLLDVLQN
                     ELRDEESSLADGARENIEESIRVLKNGP"
     gene            732..1631
                     /locus_tag="PLG78_18305"
     CDS             732..1631
                     /locus_tag="PLG78_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25133.1"
                     /translation="MGRNASPWSVNLNELSFDAGILFCTAIIRFALFGDRPIAEALAS
                     WQLVVVYLLVCFAIPSYMGYVLGRYAKFTGRFTQRLILTIFSVVILTIFVFLTRIMLQ
                     LQDQNLMGFLLCSGTGLLLMGSIAGIVTMANAEDTEVVTNAFENPILSILGVTGSVIL
                     LFYLGIYKPFGITFEGGLGFLEFLAVFVGCPFAGVLFILVVGLAENAVMKAGGGEVLE
                     RMRHVLIPLFIAVSLMIFSNFAGHFLGQTSQSVGGSSLARILLFTFSGVLPFRIVLVF
                     MPPVRITNVIIGSVVLTASFISA"
     gene            complement(1661..>2423)
                     /locus_tag="PLG78_18310"
     CDS             complement(1661..>2423)
                     /locus_tag="PLG78_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25134.1"
                     /translation="NATSVLEIDPIARTAVQFGTVSGATGKWEGGVLASNGKIYAMPS
                     SVTTVLEIDPINRVVNQNAAVTGGSFYEIGSVLGPNGKVYGIPYSNAGPAQLMELDPA
                     TGSTVLFGPSFPAFSFTSGVLAPNGMIYTIPYTNTAIYEIDPTKRTATAVTGVLVGGY
                     SNGSLAPNGKIYMFPYDAPNTNVLEFDPNTKQMTSIGNNFAGGLANKYEDGVMAPNGK
                     IYTIPRDATNVAEISTGSVGTFCTGLLTSSYLNHY"
BASE COUNT          563 a          557 c          628 g          675 t
ORIGIN      
        1 tggagaagag gaacgtcttg aaaagacaac gtatcttctg tcaaagggcg cgaagctgga
       61 tgtgaaaacg aaagaaggat tcacgcccct tcacatcgcc gtgcagtctg gtgatgaaat
      121 cgcaatgttg ctaattgaaa aaggtgccaa catccaggca gtgtcgaata cgggcgccac
      181 catgttgcag cttgccgcgg gcgcaggatc cgccaggctc gtgaaatatt ttctggatca
      241 cggagcaaac gtccaggcaa agaacgggga agggtacact gccatttttt atggggctca
      301 aagcgggagc ggagaggtag tgaagcttct tcttgataag ggcgctgtgt gcacggatta
      361 cgggaaggat agaaagactc cgctctttct catcgcaatg tttccaacag cagaagcgca
      421 ctatgaaact ctggaagaga atccgcagga tttttatacg gcactcaccc tggtgttgca
      481 gaaaagcggt ccggcgctta ttcattctcc cgacatttac ggcgaaaccc catggtttct
      541 tttgaaagga tggatgtatc cactgcctga aaaaattcgg ctgtttctca aatatggagc
      601 aaacaaaaat gcgcgcaacc cgagcggtca aaccctgctc gatgtgctgc agaatgagct
      661 tagagacgaa gaatcgagtc tggcagacgg cgcacgtgag aacatagagg aaagcattcg
      721 agtgctcaag aatgggccgt aacgcttcgc catggtccgt gaacctgaac gagctttcgt
      781 ttgatgctgg aatcttgttc tgcactgcta tcatacgatt tgcactcttc ggagatcgac
      841 cgattgctga ggccctggcg tcgtggcagc ttgttgttgt ttacctgttg gtttgttttg
      901 ctataccgtc ttacatgggt tatgtgctcg gtcgatatgc aaaatttaca ggcaggttta
      961 cgcagagact gatcctcaca atattttctg tcgttattct gactattttc gtttttctta
     1021 cgcgaatcat gttgcagctt caagatcaaa atctgatggg attcctgctc tgttccggga
     1081 ctgggctgtt gctgatgggt tcgatcgccg ggatcgtcac gatggcaaat gcagaagata
     1141 cggaagtagt caccaatgcg tttgaaaacc ccatactttc catactcggc gtgactggtt
     1201 ctgtgattct tcttttctat ctgggtattt acaaaccctt cgggattact ttcgaaggag
     1261 ggttggggtt tctagaattt ctggctgtgt tcgttggctg ccctttcgcg ggcgttctct
     1321 tcattctagt agtcgggctt gctgaaaacg cggtcatgaa agcgggcggc ggggaggtgc
     1381 ttgaacggat gcgccatgtg ctcattcctc tttttattgc agtttcactt atgatcttca
     1441 gtaacttcgc aggtcacttc ctgggacaga ccagtcaatc agtcggagga tcatcgcttg
     1501 cacgcatttt acttttcacc ttttcggggg tccttccctt tcggatcgta cttgttttca
     1561 tgcctcctgt tcgaatcaca aacgtgatca ttggcagtgt ggttttgacg gcatccttta
     1621 tcagtgcgta aggatttcaa gaaaccagga cgttaggcga ctaatagtgg ttcaagtagg
     1681 aagaagtaag caatccggtg caaaacgtgc cgactgaccc tgtcgaaatt tctgcaacat
     1741 tggttgcgtc tcgcggaatg gtgtagatct tcccattcgg tgccatcaca ccatcttcat
     1801 atttgtttgc caggccgcct gcgaagttat ttccaattga agtcatttgc tttgtgttgg
     1861 gatcaaactc aagtacgttt gtattgggtg catcgtaagg gaacatgtag attttcccgt
     1921 taggcgccag cgaaccattc gagtaccctc ccaccagaac cccggtcact gcggttgcag
     1981 ttctttttgt cggatcaatt tcgtatatcg ctgtgtttgt gtatggaatg gtatagatca
     2041 ttccgttagg tgcaagcact ccacttgtga aagaaaatgc tggaaacgaa ggcccgaaca
     2101 gaacagtgga tccagtagca ggatcgagtt ccatcaattg cgcgggccct gcgtttgaat
     2161 atggaattcc gtacactttc ccgtttggcc cgagcacgga acctatttca taaaagcttc
     2221 cgcctgtgac ggctgcattc tgattcacca cgcggttgat tggatcgatt tcgagtactg
     2281 tggttacaga ggaaggcatc gcatagatct ttccattgga tgcaagaact cctccttccc
     2341 attttcctgt ggctccagat acagtgccga attgaacggc agtgcgcgcg attggatcaa
     2401 tttccagaac gctcgtcgcg ttg
//
LOCUS       DAOQJL010000585         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_32554_length_2403_cov_4.220187, whole genome shotgun sequence.
ACCESSION   DAOQJL010000585 DAOQJL010000000
VERSION     DAOQJL010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32554_length_2403_cov_4.220187"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="PLG78_18315"
     CDS             complement(<1..1681)
                     /locus_tag="PLG78_18315"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE25135.1"
                     /translation="MVRRVLVISLTIFALAGRVQADPVSTEERPIILTSDLRHRQLGR
                     EVDVLEDASGQLTIHDVKSGSARLRFRPGSSDVLSFGFTHSTYWIRARLENRSNENLW
                     FLQVAFPLIDHIELFEEERLVSQTGTQFPFSTRDMEHRTFVYKIKPASTTYYLRVQNE
                     DSMQIPLAVWSPEAFHSADHDEQMALGAYYGILIVMAAYNLFLFLSLRERGYFYYVIY
                     ITVFGFFLFSQYGFAYEYLWPNWTTLARKMNPVLAGALEASTLIFTRHFLNTRAHAPR
                     LDQVITVLIYLACLASPLALFLNLTHSAVMVVCLGLAASKCALVAGSLSLRAGFRPAR
                     YYMTAFLLLIVGAVLYALKTFGAIPVTFVSQYGMQLGSALEVTLLSLGLADRMNIMRR
                     QTETAQRQLLEEKSHSLERQTDLTRAYARMVPGEFLGILGRNSILEVKLGDSVQKTMT
                     VLFSDIRSFTELSETMTPRENFDFLNSYLRRMNPIIQRNGGSIDKYIGDAIMALFPQS
                     PEGAVRAAVEMQKALHEFNLHREERGFRPISIGIGIHTGNLMFGTIGGDERM"
     gene            complement(1972..>2403)
                     /locus_tag="PLG78_18320"
     CDS             complement(1972..>2403)
                     /locus_tag="PLG78_18320"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNE25136.1"
                     /translation="RTGRRCVNPKILSLSHGNSRIAYMNDNMLFLALGFFAGTISGLI
                     GIGGGIILVPVLVFFFAFSQQQAEGTTLAAMVPPIGALAAYVYYRAGFVNVPAAMWIA
                     GGFIVGGFVGARFATSINPEMLRRIFGVFAVAIGAKMLWGA"
BASE COUNT          683 a          623 c          588 g          509 t
ORIGIN      
        1 ccatcctctc atcgccgcct atcgttccga acatcaggtt tcctgtgtga atgccaatcc
       61 cgatgctgat cggtcgaaaa cctctttcct ccctgtgaag gttgaactca tgcagggcct
      121 tctgcatttc taccgcggcg cgaaccgcac cttccgggga ctgcggaaac aaagccatga
      181 tcgcatcccc gatgtattta tcgatggacc caccgttgcg ttgaatgatg ggattcatgc
      241 gccggagata ggaattcagg aaatcaaaat tttcgcgcgg cgtcatggtc tcagaaagtt
      301 cagtgaaaga tcgaatatca gaaaatagaa ccgtcatcgt tttttgaacg gaatcgccca
      361 gcttcacttc cagaattgaa ttgcgaccga gtatacccag aaactccccg ggcaccattc
      421 gcgcgtacgc gcgagtcaaa tcggtctgcc tctccagcga atgcgatttc tcttccagca
      481 actgcctctg tgcagtctcc gtctggcgcc gcataatatt cattctgtct gccagaccga
      541 gcgaaagaag agtcacttcc agtgccgaac ccagctgcat tccatactgc gaaacaaacg
      601 tcactggaat ggccccgaat gtttttagcg cgtaaagtac cgcgccaacg atgagcaaca
      661 aaaaagcagt catgtaatac cgcgcaggac gaaatccggc gcgcaaactc aagctacccg
      721 cgaccagagc acacttgctt gcagcaagac caagacaaac gaccatcacg gccgaatgag
      781 tcagattcag aaacaacgcc aggggagacg caagacacgc caaatagatc aagaccgtaa
      841 tgacctggtc gaggcgcgga gcgtgagccc gagtgttcaa gaaatgacgg gtgaaaatca
      901 gagtgctggc ttccagagca ccggcgagga ctggattcat tttgcgtgcg agagttgtcc
      961 agttcggcca gagatattcg tatgcaaagc catactgcga aaacaagaag aatccaaaga
     1021 ctgtaatgta aataacgtag tagaagtatc ctctctcccg gagcgaaaga aatagaaaga
     1081 ggttgtaggc cgccatgaca atcaagatgc cgtagtaagc tccgagtgcc atctgttcat
     1141 catgatcggc tgagtgaaaa gcctccggag accacacggc aagagggatc tgcatgctgt
     1201 cttcgttttg aacgcgtaaa tagtaagtcg tagaagccgg tttgattttg tagacgaacg
     1261 tgcgatgttc catgtcgcga gtggaaaacg gaaactgagt tccagtttgt gatacgagtc
     1321 tctcttcttc aaagagttct atgtgatcaa tcagagggaa agctacctgc agaaaccaca
     1381 gattttcatt ggaacgattt tcaaggcgcg cccgaatcca gtatgtggaa tgagtgaaac
     1441 caaagctcaa aacatcagag gaaccagggc gaaaacggag tcgcgccgat ccacttttta
     1501 catcgtggat agtcaactgt ccggacgcat cctcaagcac gtcgacctca cgacccagct
     1561 gacgatggcg aagatcgctt gtaagaatga tggggcgctc ctccgtagaa accggatcag
     1621 cttgcacccg gccagccaga gcaaagatgg tcagtgagat aaccagaacc ctgcgaacca
     1681 cactttgtgt ctagttctag aacatgaagg agcaaccaga atttcggttt catgaatttt
     1741 attttggttc gctggttgta aataattcga atcgaaaaga aaattcaagt aatgaaatag
     1801 gtgtccatcc gactaaagcc cgacggagtt ctgcttgata ctcgattgga accataacga
     1861 gcccggtgtc tgaaagatat ttgtatagaa atattactat ttcatcctga ggcagaacag
     1921 atctcgctca cagagccaga cacggtcgat ccagattcag cccctgaaac atcaggctcc
     1981 ccagagcatc ttcgcgccaa tggcaaccgc gaagacacca aaaatgcgtc gcagcatttc
     2041 agggttgatg gaggtcgcga acctcgcacc cacaaagccg cccacaatga atccgcctgc
     2101 gatccacatc gccgccggca cgtttacgaa ccctgctctg tagtatacat acgcggcaag
     2161 agctcctatg ggaggaacca tcgcggcaag agtcgttcct tccgcctgct gctggctgaa
     2221 ggcgaaaaag aaaaccagaa caggtacaag aatgatccct ccgccaattc caattagacc
     2281 actgatcgta cccgcaaaga atccaagagc caggaaaagc atattatcgt tcatgtaagc
     2341 tatacggctg ttgccatgcg aaagcgacag gattttaggg ttcacgcatc gacggccggt
     2401 tcg
//
LOCUS       DAOQJL010000586         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_32659_length_2399_cov_4.150171, whole genome shotgun sequence.
ACCESSION   DAOQJL010000586 DAOQJL010000000
VERSION     DAOQJL010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32659_length_2399_cov_4.150171"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLG78_18325"
     CDS             <1..428
                     /locus_tag="PLG78_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25137.1"
                     /translation="LAAKNFERAIQEYKNVLSVNAGNQEAMAGLDEAKLGISEQYRAR
                     GDAAVDPYDKARFYSSAVQYMPNNTRAAMALDGVRARQKQRLAEDLKLGIADYNAREF
                     DRAVKTFENILLIEPGNREAQEYLRIAVRKRDALRKLQE"
     gene            complement(438..>2399)
                     /locus_tag="PLG78_18330"
     CDS             complement(438..>2399)
                     /locus_tag="PLG78_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE25138.1"
                     /translation="LVGNNQRTLVYVDGILDNNLTENGVLGGTLRFPLYNVERIEVIG
                     GPASALYGANAFNGVINIITKDGTTPGGGVQALGGWSEDNFHNPGGGGSISARGTTGD
                     ADLRFSYSAAATYLKSAGPYFGDIQHLDKRNANANDAAYYTETQFCGGVCNPDAKSIG
                     YWWSKYYDSPEDTYNITARFSAGGFRFETINWQYLQGNGTFSNGTQQIDTDRIKGFRG
                     ASWDFRNNSASLGYLYAISSKVSLDSEITARHTDVLSSSHDEAPNSPGPYAYYRPYDT
                     TPSSNYERPDYAYVANERLLWEQTRGSNLTVGVESSFTVVPTAYGSARRFRYVNNAAY
                     LQQIWGPWEWLSLTAGYRYDYSTIYGAANTPRIGTVITPMKDLSIKLLASSGFRAPTA
                     WELFNATNQRKANPDLKPERLRSYEFGVGYRFFRRYYVSVQQYYNRINNLLLEVQTAE
                     PNPNQSGTNWNQNQNVGRATIYGTEVETDVQISNSLGLFLNYTYNHGKYEDLSYSLTS
                     SPSTRGRTGDDPLFDATAAAVKQTLGRSIVPESGSIPNIAPHHANAGLTWKILKNVTA
                     FFGINYVDIRRTVATNPEKTVPGYTMGTLNLRWEDAFLSGLDFSLLVRNAGNQQFFDP
                     GIRAATGSYYPTEHPLEKRNIWLTAGYRF"
BASE COUNT          543 a          553 c          663 g          640 t
ORIGIN      
        1 acctggccgc aaagaatttt gaaagagcga tccaggaata taaaaacgtt ctcagcgtga
       61 atgctggaaa tcaagaagcg atggccggcc tggacgaagc gaaactcgga atcagtgaac
      121 agtaccgcgc tcgcggagac gcggcagtcg atccgtatga caaagccagg ttttacagtt
      181 ctgcagtaca atacatgcca aacaacactc gtgctgccat ggcactggac ggagttcgcg
      241 ccaggcagaa gcagcgcctg gcagaggacc tcaaactggg gattgcggat tacaatgcgc
      301 gagagtttga ccgggcagtg aagacttttg agaatattct attgatcgaa ccagggaacc
      361 gagaagctca ggaatacctt cgcatagcag tgcgtaagcg agacgctctg agaaaacttc
      421 aagagtaaga gttctcttta gaatctgtat ccggcagtga gccagatgtt tcttttttct
      481 agcggatgct ctgtgggata gtaggagcca gtggctgccc ggattccagg atcaaagaac
      541 tgttgattgc ctgcgtttcg caccagaagg gagaaatcga ggccagaaag aaacgcatct
      601 tcccatctga ggttcaaggt tcccatagta tacccgggca cagtcttctc tggattggtt
      661 gccacagtgc gacgaatgtc cacatagttg atgccaaaga acgcggtgac gttcttcaag
      721 attttccagg taagtcctgc atttgcatgg tgaggcgcta tgtttggaat ggaaccggac
      781 tctggcacaa tggatctacc gagagtctgc ttcacggcgg cggctgtggc gtcgaagagc
      841 ggatcgtctc ctgttctacc tcttgtggag ggagaggaag tgagtgagta agacagatct
      901 tcgtatttgc cgtggttgta agtgtagttc aagaacagtc caagagagtt cgagatttgc
      961 acgtcggttt ctacttctgt tccgtaaatc gtggcgcgtc ctacattctg gttttgattc
     1021 cagtttgtgc cgctctggtt tggatttggc tctgccgttt gcacttcgag gagtagattg
     1081 ttgattctgt tgtagtattg ctgaacggat acgtagtaac gccgaaagaa acgatagccg
     1141 actccaaact catacgatcg cagcctttcc ggttttagat cggggtttgc ttttctttga
     1201 ttcgttgcat tgaatagttc ccaggctgtg ggcgcgcgaa atccgctgga agcaagcagt
     1261 ttgatggaca ggtccttcat tggagtgatt acagttccaa tgcgcggagt gtttgccgcg
     1321 ccgtagatgg tgctgtagtc atagcggtat cctgctgtca gagacagcca ttcccagggt
     1381 ccccagattt gctggagata ggcggcattg ttgacgtatc taaatcttcg cgcggaacca
     1441 tacgcagtgg gcacgactgt aaaagaagat tcaacgccta cagtgagatt ggatccgcga
     1501 gtttgttccc agagcagcct ttcgtttgca acgtatgcat agtccggtct ttcgtaattg
     1561 gaggaaggtg tagtatcgta cggtctatag taggcgtatg ggcccggact gttgggcgct
     1621 tcgtcgtgac tggagctaag cacgtctgta tgtctggctg tgatctcact gtcgagggat
     1681 actttagagc tgatcgcgta gaggtatccc aggcttgcgc tgttgtttcg aaaatcccag
     1741 gacgcgccgc gaaacccttt gattcgatcc gtgtctattt gttgcgttcc gttggaaaat
     1801 gtgccgtttc cctgcagata ttgccagttg attgtctcga atctgaagcc accggcagaa
     1861 aatcgcgccg tgatgttgta tgtgtcttca ggagaatcat agtatttaga ccaccagtat
     1921 ccaatcgatt tagcatccgg attacacact ccaccacaga attgcgtttc agtgtagtag
     1981 gctgcgtcat ttgcgttcgc atttcttttg tccaggtgct ggatgtctcc gaagtaaggg
     2041 cccgcagatt ttaaatacgt ggcagctgcg ctgtaactga atcgtaagtc cgcatctcct
     2101 gtggttccac gtgcgctgat ggatcctccg cctccagggt tgtgaaagtt gtcttcgctc
     2161 catcctccga gtgcctggac tcctccaccc ggcgtggttc cgtctttggt gatgatgttg
     2221 atgacaccgt tgaatgcgtt ggctccgtag agtgcggatg caggtccgcc gatgacttca
     2281 attctttcta cattgtagag agggaatcgc agagttccgc caagcacacc gttttccgtc
     2341 aggttgttgt cgagaattcc atccacatag accagagttc tctgattgtt tcccacaag
//
LOCUS       DAOQJL010000587         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_33081_length_2382_cov_4.045122, whole genome shotgun sequence.
ACCESSION   DAOQJL010000587 DAOQJL010000000
VERSION     DAOQJL010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33081_length_2382_cov_4.045122"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLG78_18335"
     CDS             complement(<1..902)
                     /locus_tag="PLG78_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNE25139.1"
                     /translation="MFQKALTLIFGSKHERDIRKLLPIVEKINSLEPSLKALSNEELG
                     AKTAEFKARLARGETLDDILPEAFACVREASVRTLGLRHFDVQLIGGMVLHQGKIAEM
                     KTGEGKTLTATSAIYLNSLSGKGVHIVTVNDYLARRDATWMKPIYDLLGVSVGVIQHN
                     LSTEERKQAYNSDITYGTNNEYGFDYLRDNMVSEPEYRVQRPHNFGIVDEVDSILIDE
                     ARTPLIISGPTDENTDKYASIDKAIIRLIQEEDKAPEPPPREVEPGKEEPHVIFGFYY
                     DVDEKSRNVTLTEAGVHKMEEILQV"
     gene            complement(946..1614)
                     /locus_tag="PLG78_18340"
     CDS             complement(946..1614)
                     /locus_tag="PLG78_18340"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNE25140.1"
                     /translation="MSYPRREIALGIFYQYSSYVIMQTFCLLAETTRGPTLPDRLHEW
                     IHADRTLAVYNSTIWLPGVLVFIAFLAIRSPMACVNYMRVGAVVTVFRGLFIFATSLG
                     PPLAVMSDAPPAMLSLTRESITLPLLIRQWIPLDVLYGGSGLSAAYLTQDLFFSGHTA
                     TTFLFCLVTRGWIRTVSVFFHIVTVALLILTHEHYTIDILGAYFVVYAVHSFFERRDL
                     LLSA"
     gene            complement(1611..>2382)
                     /locus_tag="PLG78_18345"
     CDS             complement(1611..>2382)
                     /locus_tag="PLG78_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25141.1"
                     /translation="GKALVTLADVTGNTADLRIEFQKGADVTRYALMEAPQRGNAAHA
                     SAPGGLIDVTTYGQNATLGLVAAPLPSGVLKEGLVQAGPSFYLSGRDLTMRDGLRLQY
                     HGQFANASLYVVHPVSRAVNLIGAGKAAGASTAFDFPMYRGEGWIVPLYDKIAPRVER
                     VMLYGGLTPDTKEDRDPETGGWFREYYISDAGSGVRPETLQVLLNGNSYPYTWKADRS
                     VVAVSVPRSFARDGALLSIRMEDVAGNRSDWFLDTIVP"
BASE COUNT          611 a          626 c          588 g          557 t
ORIGIN      
        1 acctggagaa tctcttccat tttgtgaaca ccggcttctg tgagagtcac gttgcgcgat
       61 ttttcgtcca catcgtagta gaaaccgaaa ataacgtgag gctcttcttt cccaggctct
      121 acttcgcggg gcggcggttc tggagcctta tcctcttcct gaatcaggcg aatgatggcc
      181 ttgtcgatgc tcgcgtattt gtccgtattt tcatccgtcg gacccgagat gatgaggggc
      241 gtgcgagctt catcgatcaa aatcgagtct acttcgtcca caataccaaa gttatgtggt
      301 ctctgaacgc gatactcagg ttcagaaacc atgttgtctc tcaagtagtc aaagccgtat
      361 tcgttgttcg ttccataagt aatgtcgctg ttataggcct gctttctttc ttccgtagaa
      421 aggttgtgct gaatgactcc cacgctcaca cctaacagat cgtagatggg cttcatccag
      481 gtggcgtcgc gtctggccag gtagtcattg actgttacaa tgtgaactcc cttacctgac
      541 aatgagttga gataaatagc gctggttgca gtgagagtct taccttctcc cgttttcatt
      601 tcagcgattt tgccctgatg aagaaccatg ccaccaatca actgcacatc aaagtgacgc
      661 aggcccagag ttcgaacgga tgcttcgcgc acacatgcga atgcttcagg gaggatgtcg
      721 tcgagtgttt cgcctctggc aagtcgtgct ttgaattcag ccgtctttgc accgagctct
      781 tcattgctca aagccttcag tgagggttca agtgaattga ttttttcaac gatgggcagg
      841 agtttgcgta tatcgcgctc atgctttgag ccgaagatga gcgtcagagc cttctgaaac
      901 ataatctgtc aaattccccg gcggacacaa ggaatccacc gtttttcagg cggataagag
      961 caagtctctt cgctcgaaga atgaatgaac agcatagact acaaagtaag cacccaagat
     1021 atcaatcgtg tagtgctcat gcgttaaaat gagtaaagca actgtgacta tatgaaagaa
     1081 cacagatacc gtcctgatcc acccccgggt tacgagacaa aaaaggaatg tggttgcggt
     1141 atgccctgag aagaaaagat cctgggtcaa ataggcggca gacaaaccac ttcctccgta
     1201 cagaacatcg agaggaatcc actgccgtat caggagggga agagtgatcg attcccgggt
     1261 caaagaaagc atggcgggtg gcgcatctga catcacagcg agaggtggac caagactggt
     1321 tgcgaaaatg aagagtccgc gaaatacagt gactactgcg cccacgcgca tatagttcac
     1381 gcaggccatg ggactgcgaa tcgccagaaa cgcaatgaag accagtactc caggtagcca
     1441 gatggttgaa ttgtatacag caagagtgcg atcggcgtgt atccactcgt gaagtcgatc
     1501 cggcaaagtg ggtccgcgcg tagtctccgc gagaagacaa aacgtctgca tgatcacgta
     1561 agaagaatac tgatagaaaa ttccgagtgc gatctctcgt cgtgggtaac tcatgggaca
     1621 atcgtgtcca aaaaccaatc ggaccgattg cctgcaacat cttccatgcg aatgctcaga
     1681 agcgcgccat cgcgcgcgaa ggacctggga acgctgaccg ccaccacact gcgatccgcc
     1741 ttccacgtat acgggtacga atttccgttc agcaagactt gcaaagtttc cggacgaact
     1801 ccgctgcctg cgtcagatat atagtattcg cgaaaccatc ctccggtttc tggatcacga
     1861 tcctcttttg tgtcaggcgt tagaccaccg taaagcatca cacgttccac tcgaggcgca
     1921 attttgtcat aaagcggaac aatccaacct tctccgcggt acatgggaaa atcaaatgca
     1981 gtggaagcac ccgcagcctt ccccgcgcca atcagattga ctgctctgga aacaggatgc
     2041 accacgtaga gagaagcatt cgcaaactgc ccgtgatact gcaacctgag gccatcgcgc
     2101 atggttagat ccctgccgct caaatagaaa gatgggccag cctgcacaag accctccttt
     2161 aagactccag acggcaatgg tgcggccacc agacccagcg tggcgttctg gccgtacgtc
     2221 gtcacatcga tcaaaccgcc aggtgccgat gcatgcgccg catttcctct ctgcggagct
     2281 tccatcaacg cataacgggt aacatctgcc cccttttgga attcaattcg aagatccgca
     2341 gtgtttcctg ttacatccgc cagagtcaca agtgcttttc cc
//
LOCUS       DAOQJL010000588         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_33087_length_2382_cov_3.340352, whole genome shotgun sequence.
ACCESSION   DAOQJL010000588 DAOQJL010000000
VERSION     DAOQJL010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33087_length_2382_cov_3.340352"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLG78_18350"
     CDS             complement(<1..321)
                     /locus_tag="PLG78_18350"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNE25142.1"
                     /translation="MMTARIAQWIPSFLKPRILDRYIFLEILAPFLVWLALLTTLFMS
                     IVLKDIAGELFGKGISPFKISVYIFYLIAEKITQTIPVACLFSGILAAGRLSGDSEIV
                     AMRSA"
     gene            305..1351
                     /gene="waaF"
                     /locus_tag="PLG78_18355"
     CDS             305..1351
                     /gene="waaF"
                     /locus_tag="PLG78_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNE25143.1"
                     /translation="MRAVIIQTAFLGDAILTTPMFAAFKRFFPDSYLAAVVKPDAAQV
                     LKGLPYLDEIISFDKKKSHRGISGLRKLAADLQSRDFDILLSPHSSYRTAFLSRLSKI
                     PERYGYLDASFSRIAYNHRLPRHSSQPEILRLLSFLSDSVCPDASKASTDLVLAEDQA
                     CVLEAESVLRKHEASRPILLAPSSVWATKRWTPAGFAELAGKLVRKHKCKILLVGSPS
                     DKAICEQVMAFIRDLQPLFVQDRVTVAAGETSLRGFYSLVKRSRLLVSNDSAPVHYAC
                     AAKTPVVALFGPTVPALGYAPITKNSRIAEINLACRPCGTHGGKVCPLSHFRCMKELT
                     ADMVMAKVSELLPE"
     gene            complement(1644..>2382)
                     /locus_tag="PLG78_18360"
     CDS             complement(1644..>2382)
                     /locus_tag="PLG78_18360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE25144.1"
                     /translation="KGLLISIVIVLCILVGWNLFGSESGEGGGIDSIAEPGRRALLLN
                     VKENISLVRRLEQNQREIHQILKDEPELFGKFKSDSEPPYDWSRPSAMSMLRANADYA
                     GIYSGFFREIPHRWPLRLDFLKLNSGYGSRGAVFGFLGQSEFHPGFDLNAVAGTPVLA
                     AADGVVKLALSSSYGYGNHVVILHSSGYETLYGHLRSISVKKGQEVRASQEIGKSGSS
                     GASTGPHLHYEIRFAGKTVHPGDFLVF"
BASE COUNT          607 a          634 c          557 g          584 t
ORIGIN      
        1 agccgatcgc atggctacga tttcactgtc tccggaaagc ctgcctgcgg cgaggattcc
       61 tgaaaacaga caggcaactg gaatggtctg ggtgattttc tcagcgatca gatagaagat
      121 atagactgag attttgaaag gggaaatccc tttgccaaac agttcccccg caatgtcttt
      181 gaggacaatg gacataaaaa gcgtggtcag gagggccaac cagacgagga atggtgcgag
      241 gatttccaga aaaatgtagc ggtccagaat tctgggcttc aaaaacgacg gaatccattg
      301 cgcaatgcga gcagtcatca ttcagaccgc ctttctcggc gatgcaatac ttaccacgcc
      361 tatgtttgcg gccttcaagc gtttttttcc cgattcgtat ctggccgctg tagtaaaacc
      421 agatgcagcc caggtcctca aaggccttcc ctatctagac gaaatcatat cctttgataa
      481 gaagaaatca caccgcggga tttctggcct tcgaaaactc gccgcagact tgcaatcgcg
      541 tgattttgat attctacttt ctcctcattc atcttatcgc actgcatttc tttcgcgtct
      601 ttctaaaatt ccagagagat acgggtatct ggacgcgagc ttttcgcgca tcgcctacaa
      661 ccatcgcctg ccaaggcatt cttctcagcc agaaattcta cgattgcttt cctttctttc
      721 ggattcagtc tgtccggacg cgtctaaagc atctacggac ctggtgcttg cagaagatca
      781 agcatgcgtg ctggaagcgg agtctgtgct gcgaaagcac gaagcatcca gacccatttt
      841 gctcgcacct tcttctgtct gggcgaccaa gcgctggact ccggccggat tcgcagaact
      901 ggcagggaag ctggttcgaa aacacaaatg caagattcta ctcgttggat ctccttcaga
      961 caaagcgatc tgtgaacagg tgatggcatt cattcgagat cttcagccac tctttgttca
     1021 agatagagtc actgtggcgg ccggtgaaac atcgctgcgc ggcttttatt cacttgtaaa
     1081 aagatcgcgc ctccttgtat caaacgattc tgcgccagtt cactatgcct gtgccgcgaa
     1141 gactcctgtg gtcgccttgt tcggaccaac ggtgccggcc ctgggctacg caccgattac
     1201 aaagaattcg cgaatcgcag aaatcaatct ggcatgcaga ccatgcggca cgcatggagg
     1261 aaaagtctgt cccctttccc attttcgctg catgaaagaa ctcacggcgg acatggtgat
     1321 ggcaaaagtt tcagagcttc taccggaata agatccgcag cggacacctg cgcgccagga
     1381 atcctgcacg tggtcaatac gcggcgcacc cattcgcaat accagagaat gatacgtatt
     1441 ccatacgatc tggttttccg ctcgttcccg gcgctttttt cacgacccga cgcattcgcc
     1501 gcgttttata cgcactcgat gtaatacgaa caaagaatgc gcgatattgc gcacgcccgt
     1561 atgtaatacg tcattttatt gcgtgataaa accgcgggat ggcgtgcggg aacgacaagg
     1621 cgctgcaggc ccccggagcg attttaaaag accagaaagt ctcctggatg aactgtcttt
     1681 cctgcgaatc gaatctcata gtgaagatgc ggacctgtgg acgctcctga ggatccggat
     1741 ttaccaatct cctgactggc acgcacttcc tgccctttct tcacagaaat ggatctcaga
     1801 tgaccgtata acgtttcgta accggaagaa tgcaaaatga ctacatggtt gccataacca
     1861 tagctcgatg aaagagcaag cttcactaca ccatccgctg ccgcaagcac cggggttccc
     1921 gccacagcat tcaaatcgaa tccgggatga aactcactct ggcccaggaa accaaagaca
     1981 gctcctctgc ttccatatcc tgaattcagt tttaaaaaat ccaggcgaag aggccagcgg
     2041 tgaggaatct ctctaaagaa tccggaatag atccctgcat agtctgcgtt tgcacgaagc
     2101 atgctcatcg cactgggtct gctccaatcg taaggaggtt cagaatcgct tttgaatttt
     2161 ccaaatagtt caggctcgtc tttgagaatc tgatgaatct ctctttgatt ttgctcgagc
     2221 cttcgcacga gtgaaatgtt ctcttttaca tttaaaagaa gagcacgcct gcctggctcg
     2281 gcaatcgaat caatgccgcc gccttctccg ctttcagaac caaagagatt ccagcccacg
     2341 agaatacaca atactatgac gatggaaatc aatagccctt tc
//
LOCUS       DAOQJL010000589         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_33984_length_2349_cov_5.124237, whole genome shotgun sequence.
ACCESSION   DAOQJL010000589 DAOQJL010000000
VERSION     DAOQJL010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33984_length_2349_cov_5.124237"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLG78_18365"
     CDS             complement(<1..349)
                     /locus_tag="PLG78_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25145.1"
                     /translation="MKAPVLPILLLLVGTACTPPFSVNPSPPQNLIALGILLGAPTYL
                     TPADISNTRMWYRTETLESTYTNGSPITNWTNSISAGTYLIANVGAARPTMDTSVFGG
                     HASALFSAASSQAL"
     gene            455..1495
                     /locus_tag="PLG78_18370"
     CDS             455..1495
                     /locus_tag="PLG78_18370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE25146.1"
                     /translation="MKRFLHRLRTLAWISLFVGSVSSFFALFQEGSFSPTYLIYGLLD
                     GVIIGALIAFYVLFIVRGTFSDLARRTPFVLLLTINTAAYVLVFVLARAWAIWITGRG
                     PFDILPYDRGHFQSAAIFALIISLTINFFMQISQLLGQNTLWNFLLGKYHRPASEFRF
                     VMYLDLAGSTSIAEKLGDKQFLALLNDFFYDMTDAVLETGADIYKYVGDEAILLWTRK
                     RGVKRNDALRFIAAFARTLRDAEQKYALRYGLVPEFRAGLHYGQIVAGEMGNLRQEVA
                     YMGDAMNTGARVLDVAKKLKKTFIVSEDALSQFNVPGLEDLGEHELRGKEKRIRLYGA
                     PDSLFEEFRRLE"
     gene            complement(1498..>2349)
                     /locus_tag="PLG78_18375"
     CDS             complement(1498..>2349)
                     /locus_tag="PLG78_18375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNE25147.1"
                     /translation="NFKNIVDTCRVKVPFGVAQIGKSFRNEIVARQFVFRTREFEQME
                     MEYFCAPGTQKEWFEHWVEYCMNFLLRIGIKKENLRIRRHEAEELAFYSEGTVDIEYN
                     FPFGWGEIWGIASRTDYDLKKHAEFSGKPLEYHDSEKNEKYIPYVVEPALGLNRLMLA
                     VLCDAYYVEKEGTPEARTVLGLAPTIAPVKVGVFPLMKKDGLPEIAREIYKTLSGHFQ
                     VEYDVSGNIGKRYYRQDELGTPFCITVDYDTKEDQTVTLRERDSTKQERVKISDLLSI
                     LRERMAI"
BASE COUNT          584 a          583 c          563 g          619 t
ORIGIN      
        1 taagggcttg cgagcttgct gcactgaaca aggcagaagc gtgtccaccg aaaacggatg
       61 tgtccatcgt tgggcgtgcc gctcccacat ttgcaatgag gtacgtgccg gcagagatag
      121 aattggtcca gttggtgata ggagagccgt tggtgtacgt ggattcaagc gtttccgtgc
      181 gataccacat acgagtattg gaaatatctg ccggagtcag atacgtaggc gcacccagca
      241 gaatcccaag ggcaatcaag ttctgcggag gactgggatt gactgaaaac ggtggagtgc
      301 atgcagtccc gactagcaaa agcaagatgg gcaggaccgg tgccttcatg atacgcaatt
      361 gaatcattcg gaagaaattg gagcaatgac aattcctgtc gcttagcctg gtttttttct
      421 gttcccggcc atccctcccg gttcagaaag tagcgtgaaa cgattcctgc acagactccg
      481 cacactcgcc tggatctccc tgttcgtggg aagcgtgagc tccttcttcg ctctttttca
      541 agaaggcagc ttctctccta cgtatctgat ttacggactg ctggatggcg tgatcattgg
      601 ggcgctcatt gctttctacg ttctgttcat cgttcgcgga acgttctctg atctggcacg
      661 gcgaacaccc ttcgtactgt tacttacaat caatacagca gcgtacgttc tggtttttgt
      721 tcttgcgcga gcctgggcta tctggatcac aggtcgaggg ccatttgata ttctcccgta
      781 cgaccgtggg cactttcaaa gtgctgcgat ctttgcattg ataatttctc ttacgatcaa
      841 tttcttcatg cagatcagtc agcttctagg acaaaacact ctgtggaatt ttcttctggg
      901 taagtatcat agacctgcat ctgagtttcg ctttgtcatg tatctggatc ttgccggttc
      961 cacgagcatt gcggaaaaac tcggagacaa acaattcctg gcgcttctaa acgatttctt
     1021 ctatgatatg acagacgcag ttctggaaac cggagcggat atctataagt acgttggaga
     1081 cgaagcgatt ctactctgga ccagaaagcg cggcgtcaaa aggaatgacg cactgcgatt
     1141 cattgccgca ttcgcgcgaa ccctgcgcga cgcagaacaa aagtacgcac ttcgatatgg
     1201 actggttccc gagtttcgcg ccggcttaca ttacggccaa atcgtggccg gagaaatggg
     1261 aaatctcaga caagaagtag catacatggg agacgcgatg aacacaggcg cgcgcgttct
     1321 tgacgtcgca aagaaactca aaaagacgtt catcgtatct gaggatgctc tttcgcaatt
     1381 caatgtcccc ggactcgaag acctgggcga acatgagctg cgaggaaaag aaaagcgcat
     1441 ccgcctgtac ggtgcgcctg attctttatt tgaggaattt cgcaggctgg agtgaactca
     1501 gattgccatc cgctcgcgca gaatagaaag caagtctgaa atcttcaccc gttcctgttt
     1561 tgtagaatct cgctcgcgca gagtaaccgt ctgatcttct tttgtgtcgt agtctacagt
     1621 gatgcagaat ggtgtgccca attcatcctg acgatagtat ctcttgccga tatttcctga
     1681 cacgtcgtat tccacctgaa agtgacctga aagcgtttta tagatttcac gagcaatctc
     1741 tggcagaccg tctttcttca tgagtggaaa cacacctact tttacaggag caatggtagg
     1801 agcaaggccc agcacggtac gtgcttctgg cgtaccttct ttttcaacgt agtatgcatc
     1861 acaaagaact gcaagcatca gacgattgag tccgagcgcc ggctccacaa cgtatggaat
     1921 gtatttttcg tttttctctg aatcatggta ctcgagaggc ttgccggaaa attctgcatg
     1981 cttttttaga tcgtaatccg tacgagatgc gatcccccag atctctcccc aaccaaaagg
     2041 aaaattatat tcgatgtcca cagtaccttc tgagtagaag gcaagttcct ctgcttcatg
     2101 cctgcgaatg cgaagattct ccttcttaat gccaattctc aagaggaaat tcatgcaata
     2161 ctcgacccag tgttcaaacc attctttctg agttcctgga gcgcagaaat attccatttc
     2221 catctgttca aactcgcgcg tgcgaaacac aaactgcctg gccacaattt cattgcgaaa
     2281 gctcttccca atttgagcca caccgaacgg cacttttacg cgacaggtat ccacgatatt
     2341 cttaaaatt
//
LOCUS       DAOQJL010000590         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34093_length_2345_cov_4.956769, whole genome shotgun sequence.
ACCESSION   DAOQJL010000590 DAOQJL010000000
VERSION     DAOQJL010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34093_length_2345_cov_4.956769"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLG78_18380"
     CDS             <1..354
                     /locus_tag="PLG78_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25148.1"
                     /translation="ADATHIYYFGHAMMVDGSPAIPARDAWIPLLSRSMGDVSRKTVF
                     LGACLRQAGMTLRIPGGRFIYPITRIADRPNSFLERIAGSSHLLQGFHSACIRDAEEG
                     DIRRFVYRVKGSIWL"
     gene            399..758
                     /locus_tag="PLG78_18385"
     CDS             399..758
                     /locus_tag="PLG78_18385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE25149.1"
                     /translation="MDAAVRLRAPNQQKRKKARAQVNLPADFQILGHHEFLPCSLADI
                     GSGGLALITRSSLYIGDKLKVRFKIAESPFEGQCVVVRVVGKAVGVQFENLSPADEDR
                     LQDFIHKVFLERERKKP"
     gene            807..1091
                     /locus_tag="PLG78_18390"
     CDS             807..1091
                     /locus_tag="PLG78_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25150.1"
                     /translation="MEKVYCANCAHCIVVRQYEAEQDKYILRVRCDKKKWSKRSGEEK
                     LYKYFTVARRMQPNCEYYRDMGDLLPYIKNLKKELPIKDEIYSVKKSARP"
     gene            1113..1580
                     /locus_tag="PLG78_18395"
     CDS             1113..1580
                     /locus_tag="PLG78_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNE25151.1"
                     /translation="MELKDIIPPDRIFYIERSFKSKEELLSHLLEQLLKKDGLEAHKD
                     QVLKVLLERESSMSTGIGQGIAIPHCSTDFEKDLSAVLAVLKKPIEFQAVDEQPVQIV
                     ILLVLPKDRFEKHIKTLAAIARQFNDSVFRKKVAGAASVDEVQAAVAAATASA"
     gene            complement(1584..2159)
                     /locus_tag="PLG78_18400"
     CDS             complement(1584..2159)
                     /locus_tag="PLG78_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE25152.1"
                     /translation="MNKNPMEEFAGLQNELDKRFLDQRKIFLWGAVEDESARHIVERL
                     LYLESQAPGKDIQFYINSPGGVITSGMAILDTMNMITSDVSTICMGLAASMGALLLCA
                     GKKGKRFGFPHARVMIHQPLISGQIVAPAIDIKIHAEEIKKTRDEINRIIANATGKAL
                     EQVEEDTDRDYYLTAKEATEYGMIDGVIDRI"
BASE COUNT          562 a          567 c          615 g          601 t
ORIGIN      
        1 gcagatgcta cgcatattta ctactttgga catgccatga tggtggatgg aagtcccgcg
       61 atacccgccc gggatgcctg gattcctttg ttgagccgga gcatgggaga cgtatctcgc
      121 aagaccgtgt ttttgggtgc ctgtctccgc caggctggca tgactcttcg cattcctggg
      181 ggtagattca tctatccaat cacaaggatt gcagatcgcc ctaactcctt cctcgagcga
      241 attgccgggt ccagtcatct tttgcagggc ttccattctg cctgcattcg cgacgcagag
      301 gaaggggaca ttcgtcgttt tgtttaccgc gtgaaaggct ccatttggct ttgacaaatc
      361 aggcgcatgt cgcaggtttc ggtggccggg gggaatttat ggatgcagcc gtgcgcctca
      421 gagcgcccaa ccagcagaag agaaagaagg ccagagcgca ggtcaacctg cctgcggact
      481 ttcaaatcct gggacatcac gaattcctgc catgttccct tgcggatatt ggatctggcg
      541 gcctggctct gatcactcgt tcttctcttt acattggaga taagttgaag gtgcgattca
      601 agatcgccga gtctccgttt gaagggcagt gtgtggtcgt gcgagtcgta ggaaaggcag
      661 tgggagtgca gttcgagaat ctcagcccgg cagacgaaga caggctgcag gactttatcc
      721 acaaggtctt cctggagaga gaacgcaaaa agccttgacc ctgaggcttc ctcgcgaaag
      781 ctggcctttg aagaaggcag gcatccgtgg aaaaagtcta ctgcgccaat tgcgcacatt
      841 gcatcgttgt aagacagtat gaagccgagc aggataagta catcctccgt gttcgttgcg
      901 acaagaagaa gtggtccaag cgttccggcg aagaaaagct ctacaagtat ttcacagttg
      961 cccgccgtat gcagccaaac tgcgagtatt acagagacat gggagacctg cttccataca
     1021 tcaagaacct caaaaaggaa ctcccgatca aagacgagat ctactctgtc aaaaagtccg
     1081 ctcgtccttg agttcgcacc tatggtgtga ccatggaact gaaagacatc ataccgccag
     1141 accgcatctt ttacatagag cggtctttta aatcaaaaga agaactactc tcgcaccttc
     1201 ttgagcagct gctcaagaag gatggactcg aagcgcacaa agaccaggta ttgaaagtac
     1261 tcctggaaag agagtcttct atgtccacgg gcataggcca gggcatcgcc attcctcatt
     1321 gttccactga cttcgaaaaa gatctgtctg cagttctagc agttctcaag aagccaatcg
     1381 agtttcaagc agtggatgag cagcctgttc aaatcgtaat tcttctggtt ctgcctaaag
     1441 atagattcga gaagcacatc aagacgcttg cggcgattgc ccgtcagttc aatgattctg
     1501 tgtttcgtaa gaaagtggcg ggtgcggctt ctgtagacga agtgcaggct gccgttgcgg
     1561 ctgcaacggc atcagcttaa aagttagatc ctgtcaatca ctccatcgat cataccgtac
     1621 tcagttgctt ccttcgcagt gaggtagtag tcgcggtctg tatcttcttc tacttgctca
     1681 agagctttgc ccgttgcgtt tgcaatgatg cggttgattt catcgcgagt cttcttgatt
     1741 tcttctgcgt gaatcttgat gtcgatggcc ggcgctacaa tttggcctga aatgagaggc
     1801 tggtgaatca tcactctggc gtgtggaaaa ccaaaccgtt tgcctttctt tcctgcgcag
     1861 aggaggagtg cgcccatact tgctgccaga cccatacaga tcgtggaaac atcggaagtg
     1921 atcatattca ttgtatctag aattgccatt ccagaggtga tcactccccc cgggctattg
     1981 atgtagaatt gaatgtcttt gcctggtgcc tgggattcaa gatagagcag gcgctctaca
     2041 atgtggcgcg ctgattcatc ttctactgcg ccccagagga agattttgcg ctgatcgaga
     2101 aatctcttgt ccagttcgtt ttgaaggcct gcaaattctt ccataggatt tttgttcata
     2161 agttcagagg tggttcctgg tgtgttgtgt caaaagcttt ttcgtccaac aggcagcatg
     2221 cgaaggagca ccatgccaga ggcactgagc agtgacatca caagaaagaa aacggtttca
     2281 ggatcggcaa gtgttcgttc catcttcgcg gaaaacattg cgggatctga ctgagtgttt
     2341 gtttc
//
LOCUS       DAOQJL010000591         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34188_length_2342_cov_3.178400, whole genome shotgun sequence.
ACCESSION   DAOQJL010000591 DAOQJL010000000
VERSION     DAOQJL010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34188_length_2342_cov_3.178400"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1737
                     /gene="flgE"
                     /locus_tag="PLG78_18405"
     CDS             346..1737
                     /gene="flgE"
                     /locus_tag="PLG78_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNE25153.1"
                     /translation="MMRSLYSGVAGMKNHQTRMDVIGNNVSNVNTHGFKTERVTFMDM
                     ISQEISGAAEPRENVGGINPQQVGLGMMIASIDKIMTQGSLQTTGKNTDVAISGDGFF
                     VVRDGGKQYYTRAGIFSMDRDGFYVHPGTGLRVQGWNARQDRDGNSYINSAAGVEDIR
                     IPLYQKKPAKATSEVVFESNLNQAVQSVPADATEEQIQQFLSGPQETRRGHVATANVY
                     DEEGNKRELRLEFYKTGDNRWQVRANMENSSNLSVNVVGPGGQDTAVGGTNAFELGFA
                     SDGRVQSVSDGVDTQNSGKLTVNVSFRIPGNPAVQTIKLNMGEAGAVKGVTQFASSFT
                     THAKEQDGYPMGYLESFSIDNTGSIVGTYTNGVKEPLAKVALATFTNPAGLTKEGETK
                     FSESMNSGNANVGEAGIGGRGKMFAGVLEMSNVDLSDQFTDMIVTQRGFQANSRTITT
                     SDQMIQEILGLKR"
     gene            1914..>2342
                     /locus_tag="PLG78_18410"
     CDS             1914..>2342
                     /locus_tag="PLG78_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE25154.1"
                     /translation="MNPDLKIRFLRAMLHARISDEAEQALKRKGLVPFELSSEGHEAL
                     AAIAFAMGPDDWLHPHYRDRAIVLARGVSDREIFLDFFSRSGAPSAGRQMPVHYNSRA
                     LRIVTLSTPVATNALQAAGMALSLKERGIPEVVIASIGDAS"
BASE COUNT          624 a          594 c          628 g          496 t
ORIGIN      
        1 gcggatccgc agcaaatcct ccgcctgttc caccaccggc agggcagagc ccggtgcagc
       61 ccgcacattc gcagtctcaa acatcatcac ctgcgccagc actgcaaaaa acggacgagc
      121 acgcagctcc tacaaaatcg gaagttccat tgaaatcgga agggccggcg aatggggcgc
      181 ctccagagaa aaatcctgag aaaaaaggac ctgtctctct gcgcggatac ggtgattttt
      241 ctttcgcacc aaattttgaa tttgtcagct gaattgatta aactatttcc ctggaacgtc
      301 gagcagaaga aagcaactag aaacggataa ggaaaaacaa agactatgat gcgatccctt
      361 tactccggtg tagccggaat gaaaaaccac cagacacgaa tggacgtaat cggtaacaac
      421 gtttcgaacg tgaacacgca tggtttcaag acagagcgcg taacgttcat ggacatgatc
      481 tctcaggaga tctcgggtgc cgcagagccg cgcgaaaacg tgggcggtat caacccgcag
      541 caagttggtc ttggaatgat gatcgcatct attgacaaga tcatgaccca gggttctctt
      601 cagacaacag gaaagaacac tgacgtggcg atttccgggg acgggttctt cgtagttcgc
      661 gacggcggaa agcagtacta tactcgcgca ggtatattca gcatggatcg cgacggcttc
      721 tatgttcatc ctggaactgg acttcgtgtt cagggctgga acgcgcgtca ggatcgcgat
      781 ggaaacagct atatcaattc cgcagctggc gtagaggaca ttcgcattcc gctctatcag
      841 aagaagccag ccaaagcaac cagtgaagta gttttcgaat ctaacctcaa tcaggcagta
      901 cagtcagtgc ctgcggacgc aacagaagaa cagatccagc aattcctgag tggacctcaa
      961 gagactcgtc gtggacacgt ggccactgca aacgtatatg acgaagaagg aaacaagcgc
     1021 gaacttcgtc ttgaattcta caagacaggc gacaaccgct ggcaagtgcg tgcgaacatg
     1081 gaaaactctt ccaacctcag tgtaaacgta gttggtccgg gtggccagga cactgcagtt
     1141 ggtggaacga atgcatttga actcggcttt gcctccgatg ggcgcgtgca gtccgtctca
     1201 gacggagtgg acacacagaa ttcaggcaag ctcacagtga acgtaagctt tcgcattcca
     1261 ggaaacccgg ctgttcagac catcaaactg aatatgggcg aagcgggcgc ggtgaaaggc
     1321 gtcactcaat ttgcgagttc ttttacaacg catgcaaaag agcaggatgg gtatccaatg
     1381 ggatatcttg aatccttcag catcgacaac accggaagca ttgttggaac atacacaaac
     1441 ggcgtaaaag agcctctcgc caaagtagct ctcgcaacat ttactaaccc ggccggtctc
     1501 acgaaagaag gcgaaaccaa gttcagcgaa tcgatgaaca gtggtaatgc gaacgtgggt
     1561 gaagcaggaa tcggtggacg cggcaagatg tttgcgggtg ttcttgaaat gtcaaacgtg
     1621 gacctgtctg atcaattcac agacatgatt gtcacgcaga gaggtttcca ggccaactct
     1681 cgaaccatca caacgagtga tcagatgatc caggaaatcc tgggactcaa gcgctaggcc
     1741 tgagtgacta aaaacccgcc attcaggcgg gttttttatg cgatcatggc cggattatgt
     1801 cacatggccg ggcggccgga ttatgtcaca taatctccag gcgcccagac aaaagatctt
     1861 gcctgctcca ggaaatcatg cgaacctttg catgtaatct ctggaggcgg cgtatgaacc
     1921 ccgacctgaa gattcggttt ctgcgtgcaa tgctccacgc tcgcatcagc gatgaggcag
     1981 agcaggcgtt gaagagaaaa ggcctggttc cttttgagct atcaagcgaa ggccacgagg
     2041 ctctagcggc cattgctttt gccatgggac ccgacgactg gcttcatcct cattacaggg
     2101 atcgcgcgat tgtgctggct cgaggtgtgt ctgatcgcga gatctttctg gatttttttt
     2161 ctagatcggg cgctccgagt gccggccgcc agatgcctgt gcattacaat tctcgcgctc
     2221 ttcgcatcgt cacattgtcc acgccggtgg cgaccaatgc actgcaggct gctggcatgg
     2281 ctctgtctct caaagaacga ggaataccgg aagtggtgat cgcatccatt ggagatgcgt
     2341 cc
//
LOCUS       DAOQJL010000592         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34527_length_2329_cov_4.727353, whole genome shotgun sequence.
ACCESSION   DAOQJL010000592 DAOQJL010000000
VERSION     DAOQJL010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34527_length_2329_cov_4.727353"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLG78_18415"
     CDS             complement(<1..286)
                     /locus_tag="PLG78_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNE25155.1"
                     /translation="MKSNEIDRLREKIDQLDRSIVDLISQRTAVAEEIGQIKKRQDLP
                     IFRPDRHGAVYDRVADSVPEAMASGIRYIYREIMSLGMAVEGDLVFLCPDA"
     gene            complement(273..806)
                     /gene="scpB"
                     /locus_tag="PLG78_18420"
     CDS             complement(273..806)
                     /gene="scpB"
                     /locus_tag="PLG78_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNE25156.1"
                     /translation="MARYRGLIEAVLFVATEPQTVAMIARTINLDRVNTRILADSLVD
                     DFAERDGGISLREIAGGYQFVTSDRYSEEMREILQTSKREKLSRSVMETLAIISYRQP
                     ITLPEIEDVRGVASRPHVVTLLAKRLIKPQGTRPVPGRPTLYVTTKTFLQHFALNSLA
                     ELPPLEELKELKFDEIE"
     gene            complement(911..1678)
                     /locus_tag="PLG78_18425"
     CDS             complement(911..1678)
                     /locus_tag="PLG78_18425"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNE25157.1"
                     /translation="MVSVASPSEEVAGFLVSWVGPDGEEKGPLTLLWDLIESYRIDIF
                     DVSLFKITEDFMSYMKSAGLTLEQASSFTVMAARLLYYKSRALLPDPGFEEETEDRLP
                     PELIQQLLEYRKFQFASDKLRDLEEITSGMMRRRVTDEVTGEEYFEASLSDLVHAYTM
                     VLARIKQEHEEPALYHIRITEVSVEEKVAHIRRIFENAVSCLFTDLLVDLEQRGSGEV
                     IATFLGILELTKLGEIVLRQKVNFGQIEVHKKSYVLV"
     gene            complement(1682..2041)
                     /locus_tag="PLG78_18430"
     CDS             complement(1682..2041)
                     /locus_tag="PLG78_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE25158.1"
                     /translation="MARVLIVDDAKFMRTLVRDALTKAGHEIVGEAENGNQAVDLYKN
                     LKPDLVTMDITMREKDGLDAASEILKSDGRARIIMVTALGQEDLLARAIKMGVKDFVV
                     KPFPPERLQQAAAKALS"
     gene            complement(2093..>2329)
                     /locus_tag="PLG78_18435"
     CDS             complement(2093..>2329)
                     /locus_tag="PLG78_18435"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNE25159.1"
                     /translation="LNRVVDEYGMRAAALIMTGMGQDGLEAVTRLYQAGGLTLAQDEA
                     SSVVFGMNRRAIEAGVIDRVLTLEESAAVLRSFF"
BASE COUNT          557 a          612 c          548 g          612 t
ORIGIN      
        1 gagcatccgg acacaagaaa accagatccc cttccactgc cataccaaga gacatgattt
       61 cgcggtaaat ataacgaatg ccagaagcca ttgcctctgg aactgaatca gccacgcgat
      121 catacacggc gccatgcctg tcggggcgaa agatgggaag gtcctgacgc ttcttgattt
      181 gtccgatctc ttctgcaaca gcagtacgct gcgaaatcaa atcgactatg gagcggtcga
      241 gttgatcaat cttctctctg agcctgtcga tttcattcga tttcatcgaa cttcaattcc
      301 tttagttctt ccaggggtgg caattctgcc agagagttca acgcgaaatg ttgcaggaaa
      361 gtcttcgtgg ttacgtaaag agtaggtctt ccggggacag gtcttgttcc ctgtggcttg
      421 atcagtctct ttgcaagcag agtcaccaca tggggacgcg aagcgacgcc gcgcacatct
      481 tcgatttctg gaagagtgat aggctggcga tatgaaataa ttgcaagtgt ttccatcaca
      541 gaccgactca gcttttctct cttgctggtc tgaagaatct ctctcatctc ttcgctgtag
      601 cgatctgatg ttacaaactg ataaccgcca gcaatctcgc gaagactgat gccgccgtct
      661 ctttctgcaa agtcatccac aagactgtcc gcaagaatcc ttgtattgac tcgatccaga
      721 ttgattgtgc gagcaatcat ggctacagtc tggggctcag ttgccacaaa caaaaccgct
      781 tcgatgagac cgcgataacg tgccatctcc tccgcaccca ggtccctgaa tgccggaagc
      841 cctgcgtcat cgcctgaaat gtgaagttct actttctctt cgccagtctg ttctttgtct
      901 tcagccatga tcaaacaagt acatatgatt ttttgtgaac ttctatctgc ccgaaattca
      961 ctttttgccg cagcacaatt tcgcccagct ttgtaagttc aagaattcca agaaacgtgg
     1021 cgatgacttc acctgaaccg cgctgctcaa gatctacaag caagtccgtg aacaaacagg
     1081 aaaccgcgtt ctcgaaaatc cgtctgatat gtgctacttt ttcttccacg cttacttcag
     1141 tgattcggat atgatagagt gcgggctctt catgctcctg cttgattcgc gcaaggacca
     1201 tcgtgtatgc gtgaacgaga tccgaaaggc tggcctcaaa gtattcttcg cctgtaactt
     1261 catccgttac gcggcgacgc atcatcccgg atgtgatttc ttctaaatcg cgaagcttgt
     1321 cagatgcaaa ttgaaacttt ctgtattcaa ggagctgctg gatgagttca ggaggaagtc
     1381 tgtcttctgt ttcttcttcg aatccaggat ccggcaacag tgcgcgactt ttgtaataga
     1441 gaaggcgcgc ggccatcact gtgaatgaac tcgcctgttc gagagtgagc cctgcggatt
     1501 tcatatagga catgaagtct tcggtgattt tgaaaagaga tacatcgaag atgtcgatgc
     1561 ggtaagattc aattagatcc cagagaagag tgagagggcc cttttcctct ccgtctggtc
     1621 cgacccagga aactagaaag ccagcgactt cctcggatgg gctggcaacg gaaaccatga
     1681 ctcaggaaag tgctttcgcg gcggcttgct gaaggcgctc aggcggaaat ggttttacta
     1741 caaaatcttt cacgcccatc ttgatcgcgc gggcgagcag gtcttcctgg cccagtgcag
     1801 ttaccataat gattcgagcg cggccatctg acttgagaat ctctgatgca gcgtccagac
     1861 catctttttc acgcatggtg atgtccattg tgaccaggtc cggtttcaga tttttgtaaa
     1921 ggtctactgc ctggttgccg ttttcagctt cgcccacaat ttcgtggcct gccttggtga
     1981 gagcgtcgcg tacgagcgtc ctcataaact ttgcgtcgtc tacaatcagt actctagcca
     2041 tcttttatcg aaacccacag gaatgatttt tgagatcatg catcagtgca atctaaaaaa
     2101 aactgcgcag cacggcggct gattcttcca gggtaagcac ccggtcaatg actccagctt
     2161 cgatcgcccg gcgattcatg ccaaaaacaa cgctagaagc ctcatcctga gcaagtgtaa
     2221 gcccgcctgc ctggtagagc cgtgtcactg cttccagtcc atcctgcccc attccagtca
     2281 taatcagggc tgccgctcgc attccgtatt cgtccaccac tcgatttag
//
LOCUS       DAOQJL010000593         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34747_length_2321_cov_4.463813, whole genome shotgun sequence.
ACCESSION   DAOQJL010000593 DAOQJL010000000
VERSION     DAOQJL010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34747_length_2321_cov_4.463813"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLG78_18440"
     CDS             <1..902
                     /locus_tag="PLG78_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25160.1"
                     /translation="KLRVRVLLLPEGMDAFDLSVKTDAATILSLLALSFPGERYLIVE
                     TMFPGTAVIPAAEPGSEGLLRFGEHVRREYERKRMELDASSKRDGLARLDELVKTMPP
                     ELGQILKQEGSAIIGVSPDRARSPMQKRPAQGRTVMAPPLSNIAKCEREIVAIFMFHP
                     TALVDLREEMDSFSFQDEPSEIMVRYLDTRAISGNLWTPETVFSDQLPDSIVSIFHGL
                     LLSRAPSEDPVGVVRDLLVEHRIRSLEKEFEDLDLQLAGADPVMEFPLLSKKNEIVLE
                     LQTLRSKKMRLSGVETPQGRSEV"
     gene            909..>2321
                     /locus_tag="PLG78_18445"
     CDS             909..>2321
                     /locus_tag="PLG78_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE25161.1"
                     /translation="MSMEDLPEIQKIISIGKANGEITYDEINEYLPEKIVNSEWIEDV
                     FTLLNQMGIEVVEEYSSRPQEEVVKKEPPAKARKKKDAPANSSDDPIRLYLKEIGRVS
                     LISGDKEVFLARRIENGENIIEETILESSLLRANFMKLLPKIRSRKIKITDVVRANKA
                     YYMSTTELEKLEKIFFHEMETIVAEEKKLQEAQNRLKKYVEGSKKWREYRLKADESKA
                     LLLNAVRKVGVSQKEIQRISQKIKSMVFRIKEIQRHFLGIRARYGKDIKEIKAYNRFI
                     EKNENLDLIEKEMNAPIDEVREIIKDIRNNERKLRRMEQEAGSIVSDILRWGESLNRG
                     EREIAQAKKELIKANLRLVVSIAKRYANRGMHFFDLIQEGNIGLIKAVDKFEYKKGYK
                     FSTYATWWIRQAITRAISDQARTIRIPIHMIEQINKVVRETRLFLQETGREPTNEEIA
                     ERLGWPVQKVKAVKNVAREPI"
BASE COUNT          675 a          537 c          592 g          517 t
ORIGIN      
        1 ggaaactgcg tgtgcgagtg cttctacttc cagagggcat ggatgcattt gatctatctg
       61 taaaaacaga tgctgctacc attctgtctc tccttgcact ttcttttcca ggagagagat
      121 atctgatcgt agagaccatg tttccgggca ccgcagtcat tcctgcagcg gaacctggtt
      181 cagaaggtct tcttagattt ggggagcatg ttcgccgcga atacgagcgc aagagaatgg
      241 aactcgatgc gtcttctaaa agagacggcc tggccagatt ggatgagctg gtgaagacca
      301 tgccgccaga actcgggcag attttgaaac aggaaggaag cgcgatcatt ggagtatcgc
      361 ctgatcgcgc caggtctccc atgcagaaaa ggcctgctca gggtcgcact gtgatggccc
      421 ctccgctctc aaacattgca aagtgcgagc gggagatcgt tgccatcttc atgtttcatc
      481 caaccgccct cgtggattta cgcgaggaaa tggattcttt ttcctttcag gacgagccgt
      541 cagagatcat ggttcgttat ctagatacca gagctatttc tgggaatctt tggacgccgg
      601 agactgtttt ttcagaccag cttcccgatt caatcgtctc aatctttcat gggcttttac
      661 tctcacgcgc cccatcagag gaccctgttg gagtcgtgcg ggatttgctt gtagaacacc
      721 ggattcgaag tctcgaaaag gagttcgagg atctggattt gcagctggcg ggagccgatc
      781 ctgtaatgga attcccgctg ctctcaaaga agaatgagat tgtcctggaa ttgcagactt
      841 tgcggtccaa aaaaatgagg ctatctggag tggaaacacc ccagggcagg agcgaggtat
      901 aaccggccat gtcaatggaa gacctaccgg aaattcaaaa gatcatctca attggcaaag
      961 ccaacggaga gatcacatac gacgaaatca atgagtacct ccccgagaag atcgtaaact
     1021 cggagtggat tgaggacgtg tttacactcc tcaaccagat gggcatcgag gttgtggagg
     1081 aatactccag cagaccgcag gaagaagtgg tcaagaagga accacctgcc aaagcgcgaa
     1141 agaagaaaga cgcgcccgcc aattcaagcg acgatccgat tcgtctctat ctcaaagaga
     1201 ttggccgcgt ttctctgatt tcgggagaca aagaagtatt cctggcacgc cgcattgaaa
     1261 atggcgaaaa catcatagaa gaaaccatac tcgaatcgtc tttgcttcgt gcgaacttca
     1321 tgaagcttct gcccaagatt cgttctcgca aaatcaaaat cacagatgtt gtgcgcgcaa
     1381 acaaagcgta ctacatgtcc actacagaac tggaaaagct cgagaagatt ttctttcacg
     1441 agatggaaac catcgtcgca gaagagaaga agctgcagga agcgcagaat cgtctcaaga
     1501 agtacgtaga aggatccaag aaatggcgtg aataccgtct gaaagcggac gagtccaaag
     1561 cactcttgct caatgcagtg cgcaaggtgg gtgtttcaca aaaagaaatc cagcgcattt
     1621 cgcagaagat caagtccatg gtgtttcgca tcaaggagat ccagagacat ttccttggca
     1681 ttcgcgcgag atacggaaaa gacatcaaag agatcaaggc gtacaaccgt ttcattgaaa
     1741 agaatgaaaa cctggatctg attgaaaaag aaatgaacgc accgatcgat gaggtgcgcg
     1801 aaatcatcaa ggatatccgc aacaacgaaa gaaagctccg ccgtatggag caggaagcag
     1861 gttcgattgt atctgatata ctcagatggg gcgaatctct caatcgtggt gagcgcgaaa
     1921 tcgctcaggc aaaaaaagaa ctcatcaaag caaacctcag gctcgttgtt tcgattgcga
     1981 aacgttatgc gaaccgtggt atgcatttct tcgatttgat tcaggaagga aacattggcc
     2041 tgatcaaagc ggtagataaa ttcgaataca agaaaggtta taaattctcc acgtatgcta
     2101 catggtggat tcgtcaggca atcactcgtg cgatttctga ccaggcgcgc acgattcgaa
     2161 ttccaatcca catgatcgaa cagatcaaca aagtggtgcg cgagactcgt ctcttcctcc
     2221 aggaaactgg ccgggaacca accaacgaag aaattgcaga acgtcttggc tggccggtgc
     2281 agaaagtcaa agcagtcaag aatgttgcac gcgaaccaat t
//
LOCUS       DAOQJL010000594         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34785_length_2320_cov_3.154525, whole genome shotgun sequence.
ACCESSION   DAOQJL010000594 DAOQJL010000000
VERSION     DAOQJL010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34785_length_2320_cov_3.154525"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..650)
                     /locus_tag="PLG78_18450"
     CDS             complement(3..650)
                     /locus_tag="PLG78_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE25162.1"
                     /translation="MTQEEKKALITRWIVAAAFMAVFWTVIIIVTGGRYHSNVSGLHY
                     FLDMHDTSMVRAQREDTTTLENVKQALFKGADQNEVWGGPGSSMRVPPEGTVPRGYEP
                     YPYEQAEIDLAGANLKNPLPLTQTVLERGKDRFETYCAVCHGMGGQGDGTVIPRMIQP
                     PNLITGQSAGWQDGKIFHMITMGRGNMLPYAAPIRPADRWAIVHYVRLLQKKGAK"
     gene            complement(659..1330)
                     /locus_tag="PLG78_18455"
     CDS             complement(659..1330)
                     /locus_tag="PLG78_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNE25163.1"
                     /translation="MLATIDRLFQPLEGILDRVFGAILPKLHNTLDRLFEYQYNETRK
                     GVFGLFDTPEKIKKAAKAAHEKGYTGFDCFTPFPVHGLEFDMGLKRSKIPYVTFFMGM
                     CGTLTAFFLQFGVHEQISTIPYMNSYPLNIGGKPTFSWPAMVPICFELTVLFGGQCTA
                     ATLLLLSKLPRPFRKPLHKEITNDKFALWIPAHSAGYAEEDVKSFLSSIGAASVTAVD
                     EKKPF"
     gene            complement(1333..>2320)
                     /gene="nrfD"
                     /locus_tag="PLG78_18460"
     CDS             complement(1333..>2320)
                     /gene="nrfD"
                     /locus_tag="PLG78_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNE25164.1"
                     /translation="GRTWVIFWGLPIPNERGVLWPNFRSPLAWDEFAISTYFITSLVF
                     WYIGLVPDFASIRDRIDASTTGQKVRKVVYSILSFGWVGSARAWSHYEATSMILAGLS
                     MPLVFSVHSIVSFDFATSVIPGWHATIFPPYFVLGAVFSGFGMVLTLLIIMREVFRLT
                     EYITIKHLENMAKIVTLTGSLVGLAYGTEFFIAWYGGSQFEGFTFINRAFGPYWWSYW
                     IMISCNVISPQLFWFRAIRTNPVALWIISIFVNIGMWFERWVIIVTSLHRDYLPSSWD
                     MFIPTIHDYLILIGSFGMFFTFFLLFTRFFPAIAIAEIKATIKSPDAKGFGH"
BASE COUNT          607 a          547 c          618 g          548 t
ORIGIN      
        1 tattactttg cccccttctt ctggaggagc cttacgtagt gtacaattgc ccagcggtct
       61 gccggacgaa tgggagcagc atatggaagc atgttaccgc gtcccattgt gatcatgtgg
      121 aagatttttc cgtcctgcca gcctgctgac tgacctgtga tcaggttggg aggctggatc
      181 atgcgaggga ttacagttcc atcgccctga ccgcccattc catggcagac agcgcaatag
      241 gtttcaaaac gatcttttcc tctttcgagt acagtctgcg tgagaggaag aggattcttc
      301 agatttgcgc ctgccagatc gatctcggcc tgttcgtatg gatatggttc atatccgcgg
      361 ggcacggttc cttccggggg cacacgcatc gaacttccag gtccgcccca gacttcattc
      421 tggtctgcgc ccttgaagag tgcctgcttt acattttcca gagtcgttgt gtcttctctc
      481 tgcgctcgca ccatcgaggt gtcgtgcatg tccaggaaat aatgaagacc agaaacgttg
      541 ctatgataac gtccgccggt gacaatgatg atcacggtcc agaacaccgc catgaacgct
      601 gcggcaacga tccagcgggt aatgagtgcc tttttttctt cctgcgtcat acgattcctc
      661 agaatggttt cttctcatcc accgcagtta cagatgctgc tccgatggaa gaaaggaatg
      721 acttaacgtc ttcctccgca taaccggcag aatgcgcggg aatccagagt gcgaacttgt
      781 cattagtgat ttctttgtgc agaggcttac gaaatggccg gggcagtttg ctcagaagga
      841 gcagggtggc cgcggtgcat tgtccgccaa acagaactgt cagttcgaag caaatcggaa
      901 ccatggccgg ccagctgaac gttggtttgc cgccgatgtt gagaggatag gagttcatgt
      961 aaggaatcgt tgaaatctgc tcatgaacgc cgaactgcag gaaaaaagca gtcaatgttc
     1021 cgcacattcc catgaagaat gtaacatacg gaatcttgga acgcttcagt cccatatcga
     1081 attcaagtcc gtgtactgga aacggagtga agcagtcaaa gccagtgtaa cctttttcgt
     1141 gcgcagcttt ggccgctttc ttgatcttct ccggtgtatc aaataatcca aaaacacctt
     1201 tgcgtgtttc gttgtactga tactcgaaca gtcgatccag agtattgtgc agttttggaa
     1261 gaatggcccc gaacacacgg tccaggattc cttccagtgg ttgaaagaga cgatcgatgg
     1321 ttgccagcat aatcagtgtc caaatccttt cgcgtcaggc gatttgatag tcgctttgat
     1381 ttcagcgatt gcaattgccg ggaagaagcg agtgaacagt aagaagaatg tgaagaacat
     1441 tccaaatgat ccaatcagga tcaagtagtc gtggatggtc gggatgaaca tgtcccagct
     1501 agaaggaagg taatctctgt gcagagaagt cacgatgatc acccagcgtt cgaaccacat
     1561 accaatgttt acgaagatag aaatgatcca cagagccacc ggatttgtgc gaatcgcacg
     1621 gaaccagaac agctgtggtg aaatcacgtt gcaggaaatc atgatccagt aggaccacca
     1681 gtacggacca aatgcgcggt tgatgaaagt gaatccttca aattgagatc caccatacca
     1741 tgcgatgaag aattcagttc cgtaggcaag acccacgaga gagcccgtga gcgttacgat
     1801 cttcgccatg ttttcgaggt gtttgattgt gatgtattca gtgaggcgga acacttcgcg
     1861 catgatgatg agcagagtga gaaccatgcc gaatccagag aatactgcac ccagaacgaa
     1921 gtaaggaggg aagatcgtcg catgccatcc agggatgact gaggttgcaa agtcgaagga
     1981 aacgatggag tgtacggaga atacgagagg catggacaat cctgcaagga tcatcgaagt
     2041 tgcttcgtag tggctccacg ctcttgcaga tccaacccat ccaaatgaaa ggatagagta
     2101 gaccaccttg cgcactttct gacctgttgt gcttgcgtcg attctgtcgc gaatggacgc
     2161 gaagtcagga accagaccaa tgtaccagaa cacgagagat gtaatgaagt aagtagaaat
     2221 cgcaaattcg tcccaggcga gaggggagcg gaagtttggc cagagaacgc cgcgttcgtt
     2281 tggaattgga aggccccaga agatgaccca ggtacgtcct
//
LOCUS       DAOQJL010000595         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34833_length_2318_cov_4.215643, whole genome shotgun sequence.
ACCESSION   DAOQJL010000595 DAOQJL010000000
VERSION     DAOQJL010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34833_length_2318_cov_4.215643"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLG78_18465"
     CDS             complement(<1..169)
                     /locus_tag="PLG78_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25165.1"
                     /translation="MKYPHLLSPLDLGFTTLRNRVLMGSMHTGLEDMPGGYERMAAFY
                     AERAEGGVGLIV"
     gene            complement(245..1816)
                     /locus_tag="PLG78_18470"
     CDS             complement(245..1816)
                     /locus_tag="PLG78_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE25166.1"
                     /translation="MANAADLKARTEDIRTLASSIQEGGGARAKKLHESRGKLFVRDR
                     IQLLLDPGSPFLETGLFTAHQAYDFDVPSGGIVTGIGRIENTLCMIVANDATVKGGTY
                     LPLTVKKHLRAQEIALENRLPCIYLVDSGGAFLPMQDEVFPDKEHFGRIFYNQAVMSA
                     QGIPQIAVVMGSCTAGGAYIPAMSEETIIVRGNGTIFLGGPPLVKAATGEDVTAEDLG
                     GAHVHTHKSGVADHFAQNDEHALEITRRIVKHLNYRPAASAFAARFAARSPIYAPEEI
                     YGIIQKDLRKPYDIREIMARIVDGSEFHEFKENYATTIVTAFAHVFGFPVGIVANNGV
                     LFSESALKAAHFIELCSHRKIPLLFLQNITGFMVGRQYENAGIARDGAKMVTAVSNAN
                     VPKITVVVGGSYGAGNYGMCGRAYGPRFLWMWPNARISVMGAEQAANVLLTVKMEQIA
                     RERGQGLSQEEQIEFKKPILDEYGHKSHALYSSARLWDDGIIDPARTREVVGLSLAAC
                     TGSPVGEAPSSLFRM"
     gene            complement(1990..>2318)
                     /locus_tag="PLG78_18475"
     CDS             complement(1990..>2318)
                     /locus_tag="PLG78_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE25167.1"
                     /translation="KVIADIKANGTVVRPALGVQIENLSPEGKKTLGVETGVVILGVV
                     PGSGADKAGLEQKDVITKVDGTIYDDTEPLRSYILTKRVGDKVTLEIVRNKVVQQLQI
                     VLGKRQ"
BASE COUNT          556 a          626 c          571 g          565 t
ORIGIN      
        1 taacaatgag accaactccg ccttccgctc gctcagcata gaaagccgcc atgcgttcat
       61 acccgccagg catatcctca agaccggtgt gcatggatcc catcaatacg cgatttcgca
      121 gagtagtgaa acccagatcg agcggagaaa ggaggtgagg atacttcatc tggaaaaaga
      181 agaaggcgca cgcaggcgcg caagtgtttt gttttgcttt gtgggcaggg cgtcggtggc
      241 tggattacat gcgaaagagg gaagaaggtg cttcgccgac tggactgcca gtacatgctg
      301 caagagaaag accaactact tcgcgagtcc gtgcaggatc aatgatgcca tcgtcccaga
      361 gacgtgcgct cgaatagagt gcatgggact tgtgaccgta ttcgtctaga atgggtttct
      421 tgaattcaat ctgttcttcc tggcttaaac cctggccccg ctcacgcgcg atctgttcca
      481 tctttacggt gagtagtacg ttggccgcct gttctgctcc catcacagag atgcgagcat
      541 tgggccacat ccagagaaac ctgggaccat acgcacgacc acacatgccg tagttaccgg
      601 caccatacga cccgccgacc actaccgtaa tcttgggcac gtttgcattg gatacagcag
      661 tcaccatctt ggcgccatcg cgcgcaatgc ccgcattctc atactggcgc ccgaccatga
      721 agccggtgat gttctgtaga aatagaagag gaatcttacg atgcgaacac aactcaatga
      781 aatgagcggc cttcagggct gattctgaaa acagcactcc gttgtttgca acaattccaa
      841 ctggaaatcc aaacacatga gcgaatgcag tgacaatcgt agtcgcgtaa ttctctttaa
      901 attcatgaaa ctctgaccca tctacgatgc gcgccatgat ctcgcgaatg tcgtatggtt
      961 tgcgcagatc tttttgaatg atgccgtata tctcttcggg agcgtagata ggagagcgcg
     1021 cagcgaagcg tgccgcaaat gcagaagcag ccggacgata gtttaaatgt tttacaattc
     1081 tgcgagtgat ttcaagagcg tgctcatcat tttgagcaaa gtgatctgca acaccactct
     1141 tgtgagtgtg cacgtgagct ccgcccagat cttctgcggt gacatcctct cctgtggcgg
     1201 cctttacaag aggaggacca cccagaaaga tcgttccatt ccctcttaca atgattgtct
     1261 cttccgacat ggccggaata taggcgcctc ccgcagtgca gcttcccatc accactgcaa
     1321 tttgcggaat tccctgtgct gacatcacag cctggttgta gaagattcgt ccgaagtgtt
     1381 ccttgtccgg aaatacttca tcctgcatgg gcaggaacgc tcctccagaa tccaccaggt
     1441 aaatgcaggg aagacgattc tccagagcaa tctcctgcgc gcgaagatgc ttcttgactg
     1501 taagaggaag gtatgtgcca cctttgactg ttgcatcatt ggcaacaatc atgcagagag
     1561 tgttttcaat tctgccaatt ccagttacaa tgccgcctga cggcacatcg aagtcatacg
     1621 cctggtgagc ggtaaaaagc cccgtttcga ggaagggaga tccagggtcg agcagaagct
     1681 gaatgcgatc ccgcacaaag agctttcctc tagattcgtg caactttttg gcgcgagcgc
     1741 ctccgccttc ctgaatggaa gaagcaaggg tgcgaatatc ttctgtgcga gctttcagat
     1801 cagccgcatt ggccagaaaa ctctcatcct gaacaatttt actacgaaag gcgctcacat
     1861 tctttgccgg agtcaccgca agcgagcgcc tgtgcgcgag ttttgcgacg acatgatcgc
     1921 caagtttcag agctcaaacg tatgcggcaa gtgattcctg cgatcgctgc cgctctcgat
     1981 tgaaacctgt tactgtctct tacccagcac gatctgcagc tgctggacca ctttgtttcg
     2041 cacaatttcc agggtaactt tgtctccgac tctctttgtc agaatgtagg agcgaagtgg
     2101 ttccgtgtcg tcgtagatgg tgccgtccac ttttgtgatc acatcttttt gctcgagtcc
     2161 tgccttatcc gcacccgatc caggaaccac tccaagaatc accactcctg tttccacacc
     2221 gagcgtcttc tttccttccg gagatagatt ctcgatttga accccgagtg cagggcgaac
     2281 cacagttcca ttcgctttga tgtctgcgat caccttct
//
LOCUS       DAOQJL010000596         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_34863_length_2317_cov_5.007958, whole genome shotgun sequence.
ACCESSION   DAOQJL010000596 DAOQJL010000000
VERSION     DAOQJL010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34863_length_2317_cov_5.007958"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLG78_18480"
     CDS             <1..283
                     /locus_tag="PLG78_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNE25168.1"
                     /translation="GLAKRFELYWNGLELANGYEELRNKEEHLRRFKEFNDIRRKTGR
                     PEMKGDPQFLENIEKMPECSGIALGVERLMMVFLGENHIANISPFAWPK"
     gene            306..1472
                     /locus_tag="PLG78_18485"
     CDS             306..1472
                     /locus_tag="PLG78_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25169.1"
                     /translation="MRSLFTLSLLIVSLCYAMTCSSQKDKTEVTEQGTTAGQEIASVV
                     SPTNADLDKSQNWNELAKLLAGVEGPKSDSPAVNRHRALMKDFWVRIQKENMEPIAAW
                     REKNLPESAKSRNVLYPLSGADFLNAYAFFPGASDYILIALEPPGSAPHLEKMTEAQR
                     ESGLASARKAIWTLAQNNYLQSRIMHDEFNNPNVTGTLPAFLIMLGGLGHEVKNVQDI
                     GIGKTGQLAAAADAPRTKGVLIQFKDSKDGKNKRLIYLSMRLEKQTAESTSPEGIFLR
                     GIGRRNTMMKSAVYILGWESMKLPHDVILGQSDLVIQDDSGIPYKSFQDGKWNEKLYG
                     TYVRALPVGGIPNPPQQGDLAAAYAKGASPLPFAYGYGILRGKGQSNLMLFTRR"
     gene            1472..1738
                     /locus_tag="PLG78_18490"
     CDS             1472..1738
                     /locus_tag="PLG78_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNE25170.1"
                     /translation="MREIHIRQMRYDDARAKLEKEIEEAFVAGETTVDIVHGIGGGVL
                     QKMAREYVNASDILREVPQFSNPGVLRAEILSPGKDTIKSWLRR"
     gene            2130..>2317
                     /locus_tag="PLG78_18495"
     CDS             2130..>2317
                     /locus_tag="PLG78_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25171.1"
                     /translation="MSNSFGAKAKFTSKAGSLDLFRLDALEKQGVGNVSKLPYSIRVL
                     LESALRNEDSFQIHKEDV"
BASE COUNT          650 a          585 c          594 g          488 t
ORIGIN      
        1 gggtctggca aagagattcg aactctactg gaatgggctc gaactggcca acggttacga
       61 agaattgaga aacaaagaag aacacctgag acgtttcaag gaattcaacg acattcgcag
      121 gaaaacagga agacctgaaa tgaaaggcga tccgcagttc cttgaaaata ttgaaaaaat
      181 gcctgaatgt tccggaattg ccctgggcgt cgaacgtctg atgatggtat ttcttggaga
      241 gaaccacatt gcgaacatca gtccttttgc ctggcccaaa taacaacaaa ccgataatga
      301 accatatgcg ctcacttttc acactcagcc tcctcatcgt ttcactttgc tatgcgatga
      361 cctgttcttc tcagaaggac aagacagaag ttactgaaca ggggactaca gccggccagg
      421 aaattgcgtc tgttgtatct cccacgaatg cggacctgga caaatctcaa aactggaatg
      481 agcttgcaaa actcctcgcc ggagtagaag gcccaaagag cgacagcccc gctgtaaatc
      541 gccacagggc gctgatgaag gatttctggg ttcgtatcca gaaagaaaac atggagccta
      601 tcgcagcgtg gagagaaaag aatctgccgg aatctgcgaa atcgagaaat gtactctacc
      661 ctctttcggg cgctgacttc ctcaacgcgt acgctttttt tccaggtgca agtgattaca
      721 tactcattgc actcgaaccc cctggaagcg cgcctcatct ggaaaagatg acagaagcac
      781 agagagaatc aggacttgca tctgcgcgaa aagcaatctg gactctggca caaaacaact
      841 atctgcaaag cagaatcatg cacgatgagt tcaacaatcc aaacgtaact ggaactctgc
      901 ctgcttttct catcatgctc ggcggccttg gccatgaagt aaaaaatgta caggacattg
      961 gaattggaaa aacaggccag ctcgctgctg ccgcagatgc tccgcgcacg aagggagtgc
     1021 tgattcagtt caaagatagc aaagacggaa aaaacaaacg cctcatctac ttgtccatgc
     1081 gtcttgaaaa gcaaaccgca gaaagcacaa gccccgaggg aatctttctg cgcggaatcg
     1141 gaagaagaaa tacaatgatg aaatctgcag tctacatcct gggctgggaa agcatgaaac
     1201 ttcctcatga cgtgatcctg ggccagagcg atcttgtgat tcaggatgat tctggaattc
     1261 catacaaatc tttccaggac ggaaaatgga atgagaaatt atacggcact tacgtgcgcg
     1321 ctcttcctgt aggcggcatt cctaatcctc ctcagcaagg agacctggca gccgcgtacg
     1381 cgaagggggc aagccccctg cccttcgcat acggttacgg aattctgcga ggaaaggggc
     1441 agtccaacct gatgctcttt accagacgct aatgcgagag atccatatcc ggcaaatgcg
     1501 ctatgacgat gcgcgtgcga agctggagaa ggagatagaa gaagcgtttg tagcaggcga
     1561 aaccaccgtg gacattgttc acgggatcgg tggcggcgtt ctgcaaaaaa tggcccgtga
     1621 atacgtaaac gcttctgata tcttaaggga agttccgcag ttttcgaatc caggagtact
     1681 cagagcggaa atactgagcc ccggcaaaga tacaatcaaa agctggctca gacgttaggc
     1741 gacattcaac gtctggcccc ggtacctgcc tgaaataatg atcgtttgga gtggcgccgc
     1801 cagggacgct acccgttaga atcggcagac cggctgcatc cagacgatag ctagagctcg
     1861 catgggaatt ggaaacatag ggcctgacgc tgataccggg caaaaccgtg cctgcatggg
     1921 catggcgctg atttctggtg gcagtggaca ggtgtctgtt tttgccccga tggcgcgctg
     1981 gctggagcgg ctacccggcc agagcccgga aaaatccggc cctgccggcg caggggcgct
     2041 cgtttcgccg gtccggctga ccggcgaacc gcgattcgac gttctggtcc ctgcttgaca
     2101 ggcgcttttc gaaaaaagaa cgttgatcca tgagcaactc tttcggcgca aaagccaaat
     2161 tcacttccaa agcaggatca ctcgatctat tccgactcga cgcacttgaa aaacagggtg
     2221 tcggcaacgt ttccaaactt ccctactcca ttcgcgttct acttgagtct gcacttcgca
     2281 atgaagactc atttcaaatt cacaaagaag atgtaga
//
LOCUS       DAOQJL010000597         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_35051_length_2310_cov_4.265632, whole genome shotgun sequence.
ACCESSION   DAOQJL010000597 DAOQJL010000000
VERSION     DAOQJL010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35051_length_2310_cov_4.265632"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1650
                     /locus_tag="PLG78_18500"
     CDS             94..1650
                     /locus_tag="PLG78_18500"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE25172.1"
                     /translation="MQDWIPSLQDPVSYDHSVSKIELRETHISWVFLTGRFAYKIKKP
                     VDFGFLNFTTLEKRKFYCEEEVRLNRRLAPDIYLSVVPITNKDNHPFISRVALPGAEI
                     LDYAVCMAQFEPDLTLDRIESNEGLSGEQVDIIAETVADFHSRVERAALESAFGWPDQ
                     VIGPPRDNFKDTHVQGFETRLGQIAEWTEKEWARLGPVFEKRKRNGFIRECHGDMHLG
                     NIALVNDKPVIFDCIEFNEPFRWIDVQSDAAFLVMDLVERGHKDHAFRFLNRYLECTG
                     DYEGLLVLRYYLVYRAMVRAKVASLRMHQEGANVPELVLSFEHYVQMAESFANSRNPA
                     VILTCGLSGSGKTRASREFLARGMIRVRSDVERKRIFALAPEEKSGSALDQGIYTQDA
                     GKKTYDRLLYVMRVILESGYPAIVDATFLEESMRAPFFAFALELKIPLVVAHCHAEPD
                     VLRERVIRRMAKGKDASEADLNVLESQLKKGYGFSDAEKKSLVEIDTGNEEDRARAVQ
                     EVLRRAGAFE"
     gene            complement(1672..>2310)
                     /locus_tag="PLG78_18505"
     CDS             complement(1672..>2310)
                     /locus_tag="PLG78_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25173.1"
                     /translation="PVIGKTNPNAARGVTLLAPKNVKGTITGTTVNLSWTAVPGAKEY
                     SVFKAKKGGKPDFVASVSTTSYSGAFKEKPGELYFYTVRSKSEYGGESADSKGVALFV
                     NQTRSIPRERALPDQGIDRYTGTWNGRYMEKGAKPHDVVLKFASKGLEVHGTILMDNN
                     PVVFKGDYATGATYLMTDQVDMHLLDFGLLELTFKTPGKGEVTAVVEKDAEK"
BASE COUNT          550 a          558 c          607 g          595 t
ORIGIN      
        1 ctggaacttc ccaacttgtt tgcgattctg ggacccgacc aggttctttc gcgcatctct
       61 caggtggcgg gctgagtaaa gagggaatga accatgcaag attggattcc ttcccttcaa
      121 gatccagttt cctacgatca ctctgtttca aaaattgaac tgcgtgagac ccacatttcc
      181 tgggtgtttc tcacaggccg cttcgcctac aaaatcaaga aaccggtgga tttcggattt
      241 ctgaatttca ccactctcga aaagagaaag ttctattgcg aagaagaggt tcggctcaac
      301 cgcagactgg ctccggacat ttatctatct gtagttccca taaccaataa agacaatcat
      361 cctttcattt cgcgcgtagc tctgcctggc gcggagattc tggactacgc ggtatgcatg
      421 gcccagtttg agccagacct gactctcgat cgcattgaat caaacgaagg actgagcggg
      481 gagcaggtgg acatcattgc ggaaacggtg gctgatttcc acagccgcgt tgagcgcgct
      541 gcactcgaaa gtgcgtttgg ttggcctgat caggtgattg gtcctcctcg cgacaatttc
      601 aaagacacac acgttcaggg atttgaaacc aggctcggtc aaattgcaga atggacggaa
      661 aaagaatggg cgcgccttgg ccctgtgttt gaaaaacgca agcgaaatgg attcatccga
      721 gaatgtcatg gagacatgca cctgggcaat atagctctcg tgaatgataa acccgtcatc
      781 tttgactgca ttgaattcaa tgagcctttt agatggatcg atgttcaaag cgatgccgct
      841 ttccttgtga tggacctggt ggagcgtggg cacaaagatc atgcctttcg tttcttgaat
      901 cgttatctgg aatgcacggg cgactacgaa ggtcttcttg tgttgcgtta ctacctggtg
      961 tatcgcgcta tggttcgcgc gaaagtagcc agtcttcgca tgcatcagga aggcgcgaac
     1021 gttccagagc tcgtgctgag ctttgaacac tatgtgcaga tggcagaatc atttgcaaac
     1081 tctagaaatc cagcagtgat ccttacatgc ggcctatccg gttccggcaa gaccagagcc
     1141 agtcgtgagt ttctcgcacg gggaatgatt cgagttagat cggatgtgga gaggaagagg
     1201 atttttgcgc tggctcccga agaaaaaagc ggatctgctc tggatcaggg gatctatact
     1261 caggatgcag gcaaaaagac ttacgatcgt ctgctctatg ttatgcgagt gattctcgaa
     1321 tccggatatc ctgcaattgt agatgcgacc ttcctcgaag agagtatgcg cgctcctttc
     1381 tttgcgtttg ctcttgagtt gaagattccg ctcgtggtag cgcactgcca cgcagagccc
     1441 gacgttcttc gcgaaagggt tatcaggcgg atggcaaagg gaaaagatgc aagcgaagcg
     1501 gatctcaatg tcctcgagtc tcagctcaaa aaaggctacg gattcagtga tgcggaaaag
     1561 aaatctctgg tagaaataga tactggaaac gaagaggatc gcgcccgggc cgtgcaagaa
     1621 gtgctacggc gcgcgggcgc ttttgagtga gaaaacggag tctggctcgt tttatttttc
     1681 cgcgtctttc tccacgactg cggtgacttc ccctttgccg ggagtcttaa acgtaagctc
     1741 aagcagtcca aaatcaagca ggtgcatgtc tacctgatct gtcatgaggt atgtcgcgcc
     1801 tgtggcatag tcgcctttga acacaacagg gttgttatcc atcagaattg tgccgtgaac
     1861 ctcaagacct ttgcttgcaa atttcaaaac aacgtcatgc ggctttgcac ccttctccat
     1921 gtaacgtccg ttccaggttc ctgtgtatcg gtcgatgccc tgatcgggca gggcacgttc
     1981 gcgcggaatg gatctggttt gattcacaaa gagtgccact cctttagaat cagcagattc
     2041 tccgccatat tctgacttgg atcgcacagt gtagaagtag agttcgcctg gcttttcttt
     2101 gaatgctccc gaatagctgg tcgtagagac gctggctaca aagtctggct tgcctccctt
     2161 cttcgctttg aatactgaat actctttggc acctggaacc gcagtccagg aaagattcac
     2221 agtcgttccg gtgattgtgc ccttcacatt cttcggagca agcaaggtaa ctccgcgcgc
     2281 agcgttcgga tttgttttgc cgatcacagg
//
LOCUS       DAOQJL010000598         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_35349_length_2300_cov_5.015590, whole genome shotgun sequence.
ACCESSION   DAOQJL010000598 DAOQJL010000000
VERSION     DAOQJL010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35349_length_2300_cov_5.015590"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLG78_18510"
     CDS             complement(<1..642)
                     /locus_tag="PLG78_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE25174.1"
                     /translation="MSGRPLKYGVIAAAGKGTRAYPRTSYVPKPLFTFENQTLLEKNV
                     YIQLKVLKVPELYIIVGHLKEQVLSEVERIRRHYPHAKIHTAEWTGKGLASDIAALGD
                     RIDGDFSVILGDEFYMDMNHQALAKLWSQKKKAEAMIAVLKSDLLSEIRKNYSVELEG
                     TRVMKLVEKPDSPPNDLLGLGTYIFSPEYFKAFRETPPSNKSGVVELTDVIDLM"
     gene            734..2146
                     /locus_tag="PLG78_18515"
     CDS             734..2146
                     /locus_tag="PLG78_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25175.1"
                     /translation="MKATTFGELKSQGYRYRSVRTEMAENLAAKIRSKQQIFPEIHGY
                     EDTVIPQLTHAILSGHNIAILGEKGQAKSRIMRSLTTLLDDEFPVLAATEIPESPFHP
                     ITALGRQHVAAGDSAPIRWLSRDERYGERLAPGSRIADIIGDLDPSRIIAGESLGSES
                     ALHYGLLPRMNHGIFAINELPDLDYLVQVALFNVLEEADIQIRGLPIRIPLDVFVCFT
                     ANPADYSRSGKIITQLKDRIGAEIRTHYPKARSVGLAITRQEIQNLIVDPRIIVPEFM
                     ENIIEEITIQARMSPVVNQKSGVSARLSIANYETATASARRRALLLGENPGHVRLTDL
                     GNLFASSSGKIELDPYRDEAVTEFQVIQKIIDEAIREVFLEYFPLKKYEPTMDQVAKQ
                     IFEAKGLEISDTMNVESYRDILKKVPALFDFMRDRGWDSDSQTLACGVEFILEGLAVN
                     QKLSRRKLGEIVSFKSVDIY"
BASE COUNT          589 a          596 c          561 g          554 t
ORIGIN      
        1 catcaaatcg atcacatctg tgagttccac gacgcctgat ttgttagatg gaggagtctc
       61 tctgaatgct ttgaaatact ctggactaaa gatgtaagtg ccaagcccca gaagatcgtt
      121 cggtgggctg tccggttttt ccaccagctt catgactcgg gtgccctcga gttctacaga
      181 gtagttcttg cgaatctcag acaaaagatc cgatttcaaa acagcaatca tcgcctctgc
      241 tttcttcttc tggctccaca gctttgccag agcctggtgg ttcatatcca tgtagaattc
      301 gtcgccgaga atgaccgaaa aatcgccgtc aatcctgtct ccaagagcag caatgtccga
      361 cgcaaggcct tttccagtcc attctgcagt gtggatcttc gcatgcggat aatggcggcg
      421 gattcgctcc acttcggaaa gaacctgttc cttcaaatgt ccaacgatga tgtagagttc
      481 agggactttc aatactttca gttgtatgta gacgttcttc tcaagaagcg tttgattctc
      541 aaatgtgaag agaggctttg ggacgtaact cgttcttgga tacgcccggg tgcctttgcc
      601 cgcggctgca atcacaccat acttcagtgg tcggccagac attggtagag agggcgggag
      661 aacggtctcg gcgtcaaaca caacaaaatt cggttttccc tcgggagcca gcggccagga
      721 tgccgggagt catatgaaag caaccacctt cggagaactc aagtcacagg gatacagata
      781 caggtccgtt cgcacggaaa tggctgaaaa cctggccgcg aaaatccgtt ccaaacaaca
      841 gatctttcct gaaatccacg gttatgaaga tacagtcatt ccacagttga cgcacgcgat
      901 tctgtcgggt cataacattg ccatcctcgg agaaaaaggc caggcgaaat ctcgcatcat
      961 gcgctctcta accactttgc tcgatgacga attcccggtt ctcgccgcga cggaaattcc
     1021 tgagagccct tttcatccta tcaccgcact cggaagacag cacgtcgctg ccggcgactc
     1081 cgctccgatt cgctggcttt cgcgcgatga acgctacgga gagagacttg ctcctggaag
     1141 cagaatcgca gacatcattg gagacctgga tccttcgcgc atcattgccg gagaatccct
     1201 gggaagcgaa agcgctcttc actacggact tctgccaagg atgaatcacg gaatcttcgc
     1261 catcaatgaa ctgccggatc tcgattatct ggtgcaggtc gctctcttca acgttctaga
     1321 agaagccgac atccagattc gcggcctacc cattcgaatt ccactcgatg tatttgtatg
     1381 cttcaccgcg aaccctgcag actattccag atcaggaaaa atcatcacac agctgaaaga
     1441 tagaatcgga gctgaaatta gaactcacta tccgaaagcg cgttccgttg gtctggccat
     1501 cactcgtcag gaaatccaaa acctgatcgt agatccacgc atcatcgtgc cggagtttat
     1561 ggaaaacatt attgaggaga tcacaatcca ggcacgcatg tctccagtgg tgaaccagaa
     1621 gtcaggcgtt tctgcgagat tgagcattgc aaactatgaa actgcaaccg cttcggccag
     1681 aagacgtgcc cttctcctcg gagaaaatcc aggccatgta agactcacgg acctgggcaa
     1741 cctctttgct tctagctctg ggaagatcga actcgatcca taccgcgatg aagctgtgac
     1801 tgagtttcag gtgattcaga aaatcataga tgaggcaatc cgcgaagtct tcctggagta
     1861 ctttcctcta aagaagtatg agccaaccat ggatcaagtc gccaaacaaa tcttcgaagc
     1921 aaaaggcctc gaaatatcag acacaatgaa tgtggaatcg tatcgcgaca ttctgaaaaa
     1981 agttccggcc ctgtttgact ttatgcggga tcgagggtgg gactcggatt cgcagactct
     2041 tgcatgcgga gtggagttca tcctggaagg cctcgcagtg aatcagaagc tatcgagaag
     2101 aaagctcggt gaaatcgtat cgttcaaatc agtggacatc tactgagccg ggatcggctt
     2161 ttggattgat ccctgagtcg attctgacag ttttgcctgg atgaaacagc acacccgagg
     2221 cctgggcctt tacttcattg cctcgggcac ggccgccgcg gtgaactttg gatcgcgttt
     2281 tgtatatgat tcattcttca
//
LOCUS       DAOQJL010000599         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_35639_length_2290_cov_3.763311, whole genome shotgun sequence.
ACCESSION   DAOQJL010000599 DAOQJL010000000
VERSION     DAOQJL010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35639_length_2290_cov_3.763311"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..745)
                     /locus_tag="PLG78_18520"
     CDS             complement(308..745)
                     /locus_tag="PLG78_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE25176.1"
                     /translation="MALVKTASEVTNSTIGENSYFSGRFFINGSLKIDGKFEGKSLQA
                     DQLYIGVTGRVKTNVTASSVIVEGIIVGNITARNRVMLLPTARILGDISTPELIIQNG
                     VILEGRCMISNDLKHSARDYIESEYGRDNLTLERLFGTRARTA"
     gene            complement(800..1624)
                     /locus_tag="PLG78_18525"
     CDS             complement(800..1624)
                     /locus_tag="PLG78_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25177.1"
                     /translation="MSAYTNEQLKEIDKILKFIKQMEGIVRTSPSREQVERVRKELKK
                     FREKLKEMIPGIDAMTVNVDELRTKLGLTAVIPTVQETAAAKSQDDGESGDILDRFPI
                     QKASPHCTDPDVNLMATMLLVLQKDYWPAISEQHCKLDFSCANERDAIRVLLENAVRS
                     LKVLAETIEEYATADKQDFREQLLKMKNKQTRIFLFETNDVMKGVREFLKKLVDDLDH
                     GGGAVMNRDEVIKTNSRFEESNYLEGRTIADAIREVEYFTSKVIERLNLPQFKVKP"
     gene            complement(1650..>2290)
                     /locus_tag="PLG78_18530"
     CDS             complement(1650..>2290)
                     /locus_tag="PLG78_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25178.1"
                     /translation="NYFYPGKSRKLGDFRKLGAFREASEAALGWMLLGGHFVKFEKAS
                     AFCQAESGSKTNLRQWFAAANGKKSSNVEGDSTWNLDSSPGIKEGNTPVGTRGRMAAE
                     SRAGTYFQARLILARQAIASRRWMDADSYLKHTLTWHPYVLHLQAQAHIGLGELGLAA
                     ACYRKIVKRHPDRAEFCENYGRILSSMGDEAGSARALGRAAEIRSSAKRVYS"
BASE COUNT          493 a          612 c          575 g          610 t
ORIGIN      
        1 tcaagcgcgg caagcgctcg ccttccggat tctcggccgg gaattccagg cactactctt
       61 tcctgctccg gtagatctaa aatcgtaagc ccactgcctg cttcacgcaa actctccgag
      121 gaaacgacaa gggtgcgcac gtggacgcct tctgcgatgc gaacgcactc tcttgcatgt
      181 tgtgcgcctg cagtgttgat gtaaatgatg gggtgctttt ttagaatctc tggaagggag
      241 cccagaaaga tttccgggcc aatgccggca ggatcaccgc cggtgatgac gatgtgctct
      301 ttcagactta cgcagtcctc gcacgcgtgc cgaacaatct ttcaagagtg aggttgtctc
      361 tgccatactc tgattcaatg taatctcgcg cagagtgttt gagatcattg gagatcatgc
      421 aacgaccttc gaggatcaca ccgttctgga tgatgagttc cggagtagaa atatctccca
      481 ggatacgagc tgtggggaga agcatgacgc gatttcgtgc agtgatgttg cctacaatga
      541 ttccttctac gatgacagaa gatgcagtta cgtttgtttt gacccggccc gttacaccga
      601 tgtagagctg atctgcctgg agagacttgc cttcgaattt tccatcaatc ttgagtgatc
      661 cgttgatgaa gaatcggcca ctgaagtacg aattctcgcc gatcgtggag tttgtgacct
      721 cagatgctgt cttgacaagt gccattgagc cagagaagcg gactcgccgt gattcctcaa
      781 tcagaatttg ggaaaggaat cagggtttca ctttgaactg gggcaaattc agcctttcta
      841 tgaccttcga agtaaaatat tcgacttcgc ggatcgcgtc agcgatggtt cggccctcca
      901 gatagttgga ctcctcgaaa cggctgttgg tcttgatgac ttcatcccgg ttcatgacgg
      961 ctccgccccc gtggtccagg tcatccacca gcttcttaag gaattctcgc acacccttca
     1021 tcacgtcgtt cgtttcaaac aggaagatcc gggtctgctt gttcttcatc ttgaggagct
     1081 gctcgcgaaa gtcctgcttg tccgcagtgg catattcttc tatagtctca gcaagcacct
     1141 tgagacttcg cacggcgttt tccaatagca cacggatcgc gtcgcgctca ttggcgcagg
     1201 aaaaatcaag tttgcagtgc tgttcggata tggcaggcca gtagtctttc tgaagaacta
     1261 gaagcatggt tgccatcaag ttcacgtccg ggtctgtgca atgaggactc gctttctgga
     1321 taggaaaccg atccagaatg tctccgctct ctccgtcgtc ctgggatttg gccgcggctg
     1381 tttcttgaac ggttggaatg actgcggtga ggcccagttt tgtgcgaagc tcgtccacat
     1441 tgactgtcat tgcatcaatg ccgggaatca tttctttcag cttttcgcgg aacttcttga
     1501 gctccttgcg cactctctcc acctgctcgc ggcttggtga agtacgcacg atgccttcca
     1561 tctgtttgat gaatttcagg atcttatcga tctcttttag ctgttcgttt gtgtaagcgc
     1621 tcactgtgca ccttgtaagg gacaaaaccc tatgagtaaa ctctttttgc cgacgaccga
     1681 atttcggcag cgcgcccgag cgcccgggct gagcctgctt cgtctcccat cgaggaaaga
     1741 atgcgtccat agttttcgca gaattccgct cgatctggat gcctttttac aattttgcga
     1801 taacaggcgg cggccaggcc cagctctccc agtccaatgt gagcctgcgc ctgcaggtga
     1861 agtacgtaag gatgccaggt gagagtatgt ttcaaataag aatcggcatc catccatctg
     1921 cgcgaggcga tggcctgtct ggccagaatc aaccttgcct gaaagtaggt tcccgctctg
     1981 ctttctgcgg ccatcctgcc acgcgttccc actggagtat ttccttcctt gatgccaggc
     2041 gaagaatcca gattccaggt agaatcgccc tccacattgc tggatttctt tccattggct
     2101 gccgcaaacc attgccgcag attcgtctta cttccagact cagcctggca gaaggcgctt
     2161 gctttttcaa acttcacaaa atgaccgccc agaagcatcc agccaagagc cgcttccgat
     2221 gcttctctaa aagcaccgag ctttctaaag tcgcctaact tccgcgattt gcccgggtaa
     2281 aagtagttta
//
LOCUS       DAOQJL010000600         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36095_length_2275_cov_3.609910, whole genome shotgun sequence.
ACCESSION   DAOQJL010000600 DAOQJL010000000
VERSION     DAOQJL010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36095_length_2275_cov_3.609910"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2275)
                     /gene="acnA"
                     /locus_tag="PLG78_18535"
     CDS             complement(<1..>2275)
                     /gene="acnA"
                     /locus_tag="PLG78_18535"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNE25179.1"
                     /translation="KEDVENLTKWDPTKQSDREFAFKPGRVVLQDFTGVPCVVDLAAL
                     RGAMVRMGGDPARINPIVRVDLVIDHSVQVDYFGSGDALKKNMAVEFERNRERYEFLK
                     WGQGAFQNLGIVPPGIGIVHQVNLEYLARGVLEKDGVAYPDSLVGTDSHTTMINGLGV
                     LGWGVGGIEAEVVMLGQPLYMLVPQVIGFKLKGKLREGATATDLVLRVTEMLRKKGVV
                     EKFVEFYGPGLSSLALADRATIANMAPEYGATTGFFPVDAETLRYLERTGRPPELIDL
                     VERYYKEQGMFRTDDSAEPVYTDSLELDLDSVEPALAGPKRPQDRVNLSEMKKTWQGA
                     LTKPVKERGFELKPDQINAKGHITGGYQAEITHGAVVIAAITSCTNTSNPGVMVGAGL
                     LAKKAVEKGLKVKPFVKTSLAPGSRVVTDYLDRAGLSQYLDQLGFQTVGYGCTTCIGN
                     SGPVPDPVVNAINEGNLVAASVLSGNRNFEGRISPNVKANFLASPPLVVAYALAGTVD
                     IDLSKDPIGTGKDGSAIYLKDIWPAQAEVKAAVDAITPEMYRKRYDDVRQANEMWNEI
                     PVSGGAVYKFNEKSTYIQEPPFFIDMPKDPPAQASDIKGARVLAKLGDSVTTDHISPA
                     GNIAASSPAGKYLVENGVTKDDFNSYGARRGNDRVMTRGTFANIRLRNLLIPDSEGNV
                     TRYLPTNEQMSIFDAAMKYKADKTPLVVLAGAEYGTGSSRDWAAKGTYLLGVRAAIAV
                     SYERIHRSNLVGMGVLPL"
BASE COUNT          486 a          619 c          557 g          613 t
ORIGIN      
        1 gcagaggaag cactcccata cccaccaggt tggatctgtg gatcctttca tagcttacag
       61 cgatggctgc tcgcactccc aggagatacg ttcctttggc agcccagtcc cgcgaagatc
      121 cagtgccgta ttctgcgccc gcaagaacca ccagaggagt cttgtctgct ttgtatttca
      181 tcgcagcatc aaagatactc atctgttcgt ttgtgggtag atagcgcgta acgtttcctt
      241 ctgaatctgg aatgagcaaa ttgcgaaggc gaatgttcgc gaaagtcccg cgtgtcatca
      301 cgcgatcatt tccgcgtctg gccccataag aattgaaatc gtctttggtc actccattct
      361 ccaccagata tttgccggca ggagagcttg ccgcaatgtt ccccgcaggg ctgatgtggt
      421 ctgtagttac ggaatcaccc agtttcgcaa ggactcgcgc tcctttgatg tcggatgcct
      481 gagctggagg atctttaggc atgtcgatga agaacggagg ttcctggatg taggttgatt
      541 tttcgttgaa tttgtagact gctcctccgc tgaccggaat ttcattccac atttcatttg
      601 cctggcgaac gtcgtcgtac cgcttgcggt acatttcagg agtgattgcg tctacggctg
      661 ctttgacctc tgcctgggca ggccagatgt ccttgaggta gattgcagaa ccatcttttc
      721 ctgtgccgat tggatctttg gatagatcaa tgtccacagt tcctgcgaga gcatatgcta
      781 cgacaagcgg aggacttgca aggaagttcg ctttgacgtt aggcgatatt cttccctcaa
      841 agttccgatt tccagacaat acggacgcgg ccacaagatt tccttcgttg atcgcgttta
      901 ccactggatc tggcacagga ccagagtttc caatgcaagt tgtgcaacca tatccaacgg
      961 tctgaaatcc aagctgatcc aggtactgcg aaagtcccgc gcgatccaga tagtccgtca
     1021 ccacgcgaga gcccggtgcc agagatgtct tcacaaatgg ttttactttc agccctttct
     1081 ccactgcttt cttggcgagt agtccggccc ctaccatcac tcctggattg gaagtgttcg
     1141 tgcaggatgt gatggccgcg atgacgactg cgccatgtgt gatctctgcc tggtatccac
     1201 cggtgatgtg tcctttggcg ttgatctgat ccggtttgag ctcgaatcct ctttctttga
     1261 caggttttgt aagagcaccc tgccaggttt ttttcatttc gctcaggttc actctgtcct
     1321 gaggacgctt tggtcctgcg agtgctggct ccactgaatc gagatcgagc tccagactgt
     1381 ctgtatacac tggctccgct gaatcatctg tgcgaaacat tccctgctct ttgtaatatc
     1441 tttctacaag atcaatcagt tccggtggac gacctgttct ttccagatag cggagagtct
     1501 ctgcatccac cgggaaaaat ccagtggtgg ctccgtattc tggagccatg ttcgcaatgg
     1561 tcgcgcgatc cgccagagcc agagaggaaa gacccggtcc gtagaattcc acaaactttt
     1621 ctaccacacc tttcttacgg agcatttccg tgacgcgcaa gacgagatcc gttgcagtgg
     1681 ctccttcgcg tagcttgccc ttgagtttga aaccaatcac ttgaggaacc agcatataga
     1741 gaggctggcc gagcatcaca acttctgctt caatcccacc tacaccccag ccaagaactc
     1801 cgaggccgtt gatcatagta gtgtgtgagt ctgttcccac aaggctgtct ggataggcga
     1861 ctccgtcttt ttccagtact ccgcgcgcga ggtattccag gttcacctga tgaacgattc
     1921 cgattccggg aggaacaatc cccagatttt gaaacgcacc ctggccccac ttcaagaatt
     1981 catatctctc tcggtttctt tcgaactcga ctgccatgtt ttttttcaga gcgtcgcctg
     2041 atccgaagta gtccacctgc acagagtgat cgatgaccag atccactcga acgatcggat
     2101 tgatccgcgc aggatctccg cccatacgca ccatcgcgcc gcgaagagct gcgagatcta
     2161 caacgcaagg aacgcctgtg aaatcctgga gaaccactct gcctggtttg aatgcgaatt
     2221 cgcgatcgct ctgtttggtg ggatcccatt tagttaagtt ttctacatct tcttt
//
LOCUS       DAOQJL010000601         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36099_length_2275_cov_3.420270, whole genome shotgun sequence.
ACCESSION   DAOQJL010000601 DAOQJL010000000
VERSION     DAOQJL010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36099_length_2275_cov_3.420270"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLG78_18540"
     CDS             <1..261
                     /locus_tag="PLG78_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25180.1"
                     /translation="GMRQPVGFCAEAMKKVRLVTYGDNRIFFGLDKRSTNVTAEDLYT
                     RMAARYKSLHLAPENAPAWKQIVDTSILSTVTGLTGNQSPEN"
     gene            319..810
                     /locus_tag="PLG78_18545"
     CDS             319..810
                     /locus_tag="PLG78_18545"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE25181.1"
                     /translation="MGKPVVHFEIASANSTEAQTFYSKLFGWEIDANNPMKYGIVKTA
                     KGVGINGGLFQADAGKEAVVVYVAVEDTDAYLKKVVSMGGTVIHPTEVIPGMVTFALF
                     ADPFGTVMGLIKDVPPPKPAPAIAAGKKANPAAKTKAVKKAAPKKKAKKAAKKPAAKK
                     KRK"
     gene            complement(841..1392)
                     /gene="def"
                     /locus_tag="PLG78_18550"
     CDS             complement(841..1392)
                     /gene="def"
                     /locus_tag="PLG78_18550"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNE25182.1"
                     /translation="MAVRKILRIGHPELRKKSEPVPVTEIRSAEIKKLIRDMFDTMAE
                     AGGIGLAAPQVGVHKRIVIVGFDRSDRYPELKQGIERRILVNPEIEILKAPPVGFWEG
                     CLSVPDMRGYVERVRKIRLKFYDENEEFHDEVIEGFDAVVYQHECDHLDGVLYVDRLK
                     DPKKFGYNDELDLLEANRTKVIV"
     gene            complement(1428..>2275)
                     /locus_tag="PLG78_18555"
     CDS             complement(1428..>2275)
                     /locus_tag="PLG78_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE25183.1"
                     /translation="RALEWHSKGQGFDPPRLQVEVTPDSEEIISGERPPLLLIHDVFS
                     DTDAWAEQIDFLSRQYDVLNFPLSGHRGAQGSSTSSLLEDNRSRLDQIVGSVEMISII
                     AHGHSCRLAVDLASDFPDRVAQLILISPVLNPLNLMRGLPRFMPFFYEIFFLFADTLG
                     EKGPSFHRPVARLASISAPAMRQWVEEWRALPAPDLTRVQARTLILSGDRMPRVPDPP
                     VGDINENLTNSHLIRFAGMGHFLQKENAATINPLLMDFLNRPEGYLQRGLESVVGFIK
                     GLFGR"
BASE COUNT          624 a          587 c          566 g          498 t
ORIGIN      
        1 ggaatgcgtc agcctgttgg attctgcgcg gaagcaatga aaaaagtacg actggtcact
       61 tacggagaca acaggatctt tttcggactg gacaaacgtt caacaaacgt gaccgctgaa
      121 gatctgtaca caagaatggc agcgcgatac aaaagtctcc acctggcgcc tgagaacgcg
      181 ccagcctgga agcaaattgt agatacgtct atactatcca ctgtgaccgg actaaccgga
      241 aatcagtcgc ccgaaaatta gcgcagaacg cacgcgaaaa agaattgcgc ggacgaatcc
      301 cggactcacc ttccggccat gggaaaacca gttgtacatt ttgaaatcgc atccgcaaat
      361 tcaacggaag ctcagacatt ctactcaaag ctctttggct gggagatcga cgcaaacaat
      421 ccaatgaaat atggaatcgt caagaccgca aaaggtgtcg ggatcaatgg tggattattc
      481 caggcagacg caggaaaaga agctgttgta gtctatgtcg cagtggaaga cacagatgca
      541 tacctcaaga aggttgtttc tatgggtggg accgtaattc atccaacaga agtaattcca
      601 ggcatggtca ccttcgcatt gtttgccgat ccgtttggaa cggtgatggg tcttatcaaa
      661 gacgttccac cgccaaaacc tgcgcccgca atcgcggccg ggaagaaagc aaatcctgct
      721 gcaaaaacaa aagctgttaa gaaagccgcg cctaaaaaga aggcaaagaa ggcagcgaaa
      781 aagccagcgg caaaaaagaa gagaaaataa actgagaggg cgccggagat tctccggcgc
      841 tcaaactatg accttggttc tgtttgcttc tagaagatcc agctcatcgt tatatccaaa
      901 tttcttcgga tctttcaagc gatccacgta aagcacaccg tccaggtgat cgcattcgtg
      961 ctgatacacg actgcatcaa acccttctat cacctcatca tgaaactctt cattttcatc
     1021 atagaatttc agacggatct tgcgaactct ttccacgtac ccgcgcatat caggaacgga
     1081 aagacaacct tcccagaaac caacgggtgg cgcttttaag atttcaatct caggattcac
     1141 gagaatacgc cgctcaatcc cctgcttcag ctctggatat cgatcgcttc tatcaaatcc
     1201 aacaatgaca atgcgtttgt gtacacccac ctgcggtgct gccagtccaa ttccgccggc
     1261 ttctgccatg gtatcaaaca tatcacgaat gagtttcttg atctccgcgg agcgaatctc
     1321 cgtgacagga acaggttctg actttttgcg cagctccggg tgaccaattc gtaagatttt
     1381 tctgactgcc atatcccttg ttccgtggtt ctaattaaag cgaagactca ccggccgaac
     1441 agccctttga tgaaaccaac tacggactca aggccgcgtt gcaggtaacc ctcgggtcga
     1501 ttcagaaagt ccatcaacag agggttgatc gtggccgcat tctctttctg gagaaagtgc
     1561 cccatcccgg caaagcgaat caaatggctg ttcgtaagat tttcgtttat atcgcccaca
     1621 ggcggatcag gaacgcgcgg catgcgatct ccggataaga tcaaggttcg ggcctgcact
     1681 cgtgtgagat caggcgcagg tagtgctcgc cattcttcta cccattgccg catggcaggg
     1741 gcggaaatag aagcgagtct ggccactggc cgatgaaagg acggcccctt ttcgcctaga
     1801 gtgtctgcga aaaggaagaa tatttcatag aagaaaggca tgaatctggg cagaccccgc
     1861 atcaagttca gcggattgag cacgggcgaa atcaagatga gttgcgcgac ccggtccggg
     1921 aagtcactgg ccaggtccac tgccagtcta caggagtggc cgtgcgcgat gatcgatatc
     1981 atttcgacag agcccacaat ctgatccagg cgactgcgat tgtcctcaag caaggaagag
     2041 gtggatgagc cctgtgcacc ccggtggccc gaaaggggaa aattcagtac gtcgtactgc
     2101 cgcgaaagga agtcgatttg ctctgcccac gcgtccgtat ccgaaaacac gtcatggatg
     2161 agcaacaggg gtggtctttc cccggagatt atttcttctg aatctggagt cacttctacc
     2221 tggagacgag ggggatcgaa cccctgacct ttagaatgcc attctaacgc tctcc
//
LOCUS       DAOQJL010000602         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36227_length_2271_cov_3.031588, whole genome shotgun sequence.
ACCESSION   DAOQJL010000602 DAOQJL010000000
VERSION     DAOQJL010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36227_length_2271_cov_3.031588"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLG78_18560"
     CDS             complement(<1..463)
                     /locus_tag="PLG78_18560"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE25184.1"
                     /translation="MIGILGGGQLGRFLVNAAREWDIECAVLDPDPEAPAGKIAARFE
                     TGNLTDSSRVLDFARGLDLLTVEIENVSADALEEIATRIRVHPSASVLRIVQDKGIQK
                     DFYRRNNIPTAPYRLISSREELPDSESSYPLIVKQRRGGYDGRGVKKINSPA"
     gene            complement(460..1368)
                     /locus_tag="PLG78_18565"
     CDS             complement(460..1368)
                     /locus_tag="PLG78_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE25185.1"
                     /translation="MNEIKVLYDGALALERKGKPDDAIKYYTEIIKLDPGFRAAYNNL
                     GAIYARKGRQDLSIANFEKALSLGEDEAVHFNLGTEYFRREELEKARHHLKAALRIHP
                     RMLRAHIVLAYLYEKAGAPEKAEVYFKNALVVEPSNRMAALGLAVSLTGRGEEAAALQ
                     IVRSYLKEKPGDLTFENLQASLLLATGQVEQSRQEFLKLAKTSAEFTSFTDHVQKARS
                     EKDEEFSAMFEGMDLKIQSRTRRVLETRKKQAAADPTEKKQEVKDLMDLSFLYLFKGD
                     SDKALQLLFQAKKLGEETQGGEKPAS"
     gene            complement(1365..2267)
                     /gene="nadC"
                     /locus_tag="PLG78_18570"
     CDS             complement(1365..2267)
                     /gene="nadC"
                     /locus_tag="PLG78_18570"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004504655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNE25186.1"
                     /translation="MRQHFTSPVASIQPEDCDALIRLAIAEDAPAGDPTSEDIFTPQM
                     RTTARLVARESGVLCGMPLVLRLLDLFEKEAGYKIELVSSDKDGDHFERGSVLLTLKG
                     PVVGILRLERLLLNFLQYLSGIATTTSDAVRSAPPGVFILDTRKTLPGYRRLAKYAVY
                     TGGGTNHRIHLSEMAMIKDNHIAAAGGIRSAVDSIRAKHPQLPIEIEVDRVEQIPEAL
                     SLSPRVILLDNMTADTVRAAVAKIASICKTSGAMPPIVEVSGGWTPSRISELNSMELP
                     VGVSMGFLTHTTRFLDLSLEVDPA"
BASE COUNT          526 a          610 c          549 g          586 t
ORIGIN      
        1 ctgcaggcga attgattttc ttcacgccgc gcccatcgta tccacctctt ctctgtttga
       61 caatcaacgg ataagaagat tcagaatcgg gcaattcctc tctggaggaa atcaggcgat
      121 agggtgcagt tggaatgtta tttcttctat agaaatcttt ctgaatgcct ttgtcctgaa
      181 cgatccgaag aacagacgcc gacggatgaa ctcgaatgcg tgttgctatt tcttccagag
      241 catctgcact tacgttttct atctcaacgg taagtagatc caggccgcgc gcgaaatcca
      301 ggacccgcga agagtcggtg aggtttccag tttcaaatcg agctgcaatt tttccggcag
      361 gtgcttccgg gtccgggtca agcacggcac attcgatgtc ccactctcgc gctgcgttga
      421 ccaggaatct gccaagctgt cctcctccga ggattccaat catgacgcag gtttttctcc
      481 gccctgtgtt tcttcgccta atttttttgc ctgaaacaat agctgcagag ctttgtccga
      541 atcaccttta aacagataga gaaaactcaa atccatgaga tccttcactt cctgcttctt
      601 ctcagttgga tccgcggccg cctgcttctt tctggtttcc agtacgcggc gagtccggga
      661 ttgaattttc agatccatcc cttcgaacat ggcgctgaac tcttcatctt tttccgaacg
      721 cgctttctgc acatgatccg tgaaagaagt aaactccgca gatgtcttcg caagcttgag
      781 aaactcctgc ctggattgct ctacttgccc tgttgcaaga aggagagagg cctgaagatt
      841 ctcgaaagtt agatcccctg gtttttcctt tagataagag cgcacaatct ggagcgctgc
      901 tgcttcctcg cctcgaccgg tcagactcac cgccagacca agtgctgcca tgcgattgga
      961 aggttccacg accagggcat ttttgaaata gacttccgcc ttctccggcg cgcccgcttt
     1021 ctcataaaga taagccagta caatatgcgc gcgcagcatg cgaggatgaa tcctgagtgc
     1081 cgccttcagg tgatgccgag ctttctccaa ctcttcgcga cgaaagtact ccgtgcccag
     1141 attgaaatga actgcctcat cttctcccag ggacaacgcc ttttcgaaat tggcgatgga
     1201 cagatcctgt ctgccctttc gcgcgtagat cgcaccgagg ttgttgtagg ccgcgcgaaa
     1261 gccaggatct aacttgatga tttccgtgta gtacttgatg gcatcgtctg gctttccttt
     1321 cctttccaga gcaagcgcgc catcgtagag aactttgatt tcgttcatgc aggatccact
     1381 tcgagactga gatccagaaa tcgggttgta tgagtgagaa atcccatgct aactccaacc
     1441 ggaagttcca tagaattaag ttcactgatt cgagaaggcg tccatccacc cgaaacttca
     1501 acgataggcg gcattgctcc ggatgtttta cagatagacg caattttcgc aacagcggcc
     1561 cgaaccgtat ccgcagtcat gttatcgagc agaatgactc tcggagaaag tgaaagagct
     1621 tccgggattt gctccaccct atccacttcg atttcaatag gaagctgtgg atgcttcgca
     1681 cgaatcgaat cgaccgcact tcggattcca ccggcggctg caatgtgatt gtctttgatc
     1741 attgccattt cgctgagatg aattctgtga tttgtgccgc cgccggtgta gactgcgtac
     1801 tttgcgaggc gtctatatcc tggcagagtc ttcctggtat cgagtataaa aactccggga
     1861 ggagcacttc gcacagcgtc gctcgtagta gttgcaatgc cgctcaggta ttgaagaaaa
     1921 ttcaatagaa gtcgctccag tctaagaatg cccaccacgg gacctttcaa agtcaaaaga
     1981 accgaacctc tttcaaaatg atccccatcc ttgtcggagc ttacgagttc tatcttgtat
     2041 cctgcttcct tctcaaagag atccagaaga cgcaggacca gaggcatgcc gcacagcact
     2101 ccggactctc tggcaacgag gcgagcggtc gtgcgcattt gcggcgtaaa gatgtcttct
     2161 gacgtgggat caccggccgg cgcgtcttct gcgatcgcga ggcgaatcaa cgcatcgcag
     2221 tcctccggct gaatggaagc cacgggagaa gtgaaatgct gacgcatgtt t
//
LOCUS       DAOQJL010000603         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36377_length_2265_cov_3.277376, whole genome shotgun sequence.
ACCESSION   DAOQJL010000603 DAOQJL010000000
VERSION     DAOQJL010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36377_length_2265_cov_3.277376"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PLG78_18575"
     CDS             complement(<1..153)
                     /locus_tag="PLG78_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNE25187.1"
                     /translation="MRVLSGIQPSGRLHLGNYFSMMKRMIDYQANADLFCFIASYHAM
                     TTVQEGP"
     gene            238..624
                     /locus_tag="PLG78_18580"
     CDS             238..624
                     /locus_tag="PLG78_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25188.1"
                     /translation="MDLGFALLFLLAAVLIGGLIWMLFNLRDYGIDTTPSDPIRGYCP
                     ICAAGLRKGEKIRSSVVEIGNVEIQTRIKGCPYCLGEKSTRKRTCPVCKANLPVDQLI
                     LAISDPRVDKRKLAIRGCKTCYPQGF"
     gene            649..1791
                     /locus_tag="PLG78_18585"
     CDS             649..1791
                     /locus_tag="PLG78_18585"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNE25189.1"
                     /translation="MAIRFLCAPNAFKESIPAVDAALAMKRGILRVFPDAVVECIPIA
                     DGGDGTAKALSTAMPDAKIEHVTVKDPLGRMRDSQFVIGTLNKVRTAIVEMALASGLA
                     LVTEEERDVMRSSSAGTGELIRAALDAGAVQILIGLGGSATNDCGAGLLQELGAKLLD
                     SHGQSIGPGPSGLEHLTAMDLSTLDDRLGPNASVKLLVMCDVQSPLTGKTGASFVFGP
                     QKGARIEDLEHLDKLLEKTGRLLEKECNRSLIHTPGSGAAGGTAAALLAVGARLTPGF
                     QMISELLGVERAIKQSDYVFTGEGKFDSQSEEGKGPSEILKLARAHKKECFVLAGSVE
                     GDAENVFQIAGPNISRAESMRRASELIEDTARKVCEQIRTRTGTDS"
     gene            complement(1794..>2265)
                     /locus_tag="PLG78_18590"
     CDS             complement(1794..>2265)
                     /locus_tag="PLG78_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNE25190.1"
                     /translation="LSTAVGLALGRRVQKKNYNVFVCISDGECGEGMTWEAATAAVHH
                     RAPVIAFMDYNGIQIDGFTRDVCDLGDLEKKFGSFGWQVETRNGHSIPEIREALGSAA
                     ERLRKNPNVGPQMVLFKTVLGKGVSYMENVPGWHGKSPNDEEFKRAMRDLGVAV"
BASE COUNT          595 a          597 c          587 g          486 t
ORIGIN      
        1 aggaccttcc tgcacagttg tcattgcatg gtagcttgca atgaaacaaa acaaatccgc
       61 attcgcctga tagtcgatca ttcgcttcat catggaaaaa tagttcccga gatgcaaacg
      121 accgctcggc tggatgcccg acaaaactcg catgatccga attcgcgcga ccagacaggg
      181 cgaaaagcat tttgcaaatg tgctttgcac acagggcaga gaaattagtc gatgagcatg
      241 gatctcgggt tcgctctgtt attcctgctg gccgcagtcc tcattggcgg actcatctgg
      301 atgcttttca acctgcgcga ctacggaatc gacaccactc catccgatcc aattcgcggc
      361 tactgcccga tctgcgcggc tggactgaga aagggcgaaa aaatccgctc gtctgtggta
      421 gaaattggaa acgtcgaaat acaaacccgg atcaagggct gcccgtactg ccttggagaa
      481 aaatcgacca gaaaacgaac gtgcccggtc tgcaaagcaa acctgcccgt agaccagctc
      541 atccttgcca tttcggatcc aagggtagac aaacgcaagc tggccattcg cggctgcaaa
      601 acctgctatc cccagggatt ctaaattcat acgtagtact gaataataat ggcgattcga
      661 tttctatgcg cacccaatgc atttaaagaa agcatcccgg cggtagatgc agctctggcc
      721 atgaagcgcg gtattttacg agtatttccg gatgcagtcg tagaatgcat ccccatcgcg
      781 gacggcggag acggaaccgc aaaagcacta tcgactgcaa tgccagacgc aaagatcgag
      841 catgtgactg tcaaagatcc actcggtaga atgcgcgatt cacaattcgt gattggaacg
      901 ctaaacaaag taaggacagc gatcgtagag atggcgctcg catccggtct tgcactcgtt
      961 acagaagaag aaagagacgt tatgcgatct tcttctgccg gtacaggtga actcatccgc
     1021 gctgccctgg acgcgggagc cgtgcaaatc ctcatcggtc tgggtgggag tgcgacgaat
     1081 gattgcggag caggcttgct tcaggaactg ggcgcgaagc ttctggattc acacggccag
     1141 tccatcggac cgggtccgtc tggtctcgaa cacctgacgg caatggatct ttctactctg
     1201 gacgatcggc tcggaccgaa tgcgagcgtg aaactactgg ttatgtgcga tgtacagagt
     1261 cctcttaccg gaaaaacagg agcatctttt gtgtttggac ctcaaaaagg cgcgcgcata
     1321 gaagatctag aacacctgga taagctcctc gagaaaactg gcagattgct tgagaaagaa
     1381 tgcaatcgat ctttgattca cacgcctgga tcgggcgcag ccggaggaac agcagccgcg
     1441 cttcttgccg ttggggcgcg actgactcct ggcttccaga tgatttcaga attgcttggc
     1501 gtggaacgcg ccataaagca aagcgactac gttttcacgg gagaaggcaa attcgattca
     1561 caatcggaag aaggaaaagg gccatctgag atactgaaac tggcgcgcgc gcacaaaaaa
     1621 gaatgctttg ttctggcagg ttcagtagaa ggcgacgcgg agaatgtctt tcagattgcc
     1681 ggtccaaaca tcagtcgcgc agaatccatg cgaagagcat ctgaacttat agaagacaca
     1741 gcacgcaaag tgtgcgagca aatccgcaca agaaccggca cggattccta acgttagacg
     1801 gcgacgccta gatctcgcat ggcacgtttg aactcttcgt cgttgggtga cttaccatgc
     1861 cagcctggca cgttctccat ataggaaacg cctttgccca gaaccgtttt gaaaagaacc
     1921 atttgaggac caacgtttgg attcttccgc agacgctctg cagcagagcc aagtgcctca
     1981 cgaatctcag ggatgctgtg cccgtttctt gtttctacct gccagccaaa agaaccaaac
     2041 ttcttttcca gatcgcccag gtcgcagacg tcgcgagtga atccgtcaat ctggatgccg
     2101 ttgtagtcca taaacgcaat cacaggagcg cgatgatgca cggcagccgt ggccgcttcc
     2161 caggtcatac cttctccgca ttctccgtcc gaaatgcaga cgaatacatt gtagttcttt
     2221 ttttgaacac gccgacccag ggcaagaccc accgccgtcg aaagt
//
LOCUS       DAOQJL010000604         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36405_length_2264_cov_4.382073, whole genome shotgun sequence.
ACCESSION   DAOQJL010000604 DAOQJL010000000
VERSION     DAOQJL010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36405_length_2264_cov_4.382073"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLG78_18595"
     CDS             <1..339
                     /locus_tag="PLG78_18595"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE25191.1"
                     /translation="FTGPIYVILGKHSSSAADVVAMALRSSLEVNLIGVDRPGLAYFA
                     ENIPLDGGIVLKLTKGFVADERSIPLPKRGLKMDVNIDDYLPLEPPGDKPSEADPAHR
                     QLRTMLGLAR"
     gene            336..1871
                     /locus_tag="PLG78_18600"
     CDS             336..1871
                     /locus_tag="PLG78_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNE25192.1"
                     /translation="MIRFLLVCIGALWLLRCATTAEATATARKPDIDWTTRYGVLLVD
                     PDEGKYLASRKGSHLADTRPDGTAVTVPAEEAKKRRDRGYRTIQDPPFRYIDPEISTR
                     RKSNDPEVWWSGYKDEEIVEKIVRGLATAYPGYATLFDIGKTHRGRHVLALRLGSRAG
                     IEQRPSFLFDGGHHGNEPLSVEYVLDVMRTLLSSVSPVPGAPVSPVSLSYLEQRQMRE
                     YLDNFQIWCVPLVNPDGMNVFWNRSQIAGRRNGRELDGVDLNRNYPFYWNSGSRNASS
                     DRPGSTFYRGPSPGSEPETKAMMNLADRQRFVVSLSYHTFATKVLVPYTTDGAISPVP
                     MTGWRFANELVRAGRSNRPDKNYIAARNLYPVDGTDQDWLHNTYGTLAFIVEGSYQTP
                     EFATGGLASIRGMRPLSLRALSLYANGPTIQVKVLDHSGKPARVRIRRLDQALLEQEH
                     FETHPLTGTYDFILDEPGDTAIQVVFQENGKLRQILRTVHCSTGVCPVTIRLTSDTDN
                     LPW"
     gene            1876..>2264
                     /locus_tag="PLG78_18605"
     CDS             1876..>2264
                     /locus_tag="PLG78_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNE25193.1"
                     /translation="MKKSTLLEQDLTEELFQEFSQAPELGVDCEMMGLNPARDRLCLV
                     QIGKEGGPYALIQIDEQAGAPRLKSLLTNPEITKIFHFARMDCLFFKARLGIDVKGIF
                     CTKLASRLVRTYTDRHGLKELVREFTG"
BASE COUNT          572 a          598 c          584 g          510 t
ORIGIN      
        1 ttcaccggtc ctatctacgt cattctgggg aagcattcgt cctccgcagc tgatgtagtg
       61 gccatggctc ttcgctcctc ccttgaggta aatctgattg gagtggatcg tccggggcta
      121 gcgtatttcg cggagaacat acctctggat ggcggcatcg ttctgaaatt gaccaaagga
      181 tttgtcgcgg acgaacgcag catccccctt ccaaaacgcg gactgaaaat ggacgtgaac
      241 atagacgatt accttccgct cgagccaccc ggagacaagc cgtcagaagc agatccggca
      301 catcgccagc ttcgcaccat gctcggcctg gcgcgttgat aaggttcttg cttgtgtgca
      361 ttggagcgct ctggctgctc cgatgcgcta ccactgcaga ggcaacggca accgcgcgaa
      421 agcctgacat agactggacc acgcgatacg gtgtgctcct ggttgatccg gatgaaggca
      481 aatacctggc atccagaaag ggatctcatc tggcggacac gcgtccagat ggaacggcag
      541 tgactgtccc tgcagaagaa gcgaagaaga gaagagaccg cggatacaga acgattcagg
      601 atcctccatt tcggtatatc gatccagaaa tatccacaag gcgaaagagc aatgatcctg
      661 aagtctggtg gtccggctat aaagacgaag aaattgtaga gaagatagta cgcggcctgg
      721 ctacggccta tcctggctat gcaacgttat tcgacattgg aaagacacat agaggtcgtc
      781 atgttctggc actgcgcctt ggatctcgcg caggaattga acagcgacca tcttttcttt
      841 ttgatggcgg tcaccacgga aatgaaccac tctctgttga atatgttctc gatgtcatga
      901 ggactctttt gtcttcggtg agccctgtcc ccggggcacc cgtatcgcct gtttccttga
      961 gttatctgga acagcgacaa atgcgcgagt acctggacaa ctttcagatc tggtgcgtgc
     1021 cacttgtcaa tcctgatggg atgaatgtct tttggaatcg atcgcagatt gcaggaagga
     1081 gaaacggtcg cgaactggac ggcgtagatc tgaatcgcaa ttatccattt tactggaatt
     1141 caggaagccg caatgcttcg agtgacagac cgggaagcac cttttatcgc ggtccttcac
     1201 ccgggtcgga accggaaaca aaagcaatga tgaatctcgc agacagacag agatttgttg
     1261 tctctctttc gtatcatacg ttcgccacca aagtgctcgt gccttatact acggacggtg
     1321 ccatctctcc tgtgccaatg actggatggc gattcgcaaa tgagctcgtg cgtgccggaa
     1381 gaagtaatcg gccggacaag aattacattg ccgcgcgaaa tctctatccc gtggacggaa
     1441 cagatcagga ctggcttcac aatacatacg gcactctcgc cttcatcgtt gaaggttcct
     1501 atcaaactcc cgaatttgca acaggcggac tggccagcat tcgcgggatg agacccctgt
     1561 ctctgcgggc actctccctc tatgcaaacg gaccaaccat ccaggtgaag gtcctcgatc
     1621 actctggaaa accagcgcga gtccggatcc gcagactgga ccaggcattg ctcgaacagg
     1681 aacactttga aacccatccg ttgactggca catacgactt catactcgat gaaccgggcg
     1741 atacagccat tcaggtagtc tttcaagaaa acgggaagct acgccagatc ctgcgaaccg
     1801 ttcattgctc gacgggagtt tgccccgtca caatccgttt gacctcggat acagacaatc
     1861 ttccctggta gtgtcatgaa gaagtcaacg ctattggaac aggatctaac agaagagttg
     1921 ttccaggagt tttcccaggc gccggaactc ggcgttgatt gcgaaatgat gggactcaat
     1981 ccagctcgcg atcgcctctg cctggttcaa attggaaaag aaggcggacc gtacgcactc
     2041 atccagatag atgagcaagc cggagcaccc agactcaaat cgctgctcac caatccagaa
     2101 atcacaaaga tatttcactt cgcacgtatg gattgtttgt tcttcaaagc acgccttggc
     2161 attgacgtaa agggaatctt ctgcaccaaa ctggcgtcca gactggttcg cacatacacg
     2221 gatagacatg gtctcaaaga actcgttcgc gaattcactg gaga
//
LOCUS       DAOQJL010000605         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36464_length_2262_cov_5.078387, whole genome shotgun sequence.
ACCESSION   DAOQJL010000605 DAOQJL010000000
VERSION     DAOQJL010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36464_length_2262_cov_5.078387"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLG78_18610"
     CDS             <1..758
                     /locus_tag="PLG78_18610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE25194.1"
                     /translation="MDKSELGVVRTIEVLKSMGFLQTGTFTKAEYESRRILEVERNGI
                     RMIFLNYTYDTNGIAVTPGVVVNVIEKDRILKDFELARARKPDIIIVCYHFGKEYEFV
                     PNAYQKEWVDLAFQEGAQIVLGGHPHTLQPFERRVSADRFGKSAERFVIWSLGNFVSN
                     MQRRYVDGGIILNFKIVKAKEGMLIRGIDYTPVWVHTPIEKGRLKFYVLPVREHLADS
                     SMDKISLARMKQFLEDTDKILGDSEKAVKDPEK"
     gene            790..1464
                     /locus_tag="PLG78_18615"
     CDS             790..1464
                     /locus_tag="PLG78_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE25195.1"
                     /translation="MKSLANKEQAKNLARFFKTGKGDYGYGDRFLGITVPRTRMVARA
                     ALDLPVAEIQKLLRSPFHEIRLLALIIMVLKYPRKPEEQKAIYDLYMRDRAYINNWDL
                     VDLSAHHIVGPYLEHRDRSILRTLAKSKVLWDRRIAILSCFYFIKNRDFALAFELADV
                     LISDTHDLMHKAVGWMLREIGNKDPQAERAFLAERYKKMPRTMLRYAIEKFPEKERKG
                     YLAGLQ"
     gene            complement(1466..2089)
                     /locus_tag="PLG78_18620"
     CDS             complement(1466..2089)
                     /locus_tag="PLG78_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNE25196.1"
                     /translation="MSQREYKFVIAASGASGAIYGRELLRAIASSVPGESSLIVSAAA
                     LRVYNQELKGTLRTPEEYLDANLNGIASDKRVHSFRILDHADIGAKAASGSTPSDGMI
                     ICPCSMKTLAGIAHGYTSNLIERAADVCLKERRRLVVVPRETPYSLIHLRNMTQLTEA
                     GGIVLPGSPGFYQNPRTLDDLGRFIAGRILSLFDIHHSLYEGWEGED"
BASE COUNT          583 a          531 c          594 g          554 t
ORIGIN      
        1 gcatggacaa aagcgagctc ggcgtcgtgc gcacgatcga agtcttgaag tctatgggtt
       61 tcctgcagac aggcacgttc acaaaagcag aatatgaatc caggagaatt cttgaagtag
      121 agcgaaacgg aattcgaatg atcttcctca actacacata cgatacaaat gggatcgcag
      181 tgactcctgg tgtggtggtc aatgtgatcg agaaagaccg catactcaaa gactttgaac
      241 tggcgcgcgc gcgaaaaccg gacatcatca ttgtgtgtta ccactttggc aaagagtatg
      301 aatttgttcc taatgcctat cagaaggaat gggtagatct agcctttcaa gagggcgcgc
      361 aaattgttct gggaggacat ccccatactc tccagccatt cgaaaggcgc gtgagtgcgg
      421 atcgatttgg aaagagtgca gagagattcg taatctggtc tctgggcaat ttcgtctcca
      481 acatgcagcg cagatatgtg gatggcggaa tcattttgaa tttcaagatt gtgaaggcaa
      541 aagaaggaat gctcattcga ggcattgatt acactccagt ctgggttcac acacccatcg
      601 aaaagggcag gctcaagttc tacgttctac ctgttcgcga acatctggca gattccagca
      661 tggacaagat ctcccttgcc agaatgaaac aattcctgga agatacggac aagattcttg
      721 gcgactcaga aaaagcagtc aaagacccgg aaaaatagac cggttacggc tctttccctc
      781 gagagagaga tgaagtctct ggcaaataag gagcaggcaa agaaccttgc caggtttttt
      841 aagacgggca aaggggacta cggttacgga gatcgatttc tcgggatcac agtgccccgc
      901 accagaatgg tggcacgcgc tgctctcgat ctccccgtag ctgagatcca gaagctcttg
      961 cgttctcctt ttcatgaaat cagactgctg gccctgatta taatggttct aaagtatccg
     1021 cgcaagccag aagagcagaa ggccatttac gacctctaca tgagggaccg ggcttacatc
     1081 aataactggg atctcgtgga tttgtctgcc catcacattg ttggtcctta tctggagcat
     1141 cgtgatcggt ctatccttcg tactctcgca aaatctaaag tactctggga tcgcagaatt
     1201 gcgattcttt cctgcttcta tttcattaag aatcgggact ttgctcttgc gtttgagctc
     1261 gcggatgttc tgatttcaga cactcatgat ttgatgcaca aggcagttgg ctggatgctg
     1321 cgcgaaattg gaaacaagga tcctcaggcg gaaagggcgt ttctggcaga gcgttacaag
     1381 aagatgccca gaaccatgct tcgatacgcg atagaaaaat ttccagagaa ggaacgcaaa
     1441 ggctacctgg ctggcctgca atgattcaat cttctccttc ccatccttcg taaaggctgt
     1501 gatggatatc aaagagagaa agaatcctgc cggcaatgaa tcgaccaaga tcgtctaacg
     1561 tcctcggatt ttgataaaaa cccgggcttc ctggaagaac aatcccgcct gcctcagtga
     1621 gctgtgtcat attgcgcaga tgaatcaggc tgtaaggcgt ctcgcgcgga acgacaacca
     1681 ggcgtctgcg ttctttgagg catacatctg cggcgcgttc aatcagattt gaagtgtatc
     1741 cgtgcgcaat tccggccaga gtcttcatgg aacagggaca gatgatcatg ccgtctgatg
     1801 gagtagaacc gctcgcagct tttgcgccaa tgtccgcgtg atctagaatg cgaaaagagt
     1861 gaactcgttt gtccgaggct atcccattga ggtttgcatc caggtattcc tcgggcgtgc
     1921 gaagagtgcc cttcagttcc tgattgtaga cgcgaagtgc ggctgcgctg actatgagag
     1981 agctttctcc tggcacagag ctcgcgatgg ctcggagaag ttctctgccg tagatggcac
     2041 cgcttgctcc gctcgctgca atgacgaatt tgtattctct ctggctcaat tcagatatgc
     2101 ctcatcgctt tttccgtcga tgactgcttt gagcaatgca atttgtctgg cgtagcgcgc
     2161 ctgcaaatcc tgcgagagca aggaaagttt tggcgtttcg aggaatgcct ggcgaaccgg
     2221 accggatacg ggtagtatgg tggcgcagag gaaaatcaat tc
//
LOCUS       DAOQJL010000606         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36561_length_2259_cov_3.016788, whole genome shotgun sequence.
ACCESSION   DAOQJL010000606 DAOQJL010000000
VERSION     DAOQJL010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36561_length_2259_cov_3.016788"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLG78_18625"
     CDS             complement(<1..205)
                     /locus_tag="PLG78_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25197.1"
                     /translation="MRTLLLVLCAGLAAIHCKKPATVISPDEANFQKKLQESLLTAKP
                     GSVIELPAGTFHLDKGMTLSEKGV"
     gene            310..573
                     /locus_tag="PLG78_18630"
     CDS             310..573
                     /locus_tag="PLG78_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25198.1"
                     /translation="MAARRDDEHELDDITLLNVHRYPDSVPASIVASVRNSKAAQKRL
                     EELLKGMAGGRRSRVEVPVPEGQQSPAPEATSSLRDLIKKFIP"
     gene            570..1835
                     /locus_tag="PLG78_18635"
     CDS             570..1835
                     /locus_tag="PLG78_18635"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNE25199.1"
                     /translation="MKEVSIGILGAGNVGRGLIELIARDGEAIAARTGIRLKVAAVFD
                     RSYQKKSEALAGVPASDDPSIVLSNPDVSIVVELLGGLSPAGDLIRSALSAGKAVVTA
                     NKALVSSPAGPELLKLAQDQNLNFGFEAAVAGAIPIIRTLRMHCVSGEVRSIRGILNG
                     TCNYMITRMEEDGLDYDAALRLAQEKGFAEADPTFDVSGQDAAQKLAILAGIALDSYV
                     PVDRLRVEGIESIRPVDLKIARRMGWVIRLLAIARRTDAGELLRVHPAMIQEDHILAS
                     VKEERNAVFVDSTSAGPSLLVGKGAGSHPTATAVLSDVIDLARGGEVRPALFQKTEPE
                     ARADFNYRFYVRVQTHDRPGVLAEIATVFADHDISIASFHQEEGPEPVQLVIVTHAAL
                     ESAMFEAVAEMDRLRAVMLPSVVIRIEDL"
     gene            1889..>2259
                     /locus_tag="PLG78_18640"
     CDS             1889..>2259
                     /locus_tag="PLG78_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25200.1"
                     /translation="MSAPVRSVLDFEEKNGARMANLFRYALAALIAIPIVFAAQNGRE
                     LIINLVALSAYLLFTILHTVLLRRRSSSFMVVFNYLAVLYDYVLISGLIVYYSKLVSP
                     GNFAHAAKNPTLLYFLFPLALT"
BASE COUNT          475 a          576 c          608 g          600 t
ORIGIN      
        1 tgactccttt ttctgagaga gtcattccct tatccaggtg gaaagttcct gccggaagtt
       61 ctatgactga ccctggtttc gcagtcagta agctttcctg gagtttcttc tgaaaattgg
      121 cttcgtcggg tgagatgacg gtggccggtt ttttgcaatg aattgccgca aggcctgcac
      181 aaagtacgag tagcaaagtt cgcatggttc ccccctggtt ttgggcagag cctgggggcc
      241 tccggagatg gttcaagttg ttttcccgtt ttcacttgac ctgccgggtg ccgccctttc
      301 ttttcaggta tggctgcccg cagagacgac gaacatgaac tcgacgatat tacgctcctg
      361 aacgtacatc gttatccaga ttctgttcca gcatccatcg ttgcctccgt gcgcaattcc
      421 aaggcagcgc agaagagact cgaagaactt ctcaaaggga tggcaggcgg tcgccgcagt
      481 cgtgtggaag ttccggttcc agaaggacag caatcacccg caccagaagc tacttcgagc
      541 ctgcgcgatt tgataaagaa gttcattcct tgaaagaagt aagcatagga atcctcgggg
      601 cgggcaacgt tggccgcggc ctcattgaat tgatcgcgcg agacggagag gcaatcgccg
      661 cgcgcactgg cattcgcctg aaagtcgccg ctgtatttga ccgctcgtat cagaaaaagt
      721 ctgaggcact cgcgggtgtt cctgcttccg acgatccttc gattgtacta tcgaaccccg
      781 atgtatccat cgtagtcgag ctgttaggtg gtctgtcccc cgcaggcgat ttgattcgct
      841 ctgcactgag cgcgggtaag gctgttgtga ctgcaaataa agcgctcgtc tcttcgcctg
      901 caggccctga actactgaaa ctcgcgcagg atcaaaactt gaattttggt ttcgaggctg
      961 ctgtcgcggg tgccattccg atcattcgaa ctcttcgcat gcattgtgtt tccggggaag
     1021 tgcgctctat tcgcggaatt ctcaatggca catgcaacta catgatcact cgcatggaag
     1081 aggacggact ggattacgat gctgcacttc gccttgctca ggagaaggga tttgcggaag
     1141 cggatccaac ctttgatgta agcggtcagg acgcagcaca gaagcttgca atccttgcgg
     1201 gcattgctct ggattcctat gttcccgtgg atcgtcttcg cgtagaggga attgagtcga
     1261 ttcgccccgt ggatttgaag atcgcgcgac gcatgggctg ggtgattcga cttcttgcca
     1321 ttgcgcgtcg cacagacgca ggagaattgc tgcgcgttca tccggccatg attcaagagg
     1381 atcacatcct cgcttccgtc aaagaagaaa gaaatgcagt gtttgtggat tcgacttctg
     1441 ctggtccatc attgcttgta ggaaagggtg caggatcgca tccaactgcc accgcagttc
     1501 tcagtgacgt gattgatctg gcgcgcgggg gagaggtgag gcctgcgcta tttcaaaaaa
     1561 cggagccgga agcgcgggcg gatttcaatt atcgttttta tgtgcgtgtg caaacgcacg
     1621 atcgcccggg cgtacttgcg gaaatcgcaa ccgtgtttgc ggatcatgac atttccatag
     1681 catcttttca ccaggaagaa ggtccagagc cagtgcagct tgtaattgtc actcatgccg
     1741 cactcgaaag tgccatgttt gaggcagtcg cagagatgga tcgccttcgc gctgttatgc
     1801 ttccttctgt agtgattcga atcgaagact tatgattcat ccaattcgtg cacttcggga
     1861 catggtttgc ctccttcagc gcgagtctat gtccgcgccg gttcgctctg ttctggattt
     1921 cgaggagaag aatggcgcgc gcatggcaaa tctgtttcgc tatgcgctgg ccgctctcat
     1981 cgccattccc attgtatttg cagcacagaa tggtagagaa ctgatcatca acctggtcgc
     2041 actttctgca tatcttctct ttacgattct tcataccgtg ttgcttcgga gaagatcgag
     2101 ctccttcatg gtggttttta actacctggc agtcctttat gattacgtgc tcattagcgg
     2161 cctcatcgtt tactacagca aacttgtgag tccggggaat ttcgcgcatg cagcgaagaa
     2221 tccaacactg ctttattttc tctttcctct tgctctgac
//
LOCUS       DAOQJL010000607         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36735_length_2253_cov_4.730664, whole genome shotgun sequence.
ACCESSION   DAOQJL010000607 DAOQJL010000000
VERSION     DAOQJL010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36735_length_2253_cov_4.730664"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="PLG78_18645"
     CDS             complement(<1..1945)
                     /locus_tag="PLG78_18645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE25201.1"
                     /translation="MKRLLSIIRPPRFEDVEKETSSQILFALIGIGFVMLTIYGVALA
                     LVIGFEHVAARRIQTVLTTLVILAFVTLVLRQGHLRLASQITVIGVGFTLALSGFQNR
                     GVLGIGFAGYTVLTVTASLLLGMRIGLLTALAASITGGVMLYFQTNHASHDDWLNLFS
                     SWAGESTLFFLCAGIVGFSSMKMRAAFNSTREEVIQRKAAETGYREAETRLNLALQAA
                     RMGLWEWDIASGKVTWSPVTEDLFRIPRGSFTQTYEAFLALIHPEDRDALKQSITLAL
                     GPPGSFSVQHRVLAVGQTLWMECHGMLLRSSSGQPERMVGTVVDITHRKAAALVIEQS
                     EKRFRHLVDHVHVGIIVQGPESEILSVNPAALDLLGLTNSQILGKTSFDPSWNVVHED
                     GSDFPPPDHPVPRAIREKKAVQNVVMGVFRPARQDRAWLLVNAIPEISADGSVVQVLC
                     TFTDISAIKTAETALRDNERQYRGFIELSPDGVVVHQEGVLVYANRAMARILGAHGPA
                     DLIGRRVLDLVAPEMQQEIKDRIQGILSEGKIAPLREEKLVRLDGGLIDVEASGAPHT
                     WRNKPAVLVVVRDITDRKKAEARAKENELLYKSIFDAAGDGLFLADRTPRYVDVNQAG
                     LDMLGYTKEELLRMGPADLVSKED"
     gene            2043..>2253
                     /locus_tag="PLG78_18650"
     CDS             2043..>2253
                     /locus_tag="PLG78_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE25202.1"
                     /translation="MARLPLLHSSVGVPYPANPRKEQRRVRKKSLPFARPDISQRERD
                     AVDRVLRSGWLTSGPEVHAFEEEFAR"
BASE COUNT          533 a          603 c          577 g          540 t
ORIGIN      
        1 gatcttcttt agagacaagg tccgcggggc ccatgcgaag cagttcttct ttcgtgtacc
       61 cgagcatgtc caggcccgct tgatttacgt cgacgtagcg aggggttcga tccgcgagaa
      121 acagaccatc tccggcggcg tcaaaaatcg atttgtagag cagttcattt tctttcgcac
      181 gtgcttccgc tttctttcga tctgtgatat cgcgcactac tacgagcact gcaggtttat
      241 ttctccatgt gtgcggcgca ccggaggctt ctacatctat gaggcctcca tcaaggcgaa
      301 ccagtttctc ttcgcgaaga ggggcgattt tgccttctga aagtattcct tgaattctat
      361 ctttgatctc ttgctgcatt tctggcgcaa ctaaatcaag aaccctcctt ccaatcaaat
      421 cagcgggccc gtgagcaccc aggattcgcg ccatggcacg gttcgcatag acgagcacac
      481 cttcctgatg aaccactacg ccatccggtg aaagctcaat gaatccgcga tactgcctct
      541 cattgtctcg cagcgctgtc tctgccgtct tgatcgcgga gatgtctgtg aatgtgcaaa
      601 gaacctggac tacggatcca tcagcggaaa tttctggaat tgcatttaca agcaaccatg
      661 ctcgatcctg tcgtgcgggc cggaagactc ccatcacaac gttctgtacc gctttcttct
      721 cgcggattgc tctaggaact ggatgatccg gaggcggaaa gtcgctgccg tcttcgtgca
      781 ctacattcca tgatggatca aaacttgttt ttccaagaat ctgagagttg gtaagcccga
      841 gcaaatcgag agccgcagga ttcacagaaa gaatttcaga ctcgggtccc tggacgatga
      901 tcccaacgtg aacatgatcc acaaggtggc ggaatcgttt ttcactttgc tcaatcacaa
      961 gcgctgctgc cttgcgatga gtgatatcta cgacagtccc aaccattctt tctggctgac
     1021 cgctcgaaga tcgtagcagc atgccatgac attccatcca caacgtttgc ccgacggcga
     1081 gcacacggtg ttgaacagaa aaagaaccgg gcggcccgag ggccagagtg atggattgct
     1141 tcagcgcgtc gcgatcttcg gggtgaatga gtgcgagaaa ggcttcgtat gtttgagtaa
     1201 aggatccgcg cggaatgcgg aagagatctt ccgtaacggg agaccacgtt accttgcctg
     1261 aagcaatgtc ccattcccag agacccattc tggctgcctg gagcgccagg tttagtcgtg
     1321 tttccgcctc gcggtatccg gtttctgcgg cctttctctg aatgacctct tcgcgggtac
     1381 tattgaaggc agctcgcatc ttcatggagc tgaacccgac aataccagcg cagaggaaga
     1441 atagagtgct ctctccggcc cacgaagaaa aaagattcag ccagtcgtcg tgggatgcat
     1501 gattcgtttg aaagtagagc atcactcctc cggtgatgct cgcggccagt gcggtcaaaa
     1561 gaccgattct cattcccaga aggagagatg cggtcacagt gaggaccgta tagccggcaa
     1621 agccaatgcc aagtactccc cggttttgga agcccgacag tgccagtgtg aatcctactc
     1681 caatgacagt gatttgactg gcgaggcgca aatgtccctg cctgagaacc agagtcacaa
     1741 atgcgagaat taccagagta gtgagaaccg tttgaattct gcgtgcagca acgtgctcaa
     1801 agccgatcac cagggccagg gcgacgccat agatggtcag catcacgaag ccaattccga
     1861 tgagagcaaa gaggatctga gaggacgttt ctttttctac gtcctcgaag cgcggtggcc
     1921 ttataataga gagtaagcgc ttcaaataaa ttgcctttcg gccctccgtt tgtccaccct
     1981 ggaccagctc gttgcaattg caattccctt catgaatttt ccaatttgcc gatataaaat
     2041 gtatggcgcg acttccactt ttgcattctt ccgtcggtgt tccatacccc gcaaatccca
     2101 ggaaagagca gcgacgtgtg cgaaagaaga gtcttccatt tgcgcgtcca gacatttcgc
     2161 agcgcgaaag ggacgccgtc gatcgcgtac tgagatccgg atggctcact tcaggtcctg
     2221 aagttcacgc tttcgaagaa gaattcgcgc gat
//
LOCUS       DAOQJL010000608         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_36760_length_2252_cov_4.553027, whole genome shotgun sequence.
ACCESSION   DAOQJL010000608 DAOQJL010000000
VERSION     DAOQJL010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36760_length_2252_cov_4.553027"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..472)
                     /locus_tag="PLG78_18655"
     CDS             complement(272..472)
                     /locus_tag="PLG78_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25203.1"
                     /translation="MVLHGALRGWILTLFDFAIEGMLLAAFVFGILTILRRRGAPVGP
                     GVLATCLPPALYLAWILVTGES"
     gene            complement(510..956)
                     /locus_tag="PLG78_18660"
     CDS             complement(510..956)
                     /locus_tag="PLG78_18660"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNE25204.1"
                     /translation="MREELRPMMEDEGIWDVDMEVRVPGQPVNRGKGVSTHRMACGGE
                     WLIVDFKHENGFEGHAMYGFDSLKKKYTGIWIDTMRTFLVTLEGEFDRERNHLVLHGS
                     FERPEGPYKWREVTERPDKDTRIFRHFSPGPDGEFEMIHAVYKRRP"
     gene            complement(953..>2252)
                     /locus_tag="PLG78_18665"
     CDS             complement(953..>2252)
                     /locus_tag="PLG78_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE25205.1"
                     /translation="FQVFTGSMHFARNPLGYMQYVVKKYGDAVLLTAPNGGSFYLFSH
                     PAQIEEILVKKNNYFKKDIFTRRLSEILGRGLLTSDGEHWLRQRRLIQPVFSRERVQS
                     YSSFVLDYAKECAEKWEDGSTIEIHHAMMNLTLRIIVRAIFQIEAEHFSERVGAALEA
                     VMQHQAFRLKFPAPAFLTTTRLLSINRRYTRSLGELDQIISEIQKEAMSQNEEAHNSL
                     LEALASAKDESGSMDEKQIRDELMTILLAGHETTANALTWTFILLSQNPACYGRLIQE
                     VAALSSIDFQSLYSLPYAEAVIKESMRMFPPAWSIGREVVEECEIGGFRLTTGTQVGM
                     MQFITHRDSRFFENPDEFQPERWLDGKEKNLPRFAFFPFGGGQRMCVGSNLAMMEAVL
                     CLAQITKRVKLTLDPSSDVRPFASITLRPRGPVHMKVEQL"
BASE COUNT          496 a          602 c          549 g          605 t
ORIGIN      
        1 cagcgttagc cactgcttct tgaaagttct tctgaatgtt agctacagcc tggttcagag
       61 tttcttcccc tgccttcagt gcaccgattc cggcgtttac gaggccaatt gcagtgtttt
      121 cagcgttgtt catctttgac tccttcgctt aatgcgatgc accatttttt ttggtgcgcc
      181 gcacaagtca agccaaaacg gcatccagga tccagacgcg cggttttttt tagggcggcc
      241 ggccgcgacc tcagaatggc cattttctgg ctcaggactc gcctgtgacc agaatccacg
      301 cgagatagag agcaggaggc aggcaggttg ccaggacacc cgggcccacg ggagctccgc
      361 gcctgcggag gatggtcagg attccaaata caaaggcagc gaggagcata ccttctatcg
      421 caaagtcgaa taacgttaag atccatcccc ggagggctcc gtggaggacc atacctccgg
      481 cagctacgct cgaaagcaaa atgtcttctt caaggtcttc tcttgtaaac ggcgtggatc
      541 atttcgaact caccgtcggg gcccggcgaa aaatgacgaa agattcgcgt gtccttgtca
      601 ggccgttctg tcacttctct ccatttgtaa gggccttccg gtctttcaaa actgccgtgc
      661 agtactagat gattcctttc tctatcgaac tctccttcca gagtcacaag aaacgttcgc
      721 atcgtgtcta tccagatgcc cgtatatttc ttcttcagtg aatcaaagcc gtacatcgca
      781 tggccttcaa atccattttc gtgtttgaag tctacgatca accattcgcc gccgcaagcc
      841 attctatgag tgctgactcc cttgccgcga ttcaccggct gaccggggac acgcacttcc
      901 atgtccacgt cccagattcc ttcgtcttcc atcatgggcc tgagttcttc tctcatagct
      961 gttccacctt catatgaact ggtcctcttg gtctgagtgt aatcgacgcg aatggtcgaa
     1021 cgtcggatga cgggtccaga gtgagtttca ctctctttgt gatttgcgcc agacacaata
     1081 cagcttccat catggccagg ttggaaccga cgcacattct ctgtccgccg ccgaagggaa
     1141 agaaagcaaa cctggggagg ttcttttcct ttccgtccaa ccacctttct ggctgaaact
     1201 catctggatt ttcgaagaac cgggaatcac gatgagtgat gaactgcatc atccctacct
     1261 gcgttcccgt cgttaggcgg aatccgccga tttcgcattc ctccaccact tcgcgtccaa
     1321 tgctccaggc cggcgggaac atacgcatag attctttgat tacagcttcc gcgtagggca
     1381 gggaatagag cgattgaaaa tcaatcgaac tcaaagcggc aacttcctga atcaggcgcc
     1441 cgtagcatgc ggggttttgg gaaagtagaa tgaaagtcca ggtcaaagca ttggcagtgg
     1501 tttcatgacc ggcgagcagg atggtcatga gttcgtctcg gatttgcttt tcgtccatgg
     1561 atccggattc atcttttgcg ctggccaggg cttccagaag gctgttgtgt gcctcctcgt
     1621 tttgagacat ggcttccttc tggatttctg aaatgatctg gtcgagttct ccgaggctgc
     1681 gcgtataacg tctgttgatc gagaggagtc tggtagttgt cagaaacgcg ggggccggaa
     1741 acttcaaacg gaacgcctgg tgctgcatga ctgcctcgag agccgcgccc actctttctg
     1801 aaaaatgctc tgcctcaatc tggaagatcg cgcgaacaat gatgcgcaat gtcaaattca
     1861 tcattgcatg atgaatctca atggtcgatc catcttccca tttttccgcg cattcttttg
     1921 cgtaatccag gacaaagctg ctataggact ggactctttc gcgactgaac acaggctgga
     1981 tgagtcttct ctgcctgagc cagtgctctc catccgaagt aagcagtccc cgccccagga
     2041 tttcgctgag tcttcttgta aaaatgtctt tcttgaaata gttatttttt ttaactaata
     2101 tttcttctat ctgtgctggg tggctgaaca aatagaagga tcctccgttc ggtgctgtca
     2161 ataacactgc gtcgccgtat tttttaacga catattgcat atatcccagc gggttgcgtg
     2221 cgaagtgcat gcttcccgta aaaacctgga at
//
LOCUS       DAOQJL010000609         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_37403_length_2232_cov_4.997244, whole genome shotgun sequence.
ACCESSION   DAOQJL010000609 DAOQJL010000000
VERSION     DAOQJL010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37403_length_2232_cov_4.997244"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLG78_18670"
     CDS             complement(<1..756)
                     /locus_tag="PLG78_18670"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE25206.1"
                     /translation="MLSEFKLPFTIPGTTIDSISTFSILLMIAFLTASYLAPRELKRR
                     GLREEAADWSILLAVFGAIVGSKIGFVFEVWSRIWIVDESWSNTLYYILFFKNGMGEK
                     YPGQAMGLWEALFSRGGLVFYGGLALTFLLIYIYLRRSFGKENVWRYGDALMPSLAIG
                     YAIGRLGCMVSGDGCYGYGSSYDIPLLTMVYGPGAAMSSAGVRVWNTPVMEAFLSTGL
                     FAFYMLWARYQPFRPGLQTAIFLCYNGVARFLVE"
     gene            complement(740..2041)
                     /locus_tag="PLG78_18675"
     CDS             complement(740..2041)
                     /locus_tag="PLG78_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNE25207.1"
                     /translation="MEMYIARRKSFQRNMEPGDVAIFQSAPSLIRSNDVHHRFRQDSD
                     FYYLTGFGEEQSALVLTKENAILYLRKRDPERETWNGPMLGVDRAVEALAVDEARDIT
                     DFKKDIPDLLRKKDRLYYMYGRNPELDLLIMSTCDQLMRRGRTGEFGPGQILHAASLL
                     HEMRMIKSQEEIELLRECAQIASDAHIALMRSAKAGMHEYELEGIILDSFRRHNAVEG
                     YPSIVASGKNACVLHHIENNRRIESNDLILVDAGAEKHCLNSDVTRTFPVADRFNGPQ
                     KDAYEIVLAAQLRAIEKTIPGATLDEIHNAAVRDLTQGLIDLGVLSGTLDENVENKKY
                     IRYYMHRTGHWLGMDVHDVGSYVKDGVPRKLEDGMVCTVEPGLYFPDDEQTPPAFRGI
                     GIRIEDDVVVRGNNPLVLSEMIPKTISAIEALRGGIHAERV"
     gene            complement(2043..>2232)
                     /locus_tag="PLG78_18680"
     CDS             complement(2043..>2232)
                     /locus_tag="PLG78_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25208.1"
                     /translation="SAGFHKITALTGEAFRPLPEALPAGATDKLLPLLDPWNIFVGSH
                     RLYDSFLDLTRASTKDEH"
BASE COUNT          531 a          570 c          554 g          577 t
ORIGIN      
        1 ctcgaccagg aatcgcgcta ctccattgta gcacagaaag atagcagttt gcagtccagg
       61 tctaaaaggc tggtatcgcg cccagagcat atagaaggca aacaggcctg tggacaggaa
      121 cgcttccatc acaggagtgt tccagactct taccccggct gaactcatgg cagccccggg
      181 gccgtagacc atggtcaaca ggggaatgtc ataggaagac ccgtatccgt aacatccgtc
      241 cccgcttacc atacaaccga gtcttccaat tgcataacca atggcaagcg atggcatcag
      301 tgcatctccg tatctccaaa cgttttcttt gccgaagctg cgacgcagat agatatagat
      361 cagcagaaat gtaagagcaa gacctccgta gaataccagg ccgccgcggc tgaagagcgc
      421 ttcccataga cccatggcct gtcccggata tttttctccc atgccgttct taaagaatag
      481 tatgtagtac aatgtattgg accaggattc gtctacaatc cagattctac tccacacttc
      541 gaaaacaaat ccaatcttgg atccaacgat tgccccgaaa acagcaagca ggatgctcca
      601 gtctgctgcc tcctcgcgca ggcctcgccg cttgagttct cgcggggcaa gatacgacgc
      661 agtcaaaaac gcaatcatga gtagaatgct aaatgtggaa atggaatcga ttgtggttcc
      721 ggggatggta aaggggagtt taaactcgct cagcatgaat tcctccgcgc aatgcttcga
      781 ttgcggaaat tgtttttggg atcatttcgc tcaatacaag agggttgttt ccacgaacga
      841 ctacatcatc ttcgattctg attccgattc ctctgaaggc aggtggcgtt tgttcgtcgt
      901 cagggaagta aagacctggt tccacagtgc aaaccatgcc gtcttcgagt ttgcgcggaa
      961 ctccatcttt cacataggaa cccacatcgt gcacatccat gcccagccag tgtcccgtgc
     1021 gatgcatgta gtagcggatg tatttcttgt tttccacgtt ttcgtccagc gtcccagaca
     1081 gcactccaag atcaatcaat ccctgtgtca ggtcccgcac tgccgcgttg tggatttcgt
     1141 ccagagtggc tcctggaatt gttttctcaa tggcgcgcag ctgtgctgcc agaacaattt
     1201 cgtatgcatc cttttgaggc ccgttgaatc gatctgcaac cggaaatgtg cgtgtaacgt
     1261 cgctgttgag gcaatgtttt tcagcgcccg cgtccacgag gatgagatcg ttggattcaa
     1321 ttcgacgatt gttctctata tggtgaagaa cgcatgcatt cttcccggac gcaacgatgg
     1381 acggataacc ctccacagca ttgtgcctgc gaaaggaatc gagtatgatt ccttcgagct
     1441 catactcgtg catgccagct tttgcggagc gcatgagtgc aatgtgagcg tctgatgcga
     1501 tctgtgcgca ttctctcagt agttctattt cttcctggct cttgatcatc ctcatttcat
     1561 gaaggagaga cgctgcatgc agaatctgac ccggcccgaa ttcacctgtg cgtcctcttc
     1621 gcatgagttg atcgcacgtg ctcatgatga gcaagtcgag ttcaggattt cgtccataca
     1681 tgtagtagag gcggtctttt ttgcgaagaa gatcgggaat gtcttttttg aaatcggtga
     1741 tgtcgcgcgc ttcatctact gcaagagctt ccacagcgcg atctactccg agcatcggac
     1801 cgttccatgt ctctctttcc gggtcgcgtt tgcgtagata tagaattgcg ttttcttttg
     1861 tcagtacgag tgcagattgc tcttcgccaa atccagtgag gtagtagaaa tctgaatcct
     1921 gcctgaagcg atggtgcacg tcgttggagc gaatgagaga aggcgcgctc tggaagatgg
     1981 ccacgtctcc tggttccata tttctctgga acgatttgcg cctggcgata tacatttcca
     2041 tatcaatgtt catcctttgt gctggccctc gttagatcaa gaaaggaatc atacagccga
     2101 tgggaaccaa cgaatatgtt ccagggatca agaagaggca ataatttgtc ggtcgctccc
     2161 gcaggcaatg cttctggcag aggtcgaaac gcctctcctg taagtgcagt gatcttatga
     2221 aatcctgcgg at
//
LOCUS       DAOQJL010000610         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_37433_length_2231_cov_3.751379, whole genome shotgun sequence.
ACCESSION   DAOQJL010000610 DAOQJL010000000
VERSION     DAOQJL010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37433_length_2231_cov_3.751379"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLG78_18685"
     CDS             <1..369
                     /locus_tag="PLG78_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25209.1"
                     /translation="YSTGKSGILAGHWLKLGPGSFKETMSAVSSADSGWTIGLYLGSV
                     FCFWLGLVLLTGPILQLIEFIPIIGGFGEGLIKVIFGVFSFVIMGLAFLIIKYFLFIL
                     LGLALIAGFLIYKKKSAPAA"
     gene            complement(388..1032)
                     /locus_tag="PLG78_18690"
     CDS             complement(388..1032)
                     /locus_tag="PLG78_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE25210.1"
                     /translation="MERLTDRLTQQVLRDFARIGRSASFARGQTVFHDGDPYRGPYMV
                     SSGKFKICNVNEEGKEVIISIFGEGEWIAAPPVFADTPRYHAECIALEEGDLLLFPCA
                     ELRSYLLQHSEVMFEFARMIASTTLQFRERLRALTLLNVQQRLESFLSELGARKQAVA
                     LPLPKNQIASLIGSTPESVSRAFRILVDEGKLKVDGERYHLEGSGGSKADAASV"
     gene            complement(1089..1853)
                     /locus_tag="PLG78_18695"
     CDS             complement(1089..1853)
                     /locus_tag="PLG78_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE25211.1"
                     /translation="MALEVTLEKFQSDVLEKSLTVPVLVDFWAEWCGPCKMLGPVLEK
                     LEKDYGGSFLLVKVNTDLEQELAAHFRITGIPAVKLIAGGRLKDEFTGALPEAQVKAF
                     LKKNKIEPVTTTPAEAPSGADLISQADSILKKETISEGDHSVLWAAALQSFAEQKQFE
                     PYLAKIPELGSAYSDRRSALSRFVEGGDPEKLKYGRIALTDPASAMEYFLNRLQNASG
                     DARNRAKDDLVFCFHVAGNASDITNSYRRKLAAIWF"
     gene            complement(1856..>2231)
                     /locus_tag="PLG78_18700"
     CDS             complement(1856..>2231)
                     /locus_tag="PLG78_18700"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNE25212.1"
                     /translation="GFFPAQNPRYGGLLIFDEAGEESGGSLAAPVFARFVQSILPVLN
                     ESSQTFRVPDLAPIAQSAAAFEAGKIGDFRGMSAREARYLAARVYSIPVELDGSGYVK
                     SQDPPPGTPAAKVKKIKLVLEP"
BASE COUNT          522 a          610 c          541 g          558 t
ORIGIN      
        1 tattccaccg gaaaaagcgg aatcctcgct ggccactggt tgaaacttgg cccgggatct
       61 ttcaaagaaa ccatgtctgc ggtctccagt gctgattcag gctggaccat tggactgtat
      121 ctgggctccg tcttctgttt ctggcttgga ctggttctac ttacgggacc catccttcag
      181 ctgattgaat tcatcccgat cattggtgga tttggagaag gtctcatcaa ggtgatcttt
      241 ggagtgttct cttttgtgat catgggactt gcgttcctga tcattaagta cttcttgttt
      301 atcctccttg gacttgctct gattgcagga ttcttgattt acaagaagaa aagcgcaccg
      361 gccgcctaac agatgctaga cgccgcttta tacagaagcg gcgtctgctt tacttccgcc
      421 ggaaccttca aggtgatatc tctcaccatc tactttcaat tttccttcat ccacaagtat
      481 ccgaaatgcg cgactcaccg attccggagt cgaaccaatg agagacgcaa tttgattctt
      541 gggcaaaggc agagccacag cctgctttct tgcaccaagc tctgacagaa aactttcaag
      601 acgctgctgg acgttgagca aagtcagagc gcgaagtctt tcgcggaact gaagagtggt
      661 gctcgcaatc atccgtgcaa attcaaacat cacctctgag tgttgcagta gataacttcg
      721 cagctccgcg catggaaaga gcaagaggtc cccctcctca agagcgatgc actcagcatg
      781 atagcgggga gtatccgcga aaaccggagg agccgcgatc cattcgcctt cgccgaagat
      841 gcttatgatg acttctttgc cttcttcatt gacgttgcag attttgaact tgccgctaga
      901 gaccatatag ggcccgcgat aaggatcacc gtcgtgaaat acggtctggc cgcgcgcgaa
      961 ggacgccgat cgtccaatcc gggcgaaatc gcgcaaaacc tgctgcgtga ggcgatctgt
     1021 caaacgttcc atccggcata ccgttgccta ttcgccgggc agatcaatta aaatccaggc
     1081 ttccgggtct agaaccagat ggctgccagc tttcgcctgt acgagttggt gatatctgag
     1141 gcattcccgg ccacgtgaaa gcagaacacc aggtcgtcct tggccctgtt tcgcgcgtca
     1201 ccggaagcat tctgtagcct gttcagaaag tattccatgg cggaggcagg gtcggtcagg
     1261 gcgattctac catatttcaa tttttccgga tctccccctt ccacgaatcg actcaacgca
     1321 gaacgacggt ccgagtaggc actgcccagc tccgggatct tcgcaagata tggttcaaac
     1381 tgcttttgtt ccgcgaatga ttgcagtgcg gccgcccaga gaacagaatg atccccctcc
     1441 gagattgttt ccttttttag aatgctgtct gcctgagaga tcagatccgc accagacggc
     1501 gcctccgcag gagtagtggt tacaggttct attttgttct ttttcagaaa tgctttcacc
     1561 tgcgcctccg gtagcgcacc cgtgaactcg tctttcaatc ggccgcctgc aatcaacttc
     1621 actgcgggaa tgccagtgat tctaaagtgc gcggcgagct cttgctctag atccgtattc
     1681 actttcacaa gcaagaacga cccaccatag tccttttcca gtttctctaa aaccgggccg
     1741 agcattttgc acggaccgca ccactccgcc cagaaatcaa cgagcacggg gaccgtgagc
     1801 gatttctcaa gtacatcgct ctggaatttt tcaagtgtga cttctaatgc cattttcaag
     1861 gctccaaaac tagtttaatt tttttaactt tagccgccgg cgttcctggc ggaggatcct
     1921 gcgactttac atagccgcta ccatccaatt ctaccggaat cgaatacacg cgcgcggcaa
     1981 ggtatctggc ttcgcgcgca gacattccac gaaaatctcc aatcttgcct gcctcaaacg
     2041 cggctgcaga ttgcgcaatg ggtgccagat caggaacacg aaatgtttga gaactttcat
     2101 tgagaacagg aagaatgctt tgaacaaatc gcgcgaacac gggggccgcc agagaaccgc
     2161 cgctttcttc tcccgcttca tcaaaaatca aaagaccgcc gtatctgggg ttctgtgcgg
     2221 gaaagaaacc g
//
LOCUS       DAOQJL010000611         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_37608_length_2225_cov_4.915668, whole genome shotgun sequence.
ACCESSION   DAOQJL010000611 DAOQJL010000000
VERSION     DAOQJL010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37608_length_2225_cov_4.915668"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..82)
                     /locus_tag="PLG78_18705"
     tRNA            complement(11..82)
                     /locus_tag="PLG78_18705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Gln,seq:ttg)
     gene            complement(88..990)
                     /gene="ispE"
                     /locus_tag="PLG78_18710"
     CDS             complement(88..990)
                     /gene="ispE"
                     /locus_tag="PLG78_18710"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNE25213.1"
                     /translation="MNLASVSLESPAKINVGLRILGRRMDGFHELQGIFVPISFGDQV
                     QIKESEAPGFHFTLHTQNCLTGEAARRFDESTERGDIKQNTVYRALHALAPFIQDANL
                     HLEITLVKRVPAGAGLGGGSSNAGVVLKHLKQRLSLRDERAHAAAVRAGADVPFFLLD
                     GSAFVEGVGDRLRPIDFPHGQGVLCLSDFYISTPEAYRDLKRPLQGDAVQESRTSFER
                     WQEAAFSLWKDDGFLHNDFEEVAFQKHPDLARVKQALKDRGAFASLTGSGSAIYGLLP
                     EGAADLAKEMAERFPGCRFESFQF"
     gene            complement(1000..1158)
                     /gene="rpmG"
                     /locus_tag="PLG78_18715"
     CDS             complement(1000..1158)
                     /gene="rpmG"
                     /locus_tag="PLG78_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNE25214.1"
                     /translation="MREIVKLESTAGTGFFYTTTRNKKANQEKLEMKKFDPKAKKHVK
                     FVEKKLSK"
     gene            1266..2186
                     /locus_tag="PLG78_18720"
     CDS             1266..2186
                     /locus_tag="PLG78_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25215.1"
                     /translation="MPSVSTSIPRRDRSSFQANVLSRWLRFTFALIPGLLTSCLTWSV
                     TETLKEKKPEYLKFDEPPAYYAGIPYADEKRSGLIGLFEVRGTTGFYKYKVNIEFSRR
                     DEPVIWSDATVDPGPPFPYHKLTNEKIIENPFILCDSGYLLDLRPIASTDFAFPNSLI
                     QNAEPVLSARVAIEGSALYAEVKTGRGTKTYSLNVKAAKDSDGLTALRETPQVPASLQ
                     WKEVDIIELPAKERTFVFSKRGPMFVSVEPRDFRGKFLFIQSYYQRSRHIYEMWPALL
                     YPFAIAVDLVTSPIQIPLLLALKDCGRCFD"
BASE COUNT          526 a          594 c          542 g          563 t
ORIGIN      
        1 ccctgaaatg ctgggccggg aggattcgaa cctccggatg gcgggaccaa aacccgctgc
       61 cttaccgctt ggcgacggcc caatttttta gaactgaaag gactcgaatc ggcacccggg
      121 gaaccgctcc gccatctcct tcgcaagatc tgcggcccct tctggcagta gaccataaat
      181 ggccgaacct gatccagtga gagatgcaaa agcgccacga tctttcaatg cctgcttgac
      241 gcgggcgaga tctggatgct tctgaaacgc aacctcttcg aagtcgttgt gcaagaaacc
      301 atcatctttc cataaggaaa atgccgcttc ctgccacctt tcaaacgatg tccgagattc
      361 ttgaacagcg tccccctgta aaggtctttt caggtccctg tatgcttcag gtgtggaaat
      421 atagaaatcg ctgaggcaca gcacgccctg gccgtgtgga aaatcaatcg gtctgagccg
      481 atcccctact ccttctacaa aggcgctccc atcgagaaga aaaaatggaa catccgcgcc
      541 cgcgcgcact gcagctgcat gtgcgcgctc gtctcgcaga gacaatcttt gcttgagatg
      601 cttgagcaca actccagcat tcgagcttcc tccgcctaaa cctgcgcctg caggcactct
      661 tttgaccaga gtgatttcca gatggaggtt tgcgtcctgg atgaaaggag ccagtgcgtg
      721 gagtgcgcgg tatactgtgt tttgctttat gtcgccgcgc tctgtggatt catcaaagcg
      781 acgagcggcc tctccggtga ggcagttttg agtatggagt gtgaaatgaa aacctggggc
      841 ttctgattcc ttgatttgaa cctggtctcc gaatgatatg ggcacgaata tgccctggag
      901 ttcatgaaat ccgtccatcc tgcgccccag aatccgcagg ccgacgttga tcttagcagg
      961 actttctaaa gagacactcg caaggttcac gccggcggct tatttgctta gcttcttctc
     1021 aacgaattta acgtgcttct tggctttcgg atcaaacttt ttcatttcaa gcttttcttg
     1081 atttgccttt ttgttgcgcg tagtagtata gaagaatcct gtcccagcag tcgattccag
     1141 tttaacgatc tctctcataa gggcagaccg tccgaacgtt ccagagagtc aagtcaaagg
     1201 agcaccctcg ccaccggacg atggatattg ctcgacgcac tctccaactg cgcccatgaa
     1261 tgcggatgcc ttcggtctct acttcgatcc ctcggcggga tcgttcctcc ttccaggcga
     1321 acgtcctttc gcgctggctg cgattcactt tcgcactgat tccaggactc cttacgagct
     1381 gtttgacctg gagcgtcacg gaaaccctga aggaaaagaa accggagtat ctgaaattcg
     1441 atgaaccgcc agcgtattac gctggaattc cgtatgcgga cgaaaaaagg tcaggcctca
     1501 ttggactctt tgaggttcga ggtacgaccg gtttctataa gtacaaagta aatattgaat
     1561 tctcgcggag ggatgagcct gtcatctgga gcgatgccac agtggacccc gggccgccct
     1621 ttccgtatca caaacttaca aacgaaaaga tcatagagaa tccgttcatc ctctgcgact
     1681 caggctatct cctggatctg cgacccattg catccacgga ctttgcattc cccaattctc
     1741 tgatccagaa tgcggaacct gtattgtctg ctcgcgtcgc gatcgaagga agtgctctgt
     1801 atgcagaagt aaaaaccggg aggggcacga aaacatattc actgaatgtg aaggctgcaa
     1861 aggactcgga tggcctcact gccctcagag aaactcctca agttccggcc tctctgcagt
     1921 ggaaagaagt cgacatcatc gaattgcctg caaaggaaag aacgtttgta ttttcaaaac
     1981 gagggcccat gttcgtttcg gttgagcccc gggattttcg cggaaagttt ttgtttatac
     2041 aatcttacta tcaaagaagc agacacattt acgaaatgtg gcctgcactt ctttatcctt
     2101 tcgcgattgc agtggacctg gttacatctc ctattcagat cccgcttctt cttgcactca
     2161 aagattgtgg acgatgcttt gattgaatca ccttacaaat ttcgcgagaa gcgcctggta
     2221 ctttt
//
LOCUS       DAOQJL010000612         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38143_length_2208_cov_4.045982, whole genome shotgun sequence.
ACCESSION   DAOQJL010000612 DAOQJL010000000
VERSION     DAOQJL010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38143_length_2208_cov_4.045982"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /gene="rpmE"
                     /locus_tag="PLG78_18725"
     CDS             complement(<1..190)
                     /gene="rpmE"
                     /locus_tag="PLG78_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNE25216.1"
                     /translation="MKPNIHPEYKEAAVKCASCGTVYNMKSTRGNMTVDICSACHPFF
                     TGTERMIDAAGRVDRFKKK"
     gene            complement(214..1473)
                     /gene="rho"
                     /locus_tag="PLG78_18730"
                     /pseudo
     CDS             complement(214..1473)
                     /gene="rho"
                     /locus_tag="PLG78_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            complement(1765..>2208)
                     /locus_tag="PLG78_18735"
     CDS             complement(1765..>2208)
                     /locus_tag="PLG78_18735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE25217.1"
                     /translation="LLRPGTKVFLPNAKIPPPPPIWFRPAWGNLSSGFGWRPDPFSPG
                     RMNMHTGFDIVMYYRPVHAARTGVVVYAGGMGSYGMAVVIQHGDGLKTLYAHLSSVRV
                     RSGQDVQAGQEIGISGNTGLSTGPHLHFEVIQNGKPVNPARFVRF"
BASE COUNT          471 a          558 c          568 g          611 t
ORIGIN      
        1 atttcttttt gaaacgatcc acgcggcccg ccgcatcgat cattctttct gtgccggtga
       61 agaatggatg acatgcggag caaatgtcca cagtcatatt tcctcttgtg gacttcatgt
      121 tgtatacagt tccgcaagac gcgcacttca cagcagcttc tttgtattca ggatgaatat
      181 tcggtttcat aactctcctc agtagacgct ctttcaagct gtaggcacgt tcatgctgcc
      241 aagaaaatct tcgtttgttt tgctgaccct gattttttcc agcaaaagct ccatggcttc
      301 cgtgctcgac atgggactca ggacttttct gaggatgaag acgcggttga gaacgtcctt
      361 ggccagcaga agctcctctt ttcgagttcc agagcgattt atatccattg ccgggaaaat
      421 ccttttgtcg gcaagccttc tatccaggat gatttccata tttccagtgc ctttgaattc
      481 ttcaaagatc acctcgtcca tcttagaccc ggtttcaacg agtgcggttg caagaatagt
      541 caaagaaccg ccattttcta tgtttctggc tgctccgaag aagcgctttg gcttgtgaag
      601 agcgttggaa tcgacaccgc cagatagaat cttcccggaa gtaggaacca cctggttgta
      661 ggcgcgggcc agcctggtga tcgaatcaag cagaatgacc acatcgcggc catgctctac
      721 ctggcgcttt gccttctcaa tcaccatctc cgctacctga acgtggcggg atgcaggttc
      781 gtcgaaagta gaactgacca cttccccttt tacgctgcgt gccatgtctg ttacttcttc
      841 cggacgttcg tcaatcagaa gaaccatgag gatgacttct ggatgattgt gagtgatcgc
      901 gtttgcgata ttttgcatca agatcgtctt acctgtacgc ggaggagcaa cgatgagacc
      961 tctctgccct tttccaatcg gagtgagcag gtcgatgatg cgagtgtcga gcaaagacgg
     1021 attccactcc atgttcaaac gagagtctgg ataaagagga gtcaggttgt cgaaaagctg
     1081 tctgcgatga gccgcgtccg tcgtggtgcc gttgactgtg tccacgcgaa ggagtgcgaa
     1141 gaatctttcc gattcttttg gagggcgaat gtagccgttt actgtgtctc ctgtgcgaag
     1201 accgaacaga cggatctgcg aaggcgaaac gtaaatgtcg tccgggcctg gaaggtagct
     1261 gtaatcagcg ctgcgaagaa agccgtagcc ttctggcatc ttctcaagag ttccggaggc
     1321 aaggatcgat ccgttttgct ccgcttgctt ctgaatgatt ccgaaaatca gattttgttt
     1381 cttgagcccc gtcgtgtttt caacgcccag cttctctgcg agaacagtaa ggtcttcgat
     1441 tggtttgcgt tttagttctg tgatgtccag tggaggcggc tcatttcctg tgcgagcgcg
     1501 catgttgttt tcgtgttcca gttgttctgc tgctggatca aagtccgtca ttcccgggtc
     1561 ttcagatcgc gcatccatgc gatcgtaacc gccgcccggg cctcttctgt ggccgcctgg
     1621 tcttctttct ctagttcttc ctctgggcat gtgaaatatc ctgaattgtt tagttttgta
     1681 tagggttgtg gtgatgtggc caggccggaa ggcccggaaa cggggggatg gttctcgttt
     1741 tcctctcagg tgtttggtgt caaatcaaaa tctaacaaat cgcgccggat ttaccggctt
     1801 gccgttttga atgacttcaa agtgtagatg tggtcctgtc gaaagtccag tgttacctga
     1861 aattccaatc tcctgccctg cctgtacgtc ctggccgctt cgcacacgca cactgctgag
     1921 gtgagcgtat aacgtcttga gtccatctcc gtgctggatg acaacagcca ttccgtaaga
     1981 ccccattcct cccgcataca caacgactcc agtgcgcgct gcatgtaccg gtctgtaata
     2041 catcacaata tcaaatccag tgtgcatatt catgcgccct ggactgaacg gatccggtct
     2101 ccatccgaaa cctgaagaca ggttgcccca tgctggacga aaccagatgg ggggcggtgg
     2161 tggaatcttt gcattgggaa ggaatacttt tgtgcccggg cgaagcag
//
LOCUS       DAOQJL010000613         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38414_length_2201_cov_3.109506, whole genome shotgun sequence.
ACCESSION   DAOQJL010000613 DAOQJL010000000
VERSION     DAOQJL010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38414_length_2201_cov_3.109506"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLG78_18740"
     CDS             complement(<1..710)
                     /locus_tag="PLG78_18740"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNE25218.1"
                     /translation="MEASARRGIPHWSMAGLTGTLERIRACLVQSGFSIPFATVLAHV
                     TPAGKKAEPQMDLAIAASLLSALELLPQNSILLAHSPAVIGCLSLSGNILPAGDLAPM
                     LLSAARAGVRTVVVPESQKNQAVIPDLKYAFARSIQDLMKPLVELEGRAARFEIKTQD
                     SEMDYLRLPESVERAVAIASAGWHSILLMGPPGTGKNSCARAIAGLLPPPDETEAIEI
                     RSIHPEMGNTISRPVRIP"
     gene            791..1168
                     /locus_tag="PLG78_18745"
     CDS             791..1168
                     /locus_tag="PLG78_18745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HNE25219.1"
                     /translation="MHRSVLIVLLLISACTSAVIPKEKVRQLNREFEGRTYIAIVDIK
                     PEFSDSSDAKTEIIFHKNDRLRLVAENGEEWIRVKAHIVSDRPEQAIGRAIVFILRDD
                     LKKDADPLSEVRARLDKLVSVKR"
     gene            1174..>2201
                     /locus_tag="PLG78_18750"
     CDS             1174..>2201
                     /locus_tag="PLG78_18750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE25220.1"
                     /translation="MKIRQYRLDNPIMRLAVFLLVLSTALYAETETDFSQEARPMPAL
                     VPQHAESDEIDQEIKRYFEHATSGMDTDQKRLDRIFMNNEAQTRPGEDEGSFTFHELG
                     NKDETYRVKAKDTIYSISKKFGVKPQDIIKQNPDLETRALYIDEEIVIRSAKKPGPVP
                     GTSVQPTYLVQKGDNLSLIARKYRTTPAVLMKINGLGPASALKTGMSLRLPGPPLLYR
                     SMFAWPVQAPITSYFGRRYNPFLGGFPQFHKGLDLGAQLGTQFHAARDGIVIYSGRME
                     GYGNVIFVRHVGGLVTVYGHNKLNLVKQGDIVRQGQVIGQVGRTGSATGPHLHFEVRN
                     TKNEHALNP"
BASE COUNT          563 a          564 c          578 g          496 t
ORIGIN      
        1 tgtgggattc gcaccggtct tgaaattgtg tttcccattt cagggtgaat ggatctaatt
       61 tcgattgcct ccgtctcatc cggaggaggc aatagccctg caatggcacg cgcacaagaa
      121 ttctttccag tgccgggagg tcccatcaac agaatcgaat gccagcctgc agaggcgatc
      181 gccacggctc gctctactga ttctggaagg cgaaggtagt ccatttcaga atcttgcgtt
      241 ttgatttcga atcgagcggc ccggccctcc agttcgacga gaggcttcat gagatcctga
      301 atgctacgcg cgaaggcgta ttttaaatct ggaatgacgg cctggttctt ttgcgactca
      361 ggcacgacca cagtgcgaac tcctgcgcgg gccgcggaca gaagcatggg tgcgaggtct
      421 cctgcgggca ggatgttccc ggatagactc aggcagccga tcactgcggg agagtgagcg
      481 agaaggatgc tattttgtgg aagcaattcg agggcagaga gcaggcttgc tgcgatcgcc
      541 agatccattt gaggctctgc ctttttgccc gcgggagtca catgtgctag aactgtagcg
      601 aatggtatgg agaagccaga ttgaaccagg caggcccgaa tcctttctag agttccggtc
      661 aatccagcca tagaccagtg cggaattccg cgacgtgcag aagcttccac ggaagcgaca
      721 gcggaggccg gcccggatgt cagggtaagg gtgcttgcat atttcatcac ctttcgatgg
      781 tatggcaact atgcatcgtt ccgtcctgat tgttctactc cttatcagtg cttgcacctc
      841 tgctgtgatt ccaaaggaaa aagtccggca gctcaacaga gaattcgagg gtcgcaccta
      901 cattgcgata gtagacataa agcctgagtt ttcggacagt tctgatgcga agactgagat
      961 cattttccat aagaacgatc gcctgcgcct ggtcgcagaa aacggcgaag agtggatccg
     1021 ggtcaaggca cacatcgttt ctgaccggcc agagcaggcc attggccgcg ccattgtatt
     1081 cattttgcgg gatgatttga aaaaggacgc cgatcctctc tccgaggtgc gggcccgact
     1141 cgataaactg gtgagcgtaa aacgatgaaa catgtgaaga tcagacaata ccggctcgat
     1201 aatccaatca tgcgtctggc tgtgtttctg cttgtgctct ccacagcgct ctatgccgaa
     1261 actgaaaccg atttcagtca ggaagcccgt cctatgccgg ctctcgtgcc gcaacatgcg
     1321 gaatccgacg aaattgatca agaaatcaaa cgctactttg aacacgcaac ttccgggatg
     1381 gacacagacc agaagcgcct ggaccgaatc ttcatgaaca atgaagctca aacaagacca
     1441 ggcgaagacg aaggctcatt tacatttcac gaattgggca ataaagacga aacctatcgc
     1501 gtcaaagcga aagatactat ctatagcatt tcgaagaagt tcggcgtcaa accacaggac
     1561 atcatcaagc agaatccaga tctagaaacg cgtgcacttt acattgacga agaaatcgtg
     1621 atccgatctg cgaaaaagcc aggtccagtg cccggaacat ccgtgcagcc aacctacctc
     1681 gttcagaaag gggacaatct ctccctcatt gcacgcaagt acagaaccac tcccgcagtt
     1741 ctcatgaaaa taaatggact gggacctgcg agtgctctta agactggaat gagtttgaga
     1801 cttcctggtc ctcctctact ttaccgctcg atgttcgcct ggcctgtgca ggctccgatc
     1861 acttcctatt ttggaagacg ttacaatcct tttcttggcg ggtttcctca atttcataaa
     1921 ggactcgatc tgggtgcgca actgggcacg cagttccacg cagcgcgcga cggaatcgtc
     1981 atttactcag gtagaatgga agggtacgga aacgttatct tcgttcgcca cgtgggtgga
     2041 cttgtaaccg tctatggtca caacaagctg aacctggtca aacaaggaga cattgtgcgc
     2101 cagggtcagg tgatcgggca agtagggcgc accggatctg caaccggacc gcatttacat
     2161 tttgaagtta gaaacaccaa aaacgaacac gctttaaatc c
//
LOCUS       DAOQJL010000614         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38441_length_2200_cov_4.428904, whole genome shotgun sequence.
ACCESSION   DAOQJL010000614 DAOQJL010000000
VERSION     DAOQJL010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38441_length_2200_cov_4.428904"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLG78_18755"
     CDS             complement(<1..1718)
                     /locus_tag="PLG78_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNE25221.1"
                     /translation="MAFPEFSSSRKRASVELEILDFWKQKGVFESQVKATASGPKFVF
                     YEGPPTANGKPGIHHVLSRVFKDIYVRYYGQRGFQIPRQAGWDCHGLPVEREVEKELG
                     ITSKSEIEGKVGLETFNRLCRENVQKYISDWNTFSERLGFWIDLKNAYYTMDNSYIEA
                     VWGLLKNLWDKKLIEKSYKVVPVDPVMGSTLSDAEVAQGYKTVEDPSLTVRFRIADDK
                     YKDTSFLVWTTTPWTLPSNVALAIAPNESYALVEAVREDGSEKIICAEVLLESVLSNT
                     KVTSHSIIKKMKGSDLAGVRYERLFPWLPTEGDKAFRVYTADFVTMDTGTGIVHIAPA
                     YGADDLVVGQTHDLPVLYAVGLDGKFVSGTPQAGVFFKDADKDLVRDLKERGLVWKSE
                     RYKHEYPFGWRTGAPLLYFAKDAWYIRTTRVREHLVKNNDSINWVPDHVKQGRFGNWL
                     ENNRDWALSRERYWGTPLPVWTDGENFKIIGSIAELEKLTGQKLTELDLHRPFIDRIT
                     WKEGAAKTAKAAGTAAKSQKKSGKSSKAATSGEEAKEWKRVPEVIDCWFDSGAMPYAQ
                     WGTSPAG"
     gene            1844..>2200
                     /locus_tag="PLG78_18760"
     CDS             1844..>2200
                     /locus_tag="PLG78_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25222.1"
                     /translation="MSTETAQDPVISRLYTHLNEEKVTRDLSSVQLQKIKTLDELMQE
                     IQASGKKDELIHTLEENLKDNADSMISRYLLGRIHFHKGDERGTEHLRSLLEDFRTFA
                     RWPIVDHIADGILAHDD"
BASE COUNT          549 a          564 c          514 g          573 t
ORIGIN      
        1 cctgcaggcg aagttcccca ctgcgcgtaa ggcatggctc cagaatcaaa ccagcaatca
       61 atcacctctg gaactctttt ccattccttt gcttcttcgc ctgaagtggc ggctttcgaa
      121 ctcttgcctg atttcttttg cgactttgcc gcagttccag ctgcctttgc cgtttttgct
      181 gcaccttcct tccacgttat gcgatcaatg aaaggtctgt gcaaatccag ttcagtcaat
      241 ttctgaccgg tgagcttctc cagctctgca atgctaccga tgattttaaa attctccccg
      301 tccgtccaga ccggaagagg tgttccccag tagcgttcgc gcgaaagggc ccagtctcta
      361 ttgttttcaa gccagtttcc aaatcgaccc tgcttgacat gatcggggac ccagttgata
      421 gaatcgttgt tttttaccag atgttcacgc acgcgcgtag tgcgaatgta ccaggcgtcc
      481 tttgcaaagt acagaagtgg ggcgcctgtt ctccagccaa aaggatattc gtgtttgtat
      541 ctttcagact tccagacaag gccgcgttct ttgagatcgc gtacaaggtc cttgtcagca
      601 tccttgaaaa aaactccagc ctgaggtgtg ccggaaacaa acttaccatc caggcccacc
      661 gcatacaaca ccggaagatc atgagtctgg ccgacgacca ggtcatctgc accataggca
      721 ggagcaatgt gaacaatgcc ggtacctgta tccatggtta cgaaatccgc ggtgtagacg
      781 cgaaatgctt tgtccccttc ggttggcagc cacgggaaaa gacgttcgta gcgaacgccc
      841 gcaagatcgg atcctttcat ttttttaatg atggaatgag aggtaacctt tgtattagag
      901 agaacagatt cgagcaatac ttcagcacag atgatttttt cggaaccgtc ttcgcgtact
      961 gcttctacca gcgcgtaaga ttcattcgga gcgatcgcaa gtgccacgtt gcttggaaga
     1021 gtccagggag ttgtggtcca gacgagaaaa gaagtatctt tgtatttatc atctgctatg
     1081 cgaaagcgaa ctgtaagaga cggatcttct acagttttgt aaccctgtgc cacctctgcg
     1141 tctgaaagag tggaacccat cacaggatcg acaggaacta ctttgtagct tttttcgatg
     1201 agtttcttat cccacaggtt cttgagcaag ccccagacag cttcgatgta cgaattgtcc
     1261 atcgtgtagt atgcattttt caaatcgatc cagaagccga gtctctcgct aaacgtgttc
     1321 cagtctgaga tgtacttctg aacgttctcg cggcaaagac gattgaaggt ttcgagtcca
     1381 accttccctt cgatttcaga tttagaagtg atccctaact ctttttctac ttctctctcc
     1441 acgggaagac cgtggcaatc ccaacctgcc tgacgcggga tttgaaaacc tctctgtcca
     1501 taatagcgaa cgtaaatatc tttgaagact cgactcagta catggtgaat tcctggcttg
     1561 ccgtttgccg tgggcggacc ttcgtagaaa acgaatttgg gtccggatgc agttgctttg
     1621 acctgagact caaaaacacc cttttgcttc caaaaatcca gaatctcaag ctcaacggaa
     1681 gcacgctttc tgctggatga gaactctggg aacgccattc gccagatttg gcccggcggg
     1741 gtcttttttc aaacatatcg tagttagcga aagggtttga cctggagggt aaatccgggg
     1801 atgttggatt ctaactccat tttatagaac aaagcagcat cggatgagca cagaaacagc
     1861 acaggacccc gtcatatcca ggttatacac gcacctcaat gaggagaagg tcacccgcga
     1921 cctttctagc gtccagcttc agaagatcaa gaccctagac gaattgatgc aggaaatcca
     1981 ggcctctggc aagaaggatg aactgatcca cactctggaa gaaaatctga aggacaacgc
     2041 ggactctatg atttcgcgct acctcctggg tagaatccat tttcacaagg gagacgaacg
     2101 cggcaccgaa caccttcgtt ctcttctaga ggatttcaga acctttgcac gctggcctat
     2161 cgtagatcac attgcggatg gaattctggc ccatgacgac
//
LOCUS       DAOQJL010000615         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38572_length_2196_cov_3.931340, whole genome shotgun sequence.
ACCESSION   DAOQJL010000615 DAOQJL010000000
VERSION     DAOQJL010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38572_length_2196_cov_3.931340"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..722)
                     /locus_tag="PLG78_18765"
     CDS             complement(207..722)
                     /locus_tag="PLG78_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25223.1"
                     /translation="MRVILLLLAFFAGCSRNPSGSWTMTSEDAGLNGTIELTGQKFFR
                     VRVKSKDVDTAGAGLATAGRVAVCLGTGCRVRVLIEKFGNWRGMATDGLRTTDESWEK
                     SGQDLIGPAGKKPLGGNAMPGPNQFIAELHDQVVLADDAEGLILLDLARDSGRGEIIR
                     PSGKKSITLRR"
     gene            complement(771..>2196)
                     /locus_tag="PLG78_18770"
     CDS             complement(771..>2196)
                     /locus_tag="PLG78_18770"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNE25224.1"
                     /translation="CLLKTINTNESQALLFERIENAILPFIAVFFLFFAQRFRPILPD
                     WFVWVFTIADAMLSLIILFYPHAYNSELGAVKTFPALGVTFYETKQPVWVFMFIALNL
                     IGMAGIIVRYLQLGLFRQIESRILVVSLVLFFLMGANDMAVSLEFYTFPYIAHFGFVL
                     VMFAVESLFTFEVKFAYSHAGEAATENNNPPATDDRPDWKKNAEMREEARKERERLRE
                     PPEDPDSAEERPLYVRVLGSLEVRLNGVKIAAEELGGKKKLLTLFKILLVRYGKGIHR
                     EELLELLWPDLTEANALNNLHALIFRLRKVLTVSEAVAFISDTVSLDPSLVSTDAARL
                     DERIAESLALLSSHPERAITLMEKATDLYRGDYFEFDPNFEGTQMIRDQIATRFKKSL
                     LKVCSEITKENQSGSLIHFAEKCISLDNLDEECWRQLLRGLALAGKKNEARRKYDQLK
                     TLLQKELSVLPDAQTDDLMRRIGS"
BASE COUNT          514 a          539 c          557 g          586 t
ORIGIN      
        1 gtttcccact cttgcgtggg gagcgttttt caatctggag agaacagatc gtcgcgggga
       61 ttttctggaa tgcttgagtg gagatgagaa ttttctggaa acgccattct atgggagtcc
      121 acacaaccct gcgcgcggaa gaaaattgcg taagtaatgg tttgtgttcc ccgtttgtgt
      181 ttcgcaaata ctggatcact gctgcgttag cgtcgcagag taatcgattt ttttcctgaa
      241 ggccgaatga tttctccgcg ccccgagtct cgagcgagat ccaggagaat gagtccttct
      301 gcatcgtctg cgagcacgac ttgatcgtgc agctcagcaa tgaattgatt cggtccgggc
      361 atcgcatttc cgccgagagg tttttttccc gcagggccaa tcagatcctg gcctgatttt
      421 tcccaggatt cgtccgttgt gcgaaggccg tctgttgcca tcccgcgcca gttcccgaat
      481 ttttctatga gtactcgcac gcgacatcct gtgcccaggc atacagccac tcttcccgct
      541 gtggcgagac ctgcacctgc cgtgtctaca tctttgcttt tgacgcgaac ccgaaagaat
      601 ttttgaccgg tcagttctat agttccattc agtccggcgt cttccgacgt catggtccag
      661 gaccccgatg ggtttcgaga acagcctgcg aagaaagcaa gtaaaaggag gattacacgc
      721 atgcgcaagt cgcgcactcc agcatcctgg atgcaagtag attcaaaaga tcatgagccg
      781 attctgcgca tgagatcatc cgtttgggca tcaggcagta cactcaattc cttttgtagc
      841 agggttttga gttgatcata ctttctcctg gcttcattct tctttcctgc cagggccagt
      901 ccgcgcaaca gctgtctcca gcattcttca tccagattgt ccagagagat gcatttctct
      961 gcgaagtgaa tcaacgaacc ggactggttt tctttcgtga tctcggagca taccttcagc
     1021 aatgacttct tgaacctggt cgcaatctga tcacggatca tttgtgtccc ttcgaagttc
     1081 gggtcgaatt cgaagtaatc cccgcgatac aggtccgttg ccttttccat cagtgtgatg
     1141 gcgcgttctg gatgggagga aagcaaagcg agagactccg cgattcgctc gtccagtctg
     1201 gcggcgtctg tggatacgag agaaggatca agggaaacag tatcgctgat gaatgcgact
     1261 gcttccgata cggtgagaac tttccgtaag cgaaaaatca aggcatggag attgttgaga
     1321 gcattggctt ccgttagatc cggccacaat agttccagca gttcttcgcg gtggattcct
     1381 tttccatatc gcacgagcag gatcttaaaa agggttagaa gctttttctt tccaccgagc
     1441 tcttctgccg ctattttcac accattcaat ctcacttcga gagagccaag cacccggaca
     1501 taaagaggac gctcttccgc tgaatcagga tcttctggag gttcacgcaa ccgttctctt
     1561 tccttgcgtg cctcttcgcg catttcagca ttttttttcc agtccgggcg atcgtccgtg
     1621 gctggtggat tgttattttc agttgctgct tcgcctgcgt gtgagtatgc gaacttcact
     1681 tcgaatgtaa aaagactctc tactgcaaac attaccagaa caaatccgaa gtgcgcaatg
     1741 taggggaagg tatagaattc cagggaaacc gccatgtcat tggcgcccat caggaaaaac
     1801 aagacgagcg atacgacgag tatgcgcgat tctatttgtc tgaacagtcc aagttggaga
     1861 tatcgtacga tgatgccggc cattccaatt agattgagcg caatgaacat gaacacccat
     1921 actggttgtt ttgtttcgta gaatgtgaca cccagagctg gaaatgtttt cactgctccc
     1981 agctcgctgt tgtacgcgtg agggtaaaac aggatgatca gagaaagcat tgcatctgcg
     2041 atcgtgaaga cccagacaaa ccagtccggc agaatgggtc tgaatctttg cgcgaagaac
     2101 aggaagaaga cagcaatgaa cggcaggatt gcattttcta tgcgctcgaa tagaagcgcc
     2161 tgagattcat ttgtgttgat tgttttgaga aggcag
//
LOCUS       DAOQJL010000616         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38645_length_2193_cov_4.138447, whole genome shotgun sequence.
ACCESSION   DAOQJL010000616 DAOQJL010000000
VERSION     DAOQJL010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38645_length_2193_cov_4.138447"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PLG78_18775"
     CDS             <1..1676
                     /locus_tag="PLG78_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNE25225.1"
                     /translation="MAQGAKPGEGGQLPGHKVDKHIGRLRFSTPGVTLISPPPHHDIY
                     SIEDLKQLIFDLKNVNPKARISVKLVSEVGVGTVAAGVAKCHADHILISGDSGGTGAS
                     PISSIHHAGTPWELGLAETHQTLVANGLRDRVVLATDGKLLTGRDVVIGALLGAEEFG
                     FSTSALVSVGCIMMRKCHLNTCPVGVATQDEELRKKFHGKPENVINLMTFIAQEVREI
                     MAQLGFRKFSDMVGQVERLTFARPHKHWKARGLDFSKILSKVKPIFPTDLYCTKSQDH
                     GLDVQLDRELIERCKPALEKGEKVAFDITIRNTNRTVGAMLAGESAMRYGESGLPDGT
                     IHIRFFGVAGQSFGAFVNKGITLEIFGQVNDYVGKGLSGGRLIVRVPEGTSYDPAKNI
                     IAGNTCLYGATGGEAYINGLAGERFGVRNSGAHAVVEGLGDHGCEYMTGGRVVVLGKT
                     GRNFAAGMSGGIAYVFDMDGTFPRMVNPGMVDLEEPDARDEDEILQLVTRHKQYTGSL
                     VAEFILGDWDRLRRKFVKVIPGDYKIALKKIEEEHQLLTEPTETQSAGH"
     gene            1687..>2193
                     /gene="gltD"
                     /locus_tag="PLG78_18780"
     CDS             1687..>2193
                     /gene="gltD"
                     /locus_tag="PLG78_18780"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679307.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HNE25226.1"
                     /translation="MGKPTGFMEFARASLKVEAPADRIKHYKEFEKTPDAETAQSQGA
                     RCMDCGIPFCHGEYGCPTDNLIPEFNDLIYKGRWRDALENLHSTNNFPEFTGRLCPAP
                     CESACVLGIIEPAVTIKYNERTIIDRGFSEGWVEPQAPPKLLGTTVAVVGSGPSGLAC
                     AQQLARAGH"
BASE COUNT          541 a          521 c          626 g          505 t
ORIGIN      
        1 agatggcaca gggcgcgaaa ccaggagaag gcggacagct gccgggccac aaagtggaca
       61 aacacattgg tcggctgcgt ttcagtacgc caggtgtgac cttgatcagc ccaccaccgc
      121 accacgatat ttattcgatt gaagatttga aacagctgat cttcgatttg aaaaatgtga
      181 acccgaaagc gcgtatcagc gtgaagctag tgagcgaagt tggcgttggc actgtggctg
      241 ccggcgtggc aaaatgccat gcagatcata ttctcatcag cggcgactca ggcggaacgg
      301 gagcgagtcc aatttcatct attcatcacg caggtactcc ctgggaactg ggtctggcgg
      361 aaactcacca gacgttagtt gccaatggtc tgcgtgaccg cgtcgtgctt gcaacagatg
      421 ggaagcttct tactggccgc gatgttgtga tcggtgcact gcttggcgcg gaggaatttg
      481 gtttcagtac ttctgctctg gtaagcgtcg gctgcattat gatgcgcaag tgccacctca
      541 atacttgccc ggtgggcgtg gcaactcagg acgaagagct tcgaaagaag tttcatggta
      601 agccagagaa cgtaatcaac ctgatgactt tcattgcgca agaagtgcgc gaaatcatgg
      661 cacagcttgg tttccgcaaa ttcagcgata tggtaggcca ggtagagcgc ctaacgtttg
      721 cgcgtccgca caaacactgg aaagcgcgcg gtctggattt ctccaaaatc ctttctaagg
      781 tgaagcctat tttcccaacg gatctttact gcactaaatc tcaggatcat ggcctggatg
      841 tgcagctcga ccgtgagctc atcgaacgct gcaagcccgc tcttgagaaa ggggaaaaag
      901 ttgcatttga tatcacgatc cgaaacacga atcgcaccgt gggtgccatg cttgccggtg
      961 aatctgccat gcgatatggc gaatccggtt tgccggacgg aaccattcac attcggttct
     1021 ttggcgtagc agggcagagc tttggcgcat ttgtgaacaa aggcatcact cttgagatct
     1081 ttggccaggt gaacgactat gtgggcaaag gtctgtctgg cggtcgtctc attgtgcgag
     1141 ttccagaagg aacaagttat gatcctgcca aaaacatcat cgctggaaac acctgtctgt
     1201 atggtgcgac cggtggagaa gcttatatca atggtctggc cggagaaaga ttcggagttc
     1261 gtaactccgg agcgcatgcg gttgtggaag gactgggaga tcatggttgc gagtacatga
     1321 ctggcgggcg cgtggttgtg ctcgggaaga ctggccgtaa cttcgcggcc ggtatgtcag
     1381 gcggcattgc gtacgttttt gatatggacg gaacattccc gcggatggtc aatccgggga
     1441 tggtggatct agaagaacca gatgcacgcg atgaagatga aattcttcaa ctcgttacgc
     1501 gccacaagca atacacaggt tcactcgtcg ccgagttcat tctgggcgac tgggaccgtc
     1561 ttcgtcgtaa atttgttaaa gtaattcctg gcgattacaa gattgctttg aagaaaattg
     1621 aagaggagca ccagctcctg actgagccga cagaaacgca gtcggccggg cactgagagg
     1681 aacgctatgg gtaagcctac tggattcatg gaattcgcgc gtgcttctct taaagtagaa
     1741 gcgcctgcgg atcgcatcaa gcattacaaa gagtttgaga agactccgga cgcagaaaca
     1801 gcgcaatctc agggcgcgcg ctgcatggat tgtggcattc cgttttgcca cggagaatac
     1861 ggctgtccaa cggacaatct catacctgaa ttcaatgacc tgatctacaa aggtagatgg
     1921 agagatgctt tagaaaattt acattctacg aacaactttc ctgagttcac cggaagattg
     1981 tgtccggctc cgtgcgaaag cgcctgcgtg cttggaatca ttgaacctgc tgtcacaatc
     2041 aagtacaatg agagaaccat cattgatcgt ggcttttcgg aaggatgggt agaaccgcag
     2101 gctccaccaa aactactcgg aaccacggtg gctgttgttg gatcgggccc gtccggtctt
     2161 gcatgtgccc agcagcttgc gcgggcagga cac
//
LOCUS       DAOQJL010000617         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38782_length_2189_cov_2.673852, whole genome shotgun sequence.
ACCESSION   DAOQJL010000617 DAOQJL010000000
VERSION     DAOQJL010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38782_length_2189_cov_2.673852"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..455
                     /locus_tag="PLG78_18785"
     CDS             213..455
                     /locus_tag="PLG78_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25227.1"
                     /translation="MSRFSFSFAALALILGFFMGSVLGSLLKQVFGLSFLDDPLFSDS
                     LVVAKDFYAIKRLEIRLTAAGLFGLIAAGWFLYKKL"
     gene            499..1389
                     /gene="rpsB"
                     /locus_tag="PLG78_18790"
     CDS             499..1389
                     /gene="rpsB"
                     /locus_tag="PLG78_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNE25228.1"
                     /translation="MSTISMKNLLEAGVHFGHQTRKWDPRMAPYIFTARNGIHIIDLQ
                     KTVQLAKQAYEALRAFTAQGQRVLFVGTKKQARAAIEKEGARSNMYYVNTRWPGGLLT
                     NWPTVKKSIVRMKKLEAMEAAGSFDQEARTKKEALLLQRELDKLRKHLSGIKELQSPP
                     EILFVIDPSKEDIAVKEARAMGIKIFAVVDSNCNPELIDYPIPGNDDAIRAISLYLQT
                     MADAVLEGTQGVEQGSRFTDEDATMDPDAIALDSLQYRGEYDESGEFIPDDANSPKTN
                     PDEEAAVKEYVAPPAAPKES"
     gene            1404..1997
                     /gene="tsf"
                     /locus_tag="PLG78_18795"
     CDS             1404..1997
                     /gene="tsf"
                     /locus_tag="PLG78_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNE25229.1"
                     /translation="MAEVKSDDIKKLREMTGAGMMDCKSALAENAGDIEKASEALRKK
                     GLAKAAKRMDRDTGSGRVFSYIHGEGKIGVLLQLNCETDFVAKNEEFEQLGKDLCMQV
                     AAASPLAIKAEDLDPKIIEKETEVYREQLIQEKKKPEQIEKILPGKIKKFQQDVCLLD
                     QFFIKDNKQTVAELIKSFIGKFGENITISRFTRYEAG"
BASE COUNT          613 a          512 c          574 g          490 t
ORIGIN      
        1 cagaaacgag gtgtctccgg aaagagcggc ggcgcttttc ggagtggatg aaatcttaga
       61 ccccagagat acgggcccgg ttctcgaacg gttgcttgat ttgactgccg gtaaaagggt
      121 ccttcgcccg gagcgcaagc acgggatttt tccagtatga attcgtttga cgtagaccct
      181 gttgtattcg gttatggtcg aggcgtacct ggatgagtcg tttttcgttc agtttcgccg
      241 ctcttgcgct gattcttggc tttttcatgg ggtcggtgct gggctctctc ttgaagcagg
      301 tcttcggact gagctttctg gatgatccgt tgttctccga ttcactcgta gtggcaaagg
      361 acttctatgc aatcaagaga ttggagatca ggctgactgc agccggtctt tttggactaa
      421 tagctgccgg atggttcctc tataaaaagt tgtgagccag ggcaaggcgc aatgcgccag
      481 ggaatgtgag gagatagaat gtctacgatt tcaatgaaga acctgctgga agcaggcgtc
      541 cactttggcc accagacacg gaagtgggac ccaaggatgg caccgtacat ctttaccgca
      601 agaaacggaa ttcatatcat cgatttgcaa aaaacagttc agctggcaaa gcaggcatac
      661 gaagcacttc gtgctttcac agcccagggc cagagagttc tcttcgtggg aacaaagaag
      721 caagcgcgcg ctgccattga aaaagaaggc gcacgttcta acatgtatta tgtgaacact
      781 cgctggccag gcggactcct caccaactgg ccaactgtaa agaaatccat tgttcgcatg
      841 aagaaactcg aagcaatgga agcggccgga tcttttgatc aagaagcgcg cacaaagaaa
      901 gaagcgctct tgcttcagag agaactggac aagctgcgca agcatctttc cggtatcaaa
      961 gagctccagt ctccaccgga aatcctcttc gtaattgatc caagcaaaga agacatcgca
     1021 gtcaaagaag cgcgcgcgat gggcatcaag atctttgctg ttgtggattc caactgcaac
     1081 ccggaactca ttgactatcc aattccaggc aacgatgacg cgatccgtgc catctctctc
     1141 tatctgcaaa ctatggcaga tgcggttcta gaaggaaccc agggagtaga gcagggctct
     1201 cgctttacag atgaagacgc aactatggat ccagatgcga tcgctctgga ttcactgcaa
     1261 tatcgcggtg agtacgacga gtccggagaa ttcatcccgg acgatgcgaa ttctccgaag
     1321 accaatccag atgaagaagc agctgtgaaa gaatatgtag ctccacctgc tgctccaaaa
     1381 gaatcctgag gaattagacg atcatggcag aagttaaatc cgacgacatc aagaaactgc
     1441 gcgaaatgac cggcgcaggc atgatggact gcaagtctgc tctggctgaa aacgccggcg
     1501 atatcgaaaa agcaagcgaa gctcttcgca agaaaggtct cgcgaaagca gccaaaagaa
     1561 tggatcgcga cactggatcc ggccgtgtgt tttcctacat tcacggagaa ggcaagattg
     1621 gcgtattgct gcagctgaat tgcgaaactg atttcgttgc aaagaatgaa gaattcgagc
     1681 aactcgggaa agatctttgc atgcaggtcg cagcggcaag ccctcttgcg atcaaagcag
     1741 aagacctcga tcctaaaatc atcgagaaag aaacagaagt atatcgcgaa cagctgattc
     1801 aggaaaagaa gaagccagag caaatcgaaa agattctgcc aggcaagatc aagaagtttc
     1861 agcaggatgt ctgccttctc gatcaattct tcatcaaaga caacaagcag actgttgcag
     1921 aattgattaa gtcattcatt gggaaattcg gagagaacat tacaatctcc cggtttaccc
     1981 gttacgaagc gggctgataa aggaggctca cgtgaatccg aattttccat tccgtcgggt
     2041 tctactgaag ctttcgggtg aagcctttac aacggaggga aaactcggta ttgagttttc
     2101 ccgcgttcgt cacatcgcag aacaaatcaa agcagtgcac cgcatgggtg cagaaatcgc
     2161 agtggtcgtt ggcgccggaa atatctttc
//
LOCUS       DAOQJL010000618         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38952_length_2184_cov_4.004227, whole genome shotgun sequence.
ACCESSION   DAOQJL010000618 DAOQJL010000000
VERSION     DAOQJL010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38952_length_2184_cov_4.004227"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="lpxK"
                     /locus_tag="PLG78_18800"
     CDS             complement(<1..714)
                     /gene="lpxK"
                     /locus_tag="PLG78_18800"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNE25230.1"
                     /translation="MMCPRFLSQIYLYFHHRKFRARAREARSFAGRRIISIGNLSMGG
                     TGKTPLVEMLAREALKRGHRPMVVLRGYKGSTSGLVTDGQNVKMAFAEAGDEAMLFTQ
                     TPGLRVAVGRDRASVIDQFGGDSTLIFLDDAFQNPSVKRQVDLVLIDASIPENKFRVF
                     PCGKFREGLEALSRASAVVLTRADSKTAPEWKSRIQASFPRLRVFESTHEFAGLRPVL
                     DRGPSASRPVTASAFCGIGN"
     gene            complement(725..>2184)
                     /locus_tag="PLG78_18805"
     CDS             complement(725..>2184)
                     /locus_tag="PLG78_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE25231.1"
                     /translation="DLRMELYNKLNVLGLEHFTENQTGFIMSRVINDAEAVGRSLSAE
                     FQEGLVDLLYIITHFALLAVISWQMLLMALLIVPILSSPVAKFAKKIRGAAMGQQERL
                     ADLGAHIHEILSGIRVIRAFSMQKFELGRFQQVNQKLYQNTFRGHYYHQVGPALTEFV
                     ATIAVVGFLSWGAYRIAASTMNKGQFFTFFFTLIFLMRPIKQVSVLANLTSVARAAGQ
                     RIFEVLDKPVTVTDVKDPKRLRALSDEIVYDNVSFRYPGHDRTVLDGVSFRVKQGQTI
                     SFVGASGAGKSTLMDLLPRFFDTTSGSIRIDGIDIRQYSLRDLRTAIGIVTQNIFLFH
                     TTVRENISYGRLDIPMERIVEVARAANAHDFISELPQGYETNIGESGVMLSGGQRQRI
                     AIARAILLDPPVLVFDEATSALDNESEKLVHEAMERLLLGRTVFIIAHRLSSVYRSDC
                     IYVMENGKIVEQGKHEELLEAGAVYKNLYEMQFQG"
BASE COUNT          545 a          591 c          540 g          508 t
ORIGIN      
        1 gtttccgatg ccgcagaagg cggacgccgt gacaggacga cttgcggatg ggccgcggtc
       61 caggactgga cgtagacctg caaactcatg ggtggattcg aaaactctca agcgaggaaa
      121 gctggcctgg attctggact tccactccgg tgcggtcttt gagtccgcgc gtgtcaaaac
      181 cactgcgctc gcgcgcgaaa gcgcttcgag cccttctcta aattttccgc agggaaatac
      241 gcgaaacttg ttctccggaa tgcttgcatc gatcagcacg agatccacct ggcgctttac
      301 agatgggttt tgaaacgcat cgtccagaaa gatcaaagta gaatctccgc caaattgatc
      361 gattacagac gccctgtctc tgccgacggc cactcgaaga cctggagtct gagtgaagag
      421 catggcctca tcccccgcct ctgcgaaggc catctttacg ttttgtccgt ccgtgaccag
      481 accactggta gatcctttgt agcctcgtag caccaccatc ggcctgtgcc cccgcttcag
      541 tgcttctctg gcgagcattt ctacgagcgg agtttttcca gttccgccca tggaaaggtt
      601 tccaatagaa atgattctgc gccccgcgaa tgaacgcgcc tctctggcgc gggcgcgaaa
      661 ctttctatga tgaaagtaga gataaatctg agaaagaaaa cgcgggcaca tcatgggccc
      721 tgcatcaacc ctgaaattgc atttcgtaca gattcttata caccgctccc gcctcaagga
      781 gctcttcgtg tttcccctgt tccacgatct ttccattttc catgacgtaa atgcagtctg
      841 atcgatacac actggataga cgatgcgcta tgataaagac tgtcctgccg agcaagagcc
      901 tttccatggc ctcgtgcacg agcttctcgc tttcattgtc gagcgcggat gtggcttcat
      961 caaacaccag aacaggagga tcgagcaaga tggcccgcgc aattgcgatg cgctgtcttt
     1021 gccctccaga aagcatcact cctgattctc caatgtttgt ttcgtaaccc tggggaagtt
     1081 cgctgatgaa atcatgcgca tttgccgcgc gtgccacctc cacgattctc tccatgggga
     1141 tatccagtct accgtaggaa atgttttctc gcacagtagt gtgaaaaagg aaaatatttt
     1201 gcgtcacaat tccaatggcc gtgcgaagat ctcgcagact gtactgccga atgtcaatgc
     1261 catcaatgcg aatgcttccg gatgtagtgt caaagaatcg cggaagcaga tccatcaggg
     1321 tggacttgcc tgcgccagac gctcctacaa atgaaatagt ctggccctgc tttacccgaa
     1381 aactcacacc gtctaagacc gtgcggtcgt gacccggata ccgaaaagag acattgtcat
     1441 acacgatctc atctgaaaga gcacgaagtc gtttcggatc ctttacatcc gtcacggtga
     1501 ctggcttatc cagaacttcg aaaattctct ggcccgccgc gcgcgcaacg cttgtcagat
     1561 tggcgagaac ggatacctgt ttgatgggac gcatcagaaa gatcaaagtg aagaagaagg
     1621 taaagaattg gcctttgttc atggtgcttg cggcgatgcg atacgcaccc cacgacaaaa
     1681 agccaaccac agcgatggtt gctacaaact cagtaagagc ggggccaact tgatgatagt
     1741 aatgtccgcg aaatgtattc tgatagagtt tctgattcac ctgctggaat cttccgagtt
     1801 cgaacttctg catggaaaat gcacgaatga cccgaatgcc gctcagtatt tcgtgaatgt
     1861 gtgcacccag atccgcaagc ctctcctgct ggcccatggc cgcaccgcga atcttctttg
     1921 caaatttcgc aacaggcgaa gatagaatgg gcacaatcag aagagccatc agcagcatct
     1981 gccaggaaat cactgcaagc aaggcgaaat gagtgatgat gtagagcaaa tcaacgaggc
     2041 cttcttgaaa ttctgcggaa agacttctgc cgactgcctc cgcgtcattg atgacacgac
     2101 tcatgatgaa tccagtctgg ttctccgtaa aatgctcgag ccccagaacg ttgagcttgt
     2161 tgtaaagctc catgcgcaaa tcgc
//
LOCUS       DAOQJL010000619         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38991_length_2183_cov_4.056861, whole genome shotgun sequence.
ACCESSION   DAOQJL010000619 DAOQJL010000000
VERSION     DAOQJL010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38991_length_2183_cov_4.056861"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLG78_18810"
     CDS             <1..519
                     /locus_tag="PLG78_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNE25232.1"
                     /translation="GTTRLEYIVPTRGLIGFRSFLITESRGSAAQNSRFLHYAPYAGP
                     IPGRKNGALVSMEAGTTVAFALWNIQERGSLFVDPGIPVYGGMIIGECAREQDLDVNP
                     AKEKKLTNMRTTSADEAVRLVPPRKLSLEQSLEFIDDDELLEVTPKSLRLRKKLLDPN
                     ERKRHARLAGTT"
     gene            494..>2183
                     /locus_tag="PLG78_18815"
     CDS             494..>2183
                     /locus_tag="PLG78_18815"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/class IV aminotransferase"
                     /protein_id="HNE25233.1"
                     /translation="MRGWPELHNAVIFEDAGNLPRLFEYPEYVIETALEKDIASALRK
                     ADEASREGLYVAGFLSYDVGQGARGSIFPKPIPRLWLGCYSSFSRASFLEDSTSSHYS
                     LKAIPPSMDERDSFMRSVEQIRESIRSGIVYQVNHTRRKLYDMKGSALALYARLRSLQ
                     RARYAAFVSIPDFTVLSLSPELFFSLEDDWIESHPMKGTFSNFISRNEIDADEKSLSE
                     NHMILDLVRNDIGRIAEPGSFSLDPARIVQLSTLQQMISVVRARLKKHTGLDQIFAAL
                     FPPGSVTGAPKRTAMQSIDQLEPARGVYTGAIGVVEPFRRSAVFNVAIRTLQIQNGHA
                     VYGTGCGIVWDSVPSSEWAESELKTAWLKPALDDFHIIETMRMDQGRVFLLRAHLRRM
                     QKSAEFFKMDFSVRDALQKISKEKQADRPLRIRLALYATGIEIKIAPAPPFRICGTSR
                     VIISQDAIHSGDPFRIHKTSERSLYDELFRKANDLGFDDAIFLNETGEVAETAICNLM
                     VKKGMVWVTPEPSAGALPGIMMRECMRRFPGRIVFGTILAEDLKGRVLVCNSVRG"
BASE COUNT          525 a          550 c          562 g          546 t
ORIGIN      
        1 ggcacaactc gtcttgaata cattgtgcct actcgcggcc tgattggatt tcgcagcttc
       61 ttgatcacag aaagtcgcgg ttctgcggcg cagaactctc gctttttgca ctatgctccg
      121 tatgccggtc ctatcccggg aagaaaaaac ggcgctctcg tttctatgga agctggcaca
      181 actgttgctt ttgctctctg gaacattcag gaacgcggca gtctttttgt agatccgggc
      241 atcccggtct atggcggcat gatcattggc gagtgtgcac gtgagcaaga tctggatgta
      301 aaccctgcca aagaaaagaa actgaccaac atgcgcacga cctctgcgga tgaagcggtt
      361 cgcctggttc cacctcgcaa gctcagcctg gaacaatctc ttgaattcat cgacgatgat
      421 gaactacttg aggtgactcc aaagagcctt cgccttcgca aaaagctgct cgatccaaat
      481 gaacgtaagc gtcatgcgag gctggccgga actacataac gcagtcattt tcgaagacgc
      541 gggcaatttg ccgcgtcttt tcgaatatcc agagtatgtc attgagactg ctctggagaa
      601 agacatagcg tccgcacttc gaaaagcaga cgaagcatcc cgcgaaggcc tgtacgtggc
      661 cgggttcctt tcttacgacg tcgggcaggg cgcccgtgga tccatattcc caaaacctat
      721 ccctcgtctg tggctgggtt gttattcttc tttttcgcgt gcgagttttc tagaagattc
      781 tacctcatcg cattacagcc tgaaggcgat tccaccgtcg atggacgaac gagactcgtt
      841 tatgcgatct gtggagcaga tccgagaatc tattcgttct ggaattgtat atcaggtcaa
      901 tcatactcgt cgcaaactct atgatatgaa ggggagtgct ctggctctct atgccagact
      961 gcgatctctg cagcgggccc gttatgcggc atttgtttct atccctgact tcactgtctt
     1021 atcactgtca ccggagctat tcttttccct cgaagacgat tggatcgaga gtcatcccat
     1081 gaaaggtacg ttttcgaatt tcatctccag gaatgagata gatgcggatg aaaagagtct
     1141 ctctgaaaat catatgatac tggacctggt cagaaatgac attggccgca tcgcagaacc
     1201 gggcagtttt tccctggacc ccgcgcgcat tgtgcaactc agtactcttc agcagatgat
     1261 atcagtcgtg cgcgctcgtt taaaaaagca cacgggcctc gatcagattt ttgcggcgct
     1321 ctttcctccc ggatctgtga caggtgcacc caaacgaaca gctatgcagt cgatcgatca
     1381 actcgagccg gcgaggggag tttatacagg cgccatcgga gtcgtagagc cgtttcgtcg
     1441 atctgcagta ttcaacgtag ccattcgcac gctccaaatc cagaatggcc atgcggtcta
     1501 cggcaccggg tgcggaatcg tgtgggattc tgttccgtct tctgaatggg cggaatctga
     1561 gttgaaaacg gcctggctca aaccagcgtt agacgacttt cacatcattg aaactatgag
     1621 aatggaccag gggcgcgttt ttcttttgcg cgcacatctt cgaagaatgc agaaatcggc
     1681 ggagtttttc aagatggact tttctgtacg ggacgctttg cagaaaatct ccaaagagaa
     1741 gcaagcagac cgacctctgc gcattcgcct ggccctgtat gcaacaggta tcgaaatcaa
     1801 aattgcgcct gcgccgccgt ttcgaatttg cggaacatca agagtgatca tctcgcagga
     1861 tgcaattcat tcgggtgatc cgtttcgtat ccacaaaaca agcgaaagga gcttgtacga
     1921 tgaattgttt cgcaaagcaa atgatctcgg gttcgatgac gcaatcttcc tgaacgaaac
     1981 aggagaggtc gcagagactg ccatttgcaa cctgatggtg aaaaaaggaa tggtctgggt
     2041 cacgccggag ccttctgcgg gagcgctccc aggaatcatg atgcgcgaat gcatgcgacg
     2101 ctttccgggc agaattgtat ttggaactat acttgccgaa gatctaaaag gtcgggtcct
     2161 cgtatgcaat tctgtgcgag gca
//
LOCUS       DAOQJL010000620         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_38996_length_2183_cov_3.699248, whole genome shotgun sequence.
ACCESSION   DAOQJL010000620 DAOQJL010000000
VERSION     DAOQJL010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38996_length_2183_cov_3.699248"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..361)
                     /locus_tag="PLG78_18820"
     CDS             complement(20..361)
                     /locus_tag="PLG78_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25234.1"
                     /translation="MKKTLLISVTLTLSALLLVNANCKKKGPDLSKYDEICATVTKCD
                     QNPQSKMLLMQPEACQKMFAGLDKSPQTAAFTPQVIDCIQKAPCAKLDTMKCFAASAQ
                     GIMPMMPAPNK"
     gene            complement(438..>2183)
                     /locus_tag="PLG78_18825"
     CDS             complement(438..>2183)
                     /locus_tag="PLG78_18825"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE25235.1"
                     /translation="EIQTFRTIKGVHVDLPPALLREDNTLVFHIRGTAAVTSLYLNDY
                     SGFYMRNGYEIDTAENLRAKNNELFSFMLYSVYFFFGLYHIILYLSNRQQRYNLSFGL
                     LSVMLAFYSILISNSVFSIFPTMDTAILKKAELVSLFCLMPGISYFIVDYFEPGRFKW
                     ALRPALVWNISLALVAGFLPFQYARVALPIWQLTALVQLPFLLFLMIRYVIARDPDAY
                     VMGATFLLALLSAVFDIVASFLVVSDVRLFQATFFLFILSIVGTIALRLRRLEKSERA
                     LNDELRELSLAFYRFVPTQVLEQMEKSSAADLKVGDNALRLMNVLFSDIRGFTTLSET
                     MNPDDNFRFLNSYLARMEPLIAAESGYVDKFLGDGIMALFSQAAGEVQSKTPSERGLD
                     AAIAMRRTMPDYNNRRKNSGYPPIDIGIGLHTGPLIIGAVGSSNRIDTTVIGNTVNVA
                     SRIEGMTSYYKAGIIISEALRTDLSSPDKYRIREIGSILVKGKTHPISLYEVFDADPE
                     EIAEFKSRTSAFISVGIAHFREKEFAEARKLFREVLLEYPADSVAKVYYKLCTKFLSG
                     PVPENWTGAIEVLRK"
BASE COUNT          576 a          495 c          562 g          550 t
ORIGIN      
        1 ttcttatgag tgaagcgtat tatttgtttg gagctggcat cattggcatg ataccctgag
       61 cagatgctgc gaagcatttc attgtatcca gtttagcaca tggcgctttt tggatgcaat
      121 cgatcacttg aggtgtaaaa gcagccgtct gtgggctctt gtccaggcct gcgaacatct
      181 tctggcaggc ttcaggctgc atcagaagca tcttcgattg cggattctgg tcgcatttgg
      241 ttacagtggc gcaaatctca tcgtatttgg agagatcggg gcctttcttc ttgcagtttg
      301 catttaccaa aagcaaggcg gatagcgtca gagtcacact gatcagaagt gtttttttca
      361 tgcggcaatt ttgcaacaaa atgccacatt gtcaacccat tcgagcgtgc tgttttcgcc
      421 acccgaatgc cgcaacatca ctttcgcaga acctcgatgg cgcccgtcca gttttctggc
      481 acaggtcccg acaggaattt ggtgcagagt ttgtagtaca ctttcgccac agagtcagcc
      541 ggatactcga ggagcacctc gcgaaagagc tttcttgctt ccgcaaattc tttttctctg
      601 aaatgagcga ttccaacaga aatgaatgcg ctggttctgg acttgaattc agcaatttct
      661 tccgggtccg cgtcaaagac ttcatataga gagatcggat gcgttttgcc tttcaccaga
      721 atggagccga tttctctgat tcgatatttg tcaggcgagc ttagatccgt gcgaagtgcc
      781 tctgaaatga tgatgcccgc cttgtagtaa gacgtcatcc cttcaatacg agaggcaacg
      841 ttgaccgtgt ttccgattac agtagtatcg attcgattgg agctgcctac cgctccaatg
      901 atcagtggac cagtgtgcag accgattcca atgtcaatag gcgggtagcc cgagtttttt
      961 ctgcgattgt tgtaatcagg catcgtgcgg cgcatggcaa tggccgcgtc gaggccgcgc
     1021 tcagatggag tcttggattg aacttctcct gcagcctgtg agaacaaagc catgatgcca
     1081 tcgcccagga atttgtctac atagccactt tctgccgcaa tcagaggttc cattcgcgcc
     1141 aggtacgaat tcaagaagcg gaagttgtca tccggattca tggtttcgga cagagtcgtg
     1201 aatccgcgaa tgtcagagaa cagcacgttc atgagccgaa gggcgttgtc cccgaccttg
     1261 aggtctgcgg cagatgattt ctccatttgt tcgagcacct gcgttggcac aaatctataa
     1321 aacgcaaggg aaagctcgcg aagctcatca ttgagtgctc tttcggattt ctctagacgc
     1381 ctgagcctga gtgcgatggt tccaacaatc gataggatga acagaaagaa cgtggcttga
     1441 aacagacgaa cgtcagagac cacgagaaaa gacgcaacaa tgtcaaagac tgcagacaag
     1501 agggcgagaa gaaaagtggc ccccattacg tatgcgtctg gatcacgtgc gatcacgtaa
     1561 cggatcatca aaaacaaaag gaacggaagc tgtacgagcg ctgtcaattg ccagatcggg
     1621 agtgccactc ttgcgtattg aaatggaaga aacccggcca caagtgccag agagatattc
     1681 cacactagag ctggccggag tgcccatttg aatcgcccag gttcaaaata gtctacaatg
     1741 aaatagctga tcccaggcat cagacagaac agagaaacaa gttcggcctt ctttagaatc
     1801 gccgtgtcca tggttgggaa aatggaaaag actgaatttg agatcaagat actgtagaat
     1861 gcgagcatca cggagagcag accgaaggaa aggttatatc tttgctggcg attgctcaag
     1921 tacagaatga tgtgatatag accaaagaaa aaataaacag aatagagcat gaaggagaaa
     1981 agttcgttgt tcttcgcacg caggttttcc gctgtgtcga tttcgtaacc gtttcgcatg
     2041 tagaatcctg aataatcgtt caggtaaaga gaagtgacgg ctgcagttcc cctgatgtga
     2101 aagacaagtg tgttgtcttc gcgtagcaat gcaggcggaa gatctacatg tactcctttg
     2161 attgtgcgaa atgtctggat ttc
//
LOCUS       DAOQJL010000621         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_39440_length_2169_cov_3.587039, whole genome shotgun sequence.
ACCESSION   DAOQJL010000621 DAOQJL010000000
VERSION     DAOQJL010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39440_length_2169_cov_3.587039"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLG78_18830"
     CDS             complement(<1..842)
                     /locus_tag="PLG78_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE25236.1"
                     /translation="MNPARVQQILGSFDKIRILVLGDFMIDEYRYGRAERLSPEAPVP
                     VILETGVRQLPGGAGNVVKNLESLKIKNTALGVCGQDQEAEVMRGLLSGCEELSLIAD
                     RSRPTTKKMRIVAGTQQICRLDQEDASSVPREVEDEIVSRADRMIPSCDAVIISDYDK
                     GVVTERVIESCIRSSHEKVFISVDPQVSRFGFYHGVGVLTPNHHEAGRFIGMRLETDA
                     EVEKGGRLIMQKLSPRMLLITRGEKGMSLFEGDSVRHFPTRAREVFDVTGAGDTVITV
                     FTAV"
     gene            complement(842..1678)
                     /locus_tag="PLG78_18835"
     CDS             complement(842..1678)
                     /locus_tag="PLG78_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25237.1"
                     /translation="MKRVLFAILLFSSAALLAVEASYGIRKDHLQKTLGEGVYVDWTA
                     GYISVSADHKIEDVRAGGGLTRVRDDATQKAREKALSRLMQIVSTLPIHGHDTIESKL
                     ESDESLQNRMGMLASLPFEKARRVGDGSVSVELILPFYGRRGLLSRVYSGEESEMAEL
                     VHENPEDPITGIVVDATEASSDPVLEPRILTDQGRLIYGPGIARRGCYISRGMAAYYT
                     SREAARKDKRVGENPYLVYARGRKTPGDLFISSQDSVRILGSKSGRDALRKCAVTIVV
                     SK"
     gene            complement(1675..>2169)
                     /locus_tag="PLG78_18840"
     CDS             complement(1675..>2169)
                     /locus_tag="PLG78_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNE25238.1"
                     /translation="SGISYISQVSLGPPAALRAFHENPDSVLLGLSSFWQGIDVPGDA
                     LRLVVIVRLPFQVPDDPVLSARTEKLQNAGRSPFSEIQLPHAVLTVKQGFGRLMRRES
                     DRGIVSILDARLRTKSYGKDVLAALPRARLVSSFEDLQKSYQELFSDIQNVAEIPGRP
                     ILNR"
BASE COUNT          528 a          599 c          490 g          552 t
ORIGIN      
        1 atcactgccg taaaaacagt aattacagtg tccccggccc ctgttacatc gaacacttcg
       61 cgagctctgg ttgggaaatg acgcacggaa tccccctcaa agaggctcat tcctttttcg
      121 ccgcgagtga tgagtagcat tcgtggggaa agtttttgca tgatgagccg cccacctttt
      181 tccacttccg cgtccgtttc cagacgcatt ccaatgaacc tgcctgcctc atgatgattc
      241 ggagtgagta ctcctactcc atgataaaaa ccaaatcgac tcacttgagg atctacactg
      301 ataaaaactt tttcgtgaga cgaacggatg caggattcga tcactctctc cgtgaccact
      361 cccttatcat aatctgaaat gatgactgca tcgcaggaag gaatcatcct atccgcacgc
      421 gaaacgattt catcttccac ttcgcgcggc acactggatg catcttcttg atcgagtctg
      481 caaatttgtt gagttccagc aacaatcctc attttctttg tagtgggcct gcttctgtct
      541 gcgatcaaac tgagctcttc acatcctgat agcagacctc tcatcacttc cgcttcctgg
      601 tcctgtccac agacgcccag agctgtgttc ttgattttta aagattctaa gttctttaca
      661 acatttccag cgccaccagg aagctgacgc actcctgttt ccagaatcac agggaccggc
      721 gcttccgggg agagtcgttc cgctcttcca tagcgatact cgtctatcat gaaatcaccg
      781 agaacgagta tgcgaatctt atcaaacgag ccaaggatct gctgtacgcg agctgggttc
      841 attatttact tactacgatt gtgaccgcgc actttctcaa agcgtcccgg ccagattttg
      901 agccaaggat gcgcacggag tcctgagagg aaatgaataa atcgcccgga gtctttcgac
      961 cgcgagcata taccagatac ggattctcgc caactctctt atcttttcgt gccgcttctc
     1021 tcgatgtgta atacgctgcc atgcctctgg atatgtagca accacgacgg gcaatgcctg
     1081 gaccgtaaat caatcgtccc tggtctgtaa gaatacgtgg ttcgagtact ggatccgaag
     1141 atgcttctgt cgcatccacc acgattccgg tgatcggatc ctccgggttt tcatgaacga
     1201 gctctgccat ttcagattct tcgcctgagt acacgcgaga aagaagtcca cgacgaccgt
     1261 aaaatggtag gatcaattct acactcacgc ttccgtcgcc gactcgcctg gccttttcaa
     1321 aaggcagtga ggcgagcatt cccattctat tctgcaagga ttcatctgat tccagcttgc
     1381 tttcgattgt atcatgacca tggattggaa gagtggaaac aatctgcatc aggcgagaaa
     1441 gggctttctc tcttgctttc tgggttgcgt catcgcgcac ccgcgtgaga ccaccacccg
     1501 cacgcacgtc ttcgattttg tgatctgcgg aaacagaaat gtatcccgca gtccagtcca
     1561 catagactcc ttcgccgaga gtcttttgca ggtggtcttt gcgaatcccg tagctagctt
     1621 ccacggcaag caaagccgcc gagctaaaga gaaggattgc gaacaaaacg cgtttcatct
     1681 atttaatatc ggcctgccag gaatttcagc cacgttctga atgtcagaga aaagctcctg
     1741 ataggatttc tggagatcct cgaaactgga gaccagtcgc gccctgggca gagccgccag
     1801 cacatctttt ccataggact tcgttctcaa tctagcatcc agaatcgata caatgcctcg
     1861 atcactttct cttctcatga gtcgcccaaa gccctgcttc acggtcaaaa ctgcatgcgg
     1921 tagctggatt tctgaaaaag gacttctgcc cgcattctgc aacttttcag ttctagcaga
     1981 cagcacggga tcgtctggca cctggaaggg aagccttaca atcacaacga gcctgagggc
     2041 atctcctgga acatcaattc cctgccagaa actggaaagg ccaagtaaga cagagtccgg
     2101 attttcatga aacgctctca aagcggcggg cggaccaagg ctcacctggc tgatgtagga
     2161 gattccact
//
LOCUS       DAOQJL010000622         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_39458_length_2168_cov_5.310932, whole genome shotgun sequence.
ACCESSION   DAOQJL010000622 DAOQJL010000000
VERSION     DAOQJL010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39458_length_2168_cov_5.310932"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1171
                     /gene="bioB"
                     /locus_tag="PLG78_18845"
     CDS             77..1171
                     /gene="bioB"
                     /locus_tag="PLG78_18845"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNE25239.1"
                     /translation="MDLRERLKRVANGESMPVEADLALALRGEIPLSELLGIAEEPRR
                     KYFGNKIQVHILDNVRNGYCAEDCGYCAQRKTATEEIPAYSLKKEQEILKEAEVAFRS
                     GAYRFCIVTSGTGPSEKQTVQYADLIRKIKEKYPMRLCLSAGIIKDPHFAQILAEAGL
                     DRYNHNLNTSESHTAEIVTTHTFQDRIQTIENVRDAGISVCSGLIAGMGESPEDLAQV
                     AVRLRSLNVPSIPVNFFLPVPGHNVAHASTLSPDYCLRILCVYRIANPSAEIRIAAGR
                     EIHLKDKQQEAFRVGNSLFVSGYLNVKGSDARETYNLIHNAGFEIDADTSELDPEFRS
                     LIMGEQNSPAGAQSGDLRMKTREDLRSFAE"
     gene            complement(1183..1854)
                     /locus_tag="PLG78_18850"
     CDS             complement(1183..1854)
                     /locus_tag="PLG78_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25240.1"
                     /translation="MLEEVSKLIDSRETAGSLEKAIELLEKLKAENPEKDIIRGKLSS
                     AFFYKGLFSPEGTREREDAFDNGVKYGQEAITLNPRAVYGNFWYASNLGYLGMCRGAL
                     ASLASIDPFKKSMEVVLKENENFYFGGPHRAIGRLYHQAPGWPISIGNKNKAFEHLEK
                     CVQLAPNFMNNRLYLAELLIDMGKKDKAVEHLNFCKDTALNPGHEKEDGVYKEQAEKL
                     LKRIS"
     gene            1943..>2168
                     /locus_tag="PLG78_18855"
     CDS             1943..>2168
                     /locus_tag="PLG78_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE25241.1"
                     /translation="MQPFIAPGFNLPLAWTADLLFPVLIKAVQNIDDVEIRPEDKEML
                     RTLRNERLIFFTNHPTTAEPPIAYYIGNLMG"
BASE COUNT          580 a          541 c          507 g          540 t
ORIGIN      
        1 gataaaacgc gcgagggggg cttgttgccg ctctcgaaaa cgcttgttcg cctggtcgtc
       61 agtttttttc tgaaacatgg atctgcgaga aagactgaag cgcgttgcaa acggggagag
      121 tatgcccgta gaagcggatc tagcacttgc tttacgcgga gaaattccac tctccgaatt
      181 gcttgggatc gcagaagagc cgcggcgcaa atactttgga aacaagatcc aggtgcacat
      241 tctggacaac gttcgaaacg gatattgcgc ggaagactgc ggatattgcg cgcagagaaa
      301 gactgcaaca gaagaaattc ctgcctattc tttgaagaaa gaacaagaaa tactgaagga
      361 agctgaggta gcgtttcgct ctggcgccta tcgcttctgc attgtcacgt ccggcactgg
      421 tccctcggaa aaacaaactg tgcagtacgc agatctgatt cgcaaaatca aagagaaata
      481 tccgatgcgt ctctgcttga gcgcaggcat catcaaagat cctcacttcg cacagatact
      541 tgcggaagcg ggtctggatc gatacaatca caatctaaac acttcagaat cgcatacagc
      601 ggaaatcgta accacgcaca catttcagga tcgcattcag acgatcgaga atgtaaggga
      661 tgctggaatt tctgtgtgca gtggactcat tgcaggaatg ggcgaatccc cggaagatct
      721 cgcgcaagtt gcggtcaggc tacgaagtct gaatgtgcct tctattccgg tgaatttctt
      781 tcttccggtg cccggccaca acgtcgcaca tgcgtccact ctttccccgg attattgtct
      841 tcgcattctc tgtgtctatc gtatagcaaa tccatccgcg gagattcgca ttgcagccgg
      901 aagagaaatc cacctcaaag acaagcagca ggaagcattt cgcgtaggga attctctgtt
      961 tgtttcgggc tatctgaatg tgaagggatc ggatgcgcgt gaaacataca atttgattca
     1021 caacgcagga tttgaaatag atgcagacac atccgaactg gatcctgaat tccgcagctt
     1081 gatcatggga gaacaaaaca gtccagccgg agcgcagtca ggcgatcttc gcatgaaaac
     1141 acgcgaagac cttcgcagct tcgcagagta gaaaaacctg aattaagaaa ttcttttcag
     1201 aagcttctct gcctgttcct tgtatacgcc gtcttctttt tcgtgaccag ggttgagcgc
     1261 tgtatcctta caaaagttta agtgttccac tgccttgtct ttcttcccca tgtcgatgag
     1321 aagctccgcc agatagaggc gattattcat gaagttaggc gctagttgaa cgcacttttc
     1381 aagatgctcg aatgctttgt tcttatttcc aatgctgatg ggccagcctg gcgcctggtg
     1441 gtacaaacga ccgatcgcgc gatgaggtcc accaaagtaa aagttctcat tctctttcaa
     1501 gacaacttcc atggatttct tgaaaggatc aatggatgcc agagaagcaa gcgccccgcg
     1561 acacatcccc aggtaaccaa gattcgaagc ataccagaaa tttccataca cagcacgcgg
     1621 attcagcgtg attgcttcct ggccatattt aacgccgtta tcaaatgcat cttctcgttc
     1681 gcgcgttcct tccggcgaga acagtccttt atagaagaaa gcactggaga gcttcccccg
     1741 gatgatgtct ttttccggat tctccgcctt aagtttttcc aacaattcga tggctttctc
     1801 aagcgatccc gcagtttcgc gtgaatcaat cagtttgctt acttcttcca gcatgactat
     1861 ggactgaacc aatgccgaaa atttgaaagt taaaaaaact tactttacca aaaaaaggaa
     1921 gggggctacc tctgaacccc ttatgcaacc attcatcgcg cccggtttca atctacctct
     1981 cgcctggaca gcggatttgc tgtttcccgt tctaatcaag gcggtgcaga atatagacga
     2041 tgtggaaatt cgcccggaag acaaagagat gcttcgcacc ctgcgaaacg aacgcttgat
     2101 attttttaca aaccatccca cgactgcgga accacccatc gcatactaca ttggaaatct
     2161 catgggct
//
LOCUS       DAOQJL010000623         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_39544_length_2166_cov_3.475130, whole genome shotgun sequence.
ACCESSION   DAOQJL010000623 DAOQJL010000000
VERSION     DAOQJL010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39544_length_2166_cov_3.475130"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLG78_18860"
     CDS             complement(<1..1100)
                     /locus_tag="PLG78_18860"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE25242.1"
                     /translation="MFGCAIYLLGMAKGNLYAFWLSAIALVWLILLAVFARLSALRLR
                     FAIVSWQSRTLSARTRTEHQVSVSPAFTLPFFRLHYRIYGDFICGNSTTLFVSSESPA
                     EPDGICHIPFYFPLCGTLRAQGRLSVRDLFGFVRVILQEMPQRDVVVKPPLFAVPEAL
                     RVDVTSSPEASRKQQTADEDKYYMREYVPGDRMKDINWKASFRVMELITRISPVSPEP
                     SRLLRLELRPYSNQNENLLSLLHLNYVKSWMLSFLAAIQSRHPEYRFHVRAGAYEQFV
                     EDAQDLDVLNQALSTVHFQGEKQTGERVQDELFIFSTAFDASLPAYVASRSLAKTHLF
                     RTVTTGEKKGLTVQFFQGHRGLALPRISRAGR"
     gene            complement(1162..2127)
                     /locus_tag="PLG78_18865"
     CDS             complement(1162..2127)
                     /locus_tag="PLG78_18865"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE25243.1"
                     /translation="MSYIAAAKNGIDRLVDNIRQVIVVQPATLETILASKIAGGHVIL
                     ADAHGVGKTSLARALSGSIDWGEGQTKDGVKIEPFSRIQCTVDLLPQDILGFNRFDMQ
                     SNRTTFHSGPIFANFILCDEINLLTPKTQGSFLQVMEEQRITIEGSTYHLPDPFFIIA
                     TMNLKGSHLFPLPAPQLDRFMVQISLGYPSEQDEIQILTKHGKDDSWKDFKPVIDPNE
                     LLAWQKLVDQVSLHADVASYIVRIIRQTRKHPGVITGASPRSGIKLSRLVRALALVRG
                     SDYVTPDLVKEMAFPALCHRIELEDPSRSPSSVIQEVLKEVPAPG"
BASE COUNT          564 a          521 c          583 g          498 t
ORIGIN      
        1 atgcgccctg ctcgcgaaat cctgggaagt gctagccctc tatgcccctg aaagaattga
       61 accgtaagtc ctttcttctc acctgtagtg accgtgcgaa acagatgagt cttcgcgaga
      121 ctccgcgacg ccacgtacgc aggaagtgaa gcgtcaaatg ctgtgctgaa gataaagagt
      181 tcatcctgga ctctctcacc cgtttgcttt tctccctgga aatgaactgt gctcaacgcc
      241 tgattcaaaa cgtctagatc ctgggcatcc tccacaaact gctcgtatgc tccggcgcgc
      301 acatgaaatc tatactcagg gtggcgagac tgaatggctg cgagaaaaga aagcatccaa
      361 cttttgacgt agttgagatg aagaagggag agcaaattct cgttctggtt tgaatagggt
      421 ctcagctcca acctcaaaag cctggaaggt tcgggggaaa cgggagaaat acgagtgatg
      481 agctccatta cgcgaaaaga agccttccag ttgatgtctt tcattcgatc gcctggaaca
      541 tactctctca tgtagtactt gtcttcgtct gcagtctgtt gcttgcgact tgcctccgga
      601 ctgctggtca catccacgcg aagggcttct ggaacggcaa acaggggtgg tttgaccaca
      661 acgtcccgct gcggcatttc ctggagaatc actctgacaa agccaaacag atcgcgcacg
      721 gagagccttc cctgtgcgcg aagggtgccg cataacggga agtagaatgg gatatgacag
      781 attccgtccg gctctgccgg gctttcggag gatacaaaaa gagtggtaga attcccacaa
      841 atgaaatcgc catagattct ataatgcagg cgaaagaaag gaagagtgaa cgcaggagac
      901 acagatacct ggtgttctgt cctggtgcga gcagatagag tgcgggattg ccagctaaca
      961 atcgcgaagc gcagacgaag ggcactcaat cgcgcaaaca cggcgagcaa tatcagccac
     1021 accagagcaa tggcagaaag ccagaacgcg tacaggttcc cctttgccat tccgagcaaa
     1081 taaatcgcac agccaaacac cagagtgcca ggtccggtga atggatagat gcgggaaatg
     1141 aacttgaaga tgctgcgtat tctagccagg agccggaacc tccttcaaca cttcctgaat
     1201 gacagaggac ggagagcgtg aaggatcttc caattcgatg cggtggcaga gcgcggggaa
     1261 tgccatttct ttgaccaggt ccggtgtaac gtaatcgctg ccacgcacga gtgcaagagc
     1321 acgcacaaga cgactgagct tgatgccaga acgcggactt gcgcctgtaa ttacgcctgg
     1381 atgttttctg gtctggcgga tgattcgaac aatataagaa gcgacgtctg catgcagaga
     1441 aacttgatcc acgagtttct gccacgcgag gagttcgttt ggatcaatga caggtttgaa
     1501 atctttccag gaatcgtctt tgccgtgttt ggtgagaatt tggatctcat cctgttcgga
     1561 aggatagccc agagaaatct gaaccataaa acggtccagc tgcggtgcgg gaagcgggaa
     1621 caagtgactt cccttcaggt tcatggtagc aatgataaaa aatggatccg gaagatgata
     1681 cgtgcttcct tcaattgtaa tgcgctgctc ttccatcact tgaagaaagc ttccctgagt
     1741 tttgggagtc agtagattga tttcatcgca gagaatgaag ttcgcgaaaa ttggaccgct
     1801 gtgaaacgta gtgcgattgg actgcatgtc aaatcggttg aagccaagaa tgtcttgtgg
     1861 aaggagatcc acagtgcact ggatacggct gaatggttca atcttgactc cgtcttttgt
     1921 ctgcccttct ccccagtcta tggaaccaga aagagcgcgc gcaagtgaag tcttacctac
     1981 accgtgcgcg tctgcgagaa tgacatgacc cccggcgatc ttggatgcaa gaatcgtctc
     2041 gagggttgcc ggttgtacga cgatgacctg gcggatattg tcgaccagcc ggtcgatgcc
     2101 attcttggct gcagcaatgt acgacatgct gcgcttactt cagcttgtat ttctttttga
     2161 aacgat
//
LOCUS       DAOQJL010000624         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_40083_length_2150_cov_4.199523, whole genome shotgun sequence.
ACCESSION   DAOQJL010000624 DAOQJL010000000
VERSION     DAOQJL010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40083_length_2150_cov_4.199523"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLG78_18870"
     CDS             <1..563
                     /locus_tag="PLG78_18870"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNE25244.1"
                     /translation="SVETRICRWKRGDLLSGYIRQRVLGPILDLLKRGVTPHKLALCI
                     AAGAMLGIFPVIGATMALCVIVGYLLRLNAIAIQAVNYAVYPLQIAMLVPFVRMGEWI
                     TGSDPFPVSPSAVLAMLQEGIVKTLVHFSSAIIHAVLAWSLLCLPAGLLIYLAFLPIL
                     RKSAEHYARLRARHAESTDDQLQAKP"
     gene            complement(548..>2150)
                     /locus_tag="PLG78_18875"
     CDS             complement(548..>2150)
                     /locus_tag="PLG78_18875"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE25245.1"
                     /translation="SVWFDLSVRNDSARDLLYLSIPVPVLDHLELYERNGESWTVQKS
                     GDLYTREERNDFRNPTFSLSIKPGETRRYLLRIITTSAIPFHAVVSTDLAITAESESE
                     MVWTAGYFGLLTAMILYNLFIYFSIRERSYLAYSVYVILLWMFQACNYGLAAQYILPN
                     HPVIANYAYPILASLLALSTVIFTYTFHNVHVRFLKLRKVFWATGVVCILSIGATFYV
                     QNRFNNALSIAISVLVLYTSVLSLMRGYRPALFFLAAWTGFVILTTLWSLSIRGIIQN
                     FLIFRYAFLVGSATEVVILSLALGDRINLLDSEKRAALAEQIQLKTRLLDAFSRFVPR
                     QFLRSLGKESPEEVNLGDASAAEVTILFSDIRGFTGMSERMNVTENFNFLNSYLKRME
                     PVIESRGGFVDKYIGDAIMAIFLGNPEHAIHAAEDMMKALEEYNAHRASQNFEPIKIG
                     IGIHRGPVMLGTVGSQKRMDTTVIGDSVNACARIESLTKKIAPVLISEDALAGVSDQI
                     RNKLKRIARVQVRGKTQSLVLYGFA"
BASE COUNT          524 a          520 c          556 g          550 t
ORIGIN      
        1 tatccgtaga aacacggatc tgccggtgga agcgcggtga tctcttgagt ggctacattc
       61 gacaaagggt gctcgggccc attctggatc tgctcaagag aggggtgacg cctcataaac
      121 ttgcattgtg cattgcagca ggcgcgatgc ttggaatctt ccctgtgatt ggtgcgacca
      181 tggcgctttg cgtcatcgta gggtatctac tccggttgaa cgcgatcgcc attcaggccg
      241 tgaattacgc agtataccct cttcagatcg ccatgctcgt tccttttgtg cgaatgggag
      301 agtggatcac aggctcagac ccgtttccag tttcgccttc tgcggtgctt gccatgttgc
      361 aggaaggaat tgtgaaaact ctggtccatt tctccagtgc gatcattcat gcagtacttg
      421 catggagcct cctttgtctg cctgcgggat tgctcatcta tctcgcgttt ttgccaatcc
      481 ttcgcaagag cgcagaacac tacgcgaggt tgcgcgctcg ccacgcggaa agcactgacg
      541 accagcttca ggcgaagccg taaaggacta gagactgagt tttcccacgc acctgcacgc
      601 gcgcaattcg cttgagttta ttcctgatct ggtcagatac acctgccagt gcgtcctctg
      661 aaatcaaaac aggtgcgatc ttcttggtca gactctctat tcgcgcgcat gcgttgactg
      721 aatcgccgat cacagttgta tccattctct tttgcgaacc gactgttcca agcatcacag
      781 gaccgcgatg aattccgatt ccaatcttga ttggctcaaa gttctggctg gctctgtgcg
      841 cgttgtattc ttcgagtgct ttcatcatgt cttccgcggc atggatggca tgctccggat
      901 ttccaagaaa gatcgccatg atcgcatctc caatatactt atccacgaat ccgccccggc
      961 tttctattac gggttccatt cttttgagat agctattgag gaaattgaag ttctccgtca
     1021 cattcattct ctcagacatt ccagtgaaac cgcgaatatc ggagaacaga atggtgactt
     1081 ctgctgcact cgcatctccg aggttcactt cttcggggct ttccttgccc agagaccgta
     1141 agaactgccg cggaacaaaa cgagagaacg catccagaag ccttgttttt aactgaattt
     1201 gttctgccag tgccgctctc ttttcagagt cgagcagatt gatgcgatcg cccagagcca
     1261 gagacaggat cacaacttct gttgcagaac ccacaaggaa tgcgtagcga aagatcagga
     1321 aattctgaat gataccgcga atggagagac tccacaatgt agtgagtatt acaaatccag
     1381 tccaggcggc caggaagaac agtgctggtc tgtacccgcg cattagactg agaacagatg
     1441 tatagagaac cagaacgctg attgcaatgg acagagcatt gttgaatcga ttctgcacat
     1501 agaaagtggc gcctatgctg aggatgcaga ctacacccgt ggcccagaat acttttcgca
     1561 gcttcaggaa gcgaacgtgc acattgtgaa acgtatatgt gaagatgaca gtggagagtg
     1621 cgagtaaaga agcgaggatg ggataggcgt agttcgcgat gactggatgg ttgggcaata
     1681 tatactgtgc tgccagtcca tagttgcatg cctgaaacat ccacagtaag atcacataga
     1741 cagagtaagc caggtaggat ctctctcgga tggagaagta gatgaacaaa ttgtacagta
     1801 tcatcgcggt gagcagacca aagtagcctg cagtccagac catttcggat tcagactccg
     1861 ctgtgatggc aagatccgtg gaaaccactg catgaaacgg gatcgcgctt gttgtgatga
     1921 tgcgaaggag gtagcgtctg gtttctcctg gttttataga tagagagaaa gtggggttgc
     1981 gaaagtcgtt tcgttcttct cttgtgtata gatcgcctga tttttgtacg gtccacgatt
     2041 ctccgtttct ctcataaagt tccagatgat ccagtactgg cactggaatg ctgaggtaga
     2101 gaaggtctcg tgcggaatcg ttacgaacgc tcagatcgaa ccacacagat
//
LOCUS       DAOQJL010000625         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_40521_length_2137_cov_4.280019, whole genome shotgun sequence.
ACCESSION   DAOQJL010000625 DAOQJL010000000
VERSION     DAOQJL010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40521_length_2137_cov_4.280019"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLG78_18880"
     CDS             <1..803
                     /locus_tag="PLG78_18880"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNE25246.1"
                     /translation="EVKFFSEFGKLHPLVQKLEKKAIHQCGTLGGGNHFIELCLDTEQ
                     RVWLMLHSGSRNIGKTLAELHISIAKKLIHNQKLPDRDLAVFLSGTPEMEAYRRDLYW
                     AQRYAFLNRETMFEIYQDVMREFFPSVKFQRPIMCHHNYVAEEVHFGEKVFVTRKGAI
                     RAGRGEMGIIPGSMGTRSFIVRGLGHPDAFESASHGAGRRMSRTQAKRTFTEKDLREQ
                     TSGVECRKDHGVLDEIPGAYKDIAQVMADQSDLVGIVHELKQVLCVKG"
     gene            883..>2137
                     /locus_tag="PLG78_18885"
     CDS             883..>2137
                     /locus_tag="PLG78_18885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE25247.1"
                     /translation="MNPVDCKVLVIEDNPVVTQAIRSILEERGYEPVCRSDGSSGLEA
                     AGQEDPDVILCDINLPDMDGFRILRSVQQDPRLQHIPFIFLTSRGGAEDLREGMMSGA
                     DDYLSKPFHPDELIASIQARIRRNQVIERRYRENFAMQEQRLIFSASFDETTELPLEP
                     LLKAEYETFARDENGYLVIARFPGLERIAPMVAADDVRCIIRAISERLMTGTGSAVFR
                     IGRDELAFFVLNADEAGASVHAEQCLRLFHSCLECNERLLHLPGLFGMSQLKHDVPFE
                     SALRNARVAAKYAEASGGTGFRIFDESMETRLGDQLFLESELRQALARNEFHLVFQPR
                     VLLPGRVVGAEALVRWTHPRLGNVPPSVFIPVAEESGTIDQIGNWVLSESAKKLSEWK
                     MQGLPEDFTLAVNVSGRQLESADFLP"
BASE COUNT          536 a          479 c          595 g          527 t
ORIGIN      
        1 cagaagttaa gtttttttcc gaatttggaa agcttcatcc cctggtgcag aaactggaaa
       61 agaaagcaat tcatcaatgt ggcacactgg gtggcggaaa ccattttata gagctttgtc
      121 tggatacaga gcagagagtc tggttgatgc ttcattctgg atcgcgtaac attggtaaga
      181 cgctagcgga actacacatt tccattgcaa agaagctgat tcacaatcaa aagctgccgg
      241 atcgagatct ggcggttttc ctctcgggaa caccggaaat ggaggcgtac agacgtgacc
      301 tttactgggc gcagagatat gccttcttga acagggaaac catgtttgaa atttatcagg
      361 atgtgatgcg tgaattcttt ccgagcgtaa agtttcaaag accaatcatg tgtcatcaca
      421 actatgtagc cgaggaagtc cattttggtg agaaggtttt tgttacgcga aagggcgcca
      481 tccgtgcagg acgtggagaa atgggaatca ttccggggtc catgggaaca cgatcattca
      541 tagttcgagg gctgggccat ccagatgcct tcgaaagcgc ttcgcacggt gcaggtcgtc
      601 gcatgagcag aactcaagct aagcgcactt tcacggaaaa agatttgcgc gaacaaacca
      661 gcggagtgga atgcagaaag gatcacggag tactggatga aattcctggc gcgtacaaag
      721 atatcgcaca ggtgatggcg gatcaatcgg atctggtggg aatcgttcat gaactgaaac
      781 aggttttgtg cgtgaagggc taaagtcgcg tacgtagaag tactcaaaaa aatagtcgca
      841 ctacctattg aaactaatct tcccgcattg taggatgtgg gcgtgaatcc ggtagattgc
      901 aaagtgcttg taatcgaaga caatcctgtg gttactcagg cgattcgatc catccttgaa
      961 gaaagaggtt acgaacccgt ctgcagatca gacgggtcga gtggacttga ggctgcgggt
     1021 caagaggatc cagatgtgat tctgtgtgac atcaatctgc cagacatgga cggttttaga
     1081 atcctccgtt ccgtgcagca ggatcccagg cttcagcata ttccgtttat ttttctcacg
     1141 agccgtggag gcgcggagga tttgcgcgaa ggaatgatgt ccggtgcgga cgattacttg
     1201 agcaagccgt ttcatccaga tgaactcatt gcctctattc aggctcggat cagaagaaac
     1261 caggtcatcg agagaagata tcgcgagaat ttcgcaatgc aagaacagag gcttattttt
     1321 tccgcaagct tcgacgaaac tacggagctt ccactggagc cacttctaaa ggcggaatac
     1381 gaaactttcg caagagacga gaatggatac ctggtgatcg ccaggtttcc tggtctcgaa
     1441 agaattgcgc ccatggtagc tgcagatgat gttcgctgca tcattcgagc tatttcggaa
     1501 cggttgatga ctgggacggg ttccgcagtg tttcgcatag ggcgcgatga gcttgcattc
     1561 tttgtcctga atgcggacga ggctggggcc tcagttcacg cagaacaatg tcttcgcctc
     1621 tttcactctt gccttgagtg caacgagcga cttcttcatc tgcccgggct gttcggtatg
     1681 tcccaactca agcacgatgt gccattcgaa agcgcgctga gaaatgcacg cgtcgcggca
     1741 aagtatgcgg aagcgtcggg tggaacaggc tttcgcattt ttgatgaatc catggagact
     1801 cgcctggggg accaactatt tctggagagt gaattgcgtc aggctctcgc gcgaaatgaa
     1861 tttcatctcg tttttcagcc tcgtgttttg cttccgggtc gcgtcgtagg cgcagaagcc
     1921 ctcgtgcgct ggacccatcc caggctgggc aatgttcccc cgtcggtttt tattccagtc
     1981 gcggaagaat ccgggaccat tgatcaaatc ggaaactggg tgctttcgga gagtgcgaag
     2041 aagttatccg aatggaaaat gcaaggtttg ccggaggatt ttactctggc agtgaacgtc
     2101 agcggccgcc agcttgaaag tgccgatttt ctgcctc
//
LOCUS       DAOQJL010000626         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_40672_length_2133_cov_4.264678, whole genome shotgun sequence.
ACCESSION   DAOQJL010000626 DAOQJL010000000
VERSION     DAOQJL010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40672_length_2133_cov_4.264678"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLG78_18890"
     CDS             complement(<1..384)
                     /locus_tag="PLG78_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE25248.1"
                     /translation="MKLWDTINKPITRKAFLATFAGAFYTAAVERYWIAVNEYNISFP
                     RLPRQFDGFRIGFLSDIHLGLFMPGAWVDAIVDRANALGVDALVLGGDYVHARKTDHE
                     LLLVWSKLKKLSAPSGVFAVNGNHDH"
     gene            complement(381..935)
                     /locus_tag="PLG78_18895"
     CDS             complement(381..935)
                     /locus_tag="PLG78_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE25249.1"
                     /translation="MRKLIFGINITADGHCSHTDGIADEELHEYFTELLRATDTILYG
                     RVTYQLMVPFWPDVAKNQSMSRTSNEFARVFDSLDKIVFSRTLKQAEGNARIVRENLE
                     GEVIALKQKPGKPICVGSLSIASQLSRAGLIDEYHFVVQPVVAGKGPRLFDTIKQDQS
                     IPLELLESKRFAQGQMALHYRKRT"
     gene            complement(954..1475)
                     /locus_tag="PLG78_18900"
     CDS             complement(954..1475)
                     /locus_tag="PLG78_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25250.1"
                     /translation="MKNQTKILLCFSFLSLTGCSHVVAAFIVNFQDSPATVQFWSVTT
                     PGRQCFGELQLLPLVDGTFSPDSVYSSASQIDLKTEGDHRTIALPARTALLLDRELNR
                     WDLHCVAQISKLKIITPQGTIEYTDGEVTRAFERTRAHSDSFILSIRPDARKQSFRSR
                     KDPLASHTWRIDG"
     gene            complement(1485..1736)
                     /locus_tag="PLG78_18905"
     CDS             complement(1485..1736)
                     /locus_tag="PLG78_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25251.1"
                     /translation="MKQIIFALLLPLFTVSSCGSTNAECKKAKQQALQFATAFCLAMA
                     STYTQSSYYTPGSYFASCWLEFGGLSGMGSCSTTSSSKD"
     gene            complement(1813..>2133)
                     /locus_tag="PLG78_18910"
     CDS             complement(1813..>2133)
                     /locus_tag="PLG78_18910"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE25252.1"
                     /translation="LNGLIDLYTEDAVNHQVVTEPLVGRDSIARMFKIEFGRAKMVCI
                     PEHIHEAGDWAILEWKDPLGLRGCGFFQIRDGRIAFQRGYFDQLTFFKLQGIPVPSEY
                     LGSE"
BASE COUNT          502 a          527 c          566 g          538 t
ORIGIN      
        1 gtgatcgtga ttgccgttca ccgcaaacac tccggaggga gcgctcagtt ttttcagttt
       61 ggaccacacg agcaatagct catgatccgt cttgcgcgcg tgaacatagt cgccacccag
      121 gaccagtgcg tctaccccca gtgcgttggc gcggtctacg attgcatcca cccaggcccc
      181 tggcatgaag agtcccaggt gaatgtccga tagaaagcca attctaaatc catcgaactg
      241 tcggggcagg cggggaaatg aaatgttgta ttcattgacc gcgatccagt agcgttcaac
      301 ggcagcagtg taaaatgcac cagcaaaagt tgccagaaat gcttttctgg taatgggttt
      361 gttgatggtg tcccagagct tcatgttcgc tttctataat gaagggccat ctgcccctga
      421 gcaaatcgtt tgctctccag tagttccagg ggaatcgatt gatcctgttt tatagtgtca
      481 aacagccgcg gtcctttgcc cgcaaccaca ggttgaacca caaaatgata ttcgtcaatg
      541 agtccggctc gcgatagctg agatgcgatg ctgagactgc ccacacagat tggcttacct
      601 ggtttttgtt tgagtgcgat cacttcccct tcgaggttct cacggacgat tcttgcattt
      661 ccttctgctt gcttcagggt gcgcgaaaat acaatcttgt ccagagaatc gaaaacgcgc
      721 gcgaattcat tggatgtcct ggacatagac tggtttttgg ccacgtcagg ccagaacgga
      781 accataagct ggtatgtgac gcgaccgtac aggatggtgt ccgtcgctcg cagaagctct
      841 gtgaagtatt cgtgcaactc ttcgtctgcg attccgtctg tgtggctgca gtgtccatct
      901 gctgtgatgt tgattccaaa aatcagcttt cgcatgaatt tctccggttg cgttcaacca
      961 tcgatccgcc aggtgtgact ggcaagagga tctttcctgc ttctaaagga ttgcttacgc
     1021 gcgtccgggc gtatggatag aatgaatgaa tcactgtgcg cccgcgttct ctcaaacgcc
     1081 cgcgttactt ctccatctgt gtactctatg gttccctgag gtgttatgat tttgagtttg
     1141 gaaatttgcg cgacgcaatg gagatcccac cggttcaatt ctcgatcgag aagaagggca
     1201 gtacgcgccg gcaacgcaat cgttctgtga tctccttcgg ttttcagatc gatctgcgat
     1261 gcagacgaat agacagagtc cggggaaaag gttccgtcca cgagcggaag gagttgaagt
     1321 tcaccaaagc actggcggcc cggggtggtg acagaccaga actggacggt tgcaggcgag
     1381 tcctgaaagt ttacaatgaa tgctgccact acgtgcgaac atcccgtcag ggataaaaag
     1441 gagaaacata ggaggatctt tgtttgattc ttcaaggtcc aatctcagtc tttgctcgaa
     1501 cttgttgtgc tgcaggaccc catgccgctg agtcctccga attccagcca gcaagacgcg
     1561 aaataagatc ctggtgtgta ataggaggac tgggtgtagg tggaagccat ggccaggcag
     1621 aaagcagttg cgaattgcag tgcttgctgt ttggcctttt tacattctgc attcgtggaa
     1681 ccgcaactcg ataccgtaaa gagaggcaag agcaaggcga aaatgatctg tttcatggaa
     1741 atcctcctgt ccaggaatgg tgttctctat gcgagcgagt cagattgtcg cctgacggca
     1801 aataagcttc ggctactctg agcccaggta ttcagaaggg actggaattc cctgcagttt
     1861 gaaaaacgta agttgatcga aatagcctct ttgaaaagca atgcgaccat ctcgaatttg
     1921 aaagaagccg cacccgcgca gacccagggg gtctttccat tccagaatcg cccagtcacc
     1981 ggcctcgtgg atatgctctg gaatacagac cattttcgcg cggccgaatt caatcttgaa
     2041 catgcgcgca atggagtcgc ggcccacgag cggttctgtg actacctgat gattgaccgc
     2101 atcttctgtg tacaggtcta tcaggccgtt tag
//
LOCUS       DAOQJL010000627         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_40983_length_2124_cov_4.016433, whole genome shotgun sequence.
ACCESSION   DAOQJL010000627 DAOQJL010000000
VERSION     DAOQJL010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40983_length_2124_cov_4.016433"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLG78_18915"
     CDS             complement(<1..637)
                     /locus_tag="PLG78_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25253.1"
                     /translation="MIRDIFLRSSPGLALLFLLFFAECSGRDRKFQQIQDLIQAGNRQ
                     EAEDMIKAELLEENRKHAKEFDDGSLEAPLASPGARTAVWVKGESKLVILRNGKRNEV
                     SLGRRIKNIQLAPGGKFALILVIEGQGCKLAAVDLLEREVIHEISSCDPGAAITGDGQ
                     EILYSAQGGVGRIHLKNREKTVEDVLLEKSSFAAHYPKLTNRYYVVPDAQNH"
     gene            684..>2124
                     /gene="aspS"
                     /locus_tag="PLG78_18920"
     CDS             684..>2124
                     /gene="aspS"
                     /locus_tag="PLG78_18920"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNE25254.1"
                     /translation="MSGKAVQNWIHQVFTKRQPCGEPGDAGKKVYLAGWAFRYRDQGG
                     LIFVDLRDRSGIVQVVFDRSSMGDSFEQATHIRSEAVLAIEGKIRKRAPEAVNAKIPT
                     GTIEVVVEKFEVLNVSRPLPFQLDEFTEAGEEHRLKYRYLDMRREEMRDTLITRSKLN
                     QCIRQYLEKDGFIEVETPILIKSTPEGARDFLVPARLSPGRFYALPQSPQLFKQILMV
                     GGLEKYFQIVKCFRDEDLRADRQPEFTQLDMEYSFFDEDMIVESLEGMWAHVLKKVFG
                     VKVKTPFPSITYHDSMEKYGNDRPDLRFGMELVDIADIAMKSSFQVFQKAVEAGGRVK
                     ALCVPGGGVLSRKDIDDLTTWVGQDFGAKGLAWMKHEADGLKSAVSKFFTPELLSEIS
                     KRLKTKEGDIVFFAGDRAHIVHNTLGNVRLRLAKQLNLIPEDEWNFVWVRDFPLFDRD
                     VDTGALNSVHHPFTAPNPEDIPILKDPARF"
BASE COUNT          532 a          517 c          534 g          541 t
ORIGIN      
        1 gatgattctg tgcgtctgga accacgtagt atcgattcgt gagtttggga tagtgcgcag
       61 cgaaacttga cttttccaga agcacatctt ccaccgtctt ctccctgttc tttaaatgaa
      121 tgcgaccgac tccgccctgc gctgagtaca ggatttcctg accatctcct gtgattgcag
      181 ctcccggatc gcacgatgag atttcgtgaa tcacttcacg ttccagcaag tccaccgcgg
      241 cgagcttaca accctgacct tctatgacga gaatcagcgc aaattttcct cccggtgcaa
      301 gctgaatgtt tttgattctt ctgcccaggc tcacttcatt tctttttccg ttgcgaagga
      361 tgacaagctt ggattctcct ttgacccaga ccgcagtgcg tgcgcctgga ctggccagcg
      421 gcgcttcgag agacccgtcg tcgaattcct ttgcgtgctt gcgattctct tcgagcagtt
      481 ctgctttgat catgtcttct gcttcctggc gatttcctgc ctggatgaga tcctgaatct
      541 gctggaactt ccgatcgcga cctgagcact cagcgaaaaa caaaaggaac agcagcgcca
      601 ggccagggga agagcgaaga aaaatgtctc gaatcatgac gatgagacga agtaaaacaa
      661 attgacgccc tttacaagca ggaatgtctg gaaaagccgt gcagaactgg attcatcaag
      721 tttttacaaa gagacaaccc tgcggcgagc ccggcgatgc cgggaaaaaa gtctatctgg
      781 ctggctgggc gtttcgttat cgcgatcaag gtggcttgat ctttgtcgat ctgcgtgatc
      841 gatcggggat tgttcaggtc gtgttcgacc gcagttccat gggagattcg tttgaacagg
      901 ccactcacat tcgttcggaa gccgttcttg ctatagaagg taagattcgc aaacgcgcgc
      961 ctgaggcagt gaatgcgaaa atccccaccg gaaccataga agtagttgta gaaaaatttg
     1021 aagtgctcaa tgtttcgcgc ccgcttccat tccagctcga tgagtttacg gaagcaggag
     1081 aagagcacag actcaagtat cgctatctgg acatgcgtcg cgaagaaatg cgcgatactc
     1141 tgattacaag gtccaaactc aatcagtgca ttcgtcagta tctggaaaaa gacggcttca
     1201 ttgaagtaga aactcccatt ctgatcaagt ctactccgga aggagccagg gacttcctcg
     1261 ttccagctcg tttatcccca gggcggttct atgcattgcc tcaatctcct cagctgttca
     1321 aacagattct gatggttggt ggccttgaga aatactttca aattgtaaaa tgctttcgcg
     1381 atgaagacct tcgcgcggac agacaaccag agttcactca gctggacatg gagtacagtt
     1441 tcttcgacga agacatgatt gtagaatcgc tcgaaggaat gtgggcacac gtgctcaaga
     1501 aagtattcgg agtgaaagtg aagactccgt ttccttctat tacataccac gactccatgg
     1561 aaaaatacgg caacgacaga ccggacctta gatttggaat ggagctcgta gacatagctg
     1621 acattgcaat gaaaagctcg tttcaggttt ttcagaaggc tgtagaagca ggtggaagag
     1681 tcaaggctct ctgtgttcca ggcggcggag tgctttcgcg caaggacata gacgatctaa
     1741 ccacctgggt cggccaagat tttggtgcaa agggccttgc gtggatgaag cacgaagcgg
     1801 atggactgaa atcagccgtc tccaagttct ttactcctga acttctttct gagatttcta
     1861 agcgactcaa gacgaaagaa ggagacattg tgttttttgc gggagatcgc gcgcacattg
     1921 tgcacaacac tcttggcaac gttcgacttc gcctggcaaa gcaattgaat ttgattcctg
     1981 aagatgaatg gaatttcgtg tgggtgcgag actttcctct ctttgatcgg gatgtggata
     2041 caggtgctct caattcagtg caccatccat ttactgcgcc caaccccgaa gacattccga
     2101 ttctaaaaga tccagcgcgt tttc
//
LOCUS       DAOQJL010000628         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41016_length_2123_cov_3.760155, whole genome shotgun sequence.
ACCESSION   DAOQJL010000628 DAOQJL010000000
VERSION     DAOQJL010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41016_length_2123_cov_3.760155"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLG78_18925"
     CDS             complement(<1..426)
                     /locus_tag="PLG78_18925"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNE25255.1"
                     /translation="MHTLRLQLTSARISVLAKRELSSLDNSDEQDLRLGELEAARLIT
                     GKKVFSLKQVHGTHAVQVEDKSQQTGIYFAEADAVFTSRKDVALMVRTADCLPLFFEG
                     RGEETWVGVIHAGWRGLAAGIIRSTLSRVIELGKIATLKI"
     gene            484..1194
                     /locus_tag="PLG78_18930"
     CDS             484..1194
                     /locus_tag="PLG78_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNE25256.1"
                     /translation="MNTQSYRRRLLRVSQSRPVVKICGNVYFEDSLMVAAARPDLMGW
                     IFSPHSKRRVSIESAARQIECIQRESPDIFHVAVCAGNSIPDILRIARAIPGFDFVQI
                     VDGPGVASEMRTHLRANMEVIPVLRVSSPIVDQDLSIVMPADWVILDAFSPHALGGTG
                     IAFDTAWVQNVSHRYILAGGLTPANVRERLLNSGASGADVSSGVEAETGLRTGPGRKD
                     PQKVVSFIENVKAISFNR"
     gene            1191..>2123
                     /locus_tag="PLG78_18935"
     CDS             1191..>2123
                     /locus_tag="PLG78_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE25257.1"
                     /translation="MSQDAALKDALDTPVMRQYLDLKKMHPESILFFRMGDFYEMFLD
                     DAISAAPILDVALTRRQGVVPMAGIPYHSVDSYLARLIAAGRRVAIAEQAVDEENPKL
                     MRRLVTRVVSPGTVIEESLLPESAHSYLAAVALSQDETICGIALSDITTSDFRAYEVS
                     TREAAGFLLKIGPAEILTTAHSRSVVATPGLPPAVILEEWKGQAEEGARQMERIFHMK
                     ARSLDFADSSPACGACSLVIHYLMSSFPTGSFPLPAPVFMRGDEDSALLDEETVRNLE
                     LTRNQRGTEERTLFSVLNRCRTTAGRRLLRERLLE"
BASE COUNT          472 a          553 c          576 g          522 t
ORIGIN      
        1 aatcttcaga gtcgcgatct tgccaagctc aatgacgcgc gaaagcgtac ttcgaatgat
       61 tcctgcagcc aggcctctcc agcccgcgtg gatgacaccc acccaggttt cctcacctct
      121 gccctcgaag aaaaggggaa ggcagtctgc tgtgcgaacc ataagcgcca cgtctttgcg
      181 agaggtaaac actgcgtccg cttccgcaaa gtagattcca gtctgctgtg atttgtcttc
      241 cacttgcaca gcatgcgtcc cgtgcacttg tttgagagag aatactttct tgcccgtgat
      301 gagtcttgcg gcttcgagct ctcccaggcg aagatcctgc tcatccgaat tgtccagaga
      361 tgatagttcg cgttttgcga gtacagaaat tcttgcagac gtcagctgca gccgcaatgt
      421 gtgcatgtta gattccagcg gttcaatttg cggtaagcag gctctggatc aatcagaata
      481 aaaatgaaca cccaaagcta tcgccgtcgc ctgctgcgcg tctctcagtc tcgacctgtt
      541 gtgaagatct gtgggaacgt atactttgaa gacagtttga tggtcgcagc cgcgcggccg
      601 gatctgatgg gatggatttt ttctccccat tcgaagcgtc gcgtgtccat cgagtctgca
      661 gctcgacaga ttgaatgcat ccagagagaa agtccggaca tttttcatgt ggccgtttgc
      721 gccggaaact ccattcctga tatccttcgc atcgctcgcg ccataccagg ttttgatttt
      781 gtgcagattg tagatggtcc cggagtggct tccgaaatgc gcactcacct gcgtgcgaac
      841 atggaggtca ttcctgttct tcgtgtttct tctcccattg tagaccagga cttgagcatt
      901 gtgatgcccg cagactgggt gattctggat gccttttccc ctcacgctct gggcggaacc
      961 ggaatcgcat tcgacaccgc ctgggtgcaa aatgtatctc acagatacat tctggcgggc
     1021 ggactgacgc cggctaacgt tcgcgaaagg cttctgaatt cgggtgcttc aggagcagat
     1081 gtgagttcgg gtgtagaggc agaaacggga ctacgaactg gccccgggcg aaaggatcca
     1141 caaaaggtgg tttctttcat agaaaacgtg aaggcaattt cttttaacag atgagtcagg
     1201 acgcagcact caaggacgca ctcgatacgc cagtcatgcg gcagtatctg gatttgaaga
     1261 aaatgcatcc agaaagcatt ctcttctttc gcatggggga cttctacgag atgtttctgg
     1321 acgatgcgat ttcagcggct cccattctgg atgttgcttt gaccagacgc cagggcgtcg
     1381 tgcccatggc aggaattccc tatcatagcg tggattcgta cctggcacgg ttgattgcag
     1441 ccgggcgtag ggtcgccatc gccgaacagg ccgtagacga agaaaatcca aaactcatga
     1501 ggcgactggt gacgcgtgtc gtgtctccgg gaactgtgat cgaggaatct ttgcttccag
     1561 agtcggcaca ttcttacctg gccgcggttg ctctaagtca ggacgaaacg atatgcggca
     1621 ttgctctttc tgacatcaca acttctgatt ttcgcgcgta cgaagtgtcg acccgggagg
     1681 cagccggatt ccttttgaag attggcccgg cagaaatcct gacaacggct cattctcgct
     1741 cggtggtggc aacacccgga ctgcctcccg ctgtgatatt ggaggaatgg aaaggccagg
     1801 cggaggaagg tgccaggcaa atggaacgca tctttcatat gaaggcgcga tcgcttgact
     1861 ttgcggattc ttcccctgcc tgcggagcct gttcccttgt gatccactat ctgatgtcca
     1921 gctttcccac aggaagcttt ccacttccag cacccgtttt catgcgcggt gacgaagatt
     1981 ccgctcttct ggatgaagaa acagtgcgca atctggaatt gaccagaaac cagagaggga
     2041 ccgaagaacg gaccttgttt tccgttctca atcgatgcag gactacagca ggaaggcgac
     2101 tactcagaga gcgtttgctt gag
//
LOCUS       DAOQJL010000629         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41409_length_2112_cov_3.571706, whole genome shotgun sequence.
ACCESSION   DAOQJL010000629 DAOQJL010000000
VERSION     DAOQJL010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41409_length_2112_cov_3.571706"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLG78_18940"
     CDS             complement(<1..302)
                     /locus_tag="PLG78_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25258.1"
                     /translation="MKKIAWISLVIVACFASQSCRSSQLAFTEDTTKYNGMVPVDGPS
                     GIMQKTTVIGVHLLFGLIPVYRASLDSALDSFTKDARKRGASRVRVISATRETGIW"
     gene            complement(352..987)
                     /locus_tag="PLG78_18945"
     CDS             complement(352..987)
                     /locus_tag="PLG78_18945"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE25259.1"
                     /translation="MIAFFDLDGTLEDSRQDMALSINRVRETKSLPALSLEVARSLVN
                     RGMDALTEAAFPEIIENQTDLKKVRALYEEDYLSHVVDHTRLYDGISSMLQGVASIGR
                     LVVYTNKPERISRELLKRLQVLETVSGIIGGDSFPESKPSPIPMQTMADRLGETGARW
                     MTGDTAADMQAARNSGARSIWASWGYISSVPEPSPDFTAKNPSDVLELIRN"
     gene            complement(984..1367)
                     /locus_tag="PLG78_18950"
     CDS             complement(984..1367)
                     /locus_tag="PLG78_18950"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE25260.1"
                     /translation="MPPGVRVVKWPHKETPDEESVKKEMAGLGYRVYDLQTIPPWFER
                     SRHSHDYDEIRGAVEGVTTFHFDDGPVTLEAGDILFIPAGVPHEVRTHNDRPFTAYKA
                     SISGERVVTEHGDGKGSVEDLQARS"
     gene            complement(1377..1574)
                     /locus_tag="PLG78_18955"
     CDS             complement(1377..1574)
                     /locus_tag="PLG78_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25261.1"
                     /translation="MENKEETSMESLVVASKAKLIIKNHDCMVAGDALDELNRKVHRL
                     IEDAVKRTKDNKRKTVQAYDF"
     gene            1692..>2112
                     /locus_tag="PLG78_18960"
     CDS             1692..>2112
                     /locus_tag="PLG78_18960"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE25262.1"
                     /translation="MRVVVTGGAGYIGSHTVLALLEAGHDVVVVDDLSTGHKINLPDG
                     VKFVPGRFQDDSVIQAALSESPDALFHFAALKAAGESMEEPEKYAAANIRGSLGLIER
                     CAASGVRNFVFSSSAAVYGEPQYLPIDENHPTVPINYY"
BASE COUNT          440 a          550 c          551 g          571 t
ORIGIN      
        1 taccagattc cagtttcacg cgtggcactg atgaccctta cgcggcttgc tccgcgcttg
       61 cgcgcatctt ttgtaaaact atcgagtgct gaatcgaggc ttgctctata gaccgggata
      121 agaccaaata gaaggtgcac gcctatgacg gttgtcttct gcatgatccc ggatggaccg
      181 tctactggaa ccatgccgtt gtattttgta gtgtcttctg tgaaggcaag ctgggagctt
      241 ctgcagctct gcgaagcgaa gcaggccacg attaccaggg agatccaggc gatttttttc
      301 atggagcgga cactggagtg tgccggtctt tctgcaattg aaaattccag atcaattccg
      361 aatcaattct agaacgtcgg atgggttttt tgccgtgaag tccggggaag gttcaggaac
      421 agaagagata taaccccacg aagcccagat gcttcttgcg ccagagtttc gcgccgcctg
      481 catgtctgcc gccgtgtctc cggtcatcca ccttgcacct gtttcaccca gtctgtccgc
      541 catagtttgc atgggaatcg gggatggttt gctttcgggg aaggaatctc ctccaatgat
      601 gcctgaaacg gtttccagga cctgcagtct tttcaagagt tcccttgaga ttctctccgg
      661 cttgtttgta tagaccacga gtcttccaat agacgctacg ccctgcaaca tagacgagat
      721 tccgtcatag agtctggtgt gatctacaac gtgcgacaga taatcctctt cgtagagggc
      781 ccgcactttt ttgaggtccg tttgattctc gatgatttcc gggaaggcag cctcagtaag
      841 ggcatccatc ccgcggttta ccagggagcg agccacttcg agcgagagtg cgggcagtga
      901 ttttgtttcg cgcactcgat tgatagacag agccatatcc tgtctcgagt cttcgagtgt
      961 gccgtccagg tcaaagaatg cgatcaactt cgtgcttgca ggtcttctac gctgcctttt
     1021 ccgtctccgt gttcggttac taccctttca cctgagatcg atgctttgta ggctgtgaag
     1081 ggacgatcgt tgtgcgtgcg cacttcgtgc gggactccag ctggaatgaa gaggatgtcg
     1141 cccgcttcca gtgttacggg cccatcgtca aaatgaaatg ttgtgacgcc ttccactgcg
     1201 ccccggattt catcgtagtc gtgtgaatgg cggcttcgct caaaccacgg cggaatggtc
     1261 tgaagatcgt aaacacgata tcctagaccg gccatttcct ttttcaccga ttcttcatcc
     1321 ggtgtttcct tatgtggcca ttttactact ctgactccgg ggggcattgg gaaacctcaa
     1381 aaatcgtatg cttgtactgt cttgcgcttg ttgtctttcg tgcgctttac tgcgtcctca
     1441 atgagacggt ggactttgcg attcagttca tcgagcgcat ctccagcaac catgcagtca
     1501 tggttcttga tgatgagctt tgccttgctc gcaacgacca ggctttccat tgaagtttcc
     1561 tctttgtttt ccataccgag agatttctcc tttcggcaag actgaaccgt cttttcgacg
     1621 catcaattca aaatcccacc cctcctggaa ttccgtttca cagggaaggg ggtccggagg
     1681 actctggtgc catgcgagtt gtcgttactg gcggggcagg ctacattggc tcccacaccg
     1741 ttctggccct tctagaggcc ggtcatgatg tagtcgtagt ggatgacctg tccacaggtc
     1801 acaaaatcaa cctgccggat ggagtgaaat tcgttccagg cagatttcag gacgactcag
     1861 tgatccaggc agccctttct gaatcgcctg acgcgctgtt ccattttgcg gctttgaaag
     1921 cagccgggga atccatggaa gagcctgaaa aatatgcagc agccaacatt cggggaagcc
     1981 ttggattgat agagagatgt gcggcgtccg gcgtacgcaa ttttgtattc agttccagtg
     2041 cggccgttta cggggagccg cagtacttgc ccatcgatga aaaccatccc accgttccaa
     2101 tcaattacta tg
//
LOCUS       DAOQJL010000630         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41918_length_2098_cov_4.479197, whole genome shotgun sequence.
ACCESSION   DAOQJL010000630 DAOQJL010000000
VERSION     DAOQJL010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41918_length_2098_cov_4.479197"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2098)
                     /locus_tag="PLG78_18965"
     CDS             complement(<1..>2098)
                     /locus_tag="PLG78_18965"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE25263.1"
                     /translation="SSSPPPIPAATTPHNVYDEPLIAPAPSFDDVPLPEFLDEPEVAA
                     SVEPDLRKPDAGSKPRGLLSRAEQARGEVSPSTEPAFEAELPVAPFDSAPAQADWSNE
                     ISPDFLNDLPAMDWDGDVAEGAPGQAAAGATEASIGEFPAEDSLALQSEDEASPESLP
                     GLWAELESEPSAEAETLQPEISAEHDDDFFREPAAENAGAVDEVLAEAEAAASESSGE
                     YESSEEDLEDLATEPEDPFEQYEAEARAEAEKQFGRDEDAPVAEETEAPSFLFDADNE
                     FTTAPIEAHIAGQKKIDHYLSLFDIAREISAIDDFEEFWDSILFSVMAQLGPETLTIF
                     ATRSTMEPGQKLFPVALSGYEGDDEWVLAPGQALYDACLREEGIIYAEQLRTVSEAIA
                     LSEMERDIIDRSKAQIIAPLKNGTNMYGIVLIGAAYSAEEYSVDDLEFVSLLGGIAGS
                     GVDRILARLEFDRDTEQLKRRDFFHNRILEAARECSGLRNLDELYDVLIRNLTEHFHV
                     DSYSLVLLNPATQEYSIFAGNRISPESIEKFRLRTTSELAGMVSNFTRVFEVNDFRAN
                     GEITACYTNDDLGIMKHYWVVPLIYQNWLVGFFTIHSTTQEWTEFDREMAVSFAELIG
                     PCFGGAIMLGERESLFRDPFSPLEDRLRLELKKSFEFHSHLSLAEIRIKNIKRILTLN
                     PSAQVTQFLSVVGKSIA"
BASE COUNT          506 a          554 c          531 g          507 t
ORIGIN      
        1 cagcgatgga tttacccaca acagacaaga actgagtcac ctgtgcggaa ggattcaaag
       61 taaggatgcg tttgatgttc ttgatgcgaa tttcagcaag actcaggtga gagtgaaact
      121 cgaatgattt ctttagttcc aggcgaaggc gatcttctag aggactgaat ggatcacgga
      181 acagagactc acgttcgcca agcatgatgg cgccgccaaa acaggggcca atcaattcag
      241 caaaagaaac agccatctca cgatcgaact ctgtccactc ctgtgtggtg gagtgaatcg
      301 tgaagaatcc aacgagccag ttctggtaga tcaagggaac cacccagtag tgcttcatga
      361 tccccagatc gtcgtttgtg tagcaggctg tgatctctcc gttggcgcgg aagtcattca
      421 cttcaaatac gcgagtgaag ttcgatacca tgcctgccag ttcacttgtg gtcctgagtc
      481 tgaacttctc aatggactca gggctgatgc gattgcccgc gaaaatggaa tactcctgcg
      541 ttgcaggatt gaggaggacg agcgaatagc tgtccacgtg gaagtgttcg gttagattgc
      601 gaatgagtac gtcatagagc tcatctagat tcctcaggcc tgaacattcc ctggcagctt
      661 ctagaattct attgtgaaag aaatctcttc tcttgagctg ctctgtatct cggtcaaatt
      721 ccaggcgcgc aagaattcta tccacacctg aaccggcaat cccgccaaga agagaaacaa
      781 actccagatc atccacggag tattcttccg cagaataggc agctccgatc aaaacgatcc
      841 cgtacatatt ggtaccattt ttcagaggtg cgatgatttg cgctttagag cgatcgatga
      901 tgtctctttc catctcgctg agcgcaattg cttccgatac agtgcgaagc tgctctgcat
      961 agatgattcc ttcttcgcgc agacatgcat catagagcgc ctgaccgggc gctagaaccc
     1021 attcatcatc tccttcgtat cccgaaagtg cgactggaaa taatttctga ccgggctcca
     1081 ttgtgcttcg cgttgcgaaa atcgtgagag tctccggccc cagctgtgcc atcacagaga
     1141 acaaaatgga atcccagaat tcttcgaaat catcaatcgc cgagatttcg cgagcgatat
     1201 caaacaaaga cagatagtga tcaatcttct tctggcccgc tatgtgagct tcaataggcg
     1261 cggttgtaaa ttcgttgtcc gcatcaaaga ggaaagacgg tgcttctgtt tcttccgcaa
     1321 cgggcgcatc ctcatcccgg ccaaactgtt tctccgcttc tgcgcgagct tctgcttcgt
     1381 attgttcaaa cggatcttct ggctcggttg cgagatcctc caggtcctcc tctgaggact
     1441 catactcccc tgatgattcg cttgcggccg cttccgcctc cgcaagtact tcatcgactg
     1501 cacccgcatt ttctgcggct ggttcgcgga agaaatcatc atcatgttcc gcggatatct
     1561 ccggttggag cgtttccgct tctgcggatg gttctgactc aagctcagcc cagagacctg
     1621 gaagtgattc aggtgatgct tcgtcttcgg actgtaaggc gagcgagtct tcggctggaa
     1681 attctccgat agatgcctcg gtcgctcccg ctgccgcctg tccgggggca ccttcagcta
     1741 catccccgtc ccagtccata gcaggcagat cattcaggaa atcaggagaa atctcattgg
     1801 accagtccgc ctgagcgggt gcagaatcaa acggagccac aggcaactct gcttcaaaag
     1861 caggctcggt ggacggagaa acttctccgc gtgcctgctc ggcacgtgat agaagtccac
     1921 gcggtttgga tccggcatcg ggcttcctca gatccggctc aacgcttgca gccacttccg
     1981 gttcatccag aaattccgga agcggaacat catcaaaaga aggagcaggc gcaatcaaag
     2041 gttcatcgta aacgttatgc ggcgttgtgg ctgcaggaat cggaggtggc gaagatga
//
LOCUS       DAOQJL010000631         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41920_length_2098_cov_3.584435, whole genome shotgun sequence.
ACCESSION   DAOQJL010000631 DAOQJL010000000
VERSION     DAOQJL010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41920_length_2098_cov_3.584435"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /gene="kdpB"
                     /locus_tag="PLG78_18970"
     CDS             <1..1389
                     /gene="kdpB"
                     /locus_tag="PLG78_18970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNE25264.1"
                     /translation="VFLVAVMSLPVFYEFAAGGKSSIPLPSLIALLVCLIPTTIAGLL
                     SAVRISGMNQLLAHNVLAMSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFFPAPGVE
                     EYDLASWAQLASLADETPEGRSIVVLAKNKFSLRERSLSDISAIAVPFSATTRMSGSD
                     LNMDGKQVAVRKGAFDAIAARVQSMNGSIPDAVKTRVDEIAKKGGTPLVVARDATVMG
                     CIYLKDIVKGGIRERFVELRKIGIRTVMITGDNALTAASIAAEAGVDDYIAEAKPETK
                     LARIRTEQSRGHMVAMIGDGTNDAPALAQADIGVAMQTGTQVAREAGNMVDLDSNPTK
                     LLEIVEIGKKILMTREALTTFSIANDVAKYFAILPAILGSLYAVAGVSPIASLNVMRL
                     SSPESAILSAVIFNALIIIAMIPIAMKGVQFRPMNASALLSRNLLIYGLGGLIAPFIG
                     IFALDIIVRRIV"
     gene            1386..1967
                     /gene="kdpC"
                     /locus_tag="PLG78_18975"
     CDS             1386..1967
                     /gene="kdpC"
                     /locus_tag="PLG78_18975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNE25265.1"
                     /translation="MRIIKHAFLSLASLIVLTVFTGWFYPWLVTTIARSAFPDRAEGS
                     LVRIGGEIRGSELIAQKFSGDRYFWPRPSAGDYATVPSSASNLGPSSAALAKAIQGRK
                     DSLAKAHNGIPPADMVTTSASGLDPEISPEAARYQLDRVARARRLNESQKEELRTLVE
                     QSVLEPTFGFLGESRVNVLRLNLALDERYPIQQ"
BASE COUNT          526 a          541 c          566 g          465 t
ORIGIN      
        1 gttttcctgg tagccgtgat gtctctgcct gtattctatg aattcgcagc aggtggcaag
       61 tccagcattc cactgccctc tctcatcgct cttcttgtct gcctgattcc aaccacaatc
      121 gcagggttgc tctccgcagt gcgaatcagt gggatgaatc aacttctagc gcataacgtc
      181 ctcgccatga gtggtcgtgc ggtagaagcg gcgggggacg tagacgtgct actgttggac
      241 aaaacgggaa ccatcacact gggcaatcgc caggcatccg agttttttcc agcaccaggc
      301 gtagaagagt acgatcttgc atcctgggcg caactcgcat ctctcgcgga tgaaacaccg
      361 gaaggcaggt cgattgtcgt tctcgccaag aataagttct ctctgcgcga acgatctctt
      421 tcggatattt ccgcaatcgc ggttcctttt tccgcaacga ctcgcatgag cggcagcgac
      481 ctgaatatgg atggcaagca agtcgctgtg cgcaaaggcg catttgatgc catcgccgca
      541 cgagtccaga gcatgaatgg aagcattccg gatgcggtaa aaactcgtgt agacgaaatc
      601 gcaaagaaag gaggcacacc tctagtggtg gcgcgcgatg ccactgtaat gggctgtatc
      661 tacttgaaag atattgtgaa gggaggaatc cgcgaacgat ttgttgagct gcgaaaaatt
      721 ggaattcgca ctgtgatgat cacgggggac aacgcgctga ctgcagcttc catcgctgca
      781 gaagcgggcg tagacgacta cattgcagaa gccaaaccgg aaacaaaact ggcgcggatc
      841 cgaacggaac aatccagagg gcatatggtt gccatgatcg gagatggaac gaatgacgca
      901 cctgcactcg cacaggcaga catcggagtg gccatgcaaa ccggaacgca ggtagcacgg
      961 gaagctggca atatggtaga tctggactcc aatccaacca agctccttga gatcgtagaa
     1021 attggaaaaa agatactcat gactcgtgag gcgctcacta cgttcagcat tgcaaacgat
     1081 gtcgcaaaat actttgcgat tctgcccgcg attttaggat cgctctacgc agtcgcagga
     1141 gtctccccga ttgcttcttt gaacgttatg cgactttctt cacctgagag tgcaatcctc
     1201 agtgcagtga tcttcaacgc cttgatcatc attgcaatga ttcctatcgc catgaaaggt
     1261 gtgcaattca ggccaatgaa tgcaagtgca cttttgtcca gaaacctgtt gatctacggg
     1321 ctcggcggtc tcatcgctcc tttcatagga atcttcgcac tggacatcat tgtaaggagg
     1381 attgtatgag aatcatcaaa catgcatttt taagtctggc ttctctgatc gtgctcacag
     1441 ttttcacggg atggttctat ccctggcttg taacaacaat cgccagatct gcgtttccgg
     1501 atcgagcgga aggcagcctg gtgcggattg gcggtgagat tcgcggatcc gaactgattg
     1561 ctcagaaatt ctcaggtgat cgctatttct ggcccaggcc atccgccgga gactacgcga
     1621 cagtaccatc ctctgcatcg aaccttggcc cttcgagcgc tgccctcgca aaggcaattc
     1681 aaggaagaaa agactcactc gcaaaggcgc ataacggcat tcctccggca gacatggtca
     1741 caacttccgc aagcggcctc gatcccgaaa tctcgcctga ggcagcgcgg tatcagctgg
     1801 acagagtagc aagagccaga cggctcaacg aaagccagaa agaagagctg cggaccctcg
     1861 tggagcaatc ggttcttgaa cccacatttg ggtttctcgg ggaatccagg gtgaatgtat
     1921 tgaggttgaa cctggcgctg gacgagcgat acccgattca gcaatgacag gcgaggactt
     1981 cagagcaacg cccgaagctc ttctcgagct cgcgcgaaag gaagagacga ccaaacaacg
     2041 cggcaagctg aagatctttt ttggcatggc cgccggagtg ggtaagacgt atgcaatg
//
LOCUS       DAOQJL010000632         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41921_length_2098_cov_3.473813, whole genome shotgun sequence.
ACCESSION   DAOQJL010000632 DAOQJL010000000
VERSION     DAOQJL010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41921_length_2098_cov_3.473813"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="ilvN"
                     /locus_tag="PLG78_18980"
     CDS             complement(<1..254)
                     /gene="ilvN"
                     /locus_tag="PLG78_18980"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE25266.1"
                     /translation="MKHILSILVKNRPGVMSHVSGLFTRRSYNIDSIAVGVTENPAVS
                     VITIVLKGDEKALGQFQGQLLKLPDVVEVKSLPFHDSLIR"
     gene            318..2063
                     /gene="sppA"
                     /locus_tag="PLG78_18985"
     CDS             318..2063
                     /gene="sppA"
                     /locus_tag="PLG78_18985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNE25267.1"
                     /translation="MIRILFQLLTLPLRFLWLLYCRIRNVRKPGSILVHRIPDRFTVF
                     RSGGVLSMFASRDDVHFAEYLALLGLMEEEREIQTVLISIPDVQHNMAETEQIAAALD
                     RIVKSGKKVIGHSEGGGIRTLYLLSCCSERFASPASSYSSFFPASEPHYFKGLLKKFG
                     VRMEVFSAGKYKSAGETFSRSGPSKEARENLEELISGLRANIARGMEARAPGFAKKFK
                     NKLLWSASELVTEGFFSREITEKDLARHLSGEPRETVKHAFSTVQPELEQKGPQLRIT
                     SDGALVRRHLRSRFKLLNLSRRRSFAFVAMEGAIYSGRKGDHARSGMISGHAYRDLLH
                     DLADSRDEAVIVWINSPGGTPDGSEIVYQALRYLGEKKPVVALMASVAASGGYYIASA
                     AHRIFSLESTITGSIGVVRLQPDAEKFYEKLGVESTRIGFEGSEDILSVSRPVGREGR
                     RLLDDQIQSTYSTFVQRVADGRGKSPAHVRRFAEGKVFTGKDFLASGLVDEIRGIPET
                     VDFLKSSMGIPAARRMQLSLYPEVRVDLRTLVSERFPISAALPLQALARQVSFPFALM
                     GKPVLYFPHEVVRFQ"
BASE COUNT          484 a          495 c          575 g          544 t
ORIGIN      
        1 tcccggatca gagagtcgtg aaatggaagg cttttcacct ctactacatc gggcaacttg
       61 agaagttgtc cctggaattg acccagagcc ttttcgtctc cttttagcac gatcgtgatt
      121 acagagacgg ctggattttc cgtgactcca acggcgatgc tgtcaatgtt gtagctccgg
      181 cgcgtgaaga ggcctgatac atggctcatc acaccgggcc ggttttttac aaggatggac
      241 aatatgtgct tcaagttgga ctcccatctc cccatcggac gggaagcttc tacgtcaagc
      301 gcaaatggcg gtgcagcatg attcggattc tcttccagct tctaactcta cctctgcgat
      361 tcctctggtt gctgtattgt agaattcgta atgttcgcaa accaggttcg attctggttc
      421 acaggatccc ggacagattc acagtatttc gcagcggtgg cgttctgtct atgttcgcat
      481 cacgcgatga tgtgcatttc gcggagtatc tggcattgct cggtcttatg gaagaagagc
      541 gcgaaatcca gactgttctg atttccattc cagacgttca gcacaatatg gcggaaacgg
      601 aacagattgc ggcggcactt gatcgcattg taaaatcagg aaagaaagta atcggtcaca
      661 gcgaaggagg tggaattcgc acgctttatt tgctctcctg ctgcagtgag cgattcgcct
      721 cgcctgcttc ttcttattca tctttcttcc ccgcaagcga acctcactac ttcaaagggt
      781 tgctcaagaa atttggtgtt cgcatggaag ttttttcagc gggcaaatac aagagcgccg
      841 gagaaacatt cagtcgttcc ggaccctcga aagaagcgcg cgagaatcta gaagaactca
      901 tcagcgggct gcgtgcgaac attgccagag gaatggaagc gagagccccg ggctttgcga
      961 agaaattcaa aaacaagctt ctctggtctg cttctgagct tgtgacggaa ggattctttt
     1021 ctcgcgagat cacggaaaag gatctggcgc gtcacctttc gggggagccc agggaaacag
     1081 tgaagcatgc tttctcaacc gtgcagccag aactcgaaca aaaaggacct caattgcgca
     1141 taacgtccga tggagctctg gttcgtcgtc atttgcgatc caggttcaag cttttgaacc
     1201 tgagtcgacg ccggagcttt gcttttgttg ctatggaagg agctatctat tcgggtcgaa
     1261 aaggggatca tgccagatca ggaatgattt ccgggcatgc gtatcgcgat ttgctccatg
     1321 atctggcaga ttcaagagac gaggcggtga tcgtctggat caacagtcca ggtggaacac
     1381 cggatggatc tgagatagta tatcaggctc ttcgttacct gggagaaaaa aagcccgtcg
     1441 tcgcactgat ggcatccgtg gctgcttccg gtggatacta catcgcctcc gccgcgcatc
     1501 gtattttttc tctggaatct acgatcacag gttcgattgg agtggttcgg ctgcaaccag
     1561 atgcagaaaa gttttatgag aagctcggag tagaatctac gcgaattgga tttgaaggga
     1621 gcgaagacat tctttcggtg tcgcgtcccg tgggtcgcga aggacgtcgc ttactggacg
     1681 atcaaattca atccacttat agtacgttcg tacagagagt ggcggatggc aggggaaaaa
     1741 gtccggctca tgtgaggcga tttgcagagg gaaaggtttt cactggaaaa gattttctgg
     1801 caagtggact ggtcgatgaa attcgcggca ttccggagac agttgatttt ctaaaatcgt
     1861 ccatggggat tcctgcagcc aggcgcatgc agctttcgct ttacccggaa gtcagagtgg
     1921 atctgcgcac tcttgtttcc gaaaggtttc ctatttctgc ggcccttcca ttgcaggcac
     1981 tggcaagaca ggtgagcttt ccttttgcac tgatgggcaa gccagttctg tacttcccgc
     2041 atgaggtggt tcgatttcag tgaggctgac gttctgtctc gccgtcgggc tgtgctgg
//
LOCUS       DAOQJL010000633         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_41944_length_2098_cov_2.510034, whole genome shotgun sequence.
ACCESSION   DAOQJL010000633 DAOQJL010000000
VERSION     DAOQJL010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41944_length_2098_cov_2.510034"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLG78_18990"
     CDS             complement(<1..999)
                     /locus_tag="PLG78_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNE25268.1"
                     /translation="MRIRNLCFTALALVWSAGLFAQEKFDPASVRSRDCGPPTYSCGF
                     TAPDPERARSIPVVPAVRMRGLPSSVDLSSQMPPVSNQGQQNSCVAWATTYATRSYFE
                     QKARQWGYDAPVKGGRGEHIFSPAYVYNQINGGRDQGSVMENAFDLLVRQGAAPWSVM
                     PYTDKDYTTQPTAAQRSAASNYKLASYRRIPPGDIETIKAELAAGRPVVFGMGIDDNF
                     YQLKNSVYDQKGGKTYGGHAMALVGYDDGKSSPRGHRGAFKIINSWGTGWGDKGYGWI
                     SYRQWTSEQPWALVGYPVDTPTQTQTTTTTTNPTPAPTPPQPVSEVTVPPPAQVNAT"
     gene            complement(1094..1612)
                     /locus_tag="PLG78_18995"
     CDS             complement(1094..1612)
                     /locus_tag="PLG78_18995"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNE25269.1"
                     /translation="MKVFRLVQFLIGCLIMALASCHGQKFEPVAAQTKLIDMNGDGQP
                     DRVSMDSQNRIWVEIAKEGGFQEPTIWWTPDRTVLPEFWLADVTGDGKPDLIVRAEGG
                     LRVAPGALNRFNPATEWLHELPPLSSEGIRFEDVDGDGKFDLILRGLDGEGNSYTSSG
                     QRFEPRHTKRKP"
     gene            complement(1699..>2098)
                     /locus_tag="PLG78_19000"
     CDS             complement(1699..>2098)
                     /locus_tag="PLG78_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25270.1"
                     /translation="VPLDKAFASSPIMTGGVVVGLLGVLAYASKKTWEQHATVYAMCD
                     HLDRLRWFRLYKNSIAAVQSVPWMQKGAGLFANLGLSVVSSASQSVVDGIVEHGIKKE
                     IAETAALIAVEYAVRIALVAGGLILTKIQA"
BASE COUNT          465 a          561 c          536 g          536 t
ORIGIN      
        1 ggttgcattg acctgagccg gtggaggtac agtcacttcg gacactggct gtggaggagt
       61 tggtgctgga gttggattcg ttgtagtagt tgtagtctgc gtttgagttg gtgtgtctac
      121 ggggtatcct actagagccc atggttgttc tgaagtccat tgcctgtagg atatccatcc
      181 gtatcctttg tcgccccagc ctgttcccca ggagttgatg attttgaatg cacctctgtg
      241 cccgcgcgga gaagatttac catcgtcata gccaacgagc gccatcgcat gcccgccgta
      301 tgtttttcct cccttctgat cgtagactga gtttttcagc tgatagaagt tgtcgtcgat
      361 tcccattcca aatacaacag gacgacccgc ggccagttct gctttgatcg tttcgatgtc
      421 tccgggtgga attcttctat aactggcgag tttgtaattc gatgccgcag aacgttgcgc
      481 tgcagttggc tgtgtggtat aatctttgtc cgtgtaaggc atgacgctcc atggggcggc
      541 accctggcga accagaagat cgaaagcatt ttccatcacg cttccctggt ctcttccacc
      601 gttgatttga ttgtaaacat acgctggtga aaagatgtgt tcccctcttc cgccttttac
      661 aggagcgtcg tatccccatt gtcttgcttt ttgttcgaag taggaacgcg tcgcatacgt
      721 cgtggcccag gcaacgcagc tattctgctg gccctgattg gaaacgggag gcatttgaga
      781 gcttaagtcc acagaagaag ggaggccgcg catgcgaacg gctggcacca cagggatgga
      841 ccttgctctt tctggatctg gcgcggtgaa tccacaggaa tacgtgggtg gaccgcaatc
      901 gcgcgagcga acggacgctg gatcaaattt ttcctgggca aacagccccg cagaccagac
      961 gagcgccaga gcagtgaagc aaagatttcg aattctcatg caatgtcctc ggttccgcag
     1021 cactccagtg cgcagacctc ttgatgtaga ctctctacac ccatcccgag ttcgtcacaa
     1081 gaacaaaatt gtcttaaggt ttgcgcttcg tgtgtcttgg ctcaaagcgc tgaccggatg
     1141 aggtatagct gtttccttcc ccgtccaggc cgcgaaggat caggtcaaat ttaccatcgc
     1201 catcgacatc ctcaaagcga atgccttccg atgaaagagg cggcagttcg tgcaaccatt
     1261 ctgtggccgg gttgaaacga ttgagcgccc ccggtgctac ccgtagtccg ccctctgcac
     1321 gcacgattaa atccggcttt ccgtctccag tcacatccgc cagccagaac tcggggagaa
     1381 ctgttctgtc cggagtccac cagatcgtcg gttcctggaa tccgccttcc tttgcaattt
     1441 ccacccatat gcgattttgt gaatccatcg aaacgcgatc tggttgccca tctccattca
     1501 tgtcgatgag tttggtttgt gctgcgacag gttcaaactt ctgaccatgg caggaagcga
     1561 gagccatgat aagacagcca atcagaaact ggacgagtct aaaaactttc ataccgttac
     1621 aattacgttc tcatagtttc agaggcaagc atcgaatttt ccccggagcc gccattcagg
     1681 ccaaaatgag gattcgtctc aagcctggat tttggttaaa attaagccac cggccaccag
     1741 ggcgatgcga accgcgtatt ctactgcaat gagcgccgcc gtttctgcga tttccttctt
     1801 gattccatgc tctacgattc cgtctacaac cgattgcgaa gcagaactta caacgctgag
     1861 tccaagattg gcaaacaatc cggctccctt ctgcatccag ggaacgctct gtactgccgc
     1921 gattgaattc ttatagagtc gaaaccatcg caggcgatcc aggtgatcgc acatagcgta
     1981 gacggttgca tgttgttccc aggttttttt ggatgcatag gcaagaactc caaggagtcc
     2041 aaccacaaca ccgcccgtca tgatgggcga agatgcaaat gctttgtcca gcggcacg
//
LOCUS       DAOQJL010000634         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42001_length_2096_cov_5.065164, whole genome shotgun sequence.
ACCESSION   DAOQJL010000634 DAOQJL010000000
VERSION     DAOQJL010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42001_length_2096_cov_5.065164"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLG78_19005"
     CDS             complement(<1..1067)
                     /locus_tag="PLG78_19005"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNE25271.1"
                     /translation="MGQRLPSRPLSLSGYCIGCFVAFMALVGCAQYNATPGVYGLALL
                     ANSGARCSAPTRASSLSATTTNTTVTAQFSTGACPATSSIVRRSTTGFPATVSSGTLV
                     SSTLTGFSDSGLTAGTVYYYSIFLSDSSGVTSSAANLSAIPGKTVVAPAQVANGSIVI
                     DGSDSDSAWSASPQFSFSYAEAYGESAGADNPSGYVRLAYDSQYFYFFIKSNDKYVHV
                     DGSVCCGQGQWTDDGVEVFFDMGFERATSPDANDFHLIFTPGSPAFNWYGKGPGAWGA
                     WTPTVNSGILINGTLNNETDTDTSWSMEVAIPLSDLGVSSISSGQSIGFTFWLNEDDT
                     VGHAAQHWYPYTTGTSSSNPST"
     gene            1184..1879
                     /locus_tag="PLG78_19010"
     CDS             1184..1879
                     /locus_tag="PLG78_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE25272.1"
                     /translation="MIEVKGLSRYYGDFLALDNVSIKIPPGEVVGLLGPNGAGKTTCL
                     RILTGFLFPSSGEVLIDGVDVFSNPLESKKKIGYLPEQPPLYPELLTFDYLQFVGRLR
                     GVEESSLKAEIERVAGLTEISDYLFSPIRTLSLGYRKRVGIAQALMGTPPFLIFDEPI
                     SGLDPRQIVEMRGLLRKIGKDHTVLISSHILTEVRHTCDRVFILHRGKLVREIAGPDL
                     DKIEDLFLQYTST"
     gene            1876..>2096
                     /locus_tag="PLG78_19015"
     CDS             1876..>2096
                     /locus_tag="PLG78_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25273.1"
                     /translation="MNQSNRLTFGLSRIKWIFIKEMKSFFGLNYPPVSLGIIALLCGI
                     VSALLPSTPGTTYEDVSRALFYLYYVFILV"
BASE COUNT          525 a          524 c          535 g          512 t
ORIGIN      
        1 gtactcggat ttgaagaaga tgttccggtc gtgtacggat accagtgctg tgctgcgtga
       61 cctaccgtgt catcttcatt caaccagaaa gtgaagccga tgctctggcc cgatgaaatg
      121 ctcgaaacgc ccaggtcact gagcgggatc gccacttcca tgctccagct tgtatctgta
      181 tctgtttcat tgttgagcgt accattgatg agaatgccag aatttacagt gggcgtccag
      241 gcaccccacg ctcccggtcc cttgccgtac cagttgaatg cgggcgagcc aggcgtgaag
      301 atgagatgaa agtcattcgc atccggggaa gtggctcttt caaaacccat gtcaaagaac
      361 acttctacac cgtcgtccgt ccattgtccc tgaccacagc agacgcttcc gtctacatga
      421 acatatttat cattagattt tataaaaaaa taaaagtatt gagaatcgta agccagacga
      481 acatatcctg acgggttgtc ggcgcctgca ctttcgccat acgcctccgc atacgaaaaa
      541 gaaaactgcg gagaagcact ccacgctgag tccgagtccg atccatcaat gacgatgctt
      601 ccgttggcca cttgcgccgg cgctacgact gtcttgccag gaatagcgga caggttcgct
      661 gcgctggacg tgactccaga tgagtccgaa agaaaaatgg aatagtagta cacagttcct
      721 gcagtcagcc cgctgtccga aaaaccagtc agagtagagg aaaccagcgt tccggaagac
      781 acggtagccg gaaaacccgt cgtcgacctg cggacaatgg aagaagtggc ggggcaagca
      841 ccggtgctaa attgcgctgt gactgtcgtg ttcgtcgttg tggcggaaag agaagacgct
      901 cttgtgggtg cgctgcacct ggctcccgaa ttcgcaagaa gtgcaagccc gtagacaccg
      961 ggggttgcat tgtactgcgc gcatccaacc aatgccatga atgccacgaa gcacccgatg
     1021 cagtagccgc tcagcgagag tggccgcgat ggcagacgtt gccccatgcg tgtaacgaac
     1081 gtgcaatgtt ccacccggac aagaataaat tcgtcaaaat cagcagagag acatcataga
     1141 aatccgttga ccgcaaactg gctattctgc gaccttaacg gcgatgattg aagtgaaagg
     1201 actcagccgt tattacggcg acttccttgc tctggacaat gtcagtatca aaatacctcc
     1261 aggtgaagta gttggattgc tcggcccaaa tggtgcaggc aaaactacgt gcttgagaat
     1321 cttgactggg tttttgtttc cgagctcggg agaagtgctc atcgacggtg tcgatgtgtt
     1381 ttcaaatcct ctggaaagca aaaagaagat tggctatttg ccagaacaac ccccgctcta
     1441 tcccgagctt ctaacgtttg attatttgca gtttgtggga aggttgcgcg gcgtagaaga
     1501 gtcttcgctg aaagcagaga tcgagcgagt tgcaggtctg actgaaattt cggactatct
     1561 gttctctcct attcgcactc tgtctctggg gtatcgcaaa agagtcggca ttgcgcaagc
     1621 tctgatgggt actccgccgt tcttgatttt tgatgaacct atttccggtc tggatccgcg
     1681 gcaaattgtg gaaatgcgcg gtttactccg caagatcggc aaagatcaca ctgttttgat
     1741 ttccagtcac attctgactg aagtcagaca cacctgtgat cgtgtattca tcctgcatcg
     1801 cggcaaactc gtgcgcgaaa tcgcaggtcc ggatctggac aagatcgaag atctattttt
     1861 gcagtataca tcaacatgaa tcaatcaaat cgcctaacgt ttggtctctc tcgaatcaag
     1921 tggatcttca tcaaagagat gaaatccttt tttggtttga attatccacc ggtatcgctc
     1981 ggaatcattg cactcttgtg cgggatagtg agcgcgcttt tgccaagtac accaggcacc
     2041 acctacgaag acgtaagtcg ggcgcttttt tatctctact atgtgttcat cctggt
//
LOCUS       DAOQJL010000635         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42050_length_2095_cov_3.699020, whole genome shotgun sequence.
ACCESSION   DAOQJL010000635 DAOQJL010000000
VERSION     DAOQJL010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42050_length_2095_cov_3.699020"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PLG78_19020"
     CDS             complement(<1..1395)
                     /locus_tag="PLG78_19020"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE25274.1"
                     /translation="MKRGGIVLLFVSLALIWGLVFTSVWKVTGFLLRLDVRRQQLLNY
                     ELSSRLLAEKVRSTEEDFDREVEGHVLETTVLNFASLGSSPGLWETGGIALVNGVRLV
                     SGKGILHVSDDESTLRLLQYAFALERTKKLKEAEAVFETIRPSLKDENLAFALLHGGY
                     CKFLLGEDDKARDSLTKVVEQYPGTHFAASARTLLALLNTKREDGESAADSAYRLGRY
                     AMAAREYSSMKSLTDRQQFKLARSLEETGKMPAAVELYRKLASGADPVRKDSIRRLLI
                     LGTFLGGGEPVRSFATEKAKENSDPALQKIEEARTLAEAPKFLGRKNVTPEGIVAELK
                     RENPAIRIPEEEAREEPPKVESKEPPAEKRAQIANPAPAAPTPIFRIPLIVRLSDGRA
                     ISCNQVRIHGSSARLIRQSATVETEVRSILSIESTDGYLTVNDSGIGTRRIDQEENFF
                     IVSGARRAYIPTSSL"
     gene            complement(1392..>2095)
                     /locus_tag="PLG78_19025"
     CDS             complement(1392..>2095)
                     /locus_tag="PLG78_19025"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNE25275.1"
                     /translation="IQNELTIGKEVQEQFLPDAKSLAKWNAAAISRPLREVSGDVYTF
                     FQAGGQDGVFFADASGHGVSAALITSLALLSLERIQETAYSAEEMALQMNAAIINHLK
                     TSMFYMTGVFALFDGDRIHYVNGGHPAPILIRKNGDIEILDPTGPPLGIKRDVSFEAG
                     SIEASSGDRLFLYSDGLIESPSPEGTVLDIEDVVNILMDHPDPTEAVNQIMVALDALL
                     PVVRDDISILYLGVP"
BASE COUNT          460 a          544 c          524 g          567 t
ORIGIN      
        1 cagcgaggaa gttggaatgt atgctcgccg cgcgccggaa acaatgaaga agttttcttc
       61 ttgatcaatt cttcttgtac caatccccga atcatttacc gttagatatc cgtcggtaga
      121 ttcaatgctg aggatggatc gtacttctgt ttccacagta gccgattgtc gaatcagcct
      181 ggcgctcgaa ccatggatgc gcacctgatt gcatgagatc gcgcgtccgt cgctcaatcg
      241 cacaatgaga ggaattctga aaatgggagt gggtgcggca ggtgccgggt ttgcgatttg
      301 tgctcgtttt tctgccggag gttctttgct ttccactttt ggcggttctt ctctcgcttc
      361 ttcctctgga attcgaatgg cagggttttc tcgcttgagc tccgctacaa ttccttccgg
      421 agtcacattc ttccgcccaa ggaatttggg agcttctgcc agtgtgcgag cttcttctat
      481 cttctgcaaa gcggggtcac tgttttcctt cgctttctct gtcgcaaagc tgcgaacggg
      541 ttctcctccg cccaggaatg ttcccagaat cagcagtctc cttatggaat cttttcgcac
      601 cggatctgcg cccgaagcaa gctttcgata gagttctacc gccgccggca tctttcccgt
      661 ctcttcgagg gaccgggcca gcttgaattg ctgcctgtct gtaagtgatt tcatggaact
      721 gtactctcga gcagccattg catagcgccc caggcgatac gcgctgtccg cagctgattc
      781 tccgtcttct ctctttgtat tgagaagggc gagtagagtt cgtgcagatg ccgcgaaatg
      841 agttccgggg tattgctcta ctacttttgt taaagaatct cgtgctttgt cgtcctctcc
      901 aagcagaaat ttgcagtaac caccgtgtaa caacgcaaag gcgagattct catctttgag
      961 ggaagggcgg atggtttcaa atactgcctc tgcttccttt agcttttttg ttctttcgag
     1021 cgcaaaagcg tactggagga gtcggagagt ggattcatca tctgacacat ggagaattcc
     1081 tttgcctgag acaaggcgta ctccgttgac cagtgcaatt ccgcccgtct cccatagacc
     1141 aggagagctt ccaaggctgg cgaaatttag caccgttgtt tccagcacat gcccttctac
     1201 ttcacgatca aagtcttcct cggtagagcg taccttttct gcgagcaagc gagaggaaag
     1261 ttcgtagttc agcaattgtt gtcgcctaac gtccaggcga agcagaaatc cagtcacttt
     1321 ccagacagaa gtaaaaacga gaccccagat caacgcgagc gaaacgaaca agagaacaat
     1381 cccgccgcgc ttcatggcac tcccagatac aggatgctaa tgtcgtctct tacgactgga
     1441 aggagcgcat cgagagctac catgatctga ttgactgctt ctgtcgggtc tggatgatcc
     1501 atgagaatgt ttaccacatc ttcgatgtcg agcacagtgc cttccgggga agggctttcg
     1561 atgagtccgt ctgagtaaag gaacagtcta tcaccactcg acgcctctat ggatccagct
     1621 tcaaaagata cgtctcgctt gattccgagg ggcggtccgg ttggatctag aatctcgatg
     1681 tccccgtttt tgcgaatcaa tatgggcgca ggatggcctc cgtttacata gtgaatgcga
     1741 tctccgtcga acagtgcgaa gacccccgtc atgtaaaaca tcgacgtttt taagtggttg
     1801 atgatggcag cgttcatttg cagtgccatt tcttccgcgg aataggcggt ttcctggatt
     1861 ctttcgagac tcaaaagcgc cagacttgtg atgagagccg cagatactcc atgcccggag
     1921 gcgtccgcaa aaaatacccc atcttgtcca cccgcctgaa agaacgtgta cacatctccg
     1981 ctgacttcac gaaggggcct ggaaatagcg gccgcattcc atttggcaag actctttgca
     2041 tccggaagaa actgttcctg cacttctttg ccaatggtga gttcgttttg tattt
//
LOCUS       DAOQJL010000636         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42085_length_2094_cov_4.260912, whole genome shotgun sequence.
ACCESSION   DAOQJL010000636 DAOQJL010000000
VERSION     DAOQJL010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42085_length_2094_cov_4.260912"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLG78_19030"
     CDS             <1..483
                     /locus_tag="PLG78_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE25276.1"
                     /translation="DVFIAPDAAVIGQVRIGAGSGVWFGSLVRGDVHSIDIGEHTNIQ
                     DHCVLHVTGGKFALRIGNHCTLGHRVTLHGCTLHDHAFMGIGSTVLDGCVIEEFGFLA
                     AGSLLPPGKTIPSRMLAMGSPAKVVREITAEEEEMMRRTAVKYSELARVYRSANFGPV
                     "
     gene            461..907
                     /locus_tag="PLG78_19035"
     CDS             461..907
                     /locus_tag="PLG78_19035"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNE25277.1"
                     /translation="MQTLDPSDIQKRSDRTELRIFQGGAVVVFLAGLFLPLTRFPSIC
                     IFLYITGLPCPGCGLTRSIHLTLNGKILQALQFHPFGLPALAWLCFLILSIFIPSLSR
                     FYVKHIKQIHRLYATGALLMLAFGVVRLIGLLMFGGLSWVAPSVLR"
     gene            949..1371
                     /gene="ndk"
                     /locus_tag="PLG78_19040"
     CDS             949..1371
                     /gene="ndk"
                     /locus_tag="PLG78_19040"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNE25278.1"
                     /translation="MEQTLIILKPDAVRNKHVGNIIARIEKEGFNIVALKKITLKDKD
                     AKKFYAVHKERPFYKDLCKFMTTGPVVVAVLEAPGAVEKWRKLIGATDPAQAEANTIR
                     KLYAESKEANAVHGSDSEKNAKGEIKFFFKAAELKGKL"
     gene            1372..>2094
                     /locus_tag="PLG78_19045"
     CDS             1372..>2094
                     /locus_tag="PLG78_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE25279.1"
                     /translation="MSDSEISLLREEFEQFLGEAGEIFSNGTVADLSAPVLYSLSAPG
                     KRLRPVLCMMFGGYQNKTQSRGLLFAAAALECIHTYSLIHDDLPAMDNDDLRRGIPTC
                     HKKFSEWAAILAGDALNTFAFELLAEAYEPVLLPEAVKTLARKGGRVGMIAGQALDLK
                     NERGSSSQESPSNANLQDTLQNIHLRKTAALIQASCVLGSLYAKTETESASGFGETLG
                     LLFQVSDDLLDARGSNDAGKATL"
BASE COUNT          529 a          530 c          541 g          494 t
ORIGIN      
        1 gatgttttca tcgctccgga cgcggccgtc attggccagg taagaattgg agcaggcagt
       61 ggcgtctggt ttgggtctct cgttcgcgga gacgtgcact ccatagacat aggggagcac
      121 acaaacattc aggatcactg cgtcctccac gtaaccggag gaaaatttgc tctgcgaatc
      181 ggaaatcatt gcactctggg tcatagagtc acccttcacg gttgcacttt gcatgatcat
      241 gcattcatgg gaatcggctc aacggttctt gacggttgcg tcattgagga gtttggtttt
      301 ttggcggcgg gaagcctgct tccacccggg aaaacaattc catctcgcat gcttgcgatg
      361 ggaagcccgg caaaggtagt gcgcgaaatt acagcggaag aagaagaaat gatgcgcaga
      421 actgctgtga aatacagcga actggcgcgg gtgtatcgca gtgcaaactt tggacccgtc
      481 tgacatccag aaacgaagtg atcgcacgga gctgcgcata tttcagggcg gggcagtggt
      541 cgtgtttctg gccggtctat tcctgccgct cacacgtttt ccttctatct gcatctttct
      601 ctacatcacc ggacttccct gtccggggtg tggtctaacg cgctcgattc atttgactct
      661 caacggcaag atcctgcagg cgctgcagtt tcatcccttc gggctcccgg cacttgcctg
      721 gctttgcttc ctgatactca gtatttttat accatctcta tcccgattct acgtaaaaca
      781 tattaagcag atccatagac tgtacgcgac aggtgctctc ttgatgctcg cattcggcgt
      841 agtacgactg atcgggcttt tgatgtttgg cggcctgagc tgggttgctc cttctgtctt
      901 gcgatagcag aagacttgac cccggcggtc caaaagcaaa gtacggttat ggaacaaaca
      961 ttgatcattt tgaagcccga tgcagtgcga aacaagcatg tcggaaacat catcgcacgc
     1021 attgagaagg aaggcttcaa tatagtagct ctcaagaaga ttaccctcaa agacaaagac
     1081 gcaaagaaat tttacgccgt tcacaaggaa cgcccgtttt acaaagatct ctgcaaattc
     1141 atgaccacag gtcctgtggt ggtagcggtt ctagaagcac ctggcgcggt agagaaatgg
     1201 cgcaaactca ttggagcaac agatccagcc caggcagaag caaacacgat tcgcaagctc
     1261 tatgcggaaa gcaaagaagc aaacgcagtt cacggatctg actccgaaaa gaatgcgaaa
     1321 ggagaaatca aattcttctt caaagcagca gagctcaagg gcaaactctg attgtcagac
     1381 agtgaaatct ctctcttaag agaggaattc gaacaattcc tcggagaagc cggggaaata
     1441 ttttcaaatg gtacggtcgc cgatctttcg gcgcccgtgc tctacagtct cagtgcaccg
     1501 ggcaaacgcc tcagacccgt actgtgcatg atgtttggag gctatcaaaa caagacacaa
     1561 tcacgtggcc ttctgtttgc ggccgcagca ctcgaatgca ttcatactta ctctctgatc
     1621 catgatgact tacctgccat ggacaatgac gatttgcgcc gtggaattcc aacctgtcac
     1681 aaaaaattca gcgaatgggc agcgatcctt gccggtgacg cgctcaatac gttcgccttt
     1741 gagctgttag ctgaagcata cgagcccgtt cttcttccag aggcagtgaa aactctggcg
     1801 cgaaagggtg gtcgagtcgg gatgattgcc ggacaggcac tcgatttgaa aaatgagcga
     1861 ggttccagtt cgcaagaatc gccatcgaac gcaaacctgc aggatactct gcaaaacatt
     1921 catctgcgaa aaacggccgc actcatccag gcgtcgtgcg tccttggctc tctgtatgca
     1981 aaaactgaaa cggaatctgc gtccggattc ggggaaacgc tcggcttgct cttccaggta
     2041 tcagacgatc tcctggatgc gcgcggttcg aatgatgcgg gcaaagcaac tttg
//
LOCUS       DAOQJL010000637         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42342_length_2088_cov_4.657649, whole genome shotgun sequence.
ACCESSION   DAOQJL010000637 DAOQJL010000000
VERSION     DAOQJL010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42342_length_2088_cov_4.657649"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PLG78_19050"
     CDS             complement(<1..2026)
                     /locus_tag="PLG78_19050"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNE25280.1"
                     /translation="MAHRGRLNVLTNVMGKDPAMIFAEFNEKVTNDVGPGDVKYHLGY
                     SSDAVTSTGKKIHLSLGFNPSHLEVINPVVLGSVRARQTNGKDIERTQNLPLVIHGDA
                     AFAGQGINYECLQMSSLEGYTVGGAFHIICNNQVGFTTNPADARSTRYCTDLAKMLQV
                     PIFHVNGDDPEACYRAVQLCMEWRQRYGTDVFLDIVCYRRWGHNETDEPTFTQPVMYD
                     AVKNHPTTVTLYEQRLLQEGIPAEEVEAIKKKSRDHFEEAYQRFQGGGVEVQFETLKG
                     KWTGFTRINPSEPVTAVAEDELRAVADILTRAPGGFSVHPKIQKLLEGRRAMLAPGGK
                     IDWGMGEALAYGTLLRQGYPIRISGQDVKRGTFSHRHAVLFDVKTDEEYAPLAQTAQK
                     GAHLQIYNSLLSEVAVLGFEFGYSLADPHSLVIWEAQFGDFANGAQVIIDQFICSCEA
                     KWSRMSGLVMLLPHGYEGQGPEHSSARLERYLQLCSLNNIIVANCTTPAQFFHIIRRQ
                     MLRNYRKPLIIMSPKSLLRLPEATSSLSELTSQAFDPVIDETDPSIDPAKVKRVVFCT
                     GKLYYELSAERRKQGVNHVALVRVEELYPYPVKEISSVIQRYPNAREFAWAQEEPRNQ
                     GAWTYIDPRLRSQIPQASELSYIGRTPSPSPATGYFKVHQKEQQEIMDQVL"
BASE COUNT          461 a          521 c          526 g          580 t
ORIGIN      
        1 cgaggacctg gtccatgatt tcttgctgtt ctttttgatg cactttgaag tagccagtgg
       61 ccgggcttgg cgaaggcgta cggccaatgt aagatagttc actcgcttgc ggaatctgag
      121 agcgcaaacg cggatcaatg taggtccacg cgccctgatt gcgcggctct tcctgggccc
      181 aggcaaattc acgcgcattt ggatatcgct gaatgacgct tgagatttct tttacggggt
      241 agggatacag ttcctctact cgcacaagtg cgacgtggtt cactccctgc ttcctgcgtt
      301 ctgctgacag ctcatagtaa agtttgcctg tgcagaacac aactctcttg actttcgccg
      361 gatctatgga tggatccgtt tcgtctatga ctggatcaaa cgcctgcgat gtgagttccg
      421 ataaactgct ggttgcttcc ggcaatctca aaagagattt cgggctcatg atgatcagag
      481 gcttacgata gtttcgtagc atctgacggc ggatgatgtg gaagaactgc gcgggcgttg
      541 tgcaattcgc aacaatgatg ttgttcagtg aacacaattg caaataacgt tcgagtcttg
      601 cgcttgaatg ctctggaccc tggccttcat agccatgcgg aagaagcatc acaagcccgc
      661 tcattctgga ccattttgct tcgcatgaac agatgaactg atcgatgatc acctgcgcgc
      721 cgtttgcaaa atcaccaaac tgcgcctccc agatgacaag gctgtgtgga tctgcgagag
      781 aatagccaaa ctcaaatcca agaactgcga cctcagaaag cagactgttg tagatctgaa
      841 gatgggcgcc tttttgtgct gtctgcgcaa gcggtgcata ttcttcatct gttttgacgt
      901 cgaatagaac tgcatgtctg tggctgaaag ttccgcgctt tacatcctgg cccgaaatac
      961 gaattggata tccctggcga aggagcgtgc cgtaggcgag tgcttcaccc atgccccagt
     1021 caatctttcc acctggcgcg agcatcgcgc gacgaccttc gagtagcttc tgaattttag
     1081 gatgaacaga aaatccgcct ggtgcgcgag tgagaatatc tgccactgca cgaagttcgt
     1141 cttctgctac tgcagtcaca ggttctgatg gattgatgcg agtgaatcca gtccatttgc
     1201 ctttcaaagt ttcaaattga acttcaacac cgcctccttg aaaacgctgg tatgcttctt
     1261 caaagtggtc gcggctcttc ttcttgatgg cctctacttc ttctgcaggg attccttcct
     1321 ggaggaggcg ctgttcgtat aacgttactg tggttggatg attctttaca gcatcataca
     1381 tcaccggctg ggtgaaggtt ggttcgtctg tttcattgtg gccccatctg cggtagcaca
     1441 ctatgtcgag aaacacgtcc gttccgtatc tctgtctcca ttccatacac agctgcaccg
     1501 cacgatagca tgcttccgga tcatccccat tgacatggaa gattggcacc tgaagcattt
     1561 tcgcgaggtc cgtgcagtaa cgagtggatc ttgcatctgc gggatttgtt gtaaatccaa
     1621 cctgattgtt gcagatgatg tggaaagctc cgcccacagt gtacccttcc aggcttgaca
     1681 tttgcaggca ttcgtagttg attccctgtc cggcgaatgc tgcatctcca tgaatcacca
     1741 gaggcaaatt ctgagtgcgt tctatatctt ttccgtttgt ttgtcgtgcg cgcacagatc
     1801 ccaggacaac tggattgatg acttcgaggt ggctgggatt aaacccgaga ctgaggtgga
     1861 ttttttttcc tgtagatgtg accgcgtcgc tggagtagcc cagatggtat tttacatccc
     1921 ctggtcctac gtcgttggtt actttttcgt tgaactccgc gaagatcatg gctggatctt
     1981 ttcccatcac attggtcaga acgttcaatc gtcctcggtg agccatgcct atgatgattg
     2041 atactacgct gaatccgcca gaaatttcca caagagcaga taacgttg
//
LOCUS       DAOQJL010000638         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42475_length_2085_cov_3.759113, whole genome shotgun sequence.
ACCESSION   DAOQJL010000638 DAOQJL010000000
VERSION     DAOQJL010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42475_length_2085_cov_3.759113"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1394..1846)
                     /locus_tag="PLG78_19055"
     CDS             complement(1394..1846)
                     /locus_tag="PLG78_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25281.1"
                     /translation="MQKRTSYAISVLLAFWMPAQILSERPKQSTEEVVKEALAAIEKG
                     DGELIEGMRDKIKTQHLAPIAATWKASFPWSKKDWYIALLMDHQDPILTPMMEDALDS
                     PTAESRAYALMILKKDFSLHKDLWTKSGWIIPAKVDEAVKKYRAEKKK"
     gene            1884..>2085
                     /locus_tag="PLG78_19060"
     CDS             1884..>2085
                     /locus_tag="PLG78_19060"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25282.1"
                     /translation="MRPTGTVIRSFFNWSGGKDSALALYHCKQNPEVDIAHLVTSVNA
                     HHGRISMHGVRESLLDAQAEALG"
BASE COUNT          463 a          522 c          570 g          530 t
ORIGIN      
        1 gttttggaga aacagccctc ttcttcgcgc ggagcaacgc aaatgttacg cgattgctga
       61 tcgcggcgaa agcggacctc aatgcgcgtg atcaatgggg ccataccgcc ttgtactggg
      121 ccaaaaagcg ctcttgcgaa atagacctat tcggcctttg tctctatcac ggtgccgatg
      181 aggttgcgga tttgctcaga gcctccgggg cagaggactg acctatgcgt taccttagag
      241 tatacagcct ttggactttc ttggtcttgg ttgtgagctg cgattgcagg aagtccccgg
      301 aactgatgcc cgcccaggaa ctttatcggg cttgcacagg tcagggaata agctatacaa
      361 ctcttcagcc agaacgcggg cttgtgagat cgaaacatgt agaggattac tttaaatgca
      421 aggatcctgc agagagcgcg cgctgcccta ttcgcgcggc cggtgaggag gagaacaatg
      481 atcgggcgta ccgccaggca tttgactacg tgaggacagc ctatatttgc attcaccttc
      541 ccagtggcca aacgtccacg tacggtggtg ggcgggcttc cgtcactgtg aacgctgccc
      601 accagcaggt gctccgtatt ccacagctct tgttcctcac aagagatcaa gagtggaact
      661 ggatcgcacg cttgaaattc gcatcccttc gaatgccacg ctcgctgatc tggacatcta
      721 cattttcgcg aagccgacgc ttccaaagaa cagtgcatcg tcactcgacg tcaagctttc
      781 ggatctaacg gcctatgcag tggtcaaaca cgattgcgtt ttcgaagctg aacaaaggca
      841 cagtggaccc ttccaattat cctacgacgc ggctagcagg tgcgcgaatc agttactgtt
      901 gtcggtgccg actggaaaaa gcgcagattt gaccacagaa ggactggaga atctcctgga
      961 ggacaactct ccatcacgga agtagccgcg gactcagagg aacggattcg cgattgcggt
     1021 ttggcacgta acccaagccg aatgaggacg tgaaagaatg ggctcgaagt atttcagatt
     1081 cgctttgcgg aaggtttcat aacgtgagac gcacagcggg atttgcttaa cctggatcac
     1141 ttcatggctt ctggtttctt tgctttgcac atgcgtggat cgcgggggaa caactgccgc
     1201 tgctgcattt gaatgcctgg atcgcgagaa atctgtcttc aaggtgaatc tcttgaactg
     1261 ttagaggcat cgaggagatt tgagcggggg tttgagagat ttcagaaacg cagcggcact
     1321 ttgcgcggtt tttttagttt tcaattgagc tttcgccatc ttttcgatct tgccttcact
     1381 gcacgcgaag gggttatttc tttttttctg cccggtattt cttcacagct tcgtcgacct
     1441 tcgcgggaat aatccacccg ctctttgtcc agagatcctt gtggaggctg aagtcctttt
     1501 tgagaatcat cagcgcgtat gcgcggcttt ctgccgtcgg agaatcgagc gcgtcttcca
     1561 tcattggcgt gagaatggga tcctgatggt ccattagcag ggcgatgtac cagtcttttt
     1621 tcgaccaggg gaaagatgcc ttccaggtgg ctgcaatggg cgcaagatgc tgcgttttga
     1681 ttttgtcccg cattccttca atcaattctc catcaccttt ctcgatggct gcgagggctt
     1741 ctttgactac ttcctctgta ctttgctttg gtcgttccga tagtatctgc gcgggcatcc
     1801 agaaagcgag cagaacagaa atcgcataag aagttctttt ttgcattcgc cagaaagtgg
     1861 aagtttgttc cgactgtcaa gctatgagac ctaccgggac tgtgattcga tcctttttca
     1921 actggagcgg aggcaaagac agcgctctgg ctctctatca ctgcaaacaa aatccggagg
     1981 tcgacatcgc gcatctggtg acctctgtga acgcgcatca tggtcgaatc tccatgcacg
     2041 gagtgaggga gagcttgctg gacgcgcagg cagaggcgct cggga
//
LOCUS       DAOQJL010000639         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_42802_length_2077_cov_5.501978, whole genome shotgun sequence.
ACCESSION   DAOQJL010000639 DAOQJL010000000
VERSION     DAOQJL010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42802_length_2077_cov_5.501978"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLG78_19065"
     CDS             complement(<1..1530)
                     /locus_tag="PLG78_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE25283.1"
                     /translation="MSELLITKHIPDMYARAARLFAQRPAFATRVKGTTDWQSITFQE
                     LYETGLDLATALIDLGVKARDHVALLADNRLEWIIADYGIQLCGAADVPRGTDVTDMD
                     IQYILPHSEAKVVFVEHLEMLKKLDKNKRKLSKIKHIILMEKSAKAPKNVLSMYDLID
                     KGRAMRARGNKKAEERIAGIKPDDLFTLIYTSGTTGAPKGVMLTHANICSQFKHEIVP
                     LNDHDRILSILPVWHIFERGFEMLAISRGCCTYYTNVRSLGEDIKLVKPTFMGSAPRL
                     WESIYLKIMDGVKSASPVKRGMFRAAYFCSRMFQGSLFFITGKKLDMEGRNPAVSAIT
                     GIGHALRAVVFAVPYFLLDMIVLKKMRTATGGHIKGTVSGGGALPLHVDEFFNYIGIP
                     VLEGYGMTETAPVLAVRNFEKRVIGTVGPVFPQTEIRIIDLNSGDVLFPNPKNRADGR
                     GLKGEIHAKGPQVMAGYFKNPEATKRVLRDGWMNTGDIGMITFNDCVKIMGRSKDTVV
                     LI"
     gene            complement(1600..2052)
                     /locus_tag="PLG78_19070"
     CDS             complement(1600..2052)
                     /locus_tag="PLG78_19070"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNE25284.1"
                     /translation="MKKKSLIILAVVLALPFALAGGLAVFSVGAYYVASCAASKQYKD
                     ASRPGHISNLAVFEFADEFRVNLADGRPLRAKIALGFEKGSPAEGQINERKDVLRNIA
                     NLVLSGKRKADITSVQQQLDLREELRASFNHVVGGGIEEVYFNEFTLD"
BASE COUNT          472 a          539 c          495 g          571 t
ORIGIN      
        1 gatcagcaca actgtgtctt tggatcgacc cataatcttg acacaatcgt tgaacgtgat
       61 cattccaata tcacctgtgt tcatccatcc atcgcgcaga actcgtttgg ttgcctcagg
      121 attcttgaaa tagcctgcca tgacctgcgg gcccttcgcg tgaatttctc ctttgagtcc
      181 acggccatcc gcgcgattct ttggatttgg aaacagcacg tctcccgagt tcaagtcaat
      241 gatgcgaatt tctgtttgag ggaacacagg accaaccgtt ccaatgactc ttttttcgaa
      301 attgcgcacc gccagtacgg gagctgtttc cgtcatccca taaccttcga gtactggaat
      361 tccaatatag ttgaagaatt catccacatg caatggaaga gcaccaccac cggatacagt
      421 tcctttgatg tggcctcctg tggccgttct catctttttt agtacgatca tatccagcaa
      481 aaagtatggt accgcaaaga caactgctcg cagtgcgtga ccaattcctg tgatcgcaga
      541 tacggcaggg tttctacctt ccatatccag tttcttgcca gtgatgaaaa ataaagatcc
      601 ctggaacatg cgcgaacaga aataagccgc tctaaacatt cctcttttta cagggcttgc
      661 ggattttacc ccgtccatga tcttcaaata gatgctttcc cagagtctgg gcgcagaacc
      721 catgaaggtt ggtttcacaa gcttgatgtc ctctcccagg ctgcgaacgt ttgtatagta
      781 cgtgcagcag ccgcgagaga tggcgagcat ctcgaatcct cgttcgaaga tgtgccatac
      841 gggaagaatg gagagaattc gatcatgatc gtttaacggc acgatttcat gtttgaactg
      901 agagcaaatg ttcgcgtgag tcagcatcac tcccttgggt gcccctgtag ttcccgaagt
      961 gtagatcaaa gtgaaaagat cgtctggttt gatacctgcg attctttctt ccgctttctt
     1021 gttccctctg gctcgcatgg cgcgcccttt gtcgattaga tcgtacatgc tcagtacatt
     1081 ctttggtgcc ttcgcactct tttccatgag gatgatgtgt ttgattttgg agagcttacg
     1141 cttgttcttg tccagtttct tgagcatttc aagatgttcc acaaatacaa ccttcgcctc
     1201 tgagtgcgga aggatgtact ggatatccat gtctgtgaca tctgtgccgc gaggaacgtc
     1261 tgctgccccg caaagttgaa ttccgtagtc cgcgatgatc cattcgagtc tattgtccgc
     1321 caggagggca acgtgatctc gggcttttac gccaaggtca atgagtgccg tagccagatc
     1381 caggccggtt tcgtataact cctgaaaggt gatgctctgc cagtccgtag ttcctttgac
     1441 gcgcgttgcg aaagctggtc tttgcgcaaa gagccttgct gctcttgcgt acatgtctgg
     1501 tatatgtttt gtgatgagaa gttcggacat tgatggttgc ctcaggtgcg taaaggacgt
     1561 ccgggggcaa agactgcaat caaaaacgca gtcgaatcat caatccaggg taaattcgtt
     1621 aaagtatact tcctcgattc cgcctcctac aacgtggttg aagctcgctc tgagttcctc
     1681 ccttagatcg agttgctgct ggacgctggt tatgtctgcc ttgcgctttc cggacagaac
     1741 cagattggcg atgttgcgaa gaacgtcttt gcgctcgttg atttgccctt ccgccggact
     1801 ccctttttca aatccaagcg ctatcttcgc tctcagggga cgaccatctg ccagattgac
     1861 ccggaattca tctgcgaatt caaagaccgc aagattcgaa atgtgccccg gccggctcgc
     1921 atccttgtat tgcttgcttg ccgcacagga tgctacgtag tacgcaccaa cggaaaagac
     1981 cgccagccct ccggccagag cgaaaggcaa agccaggaca acggcaagaa taatcagtga
     2041 cttctttttc accggcgtgc acccgcgtta gcttttc
//
LOCUS       DAOQJL010000640         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43264_length_2066_cov_3.884137, whole genome shotgun sequence.
ACCESSION   DAOQJL010000640 DAOQJL010000000
VERSION     DAOQJL010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43264_length_2066_cov_3.884137"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLG78_19075"
     CDS             complement(<1..259)
                     /locus_tag="PLG78_19075"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25285.1"
                     /translation="MAFVLHGRMTAWVAVDTMSGDHGPEPAVHGAQQAIREYGSHVTL
                     VGDPEKLEKLLKRFTCDPSHVQIAPAKEVITMDDSPARAVRS"
     gene            317..1057
                     /locus_tag="PLG78_19080"
     CDS             317..1057
                     /locus_tag="PLG78_19080"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNE25286.1"
                     /translation="MRAIVFFLCLTTCTTAGVMLPVNDVACKTGGRRVPDGAIPTPED
                     LAKDAFCAQRVIDRAAPHGVVTIFGSARAKEDWDSYKITRKFAYLWSKENSRYPILTG
                     GGPGLMEAGNRGAKEAGAPSLYFSTYFGSGSEKPNSYTTDGFMFASFAQREADMVDRA
                     AAVVIGQGGVGTEWEIFETLSKVQTRKKARCPIVLLGKRSDWASLFARMDAMKQSRTV
                     SPGDSDLMVVAETPEEAVAIIRAGIRDL"
     gene            1054..>2066
                     /locus_tag="PLG78_19085"
     CDS             1054..>2066
                     /locus_tag="PLG78_19085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE25287.1"
                     /translation="MIEALKAFIRPAPEMPRIQDPTRIKSDYRYWRTRIMYSMMSGYA
                     VYYFVRKNFSIANKSISEEFHFSNTEMGLILSAATVAYGISKFASGILVDILNPRFLM
                     SAGLILSALTNIFFGMGAGLTVFIVLWALNNLFQGLGMPPCGKLLNSWFSRKEGGRAW
                     GLWNASHQIGGGLIFLIAGFLVSEYGWRSSFYVPGVFAILVGLFLINRLRDTPAALGL
                     PPVHEYAGESDPFKEAQTQSGREIFINYILKNRILWIVCIANIFVYIVRIGVFDWAPK
                     FLVSERGFTTKEASVAGFAFEVSGIFGAIAAGWLSDTAFPGRRGAIATVFMVWLAAAI
                     GIV"
BASE COUNT          482 a          496 c          551 g          537 t
ORIGIN      
        1 tggatctcac cgctcgagcc gggctatcgt ccattgtgat tacttctttg gcgggcgcga
       61 tttgaacgtg cgatggatcg cacgtgaagc gtttgagcag tttctctaat ttttcaggat
      121 cacctacgag agttacgtga gagccgtatt cgcggatggc ctgttgcgcg ccatgaaccg
      181 caggctccgg accgtgatct ccgctcattg tgtctacagc tacccaggca gtcatgcgcc
      241 cgtgtaacac aaaagccatg aatcgtaaag aatttaactg ttgcaaaaca aatgatgagg
      301 accaaacaag tgccccgtga gagccatcgt attcttcctc tgtttaacga cctgcaccac
      361 agccggcgtc atgcttccgg tcaacgacgt cgcttgcaag acgggcggta gacgcgttcc
      421 ggacggagcc atacccactc cagaagacct ggccaaagac gccttttgcg cgcagagggt
      481 gatcgatcgc gcggcccctc acggagtcgt taccatattc ggatctgccc gcgcgaaaga
      541 ggactgggac tcttacaaga tcacgagaaa gtttgcttat ctgtggtcca aagaaaatag
      601 ccgttatccg atcttgacag gaggcggacc cggtctcatg gaagcaggaa atcgcggcgc
      661 gaaagaggcg ggagcgccca gtttgtactt ctcaacttat tttggatcgg gctctgagaa
      721 gccaaactcg tacactacag atggttttat gtttgcaagc ttcgctcagc gtgaggcgga
      781 catggtagac agagcggcag cggtggtcat cgggcaggga ggcgtgggca cagagtggga
      841 gatttttgag acactctcca aggtgcaaac tcgcaagaaa gctcgttgtc ccatcgttct
      901 gctcggaaaa agatccgatt gggccagtct tttcgctcgt atggatgcca tgaagcagtc
      961 tcgcacagtc tctccaggtg attcagatct catggtggtg gcagaaacac cagaagaagc
     1021 agtggccatc attcgtgcgg ggattcggga tctatgatag aagctctaaa ggcattcatc
     1081 cgtcctgctc ctgaaatgcc tcgcattcag gaccccacac gaatcaagag cgactatcgt
     1141 tattggcgaa cacgaatcat gtatagcatg atgtccggct atgctgttta ctattttgta
     1201 agaaagaatt tctccattgc aaataaatcc atttccgaag agtttcactt ctcaaataca
     1261 gagatgggac tcatcctcag tgcagccaca gtcgcctatg gaatttcaaa attcgcaagt
     1321 ggcattctgg tagatatcct caatccacga tttctcatgt ctgcaggctt gatcctttcc
     1381 gctttgacga atattttctt tggaatggga gccggactga ctgtattcat tgtactgtgg
     1441 gcactcaaca atctatttca agggctgggc atgcctccct gtggaaagct tttgaattcc
     1501 tggtttagca ggaaggaagg cgggcgagcg tggggtctat ggaatgcgtc gcatcagatc
     1561 ggcggtggtc tgatctttct cattgctgga tttctcgtca gcgaatatgg ttggagatcc
     1621 tctttctatg ttccaggagt ctttgctatt ctggttggac tctttttgat caatcgcctg
     1681 cgcgatactc cagcagctct gggtcttcca ccggttcatg aatacgcagg agaatcagat
     1741 ccattcaaag aagcacagac acagagcggc cgcgaaatat tcataaacta tatattaaaa
     1801 aatcgtatat tatggattgt atgcatcgct aatatattcg tctatattgt gcgaatcggc
     1861 gtctttgact gggcacccaa gtttctggtg agtgaacgcg gttttactac gaaagaggcc
     1921 agcgtggcgg gctttgcttt tgaggtttct gggatctttg gagcgatcgc ggctggctgg
     1981 ttgtcagaca ctgcctttcc gggccgcaga ggtgcgatcg ccactgtatt tatggtgtgg
     2041 cttgcggccg ccataggaat cgtttt
//
LOCUS       DAOQJL010000641         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43296_length_2065_cov_3.778607, whole genome shotgun sequence.
ACCESSION   DAOQJL010000641 DAOQJL010000000
VERSION     DAOQJL010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43296_length_2065_cov_3.778607"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..>2065
                     /locus_tag="PLG78_19090"
     CDS             288..>2065
                     /locus_tag="PLG78_19090"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HNE25288.1"
                     /translation="MNLRWFREHGISVVEFLRASHVLSGLDRPALKALARSFVPITLQ
                     PGESVMKQGEPGDSLFLVASGRLRATRVRADGSETTLGEICTGEIVGEAAVLTDEPRY
                     ANVSVVEQADLLRLSRTDFNSLLATHPAEIRKLSHIIADRQEQGHTERFRPVSRNLIE
                     FLKNVPLFAFLPGPLLKEIEPHLTWLHLPAGRVLMRQGEEADGLYVVVGGRLRFESVD
                     ERGVKRSGDFGRGEIIGELALLTGDSRSATVRAVRDSELVKLSDVSVQRMLHEAPHAL
                     FWLTRILAERLTRDQAEPVRRFSVLTVLPVSSGVDMNAFCTGLKESLSFHGNVELMTP
                     QRVDEKFGPGTASLEMEDPRASEFMIWLSDIEHAVDYLLLQGSTDMSWNERCIRQADK
                     ILLVADAGQDPRLAPVELKYPPDAEHVSGERNLVLIQPADALRAHNTKDFLANRGNMR
                     HFHVRRNSSADFQRLARGLTGNSIGLVLGGGGARGMAHIGVLKALIEHQVPIDMVGGT
                     SAGAIMAGTFAFFQDLERVSITVREFMVDRNPLNDYTFPFLSLARGRKYSEALERVFG
                     NVSLEDLLLPAFAIACNLSTSQEACL"
BASE COUNT          429 a          555 c          568 g          513 t
ORIGIN      
        1 ctttgatgac tggcattacc cgtgtcagac acatttcgtc aattgccgca tgcgaagcgc
       61 actattcttt tccatccacc agaccaccag agggccgcag aatgcccatc cggaacttca
      121 cgagcgaacg atagacggct ttgtgatccc ttgcattgcg aaaaagcgag agctgaatgc
      181 gatcagactt ctttcagctt cggaagtttt tctcaaatct tttcccgttc tcctcgctcc
      241 cggtggatgt cgcttgacat gcttgttcag tggtctcaag gcgtactatg aatcttcgct
      301 ggtttcgtga gcatggaatt tctgtcgtcg agttcctcag agcatcgcat gttctttctg
      361 gtcttgaccg cccggctttg aaggcacttg cccgctcttt tgttccaatc acactccaac
      421 caggcgaatc tgtcatgaag cagggggagc cgggggattc tctatttctc gtcgcctccg
      481 gtcggttgcg cgcaacgcga gtacgtgcag acgggagtga gaccaccctg ggcgagattt
      541 gcacaggcga aattgtaggg gaagctgctg tcctcacgga tgaacctcga tacgcgaatg
      601 tctccgtcgt ggaacaggca gatctgctgc gcctgtctcg aaccgatttc aacagccttc
      661 ttgcaactca tcccgcagag atccgcaaac tctcgcacat catcgccgac cgtcaggagc
      721 aaggccatac agaaaggttt cgtccggttt cgcgcaatct gattgaattt cttaagaatg
      781 ttccgctctt tgcgtttctg cctggtccct tgctgaagga gatcgagcct catctgacgt
      841 ggctgcatct tcctgctggc cgtgtgttga tgcgtcaggg ggaggaagcg gacggacttt
      901 atgttgtggt gggtggccgc ctgcgctttg agtccgtgga tgagcgcgga gtcaaacgct
      961 caggcgactt tggccgcggc gaaatcatag gtgagcttgc acttctgact ggagatagtc
     1021 gttctgcaac agtgcgcgcg gtgcgcgatt ctgaactggt aaagctatcc gatgtttctg
     1081 tccagcgaat gcttcatgaa gccccgcacg ctctgttctg gctgacgcga atccttgcgg
     1141 agcgtctgac cagagatcaa gcggagccag tcaggcgatt ttctgtcctg accgttcttc
     1201 ccgtttcgag cggcgtcgat atgaacgcgt tctgcaccgg tttgaaagag tccctctctt
     1261 ttcacggaaa cgtggaactg atgactccac agcgcgttga tgagaaattc ggcccgggca
     1321 ccgcgagcct ggaaatggag gatccgcgcg cctccgagtt tatgatctgg ctttcagata
     1381 tagaacacgc agtcgactat cttcttctac agggaagcac ggacatgtca tggaacgagc
     1441 gttgcattcg tcaggcggat aagatcttgc ttgtcgcgga cgcaggccag gaccccaggc
     1501 tcgctcctgt ggaattgaag tatcccccgg atgcggaaca tgtttccggc gaaagaaatc
     1561 ttgtactgat ccagccggca gatgctttgc gcgcgcataa cacaaaggac tttctggcca
     1621 atcgcggtaa catgcgtcat tttcacgtta ggcgaaattc atctgccgat tttcaaaggc
     1681 tggcacgcgg attgacagga aattcaatcg gactggtcct gggcggcggt ggcgcgagag
     1741 gaatggctca cataggagtg ctgaaggctc tcatcgagca ccaggtgcca atcgacatgg
     1801 tgggtggtac aagcgccggc gccattatgg ccgggacatt cgctttcttt caagacctgg
     1861 aaagagtttc catcacagtg cgtgagttca tggtggatcg caatccgctc aacgactaca
     1921 cgtttccgtt tctctccctt gcacggggtc gaaaatactc agaagctctt gaacgggtat
     1981 tcggcaacgt gagcctggaa gatcttctgc tgcctgcttt cgctattgcc tgcaatctca
     2041 gcacctccca ggaagcatgc cttaa
//
LOCUS       DAOQJL010000642         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43374_length_2063_cov_4.641932, whole genome shotgun sequence.
ACCESSION   DAOQJL010000642 DAOQJL010000000
VERSION     DAOQJL010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43374_length_2063_cov_4.641932"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLG78_19095"
     CDS             <1..1108
                     /locus_tag="PLG78_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25289.1"
                     /translation="ARKGNDFTVYSLPDLQKLRTFSGPQAQFTQNKDPQRAIYLADGT
                     ILLNSTYWGAKLLRFHPDRPEISREESISIPHRALLRFAPAGKDDFVSFDPLTSTYAA
                     YRYANGHYYEMTKGMQEFENFSAVRWAPREQKIKMEVIIETDASAKLAELLVLLPPGE
                     TYSQTLSGEALVQGSEIIRDRHGNRYAKLFVRLEAGVSRVIVYAATLRRYLFQADLTR
                     FEDQLSRRQYPGFLKNYTADYKEFEIHNPIVESKKQEILSQAGASSRLFLEKTLSAAA
                     AIPYKGDGRFDPAPVIISQNHGTYIFHKRDFARKRSTRENRLGLAASGGRHRIQSQDS
                     GNVASWIWLDPDGIAQPSARSRNHIRSISGFRCL"
     gene            1038..1271
                     /locus_tag="PLG78_19100"
     CDS             1038..1271
                     /locus_tag="PLG78_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25290.1"
                     /translation="MESLSPPREAATTYARFLVFGAFDHGAESLTSGGDTLVKLTENG
                     KRLKTKSLRVQITLDPAEPGKSRKIRNTEEKVE"
     gene            1268..>2063
                     /locus_tag="PLG78_19105"
     CDS             1268..>2063
                     /locus_tag="PLG78_19105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE25291.1"
                     /translation="MKLDAIVIGAGPIGIEMAAALKKEGLSFAHIERGRIGETIFHYP
                     PATHFFSSPERIAIAGVPLLTQDQTKATREEYLLYLRSVVTQLDLETRLYESVESVTQ
                     AADSFQVQTRHTLTEQKTTYTARNVILATGDMHKPRMLGIPGESLPHVSHYPADPHSY
                     FRTRVLIVGGKNSAAETALRLTRAGAKVTISYRNAKLPEDSIKYWILPDLLGLIRTGE
                     IDFIGESHLLAIEPGKVKLKTRTGEIDKHFEMVLLLTGFEIDPTLYH"
BASE COUNT          598 a          533 c          472 g          460 t
ORIGIN      
        1 cgcaaggaaa ggaaatgatt tcacagtcta ttctcttccc gatcttcaga aactgcgcac
       61 attctccggt ccacaggcac aattcacaca aaacaaggat ccacagagag ccatctatct
      121 ggcggatggc accatcttac tgaactccac ttactggggc gcgaaactgc tgcgatttca
      181 tccagatcgc ccggaaatca gcagagaaga atccatttcg attccacaca gagctcttct
      241 tcgctttgct cctgcaggaa aagatgactt tgtttccttt gacccactca cctctaccta
      301 tgccgcctat cgttatgcaa acggtcacta ctacgagatg acaaaaggca tgcaggaatt
      361 cgagaacttc agcgcggtta gatgggcacc acgagaacaa aaaatcaaaa tggaagtcat
      421 catagaaacg gatgcttccg caaaactcgc agaactattg gtactcctgc ctccaggaga
      481 aacatattca caaacattgt ccggggaagc actcgttcaa ggatcggaga tcatccggga
      541 tagacatgga aaccgttatg cgaaattatt cgtccgcctg gaagccggag tttcccgtgt
      601 gattgtgtat gcggctaccc ttcgccgcta ccttttccag gcagatctga cgcgattcga
      661 agaccaactt tccagaagac agtatcctgg cttcttaaag aactacaccg cagactacaa
      721 agaatttgaa atacataacc ccatcgtgga atcaaagaaa caagaaatcc tctctcaggc
      781 cggcgcatcg agcagactct ttcttgaaaa gactctttct gcggccgcgg ccatcccata
      841 caaaggagac ggcaggtttg atcctgcccc agtcatcatt tcgcaaaacc acggaacata
      901 catatttcac aaacgcgatt ttgcgcgcaa acgatctacc cgggagaacc gtttgggtct
      961 ggctgccagt ggaggcagac atagaattca atcacaagat agcggaaacg tggcttcctg
     1021 gatttggctg gatcccgatg gaatcgctca gccctccgcg cgaagccgca accacatacg
     1081 ctcgatttct ggttttcggt gcctttgatc acggggcaga aagtctaacg tcaggcggcg
     1141 atactctggt caagctcacg gaaaacggaa agcgcttgaa aacaaaatct cttcgggttc
     1201 agatcactct cgatccggcg gagccaggaa aatcaagaaa gatcaggaac acggaagaga
     1261 aagtagaatg aaactggatg caatcgtcat cggggcggga cccattggca ttgaaatggc
     1321 ggccgcctta aaaaaagaag gattgtcttt cgcacacatc gaacgcggac ggattggaga
     1381 aactatattt cactatccgc cggccactca cttcttcagc tctccagaaa gaatcgccat
     1441 cgccggagtt ccacttctca ctcaggatca aaccaaagca acgcgagaag aatacctttt
     1501 gtatttacga tctgtggtca cccaattgga tctagaaaca agactgtatg agtctgtaga
     1561 aagtgtgacc caggcggccg attcattcca ggtgcaaact cgccatactc taacggaaca
     1621 gaaaactacg tatacggccc ggaatgtaat tcttgccaca ggagacatgc acaaacctcg
     1681 catgcttgga attcctggag aaagccttcc gcacgtcagt cactacccgg cggatcctca
     1741 tagctatttt cgcactcgag tgttgattgt aggaggcaag aactctgctg cagaaaccgc
     1801 tctgagactg actcgcgccg gcgcgaaagt gaccatcagc tatagaaatg caaagctgcc
     1861 tgaagattct attaaatact ggattcttcc ggatcttcta gggctcattc gtacaggaga
     1921 aatcgatttc atcggagagt cgcatttgct tgcgatcgag cctggaaaag tgaaactgaa
     1981 aaccagaacg ggagaaatag acaagcattt tgaaatggta ctgctcttaa ctggttttga
     2041 aattgatccg actctctacc aca
//
LOCUS       DAOQJL010000643         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43672_length_2056_cov_3.872064, whole genome shotgun sequence.
ACCESSION   DAOQJL010000643 DAOQJL010000000
VERSION     DAOQJL010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43672_length_2056_cov_3.872064"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLG78_19110"
     CDS             complement(<1..421)
                     /locus_tag="PLG78_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE25292.1"
                     /translation="MLKEDQKKQIRSVCRTILSEDADYGEYSAFLTDRTNLRGRADAI
                     CFPQSTQEVADLVRIASRERIAMVPSGGRTGYAGGAVPDGGIVVSLARMSKIDYESGL
                     LHVGAGAITADIHRRAEQEGLMFPVDFASSGSSFIGGN"
     gene            460..1536
                     /locus_tag="PLG78_19115"
     CDS             460..1536
                     /locus_tag="PLG78_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25293.1"
                     /translation="MSFLLLRRLLIAVTLVPMACVSSGKRTVEDFSSDDFQKIFRVTD
                     LDQLPKDRFLRADHYPASNERRIDLFEPKIRDLKGGYVGVGTDQNFTFIAWAKSEYAY
                     LMDFDEVVVAVNKIHLYFIEISPTFAEFKNLWDRRRQNESFEIVRRRFEKTPEWPVIQ
                     MAWKTAHRGSHDVPERLADIALMERNFGLKTFSNDPVQYNYIRDMIQRGRLIAVGGDL
                     TGTKSMHTIAKAARQLGIPIRVLYMSNAEEYFRYPETFRSNILDLPVDASSLVVRTAT
                     SGAKYEFGYPPGEKFDDVPFHYNIQPVQNLQEWMKFRRYQSILTIVRARAPLSQGFSI
                     VQKTPLEAGLKESGNIVAKPPGEW"
     gene            1541..>2056
                     /locus_tag="PLG78_19120"
     CDS             1541..>2056
                     /locus_tag="PLG78_19120"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE25294.1"
                     /translation="MKRFFFSRYAVFVFALAGCGALPFFGSPDPEVTFAVVGDIMGHE
                     AQIRSAMDPATGKYDYSPVFSYVKEQISKADLAVGNLELTLPGKPELYSGYPQFGSPD
                     ALPAALKEIGFDLLVTANNHSMDKSELGVVRTIDVLKSMGFLQTGTFTKAEYESMRIL
                     EVERNGIRMIFL"
BASE COUNT          510 a          499 c          526 g          521 t
ORIGIN      
        1 tgtttcctcc aatgaaagaa gacccgctcg aagcgaaatc gactggaaac atcagccctt
       61 cctgttctgc tctgcgatgg atgtctgcgg taatggcacc tgcacccaca tgcaggagtc
      121 cggattcgta gtctatctta gacattctgg ccagagagac tacgatgccg ccatcgggca
      181 ctgcgcctcc agcgtatccg gttctgcctc cggacggaac catcgcgatt ctttcgcgcg
      241 aggcgatccg gaccagatcg gccacttcct gtgtggactg cgggaaacaa atggcatctg
      301 cccgacctct caggttcgtt cgatcggtca gaaacgccga atactcgccg tagtctgcgt
      361 cctcagagag gatcgtgcgg cagacagagc ggatttgttt tttctggtct tccttcagca
      421 tcctggaatt tctgacgcac actcacaggg agatcgattt tgtcatttct tctattaaga
      481 cggctactga ttgccgtcac tcttgttcct atggcctgcg tttcttcggg gaagcgtacc
      541 gtggaggatt tctcctcgga cgatttccag aagattttta gagtcacgga cctcgatcag
      601 cttccgaaag atcgtttttt gcgagcggat cactatcctg catccaacga gcgccgcatt
      661 gatctatttg agccaaaaat ccgcgatcta aaaggcggat atgtaggggt tgggacagac
      721 cagaatttta cattcattgc ctgggctaag agcgaatatg cctacctcat ggacttcgac
      781 gaagtggtgg ttgcggtgaa caagattcat ctctatttca ttgagatcag ccccacgttt
      841 gcagagttca agaatctatg ggatcgcaga cgccagaatg agagctttga aattgtgcga
      901 aggaggttcg agaaaactcc agagtggcca gtcattcaaa tggcctggaa gacagcacat
      961 cgcggaagcc atgatgttcc agaaaggctg gcagacattg ctttgatgga gagaaatttc
     1021 ggactgaaga ctttttcaaa cgatccagtt caatacaatt acattcgtga tatgatccag
     1081 cgcggcagac tgatcgcagt cggcggagat cttacaggaa caaagagcat gcacacgatt
     1141 gcaaaagcag cgaggcaact tggaattcca attcgcgtgc tctatatgtc caatgcggaa
     1201 gaatatttcc gttatccgga aacgttcaga tcaaacatcc tggatcttcc agtggatgcg
     1261 tcgagtcttg tagtgcgcac tgcaacatcc ggtgcaaagt atgagtttgg atatccgccc
     1321 ggagagaagt ttgacgatgt gcctttccac tataacattc agccggtgca aaatctccag
     1381 gagtggatga agtttcgcag ataccagtcc attctcacga tcgtacgggc gcgcgctcct
     1441 ctctcgcaag gtttttccat tgtgcagaaa acgccactgg aagccggtct gaaagaaagc
     1501 ggcaacattg tggcaaagcc tccaggtgaa tggtgagtca gtgaaacgat tcttcttttc
     1561 acgttatgcg gtatttgttt ttgctctggc aggttgcgga gctctcccat tctttggttc
     1621 gcctgatcca gaagtcacct ttgctgtagt aggcgatatc atgggtcatg aggcccagat
     1681 tagaagcgcc atggatccgg ccaccggaaa gtatgattac tcgccagtgt tctcatatgt
     1741 gaaggagcag atttctaaag cggatctggc ggtgggcaat ctggaactca ctctaccagg
     1801 taagccagag ctctattcag gatatcctca gtttggttcg cccgacgcac ttcccgcagc
     1861 tttgaaagaa atcggctttg atctgcttgt gactgcaaac aatcacagca tggataaaag
     1921 cgagcttgga gttgtgcgca caatcgacgt cctgaagtct atgggtttcc tgcagacagg
     1981 cacattcaca aaagcagaat atgaatccat gagaattctt gaagtagagc gaaacggaat
     2041 tcgcatgatt ttcctc
//
LOCUS       DAOQJL010000644         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43779_length_2053_cov_3.791291, whole genome shotgun sequence.
ACCESSION   DAOQJL010000644 DAOQJL010000000
VERSION     DAOQJL010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43779_length_2053_cov_3.791291"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..331)
                     /locus_tag="PLG78_19125"
     CDS             complement(17..331)
                     /locus_tag="PLG78_19125"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HNE25295.1"
                     /translation="MTRVTFQNQKGSLRLVADGHSKKTTALDLCSAISVLTETLEASI
                     RLIAKQHSNLEKADGYFDLETEDSPAAQILFAATVLGLRSLEKQFPDALAVRVQGSMA
                     GS"
     gene            complement(328..660)
                     /gene="rplU"
                     /locus_tag="PLG78_19130"
     CDS             complement(328..660)
                     /gene="rplU"
                     /locus_tag="PLG78_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNE25296.1"
                     /translation="MSARGFTMYAIIQIAGHQHKVKENDVILTDLTGHDISKEFKCED
                     VIAVGEGESLKVGAPFVKGASVSLKVLEDTRSPKISGMHYKKRKGIRRHWGHRQDLQK
                     LQVLKIQG"
     gene            1220..1756
                     /locus_tag="PLG78_19135"
     CDS             1220..1756
                     /locus_tag="PLG78_19135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE25297.1"
                     /translation="MERVPDQLTTEYHERLIKKTRGTGTSVEGARLGMLLLWLADDMQ
                     DSVNADLEEFGITENKLGMLIILYLAASEFNRSVTPSELADLVGVERASVTGILDWLE
                     QRKFIVRKPESSDRRKIHVTISNSGKARFHRILKGYWEACDRISRHLTASEQATFEKL
                     TTKLLKGIKVDQKKKKKT"
     gene            1844..>2053
                     /locus_tag="PLG78_19140"
     CDS             1844..>2053
                     /locus_tag="PLG78_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25298.1"
                     /translation="MPVLNESRVMSRLDQRVTETLSRLHRDAEADKFVFARSIPAALG
                     AKLRGRSVFQAVAPRLKDAYMPVTPQ"
BASE COUNT          494 a          498 c          556 g          505 t
ORIGIN      
        1 ctctgaagat gggcccttag cttccggcca tgcttccctg aacgcgcaca gctagagcat
       61 ccgggaattg cttttccagc gatctgagcc ccaggacggt ggccgcgaac aatatttgag
      121 ctgcgggaga gtcttcggtt tccagatcga agtagccgtc cgccttttca agattggaat
      181 gctgcttcgc gatgaggcga atggatgctt ctagagtttc tgtgaggaca gaaatggcgg
      241 agcagaggtc cagggccgtg gttttcttag aatggccgtc tgcgacgagg cgcagacttc
      301 ctttctggtt ttgaaatgtg acgcgggtca accctggatt ttgagcactt gaagcttctg
      361 gagatcctgg cgatgccccc agtggcggcg aatgcccttg cgcttcttgt aatgcatgcc
      421 agagattttg ggagagcgag tgtcttccag aactttcaga gaaacagaag caccctttac
      481 gaaaggtgct cctactttga ggctttcccc ttcacctact gcgatgacgt cttcgcattt
      541 gaactctttg gaaatatcat gccccgtcag gtccgtgagg atgacgtcat tttccttaac
      601 tttgtgctga tgaccggcga tctggatgat tgcgtacata gtaaaacccc gagcagacat
      661 gatttttggc cccagtcggc gggtcaagcg cgtacctggg tgtttcctga cccaggtggc
      721 tgccctccct ctggagggcc aggtgctctg gcggaaactg cctgcctgga tacggtcttg
      781 cgggctacct gagttttgac ggaaatgtgc ttctatggct cggcgccgat ggctccgccc
      841 cgatggctcc gcccaatttg ccgacggtgc cggaatccct gcggtacatg gttcttgaaa
      901 ggtcgcctaa cgttattttt tggagatcag gtctgcaatt ccggttcgat tgcgctttcg
      961 cgccacgtct ttttcccgca aatctgttct tgcaccaagt gatagcagca atttgaccgc
     1021 ttctatttga tcgcatctga ccgcggatga ttccggatgc aactgcagca caactgcata
     1081 tactcaaaaa gagcacagag ctggccaggc gaattgagtt catgaagcat acgagatcga
     1141 gataaaccct tgtgcaatca agaaatgtca tccgcatttc ctgctttcct gattcaatat
     1201 gagtttgcga actgttccag tggaacgagt tccagatcaa ctgacgaccg aataccatga
     1261 aaggttgatt aagaaaacac gcgggacggg gacttcggtg gaaggtgcgc gtctgggcat
     1321 gttgcttctg tggcttgccg acgacatgca agattctgtc aatgcggacc tggaggagtt
     1381 tggaatcacg gaaaacaagc ttgggatgtt gatcatactc tacctggcag ccagcgagtt
     1441 taatcggagc gtcactccgt ccgaacttgc agatcttgtg ggcgtggagc gagcatctgt
     1501 gaccgggatt ctagactggc tcgaacaaag aaaattcatc gtccgaaagc cagagagcag
     1561 tgatcgcagg aaaatacacg taaccataag caattccggc aaagcccggt ttcacagaat
     1621 tttgaaaggc tactgggaag catgcgatcg catttcgcga catctaaccg cgtctgagca
     1681 agcaacgttc gagaaattga ccactaaact gctcaaaggc attaaagtgg atcagaaaaa
     1741 gaagaaaaaa acataaaaga ccacgcacca ccagaaggaa tacttcttgc caaatcatta
     1801 ggtaggctaa ttattaggta tactaaccat taggagtggg tgtatgcctg ttttaaatga
     1861 aagtcgggtc atgtcccggt tggatcagag agttacggaa acactgtcgc gtttgcatcg
     1921 cgacgcggag gcagacaagt tcgtgttcgc aaggtcgatt ccggcggcac ttggcgcaaa
     1981 acttcgggga agatcggtat ttcaggctgt agcaccacga ttgaaggacg cttacatgcc
     2041 tgttactcct cag
//
LOCUS       DAOQJL010000645         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_43994_length_2048_cov_3.106372, whole genome shotgun sequence.
ACCESSION   DAOQJL010000645 DAOQJL010000000
VERSION     DAOQJL010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43994_length_2048_cov_3.106372"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLG78_19145"
     CDS             complement(<1..629)
                     /locus_tag="PLG78_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25299.1"
                     /translation="MRRLRGRSGSFFLAAALCITSVQAETFRSFESAETPDALRWKLV
                     ALLNKVDSNNFQYEPETMGFAYLYQSIFTSPFNYRLYAGTVSEKQSMTMIRLEGRTGD
                     VNVFSRILEQEKLIKLDPLYTGEGKEIQYAALTPKYHWVGQPLNWVAPWLGVLHASYD
                     SPRLTTGQTVFRFSMYFGIDAVLVWAAGRNCFRDKFNAQKYAGNIAAAL"
     gene            complement(607..1197)
                     /locus_tag="PLG78_19150"
     CDS             complement(607..1197)
                     /locus_tag="PLG78_19150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE25300.1"
                     /translation="MAILSGLLVFVALASFFSLSTGWLPPGPMRPLLEKGVYGAIALA
                     SVLIYGKLLPVLFAPAPAIVARTVRTRVQIVAGITCGLALAGLLDLILAFFPEGHDPA
                     QSWQMIASGILASALEELLYRRGLQSYLSRWMPPVASVLVSSVCFAATHPMPGAFYVF
                     FCGLVFGVLYWRSGLLSAILAHGAYNFAILASAPWA"
     gene            1190..>2048
                     /locus_tag="PLG78_19155"
     CDS             1190..>2048
                     /locus_tag="PLG78_19155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE25301.1"
                     /translation="MATAKNRSYFCAVDRYLNVRASLRRRLHAVVVALVLGPLAGTAF
                     ASEIMTLPSIRSGNAIRLLRDEISQNVKSSMRGQGLAVPIRFARYTVAKDDNFYRIVT
                     STHQDPDTLASLNHLAAAADLAEGDQILIPNARGIFREEGGPMSVYIPDAKMHFFAGL
                     RFHPEESKIFRGNDFVYPLHKFIVTSSYGTRLDPFTNKQTFHGGMDLAAPEGTPVLAS
                     RPGKVVFCGNLGGYGNLVVLDHGHGYRTLYGHLKAFTVKRGAMVAAGAQIATVGSTGR
                     STGPHLHFEM"
BASE COUNT          507 a          509 c          579 g          453 t
ORIGIN      
        1 aagagagcgg ctgcgatgtt ccctgcatac ttttgtgcgt tgaatttgtc cctgaaacag
       61 tttcgtcctg ccgcccatac gagcactgcg tcgattccga agtacatgga gaatcgaaac
      121 actgtttgtc ccgttgtcag gcgcggagaa tcataagacg cgtgaagtac gccgagccag
      181 ggtgccaccc agttgagtgg ctggcctacc cagtggtatt ttggagtgag ggctgcgtac
      241 tggatttcct ttccttctcc tgtatagaga gggtccagtt tgattaactt ttcctgttcg
      301 agtatgcgcg agaaaacgtt cacgtcacct gtgcgccctt ccaggcgaat catggtcatg
      361 gattgttttt cgcttacggt ccccgcgtag agtctgtaat tgaatggtga agtgaagata
      421 ctctggtaga gataggcaaa gcccattgtt tccggttcgt attggaagtt gttgctatct
      481 actttgttga gaagcgccac gagtttccag cgcaacgcgt ccggggtctc ggcggattca
      541 aaggatcgaa atgtttccgc ctgcacggat gtgatgcaga gtgcggcagc cagaaagaat
      601 gagccgctac gcccacggag ccgacgcaag tatggcgaag ttgtaagcac cgtgtgccag
      661 aatcgccgaa agcagaccgg agcgccagta taaaaccccg aagaccagcc cgcagaaaaa
      721 cacgtagaac gctccaggca ttggatgcgt tgccgcaaaa caaacggaag aaacgagaac
      781 ggaagcaacc gggggcatcc atcgggaaag gtaactctgc aatcctcttc tatacaagag
      841 ctcctcgagt gcagacgcaa gaattccgga agcaatcatt tgccagcttt gagctggatc
      901 atgtccctca ggaaagaaag caaggatcag atccagcaat ccggcgaggg ccaggccgca
      961 tgtgatgcct gctacaatct ggacccgtgt gcggacggtt ctggcgacga tggccggtgc
     1021 cggtgcaaat aggactggga gtagcttacc gtagatgaga acggatgcca gagcaatggc
     1081 accatacacg cccttctcca ggagtggtct catcggaccg ggaggaagcc agccagtgga
     1141 cagggaaaaa aaagaagcca gggccacaaa aacaaggagt ccagagagga tggccacggc
     1201 caaaaatcga tcatacttct gtgccgtaga tcgatattta aatgtgcgcg cgtctcttcg
     1261 cagaaggctt catgctgtag ttgtcgccct cgtgcttggt ccgcttgccg gcaccgcgtt
     1321 tgcgtctgaa atcatgactc ttccaagcat tcgcagcggg aatgcgattc gtcttttgcg
     1381 agatgagatt tcgcagaatg tgaaatcatc gatgagggga caggggctgg cagtgccgat
     1441 tcgctttgcg cgatacactg ttgcgaaaga tgacaacttt tatagaatcg tgacgagcac
     1501 gcatcaggat ccggacactc tcgcatccct caatcatctc gcggccgccg cagacctggc
     1561 agaaggcgat cagatcctca ttcccaacgc ccgcggcatt ttcagagaag aaggcggacc
     1621 tatgtccgta tatattccgg atgcgaagat gcatttcttc gcaggcctga ggtttcatcc
     1681 ggaggagagc aagatctttc gggggaatga ttttgtttat cctcttcaca agttcattgt
     1741 aacctcttcg tacggaacac gactcgatcc attcacaaac aaacaaactt ttcacggagg
     1801 aatggacctg gcggcaccgg aagggacacc ggttctggcg agtcgcccgg gcaaagttgt
     1861 gttttgtgga aacctcggag gctacggcaa tctggttgtg ctagaccacg gtcacggtta
     1921 cagaacgtta tacggtcatc taaaagcatt cacagtgaag agaggagcga tggttgcggc
     1981 cggggcgcag attgcgacgg ttggatctac tggacgatcc accggaccac atttgcattt
     2041 tgaaatgc
//
LOCUS       DAOQJL010000646         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_44027_length_2047_cov_5.274096, whole genome shotgun sequence.
ACCESSION   DAOQJL010000646 DAOQJL010000000
VERSION     DAOQJL010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44027_length_2047_cov_5.274096"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLG78_19160"
     CDS             complement(<1..1150)
                     /locus_tag="PLG78_19160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE25302.1"
                     /translation="MIDPDRRISAGASLNSPHPPRDLAKYASQRLLRHLAENPGSPDE
                     PVSRSFAGAVLFADMQNFTGLAERLNTEDPANGVQKLAQYLDIYIGKLVDIITANGGD
                     IVKFAGDAVFAIWENENAMSQSLLAAIHCGLVIQKELHRFEVAPGELLALRVGVSAGN
                     MHELHLGGYRDRWEFLIAGLPMSESGQSATHADPGQVIVSPAAARVLSGSGLAKRHGK
                     KLLVRPAFPAELPGPLKRSDIVEGAEDAFSVYVPRALLVRSGNMRAELRPVTVLFCKV
                     RGFKFSAETSLAAVQVVMLVMQECVYRYEGSINRFGVDEKGAILLAAFGLPPLVHEDD
                     PLRGIQAARDIRTALAAIGHDASIGVATGRAFCGTVGNNTRCEYTMHGV"
     gene            complement(1169..>2047)
                     /locus_tag="PLG78_19165"
     CDS             complement(1169..>2047)
                     /locus_tag="PLG78_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25303.1"
                     /translation="IRLTFIFRRAKCSLDSGNMGFLDWFKLGKEPDKRIELSGLDEVE
                     LAEELHGGPLVRDTPVPRIFAKFAESEIHELMAWSGIFDALKEKGYDRTELELQYLSD
                     QDQRIFVKEKGEILIHIRLKLSSFRFRLHPGAPQRKLLYIDWLMTRHPRIRSYKKEKL
                     FPGQELPGLGIFTHVTDFITNLALGVGAKGAFNIPEYFHDALLFHRAFQFYDPVREAH
                     FRALIRDLRKFGARAISHALAEGRIRNSEGEVLAWSPGEMISILDPELEEMIWNPEYY
                     TRVVRELKRVSYTMSS"
BASE COUNT          478 a          539 c          518 g          512 t
ORIGIN      
        1 tcactccgtg catcgtatac tcgcagcgtg tgttgtttcc cactgttccg cagaacgcac
       61 ggcctgtggc cactccaatc gatgcgtcgt ggccgatggc agccagggct gttcgaatgt
      121 cgcgcgctgc ctgaatgccg cgtaacgggt catcttcatg aaccagcggt ggaaggccga
      181 atgcggccag gaggatggct cctttttcat ccacaccaaa tcgattgatg cttccctcgt
      241 aacgatacac acactcttgc atgaccagca tgactacctg caccgccgcc agagatgttt
      301 ccgccgagaa tttgaatcct ctaactttgc aaaaaagtac agtcacaggt cgcagttctg
      361 cgcgcatatt tccacttcgc acaagaagtg cgcgcggaac ataaacgctg aatgcatcct
      421 ccgcaccttc tacaatatca gatctcttga gtggaccagg aagctccgct ggaaacgcgg
      481 gacgaacgag caattttttt ccgtgccttt ttgcaagtcc agagcctgac aggactctgg
      541 ctgctgccgg agatacaatg acctgtccag ggtctgcatg tgtggcagac tgtcctgatt
      601 cgctcatggg aaggcccgcg attagaaact cccatctgtc tctgtatccg cccaggtgaa
      661 gttcgtgcat attgcccgcg cttactccaa cacgcagtgc cagtagttcg cctggagcca
      721 cttcgaaacg atgcagttct ttttgtatga cgaggccgca gtggatggca gcaagtagag
      781 actgcgacat tgcattttcg ttttcccaga tggcaaagac tgcgtcgccg gcaaacttta
      841 caatgtcgcc tccgttggca gtgatgatgt ctacgagttt tccaatgtaa atgtccaggt
      901 actgtgcgag cttctggact ccgtttgccg gatcttctgt attcagcctt tctgcgagtc
      961 cggtgaagtt ctgcatgtct gcaaacagaa ccgctccggc aaagcttctg gagaccggtt
     1021 catccgggct gcctggattc tctgcgaggt ggcgcagaag tctttgcgaa gcatacttcg
     1081 caaggtctct gggaggatga ggactgttca aagatgcgcc tgctgagatg cggcggtctg
     1141 gatcgatcac ggtcaagcca gaaatccttc aactggacat ggtgtaagag actcgtttga
     1201 gctctctaac cacacgagtg tagtactccg gattccagat catttcctca agctcaggat
     1261 ctagaatgga tatcatttcc ccaggactcc aggcaagcac ctctccctct gagttacgga
     1321 ttcttccttc cgccagagcg tgcgaaatgg cccgtgctcc aaattttctg aggtcgcgga
     1381 tgagggcgcg aaagtgtgct tctcgaaccg gatcataaaa ctgaaacgcg cgatgaaaga
     1441 gaagagcatc atgaaagtat tctggaatgt tgaaggcacc ttttgctcca actccaagcg
     1501 ccagattcgt gatgaagtcc gtgacatgag tgaagattcc aagaccaggc aattcctgcc
     1561 cgggaaagag tttttctttt ttgtaggatc gaatccgcgg atggcgagtc atcagccaat
     1621 caatgtagag tagttttctc tgcggcgcac ctggatgcag gcgaaatcga aaagaagaaa
     1681 gcttgaggcg aatgtgaatg agaatttcgc ctttctcttt tacaaagatt ctttgatcct
     1741 gatcggagag atactgtagc tccagctcag tgcgatcgta tcctttttct ttcagagcgt
     1801 caaagatccc ggaccacgcc atcagctcgt gtatttcaga ttctgcgaac ttcgcaaaaa
     1861 tgcgcggaac cggagtatct cgaaccagcg ggccgccgtg cagttcttct gccagttcaa
     1921 cttcgtctag accggagagt tcaattctct tgtccggctc cttgcctaat ttgaaccagt
     1981 ctagaaatcc catattacct gaatcgagcg agcacttcgc acgtctgaaa atgaaggtca
     2041 atcgtat
//
LOCUS       DAOQJL010000647         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_44146_length_2044_cov_4.745098, whole genome shotgun sequence.
ACCESSION   DAOQJL010000647 DAOQJL010000000
VERSION     DAOQJL010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44146_length_2044_cov_4.745098"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLG78_19170"
     CDS             <1..540
                     /locus_tag="PLG78_19170"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNE25304.1"
                     /translation="SQDGNNARAMSGQCFYKGPGKIRFDFDNPAGNLIVSDGRTMWVY
                     IRRLNAVGKQDLRLQKKDESGKNIFQDTAGPGVSRLFRKYHYRFDRPEQPRNEDGSSV
                     FVLDMDQREKTGGYEKIKLYVDASSYLIRKAVASDSQGKSTTLVFRNPALNPPLEGKL
                     FQYDPPESVRVVPNPLVSE"
     gene            545..1627
                     /locus_tag="PLG78_19175"
     CDS             545..1627
                     /locus_tag="PLG78_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE25305.1"
                     /translation="MLAKRLGTILKDAREAKKLSIKDVARETFITARWVEALENEDFS
                     VFPAETYALGALKSYSEFLNLETSHLENLFRGQQIDLSQSPIEELTRPTGIRMPAIGK
                     NVVFVIAGLAVGLGLLLLVMSGALKFSSGTGSSSAHSSEANCHDREIIPVTLPARGAP
                     PRQEAVSRENAIRFNVDSTALKICLGEVQQTPEGKARALFLMRINDESDFSFYAAEGE
                     MVALSSDTQALKELSGEIRITPKAIGEASARIQLESGEKGAQPQGAIQVTLQFVADSY
                     VEWTDDGNYHRGVEIRAGESRTFEAKSRLEVKVGNGAGVQVIAPGKPPRIAGPPGKIV
                     KITYRKVPDPLDPGLAQIEEKIEAAQ"
     gene            1624..>2044
                     /locus_tag="PLG78_19180"
     CDS             1624..>2044
                     /locus_tag="PLG78_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25306.1"
                     /translation="MKSPSYYITTLGCPKNQADSREMERSLSQEGYIRTDSADQADVH
                     LINSCAFIEDARVETIRTVLDAGKVKKKHKKQKLVLVGCFTERYADTVRAELPEIDFS
                     FGTGIYHKAGELIRNAFGLSVGLEPVSPFFEMQAQGKP"
BASE COUNT          555 a          474 c          539 g          476 t
ORIGIN      
        1 agtcaggatg gaaacaatgc acgcgctatg agcggtcagt gcttctacaa agggcccgga
       61 aagattcgct tcgattttga caatcctgcg gggaacttga ttgtttcaga cggtagaacc
      121 atgtgggtct acattcgaag gttgaacgct gtagggaaac aggaccttcg tcttcaaaag
      181 aaagatgaaa gcggaaagaa tattttccag gacacagcag gtccaggcgt ttctagattg
      241 tttagaaaat accactatcg ttttgacagg cctgaacagc cgcgcaacga agatggaagc
      301 agtgtttttg ttctggatat ggaccagaga gaaaaaacgg ggggatacga gaagatcaag
      361 ctgtacgtgg atgcttcgtc atacttgatt cggaaagccg ttgcttccga tagtcaggga
      421 aaatccacaa ctctcgtatt tcgcaatcca gcattgaatc cgccgctgga aggaaaactt
      481 ttccagtatg atccgccgga atccgtgcgg gtcgttccca atccccttgt gagtgaatga
      541 ttgaatgtta gctaaaagac tgggcactat tctaaaagac gcacgtgaag caaagaagct
      601 ctccataaaa gacgttgcac gggaaacgtt catcaccgcg cgatgggtgg aagcactgga
      661 gaatgaagat ttctccgtct tcccggcaga gacttatgca ctcggtgctt tgaaatcgta
      721 ttccgaattt ttgaatctag aaacatctca tctggaaaat cttttccgtg gccagcagat
      781 tgatttgtct cagtctccca ttgaagaact caccaggcct actgggattc gtatgcctgc
      841 gatcggcaag aacgtggtgt ttgtcattgc aggtcttgcg gttggtctgg gtcttctgct
      901 tctggtgatg tctggcgcgc tgaagttttc gagcggaaca ggttcatcat ccgcacattc
      961 ctctgaagca aattgccatg accgcgaaat catccctgtg actcttcctg caagaggggc
     1021 tcctcctcgt caggaggcag tgtcccgcga aaatgcaatc cgcttcaacg tagatagcac
     1081 tgctttgaaa atttgtctgg gtgaagtgca acaaactcca gaaggaaaag cacgcgcgct
     1141 ctttttgatg agaatcaatg acgagtccga tttttccttc tatgctgcgg aaggagaaat
     1201 ggtagcactc agttcagata cgcaagctct caaagagctc agcggtgaaa ttcggatcac
     1261 tccaaaagca atcggagaag cttccgctcg cattcaactg gaatcaggcg aaaagggagc
     1321 gcaaccgcaa ggcgccattc aggtaactct gcagttcgtc gcggattcat acgtggaatg
     1381 gaccgacgat ggaaactatc accgtggcgt agaaatccgc gcaggggaat cacgcacatt
     1441 tgaagcaaaa agcaggctgg aagtgaaagt aggaaacgga gccggagttc aggtcatcgc
     1501 tcctggcaag cctcctcgca ttgcaggtcc acctggcaaa attgtaaaga ttacttacag
     1561 aaaagttcct gatccgctcg atccggggct tgcgcagatc gaggagaaga tcgaagcggc
     1621 gcagtgaaat ctccgtcgta ctacatcacg acgctcggct gtcccaagaa tcaggcagac
     1681 agccgggaaa tggagcgaag tctttcgcag gaaggataca ttcgtacgga ctctgccgat
     1741 caggcagatg tgcatctcat caattcctgt gctttcattg aagatgcaag agtggaaacc
     1801 atccgcacag tactcgacgc gggcaaagtc aaaaagaaac acaaaaaaca aaagctcgtt
     1861 cttgtaggct gctttacgga acgttatgcg gacaccgtgc gcgctgaatt gccagagatt
     1921 gattttagct ttggaaccgg gatctatcac aaggcaggcg aactgatccg aaacgcattc
     1981 gggttgtctg tgggtctcga gcctgtttct ccttttttcg aaatgcaggc gcagggaaag
     2041 ccgt
//
LOCUS       DAOQJL010000648         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_44239_length_2042_cov_4.306492, whole genome shotgun sequence.
ACCESSION   DAOQJL010000648 DAOQJL010000000
VERSION     DAOQJL010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44239_length_2042_cov_4.306492"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="PLG78_19185"
     CDS             <1..1411
                     /locus_tag="PLG78_19185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNE25307.1"
                     /translation="DFRPEYRALHNLRISGVPVVALTATATARVTRDVISSLDLHDPE
                     IIKKSFFRPNLHYRVLHPENDREKQRILLDLLEERKFRENAPGKCIIYCATRKKVDQV
                     FEFLGSYGFRVGRYHAGRTSAGREKMQDAYSAGKVNVLVATNAFGMGMDALDVRLVLH
                     YQVPSSLDAYYQESGRAGRDGQVSDCILFFSNADFVTQSFILGRSAGEDLLAPVRDFG
                     YSQMCRQQYICSYFGEEISSCGVCDCCLEDGSASREDFLERERKSQTHRDARASYEFS
                     DSEIESVHAVLRDYPGKFGKKILAGVLRGSKARDIIRYRLTQSSAYGALKDVPEEAII
                     RFFQDGIDNGIIKIEGIKYPKIYLAQEPPRARVRKESPRKSGPTEASDLLKKLKSFRD
                     REARRLKWKKYMVMQNGLLSRIALSRPSSFAELARIKGMGDSRAEKYGPEILRIISES
                     LYSAGVQGKSIHGTSRNQS"
     gene            1386..>2042
                     /locus_tag="PLG78_19190"
     CDS             1386..>2042
                     /locus_tag="PLG78_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE25308.1"
                     /translation="MGLRATNLEKLDKMTFDVLVLGGGINGAISAAVLAARGARVALI
                     DRADFASFTSQESSNLVWGGIKYMETLEFGLVRNLCMSRNHLIRSYPSTVQEIRFFVN
                     LEKGFRWPRLFLFMGSLLYWVIGNFFTKGPRLLSKADIEREEPVVRQDNSMGGFEYSD
                     AYLHDNDARFVFGFVRTALDNGAIAANYVESLGSKKDGQFWNTQARDVLTGRTFDIKS
                     R"
BASE COUNT          505 a          475 c          550 g          512 t
ORIGIN      
        1 cgatttcagg ccggagtatc gcgctcttca taatctgaga atttccggtg ttcctgtagt
       61 cgcactcact gcaactgcaa cggcgcgcgt aaccagagat gtaatttcgt ctctggatct
      121 tcatgatcca gagatcatca agaaaagttt ttttcgacct aaccttcact accgtgtttt
      181 gcatccagaa aatgatcgtg agaagcaaag aatcctgctc gatctactcg aagaaaggaa
      241 gtttagagag aatgcgccgg gcaagtgcat catctattgc gctactcgaa agaaagtgga
      301 ccaggtattc gaattccttg gttcctatgg ctttcgcgtc ggtcgctacc acgctggcag
      361 aaccagtgcc ggtcgtgaga aaatgcagga tgcttattcc gcggggaaag tgaacgtact
      421 tgtggccacg aacgcatttg gaatggggat ggatgcgctg gatgttcgcc tggtcctgca
      481 ctatcaggtg ccttcttctc tggacgcgta ctatcaggaa tccggccggg cgggaagaga
      541 cgggcaggtt tcagattgta tacttttctt tagtaatgct gattttgtaa cgcagagttt
      601 catcctgggc cggagcgcgg gagaagatct tctggcacct gttcgtgact ttggttattc
      661 gcagatgtgc agacaacagt acatttgttc ctactttgga gaagaaattt cttcctgcgg
      721 tgtctgcgat tgttgcctgg aagacggctc tgccagccgc gaagattttc tagaaagaga
      781 aagaaagagt caaacacacc gcgacgcgcg tgcttcttac gagttcagcg attctgaaat
      841 tgaatccgta catgcggtgt tgcgcgacta tccaggaaaa tttggaaaga aaatactggc
      901 cggcgtgctt cgcggaagca aagcacgcga catcattcgc tatcgcttga ctcagtccag
      961 tgcctatgga gcactcaagg atgttccgga agaagccatc attcgtttct ttcaggatgg
     1021 aatcgacaac gggattataa aaatagaagg gatcaagtat ccaaagattt acctggccca
     1081 ggagccgccc cgtgccagag tcaggaagga atcgcccaga aagtcaggtc caacggaagc
     1141 atccgacttg ctcaagaagt tgaaatcttt cagggacagg gaagctcgtc gtttgaaatg
     1201 gaagaaatac atggtcatgc agaacggttt gctttcgcgc atcgctctgt cccggccgtc
     1261 ttcatttgct gagctcgctc gcatcaaggg aatgggggat tcgagagcag agaaatatgg
     1321 ccctgaaatt ctacgcatca taagcgaatc gctttactcc gctggagtcc agggaaaatc
     1381 gattcatggg acttcgcgca accaatcttg aaaaactcga taagatgacg ttcgatgttc
     1441 tcgttctggg tggcgggatc aacggggcaa tttctgcggc agttctggct gccaggggtg
     1501 cgcgagttgc gctcatagac agagcagact tcgcatcttt caccagtcag gaatcgtcca
     1561 atctggtctg gggtggaatc aaatacatgg agaccctgga gttcgggctc gtgcgcaatc
     1621 tgtgcatgtc tcgcaatcat ctgattcgca gttatccgtc cacggtgcag gaaattcgat
     1681 tctttgtgaa tctggagaag gggtttcgct ggcccagact atttctgttc atgggttcgc
     1741 ttctttactg ggtgattgga aatttcttca cgaaaggccc gcgtcttctt tcaaaagcgg
     1801 acattgaaag agaagaaccc gtggtgcgcc aggacaacag catgggcgga tttgaatatt
     1861 cggatgcata ccttcacgac aacgatgctc gctttgtttt tggttttgta cgcacggctc
     1921 tggacaacgg tgccattgcg gccaactatg tggagtccct tggatcaaaa aaggacggcc
     1981 aattctggaa cacgcaagcg cgcgacgtgc tcaccggacg cacattcgac attaaatcta
     2041 ga
//
LOCUS       DAOQJL010000649         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_44460_length_2037_cov_4.250252, whole genome shotgun sequence.
ACCESSION   DAOQJL010000649 DAOQJL010000000
VERSION     DAOQJL010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44460_length_2037_cov_4.250252"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PLG78_19195"
     CDS             complement(<1..566)
                     /locus_tag="PLG78_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE25309.1"
                     /translation="MSTLSCTLACIECNTSYDIHQVRYLCDCGGLLEVRHDMAAIRSR
                     SALDWKTLFTERYRSSEYPFQSGVWGKKEWVLPHISNAEIVSLGEGATPMVGIPRLAR
                     ELGLGSLSVKQCGVSHTGSFKDLGMTVLVSHVVSLRAKGIPIHAVACASTGDTSAALA
                     AYAASAGIPTVVFLPAGKVSNAQLVQPVS"
     gene            complement(563..1204)
                     /locus_tag="PLG78_19200"
     CDS             complement(563..1204)
                     /locus_tag="PLG78_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25310.1"
                     /translation="MILLIIPFALNFAVTTAGVLALVGLVVLFYVLIVRPAMLIRADR
                     EAPPPEEGSVREIIVDPSLRALKLSIGQIDSDLKTRMNGIREDHIVFRFTKDRELEDY
                     EIQINATGAAQIRVPHSKKMENFVGMERLHSSELIGDSAGFRLVASAREGRVNQYVEF
                     ELTAKYFITRAGDERMKFLLTLKKIFPSLDKNSRNKKGIYTFGRVKLGQQVEE"
     gene            complement(1201..1773)
                     /locus_tag="PLG78_19205"
     CDS             complement(1201..1773)
                     /locus_tag="PLG78_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25311.1"
                     /translation="MQKKAVLILVTLVACASPQKPVSVNQTTQKVNPADDPFFKEPNP
                     AGETFRVLILNDSYTVRQMSGDTDLVKKQDDSGDKEQLNTFQELAARYDFKDWDFQGL
                     LSVRLHPHRGEIEHIEYVLGQNPKTWQASKLFQEDVSRFQFQFPKGQVTLRDFRVRYR
                     WKIQRRPGLTDEEARKRALEFLKAETLQNH"
     gene            complement(1752..1991)
                     /locus_tag="PLG78_19210"
     CDS             complement(1752..1991)
                     /locus_tag="PLG78_19210"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE25312.1"
                     /translation="MIETQSGALYTGITTDVQRRFEQHSSGKGARFFRTSKPVRIVHV
                     EPGLDRSTALSREAAIKQLTVHEKRRLIRCKKKRF"
BASE COUNT          512 a          527 c          494 g          504 t
ORIGIN      
        1 gacactggct gcacaagttg cgcattggac actttgcctg ctggaagaaa aacaaccgtt
       61 gggattccgg cggaagcagc atacgccgcg agagcagcac tcgtgtctcc cgtagaagca
      121 caggcaactg catgaattgg aattcccttt gcacgaagag aaacaacatg acttacaagc
      181 acagtcatac ccagatcctt gaatgatcct gtatgactca ccccacattg ctttacggaa
      241 agcgaaccaa gccccagctc acgcgcaagg cgagggatac ccaccattgg agtggcacct
      301 tctcccagag acacgatctc cgcatttgaa atgtgaggaa ggacccattc cttctttccc
      361 cagactccgg actgaaaagg atattcagaa gagcggtatc gttctgtaaa caaagtcttc
      421 caatctaaag ccgacctgga acgtatggcc gccatatcgt gacgaacttc gagcaatccc
      481 ccgcaatcgc agaggtagcg aacctggtga atgtcgtatg acgtattgca ttcaatgcat
      541 gcaagtgtgc aactgagcgt gctcattctt caacctgttg tcctagcttt acacggccga
      601 acgtatagat acctttctta ttgcgtgaat tcttgtcgag cgaagggaaa atctttttca
      661 aggtcaaaag aaacttcatc ctttcatctc cggcacgggt gatgaaatat tttgcggtga
      721 gttcaaactc aacgtactga ttgacccgac cttcacgagc actggcaacc agtcgaaagc
      781 cggcagaatc gccaatcaat tcgctcgaat gaagtctttc catgcccacg aagttttcca
      841 ttttctttga gtgtggaact cgaatctgcg cggctccggt tgcattgatc tggatttcgt
      901 agtcttccag ctctctatct tttgtgaagc gaaacacaat gtggtcttcg cgaattccgt
      961 tcatccgcgt cttgagatct gagtcaatct gcccgattga aagtttcagc gctcggagag
     1021 atgggtccac aatgatctca cgaacagatc cttcctctgg agggggagct tctctgtctg
     1081 cgcgaatgag catcgccggt ctaacaatca gtacgtaaaa aagtacaacc aggccgacca
     1141 gggcgagcac gcccgcggtg gtcacggcaa aattcagggc aaaaggaatg atgagcagaa
     1201 tcaatggttc tgcagagtct cagctttcag aaattcaagt gcccgcttgc gtgcctcttc
     1261 atccgtgagg cctggacgac gttgtatctt ccagcgatag cgaacgcgaa aatcacgaag
     1321 tgtgacctgc ccttttggaa attggaactg aaatctggac acgtcttcct ggaagagttt
     1381 ggacgcctgc caggtctttg gattctgacc aagtacgtat tcgatgtgtt cgatttcgcc
     1441 tctgtgagga tgaagacgca cagatagcag accctggaaa tcccagtctt tgaaatcgta
     1501 acgcgcggcc aattcctgga aagtattcag ttgctctttg tcgccggagt catcctgctt
     1561 ttttaccaga tcagtatccc ccgacatctg acgcacagtg taagaatcat tcaagatgag
     1621 aacgcgaaat gtttcaccgg ctggatttgg ctctttgaaa aacggatcat ccgccggatt
     1681 cactttctga gtcgtctggt ttactgaaac cggcttctgt ggagaagcgc acgcgaccag
     1741 tgtgacaagg atcagaaccg cttttttttg catcgaatca gcctccgttt ctcatgcaca
     1801 gtgagctgct tgatcgccgc ttcgcgcgaa agcgcagtgc tacgatcgag gccaggttcg
     1861 acatgcacaa tgcgcacagg tttcgatgta cgaaagaagc gcgcaccttt tccggaactg
     1921 tgttgttcaa acctgcgttg aacatcggtt gtaatgccgg tgtacagcgc cccgctctga
     1981 gtctctatca tatagacact ccagctgctc acagaatgca tggcgtgatc ttattcg
//
LOCUS       DAOQJL010000650         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_44655_length_2032_cov_4.196763, whole genome shotgun sequence.
ACCESSION   DAOQJL010000650 DAOQJL010000000
VERSION     DAOQJL010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44655_length_2032_cov_4.196763"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..523)
                     /locus_tag="PLG78_19215"
     CDS             complement(221..523)
                     /locus_tag="PLG78_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNE25313.1"
                     /translation="MKAAASKRELDKPEWTFLTNHAHVLLCLHRQPEMRMRDVAMLVG
                     ITERAVQAIVRDLEKVKCLRKEKKGRRNSYEIRTEIALRHPLENRKTVGDLLKAVK"
     gene            complement(622..>2032)
                     /locus_tag="PLG78_19220"
     CDS             complement(622..>2032)
                     /locus_tag="PLG78_19220"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNE25314.1"
                     /translation="ETIRRHIEEQDSRVKTIGFAGFFGVPLAYRKLEERDTSLRMPVL
                     LSPAFTVKEVPRSPDRYRTEDGPTDVFVLSYFRNLRKSALSSFLFVELFGILSIENIV
                     RKTLVSIIRRLKGKAIPARFDDRKYRPAQETLSPDGNPEGMEVRVQRAITILKRMGLR
                     EPFGRLVFIVGHGSVTNNNAFGSALDCGACGGHAGDINARYLADLLNDSEVRVRLAQL
                     GIQIAQTTWFVAAVHETVTDEVFFLDEETIPVAFKMDLEMVRGILNSASSRARAERQK
                     AFKNLSVSSAQTRGQNWSEVRPEWGLAGNACFIVAPRERTRGVNLGSRAFLHDYNYAI
                     DDEFKTLELIMTAPMVVTNWINMQYYASTVANGVYGAGSKVLHNLVAEMGVLEGNGGD
                     LRVGLPLQSISDGKDILHEPLRLSVFIEAPRDAIEDIVKRHETVRQLLDHEWLHLLQI
                     DPSDGQVWRRLPGGDYTVV"
BASE COUNT          468 a          539 c          506 g          519 t
ORIGIN      
        1 gaaactgggc cgacttctca gagaggtttg catggtgtga tgtttcacca agtcctgtgc
       61 agatcagctg gaccccgagc tgttgaaggt gccgggcaat tgccagggcg agggaagagc
      121 ggtctgtgat tcccgcgatc aggattgatt tgccatacca tgatgaattc atttcaccat
      181 agtatatacg aagaaatttt cgcctattat ttttcgtgaa ttatttcaca gcttttaaca
      241 aatctccgac tgtcttacgg ttttcgagcg gatgccgcag tgcaatttcc gttcttattt
      301 cataggagtt acggcgccct ttcttttctt tgcgcagaca cttgaccttt tcgagatccc
      361 tgacaatcgc ctgaacagcc ctctctgtaa ttcctacgag catggctaca tccctcatcc
      421 gcatctctgg ctggcggtga aggcagagga ggacatgcgc gtggttcgtt agaaacgtcc
      481 actctggttt gtccaattcc cgttttgaag ctgcggcttt cattgccgaa gagcgtaaca
      541 aatgccctgg gcgacgtcaa gcgcgatcca cccgaaaggc gggtcgcgcc tcgaatacgc
      601 gaatctcatt tcacgaatat cttaaaccac agtatagtca ccgcctggca ggcgtctcca
      661 gacttgtcca tctgaaggat cgatctgcag caaatgcagc cattcgtgat cgagtagctg
      721 ccgtaccgtt tcatggcgtt ttacaatatc ctcgatcgca tcgcgtggag cctcaatgaa
      781 tacgctcaga cgcaagggtt catgcaagat gtcctttccg tcacttatgg actgcagagg
      841 aagtccgact cgtagatcac ccccgttgcc ttcaagaaca cccatttcgg ccacgagatt
      901 gtggagtact ttgctgcctg caccgtagac accgtttgcc acggtcgagg cgtagtactg
      961 catgttgatc cagttcgtga cgaccatggg agcagtcatg attagttcca gtgtcttgaa
     1021 ctcgtcatcg attgcataat tgtagtcgtg aagaaaagct ctactgccca gatttactcc
     1081 gcgcgtccgc tctcgtggcg cgacaatgaa acatgcgtta ccggccaggc cccattccgg
     1141 gcgaacttca gaccagttct gtccacgcgt ctgagcgctt gagacgctca aattcttaaa
     1201 cgctttctgg cgctcagcac gagcacgcga cgacgcactg ttgagaatac cccgcaccat
     1261 ttccagatcc atcttaaacg caacgggaat tgtttcttcg tctagaaaga aaacttcgtc
     1321 ggtaacagtt tcgtggactg cggcgacaaa ccaggttgtt tgcgcaattt ggatgccgag
     1381 ctgcgcgagt ctcacacgga cttccgaatc gtttagcaag tcagcgaggt accgtgcatt
     1441 gatatcgcct gcatgtccgc cgcatgctcc acagtcgagt gcagatccaa acgcattgtt
     1501 attggtaacg cttccatgcc caacaatgaa cacaaggcgg ccgaaaggtt cgcgcagacc
     1561 catacgcttg agaatcgtga tggcgcgttg aacgcgtact tccatgcctt ctggatttcc
     1621 gtcaggcgaa agcgtttcct gggccgggcg atatttccga tcatcgaatc ttgcggggat
     1681 ggcttttcct ttcaatcttc tgatgattga gaccagcgtt tttcgcacga tgttttctat
     1741 ggaaagaatg ccgaagagct cgacaaaaag aaatgaagag agagcgctct tgcgcaaatt
     1801 ccgaaagtag ctcaatacaa atacgtcagt cggaccgtct tccgtgcggt agcgatccgg
     1861 cgacctggga acctctttga ccgtgaaagc aggactcagt agtacaggca tccgcaggct
     1921 cgtgtctctt tcttccaact ttctgtaagc aaggggaacc ccgaagaacc cggcaaaacc
     1981 aatcgttttt acgcgcgagt cttgttcttc aatgtgtcgg cgaatggttt cg
//
LOCUS       DAOQJL010000651         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_45504_length_2012_cov_3.775166, whole genome shotgun sequence.
ACCESSION   DAOQJL010000651 DAOQJL010000000
VERSION     DAOQJL010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45504_length_2012_cov_3.775166"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1212
                     /locus_tag="PLG78_19225"
     CDS             160..1212
                     /locus_tag="PLG78_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25315.1"
                     /translation="MLASASTVSQNPVKSVYSFVTNVATNPTLMTVPAVDTARAFVMC
                     SYRLANTSFQTNRVPICSLTSSTQVTVQAGTSDAGVLVRGYVVEFSTGVNVQRGAFTL
                     AGGTLNNTVSISPVSQNRSFVIVQSRTTDTGTLIDEHRTVMGYLQSSSSLYVVRNQAT
                     IITDVEYQVIEMQGATVQYGISQIPGGSFSVSIPIAAVNTSKSFVVFSSAADNLSNGN
                     DSEMAVRCDLANASQLLCQRQDSTNAPDIAWYVVSFADSTYVERGSADTNVFTPPANN
                     TDTSMTVTPSVPFDLTRTLPIGSTASDPGDADAGLDSASFTQHLLTSTQLNIQRGSAD
                     DRRSVVSWQLIQFPAR"
     gene            complement(1249..1605)
                     /locus_tag="PLG78_19230"
     CDS             complement(1249..1605)
                     /locus_tag="PLG78_19230"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE25316.1"
                     /translation="MSNSAELEISEILPFEKGFRVNISGPINIYSAGQLTNAIIKKTT
                     DGMKLAVDLSKVPMVDSSGIGFLIRLARDLEKKKGKVILCGVTDDFKSNLTALRVDKL
                     VRLASSAATIPADFWH"
     gene            complement(1602..>2012)
                     /locus_tag="PLG78_19235"
     CDS             complement(1602..>2012)
                     /locus_tag="PLG78_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25317.1"
                     /translation="KVSAKEAPEVKFVSEIKTEIEKREALIEATPAREEPPPGISVVL
                     SDGRTLQGFSILMEDDRAILAGSRPVAVPAILIASIRLAPSRTDYNVCAIDRSRHVLC
                     GEAIEGKNQEFAVGGDSMPLTSIEAILVQKRGVQ"
BASE COUNT          464 a          507 c          513 g          528 t
ORIGIN      
        1 tccggaaatg tttgcagaat cgcctaacga agtgttaagc gttatgcctt gagcgatgta
       61 aaaccagggg atggttggat gttgcgtaag ttcgtcttat tggttatagt tggggtttcg
      121 caatgttcgc gttaccccca gccgaattac ctggcgctgt tgcttgcttc agcgtctacg
      181 gtttcgcaaa atccagttaa gtcggtgtat tccttcgtaa cgaacgtagc aaccaatccc
      241 actctgatga ccgtaccggc cgtagatacg gcaagagctt ttgtaatgtg ttcttatcgc
      301 cttgcaaata cgagctttca aacgaaccgg gttccgattt gttctctcac ctcctcgacg
      361 caggtaaccg ttcaggcagg gacctcagat gccggggttc tggttcgagg ttacgtggtt
      421 gagttttcta ccggcgtgaa tgtacagcgc ggtgctttca ctctggccgg aggcacactg
      481 aacaacactg tctcaatttc tcccgtaagt cagaatcgtt catttgtgat tgtgcagtca
      541 aggaccacgg acactggaac actcattgat gaacaccgga ccgtaatggg gtatctacag
      601 agttcaagta gtctctacgt ggttcgtaat caagcaacca tcattacgga cgttgaatat
      661 caggtgatcg aaatgcaagg cgcgacggtg cagtatggca tcagccagat accaggagga
      721 tcgttcagtg tgtcgattcc gatcgctgcg gtgaatacct cgaagtcttt cgttgttttt
      781 agttctgcgg cagacaacct ttcgaatgga aatgacagtg agatggcagt gaggtgtgac
      841 cttgccaatg cttctcagtt gctatgtcag agacaggatt ccacgaatgc cccagatatc
      901 gcctggtatg ttgtttcttt tgcggacagc acatatgtcg aacgaggaag cgctgatacg
      961 aatgtattca ctccgccggc aaacaacacg gacacgagca tgactgtgac tccctctgtc
     1021 ccattcgatc tgaccaggac gttgccgatt ggatccaccg cttcagatcc aggagatgcg
     1081 gacgcgggtc tggattccgc gtcattcact cagcatctac ttacttcaac gcagctaaac
     1141 attcaaagag gatcggcaga cgatcggcgt agcgttgtat cctggcaact gatccagttt
     1201 cctgcgcgtt agcatcacct tctcattgtt aggtgctgcg cgtatgggct agtgccagaa
     1261 gtcagcagga atggtcgcgg ctgaggaagc caggcgaacg agtttgtcca ctcgcagtgc
     1321 cgtcaggttg cttttgaaat cgtccgtcac tccgcacaga atgacttttc ctttcttctt
     1381 ttccaaatca cgtgcgaggc gaattaaaaa accaatgcct gaagaatcca ccatagggac
     1441 tttgcttaaa tccacggcga gtttcatccc atccgttgtt ttctttatga tcgcatttgt
     1501 aagttgcccg gccgaataga tgttgatggg cccggaaata tttactcgaa atcctttttc
     1561 aaaaggaaga atttctgaaa tctcaagttc tgcagaattg ctcattgaac cccccttttc
     1621 tgcaccagga tcgcttcgat ggacgtcagc ggcatgctgt ctcctccaac cgcgaattcc
     1681 tggttcttgc cttcgatcgc ttcgccacag agtacgtgcc gtgagcgatc aatagcgcac
     1741 acgttgtaat cggtacgcga aggcgcaagt cgaattgacg cgatgaggat cgccgggacc
     1801 gcgacgggac gcgaacctgc gagaatcgcg cgatcgtctt ccatgagaat agagaaaccc
     1861 tgaagggttc gtccatcgga caacacgacg ctgattccag gagggggctc ttcgcgcgca
     1921 ggggtcgctt ctatcaaggc ttcgcgcttt tcaatctcag tcttgatctc ggatacaaat
     1981 ttcacttcgg gagcctcctt cgctgaaact tt
//
LOCUS       DAOQJL010000652         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_45547_length_2011_cov_4.900307, whole genome shotgun sequence.
ACCESSION   DAOQJL010000652 DAOQJL010000000
VERSION     DAOQJL010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45547_length_2011_cov_4.900307"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..759)
                     /locus_tag="PLG78_19240"
     CDS             complement(37..759)
                     /locus_tag="PLG78_19240"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE25318.1"
                     /translation="MARVQIIGSGNAFNMDGRAHACYLIESDLLLDCGASSVLRMQSL
                     HVDLSFIAGVLFTHFHGDHLAGLPFLLLNYKYILKRSESLTILGPRGIQETCERLLEL
                     TYPGMTFEFPIHYFEIIPGRDVAFRDKTIRGMPVTHRPESLGYRITWEKDSSRRTFAF
                     SGDAAYDSQLFELVRGVDLAVVELSLVENDGSVAHVALSEVRDGSLRSQRVVYSHVYD
                     ELANLARMEGLTVAEDGMILEF"
     gene            complement(762..>2011)
                     /locus_tag="PLG78_19245"
     CDS             complement(762..>2011)
                     /locus_tag="PLG78_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25319.1"
                     /translation="LGGKYHVELISHAAMLPTDLLNYRKRIAALKPAAVVWTTNPVDL
                     DLERLTPIWEAGPYRSQRTERNFVSRRIPARLYYPDSIMRESEIGAELTSAYAVRTAL
                     YSARYPRDEWLPFVQFVSDWNGPLKSYLNYQGIPIAGGIFREGFTGACFGLSEEVTGK
                     EIWMEFPSALYSAGVRLVVRPLRAAEMATCDSFSGTEIVPSRAGWQQITLPPGKLEIR
                     LTHVMVEGSVKEMQSGPAFQGRGVRLPGNLGRREPATQEVYLRRPNLEEARFESMSER
                     ELILDYENRMNPPDWRLRPPLVNMGTIRLSKTLVAYAGFVETRQMADMKHFITELAAD
                     VPVLIVNQAEHPLSLDEYVWSQWYADYVGFIQAMKSSRVSVLDLHDALPMRELGDPHH
                     LTFKGARHVQPMMADALSKLLGK"
BASE COUNT          522 a          543 c          491 g          455 t
ORIGIN      
        1 cgaatacgtc accgctcgct agtgaaactt cgaaaatcaa aactccagaa tcattccgtc
       61 ctctgccacg gttagacctt ccattctggc aaggttcgcg agttcatcgt aaacatgaga
      121 atagacaacg cgctggctgc gaagagaccc gtcccgcact tcgctcagtg caacatgcgc
      181 cacacttccg tcattttcca ccaggctcaa ttctacaact gcgagatcta ctccgcgcac
      241 aagttcgaat agttgtgaat cgtaagccgc gtcgccagaa aaagcaaagg tccgacggga
      301 ggaatccttt tcccacgtta tgcgatatcc gagactctcc ggtctatgag tcacgggcat
      361 cccgcgaatg gttttgtccc tgaatgctac atctctaccg gggataattt caaaatagtg
      421 gatgggaaac tcgaaagtca ttccaggata agtcaactcg agtaaccttt cgcacgtttc
      481 ctggattcca cgcggaccca gaatggtaag gctctctgat ctctttagaa tgtatttgta
      541 gttcaatagc aagaacggaa gtccagcaag atgatcgccg tggaaatgag tgaacagcac
      601 tcctgcaata aaagatagat ccacgtgcag agactgcatg cgaagaacag aagaagcgcc
      661 acagtccaaa agaagatccg attctatcaa atagcaggca tgggcccgac catccatgtt
      721 gaacgcattg ccggatccaa tgatttgaac ccgggccatt cctattttcc caggagtttc
      781 gaaagagcgt ccgccatcat gggttggacg tgacgagcac ctttaaacgt aagatggtgc
      841 ggatccccga gttcgcgcat gggaagagca tcgtgcaaat cgaggacaga cacgcggcta
      901 gacttcattg cctgaatgaa gccaacgtaa tccgcatacc actggctcca gacgtattca
      961 tcgagcgaaa gtggatgctc tgcctgattg acaatgagaa ctggaacatc cgcggccagt
     1021 tccgtaatga aatgcttcat gtccgccatc tgccgagttt ccacaaagcc ggcgtatgca
     1081 accagagtct tggataagcg aatcgtgccc atattcacaa gaggtggacg caacctccaa
     1141 tcgggaggat tcattcgatt ttcgtaatca aggatgagtt cacgttcgct catggattca
     1201 aagcgagctt cttccaggtt gggtcttcgc agatatactt cctgagtggc aggctcacgc
     1261 cgcccaagat tccctggaag gcgaacaccc cttccctgga aagccgggcc tgactgcatc
     1321 tcttttacac ttccttcgac catcacgtga gtcagtcgaa tctcaagttt gcctggaggc
     1381 agagtgattt gctgccaacc agcgcgagac ggaacaatct ctgttccaga aaaagaatca
     1441 cacgtcgcca tttctgcggc gcgcagtgga cgcactacaa gcctcactcc tgcagaatag
     1501 agagcggacg gaaactccat ccagatttct tttccagtca cttcttcgct cagaccaaag
     1561 caggcgccag tgaatccttc tctgaagatc ccacctgcaa tggggattcc ctgatagttg
     1621 agataactct tgagcggtcc gttccaatca gatacaaatt gaacaaaagg cagccattcg
     1681 tcgcgcggat agcgagcgga ataaagcgca gtcctcaccg catacgcact cgttagctct
     1741 gcgccaatct ccgattcgcg catgatgctg tcaggatagt agagcctggc gggaatccga
     1801 cgcgaaacaa agtttctctc tgtacgctgc gacctgtatg gccctgcttc ccagatcgga
     1861 gttaaacgct ctagatccag atccactgga ttcgtggtcc agacgacggc ggccggtttg
     1921 agtgctgcaa tgcgtttgcg ataattcagc aggtctgtgg ggagcatggc tgcatgcgaa
     1981 atgagctcca catgatattt gcctcccagg t
//
LOCUS       DAOQJL010000653         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_45960_length_2002_cov_3.327170, whole genome shotgun sequence.
ACCESSION   DAOQJL010000653 DAOQJL010000000
VERSION     DAOQJL010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45960_length_2002_cov_3.327170"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /gene="gatA"
                     /locus_tag="PLG78_19250"
     CDS             complement(<1..1392)
                     /gene="gatA"
                     /locus_tag="PLG78_19250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNE25320.1"
                     /translation="MDIRKTAFAHAESLQRGEYTALELAQSCLAEAKRRSSLNIFLAL
                     DEERVLSQARESDARRKNGKSLGILDGIPVAIKDNICAKGEKTTCASKILENFEAPYD
                     AHVIERLRANGAVLFGRTNLDEFAMGSSTENSAFMITKNPWDESRVPGGSSGGSAAAV
                     GAGIVPLALGSDTGGSIRQPASFCGIHGLKPTYGAVSRYGLVAFASSLDQIGPFARSA
                     QDASLLLAAIAGRDLRDSTSSADSSKVVAPFGGAHSDAEIRKLRIGYYLPEKKDGYAA
                     EVLAAVQKSVDYFASKGAKMVPVKSEYWEYSIPIYYILATAEASSNLSRFDGIRYGHR
                     AKGEELKEIYIRSRTEGFGPEVKRRILLGTFVLSAGYFDAYYGKAQKAKALVRAEYID
                     FFKNLDFILQPTSPTTAFAIGEKAKNPIAMYQSDILTIAANLGGVPAMSVPAGRDGKG
                     LPIGIQITGAHFSD"
     gene            complement(1398..1682)
                     /gene="gatC"
                     /locus_tag="PLG78_19255"
     CDS             complement(1398..1682)
                     /gene="gatC"
                     /locus_tag="PLG78_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNE25321.1"
                     /translation="MLSEEQFSSLAFLARLDPEDAGLKGLRMDFNKILDAVKTIEELD
                     LHGAEGTSVPGPAVTRPDEPVGELSPREISKFAPEWEAGHFVVPGVIQSE"
     gene            1789..>2002
                     /locus_tag="PLG78_19260"
     CDS             1789..>2002
                     /locus_tag="PLG78_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25322.1"
                     /translation="MEHATTDGTGRDPTLLEDIRIIFATILTDPGSSLAYGADALIHV
                     TIGLIVSSYTLGVQATFIAAGIVLGVY"
BASE COUNT          473 a          539 c          497 g          493 t
ORIGIN      
        1 atcgctgaaa tgggctcctg tgatctgaat gccaatgggc aatcctttgc catcccttcc
       61 cgccggcaca ctcatcgccg gcacaccgcc cagattcgct gcaatggtga ggatatccga
      121 ttgatacata gcgatcgggt tctttgcttt ttcgccgatt gcgaaggcag tcgtaggaga
      181 cgttggctga agaatgaaat cgaggttctt aaagaaatct atatattctg cgcgcacgag
      241 agcttttgct ttttgcgctt tgccgtagta tgcgtcaaag tacccggcgg ataaaacaaa
      301 cgttccaagc agaatgcgtc ttttgacttc aggaccgaac ccttccgttc ttgatcgaat
      361 atagatctct ttgagctctt cccctttcgc gcggtgtccg tatctgattc catcgaagcg
      421 actcagattg ctggaagctt ctgcagttgc gaggatatag tagataggaa ttgaatattc
      481 ccagtactct gatttgacgg ggaccatctt cgcgcccttc gaagcaaaat agtctacgga
      541 tttctgaacc gcggcgagaa cttccgcggc atagccgtcc ttcttctccg gcaggtaata
      601 tccaattctg agtttacgaa tttcagcgtc tgaatgcgcg ccaccaaacg gtgcaactac
      661 tttagaagaa tccgcgcttg atgtagaatc tcgcagatca cgcccggcga tggccgcaag
      721 caacaaggat gcatcctgcg cgctgcgagc gaaaggtccg atctggtcaa gactggatgc
      781 aaacgcaacc aggccgtatc gcgaaacggc cccgtatgtg ggtttgaggc cgtgaatgcc
      841 acagaaagaa gcaggctgtc gaatcgatcc gcctgtgtct gagccgagcg caagaggcac
      901 aatgcccgca cctactgctg cggcagatcc gccagaggat ccgccaggaa cgcgagattc
      961 atcccacgga ttttttgtaa tcatgaaggc agagttttct gtcgaggaac ccatggcgaa
     1021 ttcgtcaaga ttggttctac caaaaagaac tgctccattc gcacgaagac gttcaatcac
     1081 atgcgcatcg taaggcgctt caaaattctc aagaattttg gaggcgcaag ttgtcttttc
     1141 ccctttcgcg caaatattat ctttgatcgc aaccggaatg ccgtcgagaa tacccagtga
     1201 ttttccattc ttgcgtcgcg catctgactc gcgggcctgc gaaagcaccc tctcttcatc
     1261 gagagcgaga aaaatattca agctggatct tctttttgct tctgccagac aactttgtgc
     1321 gagctcaagc gccgtgtatt caccgcgctg cagagattct gcgtgagcga acgccgtttt
     1381 tctaatatcc attatgatta ctccgactga atcacgccgg gtacaacgaa atgacccgct
     1441 tcccactcag gggcaaattt tgaaatttcg cgcggcgaaa gctcgccgac cggttcatcc
     1501 ggcctcgtaa cagcgggacc gggaacagat gtcccttctg cgccgtgcag atcaagctcc
     1561 tcaatcgtct tgactgcatc aagaatcttg ttgaaatcca tgcgaaggcc tttgagaccc
     1621 gcatcttcag gatcgagtct ggcaaggaac gcaaggctgc tgaattgttc ttccgagagc
     1681 atattcctct acgcatcagg tgagcgatgt gtcaaggaaa acactgccgc gagaaatcaa
     1741 cgatttcact tgcgttgcca catgcctcgc gtagccttgc agtgttctat ggaacatgca
     1801 accacagatg ggaccggccg cgatcccact ctccttgaag acattcgcat catctttgcc
     1861 acgattctga cagacccggg aagctcactg gcttacggcg cagatgccct cattcatgtt
     1921 acgatcggat tgattgtttc gagctacact ctgggagtcc aggcgacgtt cattgcggcc
     1981 ggcatcgtgc tcggagtgta tt
//
LOCUS       DAOQJL010000654         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46177_length_1997_cov_3.613800, whole genome shotgun sequence.
ACCESSION   DAOQJL010000654 DAOQJL010000000
VERSION     DAOQJL010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46177_length_1997_cov_3.613800"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLG78_19265"
     CDS             complement(<1..455)
                     /locus_tag="PLG78_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25323.1"
                     /translation="MSFFQPRNIWLVIMVAAIGVSMYVREESKTPDIEVLKTKQISMM
                     VKMLASQALVVAKVPGPLTDGVKKQIKDQLGDIETKLPEREIFPVQKFIILSEIGESV
                     SLPDMGNKALIADLKLIYVDRTPAADNSPLFAIAGGDLARLKSLSLNKP"
     gene            complement(452..1213)
                     /locus_tag="PLG78_19270"
     CDS             complement(452..1213)
                     /locus_tag="PLG78_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25324.1"
                     /translation="MILILAGAGARFGTGHVERMRILKGLLEARGHSVSLRLTDEPEA
                     ERGRHGDSLIVFDARDSACDVGERVIAVDNRSSIRAGNTSIVFHDTLPHPEVAMDVAL
                     NQCLMDPALLTLERRTEDKASILVYSGQEAPPELAVEGKVFQVGRDRMLGAIGANRLD
                     RTAFLERLAASRVLVSYFGMTVLEAMFLGIPTVTYSIGSTVHDTLSALLEKETNVRFY
                     RKSQKAGQEIFDLTYTKRPSGQGFQILIDLIERYL"
     gene            complement(1210..1938)
                     /locus_tag="PLG78_19275"
     CDS             complement(1210..1938)
                     /locus_tag="PLG78_19275"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25325.1"
                     /translation="MKCAVLIQARSASVRLPGKIYEPVPESGSPSILDHVLRRMQLAN
                     VGPVVVLIPEDDTLLADYLSVRRIEFLRGPHEDVRARYRHAARAMNLDLVVRATADNP
                     ALDPVCAARSVQEIQSRNLDLFSFSAMPLGTGVEVFTAAALLSDSLEGPEYSEHVSLH
                     IKHAPDRFRVEHSPWREGLPPELPRLTVDTREDLEVVRSVFRKLGVDFTLDQLMELFA
                     SNPELFAGNRHVVQKTFLPPGASS"
BASE COUNT          517 a          545 c          473 g          462 t
ORIGIN      
        1 tttggtttat tcaaagacaa agatttcaaa cgagcaagat ccccgccagc aatggcaaat
       61 aaaggcgaat tgtctgccgc aggagtgcga tccacgtaaa tcaatttcaa atcagcgatg
      121 agtgctttgt ttcccatatc cggcagcgac acgctctctc ctatttcaga aagaatgatg
      181 aacttttgca ccggaaaaat ttctctttct ggcagtttgg tctcaatgtc gcctaactgg
      241 tctttgattt gtttcttgac tccgtccgtc aatggcccgg gcactttagc cacaacaaga
      301 gcctgagatg caagcatctt gaccatcata ctgatctgtt ttgttttgag aacttcaatg
      361 tccggggttt tagactcctc ccgaacatac atcgacacac caatggccgc caccataatc
      421 acaagccaga tgttacgcgg ttgaaaaaag ctcataaata cctctctatc aaatcaatca
      481 aaatctggaa tccttgcccc gatggacgct tcgtatatgt tagatcaaaa atttcctgac
      541 ccgctttctg gctctttcga tagaagcgga cgtttgtttc tttttcgaga agagcagata
      601 acgtatcatg cacagtgctt ccaattgaat acgtaactgt gggaattcca agaaacatag
      661 cctcgagaac agtcattcca aagtaagata caagtactcg cgaagcagcc aatcgctcta
      721 gaaacgcagt gcgatccagc ctgttagctc ctatagcacc gagcattcta tcgcgtccca
      781 cttgaaaaac cttcccttcc actgcaagtt ccggcggcgc ttcctggcca ctgtatacga
      841 gaatgctggc tttgtcttcc gtccgtcttt ccagcgtcag aagtgcagga tccatcaaac
      901 actgattgag cgcaacgtcc atagccacct caggatgagg cagagtgtca tgaaacacaa
      961 tgctcgtgtt gcctgcgcga atagaggaac gattgtccac tgcgatgacg cgttccccca
     1021 catcgcaggc agaatcgcgc gcgtcaaaaa caatgagcga atctccatgt ctgccacgct
     1081 ccgcttctgg ctcatctgtg aggcgaagag acacagaatg accgcgggct tcgagaagac
     1141 ctttaagaat tcgcattctc tctacgtggc ccgtcccaaa tcgggcgccc gcacctgcca
     1201 gtatcaagat caagacgatg ctccaggcgg cagaaatgtc ttctgaacca catgacgatt
     1261 gcccgcgaat agttcgggat tggatgcaaa tagttccatg agttgatcga gagtaaagtc
     1321 cacacccagc ttgcgaaaca cggatcgaac cacttccaga tcctctcgtg tatccacggt
     1381 taggcgggga agttccggag gcagcccctc tctccatggc gagtgctcca cgcgaaaccg
     1441 gtctggggca tgcttgatgt gcaaagatac atgttcactg tactcgggtc cttccagaga
     1501 atcagacaag agggcggcag ccgtgaagac ttccactcct gtgcccagag gcatggcaga
     1561 aaaagaaaaa agatccagat tgcgactctg aatttcctga acggaacgcg ccgcacagac
     1621 cggatcgaga gccggattgt ctgcggtggc gcgcacaact aaatccagat tcatggctcg
     1681 cgccgcatgg cgatatcggg cgcgaacgtc ctcatgcggg ccccgaagaa actcaatccg
     1741 ccgcacgctt agatagtcag ccagaagcgt atcatcttct ggaatgagca ccaccaccgg
     1801 gcccacgttt gcaagttgca tacggcgcaa cacatgatcc agaatagatg gtgaaccgga
     1861 ttctggcacg ggctcgtata tttttccggg aagcctgacg gatgcgcttc ttgcctggat
     1921 gaggactgcg cacttcatcc gttgaacgtg taccactcgt cacagttcag gcagggcatt
     1981 cctacagaat cgtgctc
//
LOCUS       DAOQJL010000655         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46253_length_1995_cov_4.528351, whole genome shotgun sequence.
ACCESSION   DAOQJL010000655 DAOQJL010000000
VERSION     DAOQJL010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46253_length_1995_cov_4.528351"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLG78_19280"
     CDS             <1..348
                     /locus_tag="PLG78_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25326.1"
                     /translation="LLGGAIGAGTFRFLESRVPEILALFRKPEMPQAAELEYDNESPG
                     FESPLSAGDSGGSVDESDSPPQVSTSTGSMPTPSAAPAAYGDHILVNKVKIKNEPKLM
                     AAAIRTLLAKDEK"
     gene            400..1197
                     /gene="whiG"
                     /locus_tag="PLG78_19285"
     CDS             400..1197
                     /gene="whiG"
                     /locus_tag="PLG78_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HNE25327.1"
                     /translation="MSRLWDKYKDKDETELWRRYRKEANREIRDFLAEKYAPLVKYVA
                     GKVAIGMPQNVEFDDLVSYGSFGLLDAIEKFDPDRDIKFKTYAMTRIRGAIFDELRSI
                     DWIPRSIRQKARQVEETIAMLENKLGHTVEDEDIAKELGISVDELQSLLTKISGTSIV
                     SLNDIWYLGDDNDEVSFMETLESPANLNPDSLIEREEIKSVIVEAIRHLPDKEKKVIV
                     LYYYEDLTLKEIGEVLEVTESRISQLHTKAIMRLRGKLMRMKNALKK"
     gene            1314..1664
                     /locus_tag="PLG78_19290"
     CDS             1314..1664
                     /locus_tag="PLG78_19290"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNE25328.1"
                     /translation="MDAISIVGHSPTPTRPHGDQTAVRQNEAAAVAAQQTEATEPNVH
                     KIASAVQALNLFHEDKEHTRFQYRVNPEYGTVQVTLFNYMTGEIISEIPSRKVLDFAQ
                     RMREISGMLLEKQA"
     gene            complement(1909..1995)
                     /locus_tag="PLG78_19295"
                     /pseudo
     CDS             complement(1909..1995)
                     /locus_tag="PLG78_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
BASE COUNT          561 a          495 c          485 g          454 t
ORIGIN      
        1 cttcttggcg gggccatagg tgccgggacg tttcgatttc tagaatcccg ggttcctgaa
       61 attctggccc tgtttcgcaa gcctgaaatg ccccaggccg cagaattaga atatgacaat
      121 gaatcaccgg ggtttgagtc gccgctcagt gcaggcgatt ctggcggatc cgttgacgaa
      181 agcgattcac ctccgcaagt atccacgtcc acaggtagca tgcctactcc gtcggcagct
      241 cctgcggcat acggcgatca cattctggtc aacaaagtga agatcaagaa tgaaccgaag
      301 ctaatggcgg cagcaatccg cacacttctt gcgaaggatg aaaagtaaaa acagtgtttt
      361 gcttgctaac ccacaaaaca cactgcatgg tgacgtaata tgtccagact ctgggataaa
      421 tacaaggaca aagacgaaac cgagctctgg cgtcgttatc gcaaagaagc aaaccgtgag
      481 atccgcgatt tcctcgcgga aaagtatgct ccccttgtga aatacgttgc gggcaaagtc
      541 gccattggca tgccgcaaaa cgttgagttc gatgatctgg taagctatgg ttctttcgga
      601 ctcctggatg caattgaaaa gttcgacccg gatcgtgaca tcaaattcaa aacgtatgcg
      661 atgacgcgta ttcgcggagc catcttcgat gagcttcgca gcattgactg gattcctcgt
      721 tctattcgtc agaaagctcg ccaggtagaa gaaacaatcg caatgctcga aaacaaactc
      781 gggcacacag tagaagacga agatattgca aaagagctgg gcatttcagt agatgagctt
      841 cagtctcttc tcacaaagat ctcaggcaca agcattgtat ccctcaacga tatctggtat
      901 cttggagacg ataacgacga agtctccttc atggagaccc ttgaatctcc cgcaaacctg
      961 aaccctgatt cactcataga aagagaggaa atcaaaagcg taatcgtgga agccatccgt
     1021 catttgccgg acaaagaaaa gaaagttata gttctttact attatgaaga cctcaccctg
     1081 aaagaaatag gggaagtcct tgaagtcaca gaaagccgaa tttctcagct ccacaccaaa
     1141 gcaatcatgc gtctgcgcgg taagctcatg cgcatgaaaa atgccctgaa aaagtgactt
     1201 ccctcggatc ttctgaccgg aaactttaat cttttttctt aacgtactgt taaggtactt
     1261 catccagtta ccgatttgca atctagggac agggatgtcc caggagatcg agaatggatg
     1321 ctatcagcat agtcgggcat agccccacac ccacacgacc tcacggggat cagacagccg
     1381 tgagacagaa tgaagctgct gcggttgcgg cgcagcaaac cgaggcaaca gagcctaacg
     1441 ttcacaaaat tgcttctgcg gtgcaggcac tcaacctgtt ccatgaggac aaagagcaca
     1501 ccaggtttca atacagggtg aatcctgagt atggcacagt tcaggtgacg ctgttcaatt
     1561 acatgaccgg cgaaatcata tccgaaattc catccagaaa ggttctggat ttcgcacaga
     1621 gaatgcgtga gatttcggga atgctacttg aaaagcaggc ctgaaaggcc tgcttgtttg
     1681 ccttaagccg gctagagccg gcgattccac agcagcttac ttgctgtaga actcaacgat
     1741 cttctgtacg ttgatgcgcg taaccggaat atcggttaca ctttgaattc ctagaaactt
     1801 gccagataca ccgtcagact gcacctccag gtatggagtg ggcgaactca cagatgcacg
     1861 tgtttccaga gcgcttttca ctcgatccag ttccgtagag ccgggagtca gtccaatgat
     1921 ctgaccaggc gagacactga agcttggtac gtctacacgt tgtccgtcta cagatacatg
     1981 cccgtggcga atgag
//
LOCUS       DAOQJL010000656         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46647_length_1987_cov_2.722567, whole genome shotgun sequence.
ACCESSION   DAOQJL010000656 DAOQJL010000000
VERSION     DAOQJL010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46647_length_1987_cov_2.722567"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLG78_19300"
     CDS             <1..979
                     /locus_tag="PLG78_19300"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNE25329.1"
                     /translation="EDGDHGMAQFLLQTSYRQPQAAGFPDIPSMGAVFSHLKSKKVEA
                     GYFPSHVTMGERGGMTGAAGFLGARNSGFHISNLDNAISYMKPAWGNFGAERLVRRGQ
                     MLDMLNDRFRKKYSSAGLDLWQQMSEAALEFVNSSRLSVFDLKQERADVRARFGDNWF
                     GKACLMAKRLASAEVPFIQITRGGWDTHDNNRGRVTEIMKDVDPALAALLGELGSSGL
                     LKQTLVVLTSEFGRTPNVGGRDGRDHWPQAWTTLIAGGNVPGGAVIGDTGEKGEKPVK
                     DGVHWRGAAATIYRAGGIDPEAMLKNEMGRPLRLVAQGNNEPVRALVGE"
     gene            complement(991..>1987)
                     /locus_tag="PLG78_19305"
     CDS             complement(991..>1987)
                     /locus_tag="PLG78_19305"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNE25330.1"
                     /translation="ERTGEYTFYTQTDDGVRLTVDGKMLVDRWQDMGTTEFSGKINLQ
                     SGRTYDFKMEYYERGGGAEARLLWSGPGTPKQVLRPIGAVAQTGPAADLEDKPLRLGV
                     MDVNADRRADVLVLSSGFPGKITTLLAKQDGFFEEGGSVSAGNDPGILVVSEGRAAVL
                     ARQSRQIIVFKPVQGKLTQVNARKLDQKLRGLENLENGFAVLGEKSVLILGEDLADRS
                     TISAEGAIALATGDLDGDGRADIAVLSSSGATAYYAKDNYQKSELFPGTKPGSDQATQ
                     ALIYQRKIYSSTPAGIFQMDREGALNRSDAPGRIVRGESGLFVLETGRGQIRPMP"
BASE COUNT          437 a          510 c          543 g          497 t
ORIGIN      
        1 cgaagatggg gatcatggta tggcgcaatt tctgctccag acttcttaca ggcagcccca
       61 ggcagcgggg tttcccgaca ttccgtccat gggcgcggtc ttctctcatc tcaaaagcaa
      121 aaaggtcgaa gcaggttatt tcccgtcgca tgtcacgatg ggagaaagag gcgggatgac
      181 gggggctgcc gggtttctgg gtgcgcgaaa cagcggattt catatttcca atctggacaa
      241 cgccatcagt tacatgaagc ccgcgtgggg aaattttggt gcggaacgtc tggtcagacg
      301 ggggcaaatg ctcgacatgc tcaacgatcg ctttcgcaag aagtattcat ctgccggtct
      361 ggatctgtgg cagcaaatgt cagaagcggc gcttgaattt gtaaactctt ccagactttc
      421 cgtttttgat ctcaagcagg agagagcgga tgttcgcgct cgttttggtg acaactggtt
      481 tggcaaggca tgcctcatgg ctaagagact tgcttccgcg gaagttccct tcattcaaat
      541 cacacgcgga ggatgggata cccatgacaa caaccgcggg cgcgtcacag aaatcatgaa
      601 ggatgtggat cctgctctcg cagcactcct gggtgagctt ggaagttccg gtcttttgaa
      661 gcaaactctg gttgtactga cgagcgaatt cggtcgcact ccgaatgtgg gcgggagaga
      721 tggaagggat cactggccgc aggcgtggac tactctgatt gctggaggaa atgtacctgg
      781 cggagcagtc atcggagaca ctggagaaaa aggagaaaag ccagtgaaag atggcgtaca
      841 ctggagagga gcggccgcca ccatctatcg cgcaggtgga attgatccag aagcaatgct
      901 caagaatgaa atgggccgtc ccctcagact cgttgcgcag ggaaacaacg agccggtgcg
      961 tgccctcgtg ggcgaataac gttctctggt ttaaggcatg ggccgaattt gaccgcggcc
     1021 cgtttccagt acgaaaagtc cggattcacc gcgaacgatt ctgcctggcg catcggatcg
     1081 attgagcgct ccttcgcgat ccatctggaa gattccggct ggagtggagg agtaaatctt
     1141 tctctggtaa atcagagcct gagtcgcctg gtctgaacct ggttttgttc ctggaaatag
     1201 ttcagatttt tgatagttat cttttgcgta gtaagccgtg gctccgcttg aactgagcac
     1261 tgcgatgtct gccctgccat ctccatccag gtctcccgtt gccagggcta ttgcaccttc
     1321 tgcggaaatt gtggatcgat ctgcaagatc ctctcccaga atcaggactg atttttcgcc
     1381 taacactgcg aagccgttct ctagattttc cagtccgcga agcttctgat ccagctttct
     1441 tgcgtttacc tgggtgagct ttccctggac tggcttaaag acaatgattt gcctggactg
     1501 acgggcaaga acggccgcgc gaccttccga aactacaagg attccaggat catttcccgc
     1561 gctcacactt ccaccttctt caaaaaagcc gtcctgtttt gccagtagag tagtgatttt
     1621 tcccggaaat cctgaggaaa gaacgagcac atccgcgcgg cgatccgcat ttacatccat
     1681 gacgccaagt ctaagtggct tgtcctctag atctgctgcc ggtccagtct gagctactgc
     1741 tccgatgggt cttagaacct gtttgggtgt tcccgggcca gaccagagca atcttgcttc
     1801 tgctcctccg cctcgttcgt agtattccat tttgaaatcg taggttctgc ccgattgaag
     1861 attgattttc ccggaaaact cagtggtgcc catgtcctgc cagcgatcga cgagcatctt
     1921 tccgtctacc gtcaggcgca caccgtcatc tgtttgagtg tagaatgtat attctcctgt
     1981 tctctct
//
LOCUS       DAOQJL010000657         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46715_length_1985_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOQJL010000657 DAOQJL010000000
VERSION     DAOQJL010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46715_length_1985_cov_4.000000"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PLG78_19310"
     CDS             <1..1169
                     /locus_tag="PLG78_19310"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE25331.1"
                     /translation="WEAEIGGDLATANVITLRGRRYAVFINGDAMGKSIQGAGGALVL
                     GTVFKSILSRTQVSSYFQNRYPEQWLKEMFLELQSVFTAFDGSMMVSAVFGLVSEESG
                     LVYYVNAEHPFVVLYRRGKASFLDRSMELRKLGIAIIPGILQVRSVPLRPRDVLIIGS
                     DGRDDVAIGEDSLGNRIINEDESHFLRSVEKGDGNLERIEEAIRETGELTDDLSLMRI
                     AFRDDVPLTEESSVDQARDRIAVARAHARSGRLRQAEEDYRAAAESSPGPAIGREWLK
                     VLHAMGRESERAEIAERYSELHPEVPGFLRVASEALAACGNIEKAVDFGERYRLCQPY
                     NRAGLLHLVDLHMRQGRWTAALRVVEQRLIVDPMDREALELRLLLRQKMGSDDA"
     gene            complement(1181..1558)
                     /locus_tag="PLG78_19315"
     CDS             complement(1181..1558)
                     /locus_tag="PLG78_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25332.1"
                     /translation="MSVEQVISELRQSGRCIDKPLFSAGDLQTAEQGLGIELPLSYKI
                     LLTTLEPEMTNFYFQPPARHDSFQNLIVFATWDEDQFAFDTERKMRVITLLGTSMRGR
                     EWEDFDGWLAHTWSMSNRPASTE"
     gene            1659..>1985
                     /locus_tag="PLG78_19320"
     CDS             1659..>1985
                     /locus_tag="PLG78_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25333.1"
                     /translation="MKYSLFLLWILVAATPAFAEECFATPSLYSYTFPKSHLKQYPKQ
                     TVAEMDLSWQDEQTCPHARASISIKLWSGVSYRFFADCAPLGQGRYKCAKGASAASLR
                     FDGKFAW"
BASE COUNT          436 a          460 c          575 g          514 t
ORIGIN      
        1 actgggaagc ggaaatcggc ggagatctgg ccacggccaa tgtaatcacc cttcgcggaa
       61 gacgttatgc ggtattcata aacggggacg ccatgggcaa atcgattcag ggtgccggcg
      121 gggctctcgt gctcggtacg gttttcaagt ccattctctc tagaacccag gtttcgtcat
      181 attttcagaa tcgatatccg gagcagtggc tcaaagagat gtttctcgag ttgcagagtg
      241 ttttcactgc ctttgatggc agtatgatgg tatcggctgt gttcggtctg gtgtcggagg
      301 aaagcggcct tgtgtactac gtcaacgcgg agcatccgtt tgttgtgttg tatcgtcgcg
      361 ggaaggcgag ctttctcgat cgctctatgg agttgcgaaa gctcggcatt gcgatcattc
      421 ctgggatttt gcaggtaagg tccgttcccc tgcgtccgcg cgatgtgttg atcatcggct
      481 ctgacggacg cgatgatgtt gccatcggag aggactcact tggcaatcga atcatcaatg
      541 aggatgaatc gcactttttg agaagcgtag agaaaggaga tggcaatctg gaacggatag
      601 aagaggccat tcgggaaacc ggagaactga cggatgatct ttccctaatg cgaattgcat
      661 ttcgagatga tgttcctttg actgaggaat cgtctgtaga tcaggcccgt gatcgtattg
      721 cggtggcccg ggctcatgcg agatcaggaa ggctcaggca ggcggaggag gactacagag
      781 ctgcagccga aagttcgccc gggcctgcca tcggacgcga atggctcaag gttcttcatg
      841 cgatgggccg cgagtcagaa agagcggaga ttgcggaacg ttattcggaa ttgcacccgg
      901 aagttcctgg atttttgcgc gttgcctctg aagccctggc cgcctgtggc aatatcgaaa
      961 aagcggtgga ttttggagaa aggtacaggc tttgtcagcc atacaatcga gcgggtctac
     1021 ttcatctggt cgatttgcac atgaggcagg gcaggtggac ggcagcgcta cgagttgtgg
     1081 agcagaggct cattgtagac cccatggaca gagaagccct cgagttgcgt cttctgctcc
     1141 gtcaaaaaat gggaagtgac gacgcgtaaa tcgagagctg ttattctgta ctggcagggc
     1201 ggttactcat ggaccaggta tgtgcaagcc agccatcaaa atcttcccat tcgcgtccac
     1261 gcatgctggt cccaagaaga gtgatcacgc gcatttttct ttccgtatca aaagcaaact
     1321 gatcttcatc ccatgttgcg aacacgatta gattctggaa gctgtcatgg cgcgctggag
     1381 gttggaagta gaagttcgtc atttctggtt cgagagtagt cagcaggatt ttgtaggaaa
     1441 ggggtagctc aattccaagt ccctgttctg cggtttgaag atcccccgct gaaaaaagag
     1501 gtttgtctat gcatctgccc gattgtcgca gttcggagat aacttgttct acgctcatgg
     1561 ggacatgctg catgagcgcg ttccggggat caatgatttg tcgacgcaaa tcattgaatt
     1621 ctccttgcct gccggcagct ctgtgtttcc tattcgttgt gaaatattct ctttttctct
     1681 tatggattct cgtggctgca accccggcct tcgcggagga gtgttttgcg actccgtctc
     1741 tttactctta tacttttcca aaatcgcatc taaagcagta cccaaagcag acggtcgcgg
     1801 agatggatct ttcctggcag gatgaacaaa cgtgccccca cgctcgcgca tccatttcta
     1861 tcaaattgtg gtcaggcgtt tcctatagat tttttgccga ttgcgcgccg ctcgggcagg
     1921 gcagatataa atgtgcgaag ggcgcgagcg cagcatcgct tcgctttgat ggtaagtttg
     1981 cctgg
//
LOCUS       DAOQJL010000658         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46822_length_1983_cov_3.063278, whole genome shotgun sequence.
ACCESSION   DAOQJL010000658 DAOQJL010000000
VERSION     DAOQJL010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46822_length_1983_cov_3.063278"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLG78_19325"
     CDS             complement(<1..374)
                     /locus_tag="PLG78_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25334.1"
                     /translation="MLWTFSKVVEMLFLKKNAPRILVGGMFLLLAAQLIWWLTFFRLK
                     QKRFEQLEDALDEQRIALMRASRGSFPDIVCNDGKCAVRPEILAERARRHAREMWMLL
                     SETMFVLSVIGYGSWLVFRSVQ"
     gene            complement(346..1632)
                     /locus_tag="PLG78_19330"
     CDS             complement(346..1632)
                     /locus_tag="PLG78_19330"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNE25335.1"
                     /translation="MNQTNSQSLYARATRVIPGGVNSPVRAFRSVGGTPIYMESATGS
                     RLRDADGNVYVDFCNSWGPLITGHRHPAILSAIRSQMLRSLTFGTPCEQEVLLAEHVI
                     QLLKRSVPDCRIEKIRFMSSGTEAVMTAIRLARGFTNRSKILKFDGCYHGHSDSLLAK
                     AGSGMATLGVPDSAGVPASFTTETIVIPLDDEAALENAFVQHGRDIAAILIEPVPANN
                     GLLLQRKEFLQKLKYMAVAGGALLIFDEVISGFRVAPGGAAQYYGICPDLVTYGKIIG
                     GGMPVGALAGRNDVMDRLAPDGPVYQAGTLSGNPVAMSAGLAQLKLLEQPAYEHLETL
                     GRNLETAFNAASLSWKMVRIGSIFWFYQGQEPPRRADKIGRDSMKVYAEFHRYCLERG
                     MYLAPSGYEVGFLSTPMTEQEVSRFLGLAVDFFKSR"
     gene            complement(1629..>1983)
                     /locus_tag="PLG78_19335"
     CDS             complement(1629..>1983)
                     /locus_tag="PLG78_19335"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNE25336.1"
                     /translation="AFRSQDGREDFMEVLGAAGRPYRDVPLYKTTHLPVDRSQLDSLP
                     ARAAVIFASPSAFLSWIKAAGGGSSASAYHLERGLRLCAIGNTTADAMLEMNCVPYAI
                     SEHPDLDAFIQELMQ"
BASE COUNT          469 a          534 c          523 g          457 t
ORIGIN      
        1 ttttgaacgg atctgaacac tagccaggat ccatacccga taacgctcag tacgaacatt
       61 gtctcagaca aaagcatcca catctcgcgc gcatggcggc gcgctctttc tgccagaatc
      121 tctggtctca ccgcgcattt tccgtcgttg catactatat cagggaaaga gcccctggat
      181 gcacgcatga gcgcaatgcg ttgctcatcg agtgcatctt ccagctgttc aaagcgcttt
      241 tgtttgagtc gaaagaaggt gagccaccag atcagctgtg ctgcgagcaa tagaaacatg
      301 ccgccgacta gaatccgcgg cgcgttcttc tttagaaaaa gcatctcaac gacttttgaa
      361 aaagtccaca gcaagtccca ggaatctaga cacttcctgt tctgtcatgg gagtgctcaa
      421 gaagccaact tcgtatccgg agggcgccag atacattccg cgttccaggc agtagcggtg
      481 gaattcagca tacactttca tggagtcgcg tccaattttg tctgcgcgcc tgggtggctc
      541 ctgtccctgg tagaaccaga agatcgaacc aatgcgcacc atcttccagg agagagaggc
      601 agcattgaac gcagtttcca gatttcgacc gagtgtttcc aggtgttcgt aagccggctg
      661 ttctagaagt ttcagctgtg cgaggcccgc agacatagcc actggattgc cggataacgt
      721 tcctgcctgg taaacgggac catccggtgc caggcgatcc atcacatcat ttcttcctgc
      781 cagggcgccc acaggcatcc cgccaccaat gatcttgccg taggtaacca ggtccgggca
      841 aattccatag tactgtgctg ctccacccgg ggccacgcga aagccgctga tgacttcgtc
      901 aaagatcaaa agtgctccac ctgcgaccgc catgtatttc agtttctgaa gaaactcttt
      961 tctctgaagc aacaaaccat tgttagctgg aacaggttca atgagaatgg cagcaatgtc
     1021 tcgaccatgc tgcacgaaag cgttttcaag agccgcttcg tcatcgagtg gaatgacaat
     1081 ggtctctgtt gtaaaagaag caggaacacc ggcggaatca ggtactccca gagtggccat
     1141 cccggatcca gctttcgcaa gcagactgtc cgagtggccg tggtagcatc cgtcgaactt
     1201 cagtatcttg ctgcgatttg taaaacccct ggccaggcga atcgcagtca tgaccgcttc
     1261 tgtcccggaa ctcatgaaac gtatcttttc aattctgcag tcaggaacgc tccttttcaa
     1321 tagctggatg acatgttctg ccaggagcac ttcctgttcg catggagttc caaatgttaa
     1381 agagcgaagc atctggctgc ggatggcgga gagaatggcc ggatgtctgt gacctgtgat
     1441 caacggtccc caggaattgc agaagtctac gtaaacgttt ccgtccgcat ctcgcagtcg
     1501 cgagccagtg gcactttcca tatagatggg cgtgccgccc acggatcgaa atgcacgtac
     1561 cggactgttg actccacccg ggatcacacg cgtggcgcga gcgtatagac tttgtgagtt
     1621 tgtctggttc attgcattag ctcctgtata aatgcgtcaa gatccggatg ctccgaaatg
     1681 gcgtaaggca cgcaattcat ttctaacatt gcatccgcag tagtatttcc aatggcgcac
     1741 agcctcaggc cgcgttcgag gtggtaggcg gaggcagaag agccacctcc cgccgctttt
     1801 atccaggaaa ggaatgcaga aggactggcg aaaatgacgg cagcgcgagc aggcagcgag
     1861 tccagttgag atcggtctac tggcaggtgg gtggttttgt agagagggac gtcccggtag
     1921 ggtctgcctg cagctcccag tacttccatg aagtcttctc ttccgtcctg ggaacgaaac
     1981 gcg
//
LOCUS       DAOQJL010000659         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_46935_length_1980_cov_4.665974, whole genome shotgun sequence.
ACCESSION   DAOQJL010000659 DAOQJL010000000
VERSION     DAOQJL010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46935_length_1980_cov_4.665974"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLG78_19340"
     CDS             complement(<1..365)
                     /locus_tag="PLG78_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25337.1"
                     /translation="MKFASLLLLFLLSCTTYYAGVKAPAEDAKLLQIAYRKPPLLIPK
                     VNGVSYGFLLNPIFFTQPGRNDLDVLYSVVTGANSSIGSRETIPVSVEGAGGTSIVVC
                     DQTAPDMSSFSVHFYSVPF"
     gene            complement(362..976)
                     /locus_tag="PLG78_19345"
     CDS             complement(362..976)
                     /locus_tag="PLG78_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25338.1"
                     /translation="MQESQRKIPRIGKWLTIVVLTSTMPSCITWYTGKPDQTTTLDNK
                     EEFHFSVTSYGEAGVGAIAKADVAVAEMLRKFGYTRAVQEKSRLDYQNKGGRDPFIEI
                     ACSQSHVVRWQSASAYWAVASLGTLGVIPHFGSQDLQVDVALYHFDATKGSFVAKRIR
                     SYNMKGYRFSGILALPVFWVSWIADGEKEVVRRAVLDFLQEPLI"
     gene            complement(943..1200)
                     /locus_tag="PLG78_19350"
     CDS             complement(943..1200)
                     /locus_tag="PLG78_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25339.1"
                     /translation="MPENESIFPASGCDHCGGQEAMKAAMFLLLAVCACKSFETVVLV
                     PTNQGTYRIARIDWAISDEAEFCKREEGRIKCKKVNVRFPE"
     gene            complement(1231..1416)
                     /locus_tag="PLG78_19355"
     CDS             complement(1231..1416)
                     /locus_tag="PLG78_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25340.1"
                     /translation="MEKVNEETTLINHTLYELYDRMHNYVRPPGSTGIVRRKAHYGQK
                     IRTHILLFLGQPSLDRR"
     gene            complement(1656..>1980)
                     /locus_tag="PLG78_19360"
     CDS             complement(1656..>1980)
                     /locus_tag="PLG78_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25341.1"
                     /translation="PKAFLRPLGLFPGAFTFYLRRIERPIQAHLAHREYILTDTLNGQ
                     IDYPFRKSWRLKCAANHEATDKSKDHSFLRGENSVSFTFLQEYFLTATTIMNQVDKHL
                     CPGLK"
BASE COUNT          477 a          488 c          513 g          502 t
ORIGIN      
        1 tgaaatggaa cagaatagaa gtgaacgcta aatgagctca tgtctggagc agtttgatcg
       61 cacacaacaa tggaagtccc accggcaccc tctacgctca cgggaatagt ttcgcgcgac
      121 cctattgagg aatttgctcc tgtgactact gagtagagta cgtccagatc gttgcgaccc
      181 ggctgcgtaa aaaagatcgg gttcaggagg aaaccgtacg agacgccgtt cacctttgga
      241 atcagtaaag gcggcttgcg gtaggcgatc tgcagaagct ttgcgtcttc tgctggagcc
      301 ttcactcccg cgtagtaagt tgtgcaagag agaagaaaaa gtagaagcaa agatgcaaat
      361 ttcaaattaa gggctcctgt agaaaatcaa gaactgcgcg ccgaacgacc tccttttcgc
      421 catccgcgat ccaggacacc cagaaaacgg ggagagcgag gatgccggag aagcggtagc
      481 ctttcatgtt gtaagagcgg attctttttg cgacgaacga tccctttgtc gcatcgaagt
      541 gatagagagc cacatcgact tgtagatcct gcgacccgaa atgggggatg actcccagcg
      601 ttcccaggga agcaactgcc cagtaggcag aggcgctctg ccaccgcact acatgggact
      661 gcgaacacgc aatctcaata aaaggatctc gtccgccctt attctgataa tccagtcgcg
      721 acttttcctg cacggcccgg gtgtagccaa atttccgcaa catctcggcc acggctacgt
      781 ctgcttttgc tattgctccg actccagctt cgccgtatga cgtaacggaa aagtgaaatt
      841 cctctttgtt atcaagggtg gttgtctggt ctggttttcc tgtgtaccac gtgatacaac
      901 tcggcatcgt cgatgttaat actacgatag tgagccactt tcctattcgg ggaatcttac
      961 gttgactttc ttgcatttta ttcttccctc ctcgcgcttg caaaactctg cttcgtccga
     1021 aatggcccag tcaatgcgag caatccgata agttccctgg ttcgttggta ccagaacaac
     1081 ggtttcgaac gatttgcacg cgcacactgc cagcagtagg aacatggctg ctttcatagc
     1141 ttcttgccct ccacaatgat cgcatcccga agctggaaag atggattcat tttccggcat
     1201 tgcaggatat tcgatgcact cacttcgcat tcaacgtcga tccaatgaag gctggcccag
     1261 aaacagtaga atgtgagttc ggattttctg gccgtaatgc gctttccgtc ggacgatccc
     1321 cgtggatcca ggaggcctga cgtagttgtg catgcgatcg tagagctcat acagagtgtg
     1381 attgataaga gtagtctctt cattgacctt ctccatccgg ccattcgacc atcatctttg
     1441 tgcattcaag gtcctggttg gcctgaatct tgcacctagc aatgtacggc tcaagtgcgt
     1501 ttccgctgcc cggacgtggc tgtgccattg cagagatcaa gattgagtct tctgcgaagg
     1561 gtactgcact gtatacgtgc gtagcgctct gacagctgct caagcaagcc acagaaatcg
     1621 gcaacaagag ttttccaaca tttgtcaaca tgggctcatt ttagtcctgg acataggtgc
     1681 ttgtcgactt gattcatgat tgtggtcgcg gttagaaaat attcctgcag aaaagtgaag
     1741 gagacggaat tctctccgcg taggaacgaa tgatctttag atttgtctgt tgcttcgtgg
     1801 tttgctgcgc acttcagtct ccagcttttg cggaaagggt agtcgatttg accgttcagg
     1861 gtatccgtga ggatatactc tcgatgagcg aggtgtgcct gaatgggcct ttcgatccgg
     1921 cgaaggtaga aagtgaatgc acctggaaag agacccaatg ggcgcaggaa agctttgggt
//
LOCUS       DAOQJL010000660         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47074_length_1977_cov_3.864204, whole genome shotgun sequence.
ACCESSION   DAOQJL010000660 DAOQJL010000000
VERSION     DAOQJL010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47074_length_1977_cov_3.864204"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1977)
                     /locus_tag="PLG78_19365"
     CDS             complement(<1..>1977)
                     /locus_tag="PLG78_19365"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE25342.1"
                     /translation="LKNLKITHRLLILIVVFTIPLGVLAFLEISSLVEVIHFKRLRRT
                     AFVMMHPMIDARTKINFRRGIMALVARGEGSKDLLPDVDRFIDDQMEEENKLITQYGD
                     IFETKKAYVEARQAWEKLKQAGTGQSLESSYRSHTELSLALLAISRTYGSASTLDLEE
                     EGMLYYPMWISTSEVPRIYDNMFQLMSRTFDAVEDDKVDSFERASLRELVVNLEESRD
                     ALKAELKTTDENYPGYLSRVSNKFQESEKRQDELANFVRRNVLEGDGKVDRKMMIAYA
                     SAAADKLEAFHDENGLALKEGYDTLERKKQYLLALLIALISTAMGVALFLGYLTTRNL
                     SRSLGYVIQNMDSIGSGKLDNQIDAQSKDEIGTILKYLASMQSNLRGTLEENLRVRTA
                     VDNSSTMVMIADTNRAIVYTNNAMEEFFRKAEKDIQKDIPSFDRNRVLGMKFDQFSTK
                     GTSLTDLERSEVPLGGHILRVSTSAIVNVIGERLGTVLEWTDRTNEVHAEQEIESLVS
                     AAVRGDFAMRMDLQGKQGFFRKLGAEINNLMQVSASGLADVARVLGAMAEGNLTERIT
                     ADYSGTFAELKDFSNNTATRLAEIIARVRESAETLASASEEVSATAQSLSQGSSEQAA
                     SVEETGSSLEEMSATISQNADNARTTNEIANRTS"
BASE COUNT          448 a          526 c          456 g          547 t
ORIGIN      
        1 ttcgacgtgc gatttgcaat ctcattggtg gttcgtgcgt tatccgcatt ctgagagatc
       61 gttgcgctca tttcttctag agaagaacca gtctcctcca cactcgcagc ctgttcgctg
      121 gatccctgac tcagactctg cgctgtggcg ctcacttctt ccgatgcact tgcaagagtc
      181 tctgccgatt cgcgaactcg agcaatgatc tccgccagcc gcgtggctgt gttgtttgag
      241 aaatctttaa gctccgcaaa cgtgccactg taatctgccg ttatgcgctc tgtgagattg
      301 ccctctgcca tggcaccgag aacccgggcc acatccgcga gaccagaggc gctcacctgc
      361 atgagattgt tgatctctgc acccagtttg cggaaaaatc cctgcttgcc ctgcaaatcc
      421 atgcgcatgg cgaaatcccc gcgcactgcc gcagatacga gcgattcgat ttcctgctct
      481 gcatgaactt cgttggttct gtccgtccat tccagaaccg ttcccagacg ctcgccgatt
      541 acgtttacga tggcacttgt ggatacgcga agaatatgtc cgcccagagg cacttctgat
      601 ctttccagat ccgtgagact ggttcctttt gtgctaaact gatcgaactt cattcctaga
      661 acccggttgc gatcaaaaga cggaatatcc ttctgaatgt ccttctccgc tttgcggaag
      721 aactcttcca ttgcgttgtt cgtatatacg atcgctcgat tggtgtccgc gatcatcacc
      781 atggtagacg agttgtctac tgcagtgcgc actctgaggt tttcttccag cgtgcccctt
      841 agatttgact gcatgctggc caggtatttt agaatcgtgc cgatctcatc tttgctctgg
      901 gcatcaatct gattgtccag tttgcccgaa ccaatggaat ccatattctg gatcacatat
      961 ccaagagagc gcgaaaggtt ccttgtggtt aagtagccca ggaacaatgc cacacccatc
     1021 gcagtggaga tcaaagcgat gagaagagcc agcaagtatt gctttttgcg ctccagagta
     1081 tcatacccct ctttgagagc caggccgttt tcatcatgaa aggcttctag cttgtcggcg
     1141 gcagcgctcg cgtatgcgat catcatcttg cgatctacct ttccgtctcc ttcgagtaca
     1201 tttcttctta caaagtttgc cagttcgtcc tggcgtttct ctgattcctg aaacttgttt
     1261 gaaacgcgcg ataagtaccc tggatagttt tcgtcagtag tctttaattc cgctttgaga
     1321 gcatcgcgtg actcttccag attcactacg agttcgcgaa gagatgccct ttcaaaggaa
     1381 tctaccttgt catcttccac agcatcaaac gtacgagaca tgagctggaa catgttgtcg
     1441 tagatgcgag gaacctccga cgtagaaatc cacatgggat agtagagcat gccctcttct
     1501 tctagatcca gagtggacgc ggacccgtaa gttctcgaaa tagcaaggag tgcgagcgaa
     1561 agctctgtat ggctgcggta ggatgattcc agagattgac ctgtgcctgc ctgtttcagt
     1621 ttttcccagg cctgacgcgc ttctacatac gccttttttg tctcgaaaat atctccatac
     1681 tgagtgatga gtttgttctc ttcttccatt tgatcgtcta tgaaacgatc cacatccgga
     1741 agcaaatcct ttgacccctc tcccctggca acgagagcca taattccgcg gcgaaaattt
     1801 attttagtcc gcgcatcaat cattggatgc atcattacga aagcggttcg gcgaagacgt
     1861 ttgaagtgaa tcacttccac cagactggaa atctcgagga acgcgagaac acccagcggt
     1921 atggtaaaga caacaattag aatcagaagc ctgtgagtga ttttaagatt ctttaac
//
LOCUS       DAOQJL010000661         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47224_length_1974_cov_4.480980, whole genome shotgun sequence.
ACCESSION   DAOQJL010000661 DAOQJL010000000
VERSION     DAOQJL010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47224_length_1974_cov_4.480980"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLG78_19370"
     CDS             <1..488
                     /locus_tag="PLG78_19370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE25343.1"
                     /translation="PYLGYEKFEFKVPIYNEGDSYARYIIRLDEMDESCKIVRQALAE
                     LKPGPINYEDQKHVLPDKNSVYHDMESLIHHFKVIMPGGKHGIQPPRKTMYAATEAPN
                     GELGFYIISDGGANPYKVRVRPPSLFNYQIYSKLMTGANLSDLVTILASFNVIAGELD
                     R"
     gene            466..1569
                     /locus_tag="PLG78_19375"
     CDS             466..1569
                     /locus_tag="PLG78_19375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE25344.1"
                     /translation="MLANSIDSAALHPLHFVFTGGVLSALFAAGYWISGQKRSRVLAA
                     IFLCLAVVLIHGYLVLSREILLVPRLLYVHVPFLYLLGPLSFLWLRLLLEEDPPHKWL
                     HYIPAALMLVLMTPLYLADPEWKRSLALGFYQGNVPLPARFVFGGGLLSYVIYIVLSV
                     RFLWPSLRSPDFYKDRSVRIAGLILIYATLICLVAFLSLLLGFPVALHWMLMMLAAFP
                     PALYLLGARYPDLFFEFKTALSKGRYEQSRLQGMDLGDLRSRIERLMREEELFGEEKL
                     TIEDVAGRLGISVHALSEFINLHEKTNFPGYINALRIGRACELMENDPARTILSIAYD
                     VGFSAKSTFNAAFLRHRGMSPTAFRAKLQKRVR"
     gene            1747..>1974
                     /locus_tag="PLG78_19380"
     CDS             1747..>1974
                     /locus_tag="PLG78_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase"
                     /protein_id="HNE25345.1"
                     /translation="MNFVRYYPIAGLAFLIFWVLGKKAFALRRVQDSFPKAERIWYEI
                     KYSFGTLVIFTGIGLFSVYLRHFGLTKVYVDL"
BASE COUNT          440 a          490 c          461 g          583 t
ORIGIN      
        1 agccatatct tggttatgaa aaattcgaat tcaaagtacc catttacaac gaaggtgaca
       61 gttacgcgcg ttatatcatt cgcctcgacg aaatggacga aagttgcaag atcgttcgtc
      121 aggcactcgc agagctaaaa ccaggaccca ttaattacga agatcagaag catgtcctgc
      181 cggacaagaa cagtgtctat cacgacatgg aatcgctcat ccatcatttc aaagtgatca
      241 tgccgggagg caaacatgga attcaacctc ccaggaaaac aatgtatgcg gccacagaag
      301 cgcctaacgg tgaactggga ttctacatca tttcagatgg cggtgcaaat ccatacaaag
      361 ttcgagtccg tcctccttct ctttttaact accagatcta cagcaaactc atgacaggcg
      421 cgaatctctc tgacctggtc acgatccttg cttctttcaa cgtaattgct ggcgaactcg
      481 atcgatagcg cggcacttca tcctctgcac tttgtcttca caggcggagt gctgagcgct
      541 ctttttgctg caggttactg gatttccgga caaaaaagaa gtcgcgtgct cgctgcgatt
      601 tttctttgcc tcgctgtggt tttgatccat ggatacctgg ttctcagtcg cgagattctt
      661 ctggttccga ggctcctcta cgttcatgtt ccgtttttat atttgcttgg accgttatcg
      721 ttcttgtggt tgcgccttct tctggaagaa gatccgccgc acaaatggct ccactatatc
      781 ccggccgcgc tcatgcttgt tttaatgact ccattgtatc tggcggatcc cgaatggaaa
      841 cgctctcttg ccctgggatt ctatcaaggc aatgtccctc ttcccgccag gtttgtattc
      901 ggaggcggac ttctttccta tgtaatctat attgttcttt ctgttcggtt tctatggcct
      961 tctctccggt cccctgactt ttacaaagat cgaagcgttc gcattgcagg cctcattctg
     1021 atttacgcta cactcatctg tctggttgcg ttcctttctc ttctactggg ctttccggtt
     1081 gctcttcact ggatgctgat gatgctcgct gcatttcctc cggctctcta tcttctgggg
     1141 gcccgttatc ccgacttgtt ttttgaattc aagacagcct tgagcaaagg cagatacgag
     1201 cagtcgagac tccagggaat ggatctcgga gatttgcgat ctcgcattga aaggctaatg
     1261 cgcgaagagg aattgtttgg agaggaaaaa ctgactattg aagacgtggc aggcaggctc
     1321 ggaatcagtg tgcatgcgct cagcgagttc atcaatttgc acgagaagac gaatttcccc
     1381 ggttacatca atgcccttcg cattgggcgc gcgtgcgagc ttatggaaaa tgatcccgca
     1441 cgaacgattc tttctattgc ttatgatgta ggtttttccg cgaagtccac attcaatgcc
     1501 gcttttttgc gtcatcgcgg gatgagccca actgcgtttc gcgctaaatt gcagaagcgc
     1561 gtgcgctaaa aacagctccc gaaggcaaaa cgggtccagt gttataaatc tggacgcctt
     1621 ttttgttcca ggtttatact accggtcgat tcgcaaaggc ctctgtggta tgcttcgatc
     1681 atgaactttc tacaatgcgg aatcaccctc gactgttttg aaaaggcggg atcattctta
     1741 ttgaccatga actttgtgag gtattatcca attgctggcc tcgcattttt gattttctgg
     1801 gtgctcggca agaaggcgtt tgccctgcga agagtacagg attctttccc gaaggcggaa
     1861 cgaatctggt atgagatcaa gtactctttc ggtactctcg taatctttac tggcattggc
     1921 ctgttctcag tctatctcag gcattttggt ctgactaaag tctacgtcga tctg
//
LOCUS       DAOQJL010000662         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47282_length_1973_cov_3.568300, whole genome shotgun sequence.
ACCESSION   DAOQJL010000662 DAOQJL010000000
VERSION     DAOQJL010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47282_length_1973_cov_3.568300"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLG78_19385"
     CDS             complement(<1..572)
                     /locus_tag="PLG78_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE25346.1"
                     /translation="MSNKEFDFVVYGASGFTGRLVAEYLVQRGIRGWAMAGRSQGKLA
                     EVRDLVGAPKDTSLIEADASNPSSLSAMCDRAKIILTTVGPYQLYGSELVAACAASGT
                     DYLDLCGEPAWMRHMIDAHEAAAIKSGARIVFSAGFDSIPFDLGVWYLQQAAIAKFGS
                     PAPRVKGRVRKMAGGASGGTMASMKATLRAS"
     gene            666..1199
                     /locus_tag="PLG78_19390"
     CDS             666..1199
                     /locus_tag="PLG78_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25347.1"
                     /translation="MHPPQLTMSRAAFLRGSIKLFALTSLTMSGVDCGPSSIPSLKGI
                     SAQEYRNMNAVGEVFLADSPFPDFDLGQALDDYVFGHPSPLDTKDIVRELAGAPSSYL
                     AAIVLDGSMTPLVKLSRADREARMLGWKKSDNIMKRGLYNILRQTTFFLLSSSPALPR
                     YAGYAIQGDLVPYGGGM"
     gene            1196..>1973
                     /locus_tag="PLG78_19395"
     CDS             1196..>1973
                     /locus_tag="PLG78_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE25348.1"
                     /translation="MKKQIITPAEHKDIIKEFDLGRKWSLKADAVVIGSGAGGAVTAA
                     ELAKNGWKVVLIEEGSYFHPGIFSNDEFLSQARLYRDAGYVLTENQTASIVQGRTLGG
                     STTVNWQTSLYPPSYVTDEWRDRFGLPGYGRKEEISSYVEEVHKRLNVHEVPTKLINR
                     NNGVLMRGGRKLGLHPEVLKNNNNGCKGLGRCGLGCPINAKQSMFLTYIPDAIAAGAI
                     VVTNMRAVKISDGKTKRVTAEFTPDPYQASPEEIIEEMQIE"
BASE COUNT          495 a          515 c          504 g          459 t
ORIGIN      
        1 gatgcacgca gagtcgcctt catgctggcc atggttccgc cggaagctcc gcccgccatc
       61 ttgcgcactc ttcctttgac tctgggtgca ggggatccaa acttcgcaat ggcggcttgc
      121 tgcaaatacc atacgccaag atcaaatgga atggaatcaa atcccgctga aaaaacaatg
      181 cgcgctccgc tcttgattgc ggctgcttca tgagcgtcga tcatatggcg catccacgcc
      241 ggttccccgc ataaatccag ataatcggtt ccacttgcag cacaggcggc cacaagttct
      301 gagccataca gctggtaagg acctacagtt gtaagaatga tcttcgcgcg atcgcacatg
      361 gcgctcaaag aggatggatt ggatgcatct gcttctatga gagatgtatc ttttggggcg
      421 cccacaaggt ctctgacttc agccagtttg ccctggcttc ttcccgccat ggcccagccg
      481 cgaatgcctc tttgcaccag atactcggcc acgagcctgc cggtgaaacc gcttgctccg
      541 tacactacaa aatcaaattc cttgttgctc ataaccacct ttaggctcgg tcgaataaac
      601 tgtcaaagtc gattctaacc tatgttcgag tttgacgatt ttcgggcccg ccagcagaaa
      661 cgaccatgca tcctccccag ctcacgatgt cccgggccgc tttcttgcgc ggttcgatca
      721 agctgtttgc tcttacatct ctgactatgt caggagtaga ctgcggcccc tccagcatcc
      781 cttctctgaa aggaatcagc gcacaggaat atcgcaacat gaacgcggta ggtgaagtgt
      841 tccttgcgga ttcacctttt cctgattttg atctgggtca ggcgctcgac gactatgtgt
      901 tcggtcatcc ttcgcctctg gatacaaagg acattgtgcg cgaactggca ggagctcctt
      961 cttcttatct ggccgccatc gtgctggatg gatctatgac tccgctggtt aaactatctc
     1021 gcgcggacag ggaagcaaga atgctcggct ggaagaagtc ggacaacatc atgaagagag
     1081 gtctttataa tatactgagg caaacaacgt tctttctact ttcatccagt ccggcgcttc
     1141 cgcgttacgc cggttatgcg atccagggag atctggtacc ttacggaggt ggaatgtgaa
     1201 aaagcaaatc atcactccag cagaacacaa agacatcatc aaagaatttg atctcgggcg
     1261 aaagtggagt ctgaaagcag acgcagtcgt cataggttct ggagcgggcg gtgctgtgac
     1321 cgcagcagaa ctggcgaaga acggttggaa ggtggtcctg atcgaagaag ggagttactt
     1381 tcatccggga attttttcaa acgacgaatt tttgtctcag gcaaggctct atcgcgatgc
     1441 aggctacgtg ctgactgaaa accagacggc aagcattgtg cagggcagaa ctctgggtgg
     1501 atctaccaca gtcaactggc agacttctct gtatcctcca tcgtatgtaa cagacgaatg
     1561 gcgggacagg tttggacttc caggttacgg aagaaaagaa gagatttctt cgtacgtaga
     1621 agaggtccac aaaagactca acgttcacga agtccccacg aaattgatca acagaaacaa
     1681 tggagtgctc atgcgcggtg gtcgcaagct tggcctccat cctgaagtgc tcaagaacaa
     1741 caacaacggt tgcaaaggtc ttggacgatg cggtctgggc tgtccgatca acgcaaagca
     1801 gtccatgttt ttgacctaca ttccggatgc gattgccgcg ggcgccattg tagtgaccaa
     1861 catgcgcgct gttaaaatca gcgatggcaa aacaaaacga gtcacggcag aattcactcc
     1921 agatccatac caggcttcgc ctgaagagat catcgaagag atgcaaattg aag
//
LOCUS       DAOQJL010000663         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47332_length_1972_cov_2.864893, whole genome shotgun sequence.
ACCESSION   DAOQJL010000663 DAOQJL010000000
VERSION     DAOQJL010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47332_length_1972_cov_2.864893"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLG78_19400"
     CDS             complement(<1..986)
                     /locus_tag="PLG78_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE25349.1"
                     /translation="MSVDQKLIRNFSIIAHIDHGKSTLADRILGLGGVTNARTSKDQI
                     LDSMDIERERGITIKMNSATFNYKTTDGITYIYNLIDTPGHVDFTYEVSRSLAACEGV
                     LLVVDASQGVEAQTLANLYLAMEANLRIIPVINKIDLPAADVPRCLDLIEKTLGLDPT
                     EAIAVSAKTGLNVPAILESIAKLIPAPSGDLNAPLRAMIYDSYYDSYCGAVMKVRIVD
                     GILRKGDRVSFMSHPRDYQVTEVGITAITNFPKDELAAGEVGYVIAGIKSVEDTRVGD
                     TVTLTSRPASVALPGYRDAKPMVFAGLFPVNGEDYPQLKDAIVKLKMNDAALT"
     gene            1315..>1972
                     /locus_tag="PLG78_19405"
     CDS             1315..>1972
                     /locus_tag="PLG78_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25350.1"
                     /translation="MRTGWLLCVCLVCAASARSLADTVVLRSGVQLRNVKTSPIGEMH
                     LIEFEDGTIEKIPNSRIRSLRRGATTWTRNQVKEPEAPKVVTPEPSLPASRRSLAPIL
                     KSAVLPGWGQISEDRVKTGMAYGAASILVFQRYWTYRQRHAAAQRDYNDPVPVGLVAS
                     QSVSGSLTLPQAAAINLAYLSQKEKQVYSLQRKGNNMLALLPAIWAWNLLDIVYGGVP
                     W"
BASE COUNT          423 a          493 c          571 g          485 t
ORIGIN      
        1 gtgagtgccg cgtcattcat tttcagtttg acgatggcat ctttcagttg cggataatct
       61 tctccgttca caggaaacag tccggcaaaa accattggtt ttgcgtcgcg gtaccctggc
      121 agtgccacag acgcagggcg agaggtgagg gtgactgtat ctcccactcg agtgtcttcc
      181 acagatttaa ttcccgcaat tacataacct acttcgccag ccgccagttc atctttcggg
      241 aaattggtga tggctgtgat tccaacctct gtgacctggt agtcgcgagg atgactcatg
      301 aagctgacgc gatcgccttt gcgcagtatg ccgtctacga ttctgacttt catcaccgcg
      361 ccgcaatagc tgtcatagta ggagtcgtag atcatggcgc gaaggggtgc attcagatcg
      421 cctgacggtg cgggaatgag cttcgcgatg gattctagaa ttgctggaac gttcagacca
      481 gtcttcgcag agactgcgat ggcttccgtt ggatcgaggc cgagtgtctt ttcaatcaga
      541 tccaggcacc gcggcacatc tgccgctggc aaatcgatct tgttgatgac cggaatgatg
      601 cgaaggttcg cttccatggc cagatagaga ttggcaagtg tttgggcctc cactccctgg
      661 cttgcgtcta ccaccaggag aaccccttcg caggcagcca gagaacgaga tacttcgtat
      721 gtgaagtcca cgtgtccagg tgtgtcgatc agattgtaga tgtaagtgat tccgtccgtg
      781 gtcttgtagt tgaaggttgc ggaattcatt ttgattgtga tgccgcgctc tctctctatg
      841 tccatggaat cgagaatttg atccttgctc gtgcgtgcgt ttgtaacgcc cccgagaccc
      901 aggatcctat ctgccagagt ggacttgcca tggtcgatgt gggcaatgat ggaaaagttg
      961 cgaatgagtt tctggtcgac gctcatagtg ggttacggca ttttttctga agcgccgggg
     1021 tgaagaaccc ttttttcggg cacacgcgcc ggtctccgat gggccaggat ttcctggatt
     1081 cggggcgcaa gccgggcaag tcgaggccca ggccatattt caggacgatt cgaggcggca
     1141 ccgtgggcgc agaggaacag cgacgggcgg ggcccggaat gaatgtatcg gaagatgtca
     1201 tgactaccag tgaactatag ttagatttat ttactattcg gagaggatag atcgctttcc
     1261 tggatgggtg cgaaaagcac tttgcctcac cgcgcggaag gccaacgttg ccatgtgagg
     1321 acgggatggc ttctatgcgt ctgtcttgtg tgcgctgcca gtgcgcgatc actcgcggat
     1381 acggttgtac tccgttcagg cgtccagctc cgcaatgtga agacttcgcc aatcggcgaa
     1441 atgcacctca ttgagttcga agacggcacc atagagaaaa ttccaaattc gcgcatccgt
     1501 tctcttcgca gaggggccac gacatggact agaaatcagg tcaaagagcc ggaagctccg
     1561 aaggttgtta ctccagagcc ttctcttccc gcgtcgcgcc gctctctggc cccgattctc
     1621 aaatcggcgg tgcttcctgg ctggggtcag atttctgaag atcgcgtgaa aacagggatg
     1681 gcttatggag cagcgtctat cctggtgttt cagaggtact ggacttatag acagaggcat
     1741 gcggcggccc agagagatta taacgacccg gtccccgtgg gtttagtcgc ctcgcagagc
     1801 gtctcaggat ctttgactct cccgcaagcg gcggcgatca atctagcata tctatcccag
     1861 aaagagaagc aagtgtacag tctgcagaga aaagggaaca acatgcttgc gctcttgcct
     1921 gcgatatggg cctggaactt gcttgacatt gtttacgggg gagttccctg gg
//
LOCUS       DAOQJL010000664         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47361_length_1971_cov_4.651879, whole genome shotgun sequence.
ACCESSION   DAOQJL010000664 DAOQJL010000000
VERSION     DAOQJL010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47361_length_1971_cov_4.651879"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="argF"
                     /locus_tag="PLG78_19410"
     CDS             <1..776
                     /gene="argF"
                     /locus_tag="PLG78_19410"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE25351.1"
                     /translation="KSSTRTRLSFSVAVTEMGGTPISLEAMQLQLGRGESLEDTTEVL
                     ARYCHAMMVRARDHRNLEVMAGMNRIPIINGLSDLHHPCQIIADYMTLEQLGFSLHGG
                     LRIAFVGEPNNVFNSLAVAASYAKARITIASPRAYSQIHPAIQQELTRLKTEFQQFTE
                     PAGAVRDTQVVYTDTWVSMGQEAEEETRRKDFADYCVTTELLSLAASGHVVLHCLPAH
                     RGEEITAEVMEKYKHSIFEQAENRLHAQKAILEWIFGIL"
     gene            complement(773..1810)
                     /locus_tag="PLG78_19415"
     CDS             complement(773..1810)
                     /locus_tag="PLG78_19415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNE25352.1"
                     /translation="MGDVALLAPVLAALAKEHSRIAITLVTRKAFAPFFYNIPGVEVI
                     GADVDKEYKGLLGLYKLYRELMALGPYDHGIDVHGSIRSRILRLYFRLSGLKFSAIVK
                     GRREKSLQVRRYNKVLEPLPHMVERYMHVFDRAGISADPGRGPWINPDTRCRALAMDY
                     LKKINKPNKTNFWIGIAPFAGHAQKMWPLDKMRELVGLLSKLDATVFLFGGKGKEKEI
                     LESIHAEHPEKTHVVAGNLTLEGEMALILRLDVMIAMDSFNMHIASLLGTSTISIWGS
                     THPFSGFGPYGPNSVIVQIDPSVLPCRPCSIFGNKPCYRKDLACMNWISAERVFEETT
                     KLLDIGPAQTV"
BASE COUNT          502 a          512 c          474 g          483 t
ORIGIN      
        1 aaaagtcatc cacgcgcacc cgtttgagtt tcagtgttgc agtcactgag atgggtggaa
       61 cgcccatttc tctggaagcc atgcagctac aactcggccg cggtgaaagc ctggaggaca
      121 caacggaagt cctcgcacgc tactgtcacg ctatgatggt tcgtgctcga gatcatagaa
      181 acctggaagt gatggccggg atgaatcgca ttccgatcat caacggtctg tccgatcttc
      241 accacccctg ccagattata gctgactata tgaccctcga gcaattgggc ttcagcctgc
      301 acggtggtct tcgcattgcg tttgtcgggg agccaaataa cgttttcaat tctctggcag
      361 tggcggcttc gtatgcgaaa gcgcgcatta cgatcgcctc acctcgcgct tacagtcaga
      421 ttcaccccgc aatacagcag gaactcacaa ggctcaagac tgagtttcaa cagttcacgg
      481 aaccggccgg tgcagtacgc gatacgcagg tggtctatac tgatacctgg gtttccatgg
      541 gccaggaagc agaagaagag acgcgaagaa aggactttgc agactattgc gtcaccactg
      601 aacttctatc tctcgcagcc agtggacatg tagtgcttca ttgtctgcca gctcatcgcg
      661 gagaagaaat cacagcagaa gtcatggaaa aatacaaaca ttccatcttc gaacaggctg
      721 agaacaggtt acacgcacaa aaagcgattc tggaatggat ctttggaatt ctctagactg
      781 tttgtgcggg tccgatgtcc agtagctttg tagtctcttc gaacactctc tctgcggaaa
      841 tccagttcat gcacgccaga tctttgcgat agcatggctt atttccaaag atagaacacg
      901 gcctgcacgg caacacggat ggatcaatct gaacgatcac cgaattaggt ccgtagggac
      961 caaatccaga aaaaggatga gtggatcccc agatcgaaat ggtgctggtt ccgagaagag
     1021 aagcgatgtg catgttgaaa gagtccatcg caatcatcac atccagacgc agaatgagcg
     1081 ccatttcccc ttccagtgtg agatttccgg ccactacatg agtcttctca ggatgctctg
     1141 cgtgaatgga ttctagtatt tccttttctt ttccttttcc gccaaacaaa aacactgtgg
     1201 catcgagctt cgaaaggaga cctacaagct cccgcatctt gtccagaggc cacattttct
     1261 gcgcatgtcc cgcgaatggc gcaatgccga tccagaaatt ggttttgttg ggtttattta
     1321 tcttcttgag atagtccatc gcaagtgcac gacagcgtgt atcaggattg atccacggcc
     1381 ctcttcccgg atctgcggaa atgcctgctc tatcaaagac gtgcatgtag cgctcaacca
     1441 tgtgaggaag cggctcaagc actttattgt agcgacgcac ctgcagagac ttctctcttc
     1501 ttcctttcac gattgcggag aatttcaagc cggacagacg gaagtacaaa cgcagaattc
     1561 tgcttcgaat gctgccatgc acatcaattc catgatcata cggacccagg gccatcagtt
     1621 ctctatacag cttgtaaaga ccaagcagtc ctttgtattc tttgtccaca tctgcgccga
     1681 tcacttctac gcctggaata ttgtagaaaa aaggagcaaa tgccttgcgc gtaacgagtg
     1741 tgatggcgat gcgcgagtgc tcttttgcca gggccgcaag aaccggagca agtagcgcca
     1801 catcgcccat ggcggagaag cgaagaacga ggtatctcaa atcaggcctt cgctccgccg
     1861 tacagaatcg gattgagctt tggatcgttg tacatcttca tctgccgata tactttcatc
     1921 tttttaatac ctttttcaag gtcatctacc agctcatcaa agcattgcgc c
//
LOCUS       DAOQJL010000665         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47625_length_1966_cov_3.895866, whole genome shotgun sequence.
ACCESSION   DAOQJL010000665 DAOQJL010000000
VERSION     DAOQJL010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47625_length_1966_cov_3.895866"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1261)
                     /locus_tag="PLG78_19420"
     CDS             complement(71..1261)
                     /locus_tag="PLG78_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE25353.1"
                     /translation="MKKKVLSISLGWEQEPHLKRLMSMPDLELYGIHYTEDAVHASSF
                     HERIHGDLRDLSTMLEFADRIQPSAVISDEDDYGILLAAFAAEKLGLPGPRVSEAQLG
                     VNKYLQRCRALESGLVIPRFSLCKSADDVRRFISAHGSPVILKPIDSRGSIGASKITD
                     ESQASAAFYKAVSASPSLLAIVEEYIDGDHFNVDGYCFSGKGPSSLAVSANYKLAAAD
                     GLVNDSIVYGDVKSELRHVLLRAGEETARSLGYAFGFFHGEFIRKKSDGKIFLTEMAN
                     RGGGILISQVLLPFVTGLDLVGIYIGDCLGEKRDSHVSAAENFARIDFVTLEAGRIYG
                     GLAEAPNESGILAVVPFVKPGQRLPHIGNGAQRHMMMISTERDRTLVEEMKLKLKERV
                     LYNG"
     gene            complement(1258..1449)
                     /locus_tag="PLG78_19425"
     CDS             complement(1258..1449)
                     /locus_tag="PLG78_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25354.1"
                     /translation="MKERTIKVGSREIGGKRTFLIGDIGSNHNQDIHLPYETIDAGVA
                     RADLKAGSVLNWNELEGKL"
     gene            complement(1446..>1966)
                     /locus_tag="PLG78_19430"
     CDS             complement(1446..>1966)
                     /locus_tag="PLG78_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE25355.1"
                     /translation="QTAKGANEILGVRSCELLDFADNRMDGADLLDVVKAVEKRIDAL
                     RPDVIYTHFPGDLNVDHRIVSQAVATAARPVPGQTIKEIYFFEVQSSTEWQINPSANG
                     FLANHFVSLFPTPDSRYMSKKLEALALYSSEMRDFPHARSVRAVEALAALRGATIGTE
                     AAEAFMTGRVIR"
BASE COUNT          487 a          527 c          466 g          486 t
ORIGIN      
        1 gtctatgaca gcaaacccgt tctcctgata agataaggcg aaatcatctg cgacgcgaaa
       61 gcttttcaat ttacccattg tacaataccc gttcctttaa ttttagcttc atttcttcca
      121 caagcgtcct gtctctttcc gtcgaaatca tcatcatatg acgctgcgcg ccattcccga
      181 tatgcggtaa gcgctggcca ggctttacaa acggcacgac tgccaggatt cctgattcgt
      241 ttggagcttc cgcgaggcct ccgtaaattc tgccggcttc gagagtcaca aaatcgatgc
      301 gcgcaaaatt ctcggctgcg gacacgtgcg aatccctttt ctcaccaagg cagtctccaa
      361 tgtagatacc caccagatcg agccctgtga cgaatggaag caggacctgc gaaatcagga
      421 tgccgccccc tcgatttgcc atctctgtca gaaagatttt tccgtctgac tttttgcgaa
      481 tgaattcacc atgaaaaaaa ccaaatgcat agccaagact tcttgcagtt tcttcaccgg
      541 cgcggaggag aacgtggcgg agctcagatt tcacgtcgcc ataaacgatc gaatcattta
      601 ccaggccgtc tgccgcggcg agtttgtaat tcgcagaaac agcaagagaa gaaggaccct
      661 tgccggaaaa acagtaacca tctacgttga aatgatcgcc gtctatgtac tcttctacaa
      721 ttgcaagcag agacggactg gcggaaaccg ctttgtaaaa tgcggccgac gcttgagact
      781 catccgtgat ctttgaagcg ccgatagatc cgcgactgtc gatcggtttg aggatgacag
      841 gagaaccatg cgccgagata aatcgcctaa cgtcatcagc agacttgcag agggaaaaac
      901 gaggaatgac gagcccgctc tctagagcgc ggcatctctg cagatactta ttgactccca
      961 gctgggcttc acttacgcga ggacctggca gacctaactt ctcggctgcg aaagcagcca
     1021 gcaaaatccc atagtcgtct tcgtccgaga tgactgcaga aggttgaatt ctatctgcaa
     1081 actcgagcat cgtgctcaga tcgcgcaggt cgccatggat gcgctcgtga aatgatgaag
     1141 cgtgtacggc atcttccgtg taatgaatac catacagttc taaatcaggc atggacatga
     1201 gccgcttcaa atgcggttcc tgttcccagc caagtgaaat actcagaacc ttcttcttca
     1261 tagcttgccc tcaagttcgt tccagttcag aacagatcct gctttaagat ctgcgcgagc
     1321 gacgcccgca tcgatggtct catatggaag gtgaatgtcc tggttgtgat tggaaccgat
     1381 atctccaatc aggaacgtgc gcttgccgcc aatttcgcgg ctgccaactt tgattgttct
     1441 ttctttcatc taattacccg gcccgtcatg aatgcttctg ctgcttctgt tccgatagtt
     1501 gcaccgcgca gagctgccag cgcttcgacc gcacgcacgg aacgagcgtg agggaaatcc
     1561 cgcatctcag acgaatacag agcgagagcc tcaagctttt ttgacatgta tctgctgtct
     1621 ggagttggaa aaagagaaac gaaatgattc gcaaggaagc cgtttgcaga aggattgatc
     1681 tgccattcag tacttgattg tacttcaaaa aagtaaattt cctttattgt ctgaccgggc
     1741 acgggtctcg cggctgttgc aacagcctgg ctgacaattc tgtgatccac attcaagtcg
     1801 cccggaaagt gcgtatagat cacgtcgggg cgcagcgcat cgattctctt ttcgactgct
     1861 ttgacaacgt ccagcaaatc cgctccgtcc attctgttgt ctgcaaaatc gagcagttcg
     1921 caactcctga cgcccagaat ctcgttcgcc ccttttgcag tttgcg
//
LOCUS       DAOQJL010000666         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47916_length_1960_cov_5.443045, whole genome shotgun sequence.
ACCESSION   DAOQJL010000666 DAOQJL010000000
VERSION     DAOQJL010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47916_length_1960_cov_5.443045"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..759)
                     /locus_tag="PLG78_19435"
     CDS             complement(46..759)
                     /locus_tag="PLG78_19435"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25356.1"
                     /translation="MRVVLILFLAAAGCKDGLARLKEPPRPEAVPAGAERDRRTGIWG
                     YKTDKDWTLYNADGKKYAEGGLVLGLRQGEWKYYNVDGHTVLSRGKFLNDWREGVWDY
                     NDTRGVLYLRMVYTQEPKRELIALLTHDYGNENGPYERHYPDGSIEETGSFQAGYYEG
                     PITRFHPNGRLLLKGQYQKDLMVGRWLYYYPDGTILREENYVQGALDGLFRSYHPDGR
                     LYVETHYEKGAVVGPVVIR"
     gene            complement(759..1499)
                     /locus_tag="PLG78_19440"
     CDS             complement(759..1499)
                     /locus_tag="PLG78_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25357.1"
                     /translation="MGSKKDKLGQAARRRLDETMGPPQGGNRSNMEDFVRDMRGEPAA
                     ASSEPAQSPESKRVSPRSDSSETGRKERPRMELKKSRRDSIYNISSDIRLEDYTDGGS
                     TSGGGIGGKARIIAGALLFVLLGLGIYYLAGWIFGPRYHLAVASQDITAENIEELEGR
                     EPSLSAGTDVHIRFQWPEEKLKATYIKVKVDRVDGGNKEEQAALGQKPPRTANYVYFK
                     GPLDPGVYHVEVMDESGSVMKDKEFKVR"
     gene            complement(1480..>1960)
                     /locus_tag="PLG78_19445"
     CDS             complement(1480..>1960)
                     /locus_tag="PLG78_19445"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE25358.1"
                     /translation="SLLSGALDGFFRLSESLRMQDLPYEFVIIDCPPNLGMVTVNAFV
                     AATHVLVPLQTAKFSLDGLAALTSTIKTVQSRFNQGLKILGGVLTMYNPRTAIAHAMI
                     EPIQEYIRVFDSKISRLVAVEEAFLMKQTVFEYEPKSRVAKEYLALKKEVLRGIEKG"
BASE COUNT          436 a          542 c          473 g          509 t
ORIGIN      
        1 ttccacactc gcccaccatg cggaaggcga atgcgaatca cgcggctacc gaatgacgac
       61 tgggccgacg actgcacctt tctcgtaatg ggtttccacg tagagcctgc cgtccggatg
      121 ataggatcgg aagagcccgt cgagcgcgcc ctgaacatag ttttcctctc gaaggatagt
      181 gccatccggg tagtaataca gccatcggcc aaccatgagg tctttttgat actggccctt
      241 caaaagtaga cgaccgtttg gatgaaaacg agtgatgggg ccttcgtagt atcccgcctg
      301 aaaagagcct gtctcttcta tgcttccgtc cggataatgc ctttcatacg gaccgttttc
      361 gtttccataa tcatgagtga gcagagcgat gagttctctc tttggctcct gagtatatac
      421 catgcgaaga tacagaacgc cgcgagtgtc gttgtaatcc cagacacctt ctctccagtc
      481 gttcaggaac ttgccgcgag acagcactgt gtgcccgtct acattgtaat acttccattc
      541 tccctggcga agacccagga caaggccgcc ttctgcgtat ttctttccgt ccgcgttgta
      601 gagagtccag tctttgtctg ttttgtatcc ccagatgccc gttcttcgat cacgctctgc
      661 accagcgggg accgcttccg gcctgggagg ttctttcagg cgagccaacc cgtccttgca
      721 tccggccgca gccagaaaca gaatcagaac gacgcgcatc agcgcacctt gaattcttta
      781 tctttcatga cgctgccgct ttcatccatc acttccacat gatagactcc aggatcgagc
      841 ggacctttga agtagacata gttcgctgta cgcggaggtt tttgaccaag cgcagcctgt
      901 tcttctttgt tgcctccgtc tacgcgatct actttgactt tgatataggt tgctttgagt
      961 ttttcctcag gccactgaaa gcgaatgtga acgtctgtgc ccgcagaaag actgggttca
     1021 cgaccttcga gttcttctat gttttccgcg gtgatgtctt gagatgcaac tgcaagatga
     1081 tagcgaggcc caaagatcca gcccgcgaga taataaattc caagtccaag caatacaaag
     1141 agaagcgcgc ctgcgatgat ccttgccttt ccaccaatgc cgccgccgga tgtgctgccg
     1201 ccgtcggtgt agtcttcgag tctgatatcg ctcgatatat tatagataga atccctgcga
     1261 gacttcttga gctccatgcg cggtctctcc ttacgtccag tttcggacga gtcgcttctc
     1321 ggggaaaccc ttttcgactc cggcgattgt gccggttcac tggaagcggc tgcgggttcc
     1381 cctctcatat ctcttacaaa atcttccata ttggaacgat tgccaccctg gggagggccc
     1441 atcgtttcat cgagcctgcg ccgcgctgcc tgacctagct tatccttttt cgatcccacg
     1501 aaggacctcc ttcttcaaag ccaggtactc tttggccact ctgcttttag gttcgtattc
     1561 aaatacagtc tgtttcatca agaacgcttc ttctacagcc acgagccgcg aaatcttaga
     1621 atcaaacact cgaatgtatt cctgaattgg ttcgatcatc gcatgggcga tggccgtcct
     1681 tggattgtac attgtcaaaa ctccgcccag tattttcaat ccctgattga aacgactctg
     1741 cactgttttg attgtgctgg tgagtgcggc cagtccgtcc agggaaaact tcgcagtctg
     1801 gaggggaacg agtacatgag tggcagccac aaacgcattt acagtgacca tgccgagatt
     1861 tggcggacaa tctatgatga cgaattcgta cggaagatcc tgcatacgaa gcgattcaga
     1921 aaggcgaaag aaaccatcca gagcgcctga aagtagactc
//
LOCUS       DAOQJL010000667         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_47917_length_1960_cov_5.238320, whole genome shotgun sequence.
ACCESSION   DAOQJL010000667 DAOQJL010000000
VERSION     DAOQJL010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47917_length_1960_cov_5.238320"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLG78_19450"
     CDS             <1..822
                     /locus_tag="PLG78_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25359.1"
                     /translation="VKQGLRTFEISIHEIAHIYFHLMPPGDFNELIVSRKNAAGKVKN
                     FRFYSNANDPGLLSLTEELVKLKPGADLRGMDRKEAFAHMKAVDTARYALFAVPIIVS
                     LVVACMLSPMLIHGLDKGSSNLSAEDLVAGKMPESRNVNLKGIALSDGMKETTTRKGT
                     KTVRMFFPVVTTDWKEGDPVHILVETGDLTDDQLNAVLASPEFKGVLRNVFWEGPSSK
                     QLKFFQENYNTRFAEKVYLVELGQPSDLFMFLIVMGISVVMLVGVTIYMKKKMAA"
     gene            complement(874..1143)
                     /locus_tag="PLG78_19455"
     CDS             complement(874..1143)
                     /locus_tag="PLG78_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNE25360.1"
                     /translation="MKLFVGNLPFSITEDALHDEFAAQGSVISTKIITDRDTGRSRGF
                     GFVEMASLKDGQAAISGLNGREMDGRQLAVSQAKPQENRGGRDRY"
     gene            complement(1309..>1960)
                     /locus_tag="PLG78_19460"
     CDS             complement(1309..>1960)
                     /locus_tag="PLG78_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE25361.1"
                     /translation="YEDFTDTVAQELGPDVQTAVLDVTLDGSVAELAEHLRRKGTKLD
                     GIVHAVAMDKTIRGGFAKNLLDVTREEFAGAMDVSAYSLIALTRSLIECEVLAPGASV
                     LSLSYLGASRVMTHPYKNIGVAKSALERITLEMAQELGLRGIRVNAIRFSPYAVSKAG
                     AAIPGLAEAVASCDSLSPLGNARPEDLARAAAFLLSPDLRITGQILHVDGGYSIGG"
BASE COUNT          495 a          481 c          506 g          478 t
ORIGIN      
        1 gtaaaacaag ggcttcgtac ttttgaaatt tcgatccacg aaatcgcgca catttacttt
       61 catttgatgc ctcctgggga ttttaatgaa ctcatcgttt ccaggaaaaa tgctgccggc
      121 aaagtgaaaa actttcgctt ctattccaac gcgaatgatc caggacttct ctctcttaca
      181 gaggagctcg tgaagctcaa accaggtgcc gacttacgcg gaatggacag gaaggaagcg
      241 tttgctcaca tgaaggcggt ggatacagcc cgttatgcgc tcttcgcggt tccgatcatc
      301 gtgagtttag tcgtggcgtg catgctcagc ccgatgctga ttcatggact ggataaagga
      361 tcttccaatc tatcagcaga agacctcgtg gcgggaaaga tgcccgaatc tagaaatgta
      421 aacctcaagg gaattgcact ttcggatgga atgaaggaaa caacgactcg caagggaaca
      481 aagacggtgc gcatgttctt tccggttgtg actacggatt ggaaagaagg agatcctgtg
      541 catatcctgg tggaaacagg ggacctcacg gatgatcaac tcaacgcggt tcttgcttcg
      601 ccagagttca aaggagtctt gcgaaatgtt ttctgggaag gaccttcttc caaacaactc
      661 aagttctttc aggaaaatta caatacacga ttcgcagaaa aggtgtacct tgtggagctg
      721 ggtcaacctt cggatctgtt catgttcttg attgtgatgg ggatcagcgt tgtgatgctg
      781 gttggagtaa ccatctacat gaaaaagaaa atggccgcct gaataggcgg cctttcctca
      841 aagtgaattc ggaagaaccg aattcaaaaa gaatcaataa cggtcgcggc cgcctctgtt
      901 ttcctgtggt tttgcctggc ttactgcgag ctgacgtccg tccatctcgc ggccgttgag
      961 gccggagatt gctgcctgac cgtctttgag gcttgccatt tcaacaaatc cgaaaccgcg
     1021 tgaacggcct gtgtcgcgat cagtgatgat cttggttgag atcacggagc cttgcgccgc
     1081 gaattcgtcg tgaagtgcgt cctctgtgat ggagaaggga aggtttccta caaaaagttt
     1141 catactatgg tccttaaatc aaattacact tttacttttc atttcactgc aaatcgaact
     1201 gaaaagactt gtgaggactt aaagacgaga atggggagag aagaaaggct ggtgttgaag
     1261 ccaatagaat cctgaggtcg cgactgtcaa caagaaattc caaatagctc aaccaccgat
     1321 ggaatatcca ccgtccacat gcagtatctg tcctgtgatg cgcaaatcgg gactgagcag
     1381 aaaagcggcc gcacgcgcca aatcttccgg cctcgcgttg ccgagaggcg aaagactgtc
     1441 gcacgaagca actgcttctg cgagacctgg aattgctgcg cctgctttgc tcactgcgta
     1501 gggagaaaac cgaattgcat tgacgcgaat tccgcgcaaa cccagttctt gtgccatctc
     1561 cagtgtgatt ctttccagtg cagactttgc cactccgatg ttcttgtatg gatgcgtcat
     1621 tacgcgactt gcacccaggt agctgagcga aaggactgac gcacccggcg cgagcacttc
     1681 gcattcgatc aagcttcgcg tcagcgcaat caaggaataa gcagaaacgt ccatcgcgcc
     1741 tgcgaactct tctctggtca catccagcag gttcttcgcg aatccgcctc gtatggtttt
     1801 atccatagct acagcatgaa cgatcccgtc gagtttggtg cctttgcgac ggaggtgctc
     1861 ggcaagttcc gcgacagacc cgtcaagcgt aacatcgagc acggccgttt gcacatctgg
     1921 accgagctcc tgcgcaaccg tgtccgtgaa gtcttcgtag
//
LOCUS       DAOQJL010000668         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_48107_length_1956_cov_4.135718, whole genome shotgun sequence.
ACCESSION   DAOQJL010000668 DAOQJL010000000
VERSION     DAOQJL010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48107_length_1956_cov_4.135718"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="raiA"
                     /locus_tag="PLG78_19465"
     CDS             <1..336
                     /gene="raiA"
                     /locus_tag="PLG78_19465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNE25362.1"
                     /translation="SAELKEQKMEITYHFHHLESSDGLKDYAKKKIEKLAAHFNAFHS
                     AMVRFRVETKIEHSVELTLNGDGVQFIATEKGADMYAIIDALEEKLGKQIRKHKEKHL
                     GASHRGERG"
     gene            344..1288
                     /gene="hprK"
                     /locus_tag="PLG78_19470"
     CDS             344..1288
                     /gene="hprK"
                     /locus_tag="PLG78_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNE25363.1"
                     /translation="MKQIPVASLIGAESGLELEAVSGKAGLSRTISSFDINRPGLALA
                     GFYKNFADDRIQVFGKGEFAFIEECAVADQAEIAKNFFRFELPGLVLTHGNQPPICFV
                     EMSEKHSIPILTTRQSTHSFIMQFTHWMIEELAPSTSVHGVLVEVFGVGILLMGQSGI
                     GKSETALELVERGHRLVADDAVHVRCVEGSDLYGQASELLQYHMEIRGLGIINVKDLF
                     GVGAIRGRKRIELVIELEEWNAEKEYERLGLDDELVEILGVPVPRITVPVRPGRNIPV
                     LIETAAMNHRSKKMGYHAARVLSERLKEQMEKKRYSNH"
     gene            1288..1548
                     /locus_tag="PLG78_19475"
     CDS             1288..1548
                     /locus_tag="PLG78_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNE25364.1"
                     /translation="MEKRNITVTSREGMHTRPAAVFVREAAKFKSEVMLTSGDTTVNG
                     KSIMGILMLALTSGTTVTLQVDGPDESSAADALARVLSGDSG"
     gene            1545..>1956
                     /locus_tag="PLG78_19480"
     CDS             1545..>1956
                     /locus_tag="PLG78_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE25365.1"
                     /translation="MTTTPWQTEERRIYLRDIYFLIFIVILSIGLAELFLVGEDAEPV
                     DRILSYLMVFVPLGAINLLAHYYYRNRRIRATGTLRTSLRYRLSLAFMVVAIIPSIPI
                     FLISSNVAEHLVGAFFEVDVRDAIDRSQKIIRETH"
BASE COUNT          522 a          450 c          520 g          464 t
ORIGIN      
        1 agcgcggagc tgaaggagca gaaaatggaa attacctatc actttcacca cctcgagagc
       61 tcggacgggc tcaaggacta cgcaaagaaa aaaatcgaga aacttgcggc gcacttcaat
      121 gcatttcatt cagcaatggt tcgttttcgt gtcgaaacca agattgagca ttccgtcgaa
      181 ttaactttga acggtgacgg agtgcagttc attgcaacag aaaagggcgc ggacatgtat
      241 gcgatcattg acgcactgga agaaaaactc ggaaaacaaa ttcgcaagca caaagagaag
      301 cacctgggcg catcgcatag aggcgaaaga ggctgattct cgtttgaagc aaattccagt
      361 agcatccctg attggcgcgg agagcggcct cgagctcgaa gcagtttccg gtaaggccgg
      421 cctcagccga accatttcca gttttgacat caatcggccg ggccttgctc tggccggatt
      481 ctacaagaat tttgcagatg atcgtattca ggtttttggc aaaggcgagt ttgctttcat
      541 agaagaatgc gcggttgcag accaggcgga gatagcaaag aacttctttc gcttcgaact
      601 cccggggctg gtcctgacgc acggcaatca accgccgatt tgcttcgtag aaatgtccga
      661 gaaacattct atccccatac tgactacgag acagagcact cactctttta tcatgcaatt
      721 cactcattgg atgatcgagg aacttgcgcc ttctacttcc gtgcatggag tgcttgtgga
      781 agtctttgga gtgggcatct tgctcatggg acagagcgga attggaaaat cggaaacggc
      841 gctcgaactc gtggaaagag gccatagact ggttgcagac gacgcggttc atgtgagatg
      901 cgtggaaggt tcggatcttt acggacaggc aagcgagctt ctgcaatacc acatggagat
      961 tcgggggctg ggaatcatca acgtgaagga tttgtttggc gttggagcga ttcgaggaag
     1021 aaagagaatc gagcttgtga ttgaactcga ggaatggaac gccgaaaaag aatacgaacg
     1081 tctgggtctc gacgacgagc ttgtggaaat cctgggagtt cccgtaccac gcatcactgt
     1141 gcctgtcaga cccgggcgaa acattccagt gctgattgaa acggcagcca tgaaccatag
     1201 atccaagaag atgggttacc atgccgcgcg ggttctttct gaaagactca aagaacaaat
     1261 ggaaaagaaa agatattcga atcactgatg gaaaagagaa atattactgt aacgagtcga
     1321 gaagggatgc acacgcgtcc cgcagctgtg tttgttcgtg aagccgcaaa attcaaaagt
     1381 gaagtcatgc tcacgagcgg agatactacc gtgaatggaa aaagcatcat gggaattttg
     1441 atgcttgctt tgacttcagg gactacggtc actctccagg tggacggccc agatgaatcg
     1501 tcggccgcag acgctcttgc ccgtgtgctt tcgggcgaca gcggatgacc acaacgccct
     1561 ggcagacaga agagcgcaga atctatctgc gcgacattta ctttctcata ttcattgtca
     1621 ttttgagtat tggcctggcc gaactgtttt tggtcgggga ggatgcagaa ccagtagacc
     1681 gcatcctatc ttatttgatg gttttcgttc cacttggagc tatcaattta cttgcgcatt
     1741 attattatcg caacagacga attcgcgcca ccggtactct ccgcacaagc cttcgctatc
     1801 gcttgagcct ggccttcatg gtagtggcca tcattccttc tattccaatc tttctgattt
     1861 cgtctaacgt agcggaacac ctggttggtg cattctttga agtggacgtg agggatgcga
     1921 tcgatcgctc tcagaaaatc attcgagaaa ctcaca
//
LOCUS       DAOQJL010000669         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_48629_length_1945_cov_4.687302, whole genome shotgun sequence.
ACCESSION   DAOQJL010000669 DAOQJL010000000
VERSION     DAOQJL010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48629_length_1945_cov_4.687302"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1945
                     /gene="pheT"
                     /locus_tag="PLG78_19485"
     CDS             <1..>1945
                     /gene="pheT"
                     /locus_tag="PLG78_19485"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNE25366.1"
                     /translation="PFAPVGATLPGKDGKGMTIGKANIRGVESHGMLASAAEMGLDRI
                     FAGEGLLELTDIPGALAGHSLTSIFPADLVITIDNKSITNRPDLWCHFGFAREIAAIY
                     KKKIKFHPLDRKLTFKPSKLSKKILVEKGSALAYFGAVCEGTRVLETPVWMKLRLGAV
                     GQKSINNIVDASNYAMLELAQPNHTFDLNTLKQNTVTVALSDSKSPDFKTLDGESRKL
                     PPGTVLIFDGDKKAGKSPLAVALGGIMGGEHSAIQPDTGTIFIESATFPRELIRRTLK
                     KIDLRTDSAMRFEKGQDPAKAKPTIYRIAELLRETCPDLKLGPVTGQSVLPKKSKIKV
                     TTSFLQARLGFAVTSKEISTTLTWLGYDVKSDKTGKTFQITAPTYRSQYDITIPEDIV
                     EELGRVHGYDHIAPVPPVVPVLARSLDHARLLERRMKRLLADAGFSETLNYSFVKEED
                     NLLFGKQGLALLNPLAGLSHMRLSLIPGVLHHLAANQDRYAEVRLFEFGRVYDPTAKS
                     SAFLHSAGPAHEEKRICVAMITDKIDASAQEESFAWFRETVREMAGASARQLDTALPA
                     KKDSWGIASSVSSLFHPSCQVVLKCDGEAVGFIGMLHPVLAERFGIRRTVLVAEISFD
                     LIAGEKKRASYKPPSVYPDSVFEV"
BASE COUNT          494 a          491 c          480 g          480 t
ORIGIN      
        1 ttccgtttgc tcctgtgggt gctactcttc ctggaaaaga cggaaaagga atgaccattg
       61 gaaaagcaaa catccgcgga gtggagtcgc acgggatgct tgcaagtgcg gccgaaatgg
      121 gactggatcg gatttttgca ggagaaggcc tccttgaact gactgacatt ccaggggctc
      181 ttgccgggca ttcgcttact tctatttttc cggcggacct tgtcatcacc atcgacaaca
      241 aatccattac gaatcgcccg gatctgtggt gccattttgg atttgctcgc gaaatcgcag
      301 ccatctataa gaagaagatc aagtttcatc cactggatcg aaaactcacc ttcaagcctt
      361 ctaagctttc gaagaaaatc ctggtggaga agggatctgc tctcgcttat tttggggccg
      421 tgtgcgaagg cacacgcgtc cttgaaactc cggtatggat gaagcttcga cttggagcgg
      481 tcggacaaaa atcaatcaac aacattgtag acgcttcgaa ctatgcgatg cttgaactcg
      541 cacagccaaa ccacacattc gatctaaaca ctctcaaaca gaacacggtt actgttgcac
      601 tttccgattc aaaatcaccc gacttcaaaa cactcgatgg agaatcgcgc aaacttccgc
      661 ccggcacagt tctcatcttt gacggtgata agaaagcagg aaagtcacct ctcgcagtgg
      721 ctcttggtgg aatcatggga ggggaacatt cagcgattca acccgacaca ggaaccatct
      781 tcatagaatc tgctactttt ccgcgagagc tcatccgtcg cactctcaag aaaattgatc
      841 ttcgcacaga ttctgccatg cgatttgaga agggccagga cccggcgaaa gcaaagccta
      901 ctatatacag aatcgcagaa ctactgcgcg aaacctgtcc cgatttgaaa ctgggccctg
      961 ttaccggaca gagtgtcctt cccaaaaaga gcaagatcaa agtgaccact tcctttcttc
     1021 aggctagact tggatttgca gtcacttcaa aagagatttc aacaactctc acatggctcg
     1081 ggtatgatgt gaaatcggac aagactggaa agacttttca gatcacagcg cccacgtatc
     1141 gctctcagta cgacatcacc atcccggaag atatcgtgga agaattggga agggtgcacg
     1201 gctatgatca tatagcacct gtgccgcctg tcgtgcctgt tcttgcgcga tctctcgatc
     1261 atgcgcggct tctcgaacgt cgaatgaaga ggctccttgc agacgctggt ttcagtgaaa
     1321 cattgaatta ctcttttgtg aaagaggaag acaacttgct ttttgggaag cagggccttg
     1381 cactcctcaa tcctctcgca ggcctcagtc acatgcggct cagtttgatt ccaggagtgc
     1441 tccatcatct ggcggcgaat caggaccgtt atgcggaagt gagactcttt gaattcgggc
     1501 gagtatatga cccgaccgcc aaaagctcag cgttcttgca ttctgccggg ccggcccatg
     1561 aagaaaagcg aatttgcgtg gccatgatta cggataagat agatgcatcc gcgcaggaag
     1621 aatcctttgc atggtttcgc gagactgtgc gcgaaatggc cggagcatcg gcgcgccaat
     1681 tggacactgc attgcccgct aaaaaagatt cctggggaat cgcttcttcc gtttcgtctc
     1741 tgtttcatcc ttcctgccag gtagttttga aatgcgatgg ggaggcagtg ggattcattg
     1801 gaatgcttca tcctgtgctg gccgaacggt ttggaattcg ccggaccgtg cttgtagctg
     1861 agatttcttt tgatttaatc gctggcgaaa agaagagagc ttcttacaaa ccaccctccg
     1921 tatatcctga ctctgttttc gaagt
//
LOCUS       DAOQJL010000670         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49253_length_1932_cov_3.461907, whole genome shotgun sequence.
ACCESSION   DAOQJL010000670 DAOQJL010000000
VERSION     DAOQJL010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49253_length_1932_cov_3.461907"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..986)
                     /gene="mtnB"
                     /locus_tag="PLG78_19490"
     CDS             complement(279..986)
                     /gene="mtnB"
                     /locus_tag="PLG78_19490"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNE25367.1"
                     /translation="MAIGFQTDRVSEINPIQTRDTSPRDLICEICRNFYTLGWVSGTG
                     GGVSIRRNDTIYMAPSGVEKEKIKPEDIFELDLAGNVTRHAKNEKLKLTECAPLFLSA
                     FEMRNAGAVLHSHSPNVVLSTLYAKRVEGRISVLEFTELEMIKGLEGHGYHDVFTLPV
                     VDNTAKECDLTDSLREAIAKYPKTCAVAVRNHGIYVWGKDEVKAKTQAECIDYLCEIL
                     VRMRQSGITRDQHPMGV"
     gene            1086..1712
                     /locus_tag="PLG78_19495"
     CDS             1086..1712
                     /locus_tag="PLG78_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25368.1"
                     /translation="MHNTEATAQALNILRRGDQFQWSVIPFLAFVCYVYFTEIKNRNW
                     ASVSAGLALYGVHWFYEILNALIQHATGHALWTVPTGTSFLIFIGVGIEISLMFAVSG
                     IIISRLLPENPSEKIAGLPLKWLHVISFAVLYSVIEIFLVKATPVFVWVYDWWGAIPV
                     GLTVYVPFFAAAVFAYYWQPRTRLIFISAVWILDLFLVLVFAVVLRWI"
     gene            complement(1739..>1932)
                     /locus_tag="PLG78_19500"
     CDS             complement(1739..>1932)
                     /locus_tag="PLG78_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25369.1"
                     /translation="HGGSIIMGILGLGFYLTNITGAAASAGRFNTFQERKFQQTIRDR
                     FFNIDFVEQTSDIRFETSF"
BASE COUNT          444 a          465 c          482 g          541 t
ORIGIN      
        1 gagagaggat caggaaagaa catgatcgca taacgggtct cacagtggcg atttctccac
       61 gcaggttgtt ctgcactctt tcttaatgga agtgcaagca aggaatgcat agaccccggt
      121 ctggtctgca cagctagagg aacatgtgtt tcttgcgagt gcctgctgga ggatgacgca
      181 ttgttggaag ttatcttcgc agtttcctac gcactcagaa tgggacatgc aaccggagac
      241 gagcaggaat ccagccagga tcacaatcgc agatttcatc agacacccat gggatgctgg
      301 tctcgtgtga tgccgctttg acgcatgcga accaggatct cgcacaaata atcgatgcac
      361 tctgcctgag ttttggcttt cacttcgtct ttgccccaga catagatccc gtgatttcgc
      421 acagccacag cacaggtctt tggatacttt gcgattgctt ctctcagaga atccgtgagg
      481 tcacattcct ttgcagtgtt gtctacgacc gggagagtaa atacatcgtg gtaaccgtga
      541 ccttccaggc ctttgatcat ctccagttcc gtgaactcga gaactgaaat tcgtccttcc
      601 actctttttg catacagtgt ggacagcact acgtttggcg aatgcgaatg aagcaccgcg
      661 cctgcgtttc gcatttcaaa agcagaaagg aatagaggcg cgcattcagt gagtttgagt
      721 ttttcgtttt ttgcgtggcg cgttacattt ccagccagat ccagttcaaa gatatcttcc
      781 ggtttgattt tttctttttc aacgccactg ggcgccatgt agattgtatc attgcgtcga
      841 atggaaaccc caccgcccgt tccactgacc cagccaagag tgtaaaaatt gcggcagatc
      901 tcgcagatca aatctctcgg ggaagtatct ctggtctgga tggggttgat ttcgctgacg
      961 cgatctgtct gaaatccaat ggccatggcg accagataaa gggaagtgag gctgttcggc
     1021 cagatgaaaa aatctccctg gatcgatatt gcgcggaaac gcagcgcgca ccctgatggc
     1081 attctatgca caacacggaa gcaacagcgc aggcgttgaa tattcttcgg cgcggtgatc
     1141 agtttcaatg gagcgtcatc ccgtttctgg catttgtatg ctatgtttat ttcactgaga
     1201 tcaagaatcg aaactgggct tccgtctcgg ctggtcttgc cctgtacgga gtgcactggt
     1261 tttacgaaat cctaaatgct ctcatccagc atgcaactgg acatgctctc tggactgtgc
     1321 cgacaggtac ttcgtttttg attttcattg gagtcgggat tgaaatctct ttgatgttcg
     1381 ctgtttccgg gatcatcatt tccaggctcc ttccagaaaa cccgtctgag aaaatagccg
     1441 gccttccact caaatggttg cacgtgattt cttttgccgt actctattca gtcattgaaa
     1501 tcttcctggt caaggccacc cccgtatttg tctgggtgta tgactggtgg ggcgcgatcc
     1561 cggttgggct tactgtatat gtgccatttt ttgcagcggc agtattcgcg tactactggc
     1621 agccccgcac aagactgatc ttcatcagcg cagtgtggat tctggatttg ttcctggttc
     1681 ttgttttcgc agtagtcctt cgctggatct agtggtgcgc actcaatgac gatttaaatc
     1741 agaaactggt ttcgaaccga atgtctgagg tttgttccac gaaatcgata ttgaagaacc
     1801 tgtcacgaat cgtttgttga aatttgcgtt cctgaaaagt gttgaatcta cctgcggaag
     1861 cagcagcgcc tgtgatgttg gttagataga aacccagacc aagaattccc atgatgatgg
     1921 aaccgccatg tc
//
LOCUS       DAOQJL010000671         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49430_length_1928_cov_4.096103, whole genome shotgun sequence.
ACCESSION   DAOQJL010000671 DAOQJL010000000
VERSION     DAOQJL010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49430_length_1928_cov_4.096103"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLG78_19505"
     CDS             complement(<1..302)
                     /locus_tag="PLG78_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE25370.1"
                     /translation="MGKYFFALILAVLSCQKHVEAAEDISFEAMATGFAARFEVPEIQ
                     ASDFLSRSSSYTLLDARTPEEQSVSRIPGAILVRPDTDPASIPHRPGEKLVVYCAGG"
     gene            370..1167
                     /locus_tag="PLG78_19510"
     CDS             370..1167
                     /locus_tag="PLG78_19510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE25371.1"
                     /translation="MIRKKLTIVLFAALVSTALHAESIDVAVDNVFIQTFRGKPGIWT
                     VPGKDSFEYLLKQYGSTKEEVLSLNGGKFTSQSYMFFPYSEAVLKQVLADGKGRRIQK
                     VDPTRLMWPIETPVYTSRYGNRGGERHPGLDFGCARNTVVVAADTGVVVRAGWMGGLG
                     LAVAIQHANGNVTWYAHNTAVLLSEGEHVNRGQIVAYSGSTGRSTGPHVHFEVRFMDV
                     PLDPEDFIQFGLVRPDLVVREGPQQALSQEVSLDNVVMNPGQSRPGP"
     gene            1203..>1928
                     /locus_tag="PLG78_19515"
     CDS             1203..>1928
                     /locus_tag="PLG78_19515"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNE25372.1"
                     /translation="MLAGPPGLACFDFDNTLIQGDLGESVMYYILMQGLIAGDLPEFW
                     QQLNHPCIPHEDVETWKRLWAIYKDQEDAFSYEKIAHGLIEAYESIAEKSGLEAAYRW
                     TCTIFCGSSEEELAGIAKHVFEENQLPGLAPLSLGAREIDNSIRVRRTFQNLTARLMK
                     AGWEVRVITASPECTIRYAANKAFGIDEQNVRGMRLAISNGILMPQIIEPMTFDSGKV
                     KALREITDRPIDFAAGDSFTDLAM"
BASE COUNT          472 a          509 c          497 g          450 t
ORIGIN      
        1 cccccggcgc agtaaacgac cagtttctcc cccgggcgat gggggataga ggcagggtct
       61 gtgtccggcc gcaccagaat cgcccctgga atcctggaga cgctctgctc ctctggtgtg
      121 cgggcgtcca ataaggtgta ggaactacta cgcgatagaa aatcggacgc ctggatctct
      181 ggaacctcga atctggcggc aaacccggtt gccattgctt caaaacttat gtcttctgct
      241 gcttccacgt gcttctgaca gctcagaacg gccagaatca gggcaaaaaa gtacttgccc
      301 atggccttcc ctggctgcga ctgagtaccc agtctgccga tattaggttc tatccaccgg
      361 ttttttgcat tgattagaaa aaaactcacc attgtgctct ttgcagccct cgtttccacc
      421 gctttgcacg ctgaatccat cgatgtggcg gtagacaacg tattcattca gaccttccgc
      481 ggcaagccag gcatctggac agtcccgggc aaggactcct tcgagtatct gctcaaacaa
      541 tacggttcta ctaaagaaga agtattgagc ctcaatggcg gcaagttcac atctcaatct
      601 tacatgttct ttccatattc tgaagcagtt ctcaaacagg ttctggcaga tggcaaaggt
      661 agacgcatcc agaaagtaga tcctactcgc ttgatgtggc ccatcgaaac tccagtgtac
      721 acttcccgct acggaaatcg cggcggggaa agacatcccg gactggattt cggatgcgca
      781 cgcaacacgg tcgttgtggc tgcagatacc ggagtcgtgg tacgggcagg atggatgggc
      841 ggacttggac tggcggtcgc cattcagcat gcaaacggga atgttacctg gtatgcgcac
      901 aacacagcgg tccttctttc agaaggagag catgtcaatc gcgggcagat cgttgcctat
      961 tcgggttcaa caggccgctc tacaggtcct catgttcatt ttgaagttcg cttcatggac
     1021 gttccactcg atccggaaga tttcatccag tttggactcg ttcgccctga tctcgttgtt
     1081 cgcgaagggc cacagcaagc gctctctcag gaagtgagcc tggacaacgt agtaatgaat
     1141 cctggccaga gccgacccgg cccttgatcc tgactcaagc aaacttagat tcgctcgaag
     1201 aaatgctcgc aggtcccccg ggactcgcat gctttgattt cgataacact ctcatacagg
     1261 gagacctggg cgaatctgtt atgtactaca tactgatgca gggtttgatc gcgggagatc
     1321 ttcccgaatt ctggcaacaa ctgaaccatc cctgcattcc gcacgaagat gtggagacct
     1381 ggaaaagact ctgggcgatt tacaaagacc aggaagacgc tttcagttat gaaaagatcg
     1441 ctcatggcct catagaagca tacgaatcga ttgcagaaaa atccggtctg gaagccgcct
     1501 atcgttggac ttgcacgatc ttctgtggca gctccgaaga agaactcgcg ggcattgcaa
     1561 agcatgtgtt tgaagaaaac cagttacctg gactcgctcc gctcagtctg ggtgcgcgcg
     1621 aaatagacaa cagcattcga gtaagaagaa cgttccagaa tctgactgct cgcctcatga
     1681 aggcaggctg ggaagttcgc gtgatcactg cttctcccga atgcaccatc cgttatgcgg
     1741 caaataaagc ttttggaatc gatgaacaaa atgtacgcgg catgcgtctt gccatttcga
     1801 atggaattct aatgcctcag atcatcgagc ctatgacttt cgacagcggg aaagtcaaag
     1861 cgctcagaga gatcacagat cggcccattg attttgcagc tggagattcg ttcacggacc
     1921 tggccatg
//
LOCUS       DAOQJL010000672         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49484_length_1927_cov_4.419872, whole genome shotgun sequence.
ACCESSION   DAOQJL010000672 DAOQJL010000000
VERSION     DAOQJL010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49484_length_1927_cov_4.419872"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLG78_19520"
     CDS             <1..398
                     /locus_tag="PLG78_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25373.1"
                     /translation="KFLRDLAAKKKDLGTFLSSWVQTVTRAARSGKFVGCPLAAFAYQ
                     LSEAEAKVYGDRLALTTHRWLGDVVQLIESLKKAGSLSATLDAEALGMRILTAYEGAV
                     TMWRLTRSDHHWTHLKQEFARIARDAVET"
     gene            563..1825
                     /locus_tag="PLG78_19525"
     CDS             563..1825
                     /locus_tag="PLG78_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25374.1"
                     /translation="MKRILGILALFALVTPAFAQTSLSSLFQKPGAIGDSLSQGFFGV
                     TVEQKTQDWAYPVLVSKQAGSNLSYNVLKGPYVNLEDVLKGDCGPICIASAIIGGNGS
                     TVALPTHAGITGADYTDALYKSGQCQDIRATKQVKEWYWATWYWYTYRWITVADCQEP
                     DKFFRFGLRDAGTQIQIMEKVRPTFAFAAVGANHVLCTALGTSLACLDEPRFKRDVAE
                     SFRRLKAMGSLKGGVIFSVPDVTTIAYLERWNDPSGRANYSGLKPFYRGTVSSPDQVL
                     DAGEIATVQKFLAMENTELRNQATGANFAFTDAKALFDDIKANGRVLKSSSGYSPGNG
                     QAMWPLPGRPGLFGLDGVHPNRYGHSIMANELILAINKQYGVAIPKVDEYSAWYYDSL
                     NRSPVDLKNFLTNSIFGQVITWIVDTFM"
BASE COUNT          476 a          470 c          498 g          483 t
ORIGIN      
        1 acaaattcct gagagatctg gccgccaaga aaaaggatct gggaactttc ctcagtagtt
       61 gggtccaaac tgtgaccagg gctgcccggt ctggaaagtt tgttggatgt cccctggccg
      121 cgttcgctta tcagctttcc gaggcagagg cgaaagtgta cggggacagg ctcgctctca
      181 cgactcatcg ttggctcgga gatgtagtcc agctgattga atctttgaag aaggcaggca
      241 gcctttcggc cactctggat gcggaggctc tgggaatgcg aatcctgact gcttatgagg
      301 gagctgtgac catgtggaga ctgaccagat cggaccacca ctggactcat ttgaagcagg
      361 agttcgctcg gatcgcacgc gatgccgtgg agacataatc ggtttaacgt gaaacggcga
      421 ataacgttgg aatttcgcat ttcagtctta tgtctcttat gatttcattt tttgacacag
      481 tgtcattgaa aaagaattga tgatccggat aggggatgct agaagtgggc atccgatccg
      541 ttggatcgtg tggggggaaa atttgaaaag aatcctgggc attcttgcgc tctttgcgct
      601 ggtcacacca gccttcgcgc aaacatcatt gtcatcgctc tttcagaagc caggagccat
      661 tggagactct ctgtctcaag gtttcttcgg tgtaactgta gagcaaaaaa ctcaagactg
      721 ggcctatcca gttctcgttt ctaagcaggc aggctccaat ctgtcttaca acgttctgaa
      781 aggtccttat gtaaacctgg aagacgtttt gaaaggggat tgcggtccca tctgcatcgc
      841 atctgcgatc ataggtggaa acggaagcac tgtcgctctc ccgactcatg ccgggatcac
      901 aggagcagac tacacagacg ctctctataa aagcggtcag tgccaggaca tccgtgctac
      961 caagcaggta aaagaatggt actgggccac ctggtactgg tacacatatc gctggatcac
     1021 agtggcggat tgccaggaac cagacaagtt cttccgcttt ggtctgcgcg atgcaggcac
     1081 tcaaatccag attatggaaa aagtgcgtcc tacattcgca ttcgcggcag tgggcgctaa
     1141 ccacgtactc tgtactgccc tcgggacaag ccttgcttgt ctggacgagc cacgcttcaa
     1201 gcgcgacgtt gcagaatcat ttagacgcct caaggcgatg ggttctctga aaggtggagt
     1261 gattttctct gttccagacg tgactacaat tgcgtacctc gaaagatgga atgatccttc
     1321 cggccgcgcg aattactccg gtctcaaacc tttctatcgc ggaactgttt ccagtccgga
     1381 tcaggttctt gacgcaggag aaatcgcgac tgttcagaag ttccttgcta tggaaaacac
     1441 agagcttcgc aatcaggcaa ctggtgcgaa tttcgcattc acggatgcga aggctctctt
     1501 cgacgacatc aaagcaaatg gtcgggtgct caaaagctca agcggctact ctccaggaaa
     1561 cggacaggcc atgtggccac ttcctggtag accaggtctc tttggtctag atggagttca
     1621 tccaaatcgt tacggtcatt ccattatggc aaacgagttg attctcgcga tcaacaagca
     1681 atacggagtg gcaattccta aagtggatga atactctgca tggtactacg attctctcaa
     1741 tcgcagccct gtggatctca agaacttcct tacgaattct atcttcggtc aggtgattac
     1801 ctggatcgta gacacattca tgtgatctga aaattgatca cagaaaaacc cggggccaac
     1861 gctccgggtt ttttttcgct cttgtcaaca aagcactgtt ccttccaatg aggtaatatg
     1921 aatcgca
//
LOCUS       DAOQJL010000673         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49543_length_1926_cov_4.437734, whole genome shotgun sequence.
ACCESSION   DAOQJL010000673 DAOQJL010000000
VERSION     DAOQJL010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49543_length_1926_cov_4.437734"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLG78_19530"
     CDS             <1..835
                     /locus_tag="PLG78_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNE25375.1"
                     /translation="DAALTFEPETSNALGFGYRVGYLGLLHMEIVQERLEREFNLALI
                     NSAPSVKYRITFTNGTVEEIDNPANWPDPQRIEKMEEPYVHANVLTPPEYVGNILALF
                     QDKRGIQENMIYLADGKVQLIYAMPLSELIFEFYDKLKSCSRGYASLDYEPHEYRESD
                     LVKVDILVNAEPVDALAMIVHRGRADMRGRHLVDKLKELIPRQQFQIPLQAAVGAKVI
                     ARENISALRKNVTAKCYGGDITRKKKLLEKQKEGKKRMKQIGSVEIPQEAFLALLKTD
                     E"
     gene            843..1721
                     /locus_tag="PLG78_19535"
     CDS             843..1721
                     /locus_tag="PLG78_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25376.1"
                     /translation="MLLVGLLCTLLHCRPETLLIYEIKTPEKVEEWTITPMRRGEAVH
                     AISRSTPRGKSASDLEFRVFIGKKETLEEAAMRMGAKRENGAMQVQPLRISVFKDRLP
                     GVEDVRILDFSRFVIRRNNTVIEQIAFENFQKCCAHPALIDYRWFFGAKYQGQLELEL
                     PDYMREMEEDTASPEEKEQAQKKKIAEWKPWAPSRIRSGDRLAAWLIFPPLEIETDYI
                     LSHRDPGVMPDLPFRITMVRRNREILDTWTPEASERAISAEEREILQHEQEDTFIMHR
                     QLQKHAELRDKEQKKN"
BASE COUNT          551 a          492 c          465 g          418 t
ORIGIN      
        1 tgacgcggca ctcacgttcg aaccggagac atcaaacgcc ctgggcttcg gttatcgcgt
       61 tggatatctg ggccttctcc atatggagat cgttcaagaa cgcctcgaaa gagaattcaa
      121 tctggcgcta atcaactctg ctccttccgt taaatatcgc ataacgttta caaatggtac
      181 agttgaggaa atagacaatc cagcaaactg gcccgatccg cagcgaattg aaaaaatgga
      241 agaaccttac gtgcacgcaa atgtgctcac tcctccagaa tacgttggaa atattctagc
      301 actatttcag gacaagcgcg gcattcagga aaacatgatc tatctggcag acggcaaagt
      361 acaactcatc tacgccatgc ctctttctga attgatcttt gaattctacg ataagctaaa
      421 atcttgttcg cgcggctatg cttcattgga ctacgaaccg cacgaataca gggaatcaga
      481 cctggtcaaa gtggacatac tggtaaacgc agagccagtg gatgctcttg ccatgattgt
      541 tcatcgaggg cgagcggata tgcgcggacg ccatcttgtg gacaagctca aagaactcat
      601 tcctcgccag cagtttcaaa ttcctcttca ggcagcagtg ggcgcaaaag tgattgcgcg
      661 cgaaaacata tctgcgctcc gtaagaacgt gactgccaaa tgctatggcg gtgacatcac
      721 gcgcaaaaag aagcttctgg aaaagcagaa ggaaggcaaa aagcgcatga aacagattgg
      781 ttctgtggag attccgcagg aggcttttct tgccctgctc aaaactgacg aatagacgtt
      841 ccttgctcct cgttggtctg ctttgcactc tcttgcattg caggccagag actcttctca
      901 tctacgaaat caaaacgccg gaaaaagtag aagagtggac catcactcca atgcgcagag
      961 gggaagcggt ccatgcgatt tccagatcaa ccccgcgagg caagagcgcc tcagaccttg
     1021 aattcagagt attcattgga aagaaagaaa ctctcgaaga agcggccatg cgcatgggtg
     1081 cgaagagaga aaacggagcg atgcaagtgc agcctcttcg catcagcgta tttaaagata
     1141 gactgccggg ggtagaagac gttcgcatcc tcgatttttc caggttcgtc attcgcagaa
     1201 acaatacagt catcgaacaa atcgcctttg aaaactttca aaagtgctgc gcccaccctg
     1261 ctttgatcga ctacagatgg ttctttggcg ccaaatacca gggccagctg gaactagaac
     1321 tcccagacta catgcgcgaa atggaagagg acaccgcctc ccccgaagaa aaagaacagg
     1381 cccaaaagaa gaagatcgca gaatggaagc cctgggcccc ctctcgcatt cgcagtggag
     1441 atcgtcttgc cgcatggttg atctttccgc ctctggaaat agaaacggac tacattctct
     1501 ctcatcgtga tcccggagtg atgcctgacc ttccatttcg cataaccatg gtaaggcgaa
     1561 acagggaaat tctggacacg tggactccgg aagcttccga aagggccatt tcagcggagg
     1621 aaagagagat attgcagcat gagcaggaag atacctttat catgcacaga cagctacaga
     1681 aacatgcaga gctaagagat aaagaacaga aaaagaattg agccgcccgc atcgcgggcg
     1741 actatccatg gttagacggg ctcgagatta cagcagaccg gaattgtcca ctacacccat
     1801 cagttctttg actgctgccg ccgatttgtc cagggccgcc ttctcttctg cagtcagttt
     1861 gatttcaatg attcgctcaa atccacctga gcccaccaca accggcacgc ccatgaagat
     1921 tccatt
//
LOCUS       DAOQJL010000674         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49547_length_1926_cov_3.858365, whole genome shotgun sequence.
ACCESSION   DAOQJL010000674 DAOQJL010000000
VERSION     DAOQJL010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49547_length_1926_cov_3.858365"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLG78_19540"
     CDS             complement(<1..556)
                     /locus_tag="PLG78_19540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE25377.1"
                     /translation="MKNRKIVIIGGVAGGATAAARARRVDEHAEITLVEMSGYVSYAN
                     CGLPYYIGGDIKERSKLLLQTPKGFHDRYRVRVLTHTEALKIDREKKEVLVRGPDGEQ
                     TLSYDSLILSQGGSPVVPPLPGIPSQHAFTLRNVEDTDAIHDFVEQAKPRTAVIAGGG
                     FIGLEMAEALHSRGLSVQVVEKFNQ"
     gene            complement(581..802)
                     /locus_tag="PLG78_19545"
     CDS             complement(581..802)
                     /locus_tag="PLG78_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE25378.1"
                     /translation="MYCAQTGSWYLERIIWLVAGVFTSVGTALAFFVSPWWLILPALV
                     GVNLIIFSLTGFCIMANILVMLGAKSRVA"
     gene            930..>1926
                     /locus_tag="PLG78_19550"
     CDS             930..>1926
                     /locus_tag="PLG78_19550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE25379.1"
                     /translation="MKQIWLPLAILWSIVAFPLRAEERLTLGEIWASVHGKSNAQKAA
                     ALELQASSIEKDRREWHWAPRIYAEGRAFGTNDPVMSFSSMLQTRNLKSADLSVPSPL
                     LSPQAYLNPLSVLAPDTLNHPVSSGFYRGILGIELPVYEGGSGQARVDAAGSAAKARE
                     WELQATVKQEYAAVAQAYAEILIRDKEDADLGILNQTLTAILSRYQLGSAANPVGRSG
                     YLGMQTLRNRIQTTLANNKMRKNAARSLVEALANEIPADWNIQSEEITPFADRYMALS
                     DTTGDSERVRAMDAMADSIAYKEVFEQAKLLPQAGVFGEASATGGRRGVGDAYYAG"
BASE COUNT          491 a          530 c          466 g          439 t
ORIGIN      
        1 actgattgaa tttctccacg acttgaacgg atagccctcg cgaatgcaaa gcctccgcca
       61 tttcaagacc aatgaatccg cccccggcga tgactgcagt ccggggcttt gcttgttcca
      121 caaagtcatg aatcgcatct gtgtcttcta cgttgcgcaa tgtaaacgca tgctgcgaag
      181 gaatcccggg aagaggagga actacaggac ttccgccctg cgaaagaatg agagaatcgt
      241 aggagagtgt ttgttctccg tcgggtccgc gaacaagcac ttcttttttt tcacgatcta
      301 tcttgagagc ctccgtatga gtgagcactc gcacacgata tcgatcatga aagcctttgg
      361 gcgtttggag aagtagcttg cttcgctctt tgatatcgcc tccaatatag taaggcaatc
      421 cacagttcgc gtaactcaca tacccgctca tttctacaag agtgatttca gcgtgttcat
      481 ccactcgtct cgctcgcgca gccgcggttg cccctcccgc aactccgcca atgatcacaa
      541 tctttctatt cttcatggat ccttcctact gagcgttctg ttaggcgacg cggctcttcg
      601 caccaagcat cacgagaatg tttgccatga tgcaaaaacc agtcagagaa aaaatgatca
      661 gattcacacc taccagtgca gggagaatca accaccatgg gcttacaaaa aatgccaggg
      721 ctgtgcctac acttgtaaaa acacccgcca caagccagat aatccgttcc agataccagc
      781 ttcctgtctg tgcacaatac atactcatcc gtcctcgcag ggttgtgcag aatggagacc
      841 cattcctttc actcgtcttt cgcgacatat accctacctg gtatagtata agagtggacg
      901 acggtacgtc aacgcatatt cttgcctcca tgaaacagat ctggcttccc ctcgcgatcc
      961 tgtggagcat cgttgccttc ccactgagag ctgaagaacg actcacatta ggcgaaatct
     1021 gggcttccgt tcacggtaag tccaatgctc agaaagcggc cgcccttgag ttgcaggcgt
     1081 cttctataga aaaagaccgc agagaatggc actgggcccc gcgaatctac gcagaagggc
     1141 gcgcgtttgg aacaaacgat ccggtgatgt ctttctcttc catgttgcaa acacggaatc
     1201 tgaaatcggc agatctaagt gttccctctc cactcctcag tccgcaggcg tatctaaatc
     1261 ctctctcagt cctcgcgccg gacactctga atcatcctgt ttcatctgga ttctatcgcg
     1321 ggatcctggg cattgagctc cccgtgtacg aaggcggttc aggacaggca cgtgttgatg
     1381 cagcgggcag tgcggcgaaa gctcgcgaat gggaactcca ggcaactgtg aagcaggagt
     1441 atgcggctgt agctcaagcg tatgcggaga tcttgattcg tgacaaagaa gatgcggatc
     1501 ttggaattct aaatcaaact ctcaccgcaa tcctttcgcg ctaccagctt ggctccgccg
     1561 caaaccccgt gggtcgatcc ggctatcttg gaatgcaaac tctgcgaaac agaatccaga
     1621 caacgcttgc aaacaataaa atgagaaaga acgcagcacg ctctctcgtg gaagccctgg
     1681 caaacgaaat acccgcggac tggaacatcc agtctgaaga aatcacgccc ttcgcagacc
     1741 gttatatggc attgagcgat acaacgggcg attcagaaag agttcgtgcg atggacgcaa
     1801 tggcagactc gatcgcctat aaagaagtct ttgaacaggc aaaactactg ccgcaagccg
     1861 gagtcttcgg agaggcctct gctaccggag gaagaagagg cgtcggagat gcatattacg
     1921 ccggct
//
LOCUS       DAOQJL010000675         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_49934_length_1918_cov_3.763285, whole genome shotgun sequence.
ACCESSION   DAOQJL010000675 DAOQJL010000000
VERSION     DAOQJL010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49934_length_1918_cov_3.763285"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLG78_19555"
     CDS             complement(<1..987)
                     /locus_tag="PLG78_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25380.1"
                     /translation="MRKIQVVSLLPLAIASTLAAAFPKFSKDDFSNPSGESAFSIVRS
                     IELDDFSVASVRYLPDGQRVIAGSSDGRVALVDLASARAQWAVSPIANFRDWISVLDV
                     DPTGQKFVFIVSGDQGDAYMTLYIASTQDGRILKKISGEKSNLYQKHFDYEIDLRYPG
                     PDGEKKRLEEDNLAAYWLMVPYAARFTSKGGLITSWKNSADTHAMYDRSFRLFDSNFR
                     KVWEYQVKADEKTWDPLEPAGFKNGLPVPPIVEMTDGSFLFGTPHARVQQLTDTLAKS
                     NMKKPNLVDHAFAPVFANPKSIHSEFEDTAMPVSDLAVGADGKVYVSAGDGGQ"
     gene            complement(1074..>1918)
                     /locus_tag="PLG78_19560"
     CDS             complement(1074..>1918)
                     /locus_tag="PLG78_19560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE25381.1"
                     /translation="LAQKGQMMQSLAGVQAILDKAERYGAKDLAPQEFQSATSDLAGA
                     RTDFDGNQLKTGSIKLKQAEEQAGLALSKSIEQYAAKRKAEATVAVNGAEKEIDGIPA
                     GKEEVKTLRDYASAAREALNSATTNYGNQKYEDSIKDSDEAIRLAGIIREQALAAKGK
                     YNVADRNTNITDNKNLGKLPAGWQLYEVQKKKPEDCLSCISQRPQIYGQTRLWTRIYQ
                     ANKQVIKNPNLIYPGQKLFIPPKTGEIPALNRDEILTPAKQQPAQPQPMENKTETAPQ
                     ENMK"
BASE COUNT          451 a          479 c          464 g          524 t
ORIGIN      
        1 ttgaccgcca tctcctgctg aaacgtaaac ttttccgtcc gctcccactg cgagatcgga
       61 tacaggcatt gcagtatctt caaattcaga atgaatgctt tttggatttg caaacacggg
      121 agcgaaagcg tgatccacga gatttggttt tttcatattt gattttgcaa gagtatccgt
      181 aagctgctgc acccgcgcgt gcggagtccc aaacaaaaac gatccatccg tcatttccac
      241 aatcggagga accggaagac cattcttgaa tcctgcaggc tcaagcggat cccacgtctt
      301 ctcatctgct ttcacctggt attcccagac ttttcgaaaa ttggagtcaa acaaacgaaa
      361 tgaacgatcg tacatcgcgt gagtgtccgc gctgttcttc caggaagtga tcaatccacc
      421 cttcgaagtg aagcgtgcag catacggaac cataagccag tacgcagcca gattgtcctc
      481 ttccagacgc ttcttctctc cgtctggacc cggataacgc agatcaattt catagtcgaa
      541 atgcttctga tacagatttg atttttcgcc tgagattttc ttaagaatgc gaccgtcctg
      601 ggtagacgcg atgtagagag tcatgtacgc gtcgccttga tcgcctgaaa caatgaagac
      661 gaacttctga ccggttggat ctacatccag gacagaaatc caatctcgaa aattggcaat
      721 cggagatacc gcccactgag cgcgagcgct ggcaagatcc accagggcca ctctaccgtc
      781 agaggatcca gcaatcactc tctgtccatc cggcaagtat cgaaccgatg caacagagaa
      841 gtcatccagt tcaatggatc gaactatgct gaatgcgctt tcaccggatg ggttggaaaa
      901 gtcatccttg ctgaactttg gaaatgcagc agccagagtg gacgcgatgg ccagaggcag
      961 aagagagact acctgaatct tacgcatgcc gaaccatacc gtttgcctgg aaccaggcaa
     1021 acaaaaaagc cccggatttc cggggctttt gcctacaaaa caagaaggtt gaattacttc
     1081 atgttttcct gaggcgcagt ttctgttttg ttttccattg gctgcggctg agctggctgc
     1141 tgctttgccg gagtgagaat ttcatcacga ttgagagcag gaatttctcc agtctttggc
     1201 ggaatgaaga gtttctggcc ggggtaaatc agattcgggt tcttgatgac ctgcttgttt
     1261 gcctggtaaa tgcgagtcca gagtctggtc tgaccataga tctgcggacg ctgcgaaatg
     1321 catgagaggc aatcctccgg tttctttttc tgcacttcgt agagctgcca tccagcagga
     1381 agtttgccca gattcttgtt gtctgtgata ttcgtattgc gatctgctac gttgtatttg
     1441 cctttcgccg cgagagcttg ttcacgaatg atcccagcaa gacggattgc ttcatctgaa
     1501 tctttgatgc tgtcttcgta tttttgattt ccgtagtttg tggtcgcgga gttcagagct
     1561 tcgcgagctg cagatgcata atcgcgcagg gttttaactt cctctttgcc tgctggaata
     1621 ccatcgattt ctttttcagc gccattcaca gcaacggttg cttctgcttt gcgcttggct
     1681 gcgtactgct ctatagattt agaaagtgca agacctgcct gctcctctgc ttgtttgagc
     1741 ttgatagaac cagttttcag ctgattgcca tcaaagtcag tgcgagctcc tgcgagatca
     1801 gaagtggcag actggaattc ctgcggagcc agatcttttg cgccgtatct ttccgcttta
     1861 tccagaattg cctgcacgcc cgcgagagac tgcatcatct gacctttttg agccagag
//
LOCUS       DAOQJL010000676         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_50182_length_1913_cov_3.728741, whole genome shotgun sequence.
ACCESSION   DAOQJL010000676 DAOQJL010000000
VERSION     DAOQJL010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50182_length_1913_cov_3.728741"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLG78_19565"
     CDS             <1..253
                     /locus_tag="PLG78_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25382.1"
                     /translation="EKGDQLVINQNYYSDWRATDGFEAKSHNGIISVLLHAGKNRFSI
                     YYLSTAAVVGFLLGMAMFVGVYFFCEFRTRRFIPEQNPA"
     gene            complement(228..1757)
                     /locus_tag="PLG78_19570"
     CDS             complement(228..1757)
                     /locus_tag="PLG78_19570"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE25383.1"
                     /translation="MDDTTRRRLLGFVNISAWVTLLSSYASTAWAALLFALLVILWLA
                     IQLRRSRQSRASAESELQHAQERLSRLQHQEEMTASRLRESVLGLREFLTRLNSKVQA
                     HAAAYSDLSSVMEEFAASIDMSNSATESQIELIDQVSAQASEVELLLRSIRGAGEELQ
                     AQIEEARTGGESVTRSAQNLSQAMDDIEKAFATVSQVTRIMTEVSDRTNLLSLNASIE
                     AARSGEHGRGFAVVAEEISKLAEKSAANAKQIASVISGNASAISTGHKSANVAESKVQ
                     EQRSVFLSIAERYGQVTENVRNQIGKSEELVGRIRSLRELSSEISGAAREQKAGVDSA
                     MKSLLSMESSTSSLVSDANFIEMTAKLIEDQAQSLQSEYHEDLRKTEARLATDIRSSL
                     DRLPFDSDVDLWMEQATAVVPSAAFRLYLCNSAGTQLSPNFLRKGNAFVAQPEYRGSN
                     WSERAYFAENMSRVARSGGASMSAKYIDRETEQETITLTLQVAPDAFLFIDLKPDSAQ
                     G"
BASE COUNT          429 a          479 c          491 g          514 t
ORIGIN      
        1 cgaaaaggga gaccagcttg tcatcaatca aaattattat tctgattgga gagcgacaga
       61 cggatttgaa gcaaagtcgc ataacggtat catttccgtg cttttgcatg ccgggaaaaa
      121 taggttttcc atctactatc tgagtacggc ggcagtcgtt gggtttcttc tgggaatggc
      181 aatgtttgtg ggcgtgtact tcttctgtga gtttcgcacg agaagattca tccctgagca
      241 gaatccggct tgaggtcaat gaatagaaat gcgtctggcg ccacctggag agtgagcgtt
      301 attgtttctt gctctgtttc tctatctatg tatttcgctg acatggatgc cccgccactt
      361 ctggccacgc gtgacatgtt ttctgcgaag taggcccgct cgctccagtt ggatccccga
      421 tactctggct gcgcgacgaa tgcatttccc tttctaagaa agttgggtga caactgtgtt
      481 cccgcggagt tgcaaaggta aagacgaaac gcggccgacg gaacaacagc tgttgcttgc
      541 tccatccaga ggtcgacatc tgaatcaaat ggtagccgat caagactgct tcgtatgtct
      601 gtagccaggc gtgcttccgt ttttcttaag tcctcgtggt attcgctctg cagagactgc
      661 gcctggtctt caatgagttt agctgtcatt tcaatgaaat tcgcatcgct tacgagcgag
      721 cttgtagaag actccatgga caaaagggat ttcatcgcgg agtctacgcc ggccttttgt
      781 tctctggcag caccgctaat ttcagatgaa agctcgcgca gactacgaat acgacctaca
      841 agctcttcac ttttgccgat ttgattgcgc acgttttctg tgacctgacc gtatctttcc
      901 gcaattgaaa gaaacacgct tctctgttcc tggactttgc tttccgcaac gttagccgac
      961 ttatgcccgg tggagatagc agatgcattt ccagaaataa cggaggcgat ttgtttcgca
     1021 ttggcagcgc ttttttctgc gagcttgctg atttcttctg caacgacggc gaatcctctt
     1081 ccatgctctc ctgagcgtgc ggcttcaatg gaggcgttca atgagagcag gttggtgcga
     1141 tcagacactt cagtcatgat ccgcgtcacc tgcgaaacag ttgcgaatgc cttttcaata
     1201 tcgtccatgg cctggctcaa gttttgcgcg gatcgagtga ccgattcgcc acctgtgcgg
     1261 gcttcttcga tctgggcctg caattcttca ccggctcctc gaatgctgcg aagtagtagt
     1321 tccacttcgg atgcttgagc gctcacctgg tctatcaatt cgatctggct ttctgtagcg
     1381 gagttggaca tatcaatgga tgccgcgaat tcttccatga cagagctcag gtccgaatag
     1441 gccgccgcat gcgcctgcac tttggaattg agtctggtga gaaattctcg taaccccagg
     1501 acagattcgc ggagcctgga tgcagtcatt tcttcctgat gctgcaaccg tgaaagccgt
     1561 tcctgcgcat gctgcaattc agactcagct gacgcgcgac tttgcctcga tcgacgcaac
     1621 tgaatggcaa gccacagaat gacaagaagt gcaaacagga gcgcagccca ggcagtcgaa
     1681 gcataggaac tgaggagtgt cacccaggcg gagatattca caaaaccgag cagacgacga
     1741 cgggtggtgt cgtccattct ttgtcgaagg ctcgaaagct tgacagaacc aaatgagcgg
     1801 acgtcttcag gcgatatgtg gatttcgcgc agactgcagc tattgattct tactattttt
     1861 tactccactg ccactttcgc gggcacagtg actgtaacgg atgcaaacat ttc
//
LOCUS       DAOQJL010000677         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51116_length_1895_cov_3.883152, whole genome shotgun sequence.
ACCESSION   DAOQJL010000677 DAOQJL010000000
VERSION     DAOQJL010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51116_length_1895_cov_3.883152"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..388)
                     /locus_tag="PLG78_19575"
     CDS             complement(20..388)
                     /locus_tag="PLG78_19575"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNE25384.1"
                     /translation="MRRILYFCLSILTLLAGVWILKNPQYTLVRGTAGDILVVCLMFF
                     LFKILRDFESKKLAALLFLFACFVESLQYFHFADWLGLERGLLRTMLGSTFDFADIAA
                     YLAGAVFSVLVDRVVIAYSA"
     gene            473..>1895
                     /locus_tag="PLG78_19580"
     CDS             473..>1895
                     /locus_tag="PLG78_19580"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE25385.1"
                     /translation="MIGRLLIVLLLFTSYGSVFAEMLPPEKWSYIQTGRPSLWTWKEL
                     ESKTWTRATKLSFSGRQGNMVYLKTTIPSLEIADPSIYISLVSQSFEVYCNGNMIYQF
                     GNPNRGREGFSGWPAHIIPIPKSCDAKSTIIFKIYSDLMLLGFSGPSDPEYANRADIE
                     AKIVKRDIDRFSLGFLYIMTGAFSCFMYARRRKQISYLTFGAFALCAGIYSVTNRTLE
                     IQHLIWHAPMALYYIEYTTLFLLPATIALFFREIAVEGRVYAAIALVHVLYFSGAMIL
                     DLTLVPLYHALLPFFNVALISAIIGLIFMVREIKRGNKEARTLSFGLTLFVLTAIYDL
                     LGNLGYIPWPRQLVSWGFFAFMLSMIYVLEVRFSADQDRLTLYAAELEGAKANLEEKV
                     ELRTDELRKSLETVRELKTAQDGDYFLTSLLLRPLARNAADSGIIEVDTLTKQKKQFE
                     FKNRKYEIGGDISISDTIELSGKR"
BASE COUNT          519 a          490 c          428 g          458 t
ORIGIN      
        1 aaagccggga ttcccacgat caagcagaat aagcgattac aacgcgatcg acaagcactg
       61 agaacacggc ccctgccaga taggcggcta tgtccgcgaa atcaaatgta gatccaagca
      121 tagtcctgag cagtccacgc tcaagcccga gccagtctgc gaaatgaaag tactggaggc
      181 tctctacaaa gcaggcgaaa agaaacaaga gcgctgcgag tttctttgat tcaaaatcgc
      241 gcaagatctt gaacagaaag aacatgagac aaaccacgag gatatcgcct gcggtgcctc
      301 tgaccagagt gtattgcgga ttcttcaaga tccagactcc tgccagaaga gtcaaaatgg
      361 ataagcaaaa atatagaatt cgcctcatcg ggtcaccagc atcatgacgc gaagacgaac
      421 tgcaatgcga aaacaaaggt tgcggcgcaa tcgcccaggt ttacgatgag ccgtgatcgg
      481 gcgcctgctc atcgttctac tcttgttcac ttcttacggc tccgttttcg cggagatgct
      541 gcctccggaa aaatggtcct acatacaaac gggaaggcct tctctctgga catggaaaga
      601 acttgaatcc aaaacgtgga ccagggctac aaaactctcc ttctcaggcc gccaggggaa
      661 tatggtatat ctaaagacaa ccattccttc gctcgaaatc gcggacccgt ctatttacat
      721 ctctctagtt tcccaatcat ttgaagtgta ctgcaatgga aacatgatct atcaatttgg
      781 aaatcccaat cgcggcagag aagggttcag cggatggccg gctcacatca ttcccatccc
      841 aaaatcctgc gatgcgaaga gcactatcat tttcaaaatc tactctgacc tgatgctact
      901 cggattttcg ggcccgtcag atccagaata cgcgaatcgc gcggacatcg aagcgaaaat
      961 agtcaaacga gacatcgatc gcttctctct tggctttctt tatatcatga ccggagcttt
     1021 ttcctgtttc atgtacgcgc ggagaagaaa gcaaatctct tacctcactt ttggcgcatt
     1081 cgctctttgt gcgggaatct attcggtaac caatcgcact cttgaaatcc agcatttgat
     1141 ctggcatgca cccatggctc tctactatat agagtacacc actctctttc tgcttcccgc
     1201 aacaattgca ctcttctttc gcgagatcgc cgtggaaggt cgagtctacg cagcgatcgc
     1261 tctggtgcat gtgctctatt tctcaggggc tatgattctt gacctgactc ttgtgcctct
     1321 ttaccacgca cttcttccct ttttcaacgt agccttgatt tcagcaatca tcgggttgat
     1381 atttatggtg cgcgaaatca aacgcggaaa caaagaagca cgaacgctca gctttggcct
     1441 aactctcttt gtattaaccg cgatctatga ccttctggga aatcttggat acattccgtg
     1501 gcccagacag cttgtttcct ggggattttt cgcgttcatg ctatccatga tttatgttct
     1561 ggaagtgaga ttctccgccg atcaagatcg cctgacgttg tacgccgctg aactggaggg
     1621 ggcaaaagca aacctggaag aaaaagtaga gctccgaaca gacgaactac gaaaatctct
     1681 ggaaacagtg cgagaactga agactgctca ggacggggac tacttcctca cttctctcct
     1741 tctgcgtcct ctcgcgcgca acgcggcaga cagcggtatc atagaagtag acacgctgac
     1801 aaaacagaaa aagcaatttg aattcaagaa tcggaaatat gaaatcggcg gggacatttc
     1861 aatcagtgac acaattgaac tttccggcaa acgct
//
LOCUS       DAOQJL010000678         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51217_length_1893_cov_3.506529, whole genome shotgun sequence.
ACCESSION   DAOQJL010000678 DAOQJL010000000
VERSION     DAOQJL010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51217_length_1893_cov_3.506529"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLG78_19585"
     CDS             <1..253
                     /locus_tag="PLG78_19585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE25386.1"
                     /translation="LYYRGLCLERLGRENEATPFLEKAIELDQQHVHALKLLGNVYFK
                     LGKYRESALSLERAVELAPDQEKLKAALGKVKQRMDRLN"
     gene            complement(316..1359)
                     /gene="dctP"
                     /locus_tag="PLG78_19590"
     CDS             complement(316..1359)
                     /gene="dctP"
                     /locus_tag="PLG78_19590"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNE25387.1"
                     /translation="MRIHTMSSYRPYAALCVFALAFFHAALNPLTAEVELRVATLAPS
                     GSNWMSEMEKSSMEIKDKTNGRVTVKYFPGGQQGDERDYVRKIKLGQLDGATVTSVGL
                     SMIDESIRVLELPDMFESAEELDYVADKIWPHFQAKFKAKGFRLGARGEVGWTYFLSK
                     NKVESLSQLRAQKAWVWGDDVIARTLFANLGMNGVPLGVPEVDAALTTGKVDMCYGSP
                     LAAVALQWYTKIKYITSMPLNFSVGATVYWEDSINKVTAEDQKTIEEIDRASSKKIRK
                     VARKSNEDAINTMKRRGVAVVETPAQMVTEFGNHAEQIRTTLTGKAFSKQDLDLVLRY
                     RSDHRIKRGGKPK"
BASE COUNT          432 a          525 c          467 g          469 t
ORIGIN      
        1 actgtactat cgtggccttt gtctggaaag actcggacgc gaaaacgaag ccactccatt
       61 tcttgaaaaa gcaattgagc tcgaccagca acatgtgcat gcactcaagc tgctggggaa
      121 tgtttatttc aaacttggga aatatagaga atccgcgctc tctctggaga gagcagtcga
      181 actcgcgcca gaccaggaaa aactgaaagc ggctctcgga aaagtgaagc agaggatgga
      241 tcgcctgaat tgacgtctcg tgcgccttcc agcagaagga atttcgcgct gcgaaattcc
      301 ttcatgcaat ccgcgctact ttggttttcc gcccctcttt atgcgatgat cactccgata
      361 acgcaaaacc agatccagat cctgttttga gaacgccttt ccagtcaacg tcgtgcgtat
      421 ttgttctgca tgatttccaa actcggtgac catttgagca ggagtttcca ctaccgcgac
      481 tccgcgtcgt ttcatcgtgt tgatggcgtc ttcgttactt tttctcgcga ctttgcgaat
      541 ctttttagaa gaggcacgat ctatttcttc tattgtcttt tgatcttccg cagtgacttt
      601 gttgattgag tcttcccagt atacggtcgc gccgacggag aagttcaatg gcatggaagt
      661 tatatacttg attttggtat accattgcag agccacggca gcaagcggcg atccatagca
      721 catgtcaacc ttaccggtcg tcagagcggc atccacttcc ggtactccca gaggcacacc
      781 gttcattccc agattggcga ataacgtgcg agcaatgacg tcgtcacccc agacccaagc
      841 cttttgtgcg cgaagctgcg aaagtgattc cactttgttc ttagacagaa agtatgtcca
      901 gccgacctct cccctggcgc ccagcctgaa tcctttcgct ttgaactttg cctgaaagtg
      961 aggccagatt ttgtccgcaa cgtagtccag ttcttcagca ctctcgaaca tatccggcag
     1021 ttcgagcacg cggatcgatt catctatcat agagagtccg accgatgtga cagtcgctcc
     1081 atcaagctgg ccaagcttga tcttgcgaac gtagtcgcgc tcatctccct gttggccgcc
     1141 tgggaagtat ttgaccgtga ccctaccgtt agtcttgtct ttgatctcca tagaagattt
     1201 ttccatttcc gacatccagt tgctaccgct cggggccagg gtcgcgacgc gcaattccac
     1261 ctcagcagtc aaaggattca aggcagcatg aaagaaggcc agtgcgaaca cgcaaagggc
     1321 ggcgtatggt ctatatgagg acattgtgtg aatgcgcatg ccttcactcg cctttaccag
     1381 atatttacgt caaggagatt cagcgtctcg tgcggcctcg aacgcaggga ctgctcggac
     1441 cgcgctcctc cgggctccgc attcgcttcg tccgtccgtg atgaggaaat gtcgcataac
     1501 gggaattttc gcacgaacgg atgcttcgca cccttcagat cgctggccgg gagttgtctg
     1561 gacttgaaaa tgccgcgcaa gggcaggcaa atgatctgcc gggaagaaga tcaggtcttc
     1621 cgtagcccgg agtgccccgg atttatcacg cactcacatg acgcattaca tgcaaccgcg
     1681 ggtattatct cgcacttttt atgcgtgata aaacgcgccg gggagcgacc acggacgctc
     1741 gcgatcgcgc ctcgacaaaa aacgctgtat ccatcgcgaa tccttcccag aattcggcca
     1801 ccggaatatg cgtgcatttg catcgcccac gccgccggat ttatcccgca atcctgggac
     1861 gtattgactg caaccgcggg tactatctcg cac
//
LOCUS       DAOQJL010000679         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51339_length_1891_cov_3.379630, whole genome shotgun sequence.
ACCESSION   DAOQJL010000679 DAOQJL010000000
VERSION     DAOQJL010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51339_length_1891_cov_3.379630"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1891)
                     /locus_tag="PLG78_19595"
     CDS             complement(<1..>1891)
                     /locus_tag="PLG78_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNE25388.1"
                     /translation="LTQEIKKEESPLQKQLKGTVYAIALLAFTLGAAFLALGWVFGGL
                     SFLQAFVFFIGLFVANVPEGLLPTVTLSLAMGVSRMAKRNALVKNLSSVETLGCTTVI
                     CCDKTGTLTQNLMMVTQIYADGKTISVDGIGYEPMGSFHSESGPISSDELSRKPVLTG
                     LFSCAYNCNNARIEKHANAYRVLGDPTEGALVALANRAGVRGTHSRLHLNPFESVRKR
                     MSVVVRRDGDIRKTVYAKGAPMEMLERCKLIQEGNNTRLLTEGDRAAIKEKNDEFASS
                     GLRVLALAFRNDDGIRDISEYNAENCETDLVFLGLAAMSDPVRPQVPGAIEICHAAGI
                     RVIMITGDYALTAQSIGKRIGLGSGAAGGNLPVFAGVDIAQMTDESLAEILKAGETVF
                     ARVSPDQKLRIVTLLRNLGEIVAVTGDGVNDGPALKRADIGIAMGLRGTDVAKEASQI
                     ILTDDNFASIAAAIEEGRGIFENIKRFAAYVLSSNPQEMYPYILWMLFPGTPLAMTVM
                     GVLAVDVGTDLIPAMGLGIEPPEKGIMEKPPRKKGEKLLSVGFILQNYFVQGSILAFA
                     CFATYFYFGWALGLFAGGFSLSHLPSSPAGLDMKLASFVYLQSLTAYFFPTVATQIGN
                     VMCRRS"
BASE COUNT          457 a          494 c          493 g          447 t
ORIGIN      
        1 agaccttctg cacattacgt ttccaatctg tgtggccaca gtgggaaaga agtaagccgt
       61 caaagactga agatacacaa acgatgcgag cttcatgtcg aggccggcag gcgaagaggg
      121 aaggtgactc agtgagaatc cacctgcgaa aagaccaaga gcccatccaa aatagaagta
      181 ggtagcaaag caggcaaagg caagaatgct gccctgaacg aagtagttct gcagaatgaa
      241 tcctacggac agaagtttct ctcctttctt tctaggaggc ttctccatga ttcctttctc
      301 tggcggctca attccaagcc ccatggcagg aatgagatcg gtgcccacat ctaccgcgag
      361 cactcccatg acagtcatag ccagaggcgt gccaggaaaa agcatccaca ggatgtacgg
      421 atacatttcc tgtggattgc tcgaaagaac atacgctgca aatcgtttga tgttctcgaa
      481 aattccacgc ccttcctcaa tggctgctgc gatcgatgca aagttgtcgt ccgtgagaat
      541 gatctgtgaa gcttctttgg ccacgtcggt gccgcgaagg cccatcgcaa ttccaatgtc
      601 cgcccgtttc agtgccggtc cgtcgttgac tccatctcca gtgactgcta caatttcccc
      661 cagattgcga agaagagtga cgatgcgaag tttttgatcc ggagaaacac gtgcgaacac
      721 agtttcgccg gccttcaaga tttccgcgag gctttcgtct gtcatctggg caatgtccac
      781 accggcaaac acgggcaggt tccccccggc ggctcctgaa cccaggccaa tgcgttttcc
      841 aatgctctgt gcggtcagag cgtagtcccc tgtgatcata atgacacgga tgccagctgc
      901 gtgacagatt tcaatggcgc ctggaacctg tgggcgtact ggatctgaca tggctgccag
      961 accaagaaaa acaagatcgg tctcacaatt ctctgcgttg tattctgata tgtcccggat
     1021 accatcgtca ttgcgaaatg caagtgccag gacgcgaagg ccactggatg caaattcgtc
     1081 attcttctct ttgatggcgg cgcggtcccc ttccgtaagc aaacgtgtgt tgtttccttc
     1141 ctggatcagt ttgcagcgct cgagcatttc catgggtgcg cctttggcat agactgtttt
     1201 tctgatatct ccgtctcgcc gcacaaccac gctcatccgc tttcgcacag attcaaaagg
     1261 attcagatgc aatctgctgt gggttcctcg aacaccagcg cgattggcca gggcgaccag
     1321 ggctccttcg gttggatcgc ctaacactcg ataggcgttt gcatgctttt caatgcgcgc
     1381 attgttgcaa ttgtaggcac aggaaaagag cccggtaagc acaggcttgc gagagagttc
     1441 gtcggaggat atgggccctg attcagagtg aaagcttccc atgggttcgt agccaattcc
     1501 gtctactgag atggtctttc cgtcagcgta gatctgcgtg accatcatca ggttttgtgt
     1561 cagagtgccc gttttatcgc agcaaatcac agtggtgcat ccaagtgttt ccacagaaga
     1621 cagatttttc accagagcgt ttcgctttgc catgcgcgaa acacccatgg ccagagatag
     1681 agttacggtg gggagtagcc cctcgggcac gttggcaacg aacaggccaa tgaaaaagac
     1741 gaaagcctgg agaaaagaga ggcctccgaa tacccagccg agtgcgagaa aggcagcacc
     1801 cagagtaaaa gccagaagtg caattgcata cactgttcct ttgagctgtt tctggagagg
     1861 gctttcctct tttttgattt cctgggtcag g
//
LOCUS       DAOQJL010000680         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51487_length_1888_cov_4.120022, whole genome shotgun sequence.
ACCESSION   DAOQJL010000680 DAOQJL010000000
VERSION     DAOQJL010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51487_length_1888_cov_4.120022"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1146)
                     /gene="leuB"
                     /locus_tag="PLG78_19600"
     CDS             complement(52..1146)
                     /gene="leuB"
                     /locus_tag="PLG78_19600"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNE25389.1"
                     /translation="MSHRIAVLAGDGIGPEVMPSAIEILSRALGSEFSNFTFEEAPVG
                     GAAIDQTGEALPASTLKLCESSDAILFGSVGGPKWEHLPPEKQPERASLLPLRKHFKL
                     FANLRPVALYPELEASSPLKAERTVGTDLLVIRELTGDVYFGQPKGREGSGPDEKGFD
                     TMVYHRYEIERIAKVAFESAVGRKRKVTSIDKANVLTSMVLWREVVTQFGKKFNEESK
                     VQVELDHMYVDNAAMQLILKPSRFDVVLCGNMFGDILSDEASVLGGSLGMLASASLSE
                     KQGFGLYEPAGGTAPDIAGKGIANPIAQILSGALLLRYSFGKGDAASKIEAAVKSAIS
                     AGARTIDLAGPGETSISTKEMTQEIARRIS"
     gene            complement(1176..1637)
                     /locus_tag="PLG78_19605"
     CDS             complement(1176..1637)
                     /locus_tag="PLG78_19605"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE25390.1"
                     /translation="MPALLRSDSLPDILYSEVIRAPVAEVFKALTTARLMDDWGAGPA
                     RFQAKPGGKWSLWDGVMQGVVKEVHAPDRVVFTLREIHWNERDPDSLVIIELSEIHRG
                     TRIELRHTNLPSRKIRQLHEDSWGDVYLGPVKAYLEATFSPFVKTRTGKLT"
     gene            1704..>1888
                     /locus_tag="PLG78_19610"
     CDS             1704..>1888
                     /locus_tag="PLG78_19610"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNE25391.1"
                     /translation="MTAKVLLVFLGGGLGSVLRLAAGIHWNRQYPYGTLAVNLIGCLV
                     IGAAAETFTRLSMLDSSA"
BASE COUNT          449 a          499 c          465 g          475 t
ORIGIN      
        1 cgcaaatagc gatgcgatat gccttgccgt taggcgccac tccgtccggt ttcatgagat
       61 cctgcgcgca atttcctggg tcatttcttt tgtgctgatg gatgtttctc ctggacccgc
      121 cagatcaata gttctggctc ctgcactgat ggcagacttc acggccgcct caatttttga
      181 ggcggcgtcc ccttttccga atgaataccg gagtaagaga gcgccggata gaatttgtgc
      241 gattggattt gcgattcctt tgccagcgat gtcaggagcg gtgccgccgg ccggctcata
      301 caaaccgaat ccctgctttt cagaaaggga agcgctggcc agcattccaa gagaaccgcc
      361 cagcacactg gcctcatcgc tcaaaatatc tccgaacata ttgccgcaga gcactacgtc
      421 aaaacgcgaa ggtttgagaa tgagctgcat ggcggcgttg tccacgtaca tgtgatcgag
      481 ctccacctgc accttgcttt cttcgttgaa tttctttcca aactgagtga ccacttcacg
      541 ccacagcacc atagaggtca gaacgtttgc cttatcaatg cttgtaactt tgcgcttgcg
      601 accgactgcg gattcaaaag caacctttgc gattctttca atttcatagc gatgatacac
      661 cattgtatca aagcccttct catccgggcc gctgccttct cttccctttg gctggccaaa
      721 gtaaacatcc ccggtaagct cgcgaatcac aagcaaatcc gttccaacag tgcgttctgc
      781 tttgagagga gaagatgctt ccagctctgg atacagtgca actggtctta gattcgcaaa
      841 tagtttaaaa tgtttacgaa gtggcaggag agaagctctt tctggctgtt tttctggagg
      901 aaggtgttcc cattttggac cgcctacaga cccgaacagg attgcatcag aagactcaca
      961 caacttgagg gtggaagccg gaagggcttc tcccgtttga tcaatggccg ctccgcctac
     1021 gggagcttct tcaaatgtaa aattggaaaa ttcagaaccc agagctctgg ataaaatttc
     1081 gatcgcagaa ggcatgacct ccgggccaat tccgtctcct gcgagaacag cgatacgatg
     1141 agacatggat cattcgggcg aaaacgcccg cactgtcaag tgagctttcc agtgcgtgtc
     1201 tttacgaatg gactgaacgt tgcctcaagg taagccttta caggtcccag ataaacgtct
     1261 ccccaggaat cttcatgaag ctgtctgatt tttctagaag gtagatttgt gtgacgaagc
     1321 tcgatgcgag tgcctcgatg aatttcagaa agttcaatga tcacgagaga atccggatcg
     1381 cgttcattcc agtgaatttc gcgcagagta aaaacaacac gatcaggagc gtgcacttct
     1441 ttgactacgc cctgcatgac tccgtcccag agagaccatt taccgcctgg ctttgcctgg
     1501 aatcgcgcag gtcccgctcc ccagtcgtcc atgagacgcg cagtcgtgag cgccttgaat
     1561 acttcagcaa caggagcacg gatgacttcc gaatacagga tatcaggcag gctgtcgctt
     1621 ctcaaaagtg ctggcatggt ttactccctt aagtacgatc ggcaggaatt caaaagttcg
     1681 cttatctttt tttggacgat tccatgacag caaaagttct acttgtattt ctgggcggcg
     1741 ggcttggcag cgtgcttcgc ctggctgcgg gcattcactg gaatcgccag tatccatacg
     1801 gaactctcgc agtgaatcta attggatgcc tcgtcatcgg agcggctgca gaaacattca
     1861 cgcggctctc catgctggac tcaagcgc
//
LOCUS       DAOQJL010000681         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51532_length_1887_cov_4.400655, whole genome shotgun sequence.
ACCESSION   DAOQJL010000681 DAOQJL010000000
VERSION     DAOQJL010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51532_length_1887_cov_4.400655"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PLG78_19615"
     CDS             complement(<1..1509)
                     /locus_tag="PLG78_19615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE25392.1"
                     /translation="MSGTIVILGGAMAGPTAAARAREINENARIILIEQGTRVSYAAC
                     GLSYYLSGEVQSLEDLNHERSDFFRQVYNIEVWTETEAIRLDSIKKILTVKQSKGEMD
                     LSYDSLIFAAGGSSIAPLEIQGVENQTVFRTLDDLVKIRSALDSGKKKFVILGGGPIG
                     LEAADGLIRAGAEVTIVEKGPQILGTFGDKISRVVRGILETSANVITSARDESFEIHE
                     GRITSVTVDGRRIEADYVISAMGIVPRTDILKEAGAGIHSDGTVVINESCATTLKDVY
                     ACGVCVSVPHSGSFSWTPQASVADKTAQVAGENAAGGDAKLASFVGSLLLRLPNMSAG
                     RVGPSFRNLGLAAGNRLGRAFVHAYDVEPYMPHAKPLMLELFYEKESGKVLALEAAGS
                     GVERRLDAAAVAIAGSLTIQQLAKLDLAYSPALGTARDALNVAATVAVQAERGRTATV
                     EPADLQARTQDYCIVDTGKKARGSVHLHIPLEELRGKIEEIRSALLKSGARQI"
     gene            complement(1546..1827)
                     /gene="hisE"
                     /locus_tag="PLG78_19620"
     CDS             complement(1546..1827)
                     /gene="hisE"
                     /locus_tag="PLG78_19620"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNE25393.1"
                     /translation="MKFLEDLEKLLYQRKHELPDGSYTTTLFKGGEDRILKKVAEESG
                     EVIIAVKNSDAKEITHEAADLIFHLLVALVQKGVPLSAIEAELISRHKK"
BASE COUNT          430 a          551 c          435 g          471 t
ORIGIN      
        1 gatctgtctt gctcccgatt taagaagtgc ggacctgatt tcttcgattt tgccgcgcag
       61 ctcttctaaa gggatgtgca gatgaacact cccgcgcgct ttttttccag tgtccacgat
      121 gcagtagtcc tgggtgcggg cctggagatc tgccggttcc acagtggccg tgcgccctcg
      181 ttccgcctga actgccacgg tggccgctac attgagagcg tctctggctg ttcccagcgc
      241 cggactgtat gccagatcaa gtttcgcaag ctgctggatg gtcagagaac ctgcaatcgc
      301 cactgcggcc gcgtccagac gtctctctac gcctgatcca gctgcctcga gtgcgaggac
      361 cttgcccgat tccttttcat aaaacagctc gagcatcaaa ggctttgcat gaggcatgta
      421 tggctccaca tcataggcat gaacaaacgc gcgacccagc ctgttgccag cagccaaccc
      481 cagatttcta aaagacggac caactcttcc cgcggacatg ttgggaaggc gaagcaagag
      541 agacccaaca aacgatgcga gctttgcatc cccaccggcc gcgttttctc ccgccacctg
      601 cgcggtctta tccgccacgg aagcctgagg agtccaggaa aagcttccgg aatgcggaac
      661 actcacacac actccgcagg catacacgtc ctttagagtt gtggcgcatg attcattgat
      721 caccactgtg ccatccgaat gaatgccggc gcccgcctct ttcaagatat ctgtgcgggg
      781 aacgatgccc atggcggaaa tcacatagtc tgcttcaatt ctgcggccgt ccacagttac
      841 agacgttatg cggccttcat gaatctcaaa cgattcatct ctcgcagatg taatgacatt
      901 cgcggaggtt tctagaattc cacgcacgac tctagaaatc ttatctccga acgttcccag
      961 aatctgtgga cctttttcta caatagtaac ttctgcgccg gcgcggatga gcccgtctgc
     1021 ggcctcaagg ccaattggcc ctcctccaag aattacaaat ttcttttttc ccgaatcgag
     1081 agcggagcga atcttcacca gatcatcgag cgtgcgaaat acagtctggt tctccactcc
     1141 ctggatttcc agaggtgcaa tgctcgatcc acccgcggca aagatcagtg aatcatagga
     1201 aagatccatt tccccctttg attgtttgac tgtgaggatt ttcttaatgc tgtcgagacg
     1261 aatggcttct gtctccgtcc acacctcaat gttgtatacc tgtctgaaaa aatcagaacg
     1321 ctcgtgattc aaatcctcaa gagattgaac ctctcccgaa aggtaataag aaagaccgca
     1381 ggccgcatag ctgacgcgtg ttccctgctc aatcaagatg atccgcgcat tttcattgat
     1441 ttcgcgtgca cgggcagccg cagttggacc tgccatggca ccacccagaa tgacaatcgt
     1501 accggacata gactcccccg aactataaac tttcttaaag attgactatt tcttgtgacg
     1561 cgagatgagc tcagcctcga tcgcgctcag cggaactccc ttttgcacga gagcaacgag
     1621 caaatgaaag atcaagtccg ccgcttcatg agtgatttcc ttcgcatcgc tattctttac
     1681 tgcgatgatc acttccccgg actcttctgc cactttcttc aggatgcgat cttccccgcc
     1741 cttgaacaga gtcgtagtgt aggaaccgtc cggcaattcg tgcttacgct ggtagagtaa
     1801 tttctcaaga tcttccagga atttcatact ctgacctgca ctccacgttc tctcatgatc
     1861 ttcttcacgc tgtccactgt tgtttct
//
LOCUS       DAOQJL010000682         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_51538_length_1887_cov_3.967249, whole genome shotgun sequence.
ACCESSION   DAOQJL010000682 DAOQJL010000000
VERSION     DAOQJL010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51538_length_1887_cov_3.967249"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1501
                     /locus_tag="PLG78_19625"
     CDS             2..1501
                     /locus_tag="PLG78_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE25394.1"
                     /translation="MFFFGIHSYIMVYLYRKNEKYCVSGQDVHMDPIDISKTPESEIP
                     VVTVQLPVFNEFYVVDRLIEATTQMVWPKSKLEIQVLDDSTDESKDKAATLVEMYRSR
                     GFDIHHIHRTNRQGHKAGALKEGLEVARGEFVAIFDADFLPASDFLVKTVPYFQEPGI
                     GMVQTRWGHINDDYSVLTKAQSFGIDGHFMIEQVARNCNNLWMNFNGTGGIWRRQCIV
                     DAGNWQSDTLTEDFDLSYRAELAGWSFRYFKDIVNPAELPATMSAFKSQQFRWCKGSI
                     QTAVKLIPTILRSKFSRKIKSEAIVHLLNYSVHPLMMINILLCLPLLLMDVWTGFSIY
                     DLSLAVLFGAATLMSLGTFGPVVFYIYSQRELYPDWKKRIWWMPVLMMIGTGIAVVCG
                     RAFLEAILGIQSTFKRTPKYRIENKSDKLIERMKYKLPLDKTVFIEFFMGFYCVACIY
                     FSIVKNKPFVVPFMLIYAAGFFYVALSSLSESFVRVKRPAIDSAADARA"
     gene            1536..>1887
                     /locus_tag="PLG78_19630"
     CDS             1536..>1887
                     /locus_tag="PLG78_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25395.1"
                     /translation="MSLLSGGECFVSPFPCSFPSSRVNLLEQAELDLCRGRKVDSLNW
                     LVPNGAESFFDFVFEQAQKGLRVRGDSAAPIEAIHSGGKSWAERFPILHVSEHSTIPS
                     RESARPVSRFIYLNP"
BASE COUNT          444 a          472 c          431 g          540 t
ORIGIN      
        1 gttgttcttt ttcgggatac actcttacat catggtgtat ctctatcgga agaatgaaaa
       61 atactgcgtg tccggccagg atgtgcacat ggatccaatt gatatttcaa aaactcctga
      121 gtcagaaatt ccagtcgtta ccgtacaact tcctgttttc aatgaattct atgtcgtaga
      181 ccgcctcatc gaagctacga cacaaatggt atggccaaaa tccaaactgg aaatccaggt
      241 actggacgac tcaacagacg aaagcaagga caaagcagcc actctggtgg aaatgtatcg
      301 ttcgcgtggt tttgacattc atcacatcca tcgcaccaat cgtcagggcc acaaagcagg
      361 cgcactcaaa gaaggactcg aagtggctcg cggagaattc gttgctatct ttgacgcaga
      421 cttccttcca gcatctgact tccttgtgaa aacagttcct tacttccagg agccagggat
      481 cggcatggtc cagactcgct ggggacacat caatgacgac tattccgttc tgaccaaagc
      541 acaatcattt gggatcgacg gtcacttcat gatcgaacag gttgcgcgca attgcaacaa
      601 tctctggatg aacttcaacg gaaccggtgg catctggcgt cgtcagtgca tcgtagatgc
      661 gggcaactgg cagtcagata ctctcacaga agactttgat ctttcctatc gcgcagaact
      721 cgcaggctgg agcttccgtt atttcaaaga tattgtaaat cctgcagaac ttccggcaac
      781 catgtctgct ttcaagtcgc aacagtttcg ctggtgcaag ggttccatcc agactgcagt
      841 caaactcatt ccaacgatcc ttcgctctaa attttcgcgc aagatcaagt cggaagcaat
      901 cgttcatttg ctcaactact ctgttcatcc tctcatgatg atcaacatct tgctctgtct
      961 tcctcttctg ctcatggatg tgtggacagg atttagcatt tatgatcttt ctctggctgt
     1021 tctttttggt gcggccactc tgatgagcct gggaacgttt ggtccggttg ttttctacat
     1081 ctattctcag cgtgaacttt atccagattg gaaaaagaga atctggtgga tgcctgtttt
     1141 gatgatgatt ggaactggaa tcgcagttgt ttgcggtcgt gcgttcctgg aagcgattct
     1201 tggaattcag tctactttca aacgtactcc aaagtaccgt attgagaaca aatcagataa
     1261 gctgattgag cgcatgaaat acaagctgcc tctagataag actgtattca ttgagttctt
     1321 catgggcttc tactgcgtcg cctgtatcta cttctccatc gtgaagaaca agccattcgt
     1381 agttccattc atgttgatct atgccgccgg cttcttctat gtggctctct cctctctctc
     1441 cgagtctttc gttcgcgtga agcgtcctgc gatcgactcg gcagctgatg ctagagcctg
     1501 atcgctggcc tactttagaa ttttccctta cgggaatgtc tctcctctcg ggaggagagt
     1561 gctttgtttc tccttttcca tgtagctttc catcttctcg cgtaaatttg ctcgagcaag
     1621 cagagctgga tctatgcaga ggccgcaaag tggactctct gaactggctc gtaccaaacg
     1681 gagcggaatc cttctttgac tttgtatttg agcaggcaca gaagggtctt cgcgttcgag
     1741 gcgattctgc agcgcctatc gaagcaattc actccggcgg taaatcctgg gcggagaggt
     1801 ttcctattct ccatgtatct gagcattcca caattccatc acgcgaaagt gcgcggcccg
     1861 tcagtcgttt catctatttg aacccgg
//
LOCUS       DAOQJL010000683         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_52438_length_1870_cov_3.780165, whole genome shotgun sequence.
ACCESSION   DAOQJL010000683 DAOQJL010000000
VERSION     DAOQJL010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52438_length_1870_cov_3.780165"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..674
                     /locus_tag="PLG78_19635"
     CDS             21..674
                     /locus_tag="PLG78_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE25396.1"
                     /translation="MERAVRLTTVKRRPLRLPRRVRGSISRREILEAAVAVLSEKGVE
                     GLSMRHIAQHMGCSVASPYAYFSSQEEIIRHLITEGEKKLTLDLKAAQECSHDVYVQL
                     DAIAHAYWNFSSENRELHKLMFNMGGGRLYRQVFPSLPTSYRVFLETIREGIISGAIP
                     YPRTSYPSIARTMWSWMYGLIVLEMNEVLRRRKTSDPVKEGIALFHHLLRSGANFSG"
     gene            706..>1870
                     /locus_tag="PLG78_19640"
     CDS             706..>1870
                     /locus_tag="PLG78_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNE25397.1"
                     /translation="MADAFDFDVIVIGSGFGGSVTTCRLAEKGYHVALLERGRKYGMH
                     EFPRRMDEMKSRLFWDPDDGYYGFMEFRTYPASDAFSVCASGLGGGSLIYANVLMEMP
                     AAFFAGWPGGITRETLNPFYARGLEMLEAAPYPYETNPYYRDTPKTEAFRAASDAVKD
                     DPDAIEPHRFLFPNLAIRFKGDFPGHQTLNQQGVVQSSCIKCGECDVGCNVHAKNTLD
                     LNYIARARNQALLGPDGKAAEVRTGALVKEISPLESGGYAVTYVNPLNQSEKTTIRAQ
                     NVVVSAGCVGTNALLLKMRKYKKLNHLSPMLGKKWCGNGDLEGTVLFSDREHPLNNGP
                     VITAAVQHRFKDYPDGFGHGLVLQEAGIPNFIAWFIAGRLPSIGFFYKLFVVPF"
BASE COUNT          443 a          462 c          502 g          463 t
ORIGIN      
        1 agtcttttcg tcttgatcgg atggaacggg cggtgagact gacgactgtg aaacgcaggc
       61 ctctccgcct tccccgcaga gttcggggat cgatcagccg ccgcgaaata ctcgaggcgg
      121 ctgttgctgt tttatcggag aagggagtgg aaggcctcag catgcgtcac atcgcccagc
      181 acatgggctg cagcgttgcc agtccttacg catatttttc aagccaggaa gaaatcattc
      241 ggcacttgat tacggaaggg gagaaaaagc tcactctcga tctgaaggcg gcgcaggaat
      301 gctcgcacga cgtatatgtt caactcgatg cgatcgctca cgcgtactgg aatttttctt
      361 ccgaaaaccg cgaacttcac aagctcatgt tcaatatggg cggtggccgc ctttacagac
      421 aggtttttcc ctcattgccc acaagctacc gcgtctttct tgagaccatc cgagaaggga
      481 tcatcagcgg tgccattccg tatccacgaa ccagctatcc gtcgattgct cgaaccatgt
      541 ggtcgtggat gtacggcctg attgttcttg agatgaatga agtgttgcga aggcgcaaga
      601 ccagcgatcc agtcaaagaa ggaatcgctc tttttcacca ccttcttcgc agcggcgcta
      661 actttagcgg ttgactgatc tgcaatcctg gtctgcaatc agttcgtggc tgatgcattt
      721 gacttcgatg taatcgtaat tggatctgga tttggcggct ctgtgactac gtgccgactt
      781 gctgaaaagg gataccacgt tgctttgctt gagcgcgggc ggaaatatgg aatgcacgaa
      841 ttcccgcggc gcatggacga aatgaagtcg agactgttct gggatccaga tgacggatac
      901 tatggtttta tggagtttcg tacctatccg gccagtgatg cgttttcagt ctgtgccagc
      961 ggcctcggtg gcggatcctt gatctatgca aacgtgctga tggaaatgcc cgctgctttt
     1021 tttgctggat ggcctggagg aatcactcgc gaaacactca atccattcta cgccagaggg
     1081 cttgagatgc tggaggcagc tccctatcca tacgaaacaa acccgtacta cagggatact
     1141 cccaagacag aagcttttcg cgctgcgtcg gacgcagtca aagacgatcc ggatgcaatc
     1201 gaaccgcatc gctttctttt tccaaatctc gccattcgtt ttaaagggga ctttccggga
     1261 caccagactc tcaatcagca gggagtggtg cagtcgagtt gcatcaaatg cggggagtgc
     1321 gatgtgggct gcaacgttca tgcgaaaaat actctggact taaactacat tgcccgcgcg
     1381 agaaatcagg cgctgcttgg accggacgga aaagcggcag aggttcgcac aggtgctctc
     1441 gtgaaagaaa tttctccgct cgaaagtggt gggtatgcag tcacctatgt aaaccctctc
     1501 aatcagtctg agaagacaac gattcgcgcg caaaacgttg ttgtttctgc aggctgcgtt
     1561 gggaccaacg cgctacttct gaaaatgaga aagtataaga aactaaacca tcttagcccg
     1621 atgctcggaa agaaatggtg cggcaatggc gatctagaag ggacggttct attttcagac
     1681 agagagcacc ctctgaacaa tggtcctgtg atcactgcag ccgttcagca cagattcaaa
     1741 gattacccgg atggattcgg tcacgggctt gttttgcagg aagcgggaat tccaaacttc
     1801 atcgcctggt tcatcgcagg tagattgcct tctatcgggt tcttctataa attgttcgtg
     1861 gttcctttct
//
LOCUS       DAOQJL010000684         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_53267_length_1855_cov_3.080000, whole genome shotgun sequence.
ACCESSION   DAOQJL010000684 DAOQJL010000000
VERSION     DAOQJL010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53267_length_1855_cov_3.080000"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLG78_19645"
     CDS             complement(<1..362)
                     /locus_tag="PLG78_19645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE25398.1"
                     /translation="MSPFWSRLSPFSAEDAVEVRLKSAPMFRDISDRGLKEIRSLCHL
                     RDYAAGEHIFREGEPGVGMYVILDGFIDIYHRDGKREKHLATLESGDFFGELALLEEL
                     PRTASAAAKNHARIIGFF"
     gene            462..533
                     /locus_tag="PLG78_19650"
     tRNA            462..533
                     /locus_tag="PLG78_19650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:494..496,aa:Pro,seq:tgg)
     gene            540..616
                     /locus_tag="PLG78_19655"
     tRNA            540..616
                     /locus_tag="PLG78_19655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:574..576,aa:Arg,seq:tct)
     gene            623..695
                     /locus_tag="PLG78_19660"
     tRNA            623..695
                     /locus_tag="PLG78_19660"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:His,seq:gtg)
     gene            819..900
                     /locus_tag="PLG78_19665"
     tRNA            819..900
                     /locus_tag="PLG78_19665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:853..855,aa:Leu,seq:tag)
     gene            909..980
                     /locus_tag="PLG78_19670"
     tRNA            909..980
                     /locus_tag="PLG78_19670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:941..943,aa:Gly,seq:gcc)
     gene            1050..>1855
                     /gene="tig"
                     /locus_tag="PLG78_19675"
     CDS             1050..>1855
                     /gene="tig"
                     /locus_tag="PLG78_19675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNE25399.1"
                     /translation="MQIVLKNTPEEVQQAYATAYEKAAKKVRVPGFRPGKVPMEVLER
                     ELGDAVAGDAAQILVASSMEKLLGEIKPPPISVPSFEVEQFDRKQGATYRGTFDTYPE
                     VKLGKYKKIKASQDTVEVLEEDIMAEIEKLRKDHSIMHTRDDGALAGDVLTIELDIRE
                     SGGKKKLYHNKDYKMVLSEPSPFPGLVEQLLGAKSGESKSYDQQMEESFPDPKFAGKL
                     VSVTANISTVQYPVLPELNDDLAKEVSEFDTLGALKNNIRETLTKQADNIL"
BASE COUNT          482 a          446 c          493 g          434 t
ORIGIN      
        1 ctgaaaaatc caatgattcg cgcgtggttc tttgccgcag cagatgcagt gcgaggtagt
       61 tcttccagta gtgcgagttc gccaaaaaaa tctcccgatt cgagagtggc cagatgtttc
      121 tcgcgtttgc catcgcggtg atagatgtca atgaatccat ccagaatcac gtacattcca
      181 acgccaggtt ctccttcgcg aaagatgtgt tcgcccgccg cgtaatcgcg cagatggcag
      241 aggcttcgaa tttctttgag acctctgtcc gagatatctc tgaacatggg tgcgctcttc
      301 aggcgcactt ccactgcgtc ttccgctgaa aaggggctca atctggacca gaaaggggac
      361 atctagaata aagatcggtc ctcgtagatt catgtcggat gcggaatgtt gtttttttgt
      421 ggactccaga caaaattcta aaaactcgtc cataaacctc acgggatgta gcgcaggggt
      481 agcgcatctg ctttgggagc agagggtcgt tggttcaaat ccaatcatcc cgattgtagg
      541 tgccggtagc tcagttggat agagcaactg ccttctaagc agtgggtcgg gggttcaact
      601 ccctcccggc gcaccagata cggcggatgt agctcagttg gtagagcatc agattgtggc
      661 tctgagggtc gcgggttcga gccccgtcat tcgcccatcc ctttcatcgc ttcgcgcgct
      721 ttcctttttt ctttttgcga agatagacca ttccgaatcg ctatcgcttg acactctgac
      781 tgcccgccag ttttatgtcc gccagaccgg aatctgaggc gagcgtggtg gaattggtag
      841 acacgccaga cttaggatct ggtgccgcaa ggtgtggggg ttcaagtccc ttcgctcgca
      901 gaaaaaacgc gggaatagct cagcggtaga gcacctcctt gccaaggagg gggtcgcggg
      961 ttcaaatccc gtttcccgct tatttttaca aacaagattg aagaggcgcg accctggaat
     1021 acaagacaaa acgccttgaa aacgcatccg tgcagattgt tctgaagaac acgccggagg
     1081 aagtgcagca ggcttacgcc actgcatatg aaaaagctgc aaagaaagtc cgcgtgcccg
     1141 gattcagacc gggcaaagtt ccaatggaag ttctggaacg tgagctgggc gatgctgttg
     1201 caggagacgc ggcgcagatt ctggttgcga gtagcatgga aaagctactc ggggaaatca
     1261 aacctcctcc gatcagtgtt ccttcttttg aagtagagca gttcgatcgt aagcagggag
     1321 ccacataccg aggtacgttt gatacgtatc cggaagtgaa gctgggcaag tacaagaaga
     1381 tcaaagcatc tcaagatacc gtggaagtcc tcgaagagga catcatggcg gaaatagaga
     1441 agcttcgcaa agatcattcg atcatgcaca caagagacga tggcgctcta gccggggatg
     1501 ttttgaccat cgagctggac atccgcgaat ctggcggaaa aaagaaactc tatcataaca
     1561 aagactacaa gatggttctt tcagaacctt ctccttttcc tggccttgtg gaacaactgc
     1621 tgggtgcaaa atctggagaa tcaaaatcat acgaccagca gatggaagaa agttttcctg
     1681 atccaaagtt tgcaggaaag ctggtgtctg ttaccgcgaa catttctact gttcaatatc
     1741 cagtgttgcc tgaactcaat gacgatctgg caaaagaagt gagtgagttt gatactctcg
     1801 gtgcgctaaa aaacaatatc cgcgaaaccc tgacgaagca agcagacaac atact
//
LOCUS       DAOQJL010000685         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_53848_length_1845_cov_5.239665, whole genome shotgun sequence.
ACCESSION   DAOQJL010000685 DAOQJL010000000
VERSION     DAOQJL010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53848_length_1845_cov_5.239665"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLG78_19680"
     CDS             complement(<1..830)
                     /locus_tag="PLG78_19680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE25400.1"
                     /translation="MLSSLKSIFETKPNELNALNAFRTFSVLGILVHHYWEAVRPSFL
                     GTSVMVDFLDRVPLNLLSVMDLFLMLSGFLISGGLWLDWKKNGTIHFGKYFTRRSFRI
                     FPLFYAALLVSFALTNAQIQLMSKQTLDAAGTSMLAELKQMKSHWWTDALFISNYTSH
                     KIMIHGWSLAVEQQFYTFLPLIAYFFLFRLSNKTRILSLCVLYFVPLVFRIIAAMNID
                     SEFGQMSKIYWPTHTRADSLILGILIMDLYYTSEWFRGKIADPMLRRIGFVLSLVLLG
                     "
     gene            930..1739
                     /gene="thiD"
                     /locus_tag="PLG78_19685"
     CDS             930..1739
                     /gene="thiD"
                     /locus_tag="PLG78_19685"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNE25401.1"
                     /translation="MSLAVVLSIAGSDSGGGAGIQADLKTFQALGCFGTTAITAITCQ
                     NTTGVRGVQAMTPEIVRNQILAVTEDFPVRAAKTGMLFSTEIIDAVSKALDDTSYKFP
                     IVVDPVMVASTGARLLREDAEKALASFLVRAAVITPNVPESEVLLGRKIVTLDDMEKA
                     ALDLHEMTGSAVLVKGGHRAEHGNVVFDVLYDGAHFEILEAMFQSPRNTHGTGCTLSA
                     GIAAGLARGLSIVESVREAKKFLVGALAGAPGLGHGTGPLNHLWMSGLTQN"
BASE COUNT          463 a          426 c          506 g          450 t
ORIGIN      
        1 attccgagaa gcacaagaga gagtacgaat ccaattcttc gcagcattgg gtccgcgatt
       61 tttcccctga accattcaga cgtgtaatac aagtccatga tcaaaatccc gagaatgaga
      121 gaatccgcgc gcgtatgcgt tggccagtag atcttgctca tttgtccgaa ttcagaatcg
      181 atgttcattg ctgctatgat gcgaaaaaca agcggcacga aatagagaac gcacaggctc
      241 agaatgcgcg ttttgttact gaggcgaaac aggaagaaat acgcaattaa tggaagaaac
      301 gtgtagaatt gttgctccac ggccagcgac cagccgtgga tcatgatttt gtgcgaagtg
      361 taattggaaa tgaagagagc atccgtccac cagtgtgatt tcatttgttt aagctctgcg
      421 agcatgctgg ttccggctgc atccagagtt tgtttggaca tgagctggat ttgcgcgttg
      481 gtgagagcga aggaaaccag aagagctgca tagaaaagtg ggaagatgcg aaaacttctt
      541 cgcgtgaaat atttgccaaa gtgaatggtc ccattctttt tccagtcgag ccagaggcca
      601 cccgagatca gaaagccact caacattaaa aacaaatcca ttacggaaag caggttcaac
      661 ggtacgcgat ccaggaaatc taccatcaca gatgtgccca gaaaggatgg cctcactgct
      721 tcccagtagt gatgaaccag aattccgagt acagaaaatg tgcgaaacgc attgagcgcg
      781 ttcaattcgt ttggcttggt ctcgaagata gatttgagcg atgagagcat tgcccttgta
      841 tcgaggatgc aggcggggcg aaaaggcaaa aatcacaaga tctcgtaaat gtagattgct
      901 ttatgtggtc ctgtgccttc cttgccttca tgagtctcgc cgtagtactc agcattgcag
      961 gatcggattc tggcggagga gccgggattc aggcggacct gaaaacattt caggctcttg
     1021 gctgctttgg caccactgct ataactgcca ttacgtgtca gaatacaacc ggtgtgcgcg
     1081 gtgttcaggc aatgacgcct gagatcgttc gaaatcagat tctggctgta acagaggatt
     1141 ttcctgtgag agcggcaaag accgggatgc ttttttccac tgaaatcatc gatgcggttt
     1201 caaaggccct ggatgatact tcctataaat ttccgattgt tgtggatcct gtgatggttg
     1261 cgtcaacggg cgctcgtctt ctgagagaag atgcggagaa ggctctcgct tcatttctag
     1321 ttcgtgcggc tgtgattacg ccaaacgttc ctgagagtga agttcttctt ggtagaaaaa
     1381 tagtaacgtt agacgacatg gagaaggcgg cactggacct gcacgaaatg acaggttcag
     1441 cggtgcttgt gaaaggcgga catcgggcag aacatggcaa tgtggtcttt gacgttctct
     1501 atgacggcgc tcacttcgaa attctggaag ccatgttcca gagtccgcgc aacacacacg
     1561 gaactggatg tactctttcc gccggcatcg ctgctggact ggcccggggc ttgtctatag
     1621 tagagtctgt gcgtgaagca aaaaagtttc tggtgggtgc gcttgcaggt gcgcctggcc
     1681 tggggcacgg aaccggcccg ctcaatcatc tctggatgag cgggctgact cagaattagc
     1741 ggcgtctgcc gcctttggaa aattcgcggt cgctattgta gtcgcgaccc ccgccattgc
     1801 cgcggtttcc gccgccgtcg cggcctccac cataccgttg attgc
//
LOCUS       DAOQJL010000686         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_54527_length_1834_cov_4.694210, whole genome shotgun sequence.
ACCESSION   DAOQJL010000686 DAOQJL010000000
VERSION     DAOQJL010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54527_length_1834_cov_4.694210"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLG78_19690"
     CDS             complement(<1..480)
                     /locus_tag="PLG78_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25402.1"
                     /translation="MHSKINPALILLAGVGLLYCNKPVPVQELAQARDEIAMAEEEHA
                     PEYSASNFQGARDALLASHQKLGSEEYDNAKQKAIDSFNQARAARVEASPKYTEATRA
                     TAEKTIAQADQAYAEELAKDDFSAAKKLYGEGTTAQGQAKAATGDVQLDTYRQAYRKF
                     "
     gene            complement(523..858)
                     /locus_tag="PLG78_19695"
     CDS             complement(523..858)
                     /locus_tag="PLG78_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE25403.1"
                     /translation="MEITRRASGDIVILDVNGEIDLYNAPEIKELINKLIEEQKYNVI
                     INLDRVSYLDSSGIGALISSLSNLKKYQGGLKIINVSGSVRKVFELTKLTSFFEIYDS
                     EADAVAAFK"
     gene            complement(978..>1834)
                     /gene="tgt"
                     /locus_tag="PLG78_19700"
     CDS             complement(978..>1834)
                     /gene="tgt"
                     /locus_tag="PLG78_19700"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNE25404.1"
                     /translation="MFSLSDRMQLDDDGVNFKSHLDGSEHRFTPESAIDIQTDLRSHI
                     MMVLDDCPPADADESRIQASLVRTHSWARRSIDHHNKLCEDGRIDPNRSRMFGIVQGG
                     TSQSARAQSLQAIGELPFSGVAVGGLSVGESRESFYDVLNFLGGRMDPARPRYLMGVG
                     TVPDFIEAVRNGFDMFDCVLPTRNARNGQGLTSQGKLRIRNQSYKNDETPLDPACKCR
                     VCRRYSRAYIRHLFMSDEMLGPQLMSFHNLAFFYTFMEEMRGSIRAGRFATFYLQWKE
                     WDKNGPDE"
BASE COUNT          449 a          478 c          449 g          458 t
ORIGIN      
        1 gaatttgcga tacgcctgac gatatgtgtc gagctgaaca tcgcctgtgg ctgcctttgc
       61 ctgaccctgc gcagtagttc cttctccgta cagcttcttt gcggcggaga aatcatcttt
      121 tgcgagctct tccgcataag cctggtctgc ctgagcgata gtcttttctg ccgtagcgcg
      181 agttgcttct gtatatttgg gagaagcctc tacgcgagca gcgcgagcct gattgaatga
      241 atcaattgcc ttttgctttg cgttgtcata ttcttccgac ccaagcttct ggtgagaagc
      301 aagcaacgca tcacgcgcgc cctggaagtt agaagcagaa tattccggag cgtgctcctc
      361 ttctgccatt gcaatttcat cacgtgcctg tgctaactcc tgcaccggaa caggcttgtt
      421 gcagtacagc aagcccacac cagccagaag aatcaaagcg ggattaattt tggagtgcat
      481 aggttctctc cttgagtgaa tgcgcccgaa gagcgcgcgg catcacttga acgccgcgac
      541 tgcgtcagct tcggagtcgt agatctcgaa gaatgacgta agcttcgtca gttcgaacac
      601 cttacgaacg gagcccgaaa cattgatgat cttgagcccg ccctgatatt tcttcaggtt
      661 ggaaagactg gagatcagag caccaatgcc agacgagtca aggtaagaga cacggtccag
      721 gttgatgatg acgttgtact tctgctcttc gatgagtttg ttgatgagtt ctttgatttc
      781 cggggcatta tagagatcga tctccccgtt gacgtccaga atgacaatgt caccgctagc
      841 tcttcttgtt atttccatat ggttgagaca ggctcctcta tttgtccgga atcctcgaaa
      901 gataccggac tttccacgat ccctgtcaac ataaaaaatt caagcgcaaa ggggaaaaat
      961 gagcaaagaa gtttcagtca ctcatctgga ccatttttgt cccattcctt ccattgaaga
     1021 taaaaagtgg caaaccgacc ggctcgaatc gaccctctca tctcttccat gaatgtgtaa
     1081 aaaaaggcca gattgtgaaa cgacataagc tgggggccca gcatttcgtc tgacatgaac
     1141 aaatgccgta tgtacgcgcg cgaatatcgc ctgcaaacac ggcatttaca tgcaggatcg
     1201 aggggggttt cgtcattttt gtatgactga ttgcgaatgc gaagcttccc ctgtgacgtg
     1261 aggccctgac catttctggc atttcgcgtt ggcaaaacac aatcgaacat gtcgaagcca
     1321 tttcgcactg cttctatgaa atcgggaact gttccaacac ccattaagta tctcgggcga
     1381 gccgggtcca tacgaccgcc aagaaaattc aaaacatcgt aaaaagactc tcgcgactct
     1441 cctacggaaa gcccgcccac agcgactccg ctgaaaggaa gttccccgat cgcctgaaga
     1501 gactgcgctc gggcggactg cgatgtccct ccctggacaa tcccgaacat tctgctacga
     1561 ttcgggtcaa tgcggccgtc ttcgcagagt ttgttgtgat ggtctataga tcgtctagcc
     1621 cagctatgag tacgaaccag agaagcctgg atgcgcgatt cgtccgcgtc cgccggcgga
     1681 caatcgtcca gaaccatcat gatgtgcgaa cgcaagtctg tctgaatgtc tatggcagat
     1741 tctggagtga agcgatgttc gcttccgtcc agatgacttt tgaaattcac accgtcatca
     1801 tccagctgca tgcgatcaga aaggctgaac atct
//
LOCUS       DAOQJL010000687         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_54859_length_1828_cov_4.729836, whole genome shotgun sequence.
ACCESSION   DAOQJL010000687 DAOQJL010000000
VERSION     DAOQJL010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54859_length_1828_cov_4.729836"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLG78_19705"
     CDS             complement(<1..585)
                     /locus_tag="PLG78_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE25405.1"
                     /translation="MEENPQIVRTDIQGALQKGLRIVPVEIDNQMKEAYLDYAMSVIV
                     GRALPDARDGLKPVHRRILYAMHARAWRNDRPFVKCAKIVGEVIGNFHPHGDVAVYDT
                     LVRMAQDFAMRQPLIDGQGNFGSIDGDSPAAYRYTEARLSKIASELLNDIDKSTVDFS
                     PNFDDTRQEPQVLPAAFPNLLVNGSTGIAVGMATN"
     gene            629..880
                     /locus_tag="PLG78_19710"
     CDS             629..880
                     /locus_tag="PLG78_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25406.1"
                     /translation="MRGGGRLAGLNLEGGRLVFLGTGRFFALPGRAAFGAFFTCFLPL
                     SVLDLERKPLFLAIMSSWGAKGILRPSSQRKTEKGGKTG"
     gene            938..>1828
                     /locus_tag="PLG78_19715"
     CDS             938..>1828
                     /locus_tag="PLG78_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNE25407.1"
                     /translation="MRIGVIGHGISIERENLDQSTEDLVFVTVKKALRETGLTIEEID
                     TIVQAGDDIMDGIAINHVYTVEPAGSFLKDESKVERDGAWAVHYAMARLLSGKFRTAM
                     VVAFSKASQCTYSAFSGMSADPFYLRPVGADGDSIAALQANYFMQRTGATEADLAQVA
                     RKNRANGSANQSRTMEGETKDFSIDEILKSARIATPITRLTAAYPGDGCVVMILATEE
                     YVKEKKRNAAWIIGVGLSGDAYYPTYRNLSELKHVGLAANKALEMSGRKIGDVNIFEL
                     QENYAHQELMLCEALGIAPAG"
BASE COUNT          440 a          457 c          508 g          423 t
ORIGIN      
        1 gttcgtggcc atgcccactg caattccagt ggaaccgttg accagaaggt tggggaaagc
       61 agcagggaga acctgcggtt cctgtcttgt gtcgtcaaag ttggggctga agtcaaccgt
      121 gctcttatca atgtcgttga gcagttctga agcgatcttg gagaggcgcg cttccgtata
      181 acggtaagca gccggactgt ctccatcaat cgatccgaaa ttcccctgac cgtcgatgag
      241 aggctgtcgc atggcgaaat cctgtgccat gcgcacgaga gtatcgtaca cggccacgtc
      301 accgtgcgga tgaaagttac caatgacctc acctacgatc tttgcgcact tgacgaatgg
      361 cctgtcattg cgccaggcgc gcgcgtgcat cgcatagagg atgcggcggt gcacgggctt
      421 gagaccgtct cgagcatccg gaagtgctct gcccacaatt acgctcattg cgtaatcgag
      481 gtatgcctct ttcatctggt tatcgatttc taccggaaca atacgaagac ctttctgcag
      541 cgcaccttga atgtctgtgc gaacgatttg tggattttcc tccatccttc ttgcccttct
      601 tgtttttttg gcgtttccgc gctgtttggt gcgcggtggc ggccgtttag cagggctcaa
      661 cctggaaggc gggcggctgg tcttcttagg aaccgggcgc tttttcgctt tgccagggcg
      721 cgccgctttt ggcgcttttt ttacctgctt tttaccttta tctgttttgg atttggaacg
      781 aaaaccgctt ttcttggcca ttatgtcctc ttggggggca aagggcattt tgaggccttc
      841 cagtcaaagg aaaacggaga aaggcggcaa aaccgggtaa gaaaaggccg cggacggaag
      901 cacttgacac cgtgcgtccc ccactcctcc tgcaaatatg cgaattggcg taatcgggca
      961 cgggatttcc atcgagagag agaatctgga ccagtccacg gaggacctgg ttttcgtgac
     1021 tgtgaaaaag gcgcttcgcg aaacagggct gaccatcgaa gagatagaca cgattgttca
     1081 ggcaggcgac gacatcatgg acggaatcgc catcaaccac gtctacacag tggaaccggc
     1141 cggttcattt ctaaaagacg aatccaaagt agagagagat ggagcgtggg ccgttcatta
     1201 cgcgatggct cgtcttctca gcggaaaatt tcgcactgcc atggtagttg cattttctaa
     1261 agcaagtcaa tgtacgtatt cagcgttcag cggaatgtcc gcggatccat tctatctcag
     1321 gccagtgggc gcggatggcg atagcatcgc tgcgctccag gcaaattact tcatgcagag
     1381 gaccggcgca acggaagcag atctggcgca agtggcccgc aagaacagag caaacggatc
     1441 cgcgaatcag tcgcgaacca tggaagggga aacaaaagat ttctccattg atgaaattct
     1501 aaaatctgcc aggatcgcca ctccgattac gcgattgact gcggcctatc caggagatgg
     1561 ttgcgttgtc atgattctcg caacggaaga atacgtgaaa gaaaagaaaa gaaacgctgc
     1621 atggatcata ggtgtaggac tgagcgggga tgcgtattat cccacttaca gaaatctttc
     1681 tgagctcaaa cacgtgggcc tcgcagccaa caaagcactc gagatgtcgg gcagaaaaat
     1741 aggcgatgtg aacatttttg aacttcaaga aaactatgca caccaggaac ttatgctgtg
     1801 cgaagccctg ggcatcgcgc cagccgga
//
LOCUS       DAOQJL010000688         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_54924_length_1827_cov_5.369074, whole genome shotgun sequence.
ACCESSION   DAOQJL010000688 DAOQJL010000000
VERSION     DAOQJL010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54924_length_1827_cov_5.369074"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLG78_19720"
     CDS             complement(<1..646)
                     /locus_tag="PLG78_19720"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNE25408.1"
                     /translation="MKDLEAFTEIRIDLSQESAQALVMLVDDSTIPGLEGYFETLYEE
                     GRRQDSSTLFFYFDPEDSEAAIKMELLLEVIGADTFAIQEKLISKKEYLESYKEHYRA
                     FSIACVGIVPSWKKGEEPEDFKDKSILYLDPGLAFGTGLHPTTRMCIRWFCEQGVRDL
                     RIIDAGCGSGILSLAALLLGARAVLAFDIESNAVRATQQNLALNPGIDSTRLSIL"
     gene            complement(686..>1827)
                     /locus_tag="PLG78_19725"
     CDS             complement(686..>1827)
                     /locus_tag="PLG78_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25409.1"
                     /translation="AISFLKKLYYLRPYQETYLVAAQAAIELQEKLEKKPSNLYAAKK
                     KKIRQEWRTLMDDIFPLLVLLLQRMELKKVEPGTWLFEEIIQANPDERPGRRRAGEPI
                     SDLQAKQAEQKAAEEKQEDPDEEKIEDAVLEDGEEKPASDSKETEIAYGMTLMSQIPI
                     ENMKRKFDVRGEWKLMQNRDKVFLAYLFFKEFEDEYSMILTSSKVRLNANYGGGVKID
                     YRQKLADAYETARSAHDQFRKYVQENMEYRRAANDPVAHTNYVEHAKRVQLIEARRGA
                     TGRQVRQMIKDVMLRVTSNLEVILDDLKGSNQIVGNPDEFFRFDISGATQRKLEGKRI
                     RNCFSETYCYALALKQRLENGDLFGGVIDMPDDEFQRSFPGQSPG"
BASE COUNT          398 a          476 c          437 g          516 t
ORIGIN      
        1 ggagaatgga gaggcgggtg gaatcgatgc ccgggttcag ggccagattc tgttgtgtgg
       61 cgcgaactgc attggactca atgtcgaagg cgagaacggc cctggcacca agcagcaaag
      121 cggccaggct gagaatgccg gacccacagc cagcatcgat gatgcgaaga tcgcgcacac
      181 cctgttcaca gaaccagcga atgcacatcc tggtggtagg gtgcagacct gtgccgaatg
      241 cgaggccggg gtccaggtag aggatcgatt tgtctttgaa gtcttctggt tcttctccct
      301 ttttccagga cggcacaatg ccgacgcagg caatggagaa tgcgcggtaa tgttctttgt
      361 agctttccag atactctttt ttgctgatca gtttttcctg aatggcgaac gtatccgccc
      421 cgattacctc aagcagaagc tccatcttga tggcagcctc gctatcctca ggatcgaaat
      481 agaagaacag agtggatgaa tcttgccttc ttccttcctc gtaaagggtt tcaaaataac
      541 cctccaaccc cggaatcgtt gaatcatcta caagcatgac cagagcctgt gccgattcct
      601 ggctcagatc gattcgaatt tctgtgaatg cctcgagatc cttcaaagac tgttgcggat
      661 ttcctttcaa ttccccggtt ccgtcttatc cgggtgactg ccctgggaag ctgcgctgga
      721 attcgtcgtc tggcatgtcg attacgccgc cgaacaaatc cccattctcc agtctctgct
      781 tgagagcaag agcatagcaa tacgtttcag aaaagcaatt gcggatccgc ttcccttcga
      841 gcttcctttg agttgcgcca gaaatatcaa aacggaaaaa ctcgtccgga ttcccaacga
      901 tttgattgga tcctttcagg tcatcgagta tcacctcgag attgcttgta acgcgaagca
      961 tgacgtcttt gatcatctgg cgcacctgcc ggccggtggc tccgcgtctt gcttctatga
     1021 gttgcactct ctttgcatgc tccacgtagt tcgtatgcgc cactggatcg tttgcagctc
     1081 tgcgatactc catgttttcc tgaacgtact ttctgaattg atcatgcgca ctgcgggctg
     1141 tttcgtacgc gtccgcaagt ttttgacgat agtcgatctt gaccccacca ccgtaatttg
     1201 cgttcagccg aaccttggag gaagtcagaa tcattgaata ttcgtcttca aattctttga
     1261 aaaacaaata cgcaagaaac actttatccc gattctgcat gagcttccat tctccgcgta
     1321 catcgaactt gcgcttcatg ttttcaattg gaatctgact catcagagtc ataccatacg
     1381 cgatctcagt ttctttgctg tcagacgctg gcttttcttc tccgtcctcg agtacggcgt
     1441 cttcaatctt ttcttcatca ggatcttcct gtttttcttc cgcagccttc tgttcggcct
     1501 gctttgcctg aagatcagaa atgggttctc ctgcgcgacg acgaccaggc cgttcgtctg
     1561 gatttgcctg gatgatttct tcaaagagcc acgtgcctgg ttctactttt ttcaactcca
     1621 ttctttgaag gagaagcacg agcagtggga aaatatcatc catgagagtc cgccattcct
     1681 ggcggatctt cttctttttt gctgcgtaga gattcgatgg ctttttttcc agtttttcct
     1741 ggagttcaat ggccgcctgg gccgcaacca gatacgtttc ctgataaggg cgcagataat
     1801 aaagtttctt taggaatgat atggcgg
//
LOCUS       DAOQJL010000689         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_56925_length_1794_cov_3.837263, whole genome shotgun sequence.
ACCESSION   DAOQJL010000689 DAOQJL010000000
VERSION     DAOQJL010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56925_length_1794_cov_3.837263"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLG78_19730"
     CDS             <1..302
                     /locus_tag="PLG78_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25410.1"
                     /translation="RDLAGYLDNSPVAIVATGPEGYCFFCGCPTGNASAWFSDGEWIW
                     PTGIGHCILWHNLRLPDSFVAHIESMNFVPPDKYLTPDKELPWPTRADLSHFQLR"
     gene            880..>1794
                     /locus_tag="PLG78_19735"
     CDS             880..>1794
                     /locus_tag="PLG78_19735"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25411.1"
                     /translation="MKSDPSSILSDLLTKLEKFEKLRSDLAAQIETQATDKLNICESI
                     HASFSGSWMGYHAYLYFHQFQRPPHNYRFDPSDVGFRGLPNGWSEIDYPQMTAYIDSI
                     SKSGSLHSLLGTANKLRKAAEDFQDYAATHLSWIRKRKDFKAEIALLEEIESLKVSVN
                     DQQLLRLLAPGQHMTTDVQAVTQGIKTPPHINYQVKAMHCATIAAQTEELLKKVQRIH
                     TQITLSLDLTEAEGSAETISSITRICSNFQSVARQLQSRRENRPIMEMNDEYDVQDLF
                     HALLRLYFSDIRAEEWTPSYAGKSARTDF"
BASE COUNT          453 a          486 c          406 g          449 t
ORIGIN      
        1 gcagggacct ggcaggctat ctagacaatt ctccggttgc gatcgtggcg acaggtccgg
       61 aaggctattg ctttttttgc gggtgtccca caggcaacgc tagtgcatgg ttctcagacg
      121 gagaatggat atggccgact ggcataggcc attgtatcct ctggcacaat ctgcgcctgc
      181 cggactcgtt cgttgcacat atcgaaagca tgaatttcgt cccgcctgac aaatatctga
      241 cacctgacaa agagcttccc tggcccacgc gcgcagatct gagtcatttt cagcttcgtt
      301 aaggcagttg ttcgtttcct cgccagaagt ctgcgcgccc atccgcacct gcgcataact
      361 taacgtatga ccgttatgcg ccatacctgc cccagccaag caatactcca agcacattgc
      421 taatgacacc ggttagagaa atgttcaaat tacctaattc ttttcgccgc ctggtgattg
      481 ttttcgtagt attggttccg tttgcgcact gttcccagga tggtccatcg ccgcgcggct
      541 atgcaaaagc acaatgcaat caatatcttt tcatactact tacacagtgc acacggactt
      601 ccagttccag ctgttcgtcg gactactttg ccggtgtcac ttctctgatg tattactgct
      661 ttgtcgaagc tgagaaactt gggaactcca agaccgattg cacggatggt aactttacga
      721 atgagtatgt cagcggcttc aagcgtcttt tcgacgatca tcaccgcgga cggggtcagt
      781 gcatgggcca ccagctctga gtgggactaa ctgcttgggg caggtacggc gcataactcc
      841 cgctactacg ttatgcgaaa gtctccggcc aaaacatcta tgaaatcaga cccttcatca
      901 atcctttccg acctattgac gaagctggaa aagttcgaaa agcttcgcag tgatctcgct
      961 gcgcaaattg aaacccaagc gacagacaaa ctgaatattt gcgagtccat tcatgcgtcc
     1021 ttcagcggtt cgtggatggg ctatcacgcc tacttgtatt ttcatcaatt ccaacgccct
     1081 ccccataact atcgattcga tccaagtgac gttggatttc gcggacttcc aaacggctgg
     1141 agtgagattg attatccaca aatgacagcc tacatcgatt caatatctaa atcaggctct
     1201 ctgcactcgt tgcttggaac agcgaacaag cttcgcaagg ctgcggagga tttccaggac
     1261 tatgcggcta ctcatctatc gtggattcgg aaaaggaagg atttcaaagc cgaaatcgct
     1321 cttcttgagg agattgaatc actcaaagtt tcagtcaatg atcagcaact cctcaggttg
     1381 cttgccccag gtcagcatat gacgacagat gtccaagccg ttacgcaggg catcaagact
     1441 cctcctcata tcaattacca ggtgaaagca atgcattgtg caactatcgc cgctcaaaca
     1501 gaggaactgc taaaaaaagt tcaacgcatc catacgcaga taaccctctc gctagatttg
     1561 acggaagccg aaggttctgc ggaaacaatc tcctctatca cgcgcatctg ctccaacttt
     1621 caatccgtcg cccgccaact tcaaagcaga cgtgaaaatc gccccataat ggaaatgaac
     1681 gacgagtatg acgttcaaga tttgtttcac gccctcctga ggctttactt ttccgatatc
     1741 cgtgccgaag aatggacccc gagctacgcg ggcaaatcag ctcgtacaga tttc
//
LOCUS       DAOQJL010000690         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_56971_length_1793_cov_4.195052, whole genome shotgun sequence.
ACCESSION   DAOQJL010000690 DAOQJL010000000
VERSION     DAOQJL010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56971_length_1793_cov_4.195052"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..968)
                     /locus_tag="PLG78_19740"
     CDS             complement(117..968)
                     /locus_tag="PLG78_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25412.1"
                     /translation="MESVVGVLVHQGDRMYLSGEDGRRHFEDDPVWPGYAIHWTGRRL
                     KGRLLPQIDYATGKKMVILWPDEPPARPSVELIYNERLTHYWESMMGHIAINVNGEIF
                     NFSNLVNENETMTPEEYYYRPALGEFAPHPVRGKIGTDEEGRPYYDKFGRLFMRSVHI
                     LRIFDIDPQVVRNHLQEVLKEIHAAPRDPKKPENYAQFSVLKRSCATYTRDALRAAGL
                     KKIAGIMPRDLFVNAAYHTTVKRHGSAEIIQKPQLVVPEAPRSAPTALLNPLNRLRNS
                     SISYKPQ"
     gene            1053..>1793
                     /locus_tag="PLG78_19745"
     CDS             1053..>1793
                     /locus_tag="PLG78_19745"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNE25413.1"
                     /translation="MRFAVWILIAASLPLNARVLDVTAINESPPESFVGSLEDAGGAL
                     TYEQVRAMPETAWVQSRANDLNFGITSSAIWARLDLTSRTPRDLYLIIRAPLLGRVTL
                     FEGERVRELGAEKPFSSREIFHRYFIFPIHIEKQTTLYLRVQTKGLVGIPVRIMTPAA
                     FAQSDWIETLLLGIYYGTMLCMLVYNLFVLVIIRERSYVSYLLYVSAGTVYALSTNGL
                     AFEFLWPNTPALTYRFNGPWVCLYIALSL"
BASE COUNT          404 a          511 c          422 g          456 t
ORIGIN      
        1 cagtcaccgc tccgctttac tcagactggt cttcgcgcct ccgatccatt cgacccgggt
       61 ggatgaacga acaaatgcca atcaaccacg ccgtgaaaga agctttcgaa tttcgtctat
      121 tgcggtttat aggaaatgga tgaattgcgc aaacgattga gtggattgag taaagcggtg
      181 ggcgcgcttc gcggagcttc aggaacaacg agttgcggct tctgaatgat ttccgcagat
      241 ccatgccgct taacggtggt atgataggca gcattcacaa atagatcgcg cggcatgatt
      301 ccggcgatct tcttaaggcc cgcggctcga agggcatctc tggtgtaagt cgcacagctt
      361 cttttgagca cactgaactg cgcataattc tctggtttct ttggatccct gggtgctgca
      421 tgtatttctt tcaaaacttc ctgtagatgg ttgcgaacaa cctgcggatc aatatcaaag
      481 atgcgcagga tgtgcaccga tcgcatgaac agtctgccaa acttgtcata gtagggcctg
      541 ccttcttcat ccgttccaat ctttccgcgc actggatgcg gagcaaactc acccagcgcg
      601 ggacgatagt aatactcctc aggtgtcatg gtttcgtttt cattcacaag attggaaaag
      661 ttgaatatct ctccattgac gttgatggcg atgtgaccca tcatagactc ccagtaatga
      721 gtcagccttt cattgtaaat cagctccacg ctcggacgcg ccggaggttc atccggccac
      781 agtatgacca ttttcttgcc ggtagcgtag tcgatctgtg gaaggagcct gcccttcaag
      841 cgcctgccgg tccagtgaat cgcatacccg ggccagacag gatcatcttc aaaatgacgt
      901 cgaccatcct ctccgctgag atacatcctg tcgccctgat ggactagaac gccgactact
      961 gattccatac tcaatgtctg gtgcggttgc agcctctgtc aaacagaacg aaaattgctg
     1021 ttcagacgtt tcgcctcgct cacaatccgt ccatgaggtt tgcggtctgg atactcatcg
     1081 ccgcctcttt gcccctgaac gctcgagttc tggacgtaac ggcgatcaac gaaagtccgc
     1141 ccgaatcatt tgtaggctca ctcgaagacg caggcggagc actcacctat gaacaagtcc
     1201 gcgccatgcc ggaaactgcc tgggtccaat cgcgcgcaaa cgacctgaac ttcggaatta
     1261 cttcttctgc catctgggcg cgcctggatc ttacgtcacg cacccctcgt gacctgtacc
     1321 tgatcattcg cgcccctctt cttgggcggg taacgctgtt cgaaggcgaa cgcgtacgcg
     1381 aactcggcgc ggagaagcca ttctcttcgc gcgaaatctt tcacagatac tttatctttc
     1441 ccattcatat agaaaaacaa accactcttt acctgcgcgt gcaaacaaaa gggctcgtgg
     1501 gaattccagt tcgcatcatg actcccgccg cattcgcgca atctgactgg atcgaaactt
     1561 tgctactcgg gatctactac ggcaccatgc tttgcatgct ggtctacaac ctctttgttc
     1621 tggtgatcat acgcgaaaga agctatgtca gctatttgct ttatgtttcc gcaggcaccg
     1681 tatacgccct ctccacaaac ggactcgcgt ttgaatttct ctggccaaac acgcctgccc
     1741 tcacatatcg cttcaacgga ccctgggttt gcctctatat cgctctatcc ctt
//
LOCUS       DAOQJL010000691         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_57098_length_1791_cov_3.935484, whole genome shotgun sequence.
ACCESSION   DAOQJL010000691 DAOQJL010000000
VERSION     DAOQJL010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57098_length_1791_cov_3.935484"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PLG78_19750"
     CDS             <1..1788
                     /locus_tag="PLG78_19750"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25414.1"
                     /translation="EFTLRNTTNRSMSVELSARISASAGEGPVVPAQKIDLSAGEARE
                     VGWDINVPAGISSLTYDVQAVEAGGNARERVKIVQKVIEAIPVRTFQATLEQIDKEYV
                     VPVERPKDAIASRGDLRVNLSPSLTGSLEGVREYMKKYPYTCMEQRVSRSISLRDPKM
                     WAETMERLPLYLDSEGFVKFFPEMRFGSELLTAYILAISAESGYEIPAGPREKMIQAL
                     QAFMDGKISRQCPLNTTDLSLRKLTIIEALSRYGESKPQMLETIQIQPNLWPTSAVID
                     WYNILERTPSIVGRTDRMKEAQQILRSRLSFQGTTMSFSTEGNDYMWWLLTSPDMNAI
                     RMTLAVMKNPDWRADLPRIMRGTIGRQRKGSWDLTLANAWGVLAVEKFASEFERITVG
                     GTTSASLGDQTRKLDWSKARGGDLILPWPANRTNMSVRHDGAGKPWALLQSRAAIPLK
                     EDLHAGFRIKKSWKMIEQKKSGIYSRGDIVRVTLKITADADRTWVVVSDPVPAGATIL
                     GTGLGTESSLMQQGEKREGWAWSAYEERSFEAFRSYYEYAWKGEWTVEYTMRLNQSGT
                     FQLPQTRVEAMYSPEMFAESPNEVLSVMP"
BASE COUNT          458 a          402 c          510 g          421 t
ORIGIN      
        1 gaatttactt taagaaatac aactaatcga tctatgagcg ttgagctgag cgccaggatt
       61 tccgcctctg ccggagaagg cccggtagtg ccggcgcaaa agattgacct gagcgccgga
      121 gaggcgcggg aagtgggctg ggacatcaac gtcccggcag gaatctcaag tctaacgtat
      181 gatgtgcagg cagtggaagc tggaggcaat gcccgcgaac gagtgaagat tgttcagaaa
      241 gtaattgagg cgattcctgt tcggacattc caggccactc tggaacagat cgacaaagag
      301 tacgttgttc cagttgaaag gccaaaggat gcaatcgcta gcagaggaga tcttcgcgta
      361 aacctgagtc cttctctcac cggaagcctg gaaggtgtgc gcgaatacat gaagaaatat
      421 ccgtatactt gcatggaaca acgagtctct cgttccatca gtttgcgcga tccgaagatg
      481 tgggcagaga cgatggagcg attgccgctg tacctggatt cggagggatt tgttaagttc
      541 tttcctgaaa tgcgatttgg cagcgagctt ttgactgcct atatccttgc catttccgct
      601 gagtctggtt atgaaattcc agccgggccc agagagaaaa tgattcaggc tcttcaagcg
      661 ttcatggatg gaaagatttc cagacaatgc cctttgaata caacagacct gagtcttcgc
      721 aagctaacca tcatcgaagc actttcgcgt tacggagaat caaagccgca gatgcttgag
      781 accattcaga ttcagccgaa tctatggccg acttctgcag tcattgattg gtacaatatt
      841 ctggagagaa cgccgtctat cgttggtcga acagatagaa tgaaggaggc gcagcaaatt
      901 cttcggagtc gtctgagctt ccagggaacc accatgtcct tttctacgga aggaaacgat
      961 tacatgtggt ggcttttgac atcaccggat atgaacgcga ttcgaatgac tcttgcagtg
     1021 atgaagaatc cggactggcg cgcggatctt cccagaatca tgcgaggtac gattggtcgc
     1081 cagagaaaag gttcctggga tctaactctg gcgaatgcct ggggtgttct ggctgttgaa
     1141 aaatttgcct ctgagtttga gcgtataacg gtaggtggga ccacttccgc ttctcttggg
     1201 gatcagacca gaaaactgga ctggagcaag gctcgtggcg gagacctgat tcttccctgg
     1261 cctgcgaatc gaaccaacat gagcgtgcga catgacgggg cagggaagcc atgggcgtta
     1321 ctgcagagta gagctgccat tccattgaaa gaagatctgc acgctggctt tcggatcaag
     1381 aaatcctgga agatgattga gcaaaagaaa tcaggaatct atagccgtgg cgatatcgtg
     1441 cgcgtcacac tgaagatcac tgcagatgcg gatcgaacgt gggtggttgt ttccgatcct
     1501 gttcctgccg gcgctacgat tcttggcacg ggacttggta cggaatcgag cttgatgcag
     1561 caaggcgaaa agcgggaagg atgggcgtgg tctgcatacg aagaaaggtc ctttgaggca
     1621 ttccgttctt attacgaata cgcgtggaaa ggcgaatgga ctgtcgaata caccatgcgc
     1681 ctgaatcaat ccggtacttt tcagcttccg cagacgcgtg tggaggccat gtattctccg
     1741 gaaatgtttg cagaatcgcc taacgaagtg ttaagcgtta tgccttgagc g
//
LOCUS       DAOQJL010000692         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_57570_length_1784_cov_3.543088, whole genome shotgun sequence.
ACCESSION   DAOQJL010000692 DAOQJL010000000
VERSION     DAOQJL010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57570_length_1784_cov_3.543088"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="PLG78_19755"
     CDS             complement(<1..1728)
                     /locus_tag="PLG78_19755"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE25415.1"
                     /translation="MRRAFYFAALTLLFGACSDRSVVDLTGKWRYRMGFETAYLASRE
                     DPAWKEIALPAFLYKTLNLQDYRGWITLQKDLPSSMSILEETPGLVRMGWNSDVAVFY
                     IDDQRIGQTGSVEPYESGAYRPSAKTFSVLSHRDTHTVTMALYTNGQFPLQVFGPEME
                     IGPADVLRQRMDVAQVISFSFLAIYFIVGAYHLLLFSVRRRDRHNLFFGIFCMLLTVY
                     WFFRTDFRGSLFGSNVLYQVKTEYSTLFMIGPSMIFFLSQYFEKKFSRIGIVVTVFWT
                     GLLLWLLSATAYQTTADVLRVWQLTALPTMLFVVFYMGREAFRGNRDARWMLAGFVVF
                     FLFVLHDLLSSLFQIDTPHLARYSFIIFVLGIAGVLARRFTRIHNQVEELNEHLEEKV
                     RERTDQLHKTLHAVEELKVQQDGDYFLTSLLIQPLTGNFVEGSAVSVDIVTRQKKQFR
                     FKHWEAEIGGDLATANVITLRGRRYAVFINGDAMGKSIQGAGGALVLGTVFKSILSRT
                     QVSSYFQNRYPEQWLKEMFLELQSVFTAFDGSMMVSAVFGLVSEESGLVYYVNAEHPF
                     VVLYRRGKAS"
BASE COUNT          518 a          455 c          427 g          384 t
ORIGIN      
        1 gctcgccttc ccacgacgat acaacacaac aaatggatgc tccgcgttga cgtaatacac
       61 aagaccgctt tcctccgaca ccagaccgaa cacagccgat accatcatac tgccatcgaa
      121 ggcggtgaag acgctttgca attcgaggaa catttctttg agccactgct ccggatatcg
      181 attctgaaaa tacgatgaca cctgagttct agagagaatg gacttgaaaa ccgtaccaag
      241 aacgagggcc ccgccagcac cctgaatcga tttacccatg gcgtccccat ttatgaatac
      301 cgcataacgt cttccgcgaa gagtgatcac attggcagtg gcaagatctc cgccgatttc
      361 cgcctcccag tgtttgaagc gaaactgctt cttctggcgg gttacaatat ccacgctcac
      421 agcgctccct tctacaaaat ttcctgtcaa aggctgaatc aagagggaag tcaaaaaata
      481 gtctccgtct tgctgcacct tgagctcttc tactgcgtgg agagtcttat ggagctggtc
      541 cgttctttcg cgaacttttt cttccagatg ctcgttgagc tcttctacct gattgtggat
      601 tcgcgtaaat ctacgcgcga gaactcctgc aatcccgagg acaaaaataa tgaacgagta
      661 acgtgctaaa tgaggcgtat cgatctgaaa cagagaactg agtagatcgt gcaaaacaaa
      721 aagaaagaac actacaaagc cagcgagcat ccacctggca tcccgattgc cgcgaaatgc
      781 ctctcgaccc atgtaaaaaa ctacaaagag catcgtagga agagcggtga gctgccagac
      841 tctgagcacg tctgctgttg tctggtacgc cgttgcagag aggagccata gaagaagtcc
      901 ggtccagaaa acggtgacca cgattccaat gcgcgaaaac ttcttctcga agtattgaga
      961 aagaaagaag atcatgctgg gaccgatcat aaacagagtg ctgtattctg tctttacctg
     1021 atagagcaca ttggatccaa acagagaacc tcgaaagtca gtgcgaaaga accagtatac
     1081 tgtcaggagc atgcaaaaaa tcccaaagaa gagattgtgc cgatcgcgcc ttcgcacgga
     1141 aaacagaaga agatgatatg cgcctacaat gaagtagatg gcaagaaacg aaaaagaaat
     1201 aacctgagcc acatccatcc tctggcgaag aacatcggcg gggccgattt ccatttctgg
     1261 accaaacacc tggagaggaa actggccatt cgtgtaaaga gccatggtca cagtatgagt
     1321 atctctgtga gataaaacag agaaggtttt cgcggaaggt ctatatgcac ctgattcgta
     1381 tggttccaca cttccagtct ggccgattct ctgatcgtct atgtagaata ccgcaacatc
     1441 cgaattccat cccatgcgaa ccagtccggg agtttcttcg agaatggaca tggaagacgg
     1501 caaatccttc tgcaacgtga tccagccacg atagtcctgt agattgagcg tcttgtacag
     1561 gaaagcggga agcgcgatct ctttccacgc aggatcctct cgcgacgcaa ggtaggccgt
     1621 ctcaaagccc attctgtagc gccattttcc ggtcagatcc accacagatc gatccgaaca
     1681 ggcgccaaag aggagtgtca gcgctgcgaa gtagaaggcc ctgcgcatgg gcagtgttat
     1741 agcacgcaaa cggaaacagt caagcctgta ctgacgttag ttgg
//
LOCUS       DAOQJL010000693         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_57634_length_1783_cov_3.811343, whole genome shotgun sequence.
ACCESSION   DAOQJL010000693 DAOQJL010000000
VERSION     DAOQJL010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57634_length_1783_cov_3.811343"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLG78_19760"
     CDS             complement(<1..336)
                     /locus_tag="PLG78_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25416.1"
                     /translation="MDLAGRILHWLSRILFPIAFIPLLFPYTTVLFIVLVFAAVIVYL
                     GERRSGGLPLTQSRSFHVMRLSSIAVLLNAALFFGLFLYSQTCILRPLSQQLDPLREY
                     YRQQGQTQSE"
     gene            480..>1783
                     /locus_tag="PLG78_19765"
     CDS             480..>1783
                     /locus_tag="PLG78_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25417.1"
                     /translation="MLEFLGRIYWNYFAIGYIVVGAFTLLAGLFAFTIKDRSRATFHF
                     GMFFLAATALSFAFTYGHFFYDTAAVYHRYFTIIGAMSASVHILQFLIHFPEPSKRKV
                     AWFAWVQWIVIALTVGYFAKVTWSIERTFHFSAQLWDYDAEVLSRNVGFVIIVYALIY
                     VVYGVFKVIFKLKGQPRWVVLAMLIAFISVTLVPGILNVLSREGAIDRNLFVNIFTLL
                     NIVGCFTVVILFINNTSDRTTFMVKITGITLVTVLFIFQILTYFSLKGMDENFDEIHF
                     REGELFLKGQRSDSVQYAYTHRLSASMSQEPIQANEKEKDKNKEKEAVPVSGGVATVD
                     YREHEHEYENTYILYTISGLGRDRFNQELETILSRSPVHFNGYSNLIREYLRNAREAS
                     AADVLERIASVQRLVGYRAFKIRQLPDKTFRKDLTEFLKGSGA"
BASE COUNT          445 a          402 c          452 g          484 t
ORIGIN      
        1 ctccgattgt gtctggcctt gttgtctgta gtactctctg agcgggtcaa gctgttgcga
       61 aagagggcga aggatgcatg tctgcgaata caggaaaagg ccaaagaaaa gggctgcatt
      121 cagcagcact gcaatgctgg aaagtctcat cacatggaaa gatcttgatt gggtgagcgg
      181 aaggccgcct gatcgccttt cacccagata gacgatcacc gcggcgaaga ccagcacgat
      241 gaatagaacg gtagtataag gaaagagaag aggtataaaa gcaatcggga acaggatgcg
      301 cgaaagccag tgaagtatgc gacctgccag atccacggga gcagtcagaa ggcctgacga
      361 agaagagcta tccttttttg gccgcgaaat cccacggatg gcaagaaaaa tttcaggatc
      421 gcttgacctg gggcatgact gagccgtatt ctggggagag gggctgtcta tctatcgtta
      481 tgcttgagtt tctgggccga atctactgga actactttgc gattggctac atcgtagtcg
      541 gggctttcac tctgcttgca gggctctttg cttttaccat caaagatcga tccagggcca
      601 cctttcattt tggcatgttc tttctggcgg ccaccgcatt gagttttgcc ttcacttatg
      661 ggcatttctt ctatgatact gcggcagtct atcatcgcta tttcacaatc atcggggcca
      721 tgtctgccag tgtgcacatc ctgcaattct tgattcattt tccagaacct tcaaaacgca
      781 aagttgcctg gtttgcgtgg gttcagtgga tcgtaatcgc gctcactgtc ggctattttg
      841 caaaggtcac ctggtcgatc gagagaacgt ttcatttttc tgcgcagctc tgggattatg
      901 atgcggaggt tctgagccgc aacgttggtt ttgtgatcat tgtctatgca ttgatctatg
      961 tggtgtacgg tgtattcaaa gttattttca aattaaaggg gcagccccgc tgggttgttc
     1021 tggcaatgct cattgctttt atttcagtca ctcttgttcc gggcatactg aatgttctat
     1081 ctcgcgaagg cgcgatcgat cgcaacctgt tcgtgaatat ctttactctt ctcaacattg
     1141 taggttgctt cacggtcgtc atcttattca tcaataacac ttctgatcgc accactttca
     1201 tggtgaagat cactggcatt acactggtaa cggtgctatt cattttccag attctaacct
     1261 acttcagtct caaaggaatg gatgagaatt tcgatgagat ccatttcaga gaaggggagc
     1321 tgtttctgaa aggacagcgc tcagattcgg ttcaatatgc atacactcac cgattgagcg
     1381 cttccatgtc tcaggaaccc atccaggcaa acgaaaaaga aaaagacaag aacaaggaaa
     1441 aagaagcagt cccagtttcc ggaggagtgg ccactgtaga ttaccgggag cacgaacacg
     1501 agtacgagaa tacttatatt ttatatacga tttccggtct ggggcgcgat cgattcaatc
     1561 aagagctgga aacgattctt tctcgcagcc ccgtccactt caatggatat tcaaacctga
     1621 ttcgtgagta tttgagaaat gcaagagagg cctctgcggc ggatgtactg gaacgtatcg
     1681 cgtctgtgca acgtctcgtc gggtatcgcg cttttaagat cagacaactg cctgacaaaa
     1741 cgtttcgcaa ggatctcaca gagtttctga agggttccgg tgc
//
LOCUS       DAOQJL010000694         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_58373_length_1772_cov_3.962143, whole genome shotgun sequence.
ACCESSION   DAOQJL010000694 DAOQJL010000000
VERSION     DAOQJL010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58373_length_1772_cov_3.962143"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1772
                     /locus_tag="PLG78_19770"
     CDS             <1..>1772
                     /locus_tag="PLG78_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNE25418.1"
                     /translation="DNSVDEAMAGYAKHIIVRMLPDGVIEVQDDGRGIPTGIHPTEKI
                     STIEVVMTMLHAGSKFGDGGYKISGGLHGVGVSVVNALSEWLEVEVHRDGQIHVMSFE
                     RGKVKNPAKVKGKTNRTGTTTRFKADNTIFTTTEYKYALLHQRLEEMAYLNAGLKMEL
                     RDERKQPAKKEVFEFAGGIKEFVARHLQKKQKLHKHIIAFEGKNDKLIGEFAMAYTDS
                     QNEHIFCFTNGINNNLGGTHLEGFRTALTRTLNEYVKKDESMKKKLGGTSLTGDDVRE
                     GLVAVISVKVPEPQFNSQTKEKLVNAEVKGLVQQIVGDKLSLFLEENPGEIKPILEKC
                     VLSFRAREAARKARELVTKRKGVLESGGLPGKLADCSERNPELCELFLVEGDSAGGSA
                     KQGRNRHYQAILPLKGKILNVIKGSDDKILENEEIRTLVTAIGIGFGNDGLDLDKLRY
                     HKIIIMTDADVDGSHIRTLLLTFFYKKMRPLVDNSFLYIAQPPLYLIKNGKDAHYAFT
                     DQEKDKIVKKYNSDKTVVQRYKGLGEMNPEQLWETTMDPEKRVMLKVRPLSESEVGLD
                     GIERTFDILMGDDVEPRRRFIEQN"
BASE COUNT          518 a          412 c          461 g          381 t
ORIGIN      
        1 tagataactc agtagatgag gccatggccg gttacgccaa acacatcatc gtgcgcatgc
       61 ttccggatgg cgtcatcgaa gtacaggatg atggccgcgg cattccaacc gggattcacc
      121 caacagaaaa gatctctacg attgaagtgg tcatgactat gctccacgct ggatccaagt
      181 ttggagacgg cggctataag atttctggcg gtcttcacgg tgtgggcgtt tcagtcgtca
      241 acgcgctgtc tgaatggctg gaagtagaag ttcatcgcga tggacaaatt catgtaatgt
      301 catttgagcg cggcaaggta aagaacccgg ccaaagtgaa aggcaaaaca aatcgcactg
      361 gaaccaccac tcgcttcaaa gcagacaaca caatcttcac cactacagaa tacaaatacg
      421 cacttctgca tcaacgcctc gaagaaatgg cgtacctcaa tgccggcctg aagatggaac
      481 tgcgcgatga gcgcaaacaa ccggcaaaga aagaagtatt cgagttcgca ggtggcatca
      541 aagagttcgt ggccaggcat ttgcagaaaa agcaaaagct tcacaagcac atcatcgcat
      601 ttgaaggcaa aaacgataag ctgatcggtg aattcgccat ggcgtacact gattcgcaga
      661 acgaacacat cttctgtttt acaaacggca tcaacaacaa cctgggtgga acacacctgg
      721 aaggctttcg cactgctctg actcgtactc tcaacgaata cgtgaagaaa gatgaatcca
      781 tgaagaagaa gctcggcgga acttctctta ctggagatga cgtgcgcgaa ggtcttgtgg
      841 ctgtgatctc cgtaaaggtg ccagagcctc agttcaactc gcaaacaaaa gagaaactgg
      901 taaacgcgga agtcaaagga ctcgtgcagc agatcgtcgg agacaagctg tcattgttcc
      961 tggaagaaaa tccaggtgag atcaaaccaa ttcttgagaa gtgcgttctt tcttttcgcg
     1021 ctcgcgaagc agcccgtaag gcgcgcgaac ttgtaaccaa gcgcaagggc gtactggaga
     1081 gcggtgggct tccaggaaaa ctggcggact gctctgaaag aaatcctgag ctgtgtgaac
     1141 tcttcctcgt ggaaggggat tctgcgggtg gatctgcaaa gcagggcaga aatagacatt
     1201 atcaggccat acttccactc aaaggaaaga tcctcaatgt gatcaaggga agtgatgaca
     1261 agattctgga aaatgaagaa atcaggactc ttgtaactgc cattggaatt ggttttggaa
     1321 atgatggtct ggacctggac aagcttcgct atcacaagat catcatcatg acggatgcgg
     1381 acgtggacgg ttcgcacatc cgcactctct tgctcacttt cttctacaag aagatgcgtc
     1441 ctcttgtgga caatagcttt ctctatattg cgcagcctcc tctctacttg atcaagaacg
     1501 ggaaggacgc acactacgct tttacggatc aggaaaaaga caagatcgtc aagaagtaca
     1561 attccgacaa aaccgtggtg cagcgttaca aaggtctggg tgaaatgaat ccggagcagc
     1621 tctgggaaac cacaatggat ccagagaagc gcgtgatgct gaaggtgcgt ccactttctg
     1681 aatctgaggt tggcttggac ggaatcgaac gcacgtttga tattctgatg ggcgatgacg
     1741 tggaaccacg cagacgcttc attgagcaga ac
//
LOCUS       DAOQJL010000695         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_58657_length_1768_cov_4.335668, whole genome shotgun sequence.
ACCESSION   DAOQJL010000695 DAOQJL010000000
VERSION     DAOQJL010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58657_length_1768_cov_4.335668"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1021
                     /locus_tag="PLG78_19775"
     CDS             128..1021
                     /locus_tag="PLG78_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE25419.1"
                     /translation="MSQNGKVRTIARIIDSIRQREGGGFIVRRPFPTQSVSYVDPFLL
                     LDEMGPADYAPGEAVGAPDHPHRGFETVTYMLEGEFQHEDSAGHRGFLRPGDVQWMTA
                     GAGIIHSEEPSEKIMREGGRVHGFQIWVNLPSQLKMTQPRYQEVPGSGIPTAQTEDGL
                     ARVRVIAGEALGVRAVIETHIPIVYQDWTLSPGASASVIVPQEQNAMIYVFEGSVAVG
                     DRQNVLEDGQLGVLGLGDTVQLFGTQTGGRMLLLAGVPTSEPVARYGPFVMNTVEELQ
                     TAFNDYQSGKMGEITRGAKYG"
     gene            1014..1454
                     /locus_tag="PLG78_19780"
     CDS             1014..1454
                     /locus_tag="PLG78_19780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE25420.1"
                     /translation="MDNSSITFETIRDPDGDLQEFLWERLREASNPKLSDERAKEHHA
                     LSILAREKGEIVGGMLAIVYFRGMNLQCLWVREDQRGKKLGSAFLEQAEQAARSLDCT
                     LIHGHTFSFQAPDFYLKAGYEVFGTIEDYPPGKNCFFLRKYLRK"
BASE COUNT          435 a          425 c          488 g          420 t
ORIGIN      
        1 gcgcgcggca atcgccatgc aactcttttg cctggctgaa cattttgagc tccgggtaaa
       61 aaagaatggc cattgaaagt aattgaacct tcaatattaa agagaactta taaatgagag
      121 gtccactatg agccagaatg gaaaagttcg tacaattgcg cggatcatcg attcgattcg
      181 acagcgcgag ggaggcgggt ttatcgttcg ccgcccgttt ccaactcagt ctgtcagtta
      241 tgtagatccg tttctacttc tagatgagat gggcccggcg gactatgcac ccggggaagc
      301 agtgggagct ccggatcatc cgcatcgtgg atttgaaacg gtcacctaca tgctcgaagg
      361 cgaattccag cacgaagatt ctgcaggcca tcgcggtttt cttcggcccg gggacgtgca
      421 gtggatgact gctggtgcgg gaatcattca ttcagaggaa ccttcggaga aaatcatgcg
      481 tgaaggcggc agagtgcatg ggtttcaaat ctgggtgaat ctgccctctc aactgaagat
      541 gactcagccg cgttaccagg aagttccggg atcggggatt ccaactgcgc aaacagaaga
      601 tggtctcgcc cgcgtgcgag tgatcgcagg tgaggcactc ggcgttcgcg cagtcatcga
      661 aactcacatt cccatcgtgt atcaggattg gaccctttca cccggcgcat ctgcgtctgt
      721 cattgttccg caagagcaaa atgcgatgat ctacgttttc gaaggaagcg ttgccgtagg
      781 cgatcgccag aatgtgctcg aagacggaca acttggtgtt cttgggctgg gagacacagt
      841 tcaattgttt ggaacgcaaa caggtgggcg catgcttctc cttgcgggtg ttcctacgag
      901 cgagcccgtc gcaaggtacg gccctttcgt catgaatacc gtggaagaat tgcagacagc
      961 attcaacgat taccagagcg gcaaaatggg agagatcact cgcggcgcaa aatatggata
     1021 attcatcgat cacattcgaa acgattcgag atccagacgg agacctccag gaatttctat
     1081 gggaacgcct gcgggaagca agcaatccaa agctttcaga cgaaagagca aaggaacacc
     1141 atgcgctgag catactggct cgtgaaaagg gagagatcgt cggcggaatg cttgcaattg
     1201 tttactttcg tgggatgaac ctgcaatgtc tctgggtacg cgaggatcag cgtggtaaga
     1261 agctcggttc cgcattcctg gagcaagccg agcaggcggc gagatccctg gactgcactt
     1321 tgattcacgg gcataccttc agcttccagg cacctgactt ctatctgaag gcaggctatg
     1381 aagttttcgg gacgattgaa gactatccac ccggtaaaaa ctgctttttt ttacgaaaat
     1441 acttacgaaa atgaacgaca caaaaccagt catgaacagc acacgatggc agatttcggc
     1501 tatcgatttc tgttgttcat gactgaaaag atggtgttca cgtattctct gtcgcgcccc
     1561 tgacgatcgc ctaacgttcg cgaaggtttc gtacgcaaac tgctggacat gtctggttgt
     1621 ttgttagagg aatcacgtat ggcacgctca ctttgcacat ggatgttcgt tctgattgcg
     1681 gtcttttcgg cgtcttgttc ccaacaatcg cacggggggc ccgcggtgga tcccatcttc
     1741 ttgattgcgg ggcttggcac aaatcaag
//
LOCUS       DAOQJL010000696         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_58724_length_1767_cov_4.409463, whole genome shotgun sequence.
ACCESSION   DAOQJL010000696 DAOQJL010000000
VERSION     DAOQJL010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58724_length_1767_cov_4.409463"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLG78_19785"
     CDS             <1..736
                     /locus_tag="PLG78_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNE25421.1"
                     /translation="DSIIAHLESNLSKNDRAQPQKVEQKALERLAETIRTVPDIAPPR
                     GEARAVMLIGPTGMGKTTSLAKLAARYFFYEKREVSMYSLDRYRLAATQQLRMYADVM
                     KLPFHAPMGPSEFREQTSLDPAELLLIDTSGIGYRDVKRLSELQELAEACAVRLEKHL
                     VLSANADESTLEAIFAAYDAVGFDKILLTKLDESEFIGTFIEYADKVNRPFSFVTNGQ
                     DVPADLIGPGPEDLARILLRKEAATR"
     gene            872..1681
                     /locus_tag="PLG78_19790"
                     /pseudo
     CDS             872..1681
                     /locus_tag="PLG78_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
BASE COUNT          477 a          444 c          464 g          382 t
ORIGIN      
        1 cgattcaatc atcgcacatc tagaaagcaa tctctcaaag aacgacaggg cgcagcctca
       61 aaaggtagag caaaaagcgt tggagcgact tgcggaaacg attcgcacag taccagacat
      121 tgcgcctccg agaggagaag cgcgcgcagt catgctcatt ggacccacag gaatggggaa
      181 gaccacgagc ctcgccaaac tggctgcccg atatttcttt tacgagaagc gtgaagtatc
      241 catgtacagc ctggaccgat accgcctggc agccacccag caactgcgca tgtatgcgga
      301 tgtgatgaaa ctgccattcc acgctcccat gggtccctcg gaatttcgcg aacaaaccag
      361 cctggatcct gccgagctgc ttttgatcga cacatctgga attggataca gggacgtgaa
      421 acgcttatcc gagctgcaag agctggcaga agcctgcgca gtcaggctcg aaaaacacct
      481 tgtattgtcc gccaatgcgg atgagtctac tcttgaggca atttttgccg cttacgatgc
      541 ggttggcttt gataaaatct tgctaaccaa actggacgaa agcgaattta tcggcacttt
      601 tatagagtat gccgataagg tgaacagacc gttttcattc gttacaaacg gccaggacgt
      661 cccggcagat ctcattggcc caggcccgga agatcttgca aggatactgt taagaaagga
      721 agctgcgacc aggtaggtgt gatttatgga gcagttggat caagcctcga ctctgcgccg
      781 catggcaaat cagatggatc gaggaagcac tgcggtactg gaaagggaca tggagcacga
      841 aaaacagccg tctcatgaga caccgcagcg cacgaaagtg ctgacggtca cgtccggaaa
      901 aggtggggtc ggtaagacta ccgtgtccgt gaatcttgca atcagtctgg ctcgcgctgg
      961 aaagaaagtt ctgttgtttg acggagacct gggtcttgca aacatcaacg ttcttcttgg
     1021 catcattcca gaacacaaca tctacgaagt actcaaaggc aaaaagaagc ttcaggatat
     1081 catcattcga acgtcatacg gcattgatat cattgctggc gcgaatggaa tctctcaact
     1141 cgcaaacctc agcgattcgc agcgcgaaaa cttcctcgaa ggtctgcaat ctttgatggg
     1201 ctatgacatc atgctcatcg acactggagc aggcgtcggt gcaaatgtga ttggccttgt
     1261 tcttcccgca gatgaaattc ttgtagtaac gactccagag cccactgcaa ttacagacgc
     1321 atacggcatg atcaagagcg ttgtttcgca cgggccaggt ggtaagatca agttgattgt
     1381 gaatcgggtc ccggatgcag gcgaagcgaa aatggtcgca gacagactca tcagcatttc
     1441 caatcaattc ctgaaagcca gagtggaaaa cctcggcttc attttcgaag aaagctcggt
     1501 tcaaaaaagc attcgcagcc agcgaccata tgttgtgctt tttccgaacg cgaagagcag
     1561 cgcctgcatc aaacacatcg ctgctcgcct gatcaatgct ccggtagaag aagatagcgc
     1621 gggcatatat cagttctttc agaaaatgat gaatttcagt tttcgcggca aagacgacta
     1681 acctcccgat atgggcccga tcctgaaatt cacggccggg ttcgccatcg cgggacctcg
     1741 ctcgggtggg ggtgcccaca gggtagg
//
LOCUS       DAOQJL010000697         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_60203_length_1744_cov_3.334517, whole genome shotgun sequence.
ACCESSION   DAOQJL010000697 DAOQJL010000000
VERSION     DAOQJL010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60203_length_1744_cov_3.334517"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1623)
                     /locus_tag="PLG78_19795"
     CDS             complement(322..1623)
                     /locus_tag="PLG78_19795"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE25422.1"
                     /translation="MKKFKFASDFLWGTATAPTQIDGGDNASDWYEFCQQKGRILDGS
                     SSFTGCDHWNRYKEDFDLMKKMGHNAYRLGADWSRFEPQPGAFDHKALDHFREMLQYL
                     KKKKIKPLLTLMHFAVPSWWLKKGGWTKEENIEDYMRFARWLIENTGDLVHEYITINE
                     PVVYSVIAYMDGRWPPGEGGLLGYFRSLTVQRNLALTHFQLYDLIHSAHARRNWKKPA
                     VSIAKHMRVTDPENPASGLDVNAARTVDEFFNLAFCDTIQSGQMSGGLGKGERVHDGK
                     AWDFFGMNYYSRDIVSFDLLRPHQAFTKIQVQPGSPRNDLNWEIYPEGMKRTLMKVHD
                     RYGLPIRITENGIADAKDAQRPQFLIDHISAMESAAQEGVKIDGYYHWSFMDNFEWAE
                     GYTARFGLVEVDFKTMQRKPRASARIFSKIIKNRGTAGIRA"
BASE COUNT          373 a          436 c          436 g          499 t
ORIGIN      
        1 tcattgacag ctgtcttcag attctgaact accttggtga agtcctgtgc cggggagact
       61 tctacttcat aactttttgc attggcgtct ttggcccagc tgagcttgta ctggagtgct
      121 tcagcggaca gcccgctcac ggcaacggcg agcaaaatgg cgataaattt gcgcataggt
      181 tcctcggaat acctttttgg ggaagttttg tcagccagag aatgacgtca actcaggatt
      241 gcatcggcga atggtttttg cccaaacggt ttctgaatcc cgaattatgt cgcattagta
      301 gagccacgat cctggatcag atcaggccct gatccccgcc gtgcccctgt ttttgatgat
      361 cttgctgaag attctggcag atgctctggg tttgcgttgc atggttttga aatccacttc
      421 cacgaggcca aatctggcag tgtaaccttc cgcccattca aagttatcca tgaaactcca
      481 gtggtagtat ccgtcgattt tgactccttc ctgagccgcg gactccatgg cggagatgtg
      541 atcgatcagg aactgtggcc tttgtgcgtc ctttgcatct gcaattccgt tctcagtgat
      601 tcggatgggt agaccgtagc gatcatgcac cttcatcaag gttctcttca ttccttctgg
      661 atagatttcc cagttcaaat cgttgcgagg agagcctggc tgcacctgga tcttggtgaa
      721 agcttgatgt ggtcgcagca gatcaaagga cactatgtcc ctggaatagt agttcatccc
      781 gaagaaatcc catgcctttc cgtcgtggac tcgttctcct ttcccgagtc ctccactcat
      841 ttgcccggat tgaattgtat cgcagaatgc aaggttgaag aattcgtcca cagtgcgcgc
      901 cgcatttacg tccagaccgc ttgctggatt ttctggatcc gtcactctca tatgctttgc
      961 aatggaaaca gcaggcttct tccagtttct gcgtgcgtgc gcagagtgaa tgagatcgta
     1021 gagctggaag tgcgtaaggg cgaggttgcg ctgcacagtc aaagaccgga agtaaccaag
     1081 cagtccgccc tcaccgggtg gccatcttcc atccatgtag gcgatcacgg aatagacgac
     1141 tggttcattg attgtgatgt attcgtgcac gaggtcgcca gtgttttcga tcaaccagcg
     1201 cgcgaagcgc atgtaatctt ctatattctc ttcttttgtc cagccgcctt tcttcagcca
     1261 ccacgagggc actgcgaaat gcatgagagt cagtagaggt ttgatcttct tcttttttaa
     1321 gtattgcaac atctcacgga agtgatcgag agctttgtga tcgaaagccc ctggttgcgg
     1381 ttcaaaacga gaccagtctg cgcccagtct atacgcgttg tgacccatct tcttcatcag
     1441 atcaaagtct tctttgtatc gattccagtg atcgcatccc gtaaacgaag acgatccgtc
     1501 tagaattcta cccttttgct ggcagaactc gtaccagtct gaagcgttgt ctccgccgtc
     1561 tatttgagtg ggcgctgtgg ccgtgcccca gaggaagtca gatgcgaatt tgaatttttt
     1621 cattgtgaac cgcgaagctt ttcaaaaggt agggttgccc gatgagtcaa ccatttatac
     1681 gtatcgcgct tgtttctgtg ctttttttcg tgcatcccgg tttttcggag aatcgcaagg
     1741 cgga
//
LOCUS       DAOQJL010000698         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_61450_length_1726_cov_3.464393, whole genome shotgun sequence.
ACCESSION   DAOQJL010000698 DAOQJL010000000
VERSION     DAOQJL010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61450_length_1726_cov_3.464393"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLG78_19800"
     CDS             <1..535
                     /locus_tag="PLG78_19800"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNE25423.1"
                     /translation="LRGNDSYVRLDDEDSIYLVEENLRTAFGSGDLEYFRNRRVFPDM
                     SKDDITGITASATGRPLQIARNGQGWQMLSPVPGNVNGSEMNLLLGDFVEWKAVSFPT
                     EIPKDIDKKKSLKVEVQFKTTMTEPRTFAFEVLGQKDFSSYIIRTADGNLFEVTSIYL
                     SHLLEPEEKLLERAEKR"
     gene            complement(557..997)
                     /locus_tag="PLG78_19805"
     CDS             complement(557..997)
                     /locus_tag="PLG78_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25424.1"
                     /translation="MRVTVIKNIAKSRVKWIVLSAFVVLSSCVQPPSERPQEPGTPAR
                     VVTYYNDVPQNCSEIKGLKQTVVRRVTSEKELIRHYGMTFRVRFQDKVTPELYGALYY
                     QDAKENKYCMALPDGRFAVFHDSQLAEHTMNAAQEVCYPIMACN"
     gene            996..>1726
                     /gene="dgt"
                     /locus_tag="PLG78_19810"
     CDS             996..>1726
                     /gene="dgt"
                     /locus_tag="PLG78_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNE25425.1"
                     /translation="MFQTPTTSFEEKEDSRFAPYAVKHSLSGGRISEEKPQEERTCFQ
                     RDRDRIIHSKAFRRLGYKTQVFVNSEGDMFRTRLTHSLEVAQLSRSAASYLGLNADYC
                     ETIALAHDMGHSPFGHAGQDILNRLMANHGGFEHNCQTLRIVSALETRTIDWNGLNLT
                     RLSLIGMMKHVRIYECDTALREILDYRRNISPPLEAVLVDTCDRLAYLHHDFEDGLDS
                     GFLDRAEVLSHPAFLHAQRTLQDRHG"
BASE COUNT          422 a          413 c          431 g          460 t
ORIGIN      
        1 tttgcgagga aatgattcat acgttcgcct cgatgatgaa gattcaatct atctcgtaga
       61 agaaaatctt cgcactgcat ttggatcggg ggacctggag tatttcagaa atcgcagagt
      121 attcccggac atgagcaagg acgatatcac cggaatcact gcatctgcta ctggcaggcc
      181 ccttcagatc gcgcgaaatg gccaggggtg gcagatgctt tctccggttc ctgggaacgt
      241 caatggttca gaaatgaatt tgcttctggg cgattttgta gaatggaagg cggtttcctt
      301 tcccactgaa attccaaagg acatagataa aaagaaaagt ctaaaagtcg aagttcaatt
      361 caaaacaacg atgactgagc ctcgcacatt tgcctttgaa gtgctggggc aaaaagattt
      421 cagttcctat atcattagga cagcagacgg aaatcttttt gaggtaactt ccatttacct
      481 gtctcatttg cttgagcccg aagagaagtt gctcgagcgc gctgaaaagc gatagggctc
      541 aggcgccttg cagctgttag ttgcacgcca tgatcggata gcacacttcc tgtgccgcat
      601 tcatggtgtg ctctgccagc tgggaatcat gaaacactgc gaaccttcca tctggaagtg
      661 ccatgcagta tttgttttcc tttgcatcct ggtagtagag cgctccgtac aattcaggag
      721 tcactttgtc ctggaagcgc acacgaaatg tcattccata gtggcggatc agttcttttt
      781 ctgaagtcac tcttcttacg accgtttgct tgaggccttt gatctccgag cagttttggg
      841 gaacgtcgtt gtaataggtt acgacgcgag caggagttcc aggttcctgt ggcctttcag
      901 atggcggctg gacgcaggag ctcagaacga cgaaagcgct caggacgatc catttgacac
      961 ggctttttgc tatatttttg atgactgtga ctctcatgtt tcaaacacct actacaagct
     1021 tcgaagaaaa agaagattcc aggtttgcac cctacgctgt caaacactca ttgagcgggg
     1081 gccgtatttc cgaggaaaag ccgcaagaag agcgcacgtg cttccagaga gatcgcgatc
     1141 gcatcataca ttctaaagcg tttcgcaggc ttggctataa gactcaggta tttgtaaatt
     1201 cggaaggaga catgtttcgc acacgtctca ctcacagtct ggaagtggcc cagctttctc
     1261 gcagcgctgc ttcttacctc gggctcaacg cggattactg tgaaaccatt gcactggctc
     1321 acgacatggg ccattctcct ttcggtcacg cgggtcagga cattctgaat cgcttgatgg
     1381 ccaatcacgg tggattcgag cacaattgcc agactcttcg tattgtaagc gctcttgaga
     1441 cgcgcacgat tgactggaat ggtctgaatc tgacacggct ttctctgatt ggaatgatga
     1501 agcacgttcg catctatgag tgcgacacgg ctcttcgcga aattctagac tacagaagaa
     1561 acatttctcc ccctctggaa gctgtgctcg tggatacatg cgatcgactt gcttatcttc
     1621 atcacgattt tgaagacggc ctggattcgg gatttctaga tcgagcagag gtactatcgc
     1681 acccggcctt tcttcatgca cagcgtactc ttcaggatcg tcatgg
//
LOCUS       DAOQJL010000699         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_61577_length_1724_cov_3.152786, whole genome shotgun sequence.
ACCESSION   DAOQJL010000699 DAOQJL010000000
VERSION     DAOQJL010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61577_length_1724_cov_3.152786"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLG78_19815"
     CDS             complement(<1..1107)
                     /locus_tag="PLG78_19815"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE25426.1"
                     /translation="MALFILALAGAIIGIEIFVAISLRSQITSMMEADVKGHVTFAAS
                     YADFRFPGDYRIDPDTGALWKGKENLSTSDSPLVFEIKAYTSSDVTIFRGDTRVATTL
                     RDKSGKSLVGTKASAVVADTVLKQGKIFLGEADVVGEKFQSAYAPLRDRDGKIIGMLF
                     TGLHVDTYSAEMRKTRLAIIVAGLFIIVLFGALLFWIVTRTLRPLFNLIDWTKSVSQG
                     DLRAEFKDENKARDEVGELSWALREMITNLKQMIMLIQNTAGDTRQSAENLIAFSEQF
                     SAGAQDQAAAAEEASAAMEEMAASAESVSERVSDSVQSMEAINQSLLLLSKSNQEVRG
                     SMGELTGLSRSASEKARVGEEQIHHATDAMARIQD"
     gene            1226..>1724
                     /locus_tag="PLG78_19820"
     CDS             1226..>1724
                     /locus_tag="PLG78_19820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE25427.1"
                     /translation="MPLLHVEDLKVHFPTRRGLARAADGVSFDLEEGKTHALVGESGC
                     GKSVTALAIAGLIPRERASISATSLRFRETDLLSLDPRDYRGLRGREIAMIFQEPMTA
                     LNPVMKVGNQILEAIEIHEDSTPTEERKKRVVQLMASTGLAEPDVLYEKYPHQLSGGM
                     RQRIMI"
BASE COUNT          400 a          447 c          457 g          420 t
ORIGIN      
        1 gtcctggatt cgtgccattg catctgttgc atggtgaatt tgctcttcgc ctactcgtgc
       61 tttctcggag gcggatctgg acaatccagt caattcgccc atggatccgc gtacttcctg
      121 atttgatttg gaaagaagaa gcaggctttg attgatcgct tccatagact ggactgaatc
      181 agaaactcgc tcactcacgc tttccgcaga tgcagccatt tcttccatgg cggcggaagc
      241 ttcttccgcg gcggcagcct ggtcttgcgc gcccgcagag aactgttctg agaaggcgat
      301 caagttttcc gcagactgtc ttgtgtctcc ggctgtgttt tgaatcagca tgatcatctg
      361 cttcaagttc gtgatcattt ccctcagggc ccaggaaagt tcgccgactt catcgcgcgc
      421 cttgttttcg tctttgaatt ccgctcggag gtcgccctgc gaaacactct tggtccaatc
      481 gatcaggttg aatagcggac gaagcgtgcg agtaacgatc cagaatagga gcgcaccaaa
      541 gagaacaatg ataaagagac ccgctacaat gatcgcaagc cttgttttcc tcatctctgc
      601 agagtacgta tcaacatgga gcccggtgaa gagcatgcca atgatctttc catcacgatc
      661 gcgtaacggt gcgtaggcgg attggaattt ttcacctaca acgtctgctt caccaagaaa
      721 gatcttgccc tgctttaata ctgtgtctgc aaccacagcg gacgcttttg tgcccacgag
      781 gcttttcccg gatttatctc tgagtgtggt ggcaacgcgg gtatcgcctc ggaagatggt
      841 aacgtcagat gaagtatatg ccttgatttc gaaaacgaga ggagagtcgc tggtgctgag
      901 gttttctttt cccttccaga gtgctcccgt gtccgggtcg attctgtaat cccccgggaa
      961 ccggaaatcg gcataggaag cagcaaaggt gacatgaccc ttcacatctg cttccatcat
     1021 agaagtgatc tggcttctca gggaaatggc aacgaagatc tctatgccga tgatcgcgcc
     1081 tgcgagcgca aggatgaaca gcgccataaa gcggaatcgg aggccaaatc ggacgctgcc
     1141 cgtggtcata cattagattg tcggttgggt gtggcccgct cttaatttga attgcatgag
     1201 gaagcagtag aaacgctctg tgcctgtgcc tttgctccac gtagaagacc tgaaagttca
     1261 ttttcccacc agaagaggcc tggccagagc ggcggacggt gtctcttttg atctggaaga
     1321 aggaaaaacc cacgcacttg taggggaatc tggctgcggc aaatctgtta cagctcttgc
     1381 gattgcgggt ctcattccca gggagagagc aagcatctcc gcaaccagcc tgaggtttcg
     1441 ggaaacggac cttctttccc tcgatccacg cgactatcgt ggactgcgcg gccgtgagat
     1501 cgccatgatc tttcaggagc caatgactgc gctcaatcca gtcatgaaag taggcaacca
     1561 gatcctggag gcaattgaga ttcacgaaga ttctactccc acggaggagc gcaagaagcg
     1621 agtcgttcaa ctcatggcat ccaccgggct ggctgagcca gacgtgctct atgaaaaata
     1681 tccgcaccaa ctgtctggcg gcatgcggca aagaatcatg atcg
//
LOCUS       DAOQJL010000700         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_62097_length_1717_cov_3.336943, whole genome shotgun sequence.
ACCESSION   DAOQJL010000700 DAOQJL010000000
VERSION     DAOQJL010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62097_length_1717_cov_3.336943"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1053
                     /locus_tag="PLG78_19825"
     CDS             7..1053
                     /locus_tag="PLG78_19825"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNE25428.1"
                     /translation="MKKIGVMIVDDSAVVRTVLTTVLTSVSDIEVIATAGDPIFAMEK
                     MKKRWPDVIVLDIEMPRMNGIDFLKTLMRDHPTPCVICSSLTAEGTSVAMQAMAAGAV
                     SIISKPKMGTKEFLEESRDRIVDAVRGASQSQLKPIDAQDLPPRAPEPAADKPAVHTS
                     YKRMVVLGASTGGTMAIEQVLSGLPADCPAVLIVQHMPEKFTHAFADRLDKSMRMSVR
                     EAQNGDPIMPGRVLIAPGNQHMEIKRTATGLEAVVRSGDYVNRHRPSVDVLFESAAKL
                     GGTSFIGVILTGMGGDGSQGLLSLKDKGARTIAQDQATSVVFGMPQEAIKLGAAELVL
                     PIDKIAAEILKLAR"
     gene            1142..>1717
                     /locus_tag="PLG78_19830"
     CDS             1142..>1717
                     /locus_tag="PLG78_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE25429.1"
                     /translation="MIQNNYFNTNEDLKKQFSDIIDWKEIVDAFENGFADAEEYKKTS
                     NEHLQYAPSNYEDAIEYYRTLLENLGDITGTTVASLAAEMDRIGLKYDSGNVTFPDAM
                     IKAYKAVQETGLQPYGISRRRGGLGIPCTVQALMMELLGRADASLAIALGCANLAETI
                     ERFASKEMSDKYVPLMASGELTGAMALTEPNY"
BASE COUNT          466 a          417 c          456 g          378 t
ORIGIN      
        1 aaagcaatga agaaaatcgg cgtgatgatt gtggatgatt ctgccgttgt tcgcacggtc
       61 cttaccactg tactcacttc tgtctctgac attgaagtca tcgcaactgc aggtgatcca
      121 atctttgcga tggagaagat gaagaaaaga tggccggatg tcattgttct cgacattgaa
      181 atgccgcgca tgaacggaat tgatttttta aaaactctga tgcgcgatca tccaactccg
      241 tgtgtcattt gctcttctct gactgcggaa ggaacttctg tcgccatgca ggcgatggcc
      301 gcaggtgcgg tttccattat ttcaaaaccg aagatgggaa caaaggaatt cctcgaagaa
      361 agcagagatc gcattgtgga tgcagtccgt ggtgcctcgc aatctcagct caagccaatc
      421 gatgcacagg accttcctcc cagagcaccg gaaccagcgg cagataagcc ggcggtacac
      481 acaagctaca aaagaatggt tgtgctgggc gcatccaccg gtggaaccat ggccatcgaa
      541 caggtgttaa gcggactgcc agctgattgt ccggcggttc taatcgtgca gcacatgcca
      601 gaaaaattta cacatgcttt cgcggatcgc ctggacaaga gcatgcgaat gtctgtgcgc
      661 gaagctcaaa atggagatcc aatcatgcct ggcagggtgc tgattgctcc gggaaaccag
      721 catatggaaa tcaaaagaac tgccacgggt ctggaagcag tcgttcgcag cggggactac
      781 gtgaatcgcc acaggccaag cgtggatgtt ttgtttgaat ccgcggcgaa gctgggaggg
      841 accagtttca tcggtgtcat tcttacaggt atgggtggag acggttcgca aggactgcta
      901 tcgctcaaag acaagggcgc gcgtacgatt gcccaggatc aggccacatc cgttgtgttc
      961 gggatgcccc aggaagcaat caaactggga gccgcggaac tcgtgcttcc cattgacaag
     1021 atagccgcag aaatccttaa actcgcgcga taagcccggg cgtgatgcca ttaatcgcaa
     1081 ttgaccgggt atctgccggt ttttttctcg actgacacgg tgttattccg gggagcattg
     1141 catgattcag aacaattact tcaatacaaa tgaagaccta aagaagcaat tcagcgatat
     1201 cattgattgg aaagaaatcg tagatgcatt tgagaacggt tttgcagacg cagaggaata
     1261 caaaaagaca agcaacgaac atctgcagta cgcaccctcc aactacgaag acgcaatcga
     1321 atactacaga acccttctag agaatctggg cgacattaca ggaacgactg tcgcttcgtt
     1381 ggcagctgaa atggaccgaa tcggtctgaa atatgacagc ggcaacgtca cgttcccgga
     1441 tgcaatgatc aaagcataca aagccgttca ggaaacaggc ctgcagcctt acggaatcag
     1501 ccgtcgtcgc ggtggcctgg gaattccttg cacggttcaa gctttgatga tggaacttct
     1561 aggaagggca gacgcatctc tggctattgc tcttggttgt gcgaatctcg cagaaacaat
     1621 cgaacgattc gcctccaaag agatgtccga caagtatgtg ccgctgatgg catccggtga
     1681 actcacaggt gccatggcgc tcacagaacc aaactac
//
LOCUS       DAOQJL010000701         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_63589_length_1697_cov_3.720463, whole genome shotgun sequence.
ACCESSION   DAOQJL010000701 DAOQJL010000000
VERSION     DAOQJL010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63589_length_1697_cov_3.720463"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1281)
                     /locus_tag="PLG78_19835"
     CDS             complement(4..1281)
                     /locus_tag="PLG78_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25430.1"
                     /translation="MRAPLHSVFPVSFGSISFLLFAITGCGGGSKETRVAYTETKSSA
                     EIVMQEHRRFTDRGWNTIIADSVIVHRFLNPVEFPAACDPAEKKPELSLDPDAERVAY
                     RCNVSAPWHIYFLQKSGSKAFELCATPAHLDWASVPSFEKARVSFLECESIQNNIFAN
                     TFKEIVAESRSRGGSASVADFLRDTISTSFASVFDPSGDDWDRAFRDLPASERAKLQP
                     FLKKTLEAPYNPYAVHRVVASMELSDDLDDLMVRAGEQMIASRPSGTEDPLITGMRAQ
                     TFDVVVRQLGRTNPAAAARIACKDAEAFSRNGISIVIPRAQLAAIARQKIPCDIGGVL
                     DRSKCDGGFACCYRGFTCGDSVRHACTAAELSELVKMELAQDPKKPWPSGGGFGYELL
                     VAVAMNQNSESARQFLERVKKVPVCDAPDSKKP"
     gene            1364..>1697
                     /locus_tag="PLG78_19840"
     CDS             1364..>1697
                     /locus_tag="PLG78_19840"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE25431.1"
                     /translation="MAHIQTNNRYPGILELLWYKPSTGQKLSALAHELLLGPSTLSSG
                     ERETIAAYTSALNDCEFCCESHNASACAHLGKDRPASVYLTPEFLAEQTPKMKALLEI
                     AALTQKGGR"
BASE COUNT          437 a          471 c          407 g          382 t
ORIGIN      
        1 tgatcaaggc ttttttgaat ctggcgcgtc gcaaacgggg accttcttca cccgctcgag
       61 gaattgcctg gcagattcac tattctgatt catagccact gcaaccagga gttcataccc
      121 gaacccacca ccggatggcc agggtttttt agggtcctgg gccagttcca tcttcacaag
      181 ttcggatagt tccgcagccg tgcatgcgtg cctcaccgaa tcgccgcagg taaaccccct
      241 gtagcaacaa gcgaaaccgc cgtcgcattt gcttctatcc aatacgcccc cgatgtcgca
      301 tggaattttt tgacgcgcta tggccgccag ttgcgcgcga ggaatcacaa tggagattcc
      361 atttcgagaa aaagcttccg cgtccttaca ggcaattctt gctgccgccg ctggattcgt
      421 ccgtccgagc tgacggacta ccacgtcaaa cgtctgcgca cgcattcctg tgatcaaagg
      481 atcctctgtg cctgaaggtc tgcttgcgat catctgctct cccgcacgaa ccatcaaatc
      541 atccaggtcg tctgaaagct ccatagatgc gacaacacga tgaaccgcat aaggattgta
      601 aggtgcttca agagtcttct ttagaaaagg ctgcaatttc gcgcgttcgc ttgctggtaa
      661 atcccggaat gcccgatccc agtcatcgcc agatggatca aacacacttg cgaacgaggt
      721 cgagatcgta tcgcgcagaa aatctgccac actggcgcta ccgccgcgag aacgcgattc
      781 agccacgatt tctttgaagg tattagcaaa gatgttgttc tggatggact cgcattccag
      841 aaaagacacg cgcgcctttt cgaaagatgg aacagaagcc cagtccaggt gagcgggcgt
      901 tgcgcacagc tcaaatgctt tgcttccaga tttctgcaaa aagtaaatat gccagggcgc
      961 agatacgttg caccgatacg cgactcgttc agcatctgga tccaatgaaa gctccggctt
     1021 tttctcagcg ggatcgcagg cagcaggaaa ttcgaccggg ttaaggaaac ggtgcacaat
     1081 cactgaatca gcaatgatcg tgttccatcc gcgatccgtg aatcttctat gctcttgcat
     1141 gacaatttct gcggatgatt ttgtttctgt atacgcaacc ctggtttcct tactcccacc
     1201 gccgcagcct gtgattgcaa ataacaggaa ggagatggaa ccaaacgaaa cgggaaaaac
     1261 ggaatgaagc ggcgctctca tcgcgcaaag tagaaggcac ggaaacagat gggtcaagat
     1321 aatttctgac gacaaatgcg gactctattt gaatctccca accatggcgc atattcaaac
     1381 aaacaacaga tatcctggta tcctcgaatt gctctggtac aagccatcca ccggacagaa
     1441 gctttctgcg ctggcgcacg aactcctgct tggtccatcc acgctctcct ccggcgaacg
     1501 cgaaacaatt gctgcataca cgtctgccct taacgattgc gaattctgct gcgaatcgca
     1561 caacgcaagc gcctgtgctc atctggggaa agacagacca gcatcggtgt atcttacccc
     1621 ggagtttcta gcggaacaaa ctcctaaaat gaaagcactc cttgaaatcg cagcgcttac
     1681 acagaagggc ggaagaa
//
LOCUS       DAOQJL010000702         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_63669_length_1696_cov_3.665448, whole genome shotgun sequence.
ACCESSION   DAOQJL010000702 DAOQJL010000000
VERSION     DAOQJL010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63669_length_1696_cov_3.665448"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLG78_19845"
     CDS             complement(<1..196)
                     /locus_tag="PLG78_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNE25432.1"
                     /translation="MNLEKWNLGVIENDELFREEVIRRLGDLPAARSVRAWPSAEQFL
                     RDPSHKGIEILFLDIMLSGIT"
     gene            415..>1696
                     /locus_tag="PLG78_19850"
     CDS             415..>1696
                     /locus_tag="PLG78_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HNE25433.1"
                     /translation="MLTFTVGLSLSVIGLLSLRRNENRPLLVTFSLFCFGIGILGLLL
                     AFRAIVLNLDILLLLNRALYFGVVCLSPGAYMFAYFLTAKRYKPLLVMGIFAAASAVF
                     GWFGLITGYDFTGSWFHYSFGNYPIAQLPLKLWGLIAALGYFVAWIPATVRYFRTHPQ
                     EAATKKALFFSMHMISLLVISNLPSLAGIPVFPMSTFAFLPMLVLGYSIFRGDFLNVN
                     DLLFRKRLLFKFLAGLLTFGFIGISVLVAVTLPPATHSAIYERPFFLLPLFSAVCAFA
                     LAIFLAGSNPDQKLNMLAAASLMLAGTFMLVMTSFKLNLPLIVTRRIEQILYTAFLFT
                     PGVHLRFVYLALGRKSPRAVRLVDLVSALLCVVLWTPYFFDGFHEYSFGRISAGGPGL
                     NAYGALGLFAAGAFLFSWWKVRKEAQSKLGSLVVL"
BASE COUNT          331 a          452 c          400 g          513 t
ORIGIN      
        1 cagtgatccc ggacagcatg atgtctagaa agaggatttc aatccctttg tgggaagggt
       61 cccgcagaaa ttgctccgca gaaggccacg cacgaacgga acgtgctgcg ggcagatctc
      121 caagccggcg aatcacttct tctcgaaaga gctcgtcgtt ttcgattact ccgaggttcc
      181 acttttccaa attcatgtcc atacacagta ctggtgtgac ctgacggaga tcaaacaaaa
      241 agaaaaccgc aacccccctg aacagggggt ttttgaataa aaaggatctg ctcttgcttg
      301 actgctgttt gtgaacgtta gacgccctac acgatgctac ccttgccgcc actgtctgat
      361 catcctgggg ccgtcgagta tttctggtac caattgccgt gggccgttcc tggtatgctt
      421 acgtttacgg tcggtttgtc tctctctgtg attggactcc tttccctgcg tcgcaacgag
      481 aatcgtccac ttctggtgac cttcagctta ttctgttttg gcatagggat tctaggattg
      541 cttctggctt ttcgcgccat cgttttgaat ctagacattc tccttctgct caatcgtgcc
      601 ctgtacttcg gcgtagtgtg cctttctccg ggtgcgtaca tgttcgcgta ctttctaacc
      661 gcaaagagat acaaaccact tcttgtcatg ggcattttcg ccgcggcatc tgctgtgttt
      721 ggctggttcg ggttgatcac aggatacgat tttaccgggt cctggtttca ttattcgttt
      781 gggaattatc ccatcgctca attgccgctg aagctgtggg gcctgattgc agcacttgga
      841 tatttcgtag cctggattcc agccacagtc cgctactttc gcacgcatcc acaggaggcc
      901 gcaaccaaga aggcactgtt cttcagtatg cacatgattt ctctgctcgt gatctcgaat
      961 ctgcccagtc ttgcgggcat tccagttttt cccatgagca catttgcatt tttgccaatg
     1021 ctcgttcttg gctatagcat ctttcgcggt gactttctca atgtgaacga tctactcttt
     1081 cgcaagagac tgctgttcaa atttctcgcg ggccttctca cgttcgggtt tattggaatc
     1141 tctgttctag ttgcagtcac tcttcctcct gcaacgcact cggccatata cgaacgccca
     1201 ttttttcttc tgccgctctt ctctgccgtt tgcgccttcg ctctggcgat ctttctggcg
     1261 ggaagcaatc cagatcagaa actcaacatg ctggccgcag cttctctcat gcttgcaggc
     1321 acattcatgc tcgtgatgac ttctttcaaa ctgaatctgc cattgatcgt aacacgacgc
     1381 attgaacaaa tcctatacac tgcatttctt tttacacccg gagtccatct tcgctttgtt
     1441 tatcttgcgc tcggaagaaa gagcccgcga gcggtgcgac tcgtggactt agtctctgcg
     1501 ctgctatgcg tcgtactctg gactccgtac ttcttcgatg gctttcatga atacagcttt
     1561 ggtcgcattt ctgccggagg tcctggacta aacgcgtatg gtgcactcgg attgtttgcc
     1621 gcaggtgcat ttctttttag ctggtggaaa gtgcggaaag aagcgcagag caaactcggc
     1681 agtctggttg ttttgt
//
LOCUS       DAOQJL010000703         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_63741_length_1695_cov_3.612195, whole genome shotgun sequence.
ACCESSION   DAOQJL010000703 DAOQJL010000000
VERSION     DAOQJL010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63741_length_1695_cov_3.612195"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1695)
                     /locus_tag="PLG78_19855"
     CDS             complement(115..>1695)
                     /locus_tag="PLG78_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNE25434.1"
                     /translation="EMVYSLRFENPANKSDFFVVESLVEAMALCAAHGPRLPKPERIK
                     RNVAGGEIRLNAQNEALQPSSGGLIEFWSPPVANEIRDDQGICMVNPDTGRFIHYYLA
                     GAYDSNIALIVSYGNGRRENLERLADILRRMELRGSDLSTNRDFHYGILNFCLGLHPM
                     LKPDTKFVLPYLASVGALAKEIEATDPEAAYASLRKRYSQLYGKPGDLAVSQKLTLVA
                     RPVEQLLSRPHLCMGWLVLNHRRSFKIENGKVVWLRNPMFVLRDLYHYLRLETRENAP
                     PSHVIWDHDLVLLNQACSFAAELEKAAGIDAEQAFQDSLARSEENSFTKFIDLDRDLR
                     AANGSSAFAQAINRRGAGLFEKACASYVGWTAGAELMSLVVQLGVRCGAFEFGVDAEL
                     YPITPEKFLSAETQKQLTRALAPPPKASADQIVAVTGGMFYAQESPSTPPFLTVGHHF
                     EKGDPIYIVEVMKMFNKIYAEFSGTVEEILVKGSGTVVRKGQPLFRVKPDEVIHLETD
                     AERDDRRRKHTDEILKSV"
BASE COUNT          413 a          455 c          426 g          401 t
ORIGIN      
        1 ggcagcatcg ggggctccca ccttcagcga ttcgccgtga ccctccccat ggtgatgaga
       61 atgatccggc gaatgcgtgt gatcgtgctc tgcgatgact atctccgaaa gacgttagac
      121 gctcttgagt atctcatctg tatgcttgcg cctacgatcg tcgcgctccg catctgtttc
      181 gagatgaatg acttcatccg gtttcacacg gaaaagtggc tgtccttttc gcacaaccgt
      241 tccagatcct ttcaccagga tttcttccac agtccctgag aattcagcgt agatcttatt
      301 gaacatcttc atgacttcaa caatgtagat cgggtctccc ttttcaaagt gatgaccgac
      361 cgtcaggaaa ggaggcgtgc tgggcgattc ctgagcatag aacataccgc cggttacagc
      421 tacaatttga tccgcggacg ctttcggtgg tggcgcgaga gcacgagtca gttgcttttg
      481 cgtctctgcg ctgaggaact tctcaggagt gatcggataa agctctgcgt ccactccaaa
      541 ctcaaacgcg ccacaacgaa cgcccagctg cacaaccaga ctcatcaatt ctgcgcctgc
      601 agtccaacct acgtaggacg cacatgcctt ttcaaagagg ccagcaccgc gacgattgat
      661 tgcctgcgcg aaagcagacg atccatttgc cgcgcgaagg tcccggtcca gatcaatgaa
      721 cttcgtaaat gaattctctt cggatcgcgc gagactatcc tggaaagcct gttctgcgtc
      781 gattcctgca gccttttcca gctctgccgc gaaagaacag gcctgattga gcagtaccag
      841 gtcgtgatcc cagattacat gcgatggagg agcgttctca cgagtttcga ggcgcaggta
      901 gtgatagagg tctctcaaaa cgaacatggg atttcgcaac cagacaacct ttccgttttc
      961 aattttgaaa gatctgcgat gattgagcac gagccaaccc atgcaaagat gcggacgaga
     1021 gagcaattgc tctacgggcc ttgcaaccag agtcagcttc tggctgaccg caagatctcc
     1081 aggcttccca tacaattgcg aataacgttt gcgaagagag gcgtacgcgg cctctggatc
     1141 ggttgcttca atttcttttg caagggcacc aacggatgca aggtacggaa gcacaaactt
     1201 cgtatccggc ttgagcattg gatgaagacc cagacagaaa ttcaagattc catagtgaaa
     1261 atcacggttc gtagacagat ctgatccgcg cagctccatg cggcgcaaaa tgtccgcgag
     1321 acgttccagg ttttcgcgac gaccgttgcc atagctcacg atcaaagcaa tgttggaatc
     1381 gtatgcacct gcgaggtagt aatggatgaa tcggccagtg tcaggattca ccatgcagat
     1441 gccctgatcg tctcgaatct cgtttgctac aggtggtgac cagaattcaa tgagaccgcc
     1501 cgaagacggc tgcaaagctt cgttctgtgc attgagacga atttcgccgc ctgccacatt
     1561 gcgcttgatt ctctcaggct ttggaagacg tggaccatgc gctgcgcaaa gtgccattgc
     1621 ttctaccaga gattctacca caaagaagtc actcttgttt gccggattct cgaaacgaag
     1681 tgaatagacc atctc
//
LOCUS       DAOQJL010000704         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_64127_length_1690_cov_4.324159, whole genome shotgun sequence.
ACCESSION   DAOQJL010000704 DAOQJL010000000
VERSION     DAOQJL010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64127_length_1690_cov_4.324159"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLG78_19860"
     CDS             <1..978
                     /locus_tag="PLG78_19860"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNE25435.1"
                     /translation="ISDACHLVLPLHRIIDAHRENAAGEKKIGTTKRGIGICYGDKTM
                     RIGLRAGDVLHADRLRDRLGHILEIKNYELKNLYRLPEVEFNALYEDLLAFGNEIRGM
                     VKNTSLLLNENLAKGKTVLLEGAQGTGLDIDFGTYPYVTSSNTTTGGAIAGSGIDFRF
                     LKTVTGISKAYVSRVGEGPFPTELFGAEVEKLRELGHEYGATTGRPRRCGWFDVELVR
                     HAARVNGLTEIALTKIDVLSAYDTIKIGVGYKRGGEKLPAFPTDGYLEGVEVVYEEMP
                     GWKKEIGHVLKYSDLPPECCKYIRRLEELCEVPIKLVSVGPGREHTIPL"
     gene            complement(980..1552)
                     /locus_tag="PLG78_19865"
     CDS             complement(980..1552)
                     /locus_tag="PLG78_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE25436.1"
                     /translation="MKGRGARKLELALEQIHLPIQDSYCIDLGASHGGFVRTLLENGA
                     ARVSAVDVAYGILDYSLRVDPRVSAIERSNVRNIEPSWFEDLPSSGKIIVTCDVSFMS
                     IRTVLASLADFLHGTGLAMEGLFLVKPQFEDSKSTVKGILKDPIRREEIISEIRSFCV
                     TKGFEVKGRMDLPEISGKNLEVFLHLSYEK"
BASE COUNT          428 a          403 c          437 g          422 t
ORIGIN      
        1 atttcagatg catgtcatct ggttcttcct cttcacagaa tcatagacgc tcaccgcgag
       61 aatgcggcag gtgaaaaaaa gatcggaacc acgaagcgtg gaattggaat ctgctacggt
      121 gacaagacca tgcgaattgg tctgcgcgcc ggcgatgtgc tgcatgcgga tcgcctgaga
      181 gacagactgg ggcacattct tgaaatcaaa aactatgaac taaagaatct ttatagattg
      241 cctgaagtgg aattcaacgc tctctatgaa gaccttctgg ctttcggaaa cgaaattcgc
      301 gggatggtaa agaacacttc gcttcttctc aatgagaatc tggcaaaagg taaaacagtg
      361 ttgctagaag gcgctcaggg aaccggactc gatatcgatt ttggaactta tccatacgta
      421 acctcgagca ataccacgac tggcggtgcg attgcaggca gtggcattga ttttcgtttt
      481 ctgaaaactg tgactggcat ttcaaaagct tacgtttcgc gcgtgggaga aggaccattt
      541 ccaaccgagt tgtttggcgc agaagtagaa aagctcagag agctcggtca tgagtacggg
      601 gccacaacag gtcgccctcg tcgttgcggc tggttcgatg tggaactcgt gcgccacgct
      661 gcgcgtgtga acggtctgac tgaaattgca ctcacgaaga tagatgtgct ctctgcgtac
      721 gataccatca agatcggtgt gggttacaag cgtggtggtg aaaagctccc tgcatttccg
      781 acggatggat acctggaggg agtggaagta gtctatgagg aaatgccggg ctggaaaaaa
      841 gaaatcggtc atgttttgaa atattcggac ctgccgcctg agtgttgcaa atacattcgc
      901 aggctagaag agctttgcga agtgcctatc aagctagttt cagttggacc aggccgcgaa
      961 cacacaatcc ctctttgatc tacttttcgt aggaaaggtg caggaacact tccaggtttt
     1021 tgcccgagat ttcgggcaaa tccattcttc ctttcacttc gaatcctttc gtcacgcaga
     1081 agcttctgat ttcagagatg atctcttcgc ggcgaatcgg atcttttaag attcctttta
     1141 cagtactctt agagtcttca aactgaggct ttaccaggaa caatccttcc atggcaagtc
     1201 cagttccatg cagaaagtcc gcgagagatg caagtaccgt tcgaatggac ataaacgaca
     1261 cgtcacacgt aactatgatc tttccagaag aaggaagatc ttcgaaccag gatggttcta
     1321 tgtttctcac gttggatctt tcgatggcac tcacgcgcgg atccacacga agactgtaat
     1381 cgagaattcc gtaagccacg tccacggcgc tcaccctggc ggcaccgttt tcaagcagcg
     1441 ttcgcacaaa tccaccatgg gacgcaccca gatcaatgca gtatgaatcc tggataggta
     1501 gatgaatttg ttccagagcg agctctagct tgcgcgctcc ccgacctttc atctggttct
     1561 gtctttgagt gaccgggtca attctgcgag cgcatgacgt ctggtttcgc tgatcggggc
     1621 ccgggccagg taagaaaggg cgcgtgtttc caggtccgcg caaattcgca tggactcttc
     1681 cattccgtat
//
LOCUS       DAOQJL010000705         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_64221_length_1689_cov_3.154223, whole genome shotgun sequence.
ACCESSION   DAOQJL010000705 DAOQJL010000000
VERSION     DAOQJL010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64221_length_1689_cov_3.154223"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="uppS"
                     /locus_tag="PLG78_19870"
     CDS             complement(<1..615)
                     /gene="uppS"
                     /locus_tag="PLG78_19870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNE25437.1"
                     /translation="MSQENTNPRHVAIILDGNGRWAKKRGLSRSEGHRAGGEALDRLL
                     DDALEIGIPFISLYAFSTENWKRPRAEIQAIWKLMREFFTDRLQRCIEKGIRVHASGD
                     LSRLPAANRKLILHVIEKTAKNKALTANFCVNYGSMDEISHAATEIVRERLSLAKKGK
                     KADAAVSAKEIEKHLYTHPIPPVDLLVRPGGESRLSNFLLWQCAY"
     gene            complement(617..1183)
                     /gene="frr"
                     /locus_tag="PLG78_19875"
     CDS             complement(617..1183)
                     /gene="frr"
                     /locus_tag="PLG78_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNE25438.1"
                     /translation="MPDKPVDSAAVLAELKAKMQKSLDSLVKDLAGIRSTRANAAMLD
                     GVHVEYYGSSVPLNQVASLSVPEARMLVITPFDKSALGEIEKAILKSDVGITPSNDGS
                     LIRLILPELSMERRQELVKQVKVRLEECRVSIRNLRRDGNEEIKKIAGKSQDETKSLQ
                     DDVQKLTDSFIHKAEDAAAQKEKGILTV"
     gene            complement(1176..>1689)
                     /locus_tag="PLG78_19880"
     CDS             complement(1176..>1689)
                     /locus_tag="PLG78_19880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNE25439.1"
                     /translation="MGMLATVLNALSLQDACEKVGVITRLQSAIEMKSICEGYIRRKA
                     MRHLEKNRIVIFAGGTGNPYFTTDTTAALRAVEVGCQVILKATKVDGVYDSDPKKNPD
                     AKRFSRVSYMESIQRRLKIMDSTALALCMDNNTPILVFDIFEPDSLKNLVDGKKIGTL
                     ISSEEGLEYA"
BASE COUNT          367 a          428 c          422 g          472 t
ORIGIN      
        1 gtacgcacat tgccatagta agaaattgct gaggcgactt tctcctccgg gtcgaaccag
       61 caagtcaaca ggcgggatcg gatgagtgta tagatgtttt tcgatttctt tcgcgctcac
      121 ggctgcgtcc gccttcttcc ctttcttcgc aagagacaac ctttcgcgca cgatctcagt
      181 ggcggcgtga ctgatctcat ccatggagcc atagttcacg caaaagttgg ccgtcagtgc
      241 tttgttcttc gccgtctttt cgatcacatg cagaatcaat tttctatttg cggccggtaa
      301 cctggacagg tctccggaag catgcacgcg aattcctttt tcaatgcacc tttgcagacg
      361 atcggtgaag aattcgcgca tcaacttcca gattgcctgg atttccgctc gaggtcgctt
      421 ccagttttca gtactgaatg catagagtga tatgaatgga atgccaattt caagagcatc
      481 atcgagcaaa cggtctagag cttctcctcc ggcgcggtgg ccttcggagc gcgacagacc
      541 gcgcttcttt gcccagcggc cgtttccatc gagtatgatg gctacatgcc gcggattggt
      601 gttttcctgg gacacgttag accgtcagaa tgcccttctc tttttgagcc gcggcgtctt
      661 ctgctttgtg aatgaaacta tctgttagct tttgcacgtc atcttgaagg gatttcgttt
      721 catcctggga cttgcctgca atcttcttga tctcttcgtt tccgtcacga cgaagattgc
      781 gaatggaaac gcgacattct tccagacgaa ccttgacctg cttgaccagt tcctggcgtc
      841 tctccataga aagttcagga agaatgaggc gaatcagaga gccgtcgttg gaaggagtga
      901 ttcccacatc tgatttcaaa attgcttttt caatttcacc cagggcactt ttgtcaaatg
      961 gagtgattac gagcatccgc gcttctggaa cagaaaggct ggctacctgg ttgagcggaa
     1021 ctgaggagcc gtagtattcc acgtgcaccc cgtcgagcat ggccgcgttt gcgcgcgtag
     1081 aacgaatccc ggctagatct tttacaagag agtccaggct cttttgcatt ttggctttca
     1141 gttctgcaag aactgcggcg gaatcaactg gtttgtcagg catactcgag gccctcctcg
     1201 gaagatataa gtgttccgat cttcttgccg tctacgagat tcttcaagct gtccggttca
     1261 aaaatatcga aaaccagaat gggggtgttg ttgtccatac agagagccag agcggtggaa
     1321 tccatgatct tgagtctgcg ctggatggat tccatgtagg aaacgcgcga aaaacgtttt
     1381 gcgtcaggat tcttctttgg atcggaatca tatacgccat ctactttcgt tgcttttaga
     1441 atcacctgac agcctacttc gaccgcccgc agtgcggctg tcgtgtcggt tgtaaaatag
     1501 ggattccctg ttccgcccgc gaaaatcaca atccgattct tttcgagatg cctcattgcc
     1561 tttctgcgaa tgtatccttc gcagatggat ttcatttcaa tggctgattg aaggcgagtg
     1621 atgacgccca ccttttcgca tgcgtcctgc aaagatagag cgttgagcac ggtggccagc
     1681 attcccatg
//
LOCUS       DAOQJL010000706         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_64375_length_1687_cov_3.506740, whole genome shotgun sequence.
ACCESSION   DAOQJL010000706 DAOQJL010000000
VERSION     DAOQJL010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64375_length_1687_cov_3.506740"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLG78_19885"
     CDS             <1..191
                     /locus_tag="PLG78_19885"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE25440.1"
                     /translation="IEEFAALTGTGALLNTSLNLHGLPLVNDHRQAFHVLDNSGLDFL
                     LIEDVLLVRKKTGETQRT"
     gene            219..1133
                     /locus_tag="PLG78_19890"
     CDS             219..1133
                     /locus_tag="PLG78_19890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HNE25441.1"
                     /translation="MGTAATAAIATLKKNGFAKLSQKVLSSEETKEFESIIDRLHADK
                     SISVDYSANSPSAASLIGRDQRLDFLLNKILAHPEVQGVLSQVVGDDFKIWEISTRIS
                     MDTDPGLGMHVDAVGQCNLAYLINDQTTPAGVTSFWPGSHLLPRSFINKVGLALDWIG
                     SYYWKKMFVPLTGTAGDVAFFVNKTYHGRLPAPASGGQRKIILMGCFPVGSRFKPIQQ
                     HVPFDSIEQPELRKRLSLSQGVHTMGDGFVMVGPEGAKSSSYTMSIEPAGPASFKTFW
                     MASKARLANLCFGVYKRLRPNRSEVSHA"
     gene            1144..>1687
                     /locus_tag="PLG78_19895"
     CDS             1144..>1687
                     /locus_tag="PLG78_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNE25442.1"
                     /translation="MERKDTIKMYKLPVEVKFCRKCTVSNQRPRITFDENGVCSACNF
                     AEYKKTKIDWAQREKELKELCDRFRRNDGGYDVIVPGSGGKDGGFVAHQLKYKYGMNP
                     LTVTWAPLKATEIGRKNLDAFIASGFDNILGTPNGKTTRRLTELAFRYLGDPFQPFIY
                     GQTNFPMHMAVNHGIQLIMYG"
BASE COUNT          426 a          408 c          447 g          406 t
ORIGIN      
        1 ttatagagga atttgccgcg ctcaccggaa ccggagcact tctgaatacg agtctcaatc
       61 tccacggtct gcctcttgtg aatgatcaca ggcaggcatt tcatgtgctt gataattcgg
      121 gtcttgattt tcttttgatt gaagacgttc tacttgtgcg aaagaaaact ggtgaaacgc
      181 agcgtactta aaacgacacg ctgagtctgg aggaatttat gggaacagca gcaaccgccg
      241 caatcgcaac gctgaagaaa aatgggtttg cgaaattgtc gcagaaggtg ctctcgagtg
      301 aggaaacaaa agagtttgaa tccatcattg atcgcttgca cgcggacaaa tcgatcagcg
      361 ttgactattc tgcaaatagt ccgagcgccg catcgcttat tggtcgcgac caacgtctgg
      421 atttcttgct gaacaaaatt ctggcgcacc ctgaagtgca gggagtgctt tctcaagttg
      481 tcggtgatga tttcaagatc tgggaaattt caacaaggat ctccatggac acagatcccg
      541 gactgggaat gcacgtggat gcagtcgggc agtgtaacct ggcgtatttg atcaatgatc
      601 agaccacacc ggcgggagtg acttcttttt ggccaggatc gcatctcctt ccgcggtctt
      661 tcatcaacaa ggtgggtctg gcgctggatt ggatcggctc ttactattgg aagaagatgt
      721 tcgtcccact cacgggtact gccggtgatg tggcgttctt tgtgaacaag acgtaccacg
      781 gtcgtcttcc tgcccccgcg tcaggtggtc aaagaaagat cattctcatg ggatgctttc
      841 ctgtcggctc gcgcttcaag cctatccagc agcacgttcc gtttgattct attgagcaac
      901 cagagttgcg caagcgcctt tctctttcgc agggtgtcca tacaatgggt gacggatttg
      961 ttatggttgg tccagagggc gcgaaaagct cctcctacac gatgagcatt gaaccagcag
     1021 gccctgcaag cttcaaaaca ttctggatgg cctccaaagc gcgcctggca aacctgtgct
     1081 ttggcgttta caaaaggctg cgccccaata gatcagaagt cagtcacgcc tgacaggaaa
     1141 aaaatggaaa gaaaagatac gatcaagatg tataaacttc cggtggaagt caaattctgc
     1201 cggaaatgta ccgtatccaa tcagcgcccg cggatcacgt tcgacgaaaa cggcgtctgc
     1261 tccgcgtgca actttgccga atacaaaaag acaaagattg attgggcgca gcgcgaaaaa
     1321 gaactgaagg aactgtgcga ccgctttcgt cgcaacgacg gtggatacga tgtgatcgtg
     1381 cctggcagtg ggggcaaaga cggcggtttc gttgctcatc agctcaaata caaatatgga
     1441 atgaatccgc ttaccgtgac gtgggcgcct ctgaaggcca ctgaaatcgg gcgcaagaat
     1501 ctggacgcat tcattgcatc cggtttcgac aatatactgg gcactccgaa tgggaagacg
     1561 actcgtcgcc tgactgagct ggcgtttaga tatctcggtg atccattcca gccctttatc
     1621 tatggccaga cgaacttccc gatgcacatg gctgtgaatc atggcattca gctgatcatg
     1681 tacggag
//
LOCUS       DAOQJL010000707         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_64671_length_1683_cov_5.342138, whole genome shotgun sequence.
ACCESSION   DAOQJL010000707 DAOQJL010000000
VERSION     DAOQJL010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64671_length_1683_cov_5.342138"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLG78_19900"
     CDS             complement(<1..961)
                     /locus_tag="PLG78_19900"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNE25443.1"
                     /translation="MITQERKPEIEKSIETSLMIAAPSWPLQNTVAVNPFWFLKDKLF
                     RDVVVNLAPVVNSPLLMPMEFYLQRYEEGTVTAKALEDAMRDARPHSPDLPHSLDSFL
                     MQSKDAHTRLRALPTFAEFLDTDGYWQRTVTNDLGKFAAGYFDDDQAIFPFPWKSLSF
                     WQAWCEARAYDQAMEICGASGFNASVAPLKSMSCSEAVIYMIREMDFKTDEARTLYMQ
                     RLIASILGWATRFKYVEWQTMLGYDQIRSSTAMELLCVRLAYDFGLFHTTRKSSSPEW
                     REEYEKIIETDFASQLTLVWQAALERSYQDRVAGVLQRPSPQDH"
     gene            complement(1003..>1683)
                     /locus_tag="PLG78_19905"
     CDS             complement(1003..>1683)
                     /locus_tag="PLG78_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE25444.1"
                     /translation="VMATQNDIKKKLAYSTISQMGMMMFGCGLGAFSIALFHIFAHSF
                     YKAHAFLSTGNLTAESKKRGLKLQAPSMFILLLTSMIGLAIIAIGVNYHSGDYLAYFV
                     YGAVLQLGLFQNIGHADLSSSSRIRVTAITAIGLVLAVTAYAILEHAIGFTVLGIVGP
                     ATDGTSSAFIPIAAYLLFVASLFLCAQLMRPGQSDWEKRLYVYFWNGGYFSHWSTRWL
                     ASPDLQNR"
BASE COUNT          437 a          412 c          434 g          400 t
ORIGIN      
        1 aatggtcttg cggcgaagga cgctggagta ctccggccac gcggtcctgg taacttcgct
       61 caagggccgc ctgccaaacg agcgtgagct gagaggcaaa gtcggtctct atgattttct
      121 catactcctc tctccactca ggggaggaac tcttgcgagt cgtatggaat agaccaaagt
      181 catatgcgag gcgaacgcaa agtagttcca ttgcagtgga agatcgaatc tgatcatacc
      241 cgagcatcgt ctgccactcg acgtatttga aacgggtcgc ccatccaagg atgctcgcaa
      301 tgagcctttg catgtaaaga gtgcgcgctt catcagtctt gaaatccatt tcgcggatca
      361 tgtagattac agcctcggag caactcatgg atttcaatgg agccacggat gcgttgaagc
      421 ctgaagcacc gcaaatctcc atagcttgat cataagctcg cgcctcgcac caggcctgcc
      481 agaaagataa agatttccag ggaaagggaa agattgcctg atcgtcgtca aagtagcctg
      541 cggcgaattt tccaagatcg tttgtaactg tgcgttgcca gtatccatcc gtgtctaaaa
      601 attccgcgaa agtcggcaac gctctgagtc gcgtgtgcgc gtctttcgac tgcatgagaa
      661 aagaatccag cgaatgtggt aagtcgggtg aatgtggtcg cgcatctcgc attgcatctt
      721 cgagcgcttt tgcagtgaca gtaccctctt cataacgctg caggtaaaat tccatgggca
      781 tcaggagcgg cgaatttaca acaggtgcaa gattcactac aacgtcgcgg aagagcttgt
      841 ccttgagaaa ccagaacgga ttgaccgcca ctgtattttg caagggccat gagggggccg
      901 cgatcatcag gctcgtttca atggactttt cgatttctgg ttttctttct tgcgtgatca
      961 tactcttctc ctggttcgaa ttctcttgga tcaggaatga acttatctgt tctgcaagtc
     1021 aggcgaagcg agccatcgcg tggaccagtg tgaaaaatat ccgccattcc agaagtatac
     1081 gtagagtctt ttctcccagt cactttgtcc cggtcgcatg agttgagcgc ataagaacaa
     1141 actcgcgaca aagagaagat aggcggcgat cggaatgaac gccgacgatg tcccatcagt
     1201 agccgggcca acgatcccta acacagtgaa gccgatggcg tgttcgagga tggcgtaggc
     1261 tgtgactgcc agtacaagac cgattgctgt gatggccgtc acccgaatac gcgaagaaga
     1321 acttaaatct gcgtgtccaa tattctgaaa aagcccgagt tgcaagacag cgccgtagac
     1381 aaaatacgcc aggtaatcac cggaatgata gttcaccccg attgctatga ttgcaaggcc
     1441 aatcatactc gtgaggagga gtatgaacat agacggagcc tgtaatttca aaccacgttt
     1501 ctttgattct gcggtcaaat tgcccgtgga aagaaatgcg tgcgctttgt agaagctatg
     1561 agcgaagata tggaacaaag cgatgctgaa tgcaccaagc ccgcagccga acatcatcat
     1621 ccccatctgg ctgatcgttg agtaagcaag cttcttcttg atatcatttt gtgtggccat
     1681 cac
//
LOCUS       DAOQJL010000708         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_65542_length_1672_cov_5.259122, whole genome shotgun sequence.
ACCESSION   DAOQJL010000708 DAOQJL010000000
VERSION     DAOQJL010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65542_length_1672_cov_5.259122"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLG78_19910"
     CDS             <1..1076
                     /locus_tag="PLG78_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25445.1"
                     /translation="MMDSYPMAQASVDVSSGKPVFVRAEGKVAVKRTSGDWTQDFARE
                     LILPFSFPGAFWRVTVHDLERAVFQIAITFHHSVADGRSGFSFMLDFLRRLDQYPAQL
                     ETERREFPSDRFAPFLRNAKSPRLTTDSMPWFAKRSQPSSPQMDCFTLEKEETSRLVS
                     RARENKCTVHGWIGAAQILSLSSLFGDDRSHTLALSTPADVRSRLYGGAPDAGLELCI
                     SLVTTAIAATEMGTVARELSQHIRSELASDAFLQFYEALPDPVTLLSNPKGLTLFATM
                     MQRGVQASVLSNVGIVSPPVFQKIAVESMSFTVHPMITQPVFVTVTTFEGRMHFVLNH
                     DSNRWESRTFDSFLESFRNSLAG"
     gene            complement(1080..1517)
                     /locus_tag="PLG78_19915"
     CDS             complement(1080..1517)
                     /locus_tag="PLG78_19915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE25446.1"
                     /translation="MPKPVPFAFSIPMKVAWGDMDAFGHVNNVSYARYFETARAEFFS
                     SFRLWAAKLDKQSSGPVLIHMDLDYRKQTVYPETLSITVGITSASRRSFSMGCSMWNE
                     KQECVLTATADFMWFDFDAGKPSTIPEQFHKLALAEAERRKER"
BASE COUNT          375 a          412 c          441 g          444 t
ORIGIN      
        1 agatgatgga ttcttatcca atggctcagg cgagcgttga tgtttcttct ggcaaacctg
       61 tctttgttcg cgcagaggga aaggttgctg tgaagcgcac cagtggcgat tggacacaag
      121 acttcgcgcg tgagttgatt cttccattct cgtttccagg tgcattctgg cgagtgaccg
      181 ttcacgatct ggaaagggca gtctttcaga tcgccatcac gtttcatcat agcgtggcag
      241 atggaaggtc cgggttttca ttcatgctgg atttcttgcg tcgcctggac cagtatccgg
      301 cgcaattaga aacagagagg agagaatttc catcggatcg ttttgctcct tttcttcgca
      361 atgcgaagag ccctcgtttg acaacagact ccatgccctg gtttgcaaag agatcgcagc
      421 cttcttctcc gcagatggat tgcttcaccc tggagaagga ggagacttcc aggctcgtgt
      481 cgcgggcgcg cgagaacaaa tgcacggtgc acggctggat tggtgcggca caaattcttt
      541 ctctttctag cttatttgga gatgatcgca gtcacaccct ggctctttca actccggcag
      601 acgttcgctc gaggctctac ggcggtgccc cagatgccgg gttggagctg tgcattagcc
      661 tggtcacgac tgcaatcgcg gccacagaaa tgggaacggt tgcgcgagaa ctttctcagc
      721 acataagaag tgaactggcc tcagatgcgt ttctgcaatt ctacgaggca cttcctgatc
      781 cggtcactct cctttctaat ccaaagggcc ttacactgtt tgcaacgatg atgcagcgcg
      841 gtgttcaggc aagcgtactg agcaacgttg gaattgtgag tcctcccgtg ttccagaaga
      901 tagcagtgga atccatgagc tttactgtgc atcctatgat cacgcaacca gtctttgtca
      961 cagtcaccac gtttgagggc cggatgcatt ttgttttgaa tcatgactcg aatcgctggg
     1021 agagccgcac gtttgattct tttctagagt cttttcgcaa ttccctggca ggatagcgcc
     1081 taacgttcct ttcttctttc cgcttccgcc agagcgagtt tgtggaattg ttccgggatc
     1141 gtagatggtt ttccggcatc aaaatcaaac cacatgaaat ctgcggttgc agtcagtaca
     1201 cattcctgct tttcattcca catagaacat cccatggaaa aagatctacg ggaagcactt
     1261 gtgatgccca ctgtgatgct gagtgtttcg ggatagacag tttgtttgcg atagtccagg
     1321 tccatatgga tgagaacagg tccggaggac tgcttgtcta gcttcgcggc ccacagacga
     1381 aagctggaaa agaactctgc gcgagccgtt tcaaaatacc gcgcgtagct tacgttgttt
     1441 acgtggccga atgcgtccat atcgccccag gcgactttca tcgggatgct gaaggcaaat
     1501 ggaaccggct ttggcatctc gcagggttct aagtgcgaag catccatcaa gcattattca
     1561 cggaagccct cggagcggaa acttagagtg ggatgcaaat gaattgcgca acagggccgt
     1621 cgtaaaatgt ttcgatcata tcatgcgaaa tctgatttcc cgtttttgtg ac
//
LOCUS       DAOQJL010000709         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_65556_length_1672_cov_3.658009, whole genome shotgun sequence.
ACCESSION   DAOQJL010000709 DAOQJL010000000
VERSION     DAOQJL010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65556_length_1672_cov_3.658009"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1354
                     /locus_tag="PLG78_19920"
     CDS             134..1354
                     /locus_tag="PLG78_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE25447.1"
                     /translation="MERIFAQPMSFADIKEKSDRYLVNNVKRAPVAFYFGQGEYLYDT
                     ENKRYLDFLSGVAVTCLGHGDADFIEAMREQSDRVVHTSNLVYNQELALFAEALVSYS
                     FPGRVFVCNSGTEANEAAFKVARLHGQKKGNANLIVTLEGSFHGRTVAGMCMTAQAKI
                     HQGFGPLLDHFHYIRPNDIEMLEKEMDQHGSEMCALVLELVQGEGGVRPLDAEYVKAA
                     RQLCDENDVLLILDEIQTGMGRTGKLFAYEHFDIVPDAMTLAKGLGNGFPIGAVVIRE
                     DRADLIHPGVHGTTFGGNHLGARVAYETLKIIVTRDILDNVNALSDYFLRRLKGMQLN
                     LKCITDVRGMGLHIGVELDRPGAAVVDRCRELGLLVNCTAETTIRIVPPLNLPLESAA
                     EGLDILEKVFQELK"
     gene            1351..>1672
                     /locus_tag="PLG78_19925"
     CDS             1351..>1672
                     /locus_tag="PLG78_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNE25448.1"
                     /translation="MKSLFAFIFCLGAFPLHAIVPVPPLQGRVQDLTETLTAEEKTIL
                     EQKLADFEKTKGSQVVVLIVPSTEEEAIEQFSIRVAEAWKIGRKGVDDGVILLVAKSD
                     KKLRI"
BASE COUNT          420 a          372 c          481 g          399 t
ORIGIN      
        1 cggactggac ttgcgggata agacggaaca atcgaggttg agaatggact ggaaagtaga
       61 agaaacggaa ggacagcgga ctgctagact gagtgccgcg gcggaaggaa gagacctggc
      121 accgggagaa agaatggagc gcattttcgc gcaacccatg tctttcgctg acattaagga
      181 aaaatcggat cgatatcttg taaacaatgt caaacgagcg cctgtcgctt tctactttgg
      241 ccagggcgaa tatctgtatg atacagagaa caagcgatac ctggattttc tatctggagt
      301 ggcagtgacc tgtctgggtc atggagatgc agatttcatt gaggcgatgc gcgagcagtc
      361 tgatcgcgtt gtgcacacgt ccaatctggt gtacaatcaa gaacttgcgc ttttcgcaga
      421 agctctggtc agctattcct ttccaggtcg cgtatttgta tgcaattccg gcacagaagc
      481 aaatgaggcc gcgttcaaag tggcgcgctt gcacgggcag aagaaaggca atgcaaatct
      541 aattgtaact ctggagggaa gttttcacgg aaggactgtg gctggaatgt gcatgaccgc
      601 acaggcaaag atccatcagg gctttgggcc tctcctcgat cactttcact acattcgtcc
      661 caacgacatc gaaatgctgg aaaaggaaat ggaccagcat ggatccgaaa tgtgcgcgct
      721 ggtgctcgag ctggtccagg gtgaaggcgg tgttcgtcca ctggacgcgg aatacgtgaa
      781 ggctgcgcgt cagctctgcg acgaaaacga cgttttgttg attctggatg aaattcagac
      841 tggcatgggc cgtaccggga agcttttcgc gtacgaacac ttcgatattg ttccggatgc
      901 tatgactctt gcgaagggcc ttggcaatgg attcccgata ggcgccgttg tgatccgcga
      961 agatcgcgcg gacctcattc atcccggggt tcacggaact acatttggtg ggaatcacct
     1021 gggcgctcgc gttgcgtatg aaacgctcaa gatcattgtt acccgcgata tcctggacaa
     1081 tgtaaatgca ctgtcagatt attttctgcg gcgactgaaa ggaatgcagt tgaatttgaa
     1141 atgcattacg gatgtgcgcg ggatgggctt gcacattgga gttgagctcg acagacctgg
     1201 agccgctgta gtggatcgtt gtcgtgaact tgggcttctt gtgaattgca ctgcggaaac
     1261 cacaattcga atcgttccgc ctctgaacct tccgctggaa tcagccgctg aaggactgga
     1321 catactggag aaggtgtttc aggaactgaa gtgaagtcgc tgtttgcgtt tatcttttgt
     1381 ctgggtgcct ttccgctcca tgcaattgtg cctgtacctc ctctgcaggg cagagttcag
     1441 gatcttacag aaacgctcac tgcagaagag aagaccattc tcgaacagaa actcgctgac
     1501 ttcgaaaaaa caaaaggctc gcaggttgta gttctgatcg taccttcaac ggaagaggag
     1561 gcaatcgaac aattttcgat tcgcgtcgcg gaagcctgga agatcggaag aaagggtgta
     1621 gacgatggcg tgattcttct cgttgcaaag tcggacaaaa agttgcgaat tg
//
LOCUS       DAOQJL010000710         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_66717_length_1657_cov_4.053683, whole genome shotgun sequence.
ACCESSION   DAOQJL010000710 DAOQJL010000000
VERSION     DAOQJL010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66717_length_1657_cov_4.053683"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLG78_19930"
     CDS             complement(<1..188)
                     /locus_tag="PLG78_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE25449.1"
                     /translation="MIEIVAKAPDRLAMRITDDLKMENAREFFDLFREHYTDSQVQVL
                     IDFGKIRFVDSSGIGILLR"
     gene            complement(185..382)
                     /locus_tag="PLG78_19935"
     CDS             complement(185..382)
                     /locus_tag="PLG78_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25450.1"
                     /translation="MADVAVGGQYKLNRRTFLHKGIFVNQGVSVQVMEITDGPDKVIV
                     QFLDREGFPHILTGIGPEELE"
     gene            complement(375..1160)
                     /locus_tag="PLG78_19940"
     CDS             complement(375..1160)
                     /locus_tag="PLG78_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE25451.1"
                     /translation="MDFERFKKINDERMNYREMEDASVVSSYRNLGCGDGYRLYLKVE
                     GDKVVDASYTTTGCGFGLVSLAMATQWITGKTIEQAGSIQASDIEGMFEFPDRRKNYP
                     ESAVEAVQKAVADFKNGTGIRPEDRVSRTTALEALARDGHLRGAKLNQIILEGENLEG
                     VDASGADFSNAFLQNVNFRGANLSGARFRGAFLNNANLEGANLSGADLRWAKLTGARL
                     EGVQVQGATYDIGTRVDANRIHLFKEMVQAGKDAYVEKEAGVG"
     gene            complement(1169..>1657)
                     /locus_tag="PLG78_19945"
     CDS             complement(1169..>1657)
                     /locus_tag="PLG78_19945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNE25452.1"
                     /translation="PVIADGGISTIGDLANALAIGASTTMMGSMFAGTQEAPGDYFYE
                     NGVRLKKYRGMASLEAMEAGGDKRYFSEDQEVKVAQGVAGFVVDKGSMMQFVPYLVQG
                     LRQSFQDMGARSISELHEALYSGKLRFEKRSYSAQLQGSVHSLHSFTGPALGGWEQNN
                     RR"
BASE COUNT          392 a          473 c          371 g          421 t
ORIGIN      
        1 cgcagaagaa tgccaatgcc ggaactgtcc acaaagcgaa ttttgccaaa gtcgattaga
       61 acctgcacct ggctgtccgt ataatgctcg cggaacaggt caaagaactc gcgggcattc
      121 tccatcttga gatcatccgt gattctcatc gcaagccgat ccggcgcctt tgcaacaatc
      181 tctatcattc cagctcttcc ggaccaatgc ccgtcagtat atggggaaat ccctcacggt
      241 ccaaaaactg cacaataact ttatcaggtc cgtccgtaat ttccatcacc tggacggaca
      301 ctccctggtt tacgaaaatg cccttatgaa gaaaagtgcg gcgattcagc ttatactggc
      361 cacccactgc aacgtcagcc aactccggcc tctttctcta cgtaggcgtc tttgcctgcc
      421 tgaaccattt ctttgaacag gtggattctg tttgcatcca ctcgcgtacc aatatcgtaa
      481 gttgcgccct gaacttgaac gccttcaagg cgcgctcccg tgagctttgc ccagcgaagg
      541 tccgctccgc tgagattggc gccctctaga ttcgcattgt tcaaaaaagc tccgcgaaac
      601 cgcgcgccag ataagttcgc tccgcgaaag tttacattct gtaagaacgc atttgaaaag
      661 tccgcaccgg acgcatccac accttccagg ttttcgcctt ccagtataat ctgattgagc
      721 ttcgcaccgc gaaggtgccc atcgcgtgcc agcgcctcaa gagcagtcgt gcgagagact
      781 ctatcttccg gacgaatgcc tgtgccattc ttgaaatcgg ccactgcctt ttgcactgct
      841 tccactgcgg attctggata attcttgcga cgatctggaa actcgaacat tccttctatg
      901 tcggaagcct gaatggaacc agcctgctct atggttttcc ctgtgatcca ttgagtagcc
      961 atggccagag atacaagacc aaagccgcaa ccagttgttg tgtaagaagc atctacaact
     1021 ttgtcacctt ctactttcaa gtacagtcga tagccgtctc cacatcccag attgcgatag
     1081 gaagaaacga cagacgcgtc ttccatctcg cgatagttca tgcgctcgtc gtttatcttt
     1141 ttgaatcgtt cgaaatccat gggaccgcct atcttcgatt gttctgctcc cagccaccca
     1201 gagctggacc ggtgaaacta tgaagactgt gcacagagcc ctgcaattga gcgctgtaag
     1261 atcttttctc aaatctcagt ttgcctgagt aaagtgcttc atgaagttca gaaatggaac
     1321 gcgcacccat gtcctggaag gactgtctga gcccctgaac caggtaggga acaaattgca
     1381 tcatagaccc tttgtctacg acaaatcctg ccacaccctg tgcgactttc acttcttgat
     1441 cttctgaaaa ataacgctta tccccgcctg cttccattgc ctcaagagaa gccatgcctc
     1501 tgtacttttt gagacgtaca ccgttttcgt agaagtaatc gccgggggct tcctgggttc
     1561 ctgcaaacat agagcccatc atggttgtgg atgcgccaat ggcgagtgcg ttggcaaggt
     1621 ctccaatggt tgagatgccg ccgtctgcta tgaccgg
//
LOCUS       DAOQJL010000711         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_67684_length_1645_cov_3.481761, whole genome shotgun sequence.
ACCESSION   DAOQJL010000711 DAOQJL010000000
VERSION     DAOQJL010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67684_length_1645_cov_3.481761"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLG78_19950"
     CDS             complement(<1..271)
                     /locus_tag="PLG78_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25453.1"
                     /translation="MNADIEQYLKGQADMIATYVQKLGKDDPAVTACLAVLEKLRTLG
                     GRYAAIYEFMADPSFQPLSAEFSAKMMELATKASRPAPGAAPSIPS"
     gene            complement(268..870)
                     /locus_tag="PLG78_19955"
     CDS             complement(268..870)
                     /locus_tag="PLG78_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25454.1"
                     /translation="MSSQDLAFARQYLEGRLREGQHRPDLHQTINSVYSRYLKILSDT
                     PDQYASSVGDMFDVWKSETIDRQLNLRKLAEKIGHAEAAAVHAKMAERVKATRSYPEL
                     ASLSAEVQEAARKAGASEIARRDALTAFLGFLVLIRAESEKNKPGRRAQARSAFQEMR
                     SLDPDFSFSKLRSHLPYRAMIYYTEEQLSMLEQTFAECKA"
     gene            complement(874..1383)
                     /locus_tag="PLG78_19960"
     CDS             complement(874..1383)
                     /locus_tag="PLG78_19960"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNE25455.1"
                     /translation="MQQDPTVISQNPYEAPAVASLPVEEPYSSGTLADPGMRLIAHII
                     DMIVFLGAFGVAMAGSSFLPQEKMALGIFGGMFGALVIIAINLYLLAARGQSIGKVAL
                     GIRIVRVSGEKASFARIFFLRIGVPAIINVVPGLGSMFALVDSLMIFQDSRQCIHDMI
                     ADTKVVRIR"
BASE COUNT          397 a          450 c          395 g          403 t
ORIGIN      
        1 cagacggaat cgacggagcg gcccccggcg caggacgaga agcttttgta gccagctcca
       61 tcatcttcgc tgagaattca gcggaaagcg gctgaaagga aggatctgcc ataaactcgt
      121 agatggccgc ataacgtcca cccagggtcc ttagtttttc tagcactgcc aggcatgcgg
      181 tcacagccgg gtcatctttg cccagcttct ggacgtaggt ggcgatcatg tccgcctgac
      241 ccttcaggta ttgctctata tctgcattca cgccttgcac tccgcaaaag tttgctccag
      301 catggacagt tgttcttctg tgtagtagat catggcacga tatggtaagt gagatcgcaa
      361 ttttgaaaaa ctgaaatccg gatcgagcga tctcatttcc tgaaacgcag atcttgcctg
      421 tgcccggcgg ccaggtttat ttttctcgga ttccgcgcga atcaaaacaa gaaagcccag
      481 gaatgcggtc agtgcgtccc tccgcgcgat ttcagatgcg ccggccttgc gcgcggcttc
      541 ctgcacttct gcagacaggg atgccagctc cggataggaa cgagttgctt tcacacgttc
      601 tgccatcttc gcgtggaccg ctgcagcctc tgcgtgccct atcttttctg ccagtttgcg
      661 aagattgagc tgtctatcga tggtctccga tttccacacg tcgaacatat ctccgactga
      721 agaggcgtac tggtccggtg tatcacttaa tatttttaag tatctactgt aaacagagtt
      781 gatcgtttga tgaagatccg gtctatgctg gccttcgcgc aaacggcctt cgagatactg
      841 acgtgcgaac gcgagatcct gagagctcat gaatcacctg attcgaacaa ccttcgtatc
      901 tgcaatcata tcatgaatgc actgcctgga atcctgaaaa atcatcaaag aatccacgag
      961 cgcaaacata gaaccgagtc caggaaccac gttaataatc gcggggacgc ctatgcggag
     1021 aaaaaagatc cgcgcgaagc tggccttttc cccgctcact cgaacaatgc gaatcccaag
     1081 tgcaaccttg ccgatggact gtccccgcgc cgccagtaag tacaaattga tcgcgatgat
     1141 gacgagagcc ccgaacatac cgccaaagat tccaagcgcc attttctctt gtggaagaaa
     1201 agaggaccct gccatggcca caccgaaagc cccgagaaac acaatcatgt cgatgatgtg
     1261 cgcaatgagg cgcattccag gatctgcaag tgtcccggag gaatatggct cctccacggg
     1321 aagcgacgct actgcaggtg cctcatatgg attttgactg atgacggtag ggtcctgttg
     1381 catgggtgaa gttcgaatgg ccaatctttg ctgtcaagga atgagcagcc atgattattg
     1441 attgaaatat actgctttcc tccgtctatt tgcatggtga ccgtctacga gcttacctac
     1501 attctcgttt cggccatcct ggtttatcgc gccattcaca atctggtcat cttctctaaa
     1561 aaccgcagac ttgatttcct cctattcttc gcactcactc aagccgcatt cacagcgtac
     1621 ctgatctgcc ttctcaaaac aatca
//
LOCUS       DAOQJL010000712         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_67897_length_1642_cov_4.696282, whole genome shotgun sequence.
ACCESSION   DAOQJL010000712 DAOQJL010000000
VERSION     DAOQJL010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67897_length_1642_cov_4.696282"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1449)
                     /locus_tag="PLG78_19965"
     CDS             complement(178..1449)
                     /locus_tag="PLG78_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE25456.1"
                     /translation="MSRRLGEILRKILKGPPLKFNPFVMSRVFYLWLFLGVAAGASAG
                     IYWIVLEQIIYFASAVEDWKVIPLMGGAGLLAGLIIHWIGDPGEIQLIVNNIRFRGGR
                     LDTKHNVSMILSSLLCVGTGGSLGPEAPLVQVTGSVGTWISERLRLKGEEFRSMTIAG
                     MAAGFTALFGAPLGGSLFALEILHHRHVVEYYEAIIPAIVSSLAAYTVFALLIHLGFG
                     PVWNVPAYTTQSVLDFGLAVVFGTVAAAVGWGFISVTNHLRVFVREISAPIFVKTCAG
                     GFLLGAISFYMPLSRFFGHREIELLFQGNFTIGFLIALLMAKLGAIAITVTTGWRGGF
                     IIPLFFMGAVLGLLLKQIFPSISLPLAAVCCMASLNSCVTRTPLSTTLLLSTMTGFSH
                     FVPILFASMTGYFLAPKTPFIITQQRPAGET"
     gene            complement(1454..>1642)
                     /locus_tag="PLG78_19970"
     CDS             complement(1454..>1642)
                     /locus_tag="PLG78_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25457.1"
                     /translation="HCAPGFDVCCDHDDPGQKNKYCKNITSGDLLNLSLPHSCQALDA
                     HQVHIREYGSLRRTLVSR"
BASE COUNT          459 a          424 c          426 g          333 t
ORIGIN      
        1 ttctgtacac atgctttcca agcatgcacc atcgaccact cggacacctc tcctggtccg
       61 gaccagaccg ccagagaggg gctcatggtc aaggacttca cggggcaatg cttgactgac
      121 gagcgcacct ggaagatcgc acgtgacaag gcgcatgtca gattctaatc tcgagtccta
      181 cgtctcaccg gccggccttt gctgagtgat gataaatggt gtttttggtg ccaggaagta
      241 gcctgtcata ctggcgaaca gaataggaac aaaatgagaa aagccagtca tcgtggagag
      301 cagaagggtc gtgctcagcg gagtgcgcgt aacgcaagag ttgagcgaag ccatgcagca
      361 aacggccgcc aggggcaggc tgatgctcgg aaaaatctgt ttcagaagta agccaaggac
      421 tgcgcccata aagaaaagag ggatgatgaa tccgccgcgc catcctgtcg tcacagtgat
      481 ggcaatggcg cctaactttg ccattaggag ggcgattaga aacccgattg tgaaatttcc
      541 ctgaaaaaga agttcgatct cacggtgacc aaagaagcgc gaaagaggca tgtagaaaga
      601 gatagcaccc agaaggaaac cgccggcaca tgtcttcacg aaaattggcg ctgagatctc
      661 tctaacgaac acgcgaagat gattcgtcac tgagatgaag ccccagccaa ccgccgcggc
      721 aacggttcca aacacaacgg caagtccaaa gtcaagaaca gactgtgtgg tatacgcggg
      781 aacattccag acaggaccaa accccagatg gatcaagagc gcaaacacgg tgtaggctgc
      841 aagactggaa acaatggcag gtatgatcgc ttcgtaatat tccaccacat gccgatgatg
      901 cagaatttcc agagcgaata gacttccgcc aagtggtgcg ccaaatagtg cggtgaatcc
      961 tgctgccatt ccggcgatgg tcatcgagcg aaactcttct cctttcaatc taagtctttc
     1021 ggaaatccag gtgcccactg aaccagtcac ctgcacgaga ggtgcttctg gtccaaggct
     1081 ccctcctgtg cccacacaca ggagagaaga tagaatcatc gaaacattgt gcttcgtgtc
     1141 cagtctccca ccgcgaaagc gaatgttgtt tacgatgagt tgaatttccc ccgggtcgcc
     1201 gatccagtgg atgatcagac cggccagaag ccccgcacct cccatcaagg gaatcacttt
     1261 ccagtcttcg actgccgatg cgaaatagat gatctgttct agtacgatcc aatagattcc
     1321 agcactcgct cccgctgcca ctccaagaaa caaccagaga tagaagactc tactcataac
     1381 aaacggattg aatttaagag gaggcccttt cagaatctta cgaaggattt ctcccaatcg
     1441 gcgtgacatt cagtcagcgc gaaacaagcg ttctgcggag cgagccatat tcccggatgt
     1501 ggacttgatg agcgtcaaga gcctggcagg agtgcggcag agagagattc agtaaatcgc
     1561 ctgacgttat gttcttgcaa tatttgtttt tttgtccagg atcatcatga tcgcagcaga
     1621 catcaaagcc gggagcgcaa tg
//
LOCUS       DAOQJL010000713         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_68293_length_1637_cov_4.006953, whole genome shotgun sequence.
ACCESSION   DAOQJL010000713 DAOQJL010000000
VERSION     DAOQJL010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68293_length_1637_cov_4.006953"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLG78_19975"
     CDS             complement(<1..528)
                     /locus_tag="PLG78_19975"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE25458.1"
                     /translation="MIQKSNYNSSPGDRKFDELLYERDRLYRILEVNTVISRSLRLDA
                     VLTLLMEKAKEVTESEASSLMRVDEEKQELYFHTIQGDKSDAIRHIRLKIGEGIAGWV
                     AKEGKPLLVADASKDPRFSRRADEASKFVTRSMMCVPLVVSRGVIGTVQVLNKKNGEF
                     FNETDLRLFEVMANQA"
     gene            548..799
                     /locus_tag="PLG78_19980"
     CDS             548..799
                     /locus_tag="PLG78_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25459.1"
                     /translation="MDQGSSPEDIFKARNRVNNDPGARSAPGPGLSGPYSNHDMQIRN
                     HLSGLVRAMLQDNKKAELREVLHAHRAFIDEILVLLEKQ"
     gene            complement(809..>1637)
                     /locus_tag="PLG78_19985"
     CDS             complement(809..>1637)
                     /locus_tag="PLG78_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25460.1"
                     /translation="QGRLVLHAPGRIAPDVYAKGKLRDTMYPIVPGAEYLFHSPEKPV
                     GGMDPILHALEGTSQKELFNQIVVSVLAGGQKNEDTQSEITKALSAWAKMPVPQDLFE
                     RQNAHEALRYLEWLHTARLGLDASAVKVVRSILAKHSMSRITNSQWMGMRGVRFNLVF
                     CGGKLTLQLCERVKMDPIRIRLPRTIEEIAADPKSYGAYLRQQTRALDKSDEPPGLRQ
                     VLEFAEKVYEDRLHVVDDRKRLHLLKENLGIKPQVSKKPVSMLDRILSRLGLKKLAA"
BASE COUNT          376 a          437 c          398 g          426 t
ORIGIN      
        1 cgcctggttg gccatgactt cgaagagtct aagatctgtt tcgttgaaga actctccgtt
       61 tttcttattg agcacctgta ctgttccaat cactccgcgc gaaacaacga gcggaacgca
      121 catcatcgag cgagttacaa acttactggc ctcatctgcc cggcgcgaaa atcgcggatc
      181 cttggaagcg tccgcgacta gaagcggttt gccttccttt gcgacccagc ctgcgattcc
      241 ttccccgatt ttaagacgta tgtgacgaat ggcgtccgat ttatcgccct gaattgtatg
      301 aaaataaagt tcctgcttct cttcgtctac gcgcatgagg ctagaagctt cggattcagt
      361 tacttccttt gccttctcca tcagaagagt cagcacagca tccaaacgca ggcttcttga
      421 aataaccgtg tttacttcaa gaatgcgata cagtctgtcc ctttcgtaaa gaagctcgtc
      481 gaactttcta tcaccgggag aggaattata gtttgatttc tgaatcatga actctcaggt
      541 agtacggatg gatcaaggct cctctccgga agatatattc aaggctcgta acagggtcaa
      601 caatgatcct ggggcgcgtt cggcaccggg accgggcttg tctggcccgt attcaaacca
      661 cgacatgcaa attcgcaatc atctctcggg gctggtcaga gccatgctgc aagacaataa
      721 gaaggcagag ctgcgcgaag tgctccacgc ccacagagcg ttcatagacg aaatcctggt
      781 tttgctggaa aagcagtgac ccgcagcctc aggctgcgag tttcttgagt cccagtctcg
      841 aaagaatccg atcgagcatg gaaaccggct tcttgctcac ctgaggcttg atacccaggt
      901 tttctttcag caggtgcaat ctcttgcggt catccaccac atgaagtctg tcttcgtaga
      961 ccttttccgc gaattcaaga acctgcctga gcccgggtgg ctcatcgctt ttgtccaatg
     1021 ctctggtctg ctggcgaaga taagctccgt atgactttgg atctgctgcg atttcttcaa
     1081 tggtgcgagg aagtcgaatg cgaattggat ccatcttcac tctttcgcac agctgcagag
     1141 tgagctttcc accacagaac accaggttga aacgaacccc gcgcattccc atccactggg
     1201 agttggtgat gcgcgacatg ctatgctttg ctagaatgga acgaaccacc ttcaccgcag
     1261 atgcgtccag accaagcctc gcggtgtgaa gccattccag gtatcgcagt gcttcgtgtg
     1321 cattctgtct ttcgaacaga tcctgaggaa caggcatctt tgcccaggcg ctcagtgctt
     1381 ttgtgatttc tgattgcgta tcttcattct tctgtccgcc cgctagaaca ctgacgacga
     1441 tttgattgaa gagttctttt tgcgaagtgc cttcgagagc atggagaatt ggatccattc
     1501 cgcccaccgg tttctccgga gagtgaaaca agtattccgc acctggaaca attggataca
     1561 tggtatcgcg taacttgccc tttgcgtaga cgtcaggcgc tattcttccg ggtgcatgaa
     1621 gcaccagacg accctga
//
LOCUS       DAOQJL010000714         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_68607_length_1633_cov_3.585551, whole genome shotgun sequence.
ACCESSION   DAOQJL010000714 DAOQJL010000000
VERSION     DAOQJL010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68607_length_1633_cov_3.585551"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="PLG78_19990"
     CDS             <1..109
                     /locus_tag="PLG78_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNE25461.1"
                     /translation="VVIVTALDNLVKGAAGQAVQNLNLMFGLPEGTGLA"
     gene            152..223
                     /locus_tag="PLG78_19995"
     tRNA            152..223
                     /locus_tag="PLG78_19995"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Pro,seq:ggg)
     gene            complement(287..>1633)
                     /locus_tag="PLG78_20000"
     CDS             complement(287..>1633)
                     /locus_tag="PLG78_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE25462.1"
                     /translation="MELLGRADASLAIALGCANLAETIERFASKEMSDKYVPLMASGE
                     LTGAMALTEPNYGSDLPGVQTKAEKGADGIWRLTGTKRFITHACGFAGTPSVILTLAR
                     TGSPTSGARGLSFFLVHGKDVHVASIEKKMGLHCSPTCEVVYDNSPGELIGEESQGLV
                     KYAMGMMNTARLSIAAQAMGIGSAAYFEAKKYASERVQFGKTIDKIPAVRKMLDLIER
                     ETAAMRCLLIEASRTVDMYLWRLERLEHSGLPDREVRKDEQVRKWEKLANYFTPLSKY
                     YTSEMCNKVAYDAVQIHGGSGYTEDYDVSRIFRDARITNIYEGTTQLQVVASIGGVVA
                     GMAPAGHLRAYLDEEMAKFQPSAELTQIRATLEDAITAYKSISDGDSRDMLSFELVEM
                     SARLIAGMLLERAASRQQGADRADRLRIAKLYNSDSQAICAANTTKIKNAGSLVTA"
BASE COUNT          338 a          420 c          441 g          434 t
ORIGIN      
        1 ggttgtgatt gtcactgctc tcgacaattt ggtaaaaggc gcagccggcc aggcagtgca
       61 gaatctgaat cttatgttcg gactccccga gggaaccggt cttgcctgaa gcgcttgaca
      121 ccaggccctc ttttcaaatg ctttgttgaa acgggatgta gcgcaggggt agcgcacacg
      181 tctggggggc gtgcggtcgc tggttcaaat ccagtcatcc cgatttgttt caaaaaaaag
      241 gccgggtttt cccggccttt ttgcgttcag atgctgggag ttcccatcac gctgtcacaa
      301 gagaacctgc gtttttgatt ttcgtggtgt ttgcagcaca gatagcctga ctgtctgaat
      361 tgtatagctt tgcaattcta agtctgtccg cacgatccgc gccctgctgg cgggatgcgg
      421 cgcgttccag aagcattcca gcaatgaggc gagcagacat ctctacgagt tcaaaagaga
      481 gcatgtcgcg cgaatcgccg tcgctgatgg atttgtaagc agtgattgcg tcttccagag
      541 tggcgcggat ctgagtgagc tccgcagacg gctggaattt tgccatttct tcgtccaggt
      601 aagcgcgcag gtgacctgcc ggagccatgc cagccacaac gccaccaatg gatgcaacaa
      661 cctggagctg tgtggttcct tcgtagatgt tggtgatgcg agcgtcgcgg aagattcgag
      721 atacatcgta gtcttctgtg taaccggacc cgccatgaat ctgaacggcg tcatacgcca
      781 ctttgttgca catttcgctg gtgtagtatt tggagagcgg tgtgaagtag ttggcgagtt
      841 tttcccactt gcgcacttgc tcgtccttgc gaacttcgcg gtctggaagg ccactgtgtt
      901 cgagtctttc cagtctccac agatacatgt caacggttcg tgaggcctca atgaggaggc
      961 atcgcatagc agctgtttcg cgctctatca gatccagcat ttttcgcaca gccgggatct
     1021 tgtcgatggt cttgccaaac tgcactctct ctgatgcata tttcttagct tcaaagtaag
     1081 ccgcacttcc aattcccatt gcctgggctg cgatagagag gcgcgcagtg ttcatcattc
     1141 ccatcgcata cttcacaaga ccctggcttt cttcgccaat tagttcaccg gggctgttgt
     1201 catagactac ttcgcaggta ggtgagcagt gaaggcccat tttcttttca atgcttgcga
     1261 cgtgaacatc tttgccgtgc acaaggaaaa acgaaagtcc gcgcgcgcca ctggtcggtg
     1321 atccggtgcg tgccagagtc agaattacgg aaggagttcc tgcaaatccg catgcatgcg
     1381 tgatgaatct ttttgttcca gtcaggcgcc atattccgtc cgcgcctttt tcggcttttg
     1441 tttgcacgcc agggagatct gaaccgtaat ttggttctgt gagcgccatg gcacctgtga
     1501 gttcaccgga tgccataagc ggcacatact tgtcagacat ttctttggac gcgaatcgtt
     1561 cgattgtttc tgcgagattc gcgcaaccaa gagcaatggc cagagatgca tctgcccttc
     1621 ctagaagttc cat
//
LOCUS       DAOQJL010000715         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_68685_length_1632_cov_4.379201, whole genome shotgun sequence.
ACCESSION   DAOQJL010000715 DAOQJL010000000
VERSION     DAOQJL010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68685_length_1632_cov_4.379201"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLG78_20005"
     CDS             complement(<1..870)
                     /locus_tag="PLG78_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25463.1"
                     /translation="MEWLLLLLAGGVAAGVGLWAVRPSPGEQGSSEGPSSSPGLPGVK
                     DDRGTAVPLGGKDTTDQIRDLLAKAPDKIQAKGVSRKQEPSRSSKVPLRVTYNTDQLI
                     ARSSRFAHHRRALQNAEILVSKQKATEALEVFERVANRVQDADIQSRIQTNIDDIRRW
                     LSGFESEDDQLKFPEIVIPLTLQNLALENLTEGIKNLSDSIVQKIAESVAAVSAISVQ
                     HTSPSGGYATSSPGSAPASPSASAPASSPASAPSAYAATPGAPAQTAMPGQAPGAAIP
                     TMGAAYAVPATADM"
     gene            938..>1632
                     /locus_tag="PLG78_20010"
     CDS             938..>1632
                     /locus_tag="PLG78_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25464.1"
                     /translation="MALPIQKINSRLEGEFVFRGRPYSVPFTLPGDLVQFKLSRKTGR
                     LGMEVLHIEKAHNTDFALADPFCAVYGRCGGCRGQHIPYPKQIELKTTDLVRAMEGLG
                     KKPEILVAPRLQGLRNRMDFVVDAGVTGFREAREFARFVDVDLCPAERDQSNRVLEVF
                     RSAIERHKDIAFVRESGEGIVKYATIRSGSTSLLSITIEASRSENQRYLAFLQDLIQT
                     LPPQCSLVETHTV"
BASE COUNT          354 a          416 c          470 g          392 t
ORIGIN      
        1 catgtccgca gtagcgggga ccgcataggc cgctcccatc gtagggatcg ctgcacctgg
       61 tgcttgcccc ggcatcgcag tttgagcggg cgcccctgga gtggctgcat aggcggaagg
      121 tgccgacgcg ggtgaggaag ctggcgcgga cgctgatggg gaagcggggg cagaacccgg
      181 agaagacgtc gcataaccac ccgaaggtga agtatgttga actgagatcg cggaaacggc
      241 agcaaccgac tctgcgattt tctgtacgat cgaatcagat agattcttga tgccctctgt
      301 tagattttcc agtgccaggt tctggagagt cagagggatt acgatttctg gaaatttgag
      361 ttggtcgtct tcgctttcaa atccggaaag ccatctgcga atatcgtcta tgttggtctg
      421 gatgcgcgac tgaatgtccg catcctgaac tcgattagcc actcgttcga atacttcgag
      481 cgcctctgtt gccttttgct ttgatacgag tatttcagca ttttgcagag ctcgtctgtg
      541 atgcgcaaat ctagaggagc gtgcgatgag ttgatctgtg ttgtatgtta cgcgaagagg
      601 gactttggac gagcgagatg gctcctgttt tctggagaca ccctttgcct ggattttgtc
      661 cggagctttt gcgagtagat cgcggatctg gtcggtggtg tccttgccgc cgagtggcac
      721 agcagtgcct cggtcatctt tgactccagg caaaccggga ctggaggatg gcccttccga
      781 gcttccctgt tcccccggcg agggacgaac ggcccacaga cccacacccg cggctacgcc
      841 gccggccaat agtaaaagta accactccat aaaaagcgtc ttgtcctgat tatcgacccc
      901 ggaagcttca gtatccaacg cctctggcga tcaactcatg gctttgccca ttcagaaaat
      961 caactccagg ctggagggcg aattcgtttt tcgcggaaga ccctacagcg ttccgtttac
     1021 tttgccgggg gatctggtgc agttcaagct atcccggaaa accgggcggc ttggaatgga
     1081 agtgcttcac attgagaagg cccacaacac agattttgcg ctggcggatc ctttttgtgc
     1141 agtgtatgga aggtgcggcg gttgcagggg ccagcacatc ccttatccaa agcagatcga
     1201 actaaaaacc acggacctgg tgcgagccat ggaagggctc ggtaagaagc ctgagatcct
     1261 ggttgctccc aggcttcagg ggcttcgcaa tcgaatggat ttcgtagtgg atgcaggagt
     1321 cacaggattc cgcgaggcgc gagagtttgc gcgttttgtg gatgtcgatt tatgtcccgc
     1381 agagagagat cagtccaatc gtgttctaga agtattccgg tctgcgatcg agcgccacaa
     1441 ggacatcgcg ttcgttcgcg aaagcggaga agggatagtg aaatacgcca ccattcgctc
     1501 cggttctact tcgcttcttt ctataacaat agaagcgagt cgctcagaaa atcaacgtta
     1561 tctggcattc ctgcaggatc tcatccagac cttgcctccg caatgctctc ttgtggaaac
     1621 tcacactgtt ta
//
LOCUS       DAOQJL010000716         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_68802_length_1631_cov_2.642132, whole genome shotgun sequence.
ACCESSION   DAOQJL010000716 DAOQJL010000000
VERSION     DAOQJL010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68802_length_1631_cov_2.642132"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLG78_20015"
     CDS             complement(<1..948)
                     /locus_tag="PLG78_20015"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE25465.1"
                     /translation="MDLSEAREVFMQECGALLDRMESLLIEESTNPRGERETIDSLFR
                     VVHTIKGSAGIFGFDRIERFAHDLESLMDRVRGGEKELNQGLVELLLRSRDHLSSLMQ
                     LLNVKEESAAAVMGANLDASQNSLLSEVRAMIDGGAIVPDAKPQAAASAVAKENTYRI
                     VLMFKPELFSHGLDPLPVVRNLRTCGEVLQFHLILDRLPALAAVSPETCYLGCEMILR
                     SSLSEKELRSELEFVQDDCEISISEESAPTSPAVTDQAAKAAPAPAPASAFAEPVSSS
                     SDPSYSKPSPAAAQPSESRFVRVESSRLDHLVNLVGELVI"
     gene            complement(939..1190)
                     /locus_tag="PLG78_20020"
     CDS             complement(939..1190)
                     /locus_tag="PLG78_20020"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE25466.1"
                     /translation="MRIRFPADLTISNAGPLKASLLALADRKENFQFDMQQVTEMDSS
                     CVQLILAFEREARARGCSIEYSLSEKARKISDIYGLPWT"
     gene            complement(1213..1578)
                     /locus_tag="PLG78_20025"
     CDS             complement(1213..1578)
                     /locus_tag="PLG78_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE25467.1"
                     /translation="MSKKVLIIDDSASIRKVVSLTLKGAGYEVDEAVDGQDGITKISA
                     SKYSLIICDVNMPNMDGLTFHRTMRQNESAKFTPVIMLTTESQEEKKWEGQEAGARAW
                     IVKPFKPEQLLGAVSKLIL"
BASE COUNT          372 a          433 c          420 g          406 t
ORIGIN      
        1 gatgacaagc tcgcctacca ggtttacgag atgatctagc ctgctggatt ccacgcgaac
       61 aaatctggat tcagatggct gcgcggccgc aggcgaaggt tttgaatagg atggatcaga
      121 tgaggaagag actggctcgg cgaatgcgga tgccggtgct ggtgctggtg ccgcttttgc
      181 cgcctggtcg gtcactgcgg gcgaagtggg agctgactcc tcagaaatag aaatctcgca
      241 atcgtcttga acaaactcaa gttcggaacg caattccttc tctgaaagag aggaccttag
      301 aatcatttcg caacccagat agcatgtctc aggcgataca gcggcgagag cgggaagtct
      361 gtccagtatg agatgaaact gaagcacctc tccacacgtt cgcagattgc gaaccacggg
      421 aagtggatcg agcccgtgcg aaaacagttc tggtttgaac atcaatacga ttcgatacgt
      481 attttctttg gctacggctg aagcggctgc ctgaggcttc gcgtctggaa caatggctcc
      541 cccgtcgatc atcgcgcgaa cttcggaaag caaagagttt tgagacgcat ctagatttgc
      601 acccatgact gcggccgcag attcttcctt cacattcaag agttgcatga gactcgaaag
      661 atgatctcgc gaacgaagaa gaagctcgac gagtccttga ttgagttcct tttccccacc
      721 ccgaacacga tccatcagtg attccaggtc gtgagcgaaa cgctcaatgc gatcgaaccc
      781 gaaaatgcct gcagaaccct tgatcgtgtg gactacgcga aaaagagaat cgatggtctc
      841 acgttcgccg cgtggattcg tggactcttc aatcagcagg ctctccatgc ggtcgagcag
      901 cgcgccacat tcctgcataa agacttctct ggcttcgctc aggtccatgg cagtccgtag
      961 atgtctgaga tttttctggc cttttctgac aatgagtact caatgctgca tccgcgggcg
     1021 cgcgcctctc tttcgaatgc aaggatcaat tgcacgcaag acgaatccat ttcagtgacc
     1081 tgctgcatat caaattgaaa gttctctttc cgatccgcca gtgcaagcag gcttgctttg
     1141 agaggccctg cattggagat cgtaaggtct gcgggaaaac ggattcgcac ctgtatcctc
     1201 cgccggccag ccttacagga tgagcttgga caccgcccca agcaattgct cgggcttaaa
     1261 tggtttcaca atccaggcgc gcgctcccgc ttcctgtcct tcccacttct tctcttcttg
     1321 agattctgtg gtgagcatga tgaccggagt gaacttcgca gattcattct gacgcattgt
     1381 gcgatggaat gtgagaccgt ccatgtttgg catgtttacg tcacatatga tcaggctata
     1441 ttttgaggct gagattttgg tgatcccatc ctggccgtct acggcctcat ccacttcgta
     1501 tcctgccccc ttcagagtca ggctcactac tttgcggatt gatgcggaat catctataat
     1561 caggactttc tttgacatgt acctctccgc ttttcgcgta ccctcaggga ctattgtcgg
     1621 cacgacggtg c
//
LOCUS       DAOQJL010000717         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_68860_length_1630_cov_3.057143, whole genome shotgun sequence.
ACCESSION   DAOQJL010000717 DAOQJL010000000
VERSION     DAOQJL010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68860_length_1630_cov_3.057143"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLG78_20030"
     CDS             <1..708
                     /locus_tag="PLG78_20030"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNE25468.1"
                     /translation="DTIERLHEFNPMLGHRGCRVAITYPEIYEMQVRAIFEAGCKLKQ
                     EGVDVVPEIMIPIVMNPNELTFIKNGKRIEGKYIKGIRDTAKEVFAETGVELKYHVGT
                     MVELPAAALLSDELARYAEFFSFGTNDLTQTTFGLSRDDITSFFPAYTEFDLLQNNPF
                     QVLGSPVKELIRISSDRGRLTRPDIKLGLCGEHGADPVNIEFCRDAGLNYVSCSSYSV
                     PIALLAVAQMNLKREAK"
     gene            complement(892..1587)
                     /locus_tag="PLG78_20035"
     CDS             complement(892..1587)
                     /locus_tag="PLG78_20035"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNE25469.1"
                     /translation="MSELILVRHGQSLWNLHNLFTGWVDIPLSDQGINEALKAGDRLA
                     NMNFDVVYVSTLVRAMQTALLALARNKSGRVPIVQPVKGHTDEEKKLAEWAKIYSDRA
                     IGNSIPVYKAWQLNERYYGELQGFNKQETADKYGEEQVHIWRRSYDVPPPNGEALKDT
                     AERTIPFFQNVIEPLLVMGKNVLISAHGNSLRSIVMHLDHLTREQVLALELKTGDPIL
                     YTMQSGKLVKGSV"
BASE COUNT          405 a          398 c          409 g          418 t
ORIGIN      
        1 gatacaatcg aacgtctgca tgagttcaat ccgatgctcg gccacagagg ttgccgcgtc
       61 gccattacat atcctgaaat ttacgagatg caggttcgcg ctatcttcga ggcgggttgc
      121 aaactcaagc aagaaggagt ggatgttgtt ccggaaatca tgatcccgat cgtgatgaat
      181 ccaaacgaac tcactttcat caaaaacggc aaaagaatcg aaggtaagta tatcaaaggc
      241 attcgagata ccgcaaaaga agtttttgcc gagaccggtg tggagctgaa ataccacgtt
      301 ggaaccatgg tggaacttcc cgccgccgct ctgctttcgg atgagctggc cagatacgcg
      361 gagttcttca gctttggaac caacgacctg acacagacca catttggact ttcaagagac
      421 gacatcacat cgttctttcc ggcatacacg gaatttgacc ttctgcagaa caatccattc
      481 caggtgctgg gaagtcctgt gaaagaattg attcgcatca gctcggaccg tggtcgtctg
      541 actcgtccgg atatcaaact gggactctgc ggggaacacg gcgcggatcc ggtgaacatt
      601 gagttctgtc gggacgccgg tttgaactat gtttcctgtt cttcctacag cgtgccgatc
      661 gcactgctgg ctgtagcgca gatgaacctg aaacgagaag cgaagtaatt cgagagttct
      721 gaacttccga tttacctgaa gccttcattc tccaatcggg gatgaaggct tttctctttt
      781 taaatgaatg actggattgc gagaggatcg ctctcgaatg cgataaataa agaaaaatca
      841 atcgggcggg aaagaaaaaa ccgcgccttt cgacgcggcc tgttttgaag cttagacgga
      901 acctttgacc agtttgccgc tttgcattgt gtagagaatg ggatctcctg ttttcaattc
      961 cagagccagc acctgttccc ttgtcagatg atccagatgc atgacgatgg agcgcagact
     1021 gtttccatgc gcgctgatta aaacattctt gcccatcaca agtagtggtt cgatgacatt
     1081 ctgaaagaaa ggtatggtcc gttccgcagt gtccttcaga gcttcaccgt ttggaggcgg
     1141 aacatcatag ctgcggcgcc agatgtgaac ctgctcttcg ccgtatttgt ccgcggtctc
     1201 ctgtttgtta aaaccctgaa gctctccata atatctttca tttaactgcc aggctttgta
     1261 gaccggaatg ctgttgccga tggcgcggtc agaatagatt tttgcccatt cagccagttt
     1321 cttttcttca tccgtatgtc ctttcacagg ctggacgatc ggaaccctgc cggacttatt
     1381 gcgtgccagg gccagaaggg ccgtctgcat ggctcgcacg agtgtggata cgtaaacaac
     1441 gtcaaaattc atgttagcaa gcctgtctcc ggctttcagc gcttcattga tgccctgatc
     1501 ggaaagagga atgtccaccc agcccgtaaa caggttgtgg aggttccata aagattggcc
     1561 gtgtcttaca aggattagtt cgctcatgga cccagtccta aattcagcca atctgatgtc
     1621 cagaggaata
//
LOCUS       DAOQJL010000718         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_71009_length_1605_cov_2.991613, whole genome shotgun sequence.
ACCESSION   DAOQJL010000718 DAOQJL010000000
VERSION     DAOQJL010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71009_length_1605_cov_2.991613"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PLG78_20040"
     CDS             complement(<1..1529)
                     /locus_tag="PLG78_20040"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNE25470.1"
                     /translation="MRALFAFLLFYSGFSLAAEPCVAPLKLSEEDPGAPLARRSRPLH
                     PGDPCETADSNIQKSLAEILKSNGGPVIRPSGSSLVKGMDLVSRRFALSSSDQKLLEK
                     NGFVVPERLDVPGYGFAFHEIYQSEMPVYVSVDAILHAIFASNDAIIKDVEKKTILPA
                     LIRILANLHCTLLRAGYDGETAMDLDVYLTVARSLIEDREVKSNTGVEREVSALVASA
                     TKASGLEEVALFGRKRIVDFSQYQPRGHYVDMPGFFRGVMWLSRLEFNLVSRSSRSSQ
                     PGDTPDPSETPREAKDAMALVDLIGRSGEMQSVIQLDHSWAVLAGKREDISPENLSTL
                     MRLKSISVGRAESFQKLKDAIGTGYARTIRMHYMPQGSDHLPVIFTLLGPRAVADAQS
                     LMPIVHPSVNSRTMISSVDAAYILGNDRAATYLTQDLLRFSTLRAKLDEARRNLNLDG
                     LDLYSLWMSAIRSLSATSPSSPSYMKSEAFADMRLNSTVAAFAQIKHNYVLLASQGYD
                     EG"
BASE COUNT          402 a          406 c          410 g          387 t
ORIGIN      
        1 ccctcatcgt atccctggga agcaagtagc acatagttgt gtttgatctg cgcaaaagcg
       61 gcgacagtgc tgttgagcct catgtctgca aacgcttcgc ttttcatgta agacggactt
      121 gacggggaag ttgcactgag acttcgaatg gcgctcatcc agagggaata gagatccagg
      181 ccgtccagat tcaggtttcg tcgcgcctca tccagttttg cgcgcaaagt agaaaatcga
      241 agaagatcct gcgtcagata cgtagccgcg cggtcatttc caagaatgta ggctgcgtct
      301 acagacgaaa tcatagtgcg actgtttaca ctcggatgaa caatgggcat cagactctgc
      361 gcatccgcca ctgcccttgg accgagaagt gtgaagatca cagggagatg atcagaaccc
      421 tgtggcatgt agtgcattcg tattgtgcga gcgtatcccg tcccaatggc gtcttttagt
      481 ttttgaaacg attccgcgcg gcccactgat atggatttga gtcgcatcaa agtggataga
      541 ttttccggag aaatatcttc acgcttacct gcgagaaccg cccagctatg atctagctga
      601 atgacgctct gcatttctcc cgagcggcca atcaaatcca cgagagccat tgcatccttt
      661 gcctcgcgcg gagtctctga tggatccggg gtgtcacccg gctgcgaact gcgggaagac
      721 cgcgatacca gattgaactc cagcctcgaa agccacatca ctccgcgaaa gaatcctggc
      781 atgtccacgt agtgtccccg cggttggtac tgactgaaat caacgattct ctttcttcca
      841 aacaatgcta cttcttccag tccggaagct tttgtggcac tggcaacgag tgcggagact
      901 tctctttcca ctccggtatt gctttttacc tcgcgatcct caatcaagct gcgtgcgaca
      961 gtgagataca cgtccaggtc catggcagtc tctccgtcgt aacctgcgcg aagaagagta
     1021 caatgcaaat ttgcaagaat gcgaatcaat gccgggagga tggtcttttt ctctacatct
     1081 ttgatgatgg catcattgga tgcaaagata gcatgtaaga tggcgtctac ggaaacatag
     1141 actggcattt cagactgata gatttcgtga aatgcaaagc cataacctgg aacatccagt
     1201 ctctctggaa ccacgaatcc atttttttca aggagcttct gatcagaact actgagcgcg
     1261 aatctgcgcg aaaccagatc catacctttc accaggctag aaccggaagg gcggatcact
     1321 ggcccaccgt tcgattttag aatttcagca agactcttct gaatgtttga gtccgcagtt
     1381 tcgcatggat cgccgggatg gagaggtctg gatcttcttg ccagtggagc gccgggatct
     1441 tcttccgaga gttttagggg agcgacgcag ggctcagcgg ccagagaaaa gccggagtag
     1501 aataacagga aggcaaacaa cgctctcatg gcccacattg cttttagatg accgggcgtt
     1561 gcaatcgttc tgaaaaaagg gcaataaaaa accgcggcac ttttg
//
LOCUS       DAOQJL010000719         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_72010_length_1593_cov_4.497399, whole genome shotgun sequence.
ACCESSION   DAOQJL010000719 DAOQJL010000000
VERSION     DAOQJL010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72010_length_1593_cov_4.497399"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLG78_20045"
     CDS             <1..390
                     /locus_tag="PLG78_20045"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNE25471.1"
                     /translation="SGSSISSFAIYLGCFGKWNKLRNVGRQFFFPAPSVDSALISFER
                     TEMRCNPSMLEDILRSSFHSKRKKISNSWKSAASLRGLREEWLLELAPAAELDPGLRP
                     EEVPPEKFYELARLVDQQFGAQIRAQA"
     gene            complement(381..800)
                     /locus_tag="PLG78_20050"
     CDS             complement(381..800)
                     /locus_tag="PLG78_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE25472.1"
                     /translation="MSQETPFDIVTRHLVMEKDLNAFGNLFGGNMLAWIDEASALYVM
                     EQIGFANFVTVSFDDVNFKAPARRGDQVYISCRILKTGRSSITVETIAEIHAPVTGSH
                     EETIRCKVTFVCLTDNKPFAYFESEDYKKFRQQKTQA"
     gene            complement(847..>1593)
                     /locus_tag="PLG78_20055"
     CDS             complement(847..>1593)
                     /locus_tag="PLG78_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25473.1"
                     /translation="VLPLPRKKMVAFGGSGPYDHTRITRLALERFAERTGLAVRADCS
                     QMLIHGSVISDSERTAYIEELHCDNNIMRCAKKLHELKHLAIHEPDPPRFYYHAGQAL
                     HLVQDFYSHTNWAETFRFTYLLAPIESFKDLPPPEDLQTGVFPDPLQINYEAAFRCLL
                     VPEDKWNRFIPGATHGCLNKDTNRSVRGMRAVQDGFGMTYHELAAEYAIRHSVDLLEH
                     LRSHSIVMNTCLIPDKLSLGCNQSINELLQ"
BASE COUNT          363 a          371 c          413 g          446 t
ORIGIN      
        1 tctggttcga gcatcagttc ctttgcaata tatctgggat gttttggaaa atggaacaag
       61 ctcagaaatg tgggcagaca gttcttcttt cccgcacctt ccgtggattc tgcgctcatt
      121 tcttttgaaa gaacggaaat gagatgcaac ccttctatgc tggaggatat ccttcgctcc
      181 tcttttcatt caaagagaaa aaagatttcc aattcgtgga agtctgcggc cagtctccgt
      241 ggtcttcgtg aagaatggct actggaactt gcgccggcgg cagagctcga tcccgggctt
      301 cgcccggaag aagttccgcc ggagaaattc tatgagctcg cgcgccttgt ggatcaacaa
      361 tttggagcgc agattcgggc tcaggcctga gtcttttgtt gtctgaattt tttgtagtct
      421 tcactttcaa aataagcaaa cggcttgttg tccgtgagac agacgaaagt taccttgcag
      481 cggatggttt cttcgtggct gcctgtgacc ggtgcgtgga tttcggcaat cgtttccact
      541 gtgatggaag atcttcctgt ttttagaatg cgacatgaga tataaacctg gtctccgcgt
      601 ctggcaggag ctttgaaatt cacgtcgtca aaacttaccg ttacaaagtt tgcaaatcca
      661 atctgttcca ttacatagag tgcggatgct tcgtcgatcc aggcgagcat attgccaccg
      721 aacaggtttc cgaatgcgtt gaggtctttt tccatcacca gatggcgtgt cacaatatcg
      781 aaaggtgttt cctggctcat agccagaggt ttttgcgacc gcgtacgttc gcaaggaaat
      841 cctgcatcat tgaagcagtt cgtttatact ttgattgcat cccagggaaa gcttgtctgg
      901 aatgaggcaa gtattcatca caatgctgtg cgatctgaga tgctcgagca agtccacgga
      961 gtggcgtatg gcgtattcag ctgccagttc atggtaagtc atcccaaagc cgtcttgcac
     1021 cgctctcatt cctctcacag aacgatttgt atctttgttg agacatccgt gagtagcacc
     1081 tggaatgaaa cggttccact tatcttcggg gacaagcaag catctgaaag ccgcttcgta
     1141 gttgatttgc agtggatccg gaaaaactcc tgtctgcaaa tcttcaggcg gtggcagatc
     1201 tttaaaagat tcaatgggtg ccagcagata cgtgaatcga aacgtttccg cccagtttgt
     1261 gtgtgagtaa aaatcctgga ccagatggag agcctgtccc gcgtgatagt agaatctggg
     1321 cgggtcaggc tcatgaattg ccaggtgttt gagttcgtgc agcttcttcg cgcacctcat
     1381 gatgttgtta tcgcagtgga gttcttcgat atatgctgtg cgttcgctat cactgatgac
     1441 ggatccgtgt atgagcatct gactgcagtc tgcgcgaacg gccagtccgg ttctttctgc
     1501 aaagcgttcc agtgccagcc ttgtgatgcg agtatgatca tagggccccg atccgccaaa
     1561 ggctaccatc ttcttgcgtg gaagcggaag gac
//
LOCUS       DAOQJL010000720         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_72012_length_1593_cov_4.234720, whole genome shotgun sequence.
ACCESSION   DAOQJL010000720 DAOQJL010000000
VERSION     DAOQJL010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72012_length_1593_cov_4.234720"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLG78_20060"
     CDS             <1..476
                     /locus_tag="PLG78_20060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE25474.1"
                     /translation="FGAAVLGTVGSEDRMDSTIIGDSVNLAARMEGLTKQYGCSLMIT
                     EDAASRLKKGSFKLREADTVRVKGKKKPIRIFDVFEADPAPVKKLKARTAPLLSSGIT
                     LYHKKRFEQALKTFEKMHGIDKKDPLASLYIERCKLNIKNGVPQDWDGSATLTEK"
     gene            480..>1593
                     /locus_tag="PLG78_20065"
     CDS             480..>1593
                     /locus_tag="PLG78_20065"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE25475.1"
                     /translation="MNFTSFHFVIFFFITLTLGHILKNRTQRVFLWLASYYFYGVLEP
                     FYLVLIMISTSWDYFSGIAIDSAIRRENGQEMTAFQQRLSRFPKKTWVILSVIFNLCL
                     LGYFKYTNFGIQVFNDLQPLGDTVFAWPAQQILLPVGISFYTFMSMSYTVDVYRGVVE
                     ARKNFIDFALYICFFPHLVAGPIVRPKTFFSKLDNRLPVDQEAVMIAVSRIVVGFFRK
                     MVLADNIAPIVNQVFAQPQAYSTADTWLAALAFGIQIYFDFAGYTDIARGVARLFGFE
                     FEVNFNYPMAASSIKDHWERWHISLASWVRDYIFIPLGGSRVSAPRLYFNMFASWFFV
                     GIWHGPAYHFVIWGVWQAVMLSIHRMWSMSAIGLKLN"
BASE COUNT          388 a          414 c          372 g          419 t
ORIGIN      
        1 attttggcgc ggcagtgctc ggaactgttg gatcagaaga caggatggat tcaacgatca
       61 tcggagattc agtgaacctg gccgcgcgta tggaaggact gacaaagcaa tacggctgca
      121 gcctcatgat cacggaagat gctgcttcca gattgaagaa aggatctttc aaacttcgcg
      181 aagcggacac tgtgcgagtc aaaggcaaga aaaagccaat ccgcatcttt gatgtatttg
      241 aagcagatcc agcccctgta aaaaaactca aagcgcgcac ggcacccctt ctctcatcgg
      301 gcatcactct ctatcacaag aagcgttttg agcaggccct caaaaccttt gaaaagatgc
      361 atggcatcga caaaaaggat ccgcttgcgt ccctgtacat tgaacgctgc aaactcaata
      421 tcaaaaatgg cgtcccgcaa gactgggatg gatcggccac attgacagag aaataattca
      481 tgaacttcac ctcctttcat ttcgtaatct ttttctttat cacgctaaca ctcggacaca
      541 tactgaagaa ccgcacacag cgcgtgtttc tgtggcttgc gtcctactat ttctacggcg
      601 tactggagcc tttctattta gtgctcatca tgatttctac ttcctgggac tatttttcag
      661 ggattgccat agactcagca atacgccggg aaaatggcca ggagatgaca gccttccaac
      721 aaaggctctc ccgtttcccc aagaaaacct gggtgatcct ttctgttatt ttcaacctct
      781 gcctgctggg atacttcaag tacacgaatt ttggaattca agtattcaat gacttgcaac
      841 cgttaggcga cactgtgttc gcctggcccg cccagcaaat ccttctgccg gttggtattt
      901 ctttttatac cttcatgtct atgtcctaca cggtggatgt ctaccgcggc gttgtagaag
      961 ccagaaagaa ctttatcgat tttgccctgt atatctgctt tttcccgcac ctggtggcgg
     1021 ggccaatcgt tcgcccaaag acattcttct ccaagctgga caatcgcctc cccgtagacc
     1081 aggaagcggt tatgatcgca gtgtcgcgca ttgtggtcgg gttttttcgc aagatggtcc
     1141 ttgcagataa cattgcacca atcgtgaacc aggtcttcgc acaacctcag gcatactcca
     1201 cggcagacac gtggctggcc gctcttgctt ttggcattca gatttacttt gatttcgcag
     1261 gctatacgga cattgcgcgc ggtgttgcga gactctttgg atttgaattc gaagtgaatt
     1321 tcaactaccc catggccgcg agttctataa aagaccactg ggaacgctgg catatctctc
     1381 tggcaagctg ggtgcgcgac tacatcttca taccgttagg cggaagcaga gtaagcgcac
     1441 cacgtctgta cttcaacatg tttgcctcct ggttcttcgt cgggatctgg cacggccccg
     1501 cctatcactt cgtaatctgg ggagtctggc aggcagtgat gctttccatc catagaatgt
     1561 ggagtatgtc agccattgga ctgaaactca acg
//
LOCUS       DAOQJL010000721         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_73946_length_1572_cov_3.152933, whole genome shotgun sequence.
ACCESSION   DAOQJL010000721 DAOQJL010000000
VERSION     DAOQJL010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73946_length_1572_cov_3.152933"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLG78_20070"
     CDS             <1..497
                     /locus_tag="PLG78_20070"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE25476.1"
                     /translation="TALRATSSLPGIAPPLFMDGQLFVDGGLLNNLPVDAMQDFGAGK
                     IIAIDVSSNAQHEDEEYGKFMGAQTASVGPSFFRVLANKLRPRARRYKLPSLASVLIR
                     STMIGSVKRVRNARREADIYARLPLGGFGLLDWKAMDDLIKIGYDYAVENMPLWKKSL
                     GLSD"
     gene            512..910
                     /locus_tag="PLG78_20075"
     CDS             512..910
                     /locus_tag="PLG78_20075"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE25477.1"
                     /translation="MREPRGFTFPSLITGITSAMLLIDLAGLVSHFNVDLRMGFLAYV
                     FIGTETHRMHHSADTISSKNFGNTLALWDIVFGTFQYASGQRPERIGIADPETHPRSE
                     DLFRVLAFPFGGPPGLAEKEVHPLDEGASV"
     gene            914..1162
                     /locus_tag="PLG78_20080"
     CDS             914..1162
                     /locus_tag="PLG78_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25478.1"
                     /translation="MKSYKFTLSYPEKKSPALENKIGPLVRKHNGKFESKFNFADNET
                     IVTVTLPKADKAGFEADLKDPKYNFLHLESVVFAMDEE"
     gene            1159..>1572
                     /locus_tag="PLG78_20085"
     CDS             1159..>1572
                     /locus_tag="PLG78_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE25479.1"
                     /translation="MKLFIKFSISWATHSRFSFVWITVLSFWIAAPLRAEEWNFSTTR
                     GVVAGVLKWKDSGLESFSDRFRKDAELHSLLIRRGVPASNLKLLLDGAATRAGIESAV
                     REAALNAPADSVFYFYYAGHGIKDASGSYLANYDID"
BASE COUNT          378 a          378 c          413 g          403 t
ORIGIN      
        1 ggactgctct gcgcgcgaca tcgtcgctgc cgggaatcgc gcctccgctg ttcatggacg
       61 gccagctatt tgtggacggg ggtttgctga acaaccttcc ggtggatgca atgcaggatt
      121 ttggcgccgg gaaaatcatt gccatcgacg tttcatccaa tgcccagcac gaggatgaag
      181 aatatggaaa attcatgggc gcgcagactg cctcggtggg accgtctttc tttcgcgttc
      241 ttgccaacaa gcttcgaccc agggctcggc gttacaaact tccatcactc gccagcgtcc
      301 tgattcgatc tacaatgatt ggcagcgtta agagggttcg caatgctcga agagaggcgg
      361 acatctacgc acgtctccct ctcggcggct ttggactgtt ggactggaag gccatggacg
      421 atttgattaa gataggttat gactacgcgg tcgagaatat gccgctgtgg aaaaaaagtc
      481 ttggattgag tgactgaaca gggcgactca cgtgcgtgaa ccgcgcggct tcacgttccc
      541 ttctttgatc acaggcatta ccagtgccat gctactgatt gatctggccg ggcttgtttc
      601 tcatttcaat gtagatcttc gcatgggatt tcttgcatac gtattcattg gaaccgagac
      661 acatcggatg catcacagtg cggatactat ttcatccaag aattttggga acacccttgc
      721 attgtgggac attgtgtttg gaacctttca atacgcgtca gggcagagac ctgagaggat
      781 tggaattgcc gatccagaga ctcatccgcg atcagaagat ctctttcgcg tgcttgcctt
      841 ccccttcggt ggacctccag gattagcaga aaaagaagtg catccgttag acgagggtgc
      901 atccgtgtga aacatgaagt cctataagtt cactctttcc tatccggaaa agaaatctcc
      961 tgctctcgaa aacaaaattg ggccgctggt tcgcaagcac aacggaaagt tcgagagcaa
     1021 gttcaatttc gctgacaatg aaaccatcgt gactgtcact ctaccgaaag cggacaaagc
     1081 cggtttcgag gcagacctga aagatccaaa atacaacttt ctgcatcttg aaagtgtagt
     1141 gtttgccatg gacgaggaat gaaattgttt ataaaattta gtatttcctg ggccacgcat
     1201 tcccgtttct cattcgtctg gattaccgtt ttatcattct ggatcgccgc gcctcttcgt
     1261 gcggaagaat ggaatttttc aaccactcgg ggagtagttg cgggagtgtt gaagtggaaa
     1321 gattcaggat tggaaagttt ctcggatcga ttccgcaaag acgcagaatt gcactcgctc
     1381 ttgattcgcc gcggcgttcc tgcatcgaat ttgaaactgt tgttagacgg agccgccaca
     1441 cgcgcaggta ttgagagtgc ggtaagggaa gcagccctga acgcaccggc ggacagcgtt
     1501 ttctattttt actatgccgg acacggaatc aaggatgctt cagggtccta cctggccaac
     1561 tacgatattg at
//
LOCUS       DAOQJL010000722         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_74406_length_1567_cov_3.341931, whole genome shotgun sequence.
ACCESSION   DAOQJL010000722 DAOQJL010000000
VERSION     DAOQJL010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74406_length_1567_cov_3.341931"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLG78_20090"
     CDS             <1..525
                     /locus_tag="PLG78_20090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE25480.1"
                     /translation="EVGADAMQLFVAEPLTLSPGDPVVVEFKIEKNQFSFEAKTQSMT
                     GSLLSLSKPAAIKRSRLREGHRLAVDMKMAYTPWTESGRFETTLVDLSEMGVRMIGRK
                     QLKKGMLVSIDFYVKEDKIRVLCQGQVAWWRATPDNDQLFETGVEFTTISNETRKRLT
                     KFILRKNGGEEKGE"
     gene            518..1312
                     /locus_tag="PLG78_20095"
     CDS             518..1312
                     /locus_tag="PLG78_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002749959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE25481.1"
                     /translation="MSSLNYVVPPALEEAVHVSEITGRPLLLKGEPGTGKTLLAESLA
                     QSRGLGIFRWHVKSISQAKDGLYFYDALSRLNDSRFAENSEKIKNIENYIQLGALGQA
                     FESESPCIVLIDEIDKADAEFPNDLLLELDQMEFSITETGRRVQAKRRPLAIITSNNE
                     KELPDAFLRRCIFHYIDFPSSDFMKVIVRSHYPDIDHKLLESSLEIFYKLRALGDLKK
                     KPSTSELIDWMRILIHEGAALSDGKIPWVGALIKNEEDLREVKSRF"
     gene            1320..>1567
                     /locus_tag="PLG78_20100"
     CDS             1320..>1567
                     /locus_tag="PLG78_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HNE25482.1"
                     /translation="MFTNFFYRLRKAGLRCGTGELLDLLRALDGYADLEIPLTPERFY
                     FISRACLAKDEKYFDAFDRTFAATFQESLESEDAFRKIL"
BASE COUNT          445 a          361 c          390 g          371 t
ORIGIN      
        1 gaagtaggcg cggatgcaat gcaactgttc gtcgccgaac ccctcactct ttctcccggg
       61 gatccagtgg tcgttgaatt caaaattgaa aagaatcaat tttcctttga ggcaaaaacg
      121 cagtccatga ctggttctct attgtccctc agcaaacccg ccgctatcaa acgctcgaga
      181 ctgcgcgaag gccacaggct tgcagtcgac atgaaaatgg catacactcc gtggacagaa
      241 tccggcagat ttgaaaccac tctcgtcgac ttgagcgaaa tgggcgtgcg aatgattgga
      301 agaaagcaat taaagaaagg catgctggtc tcgatcgatt tttatgtaaa agaggataag
      361 atcagagttc tttgccaggg tcaggtagcg tggtggagag cgactccgga caatgatcag
      421 ctattcgaga cgggtgttga attcactacg atttcaaacg aaacacgaaa gcgcctgact
      481 aaattcatcc tcaggaagaa cggcggagaa gaaaaaggtg agtagtctaa actatgttgt
      541 tccacccgcg ctcgaagaag cggtgcatgt atcagaaatt accgggcgtc cgcttctact
      601 caaaggagaa ccggggacgg gaaagacttt gcttgcagaa tctctggcac agtctcgggg
      661 gctgggcatc tttcgctggc atgtaaagtc catttcacag gcaaaagacg ggctgtattt
      721 ctacgatgcg ctttctcgtc tcaacgattc caggtttgct gaaaattctg aaaaaataaa
      781 gaatattgag aattatatac aattgggcgc tctgggtcag gcttttgaga gcgaatcacc
      841 gtgcattgtc ctcatagatg aaatcgacaa ggcagatgcg gagtttccca atgaccttct
      901 actagaactc gaccagatgg agttttccat tacggaaaca ggaagaagag tgcaggcaaa
      961 aaggcgccca ctggccatca ttacatccaa caacgaaaaa gaattgccgg atgcgttttt
     1021 gcgcagatgc atatttcact acatagactt cccgagctct gacttcatga aagtcattgt
     1081 tcgctcgcat tacccggaca tcgaccataa gctcctcgaa tcttctctgg aaattttcta
     1141 caaactaaga gcattgggcg atctgaaaaa gaaaccgtcc acatccgagc tcatagactg
     1201 gatgcgaatc ctgatacatg aaggggccgc tctttcggac ggaaagatcc cgtgggtcgg
     1261 ggcactcatc aagaacgaag aggatttgcg agaagtcaaa agcaggttct aggaccgcta
     1321 tgttcacaaa ttttttttac agactgagga aagcgggact gcgctgcggc acaggcgaac
     1381 tcctggattt gctgcgcgct ctagacgggt atgcagatct ggagattcca cttacaccgg
     1441 agagattcta ttttatttcg cgcgcatgcc ttgcaaaaga cgaaaagtat ttcgacgcat
     1501 ttgatcgcac atttgccgcc accttccagg agtctctgga atcagaagat gcgtttcgaa
     1561 aaattct
//
LOCUS       DAOQJL010000723         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_76038_length_1550_cov_5.102341, whole genome shotgun sequence.
ACCESSION   DAOQJL010000723 DAOQJL010000000
VERSION     DAOQJL010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76038_length_1550_cov_5.102341"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1550)
                     /locus_tag="PLG78_20105"
     CDS             complement(<1..>1550)
                     /locus_tag="PLG78_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE25483.1"
                     /translation="LWLHLKPVRRTQVFLLMGLMVAVSAAEVISIGAILSFLAVLTDP
                     GRTFHHPRLQFLWELTGIQSPEQMVLPMTIAFACAALVTGGARLLFLWANTRLCNAIG
                     ADLAYDVYRRTLFQPYFVHVGRNSSEVATATSKAKGLVNQTISPLLTIFGSLCVIVSI
                     VSFLVTIDPVVSLSAVAGFSTIYFVIAIFSKNSLDTNGRILARELTRATQTMSEGLGG
                     IRDILLDGSQDFYSGIFRTSDRRLNRANANATFLAGTPRFAIEAVAMVLLAGFAYALT
                     RSPGGLNQHIPLLGAMALGAQRILPVMQATFVSWTNVKISQSTMREVLGFLDQPLPAY
                     ATEPPPIPMAFKDRITLDHIGFRYSEEGPWVLRDVTMEIPHGSRVGIVGITGSGKSTL
                     LDILLALLVPTEGRLLVDGVTIDSTTMRAWQANVAHVPQMIYLSDTSIAENIAFGVPA
                     DQIDMDRVKRAASQAQISSHIETLQAGYQTFVGERGIRLSGGQRQRIGIARALYKQAS
                     VIVFDEAT"
BASE COUNT          411 a          402 c          419 g          318 t
ORIGIN      
        1 tggtcgcctc gtcaaagacg ataacggatg cttgcttgta gagtgctctt gcaatgccga
       61 tcctctggcg ttgtccacct gagagcctga tgccgcgttc tccgacaaaa gtctggtatc
      121 cggcctgcag tgtttcaatg tgagagctga tctgggcctg cgacgctgcg cgcttcacgc
      181 gatccatgtc gatctgatct gccggcacgc caaaagcgat attctccgcg atcgacgtat
      241 cagaaagata aatcatctga ggcacatggg ccacgttcgc ctgccacgca cgcatcgtag
      301 tgctatcaat ggttacgcca tcgacaagaa gtcgaccttc cgtggggaca aggagtgcca
      361 gaagaatatc cagcagtgta ctttttccgc ttccagtgat gccaacaatt cccactcgag
      421 atccatgagg gatttccatc gtaacgtcgc gaagaaccca cggaccttct tcggaatagc
      481 gaaaaccaat atgatctaac gttatgcgat ccttaaaggc cattggtatc ggtggtggct
      541 cagtagcata ggccgggaga ggctgatcga ggaagccaag cacttcccgc atagtagact
      601 ggctaatttt cacgttggtc caggagacga aagttgcctg catcacaggc agaatcctct
      661 gggccccgag agccatcgca ccaaggagtg gaatatgttg attgagacct cctggagagc
      721 gcgtgagcgc gtaggcgaag ccagcaagaa gaaccatggc gacagcttca atggcaaacc
      781 tgggcgtccc cgcaagaaaa gtggcatttg cgtttgcacg attcaaacgc ctatctgacg
      841 tgcgaaaaat gccactgtag aaatcctggc tgccgtcgag aaggatgtcg cggataccgc
      901 caagaccctc gctcatagtt tgagtggctc gagtgagttc gcgagccaga atacgtccat
      961 tcgtatccag actattcttt gaaaaaatag caatcacgaa atatatcgtt gagaaccctg
     1021 cgacagcgga gagtgaaacg acagggtcta tggttacaag aaaagaaacg atggaaacaa
     1081 tgacgcagag ggatccaaaa attgtgagaa gcggcgaaat tgtctgattc acgagacctt
     1141 tcgccttcga tgtcgcagtc gctacttcac tggaattgcg acccacgtgc acaaaatacg
     1201 gttggaacaa agtgcggcga tacacatcgt acgcaaggtc cgcaccgatc gcattgcaga
     1261 gccgcgtatt cgcccacaaa aacaatagac gtgcgccacc tgtcaccagg gcggcacatg
     1321 caaaggcaat cgtcatagga agaaccattt gctccggcga ctgaatcccg gtcagttccc
     1381 ataggaattg gagccgagga tggtgaaacg ttcgccctgg atctgtgagg actgccagga
     1441 aggagaggat cgctccgatg ctgatcactt cggcggcgga tacagcgacc atgagcccca
     1501 tcaagaggaa cacctgagtt ctgcgaacag gcttcagatg cagccagagt
//
LOCUS       DAOQJL010000724         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_77112_length_1539_cov_4.498652, whole genome shotgun sequence.
ACCESSION   DAOQJL010000724 DAOQJL010000000
VERSION     DAOQJL010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77112_length_1539_cov_4.498652"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..151
                     /locus_tag="PLG78_20110"
                     /pseudo
     CDS             1..151
                     /locus_tag="PLG78_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            complement(240..662)
                     /locus_tag="PLG78_20115"
     CDS             complement(240..662)
                     /locus_tag="PLG78_20115"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNE25484.1"
                     /translation="MDLSTQSINQYGLQSAQDSRGADSIRLLKQRDEKRSTFADMVNE
                     HARPIIPKSKEKLMEEVRSNPENKKLYEAAQDFQAIFLGQMLSAMRKNLHKDSDMMYG
                     GRTEEIFQDFLYDEYAKMMSKSPGFNLSDEIYKQLKGE"
     gene            complement(674..>1539)
                     /locus_tag="PLG78_20120"
     CDS             complement(674..>1539)
                     /locus_tag="PLG78_20120"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNE25485.1"
                     /translation="IDSGSLQARNVAAVLVTAEVPAFANRGDKLNATVSSIGDAKSLE
                     GGVLIQTPMQAGDGNMYAVAQGVVTLGGQTGNHQQKGRTVGTVMSGVNVEKDIVSSYV
                     FEQIQDKNEKGEKIDPPRRIQKIRITLNDFDFQTARDARDKIEKKFPESRPMIDNGSI
                     SVEIPAGKDVASFIAEVEDIRVSPRLKARIVVNERTGAIVMGGDVRVEPVAVSRGGMD
                     LIVSGNPYPNGVQLTRQDEKKPTMQPFSGASVQEIMDALNAMGASTRDIVAILEALRD
                     SGALHAELIVQ"
BASE COUNT          341 a          413 c          349 g          436 t
ORIGIN      
        1 aatcgcggat tccactgctg cagaaattac aaaaaaaaca tccattccaa ctattggaat
       61 tggttccggc aattctacgg atggtcaggt gcaggtactc cacgatgtgc tggggctcga
      121 tcctgatttt cgtcccaaac atgcaatgag aatgcgtgac atcggtagcg atttgatctc
      181 cgcctgctcc gaatttcaca attccattca gatcaagtga attgtaaacg tcaaaagagt
      241 tattcgcctt tcaactgctt atagatctca tcagacaggt tgaacccagg agatttggac
      301 atcatttttg cgtattcgtc gtatagaaaa tcctggaaga tttcctctgt gcgaccacca
      361 tacatcatat cactgtcttt gtgtaagttc tttctcatcg cagaaagcat ctgaccaaga
      421 aaaatcgcct gaaagtcctg agccgcttcg tagagcttct tattctctgg attggagcga
      481 acctcttcca taagcttttc ttttgacttc ggaatgattg gccttgcgtg ctcgtttacc
      541 atgtctgcga aggtggatcg cttttcatct ctctgcttca agaggcgaat gctgtctgca
      601 ccccgcgaat cctgcgcgct ctggagtcca tactgattga tcgattgtgt gcttaagtcc
      661 atgtcattct tccttattgg acgatcagct ccgcatggag tgcgcctgaa tcgcggagag
      721 cttctaatat ggctacaatg tctctcgtgc ttgcacccat tgcgttgagt gcatccatga
      781 tctcttgcac ggaagctccc gaaaatggct gcatggttgg ctttttttcg tcctgtctgg
      841 tgagctggac tccattcgga tacgggttgc cagaaacaat gagatccatt ccaccacggc
      901 tcacggccac gggctccacg cgcacatctc cacccattac aatggcacct gtcctttcat
      961 tgaccacgat gcgcgccttg agtctgggag aaacgcgaat gtcttccact tccgcgatga
     1021 aagaagccac gtccttgccc gcaggaatct ccacagagat cgacccgttg tcgatcatgg
     1081 gtctggactc agggaatttc ttttctattt tgtcgcgcgc atcgcgcgcg gtctggaagt
     1141 caaagtcgtt gagagtaatg cgaatttttt gaattctacg aggtggatcg atcttctcgc
     1201 ctttctcgtt cttatcttga atttgttcaa acacgtaact cgatactatg tctttctcaa
     1261 catttacgcc actcatcacc gtgcccactg tgcggccctt ctgctggtga tttcctgtct
     1321 ggccaccgag agtaacaact ccttgagcca cagcatacat atttccgtcg ccggcctgca
     1381 tgggagtttg aatcagcacg ccgccttcga gagacttcgc atctccaatc gacgaaaccg
     1441 tagcgttgag cttgtctcca cggtttgcga acgccggcac ttctgcagtg actagaaccg
     1501 cagccacgtt ccgagcctgg aggctgcctg aatctattc
//
LOCUS       DAOQJL010000725         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_77755_length_1533_cov_3.099459, whole genome shotgun sequence.
ACCESSION   DAOQJL010000725 DAOQJL010000000
VERSION     DAOQJL010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77755_length_1533_cov_3.099459"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLG78_20125"
     CDS             complement(<1..259)
                     /locus_tag="PLG78_20125"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE25486.1"
                     /translation="MKLRLGAGYGAAETGIAAAEVMLQLYLLNFYIGQGLSPFLTGLA
                     LACAVALDAFADPLVGGISDRARPEMGRRRAFILGGSIFLSI"
     gene            complement(256..1008)
                     /locus_tag="PLG78_20130"
     CDS             complement(256..1008)
                     /locus_tag="PLG78_20130"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE25487.1"
                     /translation="MSTLILVFAVQAFCFIFASVFQTDKLTDFAYGMTFVLLASIFLL
                     QAGENTLSWILCSCIILWGLRLSGYLFVRILKIGKDERFDGRRENFWRFAFFWIFQAI
                     VIWVILLPVIVAVQSSAQPGVLSYAGVAVFLFGLVYETIADAQKFQFRNRPENKGKWI
                     ESGLWKYSRYPNYFGEVVLWWGVFLIAIPALSAAAWFTIAGPICITFILLKVSGIPLL
                     EEGHKKRYGKNPAWLAYAKRTSTLFPWFPEKQ"
     gene            1233..>1533
                     /locus_tag="PLG78_20135"
     CDS             1233..>1533
                     /locus_tag="PLG78_20135"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HNE25488.1"
                     /translation="MSQGAPGELTLSGRYNLDYNRQYLGTGPAIFERAKAAIRQWKMF
                     DLSWIDLVPLETPLEAGRTVAVVARHLGFYSLNASRILYTVEETGEMERFGFAYGT"
BASE COUNT          424 a          371 c          379 g          359 t
ORIGIN      
        1 caatggaaag aaagatggag ccacccagaa tgaatgcacg tcttctgccc atctccggac
       61 gagctctatc agagatcccg ccaactaacg ggtccgcaaa cgcatccagc gctacggcac
      121 aggcaagtgc cagtccagtc agaaaagggc tcaatccctg gccaatatag aaattcagaa
      181 gatagagctg cagcataact tctgctgcag cgattcctgt ttcggccgct ccgtaacctg
      241 ctccgagtcg cagtttcatt gcttttcagg aaaccacggg aagagtgtgc tcgttcgctt
      301 tgcgtaggcc agccatgcag gattctttcc gtaccttttt ttgtgacctt cttccagcag
      361 cggaatgccg ctcactttca gaaggatgaa cgtgatgcaa atgggacctg cgattgtaaa
      421 ccatgctgct gcggataacg cagggatcgc aataaggaaa actccccacc acaaaacgac
      481 ttcaccaaag tagttgggat atcttgagta tttccagagt ccgctctcta tccatttgcc
      541 tttgttttcg ggcctgttgc gaaattggaa tttttgcgcg tccgcgatag tttcgtatac
      601 caggccaaag agaaacacag caacgcccgc atagctcaag acgccaggtt gtgcacttga
      661 ttgcacggcg actatgactg gaagcaagat gacccagatg acaatcgctt gaaagatcca
      721 gaagaatgcg aatctccaga aattctctct tctcccgtca aatcgttcgt ctttgccgat
      781 tttcagaatt cgaacaaaaa gatatccgga aagtctcaag ccccacagga tgatgcaact
      841 gcaaagaatc catgacagcg tattttcgcc tgcctgcaaa agaaaaatag acgcgagtag
      901 aacaaaggtc attccgtacg caaaatcagt cagtttgtct gtttggaaaa cagatgcaaa
      961 gatgaaacag aatgcctgga ctgcgaacac cagaatgagg gtagacaaca ggttccagga
     1021 atcgaaagaa aagaaaggca ggaagtgcat cctggtaaac taatggaaat gcgttccgct
     1081 gtctctatat tttgtcgtag gaaacgcaaa aacattggac aatatgtcga aggccaaatc
     1141 ggatgatgat gctgcgtgct ccccgtgaat cggatgtgct gagatttctt gaggtttctg
     1201 ctacagaccg tttctcgtat gaggaaaccg ggatgagtca aggagctcct ggggaactca
     1261 ctctttccgg tcgctataac ctggactaca atcgacagta cctcggaacg gggccggcta
     1321 tttttgaacg cgcaaaggca gcgattcgcc aatggaaaat gtttgatctg agctggattg
     1381 atctggttcc gctagaaacg cctctggagg cgggtcggac tgttgccgtc gtggccagac
     1441 atctggggtt ctattcactc aacgcatccc gaattctcta tactgtagaa gaaacagggg
     1501 aaatggaacg atttggtttc gcctatggca cgt
//
LOCUS       DAOQJL010000726         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_78760_length_1523_cov_4.935967, whole genome shotgun sequence.
ACCESSION   DAOQJL010000726 DAOQJL010000000
VERSION     DAOQJL010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78760_length_1523_cov_4.935967"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLG78_20140"
     CDS             <1..668
                     /locus_tag="PLG78_20140"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNE25489.1"
                     /translation="IKQRGTKALVTMPLSKHHVSLSHPAFRGHTDYLADEFSSKVIML
                     MHGKALSVIPLTVHVPLSHVPAELHARMNDADLIRILKILHGWKEYSGTRWALCGLNP
                     HAGENGLLGREEIDFMNIIADRWKAEGLPIEGPLPADGIFQEYHHGRYRLVLSAYHDQ
                     GLAPFKALEGRAGVNCTIGLPFVRTSPDHGTAFEIAGKGVADSASFAAAFELAQASEP
                     LWI"
     gene            665..1183
                     /locus_tag="PLG78_20145"
     CDS             665..1183
                     /locus_tag="PLG78_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25490.1"
                     /translation="MNAILFPLFAIWGIFSIVLLFQKRIEWHLRISAMLICAFYIVLF
                     YPEAHQSFESYRDSFRTTLGALLTSVLPFVGLLLLVFWPFVILISFYSIHASLARGLL
                     RIFTVLTLLFWFLQFALPRMGWHLDPILSRVPEKVHVPEIQTPDVKMPDLKIPEVKLP
                     SLKGDSGSQQKK"
     gene            complement(1134..>1523)
                     /locus_tag="PLG78_20150"
     CDS             complement(1134..>1523)
                     /locus_tag="PLG78_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25491.1"
                     /translation="EAVDLKRFHHQWQPDEIAGGLDFTLGLLLFAPFLLDATLTIFRR
                     LSDRHNIFSAHRTHLYQLLRQNGWSVSRVLVLYQIANLVCAGIAVWRERANVTADYFA
                     AGLFLFAVVVTFIFSAATRYLLSKKAA"
BASE COUNT          354 a          376 c          377 g          416 t
ORIGIN      
        1 ggatcaaaca acgtggaaca aaagcactcg tcaccatgcc tctttccaag catcacgtga
       61 gtttgtctca tcccgcattt cgcggacaca cagattacct ggcggatgaa ttcagttcca
      121 aggtgatcat gttgatgcat ggaaaagctt tgagtgtcat tccacttaca gttcatgtac
      181 cgctgagcca tgtgccggct gagttgcatg caagaatgaa cgatgcggat ttgatccgca
      241 ttttgaaaat ccttcacggt tggaaagagt actctggcac gagatgggct ttgtgcggcc
      301 tcaatccaca tgcgggagaa aatggacttc ttggccgcga agagatcgat tttatgaata
      361 tcattgcaga tcgctggaaa gcagaaggtt tgcccatcga aggccctctt ccagccgatg
      421 ggatttttca ggaataccat cacggacgtt accggctggt tctttctgcc taccatgatc
      481 aaggccttgc tccattcaaa gctctggaag gcagggctgg cgtgaattgc acgattggcc
      541 tcccgtttgt tcgcacaagt cccgatcacg gaaccgcatt tgagattgca ggaaaggggg
      601 tggcagattc agcttccttt gcggctgctt ttgagcttgc ccaggcttca gaaccgctat
      661 ggatatgaac gcgattctct ttccactttt tgcgatctgg ggaatcttct ccatagttct
      721 tctttttcag aaaagaatcg aatggcatct tcgcatcagt gccatgctga tttgtgcctt
      781 ttatattgtc cttttctatc cggaggctca tcaatcgttt gagagctaca gagattcgtt
      841 tcgtactacg ttaggcgctc tgctcacttc tgtattgcca tttgtaggct tgctgcttct
      901 ggtattctgg ccattcgtca ttttgatttc tttctacagc attcacgcaa gcctcgcgcg
      961 cggactcctt cgcattttta ctgtgctcac tctactgttc tggtttcttc aattcgcttt
     1021 gcctcgcatg ggttggcatc tcgatccaat cctttcgcga gttccagaaa aagtgcatgt
     1081 ccctgagatt caaactccgg atgtaaagat gccggatctg aaaattccgg aagttaagct
     1141 gccttctttg aaaggagata gcgggtcgca gcagaaaaaa taaaagtcac aacgactgcg
     1201 aacaagaaca gcccggccgc aaagtaatct gcggtgacgt ttgctctttc cctccagact
     1261 gcaatgcccg cgcacacgag gtttgcgatc tggtagagta ccagtactct gcttacggac
     1321 catccatttt gccgcaggag ctggtagaga tgagttctgt gcgcggagaa gatattgtgt
     1381 ctgtcagaca atcgcctgaa gatcgtgaga gttgcatcca gaaggaatgg cgcaaagagc
     1441 aagagcccga gagtaaaatc aagaccgccg gcaatttcat ccggctgcca ctgatggtgg
     1501 aagcgcttga ggtccaccgc ctc
//
LOCUS       DAOQJL010000727         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79584_length_1515_cov_3.418493, whole genome shotgun sequence.
ACCESSION   DAOQJL010000727 DAOQJL010000000
VERSION     DAOQJL010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79584_length_1515_cov_3.418493"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLG78_20155"
     CDS             complement(<1..643)
                     /locus_tag="PLG78_20155"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE25492.1"
                     /translation="MKRIAVETLGCRLNLFESDGILQQFLSSGRYALSDEKPDLVIVN
                     TCTVTDQADHRNRNIIQKTIKNHPGAHVLVTGCYAQTDPEDLKSIPGVFLVAGNDQKS
                     KLFSLYEEKARGDSSPFSVSDPFAYGNVLPQGHTRAYLKIQDGCDRKCTYCKIPAARG
                     RGVSRSLEDILDHARELEEKGIPEIVLTGVNLGWYKDRGVRFGKVVAEILQILR"
     gene            complement(640..1395)
                     /locus_tag="PLG78_20160"
     CDS             complement(640..1395)
                     /locus_tag="PLG78_20160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE25493.1"
                     /translation="MKKTATLLILFAAVALFAEVPNGYNEALALYSQGKYEESLAKVR
                     SVFEENKTSYHLRMLAAANHAHAGRFNDAAAHLQYLLKDHPDRPEPAALYAAILRKQG
                     QTGDAQGLIARAMQQDPKNMNFVLEAARIHYRAGNFAAARKHIENVLLAAPGNQEALA
                     LDGLIFLRQGNPENAVFRFRQALGQPSSSKAALSDLYNNMAIALRRSADALEKNGNPG
                     EAASMRTEASDMLARSLEQNPSNAIAKANKEAK"
BASE COUNT          334 a          395 c          388 g          398 t
ORIGIN      
        1 gtcgtagaat ctggaggatt tcagctacca ccttgccaaa gcgaacgccg cgatctttgt
       61 accagcccag attcactcct gttaaaacga tttcagggat gcctttttct tcgagttccc
      121 gtgcgtgatc caatatatct tcgagcgatc tagacacgcc gcgaccgcgc gccgccggaa
      181 ttttacagta ggtgcatttg cgatcgcagc cgtcctggat tttcaaatat gcgcgagtat
      241 gaccctgggg cagcacattt ccataggcaa atggatcact gaccgaaaaa ggactcgagt
      301 ctccccgtgc cttttcctca tagagagaaa atagtttaga cttttgatca ttcccggcca
      361 cgagaaacac acctggaatg gacttcaaat cttccgggtc cgtttgcgcg taacagccag
      421 ttaccaggac atgcgctcct ggatgattct tgattgtttt ctgaatgata tttctatttc
      481 tatgatctgc ctgatctgta acggtgcacg tattgacaat cacgaggtcc ggcttctcat
      541 ctgagagcgc ataacgtcca gaagacagga attgctgcag aattccgtcc gactcaaaca
      601 aattcagacg gcagccgagt gtctcgacag cgatccgttt cactttgctt ctttgtttgc
      661 cttcgcaatc gcgttggaag gattctgttc gagggaacgg gccagcatat cggaggcttc
      721 cgtgcgcatg gatgccgctt ctccggggtt tccatttttt tcaagcgcat ccgcggagcg
      781 gcgaagtgct atcgccatat tgttgtacag atcagacaga gcagccttgc ttgaagaggg
      841 ttgtccgagc gcctggcgaa atctaaagac cgcgttttca ggatttccct ggcgaagaaa
      901 aatcagacca tcgagtgcca gtgcttcttg atttccaggg gcagcgagaa gaacattttc
      961 aatgtgtttg cgcgccgccg cgaagttgcc cgcacgatag tgaatacgcg ctgcttccag
     1021 aacaaagttc atgttctttg gatcttgctg catggcacga gcaattaaac cctgggcatc
     1081 tccagtctgc ccctgctttc gcaagatggc ggcatagagc gcggccggtt ctggtcgatc
     1141 tggatgatct ttcagaagat actgcaggtg tgccgccgca tcattgaaac gacccgcatg
     1201 cgcatgattg gcagcggcaa gcatgcgcag gtgataggaa gttttattct cctcaaatac
     1261 gctgcgcacc tttgcaagag actcctcgta cttgccctgc gaatacagtg ccagtgcttc
     1321 attgtagccg tttggcacct ctgcgaaaag tgctacggct gcgaataata tgaggagagt
     1381 tgctgtcttt ttcatgcttg tccgggaatc aaatctggtt tgggtggaac tggttcgctc
     1441 gcaagaaccg agcgggaccg aatgcgattc ctctctgctt ccttgcgcgc tgcttccttg
     1501 cgttccgtct ccacg
//
LOCUS       DAOQJL010000728         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79619_length_1515_cov_2.552740, whole genome shotgun sequence.
ACCESSION   DAOQJL010000728 DAOQJL010000000
VERSION     DAOQJL010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79619_length_1515_cov_2.552740"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLG78_20165"
     CDS             complement(<1..971)
                     /locus_tag="PLG78_20165"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE25494.1"
                     /translation="MRNKLNSRFGKGVFLFVSRAFNSLAGLFFSLLAGRFLTVEDHGL
                     FASVVARMVIAQAILEAGLQYSMVRFLAPAIRSGAEGEVQSIIRASLWIKTIAMVGLG
                     SLIAIACVILSFWSGPQGVREILPDFGSPLMLWMIFVGGASMSFVSYQEAILVSHGAY
                     NRLSAWIPLTGVLRIALFGGLFLFERGFGPEEIVFAYTFGPVISSVLFFFVFPAKFFF
                     DLSQGTEWRSRLRALVSYNAFIVAASFFSIFSDWMEVLMLQESRDTGVYNAARMPLQG
                     FMILLATMQSMFLPWFAGLQNASEYRAVFLRVYRYLIPGAILLAPGLA"
     gene            complement(980..>1515)
                     /locus_tag="PLG78_20170"
     CDS             complement(980..>1515)
                     /locus_tag="PLG78_20170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HNE25495.1"
                     /translation="NILGIVSKHNGINVSDMLVVVQDRPRHAEIIHGARSAGAGVALI
                     ESGSISAAAEILLRPNGRWTLLYGTYGAPEGLILAYMARVTGGYFAGRISPHHPLFAE
                     QARELGLDDQTLPASEWIVADGCLCLTGIHGNTFLRGVMRQGQGLVTESLLWTADGVE
                     KIVHENGVQMQRADAVF"
BASE COUNT          429 a          430 c          378 g          278 t
ORIGIN      
        1 gcgaggccgg gcgcaagcaa gatggctcct ggaatcaagt aacgatacac tctcaagaac
       61 actgcgcgat attcagaagc gttttgcaat cccgcaaacc acggcagaaa catggattgc
      121 atggtggcca ggagaatcat gaagccctgg agaggcatgc gtgccgcatt gtaaacgccg
      181 gtgtcgcgcg actcctgtaa catcagcact tccatccagt ctgagaaaat tgagaagaag
      241 gaggcggcca cgatgaatgc attgtagctc acaagagcgc gcagcctgct cctccattct
      301 gtaccctggg aaagatcaaa aaagaatttc gcgggaaaaa cgaagaagaa taaaacagaa
      361 gagatcacgg gcccgaacgt ataagcaaaa acgatttcct ccgggccaaa tccacgctcg
      421 aacaagaaca gcccgccaaa gagcgcaatg cgaaggactc cggtcagagg gatccacgca
      481 gaaagtcggt tgtaagcccc gtgcgaaacc aggatcgctt cctgatagct gacgaaagac
      541 atggaagctc cgcccacgaa aatcatccac agcatgagag gagaaccgaa atcaggcaag
      601 atctcacgca cgccctgagg gccggaccag aaactcaaaa taacgcatgc aatcgcaatc
      661 agactaccaa gtccaaccat cgcgatcgtc ttgatccaga gagaagcgcg aatgatgctc
      721 tgcacctctc cctctgcccc acttcgaatc gccggggcga gaaagcgaac catggaatac
      781 tgaagtcctg cctcaaggat tgcctgagcg atcaccatgc gcgcgacaac cgacgcaaat
      841 agtccgtggt cttctactgt aaggaatcta ccggcaagaa gggaaaaaaa caggcctgcg
      901 agcgaattga aagcacgcga aacaaacagg aaaactccct tgccaaatcg actgttgagc
      961 ttgtttcgca attctcccat tagaatacag cgtccgccct ctgcatttgc accccgtttt
     1021 cgtggacaat cttctcaacg ccatctgccg tccagagaag cgattcggtg accagtccct
     1081 gaccctggcg cataacaccg cgaaggaatg tgtttccatg aattccagta agacaaaggc
     1141 aaccgtctgc aacaatccat tcgcttgcag gcagagtctg atcatccagt ccgagctcgc
     1201 gcgcctgttc tgcaaagagc ggatgatgcg gagaaattct acctgcgaag tagccgccgg
     1261 tcacgcgcgc catgtatgcg aggatcaaac cttccggagc tccatacgtt ccatacagca
     1321 gagtccatct accgttcggt cgcaaaagaa tctccgcggc agcgctgatg cttccgcttt
     1381 caatcaacgc gacacccgca cctgcagagc gtgcgccgtg aatgatttcg gcatgccggg
     1441 gtcgatcctg aaccacaacg agcatgtcac ttacgttgat tccgttgtgc ttcgacacaa
     1501 tgccgaggat gtttt
//
LOCUS       DAOQJL010000729         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79780_length_1513_cov_5.111797, whole genome shotgun sequence.
ACCESSION   DAOQJL010000729 DAOQJL010000000
VERSION     DAOQJL010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79780_length_1513_cov_5.111797"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PLG78_20175"
     CDS             complement(<1..1415)
                     /locus_tag="PLG78_20175"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNE25496.1"
                     /translation="MKSGLTTISGKEVGGQKVFEFSTGGPAVVNSRPWNGAYIAEDQI
                     FVLRLDTEVDESSVISGLGFQVAGLREKVGARIVTGSERQAILKTQYNQDEPQRIVLV
                     QSKQTFPYGAKVKLIWGRSITSKSGVAGDQDQILEYTVRNDFTASFSCERENAQADCI
                     PMLPAYISFSSPISKEQALRVVIRGAGKTFSPKLSEDDEDPGISGVSVPGPFPENTDF
                     RVELPGDLRDDANRPLSNANRFPLSFRTASYPPLAKFAAPFGILELKEGGLLPVTLRN
                     IEAPVPVKYHEAGEGMPGRKIKITPENADQVLYWMRRVSSHNREKSIFQGQDVKISDL
                     RLPRPNGDKAFEVIGIPLGGPGFYVIELESRILGNSLLGQDKPLYVPAAALVTNLSVH
                     FKWGRESSLVWVTTLDAGKPVAKAEVAVRDCNGKVWFTGSTDSEGTVRTQSIPENAPS
                     CSKRPFSSGLLVTAVSGGDMGL"
BASE COUNT          363 a          408 c          366 g          376 t
ORIGIN      
        1 aggcccatat ccccgccaga aactgcagtg accaggagcc cgcttgaaaa aggacgcttg
       61 ctacaactcg gcgcgttctc ggggatgctc tgtgttcgca cagttccttc tgaatcagtg
      121 gaacccgtga accatacttt gccattgcaa tcgcgcacgg ccacttccgc tttcgccaca
      181 ggctttcctg catccagagt tgtgacccat acgagagagg attcgcgtcc ccatttaaag
      241 tggacggaaa gattggttac gagggccgct gcaggaacgt acagtggttt gtcctggccc
      301 agaagagaat ttccaagaat gcggctttcc agttcgatca cataaaagcc aggaccgccc
      361 agcggaattc caatgacttc aaatgccttg tctccgttgg gtctgggaag gcgcagatca
      421 gaaatcttta cgtcctgtcc ctggaagatc gacttctctc tgttatgcga agaaactctt
      481 ctcatccagt agagcacttg atccgcattc tccggagtga tcttgatttt tctgcccggc
      541 atcccttctc cggcctcatg atattttaca ggaacaggcg cttcaatgtt gcgaagagtg
      601 acgggcaaca gaccgccttc cttgagttct agaattccaa atggtgccgc gaacttggcg
      661 agaggtggat acgatgccgt gcgaaaggaa agaggaaagc gattcgcatt tgaaagcggc
      721 cgattcgcat cgtcgcgaag atctcccgga agctctacgc gaaagtcagt attctcaggg
      781 aagggcccag gaacggatac accgctgatt cctggatcct catcgtcttc gcttaacttt
      841 ggagaaaaag ttttgccggc tccgcgaatg acaacacgca acgcctgctc ttttgaaatg
      901 ggcgaagaaa aggaaatgta ggcaggaagc atggggatgc aatctgcctg agcgttctct
      961 ctctcacagc tgaacgaagc ggtaaaatca ttgcgcacag tgtattcaag aatctgatcc
     1021 tgatctcctg cgacgccgga ctttgatgta attgatctac cccaaataag ttttactttc
     1081 gcaccgtagg ggaacgtctg tttcgattga acgagcacaa tcctctgtgg ctcgtcctga
     1141 ttgtactgag ttttgaggat tgcctgtctt tcagagccag tgacgattcg cgctccaacc
     1201 ttctctctca gacctgctac ctggaaaccc aggccagaaa ttacgctcga ttcatctact
     1261 tccgtatcca ggcgaagaac aaaaatctga tcctctgcaa tgtacgcacc gttccacggt
     1321 ctgctattga cgaccgccgg tcctccggta gagaattcaa aaactttctg cccacctact
     1381 tccttgcccg agatggtagt caggcctgat ttcatcgtga atctgcattt gattccggcg
     1441 gaaagatctt ctttgaaatc aaaaagccag ttcttctcgt cggcccacct ttccgatccg
     1501 agtcccgtgc aat
//
LOCUS       DAOQJL010000730         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79787_length_1513_cov_4.094650, whole genome shotgun sequence.
ACCESSION   DAOQJL010000730 DAOQJL010000000
VERSION     DAOQJL010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79787_length_1513_cov_4.094650"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..452
                     /locus_tag="PLG78_20180"
     CDS             63..452
                     /locus_tag="PLG78_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25497.1"
                     /translation="MIWRNAGDVPYWYQEGAAYFLQSAGEARCGGTVMLSKKMAERAL
                     GRIQSKQDALPWDAVDLGDRSSALDTAALSAAFFLFAWSKRDLEGLLKTPPQMKGPPP
                     WLNDHADFEKSFRAFLETGLSKPVPGC"
     gene            complement(457..>1513)
                     /gene="rmuC"
                     /locus_tag="PLG78_20185"
     CDS             complement(457..>1513)
                     /gene="rmuC"
                     /locus_tag="PLG78_20185"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNE25498.1"
                     /translation="QTTLQKTTDSSGTRLTEMSRLQLQSLDTMRKAVEERLRALQEEN
                     VKKLDQMRATVDEKLQSTLERRLSESFRVVSDRLDQVHKGLGEMQNLAIGVGDLKKVL
                     TNVKTRGTWGEIQLSLLLEQILSPDQYQKNVRTRSGGTEFVEFAIKIPIRDEVMWLPV
                     DSKFPQELFERLGDAQEKADVELAEKASRELEAFIKKSAKEIAEKYIEPPHTTDYAIL
                     FLPTEGLYAEVIRRPGLASSIQSDYRIAIAGPTTLAALLNAFQMGFRTLAIQKRSSEA
                     WKLLEQVQHEFSKFGTLLDKTQKKLQEATDSIESARGRADKIQKRLGQAGRLRLDEGG
                     SEIEMNGDESQELLGLK"
BASE COUNT          306 a          395 c          374 g          438 t
ORIGIN      
        1 aaaattaatc ttcctcctgt gaacagattt cggacagctt tcgccatgaa ctggcgcatg
       61 ctttgatctg gcgcaacgca ggtgacgttc cctactggta ccaggaagga gcagcgtact
      121 ttttgcagtc tgcgggagaa gccagatgcg gcggaactgt gatgctttcc aaaaagatgg
      181 cagagcgcgc gcttggtagg atccaatcga aacaagacgc tctcccctgg gatgcagtcg
      241 atctgggaga tcgctccagt gccctggaca cagcggcatt gtccgctgca ttcttcttgt
      301 ttgcatggag caagcgcgat ctggaaggac tcctgaagac gccgccgcag atgaagggtc
      361 caccaccctg gctcaatgat cacgccgact tcgaaaaatc ttttcgcgcg tttcttgaaa
      421 cgggactctc caagcctgtc ccgggttgct gatgaactat ttcaagccga gtagttcctg
      481 ggattcatct ccgttcattt ctatttcgga tccaccttcg tccaggcgaa gtctgcctgc
      541 ctgacccaga cgtttttgaa ttttatcagc gcgccccctg gcgctctcta tgctatccgt
      601 tgcttcttga agtttctttt gcgttttgtc gagtaacgtc ccgaatttgc taaattcatg
      661 ctggacctgt tccagtagct tccacgcttc gctggacctt ttttgaatcg cgagcgtgcg
      721 aaagcccatt tgaaaagcat tcagaagggc ggccagtgta gtgggtcctg caatggcgat
      781 gcggtagtcc gactggatgc tcgaagcgag cccgggcctt cgaatgactt ctgcgtacag
      841 accttctgtt ggaaggaaca ggatcgcata atcggtagta tgcggaggtt caatgtattt
      901 ctctgcgatt tccttcgcgg attttttgat gaacgcttcc agttctctcg atgctttttc
      961 tgcaagttcg acgtcagctt tttcctgcgc gtcacccagc ctttcaaaca attcttgagg
     1021 aaatttggag tccaccggaa gccacatgac ttcatcgcga ataggaatct taattgcgaa
     1081 ttctacaaat tctgttccac cgcttctggt tcgcacattc ttctggtact gatccggtga
     1141 taagatttgt tccaataaaa gtgatagctg aatttctccc caggtgcccc gtgttttcac
     1201 attcgtgaga actttcttga ggtctcccac tccaatcgcc agattttgca tttctccgag
     1261 gcctttgtgt acctgatcga gtctgtcgct gaccactcgg aatgattcac tgagccgtct
     1321 ttcgagagtg gactgaagct tctcgtccac agtggcacgc atttgatcta gcttctttac
     1381 gttttcttcc tggagcgcgc gcaatcgctc ttccactgct ttgcgcattg tatcgagact
     1441 ctgcagttgc aaccgcgaca tttctgtaag tcgggttcca cttgagtctg tggttttctg
     1501 gagagtggtc tgg
//
LOCUS       DAOQJL010000731         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79892_length_1512_cov_3.856555, whole genome shotgun sequence.
ACCESSION   DAOQJL010000731 DAOQJL010000000
VERSION     DAOQJL010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79892_length_1512_cov_3.856555"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="lepB"
                     /locus_tag="PLG78_20190"
     CDS             complement(<1..1049)
                     /gene="lepB"
                     /locus_tag="PLG78_20190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE25499.1"
                     /translation="MKSRGESATSFAVSMAVLVFFVFAFKQSVLDANNIPSGSMIPTL
                     KIGDYLFVNKMRYSLRIPFTEVELIRIDDPARGDIITFIPPGDPGKNYVKRVMGMPGD
                     RIRIRSVPACDLYKAYGETLPNVPREFSCDRKSPSYRYEPQLAFVEYKEGDSGEWKNY
                     HPTELGSEEARKLLLDSDNVGVLPPELLPNFDWRGRMPVAFRENLPSGGTHLTIESSY
                     TSPNDSLCEDIETTGCVIPPNQYLAMGDNRDDSRDSRYIGYIAREKILGKVVIIYFSI
                     NWKDDICKEFARDFLRSSVPPALDDGYKIPGFPPEKQRRRCSLLDEQADSESIIDYHR
                     RTVVYRIPRMDVRWT"
     gene            complement(1088..>1512)
                     /locus_tag="PLG78_20195"
     CDS             complement(1088..>1512)
                     /locus_tag="PLG78_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE25500.1"
                     /translation="PLSFDSVLDGVYAGFAIFWPGELDVRVGEDGERRPVYLRNFICG
                     DARVRFWDLDRFWSSPPELLVYQTRQEEPDMDRAFLRFASGAGMVMLESNKLTVEQAE
                     TLKGLARQGLSYPVIQDRLRAAYPDAEIRFYFPGPVSK"
BASE COUNT          366 a          403 c          391 g          352 t
ORIGIN      
        1 ctagtccagc gcacatccat tctggggatg cgatacacga ccgtccgtct gtgataatcg
       61 atgatggatt ctgagtccgc ctgttcatcc aggagggaac agcgacgacg ctgtttttcc
      121 gggggaaatc cggggatctt gtatccatcg tccagggcag gaggcacgga agaacgcagg
      181 aaatcacgtg caaattcctt gcagatgtca tccttccagt tgatgctgaa gtagatgatc
      241 accaccttgc ccaggatctt ctctctcgct atataaccaa tgtaacgcga atcgcgcgaa
      301 tcgtctcgat tgtctcccat ggccagatac tggttaggag gaatgacgca gcctgtcgtt
      361 tcaatgtctt cacacagact gtcgttggga gacgtatacg agctttctat ggtcagatga
      421 gtgccaccgg agggcaaatt ttcgcgaaag gcaacaggca ttcttccgcg ccagtcaaag
      481 ttaggcagca gttcgggagg gagcacaccc acattgtctg aatcgagaag tagcttgcgg
      541 gcctcttctg atccaagctc ggttgggtga tagttcttcc attctccgga atccccttcc
      601 ttgtattcta caaacgccag ctgtggttcg tatctataag aaggggattt tcggtcgcag
      661 gagaattcac gaggaacatt ggggagagtc tcaccgtagg ctttatatag atcgcaggcc
      721 ggaacgctac gaatgcgaat tcgatctccg ggcattccca tcactctttt cacgtaattc
      781 tttcccgggt ctcccggagg aatgaatgtg atgatgtctc cgcgcgcagg atcatcgata
      841 cggatgagtt ccacttctgt aaaaggaatg cgcaaagaat aacgcatttt gtttacgaag
      901 aggtagtccc cgattttcag agtggggatc atggaaccgc tcgggatgtt gtttgcgtct
      961 agaactgatt gcttgaacgc gaaaacgaag aacacaagaa ccgccattga gacggcgaat
     1021 gacgtggcgc tttctccccg cgatttcact gagtccttcc tttcctctgc cgggccaggg
     1081 gaaagcatta tttgctaacc gggccaggga aatagaaacg aatctccgca tccggataag
     1141 cggcgcggag gcgatcttga atcactggat aagaaagccc ctgacgcgcc aggcctttca
     1201 atgtttccgc ctgctccacc gtgagcttgt tcgattccag catcaccata ccggcgccag
     1261 atgcgaagcg cagaaacgct ctgtccatgt ccggctcttc ctgtctggtc tggtacacga
     1321 ggagctctgg tggagaactc cagaatcgat ccagatccca gaagcgaacg cgagcatctc
     1381 cgcaaataaa attgcgcaga taaaccgggc gcctttctcc atcctcccct accctcacat
     1441 ccagttctcc aggccagaag atggcaaacc ccgcatagac tccgtccagt acagaatcaa
     1501 acgaaagcgg cg
//
LOCUS       DAOQJL010000732         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_79992_length_1511_cov_4.312500, whole genome shotgun sequence.
ACCESSION   DAOQJL010000732 DAOQJL010000000
VERSION     DAOQJL010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79992_length_1511_cov_4.312500"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..>1511
                     /locus_tag="PLG78_20200"
     CDS             7..>1511
                     /locus_tag="PLG78_20200"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNE25501.1"
                     /translation="MYRLVPEMVRIFGPFYPALKERAGEIELVIRKEEERFLRTLEQG
                     LARFKEYMAAFQAAKSPVFGGAEAFRLYDTYGFPLEMTVELAEKQGLTTDMKGFEACM
                     QEQRDRAHAHTAFKDWSFPADFKSPGESSFTGYERMQDSAKVQAILILRTKGESTDAE
                     SVPSIEAKDGPSQAILVFDHTPFYPEGGGQLGDTGQITSGQNVFTVLDTKKKQGLILH
                     IGEITHGKVSVGDSVELHVDSERRENLTHHHSATHLLNKSLRDTLGHHVSQTGSLVSP
                     DYLRFDFSHGEKIPADILRKVEEHVNAAIAKKAAVDARIMPIDQAKTAGAVATFGEKY
                     GTEVRVISMGEDGKLSRELCGGCHVPNTGNIQYFHIVKESSPGAGNRRIEAVAGSAAA
                     RFFEEAIAKLTKAISAHNDQVHASTLSADEKKAFLIEQKATTEEKSRLLGMGAAGVSP
                     LTSLLEQDAVALEKAAREYSKLSRKTQGGATLSAEEVELEKLGDLEFCART"
BASE COUNT          421 a          364 c          410 g          316 t
ORIGIN      
        1 ccaatcatgt atcgactcgt acctgagatg gttcggatct ttggaccttt ttatcctgca
       61 ctcaaggaac gcgcaggtga aatcgaactt gtgattcgca aagaagaaga aagattcctt
      121 cgcacactcg aacaggggct ggctcgtttc aaagaataca tggccgcctt tcaggcagca
      181 aaatcgcccg tctttggcgg agcagaagcg ttcagattgt atgatacata cggcttccct
      241 cttgaaatga cagtggagct tgcagaaaaa caaggtctca ctacggacat gaaaggcttc
      301 gaagcctgca tgcaagaaca aagagacaga gcgcatgcgc acactgcatt caaagactgg
      361 tcttttccag cggatttcaa atctccggga gagagttcat tcacaggtta tgagcgcatg
      421 caggattctg cgaaggtgca ggctatcttg attcttcgca caaagggaga atccacggac
      481 gcggagtctg ttccaagcat agaagccaag gacggacctt ctcaggcaat cctggtattc
      541 gatcatactc ctttttatcc ggaaggcggc ggtcagctcg gggatacggg acaaatcacc
      601 tcagggcaga atgtattcac tgtgctcgac acaaaaaaga agcaaggcct gattctccat
      661 attggagaga tcactcacgg caaagtatcc gtgggcgatt cggtggagtt gcatgtagat
      721 tcggaaaggc gtgagaatct cactcaccat cacagtgcca ctcatttgct gaacaaatct
      781 ttacgcgata cgctcgggca tcatgtttcg caaactggtt cgctggtttc gcctgactac
      841 ctgagattcg atttttcgca cggggaaaaa attcccgcag atatcctgcg caaagtggaa
      901 gaacatgtca acgcagccat cgcgaagaaa gcagcagtgg atgcgcgcat catgccgatt
      961 gaccaggcaa agacagccgg agcagtcgcc acgtttggag aaaaatacgg aacagaagtg
     1021 cgcgtcattt ccatgggaga ggatggaaag ctttcgcgag agctatgcgg tgggtgtcac
     1081 gttcccaata ccggaaacat tcagtacttc cacatcgtaa aagaaagtag tccgggtgcg
     1141 ggcaatagaa ggatcgaggc agtggcgggc tcggctgcgg ccagattctt tgaggaagcc
     1201 attgcgaaac tgacaaaagc catatcagcg cacaacgatc aggtgcatgc atcaaccttg
     1261 agtgcagatg aaaagaaggc gttcctcata gagcaaaaag caaccacaga ggaaaaatcg
     1321 aggctcctgg gaatgggagc ggccggagtg tctcctctta cttctcttct ggaacaggat
     1381 gctgtggcgc ttgagaaagc ggcgcgcgaa tactccaagc tctcgcgcaa aacccaggga
     1441 ggagcaaccc tttccgcgga ggaagtcgag ctagaaaagc tcggagacct ggaattctgc
     1501 gcgcgcacat t
//
LOCUS       DAOQJL010000733         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0601
            NODE_80122_length_1510_cov_3.635052, whole genome shotgun sequence.
ACCESSION   DAOQJL010000733 DAOQJL010000000
VERSION     DAOQJL010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810233
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,041
            CDSs (total)                      :: 4,011
            Genes (coding)                    :: 3,988
            CDSs (with protein)               :: 3,988
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 0 of 23
            Pseudo Genes (incomplete)         :: 22 of 23
            Pseudo Genes (internal stop)      :: 1 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80122_length_1510_cov_3.635052"
                     /isolate="AMR_MDS_0601"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PLG78_20205"
     CDS             complement(<1..1466)
                     /locus_tag="PLG78_20205"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNE25502.1"
                     /translation="MAFSKSPDVAKDSDFRPVKIGYWQDQGYSATGFATYRMRLNCRP
                     EKDTIRIYESSAAAAAYRNGTMLASSGTPGKTAEEERPRIAPWSVPIHEGDTLFYVSN
                     HVARGGGILGYRFGKETDLEAERARSLYQEAMVLGMLNAAGFFFLIYFLARGGGAALP
                     VFTLLMIVCTVRSAASNSVLEEWFPDRQLTDLRLRLEYLTALALMPPLYFWAIALLFQ
                     GDPETSPATLRRTITRILSAVYSIGAFCLSLFFIFARDASVYGKYQPEYVSCYLLPGL
                     AVCLFLLFILVIEKRRGAGWMLLGFLSVLFSTLADAETTVAGQAGTLYVPLGLLGFAA
                     CFSVTVGIRIRSDFEELALTRKNLALSFEQDRRREQKRASEREQIALQAVSALSRTAS
                     DFEEMLSQGKVQKDAAIPALSRLHSLRVRLEKRLSRTPVAPVALDVLRYIRFHHPSVR
                     IPSQASLVVFSSRRELDRFIGEIISIHDSACTSEIVLLR"
BASE COUNT          401 a          397 c          391 g          321 t
ORIGIN      
        1 cgaaggagta caatttcaga cgtacacgca ctgtcatgaa tgcttatgat ttccccgatg
       61 aaacggtcga gctctcgcct gcttgaaaaa accacaaggc tggcctgaga tgggatgcga
      121 acagagggat gatgaaagcg aatgtagcga agaacatcga gcgcgaccgg agccactgga
      181 gttcgactca acctcttctc aagccggacg cgcaatgaat gcaatctgga aagagcaggg
      241 atggccgcgt ccttttgcac tttgccctga ctgagcatct cttcaaaatc agaggcagtg
      301 cgcgaaagag cggacactgc ctggagtgca atttgttcac gctcggaggc ccttttctgc
      361 tctcttctcc tgtcttgctc aaagctgagc gccagattct ttctggtgag ggcgagttcc
      421 tcgaagtcgc ttctgattct aatcccgacc gtgacagaaa agcaggctgc gaatccaaga
      481 agtcccagag gcacgtagag tgttcccgcc tggccggcta cagtggtttc tgcatccgcc
      541 agagtagaga acaaaacact caggaaacca agaagcatcc atccggcccc tctgcgtttt
      601 tcaatcacaa gtatgaaaag caagaacaga caaacagcaa gccccggcag aagatagcag
      661 ctaacgtatt cgggttgata ctttccgtaa acggaggcgt cgcgtgcgaa gatgaaaaat
      721 agactcaggc aaaacgcacc gatcgaatag actgcggata gaatgcgcgt aatcgtgcga
      781 cgcagtgtcg cagggctcgt ctcaggatct ccctgaaaca aaagagcaat cgcccagaag
      841 taaagtggag gcataagggc aagcgccgtg aggtattcca ggcgcaggcg aagatccgtt
      901 agctggcgat ccggaaacca ttcttccaga acactgttgg atgcggcact tctcacggtg
      961 cacacaatca tcaacagagt aaagactgga agggcggcgc caccaccgcg cgcgagaaag
     1021 tagatcagaa agaagaaccc ggcggcgttg agcataccaa gaaccatagc ctcctggtag
     1081 agagacctgg cacgctctgc ctctaaatca gtttctttgc caaatctgta tccaagaatt
     1141 cctccgcctc gcgccacgtg attggatacg taaaacaaag tatctccttc atgtatagga
     1201 acagaccagg gcgcaatgcg tggcctttct tcctctgcgg tttttccagg agttccagac
     1261 gaagcgagca tcgtgccgtt tctgtaagcg gccgccgcag cagaggattc atagattcga
     1321 atggtgtctt tttccggtct gcaattcaga cgcattcggt acgttgcaaa gccagtcgcg
     1381 ctatatccct gatcctgcca gtatccaatc tttaccggtc tgaaatctga atcttttgca
     1441 acgtcaggcg actttgagaa tgccatttcc cagacaacgc gatccagaca gggagtttct
     1501 tctttcaggc
//
